WorldWideScience

Sample records for actinium 215

  1. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-08-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac-Cl and Ac-OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1- ligands (3.2+/-1.1) than AmIII (0.8+/-0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour.

  2. Separation of Actinium 227 from the uranium minerals

    International Nuclear Information System (INIS)

    The purpose of this work was to separate Actinium 227, whose content is 18%, from the mineral carnotite found in Gomez Chihuahua mountain range in Mexico. The mineral before processing is is pre-concentrated and passed, first through anionic exchange resins, later the eluate obtained is passed through cationic resins. The resins were 20-50 MESH QOWEX and 100-200 MESH 50 X 8-20 in some cased 200-400 MESH AG 50W-X8, 1X8 in other cases. The eluates from the ionic exchange were electrodeposited on stainless steel polished disc cathode and platinum electrode as anode; under a current ODF 10mA for 2.5 to 5 hours and of 100mA for .5 of an hour. it was possible to identify the Actinium 227 by means of its descendents, TH-227 and RA-223, through alpha spectroscopy. Due to the radiochemical purity which the electro deposits were obtained the Actinium 227 was low and was not quantitatively determined. A large majority of the members of the natural radioactive series 3 were identified and even alpha energies reported in the literature with very low percentages of non-identified emissions were observed. We conclude that a more precise study is needed concerning ionic exchange and electrodeposit to obtain an Actinium 227 of radiochemical purity. (Author)

  3. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-01-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac–Cl and Ac–OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1– ligands (3.2±1.1) than AmIII (0.8±0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour. PMID:27531582

  4. Discovery of the actinium, thorium, protactinium, and uranium isotopes

    CERN Document Server

    Fry, C

    2012-01-01

    Currently, 31 actinium, 31 thorium, 28 protactinium, and 23 uranium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  5. Spectroscopic and computational investigation of actinium coordination chemistry.

    Science.gov (United States)

    Ferrier, Maryline G; Batista, Enrique R; Berg, John M; Birnbaum, Eva R; Cross, Justin N; Engle, Jonathan W; La Pierre, Henry S; Kozimor, Stosh A; Lezama Pacheco, Juan S; Stein, Benjamin W; Stieber, S Chantal E; Wilson, Justin J

    2016-01-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, Ac(III) reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac-Cl and Ac-OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between Ac(III) and Am(III) in HCl solutions indicate Ac(III) coordinates more inner-sphere Cl(1-) ligands (3.2±1.1) than Am(III) (0.8±0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique Ac(III) chemical behaviour. PMID:27531582

  6. IT 215 courses / snaptutorial

    OpenAIRE

    IT 210 snaptutorial

    2015-01-01

    For more classes visit www.snaptutorial.com   IT 215 Week 1 CheckPoint: Text Printing Program   IT 215 Week 1 DQ 1   IT 215 Week 1 DQ 2   IT 215 Week 2 CheckPoint: Payroll Program Part 1   IT 215 Week 2 CheckPoint: Benefits of Programming Knowledge   IT 215 Week 3 CheckPoint: Payroll Program Part 2   IT 215 Week 3 DQ 1   IT 215 Week 3 DQ 2   IT 215 Week 4 CheckPoint: Method...

  7. Production of Actinium-225 via High Energy Proton Induced Spallation of Thorium-232

    International Nuclear Information System (INIS)

    The science of cancer research is currently expanding its use of alpha particle emitting radioisotopes. Coupled with the discovery and proliferation of molecular species that seek out and attach to tumors, new therapy and diagnostics are being developed to enhance the treatment of cancer and other diseases. This latest technology is commonly referred to as Alpha Immunotherapy (AIT). Actinium-225/Bismuth-213 is a parent/daughter alpha-emitting radioisotope pair that is highly sought after because of the potential for treating numerous diseases and its ability to be chemically compatible with many known and widely used carrier molecules (such as monoclonal antibodies and proteins/peptides). Unfortunately, the worldwide supply of actinium-225 is limited to about 1,000mCi annually and most of that is currently spoken for, thus limiting the ability of this radioisotope pair to enter into research and subsequently clinical trials. The route proposed herein utilizes high energy protons to produce actinium-225 via spallation of a thorium-232 target. As part of previous R and D efforts carried out at Argonne National Laboratory recently in support of the proposed US FRIB facility, it was shown that a very effective production mechanism for actinium-225 is spallation of thorium-232 by high energy proton beams. The base-line simulation for the production rate of actinium-225 by this reaction mechanism is 8E12 atoms per second at 200 MeV proton beam energy with 50 g/cm2 thorium target and 100 kW beam power. An irradiation of one actinium-225 half-life (10 days) produces ∼100 Ci of actinium-225. For a given beam current the reaction cross section increases slightly with energy to about 400 MeV and then decreases slightly for beam energies in the several GeV regime. The object of this effort is to refine the simulations at proton beam energies of 400 MeV and above up to about 8 GeV. Once completed, the simulations will be experimentally verified using 400 MeV and 8 GeV protons

  8. Production of Actinium-225 via High Energy Proton Induced Spallation of Thorium-232

    Energy Technology Data Exchange (ETDEWEB)

    Harvey, James T.; Nolen, Jerry; Vandergrift, George; Gomes, Itacil; Kroc, Tom; Horwitz, Phil; McAlister, Dan; Bowers, Del; Sullivan, Vivian; Greene, John

    2011-12-30

    The science of cancer research is currently expanding its use of alpha particle emitting radioisotopes. Coupled with the discovery and proliferation of molecular species that seek out and attach to tumors, new therapy and diagnostics are being developed to enhance the treatment of cancer and other diseases. This latest technology is commonly referred to as Alpha Immunotherapy (AIT). Actinium-225/Bismuth-213 is a parent/daughter alpha-emitting radioisotope pair that is highly sought after because of the potential for treating numerous diseases and its ability to be chemically compatible with many known and widely used carrier molecules (such as monoclonal antibodies and proteins/peptides). Unfortunately, the worldwide supply of actinium-225 is limited to about 1,000mCi annually and most of that is currently spoken for, thus limiting the ability of this radioisotope pair to enter into research and subsequently clinical trials. The route proposed herein utilizes high energy protons to produce actinium-225 via spallation of a thorium-232 target. As part of previous R and D efforts carried out at Argonne National Laboratory recently in support of the proposed US FRIB facility, it was shown that a very effective production mechanism for actinium-225 is spallation of thorium-232 by high energy proton beams. The base-line simulation for the production rate of actinium-225 by this reaction mechanism is 8E12 atoms per second at 200 MeV proton beam energy with 50 g/cm2 thorium target and 100 kW beam power. An irradiation of one actinium-225 half-life (10 days) produces {approx}100 Ci of actinium-225. For a given beam current the reaction cross section increases slightly with energy to about 400 MeV and then decreases slightly for beam energies in the several GeV regime. The object of this effort is to refine the simulations at proton beam energies of 400 MeV and above up to about 8 GeV. Once completed, the simulations will be experimentally verified using 400 MeV and 8 Ge

  9. Comment: 215 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available 215.png Taxonomy icon (c) Database Center for Life Science licensed under CC Attribution2.1 Japan アイコン:電子顕微鏡バージョン bando 2010/02/15 15:30:03 2010/02/15 15:30:03 ...

  10. IT 215 UOP Course Tutorial / Tutorialoutlet

    OpenAIRE

    abilagh

    2015-01-01

    For more course tutorials visit www.tutorialoutlet.com       IT 215 Week 1 CheckPoint: Text Printing Program IT 215 Week 1 Discussion Question 1 IT 215 Week 1 Discussion Question 2 IT 215 Week 2 CheckPoint: Payroll Program Part 1 IT 215 Week 2 CheckPoint: Benefits of Programming Knowledge IT 215 Week 3 CheckPoint: Payroll Program Part 2 IT 215 Week 3 Discussion Question 1 IT 215 Week 3 Discussion Question 2 IT 215 Week 4 Check...

  11. IT 215 UOP Tutorial Courses/ Uoptutorial

    OpenAIRE

    kalam1

    2015-01-01

    For more course tutorials visit www.uoptutorial.com   IT 215 Week 1 CheckPoint: Text Printing Program   IT 215 Week 1 DQ 1   IT 215 Week 1 DQ 2   IT 215 Week 2 CheckPoint: Payroll Program Part 1   IT 215 Week 2 CheckPoint: Benefits of Programming Knowledge   IT 215 Week 3 CheckPoint: Payroll Program Part 2   IT 215 Week 3 DQ 1   IT 215 Week 3 DQ 2   IT 215 Week 4 CheckPoin...

  12. 76 FR 6536 - Airworthiness Directives; Bombardier, Inc. Model CL-215-1A10 (CL-215), CL-215-6B11 (CL-215T...

    Science.gov (United States)

    2011-02-07

    ... products. That NPRM was published in the Federal Register on November 9, 2010 (75 FR 68728). That NPRM... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant.... Model CL-215-1A10 (CL- 215), CL-215-6B11 (CL-215T Variant), and CL-215-6B11 (CL-415 Variant)...

  13. Analysis of the gamma spectra of the uranium, actinium, and thorium decay series

    International Nuclear Information System (INIS)

    This report describes the identification of radionuclides in the uranium, actinium, and thorium series by analysis of gamma spectra in the energy range of 40 to 1400 keV. Energies and absolute efficiencies for each gamma line were measured by means of a high-resolution germanium detector and compared with those in the literature. A gamma spectroscopy method, which utilizes an on-line computer for deconvolution of spectra, search and identification of each line, and estimation of activity for each radionuclide, was used to analyze soil and uranium tailings, and ore

  14. IT 215 UOP COURSE TUTORIAL/ UOPHELP

    OpenAIRE

    RAVIBHARGAVI

    2015-01-01

    For more course tutorials visit www.uophelp.com   IT 215 Week 1 CheckPoint: Text Printing Program   IT 215 Week 1 Discussion Question 1   IT 215 Week 1 Discussion Question 2   IT 215 Week 2 CheckPoint: Payroll Program Part 1   IT 215 Week 2 CheckPoint: Benefits of Programming Knowledge   IT 215 Week 3 CheckPoint: Payroll Program Part 2   IT 215 Week 3 Discussion Question 1   IT 215 Week...

  15. 75 FR 68728 - Airworthiness Directives; Bombardier, Inc. Model CL-215-1A10 (CL-215), CL-215-6B11 (CL-215T...

    Science.gov (United States)

    2010-11-09

    ... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or.... Model CL-215-1A10 (CL- 215), CL-215-6B11 (CL-215T Variant), and CL-215-6B11 (CL-415 Variant) Airplanes...: * * * * * Seven cases of on-ground hydraulic accumulator screw cap or end cap failure have been experienced on...

  16. 48 CFR 215.404 - Proposal analysis.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Proposal analysis. 215.404 Section 215.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... Proposal analysis....

  17. 2 CFR 215.34 - Equipment.

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Equipment. 215.34 Section 215.34 Grants and... ORGANIZATIONS (OMB CIRCULAR A-110) Post Award Requirements Property Standards § 215.34 Equipment. (a) Title to equipment acquired by a recipient with Federal funds shall vest in the recipient, subject to conditions...

  18. 48 CFR 833.215 - Contract clause.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract clause. 833.215 Section 833.215 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS GENERAL CONTRACTING REQUIREMENTS PROTESTS, DISPUTES, AND APPEALS Disputes and Appeals 833.215 Contract clause. The...

  19. 2 CFR 215.43 - Competition.

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Competition. 215.43 Section 215.43 Grants... ORGANIZATIONS (OMB CIRCULAR A-110) Post Award Requirements Procurement Standards § 215.43 Competition. All... free competition. The recipient shall be alert to organizational conflicts of interest as well...

  20. 32 CFR 215.6 - Responsibilities.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Responsibilities. 215.6 Section 215.6 National... EMPLOYMENT OF MILITARY RESOURCES IN THE EVENT OF CIVIL DISTURBANCES § 215.6 Responsibilities. (a) The... the accomplishment of his public affairs responsibilities set forth in paragraph (f) of this...

  1. 32 CFR 215.10 - Funding.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Funding. 215.10 Section 215.10 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS EMPLOYMENT OF MILITARY RESOURCES IN THE EVENT OF CIVIL DISTURBANCES § 215.10 Funding. (a) Reporting requirements...

  2. 7 CFR 65.215 - Pork.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Pork. 65.215 Section 65.215 Agriculture Regulations of..., PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL COMMODITIES, MACADAMIA NUTS, PECANS, PEANUTS, AND GINSENG General Provisions Definitions § 65.215 Pork. Pork means meat produced from hogs....

  3. 47 CFR 80.215 - Transmitter power.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Transmitter power. 80.215 Section 80.215... MARITIME SERVICES General Technical Standards § 80.215 Transmitter power. (a) Transmitter power shown on the radio station authorization is the maximum power the licensee is authorized to use. Power...

  4. 25 CFR 215.12 - Advertising costs.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Advertising costs. 215.12 Section 215.12 Indians BUREAU... LEASES, QUAPAW AGENCY § 215.12 Advertising costs. All advertising costs, publication fees, expenses incurred for abstracts of lease title, and other expenses incurred in connection with the advertising...

  5. 22 CFR 215.2 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Definitions. 215.2 Section 215.2 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT REGULATIONS FOR IMPLEMENTATION OF PRIVACY ACT OF 1974 § 215.2... citizen of the United States or an alien lawfully admitted for permanent residence; (e) Maintain...

  6. 27 CFR 555.215 - Housekeeping.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false Housekeeping. 555.215 Section 555.215 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Storage § 555.215 Housekeeping....

  7. 49 CFR 215.203 - Restricted cars.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Restricted cars. 215.203 Section 215.203..., DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Restricted Equipment § 215.203 Restricted cars. (a) This section restricts the operation of any railroad freight car that is— (1) More than...

  8. 49 CFR 199.215 - Alcohol concentration.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Alcohol concentration. 199.215 Section 199.215... Prevention Program § 199.215 Alcohol concentration. Each operator shall prohibit a covered employee from... alcohol concentration of 0.04 or greater. No operator having actual knowledge that a covered employee...

  9. 31 CFR 800.215 - Foreign national.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Foreign national. 800.215 Section 800.215 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... TAKEOVERS BY FOREIGN PERSONS Definitions § 800.215 Foreign national. The term foreign national means...

  10. 48 CFR 215.270 - Peer Reviews.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Peer Reviews. 215.270 Section 215.270 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... of Proposals and Information 215.270 Peer Reviews. Agency officials shall conduct Peer Reviews...

  11. 32 CFR 215.3 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... EMPLOYMENT OF MILITARY RESOURCES IN THE EVENT OF CIVIL DISTURBANCES § 215.3 Definitions. (a) Civil disturbances are group acts of violence and disorders prejudicial to public law and order within the 50 States... 32 National Defense 2 2010-07-01 2010-07-01 false Definitions. 215.3 Section 215.3...

  12. 14 CFR 121.215 - Cabin interiors.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Cabin interiors. 121.215 Section 121.215 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR CARRIERS..., FLAG, AND SUPPLEMENTAL OPERATIONS Special Airworthiness Requirements § 121.215 Cabin interiors....

  13. ADJ 215 UOP Course Tutorial/TutorialRank

    OpenAIRE

    apj

    2015-01-01

    For more course tutorials visit www.tutorialrank.com Tutorial Purchased: 1 Times, Rating:     ADJ 215 Week 1 Checkpoint Survey of Local Crime Prevention Methods ADJ 215 Week 1 DQ 1 and DQ 2 ADJ 215 Week 2 Assignment Just Desserts ADJ 215 Week 3 DQ 1 and DQ 2 ADJ 215 Week 4 Checkpoint Social Structure Crime ADJ 215 Week 4 Checkpoint Diversion Program ADJ 215 Week 4 Assignment Socialization and Criminal Behavior ADJ 215 Week 5 Checkpoint...

  14. Thorium and actinium polyphosphonate compounds as bone-seeking alpha particle-emitting agents.

    Science.gov (United States)

    Henriksen, Gjermund; Bruland, Oyvind S; Larsen, Roy H

    2004-01-01

    The present study explores the use of alpha-particle-emitting, bone-seeking agents as candidates for targeted radiotherapy. Actinium and thorium 1,4,7,10 tetraazacyclododecane N,N',N'',N''' 1,4,7,10-tetra(methylene) phosphonic acid (DOTMP) and thorium-diethylene triamine N,N',N'' penta(methylene) phosphonic acid (DTMP) were prepared and their biodistribution evaluated in conventional Balb/C mice at four hours after injection. All three bone-seeking agents showed a high uptake in bone and a low uptake in soft tissues. Among the soft tissue organs, only kidney had a relatively high uptake. The femur/kidney ratios for 227Th-DTMP, 228-Ac-DOTMP and 227Th-DOTMP were 14.2, 7.6 and 6.0, respectively. A higher liver uptake of 228Ac-DOTMP was seen than for 227Th-DTMP and 227Th-DOTMP. This suggests that some demetallation of the 228Ac-DOTMP complex had occurred. The results indicate that 225Ac-DOTMP, 227Th-DOTMP and 227Th-DTMP have promising properties as potential therapeutic bone-seeking agents.

  15. Developments towards in-gas-jet laser spectroscopy studies of actinium isotopes at LISOL

    Science.gov (United States)

    Raeder, S.; Bastin, B.; Block, M.; Creemers, P.; Delahaye, P.; Ferrer, R.; Fléchard, X.; Franchoo, S.; Ghys, L.; Gaffney, L. P.; Granados, C.; Heinke, R.; Hijazi, L.; Huyse, M.; Kron, T.; Kudryavtsev, Yu.; Laatiaoui, M.; Lecesne, N.; Luton, F.; Moore, I. D.; Martinez, Y.; Mogilevskiy, E.; Naubereit, P.; Piot, J.; Rothe, S.; Savajols, H.; Sels, S.; Sonnenschein, V.; Traykov, E.; Van Beveren, C.; Van den Bergh, P.; Van Duppen, P.; Wendt, K.; Zadvornaya, A.

    2016-06-01

    To study exotic nuclides at the borders of stability with laser ionization and spectroscopy techniques, highest efficiencies in combination with a high spectral resolution are required. These usually opposing requirements are reconciled by applying the in-gas-laser ionization and spectroscopy (IGLIS) technique in the supersonic gas jet produced by a de Laval nozzle installed at the exit of the stopping gas cell. Carrying out laser ionization in the low-temperature and low density supersonic gas jet eliminates pressure broadening, which will significantly improve the spectral resolution. This article presents the required modifications at the Leuven Isotope Separator On-Line (LISOL) facility that are needed for the first on-line studies of in-gas-jet laser spectroscopy. Different geometries for the gas outlet and extraction ion guides have been tested for their performance regarding the acceptance of laser ionized species as well as for their differential pumping capacities. The specifications and performance of the temporarily installed high repetition rate laser system, including a narrow bandwidth injection-locked Ti:sapphire laser, are discussed and first preliminary results on neutron-deficient actinium isotopes are presented indicating the high capability of this novel technique.

  16. 76 FR 34014 - Airworthiness Directives; Bombardier, Inc. Model CL-215-1A10, CL-215-6B11 (CL-215T Variant), and...

    Science.gov (United States)

    2011-06-10

    ... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or.... Model CL-215-1A10, CL- 215-6B11 (CL-215T Variant), and CL-215-6B11 (CL-415 Variant) Airplanes AGENCY.... Model CL-215-1A10 airplanes, serial numbers 1051 through 1125 inclusive; Model CL-215- 6B11...

  17. Dicty_cDB: SSI215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SS (Link to library) SSI215 (Link to dictyBase) - - - Contig-U12249-1 SSI215Z (Link... to Original site) - - SSI215Z 640 - - - - Show SSI215 Library SS (Link to library) Clone ID SSI215 (Link to...ycdb.biol.tsukuba.ac.jp/CSM/SS/SSI2-A/SSI215Q.Seq.d/ Representative seq. ID SSI21...5Z (Link to Original site) Representative DNA sequence >SSI215 (SSI215Q) /CSM/SS/SSI2-A/SSI215Q.Seq.d/ XXXXX... producing significant alignments: (bits) Value SSI215 (SSI215Q) /CSM/SS/SSI2-A/SSI215Q.Seq.d/ 803 0.0 VSC83

  18. 33 CFR 117.215 - Niantic River.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Niantic River. 117.215 Section... DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Connecticut § 117.215 Niantic River. (a) The draw of the Amtrak bridge, mile 0.0 at Niantic, shall open on signal; except that, from April 1...

  19. 2 CFR 215.24 - Program income.

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Program income. 215.24 Section 215.24 Grants... income. (a) Federal awarding agencies shall apply the standards set forth in this section in requiring recipient organizations to account for program income related to projects financed in whole or in part...

  20. 20 CFR 627.215 - Relocation.

    Science.gov (United States)

    2010-04-01

    ... products or business being transferred; the date the facility will commence or expand operations, and... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Relocation. 627.215 Section 627.215 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR GENERAL PROVISIONS GOVERNING...

  1. 24 CFR 884.215 - Lease requirements.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Lease requirements. 884.215 Section... RENTAL HOUSING PROJECTS Project Development and Operation § 884.215 Lease requirements. The Lease shall... provisions listed in paragraph (c) of this section. (a) Term of lease. The term of the Lease shall be for...

  2. 5 CFR 179.215 - Refunds.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Refunds. 179.215 Section 179.215 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS CLAIMS COLLECTION STANDARDS... (unless expressly prohibited by statute or regulation); or (2) An administrative or judicial order...

  3. 2 CFR 215.31 - Insurance coverage.

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Insurance coverage. 215.31 Section 215.31 Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET CIRCULARS AND GUIDANCE Reserved UNIFORM... Insurance coverage. Recipients shall, at a minimum, provide the equivalent insurance coverage for...

  4. 14 CFR 21.215 - Application.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Application. 21.215 Section 21.215 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT CERTIFICATION... Office in the geographic area in which the manufacturer or air carrier is located. The application...

  5. 48 CFR 233.215 - Contract clause.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contract clause. 233.215....215 Contract clause. Use Alternate I of the clause at FAR 52.233-1, Disputes, when— (1) The... (v) Tracked combat vehicles (vi) Related electronic systems; (2) The contracting officer...

  6. 7 CFR 215.3 - Administration.

    Science.gov (United States)

    2010-01-01

    ... administration of the Program in the State in accordance with the provisions of this part, 7 CFR parts 235, 245, 15, 15a, 15b and, as applicable, 7 CFR part 3015, 7 CFR part 3016 and 7 CFR part 3019, and with FNS... 7 Agriculture 4 2010-01-01 2010-01-01 false Administration. 215.3 Section 215.3...

  7. 76 FR 66620 - Airworthiness Directives; Bombardier, Inc. Model CL-215-1A10, CL-215-6B11 (CL-215T Variant), and...

    Science.gov (United States)

    2011-10-27

    ... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or.... Model CL-215-1A10, CL- 215-6B11 (CL-215T Variant), and CL-215-6B11 (CL-415 Variant) Airplanes AGENCY... comments. SUMMARY: We are adopting a new airworthiness directive (AD) for certain Bombardier, Inc. Model...

  8. 49 CFR 215.101 - Scope.

    Science.gov (United States)

    2010-10-01

    ... TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components § 215.101 Scope. This subpart contains safety requirements prohibiting a railroad from placing or continuing in service a freight car that has certain defective components. Suspension System...

  9. 32 CFR 552.215 - Responsibilities.

    Science.gov (United States)

    2010-07-01

    ... Demonstrations on the Installation of Aberdeen Proving Ground, Maryland § 552.215 Responsibilities. (a) Director, Law Enforcement and Security, U.S. Army Garrison, Aberdeen Proving Ground, will furnish police...

  10. 49 CFR 215.303 - Stenciling of restricted cars.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Stenciling of restricted cars. 215.303 Section 215... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Stenciling § 215.303 Stenciling of restricted cars. (a) Each restricted railroad freight car that is described in § 215.205(a)...

  11. AcEST: CL215Contig1 [AcEST

    Lifescience Database Archive (English)

    Full Text Available CL215Contig1 2114 10 Adiantum capillus-veneris contig: CL215contig1 sequence. Link to clone list Show CL2...15Contig1 Contig ID CL215Contig1 Length 2114 Number of clones 10 Definition Adiantum c...apillus-veneris contig: CL215contig1 sequence. Link to clone list Link to clone list Clone ID DK951912 DK946

  12. 49 CFR 215.105 - Defective axle.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.105 Defective axle. A railroad may not place or continue in service a car, if— (a) An axle on the car has a crack or is broken; (b) An axle on the car has a gouge in the surface that is— (1)...

  13. 49 CFR 215.103 - Defective wheel.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.103 Defective wheel. A railroad may not place or continue in service a car, if— (a) A wheel flange on the car is worn to a thickness of 7/8 of an inch, or less, at a point 3/8 of an inch above...

  14. 9 CFR 93.215 - Special provisions.

    Science.gov (United States)

    2010-01-01

    ....215 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... veterinary inspector at the United States port of entry that the poultry are the identical poultry covered by... the Royal Agricultural Winter Fair at Toronto or other publicly recognized expositions in...

  15. 49 CFR 212.215 - Locomotive inspector.

    Science.gov (United States)

    2010-10-01

    ... applicable sections of the Safety Glazing Standards (49 CFR part 223), Locomotive Safety Standards (49 CFR part 229), Safety Appliance Standards (49 CFR part 231) and Power Brake Standards (49 CFR part 232), to... 49 Transportation 4 2010-10-01 2010-10-01 false Locomotive inspector. 212.215 Section...

  16. 23 CFR 645.215 - Approvals.

    Science.gov (United States)

    2010-04-01

    .... 109, 111, and 116, and 23 CFR 1.23 and 1.27, and meet the requirements of this regulation, the FHWA... Accommodation of Utilities § 645.215 Approvals. (a) Each State transportation department shall submit a... the FHWA for prior concurrence, except when the proposed installation is not in accordance with...

  17. 45 CFR 150.215 - Extension for good cause.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Extension for good cause. 150.215 Section 150.215... Are Failing To Substantially Enforce HIPAA Requirements § 150.215 Extension for good cause. CMS may extend, for good cause, the time the State has for responding to the notice described in § 150.213...

  18. 41 CFR 101-6.215-3 - Supervision and coordination.

    Science.gov (United States)

    2010-07-01

    ... coordination. 101-6.215-3 Section 101-6.215-3 Public Contracts and Property Management Federal Property...-Nondiscrimination in Programs Receiving Federal Financial Assistance § 101-6.215-3 Supervision and coordination. The... provided in § 101-6.213), including the achievement of effective coordination and maximum uniformity...

  19. 40 CFR 180.215 - Naled; tolerances for residues.

    Science.gov (United States)

    2010-07-01

    ....215 Section 180.215 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific Tolerances § 180.215... insecticide naled (1,2-dibromo-2,2-dichloro-ethyl dimethyl phosphate) and its conversion product...

  20. 25 CFR 215.7 - Advertisement of sale of leases.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Advertisement of sale of leases. 215.7 Section 215.7 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEAD AND ZINC MINING OPERATIONS AND LEASES, QUAPAW AGENCY § 215.7 Advertisement of sale of leases. Upon authority being granted...

  1. 25 CFR 215.24 - Books and accounts.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Books and accounts. 215.24 Section 215.24 Indians BUREAU... LEASES, QUAPAW AGENCY § 215.24 Books and accounts. (a) The lessee shall maintain books in which shall be... land. (b) An audit of the lessee's accounts and books shall be made semiannually, or at such...

  2. 49 CFR 1542.215 - Law enforcement support.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Law enforcement support. 1542.215 Section 1542.215..., DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY AIRPORT SECURITY Operations § 1542.215 Law... program under § 1542.103(a) or (b) must provide: (1) Law enforcement personnel in the number and...

  3. 48 CFR 215.407-3 - Forward pricing rate agreements.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Forward pricing rate agreements. 215.407-3 Section 215.407-3 Federal Acquisition Regulations System DEFENSE ACQUISITION... Contract Pricing 215.407-3 Forward pricing rate agreements. (b)(i) Use forward pricing rate agreement...

  4. 40 CFR 21.5 - Issuance of statements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Issuance of statements. 21.5 Section 21.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL SMALL BUSINESS § 21.5 Issuance of statements. (a) Upon application by a small business concern pursuant to § 21.3 the...

  5. 20 CFR 655.215 - Territory of Guam.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Territory of Guam. 655.215 Section 655.215 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR TEMPORARY EMPLOYMENT OF FOREIGN WORKERS IN THE UNITED STATES Labor Certification Process for Logging Employment and Non-H-2A Agricultural Employment § 655.215 Territory...

  6. 29 CFR 2.15 - Protection of witnesses.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Protection of witnesses. 2.15 Section 2.15 Labor Office of the Secretary of Labor GENERAL REGULATIONS Audiovisual Coverage of Administrative Hearings § 2.15 Protection of witnesses. A witness has the right, prior to or during his testimony, to exclude...

  7. 12 CFR 215.3 - Extension of credit.

    Science.gov (United States)

    2010-01-01

    ... participating bank, not by the originating bank. (f) Tangible economic benefit rule—(1) In general. An extension... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Extension of credit. 215.3 Section 215.3 Banks... OFFICERS, DIRECTORS, AND PRINCIPAL SHAREHOLDERS OF MEMBER BANKS (REGULATION O) § 215.3 Extension of...

  8. 21 CFR 172.215 - Coumarone-indene resin.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Coumarone-indene resin. 172.215 Section 172.215... CONSUMPTION Coatings, Films and Related Substances § 172.215 Coumarone-indene resin. The food additive coumarone-indene resin may be safely used on grapefruit, lemons, limes, oranges, tangelos, and tangerines...

  9. 40 CFR 5.215 - Membership practices of certain organizations.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Membership practices of certain organizations. 5.215 Section 5.215 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL... Coverage § 5.215 Membership practices of certain organizations. (a) Social fraternities and...

  10. 23 CFR 668.215 - Programming and project procedures.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215...

  11. 10 CFR 1042.215 - Membership practices of certain organizations.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Membership practices of certain organizations. 1042.215 Section 1042.215 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 1042.215...

  12. 10 CFR 5.215 - Membership practices of certain organizations.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Membership practices of certain organizations. 5.215 Section 5.215 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 5.215 Membership practices...

  13. 21 CFR 1311.215 - Internal audit trail.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Internal audit trail. 1311.215 Section 1311.215... ORDERS AND PRESCRIPTIONS (Eff. 6-1-10) Electronic Prescriptions § 1311.215 Internal audit trail. (a) The... with audit trail functions. (6) For application service providers, attempted or successful...

  14. 31 CFR 0.215 - Possession of weapons and explosives.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Possession of weapons and explosives. 0.215 Section 0.215 Money and Finance: Treasury Office of the Secretary of the Treasury DEPARTMENT OF THE TREASURY EMPLOYEE RULES OF CONDUCT Rules of Conduct § 0.215 Possession of weapons...

  15. 36 CFR 21.5 - Therapeutic bathing requirements.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Therapeutic bathing requirements. 21.5 Section 21.5 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR HOT SPRINGS NATIONAL PARK; BATHHOUSE REGULATIONS § 21.5 Therapeutic bathing requirements....

  16. 29 CFR 1910.215 - Abrasive wheel machinery.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 5 2010-07-01 2010-07-01 false Abrasive wheel machinery. 1910.215 Section 1910.215 Labor... OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.215 Abrasive wheel machinery. (a... wheel in motion. (5) Excluded machinery. Natural sandstone wheels and metal, wooden, cloth, or...

  17. Groundwater seepage from the Ranger uranium mine tailings dam: radioisotopes of radium, thorium and actinium. Supervising Scientist report 106

    International Nuclear Information System (INIS)

    Monitoring of bores near the Ranger uranium mine tailings dam has revealed deterioration in water quality in several bores since 1983. In a group of bores to the north of the dam, increases have been observed of up to 500 times for sulphate concentrations and of up to 5 times for 226Ra concentrations. Results are presented here of measurements of members of the uranium, thorium and actinium decay series in borewater samples collected between 1985 and 1993. In particular, measurements of all four naturally-occurring radium isotopes have been used in an investigation of the mechanism of radium concentration changes. For the most seepage-affected bores the major findings of the study include: 228Ra/226Ra 223Ra /226Ra and 224Ra/228Ra ratios all increased over the course of the study; barium concentrations show high seasonal variability, being lower in November than May, but strontium concentrations show a steady increase with time. Calculations show that the groundwater is probably saturated with respect to barite but not with respect to celestite or anglesite; sulphide concentrations are low in comparison with sulphate, and are higher in November than in May; and 227Ac concentrations have increased with time, but do not account for the high 223Ra/226Ra ratios. It is concluded on the basis of these observations that increases in Ra isotope concentrations observed in a number of seepage-affected bores arise from increases in salinity leading to desorption of radium from adsorption sites in the vicinity of the bore rather by direct transport of radium from the tailings. Increased salinity is also causing the observed increases in 227Ac and strontium concentrations, while formation of a barite solid phase in the groundwater is causing the removal of some radium from solution. This is the cause of the increasing radium isotope ratios noted above

  18. 48 CFR 2515.215-70 - NSF negotiation authorities.

    Science.gov (United States)

    2010-10-01

    ... CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Negotiation Authorities 2515.215-70 NSF... entered into through negotiation rather than formal advertising. The Foundation's contracting officer...

  19. 37 CFR 1.215 - Patent application publication.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Patent application publication. 1.215 Section 1.215 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES National Processing Provisions...

  20. 32 CFR 215.8 - Organization and administration.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Organization and administration. 215.8 Section...) MISCELLANEOUS EMPLOYMENT OF MILITARY RESOURCES IN THE EVENT OF CIVIL DISTURBANCES § 215.8 Organization and administration. (a) A DoD Civil Disturbance Steering Committee will be established to provide advice...

  1. 48 CFR 452.215-72 - Amendments to Proposals.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Amendments to Proposals. 452.215-72 Section 452.215-72 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE CLAUSES... vertical lines adjacent to the change. The offeror shall include the date of the amendment on the...

  2. 48 CFR 1652.215-71 - Investment Income.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Investment Income. 1652.215... 1652.215-71 Investment Income. As prescribed in 1615.470-1, the following clause shall be inserted in all FEHBP contracts based on cost analysis: Investment Income (JAN 1998) (a) The Carrier shall...

  3. 20 CFR 416.215 - You leave the United States.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false You leave the United States. 416.215 Section... Eligible § 416.215 You leave the United States. You lose your eligibility for SSI benefits for any month during all of which you are outside of the United States. If you are outside of the United States for...

  4. 49 CFR 215.119 - Defective freight car truck.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.119 Defective freight car truck. A railroad may not place or continue in service a... 49 Transportation 4 2010-10-01 2010-10-01 false Defective freight car truck. 215.119 Section...

  5. 2 CFR 215.42 - Codes of conduct.

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Codes of conduct. 215.42 Section 215.42... Codes of conduct. The recipient shall maintain written standards of conduct governing the performance of... interest is not substantial or the gift is an unsolicited item of nominal value. The standards of...

  6. 32 CFR Appendix C to Part 45 - DD Form 215

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 215 C Appendix C to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. C Appendix C to Part 45—DD Form 215 EC21OC91.050...

  7. 42 CFR 57.215 - Records, reports, inspection, and audit.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Records, reports, inspection, and audit. 57.215... FOR CONSTRUCTION OF TEACHING FACILITIES, EDUCATIONAL IMPROVEMENTS, SCHOLARSHIPS AND STUDENT LOANS Health Professions Student Loans § 57.215 Records, reports, inspection, and audit. (a) Each...

  8. 2 CFR 215.45 - Cost and price analysis.

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Cost and price analysis. 215.45 Section 215... ADMINISTRATIVE REQUIREMENTS FOR GRANTS AND AGREEMENTS WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER... Cost and price analysis. Some form of cost or price analysis shall be made and documented in...

  9. 47 CFR 36.215 - Miscellaneous revenue-Account 5200.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Miscellaneous revenue-Account 5200. 36.215..., REVENUES, EXPENSES, TAXES AND RESERVES FOR TELECOMMUNICATIONS COMPANIES 1 Operating Revenues and Certain Income Accounts Operating Revenues § 36.215 Miscellaneous revenue—Account 5200. (a) Directory...

  10. 44 CFR 19.215 - Membership practices of certain organizations.

    Science.gov (United States)

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Membership practices of certain organizations. 19.215 Section 19.215 Emergency Management and Assistance FEDERAL EMERGENCY... Title IX regulations do not apply to the membership practices of a voluntary youth service...

  11. 47 CFR 97.215 - Telecommand of model craft.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Telecommand of model craft. 97.215 Section 97... AMATEUR RADIO SERVICE Special Operations § 97.215 Telecommand of model craft. An amateur station transmitting signals to control a model craft may be operated as follows: (a) The station...

  12. 48 CFR 215.470 - Estimated data prices.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Estimated data prices. 215.470 Section 215.470 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM....470 Estimated data prices. (a) DoD requires estimates of the prices of data in order to evaluate...

  13. 14 CFR 21.5 - Airplane or Rotorcraft Flight Manual.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Airplane or Rotorcraft Flight Manual. 21.5... CERTIFICATION PROCEDURES FOR PRODUCTS AND PARTS General § 21.5 Airplane or Rotorcraft Flight Manual. (a) With each airplane or rotorcraft that was not type certificated with an Airplane or Rotorcraft Flight...

  14. 48 CFR 333.215-70 - Contract clauses.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Contract clauses. 333.215... CONTRACTING REQUIREMENTS PROTESTS, DISPUTES, AND APPEALS Disputes and Appeals 333.215-70 Contract clauses. (a) The Contracting Officer shall insert the clause in 352.233-70, Choice of Law (Overseas),...

  15. 48 CFR 215.407-5 - Estimating systems.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Estimating systems. 215.407-5 Section 215.407-5 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  16. 45 CFR 618.215 - Membership practices of certain organizations.

    Science.gov (United States)

    2010-10-01

    ....215 Section 618.215 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE... institutions of higher education. (b) YMCA, YWCA, Girl Scouts, Boy Scouts, and Camp Fire Girls. These Title IX... Young Women's Christian Association (YWCA), the Girl Scouts, the Boy Scouts, and Camp Fire Girls....

  17. 34 CFR 668.215 - Average rates appeals.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 3 2010-07-01 2010-07-01 false Average rates appeals. 668.215 Section 668.215... Average rates appeals. (a) Eligibility. (1) You may appeal a notice of a loss of eligibility under § 668... as an average rate under § 668.202(d)(2). (2) You may appeal a notice of a loss of eligibility...

  18. 30 CFR 77.215-3 - Refuse piles: certification.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles: certification. 77.215-3 Section... COAL MINES Surface Installations § 77.215-3 Refuse piles: certification. (a) Within 180 days following written notification by the District Manager that a refuse pile can present a hazard, the person...

  19. 30 CFR 77.215-1 - Refuse piles; identification.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles; identification. 77.215-1 Section... COAL MINES Surface Installations § 77.215-1 Refuse piles; identification. A permanent identification marker, at least six feet high and showing the refuse pile identification number as assigned by...

  20. 30 CFR 77.215-2 - Refuse piles; reporting requirements.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles; reporting requirements. 77.215-2... COAL MINES Surface Installations § 77.215-2 Refuse piles; reporting requirements. (a) The proposed location of a new refuse pile shall be reported to and acknowledged in writing by the District...

  1. 30 CFR 77.215 - Refuse piles; construction requirements.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles; construction requirements. 77.215... COAL MINES Surface Installations § 77.215 Refuse piles; construction requirements. (a) Refuse deposited on a pile shall be spread in layers and compacted in such a manner so as to minimize the flow of...

  2. 30 CFR 77.215-4 - Refuse piles; abandonment.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles; abandonment. 77.215-4 Section 77... MINES Surface Installations § 77.215-4 Refuse piles; abandonment. When a refuse pile is to be abandoned... refuse pile shall be abandoned in accordance with a plan submitted by the operator and approved by...

  3. 49 CFR 215.111 - Defective plain bearing.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.111 Defective plain bearing. A railroad may not place or continue in service a car, if the car...

  4. 40 CFR 98.215 - Procedures for estimating missing data.

    Science.gov (United States)

    2010-07-01

    ... PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Miscellaneous Uses of Carbonate § 98.215 Procedures... emissions calculations is required. Therefore, whenever a quality-assured value of a required parameter...

  5. 29 CFR 1603.215 - Record of hearings.

    Science.gov (United States)

    2010-07-01

    ... EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE GOVERNMENT EMPLOYEE RIGHTS ACT OF 1991 Hearings § 1603.215 Record of hearings. (a) All...

  6. 48 CFR 215.404-76 - Reporting profit and fee statistics.

    Science.gov (United States)

    2010-10-01

    ... statistics. 215.404-76 Section 215.404-76 Federal Acquisition Regulations System DEFENSE ACQUISITION... Contract Pricing 215.404-76 Reporting profit and fee statistics. Follow the procedures at PGI 215.404-76 for reporting profit and fee statistics....

  7. 7 CFR 1260.215 - Patents, copyrights, inventions and publications.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Patents, copyrights, inventions and publications... AGRICULTURE BEEF PROMOTION AND RESEARCH Beef Promotion and Research Order Miscellaneous § 1260.215 Patents, copyrights, inventions and publications. (a) Any patents, copyrights, inventions or publications...

  8. 18 CFR 157.215 - Underground storage testing and development.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Underground storage... of the Natural Gas Act for Certain Transactions and Abandonment § 157.215 Underground storage testing... observation wells for the testing or development of underground reservoirs for the possible storage of gas,...

  9. 48 CFR 215.404-71-1 - General.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 215... range provides values based on above normal or below normal conditions. In the price negotiation... risk; (2) Contract type risk; (3) Facilities capital employed; and (4) Cost efficiency. (b)...

  10. 2 CFR 215.21 - Standards for financial management systems.

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Standards for financial management systems... Financial and Program Management § 215.21 Standards for financial management systems. (a) Federal awarding... information whenever practical. (b) Recipients' financial management systems shall provide for the...

  11. 49 CFR 215.113 - Defective plain bearing wedge.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.113 Defective plain bearing wedge. A railroad may not place or continue in service a car, if a plain bearing wedge on that car is— (a) Missing; (b) Cracked; (c) Broken; or (d) Not...

  12. 49 CFR 215.117 - Defective roller bearing adapter.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.117 Defective roller bearing adapter. A railroad may not place or continue in service a car, if the car has a roller bearing adapter that is— (a) Cracked or broken; (b) Not in its...

  13. 49 CFR 215.115 - Defective roller bearing.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.115 Defective roller bearing. (a) A railroad may not place or continue in service a car, if the car has— (1) A roller bearing that shows signs of having been overheated as evidenced by—...

  14. 49 CFR 215.107 - Defective plain bearing box: General.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Freight Car Components Suspension System § 215.107 Defective plain bearing box: General. A railroad may not place or continue in service a car, if the car has— (a) A plain bearing box that does not contain visible free oil; (b) A...

  15. 20 CFR 628.215 - State Human Resource Investment Council.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false State Human Resource Investment Council. 628... PROGRAMS UNDER TITLE II OF THE JOB TRAINING PARTNERSHIP ACT State Planning § 628.215 State Human Resource..., 702, and 703 of the Act, establish a State Human Resource Investment Council (HRIC). The...

  16. 48 CFR 215.404-71-5 - Cost efficiency factor.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Cost efficiency factor... Contract Pricing 215.404-71-5 Cost efficiency factor. (a) This special factor provides an incentive for... these efforts (Block 29). (b) To determine if using this factor is appropriate, the contracting...

  17. 48 CFR 215.370-2 - Evaluation factor.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Evaluation factor. 215.370....370-2 Evaluation factor. In accordance with Section 819 of the National Defense Authorization Act for Fiscal Year 2006 (Pub. L. 109-163), the contracting officer may use an evaluation factor that...

  18. 36 CFR 215.5 - Legal notice of proposed actions.

    Science.gov (United States)

    2010-07-01

    ... the notice of availability (NOA) in the Federal Register (§ 215.6(a)(2)). The legal notice must be published after the NOA and contain the NOA publication date. (vi) A statement that only those who submit... proposed action to be analyzed and documented in an EA. The publication date of the NOA in the...

  19. 36 CFR 215.19 - Appeal Reviewing Officer.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Appeal Reviewing Officer. 215... Reviewing Officer. (a) Designation. The Appeal Reviewing Officer shall be: (1) Designated by the Chief or... Appeal Reviewing Officer shall review an appeal and the decision documentation and make a...

  20. 6 CFR 27.215 - Security vulnerability assessments.

    Science.gov (United States)

    2010-01-01

    ...-risk, the facility must complete a Security Vulnerability Assessment. A Security Vulnerability... 6 Domestic Security 1 2010-01-01 2010-01-01 false Security vulnerability assessments. 27.215... provided in § 27.235, a covered facility must complete the Security Vulnerability Assessment through...

  1. 20 CFR 725.215 - Determination of dependency; surviving spouse.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Determination of dependency; surviving spouse...) § 725.215 Determination of dependency; surviving spouse. An individual who is the miner's surviving... service serving on active duty (as defined in § 404.1019 of this title), and the surviving spouse...

  2. 2 CFR 215.15 - Metric system of measurement.

    Science.gov (United States)

    2010-01-01

    ... Programs” (56 FR 35801, 3 CFR, 1991 Comp., p. 343). ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Metric system of measurement. 215.15 Section... measurement. The Metric Conversion Act, as amended by the Omnibus Trade and Competitiveness Act (15 U.S.C....

  3. 78 FR 65453 - Hazardous Materials: Corrections and Response to Administrative Appeals (HM-215K, HM-215L, HM...

    Science.gov (United States)

    2013-10-31

    ... other solid propellants that are used as powder for small arms and have been classed as Division 1.3, 1...: As with Special Provision 16, by changing the terminology in the final rule from ``solid propellants... FR 3308, HM-215K): In addition, packages containing such articles offered for transportation...

  4. 50 CFR 226.215 - Critical habitat for the North Pacific Right Whale (Eubalaena japonica).

    Science.gov (United States)

    2010-10-01

    ... Right Whale (Eubalaena japonica). 226.215 Section 226.215 Wildlife and Fisheries NATIONAL MARINE... DESIGNATED CRITICAL HABITAT § 226.215 Critical habitat for the North Pacific Right Whale (Eubalaena japonica). (a) Primary Constituent Elements. The primary constituent elements of the North Pacific right...

  5. 12 CFR 215.5 - Additional restrictions on loans to executive officers of member banks.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Additional restrictions on loans to executive officers of member banks. 215.5 Section 215.5 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS... MEMBER BANKS (REGULATION O) § 215.5 Additional restrictions on loans to executive officers of...

  6. 33 CFR 162.215 - Lake Tahoe, Nev.; restricted area adjacent to Nevada Beach.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Lake Tahoe, Nev.; restricted area adjacent to Nevada Beach. 162.215 Section 162.215 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 162.215 Lake Tahoe, Nev.; restricted area adjacent to Nevada Beach. (a) The restricted area....

  7. 40 CFR 1065.215 - Pressure transducers, temperature sensors, and dewpoint sensors.

    Science.gov (United States)

    2010-07-01

    ... sensors, such as a wet-bulb/dry-bulb psychrometer, where appropriate. Flow-Related Measurements ... sensors, and dewpoint sensors. 1065.215 Section 1065.215 Protection of Environment ENVIRONMENTAL... Measurement of Engine Parameters and Ambient Conditions § 1065.215 Pressure transducers, temperature...

