WorldWideScience

Sample records for activity graphical profiles

  1. Quantitative genetic activity graphical profiles for use in chemical evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Waters, M.D. [Environmental Protection Agency, Washington, DC (United States); Stack, H.F.; Garrett, N.E.; Jackson, M.A. [Environmental Health Research and Testing, Inc., Research Triangle Park, NC (United States)

    1990-12-31

    A graphic approach, terms a Genetic Activity Profile (GAP), was developed to display a matrix of data on the genetic and related effects of selected chemical agents. The profiles provide a visual overview of the quantitative (doses) and qualitative (test results) data for each chemical. Either the lowest effective dose or highest ineffective dose is recorded for each agent and bioassay. Up to 200 different test systems are represented across the GAP. Bioassay systems are organized according to the phylogeny of the test organisms and the end points of genetic activity. The methodology for producing and evaluating genetic activity profile was developed in collaboration with the International Agency for Research on Cancer (IARC). Data on individual chemicals were compiles by IARC and by the US Environmental Protection Agency (EPA). Data are available on 343 compounds selected from volumes 1-53 of the IARC Monographs and on 115 compounds identified as Superfund Priority Substances. Software to display the GAPs on an IBM-compatible personal computer is available from the authors. Structurally similar compounds frequently display qualitatively and quantitatively similar profiles of genetic activity. Through examination of the patterns of GAPs of pairs and groups of chemicals, it is possible to make more informed decisions regarding the selection of test batteries to be used in evaluation of chemical analogs. GAPs provided useful data for development of weight-of-evidence hazard ranking schemes. Also, some knowledge of the potential genetic activity of complex environmental mixtures may be gained from an assessment of the genetic activity profiles of component chemicals. The fundamental techniques and computer programs devised for the GAP database may be used to develop similar databases in other disciplines. 36 refs., 2 figs.

  2. Mathematical Creative Activity and the Graphic Calculator

    Science.gov (United States)

    Duda, Janina

    2011-01-01

    Teaching mathematics using graphic calculators has been an issue of didactic discussions for years. Finding ways in which graphic calculators can enrich the development process of creative activity in mathematically gifted students between the ages of 16-17 is the focus of this article. Research was conducted using graphic calculators with…

  3. Ring-tool profiling - graphical method in CATIA based on Generating trajectories theorem

    Science.gov (United States)

    Frumuşanu, G.; Teodor, V.; Oancea, N.

    2016-11-01

    Machining of threads having high dimensions and multiple starts by turning is a challenging problem. An alternative possibility is to machine them by milling. The most productive milling solution is when using tools with inner active surface, namely ring tools. In the case of threads with multiple starts, the reciprocal enwrapped profile of the ring tool is considerably different to the shape of the thread axial (normal) section. In this paper, we suggest a methodology to profile the generator ring tool, based on a complementary theorem from enwrapped surfaces field. At the same time, a graphical algorithm aiming to find the ring tool profile, developed in CATIA graphical environment has been applied in the concrete case of a trapezoidal thread. The graphical profiling solution is presented in comparison to an analytical solution, in order to test the results precision. The graphical profiling method proves to be rigorous, easy to apply and highly intuitive.

  4. Graphical method for profiling hob mill that generate cycloid worms

    Science.gov (United States)

    Teodor, V.; Berbinschi, S.; Baroiu, N.; Oancea, N.

    2015-11-01

    The hob mill for generating ordered curls of cycloid surface with non involute profiles may be profiled based on the fundamental theorems of surface enveloping - Olivier - as surface reciprocally enveloping with point like contact. In this paper, is proposed a methodology based on a complementary theorem of the surface enveloping in a graphical expression developed in a graphical design environment - CATIA. The graphical method presented in this paper is developed in two stages: determining of the rack gear model based on the solid model of the surface to be generated, using an original algorithm, following this, based on 3D modelling is determined the solid model of the primary peripheral surface of the hob mill. An application for a cycloid worm is presented - a central screw of helical pumps. In order to prove the quality of method, the analytical and graphical solutions are comparatively presented.

  5. Analysis of graphic representations of activity theory in international journals

    Directory of Open Access Journals (Sweden)

    Marco André Mazzarotto

    2016-05-01

    Full Text Available Activity theory is a relevant framework for the Design field, and their graphic representations are cognitive artifacts that aid the understanding, use and communication of this theory. However, there is a lack of consistency around the graphics and labels used in these representations. Based on this, the aim of this study was to identify, analyze and evaluate these differences and propose a representation that aims to be more suitable for the theory. For this, uses as method a literature review based on Engeström (2001 and its three generations of visual models, combined with graphical analysis of representations collected in a hundred papers from international journals.

  6. Cereal Box Design: An Interdisciplinary Graphics Activity

    Science.gov (United States)

    Fitzgerald, Mike; Tsosie, Teri

    2012-01-01

    The cereal box design activity is intriguing both for its simplicity and the resourcefulness that it can generate in young people. Also, it lends itself to a variety of curriculums. It covers both consumerism and Design for the Environment (DfE) concepts broadly and in depth. The activity introduces a wide range of topics. They include graphic…

  7. Graphical user interface (GUI for design of passenger car suspension system using random road profile

    Directory of Open Access Journals (Sweden)

    Duna Tariq Yaseen

    2016-01-01

    Full Text Available In this paper, an Interactive approach has been introduced for the design of Passenger car suspension system subjected to random road profile, by building a graphical user interface (GUI, using Matlab/Guide has been presented. The aim of the work is to show the importance and usefulness of the developed GUI in designing and describing the dynamic behavior of car suspension system for different design criteria. Common problems in the field of design of suspension systems for the quarter-car passive model are analyzed. The result shows that the designed GUI is very convenient for engineers, analysts, and designers of car suspension systems.

  8. Brain Activities Associated with Graphic Emoticons: An fMRI Study

    Science.gov (United States)

    Yuasa, Masahide; Saito, Keiichi; Mukawa, Naoki

    In this paper, we describe the brain activities that are associated with graphic emoticons by using functional MRI (fMRI). We use various types of faces from abstract to photorealistic in computer network applications. A graphics emoticon is an abstract face in communication over computer network. In this research, we created various graphic emoticons for the fMRI study and the graphic emoticons were classified according to friendliness and level of arousal. We investigated the brain activities of participants who were required to evaluate the emotional valence of the graphic emoticons (happy or sad). The experimental results showed that not only the right inferior frontal gyrus and the cingulate gyrus, but also the inferior and middle temporal gyrus and the fusiform gyrus, were found to be activated during the experiment. Forthermore, it is possible that the activation of the right inferior frontal gyrus and the cingulate gyrus is related to the type of abstract face. Since the inferior and middle temporal gyrus were activated, even though the graphic emoticons are static, we may perceive graphic emoticons as dynamic and living agents. Moreover, it is believed that text and graphics emoticons play an important role in enriching communication among users.

  9. A graphical simulator for active learning of MRI basics

    DEFF Research Database (Denmark)

    Wilhjelm, Jens E.; Duun-Henriksen, Jonas; Hanson, Lars G.

    2014-01-01

    This paper presents a MATLAB-based graphical user interface (GUI) for simulation of a simple magnetic resonance imaging (MRI) scanner that the student can operate and obtain results with. It is intended for the students in an introductory course in medical imaging and provides the students...

  10. Sweet! Candy Bar Activity Teaches CAD, Math, and Graphics

    Science.gov (United States)

    Granlund, George

    2009-01-01

    By far, the tastiest technology learning activity that the author's students work on is the development of the design of a chocolate candy bar. This article describes how the author implemented the candy bar activity. The activity gives students an opportunity to design a product and to take it from concept through to production.

  11. A novel R-package graphic user interface for the analysis of metabonomic profiles

    Directory of Open Access Journals (Sweden)

    Villa Palmira

    2009-10-01

    Full Text Available Abstract Background Analysis of the plethora of metabolites found in the NMR spectra of biological fluids or tissues requires data complexity to be simplified. We present a graphical user interface (GUI for NMR-based metabonomic analysis. The "Metabonomic Package" has been developed for metabonomics research as open-source software and uses the R statistical libraries. Results The package offers the following options: Raw 1-dimensional spectra processing: phase, baseline correction and normalization. Importing processed spectra. Including/excluding spectral ranges, optional binning and bucketing, detection and alignment of peaks. Sorting of metabolites based on their ability to discriminate, metabolite selection, and outlier identification. Multivariate unsupervised analysis: principal components analysis (PCA. Multivariate supervised analysis: partial least squares (PLS, linear discriminant analysis (LDA, k-nearest neighbor classification. Neural networks. Visualization and overlapping of spectra. Plot values of the chemical shift position for different samples. Furthermore, the "Metabonomic" GUI includes a console to enable other kinds of analyses and to take advantage of all R statistical tools. Conclusion We made complex multivariate analysis user-friendly for both experienced and novice users, which could help to expand the use of NMR-based metabonomics.

  12. Systems Biology Graphical Notation: Activity Flow language Level 1 Version 1.2.

    Science.gov (United States)

    Mi, Huaiyu; Schreiber, Falk; Moodie, Stuart; Czauderna, Tobias; Demir, Emek; Haw, Robin; Luna, Augustin; Le Novère, Nicolas; Sorokin, Anatoly; Villéger, Alice

    2015-09-04

    The Systems Biological Graphical Notation (SBGN) is an international community effort for standardized graphical representations of biological pathways and networks. The goal of SBGN is to provide unambiguous pathway and network maps for readers with different scientific backgrounds as well as to support efficient and accurate exchange of biological knowledge between different research communities, industry, and other players in systems biology. Three SBGN languages, Process Description (PD), Entity Relationship (ER) and Activity Flow (AF), allow for the representation of different aspects of biological and biochemical systems at different levels of detail. The SBGN Activity Flow language represents the influences of activities among various entities within a network. Unlike SBGN PD and ER that focus on the entities and their relationships with others, SBGN AF puts the emphasis on the functions (or activities) performed by the entities, and their effects to the functions of the same or other entities. The nodes (elements) describe the biological activities of the entities, such as protein kinase activity, binding activity or receptor activity, which can be easily mapped to Gene Ontology molecular function terms. The edges (connections) provide descriptions of relationships (or influences) between the activities, e.g., positive influence and negative influence. Among all three languages of SBGN, AF is the closest to signaling pathways in biological literature and textbooks, but its well-defined semantics offer a superior precision in expressing biological knowledge.

  13. Profiling procedure for disk cutter to generate the male rotor, screw compressors component, using the “Substitute Family Circle” - graphic method in AUTOCAD environment

    Science.gov (United States)

    Popa, CL; Popa, V.

    2016-11-01

    This paper proposes a profiling method for the tool which generates the helical groove of male rotor, screw compressor component. The method is based on a complementary theorem of surfaces enveloping - "Substitute Family Circles Method”. The specific theorem of family circles of substitution has been applied using AUTOCAD graphics design environment facility. The frontal view of the male rotor, screw compressor component, has been determinate knowing the transverse profile of female rotor, and using this theorem of "Substitute Family Circle". The three-dimensional model of the rotor makes possible to apply the same theorem, leading to the surface of revolution enveloping the helical surface. An application will be also presented to determine the axial profile of the disk cutter, numeric and graphics, following the proposed algorithm.

  14. The computer graphics metafile

    CERN Document Server

    Henderson, LR; Shepherd, B; Arnold, D B

    1990-01-01

    The Computer Graphics Metafile deals with the Computer Graphics Metafile (CGM) standard and covers topics ranging from the structure and contents of a metafile to CGM functionality, metafile elements, and real-world applications of CGM. Binary Encoding, Character Encoding, application profiles, and implementations are also discussed. This book is comprised of 18 chapters divided into five sections and begins with an overview of the CGM standard and how it can meet some of the requirements for storage of graphical data within a graphics system or application environment. The reader is then intr

  15. The computer graphics interface

    CERN Document Server

    Steinbrugge Chauveau, Karla; Niles Reed, Theodore; Shepherd, B

    2014-01-01

    The Computer Graphics Interface provides a concise discussion of computer graphics interface (CGI) standards. The title is comprised of seven chapters that cover the concepts of the CGI standard. Figures and examples are also included. The first chapter provides a general overview of CGI; this chapter covers graphics standards, functional specifications, and syntactic interfaces. Next, the book discusses the basic concepts of CGI, such as inquiry, profiles, and registration. The third chapter covers the CGI concepts and functions, while the fourth chapter deals with the concept of graphic obje

  16. On active current selection for Lagrangian profilers

    DEFF Research Database (Denmark)

    Jouffroy, Jerome; Zhou, Qiuyang; Zielinski, Oliver

    2013-01-01

    simple and computationally-efficient control strategies to actively select and use ocean currents so that a profiler can autonomously reach a desired destination. After briefly presenting a typical profiler and possible mechanical modifications for a coastal environment, we introduce simple mathematical...... models for the profiler and the currents it will use. We then present simple feedback controllers that, using the direction of the currents and taking into account the configuration of the environment (coastal or deep-sea), is able to steer the profiler to any desired horizontal location. To illustrate...... the approach, a few results are presented using both simulated currents and real current velocities from the North Sea....

  17. HT-BONE: a graphical user interface for the identification of bone profiles in CT images via extended Hough transform

    Science.gov (United States)

    Campi, Cristina; Perasso, Annalisa; Beltrametti, Mauro C.; Piana, Michele; Sambuceti, Gianmario; Massone, Anna Maria

    2016-03-01

    It has been recently proved that the computational analysis of X-ray Computed Tomography (CT) images allows clinicians to assess the alteration of compact bone asset due to hematological diseases. HT-BONE implements a new method, based on an extension of the Hough transform (HT) to a wide class of algebraic curves, for accurately measuring global and regional geometric properties of trabecular and compact bone districts. In the case of CT/PET analysis, the segmentation of the CT images provides masks for Positron Emission Tomography (PET) data, extracting the metabolic activity in the region surrounded by compact bone tissue. HT-BONE offers an intuitive, user-friendly, Matlab-based Graphical User Interface (GUI) for all input/output procedures and the automatic managing of the segmentation process also from non-expert users: the CT/PET data can be loaded and browsed easily and the only pre-preprocessing required from the user is the drawing of Regions Of Interest (ROIs) around the bone districts under consideration. For each bone district, specific families of curves, whose reliability has been already tested in previous works, is automatically selected for the recognition task via HT. As output, the software returns masks of the segmented compact bone regions, images of the Standard Uptake Values (SUV) in the masked regions of PET slices, and the values of the parameters in the curve equations utilized in the HT procedure. This information can be used for all pathologies and clinical conditions for which the alteration of the compact bone asset or bone marrow distribution plays a crucial role.

  18. R graphics

    CERN Document Server

    Murrell, Paul

    2005-01-01

    R is revolutionizing the world of statistical computing. Powerful, flexible, and best of all free, R is now the program of choice for tens of thousands of statisticians. Destined to become an instant classic, R Graphics presents the first complete, authoritative exposition on the R graphical system. Paul Murrell, widely known as the leading expert on R graphics, has developed an in-depth resource that takes nothing for granted and helps both neophyte and seasoned users master the intricacies of R graphics. After an introductory overview of R graphics facilities, the presentation first focuses

  19. T & I--Graphic Arts, Silk Screen Printing. Kit No. 60. Instructor's Manual [and] Student Learning Activity Guide.

    Science.gov (United States)

    Cope, George

    An instructor's manual and student activity guide on silk screen printing are provided in this set of prevocational education materials which focuses on the vocational area of trade and industry (graphic arts). (This set of materials is one of ninety-two prevocational sets arranged around a cluster of seven vocational offerings: agriculture, home…

  20. NATURAL graphics

    Science.gov (United States)

    Jones, R. H.

    1984-01-01

    The hardware and software developments in computer graphics are discussed. Major topics include: system capabilities, hardware design, system compatibility, and software interface with the data base management system.

  1. Graphic Storytelling

    Science.gov (United States)

    Thompson, John

    2009-01-01

    Graphic storytelling is a medium that allows students to make and share stories, while developing their art communication skills. American comics today are more varied in genre, approach, and audience than ever before. When considering the impact of Japanese manga on the youth, graphic storytelling emerges as a powerful player in pop culture. In…

  2. Graphics gems

    CERN Document Server

    Glassner, Andrew S

    1993-01-01

    ""The GRAPHICS GEMS Series"" was started in 1990 by Andrew Glassner. The vision and purpose of the Series was - and still is - to provide tips, techniques, and algorithms for graphics programmers. All of the gems are written by programmers who work in the field and are motivated by a common desire to share interesting ideas and tools with their colleagues. Each volume provides a new set of innovative solutions to a variety of programming problems.

  3. Graphic Review

    DEFF Research Database (Denmark)

    Breiting, Søren

    2002-01-01

    Introduktion til 'graphic review' som en metode til at føre forståelse fra en undervisngsgang til den næste i læreruddannelse og grundskole.......Introduktion til 'graphic review' som en metode til at føre forståelse fra en undervisngsgang til den næste i læreruddannelse og grundskole....

  4. Activity-Based Protein Profiling of Microbes

    Energy Technology Data Exchange (ETDEWEB)

    Sadler, Natalie C.; Wright, Aaron T.

    2015-02-01

    Activity-Based Protein Profiling (ABPP) in conjunction with multimodal characterization techniques has yielded impactful findings in microbiology, particularly in pathogen, bioenergy, drug discovery, and environmental research. Using small molecule chemical probes that react irreversibly with specific proteins or protein families in complex systems has provided insights in enzyme functions in central metabolic pathways, drug-protein interactions, and regulatory protein redox, for systems ranging from photoautotrophic cyanobacteria to mycobacteria, and combining live cell or cell extract ABPP with proteomics, molecular biology, modeling, and other techniques has greatly expanded our understanding of these systems. New opportunities for application of ABPP to microbial systems include: enhancing protein annotation, characterizing protein activities in myriad environments, and reveal signal transduction and regulatory mechanisms in microbial systems.

  5. Graphical processing unit implementation of an integrated shape-based active contour: Application to digital pathology

    Directory of Open Access Journals (Sweden)

    Sahirzeeshan Ali

    2011-01-01

    Full Text Available Commodity graphics hardware has become a cost-effective parallel platform to solve many general computational problems. In medical imaging and more so in digital pathology, segmentation of multiple structures on high-resolution images, is often a complex and computationally expensive task. Shape-based level set segmentation has recently emerged as a natural solution to segmenting overlapping and occluded objects. However the flexibility of the level set method has traditionally resulted in long computation times and therefore might have limited clinical utility. The processing times even for moderately sized images could run into several hours of computation time. Hence there is a clear need to accelerate these segmentations schemes. In this paper, we present a parallel implementation of a computationally heavy segmentation scheme on a graphical processing unit (GPU. The segmentation scheme incorporates level sets with shape priors to segment multiple overlapping nuclei from very large digital pathology images. We report a speedup of 19× compared to multithreaded C and MATLAB-based implementations of the same scheme, albeit with slight reduction in accuracy. Our GPU-based segmentation scheme was rigorously and quantitatively evaluated for the problem of nuclei segmentation and overlap resolution on digitized histopathology images corresponding to breast and prostate biopsy tissue specimens.

  6. Graphics gems

    CERN Document Server

    Heckbert, Paul S

    1994-01-01

    Graphics Gems IV contains practical techniques for 2D and 3D modeling, animation, rendering, and image processing. The book presents articles on polygons and polyhedral; a mix of formulas, optimized algorithms, and tutorial information on the geometry of 2D, 3D, and n-D space; transformations; and parametric curves and surfaces. The text also includes articles on ray tracing; shading 3D models; and frame buffer techniques. Articles on image processing; algorithms for graphical layout; basic interpolation methods; and subroutine libraries for vector and matrix algebra are also demonstrated. Com

  7. Graphic Ecologies

    Directory of Open Access Journals (Sweden)

    Brook Weld Muller

    2014-12-01

    Full Text Available This essay describes strategic approaches to graphic representation associated with critical environmental engagement and that build from the idea of works of architecture as stitches in the ecological fabric of the city. It focuses on the building up of partial or fragmented graphics in order to describe inclusive, open-ended possibilities for making architecture that marry rich experience and responsive performance. An aphoristic approach to crafting drawings involves complex layering, conscious absence and the embracing of tension. A self-critical attitude toward the generation of imagery characterized by the notion of ‘loose precision’ may lead to more transformative and environmentally responsive architectures.

  8. 用作图法绘制凸轮靠模的轮廓曲线%Drawing Profile Curve of Cam Profile Modeling with Graphical Solution

    Institute of Scientific and Technical Information of China (English)

    徐建军; 包轩庭

    2012-01-01

      This paper proposes a method of using a drawing to determine the profile curve of cam profile model⁃ing. It is a simpler solution to grinding cam, which has a spline curve contour.%  提出了一种用作图法来确定凸轮靠模轮廓曲线的方法,比较简便地解决了轮廓为样条曲线的凸轮的磨削问题。

  9. Graphic notation

    DEFF Research Database (Denmark)

    Bergstrøm-Nielsen, Carl

    2010-01-01

    Graphic notation is taught to music therapy students at Aalborg University in both simple and elaborate forms. This is a method of depicting music visually, and notations may serve as memory aids, as aids for analysis and reflection, and for communication purposes such as supervision or within...

  10. On Active Current Selection for Lagrangian Profilers

    Directory of Open Access Journals (Sweden)

    J. Jouffroy

    2013-01-01

    Full Text Available Autonomous Lagrangian profilers are now widely used as measurement and monitoring platforms, notably in observation programs as Argo. In a typical mode of operation, the profilers drift passively at their parking depthbefore making a vertical profile to go back to the surface. This paperpresents simple and computationally-efficient control strategies to activelyselect and use ocean currents so that a profiler can autonomously reach adesired destination. After briefly presenting a typical profiler andpossible mechanical modifications for a coastal environment, we introducesimple mathematical models for the profiler and the currents it will use. Wethen present simple feedback controllers that, using the direction of thecurrents and taking into account the configuration of the environment(coastal or deep-sea, is able to steer the profiler to any desiredhorizontal location. To illustrate the approach, a few results are presentedusing both simulated currents and real current velocity profiles from theNorth Sea.

  11. Resurfacing Graphics

    Directory of Open Access Journals (Sweden)

    Prof. Patty K. Wongpakdee

    2013-06-01

    Full Text Available “Resurfacing Graphics” deals with the subject of unconventional design, with the purpose of engaging the viewer to experience the graphics beyond paper’s passive surface. Unconventional designs serve to reinvigorate people, whose senses are dulled by the typical, printed graphics, which bombard them each day. Today’s cutting-edge designers, illustrators and artists utilize graphics in a unique manner that allows for tactile interaction. Such works serve as valuable teaching models and encourage students to do the following: 1 investigate the trans-disciplines of art and technology; 2 appreciate that this approach can have a positive effect on the environment; 3 examine and research other approaches of design communications and 4 utilize new mediums to stretch the boundaries of artistic endeavor. This paper examines how visuals communicators are “Resurfacing Graphics” by using atypical surfaces and materials such as textile, wood, ceramics and even water. Such non-traditional transmissions of visual language serve to demonstrate student’s overreliance on paper as an outdated medium. With this exposure, students can become forward-thinking, eco-friendly, creative leaders by expanding their creative breadth and continuing the perpetual exploration for new ways to make their mark.

  12. Resurfacing Graphics

    Directory of Open Access Journals (Sweden)

    Prof. Patty K. Wongpakdee

    2013-06-01

    Full Text Available “Resurfacing Graphics” deals with the subject of unconventional design, with the purpose of engaging the viewer to experience the graphics beyond paper’s passive surface. Unconventional designs serve to reinvigorate people, whose senses are dulled by the typical, printed graphics, which bombard them each day. Today’s cutting-edge designers, illustrators and artists utilize graphics in a unique manner that allows for tactile interaction. Such works serve as valuable teaching models and encourage students to do the following: 1 investigate the trans-disciplines of art and technology; 2 appreciate that this approach can have a positive effect on the environment; 3 examine and research other approaches of design communications and 4 utilize new mediums to stretch the boundaries of artistic endeavor. This paper examines how visuals communicators are “Resurfacing Graphics” by using atypical surfaces and materials such as textile, wood, ceramics and even water. Such non-traditional transmissions of visual language serve to demonstrate student’s overreliance on paper as an outdated medium. With this exposure, students can become forward-thinking, eco-friendly, creative leaders by expanding their creative breadth and continuing the perpetual exploration for new ways to make their mark. 

  13. A MATLAB-based graphical user interface for the identification of muscular activations from surface electromyography signals.

    Science.gov (United States)

    Mengarelli, Alessandro; Cardarelli, Stefano; Verdini, Federica; Burattini, Laura; Fioretti, Sandro; Di Nardo, Francesco

    2016-08-01

    In this paper a graphical user interface (GUI) built in MATLAB® environment is presented. This interactive tool has been developed for the analysis of superficial electromyography (sEMG) signals and in particular for the assessment of the muscle activation time intervals. After the signal import, the tool performs a first analysis in a totally user independent way, providing a reliable computation of the muscular activation sequences. Furthermore, the user has the opportunity to modify each parameter of the on/off identification algorithm implemented in the presented tool. The presence of an user-friendly GUI allows the immediate evaluation of the effects that the modification of every single parameter has on the activation intervals recognition, through the real-time updating and visualization of the muscular activation/deactivation sequences. The possibility to accept the initial signal analysis or to modify the on/off identification with respect to each considered signal, with a real-time visual feedback, makes this GUI-based tool a valuable instrument in clinical, research applications and also in an educational perspective.

  14. Functional-graphical method of solving problems in the implementation of a differentiated approach to process of training of pupils for the Unified State Exam in mathematics (profile level

    Directory of Open Access Journals (Sweden)

    Olga Vergazova

    2016-04-01

    Full Text Available The problem of raising the level of mathematical preparation of students of mathematical and technical specialties should be addressed to both the course of school level algebra and analysis, and the preparation to the Unified State Examination in mathematics (profile level. The example of the application of the functional-graphical method for solving trigonometric equations and inequalities in problems of high level on the use math level profile, demonstrates one of the possibilities of differentiated approach in training future first-year student of technical or mathematical University. For example, the decision of tasks demonstrates the advantages of using functional-graphical method for solving trigonometric equations and inequalities in the formation and development of skills of analysis and graphing of elementary functions. The paper is of interest to academicians, high school teachers and students wishing to enter technical or mathematical university.

  15. Design Graphics

    Science.gov (United States)

    1990-01-01

    A mathematician, David R. Hedgley, Jr. developed a computer program that considers whether a line in a graphic model of a three-dimensional object should or should not be visible. Known as the Hidden Line Computer Code, the program automatically removes superfluous lines and displays an object from a specific viewpoint, just as the human eye would see it. An example of how one company uses the program is the experience of Birdair which specializes in production of fabric skylights and stadium covers. The fabric called SHEERFILL is a Teflon coated fiberglass material developed in cooperation with DuPont Company. SHEERFILL glazed structures are either tension structures or air-supported tension structures. Both are formed by patterned fabric sheets supported by a steel or aluminum frame or cable network. Birdair uses the Hidden Line Computer Code, to illustrate a prospective structure to an architect or owner. The program generates a three- dimensional perspective with the hidden lines removed. This program is still used by Birdair and continues to be commercially available to the public.

  16. Inter-level Scaffolding and Sequences of Representational Activities in Teaching a Chemical System with Graphical Simulations

    Science.gov (United States)

    Li, Na; Black, John B.

    2016-10-01

    Chemistry knowledge can be represented at macro-, micro- and symbolic levels, and learning a chemistry topic requires students to engage in multiple representational activities. This study focused on scaffolding for inter-level connection-making in learning chemistry knowledge with graphical simulations. We also tested whether different sequences of representational activities produced different student learning outcomes in learning a chemistry topic. A sample of 129 seventh graders participated in this study. In a simulation-based environment, participants completed three representational activities to learn several ideal gas law concepts. We conducted a 2 × 3 factorial design experiment. We compared two scaffolding conditions: (1) the inter- level scaffolding condition in which participants received inter-level questions and experienced the dynamic link function in the simulation-based environment and (2) the intra- level scaffolding condition in which participants received intra-level questions and did not experience the dynamic link function. We also compared three different sequences of representational activities: macro-symbolic-micro, micro-symbolic-macro and symbolic-micro-macro. For the scaffolding variable, we found that the inter- level scaffolding condition produced significantly better performance in both knowledge comprehension and application, compared to the intra- level scaffolding condition. For the sequence variable, we found that the macro-symbolic-micro sequence produced significantly better knowledge comprehension performance than the other two sequences; however, it did not benefit knowledge application performance. There was a trend that the treatment group who experienced inter- level scaffolding and the micro-symbolic-macro sequence achieved the best knowledge application performance.

  17. Metabolomic Profiling of Prostate Cancer Progression During Active Surveillance

    Science.gov (United States)

    2012-10-01

    cancer or a history of transurethral resection of the prostate (TURP) for benign prostatic hypertrophy are excluded. Somewhat surprisingly...AD_________________ Award Number: W81XWH-11-1-0451 TITLE: Metabolomic Profiling of Prostate Cancer...29 September 2012 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER Metabolomic Profiling of Prostate Cancer Progression During Active Surveillance 5b

  18. Multicomponent He I 10830 {\\AA} profiles in an active filament

    CERN Document Server

    Sasso, C; Solanki, S K

    2011-01-01

    We present new spectropolarimetric observations of the chromospheric He I 10830 {\\AA} multiplet observed in a filament during its phase of activity. The data were recorded with the new Tenerife Infrared Polarimeter (TIP-II) at the German Vacuum Tower Telescope (VTT) on 2005 May 18. We inverted the He Stokes profiles using multiple atmospheric components. The observed He Stokes profiles display a remarkably wide variety of shapes. Most of the profiles show very broad Stokes I absorptions and complex and spatially variable Stokes V signatures. The inversion of the profiles shows evidence of different atmospheric blue- and redshifted components of the He I lines within the resolution element (1 arcsec), with supersonic velocities of up to 100 km/s. Up to five different atmospheric components are found in the same profile. We show that even these complex profiles can be reliably inverted.

  19. [Novel mathematical approach to determination of antibiotics concentration by agar diffusion method. Analysis of graphical, table and mathematical options of relative biological activity estimation].

    Science.gov (United States)

    Esipov, S E

    1998-01-01

    Several varieties of relative biological activity estimation are comparatively analyzed and illustrated by an example of tylosin. For visual demonstration the estimates with the equation of a straight line are represented graphically. It is concluded that the design equations in the State Pharmacopeia XI (USSR) should be respectively replaced. The advantages of the variety for the biological activity estimation with the one-point intercept form of the equation of a straight line are illustrated by particular examples.

  20. Lunar seismic profiling experiment natural activity study

    Science.gov (United States)

    Duennebier, F. K.

    1976-01-01

    The Lunar Seismic Experiment Natural Activity Study has provided a unique opportunity to study the high frequency (4-20 Hz) portion to the seismic spectrum on the moon. The data obtained from the LSPE was studied to evaluate the origin and importance of the process that generates thermal moonquakes and the characteristics of the seismic scattering zone at the lunar surface. The detection of thermal moonquakes by the LSPE array made it possible to locate the sources of many events and determine that they are definitely not generated by astronaut activities but are the result of a natural process on the moon. The propagation of seismic waves in the near-surface layers was studied in a qualitative manner. In the absence of an adequate theoretical model for the propagation of seismic waves in the moon, it is not possible to assign a depth for the scattering layer. The LSPE data does define several parameters which must be satisfied by any model developed in the future.

  1. Accounting for Students' Schemes in the Development of a Graphical Process for Solving Polynomial Inequalities in Instrumented Activity

    Science.gov (United States)

    Rivera, Ferdinand D.

    2007-01-01

    This paper provides an instrumental account of precalculus students' graphical process for solving polynomial inequalities. It is carried out in terms of the students' instrumental schemes as mediated by handheld graphing calculators and in cooperation with their classmates in a classroom setting. The ethnographic narrative relays an instrumental…

  2. Graphical Abstract

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Effects of Barium Atoms on Adsorption and Dissociation of Nitrogen Molecules on Ru(0001) Surface First principles calculation had been performed to present the effects of barium atom on dissociation of nitrogen molecule on Ru(0001) surface. The barrier energy of nitrogen molecular dissociation on Ru(0001) surface was reduced from 2.06 eV to 1,17 eV by effects of barium atom. Activities of Ru(0001) terrace surface were magnified about 9 orders at 500 K.

  3. Profiles and Pauses: Two Practical Activities for the Writing Class

    Directory of Open Access Journals (Sweden)

    Ernest Hall

    1998-01-01

    Full Text Available Abstract : This article describes two classroom activities, "Profiling" and "Pause Analysis", that can be successfully used in ESL writing classes. "Profiling" addresses such problems as poor development of ideas, simplistic ideas, and lack of coherence in written texts. "Pause Analysis" focusses on the thinking processes that students engage in while drafting text, processes such as searching for ideas, evaluat­ing ideas, and postponing ideas. Both activities enable the instructor to assume the role of intervener in the students' writing processes, rather than evaluator of the text produced. In drawing The attention of the student write to both product and process, "Profiling" and "Pause Analysis" help them develop an awareness of the relation-ship between ideas in English expository text and the thinking pro­cesses that writers engage in while drafting such text.

  4. Profiles for voltage-activated currents are multiphasic, not curvilinear

    CERN Document Server

    Nissen, Per

    2016-01-01

    Data for voltage-activation of a potassium channel (Matulef et al. Proc Natl Acad Sci USA 110: 17886-17891. 2013) were, as conventionally done, fitted by the authors by a Boltzmann function, i.e. by a curvilinear profile. Reanalysis of the data reveals however that this interpretation must be rejected in favor of a multiphasic profile, a series of straight lines separated by discontinuous transitions, quite often in the form of noncontiguities (jumps). In contrast to the generally very poor fits to the Boltzmann profiles, the fits to multiphasic profiles are very good. (For the four replicates, the average deviations from the Boltzmann curves were 10- to 100-fold larger than the deviations from the multiphasic profiles.) The difference in the median values was statistically highly significant, P<0.001 in most cases. For the mean values the deviations from the Boltzmann curve were 20-fold larger than the deviations from the multiphasic profile, and the difference in the median values was also highly signifi...

  5. Active Tectonics Revealed by River Profiles along the Puqu Fault

    OpenAIRE

    Ping Lu,; Yu Shang

    2015-01-01

    The Puqu Fault is situated in Southern Tibet. It is influenced by the eastward extrusion of Northern Tibet and carries the clockwise rotation followed by the southward extrusion. Thus, the Puqu Fault is bounded by the principal dynamic zones and the tectonic evolution remains active alongside. This study intends to understand the tectonic activity in the Puqu Fault Region from the river profiles obtained from the remotely sensed satellite imagery. A medium resolution Digital Elevation Model (...

  6. Surface activity, lipid profiles and their implications in cervical cancer.

    Directory of Open Access Journals (Sweden)

    Preetha A

    2005-01-01

    Full Text Available Background: The profiles of lipids in normal and cancerous tissues may differ revealing information about cancer development and progression. Lipids being surface active, changes in lipid profiles can manifest as altered surface activity profiles. Langmuir monolayers offer a convenient model for evaluating surface activity of biological membranes. Aims: The aims of this study were to quantify phospholipids and their effects on surface activity of normal and cancerous human cervical tissues as well as to evaluate the role of phosphatidylcholine (PC and sphingomyelin (SM in cervical cancer using Langmuir monolayers. Methods and Materials: Lipid quantification was done using thin layer chromatography and phosphorus assay. Surface activity was evaluated using Langmuir monolayers. Monolayers were formed on the surface of deionized water by spreading tissue organic phase corresponding to 1 mg of tissue and studying their surface pressure-area isotherms at body temperature. The PC and SM contents of cancerous human cervical tissues were higher than those of the normal human cervical tissues. Role of PC and SM were evaluated by adding varying amounts of these lipids to normal cervical pooled organic phase. Statistical analysis: Student′s t-test (p < 0.05 and one-way analysis of variance (ANOVA was used. Results: Our results reveals that the phosphatidylglycerol level in cancerous cervical tissue was nearly five folds higher than that in normal cervical tissue. Also PC and sphingomyelin SM were found to be the major phospholipid components in cancerous and normal cervical tissues respectively. The addition of either 1.5 µg DPPC or 0.5 µg SM /mg of tissue to the normal organic phase changed its surface activity profile to that of the cancerous tissues. Statistically significant surface activity parameters showed that PC and SM have remarkable roles in shifting the normal cervical lipophilic surface activity towards that of cancerous lipophilic

  7. Graphical Turbulence Guidance - Composite

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Forecast turbulence hazards identified by the Graphical Turbulence Guidance algorithm. The Graphical Turbulence Guidance product depicts mid-level and upper-level...

  8. Chemoproteomic profiling of host and pathogen enzymes active in cholera

    Science.gov (United States)

    Hatzios, Stavroula K.; Hubbard, Troy; Sasabe, Jumpei; Munera, Diana; Clark, Lars; Bachovchin, Daniel A.; Qadri, Firdausi; Ryan, Edward T.; Davis, Brigid M.; Weerapana, Eranthie; Waldor, Matthew K.

    2016-01-01

    Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae proteases were consistently active in infected rabbits, and one, VC0157 (renamed IvaP), was also active in human cholera stool. Inactivation of IvaP influenced the activity of other secreted V. cholerae and rabbit enzymes in vivo, while genetic disruption of all four proteases increased the abundance and binding of an intestinal lectin—intelectin—to V. cholerae in infected rabbits. Intelectin also bound to other enteric bacterial pathogens, suggesting it may constitute a previously unrecognized mechanism of bacterial surveillance in the intestine that is inhibited by pathogen-secreted proteases. Our work demonstrates the power of activity-based proteomics to reveal host-pathogen enzymatic dialogue in an animal model of infection. PMID:26900865

  9. Graphics gems II

    CERN Document Server

    Arvo, James

    1991-01-01

    Graphics Gems II is a collection of articles shared by a diverse group of people that reflect ideas and approaches in graphics programming which can benefit other computer graphics programmers.This volume presents techniques for doing well-known graphics operations faster or easier. The book contains chapters devoted to topics on two-dimensional and three-dimensional geometry and algorithms, image processing, frame buffer techniques, and ray tracing techniques. The radiosity approach, matrix techniques, and numerical and programming techniques are likewise discussed.Graphics artists and comput

  10. The PC graphics handbook

    CERN Document Server

    Sanchez, Julio

    2003-01-01

    Part I - Graphics Fundamentals PC GRAPHICS OVERVIEW History and Evolution Short History of PC Video PS/2 Video Systems SuperVGA Graphics Coprocessors and Accelerators Graphics Applications State-of-the-Art in PC Graphics 3D Application Programming Interfaces POLYGONAL MODELING Vector and Raster Data Coordinate Systems Modeling with Polygons IMAGE TRANSFORMATIONS Matrix-based Representations Matrix Arithmetic 3D Transformations PROGRAMMING MATRIX TRANSFORMATIONS Numeric Data in Matrix Form Array Processing PROJECTIONS AND RENDERING Perspective The Rendering Pipeline LIGHTING AND SHADING Lightin

  11. Graphical Models with R

    DEFF Research Database (Denmark)

    Højsgaard, Søren; Edwards, David; Lauritzen, Steffen

    Graphical models in their modern form have been around since the late 1970s and appear today in many areas of the sciences. Along with the ongoing developments of graphical models, a number of different graphical modeling software programs have been written over the years. In recent years many...... of these software developments have taken place within the R community, either in the form of new packages or by providing an R ingerface to existing software. This book attempts to give the reader a gentle introduction to graphical modeling using R and the main features of some of these packages. In addition......, the book provides examples of how more advanced aspects of graphical modeling can be represented and handled within R. Topics covered in the seven chapters include graphical models for contingency tables, Gaussian and mixed graphical models, Bayesian networks and modeling high dimensional data...

  12. Design Archeology: Graphic Reconstructions of Kreuzberg, Berlin

    OpenAIRE

    2009-01-01

    Design Archeology combines communication design with archeological methods for evaluating material culture to produce new forms of graphic identity. It is a research methodology for examining cultural practices and activities of the moment in order to create a “snapshot” of a community’s identity from its material culture. Graphic Reconstructions of Kreuzberg Berlin is a graphic identity developed from ephemera (receipts, tickets, posters and flyers) collected from the streets of Kreuzberg, B...

  13. Mathematics and Computer Graphic Design Arts

    Institute of Scientific and Technical Information of China (English)

    徐亚非

    2001-01-01

    The relationship between arts and mathematics is very close, computer graphic design is based on digital methodology. The paper reveals the mathematical backgrounds behind graphic design by the example of computer-aided cubic modeling and mathematical exchange methodology. Furthermore, one can get incredible artistic effects if computer graphic designers pay more attention to the probability and use probable numbers and fractal operation in their design activities.Finally, the author also discusses the bidirections between arts and mathematics.

  14. A Survey on Graphical Programming Systems

    OpenAIRE

    Gurudatt Kulkarni; Sathyaraj. R

    2014-01-01

    Recently there has been an increasing interest in the use of graphics to help programming and understanding of computer systems. The Graphical Programming and Program Simulations are exciting areas of active computer science research that show the signs for improving the programming process. An array of different design methodologie s have arisen from research efforts and many graphical programming systems have been developed to address both general programming tasks and speci...

  15. Heart rate and activity profile for young female soccer players

    OpenAIRE

    2008-01-01

    The physical and physiological demands of high-level male soccer have been studied extensively, while few studies have investigated the demands placed on females during match-play, however, there is no information available about the heart rate and activity profile of young female soccer players during match play. Therefore, the aim of this study was to examine cardiovascular (heart-rates HR) and physical demands of young female soccer players during a match. Players were observed during a fr...

  16. Phytochemical profile, toxicity and antioxidant activity of Aloysia gratissima (Verbenaceae)

    OpenAIRE

    Zeni,Ana Lúcia B.; Albuquerque,Cláudia Almeida C. de; Filipe Gonçalves; Alexandra Latini; Tasca, Carla I.; Rossana Podestá; Pagliosa,Cristiane M.; DUARTE,FILIPE S.; Lima,Thereza C. M.; Marcelo Maraschin

    2013-01-01

    Aloysia gratissima (Gill. et Hook) Tronc. (Verbenaceae) is native to South America with folk therapeutic applications for a wide range of diseases. The polyphenolic and carotenoid profile, toxicity, and antioxidant activity of aqueous extract of Aloysia gratissima were investigated. HPLC analyses showed high amounts of ferulic acid, trans-cinnamic acid and p-coumaric acid, and also trans-β- carotene and lutein which fluctuated throughout the seasons. Furthermore, the extract investigated...

  17. Hidden Markov Models for the Activity Profile of Terrorist Groups

    CERN Document Server

    Raghavan, Vasanthan; Tartakovsky, Alexander G

    2012-01-01

    The main focus of this work is on developing models for the activity profile of a terrorist group, detecting sudden spurts and downfalls in this profile, and in general, tracking it over a period of time. Toward this goal, a d-state hidden Markov model (HMM) that captures the latent states underlying the dynamics of the group and thus its activity profile is developed. The simplest setting of d = 2 corresponds to the case where the dynamics are coarsely quantized as Active and Inactive, respectively. Two strategies for spurt detection and tracking are developed here: a model-independent strategy that uses the exponential weighted moving-average (EWMA) filter to track the strength of the group as measured by the number of attacks perpetrated by it, and a state estimation strategy that exploits the underlying HMM structure. The EWMA strategy is robust to modeling uncertainties and errors, and tracks persistent changes (changes that last for a sufficiently long duration) in the strength of the group. On the othe...

  18. Interplanetary Lyman $\\alpha$ line profiles: variations with solar activity cycle

    CERN Document Server

    Quemerais, E; Bertaux, J L; Koutroumpa, D; Clarke, J; Kyrola, E; Schmidt, W; Qu\\'emerais, Eric; Lallement, Rosine; Bertaux, Jean-Loup; Koutroumpa, Dimitra; Clarke, John; Kyrola, Erkki; Schmidt, Walter

    2006-01-01

    Interplanetary Lyman alpha line profiles are derived from the SWAN H cell data measurements. The measurements cover a 6-year period from solar minimum (1996) to after the solar maximum of 2001. This allows us to study the variations of the line profiles with solar activity. These line profiles were used to derive line shifts and line widths in the interplanetary medium for various angles of the LOS with the interstellar flow direction. The SWAN data results were then compared to an interplanetary background upwind spectrum obtained by STIS/HST in March 2001. We find that the LOS upwind velocity associated with the mean line shift of the IP \\lya line varies from 25.7 km/s to 21.4 km/s from solar minimum to solar maximum. Most of this change is linked with variations in the radiation pressure. LOS kinetic temperatures derived from IP line widths do not vary monotonically with the upwind angle of the LOS. This is not compatible with calculations of IP line profiles based on hot model distributions of interplanet...

  19. Phytochemical profile, toxicity and antioxidant activity of Aloysia gratissima (Verbenaceae

    Directory of Open Access Journals (Sweden)

    Ana Lúcia B. Zeni

    2013-01-01

    Full Text Available Aloysia gratissima (Gill. et Hook Tronc. (Verbenaceae is native to South America with folk therapeutic applications for a wide range of diseases. The polyphenolic and carotenoid profile, toxicity, and antioxidant activity of aqueous extract of Aloysia gratissima were investigated. HPLC analyses showed high amounts of ferulic acid, trans-cinnamic acid and p-coumaric acid, and also trans-β- carotene and lutein which fluctuated throughout the seasons. Furthermore, the extract investigated not only exerted antioxidant activity but also inhibited lipid peroxidation. Toxicity was achieved only at the highest dose tested. Therefore, A. gratissima is a potential species for medicinal purposes.

  20. Phenotype microarray profiling of the antibacterial activity of red cabbage

    Directory of Open Access Journals (Sweden)

    Hafidh RR

    2012-06-01

    Full Text Available Background: Functional food can be a potent source of wide array of biocomonents with antimicrobial activity. We investigated the antibacterial activity of red cabbage (RC extract on Gram negative and positive ATCC strains. Most intersting, we, for the first time, explored and analysed the complete phenotypic profile of RC-treated bacteria using Omnilog Phenotype Microarray. Results: This study revealed that the phenotype microarray (PM screen was a valuable tool in the search for compounds and their antibacterial mechanisms that can inhibit bacterial growth by affecting certain metabolic pathways. It was shown that RC exerted remarkable antibacterial effect on S. aureus and E. coli bacteria, and PM showed a wide range phenotypic profile of the exerted RC antibacterial activity. RC targeted the peptide, carbon, nutriontional assembly, and sulfur metbolic pathways altogether. The peptidoglycan synthesis pathway was inferred to be targeted by RC extract at a metabolic point different from other available cell wall-targeting drugs; these could be hot targets for the discovery of new therapy for many problematic microbes.Conclusions: Taken together, the phenotype microarray for functional food and medicinal plants can be a very useful tool for profiling their antimicrobial activity. Moreover, extracts of functional food can exert antibacterial activity by hitting a wide range of metabolic pathways, at the same time leading to very difficult condition for bacteria to rapidly develop resistance. Therefore, using functional foods or medicinal plants as such, or as extracts, can be superior on mono-targeting antibiotics if the optimal concentrations and conditions of these functional foods were sought.

  1. Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS

    NARCIS (Netherlands)

    Li, N.; Kuo, C.L.; Paniagua, G.; Elst, H. van den; Verdoes, M.; Willems, L.I.; Linden, W.A. van der; Ruben, M.; Genderen, E. van; Gubbens, J.; Wezel, G.P. van; Overkleeft, H.S.; Florea, B.I.

    2013-01-01

    Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site

  2. Universal Temporal Profile of Replication Origin Activation in Eukaryotes

    Science.gov (United States)

    Goldar, Arach

    2011-03-01

    The complete and faithful transmission of eukaryotic genome to daughter cells involves the timely duplication of mother cell's DNA. DNA replication starts at multiple chromosomal positions called replication origin. From each activated replication origin two replication forks progress in opposite direction and duplicate the mother cell's DNA. While it is widely accepted that in eukaryotic organisms replication origins are activated in a stochastic manner, little is known on the sources of the observed stochasticity. It is often associated to the population variability to enter S phase. We extract from a growing Saccharomyces cerevisiae population the average rate of origin activation in a single cell by combining single molecule measurements and a numerical deconvolution technique. We show that the temporal profile of the rate of origin activation in a single cell is similar to the one extracted from a replicating cell population. Taking into account this observation we exclude the population variability as the origin of observed stochasticity in origin activation. We confirm that the rate of origin activation increases in the early stage of S phase and decreases at the latter stage. The population average activation rate extracted from single molecule analysis is in prefect accordance with the activation rate extracted from published micro-array data, confirming therefore the homogeneity and genome scale invariance of dynamic of replication process. All these observations point toward a possible role of replication fork to control the rate of origin activation.

  3. Phenolic profile and antioxidant activity in selected seeds and sprouts.

    Science.gov (United States)

    Pająk, Paulina; Socha, Robert; Gałkowska, Dorota; Rożnowski, Jacek; Fortuna, Teresa

    2014-01-15

    The aim of this study was to investigate the effect of germination on the phenolic acids and flavonoids profile, as well as antioxidant activity (AA), in selected edible seeds of mung beans, radish, broccoli and sunflower. Germination increased the total phenolic (TP) and flavonoid (TF) levels, as well as the AA of the seeds, and influenced the profile of free and bound phenolic compounds. Among the samples, mung bean was characterised by lowest levels of TP and TF, as well as AA, evaluated using ABTS, DPPH and FRAP assays. Sunflower and radish sprouts were the most rich in phenolic compounds. Insignificant amounts of free phenolic acids were found in the free phenolic acid fraction; alkaline hydrolysis of the seeds and sprouts extracts provided the majority of the phenolic acids. The amounts of free and bound flavonoids were inconsiderable both for seeds and sprouts.

  4. Intelligent Computer Graphics 2012

    CERN Document Server

    Miaoulis, Georgios

    2013-01-01

    In Computer Graphics, the use of intelligent techniques started more recently than in other research areas. However, during these last two decades, the use of intelligent Computer Graphics techniques is growing up year after year and more and more interesting techniques are presented in this area.   The purpose of this volume is to present current work of the Intelligent Computer Graphics community, a community growing up year after year. This volume is a kind of continuation of the previously published Springer volumes “Artificial Intelligence Techniques for Computer Graphics” (2008), “Intelligent Computer Graphics 2009” (2009), “Intelligent Computer Graphics 2010” (2010) and “Intelligent Computer Graphics 2011” (2011).   Usually, this kind of volume contains, every year, selected extended papers from the corresponding 3IA Conference of the year. However, the current volume is made from directly reviewed and selected papers, submitted for publication in the volume “Intelligent Computer Gr...

  5. Introduction to Graphical Modelling

    CERN Document Server

    Scutari, Marco

    2010-01-01

    The aim of this chapter is twofold. In the first part we will provide a brief overview of the mathematical and statistical foundations of graphical models, along with their fundamental properties, estimation and basic inference procedures. In particular we will develop Markov networks (also known as Markov random fields) and Bayesian networks, which comprise most past and current literature on graphical models. In the second part we will review some applications of graphical models in systems biology.

  6. Deterministic Graphical Games Revisited

    DEFF Research Database (Denmark)

    Andersson, Daniel; Hansen, Kristoffer Arnsfelt; Miltersen, Peter Bro

    2008-01-01

    We revisit the deterministic graphical games of Washburn. A deterministic graphical game can be described as a simple stochastic game (a notion due to Anne Condon), except that we allow arbitrary real payoffs but disallow moves of chance. We study the complexity of solving deterministic graphical...... games and obtain an almost-linear time comparison-based algorithm for computing an equilibrium of such a game. The existence of a linear time comparison-based algorithm remains an open problem....

  7. Graphical Models with R

    CERN Document Server

    Højsgaard, Søren; Lauritzen, Steffen

    2012-01-01

    Graphical models in their modern form have been around since the late 1970s and appear today in many areas of the sciences. Along with the ongoing developments of graphical models, a number of different graphical modeling software programs have been written over the years. In recent years many of these software developments have taken place within the R community, either in the form of new packages or by providing an R interface to existing software. This book attempts to give the reader a gentle introduction to graphical modeling using R and the main features of some of these packages. In add

  8. Calcium Activation Profile In Electrically Stimulated Intact Rat Heart Cells

    Science.gov (United States)

    Geerts, Hugo; Nuydens, Rony; Ver Donck, Luc; Nuyens, Roger; De Brabander, Marc; Borgers, Marcel

    1988-06-01

    Recent advances in fluorescent probe technology and image processing equipment have made available the measurement of calcium in living systems on a real-time basis. We present the use of the calcium indicator Fura-2 in intact normally stimulated rat heart cells for the spatial and dynamic measurement of the calcium excitation profile. After electric stimulation (1 Hz), the activation proceeds from the center of the myocyte toward the periphery. Within two frame times (80 ms), the whole cell is activated. The activation is slightly faster in the center of the cell than in the periphery. The mean recovery time is 200-400 ms. There is no difference along the cell's long axis. The effect of a beta-agonist and of a calcium antagonist is described.

  9. Defining Dynamic Graphics by a Graphical Language

    Institute of Scientific and Technical Information of China (English)

    毛其昌; 戴汝为

    1991-01-01

    A graphical language which can be used for defining dynamic picture and applying control actions to it is defined with an expanded attributed grammar.Based on this a system is built for developing the presentation of application data of user interface.This system provides user interface designers with a friendly and high efficient programming environment.

  10. Graphical Modeling Language Tool

    NARCIS (Netherlands)

    Rumnit, M.

    2003-01-01

    The group of the faculty EE-Math-CS of the University of Twente is developing a graphical modeling language for specifying concurrency in software design. This graphical modeling language has a mathematical background based on the theorie of CSP. This language contains the power to create trustworth

  11. Phytochemical profile and biological activity of Juglans regia.

    Science.gov (United States)

    Panth, Nisha; Paudel, Keshav Raj; Karki, Rajendra

    2016-09-01

    Juglans regia Linn. (Juglandaceae), popularly known as English or Persian walnut, is a valuable medicinal plant with a potency to cure various diseases in traditional medicine. Since ancient time, different local ethnic groups have used various part of J. regia for a wide array of ailments including helminthiasis, diarrhea, sinusitis, stomach ache, arthritis, asthma, eczema, scrofula, skin disorders, diabetes mellitus, anorexia, thyroid dysfunction, cancer and infectious diseases. Biological activities of J. regia have been reported in several peer review journals and scientific attention is increasing. The present review attempts to provide comprehensive information on plant description, ethnobotanical use, toxicity, phytochemical profile, pharmacology, clinical studies and current research prospective of the J. regia. Currently, there is an immense interest on isolation/identification of active constituents from walnut and screening those active compounds for pharmacological activities. In addition, researchers are performing clinical trials as well as screening various solvent extracts or fractions of J. regia in several animal diseases models to identify promising therapeutic benefits. In the present work, we review the latest information based on published scientific investigations of J. regia.

  12. Active Tectonics Revealed by River Profiles along the Puqu Fault

    Directory of Open Access Journals (Sweden)

    Ping Lu

    2015-04-01

    Full Text Available The Puqu Fault is situated in Southern Tibet. It is influenced by the eastward extrusion of Northern Tibet and carries the clockwise rotation followed by the southward extrusion. Thus, the Puqu Fault is bounded by the principal dynamic zones and the tectonic evolution remains active alongside. This study intends to understand the tectonic activity in the Puqu Fault Region from the river profiles obtained from the remotely sensed satellite imagery. A medium resolution Digital Elevation Model (DEM, 20 m was generated from an Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER stereo pair of images and the stream network in this region was extracted from this DEM. The indices of slope and drainage area were subsequently calculated from this ASTER DEM. Based on the stream power law, the area-slope plots of the streams were delineated to derive the indices of channel concavity and steepness, which are closely related to tectonic activity. The results show the active tectonics varying significantly along the Puqu Fault, although the potential influence of glaciations may exist. These results are expected to be useful for a better understanding of tectonic evolution in Southeastern Tibet.

  13. Perception in statistical graphics

    Science.gov (United States)

    VanderPlas, Susan Ruth

    There has been quite a bit of research on statistical graphics and visualization, generally focused on new types of graphics, new software to create graphics, interactivity, and usability studies. Our ability to interpret and use statistical graphics hinges on the interface between the graph itself and the brain that perceives and interprets it, and there is substantially less research on the interplay between graph, eye, brain, and mind than is sufficient to understand the nature of these relationships. The goal of the work presented here is to further explore the interplay between a static graph, the translation of that graph from paper to mental representation (the journey from eye to brain), and the mental processes that operate on that graph once it is transferred into memory (mind). Understanding the perception of statistical graphics should allow researchers to create more effective graphs which produce fewer distortions and viewer errors while reducing the cognitive load necessary to understand the information presented in the graph. Taken together, these experiments should lay a foundation for exploring the perception of statistical graphics. There has been considerable research into the accuracy of numerical judgments viewers make from graphs, and these studies are useful, but it is more effective to understand how errors in these judgments occur so that the root cause of the error can be addressed directly. Understanding how visual reasoning relates to the ability to make judgments from graphs allows us to tailor graphics to particular target audiences. In addition, understanding the hierarchy of salient features in statistical graphics allows us to clearly communicate the important message from data or statistical models by constructing graphics which are designed specifically for the perceptual system.

  14. A Survey on Graphical Programming Systems

    Directory of Open Access Journals (Sweden)

    Gurudatt Kulkarni

    2014-04-01

    Full Text Available Recently there has been an increasing interest in the use of graphics to help programming and understanding of computer systems. The Graphical Programming and Program Simulations are exciting areas of active computer science research that show the signs for improving the programming process. An array of different design methodologie s have arisen from research efforts and many graphical programming systems have been developed to address both general programming tasks and specific application areas such as physical simulation and user interface design. This paper presents a survey of t he field of graphical programming languages starting with a historical overview of some of pioneering efforts in the field. In addition this paper also presents different classifications of graphical programming languages.

  15. Active flow control for a NACA-0012 Profile: Part II

    Science.gov (United States)

    Oualli, H.; Makadem, M.; Ouchene, H.; Ferfouri, A.; Bouabdallah, A.; Gad-El-Hak, M.

