WorldWideScience

Sample records for actinium 223

  1. Production of high-purity radium-223 from legacy actinium-beryllium neutron sources.

    Science.gov (United States)

    Soderquist, Chuck Z; McNamara, Bruce K; Fisher, Darrell R

    2012-07-01

    Radium-223 is a short-lived alpha-particle-emitting radionuclide with potential applications in cancer treatment. Research to develop new radiopharmaceuticals employing (223)Ra has been hindered by poor availability due to the small quantities of parent actinium-227 available world-wide. The purpose of this study was to develop innovative and cost-effective methods to obtain high-purity (223)Ra from (227)Ac. We obtained (227)Ac from two surplus actinium-beryllium neutron generators. We retrieved the actinium/beryllium buttons from the sources and dissolved them in a sulfuric-nitric acid solution. A crude actinium solid was recovered from the solution by coprecipitation with thorium fluoride, leaving beryllium in solution. The crude actinium was purified to provide about 40 milligrams of actinium nitrate using anion exchange in methanol-water-nitric acid solution. The purified actinium was then used to generate high-purity (223)Ra. We extracted (223)Ra using anion exchange in a methanol-water-nitric acid solution. After the radium was separated, actinium and thorium were then eluted from the column and dried for interim storage. This single-pass separation produces high purity, carrier-free (223)Ra product, and does not disturb the (227)Ac/(227)Th equilibrium. A high purity, carrier-free (227)Th was also obtained from the actinium using a similar anion exchange in nitric acid. These methods enable efficient production of (223)Ra for research and new alpha-emitter radiopharmaceutical development.

  2. Discovery of the actinium, thorium, protactinium, and uranium isotopes

    CERN Document Server

    Fry, C

    2012-01-01

    Currently, 31 actinium, 31 thorium, 28 protactinium, and 23 uranium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  3. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-01-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac–Cl and Ac–OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1– ligands (3.2±1.1) than AmIII (0.8±0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour. PMID:27531582

  4. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-08-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac-Cl and Ac-OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1- ligands (3.2+/-1.1) than AmIII (0.8+/-0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour.

  5. Relativistic small-core pseudopotentials for actinium, thorium, and protactinium.

    Science.gov (United States)

    Weigand, Anna; Cao, Xiaoyan; Hangele, Tim; Dolg, Michael

    2014-04-03

    Small-core pseudopotentials for actinium, thorium, and protactinium have been energy-adjusted to multiconfiguration Dirac-Hartree-Fock reference data based on the Dirac-Coulomb-Breit Hamiltonian and the Fermi nucleus model. Corresponding optimized valence basis sets of polarized valence quadruple-ζ quality are presented. Atomic test calculations for the first four ionization potentials show satisfactory results at both the Hartree-Fock and the multireference averaged coupled-pair functional level. Highly correlated Fock-space coupled cluster calculations demonstrate that the new pseudopotentials yield ionization potentials, which are in excellent agreement with corresponding all-electron results and experimental data. The pseudopotentials and basis sets supplement a similar set previously published for uranium.

  6. Production of Actinium-225 via High Energy Proton Induced Spallation of Thorium-232

    Energy Technology Data Exchange (ETDEWEB)

    Harvey, James T.; Nolen, Jerry; Vandergrift, George; Gomes, Itacil; Kroc, Tom; Horwitz, Phil; McAlister, Dan; Bowers, Del; Sullivan, Vivian; Greene, John

    2011-12-30

    The science of cancer research is currently expanding its use of alpha particle emitting radioisotopes. Coupled with the discovery and proliferation of molecular species that seek out and attach to tumors, new therapy and diagnostics are being developed to enhance the treatment of cancer and other diseases. This latest technology is commonly referred to as Alpha Immunotherapy (AIT). Actinium-225/Bismuth-213 is a parent/daughter alpha-emitting radioisotope pair that is highly sought after because of the potential for treating numerous diseases and its ability to be chemically compatible with many known and widely used carrier molecules (such as monoclonal antibodies and proteins/peptides). Unfortunately, the worldwide supply of actinium-225 is limited to about 1,000mCi annually and most of that is currently spoken for, thus limiting the ability of this radioisotope pair to enter into research and subsequently clinical trials. The route proposed herein utilizes high energy protons to produce actinium-225 via spallation of a thorium-232 target. As part of previous R and D efforts carried out at Argonne National Laboratory recently in support of the proposed US FRIB facility, it was shown that a very effective production mechanism for actinium-225 is spallation of thorium-232 by high energy proton beams. The base-line simulation for the production rate of actinium-225 by this reaction mechanism is 8E12 atoms per second at 200 MeV proton beam energy with 50 g/cm2 thorium target and 100 kW beam power. An irradiation of one actinium-225 half-life (10 days) produces {approx}100 Ci of actinium-225. For a given beam current the reaction cross section increases slightly with energy to about 400 MeV and then decreases slightly for beam energies in the several GeV regime. The object of this effort is to refine the simulations at proton beam energies of 400 MeV and above up to about 8 GeV. Once completed, the simulations will be experimentally verified using 400 MeV and 8 Ge

  7. Comment: 223 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available 223.png Shinobu Okamoto (Kazusa DNA Research Institute ) licensed under CC Attribution2.1 Japan シアノバクテリア 電子顕微鏡写真 撮影:岡本忍(かずさDNA研究所) bando 2009/11/05 18:33:52 2010/02/16 10:25:27 ...

  8. 223Ra levels fed in the 223Fr β decay

    Science.gov (United States)

    Abdul-Hadi, A.; Barci, V.; Weiss, B.; Maria, H.; Ardisson, G.; Hussonnois, M.; Constantinescu, O.

    1993-01-01

    The 223Fr β decay was reinvestigated using high-resolution single γ spectrometry as well as γ-γ coincidence techniques. For single γ-spectra measurements, radiochemically pure 223Fr sources were obtained by chromatographic separation from a 75 MBq activity 227Ac parent source and continuously purified of 223Ra and daughters. The analysis of the γ spectra of 30 sources showed the existence of 131 γ lines, of which 87 are reported for the first time in the 223Fr β decay although many of them are observed following the 227Th α decay. The 223Fr half-life was remeasured and found to be T1/2=22.00+/-0.07 min. γ-γ-t coincidence measurements were also carried out with 223Fr purified sources. The 223Ra level scheme was built on the basis of our γ data, as well as 227Th α-decay data. Among the 32 excited 223Ra levels, of which 22 were also known from 227Th α decay, 13 are newly reported from 223Fr β decay. Low energy levels (E<400 keV) may be classified as parity doublet bands according to the predictions of the reflection asymmetric rotor model. Above a 700 keV gap, a coexistence of symmetric and asymmetric shapes including both static and dynamic octupole correlations is suggested.

  9. Analysis of the gamma spectra of the uranium, actinium, and thorium decay series

    Energy Technology Data Exchange (ETDEWEB)

    Momeni, M.H.

    1981-09-01

    This report describes the identification of radionuclides in the uranium, actinium, and thorium series by analysis of gamma spectra in the energy range of 40 to 1400 keV. Energies and absolute efficiencies for each gamma line were measured by means of a high-resolution germanium detector and compared with those in the literature. A gamma spectroscopy method, which utilizes an on-line computer for deconvolution of spectra, search and identification of each line, and estimation of activity for each radionuclide, was used to analyze soil and uranium tailings, and ore.

  10. Application of ion exchange and extraction chromatography to the separation of actinium from proton-irradiated thorium metal for analytical purposes.

    Science.gov (United States)

    Radchenko, V; Engle, J W; Wilson, J J; Maassen, J R; Nortier, F M; Taylor, W A; Birnbaum, E R; Hudston, L A; John, K D; Fassbender, M E

    2015-02-06

    Actinium-225 (t1/2=9.92d) is an α-emitting radionuclide with nuclear properties well-suited for use in targeted alpha therapy (TAT), a powerful treatment method for malignant tumors. Actinium-225 can also be utilized as a generator for (213)Bi (t1/2 45.6 min), which is another valuable candidate for TAT. Actinium-225 can be produced via proton irradiation of thorium metal; however, long-lived (227)Ac (t1/2=21.8a, 99% β(-), 1% α) is co-produced during this process and will impact the quality of the final product. Thus, accurate assays are needed to determine the (225)Ac/(227)Ac ratio, which is dependent on beam energy, irradiation time and target design. Accurate actinium assays, in turn, require efficient separation of actinium isotopes from both the Th matrix and highly radioactive activation by-products, especially radiolanthanides formed from proton-induced fission. In this study, we introduce a novel, selective chromatographic technique for the recovery and purification of actinium isotopes from irradiated Th matrices. A two-step sequence of cation exchange and extraction chromatography was implemented. Radiolanthanides were quantitatively removed from Ac, and no non-Ac radionuclidic impurities were detected in the final Ac fraction. An (225)Ac spike added prior to separation was recovered at ≥ 98%, and Ac decontamination from Th was found to be ≥ 10(6). The purified actinium fraction allowed for highly accurate (227)Ac determination at analytical scales, i.e., at (227)Ac activities of 1-100 kBq (27 nCi to 2.7 μCi).

  11. 36 CFR 223.130 - Scope.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Scope. 223.130 Section 223... NATIONAL FOREST SYSTEM TIMBER Suspension and Debarment of Timber Purchasers § 223.130 Scope. (a) This... causes and procedures for debarment and suspension and for determining the scope, duration, and...

  12. 32 CFR 223.5 - Responsibilities.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Responsibilities. 223.5 Section 223.5 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS DEPARTMENT OF DEFENSE UNCLASSIFIED CONTROLLED NUCLEAR INFORMATION (DOD UCNI) § 223.5 Responsibilities....

  13. 48 CFR 223.7101 - Procedures.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Procedures. 223.7101 Section 223.7101 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... Materials 223.7101 Procedures. (a) If the contracting officer is uncertain as to whether...

  14. 31 CFR 223.5 - Business.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Business. 223.5 Section 223.5 Money... OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE UNITED STATES § 223.5 Business. (a) The company must engage in the business of suretyship whether or not also...

  15. 33 CFR 136.223 - Compensation allowable.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Compensation allowable. 136.223 Section 136.223 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED... PROCEDURES; DESIGNATION OF SOURCE; AND ADVERTISEMENT Procedures for Particular Claims § 136.223...

  16. 50 CFR 223.205 - Sea turtles.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Sea turtles. 223.205 Section 223.205... Threatened Marine and Anadromous Species § 223.205 Sea turtles. (a) The prohibitions of section 9 of the Act (16 U.S.C. 1538) relating to endangered species apply to threatened species of sea turtle, except...

  17. 29 CFR 553.223 - Meal time.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Meal time. 553.223 Section 553.223 Labor Regulations... Enforcement Employees of Public Agencies Tour of Duty and Compensable Hours of Work Rules § 553.223 Meal time... personnel in accordance with section 7(a)(1) of the Act, the public agency may exclude meal time from...

  18. 50 CFR 22.3 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... as determined by the continuing absence of any adult, egg, or dependent young at the nest for at... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Definitions. 22.3 Section 22.3 Wildlife... (CONTINUED) EAGLE PERMITS Introduction § 22.3 Definitions. In addition to the definitions contained in...

  19. 32 CFR 223.4 - Policy.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Policy. 223.4 Section 223.4 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS DEPARTMENT OF DEFENSE UNCLASSIFIED CONTROLLED NUCLEAR INFORMATION (DOD UCNI) § 223.4 Policy. It is DoD policy: (a)...

  20. Thorium and actinium polyphosphonate compounds as bone-seeking alpha particle-emitting agents.

    Science.gov (United States)

    Henriksen, Gjermund; Bruland, Oyvind S; Larsen, Roy H

    2004-01-01

    The present study explores the use of alpha-particle-emitting, bone-seeking agents as candidates for targeted radiotherapy. Actinium and thorium 1,4,7,10 tetraazacyclododecane N,N',N'',N''' 1,4,7,10-tetra(methylene) phosphonic acid (DOTMP) and thorium-diethylene triamine N,N',N'' penta(methylene) phosphonic acid (DTMP) were prepared and their biodistribution evaluated in conventional Balb/C mice at four hours after injection. All three bone-seeking agents showed a high uptake in bone and a low uptake in soft tissues. Among the soft tissue organs, only kidney had a relatively high uptake. The femur/kidney ratios for 227Th-DTMP, 228-Ac-DOTMP and 227Th-DOTMP were 14.2, 7.6 and 6.0, respectively. A higher liver uptake of 228Ac-DOTMP was seen than for 227Th-DTMP and 227Th-DOTMP. This suggests that some demetallation of the 228Ac-DOTMP complex had occurred. The results indicate that 225Ac-DOTMP, 227Th-DOTMP and 227Th-DTMP have promising properties as potential therapeutic bone-seeking agents.

  1. Developments towards in-gas-jet laser spectroscopy studies of actinium isotopes at LISOL

    Energy Technology Data Exchange (ETDEWEB)

    Raeder, S., E-mail: s.raeder@gsi.de [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Helmholtz-Institut Mainz, 55128 Mainz (Germany); GSI Helmholtzzentrum für Schwerionenforschung GmbH, Planckstraße 1, 64291 Darmstadt (Germany); Bastin, B. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); Block, M. [Helmholtz-Institut Mainz, 55128 Mainz (Germany); GSI Helmholtzzentrum für Schwerionenforschung GmbH, Planckstraße 1, 64291 Darmstadt (Germany); Institut für Kernchemie, Johannes Gutenberg Universität, 55128 Mainz (Germany); Creemers, P. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Delahaye, P. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); Ferrer, R. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Fléchard, X. [LPC Caen, ENSICAEN, Université de Caen, CNRS/IN2P3, Caen (France); Franchoo, S. [Institute de Physique Nucléaire (IPN) d’Orsay, 91406 Orsay, Cedex (France); Ghys, L. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); SCK-CEN, Belgian Nuclear Research Center, Boeretang 200, 2400 Mol (Belgium); Gaffney, L.P.; Granados, C. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Heinke, R. [Institut für Physik, Johannes Gutenberg Universität, 55128 Mainz (Germany); Hijazi, L. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); and others

    2016-06-01

    To study exotic nuclides at the borders of stability with laser ionization and spectroscopy techniques, highest efficiencies in combination with a high spectral resolution are required. These usually opposing requirements are reconciled by applying the in-gas-laser ionization and spectroscopy (IGLIS) technique in the supersonic gas jet produced by a de Laval nozzle installed at the exit of the stopping gas cell. Carrying out laser ionization in the low-temperature and low density supersonic gas jet eliminates pressure broadening, which will significantly improve the spectral resolution. This article presents the required modifications at the Leuven Isotope Separator On-Line (LISOL) facility that are needed for the first on-line studies of in-gas-jet laser spectroscopy. Different geometries for the gas outlet and extraction ion guides have been tested for their performance regarding the acceptance of laser ionized species as well as for their differential pumping capacities. The specifications and performance of the temporarily installed high repetition rate laser system, including a narrow bandwidth injection-locked Ti:sapphire laser, are discussed and first preliminary results on neutron-deficient actinium isotopes are presented indicating the high capability of this novel technique.

  2. 36 CFR 223.88 - Bidding methods.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Bidding methods. 223.88 Section 223.88 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND... determines that departures are necessary to protect the public interest. (f) The Chief, Forest Service,...

  3. 36 CFR 223.279 - Personal use.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Personal use. 223.279 Section... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Forest Botanical Products § 223.279 Personal use. (a) Personal... for personal, non-commercial use up to the personal-use harvest level. (b) Personal use harvest...

  4. 27 CFR 46.223 - Tax credit.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Tax credit. 46.223 Section 46.223 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF... PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes...

  5. 36 CFR 223.141 - Suspension.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Suspension. 223.141 Section... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Suspension and Debarment of Timber Purchasers § 223.141 Suspension. (a) The suspending official may, in the public interest, suspend a purchaser on the basis...

  6. 36 CFR 223.227 - Sale advertisement.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Sale advertisement. 223.227... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Advertisement and Bids § 223.227 Sale advertisement. (a) The Forest Service shall advertise any special forest products sales with an appraised...

  7. 31 CFR 223.21 - Reinstatement.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Reinstatement. 223.21 Section 223.21 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  8. 31 CFR 223.20 - Final decisions.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Final decisions. 223.20 Section 223.20 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  9. 31 CFR 223.17 - Revocation.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Revocation. 223.17 Section 223.17 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  10. 31 CFR 223.4 - Deposits.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Deposits. 223.4 Section 223.4 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE UNITED...

  11. 31 CFR 223.8 - Financial reports.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Financial reports. 223.8 Section 223.8 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  12. 36 CFR 223.63 - Advertised rates.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Advertised rates. 223.63... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Appraisal and Pricing § 223.63 Advertised rates. Timber shall be advertised for sale at its appraised value. The road construction cost used...

  13. 50 CFR 223.208 - Corals.

    Science.gov (United States)

    2010-10-01

    ... 9(a)(1) of the ESA (16 U.S.C. 1538(a)(1)) relating to endangered species apply to elkhorn (Acropora palmata) and staghorn (A. cervicornis) corals listed as threatened in § 223.102(d), except as provided...

  14. Dosimetry of radium-223 and progeny

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, D.R. [Pacific Northwest National Lab., Richland, WA (United States); Sgouros, G. [Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    1999-01-01

    Radium-223 is a short-lived (11.4 d) alpha emitter with potential applications in radioimmunotherapy of cancer. Radium-223 can be complexed and linked to protein delivery molecules for specific tumor-cell targeting. It decays through a cascade of short-lived alpha- and beta-emitting daughters with emission of about 28 MeV of energy through complete decay. The first three alpha particles are essentially instantaneous. Photons associated with Ra-223 and progeny provide the means for tumor and normal-organ imaging and dosimetry. Two beta particles provide additional therapeutic value. Radium-223 may be produced economically and in sufficient amounts for widescale application. Many aspects of the chemistry of carrier-free isotope preparation, complexation, and linkage to the antibody have been developed and are being tested. The radiation dosimetry of a Ra-223-labeled antibody shows favorable tumor to normal tissue dose ratios for therapy. The 11.4-d half-life of Ra-223 allows sufficient time for immunoconjugate preparation, administration, and tumor localization by carrier antibodies before significant radiological decay takes place. If 0.01 percent of a 37 MBq (1 mCi) injection deposits in a one gram tumor mass, and if the activity is retained with a typical effective half-time (75 h), the absorbed dose will be 163 mGy MBq{sup {minus}1} (600 rad mCi{sup {minus}1}) administered. 49 refs., 5 figs., 2 tabs.

  15. Dicty_cDB: VHI223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VH (Link to library) VHI223 (Link to dictyBase) - - - Contig-U16260-1 VHI223P (Link to Original site) VHI...223F 570 VHI223Z 691 VHI223P 1241 - - Show VHI223 Library VH (Link to library) Clone ID VHI...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/VH/VHI2-A/VHI223Q.Seq.d/ Representative seq. ID VHI...223P (Link to Original site) Representative DNA sequence >VHI223 (VHI223Q) /CSM/VH/VHI2-A/VHI...ixikskknfkkk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value VHI

  16. 49 CFR 571.223 - Standard No. 223; Rear impact guards.

    Science.gov (United States)

    2010-10-01

    ... longitudinal vertical plane passing through the longitudinal centerline of the vehicle when the guard is... TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR VEHICLE SAFETY STANDARDS Federal Motor Vehicle Safety Standards § 571.223 Standard No. 223; Rear impact guards. S1. Scope. This...

  17. 7 CFR 1220.223 - Assessments.

    Science.gov (United States)

    2010-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Soybean Promotion and Research Order Expenses and Assessments § 1220.223 Assessments... net market price of the soybeans purchased. Each first purchaser shall remit such assessment to...

  18. 27 CFR 28.223 - Export marks.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Export marks. 28.223... Export marks. In addition to the marks and brands required to be placed on kegs, barrels, cases, crates... “Export” on each container or case before removal for export, for use on vessels or aircraft, or...

  19. 14 CFR 223.3 - Mandatory free transportation.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Mandatory free transportation. 223.3 Section 223.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS FREE AND REDUCED-RATE TRANSPORTATION General Provisions § 223.3...

  20. 24 CFR 884.223 - Leasing to eligible families.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Leasing to eligible families. 884.223 Section 884.223 Housing and Urban Development Regulations Relating to Housing and Urban...-ASIDE FOR SECTION 515 RURAL RENTAL HOUSING PROJECTS Project Development and Operation § 884.223...

  1. 48 CFR 1852.223-71 - Frequency authorization.

    Science.gov (United States)

    2010-10-01

    .... 1852.223-71 Section 1852.223-71 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... 1852.223-71 Frequency authorization. As prescribed in 1823.7101, insert the following clause: Frequency Authorization (DEC 1988) (a) Authorization of radio frequencies required in support of this contract shall...

  2. 47 CFR 1.223 - Petitions to intervene.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Petitions to intervene. 1.223 Section 1.223... and Issues § 1.223 Petitions to intervene. (a) Where, in cases involving applications for construction... accompanied by an affidavit of a qualified radio engineer which shall show, either by following the...

  3. 49 CFR 238.223 - Locomotive fuel tanks.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Locomotive fuel tanks. 238.223 Section 238.223... Equipment § 238.223 Locomotive fuel tanks. Locomotive fuel tanks shall comply with either the following or....21: (a) External fuel tanks. External locomotive fuel tanks shall comply with the...

  4. 14 CFR 417.223 - Flight hazard area analysis.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Flight hazard area analysis. 417.223 Section 417.223 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH SAFETY Flight Safety Analysis § 417.223 Flight hazard...

  5. Dicty_cDB: AHA223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ces producing significant alignments: (bits) Value AHA223 (AHA223Q) /CSM/AH/AHA2-A/AHA223Q.Seq.d/ 198 3e-50 ... Score E Sequences producing significant alignments: (bits) Value N AC115584 |AC115584.2 Dictyostelium disco...oducing significant alignments: (bits) Value S68963( S68963 ;S74306) phosphoprotein phosphatase (EC 3.1.3.16

  6. Production of actinium, thorium and radium isotopes from natural thorium irradiated with protons up to 141 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Ermolaev, S.V.; Zhuikov, B.L.; Kokhanyuk, V.M.; Matushko, V.L. [Russian Academy of Sciences, Moscow (Russian Federation). Inst. of Nuclear Research; Kalmykov, S.N. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Chemistry Dept.; Aliev, R.A. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Skobeltsyn Inst. of Nuclear Physics; Tananaev, I.G.; Myasoedov, B.F. [Russian Academy of Sciences, Moscow (Russian Federation). A.N. Frumkin Inst. of Physical Chemistry and Electrochemistry

    2012-07-01

    Cross sections of {sup 225}Ac, {sup 227}Ac, {sup 227}Th and {sup 228}Th in thorium-232 targets irradiated with protons in the energy range 21-141 MeV have been measured. Based on these data, production yields of {sup 225}Ac and {sup 223}Ra in thick thorium targets have been calculated. It is possible to produce in proton energy range 60-140 MeV about 96 GBq (2.6 Ci) {sup 225}Ac per 10-d irradiation with 100 {mu}A proton beam current and 10-d decay, and much higher amount of {sup 223}Ra. The impurities of {sup 227}Ac and {sup 224}Ra are important and need to be assessed for further medical applications. (orig.)

  7. 36 CFR 223.229 - Contents of prospectus.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Contents of prospectus. 223.229 Section 223.229 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Advertisement and Bids §...

  8. 36 CFR 223.87 - Requirements of bidders concerning exports.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Requirements of bidders concerning exports. 223.87 Section 223.87 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and...

  9. 36 CFR 223.89 - Relation to other bidders.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Relation to other bidders. 223.89 Section 223.89 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and Bids §...

  10. 36 CFR 223.83 - Contents of prospectus.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Contents of prospectus. 223.83 Section 223.83 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and Bids §...

  11. 36 CFR 223.85 - Noncompetitive sale of timber.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Noncompetitive sale of timber. 223.85 Section 223.85 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE... further advertisement, at not less than appraised value, any timber previously advertised for...

  12. 48 CFR 352.223-70 - Safety and health.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Safety and health. 352.223-70 Section 352.223-70 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES CLAUSES AND... health. As prescribed in 323.7002, the Contracting Officer shall insert the following clause: Safety...

  13. 48 CFR 1852.223-70 - Safety and health.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Safety and health. 1852.223... 1852.223-70 Safety and health. As prescribed in 1823.7004(c), insert the following clause: Safety and Health (APR 2002) (a) Safety is the freedom from those conditions that can cause death,...

  14. 46 CFR 502.223 - Decisions-Administrative law judges.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Decisions-Administrative law judges. 502.223 Section 502... PROCEDURE Briefs; Requests for Findings; Decisions; Exceptions § 502.223 Decisions—Administrative law judges. To the administrative law judges is delegated the authority to make and serve initial or...

  15. 36 CFR 223.277 - Forest botanical products definition.

    Science.gov (United States)

    2010-07-01

    ... botanical products definition. As used in this subpart, the following term shall mean: Forest botanical... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Forest botanical products definition. 223.277 Section 223.277 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT...

  16. 36 CFR 223.216 - Special Forest Products definitions.

    Science.gov (United States)

    2010-07-01

    ... Forest Products definitions. As used in this subpart: Person: Any individual, partnership, corporation... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Special Forest Products definitions. 223.216 Section 223.216 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT...

  17. 31 CFR 223.3 - Issuance of certificates of authority.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Issuance of certificates of authority. 223.3 Section 223.3 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... United States or of any State, or it is limited by its articles of incorporation or corporate charter...

  18. 42 CFR 93.223 - Research misconduct proceeding.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Research misconduct proceeding. 93.223 Section 93.223 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES HEALTH ASSESSMENTS AND HEALTH EFFECTS STUDIES OF HAZARDOUS SUBSTANCES RELEASES AND FACILITIES PUBLIC HEALTH...

  19. 31 CFR 223.12 - Recognition as reinsurer.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Recognition as reinsurer. 223.12 Section 223.12 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  20. 31 CFR 223.10 - Limitation of risk.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Limitation of risk. 223.10 Section 223.10 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  1. 31 CFR 223.11 - Limitation of risk: Protective methods.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Limitation of risk: Protective methods. 223.11 Section 223.11 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES...

  2. 31 CFR 223.6 - Requirements applicable to surety companies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Requirements applicable to surety companies. 223.6 Section 223.6 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES...

  3. 31 CFR 223.2 - Application for certificate of authority.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Application for certificate of authority. 223.2 Section 223.2 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES...

  4. 31 CFR 223.14 - Schedules of single risks.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Schedules of single risks. 223.14 Section 223.14 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  5. 31 CFR 223.19 - Informal hearing on agency complaints.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Informal hearing on agency complaints. 223.19 Section 223.19 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING...

  6. 31 CFR 223.9 - Valuation of assets and liabilities.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Valuation of assets and liabilities. 223.9 Section 223.9 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING...

  7. 31 CFR 223.18 - Performance of agency obligations.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Performance of agency obligations. 223.18 Section 223.18 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING...

  8. 31 CFR 223.7 - Investment of capital and assets.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Investment of capital and assets. 223.7 Section 223.7 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING...

  9. 31 CFR 223.16 - List of certificate holding companies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false List of certificate holding companies. 223.16 Section 223.16 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING...

  10. 31 CFR 223.1 - Certificate of authority.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Certificate of authority. 223.1 Section 223.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  11. 22 CFR 22.3 - Remittances in the United States.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Remittances in the United States. 22.3 Section...-DEPARTMENT OF STATE AND FOREIGN SERVICE § 22.3 Remittances in the United States. (a) Type of remittance. Remittances shall be in the form of: (1) Check or bank draft drawn on a bank in the United States; (2)...

  12. 24 CFR 248.223 - Alternative State strategy.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Alternative State strategy. 248.223... Preservation Act of 1987 § 248.223 Alternative State strategy. (a) The Commissioner may approve a State strategy providing for State approval of plans of action that involve termination of low...

  13. 29 CFR 1952.223 - Compliance staffing benchmarks.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Compliance staffing benchmarks. 1952.223 Section 1952.223... Compliance staffing benchmarks. Under the terms of the 1978 Court Order in AFL-CIO v. Marshall compliance staffing levels (benchmarks) necessary for a “fully effective” enforcement program were required to...

  14. Level Scheme of {sup 223}Fr; Estudio del esquema de niveles del {sup 223}Fr

    Energy Technology Data Exchange (ETDEWEB)

    Gaeta, R.; Gonzalez, L.; Roldan, C.

    1972-07-01

    A study has been made of the decay of {sup 227}Ac at levels of {sub 223}Fr, means of alpha Spectrometers of Si barrier detector and gamma Spectrometers of Ge(Li). The rotational bands 1/2-(541 {down_arrow}), 1/2-(530 {up_arrow}) and 3/2-(532 {down_arrow}) have been identified, as well as two octupolar bands associated with the fundamental one. The results obtained indicate that the unified model is applicable in this intermediate zone of the nuclide chart. (Author) 150 refs.

  15. 29 CFR 500.223 - Caption of proceeding.

    Science.gov (United States)

    2010-07-01

    ... SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Rules of Practice § 500.223 Caption of...” shall be identified as plaintiff and the person requesting such hearing shall be named as...

  16. Genetics Home Reference: 9q22.3 microdeletion

    Science.gov (United States)

    ... the characteristic features of a genetic condition called Gorlin syndrome . Many individuals with a 9q22.3 microdeletion ... 3 microdeletions also cause the characteristic features of Gorlin syndrome (also known as nevoid basal cell carcinoma ...

  17. MicroRNA-223 is neuroprotective by targeting glutamate receptors

    Science.gov (United States)

    Harraz, Maged M.; Eacker, Stephen M.; Wang, Xueqing; Dawson, Ted M.; Dawson, Valina L.

    2012-01-01

    Stroke is a major cause of mortality and morbidity worldwide. Extracellular glutamate accumulation leading to overstimulation of the ionotropic glutamate receptors mediates neuronal injury in stroke and in neurodegenerative disorders. Here we show that miR-223 controls the response to neuronal injury by regulating the functional expression of the glutamate receptor subunits GluR2 and NR2B in brain. Overexpression of miR-223 lowers the levels of GluR2 and NR2B by targeting 3′-UTR target sites (TSs) in GluR2 and NR2B, inhibits NMDA-induced calcium influx in hippocampal neurons, and protects the brain from neuronal cell death following transient global ischemia and excitotoxic injury. MiR-223 deficiency results in higher levels of NR2B and GluR2, enhanced NMDA-induced calcium influx, and increased miniature excitatory postsynaptic currents in hippocampal neurons. In addition, the absence of MiR-223 leads to contextual, but not cued memory deficits and increased neuronal cell death following transient global ischemia and excitotoxicity. These data identify miR-223 as a major regulator of the expression of GluR2 and NR2B, and suggest a therapeutic role for miR-223 in stroke and other excitotoxic neuronal disorders. PMID:23112146

  18. miR-223 reverses experimental pulmonary arterial hypertension.

    Science.gov (United States)

    Meloche, Jolyane; Le Guen, Marie; Potus, François; Vinck, Jérôme; Ranchoux, Benoit; Johnson, Ian; Antigny, Fabrice; Tremblay, Eve; Breuils-Bonnet, Sandra; Perros, Frederic; Provencher, Steeve; Bonnet, Sébastien

    2015-09-15

    Pulmonary arterial hypertension (PAH) is a devastating disease affecting lung vasculature. The pulmonary arteries become occluded due to increased proliferation and suppressed apoptosis of the pulmonary artery smooth muscle cells (PASMCs) within the vascular wall. It was recently shown that DNA damage could trigger this phenotype by upregulating poly(ADP-ribose)polymerase 1 (PARP-1) expression, although the exact mechanism remains unclear. In silico analyses and studies in cancer demonstrated that microRNA miR-223 targets PARP-1. We thus hypothesized that miR-223 downregulation triggers PARP-1 overexpression, as well as the proliferation/apoptosis imbalance observed in PAH. We provide evidence that miR-223 is downregulated in human PAH lungs, distal PAs, and isolated PASMCs. Furthermore, using a gain and loss of function approach, we showed that increased hypoxia-inducible factor 1α, which is observed in PAH, triggers this decrease in miR-223 expression and subsequent overexpression of PARP-1 allowing PAH-PASMC proliferation and resistance to apoptosis. Finally, we demonstrated that restoring the expression of miR-223 in lungs of rats with monocrotaline-induced PAH reversed established PAH and provided beneficial effects on vascular remodeling, pulmonary resistance, right ventricle hypertrophy, and survival. We provide evidence that miR-223 downregulation in PAH plays an important role in numerous pathways implicated in the disease and restoring its expression is able to reverse PAH.

  19. Optical imaging of L723: the structure of HH 223

    CERN Document Server

    López, R; Gómez, G; Riera, A

    2006-01-01

    We imaged the Lynds 723 dark nebula (L723) with the aim of studying the morphology of the Herbig-Haro object HH 223 and other line-emission nebula detected in the region. We obtained deep narrow-band images in the Halpha and [SII] lines and in the continuum nearby Halpha of a field of ~5' of the L723 dark nebula centered on HH 223. The Halpha and [SII] images reveal the detailed morphology of HH 223, unresolved in previous optical images. Both images show a quite complex knotty, wiggling structure embedded in a low-emission nebula. Comparison between the [SII] and Halpha fluxes of the knots are indicative of variations in the excitation conditions through HH 223. In addition, several other faint nebula are detected in Halpha a few arcmin to the SE and to the NW of HH 223, all of them lying projected onto the east-west pair of lobes of the quadrupolar CO outflow. Comparison between the Halpha and the continuum images confirms the HH-like nature of the Vrba object V83, while the Vrba objects V84 and V85 are ide...

  20. Recovery of Ra-223 from natural thorium irradiated by protons

    Energy Technology Data Exchange (ETDEWEB)

    Vasiliev, Aleksandr N.; Ostapenko, Valentina S. [Lomonosov Moscow State Univ. (Russian Federation); Russian Academy of Sciences, Moscow-Troitsk (Russian Federation). Inst. for Nuclear Research; Lapshina, Elena V.; Ermolaev, Stanislav V.; Zhuikov, Boris L. [Russian Academy of Sciences, Moscow-Troitsk (Russian Federation). Inst. for Nuclear Research; Danilov, Sergey S. [Lomonosov Moscow State Univ. (Russian Federation); Kalmykov, Stepan N. [Lomonosov Moscow State Univ. (Russian Federation); National Research Center ' Kurchatov Institute' (NRC ' Kurchatov Institute' ), Moscow (Russian Federation)

    2016-11-01

    Irradiation of natural thorium with medium-energy protons is considered to be a prospective approach to large-scale production of {sup 225}Ac and {sup 223}Ra. In addition to the earlier-developed method of {sup 225}Ac isolation, the present work focuses on the simultaneous recovery of {sup 223}Ra from the same thorium target. Radiochemical procedure is based on liquid-liquid extraction, cation exchange and extraction chromatography. The procedure provides separation of radium from spallation and fission products generated in the thorium target. High chemical yield (85-90%) and radionuclide purity of {sup 223}Ra (> 99.8% except {sup 224}Ra and {sup 225}Ra isotopes) have been achieved.

  1. The Multiple Roles of Microrna-223 in Regulating Bone Metabolism

    Directory of Open Access Journals (Sweden)

    Yong Xie

    2015-10-01

    Full Text Available Bone metabolism is a lifelong process for maintaining skeletal system homeostasis, which is regulated by bone-resorbing osteoclasts and bone-forming osteoblasts. Aberrant differentiation of osteoclasts and osteoblasts leads to imbalanced bone metabolism, resulting in ossification and osteolysis diseases. MicroRNAs (miRNAs are pivotal factors in regulating bone metabolism via post-transcriptional inhibition of target genes. Recent studies have revealed that miR-223 exerts multiple effects on bone metabolism, especially in the processes of osteoclast and osteoblasts differentiation. In this review, we highlight the roles of miR-223 during the processes of osteoclast and osteoblast differentiation, as well as the potential clinical applications of miR-223 in bone metabolism disorders.

  2. 48 CFR 52.223-7 - Notice of radioactive materials.

    Science.gov (United States)

    2010-10-01

    ....223-7 Notice of radioactive materials. As prescribed in 23.602, insert the following clause: Notice of Radioactive Materials (JAN 1997) (a) The Contractor shall notify the Contracting Officer or designee, in... contract of, items containing either (1) radioactive material requiring specific licensing under...

  3. 36 CFR 223.143 - Procedures for suspension.

    Science.gov (United States)

    2010-07-01

    ... SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Suspension and Debarment of Timber Purchasers § 223... official finds that there exists a genuine dispute over facts material to the suspension, respondent(s... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Procedures for...

  4. 36 CFR 223.139 - Period of debarment.

    Science.gov (United States)

    2010-07-01

    ... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Suspension and Debarment of Timber Purchasers § 223.139 Period of... debarring official shall consider any suspension period or period since issuance of the notice of proposed...) Newly discovered material evidence; (2) Reversal of the conviction or judgment upon which the...

  5. 36 CFR 223.142 - Causes for suspension.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Causes for suspension. 223... Causes for suspension. (a) The suspending official may suspend a purchaser suspected, upon adequate... responsibility of a purchase of Government timber. (2) Indictment for any of the causes listed in paragraph...

  6. THE METABOLSIM AND TOXICITY OF RADIUM-223 IN RATS

    Energy Technology Data Exchange (ETDEWEB)

    Durbin, Patricia; Durbin, Patricia W.; Asling, C. Willet.; Jeung, Nylan; Williams, Marilyn H.; Post, James.; Johnston, Muriel E.; Hamilton, Joseph G.

    1958-02-21

    This report covers studies of the excretion and retention of 'tracer' and toxic doses of the 11.2-day Ra{sup 223} isotope, its acute toxicity (organ weight changes, gross and microscopic pathology, and Fe{sup 59} utilization by the bone marrow), and long-term histopathological changes and alterations in the hemogram.

  7. Radium-223 in metastatic castration resistant prostate cancer

    Institute of Scientific and Technical Information of China (English)

    Winston Vuong; Oliver Sartor; Sumanta K Pal

    2014-01-01

    In 2004, docetaxel was approved for the treatment of metastatic castration-resistant prostate cancer (mCRPC). For the next several years, there was a lull in drug approvals. However, from 2010 onwards, 5 additional therapies have been approved on the basis of showing a survival beneift in phase III studies. These agents include sipuleucel-T, cabazitaxel, abiraterone, enzalutamide and (most recently) radium-223. Amongst radiopharmaceuticals currently used for advanced prostate cancer (e.g. samarium-153 and strontium-89), radium-223 possesses several unique properties. As an alpha-emitting compound, the agent produces a high-energy output over a short range, facilitating selective destruction of tissue within the bone in the region of osteoblastic lesions while sparing surrounding normal tissue. The current review will outline biological rationale for radium-223 and also provide an overview of preclinical and clinical development of the agent. Rational sequencing of radium-223 and combinations, in the increasingly complex landscape of mCRPC will be discussed, along with factors inlfuencing clinical implementation.

  8. Radium-223 in metastatic castration resistant prostate cancer

    Directory of Open Access Journals (Sweden)

    Winston Vuong

    2014-06-01

    Full Text Available In 2004, docetaxel was approved for the treatment of metastatic castration-resistant prostate cancer (mCRPC. For the next several years, there was a lull in drug approvals. However, from 2010 onwards, 5 additional therapies have been approved on the basis of showing a survival benefit in phase III studies. These agents include sipuleucel-T, cabazitaxel, abiraterone, enzalutamide and (most recently radium-223. Amongst radiopharmaceuticals currently used for advanced prostate cancer (e.g. samarium-153 and strontium-89, radium-223 possesses several unique properties. As an alpha-emitting compound, the agent produces a high-energy output over a short range, facilitating selective destruction of tissue within the bone in the region of osteoblastic lesions while sparing surrounding normal tissue. The current review will outline biological rationale for radium-223 and also provide an overview of preclinical and clinical development of the agent. Rational sequencing of radium-223 and combinations, in the increasingly complex landscape of mCRPC will be discussed, along with factors influencing clinical implementation.

  9. 36 CFR 223.228 - Contents of advertisement.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Contents of advertisement... SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Advertisement and Bids § 223.228 Contents of advertisement. The Forest Service shall include the following information in...

  10. 36 CFR 223.80 - When advertisement is required.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false When advertisement is... AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and Bids § 223.80 When advertisement is required. Except as otherwise provided in this part each sale in...

  11. 36 CFR 223.82 - Contents of advertisement.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Contents of advertisement... SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and Bids § 223.82 Contents of advertisement. (a) A timber sale advertisement shall include the following...

  12. 36 CFR 223.81 - Shorter advertising periods in emergencies.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Shorter advertising periods... OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and Bids § 223.81 Shorter advertising periods in emergencies. In emergency situations where...

  13. 36 CFR 223.218 - Consistency with plans, environmental standards, and other management requirements.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Consistency with plans, environmental standards, and other management requirements. 223.218 Section 223.218 Parks, Forests, and Public... Special Forest Products § 223.218 Consistency with plans, environmental standards, and other...

  14. 36 CFR 223.30 - Consistency with plans, environmental standards, and other management requirements.

    Science.gov (United States)

    2010-07-01

    ..., environmental standards, and other management requirements. 223.30 Section 223.30 Parks, Forests, and Public... Timber Sale Contracts Contract Conditions and Provisions § 223.30 Consistency with plans, environmental standards, and other management requirements. The approving officer will insure that each timber...

  15. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions...

  16. 36 CFR 223.10 - Free use to Alaskan settlers, miners, residents, and prospectors.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Free use to Alaskan settlers, miners, residents, and prospectors. 223.10 Section 223.10 Parks, Forests, and Public Property FOREST... § 223.10 Free use to Alaskan settlers, miners, residents, and prospectors. Bona fide settlers,...

  17. 36 CFR 223.192 - Procedures for a non-manufacturer.

    Science.gov (United States)

    2010-07-01

    ...-manufacturer. 223.192 Section 223.192 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... Relief Act of 1990 Program § 223.192 Procedures for a non-manufacturer. (a) Persons who do not own or operate a manufacturing facility (non-manufacturer) are not eligible to apply for or be granted a...

  18. 47 CFR 80.223 - Special requirements for survival craft stations.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Special requirements for survival craft stations. 80.223 Section 80.223 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES General Technical Standards § 80.223...

  19. 47 CFR 15.223 - Operation in the band 1.705-10 MHz.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Operation in the band 1.705-10 MHz. 15.223 Section 15.223 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY DEVICES Intentional Radiators Radiated Emission Limits, Additional Provisions § 15.223 Operation in the band...

  20. 37 CFR 2.23 - Additional requirements for TEAS Plus application.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Additional requirements for TEAS Plus application. 2.23 Section 2.23 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND... § 2.23 Additional requirements for TEAS Plus application. (a) In addition to the filing...

  1. 36 CFR 223.12 - Permission to cut, damage, or destroy trees without advertisement.

    Science.gov (United States)

    2010-07-01

    ... destroy trees without advertisement. 223.12 Section 223.12 Parks, Forests, and Public Property FOREST... § 223.12 Permission to cut, damage, or destroy trees without advertisement. Permission may be granted to... lands without advertisement when necessary for the occupancy of a right-of-way or other authorized...

  2. 48 CFR 52.223-18 - Contractor Policy to Ban Text Messaging While Driving.

    Science.gov (United States)

    2010-10-01

    ... Text Messaging While Driving. 52.223-18 Section 52.223-18 Federal Acquisition Regulations System... Text of Provisions and Clauses 52.223-18 Contractor Policy to Ban Text Messaging While Driving. As prescribed in 23.1105, insert the following clause: CONTRACTOR POLICY TO BAN TEXT MESSAGING WHILE...

  3. 48 CFR 52.223-2 - Affirmative Procurement of Biobased Products Under Service and Construction Contracts.

    Science.gov (United States)

    2010-10-01

    ... Biobased Products Under Service and Construction Contracts. 52.223-2 Section 52.223-2 Federal Acquisition... Procurement of Biobased Products Under Service and Construction Contracts (DEC 2007) (a) In the performance of... CONTRACT CLAUSES Text of Provisions and Clauses 52.223-2 Affirmative Procurement of Biobased Products...

  4. 14 CFR 120.223 - Alcohol misuse information, training, and substance abuse professionals.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Alcohol misuse information, training, and substance abuse professionals. 120.223 Section 120.223 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS DRUG AND ALCOHOL TESTING PROGRAM Alcohol Testing Program Requirements § 120.223...

  5. 36 CFR 223.281 - Monitoring and revising sustainable harvest levels.

    Science.gov (United States)

    2010-07-01

    ... sustainable harvest levels. 223.281 Section 223.281 Parks, Forests, and Public Property FOREST SERVICE....281 Monitoring and revising sustainable harvest levels. The Forest Service shall monitor and revise sustainable harvest levels for forest botanical products in accordance with § 223.219 of subpart G....

  6. 42 CFR 431.223 - Denial or dismissal of request for a hearing.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Denial or dismissal of request for a hearing. 431.223 Section 431.223 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND... Fair Hearings for Applicants and Recipients Right to Hearing § 431.223 Denial or dismissal of...

  7. Radiation exposure of relatives of patients treated with Ra-223 dichloride; Strahlenexposition von Angehoerigen bei Therapie mit Ra-223-Dichlorid

    Energy Technology Data Exchange (ETDEWEB)

    Wanke, C.; Szermerski, B.; Solle, A.; Geworski, L. [Medizinische Hochschule Hannover (Germany). Stabsstelle Strahlenschutz und Abt. Medizinische Physik; Pinkert, J. [Bayer Vital GmbH, Leverkusen (Germany); Kranert, W.T. [Frankfurt Univ. (Germany). Klinik fuer Nuklearmedizin; Andreeff, M. [Universitaetsklinikum ' ' Carl Gustav Carus' ' TU Dresden (Germany). Klinik fuer Nuklearmedizin

    2015-07-01

    Since November 2013, a radiopharmaceutical containing Ra-223 dichloride as active substance is approved in the European Union for patients with castration resistant prostate cancer with symptomatic bone metastases and no known visceral metastases. Ra-223 (T{sub 1/2} = 11.43 d) decays via a chain of 4 alpha and 2 beta decays. This therapy is presently the only application of an alpha emitter in clinical routine therapy. To show that the exposure of relatives and caregivers of patients treated with Ra-223 dichloride in an outpatient setting does not exceed a value of 1 mSv, the multicenter study ''RAPSODY'' was conducted. As Ra-223 and most of its progeny emit alpha particles, the internal exposure had to be evaluated in particular. Within this study, measurements of the radiation emitted from the patient were performed using standard dose-rate meters. Wipe-tests were taken in the patients' homes to identify significant contaminations and evaluated by liquid scintillation counting. Samples of saliva and sweat were taken and measured using gamma spectrometry. Ra-223 disintegrates to the noble gas Rn-219 and was measured in the exhaled breath from the patients using conventional Radon Monitors. Furthermore, a computational fluid dynamics simulation (CFD) was performed to assess the radioactivity in the air, which could be inhaled by persons close to the patient. Conclusions: The potential exposure of relatives and caregivers by external irradiation and incorporation of radioactivity exhaled or excreted by the patient with saliva or sweat is well below 1 mSv. No objections are seen regarding outpatient treatment. This paper summarizes contents of a poster presented at the Annual Meeting of the Society of Nuclear Medicine and Molecular Imaging.

  8. The miR-223 host non-coding transcript linc-223 induces IRF4 expression in acute myeloid leukemia by acting as a competing endogenous RNA

    KAUST Repository

    Mangiavacchi, Arianna

    2016-08-10

    Alterations in genetic programs required for terminal myeloid differentiation and aberrant proliferation characterize acute myeloid leukemia (AML) cells. Here, we identify the host transcript of miR-223, linc-223, as a novel functional long non-coding RNA (lncRNA) in AML. We show that from the primary nuclear transcript, the alternative production of miR-223 and linc-223 is finely regulated during monocytic differentiation. Moreover, linc-223 expression inhibits cell cycle progression and promotes monocytic differentiation of AML cells. We also demonstrate that endogenous linc-223 localizes in the cytoplasm and acts as a competing endogenous RNA for miR-125-5p, an oncogenic microRNA in leukemia. In particular, we show that linc-223 directly binds to miR-125-5p and that its knockdown increases the repressing activity of miR-125-5p resulting in the downregulation of its target interferon regulatory factor 4 (IRF4), which it was previously shown to inhibit the oncogenic activity of miR-125-5p in vivo. Furthermore, data from primary AML samples show significant downregulation of linc-223 in different AML subtypes. Therein, these findings indicate that the newly identified lncRNA linc-223 may have an important role in myeloid differentiation and leukemogenesis, at least in part, by cross-talking with IRF4 mRNA.

  9. The Dark Matter filament between Abell 222/223

    Science.gov (United States)

    Dietrich, Jörg P.; Werner, Norbert; Clowe, Douglas; Finoguenov, Alexis; Kitching, Tom; Miller, Lance; Simionescu, Aurora

    2016-10-01

    Weak lensing detections and measurements of filaments have been elusive for a long time. The reason is that the low density contrast of filaments generally pushes the weak lensing signal to unobservably low scales. To nevertheless map the dark matter in filaments exquisite data and unusual systems are necessary. SuprimeCam observations of the supercluster system Abell 222/223 provided the required combination of excellent seeing images and a fortuitous alignment of the filament with the line-of-sight. This boosted the lensing signal to a detectable level and led to the first weak lensing mass measurement of a large-scale structure filament. The filament connecting Abell 222 and Abell 223 is now the only one traced by the galaxy distribution, dark matter, and X-ray emission from the hottest phase of the warm-hot intergalactic medium. The combination of these data allows us to put the first constraints on the hot gas fraction in filaments.

  10. Schizophrenia susceptibility gene locus at Xp22.3.

    Science.gov (United States)

    Milunsky, J; Huang, X L; Wyandt, H E; Milunsky, A

    1999-06-01

    Multiple genetic loci have been implicated in the search for schizophrenia susceptibility genes, none having been proven as causal. Genetic heterogeneity is probable in the polygenic etiology of schizophrenia. We report on two unrelated Caucasian women with paranoid schizophrenia (meeting Diagnostic and Statistical Manual of Mental Disorders (DSM IV) criteria) who have an Xp22.3 overlapping deletion characterized by fluorescence in situ hybridization (FISH). Patient 1 was previously reported by us (Wyandt HE, Bugeau-Michaud L, Skare JC, Milunsky A. Partial duplication of Xp: a case report and review of previously reported cases. Amer J Med Genet 1991: 40: 280-283) to have a de novo partial duplication of Xp. At that time, she was a 24-year-old woman with short stature, irregular menses, other abnormalities suggestive of Turner syndrome, and paranoid schizophrenia. Recently, FISH analysis demonstrated that she has an inverted duplication (X)(p22.1p11.2) and a microscopic deletion (X)(p22.2p22.3) between DXS1233 and DXS7108 spanning approximately 16-18 cM. Patient 2 is a 14-year-old girl with short stature, learning disabilities, and paranoid schizophrenia. High-resolution chromosome analysis revealed a de novo deletion involving Xp22. FISH analysis showed that the deletion (X)(p22.2p22.3) spanned 10-12 cM between AFMB290XG5 and DXS1060. Given that deletions of Xp22 are not common events, the occurrence of two unrelated schizophrenia patients with an overlapping deletion of this region would be extraordinarily rare. Hence, the deletion within Xp22.3 almost certainly contains a gene involved in the pathogenesis of paranoid schizophrenia.

  11. MOS Mapping of the NIR Outflow HH 223

    Science.gov (United States)

    López, R.; Acosta-Pulido, J. A.; Estalella, R.; Gómez, G.; García-Lorenzo, B.

    2016-10-01

    The Multi-Object-Spectroscopy (MOS) mode of LIRIS was used to map the near-IR stellar outflow HH 223, in the dark cloud Lynds 723 (L723). HH 223 spatially coincides with the east-west component of the L723 quadrupolar CO outflow. The radio continuum source SMA2, towards the center of the quadrupolar CO outflow, hides the YSO that seems to power both the near-IR and the CO outflows. To map the S-shaped, near-IR emission of HH 223, extending ˜ 5', an appropriate mask was designed, with 16 rectangular slitlets. J, H and K-band spectra (R ˜eq 2500) were obtained through the mask. The kinematics of the neutral (H2) and ionized ([FeII]) gas outflow was derived from these data. The results confirm that both the near-IR and the CO outflows have a common driving source. To our knowledge, this is the first use of the MOS-LIRIS observing mode with the mask designed ad hoc to fit several extended, nonaligned targets.

  12. A New Analysis in the Field of the Open Cluster Collinder 223

    Institute of Scientific and Technical Information of China (English)

    A. L. Tadross

    2004-01-01

    The present study of the open cluster Collinder 223 (Cr 223) depended greatly on the photoelectric data of Claria and Lapasset. We use the data in conjunction with the AAO/DSS1 image of the cluster in a re-investigation to improve the main parameters of Cr 223, including the stellar density, the position of the cluster's center, the cluster's diameter. Its luminosity function, mass function, and total mass are also estimated.

  13. Glut4 Palmitoylation at Cys223 Plays a Critical Role in Glut4 Membrane Trafficking

    OpenAIRE

    Ren, Wenying; Sun, Yingmin; Du, Keyong

    2015-01-01

    Recently, we identified Glut4 as a palmitoylated protein in adipocytes. To understand the role of Glut4 palmitoylation in Glut4 membrane trafficking, a process that is essential for maintenance of whole body glucose homeostasis, we have characterized Glut4 palmitoylation. We found that Glut4 is palmitoylated at Cys223 and Glut4 palmitoylation at Cys223 is essential for insulin dependent Glut4 membrane translocation as substitution of Cys223 with a serine residue in Glut4 (C223S Glut4) diminis...

  14. 36 CFR 223.232 - Disclosure of relation to other bidders.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Disclosure of relation to other bidders. 223.232 Section 223.232 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Advertisement and...

  15. 36 CFR 223.84 - Small business bid form provisions on sales with specified road construction.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Small business bid form provisions on sales with specified road construction. 223.84 Section 223.84 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM...

  16. 36 CFR 223.86 - Bid restriction on resale of noncompleted contract.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Bid restriction on resale of noncompleted contract. 223.86 Section 223.86 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and...

  17. 36 CFR 223.230 - Bid restriction on resale of incomplete contracts, permits, or other instruments.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Bid restriction on resale of incomplete contracts, permits, or other instruments. 223.230 Section 223.230 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM...

  18. 48 CFR 952.223 - Clauses related to environment, energy and water efficiency, renewable energy technologies...

    Science.gov (United States)

    2010-10-01

    ... environment, energy and water efficiency, renewable energy technologies, occupational safety, and drug-free workplace. 952.223 Section 952.223 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CLAUSES AND... related to environment, energy and water efficiency, renewable energy technologies, occupational...

  19. 48 CFR 1552.223-71 - EPA Green Meetings and Conferences.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true EPA Green Meetings and Conferences. 1552.223-71 Section 1552.223-71 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION... provision or language substantially the same as the provision in solicitations for meetings and...

  20. 36 CFR 223.217 - Authority to dispose of special forest products.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Authority to dispose of special forest products. 223.217 Section 223.217 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products §...

  1. 36 CFR 223.241 - Disposal of seized special forest products.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Disposal of seized special forest products. 223.241 Section 223.241 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Award...

  2. Comment on ``Interpretation of the fine structure in the 14C radioactive decay of 223'

    Science.gov (United States)

    Hussonnois, M.; Le Du, J. F.; Brillard, L.; Ardisson, G.

    1991-12-01

    Priority of our interpretation of the fine structure in the 14C radioactive decay of 223Ra is asserted. It seems that the deformation parameter values, used in the framework of ARM to interpret properties of both 223Ra ground and excited states, partly allow for the qualitative interpretation of the experimental hindrance factors to the 209Pb states.

  3. 36 CFR 223.280 - Waiver of fees and/or fair market value.

    Science.gov (United States)

    2010-07-01

    ... harvest forest botanical products for cultural, ceremonial, and/or traditional purposes. Such purposes... harvest forest botanical products for cultural, ceremonial, and/or traditional purposes in accordance with... market value. 223.280 Section 223.280 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT...

  4. 36 CFR 223.278 - Sale of forest botanical products and collection of fees.

    Science.gov (United States)

    2010-07-01

    ... the costs of any environmental or other analysis. The fair market value of forest botanical products... botanical product's fair market value. All other aspects related to the sale of forest botanical products... products and collection of fees. 223.278 Section 223.278 Parks, Forests, and Public Property FOREST...

  5. Radium-223 behandling af knoglemetastaser ved kastrationsrefraktær prostatacancer

    DEFF Research Database (Denmark)

    Mortensen, Jann; Højgaard, Liselotte

    2014-01-01

    The alpha emitter Radium-223 ((22)3Ra-Cl2) is a bone-seeking radionuclide studied as a new treatment for patients with bone metastases from hormone refractory prostate cancer. More than 1,000 patients have been included in clinical phase I-III tests showing significant reduction in alkaline...

  6. Radium-223 treatment of bone metastases from castration-resistant prostate cancer

    DEFF Research Database (Denmark)

    Mortensen, Jann; Højgaard, Liselotte

    2014-01-01

    The alpha emitter Radium-223 ((22)3Ra-Cl2) is a bone-seeking radionuclide studied as a new treatment for patients with bone metastases from hormone refractory prostate cancer. More than 1,000 patients have been included in clinical phase I-III tests showing significant reduction in alkaline...

  7. 31 CFR 223.22 - Fees for services of the Treasury Department.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Fees for services of the Treasury Department. 223.22 Section 223.22 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES...

  8. 18 CFR 2.23 - Use of reserved authority in hydropower licenses to ameliorate cumulative impacts.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Use of reserved authority in hydropower licenses to ameliorate cumulative impacts. 2.23 Section 2.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES...

