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Sample records for actinium 214

  1. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-08-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac-Cl and Ac-OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1- ligands (3.2+/-1.1) than AmIII (0.8+/-0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour.

  2. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-01-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac–Cl and Ac–OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1– ligands (3.2±1.1) than AmIII (0.8±0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour. PMID:27531582

  3. Discovery of the actinium, thorium, protactinium, and uranium isotopes

    CERN Document Server

    Fry, C

    2012-01-01

    Currently, 31 actinium, 31 thorium, 28 protactinium, and 23 uranium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  4. Production of high-purity radium-223 from legacy actinium-beryllium neutron sources.

    Science.gov (United States)

    Soderquist, Chuck Z; McNamara, Bruce K; Fisher, Darrell R

    2012-07-01

    Radium-223 is a short-lived alpha-particle-emitting radionuclide with potential applications in cancer treatment. Research to develop new radiopharmaceuticals employing (223)Ra has been hindered by poor availability due to the small quantities of parent actinium-227 available world-wide. The purpose of this study was to develop innovative and cost-effective methods to obtain high-purity (223)Ra from (227)Ac. We obtained (227)Ac from two surplus actinium-beryllium neutron generators. We retrieved the actinium/beryllium buttons from the sources and dissolved them in a sulfuric-nitric acid solution. A crude actinium solid was recovered from the solution by coprecipitation with thorium fluoride, leaving beryllium in solution. The crude actinium was purified to provide about 40 milligrams of actinium nitrate using anion exchange in methanol-water-nitric acid solution. The purified actinium was then used to generate high-purity (223)Ra. We extracted (223)Ra using anion exchange in a methanol-water-nitric acid solution. After the radium was separated, actinium and thorium were then eluted from the column and dried for interim storage. This single-pass separation produces high purity, carrier-free (223)Ra product, and does not disturb the (227)Ac/(227)Th equilibrium. A high purity, carrier-free (227)Th was also obtained from the actinium using a similar anion exchange in nitric acid. These methods enable efficient production of (223)Ra for research and new alpha-emitter radiopharmaceutical development.

  5. Relativistic small-core pseudopotentials for actinium, thorium, and protactinium.

    Science.gov (United States)

    Weigand, Anna; Cao, Xiaoyan; Hangele, Tim; Dolg, Michael

    2014-04-03

    Small-core pseudopotentials for actinium, thorium, and protactinium have been energy-adjusted to multiconfiguration Dirac-Hartree-Fock reference data based on the Dirac-Coulomb-Breit Hamiltonian and the Fermi nucleus model. Corresponding optimized valence basis sets of polarized valence quadruple-ζ quality are presented. Atomic test calculations for the first four ionization potentials show satisfactory results at both the Hartree-Fock and the multireference averaged coupled-pair functional level. Highly correlated Fock-space coupled cluster calculations demonstrate that the new pseudopotentials yield ionization potentials, which are in excellent agreement with corresponding all-electron results and experimental data. The pseudopotentials and basis sets supplement a similar set previously published for uranium.

  6. Effects of spin-orbit coupling on actinium under pressure

    Energy Technology Data Exchange (ETDEWEB)

    Rubio-Ponce, A.; Rivera, J. [Departamento de Ciencias Basicas, Universidad Autonoma Metropolitana-Azcapotzalco, Mexico (Mexico); Olguin, D. [Departamento de Fi sica, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Mexico (Mexico)

    2015-04-15

    Actinium (Ac) is a radioactive metal and the first element of the actinide series. At ambient conditions Ac crystallizes in the fcc lattice, however, up to date its phase diagram is unknown. In the present work, we have studied the structural and electronic properties of Ac under hydrostatic pressure assuming the fcc structure as well as three hypothetical structures, namely the hcp, bcc, and sc, and for pressures up to 100 GPa. From our calculations, we found only one structural transition allowed, from the fcc to hcp, our calculated pressure was 39.85 GPa. The calculations were performed by means of the full potential linearized augmented plane wave (FLAPW) method and the generalized gradient approximation (GGA) for the exchange-correlation energy, where we have included in our study the spin-orbit coupling which is important for heavy elements. The total energy results were fitted to the third order Birch-Murnaghan's equation of state. (copyright 2015 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  7. Production of Actinium-225 via High Energy Proton Induced Spallation of Thorium-232

    Energy Technology Data Exchange (ETDEWEB)

    Harvey, James T.; Nolen, Jerry; Vandergrift, George; Gomes, Itacil; Kroc, Tom; Horwitz, Phil; McAlister, Dan; Bowers, Del; Sullivan, Vivian; Greene, John

    2011-12-30

    The science of cancer research is currently expanding its use of alpha particle emitting radioisotopes. Coupled with the discovery and proliferation of molecular species that seek out and attach to tumors, new therapy and diagnostics are being developed to enhance the treatment of cancer and other diseases. This latest technology is commonly referred to as Alpha Immunotherapy (AIT). Actinium-225/Bismuth-213 is a parent/daughter alpha-emitting radioisotope pair that is highly sought after because of the potential for treating numerous diseases and its ability to be chemically compatible with many known and widely used carrier molecules (such as monoclonal antibodies and proteins/peptides). Unfortunately, the worldwide supply of actinium-225 is limited to about 1,000mCi annually and most of that is currently spoken for, thus limiting the ability of this radioisotope pair to enter into research and subsequently clinical trials. The route proposed herein utilizes high energy protons to produce actinium-225 via spallation of a thorium-232 target. As part of previous R and D efforts carried out at Argonne National Laboratory recently in support of the proposed US FRIB facility, it was shown that a very effective production mechanism for actinium-225 is spallation of thorium-232 by high energy proton beams. The base-line simulation for the production rate of actinium-225 by this reaction mechanism is 8E12 atoms per second at 200 MeV proton beam energy with 50 g/cm2 thorium target and 100 kW beam power. An irradiation of one actinium-225 half-life (10 days) produces {approx}100 Ci of actinium-225. For a given beam current the reaction cross section increases slightly with energy to about 400 MeV and then decreases slightly for beam energies in the several GeV regime. The object of this effort is to refine the simulations at proton beam energies of 400 MeV and above up to about 8 GeV. Once completed, the simulations will be experimentally verified using 400 MeV and 8 Ge

  8. Report for General Research September 18 to December 11, 1950 (Actinium Volume)

    Energy Technology Data Exchange (ETDEWEB)

    Haring, M.M.

    1951-01-15

    The purpose of the research work presented in this volume is to develop a process for the separation and purification of actinium-227 produced by neutron bombardment of radium-226 and to develop methods by which uniform films of actinium metal may be deposited on metallic surfaces. The design work on the cave structure and mechanical equipment used in the actinium separation is proceeding on schedule. As the mechanical design phase is nearing completion the emphasis is being directed toward processing equipment. The process as well as the mechanical equipment has been adapted from the research work of F. T. Hagemann and the Remote Control Group at Argonne National Laboratory. Consequently, one of the first objectives is to become familiary with the chemistry of the process and the operation of the mechanical equipment. Cold runs have been made on the T.T.A. benzene extraction using lanthanum and barium in place of actinium and radium. No difficulty with the operation was observed. The formation of precipitates was one of the difficulties encountered with the process as the precipitates carry radium. It has been found that metals such as nickel cause these precipitates to form and should, therefore, be avoided in the construction of equipment. it was also found that a T.T.A. solution exposed to 0.5 curie of polonium over a period of days develops a precipitate. Some new mechanical features hav eshown promise. The use of copper-coated glassware which will hold together even though the glass is cracked has made it possible to replace custom-built heaters with standard heating mantles. A new graphite, silicone grease mixture appears to hold up in stopcocks handling benzene and, as a result, may eliminate the necessary of entering the cave for regreasing. Tests on the preparation of dense concrete have given results which meet the shielding requirements for the cave. A strippable paint and tape combination has been studied and specified to provide for decontamination of

  9. Analysis of the gamma spectra of the uranium, actinium, and thorium decay series

    Energy Technology Data Exchange (ETDEWEB)

    Momeni, M.H.

    1981-09-01

    This report describes the identification of radionuclides in the uranium, actinium, and thorium series by analysis of gamma spectra in the energy range of 40 to 1400 keV. Energies and absolute efficiencies for each gamma line were measured by means of a high-resolution germanium detector and compared with those in the literature. A gamma spectroscopy method, which utilizes an on-line computer for deconvolution of spectra, search and identification of each line, and estimation of activity for each radionuclide, was used to analyze soil and uranium tailings, and ore.

  10. Application of ion exchange and extraction chromatography to the separation of actinium from proton-irradiated thorium metal for analytical purposes.

    Science.gov (United States)

    Radchenko, V; Engle, J W; Wilson, J J; Maassen, J R; Nortier, F M; Taylor, W A; Birnbaum, E R; Hudston, L A; John, K D; Fassbender, M E

    2015-02-06

    Actinium-225 (t1/2=9.92d) is an α-emitting radionuclide with nuclear properties well-suited for use in targeted alpha therapy (TAT), a powerful treatment method for malignant tumors. Actinium-225 can also be utilized as a generator for (213)Bi (t1/2 45.6 min), which is another valuable candidate for TAT. Actinium-225 can be produced via proton irradiation of thorium metal; however, long-lived (227)Ac (t1/2=21.8a, 99% β(-), 1% α) is co-produced during this process and will impact the quality of the final product. Thus, accurate assays are needed to determine the (225)Ac/(227)Ac ratio, which is dependent on beam energy, irradiation time and target design. Accurate actinium assays, in turn, require efficient separation of actinium isotopes from both the Th matrix and highly radioactive activation by-products, especially radiolanthanides formed from proton-induced fission. In this study, we introduce a novel, selective chromatographic technique for the recovery and purification of actinium isotopes from irradiated Th matrices. A two-step sequence of cation exchange and extraction chromatography was implemented. Radiolanthanides were quantitatively removed from Ac, and no non-Ac radionuclidic impurities were detected in the final Ac fraction. An (225)Ac spike added prior to separation was recovered at ≥ 98%, and Ac decontamination from Th was found to be ≥ 10(6). The purified actinium fraction allowed for highly accurate (227)Ac determination at analytical scales, i.e., at (227)Ac activities of 1-100 kBq (27 nCi to 2.7 μCi). Copyright © 2014 Elsevier B.V. All rights reserved.

  11. 23 CFR 646.214 - Design.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Design. 646.214 Section 646.214 Highways FEDERAL HIGHWAY... Projects § 646.214 Design. (a) General. (1) Facilities that are the responsibility of the railroad for maintenance and operation shall conform to the specifications and design standards used by the railroad in...

  12. 24 CFR 884.214 - Marketing.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Marketing. 884.214 Section 884.214... HOUSING PROJECTS Project Development and Operation § 884.214 Marketing. (a) Compliance with equal opportunity requirements. Marketing of units and selection of Families by the Owner shall be in accordance...

  13. 24 CFR 214.500 - Audit.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Audit. 214.500 Section 214.500... PROGRAM Other Federal Requirements § 214.500 Audit. Housing counseling grant recipients and subrecipients shall be subject to the audit requirements contained in 24 CFR parts 84 and 85. HUD must be provided...

  14. 5 CFR 551.214 - Statutory exclusion.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Statutory exclusion. 551.214 Section 551.214 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION UNDER THE FAIR LABOR STANDARDS ACT Exemptions and Exclusions § 551.214 Statutory exclusion....

  15. 24 CFR 214.311 - Funding.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Funding. 214.311 Section 214.311... PROGRAM Program Administration § 214.311 Funding. (a) HUD funding. HUD approval or program participation does not guarantee funding from HUD. Funding for the Housing Counseling program depends...

  16. 49 CFR 214.323 - Foul time.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Foul time. 214.323 Section 214.323 Transportation... TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.323 Foul time. Working limits established on controlled track through the use of foul time procedures shall comply with the...

  17. 25 CFR 214.10 - Royalty rates.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Royalty rates. 214.10 Section 214.10 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.10 Royalty rates. Royalties will be required as follows, subject to the approval of the President,...

  18. 49 CFR 214.325 - Train coordination.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Train coordination. 214.325 Section 214.325..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.325 Train coordination. Working limits established by a roadway worker through the use of train coordination shall comply with the...