    2016-11-01

    Active flow control is applied to a NACA-0012 profile. The experiments are conducted in a wind tunnel. Using a high-resolution visible-light camera and tomography, flow visualizations are carried out. LES finite-volume 3D code is used to complement the physical experiments. The symmetric wing is clipped into two parts, and those parts extend and retract along the chord according to the same sinusoidal law we optimized last year for the same profile but clipped at an angle of 60 deg, instead of the original 90 deg. The Reynolds number range is extended to 500,000, thus covering the flying regimes of micro-UAVs, UAVs, as well as small aircraft. When the nascent cavity is open and the attack angle is 30 deg, the drag coefficient is increased by 1,300%, as compared to the uncontrolled case. However, when the cavity is covered and Re <=105 , a relatively small frequency, f <= 30 Hz, is required for the drag coefficient to drop to negative values. At the maximum Reynolds number, thrust is generated but only at much higher frequencies, 12 <= f <= 16 kHz.

  16. DEPTH CONTINUOUS HYDRAULIC CONDUCTIVITY PROFILING USING AN ACTIVE SOURCE PENETROMETER

    Science.gov (United States)

    Fitzgerald, M.; Elsworth, D.

    2009-12-01

    A method is developed to recover depth-continuous hydraulic conductivity profiles of an unconsolidated saturated aquifer using an active source penetrometer, the Hydraulic Profiling Tool (HPT). The tool yields estimates of K through continuous injection of fluid in the subsurface from a small port on the probe shaft while controlled measurements of net applied fluid pressure required to maintain a specified flow rate (typically 350 mL/min) are recorded. The tool gathers these data of flow rate and measured applied pressure during halted and constant-rate penetration (typically 2cm/sec) of the probe. The analysis is developed in two parts, first to explore the interplay between advective effects controlled by penetration rate and secondly flow volume effects controlled by the targeted flow rate. These two effects are analyzed through their respective influence on the measured applied pressure response in ΔP/σv’-Q/ΔP space, which shows a linear relationship for the flow rate to applied pressure response when Q/ΔP > 1 and when Q/ΔP 1. The analysis shows that penetration rate does not significantly influence the applied pressure response at the tested penetration rates (0 ≤ U(cm/s)≤ 4). The targeted applied flow rate does however influence the applied pressure response as flow rates less than ~300 mL/min show a scattering of the data in ΔP/σv’-Q/ΔP space, where above 300 mL/min the data begins to form a linear response. A targeted flow rate of QT = 400mL/min is suggested as a suitable flow rate based on this analysis. Measurements of hydraulic conductivity are then obtained for the HPT data through the derivation of an equation based on a recast form of Darcy’s law where considerations of the flow geometry as K = (QHPT/ΔP)(δw/πΦ). K profiles obtained for the HPT system are then compared against K profiles obtained from an independent method (PSU permeameter) and yield a good correlation between the two methods.

  17. Digital Raster Graphics

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A digital raster graphic (DRG) is a scanned image of a U.S. Geological Survey (USGS) topographic map. The scanned image includes all map collar information. The...

  18. Graphical Potential Games

    OpenAIRE

    Ortiz, Luis E.

    2015-01-01

    Potential games, originally introduced in the early 1990's by Lloyd Shapley, the 2012 Nobel Laureate in Economics, and his colleague Dov Monderer, are a very important class of models in game theory. They have special properties such as the existence of Nash equilibria in pure strategies. This note introduces graphical versions of potential games. Special cases of graphical potential games have already found applicability in many areas of science and engineering beyond economics, including ar...

  19. HEP graphics and visualization

    CERN Document Server

    Drevermann, Hans; CERN. Geneva

    1992-01-01

    The lectures will give an overview of the use of graphics in high-energy physics, i.e. for detector design, event representation and interactive analysis in 2D and 3D. An introduction to graphics packages (GKS, PHIGS, etc.) will be given, including discussion of the basic concepts of graphics programming. Emphasis is put on new ideas about graphical representation of events. Non-linear visualisation techniques, to improve the ease of understanding, will be described in detail. Physiological aspects, which play a role when using colours and when drawing mathematical objects like points and lines, are discussed. An analysis will be made of the power of graphics to represent very complex data in 2 and 3 dimensions, and the advantages of different representations will be compared.New techniques based on graphics are emerging today, such as multimedia or real-life pictures. Some are used in other domains of scientific research, as will be described and an overview of possible applications in our field will be give...

  20. Polyphenol profile and antioxidant activity of extracts from olive leaves

    Directory of Open Access Journals (Sweden)

    Svetla Yancheva

    2016-02-01

    Full Text Available The compounds, derivatives of olive leaves have a high antioxidant activity. The content of the total phenolic compounds (TPC, antioxidant activity (AOA and HPLC polyphenol profile of methanol extracts from the leaves of the olive cultivars Chondrolia Halkidiki, Kalamon, Koroneiki grown in the nursery (in vivo and in vitro plants of Chondrolia Halkidiki were compared. The results obtained for TPC varied between 9.2±0.5 mgGAE*gDW-1 and 16.4±0.5 mgGAE*gDW-1 . Antioxidant capacity was determined by four methods DPPH, ABTS, FRAP and CUPRAC. The highest results for TPC and AOA were achieved for the leaves of Chondrolia Halkidiki grown in vitro. A high correlation between the results gained from the TPC and AOA was established. Conducted HPLC analysis revealed the presence of 3,4- dihydroxybenzoic, caffeic, sinapic and ferulic acids and quercetin, hesperidin and luteolin and the quercetin glycosides rutin and hyperoside.

  1. Amateur boxing: activity profile of winners and losers.

    Science.gov (United States)

    Davis, Philip; Wittekind, Anna; Beneke, Ralph

    2013-01-01

    An activity profile of competitive 3 × 2-min novice-level amateur boxing was created based on video footage and postbout blood [La] in 32 male boxers (mean ± SD) age 19.3 ± 1.4 y, body mass 62.6 ± 4.1 kg. Winners landed 18 ± 11 more punches than losers by applying more lead-hand punches in round 1 (34.2 ± 10.9 vs 26.5 ± 9.4), total punches to the head (121.3 ± 10.2 vs 96.0 ± 9.8), and block and counterpunch combinations (2.8 ± 1.1 vs. 0.1 ± 0.2) over all 3 rounds and punching combinations (44.3 ± 6.4 vs 28.8 ± 6.7) in rounds 1 and 3 (all P < .05). In 16 boxers, peak postbout blood [La] was 11.8 ± 1.6 mmol/L irrespective of winning or losing. The results suggest that landing punches requires the ability to maintain a high frequency of attacking movements, in particular the lead-hand straight punch to the head together with punching combinations. Defensive movements must initiate a counterattack. Postbout blood [La] suggests that boxers must be able to tolerate a lactate production rate of 1.8 mmol · L-1 · min-1 and maintain skillful techniques at a sufficient activity rate.

  2. The activity profile of elite male amateur boxing.

    Science.gov (United States)

    Davis, Philip; Benson, Peter R; Pitty, James D; Connorton, Andrew J; Waldock, Robert

    2015-01-01

    An activity profile of competitive 3 × 3-min elite-level amateur boxing was created from video footage of 29 Olympic final and semifinal bouts in 39 male boxers (mean ± SD) age 25.1 ± 3.6 y, height 178.3 ± 10.4 cm, and body mass 69.7 ± 16.5 kg. Boxing at this level requires the ability to maintain an activity rate of ~1.4 actions/s, consisting of ~20 punches, ~2.5 defensive movements, and ~47 vertical hip movements, all per minute, over 3 subsequent rounds lasting ~200 s each. Winners had higher total punches landed (P = .041) and a lower ratio of punches thrown to landed (P = .027) than losers in round 3. The hook rear-hand landed was also higher for winners than losers in round 2 (P = .038) and round 3 (P = .016), and defensive movements were used less by winners (P = .036). However, the results suggest that technical discrimination between winners and losers is difficult; bout outcome may be more dependent on which punch is "lucky" enough to be scored by the judges or who appears to be dominant on the day. This study gives both boxers and coaches a good idea of where subelite boxers need to aim if they want to become among the best amateur boxers in the world.

  3. Open|SpeedShop Graphical User Interface Technology Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose to create a new graphical user interface (GUI) for an existing parallel application performance and profiling tool, Open|SpeedShop. The current GUI has...

  4. Publication-quality computer graphics

    Energy Technology Data Exchange (ETDEWEB)

    Slabbekorn, M.H.; Johnston, R.B. Jr.

    1981-01-01

    A user-friendly graphic software package is being used at Oak Ridge National Laboratory to produce publication-quality computer graphics. Close interaction between the graphic designer and computer programmer have helped to create a highly flexible computer graphics system. The programmer-oriented environment of computer graphics has been modified to allow the graphic designer freedom to exercise his expertise with lines, form, typography, and color. The resultant product rivals or surpasses that work previously done by hand. This presentation of computer-generated graphs, charts, diagrams, and line drawings clearly demonstrates the latitude and versatility of the software when directed by a graphic designer.

  5. Illustrating the Effect of pH on Enzyme Activity Using Gibbs Energy Profiles

    Science.gov (United States)

    Bearne, Stephen L.

    2014-01-01

    Gibbs energy profiles provide students with a visual representation of the energy changes that occur during enzyme catalysis, making such profiles useful as teaching and learning tools. Traditional kinetic topics, such as the effect of pH on enzyme activity, are often not discussed in terms of Gibbs energy profiles. Herein, the symbolism of Gibbs…

  6. Proteomic profile of Aspergillus flavus in response to water activity.

    Science.gov (United States)

    Zhang, Feng; Zhong, Hong; Han, Xiaoyun; Guo, Zhenni; Yang, Weiqiang; Liu, Yongfeng; Yang, Kunlong; Zhuang, Zhenhong; Wang, Shihua

    2015-03-01

    Aspergillus flavus, a common contaminant of crops and stored grains, can produce aflatoxins that are harmful to humans and other animals. Water activity (aw) is one of the key factors influencing both fungal growth and mycotoxin production. In this study, we used the isobaric tagging for relative and absolute quantitation (iTRAQ) technique to investigate the effect of aw on the proteomic profile of A. flavus. A total of 3566 proteins were identified, of which 837 were differentially expressed in response to variations in aw. Among these 837 proteins, 403 were over-expressed at 0.99 aw, whereas 434 proteins were over-expressed at 0.93 aw. According to Gene Ontology (GO) analysis, the secretion of extracellular hydrolases increased as aw was raised, suggesting that extracellular hydrolases may play a critical role in induction of aflatoxin biosynthesis. On the basis of Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) categorizations, we identified an exportin protein, KapK, that may down-regulate aflatoxin biosynthesis by changing the location of NirA. Finally, we considered the role of two osmotic stress-related proteins (Sln1 and Glo1) in the Hog1 pathway and investigated the expression patterns of proteins related to aflatoxin biosynthesis. The data uncovered in this study are critical for understanding the effect of water stress on toxin production and for the development of strategies to control toxin contamination of agricultural products.

  7. Diverting activities at Sellafield. [Use of remote technology and computer graphics for mock-up of diversion of raffinate feed pipe

    Energy Technology Data Exchange (ETDEWEB)

    1989-12-01

    Remote technology is being used to divert a raffinate feed pipe at the Sellafield fuel reprocessing plant in the UK. Extensive use is being made by the contractor of computer graphics and a full-scale mock-up. (author).

  8. Pharmacokinetic profiles of the active metamizole metabolites in healthy horses.

    Science.gov (United States)

    Giorgi, M; Aupanun, S; Lee, H-K; Poapolathep, A; Rychshanova, R; Vullo, C; Faillace, V; Laus, F

    2017-04-01

    Metamizole (MT) is an analgesic and antipyretic drug labelled for use in humans, horses, cattle, swine and dogs. MT is rapidly hydrolysed to the active primary metabolite 4-methylaminoantipyrine (MAA). MAA is formed in much larger amounts compared with other minor metabolites. Among the other secondary metabolites, 4-aminoantipyrine (AA) is also relatively active. The aim of this research was to evaluate the pharmacokinetic profiles of MAA and AA after dose of 25 mg/kg MT by intravenous (i.v.) and intramuscular (i.m.) routes in healthy horses. Six horses were randomly allocated to two equally sized treatment groups according to a 2 × 2 crossover study design. Blood was collected at predetermined times within 24 h, and plasma was analysed by a validated HPLC-UV method. No behavioural changes or alterations in health parameters were observed in the i.v. or i.m. groups of animals during or after (up to 7 days) drug administration. Plasma concentrations of MAA after i.v. and i.m. administrations of MT were detectable from 5 min to 10 h in all the horses. Plasma concentrations of AA were detectable in the same range of time, but in smaller amounts. Maximum concentration (Cmax ), time to maximum concentration (Tmax ) and AUMC0-last of MAA were statistically different between the i.v. and i.m. groups. The AUCIM /AUCIV ratio of MAA was 1.06. In contrast, AUC0-last of AA was statistically different between the groups (P < 0.05) with an AUCIM /AUCIV ratio of 0.54. This study suggested that the differences in the MAA and AA plasma concentrations found after i.m. and i.v. administrations of MT might have minor consequences on the pharmacodynamics of the drug.

  9. Mathematical structures for computer graphics

    CERN Document Server

    Janke, Steven J

    2014-01-01

    A comprehensive exploration of the mathematics behind the modeling and rendering of computer graphics scenes Mathematical Structures for Computer Graphics presents an accessible and intuitive approach to the mathematical ideas and techniques necessary for two- and three-dimensional computer graphics. Focusing on the significant mathematical results, the book establishes key algorithms used to build complex graphics scenes. Written for readers with various levels of mathematical background, the book develops a solid foundation for graphics techniques and fills in relevant grap

  10. Introduction to regression graphics

    CERN Document Server

    Cook, R Dennis

    2009-01-01

    Covers the use of dynamic and interactive computer graphics in linear regression analysis, focusing on analytical graphics. Features new techniques like plot rotation. The authors have composed their own regression code, using Xlisp-Stat language called R-code, which is a nearly complete system for linear regression analysis and can be utilized as the main computer program in a linear regression course. The accompanying disks, for both Macintosh and Windows computers, contain the R-code and Xlisp-Stat. An Instructor's Manual presenting detailed solutions to all the problems in the book is ava

  11. Raster graphics display library

    Science.gov (United States)

    Grimsrud, Anders; Stephenson, Michael B.

    1987-01-01

    The Raster Graphics Display Library (RGDL) is a high level subroutine package that give the advanced raster graphics display capabilities needed. The RGDL uses FORTRAN source code routines to build subroutines modular enough to use as stand-alone routines in a black box type of environment. Six examples are presented which will teach the use of RGDL in the fastest, most complete way possible. Routines within the display library that are used to produce raster graphics are presented in alphabetical order, each on a separate page. Each user-callable routine is described by function and calling parameters. All common blocks that are used in the display library are listed and the use of each variable within each common block is discussed. A reference on the include files that are necessary to compile the display library is contained. Each include file and its purpose are listed. The link map for MOVIE.BYU version 6, a general purpose computer graphics display system that uses RGDL software, is also contained.

  12. Comics & Graphic Novels

    Science.gov (United States)

    Cleaver, Samantha

    2008-01-01

    Not so many years ago, comic books in school were considered the enemy. Students caught sneaking comics between the pages of bulky--and less engaging--textbooks were likely sent to the principal. Today, however, comics, including classics such as "Superman" but also their generally more complex, nuanced cousins, graphic novels, are not only…

  13. Graphics Conference Calendar

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    1. The 13th International Conference in Central Europe on Computer Graphics, Visualization and Computer Vision'2005, University of West Bohemia, Campus-Bory Plzen (very close to Prague, the capital of the Czech Republic)Czech Republic, January 31 - February 4, 2005. http://wscg.zcu.cz, skala@kiv.zcu.cz

  14. Chemical profiling of Centella asiatica under different extraction solvents and its antibacterial activity, antioxidant activity

    Directory of Open Access Journals (Sweden)

    Supawan Rattanakom

    2015-12-01

    Full Text Available Centella asiatica (L urban, synonym Hydrocotyle asiatica, is found almost all over the world. This plant is famous in Ayurvedic medicine and used in the management of central nervous system, skin and gastrointestinal disorder. Thus this research had been done to evaluate the effect of solvent extraction (Ethanol, Chloroform and Hexane of C. asiatica on chemical profile, antioxidant activity and antibacterial activity against some foodborne pathogens. The result showed that all solvents (ethanol, chloroform and hexane used in extraction showed antibacterial activity against Salmonella enterica Typhimurium U302, S. enterica Enteritidis, S. enterica 4,5,12:I human (US clone, Bacillus cereus and B. subtilis at 50mg/ml concentration. In antioxidant part, ethanolic extract gave highest phenolic content and FRAP value. The results also showed that different extraction solvent gave different chemical profile. Hexane extract C. asiatica showed lowest in both antibacterial and antioxidant activity. Ethanolic and chloroform extract of C. asiatica showed promising potential in both antibacterial and antioxidant activity.

  15. Graphic Design for the Real World?

    DEFF Research Database (Denmark)

    Elisabeth Bichler, Katrine; Beier, Sofie

    2016-01-01

    This article examines graphic design’s role within design activism. It outlines design activism in general and its relation to commercial design culture in a consumerist economy. Thereafter it discusses persuasive tendencies in graphic design and questions if its current contribution to design...... activism is limited to its predominant narrow role of persuading for “the good cause.” To illustrate the hypothesis that such a persuasive approach lacks activist potential and thus social impact, cases that represent traditional graphic-design activism are compared to alternative approaches...... with an informative rather than persuasive character. The latter cases exemplify how information design can challenge the status quo and range from conventional leaflets to interactive tools and data visualizations. The discussion explores how these cases work as a non-commercial service to its audience, rather than...

  16. Graphics gems V (Macintosh version)

    CERN Document Server

    Paeth, Alan W

    1995-01-01

    Graphics Gems V is the newest volume in The Graphics Gems Series. It is intended to provide the graphics community with a set of practical tools for implementing new ideas and techniques, and to offer working solutions to real programming problems. These tools are written by a wide variety of graphics programmers from industry, academia, and research. The books in the series have become essential, time-saving tools for many programmers.Latest collection of graphics tips in The Graphics Gems Series written by the leading programmers in the field.Contains over 50 new gems displaying some of t

  17. Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions.

    Science.gov (United States)

    Ismail, Hanafy M; O'Neill, Paul M; Hong, David W; Finn, Robert D; Henderson, Colin J; Wright, Aaron T; Cravatt, Benjamin F; Hemingway, Janet; Paine, Mark J I

    2013-12-03

    Pyrethroid insecticides are used to control diseases spread by arthropods. We have developed a suite of pyrethroid mimetic activity-based probes (PyABPs) to selectively label and identify P450s associated with pyrethroid metabolism. The probes were screened against pyrethroid-metabolizing and nonmetabolizing mosquito P450s, as well as rodent microsomes, to measure labeling specificity, plus cytochrome P450 oxidoreductase and b5 knockout mouse livers to validate P450 activation and establish the role for b5 in probe activation. Using PyABPs, we were able to profile active enzymes in rat liver microsomes and identify pyrethroid-metabolizing enzymes in the target tissue. These included P450s as well as related detoxification enzymes, notably UDP-glucuronosyltransferases, suggesting a network of associated pyrethroid-metabolizing enzymes, or "pyrethrome." Considering the central role P450s play in metabolizing insecticides, we anticipate that PyABPs will aid in the identification and profiling of P450s associated with insecticide pharmacology in a wide range of species, improving understanding of P450-insecticide interactions and aiding the development of unique tools for disease control.

  18. Pyrethroid Activity-Based Probes for Profiling Cytochrome P450 Activities Associated with Insecticide Interactions

    Energy Technology Data Exchange (ETDEWEB)

    Ismail, Hanafy M.; O' Neill, Paul M.; Hong, David; Finn, Robert; Henderson, Colin; Wright, Aaron T.; Cravatt, Benjamin; Hemingway, Janet; Paine, Mark J.

    2014-01-18

    Pyrethroid insecticides are used to control a diverse spectrum of diseases spread by arthropods. We have developed a suite of pyrethroid mimetic activity based probes (PyABPs) to selectively label and identify P450s associated with pyrethroid metabolism. The probes were screened against pyrethroid metabolizing and non-metabolizing mosquito P450s, as well as rodent microsomes to measure labeling specificity, plus CPR and b5 knockout mouse livers to validate P450 activation and establish the role for b5 in probe activation. Using a deltamethrin mimetic PyABP we were able to profile active enzymes in rat liver microsomes and identify pyrethroid metabolizing enzymes in the target tissue. The most reactive enzyme was a P450, CYP2C11, which is known to metabolize deltamethrin. Furthermore, several other pyrethroid metabolizers were identified (CYPs 2C6, 3A4, 2C13 and 2D1) along with related detoxification enzymes, notably UDP-g’s 2B1 - 5, suggesting a network of associated pyrethroid metabolizing enzymes, or ‘pyrethrome’. Considering the central role that P450s play in metabolizing insecticides, we anticipate that PyABPs will aid the identification and profiling of P450s associated with insecticide pharmacology in a wide range of species, improving understanding of P450-insecticide interactions and aiding the development of new tools for disease control.

  19. Space Spurred Computer Graphics

    Science.gov (United States)

    1983-01-01

    Dicomed Corporation was asked by NASA in the early 1970s to develop processing capabilities for recording images sent from Mars by Viking spacecraft. The company produced a film recorder which increased the intensity levels and the capability for color recording. This development led to a strong technology base resulting in sophisticated computer graphics equipment. Dicomed systems are used to record CAD (computer aided design) and CAM (computer aided manufacturing) equipment, to update maps and produce computer generated animation.

  20. Graphic engine resource management

    Science.gov (United States)

    Bautin, Mikhail; Dwarakinath, Ashok; Chiueh, Tzi-cker

    2008-01-01

    Modern consumer-grade 3D graphic cards boast a computation/memory resource that can easily rival or even exceed that of standard desktop PCs. Although these cards are mainly designed for 3D gaming applications, their enormous computational power has attracted developers to port an increasing number of scientific computation programs to these cards, including matrix computation, collision detection, cryptography, database sorting, etc. As more and more applications run on 3D graphic cards, there is a need to allocate the computation/memory resource on these cards among the sharing applications more fairly and efficiently. In this paper, we describe the design, implementation and evaluation of a Graphic Processing Unit (GPU) scheduler based on Deficit Round Robin scheduling that successfully allocates to every process an equal share of the GPU time regardless of their demand. This scheduler, called GERM, estimates the execution time of each GPU command group based on dynamically collected statistics, and controls each process's GPU command production rate through its CPU scheduling priority. Measurements on the first GERM prototype show that this approach can keep the maximal GPU time consumption difference among concurrent GPU processes consistently below 5% for a variety of application mixes.

  1. Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis

    NARCIS (Netherlands)

    Bittencourt, M.L.F.; Ribeiro, Paulo R.; Franco, R.L.P.; Hilhorst, H.W.M.; Castro, de R.D.; Fernandez, L.G.

    2015-01-01

    The production of propolis by honeybees results from a selective collection of exudates from various plant species and present many potentialities in the pharmaceutical industry. The objective of this study was to investigate the chemical profile of Brazilian propolis, as well as their in vitro a

  2. Selecting Mangas and Graphic Novels

    Science.gov (United States)

    Nylund, Carol

    2007-01-01

    The decision to add graphic novels, and particularly the Japanese styled called manga, was one the author has debated for a long time. In this article, the author shares her experience when she purchased graphic novels and mangas to add to her library collection. She shares how graphic novels and mangas have revitalized the library.

  3. Activation profiles of opioid ligands in HEK cells expressing δ opioid receptors

    OpenAIRE

    Clark J; Demirci Hasan; Gharagozlou Parham; Lameh Jelveh

    2002-01-01

    Abstract Background The aim of the present study was to characterize the activation profiles of 15 opioid ligands in transfected human embryonic kidney cells expressing only δ opioid receptors. Activation profiles of most of these ligands at δ opioid receptors had not been previously characterized in vitro. Receptor activation was assessed by measuring the inhibition of forskolin-stimulated cAMP production. Results Naltrexone and nalorphine were classified as antagonists at δ opioid receptor....

  4. Relationships between physical education students' motivational profiles, enjoyment, state anxiety, and self-reported physical activity.

    Science.gov (United States)

    Yli-Piipari, Sami; Watt, Anthony; Jaakkola, Timo; Liukkonen, Jarmo; Nurmi, Jari-Erik

    2009-01-01

    The purpose of this study was to analyze motivational profiles based on the self-determination theory (Deci and Ryan, 2000) and how these profiles are related to physical education students' enjoyment, state anxiety, and physical activity. The participants, 429 sixth grade students (girls = 216; boys = 213) completed SMS, Sport Enjoyment Scale, PESAS, and Physical Activity Scale. Cluster analyses identified two motivational profiles: 1) the "High motivation profile", in which the students had high intrinsic and extrinsic motivation, and low levels of amotivation, and 2) the "Low motivation profile", in which the students had low intrinsic and extrinsic motivation, and low levels of amotivation. The students in the first cluster enjoyed physical education more and were physically more active. The results revealed that students may be motivated towards physical education lessons both intrinsically and extrinsically, and still experience enjoyment in physical education. Key pointsTWO MOTIVATIONAL PROFILES WERE REVEALED: 1) the "High motivation profile", in which the students had high intrinsic and extrinsic motivation, and low levels of amotivation, and 2) the "Low motivation profile", in which the students had low intrinsic and extrinsic motivation, and low levels of amotivation.The students in the first profile enjoyed physical education more and were physically more active than the students in the second profile.Moreover, the representatives of the "High motivation profile "experienced greater anxiety toward physical education than the representatives of the "Low motivation profile"These findings raised an interesting question whether students engaging in physical education benefit more from the presence of both self-determined and non-self-determined forms of motivation, or are the benefits higher if students are primarily self-determined?

  5. Graphics Gems III IBM version

    CERN Document Server

    Kirk, David

    1994-01-01

    This sequel to Graphics Gems (Academic Press, 1990), and Graphics Gems II (Academic Press, 1991) is a practical collection of computer graphics programming tools and techniques. Graphics Gems III contains a larger percentage of gems related to modeling and rendering, particularly lighting and shading. This new edition also covers image processing, numerical and programming techniques, modeling and transformations, 2D and 3D geometry and algorithms,ray tracing and radiosity, rendering, and more clever new tools and tricks for graphics programming. Volume III also includes a

  6. Direct and two-step activity-based profiling of proteases and glycosidases

    NARCIS (Netherlands)

    Willems, Lianne Irene

    2014-01-01

    Activity-based protein profiling provides a powerful approach for the monitoring of active enzyme populations in complex biological samples by making use of activity-based probes (ABPs), chemical probes that are designed to bind specifically to the active site of an enzyme (family). The research des

  7. RELATIONSHIPS BETWEEN PHYSICAL EDUCATION STUDENTS' MOTIVATIONAL PROFILES, ENJOYMENT, STATE ANXIETY, AND SELF-REPORTED PHYSICAL ACTIVITY

    Directory of Open Access Journals (Sweden)

    Sami Yli-Piipari

    2009-09-01

    Full Text Available The purpose of this study was to analyze motivational profiles based on the self-determination theory (Deci and Ryan, 2000 and how these profiles are related to physical education students' enjoyment, state anxiety, and physical activity. The participants, 429 sixth grade students (girls = 216; boys = 213 completed SMS, Sport Enjoyment Scale, PESAS, and Physical Activity Scale. Cluster analyses identified two motivational profiles: 1 the "High motivation profile", in which the students had high intrinsic and extrinsic motivation, and low levels of amotivation, and 2 the "Low motivation profile", in which the students had low intrinsic and extrinsic motivation, and low levels of amotivation. The students in the first cluster enjoyed physical education more and were physically more active. The results revealed that students may be motivated towards physical education lessons both intrinsically and extrinsically, and still experience enjoyment in physical education.

  8. What is Graphic Justice?

    Directory of Open Access Journals (Sweden)

    Thomas Giddens

    2016-12-01

    Full Text Available This article reproduces a poster presented at the Socio-Legal Studies ­Association annual conference, 5–7 April 2016 at Lancaster University, UK. The poster outlines the emerging study of the legal and jurisprudential dimensions of comics. Seeking to answer the question ‘what is graphic justice?’, the poster highlights the variety of potential topics, questions, concerns, issues, and intersections that the crossover between law and comics might encounter. A transcript of the poster’s text is provided for easier reuse, as well as a list of references and suggested readings.

  9. Application of Computer Graphics to Performance Studies of Missile Warheads

    Directory of Open Access Journals (Sweden)

    K. Rama Rao

    1991-01-01

    Full Text Available Intercept geometry of target aircraft and missiles play an important role in determining the effectiveness of the warhead. Factors such as fragment spatial distribution profile, damage capabilities, target and missile characteristics have been considered and visualised through computer graphics and optimum intercept intercept angles have been arrived. Computer graphics has proved to be an important tool to enhance perception and conceptual design capabilities in the design environment.

  10. Integration of rocket turbine design and analysis through computer graphics

    Science.gov (United States)

    Hsu, Wayne; Boynton, Jim

    1988-01-01

    An interactive approach with engineering computer graphics is used to integrate the design and analysis processes of a rocket engine turbine into a progressive and iterative design procedure. The processes are interconnected through pre- and postprocessors. The graphics are used to generate the blade profiles, their stacking, finite element generation, and analysis presentation through color graphics. Steps of the design process discussed include pitch-line design, axisymmetric hub-to-tip meridional design, and quasi-three-dimensional analysis. The viscous two- and three-dimensional analysis codes are executed after acceptable designs are achieved and estimates of initial losses are confirmed.

  11. Evaluation of in vivo biological activity profile of isoorientin.

    Science.gov (United States)

    Küpeli, Esra; Aslan, Mustafa; Gürbüz, Ilhan; Yesilada, Erdem

    2004-01-01

    Anti-nociceptive, anti-inflammatory and gastroprotective activities of the known C-glycosyl flavonoid, isoorientin, were studied in rats and mice. For the anti-nociceptive activity assessment the p-benzoquinone-induced writing test, for the anti-inflammatory activity the carrageenan-induced hind paw edema model in mice, and for the gastroprotective activity the EtOH-induced ulcerogenesis model in rats were used. Isoorientin was shown to possess significant anti-nociceptive and anti-inflammatory activities at 15 mg/kg and 30 mg/kg doses, without inducing any apparent acute toxicity as well as gastric damage. However, the compound did not possess any significant gastroprotective activity against EtOH-induced ulcerogenesis.

  12. Paneling "Matters" in Elementary Students' Graphic Narratives

    Science.gov (United States)

    Pantaleo, Sylvia

    2013-01-01

    During a 10-week classroom-based study, 20 fourth grade students participated in a number of interdependent activities that focused on developing their visual meaning-making skills and competencies. As well as reading, responding in writing to and discussing a selection of picturebooks, graphic novels, and magazines, the students created graphic…

  13. Intrusion Detection Architecture Utilizing Graphics Processors

    Directory of Open Access Journals (Sweden)

    Branislav Madoš

    2012-12-01

    Full Text Available With the thriving technology and the great increase in the usage of computer networks, the risk of having these network to be under attacks have been increased. Number of techniques have been created and designed to help in detecting and/or preventing such attacks. One common technique is the use of Intrusion Detection Systems (IDS. Today, number of open sources and commercial IDS are available to match enterprises requirements. However, the performance of these systems is still the main concern. This paper examines perceptions of intrusion detection architecture implementation, resulting from the use of graphics processor. It discusses recent research activities, developments and problems of operating systems security. Some exploratory evidence is presented that shows capabilities of using graphical processors and intrusion detection systems. The focus is on how knowledge experienced throughout the graphics processor inclusion has played out in the design of intrusion detection architecture that is seen as an opportunity to strengthen research expertise.

  14. Determining the neurotransmitter concentration profile at active synapses.

    Science.gov (United States)

    Scimemi, Annalisa; Beato, Marco

    2009-12-01

    Establishing the temporal and concentration profiles of neurotransmitters during synaptic release is an essential step towards understanding the basic properties of inter-neuronal communication in the central nervous system. A variety of ingenious attempts has been made to gain insights into this process, but the general inaccessibility of central synapses, intrinsic limitations of the techniques used, and natural variety of different synaptic environments have hindered a comprehensive description of this fundamental phenomenon. Here, we describe a number of experimental and theoretical findings that has been instrumental for advancing our knowledge of various features of neurotransmitter release, as well as newly developed tools that could overcome some limits of traditional pharmacological approaches and bring new impetus to the description of the complex mechanisms of synaptic transmission.

  15. Enzyme activity measurement via spectral evolution profiling and PARAFAC

    DEFF Research Database (Denmark)

    Baum, Andreas; Meyer, Anne S.; Garcia, Javier Lopez;

    2013-01-01

    The recent advances in multi-way analysis provide new solutions to traditional enzyme activity assessment. In the present study enzyme activity has been determined by monitoring spectral changes of substrates and products in real time. The method relies on measurement of distinct spectral fingerp...... parallel factor analysis (PARAFAC) for pectin lyase, glucose oxidase, and a cellulase preparation....

  16. Activity-Based Profiling of Retaining beta-Glucosidases : A Comparative Study

    NARCIS (Netherlands)

    Witte, Martin D.; Walvoort, Marthe T. C.; Li, Kah-Yee; Kallemeijn, Wouter W.; Donker-Koopman, Wilma E.; Boot, Rolf G.; Aerts, Johannes M. F. G.; Codee, Jeroen D. C.; van der Marel, Gijsbert A.; Overkleeft, Herman S.

    2011-01-01

    Activity-based protein profiling (ABPP) is a versatile strategy to report on enzyme activity in vitro, in situ, and in vivo. The development and use of ABPP tools and techniques has met with considerable success in monitoring physiological processes involving esterases and proteases. Activity-based

  17. Activity-Based Profiling of Retaining β-Glucosidases : A Comparative Study

    NARCIS (Netherlands)

    Witte, Martin D.; Walvoort, Marthe T.C.; Li, Kah-Yee; Kallemeijn, Wouter W.; Donker-Koopman, Wilma E.; Boot, Rolf G.; Aerts, Johannes M.F.G.; Codée, Jeroen D.C.; Marel, Gijsbert A. van der; Overkleeft, Herman S.

    2011-01-01

    Activity-based protein profiling (ABPP) is a versatile strategy to report on enzyme activity in vitro, in situ, and in vivo. The development and use of ABPP tools and techniques has met with considerable success in monitoring physiological processes involving esterases and proteases. Activity-based

  18. ANTIOXIDANT ACTIVITIES AND PHENOLIC PROFILE OF SIX MOROCCAN SELECTED HERBS

    Directory of Open Access Journals (Sweden)

    Madiha Bichra

    2013-02-01

    Full Text Available The present work evaluated the antioxidant capacity of six plants commonly used in traditional Moroccan medicine. The antioxidant capacity was estimated by DPPH test, ferrous ion chelating activity and ABTS test. As results, the highest antioxidant activities were found in Mentha suaveolens, Salvia officinalis and Mentha viridis. Different species showed significant differences in their total phenolic content (TPC. The highest level of phenolics was found in Salvia officinalis and the lowest in Pelargonium roseum. Linear correlation was found between TPC, especially the non-flavonoid content (NFC and the antioxidant activity. Qualitative and quantitative analyzes of major phenolics by reverse-phase high-performance liquid chromatography (RP-HPLC were also performed. On the basis of the obtained results, these studied medicinal herbs were found to serve as a potential source of natural antioxidants due to their richness in phenolic compounds and marked antioxidant activity.

  19. Detecting malicious activities with user-agent-based profiles

    OpenAIRE

    2015-01-01

    Hypertext transfer protocol (HTTP) has become the main protocol to carry out malicious activities. Attackers typically use HTTP for communication with command-and-control servers, click fraud, phishing and other malicious activities, as they can easily hide among the large amount of benign HTTP traffic. The user-agent (UA) field in the HTTP header carries information on the application, operating system (OS), device, and so on, and adversaries fake UA strings as a way to evade detection. Moti...

  20. Mapping graphic design practice & pedagogy

    OpenAIRE

    Corazzo, James; Raven, Darren

    2016-01-01

    Workshop Description Mapping graphic design pedagogy will explore the complex, expanding and fragmenting fields of graphic design through the process of visual mapping. This experimental, collaborative workshop will enable participants to conceive and develop useful frameworks for navigating the expanding arena of graphic design that has grown from its roots in professional practice and now come to include areas of ethical, political, socio-economic, cultural and critical design. For a...

  1. Graphics and visualization principles & algorithms

    CERN Document Server

    Theoharis, T; Platis, Nikolaos; Patrikalakis, Nicholas M

    2008-01-01

    Computer and engineering collections strong in applied graphics and analysis of visual data via computer will find Graphics & Visualization: Principles and Algorithms makes an excellent classroom text as well as supplemental reading. It integrates coverage of computer graphics and other visualization topics, from shadow geneeration and particle tracing to spatial subdivision and vector data visualization, and it provides a thorough review of literature from multiple experts, making for a comprehensive review essential to any advanced computer study.-California Bookw

  2. Graphical models for genetic analyses

    DEFF Research Database (Denmark)

    Lauritzen, Steffen Lilholt; Sheehan, Nuala A.

    2003-01-01

    This paper introduces graphical models as a natural environment in which to formulate and solve problems in genetics and related areas. Particular emphasis is given to the relationships among various local computation algorithms which have been developed within the hitherto mostly separate areas...... of graphical models and genetics. The potential of graphical models is explored and illustrated through a number of example applications where the genetic element is substantial or dominating....

  3. Use of focused ultrasonication in activity-based profiling of deubiquitinating enzymes in tissue.

    Science.gov (United States)

    Nanduri, Bindu; Shack, Leslie A; Rai, Aswathy N; Epperson, William B; Baumgartner, Wes; Schmidt, Ty B; Edelmann, Mariola J

    2016-12-15

    To develop a reproducible tissue lysis method that retains enzyme function for activity-based protein profiling, we compared four different methods to obtain protein extracts from bovine lung tissue: focused ultrasonication, standard sonication, mortar & pestle method, and homogenization combined with standard sonication. Focused ultrasonication and mortar & pestle methods were sufficiently effective for activity-based profiling of deubiquitinases in tissue, and focused ultrasonication also had the fastest processing time. We used focused-ultrasonicator for subsequent activity-based proteomic analysis of deubiquitinases to test the compatibility of this method in sample preparation for activity-based chemical proteomics.

  4. Synthesising Graphical Theories

    CERN Document Server

    Kissinger, Aleks

    2012-01-01

    In recent years, diagrammatic languages have been shown to be a powerful and expressive tool for reasoning about physical, logical, and semantic processes represented as morphisms in a monoidal category. In particular, categorical quantum mechanics, or "Quantum Picturalism", aims to turn concrete features of quantum theory into abstract structural properties, expressed in the form of diagrammatic identities. One way we search for these properties is to start with a concrete model (e.g. a set of linear maps or finite relations) and start composing generators into diagrams and looking for graphical identities. Naively, we could automate this procedure by enumerating all diagrams up to a given size and check for equalities, but this is intractable in practice because it produces far too many equations. Luckily, many of these identities are not primitive, but rather derivable from simpler ones. In 2010, Johansson, Dixon, and Bundy developed a technique called conjecture synthesis for automatically generating conj...

  5. Deterministic Graphical Games Revisited

    DEFF Research Database (Denmark)

    Andersson, Klas Olof Daniel; Hansen, Kristoffer Arnsfelt; Miltersen, Peter Bro

    2012-01-01

    Starting from Zermelo’s classical formal treatment of chess, we trace through history the analysis of two-player win/lose/draw games with perfect information and potentially infinite play. Such chess-like games have appeared in many different research communities, and methods for solving them......, such as retrograde analysis, have been rediscovered independently. We then revisit Washburn’s deterministic graphical games (DGGs), a natural generalization of chess-like games to arbitrary zero-sum payoffs. We study the complexity of solving DGGs and obtain an almost-linear time comparison-based algorithm...... for finding optimal strategies in such games. The existence of a linear time comparison-based algorithm remains an open problem....

  6. Computer graphics in engineering education

    CERN Document Server

    Rogers, David F

    2013-01-01

    Computer Graphics in Engineering Education discusses the use of Computer Aided Design (CAD) and Computer Aided Manufacturing (CAM) as an instructional material in engineering education. Each of the nine chapters of this book covers topics and cites examples that are relevant to the relationship of CAD-CAM with engineering education. The first chapter discusses the use of computer graphics in the U.S. Naval Academy, while Chapter 2 covers key issues in instructional computer graphics. This book then discusses low-cost computer graphics in engineering education. Chapter 4 discusses the uniform b

  7. Graphics shaders theory and practice

    CERN Document Server

    Bailey, Mike

    2011-01-01

    Graphics Shaders: Theory and Practice is intended for a second course in computer graphics at the undergraduate or graduate level, introducing shader programming in general, but focusing on the GLSL shading language. While teaching how to write programmable shaders, the authors also teach and reinforce the fundamentals of computer graphics. The second edition has been updated to incorporate changes in the OpenGL API (OpenGL 4.x and GLSL 4.x0) and also has a chapter on the new tessellation shaders, including many practical examples. The book starts with a quick review of the graphics pipeline,

  8. 3D for Graphic Designers

    CERN Document Server

    Connell, Ellery

    2011-01-01

    Helping graphic designers expand their 2D skills into the 3D space The trend in graphic design is towards 3D, with the demand for motion graphics, animation, photorealism, and interactivity rapidly increasing. And with the meteoric rise of iPads, smartphones, and other interactive devices, the design landscape is changing faster than ever.2D digital artists who need a quick and efficient way to join this brave new world will want 3D for Graphic Designers. Readers get hands-on basic training in working in the 3D space, including product design, industrial design and visualization, modeling, ani

  9. Anti-inflammatory activity and chemical profile of Galphimia glauca.

    Science.gov (United States)

    González-Cortazar, Manasés; Herrera-Ruiz, Maribel; Zamilpa, Alejandro; Jiménez-Ferrer, Enrique; Marquina, Silvia; Alvarez, Laura; Tortoriello, Jaime

    2014-01-01

    Galphimia glauca, commonly known as "flor de estrella", is a plant species used in Mexican traditional medicine for the treatment of different diseases that have an acute or chronic inflammatory process in common. Aerial parts of this plant contain nor-seco-triterpenoids with anxiolytic properties, which have been denominated galphimines. Other compounds identified in the plant are tetragalloyl-quinic acid, gallic acid, and quercetin, which are able to inhibit the bronchial obstruction induced by platelet-activating factor. The objective of this work was to evaluate the anti-inflammatory effect of crude extracts from G. glauca and, by means of bioguided chemical separation, to identify the compounds responsible for this pharmacological activity. n-Hexane, ethyl acetate, dichloromethane, and methanol extracts showed an important anti-inflammatory effect. Chemical separation of the active methanol extract allowed us to identify the nor-seco-triterpenes galphimine-A (1) and galphimine-E (3) as the anti-inflammatory principles. Analysis of structure-activity relationships evidenced that the presence of an oxygenated function in C6 is absolutely necessary to show activity. In this work, the isolation and structural elucidation of two new nor-seco-triterpenes denominated as galphimine-K (4) and galphimine-L (5), together with different alkanes, fatty acids, as well as three flavonoids (17-19), are described, to our knowledge for the first time, from Galphimia glauca.

  10. Garlic sprouting is associated with increased antioxidant activity and concomitant changes in the metabolite profile.

    Science.gov (United States)

    Zakarova, Alexandra; Seo, Ji Yeon; Kim, Hyang Yeon; Kim, Jeong Hwan; Shin, Jung-Hye; Cho, Kye Man; Lee, Choong Hwan; Kim, Jong-Sang

    2014-02-26

    Although garlic (Allium sativum) has been extensively studied for its health benefits, sprouted garlic has received little attention. We hypothesized that sprouting garlic would stimulate the production of various phytochemicals that improve health. Ethanolic extracts from garlic sprouted for different periods had variable antioxidant activities when assessed with in vitro assays, including the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay and the oxygen radical absorbance capacity assay. Extracts from garlic sprouted for 5 days had the highest antioxidant activity, whereas extracts from raw garlic had relatively low antioxidant activity. Furthermore, sprouting changed the metabolite profile of garlic: the metabolite profile of garlic sprouted for 5-6 days was distinct from the metabolite profile of garlic sprouted for 0-4 days, which is consistent with the finding that garlic sprouted for 5 days had the highest antioxidant activity. Therefore, sprouting may be a useful way to improve the antioxidant potential of garlic.

  11. Enzymatic activities and protein profile of latex from Calotropis procera.

    Science.gov (United States)

    Freitas, Cleverson Diniz T; Oliveira, Jefferson Soares; Miranda, Maria Raquel A; Macedo, Nívea Maria R; Sales, Maurício Pereira; Villas-Boas, Laurival A; Ramos, Márcio Viana

    2007-01-01

    The laticifer fluid of Calotropis procera is rich in proteins and there is evidence that they are involved in the pharmacological properties of the latex. However, not much is known about how the latex-containing proteins are produced or their functions. In this study, laticifer proteins of C. procera were pooled and examined by 1D and 2D electrophoresis, masses spectrometry (MALDI-TOF) and characterized in respect of proteolytic activity and oxidative enzymes. Soluble laticifer proteins were predominantly composed of basic proteins (PI>6.0) with molecular masses varying between 5 and 95 kDa. Proteins with a molecular mass of approximately 26,000 Da were more evident. Strong anti-oxidative activity of superoxide dismutase (EC 1.15.1.1) (1007.74+/-91.89 Ug(-1)DM) and, to a lesser extent ascorbate peroxidase (EC 1.11.1.1) (0.117(d)+/-0.013 microMol H(2)O(2)g(-1)min(-1)), were detected. However, catalase (EC 1.11.1.6) was absent. The strong proteolytic activities of laticifer proteins from C. procera were shown to be shared by at least four distinct cysteine proteinases (EC 3.4.22.16) that were isolated by gel filtration chromatography. Serine and metaloproteinases were not detected and aspartic proteinase activities were barely visible. Chitinases (EC 3.2.1.14) were also isolated in a chitin column and their activities quantified. The presence of these enzymatic activities in latex from C. procera may confirm their involvement in resistance to phytopathogens and insects, mainly in its leaves where the latex circulates abundantly.

  12. Activity profiling of papain-like cysteine proteases in plants

    NARCIS (Netherlands)

    Hoorn, van der R.A.L.; Leeuwenburgh, M.A.; Bogyo, M.; Joosten, M.H.A.J.; Peck, S.C.

    2004-01-01

    Transcriptomic and proteomic technologies are generating a wealth of data that are frequently used by scientists to predict the function of proteins based on their expression or presence. However, activity of many proteins, such as transcription factors, kinases, and proteases, depends on posttransl

  13. Dysregulation of Macrophage Activation Profiles by Engineered Nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Kodali, Vamsi; Littke, Matthew H.; Tilton, Susan C.; Teeguarden, Justin G.; Shi, Liang; Frevert, Charles W.; Wang, Wei; Pounds, Joel G.; Thrall, Brian D.

    2013-08-27

    Although the potential human health impacts from exposure to engineered nanoparticles (ENPs) are uncertain, past epidemiological studies have established correlations between exposure to ambient air pollution particulates and the incidence of pneumonia and lung infections. Using amorphous silica and superparamagnetic iron oxide (SPIO) as model high production volume ENPs, we examined how macrophage activation by bacterial lipopolysaccharide (LPS) or the lung pathogen Streptococcus pneumoniae is altered by ENP pretreatment. Neither silica nor SPIO treatment elicited direct cytotoxic or pro-inflammatory effects in bone marrow-derived macrophages. However, pretreatment of macrophages with SPIO caused extensive reprogramming of nearly 500 genes regulated in response to LPS challenge, hallmarked by exaggerated activation of oxidative stress response pathways and suppressed activation of both pro- and anti-inflammatory pathways. Silica pretreatment altered regulation of only 67 genes, but there was strong correlation with gene sets affected by SPIO. Macrophages exposed to SPIO displayed a phenotype suggesting an impaired ability to transition from an M1 to M2-like activation state, characterized by suppressed IL-10 induction, enhanced TNFα production, and diminished phagocytic activity toward S. pneumoniae. Studies in macrophages deficient in scavenger receptor A (SR-A) showed SR-A participates in cell uptake of both the ENPs and S. pneumonia and co-regulates the anti-inflammatory IL-10 pathway. Thus, mechanisms for dysregulation of innate immunity exist by virtue that common receptor recognition pathways are used by some ENPs and pathogenic bacteria, although the extent of transcriptional reprogramming of macrophage function depends on the physicochemical properties of the ENP after internalization. Our results also illustrate that biological effects of ENPs may be indirectly manifested only after challenging normal cell function. Finally, nanotoxicology screening

  14. Chilean prosopis mesocarp flour: phenolic profiling and antioxidant activity.

    Science.gov (United States)

    Schmeda-Hirschmann, Guillermo; Quispe, Cristina; Soriano, Maria Del Pilar C; Theoduloz, Cristina; Jiménez-Aspée, Felipe; Pérez, Maria Jorgelina; Cuello, Ana Soledad; Isla, Maria Inés

    2015-04-17

    In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82-2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  15. Chilean Prosopis Mesocarp Flour: Phenolic Profiling and Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Guillermo Schmeda-Hirschmann

    2015-04-01

    Full Text Available In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82–2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  16. Graphics Display of Foreign Scripts.

    Science.gov (United States)

    Abercrombie, John R.

    1987-01-01

    Describes Graphics Project for Foreign Language Learning at the University of Pennsylvania, which has developed ways of displaying foreign scripts on microcomputers. Character design on computer screens is explained; software for graphics, printing, and language instruction is discussed; and a text editor is described that corrects optically…

  17. Lipid, lipoprotein, and apolipoprotein profiles in active and sedentary men with tetraplegia

    NARCIS (Netherlands)

    Dallmeijer, A J; Hopman, M T; van der Woude, L H

    1997-01-01

    OBJECTIVE: To investigate whether the risk profile of coronary heart disease (CHD) is more favorable in physically active men with tetraplegia compared with sedentary men with tetraplegia. DESIGN: Using a cross-sectional design, the lipid and (apo)lipoprotein concentrations of 11 active and 13 seden

  18. Organosulphide profile and hydrogen sulphide-releasing activity of garlic fermented by Lactobacillus plantarum

    NARCIS (Netherlands)

    Tocmo, Restituto; Lai, Abigail Nianci; Wu, Yuchen; Liang, Dong; Fogliano, Vincenzo; Huang, Dejian

    2017-01-01

    Blanched and unblanched garlic were fermented using L. plantarum for investigation of organosulphide profiles, hydrogen sulphide-releasing activity, pH, titratable activity and microbial growth. Both raw and blanched garlic preparations allowed growth of L. plantarum with corresponding lowering of p

  19. Phenolic Profiles and Antioxidant Activity of Lotus Root Varieties

    Directory of Open Access Journals (Sweden)

    Yang Yi

    2016-06-01

    Full Text Available Lotus root attracts increasing attention mainly because of its phenolic compounds known as natural antioxidants. Its thirteen varieties were systematically analyzed on the content, distribution, composition and antioxidant activity of phenolic compounds for a better understanding of this aquatic vegetable. The respective mean contents of total phenolics in their flesh, peel and nodes were 1.81, 4.30 and 7.35 mg gallic acid equivalents (GAE/g fresh weight (FW, and those of total flavonoids were 3.35, 7.69 and 15.58 mg rutin equivalents/g FW. The phenolic composition determined by a high-performance liquid chromatography method varied significantly among varieties and parts. The phenolics of flesh were mainly composed of gallocatechin and catechin; those of peel and node were mainly composed of gallocatechin, gallic acid, catechin and epicatechin. The antioxidant activities of phenolic extracts in increasing order were flesh, peel and node; their mean concentrations for 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical were 46.00, 26.43 and 21.72 µg GAE/mL, and their mean values representing ferric reducing antioxidant power were 75.91, 87.66 and 100.43 µg Trolox equivalents/100 µg GAE, respectively. “Zoumayang”, “Baheou”, “No. 5 elian” and “Guixi Fuou” were the hierarchically clustered varieties with relatively higher phenolic content and stronger antioxidant activity as compared with the others. Especially, their nodes and peels are promising sources of antioxidants for human nutrition.

  20. Elite futsal refereeing: Activity profile and physiological demands

    DEFF Research Database (Denmark)

    Rebelo, António N.; Ascensão, António A.; Magalhães, José F.

    2011-01-01

    the game. Endurance capacity was determined with the Yo-Yo IE2. The number of activity changes was as high as 1,395 ± 218 (±SD). Total distance covered, high-intensity running (HIR), sprinting (SPR), and sideways running were 5.89 ± 0.56, 0.96 ± 0.29, 0.09 ± 0.07, and 0.91 ± 0.46 km, respectively....... The number of HIR and SPR bouts was 129 ± 41 and 9 ± 8, respectively, with a mean duration of ~1.4 seconds. Blood lactate content was 1.0 ± 0.3 and 1.5 ± 0.5 mM before and after the game. The amount of HIR performed during the match correlated significantly (r = 0.77; p Yo-Yo IE2 performance...

  1. Proteomic profiling of endorepellin angiostatic activity on human endothelial cells

    Directory of Open Access Journals (Sweden)

    Iozzo Renato V

    2008-02-01

    Full Text Available Abstract Background Endorepellin, the C-terminal domain V of the heparan sulfate proteoglycan perlecan, exhibits powerful and targeted anti-angiogenic activity on endothelial cells. To identify proteins involved with endorepellin anti-angiogenic action, we performed an extensive comparative proteomic analysis between vehicle- and endorepellin-treated human endothelial cells. Results Proteomic analysis of endorepellin influence on human umbilical vein endothelial cells identified five differentially expressed proteins, three of which (β-actin, calreticulin, and chaperonin/Hsp60 were down-regulated and two of which (vimentin and the β subunit of prolyl 4-hydroxylase also known as protein disulfide isomerase were up-regulated in response to endorepellin treatment—and associated with a fold change (endorepellin/control ≤ 0.75 and ≥ 2.00, and a statistically significant p-value as determined by Student's t test. Conclusion The proteins identified represent potential target areas involved with endorepellin anti-angiogenic mechanism of action. Further elucidation as such will ultimately provide useful in utilizing endorepellin as an anti-angiogenic therapy in humans.

  2. 78 FR 66698 - Pesticides; Repellency Awareness Graphic; Notice of Availability

    Science.gov (United States)

    2013-11-06

    ... presented the concept, draft guidance document, and draft graphic to OPP's federal advisory committee, the... protection activities for the prevention of vector- ] borne diseases such as West Nile virus and Lyme...

  3. Proteomic and activity profiles of ascorbate-glutathione cycle enzymes in germinating barley embryo

    DEFF Research Database (Denmark)

    Bønsager, Birgit Christine; Shahpiri, Azar; Finnie, Christine

    2010-01-01

    Enzymes involved in redox control are important during seed germination and seedling growth. Ascorbate-glutathione cycle enzymes in barley embryo extracts were monitored both by 2D-gel electrophoresis and activity measurements from 4 to 144 h post imbibition (PI). Strikingly different activity...... profiles were observed. No ascorbate peroxidase (APX) activity was present in mature seeds but activity was detected after 24 h PI and increased 14-fold up to 144 h PI. In contrast, dehydroascorbate reductase (DHAR) activity was present at 4 h PI and first decreased by 9-fold until 72 h PI followed by a 5...

  4. IAU Graphics Extension - Gnu C

    DEFF Research Database (Denmark)

    Denver, Troelz; Jørgensen, John Leif; Riis, Troels

    1999-01-01

    This document contains a description of the library GrxIau (former UtilVesa and UTILLE). The library acts as shell to the graphics commands used at the exercises at the course 50240 Image Analysis with Microcomputers....

  5. Digital Raster Graphics (DRG) Lambert

    Data.gov (United States)

    Kansas Data Access and Support Center — The Digital Raster Graphic-Lambert (DRG-Lam) is a raster image of a scanned USGS topographic map with the collar information clipped out, georeferenced to the...

  6. Graphic design of pinhole cameras

    Science.gov (United States)

    Edwards, H. B.; Chu, W. P.

    1979-01-01

    The paper describes a graphic technique for the analysis and optimization of pinhole size and focal length. The technique is based on the use of the transfer function of optical elements described by Scott (1959) to construct the transfer function of a circular pinhole camera. This transfer function is the response of a component or system to a pattern of lines having a sinusoidally varying radiance at varying spatial frequencies. Some specific examples of graphic design are presented.

  7. Automated Structure-Activity Relationship Mining: Connecting Chemical Structure to Biological Profiles.

    Science.gov (United States)

    Wawer, Mathias J; Jaramillo, David E; Dančík, Vlado; Fass, Daniel M; Haggarty, Stephen J; Shamji, Alykhan F; Wagner, Bridget K; Schreiber, Stuart L; Clemons, Paul A

    2014-06-01

    Understanding the structure-activity relationships (SARs) of small molecules is important for developing probes and novel therapeutic agents in chemical biology and drug discovery. Increasingly, multiplexed small-molecule profiling assays allow simultaneous measurement of many biological response parameters for the same compound (e.g., expression levels for many genes or binding constants against many proteins). Although such methods promise to capture SARs with high granularity, few computational methods are available to support SAR analyses of high-dimensional compound activity profiles. Many of these methods are not generally applicable or reduce the activity space to scalar summary statistics before establishing SARs. In this article, we present a versatile computational method that automatically extracts interpretable SAR rules from high-dimensional profiling data. The rules connect chemical structural features of compounds to patterns in their biological activity profiles. We applied our method to data from novel cell-based gene-expression and imaging assays collected on more than 30,000 small molecules. Based on the rules identified for this data set, we prioritized groups of compounds for further study, including a novel set of putative histone deacetylase inhibitors.

  8. Health profile for Danish adults with activity limitation and/or physical disability

    DEFF Research Database (Denmark)

    Johnsen, Nina Føns; Davidsen, Michael; Juel, Knud

    Presentation type: Pitch Health profile for Danish adults with activity limitation and/or physical disability Presenting author: Nina Føns Johnsen, nifj@si-folkesundhed.dk Authors: NF, Johnsen; M, Davidsen; SI, Michelsen; K, Juel Affiliation: National Institute of Public Health, University...... activity limitation. Similarly, the proportion of individuals with headache, psychological symptoms, daily smoking, physical inactivity, obesity and infrequent contact with family or friends was twice as high. Similar results were found for individuals with physical disabilities. Conclusions A smaller...

  9. Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles.

