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Sample records for active episomal hat

  1. Antioxidant Activity/Capacity Measurement. 2. Hydrogen Atom Transfer (HAT)-Based, Mixed-Mode (Electron Transfer (ET)/HAT), and Lipid Peroxidation Assays.

    Science.gov (United States)

    Apak, Reşat; Özyürek, Mustafa; Güçlü, Kubilay; Çapanoğlu, Esra

    2016-02-10

    Measuring the antioxidant activity/capacity levels of food extracts and biological fluids is useful for determining the nutritional value of foodstuffs and for the diagnosis, treatment, and follow-up of numerous oxidative stress-related diseases. Biologically, antioxidants play their health-beneficial roles via transferring a hydrogen (H) atom or an electron (e(-)) to reactive species, thereby deactivating them. Antioxidant activity assays imitate this action; that is, antioxidants are measured by their H atom transfer (HAT) or e(-) transfer (ET) to probe molecules. Antioxidant activity/capacity can be monitored by a wide variety of assays with different mechanisms, including HAT, ET, and mixed-mode (ET/HAT) assays, generally without distinct boundaries between them. Understanding the principal mechanisms, advantages, and disadvantages of the measurement assays is important for proper selection of method for valid evaluation of antioxidant properties in desired applications. This work provides a general and up-to-date overview of HAT-based, mixed-mode (ET/HAT), and lipid peroxidation assays available for measuring antioxidant activity/capacity and the chemistry behind them, including a critical evaluation of their advantages and drawbacks.

  2. HATS-2b: A transiting extrasolar planet orbiting a K-type star showing starspot activity

    CERN Document Server

    Mohler-Fischer, M; Hartman, J D; Bakos, G B; Penev, K; Bayliss, D; Jordan, A; Csubry, Z; Zhou, G; Rabus, M; Nikolov, N; Brahm, R; Espinoza, N; Buchhave, L A; Beky, B; Suc, V; Csak, B; Henning, T; Wright, D J; Tinney, C G; Addison, B C; Schmidt, B; Noyes, R W; Papp, I; Lazar, J; Sari, P; Conroy, P

    2013-01-01

    We report the discovery of HATS-2b, the second transiting extrasolar planet detected by the HATSouth survey. HATS-2b is moving on a circular orbit around a V=13.6 mag, K-type dwarf star (GSC 6665-00236), at a separation of 0.0230 \\pm 0.0003 AU and with a period of 1.3541 days. The planetary parameters have been robustly determined using a simultaneous fit of the HATSouth, MPG/ESO~2.2\\,m/GROND, Faulkes Telescope South/Spectral transit photometry and MPG/ESO~2.2\\,m/FEROS, Euler~1.2\\,m/CORALIE, AAT~3.9\\,m/CYCLOPS radial-velocity measurements. HATS-2b has a mass of 1.37 \\pm 0.16 M_J, a radius of 1.14 \\pm 0.03 R_J and an equilibrium temperature of 1567 \\pm 30 K. The host star has a mass of 0.88 \\pm 0.04 M_Sun, radius of 0.89 \\pm 0.02 R_Sun and shows starspot activity. We characterized the stellar activity by analysing two photometric follow-up transit light curves taken with the GROND instrument, both obtained simultaneously in four optical bands (covering the wavelength range of 3860-9520 \\AA). The two light curv...

  3. Imbalance between HAT and HDAC activities in the PBMCs of patients with ankylosing spondylitis or rheumatoid arthritis and influence of HDAC inhibitors on TNF alpha production.

    Directory of Open Access Journals (Sweden)

    Eric Toussirot

    Full Text Available OBJECTIVE: Acetylation or deacetylation of histone proteins may modulate cytokine gene transcription such as TNF alpha (TNF. We evaluated the balance between histone deacetytlase (HDAC and histone acetyltransferase (HAT in patients with rheumatoid arthritis (RA or ankylosing spondylitis (AS compared to healthy controls (HC and determined the influence of HDAC inhibitors (trichostatin A -TSA- or Sirtinol -Sirt- on these enzymatic activities and on the PBMC production of TNF. METHODS: 52 patients with RA, 21 with AS and 38 HC were evaluated. HAT and HDAC activities were measured on nuclear extracts from PBMC using colorimetric assays. Enzymatic activities were determined prior to and after ex vivo treatment of PBMC by TSA or Sirt. TNF levels were evaluated in PBMC culture supernatants in the absence or presence of TSA or Sirt. RESULTS: HAT and HDAC activities were significantly reduced in AS, while these activities reached similar levels in RA and HC. Ex vivo treatment of PBMC by HDACi tended to decrease HDAC expression in HC, but Sirt significantly reduced HAT in RA. TNF production by PBMC was significantly down-regulated by Sirt in HC and AS patients. CONCLUSION: HAT and HDAC were disturbed in AS while no major changes were found in RA. HDACi may modulate HDAC and HAT PBMC expression, especially Sirt in RA. Sirtinol was able to down regulate TNF production by PBMC in HC and AS. An imbalance between HAT and HDAC activities might provide the rationale for the development of HDACi in the therapeutic approach to inflammatory rheumatic diseases.

  4. TCUP: A Novel hAT Transposon Active in Maize Tissue Culture.

    Science.gov (United States)

    Smith, Alan M; Hansey, Candice N; Kaeppler, Shawn M

    2012-01-01

    Transposable elements (TEs) are capable of inducing heritable de novo genetic variation. The sequences capable of reactivation, and environmental factors that induce mobilization, remain poorly defined even in well-studied genomes such as maize. We treated maize tissue culture with the demethylating agent 5-aza-2-deoxcytidine and examined long-term tissue culture lines to discover silenced TEs that have the potential to induce heritable genetic variation. Through these screens we have identified a novel low copy number hAT transposon, Tissue Culture Up-Regulated (TCUP), which is transcribed at high levels in long-term maize black Mexican sweet (BMS) tissue culture and is transcribed in response to treatment with 5-aza-2-deoxycytidine. Analysis of the TIGR Maize Gene Index revealed that this element is the most frequently represented EST from the BMS cell culture library and is not represented in other tissue libraries, which is the basis for its name. A full-length sequence was assembled in inbred B73 that contains the putative functional motifs required for autonomous movement of a hAT transposon. Transposon display detected novel TCUP insertions in two long-term tissue-cultured cell lines of the genotype Hi-II A × B and BMS. This research implicates TCUP as a transposon that is capable of reactivation and which may also be particularly sensitive to the stress of the tissue culture environment. Our findings are consistent with the hypothesis that epigenetic alterations potentiate genomic responses to stress during clonal propagation of plants.

  5. TCUP: A novel hAT transposon active in maize tissue culture

    Directory of Open Access Journals (Sweden)

    Alan eSmith

    2012-01-01

    Full Text Available Transposable elements are capable of inducing heritable de novo genetic variation. The sequences capable of reactivation, and environmental factors that induce mobilization, remain poorly defined even in well-studied genomes such as maize. We treated maize tissue culture with the demethylating agent 5-aza-2-deoxcytidine and examined long-term tissue culture lines to discover silenced transposable elements that have the potential to induce heritable genetic variation. Through these screens we have identified a novel low copy number hAT transposon, Tissue Culture Up-Regulated (TCUP, which is transcribed at high levels in long-term maize Black Mexican Sweet (BMS tissue culture and up-regulated in response to treatment with 5-aza-2-deoxycytidine. Analysis of the TIGR Maize Gene Index revealed that this element is the most frequently represented EST from the BMS cell culture library and is not represented in other tissue libraries, which is the basis for its name. A full-length sequence was assembled in inbred B73 that contains the putative functional motifs required for autonomous movement of a hAT transposon. Transposon display detected movement of TCUP in two long-term tissue cultured cell lines of the genotype Hi-II AxB and BMS. This research implicates TCUP as a transposon that is capable of reactivation and which may also be particularly sensitive to the stress of the tissue culture environment. Our findings are consistent with the hypothesis that epigenetic alterations potentiate genomic responses to stress during clonal propagation of plants.

  6. An active hAT transposable element causing bud mutation of carnation by insertion into the flavonoid 3'-hydroxylase gene.

    Science.gov (United States)

    Momose, Masaki; Nakayama, Masayoshi; Itoh, Yoshio; Umemoto, Naoyuki; Toguri, Toshihiro; Ozeki, Yoshihiro

    2013-04-01

    The molecular mechanisms underlying spontaneous bud mutations, which provide an important breeding tool in carnation, are poorly understood. Here we describe a new active hAT type transposable element, designated Tdic101, the movement of which caused a bud mutation in carnation that led to a change of flower color from purple to deep pink. The color change was attributed to Tdic101 insertion into the second intron of F3'H, the gene for flavonoid 3'-hydroxylase responsible for purple pigment production. Regions on the deep pink flowers of the mutant can revert to purple, a visible phenotype of, as we show, excision of the transposable element. Sequence analysis revealed that Tdic101 has the characteristics of an autonomous element encoding a transposase. A related, but non-autonomous element dTdic102 was found to move in the genome of the bud mutant as well. Its mobilization might be the result of transposase activities provided by other elements such as Tdic101. In carnation, therefore, the movement of transposable elements plays an important role in the emergence of a bud mutation.

  7. The Three Hat Problem

    CERN Document Server

    Benson, Brian

    2007-01-01

    In this paper we study the Three Hat Problem which appeared in Puzzle Corner of the Technology Review magazine. This puzzle gives a scenario in which three players wearing hats are sitting together and each hat can be seen by everyone except the player that is wearing that hat. Each player is told that all of the hats contain a positive integer and that two of the integers add to the third. In an ordered, turn-wise, modular fashion, each player truthfully states whether or not he knows his integer. We give a strategy which allows for one of the players to solve for his integer for all possible integer configurations of the puzzle and prove it is the optimal such strategy.

  8. Quiescent and proliferative fibroblasts exhibit differential p300 HAT activation through control of 5-methoxytryptophan production.

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    Huei-Hsuan Cheng

    Full Text Available Quiescent fibroblasts possess unique genetic program and exhibit high metabolic activity distinct from proliferative fibroblasts. In response to inflammatory stimulation, quiescent fibroblasts are more active in expressing cyclooxygenase-2 and other proinflammatory genes than proliferative fibroblasts. The underlying transcriptional mechanism is unclear. Here we show that phorbol 12-myristate 13-acetate (PMA and cytokines increased p300 histone acetyltransferase activity to a higher magnitude (> 2 fold in quiescent fibroblasts than in proliferative fibroblasts. Binding of p300 to cyclooxygenase-2 promoter was reduced in proliferative fibroblasts. By ultrahigh-performance liquid chromatography coupled with a quadrupole time of flight mass spectrometer and enzyme-immunoassay, we found that production of 5-methoxytryptophan was 2-3 folds higher in proliferative fibroblasts than that in quiescent fibroblasts. Addition of 5-methoxytryptophan and its metabolic precursor, 5-hydroxytryptophan, to quiescent fibroblasts suppressed PMA-induced p300 histone acetyltransferase activity and cyclooxygenase-2 expression to the level of proliferative fibroblasts. Silencing of tryptophan hydroxylase-1 or hydroxyindole O-methyltransferase in proliferative fibroblasts with siRNA resulted in elevation of PMA-induced p300 histone acetyltransferase activity to the level of that in quiescent fibroblasts, which was rescued by addition of 5-hydroxytryptophan or 5-methoxytryptophan. Our findings indicate that robust inflammatory gene expression in quiescent fibroblasts vs. proliferative fibroblasts is attributed to uncontrolled p300 histone acetyltransferase activation due to deficiency of 5-methoxytryptophan production. 5-methoxytryptophan thus is a potential valuable lead compound for new anti-inflammatory drug development.

  9. Association with the origin recognition complex suggests a novel role for histone acetyltransferase Hat1p/Hat2p

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    Greenblatt Jack F

    2007-09-01

    Full Text Available Abstract Background Histone modifications have been implicated in the regulation of transcription and, more recently, in DNA replication and repair. In yeast, a major conserved histone acetyltransferase, Hat1p, preferentially acetylates lysine residues 5 and 12 on histone H4. Results Here, we report that a nuclear sub-complex consisting of Hat1p and its partner Hat2p interacts physically and functionally with the origin recognition complex (ORC. While mutational inactivation of the histone acetyltransferase (HAT gene HAT1 alone does not compromise origin firing or initiation of DNA replication, a deletion in HAT1 (or HAT2 exacerbates the growth defects of conditional orc-ts mutants. Thus, the ORC-associated Hat1p-dependent histone acetyltransferase activity suggests a novel linkage between histone modification and DNA replication. Additional genetic and biochemical evidence points to the existence of partly overlapping histone H3 acetyltransferase activities in addition to Hat1p/Hat2p for proper DNA replication efficiency. Furthermore, we demonstrated a dynamic association of Hat1p with chromatin during S-phase that suggests a role of this enzyme at the replication fork. Conclusion We have found an intriguing new association of the Hat1p-dependent histone acetyltransferase in addition to its previously known role in nuclear chromatin assembly (Hat1p/Hat2p-Hif1p. The participation of a distinct Hat1p/Hat2p sub-complex suggests a linkage of histone H4 modification with ORC-dependent DNA replication.

  10. Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts.

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    Stephanie Bertram

    Full Text Available The type II transmembrane serine proteases TMPRSS2 and HAT activate influenza viruses and the SARS-coronavirus (TMPRSS2 in cell culture and may play an important role in viral spread and pathogenesis in the infected host. However, it is at present largely unclear to what extent these proteases are expressed in viral target cells in human tissues. Here, we show that both HAT and TMPRSS2 are coexpressed with 2,6-linked sialic acids, the major receptor determinant of human influenza viruses, throughout the human respiratory tract. Similarly, coexpression of ACE2, the SARS-coronavirus receptor, and TMPRSS2 was frequently found in the upper and lower aerodigestive tract, with the exception of the vocal folds, epiglottis and trachea. Finally, activation of influenza virus was conserved between human, avian and porcine TMPRSS2, suggesting that this protease might activate influenza virus in reservoir-, intermediate- and human hosts. In sum, our results show that TMPRSS2 and HAT are expressed by important influenza and SARS-coronavirus target cells and could thus support viral spread in the human host.

  11. MAR characteristic motifs mediate episomal vector in CHO cells.

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    Lin, Yan; Li, Zhaoxi; Wang, Tianyun; Wang, Xiaoyin; Wang, Li; Dong, Weihua; Jing, Changqin; Yang, Xianjun

    2015-04-01

    An ideal gene therapy vector should enable persistent transgene expression without limitations in safety and reproducibility. Recent researches' insight into the ability of chromosomal matrix attachment regions (MARs) to mediate episomal maintenance of genetic elements allowed the development of a circular episomal vector. Although a MAR-mediated engineered vector has been developed, little is known on which motifs of MAR confer this function during interaction with the host genome. Here, we report an artificially synthesized DNA fragment containing only characteristic motif sequences that served as an alternative to human beta-interferon matrix attachment region sequence. The potential of the vector to mediate gene transfer in CHO cells was investigated. The short synthetic MAR motifs were found to mediate episomal vector at a low copy number for many generations without integration into the host genome. Higher transgene expression was maintained for at least 4 months. In addition, MAR was maintained episomally and conferred sustained EGFP expression even in nonselective CHO cells. All the results demonstrated that MAR characteristic sequence-based vector can function as stable episomes in CHO cells, supporting long-term and effective transgene expression.

  12. Hard-hat day

    CERN Multimedia

    2003-01-01

    CERN will be organizing a special information day on Friday, 27th June, designed to promote the wearing of hard hats and ensure that they are worn correctly. A new prevention campaign will also be launched.The event will take place in the hall of the Main Building from 11.30 a.m. to 2.00 p.m., when you will be able to come and try on various models of hard hat, including some of the very latest innovative designs, ask questions and pass on any comments and suggestions.

  13. Program Transformation in HATS

    Energy Technology Data Exchange (ETDEWEB)

    Winter, V.L.

    1999-02-24

    HATS is a general purpose syntax derivation tree based transformation system in which transformation sequences are described in special purpose language. A powerful feature of this language is that unification is an explicit operation. By making unification explicit, an elegant framework arises in which to express complex application conditions which in turn enables refined control strategies to be realized. This paper gives an overview of HATS, focusing especially on the framework provided by the transformation language and its potential with respect to control and general purpose transformation.

  14. Chromatin Structure of Epstein-Barr Virus Latent Episomes.

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    Lieberman, Paul M

    2015-01-01

    EBV latent infection is characterized by a highly restricted pattern of viral gene expression. EBV can establish latent infections in multiple different tissue types with remarkable variation and plasticity in viral transcription and replication. During latency, the viral genome persists as a multi-copy episome, a non-integrated-closed circular DNA with nucleosome structure similar to cellular chromosomes. Chromatin assembly and histone modifications contribute to the regulation of viral gene expression, DNA replication, and episome persistence during latency. This review focuses on how EBV latency is regulated by chromatin and its associated processes.

  15. The HAT TRICK programme for improving physical activity, healthy eating and connectedness among overweight, inactive men: study protocol of a pragmatic feasibility trial.

    Science.gov (United States)

    Caperchione, Cristina M; Bottorff, Joan L; Oliffe, John L; Johnson, Steven T; Hunt, Kate; Sharp, Paul; Fitzpatrick, Kayla M; Price, Ryley; Goldenberg, S Larry

    2017-09-06

    Physical activity, healthy eating and maintaining a healthy weight are associated with reduced risk of cardiovascular disease, type 2 diabetes and cancer and with improved mental health. Despite these benefits, many men do not meet recommended physical activity guidelines and have poor eating behaviours. Many health promotion programmes hold little appeal to men and consequently fail to influence men's health practices. HAT TRICK was designed as a 12-week face-to-face, gender-sensitised intervention for overweight and inactive men focusing on physical activity, healthy eating and social connectedness and was delivered in collaboration with a major junior Canadian ice hockey team (age range 16-20 years). The programme was implemented and evaluated to assess its feasibility. This article describes the intervention design and study protocol of HAT TRICK. HAT TRICK participants (n=60) were men age 35 years, residing in the Okanagan Region of British Columbia, who accumulate 150 min of moderate to vigorous physical activity a week, with a body mass index of >25 kg/m(2) and a pant waist size of >38'. Each 90 min weekly session included targeted health education and theory-guided behavioural change techniques, as well as a progressive (ie, an increase in duration and intensity) group physical activity component. Outcome measures were collected at baseline, 12 weeks and 9 months and included the following: objectively measured anthropometrics, blood pressure, heart rate, physical activity and sedentary behaviour, as well as self-reported physical activity, sedentary behaviour, diet, smoking, alcohol consumption, sleep habits, risk of depression, health-related quality of life and social connectedness. Programme feasibility data (eg, recruitment, satisfaction, adherence, content delivery) were assessed at 12 weeks via interviews and self-report. Ethical approval was obtained from the University of British Columbia Okanagan Behavioural Research Ethics Board (reference no H

  16. Rodent under the hat.

    Science.gov (United States)

    Lo, William B; Richmond, Lewys; George, K Joshi; Dardis, Ronan

    2011-10-01

    This report describes a case of neglected scalp basal cell carcinoma (BCC) resulting in total destruction of soft tissue and underlying cranium, with remarkably preserved dura and no parenchymal involvement. A 57-year-old woman presented with a 2-week history of lethargy. On removal of her hat, a large round ulcer was revealed. It transpired that the patient noticed a pruritic scalp lesion 9 months ago. As the lesion and wound enlarged, she was too embarrassed to inform her family and hid it under a hat at all time. She never experienced meningitic symptoms. Biopsy confirmed BCC. Subsequently, she underwent two free latissimus dorsi flap reconstructions to cover the wound and palliative radiotherapy. She responded well to treatment. This case provides a rare opportunity to observe the natural history of scalp BCC. The reasons for the rarity of this mode of presentation and the low incidence of intracranial infection are discussed.

  17. Monitoring glaciers and indications of subglacial volcanic activity using small-scale Top-Hat reflectors - An IsViews experiment on Myrdalsjökull, Iceland

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    Minet, Christian; Duque Biarge, Sergi; Jaenicke, Julia; Münzer, Ulrich; Mayer, Christoph; Franke, Jonas; Guðmundsson, Águst; Parizzi, Alessandro; Fritz, Thomas; Eineder, Michael

    2014-05-01

    Subglacial volcanic eruptions often provide indications of activity some time before the actual catastrophic event. Surface undulations appear on top of the ice cap and meltwater torrents can occur at the glacier margin. Even large scale uplifts of ice caps have been observed. Within the project IsViews a processing chain, based on high spatially and temporally resolved remote sensing imagery, will be developed in order to automatically identify such early indications. The main data used for this analysis are acquired by the TerraSAR-X, TanDEM-X and RapidEye satellites. First investigations concerning the feasibility of the near real-time warning system and the general baseline conditions are carried out on two large plateau glaciers in southern Iceland, namely Mördalsjökull and Vatnajökull. Within the 2013 IsViews field work an experiment was started in order to test a new way of glacier monitoring. Two test sites were established on the Mördalsjökull ice cap (one at the equilibrium line and one below), each consisting of a permanent GPS station and two nearby RADAR reflectors. These RADAR reflectors are specially designed Top-Hat reflectors, which are cheap to manufacture, small (50 cm diameter) and lightweight and therefore easy to handle, transport and deploy. Their special design makes them visible in SAR images independent of orientation, so different acquisition geometries and even different sensors can be used. The drawback of the small, low reflecting Top-Hat can be overcome by using the newly implemented Staring Spotlight Mode of the German SAR Satellite TerraSAR-X, providing an unprecedented resolution of down to 20 cm in the azimuth direction. The reflectors, as point targets, allow absolute positioning within the cm-level in the TerraSAR-X data. Time series of SAR data can be used to derive position and altitude changes of the reflector itself and possibly even melting rates by exploiting the different signal paths. The visibility of the Top-Hat

  18. Glucocorticoid regulation of transcription at an amplified, episomal promoter

    Energy Technology Data Exchange (ETDEWEB)

    Ostrowski, M.C.; Richard-Foy, H.; Wolford, R.G.; Berard, D.S.; Hager, G.L.

    1983-11-01

    The mouse mammary tumor virus long terminal repeat (MMTV LTR) has been introduced into cultured murine cells, using the 69% transforming fragment of bovine papiloma virus type 1 (BVP). Transformed cells contain up to 200 copies of the chimeric molecules per diploid genome. The restriction endonuclease map of the acquired recombinants, as well as the physical structure of the DNA, indicates that the LTR-BVP molecules present in these cells occur exclusively as unintegrated, extrachromosomal episome. When a 72-base pair direct repeat ''enhancer'' element (derived from the Harvey sarcoma retrovirus) was included in the MMTV LTR-BPV chimeric plasmids, DNA acquired through transfection, with a single exception, was integrated or rearranged or both. Two approaches showed that the MMTV LTR present in the episomal state was capable of supporting glucocorticoid hormone-regulated transcription. The authors have therefore demonstrated the hormone response for the first time in a totally defined primary sequence environment. Significant differences both in the basal level of MMTV-initiated transcription and in the extend of glucocorticoid induction were observed in individual cell lines with similar episomal copy numbers. These phenotypic variations suggest that epigenetic structure is an important component of the mechanism of regulation.

  19. HATS-15 b and HATS-16 b: Two massive planets transiting old G dwarf stars

    CERN Document Server

    Ciceri, S; Henning, T; Bakos, G Á; Penev, K; Brahm, R; Zhou, G; Hartman, J D; Bayliss, D; Jordán, A; Csubry, Z; de Val-Borro, M; Bhatti, W; Rabus, M; Espinoza, N; Suc, V; Schmidt, B; Noyes, R; Howard, A W; Fulton, B J; Isaacson, H; Marcy, G W; Butler, R P; Arriagada, P; Crane, J; Shectman, S; Thompson, I; Tan, T G; Lázár, J; Papp, I; Sari, P

    2015-01-01

    We report the discovery of HATS-15 b and HATS-16 b, two massive transiting extrasolar planets orbiting evolved ($\\sim 10$ Gyr) main-sequence stars. The planet HATS-15 b, which is hosted by a G9V star ($V=14.8$ mag), is a hot Jupiter with mass of $2.17\\pm0.15\\, M_{\\mathrm{J}}$ and radius of $1.105\\pm0.0.040\\, R_{\\mathrm{J}}$, and completes its orbit in nearly 1.7 days. HATS-16 b is a very massive hot Jupiter with mass of $3.27\\pm0.19\\, M_{\\mathrm{J}}$ and radius of $1.30\\pm0.15\\, R_{\\mathrm{J}}$; it orbits around its G3 V parent star ($V=13.8$ mag) in $\\sim2.7$ days. HATS-16 is slightly active and shows a periodic photometric modulation, implying a rotational period of 12 days which is unexpectedly short given its isochronal age. This fast rotation might be the result of the tidal interaction between the star and its planet.

  20. Dissecting the Molecular Roles of Histone Chaperones in Histone Acetylation by Type B Histone Acetyltransferases (HAT-B).

    Science.gov (United States)

    Haigney, Allison; Ricketts, M Daniel; Marmorstein, Ronen

    2015-12-18

    The HAT-B enzyme complex is responsible for acetylating newly synthesized histone H4 on lysines K5 and K12. HAT-B is a multisubunit complex composed of the histone acetyltransferase 1 (Hat1) catalytic subunit and the Hat2 (rbap46) histone chaperone. Hat1 is predominantly localized in the nucleus as a member of a trimeric NuB4 complex containing Hat1, Hat2, and a histone H3-H4 specific histone chaperone called Hif1 (NASP). In addition to Hif1 and Hat2, Hat1 interacts with Asf1 (anti-silencing function 1), a histone chaperone that has been reported to be involved in both replication-dependent and -independent chromatin assembly. To elucidate the molecular roles of the Hif1 and Asf1 histone chaperones in HAT-B histone binding and acetyltransferase activity, we have characterized the stoichiometry and binding mode of Hif1 and Asf1 to HAT-B and the effect of this binding on the enzymatic activity of HAT-B. We find that Hif1 and Asf1 bind through different modes and independently to HAT-B, whereby Hif1 binds directly to Hat2, and Asf1 is only capable of interactions with HAT-B through contacts with histones H3-H4. We also demonstrate that HAT-B is significantly more active against an intact H3-H4 heterodimer over a histone H4 peptide, independent of either Hif1 or Asf1 binding. Mutational studies further demonstrate that HAT-B binding to the histone tail regions is not sufficient for this enhanced activity. Based on these data, we propose a model for HAT-B/histone chaperone assembly and acetylation of H3-H4 complexes.

  1. Evolutionary origin of Rosaceae-specific active non-autonomous hAT elements and their contribution to gene regulation and genomic structural variation.

    Science.gov (United States)

    Wang, Lu; Peng, Qian; Zhao, Jianbo; Ren, Fei; Zhou, Hui; Wang, Wei; Liao, Liao; Owiti, Albert; Jiang, Quan; Han, Yuepeng

    2016-05-01

    Transposable elements account for approximately 30 % of the Prunus genome; however, their evolutionary origin and functionality remain largely unclear. In this study, we identified a hAT transposon family, termed Moshan, in Prunus. The Moshan elements consist of three types, aMoshan, tMoshan, and mMoshan. The aMoshan and tMoshan types contain intact or truncated transposase genes, respectively, while the mMoshan type is miniature inverted-repeat transposable element (MITE). The Moshan transposons are unique to Rosaceae, and the copy numbers of different Moshan types are significantly correlated. Sequence homology analysis reveals that the mMoshan MITEs are direct deletion derivatives of the tMoshan progenitors, and one kind of mMoshan containing a MuDR-derived fragment were amplified predominately in the peach genome. The mMoshan sequences contain cis-regulatory elements that can enhance gene expression up to 100-fold. The mMoshan MITEs can serve as potential sources of micro and long noncoding RNAs. Whole-genome re-sequencing analysis indicates that mMoshan elements are highly active, and an insertion into S-haplotype-specific F-box gene was reported to cause the breakdown of self-incompatibility in sour cherry. Taken together, all these results suggest that the mMoshan elements play important roles in regulating gene expression and driving genomic structural variation in Prunus.

  2. DIY teleport hats - the consolation of technology

    DEFF Research Database (Denmark)

    Hansen, Lone Koefoed

    2009-01-01

    This instructable describes how to make a set of teleport hats. A teleport hat is a crocheted hat which doubles as a device with which you can teleport yourself to a person wearing the same type of hat in case you miss oneanother and want to be together without the hassle of driving, jetting...

  3. Efficient feeder-free episomal reprogramming with small molecules.

    Directory of Open Access Journals (Sweden)

    Junying Yu

    Full Text Available Genetic reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs could offer replenishable cell sources for transplantation therapies. To fulfill their promises, human iPSCs will ideally be free of exogenous DNA (footprint-free, and be derived and cultured in chemically defined media free of feeder cells. Currently, methods are available to enable efficient derivation of footprint-free human iPSCs. However, each of these methods has its limitations. We have previously derived footprint-free human iPSCs by employing episomal vectors for transgene delivery, but the process was inefficient and required feeder cells. Here, we have greatly improved the episomal reprogramming efficiency using a cocktail containing MEK inhibitor PD0325901, GSK3β inhibitor CHIR99021, TGF-β/Activin/Nodal receptor inhibitor A-83-01, ROCK inhibitor HA-100 and human leukemia inhibitory factor. Moreover, we have successfully established a feeder-free reprogramming condition using chemically defined medium with bFGF and N2B27 supplements and chemically defined human ESC medium mTeSR1 for the derivation of footprint-free human iPSCs. These improvements enabled the routine derivation of footprint-free human iPSCs from skin fibroblasts, adipose tissue-derived cells and cord blood cells. This technology will likely be valuable for the production of clinical-grade human iPSCs.

  4. Episomal and integrated maintenance of foreign DNA in Giardia lamblia.

    Science.gov (United States)

    Singer, S M; Yee, J; Nash, T E

    1998-04-01

    Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltransferase (pac) as a dominant selectable marker. Transfected circular DNAs were maintained as episomes in the isolate WB, a representative of one Giardia subgroup. When input DNAs were linearized, integration was observed to occur by homologous recombination producing gene replacements in this isolate. In isolate GS, which represents a different subgroup, both linear and circular transfected DNAs were integrated into the genome by homologous recombination. In GS, linear DNA again produced gene replacements, while circular DNA produced duplicative integration events. The failure of GS to replicate episomes may reflect differences in the structure or recognition of DNA replication origins between these subgroups. A plasmid shuttle vector was also developed for expression of other genes in Giardia lamblia. Utilizing the green fluorescent protein as a reporter gene in the WB isolate, we show that gene expression from this vector correlated with plasmid copy number over a range of two orders of magnitude. Together these tools should greatly enhance our ability to study both the basic biology and the pathogenesis of this ubiquitous parasite.

  5. AKT1 loss correlates with episomal HPV16 in vulval intraepithelial neoplasia.

    Directory of Open Access Journals (Sweden)

    Arucha L Ekeowa-Anderson

    Full Text Available Anogenital malignancy has a significant association with high-risk mucosal alpha-human papillomaviruses (alpha-PV, particularly HPV 16 and 18 whereas extragenital SCC has been linked to the presence of cutaneous beta and gamma-HPV types. Vulval skin may be colonised by both mucosal and cutaneous (beta-, mu-, nu- and gamma- PV types, but there are few systematic studies investigating their presence and their relative contributions to vulval malignancy. Dysregulation of AKT, a serine/threonine kinase, plays a significant role in several cancers. Mucosal HPV types can increase AKT phosphorylation and activity whereas cutaneous HPV types down-regulate AKT1 expression, probably to weaken the cornified envelope to promote viral release. We assessed the presence of mucosal and cutaneous HPV in vulval malignancy and its relationship to AKT1 expression in order to establish the corresponding HPV and AKT1 profile of normal vulval skin, vulval intraepithelial neoplasia (VIN and vulval squamous cell carcinoma (vSCC. We show that HPV16 is the principle HPV type present in VIN, there were few detectable beta types present and AKT1 loss was not associated with the presence of these cutaneous HPV. We show that HPV16 early gene expression reduced AKT1 expression in transgenic mouse epidermis. AKT1 loss in our VIN cohort correlated with presence of high copy number, episomal HPV16. Maintained AKT1 expression correlated with low copy number, an increased frequency of integration and increased HPV16E7 expression, a finding we replicated in another untyped cohort of vSCC. Since expression of E7 reflects tumour progression, these findings suggest that AKT1 loss associated with episomal HPV16 may have positive prognostic implications in vulval malignancy.

  6. DIY teleport hats - the consolation of technology

    DEFF Research Database (Denmark)

    Hansen, Lone Koefoed

    2009-01-01

    This instructable describes how to make a set of teleport hats. A teleport hat is a crocheted hat which doubles as a device with which you can teleport yourself to a person wearing the same type of hat in case you miss oneanother and want to be together without the hassle of driving, jetting or b...... but it works well as a gift for someone you miss or someone who misses another person madly as it provides an opportunity to take some kind of action when ordinary options like driving, jetting or biking are not feasible even if the action is only symbolic.......This instructable describes how to make a set of teleport hats. A teleport hat is a crocheted hat which doubles as a device with which you can teleport yourself to a person wearing the same type of hat in case you miss oneanother and want to be together without the hassle of driving, jetting...

  7. Multiple roles for acetylation in the interaction of p300 HAT with ATF-2.

    Science.gov (United States)

    Karanam, Balasubramanyam; Wang, Ling; Wang, Dongxia; Liu, Xin; Marmorstein, Ronen; Cotter, Robert; Cole, Philip A

    2007-07-17

    The transcriptional coactivator paralogues p300 and CBP contain acetyltransferase domains (HAT) and catalyze the lysine acetylation of histones and other proteins as an important aspect of their functions. Prior studies revealed that the basic leucine zipper domain (b-ZIP) of transcription factor ATF-2 (also called CRE-BP1) can interact with the CBP HAT domain. In this study, we have examined the ATF-2 b-ZIP interaction with the p300 HAT domain and shown that p300 HAT autoacetylation can enhance the binding affinity. Pull-down assays revealed that hyperacetylated p300 HAT is more efficiently retained by immobilized ATF-2 b-ZIP than hypoacetylated p300 HAT. Loop deleted p300 HAT lacking autoacetylation was retained about as well as hyperacetylated p300 HAT, suggesting that the loop and ATF-2 compete for p300 HAT binding. While ATF-2 b-ZIP is a weak inhibitor of hypoacetylated p300 HAT acetylation of a histone H4 peptide, hyperacetylated p300 HAT is much more potently inhibited by ATF-2 b-ZIP. Moreover, we showed that ATF-2 b-ZIP could serve as an acetyltransferase substrate for p300 HAT. Using mass spectrometry, two p300 HAT lysine acetylation sites were mapped in ATF-2 b-ZIP. Immunoprecipitation-Western blot analysis with anti-acetyl-lysine antibody revealed that ATF-2 can undergo reversible acetylation in vivo. Mutational analysis of the two ATF-2 b-ZIP acetylation sites revealed their potential contributions to ATF-2-mediated transcriptional activation. Taken together, these studies suggest multiple roles for protein acetylation in the regulation of transcription by p300/CBP and ATF-2.

  8. Molecular basis for oligomeric-DNA binding and episome maintenance by KSHV LANA.

    Directory of Open Access Journals (Sweden)

    John F Domsic

    Full Text Available LANA is the KSHV-encoded terminal repeat binding protein essential for viral replication and episome maintenance during latency. We have determined the X-ray crystal structure of LANA C-terminal DNA binding domain (LANADBD to reveal its capacity to form a decameric ring with an exterior DNA binding surface. The dimeric core is structurally similar to EBV EBNA1 with an N-terminal arm that regulates DNA binding and is required for replication function. The oligomeric interface between LANA dimers is dispensable for single site DNA binding, but is required for cooperative DNA binding, replication function, and episome maintenance. We also identify a basic patch opposite of the DNA binding surface that is responsible for the interaction with BRD proteins and contributes to episome maintenance function. The structural features of LANADBD suggest a novel mechanism of episome maintenance through DNA-binding induced oligomeric assembly.

  9. The stealth episome: suppression of gene expression on the excised genomic island PPHGI-1 from Pseudomonas syringae pv. phaseolicola.

    Directory of Open Access Journals (Sweden)

    Scott A C Godfrey

    2011-03-01

    Full Text Available Pseudomonas syringae pv. phaseolicola is the causative agent of halo blight in the common bean, Phaseolus vulgaris. P. syringae pv. phaseolicola race 4 strain 1302A contains the avirulence gene avrPphB (syn. hopAR1, which resides on PPHGI-1, a 106 kb genomic island. Loss of PPHGI-1 from P. syringae pv. phaseolicola 1302A following exposure to the hypersensitive resistance response (HR leads to the evolution of strains with altered virulence. Here we have used fluorescent protein reporter systems to gain insight into the mobility of PPHGI-1. Confocal imaging of dual-labelled P. syringae pv. phaseolicola 1302A strain, F532 (dsRFP in chromosome and eGFP in PPHGI-1, revealed loss of PPHGI-1::eGFP encoded fluorescence during plant infection and when grown in vitro on extracted leaf apoplastic fluids. Fluorescence-activated cell sorting (FACS of fluorescent and non-fluorescent PPHGI-1::eGFP F532 populations showed that cells lost fluorescence not only when the GI was deleted, but also when it had excised and was present as a circular episome. In addition to reduced expression of eGFP, quantitative PCR on sub-populations separated by FACS showed that transcription of other genes on PPHGI-1 (avrPphB and xerC was also greatly reduced in F532 cells harbouring the excised PPHGI-1::eGFP episome. Our results show how virulence determinants located on mobile pathogenicity islands may be hidden from detection by host surveillance systems through the suppression of gene expression in the episomal state.

  10. Differential methylation of E2 binding sites in episomal and integrated HPV 16 genomes in preinvasive and invasive cervical lesions.

    Science.gov (United States)

    Chaiwongkot, Arkom; Vinokurova, Svetlana; Pientong, Chamsai; Ekalaksananan, Tipaya; Kongyingyoes, Bunkerd; Kleebkaow, Pilaiwan; Chumworathayi, Bandit; Patarapadungkit, Natcha; Reuschenbach, Miriam; von Knebel Doeberitz, Magnus

    2013-05-01

    Enhanced expression of the HPV 16 E6-E7 oncogenes may trigger neoplastic transformation of the squamous epithelial cells at the uterine cervix. The HPV E2 protein is a key transcriptional regulator of the E6-E7 genes. It binds to four E2 binding sites (E2BSs 1-4) in the viral upstream regulatory region (URR). Modification of E2 functions, for example, by methylation of E2BSs is hypothesized to trigger enhanced expression of the viral E6-E7 oncogenes. In the majority of HPV-transformed premalignant lesions and about half of cervical carcinomas HPV genomes persist in an extra-chromosomal, episomal state, whereas they are integrated into host cells chromosomes in the remaining lesions. Here we compared the methylation profile of E2BSs 1-4 of the HPV 16 URR in a series of 18 HPV16-positive premalignant lesions and 33 invasive cervical cancers. CpGs within the E2BSs 1, 3, and 4 were higher methylated in all lesions with only episomal HPV16 genomes compared with lesions displaying single integrated copies. Samples with multiple HPV16 integrated copies displayed high methylation levels for all CpGs suggesting that the majority of multiple copies were silenced by extensive methylation. These data support the hypothesis that differential methylation of the E2BSs 1, 3 and 4 is related to the activation of viral oncogene expression in cervical lesions as long as the viral genome remains in the episomal state. Once the virus becomes integrated into host cell chromosomes these methylation patterns may be substantially altered due to complex epigenetic changes of integrated HPV genomes.

  11. Cloned origin of DNA replication in c-myc gene can function and be transmitted in transgenic mice in an episomal state.

    OpenAIRE

    Sudo, Katsuko; Ogata, Masaaki; Sato, Yoshinari; Iguchi-Ariga, Sanae M. M.; Ariga, Hiroyoshi

    1990-01-01

    The c-myc protein has recently been shown to interact with a region possessing putative origin of DNA replication and enhancer activities located 2 kb upstream of the c-myc gene itself. Transgenic mice were obtained by injecting constructs containing this region, termed pmyc(H-P), into fertilized mouse eggs. The transgenic elements were capable of efficient replication in all mouse tissues examined and were maintained in an episomal state even in highly differentiated cells. Moreover, pmyc(H-...

  12. Woman Rider with Veiled Hat

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    In ancient times, women who went outside had to cover their faces so that passersby would not see them. During the early days of the Tang Dynasty, women wore veiled hats with semi-transparent gauze covering their faces. The veiled hat was developed by the northwestern nationalities who wore them to protect their faces from wind and dust. But the Han women wore them to cover their faces. This yellow-glazed piece is 36.6cm high. The sturdy horse stands with shining eyes, cropped mane and tail. The rider wears a round, collarless, narrow-sleeved garment and high-waisted long skirt. She has a long nose and wide eyes and

  13. HAT-P-38h

    DEFF Research Database (Denmark)

    Sato, Bun'ei; Hartman, Joel D.; Bakos, Gaspar A.

    2012-01-01

    We report on the discovery of HAT-P-38b, a Saturn-mass exoplanet, transiting the V = 12.56 dwarf star GSC 2314-00559 on a P = 4.6404 d circular orbit. The host star is a 0.89 M-circle dot late G dwarf, with solar metallicity and a radius of 0.92 R-circle dot. The planetary companion has a mass of 0.......27 M-J and a radius of 0.82 R-J. HAT-P-38b is one of the planets the mass and radius of which have ever been discovered to be the closest to those of Saturn....

  14. HAT-P-16b

    DEFF Research Database (Denmark)

    Buchhave, Lars A.; Bakos, G. A.; Hartman, J. D.

    2010-01-01

    .04 M , radius of 1.24 ± 0.05 R , effective temperature 6158 ± 80 K, and metallicity [Fe/H] = +0.17 ± 0.08. The planetary companion has a mass of 4.193 ± 0.094 M J and radius of 1.289 ± 0.066 R J, yielding a mean density of 2.42 ± 0.35 g cm–3. Comparing these observed characteristics with recent...... theoretical models, we find that HAT-P-16b is consistent with a 1 Gyr H/He-dominated gas giant planet. HAT-P-16b resides in a sparsely populated region of the mass-radius diagram and has a non-zero eccentricity of e = 0.036 with a significance of 10s....

  15. HAT-P-25b

    DEFF Research Database (Denmark)

    Quinn, S.N.; Bakos, G. 'A; Hartman, J.

    2012-01-01

    We report the discovery of HAT-P-25b, a transiting extrasolar planet orbiting the V = 13.19 G5 dwarf star GSC 1788-01237, with a period P = 3.652836 +/- 0.000019 days, transit epoch Tc = 2455176.85173 +/- 0.00047 (BJD), and transit duration 0.1174 +/- 0.0017 days. The host star has mass of 1.01 +...

  16. HAT-P-38h

    DEFF Research Database (Denmark)

    Sato, Bun'ei; Hartman, Joel D.; Bakos, Gaspar A.

    2012-01-01

    We report on the discovery of HAT-P-38b, a Saturn-mass exoplanet, transiting the V = 12.56 dwarf star GSC 2314-00559 on a P = 4.6404 d circular orbit. The host star is a 0.89 M-circle dot late G dwarf, with solar metallicity and a radius of 0.92 R-circle dot. The planetary companion has a mass of 0...

  17. HAT-P-38h

    DEFF Research Database (Denmark)

    Sato, Bun'ei; Hartman, Joel D.; Bakos, Gaspar Á.

    2012-01-01

    We report on the discovery of HAT-P-38b, a Saturn-mass exoplanet, transiting the V = 12.56 dwarf star GSC 2314-00559 on a P = 4.6404 d circular orbit. The host star is a 0.89 M-circle dot late G dwarf, with solar metallicity and a radius of 0.92 R-circle dot. The planetary companion has a mass of...

  18. Plasticity of DNA replication initiation in Epstein-Barr virus episomes.

    Directory of Open Access Journals (Sweden)

    Paolo Norio

    2004-06-01

    Full Text Available In mammalian cells, the activity of the sites of initiation of DNA replication appears to be influenced epigenetically, but this regulation is not fully understood. Most studies of DNA replication have focused on the activity of individual initiation sites, making it difficult to evaluate the impact of changes in initiation activity on the replication of entire genomic loci. Here, we used single molecule analysis of replicated DNA (SMARD to study the latent duplication of Epstein-Barr virus (EBV episomes in human cell lines. We found that initiation sites are present throughout the EBV genome and that their utilization is not conserved in different EBV strains. In addition, SMARD shows that modifications in the utilization of multiple initiation sites occur across large genomic regions (tens of kilobases in size. These observations indicate that individual initiation sites play a limited role in determining the replication dynamics of the EBV genome. Long-range mechanisms and the genomic context appear to play much more important roles, affecting the frequency of utilization and the order of activation of multiple initiation sites. Finally, these results confirm that initiation sites are extremely redundant elements of the EBV genome. We propose that these conclusions also apply to mammalian chromosomes.

  19. The HAT-P-7 and HAT-P-11 Star-Planet Systems

    Science.gov (United States)

    Medina, Jennifer; Manrique, Maria; van Hamme, Walter

    2016-03-01

    Radial velocities and KEPLER light curves of the transiting exoplanets HAT-P-7b and HAT-P-11b are analyzed using a general binary star model. The HAT-P-7 system has been a subject of interest due to the irregular orbit of the HAT-P-7b exoplanet with respect to its parent star. HAT-P-7b's orbit has shed light on the diversity of systems that exist in our observable Universe. HAT-P-11 hosts a K type variable star which we were able to analyze with a fourier fit on the light curve data. It is also a host to exoplanet HAT-P-11b which has a slightly inclined orbit. Using our own software, we have calculated the parameters for star-planet pair of HAT-P-7 and HAT-P-11. This presents another way for astronomers to study these complex systems.

  20. New year, old hat

    Science.gov (United States)

    American Geological Institute; American Institute of Physics; American Associationthe Advancement of Science; Carlowicz, Michael

    It is only August, but October 1 looms large on the calendar. That is the day when the 1997 fiscal year (FY ‧97) begins for the U.S. federal government, and like last year, that day might come without a budget.In a flurry of summer activity, the U.S. Congress has passed several landmark bills on welfare reform, expansion of health coverage for workers between jobs, a raise in the minimum wage, and a Safe Drinking Water Act. The House of Representatives even passed all 13 of its annual appropriations bills before Congress took its August recess.

  1. HATS-19b, HATS-20b, HATS-21b: Three Transiting Hot-Saturns Discovered by the HATSouth Survey

    CERN Document Server

    Bhatti, W; Hartman, J D; Zhou, G; Penev, K; Bayliss, D; Jordán, A; Brahm, R; Espinoza, N; Rabus, M; Mancini, L; de Val-Borro, M; Bento, J; Ciceri, S; Csubry, Z; Henning, T; Schmidt, B; Arriagada, P; Butler, R P; Crane, J; Shectman, S; Thompson, I; Tan, T G; Suc, V; Lázár, J; Papp, I; Sári, P

    2016-01-01

    We report the discovery by the HATSouth exoplanet survey of three hot-Saturn transiting exoplanets: HATS-19b, HATS-20b, and HATS-21b. The planet host HATS-19 is a slightly evolved V = 13.0 G0 star with [Fe/H] = 0.240, a mass of 1.303 Msun, and a radius of 1.75 Rsun. HATS-19b is in an eccentric orbit (e = 0.30) around this star with an orbital period of 4.5697 days and has a mass of 0.427 Mjup and a highly inflated radius of 1.66 Rjup. The planet HATS-20b has a Saturn-like mass and radius of 0.273 Mjup and 0.776 Rjup respectively. It orbits the V = 13.8 G9V star HATS-20 (Ms = 0.910 Msun; Rs = 0.892 Rsun) with a period of 3.7993 days. Finally, HATS-21 is a V = 12.2 G4V star with [Fe/H] = 0.300, a mass of 1.080 Msun, and a radius of 1.021 Rsun. Its accompanying planet HATS-21b has a 3.5544-day orbital period, a mass of 0.332 Mjup, and a moderately inflated radius of 1.123 Rjup. With the addition of these three very different planets to the growing sample of hot-Saturns, we re-examine the relations between the ob...

  2. Physiological levels of HBB transgene expression from S/MAR element-based replicating episomal vectors.

    Science.gov (United States)

    Sgourou, Argyro; Routledge, Samantha; Spathas, Dionysios; Athanassiadou, Aglaia; Antoniou, Michael N

    2009-08-20

    Replicating episomal vectors (REV) are in principle able to provide long-term transgene expression in the absence of integration into the target cell genome. The scaffold/matrix attachment region (S/MAR) located 5' of the human beta-interferon gene (IFNB1) has been shown to confer a stable episomal replication and retention function within plasmid vectors when stably transfected and selected in mammalian cells. The minimal requirement for the IFNB1 S/MAR to function in DNA replication and episomal retention is transcription through this element. We used the erythroid beta-globin locus control region-beta-globin gene (betaLCR-HBB) microlocus cassette as a model to assess tissue-specific expression from within an IFNB1 S/MAR-based plasmid REV. The betaLCR-HBB plus S/MAR combination constructs provided either high or low levels of transcription through the S/MAR element. Our results show that the betaLCR-HBB microlocus is able to reproducibly and stably express at full physiological levels on an episome copy number basis. In addition, our data show that even low levels of transcription from betaLCR-HBB through the S/MAR element are sufficient to allow efficient episomal replication and retention. These data provide the principles upon which generic and flexible expression cassette-S/MAR-based REVs can be designed for a wide range of applications.

  3. Rapid recombination among transfected plasmids, chimeric episome formation and trans gene expression in Plasmodium falciparum.

    Science.gov (United States)

    Kadekoppala, M; Cheresh, P; Catron, D; Ji, D D; Deitsch, K; Wellems, T E; Seifert, H S; Haldar, K

    2001-02-01

    Although recombination is known to be important to generating diversity in the human malaria parasite P. falciparum, the low efficiencies of transfection and the fact that integration of transfected DNA into chromosomes is observed only after long periods (typically 12 weeks or more) have made it difficult to genetically manipulate the blood stages of this major human pathogen. Here we show that co-transfection of a P. falciparum line with two plasmids, one expressing a green fluorescent protein (gfp) reporter and the other expressing a drug resistance marker (Tgdhfr-ts M23), allowed selection of a population in which about approximately 30% of the parasites produce GFP. In these GFP-producing parasites, the transfected plasmids had recombined into chimeric episomes as large as 20 kb and could be maintained under drug pressure for at least 16 weeks. Our data suggest that chimera formation occurs early (detected by 7--14 days) and that it involves homologous recombination favored by presence of the same P. falciparum 5'hrp3 UTR promoting transcription from each plasmid. This indicates the presence of high levels of homologous recombination activity in blood stage parasites that can be used to drive rapid recombination of newly introduced DNA, study mechanisms of recombination, and introduce genes for trans expression in P. falciparum.

  4. Roscovitine inhibits EBNA1 serine 393 phosphorylation, nuclear localization, transcription, and episome maintenance.

    Science.gov (United States)

    Kang, Myung-Soo; Lee, Eun Kyung; Soni, Vishal; Lewis, Timothy A; Koehler, Angela N; Srinivasan, Viswanathan; Kieff, Elliott

    2011-03-01

    Latent Epstein-Barr virus (EBV) infection causes human lymphomas and carcinomas. EBV usually persists as an episome in malignant cells. EBV episome persistence, replication, and gene expression are dependent on EBNA1 binding to multiple cognate sites in oriP. To search for inhibitors of EBNA1- and oriP-dependent episome maintenance or transcription, a library of 40,550 small molecules was screened for compounds that inhibit EBNA1- and oriP-dependent transcription and do not inhibit EBNA1- and oriP-independent transcription. This screening identified roscovitine, a selective inhibitor of cyclin-dependent kinase 1 (CDK1), CDK2, CDK5, and CDK7. Based on motif predictions of EBNA1 serine 393 as a CDK phosphorylation site and (486)RALL(489) and (580)KDLVM(584) as potential cyclin binding domains, we hypothesized that cyclin binding to EBNA1 may enable CDK1, -2, -5, or -7 to phosphorylate serine 393. We found that Escherichia coli-expressed EBNA1 amino acids 387 to 641 were phosphorylated in vitro by CDK1-, -2-, -5-, and -7/cyclin complexes and serine 393 phosphorylation was roscovitine inhibited. Further, S393A mutation abrogated phosphorylation. S393A mutant EBNA1 was deficient in supporting EBNA1- and oriP-dependent transcription and episome persistence, and roscovitine had little further effect on the diminished S393A mutant EBNA1-mediated transcription or episome persistence. Immunoprecipitated FLAG-EBNA1 was phosphorylated in vitro, and roscovitine inhibited this phosphorylation. Moreover, roscovitine decreased nuclear EBNA1 and often increased cytoplasmic EBNA1, whereas S393A mutant EBNA1 was localized equally in the nucleus and cytoplasm and was unaffected by roscovitine treatment. These data indicate that roscovitine effects are serine 393 specific and that serine 393 is important in EBNA1- and oriPCp-dependent transcription and episome persistence.

  5. Roscovitine Inhibits EBNA1 Serine 393 Phosphorylation, Nuclear Localization, Transcription, and Episome Maintenance▿ †

    Science.gov (United States)

    Kang, Myung-Soo; Lee, Eun Kyung; Soni, Vishal; Lewis, Timothy A.; Koehler, Angela N.; Srinivasan, Viswanathan; Kieff, Elliott

    2011-01-01

    Latent Epstein-Barr virus (EBV) infection causes human lymphomas and carcinomas. EBV usually persists as an episome in malignant cells. EBV episome persistence, replication, and gene expression are dependent on EBNA1 binding to multiple cognate sites in oriP. To search for inhibitors of EBNA1- and oriP-dependent episome maintenance or transcription, a library of 40,550 small molecules was screened for compounds that inhibit EBNA1- and oriP-dependent transcription and do not inhibit EBNA1- and oriP-independent transcription. This screening identified roscovitine, a selective inhibitor of cyclin-dependent kinase 1 (CDK1), CDK2, CDK5, and CDK7. Based on motif predictions of EBNA1 serine 393 as a CDK phosphorylation site and 486RALL489 and 580KDLVM584 as potential cyclin binding domains, we hypothesized that cyclin binding to EBNA1 may enable CDK1, -2, -5, or -7 to phosphorylate serine 393. We found that Escherichia coli-expressed EBNA1 amino acids 387 to 641 were phosphorylated in vitro by CDK1-, -2-, -5-, and -7/cyclin complexes and serine 393 phosphorylation was roscovitine inhibited. Further, S393A mutation abrogated phosphorylation. S393A mutant EBNA1 was deficient in supporting EBNA1- and oriP-dependent transcription and episome persistence, and roscovitine had little further effect on the diminished S393A mutant EBNA1-mediated transcription or episome persistence. Immunoprecipitated FLAG-EBNA1 was phosphorylated in vitro, and roscovitine inhibited this phosphorylation. Moreover, roscovitine decreased nuclear EBNA1 and often increased cytoplasmic EBNA1, whereas S393A mutant EBNA1 was localized equally in the nucleus and cytoplasm and was unaffected by roscovitine treatment. These data indicate that roscovitine effects are serine 393 specific and that serine 393 is important in EBNA1- and oriPCp-dependent transcription and episome persistence. PMID:21209116

  6. The Starspots of HAT-P-11: Evidence for a Solar-like Dynamo

    Science.gov (United States)

    Morris, Brett M.; Hebb, Leslie; Davenport, James R. A.; Rohn, Graeme; Hawley, Suzanne L.

    2017-09-01

    We measure the starspot radii and latitude distribution on the K4 dwarf HAT-P-11 from Kepler short-cadence photometry. We take advantage of starspot occultations by HAT-P-11’s highly misaligned planet to compare the spot size and latitude distributions to those of sunspots. We find that HAT-P-11’s spots are distributed in latitude much like sunspots near the solar activity maximum, with a mean spot latitude of ≈16° ± 1°. The majority of HAT-P-11’s starspots have physical sizes that closely resemble the sizes of sunspots at solar maximum. We estimate the mean spotted area coverage on HAT-P-11 to be {3}-1+6 % , roughly two orders of magnitude greater than the typical solar spotted area.

  7. Incidence of Hospital Acquired Thrombosis (HAT) in a Tertiary Care Hospital.

    LENUS (Irish Health Repository)

    Khan, MI

    2017-04-01

    Venous thromboembolism (VTE) is a major cause of preventable morbidity and mortality in hospitalized patients. In spite of guidelines, VTE prophylaxis continues to be underutilised, and hospital acquired thrombosis (HAT) continues to be a problem. This study was conducted to estimate the incidence of HAT in a tertiary referral centre and to examine whether VTE risk assessment and thromboprophylaxis (TP) were implemented. Patients 18 years and above, with a radiologically-confirmed acute VTE during the study period of 15 weeks were included. Acute VTE was diagnosed in 100 patients and HAT was diagnosed in 48. There were 12,024 admissions over the study period, therefore the incidence of HAT was 0.4%. TP was prescribed in only 35% of patients, and 65% did not receive any or appropriate TP. Hospitals without active implementation of a formal risk assessment tool and TP policy are likely to continue to have increased incidence of HAT.

  8. ERRATUM: Work smart, wear your hard hat

    CERN Multimedia

    2003-01-01

    An error appeared in the article «Work smart, wear your hard hat» published in Weekly Bulletin 27/2003, page 5. The impact which pierced a hole in the hard hat worn by Gerd Fetchenhauer was the equivalent of a box weighing 5 kg and not 50 kg.

  9. Flat hat glass diffractive optical beam shaper

    Science.gov (United States)

    Reichel, Steffen; Petzold, Uwe; Biertuempfel, Ralf; Vogt, Helge

    2009-02-01

    Many laser applications need a homogeneous - so called flat hat - light distribution in the application area. However, many laser emit Gaussian shaped light. The technology of diffractive optical elements (DOE) can be used to shape the Gaussian beam into a flat hat beam at a compact length. SCHOTT presents a DOE design of a flat hat DOE beam shaper made out of optical glass. Here the material glass has the significant advantage of high laser durability, low scattering losses, high resistance to temperature, moisture, and chemicals compared to polymer DOEs. Simulations and measurements on different DOEs for different wavelength, laser beam width, and laser divergence are presented. Surprisingly the flat hat DOE beam shaper depends only weakly on wavelength and beam width but strongly on laser divergence. Based on the good agreement between simulation and measurement an improved flat hat DOE beam shaper is also presented.

  10. Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors

    Directory of Open Access Journals (Sweden)

    Raymond C.B. Wong

    2017-07-01

    Full Text Available We report the generation of the hiPSC line CERAi001-A-6 from primary human dermal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA for p53.

  11. Warm Spitzer Photometry of XO-4b, HAT-P-6b and HAT-P-8b

    CERN Document Server

    Todorov, Kamen O; Knutson, Heather A; Burrows, Adam; Sada, Pedro V; Cowan, Nicolas B; Agol, Eric; Desert, Jean-Michel; Fortney, Jonathan J; Charbonneau, David; Laughlin, Gregory; Langton, Jonathan; Showman, Adam P; Lewis, Nikole K

    2011-01-01

    We have analyzed Warm Spitzer/IRAC observations of the secondary eclipses of three planets, XO-4b, HAT-P-6b and HAT-P-8b. We measure secondary eclipse amplitudes at 3.6{\\mu}m and 4.5{\\mu}m for each target. XO-4b exhibits a stronger eclipse depth at 4.5{\\mu}m than at 3.6{\\mu}m, which is consistent with the presence of a temperature inversion. HAT-P-8b shows a stronger eclipse amplitude at 3.6{\\mu}m, and is best-described by models without a temperature inversion. The eclipse depths of HAT-P-6b can be fitted with models with a small or no temperature inversion. We consider our results in the context of a postulated relationship between stellar activity and temperature inversions and a relationship between irradiation level and planet dayside temperature, as discussed by Knutson et al. (2010) and Cowan & Agol (2011), respectively. Our results are consistent with these hypotheses, but do not significantly strengthen them. To measure accurate secondary eclipse central phases, we require accurate ephemerides. W...

  12. HATS-22b, HATS-23b and HATS-24b: Three new transiting Super-Jupiters from the HATSouth Project

    CERN Document Server

    Bento, Joao; Hartman, Joel; Bakos, Gaspar; Ciceri, Simona; Brahm, Rafael; Bayliss, Daniel; Espinoza, Nestor; Zhou, George; Rabus, Markus; Bhatti, Waqas; Penev, Kaloyan; Csubry, Zoltan; Jordan, Andres; Mancini, Luigi; Henning, Thomas; de Val-Borro, Miguel; Tinney, Chris; Wright, Duncan; Suc, Vincent; Noyes, Robert; Lazar, Jozsef; Papp, Istvan; Sari, Pal

    2016-01-01

    We report the discovery of three moderately high-mass transiting hot Jupiters from the HATSouth survey: HATS-22b, HATS-23b and HATS-24b. These planets add to the numbers of known planets in the ~2MJ regime. HATS-22b is a 2.74+/-0.11 MJ mass and 0.953+0.048/-0.029 RJ radius planet orbiting a V = 13.455 +/- 0.040 sub-silar mass (M_star = 0.759+/-0.019 M_sun; R_star = 0.759+/-0.019 R_sun) K-dwarf host star on an eccentric (e = 0.079 +/- 0.026) orbit. This planet's high planet-to-stellar mass ratio is further evidence that migration mechanisms for hot Jupiters may rely on exciting orbital eccentricities that bring planets closer to their parent stars followed by tidal circularisation. HATS-23b is a 1.478 +/- 0.080 MJ mass and 1.69 +/- 0.24 RJ radius planet on a grazing orbit around a V = 13.901 +/- 0.010 G-dwarf with properties very similar to those of the Sun (M_star = 1.115 +/- 0.054 M_sun; R_star = 1.145 +/- 0.070 R_sun). HATS-24b orbits a moderately bright V = 12.830 +/- 0.010 F-dwarf star (M_star = 1.218 +/-...

  13. "Work smart, wear your hard hat"

    CERN Multimedia

    2003-01-01

    Falling objects and collisions are frequent occurrences in work sites and hazardous areas. Hard hats can help prevent many types of accident and can even save lives. Just imagine an 800 g spanner falling from a 13 m high scaffold onto the head of someone standing below - a nightmare scenario! The impact to the head is equivalent to that of a 5 kg weight falling from 2 metres. That is just what happened to Gerd Fetchenhauer when he was working on the UA1 experiment. Fortunately, he was wearing a hard hat at the time. "That hat saved my life," he explains. "It punched a hole right through the hat and I was a bit dazed for a couple of hours but otherwise I was OK." Since that day, Gerd Fetchenhauer, now working on CMS, is never seen on a work site without his hard hat on. Work sites have proliferated at CERN with the construction of the LHC and its detectors, and the wearing of hard hats is compulsory (not to mention life-saving). In the underground caverns and experiment halls, where gantry cranes and other h...

  14. White or Black Hat? An Economic Analysis of Computer Hacking

    OpenAIRE

    Caitlin Brown

    2015-01-01

    Cyber attacks have increased sharply in recent years. This paper investigates the decision a profit-motivated hacker makes between working as a malicious hacker, called a black hat, and in cybersecurity as a white hat hacker. A key component of the model is the contest between white and black hats for some part of firm output that is vulnerable to attack. White and black hat earnings are increasing, nonlinear functions of the proportion of black hats. Multiple equilibria exist. Increasing the...

  15. Final report. Human artificial episomal chromosome (HAEC) for building large genomic libraries

    Energy Technology Data Exchange (ETDEWEB)

    Jean-Michael H. Vos

    1999-12-09

    Collections of human DNA fragments are maintained for research purposes as clones in bacterial host cells. However for unknown reasons, some regions of the human genome appear to be unclonable or unstable in bacteria. Their team has developed a system using episomes (extrachromosomal, autonomously replication DNA) that maintains large DNA fragments in human cells. This human artificial episomal chromosomal (HAEC) system may prove useful for coverage of these especially difficult regions. In the broader biomedical community, the HAEC system also shows promise for use in functional genomics and gene therapy. Recent improvements to the HAEC system and its application to mapping, sequencing, and functionally studying human and mouse DNA are summarized. Mapping and sequencing the human genome and model organisms are only the first steps in determining the function of various genetic units critical for gene regulation, DNA replication, chromatin packaging, chromosomal stability, and chromatid segregation. Such studies will require the ability to transfer and manipulate entire functional units into mammalian cells.

  16. Accurate episomal HIV 2-LTR circles quantification using optimized DNA isolation and droplet digital PCR

    OpenAIRE

    Eva Malatinkova; Maja Kiselinova; Pawel Bonczkowski; Wim Trypsteen; Peter Messiaen; Jolien Vermeire; Bruno Verhasselt; Karen Vervisch; Linos Vandekerckhove; Ward De Spiegelaere

    2014-01-01

    Introduction: In HIV-infected patients on combination antiretroviral therapy (cART), the detection of episomal HIV 2-LTR circles is a potential marker for ongoing viral replication. Quantification of 2-LTR circles is based on quantitative PCR or more recently on digital PCR assessment, but is hampered due to its low abundance. Sample pre-PCR processing is a critical step for 2-LTR circles quantification, which has not yet been sufficiently evaluated in patient derived samples. Materials and M...

  17. Episomal maintenance of S/MAR-containing non-viral vectors for RPE-based diseases.

    Science.gov (United States)

    Koirala, Adarsha; Conley, Shannon M; Naash, Muna I

    2014-01-01

    The efficacy of non-viral genetic therapies has historically been limited by transient gene expression and vector loss. Scaffold matrix attachment regions (S/MARs) have been shown to augment transcription, promote episomal maintenance, and provide insulator-like function to DNA in in vitro and in vivo systems. Here we explore the ability of S/MAR elements to mediate these effects in retinal pigment epithelial (RPE) cells with the eventual goal of improving the persistence of expression of our non-viral gene delivery tools. We engineered an RPE-specific reporter vector with or without an S/MAR immediately downstream of the eGFP expression cassette. We show that the S/MAR vector is maintained as an episome for up to 1 year. Experiments in which rhodamine-labeled DNA was delivered to the subretinal space of mice show better persistence of the S/MAR-containing vector in the RPE than the non-S/MAR vector. These results suggest that inclusion of the S/MAR region promotes episomal maintenance of plasmid DNA in the RPE after subretinal delivery and that inclusion of this DNA element may be beneficial for non-viral ocular gene transfer.

  18. On the hat problem, its variations, and their applications

    Directory of Open Access Journals (Sweden)

    Marcin Piotr Krzywkowski

    2010-03-01

    Full Text Available The topic of our paper is the hat problem in which each of n players is randomly fitted with a blue or red hat. Then everybody can try to guess simultaneously his own hat color by looking at the hat colors of the other players. The team wins if at least one player guesses his hat color correctly, and no one guesses his hat color wrong; otherwise the team loses. The aim is to maximize the probability of a win. There are known many variations of the hat problem. In this paper we give a comprehensive list of variations considered in the literature. We describe the applications of the hat problem and its variations, and their connections to different areas of science. We give the full bibliography of any papers, books, and electronic publications about the hat problem.

  19. HATS-31b Through HATS-35b: Five Transiting Hot Jupiters Discovered by the HATSouth Survey

    CERN Document Server

    de Val-Borro, M; Brahm, R; Hartman, J D; Espinoza, N; Penev, K; Ciceri, S; Jordán, A; Bhatti, W; Csubry, Z; Bayliss, D; Bento, J; Zhou, G; Rabus, M; Mancini, L; Henning, T; Schmidt, B; Tan, T G; Tinney, C G; Wright, D J; Kedziora-Chudczer, L; Bailey, J; Suc, V; Lázár, J; Papp, I; Sári, P

    2016-01-01

    We report the discovery of five new transiting hot Jupiter planets discovered by the HATSouth survey: HATS-31b through HATS-35b. These planets orbit moderately bright stars with V magnitudes within the range 11.9-14.4mag while the planets span a range of masses 0.88-1.22MJ, and have somewhat inflated radii between 1.23-1.64RJ.These planets can be classified as typical hot Jupiters, with HATS-31b and HATS-35b being moderately inflated gas giant planets with radii of $1.64 \\pm 0.22$ RJ and 1.464+0.069-0.044RJ, respectively, that can be used to constrain inflation mechanisms. All five systems present a higher Bayesian evidence for a fixed circular orbit model than for an eccentric orbit. The orbital periods range from $1.8209993 \\pm 0.0000016$ day for HATS-35b) to $3.377960 \\pm 0.000012$ day for HATS-31b. Additionally, HATS-35b orbits a relatively young F star with an age of $2.13 \\pm 0.51$ Gyr. We discuss the analysis to derive the properties of these systems and compare them in the context of the sample of wel...

  20. The Kaposi Sarcoma Herpesvirus Latency-associated Nuclear Antigen DNA Binding Domain Dorsal Positive Electrostatic Patch Facilitates DNA Replication and Episome Persistence.

    Science.gov (United States)

    Li, Shijun; Tan, Min; Juillard, Franceline; Ponnusamy, Rajesh; Correia, Bruno; Simas, J Pedro; Carrondo, Maria A; McVey, Colin E; Kaye, Kenneth M

    2015-11-20

    Kaposi sarcoma-associated herpesvirus (KSHV) has a causative role in several human malignancies. KSHV latency-associated nuclear antigen (LANA) mediates persistence of viral episomes in latently infected cells. LANA mediates KSHV DNA replication and segregates episomes to progeny nuclei. The structure of the LANA DNA binding domain was recently solved, revealing a positive electrostatic patch opposite the DNA binding surface, which is the site of BET protein binding. Here we investigate the functional role of the positive patch in LANA-mediated episome persistence. As expected, LANA mutants with alanine or glutamate substitutions in the central, peripheral, or lateral portions of the positive patch maintained the ability to bind DNA by EMSA. However, all of the substitution mutants were deficient for LANA DNA replication and episome maintenance. Mutation of the peripheral region generated the largest deficiencies. Despite these deficiencies, all positive patch mutants concentrated to dots along mitotic chromosomes in cells containing episomes, similar to LANA. The central and peripheral mutants, but not the lateral mutants, were reduced for BET protein interaction as assessed by co-immunoprecipitation. However, defects in BET protein binding were independent of episome maintenance function. Overall, the reductions in episome maintenance closely correlated with DNA replication deficiencies, suggesting that the replication defects account for the reduced episome persistence. Therefore, the electrostatic patch exerts a key role in LANA-mediated DNA replication and episome persistence and may act through a host cell partner(s) other than a BET protein or by inducing specific structures or complexes.

  1. Hat problem on graphs with exactly three cycles

    Directory of Open Access Journals (Sweden)

    Tayebe Balegh

    2016-08-01

    Full Text Available This paper is devoted to investigation of the hat problem on graphs with exactly three cycles. In the hat problem, each of $n$ players is randomly fitted with a blue or red hat. Everybody can try to guess simultaneously his own hat color by looking at the hat colors of the other players. The team wins if at least one player guesses his hat color correctly, and no one guesses his hat color wrong; otherwise the team loses. The aim is to maximize the probability of winning. Note that every player can see everybody excluding himself. This problem has been considered on a graph, where the vertices correspond to the players, and a player can see each player to whom he is connected by an edge. We show that the hat number of a graph with exactly three cycles is $\\frac{3}{4}$ if it contains a triangle, and $\\frac{1}{2}$ otherwise.

  2. Wer hat Angst vor Ayn Rand?

    OpenAIRE

    Brühwiler, Claudia Franziska

    2013-01-01

    Das Werk der amerikanischen Schriftstellerin Ayn Rand hat in den USA sowohl die Politik als auch die Populärkultur geprägt. Ihre Ideen finden sich im amerikanischen Kongress wie auch bei den Simpsons. Die neue Übersetzung ihres Hauptwerks macht sie nun auch im deutschsprachigen Raum wieder zugänglich.

  3. James J. Gallagher: Man in the White Hat

    Science.gov (United States)

    Jolly, Jennifer L.; Robinson, Ann

    2014-01-01

    In classic Western movies, the good guy could be frequently identified by his trademark white Stetson hat, whereas the bad guy always wore black. James J. Gallagher wore many hats during his career that spanned over six decades; he too would be known as the "man in the white hat,"--trusted to do the right thing. From 1967 to 1970,…

  4. Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming.

    Science.gov (United States)

    Vdovin, A S; Lupatov, A Yu; Kholodenko, I V; Yarygin, K N

    2015-11-01

    Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 10(5) cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60.

  5. Human Papilloma Viral DNA Replicates as a Stable Episome in Cultured Epidermal Keratinocytes

    Science.gov (United States)

    Laporta, Robert F.; Taichman, Lorne B.

    1982-06-01

    Human papilloma virus (HPV) is poorly understood because systems for its growth in tissue culture have not been developed. We report here that cultured human epidermal keratinocytes could be infected with HPV from plantar warts and that the viral DNA persisted and replicated as a stable episome. There were 50-200 copies of viral DNA per cell and there was no evidence to indicate integration of viral DNA into the cellular genome. There was also no evidence to suggest that viral DNA underwent productive replication. We conclude that cultured human epidermal keratinocytes may be a model for the study of certain aspects of HPV biology.

  6. Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors.

    Science.gov (United States)

    Su, Ruijun Jeanna; Neises, Amanda; Zhang, Xiao-Bing

    2016-01-01

    Peripheral blood is the easy-to-access, minimally invasive, and the most abundant cell source to use for cell reprogramming. The episomal vector is among the best approaches for generating integration-free induced pluripotent stem (iPS) cells due to its simplicity and affordability. Here we describe the detailed protocol for the efficient generation of integration-free iPS cells from peripheral blood mononuclear cells. With this optimized protocol, one can readily generate hundreds of iPS cell colonies from 1 ml of peripheral blood.

  7. Genome-wide cooperation by HAT Gcn5, remodeler SWI/SNF, and chaperone Ydj1 in promoter nucleosome eviction and transcriptional activation.

    Science.gov (United States)

    Qiu, Hongfang; Chereji, Răzvan V; Hu, Cuihua; Cole, Hope A; Rawal, Yashpal; Clark, David J; Hinnebusch, Alan G

    2016-02-01

    Chaperones, nucleosome remodeling complexes, and histone acetyltransferases have been implicated in nucleosome disassembly at promoters of particular yeast genes, but whether these cofactors function ubiquitously, as well as the impact of nucleosome eviction on transcription genome-wide, is poorly understood. We used chromatin immunoprecipitation of histone H3 and RNA polymerase II (Pol II) in mutants lacking single or multiple cofactors to address these issues for about 200 genes belonging to the Gcn4 transcriptome, of which about 70 exhibit marked reductions in H3 promoter occupancy on induction by amino acid starvation. Examining four target genes in a panel of mutants indicated that SWI/SNF, Gcn5, the Hsp70 cochaperone Ydj1, and chromatin-associated factor Yta7 are required downstream from Gcn4 binding, whereas Asf1/Rtt109, Nap1, RSC, and H2AZ are dispensable for robust H3 eviction in otherwise wild-type cells. Using ChIP-seq to interrogate all 70 exemplar genes in single, double, and triple mutants implicated Gcn5, Snf2, and Ydj1 in H3 eviction at most, but not all, Gcn4 target promoters, with Gcn5 generally playing the greatest role and Ydj1 the least. Remarkably, these three cofactors cooperate similarly in H3 eviction at virtually all yeast promoters. Defective H3 eviction in cofactor mutants was coupled with reduced Pol II occupancies for the Gcn4 transcriptome and the most highly expressed uninduced genes, but the relative Pol II levels at most genes were unaffected or even elevated. These findings indicate that nucleosome eviction is crucial for robust transcription of highly expressed genes but that other steps in gene activation are more rate-limiting for most other yeast genes.

  8. HATS-13b and HATS-14b: two transiting hot Jupiters from the HATSouth survey

    CERN Document Server

    Mancini, L; Penev, K; Bakos, G A; Brahm, R; Ciceri, S; Henning, Th; Csubry, Z; Bayliss, D; Zhou, G; Rabus, M; de Val-Borro, M; Espinoza, N; Jordan, A; Suc, V; Bhatti, W; Schmidt, B; Sato, B; Tan, T G; Wright, D J; Tinney, C G; Addison, B C; Noyes, R W; Lazar, J; Papp, I; Sari, P

    2015-01-01

    We report the discovery of HATS-13b and HATS-14b, two hot-Jupiter transiting planets discovered by the HATSouth survey. The host stars are quite similar to each other (HATS-13: V = 13.9 mag, M* = 0.96 Msun, R* = 0.89 Rsun, Teff = 5500 K, [Fe/H] = 0.05; HATS-14: V = 13.8 mag, M* = 0.97 Msun, R* = 0.93 Rsun, Teff = 5350 K, [Fe/H] = 0.33) and both the planets orbit around them with a period of roughly 3 days and a separation of roughly 0.04 au. However, even though they are irradiated in a similar way, the physical characteristics of the two planets are very different. HATS-13b, with a mass of Mp = 0.543 MJ and a radius of Rp = 1.212 RJ, appears as an inflated planet, while HATS-14b, having a mass of Mp = 1.071 MJ and a radius of Rp = 1.039 RJ, is only slightly larger in radius than Jupiter.

  9. Non-viral S/MAR vectors replicate episomally in vivo when provided with a selective advantage.

    Science.gov (United States)

    Wong, S P; Argyros, O; Coutelle, C; Harbottle, R P

    2011-01-01

    The ideal gene therapy vector should enable persistent expression without the limitations of safety and reproducibility. We previously reported that a prototype plasmid vector, containing a scaffold matrix attachment region (S/MAR) domain and the luciferase reporter gene, showed transgene expression for at least 6 months following a single administration to MF1 mice. Following partial hepatectomy of the animals, however, we found no detectable vector replication and subsequent propagation in vivo. To overcome this drawback, we have now developed an in vivo liver selection strategy by which liver cells transfected with an S/MAR plasmid are provided with a survival advantage over non-transfected cells. This allows an enrichment of vectors that are capable of replicating and establishing themselves as extra-chromosomal entities in the liver. Accordingly, a novel S/MAR plasmid encoding the Bcl-2 gene was constructed; Bcl-2 expression confers resistance against apoptosis-mediated challenges by the Fas-activating antibody Jo2. Following hydrodynamic delivery to the livers of mice and frequent Jo2 administrations, we demonstrate that this Bcl-luciferase S/MAR plasmid is indeed capable of providing sustained luciferase reporter gene expression for over 3 months and that this plasmid replicates as an episomal entity in vivo. These results provide proof-of-principle that S/MAR vectors are capable of preventing transgene silencing, are resistant to integration and are able to confer mitotic stability in vivo when provided with a selective advantage.

  10. Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids.

    Science.gov (United States)

    Meraviglia, Viviana; Zanon, Alessandra; Lavdas, Alexandros A; Schwienbacher, Christine; Silipigni, Rosamaria; Di Segni, Marina; Chen, Huei-Sheng Vincent; Pramstaller, Peter P; Hicks, Andrew A; Rossini, Alessandra

    2015-06-05

    Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by forcing the expression of four transcription factors (Oct-4, Sox-2, Klf-4, and c-Myc), typically expressed by human embryonic stem cells (hESCs). Due to their similarity with hESCs, iPSCs have become an important tool for potential patient-specific regenerative medicine, avoiding ethical issues associated with hESCs. In order to obtain cells suitable for clinical application, transgene-free iPSCs need to be generated to avoid transgene reactivation, altered gene expression and misguided differentiation. Moreover, a highly efficient and inexpensive reprogramming method is necessary to derive sufficient iPSCs for therapeutic purposes. Given this need, an efficient non-integrating episomal plasmid approach is the preferable choice for iPSC derivation. Currently the most common cell type used for reprogramming purposes are fibroblasts, the isolation of which requires tissue biopsy, an invasive surgical procedure for the patient. Therefore, human peripheral blood represents the most accessible and least invasive tissue for iPSC generation. In this study, a cost-effective and viral-free protocol using non-integrating episomal plasmids is reported for the generation of iPSCs from human peripheral blood mononuclear cells (PBMNCs) obtained from frozen buffy coats after whole blood centrifugation and without density gradient separation.