  9. MiR-223 suppresses cell proliferation by targeting IGF-1R.

    Directory of Open Access Journals (Sweden)

    Cheng You Jia

    Full Text Available To study the roles of microRNA-223 (miR-223 in regulation of cell growth, we established a miR-223 over-expression model in HeLa cells infected with miR-223 by Lentivirus pLL3.7 system. We observed in this model that miR-223 significantly suppressed the proliferation, growth rate, colony formation of HeLa cells in vitro, and in vivo tumorigenicity or tumor formation in nude mice. To investigate the mechanisms involved, we scanned and examined the potential and putative target molecules of miR-223 by informatics, quantitative PCR and Western blot, and found that insulin-like growth factor-1 receptor (IGF-1R was the functional target of miR-223 inhibition of cell proliferation. Targeting IGF-1R by miR-223 was not only seen in HeLa cells, but also in leukemia and hepatoma cells. The downstream pathway, Akt/mTOR/p70S6K, to which the signal was mediated by IGF-1R, was inhibited as well. The relative luciferase activity of the reporter containing wild-type 3'UTR(3'untranslated region of IGF-1R was significantly suppressed, but the mutant not. Silence of IGF-1R expression by vector-based short hairpin RNA resulted in the similar inhibition with miR-223. Contrarily, rescued IGF-1R expression in the cells that over-expressed miR-223, reversed the inhibition caused by miR-223 via introducing IGF-1R cDNA that didn't contain the 3'UTR. Meanwhile, we also noted that miR-223 targeted Rasa1, but the downstream molecules mediated by Rasa1 was neither targeted nor regulated. Therefore we believed that IGF-1R was the functional target for miR-223 suppression of cell proliferation and its downstream PI3K/Akt/mTOR/p70S6K pathway suppressed by miR-223 was by targeting IGF-1R.

  10. Glut4 palmitoylation at Cys223 plays a critical role in Glut4 membrane trafficking.

    Science.gov (United States)

    Ren, Wenying; Sun, Yingmin; Du, Keyong

    2015-05-08

    Recently, we identified Glut4 as a palmitoylated protein in adipocytes. To understand the role of Glut4 palmitoylation in Glut4 membrane trafficking, a process that is essential for maintenance of whole body glucose homeostasis, we have characterized Glut4 palmitoylation. We found that Glut4 is palmitoylated at Cys223 and Glut4 palmitoylation at Cys223 is essential for insulin dependent Glut4 membrane translocation as substitution of Cys223 with a serine residue in Glut4 (C223S Glut4) diminished Glut4 responsiveness to insulin in membrane translocation in both adipocytes and CHO-IR cells. We have examined C223S Glut4 subcellular localization and observed that it was absence from tubular-vesicle structure, where insulin responsive Glut4 vesicles were presented. Together, our studies uncover a novel mechanism under which Glut4 palmitoylation regulates Glut4 sorting to insulin responsive vesicles, thereby insulin-dependent Glut4 membrane translocation.

  11. Cluster emission in the radioactive decay of 223Ac

    CERN Document Server

    Steyn, G F; Faccio, D; Bonetti, R; Tretyakova, S P; Shishkin, S V; Ogloblin, A A; Pik-Pichak, G A; Vermeulen, C; van der Meulen, N P; van der Walt, T N; McGee, D

    2010-01-01

    The branching ratio of 223Ac decay by spontaneous 14C emission was measured and a search for 15N clusters was performed. After exposure of a hemispherical array of solid-state nuclear track detectors, 347 14C events were identified and no 15N events. B(14C) = λ(14C)/λ(α) = (3.2 ± 1.0) x 10-11 is consistent with a favoured ground state to ground state transition. As no nitrogen tracks were found, only an upper limit could be inferred for 15N emission, B(15N) = λ(15N)/λ(α) ≤ 2.2 x 10-13 (confidence limit 90%), consistent with an unfavoured transition. Intense 227Pa sources were produced for this study, using the reaction 232Th(p,6n)227Pa. This offered an opportunity to compare the measured source strength with predictions based on published excitation function data.

  12. Daily temperature and precipitation data for 223 USSR Stations

    Energy Technology Data Exchange (ETDEWEB)

    Razuvaev, V.N.; Apasova, E.G.; Martuganov, R.A. [Research Institute of Hydrometeorological Information, Obninsk (Russian Federation); Vose, R.S. [Univ. of Tennessee, Knoxville, TN (United States); Steurer, P.M. [National Climatic Data Center, Asheville, NC (United States)

    1993-11-01

    On- May 23, 1972, the United States and the USSR established a bilateral initiative known as the Agreement on Protection of the Environment. Given recent interest in possible greenhouse gas-induced climate change, Working Group VIII (Influence of Environmental Changes on Climate) has become particularly useful to the scientific communities of both nations. Among its many achievements, Working Group VIII has been instrumental in the exchange of climatological information between the principal climate data centers of each country [i.e., the National Climatic Data Center (NCDC) in Asheville, North Carolina, and the Research Institute of Hydrometeorological Information in Obninsk, Russia]. Considering the relative lack of climate records previously available for the USSR, data obtained via this bilateral exchange are particularly valuable to researchers outside the former Soviet Union. To expedite the dissemination of these data, NOAA`s Climate and Global Change Program funded the Carbon Dioxide Information Analysis Center (CDIAC) and NCDC to distribute one of the more useful archives acquired through this exchange: a 223-station daily data set covering the period 1881-1989. This data set contains: (1) daily mean, minimum, and maximum temperature data; (2) daily precipitation data; (3) station inventory information (WMO No., name, coordinates, and elevation); (4) station history information (station relocation and rain gauge replacement dates); and (5) quality assurance information (i.e., flag codes that were assigned as a result of various data checks). The data set is available, free of charge, as a Numeric Data Package (NDP) from CDIAC. The NDP consists of 18 data files and a printed document which describes both the data files and the 223-station network in detail.

  13. 48 CFR 952.223-71 - Integration of environment, safety, and health into work planning and execution.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Integration of environment, safety, and health into work planning and execution. 952.223-71 Section 952.223-71 Federal Acquisition... Provisions and Clauses 952.223-71 Integration of environment, safety, and health into work planning...

  14. Efficient enantio- and diastereodivergent synthesis of poison-frog alkaloids 251O and trans-223B.

    Science.gov (United States)

    Toyooka, Naoki; Zhou, Dejun; Nemoto, Hideo; Tezuka, Yasuhiro; Kadota, Shigetoshi; Andriamaharavo, Nirina R; Garraffo, H Martin; Spande, Thomas F; Daly, John W

    2009-09-04

    An efficient and flexible synthesis of poison-frog alkaloids 251O and trans-223B has been achieved by using for both alkaloids an enantiodivergent process starting from the common lactam 1. The relative stereochemistry of 251O and trans-223B was determined to be 7 (R = n-C(7)H(15), R' = n-Pr) and 14 by the present enantioselective synthesis.

  15. MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue

    Directory of Open Access Journals (Sweden)

    Tung-Yueh Chuang

    2015-01-01

    Full Text Available MicroRNAs (miRNAs are short noncoding RNAs involved in posttranscriptional regulation of gene expression and influence many cellular functions including glucose and lipid metabolism. We previously reported that adipose tissue (AT from women with polycystic ovary syndrome (PCOS or controls with insulin resistance (IR revealed a differentially expressed microRNA (miRNA profile, including upregulated miR-93 in PCOS patients and in non-PCOS women with IR. Overexpressed miR-93 directly inhibited glucose transporter isoform 4 (GLUT4 expression, thereby influencing glucose metabolism. We have now studied the role of miR-223, which is also abnormally expressed in the AT of IR subjects. Our data indicates that miR-223 is significantly overexpressed in the AT of IR women, regardless of whether they had PCOS or not. miR-223 expression in AT was positively correlated with HOMA-IR. Unlike what is reported in cardiomyocytes, overexpression of miR-223 in human differentiated adipocytes was associated with a reduction in GLUT4 protein content and insulin-stimulated glucose uptake. In addition, our data suggests miR-223 regulates GLUT4 expression by direct binding to its 3′ untranslated region (3′UTR. In conclusion, in AT miR-223 is an IR-related miRNA that may serve as a potential therapeutic target for the treatment of IR-related disorders.

  16. MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis

    DEFF Research Database (Denmark)

    Løvendorf, Marianne B; Zibert, John R; Gyldenløve, Mette;

    2014-01-01

    including miR-223 and miR-143 which were found to be significantly upregulated in the PBMCs from patients with psoriasis compared with healthy controls (FCH=1.63, PmiR-223 and miR-143 significantly correlated with the PASIscore (r=0.46, P...miR-223 and -143 have the potential to distinguish between psoriasis and healthy controls (miR-223: area under the curve (AUC)=0.80, miR-143: AUC=0.75). Interestingly, after 3-5 weeks of treatment with methotrexate...... following a significant decrease in psoriasis severity, miR-223 and miR-143 were significantly downregulated in the PBMCs from patients with psoriasis. CONCLUSION: We suggest that changes in the miR-223 and miR-143 expressions in PBMCs from patients with psoriasis may serve as novel biomarkers for disease...

  17. AGN Feedback in Overdense Environments at z=2.23

    Science.gov (United States)

    Lucy, Adrian B.; Lehmer, B.; Alexander, D. M.; Best, P.; Geach, J.; Harrison, C. M.; Hornschemeier, A. E.; Matsuda, Y.; Mullaney, J.; Smail, I.; Sobral, D.

    2013-01-01

    We present results from a ≈100 ks Chandra observation of the 2QZ Cluster 1004+00 galaxy overdensity at z=2.23. This 2QZ Clus structure was first identified as an overdensity of four optically-selected quasars; that sample was subsequently found to overlap with an overdensity of 22 Hα-emitting galaxies (HAEs) identified through narrow and broad band near-infrared imaging by Matsuda et al. (2011). In addition to the preselected quasars in 2QZ Clus, our Chandra observation reveals that a further three HAEs are X-ray sources, all characterized by X-ray luminosities and spectral slopes consistent with unobscured active galactic nuclei (AGN). In total, we find that ≈30% of HAEs in our observed region of 2QZ Clus are AGN. This AGN fraction is high compared to AGN fractions among HAEs in the Chandra-COSMOS field (C-COSMOS), and if this enhancement is purely a result of the quasar selection bias of our sample, we estimate that such activity is rare at this redshift. Hα is a tracer of star formation, so 2QZ Clus is well suited to the investigation of the coeval growth of supermassive black holes and their host galaxies in the precursors to rich local clusters. Moreover, we have an ideal control sample in C-COSMOS; this survey contains a large sample of HAEs classified identically using infrared imaging, but without any selection of quasars. We calculate AGN fraction as a function of galaxy overdensity in C-COSMOS, and perform stacking analyses of Chandra and 250μ Herschel SPIRE data to obtain mean black hole accretion rates dMBH/dt and star formation rates SFR. Preliminary results indicate that dMBH/dt and its ratio to SFR are significantly elevated in 2QZ Clus compared to similarly overdense regions of C-COSMOS. We discuss these relations in the context of theoretical models describing the emergence of the MBH/Mgal relation of the local Universe.

  18. Dynamics of the X-ray clusters Abell 222, Abell 223 and Abell 520

    CERN Document Server

    Proust, D; Capelato, H V; Sodré, L; Lehodey, B T; Lefèvre, O; Mazure, A

    2000-01-01

    We present the results of a dynamical analysis of three rich, X-ray luminous galaxy clusters, Abell 222, Abell 223 and Abell 520, that are at intermediate redshifts. Our study is based on radial velocities for 71 cluster members, respectively 30 for A222, 20 for A223 and 21 for A520, measured from spectra obtained at the Canada-France-Hawaii Telescope, the European Southern Observatory, and the Pic du Midi Observatory, and supplemented with radial velocities from the literature. A222 galaxies have slightly higher velocities than those of A223, with bi-weighted mean velocity of V_{bi} = 64242 +/- 194 km/s for A222, and of V_{bi} = 63197 +/- 266 km/s for A223. The velocity dispersions of the two clusters are about the same: sigma_{bi} = 1013 +/- 150 km/s and sigma_{bi} = 1058 +/- 160 km/s for A222 and A223, respectively. For A520 we obtain V_{bi} = 60127 +/- 284 km/s with sigma_{bi} = 1250 +/- 189 km/s. We also give mass and mass-luminosities ratio estimates for each cluster separately. We argue that these clus...

  19. 脑卒中患者223例院内感染分析%Clinical Analysis of 223 Cerebral Apoplexy Patients catched Nosocomial infectious disease

    Institute of Scientific and Technical Information of China (English)

    蒋岚; 张小丽; 潘邑水

    2004-01-01

    目的预防脑卒中患者医院感染.方法对223例脑卒中医院感染进行回顾性分析.结果2 000例脑卒中患者中,医院内感染223例,感染率为11%,分别为呼吸道感染,泌尿系感染,消化道感染,皮肤软组织感染.结论脑卒中患者因自身特点,免疫力下降,侵入性操作,易发生医院感染.

  20. 36 CFR 223.113 - Modification of contracts to prevent environmental damage or to conform to forest plans.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Modification of contracts to prevent environmental damage or to conform to forest plans. 223.113 Section 223.113 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST...

  1. 12 CFR 223.23 - What valuation and timing principles apply to purchases of and investments in securities issued...

    Science.gov (United States)

    2010-01-01

    ....23 What valuation and timing principles apply to purchases of and investments in securities issued by... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What valuation and timing principles apply to purchases of and investments in securities issued by an affiliate? 223.23 Section 223.23 Banks and...

  2. 36 CFR 223.118 - Appeal process for small business timber sale set-aside program share recomputation decisions.

    Science.gov (United States)

    2010-07-01

    ... business timber sale set-aside program share recomputation decisions. 223.118 Section 223.118 Parks... business timber sale set-aside program share recomputation decisions. (a) Decisions subject to appeal. The... of the comment period, the Responsible Official shall make a decision on the small business...

  3. 12 CFR 223.43 - What are the standards under which the Board may grant additional exemptions from the...

    Science.gov (United States)

    2010-01-01

    ... bank seeks exemption; (2) Explain why the Board should exempt the transaction or relationship; and (3... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What are the standards under which the Board may grant additional exemptions from the requirements of section 23A? 223.43 Section 223.43 Banks...

  4. Circulating MicroRNA-223 Serum Levels Do Not Predict Sepsis or Survival in Patients with Critical Illness

    Directory of Open Access Journals (Sweden)

    Fabian Benz

    2015-01-01

    Full Text Available Background and Aims. Dysregulation of miR-223 was recently linked to various diseases associated with systemic inflammatory responses such as type 2 diabetes, cancer, and bacterial infections. However, contradictory results are available on potential alterations of miR-223 serum levels during sepsis. We thus aimed to evaluate the diagnostic and prognostic value of miR-223 serum concentrations in patients with critical illness and sepsis. Methods. We used i.v. injection of lipopolysaccharide (LPS as well as cecal pole ligation and puncture (CLP for induction of polymicrobial sepsis in mice and measured alterations in serum levels of miR-223. These results from mice were translated into a large and well-characterized cohort of critically ill patients admitted to the medical intensive care unit (ICU. Finally, results from analysis in patients were correlated with clinical data and extensive sets of routine and experimental biomarkers. Results. Although LPS injection induced moderately elevated serum miR-223 levels in mice, no significant alterations in miR-223 serum levels were found in mice after CLP-induced sepsis. In accordance with these results from animal models, serum miR-223 levels did not differ between critically ill patients and healthy controls. However, ICU patients with more severe disease (APACHE-II score showed moderately reduced circulating miR-223. Strikingly, no differences in miR-223 levels were found in critically ill patients with or without sepsis, and serum levels of miR-223 did not correlate with classical markers of inflammation or bacterial infection. Finally, low miR-223 serum levels were moderately associated with an unfavorable prognosis of patients during the ICU treatment but did not predict long-term mortality. Conclusion. Recent reports on alterations in miR-223 serum levels during sepsis revealed contradictory results, preventing a potential use of this miRNA in clinical routine. We clearly show that miR-223 serum

  5. 48 CFR 52.223-15 - Energy Efficiency in Energy-Consuming Products.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Energy Efficiency in... Provisions and Clauses 52.223-15 Energy Efficiency in Energy-Consuming Products. As prescribed in 23.206, insert the following clause: Energy Efficiency in Energy-Consuming Products (DEC 2007) (a) Definition....

  6. Imaging of Ra-223 with a small-pixel CdTe detector

    Science.gov (United States)

    Scuffham, J. W.; Pani, S.; Seller, P.; Sellin, P. J.; Veale, M. C.; Wilson, M. D.; Cernik, R. J.

    2015-01-01

    Ra-223 Dichloride (Xofigo™) is a promising new radiopharmaceutical offering survival benefit and palliation of painful bone metastases in patients with hormone-refractory prostate cancer [1]. The response to radionuclide therapy and toxicity are directly linked to the absorbed radiation doses to the tumour and organs at risk respectively. Accurate dosimetry necessitates quantitative imaging of the biodistribution and kinetics of the radiopharmaceutical. Although primarily an alpha-emitter, Ra-223 also has some low-abundance X-ray and gamma emissions, which enable imaging of the biodistribution in the patient. However, the low spectral resolution of conventional gamma camera detectors makes in-vivo imaging of Ra-223 challenging. In this work, we present spectra and image data of anthropomorphic phantoms containing Ra-223 acquired with a small-pixel CdTe detector (HEXITEC) [2] with a pinhole collimator. Comparison is made with similar data acquired using a clinical gamma camera. The results demonstrate the advantages of the solid state detector in terms of scatter rejection and quantitative accuracy of the images. However, optimised collimation is needed in order for the sensitivity to rival current clinical systems. As different dosage levels and administration regimens for this drug are explored in current clinical trials, there is a clear need to develop improved imaging technologies that will enable personalised treatments to be designed for patients.

  7. 39 CFR 223.2 - Channels of communication, headquarters with area offices.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Channels of communication, headquarters with area... RELATIONSHIPS AND COMMUNICATION CHANNELS § 223.2 Channels of communication, headquarters with area offices. (a) General. Headquarters organizational units formulate the directives to provide guidance to area...

  8. 48 CFR 52.223-12 - Refrigeration Equipment and Air Conditioners.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Refrigeration Equipment... Provisions and Clauses 52.223-12 Refrigeration Equipment and Air Conditioners. As prescribed in 23.804(b), insert the following clause: Refrigeration Equipment and Air Conditioners (MAY 1995) The Contractor...

  9. 30 CFR 250.223 - What mitigation measures information must accompany the EP?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What mitigation measures information must... INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Contents of Exploration Plans (ep) § 250.223 What mitigation measures information must accompany the EP?...

  10. 36 CFR 223.6 - Cutting and removal of timber in free-use areas.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Cutting and removal of timber... OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER General Provisions § 223.6 Cutting... or designated by forest officers may be cut and removed for personal use for domestic...

  11. 27 CFR 25.223 - Destruction of beer off brewery premises.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Destruction of beer off... TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Voluntary Destruction § 25.223 Destruction of beer off brewery premises. (a) Destruction without supervision. A brewer may destroy beer...

  12. 12 CFR 223.54 - What advertisements and statements are prohibited by section 23B?

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What advertisements and statements are... (REGULATION W) General Provisions of Section 23B § 223.54 What advertisements and statements are prohibited by section 23B? (a) In general. A member bank and its affiliates may not publish any advertisement or...

  13. 48 CFR 1252.223-73 - Seat belt use policies and programs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Seat belt use policies and....223-73 Seat belt use policies and programs. As prescribed in (TAR) 48 CFR 1223.7000(c), insert the following clause: Seat Belt Use Policies and Programs (APR 2005) In accordance with Executive Order...

  14. X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX

    Science.gov (United States)

    We report the first X-ray structure of a glycoside hydrolase family 43 ß-xylosidase, RS223BX, which is strongly activated by the addition of divalent metal cations. The 2.69 Å structure reveals that the Ca2+ cation is located at the back of the active site pocket. The Ca2+ coordinates to H274 to sta...

  15. 47 CFR 95.223 - (R/C Rule 23) Do I have to make my R/C station available for inspection?

    Science.gov (United States)

    2010-10-01

    ... available for inspection? 95.223 Section 95.223 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES PERSONAL RADIO SERVICES Radio Control (R/C) Radio Service Other Things You Need to Know § 95.223 (R/C Rule 23) Do I have to make my R/C station available for...

  16. Therapy palliative with {sup 2}23Ra without special radiation protection measures?; Palliative Therapie mit {sup 223}Ra ohne besondere Strahlenschutzmassnahmen?

    Energy Technology Data Exchange (ETDEWEB)

    Just, Guenther [Forschungsbuero Radonbalneologie (FRB), Grosspoesna (Germany); Petzold, Juergen

    2015-07-01

    For nearly 2 years now as a therapy of the castration resistant prostata carcinoma a nuclide therapy with {sup 223}Ra-Dichloride (trade-mark Xofigo) is applied. Xofigo is applied by a medical specialist for nuclear medicine altogether 6 times in a monthly distance. The activity used in each case is according to the body weight (50 kBq/kg BW). This therapy is licensed by the supervisory authorities of the German federal countries as an ambulant therapy. Special radiation protection measures are only required when exceeding a given number of 17 patients per year as incorparation measurements.

  17. Estimation of dose rate of a package ({sup 223+}Ra) and evaluation of transport index; Dosisleistungsabschaetzung bei einem Versandstueck ({sup 223+}Ra) und Ermittlung der Transportkennzahl nach ADR

    Energy Technology Data Exchange (ETDEWEB)

    Bittner, Michael [TUEV SUED Industrie Services GmbH, Region Nordost, Leipzig (Germany). Anlagensicherheit/Strahlenschutz; Richter, Jens [TUEV SUED Industrie Services GmbH, Region Nordost, Dresden (Germany). Anlagensicherheit/Strahlenschutz

    2016-08-01

    The transport index of a package is to be determined according to provisions of the ADR. It is directly related to the maximum radiation level in mSv/h at a distance of 1 m from the external surface of the package or pallet. To evaluate the existing distribution of the dose equivalent outside the package or pallet calculations of photon dose rates are required. For Monte-Carlo simulations with MCNP5 a three-dimensional model of a package containing Xofigo trademark was created, which contains all relevant sources from {sup 223}Ra and its decay chain.

  18. Factors affecting {sup 223}Ra therapy: clinical experience after 532 cycles from a single institution

    Energy Technology Data Exchange (ETDEWEB)

    Etchebehere, Elba C. [The University of Texas MD Anderson Cancer Center, Department of Nuclear Medicine, Houston, TX (United States); Campinas State University (Unicamp), Department of Nuclear Medicine, Campinas (Brazil); Milton, Denai R. [The University of Texas MD Anderson Cancer Center, Department of Biostatistics, Houston, TX (United States); Araujo, John C. [The University of Texas MD Anderson Cancer Center, Department of Genitourinary Medical Oncology, Houston, TX (United States); Swanston, Nancy M.; Macapinlac, Homer A.; Rohren, Eric M. [The University of Texas MD Anderson Cancer Center, Department of Nuclear Medicine, Houston, TX (United States)

    2016-01-15

    The aim of this study was to identify baseline features that predict outcome in {sup 223}Ra therapy. We retrospectively reviewed 110 patients with metastatic castration-resistant prostate cancer treated with {sup 223}Ra. End points were overall survival (OS), progression-free survival (PFS), bone event-free survival (BeFS), and bone marrow failure (BMF). The following parameters were evaluated prior to the first {sup 223}Ra cycle: serum levels of hemoglobin (Hb), prostate-specific antigen (PSA), alkaline phosphatase (ALP), Eastern Cooperative Oncology Group (ECOG) status, pain score, use of chemotherapy, and external beam radiation therapy (EBRT). During/after {sup 223}Ra we evaluated: the total number of radium cycles (Ra{sub Tot}), the PSA doubling time (PSA{sub DT}), and the use of chemotherapy, EBRT, abiraterone, and enzalutamide. A significant reduction of ALP (p < 0.001) and pain score (p = 0.041) occurred throughout the {sup 223} Ra cycles. The risk of progression was associated with declining ECOG status [hazard ratio (HR) = 3.79; p < 0.001] and decrease in PSA{sub DT} (HR = 8.22; p < 0.001). Ra{sub Tot}, ALP, initial ECOG status, initial pain score, and use of abiraterone were associated with OS (p ≤ 0.008), PFS (p ≤ 0.003), and BeFS (p ≤ 0.020). Ra{sub Tot}, ALP, initial ECOG status, and initial pain score were significantly associated with BMF (p ≤ 0.001) as well as Hb (p < 0.001) and EBRT (p = 0.009). On multivariable analysis, only Ra{sub Tot} and abiraterone remained significantly associated with OS (p < 0.001; p = 0.033, respectively), PFS (p < 0.001; p = 0.041, respectively), and BeFS (p < 0.001; p = 0.019, respectively). Additionally, Ra{sub Tot} (p = 0.027) and EBRT (p = 0.013) remained significantly associated with BMF. Concomitant use of abiraterone and {sup 223}Ra seems to have a beneficial effect, while the EBRT may increase the risk of BMF. (orig.)

  19. 225Ac and 223Ra production via 800 MeV proton irradiation of natural thorium targets.

    Science.gov (United States)

    Weidner, J W; Mashnik, S G; John, K D; Ballard, B; Birnbaum, E R; Bitteker, L J; Couture, A; Fassbender, M E; Goff, G S; Gritzo, R; Hemez, F M; Runde, W; Ullmann, J L; Wolfsberg, L E; Nortier, F M

    2012-11-01

    Cross sections for the formation of (225,227)Ac, (223,225)Ra, and (227)Th via the proton bombardment of natural thorium targets were measured at a nominal proton energy of 800 MeV. No earlier experimental cross section data for the production of (223,225)Ra, (227)Ac and (227)Th by this method were found in the literature. A comparison of theoretical predictions with the experimental data shows agreement within a factor of two. Results indicate that accelerator-based production of (225)Ac and (223)Ra is a viable production method.

  20. Kinetics of leptin binding to the Q223R leptin receptor.

    Directory of Open Access Journals (Sweden)

    Hans Verkerke

    Full Text Available Studies in human populations and mouse models of disease have linked the common leptin receptor Q223R mutation to obesity, multiple forms of cancer, adverse drug reactions, and susceptibility to enteric and respiratory infections. Contradictory results cast doubt on the phenotypic consequences of this variant. We set out to determine whether the Q223R substitution affects leptin binding kinetics using surface plasmon resonance (SPR, a technique that allows sensitive real-time monitoring of protein-protein interactions. We measured the binding and dissociation rate constants for leptin to the extracellular domain of WT and Q223R murine leptin receptors expressed as Fc-fusion proteins and found that the mutant receptor does not significantly differ in kinetics of leptin binding from the WT leptin receptor. (WT: ka 1.76×106±0.193×106 M-1 s-1, kd 1.21×10-4±0.707×10-4 s-1, KD 6.47×10-11±3.30×10-11 M; Q223R: ka 1.75×106±0.0245×106 M-1 s-1, kd 1.47×10-4±0.0505×10-4 s-1, KD 8.43×10-11±0.407×10-11 M. Our results support earlier findings that differences in affinity and kinetics of leptin binding are unlikely to explain mechanistically the phenotypes that have been linked to this common genetic variant. Future studies will seek to elucidate the mechanism by which this mutation influences susceptibility to metabolic, infectious, and malignant pathologies.

  1. Radio detection of XTE J1752-223 with the ATCA

    Science.gov (United States)

    Brocksopp, Catherine; Corbel, Stephane; Tzioumis, Tasso; Fender, Rob

    2009-11-01

    Following the detection of the new X-ray transient, XTE J1752-223, by RXTE and Swift (ATel. #2258; see also ATels. #2259, #2261, #2263, #2265, #2268, #2269), we have performed radio observations with the Australia Telescope Compact Array and the new CABB back-end. Data were obtained at 5.5 GHz and 9 GHz on October 30 and November 1. We detect a radio source at a position consistent with the X-ray coordinates.

  2. 微小RNA223在B淋巴增殖性疾病中的表达及其临床意义%Expression of microRNA-223 and its clinical value in B lymphoproliferative disorders

    Institute of Scientific and Technical Information of China (English)

    周可树; 宋永平; 邱录贵; 于珍; 易树华; 李增军; 安刚; 王燕婴; 邹德慧; 齐军元; 赵耀中

    2011-01-01

    目的 检测微小RNA (microRNA) 223在我国常见B淋巴增殖性疾病中的表达并分析其临床意义。方法 2003至2010年中国医学科学院血液病医院慢性淋巴细胞白血病(CLL)53例,套细胞淋巴瘤(MCL) 13例,脾边缘区淋巴瘤(SMZL)9例,健康对照12名。取外周血(78例)或骨髓(9例),常规分离单个核细胞并行CD19磁珠分选。提取CD19+B淋巴细胞的总RNA,应用TaqMan 探针法检测microRNA-223的表达。收集患者临床资料,应用SPSS 16.0软件进行统计学分析。结果 (1) microRNA-223的表达在CLL、MCL及SMZL分别为:4.58±0.62、4.03±0.54、4.63±0.57,显著低于健康对照(5.69±0.60,P<0.01);microRNA-223在MCL的表达显著低于CLL及SMZL(P<0. 05),而其在CLL与SMZL之间表达差异无统计学意义(P>0.05);(2)CLL患者microRNA-223的表达随疾病进展下调;CLL中IgVH未突变患者microRNA-223的表达水平显著低于IgVH突变患者(4. 05±0.69比4.67±0.51,P=0. 003);microRNA-223在13q-阳性患者的表达显著高于13q-阴性患者(4. 76±0.45比4.25±0.67,P=0. 044);(3)根据IgVH突变状态采用受试者工作特征(ROG)曲线方法,将microRNA-223的表达分为阳性组及阴性组。microRNA-223阳性组患者的中位疾病无进展生存(PFS)时间为48个月,高于microRNA-223阴性组的9个月(P =0.001),microRNA-223阳性组患者截至随访结束,无一例死亡。结论 micmRNA-223可能在B淋巴增殖性疾病的发病中起重要作用;其与患者预后相关,可能是CLL新的较为可靠的预后指标。%Objective To detect the expression of microRNA-223 and analyze its clinical value in B lymphoproliferative disorders. Methods Peripheral blood samples ( n = 78) and bone marrow samples ( n =9 ) were collected from patients with chronic lymphocytic leukemia ( CLL, n = 53 ), mantle cell lymphoma ( MCL, n = 13 ), splenic marginal zone lymphoma ( SMZL, n = 9) and healthy donors ( n = 12) at

  3. 40 CFR 421.223 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Science.gov (United States)

    2010-07-01

    ...) Molybdenum filtrate solvent extraction raffinate. BAT Limitations for the Secondary Molybdenum and Vanadium... Molybdenum and Vanadium Subcategory § 421.223 Effluent limitations guidelines representing the degree of.... BAT Limitations for the Secondary Molybdenum and Vanadium Subcategory Pollutant or pollutant...

  4. Possible cluster preformation in the 14C decay of 223Ra

    Science.gov (United States)

    Hussonnois, M.; Le Du, J. F.; Brillard, L.; Ardisson, G.

    1990-08-01

    A recent experiment on the 14C fine structure has shown that the branching ratio to 209Pb ground state is quite different from the one predicted by the various models of cluster emission. Assuming that the 14C is preformed in the 223Ra nucleus, we have calculated the hindrance factor F14C of the three 14C branches to 209Pb states with a barrier penetrability approximated by a square-well Coulomb potential. The F14C=3.9 and 4.6 values found for the 14C transitions to the two excited states at 779 keV (Iπ=11/2+) and 1423 keV (Iπ=15/2-), respectively, reveal that the cluster formation amplitudes in both transitions are close to those of the 222Ra and 224Ra 14C decays. Such values could be the result of ||nlj> shell-model components common to both the wave functions of the octupole deformed 223Ra nucleus and of the two first excited states of 209Pb spherical nucleus. The high hindrance factor F14C=583 of the 14C branch to the 209Pb ground state (Iπ=9/2+) could reveal the absence of ||1g9/2> shell-model component in the 223Ra wave function.

  5. Kepler-223: A Resonant Chain of Four Transiting, Sub-Neptune Planets

    Science.gov (United States)

    Mills, Sean; Fabrycky, Daniel C.; Migaszewski, Cezary; Ford, Eric B.; Petigura, Erik; Isaacson, Howard T.

    2016-05-01

    Surveys have revealed an abundance of multi-planet systems containing super-Earths and Neptunes in few-day to few-month orbits. Orbital periods of pairs of planets in the same system occasionally lie near, but generally not exactly on, ratios of small integers (resonances), allowing for the detection of the planets perturbing each other. There is debate whether in situ assembly or significant inward migration is the dominant mechanism of their formation. Simulations suggest migration creates tightly-packed, resonant systems, often in chains of resonance. Of the hundreds of multi-planet systems of sub-Neptunes, there is weak statistical enhancement near resonances, but no individual system has been identified that requires migration. Here we describe dynamical modeling of the system Kepler-223, which has a series of resonances among its four planets. We observe transit timing variations (TTVs), model them as resonant angle librations, and compute long-term stability, combining these analyses to constrain dynamical parameters and planetary masses. The detailed architecture of Kepler-223 is too finely tuned for formation by scattering, whereas numerical simulations demonstrate its properties are natural outcomes of the migration hypothesis. Similar systems could be destabilized by many mechanisms contributing to the observed period distribution. Planetesimal interactions in particular are thought to be responsible for establishing thecurrent orbits of the four giant planets in our own Solar System by disrupting a theoretical initial resonant chain like that actually observed in Kepler-223.

  6. Radium-223 chloride: a potential new treatment for castration-resistant prostate cancer patients with metastatic bone disease

    Directory of Open Access Journals (Sweden)

    Harrison MR

    2013-01-01

    Full Text Available Michael R Harrison, Terence Z Wong, Andrew J Armstrong, Daniel J GeorgeDuke Cancer Institute, Durham, NC, USABackground: Radium-223 chloride (223Ra; Alpharadin is an alpha-emitting radioisotope that targets areas of osteoblastic metastasis and is excreted by the small intestine. When compared with beta-emitters (eg, strontium-89, samarium-153, 223Ra delivers a high quantity of energy per track length with short tissue penetration.Objective: This review describes the mechanism, radiobiology, and preclinical development of 223Ra and discusses the clinical data currently available regarding its safety and efficacy profile.Methods: Data from clinical trials including abstracts were collected and reviewed using the PubMed Database, as well as the American Society of Clinical Oncology abstract database.Conclusion: Current bone-targeted therapies fall into two main categories: antiresorptive agents (eg, zoledronic acid, denosumab, which have been shown to delay skeletal-related events, and radiopharmaceuticals (eg, samarium-153, which may have a role in pain palliation. Historically, neither antiresorptive agents nor radiopharmaceuticals have shown definitive evidence of improved overall survival or other antitumor effects in metastatic castrate-resistant prostate cancer (mCRPC. Radiopharmaceuticals are limited by myelosuppresion, thrombocytopenia, and renal excretion. In a recently reported randomized Phase III trial in men with symptomatic bone-metastatic CRPC who had received or were ineligible for docetaxel chemotherapy, 223Ra treatment resulted in improved overall survival and delayed skeletal-related events. Toxicity consisted of minor gastrointestinal side effects and mild neutropenia and thrombocytopenia that were rarely severe. Pending regulatory approval, 223Ra may represent a unique and distinct option for an important subgroup of patients with mCRPC; future trials should address its use in combination or in sequence with existing and novel

  7. Study of 223Ra uptake mechanism by Fe3O4 nanoparticles: towards new prospective theranostic SPIONs

    Science.gov (United States)

    Mokhodoeva, Olga; Vlk, Martin; Málková, Eva; Kukleva, Ekaterina; Mičolová, Petra; Štamberg, Karel; Šlouf, Miroslav; Dzhenloda, Rustam; Kozempel, Ján

    2016-10-01

    The use of superparamagnetic iron oxide nanoparticles ( SPIONs) and radiolabelled nanoparticles ( NPs) has grown considerably over the recent years, and the SPIONs labelled with medicinal radionuclides offer new opportunities in multimodal diagnostics and in the drug-delivery systems for targeted alpha-particle therapy (TAT) driven by magnetic field gradient or by biologically active moieties bound on NPs shell. However, the mechanisms of NPs radiolabelling are not studied substantially and still remain unclear, even though the way of label attachment directly implies the stability of the label-nanoparticle construct. Since the 223Ra was the first clinically approved alpha-emitter, it is a promising nuclide for further development of its targeted carriers. We report here on the study of 223Ra uptake by the Fe3O4 SPIONs, together with an attempt to propose the 223Ra uptake mechanism by the Fe3O4 NPs in the presence of a phosphate buffer a typical formulation medium, under the pseudo-equilibrium conditions. Further, the in vitro stability tests of the prepared [223Ra]Fe3O4 NPs were performed to estimate the 223Ra label stability. The potential use of 223Ra-labelled SPIONs in theranostic applications is also discussed.

  8. Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

    Directory of Open Access Journals (Sweden)

    Yi Zhi

    2016-10-01

    Full Text Available Background/Aims: Ginkgolide B (GB is currently used as an anticancer drug for treatment of some malignant cancers. However, whether it may have therapeutic effects on bladder cancer remains unknown. Here, we studied the effects of GB on bladder cancer cells. Methods: Bladder cells were treated with different doses of GB, and the effects on ZEB1 and microRNA-223-3p (miR-223-3p were analyzed by RT-qPCR and/or Western blot. Prediction of a regulatory relationship between miR-93 and 3'-UTR of Beclin-1 mRNA was performed by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Results: We found that GB dose-dependently decreased ZEB1 protein, but not mRNA, in bladder cancer cells, resulting in suppression of cell invasion. Moreover, in bladder cancer cells, GB dose-dependently decreased the levels of miR-223-3p, which suppressed the protein translation of ZEB1 through binding to 3'-UTR of ZEB1 mRNA. Overexpression of miR-223-3p decreased ZEB1 protein, while depletion of miR-223-3p increased ZEB1 protein in bladder cancer cells. Conclusion: GB inhibits bladder cancer cell invasiveness through suppressing ZEB1 protein translation via upregulating miR-223-3p.

  9. miR-223 regulates cell growth and targets proto-oncogenes in mycosis fungoides/cutaneous T-cell lymphoma.

    Science.gov (United States)

    McGirt, Laura Y; Adams, Clare M; Baerenwald, Devin A; Zwerner, Jeffrey P; Zic, John A; Eischen, Christine M

    2014-04-01

    The pathogenesis of the cutaneous T-cell lymphoma (CTCL), mycosis fungoides (MF), is unclear. MicroRNA (miRNA) are small noncoding RNAs that target mRNA leading to reduced mRNA translation. Recently, specific miRNA were shown to be altered in CTCL. We detected significantly reduced expression of miR-223 in early-stage MF skin, and further decreased levels of miR-223 in advanced-stage disease. CTCL peripheral blood mononuclear cells and cell lines also had reduced miR-223 as compared with controls. Elevated expression of miR-223 in these cell lines reduced cell growth and clonogenic potential, whereas inhibition of miR-223 increased cell numbers. Investigations into putative miR-223 targets with oncogenic function, including E2F1 and MEF2C, and the predicted miR-223 target, TOX, revealed that all three were targeted by miR-223 in CTCL. E2F1, MEF2C, and TOX proteins were decreased with miR-223 overexpression, whereas miR-223 inhibition led to increased protein levels in CTCL. In addition, we showed that the 3'-UTR of TOX mRNA was a genuine target of miR-223. Therefore, reduced levels of miR-223 in MF/CTCL lead to increased expression of E2F1, MEF2C, and TOX, which likely contributes to the development and/or progression of CTCL. Thus, miR-223 and its targets may be useful for the development of new therapeutics for MF/CTCL.

  10. Relationship between arterial atheromatous plaque morphology and platelet-associated miR-126 and miR-223 expressions

    Institute of Scientific and Technical Information of China (English)

    Heng-Song Tian; Qing-Guo Zhou; Fang Shao

    2015-01-01

    Objective: To study the expression of miR-126 and miR-223 in platelet of rabbit arterial plaque models, and explore its correlation with plaque morphology. Methods: Rabbit arterial plaque models were established, peripheral blood of models and control animals was collected. Plaque morphologies were divided into type Ⅰ, type Ⅱ and type Ⅲ based on angiography plaque morphology and Ambrose method. Platelet isolation kit was applied to isolate and purify peripheral blood platelets, CD45 immunomagnetic beads were used to remove the residual white blood cells. The miRNAs of platelets was extracted by miRNA Isolation Kit, and expressions of miR-126 and miR-223 of the platelets samples were detected by Real-time PCR. The correlation between plaque morphology and platelet-associated miR-126 and miR-223 expressions were analyzed. Expressions of target gene VCAM-1 and P2Y12 receptors of miR-126 and miR-223 in the atherosclerosis plaque of rabbit model were detected by Western blot. Results: Relative expression levels of miR-126 and miR-223 in the model group were 0.27±0.10 and 0.71±0.14, respectively. Plaque morphology was divided into types Ⅰ, Ⅱ and Ⅲ;and miR-126 and miR-223 expression levels were detected in each type. Expression levels of miR-126 in each type were 0.42±0.07, 0.17±0.11 and 0.22±0.15, respectively; and expression levels of miR-223 in each type are 0.68±0.02, 0.57±0.06 and 0.88±0.10, respectively. Relative to the control group, miR-126 and miR-223 known target genes in VCAM-1 and P2Y12 receptors increased platelets in rabbit atherosclerotic plaque models (P<0.05). Conclusions:Relative to normal control animals, miR-126 and miR-223 platelets were reduced in the rabbit atherosclerotic plaque model group (P<0.05). In the type Ⅱ plaque morphology group, miR-126 was greatly reduced; and there is no significant correlation between miR-223 and plaque morphology.

  11. Inorganic phosphate accelerates the migration of vascular smooth muscle cells: evidence for the involvement of miR-223.

    Directory of Open Access Journals (Sweden)

    Ashraf Yusuf Rangrez

    Full Text Available BACKGROUND: An elevated serum inorganic phosphate (Pi level is a major risk factor for kidney disease and downstream vascular complications. We focused on the effect of Pi levels on human aortic vascular smooth muscle cells (VSMCs, with an emphasis on the role of microRNAs (miRNAs. METHODOLOGY/PRINCIPAL FINDINGS: Exposure of human primary VSMCs in vitro to pathological levels of Pi increased calcification, migration rate and concomitantly reduced cell proliferation and the amount of the actin cytoskeleton. These changes were evidenced by significant downregulation of miRNA-143 (miR-143 and miR-145 and concomitant upregulation of their targets and key markers in synthetic VSMCs, such as Krüppel-like factors-4 and -5 and versican. Interestingly, we also found that miR-223 (a marker of muscle damage and a key factor in osteoclast differentiation is expressed in VSMCs and is significantly upregulated in Pi-treated cells. Over-expressing miR-223 in VSMCs increased proliferation and markedly enhanced VSMC migration. Additionally, we found that the expression of two of the known miR-223 targets, Mef2c and RhoB, was highly reduced in Pi treated as well as miR-223 over-expressing VSMCs. To complement these in vitro findings, we also observed significant downregulation of miR-143 and miR-145 and upregulation of miR-223 in aorta samples collected from ApoE knock-out mice, which display vascular calcification. CONCLUSIONS/SIGNIFICANCE: Our results suggest that (i high levels of Pi increase VSMC migration and calcification, (ii altered expression levels of miR-223 could play a part in this process and (iii miR-223 is a potential new biomarker of VSMC damage.

  12. Effects of miR-223 on expression of IL-1β and IL-6 in human gingival fibroblasts.

    Science.gov (United States)

    Matsui, Sari; Ogata, Yorimasa

    2016-01-01

    MicroRNAs (miRNAs) are small non-coding RNAs that regulate post-transcriptional expression by translational inhibition or mRNA degradation. miRNAs bind to target mRNAs through partial complementarity, and can regulate many genes. In the present study, we investigated the effects of miR-223 on the expression of inflammatory cytokines in human gingival fibroblasts (HGF). To determine the effects of miR-223 on the expressions of interleukin-1β (IL-1β) and IL-6, HGF were stimulated by IL-1β (1 ng/mL) or tumor necrosis factor-α (TNF-α; 10 ng/mL) and transfected with a miR-223 expression plasmid. Levels of mRNA for IL-1β, IL-6, inhibitor of kappa-B kinase α (IKKα) and mitogen-activated protein kinase phosphatase-5 (MKP-5) were measured by real-time PCR, and levels IL-1β, IL-6 and IKKα protein were determined by enzyme-linked immunosorbent assay and Western blotting. Expression of IL-1β and IL-6 mRNAs was induced by IL-1β and TNF-α and further increased by miR-223 overexpression. IL-1β and TNF-α induced the expression of IL-1β and IL-6 mRNAs, and this was reduced by miR-223 inhibitor. Overexpression of miR-223 decreased the levels of IKKα protein and MKP-5 mRNA in HGF. These findings indicate that miR-223 might control the inflammatory response via IKKα and MKP-5 in periodontal tissue. (J Oral Sci 58, 101-108, 2016).

  13. Gln223Arg POLYMORPHISM IN LEPTIN RECEPTOR AND ITS ASSOCIATION WITH CEREBRAL INFARCTION AND ABDOMINAL OBESITY%瘦素受体基因Gln223Arg多态性与脑梗死和腹型肥胖关系

    Institute of Scientific and Technical Information of China (English)

    韩萌; 滕继军; 张文卿; 齐玉祥; 张金玉; 姚如永

    2012-01-01

    目的 探讨瘦素受体基因Gln223Arg多态性与脑梗死和腹型肥胖的相关性.方法 用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测295例脑梗死病人和256例健康对照人群(对照组)的瘦素受体基因Gln223Arg多态性;根据腹围标准将两组均分成腹型肥胖组和非腹型肥胖组,分析瘦素受体基因Gln223Arg多态性与脑梗死及腹型肥胖的关系.结果 脑梗死和对照组比较,基因型分布差异有显著性(x2=31.779,P<0.01),等位基因差异亦有显著性(OR=1.956,95%CI 1.527~2.506,x2=28.51,P<0.01).腹型肥胖与非腹型肥胖组基因型分布比较差异无统计学意义(x2 =4.475,P>0.05).结论 瘦素受体基因Gln223Arg多态性可能与脑梗死有关,G等位基因可能增加脑梗死发病风险,而与腹型肥胖无关.%Objective To explore the Gln223Arg polymorphism in leptin receptor (LEPR) and its correlation with cerebral infarction CCI) and abdominal obesity ( AO). Methods The Gln223 Arg polymorphism was studied by polymerase chain reaction and restrictive fragment length polymorphism (PCR-RFLP) in 295 patients with CI and 256 healthy controls. They were divided into AO group and non-AO group according to international standard of abdomen circumference. The correlation of Gln223Arg polymorphism with CI and AO was analyzed. Results The difference of distribution of genotype of Gln223Arg polymorphism in LEPR between CI and controls was significant (x2 = 31. 779, F0. 05). Conclusion Gln223Arg polymorphism in LEPR is possibly associated with cerebral infarction and not with abdominal obesity, and G allele is likely to increase the risk of cerebral infarction.

  14. Refinement of the deletion in 8q22.2-q22.3: the minimum deletion size at 8q22.3 related to intellectual disability and epilepsy.

    Science.gov (United States)

    Kuroda, Yukiko; Ohashi, Ikuko; Saito, Toshiyuki; Nagai, Jun-ichi; Ida, Kazumi; Naruto, Takuya; Iai, Mizue; Kurosawa, Kenji

    2014-08-01

    Kuechler et al. [2011] reported five patients with interstitial deletions in 8q22.2-q22.3 who had intellectual disability, epilepsy, and dysmorphic features. We report on a new patient with the smallest overlapping de novo deletion in 8q22.3 and refined the phenotype. The proposita was an 8-year-old girl, who developed seizures at 10 months, and her epileptic seizure became severe and difficult to control with antiepileptic drugs. She also exhibited developmental delay and walked alone at 24 months. She was referred to us for evaluation for developmental delay and epilepsy at the age of 8 years. She had intellectual disability (IQ 37 at 7 years) and autistic behavior, and spoke two word sentences at 8 years. She had mild dysmorphic features, including telecanthus and thick vermilion of the lips. Array comparative genomic hybridization detected a 1.36 Mb deletion in 8q22.3 that encompassed RRM2B and NCALD, which encode the small subunit of p53-inducible ribonucleotide reductase and neurocalcin delta in the neuronal calcium sensor family of calcium-binding proteins, respectively. The minimum overlapping region between the present and previously reported patients is considered to be a critical region for the phenotype of the deletion in 8q22.3. We suggest that the deletion in 8q22.3 may represent a clinically recognizable condition, which is characterized by intellectual disability and epilepsy.

  15. Carbon radioactivity of {sup 223}Ac and a search for nitrogen emission

    Energy Technology Data Exchange (ETDEWEB)

    Guglielmetti, A; Faccio, D; Bonetti, R [Istituto di Fisica Generale Applicata and INFN, Milano (Italy); Shishkin, S V; Tretyakova, S P [JINR, Dubna (Russian Federation); Dmitriev, S V; Ogloblin, A A; Pik-Pichak, G A [Kurchatov Institute, Moscow (Russian Federation); Meulen, N P van der; Steyn, G F; Walt, T N van der; Vermeulen, C; McGee, D [iThemba LABS, Somerset West 7129 (South Africa)], E-mail: Alessandra.Guglielmetti@mi.infn.it

    2008-05-15

    A very intense {sup 227}Pa source was produced in order to study the possible {sup 14}C and {sup 15}N spontaneous emission from {sup 223}Ac. After the irradiation of a hemispherical, highly efficient array of nuclear track detectors, about 350 Carbon events were found leading to a branching ratio with respect to alpha decay B = 3.2 10{sup -11}. Comparison with other {sup 14}C emitters allows the study of the influence of even-odd effects on cluster radioactivity.

  16. A Joint Source and Channel Video Coding Algorithmfor H.223 Based Wireless Channel

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Robust video streaming through high error-prone wireless channel has attracted much attention.In this paper the authors introduce an effective al gorithm by joining the Unequal Error Protection ability of the channel multiplexing prot ocol H.223 Annex D,and the new H.263++ Annex V Data Partition together. Bas ed on the optimal trade-off of these two technologies,the Joint Source and Channel Coding algorithm can result in stronger error resilience.The simulation results have shown its superiority against separate coding mode and some Unequal Error Protection mode under recommended wireless channel error patterns.

  17. ATCA detection of increased radio emission from XTE J1752-223

    Science.gov (United States)

    Brocksopp, Catherine; Corbel, Stephane; Tzioumis, Tasso; Fender, Rob; Coriat, Mickael

    2010-01-01

    Following the announcement that the new X-ray transient source, XTE J1752-223 (ATel. 2258), is making a transition from the hard to the intermediate state (ATel. 2387, 2391, 2396), we have observed the radio counterpart with the Australia Telescope Compact Array and the new CABB back-end. The radio source has risen from the previous ~2 mJy plateau to 20 mJy at 1.2, 2.5, 5.5, 9, 17, 19 GHz on January 21.

  18. Superconductivity in 2-2-3 system Y2Ba2Cu2O(8+delta)

    Science.gov (United States)

    Joshi, H. H.; Baldha, G. J.; Jotania, R. B.; Joshi, S. M.; Mohan, H.; Pandya, P. B.; Pandya, H. N.; Kulkarni, R. G.

    1991-01-01

    Researchers synthesized a new high T(sub c) 2-2-3 superconductor Y2Ba2Cu3O(8+delta) by a special preparation technique and characterized it by ac-susceptibility measurements. Diamagnetism and Meissner effect sets in at low fields and superconducting transition onsets at 90 K. The systematic investigation of the real and imaginary components of ac-susceptibility as a function of temperature and applied ac magnetic field reveals that the magnetic behavior is that of a granular type superconductor.

  19. Evolving treatment approaches for the management of metastatic castration-resistant prostate cancer – role of radium-223

    Directory of Open Access Journals (Sweden)

    Mukherji D

    2014-05-01

    Full Text Available Deborah Mukherji,1 Imane El Dika,1 Sally Temraz,1 Mohammed Haidar,2 Ali Shamseddine11Department of Hematology/Oncology, 2Department of Nuclear Medicine, American University of Beirut Medical Center, Beirut, LebanonAbstract: Radium-223 is a first-in-class alpha particle-emitting radiopharmaceutical approved for the treatment of bone metastatic castration-resistant prostate cancer. Radium-223 is administered intravenously with no requirement for complex shielding and specifically targets areas of bone metastasis. In a randomized placebo-controlled Phase III study, treatment with radium-223 was shown to improve overall survival, time to skeletal-related events, and health-related quality of life. Apart from radium-223, the cytotoxic chemotherapy agents docetaxel and cabazitaxel, androgen biosynthesis inhibitor abiraterone acetate, novel anti-androgen enzalutamide, and immunotherapy sipuleucel-T have also been shown to improve survival of men with advanced prostate cancer in Phase III trials. This review will outline current treatment approaches for advanced prostate cancer with a focus on the role of radium-223 in changing treatment paradigms.Keywords: Alpharadin, alpha-emitting radionuclide, bone metastasis

  20. Leptin receptor gene Gln223Arg polymorphism is not associated with obesity and metabolic syndrome in Turkish children.

    Science.gov (United States)

    Komşu-Ornek, Zuhal; Demirel, Fatma; Dursun, Ahmet; Ermiş, Bahri; Pişkin, Etem; Bideci, Aysun

    2012-01-01

    The aim of the study was to investigate the relationship between leptin receptor gene (LEPR) Gln223Arg polymorphism and obesity in Turkish children. Ninety-two obese and 99 lean children (between 5-15 years) were included in the study. Twenty-three of the obese children were diagnosed with metabolic syndrome. Blood samples were collected for morning fasting blood glucose, insulin, leptin, and lipid level measurements. LEPR Gln223Arg polymorphism was analyzed by restriction fragment length polymorphism. Significant differences were observed in anthropometric measurements, fasting blood glucose, insulin, leptin, and lipid levels between obese and lean children. Serum leptin levels were markedly higher in obese children. No significant association was noted between Gln223Arg polymorphism and serum leptin, insulin and lipid levels. There were no differences in the genotype frequencies or allele distribution for Gln223Arg polymorphism among obese, obese with metabolic syndrome and lean children. Our findings suggest that there is no association between Gln223Arg polymorphism and obesity in Turkish children.

  1. MicroRNA-223 Enhances Radiation Sensitivity of U87MG Cells In Vitro and In Vivo by Targeting Ataxia Telangiectasia Mutated

    Energy Technology Data Exchange (ETDEWEB)

    Liang, Liping; Zhu, Ji [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Zaorsky, Nicholas G. [Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania (United States); Deng, Yun [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Wu, Xingzhong [Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai (China); Liu, Yong [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Liu, Fangqi; Cai, Guoxiang; Gu, Weilie [Department of Colorectal Cancer, Fudan University, Shanghai Cancer Center, Shanghai (China); Shen, Lijun [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Zhang, Zhen, E-mail: zhenzhang6@hotmail.com [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China)

    2014-03-15

    Purpose: Ataxia telangiectasia mutated (ATM) protein is important in the DNA damage response because it repairs radiation-induced damage in cancers. We examined the effect of microRNA-223 (miR-223), a regulator of ATM expression, on radiation sensitivity of cancer cells. Methods and Materials: Human embryonic kidney 293 T (293T) cells were infected with pLL3.7-miR-223 plasmid to generate the pLL3.7-miR-223 and -empty virus (EV) lentivirus (miR-223 and EV). A dual luciferase assay in which the reporter contained wild-type 3′ untranslated region (UTR) of ATM was performed. U87MG cells were infected with miR-223 or EV to establish the overexpressed stable cell lines (U87-223 or U87-EV, respectively). Cells were irradiated in vitro, and dose enhancement ratios at 2 Gy (DER{sub 2}) were calculated. Hind legs of BALB/c athymic mice were injected with U87-223 or U87-EV cells; after 2 weeks, half of the tumors were irradiated. Tumor volumes were tracked for a total of 5 weeks. Results: The dual luciferase reporter assay showed a significant reduction in luciferase activity of 293T cells cotransfected with miR-223 and the ATM 3′UTR compared to that in EV control. Overexpression of miR-223 in U87MG cells showed that ATM expression was significantly downregulated in the U87-223 cells compared to that in U87-EV (ATM/β-actin mRNA 1.0 vs 1.5, P<.05). U87-223 cells were hypersensitive to radiation compared to U87-EV cells in vitro (DER{sub 2} = 1.32, P<.01). Mice injected with miR-223-expressing tumors had almost the same tumors after 3 weeks (1.5 cm{sup 3} vs 1.7 cm{sup 3}). However, irradiation significantly decreased tumor size in miR-223-expressing tumors compared to those in controls (0.033 cm{sup 3} vs 0.829 cm{sup 3}). Conclusions: miR-223 overexpression downregulates ATM expression and sensitizes U87 cells to radiation in vitro and in vivo. MicroRNA-223 may be a novel cancer-targeting therapy, although its cancer- and patient-specific roles are

  2. A Relativistic Many-Body Analysis of the Electric Dipole Moment of $^{223}$Rn

    CERN Document Server

    Sahoo, B K; Das, B P

    2014-01-01

    We report the results of our {\\it ab initio} relativistic many-body calculations of the electric dipole moment (EDM) $d_A$ arising from the electron-nucleus tensor-pseudotensor (T-PT) interaction, the interaction of the nuclear Schiff moment (NSM) with the atomic electrons and the electric dipole polarizability $\\alpha_d$ for $^{223}$Rn. Our relativistic random-phase approximation (RPA) results are substantially larger than those of lower-order relativistic many-body perturbation theory (MBPT) and the results based on the relativistic coupled-cluster (RCC) method with single and double excitations (CCSD) are the most accurate to date for all the three properties that we have considered. We obtain $d_A = 4.85(6) \\times 10^{-20} C_T \\ |e| \\ cm$ from T-PT interaction, $d_A=2.89(4) \\times 10^{-17} {S/(|e|\\ fm^3)}$ from NSM interaction and $\\alpha_d=35.27(9) \\ ea_0^3$. The former two results in combination with the measured value of $^{223}$Rn EDM, when it becomes available, could yield the best limits for the T-...