    OpenAIRE

    Carozzi, N B; Kramer, V C; Warren, G W; Evola, S; Koziel, M G

    1991-01-01

    A rapid analysis of Bacillus thuringiensis strains predictive of insecticidal activity was established by using polymerase chain reaction (PCR) technology. Primers specific to regions of high homology within genes encoding three major classes of B. thuringiensis crystal proteins were used to generate a PCR product profile characteristic of each insecticidal class. Predictions of insecticidal activity were made on the basis of the electrophoretic patterns of the PCR products. Included in the s...

  10. Statistical graphics: mapping the pathways of science.

    Science.gov (United States)

    Wainer, H; Velleman, P F

    2001-01-01

    This chapter traces the evolution of statistical graphics starting with its departure from the common noun structure of Cartesian determinism, through William Playfair's revolutionary grammatical shift to graphs as proper nouns, and alights on the modern conception of graph as an active participant in the scientific process of discovery. The ubiquitous availability of data, software, and cheap, high-powered, computing when coupled with the broad acceptance of the ideas in Tukey's 1977 treatise on exploratory data analysis has yielded a fundamental change in the way that the role of statistical graphics is thought of within science-as a dynamic partner and guide to the future rather than as a static monument to the discoveries of the past. We commemorate and illustrate this development while pointing readers to the new tools available and providing some indications of their potential.

  11. Reproductive activity in cows following parturition against values of certain parameters of metabolic profile

    Directory of Open Access Journals (Sweden)

    Otava Gabriel

    2006-01-01

    Full Text Available Data from literature indicate that there is a correlation between the values of reproductive parameters and the values of parameters of the metabolic profile, even though this cannot be confirmed in many cases. The objective of this work was to examine the connection between certain parameters of the metabolic profile and the restoration of reproductive activity in the postpartal period. Blood samples were taken from the cows involved in the experiment and values of parameters of the metabolic state were determined in 27 cows, of which 14 were primiparous and 13 multiparous. In the blood serum samples, we determined the concentration of glucose, total proteins, albumin, urea, and the activities of certain enzymes (alkaline phosphatase, ASAT and ALAT. It was established on the grounds of the obtained results that the delay in the establishment of reproductive activity in the postpartal period was in correlation with the blood concentrations of urea and albumin. .

  12. Visual Interaction Activated from Figures′ Background Exchange in Graphics Design%论平面设计中图底互换形式诱发的视觉互动

    Institute of Scientific and Technical Information of China (English)

    谢筱冬

    2012-01-01

    It analyzed the visual perception and its conversion of figures′ background exchange forms from Gestalt psychology′s perspective.It summarizes the two main forms of figures′ background exchange.Combined with some examples of graphic designs,it discussed the figures′ background relationships which could activate viewers′ visual interaction.It put forward the idea that in graphics design,artists should put the creativity in the first place,and the visual psychology rules should be the basis for artists while creating a unique and thought-provoking figure′s background exchange form.By this,it could activate viewers′ visual interaction and induce the viewers to interpret the art works actively,so as to achieve the best state of visual information conveying process.%从格式塔心理学的角度分析了图底互换形式的视知觉感知和转换,归纳了图底互换的2种主要构成形式,并结合平面设计中的一些实例,探讨了能较好诱发观者视觉互动的图底关系。提出在平面设计中,艺术家应以创意为先导、以视觉心理规律为依据,有目的、有意识地创造出独特又耐人寻味的图底互换形式,以此引发观者视觉上的互动,诱导观者积极主动地释读作品,从而达到视觉信息传达过程的最佳状态。

  13. Activity Profile and Between-Match Variation in Elite Male Field Hockey.

    Science.gov (United States)

    Sunderland, Caroline D; Edwards, Phillip L

    2017-03-01

    Sunderland, CD and Edwards, PL. Activity profile and between-match variation in elite male field hockey. J Strength Cond Res 31(3): 758-764, 2017-This study aimed to (a) provide a position-specific activity profile for elite male hockey players, (b) determine if the activity profile was altered by the introduction of the "self-pass" rule, and (c) provide information relating to match-to-match variability in elite male field hockey. The activity of 28 elite male field hockey players was analyzed over 2 seasons totaling 395 player-match analyses using Global Positioning Satellite technology. Total distance, high-speed running (>15.5 km·h), sprinting (>20 km·h), and mean speed were recorded. Players were categorized into 4 positions: fullback (FB), halfback (HB), midfield (M), and forward (F). Data were analyzed using a 2-way analysis of variance (season, position) and between-match coefficients of variation (CV). The time played differs with position (FB: 65.5 ± 5.3, HB: 49.5 ± 11.5, M: 45.9 ± 7.1, F: 39.5 ± 5.4 minutes; p < 0.0005) and thus affected the activity profile. Total distance covered was greater for fullbacks (FB: 8,001 ± 447, HB: 6,435 ± 1,399, M: 6,415 ± 908, F: 5,844 ± 762 m, p < 0.001), and mean speed and percentage time spent high-speed running and sprinting were greater for forwards than all other positions (HSR: FB: 6.8 ± 1.0, HB: 8.8 ± 1.3, M: 10.7 ± 1.2, F: 13.5 ± 1.8%, p < 0.001). The activity profile did not differ with the introduction of the self-pass. Match-to-match variability (CV) ranged from 5.0% to 22.0% for total and sprint distance, respectively. This is the first study to present an activity profile of elite men's field hockey and its associated variability and demonstrates that each position is unique, and therefore, training and recovery should be position specific.

  14. The Increasing Impact of Activity-Based Protein Profiling in Plant Science.

    Science.gov (United States)

    Morimoto, Kyoko; van der Hoorn, Renier A L

    2016-03-01

    The active proteome dictates plant physiology. Yet, active proteins are difficult to predict based on transcript or protein levels, because protein activities are regulated post-translationally in their microenvironments. Over the past 10 years, activity-based protein profiling (ABPP) is increasingly used in plant science. ABPP monitors the activities of hundreds of plant proteins using tagged chemical probes that react with the active site of proteins in a mechanism-dependent manner. Since labeling is covalent and irreversible, labeled proteins can be detected and identified on protein gels and by mass spectrometry using tagged fluorophores and/or biotin. Here, we discuss general concepts, approaches and practical considerations of ABPP, before we summarize the discoveries made using 40 validated probes representing 14 chemotypes that can monitor the active state of >4,500 plant proteins. These discoveries and new opportunities indicate that this emerging functional proteomic technology is a powerful discovery tool that will have an increasing impact on plant science.

  15. Configurable software for satellite graphics

    Energy Technology Data Exchange (ETDEWEB)

    Hartzman, P D

    1977-12-01

    An important goal in interactive computer graphics is to provide users with both quick system responses for basic graphics functions and enough computing power for complex calculations. One solution is to have a distributed graphics system in which a minicomputer and a powerful large computer share the work. The most versatile type of distributed system is an intelligent satellite system in which the minicomputer is programmable by the application user and can do most of the work while the large remote machine is used for difficult computations. At New York University, the hardware was configured from available equipment. The level of system intelligence resulted almost completely from software development. Unlike previous work with intelligent satellites, the resulting system had system control centered in the satellite. It also had the ability to reconfigure software during realtime operation. The design of the system was done at a very high level using set theoretic language. The specification clearly illustrated processor boundaries and interfaces. The high-level specification also produced a compact, machine-independent virtual graphics data structure for picture representation. The software was written in a systems implementation language; thus, only one set of programs was needed for both machines. A user can program both machines in a single language. Tests of the system with an application program indicate that is has very high potential. A major result of this work is the demonstration that a gigantic investment in new hardware is not necessary for computing facilities interested in graphics.

  16. Graphics editors in CPDev environment

    Directory of Open Access Journals (Sweden)

    Marcin Jamro

    2012-03-01

    Full Text Available According to IEC 61131-3 norm, controllers and distributed control systems can be programmed in textual and graphical languages. In many scenarios using a graphical language is preferred by the user, because diagrams can be more legible and easier to understand or modify also by people who do not have strong programming skills. What is more, they can be attached to the documentation to present a part of a system implementation. CPDev is an engineering environment that makes possible to program PLCs, PACs, softPLCs and distributed control systems with the usage of languages defined in IEC 61131-3 norm. In earlier versions, it supported only textual languages - ST and IL. Currently, graphics editors for FBD, LD and SFC languages are also available, so users can choose a suitable language depending on their skills and a specificity of a program that they have to prepare. The article presents implementation of the graphics editors, made by the author, which support creating program organization units in all graphical languages defined in IEC 61131-3 norm. They are equipped with a set of basic and complex functionalities to provide an easy and intuitive way of creating programs, function blocks and functions with visual programming. In the article the project structure and some important mechanisms are described. They include e.g. automatic connections finding (with A* algorithm, translation to ST code, conversion to and from XML format and an execution mode supporting multiple data sources and breakpoints.

  17. Antinomies of Semiotics in Graphic Design

    Science.gov (United States)

    Storkerson, Peter

    2010-01-01

    The following paper assesses the roles played by semiotics in graphic design and in graphic design education, which both reflects and shapes practice. It identifies a series of factors; graphic design education methods and culture; semiotic theories themselves and their application to graphic design; the two wings of Peircian semiotics and…

  18. Cartooning History: Canada's Stories in Graphic Novels

    Science.gov (United States)

    King, Alyson E.

    2012-01-01

    In recent years, historical events, issues, and characters have been portrayed in an increasing number of non-fiction graphic texts. Similar to comics and graphic novels, graphic texts are defined as fully developed, non-fiction narratives told through panels of sequential art. Such non-fiction graphic texts are being used to teach history in…

  19. Graphics-System Color-Code Interface

    Science.gov (United States)

    Tulppo, J. S.

    1982-01-01

    Circuit originally developed for a flight simulator interfaces a computer graphics system with color monitor. Subsystem is intended for particular display computer (AGT-130, ADAGE Graphics Terminal) and specific color monitor (beam penetration tube--Penetron). Store-and-transmit channel is one of five in graphics/color-monitor interface. Adding 5-bit color code to existing graphics programs requires minimal programing effort.

  20. Vocational Teaching Cube System of Engineering Graphics

    Institute of Scientific and Technical Information of China (English)

    YangDaofu; LiuShenli

    2003-01-01

    Based on long-time research on vocational teaching cube theory in graphics education and analyzing on the intellectual structure in the process of reading engineering drawing, the graphics intellectual three-dimensional model, which is made up of 100 cubes, is founded and tested in higher vocational graphics education. This system serves as a good guidance to the graphics teaching.

  1. Activity profiling of vacuolar processing enzymes reveals a role for VPE during oomycete infection.

    Science.gov (United States)

    Misas-Villamil, Johana C; Toenges, Gerrit; Kolodziejek, Izabella; Sadaghiani, Amir M; Kaschani, Farnusch; Colby, Thomas; Bogyo, Matthew; van der Hoorn, Renier A L

    2013-02-01

    Vacuolar processing enzymes (VPEs) are important cysteine proteases that are implicated in the maturation of seed storage proteins, and programmed cell death during plant-microbe interactions and development. Here, we introduce a specific, cell-permeable, activity-based probe for VPEs. This probe is highly specific for all four Arabidopsis VPEs, and labeling is activity-dependent, as illustrated by sensitivity for inhibitors, pH and reducing agents. We show that the probe can be used for in vivo imaging and displays multiple active isoforms of VPEs in various tissues and in both monocot and dicot plant species. Thus, VPE activity profiling is a robust, simple and powerful tool for plant research for a wide range of applications. Using VPE activity profiling, we discovered that VPE activity is increased during infection with the oomycete pathogen Hyaloperonospora arabidopsidis (Hpa). The enhanced VPE activity is host-derived and EDS1-independent. Sporulation of Hpa is reduced on vpe mutant plants, demonstrating a role for VPE during compatible interactions that is presumably independent of programmed cell death. Our data indicate that, as an obligate biotroph, Hpa takes advantage of increased VPE activity in the host, e.g. to mediate protein turnover and nutrient release.

  2. Comparative Analysis of Click Chemistry Mediated Activity-Based Protein Profiling in Cell Lysates

    Directory of Open Access Journals (Sweden)

    Yinliang Yang

    2013-10-01

    Full Text Available Activity-based protein profiling uses chemical probes that covalently attach to active enzyme targets. Probes with conventional tags have disadvantages, such as limited cell permeability or steric hindrance around the reactive group. A tandem labeling strategy with click chemistry is now widely used to study enzyme targets in situ and in vivo. Herein, the probes are reacted in live cells, whereas the ensuing detection by click chemistry takes place in cell lysates. We here make a comparison of the efficiency of the activity-based tandem labeling strategy by using Cu(I-catalyzed and strain-promoted click chemistry, different ligands and different lysis conditions.

  3. Profile of elderly people with high blood pressure in a family physical activity program

    Directory of Open Access Journals (Sweden)

    Luzia Wilma Santana da Silva

    2015-05-01

    Full Text Available A quantitative and descriptive study, aimed at analyzing the socioeconomic and behavioral profile of elderly people with hypertension interested in participating in a regular physical activity program with their families. The results showed people that mainly belong to the C class, which adopt physically active behavior to the routines of housework in their daily lives. The findings, allows to infer that for specific health promotion arises as necessary regular physical activity as a commitment to add to the home daily demands to health potential.  

  4. Relativistic Hydrodynamics on Graphic Cards

    CERN Document Server

    Gerhard, Jochen; Bleicher, Marcus

    2012-01-01

    We show how to accelerate relativistic hydrodynamics simulations using graphic cards (graphic processing units, GPUs). These improvements are of highest relevance e.g. to the field of high-energetic nucleus-nucleus collisions at RHIC and LHC where (ideal and dissipative) relativistic hydrodynamics is used to calculate the evolution of hot and dense QCD matter. The results reported here are based on the Sharp And Smooth Transport Algorithm (SHASTA), which is employed in many hydrodynamical models and hybrid simulation packages, e.g. the Ultrarelativistic Quantum Molecular Dynamics model (UrQMD). We have redesigned the SHASTA using the OpenCL computing framework to work on accelerators like graphic processing units (GPUs) as well as on multi-core processors. With the redesign of the algorithm the hydrodynamic calculations have been accelerated by a factor 160 allowing for event-by-event calculations and better statistics in hybrid calculations.

  5. Fractal geometry and computer graphics

    CERN Document Server

    Sakas, Georgios; Peitgen, Heinz-Otto; Englert, Gabriele

    1992-01-01

    Fractal geometry has become popular in the last 15 years, its applications can be found in technology, science, or even arts. Fractal methods and formalism are seen today as a general, abstract, but nevertheless practical instrument for the description of nature in a wide sense. But it was Computer Graphics which made possible the increasing popularity of fractals several years ago, and long after their mathematical formulation. The two disciplines are tightly linked. The book contains the scientificcontributions presented in an international workshop in the "Computer Graphics Center" in Darmstadt, Germany. The target of the workshop was to present the wide spectrum of interrelationships and interactions between Fractal Geometry and Computer Graphics. The topics vary from fundamentals and new theoretical results to various applications and systems development. All contributions are original, unpublished papers.The presentations have been discussed in two working groups; the discussion results, together with a...

  6. Anthropometric profile and habits of physical activity of a scho ol students Mapuches rural Temuco, Chile

    Directory of Open Access Journals (Sweden)

    Pablo Antonio Valdés-Badilla

    2015-04-01

    Full Text Available Introduction: The nutritional status of children can be a predictor of health in adulthood element, the Mapuche population has a non-Mapuche population that increased prevalence of obesity in Chile. The aim of this study was to determine the anthropometric profile and physical activity habits of Mapuche students of a particular charter school in the rural of Temuco city.Material and Methods: The design is not experimental, descriptive, transversal, with a quantitative approach. The sample included all students of the educational establishment Mapuches (n=23, in both sexes. He underwent anthropometric assessment ISAK and total time physical activity was estimated by the International Physical Activity Questionnaire, short version in Spanish (IPAQ-A. To correlate the variables a partial correlation was used.Results: The students average about 35.4% of fat mass, muscle mass 34.1%, 11.4% of residual mass, 12% of bone mass, 7% of residual mass and somatotype 4.9–5.1–1.8 that meso-endomorph classified as balanced. Regarding the total time physical activity, students reach 2225.9 minutes/week, your energy expenditure is 9592.1 (METs/min/week and stay seated equals 228.6 minutes per week, finally negative correlation was found between BMI and total time of physical activity.Conclusions: The students have a mostly standard anthropometric profile, but with a troubling obesity rate. His activity level is high, placing them as active subjects according to normative tables.

  7. Graphic Journeys: Graphic Novels' Representations of Immigrant Experiences

    Science.gov (United States)

    Boatright, Michael D.

    2010-01-01

    This article explores how immigrant experiences are represented in the narratives of three graphic novels published in the last decade: Tan's (2007) "The Arrival," Kiyama's (1931/1999) "The Four Immigrants Manga: A Japanese Experience in San Francisco, 1904-1924," and Yang's (2006) "American Born Chinese." Through a theoretical lens informed by…

  8. STORYBOARD DALAM PEMBUATAN MOTION GRAPHIC

    Directory of Open Access Journals (Sweden)

    Satrya Mahardhika

    2013-09-01

    screenplay, character, environment design and storyboards. The storyboard will be determined through camera angles, blocking, sets, and many supporting roles involved in a scene. Storyboard is also useful as a production reference in recording or taping each scene in sequence or as an efficient priority. The example used is an ad creation using motion graphic animation storyboard which has an important role as a blueprint for every scene and giving instructions to make the transition movement, layout, blocking, and defining camera movement that everything should be done periodically in animation production. Planning before making the animation or motion graphic will make the job more organized, presentable, and more efficient in the process.

  9. Botanical origin causes changes in nutritional profile and antioxidant activity of fermented products obtained from honey.

    Science.gov (United States)

    Dezmirean, Graţia I; Mărghitaş, Liviu A; Bobiş, Otilia; Dezmirean, Daniel S; Bonta, Victoriţa; Erler, Silvio

    2012-08-15

    Honey as rich source of enzymatic and nonenzymatic antioxidants serves as health-promoting nutrient in the human body. Here, we present the first time a comparative study of nutritional profiles (e.g., acidities, sugar, organic acid profile, total polyphenolic, flavonoid content) for different unifloral, multifloral honeys and their fermented products, in correlation with their antioxidant activity. Additionally, an optimized method for HPLC separation of organic acids from honey was established. The total phenolic content of honey samples varied widely among the honey types compared to fermented products. High amounts of total flavonoids were quantified in heather honey, followed by raspberry, multifloral, black locust, and linden honey. A positive correlation between the content of polyphenols, flavonoids, and antioxidant activity was observed in honey samples. After fermentation, the flavonoid content of dark honey fermented products decreased significantly. Black locust and linden honeys are more suitable for fermentation because the decrease in antioxidant substances is less pronounced.

  10. Ontogenetic changes in digestive enzyme activities and the amino acid profile of starry flounder Platichthys stellatus

    Science.gov (United States)

    Song, Zhidong; Wang, Jiying; Qiao, Hongjin; Li, Peiyu; Zhang, Limin; Xia, Bin

    2016-09-01

    Ontogenetic changes in digestive enzyme activities and the amino acid (AA) profile of starry flounder, Platichthys stellatus, were investigated and limiting amino acids were estimated compared with the essential AA profile between larvae and live food to clarify starry flounder larval nutritional requirements. Larvae were collected at the egg stage and 0, 2, 4, 7, 12, 17, 24 days after hatching (DAH) for analysis. Larvae grew from 1.91 mm at hatching to 12.13 mm at 24 DAH. Trypsin and chymotrypsin activities changed slightly by 4 DAH and then increased significantly 4 DAH. Pepsin activity increased sharply beginning 17 DAH. Lipase activity increased significantly 4 DAH and increased progressively with larval growth. Amylase activity was also detected in newly hatched larvae and increased 7 DAH followed by a gradual decrease. High free amino acid (FAA) content was detected in starry flounder eggs (110.72 mg/g dry weight). Total FAA content dropped to 43.29 mg/g in 4-DAH larvae and then decreased gradually to 13.74 mg/g in 24-DAH larvae. Most FAAs (except lysine and methionine) decreased >50% in 4-DAH larvae compared with those in eggs and then decreased to the lowest values in 24-DAH larvae. Changes in the protein amino acid (PAA) profile were much milder than those observed for FAAs. Most PAAs increased gradually during larval development, except lysine and phenylalanine. The percentages of free threonine, valine, isoleucine, and leucine decreased until the end of the trial, whereas the protein forms of these four AAs followed the opposite trend. A comparison of the essential AA composition of live food (rotifers, Artemia nauplii, and Artemia metanauplii) and larvae suggested that methionine was potentially the first limiting AA. These results may help develop starry flounder larviculture methods by solving the AA imbalance in live food. Moreover, the increased digestive enzyme activities indicate the possibility of introducing artificial compound feed.

  11. Profiling Kinase Activity during Hepatitis C Virus Replication Using a Wortmannin Probe.

    Science.gov (United States)

    Desrochers, Geneviève F; Sherratt, Allison R; Blais, David R; Nasheri, Neda; Ning, Zhibin; Figeys, Daniel; Goto, Natalie K; Pezacki, John Paul

    2015-09-11

    To complete its life cycle, the hepatitis C virus (HCV) induces changes to numerous aspects of its host cell. As kinases act as regulators of many pathways utilized by HCV, they are likely enzyme targets for virally induced inhibition or activation. Herein, we used activity-based protein profiling (ABPP), which allows for the identification of active enzymes in complex protein samples and the quantification of their activity, to identify kinases that displayed differential activity in HCV-expressing cells. We utilized an ABPP probe, wortmannin-yne, based on the kinase inhibitor wortmannin, which contains a pendant alkyne group for bioconjugation using bioorthogonal chemistry. We observed changes in the activity of kinases involved in the mitogen-activated protein kinase pathway, apoptosis pathways, and cell cycle control. These results establish changes to the active kinome, as reported by wortmannin-yne, in the proteome of human hepatoma cells actively replicating HCV. The observed changes include kinase activity that affect viral entry, replication, assembly, and secretion, implying that HCV is regulating the pathways that it uses for its life cycle through modulation of the active kinome.

  12. Recorded Music and Graphic Design.

    Science.gov (United States)

    Osterer, Irv

    1998-01-01

    Reviews the history of art as an element of music-recording packaging. Describes a project in which students design a jacket for either cassette or CD using a combination of computerized and traditional rendering techniques. Reports that students have been inspired to look into careers in graphic design. (DSK)

  13. GRAPHIC INTERFACES FOR ENGINEERING APPLICATIONS

    Directory of Open Access Journals (Sweden)

    Ion PANA,

    2012-05-01

    Full Text Available Using effective the method of calculating Fitness for Service requires the achievement of graphical interfaces. This paper presents an example of such interfaces, made with Visual Basic program and used in the evaluation of pipelines in a research contract [4

  14. Fluid simulation for computer graphics

    CERN Document Server

    Bridson, Robert

    2008-01-01

    Animating fluids like water, smoke, and fire using physics-based simulation is increasingly important in visual effects, in particular in movies, like The Day After Tomorrow, and in computer games. This book provides a practical introduction to fluid simulation for graphics. The focus is on animating fully three-dimensional incompressible flow, from understanding the math and the algorithms to the actual implementation.

  15. Real-time volume graphics

    CERN Document Server

    Engel, Klaus; Kniss, Joe; Rezk-Salama, Christof; Weiskopf, Daniel

    2006-01-01

    Based on course notes of SIGGRAPH course teaching techniques for real-time rendering of volumetric data and effects; covers both applications in scientific visualization and real-time rendering. Starts with the basics (texture-based ray casting) and then improves and expands the algorithms incrementally. Book includes source code, algorithms, diagrams, and rendered graphics.

  16. Graphic Novels in the Classroom

    Science.gov (United States)

    Martin, Adam

    2009-01-01

    Today many authors and artists adapt works of classic literature into a medium more "user friendly" to the increasingly visual student population. Stefan Petrucha and Kody Chamberlain's version of "Beowulf" is one example. The graphic novel captures the entire epic in arresting images and contrasts the darkness of the setting and characters with…

  17. Active Learning Strategies for Phenotypic Profiling of High-Content Screens.

    Science.gov (United States)

    Smith, Kevin; Horvath, Peter

    2014-06-01

    High-content screening is a powerful method to discover new drugs and carry out basic biological research. Increasingly, high-content screens have come to rely on supervised machine learning (SML) to perform automatic phenotypic classification as an essential step of the analysis. However, this comes at a cost, namely, the labeled examples required to train the predictive model. Classification performance increases with the number of labeled examples, and because labeling examples demands time from an expert, the training process represents a significant time investment. Active learning strategies attempt to overcome this bottleneck by presenting the most relevant examples to the annotator, thereby achieving high accuracy while minimizing the cost of obtaining labeled data. In this article, we investigate the impact of active learning on single-cell-based phenotype recognition, using data from three large-scale RNA interference high-content screens representing diverse phenotypic profiling problems. We consider several combinations of active learning strategies and popular SML methods. Our results show that active learning significantly reduces the time cost and can be used to reveal the same phenotypic targets identified using SML. We also identify combinations of active learning strategies and SML methods which perform better than others on the phenotypic profiling problems we studied.

  18. Yields, Phenolic Profiles and Antioxidant Activities of Ziziphus jujube Mill. in Response to Different Fertilization Treatments

    Directory of Open Access Journals (Sweden)

    Min Wang

    2013-09-01

    Full Text Available Increasing demand for more jujube (Ziziphus jujube Mill. production requires understanding the specific fertilization needs of jujube trees. This study was conducted to compare fruit yields, phenolic profiles and antioxidant activity of jujube in response to different fertilizers. Application of organic fertilizer appeared to enhance the phenolics and antioxidant activity accumulation of jujubes, compared to conventional fertilized jujubes. Amongst inorganic fertilizers, supplemental potassium as an individual nutrient improved the accumulation of phenolics in jujubes. Our results demonstrate that phenolics levels and antioxidant activity of jujube can be manipulated through fertilizer management and tracked by following proanthocyanidin concentrations. In a practical production context, the combination of organic fertilizers and inorganic fertilizers such as more supplemental individual potassium, and less supplemental individual nitrogen and phosphorus, might be the best management combination for achieving higher phenolic concentration, stronger antioxidant activity and a good harvest.

  19. Comparison of phytochemical profiles, antioxidant and cellular antioxidant activities of different varieties of blueberry (Vaccinium spp.).

    Science.gov (United States)

    Wang, Huailing; Guo, Xinbo; Hu, Xiaodan; Li, Tong; Fu, Xiong; Liu, Rui Hai

    2017-02-15

    Numerous reports have demonstrated that the consumption of fruits and vegetables is beneficial for the human health. Blueberries, in particular, are rich in phytochemicals including free and bound forming. Phytochemical profiles of 14 varieties of blueberry were compared in this study. 12 compounds were analyzed and had significant changes in blueberry fruits. Total antioxidant activities in different blueberry varieties varied about 2.6times by oxygen radical absorbance capacity (ORAC) assay, and 2times by peroxyl radical scavenging capacity (PSC) assay. The cellular antioxidant activities (CAA) in different varieties varied about 3.9times without phosphate buffer saline (PBS) wash, and 4.7times with PBS wash by CAA assay. Blueberry extracts had potent antiproliferative activities against HepG2 human liver cancer cells, indicating the potential protective benefits associated with their use as functional foods. The anti-proliferative activity was observed to be dose-dependent in blueberry extracts.

  20. Teaching graphics in technical communication classes

    Science.gov (United States)

    Spurgeon, K. C.

    1981-01-01

    Graphic aids convey and clarify information more efficiently and accurately than words alone therefore, most technical writing includes the use of graphics. Ways of accumulating and presenting graphics illustrations on a shoestring budget are suggested. These include collecting graphics from companies, annual reports and laminating them for workshop use or putting them on a flip chart for classroom presentation, creating overhead transparencies to demonstrate different levels of effectiveness of graphic aids, and bringing in grahic artists for question/answer periods or in class workshops. Also included are an extensive handout as an introduction to graphics, sample assignments, and a selected and annotated bibliography.

  1. DGLa:A Distributed Graphics Language

    Institute of Scientific and Technical Information of China (English)

    潘志庚; 胡冰峰; 等

    1994-01-01

    A distributed graphics programming language called DGLa is presented,which facilitates the development of distributed graphics application.Facilities for distributed programming and graphics support are included in it,It not only supports synchronous and asynchronous communication but also provides programmer with multiple control mechanism for process communication.The graphics support of DGLa is powerful,for both sequential graphics library and parallel graphics library are provided.The design consideration and implementation experience are discussed in detail in this paper.Application examples are also given.

  2. Reverse-engineering graphical innovation: an introduction to graphical regimes

    Directory of Open Access Journals (Sweden)

    Dominic Arsenault

    2013-03-01

    Full Text Available Technological innovation in the video games industry is a rich area of research that has barely been explored as of yet. Gamers are always clamoring for novelty and a remedy to the oft-decried “sequelitis” that “plagues” the industry, while game publishers and platform holders secretly plan a next-gen platform to capture the ever-shifting market. In this light, the importance of graphics cannot be understated, as it is usually taken for granted in game historiography that “[g]ame graphics were, and to a large extent still are, the main criteria by which advancing video game technology is benchmarked” (Wolf, 2003, p.53.

  3. Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland

    Science.gov (United States)

    Ruiz-Rodríguez, Magdalena; Valdivia, Eva; Martín-Vivaldi, Manuel; Martín-Platero, Antonio M.; Martínez-Bueno, Manuel; Méndez, María; Peralta-Sánchez, Juan M.; Soler, Juan J.

    2012-01-01

    Symbiotic microorganisms may be directly transferred from parents to offspring or acquired from a particular environment that animals may be able to select. If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one. Enterococci symbionts living in the hoopoe (Upupa epops) uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria). We explored variability in genetic profile (through RAPD-PCR analyses) and antimicrobial properties (by performing antagonistic tests against ten bacterial indicator strains) of the different isolates obtained from the uropygial glands of hoopoe females and nestlings. We found that the genetic profile of bacterial isolates was related to antimicrobial activity, as well as to individual host identity and the nest from which samples were obtained. This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection. PMID:22911858

  4. Effects of high hydrostatic pressure on genomic expression profiling of porcine parthenogenetic activated and cloned embryos

    DEFF Research Database (Denmark)

    Lin, Lin; Luo, Yonglun; Sørensen, Peter;

    2014-01-01

    mechanism underlying the effects of HHP treatment on embryonic development is poorly understood and so was investigated in the present study. Thus, in the present study, we undertook genome-wide gene expression analysis in HHP-treated and untreated oocytes, as well as in 4-cell and blastocyst stage embryos...... derived by PA or HMC. Hierarchical clustering depicted stage-specific genomic expression profiling. At the 4-cell and blastocyst stages, 103 and 163 transcripts were differentially expressed between the HMC and PA embryos, respectively (P ... (INHBB and ME3) were further validated by quantitative reverse transcription–polymerase chain reaction. We also observed that HHP treatment activated expression of the imprinting gene DLX5 in 4-cell PA embryos. In conclusion, our genomic expression profiling data suggest that HHP alters the RNA...

  5. FUNCTIONAL PROFILE OF ACTIVE OLDER ADULTS WITH LOW BACK PAIN, ACCORDING TO THE ICF

    Directory of Open Access Journals (Sweden)

    Andersom Ricardo Fréz

    Full Text Available ABSTRACT Introduction: The International Classification of Functioning, Disability and Health (ICF considers multiples aspects of functionality. It is believed that this tool can help to classify the functionality of older adults with low back pain (LBP . Objectives: To describe the functionality of active older adults with LBP according to the ICF. Methods: A transversal study was conducted using the brief ICF core set for low back pain, to establish functional profiles of 40 older adults. The ICF categories were considered valid when ≥20% of participants showed some disability. Results: Thirty-two of the 35 categories of the brief ICF core set could be considered representative of the sample. Conclusion: The brief ICF core set for LBP results demonstrated that this classification system is representative for describing the functional profile of the sample.

  6. Antimicrobial activity and genetic profile of Enteroccoci isolated from hoopoes uropygial gland.

    Science.gov (United States)

    Ruiz-Rodríguez, Magdalena; Valdivia, Eva; Martín-Vivaldi, Manuel; Martín-Platero, Antonio M; Martínez-Bueno, Manuel; Méndez, María; Peralta-Sánchez, Juan M; Soler, Juan J

    2012-01-01

    Symbiotic microorganisms may be directly transferred from parents to offspring or acquired from a particular environment that animals may be able to select. If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one. Enterococci symbionts living in the hoopoe (Upupa epops) uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria). We explored variability in genetic profile (through RAPD-PCR analyses) and antimicrobial properties (by performing antagonistic tests against ten bacterial indicator strains) of the different isolates obtained from the uropygial glands of hoopoe females and nestlings. We found that the genetic profile of bacterial isolates was related to antimicrobial activity, as well as to individual host identity and the nest from which samples were obtained. This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection.

  7. Antimicrobial activity and genetic profile of Enteroccoci isolated from hoopoes uropygial gland.

    Directory of Open Access Journals (Sweden)

    Magdalena Ruiz-Rodríguez

    Full Text Available Symbiotic microorganisms may be directly transferred from parents to offspring or acquired from a particular environment that animals may be able to select. If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one. Enterococci symbionts living in the hoopoe (Upupa epops uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria. We explored variability in genetic profile (through RAPD-PCR analyses and antimicrobial properties (by performing antagonistic tests against ten bacterial indicator strains of the different isolates obtained from the uropygial glands of hoopoe females and nestlings. We found that the genetic profile of bacterial isolates was related to antimicrobial activity, as well as to individual host identity and the nest from which samples were obtained. This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection.

  8. Quercetin changes purinergic enzyme activities and oxidative profile in platelets of rats with hypothyroidism.

    Science.gov (United States)

    Baldissarelli, Jucimara; Santi, Adriana; Schmatz, Roberta; Zanini, Daniela; Cardoso, Andréia M; Abadalla, Fátima H; Thomé, Gustavo R; Murussi, Camila; Polachini, Carla R N; Delenogare, Diéssica P; Loro, Vania L; Morsch, Vera M; Schetinger, Maria R C

    2016-12-01

    Diseases related to thyroid hormones have been extensively studied because affect a large number of individuals, and these hormones participate in the regulation of the whole organism homeostasis. However, little is known about the involvement of purinergic signaling related to oxidative stress in hypothyroidism and possible therapeutic adjuncts for treatment of this disorder. Thus, the present study investigates the effects of quercetin on NTPDase, 5'-nucleotidase and adenosine deaminase activities, platelet aggregation and oxidative profile in platelets of rats with methimazole (MMI)-induced hypothyroidism. Methimazole at a concentration of 20mg/100mL was administered for 90days. From the second month the animals received quercetin 10 or 25mg/kg for 60days. Results showed that: Ecto-5'-nucleotidase activity decreased in methimazole/water group and the treatment with quercetin 25mg/kg decreased NTPDase, 5'-nucleotidase and adenosine deaminase activities. Moreover, platelet aggregation increased in methimazole/water group. Lipid peroxidation increased while superoxide dismutase and catalase activities decreased, but, interestingly, the treatment with quercetin reversed these changes. These results demonstrated that quercetin modulates adenine nucleotide hydrolysis decreasing the ADP formation and adenosine deamination. At the same time quercetin improves the oxidative profile, as well as reduces platelet aggregation, which together with the modulation in the nucleotides levels can contribute to the prevention of platelet disorders.

  9. Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Ines eLassowskat

    2014-10-01

    Full Text Available Mitogen-activated protein kinases (MAPKs target a variety of protein substrates to regulate cellular signaling processes in eukaryotes. In plants, the number of identified MAPK substrates that control plant defense responses is still limited. Here, we generated transgenic Arabidopsis thaliana plants with an inducible system to simulate in vivo activation of two stress-activated MAPKs, MPK3 and MPK6. Metabolome analysis revealed that this artificial MPK3/6 activation (without any exposure to pathogens or other stresses is sufficient to drive the production of major defense-related metabolites, including various camalexin, indole glucosinolate and agmatine derivatives. An accompanying (phosphoproteome analysis led to detection of hundreds of potential phosphoproteins downstream of MPK3/6 activation. Besides known MAPK substrates, many candidates on this list possess typical MAPK-targeted phosphosites and in many cases, the corresponding phosphopeptides were detected by mass spectrometry. Notably, several of these putative phosphoproteins have been reported to be associated with the biosynthesis of antimicrobial defense substances (e.g. WRKY transcription factors and proteins encoded by the genes from the PEN pathway required for penetration resistance to filamentous pathogens. Thus, this work provides an inventory of candidate phosphoproteins, including putative direct MAPK substrates, for future analysis of MAPK-mediated defense control. (Proteomics data are available with the identifier PXD001252 via ProteomeXchange, http://proteomecentral.proteomexchange.org.

  10. Intuitive Music and Graphic Notation

    DEFF Research Database (Denmark)

    Bergstrøm-Nielsen, Carl

    Describes subjects existing at Aalborg University since the middle eighties. "Intuitive Music" trains free improvisation through exercises including group-dynamic exercises, awareness exercises and parameter exercises. Students also create open compositions. "Graphic notation"concerns aural scores....... Students' works are quoted. The writer discusses the theoretical context and advocates for giving more attention to music as the medium in which music therapy takes place, referring to language theory and Jakobson. NB: the description of the two subjects are, at the present moment (2011) no longer up...... to date. Intuitive music stresses less making compositions and more using the main instrument intuitively. Graphic notation has been integrated into a larger subject (also taught by the present author) which also comprises other methods of description and interpretation of music....

  11. Approximating Graphic TSP by Matchings

    CERN Document Server

    Mömke, Tobias

    2011-01-01

    We present a framework for approximating the metric TSP based on a novel use of matchings. Traditionally, matchings have been used to add edges in order to make a given graph Eulerian, whereas our approach also allows for the removal of certain edges leading to a decreased cost. For the TSP on graphic metrics (graph-TSP), the approach yields a 1.461-approximation algorithm with respect to the Held-Karp lower bound. For graph-TSP restricted to a class of graphs that contains degree three bounded and claw-free graphs, we show that the integrality gap of the Held-Karp relaxation matches the conjectured ratio 4/3. The framework allows for generalizations in a natural way and also leads to a 1.586-approximation algorithm for the traveling salesman path problem on graphic metrics where the start and end vertices are prespecified.

  12. Image reproduction with interactive graphics

    Science.gov (United States)

    Buckner, J. D.; Council, H. W.; Edwards, T. R.

    1974-01-01

    Software application or development in optical image digital data processing requires a fast, good quality, yet inexpensive hard copy of processed images. To achieve this, a Cambo camera with an f 2.8/150-mm Xenotar lens in a Copal shutter having a Graflok back for 4 x 5 Polaroid type 57 pack-film has been interfaced to an existing Adage, AGT-30/Electro-Mechanical Research, EMR 6050 graphic computer system. Time-lapse photography in conjunction with a log to linear voltage transformation has resulted in an interactive system capable of producing a hard copy in 54 sec. The interactive aspect of the system lies in a Tektronix 4002 graphic computer terminal and its associated hard copy unit.

  13. Language teaching and graphic colonial.

    Directory of Open Access Journals (Sweden)

    Sandra Yaneth Chaparro Cardozo

    2015-03-01

    Full Text Available In the present study a reflection of the colonial baroque ornamentation from education and graphic blocks; analyzing aspects such as creating maps of the Basilica Church Cathedral Santiago de Tunja. This research tests the interpretation of the drawing and construction of ornamental figures of the native nature of the region in the colonies of the city of Tunja, Boyacá churches. This study of the visual reconstruction of the routes that make a group of children on the reinterpretation of the design in the construction of maps of colonial baroque in the creation and graphic composition. Given the importance of aesthetics in the visual language manuals maples ornaments of the cathedral with a look from the pedagogy and education in studies of iconography Erwin Panofsky in understanding the phenomenon of space. 

  14. GnuForPlot Graphics

    Energy Technology Data Exchange (ETDEWEB)

    2015-11-04

    Gnuforplot Graphics is a Fortran90 program designed to generate two and three dimensional plots of data on a personal computer. The program uses calls to the open source code Gnuplot to generate the plots. Two Fortran90 programs have been written to use the Gnuplot graphics capabilities. The first program, named Plotsetup.f90 reads data from output files created by either the Stadium or LeachXS/Orchestra modeling codes and saves the data in arrays for plotting. This program then calls Gnuforplot which takes the data array along with user specified parameters to set plot specifications and issues Gnuplot commands that generate the screen plots. The user can view the plots and optionally save copies in jpeg format.

  15. Graphical Multiprocessing Analysis Tool (GMAT)

    Energy Technology Data Exchange (ETDEWEB)

    Seager, M.K.; Campbell, S.; Sikora, S.; Strout, R.; Zosel, M.

    1988-03-01

    The design and debugging of parallel programs is a difficult task due to the complex synchronization and data scoping issues involed. to aid the programmer in paralle code dvelopment we have developed two methodologies for the graphical display of execution of parallel codes. The Graphical Multiprocessing Analysis Tools (GMAT) consist of stategraph, which represents an inheritance tree of task states, and timeline, which represens task as flowing sequence of events. Information about the code can be displayed as the application runs (dynamic mode) or played back with time under user control (static mode). This document discusses the design and user interface issues involved in developing the parallel application display GMAT family. Also, we present an introductory user's guide for both tools. 4 figs.

  16. Polyphenolic Profile and Antioxidant Activities of Oolong Tea Infusion under Various Steeping Conditions

    Directory of Open Access Journals (Sweden)

    Feng Chen

    2007-11-01

    Full Text Available The phenolic profile and antioxidant activities of oolong tea extract wereinvestigated after tea was steeped in 90 or 100 oC water for 3 or 10 min. The extractionyield increased with increasing temperature and extended steeping time. However, highertemperature and longer time (100 oC water for 10 min led to loss of phenolics. Theaqueous extract of oolong tea (AEOT at 100 oC for 3 min exhibited the strongestantioxidant activity. The major polyphenolic components of the AEOT were identified as(--epigallocatechin (EGC, (--epigallocatechin gallate (EGCG and (--epicatechin-3-gallate (ECG. The two major catechins (EGC and EGCG in the tea infusion contributedsignificantly to the investigated antioxidant activities [i.e., the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH radical scavenging and superoxide radical scavengingactivities] with high correlation values in r = 0.9486 and 0.9327 for the EGC and r =0.9592 and 0.8718 for the EGCG, respectively.

  17. An Active High Impedance Surface for Low Profile Tunable and Steerable Antennas

    CERN Document Server

    Costa, Filippo; Talarico, Salvatore; Valeri, Fabio M

    2009-01-01

    In this letter, an approach for designing a tunable and steerable antenna is presented. The antenna model is based on a wideband bow-tie radiating element mounted above an active Artificial Magnetic Conductor (AMC). The AMC geometry consists of a Frequency Selective Surface (FSS) printed on a thin grounded dielectric slab in which some chip-set varactor diodes are placed between the metallic elements and the backing plane through vias. The resulting antenna can be tuned over the S-Band by simply changing all varactor capacitances through an appropriate biasing voltage. Moreover, this structure can operate a beam scanning over each working frequency by applying an appropriate biasing voltage to the active elements of the AMC surface in accordance to leaky radiation principles. The low profile active antenna is characterized by an overall thickness of 5.32 mm, which corresponds to approximately lambda/24 at the centre of the operating band.

  18. Graphical Models for Bandit Problems

    CERN Document Server

    Amin, Kareem; Syed, Umar

    2012-01-01

    We introduce a rich class of graphical models for multi-armed bandit problems that permit both the state or context space and the action space to be very large, yet succinctly specify the payoffs for any context-action pair. Our main result is an algorithm for such models whose regret is bounded by the number of parameters and whose running time depends only on the treewidth of the graph substructure induced by the action space.

  19. Graphical Language for Data Processing

    Science.gov (United States)

    Alphonso, Keith

    2011-01-01

    A graphical language for processing data allows processing elements to be connected with virtual wires that represent data flows between processing modules. The processing of complex data, such as lidar data, requires many different algorithms to be applied. The purpose of this innovation is to automate the processing of complex data, such as LIDAR, without the need for complex scripting and programming languages. The system consists of a set of user-interface components that allow the user to drag and drop various algorithmic and processing components onto a process graph. By working graphically, the user can completely visualize the process flow and create complex diagrams. This innovation supports the nesting of graphs, such that a graph can be included in another graph as a single step for processing. In addition to the user interface components, the system includes a set of .NET classes that represent the graph internally. These classes provide the internal system representation of the graphical user interface. The system includes a graph execution component that reads the internal representation of the graph (as described above) and executes that graph. The execution of the graph follows the interpreted model of execution in that each node is traversed and executed from the original internal representation. In addition, there are components that allow external code elements, such as algorithms, to be easily integrated into the system, thus making the system infinitely expandable.

  20. Graphical Classification of Entangled Qutrits

    Directory of Open Access Journals (Sweden)

    Kentaro Honda

    2012-10-01

    Full Text Available A multipartite quantum state is entangled if it is not separable. Quantum entanglement plays a fundamental role in many applications of quantum information theory, such as quantum teleportation. Stochastic local quantum operations and classical communication (SLOCC cannot essentially change quantum entanglement without destroying it. Therefore, entanglement can be classified by dividing quantum states into equivalence classes, where two states are equivalent if each can be converted into the other by SLOCC. Properties of this classification, especially in the case of non two-dimensional quantum systems, have not been well studied. Graphical representation is sometimes used to clarify the nature and structural features of entangled states. SLOCC equivalence of quantum bits (qubits has been described graphically via a connection between tripartite entangled qubit states and commutative Frobenius algebras (CFAs in monoidal categories. In this paper, we extend this method to qutrits, i.e., systems that have three basis states. We examine the correspondence between CFAs and tripartite entangled qutrits. Using the symmetry property, which is required by the definition of a CFA, we find that there are only three equivalence classes that correspond to CFAs. We represent qutrits graphically, using the connection to CFAs. We derive equations that characterize the three equivalence classes. Moreover, we show that any qutrit can be represented as a composite of three graphs that correspond to the three classes.

  1. Community Level Physiological Profiles (CLPP, Characterization and Microbial Activity of Soil Amended with Dairy Sewage Sludge

    Directory of Open Access Journals (Sweden)

    Jerzy Lipiec

    2012-03-01

    Full Text Available The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland. Two doses of both dairy sewage sludge (20 Mg·ha−1 and 26 Mg·ha−1 and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential was assessed using such indices as Average Well Color Development (AWCD, Richness (R and Shannon–Weaver index (H. These indices were calculated, following the community level physiological profiling (CLPP using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA and principal component analysis (PCA were used to depict the differences of the soil bacterial functional diversity between the treatments.

  2. Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity.

    Directory of Open Access Journals (Sweden)

    Raúl Castanera

    Full Text Available BACKGROUND: The basidiomycete Pleurotus ostreatus is an efficient producer of laccases, a group of enzymes appreciated for their use in multiple industrial processes. The aim of this study was to reveal the molecular basis of the superiority of laccase production by dikaryotic strains compared to their parental monokaryons. METHODOLOGY/PRINCIPAL FINDINGS: We bred and studied a set of dikaryotic strains starting from a meiotic population of monokaryons. We then completely characterised the laccase allelic composition, the laccase gene expression and activity profiles in the dikaryotic strain N001, in two of its meiotic full-sib monokaryons and in the dikaryon formed from their mating. CONCLUSIONS/SIGNIFICANCE: Our results suggested that the dikaryotic superiority observed in laccase activity was due to non-additive transcriptional increases in lacc6 and lacc10 genes. Furthermore, the expression of these genes was divergent in glucose- vs. lignocellulose-supplemented media and was highly correlated to the detected extracellular laccase activity. Moreover, the expression profile of lacc2 in the dikaryotic strains was affected by its allelic composition, indicating a putative single locus heterozygous advantage.

  3. Towards art content in original graphic arts

    OpenAIRE

    2011-01-01

    Contemporary Graphic art defines numerous graphic art techniques; from classical original graphic art techniques to the new media techniques. The new media reproduction and communication capacity influences contemporary art works in such way that more attention is on non-artistic content, while artistic content with its visual efficiency becomes less important. Reproductibility is one of the non-artistic contents which plays a key role in original graphic arts, where it is a means of making o...

  4. Data structures, computer graphics, and pattern recognition

    CERN Document Server

    Klinger, A; Kunii, T L

    1977-01-01

    Data Structures, Computer Graphics, and Pattern Recognition focuses on the computer graphics and pattern recognition applications of data structures methodology.This book presents design related principles and research aspects of the computer graphics, system design, data management, and pattern recognition tasks. The topics include the data structure design, concise structuring of geometric data for computer aided design, and data structures for pattern recognition algorithms. The survey of data structures for computer graphics systems, application of relational data structures in computer gr

  5. Graphic filter library implemented in CUDA language

    OpenAIRE

    Peroutková, Hedvika

    2009-01-01

    This thesis deals with the problem of reducing computation time of raster image processing by parallel computing on graphics processing unit. Raster image processing thereby refers to the application of graphic filters, which can be applied in sequence with different settings. This thesis evaluates the suitability of using parallelization on graphic card for raster image adjustments based on multicriterial choice. Filters are implemented for graphics processing unit in CUDA language. Opacity ...

  6. Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses.

    Directory of Open Access Journals (Sweden)

    Ari J S Ferreira

    Full Text Available Metagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world's oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light.

  7. Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses.

    KAUST Repository

    Ferreira, Ari J S

    2014-06-12

    Metagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world\\'s oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs) of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light.

  8. A Method for Combined Passive-Active Microwave Retrievals of Cloud and Precipitation Profiles.

    Science.gov (United States)

    Olson, William S.; Kummerow, Christian D.; Heymsfield, Gerald M.; Giglio, Louis

    1996-10-01

    Three-dimensional tropical squall-line simulations from the Goddard cumulus ensemble (GCE) model are used as input to radiative computations of upwelling microwave brightness temperatures and radar reflectivities at selected microwave sensor frequencies. These cloud/radiative calculations form the basis of a physical cloud/precipitation profile retrieval method that yields estimates of the expected values of the hydrometeor water contents. Application of the retrieval method to simulated nadir-view observations of the aircraft-borne Advanced Microwave Precipitation Radiometer (AMPR) and NASA ER-2 Doppler radar (EDOP) produce random errors of 23%, 19%, and 53% in instantaneous estimates of integrated precipitating liquid, integrated precipitating ice, and surface rain rate, respectively.On 5 October 1993, during the Convection and Atmospheric Moisture Experiment (CAMEX), the AMPR and EDOP were used to observe convective systems in the vicinity of the Florida peninsula. Although the AMPR data alone could be used to retrieve cloud and precipitation vertical profiles over the ocean, retrievals of high-resolution vertical precipitation structure and profile information over land required the combination of AMPR and EDOP observations.No validation data are available for this study; however, the retrieved precipitation distributions from the convective systems are compatible with limited radar climatologies of such systems, as well as being radiometrically consistent with both the AMPR and EDOP observations. In the future, the retrieval method will be adapted to the passive and active microwave measurements from the Tropical Rainfall Measuring Mission (TRMM) satellite sensors.

  9. Proteome profile and biological activity of caprine, bovine and human milk fat globules.

    Science.gov (United States)

    Spertino, Stefano; Cipriani, Valentina; De Angelis, Chiara; Giuffrida, Maria Gabriella; Marsano, Francesco; Cavaletto, Maria

    2012-04-01

    Upon combining bidimensional electrophoresis with monodimensional separation, a more comprehensive analysis of the milk fat globule membrane has been obtained. The proteomic profile of caprine milk fat globules revealed the presence of butyrophilin, lactadherin and perilipin as the major proteins, they were also associated to bovine and human milk fat globule membranes. Xanthine dehydrogenase/oxidase has been detected only in monodimensional gels. Biological activity of milk fat globules has been evaluated in Caco2-cells, as a representative model of the intestinal barrier. The increase of cell viability was indicative of a potential nutraceutical role for the whole milk fat globule, suggesting a possible employment in milk formula preparation.

  10. Cardiovascular risk profile: Cross-sectional analysis of motivational determinants, physical fitness and physical activity

    Directory of Open Access Journals (Sweden)

    Kiers Henri

    2010-10-01

    Full Text Available Abstract Background Cardiovascular risk factors are associated with physical fitness and, to a lesser extent, physical activity. Lifestyle interventions directed at enhancing physical fitness in order to decrease the risk of cardiovascular diseases should be extended. To enable the development of effective lifestyle interventions for people with cardiovascular risk factors, we investigated motivational, social-cognitive determinants derived from the Theory of Planned Behavior (TPB and other relevant social psychological theories, next to physical activity and physical fitness. Methods In the cross-sectional Utrecht Police Lifestyle Intervention Fitness and Training (UP-LIFT study, 1298 employees (aged 18 to 62 were asked to complete online questionnaires regarding social-cognitive variables and physical activity. Cardiovascular risk factors and physical fitness (peak VO2 were measured. Results For people with one or more cardiovascular risk factors (78.7% of the total population, social-cognitive variables accounted for 39% (p In addition to the prediction of intention to engage in physical activity and physical active behavior, we explored the impact of the intensity of physical activity. The intentsity of physical activity was only significantly related to physical active behavior (beta = .253, p 2 = .06, p 2 = .23, p For people with one or more cardiovascular risk factors, 39.9% had positive intentions to engage in physical activity and were also physically active, and 10.5% had a low intentions but were physically active. 37.7% had low intentions and were physically inactive, and about 11.9% had high intentions but were physically inactive. Conclusions This study contributes to our ability to optimize cardiovascular risk profiles by demonstrating an important association between physical fitness and social-cognitive variables. Physical fitness can be predicted by physical active behavior as well as by self-efficacy and the intensity of

  11. Lean mainstream schemes for Stata 8 graphics

    DEFF Research Database (Denmark)

    Juul, Svend

    2003-01-01

    The new Stata 8 graphics are powerful and flexible. Now, a few months after the first release, the graphics still have some shortcomings—both in design and in the manual documenting the program—but progress is being made. The graph layout used throughout the Graphics Reference Manual has led some...

  12. Grid OCL : A Graphical Object Connecting Language

    Science.gov (United States)

    Taylor, I. J.; Schutz, B. F.

    In this paper, we present an overview of the Grid OCL graphical object connecting language. Grid OCL is an extension of Grid, introduced last year, that allows users to interactively build complex data processing systems by selecting a set of desired tools and connecting them together graphically. Algorithms written in this way can now also be run outside the graphical environment.

  13. Graphic Novels in Your School Library

    Science.gov (United States)

    Karp, Jesse

    2011-01-01

    Many educators now agree that graphic novels inform as well as entertain, and to dismiss the educational potential of the graphic novel is to throw away a golden opportunity to reach out to young readers. This dynamic book takes a look at the term "graphic novel," how the format has become entwined in our culture, and the ways in which graphic…

  14. Minard's Graphic of Napoleon in Russia.

    Science.gov (United States)

    Hardy, Charles

    1992-01-01

    Describes the use of Charles Minard's graphic of Napoleon's 1812 Russian Campaign as an instructional tool in history classes. Maintains that the graphic, created in 1861, can be analyzed by students to determine six historical and geographical factors involved in Napoleon's defeat. Includes a copy of Minard's graphic. (CFR)

  15. Narrative Problems of Graphic Design History.

    Science.gov (United States)

    Margolin, Victor

    1994-01-01

    Discusses three major accounts (by Philip Meggs, Enric Satue and Richard Hollis) of graphic design history. Notes that these texts address the history of graphic design, but each raises questions about what material to include, as well as how graphic design is both related to and distinct from other visual practices such as typography, art…

  16. A handbook of statistical graphics using SAS ODS

    CERN Document Server

    Der, Geoff

    2014-01-01

    An Introduction to Graphics: Good Graphics, Bad Graphics, Catastrophic Graphics and Statistical GraphicsThe Challenger DisasterGraphical DisplaysA Little History and Some Early Graphical DisplaysGraphical DeceptionAn Introduction to ODS GraphicsGenerating ODS GraphsODS DestinationsStatistical Graphics ProceduresODS Graphs from Statistical ProceduresControlling ODS GraphicsControlling Labelling in GraphsODS Graphics EditorGraphs for Displaying the Characteristics of Univariate Data: Horse Racing, Mortality Rates, Forearm Lengths, Survival Times and Geyser EruptionsIntroductionPie Chart, Bar Cha

  17. Effect of seafood mediated PCB exposure on desaturase activity and PUFA profile in Faroese septuagenarians.

    Science.gov (United States)

    Tøttenborg, Sandra Søgaard; Choi, Anna L; Bjerve, Kristian S; Weihe, Pal; Grandjean, Philippe

    2015-07-01

    Polychlorinated biphenyl (PCB) exposure may affect serum concentrations of polyunsaturated fatty acids (PUFAs) by inhibiting desaturases ∆5 and ∆6 that drive their synthesis from precursor fatty acids. Such changes in the composition of fatty acids may affect cardiovascular disease risk, which is thought to increase at elevated PCB exposures. This population-based cross-sectional study examined 712 Faroese men and women aged 70-74 years. The serum phospholipid fraction of fasting blood samples was used to determine the PUFA profile, including linoleic acid, dihomo-γ-linolenic acid, arachidonic acid, eicosatrienoic acid, and other relevant fatty acids. Ratios between precursor and metabolite fatty acids were used as proxies for ∆5 and ∆6 desaturase activity. Tertiles of serum-PCB concentrations were used in multiple regression analyses to determine the association between the exposure and desaturase activity. In multiple regression models, PCB exposure was inversely related to the estimated Δ6 desaturase activity resulting in accumulation of precursor fatty acids and decrease in the corresponding product PUFAs. A positive association between PCB and Δ5 desaturation was also found. A relative increase in EA was also observed, though only in the third tertile of PCB exposure. Non-linear relationships between the exposure and the desaturase activity were not found. Consuming fish and seafood may not be translated into beneficial fatty acid profiles if the diet simultaneously causes exposure to PCBs. Although the desaturase estimates were likely influenced by dietary intakes of product PUFAs, the association between PCB exposure and ∆6 desaturase activity is plausible and may affect cardiovascular disease risk.

  18. Modification of Pulsed Electric Field Conditions Results in Distinct Activation Profiles of Platelet-Rich Plasma

    Science.gov (United States)

    Frelinger, Andrew L.; Gerrits, Anja J.; Garner, Allen L.; Torres, Andrew S.; Caiafa, Antonio; Morton, Christine A.; Berny-Lang, Michelle A.; Carmichael, Sabrina L.; Neculaes, V. Bogdan; Michelson, Alan D.