  11. Warm Spitzer Photometry of Three Hot Jupiters: HAT-P-3b, HAT-P-4b and HAT-P-12b

    CERN Document Server

    Todorov, Kamen O; Knutson, Heather A; Burrows, Adam; Fortney, Jonathan J; Lewis, Nikole K; Cowan, Nicolas B; Agol, Eric; Desert, Jean-Michel; Sada, Pedro V; Charbonneau, David; Laughlin, Gregory; Langton, Jonathan; Showman, Adam P

    2013-01-01

    We present Warm Spitzer/IRAC secondary eclipse time series photometry of three short-period transiting exoplanets, HAT-P-3b, HAT-P-4b and HAT-P-12b, in both the available 3.6 and 4.5 micron bands. HAT-P-3b and HAT-P-4b are Jupiter-mass, objects orbiting an early K and an early G dwarf stars, respectively. For HAT-P-3b we find eclipse depths of 0.112%+0.015%-0.030% (3.6 micron) and 0.094%+0.016%-0.009% (4.5 micron). The HAT-P-4b values are 0.142%+0.014%-0.016% (3.6 micron) and 0.122%+0.012%-0.014% (4.5micron). The two planets' photometry is consistent with inefficient heat redistribution from their day to night sides (and low albedos), but it is inconclusive about possible temperature inversions in their atmospheres. HAT-P-12b is a Saturn-mass planet and is one of the coolest planets ever observed during secondary eclipse, along with hot Neptune GJ 436b and hot Saturn WASP-29b. We are able to place 3$\\sigma$ upper limits on the secondary eclipse depth of HAT-P-12b in both wavelengths: < 0.042% (3.6 micron) ...

  12. A randomized phase 2 study exploring the role of bevacizumab and a chemotherapy-free approach in HER2-positive metastatic breast cancer: The HAT study (BOOG 2008-2003), a Dutch Breast Cancer Research Group trial.

    Science.gov (United States)

    Drooger, Jan C; van Tinteren, Harm; de Groot, Steffen M; Ten Tije, Albert J; de Graaf, Hiltje; Portielje, Johanneke E A; Jager, Agnes; Honkoop, Aafke; Linn, Sabine C; Kroep, Judith R; Erdkamp, Frans L G; Hamberg, Paul; Imholz, Alex L T; van Rossum-Schornagel, Quirine C; Heijns, Joan B; van Leeuwen-Stok, A Elise; Sleijfer, Stefan

    2016-10-01

    To explore the role of bevacizumab and a chemotherapy-free approach, the authors evaluated the combination of bevacizumab, trastuzumab, and paclitaxel (HAT) and the regimen of trastuzumab and bevacizumab (HA) with the addition of paclitaxel after progression (HA-HAT) as first-line treatment for patients with human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer. In a noncomparative phase 2 trial, patients were randomized between HAT and HA-HAT. The primary endpoint was the progression-free rate at 1 year (1-year PFR). In the HA-HAT group, progression-free survival (PFS) was separately established for HA (PFS1) and HAT (PFS2). Eighty-four patients received HAT (n = 39) or HA-HAT (n = 45). The 1-year PFR was 74.4% (95% confidence interval [CI], 61.8%-89.4%) and 62.2% (95% CI, 49.6%-89.4%) in the HAT and HA-HAT arms, respectively. The median PFS was 19.8 months (95% CI, 14.9-25.6 months) in the HAT arm and 19.6 months (95% CI, 12.0-32.0 months) in the HA-HAT arm. In the HA-HAT arm, the median PFS1 was 10.4 months (95% CI, 6.2-15.0 months), and the median PFS2 was 8.2 months (95% CI, 7.0-12.6 months). The number and severity of adverse events were comparable between the arms. Both HAT and HA-HAT have promising activity in patients with HER2-positive metastatic breast cancer. In particular, starting with only targeted agents and delaying chemotherapy is worth further exploration. Cancer 2016;122:2961-2970. © 2016 American Cancer Society. © 2016 American Cancer Society.

  13. Europe BlackHat Briefings 2005

    OpenAIRE

    Boschini, Matteo

    2005-01-01

    Anche quest'anno si è svolta ad Amsterdam la conferenza BlackHat Briefings – Digital Self-Defense. E' una delle più importanti conferenze sulla sicurezza informatica, nella quale vengono presentate le novità tecniche sia per la difesa che per i penetration test od i vulnerability assessment. L'edizione di quest'anno ha dato particolare rilievo agli assessment di web Applications, senza tuttavia trascurare i più bassi livelli protocollari

  14. Human histone acetyltransferase 1 (Hat1) acetylates lysine 5 of histone H2A in vivo.

    Science.gov (United States)

    Tafrova, Juliana I; Tafrov, Stefan T

    2014-07-01

    The primary structure of Histone Acetyltransferase 1 (Hat1) has been conserved throughout evolution; however, despite its ubiquity, its cellular function is not well characterized. To study its in vivo acetylation pattern and function, we utilized shRNAmir against Hat1 expressed in the well-substantiated HeLa (human cervical cancer) cell line. To reduce the interference by enzymes with similar HAT specificity, we used HeLa cells expressing histone acetyltransferase Tip60 with mutated acetyl-CoA binding site that abrogates its enzyme activity (mutant HeLa-tip60). Two shRNAmir were identified that reduced the expression of the cytoplasmic and nuclear forms of Hat1. Cytosolic protein preparations from these two clones showed decreased levels of acetylation of lysine 5 (K5) and K12 on histone H4, with the concomitant loss of the acetylation of histone H2A at K5. This pattern of decreased acetylation of H2AK5 was well defined in preparations of histone protein and insoluble nuclear-protein (INP) fractions as well. Abrogating the Hat1 expression caused a 74% decrease in colony-forming efficiency of mutant HeLa-tip60 cells, reduced the size of the colonies by 50%, and decreased the amounts of proteins with molecular weights below 35 kDa in the INP fractions.

  15. Smartphone Household Wireless Electroencephalogram Hat

    Directory of Open Access Journals (Sweden)

    Harold Szu

    2013-01-01

    Full Text Available Rudimentary brain machine interface has existed for the gaming industry. Here, we propose a wireless, real-time, and smartphone-based electroencephalogram (EEG system for homecare applications. The system uses high-density dry electrodes and compressive sensing strategies to overcome conflicting requirements between spatial electrode density, temporal resolution, and spatiotemporal throughput rate. Spatial sparseness is addressed by close proximity between active electrodes and desired source locations and using an adaptive selection of N active among 10N passive electrodes to form m-organized random linear combinations of readouts, m≪N≪10N. Temporal sparseness is addressed via parallel frame differences in hardware. During the design phase, we took tethered laboratory EEG dataset and applied fuzzy logic to compute (a spatiotemporal average of larger magnitude EEG data centers in 0.3 second intervals and (b inside brainwave sources by Independent Component Analysis blind deconvolution without knowing the impulse response function. Our main contributions are the fidelity of quality wireless EEG data compared to original tethered data and the speed of compressive image recovery. We have compared our recovery of ill-posed inverse data against results using Block Sparse Code. Future work includes development of strategies to filter unwanted artifact from high-density EEGs (i.e., facial muscle-related events and wireless environmental electromagnetic interferences.

  16. Targeting Specific HATs for Neurodegenerative Disease Treatment: Translating Basic Biology to Therapeutic Possibilities

    Directory of Open Access Journals (Sweden)

    Sheila K. Pirooznia

    2013-03-01

    Full Text Available Dynamic epigenetic regulation of neurons is emerging as a fundamental mechanism by which neurons adapt their transcriptional responses to specific developmental and environmental cues. While defects within the neural epigenome have traditionally been studied in the context of early developmental and heritable cognitive disorders, recent studies point to aberrant histone acetylation status as a key mechanism underlying acquired inappropriate alterations of genome structure and function in post-mitotic neurons during the aging process. Indeed, it is becoming increasingly evident that chromatin acetylation status can be impaired during the lifetime of neurons through mechanisms related to loss of function of histone acetyltransferase (HATs activity. Several HATs have been shown to participate in vital neuronal functions such as regulation of neuronal plasticity and memory formation. As such, dysregulation of such HATs has been implicated in the pathogenesis associated with age-associated neurodegenerative diseases and cognitive decline. In order to counteract the loss of HAT function in neurodegenerative diseases, the current therapeutic strategies involve the use of small molecules called histone deacetylase (HDAC inhibitors that antagonize HDAC activity and thus enhance acetylation levels. Although this strategy has displayed promising therapeutic effects, currently used HDAC inhibitors lack target specificity, raising concerns about their applicability. With rapidly evolving literature on HATs and their respective functions in mediating neuronal survival and higher order brain function such as learning and memory, modulating the function of specific HATs holds new promises as a therapeutic tool in neurodegenerative diseases. In this review, we focus on the recent progress in research regarding epigenetic histone acetylation mechanisms underlying neuronal activity and cognitive function. We discuss the current understanding of specific HDACs and

  17. Accurate episomal HIV 2-LTR circles quantification using optimized DNA isolation and droplet digital PCR

    Directory of Open Access Journals (Sweden)

    Eva Malatinkova

    2014-11-01

    Full Text Available Introduction: In HIV-infected patients on combination antiretroviral therapy (cART, the detection of episomal HIV 2-LTR circles is a potential marker for ongoing viral replication. Quantification of 2-LTR circles is based on quantitative PCR or more recently on digital PCR assessment, but is hampered due to its low abundance. Sample pre-PCR processing is a critical step for 2-LTR circles quantification, which has not yet been sufficiently evaluated in patient derived samples. Materials and Methods: We compared two sample processing procedures to more accurately quantify 2-LTR circles using droplet digital PCR (ddPCR. Episomal HIV 2-LTR circles were either isolated by genomic DNA isolation or by a modified plasmid DNA isolation, to separate the small episomal circular DNA from chromosomal DNA. This was performed in a dilution series of HIV-infected cells and HIV-1 infected patient derived samples (n=59. Samples for the plasmid DNA isolation method were spiked with an internal control plasmid. Results: Genomic DNA isolation enables robust 2-LTR circles quantification. However, in the lower ranges of detection, PCR inhibition caused by high genomic DNA load substantially limits the amount of sample input and this impacts sensitivity and accuracy. Moreover, total genomic DNA isolation resulted in a lower recovery of 2-LTR templates per isolate, further reducing its sensitivity. The modified plasmid DNA isolation with a spiked reference for normalization was more accurate in these low ranges compared to genomic DNA isolation. A linear correlation of both methods was observed in the dilution series (R2=0.974 and in the patient derived samples with 2-LTR numbers above 10 copies per million peripheral blood mononuclear cells (PBMCs, (R2=0.671. Furthermore, Bland–Altman analysis revealed an average agreement between the methods within the 27 samples in which 2-LTR circles were detectable with both methods (bias: 0.3875±1.2657 log10. Conclusions: 2-LTR

  18. Radiologic characterization of the Mexican Hat, Utah, uranium mill tailings remedial action site: Addendum D1

    Energy Technology Data Exchange (ETDEWEB)

    Ludlam, J.R.

    1985-01-01

    This radiologic characterization of the inactive uranium millsite at Mexican Hat, Utah, was conducted by Bendix Field Engineering Corporation for the US Department of Energy (DOE), Grand Junctions Project Office in response to and in accord with a Statement of Work prepared by the DOE Uranium Mill Tailings Remedial Action Project (UMTRAP) Technical Assistance Contractor, Jacobs Engineering Group, Inc. The objective of this project was to determine the horizontal and vertical extent of contamination that exceeds the US Environmental Protection Agency (EPA) standards at the Mexican Hat site. The data presented in this report are required for characterization of the areas adjacent to the Mexican Hat tailings piles and for the subsequent design of cleanup activities. Some on- pile sampling was required to determine the depth of the 15-pCi/g Ra- 226 interface in an area where wind and water erosion has taken place.

  19. HATS-25b through HATS-30b: A Half-dozen New Inflated Transiting Hot Jupiters from the HATSouth Survey

    CERN Document Server

    Espinoza, N; Hartman, J D; Bakos, G Á; Jordán, A; Zhou, G; Mancini, L; Brahm, R; Ciceri, S; Bhatti, W; Csubry, Z; Rabus, M; Penev, K; Bento, J; de Val-Borro, M; Henning, T; Schmidt, B; Suc, V; Wright, D J; Tinney, C G; Tan, T G; Noyes, R

    2016-01-01

    We report six new inflated hot Jupiters (HATS-25b through HATS-30b) discovered using the HATSouth global network of automated telescopes. The planets orbit stars with $V$ magnitudes in the range $\\sim 12-14$ and have masses in the largely populated $0.5M_J-0.7M_J$ region of parameter space but span a wide variety of radii, from $1.17R_J$ to $1.75 R_J$. HATS-25b, HATS-28b, HATS-29b and HATS-30b are typical inflated hot Jupiters ($R_p = 1.17-1.26R_J$) orbiting G-type stars in short period ($P=3.2-4.6$ days) orbits. However, HATS-26b ($R_p = 1.75R_J$, $P = 3.3024$ days) and HATS-27b ($R_p=1.50R_J$, $P=4.6370$ days) stand out as highly inflated planets orbiting slightly evolved F stars just after and in the turn-off points, respectively, which are among the least dense hot Jupiters, with densities of $0.153$ g cm$^{-3}$ and $0.180$ g cm$^{-3}$, respectively. All the presented exoplanets but HATS-27b are good targets for future atmospheric characterization studies, while HATS-27b is a prime target for Rossiter-McL...

  20. HATS-31b through HATS-35b: Five Transiting Hot Jupiters Discovered By the HATSouth Survey

    Science.gov (United States)

    de Val-Borro, M.; Bakos, G. Á.; Brahm, R.; Hartman, J. D.; Espinoza, N.; Penev, K.; Ciceri, S.; Jordán, A.; Bhatti, W.; Csubry, Z.; Bayliss, D.; Bento, J.; Zhou, G.; Rabus, M.; Mancini, L.; Henning, T.; Schmidt, B.; Tan, T. G.; Tinney, C. G.; Wright, D. J.; Kedziora-Chudczer, L.; Bailey, J.; Suc, V.; Durkan, S.; Lázár, J.; Papp, I.; Sári, P.

    2016-12-01

    We report the discovery of five new transiting hot-Jupiter planets discovered by the HATSouth survey, HATS-31b through HATS-35b. These planets orbit moderately bright stars with V magnitudes within the range of 11.9-14.4 mag while the planets span a range of masses of 0.88-1.22 {M}{{J}} and have somewhat inflated radii between 1.23 and 1.64 {R}{{J}}. These planets can be classified as typical hot Jupiters, with HATS-31b and HATS-35b being moderately inflated gas giant planets with radii of 1.64+/- 0.22 {R}{{J}} and {1.464}-0.044+0.069 {R}{{J}}, respectively, that can be used to constrain inflation mechanisms. All five systems present a higher Bayesian evidence for a fixed-circular-orbit model than for an eccentric orbit. The orbital periods range from 1.8209993+/- 0.0000016 day for HATS-35b) to 3.377960+/- 0.000012 day for HATS-31b. Additionally, HATS-35b orbits a relatively young F star with an age of 2.13+/- 0.51 Gyr. We discuss the analysis to derive the properties of these systems and compare them in the context of the sample of well-characterized transiting hot Jupiters known to date. The HATSouth network is operated by a collaboration consisting of Princeton University (PU), the Max Planck Institute für Astronomie (MPIA), the Australian National University (ANU), and the Pontificia Universidad Católica de Chile (PUC). The station at Las Campanas Observatory (LCO) of the Carnegie Institute is operated by PU in conjunction with PUC, the station at the High Energy Spectroscopic Survey (H.E.S.S.) site is operated in conjunction with MPIA, and the station at Siding Spring Observatory (SSO) is operated jointly with ANU. Based in part on data collected at the Subaru Telescope, which is operated by the National Astronomical Observatory of Japan. Based in part on observations made with the MPG 2.2 m and Euler1.2 m Telescopes at the ESO Observatory in La Silla. This paper uses observations obtained with facilities of the Las Cumbres Observatory Global Telescope.

  1. VizieR Online Data Catalog: Spectroscopy and photometry for HAT-P-50--HAT-P-53 (Hartman+, 2015)

    Science.gov (United States)

    Hartman, J. D.; Bhatti, W.; Bakos, G. A.; Bieryla, A.; Kovacs, G.; Latham, D. W.; Csubry, Z.; de Val-Borro, M.; Penev, K.; Buchhave, L. A.; Torres, G.; Howard, A. W.; Marcy, G. W.; Johnson, J. A.; Isaacson, H.; Sato, B.; Boisse, I.; Falco, E.; Everett, M. E.; Szklenar, T.; Fulton, B. J.; Shporer, A.; Kovacs, T.; Hansen, T.; Beky, B.; Noyes, R. W.; Lazar, J.; Papp, I.; Sari, P.

    2016-04-01

    The HATNet network consists of six identical fully automated instruments, with four at Fred Lawrence Whipple Observatory (FLWO) in AZ, and two on the roof of the Submillimeter Array Hangar Building at Mauna Kea Observatory (MKO) in HI. The light-gathering elements of each instrument include an 11cm diameter telephoto lens, a Sloan r filter, and a 4K*4K front-side-illuminated CCD camera. Observations made in 2007 and early 2008 were carried out using a Cousins R filter. The instruments have a field of view of 10.6°*10.6° and a pixel scale of 9"/pixel at the center of an image. Additional time-series photometric measurements were obtained for all four of the systems using Keplercam on the FLWO 1.2m telescope. For HAT-P-50 we also obtained follow-up photometry with the CCD imager on the Byrne Observatory at Sedgwick (BOS) 0.8m telescope, located at Sedgwick Reserve in Santa Ynez Valley, CA, and operated by the Las Cumbres Observatory Global Telescope institute (LCOGT). HAT-P-50 was observed with HAT-10/G316 on 2008 Nov-2009 May, with HAT-5/G364 on 2009 May, with HAT-9/G364 on 2008 Dec-2009 May, with BOS on 2012 Feb 15, on 2012 Feb 21 and on 2012 Apr 08, and with Keplercam on 2012 Feb 18, on 2012 Nov 28, on 2012 Dec 23, on 2013 Jan 14, and on 2013 Jan 17. HAT-P-51 was observed with HAT-6/G164 on 2007 Sep-2008 Feb, with HAT-9/G164 on 2007 Sep-2008 Feb, with HAT-10/G165 on 2010 Sep-2011 Jan, with HAT-5/G165 on 2010 Nov-2011 Feb, with HAT-8/G165 on 2010 Nov-2011 Feb, with HAT-6/G209 on 2010 Nov-2011 Feb, with HAT-9/G209 on 2010 Nov-2011 Feb, with HAT-7/G210 on 2010 Nov-2011 Jan, and with Keplercam on 2011 Oct 21, on 2012 Jan 05, on 2012 Oct 05, on 2012 Oct 26, and on 2012 Nov 12. HAT-P-52 was observed with HAT-5/G212 on 2010 Sep-Nov, with HAT-8/G212 on 2010 Aug-Nov, and with Keplercam on 2010 Dec 23, on 2011 Sep 05, on 2011 Sep 27, on 2011 Nov 21, and on 2012 Jan 07. HAT-P-53 was observed with HAT-6/G164 on 2007 Sep-2008 Feb, with HAT-9/G164 on 2007 Sep-2008 Feb, with

  2. HAT-P-20b--HAT-P-23b: Four Massive Transiting Extrasolar Planets

    CERN Document Server

    Bakos, G Á; Torres, G; Latham, D W; Kovács, Géza; Noyes, R W; Fischer, D A; Johnson, J A; Marcy, G W; Howard, A W; Kipping, D; Esquerdo, G A; Shporer, A; Béky, B; Buchhave, L A; Perumpilly, G; Everett, M; Sasselov, D D; Stefanik, R P; Lázár, J; Papp, I; Sári, P

    2010-01-01

    We report the discovery of four relatively massive (2-7MJ) transiting extrasolar planets. HAT-P-20b orbits a V=11.339 K3 dwarf star with a period P=2.875317+/-0.000004d. The host star has a mass of 0.760+/-0.03 Msun, radius of 0.690+/-0.02 Rsun, Teff=4595+/-80 K, and metallicity [Fe/H]=+0.35+/-0.08. HAT-P-20b has a mass of 7.246+/-0.187 MJ, and radius of 0.867+/-0.033 RJ yielding a mean density of 13.78+/-1.50 gcm^-3 , which is the second highest value among all known exoplanets. HAT-P-21b orbits a V=11.685 G3 dwarf on an eccentric (e=0.2280+/-0.016) orbit, with a period of P=4.1244810+/-000007d. The host star has a mass of 0.95+/-0.04Msun, radius of 1.10+/-0.08Rsun, Teff=5588+/-80K, and [Fe/H]=+0.01+/-0.08. HAT-P-21b has a mass of 4.063+/-0.161MJ, and radius of 1.024+/-0.092RJ. HAT-P-22b orbits the V=9.732 G5 dwarf HD233731, with P=3.2122200+/-0.000009d. The host star has a mass of 0.92+/-0.03Msun, radius of 1.04+/-0.04Rsun, Teff=5302+/-80K, and metallicity of +0.24+/-0.08. The planet has a mass of 2.147+/-0...

  3. Engineering assessment of inactive uranium mill tailings: Mexican Hat Site, Mexican Hat, Utah

    Energy Technology Data Exchange (ETDEWEB)

    None

    1981-09-01

    Ford, Bacon and Davis Utah Inc. has reevaluated the Mexican Hat site in order to revise the March 1977 engineering assessment of the problems resulting from the existence of radioactive uranium mill tailings at Mexican Hat, Utah. This engineering assessment has included the preparation of topographic maps, the performance of core drillings and radiometric measurements sufficient to determine areas and volumes of tailings and radiation exposures of individuals and nearby populations, the investigations of site hydrology and meteorology, and the evaluation and costing of alternative corrective actions. Radon gas released from the 2.2 million tons of tailings at the Mexican Hat site constitutes the most significant environmental impact, although windblown tailings and external gamma radiation also are factors. The four alternative actions presented in this engineering assessment range from millsite decontamination with the addition of 3 m of stabilization cover material to removal of the tailings to remote disposal sites and decontamination of the tailings site. Cost estimates for the four options range from about $15,200,000 for stabilization in place, to about $45,500,000 for disposal at a distance of about 16 mi. Three principal alternatives for the reprocessing of the Mexican Hat tailings were examined: heap leaching; treatment at an existing mill; and reprocessing at a new conventional mill constructed for tailings reprocessing. The cost of the uranium recovered would be about $115/lb of U/sub 3/O/sub 8/ whether by heap leach or conventional plant processes. The spot market price for uranium was $25/lb early in 1981. Reprocessing the Mexican Hat tailings for uranium recovery is not economically attractive under present conditions.

  4. WARM SPITZER PHOTOMETRY OF THREE HOT JUPITERS: HAT-P-3b, HAT-P-4b AND HAT-P-12b

    Energy Technology Data Exchange (ETDEWEB)

    Todorov, Kamen O. [Department of Astronomy and Astrophysics, Pennsylvania State University, University Park, PA 16802 (United States); Deming, Drake [Department of Astronomy, University of Maryland at College Park, College Park, MD 20742 (United States); Knutson, Heather A. [Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125 (United States); Burrows, Adam [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08544 (United States); Fortney, Jonathan J.; Laughlin, Gregory [Department of Astronomy and Astrophysics, University of California at Santa Cruz, Santa Cruz, CA 95064 (United States); Lewis, Nikole K. [Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Cowan, Nicolas B. [Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208 (United States); Agol, Eric [Department of Astronomy, University of Washington, Box 351580, Seattle, WA 98195 (United States); Desert, Jean-Michel [Astronomy Department, California Institute of Technology, Pasadena, CA 91125 (United States); Sada, Pedro V. [Department of Physics and Mathematics, University of Monterrey, Monterrey (Mexico); Charbonneau, David [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Langton, Jonathan [Department of Physics, Principia College, Elsah, IL 62028 (United States); Showman, Adam P. [Lunar and Planetary Laboratory, University of Arizona, Tucson, AZ 85721 (United States)

    2013-06-20

    We present Warm Spitzer/IRAC secondary eclipse time series photometry of three short-period transiting exoplanets, HAT-P-3b, HAT-P-4b and HAT-P-12b, in both the available 3.6 and 4.5 {mu}m bands. HAT-P-3b and HAT-P-4b are Jupiter-mass objects orbiting an early K and an early G dwarf star, respectively. For HAT-P-3b we find eclipse depths of 0.112%+0.015%-0.030% (3.6 micron) and 0.094%+0.016%-0.009% (4.5 {mu}m). The HAT-P-4b values are 0.142%+0.014%-0.016% (3.6 micron) and 0.122%+0.012%-0.014% 4.5 {mu}m). The two planets' photometry is consistent with inefficient heat redistribution from their day to night sides (and low albedos), but it is inconclusive about possible temperature inversions in their atmospheres. HAT-P-12b is a Saturn-mass planet and is one of the coolest planets ever observed during secondary eclipse, along with the hot Neptune GJ 436b and the hot Saturn WASP-29b. We are able to place 3{sigma} upper limits on the secondary eclipse depth of HAT-P-12b in both wavelengths: <0.042% (3.6 {mu}m) and <0.085% (4.5 {mu}m). We discuss these results in the context of the Spitzer secondary eclipse measurements of GJ 436b and WASP-29b. It is possible that we do not detect the eclipses of HAT-P-12b due to high eccentricity, but find that weak planetary emission in these wavelengths is a more likely explanation. We place 3{sigma} upper limits on the |e cos {omega}| quantity (where e is eccentricity and {omega} is the argument of periapsis) for HAT-P-3b (<0.0081) and HAT-P-4b (<0.0042), based on the secondary eclipse timings.

  5. Spin-orbit inclinations of the exoplanetary systems HAT-P-8b, HAT-P-9b, HAT-P-16b, and HAT-P-23b

    Science.gov (United States)

    Moutou, C.; Díaz, R. F.; Udry, S.; Hébrard, G.; Bouchy, F.; Santerne, A.; Ehrenreich, D.; Arnold, L.; Boisse, I.; Bonfils, X.; Delfosse, X.; Eggenberger, A.; Forveille, T.; Lagrange, A.-M.; Lovis, C.; Martinez, P.; Pepe, F.; Perrier, C.; Queloz, D.; Santos, N. C.; Ségransan, D.; Toublanc, D.; Troncin, J. P.; Vanhuysse, M.; Vidal-Madjar, A.

    2011-09-01

    We report the measurement of the spin-orbit angle of the extra-solar planets HAT-P-8 b, HAT-P-9 b, HAT-P-16 b, and HAT-P-23 b, based on spectroscopic observations performed at the Observatoire de Haute-Provence with the SOPHIE spectrograph on the 1.93-m telescope. Radial velocity measurements of the Rossiter-McLaughlin effect show the detection of an apparent prograde, aligned orbit for all systems. The projected spin-orbit angles are found to be λ = -17°+9.2-11.5, -16° ± 8°, -10° ± 16°, and +15° ± 22° for HAT-P-8, HAT-P-9, HAT-P-16, and HAT-P-23, respectively, with corresponding projected rotational velocities of 14.5 ± 0.8, 12.5 ± 1.8, 3.9 ± 0.8, and 7.8 ± 1.6 km s-1. These new results increase to 37 the number of accurately measured spin-orbit angles in transiting extrasolar systems. We conclude by drawing a tentative picture of the global behaviour of orbital alignement, involving the complexity and diversity of possible mechanisms. Based on observations collected with the SOPHIE spectrograph on the 1.93-m telescope at Observatoire de Haute-Provence (CNRS), France, by the SOPHIE Consortium (program 10A.PNP.CONS).

  6. Stable transformation of an episomal protein-tagging shuttle vector in the piscine diplomonad Spironucleus vortens

    Directory of Open Access Journals (Sweden)

    Cronembold Daniela

    2008-04-01

    Full Text Available Abstract Background Diplomonads are common free-living inhabitants of anoxic aquatic environments and are also found as intestinal commensals or parasites of a wide variety of animals. Spironucleus vortens is a putatively commensal diplomonad of angelfish that grows to high cell densities in axenic culture. Genomic sequencing of S. vortens is in progress, yet little information is available regarding molecular and cellular aspects of S. vortens biology beyond descriptive ultrastructural studies. To facilitate the development of S. vortens as an additional diplomonad experimental model, we have constructed and stably transformed an episomal plasmid containing an enhanced green fluorescent protein (GFP tag, an AU1 epitope tag, and a tandem affinity purification (TAP tag. This construct also contains selectable antibiotic resistance markers for both S. vortens and E. coli. Results Stable transformants of S. vortens grew relatively rapidly (within 7 days after electroporation and were maintained under puromycin selection for over 6 months. We expressed the enhanced GFP variant, eGFP, under transcriptional control of the S. vortens histone H3 promoter, and visually confirmed diffuse GFP expression in over 50% of transformants. Next, we generated a histone H3::GFP fusion using the S. vortens conventional histone H3 gene and its native promoter. This construct was also highly expressed in the majority of S. vortens transformants, in which the H3::GFP fusion localized to the chromatin in both nuclei. Finally, we used fluorescence in situ hybridization (FISH of the episomal plasmid to show that the transformed plasmid localized to only one nucleus/cell and was present at roughly 10–20 copies per nucleus. Because S. vortens grows to high densities in laboratory culture, it is a feasible diplomonad from which to purify native protein complexes. Thus, we also included a TAP tag in the plasmid constructs to permit future tagging and subsequent purification

  7. Monitoring of BTX by passive sampling in Hat Yai

    Directory of Open Access Journals (Sweden)

    Proespichaya Kanatharana

    2004-02-01

    Full Text Available Laboratory-built passive samplers were used for monitoring of trace benzene, toluene and xylene (BTX in Hat Yai from 28 July to 12 August, 2003. Sampler bottles contained activated Tenax TA 60/80 meshand a lab-built thermal well were developed and evaluated for the sampling and analysis of BTX. The sampling was carried out for two weeks before the passive samplers were thermally desorbed, trapped ina sampling loop by a laboratory built purge and trap system and analysed by gas chromatography (GC equipped with a flame ionization detector. After optimization and calibration, the developed method showed high selectivity, a good sensitivity with detection limits for BTX of 0.8, 1.1 and 13.0 µg/m3 respectively and an acceptable precision. Ambient BTX measurements were conducted at many monitoring site i.e. hot spots (high exposure, residential areas/work places (common exposure and park (low exposure. The concentration at hot spots range from 3.2 to 5.4 µg/m3 for benzene, 38.0 to 80.3 µg/m3 for toluene and 29.7 to 66.7 µg/m3 for xylene.The low BTX were found at the city periphery (Tesco-Lotus billboard sampling stations, roof level and in Hat Yai Municipal Park but no absolute background concentration could be defined. The monitoring results showed that at higher level from the street surface, the level of BTX tended to decrease and the BTX pollution built up along a street canyon (Sanehanuson Road according to the wind direction. The highest BTX were found at the underground parking, 23.5 725.1 and 267.9 µg/m3 respectively where both WHO guideline for Benzene (16.3 µg/m3 and Toluene (260 µg/m3 were exceeded.

  8. Episomal Nonviral Gene Therapy Vectors Slow Progression of Atherosclerosis in a Model of Familial Hypercholesterolemia

    Directory of Open Access Journals (Sweden)

    Alastair G Kerr

    2016-01-01

    Full Text Available Familial hypercholesterolemia (FH is a life-threatening genetic disorder characterized by elevated levels of plasma low-density lipoprotein cholesterol (LDL-cholesterol. Current attempts at gene therapy for FH have been limited by the use of strong heterologous promoters which lack genomic DNA elements essential for regulated expression. Here, we have combined a minigene vector expressing the human LDLR cDNA from a 10 kb native human LDLR locus genomic DNA promoter element, with an efficient miRNA targeting 3-hydroxy-3-methylgutaryl-coenzyme A reductase (Hmgcr, to further enhance LDLR expression. We show that the combined vector suppresses endogenous Hmgcr transcripts in vivo, leading to an increase in LDLR transgene expression. In a diet-induced Ldlr-/- mouse model of FH, we show that administration of the combined vector reduces atherogenic plasma lipids by ≃32%. Finally, we demonstrate that our episomal nonviral vectors are able to reduce atherosclerosis by ≃40% after 12 weeks in vivo. Taken together, the vector system we describe exploits the normal cellular regulation of the LDLR to provide prolonged expression of LDLR through targeted knockdown of Hmgcr. This novel gene therapy system could act alone, or in synergy with current therapies that modulate intracellular cholesterol, such as statins, greatly enhancing its therapeutic application for FH.

  9. Immunodiagnosis of episomal Banana streak MY virus using polyclonal antibodies to an expressed putative coat protein.

    Science.gov (United States)

    Sharma, Susheel Kumar; Kumar, P Vignesh; Baranwal, Virendra Kumar

    2014-10-01

    A cryptic Badnavirus species complex, known as banana streak viruses (BSV) poses a serious threat to banana production and genetic improvement worldwide. Due to the presence of integrated BSV sequences in the banana genome, routine detection is largely based on serological and nucleo-serological diagnostic methods which require high titre specific polyclonal antiserum. Viral structural proteins like coat protein (CP) are the best target for in vitro expression, to be used as antigen for antiserum production. However, in badnaviruses precise CP sequences are not known. In this study, two putative CP coding regions (p48 and p37) of Banana streak MY virus (BSMYV) were identified in silico by comparison with caulimoviruses, retroviruses and Rice tungro bacilliform virus. The putative CP coding region (p37) was in vitro expressed in pMAL system and affinity purified. The purified fusion protein was used as antigen for raising polyclonal antiserum in rabbit. The specificity of antiserum was confirmed in Western blots, immunosorbent electron microscopy (ISEM) and antigen coated plate-enzyme linked immunosorbent assay (ACP-ELISA). The antiserum (1:2000) was successfully used in ACP-ELISA for specific detection of BSMYV infection in field and tissue culture raised banana plants. The antiserum was also utilized in immuno-capture PCR (IC-PCR) based indexing of episomal BSMYV infection. This is the first report of in silico identification of putative CP region of BSMYV, production of polyclonal antiserum against recombinant p37 and its successful use in immunodetection.

  10. Bacillus subtilis EdmS (formerly PgsE) participates in the maintenance of episomes.

    Science.gov (United States)

    Ashiuchi, Makoto; Yamashiro, Daisuke; Yamamoto, Kento

    2013-09-01

    Extrachromosomal DNA maintenance (EDM) is an important process in molecular breeding and for various applications in the construction of genetically engineered microbes. Here we describe a novel Bacillus subtilis gene involved in EDM function called edmS (formerly pgsE). Functional gene regions were identified using molecular genetics techniques. We found that EdmS is a membrane-associated protein that is crucial for EDM. We also determined that EdmS can change a plasmid vector with an unstable replicon and worse-than-random segregation into one with better-than-random segregation, suggesting that the protein functions in the declustering and/or partitioning of episomes. EdmS has two distinct domains: an N-terminal membrane-anchoring domain and a C-terminal assembly accelerator-like structure, and mutational analysis of edmS revealed that both domains are essential for EDM. Further studies using cells of Bacillus megaterium and itsedmS (formerly capE) gene implied that EdmS has potential as a molecular probe for exploring novel EDM systems.

  11. Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of Entamoeba histolytica.

    Science.gov (United States)

    Tillack, Manuela; Nowak, Nicolas; Lotter, Hannelore; Bracha, Rivka; Mirelman, David; Tannich, Egbert; Bruchhaus, Iris

    2006-09-01

    The protozoan Entamoeba histolytica causes intestinal inflammation and liver abscess. Cysteine proteinases (CPs) have been proposed as important virulence factors for amoebiasis. To test the role of the various CPs for amoeba induced pathology, the three major enzymes of the parasite, namely EhCP1, EhCP2 and EhCP5 accounting for about 90% of total proteinase activity, were overexpressed by stable episomal transfection. Total CP activity of recombinant amoebae increased by three- to six-fold depending on the gene transfected. Interestingly, overexpression of the genes for EhCP1 or EhCP2 increased the activity of the corresponding enzyme only, whereas overexpression of the gene for EhCP5 increased the activity of all three enzymes, which is consistent with enzyme-converting activity of EhCP5. Cytopathic activity, measured by in vitro monolayer disruption, was dramatically increased in ehcp5-transfectants (five-fold) but showed only a modest increase in ehcp1- or ehcp2-transfectants (1.5-2-fold). In addition, overexpression of ehcp5 but not of ehcp1 or ehcp2 significantly increased amoebic liver abscess formation in laboratory animals. Moreover, transfection and overexpression of ehcp5 was able to compensate the reduction of in vivo pathogenicity in parasites, which have been silenced for the gene encoding the pore-forming protein amoebapore A. In summary, these results further support the important role of EhCP5 in E. histolytica pathogenicity.

  12. Spitzer Secondary Eclipses of HAT-P-13b

    Science.gov (United States)

    Hardy, Ryan A.; Harrington, J.; Hardin, M. R.; Madhusudhan, N.; Cubillos, P.; Blecic, J.; Bakos, G.; Hartman, J. D.

    2013-10-01

    HAT-P-13 b is a transiting hot Jupiter with a slightly eccentric orbit (e = 0.010) inhabiting a two-planet system. The two-planet arrangement provides an opportunity to probe the interior structure of HAT-P-13b. Under equilibrium-tide theory and confirmation that the apsides of planets b and c are in alignment, a measurement of the planet's eccentricity can be related to the planet's tidal Love number k2, which describes the central condensation of the planet's mass and its deformation under tidal effects. A measurement of k2 could constrain interior models of HAT-P-13b. HAT-P-13b's orbit is configured favorably for refinement of the eccentricity by secondary eclipse timing observations, which provide direct measurements of ecosω. In 2010, Spitzer observed two secondary eclipses of HAT-P-13b in the 3.6- and 4.5-μm IRAC bandpasses. We present secondary eclipse times and depths; joint models of the HAT-P-13 system that incorporate transit photometry and radial velocity data; and constraints on the atmospheric chemistry of HAT-P-13b that suggest solar-abundance composition without a thermal inversion. Spitzer is operated by the Jet Propulsion Laboratory, California Institute of Technology, under a contract with NASA, which provided support for this work. This work was supported in part by NASA Planetary Atmospheres Grant NNX13AF38G.

  13. Vector Boson + Jets with BlackHat and Sherpa

    OpenAIRE

    Berger, C. F.; Bern, Z.; Dixon, L. J.; F. Febres Cordero; Forde, D.; T. Gleisberg; Ita, H.; Kosower, D. A.; Maître, D.

    2010-01-01

    We review recent NLO QCD results for W,Z + 3-jet production at hadron colliders, computed using BlackHat and SHERPA. We also include some new results for Z + 3-jet production at the LHC at 7 TeV. We report new progress towards the NLO cross section for W + 4-jet production. In particular, we show that the virtual matrix elements produced by BlackHat are numerically stable. We also show that with an improved integrator and tree-level matrix elements from BlackHat, SHERPA produces well-behaved ...

  14. The "Burgundian" hat from Herjolfsnes, Greenland: new discoveries, new dates

    DEFF Research Database (Denmark)

    Smith, Michèle Hayeur; Arneborg, Jette; Smith, Kevin

    2016-01-01

    of the Greenland Norse colony but also its enduring cultural links with continental European fashions, following a comment to this effect published by Nørlund himself. In 1996, the hat was dated to the early fourteenth century by Arneborg, a century earlier than Nørlund’s dating, based on stylistic comparisons...... with European examples. Recent research on North Atlantic textiles led to a re-examination of the hat, with different sections sampled and resubmitted for accelerated mass spectrometry dating. The results suggest that the body of the hat and its crown are of different periods with c. 100 years between them...

  15. Remedial Action Plan for the codisposal and stabilization of the Monument Valley and Mexican Hat uranium mill tailings at Mexican Hat, Utah

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-02-01

    This document is a revision of the original Mexiacan Hat Remedial Action Plan (RAP) and RAP Modification submitted in July 1988 and January 1989, respectively, along with updated design documents. This RAP has been developed to serve a two-fold purpose. It presents the activities proposed by the Department of Energy (DOE) to accomplish long-term stabilization and control of the residual radioactive materials (RRM) from Monument Valley, Arizona, and Mexican Hat, Utah, at the Mexican Hat disposal site. It also serves to document the concurrence of both the Navajo Nation and the Nuclear Regulatory Commission (NRC) in the remedial action. This agreement, upon execution by DOE and the Navajo Nation and concurrence by the NRC, becomes Appendix B of the Cooperative Agreement. This document has been structured to provide a comprehensive understanding of the remedial action proposed for the Monument Valley and Mexican Hat sites. It includes specific design and construction requirements for the remedial action. Pertinent information and data are included with reference given to the supporting documents. Section 2.0 presents the EPA standards, including a discussion of their objectives. Section 3. 0 summarizes the present site characteristics and provides a definition of site-specific problems. Section 4.0 is the site design for the proposed action. Section 5.0 presents the water resources protection strategy. Section 6.0 summarizes the plan for ensuring health and safety protection for the surrounding community and the on- site workers. Section 7.0 lists the responsibilities of the project participants. Section 8.0 describes the features of the long-term surveillance and maintenance plan.

  16. Physical and Functional HAT/HDAC Interplay Regulates Protein Acetylation Balance

    Directory of Open Access Journals (Sweden)

    Alessia Peserico

    2011-01-01

    Full Text Available The balance between protein acetylation and deacetylation controls several physiological and pathological cellular processes, and the enzymes involved in the maintenance of this equilibrium—acetyltransferases (HATs and deacetylases (HDACs—have been widely studied. Presently, the evidences obtained in this field suggest that the dynamic acetylation equilibrium is mostly maintained through the physical and functional interplay between HAT and HDAC activities. This model overcomes the classical vision in which the epigenetic marks of acetylation have only an activating function whereas deacetylation marks have a repressing activity. Given the existence of several players involved in the preservation of this equilibrium, the identification of these complex networks of interacting proteins will likely foster our understanding of how cells regulate intracellular processes and respond to the extracellular environment and will offer the rationale for new therapeutic approaches based on epigenetic drugs in human diseases.

  17. Alte Marchen?--alter Hut. Neue Marchen!--neuer Hut? 3 Moderne Marchen bearbeitet als Horspiele, mit Aufgaben und Ubungen (Old Fairy Tales?--Old Hat. New Fairy Tales!--New Hat? Three Modern Fairy Tales Presented in a Radio Play, with Activities and Drills).

    Science.gov (United States)

    Kage, Michael

    Three German modern "fairy tales" are presented in a radio play format with exercises based on the tales. Detailed suggestions for the exercises and other class activities based on the manual are included. While serving as a comprehension exercise of the preceding text, the exercises and other activities recommended to accompany them depart from…

  18. pEPito: a significantly improved non-viral episomal expression vector for mammalian cells

    Directory of Open Access Journals (Sweden)

    Ogris Manfred

    2010-03-01

    Full Text Available Abstract Background The episomal replication of the prototype vector pEPI-1 depends on a transcription unit starting from the constitutively expressed Cytomegalovirus immediate early promoter (CMV-IEP and directed into a 2000 bp long matrix attachment region sequence (MARS derived from the human β-interferon gene. The original pEPI-1 vector contains two mammalian transcription units and a total of 305 CpG islands, which are located predominantly within the vector elements necessary for bacterial propagation and known to be counterproductive for persistent long-term transgene expression. Results Here, we report the development of a novel vector pEPito, which is derived from the pEPI-1 plasmid replicon but has considerably improved efficacy both in vitro and in vivo. The pEPito vector is significantly reduced in size, contains only one transcription unit and 60% less CpG motives in comparison to pEPI-1. It exhibits major advantages compared to the original pEPI-1 plasmid, including higher transgene expression levels and increased colony-forming efficiencies in vitro, as well as more persistent transgene expression profiles in vivo. The performance of pEPito-based vectors was further improved by replacing the CMV-IEP with the human CMV enhancer/human elongation factor 1 alpha promoter (hCMV/EF1P element that is known to be less affected by epigenetic silencing events. Conclusions The novel vector pEPito can be considered suitable as an improved vector for biotechnological applications in vitro and for non-viral gene delivery in vivo.

  19. Genetic modification of dividing cells using episomally maintained S/MAR DNA vectors.

    Science.gov (United States)

    Wong, Suet-Ping; Harbottle, Richard Paul

    2013-08-13

    The development of episomally maintained DNA vectors to genetically modify dividing cells efficiently and stably, without the risk of integration-mediated genotoxicity, should prove to be a valuable tool in genetic research. In this study, we demonstrate the utility of Scaffold/Matrix Attachment Region (S/MAR) DNA vectors to model the restoration of a functional wild-type copy of the gene folliculin (FLCN) implicated in the renal cancer Birt-Hogg-Dubé (BHD). Inactivation of FLCN has been shown to be involved in the development of sporadic renal neoplasia in BHD. S/MAR-modified BHD tumor cells (named UOK257-FS) show restored stable FLCN expression and have normalized downstream TGFβ signals. We demonstrate that UOK257-FS cells show a reduced growth rate in vitro and suppression of xenograft tumor development in vivo, compared with the original FLCN-null UOK257 cell line. In addition, we demonstrate that mTOR signaling in serum-starved FLCN-restored cells is differentially regulated compared with the FLCN-deficient cell. The novel UOK257-FS cell line will be useful for studying the signaling pathways affected in BHD pathogenesis. Significantly, this study demonstrates the suitability of S/MAR vectors to successfully model the functional expression of a therapeutic gene in a cancer cell line and will aid the identification of novel cancer markers for diagnosis and therapy.Molecular Therapy-Nucleic Acids (2013) 2, e115; doi:10.1038/mtna.2013.40; published online 13 August 2013.

  20. Elliptic Deformed Superalgebra $u_{q,p}(\\hat{{sl}}(m|n))$

    CERN Document Server

    Kojima, Takeo

    2011-01-01

    We introduce the elliptic deformed superalgebra $U_{q,p}(\\hat{sl}(M|N))$ as one parameter deformation of the quantum superalgebra $U_q(\\hat{sl}(M|N))$. We give bosonizations of the elliptic deformed superalgebra $U_{q,p}(\\hat{sl}(1|2))$ and $U_{q,p}(\\hat{sl}(2|1))$ for an arbitrary level $k$.

  1. Environmental Assessment of Remedial Action at the Mexican Hat Uranium Mill Tailings Site, Mexican Hat, Utah

    OpenAIRE

    U.S. Department of Energy

    1987-01-01

    This document assesses the environmental impacts of the proposed remedial action at the Mexican Hat uranium mill tailings site located on the Navajo Reservation in southern Utah. The site covers 235 acres and contains 69 acres of tailings and several of the original mill structures. The Uranium Mill Tailings Radiation Control Act (UMTRCA) of 1978, Public Law 95-604 (PL95-604), authorized the U.S. Department of Energy to clean up the site to reduce the potential health impacts associated wit...

  2. Radiologic characterization of the Mexican Hat, Utah, uranium mill tailings remedial action site: Appendix D, Addenda D1--D7

    Energy Technology Data Exchange (ETDEWEB)

    Ludlam, J.R.

    1985-01-01

    This radiologic characterization of the inactive uranium millsite at Mexican Hat, Utah, was conducted by Bendix Field Engineering Corporation foe the US Department of Energy (DOE), Grand Junction Project Office, in response to and in accord with a Statement of Work prepared by the DOE Uranium Mill tailings Remedial Action Project (UMTRAP) Technical Assistance Contractor, Jacobs Engineering Group, Inc. the objective of this project was to determine the horizontal and vertical extent of contamination that exceeds the US Environmental Protection Agency (EPA) standards at the Mexican Hat site. The data presented in this report are required for characterization of the areas adjacent to the Mexican Hat tailings piles and for the subsequent design of cleanup activities. Some on-pile sampling was required to determine the depth of the 15-pCi/g Ra-226 interface in an area where wind and water erosion has taken place.

  3. Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells.

    Science.gov (United States)

    Megges, Matthias; Oreffo, Richard O C; Adjaye, James

    2016-01-01

    Human bone mesenchymal stromal cells derived from fetal femur 55 days post-conception were reprogrammed to induced pluripotent stem cells using episomal plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC and supplemented with the following pathway inhibitors - TGFβ receptor inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3β inhibitor (CHIR99021) and ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell lines was demonstrated in vitro and by the Pluritest.

  4. HATS-25b through HATS-30b: A Half–dozen New Inflated Transiting Hot Jupiters from the HATSouth Survey

    Science.gov (United States)

    Espinoza, N.; Bayliss, D.; Hartman, J. D.; Bakos, G. Á.; Jordán, A.; Zhou, G.; Mancini, L.; Brahm, R.; Ciceri, S.; Bhatti, W.; Csubry, Z.; Rabus, M.; Penev, K.; Bento, J.; de Val-Borro, M.; Henning, T.; Schmidt, B.; Suc, V.; Wright, D. J.; Tinney, C. G.; Tan, T. G.; Noyes, R.

    2016-10-01

    We report six new inflated hot Jupiters (HATS-25b through HATS-30b) discovered using the HATSouth global network of automated telescopes. The planets orbit stars with V magnitudes in the range of ∼12–14 and have masses in the largely populated 0.5{M}J{--}0.7{M}J region of parameter space but span a wide variety of radii, from 1.17{R}J to 1.75{R}J. HATS-25b, HATS-28b, HATS-29b, and HATS-30b are typical inflated hot Jupiters ({R}p=1.17{--}1.26{R}J) orbiting G–type stars in short period (P = 3.2-4.6 days) orbits. However, HATS-26b ({R}p=1.75{R}J, P=3.3024 days) and HATS-27b ({R}p=1.50{R}J, P=4.6370 days) stand out as highly inflated planets orbiting slightly evolved F stars just after and in the turn–off points, respectively, which are among the least dense hot Jupiters, with densities of 0.153 {{g}} {{cm}}-3 and 0.180 {{g}} {{cm}}-3, respectively. All the presented exoplanets but HATS-27b are good targets for future atmospheric characterization studies, while HATS-27b is a prime target for Rossiter—McLaughlin monitoring in order to determine its spin–orbit alignment given the brightness (V = 12.8) and stellar rotational velocity (v\\sin i≈ 9.3 km s‑1) of the host star. These discoveries significantly increase the number of inflated hot Jupiters known, contributing to our understanding of the mechanism(s) responsible for hot Jupiter inflation. The HATSouth network is operated by a collaboration consisting of Princeton University (PU), the Max Planck Institute für Astronomie (MPIA), the Australian National University (ANU), and the Pontificia Universidad Católica de Chile (PUC). The station at Las Campanas Observatory (LCO) of the Carnegie Institute is operated by PU in conjunction with PUC, the station at the High Energy Spectroscopic Survey (H.E.S.S.) site is operated in conjunction with MPIA, and the station at Siding Spring Observatory (SSO) is operated jointly with ANU. Based in part on observations made with the MPG 2.2 m Telescope at the ESO

  5. A Sequence-Independent Strategy for Amplification and Characterisation of Episomal Badnavirus Sequences Reveals Three Previously Uncharacterised Yam Badnaviruses.

    Science.gov (United States)

    Bömer, Moritz; Turaki, Aliyu A; Silva, Gonçalo; Kumar, P Lava; Seal, Susan E

    2016-07-07

    Yam (Dioscorea spp.) plants are potentially hosts to a diverse range of badnavirus species (genus Badnavirus, family Caulimoviridae), but their detection is complicated by the existence of integrated badnavirus sequences in some yam genomes. To date, only two badnavirus genomes have been characterised, namely, Dioscorea bacilliform AL virus (DBALV) and Dioscorea bacilliform SN virus (DBSNV). A further 10 tentative species in yam have been described based on their partial reverse transcriptase (RT)-ribonuclease H (RNaseH) sequences, generically referred to here as Dioscorea bacilliform viruses (DBVs). Further characterisation of DBV species is necessary to determine which represent episomal viruses and which are only present as integrated badnavirus sequences in some yam genomes. In this study, a sequence-independent multiply-primed rolling circle amplification (RCA) method was evaluated for selective amplification of episomal DBV genomes. This resulted in the identification and characterisation of nine complete genomic sequences (7.4-7.7 kbp) of existing and previously undescribed DBV phylogenetic groups from Dioscorea alata and Dioscorea rotundata accessions. These new yam badnavirus genomes expand our understanding of the diversity and genomic organisation of DBVs, and assist the development of improved diagnostic tools. Our findings also suggest that mixed badnavirus infections occur relatively often in West African yam germplasm.

  6. HAT-P-39b-HAT-P-41b: Three Highly Inflated Transiting Hot Jupiters

    Science.gov (United States)

    Hartman, J. D.; Bakos, G. Á.; Béky, B.; Torres, G.; Latham, D. W.; Csubry, Z.; Penev, K.; Shporer, A.; Fulton, B. J.; Buchhave, L. A.; Johnson, J. A.; Howard, A. W.; Marcy, G. W.; Fischer, D. A.; Kovács, G.; Noyes, R. W.; Esquerdo, G. A.; Everett, M.; Szklenár, T.; Quinn, S. N.; Bieryla, A.; Knox, R. P.; Hinz, P.; Sasselov, D. D.; Fűrész, G.; Stefanik, R. P.; Lázár, J.; Papp, I.; Sári, P.

    2012-11-01

    We report the discovery of three new transiting extrasolar planets orbiting moderately bright (V = 11.1, 11.7, and 12.4) F stars. The planets HAT-P-39b through HAT-P-41b have periods of P = 3.5439 days, 4.4572 days, and 2.6940 days, masses of 0.60 M J, 0.62 M J, and 0.80 M J, and radii of 1.57 R J, 1.73 R J, and 1.68 R J, respectively. They orbit stars with masses of 1.40 M ⊙, 1.51 M ⊙, and 1.51 M ⊙, respectively. The three planets are members of an emerging population of highly inflated Jupiters with 0.4 M J 1.5 R J. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NOAO (A201Hr, A289Hr, and A284Hr), NASA (N049Hr, N018Hr, N167Hr, N029Hr, N108Hr, and N154Hr), and the NOAO Gemini/Keck time-exchange program (G329Hr). Based in part on observations made with the Nordic Optical Telescope, operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway, and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias. Based in part on observations obtained with facilities of the Las Cumbres Observatory Global Telescope. Observations reported here were obtained at the MMT Observatory, a joint facility of the Smithsonian Institution and the University of Arizona.

  7. HATS-7b: A Hot Super Neptune Transiting a Quiet K Dwarf Star

    CERN Document Server

    Bakos, G Á; Bayliss, D; Hartman, J D; Zhou, G; Brahm, R; Mancini, L; deVal-Borro, M; Bhatti, W; Jordán, A; Rabus, M; Espinoza, N; Csubry, Z; Howard, A W; Fulton, B J; Buchhave, L A; Ciceri, S; Henning, T; Schmidt, B; Isaacson, H; Noyes, R W; Marcy, G W; Suc, V; Howe, A R; Burrows, A S; Lázár, J; Papp, I; Sári, P

    2015-01-01

    We report the discovery by the HATSouth network of HATS-7b, a transiting Super-Neptune with a mass of 0.120+/-0.012 M_Jup, a radius of 0.563+0.046-0.034 R_Jup, and an orbital period of 3.1853 days. The host star is a moderately bright (V = 13.340+/-0.010 mag, K_S = 10.976+/-0.026 mag) K dwarf star with a mass of 0.849+/-0.027 M_Sun, a radius of 0.815+0.049-0.035 R_Sun, and a metallicity of [Fe/H]= +0.250+/-0.080. The star is photometrically quiet to within the precision of the HATSouth measurements, has low RV jitter, and shows no evidence for chromospheric activity in its spectrum. HATS-7b is the second smallest radius planet discovered by a wide-field ground-based transit survey, and one of only a handful of Neptune-size planets with mass and radius determined to 10% precision. Theoretical modeling of HATS-7b yields a hydrogen-helium fraction of 18+/-4% (rock-iron core and H2-He envelope), or 9+/-4% (ice core and H2-He envelope), i.e.it has a composition broadly similar to that of Uranus and Neptune, and ve...

  8. HAT-P-20b-HAT-P-23b: Four Massive Transiting Extrasolar Planets

    Science.gov (United States)

    Bakos, G. Á.; Hartman, J.; Torres, G.; Latham, D. W.; Kovács, Géza; Noyes, R. W.; Fischer, D. A.; Johnson, J. A.; Marcy, G. W.; Howard, A. W.; Kipping, D.; Esquerdo, G. A.; Shporer, A.; Béky, B.; Buchhave, L. A.; Perumpilly, G.; Everett, M.; Sasselov, D. D.; Stefanik, R. P.; Lázár, J.; Papp, I.; Sári, P.

    2011-12-01

    We report the discovery of four relatively massive (2-7 M J) transiting extrasolar planets. HAT-P-20b orbits the moderately bright V = 11.339 K3 dwarf star GSC 1910-00239 on a circular orbit, with a period P = 2.875317 ± 0.000004 days, transit epoch Tc = 2455080.92661 ± 0.00021 (BJDUTC), and transit duration 0.0770 ± 0.0008 days. The host star has a mass of 0.76 ± 0.03 M ⊙, radius of 0.69 ± 0.02 R ⊙, effective temperature 4595 ± 80 K, and metallicity [Fe/H] = +0.35 ± 0.08. The planetary companion has a mass of 7.246 ± 0.187 M J and a radius of 0.867 ± 0.033 R J yielding a mean density of 13.78 ± 1.50 g cm-3. HAT-P-21b orbits the V = 11.685 G3 dwarf star GSC 3013-01229 on an eccentric (e = 0.228 ± 0.016) orbit, with a period P = 4.124481 ± 0.000007 days, transit epoch Tc = 2454996.41312 ± 0.00069, and transit duration 0.1530 ± 0.0027 days. The host star has a mass of 0.95 ± 0.04 M ⊙, radius of 1.10 ± 0.08 R ⊙, effective temperature 5588 ± 80 K, and metallicity [Fe/H] = +0.01 ± 0.08. The planetary companion has a mass of 4.063 ± 0.161 M J and a radius of 1.024 ± 0.092 R J yielding a mean density of 4.68+1.59 - 0.99 g cm-3. HAT-P-21b is a borderline object between the pM and pL class planets, and the transits occur near apastron. HAT-P-22b orbits the bright V = 9.732 G5 dwarf star HD 233731 on a circular orbit, with a period P = 3.212220 ± 0.000009 days, transit epoch Tc = 2454930.22001 ± 0.00025, and transit duration 0.1196 ± 0.0014 days. The host star has a mass of 0.92 ± 0.03 M ⊙, radius of 1.04 ± 0.04 R ⊙, effective temperature 5302 ± 80 K, and metallicity [Fe/H] = +0.24 ± 0.08. The planet has a mass of 2.147 ± 0.061 M J and a compact radius of 1.080 ± 0.058 R J yielding a mean density of 2.11+0.40 - 0.29 g cm-3. The host star also harbors an M-dwarf companion at a wide separation. Finally, HAT-P-23b orbits the V = 12.432 G0 dwarf star GSC 1632-01396 on a close to circular orbit, with a period P = 1.212884 ± 0.000002 days

  9. Vector Boson + Jets with BlackHat and SHERPA

    CERN Document Server

    Berger, C F; Dixon, L J; Cordero Febres, F; Forde, D; Gleisberg, T; Ita, H; Kosower, D A; Maitre, D

    2010-01-01

    We review recent NLO QCD results for W,Z + 3-jet production at hadron colliders, computed using BlackHat and SHERPA. We also include some new results for Z + 3-jet production at the LHC at 7 TeV. We report new progress towards the NLO cross section for W + 4-jet production. In particular, we show that the virtual matrix elements produced by BlackHat are numerically stable. We also show that with an improved integrator and tree-level matrix elements from BlackHat, SHERPA produces well-behaved real-emission contributions. As an illustration, we present the real-emission contributions -- including dipole-subtraction terms -- to the p_T distribution of the fourth jet, for a single subprocess with the maximum number of gluons.

  10. Vector Boson Jets with BlackHat and Sherpa

    Energy Technology Data Exchange (ETDEWEB)

    Berger, C.F.; /MIT, LNS; Bern, Z.; /UCLA; Dixon, Lance J.; /SLAC; Cordero, F.Febres; /Simon Bolivar U.; Forde, D.; /CERN /NIKHEF, Amsterdam; Gleisberg, T.; /SLAC; Ita, H.; /UCLA; Kosower, D.A.; /Saclay, SPhT; Maitre, D.; /Durham U.

    2010-08-25

    We review recent NLO QCD results for W, Z + 3-jet production at hadron colliders, computing using BlackHat and SHERPA, and including also some new results for Z + 3-jet production for the LHC at 7 TeV. We report new progress towards the NLO cross section for W + 4-jet production. In particular, we show that the virtual matrix elements produced by BlackHat are numerically stable. We also show that with an improved integrator and tree-level matrix elements from BlackHat, SHERPA produces well-behaved real-emission contributions. As an illustration, we present the real-emission contributions - including dipole-subtraction terms - to the p{sub T} distribution of the fourth jet, for a single subprocess with the maximum number of gluons.

  11. Social factors in occupational health: a history of hard hats.

    Science.gov (United States)

    Rosenberg, Beth; Levenstein, Charles

    2010-01-01

    Personal protective equipment (PPE) is the least desirable way to ensure workplace safety, and it is difficult to use consistently. Hard hats are different; they have cachet and are often worn even when they are not required. We investigated the history of this personal protective equipment to see if there were any lessons that could be applied to other forms of PPE. We learned that what makes hard hats special are social factors that are specific to a certain time and place. The importance of social factors illuminates the requirement that cultural and social norms of workers be included in any kind of worker safety and health training.

  12. HAT AVERAGE MULTIRESOLUTION WITH ERROR CONTROL IN 2-D

    Institute of Scientific and Technical Information of China (English)

    Sergio Amat

    2004-01-01

    Multiresolution representations of data are a powerful tool in data compression. For a proper adaptation to the singularities, it is crucial to develop nonlinear methods which are not based on tensor product. The hat average framework permets develop adapted schemes for all types of singularities. In contrast with the wavelet framework these representations cannot be considered as a change of basis, and the stability theory requires different considerations. In this paper, non separable two-dimensional hat average multiresolution processing algorithms that ensure stability are introduced. Explicit error bounds are presented.

  13. Orbital Orientations of Exoplanets: HAT-P-4b is Prograde and HAT-P-14b is Retrograde

    CERN Document Server

    Winn, Joshua N; Johnson, John Asher; Marcy, Geoffrey W; Isaacson, Howard; Shporer, Avi; Bakos, Gaspar A; Hartman, Joel D; Holman, Matthew J; Albrecht, Simon

    2010-01-01

    We present observations of the Rossiter-McLaughlin effect for two exoplanetary systems, revealing the orientations of their orbits relative to the rotation axes of their parent stars. HAT-P-4b is prograde, with a sky-projected spin-orbit angle of lambda = -4.9 +/- 11.9 degrees. In contrast, HAT-P-14b is retrograde, with lambda = 187.8 +/- 4.4 degrees. These results conform with a previously noted pattern among the stellar hosts of close-in giant planets: hotter stars have a wide range of obliquities and cooler stars have low obliquities. This, in turn, suggests that three-body dynamics and tidal dissipation are responsible for the short-period orbits of many exoplanets. In addition, our data revealed a third body in the HAT-P-4 system, which could be a second planet or a companion star.

  14. Survey of sugar beet (Beta vulgaris L.) hAT transposons and MITE-like hATpin derivatives.

    Science.gov (United States)

    Menzel, Gerhard; Krebs, Carmen; Diez, Mercedes; Holtgräwe, Daniela; Weisshaar, Bernd; Minoche, André E; Dohm, Juliane C; Himmelbauer, Heinz; Schmidt, Thomas

    2012-03-01

    Genome-wide analyses of repetitive DNA suggest a significant impact particularly of transposable elements on genome size and evolution of virtually all eukaryotic organisms. In this study, we analyzed the abundance and diversity of the hAT transposon superfamily of the sugar beet (B. vulgaris) genome, using molecular, bioinformatic and cytogenetic approaches. We identified 81 transposase-coding sequences, three of which are part of structurally intact but nonfunctional hAT transposons (BvhAT), in a B. vulgaris BAC library as well as in whole genome sequencing-derived data sets. Additionally, 116 complete and 497 truncated non-autonomous BvhAT derivatives lacking the transposase gene were in silico-detected. The 116 complete derivatives were subdivided into four BvhATpin groups each characterized by a distinct terminal inverted repeat motif. Both BvhAT and BvhATpin transposons are specific for species of the genus Beta and closely related species, showing a localization on B. vulgaris chromosomes predominantely in euchromatic regions. The lack of any BvhAT transposase function together with the high degree of degeneration observed for the BvhAT and the BvhATpin genomic fraction contrasts with the abundance and activity of autonomous and non-autonomous hAT transposons revealed in other plant species. This indicates a possible genus-specific structural and functional repression of the hAT transposon superfamily during Beta diversification and evolution.

  15. Hats off to Problem-Solving with Couples

    Science.gov (United States)

    Li, Chi-Sing; Lin, Yu-Fen; Nelson, Judy; Eckstein, Daniel

    2008-01-01

    The purpose of this article is to demonstrate how counselors can use de Bono's Six Thinking Hats problem-solving technique in their work with couples. Part 1 of the article focuses on an introduction to the technique, including a theoretical rationale and supporting research. Following a detailed description of the process of using the model as a…

  16. Hard-Hat Detection for Construction Safety Visualization

    Directory of Open Access Journals (Sweden)

    Kishor Shrestha

    2015-01-01

    Full Text Available In 2012, 775 fatalities were recorded, and many more were injured at construction sites in the United States. Of these, 415 fatalities (54% were due to fall, slips, and trips as well as being struck by falling objects. In order to decrease fatalities at construction sites to these types of events, the Occupational Safety and Health Administration (OSHA provides Fall Prevention and OSHA-10 trainings to construction workers. Moreover, safety personnel monitor whether the workers use personal protective equipment (PPE properly. Data shows that construction fatalities have decreased by 2% annually since 1994; however, the owners still are not satisfied with this result. Various studies have shown that fall is the biggest contributor for construction fatalities. One study showed that half of the fall fatalities were because the workers either had not used PPEs or had not used them properly. In addition, studies showed that, with proper use of hard hats, the fatalities due to fall, slips, trips, and being struck by falling objects could be reduced. This study developed and tested a hard-hat detection tool that uses image-processing techniques to identify whether workers are wearing hard hats. The tool dispatches warning messages if the workers do not use hard hats.

  17. Exoplanet Transits Registered at the Universidad de Monterrey Observatory. Part I: HAT-P-12b, HAT-P-13b, HAT-P-16b, HAT-P-23b and WASP-10b

    CERN Document Server

    Sada, Pedro V

    2016-01-01

    Forty transits of the exoplanets HAT-P-12b, HAT-P-13b, HAT-P-16b, HAT-P-23b and WASP-10b were recorded with the 0.36m telescope at the Universidad de Monterrey Observatory. The images were captured with a standard Johnson-Cousins Rc and Ic and Sloan z' filters and processed to obtain individual light curves of the events. These light curves were successfully combined for each system to obtain a resulting one of higher quality, but with a slightly larger time sampling rate. A reduction by a factor of about four in per-point scatter was typically achieved, resulting in combined light curves with a scatter of ~1 mmag. The noise characteristics of the combined light curves were verified by comparing Allan variance plots of the residuals. The combined light curves for each system, along with radial velocity measurements from the literature when available, were modeled using a Monte Carlo method to obtain the essential parameters that characterize the systems. Our results for all these systems confirm the derived t...

  18. The long duration flight of the TopHat experiment

    DEFF Research Database (Denmark)

    Silverberg, R. F.; Aguirre, J.; Bezaire, J.;

    2003-01-01

    The TopHat instrument was designed to operate on the top of a high altitude balloon. From this location, the experiment could efficiently observe using a clean beam with extremely low contamination from the far side lobes of the instrument beam. The experiment was designed to scan a large portion...

  19. Broad-band spectrophotometry of HAT-P-32 b

    DEFF Research Database (Denmark)

    Mallonn, M.; Bernt, I.; Herrero, E.

    2016-01-01

    Multicolour broad-band transit observations offer the opportunity to characterize the atmosphere of an extrasolar planet with small- to medium-sized telescopes. One of the most favourable targets is the hot Jupiter HAT-P-32 b. We combined 21 new transit observations of this planet with 36 previou...

  20. Dr. Edward de Bono's Six Thinking Hats and Numeracy

    Science.gov (United States)

    Paterson, Anne

    2006-01-01

    In education, the term "metacognition" describes thinking about thinking. Within mathematics, the term "metacomputation" describes thinking about computational methods and tools. This article shows how Dr. Edward de Bono's Six Thinking Hats can be used to demonstrate metacognition and metacomputation in the primary classroom. The article suggests…

  1. HATS: A Design Procedure for Routine Business Documents.

    Science.gov (United States)

    Baker, William H.

    2001-01-01

    Describes an approach to teaching students a basic design process for routine business documents like memos, letters, and reports. Outlines the design principles of HATS (Headings, Access, Typography, and Spacing), how they apply in before-and-after fashion to various documents, and discusses an assignment in which students redesign an existing…

  2. Human glioblastoma cells persistently infected with simian virus 40 carry nondefective episomal viral DNA and acquire the transformed phenotype and numerous chromosomal abnormalities.

    Science.gov (United States)

    Norkin, L C; Steinberg, V I; Kosz-Vnenchak, M

    1985-02-01

    A stable, persistent infection of A172 human glioblastoma cells with simian virus 40 (SV40) was readily established after infection at an input of 450 PFU per cell. Only 11% of the cells were initially susceptible to SV40, as shown by indirect immunofluorescent staining for the SV40 T antigen at 48 h. However, all cells produced T antigen by week 11. In contrast, viral capsid proteins were made in only about 1% of the cells in the established carrier system. Weekly viral yields ranged between 10(4) and 10(6) PFU/ml. Most of the capsid protein-producing cells contained enormous aberrant (lobulated or multiple) nuclei. Persistent viral DNA appeared in an episomal or "free" state exclusively in Southern blots and was indistinguishable from standard SV40 DNA by restriction analysis. Viral autointerference activity was not detected, and yield reduction assays did not indicate defective interfering particle activity, further implying that variant viruses were not a factor in this carrier system. Interferon was also not a factor in the system, as shown by direct challenge with vesicular stomatitis virus. Persistent infection resulted in cellular growth changes (enhanced saturation density and plating efficiency) characteristic of SV40 transformation. Persistent infection also led to an increased frequency of cytogenetic effects. These included sister chromatid exchanges, a variety of chromosomal abnormalities (ring chromosomes, acentric fragments, breaks, and gaps), and an increase in the chromosome number. Nevertheless, the persistently infected cells continued to display a bipolar glial cell-like morphology with extensive process extension and intercellular contacts.

  3. Ectopic expression of AID in a non-B cell line triggers A:T and G:C point mutations in non-replicating episomal vectors.

    Directory of Open Access Journals (Sweden)

    Tihana Jovanic

    Full Text Available Somatic hypermutation (SHM of immunoglobulin genes is currently viewed as a two step process initiated by the deamination of deoxycytidine (C to deoxyuridine (U, catalysed by the activation induced deaminase (AID. Phase 1 mutations arise from DNA replication across the uracil residue or the abasic site, generated by the uracil-DNA glycosylase, yielding transitions or transversions at G:C pairs. Phase 2 mutations result from the recognition of the U:G mismatch by the Msh2/Msh6 complex (MutS Homologue, followed by the excision of the mismatched nucleotide and the repair, by the low fidelity DNA polymerase eta, of the gap generated by the exonuclease I. These mutations are mainly focused at A:T pairs. Whereas in activated B cells both G:C and A:T pairs are equally targeted, ectopic expression of AID was shown to trigger only G:C mutations on a stably integrated reporter gene. Here we show that when using non-replicative episomal vectors containing a GFP gene, inactivated by the introduction of stop codons at various positions, a high level of EGFP positive cells was obtained after transient expression in Jurkat cells constitutively expressing AID. We show that mutations at G:C and A:T pairs are produced. EGFP positive cells are obtained in the absence of vector replication demonstrating that the mutations are dependent only on the mismatch repair (MMR pathway. This implies that the generation of phase 1 mutations is not a prerequisite for the expression of phase 2 mutations.

  4. HAT-P-50b, HAT-P-51b, HAT-P-52b, and HAT-P-53b: Three Transiting Hot Jupiters and a Transiting Hot Saturn From the HATNet Survey

    CERN Document Server

    Hartman, Joel D; Bakos, Gáspár Á; Bieryla, Allyson; Kovács, Géza; Latham, David W; Csubry, Zoltan; de Val-Borro, Miguel; Penev, Kaloyan; Buchhave, Lars A; Torres, Guillermo; Howard, Andrew W; Marcy, Geoff W; Johnson, John A; Isaacson, Howard; Sato, Bun'ei; Boisse, Isabelle; Falco, Emilio; Everett, Mark E; Szklenar, Tamas; Fulton, Benjamin J; Shporer, Avi; Kovács, Tamas; Hansen, Terese; Béky, Bence; Noyes, Robert W; Lázár, József; Papp, Istvan; Sári, Pál

    2015-01-01

    We report the discovery and characterization of four transiting exoplanets by the HATNet survey. The planet HAT-P-50b has a mass of 1.35 M_J and a radius of 1.29 R_J, and orbits a bright (V = 11.8 mag) M = 1.27 M_sun, R = 1.70 R_sun star every P = 3.1220 days. The planet HAT-P-51b has a mass of 0.31 M_J and a radius of 1.29 R_J, and orbits a V = 13.4 mag, M = 0.98 M_sun, R = 1.04 R_sun star with a period of P = 4.2180 days. The planet HAT-P-52b has a mass of 0.82 M_J and a radius of 1.01 R_J, and orbits a V = 14.1 mag, M = 0.89 M_sun, R = 0.89 R_sun star with a period of P = 2.7536 days. The planet HAT-P-53b has a mass of 1.48 M_J and a radius of 1.32 R_J, and orbits a V = 13.7 mag, M = 1.09 M_sun, R = 1.21 R_sun star with a period of P = 1.9616 days. All four planets are consistent with having circular orbits and have masses and radii measured to better than 10% precision. The low stellar jitter and favorable R_P/R_star ratio for HAT-P-51 make it a promising target for measuring the Rossiter-McLaughlin effec...

  5. HATS-9b AND HATS-10b: TWO COMPACT HOT JUPITERS IN FIELD 7 OF THE K2 MISSION

    CERN Document Server

    Brahm, R; Hartman, J D; Bakos, G Á; Bayliss, D; Penev, K; Zhou, G; Ciceri, S; Rabus, M; Espinoza, N; Mancini, L; de Val-Borro, M; Bhatti, W; Sato, B; Tan, T G; Csubry, Z; Buchhave, L; Henning, T; Schmidt, B; Suc, V; Noyes, R W; Papp, I; Lázár, J; Sári, P

    2015-01-01

    We report the discovery of two transiting extrasolar planets by the HATSouth survey. HATS-9b orbits an old (10.8 $\\pm$ 1.5 Gyr) V=13.3 G dwarf star, with a period P = 1.9153 d. The host star has a mass of 1.03 M$_{\\odot}$, radius of 1.503 R$_\\odot$ and effective temperature 5366 $\\pm$ 70 K. The planetary companion has a mass of 0.837 M$_J$, and radius of 1.065 R$_J$ yielding a mean density of 0.85 g cm$^{-3}$ . HATS-10b orbits a V=13.1 G dwarf star, with a period P = 3.3128 d. The host star has a mass of 1.1 M$_\\odot$, radius of 1.11 R$_\\odot$ and effective temperature 5880 $\\pm$ 120 K. The planetary companion has a mass of 0.53 M$_J$, and radius of 0.97 R$_J$ yielding a mean density of 0.7 g cm$^{-3}$ . Both planets are compact in comparison with planets receiving similar irradiation from their host stars, and lie in the nominal coordinates of Field 7 of K2 but only HATS-9b falls on working silicon. Future characterisation of HATS-9b with the exquisite photometric precision of the Kepler telescope may provid...

  6. White Hats Chasing Black Hats: Careers in IT and the Skills Required to Get There. Advisory from Professionals

    Science.gov (United States)

    Fulton, Eric; Lawrence, Cameron; Clouse, Shawn

    2013-01-01

    The aim of this paper is to illuminate the exciting world in which "white hat crackers" operate and to suggest topics that can help prepare students to enter this high-demand field. While currently there is extraordinary demand for graduates to fill these positions that have relatively high starting salaries, employers find it difficult…

  7. Einstein's $R^{\\hat{0} \\hat{0}}$ equation for non-relativistic sources derived from Einstein's inertial motion and the Newtonian law for relative acceleration

    CERN Document Server

    ,

    2016-01-01

    With Einstein's inertial motion (free-falling and non-rotating relative to gyroscopes), geodesics for non-relativistic particles can intersect repeatedly, allowing one to compute the space-time curvature $R^{\\hat{0} \\hat{0}}$ exactly. Einstein's $R^{\\hat{0} \\hat{0}}$ for strong gravitational fields and for relativistic source-matter is identical with the Newtonian expression for the relative radial acceleration of neighboring free-falling test-particles, spherically averaged.--- Einstein's field equations follow from Newtonian experiments, local Lorentz-covariance, and energy-momentum conservation combined with the Bianchi identity.

  8. Experimental studies on the axial crash behavior of aluminum foam-filled hat sections

    Institute of Scientific and Technical Information of China (English)

    WANG Qing-chun; FAN Zi-jie; GUI Liang-jin; WANG Zheng-hong; FU Zi-lai

    2006-01-01

    Drop hammer tests were carried out to study the axial crash behavior of aluminum foam-filled hat sections.First,the axial crash tests of the empty hat sections,aluminum foam and the aluminum foam-filled hat sections were carried out;then,based upon the test results,the axial crash behavior of the aluminum foam-filled hat sections were analyzed.It was found that aluminum foam filling can increase the energy absorption capacities of the hat sections.Compared with the non-filled structures,aluminum foamfilled structures were much more stable and needed less mass to absorb the specified energy.

  9. Schrodinger's Hat: Electromagnetic, acoustic and quantum amplifiers via transformation optics

    CERN Document Server

    Greenleaf, Allan; Lassas, Matti; Uhlmann, Gunther

    2011-01-01

    The advent of transformation optics and metamaterials has made possible devices producing extreme effects on wave propagation. Here we give theoretical designs for devices, Schr\\"odinger hats, acting as invisible concentrators of waves. These exist for any wave phenomenon modeled by either the Helmholtz or Schr\\"odinger equations, e.g., polarized waves in EM, pressure waves in acoustics and matter waves in QM, and occupy one part of a parameter space continuum of wave-manipulating structures which also contains standard transformation optics based cloaks, resonant cloaks and cloaked sensors. For EM and acoustic Schr\\"odinger hats, the resulting centralized wave is a localized excitation. In QM, the result is a new charged quasiparticle, a \\emph{quasmon}, which causes conditional probabilistic illusions. We discuss possible solid state implementations.

  10. HATS-7b: A Hot Super Neptune Transiting a Quiet K Dwarf Star

    Science.gov (United States)

    Bakos, G. Á.; Penev, K.; Bayliss, D.; Hartman, J. D.; Zhou, G.; Brahm, R.; Mancini, L.; de Val-Borro, M.; Bhatti, W.; Jordán, A.; Rabus, M.; Espinoza, N.; Csubry, Z.; Howard, A. W.; Fulton, B. J.; Buchhave, L. A.; Ciceri, S.; Henning, T.; Schmidt, B.; Isaacson, H.; Noyes, R. W.; Marcy, G. W.; Suc, V.; Howe, A. R.; Burrows, A. S.; Lázár, J.; Papp, I.; Sári, P.