  19. 49 CFR 214.305 - Compliance dates.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Compliance dates. 214.305 Section 214.305..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.305 Compliance dates. Each program adopted by a railroad shall comply not later than the date specified in the...

  20. 10 CFR 1021.214 - Adjudicatory proceedings.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Adjudicatory proceedings. 1021.214 Section 1021.214 Energy... Decisionmaking § 1021.214 Adjudicatory proceedings. (a) This section applies to DOE proposed actions that involve DOE adjudicatory proceedings, excluding judicial or administrative civil or criminal...

  1. Thorium and actinium polyphosphonate compounds as bone-seeking alpha particle-emitting agents.

    Science.gov (United States)

    Henriksen, Gjermund; Bruland, Oyvind S; Larsen, Roy H

    2004-01-01

    The present study explores the use of alpha-particle-emitting, bone-seeking agents as candidates for targeted radiotherapy. Actinium and thorium 1,4,7,10 tetraazacyclododecane N,N',N'',N''' 1,4,7,10-tetra(methylene) phosphonic acid (DOTMP) and thorium-diethylene triamine N,N',N'' penta(methylene) phosphonic acid (DTMP) were prepared and their biodistribution evaluated in conventional Balb/C mice at four hours after injection. All three bone-seeking agents showed a high uptake in bone and a low uptake in soft tissues. Among the soft tissue organs, only kidney had a relatively high uptake. The femur/kidney ratios for 227Th-DTMP, 228-Ac-DOTMP and 227Th-DOTMP were 14.2, 7.6 and 6.0, respectively. A higher liver uptake of 228Ac-DOTMP was seen than for 227Th-DTMP and 227Th-DOTMP. This suggests that some demetallation of the 228Ac-DOTMP complex had occurred. The results indicate that 225Ac-DOTMP, 227Th-DOTMP and 227Th-DTMP have promising properties as potential therapeutic bone-seeking agents.

  2. Developments towards in-gas-jet laser spectroscopy studies of actinium isotopes at LISOL

    Energy Technology Data Exchange (ETDEWEB)

    Raeder, S., E-mail: s.raeder@gsi.de [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Helmholtz-Institut Mainz, 55128 Mainz (Germany); GSI Helmholtzzentrum für Schwerionenforschung GmbH, Planckstraße 1, 64291 Darmstadt (Germany); Bastin, B. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); Block, M. [Helmholtz-Institut Mainz, 55128 Mainz (Germany); GSI Helmholtzzentrum für Schwerionenforschung GmbH, Planckstraße 1, 64291 Darmstadt (Germany); Institut für Kernchemie, Johannes Gutenberg Universität, 55128 Mainz (Germany); Creemers, P. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Delahaye, P. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); Ferrer, R. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Fléchard, X. [LPC Caen, ENSICAEN, Université de Caen, CNRS/IN2P3, Caen (France); Franchoo, S. [Institute de Physique Nucléaire (IPN) d’Orsay, 91406 Orsay, Cedex (France); Ghys, L. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); SCK-CEN, Belgian Nuclear Research Center, Boeretang 200, 2400 Mol (Belgium); Gaffney, L.P.; Granados, C. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Heinke, R. [Institut für Physik, Johannes Gutenberg Universität, 55128 Mainz (Germany); Hijazi, L. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); and others

    2016-06-01

    To study exotic nuclides at the borders of stability with laser ionization and spectroscopy techniques, highest efficiencies in combination with a high spectral resolution are required. These usually opposing requirements are reconciled by applying the in-gas-laser ionization and spectroscopy (IGLIS) technique in the supersonic gas jet produced by a de Laval nozzle installed at the exit of the stopping gas cell. Carrying out laser ionization in the low-temperature and low density supersonic gas jet eliminates pressure broadening, which will significantly improve the spectral resolution. This article presents the required modifications at the Leuven Isotope Separator On-Line (LISOL) facility that are needed for the first on-line studies of in-gas-jet laser spectroscopy. Different geometries for the gas outlet and extraction ion guides have been tested for their performance regarding the acceptance of laser ionized species as well as for their differential pumping capacities. The specifications and performance of the temporarily installed high repetition rate laser system, including a narrow bandwidth injection-locked Ti:sapphire laser, are discussed and first preliminary results on neutron-deficient actinium isotopes are presented indicating the high capability of this novel technique.

  3. 24 CFR 214.315 - Recordkeeping.

    Science.gov (United States)

    2010-04-01

    ... participating housing counseling agency must maintain a recordkeeping system. The system must permit HUD to... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Recordkeeping. 214.315 Section 214.315 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued...

  4. 25 CFR 214.4 - Bonds.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Bonds. 214.4 Section 214.4 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF OSAGE RESERVATION LANDS, OKLAHOMA, FOR... reserved to change the amount of the bond in any particular case, or to require a new bond in...

  5. 42 CFR 93.214 - Institutional member.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Institutional member. 93.214 Section 93.214 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES HEALTH ASSESSMENTS AND HEALTH EFFECTS STUDIES OF HAZARDOUS SUBSTANCES RELEASES AND FACILITIES PUBLIC HEALTH SERVICE POLICIES ON...

  6. 24 CFR 1710.214 - Recreational facilities.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 5 2010-04-01 2010-04-01 false Recreational facilities. 1710.214... § 1710.214 Recreational facilities. (a) Submit a synopsis of the proposed plans and estimated cost of any proposed or partially constructed recreational facility disclosed in § 1710.114. This item should...

  7. 24 CFR 214.300 - Counseling services.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Counseling services. 214.300... HOUSING COUNSELING PROGRAM Program Administration § 214.300 Counseling services. (a) Basic requirements. (1) Agencies must provide counseling to current and potential homeowners and tenants to assist...

  8. 15 CFR 280.214 - Hearings.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Hearings. 280.214 Section 280.214 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade NATIONAL INSTITUTE OF... proceedings will be taken by reporter or by electronic recording, transcribed and filed with...

  9. 28 CFR 2.14 - Subsequent proceedings.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Subsequent proceedings. 2.14 Section 2.14... proceedings. (a) Interim proceedings. The purpose of an interim hearing required by 18 U.S.C. 4208(h) shall be... date or commence rescission proceedings as provided by § 2.34; (iv) Advance the parole date...

  10. 29 CFR 1603.214 - Evidence.

    Science.gov (United States)

    2010-07-01

    ... STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE GOVERNMENT EMPLOYEE RIGHTS ACT OF 1991 Hearings § 1603.214 Evidence. The administrative law judge...

  11. Dicty_cDB: VHI214 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VH (Link to library) VHI214 (Link to dictyBase) - G22359 DDB0204095 Contig-U12490-1 VHI2...14P (Link to Original site) VHI214F 660 VHI214Z 705 VHI214P 1345 - - Show VHI214 Library VH (Link to library) Clone ID VHI2...Contig-U12490-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/VH/VHI2-A/VHI2...14Q.Seq.d/ Representative seq. ID VHI214P (Link to Original site) Representative DNA sequence >VHI214 (VHI214Q) /CSM/VH/VHI2...-A/VHI214Q.Seq.d/ GAATTAGAATGTAGTGGTAATGTAAATGGAATATGTAATAACAATGGTACATGTACTTGT AAACCAC

  12. 24 CFR 214.103 - Approval criteria.

    Science.gov (United States)

    2010-04-01

    ... counseling staff must possess a working knowledge of HUD's housing and single-family mortgage insurance... COUNSELING PROGRAM Approval and Disapproval of Housing Counseling Agencies § 214.103 Approval criteria. The... counseling agencies, branches, and affiliates that are included in one application: (a) Nonprofit and...

  13. 32 CFR 552.214 - Procedures.

    Science.gov (United States)

    2010-07-01

    ... on the Installation of Aberdeen Proving Ground, Maryland § 552.214 Procedures. (a) Any person or... Ground must submit a written request to the Commander, U.S. Army Garrison, Aberdeen Proving Ground, ATTN: STEAP-CO, 2201 Aberdeen Boulevard, Aberdeen Proving Ground, Maryland 21005-5001. The request must...

  14. 4 CFR 21.4 - Protective orders.

    Science.gov (United States)

    2010-01-01

    ... competitive advantage from access to the protected information and that there will be no significant risk of inadvertent disclosure of protected information. Objections to an applicant's admission shall be raised within... ACCOUNTABILITY OFFICE GENERAL PROCEDURES BID PROTEST REGULATIONS § 21.4 Protective orders. (a) At the request of...

  15. 22 CFR 214.43 - Agency records.

    Science.gov (United States)

    2010-04-01

    ..., the A.I.D. Auditor General, the OMB Secretariat, and the Comptroller General shall have access to..., and termination of the committee; (3) Annual reports of committee activity; (4) Designations of... § 214.36(b) (i.e., the reports, transcripts, minutes, appendices, and other documents which were made...

  16. 29 CFR 553.214 - Trainees.

    Science.gov (United States)

    2010-07-01

    ..., constitutes engagement in activities under section 7(k) only when the employee meets all the applicable tests... FAIR LABOR STANDARDS ACT TO EMPLOYEES OF STATE AND LOCAL GOVERNMENTS Fire Protection and Law Enforcement Employees of Public Agencies Exemption Requirements § 553.214 Trainees. The attendance at a bona...

  17. Civil works 513 - 289 - 214: traffic disturbance

    CERN Multimedia

    Optical Fibre Section

    2005-01-01

    The TS/EL group informs you that, in order to create an optical fibre access for the GEANT2 project, the Rutherford road between buildings 289 and 214 will be closed to the public on the 13th of July 2005. Part of the Sophora parking will be closed during the works (week 28, 11th - 15th July). Please respect the road signs put into place. For more information, please contact 160484. Optical Fibre Section, TS/EL

  18. 30 CFR 77.214 - Refuse piles; general.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles; general. 77.214 Section 77.214... Installations § 77.214 Refuse piles; general. (a) Refuse piles constructed on or after July 1, 1971, shall be..., tipples, or other surface installations and such piles shall not be located over abandoned openings...

  19. 49 CFR 214.315 - Supervision and communication.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Supervision and communication. 214.315 Section 214... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.315 Supervision and communication. (a) When an employer assigns duties to a roadway worker that call for...

  20. 48 CFR 12.214 - Cost Accounting Standards.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Cost Accounting Standards. 12.214 Section 12.214 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Items 12.214 Cost Accounting Standards. Cost Accounting Standards (CAS) do not apply to contracts...

  1. 22 CFR 214.42 - Uniform pay guidelines.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Uniform pay guidelines. 214.42 Section 214.42... Advisory Committees § 214.42 Uniform pay guidelines. (a) A.I.D. follows OMB/CSC guidelines in section 11 of... experts, their compensation shall be fixed in accordance with CSC guidelines and regulations, and...

  2. 48 CFR 852.214-73 - Alternate packaging and packing.

    Science.gov (United States)

    2010-10-01

    ... packing. 852.214-73 Section 852.214-73 Federal Acquisition Regulations System DEPARTMENT OF VETERANS....214-73 Alternate packaging and packing. As prescribed in 814.201-6(b)(3), insert the following provision: Alternate Packaging and Packing (JAN 2008) The bidder's offer must clearly indicate the...

  3. 25 CFR 214.25 - Forfeiture of lease.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Forfeiture of lease. 214.25 Section 214.25 Indians BUREAU..., OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.25 Forfeiture of lease. On the failure of any lessee or assignee to comply with any regulation or any obligation in the lease or assignment, the Secretary of...

  4. 36 CFR 2.14 - Sanitation and refuse.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Sanitation and refuse. 2.14 Section 2.14 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR RESOURCE PROTECTION, PUBLIC USE AND RECREATION § 2.14 Sanitation and refuse. (a) The following...

  5. 40 CFR 86.214-94 - Analytical gases.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false Analytical gases. 86.214-94 Section 86.214-94 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED... Passenger Vehicles; Cold Temperature Test Procedures § 86.214-94 Analytical gases. The provisions of §...

  6. 38 CFR 3.214 - Court decisions; unremarried surviving spouses.

    Science.gov (United States)

    2010-07-01

    ...; unremarried surviving spouses. 3.214 Section 3.214 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF... Requirements § 3.214 Court decisions; unremarried surviving spouses. Effective July 15, 1958, a decision rendered by a Federal court in an action to which the United States was a party holding that a...