  8. 48 CFR 52.215-17 - Waiver of Facilities Capital Cost of Money.

    Science.gov (United States)

    2010-10-01

    ... Capital Cost of Money. 52.215-17 Section 52.215-17 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.215-17 Waiver of Facilities Capital Cost of Money. As prescribed in 15.408(i), insert the following clause: Waiver of Facilities Capital Cost of Money (OCT 1997) The Contractor did...

  9. 48 CFR 52.215-16 - Facilities Capital Cost of Money.

    Science.gov (United States)

    2010-10-01

    ... Money. 52.215-16 Section 52.215-16 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION....215-16 Facilities Capital Cost of Money. As prescribed in 15.408(h), insert the following provision: Facilities Capital Cost of Money (JUN 2003) (a) Facilities capital cost of money will be an allowable...

  10. XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation

    DEFF Research Database (Denmark)

    Mortuza, Gulnahar B; Cavazza, Tommaso; Garcia-Mayoral, Maria Flor;

    2014-01-01

    215 (chTOG), dissecting the mechanism by which their interaction promotes microtubule elongation during spindle assembly. Using SAXS, we show that the TACC domain (TD) is an elongated structure that mediates the interaction with the C terminus of XMAP215. Our data suggest that one TD and two XMAP215...... molecules associate to form a four-helix coiled-coil complex. A hybrid methods approach was used to define the precise regions of the TACC heptad repeat and the XMAP215 C terminus required for assembly and functioning of the complex. We show that XTACC3 can induce the recruitment of larger amounts of XMAP...

  11. Critical review of 215 nonpalpable breast lesions; Revisione critica di 215 casi di lesioni non palpabili della mammella

    Energy Technology Data Exchange (ETDEWEB)

    Pastore, G.; Marano, P.; Romani, M.; Costantini, M.; Belli, P. [Rome Policlinico universitario Gemelli, Rome (Italy). Ist. di radiologia

    1999-05-01

    From 1988 to October 1998, 215 women with nonpalpable breast lesions suspected at mammography were examined. The lesions were removed after stereotaxic or US (ultrasound) location and a radiograph of the surgical specimen was always performed. Mammographic patterns were interpreted retrospectively by two blinded radiologist experienced in breast lesions and were classified as poorly-highly suspicious calcifications and parenchymal distortions. Radiographic findings were compared with surgical results and the data used to calculate the B/M, positive predictive value (PPV) for malignancy and the trend of operator's performance. Modern techniques permit to detect a very high number of in situ breast calcifications. [Italian] Dal 1988 all'ottobre 1998 sono state studiate 215 donne con lesioni non palpabili della mammella, con eta' compresa tra 26 e 78 anni. Le lesioni sono state sospettate all'esame mammografico, asportate previa localizzazione stereotassica o ecografica e sottoposte ad esame istologico. E' stata eseguita sempre una radiografia del pezzo operatorio. I reperti radiografici sono stati confrontati con quelli istologici e l'elaborazione dei dati ha mirato a calcolare il rapporto tra lesioni benigne e maligne, il valore predittivo positivo per la malignita' del segno radiologico e l'andamento della qualita' di competenza degli operatori nel corso degli ultimi dieci anni. La revisione della casistica conferma che le moderne apparecchiature consentono di individuare un numero consistentemente elevato di carcinomi in situ (23%).

  12. 31 CFR 20.215 - What must I include in my drug-free awareness program?

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false What must I include in my drug-free awareness program? 20.215 Section 20.215 Money and Finance: Treasury Office of the Secretary of the Treasury GOVERNMENTWIDE REQUIREMENTS FOR DRUG-FREE WORKPLACE (FINANCIAL ASSISTANCE) Requirements for Recipients Other...

  13. 20 CFR 664.215 - Must youth participants be registered to participate in the youth program?

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Must youth participants be registered to participate in the youth program? 664.215 Section 664.215 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR YOUTH ACTIVITIES UNDER TITLE I OF THE WORKFORCE INVESTMENT ACT...

  14. 48 CFR 52.215-15 - Pension adjustments and asset reversions.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Pension adjustments and asset reversions. 52.215-15 Section 52.215-15 Federal Acquisition Regulations System FEDERAL ACQUISITION... the numerator of the fraction at 48 CFR 9904.413-50(c)(12)(vi) shall be the sum of the pension...

  15. 9 CFR 113.215 - Bovine Virus Diarrhea Vaccine, Killed Virus.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bovine Virus Diarrhea Vaccine, Killed Virus. 113.215 Section 113.215 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS...

  16. 21 CFR 1404.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Which nonprocurement transactions are not covered transactions? 1404.215 Section 1404.215 Food and Drugs OFFICE OF NATIONAL DRUG CONTROL POLICY GOVERNMENTWIDE... transaction that the Office of National Drug Control Policy needs to respond to a national or...

  17. 48 CFR 253.215-70 - DD Form 1547, Record of Weighted Guidelines Application.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1547, Record of... Forms 253.215-70 DD Form 1547, Record of Weighted Guidelines Application. Follow the procedures at PGI 253.215-70 for completing DD Form 1547....

  18. 48 CFR 215.404-70 - DD Form 1547, Record of Weighted Guidelines Method Application.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1547, Record of... TYPES CONTRACTING BY NEGOTIATION Contract Pricing 215.404-70 DD Form 1547, Record of Weighted Guidelines Method Application. Follow the procedures at PGI 215.404-70 for use of DD Form 1547 whenever a...

  19. 49 CFR Appendix C to Part 215 - FRA Freight Car Standards Defect Code

    Science.gov (United States)

    2010-10-01

    ... description of defects on bad order tags affixed to cars being moved for repair under § 215.9. However, it may...) Not located in design position. 215.115Defective roller bearing. (A)(1) Overheated; (2) Loose or... roller bearing adapter. (A) Cracked or broken. (B) Not in design position. (C) Worn excessively as...

  20. 49 CFR 215.9 - Movement of defective cars for repair.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Movement of defective cars for repair. 215.9... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS General § 215.9 Movement of defective cars for repair. (a) A railroad freight car which has any component described as defective in...

  1. 25 CFR 215.21 - Payment of gross production tax on lead and zinc.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Payment of gross production tax on lead and zinc. 215.21... ZINC MINING OPERATIONS AND LEASES, QUAPAW AGENCY § 215.21 Payment of gross production tax on lead and zinc. The superintendent of the Quapaw Indian Agency is hereby authorized and directed to pay at...

  2. 49 CFR Appendix A to Part 215 - Railroad Freight Car Components

    Science.gov (United States)

    2010-10-01

    ... Part 215—Railroad Freight Car Components List of components whose use is restricted by § 215.203 of... with letters “RJ” stamped on the end of the journal. C.Couplers: 1. AAR type “D”, top or bottom.... 2. Farlow draft attachment. E.Plain journal bearings: Cartridge type. F.Roller bearings: 1....

  3. 29 CFR 1471.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-07-01

    ... transactions? 1471.215 Section 1471.215 Labor Regulations Relating to Labor (Continued) FEDERAL MEDIATION AND... foreign governments or foreign governmental entities. (b) A benefit to an individual as a personal.... (c) Federal employment. (d) A transaction that the Federal Mediation and Conciliation Service...

  4. 24 CFR 598.215 - What are the purpose and content of the strategic plan?

    Science.gov (United States)

    2010-04-01

    ... the strategic plan? 598.215 Section 598.215 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND... strategic plan? (a) Principles of strategic plan. The strategic plan, which accompanies the application...

  5. 48 CFR 215.404-72 - Modified weighted guidelines method for nonprofit organizations other than FFRDCs.

    Science.gov (United States)

    2010-10-01

    ... guidelines method for nonprofit organizations other than FFRDCs. 215.404-72 Section 215.404-72 Federal... guidelines method for nonprofit organizations other than FFRDCs. (a) Definition. As used in this subpart, a nonprofit organization is a business entity— (1) That operates exclusively for charitable, scientific,...

  6. 12 CFR 215.7 - Extensions of credit outstanding on March 10, 1979.

    Science.gov (United States)

    2010-01-01

    ... the case of a State member bank, and explain the reasons why all the extensions of credit cannot be... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Extensions of credit outstanding on March 10, 1979. 215.7 Section 215.7 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE...

  7. 41 CFR 102-85.215 - What if another customer agency forces a GSA customer to move?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What if another customer agency forces a GSA customer to move? 102-85.215 Section 102-85.215 Public Contracts and Property... § 102-85.215 What if another customer agency forces a GSA customer to move? If a GSA customer agency,...

  8. 12 CFR 215.9 - Disclosure of credit from member banks to executive officers and principal shareholders.

    Science.gov (United States)

    2010-01-01

    ... executive officers and principal shareholders. 215.9 Section 215.9 Banks and Banking FEDERAL RESERVE SYSTEM... SHAREHOLDERS OF MEMBER BANKS (REGULATION O) § 215.9 Disclosure of credit from member banks to executive officers and principal shareholders. (a) Definitions. For the purposes of this section, the...

  9. 49 CFR 40.215 - What information about the DER do employers have to provide to BATs and STTs?

    Science.gov (United States)

    2010-10-01

    ... to provide to BATs and STTs? 40.215 Section 40.215 Transportation Office of the Secretary of... Personnel § 40.215 What information about the DER do employers have to provide to BATs and STTs? As an employer, you must provide to the STTs and BATs the name and telephone number of the appropriate DER (and...

  10. The CDX1–microRNA-215 axis regulates colorectal cancer stem cell differentiation

    Science.gov (United States)

    Jones, Matthew F.; Hara, Toshifumi; Francis, Princy; Li, Xiao Ling; Bilke, Sven; Zhu, Yuelin; Pineda, Marbin; Subramanian, Murugan; Bodmer, Walter F.; Lal, Ashish

    2015-01-01

    The transcription factor caudal-type homeobox 1 (CDX1) is a key regulator of differentiation in the normal colon and in colorectal cancer (CRC). CDX1 activates the expression of enterocyte genes, but it is not clear how the concomitant silencing of stem cell genes is achieved. MicroRNAs (miRNAs) are important mediators of gene repression and have been implicated in tumor suppression and carcinogenesis, but the roles of miRNAs in differentiation, particularly in CRC, remain poorly understood. Here, we identified microRNA-215 (miR-215) as a direct transcriptional target of CDX1 by using high-throughput small RNA sequencing to profile miRNA expression in two pairs of CRC cell lines: CDX1-low HCT116 and HCT116 with stable CDX1 overexpression, and CDX1-high LS174T and LS174T with stable CDX1 knockdown. Validation of candidate miRNAs identified by RNA-seq in a larger cell-line panel revealed miR-215 to be most significantly correlated with CDX1 expression. Quantitative ChIP–PCR and promoter luciferase assays confirmed that CDX1 directly activates miR-215 transcription. miR-215 expression is depleted in FACS-enriched cancer stem cells compared with unsorted samples. Overexpression of miR-215 in poorly differentiated cell lines causes a decrease in clonogenicity, whereas miR-215 knockdown increases clonogenicity and impairs differentiation in CDX1-high cell lines. We identified the genome-wide targets of miR-215 and found that miR-215 mediates the repression of cell cycle and stemness genes downstream of CDX1. In particular, the miR-215 target gene BMI1 has been shown to promote stemness and self-renewal and to vary inversely with CDX1. Our work situates miR-215 as a link between CDX1 expression and BMI1 repression that governs differentiation in CRC. PMID:25775580

  11. Importance of the CEP215-Pericentrin Interaction for Centrosome Maturation during Mitosis

    OpenAIRE

    Seongjae Kim; Kunsoo Rhee

    2014-01-01

    At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The depletion phenotypes indicate that CEP215 is essential for centrosome maturation and bipolar spindle formation. Here, we performed a series of knockdown-rescue experiments to link the protein-prot...

  12. A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer.

    Science.gov (United States)

    Chavali, Pavithra L; Chandrasekaran, Gayathri; Barr, Alexis R; Tátrai, Péter; Taylor, Chris; Papachristou, Evaggelia K; Woods, C Geoffrey; Chavali, Sreenivas; Gergely, Fanni

    2016-01-01

    Numerical centrosome aberrations underlie certain developmental abnormalities and may promote cancer. A cell maintains normal centrosome numbers by coupling centrosome duplication with segregation, which is achieved through sustained association of each centrosome with a mitotic spindle pole. Although the microcephaly- and primordial dwarfism-linked centrosomal protein CEP215 has been implicated in this process, the molecular mechanism responsible remains unclear. Here, using proteomic profiling, we identify the minus end-directed microtubule motor protein HSET as a direct binding partner of CEP215. Targeted deletion of the HSET-binding domain of CEP215 in vertebrate cells causes centrosome detachment and results in HSET depletion at centrosomes, a phenotype also observed in CEP215-deficient patient-derived cells. Moreover, in cancer cells with centrosome amplification, the CEP215-HSET complex promotes the clustering of extra centrosomes into pseudo-bipolar spindles, thereby ensuring viable cell division. Therefore, stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could promote survival of cancer cells containing supernumerary centrosomes. PMID:26987684

  13. Molecular mechanism of chemoresistance by miR-215 in osteosarcoma and colon cancer cells

    Directory of Open Access Journals (Sweden)

    Formentini Andrea

    2010-04-01

    Full Text Available Abstract Background Translational control mediated by non-coding microRNAs (miRNAs plays a key role in the mechanism of cellular resistance to anti-cancer drug treatment. Dihydrofolate reductase (DHFR and thymidylate synthase (TYMS, TS are two of the most important targets for antifolate- and fluoropyrimidine-based chemotherapies in the past 50 years. In this study, we investigated the roles of miR-215 in the chemoresistance to DHFR inhibitor methotrexate (MTX and TS inhibitor Tomudex (TDX. Results The protein levels of both DHFR and TS were suppressed by miR-215 without the alteration of the target mRNA transcript levels. Interestingly, despite the down-regulation of DHFR and TS proteins, ectopic expression of miR-215 resulted in a decreased sensitivity to MTX and TDX. Paradoxically, gene-specific small-interfering RNAs (siRNAs against DHFR or TS had the opposite effect, increasing sensitivity to MTX and TDX. Further studies revealed that over-expression of miR-215 inhibited cell proliferation and triggered cell cycle arrest at G2 phase, and that this effect was accompanied by a p53-dependent up-regulation of p21. The inhibitory effect on cell proliferation was more pronounced in cell lines containing wild-type p53, but was not seen in cells transfected with siRNAs against DHFR or TS. Moreover, denticleless protein homolog (DTL, a cell cycle-regulated nuclear and centrosome protein, was confirmed to be one of the critical targets of miR-215, and knock-down of DTL by siRNA resulted in enhanced G2-arrest, p53 and p21 induction, and reduced cell proliferation. Additionally, cells subjected to siRNA against DTL exhibited increased chemoresistance to MTX and TDX. Endogenous miR-215 was elevated about 3-fold in CD133+HI/CD44+HI colon cancer stem cells that exhibit slow proliferating rate and chemoresistance compared to control bulk CD133+/CD44+ colon cancer cells. Conclusions Taken together, our results indicate that miR-215, through the suppression

  14. 22 CFR 215.10 - Disclosure of record to person other than the individual to whom it pertains.

    Science.gov (United States)

    2010-04-01

    ... individual to whom it pertains. 215.10 Section 215.10 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT... individual to whom it pertains. (a) Subject to the conditions of paragraphs (b) and (c) of this section, the... enforcement activity for which it is sought; (8) To a responsible person pursuant to a showing of...

  15. 48 CFR 852.215-70 - Service-disabled veteran-owned and veteran-owned small business evaluation factors.

    Science.gov (United States)

    2010-10-01

    ...-owned and veteran-owned small business evaluation factors. 852.215-70 Section 852.215-70 Federal...-owned small business evaluation factors. As prescribed in 815.304-71(a), insert the following clause: Service-Disabled Veteran-Owned and Veteran-Owned Small Business Evaluation Factors (DEC 2009) (a) In...

  16. 31 CFR 585.215 - Conveyances and cargo suspected of being in violation of United Nations sanctions; detention...

    Science.gov (United States)

    2010-07-01

    ... being in violation of United Nations sanctions; detention; blocking. 585.215 Section 585.215 Money and... and cargo suspected of being in violation of United Nations sanctions; detention; blocking. (a) Except... § 585.201, but which are suspected of a violation of United Nations Security Council Resolutions No....

  17. 48 CFR 52.215-18 - Reversion or Adjustment of Plans for Postretirement Benefits (PRB) Other Than Pensions.

    Science.gov (United States)

    2010-10-01

    ... Plans for Postretirement Benefits (PRB) Other Than Pensions. 52.215-18 Section 52.215-18 Federal... Postretirement Benefits (PRB) Other Than Pensions. As prescribed in 15.408(j), insert the following clause: Reversion or Adjustment of Plans for Postretirement Benefits (PRB) Other Than Pensions (JUL 2005) (a)...

  18. 30 CFR 204.215 - Are the information collection requirements in this subpart approved by the Office of Management...

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Are the information collection requirements in this subpart approved by the Office of Management and Budget (OMB)? 204.215 Section 204.215 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT...

  19. 49 CFR 215.109 - Defective plain bearing box: Journal lubrication system.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Defective plain bearing box: Journal lubrication... Freight Car Components Suspension System § 215.109 Defective plain bearing box: Journal lubrication system...) Metal parts contacting the journal; or (e) Is— (1) Missing; or (2) Not in contact with the journal....

  20. 30 CFR 250.215 - What hydrogen sulfide (H2S) information must accompany the EP?

    Science.gov (United States)

    2010-07-01

    ... methodologies outlined in 40 CFR part 68. ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What hydrogen sulfide (H2S) information must... Contents of Exploration Plans (ep) § 250.215 What hydrogen sulfide (H2S) information must accompany the...

  1. 20 CFR 405.215 - Procedures before a Federal reviewing official.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Procedures before a Federal reviewing... PROCESS FOR ADJUDICATING INITIAL DISABILITY CLAIMS Review of Initial Determinations by a Federal Reviewing Official § 405.215 Procedures before a Federal reviewing official. (a) General. The Federal...

  2. 27 CFR 24.215 - Wine or wine products not for beverage use.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Wine or wine products not... AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS WINE Production of Other Than Standard Wine § 24.215 Wine or wine products not for beverage use. (a) General. Wine, or wine products made from...

  3. 14 CFR 91.215 - ATC transponder and altitude reporting equipment and use.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false ATC transponder and altitude reporting... RULES Equipment, Instrument, and Certificate Requirements § 91.215 ATC transponder and altitude... under part 121 or 135 of this chapter, ATC transponder equipment installed must meet the performance...

  4. 29 CFR 780.215 - Meaning of forestry or lumbering operations.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Meaning of forestry or lumbering operations. 780.215... forestry or lumbering operations. The term forestry or lumbering operations refers to the cultivation and... considers the sec. 13(b)(28) exemption for forestry or logging operations in which not more than...

  5. Molecular and immuno-characteristics of immunoglobulin-like glycoproteins in cancer cell-expressed biomarker, CA215.

    Science.gov (United States)

    Lee, Gregory; Cheung, Anthony P; Li, Bo; Ge, Bixia; Chow, Po-Ming

    2012-01-01

    RP215 monoclonal antibody (Mab) was shown to recognize a specific carbohydrate-associated epitope found in cancer cell-expressed glycoproteins, known as CA215. The membrane-bound and soluble forms of CA215 were detected in almost all of the cancer cells in humans, but rarely found in normal tissues. Through MALDI-TOF MS analysis, it has been reported previously that as much as 40% of the detected tryptic peptides of CA215 showed high degrees of sequence homology to those found in immunoglobulin heavy chains. The cancer cell-derived immunoglobulins were further purified from CA215 by affinity column-linked with goat anti-human IgG for molecular characterizations. Semi-quantitative RT-PCR was used to determine the mRNA levels of various immunoglobulin genes expressed by cancer cells of single or multi-cell origins and compared with those found in normal human serum. The stability of CA215 was investigated under different experimental conditions. It was observed that the RP215-specific epitope in CA215 is stable at neutral pH, in human serum or in mice (half life of 5-18 days), but unstable at extreme pH's (pH ≤ 2.0; pH ≥ 12.0) or high temperatures. Enzyme immunoassays were performed with several secondary antibody probes related to human IgG. It was demonstrated that cancer cell-expressed immunoglobulins with RP215-specific epitope have much lower immunoactivity than that of normal human IgG (≤ 5%), despite the fact that both showed almost identical amino acid sequence in the respective Fc region reported previously. This could be the result of aberrant glycosylation of CA215 in cancer cells. Aberrant glycosylation of glycoproteins may have important biological implications on the proliferation of cancer cells in vitro or in vivo. PMID:22417288

  6. 48 CFR 853.215-70 - VA Form 10-1170, Application for Furnishing Nursing Home Care to Beneficiaries of VA.

    Science.gov (United States)

    2010-10-01

    ..., Application for Furnishing Nursing Home Care to Beneficiaries of VA. 853.215-70 Section 853.215-70 Federal... 853.215-70 VA Form 10-1170, Application for Furnishing Nursing Home Care to Beneficiaries of VA. VA Form 10-1170, Application for Furnishing Nursing Home Care to Beneficiaries of VA, will be used...

  7. Intestinal renal metabolism of L-citrulline and L-arginine following enteral or parenteral infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in mice.

    Science.gov (United States)

    Boelens, Petra G; van Leeuwen, Paul A M; Dejong, Cornelis H C; Deutz, Nicolaas E P

    2005-10-01

    Previously, we observed increased plasma arginine (ARG) concentrations after glutamine (GLN)-enriched diets, in combination with clinical benefits. GLN delivers nitrogen for ARG synthesis, and the present study was designed to quantify the interorgan relationship of exogenous L-GLN or GLN dipeptide, by enteral or parenteral route, contributing to intestinal citrulline (CIT) and renal de novo ARG synthesis in mice. To study this, we used a multicatheterized mouse model with Swiss mice (n = 43) in the postabsorptive state. Stable isotopes were infused into the jugular vein or into the duodenum {per group either free L-[2,(15)N]GLN or dipeptide L-ALA-L-[2,(15)N]GLN, all with L-[ureido-(13)C-(2)H(2)]CIT and L-[guanidino-(15)N(2)-(2)H(2)]ARG} to establish renal and intestinal ARG and CIT metabolism. Blood flow was measured using (14)C-para-aminohippuric acid. Net intestinal CIT release, renal uptake of CIT, and net renal ARG efflux was found, as assessed by arteriovenous flux measurements. Quantitatively, more de novo L-[2,(15)N]CIT was produced when free L-[2,(15)N]GLN was given than when L-ALA-L-[2,(15)N]GLN was given, whereas renal de novo L-[2,(15)N]ARG was similar in all groups. In conclusion, the intestinal-renal axis is hereby proven in mice in that L-[2,(15)N]GLN or dipeptide were both converted into de novo renal L-[2,(15)N]ARG; however, not all was derived from intestinal L-[2,(15)N]CIT production. In this model, the feeding route and form of GLN did not influence de novo renal ARG production derived from GLN.

  8. Downregulation of Plasma miR-215 in Chronic Myeloid Leukemia Patients with Successful Discontinuation of Imatinib

    Directory of Open Access Journals (Sweden)

    Kazuma Ohyashiki

    2016-04-01

    Full Text Available Approximately 40% of chronic myeloid leukemia (CML patients who discontinue imatinib (IM therapy maintain undetectable minimal residual disease (UMRD for more than one year (stopping IM (STOP-IM. To determine a possible biomarker for STOP-IM CML, we examined plasma miRNA expression in CML patients who were able to discontinue IM. We first screened candidate miRNAs in unselected STOP-IM patients, who had sustained UMRD after discontinuing IM for more than six months, in comparison with healthy volunteers, by using a TaqMan low-density array for plasma or exosomes. Exosomal miR-215 and plasma miR-215 were downregulated in the STOP-IM group compared to the control, indicating that the biological relevance of the plasma miR-215 level is equivalent to that of the exosomal level. Next, we performed real-time quantitative RT-PCR in 20 STOP-IM patients, 32 patients with UMRD on continued IM therapy (IM group and 28 healthy volunteers. The plasma miRNA-215 level was significantly downregulated in the STOP-IM group (p < 0.0001; we determined the cut-off level and divided the IM group patients into two groups according to whether the plasma miR-215 was downregulated or not. The IM group patients with a low plasma miR-215 level had a significantly higher total IM intake, compared to the patients with elevated miR-215 levels (p = 0.0229. Functional annotation of miR-215 target genes estimated by the Database for Annotation, Visualization and Integrated Discovery (DAVID bioinformatic tools involved cell cycle, mitosis, DNA repair and cell cycle checkpoint. Our study suggests a possible role of miR-215 in successful IM discontinuation.

  9. 49 CFR Appendix B to Part 215 - Schedule of Civil Penalties 1

    Science.gov (United States)

    2010-10-01

    ... $100,000 for any violation where circumstances warrant. See 49 CFR part 209, appendix A. Failure to... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD FREIGHT CAR SAFETY STANDARDS Pt. 215, App. B Appendix B to... 7,500 (c) Rim thickness of: (1) 11/16″ or less but more than 5/8″ 2,500 5,000 (2) 5/8″ or less...

  10. Synthesis of 2,15-Hexadecanedione as a Precursor of Muscone

    Institute of Scientific and Technical Information of China (English)

    郭媛; 顾焕; 史真

    2005-01-01

    Muscone is a precious fragrant compound scarce in nature. Many synthetic attempts for this unique natural product have been carried out. In this work, the one-carbon unit transfer reaction of tetrahydrofolate coenzyme was initialed. Bisbenzimidazolium salt was used as tetrahydrofolate coenzyme model, and thus the biomimetic synthesis of 2,15-hexadecanedione, a precursor of muscone, was successfully accomplished by using the addition-hydrolysis reaction of bisbenzimidazolium salt with methyl magnesium iodide.

  11. Modal Interpolation Program, L 215 (INTERP). Volume 2; Supplemental System Design and Maintenance Document

    Science.gov (United States)

    Hirayama, M. Y.; Kroll, R. I.; Clemmons, R. E.

    1979-01-01

    The design, structure, and usage of the modal interpolation program L215 are presented. The program uses modal data sets of arrays containing interpolation coefficients. The interpolation arrays are used to determine displacements at various aerodynamic control points. The displacements consist of translations normal to the aerodynamic surface and surface slopes that are parallel and perpendicular to the free stream direction. Five different interpolation methods are available.

  12. Oxidative stress and mitochondrial functions in the intestinal Caco-2/15 cell line.

    Directory of Open Access Journals (Sweden)

    Rame Taha

    Full Text Available BACKGROUND: Although mitochondrial dysfunction and oxidative stress are central mechanisms in various pathological conditions, they have not been extensively studied in the gastrointestinal tract, which is known to be constantly exposed to luminal oxidants from ingested foods. Key among these is the simultaneous consumption of iron salts and ascorbic acid, which can cause oxidative damage to biomolecules. METHODOLOGY/PRINCIPAL FINDINGS: The objective of the present work was to evaluate how iron-ascorbate (FE/ASC-mediated lipid peroxidation affects mitochondrion functioning in Caco-2/15 cells. Our results show that treatment of Caco-2/15 cells with FE/ASC (0.2 mM/2 mM (1 increased malondialdehyde levels assessed by HPLC; (2 reduced ATP production noted by luminescence assay; (3 provoked dysregulation of mitochondrial calcium homeostasis as evidenced by confocal fluorescence microscopy; (4 upregulated the protein expression of cytochrome C and apoptotic inducing factor, indicating exaggerated apoptosis; (5 affected mitochondrial respiratory chain complexes I, II, III and IV; (6 elicited mtDNA lesions as illustrated by the raised levels of 8-OHdG; (7 lowered DNA glycosylase, one of the first lines of defense against 8-OHdG mutagenicity; and (8 altered the gene expression and protein mass of mitochondrial transcription factors (mtTFA, mtTFB1, mtTFB2 without any effects on RNA Polymerase. The presence of the powerful antioxidant BHT (50 microM prevented the occurrence of oxidative stress and most of the mitochondrial abnormalities. CONCLUSIONS/SIGNIFICANCE: Collectively, our findings indicate that acute exposure of Caco-2/15 cells to FE/ASC-catalyzed peroxidation produces harmful effects on mitochondrial functions and DNA integrity, which are abrogated by the powerful exogenous BHT antioxidant. Functional derangements of mitochondria may have implications in oxidative stress-related disorders such as inflammatory bowel diseases.

  13. A possible Chandra and Hubble Space Telescope detection of extragalactic WHIM towards PG 1116+215

    CERN Document Server

    Bonamente, M; Tilton, E; Liivamagi, J; Tempel, E; Heinamaki, P; Fang, T

    2016-01-01

    (Abridged) We have analyzed Chandra LETG and XMM-Newton RGS spectra towards the z=0.177 quasar PG 1116+215, a sightline that is rendered particularly interesting by the HST detection of several OVI and HI broad Lyman-alpha absorption lines that may be associated with the warm-hot intergalactic medium. We performed a search for resonance K-alpha absorption lines from OVII and OVIII at the redshifts of the detected far-ultraviolet lines. We detected an absorption line in the Chandra spectra at 5.2 sigma confidence level at wavelengths corresponding to OVIII K-alpha at z=0.0911+-0.0004+-0.0005 (statistical followed by systematic error). This redshift is within 3 sigma of that of a HI broad Lyman-alpha of b=130 km/s at z=0.09279+-0.00005. We have also analyzed the available XMM-Newton RGS data towards PG 1116+215. Unfortunately, the XMM-Newton data are not suitable to investigate this line because of instrumental features at the wavelengths of interest. At the same redshift, the Chandra and XMM-Newton spectra hav...

  14. Mechanism of the Anticoagulant Activity of Thrombin Mutant W215A/E217A

    Energy Technology Data Exchange (ETDEWEB)

    Gandhi, Prafull S.; Page, Michael J.; Chen, Zhiwei; Bush-Pelc, Leslie; Di Cera, Enrico; (WU-MED)

    2009-09-15

    The thrombin mutant W215A/E217A (WE) is a potent anticoagulant both in vitro and in vivo. Previous x-ray structural studies have shown that WE assumes a partially collapsed conformation that is similar to the inactive E* form, which explains its drastically reduced activity toward substrate. Whether this collapsed conformation is genuine, rather than the result of crystal packing or the mutation introduced in the critical 215-217 {beta}-strand, and whether binding of thrombomodulin to exosite I can allosterically shift the E* form to the active E form to restore activity toward protein C are issues of considerable mechanistic importance to improve the design of an anticoagulant thrombin mutant for therapeutic applications. Here we present four crystal structures of WE in the human and murine forms that confirm the collapsed conformation reported previously under different experimental conditions and crystal packing. We also present structures of human and murine WE bound to exosite I with a fragment of the platelet receptor PAR1, which is unable to shift WE to the E form. These structural findings, along with kinetic and calorimetry data, indicate that WE is strongly stabilized in the E* form and explain why binding of ligands to exosite I has only a modest effect on the E*-E equilibrium for this mutant. The E* {yields} E transition requires the combined binding of thrombomodulin and protein C and restores activity of the mutant WE in the anticoagulant pathway.

  15. Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr(215) in Aerococcus viridans lactate oxidase.

    Science.gov (United States)

    Stoisser, Thomas; Brunsteiner, Michael; Wilson, David K; Nidetzky, Bernd

    2016-01-01

    L-Lactate oxidase (LOX) belongs to a large family of flavoenzymes that catalyze oxidation of α-hydroxy acids. How in these enzymes the protein structure controls reactivity presents an important but elusive problem. LOX contains a prominent tyrosine in the substrate binding pocket (Tyr(215) in Aerococcus viridans LOX) that is partially responsible for securing a flexible loop which sequesters the active site. To characterize the role of Tyr(215), effects of substitutions of the tyrosine (Y215F, Y215H) were analyzed kinetically, crystallographically and by molecular dynamics simulations. Enzyme variants showed slowed flavin reduction and oxidation by up to 33-fold. Pyruvate release was also decelerated and in Y215F, it was the slowest step overall. A 2.6-Å crystal structure of Y215F in complex with pyruvate shows the hydrogen bond between the phenolic hydroxyl and the keto oxygen in pyruvate is replaced with a potentially stronger hydrophobic interaction between the phenylalanine and the methyl group of pyruvate. Residues 200 through 215 or 216 appear to be disordered in two of the eight monomers in the asymmetric unit suggesting that they function as a lid controlling substrate entry and product exit from the active site. Substitutions of Tyr(215) can thus lead to a kinetic bottleneck in product release. PMID:27302031

  16. Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr215 in Aerococcus viridans lactate oxidase

    Science.gov (United States)

    Stoisser, Thomas; Brunsteiner, Michael; Wilson, David K.; Nidetzky, Bernd

    2016-01-01

    L-Lactate oxidase (LOX) belongs to a large family of flavoenzymes that catalyze oxidation of α-hydroxy acids. How in these enzymes the protein structure controls reactivity presents an important but elusive problem. LOX contains a prominent tyrosine in the substrate binding pocket (Tyr215 in Aerococcus viridans LOX) that is partially responsible for securing a flexible loop which sequesters the active site. To characterize the role of Tyr215, effects of substitutions of the tyrosine (Y215F, Y215H) were analyzed kinetically, crystallographically and by molecular dynamics simulations. Enzyme variants showed slowed flavin reduction and oxidation by up to 33-fold. Pyruvate release was also decelerated and in Y215F, it was the slowest step overall. A 2.6-Å crystal structure of Y215F in complex with pyruvate shows the hydrogen bond between the phenolic hydroxyl and the keto oxygen in pyruvate is replaced with a potentially stronger hydrophobic interaction between the phenylalanine and the methyl group of pyruvate. Residues 200 through 215 or 216 appear to be disordered in two of the eight monomers in the asymmetric unit suggesting that they function as a lid controlling substrate entry and product exit from the active site. Substitutions of Tyr215 can thus lead to a kinetic bottleneck in product release. PMID:27302031

  17. 20 CFR 652.215 - Do any provisions in WIA change the requirement that State merit-staff employees must deliver...

    Science.gov (United States)

    2010-04-01

    ... requirement that State merit-staff employees must deliver services provided under the Act? 652.215 Section 652... § 652.215 Do any provisions in WIA change the requirement that State merit-staff employees must deliver... authority of the Act, including services to veterans, be provided by State merit-staff employees....

  18. 48 CFR 215.403-1 - Prohibition on obtaining cost or pricing data (10 U.S.C. 2306a and 41 U.S.C. 254b).

    Science.gov (United States)

    2010-10-01

    ... METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 215.403-1 Prohibition on obtaining... clearly established on the basis of price analysis (see FAR 15.404-1(b)). (3) Commercial items. (A) Follow the procedures at PGI 215.403-1(c)(3)(A) for pricing commercial items. (B) By November 30th of...

  19. 48 CFR 52.215-20 - Requirements for Certified Cost or Pricing Data and Data Other Than Certified Cost or Pricing Data.

    Science.gov (United States)

    2010-10-01

    ... Certificate of Current Cost or Pricing Data, as prescribed by FAR 15.406-2. (End of provision) Alternate I... Cost or Pricing Data and Data Other Than Certified Cost or Pricing Data. 52.215-20 Section 52.215-20... Certified Cost or Pricing Data and Data Other Than Certified Cost or Pricing Data. As prescribed in...

  20. 不明原因长期发热215例病因分析

    Institute of Scientific and Technical Information of China (English)

    杨明珍; 胡红琳; 刘爽; 蔡学杰

    2003-01-01

    @@ 不明原因长期发热(fever of unknown origin, FUO)是指发热持续3周以上,体温≥38.5℃,经完整的病史询问、体检及常规实验室检查后仍不能明确诊断者[1].FUO是内科临床疑难病症,其病因复杂,易引起误诊和漏诊.我们对近10年来收治我院内科215例FUO病例进行回顾性病因分析.

  1. Space charge in drift chambers operated with the Xe, CO$_{2}$(15\\%) mixture

    CERN Document Server

    Andronic, A; Blume, C; Braun-Munzinger, P; Bucher, D; Busch, O; Catanescu, V; Ciobanu, M; Daues, H W; Emschermann, D; Fateev, O V; Foka, Y; Garabatos, C; Gunji, T; Herrmann, N; Inuzuka, M; Kislov, E; Lindenstruth, V; Ludolphs, W; Mahmoud, T; Petracek, V; Petrovici, M; Rusanov, I R; Sandoval, A; Santo, R; Schicker, R; Simon, R S; Smykov, L P; Soltveit, H K; Stachel, J; Stelzer, H; Tsiledakis, G; Vulpescu, B; Wessels, J P; Windelband, B; Xu, C; Zaudtke, O; Zanevsky, Yu; Yurevich, V

    2004-01-01

    Using prototype modules of the ALICE Transition Radiation Detector (TRD) we investigate space-charge effects and the dependence of the pion rejection performance on the incident angle of the ionizing particle. The average pulse height distributions in the drift chambers operated with the Xe, CO //2(15%) mixture provide quantitative information on the gas gain reduction due to space charge accumulating during the drift of the primary ionization. Our results demonstrate that the pion rejection performance of a TRD is better for tracks which are not at normal incidence to the anode wires. We present detailed simulations of detector signals, which reproduce the measurements and lend strong support to our interpretation of the measurements in terms of space-charge effects.

  2. miR-215 overexpression distinguishes ampullary carcinomas from pancreatic carcinomas.

    Science.gov (United States)

    Choi, Dong Ho; Park, Sang Jae; Kim, Hark Kyun

    2015-06-01

    Distinguishing ampullary carcinoma from pancreatic carcinoma is important because of their different prognoses. microRNAs are differentially expressed according to the tissue of origin. However, there is rare research on the differential diagnosis between the two types of cancers by microRNA in periampullary cancers. The present study was undertaken to compare microRNA profiles between ampullary and pancreatic carcinomas using microarrays. miR-215 was most significantly overexpressed in ampullary carcinomas; whereas the expressions of miR-134 and miR-214 were significantly lower in ampullary carcinomas than in pancreatic carcinomas. When these discriminatory microRNAs were applied to liver metastases, they were correctly predicted for the tissue of origin. Although this study is limited by small sample size, striking difference in microRNA expression and concordant expression of discriminating microRNAs in primary tumors and metastases suggest that these novel discriminatory microRNAs warrant future validation.

  3. 1 215例次恶性胆管梗阻内镜治疗的体会%Endoscopic management of 1 215 malignant bile duct obstructions

    Institute of Scientific and Technical Information of China (English)

    胡冰; 周岱云; 龚彪; 王书智; 杨广顺; 吴孟超

    2001-01-01

    目的探讨采用治疗性内窥镜逆行胰胆管插管技术(ERCP技术),姑息性解除恶性肿瘤所致胆管梗阻的可行性。方法采用ERCP治疗929例1 215例次胆管恶性梗阻,包括胆管癌567例次、肝癌255例次、胆囊癌83例次、胰腺癌172例次、乳头壶腹癌97例次、其它转移性肿瘤41例次;导致胆管低位梗阻263例次、中段梗阻43例次、高位梗阻909例次;血清胆红素(284.3±174.5)μmol/L。共行胆道内置管引流599例次、鼻胆管引流385例次,放置可膨式金属胆道支架167例次,联合引流39例次,乳头括约肌切开19例次,乳头开窗造口术6例次。结果操作成功率94.3%,消除黄疸总有效率81.8%,治疗后患者平均生存14个月,1、2、3年的预计存活率分别为75.9%、44.0%和25.2%。全组发生与操作有关并发症23例(2.6%),死亡3例(0.2%)。结论内镜介入治疗技术是安全有效解除胆道恶性梗阻的方法,对中晚期胆道肿瘤患者基本可替代姑息性胆道手术。%Objective To review the experience with endoscopic palliation of malignant obstructive jaundice (MOJ) in 929 patients. Methods From 1994 to 1998, 929 patients (598 Males and 331 females) underwent 1 215 endoscopic palliation drainages for MOJ in our hospital. The mean age of these patients was 57 years (range 8-86). Tumor obstruction occurred in the distal CBD (263 patients), the middle CBD (43), and the hilum (909), with a mean bilirubin level of 284 μmol/L (range 26-810). Of the 1 215 drainages, 599 were performed by stenting with plastic endoprosthesis, 385 by nasobiliary catheterization, 167 by expandable metal stents, 39 by combined drainage methods, 19 by tumor papillotomy, and 6 by endoscopic fistulostomy.  Results The success rate of endoscopic procedure was 94.3%. Jaundice was inproved in 81.8% of the patients with a significant reduction of serum bilirubin (64.7%). Post-drainage complication rate was 2.6%; which

  4. 40 CFR 1068.215 - What are the provisions for exempting manufacturer-owned engines/equipment?

    Science.gov (United States)

    2010-07-01

    ... PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS GENERAL COMPLIANCE PROVISIONS FOR ENGINE PROGRAMS... least the following items: (i) The label heading “EMISSION CONTROL INFORMATION”. (ii) Your corporate... ENGINE IS EXEMPT UNDER 40 CFR 1068.210 OR 1068.215 FROM EMISSION STANDARDS AND RELATED REQUIREMENTS.”...

  5. Zum 215. Gebutstag von Admiral Ferdinand Baron von Wrangell : (29. Dezember 1797 - 25. 25. Mai 1870) / Peter Krienitz

    Index Scriptorium Estoniae

    Krienitz, Peter

    2012-01-01

    Admiral Ferdinand Baron von Wrangellist tema 215. sünniaastapäeva puhul. Silmapaistavast Kirde-Siberi uurijast. Tema poliitilisest tegevusest ja elust Roela mõisas. Ferdinand Baroni poolt seatud viisil tähistati jõulusid Roelas kuni 1944. aasta lõpuni.

  6. Foco Nasmyth para el telescopio 2,15mts. de CASLEO

    Science.gov (United States)

    Casagrande, A. R.

    En principio, este proyecto intenta lograr el mayor aprovechamiento posible del instrumental que se dispone, buscando la manera de optimizar y hacer más eficiente el servicio que brinda el CASLEO a la comunidad astronómica. El mismo consiste en utilizar dispositivos ya existentes en el telescopio, y darle una utilidad. Tal es el caso del camino óptico destinado al foco Coude. Si tenemos en cuenta que disponemos de un tercer espejo Coude, con todos sus mecanismos automatizados, (actualmente sin uso), una distancia apropiada del plano focal, el espacio y el lugar físico necesario para instalar un periférico, es posible la habilitación de un foco Nasmyth en el telescopio 2,15mts. El hecho de contar con este nuevo foco, redundará en importantes beneficios. En primer lugar, posibilitará la observación, casi simultánea, con dos instrumentos. Otro aspecto a tener en cuenta, es que disminuirá el frecuente cambio del instrumental periférico, motivo este que degrada su ideal puesta a punto. Por último, también de interés, es de destacar su escaso costo de ejecución.

  7. Biopsy of human morula-stage embryos: outcome of 215 IVF/ICSI cycles with PGS.

    Directory of Open Access Journals (Sweden)

    Elena E Zakharova

    Full Text Available Preimplantation genetic diagnosis (PGD is commonly performed on biopsies from 6-8-cell-stage embryos or blastocyst trophectoderm obtained on day 3 or 5, respectively. Day 4 human embryos at the morula stage were successfully biopsied. Biopsy was performed on 709 morulae from 215 ICSI cycles with preimplantation genetic screening (PGS, and 3-7 cells were obtained from each embryo. The most common vital aneuploidies (chromosomes X/Y, 21 were screened by fluorescence in situ hybridization (FISH. No aneuploidy was observed in 72.7% of embryos, 91% of those developed to blastocysts. Embryos were transferred on days 5-6. Clinical pregnancy was obtained in 32.8% of cases, and 60 babies were born. Patients who underwent ICSI/PGS treatment were compared with those who underwent standard ICSI treatment by examining the percentage of blastocysts, pregnancy rate, gestational length, birth height and weight. No significant differences in these parameters were observed between the groups. Day 4 biopsy procedure does not adversely affect embryo development in vitro or in vivo. The increased number of cells obtained by biopsy of morulae might facilitate diagnostic screening. There is enough time after biopsy to obtain PGD results for embryo transfer on day 5-6 in the current IVF cycle.

  8. Autoguía para el telescopio 2,15 mts de CASLEO

    Science.gov (United States)

    Aballay, J. A.; Casagrande, A. R.; Pereyra, P. F.; Marún, A. H.

    Se está desarrollando un sistema de autoguía para el telescopio de 2,15 mts. El mismo se realizará aprovechando el Offset Guider. Al ocular móvil de éste se vinculará alguna cámara digital (ST4-ST7-CH250) para lograr la visión del objeto. El funcionamiento del equipo será el siguiente: primero, dadas las coordenadas del objeto a observar, se tomarán las coordenadas del telescopio para que, a través de una base de datos, se determine un campo de objetos que sirvan para la cámara de visión, luego, la PC obtendrá el offset entre la estrella de observación y la estrella seleccionada como guía, este valor será trasladado a los motores que posicionarán en forma automática el ocular. Una vez que la estrella es visualizada en la cámara (monitor de PC ) se correrá el programa que guiará el telescopio automáticamente.

  9. Improved energy of the 21.5 keV M1 + E2 nuclear transition in {sup 151}Eu

    Energy Technology Data Exchange (ETDEWEB)

    Inoyatov, A.Kh. [JINR, Laboratory of Nuclear Problems, Dubna, Moscow Region (Russian Federation); National University, Institute of Applied Physics, Tashkent (Uzbekistan); Kovalik, A. [JINR, Laboratory of Nuclear Problems, Dubna, Moscow Region (Russian Federation); Nuclear Physics Institute of the ASCR, Rez near Prague (Czech Republic); Filosofov, D.V.; Perevoshchikov, L.L. [JINR, Laboratory of Nuclear Problems, Dubna, Moscow Region (Russian Federation); Rysavy, M. [Nuclear Physics Institute of the ASCR, Rez near Prague (Czech Republic); Baimukhanova, A. [JINR, Laboratory of Nuclear Problems, Dubna, Moscow Region (Russian Federation); Institute of Nuclear Physics, Almaty (Kazakhstan)

    2016-05-15

    Using internal conversion electron spectroscopy, improved energy 21 541.5±0.5 eV was determined for the 21.5 keV M1+E2 nuclear transition in {sup 151}Eu populated in the electron capture decay of {sup 151}Gd. This value was found to agree well with the present adopted value but is much more accurate. A value of 0.0305±0.0011 derived for the E2 admixture parameter vertical stroke δ(E2/M1) vertical stroke from the measured conversion electron line intensities corresponds to the present adopted value. A possible effect of nuclear structure on the multipolarity of the 21.5 keV transition was also investigated. (orig.)