    2016-01-01

    Background Activated autologous platelet-rich plasma (PRP) used in therapeutic wound healing applications is poorly characterized and standardized. Using pulsed electric fields (PEF) to activate platelets may reduce variability and eliminate complications associated with the use of bovine thrombin. We previously reported that exposing PRP to sub-microsecond duration, high electric field (SMHEF) pulses generates a greater number of platelet-derived microparticles, increased expression of prothrombotic platelet surfaces, and differential release of growth factors compared to thrombin. Moreover, the platelet releasate produced by SMHEF pulses induced greater cell proliferation than plasma. Aims To determine whether sub-microsecond duration, low electric field (SMLEF) bipolar pulses results in differential activation of PRP compared to SMHEF, with respect to profiles of activation markers, growth factor release, and cell proliferation capacity. Methods PRP activation by SMLEF bipolar pulses was compared to SMHEF pulses and bovine thrombin. PRP was prepared using the Harvest SmartPreP2 System from acid citrate dextrose anticoagulated healthy donor blood. PEF activation by either SMHEF or SMLEF pulses was performed using a standard electroporation cuvette preloaded with CaCl2 and a prototype instrument designed to take into account the electrical properties of PRP. Flow cytometry was used to assess platelet surface P-selectin expression, and annexin V binding. Platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), endothelial growth factor (EGF) and platelet factor 4 (PF4), and were measured by ELISA. The ability of supernatants to stimulate proliferation of human epithelial cells in culture was also evaluated. Controls included vehicle-treated, unactivated PRP and PRP with 10 mM CaCl2 activated with 1 U/mL bovine thrombin. Results PRP activated with SMLEF bipolar pulses or thrombin had similar light scatter profiles, consistent with the

  19. Volatile Profiling of Aromatic Traditional Medicinal Plant, Polygonum minus in Different Tissues and Its Biological Activities

    Directory of Open Access Journals (Sweden)

    Rafidah Ahmad

    2014-11-01

    Full Text Available The aim of this research was to identify the volatile metabolites produced in different organs (leaves, stem and roots of Polygonum minus, an important essential oil producing crop in Malaysia. Two methods of extraction have been applied: Solid Phase Microextraction (SPME and hydrodistillation coupled with Gas Chromatography-Mass Spectrometry (GC-MS. Approximately, 77 metabolites have been identified and aliphatic compounds contribute significantly towards the aroma and flavour of this plant. Two main aliphatic compounds: decanal and dodecanal were found to be the major contributor. Terpenoid metabolites were identified abundantly in leaves but not in the stem and root of this plant. Further studies on antioxidant, total phenolic content, anticholinesterase and antimicrobial activities were determined in the essential oil and five different extracts. The plant showed the highest DPPH radical scavenging activity in polar (ethanol extract for all the tissues tested. For anti-acetylcholinesterase activity, leaf in aqueous extract and methanol extract showed the best acetylcholinesterase inhibitory activities. However, in microbial activity, the non-polar extracts (n-hexane showed high antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA compared to polar extracts. This study could provide the first step in the phytochemical profiles of volatile compounds and explore the additional value of pharmacology properties of this essential oil producing crop Polygonum minus.

  20. Effect of Tomato Industrial Processing on Phenolic Profile and Antiplatelet Activity

    Directory of Open Access Journals (Sweden)

    Iván Palomo

    2013-09-01

    Full Text Available Background: Regular consumption of fruits and vegetables (e.g., tomatoes has been shown to be beneficial in terms of reducing the incidence of cardiovascular diseases. The industrial processing of tomatoes into tomato-based products includes several thermal treatments. Very little is known on the effect of tomato industrial processing on antiaggregatory activity and phenolic profile. Methods: It was assessed the effect of tomato and by-products extracts on platelet aggregation induced by ADP, collagen, TRAP-6 and arachidonic acid. These in vitro antithrombotic properties were further supported in an in vivo model of thrombosis. A set of antiplatelet compounds has been selected for HPLC analysis in the different extracts. Results: Some natural compounds such as chlorogenic, caffeic, ferulic and p-coumaric acids were identified by HPLC in tomatoes and its products may inhibit platelet activation. Red tomatoes, tomato products (sauce, ketchup and juice and by-products extracts inhibited platelet aggregation induced adenosine 5'-diphosphate, collagen, thrombin receptor activator peptide-6 and arachidonic acid, but to a different extent. Also, pomace extract presents antithrombotic activity. Conclusions: Processed tomatoes may have a higher content of health-benefiting compounds than fresh ones. Pomace even presents the best antiplatelet activity. Finally, tomato products may be used as a functional ingredient adding antiplatelet activities to processed foods.

  1. MicroRNA expression profiling identifies activated B cell status in chronic lymphocytic leukemia cells.

    Directory of Open Access Journals (Sweden)

    Shuqiang Li

    Full Text Available Chronic lymphocytic leukemia (CLL is thought to be a disease of resting lymphocytes. However, recent data suggest that CLL cells may more closely resemble activated B cells. Using microRNA (miRNA expression profiling of highly-enriched CLL cells from 38 patients and 9 untransformed B cells from normal donors before acute CpG activation and 5 matched B cells after acute CpG activation, we demonstrate an activated B cell status for CLL. Gene set enrichment analysis (GSEA identified statistically-significant similarities in miRNA expression between activated B cells and CLL cells including upregulation of miR-34a, miR-155, and miR-342-3p and downregulation of miR-103, miR-181a and miR-181b. Additionally, decreased levels of two CLL signature miRNAs miR-29c and miR-223 are associated with ZAP70(+ and IgV(H unmutated status and with shorter time to first therapy. These data indicate an activated B cell status for CLL cells and suggest that the direction of change of individual miRNAs may predict clinical course in CLL.

  2. Measuring Cognitive Load in Test Items: Static Graphics versus Animated Graphics

    Science.gov (United States)

    Dindar, M.; Kabakçi Yurdakul, I.; Inan Dönmez, F.

    2015-01-01

    The majority of multimedia learning studies focus on the use of graphics in learning process but very few of them examine the role of graphics in testing students' knowledge. This study investigates the use of static graphics versus animated graphics in a computer-based English achievement test from a cognitive load theory perspective. Three…

  3. Graphic Presentation: An Empirical Examination of the Graphic Novel Approach to Communicate Business Concepts

    Science.gov (United States)

    Short, Jeremy C.; Randolph-Seng, Brandon; McKenny, Aaron F.

    2013-01-01

    Graphic novels have been increasingly incorporated into business communication forums. Despite potential benefits, little research has examined the merits of the graphic novel approach. In response, we engage in a two-study approach. Study 1 explores the potential of graphic novels to affect learning outcomes and finds that the graphic novel was…

  4. Physical activity profiles and selected muscular fitness variables in English schoolchildren: A north-south divide?

    Science.gov (United States)

    Ingle, Lee; Stephenson, Ashlie; Sandercock, Gavin R

    2016-11-01

    The aim of the study was to compare and contrast habitual physical activity (PA) profiles and muscular fitness in schoolchildren from northern and southern regions of England. Data were collected from two secondary schools in the north east (NE) of England. The study procedures followed methods employed by the East of England Healthy Hearts Study in 10-16-year-old boys and girls based in the south east (SE) region of England and data were compared. Habitual physical activity (PAQ-A), vertical jump test, and hand-grip (HG) strength were assessed. We converted raw scores from all assessments to age- and sex-normalised z-scores. We recruited 597 children (58% boys) in the NE and compared findings to 597 age- and sex-matched boys and girls from the SE. Boys in the SE had significantly stronger HG scores, jumped higher, were more powerful (mean peak power: 2131 W vs. 1782 W; P PAQ-A: 2.9 vs. 2.5; P < 0.0001) than their male counterparts in the NE. In girls, the opposite trend was evident. Girls from the NE of England had a higher HG score, jumped higher, and were more powerful (mean peak power: 2114 W vs. 1839 W; P < .0001) than their peers from the SE. Regional variations in the habitual PA profiles and muscular fitness of schoolchildren from the SE and NE of England do exist. The systematic surveillance of children's PA and fitness profiles throughout England would help identify regional inequalities on a larger scale.

  5. The profile of physical activity and coronary risk factors in Monica Jakarta survey

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    Dede Kusmana

    2001-03-01

    Full Text Available A population study was done to know the profile of daily physical activity including sports, and its influence on major coronary risk factors in three districts of Jakarta using questionnaire, physicaL and laboratory examination, and 12 leads ECC. The questionnaire gave the data about physical activity (work load and sport, and smoking habit. Work load was divided into light, moderate and heavy. Sport activity was divided into regular (≥ twice a week, 20 minutes or more, irregular (≤ once or occasional, and no sport activity. In addition, blood pressure  (hypertension was grouped according to WHO criteria, total cholesterol (regarded as hypercholesterolemia when > 200 mg%, and ECG were measured. ECG interpretation was done using Minnesota Code. Statistical analysis was done using SPSS. Out of 2400 people there were  2073(86.4%  respondents that consist of 1086 females and 987 males. The profile of physical activity as a whole showed 33.4% light, 50.7% moderate and 15.8% heavy activity. OnLy 22.5% of respondents had sport regularly, while 30.3% had sport irregularly, and 47.2% had no sport activity. The type of sport was walking (45.0%, callisthenic (22.0% jogging/running (15.6%, badminton (6.5%, volley ball/soccer (4.1%, tennis (3.8%, and golf (0.1%. There was a significant difference in the prevalence of hypertension between people with heavy (12.0%, moderate (44.8% and light work load (43.2% (P<0.003, in the prevalence of hypercholesterolemia (13.2%: 50.8%: 36% (P<0.0003, and smoking (19.7%; 44.1%: 36.2% (P<0.00001, respectively. The difference also occurred in the prevalence of abnormal ECG (Q/QS, ST and T  abnormalities between people having regular sport (19.0%, irregular (22.7%, and no sport activity (58.3% (P<0.05. The number of respondents having enough physical activity (including regular sport was relatively low. Therefore, promotion should be done as a preventive method to overcome cardiovascular risk factors. (Med J Indones

  6. An exposure:activity profiling method for interpreting high-throughput screening data for estrogenic activity--proof of concept.

    Science.gov (United States)

    Becker, Richard A; Friedman, Katie Paul; Simon, Ted W; Marty, M Sue; Patlewicz, Grace; Rowlands, J Craig

    2015-04-01

    Rapid high throughput in vitro screening (HTS) assays are now available for characterizing dose-responses in assays that have been selected for their sensitivity in detecting estrogen-related endpoints. For example, EPA's ToxCast™ program recently released endocrine assay results for more than 1800 substances and the interagency Tox21 consortium is in the process of releasing data for approximately 10,000 chemicals. But such activity measurements alone fall short for the purposes of priority setting or screening because the relevant exposure context is not considered. Here, we extend the method of exposure:activity profiling by calculating the exposure:activity ratios (EARs) using human exposure estimates and AC50 values for a range of chemicals tested in a suite of seven estrogenic assays in ToxCast™ and Tox21. To provide additional context, relative estrogenic exposure:activity quotients (REEAQ) were derived by comparing chemical-specific EARs to the EAR of the ubiquitous dietary phytoestrogen, genistein (GEN). Although the activity of a substance in HTS-endocrine assays is not a measure of health hazard or risk, understanding how such a dose compares to human exposures provides a valuable additional metric that can be used in decision-making; substances with small EARs and REEAQs would indicate low priority for further endocrine screening or testing.

  7. Antioxidant activity, cytotoxic activity and metabolic profiling of juices obtained from saffron (Crocus sativus L.) floral by-products.

    Science.gov (United States)

    Tuberoso, Carlo I G; Rosa, Antonella; Montoro, Paola; Fenu, Maurizio Antonio; Pizza, Cosimo

    2016-05-15

    Juices obtained from cold-pressed saffron (Crocus sativus L.) floral by-products were evaluated as a potential source of compounds with antioxidant and cytotoxic activities. Floral by-products were split in two batches for extraction 24 and 48h after flower harvesting, respectively. The in vitro anti-oxidant activity of these extracts was tested using the FRAP and DPPH assays, and two biological models of lipid oxidation (activity in preventing cholesterol degradation and protection against Cu(2+)-mediated degradation of the liposomal unsaturated fatty acids). The cytotoxic activity was evaluated using the MTT assay. The results show that extracts obtained 48h post-harvest contained higher levels of total polar phenols and had the highest antioxidant activity in all of the performed assays. The LC-DAD and LC-ESI-(HR)MS(n) metabolic profiles showed high levels of kaempferol derivatives and anthocyanins. This study suggests that juices from saffron floral by-products could potentially be used to develop new products for the food and health industry.

  8. Phenolic profiles and antioxidant and anticarcinogenic activities of Greek herbal infusions; balancing delight and chemoprevention?

    Science.gov (United States)

    Kaliora, Andriana C; Kogiannou, Dimitra A A; Kefalas, Panagiotis; Papassideri, Issidora S; Kalogeropoulos, Nick

    2014-01-01

    Total phenolic content, antioxidant activity and phenolic profiles of six herbal infusions - namely rosemary, Cretan dittany, St. John's Wort, sage, marjoram and thyme were assayed. Additionally, the infusion anticarcinogenic effect as to their ability to (a) scavenge free radicals, (b) inhibit cell growth, (c) decrease IL-8 levels and (d) regulate p65 subunit in epithelial colon cancer (HT29) and prostate (PC3) cancer cells was investigated. LC-DAD-MS and GC-MS analyses showed major qualitative and quantitative differences in phenolic profiles of the infusions. All herbal infusions exhibited antiradical activity which correlated strongly with their total phenolic content. Infusions exhibited the potential to inhibit cell growth and to reduce IL-8 levels in HT29 colon and PC3 prostate cancer cells. The regulation reported in p65 subunit in HT29 treated with St John's Wort and in PC3 treated with thyme might point to the NF-κB as the molecular target underlying the effect of these infusions.

  9. Physiological response and activity profile in recreational small-sided football

    DEFF Research Database (Denmark)

    Randers, Morten Bredsgaard; Nielsen, Jens Jung; Bangsbo, Jens;

    2014-01-01

    We examined the effect of the number of players on the activity profile and physiological response to small-sided recreational football games with fixed relative pitch size. Twelve untrained men (age: 33.0 ± 6.4 (± standard deviation) years, fat%: 22.4 ± 6.1%, VO2 max: 43.3 ± 5.2 mL/min/kg) compl......We examined the effect of the number of players on the activity profile and physiological response to small-sided recreational football games with fixed relative pitch size. Twelve untrained men (age: 33.0 ± 6.4 (± standard deviation) years, fat%: 22.4 ± 6.1%, VO2 max: 43.3 ± 5.2 m...... accelerations (500 ± 139 vs 459 ± 143 and 396 ± 144) were higher (P physiological responses for 6-14 players when pitch size is adapted, providing further evidence...

  10. Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model

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    Ethem Unal

    2012-01-01

    Full Text Available Objective. In the present study, since PON1 is known as an HDL-associated antioxidant enzyme that inhibits the oxidative modification of LDL and oxidative stress plays a role in the pathogenesis of mesenteric ischemia, we investigated the changes in PON1 activity and lipid profile in an experimental ischemic colitis model. Methods. Forty male Wistar albino rats were divided into two groups: the control group (N=15 and the experimental group (N=25. All animals were anesthetized with ether and ketamine anesthesia to undergo a midline laparotomy. Ischemic colitis was induced by marginal vessel ligation in the splenic flexura (devascularization process. A sham laparotomy was performed in the control group. All animals were sacrificed on the seventh postoperative day. Oxidative stress marker (malonyldialdehyde, MDA, lipid profile, and paraoxonase (PON-1 and arylesterase activities were determined. Histopathological evaluation was done under light microscopy, after sectioning and staining with hematoxyline and eosin. Statistical analysis was conducted using Student’s t-test and Mann-Whitney U test, and P0.05. Conclusions. PON1 and arylesterase play an important role in the pathophysiology of ischemic colitis.

  11. Dynamic, large-scale profiling of transcription factor activity from live cells in 3D culture.

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    Michael S Weiss

    Full Text Available BACKGROUND: Extracellular activation of signal transduction pathways and their downstream target transcription factors (TFs are critical regulators of cellular processes and tissue development. The intracellular signaling network is complex, and techniques that quantify the activities of numerous pathways and connect their activities to the resulting phenotype would identify the signals and mechanisms regulating tissue development. The ability to investigate tissue development should capture the dynamic pathway activity and requires an environment that supports cellular organization into structures that mimic in vivo phenotypes. Taken together, our objective was to develop cellular arrays for dynamic, large-scale quantification of TF activity as cells organized into spherical structures within 3D culture. METHODOLOGY/PRINCIPAL FINDINGS: TF-specific and normalization reporter constructs were delivered in parallel to a cellular array containing a well-established breast cancer cell line cultured in Matrigel. Bioluminescence imaging provided a rapid, non-invasive, and sensitive method to quantify luciferase levels, and was applied repeatedly on each sample to monitor dynamic activity. Arrays measuring 28 TFs identified up to 19 active, with 13 factors changing significantly over time. Stimulation of cells with β-estradiol or activin A resulted in differential TF activity profiles evolving from initial stimulation of the ligand. Many TFs changed as expected based on previous reports, yet arrays were able to replicate these results in a single experiment. Additionally, arrays identified TFs that had not previously been linked with activin A. CONCLUSIONS/SIGNIFICANCE: This system provides a method for large-scale, non-invasive, and dynamic quantification of signaling pathway activity as cells organize into structures. The arrays may find utility for investigating mechanisms regulating normal and abnormal tissue growth, biomaterial design, or as a

  12. Antistaphylococcal activity and metabolite profiling of manuka honey (Leptospermum scoparium L.) after in vitro simulated digestion.

    Science.gov (United States)

    Mannina, Luisa; Sobolev, Anatoly P; Coppo, Erika; Di Lorenzo, Arianna; Nabavi, Seyed Mohammad; Marchese, Anna; Daglia, Maria

    2016-03-01

    The antistaphylococcal activity against methicillin-susceptible and -resistant Staphylococcus aureus and the metabolite profiling of manuka honey (MH) were investigated before and after in vitro simulated gastric (GD) and gastroduodenal (GDD) digestions. Undigested manuka honey showed antibacterial activity against all the tested strains, the GD sample showed no activity against S. aureus, and the GDD honey showed an antistaphylococcal activity, which was slightly reduced in comparison with the undigested sample. To explain these results, methylglyoxal (MGO), to which most of the antibacterial activity of MH is ascribed, was subjected to in vitro simulated GD and GDD. After digestion, MGO showed antibacterial activity at concentrations definitively higher than those registered in digested MH samples. These results showed that the antistaphylococcal activity registered after digestion cannot be ascribed to MGO. Thus metabolite analysis, carried out using an explorative untargeted NMR-based approach and a targeted RP-HPLC-PAD-ESI-MSn analysis focused on bio-active substances, was used to highlight the chemical modifications occurring from digestion. The results showed that (1) the level of MGO decreases and (2) the content of aromatic compounds, such as leptosin and methyl syringate, markers of manuka honey, was stable under gastric and gastroduodenal conditions, whereas (3) the levels of acetic and lactic acids increase in particular after gastroduodenal digestion, being 1.5 and 2.8 times higher in GDD-MH than in UND-MH, respectively. Overall, the results obtained from chemical analysis provide at least a partial explanation of the registered antibacterial activity observed after gastroduodenal digestion.

  13. Presence of membranous vesicles in cat seminal plasma: ultrastructural characteristics, protein profile and enzymatic activity.

    Science.gov (United States)

    Polisca, A; Troisi, A; Minelli, A; Bellezza, I; Fontbonne, A; Zelli, R

    2015-02-01

    This study sought to verify the presence of membranous vesicles in cat seminal plasma by means of transmission electron microscopy and to identify protein profile and some of the enzymatic activities associated with these particles. The transmission electron microscopy observations showed the existence of different sized vesicular membranous structures of more or less spherical shape. These vesicles were surrounded by single-, double- or multiple-layered laminar membranes. The vesicle diameters ranged from 16.3 to 387.4 nm, with a mean of 116.5 ± 70.7 nm. Enzyme activity determinations showed the presence of dipeptilpeptidase IV, aminopeptidase, alkaline and acid phosphatase. To our knowledge, this is the first report that identifies and characterizes the membranous vesicles in cat seminal plasma. However, further studies are necessary to identify the exact site of production of these membranous vesicles in the cat male genital tract and to determine their specific roles in the reproductive events of this species.

  14. Gene expression profiling of porcine skeletal muscle in the early recovery phase following acute physical activity

    DEFF Research Database (Denmark)

    Hansen, Jeanette; Conley, Lene; Hedegaard, Jakob

    2012-01-01

    Acute physical activity elicits changes in gene expression in skeletal muscles to promote metabolic changes and to repair exercise-induced muscle injuries. In the present time-course study, pigs were submitted to an acute bout of treadmill running until near exhaustion to determine the impact...... of unaccustomed exercise on global transcriptional profiles in porcine skeletal muscles. Using a combined microarray and candidate gene approach, we identified a suite of genes that are differentially expressed in muscles during postexercise recovery. Several members of the heat shock protein family and proteins...... detected an upregulation of genes that are associated with muscle cell proliferation and differentiation, including MUSTN1, ASB5 and CSRP3, possibly reflecting activation, differentiation and fusion of satellite cells to facilitate repair of muscle damage. In addition, exercise increased expression...

  15. Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria.

    Science.gov (United States)

    Vrancheva, Radka Z; Ivanov, Ivan G; Aneva, Ina Y; Dincheva, Ivayla N; Badjakov, Ilian K; Pavlov, Atanas I

    2016-01-01

    GC-MS analysis of alkaloid profiles of five Fumaria species, naturally grown in Bulgaria (F. officinalis, F. thuretii, F. kralikii, F. rostellata and F. schrammii) and analysis of acetylcholinesterase inhibitory activity of alkaloid extracts were performed. Fourteen isoquinoline alkaloids were identified, with the principle ones being protopine, cryptopine, sinactine, parfumine, fumariline, fumarophycine, and fumaritine. Protopine contents, defined by HPLC analysis varied between 210.6 ± 8.8 μg/g DW (F. schrammii) and 334.5 ± 7.1 μg/g DW. (F. rostellata). While all of the investigated alkaloid extracts significantly inhibited acetylcholinesterase activity, the F. kralikii demonstrated the highest level of inhibition (IC(50) 0.13 ± 0.01 mg extract/mL).

  16. Time place learning and activity profile under constant light and constant dark in zebrafish (Danio rerio).

    Science.gov (United States)

    Moura, Clarissa de Almeida; Lima, Jéssica Polyana da Silva; Silveira, Vanessa Augusta Magalhães; Miguel, Mário André Leocadio; Luchiari, Ana Carolina

    2017-02-20

    The ability to learn about the signs of variability in space and time is known as time place learning (TPL). To adjust their circadian rhythms, animals use stimuli that change regularly, such as the light-dark cycle, temperature, food availability or even social stimuli. Because light-dark cycle is the most important environmental temporal cue, we asked how a diurnal animal would perform TPL if this cue was removed. Zebrafish has been extensively studied in the chronobiology area due to it diurnal chronotype, thus, we studied the effects of constant light and constant dark on the time-place learning and activity profile in zebrafish. Our data show that while under constant light and dark condition zebrafish was not able of TPL, after 30days under the constant conditions, constant light led to higher activity level and less significant (robust) 24h rhythm.

  17. Profiling gene expression induced by protease-activated receptor 2 (PAR2 activation in human kidney cells.

    Directory of Open Access Journals (Sweden)

    Jacky Y Suen

    Full Text Available Protease-Activated Receptor-2 (PAR2 has been implicated through genetic knockout mice with cytokine regulation and arthritis development. Many studies have associated PAR2 with inflammatory conditions (arthritis, airways inflammation, IBD and key events in tumor progression (angiogenesis, metastasis, but they have relied heavily on the use of single agonists to identify physiological roles for PAR2. However such probes are now known not to be highly selective for PAR2, and thus precisely what PAR2 does and what mechanisms of downstream regulation are truly affected remain obscure. Effects of PAR2 activation on gene expression in Human Embryonic Kidney cells (HEK293, a commonly studied cell line in PAR2 research, were investigated here by comparing 19,000 human genes for intersecting up- or down-regulation by both trypsin (an endogenous protease that activates PAR2 and a PAR2 activating hexapeptide (2f-LIGRLO-NH(2. Among 2,500 human genes regulated similarly by both agonists, there were clear associations between PAR2 activation and cellular metabolism (1,000 genes, the cell cycle, the MAPK pathway, HDAC and sirtuin enzymes, inflammatory cytokines, and anti-complement function. PAR-2 activation up-regulated four genes more than 5 fold (DUSP6, WWOX, AREG, SERPINB2 and down-regulated another six genes more than 3 fold (TXNIP, RARG, ITGB4, CTSD, MSC and TM4SF15. Both PAR2 and PAR1 activation resulted in up-regulated expression of several genes (CD44, FOSL1, TNFRSF12A, RAB3A, COPEB, CORO1C, THBS1, SDC4 known to be important in cancer. This is the first widespread profiling of specific activation of PAR2 and provides a valuable platform for better understanding key mechanistic roles of PAR2 in human physiology. Results clearly support the development of both antagonists and agonists of human PAR2 as potential disease modifying therapeutic agents.

  18. Interpretation of pH-activity profiles for acid-base catalysis from molecular simulations.

    Science.gov (United States)

    Dissanayake, Thakshila; Swails, Jason M; Harris, Michael E; Roitberg, Adrian E; York, Darrin M

    2015-02-17

    The measurement of reaction rate as a function of pH provides essential information about mechanism. These rates are sensitive to the pK(a) values of amino acids directly involved in catalysis that are often shifted by the enzyme active site environment. Experimentally observed pH-rate profiles are usually interpreted using simple kinetic models that allow estimation of "apparent pK(a)" values of presumed general acid and base catalysts. One of the underlying assumptions in these models is that the protonation states are uncorrelated. In this work, we introduce the use of constant pH molecular dynamics simulations in explicit solvent (CpHMD) with replica exchange in the pH-dimension (pH-REMD) as a tool to aid in the interpretation of pH-activity data of enzymes and to test the validity of different kinetic models. We apply the methods to RNase A, a prototype acid-base catalyst, to predict the macroscopic and microscopic pK(a) values, as well as the shape of the pH-rate profile. Results for apo and cCMP-bound RNase A agree well with available experimental data and suggest that deprotonation of the general acid and protonation of the general base are not strongly coupled in transphosphorylation and hydrolysis steps. Stronger coupling, however, is predicted for the Lys41 and His119 protonation states in apo RNase A, leading to the requirement for a microscopic kinetic model. This type of analysis may be important for other catalytic systems where the active forms of the implicated general acid and base are oppositely charged and more highly correlated. These results suggest a new way for CpHMD/pH-REMD simulations to bridge the gap with experiments to provide a molecular-level interpretation of pH-activity data in studies of enzyme mechanisms.

  19. A Multi-Agent Mah Jong Playing System: Towards Real-Time Recognition of Graphic Units in Graphic Representations

    Directory of Open Access Journals (Sweden)

    H. Achten

    2003-01-01

    Full Text Available In architectural design, sketching is an important means to explore the first conceptual developments in the design process. It is necessary to understand the conventions of depiction and encoding in sketches and drawings if we want to support the architect in the sketching activity. The theory of graphic units provides a comprehensive list of conventions of depiction and encoding that are widely used among architects. These graphic units form useful building blocks to understand design drawings. We investigate whether it is possible to build a system that can recognize graphic units. The technology we are looking at is multi-agent systems. It was chosen for the following reasons: agents can specialize in graphic units, a multi-agent system can deal with ambiguity through negotiation and conflict resolution, and multi-agent systems function in dynamically changing environments. Currently there is no general approach or technology available for multi-agent systems. Therefore, in our research we first set out to make such a multi-agent system. In order to keep the complexity low, we first aim to make a system that can do something simple: playing Mah Jong solitary. The Mah Jong solitary system shares the following important features with a multi-agent system that can recognize graphic units: (1 specialized agents for moves; (2 negotiation between agents to establish the best move; (3 a dynamically changing environment; and (4 search activity for more advanced strategies. The paper presents the theoretical basis of graphic units and multi-agents systems, followed by a description of the multi-agent framework and its implementation. A number of systems that can play Mah Jong at various degrees of competence and accordingly degrees of complexity of multi-agent system, are distinguished. Finally, the paper demonstrates how the findings are informative for a system that can recognize graphic units.

  20. Biological activities and phytochemical profiles of extracts from different parts of bamboo (Phyllostachys pubescens).

    Science.gov (United States)

    Tanaka, Akinobu; Zhu, Qinchang; Tan, Hui; Horiba, Hiroki; Ohnuki, Koichiro; Mori, Yasuhiro; Yamauchi, Ryoko; Ishikawa, Hiroya; Iwamoto, Akira; Kawahara, Hiroharu; Shimizu, Kuniyoshi

    2014-06-18

    Besides being a useful building material, bamboo also is a potential source of bioactive substances. Although some studies have been performed to examine its use in terms of the biological activity, only certain parts of bamboo, especially the leaves or shoots, have been studied. Comprehensive and comparative studies among different parts of bamboo would contribute to a better understanding and application of this knowledge. In this study, the biological activities of ethanol and water extracts from the leaves, branches, outer culm, inner culm, knots, rhizomes and roots of Phyllostachys pubescens, the major species of bamboo in Japan, were comparatively evaluated. The phytochemical profiles of these extracts were tentatively determined by liquid chromatography-mass spectrometry (LC-MS) analysis. The results showed that extracts from different parts of bamboo had different chemical compositions and different antioxidative, antibacterial and antiallergic activities, as well as on on melanin biosynthesis. Outer culm and inner culm were found to be the most important sources of active compounds. 8-C-Glucosylapigenin, luteolin derivatives and chlorogenic acid were the most probable compounds responsible for the anti-allergy activity of these bamboo extracts. Our study suggests the potential use of bamboo as a functional ingredient in cosmetics or other health-related products.

  1. Antioxidant activity of some Moroccan marine microalgae: Pufa profiles, carotenoids and phenolic content.

    Science.gov (United States)

    Maadane, Amal; Merghoub, Nawal; Ainane, Tarik; El Arroussi, Hicham; Benhima, Redouane; Amzazi, Saaid; Bakri, Youssef; Wahby, Imane

    2015-12-10

    In order to promote Moroccan natural resources, this study aims to evaluate the potential of microalgae isolated from Moroccan coastlines, as new source of natural antioxidants. Different extracts (ethanolic, ethanol/water and aqueous) obtained from 9 microalgae strains were screened for their in vitro antioxidant activity using DPPH free radical-scavenging assay. The highest antioxidant potentials were obtained in Dunalliela sp., Tetraselmis sp. and Nannochloropsis gaditana extracts. The obtained results indicate that ethanol extract of all microalgae strains exhibit higher antioxidant activity, when compared to water and ethanol/water extracts. Therefore, total phenolic and carotenoid content measurement were performed in active ethanol extracts. The PUFA profiles of ethanol extracts were also determined by GC/MS analysis. The studied microalgae strains displayed high PUFA content ranging from 12.9 to 76.9 %, total carotenoids content varied from 1.9 and 10.8mg/g of extract and total polyphenol content varied from 8.1 to 32.0mg Gallic acid Equivalent/g of extract weight. The correlation between the antioxidant capacities and the phenolic content and the carotenoids content were found to be insignificant, indicating that these compounds might not be major contributor to the antioxidant activity of these microalgae. The microalgae extracts exerting the high antioxidant activity are potential new source of natural antioxidants.

  2. Biological Activities and Phytochemical Profiles of Extracts from Different Parts of Bamboo (Phyllostachys pubescens

    Directory of Open Access Journals (Sweden)

    Akinobu Tanaka

    2014-06-01

    Full Text Available Besides being a useful building material, bamboo also is a potential source of bioactive substances. Although some studies have been performed to examine its use in terms of the biological activity, only certain parts of bamboo, especially the leaves or shoots, have been studied. Comprehensive and comparative studies among different parts of bamboo would contribute to a better understanding and application of this knowledge. In this study, the biological activities of ethanol and water extracts from the leaves, branches, outer culm, inner culm, knots, rhizomes and roots of Phyllostachys pubescens, the major species of bamboo in Japan, were comparatively evaluated. The phytochemical profiles of these extracts were tentatively determined by liquid chromatography-mass spectrometry (LC-MS analysis. The results showed that extracts from different parts of bamboo had different chemical compositions and different antioxidative, antibacterial and antiallergic activities, as well as on on melanin biosynthesis. Outer culm and inner culm were found to be the most important sources of active compounds. 8-C-Glucosylapigenin, luteolin derivatives and chlorogenic acid were the most probable compounds responsible for the anti-allergy activity of these bamboo extracts. Our study suggests the potential use of bamboo as a functional ingredient in cosmetics or other health-related products.

  3. Active faults in the deformation zone off Noto Peninsula, Japan, revealed by high- resolution seismic profiles

    Science.gov (United States)

    Inoue, T.; Okamura, Y.; Murakami, F.; Kimura, H.; Ikehara, K.

    2008-12-01

    Recently, a lot of earthquakes occur in Japan. The deformation zone which many faults and folds have concentrated exists on the Japan Sea side of Japan. The 2007 Noto Hanto Earthquake (MJMA 6.9) and 2007 Chuetsu-oki Earthquake (MJMA 6.8) were caused by activity of parts of faults in this deformation zone. The Noto Hanto Earthquake occurred on 25 March, 2007 under the northwestern coast of Noto Peninsula, Ishikawa Prefecture, Japan. This earthquake is located in Quaternary deformation zone that is continued from northern margin of Noto Peninsula to southeast direction (Okamura, 2007a). National Institute of Advanced Industrial Science and Technology (AIST) carried out high-resolution seismic survey using Boomer and 12 channels short streamer cable in the northern part off Noto Peninsula, in order to clarify distribution and activities of active faults in the deformation zone. A twelve channels short streamer cable with 2.5 meter channel spacing developed by AIST and private corporation is designed to get high resolution seismic profiles in shallow sea area. The multi-channel system is possible to equip on a small fishing boat, because the data acquisition system is based on PC and the length of the cable is short and easy to handle. Moreover, because the channel spacing is short, this cable is very effective for a high- resolution seismic profiling survey in the shallow sea, and seismic data obtained by multi-channel cable can be improved by velocity analysis and CDP stack. In the northern part off Noto Peninsula, seismic profiles depicting geologic structure up to 100 meters deep under sea floor were obtained. The most remarkable reflection surface recognized in the seismic profiles is erosion surface at the Last Glacial Maximum (LGM). In the western part, sediments about 30 meters (40 msec) thick cover the erosional surface that is distributed under the shelf shallower than 100m in depth and the sediments thin toward offshore and east. Flexures like deformation in

  4. Wang Tiles in Computer Graphics

    CERN Document Server

    Lagae, Ares

    2009-01-01

    Many complex signals in computer graphics, such as point distributions and textures, cannot be efficiently synthesized and stored. This book presents tile-based methods based on Wang tiles and corner tiles to solve both these problems. Instead of synthesizing a complex signal when needed, the signal is synthesized beforehand over a small set of Wang tiles or corner tiles. Arbitrary large amounts of that signal can then efficiently be generated when needed by generating a stochastic tiling, and storing only a small set of tiles reduces storage requirements. A tile-based method for generating a

  5. Selective androgen receptor modulator activity of a steroidal antiandrogen TSAA-291 and its cofactor recruitment profile.

    Science.gov (United States)

    Hikichi, Yukiko; Yamaoka, Masuo; Kusaka, Masami; Hara, Takahito

    2015-10-15

    Selective androgen receptor modulators (SARMs) specifically bind to the androgen receptor and exert agonistic or antagonistic effects on target organs. In this study, we investigated the SARM activity of TSAA-291, previously known as a steroidal antiandrogen, in mice because TSAA-291 was found to possess partial androgen receptor agonist activity in reporter assays. In addition, to clarify the mechanism underlying its tissue selectivity, we performed comprehensive cofactor recruitment analysis of androgen receptor using TSAA-291 and dihydrotestosterone (DHT), an endogenous androgen. The androgen receptor agonistic activity of TSAA-291 was more obvious in reporter assays using skeletal muscle cells than in those using prostate cells. In castrated mice, TSAA-291 increased the weight of the levator ani muscle without increasing the weight of the prostate and seminal vesicle. Comprehensive cofactor recruitment analysis via mammalian two-hybrid methods revealed that among a total of 112 cofactors, 12 cofactors including the protein inhibitor of activated STAT 1 (PIAS1) were differently recruited to androgen receptor in the presence of TSAA-291 and DHT. Prostate displayed higher PIAS1 expression than skeletal muscle. Forced expression of the PIAS1 augmented the transcriptional activity of the androgen receptor, and silencing of PIAS1 by siRNAs suppressed the secretion of prostate-specific antigen, an androgen responsive marker. Our results demonstrate that TSAA-291 has SARM activity and suggest that TSAA-291 may induce different conformational changes of the androgen receptor and recruitment profiles of cofactors such as PIAS1, compared with DHT, to exert tissue-specific activity.

  6. Gene expression profiling of aging reveals activation of a p53-mediated transcriptional program

    Directory of Open Access Journals (Sweden)

    Weindruch Richard

    2007-03-01

    Full Text Available Abstract Background Aging has been associated with widespread changes at the gene expression level in multiple mammalian tissues. We have used high density oligonucleotide arrays and novel statistical methods to identify specific transcriptional classes that may uncover biological processes that play a central role in mammalian aging. Results We identified 712 transcripts that are differentially expressed in young (5 month old and old (25-month old mouse skeletal muscle. Caloric restriction (CR completely or partially reversed 87% of the changes in expression. Examination of individual genes revealed a transcriptional profile indicative of increased p53 activity in the older muscle. To determine whether the increase in p53 activity is associated with transcriptional activation of apoptotic targets, we performed RT-PCR on four well known mediators of p53-induced apoptosis: puma, noxa, tnfrsf10b and bok. Expression levels for these proapoptotic genes increased significantly with age (P +/- and GPX4+/- mice, suggesting that oxidative stress does not induce the expression of these genes. Western blot analysis confirmed that protein levels for both p21 and GADD45a, two established transcriptional targets of p53, were higher in the older muscle tissue. Conclusion These observations support a role for p53-mediated transcriptional program in mammalian aging and suggest that mechanisms other than reactive oxygen species are involved in the age-related transcriptional activation of p53 targets.

  7. Phytochemical Profiles and Antioxidant and Antimicrobial Activities of the Leaves of Zanthoxylum bungeanum

    Directory of Open Access Journals (Sweden)

    Yujuan Zhang

    2014-01-01

    Full Text Available The ethanol crude extracts (ECE and their subfractions from Zanthoxylum bungeanum leaves were prepared and their phytochemical profiles and antioxidant and antimicrobial activities were investigated. Moreover, the effective HPLC procedure for simultaneous quantification of twelve compounds in Z. bungeanum leaves was established. The correlation between the phytochemicals and antioxidant activity was also discussed. The ethyl acetate fraction (EAF had the highest total phenolic (97.29 mmol GAE/100 g and flavonoid content (67.93 mmol QE/100 g, while the greatest total alkaloid content (4.39 mmol GAE/100 g was observed in the chloroform fraction (CF. Twelve compounds were quantified by RP-HPLC assay. EAF exhibited the highest content of quercitrin, kaempferol-3-rhamnoside, quercetin, sesamin, and nitidine chloride (125.21, 54.95, 24.36, 26.24, and 0.20 mg/g; acetone fraction (AF contained the highest content of chlorogenic acid, rutin, hyperoside, and trifolin (5.87, 29.94, 98.33, and 31.24 mg/g, while kaempferol-3-rhamnoside, xanthyletin, and sesamin were rich in CF. EAF and AF exhibited significant DPPH, ABTS radical scavenging abilities and reducing power (FRAP, whereas CF exhibited significant antifungal activity. Moreover, EAF also showed stronger antibacterial activity. In conclusion, Z. bungeanum leaves have health benefits when consumed and could be served as an accessible source for production of functional food ingredients and medicinal exploration.

  8. Variability in HOMA-IR, lipoprotein profile and selected hormones in young active men.

    Science.gov (United States)

    Keska, Anna; Lutoslawska, Grazyna; Czajkowska, Anna; Tkaczyk, Joanna; Mazurek, Krzysztof

    2013-01-01

    Resistance to insulin actions is contributing to many metabolic disturbances. Such factors as age, sex, nutrition, body fat, and physical activity determine body insulin resistance. Present study attempted to asses insulin resistance and its metabolic effects with respect to energy intake in young, lean, and active men. A total of 87 men aged 18-23 participated in the study. Plasma levels of glucose, insulin, lipoproteins, cortisol, and TSH were determined. Insulin resistance was expressed as Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) and calculated using homeostatic model. The median value of HOMA-IR (1.344) was used to divide subjects into two groups. Men did not differ in anthropometric parameters, daily physical activity, and plasma TSH and cortisol levels. However, in men with higher HOMA-IR significantly lower daily energy intake was observed concomitantly with higher TG, TC, and HDL-C concentrations in plasma versus their counterparts with lower HOMA-IR. Exclusively in subjects with higher HOMA-IR significant and positive correlation was noted between HOMA-IR and TC and LDL-C. We concluded that despite a normal body weight and physical activity, a subset of young men displayed unfavorable changes in insulin sensitivity and lipid profile, probably due to insufficient energy intake.

  9. Variability in HOMA-IR, Lipoprotein Profile and Selected Hormones in Young Active Men

    Directory of Open Access Journals (Sweden)

    Anna Keska

    2013-01-01

    Full Text Available Resistance to insulin actions is contributing to many metabolic disturbances. Such factors as age, sex, nutrition, body fat, and physical activity determine body insulin resistance. Present study attempted to asses insulin resistance and its metabolic effects with respect to energy intake in young, lean, and active men. A total of 87 men aged 18–23 participated in the study. Plasma levels of glucose, insulin, lipoproteins, cortisol, and TSH were determined. Insulin resistance was expressed as Homeostasis Model Assessment for Insulin Resistance (HOMA-IR and calculated using homeostatic model. The median value of HOMA-IR (1.344 was used to divide subjects into two groups. Men did not differ in anthropometric parameters, daily physical activity, and plasma TSH and cortisol levels. However, in men with higher HOMA-IR significantly lower daily energy intake was observed concomitantly with higher TG, TC, and HDL-C concentrations in plasma versus their counterparts with lower HOMA-IR. Exclusively in subjects with higher HOMA-IR significant and positive correlation was noted between HOMA-IR and TC and LDL-C. We concluded that despite a normal body weight and physical activity, a subset of young men displayed unfavorable changes in insulin sensitivity and lipid profile, probably due to insufficient energy intake.

  10. Systemic Immune Activation Profiles of HIV-1 Subtype C-Infected Children and Their Mothers

    Directory of Open Access Journals (Sweden)

    Tinyiko G. Makhubele

    2016-01-01

    Full Text Available Little is known about immune activation profiles of children infected with HIV-1 subtype C. The current study compared levels of selected circulating biomarkers of immune activation in HIV-1 subtype C-infected untreated mothers and their children with those of healthy controls. Multiplex bead array, ELISA, and immunonephelometric procedures were used to measure soluble CD14 (sCD14, beta-2 microglobulin (β2M, CRP, MIG, IP-10, and transforming growth factor beta 1 (TGF-β1. Levels of all 6 biomarkers were significantly elevated in the HIV-infected mothers and, with the exception of MIG, in their children (P<0.01–P<0.0001. The effects of antiretroviral therapy (ART and maternal smoking on these biomarkers were also assessed. With the exception of TGF-β1, which was unchanged in the children 12 months after therapy, initiation of ART was accompanied by decreases in the other biomarkers. Regression analysis revealed that although most biomarkers were apparently unaffected by smoking, exposure of children to maternal smoking was associated with a significant increase in IP-10. These findings demonstrate that biomarkers of immune activation are elevated in HIV-infected children pre-ART and decline, with the exception of TGF-β1, after therapy. Although preliminary, elevation of IP-10 in smoke-exposed infants is consistent with a higher level of immune activation in this group.

  11. Approximate Counting of Graphical Realizations.

    Science.gov (United States)

    Erdős, Péter L; Kiss, Sándor Z; Miklós, István; Soukup, Lajos

    2015-01-01

    In 1999 Kannan, Tetali and Vempala proposed a MCMC method to uniformly sample all possible realizations of a given graphical degree sequence and conjectured its rapidly mixing nature. Recently their conjecture was proved affirmative for regular graphs (by Cooper, Dyer and Greenhill, 2007), for regular directed graphs (by Greenhill, 2011) and for half-regular bipartite graphs (by Miklós, Erdős and Soukup, 2013). Several heuristics on counting the number of possible realizations exist (via sampling processes), and while they work well in practice, so far no approximation guarantees exist for such an approach. This paper is the first to develop a method for counting realizations with provable approximation guarantee. In fact, we solve a slightly more general problem; besides the graphical degree sequence a small set of forbidden edges is also given. We show that for the general problem (which contains the Greenhill problem and the Miklós, Erdős and Soukup problem as special cases) the derived MCMC process is rapidly mixing. Further, we show that this new problem is self-reducible therefore it provides a fully polynomial randomized approximation scheme (a.k.a. FPRAS) for counting of all realizations.

  12. Approximate Counting of Graphical Realizations.

    Directory of Open Access Journals (Sweden)

    Péter L Erdős

    Full Text Available In 1999 Kannan, Tetali and Vempala proposed a MCMC method to uniformly sample all possible realizations of a given graphical degree sequence and conjectured its rapidly mixing nature. Recently their conjecture was proved affirmative for regular graphs (by Cooper, Dyer and Greenhill, 2007, for regular directed graphs (by Greenhill, 2011 and for half-regular bipartite graphs (by Miklós, Erdős and Soukup, 2013. Several heuristics on counting the number of possible realizations exist (via sampling processes, and while they work well in practice, so far no approximation guarantees exist for such an approach. This paper is the first to develop a method for counting realizations with provable approximation guarantee. In fact, we solve a slightly more general problem; besides the graphical degree sequence a small set of forbidden edges is also given. We show that for the general problem (which contains the Greenhill problem and the Miklós, Erdős and Soukup problem as special cases the derived MCMC process is rapidly mixing. Further, we show that this new problem is self-reducible therefore it provides a fully polynomial randomized approximation scheme (a.k.a. FPRAS for counting of all realizations.

  13. Visualization of exploration data and computer graphics. Chikyukai wo kashikasuru sochi to giho (computer graphics)

    Energy Technology Data Exchange (ETDEWEB)

    Ota, K.; Tuchiya, H. (OYO Corp., Tokyo (Japan))

    1994-05-01

    In relation to how to visualize the underground, the present report introduces computer graphic (CG) devices easily usable by site technicians. Examples of utilizing them are also explained. A printer connected to the personal computer or EWS by interface is outlined together with the operational principle of printer and plotter, and points to which attention must be paid for structuring them as a system. Also, exhibited is an imaging system connected to the minicomputer or EWS. Then, an example of indicating, with an accentuation by CG expression, the characteristics of underground radar survey data is introduced together with another one of outputting, by two types of plotter, the seabed surface survey data by side scan sonar. As for the geological profile preparation system by CG technology, its structure of both software and hardware is outlined together with its system function and examples of utilization giving its excellent system effect. 8 refs., 3 figs., 2 tabs.

  14. Effect of Physical Activity on Anxiety and Depression Among Women: Risk Profiles

    Directory of Open Access Journals (Sweden)

    Aurelio Olmedilla-Zafra**

    2009-12-01

    Full Text Available Scientific literature indicates that there is a positive relationship between exercise and various psychological aspects such as anxiety and depression. Some sociodemographic variables also seem to be involved in this relationship, and socio-cultural factors can be important. The objectives of the present study were to find out the influence of the practice of physical activity on anxiety and depression and to determine the profiles at greater risk with regard to certain sociodemographic variables (age, level of studies, occupation, and children. The sample was composed of 200 women from the city of Cartagena, Spain, between 18 and 65 years of age. Results show that the most vulnerable groups of women for suffering greater levels of anxiety and depression are characterized by the following profiles: anxiety (sedentary women, between 18-24 years of age or older than 54 years, who have not completed studies or those with secondary studies, housewives or those who work outside the home, and those without children and depression (sedentary women, older than 54 years of age or between 18-24 years, those with secondary studies, and housewives.

  15. Protein Profiling and Histone Deacetylation Activities in Somaclonal Variants of Oil Palm (Elaeis guineensis Jacq.

    Directory of Open Access Journals (Sweden)

    Jamilah Syafawati Yaacob

    2013-01-01

    Full Text Available Mantled fruits as a result of somaclonal variation are often observed from the oil palm plantlets regenerated via tissue culture. The mantling of fruits with finger-like and thick outer coating phenotypes significantly reduces the seed size and oil content, posing a threat to oil palm planters, and may jeopardize the economic growth of countries that depend particularly on oil palm plantation. The molecular aspects of the occurrence of somaclonal variations are yet to be known, possibly due to gene repression such as DNA methylation, histone methylation and histone deacetylation. Histone deacetylases (HDACs, involved in eukaryotic gene regulation by catalyzing the acetyl groups are removal from lysine residues on histone, hence transcriptionally repress gene expression. This paper described the total protein polymorphism profiles of somaclonal variants of oil palm and the effects of histone deacetylation on this phenomenon. Parallel to the different phenotypes, the protein polymorphism profiles of the mantled samples (leaves, fruits, and florets and the phenotypically normal samples were proven to be different. Higher HDAC activity was found in mantled leaf samples than in the phenotypically normal leaf samples, leading to a preliminary conclusion that histone deacetylation suppressed gene expression and contributed to the development of somaclonal variants.

  16. Metabolic Profiles and Free Radical Scavenging Activity of Cordyceps bassiana Fruiting Bodies According to Developmental Stage

    Science.gov (United States)

    Hyun, Sun-Hee; Lee, Seok-Young; Sung, Gi-Ho; Kim, Seong Hwan; Choi, Hyung-Kyoon

    2013-01-01

    The metabolic profiles of Cordyceps bassiana according to fruiting body developmental stage were investigated using gas chromatography-mass spectrometry. We were able to detect 62 metabolites, including 48 metabolites from 70% methanol extracts and 14 metabolites from 100% n-hexane extracts. These metabolites were classified as alcohols, amino acids, organic acids, phosphoric acids, purine nucleosides and bases, sugars, saturated fatty acids, unsaturated fatty acids, or fatty amides. Significant changes in metabolite levels were found according to developmental stage. Relative levels of amino acids, purine nucleosides, and sugars were higher in development stage 3 than in the other stages. Among the amino acids, valine, isoleucine, lysine, histidine, glutamine, and aspartic acid, which are associated with ABC transporters and aminoacyl-tRNA biosynthesis, also showed higher levels in stage 3 samples. The free radical scavenging activities, which were significantly higher in stage 3 than in the other stages, showed a positive correlation with purine nucleoside metabolites such as adenosine, guanosine, and inosine. These results not only show metabolic profiles, but also suggest the metabolic pathways associated with fruiting body development stages in cultivated C. bassiana. PMID:24058459

  17. Metabolic profiles and free radical scavenging activity of Cordyceps bassiana fruiting bodies according to developmental stage.

    Directory of Open Access Journals (Sweden)

    Sun-Hee Hyun

    Full Text Available The metabolic profiles of Cordyceps bassiana according to fruiting body developmental stage were investigated using gas chromatography-mass spectrometry. We were able to detect 62 metabolites, including 48 metabolites from 70% methanol extracts and 14 metabolites from 100% n-hexane extracts. These metabolites were classified as alcohols, amino acids, organic acids, phosphoric acids, purine nucleosides and bases, sugars, saturated fatty acids, unsaturated fatty acids, or fatty amides. Significant changes in metabolite levels were found according to developmental stage. Relative levels of amino acids, purine nucleosides, and sugars were higher in development stage 3 than in the other stages. Among the amino acids, valine, isoleucine, lysine, histidine, glutamine, and aspartic acid, which are associated with ABC transporters and aminoacyl-tRNA biosynthesis, also showed higher levels in stage 3 samples. The free radical scavenging activities, which were significantly higher in stage 3 than in the other stages, showed a positive correlation with purine nucleoside metabolites such as adenosine, guanosine, and inosine. These results not only show metabolic profiles, but also suggest the metabolic pathways associated with fruiting body development stages in cultivated C. bassiana.

  18. Computer graphics techniques and computer-generated movies

    Science.gov (United States)

    Holzman, Robert E.; Blinn, James F.

    1988-04-01

    The JPL Computer Graphics Laboratory (CGL) has been using advanced computer graphics for more than ten years to simulate space missions and related activities. Applications have ranged from basic computer graphics used interactively to allow engineers to study problems, to sophisticated color graphics used to simulate missions and produce realistic animations and stills for use by NASA and the scientific press. In addition, the CGL did the computer animation for ``Cosmos'', a series of general science programs done for Public Television in the United States by Carl Sagan and shown world-wide. The CGL recently completed the computer animation for ``The Mechanical Universe'', a series of fifty-two half-hour elementary physics lectures, led by Professor David Goodstein of the California Institute of Technology, and now being shown on Public Television in the US. For this series, the CGL produced more than seven hours of computer animation, averaging approximately eight minutes and thirty seconds of computer animation per half-hour program. Our aim at the JPL Computer Graphics Laboratory (CGL) is the realistic depiction of physical phenomena, that is, we deal primarily in ``science education'' rather than in scientific research. Of course, our attempts to render physical events realistically often require the development of new capabilities through research or technology advances, but those advances are not our primary goal.

  19. Engineering graphic modelling a workbook for design engineers

    CERN Document Server

    Tjalve, E; Frackmann Schmidt, F

    2013-01-01

    Engineering Graphic Modelling: A Practical Guide to Drawing and Design covers how engineering drawing relates to the design activity. The book describes modeled properties, such as the function, structure, form, material, dimension, and surface, as well as the coordinates, symbols, and types of projection of the drawing code. The text provides drawing techniques, such as freehand sketching, bold freehand drawing, drawing with a straightedge, a draughting machine or a plotter, and use of templates, and then describes the types of drawing. Graphic designers, design engineers, mechanical engine

  20. Helping Students Test Programs That Have Graphical User Interfaces

    Directory of Open Access Journals (Sweden)

    Matthew Thornton

    2008-08-01

    Full Text Available Within computer science education, many educators are incorporating software testing activities into regular programming assignments. Tools like JUnit and its relatives make software testing tasks much easier, bringing them into the realm of even introductory students. At the same time, many introductory programming courses are now including graphical interfaces as part of student assignments to improve student interest and engagement. Unfortunately, writing software tests for programs that have significant graphical user interfaces is beyond the skills of typical students (and many educators. This paper presents initial work at combining educationally oriented and open-source tools to create an infrastructure for writing tests for Java programs that have graphical user interfaces. Critically, these tools are intended to be appropriate for introductory (CS1/CS2 student use, and to dovetail with current teaching approaches that incorporate software testing in programming assignments. We also include in our findings our proposed approach to evaluating our techniques.

  1. Multi-elemental profile of some Brazilian make-up products by instrumental neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dalmazio, Ilza; Menezes, Maria Angela de B.C., E-mail: id@cdtn.b, E-mail: menezes@cdtn.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Servico de Reator e Tecnicas Analiticas. Lab. de Ativacao Neutronica

    2011-07-01

    Recent works have shown that analysis in cosmetics and beauty products from the European and Asian markets indicate the presence of U, Th and rare earths besides other trace elements. Considering these previous findings and health issues, it would be valuable to obtain information on elements in cosmetics available in the Brazilian market. The purpose of this study was to acquire a multi-elemental profile of some Brazilian make-up products of diverse brands. Samples of eye shadow, liquid base, facial concealer, lipstick, and compact face powder were analyzed applying neutron activation analysis, k{sub 0}-standardization method at CDTN/CNEN, using the TRIGA Mark I IPR-R1 research reactor. Concentrations of more than 30 elements in samples are presented and it was found elements included in Brazilian National Health Surveillance Agency prohibitive list, rare earths, Th and U in a minimum of two cosmetic samples. (author)

  2. Effect of seafood mediated PCB exposure on desaturase activity and PUFA profile in Faroese septuagenarians

    DEFF Research Database (Denmark)

    Tøttenborg, Sandra Søgaard; Choi, Anna L; Bjerve, Kristian S;

    2015-01-01

    Polychlorinated biphenyl (PCB) exposure may affect serum concentrations of polyunsaturated fatty acids (PUFAs) by inhibiting desaturases ∆5 and ∆6 that drive their synthesis from precursor fatty acids. Such changes in the composition of fatty acids may affect cardiovascular disease risk, which...... is thought to increase at elevated PCB exposures. This population-based cross-sectional study examined 712 Faroese men and women aged 70-74 years. The serum phospholipid fraction of fasting blood samples was used to determine the PUFA profile, including linoleic acid, dihomo-γ-linolenic acid, arachidonic...... acid, eicosatrienoic acid, and other relevant fatty acids. Ratios between precursor and metabolite fatty acids were used as proxies for ∆5 and ∆6 desaturase activity. Tertiles of serum-PCB concentrations were used in multiple regression analyses to determine the association between the exposure...

  3. Lipidic profile and the level of physical activity of adolescent scholars - doi:10.5020/18061230.2011.p384

    Directory of Open Access Journals (Sweden)

    Renato Canevari Dutra da Silva

    2012-01-01

    Full Text Available Objective: To assess the relationship between lipid profile and physical activity level of adolescent students in Rio Verde-GO, Brazil. Methods: A descriptive and cross-sectional study, conducted in 2006, with a population comprised by 1,229 adolescent students of both genders, aged 15 to 17 years (X = 15.9 years, SD + 0.81, from public and private schools. The level of physical activity was assessed through the International Physical Activity Questionnaire (IPAQ. Later, 48 teenagers underwent a lipidogram (lipid profile. Lipid concentrations of total cholesterol (TC, HDL-c (high density lipoprotein and LDL (low density lipoprotein and triglycerides (TGL were determined and assessed according to cutoff points proposed by the III Brazilian Guidelines on dyslipidemias and Guideline of Atherosclerosis, Department of Atherosclerosis of Brazilian Society of Cardiology. Statistical analysis was performed by binomial test for proportions and Pearson’s correlation test, adopting p <0.05. Results: Applying IPAQ we found apercentage of 77.7% active adolescents and 22.3% of insufficiently active adolescents, with the highest percentage of active teens in males (p = 0.0000. Adolescents of both sexes from public network were considered more active than teens from private schools. The lipid profile of the studied adolescents was within normal range. Conclusion: There was no relationship between physical activity level and lipid profile of the adolescents assessed.