  7. 29 CFR 825.214 - Employee right to reinstatement.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Employee right to reinstatement. 825.214 Section 825.214... THE FAMILY AND MEDICAL LEAVE ACT OF 1993 Employee Leave Entitlements Under the Family and Medical Leave Act § 825.214 Employee right to reinstatement. General rule. On return from FMLA leave,...

  8. 48 CFR 214.202-5 - Descriptive literature.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Descriptive literature. 214.202-5 Section 214.202-5 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... 214.202-5 Descriptive literature. (c) Requirements of invitation for bids. When brand name or...

  9. 29 CFR 1910.214 - Cooperage machinery. [Reserved

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 5 2010-07-01 2010-07-01 false Cooperage machinery. 1910.214 Section 1910.214 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.214 Cooperage machinery....

  10. 5 CFR 179.214 - Interest, penalties and administrative costs.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Interest, penalties and administrative costs. 179.214 Section 179.214 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS CLAIMS COLLECTION STANDARDS Salary Offset § 179.214 Interest, penalties and administrative...

  11. Dicty_cDB: VSI214 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VS (Link to library) VSI214 (Link to dictyBase) - - - Contig-U09738-1 VSI214Z (Link to Original si...ycdb.biol.tsukuba.ac.jp/CSM/VS/VSI2-A/VSI214Q.Seq.d/ Representative seq. ID VSI214Z (Link to Original si...nxppliqlvk kklknipkxvlerekk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value VSI...te) - - VSI214Z 582 - - - - Show VSI214 Library VS (Link to library) Clone ID VSI214 (Link to... dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig Contig-U09738-1 Original site URL http://dict

  12. 32 CFR Appendix A to Part 45 - DD Form 214

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 214 A Appendix A to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. A Appendix A to Part 45—DD Form 214 EC23OC91.003 EC23OC91.004 EC23OC91.005 EC23OC91.006...

  13. 20 CFR 408.214 - Are you age 65?

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Are you age 65? 408.214 Section 408.214... Qualification and Entitlement Age § 408.214 Are you age 65? You become age 65 on the first moment of the day before the anniversary of your birth corresponding to age 65. Thus, you must have been born on or...

  14. 5 CFR 531.214 - Setting pay upon promotion.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Setting pay upon promotion. 531.214... Changes § 531.214 Setting pay upon promotion. (a) General. An agency must set an employee's payable rate of basic pay upon promotion following the rules in this section, consistent with 5 U.S.C....

  15. 27 CFR 24.214 - Spanish type blending sherry.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Spanish type blending sherry. 24.214 Section 24.214 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... tax, not for sale or consumption as beverage wine. Wine of a high solids content and dark in...

  16. 22 CFR 214.11 - Establishment and chartering requirements.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Establishment and chartering requirements. 214... MANAGEMENT Establishment of Advisory Committees § 214.11 Establishment and chartering requirements. Provisions governing the establishment and chartering of Advisory Committees are contained in section 9 of...

  17. 7 CFR 1280.214 - Refund escrow accounts.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Refund escrow accounts. 1280.214 Section 1280.214 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... is a member of the Federal Reserve System and will deposit into such account an amount equal to...

  18. 12 CFR 214.5 - Accounts with foreign banks.

    Science.gov (United States)

    2010-01-01

    ... RELATIONS WITH FOREIGN BANKS AND BANKERS (REGULATION N) Regulations § 214.5 Accounts with foreign banks. (a) Any Federal Reserve Bank, with the consent of the Board, may open and maintain accounts payable in... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Accounts with foreign banks. 214.5 Section...

  19. 31 CFR 800.214 - Foreign government-controlled transaction.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Foreign government-controlled transaction. 800.214 Section 800.214 Money and Finance: Treasury Regulations Relating to Money and Finance... control of a U.S. business by a foreign government or a person controlled by or acting on behalf of...

  20. 48 CFR 633.214-70 - Alternative dispute resolution.

    Science.gov (United States)

    2010-10-01

    ... resolution. 633.214-70 Section 633.214-70 Federal Acquisition Regulations System DEPARTMENT OF STATE GENERAL... resolution. (a) Policy. The Department's goal is to resolve contract disputes before the issuance of a... resolution will have a significant impact on other pending cases or on the future conduct of...

  1. 24 CFR 214.313 - Housing counseling fees.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Housing counseling fees. 214.313... HOUSING COUNSELING PROGRAM Program Administration § 214.313 Housing counseling fees. (a) Participating agencies may charge reasonable and customary fees for housing education and counseling services, as long...

  2. 5 CFR 214.402 - Career reserved positions.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Career reserved positions. 214.402... EXECUTIVE SERVICE Types of Positions § 214.402 Career reserved positions. (a) The head of each agency is responsible for designating career reserved positions in accordance with the regulations in this section....

  3. 40 CFR 262.214 - Laboratory management plan.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Laboratory management plan. 262.214... Waste Determination and Accumulation of Unwanted Material for Laboratories Owned by Eligible Academic Entities § 262.214 Laboratory management plan. An eligible academic entity must develop and retain...

  4. 48 CFR 1552.214-71 - Contract award-other factors-formal advertising.

    Science.gov (United States)

    2010-10-01

    ... factors-formal advertising. 1552.214-71 Section 1552.214-71 Federal Acquisition Regulations System... Provisions and Clauses 1552.214-71 Contract award—other factors—formal advertising. As prescribed in 1514.201... Advertising” (FAR 52.214-10), and “Contract Award—Construction” (FAR 52.214-19). All other...

  5. 50 CFR 226.214 - Critical habitat for Gulf sturgeon.

    Science.gov (United States)

    2010-10-01

    ... ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS DESIGNATED CRITICAL HABITAT § 226.214... habitat units are depicted for Louisiana, Mississippi, Alabama and Florida on the maps below. The textual... Counties, Alabama. (1) Unit 3 includes the Conecuh River main stem beginning just downstream of...

  6. 9 CFR 113.214 - Parvovirus Vaccine, Killed Virus (Canine).

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Parvovirus Vaccine, Killed Virus... REQUIREMENTS Killed Virus Vaccines § 113.214 Parvovirus Vaccine, Killed Virus (Canine). Parvovirus Vaccine... established as follows: (1) Twenty-five parvovirus susceptible dogs (20 vaccinates and 5 controls) shall be...

  7. 7 CFR 1160.214 - Charges and penalties.

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Milk), DEPARTMENT OF AGRICULTURE FLUID MILK PROMOTION PROGRAM Fluid Milk Promotion Order National Fluid Milk Processor Promotion Board § 1160.214 Charges and penalties. (a)...

  8. 18 CFR 157.214 - Increase in storage capacity.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Increase in storage... Act for Certain Transactions and Abandonment § 157.214 Increase in storage capacity. (a) Prior notice. Subject to the notice requirements of § 157.205, the certificate holder is authorized to increase the...

  9. 48 CFR 2052.214-72 - Bid evaluation.

    Science.gov (United States)

    2010-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2052.214-72 Bid evaluation. As... be made to that responsive, responsible bidder within the meaning of FAR Subpart 9.1 whose total bid... the bidder is responsible within the meaning of FAR 9.1, and whether the bidder...

  10. Phenotype abnormality: 214 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available 214 http://metadb.riken.jp/db/SciNetS_ria224i/cria224u1ria224u720i decreased sensitivity... under influence of abscisic acid ... decreased sensitivity toward ... 2-cis-abscisic acid ...

  11. 20 CFR 725.214 - Determination of relationship; surviving spouse.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Determination of relationship; surviving... Benefits) § 725.214 Determination of relationship; surviving spouse. An individual shall be considered to be the surviving spouse of a miner if: (a) The courts of the State in which the miner was...

  12. 34 CFR 685.214 - Closed school discharge.

    Science.gov (United States)

    2010-07-01

    ... EDUCATION, DEPARTMENT OF EDUCATION WILLIAM D. FORD FEDERAL DIRECT LOAN PROGRAM Borrower Provisions § 685.214... the Act. (4) The Secretary reports the discharge of a loan under this section to all credit reporting agencies to which the Secretary previously reported the status of the loan. (c) Borrower qualification...

  13. Urate-2-14C transport in the rat nephron

    Science.gov (United States)

    Kramp, Ronald A.; Lassiter, William E.; Gottschalk, Carl W.

    1971-01-01

    Intrarenal transport of urate-2-14C was studied in anesthetized rats using the microinjection technic. During saline diuresis, small volumes of urate-2-14C (0.24-0.48 mM) and inulin-3H were injected into surface proximal and distal convoluted tubules, and ureteral urine was collected serially. Total (74-96%) and direct (57-84%) urate recovery increased significantly the more distal the puncture site. Delayed recovery (±20%) remained approximately the same regardless of localization of the microinjection. After proximal injections, total and direct recoveries of urate-2-14C were significantly higher in rats treated with probenecid, pyrazinoate, or PAH than during saline diuresis alone, while the excretion rates were comparable after distal injection. Delayed recovery was not altered by drug administration. The decreased proximal reabsorption of urate is presumably due to an effect of the drugs on the luminal membrane of the nephron. For perfusion at high urate concentrations, nonradioactive urate was added to the injectate (0.89-1.78 mM). Urate-2-14C recovery was almost complete and there was no delayed excretion, demonstrating saturation kinetics. These findings are compatible with a carrier-mediated mechanism for urate transport probably located at the luminal border of the proximal tubular epithelium. No definitive evidence for urate secretion was found in these studies. PMID:5543881

  14. 48 CFR 52.214-21 - Descriptive Literature.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Descriptive Literature. 52....214-21 Descriptive Literature. As prescribed in 14.201-6(p)(1), insert the following provision: Descriptive Literature (APR 2002) (a) Descriptive literature, as used in this provision, means...

  15. 22 CFR 214.51 - Administrative review of denial for public access to records.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Administrative review of denial for public access to records. 214.51 Section 214.51 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Administrative Remedies § 214.51 Administrative review of denial for public access to...

  16. Investigation of Carbohydrate Compositions for Poplar Ⅰ-214 and Chinese Fir

    Institute of Scientific and Technical Information of China (English)

    HUANG Luohua; QIN Tefu; MAGARA Kengo

    2006-01-01

    The carbohydrate compositions of poplar Ⅰ-214 and Chinese fir were investigated by the methods of hydrolysis and HPLC.The result showed that the contents of glucose,xylose and arabinose in poplar Ⅰ-214 are higher than those in Chinese fir,while contents of rhamnose and mannose in poplar Ⅰ-214 are lower than those in Chinese fir.

  17. 12 CFR 741.214 - Report of crime or catastrophic act and Bank Secrecy Act compliance.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Report of crime or catastrophic act and Bank Secrecy Act compliance. 741.214 Section 741.214 Banks and Banking NATIONAL CREDIT UNION ADMINISTRATION... Unions § 741.214 Report of crime or catastrophic act and Bank Secrecy Act compliance. Any credit...

  18. 12 CFR 347.214 - Branch established under section 5 of the International Banking Act.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Branch established under section 5 of the International Banking Act. 347.214 Section 347.214 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY INTERNATIONAL BANKING Foreign Banks § 347.214 Branch established...

  19. 27 CFR 555.214 - Storage within types 1, 2, 3, and 4 magazines.

    Science.gov (United States)

    2010-04-01

    ..., 3, and 4 magazines. 555.214 Section 555.214 Alcohol, Tobacco Products, and Firearms BUREAU OF... Storage § 555.214 Storage within types 1, 2, 3, and 4 magazines. (a) Explosive materials within a magazine... materials are not to be unpacked or repacked inside a magazine or within 50 feet of a magazine, and must...

  20. 49 CFR 214.518 - Safe and secure positions for riders.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Safe and secure positions for riders. 214.518 Section 214.518 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... Hi-Rail Vehicles § 214.518 Safe and secure positions for riders. On or after March 1, 2004, a...

  1. 48 CFR 52.214-34 - Submission of Offers in the English Language.

    Science.gov (United States)

    2010-10-01

    ... the English Language. 52.214-34 Section 52.214-34 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.214-34 Submission of Offers in the English Language. As prescribed in 14.201-6(w), insert the following provision: Submission of Offers in the English Language (APR 1991) Offers submitted...

  2. 32 CFR Appendix B to Part 45 - DD Form 214ws

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 214ws B Appendix B to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. B Appendix B to Part 45—DD Form 214ws EC23OC91.007...