  10. Measurement of regional cerebral blood flow with H215O positron emission tomography during Matas test - report of three cases

    International Nuclear Information System (INIS)

    The authors carried out a Matas test with a regional cerebral blood flow (rCBF) study using H215O positron emission tomography (PET) for three cases of large internal carotid artery aneurysms. There is a likely correlation between the cerebral blood flow (CBF) reduction rate obtained by PET, and the mean stump pressure available from a conventional balloon occlusion test. The advantages of this noninvasive and quantitative method are presented in comparison with other methods. (author)

  11. 215例血友病A患者因子Ⅷ抑制物形成的环境因素研究%Environmental factors for inhibitor formation of factor Ⅷ in 215 patients with hemophilia A

    Institute of Scientific and Technical Information of China (English)

    周璇; 孙竞; 李敏; 李文卿; 宋晓玲; 刘阳

    2011-01-01

    目的 研究中国血友病A患者因子Ⅷ(FⅧ)抑制物形成的环境因素.方法 监测215例血友病A患者在2年(2007年6月至2009年6月)的连续随访中,FⅧ抑制物发生、变化及转归,并对FⅧ抑制物形成的环境因素进行回顾性分析.结果 215例血友病A患者随访2年FⅧ抑制物累积发生率为11.6%(25/215).FⅧ抑制物形成的环境因素的多变量分析结果示:①输注原因中短期低剂量预防治疗比按需治疗形成FⅧ抑制物的风险低(OR=0.037,95%CI为 0.002~0.616); ②血友病越严重,累积暴露日越少,FⅧ抑制物形成风险越高;③发生严重出血事件是FⅧ抑制物形成的高危因素(OR=117.045,95%CI为19.333~708.617); ④输注形式和发生重大感染事件与FⅧ抑制物形成无关.结论 在中国目前治疗情况下,血友病患者 FⅧ抑制物的形成可能与血友病严重程度、输注原因(预防治疗或按需治疗)、累积暴露日和是否发生严重出血事件有关.%Objective To study the environmental factors for inhibitor formation of factor Ⅷ in 215 Chinese patients with hemophilia A. Methods Formation, change and transformation of factor Ⅷ were observed in 215 Chinese patients with hemophilia A during the 2-year follow-up period from June 2007 to June 2009. The environmental factors for inhibitor formation of factor Ⅷ were retrospectively analyzed. Results The cumulative incidence of hemophilia A in the 215 Chinese patients during the 2-year follow-up period was 11.6% (25/215). Multivariate analysis showed that the risk for inhibitor formation of factor Ⅷ was lower in short-term low-dose preventive treatment than in on-demand treatment (OR =0. 037, 95% CI =0. 002 -0.616). The higher the severity of hemophilia was, the less the cumulative exposure day was, and the greater the risk for the inhibitor formation of factor Ⅷ was. Severe bleeding episodes was a high risk factor for the inhibitor formation of factor Ⅷ ( OR = 117. 045

  12. microRNA-215异常激活抑制Dicer1促进肝癌细胞迁移和转化

    Institute of Scientific and Technical Information of China (English)

    李文娟; 韩翰; 孙丹; 沈洪星; 李友军; 张进祥

    2015-01-01

    microRNA异常表达促进癌症的发生发展.本研究通过microRNA表达谱分析2个肝癌细胞和2个正常细胞microRNA的表达,寻找与肝癌相关的microRNA,发现microRNA-215在肝癌细胞中高表达,qRT—PCR验证microRNA-215在肝癌细胞呈显著高表达.进一步研究发现,microRNA-215直接靶向Dicerl基因的3'UTR并抑制Dicerl蛋白表达,Dicerl是microRNA加工成熟过程中必需的蛋白.过表达microRNA-215抑制Dicerl从而促进肝癌细胞迁移和转化,而抑制microRNA-215表达起相反作用.Dicerl抑制后,许多抑癌microRNA表达被抑制,从而促进迁移和转化.相对于癌旁组织,Dicerl在肝癌组织呈明显低表达.本研究揭示,microRNA-215异常活化并抑制Dicerl表达与肝癌发展相关.

  13. Hepatitis B virus X protein mutant HBxΔ127 promotes proliferation of hepatoma cells through up-regulating miR-215 targeting PTPRT

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Fabao [Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin 300071 (China); Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China); You, Xiaona [Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin 300071 (China); Chi, Xiumei [Department of Hepatology, The First Hospital, Jilin University, Changchun 130021 (China); Wang, Tao [Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin 300071 (China); Ye, Lihong [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China); Niu, Junqi, E-mail: junqiniu@yahoo.com.cn [Department of Hepatology, The First Hospital, Jilin University, Changchun 130021 (China); Zhang, Xiaodong, E-mail: zhangxd@nankai.edu.cn [Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin 300071 (China)

    2014-02-07

    Highlights: • Relative to wild type HBx, HBX mutant HBxΔ127 strongly enhances cell proliferation. • Relative to wild type HBx, HBxΔ127 remarkably up-regulates miR-215 in hepatoma cells. • HBxΔ127-elevated miR-215 promotes cell proliferation via targeting PTPRT mRNA. - Abstract: The mutant of virus is a frequent event. Hepatitis B virus X protein (HBx) plays a vital role in the development of hepatocellular carcinoma (HCC). Therefore, the identification of potent mutant of HBx in hepatocarcinogenesis is significant. Previously, we identified a natural mutant of the HBx gene (termed HBxΔ127). Relative to wild type HBx, HBxΔ127 strongly enhanced cell proliferation and migration in HCC. In this study, we aim to explore the mechanism of HBxΔ127 in promotion of proliferation of hepatoma cells. Our data showed that both wild type HBx and HBxΔ127 could increase the expression of miR-215 in hepatoma HepG2 and H7402 cells. However, HBxΔ127 was able to significantly increase miR-215 expression relative to wild type HBx in the cells. We identified that protein tyrosine phosphatase, receptor type T (PTPRT) was one of the target genes of miR-215 through targeting 3′UTR of PTPRT mRNA. In function, miR-215 was able to promote the proliferation of hepatoma cells. Meanwhile anti-miR-215 could partially abolish the enhancement of cell proliferation mediated by HBxΔ127 in vitro. Knockdown of PTPRT by siRNA could distinctly suppress the decrease of cell proliferation mediated by anti-miR-215 in HepG2-XΔ127/H7402-XΔ127 cells. Moreover, we found that anti-miR-215 remarkably inhibited the tumor growth of hepatoma cells in nude mice. Collectively, relative to wild type HBx, HBxΔ127 strongly enhances proliferation of hepatoma cells through up-regulating miR-215 targeting PTPRT. Our finding provides new insights into the mechanism of HBx mutant HBxΔ127 in promotion of proliferation of hepatoma cells.

  14. Superpave 在青海省 G215 大察公路上的应用

    Institute of Scientific and Technical Information of China (English)

    彭艳丽

    2008-01-01

    该文介绍了Superpave的发展应用情况及其特点,并结合Superpave在青海省G215大察公路上的应用情况,介绍了Superpave的配合比设计方法、施工技术以及应用效果,为今后推广使用Superpave沥青路面,提高沥青路面施工质量提供借鉴.

  15. Crystal structure of spinel-type Li0.64Fe2.15Ge0.21O4

    OpenAIRE

    Günther J. Redhammer; Gerold Tippelt

    2016-01-01

    Spinel-type Li0.64Fe2.15Ge0.21O4, lithium diiron(III) germanium tetraoxide, has been formed as a by-product during flux growth of an Li–Fe–Ge pyroxene-type material. In the title compound, lithium is ordered on the octahedral B sites, while Ge4+ orders onto the tetrahedral A sites, and iron distributes over both the octahedral and tetrahedral sites, and is in the trivalent state as determined from Mössbauer spectroscopy. The oxygen parameter u is 0.2543; thus, the spinel is close to having an...

  16. 外周血单个核细胞与HepG2.215细胞共培养体系的研究%Study of peripheral blood mononuclear cells and HepG2.215 cells co-culture system

    Institute of Scientific and Technical Information of China (English)

    胡勇; 吴晓蔓

    2011-01-01

    目的 建立外周血单个核细胞与HepG2.215细胞共培养体系,探讨不同培养基及效靶比对共培养体系的影响.方法 分离正常人外周血单个核细胞,加人植物血凝素(PHA),置于不同的培养基(DMEM,RPMI1640)中进行培养,采用不同效靶比((5:1、10:1、20:1、40:1)构建PBMCs与HepG2.215细胞共培养体系.用倒置显微镜观察细胞的形态及生长情况,台盼蓝拒染法检测PBMCs与HepG2.215细胞的细胞活力,cck-8法检测HepG2.215细胞的增殖活性.结果 DMEM培养基培养的HepG2.215细胞的细胞活力比RPM11640培养基培养的细胞高;而两种培养基培养的PBMCs的细胞活力无明显变化.共培养条件下,PBMCs对HepG2.215细胞增殖活性的抑制作用随效靶比的不同而有所差别,效靶比为20:1时抑制作用最强.结论 在PBMCs与HepG2.215细胞共培养体系中,细胞培养基和效靶比对HepG2.215细胞的生长有影响.%Objective To establish the co-culture system of peripheral blood mononuclear cells and HepG2.215 cells,and explore the effects of different culture medium and the target ratio on the impact of co-culture system. Methods PBMCs separated from normal human blood were cultured with PHA in different mediums (DMEM, RPMI1640).The co-culture system of PBMCs and HepG2.215 cells was established in different proportion (5:1,10:1,20:1,40:1 ).Morphology and growth condition of cells were observed by inverted microscope. The activity of PBMCs and HepG2.215 cells was evaluated by Trypan blue staining. The proliferation of HepG2.215 cells was measured by cck-8. Results The activity of HepG2.215 cells cultured with DMEM was higher than that of cells cultured with RPMI 1640(P<0.05). The activity of PBMCs had no significant change.The inhibition of PBMCs on HepG2.215 cells changed with the ratio of effector cells and target cells in co-culture condition,and shoed the strongest inhibition when the target ratio was 20:1. Conclusion In the PBMCs and HepG2.215

  17. Limited ability of humoral immune responses in control of viremia during infection with SIVsmmD215 strain

    Energy Technology Data Exchange (ETDEWEB)

    Ribiero, Ruy M [Los Alamos National Laboratory

    2009-01-01

    To investigate the impact of humoral immunity on SIV replication, 11 rhesus macaques (RMs) were inoculated with the neutralization-sensitive strain SIVsmmD215. Seven RMs were treated every three weeks, with 50 mglkg of an anti-CD20 antibody (Rituxan, gift from Genentech) starting from day -7 p.i., as follows: four RMs were treated for two months, and three were treated for five months. The remaining four RMs were used as controls. Three RMs were completely depleted of CD20 cells. Four RMs only partially depleted CD20 cells in the LNs and intestine. The efficacy of tissue CD20 depletion predicted the ablation of antibody production, with SIVsmm seroconversion being delayed in the animals with complete tissue CD20 depletion, and neutralizing antibody production being significantly delayed and at low levels in all CD20-depleted RMs. There was no significant difference in acute or chronic VLs between CD20-depleted RMs and control monkeys, with a tendency for lower set-point VLs in CD20-depleted RMs. At 6 weeks p.i., cellular immune responses were significantly stronger in CD20 depleted RMs than in controls. After two years p.i., there was no significant difference in survival between CD20-depleted and control RMs. We concluded that CD20 depletion plays no significant role in the control of SIV replication or disease progression in SIVsmmD215-infected RMs.

  18. Analysis of the Zidovudine Resistance Mutations T215Y, M41L, and L210W in HIV-1 Reverse Transcriptase.

    Science.gov (United States)

    Boyer, Paul L; Das, Kalyan; Arnold, Eddy; Hughes, Stephen H

    2015-12-01

    Although anti-human immunodeficiency virus type 1 (HIV-1) therapies have become more sophisticated and more effective, drug resistance continues to be a major problem. Zidovudine (azidothymidine; AZT) was the first nucleoside reverse transcriptase (RT) inhibitor (NRTI) approved for the treatment of HIV-1 infections and is still being used, particularly in the developing world. This drug targets the conversion of single-stranded RNA to double-stranded DNA by HIV-1 RT. However, resistance to the drug quickly appeared both in viruses replicating in cells in culture and in patients undergoing AZT monotherapy. The primary resistance pathway selects for mutations of T215 that change the threonine to either a tyrosine or a phenylalanine (T215Y/F); this resistance pathway involves an ATP-dependent excision mechanism. The pseudo-sugar ring of AZT lacks a 3' OH; RT incorporates AZT monophosphate (AZTMP), which blocks the end of the viral DNA primer. AZT-resistant forms of HIV-1 RT use ATP in an excision reaction to unblock the 3' end of the primer strand, allowing its extension by RT. The T215Y AZT resistance mutation is often accompanied by two other mutations, M41L and L210W. In this study, the roles of these mutations, in combination with T215Y, were examined to determine whether they affect polymerization and excision by HIV-1 RT. The M41L mutation appears to help restore the DNA polymerization activity of RT containing the T215Y mutation and also enhances AZTMP excision. The L210W mutation plays a similar role, but it enhances excision by RTs that carry the T215Y mutation when ATP is present at a low concentration. PMID:26324274

  19. MiR-21-5p Links Epithelial-Mesenchymal Transition Phenotype with Stem-Like Cell Signatures via AKT Signaling in Keloid Keratinocytes

    Science.gov (United States)

    Yan, Li; Cao, Rui; Liu, YuanBo; Wang, LianZhao; Pan, Bo; Lv, XiaoYan; Jiao, Hu; Zhuang, Qiang; Sun, XueJian; Xiao, Ran

    2016-01-01

    Keloid is the abnormal wound healing puzzled by the aggressive growth and high recurrence rate due to its unrevealed key pathogenic mechanism. MicroRNAs contribute to a series of biological processes including epithelial-mesenchymal transition (EMT) and cells stemness involved in fibrotic disease. Here, using microRNAs microarray analysis we found mir-21-5p was significantly up-regulated in keloid epidermis. To investigate the role of miR-21-5p in keloid pathogenesis, we transfected miR-21-5p mimic or inhibitor in keloid keratinocytes and examined the abilities of cell proliferation, apoptosis, migration and invasion, the expressions of EMT-related markers vimentin and E-cadherin and stem-like cells-associated markers CD44 and ALDH1, and the involvement of PTEN and the signaling of AKT and ERK. Our results demonstrated that up-regulation or knockdown of miR-21-5p significantly increased or decreased the migration, invasion and sphere-forming abilities of keloid keratinocytes, and the phenotype of EMT and cells stemness were enhanced or reduced as well. Furthermore, PTEN and p-AKT were shown to participate in the regulation of miR-21-5p on EMT phenotypes and stemness signatures of keloid keratinocytes, which might account for the invasion and recurrence of keloids. This molecular mechanism of miR-21-5p on keloid keratinocytes linked EMT with cells stemness and implicated novel therapeutic targets for keloids. PMID:27596120

  20. MiR-21-5p Links Epithelial-Mesenchymal Transition Phenotype with Stem-Like Cell Signatures via AKT Signaling in Keloid Keratinocytes

    Science.gov (United States)

    Yan, Li; Cao, Rui; Liu, Yuanbo; Wang, Lianzhao; Pan, Bo; Lv, Xiaoyan; Jiao, Hu; Zhuang, Qiang; Sun, Xuejian; Xiao, Ran

    2016-09-01

    Keloid is the abnormal wound healing puzzled by the aggressive growth and high recurrence rate due to its unrevealed key pathogenic mechanism. MicroRNAs contribute to a series of biological processes including epithelial-mesenchymal transition (EMT) and cells stemness involved in fibrotic disease. Here, using microRNAs microarray analysis we found mir-21-5p was significantly up-regulated in keloid epidermis. To investigate the role of miR-21-5p in keloid pathogenesis, we transfected miR-21-5p mimic or inhibitor in keloid keratinocytes and examined the abilities of cell proliferation, apoptosis, migration and invasion, the expressions of EMT-related markers vimentin and E-cadherin and stem-like cells-associated markers CD44 and ALDH1, and the involvement of PTEN and the signaling of AKT and ERK. Our results demonstrated that up-regulation or knockdown of miR-21-5p significantly increased or decreased the migration, invasion and sphere-forming abilities of keloid keratinocytes, and the phenotype of EMT and cells stemness were enhanced or reduced as well. Furthermore, PTEN and p-AKT were shown to participate in the regulation of miR-21-5p on EMT phenotypes and stemness signatures of keloid keratinocytes, which might account for the invasion and recurrence of keloids. This molecular mechanism of miR-21-5p on keloid keratinocytes linked EMT with cells stemness and implicated novel therapeutic targets for keloids.

  1. MiR-21-5p Links Epithelial-Mesenchymal Transition Phenotype with Stem-Like Cell Signatures via AKT Signaling in Keloid Keratinocytes.

    Science.gov (United States)

    Yan, Li; Cao, Rui; Liu, YuanBo; Wang, LianZhao; Pan, Bo; Lv, XiaoYan; Jiao, Hu; Zhuang, Qiang; Sun, XueJian; Xiao, Ran

    2016-01-01

    Keloid is the abnormal wound healing puzzled by the aggressive growth and high recurrence rate due to its unrevealed key pathogenic mechanism. MicroRNAs contribute to a series of biological processes including epithelial-mesenchymal transition (EMT) and cells stemness involved in fibrotic disease. Here, using microRNAs microarray analysis we found mir-21-5p was significantly up-regulated in keloid epidermis. To investigate the role of miR-21-5p in keloid pathogenesis, we transfected miR-21-5p mimic or inhibitor in keloid keratinocytes and examined the abilities of cell proliferation, apoptosis, migration and invasion, the expressions of EMT-related markers vimentin and E-cadherin and stem-like cells-associated markers CD44 and ALDH1, and the involvement of PTEN and the signaling of AKT and ERK. Our results demonstrated that up-regulation or knockdown of miR-21-5p significantly increased or decreased the migration, invasion and sphere-forming abilities of keloid keratinocytes, and the phenotype of EMT and cells stemness were enhanced or reduced as well. Furthermore, PTEN and p-AKT were shown to participate in the regulation of miR-21-5p on EMT phenotypes and stemness signatures of keloid keratinocytes, which might account for the invasion and recurrence of keloids. This molecular mechanism of miR-21-5p on keloid keratinocytes linked EMT with cells stemness and implicated novel therapeutic targets for keloids. PMID:27596120

  2. Crystal structure of spinel-type Li0.64Fe2.15Ge0.21O4.

    Science.gov (United States)

    Redhammer, Günther J; Tippelt, Gerold

    2016-04-01

    Spinel-type Li0.64Fe2.15Ge0.21O4, lithium diiron(III) germanium tetra-oxide, has been formed as a by-product during flux growth of an Li-Fe-Ge pyroxene-type material. In the title compound, lithium is ordered on the octa-hedral B sites, while Ge(4+) orders onto the tetra-hedral A sites, and iron distributes over both the octa-hedral and tetra-hedral sites, and is in the trivalent state as determined from Mössbauer spectroscopy. The oxygen parameter u is 0.2543; thus, the spinel is close to having an ideal cubic closed packing of the O atoms. The title spinel is compared with other Li- and Ge-containing spinels. PMID:27375876

  3. A diagnostic method for suspected Alzheimer's disease using H215O positron emission tomography perfusion Z score

    International Nuclear Information System (INIS)

    We developed cerebral perfusion Z score map (Z map) images using H215O and positron emission tomography (PET), and examined their use in diagnosing Alzheimer's disease (AD). Cerebral blood flow (CBF) images were obtained using the PET and H215O autoradiographic method. The best region for normalising the CBF value to remove individual variation was determined. Then CBF images were transformed to Talairach's standard space, and each pixel value of an individual's image set was normalized to the mean value of the sensorimotor area. Based on the CBF images of 20 normal volunteers, normative mean and standard deviation (SD) CBF images were constructed. Then, each pixel value of the axial CBF images in 28 patients with probable AD and 10 further normal volunteers was converted to a Z score (Z = [normal mean - individual value]/normal SD). A Z map, showing pixels exceeding a threshold of Z score > 2 on MRI of standardised anatomical space was demonstrated. These 38 Z maps were interpreted by four radiologists. When regions in the temporoparietal area were found with Z scores > 2, the subject was diagnosed as having AD. A receiver operating characteristic (ROC) analysis was performed to compare the conventional CBF images and Z maps. The diagnostic performance of the Z map was superior to that of visual inspection of conventional CBF images (mean areas under the ROC curve of the four radiologists were 0.946 and 0.584, respectively). These results indicate that a Z map obtained in this way is superior to conventional PET for diagnosing AD. (orig.)

  4. 47 CFR 68.215 - Installation of other than “fully protected” system premises wiring that serves more than four...

    Science.gov (United States)

    2010-10-01

    ... TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Terminal Equipment Approval Procedures § 68.215 Installation of... telephone network interface(s). Fully-protected premises wiring shall be used to connect equipment entities to the public switched telephone network interface unless the provider of wireline...

  5. 48 CFR 52.215-21 - Requirements for Certified Cost or Pricing Data and Data Other Than Certified Cost or Pricing...

    Science.gov (United States)

    2010-10-01

    ... Contractor shall submit a Certificate of Current Cost or Pricing Data, as prescribed by FAR 15.406-2. (End of... Cost or Pricing Data and Data Other Than Certified Cost or Pricing Data-Modifications. 52.215-21... Requirements for Certified Cost or Pricing Data and Data Other Than Certified Cost or Pricing...

  6. Remote processing, delivery and injection of H2[15O] produced from a N2/H2 gas target using a simple and compact apparatus.

    Science.gov (United States)

    Ferrieri, R A; Alexoff, D L; Schlyer, D J; Wolf, A P

    1994-12-01

    We report here a simple apparatus for remote trapping and processing of H2[15O] produced from the N2/H2 target. The system performs a three step operation for H2[15O] delivery at the PET imaging facility which includes the following: (i) collecting the radiotracer in sterile water; (ii) adjusting preparation pH through removal of radiolytically produced ammonia, while at the same time adjusting solution isotonicity; and (iii) delivery of the radiotracer preparation to the injection syringe in a sterile and pyrogen-free form suitable for human studies. The processing apparatus is simple, can be remotely operated and fits inside a Capintec Dose Monitoring Chamber for direct measurement of accumulated radioactivity. Using this system, 300 mCi of H2[15O] (15 microA of 8 MeV D+ on target) is transferred from target through 120 m x 3.18 mm o.d. Impolene tubing to yield 100 mCi of H2[15O] which is isotonic, neutral and suitable for human studies. A remote hydraulically driven system for i.v. injection of the H2[15O] is also described. The device allows for direct measurement of syringe dose while filling, and for easy, as well as safe transport of the injection syringe assembly to the patient's bedside via a shielded delivery cart. This cart houses a hydraulic piston that allows the physician to "manually" inject the radiotracer without directly handling the syringe. PMID:7894394

  7. Patch tests and screening for new allergen in 215 cases with contact dermatitis%215例接触性皮炎斑贴试验结果及新致敏原筛查

    Institute of Scientific and Technical Information of China (English)

    郁辉; 侯占英; 徐浪锋; 陈珩; 刘芳

    2010-01-01

    Patch tests were performed in 215 cases with contact dermatitis and 59 cases with noncontact dermatitis who visited the clinic of Xili Hospital, Shenzhen.Results showed that top four allergen for them were 0.1% Merthiolate, 5% nickel sulfate, 7% aromatic compounds and 1% formaldehyde in sequence.There was significant difference in occurrence of dermatitis at the hands, trunk, extremities,face, and periumbilicus caused by these four kinds of allergen (P <0.01 ), with 0.1% Merthiolate mainly causing dermatitis at the face, hands, trunk and extremities, 5% nickel sulfate causing dermatitis at the periumbilicus, trunk and extremities, 7% aromatic compounds causing dermatitis at the hands, trunk,extremities and face, whereas 1% formaldehyde mainly causing facial dermatitis.Concentration of pentyl acetate and rosin spirit used for patch tests should be 20 % and 25 %, respectively.%对215例门诊接触性皮炎及59例非接触性皮炎患者做斑贴试验.结果列前四位的致敏原分别是0.1%硫柳汞、5%硫酸镍、7%芳香混合物、1%甲醛.这4种致敏原在手部、躯干四肢、面部和脐周的患病率差异有统计学意义(P<0.01),提示0.1%硫柳汞主要引起面部、手部、躯干及四肢皮炎,5%硫酸镍主要引起脐周和躯干及四肢皮炎,7%芳香混合物主要引起手部、躯干及四肢、面部皮炎,1%甲醛主要引起面部皮炎;乙酸戊酯、松香水的斑贴试验浓度分别为20%、25%.

  8. 215例垂体腺瘤术后患者腺垂体功能评估及替代状况分析%Outcome of postoperative hypopituitarism and hormone replacement situation in 215 patients with pituitary adenoma

    Institute of Scientific and Technical Information of China (English)

    曾梅芳; 赵曜; 王镛斐; 李益明; 蒋翠萍; 叶红英; 张烁; 何敏; 杨叶虹; 鹿斌; 胡仁明; 李士其

    2012-01-01

    Objective To investigate the outcome of postoperative hypopituitarism and hormone replacement in patients with pituitary adenoma,and to analyze the potential factors related to postoperative hypopituitarism.Methods A total of 215 postoperative patients with pituitary adenoma were analyzed.Pituitary functions( including gonadal,thyroid,and adrenal axes ) were asessed by strict criteria.Data of surgery history and hormone replacement situation were collected for statistical analysis.Results The prevalence of hypopituitarism was 54.0%,including 36.7% hypogonadism,32.6% hypothyroidism,and 28.4% hypoadrenalism.Replacements of gonadal steroid,glucocorticoid,and thyroxine were carried out in 25.6%,84.3%,and 80.6% of the cases,respectively.Univariate analysis showed that male sex and large tumor were related to hypopituitarism. Conclusion After pituitary adenomectomy,approximately half of the patients present anterior pituitary dysfunction,while quite a number of them have not been treated appropriately.%目的 了解垂体腺瘤术后患者腺垂体功能减退及其替代治疗情况,寻找与其功能减退可能相关的因素.方法 对215例就诊于华山医院内分泌科的垂体腺瘤术后患者进行垂体性腺轴、甲状腺轴、肾上腺皮质轴功能评估,收集手术病史、替代状况等资料进行统计分析.结果 存在腺垂体功能减退的患者占54.0%,其中性腺、甲状腺、肾上腺皮质功能减退的比例分别为36.7%、32.6%、28.4%,相应激素替代为25.6%、84.3%、80.6%.单因素分析显示男性和肿瘤较大与腺垂体功能减退相关.结论 内分泌科就诊的垂体腺瘤术后患者半数存在腺垂体功能减退,而且有相当一部分功能减退的患者未能得到恰当的替代治疗.

  9. X-ray Lβ215 emission spectrum of Ru in Ru(NH3)6Cl3

    International Nuclear Information System (INIS)

    One of the broader applications of synchrotron radiation has been to EXAFS studies for material structure determination, i.e., for an analysis of x-ray absorption over an extended energy region beyond a core ionization limit. Studies of the near edge structure (XANES) give a different type of information, characteristic of the local symmetry and electronic configuration of the absorbing atom. This type of information is reflected also in the x-ray emission spectra, in particular for transitions involving the valence levels. Examination of the near edge absorption or the emission spectrum does not require an instrument capable of scanning a wide energy range with high counting statistics, as does EXAFS; the needs are rather for good resolution and a reliable calibration of the energy scale. Some of the problems of near edge spectra were particularly evident in our investigation of Ru-Lβ215 emission from Ru(NH3)6Cl3. The Ru-Lβ215 emission was measured with a laboratory Rowland circle x-ray spectrometer with a curved quartz (1010) crystal (radius = 22 inches) in a fixed position appropriate to the energy range, and a position sensitive detector which can be positioned along the Rowland circle. The Ru spectrum was excited mainly by Sn-L/sub α/ primary radiation from a Sn anode in a demountable x-ray tube operating at 13 kV and 120 mA. The resolution of the instrument in this region is 1.5 eV. An accurate calibration of the energy scale was conveniently obtained by measuring a reference x-ray emission line in the same instrumental configuration. In the present case the Pd-L/sub α/ emission line at 2838 eV was used to establish the energy scale. The energy dispersion of the instrument was determined from the Cl-K/sub β/ emission spectrum of CH3Cl between 2810 eV and 2830 eV and Pd-Lα12 and extrapolated to the energy region of the recorded emission spectrum. 6 references, 1 figure

  10. Characteristics of Distribution of 215 Acinetobacter Pneumonia and Drug Susceptibility Analysis in Respiratory Ward%215株致肺炎不动杆菌的分布特点与药敏分析

    Institute of Scientific and Technical Information of China (English)

    周煦; 李文朴; 李榜龙

    2009-01-01

    [Objective] To investigate the distribution of Acinetobacter pneumonia,drug susceptibility and its relationship with diseases of pneumonia. [Methods] All the Acinetobacter isolated from sputum from January 2000 to December 2007 were analyzed retrospectively. [Results] All 215 Acinetobacter isolates were collected, which contained 132 baumanii(61.4%), accounting to 14.3% of the total of sputum culture positive and 16.4% of negative bacilli. The number of Acinetobacter isolated from patients with lung underlying diseases was 177(82.3%). The result of drug susceptibility suggested that sensitive antibacterials were Cefoperazone/ sulbactam ,Meropenem,Cefepime and Levofloxacin, and resistant antibacterials were Aztreonam, Mezlocillin and Amphemycin. The drug susceptibility between 132 baumanii and 83 other Acinetobacter had significant difference(P<0.05). The results of Acinetobacter strain sensitivity whether or not isolated from the patients using respirator had significant differences(P<0.05). [Conclusion] Acinetobacter is the fundamental composition of lower respiratory tract bacterial infection,and Acinetobacter baumanii is the main. The drug susceptibility is obviously different between baumanii and other Acinetobacter. Acinetobacter is resistant to antibiotics widely and also related to respirator and lung underlying diseases. The characteristics of Acinetobacter infection is prone to patients with lung underlying diseases and is not only the main pathogenic bacteria of hospital infection,but also can cause the patients with lung underlying diseases to suffer from the community acquired pneumonia.%[目的]了解本院住院患者肺炎不动杆菌的分布、与疾病的关系及药物敏感情况.[方法]对本院2000年1月至2007年12月本院肺炎住院患者痰或支气管分泌物标本不动杆菌培养阳性结果结合病历进行回顾性统计分析.[结果]共分离出不动杆菌215株,占总分离菌株的14.3%(215/1503),革兰阴性杆菌的16.4%(215

  11. Isomers of small Pbn clusters (n=2-15) : Geometric and electronic structures based on ab initio molecular dynamics simulations

    Science.gov (United States)

    Rajesh, C.; Majumder, C.; Rajan, M. G. R.; Kulshreshtha, S. K.

    2005-12-01

    The geometric and electronic structure of the Pbn clusters (n=2-15) has been calculated to elucidate its structural evolution and compared with other group-IV elemental clusters. The search for several low-lying isomers was carried out using the ab initio molecular dynamics simulations under the framework of the density functional theory formalism. The results suggest that unlike Si, Ge, and Sn clusters, which favor less compact prolate shape in the small size range, Pb clusters favor compact spherical structures consisting of fivefold or sixfold symmetries. The difference in the growth motif can be attributed to their bulk crystal structure, which is diamond-like for Si, Ge, and Sn but fcc for Pb. The relative stability of Pbn clusters is analyzed based on the calculated binding energies and second difference in energy. The results suggest that n=4 , 7, 10, and 13 clusters are more stable than their respective neighbors, reflecting good agreement with experimental observation. Based on the fragmentation pattern it is seen that small clusters up to n=12 favor monomer evaporation, larger ones fragment into two stable daughter products. The experimental observation of large abundance for n=7 and lowest abundance of n=14 have been demonstrated from their fragmentation pattern. Finally a good agreement of our theoretical results with that of the experimental findings reported earlier implies accurate predictions of the ground state geometries of these clusters.

  12. Human Immunodeficiency Virus Type 1 Superinfection Was Not Detected following 215 Years of Injection Drug User Exposure

    Science.gov (United States)

    Tsui, Rose; Herring, Belinda L.; Barbour, Jason D.; Grant, Robert M.; Bacchetti, Peter; Kral, Alex; Edlin, Brian R.; Delwart, Eric L.

    2004-01-01

    Evidence for human immunodeficiency virus type 1 (HIV-1) superinfection was sought among 37 HIV-1-positive street-recruited active injection drug users (IDUs) from the San Francisco Bay area. HIV-1 sequences from pairs of samples collected 1 to 12 years apart, spanning a total of 215 years of exposure, were generated at p17 gag, the V3-V5 region of env, and/or the first exon of tat and phylogenetically analyzed. No evidence of HIV-1 superinfection was detected in which a highly divergent HIV-1 variant emerged at a frequency >20% of the serum viral quasispecies. Based on the reported risk behavior of the IDUs and the HIV-1 incidence in uninfected subjects in the same cohort, a total of 3.4 new infections would have been expected if existing infection conferred no protection from superinfection. Adjusted for risk behaviors, the estimated relative risk of superinfection compared with initial infection was therefore 0.0 (95% confidence interval, 0.00, 0.79; P = 0.02), indicating that existing infection conferred a statistically significant level of protection against superinfection with an HIV-1 strain of the same subtype, which was between 21 and 100%. PMID:14671091

  13. Words in melody: an H(2)15O PET study of brain activation during singing and speaking.

    Science.gov (United States)

    Jeffries, K J; Fritz, J B; Braun, A R

    2003-04-15

    We used H(2)15O PET to characterize the interaction of words and melody by comparing brain activity measured while subjects spoke or sang the words to a familiar song. Relative increases in activity during speaking vs singing were observed in the left hemisphere, in classical perisylvian language areas including the posterior superior temporal gyrus, supramarginal gyrus, and frontal operculum, as well as in Rolandic cortices and putamen. Relative increases in activity during singing were observed in the right hemisphere: these were maximal in the right anterior superior temporal gyrus and contiguous portions of the insula; relative increases associated with singing were also detected in the right anterior middle temporal gyrus and superior temporal sulcus, medial and dorsolateral prefrontal cortices, mesial temporal cortices and cerebellum, as well as in Rolandic cortices and nucleus accumbens. These results indicate that the production of words in song is associated with activation of regions within right hemisphere areas that are not mirror-image homologues of left hemisphere perisylvian language areas, and suggest that multiple neural networks may be involved in different aspects of singing. Right hemisphere mechanisms may support the fluency-evoking effects of singing in neurological disorders such as stuttering or aphasia.

  14. pHluorin enables insights into the transport mechanism of antiporter Mdr1: R215 is critical for drug/H+ antiport.

    Science.gov (United States)

    Redhu, Archana Kumari; Khandelwal, Nitesh Kumar; Banerjee, Atanu; Moreno, Alexis; Falson, Pierre; Prasad, Rajendra

    2016-10-01

    Multidrug resistance 1 (MDR1) is a member of the major facilitator superfamily that contributes to MDR of Candida albicans This antiporter belongs to the drug/H(+) antiporter 1 family, pairing the downhill gradient of protons to drug extrusion. Hence, drug efflux from cytosol to extracellular space and the parallel import of H(+) towards cytosol are inextricably linked processes. For monitoring the drug/H(+) antiporter activity of Mdr1p, we developed a new system, exploiting a GFP variant pHluorin, which changes its fluorescence properties with pH. This enabled us to measure the cytosolic pH correlated to drug efflux. Since protonation of charged residues is a key step in proton movement, we explored the role of all charged residues of the 12 transmembrane segments (TMSs) of Mdr1p in drug/H(+) transport by mutational analysis. This revealed that the conserved residue R(215), positioned close to the C-terminal end of TMS-4, is critical for drug/H(+) antiport, allowing protonation over a range of pH, in contrast with its H(215) or K(215) variants that failed to transport drugs at basic pH. Mutation of other residues of TMS-4 highlights the role of this TMS in drug transport, as confirmed by in silico modelling of Mdr1p and docking of drugs. The model points to the importance of R(215) in proton transport, suggesting that it may adopt two main conformations, one oriented towards the extracellular face and the other towards the centre of Mdr1p. Together, our results not only establish a new system for monitoring drug/H(+) transport, but also unveil a positively charged residue critical to Mdr1p function.

  15. The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Graham S.T.; Seymour, Lauren V. [Molecular and Cellular Biology, University of Guelph, Guelph, Ontario (Canada); Boggs, Joan M. [Molecular Structure and Function, Research Institute, Hospital for Sick Children, and Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario (Canada); Harauz, George, E-mail: gharauz@uoguelph.ca [Molecular and Cellular Biology, University of Guelph, Guelph, Ontario (Canada)

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer Full-length 21.5-kDa MBP isoform is translocated to the nucleus. Black-Right-Pointing-Pointer We hypothesized that the exon-II-encoded sequence contained the NLS. Black-Right-Pointing-Pointer We mutated this sequence in RFP-tagged constructs and transfected N19-cells. Black-Right-Pointing-Pointer Abolition of two key positively-charged residues resulted in loss of nuclear-trafficking. Black-Right-Pointing-Pointer The 21.5-kDa isoform of classic MBP contains a non-traditional PY-NLS. -- Abstract: The predominant 18.5-kDa classic myelin basic protein (MBP) is mainly responsible for compaction of the myelin sheath in the central nervous system, but is multifunctional, having numerous interactions with Ca{sup 2+}-calmodulin, actin, tubulin, and SH3-domains, and can tether these proteins to a lipid membrane in vitro. The full-length 21.5-kDa MBP isoform has an additional 26 residues encoded by exon-II of the classic gene, which causes it to be trafficked to the nucleus of oligodendrocytes (OLGs). We have performed site-directed mutagenesis of selected residues within this segment in red fluorescent protein (RFP)-tagged constructs, which were then transfected into the immortalized N19-OLG cell line to view protein localization using epifluorescence microscopy. We found that 21.5-kDa MBP contains two non-traditional PY-nuclear-localization signals, and that arginine and lysine residues within these motifs were involved in subcellular trafficking of this protein to the nucleus, where it may have functional roles during myelinogenesis.

  16. Production of actinium-225 for alpha particle mediated radioimmunotherapy.

    Science.gov (United States)

    Boll, Rose A; Malkemus, Dairin; Mirzadeh, Saed

    2005-05-01

    The initial clinical trials for treatment of acute myeloid leukemia have demonstrated the effectiveness of the alpha emitter (213)Bi in killing cancer cells. Bismuth-213 is obtained from a radionuclide generator system from decay of 10-days (225)Ac parent. Recent pre-clinical studies have also shown the potential application of both (213)Bi, and the (225)Ac parent radionuclide in a variety of cancer systems and targeted radiotherapy. This paper describes our five years of experience in production of (225)Ac in partial support of the on-going clinical trials. A four-step chemical process, consisting of both anion and cation exchange chromatography, is utilized for routine separation of carrier-free (225)Ac from a mixture of (228)Th, (229)Th and (232)Th. The separation of Ra and Ac from Th is achieved using the marcoporous anion exchange resin MP1 in 8M HNO(3) media. Two sequential MP1/NO(3) columns provide a separation factor of approximately 10(6) for Ra and Ac from Th. The separation of Ac from Ra is accomplished on a low cross-linking cation exchange resin AG50-X4 using 1.2M HNO(3) as eluant. Two sequential AG50/NO(3) columns provide a separation factor of approximately 10(2) for Ac from Ra. A 60-day processing schedule has been adopted in order to reduce the processing cost and to provide the highest levels of (225)Ac possible. Over an 8-week campaign, a total of approximately 100 mCi of (225)Ac (approximately 80% of the theoretical yield) is shipped in 5-6 batches, with the first batch typically consisting of approximately 50 mCi. After the initial separation and purification of Ac, the Ra pool is re-processed on a bi-weekly schedule or as needed to provide smaller batches of (225)Ac. The averaged radioisotopic purity of the (225)Ac was 99.6 +/- 0.7% with a (225)Ra content of < or =0.6%, and an average (229)Th content of (4(-4)(+5)) x 10(-5)%.

  17. Prediction of hsa-miR-21-5p Targeted Genes and It's Regulation Mechanisms in Acute Kidney Injury%hsa-miR-21-5p靶基因预测及其在急性肾损伤中的调控机制

    Institute of Scientific and Technical Information of China (English)

    邓旭; 李志辉

    2014-01-01

    目的:对hsa-miR-21-5p进行靶基因预测及生物信息学分析,为验证hsa-miR-21-5p靶基因及进一步研究hsa-miR-21-5p在急性肾损伤(AKI)中的调控作用提供数据支持.方法:应用Pubmed、Google等检索工具,以miR-21和AKI为关键词,检索miR-21与AKI相关的所有文献;应用miRBase获取各物种miR-21-5p序列,分析保守性;利用miRecord数据库进行靶基因预测,选取PicTar、PITA、RNAhybrid和TargetScan预测靶基因交集和DIANA LAB-TarBase 6.0已有实验数据支持的hsa-miR-21-5p靶基因作为研究的基因集合,进行功能注释、功能富集性分析(GO-analysis)及信号转导通路富集性分析.结果:miR-21水平在AKI肾脏、血及尿中均升高.miR-21-5p在多物种间具有序列保守性.hsa-miR-21-5p预测靶基因富集于生长发育、物质代谢与合成、细胞运动、细胞周期、增殖、凋亡、分化等生物学过程及蛋白连接、转录因子活性、转移酶活性、染色质结合、核苷酸结合、核苷结合、核酸结合、转录激活活性、连接酶活性等分子功能上,存在于胞内细胞器、细胞器界膜、细胞器组分、蛋白复合体、非膜性细胞器、细胞器管腔、极性生长位点等细胞组分中.预测靶基因富集于P53、TGF-β、细胞周期、MAPK、腺癌、膀胱癌、癌症通路、小细胞肺癌、慢性粒细胞白血病、黑色素瘤、前列腺癌、脑胶质瘤、大肠癌、非小细胞肺癌、子宫内膜癌、肌萎缩性侧索硬化症共16条通路中.结论:(1)miR-21水平在AKI肾脏、血及尿中均升高,对AKI有保护作用;(2)miR-21-5p在多物种间具有高度保守性;(3) hsa-miR-21-5p参与了多种生理、病理过程;(4)hsa-miR-21-5p对多条通路具有调控作用,可能通过调节细胞凋亡、炎症反应等参与AKI病理生理过程.

  18. Clinical outcome of 215 transmucosal implants with a conical connection: a retrospective study after 5-year follow-up.

    Science.gov (United States)

    Lopez, M A; Andreasi Bassi, M; Confalone, L; Gaudio, R M; Lombardo, L; Lauritano, D

    2016-01-01

    The purpose of this retrospective clinical study was to evaluate the survival rate (i.e. SVR – fixtures still in place at the end of the observation period) and success rate (i.e. SCR - bone resorption around implant neck) of an implant system characterized by cylindrical and tapered implants, both types of implant being equipped with a conical connection with an internal octagon (COC), both implant types having a 1.8 mm smooth neck, positioned above the bone crest level. A total of 65 subjects received 215 COCs between January 1996 and October 2011. All COCs were placed and restored by three experienced dental surgeons. The mean follow-up was 84±44 months. The patients involved in the study were both male (30) and female (35), of whom 30 were smokers (less than 20 cigarettes/day) and none was diabetic. The implants differed in terms of diameter and length, and were inserted both in the mandible (97) and in the maxilla (118). Sixty-seven implants were single tooth rehabilitations, and 148 prosthetic bridges. Fourteen had guided bone regeneration (GBR), and 10 were placed in post-extractive sites. Forty of the implants were provided with passing-screw abutments and 175 with full-screw abutments. The data were analyzed using descriptive statistics. None of the implants failed before prosthetic restoration, resulting in an SVR=100% after loading. The radiographic and clinical data revealed well-maintained, hard and soft tissue around the COCs, with an SCR=92.6%. Cox regression analyses did not detect any variables with statistical impact on the clinical outcome. In conclusion, Shiner XT implants are reliable tools for oral rehabilitation. PMID:27469549

  19. CFTR depletion results in changes in fatty acid composition and promotes lipogenesis in intestinal Caco 2/15 cells.

    Directory of Open Access Journals (Sweden)

    Geneviève Mailhot

    Full Text Available BACKGROUND: Abnormal fatty acid composition (FA in plasma and tissue lipids frequently occurs in homozygous and even in heterozygous carriers of cystic fibrosis transmembrane conductance regulator (CFTR mutations. The mechanism(s underlying these abnormalities remained, however, poorly understood despite the potentially CFTR contributing role. METHODOLOGY/PRINCIPAL FINDINGS: The aim of the present study was to investigate the impact of CFTR depletion on FA uptake, composition and metabolism using the intestinal Caco-2/15 cell line. shRNA-mediated cftr gene silencing induced qualitative and quantitative modifications in FA composition in differentiated enterocytes as determined by gas-liquid chromatography. With the cftr gene disruption, there was a 1,5 fold increase in the total FA amount, largely attributable to monounsaturated and saturated FA compared to controls. The activity of delta-7 desaturase, estimated by the 16:1(n-7/16:0, was significantly higher in knockdown cells and consistent with the striking elevation of the n-7 FA family. When incubated with [14C]-oleic acid, CFTR-depleted cells were capable of quick incorporation and export to the medium concomitantly with the high protein expression of L-FABP known to promote intracellular FA trafficking. Accordingly, lipoprotein vehicles (CM, VLDL, LDL and HDL, isolated from CFTR knockdown cells, exhibited higher levels of radiolabeled FA. Moreover, in the presence of [14C]-acetate, knockdown cells exhibited enhanced secretion of newly synthesized phospholipids, triglycerides, cholesteryl esters and free FA, thereby suggesting a stimulation of the lipogenic pathway. Conformably, gene expression of SREBP-1c, a key lipogenic transcription factor, was increased while protein expression of the phosphorylated and inactive form of acetylCoA carboxylase was reduced, confirming lipogenesis induction. Finally, CFTR-depleted cells exhibited lower gene expression of transcription factors (PPARalpha

  20. [Characteristics and influence of different clinical forms on the development and prognosis of carotid sinus syndrome. Apropos of 215 cases].

    Science.gov (United States)

    Graux, P; Carlioz, R; Guyomar, Y; Lemaire, N; Rihani, R; Cornaert, P; Dutoit, A; Croccel, L

    1995-07-01

    The carotid sinus syndrome is a well-known cause of syncope: the cardio-inhibitory forms are the easiest to diagnose and probably the easiest to treat. However, the vasodepressive forms are as common but their outcome is mainly unknown. Eight hundred and fifty-three patients underwent endocavitary electrophysiological studies with invasive blood pressure measurement for unexplained syncope between October 1984 and January 1990. A carotid sinus syndrome was diagnosed in 215 cases. Fifty-two patients (24.2%) had a cardio-inhibitory form (ventricular standstill > or = 3 s during carotid sinus massage), 40 (18.6%) had a pure vasodepressive form (isolated fall of systolic blood pressure > 50 mmHg during massage) and 123 patients (57.2%) had a mixed form. The average age was 74.1 +/- 9.7 years with no difference between the different forms. A number of parameters was different on the cardio-inhibitory and vasodepressive forms: the number of men (75.6 vas 24.4%; p < 0.05) and the number of syncopes (83.3 vs 60%; p < 0.02) were greater in the cardio-inhibitory form; the vasodepressive forms were more often associated with a history of transient ischaemic attacks (15 vs 0%), a poor cardiovascular status (hypertension: 47.5 vs 15.7%; p < 0.01), coronary artery disease (47.5 vs 25.5%; p < 0.05), cardiac failure (27.5 vs 11.7%; p < 0.05), induction of sustained supraventricular tachycardia (50 vs 23.5%; p < 0.05) and a greater pacemaker effect (53.6 vs 34.8 mmHg; p < 0.01); of the 191 patients (84.9% of the population) followed up for an average of 21.2 months, 168 received treatment: implantation of a cardiac pacemaker in 108 patients, reduction of antihypertensive and/or potentially bradycardia-inducing drugs in 30 patients, prescription of antiarrhythmic therapy, in 30 patients.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7487332

  1. Non-motor symptoms in a Flanders-Belgian population of 215 Parkinson’s disease patients as assessed by the Non-Motor Symptoms Questionnaire

    OpenAIRE

    Crosiers, David; Pickut, Barbara; Theuns, Jessie; De Deyn, Peter Paul; Van Broeckhoven, Christine; Martinez-Martin, Pablo; Chaudhuri, K. Ray; Cras, Patrick

    2012-01-01

    Background: Non-motor symptoms (NMS) in Parkinson’s disease (PD) are frequent, increase the patients’ disability and have an important negative impact on their quality of life. Methods: We used a Dutch translation of the Non-Motor Symptoms Questionnaire (NMSQuest) to identify NMS in a population of 215 Belgian PD patients. Results: A median number of 8 NMS was reported per patient. Urinary urgency (59.2 %), nocturia (56.9 %), insomnia (45.8 %), attention problems (45.5 %) and orthostatism (41...