  3. Stochastic simulation of radium-223 dichloride therapy at the sub-cellular level

    Science.gov (United States)

    Gholami, Y.; Zhu, X.; Fulton, R.; Meikle, S.; El-Fakhri, G.; Kuncic, Z.

    2015-08-01

    Radium-223 dichloride (223Ra) is an alpha particle emitter and a natural bone-seeking radionuclide that is currently used for treating osteoblastic bone metastases associated with prostate cancer. The stochastic nature of alpha emission, hits and energy deposition poses some challenges for estimating radiation damage. In this paper we investigate the distribution of hits to cells by multiple alpha particles corresponding to a typical clinically delivered dose using a Monte Carlo model to simulate the stochastic effects. The number of hits and dose deposition were recorded in the cytoplasm and nucleus of each cell. Alpha particle tracks were also visualized. We found that the stochastic variation in dose deposited in cell nuclei (≃ 40%) can be attributed in part to the variation in LET with pathlength. We also found that ≃ 18% of cell nuclei receive less than one sigma below the average dose per cell (≃ 15.4 Gy). One possible implication of this is that the efficacy of cell kill in alpha particle therapy need not rely solely on ionization clustering on DNA but possibly also on indirect DNA damage through the production of free radicals and ensuing intracellular signaling.

  4. Low-frequency study of two giant radio galaxies: 3C 35 and 3C 223

    Science.gov (United States)

    Orrù, E.; Murgia, M.; Feretti, L.; Govoni, F.; Giovannini, G.; Lane, W.; Kassim, N.; Paladino, R.

    2010-06-01

    Aims: Radio galaxies with a projected linear size ⪆1 Mpc are classified as giant radio sources. According to the current interpretation these are old sources which have evolved in a low-density ambient medium. Because radiative losses are negligible at low frequency, extending spectral aging studies in this frequency range will allow us to determine the zero-age electron spectrum injected and then to improve the estimate of the synchrotron age of the source. Methods: We present Very Large Array images at 74 MHz and 327 MHz of two giant radio sources: 3C 35 and 3C 223. We performed a spectral study using 74, 327, 608 and 1400 GHz images. The spectral shape is estimated in different positions along the source. Results: The radio spectrum follows a power-law in the hotspots, while in the inner region of the lobe the shape of the spectrum shows a curvature at high frequencies. This steepening agrees with synchrotron aging of the emitting relativistic electrons. In order to estimate the synchrotron age of the sources, the spectra were fitted with a synchrotron model of emission. They show that 3C 35 is an old source of 143 ± 20 Myr, while 3C 223 is a younger source of 72 ± 4 Myr.

  5. 3-D Kinematics of the near-IR HH 223 outflow in L723

    CERN Document Server

    López, R; Estalella, R; Gómez, G; García-Lorenzo, B

    2014-01-01

    In this work we derive the full 3-D kinematics of the near-infrared outflow HH 223, located in the dark cloud Lynds 723 (L723), where a well-defined quadrupolar CO outflow is found. HH 223 appears projected onto the two lobes of the east-west CO outflow. The radio continuum source VLA 2, towards the centre of the CO outflow, harbours a multiple system of low-mass young stellar objects. One of the components has been proposed to be the exciting source of the east-west CO outflow. From the analisys of the kinematics, we get further evidence on the relationship between the near-infrared and CO outflows and on the location of their exciting source. The proper motions were derived using multi-epoch, narrow-band H$_2$ (2.122 $\\mu$m line) images. Radial velocities were derived from the 2.122 $\\mu$m line of the spectra. Because of the extended (~5 arcmin), S-shaped morphology of the target, the spectra were obtained with the Multi-Object-Spectroscopy (MOS) observing mode using the instrument LIRIS at the 4.2m William...

  6. 3D kinematics of the near-IR HH 223 outflow in L723

    Science.gov (United States)

    López, R.; Acosta-Pulido, J. A.; Estalella, R.; Gómez, G.; García-Lorenzo, B.

    2015-03-01

    In this work, we derive the full 3D kinematics of the near-infrared outflow HH 223, located in the dark cloud Lynds 723 (L723), where a well-defined quadrupolar CO outflow is found. HH 223 appears projected on to the two lobes of the east-west CO outflow. The radio continuum source VLA 2, towards the centre of the CO outflow, harbours a multiple system of low-mass young stellar objects. One of the components has been proposed to be the exciting source of the east-west CO outflow. From the analysis of the kinematics, we get further evidence on the relationship between the near-infrared and CO outflows and on the location of their exciting source. The proper motions were derived using multi-epoch, narrow-band H2 (2.122 μm line) images. Radial velocities were derived from the 2.122 μm line of the spectra. Because of the extended (˜5 arcmin), S-shaped morphology of the target, the spectra were obtained with the multi-object-spectroscopy (MOS) observing mode using the instrument Long-Slit Intermediate Resolution Infrared Spectrograph (LIRIS) at the 4.2 m William Herschel Telescope. To our knowledge, this work is the first time that MOS observing mode has been successfully used in the near-infrared range for an extended target.

  7. Relativistic many-body analysis of the electric dipole moment of 223Rn

    Science.gov (United States)

    Sahoo, B. K.; Singh, Yashpal; Das, B. P.

    2014-11-01

    We report the results of our ab initio relativistic many-body calculations of the electric dipole moment (EDM) dA arising from the electron-nucleus tensor-pseudotensor (T-PT) interaction, the interaction of the nuclear Schiff moment (NSM) with the atomic electrons and the electric dipole polarizability αd for 223Rn . Our relativistic random-phase approximation results are substantially larger than those of lower-order relativistic many-body perturbation theory and the results based on the relativistic coupled-cluster method with single and double excitations are highly accurate for all three properties that we have considered. We obtain dA=4.85 (6 ) ×10-20 CT|e | cm from T-PT interaction, dA=2.89 (4 ) ×10-17S /(|e |fm3) from NSM interaction, and αd=35.27 (9 ) e a03 . The former two results in combination with the measured value of 223Rn EDM, when it becomes available, could yield the best limits for the T-PT coupling constant, EDMs, and chromo-EDMs of quarks and θQCD parameter, and would thereby shed light on leptoquark and supersymmetric models that predict C P violation.

  8. LEPR Gene Gln223Arg Polymorphism in Chinese Families with Type 2Diabetes%2型糖尿病家系患者LEPR基因Gln223Arg多态性的研究

    Institute of Scientific and Technical Information of China (English)

    孙宏; 杨泽; 苗长青; 赵晓雯; 张慧娟; 李艺萍; 梁立波; 曹佳; 王月影; 孙亮

    2011-01-01

    目的:研究黑龙江地区汉族人2型糖尿病家系的LEPR基因Gln223Arg多态性,探讨其与2型糖尿病发病的关系.方法:应用聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)技术,对来自于黑龙江地区120个2型糖尿病家系中的210例2型糖尿病患者及319例正常对照的LEPR基因Gln223Arg (668 A→G)位点进行基因分型.结果:LEPR基因Gln223Arg三种基因型在病例组和对照组间整体分布有统计学意义(P=0.034,df=2);除AG基因型(x2=4.550,P<0.01)外,其余各基因型及等位基因在病例组和对照组间分布未见显著性差异(P>0.05).结论:LEPR基因Gln223Arg多态性与黑龙江地区汉族人2型糖尿病有关,LEPR基因可能为中国人2型糖尿病发病的相关易感基因.%Objective: To detect LEPR gene Gln223Arg polymorphism in Heilongjiang Han families with type 2 diabetes and to investigate their relationship with type 2 diabetes. Methods: PCR-RFLP method was used to test the polymorphisms of LEPR Gln223Arg (668 A-*G) in 210 individuals from 120 type 2 diabetes families and 319 normal control subjects in Heilongjiang area. Results: The overall distribution of the three genotypes of LEPR gene Gln223 Arg between cases and controls was significantly different (P=0.034, df=2). Except AG genotype (x2=.550, PO.01), the distribution of the rest of the genotypes and alleles between cases and controls had no significant difference (P>0.05). Conclusion: LEPR gene Gln223Arg polymorphism is related to type 2 diabetes in Heilongjiang Han nationality. LEPR gene may be a related gene of type 2 diabetes in Chinese.

  9. STRUCTURAL CONSEQUENCES OF THE POLYMORPHISM Q223R IN THE HUMAN LEPTIN RECEPTOR: A MOLECULAR DYNAMICS STUDY

    Directory of Open Access Journals (Sweden)

    Jonathan P. Carrillo-Vázquez

    2013-01-01

    Full Text Available Leptin Receptor (LEPR is a component of a signaling pathway related to appetite and energy expenditure. Single Nucleotide Polymorphisms (SNP of Leptin receptor gene (lepr have been proposed as possible modulator of adipose tissue and body weight. The main phenomenological consequence reported of these SNPs is the modulation of the LEP-LEPR interaction promoting the weight gain. Particularly, Q223R polymorphism has been associated with human obesity in some populations. In this work, we analyze the structural effects of Q223R substitution in a model of the extracellular region of LEPR comparing the stability between LEPR-Q and its Q223R variant (rs1137101 by Molecular Dynamics (MD simulations. These results showed different behavior between both molecules after one nanosecond (ns of simulation and significant differences in the secondary structure content were evidenced.

  10. Radium-223 dichloride: illustrating the benefits of a multidisciplinary approach for patients with metastatic castration-resistant prostate cancer

    Directory of Open Access Journals (Sweden)

    Renzulli II JF

    2015-06-01

    Full Text Available Joseph F Renzulli II, Jennifer Collins, Anthony Mega Genitourinary Multidisciplinary Clinic, The Miriam Hospital, Providence, RI, USAAbstract: Improving options for patients with metastatic castration-resistant prostate cancer (mCRPC provide latitude in designing treatment plans that meet patients' medical needs and personal goals. The field's rapid evolution opens avenues for contributions by multiple medical specialties and requires considering more options to ensure that each patient receives the most appropriate care. A multidisciplinary clinic (MDC focusing on patients with cancers of the genitourinary tract demonstrates an efficient and cost-effective means of integrating the diverse professional knowledge and skills needed to develop an optimal patient treatment plan. As a guide to establishing an MDC for patients with mCRPC, this article describes the operation of the Genitourinary MDC at The Miriam Hospital in Providence, RI – specifically, the successful incorporation of radium-223 dichloride (radium-223 into the treatment algorithm for men with mCRPC and symptomatic bone metastases. Radium-223 is a new treatment that, unlike earlier radionuclide therapies, has shown a survival advantage in a large randomized phase 3 trial (ALSYMPCA. The overall survival benefit was comparable to that of newer immuno- and hormonal therapies in similar populations. Radium-223 treatment also delayed onset of symptomatic skeletal events. Both benefits were independent of prior docetaxel therapy or concurrent bisphosphonate use. In our clinic, radium-223 is used primarily to extend patient survival. Patient selection, patient management, and treatment sequencing are discussed here in the context of a multidisciplinary environment. Keywords: radium-223 dichloride, prostate cancer, castration-resistant prostate cancer, multidisciplinary clinic, best practices

  11. Frontonasal dysplasia, callosal agenesis, basal encephalocele, and eye anomalies syndrome with a partial 21q22.3 deletion.

    Science.gov (United States)

    Guion-Almeida, Maria Leine; Richieri-Costa, Antonio; Jehee, Fernanda Sarquis; Passos-Bueno, Maria Rita Santos; Zechi-Ceide, Roseli Maria

    2012-07-01

    We describe a girl with a phenotype characterized by frontonasal dysplasia, callosal agenesis, basal encephalocele, and eye anomalies who presents a 46,XX,r(21) karyotype. Array-comparative genomic hybridization using the Afflymetrix 100K DNA oligoarray set showed an interstitial deletion 21q22.3 of approximately 219 kb. Conventional karyotype of both parents was normal, and it was not possible to perform the molecular studies. In this report we raise the hypothesis that the deleted genes located at 21q22.3 could account to the phenotype.

  12. Draft Genome of Multidrug-Resistant Klebsiella pneumoniae 223/14 Carrying KPC-6, Isolated from a General Hospital in Malaysia

    Science.gov (United States)

    Ahmad, Norazah; Chong, Teik Min; Hashim, Rohaidah; Shukor, Surianti; Yin, Wai-Fong; Chan, Kok-Gan

    2015-01-01

    We performed whole genome sequencing on a clinical multidrug-resistant Klebsiella pneumoniae strain 223/14. Investigation into its draft genome revealed the presence of KPC-6 variant, suggesting carbapenemase is present in this isolate. We found a plasmid-borne KPC gene (882 bp) inserted between two transposase genes in the genome of K. pneumoniae 223/14. PMID:26816553

  13. The black hole candidate XTE J1752-223 towards and in quiescence: optical and simultaneous X-ray-radio observations

    NARCIS (Netherlands)

    Ratti, E.M.; Jonker, P.G.; Miller-Jones, J.C.A.; Torres, M.A.P.; Homan, J.; Markoff, S.; Tomsick, J.; Kaaret, P.; Wijnands, R.; Gallo, E.; Özel, F.; Steeghs, D.T.H.; Fender, R.P.

    2012-01-01

    We present optical, X-ray and radio observations of the black hole transient (BHT) XTE J1752−223 towards and in quiescence. Optical photometry shows that the quiescent magnitude of XTE J1752−223 is fainter than 24.4 mag in the i′ band. A comparison with measurements of the source during its 2009-201

  14. Standardization Supports a Safer and More Harmonious Society——ISO/TC 223 Societal Security 12th Plenary Meeting Held in Beijing

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    The 12th Plenary Meeting of ISO/TC 223 (The Technical Committee of Societal Security)was held in Beijing from 7 to 11 November,2011.ISO/TC 223 is a newly-established and influential technical committee whose international standards of public safety provide significant technical support to the global societal security and economic development and are urgently needed.

  15. A transcription map of the 6p22.3 reading disability locus identifying candidate genes

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    Gruen Jeffrey R

    2003-06-01

    Full Text Available Abstract Background Reading disability (RD is a common syndrome with a large genetic component. Chromosome 6 has been identified in several linkage studies as playing a significant role. A more recent study identified a peak of transmission disequilibrium to marker JA04 (G72384 on chromosome 6p22.3, suggesting that a gene is located near this marker. Results In silico cloning was used to identify possible candidate genes located near the JA04 marker. The 2 million base pairs of sequence surrounding JA04 was downloaded and searched against the dbEST database to identify ESTs. In total, 623 ESTs from 80 different tissues were identified and assembled into 153 putative coding regions from 19 genes and 2 pseudogenes encoded near JA04. The identified genes were tested for their tissue specific expression by RT-PCR. Conclusions In total, five possible candidate genes for RD and other diseases mapping to this region were identified.

  16. 3C 223.1: A source with unusual spectral properties

    CERN Document Server

    Lal, D V; Lal, Dharam Vir

    2004-01-01

    Analysis of Giant Metrewave Radio Telescope low frequency data for an X-shaped source, 3C 223.1 has revealed an unusual result. The radio morphologies of it at 240 and 610 MHz show well defined X-shape with a pair of active jets along the north-south axis and a pair of wings along the east-west axis, that pass symmetrically through the undetected radio core. The wings (or low surface brightness jets) have flatter spectral indices with respect to the high surface brightness jets, which confirms the earlier marginal result obtained at high frequency by Dennett-Thorpe et al. (2002). Although unusual, it is a valuable result which puts stringent constraints on the formation models and nature of these sources. This result clearly shows the value of mapping the sample of X-shaped sources at low frequencies.

  17. Crystal Structure of D-Valine at the Temperature of 293, 270, 223 and 173K

    Institute of Scientific and Technical Information of China (English)

    WANG Wen-Qing; GONG Yan; WANG Zhe-Ming; YAN Chun-Hua

    2003-01-01

    The crystal structure of D-valine has been determined by Mo-Kα (λ = 0.71073 A) X-ray diffraction method for the first time. It crystallizes in monoclinic, space group P21 (Z = 4) with a = 9.6728(5), b = 5.2722(3), c = 12.0425(8) A, β = 90.750(2)° at 293 K. The asymmetric unit contains two crystallographically independent molecules — A (trans form) and B (gauche I form). They are rotational isomers with two kinds of conformations about the Cα -Cβ bond. The results of measurements of crystal structure at 293, 270, 223 and 173 K show a little change in bond lengths and geometries as a function of temperature. No evidence was obtained for the existence of configuration transformation from D- to L-valine.

  18. A Turner syndrome neurocognitive phenotype maps to Xp22.3

    Directory of Open Access Journals (Sweden)

    Elder Frederick F

    2007-05-01

    Full Text Available Abstract Background Turner syndrome (TS is associated with a neurocognitive phenotype that includes selective nonverbal deficits, e.g., impaired visual-spatial abilities. We previously reported evidence that this phenotype results from haploinsufficiency of one or more genes on distal Xp. This inference was based on genotype/phenotype comparisons of individual girls and women with partial Xp deletions, with the neurocognitive phenotype considered a dichotomous trait. We sought to confirm our findings in a large cohort (n = 47 of adult women with partial deletions of Xp or Xq, enriched for subjects with distal Xp deletions. Methods Subjects were recruited from North American genetics and endocrinology clinics. Phenotype assessment included measures of stature, ovarian function, and detailed neurocognitive testing. The neurocognitive phenotype was measured as a quantitative trait, the Turner Syndrome Cognitive Summary (TSCS score, derived from discriminant function analysis. Genetic analysis included karyotyping, X inactivation studies, fluorescent in situ hybridization, microsatellite marker genotyping, and array comparative genomic hybridization. Results We report statistical evidence that deletion of Xp22.3, an interval containing 31 annotated genes, is sufficient to cause the neurocognitive phenotype described by the TSCS score. Two other cardinal TS features, ovarian failure and short stature, as well as X chromosome inactivation pattern and subject's age, were unrelated to the TSCS score. Conclusion Detailed mapping suggests that haploinsufficiency of one or more genes in Xp22.3, the distal 8.3 megabases (Mb of the X chromosome, is responsible for a TS neurocognitive phenotype. This interval includes the 2.6 Mb Xp-Yp pseudoautosomal region (PAR1. Haploinsufficiency of the short stature gene SHOX in PAR1 probably does not cause this TS neurocognitive phenotype. Two genes proximal to PAR1 within the 8.3 Mb critical region, STS and NLGN4X, are

  19. G-2548A LEPTIN PROMOTER AND Q223R LEPTIN RECEPTOR POLYMORPHISMS IN OBESE MEXICAN SUBJECTS

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    P. Carrillo-Vazquez Jonathan

    2013-01-01

    Full Text Available Leptin interaction with its receptor in the hypothalamus stimulates a specific signaling cascade that results in the synthesis of anorexigenic and orexigenic peptides in order to regulate food intake and energy expenditure. Many polymorphisms in leptin (lep and leptin receptor (lepr genes have been associated with body weight. In particular, G-2548A in the lep promoter and Q223R in lepr variants have been associated with obesity in several populations, although no linkage has been evidenced in others. Here, we examined the genetic associations of these polymorphisms with Body Mass Index (BMI and serum leptin levels in adult Mexican people. A set of 160 subjects was recruited at the Acupuncture Clinic of the National School of Medicine and Homeopathy of the National Polytechnic Institute (Mexico and classified according to BMI, gender and age. Blood samples were obtained to extract genomic DNA and determine genetic variants by PCR-RFLP. Leptin was quantified by ELISA assays. Analysis of association and determination of Odd Ratio (OR were performed using SPSS software. G-2548A in lep gene promoter and Q223R in lepr gene polymorphisms were not found associated with BMI in the whole study population. However, GG genotype in lep gene promoter was related to an increased leptin concentration (p≤0.05 and suggested as a protective factor for obesity in Mexican women. Leptin levels were higher in postmenopausal women, confirming the link between the hormonal system and body weight control. In contrast, no association was found between lepr gene polymorphism and serum leptin level. Our results suggest a possible association between G-2548A polymorphism in lep gene promoter, BMI and leptin levels in Mexican women. Further analysis of a larger population is required to confirm the biological relevance of this polymorphism for obesity in the Mexican population.

  20. A 380-kb Duplication in 7p22.3 Encompassing the LFNG Gene in a Boy with Asperger Syndrome

    NARCIS (Netherlands)

    Vulto-van Silfhout, A.T.; Brouwer, A.F. de; Leeuw, N. de; Obihara, C.C.; Brunner, H.G.; Vries, B.B. de

    2012-01-01

    De novo genomic aberrations are considered an important cause of autism spectrum disorders. We describe a de novo 380-kb gain in band p22.3 of chromosome 7 in a patient with Asperger syndrome. This duplicated region contains 9 genes including the LNFG gene that is an important regulator of NOTCH sig

  1. Long-Term induction of defense gene expression in potato by Pseudomonas sp. LBUM223 and Streptomyces scabies

    NARCIS (Netherlands)

    Arseneault, T.; Pieterse, C.M.J.; Gérin-Ouellet, M.; Goyer, C.; Filion, M.

    2014-01-01

    Streptomyces scabies is a causal agent of common scab of potato, which generates necrotic tuber lesions. We have previously demonstrated that inoculation of potato plants with phenazine-1-carboxylic acid (PCA)-producing Pseudomonas sp. LBUM223 could significantly reduce common scab symptoms. In the

  2. Long-term induction of defense gene expression in potato by pseudomonas sp. LBUM223 and streptomyces scabies

    NARCIS (Netherlands)

    Arseneault, Tanya; Pieterse, Corné M J; Gérin-Ouellet, Maxime; Goyer, Claudia; Filion, Martin

    2014-01-01

    Streptomyces scabies is a causal agent of common scab of potato, which generates necrotic tuber lesions. We have previously demonstrated that inoculation of potato plants with phenazine-1-carboxylic acid (PCA)- producing Pseudomonas sp. LBUM223 could significantly reduce common scab symptoms. In the

  3. A late jet rebrightening revealed from multiwavelength monitoring of the black hole candidate XTE J1752-223

    NARCIS (Netherlands)

    Russell, D.M.; Curran, P.A.; Muñoz-Darias, T.; Lewis, F.; Motta, S.; Stiele, H.; Belloni, T.; Miller-Jones, J.C.A.; Jonker, P.G.; O'Brien, K.; Homan, J.; Casella, P.; Gandhi, P.; Soleri, P.; Markoff, S.; Maitra, D.; Gallo, E.; Cadolle Bel, M.

    2012-01-01

    We present optical monitoring of the black hole candidate XTE J1752−223 during its 2009-10 outburst and decay to quiescence. The optical light curve can be described by an exponential decay followed by a plateau, then a more rapid fade towards quiescence. The plateau appears to be due to an extra co

  4. 77 FR 52696 - Federal Acquisition Regulation; Information Collection; Drug-Free Workplace (FAR 52.223-6)

    Science.gov (United States)

    2012-08-30

    ... Regulation; Information Collection; Drug-Free Workplace (FAR 52.223-6) AGENCY: Department of Defense (DOD... approved information collection requirement concerning drug- free workplace. Public comments are... Information Collection 9000- 0101, Drug-Free Workplace, by any of the following methods:...

  5. 48 CFR 52.223-16 - IEEE 1680 Standard for the Environmental Assessment of Personal Computer Products.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false IEEE 1680 Standard for the... CONTRACT CLAUSES Text of Provisions and Clauses 52.223-16 IEEE 1680 Standard for the Environmental Assessment of Personal Computer Products. As prescribed in 23.706(b)(1), insert the following clause:...

  6. Leptin receptor Gln223Arg polymorphism and breast cancer risk in Nigerian women: A case control study

    Directory of Open Access Journals (Sweden)

    Anyanwu Stanley N

    2008-11-01

    Full Text Available Abstract Background Leptin, a 16 kDa polypeptide hormone, implicated in various physiological processes, exerts its action through the leptin receptor, a member of the class I cytokine receptor family. Both leptin and leptin receptor have recently been implicated in processes leading to breast cancer initiation and progression in animal models and humans. An A to G transition mutation in codon 223 in exon 6 of the leptin receptor gene, resulting in glutamine to arginine substitution (Gln223Arg, lies within the first of two putative leptin-binding regions and may be associated with impaired signaling capacity of the leptin receptor. This study was designed to assess the role of this polymorphism in breast cancer susceptibility in Nigerian women. Methods We utilized a polymerase chain reaction (PCR-based restriction fragment length polymorphism (RFLP assay to evaluate the association between the Gln223Arg polymorphism of the leptin receptor gene and breast risk in Nigeria in a case control study involving 209 women with breast cancer and 209 controls without the disease. Study participants were recruited from surgical outpatient clinics and surgical wards of four University Teaching Hospitals located in Midwestern and southeastern Nigeria between September 2002 and April 2004. Results Premenopausal women carrying at least one LEPR 223Arg allele were at a modestly increased risk of breast cancer after adjusting for confounders (OR = 1.8, 95% confidence interval [CI] 1.0–3.2, p = 0.07. There was no association with postmenopausal breast cancer risk (OR = 0.9, 95% CI 0.4–1.8, p = 0.68. Conclusion Our results suggest that the LEPR Gln223Arg polymorphism in the extracellular domain of the LEPR receptor gene is associated with a modestly increased risk of premenopausal breast cancer in Nigerian women.

  7. 奶牛OLR1 3′UTR A223C多态的生物学信息分析%Bioinformatics Analysis of A223C Polymorphism at the 3'UTR of OLR1 Gene in Cow

    Institute of Scientific and Technical Information of China (English)

    张传生; 耿立英; 尹春光; 曹顶国; 刘铮铸; 付志新

    2011-01-01

    [目的]探索氧化型低密度脂蛋白受体OLR1 3′UTR A223C 多态与奶牛乳脂率高低紧密关联的分子机制.[方法]用miRanda(1.0)软件装载牛microRNA数据库,预测OLR1 3′UTR潜在的microRNA.[结果]共预测16个microRNA靶标,OLR1 3′UTR A223C突变定位于bta-miR-370 靶标种子序列的互补区.[结论]由于OLR1 3′UTR A→C突变导致bta-miR-370 靶标的消失,为OLR1 3′UTR A223C 多态与奶牛乳脂率高低紧密关联的分子机制深入研究提供依据.%[ Objective ] The aim was to explore the molecular mechanisms ot the A223C polymorphism at 3 'UTR of oxidized low density lipoprotein receptor( OLR1 )which was closely related to milk fat percentage in cow. [ Method]The putative microRNA targets located in the 3'UTR of OLR1 were predicted with the soft miRanda (1.0) which could carry the microRNA dateabase of cow. [ Result ] Total 16 microRNA targets were found. The .A223 C SNP was located in complementary region of bta-miR-370 target sequence. [ Conclusion ] OLR1 3'UTR A→C mutation had lead to the disappearing of bta-miR-370 target, which had provide basis for the further research on the molecular mechanisms of the A223C polymorphism at 3'UTR of OLR1 gene which was closely related to milk fat percentage in cow.

  8. Oxidized Low-density Lipoprotein (ox-LDL) Cholesterol Induces the Expression of miRNA-223 and L-type Calcium Channel Protein in Atrial Fibrillation

    Science.gov (United States)

    He, Fengping; Xu, Xin; Yuan, Shuguo; Tan, Liangqiu; Gao, Lingjun; Ma, Shaochun; Zhang, Shebin; Ma, Zhanzhong; Jiang, Wei; Liu, Fenglian; Chen, Baofeng; Zhang, Beibei; Pang, Jungang; Huang, Xiuyan; Weng, Jiaqiang

    2016-08-01

    Atrial fibrillation (AF) is the most common sustained arrhythmia causing high morbidity and mortality. While changing of the cellular calcium homeostasis plays a critical role in AF, the L-type calcium channel α1c protein has suggested as an important regulator of reentrant spiral dynamics and is a major component of AF-related electrical remodeling. Our computational modeling predicted that miRNA-223 may regulate the CACNA1C gene which encodes the cardiac L-type calcium channel α1c subunit. We found that oxidized low-density lipoprotein (ox-LDL) cholesterol significantly up-regulates both the expression of miRNA-223 and L-type calcium channel protein. In contrast, knockdown of miRNA-223 reduced L-type calcium channel protein expression, while genetic knockdown of endogenous miRNA-223 dampened AF vulnerability. Transfection of miRNA-223 by adenovirus-mediated expression enhanced L-type calcium currents and promoted AF in mice while co-injection of a CACNA1C-specific miR-mimic counteracted the effect. Taken together, ox-LDL, as a known factor in AF-associated remodeling, positively regulates miRNA-223 transcription and L-type calcium channel protein expression. Our results implicate a new molecular mechanism for AF in which miRNA-223 can be used as an biomarker of AF rheumatic heart disease.

  9. 6p22.3 amplification as a biomarker and potential therapeutic target of advanced stage bladder cancer

    Science.gov (United States)

    Zhang, Jianmin; Underwood, Willie; Yang, Nuo; Frangou, Costa; Eng, Kevin; Head, Karen; Bollag, Roni J.; Kavuri, Sravan K.; Rojiani, Amyn M.; Li, Yingwei; Yan, Li; Hill, Annette; Woloszynska-Read, Anna; Wang, Jianmin; Liu, Song; Trump, Donald L.; Candace, Johnson S.

    2013-01-01

    Genetic and epigenetic alterations have been identified as to contribute directly or indirectly to the generation of transitional cell carcinoma of the urinary bladder (TCC-UB). In a comparative fashion much less is known about copy number alterations in TCC-UB, but it appears that amplification of chromosome 6p22 is one of the most frequent changes. Using fluorescence in situ hybridization (FISH) analyses, we evaluated chromosomal 6p22 amplification in a large cohort of bladder cancer patients with complete surgical staging and outcome data. We have also used shRNA knockdown candidate oncogenes in the cell based study. We found that amplification of chromosome 6p22.3 is significantly associated with the muscle-invasive transitional cell carcinoma of the urinary bladder (TCC-UB) (22%) in contrast to superficial TCC-UB (9%) (p=7.2-04). The rate of 6p22.3 amplification in pN>1 patients (32%) is more than twice that in pN0 (16%) patients (p=0.05). Interestingly, we found that 6p22.3 amplification is as twice as high (p=0.0201) in African American (AA) than European American (EA) TCC-UB patients. Moreover, we showed that the expression of some candidate genes (E2F3, CDKAL1 and Sox4) in the 6p22.3 region is highly correlated with the chromosomal amplification. In particular, knockdown of E2F3 inhibits cell proliferation in a 6p22.3-dependent manner, whereas knockdown of CDKAL1 and Sox4 has no effect on cell proliferation. Using gene expression profiling, we further identified some common as well as distinctive subset targets of the E2F3 family members. In summary, our data indicate that E2F3 is a key regulator of cell proliferation in a subset of bladder cancer and the 6p22.3 amplicon is a biomarker of aggressive phenotype in this tumor type. PMID:24231253

  10. Proton-induced cross sections relevant to production of 225Ac and 223Ra in natural thorium targets below 200 MeV.

    Science.gov (United States)

    Weidner, J W; Mashnik, S G; John, K D; Hemez, F; Ballard, B; Bach, H; Birnbaum, E R; Bitteker, L J; Couture, A; Dry, D; Fassbender, M E; Gulley, M S; Jackman, K R; Ullmann, J L; Wolfsberg, L E; Nortier, F M

    2012-11-01

    Cross sections for (223,)(225)Ra, (225)Ac and (227)Th production by the proton bombardment of natural thorium targets were measured at proton energies below 200 MeV. Our measurements are in good agreement with previously published data and offer a complete excitation function for (223,)(225)Ra in the energy range above 90 MeV. Comparison of theoretical predictions with the experimental data shows reasonable-to-good agreement. Results indicate that accelerator-based production of (225)Ac and (223)Ra below 200 MeV is a viable production method.

  11. Proton-induced cross sections relevant to production of 225Ac and 223Ra in natural thorium targets below 200 MeV

    CERN Document Server

    Weidner, J W; John, K D; Hemez, F; Ballard, B; Bach, H; Birnbaum, E R; Bitteker, L J; Couture, A; Dry, D; Fassbender, M E; Gulley, M S; Jackman, K R; Ullmann, J L; Wolfsberg, L E; Nortier, F M

    2012-01-01

    Cross sections for 223,225Ra, 225Ac and 227Th production by the proton bombardment of natural thorium targets were measured at proton energies below 200 MeV. Our measurements are in good agreement with previously published data and offer a complete excitation function for 223,225Ra in the energy range above 90 MeV. Comparison of theoretical predictions with the experimental data shows reasonable-to-good agreement. Results indicate that accelerator-based production of 225Ac and 223Ra below 200 MeV is a viable production method.

  12. Turner syndrome mosaicism: an unusual case with a de novo large dicentric marker chromosome: mos 45,X/46,X, ter rea(X;X)(p22.3;p22.3).

    Science.gov (United States)

    Nucaro, Anna Lisa; Melis, Paola; Casini, Maria Rosaria; Rossino, Rossano; Cau, Milena; Melis, Maria Antonietta; Loche, Sandro

    2008-01-01

    X/X translocations are quite rare in humans. The effect of this anomaly on the phenotype is variable and depends on the amount of deleted material and whether the chromosomes are joined by their long or short arms. We report an unusual case of Turner syndrome mosaicism in a 16-year-old girl, who was referred to our Institute for primary amenorrhoea associated with short stature. Endocrine evaluation revealed hypergonadotropic hypogonadism, which required a study of the karyotype. Cytogenetic analysis, performed on peripheral blood leucocytes, showed a mos 45,X/46,X,ter rea (X;X)(p22.3;p22.3) de novo karyotype. The prevalent cell line was 45,X (90% cells). A second cell line (10% cells) showed a very large marker chromosome, similar to a large metacentric chromosome. FISH (fluorescent in situ hybridisation) and molecular analysis revealed that the marker chromosome was dicentric and totally derived from the paternal X chromosome.

  13. Radiation dosimetry in digital breast tomosynthesis: report of AAPM Tomosynthesis Subcommittee Task Group 223.

    Science.gov (United States)

    Sechopoulos, Ioannis; Sabol, John M; Berglund, Johan; Bolch, Wesley E; Brateman, Libby; Christodoulou, Emmanuel; Flynn, Michael; Geiser, William; Goodsitt, Mitchell; Jones, A Kyle; Lo, Joseph Y; Maidment, Andrew D A; Nishino, Kazuyoshi; Nosratieh, Anita; Ren, Baorui; Segars, W Paul; Von Tiedemann, Miriam

    2014-09-01

    The radiation dose involved in any medical imaging modality that uses ionizing radiation needs to be well understood by the medical physics and clinical community. This is especially true of screening modalities. Digital breast tomosynthesis (DBT) has recently been introduced into the clinic and is being used for screening for breast cancer in the general population. Therefore, it is important that the medical physics community have the required information to be able to understand, estimate, and communicate the radiation dose levels involved in breast tomosynthesis imaging. For this purpose, the American Association of Physicists in Medicine Task Group 223 on Dosimetry in Tomosynthesis Imaging has prepared this report that discusses dosimetry in breast imaging in general, and describes a methodology and provides the data necessary to estimate mean breast glandular dose from a tomosynthesis acquisition. In an effort to maximize familiarity with the procedures and data provided in this Report, the methodology to perform the dose estimation in DBT is based as much as possible on that used in mammography dose estimation.

  14. Six- and three-hourly meteorological observations from 223 USSR stations

    Energy Technology Data Exchange (ETDEWEB)

    Razuvaev, V.N.; Apasova, E.B.; Martuganov, R.A. [All-Russian Research Inst. of Hydrometeorologicl Information, Obninsk (Russia). World Data Centre; Kaiser, D.P. [Oak Ridge National Lab., TN (United States)

    1995-04-01

    This document describes a database containing 6- and 3-hourly meteorological observations from a 223-station network of the former Soviet Union. These data have been made available through cooperation between the two principal climate data centers of the United States and Russia: the National Climatic Data Center (NCDC), in Asheville, North Carolina, and the All-Russian Research Institute of Hydrometeorological Information -- World Data Centre (RIHMI-WDC) in Obninsk. Station records consist of 6- and 3-hourly observations of some 24 meteorological variables including temperature, weather type, precipitation amount, cloud amount and type, sea level pressure, relative humidity, and wind direction and speed. The 6-hourly observations extend from 1936 to 1965; the 3-hourly observations extend from 1966 through the mid-1980s (1983, 1984, 1985, or 1986; depending on the station). These data have undergone extensive quality assurance checks by RIHMI-WDC, NCDC, and the Carbon Dioxide Information Analysis Center (CDIAC). The database represents a wealth of meteorological information for a large and climatologically important portion of the earth`s land area, and should prove extremely useful for a wide variety of regional climate change studies. These data are available free of charge as a numeric data package (NDP) from CDIAC. The NDP consists of this document and 40 data files that are available via the Internet or on 8mm tape. The total size of the database is {approximately}2.6 gigabytes.

  15. Studying Gender in Conference Talks -- data from the 223rd meeting of the American Astronomical Society

    CERN Document Server

    Davenport, James R A; Grand, Erin; Hagen, Alex; Poppenhaeger, Katja; Watkins, Laura L

    2014-01-01

    We present a study on the gender balance, in speakers and attendees, at the recent major astronomical conference, the American Astronomical Society meeting 223, in Washington, DC. We conducted an informal survey, yielding over 300 responses by volunteers at the meeting. Each response included gender data about a single talk given at the meeting, recording the gender of the speaker and all question-askers. In total, 225 individual AAS talks were sampled. We analyze basic statistical properties of this sample. We find that the gender ratio of the speakers closely matched the gender ratio of the conference attendees. The audience asked an average of 2.8 questions per talk. Talks given by women had a slightly higher number of questions asked (3.2$\\pm$0.2) than talks given by men (2.6$\\pm$0.1). The most significant result from this study is that while the gender ratio of speakers very closely mirrors that of conference attendees, women are under-represented in the question-asker category. We interpret this to be a...

  16. Radiation dosimetry in digital breast tomosynthesis: Report of AAPM Tomosynthesis Subcommittee Task Group 223

    Energy Technology Data Exchange (ETDEWEB)

    Sechopoulos, Ioannis, E-mail: isechop@emory.edu [Departments of Radiology and Imaging Sciences, Hematology and Medical Oncology and Winship Cancer Institute, Emory University, 1701 Uppergate Drive Northeast, Suite 5018, Atlanta, Georgia 30322 (United States); Sabol, John M. [GE Healthcare, Global Diagnostic X-Ray, Mailstop W-701, 3000 North Grandview Boulevard, Waukesha, Wisconsin 53188 (United States); Berglund, Johan [Research and Development, Philips Women' s Healthcare, Solna (Sweden); Bolch, Wesley E. [J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611 (United States); Brateman, Libby [University of Florida, Gainesville, Florida 32611 (United States); Christodoulou, Emmanuel; Goodsitt, Mitchell [Department of Radiology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States); Flynn, Michael [Department of Radiology, Henry Ford Health System, Radiology Research 2F, 1 Ford Place, Detroit, Michigan 48202 (United States); Geiser, William [Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030-4009 (United States); Kyle Jones, A. [Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030 (United States); Lo, Joseph Y.; Paul Segars, W. [Department of Radiology, Medical Physics Graduate Program, and Department of Biomedical Engineering, Carl E. Ravin Advanced Imaging Laboratories, Duke University, Durham, North Carolina 27705 (United States); Maidment, Andrew D. A. [Department of Radiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania 19104-4206 (United States); Nishino, Kazuyoshi [R and D X-ray Products Group, Shimadzu Corporation, Tokyo (Japan); Nosratieh, Anita [Biomedical Engineering Graduate Group, Department of Radiology, University of California, Davis, California 95817 (United States); and others

    2014-09-15

    The radiation dose involved in any medical imaging modality that uses ionizing radiation needs to be well understood by the medical physics and clinical community. This is especially true of screening modalities. Digital breast tomosynthesis (DBT) has recently been introduced into the clinic and is being used for screening for breast cancer in the general population. Therefore, it is important that the medical physics community have the required information to be able to understand, estimate, and communicate the radiation dose levels involved in breast tomosynthesis imaging. For this purpose, the American Association of Physicists in Medicine Task Group 223 on Dosimetry in Tomosynthesis Imaging has prepared this report that discusses dosimetry in breast imaging in general, and describes a methodology and provides the data necessary to estimate mean breast glandular dose from a tomosynthesis acquisition. In an effort to maximize familiarity with the procedures and data provided in this Report, the methodology to perform the dose estimation in DBT is based as much as possible on that used in mammography dose estimation.

  17. Transient Relativistic Ejections and Stationary Core in XTE J1752-223

    CERN Document Server

    Yang, J; Corbel, S; Gurvits, L I; Campbell, R M; Brocksopp, C

    2011-01-01

    The Galactic X-ray transient XTE J1752-223 was shown to have properties of black hole binary candidates. As reported in our previous paper, we identified transient and decelerating ejecta in multi-epoch Very Long Baseline Interferometry (VLBI) observations with the European VLBI Network (EVN) and the NRAO Very Long Baseline Array (VLBA). Here we present new EVN and VLBA data in which a new transient ejection event and later a stationary component are identified. The latter is interpreted as a reappearance of the radio core/compact jet during the transition from soft to hard X-ray state. This component appears to be highly variable in brightness although effects of tropospheric instabilities might play a role too. We also re-analyze the earlier VLBI data and find that the transient ejecta closer to the core position has significantly higher proper motion, further strengthening the case for strongly decelerating ejecta on the scale of several hundred milli-arcsecond, never observed in X-ray binaries before. Alt...

  18. Identification of a genetic locus for ichthyosis vulgaris on chromosome 10q22.3-q24.2.

    Science.gov (United States)

    Liu, Ping; Yang, Qingyu; Wang, Xu; Feng, Aiping; Yang, Tao; Yang, Rong; Wang, Pengyun; Yuang, Mingxiong; Liu, Mugen; Liu, Jing Yu; Wang, Qing K

    2008-06-01

    Ichthyosis vulgaris (IV) is one of the most commonly inherited disorders and has an estimated prevalence rate of 2.29% in China. To date, only one gene responsible for IV, the filaggrin gene (FLG), was identified, but genetic heterogeneity exists. In this study, two Chinese families with autosomal-dominant IV were genetically characterized. The FLG gene was first excluded as the disease-causing gene in the two families. The larger family was then characterized by genome-wide linkage analysis to identify a new genetic locus for IV. Significant linkage was identified with markers on chromosome 10q22.3-q24.2 with a maximum LOD score of 3.19. No other markers showed a LOD score of >1.5. Fine mapping defined the new genetic locus within a 20.7 cM region between markers D10S569 and D10S1709. The second family also showed positive linkage to the same 10q22.3-q24.2 region. The combined maximum LOD score in the two families was 3.95. Identification of linkage in two independent families provides strong genetic evidence that a previously unreported gene for IV is located on chromosome 10q22.3-q24.2. Future studies of the candidate genes at the 10q IV locus will identify a specific gene, which will provide insights into the pathogenesis of IV.

  19. Discovery and X-ray Monitoring of a New Black Hole Candidate XTE J1752-223

    Science.gov (United States)

    Shaposhnikov, Nikolai; Swank, J. H.; Markwardt, C. B.; Krimm, H.

    2010-03-01

    On October 23, 2009 a new X-ray transient source XTE J1752-223 was discovered by RXTE during observations scanning the Galactic Bulge region. Source identification in the optical, infra-red, and radio immediately followed. The first pointed RXTE observation, three days after the discovery, revealed a very hard non-thermal energy spectrum and strong iron line emission. After the initial rise the source flux leveled off and showed very stable properties for more than a month of monitoring observations until the Sun was too close for RXTE to observe. We analyzed RXTE data collected during this monitoring campaign. The aperiodic fast variability properties are strongly reminiscent of the extreme hard states shown by the well known black hole binary Cygnus X-1, as well as several other black hole candidates. The overall similarity of the source properties to those of other Galactic black holes classify XTE J1752-223 as a new stellar black hole candidate. We compare spectral and variability properties of XTE J1752-223 to Cygnus X-1 and discuss possible implications for various mechanisms of non-thermal emission.

  20. Bone-seeking radiopharmaceuticals for treatment of osseous metastases, Part 1: α therapy with 223Ra-dichloride.

    Science.gov (United States)

    Pandit-Taskar, Neeta; Larson, Steven M; Carrasquillo, Jorge A

    2014-02-01

    Metastatic disease to bone is commonly seen in the advanced stages of many cancers. The cardinal symptom, pain, is often the cause of significant morbidity and reduced quality of life. Treatment of bone pain includes nonsteroidal analgesics and opiates; however, long-term use of these drugs is commonly associated with significant side effects, and tolerance is common. External-beam radiation therapy is effective mainly in localized disease sites. Bone-targeting radiopharmaceuticals are beneficial in the management of patients with multiple metastatic lesions. This article focuses on the 3 most commonly used agents: the Food and Drug Administration-approved (89)Sr-chloride, (153)Sm-ethylenediaminetetramethylene phosphonic acid (EDTMP), and (223)Ra-dichloride. We will discuss the physical characteristics, clinical data, dosage, and administration of these agents, including optimal patient selection and toxicity associated with their use. These radioactive agents have proven efficacy in the treatment of painful osseous metastases from prostate cancer and breast cancer. Significant recent advances include use of these agents in combination with chemotherapy and the use of the α emitter (223)Ra-dichloride in prostate cancer, primarily to improve survival and skeletal related events. The review is presented in 2 parts. The first will discuss the characteristics and clinical use of (223)Ra-dichloride, and the second will discuss the β emitters (89)Sr and (153)Sm-EDTMP.

  1. Measurement of 223Ra and 224Ra via RaDeCC and its application at Liaohe River Estuary%辽河口水体中223Ra、224Ra的RaDeCC法测定及应用

    Institute of Scientific and Technical Information of China (English)

    许博超; 姚庆祯; 江雪艳; 于志刚

    2012-01-01

    介绍了同步延时计数法,并应用其对辽河口水体中的223 Ra和224 Ra进行了测定,结合pH、DO、COD、叶绿素、营养盐等参数对223 Ra和224 Ra的放射性活度及其比值随盐度的变化和影响因素进行了讨论,继而以“镭同位素表观年龄模型”计算了辽河口水体的停留时间.结果表明:2010年4月,辽河口调查水域水体中的224 Ra、223 Ra随盐度变化趋势不保守,在盐度为10和26的海域出现了放射性活度高峰值,可能分别由悬浮颗粒物解吸和滨岸咸化的地下水排放等因素造成.水体停留时间随着离开河口距离的增大而增大,最大值出现在盐度为14的海域,达到8.34 d.%Radium delayed coincidence countering system has become the state-of-the-art technique for short-lived radium isotopes (223Ra, 224Ra) measurement. In this paper, we described this system, and used it to measure 223Ra, 224Ra activities at the Liaohe River Estuary. Other parameters, like pH, DO, COD, petroleum hydrocarbon, chlorophyll and nutrients, were used to demonstrate the distribution and impact factors of isotopes activity and ratio versus salinity. Furthermore, the "Apparent Radium Age" model was applied to assess the water residence time at this area. Ra and Ra were not conservative with salinity at the studying area in A-pril, 2010. Two peak activities at salinity 10 and 26 were caused by suspended particles desorption and submarine groundwater discharge , respectively. Water residence time increased with the distance. The oldest age was 8. 34 days at the area with salinity of 14.

  2. Multidrug resistant 2009 A/H1N1 influenza clinical isolate with a neuraminidase I223R mutation retains its virulence and transmissibility in ferrets.

    Directory of Open Access Journals (Sweden)

    Erhard van der Vries

    2011-09-01

    Full Text Available Only two classes of antiviral drugs, neuraminidase inhibitors and adamantanes, are approved for prophylaxis and therapy against influenza virus infections. A major concern is that influenza virus becomes resistant to these antiviral drugs and spreads in the human population. The 2009 pandemic A/H1N1 influenza virus is naturally resistant to adamantanes. Recently a novel neuraminidase I223R mutation was identified in an A/H1N1 virus showing cross-resistance to the neuraminidase inhibitors oseltamivir, zanamivir and peramivir. However, the ability of this virus to cause disease and spread in the human population is unknown. Therefore, this clinical isolate (NL/2631-R223 was compared with a well-characterized reference virus (NL/602. In vitro experiments showed that NL/2631-I223R replicated as well as NL/602 in MDCK cells. In a ferret pathogenesis model, body weight loss was similar in animals inoculated with NL/2631-R223 or NL/602. In addition, pulmonary lesions were similar at day 4 post inoculation. However, at day 7 post inoculation, NL/2631-R223 caused milder pulmonary lesions and degree of alveolitis than NL/602. This indicated that the mutant virus was less pathogenic. Both NL/2631-R223 and a recombinant virus with a single I223R change (recNL/602-I223R, transmitted among ferrets by aerosols, despite observed attenuation of recNL/602-I223R in vitro. In conclusion, the I223R mutated virus isolate has comparable replicative ability and transmissibility, but lower pathogenicity than the reference virus based on these in vivo studies. This implies that the 2009 pandemic influenza A/H1N1 virus subtype with an isoleucine to arginine change at position 223 in the neuraminidase has the potential to spread in the human population. It is important to be vigilant for this mutation in influenza surveillance and to continue efforts to increase the arsenal of antiviral drugs to combat influenza.

  3. Sublethal exposure to alpha radiation (223Ra dichloride) enhances various carcinomas' sensitivity to lysis by antigen-specific cytotoxic T lymphocytes through calreticulin-mediated immunogenic modulation.

    Science.gov (United States)

    Malamas, Anthony S; Gameiro, Sofia R; Knudson, Karin M; Hodge, James W

    2016-12-27

    Radium-223 dichloride (Xofigo®; 223Ra) is an alpha-emitting radiopharmaceutical FDA-approved for the treatment of bone metastases in patients with advanced castration-resistant prostate cancer. It is also being examined clinically in patients with breast and lung carcinoma and patients with multiple myeloma. As with other forms of radiation, the aim of 223Ra is to reduce tumor burden by directly killing tumor cells. External beam (photon) and proton radiation have been shown to augment tumor sensitivity to antigen-specific CD8+ cytotoxic T lymphocytes (CTLs). However, little is known about whether treatment with 223Ra can also induce such immunogenic modulation in tumor cells that survive irradiation. We examined these effects in vitro by exposing human prostate, breast, and lung carcinoma cells to sublethal doses of 223Ra. 223Ra significantly enhanced T cell-mediated lysis of each tumor type by CD8+ CTLs specific for MUC-1, brachyury, and CEA tumor antigens. Immunofluorescence analysis revealed that the increase in CTL killing was accompanied by augmented protein expression of MHC-I and calreticulin in each tumor type, molecules that are essential for efficient antigen presentation. Enhanced tumor-cell lysis was facilitated by calreticulin surface translocation following 223Ra exposure. The phenotypic changes observed after treatment appear to be mediated by induction of the endoplasmic reticulum stress response pathway. By rendering tumor cells more susceptible to T cell-mediated lysis, 223Ra may potentially be effective in combination with various immunotherapies, particularly cancer vaccines that are designed to generate and expand patients' endogenous antigen-specific T-cell populations against specific tumor antigens.

  4. 29 CFR 2.23 - Procedure where a decision concerning a demand is not made prior to the time a response to the...

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Procedure where a decision concerning a demand is not made prior to the time a response to the demand is required. 2.23 Section 2.23 Labor Office of the Secretary... concerning a demand is not made prior to the time a response to the demand is required. If the response...

  5. MicroRNA-223 Promotes Type I Interferon Production in Antiviral Innate Immunity by Targeting Forkhead Box Protein O3 (FOXO3).

    Science.gov (United States)

    Chen, Luoquan; Song, Yinjing; He, Li; Wan, Xiaopeng; Lai, Lihua; Dai, Feng; Liu, Yang; Wang, Qingqing

    2016-07-08

    Effective recognition of viral infection and subsequent triggering of antiviral innate immune responses are essential for the host antiviral defense, which is tightly regulated by multiple regulators, including microRNAs. Previous reports have shown that some microRNAs are induced during virus infection and participate in the regulation of the innate antiviral response. However, whether the type I IFN response is regulated by miR-223 is still unknown. Here, we reported that vesicular stomatitis virus (VSV) infection induced significant up-regulation of miR-223 in murine macrophages. We observed that miR-223 overexpression up-regulated type I IFN expression levels in VSV-infected macrophages. We also demonstrated that miR-223 directly targets FOXO3 to regulate the type I IFN production. Furthermore, type I IFN, which is triggered by VSV infection, is responsible for the up-regulation of miR-223, thus forming a positive regulatory loop for type I IFN production. Our results uncovered a novel mechanism of miR-223-mediated regulation of type I IFN production in the antiviral innate immunity for the first time.

  6. Dosimetry of bone metastases in targeted radionuclide therapy with alpha-emitting {sup 223}Ra-dichloride

    Energy Technology Data Exchange (ETDEWEB)

    Pacilio, Massimiliano [Azienda Ospealiera San Camillo Forlianini, Rome (Italy). Dept. of Medical Physics; Ventroni, Guido; Mango, Lucio [Azienda Ospealiera San Camillo Forlianini, Rome (Italy). Dept. of Nuclear Medicin; De Vincentis, Giuseppe; Di Castro, Elisabetta; Frantellizzi, Viviana; Follacchio, Giulia Anna; Garkavaya, Tatiana [Rome Univ. (Italy). Dept. of Radiological, Oncological and Anatomo Pathological Sciences; Cassano, Bartolomeo; Lorenzon, Leda [Rome Univ. (Italy). Postgraduate School of Medical Physics; Pellegrini, Rosanna; Pani, Roberto [Rome Univ. (Italy). Dept. of Molecular Medicine; Ialongo, Pasquale [Azienda Ospealiera San Camillo Forlianini, Rome (Italy). Dept. of Radiology

    2016-01-15

    Ra-dichloride is an alpha-emitting radiopharmaceutical used in the treatment of bone metastases from castration-resistant prostate cancer. Image-based dosimetric studies remain challenging because the emitted photons are few. The aim of this study was to implement a methodology for in-vivo quantitative planar imaging, and to assess the absorbed dose to lesions using the MIRD approach. The study included nine Caucasian patients with 24 lesions (6 humeral head lesions, 4 iliac wing lesions, 2 scapular lesions, 5 trochanter lesions, 3 vertebral lesions, 3 glenoid lesions, 1 coxofemoral lesion). The treatment consisted of six injections (one every 4 weeks) of 50 kBq per kg body weight. Gamma-camera calibrations for {sup 223}Ra included measurements of sensitivity and transmission curves. Patients were statically imaged for 30 min, using an MEGP collimator, double-peak acquisition, and filtering to improve the image quality. Lesions were delineated on {sup 99m}Tc-MDP whole-body images, and the ROIs superimposed on the {sup 223}Ra images after image coregistration. The activity was quantified with background, attenuation, and scatter correction. Absorbed doses were assessed deriving the S values from the S factors for soft-tissue spheres of OLINDA/EXM, evaluating the lesion volumes by delineation on the CT images. In 12 lesions with a wash-in phase the biokinetics were assumed to be biexponential, and to be monoexponential in the remainder. The optimal timing for serial acquisitions was between 1 and 5 h, between 18 and 24 h, between 48 and 60 h, and between 7 and 15 days. The error in cumulated activity neglecting the wash-in phase was between 2 % and 12 %. The mean effective half-life (T{sub 1/2eff}) of {sup 223}Ra was 8.2 days (range 5.5-11.4 days). The absorbed dose (D) after the first injection was 0.7 Gy (range 0.2-1.9 Gy). Considering the relative biological effectiveness (RBE) of alpha particles (RBE = 5), D{sub RBE} = 899 mGy/MBq (range 340-2,450 mGy/MBq). The

  7. Radium-223 in the Treatment of Osteoblastic Metastases: A Critical Clinical Review

    Energy Technology Data Exchange (ETDEWEB)

    Humm, John L. [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, New York (United States); Sartor, Oliver [Departments of Medicine and Urology, Tulane Cancer Center, Tulane University School of Medicine, New Orleans, Louisiana (United States); Parker, Chris [Department of Clinical Oncology, Institute of Cancer Research, Sutton (United Kingdom); Bruland, Oyvind S. [Department of Oncology, Norwegian Radium Hospital and Institute for Clinical Medicine, University of Oslo, Oslo (Norway); Macklis, Roger, E-mail: rmmacklis@gmail.com [Department of Radiation Oncology, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio (United States)

    2015-04-01

    The element radium (Ra) was discovered by the Curies in 1898 and within a decade was in broad scientific testing for the management of several forms of cancer. The compound was known to give rise to a series of both high-energy particulate and penetrating γ-emissions. The latter found an important role in early 20th century brachytherapy applications, but the short-range α-particles seemed much less useful. Although highly cytotoxic when released within a few cell diameters of critical cell nuclei, the dense double-strand break damage was poorly repaired, and concerns regarding treatment-related toxicities and secondary malignancies halted clinical development. Moreover, the most common isotope of Ra has an exceptionally long half-life (>1600 years for {sup 226}Ra) that proved daunting when aiming for a systemic cancer therapy. Fortunately, other radium isotopes have more convenient half-lives while still producing cytotoxic α particles. Radium-223 dichloride has a half-life of 11.4 days, and this isotope was identified as an excellent candidate for radionuclide therapy of cancers metastatic to bone. The calcium-mimetic chemical properties of the radium allowed intravenous infusion with rapid uptake to sites of new bone formation. The highly efficient bone localization suggested a potential therapeutic role for osteoblastic bone metastases, and a series of phase 1, 2, and 3 clinical trials was undertaken to explore this possibility. This series of clinical explorations culminated in the ALSYMPCA trial, an international, placebo-controlled, phase 3 study that accrued 921 symptomatic men with bone-metastatic, castrate-resistant prostate cancer. Results of this trial demonstrated a prolongation of overall survival, and regulatory agencies around the world have now approved this product as a treatment for advanced prostate cancer.