  3. 8 CFR 214.14 - Alien victims of certain qualifying criminal activity.

    Science.gov (United States)

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Alien victims of certain qualifying criminal activity. 214.14 Section 214.14 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS NONIMMIGRANT CLASSES § 214.14 Alien victims of certain qualifying criminal activity....

  4. 48 CFR 1852.214-70 - Caution to offerors furnishing descriptive literature.

    Science.gov (United States)

    2010-10-01

    ... furnishing descriptive literature. 1852.214-70 Section 1852.214-70 Federal Acquisition Regulations System... CLAUSES Texts of Provisions and Clauses 1852.214-70 Caution to offerors furnishing descriptive literature... Descriptive Literature (DEC 1988) Bidders are cautioned against furnishing as a part of their bids...

  5. MiR-214 inhibits cell growth in hepatocellular carcinoma through suppression of {beta}-catenin

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xiaojun [Liver Diseases Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou (China); Chen, Ji [Department of Gastrointestinal Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai (China); Li, Feng [Department of Pathology, Fujian Provincial Hospital, Fuzhou (China); Lin, Yanting [Liver Diseases Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou (China); Zhang, Xiaoping; Lv, Zhongwei [Department of Interventional Therapy, Shanghai 10th People' s Hospital, School of Medicine, Tongji University, Shanghai (China); Jiang, Jiaji, E-mail: jiang_jjcn@yahoo.com.cn [Liver Diseases Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou (China)

    2012-11-30

    Highlights: Black-Right-Pointing-Pointer miR-214 is frequently downregulated in human HCC cell lines and tissues. Black-Right-Pointing-Pointer miR-214 overexpression inhibits HCC cell growth in vitro and in vivo. Black-Right-Pointing-Pointer miR-214 directly targets {beta}-catenin 3 Prime -UTR in HCC cells. Black-Right-Pointing-Pointer miR-214 regulates {beta}-catenin downstream signaling molecules. -- Abstract: Mounting evidence has shown that microRNAs (miRNAs) are implicated in carcinogenesis and can function as oncogenes or tumor suppressor genes in human cancers. Recent profile studies of miRNA expression have documented a deregulation of miRNA (miR-214) in hepatocellular carcinoma (HCC). However, its potential functions and underlying mechanisms in hepatocarcinogenesis remain largely unknown. Here, we confirmed that miR-214 is significantly downregulated in HCC cells and specimens. Ectopic overexpression of miR-214 inhibited proliferation of HCC cells in vitro and tumorigenicity in vivo. Further studies revealed that miR-214 could directly target the 3 Prime -untranslated region (3 Prime -UTR) of {beta}-catenin mRNA and suppress its protein expression. Similar to the restoring miR-214 expression, {beta}-catenin downregulation inhibited cell growth, whereas restoring the {beta}-catenin expression abolished the function of miR-214. Moreover, miR-214-mediated reduction of {beta}-catenin resulted in suppression of several downstream genes including c-Myc, cyclinD1, TCF-1, and LEF-1. These findings indicate that miR-214 serves as tumor suppressor and plays substantial roles in inhibiting the tumorigenesis of HCC through suppression of {beta}-catenin. Given these, miR-214 may serve as a useful prognostic or therapeutic target for treatment of HCC.

  6. MicroRNA-214 Suppresses Gluconeogenesis by Targeting Activating Transcriptional Factor 4*

    Science.gov (United States)

    Li, Kai; Zhang, Jin; Yu, Junjie; Liu, Bin; Guo, Yajie; Deng, Jiali; Chen, Shanghai; Wang, Chunxia; Guo, Feifan

    2015-01-01

    Although the gluconeogenesis pathway is already a target for the treatment of type 2 diabetes, the potential role of microRNAs (miRNAs) in gluconeogenesis remains unclear. Here, we investigated the physiological functions of miR-214 in gluconeogenesis. The expression of miR-214 was suppressed by glucagon via protein kinase A signaling in primary hepatocytes, and miR-214 was down-regulated in the livers of fasted, high fat diet-induced diabetic and leptin receptor-mutated (db/db) mice. The overexpression of miR-214 in primary hepatocytes suppressed glucose production, and silencing miR-214 reversed this effect. Gluconeogenesis was suppressed in the livers of mice injected with an adenovirus expressing miR-214 (Ad-miR-214). Additionally, Ad-miR-214 alleviated high fat diet-induced elevation of gluconeogenesis and hyperglycemia. Furthermore, we found that activating transcription factor 4 (ATF4), a reported target of miR-214, can reverse the suppressive effect of miR-214 on gluconeogenesis in primary hepatocytes, and this suppressive effect was blocked in liver-specific ATF4 knock-out mice. ATF4 regulated gluconeogenesis via affecting forkhead box protein O1 (FOXO1) transcriptional activity. Finally, liver-specific miR-214 transgenic mice exhibited suppressed gluconeogenesis and reduced expression of ATF4, phosphoenolpyruvate carboxykinase, and glucose-6-phosphatase in liver. Taken together, our results suggest that the miR-214-ATF4 axis is a novel pathway for the regulation of hepatic gluconeogenesis. PMID:25657009

  7. Cardiac hypertrophy and dysfunction induced by overexpression of miR-214 in vivo.

    Science.gov (United States)

    Yang, Tao; Gu, Haihua; Chen, Xiaofan; Fu, Shaozhi; Wang, Cheng; Xu, Hongfei; Feng, Qiang; Ni, Yiming

    2014-12-01

    An increasing number of studies have demonstrated the critical role of microRNAs in the pathogenesis of cardiac hypertrophy and dysfunction. This study evaluated whether miR-214 plays a pivotal role in the development of cardiac hypertrophy and heart failure. In human tissues, miR-214 overexpression was determined to promote cardiac hypertrophy. We predicted miR-214 direct target by bioinformatics database and verifed it using luciferase dual reporting system. We silenced miR-214 using a specific antagomir in a pressure-overload mouse model of heart failure. Analysis of transgenic mice with cardiomyocyte-specific overexpression of miR-214 indicated that their hearts were 21% heavier than wild-type hearts and expressed several biochemical and functional markers consistent with dilated cardiomyopathy. These findings include enlarged left ventricular internal diameters, wall thinning, reduced ejection fraction, fractional shortening, and an increased fetal gene expression. The enhancer of zeste homolog 2 (EZH2) was confirmed as a direct target of miR-214 in cardiomyocytes. In vivo silencing of miR-214 using a specific antagomir rescued cardiac EZH2 expression and prevented cardiac hypertrophy and dysfunction. Taken together, these results suggest that miR-214 may induce pathologic cardiac hypertrophy in part by reducing EZH2 messenger RNA levels. MiR-214 may therefore be a potential therapeutic target for treating certain cardiac disease states. Copyright © 2014 Elsevier Inc. All rights reserved.

  8. Quantification of the {sup 214} Pb and {sup 214} Bi decay products before and after to reach the secular equilibria with the {sup 226} Ra; Cuantificacion de los productos de decaimiento {sup 214} Pb y {sup 214} Bi antes y despues de alcanzar el equilibrio secular con el {sup 226} Ra

    Energy Technology Data Exchange (ETDEWEB)

    Quintero P, E.; Rojas M, V.P.; Cervantes N, M.L.; Gaso P, M.I. [Gerencia de Seguridad Radiologica, ININ, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2002-07-01

    In this work a comparison between the {sup 226} Ra concentration and its decay products ({sup 214} Pb and {sup 214} Bi) in soil samples is presented before and after that the decay mentioned products reach the equilibria with the radium. Moreover, the obtained daughter/father ratio is presented; and the correction factor for the calculus of the {sup 214} Pb and {sup 214} Bi quantification without being necessary to wait until that the secular equilibria has been established. For the quantification of the concentration of the three radionuclides the gamma spectrometry technique was used. (Author)

  9. Downregulation of microRNA-214 and overexpression of FGFR-1 contribute to hepatocellular carcinoma metastasis.

    Science.gov (United States)

    Wang, Jian; Li, Jingwu; Wang, Xiuchao; Zheng, Chen; Ma, Weidong

    2013-09-13

    miR-214 is one of the most significantly downregulated microRNAs (miRNAs) in hepatocellular carcinoma (HCC). Fibroblast growth factor receptor 1 (FGFR-1) is a miR-214 target gene implicated in the progression of HCC. However, the roles of miR-214 and FGFR-1 in HCC are not fully understood. Here, we analyzed the expression of miR-214 and FGFR-1 in 65 cases of HCC and paired non-neoplastic tissue specimens using real-time PCR and Western blot (WB), respectively. Our data indicated that miR-214 was downregulated and FGFR-1 was overexpressed in HCC compared to the paired non-neoplastic tissues. The low miR-214 expression was correlated with portal vein invasion (p=0.016) and early recurrence (p=0.045) in HCC patients. Moreover, the low miR-214 expression was correlated with high positive rate of FGFR-1 in HCC cases (p=0.020). Our data further demonstrated that miR-214 overexpression in SK-HEP1 and HepG2 cells downregulated FGFR-1 expression and inhibited liver cancer cell invasion. The Luciferase assay results further demonstrated the targeted regulation of FGFR-1 by miR-214. In conclusion, our data indicate that the downregulation of miR-214 in HCC and the upregulation of its target gene FGFR-1 is associated with HCC progression. Therefore, miR-214 and FGFR-1 are potential prognostic markers and therapeutic targets in HCC.

  10. 43 CFR 2.14 - When can I get expedited processing?

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false When can I get expedited processing? 2.14 Section 2.14 Public Lands: Interior Office of the Secretary of the Interior RECORDS AND TESTIMONY; FREEDOM... and correct to the best of your knowledge and belief. For example, a requester within the category of...

  11. 7 CFR 1709.214 - Administrator's review and selection of grant awards.

    Science.gov (United States)

    2010-01-01

    ... pertinent information before making a decision about which, if any, applications to approve, the amount of.... 1709.214 Section 1709.214 Agriculture Regulations of the Department of Agriculture (Continued) RURAL... decision to make a grant award is at the discretion of the Administrator. The Administrator shall...

  12. 9 CFR 93.214 - Import permit and declaration for poultry.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Import permit and declaration for poultry. 93.214 Section 93.214 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS IMPORTATION OF CERTAIN ANIMALS, BIRDS, FISH,...

  13. 48 CFR 36.214 - Special procedures for price negotiation in construction contracting.

    Science.gov (United States)

    2010-10-01

    ... price negotiation in construction contracting. 36.214 Section 36.214 Federal Acquisition Regulations... negotiation in construction contracting. (a) Agencies shall follow the policies and procedures in part 15 when... scope of the work. If negotiations reveal errors in the Government estimate, the estimate shall...

  14. 42 CFR 431.214 - Notice in cases of probable fraud.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Notice in cases of probable fraud. 431.214 Section... Hearings for Applicants and Recipients Notice § 431.214 Notice in cases of probable fraud. The agency may... indicating that action should be taken because of probable fraud by the recipient; and (b) The facts have...

  15. 23 CFR 973.214 - Indian lands congestion management system (CMS).

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Indian lands congestion management system (CMS). 973.214... HIGHWAYS MANAGEMENT SYSTEMS PERTAINING TO THE BUREAU OF INDIAN AFFAIRS AND THE INDIAN RESERVATION ROADS PROGRAM Bureau of Indian Affairs Management Systems § 973.214 Indian lands congestion management...

  16. Regulation of mitochondrial morphology and cell cycle by microRNA-214 targeting Mitofusin2.

    Science.gov (United States)

    Bucha, Sudha; Mukhopadhyay, Debashis; Bhattacharyya, Nitai Pada

    2015-10-02

    Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by the increase in CAG repeats beyond 36 at the exon1 of the gene Huntingtin (HTT). Among the various dysfunctions of biological processes in HD, transcription deregulation due to abnormalities in actions of transcription factors has been considered to be one of the important pathological conditions. In addition, deregulation of microRNA (miRNA) expression has been described in HD. Earlier, expression of microRNA-214 (miR-214) has been shown to increase in HD cell models and target HTT gene; the expression of the later being inversely correlated to that of miR-214. In the present communication, we observed that the expressions of several HTT co-expressed genes are modulated by exogenous expression of miR-214 or by its mutant. Among several HTT co-expressed genes, MFN2 was shown to be the direct target of miR-214. Exogenous expression of miR-214, repressed the expression of MFN2, increased the distribution of fragmented mitochondria and altered the distribution of cells in different phases of cell cycle. In summary, we have shown that increased expression of miR-214 observed in HD cell model could target MFN2, altered mitochondrial morphology and deregulated cell cycle. Inhibition of miR-214 could be a possible target of intervention in HD pathogenesis.