  2. Investigation of 70SiO2-15CaO-10P2O5-5Na2O Glass Composition for Bone Regeneration Applications

    OpenAIRE

    Vikas Anand; Kanwar J. Singh; Kulwinder Kaur; Daljit Singh Arora; Harpreet Kaur

    2014-01-01

    Glass with the composition 70SiO2-15CaO -10P2O5-5Na2O has been prepared by using sol gel technique. Bioactive behavior of the glass sample has been checked by in vitro study using TRIS simulated body fluid. Bioactive properties of the sample has been analyzed by using XRD, Raman, FE-SEM, EDX and Brunauer Emmett Teller studies. pH study has been conducted to check the non- acidic nature of the glass sample. Drug delivery behavior of the sample has been estimated by using gentamicin as an antib...

  3. Impactos de atropelamentos de animais silvestres no trecho da rodovia SP-215 confrontante ao Parque Estadual de Porto Ferreira – Porto Ferreira, SP (Nota Científica. Impacts roadkills mortality of wild animals in the area of SP-215 highway alongside Porto Ferreira State Park – Porto Ferreira, SP (Scientific Note.

    Directory of Open Access Journals (Sweden)

    Sonia Aparecida de SOUZA

    2010-12-01

    Full Text Available No presente trabalho foi realizado o monitoramento dos atropelamentos de animais silvestres na rodovia SP–215 no trecho confrontante ao Parque Estadual de Porto Ferreira. Em seis anos foram registrados 72 indivíduos, sendo 17 espécies de mamíferos, 10 de aves, quatro de répteis, uma de anfíbio e três indivíduos não identificados. Dentre esses, lobo-guará Chrysocyon brachyurus, jaguatirica Leopardus pardalis e cuíca-lanosa Caluromys lanatus se encontram na lista das espécies ameaçadas de extinção do Estado de São Paulo. A partir do conhecimento das espécies atingidas, faz-se necessário o desenvolvimento de estudos e propostas de implantação de medidas mitigadoras à presença da rodovia SP–215 para a conservação da fauna.This paper presents the data collected through the monitoring of roadkills on SP–215 highway in the area alongside Porto Ferreira State Park. During six years, 72 roadkills were recorded, including 17 species of mammals, 10 of birds, five of reptiles, one of amphibians and three unidentified individuals. Among those species, maned wolf Chrysocyon brachyurus, ocelot Leopardus pardalis, and Western woolly opossum Caluromys lanatus are included in the threatened species list of the state of São Paulo. After finding out which species are affected, it is necessary to develop studies and proposals for implementing effective mitigation measures for the presence of SP–215 highway in the area in order to preserve the local fauna.

  4. 应用Surrogate报告载体提高CRISPR/Cas9对TMEM215基因的打靶效率%Improving Gene Targeting Efficiency on TMEM215 Mediated by CRISPR/Cas9 by Using Surrogate Reporter

    Institute of Scientific and Technical Information of China (English)

    高晓彤; 付伟; 陈娟娟; 胡彬; 李丹慧; 黄斯勇; 李国辉; 张阳萍; 刘利

    2016-01-01

    目的:构建Surrogate报告载体,并利用Surrogate报告载体提高CRISPR/Cas9对HEK293T细胞TMEM215基因打靶效率.方法:构建针对人TMEM215的CRISPR/Cas9表达载体及相应Surrogate报告载体,两者共转HEK293T细胞,通过流式分析、T7EI检测、TA克隆测序等明确Surrogate报告载体对不同sgRNA打靶效率的检测及对基因修饰细胞的筛选富集作用.结果:流式分析结果表明,Surrogate报告载体成功检测出不同sgRNA的打靶效率,并筛选出高效率sgRNA;T7EI检测及TA克隆测序显示,外加嘌呤霉素抗性筛选时,Surrogate报告载体可有效富集基因修饰细胞.结论:成功构建Surrogate报告载体,并利用Surrogate报告载体提高CRISPR/Cas9对HEK293T细胞TME M215基因的打靶效率.

  5. Phase separation of myelin sheath in Triton X-114 solution: predominant localization of the 21.5-kDa isoform of myelin basic protein in the lipid raft-associated domain.

    Science.gov (United States)

    Uruse, Michihiro; Yamamoto, Masahiro; Sugawa, Makoto; Matsuura, Keiko; Sato, Yurie; Seiwa, Chika; Watanabe, Kenji; Aiso, Sadakazu; Asou, Hiroaki

    2014-04-01

    Myelin basic protein (MBP) isoforms in the myelin sheath are known to have distinct intracellular expression patterns, which are profoundly related to functional specificity. Determining the differential localization of MBP isoforms is therefore important for understanding their pathophysiological roles. In this study, we have developed a new method for phase separation of myelin. The non-ionic detergent Triton X-114 is used to solubilize myelin sheath which then undergoes phase separation to yield four fractions. The lipid raft-associated proteins and lipids in each fraction were analysed by immunoblotting and lipid analysis, respectively. The present method gives two lipid raft-enriched fractions, one of them was found to contain only lipid raft-associated galactocerebroside and cholesterol as the major lipids. The 21.5-kDa MBP isoforms (21.5 MBP), both unphosphorylated and phosphorylated, were exclusively contained in this fraction. Phosphorylated 21.5 MBP (21.5 pMBP) has been shown to specifically disappear from demyelinated loci. The present analytical method clearly indicated that disappearance of 21.5 pMBP corresponded to demyelination and its reappearance corresponded to prevention of demyelination. Demyelination was also associated with aging and was prevented by the myelin-protecting herbal medicine, Chinpi, a type of dried citrus peel.

  6. Myelin management by the 18.5-kDa and 21.5-kDa classic myelin basic protein isoforms.

    Science.gov (United States)

    Harauz, George; Boggs, Joan M

    2013-05-01

    The classic myelin basic protein (MBP) splice isoforms range in nominal molecular mass from 14 to 21.5 kDa, and arise from the gene in the oligodendrocyte lineage (Golli) in maturing oligodendrocytes. The 18.5-kDa isoform that predominates in adult myelin adheres the cytosolic surfaces of oligodendrocyte membranes together, and forms a two-dimensional molecular sieve restricting protein diffusion into compact myelin. However, this protein has additional roles including cytoskeletal assembly and membrane extension, binding to SH3-domains, participation in Fyn-mediated signaling pathways, sequestration of phosphoinositides, and maintenance of calcium homeostasis. Of the diverse post-translational modifications of this isoform, phosphorylation is the most dynamic, and modulates 18.5-kDa MBP's protein-membrane and protein-protein interactions, indicative of a rich repertoire of functions. In developing and mature myelin, phosphorylation can result in microdomain or even nuclear targeting of the protein, supporting the conclusion that 18.5-kDa MBP has significant roles beyond membrane adhesion. The full-length, early-developmental 21.5-kDa splice isoform is predominantly karyophilic due to a non-traditional P-Y nuclear localization signal, with effects such as promotion of oligodendrocyte proliferation. We discuss in vitro and recent in vivo evidence for multifunctionality of these classic basic proteins of myelin, and argue for a systematic evaluation of the temporal and spatial distributions of these protein isoforms, and their modified variants, during oligodendrocyte differentiation.

  7. Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.

    Science.gov (United States)

    Mahyous Saeyd, Salah Adam; Ewert-Krzemieniewska, Katarzyna; Liu, Boyin; Caspari, Thomas

    2014-07-01

    Although it is well established that Cdc2 kinase phosphorylates the DNA damage checkpoint protein Crb2(53BP1) in mitosis, the full impact of this modification is still unclear. The Tudor-BRCT domain protein Crb2 binds to modified histones at DNA lesions to mediate the activation of Chk1 by Rad3ATR kinase. We demonstrate here that fission yeast cells harbouring a hyperactive Cdc2CDK1 mutation (cdc2.1w) are specifically sensitive to the topoisomerase 1 inhibitor camptothecin (CPT) which breaks DNA replication forks. Unlike wild-type cells, which delay only briefly in CPT medium by activating Chk1 kinase, cdc2.1w cells bypass Chk1 to enter an extended cell-cycle arrest which depends on Cds1 kinase. Intriguingly, the ability to bypass Chk1 requires the mitotic Cdc2 phosphorylation site Crb2-T215. This implies that the presence of the mitotic phosphorylation at Crb2-T215 channels Rad3 activity towards Cds1 instead of Chk1 when forks break in S phase. We also provide evidence that hyperactive Cdc2.1w locks cells in a G1-like DNA repair mode which favours non-homologous end joining over interchromosomal recombination. Taken together, our data support a model such that elevated Cdc2 activity delays the transition of Crb2 from its G1 to its G2 mode by blocking Srs2 DNA helicase and Casein Kinase 1 (Hhp1).

  8. Reliability study on the preparation of red cell products by ACP-215 automatic red cell treatment instrument%ACP-215全自动红细胞处理仪制备红细胞制品的可靠性研究

    Institute of Scientific and Technical Information of China (English)

    刘军; 张树重; 杜振军

    2013-01-01

    Objective:To study the reliability of preparing red cell products by ACP-215 automatic red cell treatment instrument. Methods:The subjects were divided into two groups, the control group using manual open washing preparation, the experiment group adopted the ACP-215 automated red cell treatment instrument preparation. Deglycerin erythrocyte and washed red blood cells in accordance with the relevant standards, recovery rate of RBC, hemoglobin, glycerol content, cultivation of residual white blood cells, in vitro hemolysis test and bacteria cultivation were detected and analyzed in the experimental group and the control group. Results:To deglycerin erythrocyte, the experimental group in the recovery rate of RBC, WBC, cultivation of residual glycerol content, in vitro hemolysis and bacteria cultivation was better than the control group;for the washed red blood cells, the experimental group in the recovery rate of RBC, residual white blood cells and supernatant protein content was better than the control group. Conclusions:Using ACP-215 automatic red blood cell processing apparatus for preparation of a red blood cell products, it was more safe and reliable than the hand washing method, especially in deglycerin erythrocyte, The instrument has the practical application value and was worthy of promotion.%目的:研究ACP-215全自动红细胞处理仪制备红细胞制品的可靠性。方法:将研究对象分成两组,对照组采用手工开放式洗涤法制备,试验组采用ACP-215全自动红细胞处理仪制备。解冻去甘油红细胞和洗涤红细胞按照相关标准,分别对实验组和对照组红细胞回收率、游离血红蛋白、甘油含量、残留白细胞、体外溶血试验和细菌培养进行检测分析。结果:对于解冻去甘油红细胞,实验组在红细胞回收率、残留白细胞、甘油含量、体外溶血和细菌培养均好于对照组;对于洗涤红细胞,实验组在红细胞回收率、残留白细

  9. Characterization of osteoclasts from patients harboring a G215R mutation in ClC-7 causing autosomal dominant osteopetrosis type II

    DEFF Research Database (Denmark)

    Henriksen, Kim; Gram, Jeppe; Schaller, Sophie;

    2004-01-01

    from ADOII patients and healthy age- and sex-matched controls, were used to evaluate osteoclastogenesis, cell fusion, acidification, and resorptive activity. ADOII osteoclasts in vivo have increased number and size. However, in vitro we observed no significant changes in the osteoclast formation rate......Autosomal dominant osteopetrosis II (ADOII) is a relatively benign disorder caused by a missense mutation in the ClCN7 gene. In this study, we characterize the osteoclasts from patients with ADOII, caused by a G215R mutation, and investigate the effect on osteoclast function in vitro. Osteoclasts......, the morphology, and the expression of markers, such as cathepsin K and tartrate-resistant acid phosphatase. When mature ADOII osteoclasts were investigated on mineralized bone, they degraded the bone material, however only to 10 to 20% of the level in controls. We show by acridine orange, that the reduced...

  10. Investigation of 70SiO2-15CaO-10P2O5-5Na2O Glass Composition for Bone Regeneration Applications

    Directory of Open Access Journals (Sweden)

    Vikas Anand

    2014-11-01

    Full Text Available Glass with the composition 70SiO2-15CaO -10P2O5-5Na2O has been prepared by using sol gel technique. Bioactive behavior of the glass sample has been checked by in vitro study using TRIS simulated body fluid. Bioactive properties of the sample has been analyzed by using XRD, Raman, FE-SEM, EDX and Brunauer Emmett Teller studies. pH study has been conducted to check the non- acidic nature of the glass sample. Drug delivery behavior of the sample has been estimated by using gentamicin as an antibiotic. Reported sample has been found to be potential candidate for bone regeneration applications.

  11. Up- and down-conversion luminescence in the oxyfluoride glass ceramics containing Ba2+1.5xYb1−xF7:Tb3+ nanocrystals

    International Nuclear Information System (INIS)

    Transparent oxyfluoride borosilicate glass ceramics containing Ba2+1.5xYb1−xF7:Tb3+ nanocrystals were successfully prepared by a melt-quenching method with subsequent heat treatment. The precipitated crystalline phase in the glass matrix changed gradually from BaF2 to Ba2+1.5xYb1−xF7 with the increase of YbF3 content, which was confirmed by the results of XRD, HRTEM and EDX measurements. The ultraviolet and visible up-conversion and near-infrared quantum cutting down-conversion emissions were observed and interpreted. These materials could be used to modify the solar spectrum and enhance the silicon solar cell efficiency by the up-conversion and down-conversion luminescence of Tb3+–Yb3+ couples in the oxyfluoride borosilicate glass ceramics. -- Highlights: ► Oxyfluoride glass ceramics containing Ba2+1.5xYb1−xF7:Tb nanocrystals were prepared. ► The precipitated crystal phase changed from BaF2 to Ba2+1.5xYb1−xF7. ► Strong up-conversion and down-conversion emissions were observed and interpreted.

  12. Up- and down-conversion luminescence in the oxyfluoride glass ceramics containing Ba{sub 2+1.5x}Yb{sub 1-x}F{sub 7}:Tb{sup 3+} nanocrystals

    Energy Technology Data Exchange (ETDEWEB)

    Xin, Fengxia [College of Materials Science and Engineering, China Jiliang University, Hangzhou 310018 (China); Zhao, Shilong, E-mail: shilong_zhao@hotmail.com [College of Materials Science and Engineering, China Jiliang University, Hangzhou 310018 (China); Jia, Guohua; Huang, Lihui; Deng, Degang; Wang, Huanping [College of Materials Science and Engineering, China Jiliang University, Hangzhou 310018 (China); Xu, Shiqing, E-mail: sxucjlu@hotmail.com [College of Materials Science and Engineering, China Jiliang University, Hangzhou 310018 (China)

    2012-11-15

    Transparent oxyfluoride borosilicate glass ceramics containing Ba{sub 2+1.5x}Yb{sub 1-x}F{sub 7}:Tb{sup 3+} nanocrystals were successfully prepared by a melt-quenching method with subsequent heat treatment. The precipitated crystalline phase in the glass matrix changed gradually from BaF{sub 2} to Ba{sub 2+1.5x}Yb{sub 1-x}F{sub 7} with the increase of YbF{sub 3} content, which was confirmed by the results of XRD, HRTEM and EDX measurements. The ultraviolet and visible up-conversion and near-infrared quantum cutting down-conversion emissions were observed and interpreted. These materials could be used to modify the solar spectrum and enhance the silicon solar cell efficiency by the up-conversion and down-conversion luminescence of Tb{sup 3+}-Yb{sup 3+} couples in the oxyfluoride borosilicate glass ceramics. -- Highlights: Black-Right-Pointing-Pointer Oxyfluoride glass ceramics containing Ba{sub 2+1.5x}Yb{sub 1-x}F{sub 7}:Tb nanocrystals were prepared. Black-Right-Pointing-Pointer The precipitated crystal phase changed from BaF{sub 2} to Ba{sub 2+1.5x}Yb{sub 1-x}F{sub 7}. Black-Right-Pointing-Pointer Strong up-conversion and down-conversion emissions were observed and interpreted.

  13. Microbiological transformations. Part 48 : Enantioselective biohydrolysis of 2-, 3- and 4-pyridyloxirane at high substrate concentration using the Agrobacterium radiobacter AD1 epoxide hydrolase and its Tyr215Phe mutant

    NARCIS (Netherlands)

    Genzel, Y; Archelas, A; Lutje Spelberg, Jeffrey H.; Janssen, DB; Furstoss, R

    2001-01-01

    The epoxide hydrolase (EH) from Agrobacterium radiobacter AD1 wild type (ArWT) and its Tyr215Phe mutant were compared for the biocatalyzed hydrolytic kinetic resolution (BHKR) of 2-, 3- and 4-pyridyloxirane. The radioselectivity of the oxirane ring opening as well as the substrate concentration limi

  14. Rate of tumor sterilisation of lung cancer by external beam irradiation; Lokale Effizienz der perkutanen Radiotherapie beim Lungenkrebs. Analyse von 215 Rebronchoskopien in Abhaengigkeit von der applizierten Bestrahlungsdosis

    Energy Technology Data Exchange (ETDEWEB)

    Schwegler, N. [Abt. Strahlentherapie, Kantonsspital, Aarau (Switzerland); Vrh, T. [Abt. Strahlentherapie, Kantonsspital, Aarau (Switzerland); Kern, T. [Abt. Strahlentherapie, Kantonsspital, Aarau (Switzerland); Notter, M. [Abt. Strahlentherapie, Kantonsspital, Aarau (Switzerland); Frey, M. [Abt. Pneumologie, Klinik Barmelweid (Switzerland); Grossenbacher, M. [Abt. Pneumologie, Kantonsspital, Aarau (Switzerland); Hausmann, M. [Abt. Pneumologie, Klinik Barmelweid (Switzerland); Pfenninger, T. [Abt. Pneumologie, Kantonsspital, Olten (Switzerland); Ragaz, A. [Abt. Pneumologie, Kantonsspital, Aarau (Switzerland); Schmid, R. [Abt. Innere Medizin, Regionalspital, Muri (Switzerland); Siebenschein, R. [Abt. Pneumologie, Kantonsspital, Baden (Switzerland); Keller, R. [Abt. Pneumologie, Klinik Barmelweid (Switzerland)

    1996-02-01

    The prospective, together with the pneumologists, defined treatment concept included the repetition of bronchoscopic evaluations after the application of 60 Gy and 80 Gy. These radiation doses from 60 Gy up to 80 Gy have been given with a shrinking-field technique to the mediastinum and the primary. In order to record statistically the optical tumor changements we were urged to create a so-called bT-score. The structure of this score was orientated towards the periphery of the tracheobronchial tree. Hundred and forty-four patients with endoscopically and histologically verified bronchogenic carcinomas were treated. On the subjects 215 re-bronchoscopies accomplished with biopsies were performed and allowed to analyze the macro- and microscopical behavior under treatment. A histological/cytological elimination of tumor was achieved after 60 Gy in 35.1%, after 80 Gy in 62.3%. Macroscopically no tumor was visible after 60 Gy in 43.6%, after 80 Gy in 82%. A correlation between identical micro- and macroscopical observations was only seen in 61%, respectively in 71%. The escalation of the radiation dose from 60 Gy up to 80 Gy with shrinked fields could increase the local tumor sterilization rate by 1.8 times from 35.1% to 62.3%. (orig./MG) [Deutsch] Im Rahmen eines mit den Pneumonologen prospektiv festgelegten Behandlungsschemas applizierten wir 60 Gy bis 80 Gy unter progredienter Feldverkleinerung auf Primaertumor und Mediastinum und strebten endoskopische und histologische Untersuchungen nach 60 Gy und 80 Gy an. Um die optisch festgestellten Tumorveraenderungen statistisch erfassen zu koennen, waren wir gezwungen, einen sogenannten bT-Score zu schaffen, dessen Gliederung sich an die Verjuengung des Tracheobronchialbaumes nach peripher orientierte. An 144 endoskopisch und histologisch gesicherten Bronchuskarzinomen wurden 215 Rebronchoskopien - ergaenzt durch Gewebsentnahmen - durchgefuehrt. Die ausgewerteten Resultate gestatteten, das makroskopische und mikroskopische

  15. Atomic and electronic structures of neutral and charged Pbn clusters (n=2-15): Theoretical investigation based on density functional theory

    Science.gov (United States)

    Rajesh, Chinagandham; Majumder, Chiranjib

    2007-06-01

    The geometric and electronic structures of the Pbn+ clusters (n=2-15) have been investigated and compared with neutral clusters. The search for several low-lying isomers was carried out under the framework of the density functional theory formalism using the generalized gradient approximation for the exchange correlation energy. The wave functions were expanded using a plane wave basis set and the electron-ion interactions have been described by the projector augmented wave method. The ground state geometries of the singly positively charged Pbn+ clusters showed compact growth pattern as those observed for neutrals with small local distortions. Based on the total energy of the lowest energy isomers, a systematic analysis was carried out to obtain the physicochemical properties, viz., binding energy, second order difference in energy, and fragmentation behavior. It is found that n =4, 7, 10, and 13 clusters are more stable than their neighbors, reflecting good agreement with experimental observation. The chemical stability of these clusters was analyzed by evaluating their energy gap between the highest occupied and lowest unoccupied molecular orbitals and adiabatic ionization potentials. The results revealed that, although Pb13 showed higher stability from the total energy analysis, its energy gap and ionization potential do not follow the trend. Albeit of higher stability in terms of binding energy, the lower ionization potential of Pb13 is interesting which has been explained based on its electronic structure through the density of states and electron shell filling model of spherical clusters.

  16. Exploring the dust content of SDSS DR7 damped Lyman alpha systems at 2.15$\\le z_{ab}< $5.2

    CERN Document Server

    Khare, Pushpa; York, Donald G; Lundgren, Britt; Kulkarni, Varsha P

    2011-01-01

    We have studied a sample of 1084 intervening absorption systems with 2.15$\\le z_{ab}\\le$5.2, having log(N$_{\\rm HI}$) $>$ 20.0 in the spectra of QSOs in Sloan Digital Sky Survey (SDSS) data release 7 (DR7), with the aim of understanding the nature and abundance of the dust and the chemical abundances in the DLA absorbers. Composite spectra were constructed for the full sample and several subsamples, chosen on the basis of absorber and QSO properties. Average extinction curves were obtained for the samples by comparing their geometric mean composite spectra with those of two samples of QSOs, matching in z$_{em}$ and i magnitude with the DLA sample, one sample without any absorbers along their lines of sight and the other without any DLAs along their lines of sight irrespective of the presence of other absorption systems. While the average reddening in the DLA sample is small, we find definite evidence for the presence of dust in subsamples based on absorber properties, in particular the strength of metal absor...

  17. SPECTRUM OF CERVICAL LESIONS IN CIMS, BILASPUR: A 5 YEAR RETROSPECTIVE STUDY OF 215 CASES IN A TERTIARY HOSPITAL OF CENTRAL INDIA

    Directory of Open Access Journals (Sweden)

    Sadhna

    2015-10-01

    Full Text Available BACKGROUND AND OBJEC TIVE: Cancer of the cervix is a leading cause of morbidity and mortality among women W orld W ide. Therefore to curb the disease there is need of awareness of this disease. This study is aimed to categorize the premalignant and malignant lesions at the earliest, to reduce the mortality and morbidity. It also aimed to know the incidence of malignancies at our set up and to calculate distribution of patient in relation to parity and symptoms. DESIGN AND SETTING: Study included 215 patients with complain of white dis charge per vagina, per vaginal bleeding and backache attending the gynecology OPD over a period of 05 years. PATIENT AND METHODS: Patients were subjected to cervical biopsy and biopsies were sent to pathology department. Detailed clinical history, age, age at marriage, parity and socio - economic status was obtained. RESULT: Result were classified histopathological l y as inflammatory lesions 70 cases, polypoidal lesion were 30, LSIL accounted for 32 cases and HSIL were 20 cases and 30 were malignant lesions. U nder malignant category moderately differentiated squamous cell carcinoma was the commonest. Maximum numbers of patients were subjected to cervical biopsies in 3 rd decade of life. Malignancies were common in 3 rd & 4 th decade of life. Most of the cases were of parity 4. CONCLUSION : Tissue biopsy is a valuable diagnostic procedure on which surgeons and radiologists still rely.

  18. Wavelength stabilized 785 nm DBR-ridge waveguide lasers with an output power of up to 215 mW

    International Nuclear Information System (INIS)

    Wavelength stabilized distributed Bragg reflector (DBR) diode lasers at an emission wavelength of 785 nm will be presented. The devices have a 14 nm thick GaAsP single quantum well as active layer, which is embedded in Al0.65Ga0.35As waveguide layers and Al0.7Ga0.3As cladding layers. The DBR structures are realized as deeply etched tenth order gratings using I-line wafer stepper lithography. The devices have a stripe width of 2.2 µm and a cavity length of 3 mm including a DBR grating with a length of 500 µm. The devices are mounted p-side up on C-mounts. At a temperature of 25 °C a continuous wave output power of 215 mW and a conversion efficiency of 28% are measured. Up to 140 mW single mode operation with a small tuning range below 190 pm is observed. At 50 mW an aging test was performed showing reliable operation over 1000 h. (paper)

  19. 铁磁形状记忆合金Ni50Mn28.5Ga21.5单晶变体强磁场处理及磁致应变%HIGH MAGNETIC FIELD TREATMENT OF VARIANTS IN FERROMAGNETIC SHAPE MEMORY ALLOY Ni50Mn28.5Ga21.5 SINGLE CRYSTAL AND MAGNETOSTRAIN

    Institute of Scientific and Technical Information of China (English)

    李灼; 王敬民; 刘伟超; 蒋成保

    2008-01-01

    采用光子加热悬浮区熔法制备出直径7 mm、长60 mm的Ni50Mn28.5Ga21.5单晶.分别切割成8.1 mm×4.3mm×4.4 mm和直径7mm,长38.9 mm的两块单晶体,利用10 T强磁场进行变体处理获得近似单变体,并获得了5.2%的大磁致应变.磁致应变压力效应的测试表明,随压应力增大,孪晶再取向的临界磁场强度增大,磁致应变降低.

  20. 长脉冲1064nm Nd:YAG激光治疗婴幼儿皮肤血管瘤215例疗效分析%A clinical effect analysis of long-pulsed 1064 nm Nd:YAG laser in the treatment of 215 infancy hemangiomas

    Institute of Scientific and Technical Information of China (English)

    曹丽华; 张美华; 吕东; 秦萍萍; 周洋

    2012-01-01

    Objectives: To observe the clinical effects of long-pulsed 1 064 nm Nd:YAG laser in the treatment of heman-giomas of skin in infant and investigate its related factors. Methods: Total 215 cases were treated with long-pulsed 1 064 nm Nd:YAG laser according to different disease types, including 19 cases of port wine stains, 179 cases of strawberry heman-giomas and 17 cases of mixed hemangiomas. The association between clinical effect and types, size, location and times of treatment of hemangiomas was also analyzed. Results: Among 215 cases, 133 were cured, 67 gained marked effect, 8 gaining effective therapy and 7 ineffective. The cure rate and total efficacy rate were 61.9% and 93.1%, respectively. The effect was related with the type, size and treatment times of hemangiomas. However, the location of hemangiomas was not associated with the therapy effect. The side-effect rate was 48.8% with temporary hyper-pigmentation, 8.3% with temporary hypo-pigmentation, 4.6% with superficial scar and 3.7% with hair reduction or disappearance. Conclusion: The long-pulsed 1 064nm Nd:YAG laser is an effective, safe and low side-effect treatment for infancy hemangiomas.%目的:观察长脉冲1064nm Nd:YAG激光治疗婴幼儿皮肤血管瘤临床疗效,并探讨影响疗效的相关因素.方法:应用长脉冲1 064 nm Nd:YAG激光治疗婴幼儿皮肤血管瘤215例,其中鲜红斑痣19例,草莓状血管瘤179例,混合型血管瘤17例.分析其疗效与病变类型、瘤体大小、病变部位及治疗次数的关系.结果:215例患儿中治愈133例,显效67例,有效8例,无效7例,痊愈率和有效率分别为61.9%和93.1%.疗效与血管瘤的病变类型、瘤体的大小以及治疗次数有关,但与其病变部位无明显相关性.治疗后暂时性色素沉着、暂时性色素减退、浅表性瘢痕、头部毛发减少或消失的发生率分别为48.8%、8.3%、4.6%和3.7%.结论:长脉冲1064nm Nd:YAG激光治疗婴幼儿皮肤血管瘤疗效好,

  1. Surgical skill experience of 215 cases of pterygium treated by autologous corneal limbal stem cell transplantation%215例自体角膜缘干细胞移植治疗翼状胬肉手术技巧体会

    Institute of Scientific and Technical Information of China (English)

    潘勇; 葛军

    2014-01-01

    目的:探讨自体角膜缘干细胞移植治疗翼状胬肉手术技巧。方法回顾我院2006年8月至2013年8月采用自体角膜缘干细胞移植治疗翼状胬肉手术215例(267眼),对手术效果进行临床分析。结果术后随访1~3年,212例(264眼)治愈无复发,占98.9%,3例(3眼)复发,占1.1%。结论自体角膜缘干细胞移植治疗翼状胬肉手术疗效显著,复发率低,非常适合在各级医院推广。%Objective To investigate the surgical skill of autologous corneal limbal stem cell transplantation for the treatment of pterygium. Methods The medical records of 267 eyes of 215 patients who underwent autologous corneal limbal stem cell transplantation for pterygium in our hospital during Aug 2006 and Aug 2013 was reviewed. A clinical analysis was made on the effect of the treatment. Results Patients were followed up for 1-3 years. 264 eyes of 212 patients (98.9%) were cured without recurrence, while only 3 eyes of 3 patients (1.1%) recurred. Conclusion Autologous corneal limbal stem cell transplantation is an effective treatment for pterygium and has a low recurrence rate. It is very suitable for being popularized in varied levels of hospitals.

  2. Corrections of arterial input function for dynamic H215O PET to assess perfusion of pelvic tumours: arterial blood sampling versus image extraction

    Science.gov (United States)

    Lüdemann, L.; Sreenivasa, G.; Michel, R.; Rosner, C.; Plotkin, M.; Felix, R.; Wust, P.; Amthauer, H.

    2006-06-01

    Assessment of perfusion with 15O-labelled water (H215O) requires measurement of the arterial input function (AIF). The arterial time activity curve (TAC) measured using the peripheral sampling scheme requires corrections for delay and dispersion. In this study, parametrizations with and without arterial spillover correction for fitting of the tissue curve are evaluated. Additionally, a completely noninvasive method for generation of the AIF from a dynamic positron emission tomography (PET) acquisition is applied to assess perfusion of pelvic tumours. This method uses a volume of interest (VOI) to extract the TAC from the femoral artery. The VOI TAC is corrected for spillover using a separate tissue TAC and for recovery by determining the recovery coefficient on a coregistered CT data set. The techniques were applied in five patients with pelvic tumours who underwent a total of 11 examinations. Delay and dispersion correction of the blood TAC without arterial spillover correction yielded in seven examinations solutions inconsistent with physiology. Correction of arterial spillover increased the fitting accuracy and yielded consistent results in all patients. Generation of an AIF from PET image data was investigated as an alternative to arterial blood sampling and was shown to have an intrinsic potential to determine the AIF noninvasively and reproducibly. The AIF extracted from a VOI in a dynamic PET scan was similar in shape to the blood AIF but yielded significantly higher tissue perfusion values (mean of 104.0 ± 52.0%) and lower partition coefficients (-31.6 ± 24.2%). The perfusion values and partition coefficients determined with the VOI technique have to be corrected in order to compare the results with those of studies using a blood AIF.

  3. Postoperative treatment of glioblastoma multiforme with radiation therapy plus concomitant and adjuvant temozolomide : A mono-institutional experience of 215 patients

    Directory of Open Access Journals (Sweden)

    Pramod Kumar Julka

    2013-01-01

    Full Text Available Objective: To study the clinical results and prognostic factors of patients with glioblastoma multiforme (GBM treated by postoperative radiation therapy (PORT and concomitant temozolomide followed by adjuvant temozolomide. Methods: From 2005 to 2008, 215 patients (median age 48 years with GBM were treated with PORT plus temozolomide chemotherapy. Radiation therapy (RT was employed with a dose of 60 Gy in 30 fractions over 6 weeks by conventional fractionation with concomitant temozolomide (75 mg/m 2 /day. Adjuvant therapy consisted of 6 cycles of temozolomide (150 mg/m 2 for 5 days, 28 days cycle. The primary end point of the study was overall survival (OS, and the secondary end points were progression free survival (PFS and toxicity. OS was determined with respect to different variables to study the prognostic significance. Results: Median follow up was 11 months (range 2-50 months. Median OS and PFS were 13 months and 11 months respectively. The 1-year and 2-year OS was 44% and 18% respectively. There was no statistical significant impact of age, sex, KP score, anatomical location and extent of surgery. Presentation without seizures (on univariate analysis and 6 cycles of adjuvant temozolomide therapy (on univariate as well as multivariate analysis were found significant prognostic factors. Sixteen patients developed grade III-IV neutropenia/thrombocytopenia during the course of RT. Conclusion: Our results authenticate the role of concomitant and adjuvant temozolomide chemotherapy in combination with PORT for the management of GBM patients. We strongly recommend complete 6 cycle of adjuvant temozolomide since it significantly improved the survival in our study.

  4. No association between exposure to perfluorinated compounds and congenital cryptorchidism: a nested case-control study among 215 boys from Denmark and Finland.

    Science.gov (United States)

    Vesterholm Jensen, Dorte; Christensen, Jeppe; Virtanen, Helena E; Skakkebæk, Niels E; Main, Katharina M; Toppari, Jorma; Veje, Christine W; Andersson, Anna-Maria; Nielsen, Flemming; Grandjean, Philippe; Jensen, Tina Kold

    2014-01-01

    Geographical differences in the occurrence of diseases in male reproductive organs, including malformation in reproductive tract, between Denmark and Finland have been reported. The reason for these differences is unknown, but differences in exposure to chemicals with endocrine-disrupting abilities have been suggested. Among these chemicals are perfluoro-alkylated substances (PFASs), a group of water- and grease-repellent chemicals used in outdoor clothes, cookware, food packaging, and textiles. In this study, we, therefore, investigated differences in PFAS exposure levels between Denmark and Finland and the association between cord blood PFAS levels and congenital cryptorchidism. Boys from a joint ongoing prospective birth cohort study were included. We analyzed PFAS levels in cord blood serum samples collected from 29 Danish boys with congenital cryptorchidism, 30 healthy Danish matched controls recruited from 1997 to 2001, 30 Finnish cases, and 78 Finnish healthy matched controls recruited from 1997 to 1999. Additionally, 48 Finnish cases recruited from 2000 to 2002 were included. Perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) were detected in all the 215 Danish and Finnish cord blood samples with significantly higher levels being observed in the Danish samples (medians: PFOA, 2.6 ng/ml and PFOS, 9.1 ng/ml) than in the Finnish samples (medians: PFOA, 2.1 ng/ml and PFOS, 5.2 ng/ml). We found no associations between cord blood PFOA and PFOS levels and congenital cryptorchidism after adjustment for confounders. Our data indicate that women in Denmark and Finland are generally exposed to PFOA and PFOS but there are differences in exposure levels between countries. We found no statistically significant association between cord blood PFOA and PFOS levels and congenital cryptorchidism; however, our study was small and larger studies are warranted. PMID:24218628

  5. NuSTAR study of hard X-ray morphology and spectroscopy of PWN G21.5–0.9

    Energy Technology Data Exchange (ETDEWEB)

    Nynka, Melania; Hailey, Charles J.; Gotthelf, Eric V.; Mori, Kaya; Perez, Kerstin [Columbia Astrophysics Laboratory, Columbia University, New York, NY 10027 (United States); Reynolds, Stephen P. [Physics Department, NC State University, Raleigh, NC 27695 (United States); An, Hongjun [Department of Physics, McGill University, Rutherford Physics Building, 3600 University Street, Montreal, Quebec H3A 2T8 (Canada); Baganoff, Frederick K. [Center for Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139-4307 (United States); Boggs, Steven E.; Krivonos, Roman; Zoglauer, Andreas [Space Sciences Laboratory, University of California, Berkeley, CA 94720 (United States); Christensen, Finn E. [DTU Space, National Space Institute, Technical University of Denmark, Elektrovej 327, DK-2800 Lyngby (Denmark); Craig, William W. [Lawrence Livermore National Laboratory, Livermore, CA 94550 (United States); Grefenstette, Brian W.; Harrison, Fiona A.; Madsen, Kristin K. [Cahill Center for Astronomy and Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Stern, Daniel [Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 (United States); Wik, Daniel R.; Zhang, William W. [NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States)

    2014-07-01

    We present NuSTAR high-energy X-ray observations of the pulsar wind nebula (PWN)/supernova remnant G21.5–0.9. We detect integrated emission from the nebula up to ∼40 keV, and resolve individual spatial features over a broad X-ray band for the first time. The morphology seen by NuSTAR agrees well with that seen by XMM-Newton and Chandra below 10 keV. At high energies, NuSTAR clearly detects non-thermal emission up to ∼20 keV that extends along the eastern and northern rim of the supernova shell. The broadband images clearly demonstrate that X-ray emission from the North Spur and Eastern Limb results predominantly from non-thermal processes. We detect a break in the spatially integrated X-ray spectrum at ∼9 keV that cannot be reproduced by current spectral energy distribution models, implying either a more complex electron injection spectrum or an additional process such as diffusion compared to what has been considered in previous work. We use spatially resolved maps to derive an energy-dependent cooling length scale, L(E)∝E{sup m} with m = –0.21 ± 0.01. We find this to be inconsistent with the model for the morphological evolution with energy described by Kennel and Coroniti. This value, along with the observed steepening in power-law index between radio and X-ray, can be quantitatively explained as an energy-loss spectral break in the simple scaling model of Reynolds, assuming particle advection dominates over diffusion. This interpretation requires a substantial departure from spherical magnetohydrodynamic, magnetic-flux-conserving outflow, most plausibly in the form of turbulent magnetic-field amplification.

  6. Angular dependence of the disorder crossover in the vortex lattice of Bi{sub 2.15}Sr{sub 1.85}CaCu{sub 2}O{sub 8+{delta}} by muon spin rotation and torque magnetometry

    Energy Technology Data Exchange (ETDEWEB)

    Aegerter, C.M.; Hofer, J.; Savic, I.M.; Keller, H. [Physik-Institut der Universitaet Zuerich, CH-8057 Zuerich (Switzerland); Lee, S.L.; Ager, C. [School of Physics and Astronomy, University of St. Andrews, St. Andrews, Fife KY169SS (United Kingdom); Lloyd, S.H.; Forgan, E.M. [School of Physics and Space Research, University of Birmingham, Birmingham B15 2TT (United Kingdom)

    1998-01-01

    Using the techniques of muon spin rotation and torque magnetometry, we investigate the crossover field B{sub cr} in Bi{sub 2.15}Sr{sub 1.85}Ca{sub 1}Cu{sub 2}O{sub 8+{delta}} at which the vortex lattice becomes disordered along the field direction. It is found that B{sub cr} scales as the projection of the applied field along the perpendicular to the superconducting planes. This has the implication that a field large enough to give a disordered lattice when applied perpendicular to the planes, can give a well-ordered vortex-line lattice for angles of the field to the c axis greater than a critical value. {copyright} {ital 1998} {ital The American Physical Society}

  7. Oceanographic profile data collected from sound velocimeter - moving vessel profiler casts aboard BLAKE as part of project OPR-J377-KR2-15 in the Gulf of Mexico from 2015-06-03 to 2015-10-30 (NCEI Accession 0139358)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0139358 includes physical and profile data collected aboard the BLAKE during project OPR-J377-KR2-15 in the Gulf of Mexico from 2015-06-03 to...

  8. Purification of selenium from thorium, uranium, radium, actinium and potassium impurities for low background measurements

    International Nuclear Information System (INIS)

    A technique of selenium purification from 232Th, 238U, 226,228Ra, 227Ac and 40K was developed. This technique is simple to perform and employs a minimum number of highly pure reagents (bidistilled water, nitric acid). Operations carried out during purification (elution, evaporation) practically exclude losses of the target product (chemical yields of Se > 99%). A test purification of 100 g of selenium was carried out using this technique. The efficiency of this technique was confirmed by low background gamma spectrometry of the purified selenium sample. Distribution coefficients of Th, U, Ra and Ac on DOWEX 50W- x 8 cation-exchange resin at different concentrations of selenium and nitric acid were experimentally determinated. Instrumental neutron activation analysis of bidistilled water, deionized water and nitric acid was performed. (orig.)

  9. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228

    Energy Technology Data Exchange (ETDEWEB)

    Aleissa, Khalid A.; Almasoud, Fahad I.; Islam, Mohammed S. [Atomic Energy Research Institute, King Abdul Aziz City for Science and Technology, P.O. Box 6086, Riyadh 11442 (Saudi Arabia); L' Annunziata, Michael F. [IAEA Expert, Montague Group, P.O. Box 5033, Oceanside, CA 92052-5033 (United States)], E-mail: mlannunziata@cox.net

    2008-12-15

    The activities of {sup 228}Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide {sup 228}Ac. The radium was pre-concentrated on MnO{sub 2} and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter {sup 228}Ra({sup 228}Ac), the daughter nuclide {sup 228}Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by {sup 228}Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9{+-}0.1% was measured for {sup 228}Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317{+-}0.013 cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1 g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of {sup 228}Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for {sup 228}Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure {sup 228}Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  10. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228.

    Science.gov (United States)

    Aleissa, Khalid A; Almasoud, Fahad I; Islam, Mohammed S; L'Annunziata, Michael F

    2008-12-01

    The activities of (228)Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide (228)Ac. The radium was pre-concentrated on MnO(2) and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter (228)Ra((228)Ac), the daughter nuclide (228)Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by (228)Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9+/-0.1% was measured for (228)Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317+/-0.013cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of (228)Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for (228)Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure (228)Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  11. Purification of selenium from thorium, uranium, radium, actinium and potassium impurities for low background measurements

    Energy Technology Data Exchange (ETDEWEB)

    Rakhimov, A.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz); Warot, G. [CEA-CNRS, Modane (France). Laboratoire Souterrain de Modane (LSM); Karaivanov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Institute for Nuclear Research and Nuclear Energy (INRNE), Sofia (Bulgaria); Kochetov, O.I.; Lebedev, N.A.; Filosofov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Mukhamedshina, N.M.; Sadikov, I.I. [Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz)

    2013-07-01

    A technique of selenium purification from {sup 232}Th, {sup 238}U, {sup 226,228}Ra, {sup 227}Ac and {sup 40}K was developed. This technique is simple to perform and employs a minimum number of highly pure reagents (bidistilled water, nitric acid). Operations carried out during purification (elution, evaporation) practically exclude losses of the target product (chemical yields of Se > 99%). A test purification of 100 g of selenium was carried out using this technique. The efficiency of this technique was confirmed by low background gamma spectrometry of the purified selenium sample. Distribution coefficients of Th, U, Ra and Ac on DOWEX 50W- x 8 cation-exchange resin at different concentrations of selenium and nitric acid were experimentally determinated. Instrumental neutron activation analysis of bidistilled water, deionized water and nitric acid was performed. (orig.)

  12. Linear free energy relationship applied to trivalent cations with lanthanum and actinium oxide and hydroxide structure

    International Nuclear Information System (INIS)

    Linear free energy relationships for trivalent cations with crystalline M2O3 and, M(OH)3 phases of lanthanides and actinides were developed from known thermodynamic properties of the aqueous trivalent cations, modifying the Sverjensky and Molling equation. The linear free energy relationship for trivalent cations is as ΔGf,MvX0=aMvXΔGn,M3+0+bMvX+βMvXrM3+, where the coefficients aMvX, bMvX, and βMvX characterize a particular structural family of MvX, rM3+ is the ionic radius of M3+ cation, ΔGf,MvX0 is the standard Gibbs free energy of formation of MvX and ΔGn,M3+0 is the standard non-solvation free energy of the cation. The coefficients for the oxide family are: aMvX=0.2705, bMvX=-1984.75 (kJ/mol), and βMvX=197.24 (kJ/molnm). The coefficients for the hydroxide family are: aMvX=0.1587, bMvX=-1474.09 (kJ/mol), and βMvX=791.70 (kJ/molnm).

  13. Actinium: A RESTful Runtime Container for Scriptable Internet of Things Applications

    OpenAIRE

    Kovatsch, Matthias; Lanter, Martin; Duquennoy, Simon

    2012-01-01

    Programming Internet of Things (IoT) applications is challenging because developers have to be knowledgeable in various technical domains, from low-power networking, over embedded operating systems, to distributed algorithms. Hence, it will be challenging to find enough experts to provide software for the vast number of expected devices, which must also be scalable and particularly safe due to the connection to the physical world. To remedy this situation, we propose an architecture that pr...

  14. Reflection asymmetry in odd-A and odd-odd actinium nuclei

    International Nuclear Information System (INIS)

    Theoretical calculations and measurements indicate that octupole correlations are at a maximum in the ground states of the odd-proton nuclei Ac and Pa. It has been expected that odd-odd nuclei should have even larger amount of octupole-octupole correlations. We have recently made measurements on the structure of 224Ac. Although spin and parity assignments could not be made, two bands starting at 354.1 and 360.0 keV have properties characteristic of reflection asymmetric shape. These two bands have very similar rotational constants and also similar alpha decay rates, which suggest similarity between the wavefunctions of these bands. These signatures provide evidence for octupole correlations in these nuclides

  15. Bestimmung der Ionisationsenergie von Actinium und Ultraspurenanalyse von Plutonium mit resonanter Ionisationsmassenspektrometrie (RIMS)

    OpenAIRE

    Waldek, Achim Marcus

    2001-01-01

    ZusammenfassungDie Resonanzionisationsmassenspektrometrie (RIMS) verbindet hohe Elementselektivität mit guter Nachweiseffizienz. Aufgrund dieser Eigenschaften ist die Methode für Ultraspurenanalyse und Untersuchungen an seltenen oder schwer handhabbaren Elementen gut geeignet. Für RIMS werden neutrale Atome mit monochromatischem Laserlicht ein- oder mehrfach resonant auf energetisch hoch liegende Niveaus angeregt und anschließend durch einen weiteren Laserstrahl oder durch ein elektrisches Fe...