  4. Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity

    Directory of Open Access Journals (Sweden)

    Marialice Pinto Coelho Silvestre

    2012-12-01

    Full Text Available The aim of this study was to prepare enzymatic hydrolysates from whey protein concentrate with a nutritionally adequate peptide profile and the ability to inhibit angiotensin-converting enzyme (ACE activity. The effects of the type of enzyme used (pancreatin or papain, the enzyme:substrate ratio (E:S ratio=0.5:100, 1:100, 2:100 and 3:100 and the use of ultrafiltration (UF were investigated. The fractionation of peptides was performed by size-exclusion-HPLC, and the quantification of the components of the chromatographic fractions was carried out by a rapid Corrected Fraction Area method. The ACE inhibitory activity (ACE-IA was determined by Reverse Phase-HPLC. All parameters tested affected both the peptide profile and the ACE-IA. The best peptide profile was achieved for the hydrolysates obtained with papain, whereas pancreatin was more advantageous in terms of ACE-IA. The beneficial effect of using a lower E:S ratio on the peptide profile and ACE-IA was observed for both enzymes depending on the conditions used to prepare the hydrolysates. The beneficial effect of not using UF on the peptide profile was observed in some cases for pancreatin and papain. However, the absence of UF yielded greater ACE-IA only when using papain.O objetivo deste estudo foi preparar hidrolisados enzimáticos do concentrado proteico do soro de leite com perfil peptídico nutricionalmente adequado e com capacidade para inibir a atividade da enzima conversora da angiotensina (ECA. Os efeitos do tipo de enzima usado (pancreatina ou papaína, da relação enzima:substrato (E:S=0,5:100, 1:100, 2:100 e 3:100 e do uso da ultrafiltração (UF foram investigados. O fracionamento dos peptídeos foi feito por CLAE de exclusão molecular e a quantificação dos componentes das frações cromatográficas foi realizada pelo método da Área Corrigida da Fração. A atividade inibitória da ECA (AI-ECA foi determinada por CLAE de fase reversa. Todos os parâmetros testados afetaram

  5. Retrieval of Precipitation Profiles from Multiresolution, Multifrequency, Active and Passive Microwave Observations

    Science.gov (United States)

    Grecu, Mircea; Olson, William S.; Anagnostou, Emmanouil N.

    2003-01-01

    In this study, a technique for estimating vertical profiles of precipitation from multifrequency, multiresolution active and passive microwave observations is investigated. The technique is applicable to the Tropical Rainfall Measuring Mission (TRMM) observations and it is based on models that simulate high-resolution brightness temperatures as functions of observed reflectivity profiles and a parameter related to the rain drop-size-distribution. The modeled high-resolution brightness temperatures are used to determine normalized brightness temperature polarizations at the microwave radiometer resolution. An optimal estimation procedure is employed to minimize the differences between the simulated and observed normalized polarizations by adjusting the drop-size-distribution parameter. The impact of other unknowns that are not independent variables in the optimal estimation but affect the retrievals is minimized through statistical parameterizations derived from cloud model simulations. The retrieval technique is investigated using TRMM observations collected during the Kwajalein Experiment (KWAJEX). These observations cover an area extending from 5 deg to deg N latitude and 166 deg to 172 deg E longitude from July to September 1999, and are coincident with various ground-based observations, facilitating a detailed analysis of the retrieved precipitation. Using the method developed in this study, precipitation estimates consistent with both the passive and active TRMM observations are obtained. Various parameters characterizing these estimates, i.e. the rain rate, the precipitation water content, the drop-size-distribution intercept, and the mass weighted mean drop diameter, are in good qualitative agreement with independent experimental and theoretical estimates. Combined rain estimates are in general higher than the official TRMM Precipitation Radar (PR) only estimates for the area and the period considered in the study. Ground-based precipitation estimates

  6. Development and validation of a method for profiling post-translational modification activities using protein microarrays.

    Directory of Open Access Journals (Sweden)

    Sonia V Del Rincón

    Full Text Available BACKGROUND: Post-translational modifications (PTMs impact on the stability, cellular location, and function of a protein thereby achieving a greater functional diversity of the proteome. To fully appreciate how PTMs modulate signaling networks, proteome-wide studies are necessary. However, the evaluation of PTMs on a proteome-wide scale has proven to be technically difficult. To facilitate these analyses we have developed a protein microarray-based assay that is capable of profiling PTM activities in complex biological mixtures such as whole-cell extracts and pathological specimens. METHODOLOGY/PRINCIPAL FINDINGS: In our assay, protein microarrays serve as a substrate platform for in vitro enzymatic reactions in which a recombinant ligase, or extracts prepared from whole cells or a pathological specimen is overlaid. The reactions include labeled modifiers (e.g., ubiquitin, SUMO1, or NEDD8, ATP regenerating system, and other required components (depending on the assay that support the conjugation of the modifier. In this report, we apply this methodology to profile three molecularly complex PTMs (ubiquitylation, SUMOylation, and NEDDylation using purified ligase enzymes and extracts prepared from cultured cell lines and pathological specimens. We further validate this approach by confirming the in vivo modification of several novel PTM substrates identified by our assay. CONCLUSIONS/SIGNIFICANCE: This methodology offers several advantages over currently used PTM detection methods including ease of use, rapidity, scale, and sample source diversity. Furthermore, by allowing for the intrinsic enzymatic activities of cell populations or pathological states to be directly compared, this methodology could have widespread applications for the study of PTMs in human diseases and has the potential to be directly applied to most, if not all, basic PTM research.

  7. Profiling Physical Activity, Diet, Screen and Sleep Habits in Portuguese Children

    Directory of Open Access Journals (Sweden)

    Sara Pereira

    2015-06-01

    Full Text Available Obesity in children is partly due to unhealthy lifestyle behaviours, e.g., sedentary activity and poor dietary choices. This trend has been seen globally. To determine the extent of these behaviours in a Portuguese population of children, 686 children 9.5 to 10.5 years of age were studied. Our aims were to: (1 describe profiles of children’s lifestyle behaviours; (2 identify behaviour pattern classes; and (3 estimate combined effects of individual/ socio-demographic characteristics in predicting class membership. Physical activity and sleep time were estimated by 24-h accelerometry. Nutritional habits, screen time and socio-demographics were obtained. Latent Class Analysis was used to determine unhealthy lifestyle behaviours. Logistic regression analysis predicted class membership. About 78% of children had three or more unhealthy lifestyle behaviours, while 0.2% presented no risk. Two classes were identified: Class 1-Sedentary, poorer diet quality; and Class 2-Insufficiently active, better diet quality, 35% and 65% of the population, respectively. More mature children (Odds Ratio (OR = 6.75; 95%CI = 4.74–10.41, and boys (OR = 3.06; 95% CI = 1.98–4.72 were more likely to be overweight/obese. However, those belonging to Class 2 were less likely to be overweight/obese (OR = 0.60; 95% CI = 0.43–0.84. Maternal education level and household income did not significantly predict weight status (p ≥ 0.05.

  8. Characterization of inflammatory markers and transcriptome profiles of differentially activated embryonic stem cell-derived microglia.

    Science.gov (United States)

    Beins, Eva; Ulas, Thomas; Ternes, Svenja; Neumann, Harald; Schultze, Joachim L; Zimmer, Andreas

    2016-06-01

    Microglia, the immune cells of the CNS, are highly adaptive cells that can acquire different pro- and anti-inflammatory activation states with distinct functions in CNS homeostasis and pathologies. To study microglial function in vitro, primary microglia or immortalized cell lines are commonly used. An alternative to these cells are embryonic stem cell-derived microglia (ESdM). ESdM have previously been shown to be very similar to primary microglia in terms of expression profiles and surface molecules. In this study, ESdM and primary microglia were treated with different inflammatory stimulants to analyze their ability to adopt different activation states. Using quantitative real-time PCR, comparative transcriptomics, ELISA, and flow cytometry, we found that different activation states can be induced in ESdM, which are similar to those found in primary microglia. These states are characterized by specific sets of inflammatory marker molecules and differential transcriptome signatures. Our results show that ESdM are a valuable alternative cell model to study microglial functions and neuroinflammatory mechanisms.

  9. Oxidative Profile and δ-Aminolevulinate Dehydratase Activity in Healthy Pregnant Women with Iron Supplementation.

    Science.gov (United States)

    De Lucca, Leidiane; Rodrigues, Fabiane; Jantsch, Letícia B; Neme, Walter S; Gallarreta, Francisco M P; Gonçalves, Thissiane L

    2016-05-03

    An oxidative burst occurs during pregnancy due to the large consumption of oxygen in the tissues and an increase in metabolic demands in response to maternal physiological changes and fetal growth. This study aimed to determine the oxidative profile and activity of δ-aminolevulinate dehydratase (δ-ALA-D) in pregnant women who received iron supplementation. Oxidative stress parameters were evaluated in 25 pregnant women with iron supplementation, 25 pregnant women without supplementation and 25 non-pregnant women. The following oxidative stress parameters were evaluated: thiobarbituric acid reactive substances (TBARS), protein thiol groups (P-SH), non-protein thiol levels (NP-SH), vitamin C levels, catalase and δ-ALA-D activity. Markers of oxidative stress and cell damage, such as TBARS in plasma were significantly higher in pregnant women without supplementation. Levels of P-SH, NP-SH and δ-ALA-D activity were significantly lower in pregnant women without supplementation compared to non-pregnant and pregnant women with supplementation, while vitamin C levels were significantly lower in pregnant women without supplementation when compared to non-pregnant women. The increase in the generation of oxidative species and decrease of antioxidants suggest the loss of physiological oxidative balance during normal pregnancy, which was not observed in pregnant women with iron supplementation, suggesting a protective effect of iron against oxidative damage.

  10. Profiling physical activity, diet, screen and sleep habits in Portuguese children.

    Science.gov (United States)

    Pereira, Sara; Katzmarzyk, Peter T; Gomes, Thayse Natacha; Borges, Alessandra; Santos, Daniel; Souza, Michele; dos Santos, Fernanda K; Chaves, Raquel N; Champagne, Catherine M; Barreira, Tiago V; Maia, José A R

    2015-06-02

    Obesity in children is partly due to unhealthy lifestyle behaviours, e.g., sedentary activity and poor dietary choices. This trend has been seen globally. To determine the extent of these behaviours in a Portuguese population of children, 686 children 9.5 to 10.5 years of age were studied. Our aims were to: (1) describe profiles of children's lifestyle behaviours; (2) identify behaviour pattern classes; and (3) estimate combined effects of individual/ socio-demographic characteristics in predicting class membership. Physical activity and sleep time were estimated by 24-h accelerometry. Nutritional habits, screen time and socio-demographics were obtained. Latent Class Analysis was used to determine unhealthy lifestyle behaviours. Logistic regression analysis predicted class membership. About 78% of children had three or more unhealthy lifestyle behaviours, while 0.2% presented no risk. Two classes were identified: Class 1-Sedentary, poorer diet quality; and Class 2-Insufficiently active, better diet quality, 35% and 65% of the population, respectively. More mature children (Odds Ratio (OR) = 6.75; 95%CI = 4.74-10.41), and boys (OR = 3.06; 95% CI = 1.98-4.72) were more likely to be overweight/obese. However, those belonging to Class 2 were less likely to be overweight/obese (OR = 0.60; 95% CI = 0.43-0.84). Maternal education level and household income did not significantly predict weight status (p ≥ 0.05).

  11. Profiling Physical Activity, Diet, Screen and Sleep Habits in Portuguese Children

    Science.gov (United States)

    Pereira, Sara; Katzmarzyk, Peter T.; Gomes, Thayse Natacha; Borges, Alessandra; Santos, Daniel; Souza, Michele; dos Santos, Fernanda K.; Chaves, Raquel N.; Champagne, Catherine M.; Barreira, Tiago V.; Maia, José A.R.

    2015-01-01

    Obesity in children is partly due to unhealthy lifestyle behaviours, e.g., sedentary activity and poor dietary choices. This trend has been seen globally. To determine the extent of these behaviours in a Portuguese population of children, 686 children 9.5 to 10.5 years of age were studied. Our aims were to: (1) describe profiles of children’s lifestyle behaviours; (2) identify behaviour pattern classes; and (3) estimate combined effects of individual/socio-demographic characteristics in predicting class membership. Physical activity and sleep time were estimated by 24-h accelerometry. Nutritional habits, screen time and socio-demographics were obtained. Latent Class Analysis was used to determine unhealthy lifestyle behaviours. Logistic regression analysis predicted class membership. About 78% of children had three or more unhealthy lifestyle behaviours, while 0.2% presented no risk. Two classes were identified: Class 1-Sedentary, poorer diet quality; and Class 2-Insufficiently active, better diet quality, 35% and 65% of the population, respectively. More mature children (Odds Ratio (OR) = 6.75; 95%CI = 4.74–10.41), and boys (OR = 3.06; 95% CI = 1.98–4.72) were more likely to be overweight/obese. However, those belonging to Class 2 were less likely to be overweight/obese (OR = 0.60; 95% CI = 0.43–0.84). Maternal education level and household income did not significantly predict weight status (p ≥ 0.05). PMID:26043034

  12. On some exact solutions of slightly variant forms of Yang's equations and their graphical representations

    Indian Academy of Sciences (India)

    Rupesh Kumar Sha; Pranab Krishna Chanda

    2008-05-01

    The equations obtained by Yang while discussing the condition of self-duality of (2) gauge fields on Euclidean four-dimensional space have been generalized. Exact solutions and their graphical representations for the generalized equation (for some particular values of the parameters) have been reported. They represent interesting physical characteristics like waves with spreading solitary profile, spreading wave packets, waves with pulsating solitary profile (between zero and a maximum), waves with oscillatory solitary profile and chaos.

  13. Lipschitz Parametrization of Probabilistic Graphical Models

    CERN Document Server

    Honorio, Jean

    2012-01-01

    We show that the log-likelihood of several probabilistic graphical models is Lipschitz continuous with respect to the lp-norm of the parameters. We discuss several implications of Lipschitz parametrization. We present an upper bound of the Kullback-Leibler divergence that allows understanding methods that penalize the lp-norm of differences of parameters as the minimization of that upper bound. The expected log-likelihood is lower bounded by the negative lp-norm, which allows understanding the generalization ability of probabilistic models. The exponential of the negative lp-norm is involved in the lower bound of the Bayes error rate, which shows that it is reasonable to use parameters as features in algorithms that rely on metric spaces (e.g. classification, dimensionality reduction, clustering). Our results do not rely on specific algorithms for learning the structure or parameters. We show preliminary results for activity recognition and temporal segmentation.

  14. Distinct BOLD activation profiles following central and peripheral oxytocin administration in awake rats

    Directory of Open Access Journals (Sweden)

    Craig F Ferris

    2015-09-01

    Full Text Available A growing body of literature has suggested that intranasal oxytocin (OT or other systemic routes of administration can alter prosocial behavior, presumably by directly activating OT sensitive neural circuits in the brain. Yet there is no clear evidence that OT given peripherally can cross the blood-brain-barrier at levels sufficient to engage the OT receptor. To address this issue we examined changes in blood oxygen level dependent (BOLD signal intensity in response to peripheral OT injections (0.1, 0.5 or 2.5 mg/kg during functional magnetic resonance (fMRI in awake rats imaged at 7.0 tesla. These data were compared to OT (1ug/5 µl given directly to the brain via the lateral cerebroventricle. Using a 3D annotated MRI atlas of the rat brain segmented into 171 brain areas and computational analysis we reconstructed the distributed integrated neural circuits identified with BOLD fMRI following central and peripheral OT. Both routes of administration caused significant changes in BOLD signal within the first 10 min of administration. As expected, central OT activated a majority of brain areas known to express a high density of OT receptors e.g., lateral septum, subiculum, shell of the accumbens, bed nucleus of the stria terminalis. This profile of activation was not matched by peripheral OT. The change in BOLD signal to peripheral OT did not show any discernible dose-response. Interestingly, peripheral OT affected all subdivisions of the olfactory bulb, in addition to the cerebellum and several brainstem areas relevant to the autonomic nervous system, including the solitary tract nucleus. The results from this imaging study do not support a direct central action of peripheral OT on the brain. Instead, the patterns of brain activity suggest that peripheral OT may interact at the level of the olfactory bulb and through sensory afferents from the autonomic nervous system to influence brain activity.

  15. Behaviorally activated mRNA expression profiles produce signatures of learning and enhanced inhibition in aged rats with preserved memory.

    Directory of Open Access Journals (Sweden)

    Rebecca P Haberman

    Full Text Available Aging is often associated with cognitive decline, but many elderly individuals maintain a high level of function throughout life. Here we studied outbred rats, which also exhibit individual differences across a spectrum of outcomes that includes both preserved and impaired spatial memory. Previous work in this model identified the CA3 subfield of the hippocampus as a region critically affected by age and integral to differing cognitive outcomes. Earlier microarray profiling revealed distinct gene expression profiles in the CA3 region, under basal conditions, for aged rats with intact memory and those with impairment. Because prominent age-related deficits within the CA3 occur during neural encoding of new information, here we used microarray analysis to gain a broad perspective of the aged CA3 transcriptome under activated conditions. Behaviorally-induced CA3 expression profiles differentiated aged rats with intact memory from those with impaired memory. In the activated profile, we observed substantial numbers of genes (greater than 1000 exhibiting increased expression in aged unimpaired rats relative to aged impaired, including many involved in synaptic plasticity and memory mechanisms. This unimpaired aged profile also overlapped significantly with a learning induced gene profile previously acquired in young adults. Alongside the increased transcripts common to both young learning and aged rats with preserved memory, many transcripts behaviorally-activated in the current study had previously been identified as repressed in the aged unimpaired phenotype in basal expression. A further distinct feature of the activated profile of aged rats with intact memory is the increased expression of an ensemble of genes involved in inhibitory synapse function, which could control the phenotype of neural hyperexcitability found in the CA3 region of aged impaired rats. These data support the conclusion that aged subjects with preserved memory recruit

  16. GRADE: a graphical programming environment for multicomputers

    OpenAIRE

    Kacsuk, P; G. Dózsa; T. Fadfyas; R. Lovas

    2012-01-01

    To provide high-level graphical support for developing message passing programs, an integrated programming environment (GRADE) is being developed. GRADE currently provides tools to construct, execute, debug, monitor and visualize message passing based parallel programs. GRADE offers the programmer an integrated graphical user interface during the whole life-cycle of program development and provides high-level graphical programming abstraction mechanisms to construct parallel applications. The...

  17. 创意思维与图形设计%Creative Thinking and Graphic Design

    Institute of Scientific and Technical Information of China (English)

    葛饶民

    2004-01-01

    In commercial activities, graphic design needs to be effective in its visual language in order to accurately communicate its message to consumers. To achieve this, designers need creative thinking, among which diffusible thinking and retrorse thinking are of the first importance. This article analyzes the various factors and methods needed to be considered during the creative graphic design.

  18. Using Interactive Graphics to Teach Multivariate Data Analysis to Psychology Students

    Science.gov (United States)

    Valero-Mora, Pedro M.; Ledesma, Ruben D.

    2011-01-01

    This paper discusses the use of interactive graphics to teach multivariate data analysis to Psychology students. Three techniques are explored through separate activities: parallel coordinates/boxplots; principal components/exploratory factor analysis; and cluster analysis. With interactive graphics, students may perform important parts of the…

  19. Guidebook to R graphics using Microsoft Windows

    CERN Document Server

    Takezawa, Kunio

    2012-01-01

    Introduces the graphical capabilities of R to readers new to the software Due to its flexibility and availability, R has become the computing software of choice for statistical computing and generating graphics across various fields of research. Guidebook to R Graphics Using Microsoft® Windows offers a unique presentation of R, guiding new users through its many benefits, including the creation of high-quality graphics. Beginning with getting the program up and running, this book takes readers step by step through the process of creating histograms, boxplots, strip charts, time series gra

  20. Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry (Gaultheria procumbens L. Leaf Extracts

    Directory of Open Access Journals (Sweden)

    Piotr Michel

    2014-12-01

    Full Text Available Dry leaf extracts of eastern teaberry (Gaultheria procumbens L. were evaluated as a source of bioactive phytocompounds through systematic activity testing and phytochemical profiling. The antioxidant efficiency was tested using five complementary in vitro models (DPPH; FRAP; linoleic acid (LA peroxidation assay; O2•− and H2O2 scavenging tests in parallel with standard antioxidants. The 75% methanol extract and its diethyl ether, ethyl acetate (EAF, n-butanol and water fractions exhibited the dose-dependent responses in all assays, with the highest capacities found for EAF (DPPH EC50 = 2.9 μg/mL; FRAP = 12.8 mmol Fe2+/g; IC50 for LA-peroxidation = 123.9 μg/mL; O2•− SC50 = 3.9 μg/mL; H2O2 SC50 = 7.2 μg/mL. The EAF had also the highest anti-inflammatory activity in the inhibition tests of lipoxygenase and hyaluronidase (60.14% and 21.83% effects, respectively, at the concentration of 100 μg/mL. Activity parameters of the extracts correlated strongly with the levels of total phenolics (72.4–270.7 mg GAE/g, procyanidins, and phenolic acids, whereas for flavonoids only moderate effects were observed. Comprehensive UHPLC-PDA-ESI-MS3 and HPLC-PDA studies led to the identification of 35 polyphenols with a procyanidin A-type trimer, quercetin 3-O-glucuronide, isomers of caffeoylquinic acids, and (‒-epicatechin being the dominant components. Significant activity levels, high phenolic contents and high extraction yields (39.4%–42.5% DW for defatted and crude methanol extracts, respectively indicate the value of eastern teaberry leaves as bioactive products.

  1. Comparison of Activity Profiles and Physiological Demands Between International Rugby Sevens Matches and Training.

    Science.gov (United States)

    Higham, Dean G; Pyne, David B; Anson, Judith M; Hopkins, Will G; Eddy, Anthony

    2016-05-01

    The specificity of contemporary training practices of international rugby sevens players is unknown. We quantified the positional group-specific activity profiles and physiological demands of on-field training activities and compared these with match demands. Twenty-two international matches and 63 rugby-specific training drills were monitored in 25 backs and 17 forwards from a national squad of male rugby sevens players over a 21-month period. Drills were classified into 3 categories: low-intensity skill refining (n = 23 drills, 560 observations), moderate- to high-intensity skill refining (n = 28 drills, 600 observations), and game simulation (n = 12 drills, 365 observations). Movement patterns (via Global Positioning System devices) and physiological load (via heart rate monitors) were recorded for all activities, and the differences between training and matches were quantified using magnitude-based inferential statistics. Distance covered in total and at ≥3.5 m·s, maximal velocity, and frequency of accelerations and decelerations were lower for forwards during competition compared with those for backs by a small but practically important magnitude. No clear positional group differences were observed for physiological load during matches. Training demands exceeded match demands only for frequency of decelerations of forwards during moderate- to high-intensity skill-refining drills and only by a small amount. Accelerations and distance covered at ≥6 m·s were closer to match values for forwards than for backs during all training activities, but training drills consistently fell below the demands of international competition. Coaches could therefore improve physical and physiological specificity by increasing the movement demands and intensity of training drills.

  2. Phytochemical profiles and antioxidant activities in six species of ramie leaves.

    Directory of Open Access Journals (Sweden)

    Yongsheng Chen

    Full Text Available Increased consumption of vegetables or plant food has been associated with decreased risk of developing major chronic diseases, such as cancers, diabetes, cardiovascular diseases, and age-related functional decline. Ramie leaves are rich in phenolics and flavonoids, which have been suggested for human health benefits. Phenolic contents, flavonoid contents, phenolic compounds, and anti-cancer properties in six species of ramie leaves were analyzed by Folin-reagent method, sodium borohydride/chloranil-based assay (SBC, HPLC method and antiproliferation, cytoxicity, respectively. Antioxidant activities were measured through peroxyl radical scavenging capacity (PSC method, oxygen radical absorbance capacity (ORAC method, and cellular antioxidant activity (CAA. Research indicated that Boehmeria penduliflora contained the highest total phenolic content (2313.7±27.28 mg GAE/100 g FW, and flavonoid content (1682.4±27.70 mg CAE/100 g FW. Boehmeria tricuspis showed the highest PSC value (9574.8±117.63 µM vit. C equiv./100 g FW, while Boehmeria penduliflora indicated the highest ORAC value (330.44±16.88 µmol Trolox equiv./g FW. The antioxidant activities were correlated with phenolic contents and flavonoid contents. Boehmeria tricuspis had the highest antiproliferative capacity with the lowest EC₅₀ (4.11±0.19 mg/mL. The results for the analyzed ramie for CAA were significantly different from each other (p<0.05, Boehmeria tricuspis had the highest CAA value (133.63±7.10 µmol QE/100 g. Benzoic acid, 4-coumaric acid, caffeic acid, and ferulic acid were the dominant phenolic ingredients in the ramie leaves according to HPLC analysis. Our research is the first report to study the phytochemical profiles and antioxidant activities in different species of ramie leaves for their health benefit.

  3. Liquid chromatography-bioassay-mass spectrometry for profiling of physiologically active food.

    Science.gov (United States)

    Morlock, Gertrud E; Klingelhöfer, Ines

    2014-08-19

    Complex samples like food contain thousands of single compounds. In the past, only known target compounds were looked for; however, most bioactive compounds in food are unknown. On the contrary, nontarget analyses face the challenge of determining the thousand peaks' identities, but it remains largely unclear which peaks are bioactive. Here, we show a novel effect-directed food profiling, as food and food supplements can be unknowingly physiologically active. By the combination of planar chromatography, using water-wettable reversed phase high-performance thin-layer chromatography (HPTLC RP18 W) plates, with detection by specific microorganisms, endocrine compounds in food were quantitatively detected as sharp-bounded zones and further characterized by mass spectrometry. This analytical workflow allowed frequent food intakes to be identified as risky with regard to estrogen-effective compounds, in discussion for their potential involvement in foodborne pathogenesis and for use in personalized health care. Using this accelerated workflow with its comprehensive detection potential, unknown endocrine compounds can be discovered. Exemplarily, the discovery of up to six endocrine disrupting compounds was shown in seven propolis samples and in four spices. For example, microorganisms quantitatively detected an estrogen-effective compound in the range of 0.07-0.24% in seven propolis samples, which was assigned to be caffeic acid phenethyl ester by mass spectrometry. This streamlined nontarget analysis detected modes of action, followed by targeted characterization of newly discovered effective compounds. Also, drug discovery or analysis of traditional medicines may profit from this effect-directed profiling of complex samples.

  4. Multitaxon activity profiling reveals differential microbial response to reduced seawater pH and oil pollution.

    Science.gov (United States)

    Coelho, Francisco J R C; Cleary, Daniel F R; Costa, Rodrigo; Ferreira, Marina; Polónia, Ana R M; Silva, Artur M S; Simões, Mário M Q; Oliveira, Vanessa; Gomes, Newton C M

    2016-09-01

    There is growing concern that predicted changes to global ocean chemistry will interact with anthropogenic pollution to significantly alter marine microbial composition and function. However, knowledge of the compounding effects of climate change stressors and anthropogenic pollution is limited. Here, we used 16S and 18S rRNA (cDNA)-based activity profiling to investigate the differential responses of selected microbial taxa to ocean acidification and oil hydrocarbon contamination under controlled laboratory conditions. Our results revealed that a lower relative abundance of sulphate-reducing bacteria (Desulfosarcina/Desulfococcus clade) due to an adverse effect of seawater acidification and oil hydrocarbon contamination (reduced pH-oil treatment) may be coupled to changes in sediment archaeal communities. In particular, we observed a pronounced compositional shift and marked reduction in the prevalence of otherwise abundant operational taxonomic units (OTUs) belonging to the archaeal Marine Benthic Group B and Marine Hydrothermal Vent Group (MHVG) in the reduced pH-oil treatment. Conversely, the abundance of several putative hydrocarbonoclastic fungal OTUs was higher in the reduced pH-oil treatment. Sediment hydrocarbon profiling, furthermore, revealed higher concentrations of several alkanes in the reduced pH-oil treatment, corroborating the functional implications of the structural changes to microbial community composition. Collectively, our results advance the understanding of the response of a complex microbial community to the interaction between reduced pH and anthropogenic pollution. In future acidified marine environments, oil hydrocarbon contamination may alter the typical mixotrophic and k-/r-strategist composition of surface sediment microbiomes towards a more heterotrophic state with lower doubling rates, thereby impairing the ability of the ecosystem to recover from acute oil contamination events.

  5. Chromatogram Profiles and Cytotoxic Activity of Irradiated Mahkota Dewa (Phaleria Macrocarpa Scheff. Boerl Leaves

    Directory of Open Access Journals (Sweden)

    E. Katrin1

    2011-04-01

    Full Text Available Gamma irradiation has been used by the industries for preservation of herbal medicine, but it has not been studied the effect of gamma irradiation on their efficacy, especially their bioactivity as anticancer substances. The purpose of this research was to study the effect of gamma irradiation on the mahkota dewa leaves which has been claimed to contain potent anticancer substances. Maceration of dried mahkota dewa leaves successively with n-hexane, ethyl acetate, and ethanol gave crude extracts which the ethyl acetate was the most cytotoxic extract against leukemia L1210 cells with an inhibition concentration fifty (IC50 value of 10.3 µg/ml. Further separation of ethyl acetate extract by column chromatograph gave 7 fractions, and fraction 2 showed the most cytotoxic fraction exhibited the most cytotoxic extract against leukemia L1210 cells with an IC50 value of 1.9 µg/ml. Since, the fraction 2 of ethyl acetate extract was the most potent fraction, the irradiated samples were treated with the same procedure as treatment of fraction 2 from control sample. Cytotoxic activity test of fractions 2 from irradiated samples showed that the cytotoxic activity decreased depending on increasing of irradiation dose. Gamma irradiation dose up to 7.5 kGy on mahkota dewa leaves could decreased the cytotoxic activity of fraction 2 as the most cytotoxic-potential fraction against leukemia L1210 cells, but decreasing the cytotoxic activity has not exceeded the limit of the fraction declared inactive. So that the irradiation dose up to 7.5 kGy can be used for decontamination of bacteria and fungus/yeast without eliminating the cytotoxic activity. Gamma irradiation also caused changes in the thin layer chromatograph (TLC spots and HPLC chromatograms profiles of fraction 2 which was the most cytotoxic fraction in ethyl acetate extract of mahkota dewa leaves against leukemia L1210 cells. One of the main peaks (peak 1 on HPLC chromatograms decreased with increasing

  6. Insights on the Phytochemical Profile (Cyclopeptides and Biological Activities of Calotropis procera Latex Organic Fractions

    Directory of Open Access Journals (Sweden)

    Thiago Lustosa Jucá

    2013-01-01

    Full Text Available Calotropis procera is a medicinal plant whose pharmacological properties are associated with its latex. Here, the Calotropis procera latex fractions were investigated in an attempt to trace its phytochemical profile and measure its anti-inflammatory and toxicity activity. The crude latex was partitioned, yielding five fractions (49.4% hexane, 5.2% dichloromethane, 2.0% ethyl acetate, 2.1% n-butanol, and 41.1% aqueous. Phytochemical screening and spectroscopy analysis revealed that dichloromethane is the most chemically diverse fraction. Triterpenes were detected in both the hexane and dichloromethane fractions, while flavonoids were detected in the dichloromethane and ethyl acetate fractions. These fractions were cytotoxic to cancer cell lines (LD50 0.05 to 3.9 μg/mL and lethal to brine shrimp (LD50 10.9 to 65.7 μg/mL. Reduced neutrophil migration in rats was observed in carrageenan-induced peritonitis for the dichloromethane (67%, ethyl acetate (56%, and aqueous (72% fractions. A positive reaction with tolidine and ninhydrin suggested that cyclopeptides are in the ethyl acetate fraction. It is therefore concluded that Calotropis procera latex dichloromethane and ethyl acetate fractions exhibit both in vitro and in vivo activities as well as anti-inflammatory properties. Cyclopeptide detection is especially interesting because previous attempts to investigate these low-molecular cyclic amino acid sequences in C. procera have failed.

  7. Enhancing the T-shaped learning profile when teaching hydrology using data, modeling, and visualization activities

    Science.gov (United States)

    Sanchez, Christopher A.; Ruddell, Benjamin L.; Schiesser, Roy; Merwade, Venkatesh

    2016-03-01

    Previous research has suggested that the use of more authentic learning activities can produce more robust and durable knowledge gains. This is consistent with calls within civil engineering education, specifically hydrology, that suggest that curricula should more often include professional perspective and data analysis skills to better develop the "T-shaped" knowledge profile of a professional hydrologist (i.e., professional breadth combined with technical depth). It was expected that the inclusion of a data-driven simulation lab exercise that was contextualized within a real-world situation and more consistent with the job duties of a professional in the field, would provide enhanced learning and appreciation of job duties beyond more conventional paper-and-pencil exercises in a lower-division undergraduate course. Results indicate that while students learned in both conditions, learning was enhanced for the data-driven simulation group in nearly every content area. This pattern of results suggests that the use of data-driven modeling and visualization activities can have a significant positive impact on instruction. This increase in learning likely facilitates the development of student perspective and conceptual mastery, enabling students to make better choices about their studies, while also better preparing them for work as a professional in the field.

  8. HPLC-TOF/MS profile and nitric oxide scavenging activity of Orthosiphon stamineus leaf extracts

    Institute of Scientific and Technical Information of China (English)

    Gabriel Akyirem Akowuah; Zhari Ismail; Maraiam Ahmad

    2012-01-01

    Objective: The aim of the present study is to develop liquid chromatography (LC)/Time-of-flight mass spectrometry (TOF/MS) profile for methanol and water extracts of Orthosiphon stamineus leaf using SEN and RA as flavonoid and non-flavonoid polyphenolic markers in the extracts. The study also evaluates in vitro nitric oxide radical scavenging effect of the extracts. Method:Orthogonal Time of Flight Mass Spectrometer equipped with HPLC separation module was used in the analyses of the extract. The in vitro nitric oxide scavenging activity of the extracts was measured according to the method described by Rao. Results: The qualitative analysis of the extracts performed with HPLC-TOF/MS confirmed the presence sinensitin (SEN) and rosmarinic acid (RA) in the extracts. The extracts showed in vitro nitric oxide scavenging activities. Conclusions: The HPLC-TOF/MS method could be employed for quality determination of herbal medicinal products and formulations containing O. stamineus. The extracts may play a significant role in prevention of degenerate disease due to its ability to scavenge nitric oxide radical.

  9. Biodiversity, Anti-Trypanosomal Activity Screening, and Metabolomic Profiling of Actinomycetes Isolated from Mediterranean Sponges.

    Directory of Open Access Journals (Sweden)

    Cheng Cheng

    Full Text Available Marine sponge-associated actinomycetes are considered as promising sources for the discovery of novel biologically active compounds. In the present study, a total of 64 actinomycetes were isolated from 12 different marine sponge species that had been collected offshore the islands of Milos and Crete, Greece, eastern Mediterranean. The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. Four putatively novel species belonging to genera Geodermatophilus, Microlunatus, Rhodococcus and Actinomycetospora were identified based on a 16S rRNA gene sequence similarity of < 98.5% to currently described strains. Eight actinomycete isolates showed bioactivities against Trypanosma brucei brucei TC221 with half maximal inhibitory concentration (IC50 values <20 μg/mL. Thirty four isolates from the Milos collection and 12 isolates from the Crete collection were subjected to metabolomic analysis using high resolution LC-MS and NMR for dereplication purposes. Two isolates belonging to the genera Streptomyces (SBT348 and Micromonospora (SBT687 were prioritized based on their distinct chemistry profiles as well as their anti-trypanosomal activities. These findings demonstrated the feasibility and efficacy of utilizing metabolomics tools to prioritize chemically unique strains from microorganism collections and further highlight sponges as rich source for novel and bioactive actinomycetes.

  10. Antiradical activity, phenolics profile, and hydroxymethylfurfural in espresso coffee: influence of technological factors.

    Science.gov (United States)

    Alves, Rita C; Costa, Anabela S G; Jerez, María; Casal, Susana; Sineiro, Jorge; Núñez, María J; Oliveira, Beatriz

    2010-12-01

    The influence of technological factors (decaffeination, brew volume, coffee species, and roast degree) on antiradical activity and phenolics content of espresso coffee is described. The screenings of phenolics profile and other compounds (caffeine and trigonelline), as well as the quantification of hydroxymethylfurfural, were performed by LC-DAD-ESI-MS. Significantly lower (p decaffeinated espressos when compared with regular ones (32 vs 38% and 324 vs 410 mg/30 mL cup, respectively). A long espresso (70 mL) offers more than twice the phenolics amount of a short one (20 mL). Robusta brews showed higher (p 0.05) were observed for scavenging activities of differently roasted robusta brews, whereas an increase in medium-dark brews was observed for arabica samples. Total phenolics in robusta espressos decreased (p 0.05) were found between arabica espressos from different roasts. By LC-DAD-ESI-MS, 23 hydroxycinnamic derivatives were found, including chlorogenic acids, lactones, and cinnamoyl-amino acid conjugates. The amount of each compound was differently affected by species and roast. Robusta brews presented superior levels of caffeine and chlorogenic acids, whereas arabica ones contained more trigonelline. Hydroxymethylfurfural contents in the brew (30 mL) varied from 2.60 to 0.84 mg for light- and dark-roasted arabicas and from 1.29 to 0.68 mg for light- and dark-roasted robustas, respectively.

  11. A prospective study of injury and activity profile in elite soccer referees and assistant referees.

    LENUS (Irish Health Repository)

    Wilson, F

    2006-01-01

    Injuries to soccer players have been extensively examined, but not the injury experience of referees and assistant referees. This study aimed to determine the injury incidence and activity profile of soccer match officials. A 12 month prospective cohort study was used to collect activity and injury data of 31 participants who reported their training and match exposure and their injury incidence by means of weekly online questionnaire. Study participants spent a mean of 2632 hrs training and 1704 hrs officiating over the 12 month study period. Thirty eight injuries were recorded, (8.8 injuries\\/1000 hr of training (CI 6.2 to 12.0) and 16.4 injuries\\/1000 hr for match officiating (CI 10.9 to 23.8)), (Risk Ratio 4.3, 2.1 to 8.9). Fifty five percent (CI 40 to 70%) of the injuries were to muscles, and 76% (CI 61 to 87%) were to the lower leg. Overuse injuries represented 61% (CI 45 to 74%) of all cases. Findings showed that the injury frequency rate associated with soccer referees is higher than that in a number of other non contact sports. The injury incidence associated with training for soccer referees is higher than that associated with training for soccer players. Further prospective studies are merited to examine effectiveness and availability of injury management programmes to establish the welfare of this population.

  12. Isotope microscopy visualization of the adsorption profile of 2-methylisoborneol and geosmin in powdered activated carbon.

    Science.gov (United States)

    Matsui, Yoshihiko; Sakamoto, Asuka; Nakao, Soichi; Taniguchi, Takuma; Matsushita, Taku; Shirasaki, Nobutaka; Sakamoto, Naoya; Yurimoto, Hisayoshi

    2014-09-16

    Decreasing the particle size of powdered activated carbon may enhance its equilibrium adsorption capacity for small molecules and micropollutants, such as 2-methylisoborneol (MIB) and geosmin, as well as for macromolecules and natural organic matter. Shell adsorption, in which adsorbates do not completely penetrate the adsorbent but instead preferentially adsorb near the outer surface of the adsorbent, may explain this enhancement in equilibrium adsorption capacity. Here, we used isotope microscopy and deuterium-doped MIB and geosmin to directly visualize the solid-phase adsorbate concentration profiles of MIB and geosmin in carbon particles. The deuterium/hydrogen ratio, which we used as an index of the solid-phase concentration of MIB and geosmin, was higher in the shell region than in the inner region of carbon particles. Solid-phase concentrations of MIB and geosmin obtained from the deuterium/hydrogen ratio roughly agreed with those predicted by shell adsorption model analyses of isotherm data. The direct visualization of the localization of micropollutant adsorbates in activated carbon particles provided direct evidence of shell adsorption.

  13. Power output in vertical jumps: Does optimum loading depend on activity profiles?

    Science.gov (United States)

    Pazin, Nemanja; Berjan, Bobana; Nedeljkovic, Aleksandar; Markovic, Goran; Jaric, Slobodan

    2013-01-01

    The previously proposed Maximum Dynamic Output hypothesis (MDO; i.e. the optimum load for maximizing the power output during jumping is one's own body) was tested on individuals of various activity profiles. Forty males (10 strength-trained athletes, 10 speed-trained athletes, 10 physically active non-athletes, and 10 sedentary individuals) performed different vertical jumps on a force plate while a pulley system was used to either reduce or increase the subject's body weight by 10–30%. As expected, an increase in external loading resulted in a significant increase (p < 0.001) in force output and a concomitant decrease of peak jumping velocity in all groups of participants. The main finding, however, was that all groups revealed the maximum peak and mean power output at approximately the subjects’ own body weight although their weight represented prominently different percentage of their maximum dynamic strength. While a significant (p < 0.05), albeit moderate, 'group × load' interaction in one jump was observed for the peak power output, the individual optimum load for maximizing the power output number did not differ among the groups. Although apparently further research on various types of movements is needed, the present results provide, so far, the strongest support of the MDO hypothesis. PMID:22864398

  14. Graphic design as urban design: towards a theory for analysing graphic objects in urban environments.

    OpenAIRE

    2011-01-01

    This thesis presents a model for analysing the graphic object as urban object, by considering atypical fields of discourse that contribute to the formation of the object domain. The question: what is graphic design as urban design? directs the research through an epistemological design study comprising: an interrogation of graphic design studio practice and the articulation of graphic design research questions; a review and subsequent development of research strategy, design and method toward...

  15. THE USING OF GRAPHICAL EDITOR IN THE ENGINEERING GRAPHICS AND THE COURSE DESIGNING

    Directory of Open Access Journals (Sweden)

    KARPYUK L. V.

    2016-08-01

    Full Text Available The problems of learning students of the engineering and computer graphics of the course on the base of computer-aided design (CAD were described in the article. The examples of training tasks for acquiring knowledge of work in the environment of graphical editor of AutoCAD were shown. These examples are needed to perform drawings on The Engineering Graphics, and also for a graphic part of Course Projects for students of mechanical specialties.

  16. Gene expression profiling of pituitary melanotrope cells during their physiological activation.

    Science.gov (United States)

    Kuribara, Miyuki; van Bakel, Nick H M; Ramekers, Dyan; de Gouw, Daan; Neijts, Roel; Roubos, Eric W; Scheenen, Wim J J M; Martens, Gerard J M; Jenks, Bruce G

    2012-01-01

    The pituitary melanotrope cells of the amphibian Xenopus laevis are responsible for the production of the pigment-dispersing peptide α-melanophore-stimulating hormone, which allows the animal to adapt its skin color to its environment. During adaptation to a dark background the melanotrope cells undergo remarkable changes characterized by dramatic increases in cell size and secretory activity. In this study we performed microarray mRNA expression profiling to identify genes important to melanotrope activation and growth. We show a strong increase in the expression of the immediate early gene (IEG) c-Fos and of the brain-derived neurotrophic factor gene (BDNF). Furthermore, we demonstrate the involvement of another IEG in the adaptation process, Nur77, and conclude from in vitro experiments that the expression of both c-Fos and Nur77 are partially regulated by the adenylyl cyclase system and calcium ions. In addition, we found a steady up-regulation of Ras-like product during the adaptation process, possibly evoked by BDNF/TrkB signaling. Finally, the gene encoding the 105-kDa heat shock protein HSPh1 was transiently up-regulated in the course of black-background adaptation and a gene product homologous to ferritin (ferritin-like product) was >100-fold up-regulated in fully black-adapted animals. We suggest that these latter two genes are induced in response to cellular stress and that they may be involved in changing the mode of mRNA translation required to meet the increased demand for de novo protein synthesis. Together, our results show that microarray analysis is a valuable approach to identify the genes responsible for generating coordinated responses in physiologically activated cells.

  17. Spatial Ability through Engineering Graphics Education

    Science.gov (United States)

    Marunic, Gordana; Glazar, Vladimir

    2013-01-01

    Spatial ability has been confirmed to be of particular importance for successful engineering graphics education and to be a component of human intelligence that can be improved through instruction and training. Consequently, the creation and communication by means of graphics demand careful development of spatial skills provided by the balanced…

  18. Computer Graphics in ChE Education.

    Science.gov (United States)

    Reklaitis, G. V.; And Others

    1983-01-01

    Examines current uses and future possibilities of computer graphics in chemical engineering, discussing equipment needs, maintenance/manpower costs, and plan to implement computer graphics into existing programs. The plan involves matching fund equipment grants, grants for development of computer assisted instructional (CAI) software, chemical…

  19. Getting Graphic at the School Library.

    Science.gov (United States)

    Kan, Kat

    2003-01-01

    Provides information for school libraries interested in acquiring graphic novels. Discusses theft prevention; processing and cataloging; maintaining the collection; what to choose, with two Web sites for more information on graphic novels for libraries; collection development decisions; and Japanese comics called Manga. Includes an annotated list…

  20. Graphical modelling software in R - status

    DEFF Research Database (Denmark)

    Dethlefsen, Claus; Højsgaard, Søren; Lauritzen, Steffen L.

    Graphical models in their modern form have been around for nearly a quarter of a century.  Various computer programs for inference in graphical models have been developed over that period. Some examples of free software programs are BUGS (Thomas 1994), CoCo (Badsberg2001), Digram (Klein, Keiding...

  1. Introduction to Graphics Programming in Java

    DEFF Research Database (Denmark)

    Rosendahl, Mads

    Writing graphics applications in Java using Swing can be quite a daunting experience which requires understanding of some large libraries, and fairly advanced aspects of Java. In these notes we will show that by using a small subset of the Swing package we can write a write range of graphics...

  2. Performing Remarkable Feats with Presentation Graphics Packages.

    Science.gov (United States)

    Ekhaml, Leticia

    1994-01-01

    Describes easy-to-use, easy-to-learn dedicated graphics packages for developing slide presentations shown directly from computers. A general description of the features of five packages and suggestions for software selection are provided. A sidebar provides graphics tips, such as the use of color, typefaces, and formatting, for developing…

  3. Mastering probabilistic graphical models using Python

    CERN Document Server

    Ankan, Ankur

    2015-01-01

    If you are a researcher or a machine learning enthusiast, or are working in the data science field and have a basic idea of Bayesian learning or probabilistic graphical models, this book will help you to understand the details of graphical models and use them in your data science problems.

  4. Teaching Media Literacy with Graphic Novels

    Science.gov (United States)

    Monnin, Katie

    2010-01-01

    Background: A current problem in media literacy studies is whether or not to categorize graphic novels as media literacy texts. Thus, this article begins with a review of current media literacy research and its emphasis on defining media literacy texts as texts that rely on both print literacies and image literacies. Because graphic novels rely on…

  5. Exploring Literary Devices in Graphic Novels

    Science.gov (United States)

    Dallacqua, Ashley K.

    2012-01-01

    This article explores the possibilities of graphic novels with young readers. During the 2009-2010 school year, while working with four fifth-grade students, the author examined the question "In what ways do readers engage while reading a graphic novel?" The fifth graders took part in book discussions and one-on-one interviews after reading two…

  6. Graphical Models and Computerized Adaptive Testing.

    Science.gov (United States)

    Mislevy, Robert J.; Almond, Russell G.

    This paper synthesizes ideas from the fields of graphical modeling and education testing, particularly item response theory (IRT) applied to computerized adaptive testing (CAT). Graphical modeling can offer IRT a language for describing multifaceted skills and knowledge, and disentangling evidence from complex performances. IRT-CAT can offer…

  7. Graphic Organizers: Outlets for Your Thoughts.

    Science.gov (United States)

    Ekhaml, Leticia

    1998-01-01

    Graphs, bars, charts, and diagrams have been used by designers, writers, and scientists to communicate. Now, research suggests that graphic organizers benefit teaching and learning. This article describes graphic organizers: sequential, conceptual, hierarchical, cyclical, Venn, fishbone or Ishikawa, squeeze and stretch, why-why, t-chart, KWL…

  8. Standards of Multimedia Graphic Design in Education

    Science.gov (United States)

    Aldalalah, Osamah Ahmad; Ababneh, Ziad Waleed Mohamed

    2015-01-01

    This study aims to determine Standards of Multimedia Graphic Design in Education through the analysis of the theoretical basis and previous studies related to this subject. This study has identified the list of standards of Multimedia, Graphic Design, each of which has a set indicator through which the quality of Multimedia can be evaluated in…

  9. Graphic Design in Libraries: A Conceptual Process

    Science.gov (United States)

    Ruiz, Miguel

    2014-01-01

    Providing successful library services requires efficient and effective communication with users; therefore, it is important that content creators who develop visual materials understand key components of design and, specifically, develop a holistic graphic design process. Graphic design, as a form of visual communication, is the process of…

  10. Using Typography Grid in Graphic Design

    Institute of Scientific and Technical Information of China (English)

    张悦霞

    2007-01-01

    Typography grid is one of the important tools in the process of graphic design. Thought studying the history and principles of typography grid, we can know and master the methods of design and improve understanding of the aesthetics rules of graphic design.

  11. Q-Technique and Graphics Research.

    Science.gov (United States)

    Kahle, Roger R.

    Because Q-technique is as appropriate for use with visual and design items as for use with words, it is not stymied by the topics one is likely to encounter in graphics research. In particular Q-technique is suitable for studying the so-called "congeniality" of typography, for various copytesting usages, and for multivariate graphics research. The…

  12. Solar energy in Australia: a profile of renewable energy activity in its national context

    Energy Technology Data Exchange (ETDEWEB)

    Case, G.L.

    1980-08-01

    The following topics are included: country overview; energy summary; geopolitical, economic, and cultural aspects of Australia; the energy profile; and international agreements, contacts, manufacturers, and projects. (MHR)

  13. Activity Profiles and Physiological Responses of Representative Tag Football Players in Relation to Playing Position and Physical Fitness.

    Directory of Open Access Journals (Sweden)

    Luke W Hogarth

    Full Text Available This study determined the physical fitness, match-activity profiles and physiological responses of representative tag football players and examined the relationship between physical fitness and the match-activity profile. Microtechnology devices and heart rate (HR chest straps were used to determine the match-activity profiles of sixteen tag football players for five matches during the 2014 Australian National Championships. The relationships between lower body muscular power, straight line running speed and Yo-Yo intermittent recovery test level 2 (Yo-Yo IR2 and the match-activity profile were examined using Pearson's correlation coefficients. Outside players had greater lower body muscular power (ES = 0.98 and straight line running speed (ES = 1.03-1.18 than inside players, and also covered greater very high-speed running (VHSR distance/min (ES = 0.67 and reached higher peak running speeds (ES = 0.95 during matches. Inside and outside players performed a similar number of repeated high-intensity effort (RHIE bouts and reported similar mean and maximum efforts per RHIE bout. However, there were differences between playing positions for mean and maximal RHIE effort durations (ES = 0.69-1.15 and mean RHIE bout recovery (ES = 0.56. Inside and outside players also reported small to moderate differences (ES = 0.43-0.80 for times spent in each HR zone. There were a number of moderate to very large correlations between physical fitness measures and match-activity profile variables. This study found lower body muscular power, straight line running speed and Yo-Yo IR2 to be related to the match-activities of representative tag football players, although differences between inside and outside players suggest that athlete testing and training practices should be modified for different playing positions.

  14. Alkaloid profile, antibacterial and allelopathic activities of Lupinus jaimehintoniana B.L. Turner (Fabaceae

    Directory of Open Access Journals (Sweden)

    Ruiz-González Nancy

    2012-01-01

    Full Text Available Herein we describe some aspects of the ethnobotanical use and the first alkaloid profile of Lupinus jaimehintoniana, the 5 to 8 m high arboreous lupine. Five quinolizidine alkaloids identified as sparteine, 5,6-dehydrolupanine, lupanine, nuttalline, and d-thermopsine, were characterized by the respective elution order according to their electronic impact spectra, lupanine being the most abundant in the four different tissues analyzed. Simultaneously, an antibacterial assessment of the four corresponding crude methanolic extracts, as well as the four semi-purified alkaloids was performed on specific Escherichia coli and Agrobacterium tumefaciens strains. These experiments resulted in MIC ranges of 37-61 µg mL-1 and 130-146 µg mL-1, respectively. for both bacterial species. Finally, the allelopathic activity of these extracts on the germination of Lactuca sativa seeds was demonstrated to be in the range of 50-300 µg mL-1 for both semi-purified alkaloid and methanolic extracts.

  15. Chemical Profile, Antioxidant and Antibacterial Activities of Achillea moschata Wulfen, an Endemic Species from the Alps

    Directory of Open Access Journals (Sweden)

    Sara Vitalini

    2016-06-01

    Full Text Available Aerial parts of Achillea moschata Wulfen (Asteraceae growing wild in the Italian Rhaetian Alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. Inspection of the metabolic profile combining HPLC-DAD and ESI-MS/MS data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. Among them, the major compound was 7-O-glucosyl apigenin. Caffeoyl derivates were other phenolics identified. The essential oil obtained by steam distillation and investigated by GC/FID and GC/MS showed camphor, 1,8-cineole, and bornylacetate as the main constituents. The antioxidant capacity of three different extracts with increasing polarity and of the essential oil was evaluated by employing ABTS·+ and DPPH· radical scavenging assays. The methanolic extract was the only significantly effective sample against both synthetic radicals. All samples were also tested against Gram-positive (Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus and Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa bacterial species using the disk diffusion assay. The non-polar extracts (dichloromethane and petroleum ether and the essential oil possessed a broad spectrum of antimicrobial activity expressed according to inhibition zone diameter (8–24 mm.

  16. Dose profile modeling of Idaho National Laboratory's active neutron interrogation laboratory.

    Science.gov (United States)

    Chichester, D L; Seabury, E H; Zabriskie, J M; Wharton, J; Caffrey, A J

    2009-06-01

    A new laboratory has been commissioned at Idaho National Laboratory for performing active neutron interrogation research and development. The facility is designed to provide radiation shielding for deuterium-tritium (DT) fusion (14.1 MeV) neutron generators (2 x 10(8) n/s), deuterium-deuterium (DD) fusion (2.5 MeV) neutron generators (1 x 10(7) n/s), and (252)Cf spontaneous fission neutron sources (6.96 x 10(7) n/s, 30 microg). Shielding at the laboratory is comprised of modular concrete shield blocks 0.76 m thick with tongue-in-groove features to prevent radiation streaming, arranged into one small and one large test vault. The larger vault is designed to allow operation of the DT generator and has walls 3.8m tall, an entrance maze, and a fully integrated electrical interlock system; the smaller test vault is designed for (252)Cf and DD neutron sources and has walls 1.9 m tall and a simple entrance maze. Both analytical calculations and numerical simulations were used in the design process for the building to assess the performance of the shielding walls and to ensure external dose rates are within required facility limits. Dose rate contour plots have been generated for the facility to visualize the effectiveness of the shield walls and entrance mazes and to illustrate the spatial profile of the radiation dose field above the facility and the effects of skyshine around the vaults.

  17. Fatty Acid Profile and Biological Activities of Linseed and Rapeseed Oils

    Directory of Open Access Journals (Sweden)

    Anna Lewinska

    2015-12-01

    Full Text Available It has been postulated that fatty acids found in edible oils may exert beneficial health effects by the modulation of signaling pathways regulating cell differentiation and proliferation, especially in the treatment of cardiovascular diseases. In the present study, the biological effects of selected edible oils—linseed (LO and rapeseed (RO oils—were tested in vitro on fibroblast cells. The fatty acid profile of the oils was determined using gas chromatography and FTIR spectroscopy. LO was found to be rich in α-linolenic acid (ALA, whereas oleic acid was the most abundant species in RO. Fatty acids were taken up by the cells and promoted cell proliferation. No oxidative stress-mediated cytotoxic or genotoxic effects were observed after oil stimulation. Oils ameliorated the process of wound healing as judged by improved migration of fibroblasts to the wounding area. As ALA-rich LO exhibited the most potent wound healing activity, ALA may be considered a candidate for promoting the observed effect.

  18. Antioxidant activity, fatty acid profile and tocopherols of Tamarindus indica L. seeds

    Directory of Open Access Journals (Sweden)

    Débora Maria Moreno Luzia

    2011-06-01

    Full Text Available This study aimed to characterize Tamarindus indica L. seeds regarding its composition and to evaluate its antioxidant potential, fatty acid profile and content of tocopherols. In order to obtain the extract, the dried and crushed seeds were extracted with ethanol for 30 minutes in a 1:3 seeds: ethanol ratio under continuous stirring at room temperature. After that, the mixtures were filtered and subjected to roto-evaporation at 40 ºC in order to determine, through direct weighing, the dry matter yields of the extracts. According to the results, Tamarindus indica L. seeds showed high content of total carbohydrates (71.91% and offered relevant content and antioxidant activity of phenolic compounds. Tamarindus indica L. seeds oil presents high oxidative stability (15.83 hours and significant total tocopherol content (57.77 mg.kg-1, besides presenting a higher percentage of unsaturated fatty acids - the main component being linolenic (59.61%, which is considered an essential fatty acid.