    2015-11-01

    We report the discovery by the HATSouth network of HATS-7b, a transiting Super-Neptune with a mass of 0.120 ± 0.012 {M}{{J}}, a radius of {0.563}-0.034+0.046 {R}{{J}}, and an orbital period of 3.1853 days. The host star is a moderately bright (V=13.340\\+/- 0.010 mag, {K}S=10.976\\+/- 0.026 mag) K dwarf star with a mass of 0.849 ± 0.027 {M}⊙ , a radius of {0.815}-0.035+0.049 {R}⊙ , and a metallicity of [{Fe}/{{H}}] =+0.250\\+/- 0.080. The star is photometrically quiet to within the precision of the HATSouth measurements, has low RV jitter, and shows no evidence for chromospheric activity in its spectrum. HATS-7b is the second smallest radius planet discovered by a wide-field ground-based transit survey, and one of only a handful of Neptune-size planets with mass and radius determined to 10% precision. Theoretical modeling of HATS-7b yields a hydrogen-helium fraction of 18 ± 4% (rock-iron core and H2-He envelope), or 9 ± 4% (ice core and H2-He envelope), i.e., it has a composition broadly similar to that of Uranus and Neptune, and very different from that of Saturn, which has 75% of its mass in H2-He. Based on a sample of transiting exoplanets with accurately (Mauna Kea, the MPG 2.2 m and ESO 3.6 m telescopes at the ESO Observatory in La Silla. This paper uses observations obtained with facilities of the Las Cumbres Observatory Global Telescope.

  11. Numerical cubature from Archimedes' hat-box theorem

    OpenAIRE

    Kuperberg, Greg

    2004-01-01

    Archimedes' hat-box theorem states that uniform measure on a sphere projects to uniform measure on an interval. This fact can be used to derive Simpson's rule. We present various constructions of, and lower bounds for, numerical cubature formulas using moment maps as a generalization of Archimedes' theorem. We realize some well-known cubature formulas on simplices as projections of spherical designs. We combine cubature formulas on simplices and tori to make new formulas on spheres. In partic...

  12. Note on contra delta hat g-continuous functions

    Directory of Open Access Journals (Sweden)

    M. Lellis Thivagar

    2012-01-01

    Full Text Available In this paper we introduce and investigate some classes of generalized functions called contra-delta hat g-continuous functions. We obtain several characterizations and some of their properties. Also we investigate its relationship with other types of functions. Finally we introduce two new spaces called deltahat g-Hausdorf spaces and deltahat g-normal spaces and obtain some new results.

  13. Time Dependent \\hat{q} from AdS/CFT

    Science.gov (United States)

    Horowitz, W. A.

    2017-07-01

    We present the first ever AdS/CFT calculation of \\hat{q} for a light quark jet as a function of position or, equivalently, time. Our result does not suffer from the gamma factor blow up of the usual time-independent AdS/CFT heavy quark setup and is qualitatively similar to, but about a factor of 2/3 larger than, the light flavor result from Liu, Rajagopal, and Wiedemann. Our findings can be immediately implemented into any \\hat{q}-based energy loss model. Our \\hat{q} derivation relies on our calculation of the average distance squared, s 2(t), travelled by the endpoint of a string falling in an AdS3-Schwarzschild spacetime. The early time behavior is ballistic, s 2(t) ∼ t 2, but the late time behavior is the usual diffusive Brownian motion, s 2(t) ∼ t. These late time dynamics are universal and depend only on the near-horizon physics, which allows us to generalize our results to arbitrary dimensions and thus make contact with the physics explored by RHIC and LHC. Additionally, we find that AdS/CFT predicts angular ordering for radiation in a medium, just as in vacuum, and in contradistinction to weak-coupling. Finally, our results also imply, sensibly, that AdS/CFT predicts a smooth interpolation between the angular correlations of open heavy flavor and light flavor observables.

  14. Exoplanet HAT-P-11b Secondary Transit Observations

    Science.gov (United States)

    Barry, Richard K., Jr.

    2010-01-01

    We have conducted secondary eclipse observations of exoplanet HAT--11b, recently discovered by proposal G. Bakos and his colleagues. HAT-P-11b is the smallest transiting extrasolar planet yet found and one of only two known exo-Neptunes. We have observed the system at 3.6 microns for a period of 22 hours centered on the anticipated secondary eclipse time, to detect the eclipse and determine its phase. Once the secondary eclipse is located through analysis of the data, we will make a more focused series of observations in both the 3.6 and 4.5 micron bands to fully characterize it. HAT-P-1lb has a period of 4.8878 days, radius of 0.422 RJ, mass of 0.081 MJ and semi major axis 0.053 AU. Measurements of the secondary eclipse will clarify two key issues; 1) the planetary brightness temperature and the nature of its atmosphere, and 2) the eccentricity of its orbit, with implications for its dynamical evolution. A precise determination of the orbit phase for the secondary eclipse will also be of great utility for Kepler observations of this system at visible wavelengths.

  15. Relative photometry of HAT-P-1b occultations

    CERN Document Server

    Béky, Bence; Gilliland, Ronald L; Bakos, Gáspár Á; Winn, Joshua N; Noyes, Robert W; Sasselov, Dimitar D

    2013-01-01

    We present HST STIS observations of two occultations of the transiting exoplanet HAT-P-1b. By measuring the planet to star flux ratio near opposition, we constrain the geometric albedo of the planet, which is strongly linked to its atmospheric temperature gradient. An advantage of HAT-P-1 as a target is its binary companion ADS 16402 A, which provides an excellent photometric reference, simplifying the usual steps in removing instrumental artifacts from HST time-series photometry. We find that without this reference star, we would need to detrend the lightcurve with the time of the exposures as well as the first three powers of HST orbital phase, and this would introduce a strong bias in the results for the albedo. However, with this reference star, we only need to detrend the data with the time of the exposures to achieve the same per-point scatter, therefore we can avoid most of the bias associated with detrending. Our final result is a 2 sigma upper limit of 0.64 for the geometric albedo of HAT-P-1b betwee...

  16. HAT-P-44b, HAT-P-45b, and HAT-P-46b: Three Transiting Hot Jupiters in Possible Multi-Planet Systems

    CERN Document Server

    Hartman, J D; Torres, G; Kovács, G; Johnson, J A; Howard, A W; Marcy, G W; Latham, D W; Bieryla, A; Buchhave, L A; Bhatti, W; Béky, B; Csubry, Z; Penev, K; de Val-Borro, M; Noyes, R W; Fischer, D A; Esquerdo, G A; Everett, M; Szklenár, T; Zhou, G; Bayliss, D; Shporer, A; Fulton, B J; Sanchis-Ojeda, R; Falco, E; Lázár, J; Papp, I; Sári, P

    2013-01-01

    We report the discovery by the HATNet survey of three new transiting extrasolar planets orbiting moderately bright (V=13.2, 12.8 and 11.9) stars. The planets have orbital periods of 4.3012, 3.1290, and 4.4631 days, masses of 0.39, 0.89, and 0.49 Mjup, and radii of 1.28, 1.43, and 1.28 Rjup. The stellar hosts have masses of 0.94, 1.26, and 1.28 Msun. Each system shows significant systematic variations in its residual radial velocities indicating the possible presence of additional components. Based on its Bayesian evidence, the preferred model for HAT-P-44 consists of two planets, including the transiting component, with the outer planet having a period of 220 d and a minimum mass of 1.6 Mjup. Due to aliasing we cannot rule out an alternative solution for the outer planet having a period of 438 d and a minimum mass of 3.7 Mjup. For HAT-P-45 at present there is not enough data to justify the additional free parameters included in a multi-planet model, in this case a single-planet solution is preferred, but the ...

  17. Characterization of new hAT transposable elements in 12 Drosophila genomes.

    Science.gov (United States)

    de Freitas Ortiz, Mauro; Loreto, Elgion Lucio Silva

    2009-01-01

    In silico searches for sequences homologous to hAT elements in 12 Drosophila genomes have allowed us to identify 37 new hAT elements (8 in D. ananassae, 11 in D. mojavensis, 2 in D. sechellia, 1 in D. simulans, 2 in D. virilis, 3 in D. yakuba, 3 in D. persimilis, 1 in D. grimshawi, 5 in D. willistoni and 1 in D. pseudobscura). The size of these elements varies from 2,359 to 4,962 bp and the terminal inverted repeats (TIRs) show lengths ranging from 10 to 24 bp. Several elements show intact transposase ORFs, suggesting that they are active. Conserved amino acid motifs were identified that correspond to those important for transposase activity. These elements are highly variable and phylogenetic analysis showed that they can be clustered into four different families. Incongruencies were observed between the phylogenies of the transposable elements and those of their hosts, suggesting that horizontal transfer may have occurred between some of the species.

  18. The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65.

    Science.gov (United States)

    Kucknoor, Ashwini S; Mundodi, Vasanthakrishna; Alderete, John F

    2007-11-01

    We showed recently that contact of human vaginal epithelial cells (VECs) by Trichomonas vaginalis and incubation with trichomonad proteins in conditioned medium induced expression of VEC genes. We performed 2-D SDS-PAGE followed by MALDI-TOF to identify the major secreted proteins. Based on protein abundance and separation of spots in 2-D gels, 32 major secreted proteins were examined, which gave 19 proteins with accession numbers. These proteins included known secreted cysteine proteinases. In addition, other secreted proteins were enzymes of carbohydrate metabolism, adhesin protein AP65, heat shock proteins, thioredoxin reductase and coronins. We confirmed that the secreted trichomonad proteins induced expression of VEC genes, including interleukin 8 (IL-8), COX-2 and fibronectin. Purified AP65 added to VECs had a pronounced effect only on IL-8 gene expression, which was inhibited in the presence of 12G4 monoclonal antibody to AP65. Moreover, AP65 expressed episomally within epithelial cells was found to enhance the expression of IL-8 and COX-2. This may be the first report of analysis of the secreted proteins of T. vaginalis and of the host epithelial cell response to these proteins and to the prominent adhesin AP65.

  19. A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair

    Directory of Open Access Journals (Sweden)

    Leclerc Xavier

    2009-04-01

    Full Text Available Abstract Background Current strategies for gene therapy of inherited diseases consist in adding functional copies of the gene that is defective. An attractive alternative to these approaches would be to correct the endogenous mutated gene in the affected individual. This study presents a quantitative comparison of the repair efficiency using different forms of donor nucleic acids, including synthetic DNA oligonucleotides, double stranded DNA fragments with sizes ranging from 200 to 2200 bp and sequences carried by a recombinant adeno-associated virus (rAAV-1. Evaluation of each gene repair strategy was carried out using two different reporter systems, a mutated eGFP gene or a dual construct with a functional eGFP and an inactive luciferase gene, in several different cell systems. Gene targeting events were scored either following transient co-transfection of reporter plasmids and donor DNAs, or in a system where a reporter construct was stably integrated into the chromosome. Results In both episomal and chromosomal assays, DNA fragments were more efficient at gene repair than oligonucleotides or rAAV-1. Furthermore, the gene targeting frequency could be significantly increased by using DNA repair stimulating drugs such as doxorubicin and phleomycin. Conclusion Our results show that it is possible to obtain repair frequencies of 1% of the transfected cell population under optimized transfection protocols when cells were pretreated with phleomycin using rAAV-1 and dsDNA fragments.

  20. HAT-P-44b, HAT-P-45b, and HAT-P-46b: Three Transiting Hot Jupiters in Possible Multi-planet Systems

    Science.gov (United States)

    Hartman, J. D.; Bakos, G. Á.; Torres, G.; Kovács, G.; Johnson, J. A.; Howard, A. W.; Marcy, G. W.; Latham, D. W.; Bieryla, A.; Buchhave, L. A.; Bhatti, W.; Béky, B.; Csubry, Z.; Penev, K.; de Val-Borro, M.; Noyes, R. W.; Fischer, D. A.; Esquerdo, G. A.; Everett, M.; Szklenár, T.; Zhou, G.; Bayliss, D.; Shporer, A.; Fulton, B. J.; Sanchis-Ojeda, R.; Falco, E.; Lázár, J.; Papp, I.; Sári, P.

    2014-06-01

    We report the discovery by the HATNet survey of three new transiting extrasolar planets orbiting moderately bright (V = 13.2, 12.8, and 11.9) stars. The planets have orbital periods of 4.3012, 3.1290, and 4.4631 days, masses of 0.35, 0.89, and 0.49 M J, and radii of 1.24, 1.43, and 1.28 R J. The stellar hosts have masses of 0.94, 1.26, and 1.28 M ⊙. Each system shows significant systematic variations in its residual radial velocities, indicating the possible presence of additional components. Based on its Bayesian evidence, the preferred model for HAT-P-44 consists of two planets, including the transiting component, with the outer planet having a period of 872 days, eccentricity of 0.494 ± 0.081, and a minimum mass of 4.0 M J. Due to aliasing we cannot rule out alternative solutions for the outer planet having a period of 220 days or 438 days. For HAT-P-45, at present there is not enough data to justify the additional free parameters included in a multi-planet model; in this case a single-planet solution is preferred, but the required jitter of 22.5 ± 6.3 m s-1 is relatively high for a star of this type. For HAT-P-46 the preferred solution includes a second planet having a period of 78 days and a minimum mass of 2.0 M J, however the preference for this model over a single-planet model is not very strong. While substantial uncertainties remain as to the presence and/or properties of the outer planetary companions in these systems, the inner transiting planets are well characterized with measured properties that are fairly robust against changes in the assumed models for the outer planets. Continued radial velocity monitoring is necessary to fully characterize these three planetary systems, the properties of which may have important implications for understanding the formation of hot Jupiters. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck

  1. HAT-P-44b, HAT-P-45b, AND HAT-P-46b: Three transiting hot Jupiters in possible multi-planet systems

    Energy Technology Data Exchange (ETDEWEB)

    Hartman, J. D.; Bakos, G. Á.; Bhatti, W.; Csubry, Z.; Penev, K.; De Val-Borro, M. [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08544 (United States); Torres, G.; Latham, D. W.; Bieryla, A.; Béky, B.; Noyes, R. W.; Esquerdo, G. A. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Kovács, G. [Konkoly Observatory, Budapest (Hungary); Johnson, J. A. [Department of Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Howard, A. W. [Institute for Astronomy, University of Hawaii, Honolulu, HI 96822 (United States); Marcy, G. W. [Department of Astronomy, University of California, Berkeley, CA 94720 (United States); Buchhave, L. A. [Niels Bohr Institute, Copenhagen University, DK-2100 Copenhagen (Denmark); Fischer, D. A. [Department of Astronomy, Yale University, New Haven, CT 06520-8101 (United States); Everett, M. [National Optical Astronomy Observatory, 950 North Cherry Avenue, Tucson, AZ 85719 (United States); Szklenár, T., E-mail: jhartman@astro.princeton.edu [Hungarian Astronomical Association, 1461 Budapest (Hungary); and others

    2014-06-01

    We report the discovery by the HATNet survey of three new transiting extrasolar planets orbiting moderately bright (V = 13.2, 12.8, and 11.9) stars. The planets have orbital periods of 4.3012, 3.1290, and 4.4631 days, masses of 0.35, 0.89, and 0.49 M {sub J}, and radii of 1.24, 1.43, and 1.28 R {sub J}. The stellar hosts have masses of 0.94, 1.26, and 1.28 M {sub ☉}. Each system shows significant systematic variations in its residual radial velocities, indicating the possible presence of additional components. Based on its Bayesian evidence, the preferred model for HAT-P-44 consists of two planets, including the transiting component, with the outer planet having a period of 872 days, eccentricity of 0.494 ± 0.081, and a minimum mass of 4.0 M {sub J}. Due to aliasing we cannot rule out alternative solutions for the outer planet having a period of 220 days or 438 days. For HAT-P-45, at present there is not enough data to justify the additional free parameters included in a multi-planet model; in this case a single-planet solution is preferred, but the required jitter of 22.5 ± 6.3 m s{sup –1} is relatively high for a star of this type. For HAT-P-46 the preferred solution includes a second planet having a period of 78 days and a minimum mass of 2.0 M {sub J}, however the preference for this model over a single-planet model is not very strong. While substantial uncertainties remain as to the presence and/or properties of the outer planetary companions in these systems, the inner transiting planets are well characterized with measured properties that are fairly robust against changes in the assumed models for the outer planets. Continued radial velocity monitoring is necessary to fully characterize these three planetary systems, the properties of which may have important implications for understanding the formation of hot Jupiters.

  2. Lightning as a possible source of the radio emission on HAT-P-11b

    CERN Document Server

    Hodosán, Gabriella; Helling, Christiane

    2016-01-01

    Lightning induced radio emission has been observed on Solar System planets. There have been many attempts to observe exoplanets in the radio wavelength, however, no unequivocal detection has been reported. Lecavelier des Etangs et al. (2013, A&A, 552, A65) carried out radio transit observations of the exoplanet HAT-P-11b, and suggested that a small part of the radio flux can be attributed to the planet. In the current letter, we assume that this signal is real, and study if this radio emission could be caused by lightning in the atmosphere of the planet. We find that a lightning storm with 530 times larger flash densities than the Earth-storms with the largest lightning activity is needed to produce the observed signal from HAT-P-11b. The optical counterpart would nevertheless be undetectable with current technology. We show that HCN produced by lightning chemistry of such thunderstorms is observable 2-3 years after the storm, which produces signatures in the L ($3.0 \\mu{\\rm m}-4.0 \\mu$m) and N ($7.5 \\mu{...

  3. Kepler and Ground-based Transits of the Exo-Neptune HAT-P-11b

    CERN Document Server

    Deming, Drake; Jackson, Brian; Peterson, Steven W; Agol, Eric; Knutson, Heather A; Jennings, Donald E; Haase, Flynn; Bays, Kevin

    2011-01-01

    We analyze 26 archival Kepler transits of the exo-Neptune HAT-P-11b, supplemented by ground-based transits observed in the blue (B-band) and near-IR (J-band). Both the planet and host star are smaller than previously believed; our analysis yields Rp=4.31 +/-0.06 Earth-radii, and Rs = 0.683 +/-0.009 solar radii, both about 3-sigma smaller than the discovery values. Our ground-based transit data at wavelengths bracketing the Kepler bandpass serve to check the wavelength dependence of stellar limb darkening, and the J-band transit provides a precise and independent constraint on the transit duration. Both the limb darkening and transit duration from our ground-based data are consistent with the new Kepler values for the system parameters. Our smaller radius for the planet implies that its gaseous envelope can be less extensive than previously believed, being very similar to the H-He envelope of GJ436b and Kepler-4b. HAT-P-11 is an active star, and signatures of star spot crossings are ubiquitous in the Kepler tr...

  4. Discovery of the secondary eclipse of HAT-P-11 b

    CERN Document Server

    Huber, K F; Schmitt, J H M M

    2016-01-01

    We report the detection of the secondary eclipse of HAT-P-11 b, a Neptune-sized planet orbiting an active K4 dwarf. Using all available short-cadence data of the Kepler mission, we derive refined planetary ephemeris increasing their precision by more than an order of magnitude. Our simultaneous primary and secondary transit modeling results in improved transit and orbital parameters. In particular, the precise timing of the secondary eclipse allows to pin down the orbital eccentricity to $0.26459_{-0.00048}^{+0.00069}$. The secondary eclipse depth of $6.09_{-1.11}^{+1.12}$ ppm corresponds to a $5.5\\sigma$ detection and results in a geometric albedo of $0.39\\pm0.07$ for HAT-P-11 b, close to Neptune's value, which may indicate further resemblances between these two bodies. Due to the substantial orbital eccentricity, the planetary equilibrium temperature is expected to change significantly with orbital position and ought to vary between $630^\\circ$ K and $950^\\circ$ K, depending on the details of heat redistrib...

  5. Synthesis of isothiazol-3-one derivatives as inhibitors of histone acetyltransferases (HATs).

    Science.gov (United States)

    Gorsuch, Stephen; Bavetsias, Vassilios; Rowlands, Martin G; Aherne, G Wynne; Workman, Paul; Jarman, Michael; McDonald, Edward

    2009-01-15

    High-throughput screening led to the identification of isothiazolones 1 and 2 as inhibitors of histone acetyltransferase (HAT) with IC50s of 3 microM and 5 microM, respectively. Analogues of these hit compounds with variations of the N-phenyl group, and with variety of substituents at C-4, C-5 of the thiazolone ring, were prepared and assayed for inhibition of the HAT enzyme PCAF. Potency is modestly favoured when the N-aryl group is electron deficient (4-pyridyl derivative 10 has IC(50)=1.5 microM); alkyl substitution at C-4 has little effect, whilst similar substitution at C-5 causes a significant drop in potency. The ring-fused compound 38 has activity (IC(50)=6.1 microM) to encourage further exploration of this bicyclic structure. The foregoing SAR is consistent with an inhibitory mechanism involving cleavage of the S-N bond of the isothiazolone ring by a catalytically important thiol residue.

  6. USING SIX THINKING HATS AS A TOOL FOR LATERAL THINKING IN ORGANIZATIONAL PROBLEM SOLVING

    OpenAIRE

    Dr. P. S. Aithal; Dr. P. M. Suresh Kumar

    2016-01-01

    Six thinking hats is recently introduced technique which outlines different thinking styles required by an individual while analysing a given problem in an effective way. The technique correlates different thinking styles used in a systematic problem-solving procedure with different coloured hats. Alternately, by conceptualizing each type of hat, the person focuses on the style of thinking associated with each colour so that the problem can be analysed from different angles and frame of refer...

  7. General survey of hAT transposon superfamily with highlight on hobo element in Drosophila.

    Science.gov (United States)

    Ladevèze, Véronique; Chaminade, Nicole; Lemeunier, Françoise; Periquet, Georges; Aulard, Sylvie

    2012-09-01

    The hAT transposons, very abundant in all kingdoms, have a common evolutionary origin probably predating the plant-fungi-animal divergence. In this paper we present their general characteristics. Members of this superfamily belong to Class II transposable elements. hAT elements share transposase, short terminal inverted repeats and eight base-pairs duplication of genomic target. We focus on hAT elements in Drosophila, especially hobo. Its distribution, dynamics and impact on genome restructuring in laboratory strains as well as in natural populations are reported. Finally, the evolutionary history of hAT elements, their domestication and use as transgenic tools are discussed.

  8. FACTORS & ELEMENTAL ANALYSIS OF SIX THINKING HATS TECHNIQUE USING ABCD FRAMEWORK

    OpenAIRE

    Dr. P. S. Aithal; V. T. Shailashree; Dr. P. M. Suresh Kumar

    2017-01-01

    De Bono's Six Thinking Hats technique suggests different types of thinking corresponding to six thinking roles for the analyst, associated with hats of six different colors. The technique correlates different thinking styles used in a systematic problem solving procedure with different coloured hats. Alternately, by conceptualizing each type of hat, the person focuses on the style of thinking associated with each colour so that the problem can be analysed from different angles and frame of re...

  9. Wie viel Kapazität hat ein Mobilfunknetz?

    Science.gov (United States)

    Eisenblätter, Andreas; Geerdes, Hans-Florian

    Wer hat das noch nicht erlebt? Der Akku ist aufgeladen und reichlich Guthaben vorhanden, doch das Telefonieren mit dem Handy klappt trotzdem nicht. Meist liegt das daran, dass man sich in einem Funkloch befindet. Aber manchmal ist auch das Netz überlastet. Anders gesagt: das Funknetz des Mobilfunkanbieters, das viele Antennen auf Hausdächern oder Masten umfasst, bietet entweder nicht genügend Abdeckung oder nicht genügend Kapazität. Genau das will der Anbieter natürlich vermeiden. Mathematik hilft, diese Herausforderungen mit einer guten Planung des Mobilfunknetzes zu bewältigen.

  10. HST hot Jupiter Transmission Spectral Survey: A detection of Na and strong optical absorption in HAT-P-1b

    CERN Document Server

    Nikolov, N; Pont, F; Burrows, A S; Fortney, J J; Ballester, G E; Evans, T M; Huitson, C M; Wakeford, H R; Wilson, P A; Aigrain, S; Deming, D; Gibson, N P; Henry, G W; Knutson, H; Etangs, A Lecavelier des; Showman, A P; Vidal-Madjar, A; Zahnle, K

    2013-01-01

    We present an optical to near-infrared transmission spectrum of the hot Jupiter HAT-P-1b, based on HST observations, covering the spectral regime from 0.29 to 1.027{\\mu}m with STIS, which is coupled with a recent WFC3 transit (1.087 to 1.687{\\mu}m). We derive refined physical parameters of the HAT-P-1 system, including an improved orbital ephemeris. The transmission spectrum shows a strong absorption signature shortward of 0.55{\\mu}m, with a strong blueward slope into the near-ultraviolet. We detect atmospheric sodium absorption at a 3.3{\\sigma} significance level, but find no evidence for the potassium feature. The red data implies a marginally flat spectrum with a tentative absorption enhancement at wavelength longer than ~0.85{\\mu}m. The STIS and WFC3 spectra differ significantly in absolute radius level (4.3 +/- 1.6 pressure scale heights), implying strong optical absorption in the atmosphere of HAT-P-1b. The optical to near-infrared difference cannot be explained by stellar activity, as simulta- neous st...

  11. Remedial Action Plan for the Codisposal and Stabilization of the Monument Valley and Mexican Hat Uranium Mill Tailings at Mexican Hat, Utah

    OpenAIRE

    Uranium Mill Tailings Remedial Action Project Office, Albuquerque Operations Office, Department of Energy

    1993-01-01

    The Mexican Hat tailings site is in the San Juan County, Utah, two road miles southwest of the town of Mexican Hat on the Navajo Reservation. The Navajo community of Halchita is approximately 0.5 mile southwest of the site. The mill at the Mexican Hat site was operated from 1957 to 1965 by Texas-Zinc Minerals Corporation and the Atlas Corporation. Originally, two irregularly shaped tailings piles were located in the northeastern portion of the site. They occupied approximately 69 acres of...

  12. Kepler and Ground-Based Transits of the exo-Neptune HAT-P-11b

    Science.gov (United States)

    Deming, Drake; Sada, Pedro V.; Jackson, Brian; Peterson, Steven W.; Agol, Eric; Knutson, Heather A.; Jennings, Donald E.; Haase, Plynn; Bays, Kevin

    2011-01-01

    We analyze 26 archival Kepler transits of the exo-Neptune HAT-P-11b, supplemented by ground-based transits observed in the blue (B band) and near-IR (J band). Both the planet and host star are smaller than previously believed; our analysis yields Rp = 4.31 R xor 0.06 R xor and Rs = 0.683 R solar mass 0.009 R solar mass, both about 3 sigma smaller than the discovery values. Our ground-based transit data at wavelengths bracketing the Kepler bandpass serve to check the wavelength dependence of stellar limb darkening, and the J-band transit provides a precise and independent constraint on the transit duration. Both the limb darkening and transit duration from our ground-based data are consistent with the new Kepler values for the system parameters. Our smaller radius for the planet implies that its gaseous envelope can be less extensive than previously believed, being very similar to the H-He envelope of GJ 436b and Kepler-4b. HAT-P-11 is an active star, and signatures of star spot crossings are ubiquitous in the Kepler transit data. We develop and apply a methodology to correct the planetary radius for the presence of both crossed and uncrossed star spots. Star spot crossings are concentrated at phases 0.002 and +0.006. This is consistent with inferences from Rossiter-McLaughlin measurements that the planet transits nearly perpendicular to the stellar equator. We identify the dominant phases of star spot crossings with active latitudes on the star, and infer that the stellar rotational pole is inclined at about 12 deg 5 deg to the plane of the sky. We point out that precise transit measurements over long durations could in principle allow us to construct a stellar Butterfly diagram to probe the cyclic evolution of magnetic activity on this active K-dwarf star.

  13. The History of SETI at the Hat Creek Radio Observatory

    Science.gov (United States)

    Tarter, J.

    2006-12-01

    Since the first SETI search in 1960, observations have encountered an exponentially growing problem with radio frequency interference (RFI) generated by our own communication, entertainment, and military technologies. The signal processing equipment that is used for SETI has gotten much faster and more capable, yet the fraction of the possible search space that has been explored remains very small. More than 100 searches have been reported in the literature. Tarter (2001) has summarized the various search strategies and the SETI Institute maintains an updated search archive at http://www.seti.org/searcharchive. The Allen Telescope Array (ATA) at Hat Creek Radio Observatory will be the first instrument designed with SETI as a goal, and its speed and flexibility will permit a significant exploration of our local region of the Milky Way Galaxy, targeting ˜1 million stars for weak signals, as well as surveying for stronger signals from ˜40 billion distant stars, located in the direction of the galactic center and the surrounding 20 square degrees. Just as Jack Welch has been responsible for many of the innovations in the ATA and the SETI observations it will soon undertake, he has been the key to enabling SETI at the Hat Creek Radio Observatory for the past three decades.

  14. A Bayesian Atmospheric Retrieval Performed on HAT-P-16b and WASP-11b/HAT-P-10b

    Science.gov (United States)

    McIntyre, Kathleen J.; Harrington, Joseph; Challener, Ryan C.; Hardin, Matthew Ryan; Bowman, Oliver Oliver; Foster, Andrew S. D.; Lenius, Maria; Hartman, Joel D.; Bakos, Gaspar; Blecic, Jasmina; Cubillos, Patricio; Ariston Hardy, Ryan; Cameron, Andrew

    2016-10-01

    HAT-P-16b is a hot (equilibrium temperature 1626 ± 40 K, assuming zero Bond albedo and efficient energy redistribution), 4.19 ± 0.09 Jupiter-mass exoplanet orbiting an F8 star every 2.775960 ± 0.000003 days (Buchhave et al 2010). WASP-11b/HAT-P-10b is a cooler (1020 ± 17 K), 0.487 ± 0.018 Jupiter-mass exoplanet orbiting a K3 star every 3.7224747 ± 0.0000065 days (Bakos et al. 2009, co-discovered by West et al. 2008). We observed secondary eclipses of both planets using the 3.6 μm and 4.5 μm channels of the Spitzer Space Telescope's Infrared Array Camera (program ID 60003). We applied our Bayesian Atmospheric Radiative Transfer (BART) code to constrain the temperature-pressure profiles and atmospheric molecular abundances of the two planets. Spitzer is operated by the Jet Propulsion Laboratory, California Institute of Technology, under a contract with NASA. This work was supported by NASA Planetary Atmospheres grant NNX12AI69G and NASA Astrophysics Data Analysis Program grant NNX13AF38G.

  15. Home Automated Telemanagement (HAT System to Facilitate Self-Care of Patients with Chronic Diseases

    Directory of Open Access Journals (Sweden)

    Joseph Finkelstein

    2003-06-01

    Full Text Available Successful patient self-management requires a multidisciplinary approach that includes regular patient assessment, disease-specific education, control of medication adherence, implementation of health behavior change models and social support. Existing systems for computer-assisted disease management do not provide this multidisciplinary patient support and do not address treatment compliance issues. We developed the Home Automated Telemanagement (HAT system for patients with different chronic health conditions to facilitate their self-care. The HAT system consists of a home unit, HAT server, and clinician units. Patients at home use a palmtop or a laptop connected with a disease monitor on a regular basis. Each HAT session consists of self-testing, feedback, and educational components. The self-reported symptom data and objective results obtained from disease-specific sensors are automatically sent from patient homes to the HAT server in the hospital. Any web-enabled device can serve as a clinician unit to review patient results. The HAT system monitors self-testing results and patient compliance. The HAT system has been implemented and tested in patients receiving anticoagulation therapy, patients with asthma, COPD and other health conditions. Evaluation results indicated high level of acceptance of the HAT system by the patients and that the system has a positive impact on main clinical outcomes and patient satisfaction with medical care.

  16. Heroin-assisted treatment (HAT) a decade later : A brief update on science and politics

    NARCIS (Netherlands)

    Fischer, Benedikt; Oviedo-Joekes, Eugenia; Blanken, Peter; Haasen, Christian; Rehm, Juergen; Schechter, Martin T.; Strang, John; van den Brink, Wim

    2007-01-01

    Since the initial Swiss heroin-assisted treatment (HAT) study conducted in the mid-1990s, several other jurisdictions in Europe and North America have implemented HAT trials. All of these studies embrace the same goal-investigating the utility of medical heroin prescribing for problematic opioid use

  17. Not Just Hats Anymore: Binomial Inversion and the Problem of Multiple Coincidences

    Science.gov (United States)

    Hathout, Leith

    2007-01-01

    The well-known "hats" problem, in which a number of people enter a restaurant and check their hats, and then receive them back at random, is often used to illustrate the concept of derangements, that is, permutations with no fixed points. In this paper, the problem is extended to multiple items of clothing, and a general solution to the problem of…

  18. Free Field Realization of Quantum Superalgebra $U_q(\\hat{{sl}}(N|1))$

    CERN Document Server

    Kojima, Takeo

    2011-01-01

    We construct a free field realization of the quantum superalgebra $U_q(\\hat{sl}(N|1))$ for an arbitrary level $k$, that we call $q$-Wakimoto realization. Using the dressing deformation, we construct a free field realization of the the elliptic deformed superalgebra $U_{q,p}(\\hat{sl}(N|1))$ for an arbitrary level $k$.

  19. Giant Paperclip Necklaces, Soup-Can Rings and Cherry-Pie Hats

    Science.gov (United States)

    Winters, Laurel A.

    2011-01-01

    In this article, the author describes an art project inspired by the wearable sculpture art created by artist Marjorie Schick. Students used wallpaper paste and newspapers to create papier-mache for a mountain hat, a cherry-pie mask/hat, a "dress" shoe and a Cubistic mask. Cardboard was used in many of these things, in addition to being used as…

  20. The man who mistook his wife for a hat

    CERN Document Server

    Sacks, Oliver

    2011-01-01

    Oliver Sacks’s The Man Who Mistook His Wife for a Hat tells the stories of individuals afflicted with fantastic perceptual and intellectual aberrations: patients who have lost their memories and with them the greater part of their pasts; who are no longer able to recognize people and common objects; who are stricken with violent tics and grimaces or who shout involuntary obscenities; whose limbs have become alien; who have been dismissed as retarded yet are gifted with uncanny artistic or mathematical talents. If inconceivably strange, these brilliant tales remain, in Dr. Sacks’s splendid and sympathetic telling, deeply human. They are studies of life struggling against incredible adversity, and they enable us to enter the world of the neurologically impaired, to imagine with our hearts what it must be to live and feel as they do. A great healer, Sacks never loses sight of medicine’s ultimate responsibility: “the suffering, afflicted, fighting human subject.”

  1. Supernova 1987 A - The nebular loops and 'Napoleon's Hat'

    Science.gov (United States)

    Wang, L.; Wampler, E. J.

    1992-08-01

    We discuss observations of the circumstellar environment of SN 1987A that were obtained between August 1989 and January 1992 at ESO's New Technology Telescope. We find that the angular dimensions of the two nebular loops (Wampler et al., 1990) have not changed during this period. Therefore these loops are confined to a small region. The expansion velocity of the loops is less than about 40 km/s if the loops expanded with a uniform velocity from a common origin. This structure and velocity is hard to reproduce with existing wind interaction models. Our observations further suggest that the Napoleon's Hat nebula does not originate from the general background LMC dust, but from a bow shock dust whose origins are closely related to the stellar winds from the progenitor star of SN 1987A.

  2. Secondary Eclipses of HAT-P-13b

    Science.gov (United States)

    Hardy, Ryan A.; Harrington, Joseph; Hardin, Matthew R.; Madhusudhan, Nikku; Loredo, Thomas J.; Challener, Ryan C.; Foster, Andrew S. D.; Cubillos, Patricio E.; Blecic, Jasmina

    2017-02-01

    We present Spitzer secondary-eclipse observations of the hot Jupiter HAT-P-13 b in the 3.6 and 4.5 μm bands. HAT-P-13 b inhabits a two-planet system with a configuration that enables constraints on the planet’s second Love number, {k}2, from precise eccentricity measurements, which in turn constrains models of the planet’s interior structure. We exploit the direct measurements of e\\cos ω from our secondary-eclipse data and combine them with previously published radial velocity data to generate a refined model of the planet’s orbit and thus an improved estimate on the possible interval for {k}2. We report eclipse phases of 0.49154+/- 0.00080 and 0.49711+/- 0.00083 and corresponding e\\cos ω estimates of -0.0136+/- 0.0013 and -0.0048+/- 0.0013. Under the assumptions of previous work, our estimate of {k}2 of 0.81 ± 0.10 is consistent with the lower extremes of possible core masses found by previous models, including models with no solid core. This anomalous result challenges both interior models and the dynamical assumptions that enable them, including the essential assumption of apsidal alignment. We also report eclipse depths of 0.081% ± 0.008% in the 3.6 μm channel and 0.088% ± 0.028% in the 4.5 μm channel. These photometric results are non-uniquely consistent with solar-abundance composition without any thermal inversion.

  3. Enhanced Generation of Integration-free iPSCs from Human Adult Peripheral Blood Mononuclear Cells with an Optimal Combination of Episomal Vectors

    Directory of Open Access Journals (Sweden)

    Wei Wen

    2016-06-01

    Full Text Available We previously reported the generation of integration-free induced pluripotent stem cells from adult peripheral blood (PB with an improved episomal vector (EV system, which uses the spleen focus-forming virus U3 promoter and an extra factor BCL-XL (B. Here we show an ∼100-fold increase in efficiency by optimizing the vector combination. The two most critical factors are: (1 equimolar expression of OCT4 (O and SOX2 (S, by using a 2A linker; (2 a higher and gradual increase in the MYC (M to KLF4 (K ratio during the course of reprogramming, by using two individual vectors to express M and K instead of one. The combination of EV plasmids (OS + M + K + B is comparable with Sendai virus in reprogramming efficiency but at a fraction of the cost. The generated iPSCs are indistinguishable from those from our previous approach in pluripotency and phenotype. This improvement lays the foundation for broad applications of episomal vectors in PB reprogramming.

  4. Enhanced Generation of Integration-free iPSCs from Human Adult Peripheral Blood Mononuclear Cells with an Optimal Combination of Episomal Vectors.

    Science.gov (United States)

    Wen, Wei; Zhang, Jian-Ping; Xu, Jing; Su, Ruijun Jeanna; Neises, Amanda; Ji, Guang-Zhen; Yuan, Weiping; Cheng, Tao; Zhang, Xiao-Bing

    2016-06-14

    We previously reported the generation of integration-free induced pluripotent stem cells from adult peripheral blood (PB) with an improved episomal vector (EV) system, which uses the spleen focus-forming virus U3 promoter and an extra factor BCL-XL (B). Here we show an ∼100-fold increase in efficiency by optimizing the vector combination. The two most critical factors are: (1) equimolar expression of OCT4 (O) and SOX2 (S), by using a 2A linker; (2) a higher and gradual increase in the MYC (M) to KLF4 (K) ratio during the course of reprogramming, by using two individual vectors to express M and K instead of one. The combination of EV plasmids (OS + M + K + B) is comparable with Sendai virus in reprogramming efficiency but at a fraction of the cost. The generated iPSCs are indistinguishable from those from our previous approach in pluripotency and phenotype. This improvement lays the foundation for broad applications of episomal vectors in PB reprogramming.

  5. Dialing the Love Number of Hot Jupiter HAT-P-13b

    Science.gov (United States)

    Buhler, Peter

    2015-05-01

    HAT-P-13b is Jupiter-mass transiting planet in a 0.04 AU orbit around its host star. It has an outer companion, HAT-P-13c, with a minimum mass of 14.7 MJup in a highly eccentric 1.2 AU orbit. These two companions form an isolated dynamical system with their host star [1]. The nature of this system allows the two bodies to settle into a fixed eccentricity state where the eccentricity of HAT-P-13b is directly related to its oblateness as described by the Love number, k2 [2]. In order to constrain the eccentricity, and therefore k2, of HAT-P-13b, we use the Spitzer Space Telescope to measure the timing of its secondary eclipses at 3.6 and 4.5 μm. We then simultaneously fit our secondary eclipse data in conjunction with previously measured radial velocity and transit data. Finally, we apply the fact that, if the orbits of HAT-P-13b and HAT-P-13c are coplanar, then their apsides are aligned [3]. The apsidal orientation of HAT-P-13c is much better constrained because of its high eccentricity, which helps break the degeneracy between the eccentricity and apsidal orientation in interpreting the measured secondary eclipse time. Our analysis allows us to measure the eccentricity of HAT-P-13b’s orbit with a precision approximately ten times better than that of previously published values, in the coplanar case, and allows us to place the first meaningful constraints on the core mass of HAT-P-13b.[1] Becker & Batygin 2013, ApJ 778, 100 [2] Wu & Goldreich 2002, ApJ 564, 1024 [3] Batygin+ 2009, ApJ 704, L49

  6. HATS-11b AND HATS-12b: Two Transiting Hot Jupiters Orbiting Subsolar Metallicity Stars Selected for the K2 Campaign 7

    Science.gov (United States)

    Rabus, M.; Jordán, A.; Hartman, J. D.; Bakos, G. Á.; Espinoza, N.; Brahm, R.; Penev, K.; Ciceri, S.; Zhou, G.; Bayliss, D.; Mancini, L.; Bhatti, W.; de Val-Borro, M.; Csbury, Z.; Sato, B.; Tan, T.-G.; Henning, T.; Schmidt, B.; Bento, J.; Suc, V.; Noyes, R.; Lázár, J.; Papp, I.; Sári, P.

    2016-10-01

    We report the discovery of two transiting extrasolar planets from the HATSouth survey. HATS-11, a V = 14.1 G0-star shows a periodic 12.9 mmag dip in its light curve every 3.6192 days and a radial velocity variation consistent with a Keplerian orbit. HATS-11 has a mass of 1.000+/- 0.060 {M}⊙ , a radius of 1.444+/- 0.057 {R}⊙ and an effective temperature of 6060+/- 150 K, while its companion is a 0.85+/- 0.12 {M}{{J}}, 1.510+/- 0.078 {R}{{J}} planet in a circular orbit. HATS-12 shows a periodic 5.1 mmag flux decrease every 3.1428 days and Keplerian RV variations around a V = 12.8 F-star. HATS-12 has a mass of 1.489+/- 0.071 {M}⊙ , a radius of 2.21+/- 0.21 {R}⊙ , and an effective temperature of 6408+/- 75 K. For HATS-12b, our measurements indicate that this is a 2.38+/- 0.11 {M}{{J}}, 1.35+/- 0.17 {R}{{J}} planet in a circular orbit. Both host stars show subsolar metallicities of -0.390+/- 0.060 dex and -0.100+/- 0.040 dex, respectively, and are (slightly) evolved stars. In fact, HATS-11 is among the most metal-poor and, HATS-12, with a {log}{g}\\star of 3.923+/- 0.065, is among the most evolved stars hosting a hot-Jupiter planet. Importantly, HATS-11 and HATS-12 have been observed in long cadence by Kepler as part of K2 campaign 7 (EPIC216414930 and EPIC218131080 respectively). The HATSouth network is operated by a collaboration consisting of Princeton University (PU), the Max Planck Institute für Astronomie (MPIA), the Australian National University (ANU), and the Pontificia Universidad Católica de Chile (PUC). The station at Las Campanas Observatory (LCO) of the Carnegie Institute is operated by PU in conjunction with PUC, the station at the High Energy Spectroscopic Survey (H.E.S.S.) site is operated in conjunction with MPIA, and the station at Siding Spring Observatory (SSO) is operated jointly with ANU. Based in part on data collected at the Subaru Telescope, which is operated by the National Astronomical Observatory of Japan. Based in part on

  7. HATS-11b and HATS-12b: Two transiting Hot Jupiters orbiting sub-solar metallicity stars selected for the K2 Campaign 7

    CERN Document Server

    Rabus, M; Hartman, J D; Bakos, G Á; Espinoza, N; Brahm, R; Penev, K; Ciceri, S; Zhou, G; Bayliss, D; Mancini, L; Bhatti, W; de Val-Borro, M; Csbury, Z; Sato, B; Tan, T -G; Henning, T; Schmidt, B; Bento, J; Suc, V; Noyes, R; Lázár, J; Papp, I; Sári, P

    2016-01-01

    We report the discovery of two transiting extrasolar planets from the HATSouth survey. HATS-11, a V=14.1 G0-star shows a periodic 12.9 mmag dip in its light curve every 3.6192 days and a radial velocity variation consistent with a Keplerian orbit. HATS-11 has a mass of 1.000 $\\pm$ 0.060 M$_{\\odot}$, a radius of 1.444 $\\pm$ 0.057 M$_{\\odot}$ and an effective temperature of 6060 $\\pm$ 150 K, while its companion is a 0.85 $\\pm$ 0.12 M$_J$, 1.510 $\\pm$ 0.078 R$_J$ planet in a circular orbit. HATS-12 shows a periodic 5.1 mmag flux decrease every 3.1428 days and Keplerian RV variations around a V=12.8 F-star. HATS-12 has a mass of 1.489 $\\pm$ 0.071 M$_{\\odot}$, a radius of 2.21 $\\pm$ 0.21 R$_{\\odot}$, and an effective temperature of 6408 $\\pm$ 75 K. For HATS-12, our measurements indicate that this is a 2.38 $\\pm$ 0.11 M$_J$, 1.35 $\\pm$ 0.17 R$_J$ planet in a circular orbit. Both host stars show sub-solar metallicity of -0.390 $\\pm$ 0.060 dex and -0.100 $\\pm$ 0.040 dex, respectively and are (slightly) evolved stars....

  8. The Effect Of Using The Creative Drama Method and The Six Thinking Hat Technique On Student Success and Attidudes In Eighth-Grade Revolution History and Kemalism Lesson

    Directory of Open Access Journals (Sweden)

    Ali ALTIKULAÇ

    2010-12-01

    Full Text Available In this research, it was aimed to compose the activities about how creative drama teaching method and six thinking hats teaching technique can be used in “Lausanne Peace Treaty” subject in Turkish Republic Revolution History and Kemalism lesson; describe and research whether the composed activities increase the students’ learning levels or not. For this aim, the Lausanne Peace Treaty-related activities were applied on an experiment group and traditional teaching techniques were applied on a checking group. In this study, which is an experimental and descriptive, it was attempted to show the use of creative drama teaching method and six thinking hats teaching technique in Turkish Republic Revolution History and Kemalism lesson and an answer was searched for the question “Is there any meaningful difference between the learning level and attitudes of experiment group and checking group?”

  9. Heroin-assisted treatment (HAT) a decade later: a brief update on science and politics.

    Science.gov (United States)

    Fischer, Benedikt; Oviedo-Joekes, Eugenia; Blanken, Peter; Haasen, Christian; Rehm, Jürgen; Schechter, Martin T; Strang, John; van den Brink, Wim

    2007-07-01

    Since the initial Swiss heroin-assisted treatment (HAT) study conducted in the mid-1990s, several other jurisdictions in Europe and North America have implemented HAT trials. All of these studies embrace the same goal-investigating the utility of medical heroin prescribing for problematic opioid users-yet are distinct in various key details. This paper briefly reviews (initiated or completed) studies and their main parameters, including primary research objectives, design, target populations, outcome measures, current status and-where available-key results. We conclude this overview with some final observations on a decade of intensive HAT research in the jurisdictions examined, including the suggestion that there is a mounting onus on the realm of politics to translate the-largely positive-data from completed HAT science into corresponding policy and programming in order to expand effective treatment options for the high-risk population of illicit opioid users.

  10. Bosonization and vertex operator of supersymmetry $u_q(\\hat{sl}(n|1))$ for level $k$

    CERN Document Server

    Kojima, Takeo

    2012-01-01

    We construct a bosonization of the quantum superalgebra $U_q(\\hat{sl}(N|1))$ for an arbitrary level $k$. We construct the screening that commutes with the quantum superalgebra for an arbitrary level $k \

  11. Application of Human-Autonomy Teaming (HAT) Patterns to Reduced Crew Operations (RCO)

    Science.gov (United States)

    Shively, R. Jay; Brandt, Summer L.; Lachter, Joel; Matessa, Mike; Sadler, Garrett; Battiste, Henri

    2016-01-01

    As part of the Air Force - NASA Bi-Annual Research Council Meeting, slides will be presented on recent Reduced Crew Operations (RCO) work. Unmanned aerial systems, robotics, advanced cockpits, and air traffic management are all examples of domains that are seeing dramatic increases in automation. While automation may take on some tasks previously performed by humans, humans will still be required, for the foreseeable future, to remain in the system. The collaboration with humans and these increasingly autonomous systems will begin to resemble cooperation between teammates, rather than simple task allocation. It is critical to understand this human-autonomy teaming (HAT) to optimize these systems in the future. One methodology to understand HAT is by identifying recurring patterns of HAT that have similar characteristics and solutions. A methodology for identifying HAT patterns to an advanced cockpit project is discussed.

  12. Application of Human-Autonomy Teaming (HAT) Patterns to Reduce Crew Operations (RCO)

    Science.gov (United States)

    Shively, R. Jay; Brandt, Summer L.; Lachter, Joel; Matessa, Mike; Sadler, Garrett; Battiste, Henri

    2016-01-01

    Unmanned aerial systems, robotics, advanced cockpits, and air traffic management are all examples of domains that are seeing dramatic increases in automation. While automation may take on some tasks previously performed by humans, humans will still be required, for the foreseeable future, to remain in the system. The collaboration with humans and these increasingly autonomous systems will begin to resemble cooperation between teammates, rather than simple task allocation. It is critical to understand this human-autonomy teaming (HAT) to optimize these systems in the future. One methodology to understand HAT is by identifying recurring patterns of HAT that have similar characteristics and solutions. This paper applies a methodology for identifying HAT patterns to an advanced cockpit project.

  13. Midterm evaluation of hemodynamics of the Top Hat supraannular aortic valve

    DEFF Research Database (Denmark)

    Aagaard, Jan; Nissen, Henrik; Geha, Alexander S

    2010-01-01

    The CarboMedics Top Hat supraannular aortic valve provides an orifice-to-annulus ratio of 1:1 in most patients, and enhances patient outcomes. This study compared the midterm echocardiographic parameters of 52 patients undergoing aortic valve replacement with a Top Hat valve with those in other...... measurements were compared in 38 patients with aortic valve stenosis. Hemodynamic data were comparable to those of other studies, but the Top Hat prosthesis implanted was significantly larger (by a mean of 3.29 mm) than the valve size indicated using an intraannular valve sizer in 48 patients. Mean effective...... orifice area improved significantly from 0.73 cm(2) preoperatively to 2.04 cm(2) postoperatively. In patients with preoperative aortic valve stenosis, postoperative mean peak gradient was significantly reduced. The larger orifice-to-annulus ratio of the Top Hat valve improved hemodynamic parameters...

  14. Parameters of Recent Transits of HAT-P-23b

    CERN Document Server

    Ramón-Fox, Felipe G

    2012-01-01

    Four transits of the exoplanet HAT-P-23b were recently observed with the 0.36m telescope at the Universidad de Monterrey Observatory. The four light curves were successfully combined to obtain a resulting one with reduced scattering per bin. This one was modeled using a Monte Carlo method to obtain the essential parameters that characterize the system. Assuming orbital parameters such as eccentricity, e, and longitude of periastron, w, from the discovery paper, we found values of Rp/R* = 0.1105 +0.0015-0.0013 for the planet-to-star radius ratio, a/R* = 4.23 +0.06-0.12 for the scaled semimajor axis, and an orbital inclination of the system of i = 87.9d +1.5-2.2. We also derive an improved orbital period of 1.2128868 +- 0.0000004 days (To = 2,454,852.26542 +- 0.00018 BJD_TDB) for the system.

  15. PARAMETERS OF RECENT TRANSITS OF HAT-P-23b

    Directory of Open Access Journals (Sweden)

    F. G. Ramón-Fox

    2013-01-01

    Full Text Available Cuatro tránsitos del planeta extrasolar HAT-P-23b fueron observados recientemente con el telescopio de 0.36 m del Observatorio de la Universidad de Monterrey. Las cuatro curvas de luz fueron exitosamente combinadas para obtener una resultante de mayor calidad. Esta curva fue modelada utilizando un método de Monte Carlo para obtener los parámetros esenciales que caracterizan al sistema. Asumiendo parámetros orbitales como la excentricidad e y la longitud del periastro ! reportados en el artículo del descubrimiento, encontramos valores de Rp/R* = 0.1105+0.0015 −0.0013 para la razón del radio del planeta al radio estelar, a/R? = 4.23+0.06 −0.12 para el semieje mayor normalizado, y una inclinación orbital de i = 87.9?+1.5 −2.2. Obtenemos un período orbital de 1.2128868 ± 0.0000004 días (To = 2, 454, 852.26542 ± 0.00018 BJD TDB.

  16. Structural Behavior of H+Hat Combined Steel Sheet Pile%H+Hat 组合型钢板桩结构性能研究

    Institute of Scientific and Technical Information of China (English)

    赵海丰; 永津亮祐; 项伟; 桂树强

    2015-01-01

    H+Hat 组合型钢板桩构造简单、抗弯刚度大、可反复使用且经济性好,具有广泛的应用前景,但在国内尚未应用而缺乏验证。通过理论计算、室内试验、现场原位试验和数值模拟等手段对H+Hat 组合型钢板桩的截面抗弯、锁口抗拉及锁口止水等结构性能进行了研究。研究结果显示:H+Hat 组合型钢板桩具有比普通钢板桩更大的抗弯截面模量,且其抗弯工作性能良好;锁口抗拉容许强度远大于其实际运行的抗拉强度;锁口止水性能不差于普通钢板桩。研究为H+Hat 组合型钢板桩在基坑、码头、船坞、围堰、库岸等工程中的推广应用提供了依据。%H+Hat combined steel sheet pile has a broad prospect of application due to its simple structure,big ben-ding stiffness,repeated usage and cost effectiveness.However,it has not been applied in China yet and lack of verification.In view of this we carried out theoretical calculation,indoor experiment,in situ test and numerical simulation to research the sectional flexural performance,lock tensile performance and lock waterproof performance of H+Hat combined steel sheet pile.Results reveal that the sectional flexural modulus of H+Hat combined steel sheet pile is larger than that of ordinary steel sheet pile with good flexural performance;the allowable lock tensile strength is far greater than that in actual operation;and the lock waterproof performance is not worse than that of or-dinary steel sheet pile.The research could be a basis for the popularization and application of H+Hat combined steel sheet pile in foundation pit,piers,docks,cofferdam,and bank supporting structures.

  17. HAT-P-42b and HAT-P-43b. Two inflated transiting hot Jupiters from the HATNet Survey

    Science.gov (United States)

    Boisse, I.; Hartman, J. D.; Bakos, G. Á.; Penev, K.; Csubry, Z.; Béky, B.; Latham, D. W.; Bieryla, A.; Torres, G.; Kovács, G.; Buchhave, L. A.; Hansen, T.; Everett, M.; Esquerdo, G. A.; Szklenár, T.; Falco, E.; Shporer, A.; Fulton, B. J.; Noyes, R. W.; Stefanik, R. P.; Lázár, J.; Papp, I.; Sári, P.

    2013-10-01

    Aims: We announce the discovery of two new transiting planets, and provide their accurate initial characterization. Methods: First identified from the HATNet wide-field photometric survey, these candidate transiting planets were then followed-up with a variety of photometric observations. Determining the planetary nature of the objects and characterizing the parameters of the systems were mainly done with the SOPHIE spectrograph at the 1.93 m telescope at OHP and the TRES spectrograph at the 1.5 m telescope at FLWO. Results: HAT-P-42b and HAT-P-43b are typical hot Jupiters on circular orbits around early-G/late-F main sequence host stars, with periods of 4.641878 ± 0.000032 and 3.332687 ± 0.000015 days, masses of 1.044 ± 0.083 and 0.662 ± 0.060 MJ, and radii of 1.280 ± 0.153 and 1.28+0.062-0.033RJ, respectively. These discoveries increase the sample of planets with measured mean densities, which are needed to constrain theories of planetary interiors and atmospheres. Moreover, their hosts are relatively bright (V < 13.5), which facilitates further follow-up studies. Full Table 2 is only available in electronic form at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/558/A86The photometric/spectroscopic data presented in this paper are based in part on observations carried out by the Hungarian-made Automated Telescope Network, using telescopes operated at the Fred Lawrence Whipple Observatory (FLWO) of the Smithsonian Astrophysical Observatory (SAO), and at the Submillimeter Array (SMA) of SAO, by the Tillinghast Reflector 1.5 m telescope and the 1.2 m telescope, both operated by SAO at FLWO, by the SOPHIE spectrograph mounted on the 1.93 m telescope at Observatoire de Haute Provence, France (runs DDT-Dec. 2011), by the Nordic Optical Telescope, operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway, and Sweden, in the Spanish Observatorio del Roque de los

  18. No isomorphism between the affine $\\hat sl(2)$ algebra and the N=2 superconformal algebras

    CERN Document Server

    Gato-Rivera, Beatriz

    2008-01-01

    Since 1999 it became obvious that the would be `isomorphism' between the affine $\\hat sl(2)$ algebra and the N=2 superconformal algebras, proposed by some authors, simply does not work. However, this issue was never properly discussed in the literature and, as a result, some confusion still remains. In this article we finally settle down, clearly and unambiguously, the true facts: there is no isomorphism between the affine $\\hat sl(2)$ algebra and the N=2 superconformal algebras.

  19. HATS-17b: A Transiting Compact Warm Jupiter in a 16.3 Days Circular Orbit

    CERN Document Server

    Brahm, R; Bakos, G Á; Penev, K; Espinoza, N; Rabus, M; Hartman, J D; Bayliss, D; Ciceri, S; Zhou, G; Mancini, L; Tan, T G; de Val-Borro, M; Bhatti, W; Csubry, Z; Bento, J; Henning, T; Schmidt, B; Suc, V; Lázár, J; Papp, I; Sári, P

    2015-01-01

    We report the discovery of HATS-17b, the first transiting warm Jupiter of the HATSouth network. HATS-17b transits its bright (V=12.4) G-type (M$_{\\star}$=1.131 $\\pm$ 0.030 M$_{\\odot}$, R$_{\\star}$=1.091$^{+0.070}_{-0.046}$ R$_{\\star}$) metal-rich ([Fe/H]=+0.3 dex) host star in a circular orbit with a period of P=16.2546 days. HATS-17b has a very compact radius of 0.777 $\\pm$ 0.056 R$_J$ given its Jupiter-like mass of 1.338 $\\pm$ 0.065 M$_J$. Up to 50% of the mass of HATS-17b may be composed of heavy elements in order to explain its high density with current models of planetary structure. HATS-17b is the longest period transiting planet discovered to date by a ground-based photometric survey, and is one of the brightest transiting warm Jupiter systems known. The brightness of HATS-17b will allow detailed follow-up observations to characterize the orbital geometry of the system and the atmosphere of the planet.

  20. HAT-P-18b and HAT-P-19b: Two Low-density Saturn-mass Planets Transiting Metal-rich K Stars

    Science.gov (United States)

    Hartman, J. D.; Bakos, G. Á.; Sato, B.; Torres, G.; Noyes, R. W.; Latham, D. W.; Kovács, G.; Fischer, D. A.; Howard, A. W.; Johnson, J. A.; Marcy, G. W.; Buchhave, L. A.; Füresz, G.; Perumpilly, G.; Béky, B.; Stefanik, R. P.; Sasselov, D. D.; Esquerdo, G. A.; Everett, M.; Csubry, Z.; Lázár, J.; Papp, I.; Sári, P.

    2011-01-01

    We report the discovery of two new transiting extrasolar planets. HAT-P-18b orbits the V = 12.759 K2 dwarf star GSC 2594-00646, with a period P = 5.508023 ± 0.000006 days, transit epoch Tc = 2454715.02174 ± 0.00020 (BJD), and transit duration 0.1131 ± 0.0009 days. The host star has a mass of 0.77 ± 0.03 M sun, radius of 0.75 ± 0.04 R sun, effective temperature 4803 ± 80 K, and metallicity [Fe/H] = +0.10 ± 0.08. The planetary companion has a mass of 0.197 ± 0.013 M J and radius of 0.995 ± 0.052 R J, yielding a mean density of 0.25 ± 0.04 g cm-3. HAT-P-19b orbits the V = 12.901 K1 dwarf star GSC 2283-00589, with a period P = 4.008778 ± 0.000006 days, transit epoch Tc = 2455091.53417 ± 0.00034 (BJD), and transit duration 0.1182 ± 0.0014 days. The host star has a mass of 0.84 ± 0.04 M sun, radius of 0.82 ± 0.05 R sun, effective temperature 4990 ± 130 K, and metallicity [Fe/H] = +0.23 ± 0.08. The planetary companion has a mass of 0.292 ± 0.018 M J and radius of 1.132 ± 0.072 R J, yielding a mean density of 0.25 ± 0.04 g cm-3. The radial velocity residuals for HAT-P-19 exhibit a linear trend in time, which indicates the presence of a third body in the system. Comparing these observations with theoretical models, we find that HAT-P-18b and HAT-P-19b are each consistent with a hydrogen-helium-dominated gas giant planet with negligible core mass. HAT-P-18b and HAT-P-19b join HAT-P-12b and WASP-21b in an emerging group of low-density Saturn-mass planets, with negligible inferred core masses. However, unlike HAT-P-12b and WASP-21b, both HAT-P-18b and HAT-P-19b orbit stars with super-solar metallicity. This calls into question the heretofore suggestive correlation between the inferred core mass and host star metallicity for Saturn-mass planets. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NOAO (A146Hr, A201Hr

  1. Derivation, Expansion, and Motor Neuron Differentiation of Human-Induced Pluripotent Stem Cells with Non-Integrating Episomal Vectors and a Defined Xenogeneic-free Culture System.

    Science.gov (United States)

    Hu, Wentao; He, Yongpei; Xiong, Yongjie; Lu, Hong; Chen, Hong; Hou, Limin; Qiu, Zhandong; Fang, Yu; Zhang, Suming

    2016-04-01

    Induced pluripotent stem cells (iPSCs) generated from patient-derived somatic cells provides the opportunity for model development in order to study patient-specific disease states with the potential for drug discovery. However, use of lentivirus and exposure of iPSCs to animal-derived products limit their therapeutic utility and affect lineage differentiation and subsequent downstream functionality of iPSC derivatives. Within the context of this study, we describe a simple and practical protocol enabling the efficient reprogramming of terminally differentiated adult fibroblasts into integration-free human iPSCs (hiPSCs) using a combination of episomal plasmids with small molecules (SMs). Using this approach, there was a 10-fold increase in reprogramming efficiency over single plasmid vector-based methods. We obtained approximately 100 iPSCs colonies from 1 × 10(5) human adult dermal fibroblasts (HADFs) and achieved approximately 0.1% reprogramming efficiencies. Concurrently, we developed a highly conducive culture system using xeno-free media and human vitronectin. The resulting hiPSCs were free of DNA integration and had completely lost episomal vectors, maintained long-term self-renewal, featured a normal karyotype, expressed pluripotent stem cell markers, and possessed the capability of differentiating into components of all three germ layers in vivo. Finally, we demonstrate that the integration-free hiPSCs could be differentiated into motor neurons under xeno-free culture conditions. This induction method will promote the derivation of patient-specific integration-free and xeno-free iPSCs and improve the strategy for motor neuron derivation. Our approach provides a useful tool for human disease models, drug screen, and clinical applications.

  2. Remedial Action Plan for the codisposal and stabilization of the Monument Valley and Mexican Hat uranium mill tailings at Mexican Hat, Utah: Appendices C--E. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-02-01

    This document provides appendices C, D, and E this Remedial Action Plan (RAP) which is a revision of the original Mexican Hat Remedial Action Plan and RAP Modification submitted in July 1988 and January 1989, respectively, along with updated design documents. Appendix C provide the Radiological Support Plan, Appendix D provides the Site Characterization, and Appendix E provides the Water Resources Protection Strategy.

  3. Top Hat HELLISH (Hot electron light emitting and lasing in heterostructures)

    CERN Document Server

    Wah, J Y

    2003-01-01

    Hot electron light emitting and lasing in semiconductor heterostructures (HELLISH) is a longitudinal transport structure comprising of a GaAs Quantum Well within an Al sub x Ga sub 1 sub - sub x As pn junction. The light emission from the HELLISH device was previously believed to be merely due to hot electron effects. In the current work, we showed, however, that the device can be operated even at low applied electric fields where the hot electron effects are essentially negligible. Thus, a novel model for operation with the new concept of 'quasi-flatband condition' is introduced. In order to investigate new functionalities of the HELLISH devices, a new structure with longer p-channel, nicknamed Top Hat HELLISH (THH), is also fabricated and demonstrated. In terms of the energy band profile, the HELLISH device has a monotonic build-in potential barrier along the heterojunction. The operation is through tilting the bands and hence allowing carrier diffusion into the active region under the quasi-flatband condit...

  4. Factorizing twists and R-matrices for representations of the quantum affine algebra U_q(\\hat sl_2)

    OpenAIRE

    Pfeiffer, Hendryk

    2000-01-01

    We calculate factorizing twists in evaluation representations of the quantum affine algebra U_q(\\hat sl_2). From the factorizing twists we derive a representation independent expression of the R-matrices of U_q(\\hat sl_2). Comparing with the corresponding quantities for the Yangian Y(sl_2), it is shown that the U_q(\\hat sl_2) results can be obtained by `replacing numbers by q-numbers'. Conversely, the limit q -> 1 exists in representations of U_q(\\hat sl_2) and both the factorizing twists and...

  5. HATS-8b: A Low-Density Transiting Super-Neptune

    CERN Document Server

    Bayliss, D; Bakos, G Á; Penev, K; Zhou, G; Brahm, R; Rabus, M; Jordán, A; Mancini, L; de Val-Borro, M; Bhatti, W; Espinoza, N; Csubry, Z; Howard, A W; Fulton, B J; Buchhave, L A; Henning, T; Schmidt, B; Ciceri, S; Noyes, R W; Isaacson, H; Marcy, G W; Suc, V; Lázár, J; Papp, I; Sári, P

    2015-01-01

    HATS-8b is a low density transiting super-Neptune discovered as part of the HATSouth project. The planet orbits its solar-like G dwarf host (V=14.03 $\\pm$ 0.10 and T$_{eff}$ =5679 $\\pm$ 50 K) with a period of 3.5839 d. HATS-8b is the third lowest mass transiting exoplanet to be discovered from a wide-field ground based search, and with a mass of 0.138 $\\pm$ 0.019 M$_J$ it is approximately half-way between the masses of Neptune and Saturn. However HATS-8b has a radius of 0.873 (+0.123,-0.075) R$_J$, resulting in a bulk density of just 0.259 $\\pm$ 0.091 g.cm$^{-3}$. The metallicity of the host star is super-Solar ([Fe/H]=0.210 $\\pm$ 0.080), arguing against the idea that low density exoplanets form from metal-poor environments. The low density and large radius of HATS-8b results in an atmospheric scale height of almost 1000 km, and in addition to this there is an excellent reference star of near equal magnitude at just 19 arcsecond separation on the sky. These factors make HATS-8b an exciting target for future a...

  6. HATS-5b: A Transiting hot-Saturn from the HATSouth Survey

    CERN Document Server

    Zhou, G; Penev, K; Bakos, G Á; Hartman, J D; Jordán, A; Mancini, L; Mohler, M; Csubry, Z; Ciceri, S; Brahm, R; Rabus, M; Buchhave, L; Henning, T; Suc, V; Espinoza, N; Béky, B; Noyes, R W; Schmidt, B; Butler, R P; Shectman, S; Thompson, I; Crane, J; Sato, B; Csák, B; Lázár, J; Papp, I; Sári, P; Nikolov, N

    2014-01-01

    We report the discovery of HATS-5b, a transiting hot-Saturn orbiting a G type star, by the HAT-South survey. HATS-5b has a mass of Mp=0.24 Mj, radius of Rp=0.91 Rj, and transits its host star with a period of P=4.7634d. The radius of HATS-5b is consistent with both theoretical and empirical models. The host star has a V band magnitude of 12.6, mass of 0.94 Msun, and radius of 0.87 Rsun. The relatively high scale height of HATS-5b, and the bright, photometrically quiet host star, make this planet a favourable target for future transmission spectroscopy follow-up observations. We reexamine the correlations in radius, equilibrium temperature, and metallicity of the close-in gas-giants, and find hot Jupiter-mass planets to exhibit the strongest dependence between radius and equilibrium temperature. We find no significant dependence in radius and metallicity for the close-in gas-giant population.

  7. An Elliptic Algebra $U_{q,p}(\\hat{sl_2})$ and the Fusion RSOS Model

    CERN Document Server

    Konno, H

    1997-01-01

    We introduce an elliptic algebra $U_{q,p}(\\hat{sl_2})$ with $p=q^{2r} (r\\in show that this algebra governs a structure of the space of states in the $k-$fusion RSOS model specified by a pair of positive integers $(r,k)$, or equivalently a $q-$deformation of the coset conformal field theory $SU(2)_k\\times SU(2)_{r-k-2}/SU(2)_{r-2}$. Extending the work by Lukyanov and Pugai corresponding to the case $k=1$, we gives a full set of screening operators for $k>1$. The algebra $U_{q,p}(\\hat{sl_2})$ has two interesting degeneration limits, $p\\to 0$ and $p\\to 1$. The former limit yields the quantum affine algebra $U_{q}(\\hat{sl_2})$ whereas the latter yields the algebra ${\\cal A}_{\\hbar,\\eta}(\\hat{sl_2})$, the scaling limit of the elliptic algebra ${\\cal A}_{q,p}(\\hat{sl_2})$. Using this correspondence, we also obtain the highest component of two types of vertex operators which can be regarded as $q-$deformations of the primary fields in the coset conformal field theory.

  8. Dynamical Constraints on the Core Mass of Hot Jupiter HAT-P-13b

    CERN Document Server

    Buhler, Peter; Batygin, Konstantin; Fulton, BJ; Fortney, Jonathan; Burrows, Adam; Wong, Ian

    2016-01-01

    HAT-P-13b is a Jupiter-mass transiting exoplanet that has settled onto a stable, short-period, and mildly eccentric orbit as a consequence of the action of tidal dissipation and perturbations from a second, highly eccentric, outer companion. Due to the special orbital configuration of the HAT-P-13 system, the magnitude of HAT-P-13b's eccentricity ($e_b$) is in part dictated by its Love number ($k_{2_b}$), which is in turn a proxy for the degree of central mass concentration in its interior. Thus, the measurement of $e_b$ constrains $k_{2_b}$ and allows us to place otherwise elusive constraints on the mass of HAT-P-13b's core ($M_{\\rm{core,b}}$). In this study we derive new constraints on the value of $e_b$ by observing two secondary eclipses of HAT-P-13b with the Infrared Array Camera on board the $\\textit{Spitzer Space Telescope}$. We fit the measured secondary eclipse times simultaneously with radial velocity measurements and find that $e_b = 0.00700 \\pm 0.00100$. We then use octupole-order secular perturba...

  9. HATS-1b: The First Transiting Planet Discovered by the HATSouth Survey

    CERN Document Server

    Penev, K; Bayliss, D; Jordán, A; Mohler, M; Zhou, G; Suc, V; Rabus, M; Hartman, J D; Mancini, L; Béky, B; Csubry, Z; Buchhave, L; Henning, T; Nikolov, N; Csák, B; Brahm, R; Espinoza, N; Conroy, P; Noyes, R W; Sasselov, D D; Schmidt, B; Wright, D J; Tinney, C G; Addison, B C; Lázár, J; Papp, I; Sári, P

    2012-01-01

    We report the discovery of HATS-1b, a transiting extrasolar planet orbiting the moderately bright V=12.05 G dwarf star GSC 6652-00186, and the first planet discovered by HATSouth, a global network of autonomous wide-field telescopes. HATS-1b has a period P~3.4465 d, mass Mp~1.86MJ, and radius Rp~1.30RJ. The host star has a mass of 0.99Msun, and radius of 1.04Rsun. The discovery light curve of HATS-1b has near continuous coverage over several multi-day periods, demonstrating the power of using a global network of telescopes to discover transiting planets.

  10. Infinitely many commuting operators for the elliptic quantum group $U_{q,p}(\\hat{sl_N})$

    CERN Document Server

    Kojima, Takeo

    2011-01-01

    We construct two classes of infinitely many commuting operators associated with the elliptic quantum group $U_{q,p}(\\hat{sl_N})$. We call one of them the integral of motion ${\\cal G}_m$, $(m \\in {\\mathbb N})$ and the other the boundary transfer matrix $T_B(z)$, $(z \\in {\\mathbb C})$. The integral of motion ${\\cal G}_m$ is related to elliptic deformation of the $N$-th KdV theory. The boundary transfer matrix $T_B(z)$ is related to the boundary $U_{q,p}(\\hat{sl_N})$ face model. We diagonalize the boundary transfer matrix $T_B(z)$ by using the free field realization of the elliptic quantum group, however diagonalization of the integral of motion ${\\cal G}_m$ is open problem even for the simplest case $U_{q,p}(\\hat{sl_2})$.

  11. [Usefulness of top-hat transform processing in whole body bone scintigraphy].

    Science.gov (United States)

    Kita, Akinobu; Sugimoto, Katsuya; Tsuchida, Tatsurou; Kishimoto, Takahiro; Toi, Akiko; Shimada, Masato; Adachi, Toshiki

    2013-01-01

    To assess the usefulness of top-hat transform processing in whole body bone scintigraphy, five radiological technicians interpreted both original and top-hat processed images to determine the improvement of lesion detectability and interpretation time. For the evaluation of detectability, receiver operating characteristic (ROC) analysis was performed. The area under the curve (AUC) calculated from the ROC curve was improved in all observers (from 0.786 to 0.864 in average), although no significant difference was observed. However, the interpretation time was improved significantly (from 24.5 to 16.2 s in average). Top-hat transform processing in whole body bone scintigraphy is thought to be useful for the improvement of lesion detectability and interpretation time.

  12. HATS-1b: THE FIRST TRANSITING PLANET DISCOVERED BY THE HATSouth SURVEY

    Energy Technology Data Exchange (ETDEWEB)

    Penev, K.; Bakos, G. A.; Hartman, J. D.; Csubry, Z. [Department of Astrophysical Sciences, Princeton University, NJ 08544 (United States); Bayliss, D.; Zhou, G.; Conroy, P. [Australian National University, Canberra (Australia); Jordan, A.; Suc, V.; Rabus, M.; Brahm, R.; Espinoza, N. [Departamento de Astronomia y Astrofisica, Pontificia Universidad Catolica de Chile, Av. Vicuna Mackenna 4860, 7820436 Macul, Santiago (Chile); Mohler, M.; Mancini, L.; Henning, T.; Nikolov, N.; Csak, B. [Max Planck Institute for Astronomy, Heidelberg (Germany); Beky, B.; Noyes, R. W. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA (United States); Buchhave, L., E-mail: kpenev@astro.princeton.edu [Niels Bohr Institute, Copenhagen University (Denmark); and others

    2013-01-01

    We report the discovery of HATS-1b, a transiting extrasolar planet orbiting the moderately bright V = 12.05 G dwarf star GSC 6652-00186, and the first planet discovered by HATSouth, a global network of autonomous wide-field telescopes. HATS-1b has a period of P Almost-Equal-To 3.4465 days, mass of M{sub p} Almost-Equal-To 1.86 M{sub J}, and radius of R{sub p} Almost-Equal-To 1.30 R{sub J}. The host star has a mass of 0.99 M{sub Sun} and radius of 1.04 R{sub Sun }. The discovery light curve of HATS-1b has near-continuous coverage over several multi-day timespans, demonstrating the power of using a global network of telescopes to discover transiting planets.

  13. Flexural fatigue life prediction of closed hat-section using materially nonlinear axial fatigue characteristics

    Science.gov (United States)

    Razzaq, Zia

    1989-01-01

    Straight or curved hat-section members are often used as structural stiffeners in aircraft. For instance, they are employed as stiffeners for the dorsal skin as well as in the aerial refueling adjacent area structure in F-106 aircraft. The flanges of the hat-section are connected to the aircraft skin. Thus, the portion of the skin closing the hat-section interacts with the section itself when resisting the stresses due to service loads. The flexural fatigue life of such a closed section is estimated using materially nonlinear axial fatigue characteristics. It should be recognized that when a structural shape is subjected to bending, the fatigue life at the neutral axis is infinity since the normal stresses are zero at that location. Conversely, the fatigue life at the extreme fibers where the normal bending stresses are maximum can be expected to be finite. Thus, different fatigue life estimates can be visualized at various distances from the neural axis. The problem becomes compounded further when significant portions away from the neutral axis are stressed into plastic range. A theoretical analysis of the closed hat-section subjected to flexural cyclic loading is first conducted. The axial fatigue characteristics together with the related axial fatigue life formula and its inverted form given by Manson and Muralidharan are adopted for an aluminum alloy used in aircraft construction. A closed-form expression for predicting the flexural fatigue life is then derived for the closed hat-section including materially nonlinear action. A computer program is written to conduct a study of the variables such as the thicknesses of the hat-section and the skin, and the type of alloy used. The study has provided a fundamental understanding of the flexural fatigue life characteristics of a practical structural component used in aircraft when materially nonlinear action is present.

  14. Mercury in Connecticut and Long Island Sound: Impact of Historic Hatting Industries

    Science.gov (United States)

    Bronsther, R.; Welsh, P.; Varekamp, J. C.

    2004-05-01

    Wetlands in the northeastern region of the U.S.A. are mildly contaminated with Hg as a result of atmospheric deposition, with modern soil concentrations of several 100 ppb Hg. Connecticut was once considered the hat manufacturing capital of the world. A solution of Hg-nitrate was used in the felting process, and old hat factory sites have become point sources of Hg contamination. Surface soils in the former hatting town of Danbury have Hg soil concentration levels that well exceed Connecticut's residential soil remediation standard of 20 ppm. Sediments from the Still River, a small waterway that runs through Danbury and discharges into the Housatonic River, show locally Hg concentrations of several tens of ppm, Cores taken from marsh islands in the Housatonic River show elevated Hg concentrations as well, up to 5 ppm Hg. Sites in Norwalk, another former hatting town, and along the Norwalk River also show values of more than 5 ppm Hg. The old hat factory sites in both towns clearly serve as point sources for Hg contamination downstream. Cores taken from marshes in the Connecticut River, which drains no former hatting towns, had much lower Hg concentrations (up to ~500 ppb Hg). The Five Mile River marsh near Darien, CT has lower peak values than found in the sediments of the Housatonic and Norwalk River cores, but still slightly elevated (800 ppb Hg). The Hg from the hat-site point sources is ultimately entering Long Island Sound. High Hg levels are found in western Long Island Sound compared to the eastern section (up to 800 ppb Hg), which is the result of fine-grained sediment transport westwards in the Sound, and the release of Hg-bearing effluent from waste water treatment plants of New York City. The contaminated sediment output from the Housatonic and Norwalk Rivers also contributes to the elevated Hg levels in the western Sound and possibly the Five Mile River marshes. Cores taken from the Housatonic River and western Long Island Sound show also peak Hg

  15. Red Hat Enterprise Virtualization - KVM-based infrastructure services at BNL

    Energy Technology Data Exchange (ETDEWEB)

    Cortijo, D.

    2011-06-14

    Over the past 18 months, BNL has moved a large percentage of its Linux-based servers and services into a Red Hat Enterprise Virtualization (RHEV) environment. This presentation will address our approach to virtualization, critical decision points, and a discussion of our implementation. Specific topics will include an overview of hardware and software requirements, networking, and storage; discussion of the decision of Red Hat solution over competing products (VMWare, Xen, etc); details on some of the features of RHEV - both current and on their roadmap; Review of performance and reliability gains since deployment completion; path forward for RHEV at BNL and caveats and potential problems.

  16. [Remedial action plan for the codisposal and stabilization of the Monument Valley and Mexican Hat uranium mill tailings at Mexican Hat, Utah]. Appendix F, Groundwater hydrology calculations

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-12-31

    This document contains the ground water hydrology calculations for the remedial action plan for the codisposal and stabilization of uranium mill tailings at Mexican Hat, Utah. Included are calculations for the following: slug test analyses for monitor wells, analyses of packer tests, hydraulic gradients and ground water velocities, volume of released water, aquifer pumping test analysis, slug test analysis to determine hydraulic conductivity, and gradient calculations.