  16. New method for large scale production of medically applicable Actinium-225 and Radium-223

    International Nuclear Information System (INIS)

    Alpha-emitters (211At, 212Bi, 213Bi, 223Ra, 225Ac) are promising for targeted radiotherapy of cancer. Only two alpha decays near a cell membrane result in 50% death of cancer cell and only a single decay inside the cell is required for this. 225Ac may be used either directly or as a mother radionuclide in 213Bi isotope generator. Production of 225Ac is provided by three main suppliers - Institute for Transuranium Elements in Germany, Oak Ridge National Laboratory in USA and Institute of Physics and Power Engineering in Obninsk, Russia. The current worldwide production of 225Ac is approximately 1.7 Ci per year that corresponds to only 100-200 patients that could be treated annually. The common approach for 225Ac production is separation from mother 229Th or irradiation of 226Ra with protons in a cyclotron. Both the methods have some practical limitations to be applied routinely. 225Ac can be also produced by irradiation of natural thorium with medium energy protons . Cumulative cross sections of 225Ac, 227Ac, 227Th, 228Th formations have been obtained recently. Thorium targets (1-9 g) were irradiated by 114-91 MeV proton beam (1-50 μA) at INR linear accelerator. After dissolution in 8 M HNO3 + 0.004 M HF thorium was removed by double LLX by HDEHP in toluene (1:1). Ac and REE were pre-concentrated and separated from Ra and most fission products by DGA-Resin (Triskem). After washing out by 0.01 M HNO3 Ac was separated from REE by TRU Resin (Triskem) in 3 M HNO3 media. About 6 mCi 225Ac were separated in hot cell with chemical yield 85%. The method may be upscaled for production of Ci amounts of the radionuclide. The main impurity is 227Ac (0.1% at the EOB) but it does not hinder 225Ac from being used for medical 225Ac/213Bi generators. (author)

  17. Best Practices, Creative Ideas, Linking all data, Exploring the Future:The Summary of the 12th Advanced Digital Library Seminar & Lib 2.15 Conference%知行合一 创新创意 关联无限 开拓未来——第12届数字图书馆前沿问题高级研讨班暨Lib2.15会议综述

    Institute of Scientific and Technical Information of China (English)

    杨薇; 陈晓亮; 黄国凡

    2015-01-01

    2015年7月6日至8日,第12届数字图书馆前沿问题高级研讨班暨Lib2.15会议在厦门大学图书馆举行.三天的议程涵盖数据服务、关联数据、数字人文、图书馆合作及行业未来等五项分主题内容.通过对会议报告和分享案例的梳理,提出图书馆的未来发展取决于树立终身学习的思想,寻求更大范围的合作,项目实施体现人的价值以及无边界创新创意.

  18. IL-32对 HepG22.15细胞凋亡的影响∗%Effect of Interleukin-32 on the Apoptosis of HepG2 2.15 Cells

    Institute of Scientific and Technical Information of China (English)

    潘兴飞; 孙媛丽; 贺琪; 尹旺春; 符明鹏; 沈关心; 朱慧芬

    2016-01-01

    Objective To investigate the effect of interleukin-32(IL-32)on the apoptosis of HepG2 2.15 cells in which the whole genome of HBV was inserted and persistently expressed.Methods HepG2 cells or HepG2 2.15 cells were treated with different concentrations of IL-32(0-0.4μg/mL).Forty-eight hours later,cell proliferation was assayed by MTT method.Cell apoptosis was assayed by flow cytometry(FCM)after treatment of both cells with IL-32 for 48 h,and then with annexin Ⅴ and PI at room temperature in the dark for 15 min.Subsequently,HepG2 cells or HepG2 2.15 cells were co-cultured with IL-32 at 0.2μg/mL.They were collected at 0,12,24 h,respectively.Activated caspase-3 expression levels were assayed by using Human Active Caspase-3 Immunoassay Kit.Caspase-3 enzyme activities were detected by Caspase-3 Colorimetric Assay Kit.Results IL-32 could inhibit the proliferation of HepG2 cells and HepG2 2.15 cells,and induce their apoptosis in a dose-dependent man-ner.Activated caspase-3 expression levels and caspase-3 enzyme activities were increased in both HepG2 and HepG2 2.15 cells co-cultured with IL-32.Conclusion IL-32 play an anti-viral role by activating the caspase-3 pathway and inducing the apoptosis of HBV-infected hepatocytes,and it is involved in the liver damage of chronic hepatitis B at the same time.%目的:研究白细胞介素-32(IL-32)对乙型肝炎病毒(HBV)感染的肝细胞(HepG22.15细胞,插入 HBV 全基因组,持续表达)凋亡的影响。方法将不同浓度的 IL-32(0~0.4μg/mL)蛋白质与 HepG2细胞、HepG22.15细胞共培养,48 h后用 MTT检测细胞增殖。收集与 IL-32共培养48 h的 HepG2细胞、HepG22.15细胞,加入 Annexin Ⅴ和PI,室温避光孵育15 min,流式细胞仪检测细胞凋亡。将 IL-32(0.2μg/mL)与 HepG2细胞、HepG22.15细胞共培养,分别于0、12、24 h 后收集细胞,Human Active Caspase-3 Immunoassay 检测试剂盒检测活化的 Caspase-3表达水平, Caspase-3 Colorimetric检测试剂盒检测 Caspase-3

  19. Molecular dynamics simulation and free energy landscape methods in probing L215H, L217R and L225M βI-tubulin mutations causing paclitaxel resistance in cancer cells.

    Science.gov (United States)

    Tripathi, Shubhandra; Srivastava, Gaurava; Sharma, Ashok

    2016-08-01

    Drug resistance poses a threatening challenge for mankind, as the development of resistance to already well-established drugs causes serious therapeutic problems. Resistance to paclitaxel (Ptxl), a complex diterpenoid working as microtubule stabilizer, is one such issue in cancer treatment. Microtubule stabilizer drugs, stabilises microtubules upon binding to β-tubulin subunit of tubulin heterodimer thus causing mitotic arrest leading to death of cancer cell. Leucine point mutations viz. L215H, L217R, and L225M were reported for Ptxl resistance in various cancers. In the current study, molecular mechanism of these resistance causing mutations was explored using molecular docking, molecular dynamics (MD) simulation, binding energy estimation (MMPBSA), free energy decomposition, principle component analysis (PCA) and free energy landscape (FEL) methods. A total of five systems including unbound βI-tubulin (Apo), docked wild+Ptxl, L215H+Ptxl, L217R+Ptxl and L225M+Ptxl were prepared, and 50 ns MD simulation was performed for each system. Binding energy estimation indicated that leucine mutation reduces the binding affinity of Ptxl in mutant types (MTs) as compared to wild type (WT). Further, in contrast to WT Ptxl interactions with the M-loop (PHE270-VAL286), S6-S7 loop and H9-H10 were significantly altered in MTs. Results showed that in MTs, Ptxl had weak interaction with M-loop residues, while having strong affinity with S6-S7 loop and H6-H7 loop. Moreover, PCA and FEL analysis revealed that M-loop flexible region (THR274-LEU284) was strongly bound with Ptxl in WT preventing its flexible movement and the causing factor for microtubule stabilization. In MTs due to poor interaction with Ptxl, M-loop flexible region retains its flexibility, therefore unable to stabilize microtubule. This study will give an insight into the importance of M-loop flexible region interaction with Ptxl for microtubule stabilization. In addition, it clearly provides the molecular basis of

  20. Paleomagnetism of Basaltic Lava Flows in Coreholes ICPP 213, ICPP-214, ICPP-215, and USGS 128 Near the Vadose Zone Research Park, Idaho Nuclear Technology and Engineering Center, Idaho National Engineering and Environmental Laboratory, Idaho

    Science.gov (United States)

    Champion, Duane E.; Herman, Theodore C.

    2003-01-01

    A paleomagnetic study was conducted on basalt from 41 lava flows represented in about 2,300 ft of core from coreholes ICPP-213, ICPP-214, ICPP-215, and USGS 128. These wells are in the area of the Idaho Nuclear Technology and Engineering Center (INTEC) Vadose Zone Research Park within the Idaho National Engineering and Environmental Laboratory (INEEL). Paleomagnetic measurements were made on 508 samples from the four coreholes, which are compared to each other, and to surface outcrop paleomagnetic data. In general, subhorizontal lines of correlation exist between sediment layers and between basalt layers in the area of the new percolation ponds. Some of the basalt flows and flow sequences are strongly correlative at different depth intervals and represent important stratigraphic unifying elements. Some units pinch out, or thicken or thin even over short separation distances of about 1,500 ft. A more distant correlation of more than 1 mile to corehole USGS 128 is possible for several of the basalt flows, but at greater depth. This is probably due to the broad subsidence of the eastern Snake River Plain centered along its topographic axis located to the south of INEEL. This study shows this most clearly in the oldest portions of the cored sections that have differentially subsided the greatest amount.

  1. Diffuse phase transition and impedance spectroscopy analysis of Ba2.15- x Na0.7+ x Nb5- x W x O15 ( x = 0.25) ferroelectric ceramic

    Science.gov (United States)

    Neqali, A.; Belboukhari, A.; Bensaid, H.; El Bouari, A.; Bih, L.; Alimoussa, A.; Habouti, S.; Mezzane, D.

    2016-06-01

    A new rare earth ferroelectric tetragonal tungsten bronze compound with general formula Ba2.15- xNa0.7+ xNb5- xWxO15 ( x = 0.25) was elaborated as ceramic and investigated using X-ray diffraction and dielectric measurements. The results show a tetragonal phase with the space group P4bm. Special emphasis was done to characterize diffuse phase transition (DPT) that occurs close to 472 °C. Using dielectric measurements in a frequency range of (10 Hz-1 MHz) and in a temperature range (25-550 °C), we have shown that the real part of the permittivity close to DPT is well described by Santos-Eiras phenomenological model. Space charge polarization, relaxation phenomena and free charges conductivity have been analyzed using dielectric, impedance spectroscopy and Nyquist plots showed non-Debye (polydispersive) type relaxation. In paraelectric phase, the Arrhenius activation energies were determined. Frequency dependence of ac conductivity at different temperatures follows the Jonscher's universal law (Jonscher et al. in J Mater Sci 20:4431, 1985).

  2. Effect of Sol-Gel Ageing Time on Three Dimensionally Ordered Macroporous Structure of 80SiO2-15CaO-5P2O5 Bioactive Glasses

    Directory of Open Access Journals (Sweden)

    Thanida CHAROENSUK

    2014-04-01

    Full Text Available Three dimensionally ordered macroporous bioactive glasses (3DOM-BGs, namely 80SiO2-15CaO-5P2O5, were synthesized by sol-gel method. PMMA colloidal crystals and non-ionic block copolymers P123 were used as cotemplates. The amorphous 3DOM-BGs had skeletal walls enclosing macropores. Such structure resulted from octahedral and tetrahedral holes of the face-centered cubic (fcc closest packed PMMA templates and windows interconnecting through macropores network. The thicknesses of the walls were around 50 nm – 80 nm and the windows were 90 nm – 110 nm in diameter. These wall thickness is increased by with an increase in ageing time up to 24 h and  then gradually reduced with further increase in aging time. Vibration bands of Si–O–Si and P–O were evident in infrared spectra which are in agreement with EDS spectra indicating Si, P and Ca compositions. After in vitro bioactivity testing by soaking 3DOM-BGs in simulated body fluid at 37 °C, the crystallization of amorphous calcium phosphate layers compatible to the bone component of hydroxyl carbonate apatite were rapidly formed within 3 h. These results indicated that these 3DOM-BGs resembled ideal bone implant materials.DOI: http://dx.doi.org/10.5755/j01.ms.20.1.4755

  3. Radioimmunotherapy for first-line and relapse treatment of aggressive B-cell non-Hodgkin lymphoma: an analysis of 215 patients registered in the international RIT-Network

    International Nuclear Information System (INIS)

    Very few reliable clinical data about the use of radioimmunotherapy in aggressive B-cell lymphoma exist. Patients with aggressive B-cell lymphoma registered in the international RIT-Network were analysed with regard to prior treatment, response and side effects. The RIT-Network is a web-based registry that collects observational data from radioimmunotherapy-treated patients with malignant lymphoma across 13 countries. This analysis included 215 with aggressive B-cell lymphoma out of 232 patients registered in the RIT-Network. Histological subtypes were as follows: 190 diffuse large B-cell, 15 primary mediastinal, 9 anaplastic large cell, and 1 intravascular lymphoma. The median age of the patients was 62 years (range 17 - 88), with 27 % above the age of 70 years. Radioimmunotherapy was mainly used as consolidation after first-line or second-line chemotherapy (56.1 %), as part of third-line to eighth-line therapy for relapse (16.4 %), and in refractory disease (12.2 %). Grade IV neutropenia and thrombopenia and grade III anaemia were observed. The median time to recovery of blood count was 81 days (range 0 - 600 days). The overall response rate was 63.3 %. The complete response rate was 76.4 % in patients treated as part of first-line therapy, and 44.3 % in patients with relapse. Mean overall survival in first-line therapy patients was 32.7 months and 14.0 months in patients with relapse or refractory disease, respectively. Most patients with aggressive B-cell lymphoma in the RIT-Network received radioimmunotherapy as consolidation after first-line therapy with excellent complete remission and overall survival rates compared to published data. In relapsed aggressive B-cell lymphoma, radioimmunotherapy is a safe and feasible treatment leading to satisfactory response rates with acceptable toxicity. (orig.)

  4. Radioimmunotherapy for first-line and relapse treatment of aggressive B-cell non-Hodgkin lymphoma: an analysis of 215 patients registered in the international RIT-Network

    Energy Technology Data Exchange (ETDEWEB)

    Hohloch, Karin; Lankeit, H.K.; Truemper, L. [Georg August University, Hematology and Oncology, Goettingen (Germany); Zinzani, P.L. [University of Bologna, Institute of Hematology and Medical Oncology ' ' L. e A. Seragnoli' ' , Bologna (Italy); Scholz, C.W. [Charite, University Berlin, Hematology, Oncology and Tumor Immunology, Berlin (Germany); Lorsbach, M.; Windemuth-Kieselbach, C. [Alcedis GmbH, Giessen (Germany)

    2014-08-15

    Very few reliable clinical data about the use of radioimmunotherapy in aggressive B-cell lymphoma exist. Patients with aggressive B-cell lymphoma registered in the international RIT-Network were analysed with regard to prior treatment, response and side effects. The RIT-Network is a web-based registry that collects observational data from radioimmunotherapy-treated patients with malignant lymphoma across 13 countries. This analysis included 215 with aggressive B-cell lymphoma out of 232 patients registered in the RIT-Network. Histological subtypes were as follows: 190 diffuse large B-cell, 15 primary mediastinal, 9 anaplastic large cell, and 1 intravascular lymphoma. The median age of the patients was 62 years (range 17 - 88), with 27 % above the age of 70 years. Radioimmunotherapy was mainly used as consolidation after first-line or second-line chemotherapy (56.1 %), as part of third-line to eighth-line therapy for relapse (16.4 %), and in refractory disease (12.2 %). Grade IV neutropenia and thrombopenia and grade III anaemia were observed. The median time to recovery of blood count was 81 days (range 0 - 600 days). The overall response rate was 63.3 %. The complete response rate was 76.4 % in patients treated as part of first-line therapy, and 44.3 % in patients with relapse. Mean overall survival in first-line therapy patients was 32.7 months and 14.0 months in patients with relapse or refractory disease, respectively. Most patients with aggressive B-cell lymphoma in the RIT-Network received radioimmunotherapy as consolidation after first-line therapy with excellent complete remission and overall survival rates compared to published data. In relapsed aggressive B-cell lymphoma, radioimmunotherapy is a safe and feasible treatment leading to satisfactory response rates with acceptable toxicity. (orig.)

  5. 29 CFR 825.215 - Equivalent position.

    Science.gov (United States)

    2010-07-01

    ... schedule. (3) The employee must have the same or an equivalent opportunity for bonuses, profit-sharing, and... equivalent job with the same or equivalent pay, benefits, and terms and conditions of employment does not extend to de minimis, intangible, or unmeasurable aspects of the job....

  6. 7 CFR 215.14 - Nondiscrimination.

    Science.gov (United States)

    2010-01-01

    .... When different types of milk are served to children, (a) a uniform price for each type of milk served... Department's agreements with State agencies, the State agencies' agreements with School Food Authorities and child-care institutions and the FNSRO agreements with School Food Authorities administering...

  7. 40 CFR 46.215 - Enforcement.

    Science.gov (United States)

    2010-07-01

    ... the cost of the activity or action not in compliance; (3) Wholly or partly terminate the fellowship... will provide you an opportunity for hearing, appeal, or other administrative proceeding to which you... implementing regulations (2 CFR part 1532)....

  8. 12 CFR 215.2 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... any corporation, partnership, trust (business or otherwise), association, joint venture, pool syndicate, sole proprietorship, unincorporated organization, or any other form of business entity not... that person or the funds or services of which will benefit that person. (o) Subsidiary has the...

  9. 12 CFR 215.4 - General prohibitions.

    Science.gov (United States)

    2010-01-01

    ... corporation wholly owned directly or indirectly by the United States; (C) Extensions of credit secured by a... bank or insider of its affiliates unless the extension of credit: (i) Is made on substantially the same... the member bank and, in the case of extensions of credit to an insider of its affiliates, is...

  10. 2 CFR 215.52 - Financial reporting.

    Science.gov (United States)

    2010-01-01

    ... whether the report shall be on a cash or accrual basis. If the Federal awarding agency requires accrual information and the recipient's accounting records are not normally kept on the accrual basis, the recipient shall not be required to convert its accounting system, but shall develop such accrual...

  11. 2 CFR 215.27 - Allowable costs.

    Science.gov (United States)

    2010-01-01

    ... CFR part 230, “Cost Principles for Non-Profit Organizations (OMB Circular A-122).” The allowability of... CFR part 220, “Cost Principles for Educational Institutions (OMB Circular A-21).” The allowability of costs incurred by hospitals is determined in accordance with the provisions of appendix E of 45 CFR...

  12. 2 CFR 215.2 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... acquisition cost in accordance with the recipient's regular accounting practices. (d) Advance means a payment...'s or subrecipient's contract. (i) Cost sharing or matching means that portion of project or program... awarding agency determines to be unallowable, in accordance with the applicable Federal cost principles...

  13. 32 CFR 215.4 - Legal considerations.

    Science.gov (United States)

    2010-07-01

    ... national entity under the Federal Constitution—to insure the preservation of public order and the carrying.... (a) Under the Constitution and laws of the United States, the protection of life and property and the... necessary authority to enforce the laws. The Federal Government may assume this responsibility and...

  14. 2 CFR 215.44 - Procurement procedures.

    Science.gov (United States)

    2010-01-01

    ... metric system of measurement. (vi) Preference, to the extent practicable and economically feasible, for... performance, financial and technical resources or accessibility to other necessary resources. In...

  15. 2 CFR 215.22 - Payment.

    Science.gov (United States)

    2010-01-01

    ...) Payment methods shall minimize the time elapsing between the transfer of funds from the United States.... Payment methods of State agencies or instrumentalities shall be consistent with Treasury-State CMIA agreements or default procedures codified at 31 CFR part 205. (b) Recipients are to be paid in...

  16. 7 CFR 215.2 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... the Child Nutrition Act of 1966. Adults means those persons not included under the definition of... referenced in this definition, see 5 CFR 1310.3. Child and Adult Care Food Program means the program... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF...

  17. Purification of radium-226 for the manufacturing of actinium-225 in a cyclotron for alpha-immunotherapy; Radium-Aufreinigung zur Herstellung von Actinium-225 am Zyklotron fuer die Alpha-Immuntherapie

    Energy Technology Data Exchange (ETDEWEB)

    Marx, Sebastian Markus

    2014-09-23

    The thesis describes the development of methods for the purification of Ra-226. The objective was to obtain the radionuclide in the quality that is needed to be used as starting material in the manufacturing process for Ac-225 via proton-irradiated Ra-226. The radionuclide has been gained efficiently out of huge excesses of impurities. The high purity of the obtained radium affords its use as staring material in a pharmaceutical manufacturing process.

  18. Renal uptake of bismuth-213 and its contribution to kidney radiation dose following administration of actinium-225-labeled antibody

    Energy Technology Data Exchange (ETDEWEB)

    Schwartz, J; O' Donoghue, J A; Humm, J L [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); Jaggi, J S [Bristol-Myers Squibb, Plainsboro, NJ (United States); Ruan, S; Larson, S M [Nuclear Medicine Service Department of Radiology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); McDevitt, M; Scheinberg, D A, E-mail: schwarj1@mskcc.org [Molecular Pharmacology and Chemistry, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10065 (United States)

    2011-02-07

    Clinical therapeutic studies using {sup 225}Ac-labeled antibodies have begun. Of major concern is renal toxicity that may result from the three alpha-emitting progeny generated following the decay of {sup 225}Ac. The purpose of this study was to determine the amount of {sup 225}Ac and non-equilibrium progeny in the mouse kidney after the injection of {sup 225}Ac-huM195 antibody and examine the dosimetric consequences. Groups of mice were sacrificed at 24, 96 and 144 h after injection with {sup 225}Ac-huM195 antibody and kidneys excised. One kidney was used for gamma ray spectroscopic measurements by a high-purity germanium (HPGe) detector. The second kidney was used to generate frozen tissue sections which were examined by digital autoradiography (DAR). Two measurements were performed on each kidney specimen: (1) immediately post-resection and (2) after sufficient time for any non-equilibrium excess {sup 213}Bi to decay completely. Comparison of these measurements enabled estimation of the amount of excess {sup 213}Bi reaching the kidney ({gamma}-ray spectroscopy) and its sub-regional distribution (DAR). The average absorbed dose to whole kidney, determined by spectroscopy, was 0.77 (SD 0.21) Gy kBq{sup -1}, of which 0.46 (SD 0.16) Gy kBq{sup -1} (i.e. 60%) was due to non-equilibrium excess {sup 213}Bi. The relative contributions to renal cortex and medulla were determined by DAR. The estimated dose to the cortex from non-equilibrium excess {sup 213}Bi (0.31 (SD 0.11) Gy kBq{sup -1}) represented {approx}46% of the total. For the medulla the dose contribution from excess {sup 213}Bi (0.81 (SD 0.28) Gy kBq{sup -1}) was {approx}80% of the total. Based on these estimates, for human patients we project a kidney-absorbed dose of 0.28 Gy MBq{sup -1} following administration of {sup 225}Ac-huM195 with non-equilibrium excess {sup 213}Bi responsible for approximately 60% of the total. Methods to reduce renal accumulation of radioactive progeny appear to be necessary for the success of {sup 225}Ac radioimmunotherapy.

  19. Production of actinium, thorium and radium isotopes from natural thorium irradiated with protons up to 141 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Ermolaev, S.V.; Zhuikov, B.L.; Kokhanyuk, V.M.; Matushko, V.L. [Russian Academy of Sciences, Moscow (Russian Federation). Inst. of Nuclear Research; Kalmykov, S.N. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Chemistry Dept.; Aliev, R.A. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Skobeltsyn Inst. of Nuclear Physics; Tananaev, I.G.; Myasoedov, B.F. [Russian Academy of Sciences, Moscow (Russian Federation). A.N. Frumkin Inst. of Physical Chemistry and Electrochemistry

    2012-07-01

    Cross sections of {sup 225}Ac, {sup 227}Ac, {sup 227}Th and {sup 228}Th in thorium-232 targets irradiated with protons in the energy range 21-141 MeV have been measured. Based on these data, production yields of {sup 225}Ac and {sup 223}Ra in thick thorium targets have been calculated. It is possible to produce in proton energy range 60-140 MeV about 96 GBq (2.6 Ci) {sup 225}Ac per 10-d irradiation with 100 {mu}A proton beam current and 10-d decay, and much higher amount of {sup 223}Ra. The impurities of {sup 227}Ac and {sup 224}Ra are important and need to be assessed for further medical applications. (orig.)

  20. Effect of LuF3 on Stability and Infrared Property of 20GaF3-15InF3-20CdF2-15ZnF2-20PbF2-10SnF2 Glass%Lu3+掺杂对20GaF3-15InF3-20CdF2-15ZnF2-20PbF2-10SnF2玻璃抗析晶能力和红外吸收边的影响

    Institute of Scientific and Technical Information of China (English)

    朱基千; 李志国; 贺芸芬; 唐高; 白朝颖

    2005-01-01

    以20GaF3-15InF3-20CdG2-15ZnF2-20PbF2-10SnF2氟化镓玻璃为基玻璃,用LuF3替代等摩尔含量的PbF2,以改善玻璃的抗析晶能力.用差示扫描量热法研究了LuF3含量对玻璃热性能和抗析晶能力的影响,用红外透过光谱研究了LuF3含量对玻璃透红外性能的影响.结果表明:在玻璃中掺入摩尔分数为2%的LuF3可以获得最佳的抗析晶能力;LuF3的掺入对玻璃的透红外性能稍有影响,红外截止波长的变化范围仅在10.1μm~10.9μm之间.

  1. 4 CFR 21.5 - Protest issues not for consideration.

    Science.gov (United States)

    2010-01-01

    ... time limits set forth in § 21.2. (f) Protests which lack a detailed statement of the legal and factual... business size standards and North American Industry Classification System (NAICS) standards. Challenges...

  2. Dicty_cDB: SSE215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available id sequence kylflivflffifffiylyldkeasymvfk*EKIKEKNKNKKKKKKKKKKEKKKK--- ---VALPILPXIXVITIWLFVGFPLTVVGGIAGRRLS...efiei*nltki Frame B: kylflivflffifffiylyldkeasymvfk*EKIKEKNKNKKKKKKKKKKEKKKK--- ---rritnptxd*xhyhlvicwfpfdrr

  3. 24 CFR 572.215 - Implementation grants-eligible activities.

    Science.gov (United States)

    2010-04-01

    ... related to implementing the affirmative fair housing marketing strategy required under § 572.110. (k...) Homebuyer outreach and selection. Reasonable and necessary costs of marketing the program to...

  4. Dicty_cDB: VSI215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Translated Amino Acid sequence ex*c*rtrrrrsrrrgrrttrrrrr*k**rin*rsrrrfprtpirn*shtkr*rfr*thf wllcftrr*lhclwf...GXTXNPXEIGGTSXEXQXKXXLVLQQNGNXERLSXFXPDXGPNKKN Frame B: ex*c*rtrrrrsrrrgrrttrrrrr*k**rin*rsrrrfprtpirn*shtkr*rfr*thf wllcftr

  5. Dicty_cDB: CFE215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available , *** SEQUENCING IN PROGRESS ***, 20 unordered pieces. 48 0.12 1 CF974266 |CF974266.1 PSU_nep1G03 Defense-related ESTs from Cacao Lea...ves Theobroma cacao cDNA, mRNA sequence. 44 1.9 1 AC1450

  6. 33 CFR 104.215 - Vessel Security Officer (VSO).

    Science.gov (United States)

    2010-07-01

    ... have general knowledge, through training or equivalent job experience, in the following: (1) Those... reporting of accidents; (C) Proper usage of protective equipment and general knowledge of procedures for... regulations specified in 46 CFR Subchapter B; (iv) Successfully complete a Coast Guard-accepted VSO course;...

  7. 48 CFR 1352.215-75 - Evaluation criteria.

    Science.gov (United States)

    2010-10-01

    ... criteria. As prescribed in 48 CFR 1315.204-570(b)(2) and (3), insert the following provision: Evaluation... customer satisfaction. The Government reserves the right to assess the past performance of...

  8. 48 CFR 215.404-71-2 - Performance risk.

    Science.gov (United States)

    2010-10-01

    ...., foreign currency exchange rates and inflation rates). (2) Above normal conditions. (i) The contracting... may assign a lower than normal value in those cases where the technical risk is low. Indicators are... requires an unusually low degree of management involvement....

  9. 14 CFR 61.215 - Ground instructor privileges.

    Science.gov (United States)

    2010-01-01

    ... pilot flight review; and (3) A recommendation for a knowledge test required for the issuance of a sport... (CONTINUED) AIRMEN CERTIFICATION: PILOTS, FLIGHT INSTRUCTORS, AND GROUND INSTRUCTORS Ground Instructors § 61... sport pilot certificate, recreational pilot certificate, private pilot certificate, or...

  10. Dicty_cDB: AFA215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available sion library, 48 hours NaCl treatment prescreened for removal of highly abundant transcripts Mesembryanthemu...ant Lambda Uni-Zap XR expression library, 48 hours NaCl treatment prescreened for removal

  11. 12 CFR 347.215 - Exemptions from deposit insurance requirement.

    Science.gov (United States)

    2010-01-01

    ..., branches, agencies or wholly owned subsidiaries of the bank to determine its average deposits; (D) The... other branches, agencies or wholly owned subsidiaries of the bank) for the purpose of this paragraph (a... (including any affiliate thereof) has extended credit or provided other nondeposit banking services...

  12. 2 CFR 215.23 - Cost sharing or matching.

    Science.gov (United States)

    2010-01-01

    ... value of donated property shall be determined in accordance with the usual accounting policies of the... property recorded in the recipient's accounting records at the time of donation. (2) The current fair... the use of equipment, buildings or land, normally only depreciation or use charges for equipment...

  13. 48 CFR 215.304 - Evaluation factors and significant subfactors.

    Science.gov (United States)

    2010-10-01

    ... require use of the clause at FAR 52.219-9, Small Business Subcontracting Plan, other than those based on... extent to which offerors identify and commit to small business and historically black college or... addressing the extent of small business and historically black college or university and minority...

  14. 48 CFR 52.215-14 - Integrity of Unit Prices.

    Science.gov (United States)

    2010-10-01

    ...: acquisitions at or below the simplified acquisition threshold in FAR Part 2; construction or architect-engineer...; commercial items; and petroleum products. (End of clause) Alternate I (OCT 1997). As prescribed in...

  15. Dicty_cDB: SSB215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available mRNA sequence. 48 1e-06 2 BQ476340 |BQ476340.1 curculio3e06.b Curculio glandium ...cDNA Curculio glandium cDNA clone curculio3e06 5', mRNA sequence. 46 0.38 1 BQ476622 |BQ476622.1 curculio3e0...6.g Curculio glandium cDNA Curculio glandium cDNA clone curculio3e06 3', mRNA sequence. 46 0.38 1 AC132016 |

  16. 48 CFR 215.404-71-4 - Facilities capital employed.

    Science.gov (United States)

    2010-10-01

    ... Forms CASB-CMF and cost of money factors (48 CFR 9904.414 and FAR 31.205-10); and (2) DD Form 1861... REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION...D. Indicators are— (A) Allocations of capital apply predominantly to commercial item lines;...

  17. Dicty_cDB: CFJ215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available xpressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera ...cDNA clone VVB066D06 5, mRNA sequence. 64 4e-07 2 CD719214 |CD719214.1 VVB158G03_410335 An expressed sequence tag database for abioti....1 VVD157G07_379711 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. C...quence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone ...decarboxylase isozyme 3 mRNA, complete cds. 58 2e-07 2 CD008053 |CD008053.1 VVB082D05_335234 An expressed se

  18. 12 CFR 215.1 - Authority, purpose, and scope.

    Science.gov (United States)

    2010-01-01

    ... executive officer, director, or principal shareholder of the member bank, of any company of which the member... of credit made to an executive officer, director, or principal shareholder of a bank (or to a related interest of such person) by a correspondent bank also are subject to restrictions set forth in 12...

  19. Dicty_cDB: VSD215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available msktavcvikgekvngvvkftqenkdspvtvnyditglekgehgfh vhafgdttngcvsagphfnpfgknhgapsdedrhvgdlgnivadgesntkgtisdkiisl fgeht...itglekgehgfh vhafgdttngcvsagphfnpfgknhgapsdedrhvgdlgnivadgesntkgtisdkiisl fgehtivgrtmvvhadqddlgkggkpdslttgaa

  20. 46 CFR 10.215 - Medical and physical requirements.

    Science.gov (United States)

    2010-10-01

    ... infarctions, psychiatric disorders, and convulsive disorders. (2) Food handlers are not required to submit to... complete annual medical exams and, unless exempt per 46 CFR 16.220, pass annual chemical tests for... conducted by a licensed medical doctor in accordance with the physical exam requirements in 46 CFR...

  1. AB215. Penis transplant: a long way to success

    OpenAIRE

    Li, Jinhong; Yuan, Jiuhong

    2016-01-01

    Objective Transplant seems to be an ideal and mature treatment for some late-stage diseases. The first penis transplant operation was performed in China about 9 years ago and recently, a piece of news reporting another successful penis transplant in South Africa had spread throughout the world. Overall, penis transplant was seldom reported. Methods PubMed, ISI Web of Science and Google Scholar databases (until January 2016) were searched for relevant publications on penis transplant. Results ...

  2. 48 CFR 1352.215-70 - Proposal preparation.

    Science.gov (United States)

    2010-10-01

    ... preparation. As prescribed in 48 CFR 1315.204-570(a)(1), insert the following provision, tailored as...: number of trips anticipated, number of person days, cost-per-trip-per person, destination(s) proposed... quoted daily or hourly rate. Include Consulting Agreements entered into between consultant(s) and...

  3. 48 CFR 2052.215-71 - Project officer authority.

    Science.gov (United States)

    2010-10-01

    ... performance. (4) Changes any of the expressed terms, conditions, or specifications of the contract. (5... contract is: Name: * Address: * Telephone Number: * (b) Performance of the work under this contract is... furnished to the contracting officer. (e) The contractor shall proceed promptly with the performance...

  4. Dicty_cDB: SSM215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .002 1 AU176207 |AU176207.1 Penaeus japonicus cDNA forward end of clone:PJV601. 54 0.002 1 BX323881 |BX32388...AU175995.1 Penaeus japonicus cDNA forward end of clone:PJV77. 54 0.002 1 dna update 2003. 8.12 Homology vs P

  5. 48 CFR 2152.215-70 - Contractor records retention.

    Science.gov (United States)

    2010-10-01

    ... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS... make available all records applicable to a contract term that support the annual financial report for...

  6. 48 CFR 1352.215-76 - Cost or pricing data.

    Science.gov (United States)

    2010-10-01

    ... pricing data. As prescribed in 48 CFR 1315.204-570(b)(4), insert the following provision: Cost or Pricing... Statement of Work. A brief definition of the education and experience requirements which qualify an employee....—should be discussed. (3) Overhead Costs. Generally, the offeror's accounting system and...

  7. 48 CFR 2052.215-75 - Proposal presentation and format.

    Science.gov (United States)

    2010-10-01

    ..., education, and employment history of its employees, subcontractor personnel, and consultants, through... months, the offeror shall submit a Contractor Spending Plan (CSP) as part of its cost proposal. Guidance... information as set forth below to permit the Government to make a thorough evaluation and a...

  8. 48 CFR 252.215-7002 - Cost estimating system requirements.

    Science.gov (United States)

    2010-10-01

    ... applied; (2) Produces verifiable, supportable, and documented cost estimates that are an acceptable basis... the cost estimating system to the ACO on a timely basis. (e) Estimating system deficiencies. (1) The... historical costs, and other analyses used to generate cost estimates. (b) General. The Contractor...

  9. 48 CFR 215.404-75 - Fee requirements for FFRDCs.

    Science.gov (United States)

    2010-10-01

    ... FFRDC's— (1) Proportion of retained earnings (as established under generally accepted accounting methods... funding as assessed on operating cycle cash needs; and (4) Provision for funding unreimbursed costs...

  10. Dicty_cDB: VSA215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available osome IV cosmid 9934. 46 0.15 2 AI775835 |AI775835.1 EST256935 tomato resistant, Cornell Lycopersicon escule...21334 tomato flower buds 3-8 mm, Cornell University Lycopersicon esculentum cDNA clone cTOB10G7 5', mRNA seq

  11. DNA tests for strawberry: perpetual flowering - Bx215

    Science.gov (United States)

    Perpetual flowering strawberries have great economic value to the fresh market industry. Floral initiation in strawberry is largely determined by photoperiod, temperature, and genetics. Commercially grown strawberries are generally classified as remontant (repeated or perpetual flowering, day neutr...

  12. A study of the higher excitation levels of 11B via the 10B(n,n)10B and 10B(n,n')10B*(0.72, 1.74, 2.15, 3.59, 4.77 MeV) reactions

    International Nuclear Information System (INIS)

    As part of the study of the higher energy-level structure of 11B, cross sections for elastic and inelastic scattering of neutrons from isotopically enriched 10B samples have been measured for incident neutron energies from 3.03 MeV to 6.45 MeV in 250 keV increments and from 7.02 MeV to 12.01 MeV in 500 keV increments. Inelastic angular distributions for scattering to the states in parentheses in 10B have been measured from the indicated energy up to 12.01 MeV; (0.718) from 3.02 MeV; (1.74) from 3.27 MeV; (2.15) from 3.77 MeV; (3.59) from 5.52 MeV; (4.77) from 7.02 MeV. The measurements at 3.02, 3.51, 4.02, and 4.51 MeV were done at nine laboratory angles from 20/degree/ to 158/degree/ in 17.5/degree/ increments with a sample that is isotopically 95.86% 10B. All other distributions measured scattering at 11 laboratory angles from 18/degree/ to 158/degree/ in 15/degree/ increments from a sample that is isotopically 99.49% 10B. The data are corrected for air scattering, sample attenuation, minor isotope impurity, multiple scattering, and elastic and inelastic scattering from the sample of the neutron source continuum and contaminants. An eight-channel, multilevel R-matrix analysis was performed on the data. Level energies, spins, and parities were deduced for twelve levels above 13 MeV excitation in 11B. Only two definite and three tentative assignments for T = /1/2/ levels had been made previously above 13 MeV. The two definite levels were confirmed. Good agreement between the data and the R-matrix calculation in all analyzed channels was obtained for the proposed structure. 122 refs., 40 figs., 7 tabs

  13. Study on laser atomic spectroscopy

    International Nuclear Information System (INIS)

    Laser atomic spectroscopic study on actinium element has been performed in many areas of spectroscopy. The study on characteristic of atomic vapor has been proceeded for copper atom and the spatial density distribution of copper vapor is measured. This experimental data has been compared with the theoretically calculated data. In spectroscopic experiment, the first and second excited states for actinium element are identified and the most efficient ionization scheme for actinium element is identified. In addition, the corrosion problem for filament material due to the heating of the actinium element has been studied. (Author)

  14. P2-15: EEG Analysis on Story Change in TV Drama

    Directory of Open Access Journals (Sweden)

    Chung-Yeon Lee

    2012-10-01

    Full Text Available The human brain naturally recognizes a change of environment or atmosphere without great effort, and this is essential for interactive communication in social life and a specific reaction in an emergency situation. Most studies have investigated change detection of the brain with conditional experimental paradigms rather than the performance of everyday tasks. However, naturally occurring sensory stimuli are multimodal and dynamic. In an effort to study the relationship between users' induced physiological responses and changes of environment and atmosphere under more naturalistic and ecological conditions, we performed a basic experiment using audio-visual movies and electroencephalogram (EEG measurement. 8 healthy subjects were asked to watch a television sitcom without any responses, and their EEG signals were recorded simultaneously with 126 electrodes mounted in an elastic electrode cap. Time-frequency analysis of EEG revealed distinctive neural oscillations at the point of story change in the movie. This result could be used for applications in brain-computer interfaces, and provides a reference to cognitive impairment studies such as Attention Deficit Disorder (ADD or Attention-Deficit/Hyperactivity Disorder (ADHD.

  15. 75 FR 32440 - Catalog of Federal Domestic Assistance (CFDA) Number: 84.215J

    Science.gov (United States)

    2010-06-08

    ..., and selection criteria for this program in the Federal Register on February 8, 2010 (75 FR 6188-6192... neighborhoods with increased rates of trauma due to violence, drug use, and other environmental stressors... and strong infrastructure is likely to result in the institutionalization of community...

  16. PS2-15: Coding for Obesity in a Health Plan Claims Database

    OpenAIRE

    Shainline, Michael; Carter, Shelley; Von Worley, Ann; Gunter, Margaret

    2010-01-01

    Background and Aims: The Centers for Disease Control estimated the obesity rate in New Mexico for 2008 to be 25.2%. Sources estimate the following associations between obesity and type 2 diabetes (80%); cardiovascular disease (70%); hypertension (26 %). Yet obesity is infrequently coded as a secondary diagnosis among providers submitting claims. This study examines the frequency with which obesity is documented on claims forms, the relationship between age, gender, and obesity coding, and the...

  17. 7 CFR 215.13a - Determining eligibility for free milk in child-care institutions.

    Science.gov (United States)

    2010-01-01

    ... AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL MILK PROGRAM FOR.... Russell National School Lunch Act or the Child Nutrition Act of 1966. This means that all eligibility... the Child Nutrition Act of 1966 or the Richard B. Russell National School Lunch Act. (i)...

  18. Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs

    OpenAIRE

    Dunham Rex; Muir William; Liu Lei; Turan Cemal; Simmons Micah; Serapion Jerry; Somridhivej Benjaporn; Nandi Samiran; Kucuktas Huseyin; Xu Peng; Baoprasertkul Puttharat; He Chongbo; Feng Jinian; Wang Shaolin; Peatman Eric

    2007-01-01

    Abstract Background EST sequencing is one of the most efficient means for gene discovery and molecular marker development, and can be additionally utilized in both comparative genome analysis and evaluation of gene duplications. While much progress has been made in catfish genomics, large-scale EST resources have been lacking. The objectives of this project were to construct primary cDNA libraries, to conduct initial EST sequencing to generate catfish EST resources, and to obtain baseline inf...

  19. 48 CFR 52.215-2 - Audit and Records-Negotiation.

    Science.gov (United States)

    2010-10-01

    ... ordinary course of business or pursuant to a provision of law. (e) Reports. If the Contractor is required... (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 52... are in written form, in the form of computer data, or in any other form. (b) Examination of costs....

  20. 48 CFR 215.407-5-70 - Disclosure, maintenance, and review requirements.

    Science.gov (United States)

    2010-10-01

    ... management systems (e.g., production control or cost accounting) with the estimating system so that the...) Using additional cost analysis techniques to determine the reasonableness of the cost elements...

  1. 9 CFR 381.215 - Poultry or other articles subject to judicial seizure and condemnation.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Poultry or other articles subject to... INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION POULTRY PRODUCTS INSPECTION...

  2. ICA based automatic segmentation of dynamic H(2)(15)O cardiac PET images.

    Science.gov (United States)

    Margadán-Méndez, Margarita; Juslin, Anu; Nesterov, Sergey V; Kalliokoski, Kari; Knuuti, Juhani; Ruotsalainen, Ulla

    2010-05-01

    In this study, we applied an iterative independent component analysis (ICA) method for the separation of cardiac tissue components (myocardium, right, and left ventricle) from dynamic positron emission tomography (PET) images. Previous phantom and animal studies have shown that ICA separation extracts the cardiac structures accurately. Our goal in this study was to investigate the methodology with human studies. The ICA separated cardiac structures were used to calculate the myocardial perfusion in two different cases: 1) the regions of interest were drawn manually on the ICA separated component images and 2) the volumes of interest (VOI) were automatically segmented from the component images. For the whole myocardium, the perfusion values of 25 rest and six drug-induced stress studies obtained with these methods were compared to the values from the manually drawn regions of interest on differential images. The separation of the rest and stress studies using ICA-based methods was successful in all cases. The visualization of the cardiac structures from H (2) (15) O PET studies was improved with the ICA separation. Also, the automatic segmentation of the VOI seemed to be feasible. PMID:19273031

  3. 14 CFR 1274.215 - Federal and federally funded construction projects.

    Science.gov (United States)

    2010-01-01

    ... Administrator for Procurement may exempt a construction project from this policy if, as of February 17, 2001— (1... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Federal and federally funded construction... construction projects. (a) In accordance with E.O. 13202 of February 17, 2001, “Preservation of...

  4. 29 CFR 98.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-07-01

    ... responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security Income provisions of the...

  5. 2 CFR 180.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-01-01

    ... individual's present responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security Income provisions of the Social Security Act, 42 U.S.C. 1301 et seq., those benefits are not...

  6. 7 CFR 3017.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-01-01

    ... responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security Income provisions of the...

  7. 41 CFR 105-68.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-07-01

    ... an individual as a personal entitlement without regard to the individual's present responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security Income provisions of the Social...

  8. 34 CFR 85.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-07-01

    ... individual's present responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security...: E.O. 12549 (3 CFR, 1986 Comp., p. 189); E.O 12689 (3 CFR, 1989 Comp., p. 235); 20 U.S.C. 1082,...

  9. 48 CFR 1552.215-73 - General financial and organizational information.

    Science.gov (United States)

    2010-10-01

    ... Number: (d) Individual(s) to contact re. this proposal: (e) Cognizant Government: Audit Agency: Address...-reimbursement type prime contracts and subcontracts $____ Government fixed-price prime contracts and... prime contracts, subcontractors under Government prime contracts, and modifications (except when...

  10. 40 CFR 80.215 - What is the scope of the geographic phase-in program?

    Science.gov (United States)

    2010-07-01

    ... area. (1) The following states comprise the geographic phase-in area (GPA) subject to the provisions of... are included in the GPA: (i) The list of counties follows: Arizona Apache Coconino Gila Greenlee... reservations of a particular tribe are included in the GPA if a portion of the tribal reservation is within...

  11. 22 CFR 1006.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-04-01

    ... international organization; (3) An entity owned (in whole or in part) or controlled by a foreign government; or... or disaster. (e) A permit, license, certificate, or similar instrument issued as a means to...

  12. 22 CFR 1508.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-04-01

    ... international organization; (3) An entity owned (in whole or in part) or controlled by a foreign government; or... emergency or disaster. (e) A permit, license, certificate, or similar instrument issued as a means...

  13. Hyperspectral Imagery for the Main Eight Hawaiian Islands:Oahu (215-0611-272217)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort among the National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment; the...

  14. 47 CFR 73.215 - Contour protection for short-spaced assignments.

    Science.gov (United States)

    2010-10-01

    ... station pursuant to 47 CFR 73.211(b)(3): 6 kW ERP/240 meters HAAT—Class A 25 kW ERP/150 meters HAAT—Class B1 50 kW ERP/472 meters HAAT—Class B (b) Applicants requesting short-spaced assignments pursuant to... achieved: (1) The ERP and antenna HAAT of the proposed station in the direction of the contours of...

  15. Self-biased 215 MHz magnetoelectric NEMS resonator for ultra-sensitive DC magnetic field detection.

    Science.gov (United States)

    Nan, Tianxiang; Hui, Yu; Rinaldi, Matteo; Sun, Nian X

    2013-01-01

    High sensitivity magnetoelectric sensors with their electromechanical resonance frequencies DC magnetic fields. This magnetoelectric NEMS resonator showed a high quality factor of 735, and strong magnetoelectric coupling with a large voltage tunable sensitivity. The admittance of the magnetoelectric NEMS resonator was very sensitive to DC magnetic fields at its electromechanical resonance, which led to a new detection mechanism for ultra-sensitive self-biased RF NEMS magnetoelectric sensor with a low limit of detection of DC magnetic fields of ~300 picoTelsa. The magnetic/piezoelectric heterostructure based RF NEMS magnetoelectric sensor is compact, power efficient and readily integrated with CMOS technology, which represents a new class of ultra-sensitive magnetometers for DC and low frequency AC magnetic fields. PMID:23760520

  16. 42 CFR 405.215 - Confidential commercial and trade secret information.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false Confidential commercial and trade secret... HEALTH AND HUMAN SERVICES MEDICARE PROGRAM FEDERAL HEALTH INSURANCE FOR THE AGED AND DISABLED Medical... trade secret information. To the extent that CMS relies on confidential commercial or trade...

  17. 48 CFR 1552.215-72 - Instructions for the Preparation of Proposals.