  17. 46 CFR 199.214 - Immersion suits and thermal protective aids.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must... an immersion suit. (c) The immersion suits and thermal protective aids required under paragraphs...

  18. miR-214 promotes radioresistance in human ovarian cancer cells by targeting PETN.

    Science.gov (United States)

    Zhang, Qin; Zhang, Shuxiang

    2017-08-31

    Ovarian cancer is one of the leading causes of death among gynecological malignancies. Increasing evidence indicate that dysregulation of microRNAs (miRNAs) plays an important role in tumor radioresistance. The aim of the present study is to investigate whether microRNA-214 (miR-214) was involved in radioresistance of human ovarian cancer. Here, we showed that miR-214 was significantly up-regulated in ovarian cancer tissues and radioresistance ovarian cancer cell lines. Transfection of miR-214 agomir in radiosensitive ovarian cancer cell lines promoted them for resistance to ionizing radiation, whereas transfection of miR-214 antagomir in radioresistance ovarian cancer cell lines sensitized them to ionizing radiation again. Furthermore, we found miR-214 effectively promoted tumor radioresistance in xenograft animal experiment. Western blotting and quantitative real-time PCR demonstrated that miR-214 negatively regulated PTEN in radioresistance ovarian cancer cell lines and ovarian cancer tissues. Taken together, our data conclude that miR-214 contributes to radioresistance of ovarian cancer by directly targeting PTEN. © 2017 The Author(s).

  19. 25 CFR 214.21 - Inspection of lessees' books and records.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Inspection of lessees' books and records. 214.21 Section... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.21 Inspection of lessees' books... premises for the purpose of inspection, and their books and records showing manner of operations...

  20. 49 CFR 214.529 - In-service failure of primary braking system.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false In-service failure of primary braking system. 214... Maintenance Machines and Hi-Rail Vehicles § 214.529 In-service failure of primary braking system. (a) In the event of a total in-service failure of its primary braking system, an on-track roadway...

  1. MEF2C mediates the effect of microRNA-214 on inhibiting cardiomyocyte hypertrophy

    Institute of Scientific and Technical Information of China (English)

    TANG Chun-mei; ZHU Jie-ning; ZHU Wen-si; LIN Qiu-xiong; HU Zhi-qin; FU Yong-heng; ZHANG Meng-zhen; SHAN Zhi-xin

    2016-01-01

    AIM:To investigate the effect of miR-214 on cardiomyocyte hypertrophy and the expression of the potential target genes . METHODS:A cell model of hypertrophy was established based on angiotensin-Ⅱ( Ang-Ⅱ)-induced neonatal mouse ventricular car-diomyocytes (NMVCs).Dual luciferase reporter assay was performed to verify the interaction between miR-214 and the 3’ UTR of MEF2C.The expression of MEF2C and hypertrophy-related genes at mRNA and protein levels was determined by RT-qPCR and Wes-tern blotting, respectively.RESULTS:The expression of ANP, ACTA1,β-MHC and miR-214 was markedly increased in Ang-Ⅱ-in-duced hypertrophic cardiomyocytes .Dual luciferase reporter assay revealed that miR-214 interacted with the 3’ UTR of MEF2C, and miR-214 was verified to inhibit MEF2C expression at the transcriptional level .The protein expression of MEF2C was markedly in-creased in the hypertrophic cardiomyocytes .Moreover, miR-214 mimic, in parallel to MEF2C siRNA, inhibited the expression of hy-pertrophy-related genes in Ang-Ⅱ-induced NMVCs.CONCLUSION:MEF2C is a target gene of miR-214, which mediates the effect of miR-214 on attenuating cardiomyocyte hypertrophy .

  2. 29 CFR 2.14 - Proceedings in which the Department balances conflicting values.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Proceedings in which the Department balances conflicting values. 2.14 Section 2.14 Labor Office of the Secretary of Labor GENERAL REGULATIONS Audiovisual Coverage... know outbalances the individual's right to privacy. When audiovisual coverage is restricted or...

  3. Circulating miR-214 is associated with the severity of coronary artery disease

    Institute of Scientific and Technical Information of China (English)

    Hong-Quan Lu; Chun Liang; Zhi-Qing He; Min Fan; Zong-Gui Wu

    2013-01-01

    Objective To study whether miR-214 is regulated in coronary artery disease (CAD) patients and whether placental growth factor (PLGF) is a possible target for miR-214 in atherosclerosis. Methods Circulating miR-214 was measured by quantitative PCR using RNA isolated from 40 patients with CAD, including 12 with stable angina pectoris, 16 with unstable angina pectoris and 12 with acute myocardial infarction, and 15 controls without CAD. Plasma level of PLGF was measured by ELISA. Results The miR-214 level was significantly lower in CAD patients compared with that in controls (P < 0.01). Compared to controls, patients with unstable angina pectoris (UAP, 38.6± 9.1 pg/mL) and acute myocardial infarction (AMI, 46.3±13.4 pg/mL) had significantly higher level of plasma PLGF, but not those with stable angina pectoris (SAP; P = 0.012, UAP vs. Control; P = 0.005, AMI vs. Control). In patients with AMI, the plasma level of miR-214 was positively correlated to that of PLGF. Conclusions The results suggest that miR-214 is a beneficial microRNA for CAD patients. Loss of its protection may lead to increased PLGF levels and worsening atherosclerosis. Circulating miR-214 is a promising biomarker for alerting severe CAD.

  4. 76 FR 17473 - Twelfth Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

    Science.gov (United States)

    2011-03-29

    ... Federal Aviation Administration Twelfth Meeting: RTCA Special Committee 214: Working Group 78: Standards...: Notice of RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data Communication... Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services. DATES: The...

  5. 75 FR 20423 - Tenth Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

    Science.gov (United States)

    2010-04-19

    ... Federal Aviation Administration Tenth Meeting: RTCA Special Committee 214: Working Group 78: Standards for...: Notice of RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data Communication... Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services. DATES: The...

  6. 75 FR 66828 - Eleventh Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

    Science.gov (United States)

    2010-10-29

    ... Federal Aviation Administration Eleventh Meeting: RTCA Special Committee 214: Working Group 78: Standards...: Notice of RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data Communication... Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services. DATES: The...

  7. 76 FR 58078 - Thirteenth Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

    Science.gov (United States)

    2011-09-19

    ... Federal Aviation Administration Thirteenth Meeting: RTCA Special Committee 214: Working Group 78.... ACTION: Notice of RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data... Special Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services. DATES:...

  8. 76 FR 56362 - Removal of Approved Non-U.S.-Licensed Space Stations From the Section 214 Exclusion List

    Science.gov (United States)

    2011-09-13

    ... COMMISSION 47 CFR Part 63 Removal of Approved Non-U.S.-Licensed Space Stations From the Section 214 Exclusion... remove from the Section 214 Exclusion List those non-U.S.-licensed space stations that have been allowed... from the Section 214 Exclusion List those non-U.S.-licensed space stations that have been allowed...

  9. 49 CFR 214.521 - Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles.

    Science.gov (United States)

    2010-10-01

    ... maintenance machines and hi-rail vehicles. 214.521 Section 214.521 Transportation Other Regulations Relating... WORKPLACE SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.521 Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles. Each on-track roadway maintenance...

  10. 49 CFR 214.525 - Towing with on-track roadway maintenance machines or hi-rail vehicles.

    Science.gov (United States)

    2010-10-01

    ... or hi-rail vehicles. 214.525 Section 214.525 Transportation Other Regulations Relating to... SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.525 Towing with on-track roadway maintenance machines or hi-rail vehicles. (a) When used to tow pushcars or other maintenance-of-way...

  11. Oxidation behaviors of porous Haynes 214 alloy at high temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yan, E-mail: wangyan@csu.edu.cn [School of Aeronautics and Astronautics, Central South University, Changsha 410083 (China); Liu, Yong, E-mail: yonliu@csu.edu.cn [State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083 (China); Tang, Huiping, E-mail: hptang@c-nin.com [State Key Laboratory of Porous Metals Materials, Northwest Institute for Nonferrous Metal Research, Xi' an 710016 (China); Li, Weijie, E-mail: wl347@uowmail.edu.au [Institute for Superconducting and Electronic Materials, University of Wollongong, Wollongong, NSW 2522 (Australia)

    2015-09-15

    The oxidation behaviors of porous Haynes 214 alloy at temperatures from 850 to 1000 °C were investigated. The porous alloys before and after the oxidation were examined by optical microscopy, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) analyses, and X-ray photoelectron spectroscopy (XPS). The oxidation kinetics of the porous alloy approximately follows a parabolic rate law and exhibits two stages controlled by different oxidation courses. Complex oxide scales composed of Cr{sub 2}O{sub 3}, NiCr{sub 2}O{sub 4} and Al{sub 2}O{sub 3} are formed on the oxidized porous alloys, and the formation of Cr{sub 2}O{sub 3} on its outer layer is promoted with the oxidation proceeding. The rough surface as well as the micropores in the microstructures of the porous alloy caused by the manufacturing process provides fast diffusion paths for oxygen so as to affect the formation of the oxide layers. Both the maximum pore size and the permeability of the porous alloys decrease with the increase of oxidation temperature and exposure time, which may limit its applications. - Highlights: • Two-stage oxidation kinetics controlled by different oxidation courses is showed. • Oxide scale mainly consists of Cr{sub 2}O{sub 3}, NiCr{sub 2}O{sub 4} and Al{sub 2}O{sub 3}. • Rough surface and micropores lead to the formation of uneven oxide structure. • Content of Cr{sub 2}O{sub 3} in the outer layer of the scale increases with time at 1000 °C. • Maximum pore size and permeability decrease with increasing temperature and time.

  12. An analysis of 214 cases of rib fractures

    Directory of Open Access Journals (Sweden)

    Sule Karadayi

    2011-01-01

    Full Text Available INTRODUCTION: Rib fractures are the most common type of injury associated with trauma to the thorax. In this study, we investigated whether morbidity and mortality rates increased in correlation with the number of fractured ribs. MATERIALS AND METHODS: Data from 214 patients with rib fractures who applied or were referred to our clinic between January 2007 and December 2008 were retrospectively evaluated. The patients were allocated into three groups according to the number of fractures: 1 patients with an isolated rib fracture (RF1 (n = 50, 23.4%, 2 patients with two rib fractures (RF2 (n = 53, 24.8%, and 3 patients with more than two rib fractures (RF3 (n = 111, 51.9%. The patients were evaluated and compared according to the number of rib fractures, mean age, associated chest injuries (hemothorax, pneumothorax, and/or pulmonary contusion, and co-existing injuries to other systems. FINDINGS: The mean age of the patients was 51.5 years. The distribution of associated chest injuries was 30% in group RF1, 24.6% in group RF2, and 75.6% in group RF3 (p<0.05. Co-existing injuries to other systems were 24% in group RF1, 23.2% in group RF2, and 52.6% in group RF3 (p<0.05. Two patients (4% in group RF1, 2 patients (3.8% in group RF2, and 5 patients (4.5% in group RF3 (total n = 9; 4.2% died. CONCLUSION: Patients with any number of rib fractures should be carefully screened for co-existing injuries in other body systems and hospitalized to receive proper treatment.

  13. Cavitation erosion resistance of 13/4 and 21-4-N steels

    Indian Academy of Sciences (India)

    Akhilesh K Chauhan

    2013-02-01

    Nitrogen strengthened austenitic stainless steel (termed as 21-4-N steel) in as cast and hot rolled conditions has been investigated as an alternative to 13/4 steel (termed as CA6NM) to overcome the problems of cavitation erosion in hydro turbine underwater parts. The cavitation erosion of 21-4-N and 13/4 steels was investigated by means of an ultrasonic vibration processor. The cavitation erosion is highly dependent on microstructure and mechanical properties. The results show that hot rolled 21-4-N steel is more cavitation erosion resistant than the 13/4 and 21-4-N steels in as cast condition. The eroded surfaces were analysed through optical microscope and scanning electron microscope for study of erosion mechanisms.