  8. Natural history of spinal and bulbar muscular atrophy (SBMA): a study of 223 Japanese patients.

    Science.gov (United States)

    Atsuta, Naoki; Watanabe, Hirohisa; Ito, Mizuki; Banno, Haruhiko; Suzuki, Keisuke; Katsuno, Masahisa; Tanaka, Fumiaki; Tamakoshi, Akiko; Sobue, Gen

    2006-06-01

    Spinal and bulbar muscular atrophy (SBMA) is an adult-onset motoneuron disease caused by a CAG-repeat expansion in the androgen receptor (AR) gene and for which no curative therapy exists. However, since recent research may provide opportunities for medical treatment, information concerning the natural history of SBMA would be beneficial in planning future clinical trials. We investigated the natural course of SBMA as assessed by nine activities of daily living (ADL) milestones in 223 Japanese SBMA patients (mean age at data collection = 55.2 years; range = 30-87 years) followed from 1 to 20 years. All the patients were diagnosed by genetic analysis. Hand tremor was an early event that was noticed at a median age of 33 years. Muscular weakness occurred predominantly in the lower limbs, and was noticed at a median age of 44 years, followed by the requirement of a handrail to ascend stairs at 49, dysarthria at 50, dysphagia at 54, use of a cane at 59 and a wheelchair at 61 years. Twenty-one of the patients developed pneumonia at a median age of 62 and 15 of them died at a median age of 65 years. The most common cause of death in these cases was pneumonia and respiratory failure. The ages at onset of each ADL milestone were strongly correlated with the length of CAG repeats in the AR gene. However CAG-repeat length did not correlate with the time intervals between each ADL milestone, suggesting that although the onset age of each ADL milestone depends on the CAG-repeat length in the AR gene, the rate of disease progression does not. The levels of serum testosterone, an important triggering factor for polyglutamine-mediated motoneuron degeneration, were maintained at relatively high levels even at advanced ages. These results provide beneficial information for future clinical therapeutic trials, although further detailed prospective studies are also needed.

  9. miR-223 contributes to the AGE-promoted apoptosis via down-regulating insulin-like growth factor 1 receptor in osteoblasts.

    Science.gov (United States)

    Qin, Yi; Ye, Jichao; Wang, Peng; Gao, Liangbin; Wang, Suwei; Shen, Huiyong

    2016-01-01

    Advanced glycation end products (AGEs) have been confirmed to induce bone quality deterioration in diabetes mellitus (DM), and to associate with abnormal expression of miRNAs in DM patients or in vitro Recently, miRNAs have been recognized to mediate the onset or progression of DM. In the present study, we investigated the regulation on miR-223 level by AGE-BSA treatment in osteoblast-like MC3T3-E1 cells, with real-time quantitative PCR assay. And then we examined the inhibition of insulin-like growth factor 1 receptor (IGF-1R) expression by miR-223, via targeting of the 3' UTR of IGF-1R with real-time quantitative PCR, western blotting and luciferase reporter assay. Then we explored the regulation of miR-223 and IGF-1R levels, via the lentivirus-mediated miR-223 inhibition and IGF-1R overexpression in the AGE-BSA-induced apoptosis in MC3T3-E1 cells. It was demonstrated that AGE-BSA treatment with more than 100 μg/ml significantly up-regulated miR-223 level, whereas down-regulated IGF-1R level in MC3T3-E1 cells. And the up-regulated miR-223 down-regulated IGF-1R expression in both mRNA and protein levels, via targeting the 3' UTR of IGF-1R Moreover, though the AGE-BSA treatment promoted apoptosis in MC3T3-E1 cells, the IGF-1R overexpression or the miR-223 inhibition significantly attenuated the AGE-BSA-promoted apoptosis in MC3T3-E1 cells. In summary, our study recognized the promotion of miR-223 level by AGE-BSA treatment in osteoblast-like MC3T3-E1 cells. The promoted miR-223 targeted IGF-1R and mediated the AGE-BSA-induced apoptosis in MC3T3-E1 cells. It implies that miR-223 might be an effective therapeutic target to antagonize the AGE-induced damage to osteoblasts in DM.

  10. ANALYSIS OF PHYSICAL EXAMINATION RESULTS OF 223 ENTERPRISE OFFICERS IN CHENGDE CITY OF HEBEI PROVINCE%河北承德市某事业单位223名职工体检结果分析

    Institute of Scientific and Technical Information of China (English)

    冯增斌; 王振潮; 高宇; 贺晓磊

    2011-01-01

    [目的]了解和掌握某事业单位223名职工的疾病谱和身体健康状况.[方法]对近期在医院接受体检的某事业单位223名职工的检查结果进行统计分析.[结果]职工中位于前4位疾病是高血脂占43.50%,高血压占25.11%,脂肪肝占24.66%,高血糖占13%;男性职工高血脂、脂肪肝和肝功能异常患病率均高于女性,两者比较差异均有统计学意义(P<0.05);随年龄增长,多种常见疾病发生率均明显增加.[结论]定期检测,积极开展健康教育,采取生活方式干预或药物疗法以控制高血脂、高血压、高血糖和脂肪肝,减少心脑血管疾病的发生.%[Objective]To know the disease and physical conditions in 223 enterprise officers.[Methods]We selected the physical examination results of 223 enterprise officer for statistical analysis.[Results]The detection rates of hyperlipidemia, hypertension, fatty liver and hyperglycemia were 43.50%, 25.11%, 24.66%, and 13%.Hyperlipidemia, fatty liver, dysfunction of liver were significantly higher in the male workers than those in the female workers (P < 0.05).With the age increasing, the common disease incidence increased significantly.[Conclusion]Paying more attention to the healthy examination, reinforcing the health education and intervention, changing the Unhealthy lifestyle or selecting medicine therapy to control hyperlipidemia,, hypertension, fatty liver and hyperglycemia, will reduce cardiovascular and cerebrovascular diseases.

  11. Diapycnal nutrient fluxes across the benthic boundary layers at the continental slopes off Peru and Mauritania based on 224Ra/223Ra-ratios

    Science.gov (United States)

    Reichert, P.; Scholten, J. C.; Eisenhauer, A.; Miquel, J. C.; Gasser, B.; Liebetrau, V.; Sommer, S.; Dengler, M.; Bryant, L. D.

    2014-12-01

    Marine sediments may act as sources and/or sinks of nutrients in oxygen minimum zones (OMZs) where solute fluxes between sediments and the above water column are believed to be an important component for the bioproductivity in the mixed layer. However, solute fluxes between sediments and the water column above are difficult to obtain. We determined the diapycnal nutrient fluxes across the benthic boundary layer at the OMZs off Peru and Mauritania using nutrient concentration profiles and vertical diffusion coefficients derived from 224Ra/223Ra isotope ratios. The radium isotopes 223Ra (half-life: 11.4 days) and 224Ra (half-life: 3.66 days) are produced in sediments by radioactive decay of 227Th and 228Th ,respectively: Diffusion of radium out of the sediments results in gradients of 224Ra/223Ra above the sediments; and these gradients may be used to estimate vertical diffusion coefficients. We determined 224Ra/223Ra along two transects at the continental slopes off Peru (12° S) and off Mauritania (18° N) in water depths between 65m - 1000mand 53m - 1100m, respectively. For the 223Ra/224Ra sampling we used acrylic fibers/cartridges coated with MnO2. The Mn-fibers were attached to benthic landers (moored for ~ 24 hours at the sea floor) and the Mn-cartridges were placed in series on several in-situ filtration pumps (Challenger Oceanic and McLane pumps). 223Ra and 224Ra were measured on-board using a delayed coincidence counting system (RaDeCC). In our presentation we will present the 224Ra/223Ra distribution along these transects investigated and we will compare the diapycnal radium-derived nutrient fluxes with those determined using benthic chambers and microstructure measurements.

  12. The black hole candidate XTE J1752-223 towards and in quiescence: optical and simultaneous X-ray-radio observations

    Science.gov (United States)

    Ratti, E. M.; Jonker, P. G.; Miller-Jones, J. C. A.; Torres, M. A. P.; Homan, J.; Markoff, S.; Tomsick, J. A.; Kaaret, P.; Wijnands, R.; Gallo, E.; Özel, F.; Steeghs, D. T. H.; Fender, R. P.

    2012-07-01

    We present optical, X-ray and radio observations of the black hole transient (BHT) XTE J1752-223 towards and in quiescence. Optical photometry shows that the quiescent magnitude of XTE J1752-223 is fainter than 24.4 mag in the i' band. A comparison with measurements of the source during its 2009-2010 outburst shows that the outburst amplitude is more than 8 mag in the i' band. Known X-ray properties of the source combined with the faintness of the quiescence optical counterpart and the large outburst optical amplitude point towards a short orbital-period system (Porb≲ 6.8 h) with an M type (or later) mass donor, at a distance of 3.5 ≲d≲ 8 kpc. Simultaneous X-ray and radio data were collected with Chandra and the Expanded Very Large Array (EVLA), allowing constraints to be placed on the quiescent X-ray and radio flux of XTE J1752-223. Furthermore, using data covering the final stage of the outburst decay, we investigated the low-luminosity end of the X-ray-radio correlation for this source and compared it with other BHTs. We found that XTE J1752-223 adds to the number of outliers with respect to the 'standard' X-ray-radio luminosity relation. Furthermore, XTE J1752-223 is the second source, after the BHT H1743-322, that shows a transition from the region of the outliers towards the 'standard' correlation at low luminosity. Finally, we report on a faint, variable X-ray source we discovered with Chandra at an angular distance of ˜2.9 arcsec to XTE J1752-223 and at a position angle consistent with that of the radio jets previously observed from the BHT. We discuss the possibility that we detected X-ray emission associated with a jet from XTE J1752-223.

  13. Association of 8q22.3 locus in Chinese Han with idiopathic premature ovarian failure (POF).

    Science.gov (United States)

    Qin, Yingying; Zhao, Han; Xu, Jianfeng; Shi, Yongyong; Li, Zhiqiang; Qiao, Jie; Liu, Jiayin; Qin, Chunrong; Ren, Chune; Li, Jie; Chen, Shiling; Cao, Yunxia; Simpson, Joe Leigh; Chen, Zi-Jiang

    2012-01-15

    Premature ovarian failure (POF) is a complex heritable disorder known to be caused by chromosomal abnormalities and to date a limited number of known mutations, often autosomal. We sought to identify additional genetic loci associated with POF by performing the first large-scale genome-wide association study (GWAS). GWAS, using Affymetrix SNP 6.0 chip, was conducted in an initial discovery set of 391 well-documented (follicle-stimulating hormone >40 IU/ml) Chinese Han POF patients, compared with 895 unrelated Chinese female controls. A replication study on the most significant loci was then performed in an independent set of 400 cases and 800 controls. Suggestive significant associations were observed at 8q22.3. Replication of eight single-nucleotide polymorphisms (SNPs) (rs10464815, rs10808365, rs3847152, rs3847153, rs3847154, rs3843552, rs10955242, rs3843555) (P ≤ 3.86 × 10(-6)) was confirmed in verification sets. No specific candidate gene was found in the immediate region of 8q22.3. This GWAS, involving by far the largest sample of POF cases accumulated to date, revealed heretofore unrecognized association between POF and a novel genetic locus or region of unknown nature on 8q22.3. We speculate existence of a long-distance regulatory region that has relevance to the control of ovarian differentiation or oogenesis. Given failure to find association with any of the other autosomal regions known to harbor genes causing ovarian failure, our findings also underscore the likelihood of considerable genetic and etiologic heterogeneity in POF and the need for additional approaches like whole-genome sequencing.

  14. Superconductivity in 2-2-3 Y2Ba2Cu3O(sub 8+ delta)

    Science.gov (United States)

    Joshi, H. H.; Baldha, G. J.; Jotania, R. B.; Joshi, S. M.; Mohan, H.; Pandya, P. B.; Pandya, H. N.; Kulkarni, R. G.

    1990-01-01

    Researchers synthesized a new high T(sub c) 2-2-3 superconductor (Y2Ba2Cu3O8+delta) by a special preparation technique and characterized it by ac-susceptibility measurements. Diamagnetism and Meissner effect sets in at low fields and superconducting transition onsets at 90 K. The systematic investigation of the real and imaginary components of ac-susceptibility as a function of temperature and applied ac magnetic field reveals that the magnetic behavior is that of a granular type superconductor.

  15. A 47,X,+t(X;X)(p22.3;p22.3)del(X)(p11.23q11.2),Y Klinefelter variant with morbid obesity.

    Science.gov (United States)

    Kim, Youngsook; Kim, Won Jin; Huh, Ji Hye; Lee, Sujin; Kim, Daham; Hong, Jae Won; Lee, Eun Jig

    2013-03-01

    Klinefelter syndrome is the most common type of genetic cause of hypogonadism. This syndrome is characterized by the presence of 1 or more extra X chromosomes. Phenotype manifestations of this syndrome are small testes, fibrosis of the seminiferous tubules, inability to produce sperm, gynecomastia, tall stature, decrease of serum testosterone and increases of luteinizing hormone and follicle stimulating hormone. Most patients with Klinefelter syndrome are tall, with slender body compositions, and reports of obesity are rare. We report the case of a 35-yr-old man with hypogonadism and morbid obesity and diabetes mellitus. He had gynecomastia, small testes and penis, very sparse body hair and his body mass index was 44.85. He did not report experiencing broken voice and was able to have erections. We conducted a chromosome study. His genotype was 47,X,+t(X;X)(p22.3;p22.3)del(X)(p11.23q11.2). In this case, the patient was diagnosed as Klinefelter syndrome. He showed rare phenotypes like morbid obesity and average height and the phenotype may be caused by the karyotype and the excess number of X chromosome. Further studies of the relationship between chromosomes and phenotype are warranted.

  16. MAXI GSC observations of a spectral state transition in the black hole candidate XTE J1752-223

    CERN Document Server

    Nakahira, Satoshi; Sugizaki, Mutsumi; Ueda, Yoshihiro; Negoro, Hitoshi; Ebisawa, Ken; Kawai, Nobuyuki; Matsuoka, Masaru; Tsunemi, Hiroshi; Daikyuji, Arata; Eguchi, Satoshi; Hiroi, Kazuo; Ishikawa, Masaki; Ishiwata, Ryoji; Isobe, Naoki; Kawasaki, Kazuyoshi; Kimura, Masashi; Kohama, Mitsuhiro; Mihara, Tatehiro; Miyoshi, Sho; Morii, Mikio; Nakagawa, Yujin E; Nakajima, Motoki; Ozawa, Hiroshi; Sootome, Tetsuya; Sugimori, Kousuke; Suzuki, Motoko; Tomida, Hiroshi; Ueno, Shiro; Yamamoto, Takayuki; Yoshida, Atsumasa

    2010-01-01

    We present the first results on the black hole candidate XTE J1752-223 from the Gas Slit Camera (GSC) on-board the Monitor of All-sky X-ray Image (MAXI) on the International Space Station. Including the onset of the outburst reported by the Proportional Counter Array on-board the Rossi X-ray Timing Explorer on 2009 October 23, the MAXI/GSC has been monitoring this source approximately 10 times per day with a high sensitivity in the 2-20 keV band. XTE J1752-223 was initially in the low/hard state during the first 3 months. An anti-correlated behavior between the 2-4 keV and 4-20 keV bands were observed around January 20, 2010, indicating that the source exhibited the spectral transition to the high/soft state. A transient radio jet may have been ejected when the source was in the intermediate state where the spectrum was roughly explained by a power-law with a photon index of 2.5-3.0. The unusually long period in the initial low/hard state implies a slow variation in the mass accretion rate, and the dramatic s...

  17. Application of the Nonballistic Model to the Black Hole Candidate XTE J1752-223 and the Quasar NRAO 150

    Science.gov (United States)

    Zheng, T. Y.; Gong, B. P.

    2017-02-01

    Optical and radio observations of the black hole candidate XTE J1752-223 have exhibited a slightly curved motion of the jet components, which is associated with its radio light curve. In addition, observations of the quasar NRAO 150 have revealed a core–jet structure wobbling with a high angular speed. In this paper, the phenomena displayed in these two different sources are interpreted as the precession of a bent jet. In such a scenario, hot spots reproduced at different separations from the core precess on the same precession cone, in which different components correspond to different propagation times to the observer. By fitting the kinematics of the components of XTE J1752-223 and its light curve with a curved pattern of precession period 314 days, we find that the propagation time can make an earlier event appear later, and the jet axis can oscillate during its precession. Simulating the quasar NRAO 150 with the same scenario reveals that the knots at larger separation from the core precess at a slower speed than those closer in. A possible mechanism relating to the cooling time of a component is proposed. These three new results are of importance in understanding the physics underlying the curved jet as well as the activity of the central engine of different black hole systems.

  18. The CALYMHA survey: Lya luminosity function and global escape fraction of Lya photons at z=2.23

    CERN Document Server

    Sobral, David; Best, Philip; Stroe, Andra; Röttgering, Huub; Oteo, Iván; Smail, Ian; Morabito, Leah; Paulino-Afonso, Ana

    2016-01-01

    We present the CAlibrating LYMan-$\\alpha$ with H$\\alpha$ (CALYMHA) pilot survey and new results on Lyman-$\\alpha$ (Lya) selected galaxies at z~2. We use a custom-built Lya narrow-band filter at the Isaac Newton Telescope, designed to provide a matched volume coverage to the z=2.23 Ha HiZELS survey. Here we present the first results for the COSMOS and UDS fields. Our survey currently reaches a 3$\\sigma$ line flux limit of ~4x10$^{-17}$ erg/s/cm$^{2}$, and a Lya luminosity limit of ~10$^{42.3}$ erg/s. We find 188 Lya emitters over 7.3x10$^5$ Mpc$^{3}$, but also find significant numbers of other line emitting sources corresponding to HeII, CIII] and CIV emission lines. These sources are important contaminants, and we carefully remove them, unlike most previous studies. We find that the Lya luminosity function at z=2.23 is very well described by a Schechter function up to L~10$^{43}$ erg/s with L$^*=10^{42.59+-0.05}$ erg/s, $\\phi^*=10^{-3.09+-0.08}$ Mpc$^{-3}$ and $\\alpha$=-1.75+-0.15. Above L~10$^{43}$ erg/s the...

  19. Development of separation technology for the removal of radium-223 from decayed thorium-227 in drug formulations. Material screening and method development.

    Science.gov (United States)

    Frenvik, Janne Olsen; Kristensen, Solveig; Ryan, Olav B

    2016-08-01

    Targeted thorium conjugates are currently being investigated as a new class of alpha-radiopharmaceuticals. The natural decay of thorium-227 ((227)Th) results in the ingrowth of radium-223 ((223)Ra). Consideration must, therefore, be given to define acceptable limits of (223)Ra in the drug product at the time of dose administration. By effective sequestration of (223)Ra, we aim to improve the radiochemical purity and extend the effective user window of drug products containing (227)Th. (223)Ra is the first progeny of (227)Th and the only one with a long half-life (days). We have, therefore, focused on the removal of this specific species since the progenies of (223)Ra will have a very limited lifetime in the formulation once (223)Ra is removed. In this study, we investigated a multitude of materials for their ability to reduce the (223)Ra level by: (1) passive diffusion or (2) by cartridge filtration on gravity columns. In addition, we probe the compatibility of these materials in the presence of antibody trastuzumab to assess the level of protein binding and estimate the quenching of radiolysis by binding of radionuclides. A screening matrix of organic and inorganic materials was established, i.e. strontium and calcium alginate gel beads, distearoyl phosphatidylglycerol (DSPG) liposomes, ceramic hydroxyapatite, Zeolite UOP type 4A and cation exchange resins AG50W-X8 and SOURCE 30S. First, passive diffusional uptake of (223)Ra by suspended materials present in the formulation was measured as a decrease in sample radioactivity after separation. Second, selected materials were packed on gravity columns in order to evaluate the efficiency of column separation versus diffusional adsorption. The retention of (223)Ra and (227)Th were characterized by measuring the radioactivity in the eluate and on the columns. Finally, the compatibility between trastuzumab, as a selected model antibody, and suspensions of the binding materials was analyzed during storage of the drug

  20. 微小RNA-223及高迁移率族蛋白-1在脓毒症患儿中的表达及意义%Expression of plasma microRNA-223 and HMGB-1 in pediatric sepsis patients and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    刘彩丽; 卢灵莉; 梁桂林; 郭影霞; 董艳飞

    2015-01-01

    Objective To investigate the changes of plasma microRNA-223(miR-223) and HMGB-1 in pediatric sepsis patients.Methods There were 49 children with sepsis enrolled in the study (sepsis group),severe sepsis group (n=25) and general group (n=24). Meanwhile, 50 healthy children (normal control group) were selected as control group. The expression levels of plasma miR-223and HMGB-1 (high mobility group box 1) were detected. The predictive values of miR-223and HMGB-1 in plasma of children with sepsis were evaluated by receiver operatingcharacteristic (ROC) curve.Results The plasma miR-223 and HMGB-1 expression levels in severe sepsis group and general group were up-regulated compared with those in the normal control group (F=63.02, 76.32,P<0.05). The area under ROC curve of miR-223,HMGB-1 predicting sepsis were 0.904 (95%CI 0.821-0.998), 0.748 (95%CI: 0.625-0.903). There was positive correlation between miR-223 and HMGB-1 (r=3.532, P<0.05). Conclusions The expression levels of plasma miR-223 in children with sepsis are signiifcantly up-regulated, which can be used as early diagnostic markers to relfect the severity of inlfammation in some degree.%目的 观察脓毒症患儿血浆中微小RNA-223(miR-223)及血清高迁移率族蛋白-1(HMGB-1)的表达.方法 选取49例脓毒症患儿,其中一般脓毒症24例、严重脓毒症25例,同时选取50例健康儿童作对照组,检测及比较血浆中miR-223及血清HMGB-1的表达水平.结果 严重脓毒症组、一般脓毒症组及对照组miR-223、HMGB-1表达的差异有统计学意义(F=63.02、76.32,P<0.05).miR-223和HMGB-1预测脓毒症的受试者工作特征曲线(ROC)下面积分别为0.904(95%CI:0.821-0.998),0.748(95%CI:0.625-0.903).miR-223与HMGB-1呈正相关(r=3.532,P<0.05).结论 脓毒症患儿外周血中miR-223及HMGB-1表达水平升高,两者联合检测对早期诊断脓毒症具有一定的临床价值.

  1. Hydrogen bonding between the QB site ubisemiquinone and Ser-L223 in the bacterial reaction centre – a combined spectroscopic and computational perspective^

    Science.gov (United States)

    Martin, Erik; Baldansuren, Amgalanbaatar; Lin, Tzu-Jen; Samoilova, Rimma I.; Wraight, Colin A.; Dikanov, Sergei A.; O’Malley, Patrick J.

    2012-01-01

    In the QB site of the Rba. sphaeroides photosynthetic reaction centre the donation of a hydrogen bond from the hydroxyl group of Ser-L223 to the ubisemiquinone formed after the first flash is debatable. In this study we use a combination of spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations to comprehensively explore this topic. We show that ENDOR, ESEEM and HYSCORE spectroscopic differences between the mutant L223SA and the wild type sample (WT) are negligible, indicating only minor perturbations in the ubisemiquinone spin density for the mutant sample. Qualitatively this suggests that a strong hydrogen bond does not exist in the WT between the Ser-L223 hydroxyl group and the semiquinone O1 atom, as removal of this hydrogen bond in the mutant should cause a significant redistribution of spin density in the semiquinone. We show quantitatively, using QM/MM calculations, that a WT model in which the Ser-L223 hydroxyl group is rotated to prevent hydrogen bond formation with the O1 atom of the semiquinone predicts negligible change for the L223SA mutant. This, together with the better agreement between key QM/MM calculated and experimental hyperfine couplings for the non-hydrogen bonded model, leads us to conclude that no strong hydrogen bond is formed between the Ser-L223 hydroxyl group and the semiquinone O1 atom after the first flash. The implications of this finding for quinone reduction in photosynthetic reaction centres are discussed. PMID:23016832

  2. MiR-223-3p as a Novel MicroRNA Regulator of Expression of Voltage-Gated K+ Channel Kv4.2 in Acute Myocardial Infarction

    Directory of Open Access Journals (Sweden)

    Xue Liu

    2016-06-01

    Full Text Available Background/Aims: Acute myocardial infarction (AMI is a devastating cardiovascular disease with a high rate of morbidity and mortality, partly due to enhanced arrhythmogenicity. MicroRNAs (miRNAs have been shown to participate in the regulation of cardiac ion channels and the associated arrhythmias. The purpose of this study was to test our hypothesis that miR-223-3p contributes to the electrical disorders in AMI via modulating KCND2, the gene encoding voltage-gated channel Kv4.2 that carries transient outward K+ current Ito. Methods: AMI model was established in male Sprague-Dawley (SD rats by left anterior descending artery (LAD ligation. Evans blue and TTC staining was used to measure infarct area. Ito was recorded in isolated ventricular cardiomyocytes or cultured neonatal rat ventricular cells (NRVCs by whole-cell patch-clamp techniques. Western blot analysis was employed to detect the protein level of Kv4.2 and real-time RT-PCR to determine the transcript level of miR-223-3p. Luciferase assay was used to examine the interaction between miR-223-3p and KCND2 in cultured NRVCs. Results: Expression of miR-223-3p was remarkably upregulated in AMI relative to sham control rats. On the contrary, the protein level of Kv4.2 and Ito density were significantly decreased in AMI. Consistently, transfection of miR-223-3p mimic markedly reduced Kv4.2 protein level and Ito current in cultured NRVCs. Co-transfection of AMO-223-3p (an antisense inhibitor of miR-223-3p reversed the repressive effect of miR-223-3p. Luciferase assay showed that miR-223-3p, but not the negative control, substantially suppressed the luciferase activity, confirming the direct binding of miR-223-3p to the seed site within the KCND2 sequence. Finally, direct intramuscular injection of AMO-223-3p into the ischemic myocardium to knockdown endogenous miR-223-3p decreased the propensity of ischemic arrhythmias. Conclusions: Upregulation of miR-223-3p in AMI repressed the expression of

  3. Stathmin1 plays oncogenic role and is a target of microRNA-223 in gastric cancer.

    Directory of Open Access Journals (Sweden)

    Wei Kang

    Full Text Available Stathmin1 (STMN1 is a candidate oncoprotein and prognosis marker in several kinds of cancers. This study was aimed to analyze its expression and biological functions in gastric cancer. The expression of STMN1 was evaluated by qRT-PCR, western blot and immunohistochemistry. The biological function of STMN1 was determined by MTT proliferation assays, monolayer colony formation and cell invasion assays using small interference RNA technique in gastric cancer cell lines. We also explored the regulation of STMN1 expression by microRNA-223. STMN1 was upregulated in gastric cancer cell lines and primary gastric adenocarcinomas. STMN1-positive tumors were more likely to be found in old age group and associated with p53 nuclear expression. In diffuse type gastric adenocarcinomas, STMN1 expression was correlated with age (p = 0.043, T stage (p = 0.004 and lymph node metastasis (p = 0.046. Expression of STMN1 in diffuse type gastric adenocarcinoma was associated with poor disease specific survival by univariate analysis (p = 0.01. STMN1 knockdown in AGS and MKN7 cell lines suppressed proliferation (p<0.001, reduced monolayer colony formation (p<0.001, inhibited cell invasion and migration ability (p<0.001 and induced G1 phase arrest. siSTMN1 could also suppress cell growth in vivo (p<0. 01. We finally confirmed that STMN1 is a putative downstream target of miR-223 in gastric cancer. Our findings supported an oncogenic role of STMN1 in gastric cancer. STMN1 might serve as a prognostic marker and a potential therapeutic target for gastric cancer.

  4. XTE J1752-223 in outburst: a persistent radio jet, dramatic flaring, multiple ejections and linear polarization

    Science.gov (United States)

    Brocksopp, C.; Corbel, S.; Tzioumis, A.; Broderick, J. W.; Rodriguez, J.; Yang, J.; Fender, R. P.; Paragi, Z.

    2013-06-01

    The black hole candidate, XTE J1752-223, was discovered in 2009 October when it entered an outburst. We obtained radio data from the Australia Telescope Compact Array for the duration of the ˜9 month event. The light curves show that the radio emission from the compact jet persisted for the duration of an extended hard state and through the transition to the intermediate state. The flux then rose rapidly by a factor of 10 and the radio source entered a series of at least seven maxima, the first of which was likely to be emission associated with the compact jet. The subsequent six flares were accompanied by variable behaviour in terms of radio spectrum, degree of linear polarization, morphology and associated X-ray behaviour. They were, however, remarkably similar in terms of the estimated minimum power required to launch such an ejection event. We compare the timing of radio peaks with the location of the ejecta, imaged by contemporaneous Very Long Baseline Interferometry experiments. We then discuss the mechanism behind the events, in terms of whether discrete ejections are the most likely description of the behaviour. One ejection, at least, appears to be travelling with apparent superluminal motion. The range of properties, however, suggests that multiple mechanisms may be relevant and that at least some of the emission is coming from shocked interactions amongst the ejecta and between the ejecta and the interstellar medium. We also compare the radio flux density with the X-ray source during the hard state and conclude that XTE J1752-223 is a radio-weak/X-ray-bright outlier on the universal correlation for black hole transient sources.

  5. Changes in skeletal tumor activity on {sup 18}F-choline PET/CT in patients receiving {sup 223}radium radionuclide therapy for metastatic prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Miyazaki, Kyle S. [Oncology Research Dept. and Hamamatsu/Queen' s PET Imaging Center, The Queen' s Medical Center, Honolulu (United States); Kang, Yu; Kwee, Sandi A. [Dept. of Medical Physics, School of Allied Health Sciences, University of Nevada Las Vegas, Las Vegas (United States)

    2015-06-15

    Radium-223 dichloride is an alpha-emitting radiopharmaceutical shown to prolong survival in patients with castrate-resistant prostate cancer (CRPC) and symptomatic skeletal metastases. This report describes in two patients the acute changes in bone metastatic activity detected by F-18 choline PET/CT imaging midway during treatment with radium-223 dichloride. In addition to visual and standardized uptake value analysis, changes in the whole-body tumor burden were quantified by measuring the difference in net metabolically active tumor volume (MATV) and total lesion activity (TLA) between pre- and mid-treatment PET scans. After the third dose of radium-223 dichloride, near-total disappearance of abnormal skeletal activity was observed in one case (net MATV change from 260.7 to 0.8 cc; net TLA change from 510.7 to 2.1), while a heterogeneous tumor response was observed in the other (net MATV change from 272.2 to 241.3 cc; net TLA change from 987.1 to 779.4). Corresponding normalization and persistent elevation in serum alkaline phosphatase levels were observed in these cases, respectively. Further research is needed to determine the predictive value of serial F-18 choline PET/CT imaging in patients receiving radium-223 dichloride for CRPC.

  6. Association of LEP G2548A and LEPR Q223R Polymorphisms with Cancer Susceptibility: Evidence from a Meta-Analysis

    NARCIS (Netherlands)

    J. He (Jing); B. Xi (Bao); R. Ruiter (Rikje); T.-Y. Shi (Ting-Yan); M.-L. Zhu (Mei-Ling); M.-Y. Wang (Meng-Yun); Q.-X. Li (Qiao-Xin); X.-Y. Zhou (Xiao-Yan); L.-X. Qiu (Li-Xin); Q.-Y. Wei (Qing-Yi)

    2013-01-01

    markdownabstract__Background:__ Numerous epidemiological studies have examined associations of genetic variations in LEP (G2548A, -2548 nucleotide upstream of the ATG start site) and LEPR (Q223R, nonsynonymous SNP in exon 6) with cancer susceptibility; however, the findings are inconsistent. Therefo

  7. Dysbindin (DTNBP1, 6p22.3) is Associated with Childhood-Onset Psychosis and Endophenotypes Measured by the Premorbid Adjustment Scale (PAS)

    Science.gov (United States)

    Gornick, M. C.; Addington, A. M.; Sporn, A.; Gogtay, N.; Greenstein, D.; Lenane, M.; Gochman, P.; Ordonez, A.; Balkissoon, R.; Vakkalanka, R.; Weinberger, D. R.; Rapoport, J. L.; Straub, R. E.

    2005-01-01

    Straub "et al." ("2002") recently identified the 6p22.3 gene dysbindin (DTNBP1) through positional cloning as a schizophrenia susceptibility gene. We studied a rare cohort of 102 children with onset of psychosis before age 13. Standardized ratings of early development, medication response, neuropsychological and cognitive performance, premorbid…

  8. Metabolism of 2,2'-dihydroxybiphenyl by Pseudomonas sp. strain HBP1 : production and consumption of 2,2',3-trihydroxybiphenyl

    NARCIS (Netherlands)

    Kohler, Hans-Peter E.; Schmid, Andreas; Maarel, Marc van der

    1993-01-01

    Cells of Pseudomonas sp. strain HBP1 grown on 2-hydroxy- or 2,2'-dihydroxybiphenyl contain NADH-dependent monooxygenase activity that hydroxylates 2,2'-dihydroxybiphenyl. The product of this reaction was identified as 2,2',3-trihydroxybiphenyl by 1H nuclear magnetic resonance and mass spectrometry.

  9. Radium-223-Dichloride in Castration Resistant Metastatic Prostate Cancer—Preliminary Results of the Response Evaluation Using F-18-Fluoride PET/CT

    Directory of Open Access Journals (Sweden)

    Kalevi Kairemo

    2015-10-01

    Full Text Available The purpose of this study was to evaluate the outcome after Radium-223-dichloride (223RaCl2 treatment of patients with skeletal metastases of castration resistant prostate cancer using whole-body 18F-Fluoride PET/CT. Sodium 18F-fluoride [18F]-NaF PET/CT was performed prior the treatment of 223RaCl2, after the first cycle and after the sixth cycle. The skeletal metastases were analyzed quantitatively using modified PET response evaluation PERCIST criteria. The patients were also analyzed for S-PSA. All ten patients responded in [18F]-NaF scans after 6 cycles, but interim analysis after the 1st cycle did not give additional information about the outcome. The S-PSA decrease correlated with [18F]-NaF response, only 1 patient demonstrated progressive disease, i.e., >25% increase in S-PSA values during 223RaCl2. Our results (although preliminary suggest that 18F-Fluoride PET/CT is useful in the follow-up of castration resistant prostate cancer with skeletal metastases.

  10. miRNA-197 and miRNA-223 Predict Cardiovascular Death in a Cohort of Patients with Symptomatic Coronary Artery Disease.

    Directory of Open Access Journals (Sweden)

    Christian Schulte

    Full Text Available Circulating microRNAs (miRNAs have been described as potential diagnostic biomarkers in cardiovascular disease and in particular, coronary artery disease (CAD. Few studies were undertaken to perform analyses with regard to risk stratification of future cardiovascular events. miR-126, miR-197 and miR-223 are involved in endovascular inflammation and platelet activation and have been described as biomarkers in the diagnosis of CAD. They were identified in a prospective study in relation to future myocardial infarction.The aim of our study was to further evaluate the prognostic value of these miRNAs in a large prospective cohort of patients with documented CAD.Levels of miR-126, miR-197 and miR-223 were evaluated in serum samples of 873 CAD patients with respect to the endpoint cardiovascular death. miRNA quantification was performed using real time polymerase chain reaction (RT-qPCR.The median follow-up period was 4 years (IQR 2.78-5.04. The median age of all patients was 64 years (IQR 57-69 with 80.2% males. 38.9% of the patients presented with acute coronary syndrome (ACS, 61.1% were diagnosed with stable angina pectoris (SAP. Elevated levels of miRNA-197 and miRNA-223 reliably predicted future cardiovascular death in the overall group (miRNA-197: hazard ratio (HR 1.77 per one standard deviation (SD increase (95% confidence interval (CI 1.20; 2.60, p = 0.004, C-index 0.78; miRNA-223: HR 2.23 per one SD increase (1.20; 4.14, p = 0.011, C-index 0.80. In ACS patients the prognostic power of both miRNAs was even higher (miRNA-197: HR 2.24 per one SD increase (1.25; 4.01, p = 0.006, C-index 0.89; miRA-223: HR 4.94 per one SD increase (1.42; 17.20, p = 0.012, C-index 0.89.Serum-derived circulating miRNA-197 and miRNA-223 were identified as predictors for cardiovascular death in a large patient cohort with CAD. These results reinforce the assumption that circulating miRNAs are promising biomarkers with prognostic value with respect to future

  11. An N-terminally truncated envelope protein encoded by a human endogenous retrovirus W locus on chromosome Xq22.3

    Directory of Open Access Journals (Sweden)

    Roebke Christina

    2010-08-01

    Full Text Available Abstract Background We previously showed that the envelope (env sequence of a human endogenous retrovirus (HERV-W locus on chromosome Xq22.3 is transcribed in human peripheral blood mononuclear cells. The env open reading frame (ORF of this locus is interrupted by a premature stop at codon 39, but otherwise harbors a long ORF for an N-terminally truncated 475 amino acid Env protein, starting at an in-frame ATG at codon 68. We set out to characterize the protein encoded by that ORF. Results Transient expression of the 475 amino acid Xq22.3 HERV-W env ORF produced an N-terminally truncated HERV-W Env protein, as detected by the monoclonal anti-HERV-W Env antibodies 6A2B2 and 13H5A5. Remarkably, reversion of the stop at codon 39 in Xq22.3 HERV-W env reconstituted a full-length HERV-W Xq22.3 Env protein. Similar to the full-length HERV-W Env protein Syncytin-1, reconstituted full-length Xq22.3 HERV-W Env is glycosylated, forms oligomers, and is expressed at the cell surface. In contrast, Xq22.3 HERV-W Env is unglycosylated, does not form oligomers, and is located intracellularly, probably due to lack of a signal peptide. Finally, we reconfirm by immunohistochemistry that monoclonal antibody 6A2B2 detects an antigen expressed in placenta and multiple sclerosis brain lesions. Conclusions A partially defective HERV-W env gene located on chromosome Xq22.3, which we propose to designate ERVWE2, has retained coding capacity and can produce ex vivo an N-terminally truncated Env protein, named N-Trenv. Detection of an antigen by 6A2B2 in placenta and multiple sclerosis lesions opens the possibility that N-Trenv could be expressed in vivo. More generally, our findings are compatible with the idea that defective HERV elements may be capable of producing incomplete HERV proteins that, speculatively, may exert functions in human physiology or pathology.

  12. SU-E-T-588: Optimization of Imaging Following 223Ra Administration in Targeted Alpha-Emitting Radionuclide Therapy of Bone Metastases

    Energy Technology Data Exchange (ETDEWEB)

    Benabdallah, N; Bernardini, M [Hopital Europeen George Pompidou, Paris, Ile de France (France); Desbree, A [Institut de Radioprotection et de Surete Nucleaire, Fontenay-aux-roses, Ile-de-France (France); Labriolle-Vaylet, C de [Hopital Trousseau, Paris, Ile de France (France); Franck, D [Institut de Radioprotection et de Suretu Nucleaire, Fontenay Aux Roses, Ile de France (France)

    2015-06-15

    Purpose: With a growing demand of alpha-emitting radiopharmaceuticals, especially Xofigo ({sup 223}RaCl{sub 2}) which is used in the treatment of metastatic bone disease, the optimization of dosimetry becomes necessary. Indeed, in Europe, as stated on the council directive 2013/59/euratom, exposures of target volumes for radiotherapeutic purposes shall be individually planned taking into account that doses to non-target volumes and tissues shall be as low as reasonably achievable. To that aim, the possibility of imaging {sup 223}Ra was first investigated. Methods: The experiments were conducted at the Hopital Europeen Georges Pompidou with an Infinia Hawkeye 4 gamma camera, equipped with a medium-energy collimator. Imaging parameters, such as sensibility, spatial resolution and energy spectrum, were determined using several physical phantoms with a source of 6 MBq of {sup 223}Ra. Bone metastases were modeled with a NEMA Body Phantom to investigate image degradation based on the concentration of {sup 223}Ra. Results: The acquired energy spectrum allowed to visualize several photon peaks: at 85, 154 and 270 keV. Camera sensitivity measured from the phantom study was 102.3 cps/MBq for the 85 keV ± 20 %, 89.9 cps/MBq for the 154 ± 20 % window and 65.4 cps/MBq for the 270 ± 10 % window. The spatial resolution (full-width at half-maximum) was respectively 1.7, 1.9 and 1.8 cm for the three energy windows. SPECT/CT images of NEMA Body Phantom without and with attenuation have permitted to determine the best reconstruction parameters. Conclusion: This study has demonstrated that it is possible to obtain clinically relevant information from images of {sup 223}Ra. All these results will be valuable to analyze biodistribution imaging of the radiopharmaceutical in the patient body and go further in the reconstruction of patient images in order to personalize the dosimetry.

  13. Effects of the novel compound DK223 ([1E,2E-1,2-Bis(6-methoxy-2H-chromen-3-yl)methylene]hydrazine) on migration and proliferation of human keratinocytes and primary dermal fibroblasts.

    Science.gov (United States)

    Ho, Manh Tin; Kang, Hyun Sik; Huh, Jung Sik; Kim, Young Mee; Lim, Yoongho; Cho, Moonjae

    2014-07-23

    Wound healing plays an important role in protecting the human body from external infection. Cell migration and proliferation of keratinocytes and dermal fibroblasts are essential for proper wound healing. Recently, several studies have demonstrated that secondary compounds produced in plants could affect skin cells migration and proliferation. In this study, we identified a novel compound DK223 ([1E,2E-1,2-bis(6-methoxy-2H-chromen-3-yl)methylene]hydrazine) that concomitantly induced human keratinocyte migration and dermal fibroblast proliferation. We evaluated the regulation of epithelial and mesenchymal protein markers, such as E-cadherin and Vimentin, in human keratinocytes, as well as extracellular matrix (ECM) secretion and metalloproteinase families in dermal fibroblasts. DK223 upregulated keratinocyte migration and significantly increased the epithelial marker E-cadherin in a time-dependent manner. We also found that reactive oxygen species (ROS) increased significantly in keratinocytes after 2 h of DK223 exposure, returning to normal levels after 24 h, which indicated that DK223 had an early shock effect on ROS production. DK223 also stimulated fibroblast proliferation, and induced significant secretion of ECM proteins, such as collagen I, III, and fibronectin. In dermal fibroblasts, DK223 treatment induced TGF-β1, which is involved in a signaling pathway that mediates proliferation. In conclusion, DK223 simultaneously induced both keratinocyte migration via ROS production and fibroblast proliferation via TGF-β1 induction.

  14. HH 223: a parsec-scale H2 outflow in the star-forming region L723

    Science.gov (United States)

    López, R.; Acosta-Pulido, J. A.; Gómez, G.; Estalella, R.; Carrasco-González, C.

    2010-11-01

    Context. The dark cloud Lynds 723 (L723) is a low-mass star-forming region where one of the few known cases of a quadrupolar CO outflow has been reported. Two recent works have found that the radio continuum source VLA 2, towards the centre of the CO outflow, is actually a multiple system of young stellar objects (YSOs). Several line-emission nebulae that lie projected on the east-west CO outflow were detected in narrow-band Hα and [S ii] images. The spectra of the knots are characteristic of shock-excited gas (Herbig-Haro spectra), with supersonic blueshifted velocities, which suggests an optical outflow also powered by the VLA 2 YSO system of L723. Aims: Our aim is to study L723 in the near-infrared and look for line-emission nebulae associated with the optical and CO outflows. Methods: We imaged a field of ~5' × 5' centred on HH 223, which includes the whole region of the quadrupolar CO outflow with narrow-band filters centred on the [Fe ii] 1.644 μm and H2 2.122 μm lines, together with off-line Hc and Kc filters. The [Fe ii] and H2 line-emission structures were identified after extracting the continuum contribution, if any. Their positions were determined from an accurate astrometry of the images. Results: The H2 line-emission structures appear distributed over a region of 5.5 arcmin (~0.5 pc for a distance of 300 pc) at both sides of the VLA 2 YSO system, with an S-shape morphology, and are projected onto the east-west CO outflow. Most of them were resolved in smaller knotty substructures. The [Fe ii] emission only appears associated with HH 223. An additional nebular emission from the continuum in Hc and Kc appears associated with HH 223-K1, the structure closest to the VLA 2 YSO system, and could be tracing the cavity walls. Conclusions: We propose that the H2 structures form part of a large-scale near-infrared outflow, which is also associated with the VLA 2 YSO system. The current data do not allow us to discern which of the YSOs of VLA 2 is powering

  15. Analysis on the drug-resistance and distribution of 223 strains ofEscherichia coli%223株大肠埃希菌的耐药性分析及分布

    Institute of Scientific and Technical Information of China (English)

    冯莉

    2014-01-01

    Objective To investigate the drug-resistance and distribution ofEscherichia coli(E. coli) isolated in clinic, so as to provide evidence for the clinicians to use antibiotics on the basis of experience. Methods Totla of 223 strains ofE. coli were isolated from January 2011 to October 2011 in our hospital. Drug resistance of strains to the antibacterial drug that commonly used in clinical and the distribution were analyzed, respectively. Results The drug-resistance rates of 223 strains ofE. coli to imipenem, piperacillin/tazobactam, amikacin, cefoxitin, amoxicillin/clavulanic acid, ceftazidime, tobramycin, levofloxacin, cefepime, cefotaxime, aztreonam, gentamicin, ciprolfoxacin, cefazolin, ampicillin and cotrimoxazole were 0.45% (1 strain), 4.04% (9 strains), 9.42% (21 strains), 13.00% (29 strains), 14.80% (33 strains), 39.46% (88 strains), 41.70% (93 strains), 48.43 % (123 strains), 51.12% (114 strains), 54.71% (122 strains), 55.16% (123 strains), 55.16% (123 strains), 57.40% (128 strains), 62.78% (140 strains), 71.75% (160 strains) and 86.10%(192 strains), respectively. There were 40.81% (91 strains) ofE. coli were collected from urine specimens, 30.94% (69 strains) from sputum specimens and 9.87% (22 strains) from blood specimens.ConclusionsE. coliseparated in our hospital mainly caused urinary tract infections and respiratory tract infections. The drug-resistance rates to the antimicrobial drugs that commonly used in clinical varied widely and clinicians should use antibiotics on the basis of the susceptibility results.%目的:了解临床分离的大肠埃希菌的耐药性及分布,为临床经验用药提供理论依据。方法对2011年1月至2012年10月本院临床分离的223株大肠埃希菌对临床常用抗菌药物的耐药性及分布进行分析。结果223株大肠埃希菌对亚胺培南、哌拉西林/他唑巴坦、阿米卡星、头孢西丁、阿莫西林/棒酸、头孢他啶、妥布霉素、左氧氟沙星、头孢吡肟、头孢噻

  16. The CALYMHA survey: Ly$\\alpha$ escape fraction and its dependence on galaxy properties at $z=2.23$

    CERN Document Server

    Matthee, Jorryt; Oteo, Ivàn; Best, Philip; Smail, Ian; Röttgering, Huub; Paulino-Afonso, Ana

    2016-01-01

    We present the first results from our CAlibrating LYMan-$\\alpha$ with H$\\alpha$ (CALYMHA) pilot survey at the Isaac Newton Telescope. We measure Ly$\\alpha$ emission for 488 H$\\alpha$ selected galaxies at $z=2.23$ from HiZELS in the COSMOS and UDS fields with a specially designed narrow-band filter ($\\lambda_c$ = 3918 {\\AA}, $\\Delta\\lambda$= 52 {\\AA}). We find 17 dual H$\\alpha$-Ly$\\alpha$ emitters ($f_{\\rm Ly\\alpha} >5\\times10^{-17}$ erg s$^{-1}$ cm$^{-2}$, of which 5 are X-ray AGN). For star-forming galaxies, we find a range of Ly$\\alpha$ escape fractions (f$_{\\rm esc}$, measured with 3$"$ apertures) from $2$\\%$-30$\\%. These galaxies have masses from $3\\times10^8$ M$_{\\odot}$ to 10$^{11}$ M$_{\\odot}$ and dust attenuations E$(B-V)=0-0.5$. Using stacking, we measure a median escape fraction of $1.6\\pm0.5$\\% ($4.0\\pm1.0$\\% without correcting H$\\alpha$ for dust), but show that this depends on galaxy properties. The stacked f$_{\\rm esc}$ tends to decrease with increasing SFR and dust attenuation. However, at the h...

  17. Development and physical analysis of YAC contigs covering 7 Mb of Xp22.3-p22.2

    Energy Technology Data Exchange (ETDEWEB)

    Herrell, S.; Novo, F.J.; Charlton, R. [Univ. of Cambridge (United Kingdom)] [and others

    1995-01-20

    A total of YAC clones have been isolated from the region of Xp22.2-p22.3 extending from the amelogenin gene locus to DXS31. Restriction analysis of these clones in association with STS contenting and end clone analysis has facilitated the construction of 6 contigs covering a total of 7 Mb in which 20 potential CpG islands have been located. Thirty new STSs have been developed from probe and YAC end clone sequences, and these have been used in the analysis of patients suffering from different combinations of chondrodysplasia punctata, mental retardation, X-linked ichthyosis, and Kallmann syndrome. The results suggest that (1) the gene for chondrodysplasia punctata must lie between the X chromosome pseudoautosomal boundary (PABX) and DXS1145; (2) a gene for mental retardation lies between DXS1145 and the sequence tagged site GS1; and (3) the gene for ocular albinism type 1 lies proximal to the STS G13. The CpG islands within the YAC contigs constitute valuable markers for the potential positions of genes. Genes found associated with any of these potential CpG islands would be possible candidates for the disease genes mentioned above. 47 refs., 3 figs., 5 tabs.

  18. XTE J1752-223 in outburst: a persistent radio jet, dramatic flaring, multiple ejections and linear polarisation

    CERN Document Server

    Brocksopp, Catherine; Tzioumis, Tasso; Broderick, Jess; Rodriguez, Jerome; Yang, Jun; Fender, Rob; Paragi, Zsolt

    2013-01-01

    The black hole candidate, XTE J1752-223, was discovered in 2009 October when it entered an outburst. We obtained radio data from the Australia Telescope Compact Array for the duration of the ~9 month event. The lightcurves show that the radio emission from the compact jet persisted for the duration of an extended hard state and through the transition to the intermediate state. The flux then rose rapidly by a factor of 10 and the radio source entered a series of at least 7 maxima, the first of which was likely to be emission associated with the compact jet. The subsequent 6 flares were accompanied by variable behaviour in terms of radio spectrum, degree of linear polarisation, morphology and associated X-ray behaviour. They were, however, remarkably similar in terms of the estimated minimum power required to launch such an ejection event. We compare the timing of radio peaks with the location of the ejecta, imaged by contemporaneous VLBI experiments. We then discuss the mechanism behind the events, in terms of...

  19. A decelerating jet observed by the EVN and VLBA in the X-ray transient XTE J1752-223

    CERN Document Server

    Yang, J; Corbel, S; Paragi, Z; Tzioumis, T; Fender, R P

    2010-01-01

    The recently discovered Galactic X-ray transient XTE J1752-223 entered its first known outburst in 2010, emitting from the X-ray to the radio regimes. Its general X-ray properties were consistent with those of a black hole candidate in various spectral states, when ejection of jet components is expected. To verify this, we carried out very long baseline interferometry (VLBI) observations. The measurements were carried out with the European VLBI Network (EVN) and the Very Long Baseline Array (VLBA) at four epochs in 2010 February. The images at the first three epochs show a moving jet component that is significantly decelerated by the last epoch, when a new jet component appears that is likely to be associated with the receding jet side. The overall picture is consistent with an initially mildly relativistic jet, interacting with the interstellar medium or with swept-up material along the jet. The brightening of the receding ejecta at the final epoch can be well explained by initial Doppler deboosting of the e...

  20. Multiwavelength observations of the black hole transient XTE J1752-223 during its 2010 outburst decay

    CERN Document Server

    Chun, Y Y; Kalemci, E; Güver, T; Tomsick, J A; Buxton, M M; Brocksopp, C; Corbel, S; Cabrera-Lavers, A

    2013-01-01

    Galactic black hole transients show many interesting phenomena during outburst decays. We present simultaneous X-ray (\\emph{RXTE}, \\emph{Swift}, and \\emph{INTEGRAL}), and optical/near-infrared (O/NIR) observations (SMARTS) of the X-ray transient XTE J1752$-$223 during its outburst decay in 2010. The multiwavelength observations over 150 days in 2010 cover the transition from soft to hard spectral state. We discuss the evolution of radio emission is with respect to the O/NIR light curve which shows several flares. One of those flares is bright and long, starting about 60 days after the transition in X-ray timing properties. During this flare, the radio spectral index becomes harder. Other smaller flares occur along with the X-ray timing transition, and also right after the detection of the radio core. We discuss the significances of these flares. Furthermore, using the simultaneous broadband X-ray spectra including \\emph{INTEGRAL}, we find that a high energy cut-off with a folding energy near 250 keV is necess...

  1. A late jet rebrightening revealed from multi-wavelength monitoring of the black hole candidate XTE J1752-223

    CERN Document Server

    Russell, D M; Muñoz-Darias, T; Lewis, F; Motta, S; Stiele, H; Belloni, T; Miller-Jones, J C A; Jonker, P G; O'Brien, K; Homan, J; Casella, P; Gandhi, P; Soleri, P; Markoff, S; Maitra, D; Gallo, E; Bel, M Cadolle

    2011-01-01

    We present optical monitoring of the black hole candidate XTE J1752-223 during its 2009 - 2010 outburst and decay to quiescence. The optical light curve can be described by an exponential decay followed by a plateau, then a more rapid fade towards quiescence. The plateau appears to be due to an extra component of optical emission that brightens and then fades over ~ 40 days. We show evidence for the origin of this optical 'flare' to be the synchrotron jet during the decaying hard state, and we identify and isolate both disc and jet components in the spectral energy distributions. The optical flare has the same morphology and amplitude as a contemporaneous X-ray rebrightening. This suggests a common origin, but no firm conclusions can be made favouring or disfavouring the jet producing the X-ray flare. The quiescent optical magnitudes are B >= 20.6, V >= 21.1, R >= 19.5, i' >= 19.2. From the optical outburst amplitude we estimate a likely orbital period of < 22 h. We also present near-infrared (NIR) photome...

  2. HH 223: a parsec-scale H2 outflow in the star-forming region L723

    CERN Document Server

    Lopez, R; Gomez, G; Estalella, R; Carrasco-Gonzalez, C

    2010-01-01

    The dark cloud Lynds 723 (L723) is a low-mass star-forming region where one of the few known cases of a quadrupolar CO outflow has been reported. Two recent works have found that the radio continuum source VLA 2, towards the centre of the CO outflow, is actually a multiple system of young stellar objects (YSOs). Several line-emission nebulae that lie projected on the east-west CO outflow were detected in narrow-band Halpha and [SII] images. The spectra of the knots are characteristic of shock-excited gas (Herbig-Haro spectra), with supersonic blueshifted velocities, which suggests an optical outflow also powered by the VLA 2 YSO system of L723. We imaged a field of ~5' X 5' centred on HH 223, which includes the whole region of the quadrupolar CO outflow with nir narrow-band filters . The H2 line-emission structures appear distributed over a region of 5.5' (0.5 pc for a distance of 300 pc) at both sides of the VLA 2 YSO system, with an S-shape morphology, and are projected onto the east-west CO outflow. Most o...

  3. Multiwavelength Observations of the Black Hole Transient XTE J1752-223 during Its 2010 Outburst Decay

    Science.gov (United States)

    Chun, Y. Y.; Dinçer, T.; Kalemci, E.; Güver, T.; Tomsick, J. A.; Buxton, M. M.; Brocksopp, C.; Corbel, S.; Cabrera-Lavers, A.

    2013-06-01

    Galactic black hole transients show many interesting phenomena during outburst decays. We present simultaneous X-ray (RXTE, Swift, and INTEGRAL), and optical/near-infrared (O/NIR) observations (SMARTS) of the X-ray transient XTE J1752-223 during its outburst decay in 2010. The multiwavelength observations over 150 days in 2010 cover the transition from soft to hard spectral state. We discuss the evolution of radio emission with respect to the O/NIR light curve which shows several flares. One of those flares is bright and long, starting about 60 days after the transition in X-ray timing properties. During this flare, the radio spectral index becomes harder. Other smaller flares occur along with the X-ray timing transition, and also right after the detection of the radio core. We discuss the significances of these flares. Furthermore, using the simultaneous broadband X-ray spectra including INTEGRAL, we find that a high energy cut-off with a folding energy near 250 keV is necessary around the time that the compact jet is forming. The broadband spectrum can be fitted equally well with a Comptonization model. In addition, using photoelectric absorption edges in the XMM-Newton Reflection Grating Spectrometer X-ray spectra and the extinction of red clump giants in the direction of the source, we find a lower limit on the distance of >5 kpc.

  4. An accurate position for the black hole candidate XTE J1752-223: re-interpretation of the VLBI data

    CERN Document Server

    Miller-Jones, J C A; Ratti, E M; Torres, M A P; Brocksopp, C; Yang, J; Morrell, N I

    2011-01-01

    Using high-precision astrometric optical observations from the Walter Baade Magellan Telescope in conjunction with high-resolution very long baseline interferometric (VLBI) radio imaging with the Very Long Baseline Array (VLBA), we have located the core of the X-ray binary system XTE J1752-223. Compact radio emission from the core was detected following the state transition from the soft to the hard X-ray state. Its position to the south-east of all previously-detected jet components mandated a re-analysis of the existing VLBI data. Our analysis suggests that the outburst comprised at least two ejection events prior to 2010 February 26. No radio-emitting components were detected to the south-east of the core at any epoch, suggesting that the receding jets were Doppler-deboosted below our sensitivity limit. From the ratio of the brightness of the detected components to the measured upper limits for the receding ejecta, we constrain the jet speed to be greater than 0.66c and the inclination angle to the line of...