  19. Influence of physical and emotional activity on the metabolic profile of blood serum of race horses

    Directory of Open Access Journals (Sweden)

    T. I. Bayeva

    2016-09-01

    Full Text Available In the article data are presented on dynamics of the level of indicators of metabolic profile of blood serum of race horses of the Ukrainian riding breed in the conditions of physical and emotional loading. Clinically healthy race horses were the object of  research. Blood was taken from the jugular vein to obtain serum and for further biochemical research. For the research 12 race horses from a training group were chosen. From time to time the animals took part in competitions; they were not specially used in races and were mostly used for the training of junior riders and sportsmen of different levels. Blood was taken in conditions of relative rest after ordinary training and after emotional stress during the entertainment performances when a large number of people were present and loud music was played. In the blood serum the following biochemical indicators were defined: whole protein, urea, creatinine, uric acid, total bilirubin and its fractions, glucose, cholestererol, triacylglycerol, calcium, ferrum, lactate, pyruvate, activity of the AlAT, SGOT, GGTP, LDH, an alkaline phosphatase – which makes it possible to determine reasonably accurately the adaptation potential of a horse under various types of loading. We established that during training and psychoemotional loading of racing horses of the training group of the Ukrainian riding breed, multidirectional changes in the level of biochemical indicators of blood serum occurred, which is evidence of stress in the metabolic processes in the animals’ organisms. Concentration of a biomarker of an oxidative stress, uric acid, increased after physical loading by 8.6%, and after emotional loading by 55.1%, which demonstrates that emotional stress had the more negative effect, indicating insufficient adaptation by the horses before demonstration performances. After physical loading, reaction of transamination in the horses’ liver cells intensified, and after emotional loading its intensity

  20. Changes in Coronary Heart Disease Risk Profile of Adults with Intellectual Disabilities following a Physical Activity Intervention

    Science.gov (United States)

    Moss, S. J.

    2009-01-01

    Background: Regular physical activity is one of the modifiable risk factors for coronary heart disease (CHD). With an increasing age profile and similar patterns of morbidity to the general population, persons with intellectual disabilities (ID) and their caregivers would benefit from data that indicate CHD risk factors. Knowledge of the CHD risk…

  1. Childhood-onset systemic lupus erythematosus in Singapore: clinical phenotypes, disease activity, damage, and autoantibody profiles.

    Science.gov (United States)

    Tan, J H T; Hoh, S F; Win, M T M; Chan, Y H; Das, L; Arkachaisri, T

    2015-08-01

    Childhood-onset systemic lupus erythematosus (cSLE) is a multisystem autoimmune disease characterized by immune dysregulation affecting patients less than 18 years old. One-fifth of SLE cases are diagnosed during childhood. cSLE presents differently from adults and has a more severe and aggressive course. We describe the clinical and antibody profiles in our cSLE Singapore cohort. All cSLE patients who satisfied the 1997 American College of Rheumatology diagnostic criteria were captured in our lupus registry from January 2009 to January 2014. Data including demographic, cumulative clinical, serologic data, and damage indices were collected. Adjusted mean SLEDAI-2K (AMS) was used to summarize disease activity over multiple visits. Cluster analysis using non-hierarchical K-means procedure was performed on eight selected antibodies. The 64 patients (female:male ratio 5:1; Chinese 45.3%, Malay 28.1%, Indian 9.4%, and other races 17.2%) had a mean onset age of 11.5 years (range 2.1-16.7) and mean age at diagnosis was 11.9 years (range 2.6-18.0). Our study demonstrated differences in clinical manifestations for which hematologic involvement was the most common manifestation with less renal disease and uncommon neurologic manifestation as compared to other cSLE cohorts reported in our region. Antibody clusters were identified in our cohort but their clinical association/discrimination and outcome prediction required further validation study. Outcomes of our cohort in regard to disease activity after therapy and organ damages were comparable if not better to other cSLE cohorts elsewhere. Steroid-related damage, including symptomatic multifocal avascular necrosis and cataract, were not uncommon locally. Infection remains the major cause of death for the continent. Nevertheless, the five year survival rate of our cohort (98.4%) was high.

  2. Global endometrial transcriptomic profiling: transient immune activation precedes tissue proliferation and repair in healthy beef cows

    Directory of Open Access Journals (Sweden)

    Foley Cathriona

    2012-09-01

    Full Text Available Abstract Background All cows experience bacterial contamination and tissue injury in the uterus postpartum, instigating a local inflammatory immune response. However mechanisms that control inflammation and achieve a physiologically functioning endometrium, while avoiding disease in the postpartum cow are not succinctly defined. This study aimed to identify novel candidate genes indicative of inflammation resolution during involution in healthy beef cows. Previous histological analysis of the endometrium revealed elevated inflammation 15 days postpartum (DPP which was significantly decreased by 30 DPP. The current study generated a genome-wide transcriptomic profile of endometrial biopsies from these cows at both time points using mRNA-Seq. The pathway analysis tool GoSeq identified KEGG pathways enriched by significantly differentially expressed genes at both time points. Novel candidate genes associated with inflammatory resolution were subsequently validated in additional postpartum animals using quantitative real-time PCR (qRT-PCR. Results mRNA-Seq revealed 1,107 significantly differentially expressed genes, 73 of which were increased 15 DPP and 1,034 were increased 30 DPP. Early postpartum, enriched immune pathways (adjusted P P SAA1/2, GATA2, IGF1, SHC2, and SERPINA14 genes were significantly elevated 30 DPP and are functionally associated with tissue repair and the restoration of uterine homeostasis postpartum. Conclusions The results of this study reveal an early activation of the immune response which undergoes a temporal functional change toward tissue proliferation and regeneration during endometrial involution in healthy postpartum cows. These molecular changes mirror the activation and resolution of endometrial inflammation during involution previously classified by the degree of neutrophil infiltration. SAA1/2, GATA2, IGF1, SHC2, and SERPINA14 genes may become potential markers for resolution of endometrial inflammation in

  3. Relationship between lipid profile, cardiac hemodynamics status and autoimmune activation markers in patients with ischemic heart disease, accompanied with hypothyroidism

    Directory of Open Access Journals (Sweden)

    N. S. Mykhailovska

    2015-11-01

    Full Text Available Presence of hypothyroidism in patients with coronary heart disease contribute to its progression. Aim. Study purpose was exploring the features of changes in lipid profile, cardiac hemodynamics status, autoimmune activation markers and their relationships in patients with coronary heart disease, accompanied with hypothyroidism. Methods and results. 64 patients were examined. The levels of thyroid-pituitary hormones, concentration of tumor necrosis factor-α, C-reactive protein, endothelin-1, an inhibitor of plasminogen activator-1, neopterin were examined by ELISA. Structural and functional heart features were studied with doppler echocardiography. It was found that patients with coronary heart disease, accompanied with hypothyroidism, had more expressed lipid disorders, heart restructuring such as increased end - diastolic volume, end - diastolic pressure, myocardial mass and stiffness index on the background of immunoinflammatory activation and endothelial dysfunction. Conclusions. It was found correlation between markers of endothelial dysfunction immunoinflammatory activation and lipid profile, heart structural and functional state.

  4. Accelerating VASP electronic structure calculations using graphic processing units

    KAUST Repository

    Hacene, Mohamed

    2012-08-20

    We present a way to improve the performance of the electronic structure Vienna Ab initio Simulation Package (VASP) program. We show that high-performance computers equipped with graphics processing units (GPUs) as accelerators may reduce drastically the computation time when offloading these sections to the graphic chips. The procedure consists of (i) profiling the performance of the code to isolate the time-consuming parts, (ii) rewriting these so that the algorithms become better-suited for the chosen graphic accelerator, and (iii) optimizing memory traffic between the host computer and the GPU accelerator. We chose to accelerate VASP with NVIDIA GPU using CUDA. We compare the GPU and original versions of VASP by evaluating the Davidson and RMM-DIIS algorithms on chemical systems of up to 1100 atoms. In these tests, the total time is reduced by a factor between 3 and 8 when running on n (CPU core + GPU) compared to n CPU cores only, without any accuracy loss. © 2012 Wiley Periodicals, Inc.

  5. Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, Kristen; Sadler, Natalie C.; Wright, Aaron T.; Yeager, Chris; Hyman, Michael R.; Löffler, F. E.

    2016-01-29

    Nitrosomonas europaeais an aerobic nitrifying bacterium that oxidizes ammonia (NH3) to nitrite (NO2) through the sequential activities of ammonia monooxygenase (AMO) and hydroxylamine dehydrogenase (HAO). Many alkynes are mechanism-based inactivators of AMO, and here we describe an activity-based protein profiling method for this enzyme using 1,7-octadiyne (17OD) as a probe. Inactivation of NH4+-dependent O2uptake byN. europaeaby 17OD was time- and concentration-dependent. The effects of 17OD were specific for ammonia-oxidizing activity, andde novoprotein synthesis was required to reestablish this activity after cells were exposed to 17OD. Cells were reacted with Alexa Fluor 647 azide using a copper-catalyzed azide-alkyne cycloaddition (CuAAC) (click) reaction, solubilized, and analyzed by SDS-PAGE and infrared (IR) scanning. A fluorescent 28-kDa polypeptide was observed for cells previously exposed to 17OD but not for cells treated with either allylthiourea or acetylene prior to exposure to 17OD or for cells not previously exposed to 17OD. The fluorescent polypeptide was membrane associated and aggregated when heated with β-mercaptoethanol and SDS. The fluorescent polypeptide was also detected in cells pretreated with other diynes, but not in cells pretreated with structural homologs containing a single ethynyl functional group. The membrane fraction from 17OD-treated cells was conjugated with biotin-azide and solubilized in SDS. Streptavidin affinity-purified polypeptides were on-bead trypsin-digested, and amino acid sequences of the peptide fragments were determined by liquid chromatography-mass spectrometry (LC-MS) analysis. Peptide fragments from AmoA were the predominant peptides detected in 17OD-treated samples. In-gel digestion and

  6. EFFECTS OF INTERFERON THERAPY UPON IMMUNE MARKER PROFILE AND ENZYMATIC ACTIVITY IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH RENAL CANCER

    Directory of Open Access Journals (Sweden)

    L. M. Kurtasova

    2014-01-01

    Full Text Available We have observed forty-four patients with metastatic renal cancer before and after interferon therapy. Immune markers of of peripheral blood lymphocytes were determined by flow cytometry. Activity of NAD (P-dependent dehydrogenase in blood lymphocytes was studied by means of bioluminescence technique. Changes of immune marker profiles and enzymatic activities of peripheral blood lymphocytes were found in patients with renal cancer after a course of interferon therapy.

  7. The offshore Yangsan fault activity in the Quaternary, SE Korea: Analysis of high-resolution seismic profiles

    Science.gov (United States)

    Kim, Han-Joon; Moon, Seonghoon; Jou, Hyeong-Tae; Lee, Gwang Hoon; Yoo, Dong Geun; Lee, Sang Hoon; Kim, Kwang Hee

    2016-12-01

    The NNE-trending dextral Yangsan fault is a > 190-km-long structure in the Korean Peninsula traced to the southeastern coast. The scarcity of Quaternary deposits onland precludes any detailed investigation of the Quaternary activity and structure of the Yangsan fault using seismic reflection profiling. We acquired offshore high-resolution seismic profiles to investigate the extension of the Yangsan fault and constrain its Quaternary activity using stratigraphic markers. The seismic profiles reveal a NNE-trending fault system consisting of a main fault and an array of subsidiary faults that displaced Quaternary sequences. Stratigraphic analysis of seismic profiles indicates that the offshore faults were activated repeatedly in the Quaternary. The up-to-the-east sense of throw on the main fault and plan-view pattern of the fault system are explained by dextral strike-slip faulting. The main fault, when projected toward the Korean Peninsula along its strike, aligns well with the Yangsan fault. We suggest that the offshore fault system is a continuation of the Yangsan fault and has spatial correlation with weak but ongoing seismicity.

  8. Mathematical optimization in computer graphics and vision

    CERN Document Server

    Velho, Luiz; Gomes, Jonas; de Figueiredo, Luiz

    2011-01-01

    Mathematical optimization is used in nearly all computer graphics applications, from computer vision to animation. This book teaches readers the core set of techniques that every computer graphics professional should understand in order to envision and expand the boundaries of what is possible in their work. Study of this authoritative reference will help readers develop a very powerful tool- the ability to create and decipher mathematical models that can better realize solutions to even the toughest problems confronting computer graphics community today.*Distills down a vast a

  9. Real-time graphics rendering engine

    CERN Document Server

    Bao, Hujun

    2011-01-01

    ""Real-Time Graphics Rendering Engine"" reveals the software architecture of the modern real-time 3D graphics rendering engine and the relevant technologies based on the authors' experience developing this high-performance, real-time system. The relevant knowledge about real-time graphics rendering such as the rendering pipeline, the visual appearance and shading and lighting models are also introduced. This book is intended to offer well-founded guidance for researchers and developers who are interested in building their own rendering engines. Hujun Bao is a professor at the State Key Lab of

  10. Characterizing C6+P2-graphic Sequences

    Institute of Scientific and Technical Information of China (English)

    HU Li-li

    2014-01-01

    For a given graph H, a graphic sequence π = (d1, d2, · · · , dn) is said to be potentially H-graphic if π has a realization containing H as a subgraph. In this paper, we characterize the potentially C6 +P2-graphic sequences where C6 +P2 denotes the graph obtained from C6 by adding two adjacent edges to the three pairwise nonadjacent vertices of C6. Moreover, we use the characterization to determine the value ofσ(C6+P2, n).

  11. A conceptual framework for a sports knee injury performance profile (SKIPP and return to activity criteria (RTAC

    Directory of Open Access Journals (Sweden)

    David Logerstedt

    2015-10-01

    Full Text Available ABSTRACTInjuries to the knee, including intra-articular fractures, ligamentous ruptures, and meniscal and articular cartilage lesions, are commonplace within sports. Despite advancements in surgical techniques and enhanced rehabilitation, athletes returning to cutting, pivoting, and jumping sports after a knee injury are at greater risk of sustaining a second injury. The clinical utility of objective criteria presents a decision-making challenge to ensure athletes are fully rehabilitated and safe to return to sport. A system centered on specific indicators that can be used to develop a comprehensive profile to monitor rehabilitation progression and to establish return to activity criteria is recommended to clear athletes to begin a progressive and systematic approach to activities and sports. Integration of a sports knee injury performance profile with return to activity criteria can guide clinicians in facilitating an athlete's safe return to sport, prevention of subsequent injury, and life-long knee joint health.

  12. A conceptual framework for a sports knee injury performance profile (SKIPP) and return to activity criteria (RTAC)

    Science.gov (United States)

    Logerstedt, David; Arundale, Amelia; Lynch, Andrew; Snyder-Mackler, Lynn

    2015-01-01

    Injuries to the knee, including intra-articular fractures, ligamentous ruptures, and meniscal and articular cartilage lesions, are commonplace within sports. Despite advancements in surgical techniques and enhanced rehabilitation, athletes returning to cutting, pivoting, and jumping sports after a knee injury are at greater risk of sustaining a second injury. The clinical utility of objective criteria presents a decision-making challenge to ensure athletes are fully rehabilitated and safe to return to sport. A system centered on specific indicators that can be used to develop a comprehensive profile to monitor rehabilitation progression and to establish return to activity criteria is recommended to clear athletes to begin a progressive and systematic approach to activities and sports. Integration of a sports knee injury performance profile with return to activity criteria can guide clinicians in facilitating an athlete's safe return to sport, prevention of subsequent injury, and life-long knee joint health. PMID:26537805

  13. ENZYMATIC ACTIVITY AND ANTIBIOTIC RESISTANCE PROFILE OF LACTOBACILLUS PARACASEI SSP. PARACASEI-1 ISOLATED FROM REGIONAL YOGURTS OF BANGLADESH

    Directory of Open Access Journals (Sweden)

    Ummay Honi

    2013-12-01

    Full Text Available Lactobacillus paracasei ssp. paracasei-1 was identified from traditional yogurts of Khulna region, Bangladesh and its enzyme and antibiotic resistance profiles were determined. A commercially available API Zym kit was employed to determine the activities of 19 different enzymes. We found that L. paracasei ssp. paracasei-1 showed strong activities for several enzymes, viz. leucine arylamidase, valine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, α –Glucosidase, N-Acetyl- β- glucosaminidase while activities for other enzymes were absent. Antibiotic resistance profile was assessed by minimum inhibitory concentration (MIC test for 61 major antibiotics and 4 antifungal agents obtained from commercial sources in MRS Agar media. The strain generally showed resistance to gram negative spectrum antibiotic while it showed susceptibility towards β-lactam antibiotic to gram positive spectrum antibiotic. The findings provide the therapeutic basis of using L. paracasei ssp. paracasei-1 in finished food products.

  14. Evaluating the Safety Profile of Non-Active Implantable Medical Devices Compared with Medicines.

    Science.gov (United States)

    Pane, Josep; Coloma, Preciosa M; Verhamme, Katia M C; Sturkenboom, Miriam C J M; Rebollo, Irene

    2017-01-01

    Recent safety issues involving non-active implantable medical devices (NAIMDs) have highlighted the need for better pre-market and post-market evaluation. Some stakeholders have argued that certain features of medicine safety evaluation should also be applied to medical devices. Our objectives were to compare the current processes and methodologies for the assessment of NAIMD safety profiles with those for medicines, identify potential gaps, and make recommendations for the adoption of new methodologies for the ongoing benefit-risk monitoring of these devices throughout their entire life cycle. A literature review served to examine the current tools for the safety evaluation of NAIMDs and those for medicines. We searched MEDLINE using these two categories. We supplemented this search with Google searches using the same key terms used in the MEDLINE search. Using a comparative approach, we summarized the new product design, development cycle (preclinical and clinical phases), and post-market phases for NAIMDs and drugs. We also evaluated and compared the respective processes to integrate and assess safety data during the life cycle of the products, including signal detection, signal management, and subsequent potential regulatory actions. The search identified a gap in NAIMD safety signal generation: no global program exists that collects and analyzes adverse events and product quality issues. Data sources in real-world settings, such as electronic health records, need to be effectively identified and explored as additional sources of safety information, particularly in some areas such as the EU and USA where there are plans to implement the unique device identifier (UDI). The UDI and other initiatives will enable more robust follow-up and assessment of long-term patient outcomes. The safety evaluation system for NAIMDs differs in many ways from those for drugs, but both systems face analogous challenges with respect to monitoring real-world usage. Certain features

  15. Methane related changes in prokaryotic activity along geochemical profiles in sediments of Lake Kinneret (Israel)

    Science.gov (United States)

    Bar Or, I.; Ben-Dov, E.; Kushmaro, A.; Eckert, W.; Sivan, O.

    2014-06-01

    Microbial methane oxidation process (methanotrophy) is the primary control on the emission of the greenhouse gas methane (CH4) to the atmosphere. In terrestrial environments, aerobic methanotrophic bacteria are mainly responsible for oxidizing the methane. In marine sediments the coupling of the anaerobic oxidation of methane (AOM) with sulfate reduction, often by a consortium of anaerobic methanotrophic archaea (ANME) and sulfate reducing bacteria, was found to consume almost all the upward diffusing methane. Recently, we showed geochemical evidence for AOM driven by iron reduction in Lake Kinneret (LK) (Israel) deep sediments and suggested that this process can be an important global methane sink. The goal of the present study was to link the geochemical gradients found in the porewater (chemical and isotope profiles) with possible changes in microbial community structure. Specifically, we examined the possible shift in the microbial community in the deep iron-driven AOM zone and its similarity to known sulfate driven AOM populations. Screening of archaeal 16S rRNA gene sequences revealed Thaumarchaeota and Euryarchaeota as the dominant phyla in the sediment. Thaumarchaeota, which belongs to the family of copper containing membrane-bound monooxgenases, increased with depth while Euryarchaeota decreased. This may indicate the involvement of Thaumarchaeota, which were discovered to be ammonia oxidizers but whose activity could also be linked to methane, in AOM in the deep sediment. ANMEs sequences were not found in the clone libraries, suggesting that iron-driven AOM is not through sulfate. Bacterial 16S rRNA sequences displayed shifts in community diversity with depth. Proteobacteria and Chloroflexi increased with depth, which could be connected with their different dissimilatory anaerobic processes. The observed changes in microbial community structure suggest possible direct and indirect mechanisms for iron-driven AOM in deep sediments.

  16. Solar energy in Argentina: a profile of renewable energy activity in its national context

    Energy Technology Data Exchange (ETDEWEB)

    Hawkins, D.

    1981-01-01

    The following subjects are included: the country overview; the energy summary; the geopolitical, economic, and cultural aspects of the Republic of Argentina; the energy profile; and international contacts, manufacturers, and projects. (MHR)

  17. Proteomic profiling of patient-derived glioblastoma xenografts identifies a subset with activated EGFR: implications for drug development.

    Science.gov (United States)

    Brown, Kristine E; Chagoya, Gustavo; Kwatra, Shawn G; Yen, Timothy; Keir, Stephen T; Cooter, Mary; Hoadley, Katherine A; Rasheed, Ahmed; Lipp, Eric S; Mclendon, Roger; Ali-Osman, Francis; Bigner, Darell D; Sampson, John H; Kwatra, Madan M

    2015-06-01

    The development of drugs to inhibit glioblastoma (GBM) growth requires reliable pre-clinical models. To date, proteomic level validation of widely used patient-derived glioblastoma xenografts (PDGX) has not been performed. In the present study, we characterized 20 PDGX models according to subtype classification based on The Cancer Genome Atlas criteria, TP53, PTEN, IDH 1/2, and TERT promoter genetic analysis, EGFR amplification status, and examined their proteomic profiles against those of their parent tumors. The 20 PDGXs belonged to three of four The Cancer Genome Atlas subtypes: eight classical, eight mesenchymal, and four proneural; none neural. Amplification of EGFR gene was observed in 9 of 20 xenografts, and of these, 3 harbored the EGFRvIII mutation. We then performed proteomic profiling of PDGX, analyzing expression/activity of several proteins including EGFR. Levels of EGFR phosphorylated at Y1068 vary considerably between PDGX samples, and this pattern was also seen in primary GBM. Partitioning of 20 PDGX into high (n = 5) and low (n = 15) groups identified a panel of proteins associated with high EGFR activity. Thus, PDGX with high EGFR activity represent an excellent pre-clinical model to develop therapies for a subset of GBM patients whose tumors are characterized by high EGFR activity. Further, the proteins found to be associated with high EGFR activity can be monitored to assess the effectiveness of targeting EGFR. The development of drugs to inhibit glioblastoma (GBM) growth requires reliable pre-clinical models. We validated proteomic profiles using patient-derived glioblastoma xenografts (PDGX), characterizing 20 PDGX models according to subtype classification based on The Cancer Genome Atlas (TCGA) criteria, TP53, PTEN, IDH 1/2, and TERT promoter genetic analysis, EGFR amplification status, and examined their proteomic profiles against those of their parent tumors. Proteins found to be associated with high EGFR activity represent potential

  18. Motivational Profiles for Physical Activity Practice in Adults with Type 2 Diabetes: A Self-Determination Theory Perspective.

    Science.gov (United States)

    Gourlan, Mathieu; Trouilloud, David; Boiché, Julie

    2016-01-01

    Drawing on Self-Determination Theory, this study explored the motivational profiles toward Physical Activity (PA) among adults with type 2 diabetes and the relationships between motivational profile, perceived competence and PA. Participants were 350 men and women (Mean age 62.77 years) who were interviewed on their motivations toward PA, perceived level of competence to practice, and PA practice. Cluster analyses reveal the existence of three distinct profiles: "High Combined" (ie, high scores on motivations ranging from intrinsic to external regulation, moderate level on amotivation), "Self-Determined" (ie, high scores on intrinsic, integrated, and identified regulations; low scores on other regulations), and "Moderate" (ie, moderate scores on all regulations). Participants with "High Combined" and "Self-Determined" profiles reported higher perceived competence and longer leisure-time PA practice in comparison to those with a "Moderate" profile. This study highlights the necessity of adopting a person-centered approach to better understand motivation toward PA among type 2 diabetics.

  19. PLM-COMPETENCE FORMATION USING COMPUTATIONAL-GRAPHICAL PRACTICE

    Directory of Open Access Journals (Sweden)

    Vladimir V. Zelentsov

    2014-01-01

    Full Text Available The description of a new kind of learning activity “Computational - graphical practice” is presented. This practice aimed at consolidating the knowledge and skills acquired by students in the study of the basic engineering disciplines and bridge the gap in the training of students in the field of PLM-technology. The goals, objectives, program practices and competencies, which should have a student after passing the training practice, are listed 

  20. Learning Undirected Graphical Models with Structure Penalty

    CERN Document Server

    Ding, Shilin

    2011-01-01

    In undirected graphical models, learning the graph structure and learning the functions that relate the predictive variables (features) to the responses given the structure are two topics that have been widely investigated in machine learning and statistics. Learning graphical models in two stages will have problems because graph structure may change after considering the features. The main contribution of this paper is the proposed method that learns the graph structure and functions on the graph at the same time. General graphical models with binary outcomes conditioned on predictive variables are proved to be equivalent to multivariate Bernoulli model. The reparameterization of the potential functions in graphical model by conditional log odds ratios in multivariate Bernoulli model offers advantage in the representation of the conditional independence structure in the model. Additionally, we impose a structure penalty on groups of conditional log odds ratios to learn the graph structure. These groups of fu...

  1. Realtime multi-plot graphics system

    Science.gov (United States)

    Shipkowski, Michael S.

    1990-01-01

    The increased complexity of test operations and customer requirements at Langley Research Center's National Transonic Facility (NTF) surpassed the capabilities of the initial realtime graphics system. The analysis of existing hardware and software and the enhancements made to develop a new realtime graphics system are described. The result of this effort is a cost effective system, based on hardware already in place, that support high speed, high resolution, generation and display of multiple realtime plots. The enhanced graphics system (EGS) meets the current and foreseeable future realtime graphics requirements of the NTF. While this system was developed to support wind tunnel operations, the overall design and capability of the system is applicable to other realtime data acquisition systems that have realtime plot requirements.

  2. Group Design Problems in Engineering Design Graphics.

    Science.gov (United States)

    Kelley, David

    2001-01-01

    Describes group design techniques used within the engineering design graphics sequence at Western Washington University. Engineering and design philosophies such as concurrent engineering place an emphasis on group collaboration for the solving of design problems. (Author/DDR)

  3. History And Importance Of Graphic Design

    OpenAIRE

    Lyallya, Kirill

    2016-01-01

    This thesis is about history and importance of graphic design in different periods, from ancient times until today. The features inherent in different countries are considered. The techniques, basic methods for creating projects and computer software that designers have used are mentioned. In order to understand the importance of graphic design in our lives, it is considered from the side of ordinary people, how it manifests itself in daily lives and how it affects business. The thesis provid...

  4. Alarm annunciation in a graphical environment

    Energy Technology Data Exchange (ETDEWEB)

    Adams, D.G.

    1994-08-01

    Well-designed graphical user interfaces, such as Microsoft Windows{trademark} or UNIX{trademark} -- based X-Windows, provide a capability for enhanced display of security alarm information. Conversely, a poorly designed interface can quickly overwhelm an operator. This paper describes types of graphical information that can be displayed and offers guidance on how to best display that information. Limits are proposed for the complexity of the user interface, and guidelines are suggested for the display of maps and sensors.

  5. Discrimination of differentially inhibited cysteine proteases by activity-based profiling using cystatin variants with tailored specificities.

    Science.gov (United States)

    Sainsbury, Frank; Rhéaume, Ann-Julie; Goulet, Marie-Claire; Vorster, Juan; Michaud, Dominique

    2012-12-01

    Recent research has shown the possibility of tailoring the inhibitory specificity of plant cystatins toward cysteine (Cys) proteases by single mutations at positively selected amino acid sites. Here we devised a cystatin activity-based profiling approach to assess the impact of such mutations at the proteome scale using single variants of tomato cystatin SlCYS8 and digestive Cys proteases of the herbivorous insect, Colorado potato beetle, as a model. Biotinylated forms of SlCYS8 and SlCYS8 variants were used to capture susceptible Cys proteases in insect midgut protein extracts by biotin immobilization on avidin-embedded beads. A quantitative LC-MS/MS analysis of the captured proteins was performed to compare the inhibitory profile of different SlCYS8 variants. The approach confirmed the relevance of phylogenetic inferences categorizing the insect digestive Cys proteases into six functionally distinct families. It also revealed significant variation in protease family profiles captured with N-terminal variants of SlCYS8, in line with in silico structural models for Cys protease-SlCYS8 interactions suggesting a functional role for the N-terminal region. Our data confirm overall the usefulness of cystatin activity-based protease profiling for the monitoring of Cys protease-inhibitor interactions in complex biological systems. They also illustrate the potential of biotinylated cystatins to identify recombinant cystatin candidates for the inactivation of specific Cys protease targets.

  6. Enhancing graphical literacy skills in the high school science classroom via authentic, intensive data collection and graphical representation exposure

    Science.gov (United States)

    Palmeri, Anthony

    This research project was developed to provide extensive practice and exposure to data collection and data representation in a high school science classroom. The student population engaged in this study included 40 high school sophomores enrolled in two microbiology classes. Laboratory investigations and activities were deliberately designed to include quantitative data collection that necessitated organization and graphical representation. These activities were embedded into the curriculum and conducted in conjunction with the normal and expected course content, rather than as a separate entity. It was expected that routine practice with graph construction and interpretation would result in improved competency when graphing data and proficiency in analyzing graphs. To objectively test the effectiveness in achieving this goal, a pre-test and post-test that included graph construction, interpretation, interpolation, extrapolation, and analysis was administered. Based on the results of a paired T-Test, graphical literacy was significantly enhanced by extensive practice and exposure to data representation.

  7. Profile-QSAR: a novel meta-QSAR method that combines activities across the kinase family to accurately predict affinity, selectivity, and cellular activity.

    Science.gov (United States)

    Martin, Eric; Mukherjee, Prasenjit; Sullivan, David; Jansen, Johanna

    2011-08-22

    Profile-QSAR is a novel 2D predictive model building method for kinases. This "meta-QSAR" method models the activity of each compound against a new kinase target as a linear combination of its predicted activities against a large panel of 92 previously studied kinases comprised from 115 assays. Profile-QSAR starts with a sparse incomplete kinase by compound (KxC) activity matrix, used to generate Bayesian QSAR models for the 92 "basis-set" kinases. These Bayesian QSARs generate a complete "synthetic" KxC activity matrix of predictions. These synthetic activities are used as "chemical descriptors" to train partial-least squares (PLS) models, from modest amounts of medium-throughput screening data, for predicting activity against new kinases. The Profile-QSAR predictions for the 92 kinases (115 assays) gave a median external R²(ext) = 0.59 on 25% held-out test sets. The method has proven accurate enough to predict pairwise kinase selectivities with a median correlation of R²(ext) = 0.61 for 958 kinase pairs with at least 600 common compounds. It has been further expanded by adding a "C(k)XC" cellular activity matrix to the KxC matrix to predict cellular activity for 42 kinase driven cellular assays with median R²(ext) = 0.58 for 24 target modulation assays and R²(ext) = 0.41 for 18 cell proliferation assays. The 2D Profile-QSAR, along with the 3D Surrogate AutoShim, are the foundations of an internally developed iterative medium-throughput screening (IMTS) methodology for virtual screening (VS) of compound archives as an alternative to experimental high-throughput screening (HTS). The method has been applied to 20 actual prospective kinase projects. Biological results have so far been obtained in eight of them. Q² values ranged from 0.3 to 0.7. Hit-rates at 10 uM for experimentally tested compounds varied from 25% to 80%, except in K5, which was a special case aimed specifically at finding "type II" binders, where none of the compounds were predicted to be

  8. A Linux Workstation for High Performance Graphics

    Science.gov (United States)

    Geist, Robert; Westall, James

    2000-01-01

    The primary goal of this effort was to provide a low-cost method of obtaining high-performance 3-D graphics using an industry standard library (OpenGL) on PC class computers. Previously, users interested in doing substantial visualization or graphical manipulation were constrained to using specialized, custom hardware most often found in computers from Silicon Graphics (SGI). We provided an alternative to expensive SGI hardware by taking advantage of third-party, 3-D graphics accelerators that have now become available at very affordable prices. To make use of this hardware our goal was to provide a free, redistributable, and fully-compatible OpenGL work-alike library so that existing bodies of code could simply be recompiled. for PC class machines running a free version of Unix. This should allow substantial cost savings while greatly expanding the population of people with access to a serious graphics development and viewing environment. This should offer a means for NASA to provide a spectrum of graphics performance to its scientists, supplying high-end specialized SGI hardware for high-performance visualization while fulfilling the requirements of medium and lower performance applications with generic, off-the-shelf components and still maintaining compatibility between the two.

  9. Perception of tactile graphics: embossings versus cutouts.

    Science.gov (United States)

    Kalia, Amy; Hopkins, Rose; Jin, David; Yazzolino, Lindsay; Verma, Svena; Merabet, Lotfi; Phillips, Flip; Sinha, Pawan

    2014-01-01

    Graphical information, such as illustrations, graphs, and diagrams, are an essential complement to text for conveying knowledge about the world. Although graphics can be communicated well via the visual modality, conveying this information via touch has proven to be challenging. The lack of easily comprehensible tactile graphics poses a problem for the blind. In this paper, we advance a hypothesis for the limited effectiveness of tactile graphics. The hypothesis contends that conventional graphics that rely upon embossings on two-dimensional surfaces do not allow the deployment of tactile exploratory procedures that are crucial for assessing global shape. Besides potentially accounting for some of the shortcomings of current approaches, this hypothesis also serves a prescriptive purpose by suggesting a different strategy for conveying graphical information via touch, one based on cutouts. We describe experiments demonstrating the greater effectiveness of this approach for conveying shape and identity information. These results hold the potential for creating more comprehensible tactile drawings for the visually impaired while also providing insights into shape estimation processes in the tactile modality.

  10. Graphic Notation in Music Therapy: A Discussion of What to Notate in Graphic Notation and How

    DEFF Research Database (Denmark)

    Bergstrøm-Nielsen, Carl

    2009-01-01

    This article presents graphic notations of music and related forms of communication in music therapy contexts, created by different authors and practitioners. Their purposes, objects of description, and the elements of graphic language are reflected upon in a comparative discussion. From...... are also important concerns. Among the authors discussed, there is a large variety both in goals and methods. Keywords are proposed to circumscribe moments of possible interest connected to graphic notations. I suggest that the discipline of graphic notation can be useful for the grounding of music therapy...... presentation and research in empirical, clinical musical reality, and welcome further discussion and explorative work....

  11. Profiles of exercise motivation, physical activity, exercise habit, and academic performance in Malaysian adolescents: A cluster analysis

    OpenAIRE

    Hairul Anuar Hashim; Freddy Golok; Rosmatunisah Ali

    2011-01-01

    Objectives: This study examined Malaysian adolescents’ profiles of exercise motivation, exercise habit strength, academic performance, and levels of physical activity (PA) using cluster analysis.Methods: The sample (n = 300) consisted of 65.6% males and 34.4% females with a mean age of 13.40 ± 0.49. Statistical analysis was performed using cluster analysis.Results: Cluster analysis revealed three distinct cluster groups. Cluster 1 is characterized by a moderate level of PA, relatively high in...

  12. HCl and ClO in activated Arctic air; first retrieved vertical profiles from TELIS submillimetre limb spectra

    Directory of Open Access Journals (Sweden)

    A. de Lange

    2012-02-01

    Full Text Available The first profile retrieval results of the Terahertz and submillimeter Limb Sounder (TELIS balloon instrument are presented. The spectra are recorded during a 13-h balloon flight on 24 January 2010 from Kiruna, Sweden. The TELIS instrument was mounted on the MIPAS-B2 gondola and shared this platform with the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS and the mini-Differential Optical Absorption Spectroscopy (mini-DOAS instruments. The flight took place within the Arctic vortex at an altitude of ≈34 km in chlorine activated air, and both active (ClO and inactive chlorine (HCl were measured over an altitude range of respectively ≈16–32 km and ≈10–32 km. In this altitude range, the increase of ClO concentration levels during sunrise has been recorded with a temporal resolution of one minute. During the daytime equilibrium, a maximum ClO level of 2.1 ± 0.3 ppbv has been observed at an altitude of 23.5 km. This equilibrium profile is validated against the ClO profile by the satellite instrument Microwave Limb Sounder (MLS aboard EOS Aura. HCl profiles have been determined from two different isotopes – H35Cl and H37Cl – and are also validated against MLS. The precision of all profiles is well below 0.01 ppbv and the overall accuracy is therefore governed by systematic effects. The total uncertainty of these effects is estimated to be maximal 0.3 ppbv for ClO around its peak value at 23.5 km during the daytime equilibrium, and for HCl it ranges from 0.05 to 0.4 ppbv, depending on altitude. In both cases the main uncertainty stems from a largely unknown non-linear response in the detector.

  13. X-ray Surface Brightness Profiles of Active Galactic Nuclei in the Extended Groth Strip: Implications for AGN Feedback

    CERN Document Server

    Chatterjee, Suchetana; Jeltema, Tesla; Myers, Adam D; Aird, James; Coil, Alison L; Cooper, Michael; Finoguenov, Alexis; Laird, Elise; Montero-Dorta, Antonio; Nandra, Kripal; Willmer, Christopher; Yan, Renbin

    2013-01-01

    Using data from the All Wavelength Extended Groth Strip International Survey (AEGIS) we statistically detect the extended X-ray emission in the interstellar medium (ISM) in both active and normal galaxies at 0.3 < z < 1.3 at a scale of 40-60 kpc. We study the effect of feedback from active galactic nuclei (AGN) on the diffuse interstellar gas by comparing the stacked X-ray surface brightness profiles of active and normal galaxies in the same redshift range with identical properties in optical color--magnitude space. In accordance with theoretical studies we detect a slight deficit (< 1.5 \\sigma) of X-ray photons when averaged over a scale of 0-30 kpc in the profile of AGN host galaxies at 0.3 < z < 0.7. The equivalent flux deficit is (1.25 +/- 0.75) X 10^(-19) ergs/s/cm^(-2). When averaged over a scale of 30-60 kpc, beyond the PSF scales of our AGN sources, we observe a (~ 2 \\sigma) photon excess in the profile of the AGN host galaxies with an equivalent flux excess of (1.1 +/- 0.5) X 10^{-19} ...

  14. Prevalence of human cell material: DNA and RNA profiling of public and private objects and after activity scenarios.

    Science.gov (United States)

    van den Berge, M; Ozcanhan, G; Zijlstra, S; Lindenbergh, A; Sijen, T

    2016-03-01

    Especially when minute evidentiary traces are analysed, background cell material unrelated to the crime may contribute to detectable levels in the genetic analyses. To gain understanding on the composition of human cell material residing on surfaces contributing to background traces, we performed DNA and mRNA profiling on samplings of various items. Samples were selected by considering events contributing to cell material deposits in exemplary activities (e.g. dragging a person by the trouser ankles), and can be grouped as public objects, private samples, transfer-related samples and washing machine experiments. Results show that high DNA yields do not necessarily relate to an increased number of contributors or to the detection of other cell types than skin. Background cellular material may be found on any type of public or private item. When a major contributor can be deduced in DNA profiles from private items, this can be a different person than the owner of the item. Also when a specific activity is performed and the areas of physical contact are analysed, the "perpetrator" does not necessarily represent the major contributor in the STR profile. Washing machine experiments show that transfer and persistence during laundry is limited for DNA and cell type dependent for RNA. Skin conditions such as the presence of sebum or sweat can promote DNA transfer. Results of this study, which encompasses 549 samples, increase our understanding regarding the prevalence of human cell material in background and activity scenarios.

  15. Polypharmacology directed compound data mining: identification of promiscuous chemotypes with different activity profiles and comparison to approved drugs.

    Science.gov (United States)

    Hu, Ye; Bajorath, Jürgen

    2010-12-27

    Increasing evidence that many pharmaceutically relevant compounds elicit their effects through binding to multiple targets, so-called polypharmacology, is beginning to change conventional drug discovery and design strategies. In light of this paradigm shift, we have mined publicly available compound and bioactivity data for promiscuous chemotypes. For this purpose, a hierarchy of active compounds, atomic property based scaffolds, and unique molecular topologies were generated, and activity annotations were analyzed using this framework. Starting from ∼35 000 compounds active against human targets with at least 1 μM potency, 33 chemotypes with distinct topology were identified that represented molecules active against at least 3 different target families. Network representations were utilized to study scaffold-target family relationships and activity profiles of scaffolds corresponding to promiscuous chemotypes. A subset of promiscuous chemotypes displayed a significant enrichment in drugs over bioactive compounds. A total of 190 drugs were identified that had on average only 2 known target annotations but belonged to the 7 most promiscuous chemotypes that were active against 8-15 target families. These drugs should be attractive candidates for polypharmacological profiling.

  16. Assessing Sources of Stress to Aquatic Ecosystems: Using Biomarkers and Bioindicators to Characterize Exodure-Response Profiles of Anthropogenic Activities

    Energy Technology Data Exchange (ETDEWEB)

    Adams, S.M.

    1999-03-29

    Establishing causal relationships between sources of environmental stressors and aquatic ecosystem health if difficult because of the many biotic and abiotic factors which can influence or modify responses of biological systems to stress, the orders of magnitude involved in extrapolation over both spatial and temporal scales, and compensatory mechanisms such as density-dependent responses that operate in populations. To address the problem of establishing causality between stressors and effects on aquatic systems, a diagnostic approach, based on exposure-response profiles for various anthropogenic activities, was developed to help identify sources of stress responsible for effects on aquatic systems at ecological significant levels of biological organization (individual, population, community). To generate these exposure-effects profiles, biomarkers of exposure were plotted against bioindicators of corresponding effects for several major anthropogenic activities including petrochemical , pulp and paper, domestic sewage, mining operations, land-development activities, and agricultural activities. Biomarkers of exposure to environmental stressors varied depending on the type of anthropogenic activity involved. Bioindicator effects, however, including histopathological lesions, bioenergetic status, individual growth, reproductive impairment, and community-level responses were similar among many of the major anthropogenic activities. This approach is valuable to help identify and diagnose sources of stressors in environments impacted by multiple stressors. By identifying the types and sources of environmental stressors, aquatic ecosystems can be more effectively protected and managed to maintain acceptable levels of environmental quality and ecosystem fitness.

  17. Dynamic Resource Access Using Graphical Game in Asymmetric Wireless Networks

    Directory of Open Access Journals (Sweden)

    Fangwei Li

    2013-08-01

    Full Text Available In order to improve the resource utilization in asymmetric wireless networks, a novel dynamic resource access algorithm was presented. As the asymmetry of information and the locality of users' actions in distributed wireless networks, the resource access problem was expressed as a simple graphical game model. Let the graphic topology indicate the internal game structure of the realistic environment. Then the Nash equilibrium was got by minimizing the individual regret instead of the system regret. The proposed algorithm realized efficient resource access through exchanging the active information and regret in the competitive community. Theoretical analysis and simulation results show that the algorithm can converge to a suitable pure strategy Nash equilibrium point quickly with less amount of calculation, avoids conflict effectively, and improves the system capacity and power utilization especially in the condition of insufficient resources

  18. Solar energy in Italy: a profile of renewable energy activity in its national context

    Energy Technology Data Exchange (ETDEWEB)

    Shea, C.A.

    1980-12-01

    The following are included: country overview; energy summary; Italian Republic-geopolitical, economic, and cultural aspects; the energy profile; imported energy sources; solar energy research and development; solar energy organizations; solar energy related legislation and administration policies; and international agreements, contacts, manufacturers, and projects. (MHR)

  19. Line profiles of the two-phase medium broad-line region in active galactic nuclei

    Institute of Scientific and Technical Information of China (English)

    薛随建; 林宣滨; 程福臻; John Kwan

    1996-01-01

    In the framework of BLR in AGNs, i.e. large number of small, cold and dense emission-line clouds immerging in an intercloud hot medium, a delicate radiation model for a typical emission cloud is established, in which the backside photoionization of the cloud against the central continuum source by the scattered diffuse continuum in the hot medium is considered. Under the assumption of the radial kinematics of the BLR clouds, the line profiles for the two important UV emission-lines, Lya and CIV/11549 are computed; and based on these calculations, various physical factors that affect the properties of the emission-line profiles are discussed. It is concluded that: (i) when the BLR clouds is inflow, there exists a confining hot medium with density around 2 x 105 cm-3. In this case, theoretical line profiles agree well with the observational; (ii) both symmetric Lya and nearly symmetric CIV line profiles show blue velocity shifts, the quantities of which are sensitive to the cutoff of the outer radius of t

  20. Energy in Mexico: a profile of solar energy activity in its national context

    Energy Technology Data Exchange (ETDEWEB)

    Hawkins, D.

    1980-04-01

    The geopolitical, economic, and cultural aspects of the United States of Mexico are presented. Mexico's energy profile includes the following: energy policy objectives, government energy structure, organizations for implementation, indigeneous energy sources, imported energy sources, solar energy research and development, solar energy organizations and solar energy related legislation and administrative policies. International agreements, contacts, manufacturers, and projects are listed. (MRH)

  1. Modelling of JET diagnostics using Bayesian Graphical Models

    Energy Technology Data Exchange (ETDEWEB)

    Svensson, J. [IPP Greifswald, Greifswald (Germany); Ford, O. [Imperial College, London (United Kingdom); McDonald, D.; Hole, M.; Nessi, G. von; Meakins, A.; Brix, M.; Thomsen, H.; Werner, A.; Sirinelli, A.

    2011-07-01

    The mapping between physics parameters (such as densities, currents, flows, temperatures etc) defining the plasma 'state' under a given model and the raw observations of each plasma diagnostic will 1) depend on the particular physics model used, 2) is inherently probabilistic, from uncertainties on both observations and instrumental aspects of the mapping, such as calibrations, instrument functions etc. A flexible and principled way of modelling such interconnected probabilistic systems is through so called Bayesian graphical models. Being an amalgam between graph theory and probability theory, Bayesian graphical models can simulate the complex interconnections between physics models and diagnostic observations from multiple heterogeneous diagnostic systems, making it relatively easy to optimally combine the observations from multiple diagnostics for joint inference on parameters of the underlying physics model, which in itself can be represented as part of the graph. At JET about 10 diagnostic systems have to date been modelled in this way, and has lead to a number of new results, including: the reconstruction of the flux surface topology and q-profiles without any specific equilibrium assumption, using information from a number of different diagnostic systems; profile inversions taking into account the uncertainties in the flux surface positions and a substantial increase in accuracy of JET electron density and temperature profiles, including improved pedestal resolution, through the joint analysis of three diagnostic systems. It is believed that the Bayesian graph approach could potentially be utilised for very large sets of diagnostics, providing a generic data analysis framework for nuclear fusion experiments, that would be able to optimally utilize the information from multiple diagnostics simultaneously, and where the explicit graph representation of the connections to underlying physics models could be used for sophisticated model testing. This

  2. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity.

    Science.gov (United States)

    Kontogianni, Vassiliki G; Tomic, Goran; Nikolic, Ivana; Nerantzaki, Alexandra A; Sayyad, Nisar; Stosic-Grujicic, Stanislava; Stojanovic, Ivana; Gerothanassis, Ioannis P; Tzakos, Andreas G

    2013-01-01

    The goal of this study was to monitor the anti-proliferative activity of Rosmarinus officinalis and Salvia officinalis extracts against cancer cells and to correlate this activity with their phytochemical profiles using liquid chromatography/diode array detection/electrospray ion trap tandem mass spectrometry (LC/DAD/ESI-MS(n)). For the quantitative estimation of triterpenic acids in the crude extracts an NMR based methodology was used and compared with the HPLC measurements, both applied for the first time, for the case of betulinic acid. Both extracts exerted cytotoxic activity through dose-dependent impairment of viability and mitochondrial activity of rat insulinoma m5F (RINm5F) cells. Decrease of RINm5F viability was mediated by nitric oxide (NO)-induced apoptosis. Importantly, these extracts potentiated NO and TNF-α release from macrophages therefore enhancing their cytocidal action. The rosemary extract developed more pronounced antioxidant, cytotoxic and immunomodifying activities, probably due to the presence of betulinic acid and a higher concentration of carnosic acid in its phytochemical profile.

  3. In vitro anti-plasmodial activity of Dicoma anomala subsp. gerrardii (Asteraceae: identification of its main active constituent, structure-activity relationship studies and gene expression profiling

    Directory of Open Access Journals (Sweden)

    van Heerden Fanie R

    2011-10-01

    Full Text Available Abstract Background Anti-malarial drug resistance threatens to undermine efforts to eliminate this deadly disease. The resulting omnipresent requirement for drugs with novel modes of action prompted a national consortium initiative to discover new anti-plasmodial agents from South African medicinal plants. One of the plants selected for investigation was Dicoma anomala subsp. gerrardii, based on its ethnomedicinal profile. Methods Standard phytochemical analysis techniques, including solvent-solvent extraction, thin-layer- and column chromatography, were used to isolate the main active constituent of Dicoma anomala subsp. gerrardii. The crystallized pure compound was identified using nuclear magnetic resonance spectroscopy, mass spectrometry and X-ray crystallography. The compound was tested in vitro on Plasmodium falciparum cultures using the parasite lactate dehydrogenase (pLDH assay and was found to have anti-malarial activity. To determine the functional groups responsible for the activity, a small collection of synthetic analogues was generated - the aim being to vary features proposed as likely to be related to the anti-malarial activity and to quantify the effect of the modifications in vitro using the pLDH assay. The effects of the pure compound on the P. falciparum transcriptome were subsequently investigated by treating ring-stage parasites (alongside untreated controls, followed by oligonucleotide microarray- and data analysis. Results The main active constituent was identified as dehydrobrachylaenolide, a eudesmanolide-type sesquiterpene lactone. The compound demonstrated an in vitro IC50 of 1.865 μM against a chloroquine-sensitive strain (D10 of P. falciparum. Synthetic analogues of the compound confirmed an absolute requirement that the α-methylene lactone be present in the eudesmanolide before significant anti-malarial activity was observed. This feature is absent in the artemisinins and suggests a different mode of action

  4. Numerical Integration with Graphical Processing Unit for QKD Simulation

    Science.gov (United States)

    2014-03-27

    existing and proposed Quantum Key Distribution (QKD) systems. This research investigates using graphical processing unit ( GPU ) technology to more...Time Pad GPU graphical processing unit API application programming interface CUDA Compute Unified Device Architecture SIMD single-instruction-stream...and can be passed by value or reference [2]. 2.3 Graphical Processing Units Programming with graphical processing unit ( GPU ) requires a different

  5. 12th International Conference on Computer Graphics Theory and Applications

    CERN Document Server

    2017-01-01

    The International Conference on Computer Graphics Theory and Applications aims at becoming a major point of contact between researchers, engineers and practitioners in Computer Graphics. The conference will be structured along five main tracks, covering different aspects related to Computer Graphics, from Modelling to Rendering, including Animation, Interactive Environments and Social Agents In Computer Graphics.

  6. Engineering Design Graphics: Into the 21st Century

    Science.gov (United States)

    Harris, La Verne Abe; Meyers, Frederick

    2007-01-01

    Graphical plans for construction of machinery and architecture have evolved over the last 6,000 years beginning from hieroglyphics to drawings on printable media, from the "Golden Age" of engineering graphics to the innovation of computer graphics and prototyping. The evolution of engineering design graphics as a profession has also evolved. Years…

  7. A Framework for Mathematics Graphical Tasks: The Influence of the Graphic Element on Student Sense Making

    Science.gov (United States)

    Lowrie, Tom; Diezmann, Carmel M.; Logan, Tracy

    2012-01-01

    Graphical tasks have become a prominent aspect of mathematics assessment. From a conceptual stance, the purpose of this study was to better understand the composition of graphical tasks commonly used to assess students' mathematics understandings. Through an iterative design, the investigation described the sense making of 11-12-year-olds as they…

  8. The effects of repeated exposure to graphic fear appeals on cigarette packages: A field experiment.

    Science.gov (United States)

    Dijkstra, Arie; Bos, Colin

    2015-03-01

    Experimental studies on the effects of graphic fear appeals on cigarette packages typically expose smokers in a single session to a fear appeal, although in practice the exposure is always repeated. The present study applied an improved study design with repeated exposure to fear appeals on cigarette packages. In this field-experiment, 118 smokers were assigned to 1 of 2 conditions with either graphic fear appeals or textual warnings on their cigarette packages. During 3 weeks, fear and disgust were assessed 6 times. The intention to quit smoking after 3 weeks and quitting activity during the 3 weeks were the dependent measures. The effects of 3 pretest individual difference moderators were tested: disengagement beliefs, number of cigarettes smoked a day, and readiness to quit. Three weeks of exposure to the graphic fear appeals led to a stronger intention to quit, but only when smokers scored low on disengagement beliefs, or were heavier smokers. In addition, smokers low in disengagement more often reported to have cut down on smoking in the graphic condition. There were no indications of habituation of fear and disgust over the 3 weeks. The effects of graphic fear appeals depended on smokers' characteristics: The moderators may explain the mixed findings in the literature. The lack of habituation may be caused by the renewal of the graphics every few days. The used field-experimental design with natural repeated exposure to graphics is promising.

  9. Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of black (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles.

    Science.gov (United States)

    Beara, Ivana N; Lesjak, Marija M; Cetojević-Simin, Dragana D; Marjanović, Zaklina S; Ristić, Jelena D; Mrkonjić, Zorica O; Mimica-Dukić, Neda M

    2014-12-15

    The aim of this study was a comprehensive investigation on phenolic profile, antioxidant, anti-inflammatory and cytotoxic activity of the still insufficiently explored black summer truffles (Tuber aestivum Vittad.) and white (Tuber magnatum Pico) truffles. Methanol and water extracts, obtained by maceration and Soxhlet extraction, were examined. Forty-five phenolics was studied using LC-MS/MS: presence of 14 compounds was confirmed, with the most dominant being p-hydroxybenzoic acid, baicalein and kaempferol (T. aestivum), epicatechin and catechin (T. magnatum). Moderate antioxidant activity of both species was determined through several assays. Only T. magnatum showed anti-inflammatory potential by inhibiting COX-1 and 12-LOX pathway products synthesis. Methanol extracts exerted cytotoxicity against some tumour cell lines (HeLa, MCF7, HT-29), besides the prominent activity of water extracts towards breast adenocarcinoma (MCF7). To conclude, these results support further investigations of phytochemicals and biological activity towards verification of nutraceutical use of both species.

  10. Phenylethanoid Glycoside Profiles and Antioxidant Activities of Osmanthus fragrans Lour. Flowers by UPLC/PDA/MS and Simulated Digestion Model.

    Science.gov (United States)

    Jiang, Yirong; Mao, Shuqin; Huang, Weisu; Lu, Baiyi; Cai, Zengxuan; Zhou, Fei; Li, Maiquan; Lou, Tiantian; Zhao, Yajing

    2016-03-30

    Variations of phenylethanoid glycoside profiles and antioxidant activities in Osmanthus fragrans flowers through the digestive tract were evaluated by a simulated digestion model and UPLC/PDA/MS. Major phenylethanoid glycosides and phenolic acids, namely, salidroside, acteoside, isoacteoside, chlorogenic acid, and caffeic acid, were identified in four cultivars of O. fragrans flowers, and the concentration of acteoside was the highest, being up to 71.79 mg/g dry weight. After simulated digestion, total phenylethanoid glycoside contents and antioxidant activities were significantly decreased. Acteoside was identified as decomposing into caffeic acid, whereas salidroside was found to be stable during simulated digestion. According to Pearson's correlation analysis, acteoside contents showed good correlations with antioxidant activities during simulated digestion (R(2) = 0.994, P < 0.01). In conclusion, acteoside was the major contributor to the antioxidant activity of O. fragrans flowers, and salidroside was considered as the major antioxidant compound of O. fragrans flowers in vivo.

  11. Activity-based protein profiling of hydrolytic enzymes induced by gibberellic acid in isolated aleurone layers of malting barley.

    Science.gov (United States)

    Daneri-Castro, Sergio N; Chandrasekar, Balakumaran; Grosse-Holz, Friederike M; van der Hoorn, Renier A L; Roberts, Thomas H

    2016-09-01

    During barley germination, the aleurone layer secretes most of the enzymes required to degrade the endosperm, many of which are yet to be characterized. We used activity-based protein profiling (ABPP) to detect a range of active enzymes extracted from aleurone layers isolated from grains of a commercial malting barley variety incubated with or without gibberellic acid (GA). Enzymes found to be induced by GA were putative aleurains, cathepsin-B-like proteases and serine hydrolases. By using an inhibitory sugar panel, a specific active retaining β-glycosidase in the barley aleurone was identified as a putative xylanase. Our results show that ABPP can be used rapidly to identify a variety of active enzyme isoforms in cereal aleurone without the need for enzyme purification.

  12. Fluorescently activated cell sorting followed by microarray profiling of helper T cell subtypes from human peripheral blood.

    Directory of Open Access Journals (Sweden)

    Chiaki Ono

    Full Text Available BACKGROUND: Peripheral blood samples have been subjected to comprehensive gene expression profiling to identify biomarkers for a wide range of diseases. However, blood samples include red blood cells, white blood cells, and platelets. White blood cells comprise polymorphonuclear leukocytes, monocytes, and various types of lymphocytes. Blood is not distinguishable, irrespective of whether the expression profiles reflect alterations in (a gene expression patterns in each cell type or (b the proportion of cell types in blood. CD4+ Th cells are classified into two functionally distinct subclasses, namely Th1 and Th2 cells, on the basis of the unique characteristics of their secreted cytokines and their roles in the immune system. Th1 and Th2 cells play an important role not only in the pathogenesis of human inflammatory, allergic, and autoimmune diseases, but also in diseases that are not considered to be immune or inflammatory disorders. However, analyses of minor cellular components such as CD4+ cell subpopulations have not been performed, partly because of the limited number of these cells in collected samples. METHODOLOGY/PRINCIPAL FINDINGS: We describe fluorescently activated cell sorting followed by microarray (FACS-array technology as a useful experimental strategy for characterizing the expression profiles of specific immune cells in the circulation. We performed reproducible gene expression profiling of Th1 and Th2, respectively. Our data suggest that this procedure provides reliable information on the gene expression profiles of certain small immune cell populations. Moreover, our data suggest that GZMK, GZMH, EOMES, IGFBP3, and STOM may be novel markers for distinguishing Th1 cells from Th2 cells, whereas IL17RB and CNTNAP1 can be Th2-specific markers. CONCLUSIONS/SIGNIFICANCE: Our approach may help in identifying aberrations and novel therapeutic or diagnostic targets for diseases that affect Th1 or Th2 responses and elucidating the

  13. A Profile of Professional Activities and Practice Patterns for Marriage and Family Therapists in Utah

    OpenAIRE

    1998-01-01

    This research project presents data on practitioner profiles and practice patterns for marriage and family therapists living in Utah . A sample of 77 clinical members and six associate members of the American Association for Marriage and ramily Therapy living in Utah gave descriptive facts on their demographics , training , years of experience , and specific information about their practice of marriage and family therapy. The findings indicate tha t marriage and family therapists in Utah are ...