  14. Lukas 2:14 vanuit ’n Joods-Christelike perspektief

    Directory of Open Access Journals (Sweden)

    A. P.B. Breytenbach

    1994-01-01

    Full Text Available Luke 2:14 from a Jewish Christian perspective The well-icnown Gloria in excelsis is a cryptic but thoroughly investigated text. This article critiques aspects of the prevailing exegesis of Luke 2:14. A ease is made out for reading the Gloria as a translated Hebrew text. The conventions of Hebrew poetry are taken into account and the obviously well-structured verse is interpreted against the background of information from Jewish and Jewish-Christian literature.

  15. Aluminium oxide nanoparticles induced morphological changes, cytotoxicity and oxidative stress in Chinook salmon (CHSE-214) cells.

    Science.gov (United States)

    Srikanth, Koigoora; Mahajan, Amit; Pereira, Eduarda; Duarte, Armando Costa; Venkateswara Rao, Janapala

    2015-10-01

    Aluminium oxide nanoparticles (Al2 O3 NPs) are increasingly used in diverse applications that has raised concern about their safety. Recent studies suggested that Al2 O3 NPs induced oxidative stress may be the cause of toxicity in algae, Ceriodaphnia dubia, Caenorhabditis elegans and Danio rerio. However, there is paucity on the toxicity of Al2 O3 NPs on fish cell lines. The current study was aimed to investigate Al2 O3 NPs induced cytotoxicity, oxidative stress and morphological abnormality of Chinnok salmon cells (CHSE-214). A dose-dependent decline in cell viability was observed in CHSE-214 cells exposed to Al2 O3 NPs. Oxidative stress induced by Al2 O3 NPs in CHSE-214 cells has resulted in the significant reduction of superoxide dismutase, catalase and glutathione in a dose-dependent manner. However, a significant increase in glutathione sulfo-transferase and lipid peroxidation was observed in CHSE-214 cells exposed to Al2 O3 NPs in a dose-dependent manner. Significant morphological changes in CHSE-214 cells were observed when exposed to Al2 O3 NPs at 6, 12 and 24 h. The cells started to detach and appear spherical at 6 h followed by loss of cellular contents resulting in the shrinking of the cells. At 24 h, the cells started to disintegrate and resulted in cell death. Our data demonstrate that Al2 O3 NPs induce cytotoxicity and oxidative stress in a dose-dependent manner in CHSE-214 cells. Thus, our current work may serve as a base-line study for future evaluation of toxicity studies using CHSE-214 cells.

  16. Corrective Action Plan for Corrective Action Unit 214: Bunkers and Storage Areas, Nevada Test Site, Nevada - Revision 0 - March 2005

    Energy Technology Data Exchange (ETDEWEB)

    U.S. Department of Energy, National Nuclear Security Administration, Nevada Site Office; Bechtel Nevada

    2005-03-01

    Corrective Action Unit 214, Bunkers and Storage Areas, is identified in the Federal Facility Agreement and Consent Order of 1996. Corrective Action Unit 214 consists of nine Corrective Action Sites located in Areas 5, 11, and 25 of the Nevada Test Site. The Nevada Test Site is located approximately 105 kilometers (65 miles) northwest of Las Vegas, Nevada, in Nye County. Corrective Action Unit 214 was previously characterized in 2004, and results were presented in the Corrective Action Decision Document for 214. Site characterization indicated that soil and/or debris exceeded clean-up criteria for Total Petroleum Hydrocarbons, pesticides, metals, and radiological contamination.

  17. MiR-214 regulates the function of osteoblast under simulated microgravity by targeting ATF4

    Science.gov (United States)

    Li, Yingxian; Wang, Xiaogang; Li, Qi; Lv, Ke; Wan, Yumin; Li, Yinghui; Bai, Yanqiang

    Background: MicroRNAs (miRNAs) are small fragments of single-stranded RNA containing 18-24 nucleotides, and are generated from endogenous transcripts. MicroRNAs function in post-transcriptional gene silencing by targeting the 3'-untranslated region (UTR) of mRNAs, resulting in translational repression. Growing evidence shows that microRNAs (miRNAs) regu-late various developmental and homeostatic events in vertebrates and invertebrates. Osteoblast differentiation is a key step in proper skeletal development and acquisition of bone mass; How-ever, the physiological role of non-coding small RNAs, especially miRNAs, in osteoblast dif-ferentiation remains elusive. Methods: To study the potential involvement of miRNAs in osteoblast differentiation under stimulated microgravity, we analyzed the expression of 20 bone relative miRNAs using real time PCR platform to find particularly miRNAs whose expression is altered during osteoblast differentiation. TargetScan, miRBase and Miranda were used to predict the target gene of candidate miRNA. To investigate whether ATF4 can be directly targeted by miR-214, we engineered luciferase reporters that have either the wild-type 3'UTRs of these genes, or the mutant UTRs with a 6 base pair (bp) deletion in the target sites. Lastly, to address the in vivo role of miR-214 in bone formation, tail suspension mice model was used to simulate the change of osteoblast function and bone loss. Results: Recent studies have sug-gested that miRNAs might play a role in osteoblast differentiation and bone formation. Here, we identify miR-214 in MC3T3-E1 cells, which is a primary mouse osteoblasts cell line, to promote osteoblast differentiation by repressing Activating Transcription Factor4 (ATF4) ex-pression at the posttranscriptional level. What is more, miR-214 was found to be transcribed in C2C12 cells during bone morphogenetic protein 2-induced (BMP2-induced) osteogenesis, and overexpression of miR-214 attenuated BMP2-induced osteoblastogenesis

  18. miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Zhengyu [Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai (China); Wang, Tao, E-mail: wangtaohappy2010@sohu.com [Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai (China)

    2014-06-27

    Highlights: • miR-214 is upregulated in human OS tissues and inversely correlated with LZTS1 expression. • miR-214 directly targets LZTS1 by binding to its 3′-UTR. • miR-214 promotes OS cell proliferation, invasion and tumor growth. • Overexpression of LZTS1 reverses miR-214-induced proliferation and invasion of OS cells. - Abstract: Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealed leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3′-untranslated region (3′-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.

  19. CT-guided percutaneous spine biopsy in suspected infection or malignancy. A study of 214 patients

    Energy Technology Data Exchange (ETDEWEB)

    Rehm, J.; Veith, S.; Kauczor, H.U.; Weber, M.A. [Heidelberg Univ. (Germany). Inst. of Diagnostic and Interventional Radiology; Akbar, M. [Heidelberg Univ. (Germany). Dept. of Orthopaedic Surgery and Rehabilitation Medicine

    2016-12-15

    To retrospectively determine the effectiveness and accuracy of CT-guided percutaneous biopsy of malignant and inflammatory bone lesions of the spine and to assess the reliability of pre-biopsy CT and MRI. 214 patients with lesions of the spine, which were suspicious either for being malignant or inflammatory, underwent CT-guided biopsy for pathological and/or microbiological detection. Biopsy samples were sent for histological examination in 128/214 patients, for microbiological analysis in 17/214 patients and for both analyses in 69/214 patients. Retrospectively, the diagnostic accuracy and sensitivity/specificity of the pre-interventional imaging (CT and MRI) were determined. In addition, the influence of the biopsy on subsequent patient management was assessed. The accuracy was 94.4% for histopathological analysis and 97.7% for microbiological analysis. In 25% of cases the microbiological analysis revealed an underlying pathogen that was not significantly affected by pre-biopsy antibiotic therapy. The sensitivity/specificity of the pre-biopsy cross-sectional imaging concerning suspected malignancy was 69%/78%. For suspected infection, the sensitivity/specificity of pre-biopsy imaging was 81%/44%. In 52% of all cases, the biopsy result changed subsequent patient management. Percutaneous CT-guided spine biopsy is a useful and reliable diagnostic procedure to establish a definitive diagnosis but with a relatively low yield of microorganisms in the case of infection.

  20. 10 CFR 72.214 - List of approved spent fuel storage casks.

    Science.gov (United States)

    2010-01-01

    ... 72.214 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C... Standardized NUHOMS® Horizontal Modular Storage System for Irradiated Nuclear Fuel. Docket Number:...

  1. 22 CFR 214.38 - Submission of reports to the Library of Congress.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Submission of reports to the Library of... MANAGEMENT Operation of Advisory Committees § 214.38 Submission of reports to the Library of Congress. (a) Each advisory committee is to file with the Library of Congress eight copies of each of its...

  2. 33 CFR 110.214 - Los Angeles and Long Beach harbors, California.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Los Angeles and Long Beach... HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.214 Los Angeles and Long Beach... the Captain of the Port Los Angeles-Long Beach, the pilot stations for the Port of Long Beach and...

  3. 33 CFR 157.214 - Required documents: U.S. tank vessels.

    Science.gov (United States)

    2010-07-01

    ... CARRYING OIL IN BULK Dedicated Clean Ballast Tanks on Tank Vessels General § 157.214 Required documents: U... ballast tank system plans; (b) The Coast Guard approved Dedicated Clean Ballast Tanks Operations Manual... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Required documents: U.S....

  4. 49 CFR Appendix A to Part 214 - Schedule of Civil Penalties 1

    Science.gov (United States)

    2010-10-01

    ... requirements for operating on-track roadway maintenance machine with non-complying headlights, work lights... 49 Transportation 4 2010-10-01 2010-10-01 false Schedule of Civil Penalties 1 A Appendix A to Part...—Schedule of Civil Penalties 1 Section Violation Willful Subpart B—Bridge Worker Safety Standards 214.103...

  5. 49 CFR 214.329 - Train approach warning provided by watchmen/lookouts.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Train approach warning provided by watchmen... Protection § 214.329 Train approach warning provided by watchmen/lookouts. Roadway workers in a roadway work... or more watchmen/lookouts in accordance with the following provisions: (a) Train approach...

  6. 49 CFR 214.349 - Training and qualification of watchmen/lookouts.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Training and qualification of watchmen/lookouts... Protection § 214.349 Training and qualification of watchmen/lookouts. (a) The training and qualification for roadway workers assigned the duties of watchmen/lookouts shall include, as a minimum, consideration of...

  7. 48 CFR 52.214-25 - Step Two of Two-Step Sealed Bidding.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Step Two of Two-Step... Clauses 52.214-25 Step Two of Two-Step Sealed Bidding. As prescribed in 14.201-6(t), insert the following provision: Step Two of Two-Step Sealed Bidding (APR 1985) (a) This invitation for bids is issued to...

  8. 49 CFR 214.335 - On-track safety procedures for roadway work groups.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false On-track safety procedures for roadway work groups... Protection § 214.335 On-track safety procedures for roadway work groups. (a) No employer subject to the provisions of this part shall require or permit a roadway worker who is a member of a roadway work group...

  9. 8 CFR 214.4 - Denial of certification, denial of recertification or withdrawal of SEVP certification.

    Science.gov (United States)

    2010-01-01

    ... HOMELAND SECURITY IMMIGRATION REGULATIONS NONIMMIGRANT CLASSES § 214.4 Denial of certification, denial of...(a)(15)(F)(i) and/or 101(a)(15)(M)(i) of the Immigration and Nationality Act, will be withdrawn on... consider the impact that such date will have upon SEVP, the school, and the school's nonimmigrant...

  10. 23 CFR 970.214 - Federal lands congestion management system (CMS).

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Federal lands congestion management system (CMS). 970....214 Federal lands congestion management system (CMS). (a) For purposes of this section, congestion...) Develop criteria to determine when a CMS is to be implemented for a specific transportation system; and...

  11. 23 CFR 972.214 - Federal lands congestion management system (CMS).

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Federal lands congestion management system (CMS). 972... § 972.214 Federal lands congestion management system (CMS). (a) For purposes of this section, congestion... interference. For those FWS transportation systems that require a CMS, in both metropolitan and...

  12. 25 CFR 166.214 - Will the BIA notify the permittee of any change in land title status?

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Will the BIA notify the permittee of any change in land title status? 166.214 Section 166.214 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND... permittee of any change in land title status? Yes. We will notify the permittee if a fee patent is issued...