  5. A 6-year Evaluation of 223 Tapered Dental Implants and associated prosthesis in 92 patients at a University Hospital.

    Science.gov (United States)

    Nagi, Sana Ehsen; Khan, Farhan Raza; Ali, Rabia

    2016-10-01

    The aim of the retrospective study was to assess the clinical and radiographic outcome of the dental implant surgery and prosthetics. It was conducted at the Aga Khan University Hospital, Karachi, and comprised of medical charts and radiographic records of patients visiting between 2010 and 2015. Variables such as implant dimensions, final prosthesis, method of retention, loading protocol and patient factors were analysed. A total of 223 implants (143(64.1%) in maxilla and 80(35.9%) in mandible) were placed in 92 subjects (50(54.3%) males and 42(45.7%) females). All implants were Zimmer tapered screw-vent. Length of 108(48.4%) implants was 11.5mm and diameter of 84(37.7%) implants was 4.7mm. Besides, 6(2.7%) implants failed to osseointegrate, whereas 1(0.4%) implant failed at 12 months of loading. Among the 216(96.9%) successful implants, 140(64.8%) served as bridge abutments, 72(33.3%) were single crown abutments and 4(1.9%) were overdenture abutments. Also, 37(17.1%) implants were immediately loaded. The six-year survival rate of implants was 96.9%.

  6. An isotope-edited FTIR investigation of the role of Ser-L223 in binding quinone (QB) and semiquinone (QB-) in the reaction center from Rhodobacter sphaeroides.

    Science.gov (United States)

    Nabedryk, Eliane; Paddock, Mark L; Okamura, Melvin Y; Breton, Jacques

    2005-11-08

    In the photosynthetic reaction center (RC) from the purple bacterium Rhodobacter sphaeroides, proton-coupled electron-transfer reactions occur at the secondary quinone (Q(B)) site. Several nearby residues are important for both binding and redox chemistry involved in the light-induced conversion from Q(B) to quinol Q(B)H(2). Ser-L223 is one of the functionally important residues located near Q(B). To obtain information on the interaction between Ser-L223 and Q(B) and Q(B)(-), isotope-edited Q(B)(-)/Q(B) FTIR difference spectra were measured in a mutant RC in which Ser-L223 is replaced with Ala and compared to the native RC. The isotope-edited IR fingerprint spectra for the C=O [see text] and C=C [see text] modes of Q(B) (Q(B)(-)) in the mutant are essentially the same as those of the native RC. These findings indicate that highly equivalent interactions of Q(B) and Q(B)(-) with the protein occur in both native and mutant RCs. The simplest explanation of these results is that Ser-L223 is not hydrogen bonded to Q(B) or Q(B)(-) but presumably forms a hydrogen bond to a nearby acid group, preferentially Asp-L213. The rotation of the Ser OH proton from Asp-L213 to Q(B)(-) is expected to be an important step in the proton transfer to the reduced quinone. In addition, the reduced quinone remains firmly bound, indicating that other distinct hydrogen bonds are more important for stabilizing Q(B)(-). Implications on the design features of the Q(B) binding site are discussed.

  7. 非小细胞肺癌血浆miR-223、miR-93和miR-218的表达及其临床意义%Expression and clinical value analysis of plasma miR-223, miR-93 and miR-218 in non-small lung cancer

    Institute of Scientific and Technical Information of China (English)

    蔡静清; 王宁; 张敏

    2014-01-01

    Objective To explore the levels of plasma miR⁃223, miR⁃93 and miR⁃218 in non⁃small lung cancer( NSCLC) and analysis the relationship between the 3 indicators and clinicopathological parameters of NSCLC. Methods Plasma samples from 85 NSCLC patients before treatment were collected as NSCLC group. The real⁃time quantitative RT⁃PCR( qRT⁃PCR) was used to detect the levels of miR⁃223, miR⁃93 and miR⁃218 and the clinicopathological parameters of NSCLC patients were collected. The clinicopatho⁃logical parameters of patients with different levels of miR⁃223, miR⁃93 and miR⁃218 were compared. The receiver operating characteris⁃tic curve( ROC) was employed to analysis the clinical value of plasma miR⁃223, miR⁃93 and miR⁃218 in the diagnosis of NSCLC. Meanwhile, the plasma samples from 90 healthy volunteers at the same time were selected as control( control group) . Results There were higher levels of miR⁃223 and miR⁃93 but lower miR⁃218 in NSCLC group compaired with control group with significant difference ( P<0�05) . The level of miR⁃223 was related with TNM stage and tumor size, and miR⁃93 was related with histological type and lymph node metastasis, and miR⁃218 was related with tumor size and degree of differentiation, showing with significant difference ( P all<0�05) . The plasma level of miR⁃223 was positively correlated with miR⁃93( r=0�411) , but miR⁃223 and miR⁃93 were negatively cor⁃related with miR⁃218(r=-0�361 and r=-0�451)with statistically significance(P<0�05). The AUC, sensitivity and specificity of plasma miR⁃223, miR⁃93 and miR⁃218 in the diagnosis of NSCLC were 0�926, 95�2% and 87�1%, 0�912, 88�4% and 92�5%, and 0�941, 92�7% and 84�4%, higher than 0�774, 75�1% and 66�2% of carcinoembryonic antigen( CEA) . The combined efficiency of miR⁃223, miR⁃93 and miR⁃218 in NSCLC was superior to that alone. Conclusion There are higher expression of miR⁃223 and

  8. Severe intellectual disability, omphalocele, hypospadia and high blood pressure associated to a deletion at 2q22.1q22.3: case report

    Directory of Open Access Journals (Sweden)

    Mulatinho Milene

    2012-06-01

    Full Text Available Abstract Background Recently, array-comparative genomic hybridization (aCGH platforms have significantly improved the resolution of chromosomal analysis allowing the identification of genomic copy number gains and losses smaller than 5 Mb. Here we report on a young man with unexplained severe mental retardation, autism spectrum disorder, congenital malformations comprising hypospadia and omphalocele, and episodes of high blood pressure. An ~ 6 Mb interstitial deletion that includes the causative genes is identified by oligonucleotide-based aCGH. Results Our index case exhibited a de novo chromosomal abnormality at 2q22 [del(2(q22.1q22.3dn] which was not visible at the 550 haploid band level. The deleted region includes eight genes: HNMT, SPOPL, NXPH2, LOC64702, LRP1B, KYNU, ARHGAP15 and GTDC1. Discussion aCGH revealed an ~ 6 Mb deletion in 2q22.1 to 2q22.3 in an as-yet unique clinical case associated with intellectual disability, congenital malformations and autism spectrum disorder. Interestingly, the deletion is co-localized with a fragile site (FRA2K, which could be involved in the formation of this chromosomal aberration. Further studies are needed to determine if deletions of 2q22.1 to 2q22.3 define a new microdeletion syndrome.

  9. The black hole candidate XTE J1752-223 towards and in quiescence: optical and simultaneous X-ray - radio observations

    CERN Document Server

    Ratti, E M; Miller-Jones, J C A; Torres, M A P; Homan, J; Markoff, S; Tomsick, J A; Kaaret, P; Wijnands, R; Gallo, E; Ozel, F; Steeghs, D T H; Fender, R P

    2012-01-01

    We present optical, X-ray and radio observations of the black hole transient (BHT) XTE J1752-223 towards and in quiescence. Optical photometry shows that the quiescent magnitude of XTE J1752-223 is fainter than 24.4 magnitudes in the i'-band. A comparison with measurements of the source during its 2009-2010 outburst shows that the outburst amplitude is more than 8 magnitudes in the i'-band. Known X-ray properties of the source combined with the faintness of the quiescence optical counterpart and the large outburst optical amplitude point towards a short orbital period system (Porb<~6.8 h) with an M type (or later) mass donor, at a distance of 3.5<~d<~8 kpc. Simultaneous X-ray and radio data were collected with Chandra and the EVLA, allowing constraints to be placed on the quiescent X-ray and radio flux of XTE J1752-223. Furthermore, using data covering the final stage of the outburst decay, we investigated the low luminosity end of the X-ray - radio correlation for this source and compared it with ot...

  10. RNA interference of endochitinases in the sugarcane endophyte Trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.

    Directory of Open Access Journals (Sweden)

    Aline S Romão-Dumaresq

    Full Text Available The sugarcane root endophyte Trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. Its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including N-acetyl-ß-D-glucosaminidases, chitobiosidases and endochitinases. We used targeted gene deletion and RNA-dependent gene silencing strategies to disrupt N-acetyl-ß-D-glucosaminidase and endochitinase activities of the fungus, and to determine their roles in the biocontrol of soil-borne plant pathogens. The loss of N-acetyl-ß-D-glucosaminidase activities was dispensable for biocontrol of the plurivorous damping-off pathogens Rhizoctonia solani and Sclerotinia sclerotiorum, and of the sugarcane pathogen Ceratocystis paradoxa, the causal agent of pineapple disease. Similarly, suppression of endochitinase activities had no effect on R. solani and S. sclerotiorum disease control, but had a pronounced effect on the ability of T. virens 223 to control pineapple disease. Our work demonstrates a critical requirement for T. virens 223 endochitinase activity in the biocontrol of C. paradoxa sugarcane disease, but not for general antagonism of other soil pathogens. This may reflect its lifestyle as a sugarcane root endophyte.

  11. ASSOCIATION BETWEEN GLN223ARG POLYMORPHISM IN LEPTIN RECEPTOR GENE AND OVERWEIGHT-OBESITY IN A CERTAIN DISTRICT IN SHANGHAI HAN NATIONALITY%LEPR基因GIn223Arg多态性与上海某区汉族儿童的相关性研究

    Institute of Scientific and Technical Information of China (English)

    江山曦; 郭红卫; 薛琨; 朱美英; 朱俭; 罗飞宏

    2012-01-01

    目的 探究瘦素受体基因Gln223Arg多态性与上海某区儿童超重肥胖的关系及相关生化指标的相关性.方法 采用PCR-RFLP对上海松江区六所小学7-11岁超重肥胖与正常体重儿童(各262名)的血样进行LEPR基因Gln223Arg多态性检测,logistic回归分析其与儿童超重肥胖的相关性,并分析了生化指标在超重肥胖组与正常组儿童以及不同基因型间的差异. 结果 超重肥胖组与对照组之间LEPR基因Gln223Arg从频率与A等位基因频率均无统计学差异(P>0.05).对其收缩压(SBP),舒张压(DBP),胆固醇(TC),甘油三酯(TG),血糖(GLU),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)进行t检验,得出SBP,DBP,TG,HDL-C和LDL-C在超重肥胖组与对照组中差异显著.在控制了混杂因素BMI等后,LEPR基因Gln223Arg各基因型组的生化指标差异分析表明基因型可能与LDL-C相关(P<0.05).进一步两两比较结果表明LDL-C水平在基因型AA组比AG(P=0.019)与GG组(P=0.017)低,而AG与GG组之间的差异无统计学意义(P=0.517). 结论 LEPR基因Gln223Arg多态性可能与儿童超重肥胖的发生无关,而LDL-C(P<0.05)可能与LEPR基因Gln223Arg基因型有相关性.%Objective To evaluate the association between leptin receptor (LEPR) Gln223Arg polymorphism and overweight-obesity in children, as well as related biochemical indices. Method The subjects were from six primary schools with the age from seven to eleven, in Songjiang District, Shanghai, China. LEPR Gln223Arg polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism in 262 normal weight subjects and 262 overweight-obese subjects. And logistic regression was used for analysis of the LEPR Gln223Arg polymorphism with obesity. Difference of biochemical indices was analyzed between the two groups, as well as between different genotypes. Results There was no significant difference of AA genotype and A allele of LEPR gene between

  12. Isolation of Actinium from Neutron-irradiated Thorium-I

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    Isolation of Actinium from Neutron-irradiated Thorium-I¥YangWeifan;YuanShuanggui;MuWantong;ZhangXueqian;LiZhongweiandZhaoLili...

  13. Production of actinium-225 for alpha particle mediated radioimmunotherapy.

    Science.gov (United States)

    Boll, Rose A; Malkemus, Dairin; Mirzadeh, Saed

    2005-05-01

    The initial clinical trials for treatment of acute myeloid leukemia have demonstrated the effectiveness of the alpha emitter (213)Bi in killing cancer cells. Bismuth-213 is obtained from a radionuclide generator system from decay of 10-days (225)Ac parent. Recent pre-clinical studies have also shown the potential application of both (213)Bi, and the (225)Ac parent radionuclide in a variety of cancer systems and targeted radiotherapy. This paper describes our five years of experience in production of (225)Ac in partial support of the on-going clinical trials. A four-step chemical process, consisting of both anion and cation exchange chromatography, is utilized for routine separation of carrier-free (225)Ac from a mixture of (228)Th, (229)Th and (232)Th. The separation of Ra and Ac from Th is achieved using the marcoporous anion exchange resin MP1 in 8M HNO(3) media. Two sequential MP1/NO(3) columns provide a separation factor of approximately 10(6) for Ra and Ac from Th. The separation of Ac from Ra is accomplished on a low cross-linking cation exchange resin AG50-X4 using 1.2M HNO(3) as eluant. Two sequential AG50/NO(3) columns provide a separation factor of approximately 10(2) for Ac from Ra. A 60-day processing schedule has been adopted in order to reduce the processing cost and to provide the highest levels of (225)Ac possible. Over an 8-week campaign, a total of approximately 100 mCi of (225)Ac (approximately 80% of the theoretical yield) is shipped in 5-6 batches, with the first batch typically consisting of approximately 50 mCi. After the initial separation and purification of Ac, the Ra pool is re-processed on a bi-weekly schedule or as needed to provide smaller batches of (225)Ac. The averaged radioisotopic purity of the (225)Ac was 99.6 +/- 0.7% with a (225)Ra content of < or =0.6%, and an average (229)Th content of (4(-4)(+5)) x 10(-5)%.

  14. Development in the study of radium-223 chloride for treating castration-resistant prostate carcinoma with bone metastases%镭-223氯化物治疗去势抵抗性前列腺癌骨转移的研究进展

    Institute of Scientific and Technical Information of China (English)

    桂继琮; 刘兴党

    2015-01-01

    近年来,全世界前列腺癌的发病率和病死率不断增长,各种治疗方法的研究也进展迅速。镭-223发射的重α粒子(具有<100μm的超短波)靶向作用于骨转移部位和周围新骨生长区域。镭-223氯化物在药物临床试验中以稳定的安全性显著降低了患者的病死率,成为近来被寄予厚望的转移性去势抵抗性前列腺癌(CRPC)治疗领域的“主角”。2013年美国食品药品管理局已批准镭-223氯化物用于治疗有症状的骨转移、未发现内脏转移的CRPC患者。未来其联合治疗方案将会是研究的重点。%Prostate carcinoma rates and mortalities are increasing worldwide. The therapeutic landscape of castration-resistant prostate carcinoma (CRPC) has changed rapidly. Radium-223 chloride, which is a radiopharmaceutical agent, targets bone metastasis by emitting high-energy alpha-particles with extremely short range (<100 μm ) . This chemical is effective in reducing mortality without increasing toxicity. Thus, this agent has the potential to become a new alternative for treating patients with CRPC. The Food and Drug Administration approved radium-223 dichloride for treating patients with CRPC, symptomatic bone metastases, and unknown visceral metastatic disease in 2013. Therefore, combination and sequencing strategies will be future research directions.

  15. TCM Tongue-appearance Analysis and Pathology Discussion on 223 HIV/AIDS Patients in Different Periods of Disease%223例HIV/AIDS患者不同分期中医舌象分析与病机探讨

    Institute of Scientific and Technical Information of China (English)

    陈昕; 田春; 陈玉珍

    2008-01-01

    目的 从舌象变化分析归纳云南地区HIV/AIDS患者中医证候及病机特点.方法 对223例初次自愿接受中医药治疗或咨询的云南地区HIV/AIDS患者进行,临床分期后观察舌象.结果 通过对艾滋病期、无症状期患者舌质色分布,舌形态及舌苔质色的观察,各期的病理舌象均以腻苔为主;艾滋病期以白腻苔(47.45%)为主,其次为黄腻苔(16.06%)、薄黄苔(8.02%),而舌质色以淡舌类(淡红舌与淡白舌合计46.71%)居多,其次为红舌类(32.12%)、瘀舌类(21.17%);无症状期均以淡红舌、薄白苔为主,比例均大于艾滋病期,免疫水平相对较高时,正常舌象表现越多.无症状期A组(200≤CD4<500)红舌类(42.65%)较无症状期B组(CD4>500)红舌类(6%)居多.结论 云南地区HIV/AIDS患者证候和病机特点为:邪实以湿毒秽浊痰饮为主,正虚以脾气阳虚,肺气阴两虚、肾阴阳亏虚为主.

  16. Open clusters. II. Fundamental parameters of B stars in Collinder 223, Hogg 16, NGC 2645, NGC 3114, and NGC 6025

    Science.gov (United States)

    Aidelman, Y.; Cidale, L. S.; Zorec, J.; Panei, J. A.

    2015-05-01

    Context. The knowledge of accurate values of effective temperature, surface gravity, and luminosity of stars in open clusters is very important not only to derive cluster distances and ages but also to discuss the stellar structure and evolution. Unfortunately, stellar parameters are still very scarce. Aims: Our goal is to study five open clusters to derive stellar parameters of the B and Be star population and discuss the cluster properties. In a near future, we intend to gather a statistically relevant samples of Be stars to discuss their origin and evolution. Methods: We use the Barbier-Chalonge-Divan spectrophotometric system, based on the study of low-resolution spectra around the Balmer discontinuity, since it is independent of the interstellar and circumstellar extinction and provides accurate Hertzsprung-Russell diagrams and stellar parameters. Results: We determine stellar fundamental parameters, such as effective temperatures, surface gravities, spectral types, luminosity classes, absolute and bolometric magnitudes and colour gradient excesses of the stars in the field of Collinder 223, Hogg 16, NGC 2645, NGC 3114, and NGC 6025. Additional information, mainly masses and ages of cluster stellar populations, is obtained using stellar evolution models. In most cases, stellar fundamental parameters have been derived for the first time. We also discuss the derived cluster properties of reddening, age and distance. Conclusions: Collinder 223 cluster parameters are overline{E(B-V) = 0.25 ± 0.03} mag and overline{(mv - M_v)0 = 11.21 ± 0.25} mag. In Hogg 16, we clearly distinguish two groups of stars (Hogg 16a and Hogg 16b) with very different mean true distance moduli (8.91 ± 0.26 mag and 12.51 ± 0.38 mag), mean colour excesses (0.26 ± 0.03 mag and 0.63 ± 0.08 mag), and spectral types (B early-type and B late-/A-type stars, respectively). The farthest group could be merged with Collinder 272. NGC 2645 is a young cluster (<14 Myr) with overline{E(B-V) = 0

  17. Do clinicians and patients agree regarding symptoms? A comparison after definitive radiochemotherapy in 223 uterine cervical cancer patients

    Energy Technology Data Exchange (ETDEWEB)

    Kirchheiner, K.; Poetter, R. [Medical Univ. Vienna (Austria). Dept. of Radiotherapy; Nout, R. [University Medical Center Leiden (Netherlands). Dept. of Clinical Oncology; Lindegaard, J. [University Hospital Aarhus (Denmark). Dept. of Oncology; Petric, P. [Institute of Oncology Ljubljana (Slovenia). Dept. of Radiotherapy; Limbergen, E.V. [University Hospital Leuven (Belgium). Dept. of Radiotherapy; Juergenliemk-Schulz, I.M. [University Medical Center Utrecht (Netherlands). Dept. of Radiation Oncology; Haie-Meder, C. [Institut Gustave-Roussy, Villejuif (France). Dept. of Radiotherapy; Doerr, W. [Technische Univ. Dresden (Germany). Dept. of Radiotherapy and Radiooncology

    2012-10-15

    Background: In clinical cancer research of morbidity, low associations between clinician-assessed toxicity/morbidity and patient-reported symptoms are consistently described in the literature. While morbidity grading systems are supposed to follow more or less objective criteria, patient reported symptoms inherently are based on a subjective self-evaluation of the impact on quality of life. The aim of this study was to focus on major discrepancies with high clinical relevance and to evaluate its impact with regard to underreporting of morbidity. Material and methods: Early morbidity assessed by clinicians with CTCAEv.3 and patient reported quality of life (EORTC-QLQ-C30/CX24) were compared regarding 12 overlapping symptoms in 223 patients with uterine cervical cancer 3 months after definitive radio(chemo)therapy in the ongoing EMBRACE study. Mismatches showing discrepancies between both grading systems were classified, if patients reported substantial symptoms (quite a bit/very much) and CTCAE grading was rated G0. Results: In total, 360 substantial symptoms were reported by patients by EORTC-QLQ; 159 (44%) of those were not recognized by CTCAE. Symptoms with the highest occurrence of mismatches overall are urinary frequency, fatigue, and insomnia. Large institutional differences were found, showing two centers with 4 vs. 71% of patients with at least one mismatch. Conclusion: Analysis of mismatches indicated a high risk of underestimation of early morbidity. Thus, nearly half of the patient-reported substantial symptoms were not recognized by CTCAE scoring (G0) 3 months after treatment. Prospective assessment of morbidity in clinical studies should, therefore, integrate patient reported symptoms to receive a complete and comprehensive picture. (orig.)

  18. The nearby spiral density-wave structure of the Galaxy: line-of-sight and longitudinal velocities of 223 Cepheids

    Science.gov (United States)

    Griv, Evgeny; Hou, Li-Gang; Jiang, Ing-Guey; Ngeow, Chow-Choong

    2017-02-01

    The data of 223 stars within 4 kpc from the Sun and 400 pc from the plane are collected from Melnik et al., who listed photometric distances and velocities of 674 long-period Cepheids in the disc of our Galaxy. Both line-of-sight and transverse along the Galactic longitude velocities of the selected stars are analysed on the assumption that the system is the subject to Lin-Shu-type moderately unstable, low-amplitude, tightly wound and rigidly rotating density waves. Minimization of the least-squares estimator S of measured and modelled velocities of stars was performed in the geometrical parameters of waves. The wave structures defined from the two independent samples of velocities are nearly similar. Different radial scales (wavelengths) of velocity inhomogeneity of about 1, 2 and 4 kpc, which do not depend on the number of spiral arms m, were found. But for now it is unclear if one can identify these scales as fully independent global modes of collective oscillations or they are Fourier harmonics of a single wave, or they just reveal the segments of large-scale Orion, Sagittarius and Perseus arms. The dominant mode is the one-armed wave for the main minimum of all S considered. However, because the S values for fits with m = 1-4 are almost equal the latter conclusion must be regarded as tentative until more extensive data are available. High-precision Gaia observations could reveal in the near future whether the wave-like velocity fluctuations near the Sun reported here do indeed exist.

  19. The CALYMHA survey: Lyα escape fraction and its dependence on galaxy properties at z = 2.23

    Science.gov (United States)

    Matthee, Jorryt; Sobral, David; Oteo, Iván; Best, Philip; Smail, Ian; Röttgering, Huub; Paulino-Afonso, Ana

    2016-05-01

    We present the first results from our CAlibrating LYMan α with Hα (CALYMHA) pilot survey at the Isaac Newton Telescope. We measure Lyα emission for 488 Hα selected galaxies at z = 2.23 from High-z Emission Line Survey in the COSMOS and UDS fields with a specially designed narrow-band filter (λc = 3918 Å, Δλ = 52 Å). We find 17 dual Hα-Lyα emitters [fLyα > 5 × 10-17 erg s-1 cm-2, of which five are X-ray active galactic nuclei (AGN)]. For star-forming galaxies, we find a range of Lyα escape fractions (fesc, measured with 3 arcsec apertures) from 2 to 30 per cent. These galaxies have masses from 3 × 108 M⊙ to 1011 M⊙ and dust attenuations E(B - V) = 0-0.5. Using stacking, we measure a median escape fraction of 1.6 ± 0.5 per cent (4.0 ± 1.0 per cent without correcting Hα for dust), but show that this depends on galaxy properties. The stacked fesc tends to decrease with increasing star formation rate and dust attenuation. However, at the highest masses and dust attenuations, we detect individual galaxies with fesc much higher than the typical values from stacking, indicating significant scatter in the values of fesc. Relations between fesc and UV slope are bimodal, with high fesc for either the bluest or reddest galaxies. We speculate that this bimodality and large scatter in the values of fesc is due to additional physical mechanisms such as outflows facilitating fesc for dusty/massive systems. Lyα is significantly more extended than Hα and the UV. fesc continues to increase up to at least 20 kpc (3σ, 40 kpc [2σ]) for typical star-forming galaxies and thus the aperture is the most important predictor of fesc.

  20. An accurate position for the black hole candidate XTE J1752-223: re-interpretation of the VLBI data

    Science.gov (United States)

    Miller-Jones, J. C. A.; Jonker, P. G.; Ratti, E. M.; Torres, M. A. P.; Brocksopp, C.; Yang, J.; Morrell, N. I.

    2011-07-01

    Using high-precision astrometric optical observations from the Walter Baade Magellan Telescope in conjunction with high-resolution very long baseline interferometric (VLBI) radio imaging with the Very Long Baseline Array (VLBA), we have located the core of the X-ray binary system XTE J1752-223. Compact radio emission from the core was detected following the state transition from the soft to the hard X-ray state. Its position to the south-east of all previously detected jet components mandated a re-analysis of the existing VLBI data. Our analysis suggests that the outburst comprised at least two ejection events prior to 2010 February 26. No radio-emitting components were detected to the south-east of the core at any epoch, suggesting that the receding jets were Doppler-deboosted below our sensitivity limit. From the ratio of the brightness of the detected components to the measured upper limits for the receding ejecta, we constrain the jet speed β > 0.66 and the inclination angle to the line of sight θ < 49°. Assuming that the initial ejection event occurred at the transition from the hard intermediate state to the soft intermediate state, an initial period of ballistic motion followed by a Sedov phase (i.e. self-similar adiabatic expansion) appears to fit the motion of the ejecta better than a uniform deceleration model. The accurate core location can provide a long time baseline for a future proper motion determination should the system show a second outburst, providing insights into the formation mechanism of the compact object.

  1. MO-AB-201-01: Regulatory Compliance and Safety with New Radiotherapies: Spheres and Ra-223

    Energy Technology Data Exchange (ETDEWEB)

    Phillips, L. [Stanford University (United States)

    2015-06-15

    The role of the Radiation Safety Officer at a medical facility can be complicated. The complexity of the position is based on the breadth of services provided at the institution and the nature of the radioactive materials license. Medical practices are constantly changing and the use of ionizing radiation continues to rise in this area. Some of the newer medical applications involving radiation have unique regulatory and safety issues that must be addressed. Oversight of the uses of radiation start at the local level (radiation safety officer, radiation safety committee) and are heavily impacted by outside agencies (i.e. Nuclear Regulatory Commission, State Radiologic Health, The Joint Commission (TJC), etc). This session will provide both an overview of regulatory oversight and essential compliance practices as well as practical ways to assess and introduce some of the new applications utilizing radioactive materials into your medical facility. Learning Objectives: Regulatory Compliance and Safety with New Radiotherapies: Spheres and Ra-223 (Lance Phillips) Understand the radioactive materials license amendment process to add new radiotherapies (i.e., SIR-Spheres, Therasphere, Xofigo). Understand the AU approval process for microspheres and Xofigo. Examine the training and handling requirements for new procedures. Understand the process involved with protocol development, SOP in order to define roles and responsibilities. The RSO and The RSC: Challenges and Opportunities (Colin Dimock) Understand how to form an effective Committee. Examine what the Committee does for the Program and the RSO. Understand the importance of Committee engagement. Discuss the balance of the complimentary roles of the RSO and the Committee. The Alphabet Soup of Regulatory Compliance: Being Prepared for Inspections (Linda Kroger) Recognize the various regulatory bodies and organizations with oversight or impact in Nuclear Medicine, Radiology and Radiation Oncology. Examine 10CFR35

  2. Factors associated with multimorbidity and its link with poor blood pressure control among 223,286 hypertensive patients.

    Science.gov (United States)

    Wong, Martin C S; Wang, Harry H X; Cheung, Clement S K; Tong, Ellen L H; Sek, Antonio C H; Cheung, N T; Yan, Bryan P Y; Yu, Cheuk-Man; Griffiths, Sian M; Coats, Andrew J S

    2014-11-15

    Multimorbidity has become the norm worldwide as populations age. It remains, however, infrequently researched. This study evaluated factors associated with multimorbidity in a predominantly Chinese hypertensive population. We included all adult patients prescribed their first antihypertensive agents in the entire public sector in Hong Kong from a validated database. Multimorbidity was defined as having one or more medical conditions (cardiovascular diseases; respiratory diseases; diabetes or impaired fasting glucose; renal disease) in addition to hypertension. We studied the prevalence of multimorbidity and performed multinomial regression analyses to evaluate factors independently associated with multimorbidity. 223,286 hypertensive patients (average age of 59.9 years, SD 17.6) were included. The prevalence of having 0, 1 and ≥ 2 additional conditions was 59.6%, 32.8% and 7.5%, respectively. The most common conditions were cardiovascular disease (24.2%) and diabetes (23.0%), followed by respiratory disorders (14.6%) and renal disease (10.9%). Older age (>50 years), male sex, lower household income, receipt of social security allowance and suboptimal blood pressure control (>140 mmHg or >90 mmHg; >130 mmHg or >80 mmHg for diabetes patients; AOR = 3.38-4.49) were significantly associated with multimorbidity. There exists a synergistic effect among these variables as older (≥ 70 years), male patients receiving security allowance had substantially higher prevalence of multimorbidity (19.9% vs 7.5% among all patients). Multimorbidity is very common in hypertensive patients and its prevalence increased markedly with the presence of risk factors identified in this study. Hypertensive patients with multimorbidities should receive more meticulous clinical care as their blood pressure control tends to be poorer.

  3. MicroRNA-223在淋巴细胞白血病原代细胞中表达及作用机制的研究%Expression of MicroRNA-223 in Lymphocytic Leukemia Cells and Its Action Mechanism

    Institute of Scientific and Technical Information of China (English)

    南祯; 梁勇; 付蓉; 刘惠; 阮二宝; 王晓明; 王国锦; 瞿文; 刘鸿

    2013-01-01

    本研究旨在探讨急性淋巴细胞白血病(ALL)、慢性淋巴细胞白血病(CLL)原代细胞中MicroRNA-223与LMO2基因的表达水平及MicroRNA-223的作用机制.应用人淋巴细胞分离液分选ALL、CLL患者及正常人骨髓中淋巴细胞,在ALL、CLL患者骨髓淋巴细胞中转染MicroRNA-223的类似物靶向升高细胞中MicroRNA-223的表达,在正常人骨髓淋巴细胞中转染MicroRNA-223抑制物靶向敲低细胞中MicroRNA-223的表达.转染后培养72 h,用RT-PCR方法检测转染前后MicroRNA-223和LMO2表达量及其相关性,并用流式细胞术进一步观察细胞凋亡和细胞周期的变化.结果表明,转染MicroRNA-223类似物前,ALL、CLL患者中MicroRNA-223表达水平为(433.11±144.88),LMO2水平为(807.10±238.41),正常人转染MicroRNA-223抑制物前,MicroRNA-223表达水平为(949.59±267.39),LMO2的表达为(455.32±176.83);MicrRNA-223的表达在正常人中明显高于ALL、CLL患者(P<0.05),而LMO2的表达在正常人中明显低于ALL、CLL患者(P<0.05).转染后,ALL、CLL患者中MicroRNA-223表达明显增加(571.86±142.00) (P <0.05),而LMO2表达明显减少(651.97±230.12) (P <0.05);在正常人中MicroRNA-223表达明显降低(646.32±172.93) (P <0.05),LMO2的表达明显升高(541.27±158.86) (P <0.05).转染后细胞周期及细胞凋亡率的变化表现为,在ALL、CLL患者转染前细胞周期G1/G2细胞比例为(94.75±3.15)%,S期为(5.14±3.12)%;转染后G1/G2细胞比例明显增加(97.03±2.08)% (P <0.05),在S期明显减少(2.97±2.08)%(P<0.05);转染前细胞凋亡率为(54.47±8.72)%,转染后为(60.48±8.81)%,后者明显增加(P<0.05).正常人转染前细胞周期G1/G2细胞比例是(96.73±2.26)%,S期是(3.25±2.26)%;转染后G1/G2细胞比例明显减少(94.55±2.77)%(P<0.05),在S期明显增加(5.45±2.77)%(P<0.05),细胞凋亡率为(59.02±10.20)%,转染后明显减少(51.96±10.20)% (P <0.05).结论:淋巴细胞

  4. I223R mutation in influenza A(H1N1pdm09 neuraminidase confers reduced susceptibility to oseltamivir and zanamivir and enhanced resistance with H275Y.

    Directory of Open Access Journals (Sweden)

    Jérome LeGoff

    Full Text Available BACKGROUND: Resistance of pandemic A(H1N12009 (H1N1pdm09 virus to neuraminidase inhibitors (NAIs has remained limited. A new mutation I223R in the neuraminidase (NA of H1N1pdm09 virus has been reported along with H275Y in immunocompromised patients. The aim of this study was to determine the impact of I223R on oseltamivir and zanamivir susceptibility. METHODS: The NA enzymatic characteristics and susceptibility to NAIs of viruses harbouring the mutations I223R and H275Y alone or in combination were analyzed on viruses produced by reverse genetics and on clinical isolates collected from an immunocompromised patient with sustained influenza H1N1pdm09 virus shedding and treated by oseltamivir (days 0-15 and zanamivir (days 15-25 and 70-80. RESULTS: Compared with the wild type, the NA of recombinant viruses and clinical isolates with H275Y or I223R mutations had about two-fold reduced affinity for the substrate. The H275Y and I223R isolates showed decreased susceptibility to oseltamivir (246-fold and oseltamivir and zanamivir (8.9- and 4.9-fold, respectively. Reverse genetics assays confirmed these results and further showed that the double mutation H275Y and I223R conferred enhanced levels of resistance to oseltamivir and zanamivir (6195- and 15.2-fold. In the patient, six days after initiation of oseltamivir therapy, the mutation H275Y conferring oseltamivir resistance and the I223R mutation were detected in the NA. Mutations were detected concomitantly from day 6-69 but molecular cloning did not show any variant harbouring both mutations. Despite cessation of NAI treatment, the mutation I223R persisted along with additional mutations in the NA and the hemagglutinin. CONCLUSIONS: Reduced susceptibility to both oseltamivir and zanamivir was conferred by the I223R mutation which potentiated resistance to both NAIs when associated with the H275Y mutation in the NA. Concomitant emergence of the I223R and H275Y mutations under oseltamivir treatment

  5. Ischemic brain extract increases SDF-1 expression in astrocytes through the CXCR2/miR-223/miR-27b pathway.

    Science.gov (United States)

    Shin, Jin Hee; Park, Young Mi; Kim, Dong Hee; Moon, Gyeong Joon; Bang, Oh Young; Ohn, Takbum; Kim, Hyeon Ho

    2014-09-01

    Ischemic cerebral stroke is one of the leading global causes of mortality and morbidity. Ischemic preconditioning (IPC) refers to a sublethal ischemia and resulting in tolerance to subsequent severe ischemic injury. Although several pathways are reportedly involved in IPC-mediated neuroprotection, the functional role of astrocytes is not fully understood. Stromal cell-derived factor-1 (SDF-1), a CXC chemokine produced mainly in astrocytes, is a ligand for chemokine receptor CXCR4. SDF-1 is reported to play a critical role in neuroprotection after stroke by mediating the migration of neuronal progenitor cells. We hypothesized that stimuli derived from ischemic brain were involved in the protective effects of IPC. To investigate this hypothesis, the mechanism in which ischemic brain extract (IBE) induced SDF-1 expression was investigated in C6 astrocytoma cells. IBE treatment of C6 cells increased SDF-1 expression compared to that in untreated or normal brain extract (NBE)-treated cells by downregulating SDF-1 targeting miRNA, miR-27b. MiR-223 was inversely upregulated in IBE-treated cells; overexpression of miR-223 decreased the expression of miR-27b by suppressing IKKα expression. Analysis of cytokine array data revealed an IBE associated enhanced expression of CINC-1 (CXCL1) and LIX1 (CXCL5). Knockdown or inhibition of their receptor, CXCR2, abolished IBE-mediated increased expression of SDF-1. These results were confirmed in primary cultured astrocytes. Taken together, the data demonstrate that IBE-elicited signals increase SDF-1 expression through the CXCR2/miR-223/miR-27b pathway in C6 astrocytoma cells and primary astrocytes, supporting the view that increased expression of SDF-1 by ischemic insults is a possible mechanism underlying therapeutic application of IPC.

  6. Post-traumatic stress symptoms and post-traumatic growth in 223 childhood cancer survivors: predictive risk factors

    Directory of Open Access Journals (Sweden)

    Marta eTremolada

    2016-02-01

    Full Text Available With modern therapies and supportive care, survival rates of childhood cancer have increased considerably. However, there are long-term psychological sequelae of these treatments that may not manifest until pediatric survivors are into adulthood. The prevalence of post-traumatic stress disorder (PTSD in young adult survivors of childhood cancer ranges from 6.2% to 22%; associated risk factors are young age at the assessment, female gender, low education level and some disease-related factors. The aim of this study was to investigate, in adolescent and young adult (AYA survivors of childhood cancer, the incidence and severity of post-traumatic stress symptoms (PTSS, and to identify the risk factors and the associated post-traumatic growth (PTG index.Participants were 223 AYA cancer survivors recruited during follow-up visits in the Oncohematology Clinic of the Department of Child and Woman’s Health, University of Padua. Data were collected from self-report questionnaires on PTSS incidence, PTG mean score, perceived social support, and medical and socio-demographic factors. Ex-patients’ mean age at the assessment was 19.33 years (SD = 3.01, 15-25, 123 males and 100 females, with a mean of years off-therapy of 9.64 (SD=4.17. Most (52.5% had survived an hematological disorder and 47.5% a solid tumor when they were aged, on average, 8.02 years (SD=4.40.The main results indicated a moderate presence of clinical (≥9 symptoms: 9.4% and sub-clinical PTSS (6-8 symptoms: 11.2%, with the avoidance criterion most often encountered. Re-experience symptoms and PTG mean score were significantly associated (r=0.24 p=0.0001. A hierarchical regression model (R2 = 0.08; F = 1.46; p = 0.05 identified female gender (β = 0.16; p = 0.05 and less perceived social support (β = -0.43; p = 0.05 as risk factors to developing PTSS. Another hierarchical regression model assessed the possible predictors of the PTG total score (R2 = 0.36; F = 9.1; p = 0.0001, with

  7. Post-traumatic Stress Symptoms and Post-traumatic Growth in 223 Childhood Cancer Survivors: Predictive Risk Factors.

    Science.gov (United States)

    Tremolada, Marta; Bonichini, Sabrina; Basso, Giuseppe; Pillon, Marta

    2016-01-01

    With modern therapies and supportive care, survival rates of childhood cancer have increased considerably. However, there are long-term psychological sequelae of these treatments that may not manifest until pediatric survivors are into adulthood. The prevalence of post-traumatic stress disorder in young adult survivors of childhood cancer ranges from 6.2 to 22%; associated risk factors are young age at the assessment, female gender, low education level, and some disease-related factors. The aim of this study was to investigate, in adolescent and young adult (AYA) survivors of childhood cancer, the incidence and severity of post-traumatic stress symptoms (PTSSs), and to identify the risk factors and the associated post-traumatic growth (PTG) index. Participants were 223 AYA cancer survivors recruited during follow-up visits in the Oncohematology Clinic of the Department of Child and Woman's Health, University of Padua. Data were collected from self-report questionnaires on PTSS incidence, PTG mean score, perceived social support, and medical and socio-demographic factors. Ex-patients' mean age at the assessment was 19.33 years (SD = 3.01, 15-25), 123 males and 100 females, with a mean of years off-therapy of 9.64 (SD = 4.17). Most (52.5%) had survived an hematological disorder and 47.5% a solid tumor when they were aged, on average, 8.02 years (SD = 4.40). The main results indicated a moderate presence of clinical (≥9 symptoms: 9.4%) and sub-clinical PTSS (6-8 symptoms: 11.2%), with the avoidance criterion most often encountered. Re-experience symptoms and PTG mean score were significantly associated (r = 0.24; p = 0.0001). A hierarchical regression model (R (2) = 0.08; F = 1.46; p = 0.05) identified female gender (β = 0.16; p = 0.05) and less perceived social support (β = -0.43; p = 0.05) as risk factors to developing PTSS. Another hierarchical regression model assessed the possible predictors of the PTG total score (R (2) = 0.36; F = 9.1; p = 0.0001), with

  8. Reflections on the therapeutic use of {sup 223}RaCl{sub 2} for bone metastases resulting from prostate cancer resistant to castration; Reflexiones sobre el uso terapeutico de {sup 223}RaCl{sub 2} para metastasis osea derivada de cancer de prostata resistente a la castracion

    Energy Technology Data Exchange (ETDEWEB)

    Astudillo V, A. J.; Paredes G, L., E-mail: armando.astudillo@inin.gob.mx [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)

    2015-10-15

    In January 2014 the Comision Federal para la Proteccion contra Riesgos Sanitarios of the Ministry of Health in Mexico, authorize the use of {sup 223}RaCl{sub 2} as the first radiopharmaceutical emitter α for therapeutic purposes in cases of bone metastases resulting from prostate cancer resistant to castration. The paper analyzes the main variables that affect the metrological traceability using activity meters to evaluate the gamma activity of {sup 223}RaCl{sub 2} in hospitals, because it has a chain of complex decay with alpha, beta and gamma emitters, so was important to verify if a gamma activity measurement for a multiple emitter is reliable to determine the total alpha absorbed dose to bone in a patient. (Author)

  9. Newly developed semi-empirical formulas for (, ) at 17.9 MeV and (, ) at 22.3 MeV reaction cross-sections

    Indian Academy of Sciences (India)

    Eyyup Tel; Abdullah Aydin; E Gamze Aydin; Abdullah Kaplan; Ömer Yavaş; İskender A Reyhancan

    2010-06-01

    In this study, we have investigated the asymmetry term effect for the (, ) and (, ) reaction cross-sections and obtained new coefficients for the (, ) and (, ) reaction cross-sections at 17.9 and 22.3 MeV energies. We have suggested semi-empirical formulas including the non-elastic effects of optical model found by fitting two parameters for proton-induced reactions. The coefficients were determined by least-square fitting method. The obtained cross-section formulas with new coefficients have been discussed and compared with the available experimental data.

  10. Oxidized Low-density Lipoprotein (ox-LDL) Cholesterol Induces the Expression of miRNA-223 and L-type Calcium Channel Protein in Atrial Fibrillation

    OpenAIRE

    Fengping He; Xin Xu; Shuguo Yuan; Liangqiu Tan; Lingjun Gao; Shaochun Ma; Shebin Zhang; Zhanzhong Ma; Wei Jiang; Fenglian Liu; Baofeng Chen; Beibei Zhang; Jungang Pang; Xiuyan Huang; Jiaqiang Weng

    2016-01-01

    Atrial fibrillation (AF) is the most common sustained arrhythmia causing high morbidity and mortality. While changing of the cellular calcium homeostasis plays a critical role in AF, the L-type calcium channel α1c protein has suggested as an important regulator of reentrant spiral dynamics and is a major component of AF-related electrical remodeling. Our computational modeling predicted that miRNA-223 may regulate the CACNA1C gene which encodes the cardiac L-type calcium channel α1c subunit. ...

  11. catena-Poly[[bis-[2-(2,3-dimethyl-anilino)benzoato-κO]cadmium(II)]-di-μ-3-pyridylmethanol-κN:O;κO:N].

    Science.gov (United States)

    Moncol, Jan; Mikloš, Dušan; Segľa, Peter; Koman, Marian; Lis, Tadeusz

    2008-02-06

    In the crystal structure of the title compound, [Cd(C(15)H(14)NO(2))(2)(C(6)H(7)NO)(2)](n), the Cd atom displays a distorted octa-hedral geometry, including two pyridine N atoms and two hydroxyl O from four symmetry-related 3-pyridylmethanol (3-pyme) ligands and two carboxylate O atoms from mefenamate [2-(2,3-dimethyl-anilino)benzoate] anions. The Cd atoms are connected via the bridging 3-pyme ligands into chains, that extend in the a-axis direction. The Cd atom is located on a center of inversion, whereas the 3-pyme ligands and the mefenamate anions occupy general positions.

  12. Leptin Receptor Gene Gln223Arg Polymorphism Is Not Associated with Hypertension: A Preliminary Population-Based Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Geórgia das Graças Pena

    2014-01-01

    Full Text Available Hypertension is responsible for high morbidity and mortality as one of the most important cardiometabolic risk factors. The aim of the study was to investigate whether the Gln223Arg in the leptin receptor (LEPR influences the prevalence of hypertension. A cross-sectional study was carried out in individuals aged ≥ 18 years. Polymorphism identification was performed using PCR-RFLP analysis. Participants with blood pressure ≥ 140/90 mmHg or medication use were considered hypertensive. Frequencies, means, cross-tabulations, and multivariate models were produced to study differences in hypertension prevalence by genotypes. The study includes 470 participants. The frequency of GG polymorphism variant was 10.43%, 46.81% AG, and 42.77% AA. The distribution of hypertension frequency by LEPR genotypes was the following: AA 43.8%, AG 40.4%, and GG 40.8%; there were no significant differences between groups. Comparative analysis which used multivariate Poisson regression adjusted by many potential confounders (age, sex, schooling, smoking, alcohol intake, obesity, and family history of parental obesity did not modify this result. In this large sample of population-based study, the association of the LEPR Gln223Arg gene polymorphism with hypertension was not observed.

  13. An Xq22.3 duplication detected by comparative genomic hybridization microarray (Array-CGH) defines a new locus (FGS5) for FG syndrome.

    Science.gov (United States)

    Jehee, Fernanda Sarquis; Rosenberg, Carla; Krepischi-Santos, Ana Cristina; Kok, Fernando; Knijnenburg, Jeroen; Froyen, Guy; Vianna-Morgante, Angela M; Opitz, John M; Passos-Bueno, Maria Rita

    2005-12-15

    FG syndrome is an X-linked multiple congenital anomalies (MCA) syndrome. It has been mapped to four distinct loci FGS1-4, through linkage analysis (Xq13, Xp22.3, and Xp11.4-p11.3) and based on the breakpoints of an X chromosome inversion (Xq11:Xq28), but so far no gene has been identified. We describe a boy with FG syndrome who has an inherited duplication at band Xq22.3 detected by comparative genomic hybridization microarray (Array-CGH). These duplication maps outside all four loci described so far for FG syndrome, representing therefore a new locus, which we propose to be called FGS5. MID2, a gene closely related to MID1, which is known to be mutated in Opitz G/BBB syndrome, maps within the duplicated segment of our patient. Since FG and Opitz G/BBB syndromes share many manifestations we considered MID2 a candidate gene for FG syndrome. We also discuss the involvement of other potential genes within the duplicated segment and its relationship with clinical symptoms of our patient, as well as the laboratory abnormalities found in his mother, a carrier of the duplication.

  14. Concurrent Supermassive Black Hole and Galazy Growth: Linking Environment and Nuclear Activity in Zeta Equals 2.23 H Alpha Emitters

    Science.gov (United States)

    Lehmer, B. D.; Lucy, A. B.; Alexander, D. M.; Best, P. N.; Geach, J. E.; Harrison, C. M.; Hornschemeier, A. E.; Matsuda, Y.; Mullaney, J. R.; Smail, Ian; Sobral, D.; Swinbank, A. M.

    2013-01-01

    We present results from an approximately equal 100 ks Chandra observation of the 2QZ Cluster 1004+00 structure at z = 2.23 (hereafter 2QZ Clus). 2QZ Clus was originally identified as an overdensity of four optically-selected QSOs at z = 2.23 within a 15 × 15 arcmin square region. Narrow-band imaging in the near-IR (within the K band) revealed that the structure contains an additional overdensity of 22 z = 2.23 H alpha-emitting galaxies (HAEs), resulting in 23 unique z = 2.23 HAEs/QSOs (22 within the Chandra field of view). Our Chandra observations reveal that three HAEs in addition to the four QSOs harbor powerfully accreting supermassive black holes (SMBHs), with 2-10 keV luminosities of approximately equal (8-60) × 10(exp 43) erg s(exp-1) and X-ray spectral slopes consistent with unobscured active galactic nucleus (AGN). Using a large comparison sample of 210 z = 2.23 HAEs in the Chandra-COSMOS field (C-COSMOS), we find suggestive evidence that the AGN fraction increases with local HAE galaxy density. The 2QZ Clus HAEs reside in a moderately overdense environment (a factor of approximately equal 2 times over the field), and after excluding optically-selected QSOs, we find that the AGN fraction is a factor of approximately equal 3.5(+3.8/ -2.2) times higher than C-COSMOS HAEs in similar environments. Using stacking analyses of the Chandra data and Herschel SPIRE observations at 250micrometers, we respectively estimate mean SMBH accretion rates ( M(BH)) and star formation rates (SFRs) for the 2QZ Clus and C-COSMOS samples. We find that the mean 2QZ Clus HAE stacked X-ray luminosity is QSO-like (L(2-10 keV) approximately equal [6-10] × 10(exp 43) erg s(exp -1)), and the implied M(BH)/SFR approximately equal (1.6-3.2) × 10(exp -3) is broadly consistent with the local M(BH)/Stellar Mass relation and z approximately equal 2 X-ray selected AGN. In contrast, the C-COSMOS HAEs are on average an order of magnitude less X-ray luminous and have M(BH)/SFR approximately

  15. Study on the leptin receptor gene Gln223Arg polymorphism in patients with chronic obstructive pulmonary disease accompanied by malnutrition%慢性阻塞性肺疾病伴营养不良患者瘦素受体基因Gln223Arg多态性研究

    Institute of Scientific and Technical Information of China (English)

    陈鹤峰; 李向阳; 缪应新; 甘洁民; 洪慰麟; 周瑾

    2012-01-01

    目的 探讨瘦素受体基因Gln223Arg多态性与慢性阻塞性肺疾病伴营养不良的关系.方法 观察158例COPD临床稳定期老年患者及108例健康对照者,并根据体重指数(BMI)、理想体重百分比(NW%)、三头肌皮皱厚度(TSF)、上臂中点臂围(MAC)、血清白蛋白(ALB)、总淋巴细胞(LYM)等营养参数,将COPD组分为营养不良组(COPD1组)66例,COPD非营养不良组(COPD2组)92例.用酶联免疫吸附试验(ELISA)法测定血清瘦素水平,采用聚合酶链式反应及连接酶检测反应方法(PCR-LDR)测定158例COPD患者与108例对照组的瘦素受体基因Gln223Arg多态性的基因型.结果 COPD营养不良组Gln223Arg基因型GG、GA及从的频率分别为0.924、0.061和0.015,G和A等位基因频率分别为0.955和0.045; COPD非营养不良组Gln223Arg基因型GG、GA及AA的频率分别为0.783、0.206和0.011,G和A等位基因频率分别为0.886和0.114;对照组Gln223Arg基因型GG、GA及AA的频率分别为0.769、0.222和0.009,G和A等位基因频率分别0.88和0.12; COPD 1组Gln223Arg基因型及等位基因频率与COPD2组和对照组比较差异有显著性;COPD 2组和对照组比较差异无显著性.不同基因表型血清瘦素水平GG型低于A/G型+AA型(40.08+17.53 ng/mL vs 44.35±16.95 ng/mL),但差异无统计学意义.结论 瘦素受体基因Gln223Arg多态性可能与COPD营养不良有关.%Objective To investigate the relationship between leptin receptor gene Gln223Arg polymorphism and malnutrition of patients with chronic obstructive pulmonary disease (COPD). Methods 158 elderly patients wilh COPD in stable stage and 10S normal controls were studied. Nutritional parameters, including body mass index (BMI), percentage of normal body mass (NM%), triceps skin-fold thickness (TSF), mid-upper arm circumference (MAC), serum album (ALB), total lymphocyte counts (LYM) in all cases, were evaluated, respectively. COPD patients were divided into malnutrition group (group 1) and non

  16. De-novo 'pure' partial trisomy (6)(p22.3→pter): a case report and review of the literature.

    Science.gov (United States)

    Sivasankaran, Aswini; Murthy, Kanakavalli; Oruganti, Venkata P; Deenadayalu, Anuradha; R Samuel, Chandra; Kandukuri, Lakshmi R

    2017-01-01

    Partial trisomy of the short arm of chromosome 6 is a rare and clinically distinct syndrome. The breakpoints have been found to be variable ranging from bands 6p11 to 6p25. This study reports partial trisomy for 6p22.3→pter in a 2-year-old boy referred with a complaint of developmental delay and facial dysmorphism. Conventional cytogenetic analysis showed the presence of an abnormal chromosome 5 resulting from an unbalanced translocation in the proband. Array comparative genomic hybridization revealed trisomy of distal 6p which was confirmed by fluorescence in situ hybridization using subtelomeric probes for chromosomes 5 and 6. A comparison of the phenotypic features in similar cases of trisomy for different segments of 6p will facilitate an accurate karyotype-phenotype correlation and, subsequently, in the identification of the candidate genes through molecular characterization of the potential genes mapped to these loci.

  17. ONKALO pose experiment. Geophysical logging and imaging of drillholes ONK-PP223, ONK-PP226, ONK-PP254 and ONK-PP259...261

    Energy Technology Data Exchange (ETDEWEB)

    Tarvainen, A.-M. (Suomen Malmi Oy, Espoo (Finland))

    2011-08-15

    Suomen Malmi Oy conducted geophysical drillhole logging as well as optical and acoustic imaging of shallow drillholes ONK-PP223, ONK-PP226, ONK-PP254, ONKPP259, ONK-PP260 and ONK-PP261 at ONKALO in the investigation niche ONKTKU- 3 (POSE) between December 2009 and June 2010. The survey is a part of Posiva Oy's detailed investigation program for the final disposal of spent nuclear fuel. The assignment included the field work and data processing. The report describes field operation, equipment as well as processing procedures and shows the obtained results and an analysis of their quality in the appendices. The raw and processed data are delivered digitally in WellCAD, PDF and Excel format. (orig.)

  18. Thermodynamic and Spectroscopic Investigation of Interactions between Reactive Red 223 and Reactive Orange 122 Anionic Dyes and Cetyltrimethyl Ammonium Bromide (CTAB Cationic Surfactant in Aqueous Solution

    Directory of Open Access Journals (Sweden)

    Muhammad Irfan

    2014-01-01

    Full Text Available The present study describes the conductometric and spectroscopic study of the interaction of reactive anionic dyes, namely, reactive red 223 and reactive orange 122 with the cationic surfactant cetyltrimethyl ammonium bromide (CTAB. In a systematic investigation, the electrical conductivity data was used to calculate various thermodynamic parameters such as free energy (ΔG, enthalpy (ΔH, and the entropy (ΔS of solubilization. The trend of change in these thermodynamic quantities indicates toward the entropy driven solubilization process. Moreover, the results from spectroscopic data reveal high degree of solubilization, with strong interactions observed in the cases of both dyes and the CTAB. The spontaneous nature of solubilization and binding was evident from the observed negative values of free energies (ΔGp and ΔGb.

  19. Thermodynamic and spectroscopic investigation of interactions between reactive red 223 and reactive orange 122 anionic dyes and cetyltrimethyl ammonium bromide (CTAB) cationic surfactant in aqueous solution.

    Science.gov (United States)

    Irfan, Muhammad; Usman, Muhammad; Mansha, Asim; Rasool, Nasir; Ibrahim, Muhammad; Rana, Usman Ali; Siddiq, Mohammad; Zia-Ul-Haq, Muhammad; Jaafar, Hawa Z E; Khan, Salah Ud-Din

    2014-01-01

    The present study describes the conductometric and spectroscopic study of the interaction of reactive anionic dyes, namely, reactive red 223 and reactive orange 122 with the cationic surfactant cetyltrimethyl ammonium bromide (CTAB). In a systematic investigation, the electrical conductivity data was used to calculate various thermodynamic parameters such as free energy (ΔG), enthalpy (ΔH), and the entropy (ΔS) of solubilization. The trend of change in these thermodynamic quantities indicates toward the entropy driven solubilization process. Moreover, the results from spectroscopic data reveal high degree of solubilization, with strong interactions observed in the cases of both dyes and the CTAB. The spontaneous nature of solubilization and binding was evident from the observed negative values of free energies (ΔG p and ΔG b).