    Science.gov (United States)

    2010-10-01

    .... These hours do not include management at a level higher than project management, e.g., corporate and day-to-day management, nor do they include clerical and support staff at a level lower than technician.... State the amount of service estimated to be required and the quoted daily or hourly rate. Offerors...

  18. 48 CFR 52.215-1 - Instructions to Offerors-Competitive Acquisition.

    Science.gov (United States)

    2010-10-01

    ... withdrawal of proposals. (1) Unless other methods (e.g., electronic commerce or facsimile) are permitted in... transmitted through an electronic commerce method authorized by the solicitation, it was received at the... numbers of the offeror (and electronic address if available); (iii) A statement specifying the extent...

  19. TRANSX-2.15, Neutron Gamma Particle Transport Tables from MATXS Format Cross-Sections

    International Nuclear Information System (INIS)

    1 - Description of program or function: TRANSX is a computer code that reads nuclear data from a library in MATXS format and produces transport tables compatible with many discrete-ordinates (SN) and diffusion codes. Tables can be produced for neutron, photon, charged-particle, or coupled transport. Options include adjoint tables, mixtures, homogeneous or heterogeneous self-shielding, group collapse, homogenization, thermal up-scatter, prompt or steady-state fission, transport corrections, elastic removal corrections, and flexible response function edits. 2 - Method of solution: TRANSX reads through the materials in a MATXS library and accumulates the cross sections into a transport table using the user's mix instructions. At the same time, response function edit cross sections are accumulated using the user's edit instructions. They can thus be any linear combination of the cross sections available in the library. When the table is complete, it is written out in the desired format. Output options include DTF-style card images, FIDO, ISOTXS, and the binary group-ordered GOXS format. Self-shielding is handled using the background cross section method. Heterogeneity options include homogeneous mixtures, escape using mean chord, lattices of cylinders by the Bell or Sauer approximations, and reflected or periodic slab cell by the bell or E3 approximations. 3 - Restrictions on the complexity of the problem: Only narrow- resonance self-shielding is available in this version. This may affect accuracy for thermal problems

  20. 48 CFR 215.404-71-3 - Contract type risk and working capital adjustment.

    Science.gov (United States)

    2010-10-01

    ... normal value when the contract type risk is low. Indicators of this are— (i) Very mature product line... paragraph (c) of this subsection using the evaluation criteria in paragraph (d) of this subsection. (2... provisions for performance-based payments, do not compute a working capital adjustment. (d)...

  1. 32 CFR 215.9 - Providing military resources to civil authorities.

    Science.gov (United States)

    2010-07-01

    ... requests for firefighting assistance to DSA. (4) Request from civil law enforcement agencies for training... 32 National Defense 2 2010-07-01 2010-07-01 false Providing military resources to civil... DEFENSE (CONTINUED) MISCELLANEOUS EMPLOYMENT OF MILITARY RESOURCES IN THE EVENT OF CIVIL...

  2. 40 CFR 94.215 - Maintenance of records; submittal of information; right of entry.

    Science.gov (United States)

    2010-07-01

    ... family for which the manufacturer fails to retain the records required in this section or to provide such... material not translated into the English language need not be submitted unless specifically requested by... influence exhaust emissions will be representative of production engines and that either: all components...

  3. 31 CFR 19.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-07-01

    ... entitlement without regard to the individual's present responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security Income provisions of the Social Security Act, 42 U.S.C. 1301 et...

  4. 22 CFR 208.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-04-01

    ... individual's present responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security Income provisions of the Social Security Act, 42 U.S.C. 1301 et seq., those benefits are not...

  5. 5 CFR 919.215 - Which nonprocurement transactions are not covered transactions?

    Science.gov (United States)

    2010-01-01

    ... an individual as a personal entitlement without regard to the individual's present responsibility (but benefits received in an individual's business capacity are not excepted). For example, if a person receives social security benefits under the Supplemental Security Income provisions of the Social...

  6. 48 CFR 52.215-10 - Price Reduction for Defective Certified Cost or Pricing Data.

    Science.gov (United States)

    2010-10-01

    ... complete, accurate, and current as certified in its Certificate of Current Cost or Pricing Data; (2) A... complete, accurate, and current as certified in the Contractor's Certificate of Current Cost or Pricing... been modified even if accurate, complete, and current certified cost or pricing data had been...

  7. 33 CFR 106.215 - Company or OCS facility personnel with security duties.

    Science.gov (United States)

    2010-07-01

    ... screenings of persons, personal effects, stores and industrial supplies; (k) Familiarity with all relevant... appropriate: (a) Knowledge of current and anticipated security threats and patterns. (b) Recognition...

  8. 48 CFR 1452.215-71 - Use and Disclosure of Proposal Information-Department of the Interior.

    Science.gov (United States)

    2010-10-01

    ...) “Trade Secret” means an unpatented, secret, commercially valuable plan, appliance, formula, or process... trade commodities. (2) “Confidential commercial or financial information” means any business information... Information Act, 5 U.S.C. 552. Exemptions from mandatory disclosure which may be applicable to...

  9. Oahu Hyperspectral Imagery 2000 (215-0611-332211) - Visual Interpretation from Remote Sensing Imagery Main Eight Hawaiian Islands

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort between the National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment,...

  10. Equilibrium geometries and electronic properties of BenLi(n=2-15)clusters from first principles

    Institute of Scientific and Technical Information of China (English)

    Lei Xue-Ling; Zhu Heng-Jiang; Wang Xian-Ming; Luo You-Hua

    2008-01-01

    This paper studies the equilibrium geometries and electronic properties of Ben and BenLi clusters,up to n=15,by using density-functional theory(DFT)at B3LYP/6-31G(d)level.The lowest-energy structures of Ben and BenLi clusters were determined.The results indicate that a single lithium impurity enhances the stability and chemical reactivity of the beryllium clusters.It finds that the geometries of the host clusters change significantly after the addition of the lithium atom for n≥8.The lithium impurity prefers to be on the periphery of beryllium clusters,and occupies vertex sites.Both Be4Li,Be9Li,and Be13Li were found to be particularly stable with higher average binding energy,local peaks of second-order energy difference and fragmentation energies.For all the BenLi clusters studied,we found charge transfers from the Li to Be site and co-existence of covalent and metallic bonding characteristics.

  11. 8 CFR 215.8 - Requirements for biometric identifiers from aliens on departure from the United States.

    Science.gov (United States)

    2010-01-01

    ... attendants, servants, or personal employees of accredited officials), G-1, G-2, G-3, G-4, NATO-1, NATO-2, NATO-3, NATO-4, NATO-5, or NATO-6 visas, and certain Taiwan officials who hold E-1 visas and members...

  12. SU-E-J-215: Onboard Split PET Including the Effects of Attenuation, Scatter, and Random Events

    International Nuclear Information System (INIS)

    Purpose: Positron emission tomography (PET) using split PET geometry was investigated as an on-board system for functional imaging and PET marker tracking, specifically with tomotherapy. The open dual ring PET would allow measurement of both inter and intra-fractional variation, improving the delineation of tumor volume at any stage in the radiation treatment delivery process. We present results from data obtained using Monte Carlo simulations including the effects of attenuation, random events, and scatter. Methods: PET design was accomplished via Monte Carlo simulations with GATE, the Geant4 Application for Emission Tomography. Images were reconstructed using Software for Image Reconstruction (STIR) with a fully 3D ordered subset expectation maximization (OSEM) image reconstruction technique. Results: Monte Carlo simulations of the split PET geometry indicate that including physical factors that degrade image quality such as attenuation, random events, and scatter still prove the feasibility of near real-time PET for both inter and intra-fractional radiation delivery. The image quality of scan times under 1 minute reveals that it is possible to utilize PET scanning and reconstruction during the treatment session intrafractionally. GATE also simulates the depth information but does not correct obliqueness of the path of line of response so the data is much more realistic than the data obtained with ray tracing. Conclusion: Onboard split PET with TomoTherapy can generate quality images under 1 minute scan times without the need to correct for attenuation, scatter, random events, or depth information of the interaction

  13. 75 FR 17156 - Gulf of Mexico, Outer Continental Shelf, Western Planning Area, Oil and Gas Lease Sale 215 (2010...

    Science.gov (United States)

    2010-04-05

    ... 1.4-nautical-mile buffer zone north of the OCS boundary between the U.S. and Mexico) based on... Consistency Determination (CD) to determine whether the lease sale is consistent with each affected State's... of Sale (NOS) from the governors of the affected States on the size, timing, and location of...

  14. 12 CFR Appendix to Part 215 - Section 5200 of the Revised Statutes Total Loans and Extensions of Credit

    Science.gov (United States)

    2010-01-01

    ...— (1) The term loans and extensions of credit shall include all direct or indirect advances of funds to... the Currency, such term shall also include any liability of a national banking association to advance... discount by dealers in dairy cattle of paper given in payment for dairy cattle, which paper carries a...

  15. 20 CFR 645.215 - What must a WtW operating entity that serves noncustodial parent participants do?

    Science.gov (United States)

    2010-04-01

    ... and children who may be at risk of domestic violence, the operating entity must consult with domestic violence prevention and intervention organizations in the development of its WtW project serving... that good cause exists to extend this period. This extension may not extend to a date more than...

  16. 16 CFR 2.15 - Orders requiring witnesses to testify or provide other information and granting immunity.

    Science.gov (United States)

    2010-01-01

    ... Bureaus of Competition and Economics, the Bureau Director, Deputy Directors and Associate Directors of the... essential to serve the interests of justice. Such appeals shall be made on the record and shall be in...

  17. The Arizona Radio Observatory 1 mm Spectral Survey of IRC (plus)10216 and VY Canis Majoris (215-285 GHz)

    Science.gov (United States)

    Tenenbaum, E. D.; Dodd, J. L.; Milam, S. N.; Woolf, N. J.; Ziurys, L. M.

    2010-01-01

    A low noise (1(sigma) rms approx. 3 mK) 1. nun spectral survey (214.5-285.5 GHz) of the oxygen-rich supergiant VY Canis Majoris and the carbon-rich asymptotic giant branch star IRC +10216 has been conducted using the Arizona Radio Observatory's 10 m Submillimeter Telescope. Here the complete data set is presented. This study, carried out with a new ALMA-type receiver, marks the first continuous band scan of an O-rich circumstellar envelope, and the most sensitive survey to date of IRC +10216. In VY CMa, 130 distinct molecular lines were detected, 14 of which cannot be identified; in IRC +10216, 717 lines were observed, with 126 features remaining unidentified. In the 1 mm bands of VY CMa and IRC +10216, emission is present from 18 and 32 different chemical compounds, respectively, with 10 species common to both sources. Many narrow emission lines were observed in both circumstellar shells, arising from vibrationally excited molecules and from refractory-containing species. Line profiles in VY CMa also exhibit a variety of different shapes, caused by the complex, asymmetric outflow of this object. The survey highlights the fact that C-rich and O-rich circumstellar envelopes are chemically interesting, and both are sources of new interstellar molecules. The high number of unidentified lines and the unreliable, rest frequencies for known species such as NaCN indicate the need for additional laboratory spectroscopy studies.

  18. “Remotion” Total Wrist Arthroplasty: Preliminary Results of a Prospective International Multicenter Study of 215 Cases

    DEFF Research Database (Denmark)

    Herzberg, Guillaume; Boeckstyns, Michel Ernest Henri; Sorensen, Allan Ibsen;

    2012-01-01

    This study reports the current results of an international multicenter study of one last generation total wrist arthroplasty (TWA) ("ReMotion," Small Bone Innovation, Morristown, PA). The two first authors (G.H. and M.B.) built a Web-based prospective database including clinical and radiological ...

  19. Oahu Hyperspectral Imagery 2000 (215-0611-272217) - Visual Interpretation from Remote Sensing Imagery Main Eight Hawaiian Islands

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort between the National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment,...

  20. Structure and torsional dynamics of the water octamer from THz laser spectroscopy near 215 μm.

    Science.gov (United States)

    Cole, William T S; Farrell, James D; Wales, David J; Saykally, Richard J

    2016-06-01

    Clusters of eight water molecules play an important role in theoretical analysis of aqueous structure and dynamics but have proven to be challenging experimental targets. Here we report the high-resolution spectroscopic characterization of the water octamer. Terahertz (THz) vibration-rotation-tunneling (VRT) spectroscopy resolved 99 transitions with 1 part per million precision in a narrow range near 46.5 wave numbers, which were assigned to the h16 octamer via detailed isotope dilution experiments. Fitting to a semi-rigid symmetric top model supports predictions of two coexisting cuboidal structures and provides precise values for the changes in their rotational constants. Comparison with theory and previous spectroscopic data provides a characterization of the two structures and the observed torsional vibration and supports the prediction that the D2d symmetry structure is lower in energy than the S4 isomer. PMID:27257252

  1. 48 CFR 1652.215-70 - Rate Reduction for Defective Pricing or Defective Cost or Pricing Data.

    Science.gov (United States)

    2010-10-01

    ... that were not complete, accurate, or current as certified in the Certificate of Accurate Cost or... accurate, complete, and current cost or pricing data had been submitted or maintained and identified. (ii... submit or keep in its files a Certificate of Current Cost or Pricing Data. (2)(i) Except as prohibited...

  2. 48 CFR 52.215-11 - Price Reduction for Defective Certified Cost or Pricing Data-Modifications.

    Science.gov (United States)

    2010-10-01

    ... subcontractor furnished certified cost or pricing data that were not complete, accurate, and current as certified in its Certificate of Current Cost or Pricing Data, (2) a subcontractor or prospective... current as certified in the Contractor's Certificate of Current Cost or Pricing Data, or (3) any of...

  3. Regional cerebral blood flow during light sleep--a H(2)(15)O-PET study

    DEFF Research Database (Denmark)

    Kjaer, Troels W; Law, Ian; Wiltschiøtz, Gordon;

    2002-01-01

    to other forms of altered awareness, for example, relaxation meditation than to deeper sleep stages. We are of the opinion that stage-1 sleep represents the dreaming state of wakefulness, while rapid eye movement (REM) sleep reflects the dreaming state of the unaware, sleeping brain.......This is the first report on the distribution of regional cerebral blood flow (rCBF) changes during stage-1 sleep or somnolence. Two hypotheses were tested: (A) that rCBF differed between the awake relaxed state and stage-1 sleep, (B) that hypnagogic hallucinations frequently experienced at sleep...... onset would be accompanied by measurable changes in rCBF using positron emission tomography (PET). Eight subjects were PET-scanned with (15)O-labeled water injection in three conditions: awake, stage-1 sleep with reportable experiences and stage-1 sleep without reportable experiences...

  4. Use of gamma-ray spectrometry for analysis of Uranium isotopic composition in soil of Iran

    International Nuclear Information System (INIS)

    The use of depleted uranium (DU) in various weaponry and ammunition during the Iraq war in April, 2003 caused serious concern in Iran over possible uranium contamination of the Iran environment and consequently long health effect. After a shell explosion, uranium is discharged by the fire in the air in the form of oxidized particles and can be dispersed within a radius of several kilometers. Gamma-ray spectrometry was used to determine uranium concentration in soil samples collected from 8 sites in Iranian sectors of Iraq border. All soil samples were dried ,gently grounded and passed through a 2 mm sieve. Three hundred grams of each sample were placed in plastic container and sealed for at least 20 days to allow equilibrium in uranium, thorium and actinium series. Gamma-ray intensities were measured with 40% HPGe (CANBERRA) detector. The detector was shielded by 10 cm lead on all sides with cadmium-copper in inner sides. The system is equipped with software for data acquisition and analyzing. The counting time was 6x104 seconds and background spectra were also collected for the same period of time. The concentrations of 238U assessed from 63.3 keV and 92.4 keV emission of its first daughter nuclide, 234Th. To assess the isotopic ratio of 238U/235 U, secular equilibrium was ensured and the concentration of 235U under the 186 keV was deduced. The 226Ra was determined through the 295 keV and 352 keV gamma-rays of 214Pb. The concentrations of 238U and activity ratio of 238U/235U is given. The average of measurement activity ratio is 21,very close to the value of 21.5 for natural uranium, while the activity ratio of DU can be as high as 76.9. The 238U activity ranges within typically accepted levels from 14-33 Bq kg-1, while the typical range given by UNSCEAR (1988) for different soil samples is 10 to 50 Bq kg-1. The analysis of eight surface soil samples of Iranian sites of Iraq border, showed that uranium isotopes are in their natural abundances

  5. Systemic Delivery of Small Interfering RNA Targeting the Interleukin-2/15 Receptor β Chain Prevents Disease Progression in Experimental Arthritis

    OpenAIRE

    Tiantian Zhang; Xuehua Bai; Xiaohua Mao

    2013-01-01

    The role of interleukin (IL)-15 in the pathogenesis of rheumatoid arthritis (RA) is well established; however, systemic knockdown of IL-15 receptor (IL-15R) for reduction in inflammation at local sites has not been demonstrated. In this study, the therapeutic effect of intravenously administered siRNA targeting the β chain of IL-15R which is shared by the receptor for IL-2 was examined in rats with adjuvant-induced arthritis (AA). Polyethylenimine (PEI)-complexed siRNA nanoparticles could eas...

  6. SU-E-J-215: Towards MR-Only Image Guided Identification of Calcifications and Brachytherapy Seeds: Application to Prostate and Breast LDR Implant Dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Elzibak, A; Fatemi-Ardekani, A; Soliman, A; Mashouf, S; Safigholi, H; Ravi, A; Morton, G; Song, WY [Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario (Canada); Han, D [Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario (Canada); University of California, San Diego, La Jolla, CA (United States)

    2015-06-15

    Purpose: To identify and analyze the appearance of calcifications and brachytherapy seeds on magnitude and phase MRI images and to investigate whether they can be distinguished from each other on corrected phase images for application to prostate and breast low dose rate (LDR) implant dosimetry. Methods: An agar-based gel phantom containing two LDR brachytherapy seeds (Advantage Pd-103, IsoAid, 0.8mm diameter, 4.5mm length) and two spherical calcifications (large: 7mm diameter and small: 4mm diameter) was constructed and imaged on a 3T Philips MR scanner using a 16-channel head coil and a susceptibility weighted imaging (SWI) sequence (2mm slices, 320mm FOV, TR/ TE= 26.5/5.3ms, 15 degree flip angle). The phase images were unwrapped and corrected using a 32×32, 2D Hanning high pass filter to remove background phase noise. Appearance of the seeds and calcifications was assessed visually and quantitatively using Osirix (http://www.osirix-viewer.com/). Results: As expected, calcifications and brachytherapy seeds appeared dark (hypointense) relative to the surrounding gel on the magnitude MRI images. The diameter of each seed without the surrounding artifact was measured to be 0.1 cm on the magnitude image, while diameters of 0.79 and 0.37 cm were measured for the larger and smaller calcifications, respectively. On the corrected phase images, the brachytherapy seeds and the calcifications appeared bright (hyperintense). The diameter of the seeds was larger on the phase images (0.17 cm) likely due to the dipole effect. Conclusion: MRI has the best soft tissue contrast for accurate organ delineation leading to most accurate implant dosimetry. This work demonstrated that phase images can potentially be useful in identifying brachytherapy seeds and calcifications in the prostate and breast due to their bright appearance, which helps in their visualization and quantification for accurate dosimetry using MR-only. Future work includes optimizing phase filters to best identify and delineate seeds and calcifications.

  7. Comparative Spectra of Oxygen-Rich Versus Carbon-Rich Circumstellar Shells: VY Canis Majoris and IRC(plus)10216 at 215-285 GHz

    Science.gov (United States)

    Tenebaum, E. D.; Dodd, J. L.; Milam, S. N.; Woolf, N. J.; Ziurys, L. M.

    2010-01-01

    A sensitive (1sigma rms at 1 MHz resolution approx.3 mK) 1 mm spectral line survey (214.5-285.5 GHz) of VY Canis Majoris (VY CMa) and IRC +10216 has been conducted to compare the chemistries of oxygen- and carbon-rich circumstellar envelopes. This study was carried out using the Submillimeter Telescope of the Arizona Radio Observatory with a new Atacama Large Millimeter Array type receiver. This survey is the first to chemically characterize an O-rich circumstellar shell at millimeter wavelengths. In VY CMa, 128 emission features were detected arising from 18 different molecules; and in IRC +10216, 720 lines were observed, assigned to 32 different species. The 1 mm spectrum of VY CMa is dominated by SO, and SiS; in IRC +10216, C4H and SiC2 are the most recurrent species. Ten molecules were common to both sources: CO, SiS, SiO, CS, CN, HCN, HNC, NaCl, PN, and HCO(+). Sulfur plays an important role in VY CMa, but saturated/ unsaturated carbon dominates the molecular content of IRC +102.16, producing CH2NH, for example. Although the molecular complexity of IRC +10216 is greater, VY CMa supports a unique "inorganic" chemistry leading to the oxides PO, AlO, and AlOH. Only diatomic and triatomic compounds were observed in VY CMa, while species with four or more atoms are common in IRC +10216, reflecting carbon's ability to form multiple strong bonds, unlike oxygen. In VY CMa, a new water maser (v2 = 2) has been found, as well as vibrationally excited NaCl. Toward IRC +10216, vibrationally excited CCH was detected for the first time.

  8. 77 FR 20408 - [FWS-R9-MB-2011-N215; 91400-5110-0000-7B; 91400-9410-0000-7B

    Science.gov (United States)

    2012-04-04

    ... nongovernmental organizations that represent conservation organizations, sportsmen's and women's organizations... Partnerships to Increase Board. Capacity. 12-021........ Trailblazer Adventure U.S. Sportsman 40,000.00...

  9. SOLVENT HOLD TANK SAMPLE RESULTS FOR MCU-14-135/136 AND MCU-14-214/215/216: FEBRUARY AND MARCH 2014 MONTHLY SAMPLES

    Energy Technology Data Exchange (ETDEWEB)

    Fondeur, F.; Taylor-Pashow, K.

    2014-06-30

    SRNL received two sets of SHT samples (MCU-14-135-136 in February 2014 and MCU-14-214-216 in March 2014) for analysis. The samples were analyzed for composition. As with the previous solvent sample results, these analyses indicate that the solvent does not require Isopar® L trimming at this time. However, the addition of TiDG (suppressor) to the blended solvent is recommended. Evidence of possible (slight) isomerization of the solvent, probably Isopar®L or TiDG degradation products, was observed.

  10. Electromotive force generation using the dynamic response of Ni0Mn28.5Ga21.5 magnetic shape memory alloy

    Science.gov (United States)

    Bruno, N.; Ciocanel, C.; Feigenbaum, H.

    2011-04-01

    Magnetic Shape Memory Alloys (MSMAs) are materials that respond to a change in either compressive stress or magnetic field, and can be used for applications such as actuation, sensing, and power harvesting. MSMA prismatic specimens are usually loaded magneto-mechanically by a compressive stress applied along the longest side of the specimen and by a magnetic field applied normal to the direction of the compressive stress. Karaman et al. proved the viability of using MSMAs, specifically NiMnGa single crystals, for energy harvesting applications using up to 5 Hz of cyclic stress. The group proposed a simple mathematical model to predict electrical voltage output generated by the material during the shape recovery process. The voltage output predicted by the model matched well with experimental results recorded at low frequencies1. The magnetization reversal responsible for the voltage output has been approximated by Karaman et al. does not use the constitutive relations for the magneto-mechanical behavior of the material, such as that proposed by Kiefer and Lagoudas2,3. This work presents simulated and experimental results describing the electromotive force (EMF) producing capabilities of a NiMnGa magnetic shape memory alloy (MSMA) at frequencies of up to 10 Hz. Unlike previous work, the current paper uses the constitutive model developed by Kiefer and Lagoudas2-4 and the corresponding magnetization relations to theoretically predict the voltage output of the material. COMSOL Multiphysics 3.5a and Simulink were used to generate the simulated results for different constant bias magnetic fields and frequencies of excitation, partial reorientation strains and stress amplitudes. Simulated results are compared to experimental data and the reasons for data match/mismatch are discussed.

  11. Comparative Spectra of Oxygen-Rich vs. Carbon-Rich Circumstellar Shells: VY Canis Majoris and IRC+10216 at 215-285 GHz

    CERN Document Server

    Tenenbaum, E D; Milam, S N; Woolf, N J; Ziurys, L M

    2010-01-01

    A sensitive (1{\\sigma} rms at 1 MHz resolution ~3 mK) 1 mm spectral line survey (214.5-285.5 GHz) of VY Canis Majoris (VY CMa) and IRC+10216 has been conducted to compare the chemistries of oxygen and carbon-rich circumstellar envelopes. This study was carried out using the Submillimeter Telescope (SMT) of the Arizona Radio Observatory (ARO) with a new ALMA-type receiver. This survey is the first to chemically characterize an O-rich circumstellar shell at millimeter wavelengths. In VY CMa, 128 emission features were detected arising from 18 different molecules, and in IRC+10216, 720 lines were observed, assigned to 32 different species. The 1 mm spectrum of VY CMa is dominated by SO2 and SiS; in IRC +10216, C4H and SiC2 are the most recurrent species. Ten molecules were common to both sources: CO, SiS, SiO, CS, CN, HCN, HNC, NaCl, PN, and HCO+. Sulfur plays an important role in VY CMa, but saturated/unsaturated carbon dominates the molecular content of IRC+10216, producing CH2NH, for example. Although the mol...

  12. Thrombin mutant W215A/E217A treatment improves neurological outcome and attenuates central nervous system damage in experimental autoimmune encephalomyelitis.

    Science.gov (United States)

    Verbout, Norah G; Yu, Xiaolin; Healy, Laura D; Phillips, Kevin G; Tucker, Erik I; Gruber, András; McCarty, Owen J T; Offner, Halina

    2015-02-01

    Multiple sclerosis (MS) is a neuroinflammatory disease characterized by demyelination and axonal damage of the central nervous system. The pathogenesis of MS has also been linked to vascular inflammation and local activation of the coagulation system, resulting in perivascular fibrin deposition. Treatment of experimental autoimmune encephalomyelitis (EAE), a model of human MS, with antithrombotic and antiinflammatory activated protein C (APC) reduces disease severity. Since recombinant APC (Drotecogin alfa), originally approved for the treatment of severe sepsis, is not available for human MS studies, we tested the hypothesis that pharmacologic activation of endogenous protein C could likewise improve the outcome of EAE. Mice were immunized with murine myelin oligodendrocyte glycoprotein (MOG) peptides and at the onset of EAE symptoms, were treated every other day with either WE thrombin (25 μg/kg; i.v.), a selective recombinant protein C activator thrombin analog, or saline control. Mice were monitored for changes in disease score until euthanized for ex vivo analysis of inflammation. Administration of WE thrombin significantly ameliorated clinical severity of EAE, reduced inflammatory cell infiltration and demyelination, suppressed the activation of macrophages comprising the CD11b + population and reduced accumulation of fibrin (ogen) in the spinal cord. These data suggest that symptomatic MS may respond to a treatment strategy that involves temporal pharmacological enhancement of endogenous APC generation. PMID:24810631

  13. The plant cytoskeleton: recent advances in the study of the plant microtubule-associated proteins MAP-65, MAP-190 and the Xenopus MAP215-like protein, MOR1.

    Science.gov (United States)

    Hussey, Patrick J; Hawkins, Timothy J; Igarashi, Hisako; Kaloriti, Despina; Smertenko, Andrei

    2002-12-01

    The microtubule cytoskeleton is a dynamic filamentous structure involved in many key processes in plant cell morphogenesis including nuclear and cell division, deposition of cell wall, cell expansion, organelle movement and secretion. The principal microtubule protein is tubulin, which associates to form the wall of the tubule. In addition, various associated proteins bind microtubules either to anchor, cross-link or regulate the microtubule network within cells. Biochemical, molecular biological and genetic approaches are being successfully used to identify these microtubule-associated proteins (MAPs) in plants, and we describe recent progress on three of these proteins. PMID:12516862

  14. Phylogeographic Analysis of Haplogroup E3b (E-M215) Y Chromosomes Reveals Multiple Migratory Events Within and Out Of Africa

    Science.gov (United States)

    Cruciani, Fulvio; La Fratta, Roberta; Santolamazza, Piero; Sellitto, Daniele; Pascone, Roberto; Moral, Pedro; Watson, Elizabeth; Guida, Valentina; Colomb, Eliane Beraud; Zaharova, Boriana; Lavinha, João; Vona, Giuseppe; Aman, Rashid; Calì, Francesco; Akar, Nejat; Richards, Martin; Torroni, Antonio; Novelletto, Andrea; Scozzari, Rosaria

    2004-01-01

    We explored the phylogeography of human Y-chromosomal haplogroup E3b by analyzing 3,401 individuals from five continents. Our data refine the phylogeny of the entire haplogroup, which appears as a collection of lineages with very different evolutionary histories, and reveal signatures of several distinct processes of migrations and/or recurrent gene flow that occurred in Africa and western Eurasia over the past 25,000 years. In Europe, the overall frequency pattern of haplogroup E-M78 does not support the hypothesis of a uniform spread of people from a single parental Near Eastern population. The distribution of E-M81 chromosomes in Africa closely matches the present area of distribution of Berber-speaking populations on the continent, suggesting a close haplogroup–ethnic group parallelism. E-M34 chromosomes were more likely introduced in Ethiopia from the Near East. In conclusion, the present study shows that earlier work based on fewer Y-chromosome markers led to rather simple historical interpretations and highlights the fact that many population-genetic analyses are not robust to a poorly resolved phylogeny. PMID:15042509

  15. 10 CFR Appendix E to Part 20 - Nationally Tracked Source Thresholds

    Science.gov (United States)

    2010-01-01

    ... Category 1(TBq) Category 1(Ci) Category 2(TBq) Category 2(Ci) Actinium-227 20 540 0.2 5.4 Americium-241 60... 2 54 Strontium-90 1,000 27,000 10 270 Thorium-228 20 540 0.2 5.4 Thorium-229 20 540 0.2 5.4...

  16. I. The metabolic properties of plutonium and allied materials

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, J.G.

    1948-05-24

    This report on the metabolic properties of plutonium and related radioactive materials presents experimental information in the following areas: radioautographic studies; tracer studies (with tables of accumulation in tissues) of actinium, radio-zirconium, technetium, radio-rubidium, radio-germanium, beryllium, and cadmium; decontamination and bone metabolism studies; and radio-chemical isolation.

  17. 10 CFR Appendix B to Part 20 - Annual Limits on Intake (ALIs) and Derived Air Concentrations (DACs) of Radionuclides for...

    Science.gov (United States)

    2010-01-01

    .... Actinium Ac 89 Aluminum Al 13 Americium Am 95 Antimony Sb 51 Argon Ar 18 Arsenic As 33 Astatine At 85... Curium Cm 96 Dysprosium Dy 66 Einsteinium Es 99 Erbium Er 68 Europium Eu 63 Fermium Fm 100 Fluorine F...

  18. Efeito da implantação de rodovias no cerrado brasileiro sobre a fauna de vertebrados - DOI: 10.4025/actascibiolsci.v28i3.215 Effect of the implantation of roads in the brazilian cerrado on the fauna of vertebrates - DOI: 10.4025/actascibiolsci.v28i3.215

    Directory of Open Access Journals (Sweden)

    Zara Faria Sobrinha Guimarães

    2007-11-01

    Full Text Available A fragmentação de hábitat causada pela ação humana vem sendo um dos grandes desafios para a biologia da conservação. A construção de estradas é um mecanismo de fragmentação de alto impacto, removendo a cobertura vegetal original, gerando efeito de borda e alterando a função e estrutura da paisagem, em contraste com a necessidade e o alto valor econômico imposto pela sociedade humana. Neste sentido, este estudo teve como objetivo avaliar o impacto da estrada na comunidade de fauna silvestre do Parque Altamiro de Moura Pacheco associada à presença da rodovia (BR-153/GO-060. Durante um ano de pesquisa, foram registrados 141 animais mortos por atropelamento. Os resultados do presente estudo, conduzido de Agosto/2003 a Julho/2004, foram comparados e analisados com estudo feito entre os anos de 1996 e 1997, tanto ao nível de carcaças encontradas quanto ao nível de ambiente. De acordo com os dados apresentados, reforça-se a necessidade de haver um melhor planejamento ao construir estradas, principalmente próximos a ambientes preservados ou unidades de conservação.The fragmentation of habitat caused by the action human being is comes one of the great challenges for the biology of the conservation. The construction of roads is a mechanism of the fragmentation of high impact, removing the original vegetablel covering, generating edge effect and modifying the function and structure of the landscape, in contrast with the necessity and the high economic value tax for the society human being. In this direction, this study it had as objective to evaluate the impact of the road in the community of wild fauna of the Park Altamiro de Moura Pacheco associated with the presence of the road (BR-153/GO-060. During one year of research, they had been registered 141 animals died for running over The results of the present study, lead of Agosto/2003 the Julho/2004, had been compared and analyzed with made study it enters the years of 1996 and 1997, as much the level of finded species how much the environment level. In accordance with the presented data, it is strengthened necessity to have one better planning when constructing roads, mainly next the preserved environments or units to conservation.

  19. Neutron Flux Depression in the UO{sub 2}-PuO{sub 2}(15 to 30%) Fuel Rods from IVO-FR2-Vg7-Irradiation Experiment; Depresion de flujo neutronico en las barras combustibles de UO2-PuO2(15 al 30%) del experimento de irradiacion IVO-FR2-Vg7

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, J.; Fernandez, J. L.

    1983-07-01

    The thermal-neutron flux depression within a fuel rod has a great influence in the radial temperature profile of the rod, especially for high enrichment fuel. For this reason, a study was made about the UO{sub 2}-PUO{sub 2} (15 to 30% PUO{sub 2}) fuel pins for the KfK-JEN joint irradiation program IVO, in the FR2 reactor. Different methods (diffusion, Bonalumi, successive generations) were compared and a new approach (parabolic approximation) was developed. (Author) 22 refs.

  20. A new sisorid catfish of the genus Gagata Bleeker from India

    NARCIS (Netherlands)

    Tilak, R.

    1970-01-01

    CONTENTS Introduction .................. 207 Description................... 207 Sexual dimorphism................. 211 Relationships .................. 211 A key to the species of the allied genera, Gagata Bleeker etc....... 213 Acknowledgements................. 215 References................... 215

  1. 12 CFR 337.3 - Limits on extensions of credit to executive officers, directors, and principal shareholders of...

    Science.gov (United States)

    2010-01-01

    ... principal shareholders of insured nonmember banks. (a) With the exception of 12 CFR 215.5(b), 215.5(c)(3... A of Federal Reserve Board Regulation O (12 CFR part 215, subpart A) to the same extent and to the... Regulation O (12 CFR 215.2(f)). (c)(1) No insured nonmember bank may extend credit in an aggregate...

  2. Investigation for determining the retention properties of rock over the ASSE II mine for dissolved radionuclides

    International Nuclear Information System (INIS)

    For a model consideration of groundwater movement and the associated transport of dissolved substance in deep groundwater, the retention properties of the ASSE II mine for radionuclides dissolved in water were determined. A series of properties of rocks were examined and described for this purpose. Apart from the chemical, mineralogical and petrophysical characteristics of the rocks, the retention was determined by 3 different methods and stated in the form of distribution coefficients for specific elements. A comparison of the results of vibration and diffusion experiments gave good agreement, while the results in through column experiments only reached the same order of magnitude after an expensive determination with the aid of a place concentration distribution. The distribution coefficients for the elements carbon, selenium, strontium, technetium, iodine, caesium, lead, radium, actinium, proto-actinium, uranium, neptunium, plutonium, americium and curium are listed and collected for model rock packages. (orig./HP)

  3. LASL experience in decontamination of the environment

    International Nuclear Information System (INIS)

    This discussion represents one part of a major effort in soil decontamination at the Los Alamos site. A contaminated industrial waste line in the Los Alamos townsite was removed, and a plutonium incineration facility, and a filter building contaminated with actinium-227 were dismantled. The former plutonium handling facility has been decontaminated, and canyons and an old firing site contaminated with strontium-90 have been surveyed

  4. Sorption studies of radioelements on geological materials

    OpenAIRE

    Berry, J. A; 油井 三和; 北村 暁

    2007-01-01

    Batch sorption experiments have been carried out to study the sorption of uranium, technetium, curium, neptunium, actinium, protactinium, polonium, americium and plutonium onto bentonite, granodiorite and tuff. Mathematical modelling using the HARPHRQ program and the HATCHES database was carried out to predict the speciation of uranium and technetium in the equilibrated seawater, and neptunium, americium and plutonium in the rock equilibrated water. Review of the literature for thermodynamic ...

  5. Study of the origin of elements of the uranium-235 family observed in excess in the vicinity of the experimental nuclear EL4 reactor under dismantling. Lessons got at this day and conclusions; Etude de l'origine des elements de la famille de l'uranium-235 observes en exces dans les environs du reacteur nucleaire experimental EL4 en cours de demantelement. Enseignements retires a ce jour et conclusion

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This study resumes the discovery of an excess of actinium 227 found around by EL4 nuclear reactor actually in dismantling. The search for the origin of this excess revealed a real inquiry of investigation during three years. Because a nuclear reactor existed in this area a particular attention will have concerned this region. The doubt became the line of conduct to find the answer to the human or natural origin of this excess. Finally and against any evidence, it appears that the origin of this phenomenon was natural, consequence of the particular local geology. The detail of the different investigations is given: search of a possible correlation with the composition of elevations constituent of lanes, search (and underlining) of new sites in the surroundings of the Rusquec pond and the Plouenez station, study of the atmospheric deposits under winds of the nuclear power plant and in the east direction, search of a possible relationship with the gaseous effluents of the nuclear power plant in the past, historical study of radioactive effluents releases in the fifty last years by the analysis of the sedimentary deposits in the Saint-Herbiot reservoir, search of a possible correlation between the excess of actinium 227 and the nuclear power plant activity; search of a possible correlation with a human activity without any relationship with the nuclear activities, search of a correlation with the underground waters, search of a correlation with the geological context, collect of information on the possible transfers in direction of the food chain, determination of the radiological composition of the underground waters ( not perturbed by human activity), search of the cause of an excess of actinium 227 in the old channel of liquid effluents release of the nuclear power plant. The results are given and discussed. And contrary to all expectations the origin of the excess of actinium 227 is completely natural. (N.C.)

  6. The discoveries of uranium 237 and symmetric fission — From the archival papers of Nishina and Kimura

    OpenAIRE

    IKEDA, Nagao

    2011-01-01

    Shortly before the Second World War time, Nishina reported on a series of prominent nuclear physical and radiochemical studies in collaboration with Kimura. They artificially produced 231Th, a member of the natural actinium series of nuclides, by bombarding thorium with fast neutrons. This resulted in the discovery of 237U, a new isotope of uranium, by bombarding uranium with fast neutrons, and confirmed that 237U disintegrates into element 93 with a mass number of 237. They also identified t...

  7. Hyperspectral surface materials map of quadrangles 3668 and 3768, Baghlan (221), Taluqan (222), Imam Sahib (215), and Rustaq (216) quadrangles, Afghanistan, showing iron-bearing minerals and other materials

    Science.gov (United States)

    King, Trude V.V.; Hoefen, Todd M.; Kokaly, Raymond F.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected iron-bearing minerals and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. This map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Goethite and jarosite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  8. Hyperspectral surface materials map of quadrangles 3668 and 3768, Baghlan (221), Taluqan (222), Imam Sahib (215), and Rustaq (216) quadrangles, Afghanistan, showing carbonates, phyllosilicates, sulfates, altered minerals, and other materials

    Science.gov (United States)

    Kokaly, Raymond F.; King, Trude V.V.; Hoefen, Todd M.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected carbonates, phyllosilicates, sulfates, altered minerals, and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. The map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Epidote or chlorite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  9. Report made on the behalf of the Commission of foreign affairs, defence and armed forces on the bill project authorizing the ratification of the international convention for the repression of acts of nuclear terrorism - Nr 215

    International Nuclear Information System (INIS)

    In a first part, this report recalls that the terrorist threat has already been addressed by texts which have been negotiated within the United Nations. It describes how the content of the convention has been elaborated while taking into account existing conventions related to nuclear issues. It outlines that this text is the fruit of the efforts made by a special committee created by the U N, and by the Lyons-Rome group within the G8. The second part comments how this convention is based on other conventions already ratified by France, notably the conventions of 1980 and 1997 on the physical protection of nuclear material. It outlines the important progress contained by this convention regarding incriminations of acts of nuclear terrorism and their crackdown

  10. From Soft Power to Economic Diplomacy? A Comparison of the Changing Rationales and Roles of the U. S. and Canadian Federal Governments in International Education. Research & Occasional Paper Series: CSHE.2.15

    Science.gov (United States)

    Trilokekar, Roopa Desai

    2015-01-01

    Through a historical and comparative analysis of international education policy development in Canada and the U.S., this paper will map the similarities and differences in the two countries. It will highlight the contributions and challenges of the government's involvement in international education (IE) in the two federal states and in…

  11. Classic 18.5- and 21.5-kDa myelin basic protein isoforms associate with cytoskeletal and SH3-domain proteins in the immortalized N19-oligodendroglial cell line stimulated by phorbol ester and IGF-1.

    Science.gov (United States)

    Smith, Graham S T; Homchaudhuri, Lopamudra; Boggs, Joan M; Harauz, George

    2012-06-01

    The 18.5-kDa classic myelin basic protein (MBP) is an intrinsically disordered protein arising from the Golli (Genes of Oligodendrocyte Lineage) gene complex and is responsible for compaction of the myelin sheath in the central nervous system. This MBP splice isoform also has a plethora of post-translational modifications including phosphorylation, deimination, methylation, and deamidation, that reduce its overall net charge and alter its protein and lipid associations within oligodendrocytes (OLGs). It was originally thought that MBP was simply a structural component of myelin; however, additional investigations have demonstrated that MBP is multi-functional, having numerous protein-protein interactions with Ca²⁺-calmodulin, actin, tubulin, and proteins with SH3-domains, and it can tether these proteins to a lipid membrane in vitro. Here, we have examined cytoskeletal interactions of classic 18.5-kDa MBP, in vivo, using early developmental N19-OLGs transfected with fluorescently-tagged MBP, actin, tubulin, and zonula occludens 1 (ZO-1). We show that MBP redistributes to distinct 'membrane-ruffled' regions of the plasma membrane where it co-localizes with actin and tubulin, and with the SH3-domain-containing proteins cortactin and ZO-1, when stimulated with PMA, a potent activator of the protein kinase C pathway. Moreover, using phospho-specific antibody staining, we show an increase in phosphorylated Thr98 MBP (human sequence numbering) in membrane-ruffled OLGs. Previously, Thr98 phosphorylation of MBP has been shown to affect its conformation, interactions with other proteins, and tethering of other proteins to the membrane in vitro. Here, MBP and actin were also co-localized in new focal adhesion contacts induced by IGF-1 stimulation in cells grown on laminin-2. This study supports a role for classic MBP isoforms in cytoskeletal and other protein-protein interactions during membrane and cytoskeletal remodeling in OLGs.

  12. Livelihoods and Learning: Education for All and the Marginalisation of Mobile Pastoralists. By Caroline Dyer. London: Routledge, 2014, 215 pp.; ISBN 978-0-415-58590-3 (Hardcover, 978-0-203-08390-1 (E-Book.

    Directory of Open Access Journals (Sweden)

    Alan Rogers

    2016-02-01

    Full Text Available In this book, Caroline Dyer, whose work with education in India has been known for many years through many articles, draws together her ethnographic experience of living with the Rabari of India, and reflects on what this means for Education for All goals.

  13. Study of the Quasielastic {sup 3}He(e,e{prime}p) Reaction at Q{sup 2}=1.5 (GeV/c){sup 2} up to Missing Momenta of 1 GeV/c

    Energy Technology Data Exchange (ETDEWEB)

    Marat Rvachev

    2003-09-01

    As a part of the E89044 experiment at Hall A of Jefferson Lab, we have studied the quasielastic {sup 3}He(e,e{prime}p) reaction in perpendicular coplanar kinematics, with energy and momentum transfer by the electron fixed at 837 MeV and 1500 MeV/c respectively, at three beam energies of 1255, 1954 and 4807 MeV. {sup 3}He(e,e{prime}p)D and {sup 3}He(e,e{prime}p)pn cross sections and distorted spectral functions were measured up to missing momenta of 1000 MeV/c, and, for the three-body breakup channel, up to missing energy of 30 MeV. The A{sub TL} asymmetry, R{sub T} and R{sub TL} response functions, and the combination R{sub L} + R{sub TT}V{sub TT}/V{sub L} of response functions were separated for the {sup 3}He(e,e{prime}p)D reaction channel up to missing momenta of 550 MeV/c. In the low missing momentum regime, measured {sup 3}He(e,e{prime}p)D cross sections agree with available calculations based on variational ground state wave functions, and disagree with calculations based on Faddeev-type ground state wave functions. For missing momenta from 100 to 740 MeV/c, strong final state interaction effects, in general consistent with Glauber-type and diagrammatic calculations, are observed. On a finer detail, meson exchange currents, isobaric currents and dynamical relativistic effects might be isolated with further theoretical work, in view of remaining disagreements between available calculations and the measurements. For missing momenta from 740 to 1000 MeV/c, measured {sup 3}He(e,e{prime}p)D cross sections are more than an order of magnitude greater than predicted by available theories. Further theoretical work is needed for understanding the nature of processes in this region.

  14. System for recovery of daughter isotopes from a source material

    Science.gov (United States)

    Tranter, Troy J [Idaho Falls, ID; Todd, Terry A [Aberdeen, ID; Lewis, Leroy C [Idaho Falls, ID; Henscheid, Joseph P [Idaho Falls, ID

    2009-08-04

    A method of separating isotopes from a mixture containing at least two isotopes in a solution is disclosed. A first isotope is precipitated and is collected from the solution. A daughter isotope is generated and collected from the first isotope. The invention includes a method of producing an actinium-225/bismuth-213 product from a material containing thorium-229 and thorium-232. A solution is formed containing nitric acid and the material containing thorium-229 and thorium-232, and iodate is added to form a thorium iodate precipitate. A supernatant is separated from the thorium iodate precipitate and a second volume of nitric acid is added to the thorium iodate precipitate. The thorium iodate precipitate is stored and a decay product comprising actinium-225 and bismuth-213 is generated in the second volume of nitric acid, which is then separated from the thorium iodate precipitate, filtered, and treated using at least one chromatographic procedure. A system for producing an actinium-225/bismuth-213 product is also disclosed.