  14. Enhancements to IRAF/STSDAS graphics

    Science.gov (United States)

    Eisenhamer, J. D.; Levay, Z. G.

    1992-01-01

    The IRAF graphics kernel, psikern, is a true encapsulated PostScript implementation, an improvement over the former SGI-based PostScript output available from IRAF. The psikern kernel implements many more capabilities of gio/gki such as cell arrays (grayscale images), color, filled area patterns and true PostScript fonts. Several of the general-purpose graphics tasks in STSDAS such as igi, sgraph, skymap, and wcslab have been modified to use these capabilities explicitly. Other graphics tasks not enhanced explicitly can also make use of new capabilities such as PostScript font support. We present an overview of psikern and several examples of output created by the enhanced STSDAS tasks.

  15. Item Screening in Graphical Loglinear Rasch Models

    DEFF Research Database (Denmark)

    Kreiner, Svend; Christensen, Karl Bang

    2011-01-01

    the initial item analysis may disclose a great deal of spurious and misleading evidence of DIF and local dependence that has to disposed of during the modelling procedure. Like graphical models, graphical loglinear Rasch models possess Markov properties that are useful during the statistical analysis......In behavioural sciences, local dependence and DIF are common, and purification procedures that eliminate items with these weaknesses often result in short scales with poor reliability. Graphical loglinear Rasch models (Kreiner & Christensen, in Statistical Methods for Quality of Life Studies, ed....... by M. Mesbah, F.C. Cole & M.T. Lee, Kluwer Academic, pp. 187–203, 2002) where uniform DIF and uniform local dependence are permitted solve this dilemma by modelling the local dependence and DIF. Identifying loglinear Rasch models by a stepwise model search is often very time consuming, since...

  16. Data Sorting Using Graphics Processing Units

    Directory of Open Access Journals (Sweden)

    M. J. Mišić

    2012-06-01

    Full Text Available Graphics processing units (GPUs have been increasingly used for general-purpose computation in recent years. The GPU accelerated applications are found in both scientific and commercial domains. Sorting is considered as one of the very important operations in many applications, so its efficient implementation is essential for the overall application performance. This paper represents an effort to analyze and evaluate the implementations of the representative sorting algorithms on the graphics processing units. Three sorting algorithms (Quicksort, Merge sort, and Radix sort were evaluated on the Compute Unified Device Architecture (CUDA platform that is used to execute applications on NVIDIA graphics processing units. Algorithms were tested and evaluated using an automated test environment with input datasets of different characteristics. Finally, the results of this analysis are briefly discussed.

  17. Dynamic Load Balancing using Graphics Processors

    Directory of Open Access Journals (Sweden)

    R Mohan

    2014-04-01

    Full Text Available To get maximum performance on the many-core graphics processors, it is important to have an even balance of the workload so that all processing units contribute equally to the task at hand. This can be hard to achieve when the cost of a task is not known beforehand and when new sub-tasks are created dynamically during execution. Both the dynamic load balancing methods using Static task assignment and work stealing using deques are compared to see which one is more suited to the highly parallel world of graphics processors. They have been evaluated on the task of simulating a computer move against the human move, in the famous four in a row game. The experiments showed that synchronization can be very expensive, and those new methods which use graphics processor features wisely might be required.

  18. Graphic medicine: comics as medical narrative.

    Science.gov (United States)

    Williams, Ian C M

    2012-06-01

    Among the growing number of works of graphic fiction, a number of titles dealing directly with the patient experience of illness or caring for others with an illness are to be found. Thanks in part to the Medical Humanities movement, many medical schools now encourage the reading of classic literature to gain insight into the human condition. Until recently, the medium of comics (the term is used in the plural to refer to both the physical objects and the attendant philosophy and practice surrounding them) has received little attention from healthcare scholars, even though some authors argue that graphic fiction is, in fact, a form of literature. This paper suggests that it is time that the medium was examined by healthcare professionals and studies some acclaimed comic works. Drawing on the principles of narrative medicine, this paper will ask whether comics and graphic novels could be used as a resource for health professionals, patients and carers.

  19. Practical Implementation of a Graphics Turing Test

    DEFF Research Database (Denmark)

    Borg, Mathias; Johansen, Stine Schmieg; Thomsen, Dennis Lundgaard;

    2012-01-01

    graphics. Based on the results from these experiments, future versions of the Graphics Turing Test could ease the restrictions currently necessary in order to test object telepresence under more general conditions. Furthermore, the test could be used to determine the minimum requirements to achieve object......We present a practical implementation of a variation of the Turing Test for realistic computer graphics. The test determines whether virtual representations of objects appear as real as genuine objects. Two experiments were conducted wherein a real object and a similar virtual object is presented...... to test subjects under specific restrictions. A criterion for passing the test is presented based on the probability for the subjects to be unable to recognise a computer generated object as virtual. The experiments show that the specific setup can be used to determine the quality of virtual reality...

  20. Design considerations for parallel graphics libraries

    Science.gov (United States)

    Crockett, Thomas W.

    1994-01-01

    Applications which run on parallel supercomputers are often characterized by massive datasets. Converting these vast collections of numbers to visual form has proven to be a powerful aid to comprehension. For a variety of reasons, it may be desirable to provide this visual feedback at runtime. One way to accomplish this is to exploit the available parallelism to perform graphics operations in place. In order to do this, we need appropriate parallel rendering algorithms and library interfaces. This paper provides a tutorial introduction to some of the issues which arise in designing parallel graphics libraries and their underlying rendering algorithms. The focus is on polygon rendering for distributed memory message-passing systems. We illustrate our discussion with examples from PGL, a parallel graphics library which has been developed on the Intel family of parallel systems.

  1. Graphical Model Theory for Wireless Sensor Networks

    Energy Technology Data Exchange (ETDEWEB)

    Davis, William B.

    2002-12-08

    Information processing in sensor networks, with many small processors, demands a theory of computation that allows the minimization of processing effort, and the distribution of this effort throughout the network. Graphical model theory provides a probabilistic theory of computation that explicitly addresses complexity and decentralization for optimizing network computation. The junction tree algorithm, for decentralized inference on graphical probability models, can be instantiated in a variety of applications useful for wireless sensor networks, including: sensor validation and fusion; data compression and channel coding; expert systems, with decentralized data structures, and efficient local queries; pattern classification, and machine learning. Graphical models for these applications are sketched, and a model of dynamic sensor validation and fusion is presented in more depth, to illustrate the junction tree algorithm.

  2. Transcriptional profiling of the dose response: a more powerful approach for characterizing drug activities.

    Directory of Open Access Journals (Sweden)

    Rui-Ru Ji

    2009-09-01

    Full Text Available The dose response curve is the gold standard for measuring the effect of a drug treatment, but is rarely used in genomic scale transcriptional profiling due to perceived obstacles of cost and analysis. One barrier to examining transcriptional dose responses is that existing methods for microarray data analysis can identify patterns, but provide no quantitative pharmacological information. We developed analytical methods that identify transcripts responsive to dose, calculate classical pharmacological parameters such as the EC50, and enable an in-depth analysis of coordinated dose-dependent treatment effects. The approach was applied to a transcriptional profiling study that evaluated four kinase inhibitors (imatinib, nilotinib, dasatinib and PD0325901 across a six-logarithm dose range, using 12 arrays per compound. The transcript responses proved a powerful means to characterize and compare the compounds: the distribution of EC50 values for the transcriptome was linked to specific targets, dose-dependent effects on cellular processes were identified using automated pathway analysis, and a connection was seen between EC50s in standard cellular assays and transcriptional EC50s. Our approach greatly enriches the information that can be obtained from standard transcriptional profiling technology. Moreover, these methods are automated, robust to non-optimized assays, and could be applied to other sources of quantitative data.

  3. Neon and [CII] 158 micron Emission Line Profiles in Dusty Starbursts and Active Galactic Nuclei

    CERN Document Server

    Samsonyan, Anahit; Lebouteiller, Vianney; Barry, Donald; Sargsyan, Lusine

    2016-01-01

    The sample of 379 extragalactic sources is presented that have mid-infrared, high resolution spectroscopy with the Spitzer Infrared Spectrograph (IRS) and also spectroscopy of the [CII] 158 um line with the Herschel Photodetector Array Camera and Spectrometer (PACS). The emission line profiles of [NeII] 12.81 um, [NeIII] 15.55 um, and [CII] 158 um are presented, and intrinsic line widths are determined (full width half maximum of Gaussian profiles after instrumental correction). All line profiles together with overlays comparing positions of PACS and IRS observations are made available in the Cornell Atlas of Spitzer IRS Sources (CASSIS). Sources are classified from AGN to starburst based on equivalent widths of the 6.2 um polycyclic aromatic hydrocarbon feature. It is found that intrinsic line widths do not change among classification for [CII], with median widths of 207 km per s for AGN, 248 km per s for composites, and 233 km per s for starbursts. The [NeII] line widths also do not change with classificati...

  4. Optimization of HS-GC-FID-MS Method for Residual Solvent Profiling in Active Pharmaceutical Ingredients Using DoE.

    Science.gov (United States)

    Poceva Panovska, Ana; Acevska, Jelena; Stefkov, Gjoshe; Brezovska, Katerina; Petkovska, Rumenka; Dimitrovska, Aneta

    2016-02-01

    Within this research, a headspace (HS) gas chromatography-flame ionization detector-mass spectrometry method was developed for profiling of residual solvents (RSs) in active pharmaceutical ingredients (APIs). Design of experiment was used for optimization of sample preparation, as well as for robustness testing of the method. HS equilibration temperature and dilution medium were detected as parameters with greater impact on the sensitivity, compared with the time used for equilibration of the samples. Regardless of the sample solubility, the use of water for sample preparation was found to be crucial for better sensitivity. The use of a well-designed strategy for method development and robustness testing, additional level of identification confidence, as well as use of internal standard provided a strong and reliable analytical tool for API fingerprinting, thus enabling the authentication of the substance based on the RS profile.

  5. Probabilistic graphical model representation in phylogenetics.

    Science.gov (United States)

    Höhna, Sebastian; Heath, Tracy A; Boussau, Bastien; Landis, Michael J; Ronquist, Fredrik; Huelsenbeck, John P

    2014-09-01

    Recent years have seen a rapid expansion of the model space explored in statistical phylogenetics, emphasizing the need for new approaches to statistical model representation and software development. Clear communication and representation of the chosen model is crucial for: (i) reproducibility of an analysis, (ii) model development, and (iii) software design. Moreover, a unified, clear and understandable framework for model representation lowers the barrier for beginners and nonspecialists to grasp complex phylogenetic models, including their assumptions and parameter/variable dependencies. Graphical modeling is a unifying framework that has gained in popularity in the statistical literature in recent years. The core idea is to break complex models into conditionally independent distributions. The strength lies in the comprehensibility, flexibility, and adaptability of this formalism, and the large body of computational work based on it. Graphical models are well-suited to teach statistical models, to facilitate communication among phylogeneticists and in the development of generic software for simulation and statistical inference. Here, we provide an introduction to graphical models for phylogeneticists and extend the standard graphical model representation to the realm of phylogenetics. We introduce a new graphical model component, tree plates, to capture the changing structure of the subgraph corresponding to a phylogenetic tree. We describe a range of phylogenetic models using the graphical model framework and introduce modules to simplify the representation of standard components in large and complex models. Phylogenetic model graphs can be readily used in simulation, maximum likelihood inference, and Bayesian inference using, for example, Metropolis-Hastings or Gibbs sampling of the posterior distribution.

  6. Graphic representations: keys to disclose the codex of nature

    Science.gov (United States)

    Caramelo, Liliana; Gonçalves, Norberto; Pereira, Mário; Soares, Armando; Naia, Marco

    2010-05-01

    Undergraduate and university level students present some difficulties to understand and interpret many of the geosciences concepts, in particular those represented by vector and scalar fields. Our experience reveals that these difficulties are associated with a lack in the development of their abstraction and mental picturing abilities. On the other hand, these students have easy access to communication and information technology software which can be used to built graphic representations of experimental data, time series and vector and scalar fields. This transformation allows an easiest extraction, interpretation and summary of the most important characteristics in the data. There is already commercial and open source software with graphical tools that can be used for this purpose but commercial software packs with user friendly interfaces but their price is not negligible. Open source software can circumvent this difficulty even if, in general, their graphical user interface hasn't reached the desirable level of the commercial ones. We will show a simple procedure to generate an image from the data that characterizes the generation of the suitable images illustrating the key concepts in study, using a freeware code, exactly as it is presented to the students in our open teaching sessions to the general student community. Our experience demonstrated that the students are very enthusiastic using this approach. Furthermore, the use of this software can easily be adopted by teachers and students of secondary schools as part of curricular activities.

  7. Making and using shaders in modern computer graphics

    OpenAIRE

    2012-01-01

    In the area of consumer computer graphic was a major turning point in the development of graphics and it's rendering, when new graphic cards with programmable pipeline emerged. What and how something will be done in a single stage of programmable pipeline is provided with a small program called shader. The aim of the thesis is to present the basics of modern computer graphics and use of shaders in computer graphics. We start by presenting the theoretical basis of shaders and comparing program...

  8. RNA profiles of porcine embryos during genome activation reveal complex metabolic switch sensitive to in vitro conditions.

    Directory of Open Access Journals (Sweden)

    Olga Østrup

    Full Text Available Fertilization is followed by complex changes in cytoplasmic composition and extensive chromatin reprogramming which results in the abundant activation of totipotent embryonic genome at embryonic genome activation (EGA. While chromatin reprogramming has been widely studied in several species, only a handful of reports characterize changing transcriptome profiles and resulting metabolic changes in cleavage stage embryos. The aims of the current study were to investigate RNA profiles of in vivo developed (ivv and in vitro produced (ivt porcine embryos before (2-cell stage and after (late 4-cell stage EGA and determine major metabolic changes that regulate totipotency. The period before EGA was dominated by transcripts responsible for cell cycle regulation, mitosis, RNA translation and processing (including ribosomal machinery, protein catabolism, and chromatin remodelling. Following EGA an increase in the abundance of transcripts involved in transcription, translation, DNA metabolism, histone and chromatin modification, as well as protein catabolism was detected. The further analysis of members of overlapping GO terms revealed that despite that comparable cellular processes are taking place before and after EGA (RNA splicing, protein catabolism, different metabolic pathways are involved. This strongly suggests that a complex metabolic switch accompanies EGA. In vitro conditions significantly altered RNA profiles before EGA, and the character of these changes indicates that they originate from oocyte and are imposed either before oocyte aspiration or during in vitro maturation. IVT embryos have altered content of apoptotic factors, cell cycle regulation factors and spindle components, and transcription factors, which all may contribute to reduced developmental competence of embryos produced in vitro. Overall, our data are in good accordance with previously published, genome-wide profiling data in other species. Moreover, comparison with mouse and

  9. Changes in growth, photosynthetic activities, biochemical parameters and amino acid profile of Thompson Seedless grapes (Vitis vinifera L.).

    Science.gov (United States)

    Somkuwar, R G; Bahetwar, Anita; Khan, I; Satisha, J; Ramteke, S D; Itroutwar, Prerna; Bhongale, Aarti; Oulkar, Dashrath

    2014-11-01

    The study on photosynthetic activity and biochemical parameters in Thompson Seedless grapes grafted on Dog Ridge rootstock and its impact on growth, yield and amino acid profile at various stages of berry development was conducted during the year 2012-2013. Leaf and berry samples from ten year old vines of Thompson Seedless were collected at different growth and berry developmental stages. The analysis showed difference in photosynthetic activity, biochemical parameters and amino acid status with the changes in berry development stage. Higher photosynthetic rate of 17.39 umol cm(-2) s(-1) was recorded during 3-4mm berry size and the lowest (10.08 umol cm(-2) s(-1)) was recorded during the veraison stage. The photosynthetic activity showed gradual decrease with the onset of harvest while the different biochemical parameters showed increase and decrease from one stage to another in both berry and leaves. Changes in photosynthetic activity and biochemical parameters thereby affected the growth, yield and amino acid content of the berry. Positive correlation of leaf area and photosynthetic rate was recorded during the period of study. Reducing sugar (352.25 mg g(-1)) and total carbohydrate (132.52 mg g(-1)) was more in berries as compared to leaf. Amino acid profile showed variations in different stages of berry development. Marked variations in photosynthetic as well as biochemical and amino acid content at various berry development stages was recorded and thereby its cumulative effect on the development of fruit quality.

  10. Graphicality transitions in scale-free networks

    CERN Document Server

    Del Genio, Charo I; Bassler, Kevin E

    2011-01-01

    We study the graphicality of power-law distributed degree sequences, showing that the fraction of graphical sequences undergoes two sharp transitions at the values 0 and 2 of the power-law exponent. We characterize these transitions as first-order, and provide an analytic explanation of their nature. Further numerical calculations, based on extreme value arguments, verify this treatment, and introduce a method to determine transition points for any given degree distribution. Our results reveal a fundamental reason why scale-free networks with no constraints on minimum and maximum degree must be sparse for positive power-law exponents, and dense otherwise.

  11. Object-oriented graphics programming in C++

    CERN Document Server

    Stevens, Roger T

    2014-01-01

    Object-Oriented Graphics Programming in C++ provides programmers with the information needed to produce realistic pictures on a PC monitor screen.The book is comprised of 20 chapters that discuss the aspects of graphics programming in C++. The book starts with a short introduction discussing the purpose of the book. It also includes the basic concepts of programming in C++ and the basic hardware requirement. Subsequent chapters cover related topics in C++ programming such as the various display modes; displaying TGA files, and the vector class. The text also tackles subjects on the processing

  12. Imagined Spaces: Motion Graphics in Performance Spaces

    DEFF Research Database (Denmark)

    Steijn, Arthur

    2016-01-01

    In this chapter I introduce the first steps in my work with adjoining and developing concepts relevant to the study and practical design of motion graphics in spatial experience design; performance, event and exhibition design. Based on a presentation of a practical case where motion graphics...... through theories drawn from two different fields. The first is from the field of direct visual perception as explored and described by the American psychologist J. J. Gibson. I supplement this angle by introducing relevant new media theories extracted from writings from L. Manovich. I also briefly...

  13. Building probabilistic graphical models with Python

    CERN Document Server

    Karkera, Kiran R

    2014-01-01

    This is a short, practical guide that allows data scientists to understand the concepts of Graphical models and enables them to try them out using small Python code snippets, without being too mathematically complicated. If you are a data scientist who knows about machine learning and want to enhance your knowledge of graphical models, such as Bayes network, in order to use them to solve real-world problems using Python libraries, this book is for you. This book is intended for those who have some Python and machine learning experience, or are exploring the machine learning field.

  14. Vertical profiles of dioxin-like and estrogenic activities associated with a sediment core from Tokyo Bay, Japan

    Energy Technology Data Exchange (ETDEWEB)

    Kannan, K.; Villeneuve, D.L.; Yamashita, N.; Imagawa, T.; Hashimoto, S.; Miyazaki, A.; Giesy, J.P. [Michigan State University, East Lansing, MI (USA). Dept. of Zoology

    2000-09-01

    In vitro bioassays were used to measure dioxin-like and estrogenic activities associated with florisil fractions of extracts from a sediment core collected from Tokyo Bay, Japan. Florisil fractions 2(F2) and 3(F3) elicited significant dioxin-like responses in vitro. Dioxin-like activity of F2 samples were correlated with the vertical profile of PAH concentrations (R{sup 2} = 0.85). Contribution of PAHs to Ah receptor-mediated activities in sediments was greater than those by PCDDs/DFs, PCBs, and PCNs. The dioxin-like activity of F3 samples suggests the presence of relatively polar, Ah receptor-active compounds in the Tokyo Bay sediment core. Significant estrogenic activities, which may be related to the presence of certain estrogenic PAHs, were observed for F2 samples. Estrogen equivalents (E2-EQs) calculated from the concentrations and relative potencies of known estrogenic compounds in F2 were greater than bioassay-derived E2-EQs. This suggests that complex interactions between estrogenic and antiestrogenic compounds (PAHs, PCDD/DFs, and PCNs) may have modulated the activity. F3 samples were toxic to MVLN cells; therefore, their estrogenic activities could not be estimated. 27 refs., 4 figs., 3 tabs.

  15. Anomalous Flattening of the Fast-Ion Profile during Alfvén-Eigenmode Activity

    Science.gov (United States)

    Heidbrink, W. W.; Gorelenkov, N. N.; Luo, Y.; van Zeeland, M. A.; White, R. B.; Austin, M. E.; Burrell, K. H.; Kramer, G. J.; Makowski, M. A.; McKee, G. R.; Nazikian, R.

    2007-12-01

    Neutral-beam injection into plasmas with negative central shear produces a rich spectrum of toroidicity-induced and reversed-shear Alfvén eigenmodes in the DIII-D tokamak. The first application of fast-ion Dα (FIDA) spectroscopy to Alfvén-eigenmode physics shows that the central fast-ion profile is anomalously flat in the inner half of the discharge. Neutron and equilibrium measurements corroborate the FIDA data. The current density driven by fast ions is also strongly modified. Calculations based on the measured mode amplitudes do not explain the observed fast-ion transport.

  16. Soccer activity profile of altitude versus sea-level natives during acclimatisation to 3600 m (ISA3600)

    Science.gov (United States)

    Aughey, Robert J; Hammond, Kristal; Varley, Matthew C; Schmidt, Walter F; Bourdon, Pitre C; Buchheit, Martin; Simpson, Ben; Garvican-Lewis, Laura A; Kley, Marlen; Soria, Rudy; Sargent, Charli; Roach, Gregory D; Claros, Jesus C Jimenez; Wachsmuth, Nadine; Gore, Christopher J

    2013-01-01

    Objectives We investigated the effect of high altitude on the match activity profile of elite youth high altitude and sea level residents. Methods Twenty Sea Level (Australian) and 19 Altitude-resident (Bolivian) soccer players played five games, two near sea level (430 m) and three in La Paz (3600 m). Match activity profile was quantified via global positioning system with the peak 5 min period for distance ((D5peak)) and high velocity running (>4.17 m/s, HIVR5peak); as well as the 5 min period immediately subsequent to the peak for both distance (D5sub) and high-velocity running (HIVR5sub) identified using a rolling 5 min epoch. The games at 3600 m were compared with the average of the two near sea-level games. Results The total distance per minute was reduced by a small magnitude in the first match at altitude in both teams, without any change in low-velocity running. There were variable changes in HiVR, D5peak and HiVR5peak from match to match for each team. There were within-team reductions in D5peak in each game at altitude compared with those at near sea level, and this reduction was greater by a small magnitude in Australians than Bolivians in game 4. The effect of altitude on HiVR5peak was moderately lower in Australians compared with Bolivians in game 3. There was no clear difference in the effect of altitude on maximal accelerations between teams. Conclusions High altitude reduces the distance covered by elite youth soccer players during matches. Neither 13 days of acclimatisation nor lifelong residence at high altitude protects against detrimental effects of altitude on match activity profile. PMID:24282196

  17. Theoretical Emission-Line Profiles of Active Galactic Nuclei and the Unified Model. I. The Face-on Torus

    Science.gov (United States)

    Quintilio, R.; Viegas, S. M.

    1997-01-01

    Theoretical emission-line profiles are obtained for active galactic nuclei (AGNs) taking into account the presence of an obscuring torus around the central energy source. For the sake of simplicity, the torus is represented by a cylindrical shell characterized by the inner and outer radius and the opening angle. In this paper we discuss the results with angle of sight equal to 0, i.e., for a face-on torus. Different line profiles are obtained following the torus parameters. The line profiles may show more than one peak and bumps, depending on the torus dimensions. The main parameter determining the number of peaks or bumps is the opening angle. Thus, the observed line shape may be a good indicator of the torus characteristics. As an example, the fit to the observed [O III] λ5007 emission line of NGC 4151 is presented. The model reproduces the FWHM and the asymmetrical bumps observed. Partially supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) under Grant 92/4335-9.

  18. Altered circadian blood pressure profile in patients with active acromegaly. Relationship with left ventricular mass and hormonal values.

    Science.gov (United States)

    Pietrobelli, D J; Akopian, M; Olivieri, A O; Renauld, A; Garrido, D; Artese, R; Feldstein, C A

    2001-09-01

    To determine the relationships between the circadian blood pressure profile and left ventricular mass, hormonal pattern and insulin sensitivity indices in patients with active acromegaly, ambulatory 24-h blood pressure monitoring (ABPM) was recorded in 25 subjects (47.0 +/- 15.1 years, range 23-72). Serum growth hormone (GH) and insulin-like growth factor-1, fasting and mean plasma glucose and insulin during oral glucose tolerance test (OGTT), insulinogenic index, the sum of the plasma insulin levels and the homeostasis model insulin resistance index (Homa's index) were determined. Left ventricular mass index (LVMI) was calculated from two-dimensional guided M-mode echocardiogram. The prevalence of hypertension was 56% (n = 14) and 40% (n = 10) according to sphygmomanometric measurements and ABPM, respectively. Non-dipping profile was observed in six of 10 hypertensives and in six of 15 normotensives. Serum growth hormone, fasting glucose, the area under the serum insulin curve and LVMI were higher for acromegalics with non-dipping profile than for dippers (all of them, P regulation in essential hypertension deserves further studies.

  19. Superplot: Graphical interface for plotting and analyzing data

    Science.gov (United States)

    Fowlie, Andrew; Bardsley, Michael Hugh

    2016-12-01

    Superplot calculates and plots statistical quantities relevant to parameter inference from a "chain" of samples drawn from a parameter space produced by codes such as MultiNest (ascl:1109.006), BAYES-X (ascl:1505.027), and PolyChord (ascl:1502.011). It offers a graphical interface for browsing a chain of many variables quickly and can produce numerous kinds of publication quality plots, including one- and two-dimensional profile likelihood, three-dimensional scatter plots, and confidence intervals and credible regions. Superplot can also save plots in PDF format, create a summary text file, and export a plot as a pickled object for importing and manipulating in a Python interpreter.

  20. Profiling of Concanavalin A-Binding Glycoproteins in Human Hepatic Stellate Cells Activated with Transforming Growth Factor-β1

    Directory of Open Access Journals (Sweden)

    Yannan Qin

    2014-11-01

    Full Text Available Glycoproteins play important roles in maintaining normal cell functions depending on their glycosylations. Our previous study indicated that the abundance of glycoproteins recognized by concanavalin A (ConA was increased in human hepatic stellate cells (HSCs following activation by transforming growth factor-β1 (TGF-β1; however, little is known about the ConA-binding glycoproteins (CBGs of HSCs. In this study, we employed a targeted glycoproteomics approach using lectin-magnetic particle conjugate-based liquid chromatography-tandem mass spectrometry to compare CBG profiles between LX-2 HSCs with and without activation by TGF-β1, with the aim of discovering novel CBGs and determining their possible roles in activated HSCs. A total of 54 and 77 proteins were identified in the quiescent and activated LX-2 cells, respectively. Of the proteins identified, 14.3% were glycoproteins and 73.3% were novel potential glycoproteins. Molecules involved in protein processing in the endoplasmic reticulum (e.g., calreticulin and calcium signaling (e.g., 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase β-2 [PLCB2] were specifically identified in activated LX-2 cells. Additionally, PLCB2 expression was upregulated in the cytoplasm of the activated LX-2 cells, as well as in the hepatocytes and sinusoidal cells of liver cirrhosis tissues. In conclusion, the results of this study may aid future investigations to find new molecular mechanisms involved in HSC activation and antifibrotic therapeutic targets.

  1. Evaluation of Antioxidant and Antimicrobial Activities and Phenolic Profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys

    Directory of Open Access Journals (Sweden)

    Laurian Vlase

    2014-04-01

    Full Text Available This study was designed to examine the in vitro antioxidant and antimicrobial activities and to characterize the polyphenolic composition of the ethanolic extracts of Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Qualitative and quantitative analysis of the major phenolic compounds were conducted using high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS. The total polyphenols, caffeic acid derivatives and flavonoids content was spectrophotometrically determined. The phenolic profile showed the presence of phenolic acid derivatives (caftaric, gentisic, caffeic, p-coumaric, chlorogenic and ferulic acids, flavonoid glycosides (rutin, isoquercitrin and quercitrin and free flavonoid aglycons (luteolin, quercetin, in different concentrations. DPPH radical scavenging assay, Trolox equivalent antioxidant capacity (TEAC method, hemoglobin ascorbate peroxidase activity inhibition (HAPX assay, and electron paramagnetic resonance (EPR radicals detection were employed, revealing several aspects of the antioxidant activities of these species. The antimicrobial tests were performed using the disk diffusion assay. These extracts contained a large amount of the polyphenolic compounds (77.72, 175.57, and 243.65 mg/g, respectively, and they showed a good antioxidant activity, as witnessed by a number of methods. T. chamaedrys had a high antimicrobial activity. Besides their antioxidant activity, the antimicrobial effect of these extracts confirms the biological activities of these herbal medicinal products.

  2. Evaluation of antioxidant and antimicrobial activities and phenolic profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys.

    Science.gov (United States)

    Vlase, Laurian; Benedec, Daniela; Hanganu, Daniela; Damian, Grigore; Csillag, Ioan; Sevastre, Bogdan; Mot, Augustin C; Silaghi-Dumitrescu, Radu; Tilea, Ioan

    2014-04-28

    This study was designed to examine the in vitro antioxidant and antimicrobial activities and to characterize the polyphenolic composition of the ethanolic extracts of Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Qualitative and quantitative analysis of the major phenolic compounds were conducted using high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). The total polyphenols, caffeic acid derivatives and flavonoids content was spectrophotometrically determined. The phenolic profile showed the presence of phenolic acid derivatives (caftaric, gentisic, caffeic, p-coumaric, chlorogenic and ferulic acids), flavonoid glycosides (rutin, isoquercitrin and quercitrin) and free flavonoid aglycons (luteolin, quercetin), in different concentrations. DPPH radical scavenging assay, Trolox equivalent antioxidant capacity (TEAC) method, hemoglobin ascorbate peroxidase activity inhibition (HAPX) assay, and electron paramagnetic resonance (EPR) radicals detection were employed, revealing several aspects of the antioxidant activities of these species. The antimicrobial tests were performed using the disk diffusion assay. These extracts contained a large amount of the polyphenolic compounds (77.72, 175.57, and 243.65 mg/g, respectively), and they showed a good antioxidant activity, as witnessed by a number of methods. T. chamaedrys had a high antimicrobial activity. Besides their antioxidant activity, the antimicrobial effect of these extracts confirms the biological activities of these herbal medicinal products.

  3. Human response times in a graphic environment

    CERN Document Server

    Yule, A

    1972-01-01

    A summary of the results obtained from measuring the response times of the users of an interactive graphics system available on the CERN central computers is presented. These results are then used to find an optimum time to wait before rolling the user's program to disc.

  4. Fast Gridding on Commodity Graphics Hardware

    DEFF Research Database (Denmark)

    Sørensen, Thomas Sangild; Schaeffter, Tobias; Noe, Karsten Østergaard;

    2007-01-01

    is the far most time consuming of the three steps (Table 1). Modern graphics cards (GPUs) can be utilised as a fast parallel processor provided that algorithms are reformulated in a parallel solution. The purpose of this work is to test the hypothesis, that a non-cartesian reconstruction can be efficiently...

  5. Fast Sparse Level Sets on Graphics Hardware

    NARCIS (Netherlands)

    Jalba, Andrei C.; Laan, Wladimir J. van der; Roerdink, Jos B.T.M.

    2013-01-01

    The level-set method is one of the most popular techniques for capturing and tracking deformable interfaces. Although level sets have demonstrated great potential in visualization and computer graphics applications, such as surface editing and physically based modeling, their use for interactive sim

  6. On Repairing Generated Behaviors for Graphical Characters

    DEFF Research Database (Denmark)

    Corradini, Andrea; Mehta, Manish

    2016-01-01

    In this paper, we continue our work on the creation of artificial intelligence (AI) behaviors for graphical interactive characters by novice users. We refer to novice users as any persons who do not have any particular skills, training and experience in both programming and design. The focus...

  7. Textbook Graphics and Maps: Keys to Learning.

    Science.gov (United States)

    Danzer, Gerald A.

    1980-01-01

    Explains how social studies pupils can use an awareness of textbook design to become better students. Suggestions include reproducing the collage on an American history textbook as a large poster for classroom use and directing students to design a graphic unit opener in the same style as the ones in their textbooks. (DB)

  8. Graphical route information on variable message signs

    NARCIS (Netherlands)

    Alkim, T.P.; Mede, P.H.J. van der; Janssen, W.H.

    2002-01-01

    Reports on experiments in the Netherlands on the use of graphical route information panels (GRIP) as part of variable message systems (VMS) providing information to drivers. GRIP appear to be as safe as regular VMS. Digestion of the information presented is initially quicker for regular VMS, but mot

  9. Application of ggplot2 to Pharmacometric Graphics

    OpenAIRE

    2013-01-01

    Visualization is a powerful mechanism for extracting information from data. ggplot2 is a contributed visualization package in the R programming language, which creates publication-quality statistical graphics in an efficient, elegant, and systematic manner. This article summarizes key features of the package with examples from pharmacometrics and pointers to available resources for learning ggplot2.

  10. Codesign Analysis of a Computer Graphics Application

    DEFF Research Database (Denmark)

    Madsen, Jan; Brage, Jens P.

    1996-01-01

    This paper describes a codesign case study where a computer graphics application is examined with the intention to speed up its execution. The application is specified as a C program, and is characterized by the lack of a simple compute-intensive kernel. The hardware/software partitioning is based...

  11. [Graphic recording of the maxillary ostium permeability].

    Science.gov (United States)

    Rosique, M; Pastor, A; Hellín, D; García-Ortega, F P

    1993-01-01

    Currently we practise salpingography for evaluating Eustachian tube permeability. In a similar mode, we describe a technique of graphic search, with impedianciometry, of patency in the osteomeatal complex, after puncture and placing Foley's catheter in the maxillary sinus. In several cases without opening, we make topical treatment though catheter. For this technique, we propose the term of infundibulography.

  12. Graphic Communications. Progress Record, Theory Outline.

    Science.gov (United States)

    Connecticut State Dept. of Education, Hartford. Div. of Vocational-Technical Schools.

    Intended to reduce unnecessary paper work on the part of the shop instructor in a graphic communications course, this job assignment book offers a simplified method of keeping student records up-to-date. It first provides a record/form with areas for student name, tool check number, locker number, textbook number, and grades; broad course…

  13. Dynamic Stall Analysis Utilizing Interactive Computer Graphics

    Science.gov (United States)

    1988-03-01

    Blade-Vortex Interaction (BV[) studies. solkes the two-dimen i,)nal, unsteady, compressible Euler and Napier -Stokes equations in strong conservation...requirements, interactive computer graphics workstations have been evolved to complement the super -computer. Workstation capabilities, in terms of

  14. Exploring Identity and Multiliteracies through Graphic Narratives

    Science.gov (United States)

    King, Alyson E.

    2015-01-01

    In a first-year, university-level communication course that examined issues of race, ethnicity, postcolonialism, diaspora, and coming-of-age using different points of view and modes of communication, students created graphic novel-style auto-ethnographies to reflect on their experiences with diaspora and identity creation. The assignment was an…

  15. Graphic method for analyzing common path interferometers

    DEFF Research Database (Denmark)

    Glückstad, J.

    1998-01-01

    Common path interferometers are widely used for visualizing phase disturbances and fluid flows. They are attractive because of the inherent simplicity and robustness in the setup. A graphic method will be presented for analyzing and optimizing filter parameters in common path interferometers....

  16. Harvesting graphics power for MD simulations

    NARCIS (Netherlands)

    van Meel, J.A.; Arnold, A.; Frenkel, D.; Portegies Zwart, S.F.; Belleman, R.G.

    2008-01-01

    We discuss an implementation of molecular dynamics (MD) simulations on a graphic processing unit (GPU) in the NVIDIA CUDA language. We tested our code on a modern GPU, the NVIDIA GeForce 8800 GTX. Results for two MD algorithms suitable for short-ranged and long-ranged interactions, and a congruentia

  17. Graphics processing unit-assisted lossless decompression

    Energy Technology Data Exchange (ETDEWEB)

    Loughry, Thomas A.

    2016-04-12

    Systems and methods for decompressing compressed data that has been compressed by way of a lossless compression algorithm are described herein. In a general embodiment, a graphics processing unit (GPU) is programmed to receive compressed data packets and decompress such packets in parallel. The compressed data packets are compressed representations of an image, and the lossless compression algorithm is a Rice compression algorithm.

  18. A Codesign Case Study in Computer Graphics

    DEFF Research Database (Denmark)

    Brage, Jens P.; Madsen, Jan

    1994-01-01

    The paper describes a codesign case study where a computer graphics application is examined with the intention to speed up its execution. The application is specified as a C program, and is characterized by the lack of a simple compute-intensive kernel. The hardware/software partitioning is based...

  19. Graphic Novels in Libraries: An Expert's Opinion

    Science.gov (United States)

    Foster, Katy

    2004-01-01

    Barbara Gordon a librarian and computer expert from Gotham city is a genius level intellect and photographic memory expert at research and analysis. According to her, graphic novels and comics are wildly appealing to readers of all ages and intensely popular with adolescents.

  20. Disjoint forms in graphical user interfaces

    NARCIS (Netherlands)

    Evers, S.; Achten, P.M.; Plasmeijer, M.J.; Loidl, H.W.

    2006-01-01

    Forms are parts of a graphical user interface (GUI) that show a set of values and allow the user to update them. The declarative form construction library FunctionalForms is extended with disjoint form combinators to capture some common patterns in which the form structure expresses a choice. We dem

  1. A Graphical CAL Program for Fourier Series.

    Science.gov (United States)

    McKenzie, J.

    1982-01-01

    A computer program, making use of interactive computer graphics, has been developed to help students become fluent in the mathematical procedures needed to understand concepts of addition of waves. Background theory, use of the program, and technical and educational features of the program (written in Fortran) are discussed. (Author/JN)

  2. Bringing Graphic Novels into a School's Curriculum

    Science.gov (United States)

    Bucher, Katherine T.; Manning, M. Lee

    2004-01-01

    Many young adults enjoy graphic novels because the genre differs so dramatically from the books that educators traditionally have encouraged adolescents to read. Growing up with television and video games, contemporary young adults look for print media that contain the same visual impact and pared-down writing style and contribute to their…

  3. Introduction to 3D Graphics through Excel

    Science.gov (United States)

    Benacka, Jan

    2013-01-01

    The article presents a method of explaining the principles of 3D graphics through making a revolvable and sizable orthographic parallel projection of cuboid in Excel. No programming is used. The method was tried in fourteen 90 minute lessons with 181 participants, which were Informatics teachers, undergraduates of Applied Informatics and gymnasium…

  4. Interactive Learning for Graphic Design Foundations

    Science.gov (United States)

    Chu, Sauman; Ramirez, German Mauricio Mejia

    2012-01-01

    One of the biggest problems for students majoring in pre-graphic design is students' inability to apply their knowledge to different design solutions. The purpose of this study is to examine the effectiveness of interactive learning modules in facilitating knowledge acquisition during the learning process and to create interactive learning modules…

  5. Graphical Method for Determining Projectile Trajectory

    Science.gov (United States)

    Moore, J. C.; Baker, J. C.; Franzel, L.; McMahon, D.; Songer, D.

    2010-01-01

    We present a nontrigonometric graphical method for predicting the trajectory of a projectile when the angle and initial velocity are known. Students enrolled in a general education conceptual physics course typically have weak backgrounds in trigonometry, making inaccessible the standard analytical calculation of projectile range. Furthermore,…

  6. Graphic Resources in the Spanish Art Libraries.

    Science.gov (United States)

    Medina, Alicia Garcia; Coso, Teresa

    The interest in graphic information has increased explosively and is explored through various communication networks like the Internet and the audiovisual media. Art libraries, museums, cultural centers, and art foundations manage and store a lot of images in several departments and in print and digital formats. This paper discusses the cataloging…

  7. A graphics primer for English Teachers

    Science.gov (United States)

    Brillhart, L. V.; Debs, M. B.

    1981-01-01

    Skills necessary for teaching graphics are addressed. A simple, step by step method of teaching students how to draw different types of graphs is presented. Each step is illustrated by a drawing. Some audience analysis for the determination of appropriateness of the use of different types of graphs is included.

  8. Multiple Learning Strategies Project. Graphics. EMI.

    Science.gov (United States)

    Steinberg, Alan; And Others

    This instructional package, designed for educable mentally impaired students, focuses on the vocational area of graphics. Contained in this document are nine learning modules organized into a finishing and bindery unit. Maintenance of a Challenge power cutter, operation of a hand electric stapler, and packaging with kraft paper are examples of…

  9. Circuit II--A Conversational Graphical Interface.

    Science.gov (United States)

    Singer, Ronald A.

    1993-01-01

    Provides an overview of Circuit II, an interactive system that provides users with a graphical representation of an electronic circuit within which questions may be posed and manipulated, and discusses how mouse selections have analogous roles to certain natural language features, such as anaphora, deixis, and ellipsis. (13 references) (EA)

  10. Clustering Finnish Gambler Profiles Based on the Money and Time Consumed in Gambling Activities.

    Science.gov (United States)

    Heiskanen, Maria; Toikka, Arho

    2016-06-01

    Gambling involves consumption of gamblers' money and time. Gamblers are a heterogeneous group, and in addition to grouping gamblers based on personality factors, it is also important to find different gambler profiles with respect to their gambling behavior. Using the nationally representative survey 'Finnish Gambling 2011' (N = 4484), this article studies the subtypes of Finnish gamblers based on the frequency of gambling and the amounts of money and time used in different gambling forms. Cluster analysis reveals six profiles of gamblers, from infrequent gamblers to omnivorous gamblers. In the further analysis of the clusters, it was found that the highest problem gambling prevalence was in the groups of sport betting + electronic gaming machine gamblers and omnivorous gamblers, which were also both dominated by men. Certain gambling consumption patterns and risk factors for problem gambling are related to both socio-demographic backgrounds of the gamblers as well as the structural and situational characteristics of the games. The results have implications for the prevention of problem gambling, as some consumption patterns may be connected with the probability of developing gambling problems.

  11. Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation.

    Directory of Open Access Journals (Sweden)

    Ghulam Jeelani

    Full Text Available Encystation, which is cellular differentiation from the motile, proliferative, labile trophozoite form to the dormant, resistant cyst form, is a crucial process found in parasitic and free-living protozoa such as Entamoeba, Giardia, Acanthamoeba, and Balamuthia. Since encystation is an essential process to deal with the adverse external environmental changes during the life cycle, and often integral to the transmission of the diseases, biochemical understanding of the process potentially provides useful measures against the infections caused by this group of protozoa. In this study, we investigated metabolic and transcriptomic changes that occur during encystation in Entamoeba invadens, the reptilian sibling of mammal-infecting E. histolytica, using capillary electrophoresis-tandem mass spectrometry-based metabolite profiling and DNA microarray-based expression profiling. As the encystation progressed, the levels of majority of metabolites involved in glycolysis and nucleotides drastically decreased, indicating energy generation is ceased. Furthermore, the flux of glycolysis was redirected toward chitin wall biosynthesis. We found remarkable temporal increases in biogenic amines such as isoamylamine, isobutylamine, and cadaverine, during the early period of encystation, when the trophozoites form large multicellular aggregates (precyst. We also found remarkable induction of γ-aminobutyric acid (GABA during encystation. This study has unveiled for the first time the dynamics of the transcriptional and metabolic regulatory networks during encystation, and should help in better understanding of the process in pathogenic eukaryotes, and further development of measures controlling infections they cause.

  12. Cosmological simulations of galaxy clusters with feedback from active galactic nuclei: profiles and scaling relations

    CERN Document Server

    Pike, Simon R; Newton, Richard D A; Thomas, Peter A; Jenkins, Adrian

    2014-01-01

    We present results from a new set of 30 cosmological simulations of galaxy clusters, including the effects of radiative cooling, star formation, supernova feedback, black hole growth and AGN feedback. We first demonstrate that our AGN model is capable of reproducing the observed cluster pressure profile at redshift, z~0, once the AGN heating temperature of the targeted particles is made to scale with the final virial temperature of the halo. This allows the ejected gas to reach larger radii in higher-mass clusters than would be possible had a fixed heating temperature been used. Such a model also successfully reduces the star formation rate in brightest cluster galaxies and broadly reproduces a number of other observational properties at low redshift, including baryon, gas and star fractions; entropy profiles outside the core; and the X-ray luminosity-mass relation. Our results are consistent with the notion that the excess entropy is generated via selective removal of the densest material through radiative c...

  13. Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation.

    Science.gov (United States)

    Jeelani, Ghulam; Sato, Dan; Husain, Afzal; Escueta-de Cadiz, Aleyla; Sugimoto, Masahiro; Soga, Tomoyoshi; Suematsu, Makoto; Nozaki, Tomoyoshi

    2012-01-01

    Encystation, which is cellular differentiation from the motile, proliferative, labile trophozoite form to the dormant, resistant cyst form, is a crucial process found in parasitic and free-living protozoa such as Entamoeba, Giardia, Acanthamoeba, and Balamuthia. Since encystation is an essential process to deal with the adverse external environmental changes during the life cycle, and often integral to the transmission of the diseases, biochemical understanding of the process potentially provides useful measures against the infections caused by this group of protozoa. In this study, we investigated metabolic and transcriptomic changes that occur during encystation in Entamoeba invadens, the reptilian sibling of mammal-infecting E. histolytica, using capillary electrophoresis-tandem mass spectrometry-based metabolite profiling and DNA microarray-based expression profiling. As the encystation progressed, the levels of majority of metabolites involved in glycolysis and nucleotides drastically decreased, indicating energy generation is ceased. Furthermore, the flux of glycolysis was redirected toward chitin wall biosynthesis. We found remarkable temporal increases in biogenic amines such as isoamylamine, isobutylamine, and cadaverine, during the early period of encystation, when the trophozoites form large multicellular aggregates (precyst). We also found remarkable induction of γ-aminobutyric acid (GABA) during encystation. This study has unveiled for the first time the dynamics of the transcriptional and metabolic regulatory networks during encystation, and should help in better understanding of the process in pathogenic eukaryotes, and further development of measures controlling infections they cause.

  14. Effect of game format on heart rate, activity profile, and player involvement in elite and recreational youth players

    DEFF Research Database (Denmark)

    Randers, M B; Andersen, T. Bull; Rasmussen, L S;

    2014-01-01

    The purpose of this study was to evaluate activity profile, aerobic load, and player involvement in two game formats of recreational and elite youth football for two age groups. A total of 152 youth players participated, with 45 U10 players playing 5v5 and 8v8 games, and 41 U13 players playing 8v8...... high in youth football matches irrespective of the level of play and the game format. Playing with fewer players on smaller pitches results...... in minor changes to the physical loading but elevates the technical involvement of youth players both at elite level and recreational level....

  15. A characterization for a graphic sequence to be potentially C_r-graphic

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Let r 3, n r and π = (d1, d2, . . . , dn) be a graphic sequence. If there exists a simple graph G on n vertices having degree sequence π such that G contains Cr (a cycle of length r) as a subgraph, then π is said to be potentially Cr-graphic. Li and Yin (2004) posed the following problem: characterize π = (d1, d2, . . . , dn) such that π is potentially Cr-graphic for r 3 and n r. Rao and Rao (1972) and Kundu (1973) answered this problem for the case of n = r. In this paper, this problem is solved completely.

  16. Risk Management Collaboration through Sharing Interactive Graphics

    Science.gov (United States)

    Slingsby, Aidan; Dykes, Jason; Wood, Jo; Foote, Matthew

    2010-05-01

    Risk management involves the cooperation of scientists, underwriters and actuaries all of whom analyse data to support decision-making. Results are often disseminated through static documents with graphics that convey the message the analyst wishes to communicate. Interactive graphics are increasingly popular means of communicating the results of data analyses because they enable other parties to explore and visually analyse some of the data themselves prior to and during discussion. Discussion around interactive graphics can occur synchronously in face-to-face meetings or with video-conferencing and screen sharing or they can occur asynchronously through web-sites such as ManyEyes, web-based fora, blogs, wikis and email. A limitation of approaches that do not involve screen sharing is the difficulty in sharing the results of insights from interacting with the graphic. Static images accompanied can be shared but these themselves cannot be interacted, producing a discussion bottleneck (Baker, 2008). We address this limitation by allowing the state and configuration of graphics to be shared (rather than static images) so that a user can reproduce someone else's graphic, interact with it and then share the results of this accompanied with some commentary. HiVE (Slingsby et al, 2009) is a compact and intuitive text-based language that has been designed for this purpose. We will describe the vizTweets project (a 9-month project funded by JISC) in which we are applying these principles to insurance risk management in the context of the Willis Research Network, the world's largest collaboration between the insurance industry and the academia). The project aims to extend HiVE to meet the needs of the sector, design, implement free-available web services and tools and to provide case studies. We will present a case study that demonstrate the potential of this approach for collaboration within the Willis Research Network. Baker, D. Towards Transparency in Visualisation Based

  17. Phytochemical profile, antioxidant and cytotoxic activities of the carob tree (Ceratonia siliqua L.) germ flour extracts.

    Science.gov (United States)

    Custódio, Luísa; Escapa, Ana Luísa; Fernandes, Eliana; Fajardo, Alba; Aligué, Rosa; Alberício, Fernando; Neng, Nuno; Nogueira, José Manuel Florêncio; Romano, Anabela

    2011-03-01

    This work aimed to evaluate the phytochemical content and to determine the antioxidant and cytotoxic activities of methanol extracts of the carob tree (Ceratonia siliqua L.) germ flour. The extracts were rich in phenolic compounds, had considerable antioxidant activity, and reduced the viability of cervical (HeLa) cancer cells. The chemical content and the biological activities of the extracts were significantly affected by gender and cultivar. Female cultivar Galhosa had the highest levels of phenolic compounds, and the highest antioxidant activity. Extracts from the hermaphrodite trees and from the female cultivars Galhosa and Costela/Canela exhibited the highest cytotoxic activity. The most abundant compound was theophylline. The phenolic content was correlated to both antioxidant and cytotoxic activities. Our findings provide new knowledge about the health implications of consuming food supplemented with carob germ flour.

  18. Profile of non-volatiles in whisky with regard to superoxide dismutase activity.

    Science.gov (United States)

    Koga, Kunimasa; Tachihara, Satoshi; Shirasaka, Norifumi; Yamada, Yuri; Koshimizu, Sei-Ichi

    2011-08-01

    SOD (Superoxide dismutase)-like activities of 23 kinds of single malt whisky (Scotch and Japanese) were evaluated. There was a positive correlation between SOD-like activity and the maturation age of whisky that exceeded the difference resulting from the manufacturing region. The SOD-like activity of Yamazaki 18, a typical single malt whisky in Japan, was approximately 1333 U/ml and that of non-volatile components in the whisky was 388U/mg, indicating that single malt whisky generally has a very strong SOD-like activity. To elucidate their contribution to SOD-like activity, the non-volatile components of whisky (Yamazaki 18) were ultrafiltered and separated with a Diaion HP20/water-EtOH system. Elution of the fraction less than 5000 molecular weight (whisky. As this elution contained a considerable amount of polyphenolics, the content and SOD-like specific activity of ellagic acid, gallic acid, and lyoniresinol--the main whisky polyphenolics--were evaluated. The contribution of these compounds to the SOD-like activity of whisky was approximately 15%. Polyphenolics in whisky were relatively distributed to a higher MW fraction compared to carbohydrates in whisky, and specific activity (SOD-like activity per weight) of the >10,000 MW fraction was greater than that of the whisky together with main whisky polyphenolics.

  19. Effects of Iron Overload on the Activity of Na,K-ATPase and Lipid Profile of the Human Erythrocyte Membrane.

    Directory of Open Access Journals (Sweden)

    Leilismara Sousa

    Full Text Available Iron is an essential chemical element for human life. However, in some pathological conditions, such as hereditary hemochromatosis type 1 (HH1, iron overload induces the production of reactive oxygen species that may lead to lipid peroxidation and a change in the plasma-membrane lipid profile. In this study, we investigated whether iron overload interferes with the Na,K-ATPase activity of the plasma membrane by studying erythrocytes that were obtained from the whole blood of patients suffering from iron overload. Additionally, we treated erythrocytes of normal subjects with 0.8 mM H2O2 and 1 μM FeCl3 for 24 h. We then analyzed the lipid profile, lipid peroxidation and Na,K-ATPase activity of plasma membranes derived from these cells. Iron overload was more frequent in men (87.5% than in women and was associated with an increase (446% in lipid peroxidation, as indicated by the amount of the thiobarbituric acid reactive substances (TBARS and an increase (327% in the Na,K-ATPase activity in the plasma membrane of erythrocytes. Erythrocytes treated with 1 μM FeCl3 for 24 h showed an increase (132% in the Na,K-ATPase activity but no change in the TBARS levels. Iron treatment also decreased the cholesterol and phospholipid content of the erythrocyte membranes and similar decreases were observed in iron overload patients. In contrast, erythrocytes treated with 0.8 mM H2O2 for 24 h showed no change in the measured parameters. These results indicate that erythrocytes from patients with iron overload exhibit higher Na,K-ATPase activity compared with normal subjects and that this effect is specifically associated with altered iron levels.

  20. Effects of Iron Overload on the Activity of Na,K-ATPase and Lipid Profile of the Human Erythrocyte Membrane

    Science.gov (United States)

    Sousa, Leilismara; Garcia, Israel J. P.; Costa, Tamara G. F.; Silva, Lilian N. D.; Renó, Cristiane O.; Oliveira, Eneida S.; Tilelli, Cristiane Q.; Santos, Luciana L.; Cortes, Vanessa F.; Santos, Herica L.; Barbosa, Leandro A.

    2015-01-01

    Iron is an essential chemical element for human life. However, in some pathological conditions, such as hereditary hemochromatosis type 1 (HH1), iron overload induces the production of reactive oxygen species that may lead to lipid peroxidation and a change in the plasma-membrane lipid profile. In this study, we investigated whether iron overload interferes with the Na,K-ATPase activity of the plasma membrane by studying erythrocytes that were obtained from the whole blood of patients suffering from iron overload. Additionally, we treated erythrocytes of normal subjects with 0.8 mM H2O2 and 1 μM FeCl3 for 24 h. We then analyzed the lipid profile, lipid peroxidation and Na,K-ATPase activity of plasma membranes derived from these cells. Iron overload was more frequent in men (87.5%) than in women and was associated with an increase (446%) in lipid peroxidation, as indicated by the amount of the thiobarbituric acid reactive substances (TBARS) and an increase (327%) in the Na,K-ATPase activity in the plasma membrane of erythrocytes. Erythrocytes treated with 1 μM FeCl3 for 24 h showed an increase (132%) in the Na,K-ATPase activity but no change in the TBARS levels. Iron treatment also decreased the cholesterol and phospholipid content of the erythrocyte membranes and similar decreases were observed in iron overload patients. In contrast, erythrocytes treated with 0.8 mM H2O2 for 24 h showed no change in the measured parameters. These results indicate that erythrocytes from patients with iron overload exhibit higher Na,K-ATPase activity compared with normal subjects and that this effect is specifically associated with altered iron levels. PMID:26197432

  1. Ensuring the principle of visibility when examining graphic disciplines

    Directory of Open Access Journals (Sweden)

    Tel’noy Viktor Ivanovich

    2015-11-01

    Full Text Available The article shows the importance of the use of didactic principle of visualization in the study of graphic disciplines for more effective organization of educational process, improvement of forms, methods and means of education. The authors analyze different approaches to the classification of means of visualization in modern pedagogy. The proposed classification of clarity with regard to graphic disciplines can be used not so much for their classification, as for the full and effective use of their capabilities in the learning process. The article demonstrates structural links between the stages of clarity, use of funds, ways and rules of their use, leading to successful achievement of the goals for the revitalization of the educational process and enhancing cognitive interest of students. Practical recommendations for the integrated use of means of presentation in the classes on descriptive geometry, engineering graphics and computer graphics are given. Special attention in the learning process is paid to the role of the teacher. In addition to his or her professional knowledge, a teacher should possess oratory skills, to competently combine the rhetoric and psychological techniques to use interactive and effective active forms of training, including workshops, to engage students in the learning process, to monitor feedback from the students’ audience. When conducting different kinds of practice, teachers should know the advantages and disadvantages, strengths and weaknesses, timely application of every means of presentation for greater impact and effect in the educational process. The effectiveness of using the selected visualization tools is largely determined by the methods and techniques of their use in the classroom. It is important to consider the following factors: location, convenient for review, and approach; the accessibility; the expert support of a demonstration by the review; the duration of the demonstration; training students

  2. The essential oil profiles and antibacterial activity of six wild Cinnamomum species.

    Science.gov (United States)

    Vairappan, Charles Santhanaraju; Nagappan, Thilahgavani; Kulip, Julius

    2014-09-01

    The essential oil composition of six species of wild Cinnamomum found in Borneo was investigated. The oils were obtained from bark by hydrodistillation and the volatile chemical profile was obtained via Gas Chromatography-Mass Spectrometry (GCMS). A total of 65 volatile constituents were identified, where the essential oils of the studied specimens contained high contents of oxygenated monoterpenes. Eucalyptol (1.2-31.1%), terpinen-4-ol (7.9-22.1%), eugenol (0.4-37.9%) and α-cadinol (0.4-1.8%) were detected consistently in the specimens studied. The oils of C. cuspidatum and C. crassinervium exhibited significant inhibition against Listeria monocytogenes, specifically the latter, which displayed a lower minimum bactericidal concentration (MBC) value against Staphylococcus aereus and L. monocytogenes. This result had highlighted the possible usage of the essential oil derived from wild cinnamom species against food borne pathogens.

  3. Evaluation of Antioxidant, Antidiabetic and Anticholinesterase Activities of Smallanthus sonchifolius Landraces and Correlation with Their Phytochemical Profiles.