  20. Meta-analysis on the relationship between leptin receptor Gln223Arg and Prol019Pro gene polymorphism and obesity in the Chinese population%中国人群瘦素受体Gln223Arg、Pr01019Pro基因多态性与肥胖关联性的Meta分析

    Institute of Scientific and Technical Information of China (English)

    阳赣萍; 彭司华; 左双燕; 王一任; 彭小宁; 曾小敏

    2011-01-01

    目的 探讨瘦素受体(LEPR) Gln223Arg、Prol019Pro基因多态性与肥胖的关联性.方法 计算机检索万方、CNKI、维普、CBM、PubMed、EMBASE数据库,收集1979-2010年公开发表的关于中国人群LEPR Gln223Arg、LEPR Pro1 019Pro基因多态性与肥胖的病例对照研究的文献,选择OR值及其95%CI作为Meta分析指标.利用Stata 10.0软件对各研究结果进行异质性检验和效应值合并计算.结果 根据统一的纳入和剔除标准,纳入15篇文献,其中LEPRGln223Arg基因多态性相关文献9篇,共有肥胖者1096例,对照组949人;LEPR Prol019Pro基因多态性相关文献8篇,共有肥胖者961例,对照组818人.LEPR Gln223Arg基因多态性与肥胖关联性的研究中,LEPR-668位点基因G/A的OR=0.66(95%CI:0.49~0.89),将有A→3基因突变的AG基因型和GG基因型合并后与AA基因型比较,肥胖易感性降低(OR=0.50,95%CI:0.32~0.77)有统计学意义;在LEPR Prol019Pro基因多态性与肥胖关联性的研究中,LEPR-3057位点基因A/G的OR=1,61 (95%CI:1.15~2.26),有G→A基因突变的基因型AG和基因型AA合并后与GG基因型比较,肥胖易感性升高(OR=1.50,95%CI:1.08~2.08),有统计学意义.结论 中国汉族为主的人群LEPR Gln223Arg和LEPR Prol019Pro基因多态性与肥胖的发生均有关联.%Objective To evaluate the associations between polymorphisms of LEPR Gln223Arg,LEPR Pro 1019Pro and the risk on obesity.Methods A computerized search on literature was carried out in Wanfang,CNKI,VIP databases and CBM,PubMed,EMBASE databases to collect articles published between 1979 and 2010 concerning the associations between polymorphisms of LEPR Gln223Arg and/or LEPR Pro 1019Pro and risk of obesity in the Chinese population.Odds ratios (ORs) were used to assess the strength of the association,and 95% confidence intervals (CIs) to present the precision of the estimates.Meta-analysis was performed using the STATA statistical software.Results Fifteen literature

  1. meta Analysis of Effect of LEPR Gln223Arg Polymorphism on Breast Cancer Risk in Different Ethnic Populations%LEPR Gln223Arg多态性对不同种族人群中乳腺癌危险性影响的meta分析

    Institute of Scientific and Technical Information of China (English)

    许晔琼; 李瑞; 高天翼; 顾玲; 聂珍琳; 陈丽萍; 宋国齐; 王书奎

    2012-01-01

    瘦素和瘦素受体参与了乳腺癌的发生发展过程.在瘦素受体基因(LEPR)6号外显子上第223个密码子A到G的转变可以导致谷氨酸到精氨酸的替换(Gln223Arg).许多已发表的病例对照研究评价了LEPR Gln223Arg多态性与乳腺癌的关系.然而,却未得出一致的结论.本篇meta分析囊括了8篇文献来评价LEPR Gln223Arg多态性与乳腺癌的联系.用总体合并OR值作为研究共显性模型、隐性模型、显性模型的指标.结果显示总体研究中隐性模型(OR=1.32,95%CI:1.03~1.69)和Arg/Gln vs Gln/Gln基因型(OR=1.16,95%CI:1.01~1.34)显著提高了乳腺癌的危险性.种族分层分析中发现,非洲人群的以下几个基因型会提高患乳腺癌的危险性:Arg/Arg vs Gln/Gln (OR=1.86,95%CI:1.28~2.71),Arg/Gln vs Gln/Gln (OR=1.48,95%CI:1.10~1.99),显性模型(OR=1.60,95%CI:1.21~2.11)和隐性模型(OR=1.48,95%CI:1.07~2.05);亚洲人群中,Arg/Arg vs Gln/Gln基因型(OR=6.79,95%CI:3.42~13.47)和显性模型(OR=2.03,95%CI:1.42~2.90)提高了患乳腺癌的危险性.在欧洲人群中任何基因模型都没有发现能显著提高乳腺癌的危险性.总而言之,LEPR 223Arg是乳腺癌发展的低风险因素,特别是在非洲女性中.%Leptin and leptin receptor have been implicated in processes of breast cancer. An A to G transition of codon 223th in exon 6 of leptin receptor gene (LEPR) can result in the substitution from glutamine to arginine (Gln223Arg). A variety of case-control papers have been published to evaluate the association between LEPR Gln223Arg polymorphism and breast cancer. However, they have got contradictory conclusions. This meta analysis enrolls 8 papers to evaluate the connection of them. The pooled Ors are took for codominant model, recessive model, dominant model. The result shows that recessive model (OR=1.32, 95%CI: 1.03-1.69) and genotype Arg/Gln vs Gln/Gln (OR=1.16, 95%CI: 1.01-1.34) significantly improve breast cancer risk

  2. Discovery and Monitoring of a New Black Hole Candidate XTE J1752-223 with RXTE: RMS Spectrum Evolution, BH Mass and the Source Distance

    Science.gov (United States)

    Shaposhinikov, Nikolai; Markwardt, Craig; Swank, Jean; Krimm, Hans

    2010-01-01

    We report on the discovery and monitoring observations of a new galactic black hole candidate XTE J1752-223 by Rossi X-ray Timing Explorer (RXTE). The new source appeared on the X-ray sky on October 21 2009 and was active for almost 8 months. Phenomenologically, the source exhibited the low-hard/highsoft spectral state bi-modality and the variability evolution during the state transition that matches standard behavior expected from a stellar mass black hole binary. We model the energy spectrum throughout the outburst using a generic Comptonization model assuming that part of the input soft radiation in the form of a black body spectrum gets reprocessed in the Comptonizing medium. We follow the evolution of fractional root-mean-square (RMS) variability in the RXTE/PCA energy band with the source spectral state and conclude that broad band variability is strongly correlated with the source hardness (or Comptonized fraction). We follow changes in the energy distribution of rms variability during the low-hard state and the state transition and find further evidence that variable emission is strongly concentrated in the power-law spectral component. We discuss the implication of our results to the Comptonization regimes during different spectral states. Correlations of spectral and variability properties provide measurements of the BH mass and distance to the source. The spectral-timing correlation scaling technique applied to the RXTE observations during the hardto- soft state transition indicates a mass of the BH in XTE J1752-223 between 8 and 11 solar masses and a distance to the source about 3.5 kiloparsec.

  3. Myofibrillar myopathy with arrhythmogenic right ventricular cardiomyopathy 7: corroboration and narrowing of the critical region on 10q22.3.

    Science.gov (United States)

    Kuhl, Angelika; Melberg, Atle; Meinl, Edgar; Nürnberg, Gudrun; Nürnberg, Peter; Kehrer-Sawatzki, Hildegard; Jenne, Dieter E

    2008-03-01

    Several years ago, autosomal dominant myofibrillar myopathy (MFM) in combination with arrhythmogenic right ventricular cardiomyopathy (ARVC7) was tentatively mapped to a 10.6-Mbp (million base pairs) region on chromosome 10q22.3 between D10S605 (78.9 Mbp) and D10S215 (89.5 Mbp) in a Swedish family assuming that ARVC7 was allelic with cardiomyopathy, dilated 1C (CMD1C). To date, neither the genetic defect in ARVC7 nor CMD1C has been reported. In a comprehensive follow-up study we re-examined and confirmed the previous linkage data for ARVC7 using a high-density single nucleotide polymorphism marker panel from Affymetrix (Human Mapping 10K Array). No other regions with significant evidence for linkage were discovered. The critical interval was narrowed down to 4.27 Mbp between D10S1645 and D10S1786. This reduced the total number of candidate genes to 18 of which 17 (RAI17, PPIF, C10ORF56, SFTPA1, SFTPA2, SFTPA1B, SFTPA2B, SFTPD, C10ORF57, PLAC9, ANXA11, MAT1A, DYDC1, DYDC2, C10ORF58, TSPAN14 and SH2D4B) are shared with the CMD1C region. No disease-causing mutation was found in their coding regions. Moreover, metavinculin (VCL) and ZASP/cypher (LDB3) proximal and distal to this linked region were excluded by sequence analysis. To search for submicroscopic and intragenic deletions by PCR, we generated hybrid cell lines carrying only the affected or normal chromosome 10 homolog. All sequence tagged sites and exons were present on both homologs. We speculate that regulatory mutations in 1 of the 18 genes from 10q22.3 are responsible for a heterogenous spectrum of clinically distinct myodegenerative disorders, affecting both skeletal and cardiac muscles to variable degrees.

  4. 228Ra, 226Ra, 224Ra and 223Ra in potential sources and sinks of land-derived material in the German Bight of the North Sea: implications for the use of radium as a tracer

    NARCIS (Netherlands)

    Schmidt, C.; Hanfland, C.; Regnier, P.; Van Cappellen, P.; Schlüter, M.; Knauthe, U.; Stimac, I.; Geibert, W.

    2011-01-01

    Activities of the naturally occurring radium nuclides 228Ra, 226Ra, 224Ra and 223Ra were determined in waters of the open German Bight and adjacent nearshore areas in the North Sea, in order to explore the potential use of radium isotopes as natural tracers of land–ocean interaction in an environmen

  5. Evaluation of Alpha-Therapy with Radium-223-Dichloride in Castration Resistant Metastatic Prostate Cancer—the Role of Gamma Scintigraphy in Dosimetry and Pharmacokinetics

    Directory of Open Access Journals (Sweden)

    Kalevi Kairemo

    2015-07-01

    Full Text Available Radium-223-dichloride (223RaCl2 is a new bone-seeking calcium analogue alpha-emitter, which has obtained marketing authorization for the treatment skeletal metastases of hormone-refractory prostate cancer. The current treatment regimen is based on six consecutive doses of 223RaCl2 at 4 week intervals and the administered activity dose, 50 kBq/kg per cycle is based on patient weight. We analyzed two patients using quantitative serial gamma imaging to estimate dosimetry in tumors and see possible pharmacokinetic differences in the treatment cycles. The lesions were rather well visualized in gamma scintigraphy in spite of low gamma activity (<1.1% gamma radiation at 0, 7 and 28 days using 30–60 min acquisition times. Both our patients analyzed in serial gamma imagings, had two lesions in the gamma imaging field, the mean counts of the relative intensity varied from 27.8 to 36.5 (patient 1, and from 37.4 to 82.2 (patient 2. The half-lives varied from 1.8 days to 4.5 days during the six cycles (patient 1, and from 1.5 days to 3.6 days (patient 2, respectively. In the lesion half-lives calculated from the imaging the maximum difference between the treatment cycles in the same lesion was 2.0-fold (1.8 vs. 3.6. Of these patients, patient 1 demonstrated a serum PSA response, whereas there was no PSA response in patient 2. From our data, there were maximally up to 4.0-fold differences (62.1 vs. 246.6 between the relative absorbed radiation doses between patients as calculated from the quantitative standardized imaging to be delivered in only two lesions, and in the same lesion the maximum difference in the cycles was up to 2.3-fold (107.4 vs. 246.6. Our recommendation based on statistical simulation analysis, is serial measurement at days 0–8 at least 3 times, this improve the accuracy significantly to study the lesion activities, half-lives or calculated relative absorbed radiation doses as calculated from the imaging. Both our patients had

  6. Tectonic Influence on Magma Storage and Ascent During the Older Evolutionary Stages (223-105 ka) of the Lipari Island (Aeolian Archipelago, Southern Italy)

    Science.gov (United States)

    di Martino, C.; Frezzotti, M.; Lucchi, F.; Peccerillo, A.; Tranne, C.; Diamond, L.

    2008-12-01

    Pure CO2 fluid inclusions are observed in fifteen quartz-rich xenoliths collected in basaltic-andesitic to andesitic volcanic products relevant to the older evolutionary stages of Lipari Island (223-105 ka). In volcanics forming central composite volcanoes (M. Mazzacaruso, 223-127 ka; M. S.Angelo, 105 ka), fluid inclusions are trapped during two distinct events: early Type I inclusions formed before host magma transport, and late (i.e. secondary) Type II inclusions trapped during magma ascent. Early Type I inclusions show homogenization temperatures corresponding to densities from 0.9 to 0.6 g/cc, while Type II inclusions record a considerably lower density interval between 0.38 and 0.1 g/cc. At the estimated trapping temperatures between 950 and 1090°C, obtained density values correspond to pressures of 0.58- 0.25 GPa (22-10 km) for Type I, and 0.13-0.03 GPa (5.5-1 km) for Type II inclusions, respectively. In those magmas erupted from fissural eruptive vents aligned along the main regional NNW-SSE and E-W faults systems (Timpone Ospedale, Monterosa and M. Chirica; 223-127 ka) only early Type I inclusions are observed. Density values form to two distinct intervals between 0.87 and 0.6 g/cc (0.53-0.25 GPa; 20-10 km; M. Chirica), and between 0.68-0.18 g/cc (0.32-0.05 GPa; 12-2 km; Timpone Ospedale and Monterosa). Fluid inclusion data together with tectonic features outline a complex magma storage and ascent evolution during the Lipari's older evolutionary stages. Beneath the central volcanoes of M. Mazzacaruso, M. S.Angelo and the M. Chirica, two magma reservoirs, located at lower crustal depths (~22 km; close to the Moho) and at very shallow levels (5.5-1 km), are present. Mantle-Derived magmas are accumulated into the deep magma chamber and may then reside in the shallower reservoir for a short period of time before being erupted to the surface. Such a magma feeding system is similar to those outlined for the Alicudi and Stromboli volcanoes, and for most of the

  7. Response of MiRNA-22-3p and MiRNA-149-5p to Folate Deficiency and the Differential Regulation of MTHFR Expression in Normal and Cancerous Human Hepatocytes

    Science.gov (United States)

    Li, Chao; Ni, Juan; Liu, Yao-Xian; Wang, Han; Liang, Zi-Qing; Wang, Xu

    2017-01-01

    Background/Aims Folic acid (FA) is a core micronutrient involved in DNA synthesis/methylation, and the metabolism of FA is responsible for genomic stability. MicroRNAs may affect gene expression during folate metabolism when cellular homeostasis is changed. This study aimed to reveal the relationship between FA deficiency and the expression of miR-22-p/miR-149-5p and the targeted regulation of miR-22-3p/miR-149-5p on the key folate metabolic gene Methylenetetrahydrofolate reductase (MTHFR). Methods Normal (HL-7702 cells) and cancerous (QGY-7703 cells) human hepatocytes were intervened in modified RPMI 1640 with FA deficiency for 21 days. The interaction between MTHFR and the tested miRNAs was verified by Dual-Luciferase Reporter Assays. The changes in the expression of miR-22-3p/miR-149-5p in response to FA deficiency were detected by Poly (A) Tailing RT-qPCR, and the expression of MTHFR at both the transcriptional and translational levels was determined by RT-qPCR and Western blotting, respectively. Result MiR-22-3p/miR-149-5p directly targeted the 3’UTR sequence of the MTHFR gene. FA deficiency led to an upregulation of miR-22-3p/miR-149-5p expression in QGY-7703/HL-7702 cells, while the transcription of MTHFR was decreased in QGY-7703 cells but elevated in HL-7702 cells. Western blotting showed that FA deficiency resulted in a decline of the MTHFR protein in QGY-7703 cells, whereas in HL-7702 cells, the MTHFR protein level remained constant. Conclusion The results suggested that miR-22-3p/miR-149-5p exert different post-transcriptional effects on MTHFR under conditions of FA deficiency in normal and cancerous human hepatocytes. The results also implied that miR-22-3p/miR-149-5p might exert anticancer effects in cases of long-term FA deficiency. PMID:28045918

  8. The CALYMHA survey: Lyα luminosity function and global escape fraction of Lyα photons at z = 2.23

    Science.gov (United States)

    Sobral, David; Matthee, Jorryt; Best, Philip; Stroe, Andra; Röttgering, Huub; Oteo, Iván; Smail, Ian; Morabito, Leah; Paulino-Afonso, Ana

    2017-04-01

    We present the CAlibrating LYMan-α with Hα (CALYMHA) pilot survey and new results on Lyman α (Lyα) selected galaxies at z ∼ 2. We use a custom-built Lyα narrow-band filter at the Isaac Newton Telescope, designed to provide a matched volume coverage to the z = 2.23 Hα HiZELS survey. Here, we present the first results for the COSMOS and UDS fields. Our survey currently reaches a 3σ line flux limit of ∼4 × 10-17 erg s-1 cm-2, and a Lyα luminosity limit of ∼1042.3 erg s-1. We find 188 Lyα emitters over 7.3 × 105 Mpc3, but also find significant numbers of other line-emitting sources corresponding to He II, C III] and C IV emission lines. These sources are important contaminants, and we carefully remove them, unlike most previous studies. We find that the Lyα luminosity function at z = 2.23 is very well described by a Schechter function up to LLy α ≈ 1043 erg s-1 with L^{ast }=10^{42.59^{+0.16}_{-0.08}} erg s-1, φ ^{ast }=10^{-3.09^{+0.14}_{-0.34}} Mpc-3 and α = -1.75 ± 0.25. Above LLy α ≈ 1043 erg s-1, the Lyα luminosity function becomes power-law like, driven by X-ray AGN. We find that Lyα-selected emitters have a high escape fraction of 37 ± 7 per cent, anticorrelated with Lyα luminosity and correlated with Lyα equivalent width. Lyα emitters have ubiquitous large (≈40 kpc) Lyα haloes, ∼2 times larger than their Hα extents. By directly comparing our Lyα and Hα luminosity functions, we find that the global/overall escape fraction of Lyα photons (within a 13 kpc radius) from the full population of star-forming galaxies is 5.1 ± 0.2 per cent at the peak of the star formation history. An extra 3.3 ± 0.3 per cent of Lyα photons likely still escape, but at larger radii.

  9. Holoprosencephaly: Characterization of the deletion of 21q22.3 and isolation of cDNAs by a direct selection technique

    Energy Technology Data Exchange (ETDEWEB)

    Yamakawa, K.; Colbern, S.; Brusilovsky, A. [Univ. of California, Los Angeles, CA (United States)] [and others

    1994-09-01

    Holoprosencephaly (HP) is characterized by impaired cleavage of the embryonic forebrain and incomplete mid-facial development. The etiology is heterogeneous and may include aneuploidies for chromosomes 2, 3, 7, 13, 18 and 21. We have narrowed the chromosome 21 candidate region by analyzing 2 cases of HP with deletion 21q22 using FISH and Southern blot techniques. For the smaller deletion, the regions for D21S25, D21S154, D21S171 and D21S44 were deleted and for D21S42 was not. Combining these data with previous reports of deletion of 21q22.3 (ColVIA2-ter) without the holoprosencephaly phenotype indicate that the region responsible for holoprosencephaly spans the 2-3 Mb region including PFKL and ITGB2 (CD18) that has also been linked to progressive myoclonus epilepsy (EPM1). In order to isolate genes responsible for these diseases, we constructed a cDNA library from a 14-week trisomy 21 fetal brain using Uni-Zap XR (Stratagene). More than 95% clones have inserts ranging from 1-4 kb (ave. 2 kb). In addition we applied a direct cDNA selection method to BACs (Bacterial Artificial Chromosomes) in the 21q22.3 region. Using cDNA synthesized from trisomy 21 fetal brain, we attached Sau3AI linkers, digested with Sau3AI, attached second linkers and hybridized to biotinylated BAC DNAs which cover the candidate region. cDNA/BAC DNA hybrid molecules were captured on streptavidin-coated magnetic beads, non-specific cDNA were washed out, and specifically hybridized cDNA were eluted and amplified by PCR. Twice-selected PCR products were subcloned and analyzed. Southern blot analyses revealed that 21 out of 30 (70%) of fragments yielded unique bands on the original BACs. Eight clones contained repetitive sequences. We are now isolating cDNAs expressed in the Down syndrome fetal brain using these cDNA fragments. These genes now provide candidates for EPM1 and holoprosencephaly.

  10. Crystalline structure of N-(S)-2-heptyl (1R,2R)-2-(2,3-anthracenedicarboximido)cyclohexanecarboxamide that differs from its preferred conformation in the solvent used for crystallization.

    Science.gov (United States)

    Akasaka, Kazuaki; Ohtaki, Takashi; Kabuto, Chizuko; Kitahara, Takeshi; Ohrui, Hiroshi

    2005-10-01

    The crystalline structure of N-(S)-2-heptyl (1R,2R)-2-(2,3-anthracenedicarboximido)cyclohexamide (1), which was crystallized from methanol, was determined by an X-ray analysis and had a different conformation from its preferred one in CD3OD by a 1H-NMR analysis. Inter- and intra-molecular CH-pi interaction in a crystal plays a very important role in crystal packing. The preferred conformation of the amide derivative in a solution allows us to exploit (1R,2R)-2-(2,3-anthracenedicarboximido)cyclohexanecarbonyl chloride as a conversion reagent to determine the absolute configuration of chiral amines by 1H-NMR.

  11. Inherited and de novo deletion of the tyrosine aminotransferase gene locus at 16q22.1----q22.3 in a patient with tyrosinemia type II.

    Science.gov (United States)

    Natt, E; Westphal, E M; Toth-Fejel, S E; Magenis, R E; Buist, N R; Rettenmeier, R; Scherer, G

    1987-12-01

    Tyrosinemia II is an autosomal-recessively inherited condition caused by deficiency in the liver-specific enzyme tyrosine aminotransferase (TAT; EC 2.6.1.5). We have restudied a patient with typical symptoms of tyrosinemia II who in addition suffers from multiple congenital anomalies including severe mental retardation. Southern blot analysis using a human TAT cDNA probe revealed a complete deletion of both TAT alleles in the patient. Molecular and cytogenetic analysis of the patient and his family showed one deletion to be maternally inherited, extending over at least 27 kb and including the complete TAT structural gene, whereas loss of the second TAT allele results from a small de novo interstitial deletion, del 16 (pter----q22.1::q22.3----qter), in the paternally inherited chromosome 16. Three additional loci previously assigned to 16q22 were studied in our patient: haptoglobin (HP), lecithin: cholesterol acyltransferase (LCAT), and the metallothionein gene cluster MT1,MT2. Of these three markers, only the HP locus was found to be codeleted with the TAT locus on the del(16) chromosome.

  12. Cloning of the cDNA for a human homologue of the Drosophila white gene and mapping to chromosome 21q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Haiming Chen; Lalioti, M.D.; Perrin, G.; Antonarakis, S.E. [Univ. of Geneva Medical School (Switzerland)] [and others

    1996-07-01

    In an effort to contribute to the transcript map of human chromosome 21 and the understanding of the pathophysiology of trisomy 21, we have used exon trapping to identify fragments of chromosome 21 genes. Two trapped exons, from pools of chromosome 21-specific cosmids, showed homology to the Drosophila white (w) gene. We subsequently cloned the corresponding cDNA for a human homologue of the Drosophila w gene (hW) from human retina and fetal brain cDNA libraries. The gene belongs to the ATP-binding cassette transporter gene family and is homologous to Drosophila w (and to 2 genes from other species) and to a lesser extent to Drosophila brown (bw) and scarlet (st) genes that are all involved in the transport of eye pigment precursor molecules. A DNA polymorphism with 62% heterozygosity due to variation of a poly (T) region in the 3{prime} UTR of the hW has been identified and used for the incorporation of this gene to the genetic map of chromosome 21. The hW is located at 21q22.3 between DNA markers D21S212 and D21S49 in a P1 clone that also contains marker BCEI. The gene is expressed at various levels in many human tissues. The contributions of this gene to the Down syndrome phenotypes, to human eye color, and to the resulting phenotypes of null or missense mutations are presently unknown. 56 refs., 8 figs., 1 tab.

  13. Fructose-1,6-bisphosphatase: Genetic and physical mapping to human chromosome 9q22.3 and evaluation in non-insulin-dependent diabetes mellitus

    Energy Technology Data Exchange (ETDEWEB)

    Rothschild, C.B.; Akots, G.; Roh, B. [Wake Forest Univ., Winston-Salem, NC (United States)] [and others

    1995-09-01

    PCR primers specific to the human liver fructose-1,6-bisphosphatase (FBP) gene were designed and used to isolate a cosmid clone. Physical mapping of the FBP cosmid by FISH, and genetic mapping of an associated GA repeat polymorphism (PIC = 0.35), located the liver FBP gene to chromosome 9q22.3 with no recombination between FBP and the index markers D9S196 (Z{sub max} = 13.2), D9S280 (Z{sub max} = 11.7), D9S287 (Z{sub max} = 15.6), and D9S176 (Z{sub max} = 14.4). Amplification using FBP exon-specific primers with a YAC contig from this region of chromosome 9 further refined the placement of FBP genomic sequences to an approximately 1.7-cM region flanked by D9S280 and D9S287, near the gene for Fanconi anemia group C. Precise localization of the FBP gene enabled evaluation of FBP as a candidate gene for maturity-onset diabetes of the young (MODY) and non-insulin-dependent diabetes (NIDDM) in both Caucasian and African-American families, using the highly informative markers D9S287 and D9S176. Although FBP is a rate-limiting enzyme in gluconeogenesis, using both parametric and nonparametric analysis there was no evidence for linkage of FBP to diabetes in these families. 30 refs., 4 figs., 2 tabs.

  14. 3.2 Mb microdeletion in chromosome 7 bands q22.2-q22.3 associated with overgrowth and delayed bone age.

    Science.gov (United States)

    Uliana, Vera; Grosso, Salvatore; Cioni, Maddalena; Ariani, Francesca; Papa, Filomena T; Tamburello, Silvia; Rossi, Elisa; Katzaki, Eleni; Mucciolo, Mafalda; Marozza, Annabella; Pollazzon, Marzia; Mencarelli, Maria Antonietta; Mari, Francesca; Balestri, Paolo; Renieri, Alessandra

    2010-01-01

    We report a patient with mental retardation, epilepsy, overgrowth, delayed bone age, peculiar facial features, corpus callosum hypoplasia, enlarged cisterna magna and right cerebellar hypoplasia. Array-CGH analysis revealed the presence of a de novo 3.2 Mb interstitial deletion of the long arm of chromosome 7 involving bands q22.2-q22.3. The rearrangement includes 15 genes and encompasses a genomic region that represents a site of frequent loss of heterozygosity in myeloid malignancies. Four genes are implicated in the control of cell cycle: SRPK2, MLL5, RINT1 and LHFPL3. Haploinsufficiency of these genes might therefore be associated with overgrowth and could confer susceptibility to cancers or other tumours, so that attention to this possibility would be appropriate during regular medical review. In conclusion, array-CGH analysis should be performed in patients with overgrowth where the known causes have already been excluded, because some still unclassified overgrowth syndromes may be caused by subtle genomic imbalances.

  15. A Spectral Study of the Black Hole Candidate XTE J1752-223 in the High/Soft State with MAXI, Suzaku and Swift

    CERN Document Server

    Nakahira, Satoshi; Uedu, Yoshihiro; Yamaoka, Kazutaka; Sugizaki, Mutsumi; Mihara, Tatehiro; Matsuoka, Masaru; Yoshida, Atsumasa; Makishima, Kazuo; Ebisawa, Ken; Kubota, Aya; Yamada, Shin'ya; Negoro, Hitoshi; Hiroi, Kazuo; Ishikawa, Masaki; Kawai, Nobuyuki; Kimura, Masashi; Kitayama, Hiroki; Kohama, Mitsuhiro; Matsumura, Takanori; Morii, Mikio; Nakajima, Motoki; Serino, Motoko; Shidatsu, Megumi; Sootome, Tetsuya; Sugimori, Kousuke; Suwa, Fumitoshi; Tomida, Hiroshi; Tsuboi, Yoko; Tsunemi, Hiroshi; Ueno, Shiro; Usui, Ryuichi; Yamamoto, Takayuki; Yamazaki, Kyohei; Tashiro, Makoto S; Terada, Yukikatsu; Seta, Hiromi

    2011-01-01

    We report on the X-ray spectral analysis of the black hole candidate XTE\\ J1752--223 in the 2009--2010 outburst, utilizing data obtained with the MAXI/Gas Slit Camera (GSC), the Swift/XRT, and Suzaku, which work complementarily. As already reported by Nakahira et al. (2010) MAXI monitored the source continuously throughout the entire outburst for about eight months. All the MAXI/GSC energy spectra in the high/soft state lasting for 2 months are well represented by a multi-color disk plus power-law model. The innermost disk temperature changed from $\\sim$0.7 keV to $\\sim$0.4 keV and the disk flux decreased by an order of magnitude. Nevertheless, the innermost radius is constant at $\\sim$41 $D_{3.5}(\\cos{\\it i})^{-1/2}$ km, where $D_{3.5}$ is the source distance in units of 3.5 kpc and $i$ the inclination. The multi-color disk parameters obtained with the MAXI/GSC are consistent with those with the Swift/XRT and Suzaku. The Suzaku data also suggests a possibility that the disk emission is slightly Comptonized, ...

  16. MAGMA STORAGE AND ASCENT AT LIPARI ISLAND (AEOLIAN ARCHIPELAGO, SOUTHERN ITALY) DURING THE OLD STAGES (223-81 KA): ROLE OF CRUSTAL PROCESSES AND TECTONIC INFLUENCE

    Science.gov (United States)

    di Martino, C.; Frezzotti, M.; Lucchi, F.; Peccerillo, A.; Tranne, C. A.; Diamond, L.

    2009-12-01

    Fluid inclusion study, together with structural, volcanological and petrochemical data, allow reconstruction of the magma feeding system of basaltic-andesitic to andesitic volcanoes during the oldest and intermediate stages of development of Lipari Island (223-81 ka). A major magma storage zone is active during the overall investigated time span at depths of ~22 km close to the crust-mantle Moho transition, at which mantle-derived mafic magmas tend to accumulate due to neutral buoyancy conditions. A shallow magma reservoir is found at depths of 5.5-3.5 km in correspondence of a major lithological discontinuity within the upper crust beneath central-type volcanoes (M. Mazzacaruso, M. S.Angelo, M. Chirica-Costa d'Agosto). It is the place where magmas shortly rest (day-week time scale) before the eruption. For fissural-type volcanoes (Timpone Ospedale, Monterosa, M. Chirica), tectonic structures are suggested to intercept magma batches at different crustal depths (~14 km), thus provoking CO2-degassing and rapid magma uprising with no substantial ponding at shallow levels. Partial crustal melting processes at roof of the deep magma reservoirs are invoked to explain conditions of fluids trapping at depths of volcanoes, active during the intermediate stages of development of Lipari (105-81 ka). These melting processes determine the generation of felsic melts which may act as a density-barrier and prevent the uprising of mantle-derived magmas, with an influence on the subsequent evolution of the magma feeding system of Lipari.

  17. Magma storage and ascent at Lipari Island (Aeolian archipelago, Southern Italy) at 223-81 ka: the role of crustal processes and tectonic influence

    Science.gov (United States)

    di Martino, Corrado; Frezzotti, Maria Luce; Lucchi, Federico; Peccerillo, Angelo; Tranne, Claudio A.; Diamond, Larryn W.

    2010-11-01

    Fluid inclusion studies together with volcanological and petrochemical data allow reconstruction of the magma feeding system of basaltic-andesitic to andesitic activity during the oldest and intermediate stages of development of Lipari Island (223-81 ka). A major magma storage zone is active during the overall investigated time span at depths of 22 km, close to the crust-mantle Moho transition, at which mantle-derived mafic magmas tend to accumulate due to neutral buoyancy conditions. Beneath central-type volcanoes (M. Mazzacaruso, M. S.Angelo, M. Chirica-Costa d’Agosto), a shallower magma reservoir is located within the upper crust at 5.5-3.5 km, associated with a major lithological discontinuity. For fissural-type volcanoes (Timpone Ospedale, Monterosa, M. Chirica), tectonic structures are suggested to influence further magma ascent and storage at mid-crustal depths (˜14 km), with no ponding at shallower levels. Partial crustal melting processes at the roofs of the deep magma reservoirs (˜17 km) are invoked to explain the origin of cordierite-bearing lavas beneath M. S.Angelo and M. Chirica-Costa d’Agosto volcanoes, which were active during the intermediate stages of development of Lipari (105-81 ka). The generation of felsic anatectic melts in the lower crust could have created density and rheologic barriers to impede the passage of mafic melts and promote their ponding, with influence on the subsequent evolution of Lipari volcano.

  18. Psychiatric disorder in a familial 15;18 translocation and sublocalization of myelin basic protein to 18q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Calzolari, E.; Aiello, V.; Palazzi, P.; Sensi, A. [Universita Ferrara (Italy)] [and others

    1996-04-09

    Two related patients with similar clinical features consisting of a few dysmorphic signs and psychiatric disturbance were reported to have a partial trisomy of chromosomes 15(pter-q13.3) and 18(q23-qter) deriving from a familial translocation t(15;18). One patient is affected by bipolar disorder and the other by schizoaffective disorder. Both cases have a predominantly affective course; nevertheless, a clear diagnosis is difficult in the first patient, who is 15 years of age, and only a longitudinal course will allow us to establish a definite diagnosis. The possibility that these two pathologies belong to a single category is discussed, and the presence of a susceptibility locus on chromosome 18 is hypothesized. Cytogenetic data, FISH, and DNA studies indicate that the myelin basic protein (MPB) gene is not involved in the translocation, and localize it centromeric to the breakpoint on chromosome 18(q22.3). Thus, it is unlikely to be involved in the disease. 58 refs., 8 figs.

  19. Multi-state observations of the Galactic Black Hole XTE J1752-223: Evidence for an intermediate black hole spin

    CERN Document Server

    Reis, R C; Fabian, A C; Cackett, E M; Maitra, D; Reynolds, C S; Rupen, M; Steeghs, D T H; Wijnands, R

    2010-01-01

    The Galactic Black hole candidate XTE J1752-223 was observed during the decay of its 2009 outburst with the Suzaku and XMM-Newton observatories. The observed spectra are consistent with the source being in the ''intermediate`` and ''low-hard state`` respectively. The presence of a strong, relativistic iron emission line is clearly detected in both observations and the line profiles are found to be remarkably consistent and robust to a variety of continuum models. This strongly points to the compact object in \\j\\ being a stellar-mass black hole accretor and not a neutron star. Physically-motivated and self-consistent reflection models for the Fe-\\ka\\ emission-line profile and disk reflection spectrum rule out either a non-rotating, Schwarzchild black hole or a maximally rotating, Kerr black hole at greater than 3sigma level of confidence. Using a fully relativistic line function in which the black hole spin parameter is a variable, we have formally constrained the spin parameter to be $0.52\\pm0.11 (1\\sigma)$. ...

  20. The gene encoding human intestinal trefoil factor (TFF3) is located on chromosome 21q22.3 clustered with other members of the trefoil peptide family

    Energy Technology Data Exchange (ETDEWEB)

    Chinery, R. [Royal College of Surgeons of England, London (United Kingdom); Williamson, J.; Poulsom, R. [Imperial Cancer Research Fund, London (United Kingdom)

    1996-03-01

    The gene coding for human intestinal trefoil factor (hITF), a recently described cellular motogen produced by gastrointestinal goblet cells and epithelia elsewhere, is a member of the rapidly growing trefoil peptide family. In a rodent-human somatic cell hybrid panel, the hITF (HGMW-approved symbol TFF3) genomic locus segregated with human chromosome 21q. Fluorescence in situ hybridization with a 2.1-kb genomic probe of the hITF gene mapped this locus more precisely to the q22.3 region. Triple fluorescence in situ hybridization, together with physical mapping of human genomic DNA using pulsed-field gel electrophoresis, revealed that the hITF gene is tightly linked to those encoding the other known human trefoil peptides, namely the breast cancer estrogen-inducable gene pS2 (BCEI) and human spasmolytic polypeptide (hSP/SML1). This gene family could become a useful marker for the genetic and physical mapping of chromosome 21 and for a better definition of the region involved in the clinical phenotype of several genetic diseases. 17 refs., 2 figs.

  1. ENDOR Spectroscopy Reveals A Light Induced Movement of the H-Bond from Ser-L223 Upon Forming the Semiquinone (QB−•) in Reaction Centers from Rhodobacter sphaeroides

    Science.gov (United States)

    Paddock, M. L.; Flores, M.; Isaacson, R.; Chang, C.; Abresch, E. C.; Okamura, M.Y.

    2008-01-01

    Proton ENDOR spectroscopy was used to monitor local conformational changes in bacterial reaction centers (RC) associated with the electron transfer reaction DQB → D+•QB−• using mutant RCs capable of photo-reducing QB at cryogenic temperatures. The charge separated state D+•QB−• was studied in mutant RCs formed by either (i) illuminating at low temperature (77K) a sample frozen in the dark (ground state protein conformation) or (ii) illuminating at room temperature prior to and during the freezing (charge separated state protein conformation). The charge recombination rates from the two states differed greatly (>106 fold) as shown previously, indicating a structural change (Paddock et al (2006) Biochemistry 45, 14032 - 14042). ENDOR spectra of QB−• from both samples (35 GHz, 77K) showed three nearly identical sets of hyperfine couplings due to exchangeable protons that were similar to those for QB−• in native RCs indicating that in all RCs, QB−• was located at the proximal position near the metal site. In contrast, one set of H-bond couplings was observed only in the sample frozen under illumination in which the protein can relax prior to freezing. This H-bond was assigned to an interaction between the Ser-L223 hydroxyl and QB−• based on its absence in Ser L223 → Ala mutant RCs. The Ser-L223 hydroxyl H-bond was also observed in the native RCs frozen under illumination. Thus, part of the protein relaxation in response to light induced charge separation involves the formation of an H-bond between the OH group of Ser-L223 and the anionic semiquinone QB−•. This proton movement serves to stabilize the charge separated state and facilitate proton transfer to reduced QB. PMID:17590017

  2. SWOT Analysis on LI Ning Sports Brand ---Based on Consumer's Cognition and Behavior of the Generation after 90s of 223 College Students%“李宁”运动服饰品牌的SWOT分析--基于223名90后大学生的体育品牌消费认知和行为分析

    Institute of Scientific and Technical Information of China (English)

    颜耀春; 窦雨佳; 王浩; 王鸣骏

    2014-01-01

    探讨90后年轻人对“李宁”服饰品牌消费认知和消费行为状态,有利于从现实角度厘清国产体育品牌目前处于市场低迷原因。本文采用问卷调查法、访谈法、统计法、逻辑分析法,以223名被调查者问卷统计数值为依据,结合部分消费者访谈的资料,分析出“李宁”服饰作为国产品牌在90后消费者中具有较高认知度,他们对“李宁”的消费属于品质和时尚结合型,而目前“李宁”的产品在时尚元素和产品科技水平等方面,距离90后消费者要求存在距离。建议“李宁”团队在产品设计上更多研究90后消费需要和审美心理,同时建议应和国外品牌差异化发展。%To analyze consumer's cognition and behavior of the generation after 90s to LI Ning sports brand is to find the reason why the market share of domestic-made sports brand is low.With questionnaire, statistics and logical analysis, based on the questionnaire of 223 College Students, this paper concludes that LI Ning sports brand has a higher visibility because it combines good quality and fashion.But LI Ning sports brand still cannot meet the needs of the generation after 90s on fashion and high-tech.It is suggested that LI Ning sports brand should do more research on consumer's needs and aesthetics and seek differentiation development strategy from foreign brands.

  3. Health Economics and Radium-223 (Xofigo®) in the Treatment of Metastatic Castration-Resistant Prostate Cancer (mCRPC): A Case History and a Systematic Review of the Literature

    OpenAIRE

    Norum, Jan; Traasdahl, Erik R.; Totth, Arpad; Nieder, Carsten; Olsen, Jan Abel

    2015-01-01

    DOI: 10.5539/gjhs.v8n4p1 Creative Commons Attribution License 3.0 OBJECTIVES: Prostate cancer (PC) is the most common cancer in Western countries. Recent advances in the treatment of metastatic castration resistant prostate cancer (mCRPC) have caused significant pressure on health care budgets. We aimed to exemplify this dilemma presenting an example, radium-223 (Xofigo®), and review the literature. METHODS: A 74-year-old man diagnosed with mCRPC was referred to our department in O...

  4. catena-Poly[[bis[2-(2,3-dimethylanilinobenzoato-κO]cadmium(II]-di-μ-3-pyridylmethanol-κ2N:O;κ2O:N

    Directory of Open Access Journals (Sweden)

    Tadeusz Lis

    2008-03-01

    Full Text Available In the crystal structure of the title compound, [Cd(C15H14NO22(C6H7NO2]n, the Cd atom displays a distorted octahedral geometry, including two pyridine N atoms and two hydroxyl O from four symmetry-related 3-pyridylmethanol (3-pyme ligands and two carboxylate O atoms from mefenamate [2-(2,3-dimethylanilinobenzoate] anions. The Cd atoms are connected via the bridging 3-pyme ligands into chains, that extend in the a-axis direction. The Cd atom is located on a center of inversion, whereas the 3-pyme ligands and the mefenamate anions occupy general positions.

  5. The gene for human U2 snRNP auxiliary factor small 35-kDa subunit (U2AF1) maps to the progressive myoclonus epilepsy (EPM1) critical region on chromosome 21q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Lalioti, M.D.; Rossier, C.; Antonarakis, S.E. [Univ. of Geneva Medical School (Switzerland)] [and others

    1996-04-15

    We used targeted exon trapping to clone portions of genes from human chromosome 21q22.3. One trapped sequence showed complete homology with the cDNA of human U2AF{sup 35} (M96982; HGM-approved nomenclature U2AF1), which encodes for the small 35-kDa subunit of the U2 snRNP auxiliary factor. Using the U2AF1 cDNA as a probe, we mapped this gene to cosmid Q15D2, a P1, and YAC 350F7 of the Chumakov et al. contig, close to the cystathionine-{beta}-synthase gene (CBS) on 21q22.3. This localization was confirmed by PCR using oligonucleotides from the 3{prime} UTR and by FISH. As U2AF1 associated with a number of different factors during mRNA splicing, overexpression in trisomy 21 individuals could contribute to some Down syndrome phenotypes by interfering with the splicing process. Furthermore, because this gene maps in the critical region for the progressive myoclonus epilepsy I locus (EPM1), mutation analysis will be carried out in patients to evaluate the potential role of U2AF1 as a candidate for EPM1. 24 refs., 1 fig.

  6. 伴有ins(8;21)(q22;q22.1q22.3)髓系白血病的荧光原位杂交研究%Fluorescence in situ hybridization studies on a myeloid leukemia patient with ins (8;21) (q22;q22.1q22.3)

    Institute of Scientific and Technical Information of China (English)

    吴亚芳; 马勤芬; 薛永权; 白淑潇; 张俊; 姚利; 王勇; 仇惠英; 沈娟; 潘金兰

    2009-01-01

    Objective To report a case of acute myeloid leukemia (AML) with the insertion (8;21) (q22; q22.1q22.3). A 33-year-old Chinese woman was referred to our hospital. Hematologic data showed WBC 42.7 ~ 109/L with monocytosis (monocyte counts 7.296 X 109/L). Bone marrow aspirate was hypercellular with 4.5% monoblasts and 7.5% promonocytes. At first she was diagnosed with chronic myelomonocytic leukemia (CMML) according to the FAB criteria. Initially the patient received supportive care only, but her general condition rapidly became worse three months later. The monoblasts and promonocytes in the bone marrow rose to 20.50%. After two cycles of combined chemotherapy she obtained complete remission. Methods Chromosome specimens were prepared by short-term culture of bone marrow cells. Karyotype analysis was carried out by R-banding technique. Three fluorescence in situ hybridization (FISH) analyses were performed using AML1-ETO dual color, dual fusion probe, whole chromosome painting 8 and 21 probes, and cen-8 and Tel 21qter probes, respectively. Reverse transcription polyrnerase chain reaction(RT-PCR) assay for detecting the AML1-ETO fusion transcript was also performed. Results Conventional cytogeuetie analysis showed a karyotype of 46,XX,ins(8;21) (q22;q22.1q22.3)[7]/46,XX[3]. FISH tests confirmed the insertion. RT-PCR analysis detected the AML1-ETO fusion transcript. Conclusion We consider that this patient should be rediagnosed as acute myeloid leukemia according to the criteria proposed by World Health Organization (WHO) and that FISH and RT-PCR play an important role in verification of the ins(8;21).%目的 对1例临床诊断为慢性粒单核细胞白血病患者进行荧光原位杂交(fluorescence in situhybridization,FISH)研究.方法 将患者骨髓细胞24 h培养后按常规方法制备染色体.采用R显带技术进行染色体核型分析;采用AML1-ETO双色双融合探针、8号和21号整条染色体涂染探针、8号染色体着丝粒探针和21号

  7. Correlations Between Leptin Gene Polymorphisms 223 A/G, 1019 G/A, 492 G/C, 976 C/A, and Anthropometrical and Biochemical Parameters in Children With Obesity: A Prospective Case-Control Study in a Romanian Population-The Nutrichild Study.

    Science.gov (United States)

    Mărginean, Cristina Oana; Mărginean, Claudiu; Voidăzan, Septimiu; Meliţ, Lorena; Crauciuc, Andrei; Duicu, Carmen; Bănescu, Claudia

    2016-03-01

    The aim of this study was to establish the manner in which the LEPR 223, 1019, 492, and 976 gene polymorphisms influence child obesity.We performed a prospective case-control study on 264 hospitalized children from Romania (Nutrichild study) whom we divided into 2 groups: Group I -143 controls and Group II-121 obese children.The 2 groups were evaluated regarding the anthropometry (MUAC, TST, H/L, hip, and abdominal circumference), paraclinical results (protein, leptin, adiponectin, TNF alfa, IL 6, IL 8, VEGF, protein, albumin) and LEPR 223, 1019, 492, and 976 gene polymorphisms. We noticed that the most frequent genotypes in obese children were AG+GG for LEPR 223 gene (P = 0.0001) and GA+AA for LEPR 1019 gene (P = 0.0001), whereas LEPR 492 and LEPR 976 gene polymorphisms did not correlate with obesity. MUAC, TST, H/L, leptin, and adiponectin were correlated with the GG genotype of the LEPR 223 gene, whereas the AG genotype correlated with TNF alpha and serum IL 8. Hip and abdominal perimeters were higher in LEPR 1019 AA genotype carriers, whereas TNF alpha and IL 6 correlated with the GG genotype of the same gene. Obesity did not correlate with protein serum levels.We observed that obesity is more frequent in children with LEPR 223 AG+GG and LEPR 1019 GA+AA genotypes. In obese children LEPR 223/492/1019 AG/GG/GA, GG/GG/GA and AA/GG/GA combined genotypes are more frequent.

  8. Dosimetric characterization of a 2-D array of 223 solid state detectors for daily morning checks in Tomo Therapy equipment; Caracterizacion dosimetrica de un arreglo 2D de 223 detectores de estado solido para verificaciones matutinas diarias en un equipo de Tomo Terapia

    Energy Technology Data Exchange (ETDEWEB)

    Reyes S, U.; Sosa A, M. [Universidad de Guanajuato, Division de Ciencias e Ingenieria, Lomas del Bosque No. 103, Col. Lomas del Campestre, 37150 Leon, Guanajuato (Mexico); Vega C, H. R., E-mail: uvaldoreyes@hotmail.com [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Cipres No. 10, Fracc. La Penuela, 98068 Zacatecas, Zac. (Mexico)

    2015-10-15

    Tomo Therapy is a new technique for the cancer treatment; however, the equipment must meet nearly all mechanical and dosimetric characteristics of a conventional linear accelerator for medical use. Daily quality controls are vital to the good operation of the equipment and thus guarantee excellent quality in the daily delivery of treatments. This paper presents the procedure of the dosimetric characterization of a two-dimensional array of 223 solid state detectors, called TomoDose of the Sun Nuclear Company. Dosimetric important criteria are established to perform these checks quickly and accurately. Dosimetric tests proposed are: repeatability, linearity, dependence of Sad and SSD. Some results are compared with readings of the ionization chamber Exradim A1SL. Finally the results of 30 consecutive days are presented to establish criteria for evidence of dose, field size, symmetry and flattening of the radiation beam on Tomo Therapy equipment. Expected values for daily verification are: Dose constancy of 194.89 c Gy, σ= 1.31 c Gy, symmetry in the X axis of -0.19 %, σ=0.08 %, symmetry in the Y axis of 1.66 %, σ= 0.05 %, flattened in the X axis of 25.71 %, σ= 0.05 % and flattened in the Y axis of 6.41 %, σ= 10.23 %. Field sizes obtained were 40.45 cm in the X axis and 5.10 on the Y axis, with standard deviations of 0.02 cm and 0.01 cm, respectively. TomoDose dosimetric values, compared to the values obtained with ionization chamber, presented differences smaller than 2%. (Author)

  9. MicroRNA-223-3p inhibits the angiogenesis of ischemic myocardial microvascular endothelial cells via modulating Rps6kb1/HIF-1α signal pathway%微小RNA-223-3p通过调节Rps6kb1/HIF-1α信号通路抑制缺血心肌微血管内皮细胞血管新生

    Institute of Scientific and Technical Information of China (English)

    戴国华; 宋宪波; 马培泽; 刘宁; 姚静

    2014-01-01

    Objective To explore the role of microRNA on the myocardial microvascular endothelial cells (CMECs) of ischemic heart rats in the process of angiogenesis and related regulation mechanism.Methods Myocardial ischemic rats model was established by coronary ligation.Seven days after operation,the ischemic CMECs were cultured by the method of planting myocardium tissue and identified by immunocytochemistry to observe the biological characteristics of ischemic CMECs angiogenesis,to determine the window period of migration,proliferation,tube formation in the process of its angiogenesis.Dynamic expression changes of microRNA in the process of ischemic CMECs angiogenesis was detected using microRNA chip and further verified by real-time PCR,the core microRNA of the ischemic CMECs was defined and the predicted target genes of core microRNA were determined by bioinformatics methods and real-time PCR.At the same time,the protein expression of target gene and angiogenesis related genes of p38MAPK,PI3K,Akt,VEGF were measured by Western blot.Results The CMECs of rats presented typical characteristics of microvascular endothelial cells,and factor Ⅷ,CD3 1 related antigens were all positively stained by immunocytochemical analysis.The migration window period was on the first day,and the tube formation window period was on the second day of both control and ischemic groups,while the proliferation window period was on the third day for the normal group,and the sixth day for ischemic group.According to the expressional difference and their relationship with angiogenesis,miRNA-223-3p was ultimately determined as the core microRNA in the process of ischemic CMECs angiogenesis,real-time PCR verified this hypothesis.Bioinformatics methods predicted that Rps6kb1 is the target genes of miRNA-223-3p,the pathway analysis showed that Rps6kb1 could regulate angiogenesis via HIF-1α signal pathway.Moreover,the mRNA and protein expression of VEGF,p38MAPK,PI3K,Akt,which were the downstream

  10. Variations in HDL-carried miR-223 and miR-135a concentrations after consumption of dietary trans fat are associated with changes in blood lipid and inflammatory markers in healthy men - an exploratory study.

    Science.gov (United States)

    Desgagné, Véronique; Guay, Simon-Pierre; Guérin, Renée; Corbin, François; Couture, Patrick; Lamarche, Benoit; Bouchard, Luigi

    2016-06-02

    A high consumption of trans fatty acids (TFAs) is associated with an increased risk of cardiovascular diseases (CVDs). High-density lipoproteins (HDLs) have many cardioprotective properties and transport functional microRNAs (miRNAs) to recipient cells. We hypothesized that dietary TFAs modify the HDL-carried miRNA profile, therefore modulating its cardioprotective properties. We assessed whether consumption of dietary TFAs modifies HDL-carried miR-223-3p and miR-135a-3p concentration and the inter-relationship between diet-induced changes in HDL-carried miRNA concentration and CVD risk markers. In a double blind, randomized, crossover, controlled study, 9 men were fed each of 3 experimental isoenergetic diets: 1) High in industrial TFA (iTFA; 3.7% energy); 2) High in TFA from ruminants (rTFA; 3.7% energy); 3) Low in TFA (control; 0.8% energy) for 4 weeks each. HDLs were isolated by ultracentrifugation and miRNAs were quantified by RT-qPCR. Variations in HDL-miR-223-3p concentration were negatively correlated with variations in HDL-cholesterol after the iTFA diet (rs = 0.82; P = 0.007), and positively correlated with variations in C-reactive protein concentration after the rTFA diet (rs = 0.75; P = 0.020). Variations in HDL-miR-135a-3p concentration were positively correlated with variations in total triglyceride (TG) concentration following the iTFA diet (rs = -0.82; P = 0.007), and with variations in low-density lipoprotein (LDL)-TG concentration following the rTFA diet (rs = 0.83; P = 0.005), compared to the control diet. However, the consumption of dietary TFAs has no significant unidirectional impact on HDL-carried miR-223-3p and miR-135a-3p concentrations. Our results suggest that the variability in the HDL-carried miRNAs response to TFA intake, by being associated with variations in CVD risk factors, might reflect physiological changes in HDL functions.

  11. Molecular cytogenetic characterization of an interstitial deletion of chromosome 21 (21q22.13q22.3) in a patient with dysmorphic features, intellectual disability and severe generalized epilepsy.

    Science.gov (United States)

    Valetto, Angelo; Orsini, Alessandro; Bertini, Veronica; Toschi, Benedetta; Bonuccelli, Alice; Simi, Francesca; Sammartino, Irene; Taddeucci, Grazia; Simi, Paolo; Saggese, Giuseppe

    2012-05-01

    We report on a de novo interstitial deletion of chromosome 21q in a patient presenting with characteristic facial features, intellectual disability, and epilepsy. The deletion extent was about 4.9 Mb from position 37713441 bp (21q22.13) to position 42665162 bp (21q22.3) (NCBI36/hg18 map). Patients with partial monosomy 21 are quite rare; this anomaly has been associated with a wide spectrum of clinical signs, ranging from very mild to quite severe phenotypes. This variability results from variability in the deleted regions, thus accurate molecular definition of the chromosomal breakpoints is necessary to make better genotype-phenotype correlations. We compared our patient's phenotype with the few other patients reported in the literature and found to have similar deletion when analyzed by array CGH. The minimal overlapping region contains only two genes, DYRK1A and KCNJ6, which may play a major role in these patients' phenotype.

  12. Genome-wide association analysis of genetic generalized epilepsies implicates susceptibility loci at 1q43, 2p16.1, 2q22.3 and 17q21.32

    DEFF Research Database (Denmark)

    Steffens, M.; Leu, C.; Ruppert, A. K.

    2012-01-01

    myoclonic epilepsy (JME). Joint Stage-1 and 2 analyses revealed genome-wide significant associations for GGEs at 2p16.1 (rs13026414, P-meta 2.5 10(9), OR[T] 0.81) and 17q21.32 (rs72823592, P-meta 9.3 10(9), OR[A] 0.77). The search for syndrome-related susceptibility alleles identified significant...... with the largest number of known epilepsy-related mutations. The associated regions harbor high-ranking candidate genes: CHRM3 at 1q43, VRK2 at 2p16.1, ZEB2 at 2q22.3, SCN1A at 2q24.3 and PNPO at 17q21.32. Further replication efforts are necessary to elucidate whether these positional candidate genes contribute...

  13. catena-Poly[[bis­[2-(2,3-dimethyl­anilino)benzoato-κO]cadmium(II)]-di-μ-3-pyridylmethanol-κ2 N:O;κ2 O:N

    Science.gov (United States)

    Moncol, Jan; Mikloš, Dušan; Segľa, Peter; Koman, Marian; Lis, Tadeusz

    2008-01-01

    In the crystal structure of the title compound, [Cd(C15H14NO2)2(C6H7NO)2]n, the Cd atom displays a distorted octa­hedral geometry, including two pyridine N atoms and two hydroxyl O from four symmetry-related 3-pyridylmethanol (3-pyme) ligands and two carboxylate O atoms from mefenamate [2-(2,3-dimethyl­anilino)benzoate] anions. The Cd atoms are connected via the bridging 3-pyme ligands into chains, that extend in the a-axis direction. The Cd atom is located on a center of inversion, whereas the 3-pyme ligands and the mefenamate anions occupy general positions. PMID:21201841

  14. A 46,X,der(X)t(X;Y)(p22.3;q11.2) karyotype found in a newborn with microcornea and sclerocornea: An example of the MLS/Goltz/Aicardi contiguous gene syndrome?

    Energy Technology Data Exchange (ETDEWEB)

    Milatovich, A.; Becker, T.; Kaufman, A. [Children`s Hospital Research Foundation, Cincinnati, OH (United States)] [and others

    1994-09-01

    There have been 15 reports in the literature of patients with microphthalmia and linear skin defects (MLS) who have deletions of the distal region of the short arm of the X chromosome, Xp22.3-pter. Since there appears to be some overlap of features between MLS, Goltz and Aicardi syndromes, it has been suggested that these syndromes represent a contiguous gene syndrome. At least three individuals have been reported with t(X;Y) and some common clinical features. We have seen a female infant with several anterior chamber anomalies including microcornea and sclerocornea, who also has an unbalanced translocation between the X and Y chromosomes, 46,X,der(X)t(X;Y) (p22.3;q11.2). Q-banding and fluorescence in situ hybridization (FISH) with a Y chromosome cocktail (Oncor) confirmed the identity of this derivative chromosome. X-inactivation studies done by RBG-banding showed that the der(X) is preferentially inactivated in all 50 metaphase cells examined from PHA-stimulated lymphocytes. It is likely some gene(s) on the distal Xp do not escape inactivation and when deleted result in anterior chamber abnormalities. X-inactivation patterns may be different in different tissues or at different times during development. Molecular studies will be helpful in determining what gene(s) are actually deleted in this case. This case is important because the clinical findings are limited to the development of the eyes and may help to define the critical regions and/or minimum region of overlap among MLS, Goltz and Aicardi syndromes.

  15. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228

    Energy Technology Data Exchange (ETDEWEB)

    Aleissa, Khalid A.; Almasoud, Fahad I.; Islam, Mohammed S. [Atomic Energy Research Institute, King Abdul Aziz City for Science and Technology, P.O. Box 6086, Riyadh 11442 (Saudi Arabia); L' Annunziata, Michael F. [IAEA Expert, Montague Group, P.O. Box 5033, Oceanside, CA 92052-5033 (United States)], E-mail: mlannunziata@cox.net

    2008-12-15

    The activities of {sup 228}Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide {sup 228}Ac. The radium was pre-concentrated on MnO{sub 2} and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter {sup 228}Ra({sup 228}Ac), the daughter nuclide {sup 228}Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by {sup 228}Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9{+-}0.1% was measured for {sup 228}Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317{+-}0.013 cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1 g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of {sup 228}Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for {sup 228}Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure {sup 228}Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  16. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228.