  15. DISSOLVED CONCENTRATION LIMITS OF RADIOACTIVE ELEMENTS

    Energy Technology Data Exchange (ETDEWEB)

    P. Bernot

    2005-07-13

    The purpose of this study is to evaluate dissolved concentration limits (also referred to as solubility limits) of elements with radioactive isotopes under probable repository conditions, based on geochemical modeling calculations using geochemical modeling tools, thermodynamic databases, field measurements, and laboratory experiments. The scope of this activity is to predict dissolved concentrations or solubility limits for elements with radioactive isotopes (actinium, americium, carbon, cesium, iodine, lead, neptunium, plutonium, protactinium, radium, strontium, technetium, thorium, and uranium) relevant to calculated dose. Model outputs for uranium, plutonium, neptunium, thorium, americium, and protactinium are provided in the form of tabulated functions with pH and log fCO{sub 2} as independent variables, plus one or more uncertainty terms. The solubility limits for the remaining elements are either in the form of distributions or single values. Even though selection of an appropriate set of radionuclides documented in Radionuclide Screening (BSC 2002 [DIRS 160059]) includes actinium, transport of Ac is not modeled in the total system performance assessment for the license application (TSPA-LA) model because of its extremely short half-life. Actinium dose is calculated in the TSPA-LA by assuming secular equilibrium with {sup 231}Pa (Section 6.10); therefore, Ac is not analyzed in this report. The output data from this report are fundamental inputs for TSPA-LA used to determine the estimated release of these elements from waste packages and the engineered barrier system. Consistent modeling approaches and environmental conditions were used to develop solubility models for the actinides discussed in this report. These models cover broad ranges of environmental conditions so they are applicable to both waste packages and the invert. Uncertainties from thermodynamic data, water chemistry, temperature variation, and activity coefficients have been quantified or

  16. Detection of rare earth elements in Powder River Basin sub-bituminous coal ash using laser-induced breakdown spectroscopy (LIBS)

    Energy Technology Data Exchange (ETDEWEB)

    Tran, Phuoc [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State; Mcintyre, Dustin [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State

    2015-10-01

    We reported our preliminary results on the use of laser-induced breakdown spectroscopy to analyze the rare earth elements contained in ash samples from Powder River Basin sub-bituminous coal (PRB-coal). We have identified many elements in the lanthanide series (cerium, europium, holmium, lanthanum, lutetium, praseodymium, promethium, samarium, terbium, ytterbium) and some elements in the actinide series (actinium, thorium, uranium, plutonium, berkelium, californium) in the ash samples. In addition, various metals were also seen to present in the ash samples

  17. Low background counting of 222Rn, 220Rn and 219Rn with electrostatic counters

    Science.gov (United States)

    Mong, Brian; EXO-200 Collaboration; nEXO Collaboration

    2014-09-01

    The radon counting technique based on electrostatic precipitation of progenies in gas followed by alpha spectroscopy has been applied to support the material selection programs of low background, neutrino and dark matter experiments with emphasis on EXO. An array of 8 counters operated by Laurentian University at SNOLAB and the Waste Isolation Pilot Plant have reached the sensitivity of 10 atoms/day in the uranium, thorium and actinium chains. Hardware improvements are underway to further increase the capacity and sensitivity in support of nEXO. The radon counting technique based on electrostatic precipitation of progenies in gas followed by alpha spectroscopy has been applied to support the material selection programs of low background, neutrino and dark matter experiments with emphasis on EXO. An array of 8 counters operated by Laurentian University at SNOLAB and the Waste Isolation Pilot Plant have reached the sensitivity of 10 atoms/day in the uranium, thorium and actinium chains. Hardware improvements are underway to further increase the capacity and sensitivity in support of nEXO. Supported by NSERC Project Grants ``Search for Double Beta Decay with EXO.''

  18. Purification of cerium, neodymium and gadolinium for low background experiments

    Science.gov (United States)

    Boiko, R. S.; Barabash, A. S.; Belli, P.; Bernabei, R.; Cappella, F.; Cerulli, R.; Danevich, F. A.; Incicchitti, A.; Laubenstein, M.; Mokina, V. M.; Nisi, S.; Poda, D. V.; Polischuk, O. G.; Tretyak, V. I.

    2014-01-01

    Cerium, neodymium and gadolinium contain double beta active isotopes. The most interesting are 150Nd and 160Gd (promising for 0ν2β search), 136Ce (2β+ candidate with one of the highest Q2β). The main problem of compounds containing lanthanide elements is their high radioactive contamination by uranium, radium, actinium and thorium. The new generation 2β experiments require development of methods for a deep purification of lanthanides from the radioactive elements. A combination of physical and chemical methods was applied to purify cerium, neodymium and gadolinium. Liquid-liquid extraction technique was used to remove traces of Th and U from neodymium, gadolinium and for purification of cerium from Th, U, Ra and K. Co-precipitation and recrystallization methods were utilized for further reduction of the impurities. The radioactive contamination of the samples before and after the purification was tested by using ultra-low-background HPGe gamma spectrometry. As a result of the purification procedure the radioactive contamination of gadolinium oxide (a similar purification efficiency was reached also with cerium and neodymium oxides) was decreased from 0.12 Bq/kg to 0.007 Bq/kg in 228Th, from 0.04 Bq/kg to <0.006 Bq/kg in 226Ra, and from 0.9 Bq/kg to 0.04 Bq/kg in 40K. The purification methods are much less efficient for chemically very similar radioactive elements like actinium, lanthanum and lutetium.

  19. Mixed valency of the RE and Ac ions in solid states

    International Nuclear Information System (INIS)

    Full text: This review is devoted to consideration of the methods and results of the theoretical and experimental investigations of the electronic state of the rare earths and actinium ions in the separate compound. The methods of the high resolution X ray spectroscopy were used for our consideration. Energy of the X ray lines of the rare earths and actinium ions in free state and solid states was study carefully. Valency shifts of X Ray lines under change of electronic state of nf - ions in different oxide, fluoride and chloride compounds were determined for K -, L- and M - lines for these ions with accuracy near 10 -100 MeV. We studied the electronic state of nf- ions which is changed from '+2' to '+4' and determined the concentration of ions in different electronic state. Relative error for theoretical energy of the most lines for HFP - approach in comparison with experiment is less than 0.5%, and deviation for concentration is less than 5%

  20. The Technological Enhancement of Normally Occurring Radioactive Materials in Red Mud due to the Production of Alumina

    Directory of Open Access Journals (Sweden)

    Maurice O. Miller

    2016-01-01

    Full Text Available This study investigates the level of technological enhancement of normally occurring radioactive materials (TENORM in the red mud waste due to the production of alumina in Jamaica. Technological enhancements factors (TEF were determined for the uranium, thorium, actinium series, their progenies, and the nonseries potassium-40 using gamma spectrometry. The study concluded that bauxite production technologically enhances the uranium progenies Th-234, Pb-214, Bi-214, and Pa-234 and the thorium-232 progenies Ac-228, Pb-212, and Bi-212 in red mud. The actinium series was technologically enhanced, but K-40 and the thorium daughter, Tl-208, were reduced. The spectrometric comparison of Tl-208 (at 510 keV was unexpected since its other photopeaks at 583 keV, 934 keV, and 968 keV were markedly different. An explanation for this anomaly is discussed. An explanation regarding the process of accumulation and fractionation of organically derived phosphate deposits and potassium-feldspar is offered to explain the spectrometric differences between the alumina product and its waste material, red mud.

  1. A study of uranium and thorium migration at the Koongarra uranium deposit with application to actinide transport from nuclear waste repositories

    International Nuclear Information System (INIS)

    One way to gain confidence in modelling possible radionuclide releases is to study natural systems which are similar to components of the multibarrier waste repository. Several such analogues are currently under study and these provide useful data about radionuclide behaviour in the natural environment. One such system is the Koongarra uranium deposit in the Northern Territory. In this dissertation, the migration of actinides, primarily uranium and thorium, has been studied as an analogue for the behaviour of transuranics in the far-field of a waste repository. The major findings of this study are: 1. the main process retarding uranium migration in the dispersion fan at Koongarra is sorption, which suppresses dissolved uranium concentrations well below solubility limits, with ferrihydrite being a major sorbing phase; 2. thorium is extremely immobile, with very low dissolved concentrations and corresponding high distribution ratios for 230Th. Overall, it is estimated that colloids are relatively unimportant in Koongarra groundwater. Uranium migrates mostly as dissolved species, whereas thorium and actinium are mostly adsorbed to larger, relatively immobile particles and the stationary phase. However, of the small amount of 230Th that passes through a 1μm filter, a significant proportion is associated with colloidal particles. Actinium appears to be slightly more mobile than thorium and is associated with colloids to a greater extent, although generally present in low concentrations. These results support the possibility of colloidal transport of trivalent and tetravalent actinides in the vicinity of a nuclear waste repository. 112 refs., 23 tabs., 32 figs

  2. Purification of cerium, neodymium and gadolinium for low background experiments

    Directory of Open Access Journals (Sweden)

    Boiko R.S.

    2014-01-01

    Full Text Available Cerium, neodymium and gadolinium contain double beta active isotopes. The most interesting are 150Nd and 160Gd (promising for 0ν2β search, 136Ce (2β+ candidate with one of the highest Q2β. The main problem of compounds containing lanthanide elements is their high radioactive contamination by uranium, radium, actinium and thorium. The new generation 2β experiments require development of methods for a deep purification of lanthanides from the radioactive elements. A combination of physical and chemical methods was applied to purify cerium, neodymium and gadolinium. Liquid-liquid extraction technique was used to remove traces of Th and U from neodymium, gadolinium and for purification of cerium from Th, U, Ra and K. Co-precipitation and recrystallization methods were utilized for further reduction of the impurities. The radioactive contamination of the samples before and after the purification was tested by using ultra-low-background HPGe gamma spectrometry. As a result of the purification procedure the radioactive contamination of gadolinium oxide (a similar purification efficiency was reached also with cerium and neodymium oxides was decreased from 0.12 Bq/kg to 0.007 Bq/kg in 228Th, from 0.04 Bq/kg to <0.006 Bq/kg in 226Ra, and from 0.9 Bq/kg to 0.04 Bq/kg in 40K. The purification methods are much less efficient for chemically very similar radioactive elements like actinium, lanthanum and lutetium.

  3. « Communities in Place and Communities in Space. Globalization and Féminism in Iran », in : Parvin Alizadeh, ed., The Economy of Iran: Dilemmas of an Islamic State. London - New York, Tauris, 2002, pp. 215-224.

    OpenAIRE

    Ladier-Fouladi, Marie

    2006-01-01

    Dans cet article, l’A. propose de montrer en premier lieu qu’en dépit de la politique discriminatoire de la République islamique, le statut de la femme s’est amélioré depuis 1979. En second lieu, il tente de déterminer les facteurs à l’origine de cette évolution. Pour l’A., la diffusion de l’école à une échelle géographique de plus en plus étendue et notamment l’accroissement du niveau d’instruction scolaire des femmes d’une part, et la globalisation, d’autre part, constituent les deux facteu...

  4. « Sexuality, Rights and Islam: Competing Gender Discourses in Postrevolutionary Iran », in : Lois Beck & Guity Nashat, dirs., Women in Iran. From 1800 to the Islamic Republic. Urbana & Chicago, University of Illinois Press, 2004, pp. 204-215.

    OpenAIRE

    Kian-Thiébaut, Azadeh

    2007-01-01

    L’A. divise les discours religieux existants en Iran sur les femmes en traditionaliste, néo-traditionaliste et moderniste. Les questions de l’égalité entre les sexes et les droits des femmes sont absentes du premier discours centré sur la différence entre les sexes (représenté notamment par l’Ayatollah Meshkini, président de l’Assemblée des Experts). Le deuxième (fondé notamment sur les enseignements de l’Ayatollah Mottahari) est sensible aux débats sur le genre et aux critiques formulées par...

  5. Relationship between dietary patterns and metabolic syndrome among urban populations in Ningxia, China . Journal of Zhejiang University (Agric . & Life Sci .), 2013,39(2):215-221%宁夏城市居民营养素摄入量与代谢综合征相关危险因素调查及分析

    Institute of Scientific and Technical Information of China (English)

    李宏宇; 张娜; 李琳; 陶秀娟; 杨建军; 张毓洪; 赵燚

    2013-01-01

    Summary It is well documented that the role of dietary composition in relation to the development of metabolic syndrome ( MS) remains inconclusive . Several studies on the relationship between MS and energy intake have suggested that the macronutrient composition of the diet ( proteins , fats , carbohydrates) may play an important contributing role to MS in adults . So , this study aims to investigate the possible relationship between MS and energy intake , percentage energy intake from macronutrients among urban populations in Ningxia , China . According to different economic levels from 2011 to 2012 , 2 465 subjects ( age:18‐75 years) were selected using stratified cluster random sampling in Yinchuan , Wuzhong , Zhongwei , Guyuan , and Shizuishan in Ningxia , China . Dietary intake was assessed using 24‐h food records and a validated food frequency questionnaire . Body mass , height , waist circumference , blood pressure , triglyceride ( TG ) , high‐density lipoprotein cholesterol ( HDL‐C ) , and blood glucose were measured . Logistic regression models were adjusted for age , smoking , drinking , and physical activity factors . The results showed that there were 647 MS cases in all subjects . According to the diagnosis criteria of MS , the incidence of MS was 26 .2% ( men:281 cases (25% ) ;women:366 cases ( 27 .3% ) ) , no significant difference in sex composition ( P>0 .05) . As a way of lifestyle , there was no significant difference in smoking , drinking , exercise between MS group and control group ( P>0 .05) ;significant difference was found in tertian dietary intake of protein and fat energy percentage between MS group and control group ( P0.05);MS组与对照组间吸烟、饮酒、锻炼等生活方式构成的差异无统计学意义( P>0.05);MS组与对照组间日膳食摄入蛋白质及脂肪能量百分比差异有统计学意义( P<0.05);MS组与对照组间的体质指数、腰围、收缩压、舒张压、空腹血糖、胆固醇、甘油三酯、高密度脂蛋白差异有统计学意义( P<0.05). Logistic多因素分析表明,蛋白质、脂肪及碳水化合物的能量占总摄入能量的百分比过多是城市居民代谢综合征的重要危险因素,其中高蛋白质组:相对危险程度比值比( odds ratio , OR)=1.417;高脂肪组:OR=1.647;高碳水化合物组:OR=1.810;因此,应采取膳食营养干预措施来有效预防MS .

  6. Dicty_cDB: Contig-U08414-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available gtcgttgnattatctaca 70 Query: 71 acttttctttcnccnactgttgaattaaccgtncaactcaataatgatgattcttcaact 130 |||||||||||...||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 71 acttttctttcnccnactgtt...|||||||||||||||||| Sbjct: 275 aaattaggaagtgcacctttgataaatcaattctaaanncngtcgttgnattatctacaa 216 Query: 72 cttttctttcnccna...|||||||||||||| Sbjct: 215 cttttctttcnccnactgttgaattaaccgtncaactcaataatgatgattcttcaactc 156 Query: 132 atacca

  7. Gene : CBRC-MDOM-04-0426 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available 50 /gi=89886357 /ug=Mdm.215 /len=1443 0.014 18% MPTTTSTTTSTITSTTTTTITSTFTITSTFTITSTTTSTTTTTITITSTTTTITS...TSTTTITSTSTITSTTTTTITSTSTITSTTTITSTTSTTITSTTTTITSTTTTTSTTTTTTITSTSTITSTTTTTITSTSTTTTTITSTTTTTITSTSITSTTTTTTITS...TSTITSTTTTTTTTSTTITSTTTITSTTTTTITSTTSSTSTTTTTTSTTITSTTTTSTTTITSTTITSTITTTSTTITSTTTITSTTTTTITSTTTITSTTTTTITS...TTSSTSTTTTTSTTITSTTTTSTTTITSTTTTTITSTTSSTSTTTTTSTTITSTPTTSTTTTSTTITSTTTTSTTITS...TTTITSTTTTTITSASTTTTTTITSTSTTITSTTTTTITSTSTITSTTTTTITSTSTITSITTSTITSTSTITSTTTTTSTTITSTTTITSTTTTTTTSTTTTSTTTSATITS

  8. Protein (Viridiplantae): 357448019 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available :12299 3880:12299 14 kDa proline-rich protein DC2.15 Medicago truncatula MGLKGVGSIFILFLHLIFVSTLVSAKSAPRRPPPPPQVTCSSTPPPVVAPSTPPSTPP...ITVAPSTSPVAVDPTTLPPSTPPSTPPTSTPPNVVAPSTPPPSTPPFTTPPTVAPSTPPLVHAPSTPPPSTPPSTPP...TAIAPSTPQVVIDPTTLPPSTTHSTPPSVDAPSSPPSTPPTTVAPSTPPSLVDPTTPPPSTPPSTPPPVHDPSTPPSTPPIAVAPSTPPSVVNPTIPPPSTPPTTPPPNPRTPP...TNPRTPPTNPRTPPTTPPPNPRTPPTTPPTNPRTPPKNPMTPPTIPPPNPRTPPTTLPPNPRTPPTTPPANPRTPPTRPPTNPRTPPTTSPPNPRTPPTNPMTPP...TTPPANPRTPPTTPPTNPRTPPTTPPTNPRTPPTTPPPNPRTPPTNPMKPPTTPPPNPMTPPSTPPPNPRTPPTTPSPNPRTPP

  9. Protein (Viridiplantae): 356514232 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available 3847:61 PREDICTED: 14 kDa proline-rich protein DC2.15-like Glycine max MGSKTRSSLAIFLTVNILFFALVSACGTCPSPTPRHKHKPVKPSPSGGSGGSGGSGGSGG...SGGSGGSGGSGGSGGSGGSGGSGGSGASCPRDALKLGVCANVLNGLLNVTLGQPPVTPCCSLLNGLVDLEAAVCLCTALKANILGINLNLPISLSLLLNVCSRNAPRDFQCA ...

  10. Barbara Fuchs & Emily Weissbourd (eds., Representing Imperial Rivalry in the Early Modern Mediterranean (Toronto: Toronto University Press, 2015

    Directory of Open Access Journals (Sweden)

    Viviana Tagliaferri

    2016-03-01

    Full Text Available Book review of: Barbara Fuchs, Emily Weissbourd (eds., Representing Imperial Rivalry in the Early Modern Mediterranean, (Toronto: Toronto University Press, 215, pp. 296, ISBN 9781442619272.

  11. EST Table: FS904669 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS904669 E_FL_fufe_02L06_F_0 10/09/28 39 %/215 aa ref|XP_967337.1| PREDICTED: similar to Serge... %/215 aa gi|91083383|ref|XP_967337.1| PREDICTED: similar to Sergef protein [Tribolium castaneum] FS910504 fufe ...

  12. EST Table: CK516660 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK516660 rswjb0_004566.y1 11/12/09 n.h 10/09/29 71 %/215 aa ref|XP_968436.2| PREDICTED: similar to best... %/176 aa gnl|Amel|GB16861-PA 10/09/10 71 %/215 aa gi|189235903|ref|XP_968436.2| PREDICTED: similar to bes

  13. Vegetation abundance in lowland flood plain lakes determined by surface area, age and connectivity

    NARCIS (Netherlands)

    Geest, van G.J.; Roozen, F.C.J.M.; Coops, H.; Roijackers, R.M.M.; Buijse, A.D.; Peeters, E.T.H.M.; Scheffer, M.

    2003-01-01

    The authors analyzed the vegetation structure of 215 lakes in the flood plain of the river Lower Rhine in relation to environmental variables related to hydrological connectivity, lake morphometry, lake age and land use on adjacent land1. We analysed the vegetation structure of 215 lakes in the floo

  14. Drug: D08624 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D08624 Drug Tramazoline (INN); Muconasal (TN) C13H17N3 215.1422 215.2942 D08624.gif...970(146+147+148) Salivary secretion Anatomical Therapeutic Chemical (ATC) classification [BR:br08303] R RESPIRATORY SYSTEM R01 NASA...L PREPARATIONS R01A DECONGESTANTS AND OTHER NASAL PREPARATIONS FOR TOPICAL USE R01AA

  15. 77 FR 59244 - Environmental Impact Statement: Riverside County, CA; Notice of Intent

    Science.gov (United States)

    2012-09-26

    ... Riverside County; and (3) to improve roadway alignment and intersection design to enhance safety along... alignment changes between I- 215 and Temescal Canyon Road; (2) widening existing Cajalco Road between I-215... to private organizations and citizens who have previously expressed or are known to have interest...

  16. EST Table: FS754028 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS754028 E_ET_caL-_14O17_R_0 10/09/28 63 %/215 aa ref|XP_002093380.1| GE20793 [Dros...ophila yakuba] gb|EDW93092.1| GE20793 [Drosophila yakuba] 10/09/08 63 %/215 aa FBpp0265803|DyakGE20793-PA 10

  17. EST Table: FS868205 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS868205 E_FL_fner_36C20_R_0 10/09/28 65 %/215 aa ref|XP_002093380.1| GE20793 [Dros...ophila yakuba] gb|EDW93092.1| GE20793 [Drosophila yakuba] 10/09/11 65 %/215 aa FBpp0265803|DyakGE20793-PA 10

  18. EST Table: FY762792 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY762792 E_FL_famL_45L23_R_0 11/11/04 65 %/215 aa ref|XP_002093380.1| GE20793 [Dros...ophila yakuba] gb|EDW93092.1| GE20793 [Drosophila yakuba] 11/11/04 65 %/215 aa FBpp0265803|DyakGE20793-PA 11

  19. Drug: D06125 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D06125 Drug Tiamenidine (USAN/INN); Symcor base TTS (TN) C8H10ClN3S 215.0284 215.7031 D06125.gif Antihyperte...nsive CAS: 31428-61-2 PubChem: 47207783 LigandBox: D06125 NIKKAJI: J20.515J ATOM 13

  20. Drug: D04905 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available HCl 215.1441 215.7628 D04905.gif Treatment of Alzheimer's disease [DS:H00056] Therapeutic category: 1190 ATC...3+2904+2905+2906) Glutamatergic synapse hsa05010(2902+2903+2904+2905+2906) Alzheimer's disease Transporter: ...SLC22A2 [HSA:6582] map07056 Agents for Alzheimer-type dementia map07235 N-Metyl-D

  1. 78 FR 25093 - Multistate Conservation Grant Program; Priority List and Approval for Conservation Projects

    Science.gov (United States)

    2013-04-29

    ... Benefits to the States. 7......... Implementation of Strategic Wildlife 215,600 0 215,600 Tools to Evaluate... fisheries and wildlife management and research projects, boating access development, hunter safety and... enabling legislation. To be eligible for funding, a project must benefit fish and/or wildlife...

  2. Radionuclide interactions with marine sediments

    International Nuclear Information System (INIS)

    A critical review of the literature on the subject of the interactions of radionuclides with marine sediments has been carried out. On the basis of the information available, an attempt has been made to give ranges and 'best estimates' for the distribution ratios between seawater and sediments. These estimates have been based on an understanding of the sediment seawater system and the porewater chemistry and mineralogy. Field measurements, laboratory measurements and estimates based on stable-element geochemical data are all taken into account. Laboratory measurements include distribution-ratio and diffusion-coefficient determinations. The elements reviewed are carbon, chlorine, calcium, nickel, selenium, strontium, zirconium, niobium, technetium, tin, iodine, caesium, lead, radium, actinium, thorium, protactinium, uranium, neptunium, plutonium, americium and curium. (author)

  3. Process for radioisotope recovery and system for implementing same

    Science.gov (United States)

    Meikrantz, David H.; Todd, Terry A.; Tranter, Troy J.; Horwitz, E. Philip

    2007-01-02

    A method of recovering daughter isotopes from a radioisotope mixture. The method comprises providing a radioisotope mixture solution comprising at least one parent isotope. The at least one parent isotope is extracted into an organic phase, which comprises an extractant and a solvent. The organic phase is substantially continuously contacted with an aqueous phase to extract at least one daughter isotope into the aqueous phase. The aqueous phase is separated from the organic phase, such as by using an annular centrifugal contactor. The at least one daughter isotope is purified from the aqueous phase, such as by ion exchange chromatography or extraction chromatography. The at least one daughter isotope may include actinium-225, radium-225, bismuth-213, or mixtures thereof. A liquid-liquid extraction system for recovering at least one daughter isotope from a source material is also disclosed.

  4. Proposed training program for construction personnel involved in remedial action work at sites contaminated by naturally occurring radionuclides

    International Nuclear Information System (INIS)

    Many sites used during the early days of the US atomic energy program are contaminated with radionuclides of the primordial decay chains (uranium, thorium, and actinium series). This contamination consists of residues resulting from refining and processing uranium and thorium. Preparation of these sites for release to unrestricted private use will involve the assistance of construction workers, many of whom have limited knowledge of the hazards associated with radioactive materials. Therefore, there is a need to educate these workers in the fundamentals of radioactive material handling to minimize exposures and possible spread of contamination. This training should disseminate relevant information at an appropriate educational level and should instill a cautious, common-sense attitude toward the handling of radioactive materials. The training should emphasize basic information concerning environmental radiation within a context of relative risk. A multi-media format, including colorful visual aids, demonstration, and discussion, should be used to maximize motivation and retention. A detailed, proposed training program design is presented

  5. JAEA thermodynamic database for performance assessment of geological disposal of high-level and TRU wastes. Refinement of thermodynamic data for trivalent actinoids and samarium

    International Nuclear Information System (INIS)

    Within the scope of the JAEA thermodynamic database project for performance assessment of geological disposal of high-level radioactive and TRU wastes, the refinement of the thermodynamic data for the inorganic compounds and complexes of trivalent actinoids (actinium(III), plutonium(III), americium(III) and curium(III)) and samarium(III) was carried out. Refinement of thermodynamic data for these elements was based on the thermodynamic database for americium published by the Nuclear Energy Agency in the Organisation for Economic Co-operation and Development (OECD/NEA). Based on the similarity of chemical properties among trivalent actinoids and samarium, complementary thermodynamic data for their species expected under the geological disposal conditions were selected to complete the thermodynamic data set for the performance assessment of geological disposal of radioactive wastes. (author)

  6. Theoretical Study on Properties of New Isotope 265Bh

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The properties of nuclei belonging to the newly observed α-decay chain starting from 265Bh have been studied. The axially deformed relativistic mean-field calculation with the force NL-Z2 has been performed in the blockedBCS approximation. Some ground state properties such as binding energies, deformations, spins, and parities, as well as Q-values of the α-decay for this decay chain have been calculated and compared with known experimental data. Good agreement is found. The single-particle spectrum of the nucleus 265 Bh is studied and some new magic numbers are found,while the magnitudes of the shell gaps in superheavy nuclei are much smaller than those of nuclei before the actinium region, and the Fermi surfaces are close to the continuum. Thus the superheavy nuclei are usually not stable. The The methods which give good agreement with the data are selected.

  7. Efficient autophosphorylation and phosphorylation of the beta-subunit by casein kinase-2 require the integrity of an acidic cluster 50 residues downstream from the phosphoacceptor site

    DEFF Research Database (Denmark)

    Boldyreff, B; Meggio, F; Pinna, L A;

    1994-01-01

    -64 are also involved in the process of autophosphorylation, possibly by means of a loop formation. The results obtained with the COOH-terminal-deleted mutants support the view that reconstitution of a functional holoenzyme must occur to allow efficient autophosphorylation. Polylysine prevents...... mutants reconstituting a tetrameric holoenzyme. Only with the three largest COOH-terminal deletion mutants beta delta 150-215, beta delta 171-215, and beta delta 181-215 is no significant alpha-subunit autophosphorylation observed. The phosphorylation of the beta-subunit mutants added in large molar...... excess to CK-2 holoenzyme (either native or recombinant) is also severely impaired by Ala for Glu/Asp substitutions at position 5,6 and in the 55-64 region and by the deletion of the COOH-terminal segments 150-215 and 171-215. Such a phosphorylation is inhibited by polylysine, with the exception...

  8. Origin of elements of the Uranium-235 family observed in the Ellez river near the EL-4 experimental nuclear reactor in dismantling (Monts d'Arree- Finistere department); Origine des elements de la famille de l'uranium-235 observes dans la riviere Ellez a proximite du reacteur nucleaire experimental EL4 en cours de demantelement (Mont d'Arree - departement du Finistere). Resultats et premiers constats annee 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    In a previous study which concerned the catchment basin of the harbour of Brest, the A.C.R.O. put in evidence a marking by artificial radioelements around the power plant of Brennilis which can be imputed without ambiguities to the nuclear installation. It also put in evidence abnormalities concerning the natural radioactivity which justifies this new study. In the area of the Monts d'Arree, actinium 227 ({sup 227}Ac), non born by its ascendents which are {sup 235}U and {sup 231}Pa is observed. This phenomenon is characterized by mass activities superior to these ones of {sup 235}U and able to reach these ones of {sup 238}U. Its presence corresponds with the drainage of the Ellez river since the former channel of radioactive effluents releases from the nuclear power plant EL-4 up to the reservoir Saint-Herblot situated 6 km downstream. The strongest values of radioactivity are registered near the disused power plant, at this place a relationship exists between the level of actinium 227 and this one of the artificial radioactivity as it exists a relationship with the decay products of radon exhaled from the subsoil ({sup 210}Pb). But its presence is not limited to a part of the Ellez river, it is equally observed in terrestrial medium, in places in priori not influenced by the direct liquid effluents of the power plant. This place is situated at more than 4 km and without any connection with the Ellez waters. At this stage of the study, it is not possible to answer with certainty the question of the origin of this phenomenon. A new reorientation is considered indispensable to clarify definitively the origin of this unknown phenomenon in the scientific publications and the environmental monitoring. (N.C.)

  9. origin of elements of the Uranium-235 family observed in the Ellez river near the EL-4 experimental nuclear reactor in dismantling (Monts d'Arree- Finistere department)

    International Nuclear Information System (INIS)

    In a previous study which concerned the catchment basin of the harbour of Brest, the A.C.R.O. put in evidence a marking by artificial radioelements around the power plant of Brennilis which can be imputed without ambiguities to the nuclear installation. It also put in evidence abnormalities concerning the natural radioactivity which justifies this new study. In the area of the Monts d'Arree, actinium 227 (227Ac), non born by its ascendents which are 235U and 231Pa is observed. This phenomenon is characterized by mass activities superior to these ones of 235U and able to reach these ones of 238U. Its presence corresponds with the drainage of the Ellez river since the former channel of radioactive effluents releases from the nuclear power plant EL-4 up to the reservoir Saint-Herblot situated 6 km downstream. The strongest values of radioactivity are registered near the disused power plant, at this place a relationship exists between the level of actinium 227 and this one of the artificial radioactivity as it exists a relationship with the decay products of radon exhaled from the subsoil (210Pb). But its presence is not limited to a part of the Ellez river, it is equally observed in terrestrial medium, in places in priori not influenced by the direct liquid effluents of the power plant. This place is situated at more than 4 km and without any connection with the Ellez waters. At this stage of the study, it is not possible to answer with certainty the question of the origin of this phenomenon. A new reorientation is considered indispensable to clarify definitively the origin of this unknown phenomenon in the scientific publications and the environmental monitoring. (N.C.)

  10. 两种稀土产品中放射性核素分析研究%ANALYSIS OF RADIONUCLIDES IN TWO RARE EARTH PRODUCTS

    Institute of Scientific and Technical Information of China (English)

    周程; 孙恋君

    2009-01-01

    利用高纯锗γ谱仪对两个稀土产品氯化镧和氯化钇的样品进行能谱分析,研究经湿法冶炼工艺后的稀土产品中放射性核素的活度变化.结果表明,样品中存在天然放射性钍系和锕-铀系的核素,以及天然放射性核素~(138)La和~(176)Lu,但两个样品中所含的放射性核素不相同.氯化镧产品中锕-铀系和钍系核素放射性未达平衡,其主要子体~(224)Ra和~(227)Th的含量随时间发生明显变化,约60天达到平衡;而氯化钇产品没有这种现象.%This paper describes a study on raidoactivity change of rare-earth products after hydrometallurgy technology,using HPGe gamma spectrometer to analyze two rare earth products (LaCl_3 and YCl_3).The results show that in the two samples exist natural radionuclides of thorium series and actinium-uranium series as well as other natural radionuclides 138 La and 176 Lu.In the sample of lanthanum chloride the radioactivity of actinium-uranium series and thorium series has not reached balance,the contents of their main progenies ~(226)Ra and ~(227)Th obviously changes with the time,reaching balance 60 days later,while YCl_3 keeps stability.

  11. Arginine synthesis from enteral glutamine in healthy adults in the fed state.

    Science.gov (United States)

    Tomlinson, Chris; Rafii, Mahroukh; Ball, Ronald O; Pencharz, Paul

    2011-08-01

    Recent studies have documented transfer of labeled nitrogen from [2-(15)N]glutamine to citrulline and arginine in fasting human adults. Conversely, in neonates and piglets we have shown no synthesis of arginine from [2-(15)N]glutamate, and others have shown in mice that glutamine is a nitrogen, but not a carbon donor, for arginine synthesis. Therefore, we performed a multitracer study to determine whether glutamine is a nitrogen and/or carbon donor for arginine in healthy adult men. Two glutamine tracers, 2-(15)N and 1-(13)C, were given enterally to five healthy men fed a standardized milkshake diet. There was no difference in plasma enrichments between the two glutamine tracers. 1-(13)C isotopomers of citrulline and arginine were synthesized from [1-(13)C]glutamine. Three isotopomers each of citrulline and arginine were synthesized from the [2-(15)N]glutamine tracer: 2-(15)N, 5-(15)N, and 2,5-(15)N(2). Significantly greater enrichment was found of both [5-(15)N]arginine (0.75%) and citrulline (3.98%) compared with [2-(15)N]arginine (0.44%) and [2-(15)N]citrulline (2.62%), indicating the amino NH(2) from glutamine is mostly transferred to arginine and citrulline by transamination. Similarly, the enrichment of the 1-(13)C isotopomers was significantly less than the 2-(15)N isotopomers, suggesting rapid formation of α-ketoglutarate and recycling of the nitrogen label. Our results show that the carbon for 50% of newly synthesized arginine comes from dietary glutamine but that glutamine acts primarily as a nitrogen donor for arginine synthesis. Hence, studies using [2-(15)N]glutamine will overestimate arginine synthesis rates.

  12. Epidemiological Investigation of Lifestyle Associated Modifiable Risk Factors among Medical Students

    Directory of Open Access Journals (Sweden)

    Anurag Srivastava

    2013-06-01

    Conclusion: The study shows a high prevalence of NCD related risk factors in medical students. Primary prevention may be one way to lower the risk burden of NCDs. [Natl J Med Res 2013; 3(3.000: 210-215

  13. 76 FR 65719 - Wyco Power and Water, Inc.; Notice of Preliminary Permit Application Accepted for Filing and...

    Science.gov (United States)

    2011-10-24

    ... Power and Water, Inc., 1436 West Oak, Fort Collins, CO 80521, phone (970) 215-2603. FERC Contact: Jim... reservoirs: (1) The proposed Cactus Hill Reservoir (185,000 acre- feet in capacity), near Fort...

  14. Drug: D09967 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ide bridge: 23-89; 135-195; H230-L215, Dimer) Peptide Uveitis, rheumatoid arthritis, psoriasis ATC code: L04...teraction hsa05323(3605) Rheumatoid arthritis map07050 Antirheumatics - DMARDs and biological agents Anatomi

  15. Data of evolutionary structure change: 1BIJA-1YGDD [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1BIJA-1YGDD 1BIJ 1YGD A D -VLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPT...SKYR MHLTPEEKSAVTALWGKVN--VDEVGGEALGRLLVVYPETQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGT---F... 215 VAL CA 288 ASP CA 212 1YGD D 1YGDD AFSDGLAHLDNLK 1 1YGD

  16. Genetic variation for growth rate, feed conversion efficiency, and disease resistance exists within a farmed population of rainbow trout

    DEFF Research Database (Denmark)

    Henryon, Mark; Jokumsen, Alfred; Berg, Peer;

    2002-01-01

    The objective of this study was to test that additive genetic (co)variation for survival, growth rate, feed conversion efficiency, and resistance to viral haemorrhagic septicaemia (VHS) exists within a farmed population of rainbow trout. Thirty sires and 30 dams were mated by a partly factorial...... on days 52, 76, 96, 123, 157, 185, and 215, and body length on days 52 and 215); feed conversion efficiency between days 52-215, 52-76, 77-96, 97-123, 124-157, 158-185, and 186-215, and VHS resistance. REML estimates of additive genetic variation for the body weights, body lengths, and feed conversion...... efficiencies were obtained by fitting univariate linear (reduced) animal models. Additive genetic variation for VHS resistance was estimated by fitting a Weibull, sire-dam frailty model to time until death of fish challenged with VHS. Genetic correlations were estimated among the body weights, body length...

  17. Nearshore marine fish assemblages in southern California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Fish benthic trawls were completed by the Southern California Coastal Water Research Project (SCCWRP). Data from 425 fisheries independent trawls ranging from 2-215...

  18. 75 FR 69397 - Minnkota Power Cooperative, Inc.: Notice of Availability of an Environmental Assessment

    Science.gov (United States)

    2010-11-12

    ... Independence Ave., SW., Room 2244-S, Stop 1571, Washington, DC 20250-1571, fax: (202) 690-0649, or e-mail... Auditor......... 115 West Main, 701-794-8777 Hensler, ND 58530. Sheridan County Auditor....... 215...

  19. Go-go, 40 miljonit krooni! / Kadri Paas, Laura Birkin

    Index Scriptorium Estoniae

    Paas, Kadri, 1982-

    2007-01-01

    Majandus- ja kommunikatsiooniminister Juhan Parts tegi valitsusele ettepaneku anda maabussiliinide toetuseks veel 20 miljonit ja Edelaraudteele 21,5 miljonit krooni, Eesti Reformierakond ootab ministri selgitusi. Vt. samas: GoBus: mei ei saa bussisõidule peale maksta

  20. Principals and Vice-Principals. Bibliographies in Education No. 65.

    Science.gov (United States)

    Dohan, Margaret

    This bibliography lists material published on principals and vice-principals since March 1975. The entries include 215 books, 437 articles, and 6 theses. A list is provided of the Federation's publications in this series. (JM)

  1. 75 FR 52957 - Supplemental Funding Under the Food and Drug Administration's Food Emergency Response Laboratory...

    Science.gov (United States)

    2010-08-30

    ... HUMAN SERVICES Food and Drug Administration Supplemental Funding Under the Food and Drug Administration's Food Emergency Response Laboratory Network Microbiological Cooperative Agreement Program (U18) PAR-09-215; Request for Supplemental Applications AGENCY: Food and Drug Administration, HHS....

  2. Chemical Use for Subgrade Modification of Soils

    OpenAIRE

    Khan, Athar

    2012-01-01

    This session will discuss the use of chemicals to enhance the geotechnical behavior of subgrade material. The session will cover latest changes in INDOT specs for subgrade treatment (SEC 207), chemical modification (SEC 215) and Soils Treatment Materials (SEC 913).

  3. 78 FR 22225 - Meeting: African Development Foundation, Board of Directors Executive Session Meeting

    Science.gov (United States)

    2013-04-15

    ...; ] AFRICAN DEVELOPMENT FOUNDATION Board of Directors Executive Session Meeting Meeting: African Development Foundation, Board of Directors Executive Session Meeting Time: Tuesday, April 23, 2013 11:15 a.m. to 2:15...

  4. 75 FR 25238 - Environmental Impacts Statements; Notice of Availability

    Science.gov (United States)

    2010-05-07

    ..., Brunswick, ME, Comment Period Ends: 06/28/2010, Contact: Thomas H. Stephan, 215-897-4916. EIS No. 20100163.... EIS No. 20100164, Draft EIS, BLM, 00, Southern California Edison's Eldorado-Ivanpah Transmission...

  5. Neuroprotective role of 15-deoxy-△12,14 -prostaglandin J2 in ischelic stroke%15-脱氧-△12,14-前列腺素J2在脑缺血中的神经保护作用

    Institute of Scientific and Technical Information of China (English)

    李文惠; 刘喆

    2011-01-01

    15-deoxy-△12,14-prostaglandin J2(15d-PGJ2) is one of the natural ligands of the peroxisome proliferator-activated receptor γ.It plays an important protective role during cerebral ischemia.This article reviews the recent progress in research on the neuroprotective mechanism of 15d-PGJ2.%15-脱氧-△12,14-前列腺素J2(15-deoxy-△12,14-prostaglandin J2,15d-PGJ2)是过氧化物酶体增殖物激活受体γ的天然配体之一,在脑缺血中发挥重要的保护作用.文章对15d-PGJ2神经保护机制的研究进展进行了综述.

  6. BIOMASS, SPECIES IDENTIFICATION, SPECIES IDENTIFICATION - LIFE STAGE, TAXONOMIC CODE and species abundance tows data collected in the Gulf of Alaska on the ALPHA HELIX and WECOMA cruises HX201, HX203 and others as part of the NEP project from 1997-10-14 to 2004-10-08 (NODC Accession 0113956)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113956 includes tows and biological data collected aboard the ALPHA HELIX and WECOMA during cruises HX201, HX203, HX205, HX208, HX211, HX215, HX217,...

  7. BIOMASS, SPECIES IDENTIFICATION, TAXONOMIC CODE, SPECIES IDENTIFICATION - LIFE STAGE and species abundance tows data collected in the Gulf of Alaska on the ALPHA HELIX and WECOMA cruises HX201, HX203 and others as part of the NEP project from 1997-10-10 to 2004-10-07 (NODC Accession 0113896)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113896 includes tows and biological data collected aboard the ALPHA HELIX and WECOMA during cruises HX201, HX203, HX205, HX208, HX211, HX215, HX217,...

  8. Teachers’ individual action theories about competence-based education: the value of the cognitive apprenticeship model

    NARCIS (Netherlands)

    Seezink, Audrey; Poell, Rob; Kirschner, Paul A.

    2009-01-01

    Seezink, A., Poell, R. F., & Kirschner, P. A. (2009). Teachers' individual action theories about competence-based education: The value of the cognitive apprenticeship model. Journal of Vocational Education & Training, 61, 203-215.

  9. 77 FR 16435 - Transmission Relay Loadability Reliability Standard

    Science.gov (United States)

    2012-03-21

    ... NERC. \\44\\ 76 FR 58,424 (2011). IV. Discussion 36. Pursuant to section 215(d)(2) of the FPA, the... Reliability Standard NUC-001 (Nuclear Plant Interface Coordination) (Criteria B3).\\30\\ \\30\\ As we...

  10. 48 CFR 825.1001 - Waiver of right to examination of records.

    Science.gov (United States)

    2010-10-01

    ..., Contract Terms and Conditions Required to Implement Statutes or Executive Orders—Commercial Items. (2) Alternate III of the FAR clause at 52.215-2, Audit and Records—Negotiation. (b) The contracting officer...

  11. Libraries in Pennsylvania: MedlinePlus

    Science.gov (United States)

    ... Doylestown Doylestown Hospital Medical Library 595 West State Street Doylestown, PA 18901 215-345-2606 http://www.dh.org/MedicalLibrary Enola National Sexual Violence Resource Center NSVRC Library 123 North Enola Drive ...

  12. USA terrorismivastane seadus ja jälitustegevuse erivolitused / Piibe Jõgi

    Index Scriptorium Estoniae

    Jõgi, Piibe, 1969-

    2007-01-01

    24. okt. 2001 Ameerika Ühendriikides vastu võetud terrorismivastase seaduse (nn USA Patriot Act) paragrahvist 215, mis käsitleb andmete salajast kogumist ja paragrahvist 213 salajaste läbiotsimisite kohta

  13. 78 FR 45592 - Reporting and Recordkeeping Requirements Under OMB Review

    Science.gov (United States)

    2013-07-29

    ...: Small Business Lending Companies. Responses: 215. Annual Burden: 107.50. Curtis Rich, Management Analyst... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Reporting and Recordkeeping Requirements Under OMB Review AGENCY: Small Business...

  14. 31 CFR 535.333 - Properties.

    Science.gov (United States)

    2010-07-01

    ....333 Properties. (a) The term properties as used in § 535.215 means all uncontested and non-contingent liabilities and property interests of the Government of Iran, its agencies, instrumentalities, or...

  15. 78 FR 43709 - Affirmatively Furthering Fair Housing

    Science.gov (United States)

    2013-07-19

    ... prevent the increase of segregation, in ghettos, of racial groups whose lack of opportunity the Act was... Multilevel Approach Am. J. Public Health Vol. 93(2) p. 215-221 (Feb. 2003) available at...

  16. 78 FR 17917 - Medical Waivers for Merchant Mariner Credential Applicants With a History of Seizure Disorders

    Science.gov (United States)

    2013-03-25

    ... Federal Register (73 FR 3316). Background and Purpose Coast Guard regulations in 46 CFR 10.215 contain the... penetrating head injury; (b) Intracerebral hemorrhage of any etiology, including stroke and trauma; (c)...

  17. 48 CFR 570.604 - Deviations to provisions and clauses.

    Science.gov (United States)

    2010-10-01

    ... Contingent Fees, FAR 52.215-2, Audit and Records—Negotiation). The authorizing statue must allow for a waiver. (c) Certain clauses required by non-GSA regulations require approval of the issuing agency before...

  18. Transformation of Actinomyces spp. by a gram-negative broad-host-range plasmid.

    OpenAIRE

    Yeung, M K; Kozelsky, C S

    1994-01-01

    The gram-negative broad-host-range vector pJRD215 was transferred by electroporation into strains of Actinomyces viscosus or Actinomyces naeslundii at efficiencies which ranged from 10(2) to 10(7) transformants per microgram of plasmid DNA. The Actinomyces transformants expressed pJRD215-encoded resistance to kanamycin and streptomycin. Moreover, the transforming plasmid DNA had not undergone any deletions or rearrangements, nor had it integrated into the genomes of these strains.

  19. Evaluation scheme for Blatta orientalis and Blattella germanica in food units with HACCP implementation

    OpenAIRE

    VINELA REFUGJATI; BIZENA BIJO; ENDRI HAXHIRAJ

    2014-01-01

    Blattella germanica and Blatta orientalis are the most common insects found in food industry, houses, offices, hotels, schools, etc. The aim of this study is the evaluation of gel baits efficiency (2.15% imidacloprid) against Blatta orientalis and Blattella germanica in albanian food units. The study was carried out in Tirana from June 2012 to June 2013 in 14 infested supermarkets, 13 of which were treated with 2.15% imidacloprid while 1 served as control unit. Monitor traps and visual inspec...

  20. Exploratory experimental investigation of a wave propeller

    OpenAIRE

    Dane, Carl W.

    1992-01-01

    Approved for public release; distribution is unlimited A low-speed wind tunnel investigation was conducted to determine if a small secondary airfoil or wave propeller, oscillating in a rotary plunging motion, could significantly affect the airflow over a lifting airfoil surface to delay the onset of stall. The lifting airfoil shape was a NACA 66(215)-216, chosen for its chordwise pressure port instrumentation. Testing consisted of measuring the pressure distribution of the NACA 66(215)-2...