    Science.gov (United States)

    Russo, Daniela; Valentão, Patrícia; Andrade, Paula B; Fernandez, Eloy C; Milella, Luigi

    2015-07-31

    The present study aimed to investigate the phytochemical profile of leaf methanol extracts of fourteen Smallanthus sonchifolius (yacon) landraces and their antioxidant, anticholinesterase and antidiabetic activities that could lead to the finding of more effective agents for the treatment and management of Alzheimer's disease and diabetes. For this purpose, antioxidant activity was assessed using different tests: ferric reducing ability power (FRAP), 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide (˙NO) and superoxide (O2˙-) scavenging and lipid peroxidation inhibition assays. Anticholinesterase activity was investigated by quantifying the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities, whereas antidiabetic activity was investigated by α-amylase and α-glucosidase inhibition tests. To understand the contribution of metabolites, phytochemical screening was also performed by high performance liquid chromatography-diode array detector (HPLC-DAD) system. Among all, methanol extract of PER09, PER04 and ECU44 landraces exhibited the highest relative antioxidant capacity index (RACI). ECU44 was found to be rich in 4,5-di-O-caffeoylquinic acid (CQA) and 3,5-di-O-CQA and displayed a good α-amylase and α-glucosidase inhibition, showing the lowest IC50 values. Flavonoids, instead, seem to be involved in the AChE and BChE inhibition. The results of this study revealed that the bioactive compound content differences could be determinant for the medicinal properties of this plant especially for antioxidant and antidiabetic activities.

  4. First Detection of Linear Polarization in the Line Profiles of Active Cool Stars

    NARCIS (Netherlands)

    Kochukhov, O.; Makaganiuk, V.; Piskunov, N.; Snik, F.; Jeffers, S.V.; Johns-Krull, C. M.; Keller, C.U.; Rodenhuis, M.; Valenti, J. A.

    2011-01-01

    The application of high-resolution spectropolarimetry has led to major progress in understanding the magnetism and activity of late-type stars. During the last decade, magnetic fields have been discovered and mapped for many types of active cool stars using spectropolarimetric data. However, these o

  5. Characterization of the Chemical Constitution and Profile of Pharmacological Activity of PGBx.

    Science.gov (United States)

    1980-04-30

    Mitochondrial Oxydative Phosphorylation System Columna Percent Activity- Comments Fractiorr 20 g 40 4g 7 50 100 slightly lower specific activity 8 115 115...with ether, pithed and artificially ventilated. A stimula- ting electrode was introduced irte the vertebral canal via the right or- bit and an

  6. Activity Profiles in International Female Team Handball Using PlayerLoad(TM).

    Science.gov (United States)

    Wik, Eirik H; Luteberget, Live S; Spencer, Matt

    2016-12-14

    Team handball matches place diverse physical demands on players, which may result in fatigue and decreased activity levels. However, previous speed-based methods of quantifying player activity may not be sensitive for capturing short-lasting team handball-specific movements.

  7. The effect of sauna bathing on lipid profile in young, physically active, male subjects

    Directory of Open Access Journals (Sweden)

    Dorota Gryka

    2014-08-01

    Full Text Available Objectives: The aim of the study was to evaluate effects of Finnish sauna bathing on lipid profile in healthy, young men. Material and Methods: Sixteen male subjects (20–23 years were subjected to 10 sauna bathing sessions in a Finnish sauna every 1 or 2 days. The mean sauna temperature was 90±2°C, while humidity was 5–16%. Each session consisted of three 15-minute parts and a 2-minute cool-down between them. The following measurements were taken before and after the sauna sessions: body mass, heart rate, body skinfold thickness. The percentage fat content and then, the lean body mass were calculated. Total cholesterol, triacylglycerols, lipoprotein cholesterol LDL and HDL were measured in blood samples. Results: A statistically significant decrease of total cholesterol and LDL cholesterol was observed during 3 weeks of sauna treatment and in the week afterwards. A significant decline in triacylglycerols was found directly after the 1st and 24 h directly after the 10th sauna session. After the 10th sauna session the level of HDL cholesterol remained slightly increased, but this change was not statistically significant. A decrease in blood plasma volume was found directly after the 1st and the last sauna bathing session due to perspiration. An adaptive increase in blood plasma volume was also found after the series of 10 sauna sessions. Conclusions: Ten complete sauna bathing sessions in a Finnish sauna caused a reduction in total cholesterol and LDL cholesterol fraction levels during the sessions and a gradual return of these levels to the initial level during the 1st and the 2nd week after the experiment. A small, statistically insignificant increase in HDL-C level and a transient decline in triacylglycerols were observed after those sauna sessions. The positive effect of sauna on lipid profile is similar to the effect that can be obtained through a moderate-intensity physical exercise.

  8. Graphical user interface prototyping for distributed requirements engineering

    CERN Document Server

    Scheibmayr, Sven

    2014-01-01

    Finding and understanding the right requirements is essential for every software project. This book deals with the challenge to improve requirements engineering in distributed software projects. The use of graphical user interface (GUI) prototypes can help stakeholders in such projects to elicit and specify high quality requirements. The research objective of this study is to develop a method and a software artifact to support the activities in the early requirements engineering phase in order to overcome some of the difficulties and improve the quality of the requirements, which should eventu

  9. First detection of linear polarization in the line profiles of active cool stars

    CERN Document Server

    Kochukhov, O; Piskunov, N; Snik, F; Jeffers, S V; Johns-Krull, C M; Keller, C U; Rodenhuis, M; Valenti, J A

    2011-01-01

    The application of high-resolution spectropolarimetry has led to major progress in understanding the magnetism and activity of late-type stars. During the last decade, magnetic fields have been discovered and mapped for many types of active cool stars using spectropolarimetric data. However, these observations and modeling attempts are fundamentally incomplete since they are based on the interpretation of the circular polarization alone. Taking advantage of the newly built HARPS polarimeter, we have obtained the first systematic observations of several cool active stars in all four Stokes parameters. Here we report the detection of magnetically-induced linear polarization for the primary component of the very active RS CVn binary HR 1099 and for the moderately active K dwarf epsilon Eri. For both stars the amplitude of linear polarization signatures is measured to be ~10^{-4} of the unpolarized continuum, which is approximately a factor of ten lower than for circular polarization. This is the first detection ...

  10. Site-specific profiles of estrogenic activity in agricultural areas of California's inland waters.

    Science.gov (United States)

    Lavado, Ramon; Loyo-Rosales, Jorge E; Floyd, Emily; Kolodziej, Edward P; Snyder, Shane A; Sedlak, David L; Schlenk, Daniel

    2009-12-15

    To evaluate the occurrence and sources of compounds capable of feminizing fish in agriculturally impacted waterways of the Central Valley of California, water samples were extracted and subjected to chemical analyses as well as in vitro and in vivo measurements of vitellogenin in juvenile rainbow trout (Oncorhynchus mykiss). Among the 16 sites sampled, 6 locations frequently exhibited elevated concentrations of estrogenic substances with 17beta-estradiol equivalents up to 242 ng/L in vitro and 12 microg/kg in vivo. The patterns of activity varied among sites, with two sites showing elevated activity only in vitro, two showing elevated activity only in vivo, and two showing elevated activity in both assays. Sequential elution of solid-phase extraction (SPE) disks followed by bioassay-guided fractionation was used to characterize water samples from the two locations where activity was observed in both bioassays. The highest estrogenic activity was observed in the most nonpolar fractions (80-100% methanol eluent) from the Napa River, while most of the activity in the Sacramento River Delta eluted in the 60% methanol eluent. Quantitative analyses of SPE extracts and additional HPLC fractionation of the SPE extracts by GC-MS/MS and LC-MS/MS indicated concentrations of steroid hormones, alkylphenol polyethoxylates, and herbicides that were at least 1-3 orders of magnitude below bioassay 17beta-estradiol equivalent calculations. Given the different patterns of activity and chemical properties of the estrogenic compounds, it appears that estrogenic activity in these agriculturally impacted surface waters is attributable to multiple compounds. Further investigation is needed to identify the compounds causing the estrogenic activity and to determine the potential impacts of these compounds on feral fish.

  11. Graphic Notation in Music Therapy: A Discussion of What to Notate in Graphic Notation and How

    Directory of Open Access Journals (Sweden)

    Carl Bergstrøm-Nielsen

    2009-01-01

    Full Text Available This article presents graphic notations of music and related forms of communication in music therapy contexts, created by different authors and practitioners. Their purposes, objects of description, and the elements of graphic language are reflected upon in a comparative discussion. From that it becomes clear that the aspect of overview is a fundamental one, facilitating perception of complex data. This also makes possible to memorise complex data, extending the natural limits of human memory. Discovering hidden aspects in the clinical data, as well as sharing and communicating these aspects are also important concerns. Among the authors discussed, there is a large variety both in goals and methods. Keywords are proposed to circumscribe moments of possible interest connected to graphic notations. I suggest that the discipline of graphic notation can be useful for the grounding of music therapy presentation and research in empirical, clinical-musical reality, and welcome further discussion and explorative work.

  12. Effects of different methods of DNA extraction for activated sludge on the subsequent analysis of bacterial community profiles.

    Science.gov (United States)

    Sun, Lianpeng; Ouyang, Xiong; Tang, Yueheng; Yang, Ying; Luo, Ying

    2012-02-01

    The effect of different DNA extraction protocols on activated sludge DNA yield and bacterial community composition was evaluated by temperature gradient gel electrophoresis (TGGE). Nine different procedures to extract DNA were compared-sonication (30s), sonication (40s), sonication (50s), freezing-thawing, bead milling, sodium dodecyl sulfate (SDS)-lysozyme, SDS-proteinase K, SDS-lysozyme-proteinase, and a commercial extraction kit. It was found that the TGGE profiles and the DNA band numbers made significant differences via various extraction methods. The yield and purity of DNA extracted by sonication and other physical methods were not satisfactory, while the DNA purity extracted by SDS and other chemical-biological methods were better. Crude DNA extracts isolated by sonication and other physical methods passed the polymerase chain reaction, despite the absence of purification and acquired affluent DNA bands in TGGE. The affluence of bands in TGGE was not consistent with the yield and purification of DNA, but was correlative with extraction protocols. To analyze the activated sludge bacterial community by TGGE fingerprint, it is necessary to make a synthesis of the TGGE fingerprint profiles of chemical and physical DNA extraction methods to overcome the representative bias.

  13. Phosphoproteomics Profiling of Tobacco Mature Pollen and Pollen Activated in vitro.

    Science.gov (United States)

    Fíla, Jan; Radau, Sonja; Matros, Andrea; Hartmann, Anja; Scholz, Uwe; Feciková, Jana; Mock, Hans-Peter; Čapková, Věra; Zahedi, René Peiman; Honys, David

    2016-04-01

    Tobacco mature pollen has extremely desiccated cytoplasm, and is metabolically quiescent. Upon re-hydration it becomes metabolically active and that results in later emergence of rapidly growing pollen tube. These changes in cytoplasm hydration and metabolic activity are accompanied by protein phosphorylation. In this study, we subjected mature pollen, 5-min-activated pollen, and 30-min-activated pollen to TCA/acetone protein extraction, trypsin digestion and phosphopeptide enrichment by titanium dioxide. The enriched fraction was subjected to nLC-MS/MS. We identified 471 phosphopeptides that carried 432 phosphorylation sites, position of which was exactly matched by mass spectrometry. These 471 phosphopeptides were assigned to 301 phosphoproteins, because some proteins carried more phosphorylation sites. Of the 13 functional groups, the majority of proteins were put into these categories: transcription, protein synthesis, protein destination and storage, and signal transduction. Many proteins were of unknown function, reflecting the fact that male gametophyte contains many specific proteins that have not been fully functionally annotated. The quantitative data highlighted the dynamics of protein phosphorylation during pollen activation; the identified phosphopeptides were divided into seven groups based on the regulatory trends. The major group comprised mature pollen-specific phosphopeptides that were dephosphorylated during pollen activation. Several phosphopeptides representing the same phosphoprotein had different regulation, which pinpointed the complexity of protein phosphorylation and its clear functional context. Collectively, we showed the first phosphoproteomics data on activated pollen where the position of phosphorylation sites was clearly demonstrated and regulatory kinetics was resolved.

  14. Extracellular enzymatic activities and physiological profiles of yeasts colonizing fruit trees.

    Science.gov (United States)

    Molnárová, Jana; Vadkertiová, Renáta; Stratilová, Eva

    2014-07-01

    Yeasts form a significant and diverse part of the phyllosphere microbiota. Some yeasts that inhabit plants have been found to exhibit extracellular enzymatic activities. The aim of the present study was to investigate the ability of yeasts isolated from leaves, fruits, and blossoms of fruit trees cultivated in Southwest Slovakia to produce extracellular enzymes, and to discover whether the yeasts originating from these plant organs differ from each other in their physiological properties. In total, 92 strains belonging to 29 different species were tested for: extracellular protease, β-glucosidase, lipase, and polygalacturonase activities; fermentation abilities; the assimilation of xylose, saccharose and alcohols (methanol, ethanol, glycerol); and for growth in a medium with 33% glucose. The black yeast Aureobasidium pullulans showed the largest spectrum of activities of all the species tested. Almost 70% of the strains tested demonstrated some enzymatic activity, and more than 90% utilized one of the carbon compounds tested. Intraspecies variations were found for the species of the genera Cryptococcus and Pseudozyma. Interspecies differences of strains exhibiting some enzymatic activities and utilizing alcohols were also noted. The largest proportion of the yeasts exhibited β-glucosidase activity and assimilated alcohols independently of their origin. The highest number of strains positive for all activities tested was found among the yeasts associated with leaves. Yeasts isolated from blossoms assimilated saccharose and D-xylose the most frequently of all the yeasts tested. The majority of the fruit-inhabiting yeasts grew in the medium with higher osmotic pressure.

  15. Bioactive metabolite profiles and antimicrobial activity of ethanolic extracts from Muntingia calabura L. leaves and stems

    Institute of Scientific and Technical Information of China (English)

    William Patrick Cruiz Buhian; Raquel Orejudos Rubio; Demetrio Lim Valle Jr.; Juliana Janet Martin-Puzon

    2016-01-01

    Objective: To determine the bioactive phytochemicals and antimicrobial activity of leaf and stem ethanolic extracts from Muntingia calabura L.(M. calabura).Methods: Dried leaves and stems of M. calabura were extracted with 95% ethanol. The antibacterial and antifungal activities of the extracts were examined using the disc diffusion assay. The minimum inhibitory concentration(MIC) of each extract showing antimicrobial activity was determined. The dried extracts were subjected to phytochemical screening to determine the presence of bioactive components. Total phenolic and flavonoid contents were also determined by the Folin–Ciocalteu method and the aluminum chloride method, respectively.Results: Varying degrees of antimicrobial activity were exhibited by the leaf and stem extracts against Pseudomonas aeruginosa(P. aeruginosa), Salmonella typhimurium,Staphylococcus aureus(S. aureus), Bacillus subtilis, and Candida albicans(C. albicans),with minimal activity against Escherichia coli. Based on the MIC, the extracts showed the highest activity against C. albicans, S. aureus and P. aeruginosa. Phytochemical screening revealed the presence of sterols, flavonoids, alkaloids, saponins, glycosides and tannins in the leaf extract; however, no triterpenes were detected. In the stem extract,triterpenes were detected along with relative amounts of flavonoids, saponins, glycosides and tannins. Alkaloids and sterols were absent in the stem extract.Conclusions: M. calabura leaf and stem ethanol extracts are potential sources of antibacterial agents against P. aeruginosa and S. aureus. This study reports for the first time the high degree of antifungal activity of M. calabura ethanolic extract, especially against C. albicans.

  16. Transcriptional Profiling of Vibrio parahaemolyticus exsA reveals a complex activation network for type III secretion

    Directory of Open Access Journals (Sweden)

    Aaron C. Liu

    2015-10-01

    Full Text Available Vibrio parahaemolyticus (Vp is a marine halophilic bacterium that is commonly associated with oysters and shrimp. Human consumption of contaminated shellfish can result in Vp mediated gastroenteritis and severe diarrheal disease. Vp encodes two type 3 secretion systems (T3SS-I and T3SS-II that have been functionally implicated in cytotoxicity and enterotoxicity respectively. In this study, we profiled protein secretion and temporal promoter activities associated with exsA and exsB gene expression. exsA is an AraC-like transcriptional activator that is critical for activating multiple operons that encode T3SS-1 genes, whereas exsB is thought to encode an outer membrane pilotin component for T3SS-1. The exsBA genetic locus has two predicted promoter elements. The predicted exsB and exsA promoters were individually cloned upstream of luxCDABE genes in reporter plasmid constructs allowing for in situ, real-time quantitative light emission measurements under many growth conditions. Low calcium growth conditions supported maximal exsB and exsA promoter activation. exsB promoter activity exhibited high basal activity and resulted in an exsBA co-transcript. Furthermore, a separate proximal exsA promoter showed initial low basal activity yet eventually exceeded that of exsB and reached maximal levels after 2.5 hours corresponding to an entry into early log phase. exsA promoter activity was significantly higher at 30oC than 37oC, which also coincided with increased secretion levels of specific T3SS-1 effector proteins. Lastly, bioinformatic analyses identified a putative expanded ExsA binding motif for multiple transcriptional operons. These findings suggest a two wave model of Vp T3SS-I induction that integrates two distinct promoter elements and environmental signals into a complex ExsA activation framework.

  17. Variations in Tunisian borage essential oil profiles and their antioxidant activities during flowering.

    Science.gov (United States)

    Salem, Nidhal; Msaada, Kamel; Hammami, Majdi; Jday, Ahmad; Salem, Sahar; Limam, Ferid; Marzouk, Brahim

    2014-01-01

    This study was conducted to examine the chemical composition and antioxidant activity of the essential oils (EOs) of Borago officinalis stem during its flowering stage. The EO composition was characterised by high proportions of E,E-decadienal, the main compound of monoterpene hydrocarbon class, with values varying from 47.08% to 55.28% in two Tunisian regions. The region of Korba exhibited the highest scavenging activity (2.05 mg/mL) by comparison with Beja region. In all tests, the fructification stage showed the best antioxidant activity of all flowering stages.

  18. Organelle-Specific Activity-Based Protein Profiling in Living Cells

    Energy Technology Data Exchange (ETDEWEB)

    Wiedner, Susan D.; Anderson, Lindsey N.; Sadler, Natalie C.; Chrisler, William B.; Kodali, Vamsi K.; Smith, Richard D.; Wright, Aaron T.

    2014-02-06

    A multimodal acidic organelle targeting activity-based probe was developed for analysis of subcellular native enzymatic activity of cells by fluorescent microscopy and mass spectrometry. A cathepsin reactive warhead was conjugated to an acidotropic amine, and a clickable alkyne for appendage of AlexaFluor 488 or biotin reporter tags. This probe accumulated in punctate vesicles surrounded by LAMP1, a lysosome marker, as observed by Structured Illumination Microscopy (SIM) in J774 mouse macrophage cells. Biotin conjugation, affinity purification, and analysis of in vivo labeled J774 by mass spectrometry showed that the probe was very selective for Cathepsins B and Z, two lysosomal cysteine proteases. Analysis of starvation induced autophagy, which is an increase in cell component catabolism involving lysosomes, showed a large increase in tagged protein number and an increase in cathepsin activity. Organelle targeting activity-based probes and subsequent analysis of resident proteins by mass spectrometry is enabled by tuning the physicochemical properties of the probe.

  19. Health profile for Danish adults with activity limitation and/or physical disability

    DEFF Research Database (Denmark)

    Johnsen, Nina Føns; Davidsen, Michael; Juel, Knud

    proportion of Danish adults with activity limitation and/or physical disabilities experiences a good health and well-being, a larger proportion has an unhealthy lifestyle, poor social relations and uses the health care system frequently, as compared to adults without activity limitation and/or physical...... limitation and/or disabilities have a poor health and social contact. Availability and flexibility of health care services and prevention programs should be prioritized....

  20. Comparison of survey methods to profile participants in emerging adventure recreation activities undertaken in wilderness

    OpenAIRE

    Burgin, Shelley; Hardiman, Nigel

    2014-01-01

    Growth in ‘adventure recreation’, typically practised in protected areas, is occurring. Canyoning (cf. canyoneering), is one such activity. In the Greater Blue Mountain World Heritage Area (GBMWHA), Australia, management was concerned that canyoning was causing environmental damage. However, there is a dearth of data, even on participation, because of the ‘composite’ nature of the activity, its recent emergence, and because adventure recreation is typically restricted to wilderness areas whic...

  1. Development and Psychometric Evaluation of the Treatment-Emergent Activation and Suicidality Assessment Profile

    OpenAIRE

    Reid, Jeannette M.; Storch, Eric A.; Murphy, Tanya K.; Bodzin, Danielle; Mutch, P. Jane; Lehmkuhl, Heather; Aman, Michael; Goodman, Wayne K.

    2010-01-01

    Although effective in treating a range of childhood psychiatric conditions, selective serotonin reuptake inhibitors (SSRI) have been implicated in the induction of an “activation syndrome” (characterized by symptoms of irritability, restlessness, emotional labiality, etc.) that may represent an intermediary state change that fosters suicidality. SSRI-induced activation syndrome is well-accepted by many clinicians and thought to be relatively common, particularly in children and teens. However...

  2. Fibromyalgia in patients with axial spondyloarthritis: epidemiological profile and effect on measures of disease activity.

    Science.gov (United States)

    Salaffi, Fausto; De Angelis, Rossella; Carotti, Marina; Gutierrez, Marwin; Sarzi-Puttini, Piercarlo; Atzeni, Fabiola

    2014-08-01

    To determine the prevalence of fibromyalgia (FM) in patients with ankylosing spondylitis (AS) or psoriatic arthritis (PsA) characterized by axial involvement (axial-PsA), and to assess the discriminative ability of different versions of the Ankylosing Spondylitis Disease Activity Score (ASDAS) and the Bath Ankylosing Spondylitis Activity Disease Activity Index (BASDAI) in measuring disease activity in three different cohorts of patients with axial spondyloarthritis (axial-SpA), FM, or both (axial-SpA + FM), this study was divided into two phases: (1) 402 patients with definite AS or axial-PsA were examined to diagnose FM and estimate its prevalence; and (2) 419 patients (111 with axial-SpA, 248 with FM, and 60 with aSpA + FM) were evaluated using the different versions of the ASDAS and BASDAI to assess the effect on disease activity. The overall prevalence of FM in the axial-SpA population was 14.9 %, significantly higher among women (p axial-PsA was 17.2 %. Although the BASDAI scores correlated with those of ASDAS-C-reactive protein (CRP) and ASDAS-erythrocyte sedimentation rate (ESR) (p axial-SpA and more prevalent in female patients. Our findings suggest that ASDAS is better than BASDAI in distinguishing patients with disease activity from those with functional impairment. The use of ASDAS may be very useful in clinical practice as it allows treating patients with the most appropriate therapy.

  3. Metabolic Profiling of Total Physical Activity and Sedentary Behavior in Community-Dwelling Men

    Science.gov (United States)

    Harada, Sei; Iida, Miho; Kurihara, Ayako; Takeuchi, Ayano; Kuwabara, Kazuyo; Sugiyama, Daisuke; Okamura, Tomonori; Akiyama, Miki; Nishiwaki, Yuji; Oguma, Yuko; Suzuki, Asako; Suzuki, Chizuru; Hirayama, Akiyoshi; Sugimoto, Masahiro; Soga, Tomoyoshi; Tomita, Masaru; Takebayashi, Toru

    2016-01-01

    Objective Physical activity is known to be preventive against various non-communicable diseases. We investigated the relationship between daily physical activity level and plasma metabolites using a targeted metabolomics approach in a population-based study. Methods A total of 1,193 participants (male, aged 35 to 74 years) with fasting blood samples were selected from the baseline survey of a cohort study. Information on daily total physical activity, classified into four levels by quartile of metabolic equivalent scores, and sedentary behavior, defined as hours of sitting per day, was collected through a self-administered questionnaire. Plasma metabolite concentrations were quantified by capillary electrophoresis mass spectrometry method. We performed linear regression analysis models with multivariable adjustment and corrected p-values for multiple testing in the original population (n = 808). The robustness of the results was confirmed by replication analysis in a separate population (n = 385) created by random allocation. Results Higher levels of total physical activity were associated with various metabolite concentrations, including lower concentrations of amino acids and their derivatives, and higher concentrations of pipecolate (FDR p valine, 4-methyl-2-oxoisopentanoate, 2-oxoisopentanoate, alanine, and proline concentrations were lower with a shorter sitting time. Conclusions Physical activity is related to various plasma metabolites, including known biomarkers for future insulin resistance or type 2 diabetes. These metabolites might potentially play a key role in the protective effects of higher physical activity and/or less sedentary behavior on non-communicable diseases. PMID:27741291

  4. Engineering Graphics Education: Webcomics as a Tool to Improve Weaker Students’ Motivation

    Directory of Open Access Journals (Sweden)

    Riccardo Metraglia

    2014-05-01

    Full Text Available In engineering colleges, first-year students come from different kinds of high school and have different technical backgrounds. In engineering graphics courses, the weaker students are the ones entering with a lower technical background. Such students are less motivated and have generally difficulties in keeping high their attention level during the lessons. In this study, the use of a webcomics structured in graphic novels was experimented as a motivational support in an engineering graphics course. Sixty nine students of a class taught by using webcomics as support and 47 students of a class taught traditionally were classified according to the kind of their high school of provenience: technical; scientific; non-technical and non-scientific. The findings showed that in the class where webcomics were used, students from non-technical and non-scientific high school scored a higher level of attention compared to others. The teacher who used the webcomics commented it an effective tool to encourage and stimulate weaker learners to actively participate to the lessons and the majority of students agreed such tool was stimulating. At the same time, some students considered the webcomics representation of engineering graphics topics as too far from the reality. It is concluded that the use of webcomics structured in graphic novels is a proficient way to better motivate weaker students to arouse and keep their attention at a high level during engineering graphics lessons.

  5. Patterns of longitudinal neural activity linked to different cognitive profiles in Parkinson’s disease

    Directory of Open Access Journals (Sweden)

    Atsuko Nagano-Saito

    2016-11-01

    Full Text Available Mild cognitive impairment in Parkinson’s disease (PD has been linked with functional brain changes. Previously, using functional magnetic resonance imaging (fMRI, we reported reduced cortico-striatal activity in patients with PD who also had mild cognitive impairment (MCI versus those who did not (non-MCI. We followed up these patients to investigate the longitudinal effect on the neural activity. Twenty-four non-demented patients with Parkinson’s disease (non-MCI: 12, MCI; 12 were included in the study. Each participant underwent two fMRIs while performing the Wisconsin Card Sorting Task 20 months apart. The non-MCI patients recruited the usual cognitive corticostriatal loop at the first and second sessions (Time 1 and Time 2, respectively. However, decreased activity was observed in the cerebellum and occipital area and increased activity was observed in the medial prefrontal cortex and parietal lobe during planning set-shift at Time 2. Increased activity in the precuneus was also demonstrated while executing set-shifts at Time 2. The MCI patients revealed more activity in the frontal, parietal and occipital lobes during planning set-shifts, and in the parietal and occipital lobes, precuneus, and cerebellum, during executing set-shift at Time 2. Analysis regrouping of both groups of PD patients revealed that hippocampal and thalamic activity at Time 1 was associated with less cognitive decline over time. Our results reveal that functional alteration along the time-points differed between the non-MCI and MCI patients. They also underline the importance of preserving thalamic and hippocampal function with respect to cognitive decline over time.

  6. Gene expression profiling in cells with enhanced gamma-secretase activity.

    Directory of Open Access Journals (Sweden)

    Alexandra I Magold

    Full Text Available BACKGROUND: Processing by gamma-secretase of many type-I membrane protein substrates triggers signaling cascades by releasing intracellular domains (ICDs that, following nuclear translocation, modulate the transcription of different genes regulating a diverse array of cellular and biological processes. Because the list of gamma-secretase substrates is growing quickly and this enzyme is a cancer and Alzheimer's disease therapeutic target, the mapping of gamma-secretase activity susceptible gene transcription is important for sharpening our view of specific affected genes, molecular functions and biological pathways. METHODOLOGY/PRINCIPAL FINDINGS: To identify genes and molecular functions transcriptionally affected by gamma-secretase activity, the cellular transcriptomes of Chinese hamster ovary (CHO cells with enhanced and inhibited gamma-secretase activity were analyzed and compared by cDNA microarray. The functional clustering by FatiGO of the 1,981 identified genes revealed over- and under-represented groups with multiple activities and functions. Single genes with the most pronounced transcriptional susceptibility to gamma-secretase activity were evaluated by real-time PCR. Among the 21 validated genes, the strikingly decreased transcription of PTPRG and AMN1 and increased transcription of UPP1 potentially support data on cell cycle disturbances relevant to cancer, stem cell and neurodegenerative diseases' research. The mapping of interactions of proteins encoded by the validated genes exclusively relied on evidence-based data and revealed broad effects on Wnt pathway members, including WNT3A and DVL3. Intriguingly, the transcription of TERA, a gene of unknown function, is affected by gamma-secretase activity and was significantly altered in the analyzed human Alzheimer's disease brain cortices. CONCLUSIONS/SIGNIFICANCE: Investigating the effects of gamma-secretase activity on gene transcription has revealed several affected clusters of

  7. Neuroprotective profile of pyridothiazepines with blocking activity of the mitochondrial Na(+)/Ca(2+) exchanger.

    Science.gov (United States)

    Martínez-Sanz, Francisco J; Lajarín-Cuesta, Rocío; González-Lafuente, Laura; Moreno-Ortega, Ana J; Punzón, Eva; Cano-Abad, María F; de los Ríos, Cristóbal

    2016-02-15

    The mitochondrial Na(+)/Ca(2+) exchanger plays an important role in the control of cytosolic Ca(2+) cycling in excitable cells, essential for the regulation of a plethora of Ca(2+)-dependent physio-pathological events, such as apoptosis in the presence of a Ca(2+) overload. There are very few pharmacological tools available to study both physiological and pathological implications of the mitochondrial Na(+)/Ca(2+) exchanger, where the benzothiazepine CGP37157 is the best-known ligand, used since the 1980s. However, it is not an efficient blocker and lacks of selectivity, as also blocks several other cellular Ca(2+) transporters. Moreover, CGP37157 is a very lipophilic drug, showing very poor water solubility, what has hindered its therapeutic use. Attempting to improve its pharmacokinetic profile as well as its potency and selectivity, we herein describe the synthesis of new CGP37157 analogs, where the benzene-fused ring has been replaced by a pyridine. On top of a better water solubility and lower log P value, some of these new pyridothiazepine derivatives also presented a higher capacity to regulate the mitochondrial Ca(2+) clearance, while keeping the neuroprotective properties presented in the head compound CGP37157.

  8. Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation

    Directory of Open Access Journals (Sweden)

    Alessandra Micera

    2016-01-01

    Full Text Available Objective. Vernal keratoconjunctivitis (VKC is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC. Methods. This study cohort included 16 consecutive patients with VKC and 10 controls. Participants were subjected to clinical assessment of ocular surface and tear sampling. Total protein quantification, total protein sketch, and protein array (sixty protein candidates were evaluated. Results. An overall increased Fluorescent Intensity expression was observed in VKC arrays. Particularly, IL1β, IL15, IL21, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, ICAM2, βNGF, NT4, BDNF, βFGF, SCF, MMP1, and MMP2 were increased in quiescent VKC. Of those candidates, only IL1β, IL15, IL21, βNGF, SCF, MMP2, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, and ICAM2 were increased in both active and quiescent VKC. Finally, NT4, βFGF, and MMP1 were highly increased in active VKC. Conclusion. A distinct “protein tear-print” characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states. Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and/or exacerbation out-of-season.

  9. Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation

    Science.gov (United States)

    Micera, Alessandra; Di Zazzo, Antonio; Esposito, Graziana; Sgrulletta, Roberto; Calder, Virginia L.; Bonini, Stefano

    2016-01-01

    Objective. Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC. Methods. This study cohort included 16 consecutive patients with VKC and 10 controls. Participants were subjected to clinical assessment of ocular surface and tear sampling. Total protein quantification, total protein sketch, and protein array (sixty protein candidates) were evaluated. Results. An overall increased Fluorescent Intensity expression was observed in VKC arrays. Particularly, IL1β, IL15, IL21, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, ICAM2, βNGF, NT4, BDNF, βFGF, SCF, MMP1, and MMP2 were increased in quiescent VKC. Of those candidates, only IL1β, IL15, IL21, βNGF, SCF, MMP2, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, and ICAM2 were increased in both active and quiescent VKC. Finally, NT4, βFGF, and MMP1 were highly increased in active VKC. Conclusion. A distinct “protein tear-print” characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states. Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and/or exacerbation out-of-season. PMID:26989694

  10. VEGETATIVE SUPPORT OF CARDIAC ACTIVITY IN ATHLETES WITH DIFFERENT ANTHROPOMETRIC PROFILE

    Directory of Open Access Journals (Sweden)

    O. N. Kudrya

    2016-01-01

    Full Text Available The purpose of research – to study the features of the functioning of the cardiovascular system and regulatory mechanisms of the young athletes of different heights.Materials and methods. The study included athletes aged 15-16 (32 girls and 36 boys engaged in competitive sports. To study the autonomic regulation of the cardiovascular system using mathematical methods and spectral analysis of heart rate variability. To characterize the vegetative support the circulatory apparatus, all subjects performed an active orthostatic test.Results. The features of vegetative maintenance of heart activity in tall athletes: stress regulatory mechanisms observed resting in tall men and decrease the functionality of the sympathetic division of the autonomic nervous system during active orthostatic test in athletes of different sex. Athletes tall urgent adaptation of the cardiovascular system to changing external conditions associated with activation of suprasegmental divisions of the autonomic nervous system and the excessive activation of the sympathetic division, which is an inefficient way of adaptation.Conclusion. Thus, high growth is evident not only in the increase of total size of the body of athletes, but also in the peculiarities of morphofunctional state involved, indicating the need of individual rationing of loads for tall players. The revealed morphofunctional characteristics of the organism tall athletes allow us to recommend an increase in the proportion of aerobic exercise to enhance the adaptive capacities of the organism. 

  11. Profile of oxidant and antioxidant activity in prepubertal children related to age, gender, exercise, and fitness.

    Science.gov (United States)

    Llorente-Cantarero, Francisco Jesus; Gil-Campos, Mercedes; Benitez-Sillero, Juan de Dios; Muñoz-Villanueva, Maria Carmen; Tasset, Inmaculada; Pérez-Navero, Juan Luis

    2013-04-01

    Tissue damage resulting from oxidative stress induced by a pathological condition might have more serious consequences in children than in adults. Researchers have not yet identified particular markers - alone or in combination with others - of oxidative stress, or their role in pediatric diseases. The aim of this study was to identify gender-based biomarkers for measuring oxidative stress. Oxidative biomarkers were studied in 138 healthy Spanish children (85 boys, 53 girls) 7 to 12 years of age, at the prepubertal (Tanner I) stage, independent of body mass index (BMI), age, fitness (measured by 20-m shuttle run test), and physical activity (measured by participation in an after-school exercise program). The oxidative biomarkers measured were lipid peroxidation products, total nitrites, protein carbonyls, and oxidized glutathione (GSSG). The antioxidant biomarkers measured were total glutathione (TG), reduced glutathione (GSH), superoxide dismutase activity (SOD), and glutathione peroxidase activity. In the study population, height, weight, waist circumference, and BMI were lower in girls than in boys. For oxidative biomarkers, boys had higher levels of protein carbonyl than girls (p fitness, and physical activity. In conclusion, prepubertal girls had higher oxidative stress than boys, in addition to higher levels of SOD, independent of age, BMI, fitness, and physical activity.

  12. Effects of germination on the nutritional properties, phenolic profiles, and antioxidant activities of buckwheat.

    Science.gov (United States)

    Zhang, Ge; Xu, Zhicun; Gao, Yuanyuan; Huang, Xianxiao; Zou, Yanping; Yang, Tiankui

    2015-05-01

    Germination is considered to be an effective process for improving the nutritional quality and functionality of cereals. In this study, changes of nutritional ingredients, antinutritional components, chemical composition, and antioxidant activities of buckwheat seeds over 72 h of germination were investigated, and the reasons for these changes are discussed. With the prolonged germination time, the contents of crude protein, reducing sugar, total phenolics, total flavonoids, and condensed tannins increased significantly, while the levels of crude fat, phytic acid, and the activity of trypsin inhibitor decreased. Phenolic compounds, such as rutin, vitexin, isovitexin, orientin, isoorientin, chlorogenic acid, trans-3-hydroxycinnamic acid, and p-hydroxybenzoic acid increased significantly during the germination process, which may be due to the activation of phenylalanine ammonialyase. The improvement of flavonoids led to significant enhancement of the antioxidant activities of germinated buckwheat. Germinated buckwheat had better nutritional value and antioxidant activities than ungerminated buckwheat, and it represented an excellent natural source of flavonoids and phenolic compounds, especially rutin and C-glycosylflavones. Therefore, germinated buckwheat could be used as a promising functional food for health promotion.

  13. Factorial graphical lasso for dynamic networks

    CERN Document Server

    Wit, E C

    2012-01-01

    Dynamic networks models describe a growing number of important scientific processes, from cell biology and epidemiology to sociology and finance. There are many aspects of dynamical networks that require statistical considerations. In this paper we focus on determining network structure. Estimating dynamic networks is a difficult task since the number of components involved in the system is very large. As a result, the number of parameters to be estimated is bigger than the number of observations. However, a characteristic of many networks is that they are sparse. For example, the molecular structure of genes make interactions with other components a highly-structured and therefore sparse process. Penalized Gaussian graphical models have been used to estimate sparse networks. However, the literature has focussed on static networks, which lack specific temporal constraints. We propose a structured Gaussian dynamical graphical model, where structures can consist of specific time dynamics, known presence or abse...

  14. Word-Sized Graphics for Scientific Texts.

    Science.gov (United States)

    Beck, Fabian; Weiskopf, Daniel

    2017-02-24

    Generating visualizations at the size of a word creates dense information representations often called sparklines. The integration of word-sized graphics into text could avoid additional cognitive load caused by splitting the readers' attention between figures and text. In scientific publications, these graphics make statements easier to understand and verify because additional quantitative information is available where needed. In this work, we perform a literature review to find out how researchers have already applied such word-sized representations. Illustrating the versatility of the approach, we leverage these representations for reporting empirical and bibliographic data in three application examples. For interactive Web-based publications, we explore levels of interactivity and discuss interaction patterns to link visualization and text. We finally call the visualization community to be a pioneer in exploring new visualization-enriched and interactive publication formats.

  15. Graphical Model Debugger Framework for Embedded Systems

    DEFF Research Database (Denmark)

    Zeng, Kebin; Guo, Yu; Angelov, Christo K.

    2010-01-01

    Model Driven Software Development has offered a faster way to design and implement embedded real-time software by moving the design to a model level, and by transforming models to code. However, the testing of embedded systems has remained at the code level. This paper presents a Graphical Model...... Debugger Framework, providing an auxiliary avenue of analysis of system models at runtime by executing generated code and updating models synchronously, which allows embedded developers to focus on the model level. With the model debugger, embedded developers can graphically test their design model...... and check the running status of the system, which offers a debugging capability on a higher level of abstraction. The framework intends to contribute a tool to the Eclipse society, especially suitable for model-driven development of embedded systems....

  16. Graphical Models for Optimal Power Flow

    CERN Document Server

    Dvijotham, Krishnamurthy; Chertkov, Michael; Misra, Sidhant; Vuffray, Marc

    2016-01-01

    Optimal power flow (OPF) is the central optimization problem in electric power grids. Although solved routinely in the course of power grid operations, it is known to be strongly NP-hard in general, and weakly NP-hard over tree networks. In this paper, we formulate the optimal power flow problem over tree networks as an inference problem over a tree-structured graphical model where the nodal variables are low-dimensional vectors. We adapt the standard dynamic programming algorithm for inference over a tree-structured graphical model to the OPF problem. Combining this with an interval discretization of the nodal variables, we develop an approximation algorithm for the OPF problem. Further, we use techniques from constraint programming (CP) to perform interval computations and adaptive bound propagation to obtain practically efficient algorithms. Compared to previous algorithms that solve OPF with optimality guarantees using convex relaxations, our approach is able to work for arbitrary distribution networks an...

  17. Graphical Model Debugger Framework for Embedded Systems

    DEFF Research Database (Denmark)

    Zeng, Kebin

    2010-01-01

    Debugger Framework, providing an auxiliary avenue of analysis of system models at runtime by executing generated code and updating models synchronously, which allows embedded developers to focus on the model level. With the model debugger, embedded developers can graphically test their design model......Model Driven Software Development has offered a faster way to design and implement embedded real-time software by moving the design to a model level, and by transforming models to code. However, the testing of embedded systems has remained at the code level. This paper presents a Graphical Model...... and check the running status of the system, which offers a debugging capability on a higher level of abstraction. The framework intends to contribute a tool to the Eclipse society, especially suitable for model-driven development of embedded systems....

  18. HMI conventions for process control graphics.

    Science.gov (United States)

    Pikaar, Ruud N

    2012-01-01

    Process operators supervise and control complex processes. To enable the operator to do an adequate job, instrumentation and process control engineers need to address several related topics, such as console design, information design, navigation, and alarm management. In process control upgrade projects, usually a 1:1 conversion of existing graphics is proposed. This paper suggests another approach, efficiently leading to a reduced number of new powerful process graphics, supported by a permanent process overview displays. In addition a road map for structuring content (process information) and conventions for the presentation of objects, symbols, and so on, has been developed. The impact of the human factors engineering approach on process control upgrade projects is illustrated by several cases.

  19. Graphics performance in rich Internet applications.

    Science.gov (United States)

    Hoetzlein, Rama C

    2012-01-01

    Rendering performance for rich Internet applications (RIAs) has recently focused on the debate between using Flash and HTML5 for streaming video and gaming on mobile devices. A key area not widely explored, however, is the scalability of raw bitmap graphics performance for RIAs. Does Flash render animated sprites faster than HTML5? How much faster is WebGL than Flash? Answers to these questions are essential for developing large-scale data visualizations, online games, and truly dynamic websites. A new test methodology analyzes graphics performance across RIA frameworks and browsers, revealing specific performance outliers in existing frameworks. The results point toward a future in which all online experiences might be GPU accelerated.

  20. Graphical model construction based on evolutionary algorithms

    Institute of Scientific and Technical Information of China (English)

    Youlong YANG; Yan WU; Sanyang LIU

    2006-01-01

    Using Bayesian networks to model promising solutions from the current population of the evolutionary algorithms can ensure efficiency and intelligence search for the optimum. However, to construct a Bayesian network that fits a given dataset is a NP-hard problem, and it also needs consuming mass computational resources. This paper develops a methodology for constructing a graphical model based on Bayesian Dirichlet metric. Our approach is derived from a set of propositions and theorems by researching the local metric relationship of networks matching dataset. This paper presents the algorithm to construct a tree model from a set of potential solutions using above approach. This method is important not only for evolutionary algorithms based on graphical models, but also for machine learning and data mining.The experimental results show that the exact theoretical results and the approximations match very well.

  1. Automatic Palette Identification of Colored Graphics

    Science.gov (United States)

    Lacroix, Vinciane

    The median-shift, a new clustering algorithm, is proposed to automatically identify the palette of colored graphics, a pre-requisite for graphics vectorization. The median-shift is an iterative process which shifts each data point to the "median" point of its neighborhood defined thanks to a distance measure and a maximum radius, the only parameter of the method. The process is viewed as a graph transformation which converges to a set of clusters made of one or several connected vertices. As the palette identification depends on color perception, the clustering is performed in the L*a*b* feature space. As pixels located on edges are made of mixed colors not expected to be part of the palette, they are removed from the initial data set by an automatic pre-processing. Results are shown on scanned maps and on the Macbeth color chart and compared to well established methods.

  2. Rendering Falling Leaves on Graphics Hardware

    Directory of Open Access Journals (Sweden)

    Marcos Balsa

    2008-04-01

    Full Text Available There is a growing interest in simulating natural phenomena in computer graphics applications. Animating natural scenes in real time is one of the most challenging problems due to the inherent complexity of their structure, formed by millions of geometric entities, and the interactions that happen within. An example of natural scenario that is needed for games or simulation programs are forests. Forests are difficult to render because the huge amount of geometric entities and the large amount of detail to be represented. Moreover, the interactions between the objects (grass, leaves and external forces such as wind are complex to model. In this paper we concentrate in the rendering of falling leaves at low cost. We present a technique that exploits graphics hardware in order to render thousands of leaves with different falling paths in real time and low memory requirements.

  3. Publishing on the WWW. Part 6 - Simple graphic manipulation

    OpenAIRE

    Grech, V

    2001-01-01

    Modern graphic manipulation software is quick and simple to use, and allows medical quality graphics to be produced for online publication. This article demonstrates, step by step, how submitted images are processed by the journal in preparation for publication.

  4. Markov chain Monte Carlo methods in directed graphical models

    DEFF Research Database (Denmark)

    Højbjerre, Malene

    Directed graphical models present data possessing a complex dependence structure, and MCMC methods are computer-intensive simulation techniques to approximate high-dimensional intractable integrals, which emerge in such models with incomplete data. MCMC computations in directed graphical models...

  5. VFR Class B Graphics - Aeronautical Information Services Digital Products

    Data.gov (United States)

    Department of Transportation — These graphics are designed to increase safety and aid pilots in gaining situational awareness within existing and redesigned Class B areas. The graphic provides an...

  6. Polyphenolic Profile and Biological Activity of Chinese Hawthorn (Crataegus pinnatifida BUNGE Fruits

    Directory of Open Access Journals (Sweden)

    Tunde Jurikova

    2012-12-01

    Full Text Available Chinese hawthorn (Crataegus pinnatifida Bge. fruits are rich in polyphenols (e.g., epicatechin, procyanidin B2, procyanidin B5, procyanidin C1, hyperoside, isoquercitrin and chlorogenic acid—active compounds that exert beneficial effects. This review summarizes all information available on polyphenolic content and methods for their quantification in Chinese hawthorn berries and the relationships between individual polyphenolic compounds as well. The influence of species or cultivars, the locality of cultivation, the stage of maturity, and extract preparation conditions on the polyphenolic content were discussed as well. Currently, only fruits of C. pinnatifida and C. pinnatifida var. major are included in the Chinese Pharmacopoeia. Recent trials have demonstrated the efficacy of Chinese hawthorn fruit in lowering blood cholesterol and the risk of cardiovascular diseases. The fruit has also demonstrated anti-inflammatory and anti-tumour activities. This review deals mainly with the biological activity of the fruit related to its antioxidant properties.

  7. Antioxidant activities and phenolics profiling of different parts of Carica papaya by LCMS-MS.

    Science.gov (United States)

    Zunjar, V; Mammen, D; Trivedi, B M

    2015-01-01

    This article deals with the comparison of the antioxidant activity of aqueous extracts of various parts of Carica papaya L. The evaluation of total phenolic content and total flavonoid content revealed high antioxidant potential of the seeds and fruits. The free radical-scavenging potential of the aqueous extracts indicated the seeds to have better DPPH-scavenging activity than fruits. The results were augmented by the FRAP activity as well. The phenolics present in the extracts were separated and identified as 5-hydroxy feruloyl quinic acid, acetyl p-coumaryl quinic acid, quercetin-3-O-rhamnoside, syringic acid hexoside, 5-hydroxy caffeic quinic acid, peonidin-3-O-glucoside, sinapic acid-O-hexoside, cyaniding-3-O-glucose and methyl feruloyl glycoside by LCMS-MS technique.

  8. Polyphenolic profile and biological activity of Chinese hawthorn (Crataegus pinnatifida BUNGE) fruits.

    Science.gov (United States)

    Jurikova, Tunde; Sochor, Jiri; Rop, Otakar; Mlcek, Jiri; Balla, Stefan; Szekeres, Ladislav; Adam, Vojtech; Kizek, Rene

    2012-12-06

    Chinese hawthorn (Crataegus pinnatifida Bge.) fruits are rich in polyphenols (e.g., epicatechin, procyanidin B2, procyanidin B5, procyanidin C1, hyperoside, isoquercitrin and chlorogenic acid)--active compounds that exert beneficial effects. This review summarizes all information available on polyphenolic content and methods for their quantification in Chinese hawthorn berries and the relationships between individual polyphenolic compounds as well. The influence of species or cultivars, the locality of cultivation, the stage of maturity, and extract preparation conditions on the polyphenolic content were discussed as well. Currently, only fruits of C. pinnatifida and C. pinnatifida var. major are included in the Chinese Pharmacopoeia. Recent trials have demonstrated the efficacy of Chinese hawthorn fruit in lowering blood cholesterol and the risk of cardiovascular diseases. The fruit has also demonstrated anti-inflammatory and anti-tumour activities. This review deals mainly with the biological activity of the fruit related to its antioxidant properties.

  9. Whole Blood Activation Results in Altered T Cell and Monocyte Cytokine Production Profiles by Flow Cytometry

    Science.gov (United States)

    Crucian, Brian E.; Sams, Clarence F.

    2001-01-01

    An excellent monitor of the immune balance of peripheral circulating cells is to determine their cytokine production patterns in response to stimuli. Using flow cytometry, a positive identification of cytokine producing cells in a mixed culture may be achieved. Recently, the ability to assess cytokine production following a whole-blood activation culture has been described. In this study, whole blood activation was compared to traditional PBMC activation and the individual cytokine secretion patterns for both T cells, T cell subsets and monocytes was determined by flow cytometry. RESULTS: For T cell cytokine assessment (IFNg/IL-10 and IL-21/L-4) following PMA +ionomycin activation: (1) a Significantly greater percentages of T cells producing IFNgamma and IL-2 were observed following whole-blood culture and (2) altered T cell cytokine production kinetics were observed by varying whole blood culture times. Four-color analysiS was used to allow assessment of cytokine production by specific T cell subsets. It was found that IFNgamma production was significantly elevated in the CD3+/CD8+ T cell population as compared to the CD3+/CD8- population following five hours of whole blood activation. Conversely, IL-2 and IL-10 production were Significantly elevated in the CD3+/CD8- T cell population as compared to the CD3+/CD8+ population. Monocyte cytokine production was assessed in both culture systems following LPS activation for 24 hours. A three-color flow cytometric was used to assess two cytokines (IL-1a/IL-12 and TNFa/IL-10) in conjunction with CD14. Nearly all monocytes were stimulated to produce IL-1a, IL-12 and TNFa. equally well in both culture systems, however monocyte production of IL-10 was significantly elevated in whole blood culture as compared to PBMC culture. IL-12 producing monocytes appeared to be a distinct subpopulation of the IL-1a producing set, whereas IL-10 and TNFa producing monocytes were largely mutually exclusive. IL-10 and TNFa producing

  10. Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells.

    Science.gov (United States)

    te Kamp, Verena; Lindner, Ricco; Jahnke, Heinz-Georg; Krinke, Dana; Kostelnik, Katja B; Beck-Sickinger, Annette G; Robitzki, Andrea A

    2015-05-15

    Label-free and non-invasive monitoring of receptor activation and identification of the involved signal pathways in living cells is an ongoing analytic challenge and a great opportunity for biosensoric systems. In this context, we developed an impedance spectroscopy-based system for the activation monitoring of NPY-receptors in living cells. Using an optimized interdigital electrode array for sensitive detection of cellular alterations, we were able for the first time to quantitatively detect the NPY-receptor activation directly without a secondary or enhancer reaction like cAMP-stimulation by forskolin. More strikingly, we could show that the impedimetric based NPY-receptor activation monitoring is not restricted to the Y1-receptor but also possible for the Y2- and Y5-receptor. Furthermore, we could monitor the NPY-receptor activation in different cell lines that natively express NPY-receptors and proof the specificity of the observed impedimetric effect by agonist/antagonist studies in recombinant NPY-receptor expressing cell lines. To clarify the nature of the observed impedimetric effect we performed an equivalent circuit analysis as well as analyzed the role of cell morphology and receptor internalization. Finally, an antagonist based extensive molecular signal pathway analysis revealed small alterations of the actin cytoskeleton as well as the inhibition of at least L-type calcium channels as major reasons for the observed NPY-induced impedance increase. Taken together, our novel impedance spectroscopy based NPY-receptor activation monitoring system offers the opportunity to identify signal pathways as well as for novel versatile agonist/antagonist screening systems for identification of novel therapeutics in the field of obesity and cancer.

  11. Application of ggplot2 to Pharmacometric Graphics.

    Science.gov (United States)

    Ito, K; Murphy, D

    2013-01-01

    Visualization is a powerful mechanism for extracting information from data. ggplot2 is a contributed visualization package in the R programming language, which creates publication-quality statistical graphics in an efficient, elegant, and systematic manner. This article summarizes key features of the package with examples from pharmacometrics and pointers to available resources for learning ggplot2.CPT: Pharmacometrics & Systems Pharmacology (2013) 2, e79; doi:10.1038/psp.2013.56; advance online publication 16 October 2013.

  12. Impressive: printmaking, letterpress and graphic design

    OpenAIRE

    Wood, Philippa

    2010-01-01

    Inspired by the challenge of hands-on tweaks and unpredictable results as well as the tactile qualities of different inks, papers, and binding techniques, today’s creatives are rediscovering old-fashioned printing methods and crafts. Impressive is a topical exploration of the interplay between current trends in graphic design and traditional handiwork such as letterpress printing, hand-cut linotype, chiselled woodblocks, and embossing. In addition to a rich selection of invitations, business ...

  13. Planar graphical models which are easy

    Energy Technology Data Exchange (ETDEWEB)

    Chertkov, Michael [Los Alamos National Laboratory; Chernyak, Vladimir [WAYNE STATE UNIV

    2009-01-01

    We describe a rich family of binary variables statistical mechanics models on planar graphs which are equivalent to Gaussian Grassmann Graphical models (free fermions). Calculation of partition function (weighted counting) in the models is easy (of polynomial complexity) as reduced to evaluation of determinants of matrixes linear in the number of variables. In particular, this family of models covers Holographic Algorithms of Valiant and extends on the Gauge Transformations discussed in our previous works.

  14. Graphics and statistics for cardiology: survival analysis.

    Science.gov (United States)

    May, Susanne; McKnight, Barbara

    2017-03-01

    Reports of data in the medical literature frequently lack information needed to assess the validity and generalisability of study results. Some recommendations and standards for reporting have been developed over the last two decades, but few are available specifically for survival data. We provide recommendations for tabular and graphical representations of survival data. We argue that data and analytic software should be made available to promote reproducible research.

  15. A graphical approach to analogue behavioural modelling

    OpenAIRE

    Moser, Vincent; Nussbaum, Pascal; Amann, Hans-Peter; Astier, Luc; Pellandini, Fausto

    2007-01-01

    In order to master the growing complexity of analogue electronic systems, modelling and simulation of analogue hardware at various levels is absolutely necessary. This paper presents an original modelling method based on the graphical description of analogue electronic functional blocks. This method is intended to be automated and integrated into a design framework: specialists create behavioural models of existing functional blocks, that can then be used through high-level selection and spec...

  16. Graphics-Based Parallel Programming Tools

    Science.gov (United States)

    1991-09-01

    AD-A254 406 (9 FINAL REPORT DLECTF ’AUG 13 1992 Graphics-Based Parallel Programming Tools .p Janice E. Cuny, Principal Investigator Department of...suggest parallel (either because we use a parallel graph rewriting mechanism or because we apply our results to parallel programming ), we interpret it to...was to provide support for the ex- plicit representation of graphs for use within a parallel programming environ- ment. In our environment, we view a

  17. Graphic Simulation of a Jackson Network.

    Science.gov (United States)

    1986-09-01

    Z which are algebraic combinations of other point estimates. For example, the quantity W1 is estimated using the quotient L1’/R. If at the point in...will run on IBM Compatible machines. C. TIE PROGRAMMING LANGUAGE JACKQUE is written in IBM Advanced Basic ( BASICA ). This language is provided with PC... BASICA has been loaded from DOS, and if the machine is coniigur d with a color, graphics adapter. * Printing the title screen. o Loading user defined

  18. LC/PDA/ESI-MS Profiling and Radical Scavenging Activity of Anthocyanins in Various Berries

    Directory of Open Access Journals (Sweden)

    Jun-ichiro Nakajima

    2004-01-01

    Full Text Available Anthocyanin extracts of two blueberries, Vaccinium myrtillus (bilberry and Vaccinium ashei (rabbiteye blueberry, and of three other berries, Ribes nigrum (black currant, Aronia melanocarpa (chokeberry, and Sambucus nigra (elderberry, were analyzed by high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization - mass spectrometry (LC/PDA/ESI-MS. Both bilberry and rabbiteye blueberry contained 15 identical anthocyanins with different distribution patterns. Black currant, chokeberry, and elderberry contained 6, 4, and 4 kinds of anthocyanins, respectively. The radical scavenging activities of these berry extracts were analyzed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH. All these extracts showed potent antiradical activities.

  19. Superplot: a graphical interface for plotting and analysing MultiNest output

    CERN Document Server

    Fowlie, Andrew

    2016-01-01

    We present an application, Superplot, for calculating and plotting statistical quantities relevant to parameter inference from a "chain" of samples drawn from a parameter space, produced by e.g. MultiNest. A simple graphical interface allows one to browse a chain of many variables quickly, and make publication quality plots of, inter alia, profile likelihood, posterior pdf, confidence intervals and credible regions. In this short manual, we document installation and basic usage, and define all statistical quantities and conventions.

  20. Cockpit weather graphics using mobile satellite communications

    Science.gov (United States)

    Seth, Shashi

    Many new companies are pushing state-of-the-art technology to bring a revolution in the cockpits of General Aviation (GA) aircraft. The vision, according to Dr. Bruce Holmes - the Assistant Director for Aeronautics at National Aeronautics and Space Administration's (NASA) Langley Research Center, is to provide such an advanced flight control system that the motor and cognitive skills you use to drive a car would be very similar to the ones you would use to fly an airplane. We at ViGYAN, Inc., are currently developing a system called the Pilot Weather Advisor (PWxA), which would be a part of such an advanced technology flight management system. The PWxA provides graphical depictions of weather information in the cockpit of aircraft in near real-time, through the use of broadcast satellite communications. The purpose of this system is to improve the safety and utility of GA aircraft operations. Considerable effort is being extended for research in the design of graphical weather systems, notably the works of Scanlon and Dash. The concept of providing pilots with graphical depictions of weather conditions, overlaid on geographical and navigational maps, is extremely powerful.