  17. HAT-P-34b-HAT-P-37b: Four Transiting Planets More Massive than Jupiter Orbiting Moderately Bright Stars

    Science.gov (United States)

    Bakos, G. Á.; Hartman, J. D.; Torres, G.; Béky, B.; Latham, D. W.; Buchhave, L. A.; Csubry, Z.; Kovács, G.; Bieryla, A.; Quinn, S.; Szklenár, T.; Esquerdo, G. A.; Shporer, A.; Noyes, R. W.; Fischer, D. A.; Johnson, J. A.; Howard, A. W.; Marcy, G. W.; Sato, B.; Penev, K.; Everett, M.; Sasselov, D. D.; Fűrész, G.; Stefanik, R. P.; Lázár, J.; Papp, I.; Sári, P.

    2012-07-01

    We report the discovery of four transiting extrasolar planets (HAT-P-34b-HAT-P-37b) with masses ranging from 1.05 to 3.33 M J and periods from 1.33 to 5.45 days. These planets orbit relatively bright F and G dwarf stars (from V = 10.16 to V = 13.2). Of particular interest is HAT-P-34b which is moderately massive (3.33 M J), has a high eccentricity of e = 0.441 ± 0.032 at a period of P = 5.452654 ± 0.000016 days, and shows hints of an outer component. The other three planets have properties that are typical of hot Jupiters. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NOAO (A289Hr) and NASA (N167Hr and N029Hr). Based in part on data collected at the Subaru Telescope, which is operated by the National Astronomical Observatory of Japan. Based in part on observations made with the Nordic Optical Telescope, operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway, and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias.

  18. Characterization of the atmosphere of the hot Jupiter HAT-P-32Ab and the M-dwarf companion HAT-P-32B

    CERN Document Server

    Zhao, Ming; Wright, Jason T; Knutson, Heather A; Burrows, Adam; Fortney, Johnathan; Ngo, Henry; Fulton, Benjamin J; Baranec, Christoph; Riddle, Reed; Law, Nicholas M; Muirhead, Philip S; Hinkley, Sasha; Showman, Adam P; Curtis, Jason; Burruss, Rick

    2014-01-01

    We report secondary eclipse photometry of the hot Jupiter HAT-P-32Ab, taken with Hale/WIRC in H and Ks bands and with Spitzer/IRAC at 3.6 and 4.5 micron. We carried out adaptive optics imaging of the planet host star HAT-P-32A and its companion HAT-P-32B in the near-IR and the visible. We clearly resolve the two stars from each other and find a separation of 2.923" +/- 0. 004" and a position angle 110.64 deg +/- 0.12 deg. We measure the flux ratios of the binary in g' r' i' z' and H & Ks bands, and determine Teff = 3565 +/- 82 K for the companion star, corresponding to an M1.5 dwarf. We use PHOENIX stellar atmosphere models to correct the dilution of the secondary eclipse depths of the hot Jupiter due to the presence of the M1.5 companion. We also improve the secondary eclipse photometry by accounting for the non-classical, flux-dependent nonlinearity of the WIRC IR detector in the H band. We measure planet-to-star flux ratios of 0.090 +/- 0.033%, 0.178 +/- 0.057%, 0.364 +/- 0.016%, and 0.438 +/- 0.020% i...

  19. Boundary state of $U_q(\\hat{gl}(N|N))$ analog of half-infinite $t-J$ model

    CERN Document Server

    Kojima, Takeo

    2015-01-01

    The $U_q(\\hat{gl}(N|N))$-analog of the half-infinite $t-J$ model with a boundary is considered by using the vertex operator approach. We find explicit bosonic formula of the boundary state in the integrable highest-weight module over the quantum superalgebra $U_q(\\hat{gl}(N|N))$.

  20. WASP-40b: independent discovery of the 0.6-Mjup transiting exoplanet HAT-P-27b

    CERN Document Server

    Anderson, D R; Boisse, I; Bouchy, F; Collier-Cameron, A; Faedi, F; Hebrard, G; Hellier, C; Lendl, M; Moutou, C; Pollacco, D; Santerne, A; Smalley, B; Smith, A M S; Todd, I; Triaud, A H M J; West, R G; Wheatley, P J; Bento, J; Enoch, B; Gillon, M; Maxted, P F L; McCormac, J; Queloz, D; Simpson, E K; Skillen, I

    2011-01-01

    From WASP photometry and SOPHIE radial velocities we report the discovery of WASP-40b (= HAT-P-27b), a 0.6-Mjup planet that transits its 12th magnitude host star every 3.04 d. The host star is of late-G or early-K type and likely has an above-Solar metallicity, with [Fe/H] = 0.14 +/- 0.11. The planet's mass and radius are typical of the known hot Jupiters, thus adding another system to the apparent pileup of transiting planets with periods near 3 to 4 d. Our parameters match those of the recent HATnet announcement of the same planet, thus giving confidence in the techniques used. We report a possible indication of stellar activity in the host star.

  1. Isolation and characterization of Vibrio cholerae isolates from seafood in Hat Yai City, Songkhla, Thailand.

    Science.gov (United States)

    Preeprem, Sutima; Mittraparp-arthorn, Pimonsri; Bhoopong, Phuangthip; Vuddhakul, Varaporn

    2014-11-01

    Seafood has been identified as an important source of Vibrio cholerae in Thailand, especially in the Southern coastal region. In this study, we isolated and characterized V. cholerae from seafood obtained from several markets in Hat Yai city, Southern Thailand. A total of 100 V. cholerae isolates were obtained from 55 of 125 seafood samples. The dominant serotype was non-O1/non-O139. Polymerase chain reaction (PCR) analysis was used to detect the presence of pathogenesis-related genes. The stn/sto and hlyA El Tor virulence genes were detected in 20% and 96% of the isolates, respectively. None of the isolates were positive for the ctxA, tcpA, zot, and ace genes. Only 6% of the isolates carried the T3SS gene (vcsV2); however, the majority of the isolates (96%) carried the T6SS gene (vasH). Representative isolates (n=35) that exhibited various virulence gene patterns were randomly selected and analyzed for their hemolytic activity, antibiotic susceptibility, biofilm formation, and genotype. Hemolytic activity using sheep red blood cells was detected in only one of the hlyA-negative isolates. Apart from ampicillin, all isolates were pansusceptible to five test antibiotics. Biofilm production was observed in most of the isolates, and there was no difference in the presence of a biofilm between the smooth and rugose isolates. Using the enterobacterial repetitive intergenic consensus-PCR method, clonal relationships were observed among the isolates that exhibited identical virulence gene patterns.

  2. Warm Spitzer and Palomar near-IR secondary eclipse photometry of two hot Jupiters: WASP-48b and HAT-P-23b

    Energy Technology Data Exchange (ETDEWEB)

    O' Rourke, Joseph G.; Knutson, Heather A.; Désert, Jean-Michel [Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125 (United States); Zhao, Ming [Department of Astronomy and Astrophysics, 525 Davey Laboratory, The Pennsylvania State University, University Park, PA 16802 (United States); Fortney, Jonathan J. [Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States); Burrows, Adam [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 05844 (United States); Agol, Eric [Department of Astronomy, University of Washington, Box 351580, Seattle, WA 98195 (United States); Deming, Drake [Department of Astronomy, University of Maryland, College Park, MD 20742 (United States); Howard, Andrew W. [Institute for Astronomy, University of Hawaii, 2680 Woodlawn Drive, Honolulu, HI 96822 (United States); Lewis, Nikole K. [Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Showman, Adam P. [Lunar and Planetary Laboratory, University of Arizona, Tucson, AZ 85721 (United States); Todorov, Kamen O. [Institute for Astronomy, ETH Zürich, Wolfgang-Pauli-Strasse 27, 8093 Zürich (Switzerland)

    2014-02-01

    We report secondary eclipse photometry of two hot Jupiters, WASP-48b and HAT-P-23b, at 3.6 and 4.5 μm taken with the InfraRed Array Camera aboard the Spitzer Space Telescope during the warm Spitzer mission and in the H and K{sub S} bands with the Wide Field IR Camera at the Palomar 200 inch Hale Telescope. WASP-48b and HAT-P-23b are Jupiter-mass and twice Jupiter-mass objects orbiting an old, slightly evolved F star and an early G dwarf star, respectively. In the H, K{sub S} , 3.6 μm, and 4.5 μm bands, respectively, we measure secondary eclipse depths of 0.047% ± 0.016%, 0.109% ± 0.027%, 0.176% ± 0.013%, and 0.214% ± 0.020% for WASP-48b. In the K{sub S} , 3.6 μm, and 4.5 μm bands, respectively, we measure secondary eclipse depths of 0.234% ± 0.046%, 0.248% ± 0.019%, and 0.309% ± 0.026% for HAT-P-23b. For WASP-48b and HAT-P-23b, respectively, we measure delays of 2.6 ± 3.9 minutes and 4.0 ± 2.4 minutes relative to the predicted times of secondary eclipse for circular orbits, placing 2σ upper limits on |ecos ω| of 0.0053 and 0.0080, both of which are consistent with circular orbits. The dayside emission spectra of these planets are well-described by blackbodies with effective temperatures of 2158 ± 100 K (WASP-48b) and 2154 ± 90 K (HAT-P-23b), corresponding to moderate recirculation in the zero albedo case. Our measured eclipse depths are also consistent with one-dimensional radiative transfer models featuring varying degrees of recirculation and weak thermal inversions or no inversions at all. We discuss how the absence of strong temperature inversions on these planets may be related to the activity levels and metallicities of their host stars.

  3. Nonlinear $\\hat{W}_{\\infty}$ Current Algebra in the SL(2,R)/U(1) Coset Model

    CERN Document Server

    Yu, F; Yu, Feng; Wu, Yong-Shi

    1992-01-01

    Previously we have established that the second Hamiltonian structure of the KP hierarchy is a nonlinear deformation, called $\\hat{W}_{\\infty}$, of the linear, centerless $W_{\\infty}$ algebra. In this letter we present a free-field realization for all generators of $\\hat{W}_{\\infty}$ in terms of two scalars as well as an elegant generating function for the $\\hat{W}_{\\infty}$ currents in the classical conformal $SL(2,R)/U(1)$ coset model. After quantization, a quantum deformation of $\\hat{W}_{\\infty}$ appears as the hidden current algebra in this model. The $\\hat{W}_{\\infty}$ current algebra results in an infinite set of commuting conserved charges, which might give rise to $W$-hair for the 2d black hole arising in the corresponding string theory at level $k=9/4$.

  4. Safe engineering of CAR T cells for adoptive cell therapy of cancer using long-term episomal gene transfer.

    Science.gov (United States)

    Jin, Chuan; Fotaki, Grammatiki; Ramachandran, Mohanraj; Nilsson, Berith; Essand, Magnus; Yu, Di

    2016-07-01

    Chimeric antigen receptor (CAR) T-cell therapy is a new successful treatment for refractory B-cell leukemia. Successful therapeutic outcome depends on long-term expression of CAR transgene in T cells, which is achieved by delivering transgene using integrating gamma retrovirus (RV) or lentivirus (LV). However, uncontrolled RV/LV integration in host cell genomes has the potential risk of causing insertional mutagenesis. Herein, we describe a novel episomal long-term cell engineering method using non-integrating lentiviral (NILV) vector containing a scaffold/matrix attachment region (S/MAR) element, for either expression of transgenes or silencing of target genes. The insertional events of this vector into the genome of host cells are below detection level. CD19 CAR T cells engineered with a NILV-S/MAR vector have similar levels of CAR expression as T cells engineered with an integrating LV vector, even after numerous rounds of cell division. NILV-S/MAR-engineered CD19 CAR T cells exhibited similar cytotoxic capacity upon CD19(+) target cell recognition as LV-engineered T cells and are as effective in controlling tumor growth in vivo We propose that NILV-S/MAR vectors are superior to current options as they enable long-term transgene expression without the risk of insertional mutagenesis and genotoxicity. © 2016 The Authors. Published under the terms of the CC BY 4.0 license.

  5. Low-copy episomal vector pFY20 and high-saturation coverage genomic libraries for the fission yeast Schizosaccharomyces pombe.

    Science.gov (United States)

    Wahls, Wayne P; Davidson, Mari K

    2008-09-01

    In fission yeast, as in many organisms, episomally replicating plasmid DNA molecules can be used for a wide variety of applications. However, replicating plasmids described previously are each propagated at a high copy number per cell. Plasmid fission yeast twenty (pFY20) contains the ura4(+) gene for positive and negative selection, an origin of replication (ars1) and a stability element (stb). Although this plasmid does not have a centromere, it is propagated with a copy number of about two plasmids per haploid genome equivalent and it is transmitted with relatively high fidelity in mitosis and meiosis. This low-copy vector is useful for screens and mutational studies where overexpression (e.g. from high copy plasmids) is undesirable. We therefore constructed multiple partial-digest, size-fractionated, fission yeast genomic DNA libraries in pFY20 and in the cloning vector pBluescript KS(+). These libraries have sufficient complexity (average of 2100 genome equivalents each) for saturation screening by complementation, plasmid shuffle or hybridization.

  6. Modification to the Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Mexican Hat, Utah: Volume 1, Text, Attachments 1--6. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1989-01-01

    This document provides the modifications to the 1988 Remedial Action Plan (RAP) of the contaminated materials at the Monument Valley, Arizona, and Mexican Hat, Utah. The text detailing the modifications and attachments 1 through 6 are provided with this document. The RAP was developed to serve a two-fold purpose. It presents the activities proposed by the Department of Energy (DOE) to accomplish long-term stabilization and control of the residual radioactive materials (RRM) from Monument Valley, Arizona, and Mexican Hat, Utah, at the Mexican Hat disposal site. It also serves to document the concurrence of both the Navajo Nation and the Nuclear Regulatory Commission (NRC) in the remedial action. This agreement, upon execution by DOE and the Navajo Nation and concurrence by the NRC, becomes Appendix B of the Cooperative Agreement. This document has been structured to provide a comprehensive understanding of the remedial action proposed for the Monument Valley and Mexican Hat sites. It includes specific design and construction requirements for the remedial action. Pertinent information and data are included with reference given to the supporting documents.

  7. WASP-12b and HAT-P-8b are Members of Triple Star Systems

    Science.gov (United States)

    Bechter, Eric B.; Crepp, Justin R.; Ngo, Henry; Knutson, Heather A.; Batygin, Konstantin; Hinkley, Sasha; Muirhead, Philip S.; Johnson, John Asher; Howard, Andrew W.; Montet, Benjamin T.; Matthews, Christopher T.; Morton, Timothy D.

    2014-06-01

    We present high spatial resolution images that demonstrate that WASP-12b and HAT-P-8b orbit the primary stars of hierarchical triple star systems. In each case, two distant companions with colors and brightnesses consistent with M dwarfs co-orbit the hot Jupiter planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. and Crossfield et al. into two distinct sources separated by 84.3 ± 0.6 mas (21 ± 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al., is in fact composed of two stars separated by 65.3 ± 0.5 mas (15 ± 1 AU). Our follow-up observations demonstrate physical association through common proper motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18 ± 0.02 M ⊙ using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well studied planet hosts now represent higher-order multi-star systems with potentially complex dynamics, underscoring the importance of diffraction-limited imaging and providing additional context for understanding the migrant population of transiting hot Jupiters.

  8. The Carnegie H.A.T. Project--History and Technology.

    Science.gov (United States)

    Laskey, Sue

    1991-01-01

    Describes the History and Technology Project (HAT) at a model technology school. Offers suggestions of suitable hardware and software, and ideas for funding. Emphasizes the goal of integrating technology into all levels of history and social science. Calls for increasing teacher-student interaction and restructuring teaching methods to reach all…

  9. Comparison of Protein Acetyltransferase Action of CRTAase with the Prototypes of HAT

    Directory of Open Access Journals (Sweden)

    Prija Ponnan

    2014-01-01

    Full Text Available Our laboratory is credited for the discovery of enzymatic acetylation of protein, a phenomenon unknown till we identified an enzyme termed acetoxy drug: protein transacetylase (TAase, catalyzing the transfer of acetyl group from polyphenolic acetates to receptor proteins (RP. Later, TAase was identified as calreticulin (CR, an endoplasmic reticulum luminal protein. CR was termed calreticulin transacetylase (CRTAase. Our persistent study revealed that CR like other families of histone acetyltransferases (HATs such as p300, Rtt109, PCAF, and ESA1, undergoes autoacetylation. The autoacetylated CR was characterized as a stable intermediate in CRTAase catalyzed protein acetylation, and similar was the case with ESA1. The autoacetylation of CR like that of HATs was found to enhance protein-protein interaction. CR like HAT-1, CBP, and p300 mediated the acylation of RP utilizing acetyl CoA and propionyl CoA as the substrates. The similarities between CRTAase and HATs in mediating protein acylation are highlighted in this review.

  10. Towards top-hat spatial shaping of ultrafast laser beam based on Zernike polynomials

    Science.gov (United States)

    Mauclair, Cyril; Faure, Nicolas; Houzet, Julien

    2016-04-01

    Femtosecond laser micro machining of surfaces knows a gain of interest as it demonstrates efficient and precise processing with reduced side effects around the irradiated zone, and also because of the remarkable costs reduction and reliability improvements of nowadays commercially available sources. Controlling the intensity distribution spatially can offer a supplementary degree of flexibility and precision in achieving user-defined ablation spatial profile, drilling, cutting of materials or in-volume laser-induced modifications. In this scope, the possibility to generate a top-hat intensity distribution by spatially shaping the beam wavefront is studied in this work. An optimization of Zernike polynomials coefficients is conducted to numerically determine an adequate phase mask that shapes the laser intensity distribution following a targeted top hat distribution in the processing plane, usually at the focal length of a converging lens. The efficiency of the method is numerically investigated in the optimization by evaluation of the root mean square error (RMS) between the top-hat target and the calculated laser distribution in the far field. We numerically verify that acceptable top-hat beam shaping of various size can be achieved with a sufficient number of Zernike polynomials, opening the way to phase mask calculations adapted to the wavefront modulator ability to reproduce Zernike polynomials.

  11. HATS-5b: A TRANSITING HOT SATURN FROM THE HATSouth SURVEY

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, G.; Bayliss, D.; Schmidt, B. [Research School of Astronomy and Astrophysics, Australian National University, Canberra, ACT 2611 (Australia); Penev, K.; Bakos, G. Á.; Hartman, J. D.; Csubry, Z. [Department of Astrophysical Sciences, Princeton University, NJ 08544 (United States); Jordán, A.; Brahm, R.; Rabus, M.; Suc, V.; Espinoza, N. [Departamento de Astronomía y Astrofísica, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, 7820436 Macul, Santiago (Chile); Mancini, L.; Mohler, M.; Ciceri, S.; Henning, T. [Max Planck Institute for Astronomy, Heidelberg (Germany); Buchhave, L. [Niels Bohr Institute, Copenhagen University (Denmark); Béky, B.; Noyes, R. W. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Butler, R. P., E-mail: george.zhou@anu.edu.au [Department of Terrestrial Magnetism, Carnegie Institution of Washington, 5241 Broad Branch Road NW, Washington, DC 20015-1305 (United States); and others

    2014-06-01

    We report the discovery of HATS-5b, a transiting hot Saturn orbiting a G-type star, by the HATSouth survey. HATS-5b has a mass of M{sub p} ≈ 0.24 M {sub J}, radius of R{sub p} ≈ 0.91 R {sub J}, and transits its host star with a period of P ≈ 4.7634 days. The radius of HATS-5b is consistent with both theoretical and empirical models. The host star has a V-band magnitude of 12.6, mass of 0.94 M {sub ☉}, and radius of 0.87 R {sub ☉}. The relatively high scale height of HATS-5b and the bright, photometrically quiet host star make this planet a favorable target for future transmission spectroscopy follow-up observations. We reexamine the correlations in radius, equilibrium temperature, and metallicity of the close-in gas giants and find hot Jupiter-mass planets to exhibit the strongest dependence between radius and equilibrium temperature. We find no significant dependence in radius and metallicity for the close-in gas giant population.

  12. WASP-12b and HAT-P-8b are Members of Triple Star Systems

    CERN Document Server

    Bechter, Eric B; Ngo, Henry; Knutson, Heather A; Batygin, Konstantin; Hinkley, Sasha; Muirhead, Phillip S; Johnson, John Asher; Howard, Andrew W; Montet, Benjamin T; Matthews, Christopher T; Morton, Timothy D

    2013-01-01

    We present high spatial resolution images that demonstrate the hot Jupiters WASP-12b and HAT-P-8b orbit the primary star of hierarchical triple star systems. In each case, two distant companions with colors and brightness consistent with M dwarfs co-orbit the planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. 2011 and Crossfield et al. 2012, into two distinct sources separated by 84.3+/-0.6 mas (21 +/- 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al. 2011, is in fact composed of two stars separated by 65.3+/-0.5 mas (15+/-1 AU). Our follow-up observations demonstrate physical association through common proper-motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18+/-0.02Msun using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well-studied pl...

  13. Maximizing prosthetic valve size with the Top Hat supra-annular aortic valve

    DEFF Research Database (Denmark)

    Aagaard, Jan; Geha, Alexander S.

    2007-01-01

    BACKGROUND AND AIM OF THE STUDY: The CarboMedics Top Hat supra-annular aortic valve allows a one-size (and often two-size) increase over the standard intra-annular valve. This advantage should minimize the risk of patient-prosthesis mismatch, where the effective prosthetic valve orifice area...

  14. The Mexican hat effect on the delamination buckling of a compressed thin film

    Science.gov (United States)

    Zhang, Yin; Liu, Yun

    2014-12-01

    Because of the interaction between film and substrate, the film buckling stress can vary significantly, depending on the delamination geometry, the film and substrate mechanical properties. The Mexican hat effect indicates such interaction. An analytical method is presented, and related dimensional analysis shows that a single dimensionless parameter can effectively evaluate the effect.

  15. Planet-induced Stellar Pulsations in HAT-P-2's Eccentric System

    Science.gov (United States)

    de Wit, Julien; Lewis, Nikole K.; Knutson, Heather A.; Fuller, Jim; Antoci, Victoria; Fulton, Benjamin J.; Laughlin, Gregory; Deming, Drake; Shporer, Avi; Batygin, Konstantin; Cowan, Nicolas B.; Agol, Eric; Burrows, Adam S.; Fortney, Jonathan J.; Langton, Jonathan; Showman, Adam P.

    2017-02-01

    Extrasolar planets on eccentric short-period orbits provide a laboratory in which to study radiative and tidal interactions between a planet and its host star under extreme forcing conditions. Studying such systems probes how the planet’s atmosphere redistributes the time-varying heat flux from its host and how the host star responds to transient tidal distortion. Here, we report the insights into the planet–star interactions in HAT-P-2's eccentric planetary system gained from the analysis of ∼350 hr of 4.5 μm observations with the Spitzer Space Telescope. The observations show no sign of orbit-to-orbit variability nor of orbital evolution of the eccentric planetary companion, HAT-P-2 b. The extensive coverage allows us to better differentiate instrumental systematics from the transient heating of HAT-P-2 b’s 4.5 μm photosphere and yields the detection of stellar pulsations with an amplitude of approximately 40 ppm. These pulsation modes correspond to exact harmonics of the planet’s orbital frequency, indicative of a tidal origin. Transient tidal effects can excite pulsation modes in the envelope of a star, but, to date, such pulsations had only been detected in highly eccentric stellar binaries. Current stellar models are unable to reproduce HAT-P-2's pulsations, suggesting that our understanding of the interactions at play in this system is incomplete.

  16. The TopHat experiment: A balloon-borne instrument for mapping millimeter and submillimeter emission

    DEFF Research Database (Denmark)

    Silverberg, R.F.; Cheng, E.S.; Aguirre, J.E.

    2005-01-01

    The TopHat experiment was designed to measure the anisotropy in the cosmic microwave background radiation on angular scales from 0.degrees 3 to 30 degrees and the thermal emission from both Galactic and extragalactic dust. The balloon-borne instrument had five spectral bands spanning frequencies...

  17. Clinical study on HAT and SEDAN score scales and related risk factors for predicting hemorrhagic transformation following thrombolysis in acute ischemic stroke

    Directory of Open Access Journals (Sweden)

    Heng WEI

    2015-03-01

    Full Text Available Objective To investigate the value of HAT and SEDAN score scales in predicting hemorrhagic transformation (HT following the recombinant tissue-type plasminogen activator (rt-PA intravenous thrombolysis in acute ischemic stroke patients and risk factors affecting HT.  Methods A total of 143 patients with acute ischemic stroke underwent rt-PA intravenous thrombolysis within 4.50 h of onset and their clinical data were collected. According to head CT after thrombolysis, patients were divided into HT group (18 cases and non-HT group (125 cases. Single factor analysis was used to assess differences in HAT and SEDAN score scales and related risk factors of ischemic stroke in 2 groups, and further Logistic regression analysis was used to investigate independent predictors of HT. Receiver operating characteristic (ROC curve was used to evaluate the sensitivity and specificity of HAT and SEDAN score scales in predicting HT.  Results Univariate Logistic regression analysis showed that history of atrial fibrillation (AF, admission systolic blood pressure (SBP, admission blood glucose level, early low density of head CT, thrombolytic time window, National Institute of Health Stroke Scale (NIHSS, HAT and SEDAN scores were all risk factors for HT after thrombolysis (P < 0.05, for all. Multivariate Logistic regression analysis showed that history of AF (OR = 1.677, 95% CI: 1.332-2.111; P = 0.000, admission SBP (OR = 1.102, 95% CI: 1.009-1.204; P = 0.031, admission blood glucose level (OR = 1.870, 95% CI: 1.119-3.125; P = 0.017, thrombolysis time window (OR = 1.030, 95%CI: 1.009-1.052; P = 0.005, NIHSS score (OR = 1.574, 95%CI: 1.186-2.090; P = 0.002, HAT score (OR = 2.515, 95%CI: 1.273-4.970;P = 0.008 and SEDAN score (OR = 2.413, 95%CI: 1.123-5.185; P = 0.024 were risk factors for HT after thrombolysis. ROC curve analysis showed that HAT score could predict HT with 94.40% sensitivity and 41.60% specificity, and area under curve (AUC was 0.70. SEDAN

  18. Atmospheric circulation of eccentric hot Jupiter HAT-P-2B

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, Nikole K. [Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Showman, Adam P. [Department of Planetary Sciences and Lunar and Planetary Laboratory, The University of Arizona, Tucson, AZ 85721 (United States); Fortney, Jonathan J. [Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States); Knutson, Heather A. [Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125 (United States); Marley, Mark S., E-mail: nklewis@mit.edu [NASA Ames Research Center 245-3, Moffett Field, CA 94035 (United States)

    2014-11-10

    The hot Jupiter HAT-P-2b has become a prime target for Spitzer Space Telescope observations aimed at understanding the atmospheric response of exoplanets on highly eccentric orbits. Here we present a suite of three-dimensional atmospheric circulation models for HAT-P-2b that investigate the effects of assumed atmospheric composition and rotation rate on global scale winds and thermal patterns. We compare and contrast atmospheric models for HAT-P-2b, which assume one and five times solar metallicity, both with and without TiO/VO as atmospheric constituents. Additionally we compare models that assume a rotation period of half, one, and two times the nominal pseudo-synchronous rotation period. We find that changes in assumed atmospheric metallicity and rotation rate do not significantly affect model predictions of the planetary flux as a function of orbital phase. However, models in which TiO/VO are present in the atmosphere develop a transient temperature inversion between the transit and secondary eclipse events that results in significant variations in the timing and magnitude of the peak of the planetary flux compared with models in which TiO/VO are omitted from the opacity tables. We find that no one single atmospheric model can reproduce the recently observed full orbit phase curves at 3.6, 4.5 and 8.0 μm, which is likely due to a chemical process not captured by our current atmospheric models for HAT-P-2b. Further modeling and observational efforts focused on understanding the chemistry of HAT-P-2b's atmosphere are needed and could provide key insights into the interplay between radiative, dynamical, and chemical processes in a wide range of exoplanet atmospheres.

  19. Alternating sequential operators from center-surround top-hat transform

    Science.gov (United States)

    Bai, Xiangzhi

    2013-06-01

    Constructing alternating sequential operators is the important way of improving the efficiency of morphological operations for image processing. And, the alternating sequential filters (ASFs), which are constructed by using the morphological opening and closing, have been the important alternating sequential operators of mathematical morphology. However, because the morphological opening and closing operations have the defect of image detail smoothing, ASFs could not perform very well for some detail preserving image processing applications. Center-surround top-hat transform has been proposed through reconstructing the used structuring elements following the properties of image regions, which ensures that the main operations in center-surround top-hat transform could protect some image details; and, the main operations in center-surround top-hat transform achieve the similar function as opening and closing for bright and black feature extraction. Therefore, based on center-surround top-hat transform, some new and effective alternating sequential operators may be constructed. In light of this, a new class of alternating sequential operators with improved performance based on the center-surround top-hat transform is proposed in this paper. The definition and properties of the new alternating sequential operators are given and analyzed. Also, an application of impulsive noise suppression is used to show the improved performance of the new alternating sequential operators comparing with ASFs. Moreover, because the new alternating sequential operators constructed in this paper have some superiorities over ASFs, they could be also widely used in different applications in which the ASFs perform well, which indicates that the new alternating sequential operators may be also a class of useful morphological operators for image analysis.

  20. Electronic and thermoelectric properties of Mexican hat bands in van-der-Waals materials

    Science.gov (United States)

    Wickramaratne, Darshana; Zahid, Ferdows; Lake, Roger

    2015-03-01

    Mexican hat dispersions are relatively common in few-layer two-dimensional materials. In one to four monolayers of the group-III chalcogenides (GaS, GaSe, InS, InSe) and Bi2Se3 the valence band undergoes a band inversion from a parabolic to an inverted Mexican hat dispersion as the film thickness is reduced from bulk to a single monolayer. The band inversion is robust against changes in stacking order, omission or inclusion of spin-orbit coupling and the choice of functional. The Mexican hat dispersion results in a 1/√{ E} singularity in the two-dimensional density of states and a step-function turn on in the density of modes. The largest radius of the ring of states occurs for a single monolayer of each material. The dispersion with the largest radius coincides with the maximum power factor and ZT for a material at room temperature. Ab-initio electronic structure calculations are used with a Landauer approach to calculate the thermoelectric transport coefficients. Analytical models of the Mexican hat and the parabolic dispersions are used for comparison and analysis. Vertically biased bilayer graphene could serve as an experimental test-bed for measuring this effect since the radius of the Mexican hat band edge increases linearly with vertical electric field. Support by the NSF and SRC-NRI Project 2204.001 (NSF-ECCS-1124733), FAME, one of six centers of STARnet, a SRC program sponsored by MARCO and DARPA and the use of XSEDE NSF Grant # OCI-1053575.

  1. How a hat may affect 3-month-olds' recognition of a face: an eye-tracking study.

    Science.gov (United States)

    Bulf, Hermann; Valenza, Eloisa; Turati, Chiara

    2013-01-01

    Recent studies have shown that infants' face recognition rests on a robust face representation that is resilient to a variety of facial transformations such as rotations in depth, motion, occlusion or deprivation of inner/outer features. Here, we investigated whether 3-month-old infants' ability to represent the invariant aspects of a face is affected by the presence of an external add-on element, i.e. a hat. Using a visual habituation task, three experiments were carried out in which face recognition was investigated by manipulating the presence/absence of a hat during face encoding (i.e. habituation phase) and face recognition (i.e. test phase). An eye-tracker system was used to record the time infants spent looking at face-relevant information compared to the hat. The results showed that infants' face recognition was not affected by the presence of the external element when the type of the hat did not vary between the habituation and test phases, and when both the novel and the familiar face wore the same hat during the test phase (Experiment 1). Infants' ability to recognize the invariant aspects of a face was preserved also when the hat was absent in the habituation phase and the same hat was shown only during the test phase (Experiment 2). Conversely, when the novel face identity competed with a novel hat, the hat triggered the infants' attention, interfering with the recognition process and preventing the infants' preference for the novel face during the test phase (Experiment 3). Findings from the current study shed light on how faces and objects are processed when they are simultaneously presented in the same visual scene, contributing to an understanding of how infants respond to the multiple and composite information available in their surrounding environment.

  2. How a hat may affect 3-month-olds' recognition of a face: an eye-tracking study.

    Directory of Open Access Journals (Sweden)

    Hermann Bulf

    Full Text Available Recent studies have shown that infants' face recognition rests on a robust face representation that is resilient to a variety of facial transformations such as rotations in depth, motion, occlusion or deprivation of inner/outer features. Here, we investigated whether 3-month-old infants' ability to represent the invariant aspects of a face is affected by the presence of an external add-on element, i.e. a hat. Using a visual habituation task, three experiments were carried out in which face recognition was investigated by manipulating the presence/absence of a hat during face encoding (i.e. habituation phase and face recognition (i.e. test phase. An eye-tracker system was used to record the time infants spent looking at face-relevant information compared to the hat. The results showed that infants' face recognition was not affected by the presence of the external element when the type of the hat did not vary between the habituation and test phases, and when both the novel and the familiar face wore the same hat during the test phase (Experiment 1. Infants' ability to recognize the invariant aspects of a face was preserved also when the hat was absent in the habituation phase and the same hat was shown only during the test phase (Experiment 2. Conversely, when the novel face identity competed with a novel hat, the hat triggered the infants' attention, interfering with the recognition process and preventing the infants' preference for the novel face during the test phase (Experiment 3. Findings from the current study shed light on how faces and objects are processed when they are simultaneously presented in the same visual scene, contributing to an understanding of how infants respond to the multiple and composite information available in their surrounding environment.

  3. Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions.

    Science.gov (United States)

    Slamecka, Jaroslav; Salimova, Lilia; McClellan, Steven; van Kelle, Mathieu; Kehl, Debora; Laurini, Javier; Cinelli, Paolo; Owen, Laurie; Hoerstrup, Simon P; Weber, Benedikt

    2016-01-01

    Amniotic fluid stem cells (AFSC) represent an attractive potential cell source for fetal and pediatric cell-based therapies. However, upgrading them to pluripotency confers refractoriness toward senescence, higher proliferation rate and unlimited differentiation potential. AFSC were observed to rapidly and efficiently reacquire pluripotency which together with their easy recovery makes them an attractive cell source for reprogramming. The reprogramming process as well as the resulting iPSC epigenome could potentially benefit from the unspecialized nature of AFSC. iPSC derived from AFSC also have potential in disease modeling, such as Down syndrome or β-thalassemia. Previous experiments involving AFSC reprogramming have largely relied on integrative vector transgene delivery and undefined serum-containing, feeder-dependent culture. Here, we describe non-integrative oriP/EBNA-1 episomal plasmid-based reprogramming of AFSC into iPSC and culture in fully chemically defined xeno-free conditions represented by vitronectin coating and E8 medium, a system that we found uniquely suited for this purpose. The derived AF-iPSC lines uniformly expressed a set of pluripotency markers Oct3/4, Nanog, Sox2, SSEA-1, SSEA-4, TRA-1-60, TRA-1-81 in a pattern typical for human primed PSC. Additionally, the cells formed teratomas, and were deemed pluripotent by PluriTest, a global expression microarray-based in-silico pluripotency assay. However, we found that the PluriTest scores were borderline, indicating a unique pluripotent signature in the defined condition. In the light of potential future clinical translation of iPSC technology, non-integrating reprogramming and chemically defined culture are more acceptable.

  4. HAT: Hypergeometric Analysis of Tiling-arrays with application to promoter-GeneChip data

    Directory of Open Access Journals (Sweden)

    Wouters Bas J

    2010-05-01

    Full Text Available Abstract Background Tiling-arrays are applicable to multiple types of biological research questions. Due to its advantages (high sensitivity, resolution, unbiased, the technology is often employed in genome-wide investigations. A major challenge in the analysis of tiling-array data is to define regions-of-interest, i.e., contiguous probes with increased signal intensity (as a result of hybridization of labeled DNA in a region. Currently, no standard criteria are available to define these regions-of-interest as there is no single probe intensity cut-off level, different regions-of-interest can contain various numbers of probes, and can vary in genomic width. Furthermore, the chromosomal distance between neighboring probes can vary across the genome among different arrays. Results We have developed Hypergeometric Analysis of Tiling-arrays (HAT, and first evaluated its performance for tiling-array datasets from a Chromatin Immunoprecipitation study on chip (ChIP-on-chip for the identification of genome-wide DNA binding profiles of transcription factor Cebpa (used for method comparison. Using this assay, we can refine the detection of regions-of-interest by illustrating that regions detected by HAT are more highly enriched for expected motifs in comparison with an alternative detection method (MAT. Subsequently, data from a retroviral insertional mutagenesis screen were used to examine the performance of HAT among different applications of tiling-array datasets. In both studies, detected regions-of-interest have been validated with (qPCR. Conclusions We demonstrate that HAT has increased specificity for analysis of tiling-array data in comparison with the alternative method, and that it accurately detects regions-of-interest in two different applications of tiling-arrays. HAT has several advantages over previous methods: i as there is no single cut-off level for probe-intensity, HAT can detect regions-of-interest at various thresholds, ii it can

  5. HAT-P-7: A Retrograde or Polar Orbit, and a Third Body

    Science.gov (United States)

    Winn, Joshua N.; Johnson, John Asher; Albrecht, Simon; Howard, Andrew W.; Marcy, Geoffrey W.; Crossfield, Ian J.; Holman, Matthew J.

    2009-01-01

    We showed that the exoplanet HAT-P-7b has an extremely tilted orbit, with a true angle of at least 86 degrees with respect to its parent star's equatorial plane, and a strong possibility of retrograde motion. We also report evidence for an additional planet or companion star. The Rossiter-McLaughlin effect was found to be a blueshift during the first half of the transit and a redshift during the second half, an inversion of the usual pattern, implying that the angle between the sky-projected orbital and stellar angular momentum vectors is 182.5 plus or minus 9.4 degrees. The third body is implicated by excess RV variation of the host star over 2 yr. Some possible explanations for the tilted orbit of HAT-P-7b are a close encounter with another planet, the Kozai effect, and resonant capture by an inward-migrating outer planet.

  6. Kepler's optical phase curve of the exoplanet HAT-P-7b.

    Science.gov (United States)

    Borucki, W J; Koch, D; Jenkins, J; Sasselov, D; Gilliland, R; Batalha, N; Latham, D W; Caldwell, D; Basri, G; Brown, T; Christensen-Dalsgaard, J; Cochran, W D; DeVore, E; Dunham, E; Dupree, A K; Gautier, T; Geary, J; Gould, A; Howell, S; Kjeldsen, H; Lissauer, J; Marcy, G; Meibom, S; Morrison, D; Tarter, J

    2009-08-01

    Ten days of photometric data were obtained during the commissioning phase of the Kepler mission, including data for the previously known giant transiting exoplanet HAT-P-7b. The data for HAT-P-7b show a smooth rise and fall of light from the planet as it orbits its star, punctuated by a drop of 130 +/- 11 parts per million in flux when the planet passes behind its star. We interpret this as the phase variation of the dayside thermal emission plus reflected light from the planet as it orbits its star and is occulted. The depth of the occultation is similar in photometric precision to the detection of a transiting Earth-size planet for which the mission was designed.

  7. Potential ground water resources of Hat Yai Basin in Peninsular Thailand by gravity study

    Directory of Open Access Journals (Sweden)

    Warawutti Lohawijarn

    2005-05-01

    Full Text Available Residual gravity anomaly with a minimum of about -140 mm s-2 with approximately NS trend and a limited axial length was observed over Hat Yai Basin in Peninsular Thailand. The modeled Hat Yai basin is about 1 km deep at its deepest, 60 km long and 20 km wide. The porosity of basin sediment and the amount of potential ground water reserves within the basin are estimated to be 39% and 121.7±0.8 km3 respectively, assuming full saturation. Within the topmost 80 m of ground where the present extraction is concentrated, the estimated ground water reserve is 12.5±0.5 km3.

  8. High multiplicity processes at NLO with BlackHat and Sherpa

    CERN Document Server

    Bern, Zvi; Dixon, Lance J; Hoeche, Stefan; Cordero, Fernando Febres; Ita, Harald; Kosower, David; Maître, Daniel

    2012-01-01

    In this contribution we review recent progress with fixed-order QCD predictions for the production of a vector boson in association with jets at hadron colliders, using the programs BlackHat and SHERPA. We review general features of next-to-leading-order (NLO) predictions for the production of a massive vector boson in association with four jets. We also discuss how precise descriptions of vector-boson production can be applied to the determination of backgrounds to new physics signals. Here we focus on data-driven backgrounds to a missing-energy-plus-jets search performed by CMS. Finally, we review recent progress in developing theoretical tools for high-multiplicity loop-computation within the BlackHat-library. In particular, we discuss methods for handling the color degrees of freedom in multi-jet predictions at NLO.

  9. Study on the Kind and Character of Mongol Hats in Yuan Dynasty

    Institute of Scientific and Technical Information of China (English)

    JIA Xi-zeng

    2007-01-01

    In the thirteenth century the Mongols created a vast, transcontinental empire that intensified cultural, art,craftwork, garment and commercial contact throughout Eurasia. Hats were so indispensable parts of formal Mongol dress that were seen as a symbol of Mongol traditional garment. Roughly Mongolian men's headgear can be divided into two groups, which are Li and Mao. In addition,Mongolian women's Gu-gu-gwan, a kind of bonnet, was the head-dress for the empress, concubines of the emperor, and wife of high ranking ministers. This study, based on the analysis of image resources and existing evidence, discusses the hats of Yuan period in the context of its group, origin, decoration and cultural influences.

  10. Origin of the Napoleon's hat nebula around SN1987A and implications for the progenitor

    Science.gov (United States)

    Podsiadlowski, Ph.; Fabian, A. C.; Stevens, I. R.

    1991-11-01

    A simple geometrical model for the emission nebula around SN1987A, whose morphology has been likened to Napoleon's hat, is presented. The model consists of a ring and a truncated double cone. When the effects of light travel time are included, the model reproduces the important topological structures of the nebula and makes detailed quantitative predictions for its future appearance. In particular, the hat-shaped northern rim is simply explained as the interaction of the light front with the northern cone. To explain the origin of the double cone, it is argued that the progenitor of SN1987A was in a binary system: its strong wind, colliding with a weaker wind from the companion star, created an asymptotic shock surface that was spread out into the required geometry by the rotation of the binary.

  11. First Evidence of a Retrograde Orbit of Transiting Exoplanet HAT-P-7b

    CERN Document Server

    Narita, Norio; Hirano, Teruyuki; Tamura, Motohide

    2009-01-01

    We present the first evidence of a retrograde orbit of the transiting exoplanet HAT-P-7b. The discovery is based on a measurement of the Rossiter-McLaughlin effect with the Subaru HDS during a transit of HAT-P-7b, occured on UT 2008 May 30. Our model shows that the spin-orbit alignment angle of this planet is $\\lambda = -132.6^{\\circ} (+12.6^{\\circ}, -21.5^{\\circ})$. The existence of such a retrograde planet had been predicted by recent planetary migration models considering planet-planet scattering processes or the Kozai migration. Our finding provides an important milestone that supports such dynamic migration theories.

  12. HAT-P-6b: A Hot Jupiter transiting a bright F star

    CERN Document Server

    Noyes, R W; Torres, G; Pal, A; Kovacs, Geza; Latham, D W; Fernández, J M; Fischer, D A; Butler, R P; Marcy, G W; Sipocz, B; Esquerdo, G A; Kovacs, Gabor; Sasselov, D D; Sato, B; Stefanik, R; Holman, M; Lázár, J; Papp, I; Sari, P

    2007-01-01

    In the ongoing HATNet survey we have detected a giant planet, with radius 1.33 +/- 0.06 RJup and mass 1.06 +/- 0.12 MJup, transiting the bright (V = 10.5) star GSC 03466-00819. The planet is in a circular orbit with period 3.852985 +/- 0.000005 days and mid-transit epoch 2,454,035.67575 +/- 0.00028 (HJD). The parent star is a late F star with mass 1.29 +/- 0.06 Msun, radius 1.46 +/- 0.06 Rsun, Teff ~ 6570 +/- 80 K, [Fe=H] = -0.13 +/- 0.08 and age ~ 2.3+/-^{0.5}_{0.7}Gy. With this radius and mass, HAT-P-6b has somewhat larger radius than theoretically expected. We describe the observations and their analysis to determine physical properties of the HAT-P-6 system, and briefly discuss some implications of this finding.

  13. High-power Er:YAG laser with quasi-top-hat output beam.

    Science.gov (United States)

    Kim, J W; Mackenzie, J I; Hayes, J R; Clarkson, W A

    2012-05-01

    A simple method for simultaneously exciting the fundamental (TEM00) transverse mode and first order Laguerre-Gaussian (LG01) donut mode in an end-pumped solid-state laser to yield a quasi-top-hat output beam is reported. This approach has been applied to an Er:YAG laser, in-band pumped by an Er,Yb fiber laser, yielding 9.6 W of continuous-wave output at 1645 nm in a top-hat-like beam with beam propagation factor (M2)<2.1 for 24 W of incident pump power at 1532 nm. The corresponding slope efficiency with respect to incident pump power was 49%. The prospects of further scaling of output power and improved overall efficiency are considered.

  14. Research on the Procedure for Computer-Aided Pattern Design of Uygur's Flowered Hats

    Directory of Open Access Journals (Sweden)

    Shuxian Liu

    2013-05-01

    Full Text Available Xinjiang Uygur’s flowered hats is famous all over the world, it leads the world of headwear of variou nationalities within China by the variety of styles, the various ways of how to emroider pattern design and exquisite manufacture. It is unusual even in national costumes art of the world. The poupose of this study is to analyze Uygur's headwear, study the rules of Uygur's headwear pattern, analyze the characteristic of the basic pattern, research on the procedure for computer-aided pattern design of Uygur's flowered hat, explore the application of computer-aided design in traditional costume's pattern design and to realize application exploration of computer-aided design in designing of costume and dress adornment.

  15. Role Boundaries and Paying Back: 'Switching Hats' in Participant Observation.

    Science.gov (United States)

    Wade, Jacqueline E.

    This paper focuses on the technique of role-switching as a workable field relationship tactic in the observer's work site. It describes the nature of subject use of the observer's dual status during the research activity, and conceptualizes the relationship between this type of researcher-role management and the premise of role reciprocity in…

  16. RSRM top hat cover simulator lightning test, volume 2. Appendix A: Resistance measurements. Appendix B: Lightning test data plots

    Science.gov (United States)

    1990-01-01

    Resistance measurements are given in graphical for when a simulated lightning discharge strikes on an exposed top hat cover simulator. The test sequence was to measure the electric and magnetic fields induced inside a redesigned solid rocket motor case.

  17. Conformal blocks related to the R-R states in the \\hat c =1 SCFT

    CERN Document Server

    Hadasz, Leszek; Suchanek, Paulina

    2007-01-01

    We derive an explicit form of a family of four-point Neveu-Schwarz blocks with $\\hat c =1,$ external weights $\\Delta_i = 1/8$ and arbitrary intermediate weight. The derivation is based on a set of identities obeyed in the free superscalar theory by correlation functions of fields satisfying Ramond condition with respect to the bosonic (dimension 1) and the fermionic (dimension 1/2) currents.

  18. HAT-P-26b: A Neptune-mass Exoplanet with Primordial Solar Heavy Element Abundance

    Science.gov (United States)

    Wakeford, Hannah R.; Sing, David K.; Kataria, Tiffany; Deming, Drake; Nikolov, Nikolay; Lopez, Eric; Tremblin, Pascal; Skalid Amundsen, David; Lewis, Nikole K.; Mandell, Avi; Fortney, Jonathan J.; Knutson, Heather; Benneke, Björn; Evans, Tom M.

    2017-01-01

    A trend in giant planet mass and atmospheric heavy elemental abundance was first noted last century from observations of planets in our own solar system. These four data points from Jupiter, Saturn, Uranus, and Neptune have served as a corner stone of planet formation theory. Here we add another point in the mass-metallicity trend from a detailed observational study of the extrasolar planet HAT-P-26b, which inhabits the critical mass regime near Neptune and Uranus. Neptune-sized worlds are among the most common planets in our galaxy and frequently exist in orbital periods very different from that of our own solar system ice giants. Atmospheric studies are the principal window into these worlds, and thereby into their formation and evolution, beyond those of our own solar system. Using the Hubble Space Telescope and Spitzer, from the optical to the infrared, we conducted a detailed atmospheric study of the Neptune-mass exoplanet HAT-P-26b over 0.5 to 4.5 μm. We detect prominent H2O absorption at 1.4 μm to 525 ppm in the atmospheric transmission spectrum. We determine that HAT-P-26b’s atmosphere is not rich in heavy elements (≈1.8×solar), which goes distinctly against the solar system mass-metallicity trend. This likely indicates that HAT-P-26b’s atmosphere is primordial and obtained its gaseous envelope late in its disk lifetime with little contamination from metal-rich planetesimals.

  19. HAT-P-26b: A Neptune-mass Exoplanet with Primordial Solar Heavy Element Abundance

    Science.gov (United States)

    Wakeford, Hannah; Sing, David; Deming, Drake; Kataria, Tiffany; Lopez, Eric

    2016-10-01

    A trend in giant planet mass and atmospheric heavy elemental abundance was first noted last century from observations of planets in our own solar system. These four data points from Jupiter, Saturn, Uranus, and Neptune have served as a corner stone of planet formation theory. Here we add another point in the mass-metallicity trend from a detailed observational study of the extrasolar planet HAT-P-26b, which inhabits the critical mass regime near Neptune and Uranus. Neptune-sized worlds are among the most common planets in our galaxy and frequently exist in orbital periods very different from that of our own solar system ice giants. Atmospheric studies are the principal window into these worlds, and thereby into their formation and evolution, beyond those of our own solar system. Using the Hubble Space Telescope and Spitzer, from the optical to the infrared, we conducted a detailed atmospheric study of the Neptune-mass exoplanet HAT-P-26b over 0.5 to 4.5 μm. We detect prominent H2O absorption at 1.4 μm to 525 ppm in the atmospheric transmission spectrum. We determine that HAT-P-26b's atmosphere is not rich in heavy elements (≈1.8×solar), which goes distinctly against the solar system mass-metallicity trend. This likely indicates that HAT-P-26b's atmosphere is primordial and obtained its gaseous envelope late in its disk lifetime with little contamination from metal-rich planetesimals.

  20. Intensity and Pattern of Land Surface Temperature in Hat Yai City, Thailand

    OpenAIRE

    Poonyanuch RUTHIRAKO; Rotchanatch DARNSAWASDI; Wichien CHATUPOTE

    2014-01-01

    Land Surface Temperature (LST) is an important factor in global climate. LST is governed by surface heat fluxes, which are affected by urbanization. In order to understand urban climate, LST needs to be examined. This study aimed to investigate the intensity and pattern of LST and examine the relationships between LST and the characteristics of urban land use, indices, and population density in Hat Yai City. Landsat 5TM images were used for interpretation of land use characteristics and deriv...

  1. Detection of Point Sources in Cosmic Ray Maps using the Mexican Hat Wavelet Family

    CERN Document Server

    Batista, Rafael Alves; Daniel, Bruno; 10.1142/S0218301311040608

    2012-01-01

    An analysis of the sensitivity of gaussian and mexican hat wavelet family filters to the detection of point sources of ultra-high energy cosmic rays was performed. A source embedded in a background was simulated and the number of events and amplitude of this source was varied aiming to check the sensitivity of the method to detect faint sources with low statistic of events.

  2. A spin-orbit alignment for the hot Jupiter HATS-3b

    Energy Technology Data Exchange (ETDEWEB)

    Addison, B. C.; Tinney, C. G.; Wright, D. J. [Exoplanetary Science Group, School of Physics, University of New South Wales, Sydney, NSW 2052 (Australia); Bayliss, D., E-mail: b.addison@unsw.edu.au [Research School of Astronomy and Astrophysics, Australian National University, Canberra, ACT 2611 (Australia)

    2014-09-10

    We have measured the alignment between the orbit of HATS-3b (a recently discovered, slightly inflated Hot Jupiter) and the spin axis of its host star. Data were obtained using the CYCLOPS2 optical-fiber bundle and its simultaneous calibration system feeding the UCLES spectrograph on the Anglo-Australian Telescope. The sky-projected spin-orbit angle of λ = 3° ± 25° was determined from spectroscopic measurements of the Rossiter-McLaughlin effect. This is the first exoplanet discovered through the HATSouth transit survey to have its spin-orbit angle measured. Our results indicate that the orbital plane of HATS-3b is consistent with being aligned to the spin axis of its host star. The low obliquity of the HATS-3 system, which has a relatively hot mid F-type host star, agrees with the general trend observed for Hot Jupiter host stars with effective temperatures >6250 K to have randomly distributed spin-orbit angles.

  3. HAT-P-25b: a Hot-Jupiter Transiting a Moderately Faint G Star

    CERN Document Server

    Quinn, S N; Hartman, J; Torres, G; Kovács, G; Latham, D W; Noyes, R W; Fischer, D A; Johnson, J A; Marcy, G W; Howard, A W; Szentgyorgyi, A; Fürész, G; Buchhave, L A; Béky, B; Sasselov, D D; Stefanik, R P; Perumpilly, G; Everett, M; Lázár, J; Papp, I; Sári, P

    2010-01-01

    We report the discovery of HAT-P-25b, a transiting extrasolar planet orbiting the V = 13.19 G5 dwarf star GSC 1788-01237, with a period P = 3.652836 +/- 0.000019 days, transit epoch Tc = 2455176.85173 +/- 0.00047 (BJD), and transit duration 0.1174 +/- 0.0017 days. The host star has mass of 1.01 +/- 0.03 M(Sun), radius of 0.96 +(0.05)-(0.04) R(Sun), effective temperature 5500 +/- 80 K, and metallicity [Fe/H] = +0.31 +/- 0.08. The planetary companion has a mass of 0.567 +/- 0.022 M(Jup), and radius of 1.190 +(0.081)-(0.056) R(Jup) yielding a mean density of 0.42 +/- 0.07 g cm-3. Comparing these observations with recent theoretical models, we find that HAT-P-25b is consistent with a hydrogen-helium dominated gas giant planet with negligible core mass and age 3.2 +/- 2.3 Gyr. The properties of HAT-P-25b support several previously observed correlations for planets in the mass range 0.4 < M < 0.7 M(Jup), including those of core mass vs. metallicity, planet radius vs. equilibrium temperature, and orbital perio...

  4. HATS-18 b: An Extreme Short--Period Massive Transiting Planet Spinning Up Its Star

    CERN Document Server

    Penev, Dr Kaloyan M; Bakos, Dr Gaspar A; Ciceri, Ms Simona; Brahm, Dr Rafael; Bayliss, Dr Daniel; Bento, Joao; Jord'an, Andr'es; Csubry, Mr Zoltan; Bhatti, W; de Val-Borro, Miguel; Espinoza, Mr Néstor; Zhou, Dr George; Mancini, Dr Luigi; Rabus, Dr Markus; Suc, Vincent; Henning, Thomas; Schmidt, Prof Brian P; Noyes, Dr Robert W; L'az'ar, J; Papp, Istvan; S'ari, P

    2016-01-01

    We report the discovery by the HATSouth network of HATS-18 b: a 1.980 +/- 0.077 Mj, 1.337 +0.102 -0.049 Rj planet in a 0.8378 day orbit, around a solar analog star (mass 1.037 +/- 0.047 Msun, and radius 1.020 +0.057 -0.031 Rsun) with V=14.067 +/- 0.040 mag. The high planet mass, combined with its short orbital period, implies strong tidal coupling between the planetary orbit and the star. In fact, given its inferred age, HATS-18 shows evidence of significant tidal spin up, which together with WASP-19 (a very similar system) allows us to constrain the tidal quality factor for Sun-like stars to be in the range 6.5 <= lg(Q*/k_2) <= 7 even after allowing for extremely pessimistic model uncertainties. In addition, the HATS-18 system is among the best systems (and often the best system) for testing a multitude of star--planet interactions, be they gravitational, magnetic or radiative, as well as planet formation and migration theories.

  5. HATS-4b: A Dense Hot-Jupiter Transiting a Super Metal-Rich G Star

    CERN Document Server

    Jordán, A; Bakos, G Á; Bayliss, D; Penev, K; Hartman, J D; Zhou, G; Mancini, L; Mohler-Fischer, M; Ciceri, S; Sato, B; Csubry, Z; Rabus, M; Suc, V; Espinoza, N; Bhatti, W; Borro, M de Val; Buchhave, L; Csák, B; Henning, T; Schmidt, B; Tan, T G; Noyes, R W; Béky, B; Butler, R P; Shectman, S; Crane, J; Thompson, I; Williams, A; Martin, R; Contreras, C; Lázár, J; Papp, I; Sári, P

    2014-01-01

    We report the discovery by the HATSouth survey of HATS-4b, an extrasolar planet transiting a V=13.46 mag G star. HATS-4b has a period of P = 2.5167 d, mass of Mp = 1.32 Mj, radius of Rp = 1.02 Rj and density of rho_p = 1.55 +- 0.16 g/cm^3 ~ 1.24 rhoj. The host star has a mass of 1.00 Msun, a radius of 0.92 Rsun and a very high metallicity [Fe/H]= 0.43 +- 0.08. HATS-4b is among the densest known planets with masses between 1-2 Mj and is thus likely to have a significant content of heavy elements of the order of 75 Mearth. In this paper we present the data reduction, radial velocity measurement and stellar classification techniques adopted by the HATSouth survey for the CORALIE spectrograph. We also detail a technique to estimate simultaneously vsini and macroturbulence using high resolution spectra.

  6. iHAT: interactive Hierarchical Aggregation Table for Genetic Association Data

    Directory of Open Access Journals (Sweden)

    Heinrich Julian

    2012-05-01

    Full Text Available Abstract In the search for single-nucleotide polymorphisms which influence the observable phenotype, genome wide association studies have become an important technique for the identification of associations between genotype and phenotype of a diverse set of sequence-based data. We present a methodology for the visual assessment of single-nucleotide polymorphisms using interactive hierarchical aggregation techniques combined with methods known from traditional sequence browsers and cluster heatmaps. Our tool, the interactive Hierarchical Aggregation Table (iHAT, facilitates the visualization of multiple sequence alignments, associated metadata, and hierarchical clusterings. Different color maps and aggregation strategies as well as filtering options support the user in finding correlations between sequences and metadata. Similar to other visualizations such as parallel coordinates or heatmaps, iHAT relies on the human pattern-recognition ability for spotting patterns that might indicate correlation or anticorrelation. We demonstrate iHAT using artificial and real-world datasets for DNA and protein association studies as well as expression Quantitative Trait Locus data.

  7. On Variable Geometric Factor Systems for Top-Hat Electrostatic Space Plasma Analyzers

    Science.gov (United States)

    Kataria, Dhiren O.; Collinson, Glyn A.

    2010-01-01

    Even in the relatively small region of space that is the Earth's magnetosphere, ion and electron fluxes can vary by several orders of magnitude. Top-hat electrostatic analyzers currently do not possess the dynamic range required to sample plasma under all conditions. The purpose of this study was to compare, through computer simulation, three new electrostatic methods that would allow the sensitivity of a sensor to be varied through control of its geometric factor (GF) (much like an aperture on a camera). The methods studied were inner filter plates, split hemispherical analyzer (SHA) and top-cap electrode. This is the first discussion of the filter plate concept and also the first study where all three systems are studied within a common analyzer design, so that their relative merits could be fairly compared. Filter plates were found to have the important advantage that they facilitate the reduction in instrument sensitivity whilst keeping all other instrument parameters constant. However, it was discovered that filter plates have numerous disadvantages that make such a system impracticable for a top-hat electrostatic analyzer. It was found that both the top-cap electrode and SHA are promising variable geometric factor system (VGFS) concepts for implementation into a top-hat electrostatic analyzer, each with distinct advantages over the other.

  8. Formulating robust linear regression estimation as a one-class LDA criterion: discriminative hat matrix.

    Science.gov (United States)

    Dufrenois, F; Noyer, J C

    2013-02-01

    Linear discriminant analysis, such as Fisher's criterion, is a statistical learning tool traditionally devoted to separating a training dataset into two or even several classes by the way of linear decision boundaries. In this paper, we show that this tool can formalize the robust linear regression problem as a robust estimator will do. More precisely, we develop a one-class Fischer's criterion in which the maximization provides both the regression parameters and the separation of the data in two classes: typical data and atypical data or outliers. This new criterion is built on the statistical properties of the subspace decomposition of the hat matrix. From this angle, we improve the discriminative properties of the hat matrix which is traditionally used as outlier diagnostic measure in linear regression. Naturally, we call this new approach discriminative hat matrix. The proposed algorithm is fully nonsupervised and needs only the initialization of one parameter. Synthetic and real datasets are used to study the performance both in terms of regression and classification of the proposed approach. We also illustrate its potential application to image recognition and fundamental matrix estimation in computer vision.

  9. Infrared image enhancement through contrast enhancement by using multiscale new top-hat transform

    Science.gov (United States)

    Bai, Xiangzhi; Zhou, Fugen; Xue, Bindang

    2011-03-01

    Infrared imaging sensor is sensitive to the variation of imaging environment, which may affect the quality of the obtained images and blur the regions of interest in infrared image. So, it is very important to enhance infrared image. In infrared image, the gray values of the regions of interest are bright or dim image regions, which are different from the surrounding regions. The new top-hat transform could extract image regions which are different from its surrounding regions. In light of this, an infrared image enhancement algorithm through contrast enhancement is proposed in this paper based on multiscale new top-hat transform. Firstly, the multiscale white and black new top-hat transforms are used to extract the multiscale light and dark infrared image regions. Then, the final light and dark infrared image regions for image enhancement are constructed by using the extracted multiscale light and dark infrared image regions. Finally, the contrast of the infrared image is enhanced through a power strategy. Experimental results on different infrared images show that the proposed algorithm could well enhance infrared image and make the possible interested targets brighter, which is very helpful for target detection and recognition.

  10. Asteroseismic inference on the spin-orbit misalignment and stellar parameters of HAT-P-7

    CERN Document Server

    Lund, Mikkel N; Aguirre, Victor Silva; Houdek, Günter; Casagrande, Luca; Van Eylen, Vincent; Campante, Tiago L; Karoff, Christoffer; Kjeldsen, Hans; Albrecht, Simon; Chaplin, William J; Nielsen, Martin Bo; Degroote, Pieter; Davies, Guy R; Handberg, Rasmus

    2014-01-01

    The measurement of obliquities in star-planet systems is of great importance for the understanding of planet system formation and evolution. The bright and well studied HAT-P-7 system is intriguing as several Rossiter-McLaughlin (RM) measurements found a large projected obliquity in this system, but it was so far not possible to determine if the orbit is polar and/or retrograde. The goal of this study is to measure the stellar inclination and hereby the full 3D obliquity of the HAT-P-7 system instead of only the 2D projection as measured by the RM effect. In addition we provide an updated set of stellar parameters for the star. We use the full set of available observations from Kepler spanning Q0-Q17 to produce the power spectrum of HAT-P-7. We extract oscillation mode frequencies via an MCMC peak-bagging routine, and use the results from this to estimate the stellar inclination angle. Combining this with the projected obliquity from RM and the inclination of the orbital plane allows us to determine the stell...

  11. Independent discovery and refined parameters of the transiting exoplanet HAT-P-14b

    CERN Document Server

    Simpson, E K; Brown, D J A; Cameron, A Collier; Pollacco, D; Skillen, I; Stempels, H C; Boisse, I; Faedi, F; Hebrard, G; McCormac, J; Sorensen, P; Street, R A; Bento, J; Bouchy, F; Butters, O W; Enoch, B; Haswell, C A; Hebb, L; Holmes, S; Horne, K; Keenan, F P; Lister, T A; Miller, G R M; Moulds, V; Moutou, C; Norton, A J; Parley, N; Santerne, A; Todd, I; Watson, C A; West, R G; Wheatley, P J

    2010-01-01

    We present SuperWASP observations of HAT-P-14b, a hot Jupiter discovered by Torres et al. The planet was found independently by the SuperWASP team and named WASP-27b after follow-up observations had secured the discovery, but prior to the publication by Torres et al. Our analysis of HAT-P-14/WASP-27 is in good agreement with the values found by Torres et al. and we refine the parameters by combining our datasets. We also provide additional evidence against astronomical false positives. Due to the brightness of the host star, V = 10, HAT-P-14 is an attractive candidate for further characterisation observations. The planet has a high impact parameter, b = 0.907 +/- 0.004, and the primary transit is close to grazing. This could readily reveal small deviations in the orbital parameters indicating the presence of a third body in the system, which may be causing the small but significant orbital eccentricity, e = 0.095 +/- 0.011. The system geometry suggests that the planet narrowly fails to undergo a secondary ecl...

  12. A Possible Tilted Orbit of the Super-Neptune HAT-P-11b

    CERN Document Server

    Hirano, Teruyuki; Shporer, Avi; Sato, Bun'ei; Aoki, Wako; Tamura, Motohide

    2010-01-01

    We report the detection of the Rossiter-McLaughlin effect for the eccentric, super-Neptune exoplanet HAT-P-11b, based on radial velocity measurements taken with HDS mounted on the Subaru 8.2m telescope, and simultaneous photometry with the FTN 2.0m telescope, both located in Hawai'i. The observed radial velocities during a planetary transit of HAT-P-11b show a persistent blue-shift, suggesting a spin-orbit misalignment in the system. The best-fit value for the projected spin-orbit misalignment angle is $\\lambda= 103_{-19}^{+23}$ deg. Our result supports the notion that eccentric exoplanetary systems are likely to have significant spin-orbit misalignment (e.g., HD 80606, WASP-8, WASP-14, WASP-17, and XO-3). This fact suggests that not only hot-Jupiters but also super-Neptunes like HAT-P-11b had once experienced dynamical processes such as planet-planet scattering or the Kozai migration.

  13. Atmospheric Circulation of Eccentric Hot Jupiter HAT-P-2b

    CERN Document Server

    Lewis, Nikole K; Fortney, Jonathan J; Knutson, Heather A; Marley, Mark S

    2014-01-01

    The hot-Jupiter HAT-P-2b has become a prime target for Spitzer Space Telescope observations aimed at understanding the atmospheric response of exoplanets on highly eccentric orbits. Here we present a suite of three-dimensional atmospheric circulation models for HAT-P-2b that investigate the effects of assumed atmospheric composition and rotation rate on global scale winds and thermal patterns. We compare and contrast atmospheric models for HAT-P-2b, which assume one and five times solar metallicity, both with and without TiO/VO as atmospheric constituents. Additionally we compare models that assume a rotation period of half, one, and two times the nominal pseudo-synchronous rotation period. We find that changes in assumed atmospheric metallicity and rotation rate do not significantly affect model predictions of the planetary flux as a function of orbital phase. However, models in which TiO/VO are present in the atmosphere develop a transient temperature inversion between the transit and secondary eclipse even...

  14. Gaussian versus top-hat profile assumptions in integral plume models

    Science.gov (United States)

    Davidson, G. A.

    Numerous integral models describing the behaviour of buoyant plumes released into stratified crossflows have been presented in the literature. One of the differences between these models is the form assumed for the self-similar profile: some models assume a top-hat form while others assume a Gaussian. The differences between these two approaches are evaluated by (a) comparing the governing equations on which Gaussian and top-hat models are based; (b) comparing some typical plume predictions generated by each type of model over a range of model parameters. It is shown that, while the profile assumption does lead to differences in the equations which govern plume variables, the effects of these differences on actual plume predictions is small over the range of parameters of practical interest. Since the predictions of Gaussian and top-hat models are essentially equivalent, it can thus be concluded that the additional physical information incorporated into a Gaussian formulation plays only a minor role in mean plume behaviour, and that the tophat approach, which requires the numerical solution of a simpler set of equations, is adequate for most situations where an integral approach would be used.

  15. Detection of the Secondary Eclipse of Exoplanet HAT P-11b

    Science.gov (United States)

    Barry, R. K.; Deming, L. D.; Bakos, G.; Harrington, J.; Madhusudhan, N.; Noyes, R.; Seager, S.

    2010-01-01

    We have successfully conducted secondary eclipse observations of exoplanet HAT-P-11b using the Spitzer Space Telescope. HAT-P-11b was, until very recently, the smallest transiting extrasolar planet yet found and one of only two known exo-Neptunes. We observed the system at 3.6 microns for a period of 22 hours centered on the anticipated secondary eclipse time, to detect the eclipse and determine its phase. Having detected the secondary eclipse, we are at present making a more focused series of observations in both the 3.6 and 4.5 micron bands to fully characterize it. HAT-P-11b has a period of 4.8878 days, radius of 0.422 RJ, mass of 0.081 MJ and semi-major axis 0.053 AU. Measurements of the secondary eclipse will serve to clarify two key issues; 1) the planetary brightness temperature and the nature of its atmosphere, and 2) the eccentricity of its orbit, with implications for its dynamical evolution. A precise determination of the orbit phase for the secondary eclipse will also be of great utility for Kepler observations of this system at visible wavelengths.

  16. Image registration algorithm using Mexican hat function-based operator and grouped feature matching strategy.

    Directory of Open Access Journals (Sweden)

    Feng Jin

    Full Text Available Feature detection and matching are crucial for robust and reliable image registration. Although many methods have been developed, they commonly focus on only one class of image features. The methods that combine two or more classes of features are still novel and significant. In this work, methods for feature detection and matching are proposed. A Mexican hat function-based operator is used for image feature detection, including the local area detection and the feature point detection. For the local area detection, we use the Mexican hat operator for image filtering, and then the zero-crossing points are extracted and merged into the area borders. For the feature point detection, the Mexican hat operator is performed in scale space to get the key points. After the feature detection, an image registration is achieved by using the two classes of image features. The feature points are grouped according to a standardized region that contains correspondence to the local area, precise registration is achieved eventually by the grouped points. An image transformation matrix is estimated by the feature points in a region and then the best one is chosen through competition of a set of the transformation matrices. This strategy has been named the Grouped Sample Consensus (GCS. The GCS has also ability for removing the outliers effectively. The experimental results show that the proposed algorithm has high registration accuracy and small computational volume.

  17. HATS-4b: A dense hot Jupiter transiting a super metal-rich G star

    Energy Technology Data Exchange (ETDEWEB)

    Jordán, Andrés; Brahm, Rafael; Rabus, M.; Suc, V.; Espinoza, N. [Instituto de Astrofísica, Facultad de Física, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, 7820436 Macul, Santiago (Chile); Bakos, G. Á.; Penev, K.; Hartman, J. D.; Csubry, Z.; Bhatti, W.; De Val Borro, M. [Department of Astrophysical Sciences, Princeton University, NJ 08544 (United States); Bayliss, D.; Zhou, G. [The Australian National University, Canberra (Australia); Mancini, L.; Mohler-Fischer, M.; Ciceri, S.; Csák, B.; Henning, T. [Max Planck Institute for Astronomy, Heidelberg (Germany); Sato, B. [Department of Earth and Planetary Sciences, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8551 (Japan); Buchhave, L. [Niels Bohr Institute, Copenhagen University (Denmark); and others

    2014-08-01

    We report the discovery by the HATSouth survey of HATS-4b, an extrasolar planet transiting a V = 13.46 mag G star. HATS-4b has a period of P ≈ 2.5167 days, mass of M{sub p} ≈ 1.32 M {sub Jup}, radius of R{sub p} ≈ 1.02 R {sub Jup}, and density of ρ {sub p} = 1.55 ± 0.16 g cm{sup –3} ≈1.24 ρ{sub Jup}. The host star has a mass of 1.00 M {sub ☉}, a radius of 0.92 R {sub ☉}, and a very high metallicity [Fe/H]=0.43 ± 0.08. HATS-4b is among the densest known planets with masses between 1 and 2 M {sub J} and is thus likely to have a significant content of heavy elements of the order of 75 M {sub ⊕}. In this paper we present the data reduction, radial velocity measurements, and stellar classification techniques adopted by the HATSouth survey for the CORALIE spectrograph. We also detail a technique for simultaneously estimating vsin i and macroturbulence using high resolution spectra.

  18. Follow-up Observations of the Neptune Mass Transiting Extrasolar Planet Hat-P-11b

    CERN Document Server

    Dittman, Jason A; Green, Elizabeth M; Scuderi, Louis J; Males, Jared R

    2009-01-01

    We have confirmed the existence of the transiting super Neptune extrasolar planet HAT-P-11b. On May 1, 2009 UT the transit of Hat-P-11b was detected at the University of Arizona's 1.55m Kuiper Telescope with 1.7 millimag rms accuracy. We find a central transit time of Tc = 2454952.92534+/-0.00060 BJD; this transit occurred 80+/-73 seconds sooner than previous measurements (71 orbits in the past) would have predicted. Hence, our transit timing rules out large deviations from the ephemeris of Bakos et al. (2009). We measure a slightly larger planetary radius of Rp=0.452+/-0.020 R_Jup (5.07+/-0.22 R_earth) compared to Bakos and co-workers' value of 0.422+/-0.014 R_Jup (4.73+/-0.16 R_earth). Our values confirm that Hat-P-11b is very similar to GJ 436b (the only other known transiting super Neptune) in radius and other bulk properties.

  19. The refined physical parameters of transiting exoplanet system HAT-P-24

    Institute of Scientific and Technical Information of China (English)

    Xiao-Bin Wang; Sheng-Hong Gu; Andrew Collier Cameron; Hong-Bo Tan; Ho-Keung Hui; Chi-Tai Kwok; Bill Yeung

    2013-01-01

    The transiting exoplanet system HAT-P-24 was observed by using CCD cameras at Yunnan Observatory and Hokoon Astronomical Centre,China in 2010 and 2012.In order to enhance the signal to noise ratio of transit events,the observed data are corrected for systematic errors according to Collier Cameron et al.'s coarse decorrelation and Tamuz et al.'s SYSREM algorithms.Three new complete transit light curves are analyzed by means of the Markov chain Monte Carlo technique,and the new physical parameters of the system are derived.They are consistent with the old ones from the discovered paper except for a new larger radius RP =1.364 RJ of HAT-P-24b,which confirms its inflated nature.By combining the five available epochs of mid-transit derived from complete transit light curves,the orbital period of HAT-P-24b is refined to P =3.3552479 d and no obvious transit timing variation signal can be found from these five transit events during 2010-2012.

  20. Reconstruction-by-Dilation and Top-Hat Algorithms for Contrast Enhancement and Segmentation of Microcalcifications in Digital Mammograms

    Science.gov (United States)

    Diaz, Claudia C.

    2007-11-01

    I present some results of contrast enhancement and segmentation of microcalcifications in digital mammograms. These mammograms were obtained from MIAS-minidatabase and using a CR to digitize images. White-top-hat and black-top-hat transformations were used to improve the contrast of images, while reconstruction-by-dilation algorithm was used to emphasize the microcalcifications over the tissues. Segmentation was done using different gradient matrices. These algorithms intended to show some details which were not evident in original images.

  1. Spin-orbit alignment for KELT-7b and HAT-P-56b via Doppler tomography with TRES

    Science.gov (United States)

    Zhou, George; Latham, David W.; Bieryla, Allyson; Beatty, Thomas G.; Buchhave, Lars A.; Esquerdo, Gilbert A.; Berlind, Perry; Calkins, Michael L.

    2016-08-01

    We present Doppler tomographic analyses for the spectroscopic transits of KELT-7b and HAT-P-56b, two hot-Jupiters orbiting rapidly rotating F-dwarf host stars. These include analyses of archival Tillinghast Reflector Echelle Spectrograph (TRES) observations for KELT-7b, and a new TRES transit observation of HAT-P-56b. We report spin-orbit aligned geometries for KELT-7b (2.7° ± 0.6°) and HAT-P-56b (8° ± 2°). The host stars KELT-7 and HAT-P-56 are among some of the most rapidly rotating planet-hosting stars known. We examine the tidal re-alignment model for the evolution of the spin-orbit angle in the context of the spin rates of these stars. We find no evidence that the rotation rates of KELT-7 and HAT-P-56 have been modified by star-planet tidal interactions, suggesting that the spin-orbit angle of systems around these hot stars may represent their primordial configuration. In fact, KELT-7 and HAT-P-56 are two of three systems in supersynchronous, spin-orbit aligned states, where the rotation periods of the host stars are faster than the orbital periods of the planets.

  2. Spin orbit alignment for KELT-7b and HAT-P-56b via Doppler tomography with TRES

    CERN Document Server

    Zhou, George; Bieryla, Allyson; Beatty, Thomas G; Buchhave, Lars A; Esquerdo, Gilbert A; Berlind, Perry; Calkins, Michael L

    2016-01-01

    We present Doppler tomographic analyses for the spectroscopic transits of KELT-7b and HAT-P-56b, two hot-Jupiters orbiting rapidly rotating F-dwarf host stars. These include analyses of archival TRES observations for KELT-7b, and a new TRES transit observation of HAT-P-56b. We report spin-orbit aligned geometries for KELT-7b (2.7 +/- 0.6 deg) and HAT-P-56b (8 +/- 2 deg). The host stars KELT-7 and HAT-P-56 are among some of the most rapidly rotating planet-hosting stars known. We examine the tidal re-alignment model for the evolution of the spin-orbit angle in the context of the spin rates of these stars. We find no evidence that the rotation rates of KELT-7 and HAT-P-56 have been modified by star-planet tidal interactions, suggesting that the spin-orbit angle of systems around these hot stars may represent their primordial configuration. In fact, KELT-7 and HAT-P-56 are two of three systems in super-synchronous, spin-orbit aligned states, where the rotation periods of the host stars are faster than the orbita...

  3. Thermal and Mechanical Buckling Analysis of Hypersonic Aircraft Hat-Stiffened Panels With Varying Face Sheet Geometry and Fiber Orientation

    Science.gov (United States)

    Ko, William L.

    1996-01-01

    Mechanical and thermal buckling behavior of monolithic and metal-matrix composite hat-stiffened panels were investigated. The panels have three types of face-sheet geometry: Flat face sheet, microdented face sheet, and microbulged face sheet. The metal-matrix composite panels have three types of face-sheet layups, each of which is combined with various types of hat composite layups. Finite-element method was used in the eigenvalue extractions for both mechanical and thermal buckling. The thermal buckling analysis required both eigenvalue and material property iterations. Graphical methods of the dual iterations are shown. The mechanical and thermal buckling strengths of the hat-stiffened panels with different face-sheet geometry are compared. It was found that by just microdenting or microbulging of the face sheet, the axial, shear, and thermal buckling strengths of both types of hat-stiffened panels could be enhanced considerably. This effect is more conspicuous for the monolithic panels. For the metal-matrix composite panels, the effect of fiber orientations on the panel buckling strengths was investigated in great detail, and various composite layup combinations offering, high panel buckling strengths are presented. The axial buckling strength of the metal-matrix panel was sensitive to the change of hat fiber orientation. However, the lateral, shear, and thermal buckling strengths were insensitive to the change of hat fiber orientation.

  4. Signatures of rocky planet engulfment in HAT-P-4. Implications for chemical tagging studies

    Science.gov (United States)

    Saffe, C.; Jofré, E.; Martioli, E.; Flores, M.; Petrucci, R.; Jaque Arancibia, M.