  13. Radon and progeny ({sup 214}Pb and {sup 214}Bi) in urban water-supply systems of Sao Paulo State, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Bonotto, Daniel Marcos [Departamento de Petrologia e Metalogenia, Universidade Estadual Paulista (UNESP), Av. 24-A, No. 1515, C.P. 178, CEP 13506-900 Rio Claro, Sao Paulo (Brazil)], E-mail: danielbonotto@yahoo.com.br; Padron-Armada, Priscilla Cardoso [Departamento de Petrologia e Metalogenia, Universidade Estadual Paulista (UNESP), Av. 24-A, No. 1515, C.P. 178, CEP 13506-900 Rio Claro, Sao Paulo (Brazil)

    2008-10-15

    Many water-supply systems in South America utilize the waters of the Guarani aquifer at least as part of their networks. However, there is little present knowledge in Brazil of the factors affecting Rn presence in the water supplied for end-users, despite the economic importance of Guarani aquifer. {sup 222}Rn analyzes of 162 water samples were performed at 8 municipalities in Sao Paulo State, Brazil, with the aim of investigating the major factors affecting its presence in solution. The {sup 222}Rn activity concentration ranged from 0.04 up to 204.9 Bq/L, with three samples exceeding the World Health Organization maximum limit of 100 Bq/L. Aeration was confirmed as the most important factor for Rn release, as expected due to its gaseous nature. Accumulation in pipes and stratification in the water column were other significant factors explaining the data obtained in some circumstances. The Rn daughters {sup 214}Pb and {sup 214}Bi were also determined in a set of selected samples and their presence was directly related to the occurrence of Rn dissolved in water.

  14. The Oncogenic Fusion Proteins SET-Nup214 and Sequestosome-1 (SQSTM1)-Nup214 Form Dynamic Nuclear Bodies and Differentially Affect Nuclear Protein and Poly(A)+ RNA Export.

    Science.gov (United States)

    Port, Sarah A; Mendes, Adélia; Valkova, Christina; Spillner, Christiane; Fahrenkrog, Birthe; Kaether, Christoph; Kehlenbach, Ralph H

    2016-10-28

    Genetic rearrangements are a hallmark of several forms of leukemia and can lead to oncogenic fusion proteins. One example of an affected chromosomal region is the gene coding for Nup214, a nucleoporin that localizes to the cytoplasmic side of the nuclear pore complex (NPC). We investigated two such fusion proteins, SET-Nup214 and SQSTM1 (sequestosome)-Nup214, both containing C-terminal portions of Nup214. SET-Nup214 nuclear bodies containing the nuclear export receptor CRM1 were observed in the leukemia cell lines LOUCY and MEGAL. Overexpression of SET-Nup214 in HeLa cells leads to the formation of similar nuclear bodies that recruit CRM1, export cargo proteins, and certain nucleoporins and concomitantly affect nuclear protein and poly(A)(+) RNA export. SQSTM1-Nup214, although mostly cytoplasmic, also forms nuclear bodies and inhibits nuclear protein but not poly(A)(+) RNA export. The interaction of the fusion proteins with CRM1 is RanGTP-dependent, as shown in co-immunoprecipitation experiments and binding assays. Further analysis revealed that the Nup214 parts mediate the inhibition of nuclear export, whereas the SET or SQSTM1 part determines the localization of the fusion protein and therefore the extent of the effect. SET-Nup214 nuclear bodies are highly mobile structures, which are in equilibrium with the nucleoplasm in interphase and disassemble during mitosis or upon treatment of cells with the CRM1-inhibitor leptomycin B. Strikingly, we found that nucleoporins can be released from nuclear bodies and reintegrated into existing NPC. Our results point to nuclear bodies as a means of preventing the formation of potentially insoluble and harmful protein aggregates that also may serve as storage compartments for nuclear transport factors.

  15. Osteoclastic miR-214 targets TRAF3 to contribute to osteolytic bone metastasis of breast cancer

    Science.gov (United States)

    Liu, Jin; Li, Defang; Dang, Lei; Liang, Chao; Guo, Baosheng; Lu, Cheng; He, Xiaojuan; Cheung, Hilda Y. S.; He, Bing; Liu, Biao; Li, Fangfei; Lu, Jun; Wang, Luyao; Shaikh, Atik Badshah; Jiang, Feng; Lu, Changwei; Peng, Songlin; Zhang, Zongkang; Zhang, Bao-Ting; Pan, Xiaohua; Xiao, Lianbo; Lu, Aiping; Zhang, Ge

    2017-01-01

    The role of osteoclastic miRNAs in regulating osteolytic bone metastasis (OBM) of breast cancer is still underexplored. Here, we examined the expression profiles of osteoclastogenic miRNAs in human bone specimens and identified that miR-214-3p was significantly upregulated in breast cancer patients with OBM. Consistently, we found increased miR-214-3p within osteoclasts, which was associated with the elevated bone resorption, during the development of OBM in human breast cancer xenografted nude mice (BCX). Furthermore, genetic ablation of osteoclastic miR-214-3p in nude mice prevent the development of OBM. Conditioned medium from MDA-MB-231 cells dramatically stimulated miR-214-3p expression to promote osteoclast differentiation. Mechanistically, a series of in vitro study showed that miR-214-3p directly targeted Traf3 to promote osteoclast activity and bone-resorbing activity. In addition, osteoclast-specific miR-214-3p knock-in mice showed remarkably increased bone resorption when compared to the littermate controls, which was attenuated after osteoclast-targeted treatment with Traf3 3′UTR-containing plasmid. In BCX nude mice, osteoclast-targeted antagomir-214-3p delivery could recover the TRAF3 protein expression and attenuate the development of OBM, respectively. Collectively, inhibition of osteoclastic miR-214-3p may be a potential therapeutic strategy for breast cancer patients with OBM. Meanwhile, the intraosseous TRAF3 could be a promising biomarker for evaluation of the treatment response of antagomir-214-3p. PMID:28071724

  16. 49 CFR 214.517 - Retrofitting of existing on-track roadway maintenance machines manufactured on or after January 1...

    Science.gov (United States)

    2010-10-01

    ... TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.517... maintenance machine manufactured on or after January 1, 1991, shall have the following: (a) A change-of...

  17. SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines

    Directory of Open Access Journals (Sweden)

    Zaborski Margarete

    2009-01-01

    Full Text Available Abstract Background SET-NUP214 fusion resulting from a recurrent cryptic deletion, del(9(q34.11q34.13 has recently been described in T-cell acute lymphoblastic leukemia (T-ALL and in one case of acute myeloid leukemia (AML. The fusion protein appears to promote elevated expression of HOXA cluster genes in T-ALL and may contribute to the pathogenesis of the disease. We screened a panel of ALL and AML cell lines for SET-NUP214 expression to find model systems that might help to elucidate the cellular function of this fusion gene. Results Of 141 human leukemia/lymphoma cell lines tested, only the T-ALL cell line LOUCY and the AML cell line MEGAL expressed the SET(TAF-Iβ-NUP214 fusion gene transcript. RT-PCR analysis specifically recognizing the alternative first exons of the two TAF-I isoforms revealed that the cell lines also expressed TAF-Iα-NUP214 mRNA. Results of fluorescence in situ hybridization (FISH and array-based copy number analysis were both consistent with del(9(q34.11q34.13 as described. Quantitative genomic PCR also confirmed loss of genomic material between SET and NUP214 in both cell lines. Genomic sequencing localized the breakpoints of the SET gene to regions downstream of the stop codon and to NUP214 intron 17/18 in both LOUCY and MEGAL cells. Both cell lines expressed the 140 kDa SET-NUP214 fusion protein. Conclusion Cell lines LOUCY and MEGAL express the recently described SET-NUP214 fusion gene. Of special note is that the formation of the SET exon 7/NUP214 exon 18 gene transcript requires alternative splicing as the SET breakpoint is located downstream of the stop codon in exon 8. The cell lines are promising model systems for SET-NUP214 studies and should facilitate investigating cellular functions of the the SET-NUP214 protein.

  18. miR-214 down-regulates ARL2 and suppresses growth and invasion of cervical cancer cells.

    Science.gov (United States)

    Peng, Ruiqing; Men, Jianlong; Ma, Rui; Wang, Qian; Wang, Yang; Sun, Ying; Ren, Jing

    2017-03-11

    Increasing evidence has shown that miRNAs are implicated in carcinogenesis and can function as oncogenes or tumor suppressor genes in human cancers. In this study, we confirmed that miR-214 is frequently down-regulated in cervical cancer compared with normal cervical tissues. Ectopic expression of miR-214 suppressed proliferation, migration and invasion of HeLa and C33A cervical cancer cells. Bioinformatics analysis revealed that ADP ribosylation factor like 2 (ARL2) was a potential target of miR-214 and was remarkably up-regulated in cervical cancer. Knockdown of ARL2 markedly inhibited cervical cancer cell proliferation, migration and invasion, similarly to over-expression of miR-214, indicating that ARL2 may function as an oncogene in cervical cancer. In conclusion, our study revealed that miR-214 acts as a tumor suppressor via inhibiting proliferation, migration and invasion of cervical cancer cells through targeting ARL2, and that both miR-214 and ARL2 may serve as prognostic or therapeutic targets for cervical cancer.

  19. Leukemia-Associated Nup214 Fusion Proteins Disturb the XPO1-Mediated Nuclear-Cytoplasmic Transport Pathway and Thereby the NF-κB Signaling Pathway.

    Science.gov (United States)

    Saito, Shoko; Cigdem, Sadik; Okuwaki, Mitsuru; Nagata, Kyosuke

    2016-07-01

    Nuclear-cytoplasmic transport through nuclear pore complexes is mediated by nuclear transport receptors. Previous reports have suggested that aberrant nuclear-cytoplasmic transport due to mutations or overexpression of nuclear pore complexes and nuclear transport receptors is closely linked to diseases. Nup214, a component of nuclear pore complexes, has been found as chimeric fusion proteins in leukemia. Among various Nup214 fusion proteins, SET-Nup214 and DEK-Nup214 have been shown to be engaged in tumorigenesis, but their oncogenic mechanisms remain unclear. In this study, we examined the functions of the Nup214 fusion proteins by focusing on their effects on nuclear-cytoplasmic transport. We found that SET-Nup214 and DEK-Nup214 interact with exportin-1 (XPO1)/CRM1 and nuclear RNA export factor 1 (NXF1)/TAP, which mediate leucine-rich nuclear export signal (NES)-dependent protein export and mRNA export, respectively. SET-Nup214 and DEK-Nup214 decreased the XPO1-mediated nuclear export of NES proteins such as cyclin B and proteins involved in the NF-κB signaling pathway by tethering XPO1 onto nuclear dots where Nup214 fusion proteins are localized. We also demonstrated that SET-Nup214 and DEK-Nup214 expression inhibited NF-κB-mediated transcription by abnormal tethering of the complex containing p65 and its inhibitor, IκB, in the nucleus. These results suggest that SET-Nup214 and DEK-Nup214 perturb the regulation of gene expression through alteration of the nuclear-cytoplasmic transport system.

  20. MICROSTRUCTURAL EVOLUTION OF HIGHLY UNDERCOOLED EUTECTIC Nii78.6Si21.4 ALLOY

    Institute of Scientific and Technical Information of China (English)

    Y.P. Lu; G.C. Yang; C.L. Yang; H.P. Wang; Y.H. Zhou

    2006-01-01

    A large undercooling level up to 550K(0.386TE) was achieved in eutectic Ni78.6Si21.4 melt by the combination of molten-glass and cyclic superheating. A microcrystalline structure is obtained at large undercooling. Surprisingly, the morphology of α(Ni) phase transits from the non-faceted phase to faceted phase at large undercooling of 390K. Based on the classical nucleation theory and transient nucleation theory, the process of microstructure evolution and competitive nucleation was analyzed, and the refinement of crystal structure is determined by the high nucleation rate under large undercooling.