    Science.gov (United States)

    Aleissa, Khalid A; Almasoud, Fahad I; Islam, Mohammed S; L'Annunziata, Michael F

    2008-12-01

    The activities of (228)Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide (228)Ac. The radium was pre-concentrated on MnO(2) and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter (228)Ra((228)Ac), the daughter nuclide (228)Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by (228)Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9+/-0.1% was measured for (228)Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317+/-0.013cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of (228)Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for (228)Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure (228)Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  17. Purification of selenium from thorium, uranium, radium, actinium and potassium impurities for low background measurements

    Energy Technology Data Exchange (ETDEWEB)

    Rakhimov, A.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz); Warot, G. [CEA-CNRS, Modane (France). Laboratoire Souterrain de Modane (LSM); Karaivanov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Institute for Nuclear Research and Nuclear Energy (INRNE), Sofia (Bulgaria); Kochetov, O.I.; Lebedev, N.A.; Filosofov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Mukhamedshina, N.M.; Sadikov, I.I. [Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz)

    2013-07-01

    A technique of selenium purification from {sup 232}Th, {sup 238}U, {sup 226,228}Ra, {sup 227}Ac and {sup 40}K was developed. This technique is simple to perform and employs a minimum number of highly pure reagents (bidistilled water, nitric acid). Operations carried out during purification (elution, evaporation) practically exclude losses of the target product (chemical yields of Se > 99%). A test purification of 100 g of selenium was carried out using this technique. The efficiency of this technique was confirmed by low background gamma spectrometry of the purified selenium sample. Distribution coefficients of Th, U, Ra and Ac on DOWEX 50W- x 8 cation-exchange resin at different concentrations of selenium and nitric acid were experimentally determinated. Instrumental neutron activation analysis of bidistilled water, deionized water and nitric acid was performed. (orig.)

  18. Plasma levels of leptin and soluble leptin receptor and polymorphisms of leptin gene -18G > A and leptin receptor genes K109R and Q223R, in survivors of childhood acute lymphoblastic leukemia

    Directory of Open Access Journals (Sweden)

    Gozdzik Jolanta

    2011-06-01

    Full Text Available Abstract Background Approximately 20% of children and adolescents in Europe are overweight. Survivors of pediatric acute lymphoblastic leukemia (ALL are at increased risk of overweight and obesity. The purpose of this study was to assess leptin and leptin soluble receptor levels, as well as polymorphisms of selected genes in survivors of pediatric ALL, and the influence of chemo- and radiotherapy on development of overweight in the context of leptin regulation. Methods Eighty two patients (55% males, of median age 13.2 years (m: 4.8 years; M: 26.2 years were included in the study. The ALL therapy was conducted according to modified Berlin-Frankfurt-Munster (BFM; n = 69 regimen or New York (n = 13 regimen. In 38% of patients cranial radiotherapy (CRT was used in median dose of 18.2Gy (m: 14Gy; M: 24Gy. Median age at diagnosis was 4.5 (m: 1 year; M: 16.9 years and median time from completion of ALL treatment was 3.2 years (m: 0.5 year; M: 4.3 years. Patients with BMI ≥85 percentile were classified as overweight. Correlation of plasma levels of leptin and leptin soluble receptor, and polymorphisms of leptin gene -18G > A, leptin receptor genes K109R and Q223R, and the overweight status were analyzed in relation to gender, intensity of chemotherapy (high intensity vs. standard intensity regimens and to the use of CRT. Results Significant differences of leptin levels in patients treated with and without CRT, both in the entire study group (22.2+/- 3.13 ng/ml vs. 14.9+/-1.6 ng/ml; p Conclusions The prevalence of overweight in our cohort was higher than in general European population (31% vs 20% and increased regardless of the use of CRT. Leptin and leptin receptor levels may be used as useful markers of high risk of becoming overweight in ALL survivors, particularly in females treated with CRT. Polymorphisms of leptin gene -18G > A and leptin receptor genes K109R and Q223R were not associated with overweight status in ALL survivors.

  19. Clinical analysis on the loop electrosurgical excision procedure in the treatment of 131 patients with cervical lesions%环形电切术治疗宫颈病变223例临床分析

    Institute of Scientific and Technical Information of China (English)

    常业莎; 吕燕玲; 容俊; 朱瑜

    2012-01-01

    Objective To explore the clinical value of loop electrosurgical excision procedure (LEEP) in the diagnosis and treatment of cervical lesions. Methods The clinical datas of 223 cases with cervical lesions in our hospital from January 2010 to December 2010 treated with loop electrosurgical excision procedure were analyzed. Results The average operating time was (5.38±1.38) min. The average amount of bleeding in surgery was (6.02±3.24) mL. 17 patients treated with LEEP bleed more than 40 mL after the operation. The cases which pathological findings before the operation accorded with patho -logical results after loop electrosurgical excision procedure were 216 cases. The case degrading after the operation was 1 case; and which escalating was 6 cases. Conclusion Loop electrosurgical excision procedure has significant value in the treatment of cervical lesions, because of shorter operating time, lesser bleeding in operation and providing intact pathologi -ca ses.%目的 探讨环形电切术(LEEP)在宫颈病变的诊断及治疗中的临床价值.方法 回顾性分析2010年1~12月我院用高频电波刀行环行电切除术的223例宫颈病变患者的临床资料.结果 时间平均手术(5.38±1.38)min,平均术中出血量(6.02±3.24)mL,术后阴道流血量>40 mL 17例,术前阴道镜下点活检病理诊断与环形电切术后病理诊断完全符合216例,降级1例,升级6例.结论 环形电切术治疗宫颈病变有手术时间短、术中出血少、可提供完整的病理标本等优势,值得在临床广泛使用.

  20. Array CGH characterization of an unbalanced X-autosome translocation associated with Xq27.2-qter deletion, 11q24.3-qter duplication and Xq22.3-q27.1 duplication in a girl with primary amenorrhea and mental retardation.

    Science.gov (United States)

    Chen, Chih-Ping; Lin, Shuan-Pei; Chern, Schu-Rern; Kuo, Yu-Ling; Wu, Peih-Shan; Chen, Yu-Ting; Lee, Meng-Shan; Wang, Wayseen

    2014-02-01

    We present array comparative genomic hybridization (aCGH) characterization of an unbalanced X-autosome translocation with an Xq interstitial segmental duplication in a 16-year-old girl with primary ovarian failure, mental retardation, attention deficit disorder, learning difficulty and facial dysmorphism. aCGH analysis revealed an Xq27.2-q28 deletion, an 11q24.3-q25 duplication, and an inverted duplication of Xq22.3-q27.1. The karyotype was 46,X,der(X)t(X;11)(q27.2;q24.3) dup(X)(q27.1q22.3). We discuss the genotype-phenotype correlation in this case. Our case provides evidence for an association of primary amenorrhea and mental retardation with concomitant unbalanced X-autosome translocation and X chromosome rearrangement.

  1. Synthesis, Crystal Structure and Biological Activities of 1-((5-(4-(4-Chlorophenoxy)-2-chlorophenyl)-2,2,3-trimethyloxazolidin-5-yl)methyl)-1H-1,2,4-triazole

    Institute of Scientific and Technical Information of China (English)

    XU Liang-Zhong; BI Wen-Zhao; YANG Zhi; MENG Fan-Lei; ZHU Qi

    2008-01-01

    The title compound 1-((5-(4-(4-chlorophenoxy)-2-chlorophenyl)-2,2,3-trimethyl-oxazolidin-5-yl)methyl)-1H-1,2,4-triazole (C21H22Cl2N4O2) has been synthesized and characterized by elemental analysis, IR, 1H NMR, MS and single-crystal X-ray diffraction. The crystal belongs to the triclinic system, space group P1 with a = 6.891(2), b = 9.074(2), c = 18.258(4)(A), α = 99.292(4), β = 95.105(4), γ = 108.068(3)°, C21H22Cl2N4O2, Mr = 433.33, V = 1059.3(4) (A)3, Z = 2, Dc = 1.359 g/cm3, F(000) = 452, μ = 0.331 mm-1, the final R = 0.0448 and wR = 0.0994 for 3727 unique reflections. The dihedral angle between the oxazolidine ring taking an envelope conformation with a local pseudo-mirror and the triazole ring is 27.7(9)°. Weak intermolecular C-H…N hydrogen bonds and π-π interactions exist between the triazole rings of neighboring molecules, forming a three-dimensional network, which stabilizes the crystal structure. The primary biological test shows the target compound has certain fungicidal activity.

  2. [O III] emission line as a tracer of star-forming galaxies at high redshifts: comparison between Hα and [O III] emitters at z=2.23 in HiZELS

    Science.gov (United States)

    Suzuki, T. L.; Kodama, T.; Sobral, D.; Khostovan, A. A.; Hayashi, M.; Shimakawa, R.; Koyama, Y.; Tadaki, K.-i.; Tanaka, I.; Minowa, Y.; Yamamoto, M.; Smail, I.; Best, P. N.

    2016-10-01

    We investigate the properties of z = 2.23 Hα and [O III] λ5007 emitters using the narrow-band-selected samples obtained from the High-z Emission Line Survey. We construct two samples of the Hα and [O III] emitters and compare their integrated physical properties. We find that the distribution of stellar masses, dust extinction, star formation rates (SFRs), and specific SFRs (sSFRs) is not statistically different between the two samples. When we separate the full galaxy sample into three subsamples according to the detections of the Hα and/or [O III] emission lines, most of the sources detected with both Hα and [O III] show log(sSFRUV) ≳ -9.5. The comparison of the three subsamples suggests that sources with strong [O III] line emission tend to have the highest star-forming activity out all galaxies that we study. We argue that the [O III] emission line can be used as a tracer of star-forming galaxies at high redshift, and that it is especially useful to investigate star-forming galaxies at z > 3, for which Hα emission is no longer observable from the ground.

  3. [OIII] emission line as a tracer of star-forming galaxies at high redshifts: Comparison between H$\\alpha$ and [OIII] emitters at $z$=2.23 in HiZELS

    CERN Document Server

    Suzuki, T L; Sobral, D; Khostovan, A A; Hayashi, M; Shimakawa, R; Koyama, Y; Tadaki, K -i; Tanaka, I; Minowa, Y; Yamamoto, M; Smail, I; Best, P N

    2016-01-01

    We investigate the properties of $z$=2.23 H$\\alpha$ and [OIII]$\\lambda$5007 emitters using the narrow-band-selected samples obtained from the High-$z$ Emission Line Survey (HiZELS: Sobral et al. 2013). We construct two samples of the H$\\alpha$ and [OIII] emitters and compare their integrated physical properties. We find that the distribution of stellar masses, dust extinction, star formation rates (SFRs), and specific SFRs, is not statistically different between the two samples. When we separate the full galaxy sample into three subsamples according to the detections of the H$\\alpha$ and/or [OIII] emission lines, most of the sources detected with both H$\\alpha$ and [OIII] show ${\\rm log(sSFR_{UV})}$$\\gtrsim$-9.5. The comparison of the three subsamples suggests that sources with strong [OIII] line emission tend to have the highest star-forming activity out all galaxies that we study. We argue that the [OIII] emission line can be used as a tracer of star-forming galaxies at high redshift, and that it is especia...

  4. Multiple tumor types including leiomyoma and Wilms tumor in a patient with Gorlin syndrome due to 9q22.3 microdeletion encompassing the PTCH1 and FANC-C loci.

    Science.gov (United States)

    Garavelli, Livia; Piemontese, Maria Rosaria; Cavazza, Alberto; Rosato, Simonetta; Wischmeijer, Anita; Gelmini, Chiara; Albertini, Enrico; Albertini, Giuseppe; Forzano, Francesca; Franchi, Fabrizia; Carella, Massimo; Zelante, Leopoldo; Superti-Furga, Andrea

    2013-11-01

    Gorlin syndrome or nevoid basal cell carcinoma syndrome (NBCCS) is an autosomal dominant condition mainly characterized by the development of mandibular keratocysts which often have their onset during the second decade of life and/or multiple basal cell carcinoma (BCC) normally arising during the third decade. Cardiac and ovarian fibromas can be found. Patients with NBCCS develop the childhood brain malignancy medulloblastoma (now often called primitive neuro-ectodermal tumor [PNET]) in 5% of cases. The risk of other malignant neoplasms is not clearly increased, although lymphoma and meningioma can occur in this condition. Wilms tumor has been mentioned in the literature four times. We describe a patient with a 10.9 Mb 9q22.3 deletion spanning 9q22.2 through 9q31.1 that includes the entire codifying sequence of the gene PTCH1, with Wilms tumor, multiple neoplasms (lung, liver, mesenteric, gastric and renal leiomyomas, lung typical carcinoid tumor, adenomatoid tumor of the pleura) and a severe clinical presentation. We propose including leiomyomas among minor criteria of the NBCCS.

  5. Analysis of 133 meioses places the genes for nevoid basal cell carcinoma (gorlin) syndrome and fanconi anemia group C in a 2.6-cM interval and contributes to the fine map of 9q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Farndon, P.A.; Hardy, C.; Kilpatrick, M.W. [Birmingham Maternity Hospital, Edgbaston (United Kingdom)] [and others

    1994-09-15

    Four disease genes (NBCCS, ESS1, XPAC, FACC) map to 9q22.3-q31. A fine map of this region was produced by linkage and haplotype analysis using 12 DNA markers. The gene for nevoid basal cell carcinoma syndrome (NBCCS, Gorlin) has an important role in congenital malformations and carcinogenesis. Phase-known recombinants in a study of 133 meioses place NBCCS between (D9S12/D9S151) and D9S176. Haplotype analysis in a two-generation family suggests that NBCCS lies in a smaller interval of 2.6 cM centromeric to D9S287. These flanking markers will be useful clinically for gene tracking. Recombinants also map FACC (Fanconi anemia, group C) to the same region, between (D9S12/D9S151) and D9S287. The recombination rate between (D9S12/D9S151) and D9S53 in males is 8.3% and 13.2% in females, giving a sex-specific male:female ratio of 1:1.6 and a sex-averaged map distance of 10.4 cM. No double recombinants were detected, in agreement with the apparently complete level of interference predicted from the male chiasmata map. 19 refs., 2 figs., 1 tab.

  6. Human fructose-1,6-bisphosphatase gene (FBP1): Exon-intron organization, localization to chromosome bands 9q22.2-q22.3, and mutation screening in subjects with fructose-1,6-bisphosphatase deficiency

    Energy Technology Data Exchange (ETDEWEB)

    El-Maghrabi, M.R.; Jiang, W. [State Univ. of New York, Stony Brook, NY (United States)] [and others

    1995-06-10

    Fructose-1,6-bisphosphatase (EC 3.1.3.11) is a key regulatory enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose-1,6-bisphosphate to generate fructose-6-phosphate and inorganic phosphate. Deficiency of fructose-1,6-bisphosphatase is associated with fasting hypoglycemia and metabolic acidosis because of impaired gluconeogenesis. We have cloned and characterized the human liver fructose-1,6-bisphosphatase gene (FBP1). FBP1, localized to chromosome bands 9q22.2-q22.3 by fluorescence in situ hybridization, consists of seven exons that span > 31 kb, and the six introns are in the same position as in the rat gene. FBP1 was screened for mutations in two subjects with fructose-1,6-bisphosphatase deficiency. Four nucleotide substitutions were identified, two of which were silent mutations in the codons for Ala-216 (GCT {yields} GCC) and Gly-319 (GGG {yields} GGA). The other substitutions were in intron 3, a C {yields} T substitution 7 nucleotides downstream from the splice donor site, and in the promoter region, an A {yields} T substitution 188 nucleotides upstream from the start of transcription. These nucleotide substitutions were also found in normal unaffected subjects and thus are not the cause of fructose-1,6-bisphosphatase deficiency in the two subjects studied. The molecular basis of hepatic fructose-1,6-bisphosphatase deficiency in these subjects remains undetermined but could result from unidentified mutations in the promoter that decrease expression or from mutations in another gene that indirectly lead to decreased fructose-1,6-bisphosphatase activity. 18 refs., 3 figs., 3 tabs.

  7. Grievance Arbitration in Education. Fastback 223.

    Science.gov (United States)

    Lovell, Ned B.

    This pamphlet provides information necessary to analyze existing grievance procedures and sets forth principles to guide the formulation of a more effective grievance process. The introduction defines the grievance procedure in a contractual contest, describes its benefits to management, union members, and private citizens; and briefly reviews key…

  8. 40 CFR 61.223 - Compliance procedures.

    Science.gov (United States)

    2010-07-01

    ... facility shall provide EPA with a report detailing the actions taken and the results of the radon-222 flux...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standards for Radon Emissions... completion of covering the pile to limit radon emissions but prior to the long term stabilization of the...

  9. 50 CFR 223.203 - Anadromous fish.

    Science.gov (United States)

    2010-10-01

    ... contractors of the ODFW, WDFW (Agencies), IDFG, or CDFG (Agencies), or as a part of a monitoring and research... Screening Criteria for Anadromous Salmonids, January 1997” or with any subsequent revision. (ii) The owner... require a commitment of compensatory mitigation if implementation of the plan and schedule is...

  10. 40 CFR 52.223 - Approval status.

    Science.gov (United States)

    2010-07-01

    ... the adoption and submittal of reasonably available control technology (RACT) requirements by July 1... January 1979 and adoption and submittal by each subsequent January of additional RACT requirements for... adoption of a new regional plan and ordinances. However, EPA disapproves the plan for any future...

  11. 22 CFR 223.11 - Appropriate action.

    Science.gov (United States)

    2010-04-01

    ... influence, any oral or written communication to, the Agency on any matter of business for a period not to... such appearance or to accept any such communication; and (b) Taking other appropriate...

  12. 40 CFR 745.223 - Definitions.

    Science.gov (United States)

    2010-07-01

    ...-care centers, preschools and kindergarten classrooms. Clearance levels are values that indicate the... establishment and operation of management and resident education programs. Lead-based paint means paint or other... components with large surface areas; or more than 2 square feet of deteriorated paint on interior...

  13. CopperCore 2.2.3

    NARCIS (Netherlands)

    Vogten, Hubert; Martens, Harrie; Koper, Rob

    2005-01-01

    Changes in this version: great number of bug fixes with regard to notifcation handling, allowed empty items in order to be more compatible with Reload, added QTI content type for CopperCore Service Integration, improved error handling, improved Clicc and fixed a bug regarding the else operation in a

  14. 36 CFR 223.231 - Bidding methods.

    Science.gov (United States)

    2010-07-01

    ...) Ensure open and fair competition; (b) Ensure that the Federal Government receives minimum rates or... prospectus. The Forest Service shall not accept a bid at oral auction that is less than the bidder's initial sealed bid; and (e) Specify the use of sealed bids or a mix of bidding methods in the affected area...

  15. 48 CFR 223.370-1 - Scope.

    Science.gov (United States)

    2010-10-01

    ... explosive end item or weapon system. (b) This section does not apply to acquisitions solely for— (1) Inert...) Oxidizers; (5) Powdered metals; or (6) Other materials having fire or explosive characteristics....

  16. 36 CFR 223.160 - Definitions.

    Science.gov (United States)

    2010-07-01

    ..., or the minimum piece specification set forth in the timber sale contract, in material meeting the... of size, sawn on four sides; (2) Chips, pulp, and pulp products; (3) Green veneer and plywood;...

  17. 36 CFR 223.186 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... country; (2) When unprocessed timber is placed in an export facility in preparation (sorting, bundling... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER The Forest Resources Conservation and Shortage Relief Act of 1990... exporting unprocessed Federal timber (including causing unprocessed timber to be exported). Willfully...

  18. 48 CFR 223.7201 - Policy.

    Science.gov (United States)

    2010-10-01

    ... DEFENSE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES... within the meaning of FAR 2.101; or (2) The contract will be performed in a Government-owned...

  19. 48 CFR 223.7102 - Exceptions.

    Science.gov (United States)

    2010-10-01

    ... OF DEFENSE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY... feasible means of disposal is not available to the contractor, taking into consideration public safety... Secretary of Energy; (6) The storage of materials that constitute military resources intended to be...

  20. 49 CFR 223.7 - Responsibility.

    Science.gov (United States)

    2010-10-01

    ... persons, or has caused death or injury, a penalty not to exceed $100,000 per violation may be assessed... requirement is subject to a civil penalty of at least $650 and not more than $25,000 per violation, except that: Penalties may be assessed against individuals only for willful violations, and, where a...

  1. 50 CFR 223.207 - Approved TEDs.

    Science.gov (United States)

    2010-10-01

    ... than 4 inches (10.2 cm) from the frame and may not extend past the frame. A horizontal brace bar to... the TED frame must not exceed 4 inches (10.2 cm). (5) Direction of bars. The deflector bars must run... bottom bars and two of the top bars, including the frame, may run from side to side of the TED....

  2. 32 CFR 223.3 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... for military applications at a fixed facility or in transit. (b) Authorized Individual. A person who... characteristics. (e) Information. Any fact or concept regardless of the physical form or characteristics of...

  3. Purification of radium-226 for the manufacturing of actinium-225 in a cyclotron for alpha-immunotherapy; Radium-Aufreinigung zur Herstellung von Actinium-225 am Zyklotron fuer die Alpha-Immuntherapie

    Energy Technology Data Exchange (ETDEWEB)

    Marx, Sebastian Markus

    2014-09-23

    The thesis describes the development of methods for the purification of Ra-226. The objective was to obtain the radionuclide in the quality that is needed to be used as starting material in the manufacturing process for Ac-225 via proton-irradiated Ra-226. The radionuclide has been gained efficiently out of huge excesses of impurities. The high purity of the obtained radium affords its use as staring material in a pharmaceutical manufacturing process.

  4. Renal uptake of bismuth-213 and its contribution to kidney radiation dose following administration of actinium-225-labeled antibody

    Energy Technology Data Exchange (ETDEWEB)

    Schwartz, J; O' Donoghue, J A; Humm, J L [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); Jaggi, J S [Bristol-Myers Squibb, Plainsboro, NJ (United States); Ruan, S; Larson, S M [Nuclear Medicine Service Department of Radiology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); McDevitt, M; Scheinberg, D A, E-mail: schwarj1@mskcc.org [Molecular Pharmacology and Chemistry, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10065 (United States)

    2011-02-07

    Clinical therapeutic studies using {sup 225}Ac-labeled antibodies have begun. Of major concern is renal toxicity that may result from the three alpha-emitting progeny generated following the decay of {sup 225}Ac. The purpose of this study was to determine the amount of {sup 225}Ac and non-equilibrium progeny in the mouse kidney after the injection of {sup 225}Ac-huM195 antibody and examine the dosimetric consequences. Groups of mice were sacrificed at 24, 96 and 144 h after injection with {sup 225}Ac-huM195 antibody and kidneys excised. One kidney was used for gamma ray spectroscopic measurements by a high-purity germanium (HPGe) detector. The second kidney was used to generate frozen tissue sections which were examined by digital autoradiography (DAR). Two measurements were performed on each kidney specimen: (1) immediately post-resection and (2) after sufficient time for any non-equilibrium excess {sup 213}Bi to decay completely. Comparison of these measurements enabled estimation of the amount of excess {sup 213}Bi reaching the kidney ({gamma}-ray spectroscopy) and its sub-regional distribution (DAR). The average absorbed dose to whole kidney, determined by spectroscopy, was 0.77 (SD 0.21) Gy kBq{sup -1}, of which 0.46 (SD 0.16) Gy kBq{sup -1} (i.e. 60%) was due to non-equilibrium excess {sup 213}Bi. The relative contributions to renal cortex and medulla were determined by DAR. The estimated dose to the cortex from non-equilibrium excess {sup 213}Bi (0.31 (SD 0.11) Gy kBq{sup -1}) represented {approx}46% of the total. For the medulla the dose contribution from excess {sup 213}Bi (0.81 (SD 0.28) Gy kBq{sup -1}) was {approx}80% of the total. Based on these estimates, for human patients we project a kidney-absorbed dose of 0.28 Gy MBq{sup -1} following administration of {sup 225}Ac-huM195 with non-equilibrium excess {sup 213}Bi responsible for approximately 60% of the total. Methods to reduce renal accumulation of radioactive progeny appear to be necessary for the success of {sup 225}Ac radioimmunotherapy.

  5. 1935 15' Quad #223 Aerial Photo Mosaic Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

  6. Failure modes and effects analysis (RADL Item 2-23)

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-04-01

    The Pilot Plant is a central receiver design concept. It is comprised of five major subsystems as shown schematically, plus a set of equipment (Plant Support Subsystem) used to support total plant operation. The failure modes and effects analysis (FMEA) is a bottom-up analysis used to identify the failure characteristics of the system (total equipment used to produce electrical power), that is, the failure of a single component is assumed and the effect of that failure upon the system is determined. The FMEA is concerned with the plant from an operational standpoint (i.e., the production of electrical power). This analysis was performed to the component level. This was interpreted as a valve, computer, measurement sensor and its associated signal conditioning, an electronic black box, etc.

  7. 19 CFR 10.223 - Articles eligible for preferential treatment.

    Science.gov (United States)

    2010-04-01

    ... States, and knitted or crocheted apparel articles (other than non-underwear t-shirts classifiable under...) Non-underwear t-shirts, classifiable under subheadings 6109.10.00 and 6109.90.10 of the HTSUS, made in... 1, 2002, and that contains a knitted or crocheted or woven fabric, or a knitted or crocheted...

  8. Dicty_cDB: VSI223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available EIC ACID AND POLYPEPTIDE SEQUENCES FROM LAWSO... 54 0.008 1 ( DD307082 ) NUCLEIC... ACID AND POLYPEPTIDE SEQUENCES FROM LAWSO... 54 0.008 1 ( DD300861 ) NUCLEIC ACID A..., clone RP23-74B10, comp... 56 0.002 1 ( AC140360 ) Mus musculus BAC clone RP24-241G4 from chromosome... 54 0.008 1 ( DD307412 ) NUCL

  9. 48 CFR 52.223-4 - Recovered Material Certification.

    Science.gov (United States)

    2010-10-01

    ... recovered materials content for EPA-designated items to be delivered or used in the performance of the contract will be at least the amount required by the applicable contract specifications or other... (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses...

  10. 36 CFR 223.221 - Establishing minimum rates.

    Science.gov (United States)

    2010-07-01

    ... sale of special forest products or groups of special forest products. Products must be sold for appraised value or minimum rates, whichever is higher. No products may be sold or harvested for less than... SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Appraisal and Pricing §...

  11. 36 CFR 223.187 - Determinations of unprocessed timber.

    Science.gov (United States)

    2010-07-01

    ... AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER The Forest Resources Conservation and Shortage... instance, because the timber originates from private land from which timber may be exported. (2) Lumber... and substitution restrictions of the Forest Resources Conservation and Shortage Relief Act of 1990...

  12. 36 CFR 223.185 - Scope and applicability.

    Science.gov (United States)

    2010-07-01

    ... AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER The Forest Resources Conservation and Shortage Relief... Forest Resources Conservation and Shortage Relief Act of 1990 (16 U.S.C. 620, et seq.) that became... to unprocessed timber originating from private lands west of the 100th meridian in the contiguous...

  13. Dicty_cDB: VFL223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ll=Heat shock 70 kDa protein; &A53163(A5316... 330 2e-89 EU679414_1( EU679414 |pid:none) Tetranychus cinna...barinus heat shoc... 330 2e-89 AY147871_1( AY147871 |pid:none) Rhizopus stolonifer

  14. 50 CFR 300.223 - Purse seine fishing restrictions.

    Science.gov (United States)

    2010-10-01

    ... seine fishing restrictions. All dates used in this section are in Universal Coordinated Time, also known... within the Convention Area takes place entirely within areas under U.S. jurisdiction or entirely within areas under jurisdiction of a single nation other than the United States. (ii) No fishing takes...

  15. 48 CFR 952.223-78 - Sustainable acquisition program.

    Science.gov (United States)

    2010-10-01

    ... constraints of the general design specifications. Compliance with the Guiding Principles for Federal... or environmentally sustainable products or services in the materials selection process. The... Government or Industry Internet Sites: (1) Recycled Content Products are described at http://epa.gov/cpg....

  16. 44 CFR 206.223 - General work eligibility.

    Science.gov (United States)

    2010-10-01

    ... eligible applicant. (b) Private nonprofit facilities. To be eligible, all private nonprofit facilities must be owned and operated by an organization meeting the definition of a private nonprofit organization... by an eligible applicant, as defined in § 206.222, must be owned by a private nonprofit...

  17. Dicty_cDB: CFH223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 0.009 12 AZ930073 |AZ930073.1 479.dil58d12.s1 Saccharomyces kluyveri Saccharomyce...s kluyveri genomic clone 479.dil58d12.s1, DNA sequence. 40 0.009 2 dna update 2004. 1.31 Homology vs Protein

  18. 17 CFR 256.223 - Advances from associate companies.

    Science.gov (United States)

    2010-04-01

    ... settlement which are includable in account 233, Notes payable to associate companies or account 234, Accounts... (CONTINUED) UNIFORM SYSTEM OF ACCOUNTS FOR MUTUAL SERVICE COMPANIES AND SUBSIDIARY SERVICE COMPANIES, PUBLIC... account shall include the principal amount of notes and balances on open accounts due to...

  19. 36 CFR 223.53 - Urgent removal contract extensions.

    Science.gov (United States)

    2010-07-01

    ... interest in extending National Forest System timber sale contracts for undamaged (green) timber not... granted when a specific catastrophic event beyond the control of the landowner occurs on non-National.... Catastrophic events include, but are not limited to, severe wildfire, wind, floods, insects and...

  20. 36 CFR 223.137 - Causes for debarment.

    Science.gov (United States)

    2010-07-01

    ... exportation of timber; (5) Observance of restrictions on the disposal of timber from small business set-aside... Service timber sale contracts so serious as to justify debarment, such as: (1) Willful failure to perform in accordance with contract; or (2) A history of failure to perform contract terms; or...

  1. Dicty_cDB: VSF223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 196315 |pid:none) Auxenochlorella protothecoides rib... 271 2e-71 BC054263_1( BC054263 |pid:none) Xenopus la...F402813 |pid:none) Ictalurus punctatus 40S ribosomal ... 271 2e-71 FJ196315_1( FJ

  2. Dicty_cDB: SHC223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rh...ynchosciara americana cDNA, mRNA sequence. 34 4e-05 3 CK808739 |CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning...e-04 1 CK808037 |CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara

  3. 36 CFR 223.275 - Establishment of a pilot program.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Establishment of a pilot... Establishment of a pilot program. This subpart governs the Forest Service's pilot program for the disposal of... of Title III of H.R. 3423)), as amended in 2004 by Section 335 of Public Law 108-108. The...

  4. 48 CFR 1552.223-72 - Care of laboratory animals.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Care of laboratory animals... Care of laboratory animals. As prescribed in 1523.303-72, insert the following clause: Care of... adhere to the principles enunciated in the Guide for Care and Use of Laboratory Animals prepared by...

  5. Dicty_cDB: SFH223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available r**iin*ikn*kkiiik*scyrc*sidtifskwtlcvygwicryrl skgstncigrpcgkkwitg*ikneficwsiswtrdrrslgnvgydr*eisspkwtshs*w yk*r*nsfcrstfidick...kwtlcvygwicryrl skgstncigrpcgkkwitg*ikneficwsiswtrdrrslgnvgydr*eisspkwtshs*w yk*r*nsfcrstfidick*FIGWILYIDKPH

  6. Dicty_cDB: VSJ223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available etical protein ZK381.7 - Caenorhabditis elegans. 46 0.43 1 AF277887 |AF277887.1 Sida crystallina clone 67SE ...5 unordered pieces. 44 1.7 1 AF277884 |AF277884.1 Sida crystallina clone 63NA cytochrome c oxidase subunit I... (COI) gene, partial cds; mitochondrial gene for mitochondrial product. 34 3.8 2 AF277883 |AF277883.1 Sida... 2 AF277888 |AF277888.1 Sida crystallina clone 68SE cytochrome c oxidase subunit ...cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial gene for mitochondrial product. 36 1.0

  7. 36 CFR 223.101 - Determination of purchaser responsibility.

    Science.gov (United States)

    2010-07-01

    ... purchaser has a satisfactory record of integrity and business ethics; (5) The purchaser has or is able to... and governmental business commitments; (3) The purchaser has a satisfactory performance record on... applicable laws and regulations. (c) If the prospective purchaser is a small business concern and...

  8. Dicty_cDB: SHE223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 5e-05 U59295_1( U59295 |pid:none) Placopecten magellanicus myosin heavy ... 51 7e...-05 U59294_1( U59294 |pid:none) Placopecten magellanicus myosin heavy ... 51 7e-05 AY823086_1( AY823086 |pid

  9. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin.

    Science.gov (United States)

    Nour, S; El-Sharkawy, A; Burnett, W C; Horwitz, E P

    2004-12-01

    The objective of this work was to establish a new procedure for 228Ra determination of natural waters via preconcentration of radium on MnO2 and separation of its daughter, 228Ac, using Diphonix ion exchange resin. Following removal of potential interferences via passage through an initial Diphonix Resin column, the first daughter of 228Ra, 228Ac, is isolated by chromatographic separation via a second Diphonix column. A holding time of > 30 h for 228Ac ingrowth in between the two column separations ensures secular equilibrium. Barium-133 is used as a yield tracer. Actinium-228 is eluted from the second Diphonix Resin with 5 ml 1M 1-Hydroxyethane-1,1-diphosphonic acid (HEDPA) and quantified by addition of scintillation cocktail and LSC counting. Radium (and 133Ba) from the load and rinse solutions from the 2nd Diphonix column may be prepared for alpha spectrometry (for determination of 223Ra, 224Ra, and 226Ra) by BaSO4 microprecipitation and filtration. Decontamination tests indicate that U, Th, and Ra series nuclides do not interfere with these measurements, although high contents of 90Sr (90Y) require additional treatment for accurate measurement of 228Ra. Addition of stable Sr as a "hold back" carrier during the initial MnO2 preconcentration step was shown to remove most 90Sr interference.

  10. Topographic Map of Quadrangle 3670, Jam-Kashem (223) and Zebak (224) Quadrangles, Afghanistan

    Science.gov (United States)

    Bohannon, Robert G.

    2006-01-01

    This map was produced from several larger digital datasets. Topography was derived from Shuttle Radar Topography Mission (SRTM) 85-meter digital data. Gaps in the original dataset were filled with data digitized from contours on 1:200,000-scale Soviet General Staff Sheets (1978-1997). Contours were generated by cubic convolution averaged over four pixels using TNTmips surface-modeling capabilities. Minor artifacts resulting from the auto-contouring technique are present. Streams were auto-generated from the SRTM data in TNTmips as flow paths. Flow paths were limited in number by their Horton value on a quadrangle-by-quadrangle basis. Peak elevations were averaged over an area measuring 85 m by 85 m (represented by one pixel), and they are slightly lower than the highest corresponding point on the ground. Cultural data were extracted from files downloaded from the Afghanistan Information Management Service (AIMS) Web site (http://www.aims.org.af). The AIMS files were originally derived from maps produced by the Afghanistan Geodesy and Cartography Head Office (AGCHO). Because cultural features were not derived from the SRTM base, they do not match it precisely. Province boundaries are not exactly located. This map is part of a series that includes a geologic map, a topographic map, a Landsat natural-color-image map, and a Landsat false-color-image map for the USGS/AGS (Afghan Geological Survey) quadrangles covering Afghanistan. The maps for any given quadrangle have the same open-file number but a different letter suffix, namely, -A, -B, -C, and -D for the geologic, topographic, Landsat natural-color, and Landsat false-color maps, respectively. The open-file report (OFR) numbers for each quadrangle range in sequence from 1092 - 1123. The present map series is to be followed by a second series, in which the geology is reinterpreted on the basis of analysis of remote-sensing data, limited fieldwork, and library research. The second series is to be produced by the USGS in cooperation with the AGS and AGCHO.

  11. Influencia del polimorfismo Gln223Arg del receptor de leptina en el sobrepeso

    OpenAIRE

    2011-01-01

    En la mayoría de las situaciones el sobrepeso/obesidad tiene un carácter poligénico, con múltiples genes implicados, con interacciones entre ellos y con el ambiente, sin que hasta la fecha se haya podido cuantificar la contribución real de cada uno de esos factores. Existen, sin embargo, grupos de genes que parecen ser determinantes en relación a la predisposición a los estados de sobrepeso. Entre los múltiples genes determinantes en la obesidad destacan aquellos implicados ...

  12. 50 CFR 223.102 - Enumeration of threatened marine and anadromous species.

    Science.gov (United States)

    2010-10-01

    ... River, California, as well as seven artificial propagation programs: the Humboldt Fish Action Council... Basin DPS Sebastes ruberrimus U.S.A.-Washington, and British Columbia, including Puget Sound and Georgia... Basin DPS Sebastes pinniger U.S.A.-Washington, and British Columbia, including Puget Sound and...

  13. CTEPP STANDARD OPERATING PROCEDURE FOR VIDEOTAPING CHILD ACTIVITIES (SOP-2.23)

    Science.gov (United States)

    This SOP describes the method for videotaping a preschool child at a home. The CTEPP main study will collect multimedia samples and questionnaire data at the homes of participants (adults and children) during 48-hr sampling periods. Videotaping the activities of 10% of these chi...

  14. 77 FR 37732 - Fourteenth Meeting: RTCA Special Committee 223, Airport Surface Wireless Communications

    Science.gov (United States)

    2012-06-22

    ... meeting Summary and action item status. General Presentations of interest. ICAO WG-S Status Aloke Roy. WiMAX Forum status WiMAX Forum. MSS interference analysis update NASA. AT4 Wireless analysis...

  15. 76 FR 38740 - Tenth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2011-07-01

    ... General Presentation of Interest Antenna isolation and aircraft installation issues-- Honeywell WiMAX Forum coordination status--WiMAX Forum ] Tuesday Afternoon--MOPS WG Breakout Session Review Status...

  16. 24 CFR 884.223a - Preference for occupancy by elderly families.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Preference for occupancy by elderly... PROGRAM, SECTION 202 SUPPORTIVE HOUSING FOR THE ELDERLY PROGRAM AND SECTION 811 SUPPORTIVE HOUSING FOR... Preference for occupancy by elderly families. (a) Election of preference for occupancy by elderly...

  17. 36 CFR 223.111 - Administration of contracts in designated disaster areas.

    Science.gov (United States)

    2010-07-01

    ...-sharing arrangement in paragraph (a) of this section, he may allow cancellation of the contract... disaster, (2) The sale of such timber will assist in sustaining the economy of such area, or (3) The...

  18. Stellar parameters and chemical abundances of 223 evolved stars with and without planets

    CERN Document Server

    Jofré, E; Saffe, C; Saker, L; de la Villarmois, E Artur; Chavero, C; Gómez, M; Mauas, P

    2014-01-01

    We present fundamental stellar parameters and chemical abundances for a sample of 86 evolved stars with planets and for a control sample of 137 stars without planets. The analysis was based on both high S/N and resolution echelle spectra. The goals of this work are i) to investigate chemical differences between stars with and without planets; ii) to explore potential differences between the properties of the planets around giants and subgiants; and iii) to search for possible correlations between these properties and the chemical abundances of their host stars. In agreement with previous studies, we find that subgiants with planets are, on average, more metal-rich than subgiants without planets by ~ 0.16 dex. The [Fe/H] distribution of giants with planets is centered at slightly subsolar metallicities and there is no metallicity enhancement relative to the [Fe/H] distribution of giants without planets. Furthermore, contrary to recent results, we do not find any clear difference between the metallicity distrib...

  19. The Gln223Arg polymorphism in the leptin receptor is associated with familial combined hyperlipidemia.

    NARCIS (Netherlands)

    Vleuten, G.M. van der; Kluijtmans, L.A.J.; Hijmans, A.G.M.; Blom, H.J.; Stalenhoef, A.F.H.; Graaf, J. de

    2006-01-01

    OBJECTIVE: Familial combined hyperlipidemia (FCH) is characterized by elevated levels of total cholesterol (TC), triglycerides (TG) and apolipoprotein B (apo B) and is associated with premature cardiovascular disease (CVD). Other features of FCH are obesity and insulin resistance. Serum leptin level

  20. 36 CFR 223.52 - Market-related contract term additions.

    Science.gov (United States)

    2010-07-01

    ... AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Contract Conditions and... paragraph (a)(3) of this section, each timber sale contract exceeding 1 year in length shall contain a... the Forest Service has determined that adverse wood products market conditions have resulted in...

  1. 76 FR 72996 - Eleventh Meeting: RTCA Special Committee 223 Airport Surface Wireless Communications

    Science.gov (United States)

    2011-11-28

    ... Chap 8--Physical Layer--Updates per WiMAX Forum Chap 5--Service Specific CS Chap 6--Media Access Control December 7, 2011 MOPS WG Breakout Session Discussion of Security Sub-layer--Honeywell Review...

  2. 78 FR 13395 - Meeting: RTCA Special Committee 223, Airport Surface Wireless Communications

    Science.gov (United States)

    2013-02-27

    ... Meeting Summary. Profile CCB Status. Detailed MOPS Review: Convergence Sub-layer. Security. MAC Layer. Physical Layer. PICS. CRSL. Review/Approval of MOPS for FRAC. Review of Meeting Summary Report. ]...

  3. 36 CFR 223.117 - Administration of cooperative or Federal sustained yield units.

    Science.gov (United States)

    2010-07-01

    ... and by regulation. (b) May offer for sale to cooperators, without competition but at not less than... redetermined rates for stumpage and sale area betterment shall not be less than the base rates in the published... timber for sale to responsible operators within such community or communities, at not less than...

  4. VizieR Online Data Catalog: Hα emitters at z=2.23 (Matsuda+, 2011)

    Science.gov (United States)

    Matsuda, Y.; Smail, I.; Geach, J. E.; Best, P. N.; Sobral, D.; Tanaka, I.; Nakata, F.; Ohta, K.; Kurk, J.; Iwata, I.; Bielby, R.; Wardlow, J. L.; Bower, R. G.; Ivison, R. J.; Kodama, T.; Yamada, T.; Mawatari, K.; Casali, M.

    2012-04-01

    The three target fields (2QZ cluster, 0200+015 and SSA 13) were observed between 2006 May and 2010 April with WFCAM on UKIRT, using the K band and H2S1 filters. To check contamination in our emitter sample from foreground and background line emitters, we obtained B- and z -band images of the target fields with Subaru/Subaru Prime Focus Camera (Suprime-Cam). We observed the 2QZ cluster field in 2009 November and the 0200+015 field in 2010 November. For the SSA 13 field, we used archival data. The 0200+015 field was also observed with the Ks band and H2S1 filters using Subaru/Multi-Object Infrared Camera and Spectrograph (MOIRCS) in 2007 August as part of engineering tests (performed by IT). In addition, we carried out a long-slit spectroscopic observation of a bright emitter candidate in the 0200+015 field with Keck/Low Resolution Imaging Spectrometer (LRIS) in 2010 September. (3 data files).

  5. China, Russia and the Balance of Power in Central Asia. Strategic Forum, Number 223, November 2006

    Science.gov (United States)

    2006-11-01

    migrant workers from the former Soviet countries, including Central Asia. While the exact statistics on migrant labor are difficult to come by...media reports suggest that this traffic has developed into a two-way dependency— Russia on migrant labor from Central Asia, and Central Asia on migrant

  6. 33 CFR 223.1 - Mississippi River Water Control Management Board.

    Science.gov (United States)

    2010-07-01

    ..., responsibilities and authority of the Mississippi River Water Control Management Board. (b) Applicability. This...) Composition. The Mississippi River Water Control Management Board is a continuing board consisting of the... improving inter-divisional coordination of water control management activities within the Mississippi...

  7. Yeast Interacting Proteins Database: YBR223C, YDR510W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available onds, involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in hu...-phosphotyrosyl bonds, involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II

  8. 50 CFR 223.206 - Exceptions to prohibitions relating to sea turtles.

    Science.gov (United States)

    2010-10-01

    ... to prohibitions relating to sea turtles. (a) Permits—(1) Scientific research, education, zoological... zoological exhibition, or to enhance the propagation or survival of threatened species of sea turtles, in... of sea turtle is found injured, dead, or stranded, any agent or employee of the National...

  9. Yeast Interacting Proteins Database: YGR223C, YOR089C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available micronucleophagy; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localiz...old as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Rows with this bait as bait

  10. 75 FR 50867 - Airworthiness Directives; Airbus Model A330-223, -321, -322, and -323 Airplanes

    Science.gov (United States)

    2010-08-18

    ... (PW) forward (FWD) engine mount pylon bolts. * * * * * Dual-bolt fractures could lead to inability for mount assembly to sustain loads which may lead to an engine mount failure and consequently to engine... one of the Pratt & Whitney (PW) forward (FWD) engine mount pylon bolts. Investigation has...

  11. 75 FR 16696 - Airworthiness Directives; Airbus Model A330-223, -321, -322, and -323 Airplanes

    Science.gov (United States)

    2010-04-02

    ... of the Pratt & Whitney (PW) forward (FWD) engine mount pylon bolts. Dual-bolt fractures could lead to inability for mount assembly to sustain loads which may lead to an engine mount failure and consequently to... shank filet radius in one of the Pratt & Whitney (PW) forward (FWD) engine mount pylon...

  12. Sampling for Phase I of the IGE Evaluation. Working Paper No. 223. Report.

    Science.gov (United States)

    Price, Gary G.

    This paper is one of a group that describes the research methodology for an evaluation of individually guided education (IGE) in American elementary schools. Described here are the procedures used to select the sample of schools being studied in the first phase of the evaluation. This phase was a large sample study that used structural equations…

  13. 32 CFR Appendix A to Part 223 - Procedures for Identifying and Controlling DoD UCNI

    Science.gov (United States)

    2010-07-01

    ... or the theft, diversion, or sabotage of DoD SNM, equipment, or facilities. 3. Government information... Government contracts or subcontracts. 3. A person not authorized routine access to DoD UCNI under paragraph C... access is a Federal Government employee, a member of the U.S. Armed Forces, or an employee of a...

  14. 32 CFR Appendix B to Part 223 - Guidelines for the Determination of DoD UCNI

    Science.gov (United States)

    2010-07-01

    ... of nuclear weapons or the theft, diversion, or sabotage of SNM, equipment, or facilities. B. General... an adversary to defeat a security measure; i.e., fire, safety, nuclear accident, radiological release... Weapon Stockpile and Storage Requirement, Nuclear Weapon Destruction and Disablement Systems, and...

  15. 48 CFR 252.223-7002 - Safety precautions for ammunition and explosives.

    Science.gov (United States)

    2010-10-01

    ... component part of an explosive, an ammunition or explosive end item, or of a weapon system— (i) Inert...) Oxidizers; (v) Powdered metals; or (vi) Other materials having fire or explosive characteristics. (b)...

  16. 18 CFR 367.2230 - Account 223, Advances from associate companies.

    Science.gov (United States)

    2010-04-01

    ... associate companies. (a) This account must include the face value of notes payable to associate companies... includible in account 233, Notes payable to associate companies (§ 367.2330), or account 234, Accounts payable to associate companies (§ 367.2340). (b) The records supporting the entries to this account...

  17. Yeast Interacting Proteins Database: YLR223C, YOR247W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available n that exhibits a tight association with the cell wall, required for cell wall stability in the absence...tight association with the cell wall, required for cell wall stability in the absence of GPI-anchored mannop

  18. 2-[2-(3-Chlorophenylhydrazinylidene]-1,3-diphenylpropane-1,3-dione

    Directory of Open Access Journals (Sweden)

    Carlos Bustos

    2011-06-01

    Full Text Available The molecular structure of the title compound, C21H15ClN2O2, features one strong intramolecular N—H...O resonance-assisted hydrogen bond (RAHB. In the crystal, molecules form inversion-related dimers via pairs of weak intermolecular N—H...O contacts. These dimers are further stabilized via three weak C—H...O contacts, developing the three-dimensional structure.

  19. Iu. Ushakov & A. Stukalov, Front voennykh prokurorov [The Military Procurators’ Front], Moskva, Viatka, 2000, 223 pages.

    Directory of Open Access Journals (Sweden)

    Vanessa Voisin

    2008-07-01

    Full Text Available Front voennykh prokurorov is a collection of essays written by two writers who are not known as specialists of military history, but obviously have access to archives, as proves the reprint, in the middle of the book, of several pages from the personal files of Afanas’ev, former Main Military Prosecutor of Soviet Armed Forces.The first part of the book is devoted to Nikolai Porfir’evich Afanas’ev’s memoirs, written, according to the editors, after his retirement in 1950. Afanas’ev, though les...

  20. 36 CFR 223.201 - Limitations on unprocessed timber harvested in Alaska.

    Science.gov (United States)

    2010-07-01

    ... Alaska. Unprocessed timber from National Forest System lands in Alaska may not be exported from the... export of timber, consideration will be given to, among other things, whether such export will: (a... minor species of little importance to local industrial development, or (e) Provide material required...

  1. 36 CFR 223.219 - Sustainable harvest of special forest products.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Sustainable harvest of....219 Sustainable harvest of special forest products. (a) Sustainable harvest levels. Prior to offering... product's sustainable harvest level. A special forest product's sustainable harvest level is the...

  2. 76 FR 20436 - Ninth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2011-04-12

    ... Status RTCA PMC Feedback and Guidance on WiMAX Forum Test Suites General Presentations of Interest Aero... chairmen, members of the public may present oral statements at the meeting. Persons wishing to...

  3. A Taxonomic Approach to Measuring Achievement in Mathematics 223 - Geometry for Elementary Teachers.

    Science.gov (United States)

    Little, Richard A.

    The purpose was to evaluate the effectiveness of a geometry course for preservice elementary teachers and, at the same time, to validate the assumptions that the arrangement of categories in Bloom's "Taxonomy" is hierarchical and cumulative. Sixty-two preservice elementary education majors took an investigator-constructed achievement test after…

  4. International Bathymetric Chart of the Arctic Ocean, Version 2.23

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The goal of this initiative is to develop a digital data base that contains all available bathymetric data north of 64 degrees North, for use by mapmakers,...

  5. 49 CFR Appendix A to Part 223 - Certification of Glazing Materials

    Science.gov (United States)

    2010-10-01

    ... inches in back of the Target Material. The Witness Plate shall have at least an area which will cover the full map of the Target Material. (6) The Witness Plate shall be an unbacked sheet of maximum 0.006 inch... penetration of the back surfaces (side closest to Witness Plate) of the Target Material by the...

  6. 48 CFR 1852.223-75 - Major breach of safety or security.

    Science.gov (United States)

    2010-10-01

    ... major breach of safety may constitute a breach of contract that entitles the Government to exercise any of its rights and remedies applicable to material parts of this contract, including termination for default. A major breach of safety must be related directly to the work on the contract. A major breach...

  7. 46 CFR 10.223 - Modification or removal of limitations or scope.

    Science.gov (United States)

    2010-10-01

    .... (iv) The mandatory requirements for STCW endorsements are contained in parts 11 and 12 of this chapter and in the STCW Convention and Code (incorporated by reference, see § 10.103). (4) The appropriate fee as set forth in § 10.219 of this part; and (5) Any uncanceled MMD, MMC, license, STCW endorsement,...

  8. Bacteria distribution and drug-resistance of 223 isolated enterococci in clinical specimens%223株常见肠球菌的临床分布和耐药性分析

    Institute of Scientific and Technical Information of China (English)

    曾军荣; 李榕娇; 黄绰妤

    2009-01-01

    目的 分析常见肠球菌在临床标本中的分布及对常用抗菌药物的耐药性,为临床治疗提供参考.方法 采用法国梅里埃生物API鉴定系统进行菌种鉴定,并采用K-B纸片扩散法进行体外耐药监测及统计耐药率.结果 肠球菌的临床分离率为8.78%,分类以粪肠球菌为主,其次为屎肠球菌,肠球菌属对利福平、四环素、红霉素、达福普汀、氯霉素耐药率较高.而对万古霉素、替考拉宁和呋喃妥因仍然保持良好的敏感性.粪肠球菌对青霉素、氨苄西林、左旋氧氟沙星的耐药率分别为2.4%、2.4%、5.9%,均低于屎肠球菌的90.7%、88.9%、13.0%,屎肠球菌对四环索、氯霉素的耐药率分别为51.9%和48.1%,均低于粪肠球菌的95.3%和78.1%.结论 临床标本中,肠球菌在泌尿生殖道标本中的分布最高,粪肠球菌和屎肠球菌的耐药谱明显不同,在重症感染时可选用万古霉素、替考拉宁和呋喃妥因进行治疗.%Objective To investigate the distribution and resistance of common Enterococci isolated from clinical specimens in our hospital and to apply some suggestions for treating infections to the clinic.Methods The microbiological system API was used to identify bacteria and the drug-resistance in vitro was determined by K-B methods.Results The clinical isolation rate of enterococci was 8.78%. The main strain of enterococci was Enterococcus faecalis. Enterococcus faecium was the second one.The drug-resistance rate of Enterococci to Rifampin,Tetracycline,Erythromycin,Dalfopristin,Chloramphenicol was high.But it was still sensitive to Vancomycin,Teicoplanin,Nitrofurantoin.The resistance rate of Enterococcus faecalis to Penicillin,Ampicillin,Levofloxacin were2.4%,2.4% and 5.9% respectively .They were lower than Enterococcus faecium (90.7%, 88.9% and 13.0%); The resistance rate of Enterococcus faecium to tetracycline,chloramphenicol were 51.9%,48.1 %. Which were lower than the Enterococcus faecalis 95. 3%,78.1%.Conclusions The distribution rate of Enterococcus spp in genitourinary tract specimens is the highest.Resistance of Enterococcus faecalis and Enterococcus faecium is different.We should choose vanc.omycin,teicoplanin or Nitrofurantoin in severe infection.

  9. Practices And Opinions On In-Center Food Consumption Across 1,223 Facilities In The United States

    OpenAIRE

    Deborah Benner; Marcia Davis; Maria Stasios; Mary Burgess

    2012-01-01

    In the United States, the practices and opinions of healthcare providers regarding eating on dialysis are unknown. The purpose of this study was to understand healthcare provider opinions and in-center hemodialysis patient practices regarding eating while at the dialysis center. In June 2011, over 1200 registered dietitians within a large dialysis organization in the US were surveyed on current practices and opinions of patient food consumption during dialysis treatment using an online survey...

  10. 48 CFR 52.223-9 - Estimate of Percentage of Recovered Material Content for EPA-Designated Items.

    Science.gov (United States)

    2010-10-01

    ... recovered material content for EPA-designated item(s) delivered and/or used in contract performance... responsible for the performance of this contract and hereby certify that the percentage of recovered material content for EPA-designated items met the applicable contract specifications or other...

  11. Aquastress D2.2.3 - Key research priorities for and major knowledge gaps in Water Stress mitigation

    OpenAIRE

    Moors, E; Manez, M.; Van den Wyngaert, I.; Bauer, M; Inman, D.; Koundouri, P.; Loubier, S.; Preziosi, E.; Tarnacki, K.

    2008-01-01

    In this report the factual analysis is compared with the stakeholder perceptions for different test sites. Based on these findings the following general conclusions could be drawn. To obtain a good insight in the bio-geophysical system as well as the socio-economic setting often data were inadequate. This made it difficult to assess the water stress. It also stresses the need for a good monitoring network. A number of sites indicated that the distribution of the amount of water over the diffe...

  12. 75 FR 4477 - Airworthiness Directives; Airbus Model A330-201, -202, -203, -223, -243, -301, -302, -303, -321...

    Science.gov (United States)

    2010-01-28

    ... damage to protective treatments, and deformation of the bogie beam surface of the bogie beam subassembly... and, in case deformation or damage is detected, to apply the associated repair. The one-time... the sliding piston subassembly. Inspection for cracking and deformation of the top and bottom...

  13. Practices And Opinions On In-Center Food Consumption Across 1,223 Facilities In The United States

    Directory of Open Access Journals (Sweden)

    Deborah Benner

    2012-06-01

    The top reasons for facility practices that allowed eating during dialysis were: prevention of hypoglycemia on dialysis, improved kcal intake on dialysis days, and the opportunity to provide counseling on food products currently chosen by the patient. The top reasons for facility practices not permitting eating during dialysis included: potential adverse events associated with hypotension, GI symptoms, choking, infection, pest control, and spills. Further analyses are warranted to determine whether there is a correlation between allowing patients to eat during dialysis treatment and an improvement in the nutritional status of the patients.

  14. 36 CFR 223.64 - Appraisal on a lump-sum value or rate per unit of measure basis.

    Science.gov (United States)

    2010-07-01

    ... equivalent specifications for the following reasons: (a) Variations in lumber or other product value indices... cutting of the timber; (b) Variance between advertised rates and rates redetermined by appraisal at dates... costs or selling values subsequent to the rate redetermination which reduce conversion value to...

  15. 78 FR 59624 - Guidance for Industry #223: Small Entity Compliance Guide-Declaring Color Additives in Animal...

    Science.gov (United States)

    2013-09-27

    .... FDA issued the regulation in response to the Nutrition Labeling and Education Act of 1990 (the 1990 amendments), which amended the Federal Food, Drug, and Cosmetic Act (FD&C Act). Specifically, FDA amended...