    2017-07-01

    Aims: We aim to explore the possible chemical signature of planet formation in the binary system HAT-P-4 by studying the trends of abundance vs. condensation temperature Tc. The star HAT-P-4 hosts a planet detected by transits, while its stellar companion does not have any detected planet. We also study the lithium content, which might shed light on the problem of Li depletion in exoplanet host stars. Methods: We derived for the first time both stellar parameters and high-precision chemical abundances by applying a line-by-line full differential approach. The stellar parameters were determined by imposing ionization and excitation equilibrium of Fe lines, with an updated version of the FUNDPAR program, together with ATLAS9 model atmospheres and the MOOG code. We derived detailed abundances of different species with equivalent widths and spectral synthesis with the MOOG program. Results: The exoplanet host star HAT-P-4 is found to be 0.1 dex more metal rich than its companion, which is one of the highest differences in metallicity observed in similar systems. This could have important implications for chemical tagging studies. We rule out a possible peculiar composition for each star, such as is the case for λ Boötis and δ Scuti, and neither is this binary a blue straggler. The star HAT-P-4 is enhanced in refractory elements relative to volatile when compared to its stellar companion. Notably, the Li abundance in HAT-P-4 is greater than that of its companion by 0.3 dex, which is contrary to the model that explains the Li depletion by the presence of planets. We propose a scenario where at the time of planet formation, the star HAT-P-4 locked the inner refractory material in planetesimals and rocky planets, and formed the outer gas giant planet at a greater distance. The refractories were then accreted onto the star, possibly as a result of the migration of the giant planet. This explains the higher metallicity, the higher Li content, and the negative Tc trend we

  5. ORBITAL PHASE VARIATIONS OF THE ECCENTRIC GIANT PLANET HAT-P-2b

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, Nikole K.; Showman, Adam P. [Department of Planetary Sciences and Lunar and Planetary Laboratory, The University of Arizona, Tucson, AZ 85721 (United States); Knutson, Heather A.; Desert, Jean-Michel; Kao, Melodie [Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125 (United States); Cowan, Nicolas B. [Center for Interdisciplinary Exploration and Research in Astrophysics and Department of Physics and Astronomy, Northwestern University, 2131 Tech Drive, Evanston, IL 60208 (United States); Laughlin, Gregory; Fortney, Jonathan J. [Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States); Burrows, Adam; Bakos, Gaspar A.; Hartman, Joel D. [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08544 (United States); Deming, Drake [Department of Astronomy, University of Maryland, College Park, MD 20742 (United States); Crepp, Justin R. [Department of Physics, University of Notre Dame, Notre Dame, IN 46556 (United States); Mighell, Kenneth J. [National Optical Astronomy Observatories, Tucson, AZ 85726 (United States); Agol, Eric [Department of Astronomy, University of Washington, Seattle, WA 98195 (United States); Charbonneau, David [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Fischer, Debra A. [Department of Astronomy, Yale University, New Haven, CT 06511 (United States); Hinkley, Sasha; Johnson, John Asher [Department of Astrophysics, California Institute of Technology, MC 249-17, Pasadena, CA 91125 (United States); Howard, Andrew W., E-mail: nklewis@mit.edu [Institute for Astronomy, University of Hawaii, 2680 Woodlawn Drive, Honolulu, HI 96822 (United States); and others

    2013-04-01

    We present the first secondary eclipse and phase curve observations for the highly eccentric hot Jupiter HAT-P-2b in the 3.6, 4.5, 5.8, and 8.0 {mu}m bands of the Spitzer Space Telescope. The 3.6 and 4.5 {mu}m data sets span an entire orbital period of HAT-P-2b (P = 5.6334729 d), making them the longest continuous phase curve observations obtained to date and the first full-orbit observations of a planet with an eccentricity exceeding 0.2. We present an improved non-parametric method for removing the intrapixel sensitivity variations in Spitzer data at 3.6 and 4.5 {mu}m that robustly maps position-dependent flux variations. We find that the peak in planetary flux occurs at 4.39 {+-} 0.28, 5.84 {+-} 0.39, and 4.68 {+-} 0.37 hr after periapse passage with corresponding maxima in the planet/star flux ratio of 0.1138% {+-} 0.0089%, 0.1162% {+-} 0.0080%, and 0.1888% {+-} 0.0072% in the 3.6, 4.5, and 8.0 {mu}m bands, respectively. Our measured secondary eclipse depths of 0.0996% {+-} 0.0072%, 0.1031% {+-} 0.0061%, 0.071%{sub -0.013%}{sup +0.029,} and 0.1392% {+-} 0.0095% in the 3.6, 4.5, 5.8, and 8.0 {mu}m bands, respectively, indicate that the planet cools significantly from its peak temperature before we measure the dayside flux during secondary eclipse. We compare our measured secondary eclipse depths to the predictions from a one-dimensional radiative transfer model, which suggests the possible presence of a transient day side inversion in HAT-P-2b's atmosphere near periapse. We also derive improved estimates for the system parameters, including its mass, radius, and orbital ephemeris. Our simultaneous fit to the transit, secondary eclipse, and radial velocity data allows us to determine the eccentricity (e = 0.50910 {+-} 0.00048) and argument of periapse ({omega} = 188. Degree-Sign 09 {+-} 0. Degree-Sign 39) of HAT-P-2b's orbit with a greater precision than has been achieved for any other eccentric extrasolar planet. We also find evidence for a long

  6. HATS-18b: An Extreme Short-period Massive Transiting Planet Spinning Up Its Star

    Science.gov (United States)

    Penev, K.; Hartman, J. D.; Bakos, G. Á.; Ciceri, S.; Brahm, R.; Bayliss, D.; Bento, J.; Jordán, A.; Csubry, Z.; Bhatti, W.; de Val-Borro, M.; Espinoza, N.; Zhou, G.; Mancini, L.; Rabus, M.; Suc, V.; Henning, T.; Schmidt, B.; Noyes, R. W.; Lázár, J.; Papp, I.; Sári, P.

    2016-11-01

    We report the discovery by the HATSouth network of HATS-18b: a 1.980+/- 0.077 {M}{{J}}, {1.337}-0.049+0.102 {R}{{J}} planet in a 0.8378 day orbit, around a solar analog star (mass 1.037+/- 0.047 {M}⊙ and radius {1.020}-0.031+0.057 {R}⊙ ) with V=14.067+/- 0.040 mag. The high planet mass, combined with its short orbital period, implies strong tidal coupling between the planetary orbit and the star. In fact, given its inferred age, HATS-18 shows evidence of significant tidal spin up, which together with WASP-19 (a very similar system) allows us to constrain the tidal quality factor for Sun-like stars to be in the range of 6.5≲ {{log}}10({Q}* /{k}2)≲ 7 even after allowing for extremely pessimistic model uncertainties. In addition, the HATS-18 system is among the best systems (and often the best system) for testing a multitude of star-planet interactions, be they gravitational, magnetic, or radiative, as well as planet formation and migration theories. The HATSouth network is operated by a collaboration consisting of Princeton University (PU), the Max Planck Institute für Astronomie (MPIA), the Australian National University (ANU), and the Pontificia Universidad Católica de Chile (PUC). The station at Las Campanas Observatory (LCO) of the Carnegie Institute is operated by PU in conjunction with PUC, the station at the High Energy Spectroscopic Survey (H.E.S.S.) site is operated in conjunction with MPIA, and the station at Siding Spring Observatory (SSO) is operated jointly with ANU. This paper includes data gathered with the MPG 2.2 m telescope at the ESO Observatory in La Silla. This paper uses observations obtained with facilities of the Las Cumbres Observatory Global Telescope.

  7. Refined System Parameters and TTV Study of Transiting Exoplanetary System HAT-P-20

    Science.gov (United States)

    Sun, Leilei; Gu, Shenghong; Wang, Xiaobin; Collier Cameron, Andrew; Cao, Dongtao; Wang, Yibo; Xiang, Yue; Hui, Ho-Keung; Kwok, Chi-Tai; Yeung, Bill; Ng, Eric; Grau Horta, Ferran

    2017-01-01

    We report new photometric observations of the transiting exoplanetary system HAT-P-20, obtained using CCD cameras at Yunnan Observatories and Ho Koon Nature Education cum Astronomical Centre, China, from 2010 to 2013, and Observatori Ca l’Ou, Sant Marti Sesgueioles, Spain, from 2013 to 2015. The observed data are corrected for systematic errors according to the coarse de-correlation and SYSREM algorithms, so as to enhance the signal of the transit events. In order to consistently model the star spots and transits of this exoplanetary system, we develop a highly efficient tool STMT based on the analytic models of Mandel & Agol and Montalto et al. The physical parameters of HAT-P-20 are refined by homogeneously analyzing our new data, the radial velocity data, and the earlier photometric data in the literature with the Markov chain Monte Carlo technique. New radii and masses of both host star and planet are larger than those in the discovery paper due to the discrepancy of the radius among K-dwarfs between predicted values by standard stellar models and empirical calibration from observations. Through the analysis of all available mid-transit times calculated with the normal model and spotted model, we conclude that the periodic transit timing variations in these transit events revealed by employing the normal model are probably induced by spot crossing events. From the analysis of the distribution of occulted spots by HAT-P-20b, we constrain the misaligned architecture between the planetary orbit and the spin of the host star.

  8. On Application of the 3-Cornered Hat Technique to Radio Source Position Catalogs

    Science.gov (United States)

    Malkin, Z.

    2013-08-01

    So called ``3-cornered hat'' method (3CH) was originally developed for estimation of the stability of frequency standards (Gray1974). It was then applied for investigation of the noise level of various data, in particular, astronomical and geodetic time series and radio source position catalogs. However, despite this method is widely used, its application is not straightforward because it requires a reliable estimate of the correlations between series under investigation. Neglecting correlations often produces unacceptable results, like negative variances. In this work, we investigate a new possibility to estimate correlations between radio source position catalogs (RSC) obtained from VLBI observations.

  9. On the weight distribution in level one Demazure modules of sl2hat

    CERN Document Server

    Bliem, Thomas

    2010-01-01

    We compute the covariance of the weight distribution in level 1 Demazure modules of sl2hat. The crucial step is to compute the variance of the degree distribution. This also allows us to prove the weak law of large numbers for the degree and weight distribution using Chebyshev's inequality. We give two proofs of our main result, one by induction along Demazure's character formula, and one by using quantum calculus and the fact that Demazure characters are related to Macdonald and Rogers-Szego polynomials.

  10. Assessment of the Radiological Impact of the Inactive Uranium-Mill Tailings at Mexican Hat, Utah

    OpenAIRE

    Haywood, F. F.; Goldsmith, W. A.; Ellis, B. S.; Hubbard Jr., H. M.; Fox, W. F.; Shinpaugh, W. H.; Oak Ridge National Laboratory

    1980-01-01

    High surface soil concentrations of 226Ra and high above-ground measurements of gamma-ray intensity in the vicinity of the inactive uranium-mill tailings at Mexican Hat show both wind and water erosion of the tailings. The former mill area, occupied by a trade school at the time of this survey, shows a comparatively high level of contamination, probably from unprocessed ore on the surface of the ore storage area near the location of the former mill buildings. However, the estimated health e...

  11. Spherical "Top-Hat" Collapse in a Modified Chaplygin Gas Dominated Universe

    CERN Document Server

    Karbasi, S

    2015-01-01

    Considering perturbation growth in spherical Top-Hat model of structure formation in a generalized Chaplygin gas dominated universe, we want to study this scenario with modified Chaplygin gas model. Different parameters of this scenario for positive and negative values of A are computed. The evolution of background and collapsed region parameters are found for different cases. The stability of the model and the collapse time rate are considered in different cases. The turn-around redshifts for different values of alpha are computed; the results are in relatively good agreement with current observational data.

  12. Research on the Procedure for Computer-Aided Pattern Design of Uygur's Flowered Hats

    OpenAIRE

    Shuxian Liu; Xiaohua Li

    2013-01-01

    Xinjiang Uygur’s flowered hats is famous all over the world, it leads the world of headwear of variou nationalities within China by the variety of styles, the various ways of how to emroider pattern design and exquisite manufacture. It is unusual even in national costumes art of the world. The poupose of this study is to analyze Uygur's headwear, study the rules of Uygur's headwear pattern, analyze the characteristic of the basic pattern, research on the procedure for computer-aided pattern d...

  13. Inhibition of different histone acetyltransferases (HATs) uncovers transcription-dependent and -independent acetylation-mediated mechanisms in memory formation.

    Science.gov (United States)

    Merschbaecher, Katja; Hatko, Lucyna; Folz, Jennifer; Mueller, Uli

    2016-02-01

    Acetylation of histones changes the efficiency of the transcription processes and thus contributes to the formation of long-term memory (LTM). In our comparative study, we used two inhibitors to characterize the contribution of different histone acetyl transferases (HATs) to appetitive associative learning in the honeybee. For one we applied garcinol, an inhibitor of the HATs of the p300 (EP300 binding protein)/CBP (CREB-binding protein) family, and the HATs of the PCAF (p300/CBP-associated factor) family. As comparative agent we applied C646, a specific inhibitor that selectively blocks HATS of the p300/CBP family. Immunochemical analysis reveals differences in histone H3 acetylation in the honeybee brain, in response to the injection of either C646 or garcinol. Behavioral assessment reveals that the two drugs cause memory impairment of different nature when injected after associative conditioning: processes disturbed by garcinol are annihilated by the established transcription blocker actinomycin D and thus seem to require transcription processes. Actions of C646 are unaltered by actinomycin D, and thus seem to be independent of transcription. The outcome of our different approaches as summarized suggests that distinct HATs contribute to different acetylation-mediated processes in memory formation. We further deduce that the acetylation-mediated processes in memory formation comprise transcription-dependent and transcription-independent mechanisms.

  14. The Transit Light Curve Project. VII. The Not-So-Bloated Exoplanet HAT-P-1b

    CERN Document Server

    Winn, Joshua N; Bakos, Gaspar A; Pal, Andras; Johnson, John Asher; Williams, Peter K G; Shporer, Avi; Mazeh, Tsevi; Fernandez, Jose; Latham, David W

    2007-01-01

    We present photometry of the G0 star HAT-P-1 during six transits of its close-in giant planet, and we refine the estimates of the system parameters. Relative to Jupiter's properties, HAT-P-1b is 1.20 +/- 0.05 times larger and its surface gravity is 2.7 +/- 0.2 times weaker. Although it remains the case that HAT-P-1b is among the least dense of the known sample of transiting exoplanets, its properties are in accord with previously published models of strongly irradiated, coreless, solar-composition giant planets. The times of the transits have a typical accuracy of 1 min and do not depart significantly from a constant period.

  15. Evidence for Water in the Atmosphere of HAT-P-26b Using LDSS-3C

    CERN Document Server

    Stevenson, Kevin B; Seifahrt, Andreas; Gilbert, Greg; Line, Michael R; Desert, Jean-Michel; Fortney, Jonathan J

    2015-01-01

    The characterization of a physically-diverse set of transiting exoplanets is an important and necessary step towards establishing the physical properties linked to the production of obscuring clouds or hazes. Only planets with identifiable spectroscopic features can effectively enhance our understanding of atmospheric chemistry and metallicity. Using data acquired by the newly-commissioned LDSS-3C instrument on Magellan and the Spitzer Space Telescope, we find evidence for water in the transmission spectrum of the Neptune-mass planet HAT-P-26b. Surprisingly, we detect no trace of potassium. Our measured spectrum is best explained by either a high-metallicity, cloud-free atmosphere or a solar-metallicity atmosphere with a cloud deck at ~10 mbar. The presence of strong spectral features in our data suggests that future observations at higher precision could break this degeneracy and reveal the planet's atmospheric composition. We also update HAT-P-26b's transit ephemeris, t_0 = 2455304.65218(25) BJD_TDB, and or...

  16. Photometric observation of HAT-P-16b in the near-UV

    CERN Document Server

    Pearson, Kyle A; Sagan, Thomas A G

    2014-01-01

    We present the first primary transit light curve of the hot Jupiter HAT-P-16b in the near-UV photometric band. We observed this object on December 29,2012 in order to update the transit ephemeris, constrain its planetary parameters and search for magnetic field interference. Vidotto et al. (2011a) postulate that the magnetic field of HAT-P-16b can be constrained if its near-UV light curve shows an early ingress compared to its optical light curve, while its egress remains unchanged. However, we did not detect an early ingress in our night of observing when using a cadence of 60 seconds and an average photometric precision of 2.26mmag. We find a near-UV planetary radius of Rp=1.274+-0.057RJup which is consistent with its near-IR radius of Rp=1.289+-0.066RJup (Buchhave et al., 2010). We developed an automated reduction pipeline and modeling package to process our data. The data reduction package synthesizes a set of IRAF scripts to calibrate images and perform aperture photometry. The modeling package utilizes ...

  17. Transmission spectroscopy of the inflated exo-Saturn HAT-P-19b

    CERN Document Server

    Mallonn, M; Weingrill, J; Strassmeier, K G; Ribas, I; Carroll, T A; Herrero, E; Granzer, T; Claret, A; Schwope, A

    2015-01-01

    We observed the Saturn-mass and Jupiter-sized exoplanet HAT-P-19b to refine its transit parameters and ephemeris as well as to shed first light on its transmission spectrum. We monitored the host star over one year to quantify its flux variability and to correct the transmission spectrum for a slope caused by starspots. A transit of HAT-P-19b was observed spectroscopically with OSIRIS at the Gran Telescopio Canarias in January 2012. The spectra of the target and the comparison star covered the wavelength range from 5600 to 7600 AA. One high-precision differential light curve was created by integrating the entire spectral flux. This white-light curve was used to derive absolute transit parameters. Furthermore, a set of light curves over wavelength was formed by a flux integration in 41 wavelength channels of 50 AA width. We analyzed these spectral light curves for chromatic variations of transit depth. The transit fit of the combined white-light curve yields a refined value of the planet-to-star radius ratio o...

  18. A Mexican Hat with holes: calculating low resolution power spectra from data with gaps

    CERN Document Server

    Arevalo, P; Zhuravleva, I; Hernandez-Monteagudo, C; Revnivtsev, M

    2012-01-01

    A simple method for calculating a low-resolution power spectrum from data with gaps is described. The method is a modification of the $\\Delta$-variance method previously described by Stutzki and Ossenkopf. A Mexican Hat filter is used to single out fluctuations at a given spatial scale and the variance of the convolved image is calculated. The gaps in the image, defined by the mask, are corrected for by representing the Mexican Hat filter as a difference between two Gaussian filters with slightly different widths, convolving the image and mask with these filters and dividing the results before calculating the final filtered image. This method cleanly compensates for data gaps even if these have complicated shapes and cover a significant fraction of the data. The method was developed to deal with problematic 2D images, where irregular detector edges and masking of contaminating sources compromise the power spectrum estimates, but it can also be straightforwardly applied to 1D timing analysis or 3D data cubes f...

  19. HATS-3b: An inflated hot Jupiter transiting an F-type star

    CERN Document Server

    Bayliss, D; Penev, K; Bakos, G; Hartman, J; Jordán, A; Mancini, L; Mohler, M; Suc, V; Rabus, M; Béky, B; Csubry, Z; Buchhave, L; Henning, T; Nikolov, N; Csák, B; Brahm, R; Espinoza, N; Noyes, R; Schmidt, B; Conroy, P; Wright, D; Tinney, C; Addison, B; Sackett, P; Sasselov, D; Lázár, J; Papp, I; Sári, P

    2013-01-01

    We report the discovery by the HATSouth survey of HATS-3b, a transiting extrasolar planet orbiting a V=12.4 F-dwarf star. HATS-3b has a period of P = 3.5479d, mass of Mp = 1.07MJ, and radius of Rp = 1.38RJ. Given the radius of the planet, the brightness of the host star, and the stellar rotational velocity (vsini = 9.0km/s), this system will make an interesting target for future observations to measure the Rossiter-McLaughlin effect and determine its spin-orbit alignment. We detail the low/medium-resolution reconnaissance spectroscopy that we are now using to deal with large numbers of transiting planet candidates produced by the HATSouth survey. We show that this important step in discovering planets produces logg and Teff parameters at a precision suitable for efficient candidate vetting, as well as efficiently identifying stellar mass eclipsing binaries with radial velocity semi-amplitudes as low as 1 km/s.

  20. A Bayesian analysis of HAT-P-7b using the EXONEST algorithm

    Energy Technology Data Exchange (ETDEWEB)

    Placek, Ben [Department of Physics, University at Albany (SUNY), Albany NY (United States); Knuth, Kevin H. [Department of Physics, University at Albany (SUNY), Albany NY, USA and Department of Informatics, University at Albany (SUNY), Albany NY (United States)

    2015-01-13

    The study of exoplanets (planets orbiting other stars) is revolutionizing the way we view our universe. High-precision photometric data provided by the Kepler Space Telescope (Kepler) enables not only the detection of such planets, but also their characterization. This presents a unique opportunity to apply Bayesian methods to better characterize the multitude of previously confirmed exoplanets. This paper focuses on applying the EXONEST algorithm to characterize the transiting short-period-hot-Jupiter, HAT-P-7b (also referred to as Kepler-2b). EXONEST evaluates a suite of exoplanet photometric models by applying Bayesian Model Selection, which is implemented with the MultiNest algorithm. These models take into account planetary effects, such as reflected light and thermal emissions, as well as the effect of the planetary motion on the host star, such as Doppler beaming, or boosting, of light from the reflex motion of the host star, and photometric variations due to the planet-induced ellipsoidal shape of the host star. By calculating model evidences, one can determine which model best describes the observed data, thus identifying which effects dominate the planetary system. Presented are parameter estimates and model evidences for HAT-P-7b.

  1. A Bayesian analysis of HAT-P-7b using the EXONEST algorithm

    Science.gov (United States)

    Placek, Ben; Knuth, Kevin H.

    2015-01-01

    The study of exoplanets (planets orbiting other stars) is revolutionizing the way we view our universe. High-precision photometric data provided by the Kepler Space Telescope (Kepler) enables not only the detection of such planets, but also their characterization. This presents a unique opportunity to apply Bayesian methods to better characterize the multitude of previously confirmed exoplanets. This paper focuses on applying the EXONEST algorithm to characterize the transiting short-period-hot-Jupiter, HAT-P-7b (also referred to as Kepler-2b). EXONEST evaluates a suite of exoplanet photometric models by applying Bayesian Model Selection, which is implemented with the MultiNest algorithm. These models take into account planetary effects, such as reflected light and thermal emissions, as well as the effect of the planetary motion on the host star, such as Doppler beaming, or boosting, of light from the reflex motion of the host star, and photometric variations due to the planet-induced ellipsoidal shape of the host star. By calculating model evidences, one can determine which model best describes the observed data, thus identifying which effects dominate the planetary system. Presented are parameter estimates and model evidences for HAT-P-7b.

  2. Magic hat economics: counter-cultural ideals and practices of the Nordic Ting community

    Directory of Open Access Journals (Sweden)

    Janne Juhana Rantala

    2009-01-01

    Full Text Available The author's anthropological study concerns one of today’s communities with no shared belief system, but with a clear spiritualist orientation. The Nordic Ting Community does not have any defined or committing roles, specialized distribution of tasks, entrance fee to their two annual gatherings, membership or any formal hierarchy. This exiguity of structural differentiation could well be understood to represent ‘subjective spirituality’. This thesis refers to the decline of institutional forms of religion with, instead, an increase in subjective experience in spirituality. This presentation shows that at least in the author's field of study, there hardly exists any increase in emphasis on individualism in spirituality. Instead the material indicates a relatively long continuum of a self-organized type of communality which could be understood as neither individualistic nor collectivistic. The type of agency observed in the social action of the studied network-like field is intersubjective. This article focuses on the use of the magic hat and the combination of ideals and practices characteristic to the Ting Community, which the author calls the magic hat economics. It is argued that by looking at these kinds of intermediating objects, the problem of individualization can be seen much more clearly

  3. The nature of the Napoleon's Hat nebula of SN 1987A

    Science.gov (United States)

    Wang, L.; Dyson, J. E.; Kahn, F. D.

    1993-03-01

    The interstellar and circumstellar environment of SN 1987A is modeled. The geometries of Napoleon's Hat nebula and the dark bay suggest that there is relative motion between the SN progenitor and the surrounding ISM. Most of the dark bay can be identified with the bubble produced by the fast blue supergiant (BSG) wind before the red supergiant (RSG) stage. The relative motion between the star and the ISM is the primary reason for the star being at the edge of this bubble. After the first BSG stage, the star evolves to the RSG stage; the wind velocity is now typically 10 km/s and the mass loss rate is around 10 exp -5 solar mass/yr. The star eventually breaks through the bubble produced in the early BSG stage and starts to interact directly with the ISM outside, thus producing Napoleon's Hat. The success of this model is convincing proof of the BSG-RSG-BSG evolutionary model for the SN 1987A progenitors, and shows that is has moved from the site where it was formed.

  4. Infrared Secondary Eclipse Photometry of the Transiting Exoplanet HAT-P-1b

    Science.gov (United States)

    Todorov, Kamen; Deming, D.; Harrington, J.; Stevenson, K.; Bowman, W.; Nymeyer, S.; Fortney, J.; Bakos, G.

    2009-09-01

    We examine the secondary eclipse of the exoplanet HAT-P-1b using the IRAC instrument on board the Spitzer Space Telescope. This planet is close to the boundary between the pM and pL classes of hot Jupiters, which makes it an important test case for theoretical models of temperature inversions in the atmospheres of this type of gas giants. Our analysis shows secondary eclipse depths for HAT-P-1b, as a fraction of the stellar flux, to be: 0.075% +/- 0.008% (3.6 micron), 0.128% +/- 0.022% (4.5 micron), 0.183% +/- 0.031% (5.8 micron) and 0.230% +/- 0.034% (8.0 micron). These values can be produced, within errors, by a 1500K black body, or alternatively by a hot Jupiter with a small temperature inversion in its atmosphere. In both cases, the planet must radiate a significant amount of the energy received from the star through its day side, which suggests that little energy is redistributed through atmospheric circulation to be emitted through the night side of the planet. It has been suggested that this planet is inflated through tidal dissipation, based on its large radius and non-zero eccentricity allowed by the radial velocity data. By timing the secondary transit, we are able to determine that the orbit is very close to circular. The leading author was funded by the NASA Astrobiology Institute SUIA program.

  5. Kinetic features revealed by top-hat electrostatic analysers: numerical simulations and instrument response results

    Science.gov (United States)

    De Marco, Rossana; Marcucci, Maria Federica; Brienza, Daniele; Bruno, Roberto; Consolini, Giuseppe; Perrone, Denise; Valentini, Franceso; Servidio, Sergio; Stabile, Sara; Pezzi, Oreste; Sorriso-Valvo, Luca; Lavraud, Benoit; De Keyser, Johan; Retinò, Alessandro; Fazakerley, Andrew; Wicks, Robert; Vaivads, Andris; Salatti, Mario; Veltri, Pierliugi

    2017-04-01

    Turbulence Heating ObserveR (THOR) is the first mission devoted to study energization, acceleration and heating of turbulent space plasmas, and designed to perform field and particle measurements at kinetic scales in different near-Earth regions and in the solar wind. Solar Orbiter (SolO), together with Solar Probe Plus, will provide the first comprehensive remote and in situ measurements which are critical to establish the fundamental physical links between the Sun's dynamic atmosphere and the turbulent solar wind. The fundamental process of turbulent dissipation is mediated by physical mechanism that occur at a variety of temporal and spatial scales, and most efficiently at the kinetics scales. Hybrid Vlasov-Maxwell simulations of solar-wind turbulence show that kinetic effects manifest as particle beams, production of temperature anisotropies and ring-like modulations, preferential heating of heavy ions. We use a numerical code able to reproduce the response of a typical electrostatic analyzer of top-hat type starting from velocity distribution functions (VDFs) generated by Hybrid Vlasov-Maxwell (HVM) numerical simulations. Here, we show how optimized particle measurements by top-hat analysers can capture the kinetic features injected by turbulence in the VDFs.

  6. Design and Optimization of a Compact Wideband Hat-Fed Reflector Antenna for Satellite Communications

    Science.gov (United States)

    Geterud, Erik G.; Yang, Jian; Ostling, Tomas; Bergmark, Pontus

    2013-01-01

    We present a new design of the hat-fed reflector antenna for satellite communications, where a low reflection coefficient, high gain, low sidelobes and low cross-polar level are required over a wide frequency band. The hat feed has been optimized by using the Genetic Algorithm through a commercial FDTD solver, QuickWave-V2D, together with an own developed optimization code. The Gaussian vertex plate has been applied at the center of the reflector in order to improve the reflection coefficient and reduce the far-out sidelobes. A parabolic reflector with a ring-shaped focus has been designed for obtaining nearly 100% phase efficiency. The antenna's reflection coefficient is below -17 dB and the radiation patterns satisfy the M-x standard co- and cross-polar sidelobe envelopes for satellite ground stations over a bandwidth of 30%. A low-cost monolayer radome has been designed for the antenna with satisfactory performance. The simulations have been verified by measurements; both of them are presented in the paper.

  7. Implications of the Secondary Eclipse of Exoplanet HAT-P-11b

    Science.gov (United States)

    Barry, Richard K.; Deming, L. D.; Bakos, G.; Harrington, J.; Madhusudhan, N.; Noyes, R.; Seager, S.

    2010-01-01

    We observed exoplanet HAT-P-11b and have successfully detected its secondary eclipse. We conducted observations using the Spitzer Space Telescope in the post-cryo mission at 3.6 microns for a period of 22 hours centered on the anticipated secondary eclipse time, to detect the eclipse and determine its phase. Having detected the secondary eclipse, we are at present making a more focused series of observations in both the 3.6 and 4.5 micron bands to fully characterize it. HAT-P-11b is one of only two known exo-Neptunes and has a period of 4.8878 days, radius of 0.422 RJ, mass of 0.081 MJ and semi-major axis 0.053 AU. Measurements of the secondary eclipse will serve to clarify two key issues; 1) the planetary brightness temperature and the nature of its atmosphere, and 2) the eccentricity of its orbit, with implications for its dynamical evolution. We discuss implications of these observations.

  8. HATS-3b: AN INFLATED HOT JUPITER TRANSITING AN F-TYPE STAR

    Energy Technology Data Exchange (ETDEWEB)

    Bayliss, D.; Zhou, G.; Schmidt, B. [Research School of Astronomy and Astrophysics, Australian National University, Canberra, ACT 2611 (Australia); Penev, K.; Bakos, G. Á.; Hartman, J. D.; Csubry, Z. [Department of Astrophysical Sciences, Princeton University, NJ 08544 (United States); Jordán, A.; Suc, V.; Rabus, M.; Brahm, R.; Espinoza, N. [Departamento de Astronomía y Astrofísica, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, 7820436 Macul, Santiago (Chile); Mancini, L.; Mohler-Fischer, M.; Henning, T.; Nikolov, N.; Csák, B. [Max Planck Institute for Astronomy, Heidelberg (Germany); Béky, B.; Noyes, R. W. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA (United States); Buchhave, L., E-mail: daniel@mso.anu.edu.au [Niels Bohr Institute, Copenhagen University (Denmark); and others

    2013-11-01

    We report the discovery by the HATSouth survey of HATS-3b, a transiting extrasolar planet orbiting a V = 12.4 F dwarf star. HATS-3b has a period of P = 3.5479 days, mass of M{sub p} = 1.07 M {sub J}, and radius of R{sub p} = 1.38 R {sub J}. Given the radius of the planet, the brightness of the host star, and the stellar rotational velocity (vsin i = 9.0 km s{sup –1}), this system will make an interesting target for future observations to measure the Rossiter-McLaughlin effect and determine its spin-orbit alignment. We detail the low-/medium-resolution reconnaissance spectroscopy that we are now using to deal with large numbers of transiting planet candidates produced by the HATSouth survey. We show that this important step in discovering planets produces log g and T {sub eff} parameters at a precision suitable for efficient candidate vetting, as well as efficiently identifying stellar mass eclipsing binaries with radial velocity semi-amplitudes as low as 1 km s{sup –1}.

  9. Transmission Spectroscopy of HAT-P-32Ab with GTC/OSIRIS

    Science.gov (United States)

    Nortmann, Lisa; Pallé, Enric; Murgas, Felipe; Dreizler, Stefan; Iro, Nicolas; Cabrera-Lavers, Antonio

    2015-12-01

    I will present one of the latest results of our GTC exoplanet transit spectroscopy survey. Over the last years our group has obtained ground-based optical (538 nm - 918 nm) spectrophotometric transit observations for several hot Jupiters including HAT-P-32Ab using the OSIRIS (Optical System for Imaging and low Resolution Integrated Spectroscopy) instrument at the Spanish 10-meter telescope GTC.I will discuss the source, nature and proper correction of instrument specific systematic noise we found to affect our data. After its correction, we were able to yield high quality results with a precision between 482 and 1703 ppm depending on the wavelength channel. We measure a flat optical transmission spectrum for HAT-P-32Ab, consistent with the results of Gibson et al. (2013, MNRAS, 436, 2974) obtained with GMOS at Gemini-North. This independent reproduction of consistent results re-establishes faith in the reliability of ground-based transmission spectroscopy and emphasizes the high potential of OSIRIS at the GTC as a tool to complement current and future space-based observations.

  10. The G-HAT Search for Advanced Extraterrestrial Civilizations: The Reddest Extended WISE Sources

    Science.gov (United States)

    Maldonado, Jessica; Povich, Matthew S.; Wright, Jason; Griffith, Roger; Sigurdsson, Steinn; Mullan, Brendan L.

    2015-01-01

    Freeman Dyson (1960) theorized how to identify possible signatures of advanced extra-terrestrial civilizations by their waste heat, an inevitable byproduct of a civilization using a significant fraction of the luminosity from their host star. If a civilizations could tap the starlight throughout their host galaxy their waste heat would be easily detectable by recent infrared surveys. The Glimpsing Heat from Alien Technologies (G-HAT) pilot project aims to place limits on the existence of extraterrestrial civilizations at pan-galactic scales. We present results from the G-HAT cleaned catalog of 563 extremely red, extended high Galactic latitude (|b| ≥ 10) sources from the WISE All-Sky Catalog. Our catalog includes sources new to the scientific literature along with well-studied objects (e.g. starburst galaxies, AGN, and planetary nebulae) that exemplify extreme WISE colors. Objects of particular interest include a supergiant Be star (48 Librae) surrounded by a resolved, mid-infrared nebula, possibly indicating dust in the stellar wind ejecta, and a curious cluster of seven extremely red WISE sources (associated with IRAS 04287+6444) that have no optical counterparts.

  11. 亲历“黑客帝国”——2008 Black Hat USA大会纪实

    Institute of Scientific and Technical Information of China (English)

    许舟平

    2008-01-01

    2008年8月6日,第12届Black Hat USA大会在美国内华达州拉斯维加斯揭开了帷幕。在为期2天的Black Hat Briefings会议中,共有来自全球的超过4500名参会者来到酷热的拉斯维加斯,这些人囊括了全世界最著名的软件安全厂商、独立安全研究人员和众多的黑客。

  12. Chaos in classical string dynamics in $\\hat{\\gamma}$ deformed $AdS_5 \\times T^{1,1}$

    CERN Document Server

    Panigrahi, Kamal L

    2016-01-01

    We consider a circular string in $\\hat{\\gamma}$ deformed $AdS_5 \\times T^{1,1}$ which is localized in the center of $AdS_5$ and winds around the two circles of deformed $T^{1,1}$. We observe chaos in the phase space of the circular string implying non-integrability of string dynamics. The chaotic behaviour in phase space is controlled by energy as well as the deforming parameter $\\hat{\\gamma}$. We further show that the point like object exhibits non-chaotic behaviour. Finally we calculate the Lyapunov exponent for both extended and point like object in support of our first result.

  13. Top-hat shaped corneal trephination for penetrating keratoplasty using the femtosecond laser: a histomorphological study.

    Science.gov (United States)

    Kook, Daniel; Derhartunian, Victor; Bug, Reinhold; Kohnen, Thomas

    2009-08-01

    To evaluate a novel technique for penetrating keratoplasty (PK) with the use of a new software algorithm for the femtosecond laser, designed to create penetrating cuts in a top hat configuration. Consecutive histomorphological case series. Twelve eyes of 12 patients underwent penetrating keratoplasty by means of a 60-kHz femtosecond laser (IntraLase, Irvine, California) with a software specifically developed for corneal surgery. Of the 12 patients, the reason for keratoplasty was keratoconus in 4 patients, bullous keratopathy in 6 patients, keratotorus in 1 patient, and status post chemical burn in 1 patient. A new software was used to create penetrating cuts in a top-hat-shaped configuration. In all cases, cutting parameters were identical in all donor and corresponding host corneas: 7.0 mm diameter of the anterior side cut, 8.5-8.7 mm diameter of the posterior side cut, and a depth of 300 microm for the lamellar cut. In all cases, a complete penetrating cut with the laser in the host cornea was not intended intraoperatively. Complete penetration was performed subsequently and manually with a diamond knife because of logistic conditions. Trephined corneoscleral rings and button corneas were analyzed macroscopically and histologically to determine cut quality. All procedures were performed without any complications. With application of appropriate combinations of pulse energy and spacing, trephination took less than 200 seconds. Macroscopic examination and histology of donor and recipient specimens showed a straight, smooth cut with perpendicular edges in all donor buttons. No corneal edema and no visible damage to the keratocyte nuclei were found. At the region of manual dissection, a small stromal tissue-tag was present in parts of the circumference in all donor buttons. No evidence of any cut complication was noted. Top-hat-shaped penetrating keratoplasty using the IntraLase femtosecond laser enables a quick and sufficient trephination of both human donor and

  14. Estimation of gas-particle partitioning coefficients (Kp) of carcinogenic polycyclic aromatic hydrocarbons in carbonaceous aerosols collected at Chiang-Mai, Bangkok and Hat-Yai, Thailand.

    Science.gov (United States)

    Pongpiachan, Siwatt; Ho, Kin Fai; Cao, Junji

    2013-01-01

    To assess environmental contamination with carcinogens, carbonaceous compounds, water-soluble ionic species and trace gaseous species were identified and quantified every three hours for three days at three different atmospheric layers at the heart of Chiang-Mai, Bangkok and Hat-Yai from December 2006 to February 2007. A DRI Model 2001 Thermal/Optical Carbon Analyzer with the IMPROVE thermal/optical reflectance (TOR) protocol was used to quantify the organic carbon (OC) and elemental carbon (EC) contents in PM10. Diurnal and vertical variability was also carefully investigated. In general, OC and EC mass concentration showed the highest values at the monitoring period of 21.00-00.00 as consequences of human activities at night bazaar coupled with reduction of mixing layer, decreased wind speed and termination of photolysis at nighttime. Morning peaks of carbonaceous compounds were observed during the sampling period of 06:00-09:00, emphasizing the main contribution of traffic emission in the three cities. The estimation of incremental lifetime particulate matter exposure (ILPE) raises concern of high risk of carbonaceous accumulation over workers and residents living close to the observatory sites. The average values of incremental lifetime particulate matter exposure (ILPE) of total carbon at Baiyoke Suit Hotel and Baiyoke Sky Hotel are approximately ten times higher than those air samples collected at Prince of Songkla University Hat-Yai campus corpse incinerator and fish-can manufacturing factory but only slightly higher than those of rice straw burning in Songkla province. This indicates a high risk of developing lung cancer and other respiratory diseases across workers and residents living in high buildings located in Pratunam area. Using knowledge of carbonaceous fractions in PM10, one can estimate the gas-particle partitioning of polycyclic aromatic hydrocarbons (PAHs). Dachs-Eisenreich model highlights the crucial role of adsorption in gas

  15. Inhibition of Different Histone Acetyltransferases (HATs) Uncovers Transcription-Dependent and -Independent Acetylation-Mediated Mechanisms in Memory Formation

    Science.gov (United States)

    Merschbaecher, Katja; Hatko, Lucyna; Folz, Jennifer; Mueller, Uli

    2016-01-01

    Acetylation of histones changes the efficiency of the transcription processes and thus contributes to the formation of long-term memory (LTM). In our comparative study, we used two inhibitors to characterize the contribution of different histone acetyl transferases (HATs) to appetitive associative learning in the honeybee. For one we applied…

  16. Partition functions of 3d $\\hat D$-quivers and their mirror duals from 1d free fermions

    CERN Document Server

    Assel, Benjamin; Felix, Jan

    2015-01-01

    We study the matrix models calculating the sphere partition functions of 3d gauge theories with $\\mathcal{N}=4$ supersymmetry and a quiver structure of a $\\hat D$ Dynkin diagram (where each node is a unitary gauge group). As in the case of necklace ($\\hat A $) quivers, we can map the problem to that of free fermion quantum mechanics whose complicated Hamiltonian we find explicitly. Many of these theories are conjectured to be dual under mirror symmetry to certain unitary linear quivers with extra Sp nodes or antisymmetric hypermultiplets. We show that the free fermion formulations of such mirror pairs are related by a linear symplectic transformation. We then study the large N expansion of the partition function, which as in the case of the $\\hat A$-quivers is given to all orders in 1/N by an Airy function. We simplify the algorithm to calculate the numerical coefficients appearing in the Airy function and evaluate them for a wide class of $\\hat D$-quiver theories.

  17. Diagonalization of transfer matrix of supersymmetry $u_q(\\hat{sl}(m+1|n+1))$ chain with a boundary

    CERN Document Server

    Kojima, Takeo

    2012-01-01

    We study the supersymmetry $U_q(\\hat{sl}(M+1|N+1))$ analogue of the supersymmetric t-J model with a boundary, in the framework of the algebraic analysis method. We diagonalize the commuting transfer matrix by using the bosonization of the vertex operator associated with the quantum affine supersymmetry.

  18. Energy level alignment at the interface of NPB/HAT-CN/graphene for flexible organic light-emitting diodes

    Science.gov (United States)

    Oh, Eonseok; Park, Soohyung; Jeong, Junkyeong; Kang, Seong Jun; Lee, Hyunbok; Yi, Yeonjin

    2017-01-01

    Graphene is highly promising as an electrode for flexible optoelectronic devices due to its excellent conductivity and transparency. However, it is necessary to tailor its work function with a charge injection layer in order to obtain favorable energy level alignment for efficient charge injection. An adequate charge injection layer can only be chosen with the understanding of the interfacial electronic structure between a charge transport layer and an electrode. In this study, we investigated the energy level alignment of N,N‧-diphenyl-1,1‧-biphenyl-4,4‧-diamine (NPB)/hexaazatriphenylene hexacarbonitrile (HAT-CN)/graphene using in situ ultraviolet and X-ray photoelectron spectroscopy measurements. The effective work function of graphene was significantly increased by 0.94 eV by the HAT-CN hole injection layer (HIL) due to the interface dipole formation. In addition, the charge generation barrier (CGB) between NPB and HAT-CN, which plays a decisive role in charge injection efficiency with a charge generation HIL, was measured to be 0.66 eV. This CGB on graphene is the same as the CGBs on other electrodes, and smaller than that of the widely-used MoO3 HIL. Therefore, HAT-CN could be a promising HIL for efficient flexible organic light-emitting diodes with a graphene anode.

  19. BALTIMORE’S M.S. LEVY AND SONS: Straw Hat Makers to the World, 1870-1960

    Directory of Open Access Journals (Sweden)

    Jessica I. Elfenbein

    2008-01-01

    Full Text Available From 1860 to 1960, Baltimore was an important center of the men’s ready-to- wear clothing industries. German-speaking Jews created many businesses that manufactured and sold underwear, men’s suits, and specialty items like hats and umbrellas. The straw hat making firm started by M.S. Levy is an interesting example of a Baltimore-based, family-owned niche business that existedfor 90 years, through three generations ofa singlefamily. The M.S. Levy and Sons story has two main chapters. The first covers the period from its founding by Michael Simon Levy in the 1870s through his son Julius’ death in 1926. This is the story of ascension. A premonition about the coming popularity of straw hats, together with a small but important technological breakthrough, positioned a family and a small industry for significant expansion. The second period began in 1930 with the introduction of wool felt hats and the death of William, M.S. Levy’s oldest son, and includes the Great Depression, unionization, merger, third generation leadership, and finally, in 1959, the family’s withdrawal from the industry. This later period, as told by grandsons of the founder, is the story of struggle followed by decline.

  20. Next-to-Leading Order Jet Physics with BlackHat

    CERN Document Server

    Berger, C F; Dixon, L J; Cordero, F Febres; Forde, D; Gleisberg, T; Ita, H; Kosower, D A; Maitre, D

    2009-01-01

    We present several results obtained using the BlackHat next-to-leading order QCD program library, in conjunction with SHERPA. In particular, we present distributions for vector boson plus 1,2,3-jet production at the Tevatron and at the asymptotic running energy of the Large Hadron Collider, including new Z+3-jet distributions. The Z+2-jet predictions for the second-jet P_T distribution are compared to CDF data. We present the jet-emission probability at NLO in W+2-jet events at the LHC, where the tagging jets are taken to be the ones furthest apart in pseudorapidity. We analyze further the large left-handed W polarization, identified in our previous study, for W bosons produced at high P_T at the LHC.

  1. Rayleigh scattering in the transmission spectrum of HAT-P-18b

    CERN Document Server

    Kirk, J; Louden, T; Doyle, A P; Skillen, I; McCormac, J; Irwin, P G J; Karjalainen, R

    2016-01-01

    We have performed low-resolution ground-based transmission spectroscopy of the hot Jupiter HAT-P-18b using the ACAM instrument on the William Herschel Telescope (WHT). We detect a bluewards slope extending across our optical transmission spectrum which runs from 4750 Ang to 9250 Ang. The slope is consistent with Rayleigh scattering at the equilibrium temperature of the planet. We do not detect enhanced sodium absorption, which indicates a high altitude haze is masking the feature and giving rise to the Rayleigh slope. Our detection of an opacity source within a hot Jupiter atmosphere demonstrates that ground-based observations can provide transmission spectra with precision comparable to the Hubble Space Telescope (HST).

  2. A Near-Infrared Transmission Spectrum for the Warm Saturn HAT-P-12b

    CERN Document Server

    Line, Michael R; Deming, Drake; Wilkins, Ashlee; Desert, Jean-Michel

    2013-01-01

    We present a HST WFC3 transmission spectrum for the transiting exoplanet HAT-P-12b. This warm (1000 K) sub-Saturn-mass planet has a smaller mass and a lower temperature than the hot-Jupiters that have been studied so far. We find that the planet's measured transmission spectrum lacks the expected water absorption feature for a hydrogen-dominated atmosphere, and is instead best-described by a model with high-altitude clouds. Using a frequentist hypothesis testing procedure, we can rule out a hydrogen-dominated cloud free atmosphere to 4.9$\\sigma$. When combined with other recent WFC3 studies, our observations suggest that clouds may be common in exo-planetary atmospheres.

  3. 3D spin-orbit angle of Kepler-25 and HAT-P-7

    Directory of Open Access Journals (Sweden)

    Benomar Othman

    2015-01-01

    Full Text Available The number of discovered exoplanets now exceeds 1500, mostly due to the Kepler space instrument observations. Many of these planet orbit in less than a week around their host stars. This implies that the inward migration of those planets is a basic ingredient of successful theories of planet formation and evolution. Several mechanisms have been proposed to explain the observed periods, which lead to different orbit eccentricity and obliquity distributions. Here we summarise and discuss the results of obliquities for two Kepler stars: HAT-P-7 and Kepler-25. These are interesting stellar systems as we could carry out a joint analysis using asteroseismology, transit lightcurve and the Rossiter-McLaughlin effect in order to measure the three dimensional obliquity.

  4. Self-assembled ordered structures in thin films of HAT5 discotic liquid crystal

    Directory of Open Access Journals (Sweden)

    Piero Morales

    2010-05-01

    Full Text Available Thin films of the discotic liquid crystal hexapentyloxytriphenylene (HAT5, prepared from solution via casting or spin-coating, were investigated by atomic force microscopy and polarizing optical microscopy, revealing large-scale ordered structures substantially different from those typically observed in standard samples of the same material. Thin and very long fibrils of planar-aligned liquid crystal were found, possibly formed as a result of an intermediate lyotropic nematic state arising during the solvent evaporation process. Moreover, in sufficiently thin films the crystallization seems to be suppressed, extending the uniform order of the liquid crystal phase down to room temperature. This should be compared to the bulk situation, where the same material crystallizes into a polymorphic structure at 68 °C.

  5. A cohort study of workers compensated for mercury intoxication following employment in the fur hat industry.

    Science.gov (United States)

    Merler, E; Boffetta, P; Masala, G; Monechi, V; Bani, F

    1994-11-01

    This article presents the preliminary results of a follow-up study (1950-1992) of 1,146 subjects (person-years = 30,954; 23,055 for women) receiving compensation for mercury poisoning. In a province of Tuscany in central Italy, severe exposure to mercury occurred during fur hat production. A deficit in all causes of mortality was observed in both sexes, whereas mortality due to cancer was slightly higher than expected. Mortality from stomach cancer was significantly elevated for men and women. A significant excess of lung cancer was observed in women only. Whereas the excess of stomach cancer probably reflects elevated rates in the study area rather than exposure to mercury, the excess of lung cancer mortality does appear to be related to mercury exposure. Smoking habits or other exposures at work do not seem to explain the excess of lung cancer.

  6. Successful Asteroseismology for a Better Characterization of the Exoplanet HAT-P-7b

    CERN Document Server

    Oshagh, M; Benomar, O; Dupret, M -A; Monteiro, M J P F G; Scuflaire, R; Santos, N C

    2013-01-01

    It is well known that asteroseismology is the unique technique permitting the study of the internal structure of pulsating stars using their pulsational frequencies, which is per se very important. It acquires an additional value when the star turns out to be a planet host. In this case, the asteroseismic study output may be a very important input for the study of the planetary system. With this in mind, we use the large time-span of the Kepler public data obtained for the star system HAT-P-7, first to perform an asteroseismic study of the pulsating star using Time-Dependent-Convection (TDC) models. Secondly, we make a revision of the planet properties in the light of the asteroseismic study.

  7. HAT-P-55b: A Hot Jupiter Transiting a Sun-like Star

    CERN Document Server

    Juncher, D; Hartman, J D; Bakos, G Á; Bieryla, A; Kovács, T; Boisse, I; Latham, D W; Kovács, G; Bhatti, W; Csubry, Z; Penev, K; de Val-Borro, M; Falco, E; Torres, G; Noyes, R W; Lázár, J; Papp, I; Sári, P

    2015-01-01

    We report the discovery of a new transiting extrasolar planet, HAT-P-55b. The planet orbits a V = 13.207 +/- 0.039 sun-like star with a mass of 1.013 +/- 0.037 solar masses, a radius of 1.011 +/- 0.036 solar radii and a metallicity of -0.03 +/- 0.08. The planet itself is a typical hot Jupiter with a period of 3.5852467 +/- 0.0000064 days, a mass of 0.582 +/- 0.056 Jupiter masses and a radius of 1.182 +/- 0.055 Jupiter radii. This discovery adds to the increasing sample of transiting planets with measured bulk densities, which is needed to put constraints on models of planetary structure and formation theories.

  8. Wave turbulence description of interacting particles: Klein-Gordon model with a Mexican-hat potential

    CERN Document Server

    Gallet, Basile; Dubrulle, Bérengère

    2015-01-01

    In field theory, particles are waves or excitations that propagate on the fundamental state. In experiments or cosmological models one typically wants to compute the out-of-equilibrium evolution of a given initial distribution of such waves. Wave Turbulence deals with out-of-equilibrium ensembles of weakly nonlinear waves, and is therefore well-suited to address this problem. As an example, we consider the complex Klein-Gordon equation with a Mexican-hat potential. This simple equation displays two kinds of excitations around the fundamental state: massive particles and massless Goldstone bosons. The former are waves with a nonzero frequency for vanishing wavenumber, whereas the latter obey an acoustic dispersion relation. Using wave turbulence theory, we derive wave kinetic equations that govern the coupled evolution of the spectra of massive and massless waves. We first consider the thermodynamic solutions to these equations and study the wave condensation transition, which is the classical equivalent of Bo...

  9. "It's your badge of inclusion": the Red Hat Society as a gendered subculture of aging.

    Science.gov (United States)

    Barrett, Anne E; Pai, Manacy; Redmond, Rebecca

    2012-12-01

    Although studies document the health-enhancing effects of social engagement, they reveal little about the underlying mechanisms operating within specific organizational contexts. Limited attention is given to the role of inequality--particularly age and gender--in shaping either the organizations to which we belong or their consequences for our well-being. We address this issue by examining the Red Hat Society, a social organization for middle-aged and older women. Interviews with members (n=52) illustrate how age and gender inequality interact to shape the organization, which can be viewed as a gendered subculture of aging. Drawing on this framework, we discuss four processes through which participation generates benefits for older women involved in age- and gender-segregated organizations: enhancing social networks, countering invisibility, creating positive frames for aging experiences, and promoting youthful identities.

  10. Using Edward de Bono's six hats game to aid critical thinking and reflection in palliative care.

    Science.gov (United States)

    Kenny, Lesley J

    2003-03-01

    This article describes the use of a creative thinking game to stimulate critical thinking and reflection with qualified health professionals undertaking palliative care education. The importance of reflective practice in nursing is well documented and numerous models are available. However, the author as a nurse teacher has found that many of these models are either too simple or too complex to be valuable in practice. The six hats game, devised by Edward de Bono, is a method that stimulates a variety of types of thinking and when used as a means of reflection helps students to become more critical about their practice. Using this game with a palliative care case study the author demonstrates how thinking more creatively about the patients' perceived needs and problems can assist in developing reflective skills. The article concludes with a discussion on some of the challenges of using this method and suggestions for future practical uses.

  11. High-harmonic and terahertz wave spectroscopy (HATS) for aligned molecules

    CERN Document Server

    Huang, Yindong; Zhao, Jing; Wang, Xiaowei; Lü, Zhihui; Zhang, Dongwen; Yuan, Jianmin; Zhao, Zengxiu

    2016-01-01

    We present the experimental and theoretical details of our recent published letter [Phys. Rev. Lett. 115. 123002] on synchronized high-harmonic and terahertz-wave spectroscopy (HATS) from nonadiabatically aligned nitrogen molecules in dual-color laser fields. Associating the alignment-angle dependent terahertz wave generation with the synchronizing high-harmonic signal, the angular differential photoionization cross section (PICS) for molecules can be reconstructed, and the minima of the angle on PICS show great convergence between the theoretical predictions and the experimental deduced results. We also show the optimal relative phase between the dual-color laser fields for terahertz wave generation dose not change with the alignment angle at a precision of $50$ attoseconds. This all-optical method provides an alternative for investigating molecular structures and dynamics.

  12. Viscous Chaplygin Gas Models as a Spherical Top-Hat Collapsing Fluids

    CERN Document Server

    Jawad, Abdul

    2016-01-01

    We study the spherical top-hat collapse in Einstein gravity and loop quantum cosmology by taking the non-linear evolution of viscous modified variable chaplygin gas and viscous generalized cosmic chaplygin gas. We calculate the equation of state parameter, square speed of sound, perturbed equation of state parameter, perturbed square speed of sound, density contrast and divergence of peculiar velocity in perturbed region and discussed their behavior. It is observed that both chaplygin gas models support the spherical collapse in Einstein as well as loop quantum cosmology because density contrast remains positive in both cases and the perturbed equation of state parameter remains positive at the present epoch as well as near future. It is remarked here that these parameters provide the consistence results for both chaplygin gas models in both gravities.

  13. Neues in der Varizenchirurgie: Welche Bedeutung hat die Chirurgie in Zeiten von Laser, Schaum und Radiofrequenz

    Directory of Open Access Journals (Sweden)

    Assadian A

    2012-01-01

    Full Text Available Die Therapie der Stammvarikose der Vena saphena magna und parva hat in den vergangenen 10 Jahren einen Innovationsschub erlebt. Mehrere neue – mehrheitlich der Crossektomie und Stripping gegenüber als überlegen dargestellte – ausschließlich endoluminale Therapieoptionen haben den Markt erobert. Die Operation ist sicher nicht mehr für alle Patienten und alle Indikationen die optimale Therapie, die derzeitige Evidenz steht jedoch im krassen Gegensatz zur mehrheitlich aus merkantilen Absichten geführten Diskussion. Aus der derzeitigen Evidenzlage und Erfahrung sind sehr großlumige ( 10 mm, geschlängelte Stammvarizen mit ausgeprägten Seitenästen weiterhin chirurgisch besser versorgt. Auch ist ein BMI 27 als Risikofaktor für Rekanalisation nach endoluminalem Verfahren zu sehen.

  14. HAT-P-65b and HAT-P-66b: Two Transiting Inflated Hot Jupiters and Observational Evidence for the Reinflation of Close-in Giant Planets

    Science.gov (United States)

    Hartman, J. D.; Bakos, G. Á.; Bhatti, W.; Penev, K.; Bieryla, A.; Latham, D. W.; Kovács, G.; Torres, G.; Csubry, Z.; de Val-Borro, M.; Buchhave, L.; Kovács, T.; Quinn, S.; Howard, A. W.; Isaacson, H.; Fulton, B. J.; Everett, M. E.; Esquerdo, G.; Béky, B.; Szklenar, T.; Falco, E.; Santerne, A.; Boisse, I.; Hébrard, G.; Burrows, A.; Lázár, J.; Papp, I.; Sári, P.

    2016-12-01

    We present the discovery of the transiting exoplanets HAT-P-65b and HAT-P-66b, with orbital periods of 2.6055 and 2.9721 days, masses of 0.527+/- 0.083 {M}{{J}} and 0.783+/- 0.057 {M}{{J}}, and inflated radii of 1.89+/- 0.13 {R}{{J}} and {1.59}-0.10+0.16 {R}{{J}}, respectively. They orbit moderately bright (V=13.145+/- 0.029 and V=12.993+/- 0.052) stars of mass 1.212+/- 0.050 {M}⊙ and {1.255}-0.054+0.107 {M}⊙ . The stars are at the main-sequence turnoff. While it is well known that the radii of close-in giant planets are correlated with their equilibrium temperatures, whether or not the radii of planets increase in time as their hosts evolve and become more luminous is an open question. Looking at the broader sample of well-characterized close-in transiting giant planets, we find that there is a statistically significant correlation between planetary radii and the fractional ages of their host stars, with a false-alarm probability of only 0.0041%. We find that the correlation between the radii of planets and the fractional ages of their hosts is fully explained by the known correlation between planetary radii and their present-day equilibrium temperatures; however, if the zero-age main-sequence equilibrium temperature is used in place of the present-day equilibrium temperature, then a correlation with age must also be included to explain the planetary radii. This suggests that, after contracting during the pre-main-sequence, close-in giant planets are reinflated over time due to the increasing level of irradiation received from their host stars. Prior theoretical work indicates that such a dynamic response to irradiation requires a significant fraction of the incident energy to be deposited deep within the planetary interiors. Based on observations obtained with the Hungarian-made Automated Telescope Network. Based on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology

  15. HATS-3b: An Inflated Hot Jupiter Transiting an F-type Star

    Science.gov (United States)

    Bayliss, D.; Zhou, G.; Penev, K.; Bakos, G. Á.; Hartman, J. D.; Jordán, A.; Mancini, L.; Mohler-Fischer, M.; Suc, V.; Rabus, M.; Béky, B.; Csubry, Z.; Buchhave, L.; Henning, T.; Nikolov, N.; Csák, B.; Brahm, R.; Espinoza, N.; Noyes, R. W.; Schmidt, B.; Conroy, P.; Wright, D. J.; Tinney, C. G.; Addison, B. C.; Sackett, P. D.; Sasselov, D. D.; Lázár, J.; Papp, I.; Sári, P.

    2013-11-01

    We report the discovery by the HATSouth survey of HATS-3b, a transiting extrasolar planet orbiting a V = 12.4 F dwarf star. HATS-3b has a period of P = 3.5479 days, mass of Mp = 1.07 M J, and radius of Rp = 1.38 R J. Given the radius of the planet, the brightness of the host star, and the stellar rotational velocity (vsin i = 9.0 km s-1), this system will make an interesting target for future observations to measure the Rossiter-McLaughlin effect and determine its spin-orbit alignment. We detail the low-/medium-resolution reconnaissance spectroscopy that we are now using to deal with large numbers of transiting planet candidates produced by the HATSouth survey. We show that this important step in discovering planets produces log g and T eff parameters at a precision suitable for efficient candidate vetting, as well as efficiently identifying stellar mass eclipsing binaries with radial velocity semi-amplitudes as low as 1 km s-1. The HATSouth network is operated by a collaboration consisting of Princeton University (PU), the Max Planck Institute für Astronomie (MPIA), and the Australian National University (ANU). The station at Las Campanas Observatory (LCO) of the Carnegie Institute is operated by PU in conjunction with collaborators at the Pontificia Universidad Católica de Chile (PUC), the station at the High Energy Spectroscopic Survey (HESS) site is operated in conjunction with MPIA, and the station at Siding Spring Observatory (SSO) is operated jointly with ANU.

  16. Hermitian hat wavelet design for singularity detection in the Paraguay river-level data analyses

    Science.gov (United States)

    Szu, Harold H.; Hsu, Charles C.; Sa, Leonardo D.; Li, Weigang

    1997-04-01

    The direct differentiation of a noisy signal ds/dt is known to be inaccurate. Differentiation can be improved by employing the Dirac (delta) -function introduced into a convolution product denoted by (direct product) and then integrated by parts: ds/dt equals ds/dt (direct product) (delta) equals - s (direct product) d(delta) /dt. The Schwartz Gaussian representation of the delta function is then explicitly used in the differentiation. It turns out that such a convolution approach to the first and the second derivatives produces a pair of mother wavelets the combination of which is the complex generalization of the Mexican hat called a Hermitian hat wavelet. It is shown that the Hermitian filter is a single oscillation wavelet having much lower frequency bandwidth than the Mortlet or Gabor wavelet. As a result of Nyquist theorem, a fewer number of grid points would be needed for the discrete convolution operation. Therefore, the singularity characteristic will not be overly smeared and the noise can be smoothed away. The phase plot of the Hermitian wavelet transform in terms of the time scale and frequency domains reveal a bifurcation discontinuity of a noisy cusp singularity at the precise location of the singularity as well as the scale nature of the underlying dynamics. This phase plot is defined as (theta) (t/a) equals tan-1 [(ds/dt)/(-d2s/dt2] equals tan-1 [((d(delta) (t/a)dt) (direct product) s)/((d2(delta) (t/a)/dt2) (direct product) s)] applied to a real world data of the Paraguay river levels.

  17. A redox-active tri-star molecule: merging of TTF and HAT chemistry.

    Science.gov (United States)

    Jia, Chunyang; Liu, Shi-Xia; Tanner, Christian; Leiggener, Claudia; Sanguinet, Lionel; Levillain, Eric; Leutwyler, Samuel; Hauser, Andreas; Decurtins, Silvio

    2006-05-07

    A planar pi-conjugated heteroaromatic molecule 1 has been synthesized and fully characterized; it combines two characteristics, a charge-transfer transition originating from its inherent donor-acceptor nature in its neutral state and an intervalence charge-transfer transition in its 1(2+) mixed-valence state.

  18. Structure of the Hat Creek graben region: Implications for the structure of the Hat Creek graben and transfer of right-lateral shear from the Walker Lane north of Lassen Peak, northern California, from gravity and magnetic anomalies

    Science.gov (United States)

    Langenheim, Victoria; Jachens, Robert C.; Clynne, Michael A.; Muffler, L. J. Patrick

    2016-01-01

    Interpretation of magnetic and new gravity data provides constraints on the geometry of the Hat Creek Fault, the amount of right-lateral offset in the area between Mt. Shasta and Lassen Peak, and confirmation of the influence of pre-existing structure on Quaternary faulting. Neogene volcanic rocks coincide with short-wavelength magnetic anomalies of both normal and reversed polarity, whereas a markedly smoother magnetic field occurs over the Klamath Mountains and its Paleogene cover. Although the magnetic field over the Neogene volcanic rocks is complex, the Hat Creek Fault, which is one of the most prominent normal faults in the region and forms the eastern margin of the Hat Creek Valley, is marked by the eastern edge of a north-trending magnetic and gravity high 20-30 km long. Modeling of these anomalies indicates that the fault is a steeply dipping (~75-85°) structure. The spatial relationship of the fault as modeled by the potential-field data, the youngest strand of the fault, and relocated seismicity suggests that deformation continues to step westward across the valley, consistent with a component of right-lateral slip in an extensional environment. Filtered aeromagnetic data highlight a concealed magnetic body of Mesozoic or older age north of Hat Creek Valley. The body’s northwest margin strikes northeast and is linear over a distance of ~40 km. Within the resolution of the aeromagnetic data (1-2 km), we discern no right-lateral offset of this body. Furthermore, Quaternary faults change strike or appear to end, as if to avoid this concealed magnetic body and to pass along its southeast edge, suggesting that pre-existing crustal structure influenced younger faulting, as previously proposed based on gravity data.

  19. Red Hat Linux 9.0中的磁盘配额设计与实现%The Design and Realization of the Magnetic - Dise quota of Red Hat Linux 9.0

    Institute of Scientific and Technical Information of China (English)

    李维仙; 赵京涛; 李国锋

    2005-01-01

    文章介绍了在Red Hat Linux 9.0中实施磁盘配额的必要性,实现磁盘配额的条件,以及如何实施磁盘配额和配额管理,从而能够实现对用户空间的限制,避免了硬盘空间的浪费.

  20. A model for the postcollapse equilibrium of cosmological structure truncated isothermal spheres from top-hat density perturbations

    CERN Document Server

    Shapiro, P R; Raga, A C; Shapiro, Paul R.; Iliev, Ilian; Raga, Alejandro C.

    1998-01-01

    The postcollapse structure of objects which form by gravitational condensation out of the expanding cosmological background universe is a key element in the theory of galaxy formation. Towards this end, we have reconsidered the outcome of the nonlinear growth of a uniform, spherical density perturbation in an unperturbed background universe - the cosmological ``top-hat'' problem. We adopt the usual assumption that the collapse to infinite density at a finite time predicted by the top-hat solution is interrupted by a rapid virialization caused by the growth of small-scale inhomogeneities in the initial perturbation. We replace the standard description of the postcollapse object as a uniform sphere in virial equilibrium by a more self-consistent one as a truncated, nonsingular, isothermal sphere in virial and hydrostatic equilibrium, including for the first time a proper treatment of the finite-pressure boundary condition on the sphere. The results differ significantly from both the uniform sphere and the singu...

  1. Detection of DNA Hypomethylation Mediated Floral Induction in Longan and Spinach Using the HAT-RAPD Technique

    Directory of Open Access Journals (Sweden)

    S. Anuntalabhochai

    2009-01-01

    Full Text Available In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa, petunia (Petunia hybrida, spinach (Spinacea oleracea L. and longan (Dimocarpus longan Lour., which were treated using 5-azacytidine, potassium chlorate (KClO3 and a low temperature induction. Rice and petunia were chosen to be induced because in these species it is known that 5-azacytidine is able to induce hypomethylation in their genomes leading to morphological changes, in particular floral induction, in the developing plants. Using the HAT-RAPD technique, DNA methylation was detected using the restriction enzymes HpaII and MspI in rice and petunia (as expected and in spinach, but was found to be absent in longan, which suggests that floral induction in longan is undetectable in longan.

  2. Broad-band spectrophotometry of HAT-P-32 b: search for a scattering signature in the planetary spectrum

    Science.gov (United States)

    Mallonn, M.; Bernt, I.; Herrero, E.; Hoyer, S.; Kirk, J.; Wheatley, P. J.; Seeliger, M.; Mackebrandt, F.; von Essen, C.; Strassmeier, K. G.; Granzer, T.; Künstler, A.; Dhillon, V. S.; Marsh, T. R.; Gaitan, J.

    2016-11-01

    Multicolour broad-band transit observations offer the opportunity to characterize the atmosphere of an extrasolar planet with small- to medium-sized telescopes. One of the most favourable targets is the hot Jupiter HAT-P-32 b. We combined 21 new transit observations of this planet with 36 previously published light curves for a homogeneous analysis of the broad-band transmission spectrum from the Sloan u' band to the Sloan z' band. Our results rule out cloud-free planetary atmosphere models of solar metallicity. Furthermore, a discrepancy at reddest wavelengths to previously published results makes a recent tentative detection of a scattering feature less likely. Instead, the available spectral measurements of HAT-P-32 b favour a completely flat spectrum from the near-UV to the near-IR. A plausible interpretation is a thick cloud cover at high altitudes.

  3. Broad-band spectrophotometry of HAT-P-32 b: Search for a scattering signature in the planetary spectrum

    CERN Document Server

    Mallonn, M; Herrero, E; Hoyer, S; Kirk, J; Wheatley, P J; Seeliger, M; Mackebrandt, F; von Essen, C; Strassmeier, K G; Granzer, T; Künstler, A; Dhillon, V S; Marsh, T R; Gaitan, J

    2016-01-01

    Multi-colour broad-band transit observations offer the opportunity to characterise the atmosphere of an extrasolar planet with small- to medium-sized telescopes. One of the most favourable targets is the hot Jupiter HAT-P-32 b. We combined 21 new transit observations of this planet with 36 previously published light curves for a homogeneous analysis of the broad-band transmission spectrum from the Sloan u' band to the Sloan z' band. Our results rule out cloud-free planetary atmosphere models of solar metallicity. Furthermore, a discrepancy at reddest wavelengths to previously published results makes a recent tentative detection of a scattering feature less likely. Instead, the available spectral measurements of HAT-P-32 b favour a completely flat spectrum from the near-UV to the near-IR. A plausible interpretation is a thick cloud cover at high altitudes.

  4. A Damage Tolerance Comparison of Composite Hat-Stiffened and Honeycomb Sandwich Structure for Launch Vehicle Interstage Applications

    Science.gov (United States)

    Nettles, A. T.

    2011-01-01

    In this study, a direct comparison of the compression-after-impact (CAI) strength of impact-damaged, hat-stiffened and honeycomb sandwich structure for launch vehicle use was made. The specimens used consisted of small substructure designed to carry a line load of approx..3,000 lb/in. Damage was inflicted upon the specimens via drop weight impact. Infrared thermography was used to examine the extent of planar damage in the specimens. The specimens were prepared for compression testing to obtain residual compression strength versus damage severity curves. Results show that when weight of the structure is factored in, both types of structure had about the same CAI strength for a given damage level. The main difference was that the hat-stiffened specimens exhibited a multiphase failure whereas the honeycomb sandwich structure failed catastrophically.

  5. HAT-P-2b: A Super-Massive Planet in an Eccentric Orbit Transiting a Bright Star

    CERN Document Server

    Bakos, G A; Torres, G; Fischer, D A; Latham, D W; Noyes, R W; Sasselov, D D; Mazeh, T; Shporer, A; Butler, R P; Stefanik, R P; Fernández, J M; Sozzetti, A; Pal, A; Johnson, J; Marcy, G W; Sipocz, B; Lázár, J; Papp, I; Sari, P

    2007-01-01

    We report the discovery of HAT-P-2b, a massive (Mp=8.17+/-0.72 M_Jup) planet transiting the bright (V=8.7) F8 star HD 147506, with an orbital period of 5.63 days and an eccentricity of e=0.5. From the transit light curve we determine that the radius of the planet is Rp = 1.18+/-0.16 R_Jup. HAT-P-2b has a mass about 9 times the average mass of previously-known transiting exoplanets, and a density of rho = 6.6gcm^-3, similar to that of rocky planets like the Earth. Nevertheless, its mass and radius are in accord with theories of structure of massive giant planets composed of pure H and He. The high eccentricity causes a 9-fold variation of insolation of the planet between peri- and apastron.

  6. Standard Model evaluation of $\\varepsilon_K$ using lattice QCD inputs for $\\hat{B}_K$ and $V_{cb}$

    CERN Document Server

    Bailey, Jon A; Lee, Weonjong; Park, Sungwoo

    2015-01-01

    We report the Standard Model evaluation of the indirect CP violation parameter $\\varepsilon_K$ using inputs from lattice QCD: the kaon bag parameter $\\hat{B}_K$, $\\xi_0$, $|V_{us}|$ from the $K_{\\ell 3}$ and $K_{\\mu 2}$ decays, and $|V_{cb}|$ from the axial current form factor for the exclusive decay $\\bar{B} \\to D^* \\ell \\bar{\

  7. Occurrence and HAT-RAPD analysis of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand

    OpenAIRE

    Preeyaporn Butboonchoo; Chalobol Wongsawad

    2015-01-01

    The present study determined the prevalence and distribution of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus) between November 2012 and August 2013. One hundred and twenty domestic chickens were purchased from villages in four districts of Phayao province; Mae Chai, Dok Khamtai, Chun and Chiang Kham. Morphological differences were used to identify the helminth species, and HAT-RAPD technique was used to differentiate among closely related species. The results reve...

  8. Bayesian estimation inherent in a Mexican-hat-type neural network.

    Science.gov (United States)

    Takiyama, Ken

    2016-05-01

    Brain functions, such as perception, motor control and learning, and decision making, have been explained based on a Bayesian framework, i.e., to decrease the effects of noise inherent in the human nervous system or external environment, our brain integrates sensory and a priori information in a Bayesian optimal manner. However, it remains unclear how Bayesian computations are implemented in the brain. Herein, I address this issue by analyzing a Mexican-hat-type neural network, which was used as a model of the visual cortex, motor cortex, and prefrontal cortex. I analytically demonstrate that the dynamics of an order parameter in the model corresponds exactly to a variational inference of a linear Gaussian state-space model, a Bayesian estimation, when the strength of recurrent synaptic connectivity is appropriately stronger than that of an external stimulus, a plausible condition in the brain. This exact correspondence can reveal the relationship between the parameters in the Bayesian estimation and those in the neural network, providing insight for understanding brain functions.

  9. Stellar rotation-planetary orbit period commensurability in the HAT-P-11 system

    Energy Technology Data Exchange (ETDEWEB)

    Béky, Bence; Holman, Matthew J.; Noyes, Robert W.; Kipping, David M., E-mail: bbeky@cfa.harvard.edu [Harvard-Smithsonian Center for Astrophysics, 60 Garden St., Cambridge, MA 02138 (United States)

    2014-06-10

    A number of planet host stars have been observed to rotate with a period equal to an integer multiple of the orbital period of their close planet. We expand this list by analyzing Kepler data of HAT-P-11 and finding a period ratio of 6:1. In particular, we present evidence for a long-lived spot on the stellar surface that is eclipsed by the planet in the same position four times, every sixth transit. We also identify minima in the out-of-transit light curve and confirm that their phase with respect to the stellar rotation is mostly stationary for the 48 month time frame of the observations, confirming the proposed rotation period. For comparison, we apply our methods to Kepler-17 and confirm the findings of Bonomo and Lanza that the period ratio is not exactly 8:1 in that system. Finally, we provide a hypothesis on how interactions between a star and its planet could possibly result in an observed commensurability for systems where the stellar differential rotation profile happens to include a period at some latitude that is commensurable to the planetary orbit.

  10. Intensity and Pattern of Land Surface Temperature in Hat Yai City, Thailand

    Directory of Open Access Journals (Sweden)

    Poonyanuch RUTHIRAKO

    2014-07-01

    Full Text Available Land Surface Temperature (LST is an important factor in global climate. LST is governed by surface heat fluxes, which are affected by urbanization. In order to understand urban climate, LST needs to be examined. This study aimed to investigate the intensity and pattern of LST and examine the relationships between LST and the characteristics of urban land use, indices, and population density in Hat Yai City. Landsat 5TM images were used for interpretation of land use characteristics and derivation of LST, normalized difference built-up index (NDBI and normalized vegetation index (NDVI. The characteristics of land use were classified into 4 types: commercial/high density residential, medium density residential, minimum density residential and vegetation cover/park. The average maximum and minimum LST derived from Landsat 5TM were 25.9, 33.7 and 15.8 °C, respectively. The areas with high LST were located principally in central built-up areas, slightly northwest-southeast of the study area, including the commercial center and the newly expanded residential areas. The LST pattern was well related to land use types and population density. The relationship between LST and NDVI however portrayed negative correlation, while that between LST and NDBI highlighted a positive correlation. It is concluded that NDVI and NDBI can be used to evaluate the risk of Urban Heat Island (UHI and may help city managers better prepare for possible impacts of climate change.

  11. Stellar rotation--planetary orbit period commensurability in the HAT-P-11 system

    CERN Document Server

    Béky, Bence; Kipping, David M; Noyes, Robert W

    2014-01-01

    A number of planet-host stars have been observed to rotate with a period equal to an integer multiple of the orbital period of their close planet. We expand this list by analyzing Kepler data of HAT-P-11 and finding a period ratio of 6:1. In particular, we present evidence for a long-lived spot on the stellar surface that is eclipsed by the planet in the same position four times, every sixth transit. We also identify minima in the out-of-transit lightcurve and confirm that their phase with respect to the stellar rotation is mostly stationary for the 48-month timeframe of the observations, confirming the proposed rotation period. For comparison, we apply our methods to Kepler-17 and confirm the findings of Bonomo & Lanza (2012) that the period ratio is not exactly 8:1 in that system. Finally, we provide a hypothesis on how interactions between a star and its planet could possibly result in an observed commensurability for systems where the stellar differential rotation profile happens to include a period a...

  12. HAT-P-7: A Retrograde or Polar Orbit, and a Second Planet

    CERN Document Server

    Winn, Joshua N; Albrecht, Simon; Howard, Andrew W; Marcy, Geoffrey W; Crossfield, Ian J; Holman, Matthew J

    2009-01-01

    We show that the exoplanet HAT-P-7b has an extremely tilted orbit, with a true angle of at least 86 degrees with respect to its parent star's equatorial plane, and a strong possibility of retrograde motion. We also report evidence for a second planet in a more distant orbit. The evidence for the unparalleled orbit and the additional planet is based on precise observations of the star's apparent radial velocity. The anomalous radial velocity due to rotation (the Rossiter-McLaughlin effect) was found to be a blueshift during the first half of the transit and a redshift during the second half, an inversion of the usual effect, implying that the angle between the sky-projected orbital and stellar angular momentum vectors is 182.5 +/- 9.4 deg. The second planet is implicated by excess radial-velocity variation of the host star over 2 yr. Possibly, the second planet tilted the orbit of the inner planet through a close encounter or the Kozai effect.

  13. Improvement and evaluation of polymer-matrix composite panels with hat stiffeners

    Directory of Open Access Journals (Sweden)

    Li S. J.

    2016-01-01

    Full Text Available Hat-stiffened composite panels fabricated by co-curing technologies are widely used in the fuselage panel due to the good structural stability and high efficiency of axial load transferring. The bonding capability between the stiffener and skin is a primary criterion to assess the co-curing quality. In this paper, two reinforcement technologies of filling filler in the triangle region and adding split-stopping tape between the stiffener and skin were employed to improve the bonding capability. Effect of filler and split-stopping tape on the interface strength was analyzed, and the optimal size range of the filler and split-stopping tape were obtained. To improve the universality of application for the two reinforcement techniques, the filling coefficient of 0.62~0.77 and the split-stopping tape width coefficient of 0.56~0.67 were obtained by calculation. Results of the study can be used to develop other kinds of stiffened panels and will ultimately lead to optimized skin/stiffener designs.

  14. Hospital Acquired Thrombosis (HAT) Prevention in an Acute Hospital; A Single Centre Cross-Sectional Study

    LENUS (Irish Health Repository)

    Khan, MI

    2017-04-01

    Evidence based guidelines are effective in reducing incidence of venous thromboembolism (VTE) which is associated with morbidly, mortality and economic burden. This study aimed to identify the proportion of inpatients who had a VTE risk assessment (RA) performed and who received thromboprophylaxis (TP), in Cork University Hospital. There was no structured RA tool at the time; information was obtained from medical and drug charts to ascertain if a RA was performed. Patients were then RA by researchers and stratified as per NICE guidelines and the proportion who received TP was calculated. One thousand and nineteen inpatients were screened. Risk was documented in 24% of cases. TP was prescribed in 43.2% of inpatients. Following application of a RA tool >80% were at high risk of VTE with low risk of bleeding with TP prescription in 46.3% of inpatients. A national collaborative effort should be encouraged to develop a standardized approach for safe RA of inpatients and prescription of TP for prevention of HAT

  15. The retrograde orbit of the HAT-P-6b exoplanet

    Science.gov (United States)

    Hébrard, G.; Ehrenreich, D.; Bouchy, F.; Delfosse, X.; Moutou, C.; Arnold, L.; Boisse, I.; Bonfils, X.; Díaz, R. F.; Eggenberger, A.; Forveille, T.; Lagrange, A.-M.; Lovis, C.; Pepe, F.; Perrier, C.; Queloz, D.; Santerne, A.; Santos, N. C.; Ségransan, D.; Udry, S.; Vidal-Madjar, A.