  1. Minor Actinide Burning in Thermal Reactors. A Report by the Working Party on Scientific Issues of Reactor Systems

    International Nuclear Information System (INIS)

    The actinides (or actinoids) are those elements in the periodic table from actinium upwards. Uranium (U) and plutonium (Pu) are two of the principal elements in nuclear fuel that could be classed as major actinides. The minor actinides are normally taken to be the triad of neptunium (Np), americium (Am) and curium (Cm). The combined masses of the remaining actinides (i.e. actinium, thorium, protactinium, berkelium, californium, einsteinium and fermium) are small enough to be regarded as very minor trace contaminants in nuclear fuel. Those elements above uranium in the periodic table are known collectively as the transuranics (TRUs). The operation of a nuclear reactor produces large quantities of irradiated fuel (sometimes referred to as spent fuel), which is either stored prior to eventual deep geological disposal or reprocessed to enable actinide recycling. A modern light water reactor (LWR) of 1 GWe capacity will typically discharge about 20-25 tonnes of irradiated fuel per year of operation. About 93-94% of the mass of uranium oxide irradiated fuel is comprised of uranium (mostly 238U), with about 4-5% fission products and ∼1% plutonium. About 0.1-0.2% of the mass is comprised of neptunium, americium and curium. These latter elements accumulate in nuclear fuel because of neutron captures, and they contribute significantly to decay heat loading and neutron output, as well as to the overall radio-toxic hazard of spent fuel. Although the total minor actinide mass is relatively small - approximately 20-25 kg per year from a 1 GWe LWR - it has a disproportionate impact on spent fuel disposal, and thus the longstanding interest in transmuting these actinides either by fission (to fission products) or neutron capture in order to reduce their impact on the back end of the fuel cycle. The combined masses of the trace actinides actinium, thorium, protactinium, berkelium and californium in irradiated LWR fuel are only about 2 parts per billion, which is far too low for

  2. TRASH TO TREASURE: CONVERTING COLD WAR LEGACY WASTE INTO WEAPONS AGAINST CANCER

    International Nuclear Information System (INIS)

    As part of its commitment to clean up Cold War legacy sites, the U.S. Department of Energy (DOE) has initiated an exciting and unique project to dispose of its inventory of uranium-233 (233U) stored at Oak Ridge National Laboratory (ORNL), and extract isotopes that show great promise in the treatment of deadly cancers. In addition to increasing the supply of potentially useful medical isotopes, the project will rid DOE of a nuclear concern and cut surveillance and security costs. For more than 30 years, DOE's ORNL has stored over 1,200 containers of fissile 233U, originally produced for several defense-related projects, including a pilot study that looked at using 233U as a commercial reactor fuel. This uranium, designated as special nuclear material, requires expensive security, safety, and environmental controls. It has been stored at an ORNL facility, Building 3019A, that dates back to the Manhattan Project. Down-blending the material to a safer form, rather than continuing to store it, will eliminate a $15 million a year financial liability for the DOE and increase the supply of medical isotopes by 5,700 percent. During the down-blending process, thorium-229 (229Th) will be extracted. The thorium will then be used to extract actinium-225 (225Ac), which will ultimately supply its progeny, bismuth-213 (213Bi), for on-going cancer research. The research includes Phase II clinical trials for the treatment of acute myelogenous leukemia at Sloan-Kettering Memorial Cancer Center in New York, as well as other serious cancers of the lungs, pancreas, and kidneys using a technique known as alpha-particle radioimmunotherapy. Alpha-particle radioimmunotherapy is based on the emission of alpha particles by radionuclides. 213Bi is attached to a monoclonal antibody that targets specific cells. The bismuth then delivers a high-powered but short-range radiation dose, effectively killing the cancerous cells but sparing the surrounding tissue. Production of the actinium and

  3. TRASH TO TREASURE: CONVERTING COLD WAR LEGACY WASTE INTO WEAPONS AGAINST CANCER

    Energy Technology Data Exchange (ETDEWEB)

    Nicholas, R.G.; Lacy, N.H.; Butz, T.R.; Brandon, N.E.

    2004-10-06

    As part of its commitment to clean up Cold War legacy sites, the U.S. Department of Energy (DOE) has initiated an exciting and unique project to dispose of its inventory of uranium-233 (233U) stored at Oak Ridge National Laboratory (ORNL), and extract isotopes that show great promise in the treatment of deadly cancers. In addition to increasing the supply of potentially useful medical isotopes, the project will rid DOE of a nuclear concern and cut surveillance and security costs. For more than 30 years, DOE's ORNL has stored over 1,200 containers of fissile 233U, originally produced for several defense-related projects, including a pilot study that looked at using 233U as a commercial reactor fuel. This uranium, designated as special nuclear material, requires expensive security, safety, and environmental controls. It has been stored at an ORNL facility, Building 3019A, that dates back to the Manhattan Project. Down-blending the material to a safer form, rather than continuing to store it, will eliminate a $15 million a year financial liability for the DOE and increase the supply of medical isotopes by 5,700 percent. During the down-blending process, thorium-229 (229Th) will be extracted. The thorium will then be used to extract actinium-225 (225Ac), which will ultimately supply its progeny, bismuth-213 (213Bi), for on-going cancer research. The research includes Phase II clinical trials for the treatment of acute myelogenous leukemia at Sloan-Kettering Memorial Cancer Center in New York, as well as other serious cancers of the lungs, pancreas, and kidneys using a technique known as alpha-particle radioimmunotherapy. Alpha-particle radioimmunotherapy is based on the emission of alpha particles by radionuclides. 213Bi is attached to a monoclonal antibody that targets specific cells. The bismuth then delivers a high-powered but short-range radiation dose, effectively killing the cancerous cells but sparing the surrounding tissue. Production of the actinium and

  4. TAM类突变对B'亚型HIV病毒耐药和复制能力的影响%The impact of thymidine analogue resistance mutations on the level of drug resistance and replicative capacity of HIV-1 subtype B' viruses

    Institute of Scientific and Technical Information of China (English)

    周茜; 邢辉; 方志明; 陈彬; 王铮; 邵一鸣; 廖玲洁; 黄汉菊

    2013-01-01

    Objective To study the influence of thymidine analogue resistance mutations (TAMs) such as T215Y and L210W on antiretroviral drug resistance and replicative capacity of HIV-1 viruses under the genetic background of pol fragment of subtype B' strains.Methods The plasma sample was collected from a patient infected with HIV-1 subtype B' strains who was under antiretroviral therapy and with TAM mutations.Partial pol gene was amplified from three different viral quasispecies with single genome amplification (SGA),T215Y and L210W was reverse mutated to wild type codons by PCR based point mutation.Recombinant infectious clones were constructed by replacing pol fragment of pNL4-3-BstⅡ with counterparts from the patient's viruses.The level of drug resistance and replicative dynamics were evaluated via luciferase assay and P24 measurement.Results Three recombinant infectious clones were successfully constructed,named as pNL-PAT-1,pNL-PAT-2 and pNL-PAT-3 with mutations T215Y,M41L/L210W/T215Y and M41L/L210W/T215Y,respectively.Three corresponding infectious clones with reverse mutations at 215 and 201 positions were pNL-PAT-1-T215Y (-),pNL-PAT-2-L210W(-) and pNL-PAT-3-L210W(-).Compared with the IC50 values to AZT in the original viruses,those in the mutated viruses had been lowered for 4.9,8.1 and 2.7 times.pNL-PAT-1-T215Y(-) had a higher level of P24 in supernatant than pNL-PAT-1 did.The viruses with reverse mutation of L210W had different levels of replication as compared with the original ones:pNL-PAT-2-L210W(-) had a slightly lower level of P24 than pNL-PAT-2 did,but pNL-PAT-3-L210W(-) had a much higher level of P24 as compared with pNL-PAT-3.Conclusions TAM mutations such as T215Y and L210W can raise the resistance to AZT of subtype B' strains to certain levels.T215Y can impair viral replicative fitness.However,L210W might have different impacts on replicative capacity in different HIV-1 strains.Genetic diversity may have a role in drug resistance and viral replicative

  5. Formerly utilized MED/AEC sites remedial action program. Radiological survey of the E. I. Du Pont de Nemours and Co. , Deepwater, New Jersey. Final report

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-12-01

    The results of a radiological survey of the E. I. Du Pont Company, Deepwater, New Jersey, are presented in this report. During the 1940's this site was used for development of a process for the conversion of uranium oxide to produce uranium tetrafluoride and small quantities of uranium metal. The survey included measurements of the following: residual alpha and beta--gamma contamination levels, both fixed and transferable, in the one remaining uranium tetrafluoride operations building; external gamma radiation levels at 1 m above the surface inside this building and at outdoor locations where radioactive materials were handled; radon and radon daughter concentrations in the air in the operations building; uranium, radium, actinium, and thorium concentrations in soil and water on and near the site; and the airborne concentrations of uranium, thorium, and radium in the operations building. Elevated concentrations of uranium were found in residues from the operations building and in some surface and subsurface soil samples. Alpha and beta--gamma contamination levels in some areas of the operations building were above the limits of current federal guidelines set for the release of property for unrestricted use. 18 figures, 11 tables.

  6. Ac, La, and Ce radioimpurities in {sup 225}Ac produced in 40-200 MeV proton irradiations of thorium

    Energy Technology Data Exchange (ETDEWEB)

    Engle, Jonathan W.; Ballard, Beau D. [Los Alamos National Laboratory, NM (United States); Weidner, John W. [Air Force Institute of Technology, Wright Patterson Air Force Base, OH (United States); and others

    2014-10-01

    Accelerator production of {sup 225}Ac addresses the global supply deficiency currently inhibiting clinical trials from establishing {sup 225}Ac's therapeutic utility, provided that the accelerator product is of sufficient radionuclidic purity for patient use. Two proton activation experiments utilizing the stacked foil technique between 40 and 200 MeV were employed to study the likely co-formation of radionuclides expected to be especially challenging to separate from {sup 225}Ac. Foils were assayed by nondestructive γ-spectroscopy and by α-spectroscopy of chemically processed target material. Nuclear formation cross sections for the radionuclides {sup 226}Ac and {sup 227}Ac as well as lower lanthanide radioisotopes {sup 139}Ce, {sup 141}Ce, {sup 143}Ce, and {sup 140}La whose elemental ionic radii closely match that of actinium were measured and are reported. The predictions of the latest MCNP6 event generators are compared with measured data, as they permit estimation of the formation rates of other radionuclides whose decay emissions are not clearly discerned in the complex spectra collected from {sup 232}Th(p,x) fission product mixtures. (orig.)

  7. Pulse-shape discrimination and energy quenching of alpha particles in Cs$_2$LiLaBr$_6$:Ce$^{3+}$

    CERN Document Server

    Mesick, Katherine E; Stonehill, Laura C

    2016-01-01

    Cs$_2$LiLaBr$_6$:Ce$^{3+}$ (CLLB) is an elpasolite scintillator that offers excellent linearity and gamma-ray energy resolution and sensitivity to thermal neutrons with the ability to perform pulse-shape discrimination (PSD) to distinguish gammas and neutrons. Our investigation of CLLB has indicated the presence of intrinsic radioactive alpha background that we have determined to be from actinium contamination of the lanthanum component. We measured the pulse shapes for gamma, thermal neutron, and alpha events and determined that PSD can be performed to separate the alpha background with a moderate figure of merit of 0.98. We also measured the electron-equivalent-energy of the alpha particles in CLLB and simulated the intrinsic alpha background from $^{227}$Ac to determine the quenching factor of the alphas. A linear quenching relationship $L_{\\alpha} = E_{\\alpha} \\times q + L_0$ was found at alpha particle energies above 5 MeV, with a quenching factor $q = 0.71$ MeVee/MeV and an offset $L_0 = - 1.19$ MeVee.

  8. An Ion Exchange Study of Possible Hydridized 5f Bonding in theActinides

    Energy Technology Data Exchange (ETDEWEB)

    Diamond, R.M.; Street, Jr., K.; Seaborg, G.T.

    1951-08-28

    A study has been made of the elution behavior of curium(III), americium(III), plutonium(III), actinium(III), plutonium(IV), neptunium(IV), uraniuM(IV), thorium(IV), neptunium(V), plutonium (VI), uranium (VI), lanthanum(III), cerium(III), europium(III), ytterbium(III), ytterium(III), strontium(II), barium(II), radium(II), cesium(I) with 3.2 M, 6.2 M, 9.3 M, and 12.2 M HCl solutions from Dowex-50 cation exchange resin columns. These elutions show that in high concentrations of hydrochloric acid the actinides form complex ions with chloride ion to a much greater extent than the lanthanides. The strengths of the tripositive actinide complex ions apparently go in the order plutonium > americium> curium, although their ionic radii also decrease in this same order. To explain these results, a partial covalent character may be ascribed to the bonding in the transuranium complex ions. It is shown that a reasonable structure for such covalent bonding involves hybridization of the 5f orbitals in the actinide elements.

  9. Extending the north-east limit of the chart of nuclides

    CERN Document Server

    Benlliure, J; Kurtukian-Nieto, T; Schmidt, K -H; Audouin, L; Blank, B; Becker, F; Casarejos, E; Cortina-Gil, D; Enqvist, T; Fernández, B; Fernández-Ordóñez, M; Giovinazzo, J; Henzlova, D; Junghans, A R; Jurado, B; Napolitani, P; Pereira, J; Rejmund, F; Yordanov, O

    2010-01-01

    The existence of nuclei with exotic combinations of protons and neutrons provides fundamental information on the forces acting between nucleons. The maximum number of neutrons a given number of protons can bind, neutron drip line1, is only known for the lightest chemical elements, up to oxygen. For heavier elements, the larger its atomic number, the farther from this limit is the most neutron-rich known isotope. The properties of heavy neutron-rich nuclei also have a direct impact on understanding the observed abundances of chemical elements heavier than iron in our Universe. Above half of the abundances of these elements are thought to be produced in rapid-neutron capture reactions, r-process, taking place in violent stellar scenarios2 where heavy neutron-rich nuclei, far beyond the ones known up today, are produced. Here we present a major step forward in the production of heavy neutron-rich nuclei: the discovery of 73 new neutron-rich isotopes of chemical elements between tantalum (Z=72) and actinium (Z=89...

  10. Liquid Scintillation Counting of Environmental Radioisotopes: A Review of the Impact of Background Reduction

    Energy Technology Data Exchange (ETDEWEB)

    Douglas, Matthew; Bernacki, Bruce E.; Erchinger, Jennifer L.; Finn, Erin C.; Fuller, Erin S.; Hoppe, Eric W.; Keillor, Martin E.; Morley, Shannon M.; Mullen, Crystal A.; Orrell, John L.; Panisko, Mark E.; Warren, Glen A.; Wright, Michael E.

    2016-03-09

    Liquid scintillation counting (LSC) is a versatile and commonplace method for radiometric measurement of charged particle emitting radionuclides. The LSC method provides utility in a range of environmental science applications including hydrological studies of water transport, anthropogenic releases of radionuclides into the environment, and vertical mixing rates within oceans. Instrumental measurement background is one limiting factor of radiometric measurement sensitivity. As part of the development of a custom low background LSC system located in a shallow underground laboratory at Pacific Northwest National Laboratory, a number of measurement applications of LSC have been considered and are summarized here. The focus is on determining which aspects of such measurements would gain the greatest benefit from the reduction of LSC backgrounds by a factor of 10-100 relative to values reported in the literature. Examples of benefits include lowering the minimum detectable activity, reducing the sample size required, and shortening the elapsed timeline of the processing and analysis sequence. In particular tritium, strontium, and actinium isotopes are examined as these isotopes cover a range of requirements related to the LSC measurement method (e.g., 3H: low energy; Sr: spectral deconvolution; Ac: alpha/beta discrimination).

  11. Development of an Ultra-Low Background Liquid Scintillation Counter for Trace Level Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Erchinger, Jennifer L.; Orrell, John L.; Aalseth, Craig E.; Bernacki, Bruce E.; Douglas, Matthew; Finn, Erin C.; Fuller, Erin S.; Keillor, Martin E.; Morley, Shannon M.; Mullen, Crystal A.; Panisko, Mark E.; Shaff, Sarah M.; Warren, Glen A.; Wright, Michael E.

    2015-09-01

    Low-level liquid scintillation counting (LSC) has been established as one of the radiation detection techniques useful in elucidating environmental processes and environmental monitoring around nuclear facilities. The Ultra-Low Background Liquid Scintillation Counter (ULB-LSC) under construction in the Shallow Underground Laboratory at Pacific Northwest National Laboratory aims to further reduce the MDAs and/or required sample processing. Through layers of passive shielding in conjunction with an active veto and 30 meters water equivalent overburden, the background reduction is expected to be 10 to 100 times below typical analytic low-background liquid scintillation systems. Simulations have shown an expected background of around 14 counts per day. A novel approach to the light collection will use a coated hollow light guide cut into the inner copper shielding. Demonstration LSC measurements will show low-energy detection, spectral deconvolution, and alpha/beta discrimination capabilities, from trials with standards of tritium, strontium-90, and actinium-227, respectively. An overview of the system design and expected demonstration measurements will emphasize the potential applications of the ULB-LSC in environmental monitoring for treaty verification, reach-back sample analysis, and facility inspections.

  12. Purification of lanthanides for double beta decay experiments

    Energy Technology Data Exchange (ETDEWEB)

    Polischuk, O. G. [Institute for Nuclear Research, MSP 03680 Kyiv, Ukraine and INFN, Section of Rome, La Sapienza, I-00185 Rome (Italy); Barabash, A. S. [Institute of Theoretical and Experimental Physics, 117259 Moscow (Russian Federation); Belli, P. [INFN, Section of Rome Tor Vergata, I-00133 Rome (Italy); Bernabei, R. [INFN, Section of Rome Tor Vergata, I-00133 Rome, Italy and Department of Physics, University of Rome Tor Vergata, I-00133 Rome (Italy); Boiko, R. S.; Danevich, F. A.; Mokina, V. M.; Poda, D. V.; Tretyak, V. I. [Institute for Nuclear Research, MSP 03680 Kyiv (Ukraine); Cappella, F.; Incicchitti, A. [INFN, Section of Rome La Sapienza, I-00185 Rome, Italy and Department of Physics, University of Rome La Sapienza, I-00185 Rome (Italy); Cerulli, R.; Laubenstein, M.; Nisi, S. [INFN, Gran Sasso National Laboratories, I-67100 Assergi (Aq) (Italy)

    2013-08-08

    There are several potentially double beta active isotopes among the lanthanide elements. However, even high purity grade lanthanide compounds contain {sup 238}U, {sup 226}Ra and {sup 232,228}Th typically on the level of ∼ (0.1 - 1) Bq/kg. The liquid-liquid extraction technique was used to remove traces of U, Ra and Th from CeO{sub 2}, Nd{sub 2}O{sub 3} and Gd{sub 2}O{sub 3}. The radioactive contamination of the samples before and after the purification was tested by using ultra-low-background HPGe γ spectrometry at the underground Gran Sasso National Laboratories of the INFN (Italy). After the purification the radioactive contamination of gadolinium oxide by Ra and Th was decreased at least one order of magnitude. The efficiency of the approach to purify cerium oxide from Ra was on same level, while the radioactive contamination of neodymium sample before and after the purification is below the sensitivity of analytical methods. The purification method is much less efficient for chemically very similar radioactive elements like lanthanum, lutetium and actinium. R and D of the methods to remove the pollutions with improved efficiency is in progress.

  13. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin.

    Science.gov (United States)

    Nour, S; El-Sharkawy, A; Burnett, W C; Horwitz, E P

    2004-12-01

    The objective of this work was to establish a new procedure for 228Ra determination of natural waters via preconcentration of radium on MnO2 and separation of its daughter, 228Ac, using Diphonix ion exchange resin. Following removal of potential interferences via passage through an initial Diphonix Resin column, the first daughter of 228Ra, 228Ac, is isolated by chromatographic separation via a second Diphonix column. A holding time of > 30 h for 228Ac ingrowth in between the two column separations ensures secular equilibrium. Barium-133 is used as a yield tracer. Actinium-228 is eluted from the second Diphonix Resin with 5 ml 1M 1-Hydroxyethane-1,1-diphosphonic acid (HEDPA) and quantified by addition of scintillation cocktail and LSC counting. Radium (and 133Ba) from the load and rinse solutions from the 2nd Diphonix column may be prepared for alpha spectrometry (for determination of 223Ra, 224Ra, and 226Ra) by BaSO4 microprecipitation and filtration. Decontamination tests indicate that U, Th, and Ra series nuclides do not interfere with these measurements, although high contents of 90Sr (90Y) require additional treatment for accurate measurement of 228Ra. Addition of stable Sr as a "hold back" carrier during the initial MnO2 preconcentration step was shown to remove most 90Sr interference.

  14. Soil and river sediments radionuclides monitoring at Aramar Experimental Center: an historical overview

    Energy Technology Data Exchange (ETDEWEB)

    Segre, Nadia; Fagundes, Rosane Correa, E-mail: nadia.segre@ctmsp.mar.mil.br [Centro Tecnologico da Marinha (CTM-SP/CEA/LARE), Ipero, SP (Brazil). Centro Experimental Aramar. Lab. Radioecologico; Moraes, Marco Antonio P.V. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    In order to evaluate possible effects to the environment resulting from the implementation of the Centro Tecnologico da Marinha - Centro Experimental Aramar (CTMSP-CEA) at Ipero in Sao Paulo state, Brazil, which came into operation in 1989, an Environmental Monitoring Program (PMA) was established in October, 1987. One of the aims of this program is to monitor the soil and river sediments radionuclides levels at CEA and beyond its boundary. The utilization of statistical tools to evaluate the results of radiometric environmental monitoring is a procedure required by National Nuclear Energy Commission (CNEN). The box plot is a simple statistical tool for displaying data. The central tendency and dispersion of the results as well as the observation of unusual results (outliers) in the dataset are easily visualized. Control chart is a graph that maps data and provides a picture of how a process is performing over time. A control chart always has a central line for the mean, an upper line for the upper control limit and a lower line for the lower control limit. Box plots and control charts were used to visualize the annual amount of natural uranium, lead-214, actinium-228 and lead-212 in soil and river sediment detected between 1987 and 2011, considering the measurements of all monitored places each year. This historical observation shows that, in average, the results obtained are below than the 1987-1988 levels (CEA's pre-operational) or below than the backgrounds radionuclides values. (author)

  15. Background radiation and individual dosimetry in the costal area of Tamil Nadu, India.

    Science.gov (United States)

    Matsuda, Naoki; Brahmanandhan, G M; Yoshida, Masahiro; Takamura, Noboru; Suyama, Akihiko; Koguchi, Yasuhiro; Juto, Norimichi; Raj, Y Lenin; Winsley, Godwin; Selvasekarapandian, S

    2011-07-01

    South coast of India is known as the high-level background radiation area (HBRA) mainly due to beach sands that contain natural radionuclides as components of the mineral monazite. The rich deposit of monazite is unevenly distributed along the coastal belt of Tamil Nadu and Kerala. An HBRA site that laid in 2×7 m along the sea was found in the beach of Chinnavillai, Tamil Nadu, where the maximum ambient dose equivalent reached as high as 162.7 mSv y(-1). From the sands collected at the HBRA spot, the high-purity germanium semi-conductor detector identified six nuclides of thorium series, four nuclides of uranium series and two nuclides belonging to actinium series. The highest radioactivity observed was 43.7 Bq g(-1) of Th-228. The individual dose of five inhabitants in Chinnavillai, as measured by the radiophotoluminescence glass dosimetry system, demonstrated the average dose of 7.17 mSv y(-1) ranging from 2.79 to 14.17 mSv y(-1).

  16. DISSOLVED CONCENTRATION LIMITS OF RADIOACTIVE ELEMENTS

    Energy Technology Data Exchange (ETDEWEB)

    NA

    2004-11-22

    The purpose of this study is to evaluate dissolved concentration limits (also referred to as solubility limits) of elements with radioactive isotopes under probable repository conditions, based on geochemical modeling calculations using geochemical modeling tools, thermodynamic databases, field measurements, and laboratory experiments. The scope of this modeling activity is to predict dissolved concentrations or solubility limits for 14 elements with radioactive isotopes (actinium, americium, carbon, cesium, iodine, lead, neptunium, plutonium, protactinium, radium, strontium, technetium, thorium, and uranium) important to calculated dose. Model outputs for uranium, plutonium, neptunium, thorium, americium, and protactinium are in the form of tabulated functions with pH and log (line integral) CO{sub 2} as independent variables, plus one or more uncertainty terms. The solubility limits for the remaining elements are either in the form of distributions or single values. The output data from this report are fundamental inputs for Total System Performance Assessment for the License Application (TSPA-LA) to determine the estimated release of these elements from waste packages and the engineered barrier system. Consistent modeling approaches and environmental conditions were used to develop solubility models for all of the actinides. These models cover broad ranges of environmental conditions so that they are applicable to both waste packages and the invert. Uncertainties from thermodynamic data, water chemistry, temperature variation, and activity coefficients have been quantified or otherwise addressed.

  17. A Critical Review of Alpha Radionuclide Therapy-How to Deal with Recoiling Daughters?

    Science.gov (United States)

    de Kruijff, Robin M; Wolterbeek, Hubert T; Denkova, Antonia G

    2015-01-01

    This review presents an overview of the successes and challenges currently faced in alpha radionuclide therapy. Alpha particles have an advantage in killing tumour cells as compared to beta or gamma radiation due to their short penetration depth and high linear energy transfer (LET). Touching briefly on the clinical successes of radionuclides emitting only one alpha particle, the main focus of this article lies on those alpha-emitting radionuclides with multiple alpha-emitting daughters in their decay chain. While having the advantage of longer half-lives, the recoiled daughters of radionuclides like 224Ra (radium), 223Ra, and 225Ac (actinium) can do significant damage to healthy tissue when not retained at the tumour site. Three different approaches to deal with this problem are discussed: encapsulation in a nano-carrier, fast uptake of the alpha emitting radionuclides in tumour cells, and local administration. Each approach has been shown to have its advantages and disadvantages, but when larger activities need to be used clinically, nano-carriers appear to be the most promising solution for reducing toxic effects, provided there is no accumulation in healthy tissue. PMID:26066613

  18. A Critical Review of Alpha Radionuclide Therapy—How to Deal with Recoiling Daughters?

    Science.gov (United States)

    de Kruijff, Robin M.; Wolterbeek, Hubert T.; Denkova, Antonia G.

    2015-01-01

    This review presents an overview of the successes and challenges currently faced in alpha radionuclide therapy. Alpha particles have an advantage in killing tumour cells as compared to beta or gamma radiation due to their short penetration depth and high linear energy transfer (LET). Touching briefly on the clinical successes of radionuclides emitting only one alpha particle, the main focus of this article lies on those alpha-emitting radionuclides with multiple alpha-emitting daughters in their decay chain. While having the advantage of longer half-lives, the recoiled daughters of radionuclides like 224Ra (radium), 223Ra, and 225Ac (actinium) can do significant damage to healthy tissue when not retained at the tumour site. Three different approaches to deal with this problem are discussed: encapsulation in a nano-carrier, fast uptake of the alpha emitting radionuclides in tumour cells, and local administration. Each approach has been shown to have its advantages and disadvantages, but when larger activities need to be used clinically, nano-carriers appear to be the most promising solution for reducing toxic effects, provided there is no accumulation in healthy tissue. PMID:26066613

  19. A Critical Review of Alpha Radionuclide Therapy—How to Deal with Recoiling Daughters?

    Directory of Open Access Journals (Sweden)

    Robin M. de Kruijff

    2015-06-01

    Full Text Available This review presents an overview of the successes and challenges currently faced in alpha radionuclide therapy. Alpha particles have an advantage in killing tumour cells as compared to beta or gamma radiation due to their short penetration depth and high linear energy transfer (LET. Touching briefly on the clinical successes of radionuclides emitting only one alpha particle, the main focus of this article lies on those alpha-emitting radionuclides with multiple alpha-emitting daughters in their decay chain. While having the advantage of longer half-lives, the recoiled daughters of radionuclides like 224Ra (radium, 223Ra, and 225Ac (actinium can do significant damage to healthy tissue when not retained at the tumour site. Three different approaches to deal with this problem are discussed: encapsulation in a nano-carrier, fast uptake of the alpha emitting radionuclides in tumour cells, and local administration. Each approach has been shown to have its advantages and disadvantages, but when larger activities need to be used clinically, nano-carriers appear to be the most promising solution for reducing toxic effects, provided there is no accumulation in healthy tissue.

  20. Tumor Immunotargeting Using Innovative Radionuclides

    Directory of Open Access Journals (Sweden)

    Françoise Kraeber-Bodéré

    2015-02-01

    Full Text Available This paper reviews some aspects and recent developments in the use of antibodies to target radionuclides for tumor imaging and therapy. While radiolabeled antibodies have been considered for many years in this context, only a few have reached the level of routine clinical use. However, alternative radionuclides, with more appropriate physical properties, such as lutetium-177 or copper-67, as well as alpha-emitting radionuclides, including astatine-211, bismuth-213, actinium-225, and others are currently reviving hopes in cancer treatments, both in hematological diseases and solid tumors. At the same time, PET imaging, with short-lived radionuclides, such as gallium-68, fluorine-18 or copper-64, or long half-life ones, particularly iodine-124 and zirconium-89 now offers new perspectives in immuno-specific phenotype tumor imaging. New antibody analogues and pretargeting strategies have also considerably improved the performances of tumor immunotargeting and completely renewed the interest in these approaches for imaging and therapy by providing theranostics, companion diagnostics and news tools to make personalized medicine a reality.

  1. Tumor immunotargeting using innovative radionuclides.

    Science.gov (United States)

    Kraeber-Bodéré, Françoise; Rousseau, Caroline; Bodet-Milin, Caroline; Mathieu, Cédric; Guérard, François; Frampas, Eric; Carlier, Thomas; Chouin, Nicolas; Haddad, Ferid; Chatal, Jean-François; Faivre-Chauvet, Alain; Chérel, Michel; Barbet, Jacques

    2015-01-01

    This paper reviews some aspects and recent developments in the use of antibodies to target radionuclides for tumor imaging and therapy. While radiolabeled antibodies have been considered for many years in this context, only a few have reached the level of routine clinical use. However, alternative radionuclides, with more appropriate physical properties, such as lutetium-177 or copper-67, as well as alpha-emitting radionuclides, including astatine-211, bismuth-213, actinium-225, and others are currently reviving hopes in cancer treatments, both in hematological diseases and solid tumors. At the same time, PET imaging, with short-lived radionuclides, such as gallium-68, fluorine-18 or copper-64, or long half-life ones, particularly iodine-124 and zirconium-89 now offers new perspectives in immuno-specific phenotype tumor imaging. New antibody analogues and pretargeting strategies have also considerably improved the performances of tumor immunotargeting and completely renewed the interest in these approaches for imaging and therapy by providing theranostics, companion diagnostics and news tools to make personalized medicine a reality. PMID:25679452

  2. Rutherford's curriculum vitae, 1894-1907.

    Science.gov (United States)

    Cohen, M

    1995-06-01

    A single page, handwritten document was discovered when the Macdonald Physics building of McGill University in Montreal was gutted in 1978. This proved to be the draft of Ernest Rutherford's curriculum vitae (C.V.) covering the years 1894-1907, probably written in the autumn of 1906 when Rutherford was preparing to leave McGill. The C.V. contains 21 headings in chronological order, referring to research and other activities of Rutherford and his coauthors (especially Soddy and Barnes), plus a further set of headings relating to the associated investigations of Rutherford's team, including Eve and Hahn. A transcript of the document is provided, although in several places, Rutherford's handwriting is difficult to interpret, and the significance of his abbreviations is not always clear. Each of the items in the C.V. is discussed briefly in this review, in the light both of Rutherford's personal career and of the contribution of his team to the development and understanding of radioactivity. This contribution included the cause and nature of radioactivity (with Soddy), energy aspects of radioactive decay (with Barnes), elucidation of the uranium-radium, thorium and actinium series (Godlewski and Hahn), the radioactivity of the earth and atmosphere (Eve), the nature of the gamma rays (Eve) and, perhaps most important of all, the nature and properties of the alpha particle (Rutherford himself). The latter investigations led directly to Rutherford's later work in Manchester, including the nuclear model of the atom and artificial disintegration of the nucleus.

  3. An alternative transformation in ranking using l_1 -norm in data envelopment analysis

    Science.gov (United States)

    Ziari, S.

    2016-05-01

    Jahanshahloo et al. (Appl Math Comput 153:215-224, 2004) propose a method for ranking extremely efficient decision making units (DMUs) in data envelopment analysis (DEA) using super-efficiency technique and l1-norm and they show that the presented method is able to eliminate the existing difficulties in some methods. This paper suggests an alternative transformation to convert the nonlinear model proposed by Jahanshahloo et al. (Appl Math Comput 153:215-224, 2004) into a linear programming form. The present paper shows that model with this transformation is equivalent to the above-mentioned nonlinear model. The motivation of this work is to linearize the proposed nonlinear model by Jahanshahloo et al. (Appl Math Comput 153:215-224, 2004) which has the higher order of complexity.

  4. [Prevalence of transmission of zidovudine-resistant viruses in Switzerland. l'Etude suisse de cohorte VIH].

    Science.gov (United States)

    Yerly, S; Rakik, A; Kinloch-de-Loes, S; Erb, P; Vernazza, P; Hirschel, B; Perrin, L

    1996-10-26

    Zidovudine (ZDV) was the most widely used anti-HIV drug between 1987 and 1995, and, as already reported, transmission of ZDV-resistant viruses occurs. Several mutations of the reverse transcriptase gene have been identified; one of them affects the 215 codon and is associated with a high degree of resistance. We have determined, using selective PCR, the prevalence of transmission of 215 mutant isolates in 134 patients with primary HIV infection (PHI) and have identified 8 patients with 215 mutant virus between 1989 and 1995 in Switzerland. Mutant resistant viruses have been isolated from patients treated with most antiviral drugs. A systematic search for mutant viruses may provide useful information for the adaptation of treatment strategies. PMID:9005525

  5. Clinical analysis of chemotherapy on secondary diabetes of breast cancer patients treated with taxanes agent%紫杉类化疗方案治疗乳腺癌继发糖尿病的相关分析

    Institute of Scientific and Technical Information of China (English)

    徐方贵; 吴德柱; 王剑平; 杜京丽; 支波波; 林雨冬

    2014-01-01

    Objective:To observate the affect of taxanes agent chemotheraPy on the blood glucose of Patients with breast cancer. Methods:The data of 215 breast cancer Patients treated in our hosPital from January 2009 to June 2013 were obtained. The change of their blood glucose and correlated clinical data before and after chemotheraPy were retro-sPectively analyzed. Results:During chemotheraPy,15 cases develoPed secondary diabetes(15 / 215,6. 98% ),second-ary diabetes develoPed after chemotheraPy in 9 cases(9 / 215,4. 19% ). Their age were over 50 years old,19 cases aP-Peared glucosuria abnormal(19 / 215,8. 84% ),which aPPeared during chemotheraPy in 12 cases(12 / 215,5. 58% ),7 cases(7 / 215,3. 26% )after chemotheraPy,1 case of Poor blood sugar control for diabetes comPlications after chemo-theraPy dead,4. 17%(1 / 24),19 cases of Patients after diet control and Physical theraPy in 11 cases of fasting blood glucose levels back to normal(11 / 19,57. 89% ). Conclusion:For Patients with breast cancer treated with taxanes a-gent chemotheraPy can develoP secondary diabetes,there is certain correlation between them.%目的:观察紫杉类方案化疗对乳腺癌患者血糖的影响。方法:回顾性分析我院2009年1月-2013年6月收治的215例乳腺癌患者化疗前后的血糖变化情况及相关临床资料。结果:化疗期间出现继发性糖尿病15例(15/215,6.98%),化疗后出现继发性糖尿病9例(9/215,4.19%),其年龄均大于50岁;19例出现空腹血糖调节受损(19/215,8.84%),其中化疗期间出现12例(12/215,5.58%),化疗后出现7例(7/215,3.26%);1例继发性糖尿病患者血糖控制不好因酮症酸中毒而于化疗后10个月死亡(1/24,4.17%),19例空腹血糖调节受损患者中经过饮食控制及物理治疗11例空腹血糖水平恢复到正常水平(11/19,57.89%)。结论:乳腺癌患者应用紫杉类化疗方案化疗与继发性糖尿病或空腹血糖调节受损

  6. Shift measurements of the stark-broadened ionized helium lines at 1640 and 1215 angstrom. Ph.D. Thesis

    Science.gov (United States)

    Vanzandt, J. R.

    1976-01-01

    Time-resolved measurements were made of the shifts of the ionized helium lines at 1,640 A (n = 3 approaches 2) and 1,215 A (n = 4 approaches 2), and of the Stark profile of the 1,215 A wavelength line. An electromagnetic shock tube was used as a light source. The plasma conditions corresponded to electron temperatures of approximately 3.5 eV and electron densities of 0.8 to 1.8 x 10 to the 17th power/cubic cm. The measured shifts fell between two previous estimates of plasma polarization shifts. The measured Stark width of the 1,215 A wavelength line was up to 30% greater than the theoretical width.

  7. Early Paleogene variations in the calcite compensation depth: new constraints using old boreholes across Ninetyeast Ridge in the Indian Ocean

    Directory of Open Access Journals (Sweden)

    B. S. Slotnick

    2014-08-01

    Full Text Available Major variations in global carbon cycling occurred between 62 and 48 Ma. To better constrain the cause and magnitude of these changes, the community needs early Paleogene carbon isotope and carbonate accumulation records from widely separated deep-sea sediment sections, especially including the Indian Ocean. With the potential for renewed scientific drilling in the Indian Ocean, we examine lithologic, nannofossil assemblage, carbon isotope, and carbonate content records for late Paleocene – early Eocene sediment recovered at three existing sites spanning Ninetyeast Ridge: Deep Sea Drilling Project (DSDP Sites 213 (deep, east, 214 (shallow, central, and 215 (deep, west. The sediment sections are not ideal, because they were recovered in single holes using rotary coring methods. Site 214 was very shallow during the late Paleocene, when it received significant amounts of neritic carbonate. The δ13C records at Sites 213 and 215 are similar to those generated at several locations in the Atlantic and Pacific. The prominent high in δ13C across the Paleocene carbon isotope maximum (PCIM occurs at Site 215, and the prominent low in δ13C across the early Eocene Climatic Optimum (EECO occurs at both Site 213 and Site 215. The Paleocene–Eocene thermal maximum (PETM and the K/X event are found at Site 213 but not at Site 215, presumably because of coring gaps. Carbonate content at both Sites 213 and 215 drops to Discoaster lodoensis and the early Eocene rise in δ13C (~ 52 Ma. This reflects a rapid shoaling of the calcite compensation depth (CCD, and likely a major decrease in the net flux of 13C-depleted carbon to the ocean. Our work further constrains knowledge of the early Paleogene CCD, but more importantly suggests that excellent early Paleogene carbonate accumulation records might be recovered from the central Indian Ocean with future scientific drilling.

  8. Lactobacillus formosensis sp. nov., a lactic acid bacterium isolated from fermented soybean meal.

    Science.gov (United States)

    Chang, Chi-huan; Chen, Yi-sheng; Lee, Tzu-tai; Chang, Yu-chung; Yu, Bi

    2015-01-01

    A Gram-reaction-positive, catalase-negative, facultatively anaerobic, rod-shaped lactic acid bacterium, designated strain S215(T), was isolated from fermented soybean meal. The organism produced d-lactic acid from glucose without gas formation. 16S rRNA gene sequencing results showed that strain S215(T) had 98.74-99.60 % sequence similarity to the type strains of three species of the genus Lactobacillus (Lactobacillus farciminis BCRC 14043(T), Lactobacillus futsaii BCRC 80278(T) and Lactobacillus crustorum JCM 15951(T)). A comparison of two housekeeping genes, rpoA and pheS, revealed that strain S215(T) was well separated from the reference strains of species of the genus Lactobacillus. DNA-DNA hybridization results indicated that strain S215(T) had DNA related to the three type strains of species of the genus Lactobacillus (33-66 % relatedness). The DNA G+C content of strain S215(T) was 36.2 mol%. The cell walls contained peptidoglycan of the d-meso-diaminopimelic acid type and the major fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 cyclo ω10c/C19 : 1ω6c. Phenotypic and genotypic features demonstrated that the isolate represents a novel species of the genus Lactobacillus, for which the name Lactobacillus formosensis sp. nov. is proposed. The type strain is S215(T) ( = NBRC 109509(T) = BCRC 80582(T)).

  9. Evaluación del efecto de cuatro polimorfismos en el gen del receptor adrenérgico β-2 en el parto pretérmino

    Directory of Open Access Journals (Sweden)

    Juan Carlos Ríos

    2009-11-01

    Full Text Available Objective: To determine whether single nucleotide polymorphisms found in the sequence of the β-2 (β2AR adrenergic receptor are associated with spontaneous preterm delivery. Method: A case-control study in which the allelic, genotype, and haplotype frequencies of four polymorphisms, -47 C/T, -20 C/T, Arg16Gly, and Gln27Glu of the β2AR gene, were determined and compared between 35 women with preterm delivery (cases and 105 women with term delivery (controls. The study was performed with patients of three hospitals in the city of Medellín, Colombia. The genomic DNA of peripheral blood was isolated, the segment of the gene was amplified by means of the chain reaction of polymerase (PCR, and the polymorphisms were determined by direct sequence method. Results: No significant differences for the allelic frequencies were found for polymorphic loci -47 C/T, -20 C/T, Arg16Gly, and Gln27Glu between the cases group (21.5%, 21.5%, 51.5% and 21.5%, respectively and controls (21.5%, 21.5%, 57% and 21.5%, respectively; p>0.05. The genotype frequencies were also similar between patients with preterm delivery and those with term delivery (p>0.05 for all the genotypes. Only three of the 16 possible haplotypes (CCGG, TTGC, and TTAC, from the combination between the different alleles, appeared in the participants of this study. The haplotype frequencies were not significantly different between the compared groups either (p>0.05. Conclusion: No association was observed between preterm delivery and any of the polymorphisms or its haplotypes. These results do not support a role of the polymorphisms of the β2AR in spontaneous preterm delivery for these patients.

  10. Study of the enhancement effect of cyclopentadecanolide on protein permeation through lipid membranes

    Science.gov (United States)

    Li, Zhengmao

    Intranasal drug delivery has been a topic of increasing interest for a decade as a convenient and reliable method for the systemic administration of drugs. The low bioavailability of simple formulation of protein drugs, such as insulin, can be greatly improved by using permeation enhancers. We studied the effect of cyclopentadecanolide (CPE-215RTM) as a permeation enhancer in protein release through lipid bilayer membranes. We successfully designed a novel in-vitro membrane permeability model using liposomes and performed a series of transmembrane protein release experiments. These were carried out under a wide range of conditions in the presence of different permeation enhancer combinations. The experimental results showed that CPE-215RTM is an effective membrane permeation enhancer for proteins and a phase transfer agent, for example, cyclodextrins, can further enhance the effect of CPE-215RTM. Besides the release experiments, studies on insulin solution properties (self-diffusion and self-association states), the interaction between insulin and liposome and the interaction between CPE-215RTM and liposomes were carried out. Based on the mechanistic study and release data, we hypothesized that CPE-215RTM can form transient "pores" in the lipid bilayer that dissolve when CPE-215RTM distributes homogeneously within the bilayer and restore the barrier function of the lipid bilayer. We performed several experiments that corroborate our hypothesis. A mathematical model was developed based on our hypothesized release mechanism. A semi-empirical nonlinear equation involving four parameters effectively fits the protein release profiles. The quality of the data fit with this model is good supporting evidence for the validity of our mechanistic model. Finally we used a neural network approach to correlate the different release condition parameters and the four semi-empirical fitting parameters based on our limited data sets. Reasonable neural networks were formed for the

  11. GETDB: 112090 [GETDB

    Lifescience Database Archive (English)

    Full Text Available 112090 Link to Original w[*]; P{GawB}NP0215 / TM6, P{UAS-lacZ.UW23-1}UW23-1 82C4 Li...nk to DGRC Genome Viewer: 112090 5-HT2 CG12587 1360{}1206 215 3 NP line. Received from the National Institut...lls, sg uniform epi, tr sg, gut, tr - semi lethal - - d1 - - - Show 112090 DGRC Number 1120...C4 Map Viewer Link to DGRC Genome Viewer: 112090 Related Genes 5-HT2 CG12587 1360{}1206 Original Number 215

  12. 40 CFR Appendix A to Subpart Wwww... - Test Method for Determining Vapor Suppressant Effectiveness

    Science.gov (United States)

    2010-07-01

    ... Plastic Composites Production Pt. 63, Subpt. WWWW, App. A Appendix A to Subpart WWWW of Part 63—Test...—Wire Handle Roller P/N 205-050-300 or Plastic Handle Roller P/N 215-050-300 or equivalent; ES... Instrument, 630 Merrick Road, P.O. Box 329, Lynbrook, NY 11563, 516-593-5600 12 Insulating Board...

  13. Coping with Work Stressors in Nursing. Effects of Adaptive versus Maladaptive Strategies.

    Science.gov (United States)

    Parasuraman, Saroj; Hansen, Donna

    1987-01-01

    The effect of coping behaviors on nurses' affective reactions to work-generated stressors was assessed, using data gathered from 215 nurses in a medical center hospital in the Midwest. Adaptive coping, reflecting problem-solving behaviors, was found to moderate the relationships of work overload and resource inadequacy with felt stress. (Author/CH)

  14. Full Scale Test on a 100km, 150kV AC Cable

    DEFF Research Database (Denmark)

    Faria da Silva, Filipe Farria; Wiechowski, W.; Bak, Claus Leth;

    2010-01-01

    This paper presents some of the results obtained from the electrical measurements on a 99.7 km, 150 kV three-phase AC cable, connecting 215 MW offshore wind farm Horns Rev 2, located in Denmark west coast, to Denmark's 400 kV transmission network. The measurements were performed at nominal voltage...

  15. Timelapse scanning reveals spatial variation in tomato (Solanum lycopersicum L.) root elongation rates during partial waterlogging

    DEFF Research Database (Denmark)

    Dresbøll, Dorte Bodin; Thorup-Kristensen, Kristian; McKenzie, Blair M.;

    2013-01-01

    by increasing elongation rates. Methods Tomato plants (Solanum lycopersicum L.) were grown in peat in root chambers (300 × 215 × 6 mm) with a transparent front. Root chambers were maintained in flatbed scanners tilted at 30° to vertical and scanned every 3 h before, during and after waterlogging the lower layer...

  16. 78 FR 987 - Hazardous Materials: Harmonization with International Standards (RRR)

    Science.gov (United States)

    2013-01-07

    ... Hazardous Articles (COSTHA) Dangerous Goods Advisory Council (DGAC) Dow Chemical Company (Dow) Fuel Cell... (IMDG) Code, the International Civil Aviation Organization's Technical Instructions for the Safe.... Background PHMSA published a notice of proposed rulemaking (NPRM) under Docket HM-215L (77 FR 49167,...

  17. Data of evolutionary structure change: 1JE6A-2HJKA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1JE6A-2HJKA 1JE6 2HJK A A MEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDR...CA 188 ARG CA 262 ARG CA 318 ALA CA 382 2HJK... A 2HJKA VRFDSDAA...line>LEU CA 215 2HJK A 2HJK... 388 LEU CA 398 GLN CA 301 2HJK

  18. Catalyzing Employee Change with Transformative Learning

    Science.gov (United States)

    Franz, Nancy

    2010-01-01

    Businesses, organizations, and government agencies have invested heavily in employee training. The American Society for Training and Development (ASTD) reports these costs as 2.15% of payroll (ASTD, 2008). A large amount of this investment is directed at improving employee knowledge and skills. Although most organizations are good at this, often a…

  19. 29 CFR 4.171 - “Bona fide” fringe benefits.

    Science.gov (United States)

    2010-07-01

    ... adopted by the contractor, or by contract as a result of collective bargaining, must be specified in... Government contract, by law, or by the nature of the work to wear such items. See also § 4.168. (f... § 778.215 of 29 CFR part 778 in order for any contributions to be excluded from computation of...

  20. 77 FR 30181 - Continuation of the National Emergency With Respect to the Stabilization of Iraq

    Science.gov (United States)

    2012-05-21

    ..., 2012. [FR Doc. 2012-12487 Filed 5-18-12; 2:15 pm] Billing code 3295-F2-P ... Respect to the Stabilization of Iraq #0; #0; #0; Presidential Documents #0; #0; #0;#0;Federal Register... of Iraq On May 22, 2003, by Executive Order 13303, the President declared a national...