  16. Archeological Data Recovery of the Camino Site (16JE223), A Spanish Colonial Period Site Near New Orleans, Louisiana

    Science.gov (United States)

    1996-03-01

    appears to belong to the sedge family ( Cyperaceae ), closely resembling members of the genus Carer. The five seeds from the Camino site are faintly...Alternanthera philoxeroides), sedge (CCyperus odo- ratus), cat-tail (Typha spp.), water millet (Echinochloa walteri), water hyssop (Bacopa mon- nieri...season, they eat leaves, stems, flowers of grasses, herbs, and sedges . They are most active at night and during the early morning. Swamp. Aquatic animals

  17. Cruise report NOAA ship Miller Freeman R-223 during the period of October 11 through November 7, 1981

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This was an opportunity to become more familiar with the wintering ecology of insular fauna of the Aleutian Islands as well as the pelagic fauna of the Bering Sea....

  18. Crystal structure of 2-(2,3-dimethoxynaphthalen-1-yl-3-hydroxy-6-methoxy-4H-chromen-4-one

    Directory of Open Access Journals (Sweden)

    Seunghyun Ahn

    2015-11-01

    Full Text Available In the title compound, C22H18O6, the dimethoxy-substituted naphthalene ring system is twisted relative to the 4H-chromenon skeleton by 88.96 (3°. The two methoxy substituents are tilted from the naphthalene ring system by 1.4 (4 and 113.0 (2°, respectively. An intramolecular O—H...O hydrogen bond closes an S(5 ring motif. In the crystal, pairs of O—H...O hydrogen bonds form inversion dimers with R22(10 loops and C—H...O interactions connect the dimers into [010] chains.

  19. 12 CFR 223.42 - What covered transactions are exempt from the quantitative limits, collateral requirements, and...

    Science.gov (United States)

    2010-01-01

    ... identifiable and publicly available market quotation if the asset's price is quoted routinely in a widely... defined in 17 CFR 240.15c3-1(c)(11)(i); (2) The security is eligible for a State member bank to purchase... asset; (4) The member bank does not purchase the security during an underwriting, or within 30 days...

  20. 48 CFR 952.223-76 - Conditional payment of fee or profit-safeguarding restricted data and other classified...

    Science.gov (United States)

    2010-10-01

    ...., policy, WS&H programs). (viii) Contractor demonstration that an Operating Experience and Feedback Program... contract award include negative monetary incentives in the contract for Contractor violations relating to... failures of similar import will be deemed first degree: (i) Type A accident (defined in DOE Order...

  1. 48 CFR 952.223-77 - Conditional payment of fee or profit-protection of worker safety and health.

    Science.gov (United States)

    2010-10-01

    ... allocation) and to support DOE corporate decision-making (e.g., policy, WS&H programs). (viii) Contractor... Contracting Officer will at the time of contract award include negative monetary incentives in the contract... failures of similar import will be deemed first degree: (i) Type A accident (defined in DOE Order...

  2. 75 FR 50871 - Airworthiness Directives; Airbus Model A330-201, -202, -203, -223, -243, -301, -302, -303, -321...

    Science.gov (United States)

    2010-08-18

    .... Revised Service Information Airbus has issued All Operators Telexes (AOTs) A330-34A3235 (for Model A330... Table 1 of this AD. Table 1--Airbus Service Information Airbus all operators telex-- Revision-- Dated... Information Airbus all operators telex-- Revision-- Dated-- A330-34A3235 Original September 10, 2009....

  3. Intermolecular gold-catalyzed diastereo- and enantioselective [2+2+3] cycloadditions of 1,6-enynes with nitrones.

    Science.gov (United States)

    Gawade, Sagar Ashok; Bhunia, Sabyasachi; Liu, Rai-Shung

    2012-07-27

    Going for gold: The title reaction has been developed and demonstrates a wide substrate scope with respect to the 1,6-enynes and nitrones (see scheme; DCE = 1,2-dichloroethane, Tf = trifluoromethanesulfonyl). The results for the enantioselective versions are also presented.

  4. 75 FR 60655 - Airworthiness Directives; Airbus Model A330-201, -202, -203, -223, and -243 Airplanes; Airbus...

    Science.gov (United States)

    2010-10-01

    ... Inspection Requirements'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic... power unit (APU) bleed leak detection system could result in overheat of the fuel tank located in...

  5. 76 FR 432 - Airworthiness Directives; Airbus Model A330-201, -202, -203, -223, and -243 Airplanes; Airbus...

    Science.gov (United States)

    2011-01-05

    ... October 1, 2010 (75 FR 60655). That NPRM proposed to correct an unsafe condition for the specified...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have... definition review against explosion hazards. * * * * * Failure of the auxiliary power unit (APU) bleed...

  6. 2-[2-(3-Chlorophenyl-2-oxoethyl]-1,2-benzisothiazol-3(2H-one 1,1-dioxide

    Directory of Open Access Journals (Sweden)

    Masood Parvez

    2010-03-01

    Full Text Available In the title compound, C15H10ClNO4S, the benzothiazole ring system is essentially planar [maximum deviation = 0.0382 (13 Å for the N atom] and forms a dihedral angle of 74.43 (6° with the chloro-substituted benzene ring. In the crystal structure, weak intermolecular C—H...O hydrogen bonds form R22(10 and R22(16 ring motifs

  7. 2-[2-(3-Methoxyphenyl-2-oxoethyl]-1,2-benzisothiazol-3(2H-one 1,1-dioxide

    Directory of Open Access Journals (Sweden)

    Masood Parvez

    2010-03-01

    Full Text Available In the title compound, C16H13NO5S, the benzothiazole unit is essentially planar [maximum deviation = 0.0501 (10 Å for the S atom] and is oriented at a dihedral angle of 67.85 (5° with respect to the methoxy-substituted benzene ring. The mean plane of the methoxy group is oriented at 14.3 (3° with respect to the benzene ring to which it is attached. In the crystal structure, weak C—H...O hydrogen bonds form macrocyclic rings with R22(10 and R22(12 motifs.

  8. Review of Andrés Solimano, International Migration in the Age of Crisis and Globalisation. Historical and Recent Experiences, Cambridge University Press, 2010. 223 Pp.

    Directory of Open Access Journals (Sweden)

    Dirina Claudiu – Ciprian

    2015-12-01

    Full Text Available “International Migration in the Age of Crisis and Globalization. Historical and Recent Experiences” represents a work of major interest in the field of migration and globalization. Apparently two concepts that relate one to another, on a background of major population dynamics, the two notions are the two important pillars in what might perfectly be described as a complex analysis of migration, starting from the elements that have initiated it, and culminating with a fine comparison of positive and negative aspects of this phenomenon.

  9. {4,5-Dimethoxy-2-[(2,3-η-2-prop-2-en-1-yl]phenyl-κC1}(8-hydroxyquinolinato-κN,Oplatinum(II

    Directory of Open Access Journals (Sweden)

    Thi Yen Hang Bui

    2016-01-01

    Full Text Available The crystal structure of the organoplatinum(II title complex, [Pt(C9H6NO(C11H13O2] or [Pt(methyleugenol(8-hydroxyquinolinato], has been determined in order to verify the coordination environment of the PtII cation, which was found to be square-planar with the N and O atoms of the quinolinate ligand cis and trans, respectively, with respect to the ethylenic double bond. The least-squares planes through the two aromatic ring systems make an angle of 39.87 (10°. In the crystal, chains are formed parallel to [100] sustained by C—H...O hydrogen bonds. Parallel chains further interact via C—H...O and C—H...π contacts. The complex shows interesting activity on four human cancer cell lines with IC50 values between 1.92 and 4.86 µM.

  10. SU-E-J-223: A BOLD Contrast Imaging Sequence to Evaluate Oxygenation Changes Due to Breath Holding for Breast Radiotherapy: A Pilot Study

    Energy Technology Data Exchange (ETDEWEB)

    Adamson, J; Chang, Z; Cai, J; Palta, M; Horton, J; Yin, F; Blitzblau, R [Duke University Medical Center, Durham, NC (United States)

    2015-06-15

    Purpose: To develop a robust MRI sequence to measure BOLD breath hold induced contrast in context of breast radiotherapy. Methods: Two sequences were selected from prior studies as candidates to measure BOLD contrast attributable to breath holding within the breast: (1) T2* based Gradient Echo EPI (TR/TE = 500/41ms, flip angle = 60°), and (2) T2 based Single Shot Fast Spin Echo (SSFSE) (TR/TE = 3000/60ms). We enrolled ten women post-lumpectomy for breast cancer who were undergoing treatment planning for whole breast radiotherapy. Each session utilized a 1.5T GE MRI and 4 channel breast coil with the subject immobilized prone on a custom board. For each sequence, 1–3 planes of the lumpectomy breast were imaged continuously during a background measurement (1min) and intermittent breath holds (20–40s per breath hold, 3–5 holds per sequence). BOLD contrast was quantified as correlation of changes in per-pixel intensity with the breath hold schedule convolved with a hemodynamic response function. Subtle motion was corrected using a deformable registration algorithm. Correlation with breath-holding was considered significant if p<0.001. Results: The percentage of the breast ROI with positive BOLD contrast measured by the two sequences were in agreement with a correlation coefficient of R=0.72 (p=0.02). While both sequences demonstrated areas with strong BOLD response, the response was more systematic throughout the breast for the SSFSE (T2) sequence (% breast with response in the same direction: 51.2%±0.7% for T2* vs. 68.1%±16% for T2). In addition, the T2 sequence was less prone to magnetic susceptibility artifacts, especially in presence of seroma, and provided a more robust image with little distortion or artifacts. Conclusion: A T2 SSFSE sequence shows promise for measuring BOLD contrast in the context of breast radiotherapy utilizing a breath hold technique. Further study in a larger patient cohort is warranted to better refine this novel technique.

  11. Hyperspectral surface materials map of quadrangle 3670, Jurm-Kishim (223) and Zebak (224) quadrangles, Afghanistan, showing iron-bearing minerals and other materials

    Science.gov (United States)

    King, Trude V.V.; Hoefen, Todd M.; Kokaly, Raymond F.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected iron-bearing minerals and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. This map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Goethite and jarosite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  12. Hyperspectral surface materials map of quadrangle 3670, Jurm-Kishim (223) and Zebak (224) quadrangles, Afghanistan, showing carbonates, phyllosilicates, sulfates, altered minerals, and other materials

    Science.gov (United States)

    Kokaly, Raymond F.; King, Trude V.V.; Hoefen, Todd M.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected carbonates, phyllosilicates, sulfates, altered minerals, and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. The map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Epidote or chlorite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  13. The human RPS4 paralogue on Yq11.223 encodes a structurally conserved ribosomal protein and is preferentially expressed during spermatogenesis

    Directory of Open Access Journals (Sweden)

    Ellis Peter J

    2010-05-01

    Full Text Available Abstract Background The Y chromosome of mammals is particularly prone to accumulate genes related to male fertility. However, the high rate of molecular evolution on this chromosome predicts reduced power to the across-species comparative approach in identifying male-specific genes that are essential for sperm production in humans. We performed a comprehensive analysis of expression of Y-linked transcripts and their X homologues in several human tissues, and in biopsies of infertile patients, in an attempt to identify new testis-specific genes involved in human spermatogenesis. Results We present evidence that one of the primate-specific Y-linked ribosomal protein genes, RPS4Y2, has restricted expression in testis and prostate, in contrast with its X-linked homologue, which is ubiquitously expressed. Moreover, we have determined by highly specific quantitative real time PCR that RPS4Y2 is more highly expressed in testis biopsies containing germ cells. The in silico analysis of the promoter region of RPS4Y2 revealed several differences relative to RPS4Y1, the more widely expressed paralogue from which Y2 has originated through duplication. Finally, through comparative modelling we obtained the three dimensional models of the human S4 proteins, revealing a conserved structure. Interestingly, RPS4Y2 shows different inter-domain contacts and the potential to establish specific interactions. Conclusions These results suggest that one of the Y-linked copies of the ribosomal protein S4 is preferentially expressed during spermatogenesis and might be important for germ cell development. Even though RPS4Y2 has accumulated several amino acid changes following its duplication from RPS4Y1, approximately 35 million years ago, the evolution of the Y-encoded RPS4 proteins is structurally constrained. However, the exclusive expression pattern of RPS4Y2 and the novelties acquired at the C-terminus of the protein may indicate some degree of functional specialisation of this protein in spermatogenesis.

  14. 47 CFR 25.223 - Off-axis EIRP spectral density limits for feeder link earth stations in the 17/24 GHz BSS.

    Science.gov (United States)

    2010-10-01

    .... The amount of such increase in excess of the actual amount of monitored excess attenuation over clear....209(a) and (b), and/or the proposed power density levels are in excess of those specified in § 25.212... this part, each applicant for earth station license(s) that proposes levels in excess of those...

  15. 2016年底Facebook全球用户将逾14亿人广告收入达223.7亿美元

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    eMarketer预计2016年是Facebook用户增长速度维持两位数的最后一年,到2016年底Facebook用户将达到14.3亿人。同时预测到2020年,Face.book月活跃用户将达18.7亿人。

  16. The Gln223Arg polymorphism of the leptin receptor in Pima Indians: influence on energy expenditure, physical activity and lipid metabolism

    DEFF Research Database (Denmark)

    Stefan, N; Vozarova, B; Del Parigi, A

    2002-01-01

    whether this variant influences energy metabolism and adiposity in Pima Indians, we genotyped non-diabetic Pima Indians in whom we had measured body composition and 24 h energy expenditure (24 h EE), physical activity level (PAL) and 24 h respiratory quotient (24 h RQ) in a respiratory chamber (n=268...

  17. Amplification of chromosome 2:Lq22.3 harboring trefoil factor family genes in liver fluke related cholangiocarcinoma is associated with poor prognosis

    Institute of Scientific and Technical Information of China (English)

    Kanuengnuch Muenphon; Temduang Limpaiboon; Patcharee Jearanaikoon; Chawalit Pairojkul; Banchob Sripa; Vajarabhongsa Bhudhisawasdi

    2006-01-01

    AIM: To determine allelic imbalance on chromosomal region 21q22-qter including trefoil factor family genes (TFF) in cholangiocarcinoma (CCA) patients and analyze the correlation between allelic imbalances and clinicopathological parameters.METHODS: Quantitative PCR amplification was performed on four microsatellite markers and trefoil factor family genes (TFF1, TFF2, and TFF3) using a standard curve and SYBR Green Ⅰ dye method. The relative copy number was determined by DNA copy number of tested locus to reference locus. The relative copy number was interpreted as deletion or amplification by comparison with normal reference range. Associations between allelic imbalance and clinicopathological parameters of CCA patients were evaluated by x2-tests.Kaplan-Meier method was used to analyze survival.RESULTS: The frequencies of amplification at D21S1890,D21S1893, and TFF3 were 32.5%, 30.0%, and 28.7%,respectively. Patients who had amplification at regions covering D21S1893, D21S1890, and TFF showed poor prognosis, whereas patients who had deletion showed favorable prognosis (mean: 51.7 wk vs 124.82 wk,P = 0.012). Multivariate Cox regression analysis revealed that amplification of D21S1893, D21S1890 and TFF,blood vessel invasion, and staging were associated with poor prognosis.CONCLUSION: D21S1893-D21S1890 region may harbor candidate genes especially TFF and serine protease family, which might be involved in tumor invasion and metastasis contributing to poor survival. The amplification in this region may be used as a prognostic marker in the treatment of CCA patients.

  18. Submarine groundwater discharge revealed by radium isotopes (Ra-223 and Ra-224 near a paleochannel on the Southern Brazilian continental shelf

    Directory of Open Access Journals (Sweden)

    Karina Kammer Attisano

    2013-09-01

    Full Text Available Submarine Groundwater Discharge (SGD has been recognized as an important component of the ocean-continent interface. The few previous studies in Brazil have focused on nearshore areas. This paper explores SGD on the Southern Brazilian Continental Shelf using multiple lines of evidence that include radium isotopes, dissolved nutrients, and water mass observations. The results indicated that SGD may be occurring on the Continental Shelf in the Albardão region, near a paleochannel located 50 km offshore. This paleochannel may thus be a preferential pathway for the delivery of nutrient- and metal-enriched groundwater and porewater into continental shelf waters.

  19. 10 CFR Appendix E to Part 20 - Nationally Tracked Source Thresholds

    Science.gov (United States)

    2010-01-01

    ... Category 1(TBq) Category 1(Ci) Category 2(TBq) Category 2(Ci) Actinium-227 20 540 0.2 5.4 Americium-241 60... 2 54 Strontium-90 1,000 27,000 10 270 Thorium-228 20 540 0.2 5.4 Thorium-229 20 540 0.2 5.4...

  20. Monofunctionalization of Calix[4]arene Tetracarboxylic Acid at the Upper Rim with Isothiocyanate Group: First Bifunctional Chelating Agent for Alpha-Emitter Ac-225.

    Science.gov (United States)

    Chen, Xiaoyuan; Ji, Min; Fisher, Darrell R; Wai, Chien M

    1999-09-01

    A procedure is reported for synthesizing a novel, water-soluble bifunctional chelating agent derived from calix[4]arene. This chelate features tetracarboxylic acid groups at the lower rim as an actinium-225 ionophore, and an isothiocyanate functional group at the upper rim for labeling of the N-terminus of monoclonal antibodies through thiourea linkage.

  1. I. The metabolic properties of plutonium and allied materials

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, J.G.

    1948-05-24

    This report on the metabolic properties of plutonium and related radioactive materials presents experimental information in the following areas: radioautographic studies; tracer studies (with tables of accumulation in tissues) of actinium, radio-zirconium, technetium, radio-rubidium, radio-germanium, beryllium, and cadmium; decontamination and bone metabolism studies; and radio-chemical isolation.

  2. New production in the South China Sea

    Institute of Scientific and Technical Information of China (English)

    CAI; Pinghe

    2002-01-01

    [1]Dugdale, R. C., Goering, J. J., Uptake of new and regenerated nitrogen in primary productivity, Limnology and Oceanog-raphy, 1967, 12: 196-206.[2]Eppley, R. W., Peterson, B. J., Particulate organic matter flux and planktonic new production in the deep ocean, Nature, 1979, 282: 677-680.[3]Allen, C. B., Kanda, J., Laws, E. A., New production and photosynthetic rates within and outside a cyclonic mesoscale eddy in the North Pacific Subtropical gyre, Deep-Sea Research Ⅱ, 1996, 43(6): 917-936.[4]Jenkins, W. J., Tritium-helium dating in the Sargasso Sea: A measurement of oxygen utilization rates, Nature, 1977, 196: 291-292.[5]Spitzer, W. S., Jenkins, W. J., Rates of vertical mixing, gas exchange and new production: Estimates from seasonal gas cy-cle in the upper ocean near Bermuda, Journal of Marine Research, 1989, 47: 169-196.[6]Sarmiento, J. L., Thiele, G., Key, R. M. et al., Oxygen and nitrate new production and remineralization in the North At-lantic Subtropical Gyre, Journal of Geophysical Research, 1990, 95: 18303-18315.[7]Ku, T. L., Luo, S., Kusakabe, M. et al., 228Ra-derived nutrient budgets in upper equatorial Pacific and the role of "new" silicate in limiting productivity, Deep-Sea Research II, 1995, 42(2/3): 479-497.[8]Wheeler, P. A., Rates of nitrate assimilation at the equator during the US-JGOFS spring time series: Estimates of new pro-duction, EOS, Transactions, American Geophysical Union, 1992, 73: 281.[9]Huang, Y., Xie, Y., Chen, M. et al., Distribution feature of 228Ra in surface seawater of the Nansha sea area, in Isotopes Marine Chemistry of Nansha Islands Waters (in Chinese), Beijing: Ocean Press, 1996, 70-78.[10]Reid, D. F., Key, R. M., Schink, D., Radium, thorium and actinium extraction from seawater using an improved manga-nese-oxide-coated fiber, Earth and Planetary Science Letters, 1979, 43: 223-226.[11]Shi, W., Qiu, X., Huang, Y., Distribution of dissolved 226Ra in the Jiulongjiang-Xiamen bay

  3. Study of the origin of elements of the uranium-235 family observed in excess in the vicinity of the experimental nuclear EL4 reactor under dismantling. Lessons got at this day and conclusions; Etude de l'origine des elements de la famille de l'uranium-235 observes en exces dans les environs du reacteur nucleaire experimental EL4 en cours de demantelement. Enseignements retires a ce jour et conclusion

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This study resumes the discovery of an excess of actinium 227 found around by EL4 nuclear reactor actually in dismantling. The search for the origin of this excess revealed a real inquiry of investigation during three years. Because a nuclear reactor existed in this area a particular attention will have concerned this region. The doubt became the line of conduct to find the answer to the human or natural origin of this excess. Finally and against any evidence, it appears that the origin of this phenomenon was natural, consequence of the particular local geology. The detail of the different investigations is given: search of a possible correlation with the composition of elevations constituent of lanes, search (and underlining) of new sites in the surroundings of the Rusquec pond and the Plouenez station, study of the atmospheric deposits under winds of the nuclear power plant and in the east direction, search of a possible relationship with the gaseous effluents of the nuclear power plant in the past, historical study of radioactive effluents releases in the fifty last years by the analysis of the sedimentary deposits in the Saint-Herbiot reservoir, search of a possible correlation between the excess of actinium 227 and the nuclear power plant activity; search of a possible correlation with a human activity without any relationship with the nuclear activities, search of a correlation with the underground waters, search of a correlation with the geological context, collect of information on the possible transfers in direction of the food chain, determination of the radiological composition of the underground waters ( not perturbed by human activity), search of the cause of an excess of actinium 227 in the old channel of liquid effluents release of the nuclear power plant. The results are given and discussed. And contrary to all expectations the origin of the excess of actinium 227 is completely natural. (N.C.)

  4. Behaviour of uranium series radionuclides in surface water (Crouzille, Limousin). Geochemical implications; Comportement des radionucleides des familles de l'uranium dans les eaux superficielles du site de la Crouzille (Limousin): implications geochimiques

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-06-15

    Understanding natural radionuclides behaviour in surface water is a required step to achieve uranium mine rehabilitation and preserve water quality. The first objective of this thesis is to determine which are the radionuclides sources in a drinking water reservoir. The second objective is to improve the knowledge about the behaviour of uranium series radionuclides, especially actinium. The investigated site is a brook (Sagnes, Limousin, France) which floods a peat bog contaminated by a former uranium mine and which empties into the Crouzille lake. It allows studying radionuclides transport in surface water and radionuclides retention through organic substance or water reservoir. Radionuclides distribution in particulate, colloidal and dissolved phases is determined thanks to ultra-filtrations. Gamma spectrometry allows measuring almost all natural radionuclides with only two counting stages. However, low activities of {sup 235}U series radionuclides impose the use of very low background well-type Ge detectors, such as those of the Underground Laboratory of Modane (France). Firstly, this study shows that no or few radionuclides are released by the Sagnes peat bog, although its radioactivity is important. Secondly, it provides details on the behaviour of uranium series radionuclides in surface water. More specifically, it provides the first indications of actinium solubility in surface water. Actinium's behaviour is very close to uranium's even if it is a little less soluble. (author)

  5. CALABI, Francesca. Fílon de Alexandria (Filone di Alessandria. Tradução: José Bortolini. São Paulo: Paulus, 2014. 223 pp. ISBN: 978-85-349-3854-9. R$ 38,00.

    Directory of Open Access Journals (Sweden)

    Rodrigo Pinto de Brito

    2016-08-01

    Full Text Available Francesca Calabi é professora do Dipartamento di Studi Umanistici – Filosofia na Università degli Studi di Pavia, na Itália, onde leciona desde 2004 disciplinas de História da Filosofia Tardo-antiga. Seus cursos versam sobre temas como “O daimon de Sócrates” e “O asno de ouro” (ambas obras de Apuleio. Também lê, revisa e finaliza traduções suas em sala de aula, como a da “Carta de Aristeas a Filócrates”, um texto do séc. III a.C que descreve a tradução da Torá para o grego pelos 72 sábios e tradutores enviados a Alexandria a convite do então bibliotecário alexandrino, o peripatético Demétrio de Falero.

  6. Alterations in candidate genes PHF2, FANCC, PTCH1 and XPA at chromosomal 9q22.3 region: Pathological significance in early- and late-onset breast carcinoma

    Directory of Open Access Journals (Sweden)

    Roychoudhury Susanta

    2008-11-01

    Full Text Available Abstract Introduction Younger women with breast carcinoma (BC exhibits more aggressive pathologic features compared to older women; young age could be an independent predictor of adverse prognosis. To find any existing differences in the molecular pathogenesis of BC in both younger and older women, alterations at chromosomal (chr. 9q22.32-22.33 region were studied owing to its association in wide variety of tumors. Present work focuses on comparative analysis of alterations of four candidate genes; PHF2, FANCC, PTCH1 and XPA located within 4.4 Mb region of the afore-said locus in two age groups of BC, as well as the interrelation and prognostic significance of alterations of these genes. Methods Deletion analysis of PHF2, FANCC, PTCH1 and XPA were examined in a subset of 47 early-onset (group-A: ≤ 40 years and 59 late-onset (group-B: > 40 years breast carcinomas using both microsatellite and exonic markers. Methylation Sensitive Restriction analysis (MSRA was done to check for promoter methylation. Quantitative real-time polymerase chain reaction (Q-PCR and immunohistochemisty (IHC was done in some genes to see their relative mRNA and protein expressions respectively. Clinico-pathological correlation of different parameters as well as patient survival was calculated using different statistical softwares like EpiInfo 6.04b, SPSS 10.0 etc. Results Either age group exhibited high frequency of overall alterations in PHF2, FANCC and PTCH1 compared to XPA. Samples with alteration (deletion/methylation in these genes showed reduced level of mRNA expression as seen by Q-PCR. Immunohistochemical analysis of FANCC and PTCH1 also supported this observation. Poor patient survival was noted in both age groups having alterations in FANCC. Similar result was also seen with PTCH1 and XPA alterations in group-A and PHF2 alterations in group-B. This reflected their roles as prognostic tools in the respective groups in which they were altered. Conclusion Overall alterations of PHF2, FANCC and PTCH1 were comparatively higher than XPA. Differential association of alterations in FANCC and PTCH1 with that of PHF2, XPA and two breast cancer susceptibility genes (BRCA1/BRCA2 in the two age groups suggests differences in their molecular pathogenesis and dysregulation of multiple DNA repair pathways as well as hedgehog dependent stem cell renewal pathway.

  7. Gestire l’errore e l’uso della lingua madre nella classe di lingua.DOI: 10.5007/2175-7968.2011v1n27p223

    Directory of Open Access Journals (Sweden)

    Paolo Torresan

    2011-11-01

    Full Text Available L’uso della lingua madre e la correzione dell’errore sono state questioni rimaste a lungo in ombra nella storia della didattica delle lingue. È stato come se nella pratica docente, negli ultimi anni in cui è invalso l’approccio comunicativo, la questione si risolvesse in due assiomi: la lingua madre va limitata il più possibile, in quanto l’uso rallenterebbe -se non addirittura impedirebbe- la crescita del parlante in lingua straniera e, poiché all’accuratezza va preferita la fluenza, è opportuno l’errore sia corretto soprattutto – se non addirittura solamente – quando rende difficile la comunicazione. In questo saggio, la rassegna delle posizioni assunte da vari esperti di educazione linguistica, alcune delle quali si collocano ai margini dell’approccio comunicativo, dimostra come in realtà la questione è tutt’altro che semplice e numerose sono le scelte metodologiche di cui l’insegnante può disporre. The issues of mother tongue usage and error correction have long remained poorly elucidated in the history of language teaching. In recent years, during which the communicative approach has become established, the issue has appeared to resolve according to two precepts: mother tongue should be limited as far as possible, given that its usage is thought to slow – or even hinder – the student’s progress in the foreign language and, since fluency is preferred over accuracy, errors should above all – or indeed only – be corrected when they impede communication. This discussion compiles the stances taken by a number of language education experts, some of whom position themselves on the margins of the communicative approach, demonstrating that, in reality, the issue is far from simple and there are a wide array of methodological choices available for teachers.

  8. Association of breast cancer risk in BRCA1 and BRCA2 mutation carriers with genetic variants showing differential allelic expression: identification of a modifier of breast cancer risk at locus 11q22.3

    NARCIS (Netherlands)

    Y. Hamdi (Yosr); Soucy, P. (Penny); Kuchenbaeker, K.B. (Karoline B.); Pastinen, T. (Tomi); A. Droit (Arnaud); Lemaçon, A. (Audrey); J.W. Adlard (Julian); K. Aittomäki (Kristiina); I.L. Andrulis (Irene); A. Arason (Adalgeir); N. Arnold (Norbert); B.K. Arun (Banu); J. Azzollini; A.L. Bane (Anita L.); Barjhoux, L. (Laure); D. Barrowdale (Daniel); J. Benítez (Javier); P. Berthet (Pascaline); M.J. Blok (Marinus); K.A. Bobolis (Kristie A.); V. Bonadona (Valérie); B. Bonnani (Bernardo); Bradbury, A.R. (Angela R.); C. Brewer (Carole); B. Buecher (Bruno); Buys, S.S. (Saundra S.); M.A. Caligo (Maria); Chiquette, J. (Jocelyne); W. Chung (Wendy); K.B.M. Claes (Kathleen B.M.); Daly, M.B. (Mary B.); F. Damiola (Francesca); R. Davidson (Rosemarie); M. de La Hoya (Miguel); K. De Leeneer (Kim); O. Díez (Orland); Y.C. Ding (Yuan); R. Dolcetti (Riccardo); S.M. Domchek (Susan); C.M. Dorfling (Cecilia); D. Eccles (Diana); R. Eeles (Ros); Z. Einbeigi (Zakaria); B. Ejlertsen (Bent); EMBRACE; C. Engel (Christoph); Gareth Evans, D.; L. Feliubadaló (L.); L. Foretova (Lenka); F. Fostira (Florentia); Foulkes, W.D. (William D.); G. Fountzilas (George); E. Friedman (Eitan); D. Frost (Debra); P. Ganschow (Pamela); P.A. Ganz (Patricia A.); J. Garber (Judy); S.A. Gayther (Simon); GEMO Study Collaborators; A-M. Gerdes (Anne-Marie); G. Glendon (Gord); A.K. Godwin (Andrew K.); D. Goldgar (David); M.H. Greene (Mark H.); J. Gronwald (Jacek); E. Hahnen (Eric); U. Hamann (Ute); T.V.O. Hansen (Thomas); S. Hart (Stewart); J. Hays (John); HEBON; F.B.L. Hogervorst (Frans); P.J. Hulick (Peter); E.N. Imyanitov (Evgeny); C. Isaacs (Claudine); L. Izatt (Louise); A. Jakubowska (Anna); M. James (Margaret); R. Janavicius (Ramunas); U.B. Jensen; E.M. John (Esther); V. Joseph (Vijai); Just, W. (Walter); Kaczmarek, K. (Katarzyna); Karlan, B.Y. (Beth Y.); KConFab Investigators; C.M. Kets; J. Kirk (Judy); Kriege, M. (Mieke); Y. Laitman (Yael); Laurent, M. (Maïté); C. Lazaro (Conxi); Leslie, G. (Goska); K.J. Lester (Kathryn); F. Lesueur (Fabienne); A. Liljegren (Annelie); N. Loman (Niklas); J.T. Loud (Jennifer); S. Manoukian (Siranoush); Mariani, M. (Milena); S. Mazoyer (Sylvie); L. McGuffog (Lesley); E.J. Meijers-Heijboer (Hanne); A. Meindl (Alfons); A. Miller (Austin); M. Montagna (Marco); A.-M. Mulligan (Anna-Marie); K.L. Nathanson (Katherine); S.L. Neuhausen (Susan); H. Nevanlinna (Heli); R.L. Nussbaum (Robert L.); Olah, E. (Edith); O.I. Olopade (Olufunmilayo I.); K.-R. Ong (Kai-Ren); J.C. Oosterwijk (Jan); A. Osorio (Ana); L. Papi (Laura); S.K. Park (Sue K.); Pedersen, I.S. (Inge Sokilde); B. Peissel (Bernard); P.P. Segura (Pedro Perez); P. Peterlongo (Paolo); C. Phelan (Catherine); P. Radice (Paolo); J. Rantala (Johanna); Rappaport-Fuerhauser, C. (Christine); G. Rennert (Gad); A.L. Richardson (Andrea); M. Robson (Mark); G.C. Rodriguez (Gustavo); M.A. Rookus (Matti); R.K. Schmutzler (Rita); N. Sevenet (Nicolas); Shah, P.D. (Payal D.); C.F. Singer (Christian); Slavin, T.P. (Thomas P.); Snape, K. (Katie); J. Sokolowska (Johanna); Sønderstrup, I.M.H. (Ida Marie Heeholm); M.C. Southey (Melissa); A.B. Spurdle (Amanda); Stadler, Z. (Zsofia); D. Stoppa-Lyonnet (Dominique); G. Sukiennicki (Grzegorz); C. Sutter (Christian); Tan, Y. (Yen); M.-K. Tea; P.J. Teixeira; A. Teulé (A.); S.-H. Teo; M.B. Terry (Mary Beth); M. Thomassen (Mads); L. Tihomirova (Laima); M. Tischkowitz (Marc); S. Tognazzo (Silvia); A.E. Toland (Amanda); N. Tung (Nadine); A.M.W. van den Ouweland (Ans); R.B. van der Luijt (Rob); K. van Engelen (Klaartje); E.J. van Rensburg (Elizabeth); R. Varon-Mateeva (Raymonda); B. Wapenschmidt (Barbara); J.T. Wijnen (Juul); R. Rebbeck (Timothy); G. Chenevix-Trench (Georgia); K. Offit (Kenneth); Couch, F.J. (Fergus J.); S. Nord (Silje); D.F. Easton (Douglas F.); A.C. Antoniou (Antonis C.); Simard, J. (Jacques)

    2016-01-01

    textabstractPurpose: Cis-acting regulatory SNPs resulting in differential allelic expression (DAE) may, in part, explain the underlying phenotypic variation associated with many complex diseases. To investigate whether common variants associated with DAE were involved in breast cancer susceptibility

  9. Crystal structure of chlorido{4,5-dimethoxy-2-[(2,3-η-2-prop-2-en-1-yl]phenyl-κC1}(piperidine-κNplatinum(II ethanol monosolvate

    Directory of Open Access Journals (Sweden)

    Peter Mangwala Kimpende

    2014-11-01

    Full Text Available In the title compound, [Pt(C11H13O2Cl(C5H11N]·C2H5OH, the PtII cation is pentacoordinated in a distorted square-planar geometry. In the crystal, inversion dimers showing C—H...Cl and C—H...π interactions are further stacked in columns along the a axis via C—H...π interactions. The ethanol solvate molecule interacts with neighbouring methoxy groups of methyleugenol through O—H...O hydrogen bonds.

  10. Efeitos da restrição energética para suínos na fase final de terminação sobre o desempenho, característica de carcaça e poluição ambiental - DOI: 10.4025/actascianimsci.v29i2.223 Effect of energy restriction for late finishing pigs on performance, carcass traits and environmental pollution - DOI: 10.4025/actascianimsci.v29i2.223

    Directory of Open Access Journals (Sweden)

    Antonio Cláudio Furlan

    2007-11-01

    Full Text Available . Foram conduzidos dois experimentos com o objetivo de avaliar níveis de restrição energética (3.200, 2.960 e 2.720 kcal de EM kg-1, por meio da elevação da fibra dietéica (casca de arroz, para suínos abatidos com peso elevado. Experimento 1 - Foram utilizados 36 suínos (69,39 ± 5,38 kg. As variáveis estudadas (desempenho, carcaça e NUP foram agrupadas de acordo com o peso de abate (70 - 90 kg e 90 - 115 kg. Ao todo, foram abatidos 12 animais com peso de 87,55 ± 3,12 kg e 15 animais com peso de 115,41 ± 6,20 kg. Nenhuma das variáveis estudadas foi influenciada pelos diferentes níveis de EM. Experimento 2 - Foram utilizados 15 suínos machos (84,8 ± 4,5 kg, alojados em gaiolas de metabolismo. Houve aumento linear da produção total de fezes, matéria seca e matéria orgânica, com a redução do nível energético, contudo sem aumentar a excreção total de fósforo (P e nitrogênio (N. Houve redução do coeficiente de digestibilidade da MS e PB das raízes com a redução da energia (elevação da fibra, o que levou a redução do GDP e piora na CA. Os resultados sugerem que o abate de suínos com peso de até 115 kg é possível sem prejuízo do desempenho e das características da carcaça, independente do nível de EM das dietas, entretanto ocorre aumento na produção total de fezes com a redução da EM e aumento da fibra dietética sem, contudo, aumentar a excreção de N e P.Two trials were carried out to study the energy restriction (3200, 2960 and 2720 kcal of ME kg-1 for late finishing pigs, by means of adding dietary fiber (ground rice hull. Experiment I - 36 pigs were used (69.39 ア 5.38 kg. The variables studied (performance, carcass traits and PUN were separated according to the slaughter weight (70-90 kg and 90-115 kg. 12 pigs weighting 87.55 ア 3.12 kg and 15 pigs weighting 115.41 ア 6.20 kg were slaughtered. None of the variables studied were influenced by the different levels of ME. Experiment II -15 barrows were used (84.8 ア 4.5 kg and kept on metabolism crates. There was a linear increase of total feces, dry matter (DM and organic matter output, according to dietary energy reduction, without causing an increase in total phosphorous (P and nitrogen (N excretion. Digestibility coefficient of DM and crude protein decreased, which led to a decrease in daily weight gain and an increase in feed: gain ratio. Results suggest the possibility to slaughter high lean meat pigs, weighting up to 115 kg, without impairing performance and carcass traits, regardless dietary energy level. However, total feces output increased without causing an increase in N and P excretion, as ME decreased and crude fiber increased.

  11. 12 CFR 218.760 - Exemption from definition of “broker” for banks accepting orders to effect transactions in...

    Science.gov (United States)

    2010-01-01

    ... any Web site, newspaper, magazine or other periodical, radio, television, telephone or tape recording... as defined in section 223(d) of the Internal Revenue Code (26 U.S.C. 223(d)), Archer medical...

  12. Pharmacological characterisation of α6β4* nicotinic acetylcholine receptors assembled from three different α6/α3 subunit chimeras in tsA201 cells

    DEFF Research Database (Denmark)

    Jensen, Anne Bjørnskov; Hoestgaard-Jensen, Kirsten; Jensen, Anders A.

    2014-01-01

    . In the FLIPR™ Membrane Potential Blue assay, the agonists exhibited the same rank order of potencies [(±)-epibatidine>sazetidine A>varenicline>(−)-cytisine~(S)-nicotine>acetylcholine>carbachol] at the C1β4, C1β4β3, C6F223Lβ4, C6F223Lβ4β3, C16F223Lβ4 and C16F223Lβ4β3 receptors, albeit the absolute EC50 values...

  13. 76 FR 14592 - Surety Companies Doing Business With the United States

    Science.gov (United States)

    2011-03-17

    ... Fiscal Service 31 CFR Part 223 RIN 1510-AB27 Surety Companies Doing Business With the United States... companies doing business with the United States. When a Treasury-certified surety cedes non-Federal risks to..., we propose to amend 31 CFR part 223 as set forth below: PART 223--SURETY COMPANIES DOING...

  14. 75 FR 35517 - Summary of Precedent Opinions of the General Counsel

    Science.gov (United States)

    2010-06-22

    ... Appeals for Veterans Claims (Veterans Court) in Osborn v. Nicholson, 21 Vet. App. 223 (2007), that... of Osborn v. Nicholson, 21 Vet. App. 223 (2007), that interest received from the redemption of a... Osborn v. Nicholson, 21 Vet. App. 223 (2007), conflicts with VAOPGCPREC 1-93, in which we held that:...

  15. DISSOLVED CONCENTRATION LIMITS OF RADIOACTIVE ELEMENTS

    Energy Technology Data Exchange (ETDEWEB)

    P. Bernot

    2005-07-13

    The purpose of this study is to evaluate dissolved concentration limits (also referred to as solubility limits) of elements with radioactive isotopes under probable repository conditions, based on geochemical modeling calculations using geochemical modeling tools, thermodynamic databases, field measurements, and laboratory experiments. The scope of this activity is to predict dissolved concentrations or solubility limits for elements with radioactive isotopes (actinium, americium, carbon, cesium, iodine, lead, neptunium, plutonium, protactinium, radium, strontium, technetium, thorium, and uranium) relevant to calculated dose. Model outputs for uranium, plutonium, neptunium, thorium, americium, and protactinium are provided in the form of tabulated functions with pH and log fCO{sub 2} as independent variables, plus one or more uncertainty terms. The solubility limits for the remaining elements are either in the form of distributions or single values. Even though selection of an appropriate set of radionuclides documented in Radionuclide Screening (BSC 2002 [DIRS 160059]) includes actinium, transport of Ac is not modeled in the total system performance assessment for the license application (TSPA-LA) model because of its extremely short half-life. Actinium dose is calculated in the TSPA-LA by assuming secular equilibrium with {sup 231}Pa (Section 6.10); therefore, Ac is not analyzed in this report. The output data from this report are fundamental inputs for TSPA-LA used to determine the estimated release of these elements from waste packages and the engineered barrier system. Consistent modeling approaches and environmental conditions were used to develop solubility models for the actinides discussed in this report. These models cover broad ranges of environmental conditions so they are applicable to both waste packages and the invert. Uncertainties from thermodynamic data, water chemistry, temperature variation, and activity coefficients have been quantified or

  16. Analysis of the Yrast Bands with q-Deformed Moment of Inertia

    Institute of Scientific and Technical Information of China (English)

    FANG Xiang-Zheng; RUAN Tu-Nan

    2001-01-01

    The rigid-rotor with a q-deformed moment of inertia is introduced to describe the nuclear rotational spectra. With the representations of quantum algebra, the normal deformed and superdeformed bands are naturally differentiated by softness. The yrast normal deformed bands in rare earth and actinium regions, as well as the yrast superdeformed bands in A-190 and 150 regions are investigated. The calculated results agree with experimental data qualitatively well, and the values of the parameters are physically reasonable. This indicates that the fixed deformation, the stretching effect and the many body statistics effect are three possible dominant factors to govern nuclear rotational bands.

  17. Transition wavelengths and unresolved transition array statistics of ions with Z = 72-89

    Energy Technology Data Exchange (ETDEWEB)

    Kilbane, D, E-mail: Deirdre.Kilbane@ucd.ie [School of Physics, University College Dublin, Belfield, Dublin 4 (Ireland)

    2011-08-28

    Potential extreme ultraviolet and soft x-ray radiation sources have been identified, using the flexible atomic code (FAC), as emission peaks arising from the 4d-4f and 4p-4d transitions in Pd-like to Rb-like ions of hafnium through actinium. The effects of configuration interaction are investigated and for increasing nuclear charge, these strong emitters are seen to separate and move to shorter wavelengths. Each source is characterized using the unresolved transition array model. They are proposed to complement the currently used nitrogen and argon sources in the 'water window', and as possible successors to tin in next-generation lithography.

  18. Detection of rare earth elements in Powder River Basin sub-bituminous coal ash using laser-induced breakdown spectroscopy (LIBS)

    Energy Technology Data Exchange (ETDEWEB)

    Tran, Phuoc [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State; Mcintyre, Dustin [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State

    2015-10-01

    We reported our preliminary results on the use of laser-induced breakdown spectroscopy to analyze the rare earth elements contained in ash samples from Powder River Basin sub-bituminous coal (PRB-coal). We have identified many elements in the lanthanide series (cerium, europium, holmium, lanthanum, lutetium, praseodymium, promethium, samarium, terbium, ytterbium) and some elements in the actinide series (actinium, thorium, uranium, plutonium, berkelium, californium) in the ash samples. In addition, various metals were also seen to present in the ash samples

  19. Purification of cerium, neodymium and gadolinium for low background experiments

    Directory of Open Access Journals (Sweden)

    Boiko R.S.

    2014-01-01

    Full Text Available Cerium, neodymium and gadolinium contain double beta active isotopes. The most interesting are 150Nd and 160Gd (promising for 0ν2β search, 136Ce (2β+ candidate with one of the highest Q2β. The main problem of compounds containing lanthanide elements is their high radioactive contamination by uranium, radium, actinium and thorium. The new generation 2β experiments require development of methods for a deep purification of lanthanides from the radioactive elements. A combination of physical and chemical methods was applied to purify cerium, neodymium and gadolinium. Liquid-liquid extraction technique was used to remove traces of Th and U from neodymium, gadolinium and for purification of cerium from Th, U, Ra and K. Co-precipitation and recrystallization methods were utilized for further reduction of the impurities. The radioactive contamination of the samples before and after the purification was tested by using ultra-low-background HPGe gamma spectrometry. As a result of the purification procedure the radioactive contamination of gadolinium oxide (a similar purification efficiency was reached also with cerium and neodymium oxides was decreased from 0.12 Bq/kg to 0.007 Bq/kg in 228Th, from 0.04 Bq/kg to <0.006 Bq/kg in 226Ra, and from 0.9 Bq/kg to 0.04 Bq/kg in 40K. The purification methods are much less efficient for chemically very similar radioactive elements like actinium, lanthanum and lutetium.

  20. The Technological Enhancement of Normally Occurring Radioactive Materials in Red Mud due to the Production of Alumina

    Directory of Open Access Journals (Sweden)

    Maurice O. Miller

    2016-01-01

    Full Text Available This study investigates the level of technological enhancement of normally occurring radioactive materials (TENORM in the red mud waste due to the production of alumina in Jamaica. Technological enhancements factors (TEF were determined for the uranium, thorium, actinium series, their progenies, and the nonseries potassium-40 using gamma spectrometry. The study concluded that bauxite production technologically enhances the uranium progenies Th-234, Pb-214, Bi-214, and Pa-234 and the thorium-232 progenies Ac-228, Pb-212, and Bi-212 in red mud. The actinium series was technologically enhanced, but K-40 and the thorium daughter, Tl-208, were reduced. The spectrometric comparison of Tl-208 (at 510 keV was unexpected since its other photopeaks at 583 keV, 934 keV, and 968 keV were markedly different. An explanation for this anomaly is discussed. An explanation regarding the process of accumulation and fractionation of organically derived phosphate deposits and potassium-feldspar is offered to explain the spectrometric differences between the alumina product and its waste material, red mud.

  1. University Students Are Unaware of the Role of Academic Librarians. A Review of: Bickley, R. & Corral, S. (2011. Student perceptions of staff in the information commons: A survey at the University of Sheffield. Reference Services Review, 39(2, 223-243. doi:10.1108/00907321111135466

    Directory of Open Access Journals (Sweden)

    Kirsty Thomson

    2012-06-01

    Full Text Available Objective – To discover students’ perceptionsof information commons staff, and todetermine how these perceptions influence theuse of library resources.Design – Post-experience survey with onefollow-up interview.Setting – The University of Sheffield, a postsecondaryinstitution in England.Subjects – All undergraduate andpostgraduate students were invited to takepart. Just over 1% of the student population, or250 students, completed the survey.Methods – Information about the survey wassent to students’ institutional email addresses.One follow up interview was carried out viaemail using the critical incident technique.Main Results – Students do not understandthe academic roles of librarians. They areunlikely to approach library staff for academicsupport, preferring to turn to instructors, otherstudents, friends, and family. Most studentshad positive opinions about assistancereceived in the Information Commons, but asmall number reflected on previous badexperiences with staff, or on a fear of beingmade to feel foolish. The vast majority ofstudents who did not seek help in theInformation Commons stated that this wasbecause they did not require assistance. Most students do not perceive a difference between Information Commons staff and library staff.Conclusion – Students have positive views of Information Commons staff at the University of Sheffield, but have low awareness of the roles of professional librarians. Librarians need to develop partnerships with academic staff and strengthen their presence in both physical and online learning environments to promote their academic roles.

  2. Corrigendum to "Evolution of a Neoproterozoic suture in the Iberian Massif, Central Portugal: New U-Pb ages of igneous and metamorphic events at the contact between the Ossa Morena Zone and Central Iberian Zone" [Lithos 220-223 (2015) 43-59

    Science.gov (United States)

    Henriques, S. B. A.; Neiva, A. M. R.; Ribeiro, M. L.; Dunning, G. R.; Tajčmanová, L.

    2016-11-01

    The Authors regret to have inadvertently omitted an important grant number in the Acknowledgement section of the published article. The following sentence should be added to the end of Acknowledgement:

  3. Multifrequency observations of extended radio galaxies V - 3C 31, 3C 33.1, 3C 35, 3C 66B, 3C 129, 3C 130, 3C 223, 3C 310, 3C 390.3 and 4C 48.29

    Science.gov (United States)

    van Breugel, W.; Jagers, W.

    1982-08-01

    A sample of 3C radio sources of large angular size has been observed in total and polarized intensity at several wavelengths with the Westerbork Synthesis Radio Telescope. The sources were selected such that their largest angular size was greater than about 200 arcsec and their declination greater than about 25 degrees. Some additional sources with radio jets or peculiar morphology were also included. The name of each source, its structural type classification, wavelength of observation, and data references are given.

  4. (1R*,21S*,22R*,24S*-Methyl ethyl 2-[23-hydroxy-22,24-diphenyl-8,11,14-trioxa-25-azatetracyclo[19.3.1.02,7.015,20]pentacosa-2,4,6,15(20,16,18-hexaen-25-yl]but-2-enedioate

    Directory of Open Access Journals (Sweden)

    Truong Hong Hieu

    2013-07-01

    Full Text Available The title compound, C40H41NO8, is a product of the reduction of the cyclic carbonyl group of the γ-piperidone subunit of the aza-14-crown-4 ether with subsequent re-esterification of its dimethyl butenoate substituent into a monoethyl monomethyl group. The azacrown macrocycle exhibits a bowl conformation with a dihedral angle of 70.82 (5° between the benzene rings fused to it. The piperidine ring adopts a chair conformation and the methyl ethyl ethylenedicarboxylate fragment has a cis conformation, with a dihedral angle of 66.51 (7° between the two carboxylate groups. The ethyl group is disordered over two sites with occupancies of 0.70 (1:0.30 (1. In the crystal, molecules form inversion dimers, via pairs of O—H...O hydrogen bonds, that stack along the a axis.

  5. Dicty_cDB: SLI504 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available update 1999. 2.23 Translated Amino Acid sequence ffffflllfyn*lfllifi*t*iftnfinskrngnswdnfkisrndtmiesnqtfi**...l Frames) Frame A: ffffflllfyn*lfllifi*t*iftnfinskrngnswdnfkisrndtmiesnqtfi**df l

  6. Recombinant DNA technology for melanoma immunotherapy: anti-Id DNA vaccines targeting high molecular weight melanoma-associated antigen.

    Science.gov (United States)

    Barucca, A; Capitani, M; Cesca, M; Tomassoni, D; Kazmi, U; Concetti, F; Vincenzetti, L; Concetti, A; Venanzi, F M

    2014-11-01

    Anti-idiotypic MK2-23 monoclonal antibody (anti-Id MK2-23 mAb), which mimics the high molecular weight melanoma-associated antigen (HMW-MAA), has been used to implement active immunotherapy against melanoma. However, due to safety and standardization issues, this approach never entered extensive clinical trials. In the present study, we investigated the usage of DNA vaccines as an alternative to MK2-23 mAb immunization. MK2-23 DNA plasmids coding for single chain (scFv) MK2-23 antibody were constructed via the insertion of variable heavy (V H) and light (V L) chains of MK2-23 into the pVAC-1mcs plasmids. Two alternative MK2-23 plasmids format V H/V L, and V L/V H were assembled. We demonstrate that both polypeptides expressed by scFv plasmids in vitro retained the ability to mimic HMW-MAA antigen, and to elicit specific anti-HMW-MAA humoral and cellular immunoresponses in immunized mice. Notably, MK2-23 scFv DNA vaccines impaired the onset and growth of transplantable B16 melanoma cells not engineered to express HMW-MAA. This pilot study suggests that optimized MK2-23 scFv DNA vaccines could potentially provide a safer and cost-effective alternative to anti-Id antibody immunization, for melanoma immunotherapy.

  7. Unigene BLAST: CBRC-CELE-04-0088 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CELE-04-0088 gnl|UG|Cel#S5808591 Caenorhabditis elegans Seven TM Receptor fami...ly member (str-223) (str-223) mRNA, complete cds /cds=p(1,1029) /gb=NM_064280 /gi=17534202 /ug=Cel.26909 /len=1029 9e-67 39% ...

  8. 10 CFR Appendix C to Part 20 - Quantities 1 of Licensed Material Requiring Labeling

    Science.gov (United States)

    2010-01-01

    ... Polonium-205 1,000 Polonium-207 1,000 Polonium-210 0.1 Astatine-207 100 Astatine-211 10 Radon-220 1 Radon-222 1 Francium-222 100 Francium-223 100 Radium-223 0.1 Radium-224 0.1 Radium-225 0.1 Radium-226...

  9. 36 CFR 261.6 - Timber and other forest products.

    Science.gov (United States)

    2010-07-01

    ... of 1990 (16 U.S.C. 620, et seq.), or its implementing regulations at 36 CFR 223.185-223.203... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Timber and other forest products. 261.6 Section 261.6 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF...

  10. 75 FR 52912 - Airworthiness Directives; Bell Helicopter Textron Canada Limited Model 427 Helicopters

    Science.gov (United States)

    2010-08-30

    ... driveshaft hanger bearing bracket part number (P/N) 427-044-223-101 has been found cracked due to fatigue. It.... FOR FURTHER INFORMATION CONTACT: Sharon Miles, Aerospace Engineer, FAA, Rotorcraft Directorate, 2601... driveshaft hanger bearing bracket part number (P/N) 427-044-223-101 has been found cracked due to fatigue....

  11. GETDB: 113490 [GETDB

    Lifescience Database Archive (English)

    Full Text Available of leg, wing cns internal - P{GawB}NP4661 comment1:A, comment2:23B1-B2 d1 d2 d3 d...internal Lethality - Also known as P{GawB}NP4661 Original Comments comment1:A, comment2:23B1-B2 Disc (Image)

  12. 77 FR 13971 - Regulated Navigation Area; MBTA Saugus River Railroad Drawbridge Rehabilitation Project, Saugus...

    Science.gov (United States)

    2012-03-08

    ... 617-223-4000, email Mark.E.Cutter@uscg.mil or Lieutenant Junior Grade Isaac Slavitt, Coast Guard First District Waterways Management Branch, telephone 617-223- 8385, email Isaac.M.Slavitt@uscg.mil . If you have... will communicate with vessels via VHF-FM radio or loudhailer. Members of the Coast Guard Auxiliary...

  13. 14 CFR 417.111 - Launch plans.

    Science.gov (United States)

    2010-01-01

    ... as required by § 417.223, and identification of procedures for surveillance and clearance of these... procedures for coordinating use of radio frequencies with any launch site operator and any local and Federal... notices to mariners; (iii) Provide hazard area information prepared as required by § 417.223 or §...

  14. 47 CFR 1.80 - Forfeiture proceedings.

    Science.gov (United States)

    2010-10-01

    ...), 219(b), 220(d), 223(b), 362(a), 362(b), 386(a), 386(b), 503(b), 506, and 634 of the Communications Act... unauthorized equipment 7,000 Exceeding of authorized antenna height 5,000 Fraud by wire, radio or television 5... under section 504 of the Act. The one exception is section 223 of the Act, which provides a...

  15. 49 CFR Appendix A to Part 211 - Statement of Agency Policy Concerning Waivers Related to Shared Use of Trackage or Rights-of-Way...

    Science.gov (United States)

    2010-10-01

    .... To the extent radio communication is used to direct the movements, the radio rules (part 220) apply... standards (part 223). This part provides that passenger car windows be equipped with FRA-certified glazing... equivalent safeguards are in place to provide the same assurance as part 223 that passengers and...

  16. 77 FR 24415 - Inflation Adjustment of the Aggravated Maximum Civil Monetary Penalty for a Violation of a...

    Science.gov (United States)

    2012-04-24

    ... Federal Railroad Administration 49 CFR Parts 209, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223...); 49 CFR 240.223(a), (b). Further, FRA is revising language in 49 CFR part 209, appendix A, ``Statement..., Transportation. 49 CFR Part 220 Communications, Penalties, Radio, Railroad safety, Reporting and...

  17. 77 FR 73916 - Regulated Navigation Area; S99 Alford Street Bridge Rehabilitation Project, Mystic River, MA

    Science.gov (United States)

    2012-12-12

    ... 617-223-4000, email Mark.E.Cutter@uscg.mil ; or Lieutenant Isaac Slavitt, Coast Guard First District Waterways Management Branch, telephone 617-223-8385, email Isaac.M.Slavitt@uscg.mil . If you have questions... radio or loudhailer. Members of the Coast Guard Auxiliary may be present to inform vessel operators...

  18. 77 FR 1020 - Regulated Navigation Area; S99 Alford Street Bridge Rehabilitation Project, Mystic River, MA

    Science.gov (United States)

    2012-01-09

    ... Sector Boston Waterways Management Division, telephone (617) 223-4000, email Mark.E.Cutter@uscg.mil ; or...) 223- 8385, email Isaac.M.Slavitt@uscg.mil . If you have questions on viewing the docket, call Renee V... VHF-FM radio or loudhailer. Members of the Coast Guard Auxiliary may be present to inform...

  19. 77 FR 29251 - Safety Zone; Carnival Fireworks Display, Nantasket Beach, Hull, MA

    Science.gov (United States)

    2012-05-17

    .... Mark Cutter, Coast Guard Sector Boston Waterways Management Division, telephone 617-223-4000, email... Management Branch, telephone 617-223- 8385, email Isaac.M.Slavitt@uscg.mil . If you have questions on viewing... by siren, radio, flashing light, or other means, the operator of a vessel shall proceed as...

  20. Carbon-nitrogen cycling in northern Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Naqvi, S.W.A

    stream_size 20 stream_content_type text/plain stream_name IGBP_India_2000_223.pdf.txt stream_source_info IGBP_India_2000_223.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...