    2011-03-01

    We observed the transit of the HAT-P-6b exoplanet across its host star with the SOPHIE spectrograph (OHP, France). The resulting stellar radial velocities display the Rossiter-McLaughlin anomaly and reveal a retrograde orbit: the planetary orbital spin and the stellar rotational spin point in approximately opposite directions. A fit to the anomaly measures a sky-projected angle λ = 166° ± 10° between these two spin axes. All seven known retrograde planets are hot Jupiters with masses Mp 4 MJup) are prograde but misaligned. Different mechanisms may therefore be responsible for planetary obliquities above and below ~3.5 MJup. Based on observations collected with the SOPHIE spectrograph on the 1.93-m telescope at Observatoire de Haute-Provence (CNRS), France, by the SOPHIE Consortium (program 10A.PNP.CONS).SOPHIE radial velocities are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/527/L11

  16. Further constraints on the optical transmission spectrum of HAT-P-1b

    CERN Document Server

    Montalto, M; Santos, N C; Desidera, S; Martins, J H C; Figueira, P; Alonso, R; .,

    2015-01-01

    We report on novel observations of HAT-P-1 aimed at constraining the optical transmission spectrum of the atmosphere of its transiting Hot-Jupiter exoplanet. Ground-based differential spectrophotometry was performed over two transit windows using the DOLORES spectrograph at the Telescopio Nazionale Galileo (TNG). Our measurements imply an average planet to star radius ratio equal to $\\rm R_p/R_{\\star}$=(0.1159$\\pm$0.0005). This result is consistent with the value obtained from recent near infrared measurements of this object but differs from previously reported optical measurements being lower by around 4.4 exoplanet scale heights. Analyzing the data over 5 different spectral bins 600\\AA$\\,$ wide we observed a single peaked spectrum (3.7 $\\rm\\sigma$ level) with a blue cut-off corresponding to the blue edge of the broad absorption wing of sodium and an increased absorption in the region in between 6180-7400\\AA. We also infer that the width of the broad absorption wings due to alkali metals is likely narrower t...

  17. Bayesian estimation inherent in a Mexican-hat-type neural network

    Science.gov (United States)

    Takiyama, Ken

    2016-05-01

    Brain functions, such as perception, motor control and learning, and decision making, have been explained based on a Bayesian framework, i.e., to decrease the effects of noise inherent in the human nervous system or external environment, our brain integrates sensory and a priori information in a Bayesian optimal manner. However, it remains unclear how Bayesian computations are implemented in the brain. Herein, I address this issue by analyzing a Mexican-hat-type neural network, which was used as a model of the visual cortex, motor cortex, and prefrontal cortex. I analytically demonstrate that the dynamics of an order parameter in the model corresponds exactly to a variational inference of a linear Gaussian state-space model, a Bayesian estimation, when the strength of recurrent synaptic connectivity is appropriately stronger than that of an external stimulus, a plausible condition in the brain. This exact correspondence can reveal the relationship between the parameters in the Bayesian estimation and those in the neural network, providing insight for understanding brain functions.

  18. Point Source Detection using the Spherical Mexican Hat Wavelet on simulated all-sky Planck maps

    CERN Document Server

    Vielva, P; Gallegos, J E; Toffolatti, L; Sanz, J L

    2003-01-01

    We present an estimation of the point source (PS) catalogue that could be extracted from the forthcoming ESA Planck mission data. We have applied the Spherical Mexican Hat Wavelet in simulated all-sky maps that include CMB, Galactic emission, thermal Sunyaev-Zel'dovich effect and PS emission, as well as instrumental white noise. We have developed an algorithm focused on a fast optimal scale determination, that is crucial to achieve a PS catologue with a large number of detections and a low flux limit. An important effort has been also done to reduce the CPU time processor for spherical harmonic trans formation, in order to perform the PS detection in a reasonable time. The presented algorithm is able to provide a PS catalogue above fluxes: 1.39 Jy (857 GHz), 0.84 Jy (545 GHz), 0.30 Jy (353 GHz), 0.16 Jy (217 GHz), 0.17 Jy (143 GHz), 0.19 Jy (100 GHz HFI), 0.22 Jy (100 GHz LFI), 0.28 Jy (70 GHz), 0.33 Jy (44 GHz) and 0.37 Jy (30 GHz). We detect around 36700 PS at the highest frequency Planck channel and 2200 a...

  19. Anion-π recognition between [M(CN)6](3-) complexes and HAT(CN)6: structural matching and electronic charge density modification.

    Science.gov (United States)

    Kobylarczyk, Jedrzej; Pinkowicz, Dawid; Srebro-Hooper, Monika; Hooper, James; Podgajny, Robert

    2017-03-14

    Hexacyanidometalates (M = Fe(III), Co(III)) and multisite anion receptor HAT(CN)6 (1,4,5,8,9,11-hexaazatriphenylenehexacarbonitrile) recognize each other in acetonitrile solution and self-assemble into the novel molecular networks (PPh4)3[M(CN)6][HAT(CN)6] (M = Fe, 1; Co, 2) and (AsPh4)3[M(CN)6][HAT(CN)6]·2MeCN·H2O (M = Fe, 3; Co, 4). 1-4 contain the stacked columns {[M(CN)6](3-);[HAT(CN)6]}∞ separated by the organic cations. All of the M-C[triple bond, length as m-dash]N vectors point collectively towards the centroids of pyrazine rings on neighboring HAT(CN)6 molecules, with Ncyanidecentroidpyrazine distances that are under 3 Å. The directional character and structural parameters of the new supramolecular synthons correspond to collective triple anion-π interactions between the CN(-) ligands of the metal complexes and the π-deficient areas of HAT(CN)6. Physicochemical characterisation (IR spectroscopy, UV-Vis spectroscopy, cyclic voltammetry) and dispersion-corrected DFT studies reveal the dominating charge-transfer (CT) and polarisation characters of the interactions. The electronic density flow occurs from the CN(-) ligands of [M(CN)6](3-) to the HAT(CN)6 orbital systems and further, toward the peripheral -CN groups of HAT(CN)6. Solid-state DFT calculations determined the total interaction energy of HAT(CN)6 to be ca. -125 kcal mol(-1), which gives ca. -15 kcal mol(-1) per one CN(-)HAT(CN)6 contact after subtraction of the interaction with organic cations. The UV-Vis electronic absorption measurements prove that the intermolecular interactions persist in solution and suggest a 1 : 1 composition of the anion-π {[M(CN)6](3-);[HAT(CN)6]} chromophore, with the formation constant Kadd = (5.8 ± 6) × 10(2) dm(3) mol(-1) and the molar absorption coefficient εadd = 180 ± 9 cm(-1) dm(3) mol(-1) at 600 nm, as estimated from concentration-dependent studies.

  20. HAT-P-11b: A Super-Neptune Planet Transiting a Bright K Star in the Kepler Field

    CERN Document Server

    Bakos, G Á; Pál, A; Hartman, J; Kovács, Géza; Noyes, R W; Latham, D W; Sasselov, D D; Sipőcz, B; Esquerdo, G A; Fischer, D A; Johnson, J A; Marcy, G W; Butler, R P; Isaacson, H; Howard, A; Vogt, S; Kovács, Gábor; Fernández, J; Moór, A; Stefanik, R P; Lázár, J; Papp, I; Sári, P

    2009-01-01

    We report on the discovery of HAT-P-11b, the smallest radius transiting extrasolar planet (TEP), and the first hot Neptune discovered to date by transit searches. HAT-P-11b orbits the bright (V=9.59) and metal rich ([Fe=H] = +0.31 +/- 0.05) K4 dwarf star GSC 03561-02092 with P = 4.8878162 +/- 0.0000071 days and produces a transit signal with depth of 4.2 mmag; the shallowest found by transit searches that is due to a confirmed planet. We present a global analysis of the available photometric and radial-velocity data that result in stellar and planetary parameters, with simultaneous treatment of systematic variations. The planet, like its near-twin GJ 436b, is somewhat larger than Neptune (17 Mearth, 3.8 Rearth) both in mass Mp = 0.081 +/- 0.009 MJup (25.8 +/- 2.9 Mearth) and radius Rp = 0.422 +/- 0.014 RJup (4.73 +/- 0.16 Rearth). HAT-P-11b orbits in an eccentric orbit with e = 0.198 +/- 0.046 and omega = 355.2 +/- 17.3 deg, causing a reflex motion of its parent star with amplitude 11.6 +/- 1.2 m/s, a challen...

  1. Fluctuations of hi-hat timing and dynamics in a virtuoso drum track of a popular music recording.

    Directory of Open Access Journals (Sweden)

    Esa Räsänen

    Full Text Available Long-range correlated temporal fluctuations in the beats of musical rhythms are an inevitable consequence of human action. According to recent studies, such fluctuations also lead to a favored listening experience. The scaling laws of amplitude variations in rhythms, however, are widely unknown. Here we use highly sensitive onset detection and time series analysis to study the amplitude and temporal fluctuations of Jeff Porcaro's one-handed hi-hat pattern in "I Keep Forgettin'"-one of the most renowned 16th note patterns in modern drumming. We show that fluctuations of hi-hat amplitudes and interbeat intervals (times between hits have clear long-range correlations and short-range anticorrelations separated by a characteristic time scale. In addition, we detect subtle features in Porcaro's drumming such as small drifts in the 16th note pulse and non-trivial periodic two-bar patterns in both hi-hat amplitudes and intervals. Through this investigation we introduce a step towards statistical studies of the 20th and 21st century music recordings in the framework of complex systems. Our analysis has direct applications to the development of drum machines and to drumming pedagogy.

  2. Self adaptive multi-scale morphology AVG-Hat filter and its application to fault feature extraction for wheel bearing

    Science.gov (United States)

    Deng, Feiyue; Yang, Shaopu; Tang, Guiji; Hao, Rujiang; Zhang, Mingliang

    2017-04-01

    Wheel bearings are essential mechanical components of trains, and fault detection of the wheel bearing is of great significant to avoid economic loss and casualty effectively. However, considering the operating conditions, detection and extraction of the fault features hidden in the heavy noise of the vibration signal have become a challenging task. Therefore, a novel method called adaptive multi-scale AVG-Hat morphology filter (MF) is proposed to solve it. The morphology AVG-Hat operator not only can suppress the interference of the strong background noise greatly, but also enhance the ability of extracting fault features. The improved envelope spectrum sparsity (IESS), as a new evaluation index, is proposed to select the optimal filtering signal processed by the multi-scale AVG-Hat MF. It can present a comprehensive evaluation about the intensity of fault impulse to the background noise. The weighted coefficients of the different scale structural elements (SEs) in the multi-scale MF are adaptively determined by the particle swarm optimization (PSO) algorithm. The effectiveness of the method is validated by analyzing the real wheel bearing fault vibration signal (e.g. outer race fault, inner race fault and rolling element fault). The results show that the proposed method could improve the performance in the extraction of fault features effectively compared with the multi-scale combined morphological filter (CMF) and multi-scale morphology gradient filter (MGF) methods.

  3. Occurrence and HAT-RAPD analysis of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand.

    Science.gov (United States)

    Butboonchoo, Preeyaporn; Wongsawad, Chalobol

    2017-01-01

    The present study determined the prevalence and distribution of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus) between November 2012 and August 2013. One hundred and twenty domestic chickens were purchased from villages in four districts of Phayao province; Mae Chai, Dok Khamtai, Chun and Chiang Kham. Morphological differences were used to identify the helminth species, and HAT-RAPD technique was used to differentiate among closely related species. The results revealed that the total prevalence of infection was 99.2%. Cestode and nematode infections showed the highest prevalence in rainy season, while trematode infections were low and only found in hot season. The species and their prevalence were: Ascaridia galli (50.8%), Heterakis gallinarum (86.7%), Prosthogonimus macrorchis (1.7%), Echinostoma revolutum (0.8%), Raillietina echinobothrida (48.3%), Raillietina tetragona (57.5%), Raillietina cesticillus (12.5%), Raillietina sp. (35.8%), Cotugnia chiangmaii (14.2%) and Cotugnia sp. (32.5%). The prevalence of helminth infections did not differ significantly between male and female chickens. HAT-RAPD analysis, the specific fragment of 400 and 250 bp indicated that Raillietina sp. and Cotugnia sp. found, respectively, differ from other closely related species. This study has confirmed that HAT-RAPD technique can be used to differentiate among related species combined with morphological observations.

  4. Occurrence and HAT-RAPD analysis of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus in Phayao province, northern Thailand

    Directory of Open Access Journals (Sweden)

    Preeyaporn Butboonchoo

    2017-01-01

    Full Text Available The present study determined the prevalence and distribution of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus between November 2012 and August 2013. One hundred and twenty domestic chickens were purchased from villages in four districts of Phayao province; Mae Chai, Dok Khamtai, Chun and Chiang Kham. Morphological differences were used to identify the helminth species, and HAT-RAPD technique was used to differentiate among closely related species. The results revealed that the total prevalence of infection was 99.2%. Cestode and nematode infections showed the highest prevalence in rainy season, while trematode infections were low and only found in hot season. The species and their prevalence were: Ascaridia galli (50.8%, Heterakis gallinarum (86.7%, Prosthogonimus macrorchis (1.7%, Echinostoma revolutum (0.8%, Raillietina echinobothrida (48.3%, Raillietina tetragona (57.5%, Raillietina cesticillus (12.5%, Raillietina sp. (35.8%, Cotugnia chiangmaii (14.2% and Cotugnia sp. (32.5%. The prevalence of helminth infections did not differ significantly between male and female chickens. HAT-RAPD analysis, the specific fragment of 400 and 250 bp indicated that Raillietina sp. and Cotugnia sp. found, respectively, differ from other closely related species. This study has confirmed that HAT-RAPD technique can be used to differentiate among related species combined with morphological observations.

  5. Widefield imaging of upconverting nanoparticles on epifluorescence microscopes adapted for laser illumination with top-hat profile

    Science.gov (United States)

    Mrazek, Jiri; Pospisilova, Martina; Svozil, Vit; Cadek, Ondrej; Nesporova, Kristina; Sulakova, Romana; Brandejsova, Martina; Vranova, Jana; Velebny, Vladimir

    2016-05-01

    We describe a modification of epifluorescence microscopes that allows quantitative widefield imaging of samples labeled by upconverting nanoparticles (UCNP). A top-hat illumination profile on the sample was achieved with a 980-nm laser diode by using tandem microlens arrays, a moving diffuser and a telescope, which adjusts the top-hat area to the field of view. Illumination homogeneity is a critical factor for imaging of UCNP since the intensity of their luminescence typically scales with the second power of the excitation intensity. Our illuminator is combined with the epifluorescence attachment of the microscope, allowing easy switching between observation of UCNP and traditional fluorescent dyes. Illumination profile homogeneity of about 98% was measured for objectives with magnification from 4× to 100×, and the top-hat profile was also obtained with phase contrast objectives. We demonstrate capability of the illuminator by evaluating in vitro uptake of UCNP encapsulated in oleyl-hyaluronan micelles into breast cancer cells. Micelles bearing the targeting peptide were about an order of magnitude more efficient than nontargeted micelles.

  6. Ideologiekritik des E-Learnings. Welchen Nutzen hat die Einführung von E-Learning?

    Directory of Open Access Journals (Sweden)

    Katharina Kaiser-Müller

    2015-03-01

    Full Text Available Vor etwa sieben Jahren hat das damalige Bundesministerium für Unterricht, Kunst und Kultur (bm:ukk, das seit 2014 Bundesministerium für Bildung und Frauen (BMBF genannt wird, verschiedene Maßnahmen im Blick auf neuen Medien – insbesondere im Blick auf das E-Learning – gesetzt, um einen Beitrag zur Vorbereitung der Lernenden auf eine Informations- und Wissensgesellschaft zu leisten. E-Learning wurde als Erfolgsfaktor für die Zukunft gesehen und mit der Begründung, es fördere das individuelle und selbst gesteuerte Lernen, gefördert. Die zentrale Frage ist dabei: Welchen Nutzen hat die Einführung von E-Learning? In diesem Artikel wird dahingehend aufgezeigt, dass E-Learning nicht wegen pädagogischem Bedarf, sondern durch ministeriell gesetzte Maßnahmen eingeführt wurde und dadurch – wenn auch nicht bewusst und schon gar nicht als solches kommuniziert – neoliberale Strukturen durchgesetzt wurden. Die Einführung von E-Learning hat somit für deren Akzeptanz gesorgt.

  7. Energy versus centrality dependence of the jet quenching parameter hat{q} at RHIC and LHC: a new puzzle?

    Science.gov (United States)

    Andrés, Carlota; Armesto, Néstor; Luzum, Matthew; Salgado, Carlos A.; Zurita, Pía

    2016-09-01

    The central goal of jet quenching studies in high-energy nuclear collisions is the characterization of those QCD medium properties that are accessible by these probes. Most of the discussion in the last years has been focused on the determination of the jet quenching parameter, hat{q}. We present here an extraction of this parameter using data of inclusive particle suppression at RHIC and LHC energies for different centralities. Our approach consists in fitting a K factor that quantifies the departure of this parameter from an ideal estimate, K≡ hat{q}/(2ɛ ^{3/4}), where hat{q} is determined by the local medium quantities as provided by hydrodynamical calculations. We find that this K factor is larger at RHIC than at the LHC, as obtained already in previous analyses, but, surprisingly, it is almost independent of the centrality of the collision. Taken at face value, the K factor would not depend on the local properties of the medium as energy density or temperature, but on global collision quantities such as the center of mass energy. This is a very intriguing, unexpected possibility for which we cannot yet provide a clear interpretation. We also comment on the limitations of the formalism that may affect this conclusion.

  8. Energy versus centrality dependence of the jet quenching parameter $\\hat q$ at RHIC and LHC: a new puzzle?

    CERN Document Server

    Andrés, Carlota; Luzum, Matthew; Salgado, Carlos A; Zurita, Pía

    2016-01-01

    The central goal of jet quenching studies in high-energy nuclear collisions is the characterization of those QCD medium properties that are accessible by these probes. Most of the discussion in the last years has been focused on the determination of the jet quenching parameter, $\\hat q$. We present here an extraction of this parameter using data of inclusive particle suppression at RHIC and LHC energies for different centralities. Our approach consists of fitting a $K$ factor that quantifies the departure of this parameter from an ideal estimate, $K\\equiv \\hat q/(2\\epsilon^{3/4})$, where $\\hat q$ is determined by the local medium quantities as provided by hydrodynamical calculations. We find that this $K$ factor is larger at RHIC than at the LHC, as obtained already in previous analyses, but, surprisingly, it is almost independent of the centrality of the collision. Taken at face value, the $K$ factor would not depend on the local properties of the medium as energy density or temperature, but on global collis...

  9. An Improved Top-Hat Filter with Sloped Brim for Extracting Ground Points from Airborne Lidar Point Clouds

    Directory of Open Access Journals (Sweden)

    Yong Li

    2014-12-01

    Full Text Available Airborne light detection and ranging (lidar has become a powerful support for acquiring geospatial data in numerous geospatial applications and analyses. However, the process of extracting ground points accurately and effectively from raw point clouds remains a big challenge. This study presents an improved top-hat filter with a sloped brim to enhance the robustness of ground point extraction for complex objects and terrains. The top-hat transformation is executed and the elevation change intensity of the transitions between the obtained top-hats and outer brims is inspected to suppress the omission error caused by protruding terrain features. Finally, the nonground objects of complex structures, such as multilayer buildings, are identified by the brim filter that is extended outward. The performance of the proposed filter in various environments is evaluated using diverse datasets with difficult cases. The comparison of the proposed filter with the commercial software Terrasolid TerraScan and other popular filtering algorithms demonstrates the applicability and effectiveness of this filter. Experimental results show that the proposed filter has great promise in terms of its application in various types of landscapes. Abrupt terrain features with dramatic elevation changes are well preserved, and diverse objects with complicated shapes are effectively removed. This filter has minimal omission and commission error oscillation for different test areas and thus demonstrates a stable and reliable performance in diverse landscapes. In addition, the proposed algorithm has high computational efficiency because of its simple and efficient data structure and implementation.

  10. The GTC exoplanet transit spectroscopy survey. IV. Confirmation of the flat transmission spectrum of HAT-P-32b

    CERN Document Server

    Nortmann, L; Murgas, F; Dreizler, S; Iro, N; Cabrera-Lavers, A

    2016-01-01

    We observed the hot Jupiter HAT-P-32b (also known as HAT-P-32Ab) to determine its optical transmission spectrum by measuring the wavelength-dependent planet-to-star radius ratios in the region between 518 - 918 nm. We used the OSIRIS instrument at the GTC in long slit spectroscopy mode, placing HAT-P-32 and a reference star in the same slit and obtaining a time series of spectra covering two transit events. Using the best quality data set, we were able to yield 20 narrow-band transit light curves, with each passband spanning a 20 nm wide interval. After removal of all systematic noise signals and light curve modeling the uncertainties for the resulting radius ratios lie between 337 and 972 ppm. The radius ratios show little variation with wavelength suggesting a high altitude cloud layer masking any atmospheric features. Alternatively, a strong depletion in alkali metals or a much smaller than expected planetary atmospheric scale height could be responsible for the lack of atmospheric features. Our result of ...

  11. A model for the post-collapse equilibrium of cosmological structure: truncated isothermal spheres from top-hat density perturbations

    Science.gov (United States)

    Shapiro, Paul R.; Iliev, Ilian T.; Raga, Alejandro C.

    1999-07-01

    The post-collapse structure of objects that form by gravitational condensation out of the expanding cosmological background universe is a key element in the theory of galaxy formation. Towards this end, we have reconsidered the outcome of the non-linear growth of a uniform, spherical density perturbation in an unperturbed background universe - the cosmological `top-hat' problem. We adopt the usual assumption that the collapse to infinite density at a finite time predicted by the top-hat solution is interrupted by a rapid virialization caused by the growth of small-scale inhomogeneities in the initial perturbation. We replace the standard description of the post-collapse object as a uniform sphere in virial equilibrium by a more self-consistent one as a truncated, non-singular, isothermal sphere in virial and hydrostatic equilibrium, including for the first time a proper treatment of the finite-pressure boundary condition on the sphere. The results differ significantly from both the uniform sphere and the singular isothermal sphere approximations for the post-collapse objects. The virial temperature that results is more than twice the previously used `standard value' of the post-collapse uniform sphere approximation, but 1.4 times smaller than that of the singular, truncated isothermal sphere approximation. The truncation radius is 0.554 times the radius of the top-hat at maximum expansion, and the ratio of the truncation radius to the core radius is 29.4, yielding a central density that is 514 times greater than at the surface and 1.8x10^4 times greater than that of the unperturbed background density at the epoch of infinite collapse predicted by the top-hat solution. For the top-hat fractional overdensity delta_L predicted by extrapolating the linear solution into the non-linear regime, the standard top-hat model assumes that virialization is instantaneous at delta_Ldelta_c=1.686 i.e. the epoch at which the non-linear top-hat reaches infinite density. The surface

  12. Identification of a high frequency transposon induced by tissue culture, nDaiZ, a member of the hAT family in rice.

    Science.gov (United States)

    Huang, Jian; Zhang, Kewei; Shen, Yi; Huang, Zejun; Li, Ming; Tang, Ding; Gu, Minghong; Cheng, Zhukuan

    2009-03-01

    Recent completion of rice genome sequencing has revealed that more than 40% of its genome consists of repetitive sequences, and most of them are related to inactive transposable elements. In the present study, a transposable element, nDaiZ0, which is induced by tissue culture with high frequency, was identified by sequence analysis of an allelic line of the golden hull and internode 2 (gh2) mutant, which was integrated into the forth exon of GH2. The 528-bp nDaiZ0 has 14-bp terminal inverted repeats (TIRs), and generates an 8-bp duplication of its target sites (TSD) during its mobilization. nDaiZs are non-autonomous transposons and have no coding capacity. Bioinformatics analysis and southern blot hybridization showed that at least 16 copies of nDaiZ elements exist in the japonica cultivar Nipponbare genome and 11 copies in the indica cultivar 93-11 genome. During tissue culture, only one copy, nDaiZ9, located on chromosome 5 in the genome of Nipponbare can be activated with its transposable frequency reaching 30%. However, nDaiZ9 was not present in the 93-11 genome. The larger elements, DaiZs, were further identified by database searching using nDaiZ0 as a query because they share similar TIRs and subterminal sequences. DaiZ can also generate an 8-bp TSD. DaiZ elements contain a conserved region with a high similarity to the hAT dimerization motif, suggesting that the nDaiZ-DaiZ transposon system probably belongs to the hAT superfamily of class II transposons. Phylogenetic analysis indicated that it is a new type of plant hAT-like transposon. Although nDaiZ is activated by tissue culture, the high transposable frequency indicates that it could become a useful gene tagging system for rice functional genomic studies. In addition, the mechanism of the high transposable ability of nDaiZ9 is discussed.

  13. Transmission spectroscopy of the inflated exo-Saturn HAT-P-19b

    Science.gov (United States)

    Mallonn, M.; von Essen, C.; Weingrill, J.; Strassmeier, K. G.; Ribas, I.; Carroll, T. A.; Herrero, E.; Granzer, T.; Claret, A.; Schwope, A.

    2015-08-01

    Context. Transiting highly inflated giant planets offer the possibility of characterizing their atmospheres. A fraction of the starlight passes through the high-altitude layers of the planetary atmosphere during transit. The resulting absorption is expected to be wavelength dependent for cloud-free atmospheres with an amplitude of up to 10-3 of the stellar flux, while a high-altitude cloud deck would cause a gray opacity. Aims: We observed the Saturn-mass and Jupiter-sized exoplanet HAT-P-19b to refine its transit parameters and ephemeris as well as to shed first light on its transmission spectrum. We monitored the host star over one year to quantify its flux variability and to correct the transmission spectrum for a slope caused by starspots. Methods: A transit of HAT-P-19b was observed spectroscopically with OSIRIS at the Gran Telescopio Canarias in January 2012. The spectra of the target and the comparison star covered the wavelength range from 5600 to 7600 Å. One high-precision differential light curve was created by integrating the entire spectral flux. This white-light curve was used to derive absolute transit parameters. Furthermore, a set of light curves over wavelength was formed by a flux integration in 41 wavelength channels of 50 Å width. We analyzed these spectral light curves for chromatic variations of transit depth. Results: The transit fit of the combined white-light curve yields a refined value of the planet-to-star radius ratio of 0.1390 ± 0.0012 and an inclination of 88.89 ± 0.32 deg. After a re-analysis of published data, we refine the orbital period to 4.0087844 ± 0.0000015 days. We obtain a flat transmission spectrum without significant additional absorption at any wavelength or any slope. However, our accuracy is not sufficient to significantly rule out the presence of a pressure-broadened sodium feature. Our photometric monitoring campaign allowed for an estimate of the stellar rotation period of 35.5 ± 2.5 days and an improved age

  14. Inactivation of Hepatitis B Virus Replication in Cultured Cells and In Vivo with Engineered Transcription Activator-Like Effector Nucleases

    OpenAIRE

    Bloom, Kristie; Ely, Abdullah; Mussolino, Claudio; Cathomen, Toni; Arbuthnot, Patrick

    2013-01-01

    Chronic hepatitis B virus (HBV) infection remains an important global health problem. Stability of the episomal covalently closed circular HBV DNA (cccDNA) is largely responsible for the modest curative efficacy of available therapy. Since licensed anti-HBV drugs have a post-transcriptional mechanism of action, disabling cccDNA is potentially of therapeutic benefit. To develop this approach, we engineered mutagenic transcription activator-like effector nucleases (TALENs) that target four HBV-...

  15. Sanitation conditions of clean food good taste restaurants in Hat Yai City Municipality

    Directory of Open Access Journals (Sweden)

    Sopon, A.

    2004-01-01

    Full Text Available Sanitation conditions and microbiological quality of 52 “Clean Food Good Taste” restaurants in Hat Yai city municipality were examined using a standard food sanitation survey checklist based on the Department of Health and Department of Medical Science, Ministry of Public Health. Coliform bacteria and Escherichia coli (E. coli were investigated in samples of foods and drinking water, whereas total bacterial count (TBC was carried out in samples of foods, plates, spoons, glasses and food handlers. The methods of investigation were the Most Probable Number (MPN method for coliform bacteria, E. coli and the standard plate count method for TBC. The SI-2 field test kit was used to indicate microbiological contamination, particularly coliform bacteria. It was found that 38/52 (73.1% restaurants passed all items of food sanitation standard. The food sanitation condition with the lowest number passing was the dressing of food handlers (45/52, 86.5% followed by the area for eating, preparing and cooking (47/52, 90.4%. Microbiological quality of food samples based on both MPN of coliform bacteria and E. coli was at an acceptable level in 190/202 samples (94.1%. However, in samples of drinking water only 19/52 (36.5% passed the MPN standard for coliform bacteria and 45/52 (86.5% that for E. coli. Moreover, among the 52 restaurants, the numbers (percentages passing the standard TBC in samples of plate, spoon, glass, cooker handlers and server handlers were 32 (61.5%, 27(51.9%, 20 (38.5%, 2 (3.9% and 1 (1.9%, respectively. Comparison of microbiological quality between the SI-2 test kit and MPN coliform/TBC showed no significant differences for samples of foods, but significant differences for the rest of the samples (p<0.05, t-test.

  16. Shaping the PSF to nearly top-hat profile: CHEOPS laboratory results

    Science.gov (United States)

    Magrin, Demetrio; Farinato, Jacopo; Umbriaco, Gabriele; Kumar Radhakrishnan Santhakumari, Kalyan; Bergomi, Maria; Dima, Marco; Greggio, Davide; Marafatto, Luca; Ragazzoni, Roberto; Viotto, Valentina; Munari, Matteo; Pagano, Isabella; Scandariato, Gaetano; Scuderi, Salvatore; Piotto, Giampaolo; Beck, Thomas; Benz, Willy; Broeg, Christopher; Cessa, Virginie; Fortier, Andrea; Piazza, Daniele

    2014-08-01

    Spreading the PSF over a quite large amount of pixels is an increasingly used observing technique in order to reach extremely precise photometry, such as in the case of exoplanets searching and characterization via transits observations. A PSF top-hat profile helps to minimize the errors contribution due to the uncertainty on the knowledge of the detector flat field. This work has been carried out during the recent design study in the framework of the ESA small mission CHEOPS. Because of lack of perfect flat-fielding information, in the CHEOPS optics it is required to spread the light of a source into a well defined angular area, in a manner as uniform as possible. Furthermore this should be accomplished still retaining the features of a true focal plane onto the detector. In this way, for instance, the angular displacement on the focal plane is fully retained and in case of several stars in a field these look as separated as their distance is larger than the spreading size. An obvious way is to apply a defocus, while the presence of an intermediate pupil plane in the Back End Optics makes attractive to introduce here an optical device that is able to spread the light in a well defined manner, still retaining the direction of the chief ray hitting it. This can be accomplished through an holographic diffuser or through a lenslet array. Both techniques implement the concept of segmenting the pupil into several sub-zones where light is spread to a well defined angle. We present experimental results on how to deliver such PSF profile by mean of holographic diffuser and lenslet array. Both the devices are located in an intermediate pupil plane of a properly scaled laboratory setup mimicking the CHEOPS optical design configuration.

  17. VizieR Online Data Catalog: Spectroscopy and photometry of HATS-17 (Brahm+, 2016)

    Science.gov (United States)

    Brahm, R.; Jordan, A.; Bakos, G. A.; Penev, K.; Espinoza, N.; Rabus, M.; Hartman, J. D.; Bayliss, D.; Ciceri, S.; Zhou, G.; Mancini, L.; Tan, T. G.; de Val-Borro, M.; Bhatti, W.; Csubry, Z.; Bento, J.; Henning, T.; Schmidt, B.; Rojas, F.; Suc, V.; Lazar, J.; Papp, I.; Sari, P.

    2016-07-01

    The star HATS-17 was observed by HATSouth instruments between UT 2011 April 26 and UT 2012 July 31 using the HS-2 (on 2011 Apr-2012 Jul with r filter), HS-4 (on 2011 Jul-2012 Jul with r filter), and HS-6 (on 2011 May-2012 Jul with r filter) units at Las Campanas Observatory (LCO) in Chile, the High Energy Spectroscopic Survey (H.E.S.S.) site in Namibia, and Siding Spring Observatory (SSO) in Australia, respectively. The first photometric follow-up light curve of this system was obtained with the 0.3m Perth Exoplanet Survey Telescope (PEST) located near Perth on 2015 Apr 26 with a RC filter. Another two partial transits were then acquired with the Las Cumbres Observatory Global Telescope (LCOGT) 1m telescope network, specifically with the telescope at Cerro Tololo Inter-American Observatory (CTIO) on 2015 May 13 with i filter, and with the Swope 1m coupled with the e2v camera at Las Campanas Observatory (LCO) on 2015 May 29 with i filter. Finally, two partial transits of the same event were measured with high photometric precision (~1mmag). The observations were performed with the same two telescopes that registered the previous partial transits (Swope 1m/e2v (LCO) on 2015 Jul 17 with i filter, and LCOGT 1 m/sinistro (CTIO) on 2015 Jul 17 with i filter). Several high resolution spectra were acquired with three spectrographs installed in the ESO La Silla observatory. We obtained 11 spectra using HARPS at the ESO 3.6m telescope, 8 spectra using CORALIE, at the Euler 1.2m telescope and 2 spectra with FEROS at the MPG 2.2m telescope. Table2 provides the light curve data. Radial velocity and bisector span values are presented in Table3 with their corresponding uncertainties. (2 data files).

  18. HAT-P-25b: A Hot-Jupiter Transiting a Moderately Faint G Star

    Science.gov (United States)

    Quinn, S. N.; Bakos, G. Á.; Hartman, J.; Torres, G.; Kovács, G.; Latham, D. W.; Noyes, R. W.; Fischer, D. A.; Johnson, J. A.; Marcy, G. W.; Howard, A. W.; Szentgyorgyi, A.; Fűrész, G.; Buchhave, L. A.; Béky, B.; Sasselov, D. D.; Stefanik, R. P.; Perumpilly, G.; Everett, M.; Lázár, J.; Papp, I.; Sári, P.

    2012-01-01

    We report the discovery of HAT-P-25b, a transiting extrasolar planet orbiting the V = 13.19 G5 dwarf star GSC 1788-01237, with a period P = 3.652836 ± 0.000019 days, transit epoch Tc = 2455176.85173 ± 0.00047 (BJD—barycentric Julian dates throughout the paper are calculated from Coordinated Universal Time, UTC), and transit duration 0.1174 ± 0.0017 days. The host star has a mass of 1.01 ± 0.03 M ⊙, radius of 0.96+0.05 - 0.04 R ⊙, effective temperature 5500 ± 80 K, and metallicity [Fe/H] = +0.31 ± 0.08. The planetary companion has a mass of 0.567 ± 0.022 M J and radius of 1.190+0.081 - 0.056 R J yielding a mean density of 0.42 ± 0.07 g cm-3. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NOAO (A201Hr), NASA (N018Hr and N167Hr), and the NASA Gemini-Keck time-exchange program (G329Hr). Based in part on observations made with the Nordic Optical Telescope, operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway, and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias.

  19. A Progressive Black Top Hat Transformation Algorithm for Estimating Valley Volumes from DEM Data

    Science.gov (United States)

    Luo, W.; Pingel, T.; Heo, J.; Howard, A. D.

    2013-12-01

    The amount of valley incision and valley volume are important parameters in geomorphology and hydrology research, because they are related to the amount erosion (and thus the volume of sediments) and the amount of water needed to create the valley. This is not only the case for terrestrial research but also for planetary research as such figuring out how much water was on Mars. With readily available digital elevation model (DEM) data, the Black Top Hat (BTH) transformation, an image processing technique for extracting dark features on a variable background, has been applied to DEM data to extract valley depth and estimate valley volume. However, previous studies typically use one single structuring element size for extracting the valley feature and one single threshold value for removing noise, resulting in some finer features such as tributaries not being extracted and underestimation of valley volume. Inspired by similar algorithms used in LiDAR data analysis to separate above ground features and bare earth topography, here we propose a progressive BTH (PBTH) transformation algorithm, where the structuring elements size is progressively increased to extract valleys of different orders. In addition, a slope based threshold was introduced to automatically adjust the threshold values for structuring elements with different sizes. Connectivity and shape parameters of the masked regions were used to keep the long linear valleys while removing other smaller non-connected regions. Preliminary application of the PBTH to Grand Canyon and two sites on Mars has produced promising results. More testing and fine-tuning is in progress. The ultimate goal of the project is to apply the algorithm to estimate the volume of valley networks on Mars and the volume of water needed to form the valleys we observe today and thus infer the nature of the hydrologic cycle on early Mars. The project is funded by NASA's Mars Data Analysis program.

  20. A progressive black top hat transformation algorithm for estimating valley volumes on Mars

    Science.gov (United States)

    Luo, Wei; Pingel, Thomas; Heo, Joon; Howard, Alan; Jung, Jaehoon

    2015-02-01

    The depth of valley incision and valley volume are important parameters in understanding the geologic history of early Mars, because they are related to the amount sediments eroded and the quantity of water needed to create the valley networks (VNs). With readily available digital elevation model (DEM) data, the Black Top Hat (BTH) transformation, an image processing technique for extracting dark features on a variable background, has been applied to DEM data to extract valley depth and estimate valley volume. Previous studies typically use a single window size for extracting the valley features and a single threshold value for removing noise, resulting in finer features such as tributaries not being extracted and underestimation of valley volume. Inspired by similar algorithms used in LiDAR data analysis to remove above-ground features to obtain bare-earth topography, here we propose a progressive BTH (PBTH) transformation algorithm, where the window size is progressively increased to extract valleys of different orders. In addition, a slope factor is introduced so that the noise threshold can be automatically adjusted for windows with different sizes. Independently derived VN lines were used to select mask polygons that spatially overlap the VN lines. Volume is calculated as the sum of valley depth within the selected mask multiplied by cell area. Application of the PBTH to a simulated landform (for which the amount of erosion is known) achieved an overall relative accuracy of 96%, in comparison with only 78% for BTH. Application of PBTH to Ma'adim Vallies on Mars not only produced total volume estimates consistent with previous studies, but also revealed the detailed spatial distribution of valley depth. The highly automated PBTH algorithm shows great promise for estimating the volume of VN on Mars on global scale, which is important for understanding its early hydrologic cycle.

  1. Groundwater evolution beneath Hat Yai, a rapidly developing city in Thailand

    Science.gov (United States)

    Lawrence, A. R.; Gooddy, D. C.; Kanatharana, P.; Meesilp, W.; Ramnarong, V.

    2000-09-01

    Many cities and towns in South and Southeast Asia are unsewered, and urban wastewaters are often discharged either directly to the ground or to surface-water canals and channels. This practice can result in widespread contamination of the shallow groundwater. In Hat Yai, southern Thailand, seepage of urban wastewaters has produced substantial deterioration in the quality of the shallow groundwater directly beneath the city. For this reason, the majority of the potable water supply is obtained from groundwater in deeper semi-confined aquifers 30-50 m below the surface. However, downward leakage of shallow groundwater from beneath the city is a significant component of recharge to the deeper aquifer, which has long-term implications for water quality. Results from cored boreholes and shallow nested piezometers are presented. The combination of high organic content of the urban recharge and the shallow depth to the water table has produced strongly reducing conditions in the upper layer and the mobilisation of arsenic. A simple analytical model shows that time scales for downward leakage, from the surface through the upper aquitard to the semi-confined aquifer, are of the order of several decades. Résumé. De nombreuses villes du sud et du sud-est de l'Asie ne possèdent pas de réseaux d'égouts et les eaux usées domestiques s'écoulent souvent directement sur le sol ou dans des canaux et des cours d'eau de surface. Ces pratiques peuvent provoquer une contamination dispersée de la nappe phréatique. A Hat Yai (sud de la Thaïlande), les infiltrations d'eaux usées domestiques sont responsables d'une détérioration notable de la qualité de la nappe phréatique directement sous la ville. Pour cette raison, la majorité de l'eau potable est prélevée dans des aquifères semi-captifs plus profonds, situés entre 30 et 50 m sous la surface. Cependant, une drainance à partir de la nappe phréatique sous la ville constitue une composante significative de la recharge

  2. HAT-P-16b: A 4 M J Planet Transiting a Bright Star on an Eccentric Orbit

    Science.gov (United States)

    Buchhave, L. A.; Bakos, G. Á.; Hartman, J. D.; Torres, G.; Kovács, G.; Latham, D. W.; Noyes, R. W.; Esquerdo, G. A.; Everett, M.; Howard, A. W.; Marcy, G. W.; Fischer, D. A.; Johnson, J. A.; Andersen, J.; Fűrész, G.; Perumpilly, G.; Sasselov, D. D.; Stefanik, R. P.; Béky, B.; Lázár, J.; Papp, I.; Sári, P.

    2010-09-01

    We report the discovery of HAT-P-16b, a transiting extrasolar planet orbiting the V = 10.8 mag F8 dwarf GSC 2792-01700, with a period P = 2.775960 ± 0.000003 days, transit epoch Tc = 2455027.59293 ± 0.00031 (BJD10), and transit duration 0.1276 ± 0.0013 days. The host star has a mass of 1.22 ± 0.04 M sun, radius of 1.24 ± 0.05 R sun, effective temperature 6158 ± 80 K, and metallicity [Fe/H] = +0.17 ± 0.08. The planetary companion has a mass of 4.193 ± 0.094 M J and radius of 1.289 ± 0.066 R J, yielding a mean density of 2.42 ± 0.35 g cm-3. Comparing these observed characteristics with recent theoretical models, we find that HAT-P-16b is consistent with a 1 Gyr H/He-dominated gas giant planet. HAT-P-16b resides in a sparsely populated region of the mass-radius diagram and has a non-zero eccentricity of e = 0.036 with a significance of 10σ. Based in part on observations made with the Nordic Optical Telescope, operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway, and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NASA (N018Hr).

  3. The GTC exoplanet transit spectroscopy survey. IV. Confirmation of the flat transmission spectrum of HAT-P-32b

    Science.gov (United States)

    Nortmann, L.; Pallé, E.; Murgas, F.; Dreizler, S.; Iro, N.; Cabrera-Lavers, A.

    2016-10-01

    We observed the hot Jupiter HAT-P-32b (also known as HAT-P-32Ab) to determine its optical transmission spectrum by measuring the wavelength-dependent, planet-to-star radius ratios in the region between 518-918 nm. We used the OSIRIS instrument at the Gran Telescopio CANARIAS (GTC) in long-slit spectroscopy mode, placing HAT-P-32 and a reference star in the same slit and obtaining a time series of spectra covering two transit events. Using the best quality data set, we were able to yield 20 narrowband transit light curves, with each passband spanning a 20 nm wide interval. After removal of all systematic noise signals and light curve modeling, the uncertainties for the resulting radius ratios lie between 337 and 972 ppm. The radius ratios show little variation with wavelength, suggesting a high altitude cloud layer masking any atmospheric features. Alternatively, a strong depletion in alkali metals or a much smaller than expected planetary atmospheric scale height could be responsible for the lack of atmospheric features. Our result of a flat transmission spectrum is consistent with a previous ground-based study of the optical spectrum of this planet. This agreement between independent results demonstrates that ground-based measurements of exoplanet atmospheres can give reliable and reproducible results despite the fact that the data often is heavily affected by systematic noise as long as the noise source is well understood and properly corrected. We also extract an optical spectrum of the M-dwarf companion HAT-P-32B. Using PHOENIX stellar atmosphere models we determine an effective temperature of Teff = 3187+60-71 K, which is slightly colder than previous studies relying only on broadband infrared data. The 20 narrowband and white light curves are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/594/A65

  4. Convolutional Neural Networks with Batch Normalization for Classifying Hi-hat, Snare, and Bass Percussion Sound Samples

    DEFF Research Database (Denmark)

    Gajhede, Nicolai; Beck, Oliver; Purwins, Hendrik

    2016-01-01

    After having revolutionized image and speech processing, convolu- tional neural networks (CNN) are now starting to become more and more successful in music information retrieval as well. We compare four CNN types for classifying a dataset of more than 3000 acoustic and synthesized samples...... of the most prominent drum set instru- ments (bass, snare, hi-hat). We use the Mel scale log magnitudes (MLS) as a representation for the input of the CNN. We compare the classification results of 1) a CNN (3 conv/max-pool layers and 2 fully connected layers) without drop-out and batch normalization vs. three...

  5. VizieR Online Data Catalog: Sloan i follow-up light curves of HATS-18 (Penev+, 2016)

    Science.gov (United States)

    Penev, K.; Hartman, J. D.; Bakos, G. A.; Ciceri, S.; Brahm, R.; Bayliss, D.; Bento, J.; Jordan, A.; Csubry, Z.; Bhatti, W.; de Val-Borro, M.; Espinoza, N.; Zhou, G.; Mancini, L.; Rabus, M.; Suc, V.; Henning, T.; Schmidt, B.; Noyes, R. W.; Lazar, J.; Papp, I.; Sari, P.

    2017-02-01

    The star HATS-18 was observed by HATSouth instruments between UT 2011 April 18 and UT 2013 July 21 using the HS-2, HS-4, and HS-6 units at the Las Campanas Observatory in Chile, the High Energy Spectroscopic Survey (H.E.S.S.) site in Namibia, and Siding Spring Observatory (SSO) in Australia, respectively. A total of 5372, 3758, and 4008 images of HATS-18 were obtained with HS-2, HS-4, and HS-6, respectively. The observations were obtained through a Sloan r filter with an exposure time of 240s. We obtained follow-up light curves of HATS-18 using the Las Cumbres Observatory Global Telescope (LCOGT) 1m telescope network. An ingress was observed on UT 2015 July 18 with the SBIG camera and a Sloan i filter on the 1m at the South African Astronomical Observatory (SAAO). A total of 33 images were collected at a median cadence of 201s. A full transit was observed on UT 2016 January 22 with the sinistro camera and a Sloan i filter on the 1m at Cerro Tololo Inter-American Observatory. A total of 61 images were collected at a median cadence of 219s. For the record, we also note that a full transit was observed on UT 2016 January 3 with the SBIG camera on the 1m at SAAO; however, due to tracking and weather problems, we were unable to extract high-precision photometry from these images, and therefore do not include these data in our analysis. The data are available in Table1. Spectroscopic follow-up observations of HATS-18 were carried out with WiFeS on the Australian National University (ANU) 2.3m telescope and with the Fiber-fed Extended Range Optical Spectrograph (FEROS) on the MPG 2.2m. A total of three spectra were obtained with WiFeS between UT 2015 February 28 and UT 2015 March 2, two at a resolution of R=Δλ/λ=7000, and one at R=3000. We obtained six R=48000 spectra with FEROS between UT 2015 June 12 and UT 2015 June 20. The data are provided in Table2. (2 data files).

  6. Treatment efficiency in wastewater treatment plant of Hat Yai Municipality by quantitative removal of microbial indicators

    Directory of Open Access Journals (Sweden)

    Duangporn Kantachote

    2009-11-01

    Full Text Available The efficiency of treatment in a wastewater treatment plant of Hat Yai Municipality through stabilization ponds and constructed wetlands was monitored by using the bacterial indicators, total coliforms (TC, fecal coliforms (FC, Escherichia coli and fecal streptococci (FS, and photosynthetic microbes. The sequence of water flow in the wastewater treatment plant is as follows: primary or anaerobic pond (P, facultative pond (F, maturation pond (M, constructed wetlands (W1, W2 and W3, and an effluent storage pond (S for the treated wastewater. The wastewater treatment plant has an approximate area of 3,264,000 m2 (2,040 rai and its dry weather flow was running at only 40,000 m3/ day. There were 10 sampling times used for all the 7 ponds during July-October, 2006.Statistical analysis using a Two-Factorial Design model, indicated that pond types significantly affected temperature, dissolved oxygen (DO, and pH (p<0.05, whereas the time of sampling during the day had a significant effect (p<0.05 only on the temperature and light intensity available to the ponds. There were also significant different removal efficiencies of the different bacterial indicator groups tested (p<0.05. The overall performance of the wastewater treatment plant effectively removed TC, FC, E. coli, and FS as follows, 99.8%, 99.8%, 75.8% and 98.8%, respectively. The amounts of bacterial indicators, except for E. coli, showed a negative correlation with levels of light intensity and DO, whereas there was no correlation between the pH and the different indicator bacteria. There was a positive middle level correlation between pHand chlorophyll a.There were five different divisions of photosynthetic organisms detected throughout the plant as follows, Cyanophyta, Chlorophyta, Bacillariophyta, Euglenophyta, and Pyrrhophyta. The least diversity was found in the anaerobic pond (P as there were only 15 genera. Euglena, an indicator of dirty water, was detected only in this pond. The

  7. Assessment of efficiency of water supply system in Prince of Songkla University, Hat Yai Campus

    Directory of Open Access Journals (Sweden)

    Chaivisit, P.

    2007-03-01

    Full Text Available Assessment of efficiency of water production system in Prince of Songkla University, Hat Yai Campus, was conducted in this study. Topics covered include 1 quality and quantity of raw water, 2 water productionprocess, 3 management and maintenance of water production system, and 4 quality of finished water. Totally, 494 water samples were collected during the study. All water samples were analyzed for turbidity,pH, temperature, conductivity, TDS, total coliforms, fecal coliforms and residual chlorine. In addition to the mentioned parameters, 30 of these water samples were also analyzed for hardness, chloride, nitratenitrogen,sulfate and heavy metals (manganese, copper, zinc, iron, chromium, cadmium, lead and mercury. All water samples were collected during November, 2004 (rainy season and March, 2005 (summer season.It was found that the quality of the water in the Sritrang reservoir fell into Class 2 of Thailand Surface Water Quality Standard that requires ordinary water treatment processes prior to consumptions.Cadmium, lead and mercury were not detected by Inductively Coupled Plasma (ICP method. There are 2 sets of water production system. The first set consists of 4 pressure filters which could reduce turbidity atvarying efficiencies: 33.83%, 18.26%, 42.76% and 65.67% depending mainly on the extent of chemical dosing control and the maintenance of the filter media. Another system employed sedimentation tank andrapid sand filter, which could remove turbidity at 89.28%. When combined finished water from both systems were analyzed, it was found that removals of manganese, copper, zinc, iron and chromium were 42.65%,30.02%, 19.54%, 56.82% and 15.12%, respectively. Residual chlorine concentration of the finished water was not more than 0.2 mg/L and no total coliforms or fecal coliforms were detected. The plant had sufficientand competent personnel to carry out their normal tasks but occasional negligence and lack of specific water testing or

  8. HAT-P-57b: A Short-Period Giant Planet Transiting A Bright Rapidly Rotating A8V Star Confirmed Via Doppler Tomography

    CERN Document Server

    Hartman, J D; Buchhave, L A; Torres, G; Latham, D W; Kovács, G; Bhatti, W; Csubry, Z; de Val-Borro, M; Penev, K; Huang, C X; Béky, B; Bieryla, A; Quinn, S N; Howard, A W; Marcy, G W; Johnson, J A; Isaacson, H; Fischer, D A; Noyes, R W; Falco, E; Esquerdo, G A; Knox, R P; Hinz, P; Lázár, J; Papp, I; Sári, P

    2015-01-01

    We present the discovery of HAT-P-57b, a P = 2.4653 day transiting planet around a V = 10.465 +- 0.029 mag, Teff = 7500 +- 250 K main sequence A8V star with a projected rotation velocity of v sin i = 102.1 +- 1.3 km s^-1. We measure the radius of the planet to be R = 1.413 +- 0.054 R_J and, based on RV observations, place a 95% confidence upper limit on its mass of M < 1.85 M_J . Based on theoretical stellar evolution models, the host star has a mass and radius of 1.47 +- 0.12 M_sun, and 1.500 +- 0.050 R_sun, respectively. Spectroscopic observations made with Keck-I/HIRES during a partial transit event show the Doppler shadow of HAT-P-57b moving across the average spectral line profile of HAT-P- 57, confirming the object as a planetary system. We use these observations, together with analytic formulae that we derive for the line profile distortions, to determine the projected angle between the spin axis of HAT-P-57 and the orbital axis of HAT-P-57b. The data permit two possible solutions, with -16.7 deg &l...

  9. HAT-P-65b and HAT-P-66b: Two Transiting Inflated Hot Jupiters and Observational Evidence for the Re-Inflation of Close-In Giant Planets

    CERN Document Server

    Hartman, Joel D; Bhatti, Waqas; Penev, Kaloyan; Bieryla, Allyson; Latham, David W; Kovács, Géza; Torres, Guillermo; Csubry, Zoltan; de Val-Borro, Miguel; Buchhave, Lars; Kovács, Tamás; Quinn, Samuel; Howard, Andrew W; Isaacson, Howard; Fulton, Benjamin J; Everett, Mark E; Esquerdo, Gilbert A; Béky, Bence; Szklenar, Tamás; Falco, Emilio; Santerne, Alexandre; Boisse, Isabelle; Hébrard, Guillaume; Burrows, Adam; Lázár, Jozsef; Papp, István; Sári, Pál

    2016-01-01

    We present the discovery of the transiting exoplanets HAT-P-65b and HAT-P-66b, with orbital periods of 2.6055 d and 2.9721 d, masses of $0.527 \\pm 0.083$ M$_{J}$ and $0.783 \\pm 0.057$ M$_{J}$ and inflated radii of $1.89 \\pm 0.13$ R$_{J}$ and $1.59^{+0.16}_{-0.10}$ R$_{J}$, respectively. They orbit moderately bright ($V=13.145 \\pm 0.029$, and $V=12.993 \\pm 0.052$) stars of mass $1.212 \\pm 0.050$ M$_{\\odot}$ and $1.255^{+0.107}_{-0.054}$ M$_{\\odot}$. The stars are at the main sequence turnoff. While it is well known that the radii of close-in giant planets are correlated with their equilibrium temperatures, whether or not the radii of planets increase in time as their hosts evolve and become more luminous is an open question. Looking at the broader sample of well-characterized close-in transiting giant planets, we find that there is a statistically significant correlation between planetary radii and the fractional ages of their host stars, with a false alarm probability of only 0.0041%. We find that the correla...

  10. Sulfated small molecules targeting eBV in Burkitt lymphoma: from in silico screening to the evidence of in vitro effect on viral episomal DNA.

    Science.gov (United States)

    Lima, Raquel T; Seca, Hugo; Palmeira, Andreia; Fernandes, Miguel X; Castro, Felipe; Correia-da-Silva, Marta; Nascimento, Maria S J; Sousa, Emília; Pinto, Madalena; Vasconcelos, M Helena

    2013-05-01

    Epstein-Barr virus (EBV) infects more than 90% of the world population. Following primary infection, Epstein-Barr virus persists in an asymptomatic latent state. Occasionally, it may switch to lytic infection. Latent EBV infection has been associated with several diseases, such as Burkitt lymphoma (BL). To date, there are no available drugs to target latent EBV, and the existing broad-spectrum antiviral drugs are mainly active against lytic viral infection. Thus, using computational molecular docking, a virtual screen of a library of small molecules, including xanthones and flavonoids (described with potential for antiviral activity against EBV), was carried out targeting EBV proteins. The more interesting molecules were selected for further computational analysis, and subsequently, the compounds were tested in the Raji (BL) cell line, to evaluate their activity against latent EBV. This work identified three novel sulfated small molecules capable of decreasing EBV levels in a BL. Therefore, the in silico screening presents a good approach for the development of new anti-EBV agents.

  11. HAT-P-56b: A bright highly inflated massive Hot Jupiter around An F star in K2.0 field

    Science.gov (United States)

    Huang, Xu; Bakos, Gaspar; Hartman, Joel

    2015-08-01

    We report the discovery of HAT-P-56b, a transiting high inflated hot-jupiter orbiting a F type star in the field 0 of the NASA K2 mission, by the HATNet survey. We combine ground-based photometric light curves with the highprecision photometry obervation by the K2 mission, as well as radial velocity to determine the physical properties of this system. HAT-P-56b has a mass around ~2.2 Mjunp, a radius of ~1.5 Rjup, and transits its host star with a period of 2.79d. The host star has a V band magnitude of 10.9, Mass of 1.29 Msun, and radius of 1.433 Rsun. The radius of HAT-P-56b is among one of the largest compare to planets with similar mass, making it an interesting target for following up atmospherical observations.

  12. Transmission spectroscopy of HAT-P-32b with the LBT: confirmation of clouds/hazes in the planetary atmosphere

    CERN Document Server

    Mallonn, Matthias

    2016-01-01

    Spectroscopic observations of a transit event of an extrasolar planet offer the opportunity to study the composition of the planetary atmosphere. We observed a transit of the inflated Hot Jupiter HAT-P-32b with MODS at the LBT to characterize its atmosphere from 3300 to 10000 AA. A time series of target and reference star spectra was binned in two broad-band wavelength channels, from which differential transit light curves were constructed. These broad-band light curves were used to confirm previous transit parameter determinations. To derive the planetary transmission spectrum with a resolution of R ~ 60, we created a chromatic set of 62 narrow-band light curves with an average wavelength width of about 100 AA. The spectrum was corrected for the third-light of a near-by M star, whose spectrum was resolved in the individual exposures. Additionally, we undertook a photometric monitoring campaign of the host star to correct for the influence of starspots. The transmission spectrum of HAT-P-32b shows no pressure...

  13. INVESTIGATION OF SYSTEMATIC EFFECTS IN KEPLER DATA: SEASONAL VARIATIONS IN THE LIGHT CURVE OF HAT-P-7b

    Energy Technology Data Exchange (ETDEWEB)

    Van Eylen, V.; Lindholm Nielsen, M.; Hinrup, B.; Tingley, B.; Kjeldsen, H., E-mail: vincent@phys.au.dk [Stellar Astrophysics Centre, Department of Physics and Astronomy, Aarhus University, Ny Munkegade 120, DK-8000 Aarhus C (Denmark)

    2013-09-10

    With years of Kepler data currently available, the measurement of variations in planetary transit depths over time can now be attempted. To do so, it is of primary importance to understand which systematic effects may affect the measurement of transits. We aim to measure the stability of Kepler measurements over years of observations. We present a study of the depth of about 500 transit events of the Hot Jupiter HAT-P-7b, using 14 quarters (Q0-Q13) of data from the Kepler satellite. We find a systematic variation in the depth of the primary transit, related to quarters of data and recurring yearly. These seasonal variations are about 1%. Within seasons, we find no evidence for trends. We speculate that the cause of the seasonal variations could be unknown field crowding or instrumental artifacts. Our results show that care must be taken when combining transits throughout different quarters of Kepler data. Measuring the relative planetary radius of HAT-P-7b without taking these systematic effects into account leads to unrealistically low error estimates. This effect could be present in all Kepler targets. If so, relative radius measurements of all Hot Jupiters to a precision much better than 1% are unrealistic.

  14. A high obliquity orbit for the hot-Jupiter HATS-14b transiting a 5400K star

    CERN Document Server

    Zhou, G; Hartman, J D; Fulton, B J; Bakos, G Á; Howard, A W; Isaacson, H; Marcy, G W; Schmidt, B P; Brahm, R; Jordán, A

    2015-01-01

    We report a spin-orbit misalignment for the hot-Jupiter HATS-14b, measuring a projected orbital obliquity of |lambda|= 76 -5/+4 deg. HATS-14b orbits a high metallicity, 5400 K G dwarf in a relatively short period orbit of 2.8 days. This obliquity was measured via the Rossiter-McLaughlin effect, obtained with observations from Keck-HIRES. The velocities were extracted using a novel technique, optimised for low signal-to-noise spectra, achieving a high precision of 4 m/s point-to-point scatter. However, we caution that our uncertainties may be underestimated. Due to the low rotational velocity of the star, the detection significance is dependent on the vsini prior that is imposed in our modelling. Based on trends observed in the sample of hot Jupiters with obliquity measurements, it has been suggested that these planets modify the spin axes of their host stars, with an efficiency that depends on the stellar type and orbital period of the system. In this framework, short-period planets around stars with surface ...

  15. Relating $\\hat{q}$, $\\eta/s$ and $\\Delta E$ in an expanding Quark-Gluon Plasma

    CERN Document Server

    Ayala, Alejandro; Jalilian-Marian, Jamal; Tejeda-Yeomans, Maria Elena

    2016-01-01

    We use linear viscous hydrodynamics to describe the energy and momentum deposited by a fast moving parton in a quark gluon plasma. This energy-momentum is in turn used to compute the probability density for the production of soft partons by means of the Cooper-Frye formula. We use this probability density to render manifest a relation between the average transverse momentum given to the fast moving parton from the medium $\\hat{q}$, the entropy density to shear viscosity ratio $\\eta/s$ and the energy lost by the fast moving parton $\\Delta E$ in an expanding medium under similar conditions to those generated in nucleus-nucleus collisions at the LHC. We find that $\\hat{q}$ increases linearly with $\\Delta E$ for both trigger and away side partons that have been produced throughout the medium. On the other hand, $\\eta/s$ is more stable with $\\Delta E$. We also study how these transport coefficients vary with the geometrical location of the hard scattering that produces the fast moving partons. The behavior of $\\ha...

  16. Studying the atmosphere of the exoplanet HAT-P-7b via secondary eclipse measurements with EPOXI, Spitzer and Kepler

    CERN Document Server

    Christiansen, Jessie L; Charbonneau, David; Madhusudhan, N; Seager, Sara; Holman, Matthew J; Wellnitz, Dennis D; Deming, Drake; A'Hearn, Michael F

    2009-01-01

    The highly irradiated transiting exoplanet, HAT-P-7b, currently provides one of the best opportunities for studying planetary emission in the optical and infrared wavelengths. We observe six near-consecutive secondary eclipses of HAT-P-7b at optical wavelengths with the EPOXI spacecraft. We place an upper limit on the relative eclipse depth of 0.055% (95% confidence). We also analyze Spitzer observations of the same target in the infrared, obtaining secondary eclipse depths of 0.098+/-0.017%, 0.159+/-0.022%, 0.245+/-0.031% and 0.225+/-0.052% in the 3.6, 4.5, 5.8 and 8.0 micron IRAC bands respectively. We combine these measurements with the recently published Kepler secondary eclipse measurement, and generate atmospheric models for the day-side of the planet that are consistent with both the optical and infrared measurements. The data are best fit by models with a temperature inversion, as expected from the high incident flux. The models predict a low optical albedo of ~< 0.13, with subsolar abundances of N...

  17. A high precision chemical abundance analysis of the HAT-P-1 stellar binary: constraints on planet formation

    CERN Document Server

    Liu, F; Ramirez, I; Yong, D; Melendez, J

    2014-01-01

    We present a high-precision, differential elemental abundance analysis of the HAT-P-1 stellar binary based on high-resolution, high signal-to-noise ratio Keck/HIRES spectra. The secondary star in this double system is known to host a transiting giant planet while no planets have yet been detected around the primary star. The derived metallicities ([Fe/H]) of the primary and secondary stars are identical within the errors: $0.146 \\pm 0.014$ dex ($\\sigma$ = 0.033 dex) and $0.155 \\pm 0.007$ dex ($\\sigma$ = 0.023 dex), respectively. Extremely precise differential abundance ratios of 23 elements have been measured (mean error of $\\sigma$([X/Fe]) = 0.013 dex) and are found to be indistinguishable between the two stars: $\\Delta$[X/Fe] (secondary - primary) = $+0.001 \\pm 0.006$ dex ($\\sigma$ = 0.008 dex). The striking similarity in the chemical composition of the two stellar components in HAT-P-1 is contrary to the possible 0.04 dex level difference seen in 16 Cyg A+B, which also hosts a giant planet, at least 3 time...

  18. A SEARCH FOR WATER IN THE ATMOSPHERE OF HAT-P-26b USING LDSS-3C

    Energy Technology Data Exchange (ETDEWEB)

    Stevenson, Kevin B.; Bean, Jacob L.; Seifahrt, Andreas; Gilbert, Gregory J. [Department of Astronomy and Astrophysics, University of Chicago, 5640 S Ellis Avenue, Chicago, IL 60637 (United States); Line, Michael R. [NASA Ames Research Center, Moffett Field, CA 94035 (United States); Désert, Jean-Michel [Anton Pannekoek Institute for Astronomy, University of Amsterdam, Amsterdam (Netherlands); Fortney, Jonathan J., E-mail: kbs@uchicago.edu [Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States)

    2016-02-01

    The characterization of a physically diverse set of transiting exoplanets is an important and necessary step toward establishing the physical properties linked to the production of obscuring clouds or hazes. It is those planets with identifiable spectroscopic features that can most effectively enhance our understanding of atmospheric chemistry and metallicity. The newly commissioned LDSS-3C instrument on Magellan provides enhanced sensitivity and suppressed fringing in the red optical, thus advancing the search for the spectroscopic signature of water in exoplanetary atmospheres from the ground. Using data acquired by LDSS-3C and the Spitzer Space Telescope, we search for evidence of water vapor in the transmission spectrum of the Neptune-mass planet HAT-P-26b. Our measured spectrum is best explained by the presence of water vapor, a lack of potassium, and either a high-metallicity, cloud-free atmosphere or a solar-metallicity atmosphere with a cloud deck at ∼10 mbar. The emergence of multi-scale-height spectral features in our data suggests that future observations at higher precision could break this degeneracy and reveal the planet’s atmospheric chemical abundances. We also update HAT-P-26b’s transit ephemeris, t{sub 0} = 2455304.65218(25) BJD{sub TDB}, and orbital period, p = 4.2345023(7) days.

  19. HAT-P-26b: A Low-density Neptune-mass Planet Transiting a K Star

    Science.gov (United States)

    Hartman, J. D.; Bakos, G. Á.; Kipping, D. M.; Torres, G.; Kovács, G.; Noyes, R. W.; Latham, D. W.; Howard, A. W.; Fischer, D. A.; Johnson, J. A.; Marcy, G. W.; Isaacson, H.; Quinn, S. N.; Buchhave, L. A.; Béky, B.; Sasselov, D. D.; Stefanik, R. P.; Esquerdo, G. A.; Everett, M.; Perumpilly, G.; Lázár, J.; Papp, I.; Sári, P.

    2011-02-01

    We report the discovery of HAT-P-26b, a transiting extrasolar planet orbiting the moderately bright V = 11.744 K1 dwarf star GSC 0320-01027, with a period P = 4.234516 ± 0.000015 days, transit epoch Tc = 2455304.65122 ± 0.00035 (BJD; Barycentric Julian dates throughout the paper are calculated from Coordinated Universal Time (UTC)), and transit duration 0.1023 ± 0.0010 days. The host star has a mass of 0.82 ± 0.03 M sun, radius of 0.79+0.10 -0.04 R sun, effective temperature 5079 ± 88 K, and metallicity [Fe/H] = -0.04 ± 0.08. The planetary companion has a mass of 0.059 ± 0.007 M J, and radius of 0.565+0.072 -0.032 R J yielding a mean density of 0.40 ± 0.10 g cm-3. HAT-P-26b is the fourth Neptune-mass transiting planet discovered to date. It has a mass that is comparable to those of Neptune and Uranus, and slightly smaller than those of the other transiting Super-Neptunes, but a radius that is ~65% larger than those of Neptune and Uranus, and also larger than those of the other transiting Super-Neptunes. HAT-P-26b is consistent with theoretical models of an irradiated Neptune-mass planet with a 10 M ⊕ heavy element core that comprises gsim50% of its mass with the remainder contained in a significant hydrogen-helium envelope, though the exact composition is uncertain as there are significant differences between various theoretical models at the Neptune-mass regime. The equatorial declination of the star makes it easily accessible to both Northern and Southern ground-based facilities for follow-up observations. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NASA (N018Hr and N167Hr).

  20. Physical properties of the HAT-P-23 and WASP-48 planetary systems from multi-colour photometry

    Science.gov (United States)

    Ciceri, S.; Mancini, L.; Southworth, J.; Bruni, I.; Nikolov, N.; D'Ago, G.; Schröder, T.; Bozza, V.; Tregloan-Reed, J.; Henning, Th.

    2015-05-01

    Context. Accurate and repeated photometric follow-up observations of planetary transit events are important to precisely characterize the physical properties of exoplanets. A good knowledge of the main characteristics of the exoplanets is fundamental in order to trace their origin and evolution. Multi-band photometric observations play an important role in this process. Aims: By using new photometric data, we computed precise estimates of the physical properties of two transiting planetary systems at equilibrium temperatures of ~2000 K. Methods: We present new broadband, multi-colour photometric observations obtained using three small class telescopes and the telescope-defocussing technique. In particular we obtained 11 and 10 light curves covering 8 and 7 transits of HAT-P-23 and WASP-48, respectively. For each of the two targets, one transit event was simultaneously observed through four optical filters. One transit of WASP-48 b was monitored with two telescopes from the same observatory. The physical parameters of the systems were obtained by fitting the transit light curves with jktebop and from published spectroscopic measurements. Results: We have revised the physical parameters of the two planetary systems, finding a smaller radius for both HAT-P-23 b and WASP-48 b, Rb = 1.224 ± 0.037 RJup and Rb = 1.396 ± 0.051 RJup, respectively, than those measured in the discovery papers (Rb = 1.368 ± 0.090 RJup and Rb = 1.67 ± 0.10 RJup). The density of the two planets are higher than those previously published (ρb ~ 1.1 and ~0.3 ρjup for HAT-P-23 and WASP-48, respectively) hence the two hot Jupiters are no longer located in a parameter space region of highly inflated planets. An analysis of the variation of the planet's measured radius as a function of optical wavelength reveals flat transmission spectra within the experimental uncertainties. We also confirm the presence of the eclipsing contact binary NSVS-3071474 in the same field of view of WASP-48, for which

  1. Staatsschutzgesetz: Unkontrollierte Kontrolle und mangelnde Verhältnismäßigkeit. Wer nichts zu verbergen hat, muss trotzdem einiges befürchten

    Directory of Open Access Journals (Sweden)

    Herbert Gnauer

    2015-12-01

    Full Text Available Der Antrag zur Änderung des Staatsschutzgesetzes stößt abermals auf unvermindert heftige Kritik. Herbert Gnauer hat sich intensiv mit dieser Änderung auseinandergesetzt und liefert einen Kommentar zum aktuellen Stand der Dinge.

  2. Is $\\hat{q}$ a physical quantity or just a parameter? and other unanswered questions in High-$p_T$ Physics

    CERN Document Server

    Tannenbaum, M J

    2015-01-01

    The many different theoretical studies of energy loss of a quark or gluon traversing a medium have one thing in common: the transport coefficient of a gluon in the medium, $\\hat{q}$, which is defined as the mean 4-momentum transfer$^2$, $\\left$, by a gluon to the medium per gluon mean free path, $\\lambda_{\\rm mfp}$. In the original BDMPSZ formalism, the energy loss of an outgoing parton, $-dE/dx$, per unit length ($x$) of a medium with total length $L$, due to coherent gluon bremsstrahlung, is proportional to the $\\left$ and takes the form: ${-dE/dx }\\simeq \\alpha_s \\left=\\alpha_s\\, \\mu^2\\, L/\\lambda_{\\rm mfp} =\\alpha_s\\, \\hat{q}\\, L\\ $ , where $\\mu$, is the mean momentum transfer per collision. Thus, the total energy loss in the medium goes like $L^2$. Additionally, the accumulated momentum$^2$, $\\left$, transverse to a gluon traversing a length $L$ in the medium is well approximated by $\\left\\approx\\left=\\hat{q}\\, L$. A simple estimate shows that the $\\left\\approx\\hat{q}\\,L$ should be observable at RHIC at ...

  3. Warm Spitzer and Palomar Near-IR Secondary Eclipse Photometry of Two Hot Jupiters: WASP-48b and HAT-P-23b

    CERN Document Server

    O'Rourke, Joseph G; Zhao, Ming; Fortney, Jonathan J; Burrows, Adam; Agol, Eric; Deming, Drake; Desert, Jean-Michel; Howard, Andrew W; Lewis, Nikole K; Showman, Adam P; Todorov, Kamen O

    2013-01-01

    We report secondary eclipse photometry of two hot Jupiters, WASP-48b and HAT-P-23b, at 3.6 and 4.5 um taken with the InfraRed Array Camera aboard the Spitzer Space Telescope during the warm Spitzer mission and in the H and Ks bands with the Wide Field IR Camera at the Palomar 200-inch Hale Telescope. WASP-48b and HAT-P-23b are Jupiter-mass and twice Jupiter-mass objects orbiting an old, slightly evolved F star and an early G dwarf star, respectively. In the H, Ks, 3.6 um, and 4.5 um bands, respectively, we measure secondary eclipse depths of 0.047% +/- 0.016%, 0.109% +/- 0.027%, 0.176% +/- 0.013%, and 0.214% +/- 0.020% for WASP-48b. In the Ks, 3.6 um, and 4.5 um bands, respectively, we measure secondary eclipse depths of 0.234% +/- 0.046%, 0.248% +/- 0.019%, and 0.309% +/- 0.026% for HAT-P-23b. For WASP-48b and HAT-P-23b, respectively, we measure delays of 2.6 +/- 3.9 minutes and 4.0 +/- 2.4 minutes relative to the predicted times of secondary eclipse for circular orbits, placing 2-sigma upper limits on |e co...

  4. Classical and Mexican hat-type potential energy curve for the hydrogen molecule from a confined Kratzer oscillator

    Science.gov (United States)

    van Hooydonk, G.

    2016-03-01

    We review harmonic oscillator theory for closed, stable quantum systems. The H2 potential energy curve (PEC) of Mexican hat-type, calculated with a confined Kratzer oscillator, is better than the Rydberg-Klein-Rees (RKR) H2 PEC. Compared with QM, the theory of chemical bonding is simplified, since a confined Kratzer oscillator can also lead to the long sought for universal function, once called the Holy Grail of Molecular Spectroscopy. This is validated by reducing PECs for different bonds H2, HF, I2, N2 and O2 to a single one. The equal probability for H2, originating either from HA + HB or HB + HA, is quantified with a Gauss probability density function. At the Bohr scale, a confined harmonic oscillator behaves properly at the extremes of bound two-nucleon quantum systems.

  5. Early-time cosmic dynamics in $f(R)$ and $f(|\\hat\\Omega|)$ extensions of Born-Infeld gravity

    CERN Document Server

    Makarenko, Andrey N; Olmo, Gonzalo J; Rubiera-Garcia, Diego

    2014-01-01

    We consider two types of modifications of Born-Infeld gravity in the Palatini formulation and explore their dynamics in the early universe. One of these families considers $f(R)$ corrections to the Born-Infeld Lagrangian, which can be seen as modifications of the dynamics produced by the quantum effects of matter, while the other consists on different powers of the elementary building block of the Born-Infeld Lagrangian, which we denote by $|\\hat\\Omega|$. We find that the two types of nonsingular solutions that arise in the original Born-Infeld theory are also present in these extensions, being bouncing solutions a stable and robust branch. Singular solutions with a period of approximate de Sitter inflation are found even in universes dominated by radiation.

  6. Importance of energy and angular resolutions in top-hat electrostatic analysers for solar wind proton measurements

    Science.gov (United States)

    De Marco, R.; Marcucci, M. F.; Bruno, R.; D'Amicis, R.; Servidio, S.; Valentini, F.; Lavraud, B.; Louarn, P.; Salatti, M.

    2016-08-01

    We use a numerical code which reproduces the angular/energy response of a typical top-hat electrostatic analyser starting from solar wind proton velocity distribution functions (VDFs) generated by numerical simulations. The simulations are based on the Hybrid Vlasov-Maxwell numerical algorithm which integrates the Vlasov equation for the ion distribution function, while the electrons are treated as a fluid. A virtual satellite launched through the simulation box measures the particle VDFs. Such VDFs are moved from the simulation Cartesian grid to energy-angular coordinates to mimic the response of a real sensor in the solar wind. Different energy-angular resolutions of the analyser are investigated in order to understand the influence of the phase-space resolution in existing and upcoming space missions, with regards to determining the key parameters of plasma dynamics.

  7. Long-Term Transit Timing Monitoring and Refined Light Curve Parameters of HAT-P-13b

    CERN Document Server

    Fulton, Benjamin J; Winn, Joshua N; Holman, Matthew J; Pál, András; Gazak, J Zachary

    2011-01-01

    We present 10 new transit light curves of the transiting hot Jupiter HAT-P-13b, obtained during two observational seasons by three different telescopes. When combined with 12 previously published light curves, we have a sample consisting of 22 transit light curves, spanning 1,041 days across 4 observational seasons. We use this sample to examine the recently observed large-amplitude transit timing variations (P\\'al et al. 2011), and give refined system parameters. We find that the transit times are consistent with a linear ephemeris, with the exception of a single transit time, from UT 2009 Nov 5, for which the measured mid transit time significantly deviates from our linear ephemeris. The nature of this deviation is not clear, and the rest of the data does not show any significant transit timing variation.

  8. A model for the postcollapse equilibrium of cosmological structure: truncated isothermal spheres from top-hat density perturbations

    Science.gov (United States)

    Iliev, I. T.; Shapiro, P. R.; Raga, A. C.

    1998-12-01

    The postcollapse structure of objects which form by gravitational condensation out of the expanding cosmological background universe is a key element in the theory of galaxy formation. Towards this end, we have reconsidered the outcome of the nonlinear growth of a uniform, spherical density perturbation in an unperturbed background universe - the cosmological ``top-hat'' problem. We adopt the usual assumption that the collapse to infinite density at a finite time predicted by the top-hat solution is interrupted by a rapid virialization caused by the growth of small-scale inhomogeneities in the initial perturbation. We replace the standard description of the postcollapse object as a uniform sphere in virial equilibrium by a more self-consistent one as a truncated, nonsingular, isothermal sphere in virial and hydrostatic equilibrium, including for the first time a proper treatment of the finite-pressure boundary condition on the sphere. The results differ significantly from both the uniform sphere and the singular isothermal sphere approximations for the postcollapse objects. These results will have a significant effect on a wide range of applications of the Press-Schechter and other semi-analytical models to cosmology. The truncated isothermal sphere solution presented here predicts the virial temperature and integrated mass distribution of the X-ray clusters formed in the CDM model as found by detailed, 3D, numerical gas and N-body dynamical simulations remarkably well. This solution allows us to derive analytically the numerically-calibrated mass-temperature and radius-temperature scaling laws for X-ray clusters which were derived empirically by Evrard, Metzler and Navarro from simulation results for the CDM model.

  9. Transiting Exoplanet Monitoring Project (TEMP). II. Refined System Parameters and Transit Timing Analysis of HAT-P-33b

    Science.gov (United States)

    Wang, Yong-Hao; Wang, Songhu; Liu, Hui-Gen; Hinse, Tobias C.; Laughlin, Gregory; Wu, Dong-Hong; Zhang, Xiaojia; Zhou, Xu; Wu, Zhenyu; Zhou, Ji-Lin; Wittenmyer, R. A.; Eastman, Jason; Zhang, Hui; Hori, Yasunori; Narita, Norio; Chen, Yuanyuan; Ma, Jun; Peng, Xiyan; Zhang, Tian-Meng; Zou, Hu; Nie, Jun-Dan; Zhou, Zhi-Min

    2017-08-01

    We present 10 R-band photometric observations of eight different transits of the hot Jupiter HAT-P-33b, which has been targeted by our Transiting Exoplanet Monitoring Project. The data were obtained by two telescopes at the Xinglong Station of National Astronomical Observatories of China (NAOC) from 2013 December through 2016 January, and exhibit photometric scatter of 1.6{--}3.0 {mmag}. After jointly analyzing the previously published photometric data, radial-velocity (RV) measurements, and our new light curves, we revisit the system parameters and orbital ephemeris for the HAT-P-33b system. Our results are consistent with the published values except for the planet to star radius ratio ({R}{{P}}/{R}* ), the ingress/egress duration (τ) and the total duration (T 14), which together indicate a slightly shallower and shorter transit shape. Our results are based on more complete light curves, whereas the previously published work had only one complete transit light curve. No significant anomalies in Transit Timing Variations (TTVs) are found, and we place upper mass limits on potential perturbers, largely supplanting the loose constraints provided by the extant RV data. The TTV limits are stronger near mean-motion resonances, especially for the low-order commensurabilities. We can exclude the existence of a perturber with mass larger than 0.6, 0.3, 0.5, 0.5, and 0.3 {M}\\oplus near the 1:3, 1:2, 2:3, 3:2, and 2:1 resonances, respectively.

  10. 31+/-17 ka 40Ar/39Ar Plateau Age on the Very Low-Potassium Hat Creek Basalt Fits Stratigraphic and Geochronologic Constraints of Contiguous Units

    Science.gov (United States)

    Clynne, M. A.; Turrin, B. D.; Champion, D. E.; Muffler, L. P.

    2003-12-01

    The Hat Creek Basalt is a late Pleistocene low-K olivine tholeiite that floors much of the Hat Creek Valley, north of the Lassen volcanic highland in NE California. The flow erupted from a fissure trending N 10° W located 0.5 to 4.0 km SSE of Old Station and flowed north nearly 30 km, mainly through lava tubes. Paleomagnetic data indicate that the Hat Creek Basalt is a single eruptive unit with an inclination of 64.3° and a declination of 1.0° . The Hat Creek Basalt is overlain by gravel related to the last major glaciation, which ended about 15 to 17 ky ago. The Hat Creek Basalt overlies numerous andesite and basaltic andesite lava flows that are closely related geographically, stratigraphically, petrographically and geochemically and probably represent the same eruptive episode (although not necessarily the same year or century). Three of these flows have been dated by the 40Ar/39Ar method and yield concordant plateau, integrated (total fusion), and isochron ages: Little Potato Butte (1.42% K2O; 67+/-4 ka), Sugarloaf (1.85% K2O; 55+/-7 ka), and Big Potato Butte (1.62% K2O; 73+/-14 ka). The Hat Creek Basalt also overlies the basaltic andesite of Cinder Butte (1.65% K2O) with a 40Ar/39Ar plateau age of 38+/-7 ka. Given that the age of the Hat Creek Basalt is thus firmly bracketed between ˜15 ka and 38+/-7 ka, we attempted to date this low-potassium tholeiitic basalt directly. One sample produced 40Ar/39Ar data that cannot be interpreted, but a second sample (0.17% K2O) yielded concordant plateau, integrated (total fusion), and isochron ages of 31+/-17 ka, 30+/-20 ka, and 24+/-6 ka; within the time bracket determined by stratigraphic relations. These data indicate that samples of latest Pleistocene tholeiitic basalt with very low K2O can give useful 40Ar/39Ar ages. As with all isotopically determined ages, the confidence of the results are significantly enhanced when additional constraints imposed by other isotopic ages within a stratigraphic context are taken

  11. Small molecule inhibition of Epstein-Barr virus nuclear antigen-1 DNA binding activity interferes with replication and persistence of the viral genome.

    Science.gov (United States)

    Lee, Eun Kyung; Kim, Sun Young; Noh, Ka-Won; Joo, Eun Hye; Zhao, Bo; Kieff, Elliott; Kang, Myung-Soo

    2014-04-01

    The replication and persistence of extra chromosomal Epstein-Barr virus (EBV) episome in latently infected cells are primarily dependent on the binding of EBV-encoded nuclear antigen 1 (EBNA1) to the cognate EBV oriP element. In continuation of the previous study, herein we characterized EBNA1 small molecule inhibitors (H20, H31) and their underlying inhibitory mechanisms. In silico docking analyses predicted that H20 fits into a pocket in the EBNA1 DNA binding domain (DBD). However, H20 did not significantly affect EBNA1 binding to its cognate sequence. A limited structure-relationship study of H20 identified a hydrophobic compound H31, as an EBNA1 inhibitor. An in vitro EBNA1 EMSA and in vivo EGFP-EBNA1 confocal microscopy analysis showed that H31 inhibited EBNA1-dependent oriP sequence-specific DNA binding activity, but not sequence-nonspecific chromosomal association. Consistent with this, H31 repressed the EBNA1-dependent transcription, replication, and persistence of an EBV oriP plasmid. Furthermore, H31 induced progressive loss of EBV episome. In addition, H31 selectively retarded the growth of EBV-infected LCL or Burkitt's lymphoma cells. These data indicate that H31 inhibition of EBNA1-dependent DNA binding decreases transcription from and persistence of EBV episome in EBV-infected cells. These new compounds might be useful probes for dissecting EBNA1 functions in vitro and in vivo.

  12. High Efficiency White Fluorescent Organic Light-Emitting Diodes with HAT-CN Hole Injection Layer%HAT-CN作为空穴注入层的高效白色荧光有机电致发光二极管

    Institute of Scientific and Technical Information of China (English)

    张静; 张方辉; 阎洪刚

    2011-01-01

    This paper investigated a high efficiency stacked white fluorescent organic lightemitting diodes consisting of separate blue and yellow elements with a 1,4,5,8,9,11-hexaazatriphenylene-hexacar bonitril (HAT-CN) hole injection layer.The configurations of the devices are ITO/2-TNATA or HAT-CN(x nm)/NPB (25 nm)/ADN (30 nm):TBPE (2%):DCJTB(1%)/Alq3(20 nm)/LiF (1 nm)/Al (100 nm).According to the experimental result,the effect of HAT-CN film on carrier injection and color purity were significant.This work obtained a higher efficiency and color purity for the WOLED with HAT-CN layer,as compared to the conventional devices with copper phthalocyanine (CuPc) or 2-TNATA materials as a hole injection layer.It shows pure-white light emitting with 1931 CIE chromaticity coordinates x=0.3309,y=0.3472,and a current efficiency of 6.4 cd/A.%制作了HAT-CN作为空穴注入层的蓝、黄二基色分离的堆叠式高效有机发光二极管,器件结构为:ITO/2-TNATA或HAT-CN(x nm)/NPB(25 nm)/ADN(30 nm):TBPE (2%):DCJTB (1%)/Alq3 (20nm)/LiF(1 nm)/Al(100 nm).实验结果表明,HAT-CN对载流子的注入和色纯度的影响很明显.相比于传统器件将CuPc和2-TNATA作为空穴注入层,HAT-CN作为空穴注入层得到了更高的效率和色纯度,CIE 色坐标x=0.3309,y=0.3472,电流效率达到6.4 cd/A.

  13. En hat er vel en hat ... eller?

    DEFF Research Database (Denmark)

    Bøilerehauge, Dorrit

    2007-01-01

    Mens vi kører i høj fart på "the information highway" - i den helt store bus midt på vejen - glemmer vi ofte noget meget vigtigt i vores glæde over, at verden tilsyneladende åbner sig for os, og vi kan kommunikere med hele verden med ét klik. Vi glemmer, at selvom trafikskilte som oftest er ens...

  14. 3.6 and 4.5 μm Spitzer Phase Curves of the Highly Irradiated Hot Jupiters WASP-19b and HAT-P-7b

    Science.gov (United States)

    Wong, Ian; Knutson, Heather A.; Kataria, Tiffany; Lewis, Nikole K.; Burrows, Adam; Fortney, Jonathan J.; Schwartz, Joel; Shporer, Avi; Agol, Eric; Cowan, Nicolas B.; Deming, Drake; Désert, Jean-Michel; Fulton, Benjamin J.; Howard, Andrew W.; Langton, Jonathan; Laughlin, Gregory; Showman, Adam P.; Todorov, Kamen

    2016-06-01

    We analyze full-orbit phase curve observations of the transiting hot Jupiters WASP-19b and HAT-P-7b at 3.6 and 4.5 μm, obtained using the Spitzer Space Telescope. For WASP-19b, we measure secondary eclipse depths of 0.485%+/- 0.024% and 0.584%+/- 0.029% at 3.6 and 4.5 μm, which are consistent with a single blackbody with effective temperature 2372 ± 60 K. The measured 3.6 and 4.5 μm secondary eclipse depths for HAT-P-7b are 0.156%+/- 0.009% and 0.190%+/- 0.006%, which are well described by a single blackbody with effective temperature 2667 ± 57 K. Comparing the phase curves to the predictions of one-dimensional and three-dimensional atmospheric models, we find that WASP-19b’s dayside emission is consistent with a model atmosphere with no dayside thermal inversion and moderately efficient day-night circulation. We also detect an eastward-shifted hotspot, which suggests the presence of a superrotating equatorial jet. In contrast, HAT-P-7b’s dayside emission suggests a dayside thermal inversion and relatively inefficient day-night circulation; no hotspot shift is detected. For both planets, these same models do not agree with the measured nightside emission. The discrepancies in the model-data comparisons for WASP-19b might be explained by high-altitude silicate clouds on the nightside and/or high atmospheric metallicity, while the very low 3.6 μm nightside planetary brightness for HAT-P-7b may be indicative of an enhanced global C/O ratio. We compute Bond albedos of 0.38 ± 0.06 and 0 (\\lt 0.08 at 1σ ) for WASP-19b and HAT-P-7b, respectively. In the context of other planets with thermal phase curve measurements, we show that WASP-19b and HAT-P-7b fit the general trend of decreasing day-night heat recirculation with increasing irradiation.

  15. Phase II, Title I engineering assessment of inactive uranium mill tailings, Mexican Hat site, Mexican Hat, Utah. [Environmental effects, health hazards, and options for stabilization of tailings and fencing or decontamination of sites

    Energy Technology Data Exchange (ETDEWEB)

    None

    1977-03-31

    An engineering assessment of the problems resulting from the existence of radioactive uranium mill tailings at the Mexican Hat millsite in Utah is presented. Topographic maps, data on core drillings and radiometric measurements sufficient to determine areas and volumes of tailings and other radium-contaminated materials, the evaluation of resulting radiation exposures of individuals residing nearby, the investigation of site hydrology and meteorology, and the evaluation and costing of alternative corrective actions are presented. Radon gas release from the 2,200,000 tons of tailings on the site constitutes the most significant environmental impact. T he six alternative actions presented are directed towards restricting access to the site, returning the windblown tailings to the piles and stabilizing the piles with cover material, and consolidating the two piles into one pile and stabilizing it with cover material. Fencing around the site or the tailings and the decontamination of mill buildings is included in all options. Costs of the options range from $370,000 to $4,390,000.

  16. Phase II, Title I engineering assessment of inactive uranium mill tailings, Mexican Hat site, Mexican Hat, Utah. [Environmental effects, health hazards, and options for stabilization of tailings and fencing or decontamination of sites

    Energy Technology Data Exchange (ETDEWEB)

    None

    1977-03-31

    An engineering assessment of the problems resulting from the existence of radioactive uranium mill tailings at the Mexican Hat millsite in Utah is presented. Topographic maps, data on core drillings and radiometric measurements sufficient to determine areas and volumes of tailings and other radium-contaminated materials, the evaluation of resulting radiation exposures of individuals residing nearby, the investigation of site hydrology and meteorology, and the evaluation and costing of alternative corrective actions are presented. Radon gas release from the 2,200,000 tons of tailings on the site constitutes the most significant environmental impact. T he six alternative actions presented are directed towards restricting access to the site, returning the windblown tailings to the piles and stabilizing the piles with cover material, and consolidating the two piles into one pile and stabilizing it with cover material. Fencing around the site or the tailings and the decontamination of mill buildings is included in all options. Costs of the options range from $370,000 to $4,390,000.

  17. The Black Top Hat function applied to a DEM: A tool to estimate recent incision in a mountainous watershed (Estibère Watershed, Central Pyrenees)

    Science.gov (United States)

    Rodriguez, Felipe; Maire, Eric; Courjault-Radé, Pierre; Darrozes, José

    2002-03-01

    The Top Hat Transform function is a grey-level image analysis tool that allows extracting peaks and valleys in a non-uniform background. This function can be applied onto a grey-level Digital Elevation Model (DEM). It is herein applied to quantify the volume of recent incised material in a mountainous Pyrenean watershed. Grey-level Closing operation applied to the Present-Day DEM gives a new image called ``paleo'' DEM. The Black Top Hat function consists in the subtraction of the ``paleo'' DEM with the Present-Day DEM. It gives a new DEM representing all valleys whose sizes range between the size of the structuring element and the null value as no threshold is used. The calculation of the incised volume is directly derived from the subtraction between the two DEM's. The geological significance of the quantitative results is discussed.

  18. The Transit Light Curve project. XIV. Confirmation of Anomalous Radii for the Exoplanets TrES-4b, HAT-P-3b, and WASP-12b

    CERN Document Server

    Chan, Tucker; Winn, Joshua N; Holman, Matthew J; Sanchis-Ojeda, Roberto; Esquerdo, Gil; Everett, Mark

    2011-01-01

    We present transit photometry of three exoplanets, TrES-4b, HAT-P-3b, and WASP-12b, allowing for refined estimates of the systems' parameters. TrES-4b and WASP-12b were confirmed to be "bloated" planets, with radii of 1.706 +/- 0.056 R_Jup and 1.736 +/- 0.092 R_Jup, respectively. These planets are too large to be explained with standard models of gas giant planets. In contrast, HAT-P-3b has a radius of 0.827 +/- 0.055 R_Jup, smaller than a pure hydrogen-helium planet and indicative of a highly metal-enriched composition. Analyses of the transit timings revealed no significant departures from strict periodicity. For TrES-4, our relatively recent observations allow for improvement in the orbital ephemerides, which is useful for planning future observations.

  19. HAT-P-57b: A Short-period Giant Planet Transiting a Bright Rapidly Rotating A8V Star Confirmed Via Doppler Tomography

    Science.gov (United States)

    Hartman, J. D.; Bakos, G. Á.; Buchhave, L. A.; Torres, G.; Latham, D. W.; Kovács, G.; Bhatti, W.; Csubry, Z.; de Val-Borro, M.; Penev, K.; Huang, C. X.; Béky, B.; Bieryla, A.; Quinn, S. N.; Howard, A. W.; Marcy, G. W.; Johnson, J. A.; Isaacson, H.; Fischer, D. A.; Noyes, R. W.; Falco, E.; Esquerdo, G. A.; Knox, R. P.; Hinz, P.; Lázár, J.; Papp, I.; Sári, P.

    2015-12-01

    We present the discovery of HAT-P-57b, a P = 2.4653 day transiting planet around a V=10.465+/- 0.029 mag, {T}{{eff}}=7500+/- 250 K main sequence A8V star with a projected rotation velocity of v{sin}i=102.1+/- 1.3 {km} {{{s}}}-1. We measure the radius of the planet to be R=1.413+/- 0.054 {R}{{J}} and, based on RV observations, place a 95% confidence upper limit on its mass of M\\lt 1.85 {M}{{J}}. Based on theoretical stellar evolution models, the host star has a mass and radius of 1.47+/- 0.12 {M}⊙ and 1.500+/- 0.050 {R}⊙ , respectively. Spectroscopic observations made with Keck-I/HIRES during a partial transit event show the Doppler shadow of HAT-P-57b moving across the average spectral line profile of HAT-P-57, confirming the object as a planetary system. We use these observations, together with analytic formulae that we derive for the line profile distortions, to determine the projected angle between the spin axis of HAT-P-57 and the orbital axis of HAT-P-57b. The data permit two possible solutions, with -16\\buildrel{\\circ}\\over{.} 7\\lt λ \\lt 3\\buildrel{\\circ}\\over{.} 3 or 27\\buildrel{\\circ}\\over{.} 6\\lt λ \\lt 57\\buildrel{\\circ}\\over{.} 4 at 95% confidence, and with relative probabilities for the two modes of 26% and 74%, respectively. Adaptive optics imaging with MMT/Clio2 reveals an object located 2\\buildrel{\\prime\\prime}\\over{.} 7 from HAT-P-57 consisting of two point sources separated in turn from each other by 0\\buildrel{\\prime\\prime}\\over{.} 22. The H- and {L}\\prime -band magnitudes of the companion stars are consistent with their being physically associated with HAT-P-57, in which case they are stars of mass 0.61+/- 0.10 {M}⊙ and 0.53+/- 0.08 {M}⊙ . HAT-P-57 is the most rapidly rotating star, and only the fourth main sequence A star, known to host a transiting planet. Based on observations obtained with the Hungarian-made Automated Telescope Network. Based in part on observations made with the Keck-I telescope at Mauna

  20. New ${\\cal W}_{q,p}(sl(2))$ algebras from the elliptic algebra ${\\cal A}_{q,p}({\\hat sl}(2)_c)$

    CERN Document Server

    Avan, J; Rossi, M; Sorba, Paul

    1997-01-01

    We construct operators t(z) in the elliptic algebra introduced by Foda et al. ${\\cal A}_{q,p}({\\hat sl}(2)_c)$. They close an exchange algebra when p^m=q^{c+2} for m integer. In addition they commute when p=q^{2k} for k integer non-zero, and they belong to the center of ${\\cal A}_{q,p}({\\hat sl}(2)_c)$ when k is odd. The Poisson structures obtained for t(z) in these classical limits are identical to the q-deformed Virasoro Poisson algebra, characterizing the exchange algebras at generic values of p, q and m as new ${\\cal W}_{q,p}(sl(2))$ algebras.

  1. hat is the Relationship between Entrepreneurship and Unemployment in Visegrad Countries?

    Directory of Open Access Journals (Sweden)

    Ondrej Dvoulety

    2017-06-01

    Full Text Available The presented study aims to quantify the determinants of entrepreneurship in the four countries of the Visegrad (V4 group (the Czech Republic, Hungary, Poland and Slovakia with a particular focus on the relationship between entrepreneurship and unemployment. The purpose of the research was to analyze whether, during the periods of higher unemployment rate, individuals more likely engage into entrepreneurial activity. Data were collected from the national statistical offices of the Visegrad countries, World Bank, Eurostat and Heritage Foundation. The collected sample covered years 1998-2015. To achieve the main objective of the article, regression models with the dependent variable, the rate of registered businesses per economically active inhabitant, were quantified. Estimated regressions proved a positive relationship between entrepreneurship and unemployment. A higher unemployment rate was associated with the increase in overall entrepreneurial activity. Estimated models further confirmed the negative influence of the administrative barriers on the overall entrepreneurial activity. Several policy and research implications are discussed in the study.

  2. Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Mexican Hat, Utah: Appendix D. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1988-07-01

    This appendix is an assessment of the present conditions of the inactive uranium mill site near Mexican Hat, Utah. It consolidates available engineering, radiological, geotechnical, hydrological, meteorological, and other information pertinent to the design of the Remedial Action Plan. Plan is to characterize the conditions at the mill and tailings site so that the Remedial Action Contractor may complete final designs of the remedial action.

  3. Professional little helpers. PHOTON has tested thermography cameras for use in solar sector; Professionelle Helferlein. PHOTON hat Thermografiekameras fuer die Anwendung im Solarbereich getestet

    Energy Technology Data Exchange (ETDEWEB)

    Hinz, Juliane; Kreutzmann, Anne; Siemer, Jochen

    2013-05-15

    The PHOTON Lab has tested again thermography cameras. Eight of the 14 tested cameras reached the ''good'' rating. The three test winner come from two German producers (Testo 885, Testo 876, Flir Systems E60). [German] Das PHOTON-Labor hat erneut Thermographiekameras getestet. Von den 14 getesteten Kameras erreichten acht die Note ''gut''. Die drei Testsieger stammen von zwei deutschen Herstellern (Testo 885, Testo 876, Flir Systems E60).

  4. Single step synthesis of gold-amino acid composite, with the evidence of the catalytic hydrogen atom transfer (HAT) reaction, for the electrochemical recognition of Serotonin

    Science.gov (United States)

    Choudhary, Meenakshi; Siwal, Samarjeet; Nandi, Debkumar; Mallick, Kaushik

    2016-03-01

    A composite architecture of amino acid and gold nanoparticles has been synthesized using a generic route of 'in-situ polymerization and composite formation (IPCF)' [1,2]. The formation mechanism of the composite has been supported by a model hydrogen atom (H•≡H++e-) transfer (HAT) type of reaction which belongs to the proton coupled electron transfer (PCET) mechanism. The 'gold-amino acid composite' was used as a catalyst for the electrochemical recognition of Serotonin.

  5. A case study on a strong tropical disturbance and record heavy rainfall in Hat Yai, Thailand during the winter monsoon

    Science.gov (United States)

    Wangwongchai, Angkool; Zhao, Sixiong; Zeng, Qingcun

    2005-06-01

    The evolutionary process and structural characteristics of the atmospheric circulation and synoptic situation which caused the record heavy rainfall with a precipitation amount of 550 mm in Hat Yai, Thailand from 20 to 23 November 2000 is studied. In the study, the modern three dimensional observational data were collected as completely as possible, and detailed analyses were made. It is revealed that the cold surges of the Asian winter monsoon that originate from Siberia can arrive at the lower latitudes, including South Thailand, Malaysia, Indonesia, cause strong heavy rainfall there, and interact with weather systems in the near-equatorial regions of the Southern Hemisphere. This is strongly supported by Chinese scientist’s original finding in 1930s. The strong convective cloud clusters in the above areas are generated by the direct influence of the cold surges, and are related with the South China Sea disturbances in the lower troposphere. The maximum of the convergence of total moisture flux near South Thailand in the situation under study implies that the water vapour supply is abundant and very favorable to the occurrence of the heavy rainfall. The release of latent heat enhances the Hadley Circulation also. The feedback of the strong severe weather on climate indeed exists, and there are pronounced interactions between the multi-scale systems and between both hemispheres.

  6. A Case Study on a Strong Tropical Disturbance and Record Heavy Rainfall in Hat Yai, Thailand during the Winter Monsoon

    Institute of Scientific and Technical Information of China (English)

    Angkool WANGWONGCHAI; ZHAO Sixiong; ZENG Qingcun

    2005-01-01

    The evolutionary process and structural characteristics of the atmospheric circulation and synoptic situation which caused the record heavy rainfall with a precipitation amount of 550 mm in Hat Yai,Thailand from 20 to 23 November 2000 is studied. In the study, the modern three dimensional observational data were collected as completely as possible, and detailed analyses were made. It is revealed that the cold surges of the Asian winter monsoon that originate from Siberia can arrive at the lower latitudes, including South Thailand, Malaysia, Indonesia, cause strong heavy rainfall there, and interact with weather systems in the near-equatorial regions of the Southern Hemisphere. This is strongly supported by Chinese scientist's original finding in 1930s. The strong convective cloud clusters in the above areas are generated by the direct influence of the cold surges, and are related with the South China Sea disturbances in the lower troposphere. The maximum of the convergence of total moisture flux near South Thailand in the situation under study implies that the water vapour supply is abundant and very favorable to the occurrence of the heavy rainfall. The release of latent heat enhances the Hadley Circulation also. The feedback of the strong severe weather on climate indeed exists, and there are pronounced interactions between the multi-scale systems and between both hemispheres.

  7. Investigation on Twisting and Side Wall Opening Occurring in Curved Hat Channel Products Made of High Strength Steel Sheets

    Science.gov (United States)

    Takamura, Masato; Fukui, Ayako; Yano, Hiroshi; Hama, Takayuki; Sunaga, Hideyuki; Makinouchi, Akitake; Asakawa, Motoo

    2011-08-01

    High strength steel sheets are becoming increasingly important for the weight reduction of automotive bodies to meet the requirements for reduced environmental impact. However, dimensional defects resulting from springback are serious issues, and effective methods of predicting and reducing such defects are necessary. In this study, we numerically and experimentally analyzed the mechanisms of dimensional inaccuracies caused by springback occurring in curved hat channel deep drawing products. The analysis was based on the static explicit FEM software "TP-STRUCT" (the solver part is known as "STAMP3D"). The results of the experiments and simulations similarly show that the twist angle is positive (right-hand system) when the drawing height is relatively large. We calculated the twist torque around the longitudinal axis using the stress distributions obtained by FE analysis. Through the investigation of twist torque and its transition during the drawing and die removal processes, we found that the negative torque generated by side wall opening occurring in the die removal process is the dominant factor of the positive twist. Knowing such mechanisms of twist in cases with a relatively large drawing height, we attempted to explore methods of reducing side wall opening by giving the side wall a stepped shape with the eventual aim of reducing twist. Consequently, we concluded that the stepped shape on the side wall has marked effects of reducing side wall opening, mainly through the elimination of bending-unbending effects on die shoulders, which was verified by observing the stress distribution obtained by FE analysis.

  8. The effects of angiotensinogen gene polymorphisms on cardiovascular disease outcomes during antihypertensive treatment in the GenHAT study

    Directory of Open Access Journals (Sweden)

    Anh N. Do

    2014-09-01

    Full Text Available Previous studies have reported that risk of cardiovascular morbidity and mortality substantially increases in hypertensive patients, especially among those with inadequate blood pressure control. Two common antihypertensive drug classes including thiazide diuretics and angiotensinogen converting enzyme (ACE inhibitors affect different enzymes in the renin angiotensinogen aldosterone system (RAAS. In the RAAS, angiotensinogen is converted into angiotensin II which increases blood pressure through vasoconstriction. Using a case-only design with 3,448 high-risk hypertensive individuals from the Genetics of Hypertension Associated Treatment (GenHAT study, we examined whether 7 single nucleotide polymorphisms (SNPs in the angiotensinogen gene (AGT interact with three classes of antihypertensive drugs including chlorthalidone (a thiazide diuretic, lisinopril (an ACE inhibitor, and amlodipine (a calcium channel blocker to modify the risk of incident coronary heart disease (CHD and heart failure (HF among Caucasian and African American participants, separately. We found no gene by treatment interactions to be statistically significant after correction for multiple testing. However, some suggestive results were found. African American participants with the minor allele of rs11122576 had over two-fold higher risk of CHD when using chlorthalidone compared to using amlodipine, or lisinopril compared to amlodipine (p=0.006, and p=0.01, respectively. Other marginal associations are also reported among both race groups. The findings reported here suggest that rs11122576 could contribute to future personalization of antihypertensive treatment among African Americans though more studies are needed.

  9. Molecular Markers for Identification of Stellantchasmus falcatus and a Phylogenic Study using the HAT-RAPD Method

    Science.gov (United States)

    Wongsawad, Pheravut

    2010-01-01

    Stellantchasmus falcatus is a minute intestinal fluke in the family Heterophyidae. Metacercariae, the infective stage, were reported in a marine fish, mullet Liza subviridis, and a fresh water fish, Dermogenus pusillus, in Thailand. Adults were found in chicks, rats, cats, and humans. Morphological studies were done for comparing Stellantchasmus sp. worms found in 2 different fish hosts; their shapes and organ arrangements were very similar except for the prepharynx length. Therefore, the present study aimed to compare their DNA fingerprints using the HAT-RAPD method for both types of Stellantchasmus and several other related species. Ten arbitrarily selected primers (OPA-04, OPA-09, OPN-02, OPN-03, OPN-09, OPN-12, OPP-11, OPR-15, OPX-13, and OPAD-01) were used. It was found that OPA-09, OPN-03, and OPAD-01 were able to generate S. falcatus specific fragments in mullets which consisted of 200, 760, and 280 bp, respectively. In addition, the results of morphologic, DNA fingerprinting, and phylogenetic analyses strongly suggest that the fresh water and marine specimens of Stellantchamus may be different species. PMID:21234232

  10. The HAT-P-13 Exoplanetary System: Evidence for Spin-Orbit Alignment and a Third Companion

    CERN Document Server

    Winn, Joshua N; Howard, Andrew W; Marcy, Geoffrey W; Bakos, Gaspar A; Hartman, Joel; Torres, Guillermo; Albrecht, Simon; Narita, Norio

    2010-01-01

    We present new radial-velocity measurements of HAT-P-13, a star with two previously known companions: a transiting giant planet "b" with an orbital period of 3 days, and a more massive object "c" on a 1.2 yr, highly eccentric orbit. For this system, dynamical considerations would lead to constraints on planet b's interior structure, if it could be shown that the orbits are coplanar and apsidally locked. By modeling the Rossiter-McLaughlin effect, we show that planet b's orbital angular momentum vector and the stellar spin vector are well-aligned on the sky (lambda = -0.9 +/- 8.5 deg), suggesting that the planetary orbits are also well-aligned. The refined orbital solution favors a slightly eccentric orbit for planet b (e = 0.0142_{-0.0044}^{+0.0052}), although it is not clear whether it is apsidally locked with c's orbit (\\Delta\\omega = 48_{-38}^{+25} deg). We find a long-term trend in the star's radial velocity and interpret it as evidence for an additional body "d", which may be another planet or a low-mass...

  11. Peak center and area estimation in gamma-ray energy spectra using a Mexican-hat wavelet

    Science.gov (United States)

    Qin, Zhang-jian; Chen, Chuan; Luo, Jun-song; Xie, Xing-hong; Ge, Liang-quan; Wu, Qi-fan

    2017-06-01

    Wavelet analysis is commonly used to detect and localize peaks within a signal, such as in Gamma-ray energy spectra. This paper presents a peak area estimation method based on a new wavelet analysis. Another Mexican Hat Wavelet Signal (MHWS) named after the new MHWS is obtained with the convolution of a Gaussian signal and a MHWS. During the transform, the overlapping background on the Gaussian signal caused by Compton scattering can be subtracted because the impulse response function MHWS is a second-order smooth function, and the amplitude of the maximum within the new MHWS is the net height corresponding to the Gaussian signal height, which can be used to estimate the Gaussian peak area. Moreover, the zero-crossing points within the new MHWS contain the information of the Gaussian variance whose valve should be obtained when the Gaussian peak area is estimated. Further, the new MHWS center is also the Gaussian peak center. With that distinguishing feature, the channel address of a characteristic peak center can be accurately obtained which is very useful in the stabilization of airborne Gamma energy spectra. In particular, a method for determining the correction coefficient k is given, where the peak area is calculated inaccurately because the value of the scale factor in wavelet transform is too small. The simulation and practical applications show the feasibility of the proposed peak center and area estimation method.

  12. GTC OSIRIS transiting exoplanet atmospheric survey: detection of potassium in HAT-P-1b from narrowband spectrophotometry

    CERN Document Server

    Wilson, P A; Nikolov, N; Etangs, A Lecavelier des; Pont, F; Fortney, J J; Ballester, G E; López-Morales, M; Désert, J -M; Vidal-Madjar, A

    2015-01-01

    We present the detection of potassium in the atmosphere of HAT-P-1b using optical transit narrowband photometry. The results are obtained using the 10.4 m Gran Telescopio Canarias (GTC) together with the OSIRIS instrument in tunable filter imaging mode. We observed four transits, two at continuum wavelengths outside the potassium feature, at 6792 {\\AA} and 8844 {\\AA}, and two probing the potassium feature in the line wing at 7582.0 {\\AA} and the line core at 7664.9 {\\AA} using a 12 {\\AA} filter width (R~650). The planet-to-star radius ratios in the continuum are found to be $R_{\\rm{pl}}/R_{\\star}$ = 0.1176 $\\pm$ 0.0013 at 6792 {\\AA} and $R_{\\rm{pl}}/R_{\\star}$ = 0.1168 $\\pm$ 0.0022 at 8844 {\\AA}, significantly lower than the two observations in the potassium line: $R_{\\rm{pl}}/R_{\\star}$ = 0.1248 $\\pm$ 0.0014 in the line wing at 7582.0 {\\AA} and $R_{\\rm{pl}}/R_{\\star}$ = 0.1268 $\\pm$ 0.0012 in the line core at 7664.9 {\\AA}. With a weighted mean of the observations outside the potassium feature $R_{\\rm{pl}}/R_...

  13. Comparative analysis of the recently discovered hAT transposon TcBuster in human cells.

    Directory of Open Access Journals (Sweden)

    Lauren E Woodard

    Full Text Available BACKGROUND: Transposons are useful tools for creating transgenic organisms, insertional mutagenesis, and genome engineering. TcBuster, a novel hAT-family transposon system derived from the red flour beetle Tribolium castaneum, was shown to be highly active in previous studies in insect embryoes. METHODOLOGY/PRINCIPAL FINDINGS: We tested TcBuster for its activity in human embryonic kidney 293 (HEK-293 cells. Excision footprints obtained from HEK-293 cells contained small insertions and deletions consistent with a hAT-type repair mechanism of hairpin formation and non-homologous end-joining. Genome-wide analysis of 23,417 piggyBac, 30,303 Sleeping Beauty, and 27,985 TcBuster integrations in HEK-293 cells revealed a uniquely different integration pattern when compared to other transposon systems with regards to genomic elements. TcBuster experimental conditions were optimized to assay TcBuster activity in HEK-293 cells by colony assay selection for a neomycin-containing transposon. Increasing transposon plasmid increased the number of colonies, whereas gene transfer activity dependent on codon-optimized transposase plasmid peaked at 100 ng with decreased colonies at the highest doses of transposase DNA. Expression of the related human proteins Buster1, Buster3, and SCAND3 in HEK-293 cells did not result in genomic integration of the TcBuster transposon. TcBuster, Tol2, and piggyBac were compared directly at different ratios of transposon to transposase and found to be approximately comparable while having their own ratio preferences. CONCLUSIONS/SIGNIFICANCE: TcBuster was found to be highly active in mammalian HEK-293 cells and represents a promising tool for mammalian genome engineering.

  14. The application of the Mid-IR radio correlation to the $\\hat{G}$ sample and the search for advanced extraterrestrial civilisations

    CERN Document Server

    Garrett, Michael

    2015-01-01

    Wright et al. 2014 have embarked on a search for advanced Karadashev Type III civilisations via the compilation of a sample of sources with extreme mid-IR emission and colours. In this scenario, the mid-IR emission is then primarily associated with waste heat energy by-products. I apply the Mid-IR radio correlation to this $\\hat{G}$ sample (Griffith et al. 2015). I demonstrate that the mid-IR and radio luminosities are correlated for the sample with $q_{22}=1.35\\pm0.42 $. By comparison, the First Look Survey (FLS) has $q_{22}=0.87\\pm0.27$. The fact that the G-HAT sample largely follows the Mid-IR radio correlation, strongly suggests the vast majority of these sources are associated with galaxies in which natural astrophysical processes are dominant. This simple application of the mid-IR radio correlation can substantially reduce the number of false positives in the $\\hat{G}$ catalogue, since galaxies occupied by advanced Kardashev Type III civilisations would be expected to exhibit very high values of $q$. In...

  15. HATS-6b: A Warm Saturn Transiting an Early M Dwarf Star, and a Set of Empirical Relations for Characterizing K and M Dwarf Planet Hosts

    CERN Document Server

    Hartman, J D; Brahm, R; Bakos, G Á; Mancini, L; Jordán, A; Penev, K; Rabus, M; Zhou, G; Butler, R P; Espinoza, N; de Val-Borro, M; Bhatti, W; Csubry, Z; Ciceri, S; Henning, T; Schmidt, B; Arriagada, P; Shectman, S; Crane, J; Thompson, I; Suc, V; Csák, B; Tan, T G; Noyes, R W; Lázár, J; Papp, I; Sári, P

    2014-01-01

    We report the discovery by the HATSouth survey of HATS-6b, an extrasolar planet transiting a V=15.2 mag, i=13.7 mag M1V star with a mass of 0.57 Msun and a radius of 0.57 Rsun. HATS-6b has a period of P = 3.3253 d, mass of Mp=0.32 Mjup, radius of Rp=1.00 Rjup, and zero-albedo equilibrium temperature of Teq=712.8+-5.1 K. HATS-6 is one of the lowest mass stars known to host a close-in gas giant planet, and its transits are among the deepest of any known transiting planet system. We discuss the follow-up opportunities afforded by this system, noting that despite the faintness of the host star, it is expected to have the highest K-band S/N transmission spectrum among known gas giant planets with Teq < 750 K. In order to characterize the star we present a new set of empirical relations between the density, radius, mass, bolometric magnitude, and V, J, H and K-band bolometric corrections for main sequence stars with M < 0.80 Msun, or spectral types later than K5. These relations are calibrated using eclipsing...

  16. HAT-P-24b: An inflated hot-Jupiter on a 3.36d period transiting a hot, metal-poor star

    CERN Document Server

    Kipping, D M; Hartman, J; Torres, G; Shporer, A; Latham, D W; Kovacs, Geza; Noyes, R W; Howard, A W; Fischer, D A; Johnson, J A; Marcy, G W; Beky, B; Perumpilly, G; Esquerdo, G A; Sasselov, D D; Stefanik, R P; Lazar, J; Papp, I; Sari, P

    2010-01-01

    We report the discovery of HAT-P-24b, a transiting extrasolar planet orbiting the moderately bright V=11.818 F8 dwarf star GSC 0774-01441, with a period P = 3.3552464 +/- 0.0000071 d, transit epoch Tc = 2455216.97669 +/- 0.00024 (BJD_UTC), and transit duration 3.653 +/- 0.025 hours. The host star has a mass of 1.186 +/- 0.042 Msun , radius of 1.294 +/- 0.071 Rsun , effective temperature 6373 +/- 80 K, and a low metallicity of [Fe/H] = -0.16 +/- 0.08. The planetary companion has a mass of 0.681 +/- 0.031 MJ , and radius of 1.243 +/- 0.072 RJ yielding a mean density of 0.439 +/- 0.069 g cm-3 . By repeating our global fits with different parameter sets, we have performed a critical investigation of the fitting techniques used for previous HAT planetary discoveries. We find that the system properties are robust against the choice of priors. The effects of fixed versus fitted limb darkening are also examined. HAT-P-24b probably maintains a small eccentricity of e = 0.052 +0.022 -0.017, which is accepted over the c...

  17. Transmission spectroscopy of HAT-P-32b with the LBT: confirmation of clouds/hazes in the planetary atmosphere

    Science.gov (United States)

    Mallonn, M.; Strassmeier, K. G.

    2016-05-01

    Aims: Spectroscopic observations of a transit event of an extrasolar planet offer the opportunity to study the composition of the planetary atmosphere. This can be done with comparably little telescope time using a low-resolution multi-object spectrograph at a large aperture telescope. We observed a transit of the inflated hot Jupiter HAT-P-32b with the Multi-Object Double Spectrograph at the Large Binocular Telescope to characterize its atmosphere from 3300 to 10 000 Å. Methods: A time series of target and reference star spectra was binned in two broad-band wavelength channels, from which differential transit light curves were constructed. These broad-band light curves were used to confirm previous transit parameter determinations. To derive the planetary transmission spectrum with a resolution of R ~ 60, we created a chromatic set of 62 narrow-band light curves. The spectrum was corrected for the third light of a nearby M star. Additionally, we undertook a photometric monitoring campaign of the host star to correct for the influence of starspots. Results: The transmission spectrum of HAT-P-32b shows no pressure-broadened absorption features from Na and K, which is interpreted by the presence of clouds or hazes in the planetary atmosphere. This result is in agreement with previous studies on the same planet. The presence of TiO in gas phase could be ruled out. We find a 2.8σ indication of increased absorption in the line core of potassium (K I 7699 Å). No narrow absorption features of Na and Hα were detected. Furthermore, tentative indications were found for a slope of increasing opacity toward blue wavelengths from the near-IR to the near-UV with an amplitude of two scale heights. If confirmed by follow-up observations, it can be explained by aerosols either causing Mie scattering or causing Rayleigh scattering with an aerosol - gas scale height ratio below unity. The host star was found to be photometrically stable within the measurement precision. Based on

  18. Evaluation of histone deacetylase inhibitors (HDACi) as therapeutic leads for human African trypanosomiasis (HAT).

    Science.gov (United States)

    Carrillo, Angela K; Guiguemde, W Armand; Guy, R Kiplin

    2015-08-15

    Two of the histone deacetylases, TbDAC1 and TbDAC3, have been reported to be essential genes in trypanosomes. Therefore, we tested the activity of a panel of human histone deacetylase inhibitors (HDACi) for their ability to block proliferation of Trypanosoma brucei brucei. Among the HDACi's, the hydroxamic acid derivatives panobinostat and belinostat exhibited potency that appeared to make them viable candidates for development due to their reported pharmacokinetic characteristics. However, cellular pharmacodynamic analysis demonstrated that these drugs were unable to kill cultured parasites at exposures seen in patients at their tolerated doses and additionally failed to show any synergistic effects in combination with pentamidine, suramin, melarsoprol, or nifurtimox. Analysis of the potency of the entire HDACi panel revealed no correlations between potency against any human HDAC isoform and inhibition of T. brucei proliferation, suggesting that the trypanosome histone deacetylases possess a unique specificity. These studies confirmed that HDAC inhibitors have potential as leads against human African trypanosomiasis but that none of the current clinical candidates can be directly repurposed. Therefore, development of HDACi's with appropriate specificity and potency may be a viable route to a new class of anti-trypanosomal drugs.

  19. THE STELLAR OBLIQUITY AND THE LONG-PERIOD PLANET IN THE HAT-P-17 EXOPLANETARY SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    Fulton, Benjamin J.; Howard, Andrew W. [Institute for Astronomy, University of Hawaii at Manoa, 2680 Woodlawn Dr, Honolulu, HI 96822 (United States); Winn, Joshua N.; Albrecht, Simon [Department of Physics and Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Marcy, Geoffrey W.; Isaacson, Howard [University of California, Berkeley, Berkeley, CA (United States); Crepp, Justin R. [University of Notre Dame, Department of Physics, Notre Dame, IN (United States); Bakos, Gaspar A.; Hartman, Joel D. [Princeton University, Department of Astrophysical Sciences, Princeton, NJ (United States); Johnson, John Asher; Knutson, Heather A. [California Institute of Technology, Pasadena, CA (United States); Zhao Ming [Department of Astronomy and Astrophysics, 525 Davey Laboratory, Pennsylvania State University, University Park, PA 16802 (United States)

    2013-08-01

    We present the measured projected obliquity-the sky-projected angle between the stellar spin axis and orbital angular momentum-of the inner planet of the HAT-P-17 multi-planet system. We measure the sky-projected obliquity of the star to be {lambda}=19{sup +14}{sub -16} deg by modeling the Rossiter-McLaughlin effect in Keck/HIRES radial velocities (RVs). The anomalous RV time series shows an asymmetry relative to the midtransit time, ordinarily suggesting a nonzero obliquity-but in this case at least part of the asymmetry may be due to the convective blueshift, increasing the uncertainty in the determination of {lambda}. We employ the semi-analytical approach of Hirano et al. that includes the effects of macroturbulence, instrumental broadening, and convective blueshift to accurately model the anomaly in the net RV caused by the planet eclipsing part of the rotating star. Obliquity measurements are an important tool for testing theories of planet formation and migration. To date, the measured obliquities of {approx}50 Jovian planets span the full range, from prograde to retrograde, with planets orbiting cool stars preferentially showing alignment of stellar spins and planetary orbits. Our results are consistent with this pattern emerging from tidal interactions in the convective envelopes of cool stars and close-in planets. In addition, our 1.8 yr of new RVs for this system show that the orbit of the outer planet is more poorly constrained than previously thought, with an orbital period now in the range of 10-36 yr.

  20. Classification of Ultra-High Resolution Orthophotos Combined with DSM Using a Dual Morphological Top Hat Profile

    Directory of Open Access Journals (Sweden)

    Qian Zhang

    2015-12-01

    Full Text Available New aerial sensors and platforms (e.g., unmanned aerial vehicles (UAVs are capable of providing ultra-high resolution remote sensing data (less than a 30-cm ground sampling distance (GSD. This type of data is an important source for interpreting sub-building level objects; however, it has not yet been explored. The large-scale differences of urban objects, the high spectral variability and the large perspective effect bring difficulties to the design of descriptive features. Therefore, features representing the spatial information of the objects are essential for dealing with the spectral ambiguity. In this paper, we proposed a dual morphology top-hat profile (DMTHP using both morphology reconstruction and erosion with different granularities. Due to the high dimensional feature space, we have proposed an adaptive scale selection procedure to reduce the feature dimension according to the training samples. The DMTHP is extracted from both images and Digital Surface Models (DSM to obtain complimentary information. The random forest classifier is used to classify the features hierarchically. Quantitative experimental results on aerial images with 9-cm and UAV images with 5-cm GSD are performed. Under our experiments, improvements of 10% and 2% in overall accuracy are obtained in comparison with the well-known differential morphological profile (DMP feature, and superior performance is observed over other tested features. Large format data with 20,000 × 20,000 pixels are used to perform a qualitative experiment using the proposed method, which shows its promising potential. The experiments also demonstrate that the DSM information has greatly enhanced the classification accuracy. In the best case in our experiment, it gives rise to a classification accuracy from 63.93% (spectral information only to 94.48% (the proposed method.

  1. Wave-turbulence description of interacting particles: Klein-Gordon model with a Mexican-hat potential.

    Science.gov (United States)

    Gallet, Basile; Nazarenko, Sergey; Dubrulle, Bérengère

    2015-07-01

    In field theory, particles are waves or excitations that propagate on the fundamental state. In experiments or cosmological models, one typically wants to compute the out-of-equilibrium evolution of a given initial distribution of such waves. Wave turbulence deals with out-of-equilibrium ensembles of weakly nonlinear waves, and is therefore well suited to address this problem. As an example, we consider the complex Klein-Gordon equation with a Mexican-hat potential. This simple equation displays two kinds of excitations around the fundamental state: massive particles and massless Goldstone bosons. The former are waves with a nonzero frequency for vanishing wave number, whereas the latter obey an acoustic dispersion relation. Using wave-turbulence theory, we derive wave kinetic equations that govern the coupled evolution of the spectra of massive and massless waves. We first consider the thermodynamic solutions to these equations and study the wave condensation transition, which is the classical equivalent of Bose-Einstein condensation. We then focus on nonlocal interactions in wave-number space: we study the decay of an ensemble of massive particles into massless ones. Under rather general conditions, these massless particles accumulate at low wave number. We study the dynamics of waves coexisting with such a strong condensate, and we compute rigorously a nonlocal Kolmogorov-Zakharov solution, where particles are transferred nonlocally to the condensate, while energy cascades towards large wave numbers through local interactions. This nonlocal cascading state constitutes the intermediate asymptotics between the initial distribution of waves and the thermodynamic state reached in the long-time limit.

  2. Wave-turbulence description of interacting particles: Klein-Gordon model with a Mexican-hat potential

    Science.gov (United States)

    Gallet, Basile; Nazarenko, Sergey; Dubrulle, Bérengère

    2015-07-01

    In field theory, particles are waves or excitations that propagate on the fundamental state. In experiments or cosmological models, one typically wants to compute the out-of-equilibrium evolution of a given initial distribution of such waves. Wave turbulence deals with out-of-equilibrium ensembles of weakly nonlinear waves, and is therefore well suited to address this problem. As an example, we consider the complex Klein-Gordon equation with a Mexican-hat potential. This simple equation displays two kinds of excitations around the fundamental state: massive particles and massless Goldstone bosons. The former are waves with a nonzero frequency for vanishing wave number, whereas the latter obey an acoustic dispersion relation. Using wave-turbulence theory, we derive wave kinetic equations that govern the coupled evolution of the spectra of massive and massless waves. We first consider the thermodynamic solutions to these equations and study the wave condensation transition, which is the classical equivalent of Bose-Einstein condensation. We then focus on nonlocal interactions in wave-number space: we study the decay of an ensemble of massive particles into massless ones. Under rather general conditions, these massless particles accumulate at low wave number. We study the dynamics of waves coexisting with such a strong condensate, and we compute rigorously a nonlocal Kolmogorov-Zakharov solution, where particles are transferred nonlocally to the condensate, while energy cascades towards large wave numbers through local interactions. This nonlocal cascading state constitutes the intermediate asymptotics between the initial distribution of waves and the thermodynamic state reached in the long-time limit.

  3. Characterization and biological treatment of colored textile wastewaters from the typical Tunisian hat Chechia dyeing using newly isolated Aspergillus niger

    Directory of Open Access Journals (Sweden)

    Hajer Barouni

    2016-09-01

    Full Text Available This study aimed to characterize and investigate, for the first time, the treatment of real colored wastewaters from the artisanal dyeing of the typical Tunisian hat Chechia, using a newly isolated fungal strain. This textile effluent was a mixture called Mix of colored wastewaters from the three main types of Chechia. The major pollutant of the Mix was the toxic Azo dye Amaranth Acid or Acid Red 27. The fungal strain that made the cleanup was discovered in a Chechia dyeing wastewater’s container and identified by ITS rDNA gene sequencing. This isolated Aspergillus niger showed interesting performances on the demonstration of Chechia wastewater’s biodegradation in batch cultures. In order to understand the effect of agitation, Mix dilution and inoculum size on decolourisation and pollution removal, a full factorial experimental design 23 was set up. At the optimal conditions which were 20% inoculum size, 25% Chechia Mix dilution and an agitation of 100 rpm, Aspergillus niger was able to remove color as high as 70.18±2.84% at an initial dye concentration of 1346.6±0.01 mg/L, and to reduce COD to 74.17±14.52% at an initial COD of 4157±422 mg/L. FT-IR spectra analysis confirmed the decolourisation by biodegradation and transformation of the dyes. The treatment by the isolated Aspergillus niger could be successfully applied as a sustainable method to solve one of handicraft dyeing plants environmental management issues.

  4. 什么是公民教育%W hat is the Civic Education

    Institute of Scientific and Technical Information of China (English)

    韩益凤

    2014-01-01

    In practice , the civic education w hich is to cultivate citizens , bring up citizenship , and to shape civic virtues will be affected by the changes of some related concepts as citizens 、citizenship and so on . At the same time , the civic education as a kind of special educational activity , naturally , will change with the development of the education thoughts and ideas . On the one hand ,civic education is just a way to make good citizens ;on the other hand , civic education is just one aspect of education functions . However ,in any case , citizens are not only need to be educated , but also can be educated . So the ultimate ideal of the civic education inevitably point to the human good life .%实践中,以培养公民、造就公民身份、塑造公民道德为指向的公民教育不可避免地会受到公民、公民身份等相关概念内涵变化的影响。同时,公民教育作为一种特殊的教育活动,自然也会伴随教育思想、教育理念的发展变化而变化。一方面公民教育只是造就公民的一种途径,另一方面公民教育也只是教育功能的一个方面。不过,无论如何,公民都不但需要教育,而且可以教育。公民教育的终极理想必然指向人类的善好生活。

  5. Micromamíferos del Pleistoceno Medio y Pleistoceno Superior en el Valle del Jarama: yacimientos de Valdocarros y HAT (Madrid, España

    Directory of Open Access Journals (Sweden)

    Sesé, C.

    2011-06-01

    Full Text Available We study the micromammals from the sites of Valdocarros and HAT, located in the lithostratigraphic unit of Arganda II and IV respectively, within the Arganda Complex Terrace, in the Jarama Valley (Madrid, Spain. The micromammal associations of these sites consist of the following taxa: in Valdocarros: Erinaceomorpha: Erinaceus europaeus; Soricomorpha: Crocidura aff. russula; Rodentia: Eliomys quercinus, Castor fiber, Apodemus sp., Cricetulus (Allocricetus bursae, Arvicola aff. sapidus and Microtus brecciensis; and Lagomorpha: Oryctolagus cuniculus; and in HAT: Soricomorpha: Soricidae indet.; Rodentia: Eliomys quercinus, Apodemus sp., Arvicola aff. sapidus and Microtus cf. cabrerae; and Lagomorpha: cf. Oryctolagus sp. The micromammal assemblage of the Valdocarros site can be placed in the advanced Middle Pleistocene, but not in its end, because of the faunal association and the evolved state of Microtus brecciensis. However, in HAT, the presence of Microtus cf. cabrerae suggests that this site belongs to the Late Pleistocene. Both micromammal assemblages indicate a temperate climate and a well developed vegetation cover, consisting of some forest and also open areas, with shrub-like vegetation and also meadow and riverside vegetation.

    Se realiza el estudio de los micromamíferos del yacimiento de Valdocarros, que se localiza en la uni dad de Arganda II de la Terraza Compleja de Arganda, en el valle del Jarama (Madrid, y del yacimiento de HAT, que está en la unidad de Arganda IV. Las asociaciones de micromamíferos determinadas en este trabajo en dichos yacimientos son las siguientes: en Valdocarros: erinaceomorfos: Erinaceus europaeus; soricomorfos: Crocidura aff. russula; roedores: Eliomys quercinus, Castor fiber, Apodemus sp., Cricetulus (Allocricetus bursae, Arvicola aff. sapidus y Microtus brecciensis; y lagomorfos:

  6. Industrial mJ-class all-fiber front end with spatially coherent top-hat beam output used as seeder for high power laser

    Science.gov (United States)

    Gleyze, Jean-François; Perrin, A.; Calvet, Pierre; Gouriou, Pierre; Scol, Florent; Valentin, Constance; Bouwmans, Géraud; Lecren, E.; Hugonnot, Emmanuel

    2015-05-01

    In large scale laser facility dedicated to laser-matter interaction including inertial confinement fusion, such as LMJ or NIF, high-energy main amplifier is injected by a laser source in which the beam parameters must be controlled. For many years, the CEA has developed nano-joule pulses all-fiber front end sources, based on the telecommunications fiber optics technologies. Thanks to these technologies, we have been able to precisely control temporal shaping and phase-modulated pulse. Nowadays, fiber lasers are able to deliver very high power beams and high energy pulses for industrial needs (laser marking, welding,…). Therefore, we have currently developed new nanosecond pulses fibered amplifiers able to increase output pulse energy up to the mJ level. These amplifiers are based on flexible fibers and not on rod type. This allows us to achieve a compact source. Nevertheless the intensity profile of theses fibers usually has a Gaussian shape. To be compatible with main amplifier section injection, the Gaussian intensity profile must then be transformed into `top-hat' profile. To reach the goal, we have recently developed an elegant and efficient solution based on a single-mode fiber which directly delivers a spatially coherent `top-hat' beam. In the conference, we will present this mJ-class top-hat all-fiber laser system, the results and the industrial prototype which can be used as a front-end of high-power lasers or as a seeder for other types of lasers.

  7. HAT-P-24b: An Inflated Hot Jupiter on a 3.36 Day Period Transiting a Hot, Metal-poor Star

    Science.gov (United States)

    Kipping, D. M.; Bakos, G. Á.; Hartman, J.; Torres, G.; Shporer, A.; Latham, D. W.; Kovács, Géza; Noyes, R. W.; Howard, A. W.; Fischer, D. A.; Johnson, J. A.; Marcy, G. W.; Béky, B.; Perumpilly, G.; Esquerdo, G. A.; Sasselov, D. D.; Stefanik, R. P.; Lázár, J.; Papp, I.; Sári, P.

    2010-12-01

    We report the discovery of HAT-P-24b, a transiting extrasolar planet orbiting the moderately bright V = 11.818 F8 dwarf star GSC 0774-01441, with a period P = 3.3552464 ± 0.0000071 days, transit epoch Tc = 2455216.97669 ± 0.00024 (BJD)11, and transit duration 3.653 ± 0.025 hr. The host star has a mass of 1.191 ± 0.042 M sun, radius of 1.317 ± 0.068 R sun, effective temperature 6373 ± 80 K, and a low metallicity of [Fe/H] = -0.16 ± 0.08. The planetary companion has a mass of 0.681 ± 0.031 M J and radius of 1.243 ± 0.072 R J yielding a mean density of 0.439 ± 0.069 g cm-3. By repeating our global fits with different parameter sets, we have performed a critical investigation of the fitting techniques used for previous Hungarian-made Automated Telescope planetary discoveries. We find that the system properties are robust against the choice of priors. The effects of fixed versus fitted limb darkening are also examined. HAT-P-24b probably maintains a small eccentricity of e = 0.052+0.022 -0.017, which is accepted over the circular orbit model with false alarm probability 5.8%. In the absence of eccentricity pumping, this result suggests that HAT-P-24b experiences less tidal dissipation than Jupiter. Due to relatively rapid stellar rotation, we estimate that HAT-P-24b should exhibit one of the largest known Rossiter-McLaughlin effect amplitudes for an exoplanet (ΔV RM ~= 95 m s-1) and thus a precise measurement of the sky-projected spin-orbit alignment should be possible. Based in part on observations obtained at the W. M. Keck Observatory, which is operated by the University of California and the California Institute of Technology. Keck time has been granted by NOAO and NASA.

  8. Teleskopische Generationsnarrative. Elisabeth Reicharts «Die Voest-Kinder» und Melitta Brezniks «Der Sommer hat lange auf sich warten lassen»

    OpenAIRE

    Elin Nesje Vestli

    2014-01-01

    This is a comparative reading of Die Voest-Kinder (2011) by E. Reichart and Der Sommer hat lange auf sich warten lassen (2013) by M. Breznik. Both novels participate in a recent trend in Austrian and German contemporary literature; they explore how history, primarily family history, is passed on in a trans-generational perspective: as transmitted silence. It is here that we find the origin of traumas in life and in these two novels, not only for those directly affected by the traumatic events...

  9. Effects of age on genetic influence on bone loss over 17 years in women: the Healthy Ageing Twin Study (HATS).

    Science.gov (United States)

    Moayyeri, Alireza; Hammond, Christopher J; Hart, Deborah J; Spector, Timothy D

    2012-10-01

    The rate of bone loss varies across the aging period via multiple complex mechanisms. Therefore, the role of genetic factors on bone loss may also change similarly. In this study, we investigated the effect of age on the genetic component of bone loss in a large twin-based longitudinal study. During 17 years of follow-up in TwinsUK and Healthy Ageing Twin Study (HATS), 15,491 hip and lumbar spine dual-energy X-ray absorptiometry (DXA) scans were performed in 7056 twins. Out of these subjects, 2716 female twins aged >35 years with at least two scans separated for >4 years (mean follow-up 9.7 years) were included in this analysis. We used a mixed-effects random-coefficients regression model to predict hip and spine bone mineral density (BMD) values for exact ages of 40, 45, 50, 55, 60, 65, 70, 75, and 80 years, with adjustment for baseline age, weight, height, and duration of hormone replacement therapy. We then estimated heritability of the changes in BMD measures between these age ranges. Heritability estimates for cross-sectional hip and spine BMD were high (ranging between 69% and 88%) at different ages. Heritability of change of BMD was lower and more variable, generally ranging from 0% to 40% for hip and 0% to 70% for spine; between age 40 and 45 years genetic factors explained 39.9% (95% confidence interval [CI], 25%-53%) of variance of BMD loss for total hip, 46.4% (95% CI, 32%-58%) for femoral neck, and 69.5% (95% CI, 59%-77%) for lumbar spine. These estimates decreased with increasing age, and there appeared to be no heritability of BMD changes after the age of 65 years. There was some evidence at the spine for shared genetic effects between cross-sectional and longitudinal BMD. Whereas genetic factors appear to have an important role in bone loss in early postmenopausal women, nongenetic mechanisms become more important determinants of bone loss with advanced age. Copyright © 2012 American Society for Bone and Mineral Research.

  10. The Census Taker's Hat

    Science.gov (United States)

    Portugués, Rubén

    Magnetic monopoles have rivaled black holes as the most beautiful and elusive entities in the world of theoretical physics since they were first considered in the early twentieth century. They naturally arise in electromagnetism and have immediate implications on the underlying symmetries, both dynamical and internal, at both the classical and quantum level. In this article we will recap some facts about magnetic monopoles in electromagnetism, in particular notions regarding their contribution to the angular momentum of a system and how these concepts are related to the quantization condition for the product of the charges of a fundamental electric pole and magnetic pole. We then proceed to consider magnetic poles in general relativity and briefly address similar considerations in this theory.

  11. Household wireless electroencephalogram hat

    Science.gov (United States)

    Szu, Harold; Hsu, Charles; Moon, Gyu; Yamakawa, Takeshi; Tran, Binh

    2012-06-01

    We applied Compressive Sensing to design an affordable, convenient Brain Machine Interface (BMI) measuring the high spatial density, and real-time process of Electroencephalogram (EEG) brainwaves by a Smartphone. It is useful for therapeutic and mental health monitoring, learning disability biofeedback, handicap interfaces, and war gaming. Its spec is adequate for a biomedical laboratory, without the cables hanging over the head and tethered to a fixed computer terminal. Our improved the intrinsic signal to noise ratio (SNR) by using the non-uniform placement of the measuring electrodes to create the proximity of measurement to the source effect. We computing a spatiotemporal average the larger magnitude of EEG data centers in 0.3 second taking on tethered laboratory data, using fuzzy logic, and computing the inside brainwave sources, by Independent Component Analysis (ICA). Consequently, we can overlay them together by non-uniform electrode distribution enhancing the signal noise ratio and therefore the degree of sparseness by threshold. We overcame the conflicting requirements between a high spatial electrode density and precise temporal resolution (beyond Event Related Potential (ERP) P300 brainwave at 0.3 sec), and Smartphone wireless bottleneck of spatiotemporal throughput rate. Our main contribution in this paper is the quality and the speed of iterative compressed image recovery algorithm based on a Block Sparse Code (Baranuick et al, IEEE/IT 2008). As a result, we achieved real-time wireless dynamic measurement of EEG brainwaves, matching well with traditionally tethered high density EEG.

  12. An unusual case of occupational asthma in a part time magician. He has got an allergy surprise from his top hat!

    Science.gov (United States)

    Liccardi, Gennaro; Billeri, L; Foglia, M; Sapio, C; De Giglio, M A R; D'Amato, G

    2014-09-01

    In this report we describe a case of respiratory allergy induced by an unusual occupational exposure to rabbit. The patient worked as a part-time magician in theatres and private parties and the most popular performance of his show was to pull out a white rabbit from a top hat. Unfortunately, a few minutes after the extraction of rabbit from top hat, the patient experienced the onset of upper and lower airway symptoms, and in some occasions he was forced to stop the show and to use short acting β₂agonists and intramuscular steroids. The results of SPT and evaluation of serological specific IgE (ImmunoCAP and ImmunoCAP ISAC IgE) revealed allergic sensitization to rabbit (Oryctolagus cuniculus) dander as well as to Parietaria and dust mites. ImmunoCAP ISAC IgE excluded allergic sensitization to other cross-reacting animal allergens. Rabbit constitutes a reliable risk factor for allergic sensitization in individuals working as professional / part-time magicians or as animators in some recreational settings (resorts, parties, charity shows, etc).

  13. Améliorer le fonctionnement sans disque de la distribution Red Hat grâce à la fusion de système de fichiers

    CERN Document Server

    Schweitzer, Pierre; Brarda, Loïc

    2011-01-01

    Le travail durant ce stage a permis de revoir, toute la gestion de la ferme de calcul sans disques qu'utilise l'expérience LHCb du CERN. Celle-ci est maintenant administrable par l'intermédiaire du logiciel de gestion Quattor et autorise à la fois l'administration du High Level Trigger (HLT) mais également du trigger L0 (contrairement à ce qui était initialement prévu). Cette nouvelle gestion, fortement indépendante des versions du système Linux utilisé, Scientific Linux CERN (SLC), permet le passage des CCPCs de SLC4 à SLC5 très facilement. Un résultat important est l'absence d'utilisation des outils Red Hat pour administer cette ferme, ce qui ouvre la voie à une future migration de l'ensemble de la ferme à SLC6, quand celle-ci sera prête. L'abandon des outils Red Hat a nécessité l'utilisation de la fusion de systèmes de fichiers pour assurer l'unicité d'un répertoire racine partagé par plusieurs machines. Jusqu'alors la fusion de systèmes de fichiers n'était pas utilisée, et seuls ...

  14. HAT-P-49b: a 1.7 M {sub J} planet transiting a bright 1.5 M {sub ☉} F-star

    Energy Technology Data Exchange (ETDEWEB)

    Bieryla, A.; Latham, D. W.; Buchhave, L. A.; Béky, B.; Falco, E.; Torres, G.; Noyes, R. W.; Berlind, P.; Calkins, M. C.; Esquerdo, G. A. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Hartman, J. D.; Bakos, G. Á.; Bhatti, W.; Csubry, Z.; Penev, K.; De Val-Borro, M. [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08544 (United States); Kovács, G. [Konkoly Observatory, Budapest 1121 (Hungary); Boisse, I. [Centro de Astrofísica, Universidade do Porto, Rua das Estrelas, 4150-762 Porto (Portugal); Lázár, J.; Papp, I., E-mail: abieryla@cfa.harvard.edu, E-mail: gbakos@astro.princeton.edu [Hungarian Astronomical Association (HAA), Budapest 1461 (Hungary); and others

    2014-04-01

    We report the discovery of the transiting extrasolar planet HAT-P-49b. The planet transits the bright (V = 10.3) slightly evolved F-star HD 340099 with a mass of 1.54 M {sub ☉} and a radius of 1.83 R {sub ☉}. HAT-P-49b is orbiting one of the 25 brightest stars to host a transiting planet which makes this a favorable candidate for detailed follow-up. This system is an especially strong target for Rossiter-McLaughlin follow-up due to the host star's fast rotation, 16 km s{sup –1}. The planetary companion has a period of 2.6915 days, mass of 1.73 M {sub J}, and radius of 1.41 R {sub J}. The planetary characteristics are consistent with that of a classical hot Jupiter but we note that this is the fourth most massive star to host a transiting planet with both M{sub p} and R{sub p} well determined.

  15. WASP-35b, WASP-48b and WASP-51b: Two new planets and an independent discovery of HAT-P-30b

    CERN Document Server

    Enoch, B; Barros, S C C; Brown, D J A; Cameron, A Collier; Faedi, F; Gillon, M; Hébrard, G; Lister, T A; Queloz, D; Santerne, A; Smalley, B; Street, R A; Triaud, A H M J; West, R G; Bouchy, F; Bento, J; Butters, O; Fossati, L; Haswell, C A; Hellier, C; Holmes, S; Jehen, E; Lendl, M; Maxted, P F L; McCormac, J; Miller, G R M; Moulds, V; Moutou, C; Norton, A J; Parley, N; Pepe, F; Pollacco, D; Segransan, D; Simpson, E; Skillen, I; Smith, A M S; Udry, S; Wheatley, P J

    2011-01-01

    We report the detection of WASP-35b, a planet transiting a metal-poor ([Fe/H] = -0.15) star in the Southern hemisphere, WASP-48b, an inflated planet which may have spun-up its slightly evolved host star of 1.75 R_sun in the Northern hemisphere, and the independent discovery of HAT-P-30b / WASP-51b, a new planet in the Northern hemisphere. Using WASP, RISE, FTS and TRAPPIST photometry, with CORALIE, SOPHIE and NOT spectroscopy, we determine that WASP-35b has a mass of 0.72 +/- 0.06 M_J and radius of 1.32 +/- 0.03 R_J, and orbits with a period of 3.16 days, WASP-48b has a mass of 0.98 +/- 0.09 M_J, radius of 1.67 +/- 0.08 R_J and orbits in 2.14 days, while WASP-51b, with an orbital period of 2.81 days, is found to have a mass of 0.76 +/- 0.05 M_J and radius of 1.42 +/- 0.04 R_J, agreeing with values of 0.71 +/- 0.03 M_J and 1.34 +/- 0.07 R_J reported for HAT-P-30b.

  16. HAT-P-49b: A 1.7 $M_J$ Planet Transiting a Bright 1.5 $M_\\odot$ F-Star

    CERN Document Server

    Bieryla, A; Bakos, G A; Bhatti, W; Kovacs, G; Boisse, I; Latham, D W; Buchhave, L A; Csubry, Z; Penev, K; de Val-Borro, M; Beky, B; Falco, E; Torres, G; Noyes, R W; Berlind, P; Calkins, M C; Esquerdo, G A; Lazar, J; Papp, I; Sari, P

    2014-01-01

    We report the discovery of the transiting extrasolar planet HAT-P-49b. The planet transits the bright (V = 10.3) slightly evolved F-star HD 340099 with a mass of 1.54 M_S and a radius of 1.83 R_S. HAT-P-49b is orbiting one of the 25 brightest stars to host a transiting planet which makes this a favorable candidate for detailed follow-up. This system is an especially strong target for Rossiter-McLaughlin follow-up due to the fast rotation of the host star, 16 km/s. The planetary companion has a period of 2.6915 d, mass of 1.73 M_J and radius of 1.41 R_J. The planetary characteristics are consistent with that of a classical hot Jupiter but we note that this is the fourth most massive star to host a transiting planet with both M_p and R_p well determined.

  17. KEPLER'S OPTICAL SECONDARY ECLIPSE OF HAT-P-7b AND PROBABLE DETECTION OF PLANET-INDUCED STELLAR GRAVITY DARKENING

    Energy Technology Data Exchange (ETDEWEB)

    Morris, Brett M.; Deming, Drake [Department of Astronomy, University of Maryland, College Park, MD 20742 (United States); Mandell, Avi M. [Goddard Center for Astrobiology, NASA' s Goddard Space Flight Center, Greenbelt, MD 20771 (United States)

    2013-02-20

    We present observations spanning 355 orbital phases of HAT-P-7 observed by Kepler from 2009 May to 2011 March (Q1-9). We find a shallower secondary eclipse depth than initially announced, consistent with a low optical albedo and detection of nearly exclusively thermal emission, without a reflected light component. We find an approximately 10 ppm perturbation to the average transit light curve near phase -0.02 that we attribute to a temperature decrease on the surface of the star, phased to the orbit of the planet. This cooler spot is consistent with planet-induced gravity darkening, slightly lagging the sub-planet position due to the finite response time of the stellar atmosphere. The brightness temperature of HAT-P-7b in the Kepler bandpass is T{sub B} = 2733 {+-} 21 K and the amplitude of the deviation in stellar surface temperature due to gravity darkening is approximately -0.18 K. The detection of the spot is not statistically unequivocal due its small amplitude, though additional Kepler observations should be able to verify the astrophysical nature of the anomaly.

  18. The Effectiveness of Training Program Based on the Six Hats Model in Developing Creative Thinking Skills and Academic Achievements in the Arabic Language Course for Gifted and Talented Jordanian Students

    Science.gov (United States)

    Ziadat, Ayed H.; Al Ziyadat, Mohammad T.

    2016-01-01

    The main purpose of this study was to determine the effectiveness of a training program based on the six hats model in developing creative thinking skills and academic achievements in the Arabic language for gifted and talented Jordanian students. The study sample consisted of 59 gifted male and female students of the 7th grade from King Abdullah…

  19. Teleskopische Generationsnarrative. Elisabeth Reicharts «Die Voest-Kinder» und Melitta Brezniks «Der Sommer hat lange auf sich warten lassen»

    Directory of Open Access Journals (Sweden)

    Elin Nesje Vestli

    2014-05-01

    Full Text Available This is a comparative reading of Die Voest-Kinder (2011 by E. Reichart and Der Sommer hat lange auf sich warten lassen (2013 by M. Breznik. Both novels participate in a recent trend in Austrian and German contemporary literature; they explore how history, primarily family history, is passed on in a trans-generational perspective: as transmitted silence. It is here that we find the origin of traumas in life and in these two novels, not only for those directly affected by the traumatic events, but also for the following generations. Traumatic experi­ences are transmitted by what S. Weigel, based on the psychoanalyst H. Faimberg, descri­bes as «telescoping».

  20. "Das Konkrete ist das Abstrakte, an das man sich schließlich gewöhnt hat." (Laurent Schwartz) Über den Ablauf des mathematischen Verstehens

    Science.gov (United States)

    Lowsky, Martin

    Die im Titel genannte Aussage findet sich in den Lebenserinnerungen von Laurent Schwartz (1915-2002), einem der fruchtbarsten Mathematiker, Mitglied der Gruppe Bourbaki. Im Original lautet die Aussage: "un objet concret est un objet abstrait auquel on a fini par s'habituer." Schwartz erläutert sie am Beispiel des Integrals über {e^{-1/2{x^2}}} , das den Wert Wurzel aus 2π hat und in dem sich also die Zahlen e und π verknüpfen. Was Schwartz aber vor allem ausdrücken will, ist dies: Das mathematische Verständnisd geht langsam vor sich und es bedarf der Anstrengung. "Es ist eine Frage der Zeit und der Energie", sagt Schwartz, und gerade dies mache es so schwer, die höhere Mathematik unter das Volk zu bringen. Das Lernen und Lehren von Mathematik laufe eben mühevoll und langsam ab.

  1. H2: entanglement, probability density function, confined Kratzer oscillator, universal potential and (Mexican hat- or bell-type) potential energy curves

    CERN Document Server

    Van Hooydonk, G

    2011-01-01

    We review harmonic oscillator theory for closed, stable quantum systems. The H2 potential energy curve (PEC) of Mexican hat-type, calculated with a confined Kratzer oscillator, is better than the Rydberg-Klein-Rees (RKR) H2 PEC. Compared with QM, the theory of chemical bonding is simplified, since a confined Kratzer oscillator gives the long sought for universal function, once called the Holy Grail of Molecular Spectroscopy. This is validated with HF, I2, N2 and O2 PECs. We quantify the entanglement of spatially separated H2 quantum states, which gives a braid view. The equal probability for H2, originating either from HA+HB or HB+HA, is quantified with a Gauss probability density function. At the Bohr scale, confined harmonic oscillators behave properly at all extremes of bound two-nucleon quantum systems and are likely to be useful also at the nuclear scale.

  2. Characterization of yeast histone H3-specific type B histone acetyltransferases identifies an ADA2-independent Gcn5p activity

    Directory of Open Access Journals (Sweden)

    Parthun Mark R

    2004-07-01

    Full Text Available Abstract Background The acetylation of the core histone NH2-terminal tails is catalyzed by histone acetyltransferases. Histone acetyltransferases can be classified into two distinct groups (type A and B on the basis of cellular localization and substrate specificity. Type B histone acetyltransferases, originally defined as cytoplasmic enzymes that acetylate free histones, have been proposed to play a role in the assembly of chromatin through the acetylation of newly synthesized histones H3 and H4. To date, the only type B histone acetyltransferase activities identified are specific for histone H4. Results To better understand the role of histone acetylation in the assembly of chromatin structure, we have identified additional type B histone acetyltransferase activities specific for histone H3. One such activity, termed HatB3.1, acetylated histone H3 with a strong preference for free histones relative to chromatin substrates. Deletion of the GCN5 and ADA3 genes resulted in the loss of HatB3.1 activity while deletion of ADA2 had no effect. In addition, Gcn5p and Ada3p co-fractionated with partially purified HatB3.1 activity while Ada2p did not. Conclusions Yeast extracts contain several histone acetyltransferase activities that show a strong preference for free histone H3. One such activity, termed HatB3.1, appears to be a novel Gcn5p-containing complex which does not depend on the presence of Ada2p.

  3. Avaliação da qualidade de vida de mulheres com HIV/AIDS através do HAT-QoL

    Directory of Open Access Journals (Sweden)

    Galvão Marli Teresinha Gimeniz

    2004-01-01

    Full Text Available Com objetivo de avaliar qualidade de vida entre mulheres portadoras de HIV/AIDS, aplicou-se o HIV/AIDS ­ Quality of Life test (HAT-QoL, instrumento específico para indivíduos infectados pelo HIV. Compõe-se de 42 questões e divide-se em nove domínios com questões sobre diversos aspectos de vida dos portadores. A escala foi submetida à tradução reversa e pré-teste. Sua forma definitiva foi aplicada entre 73 mulheres com HIV/AIDS. Os resultados, após análise estatística, indicaram que os domínios mais comprometidos foram: "Preocupações financeiras", "Preocupação com sigilo sobre a infecção", "Atividades sexuais" e "Preocupações com a saúde". Esses resultados foram atribuídos ao fato das mulheres apresentarem, além da infecção, uma situação sócio-econômica precária que, provavelmente, também interferiu negativamente em sua qualidade de vida. Concluímos que a escala HAT-QoL foi adequada para a população de mulheres portadoras de HIV deste estudo, apesar de ser originária de uma cultura diferente da brasileira, já que sua aplicação produziu resultados semelhantes aos da literatura internacional.

  4. The Parameterization of Top-Hat Particle Sensors with Microchannel-Plate-Based Detection Systems and its Application to the Fast Plasma Investigation on NASA's Magnetospheric MultiScale Mission

    Science.gov (United States)

    Gershman, Daniel J.; Gliese, Ulrik; Dorelli, John C.; Avanov, Levon A.; Barrie, Alexander C.; Chornay, Dennis J.; MacDonald, Elizabeth A.; Holland, Matthew P.; Pollock, Craig J.

    2015-01-01

    The most common instrument for low energy plasmas consists of a top-hat electrostatic analyzer geometry coupled with a microchannel-plate (MCP)-based detection system. While the electrostatic optics for such sensors are readily simulated and parameterized during the laboratory calibration process, the detection system is often less well characterized. Furthermore, due to finite resources, for large sensor suites such as the Fast Plasma Investigation (FPI) on NASA's Magnetospheric Multiscale (MMS) mission, calibration data are increasingly sparse. Measurements must be interpolated and extrapolated to understand instrument behavior for untestable operating modes and yet sensor inter-calibration is critical to mission success. To characterize instruments from a minimal set of parameters we have developed the first comprehensive mathematical description of both sensor electrostatic optics and particle detection systems. We include effects of MCP efficiency, gain, scattering, capacitive crosstalk, and charge cloud spreading at the detector output. Our parameterization enables the interpolation and extrapolation of instrument response to all relevant particle energies, detector high voltage settings, and polar angles from a small set of calibration data. We apply this model to the 32 sensor heads in the Dual Electron Sensor (DES) and 32 sensor heads in the Dual Ion Sensor (DIS) instruments on the 4 MMS observatories and use least squares fitting of calibration data to extract all key instrument parameters. Parameters that will evolve in flight, namely MCP gain, will be determined daily through application of this model to specifically tailored in-flight calibration activities, providing a robust characterization of sensor suite performance throughout mission lifetime. Beyond FPI, our model provides a valuable framework for the simulation and evaluation of future detection system designs and can be used to maximize instrument understanding with minimal calibration

  5. Tectonic geomorphology and volcano-tectonic interaction in the eastern boundary of the Southern Cascades (Hat Creek Graben region, California, USA

    Directory of Open Access Journals (Sweden)

    Engielle Mae Raot-raot Paguican

    2016-07-01

    Full Text Available The eastern boundary of the Southern Cascades (Hat Creek Graben region, California, USA, is an extensively faulted volcanic corridor between the Cascade Range and Modoc Plateau. The east-west extending region is in the transition zone between the convergence and subduction of the Gorda Plate underneath the North American Plate; north-south shortening within the Klamath Mountain region; and transcurrent movement in the Walker Lane. We describe the geomorphological and tectonic features, their alignment and distribution, in order to understand the tectonic geomorphology and volcano-tectonic relationships. One outcome of the work is a more refined morpho-structural description that will affect future hazard assessment in the area.A database of volcanic centers and structures was created from interpretations of topographic models generated from satellite images. Volcanic centers in the region were classified by morphological type into cones, sub-cones, shields and massifs. A second classification by height separated the bigger and smaller edifices and revealed an evolutionary trend. Poisson Nearest Neighbor analysis shows that bigger volcanoes are spatially dispersed while smaller ones are clustered. Using volcano centroid locations, about 90 lineaments consisting of at least three centers within 6km of one another were found, revealing that preferential north-northwest directed pathways control the transport of magma from the source to the surface, consistent with the strikes of the major fault systems. Most of the volcano crater openings are perpendicular to the maximum horizontal stress, expected for extensional environments with dominant normal regional faults. These results imply that the extension of the Hat Creek Graben region and impingement of the Walker Lane is accommodated mostly by extensional faults and partly by the intrusions that formed the volcanoes. Early in the history of a volcano or volcano cluster, melt produced at depth in the

  6. HATS (High Altitude Thermal Sounder): a passive sensor solution to 3D high-resolution mapping of upper atmosphere dynamics (Conference Presentation)

    Science.gov (United States)

    Gordley, Larry; Marshall, Benjamin T.; Lachance, Richard L.

    2016-10-01

    This presentation introduces a High Altitude Thermal Sensor (HATS) that has the potential to resolve the thermal structure of the upper atmosphere (cloud top to 100km) with both horizontal and vertical resolution of 5-7 km or better. This would allow the complete characterization of the wave structures that carry weather signature from the underlying atmosphere. Using a novel gas correlation technique, an extremely high-resolution spectral scan is accomplished by measuring a Doppler modulated signal as the atmospheric thermal scene passes through the HATS 2D FOV. This high spectral resolution, difficult to impossible to achieve with any other passive technique, enables the separation of radiation emanating at high altitudes from that emanating at low altitudes. A principal component analysis of these modulation signals then exposes the complete thermal structure of the upper atmosphere. We show that nadir sounding from low earth orbit, using various branches of CO2 emission in the 17 to 15 micron region, with sufficient spectral resolution and spectral measurement range, can distinguish thermal energy that peaks at various altitudes. By observing the up-welling atmospheric emission through a low pressure (Doppler broadened) gas cell, as the scene passes through our FOV, a modulation signal is created as the atmospheric emission lines are shifted through the spectral position of the gas cell absorption lines. The modulation signal is shown to be highly correlated to the emission coming from the spectral location of the gas cell lines relative to the atmospheric emission lines. This effectively produces a scan of the atmospheric emission with a Doppler line resolution. Similar to thermal sounding of the troposphere, a principal component analysis of the modulation signal can be used to produce an altitude resolved profile, given a reasonable a priori temperature profile. It is then shown that with the addition of a limb observation with one CO2 broadband channel

  7. Tectonic geomorphology and volcano-tectonic interaction in the eastern boundary of the Southern Cascades (Hat Creek Graben region), California, USA

    Science.gov (United States)

    Paguican, Engielle Mae; Bursik, Marcus

    2016-07-01

    The eastern boundary of the Southern Cascades (Hat Creek Graben region), California, USA, is an extensively faulted volcanic corridor between the Cascade Range and Modoc Plateau. The east-west extending region is in the transition zone between the convergence and subduction of the Gorda Plate underneath the North American Plate; north-south shortening within the Klamath Mountain region; and transcurrent movement in the Walker Lane. We describe the geomorphological and tectonic features, their alignment and distribution, in order to understand the tectonic geomorphology and volcano-tectonic relationships. One outcome of the work is a more refined morpho-structural description that will affect future hazard assessment in the area. A database of volcanic centers and structures was created from interpretations of topographic models generated from satellite images. Volcanic centers in the region were classified by morphological type into cones, sub-cones, shields and massifs. A second classification by height separated the bigger and smaller edifices and revealed an evolutionary trend. Poisson Nearest Neighbor analysis shows that bigger volcanoes are spatially dispersed while smaller ones are clustered. Using volcano centroid locations, about 90 lineaments consisting of at least three centers within 6km of one another were found, revealing that preferential north-northwest directed pathways control the transport of magma from the source to the surface, consistent with the strikes of the major fault systems. Most of the volcano crater openings are perpendicular to the maximum horizontal stress, expected for extensional environments with dominant normal regional faults. These results imply that the extension of the Hat Creek Graben region and impingement of the Walker Lane is accommodated mostly by extensional faults and partly by the intrusions that formed the volcanoes. Early in the history of a volcano or volcano cluster, melt produced at depth in the region propagates

  8. In vitro antioxidant capacity of tea of Echinodorus grandiforus, “leather hat,” in Wistar rat liver

    Directory of Open Access Journals (Sweden)

    RAFAELA F. LUNARDI

    2014-09-01

    Full Text Available Oxidative stress has been considered as one of the factors responsible for hepatic diseases, which sometimes require new ways of treatment. The present study aimed to evaluate the in vitro antioxidant capacity of the tea of Echinodorus grandiforus (“leather hat” plant in rat liver. Different preparations of tea were evaluated for phenolic composition, antioxidant activity by DPPH assay and ability to inhibit lipid peroxidation induced by copper sulfate. The antioxidant activity was assessed in liver tissue treated with sodium azide in the presence or absence of tea by assays for lipid peroxidation (TBARS, protein oxidation (carbonyl and the antioxidant enzymes catalase (CAT and superoxide dismutase (SOD. The results show that different preparations of tea are important sources of polyphenols and contain theobromine, catechin and vitexin. Furthermore, the results indicate that this tea exhibits an antioxidant activity by its ability to scavenge DPPH radical. Different preparations of tea prevented damage to lipids and proteins induced by sodium azide, as well as assisting in restoring CAT and SOD activities. Thus, it can be seen that E. grandiforus tea had antioxidant activity in serum and liver being able to prevent oxidative damages generated by sodium azide.

  9. Time-resolved luminescence biosensor for continuous activity detection of protein acetylation-related enzymes based on DNA-sensitized terbium(III) probes.

    Science.gov (United States)

    Han, Yitao; Li, Hao; Hu, Yufang; Li, Pei; Wang, Huixia; Nie, Zhou; Yao, Shouzhuo

    2015-09-15

    Protein acetylation of histone is an essential post-translational modification (PTM) mechanism in epigenetic gene regulation, and its status is reversibly controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Herein, we have developed a sensitive and label-free time-resolved luminescence (TRL) biosensor for continuous detection of enzymatic activity of HATs and HDACs, respectively, based on acetylation-mediated peptide/DNA interaction and Tb(3+)/DNA luminescent probes. Using guanine (G)-rich DNA-sensitized Tb(3+) luminescence as the output signal, the polycationic substrate peptides interact with DNA with high affinity and subsequently replace Tb(3+), eliminating the luminescent signal. HAT-catalyzed acetylation remarkably reduces the positive charge of the peptides and diminishes the peptide/DNA interaction, resulting in the signal on detection via recovery of DNA-sensitized Tb(3+) luminescence. With this TRL sensor, HAT (p300) can be sensitively detected with a wide linear range from 0.2 to 100 nM and a low detection limit of 0.05 nM. The proposed sensor was further used to continuously monitor the HAT activity in real time. Additionally, the TRL biosensor was successfully applied to evaluating HAT inhibition by two specific inhibitors, anacardic acid and C464, and satisfactory Z'-factors above 0.73 were obtained. Moreover, this sensor is feasible to continuously monitor the HDAC (Sirt1)-catalyzed deacetylation with a linear range from 0.5 to 500 nM and a detection limit of 0.5 nM. The proposed sensor is a convenient, sensitive, and mix-and-read assay, presenting a promising platform for protein acetylation-targeted epigenetic research and drug discovery.

  10. Application Study on Six Thinking Hats Thinking Mode in Clinical Teaching of Traditional Chinese Medicine%“六顶思考帽”思维方式在中医临床带教中应用探讨

    Institute of Scientific and Technical Information of China (English)

    丁永利

    2016-01-01

    “六顶思考帽”的提出者是马耳他医学博士爱德华•德•波诺,其核心思想是建立一种积极热情、勇敢无畏且富有创造精神的科学思维模式。该模式采用白、红、绿、黑、黄、蓝六种颜色不同的帽子代表六种不同的思维模式。该文主要介绍了“六顶思考帽”的基本定义,阐述了“六顶思考帽”在中医临床带教中的应用,强调了多维思考的重要性,通过该文研究,最终实现中医临床研究的思考模式。%The presenter of six thinking hats is Malta medical doctor Edward de Bono, the core concept is to build a scien-tific thinking mode that is positive and enthusiastic, brave and full of creative spirit. The model adopts hats of six colors such as white, red, green, blank, yellow and blue to represent six thinking models. The paper mainly introduces the basic definition of six thinking hats, elaborates the application of six thinking hats in clinical teaching of traditional Chinese medicine and emphasizes the importance of multidimensional reflections, and the thinking model of the clinical research of traditional Chinese medicine will be finally realized by the research of this paper.

  11. Demonstrating High-precision, Multi-band Transit Photometry with MuSCAT: A Case for HAT-P-14b

    CERN Document Server

    Fukui, Akihiko; Kawashima, Yui; Kusakabe, Nobuhiko; Onitsuka, Masahiro; Ryu, Tsuguru; Ikoma, Masahiro; Yanagisawa, Kenshi; Izumiura, Hideyuki

    2015-01-01

    The Multicolor Simultaneous Camera for studying Atmospheres of Transiting exoplanets (MuSCAT) is an optical three-band (g'_2-, r'_2-, and z_{s,2}-band) imager, recently developed for the 188cm telescope at Okayama Astrophysical Observatory with the aim of validating and characterizing transiting planets. In a pilot observation with MuSCAT, we observed a primary transit of HAT-P-14b, a high-surface gravity (g_p=38 ms^{-2}) hot Jupiter around a bright (V=10) F-type star. From a 2.9-hour observation, we achieve the 5-min binned photometric precisions of 0.028%, 0.022%, and 0.024% in the g'_2, r'_2, and z_{s,2} bands, respectively, providing the highest-quality photometric data for this planet. Combining these results with those of previous observations, we search for variations of transit timing and duration over five years, as well as variations of planet-star radius ratio (R_p/R_s) with wavelength, but find no considerable variation in any parameters. On the other hand, using the transit-subtracted light curve...

  12. The EVIL-MC Model for Ellipsoidal Variations of Planet-Hosting Stars and Applications to the HAT-P-7 System

    CERN Document Server

    Jackson, Brian K; Barnes, Jason W; Deming, L Drake; Showman, Adam P; Fortney, Jonathan J

    2012-01-01

    We present a new model for Ellipsoidal Variations Induced by a Low-Mass Companion, the EVIL-MC model. We employ several approximations appropriate for planetary systems to substantially increase the computational efficiency of our model relative to more general ellipsoidal variation models and improve upon the accuracy of simpler models. This new approach gives us a unique ability to rapidly and accurately determine planetary system parameters. We use the EVIL-MC model to analyze Kepler Quarter 0-2 (Q0-2) observations of the HAT-P-7 system, an F-type star orbited by a nearly Jupiter-mass companion. Our analysis corroborates previous estimates of the planet-star mass ratio q = (1.10 +/- 0.06) x 10^(-3), and we have revised the planet's dayside brightness temperature to 2680 +10/-20 K. We also find a large difference between the day- and nightside planetary flux, with little nightside emission. Preliminary dynamical+radiative modeling of the atmosphere indicates this result is qualitatively consistent with high...

  13. Spitzer Secondary Eclipses of the Dense, Modestly-irradiated, Giant Exoplanet HAT-P-20b Using Pixel-Level Decorrelation

    CERN Document Server

    Deming, Drake; Kammer, Joshua; Fulton, Benjamin J; Ingalls, James; Carey, Sean; Burrows, Adam; Fortney, Jonathan J; Todorov, Kamen; Agol, Eric; Cowan, Nicolas; Desert, Jean-Michel; Fraine, Jonathan; Langton, Jonathan; Morley, Caroline; Showman, Adam P

    2014-01-01

    HAT-P-20b is a giant exoplanet that orbits a metal-rich star. The planet itself has a high total density, suggesting that it may also have a high metallicity in its atmosphere. We analyze two eclipses of the planet in each of the 3.6- and 4.5 micron bands of Warm Spitzer. These data exhibit intra-pixel detector sensitivity fluctuations that were resistant to traditional decorrelation methods. We have developed a simple, powerful, and radically different method to correct the intra-pixel effect for Warm Spitzer data, which we call pixel-level decorrelation (PLD). PLD corrects the intra-pixel effect very effectively, but without explicitly using - or even measuring - the fluctuations in the apparent position of the stellar image. We illustrate and validate PLD using synthetic and real data, and comparing the results to previous analyses. PLD can significantly reduce or eliminate red noise in Spitzer secondary eclipse photometry, even for eclipses that have proven to be intractable using other methods. Our succe...

  14. Broad-band spectrophotometry of the hot Jupiter HAT-P-12b from the near-UV to the near-IR

    CERN Document Server

    Mallonn, M; Weingrill, J; von Essen, C; Strassmeier, K G; Piotto, G; Pagano, I; Scandariato, G; Csizmadia, Sz; Herrero, E; Sada, P V; Dhillon, V S; Marsh, T R; Künstler, A; Bernt, I; Granzer, T

    2015-01-01

    The detection of trends or gradients in the transmission spectrum of extrasolar planets is possible with observations at very low spectral resolution. Transit measurements of sufficient accuracy using selected broad-band filters allow for an initial characterization of the atmosphere of the planet. We obtained time series photometry of 20 transit events and analyzed them homogeneously, along with eight light curves obtained from the literature. In total, the light curves span a range from 0.35 to 1.25 microns. During two observing seasons over four months each, we monitored the host star to constrain the potential influence of starspots on the derived transit parameters. We rule out the presence of a Rayleigh slope extending over the entire optical wavelength range, a flat spectrum is favored for HAT-P-12b with respect to a cloud-free atmosphere model spectrum. A potential cause of such gray absorption is the presence of a cloud layer at the probed latitudes. Furthermore, in this work we refine the transit pa...

  15. Uncertainties of the Gravity Recovery and Climate Experiment time-variable gravity-field solutions based on three-cornered hat method

    Science.gov (United States)

    Ferreira, Vagner G.; Montecino, Henry D. C.; Yakubu, Caleb I.; Heck, Bernhard

    2016-01-01

    Currently, various satellite processing centers produce extensive data, with different solutions of the same field being available. For instance, the Gravity Recovery and Climate Experiment (GRACE) has been monitoring terrestrial water storage (TWS) since April 2002, while the Center for Space Research (CSR), the Jet Propulsion Laboratory (JPL), the GeoForschungsZentrum (GFZ), and the Groupe de Recherche de Géodésie Spatiale (GRGS) provide individual monthly solutions in the form of Stokes coefficients. The inverted TWS maps (or the regionally averaged values) from these coefficients are being used in many applications; however, as no ground truth data exist, the uncertainties are unknown. Consequently, the purpose of this work is to assess the quality of each processing center by estimating their uncertainties using a generalized formulation of the three-cornered hat (TCH) method. Overall, the TCH results for the study period of August 2002 to June 2014 indicate that at a global scale, the CSR, GFZ, GRGS, and JPL presented uncertainties of 9.4, 13.7, 14.8, and 13.2 mm, respectively. At a basin scale, the overall good performance of the CSR was observed at 91 river basins. The TCH-based results were confirmed by a comparison with an ensemble solution from the four GRACE processing centers.

  16. The optical transmission spectrum of the hot Jupiter HAT-P-32b: clouds explain the absence of broad spectral features?

    CERN Document Server

    Gibson, N P; Barstow, J K; Evans, T M; Fletcher, L N; Irwin, P G J

    2013-01-01

    We report Gemini-North GMOS observations of the inflated hot Jupiter HAT-P-32b during two primary transits. We simultaneously observed two comparison stars and used differential spectro-photometry to produce multi-wavelength light curves. 'White' light curves and 29 'spectral' light curves were extracted for each transit and analysed to refine the system parameters and produce transmission spectra from 520-930nm in ~14nm bins. The light curves contain time-varying white noise as well as time-correlated noise, and we used a Gaussian process model to fit this complex noise model. Common mode corrections derived from the white light curve fits were applied to the spectral light curves which significantly improved our precision, reaching typical uncertainties in the transit depth of ~2x10^-4, corresponding to about half a pressure scale height. The low resolution transmission spectra are consistent with a featureless model, and we can confidently rule out broad features larger than about one scale height. The abs...

  17. A piacorientáció hatása a vállalati versenyképességre = The Impact of Market Orientation on Competitiveness

    OpenAIRE

    Gyulavári, Tamás; Csepeti, Ádám; Nagy, Gábor

    2012-01-01

    A tanulmány a piacorientáció és a versenyképesség összefüggését elemzi egy átfogóbb koncepcionális modell keretében, amely a piacorientáció szervezeti előzményeire, a piacorientáció egyes dimenziói és a vállalati teljesítmény közötti kapcsolatra, valamint a környezetből származó bizonytalanság moderáló hatásaira fókuszál. Ahhoz, hogy növeljük a tanulmány érvényességét, az elemzés során olyan változókat kontrolláltunk, amelyekről úgy véltük, hogy a piacorientáció mellett szintén befolyásolhatj...

  18. 3.6 and 4.5 $\\mu$m ${\\it Spitzer}$ Phase Curves of the Highly-Irradiated Hot Jupiters WASP-19b and HAT-P-7b

    CERN Document Server

    Wong, Ian; Kataria, Tiffany; Lewis, Nikole K; Burrows, Adam; Fortney, Jonathan J; Schwartz, Joel; Shporer, Avi; Agol, Eric; Cowan, Nicholas B; Deming, Drake; Desert, Jean-Michel; Fulton, Benjamin J; Howard, Andrew W; Langton, Jonathan; Laughlin, Gregory; Showman, Adam P; Todorov, Kamen

    2016-01-01

    We analyze full-orbit phase curve observations of the transiting hot Jupiters WASP-19b and HAT-P-7b at 3.6 and 4.5 $\\mu$m obtained using the Spitzer Space Telescope. For WASP-19b, we measure secondary eclipse depths of $0.485\\%\\pm 0.024\\%$ and $0.584\\%\\pm 0.029\\%$ at 3.6 and 4.5 $\\mu$m, which are consistent with a single blackbody with effective temperature $2372 \\pm 60$ K. The measured 3.6 and 4.5 $\\mu$m secondary eclipse depths for HAT-P-7b are $0.156\\%\\pm 0.009\\%$ and $0.190\\%\\pm 0.006\\%$, which are well-described by a single blackbody with effective temperature $2667\\pm 57$ K. Comparing the phase curves to the predictions of one-dimensional and three-dimensional atmospheric models, we find that WASP-19b's dayside emission is consistent with a model atmosphere with no dayside thermal inversion and moderately efficient day-night circulation. We also detect an eastward-shifted hotspot, suggesting the presence of a superrotating equatorial jet. In contrast, HAT-P-7b's dayside emission suggests a dayside therm...

  19. Egyetemi funkciók és helyi gazdasági hatások: módszertani problémák és lehetséges megoldások

    OpenAIRE

    Kotosz, Balázs; Lukovics, Miklós (ed.); Zuti, Bence; Molnár, Gabriella

    2016-01-01

    Napjainkban egy egyetem jelenléte számos dimenzió mentén hozzáadott értéket teremt abban a térségben, ahol működik, és egyre inkább előtérbe kerül az a felismerés, hogy helyi gazdaságfejlesztésre gyakorolt hatása számottevő. Ez a jelenség egyre nagyobb igényt támaszt olyan tanulmányok iránt, amelyek ezeknek az intézményeknek a gazdasági hatását számszerűsítik. Számos módszer létezik már ezeknek a hatásoknak a mérésére, amelyek kisebbnagyobb mértékben eltérnek egymástól. Ez a di...

  20. Transcriptional Regulation by KLF6, A Novel Tumor Suppressor Gene in Prostate Cancer, Through Interaction with HATS and HDACS

    Science.gov (United States)

    2006-03-01

    adipocyte proteins, including leptin and adipsin (1, 2). Our previous work (5) has explored the activity of a tran- scription factor, KLF6 (also known as...Diabetes and Digestive Kidney Diseases (RO1DK37340, RO1DK56621 [S.L.F]; 1 K24 DK 60498-01 [M.S.]; T32 DK-07792 [S.T.K.]), the US Department of Defense

  1. Phenolic Constituents of Medicinal Plants with Activity against Trypanosoma brucei

    Directory of Open Access Journals (Sweden)

    Ya Nan Sun

    2016-04-01

    Full Text Available Neglected tropical diseases (NTDs affect over one billion people all over the world. These diseases are classified as neglected because they impact populations in areas with poor financial conditions and hence do not attract sufficient research investment. Human African Trypanosomiasis (HAT or sleeping sickness, caused by the parasite Trypanosoma brucei, is one of the NTDs. The current therapeutic interventions for T. brucei infections often have toxic side effects or require hospitalization so that they are not available in the rural environments where HAT occurs. Furthermore, parasite resistance is increasing, so that there is an urgent need to identify novel lead compounds against this infection. Recognizing the wide structural diversity of natural products, we desired to explore and identify novel antitrypanosomal chemotypes from a collection of natural products obtained from plants. In this study, 440 pure compounds from various medicinal plants were tested against T. brucei by in a screening using whole cell in vitro assays. As the result, twenty-two phenolic compounds exhibited potent activity against cultures of T. brucei. Among them, eight compounds—4, 7, 11, 14, 15, 18, 20, and 21—showed inhibitory activity against T. brucei, with IC50 values below 5 µM, ranging from 0.52 to 4.70 μM. Based on these results, we attempt to establish some general trends with respect to structure-activity relationships, which indicate that further investigation and optimization of these derivatives might enable the preparation of potentially useful compounds for treating HAT.

  2. The optical transmission spectrum of the hot Jupiter HAT-P-32b: clouds explain the absence of broad spectral features?

    Science.gov (United States)

    Gibson, N. P.; Aigrain, S.; Barstow, J. K.; Evans, T. M.; Fletcher, L. N.; Irwin, P. G. J.

    2013-12-01

    We report Gemini-North Gemini Multi-Object Spectrograph observations of the inflated hot Jupiter HAT-P-32b during two primary transits. We simultaneously observed two comparison stars and used differential spectrophotometry to produce multiwavelength light curves. `White' light curves and 29 `spectral' light curves were extracted for each transit and analysed to refine the system parameters and produce transmission spectra from 520 to 930 nm in ≈14 nm bins. The light curves contain time-varying white noise as well as time-correlated noise, and we used a Gaussian process model to fit this complex noise model. Common mode corrections derived from the white light-curve fits were applied to the spectral light curves which significantly improved our precision, reaching typical uncertainties in the transit depth of ˜2 × 10-4, corresponding to about half a pressure scale height. The low-resolution transmission spectra are consistent with a featureless model, and we can confidently rule out broad features larger than about one scale height. The absence of Na/K wings or prominent TiO/VO features is most easily explained by grey absorption from clouds in the upper atmosphere, masking the spectral features. However, we cannot confidently rule out clear atmosphere models with low abundances (˜10-3 solar) of TiO, VO or even metal hydrides masking the Na and K wings. A smaller scale height or ionization could also contribute to muted spectral features, but alone are unable to account for the absence of features reported here.

  3. Opisthorchis viverrini and Haplorchis taichui: development of a multiplex PCR assay for their detection and differentiation using specific primers derived from HAT-RAPD.

    Science.gov (United States)

    Wongsawad, Chalobol; Wongsawad, Pheravut

    2012-10-01

    Specific primers for the detection of Opisthorchis viverrini and Haplorchis taichui were investigated by using the HAT-RAPD PCR method. Fourteen arbitrary primers (Operon Technologies) were performed for the generation of polymorphic DNA profiles. The results showed that a 319 bp fragment generated from the OPA-04 primer was expected to be O. viverrini-specific while a 256 bp fragment generated from the OPP-11 primer was considered to be H. taichui-specific. Based on each sequence data, two pairs of specific primers were designed and sequences of each primer were as follows; H. taichui; Hapt_F5'-GGCCAACGCAATCGTCATCC-3'and Hapt_R1 5'-CTCTCGACCTCCTCTAGAAT-3' which yielded a 170 bp PCR product. For O. viverrini, OpV-1F: 5'-AATCGGGCTGCATATTGACCGAT-3' and OpV-1R: 5'-CGGTGTTGCTTATTTTGCAGACAA-3' which generated a 319 bp PCR product. These specific primers were tested for efficacy and specific detection for all parasites DNA samples. The results showed that 170 and 319 bp specific PCR products were generated as equivalent to positive result in H. taichui and O. viverrini, respectively by having no cross-reaction with any parasites tested. PCR conditions are recommended at 68°C annealing temperature and with 0.5 mM magnesium chloride (Mg Cl(2)). Additionally, specific primers developed in this study were effective to determine the presence of both parasites in fish and snail intermediate hosts, which the DNA of O. viverrini was artificially spiked since it is rarely found in northern Thailand. The H. taichui and O. viverrini-specific primers successfully developed in this study can be use for epidemiological monitoring, preventing management and control programs.

  4. 六帽思维是深度沟通的桥梁%Six-hat Thinking is the Bridge of Depth Communication

    Institute of Scientific and Technical Information of China (English)

    白洪春; 白洪魁

    2012-01-01

    传统的逻辑思维是一种纵向的思维模式,有着使思维复杂化、片面化和对抗性的三大弊端。英国学者爱德华.德.波诺博士从平行思维的角度,开发的六顶思考帽思维模式,为人们提供了"平行思维"的工具,有效地避免了传统思维的弊端。运用六帽思维沟通方法具有能够避免沟通的对抗性、方法简捷使沟通具有高效性、分层次思维使沟通思路清晰、分解问题使沟通化繁为简、和谐情境使沟通增强团队创新能力、系统的观点使沟通的结论更全面和准确等优点,是值得推广的沟通方法。%Traditional logical thinking is a longitudinal mode of thinking,has three drawbacks of being complicated,one-sided and antagonistic.Ph.D Edward De Bono,a British scholar,developed six –hat thinking mode from the perspective of parallel thinking,providing people with a "parallel thinking tool to avoid the drawbacks of traditional thinking.This thinking method is able to avoid confrontation of communication,communicate ideas clearly and efficiently,simplify communication,create harmony situation,and enhance team innovation capability,offer more comprehensive and accurate conclusion.

  5. MRG15 activates the cdc2 promoter via histone acetylation in human cells

    Energy Technology Data Exchange (ETDEWEB)

    Pena, AndreAna N., E-mail: andreana.pena@gmail.com [Sam and Ann Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States); Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States); Tominaga, Kaoru; Pereira-Smith, Olivia M. [Sam and Ann Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States); Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX (United States)

    2011-07-01

    Chromatin remodeling is required for transcriptional activation and repression. MRG15 (MORF4L1), a chromatin modulator, is a highly conserved protein and is present in complexes containing histone acetyltransferases (HATs) as well as histone deacetylases (HDACs). Loss of expression of MRG15 in mice and Drosophila results in embryonic lethality and fibroblast and neural stem/progenitor cells cultured from Mrg15 null mouse embryos exhibit marked proliferative defects when compared with wild type cells. To determine the role of MRG15 in cell cycle progression we performed chromatin immunoprecipitation with an antibody to MRG15 on normal human fibroblasts as they entered the cell cycle from a quiescent state, and analyzed various cell cycle gene promoters. The results demonstrated a 3-fold increase in MRG15 occupancy at the cdc2 promoter during S phase of the cell cycle and a concomitant increase in acetylated histone H4. H4 lysine 12 was acetylated at 24 h post-serum stimulation while there was no change in acetylation of lysine 16. HDAC1 and 2 were decreased at this promoter during cell cycle progression. Over-expression of MRG15 in HeLa cells activated a cdc2 promoter-reporter construct in a dose-dependent manner, whereas knockdown of MRG15 resulted in decreased promoter activity. In order to implicate HAT activity, we treated cells with the HAT inhibitor anacardic acid and determined that HAT inhibition results in loss of expression of cdc2 mRNA. Further, chromatin immunoprecipitation with Tip60 localizes the protein to the same 110 bp stretch of the cdc2 promoter pulled down by MRG15. Additionally, we determined that cotransfection of MRG15 with the known associated HAT Tip60 had a cooperative effect in activating the cdc2 promoter. These results suggest that MRG15 is acting in a HAT complex involving Tip60 to modify chromatin via acetylation of histone H4 at the cdc2 promoter to activate transcription.

  6. Interaction of p21CDKN1A with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair

    Science.gov (United States)

    Cazzalini, Ornella; Perucca, Paola; Savio, Monica; Necchi, Daniela; Bianchi, Livia; Stivala, Lucia A.; Ducommun, Bernard; Scovassi, A. Ivana; Prosperi, Ennio

    2008-01-01

    The cell-cycle inhibitor p21CDKN1A has been suggested to directly participate in DNA repair, thanks to the interaction with PCNA. Yet, its role has remained unclear. Among proteins interacting with both p21 and PCNA, the histone acetyltransferase (HAT) p300 has been shown to participate in DNA repair. Here we report evidence indicating that p21 protein localizes and interacts with both p300 and PCNA at UV-induced DNA damage sites. The interaction between p300 and PCNA is regulated in vivo by p21. Indeed, loss of p21, or its inability to bind PCNA, results in a prolonged binding to chromatin and an increased association of p300 with PCNA, in UV-irradiated cells. Concomitantly, HAT activity of p300 is reduced after DNA damage. In vitro experiments show that inhibition of p300 HAT activity induced by PCNA is relieved by p21, which disrupts the association between recombinant p300 and PCNA. These results indicate that p21 is required during DNA repair to regulate p300 HAT activity by disrupting its interaction with PCNA. PMID:18263614

  7. Activation of p53 by spermine mediates induction of autophagy in HT1080 cells.

    Science.gov (United States)

    Chae, Yong-Byung; Kim, Moon-Moo

    2014-02-01

    The recent evidences indicate that autophagy is associated with a number of pathological processes including cancer, muscular disorder and neurodegeneration in addition to longevity. The efficacy of spermine was investigated on induction of autophagy through histone deacetylation and p53 activation in human fibrosarcoma cell line, HT1080. In this study, it was discovered that spermine increases the activity of HAT and autophagy. It was also identified that the transcriptional activation of p53 and the activation of p21 promoter by spermine are related to the induction of autophagy in reporter gene assay. Furthermore, western blot analyses demonstrated that spermine modulates the expression of proteins related to autophagy and apoptosis. The expression levels of Ac-histone H3, HDAC1, HAT1, p300 and SIRT1 were increased in HT1080 cells treated with spermine. In addition, the expression levels of protein such as acetyl-p53, p-p53, Bcl-2 and caspase-9 inducing apoptosis were increased in the presence of spermine. Moreover, the levels of Mdm2 and caspase-3 expression were reduced in the cells exposed to spermine compared to blank group. These results suggest that activation of HAT in the presence of spermine promotes the induction of autophagy in HT1080 cells through the enhanced activity of p-p53 and acetyl p53. Copyright © 2013 Elsevier B.V. All rights reserved.

  8. QSAR modeling for anti-human African trypanosomiasis activity of substituted 2-Phenylimidazopyridines

    Science.gov (United States)

    Masand, Vijay H.; El-Sayed, Nahed N. E.; Mahajan, Devidas T.; Mercader, Andrew G.; Alafeefy, Ahmed M.; Shibi, I. G.

    2017-02-01

    In the present work, sixty substituted 2-Phenylimidazopyridines previously reported with potent anti-human African trypanosomiasis (HAT) activity were selected to build genetic algorithm (GA) based QSAR models to determine the structural features that have significant correlation with the activity. Multiple QSAR models were built using easily interpretable descriptors that are directly associated with the presence or the absence of a structural scaffold, or a specific atom. All the QSAR models have been thoroughly validated according to the OECD principles. All the QSAR models are statistically very robust (R2 = 0.80-0.87) with high external predictive ability (CCCex = 0.81-0.92). The QSAR analysis reveals that the HAT activity has good correlation with the presence of five membered rings in the molecule.

  9. Operations Nougat and Whetstone events: Hard Hat, Danny Boy, Marshmallow, Mudpack, Wishbone, Gumdrop, Diluted Waters, and Tiny Tot, 15 February 1962-17 June 1965. Final report 15 Feb 62-19 Jun 68

    Energy Technology Data Exchange (ETDEWEB)

    Brady, W.J.; Horton, K.K.; Eubank, B.F.

    1984-01-31

    This report is a personnel oriented history of DOD participation in underground nuclear weapons testing during Operations NOUGAT and WHETSTONE, test events HARD HAT, DANNY BOY, MARSHMALLOW, MUDPACK, WISHBONE, GUMDROP, DILUTED WATERS, and TINY TOT. It is the first in a series of historical reports which will include all DOD underground nuclear weapons tests and DOE underground nuclear weapons tests with significant DOD participation from 1962 forward. In addition to these volumes presenting a history of the underground nuclear test program, a later restricted volume will identify all DOD participants, (military, civilian, and their contractors) and will list their dosimetry data.

  10. Gene panels to help identify subgroups at high and low risk of CHD among those randomized to antihypertensive treatment: The GenHAT Study

    Science.gov (United States)

    Lynch, Amy I.; Eckfeldt, John H.; Davis, Barry R.; Ford, Charles E.; Boerwinkle, Eric; Leiendecker-Foster, Catherine; Arnett, Donna K.

    2012-01-01

    Objective To identify panels of genetic variants that predict treatment-related coronary heart disease (CHD) outcomes in hypertensive patients on one of four different classes of initial antihypertensive treatment. The goal was to identify subgroups of people based on their genetic profile who benefit most from a particular treatment. Methods Candidate genetic variants (n=78) were genotyped in 39,114 participants from GenHAT, ancillary to ALLHAT. ALLHAT randomized hypertensive participants (>=55 years) to one of four treatments (amlodipine, chlorthalidone, doxazosin, lisinopril). The primary outcome was fatal CHD or non-fatal MI (mean follow-up=4.9 years). A pharmacogenetic panel was derived within each of the four treatment groups. ROC curves estimated the discrimination rate between those with and without a CHD event, based on the addition of the genetic panel risk score. Results For each treatment group, we identified a panel of genetic variants that collectively improved prediction of CHD to a small but statistically significant extent. Chlorthalidone (A): NOS3, rs3918226; SELE, rs5361; ICAM1, rs1799969; AGT, rs5051; GNAS, rs7121; ROC comparison p=.004; Amlodipine (B): MMP1, rs1799750; F5, rs6025; NPPA, rs5065; PDE4D, rs6450512; MMP9, rs2274756; ROC comparison p=.006; Lisinopril (C): AGT, rs5051; PON1, rs705379; MMP12, rs652438; F12, rs1801020; GP1BA, rs6065; PDE4D, rs27653; ROC comparison p=.01; Doxazosin (D): F2, rs1799963; PAI1, rs1799768; MMP7, rs11568818; AGT, rs5051; ACE, rs4343; MMP2, rs243865; ROC comparison p=.007. Each panel was tested for a pharmacogenetic effect; panels A, B and D showed such evidence (p=.009, .006, and .001 respectively), panel C did not (p=.09). Conclusion Because each panel was associated with CHD in a specific treatment group but not the others, this research provides evidence that it may be possible to use gene panel scores as a tool to better assess antihypertensive treatment choices to reduce CHD risk in hypertensive

  11. All Hat and No Cattle

    Science.gov (United States)

    Holloway-Libell, Jessica; Amrein-Beardsley, Audrey; Collins, Clarin

    2012-01-01

    Recently, two of the authors (Amrein-Beardsley & Collins, 2012) studied the impact of Education Value-Added Assessment System (EVAAS), a value-added model used to judge Houston teachers' performance. They examined the cases of four teachers who were terminated in summer 2011, at least in part because of their subpar EVAAS scores. Talking to…

  12. Wearing more than one hat?

    DEFF Research Database (Denmark)

    Høgedahl, Laust Kristian; Ibsen, Flemming

    , these multiple roles becomes problematic when public employers use the lockout weapon offensively in a combination with a state intervention to end the dispute as the case was during the teacher lockout in 2013. The article concludes with the presentation of a number of proposed adjustments for bringing......This article investigates the use of collective industrial conflict in the public sector by analysing the Danish teacher lockout in 2013. The social partners in the public sector in Denmark (and the other Scandinavian countries) engage in negotiations and reach agreements regarding wages....... But there is a big difference in the premises and conditions upon which collective industrial conflict proceeds when comparing the public and private sectors in Denmark. This article presents a critical analysis of the use of collective industrial conflicts in the Danish public sector in light of collective...

  13. All Hat and No Cattle

    Science.gov (United States)

    Holloway-Libell, Jessica; Amrein-Beardsley, Audrey; Collins, Clarin

    2012-01-01

    Recently, two of the authors (Amrein-Beardsley & Collins, 2012) studied the impact of Education Value-Added Assessment System (EVAAS), a value-added model used to judge Houston teachers' performance. They examined the cases of four teachers who were terminated in summer 2011, at least in part because of their subpar EVAAS scores. Talking to these…

  14. THE TRADE TIES HAT BIND

    Institute of Scientific and Technical Information of China (English)

    MATT; YOUNG

    2006-01-01

    Chinese President Hu Jintao made his first state visit to the United States April 19-21. The president kicked off his tour in Seattle, where he had intense contact with the American business community as represented by Boeing and Microsoft. Hu then landed in Washington D.C. for a summit meeting with President George W. Bush, followed by a visit to Yale University. During the meeting with Bush, Hu conveyed the idea that China and the United States are "not only stakeholders, but also constructive partners...

  15. Tarih Algısı Bağlamında Postmodern Bir Anlatı: İstanbul Hatırası A Postmodern Narration in the Context of History Perception: İstanbul Hatırası

    Directory of Open Access Journals (Sweden)

    Mustafa AYDEMİR

    2012-09-01

    Full Text Available Detective novel, formed around an intelligently planned murder and a detective who tries to solve it and find the murderer, shows a significant improvement with the influence of Postmodernism, which rose in the second half of 20th Century. That improvement first shows its face in translation, then writing in Turkish Literature. The history of detective novel, which began to take its place inliterary history due to the emergence of crime and along withurbanization, is as old as the concept of crime history. Having aparticular history, it can be claimed that it prepared the ground for theemergence of novel. However, two major war, which happened in the20th century, has led to a rapid development of the detective novel, thebest way to tell the story of the crimes.Ahmet Ümit’s novel İstanbul Hatırası is important as it shows thechange of detective novel in Turkish novel. A real – up to date and anunreal – historical, two different engaging fictions are being narrated inthat detective novel. Beginning with the murder, a journey to thehistorical sites of Istanbul is made. In addition, the importances ofthese sites, which are close to be destroyed, are pointed out.In this study, after mentioning how detective novel emerged inWest and Turkish Literature and its historic development, Ahmet Ümit’sİstanbul Hatırası has been analyzed and postmodern features in thisnovel has been emphasized. With this method, postmoderncharacteristics of the novel has been taken care in terms of plot, time,place, characters and the classical elements of the novel has beenevaluated with a postmodernist approach. Zekice plânlanmış bir cinayet ve onu çözmeye, katili bulmaya çalışan bir dedektif etrafında şekillenen polisiye roman, 20. yüzyılın ikinci yarısında ortaya çıkan postmodernizmin de etkisiyle önemli bir gelişme gösterir. Bu gelişme, Türk edebiyatında önce çeviri, sonra da telif yoluyla kendini gösterir. Suçun ortaya

  16. hatü’l-İnsân (Pend-Nâme-i Enûşîrvân’ın Türkçe Bir Tercümesi A Turkish Translation of Râhatü’l-İnsân (Pend-Nâme-i Enûşîrvân

    Directory of Open Access Journals (Sweden)

    Müjgân ÇAKIR

    2012-09-01

    Full Text Available Islamic literatures include many texts on the justice and counsels of Sassanian ruler Nûşîrevân. One of these is a work of Persian Literature which recounts the counsels written on his crown and figures with the titles of “Râhatü’l-insân” or “Pend-nâme-i Enûşîrvân” in some sources. Rıza Kulı Han Hidayet states that the text belongs to Bedâyi‛-i Belhî; however the identity of the author is disputed. It is also argued that the “Şerîf-i Şair,” whose name appears in the introduction to the text edited by Charles Schefer in his work entitled Chrestomathie Persane, is Ebû Şerîf Muhalledi-yi Gurgânî. Râhatü’l-insân has manuscripts in Turkish libraries. This article treats this work and its Turkish prose translation located at Süleymaniye Library, Fatih Section No. 5385 under the title “Pend-i Nûşîrevân-ı Âdil.” In this translation, the counsels written on the seventeen slices (küngüre of Nûşîrevân’s crown are given and it is told that this ruler had his crown put up somewhere appropriate every year for the soldiers to read his counsels. The purposes of Nûşîrevân’s counsels are proving a moral education to the public, directing his relations with his people as well as organizing the relations between them. Some of the counsels written on Nûşîrevân’s crown are as follows: “Respect the old and the weak, observe the rights of your parents, consult scholars before you do your business, have a command of your speech, do not betray trust, think before you speak.” The article will also treat some of the differences between the Persian manuscripts of the work and its Turkish translation. The transcription of the Turkish translation will be given. İslâmî edebiyatlarda Sasani hükümdarı Nûşîrevân’ın adaleti ve nasihatlarıyla ilgili birçok metin yazılmıştır. Bu metinlerden biri Fars Edebiyatı’nda onun tacının üzerinde yazılı olan nasihatları anlatan ve baz

  17. System parameters, transit times and secondary eclipse constraints of the exoplanet systems HAT-P-4, TrES-2, TrES-3 and WASP-3 from the NASA EPOXI Mission of Opportunity

    CERN Document Server

    Christiansen, Jessie L; Charbonneau, David; Deming, Drake; Holman, Matthew J; Madhusudhan, Nikku; Seager, Sara; Wellnitz, Dennis D; Barry, Richard K; Livengood, Timothy A; Hewagama, Tilak; Hampton, Don L; Lisse, Carey M; A'Hearn, Michael F

    2010-01-01

    As part of the NASA EPOXI Mission of Opportunity, we observed seven known transiting extrasolar planet systems in order to construct time series photometry of extremely high phase coverage and precision. Here we present the results for four "hot-Jupiter systems" with near-solar stars - HAT-P-4, TrES-3, TrES-2 and WASP-3. We observe ten transits of HAT-P-4, estimating the planet radius Rp = 1.332 \\pm 0.052 RJup, the stellar radius R \\star = 1.602 \\pm 0.061 R \\odot, the inclination i = 89.67 \\pm 0.30 degrees and the transit duration from first to fourth contact T = 255.6 \\pm 1.9 minutes. For TrES-3, we observe seven transits, and find Rp = 1.320 \\pm 0.057 RJup, R\\star = 0.817 \\pm 0.022 R\\odot, i = 81.99 \\pm 0.30 degrees and T = 81.9 \\pm 1.1 minutes. We also note a long term variability in the TrES-3 light curve, which may be due to star spots. We observe nine transits of TrES-2, and find Rp = 1.169 \\pm 0.034 RJup, R\\star = 0.940 \\pm 0.026 R\\odot, i = 84.15 \\pm 0.16 degrees and T = 107.3 \\pm 1.1 minutes. Finally...

  18. Grape seed extract regulates androgen receptor-mediated transcription in prostate cancer cells through potent anti-histone acetyltransferase activity.

    Science.gov (United States)

    Park, Si Yong; Lee, Yoo-Hyun; Choi, Kyung-Chul; Seong, Ah-Reum; Choi, Hyo-Kyoung; Lee, Ok-Hee; Hwang, Han-Joon; Yoon, Ho-Geun

    2011-01-01

    Histone acetylation, which is regulated by histone acetyltransferases (HATs) and deacetylases, is an epigenetic mechanism that influences eukaryotic transcription. Significant changes in histone acetylation are associated with cancer; therefore, manipulating the acetylation status of key gene targets is likely crucial for effective cancer therapy. Grape seed extract (GSE) has a known protective effect against prostate cancer. Here, we showed that GSE significantly inhibited HAT activity by 30-80% in vitro (P cancer cells by measuring luciferase activity using a pGL3-PSA construct bearing the AR element in the human prostate cancer cell line LNCaP (P cancer cell growth, and implicate GSE as a novel candidate for therapeutic activity against prostate cancer.

  19. Pharmacology of DB844, an orally active aza analogue of pafuramidine, in a monkey model of second stage human African trypanosomiasis.

    Directory of Open Access Journals (Sweden)

    John K Thuita

    Full Text Available Novel drugs to treat human African trypanosomiasis (HAT are still urgently needed despite the recent addition of nifurtimox-eflornithine combination therapy (NECT to WHO Model Lists of Essential Medicines against second stage HAT, where parasites have invaded the central nervous system (CNS. The pharmacology of a potential orally available lead compound, N-methoxy-6-{5-[4-(N-methoxyamidino phenyl]-furan-2-yl}-nicotinamidine (DB844, was evaluated in a vervet monkey model of second stage HAT, following promising results in mice. DB844 was administered orally to vervet monkeys, beginning 28 days post infection (DPI with Trypanosoma brucei rhodesiense KETRI 2537. DB844 was absorbed and converted to the active metabolite 6-[5-(4-phenylamidinophenyl-furanyl-2-yl]-nicotinamide (DB820, exhibiting plasma C(max values of 430 and 190 nM for DB844 and DB820, respectively, after the 14th dose at 6 mg/kg qd. A 100-fold reduction in blood trypanosome counts was observed within 24 h of the third dose and, at the end of treatment evaluation performed four days post the last drug dose, trypanosomes were not detected in the blood or cerebrospinal fluid of any monkey. However, some animals relapsed during the 300 days of post treatment monitoring, resulting in a cure rate of 3/8 (37.5% and 3/7 (42.9% for the 5 mg/kg×10 days and the 6 mg/kg×14 days dose regimens respectively. These DB844 efficacy data were an improvement compared with pentamidine and pafuramidine both of which were previously shown to be non-curative in this model of CNS stage HAT. These data show that synthesis of novel diamidines with improved activity against CNS-stage HAT was possible.

  20. Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate non-integrated iPSC reprogramming of human myeloid progenitors.

    Directory of Open Access Journals (Sweden)

    Tea Soon Park

    Full Text Available Nonviral conversion of skin or blood cells into clinically useful human induced pluripotent stem cells (hiPSC occurs in only rare fractions (~0.001%-0.5% of donor cells transfected with non-integrating reprogramming factors. Pluripotency induction of developmentally immature stem-progenitors is generally more efficient than differentiated somatic cell targets. However, the nature of augmented progenitor reprogramming remains obscure, and its potential has not been fully explored for improving the extremely slow pace of non-integrated reprogramming. Here, we report highly optimized four-factor reprogramming of lineage-committed cord blood (CB myeloid progenitors with bulk efficiencies of ~50% in purified episome-expressing cells. Lineage-committed CD33(+CD45(+CD34(- myeloid cells and not primitive hematopoietic stem-progenitors were the main targets of a rapid and nearly complete non-integrated reprogramming. The efficient conversion of mature myeloid populations into NANOG(+TRA-1-81(+ hiPSC was mediated by synergies between hematopoietic growth factor (GF, stromal activation signals, and episomal Yamanaka factor expression. Using a modular bioinformatics approach, we demonstrated that efficient myeloid reprogramming correlated not to increased proliferation or endogenous Core factor expressions, but to poised expression of GF-activated transcriptional circuits that commonly regulate plasticity in both hematopoietic progenitors and embryonic stem cells (ESC. Factor-driven conversion of myeloid progenitors to a high-fidelity pluripotent state was further accelerated by soluble and contact-dependent stromal signals that included an implied and unexpected role for Toll receptor-NFκB signaling. These data provide a paradigm for understanding the augmented reprogramming capacity of somatic progenitors, and reveal that efficient induced pluripotency in other cell types may also require extrinsic activation of a molecular framework that commonly

  1. Regulation of the histone acetyltransferase activity of hMOF via autoacetylation of Lys274

    Institute of Scientific and Technical Information of China (English)

    Bingfa Sun; Shunling Guo; Qingyu Tang; Chen Li; Rong Zeng; Zhiqi Xiong; Chen Zhong; Jianping Ding

    2011-01-01

    Dear Editor, Males-absent-on-the-first (MOF, also called MYST1 or KAT8) is a histone acetyltransferase (HAT) belonging to the MOZ, Ybf2/Sas3, Sas2 and Tip60 (MYST) family.MOF has been shown to possess a specific HAT activity towards Lysl6 of histone H4 (H4K16) [1].Homozygous knockout of MOF in mice results in loss of H4K16 acetylation and embryonic lethality, indicating that MOF and H4K16 acetylation are essential for embryogenesis and genome stability in mammals [2].Downregulation of human MOF (hMOF) leads to dramatic nuclear morphological deformation and inhibition of cell cycle progression [3], and has recently been correlated with primary breast carcinoma and medulloblastoma [4].

  2. CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes.

    Science.gov (United States)

    Ogiwara, Hideaki; Kohno, Takashi

    2012-01-01

    Histone acetylation at DNA double-strand break (DSB) sites by CBP and p300 histone acetyltransferases (HATs) is critical for the recruitment of DSB repair proteins to chromatin. Here, we show that CBP and p300 HATs also function in DSB repair by transcriptionally activating the BRCA1 and RAD51 genes, which are involved in homologous recombination (HR), a major DSB repair system. siRNA-mediated depletion of CBP and p300 impaired HR activity and downregulated BRCA1 and RAD51 at the protein and mRNA levels. Chromatin immunoprecipitation assays showed that CBP and p300 bind to the promoter regions of the BRCA1 and RAD51 genes, and that depletion of CBP and/or p300 reduces H3 and H4 acetylation and inhibits binding of the transcription factor E2F1 to these promoters. Depletion of CBP and p300 impaired DNA damage-induced phosphorylation and chromatin binding of the single-strand DNA-binding protein RPA following BRCA1-mediated DNA end resection. Consistent with this, subsequent phosphorylation of CHK1 and activation of the G2/M damage checkpoint were also impaired. These results indicate that the HATs CBP and p300 play multiple roles in the activation of the cellular response to DSBs.

  3. CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes.

    Directory of Open Access Journals (Sweden)

    Hideaki Ogiwara

    Full Text Available Histone acetylation at DNA double-strand break (DSB sites by CBP and p300 histone acetyltransferases (HATs is critical for the recruitment of DSB repair proteins to chromatin. Here, we show that CBP and p300 HATs also function in DSB repair by transcriptionally activating the BRCA1 and RAD51 genes, which are involved in homologous recombination (HR, a major DSB repair system. siRNA-mediated depletion of CBP and p300 impaired HR activity and downregulated BRCA1 and RAD51 at the protein and mRNA levels. Chromatin immunoprecipitation assays showed that CBP and p300 bind to the promoter regions of the BRCA1 and RAD51 genes, and that depletion of CBP and/or p300 reduces H3 and H4 acetylation and inhibits binding of the transcription factor E2F1 to these promoters. Depletion of CBP and p300 impaired DNA damage-induced phosphorylation and chromatin binding of the single-strand DNA-binding protein RPA following BRCA1-mediated DNA end resection. Consistent with this, subsequent phosphorylation of CHK1 and activation of the G2/M damage checkpoint were also impaired. These results indicate that the HATs CBP and p300 play multiple roles in the activation of the cellular response to DSBs.

  4. Diencephalic Size Is Restricted by a Novel Interplay Between GCN5 Acetyltransferase Activity and Retinoic Acid Signaling.

    Science.gov (United States)

    Wilde, Jonathan J; Siegenthaler, Julie A; Dent, Sharon Y R; Niswander, Lee A

    2017-03-08

    Diencephalic defects underlie an array of neurological diseases. Previous studies have suggested that retinoic acid (RA) signaling is involved in diencephalic development at late stages of embryonic development, but its roles and mechanisms of action during early neural development are still unclear. Here we demonstrate that mice lacking enzymatic activity of the acetyltransferase GCN5 ((Gcn5(hat/hat) )), which were previously characterized with respect to their exencephalic phenotype, exhibit significant diencephalic expansion, decreased diencephalic RA signaling, and increased diencephalic WNT and SHH signaling. Using a variety of molecular biology techniques in both cultured neuroepithelial cells treated with a GCN5 inhibitor and forebrain tissue from (Gcn5(hat/hat) ) embryos, we demonstrate that GCN5, RARα/γ, and the poorly characterized protein TACC1 form a complex in the nucleus that binds specific retinoic acid response elements in the absence of RA. Furthermore, RA triggers GCN5-mediated acetylation of TACC1, which results in dissociation of TACC1 from retinoic acid response elements and leads to transcriptional activation of RA target genes. Intriguingly, RA signaling defects caused by in vitro inhibition of GCN5 can be rescued through RA-dependent mechanisms that require RARβ. Last, we demonstrate that the diencephalic expansion and transcriptional defects seen in (Gcn5(hat/hat) ) mutants can be rescued with gestational RA supplementation, supporting a direct link between GCN5, TACC1, and RA signaling in the developing diencephalon. Together, our studies identify a novel, nonhistone substrate for GCN5 whose modification regulates a previously undescribed, tissue-specific mechanism of RA signaling that is required to restrict diencephalic size during early forebrain development.SIGNIFICANCE STATEMENT Changes in diencephalic size and shape, as well as SNPs associated with retinoic acid (RA) signaling-associated genes, have been linked to neuropsychiatric

  5. The Influence of Peripheral Substituent Modification on P(V), Mn(III), and Mn(V)(O) Corrolazines: X-ray Crystallography, Electrochemical and Spectroscopic Properties, and HAT and OAT Reactivities.

    Science.gov (United States)

    Joslin, Evan E; Zaragoza, Jan Paulo T; Baglia, Regina A; Siegler, Maxime A; Goldberg, David P

    2016-09-06

    The influence of remote peripheral substitution on the physicochemical properties and reactivity of phosphorus and manganese corrolazine (Cz) complexes was examined. The substitution of p-MeO for p-t-Bu groups on the eight phenyl substituents of the β-carbon atoms of the Cz ring led to changes in UV-vis transitions and redox potentials for each of the complexes. The oxygen atom transfer (OAT) and hydrogen atom transfer (HAT) reactivity of the Mn(V)(O) complexes was also influenced by p-MeO substitution. The OAT reactivity of Mn(V)(O)(MeOP8Cz) (MeOP8Cz = octakis(p-methoxyphenyl)corrolazinato(3-)) with triarylphosphine (PAr3) substrates led to second-order rate constants from 10.2(5) to 3.1(2) × 10(4) M(-1) s(-1). These rates of OAT are slower than those seen for Mn(V)(O)(TBP8Cz) (TBP8Cz = octakis(p-tert-butylphenyl)corrolazinato(3-)). A Hammett study involving para-substituted PAr3 substrates reveals a Hammett ρ-value for Mn(V)(O)(MeOP8Cz) that is more negative than that observed for Mn(V)(O)(TBP8Cz), consistent with a less electrophilic Mn center. The HAT reactivity of Mn(V)(O)(MeOP8Cz) with C-H substrates was examined and revealed second-order rate constants from 6.8(5) × 10(-5) to 1.70(2) × 10(-1) M(-1) s(-1). The rate constants varied with the C-H bond strength of the substrate. Slightly faster HAT rates with C-H substrates were observed with Mn(V)(O)(MeOP8Cz) compared to Mn(V)(O)(TBP8Cz), indicating that the basicity of the putative [Mn(IV)(O)](-) intermediate likely compensates for the more negative redox potential in the driving force for HAT. In addition, the complete, large-scale synthesis of the para-phenyl-substituted porphyrazines RP8PzH2 (R = p-tert-butylphenyl (TB), p-methoxyphenyl (MeO), and p-isopropylphenyl) and corrolazines RP8CzH3 (TBP8CzH3 and MeOP8CzH3) is presented. The crystal structures of the monoprotonated, metal-free corrolazine [(TBP8CzH3)(H)](+)[BArF](-), P(V)(OMe)2(MeOP8Cz), and Mn(III)(MeOP8Cz)(MeOH) are presented. This work

  6. 论《麦田里的守望者》中霍尔顿帽子的象征意义%Symbolic meaning of Holden Caulfield' s hat in The Catcher in the Rye

    Institute of Scientific and Technical Information of China (English)

    孙珂

    2012-01-01

    Symbolism is extensively employed in hterary writings to convey some particular impheation that is hidden in the context but fully embodies the theme of the work. Salinger employs the device of symbohsm in his The Catcher in the Rye to reflect the emptiness and painfulness of teenager' s sate of mind,which is in radical contrast with the economic prosperity after the Second World War in the U. S. A. In this paper,the color,the peak,the name of the hat,and the time Holden wears it are carefully examined to cast light on the symbulic meaning of a red hunting hat,a small item that accompanies Holden Caulfield in the short period of his initiation.%在文学创作中,象征作为一种隐晦的表现手法被广泛用来深化作品的主题,塞林格在其代表作《麦田里的守望者》一书中运用这一手法,含蓄地反映了美国六十年代经济繁荣背后人们内心的空虚,特别是青少年成长过程中的迷惘心态。通过对小说中伴随主人公霍尔顿成长的猎帽的名字、颜色、鸭舌和霍尔顿佩戴猎帽的时间进行详细分析,阐述了《麦田里的守望者》中猎帽所蕴含的象征意义。

  7. A Theoretical Study on the Antioxidant Activity of Piceatannol and Isorhapontigenin Scavenging Nitric Oxide and Nitrogen Dioxide Radicals

    Science.gov (United States)

    Lu, Yang; Wang, AiHua; Shi, Peng; Zhang, Hui

    2017-01-01

    The antioxidant activity of naturally occurring stilbene compounds piceatannol (PIC) and isorhapontigenin (ISO) scavenging two free radicals (NO and NO2) were studied using density functional theory (DFT) method. Four reaction mechanisms have been considered: hydrogen atom transfer (HAT), radical adduct formation (RAF), single electron transfer (SET), and sequential proton loss electron transfer (SPLET). The reaction channels in water solution were traced independently, and the respective thermodynamic and kinetic parameters were obtained. We found PIC and ISO scavenge NO mainly through RAF mechanism, and scavenge NO2 through HAT mechanism. The capacity of PIC scavenging NO2 is much higher than ISO, but the reactivity of scavenging NO is lower than ISO. PMID:28068377

  8. SCYX-7158, an orally-active benzoxaborole for the treatment of stage 2 human African trypanosomiasis.

    Directory of Open Access Journals (Sweden)

    Robert T Jacobs

    2011-06-01

    Full Text Available BACKGROUND: Human African trypanosomiasis (HAT is an important public health problem in sub-Saharan Africa, affecting hundreds of thousands of individuals. An urgent need exists for the discovery and development of new, safe, and effective drugs to treat HAT, as existing therapies suffer from poor safety profiles, difficult treatment regimens, limited effectiveness, and a high cost of goods. We have discovered and optimized a novel class of small-molecule boron-containing compounds, benzoxaboroles, to identify SCYX-7158 as an effective, safe and orally active treatment for HAT. METHODOLOGY/PRINCIPAL FINDINGS: A drug discovery project employing integrated biological screening, medicinal chemistry and pharmacokinetic characterization identified SCYX-7158 as an optimized analog, as it is active in vitro against relevant strains of Trypanosoma brucei, including T. b. rhodesiense and T. b. gambiense, is efficacious in both stage 1 and stage 2 murine HAT models and has physicochemical and in vitro absorption, distribution, metabolism, elimination and toxicology (ADMET properties consistent with the compound being orally available, metabolically stable and CNS permeable. In a murine stage 2 study, SCYX-7158 is effective orally at doses as low as 12.5 mg/kg (QD×7 days. In vivo pharmacokinetic characterization of SCYX-7158 demonstrates that the compound is highly bioavailable in rodents and non-human primates, has low intravenous plasma clearance and has a 24-h elimination half-life and a volume of distribution that indicate good tissue distribution. Most importantly, in rodents brain exposure of SCYX-7158 is high, with C(max >10 µg/mL and AUC(0-24 hr >100 µg*h/mL following a 25 mg/kg oral dose. Furthermore, SCYX-7158 readily distributes into cerebrospinal fluid to achieve therapeutically relevant concentrations in this compartment. CONCLUSIONS/SIGNIFICANCE: The biological and pharmacokinetic properties of SCYX-7158 suggest that this compound will be

  9. Complexes of ruthenium(II) with bpmRe(CO) sub 3 Cl and HAT(Re(CO) sub 3 Cl) sub 2 as ligands: Syntheses and redox and luminescence properties

    Energy Technology Data Exchange (ETDEWEB)

    Sahai, R.; Rillema, D.P.; Shaver, R.; Van Wallendael, S.; Jackman, D.C.; Boldaji, M. (Univ. of North Carolina, Charlotte (USA))

    1989-03-22

    A series of mixed-metal complexes containing ruthenium and rhenium are reported. The new complexes are ((bpy)Ru-(bpmRe(CO){sub 3}Cl){sub 2})(PF{sub 6}){sub 2}, ((bpy){sub 2}RuHAT(Re(CO){sub 3}Cl){sub 2})(PF{sub 6}){sub 2}, and (Ru(bpmRe(CO){sub 3}Cl){sub 3})(PF{sub 6}){sub 2}. Optical transitions in the visible-uv spectrum are dominated by the presence of the ruthenium heterocycles that have absorption coefficients for the low-energy transition 1 order of magnitude greater than that of the rhenium chromophore. The low-energy transition blue-shifts in the series ((bpy){sub 2}Ru(bpmRe(CO){sub 3}Cl)){sup 2+} (558 nm) < ((bpy)Ru(bpmRe(CO){sub 3}Cl){sub 2}){sup 2+} (531 nm) < (Ru(bpmRe(CO){sub 3}Cl){sub 3}){sup 2+} (501 nm). Polarographic half-wave potentials of the metal-centered oxidations and the bridging-ligand reductions shift positively as the number of Re(CO){sub 3}Cl units increase. The E{sub {1/2}} values for the ((bpy){sub n}Ru(bpmRe(CO){sub 3}Cl){sub 3-n}){sup 2+/+} couple in the series with n = 0-2 were -0.1, -0.33, and -0.41 V vs SSCE, respectively. Two complexes ((bpy)Ru(bpmRe(CO){sub 3}Cl){sub 2}){sup 2+} and ((Ru-(bpmRe(CO){sub 3}Cl){sub 3})){sup 2+}, were found to luminesce weakly at room temperature in acetonitrile with lifetimes of 942 and 847 ns, respectively. In many respects, the mixed-metal complexes are shown to behave as if the bpmRe(CO){sub 3}Cl and HAT(Re(CO){sub 3}Cl){sub 2} chromophores are ligands coordinated to ruthenium(II). 17 refs., 8 figs., 4 tabs.

  10. Piceatannol and resveratrol share inhibitory effects on hydrogen peroxide release, monoamine oxidase and lipogenic activities in adipose tissue, but differ in their antilipolytic properties.

    Science.gov (United States)

    Les, Francisco; Deleruyelle, Simon; Cassagnes, Laure-Estelle; Boutin, Jean A; Balogh, Balázs; Arbones-Mainar, José M; Biron, Simon; Marceau, Picard; Richard, Denis; Nepveu, Françoise; Mauriège, Pascale; Carpéné, Christian

    2016-10-25

    Piceatannol is a hydroxylated derivative of resveratrol. While both dietary polyphenols coexist in edible plants and fruits, and share equivalent concentrations in several wines, the influence of piceatannol on adiposity has been less studied than that of resveratrol. Though resveratrol is now recognized to limit fat deposition in various obesity models, the benefit of its dietary supplementation remains under debate regarding human obesity treatment or prevention. The research for more potent resveratrol analogs is therefore still undergoing. This prompted us to compare various effects of piceatannol and resveratrol directly on human adipose tissue (hAT). Hydrogen peroxide release was measured by Amplex Red-based fluorescence in subcutaneous hAT samples from obese patients. Interactions of stilbenes with human amine oxidases and quinone reductase were assessed by radiometric methods, computational docking and electron paramagnetic resonance. Influences on lipogenic and lipolytic activities were compared in mouse adipocytes. Resveratrol and piceatannol inhibited monoamine oxidase (MAO) with respective IC50 of 18.5 and 133.7 μM, but not semicarbazide-sensitive amine oxidase (SSAO) in hAT. For both stilbenes, the docking scores were better for MAO than for SSAO. Piceatannol and resveratrol similarly hampered hydrogen peroxide detection in assays with and without hAT, while they shared pro-oxidant activities when incubated with purified quinone reductase. They exhibited similar dose-dependent inhibition of adipocyte lipogenic activity. Only piceatannol inhibited basal and stimulated lipolysis when incubated at a dose ≥100 μM. Thus, piceatannol exerted on fat cells dose-dependent effects similar to those of resveratrol, except for a stronger antilipolytic action. In this regard, piceatannol should be useful in limiting the lipotoxicity related to obesity when ingested or administered alone - or might hamper the fat mobilization induced by resveratrol when

  11. Activation Induces Structural Changes in the Liganded Angiotensin II Type 1 Receptor*

    Science.gov (United States)

    Clément, Martin; Cabana, Jérôme; Holleran, Brian J.; Leduc, Richard; Guillemette, Gaétan; Lavigne, Pierre; Escher, Emanuel

    2009-01-01

    The octapeptide hormone angiotensin II (AngII) binds to and activates the human angiotensin II type 1 receptor (hAT1) of the G protein-coupled receptor class A family. Several activation mechanisms have been proposed for this family, but they have not yet been experimentally validated. We previously used the methionine proximity assay to show that 11 residues in transmembrane domain (TMD) III, VI, and VII of the hAT1 receptor reside in close proximity to the C-terminal residue of AngII. With the exception of a single change in TMD VI, the same contacts are present on N111G-hAT1, a constitutively active mutant; this N111G-hAT1 is a model for the active form of the receptor. In this study, two series of 53 individual methionine mutations were constructed in TMD I, II, IV, and V on both receptor forms. The mutants were photolabeled with a neutral antagonist, 125I-[Sar1,p-benzoyl-l-Phe8]AngII, and the resulting complexes were digested with cyanogen bromide. Although no new contacts were found for the hAT1 mutants, two were found in the constitutively active mutants, Phe-77 in TMD II and Asn-200 in TMD V. To our knowledge, this is the first time that a direct ligand contact with TMD II and TMD V has been reported. These contact point differences were used to identify the structural changes between the WT-hAT1 and N111G-hAT1 complexes through homology-based modeling and restrained molecular dynamics. The model generated revealed an important structural rearrangement of several TMDs from the basal to the activated form in the WT-hAT1 receptor. PMID:19635801

  12. Spitzer Secondary Eclipse Depths with Multiple Intrapixel Sensitivity Correction Methods: Observations of WASP-13b, WASP-15b, WASP-16b, WASP-62b, and HAT-P-22b

    CERN Document Server

    Kilpatrick, Brian M; Kataria, Tiffany; Deming, Drake; Ingalls, James G; Krick, Jessica E; Tucker, Gregory S

    2016-01-01

    We measure the 4.5 $\\mu$m thermal emission of five transiting hot Jupiters, WASP-13b, WASP-15b, WASP-16b, WASP-62b and HAT-P-22b using channel 2 of the Infrared Array Camera (IRAC) on the {\\sl Spitzer Space Telescope}. Significant intrapixel sensitivity variations in Spitzer IRAC data require careful correction in order to achieve precision on the order of several hundred parts per million (ppm) for the measurement of exoplanet secondary eclipses. We determine eclipse depths by first correcting the raw data using three independent data reduction methods. The Pixel Gain Map (PMAP), Nearest Neighbors (NNBR), and Pixel Level Decorrelation (PLD) each correct for the intrapixel sensitivity effect in Spitzer photometric time-series observations. The results from each methodology are compared against each other to establish if they reach a statistically equivalent result in every case and to evaluate their ability to minimize uncertainty in the measurement. We find that all three methods produce reliable results. Fo...

  13. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species

    Directory of Open Access Journals (Sweden)

    Piotr Michel

    2012-03-01

    Full Text Available The antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer and HAT-type (hydrogen atom transfer mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and TroloxÒ. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH (EC50 = 3.2–5.2 μg/mL, TEAC (2.8–4.0 mmol Trolox®/g, and FRAP (9.8–13.7 mmol Fe2+/g tests, and strongly correlated with the total phenolic levels (39.6–58.2% of gallic acid equivalents. The HPLC-PDA analysis of the extracts led to the identification of chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and sexangularetin 3-O-b-D-glucopyranoside as the main components. Apart from flavonoids and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the SET-type activity. The HAT-reactivity of the extracts in the linoleic acid peroxidation test (IC50 = 36.9–228.3 μg/mL depended more strongly on the plant tissue than on the extraction solvent, and its correlation with the phenolic content was weak. Both SET and HAT-type activity of the most potent Sorbus extracts was comparable with the activity of the standards, indicating their great potential as effective sources for health products.

  14. Mechanistic Variants in Gas-Phase Metal-Oxide Mediated Activation of Methane at Ambient Conditions.

    Science.gov (United States)

    Li, Jilai; Zhou, Shaodong; Zhang, Jun; Schlangen, Maria; Usharani, Dandamudi; Shaik, Sason; Schwarz, Helmut

    2016-09-07

    The C-H bond activation of methane mediated by a prototypical heteronuclear metal-oxide cluster, [Al2Mg2O5](•+), was investigated by using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) in conjunction with high-level quantum mechanical calculations. Experimentally, hydrogen-atom abstraction from methane by the cluster ion [Al2Mg2O5](•+) takes place at ambient conditions. As to the mechanism, according to our computational findings, both the proton-coupled electron transfer (PCET) and the conventional hydrogen-atom transfer (HAT) are feasible and compete with each other. This is in distinct contrast to the [XYO2](+) (X, Y = Mg, Al, Si) cluster oxide ions which activate methane exclusively via the PCET route (Li, J.; Zhou, S.; Zhang, J.; Schlangen, M.; Weiske, T.; Usharani, D.; Shaik, S.; Schwarz, H. J. Am. Chem. Soc. 2016, 138, 7973-7981). The electronic origins of the mechanistically rather complex reactivity scenarios of the [Al2Mg2O5](•+)/CH4 couple were elucidated. For the PCET mechanism, in which the Lewis acid-base pair [Al(+)-O(-)] of the cluster acts as the active site, a clear correlation has been established between the nature of the transition state, the corresponding barrier height, the Lewis acidity-basicity of the [M(+)-O(-)] unit, as well as the bond order of the M(+)-O(-) bond. Also addressed is the role of the spin and charge distributions of a terminal oxygen radical site in the direct HAT route. The knowledge of the factors that control the reactivity of PCET and HAT pathways not only deepens our mechanistic understanding of metal-oxide mediated C-H bond activation but may also provide guidance for the rational design of catalysts.

  15. Acetylation of retinal histones in diabetes increases inflammatory proteins: effects of minocycline and manipulation of histone acetyltransferase (HAT) and histone deacetylase (HDAC).

    Science.gov (United States)

    Kadiyala, Chandra Sekhar Rao; Zheng, Ling; Du, Yunpeng; Yohannes, Elizabeth; Kao, Hung-Ying; Miyagi, Masaru; Kern, Timothy S

    2012-07-27

    Histone acetylation was significantly increased in retinas from diabetic rats, and this acetylation was inhibited in diabetics treated with minocycline, a drug known to inhibit early diabetic retinopathy in animals. Histone acetylation and expression of inflammatory proteins that have been implicated in the pathogenesis of diabetic retinopathy were increased likewise in cultured retinal Müller glia grown in a diabetes-like concentration of glucose. Both the acetylation and induction of the inflammatory proteins in elevated glucose levels were significantly inhibited by inhibitors of histone acetyltransferase (garcinol and antisense against the histone acetylase, p300) or activators of histone deacetylase (theophylline and resveratrol) and were increased by the histone deacetylase inhibitor, suberolylanilide hydroxamic acid. We conclude that hyperglycemia causes acetylation of retinal histones (and probably other proteins) and that the acetylation contributes to the hyperglycemia-induced up-regulation of proinflammatory proteins and thereby to the development of diabetic retinopathy.

  16. Histone Acetyltransferase Activity of MOF Is Required for MLL-AF9 Leukemogenesis.

    Science.gov (United States)

    Valerio, Daria G; Xu, Haiming; Chen, Chun-Wei; Hoshii, Takayuki; Eisold, Meghan E; Delaney, Christopher; Cusan, Monica; Deshpande, Aniruddha J; Huang, Chun-Hao; Lujambio, Amaia; Zheng, YuJun George; Zuber, Johannes; Pandita, Tej K; Lowe, Scott W; Armstrong, Scott A

    2017-02-15

    Chromatin-based mechanisms offer therapeutic targets in acute myeloid leukemia (AML) that are of great current interest. In this study, we conducted an RNAi-based screen to identify druggable chromatin regulator-based targets in leukemias marked by oncogenic rearrangements of the MLL gene. In this manner, we discovered the H4K16 histone acetyltransferase (HAT) MOF to be important for leukemia cell growth. Conditional deletion of Mof in a mouse model of MLL-AF9-driven leukemogenesis reduced tumor burden and prolonged host survival. RNA sequencing showed an expected downregulation of genes within DNA damage repair pathways that are controlled by MOF, as correlated with a significant increase in yH2AX nuclear foci in Mof-deficient MLL-AF9 tumor cells. In parallel, Mof loss also impaired global H4K16 acetylation in the tumor cell genome. Rescue experiments with catalytically inactive mutants of MOF showed that its enzymatic activity was required to maintain cancer pathogenicity. In support of the role of MOF in sustaining H4K16 acetylation, a small-molecule inhibitor of the HAT component MYST blocked the growth of both murine and human MLL-AF9 leukemia cell lines. Furthermore, Mof inactivation suppressed leukemia development in an NUP98-HOXA9-driven AML model. Taken together, our results establish that the HAT activity of MOF is required to sustain MLL-AF9 leukemia and may be important for multiple AML subtypes. Blocking this activity is sufficient to stimulate DNA damage, offering a rationale to pursue MOF inhibitors as a targeted approach to treat MLL-rearranged leukemias. Cancer Res; 77(7); 1-10. ©2017 AACR.

  17. Sex difference and menstrual cycle effects on semantic priming and rememberingAnlamsal hazırlama ve hatırlamada cinsiyet farkı ve menstural dönem etkileri

    Directory of Open Access Journals (Sweden)

    Evrim Gülbetekin

    2015-02-01

    fluctuate across the menstrual cycle. The findings were discussed in the context of the negative effects of increasing luteinizing hormone (LH levels on attentional and executive functions in both ovulatory phase and Alzheimer’s disease. ÖzetAmaç: Kadınların ve erkeklerin bazı bilişsel işlemlerde ve hemisferik özelleşmelerde farklılık gösterdiği; buna karşın, cinsiyet farklılıklarına ilişkin çalışmaların her zaman çok tutarlı olmadığı bilinmektedir. Son dönemlerde yapılan çalışmalar, kadınlardaki bilişsel becerilerin menstural döneme bağlı olarak dalgalanma gösterebildiğine işaret etmiştir. Sunulan çalışmada erkeklerin ve farklı menstural dönemdeki kadınların (yumurtlama ve mensturasyon anlamsal hazırlama (AH ve hatırlama görevindeki peformansları yönünden farklılık gösterip göstermediğini incelemek amaçlanmıştır. Ayrıca AH görevinde cinsiyete özgü hemisferik özelleşme olup olmadığı incelenmiştir.Yöntem: Çalışmaya 72 kadın ve 29 erkek üniversite öğrencisi katılmıştır. AH görevi için katılımcılara arka arkaya iki resim gösterilmiştir. Görsel-yarı alan tekniği kullanılarak ve deney gruplarına uygun olarak ilk resim sol görsel alanda, sağ görsel alanda ya da merkezde100ms sunulmuş; katılımcılardan merkezde sunulan birinci resimle ikinci resmin ilişkili olup olmadığını belirtmeleri istenmiştir. AH görevinin ardından katılımcılara deneydeki uyarıcıların bir listesi verilmiş ve bunun içinden hatırladıklarını bir dakikalık sürede işartlemeleri istenmiştir.Bulgular: Tüm gruplarda AH etkisinin olduğu; ancak bu göreve ilişkin bir hemisferik özelleşmenin olmadığı bulunmuştur. Erkekler ilişkili resimleri yumurtlama dönemindeki kadınlardan daha hızlı değerlendirirken, mensturasyon dönemindeki kadınlarla böyle bir farklılık gözlenmemiştir. Benzer şekilde erkeklerin hatırladıkları genel uyarıcı ve ilişkisiz uyarıcı say

  18. 基于顶帽-底帽变换和二次图像分割的目标提取%Target extraction based on the hat-bottom cap transformation and second image segmentation

    Institute of Scientific and Technical Information of China (English)

    杨漫

    2016-01-01

    This paper use the method of maximum amount of variance , iterative , two-dimensional maximum entropy , KFCM single threshold segmentation and grayscale average region to put the tanks and trucks segmented from the background growing respectively. To solve the problem of single threshold segmentation method and the grayscale average region growing method being not able to segment the car or lost some part in extracting target projection , this paper has combined the advantage of single threshold segmentation method and the region growing method , and has put forward a target extraction method based on the hat-bottom cap transformation and the second image segmentation. Comparison test shows that the algorithm can remove the bodywork projection while remaining the intact body , which can inhibit the target centroid drift to a large extent.%文中分别采用最大内间方差法、迭代法、二维最大熵法、KFCM单次阈值分割法和灰度均值区域生长法对坦克和卡车从背景中进行提取。针对单次阈值分割法和灰度均值区域生长法在提取目标时出现不能分割出车身投影或者大量丢失车体等问题,本文结合单次阈值分割法和区域生长法的优势,提出了基于顶帽-底帽变换和二次图像分割的目标提取算法。对比试验表明,该算法在较完整保留车体部分同时能去除车体的投影,很大程度上抑制了目标的形心漂移。

  19. Calcium in pollen-pistil interaction in `Petunia hybrida Hat`. Pt. 3. Localization of Ca{sup 2+} ions and Ca{sup 2+}-ATPase in pollinated pistil

    Energy Technology Data Exchange (ETDEWEB)

    Bednarska, E.; Butowt, R. [Uniwersytet Mikolaja Kopernika, Torun (Poland)

    1995-12-31

    Studies were carried out of Ca{sup 2+} and Ca{sup 2+}-ATPase localization in pollinated (6 and 48 h after pollination) pistils of `Petunia hybrida`. The results were confronted with Ca{sup 2+} localization in mature pollen grain and in unpollinated pistil. It has been found that after pollination the number of Ca{sup 2+} sequestered in the stigmal exudate and in the sporoderm of the pollen grain gets lower. That phenomenon was associated with the appearance of a large number of Sb/Ca precipitates in the submembrane cytoplasm of the germinating pollen. In the vacuolized pollen grain, i.e. grown into a pollen tube, there were only a few precipitates. In the pollen tube, Ca{sup 2+} were found in the organelles of the tip cytoplasm and in the external pectin cell wall. Studies with the use of {sup 45}Ca{sup 2+} have revealed that the source of calcium ions incorporated into the pollen tube tip and its pectin wall is the transmitting tract of the style. In the transmitting tract overgrown with pollen tubes, Ca{sup 2+} were localized in the intercellular matrix and in the transmitting cell. Sb/Ca precipitates occurred in the nuclei, around the secretary vesicles and on the plasmalemma in the transverse walls region. Elevated Ca{sup 2+} level was found in degenerating cells (inhibited pollen tubes, transmitting cells, nucellar cells). The progressing degeneration process of the cells of the transmitting tract of the pollinated pistil was associated with a decrease in the activity of plasmalemma Ca{sup 2+}-ATPase. (author). 30 refs, 19 figs.

  20. Structure-thermodynamics-antioxidant activity relationships of selected natural phenolic acids and derivatives: an experimental and theoretical evaluation.

    Directory of Open Access Journals (Sweden)

    Yuzhen Chen

    Full Text Available Phenolic acids and derivatives have potential biological functions, however, little is known about the structure-activity relationships and the underlying action mechanisms of these phenolic acids to date. Herein we investigate the structure-thermodynamics-antioxidant relationships of 20 natural phenolic acids and derivatives using DPPH• scavenging assay, density functional theory calculations at the B3LYP/6-311++G(d,p levels of theory, and quantitative structure-activity relationship (QSAR modeling. Three main working mechanisms (HAT, SETPT and SPLET are explored in four micro-environments (gas-phase, benzene, water and ethanol. Computed thermodynamics parameters (BDE, IP, PDE, PA and ETE are compared with the experimental radical scavenging activities against DPPH•. Available theoretical and experimental investigations have demonstrated that the extended delocalization and intra-molecular hydrogen bonds are the two main contributions to the stability of the radicals. The C = O or C = C in COOH, COOR, C = CCOOH and C = CCOOR groups, and orthodiphenolic functionalities are shown to favorably stabilize the specific radical species to enhance the radical scavenging activities, while the presence of the single OH in the ortho position of the COOH group disfavors the activities. HAT is the thermodynamically preferred mechanism in the gas phase and benzene, whereas SPLET in water and ethanol. Furthermore, our QSAR models robustly represent the structure-activity relationships of these explored compounds in polar media.