  1. Work in the area of buildings 513 - 289 - 214 - Disruption to traffic

    CERN Multimedia

    Optical Fibre Section, TS/EL

    2005-01-01

    The TS/EL group wishes to inform you that, in order to create an optical fibre access for the GEANT2 project, Route Rutherford will be closed to the public between Buildings 289 and 214 on 13 July 2005. Part of the Sophora car park will also be closed while the work is in progress (week 28: 11 - 15 July). You are requested to comply with the road signs in place. For more information, please call 160484. Optical Fibre Section, TS/EL

  2. 214例不孕症患者血清PRL测定

    Institute of Scientific and Technical Information of China (English)

    管京

    1995-01-01

    高泌乳素血症是引起不孕症的重要原因之一。放射免疫分析法测定血清PRL,能快速、准确地反映人体内PRL的水平。因此我们对214例不孕症患者进行了血清PRL的放射免疫法测定,现将结果报告如下。

  3. First Synthesis of (+)-2,14-Deoxyalatol from α-Santonin

    Institute of Scientific and Technical Information of China (English)

    XIA,Wu-Jiong(夏吾炯); SUN,Liang-Dong(孙良东); SHI,Lei(史雷); ZHANG,Shu-Yu(张书宇); TU,Yong-Qiang(涂永强)

    2004-01-01

    A novel and general approach for synthesis of the multi-oxygenated dihydrofuran sesquiterpenes has been developed starting from santonin.The key steps involve:the strategic acid-catalyzed double-bond shifting affording 4,the novel base-promoted epoxide rearrangement of 5 generating two key functionals(the C5-OH and the(7,11 double bond),and the stereoselective cyclization of tetrahydrofuran ring without pre-controlling the stereochemistry of C-7.As an example of this approach,synthesis of(+)-2,14-deoxyalatol was described in detail.

  4. Isotope shifts and hyperfine structure of {sup 212-214}Ra{sup +}

    Energy Technology Data Exchange (ETDEWEB)

    Giri, Gouri S.; Sahoo, Bijaya K.; Versolato, Oscar O.; Wansbeek, Lotje W.; Willmann, Lorenz; Wilschut, Hans W.; Jungmann, Klaus; Timmermans, Rob G.E. [Kernfysisch Versneller Instituut, University of Groningen (Netherlands)

    2010-07-01

    The availability of short lived Ra isotopes at the TRI{mu}P facility of the KVI makes the study of atomic structure of these isotopes possible. {sup 212-214}Ra ions are trapped in a buffer-gas filled Paul trap. Isotope shifts and hyperfine structures of {sup 212-214}Ra{sup +} for several transitions were obtained by laser spectroscopy. Ra{sup +} is of particular interest for a most precise measurement of Atomic Parity Violation (APV), currently underway in our group. Such a measurement will provide a stringent test of the Standard Model of particle physics. However, the interpretation of APV measurements require the determination of the weak matrix elements to better than 1% accuracy in order to extract the weak charge (Weinberg angle). Currently, our calculations are accurate to some 3%. We therefore plan to exploit the fact that radium has a wide range of isotopes by looking at the ratio E1APV/ E1'APV for two -or more- isotopes, and thus canceling atomic uncertainties. Here, nuclear structure effects start to play a role. The extracted isotope shifts and hyperfine interactions provide indispensable information. In addition, the measured hyperfine structure of {sup 213}Ra{sup +} is of particular interest for the proposed Radium single ion clock.

  5. A betatron tune fitting package for the Tevatron 21.4 MHz Schottky monitor

    CERN Document Server

    Lebrun, Paul; Todesco, Ezio; You, Jianming; Yuan, Zongwei

    2005-01-01

    Accurate control of the betatron tunes and chromaticities is required to optimize the dynamical aperture of any large synchrotron. The Fermilab Tevatron is equipped with two independent Schottky monitors, one operating at 21.4 MHz and the other 1.7 GHz. While the latter one allows us to characterize individual bunches separated by 396 ns, the former one has a larger Q and can give precise tune measurements. A new front-end and related data acquisition for this 21.4 MHz resonator has been installed and commissioned during the FY04 Collider RunII. Output signal are digitized at 100 KHz. Frequency spectra are transfered to dedicated server nodes and fitted in real time. Such frequency spectra are quite complex, due to inherent noise, horizontal/vertical coupling and synchrotron motion. Sophisticated fitting strategies are required. Optimization of this fitting package on relatively powerful commodity computer allows us to report tune and chromaticity measurements at almost 1 Hz. The architecture of the data acqu...

  6. CHSE-214: A model for studying extracellular dsRNA sensing in vitro.

    Science.gov (United States)

    Monjo, A L; Poynter, S J; DeWitte-Orr, S J

    2017-09-01

    Double-stranded RNA (dsRNA) is produced by almost all viruses during their replicative cycle and is a potent inducer of the innate antiviral immune response including inducing expression of type I interferons (IFNs) and interferon-stimulated genes (ISGs). During lytic virus infections intracellular dsRNA can escape into the extracellular space, where surface pattern recognition receptors, such as class A scavenger receptors (SR-As) facilitate its binding and entry into neighbouring cells. Studying extracellular dsRNA entry is difficult due to the ubiquitous expression profile and compensatory dsRNA binding characteristics of SR-As; a SR-A deficient cell line has yet to be identified. The present study suggests the Chinook salmon embryonic cell line, CHSE-214, as a model for studying extracellular dsRNA sensing in vitro. CHSE-214 is unable to bind and respond to extracellular dsRNA, can only respond to dsRNA when it is transfected into the cells, and is able to bind dsRNA when overexpressing human SR-AI. The applications for this model could include elucidating: dsRNA binding and entry mechanisms, including sequence and length effects, as well as SR-A and other putative surface dsRNA receptor ligand binding studies. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway.

    Science.gov (United States)

    Ahmed, Mohammed I; Alam, Majid; Emelianov, Vladimir U; Poterlowicz, Krzysztof; Patel, Ankit; Sharov, Andrey A; Mardaryev, Andrei N; Botchkareva, Natalia V

    2014-11-24

    Skin development is governed by complex programs of gene activation and silencing, including microRNA-dependent modulation of gene expression. Here, we show that miR-214 regulates skin morphogenesis and hair follicle (HF) cycling by targeting β-catenin, a key component of the Wnt signaling pathway. miR-214 exhibits differential expression patterns in the skin epithelium, and its inducible overexpression in keratinocytes inhibited proliferation, which resulted in formation of fewer HFs with decreased hair bulb size and thinner hair production. The inhibitory effects of miR-214 on HF development and cycling were associated with altered activities of multiple signaling pathways, including decreased expression of key Wnt signaling mediators β-catenin and Lef-1, and were rescued by treatment with pharmacological Wnt activators. Finally, we identify β-catenin as one of the conserved miR-214 targets in keratinocytes. These data provide an important foundation for further analyses of miR-214 as a key regulator of Wnt pathway activity and stem cell functions during normal tissue homeostasis, regeneration, and aging.

  8. Nuclear distributions of NUP62 and NUP214 suggest architectural diversity and spatial patterning among nuclear pore complexes.

    Directory of Open Access Journals (Sweden)

    Yayoi Kinoshita

    Full Text Available The shape of nuclei in many adherent cultured cells approximates an oblate ellipsoid, with contralateral flattened surfaces facing the culture plate or the medium. Observations of cultured cell nuclei from orthogonal perspectives revealed that nucleoporin p62 (NUP62 and nucleoporin 214 (NUP214 are differentially distributed between nuclear pore complexes on the flattened surfaces and peripheral rim of the nucleus. High resolution stimulated emission depletion (STED immunofluorescence microscopy resolved individual NPCs, and suggested both heterogeneity and microheterogeneity in NUP62 and NUP214 immunolabeling among in NPC populations. Similar to nuclear domains and interphase chromosome territories, architectural diversity and spatial patterning of NPCs may be an intrinsic property of the nucleus that is linked to the functions and organization of underlying chromatin.

  9. Inhibitors of Eicosanoid Biosynthesis Influencing the Transcripts Level of sHSP21.4 Gene Induced by Pathogen Infections, in Antheraea pernyi

    OpenAIRE

    Congfen Zhang; Lishang Dai; Lei Wang; Cen Qian; Guoqing Wei; Jun Li; Baojian Zhu; Chaoliang Liu

    2015-01-01

    Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherich...

  10. Mediated protective effect of electroacupuncture pretreatment by miR-214 on myocardial ischemia/reperfusion injury

    Institute of Scientific and Technical Information of China (English)

    Pei-Yu LIU; Yi TIAN; Shi-Yuan XU

    2014-01-01

    Background Electroacupuncture pretreatment plays a protective role in myocardial ischemia/reperfusion (I/R) injury and microRNAs (miRNAs) could act on various facets of cardiac function. However, the role of miRNAs in the cardioprotection by electroacupuncture pre-treatment on myocardial I/R injury remains unknown. The purpose of the study was to examine whether miR-214 was involved in cardio-protection by electroacupuncture. Methods Using rat myocardial I/R model, we examined the role of electroacupuncture pretreatment in myocardial I/R injury and analyzed the changes in the expression of miR-214. In addition, I/R was simulated in vitro by performing oxy-gen-glucose deprivation (OGD) on H9c2 cell cultures, and the effect of electroacupuncture pretreatment on I/R injury as well as expressional level of miR-214 were examined in vitro. Furthermore, the miR-214 mimic was transfected into OGD-treated H9c2 cells, we analyzed the cell apoptosis, lactate dehydrogenase (LDH) and creatine kinase (CK) activities, intracellular free Ca2+concentration ([Ca2+]i) as well as the relative protein levels of sodium/calcium exchanger 1(NCX1), BCL2-like 11 (BIM), calmodulin-dependent protein kinase IIδ(CaMKIIδ) and Cyclophilin D (CypD). Results The in vivo results revealed that compared with the I/R group, the electroacupuncture pretreatment group showed significant decreased myocardial infarct size, as well as the increased indices of the cardiac function, including heart rate, mean arterial pressure, left ventricular systolic pressure and maximal rate for left ventricular pressure rising and declining (±dp/dt max). In addition, electroacupuncture pretreatment could inhibit the elevation of LDH and CK activities induced by I/R injury. The quantitative PCR (qPCR) results demonstrated electroacupuncture pretreatment could provide cardioprotection against myocardial I/R injury in rats with miR-214 up-regulation. In the meanwhile, in vitro, electroacupuncture pretreatment protected H9

  11. Thermodynamic Critical Field and Superconducting Fluctuation of Vortices for High Temperature Cuprate Superconductor: La-214

    Energy Technology Data Exchange (ETDEWEB)

    Finnemore, Douglas K. [Iowa State Univ., Ames, IA (United States)

    2001-01-01

    Thermodynamics has been studied systematically for the high temperature cuprate superconductor La2-xSrxCuO4-δ, La-214, in the entire superconductive region from strongly underdoped to strongly overdoped regimes. Magnetization studies with H $\\parallel$ c have been made in order to investigate the changes in free energy of the system as the number of carriers is reduced. Above the superconducting transition temperature, the normal-state magnetization exhibits a two-dimensional Heisenberg antiferromagnetic behavior. Below Tc, magnetization data are thermodynamically reversible over large portions of the H-T plane, so the free energy is well defined in these regions. As the Sr concentration is varied over the wide range from 0.060 (strongly underdoped) to 0.234 (strongly overdoped), the free energy change goes through a maximum at the optimum doped in a manner similar to the Tc0 vs. x curve. The density of states, N(0), remains nearly constant in the overdoped and optimum doped regimes, taking a broad maximum around x = 0.188, and then drops abruptly towards zero in the underdoped regime. The La2-xSrxCuO4 (La-214) system displays the fluctuating vortex behavior with the characteristic of either 2D or 3D fluctuations as indicated by clearly identifiable crossing points T* close to Tc. The dimensional character of the fluctuations depends on both applied magnetic fields and the density of charge carriers. The dimensional crossover from 2D to 3D occurs in the strongly underdoped regime when the c-axis coherence distance ξc becomes comparable to the spacing between adjacent CuO2 layers s at sufficiently high magnetic field near Hc2.

  12. Solution structure and dynamics of the I214V mutant of the rabbit prion protein.

    Directory of Open Access Journals (Sweden)

    Yi Wen

    Full Text Available BACKGROUND: The conformational conversion of the host-derived cellular prion protein (PrP(C into the disease-associated scrapie isoform (PrP(Sc is responsible for the pathogenesis of transmissible spongiform encephalopathies (TSEs. Various single-point mutations in PrP(Cs could cause structural changes and thereby distinctly influence the conformational conversion. Elucidation of the differences between the wild-type rabbit PrP(C (RaPrP(C and various mutants would be of great help to understand the ability of RaPrP(C to be resistant to TSE agents. METHODOLOGY/PRINCIPAL FINDINGS: We determined the solution structure of the I214V mutant of RaPrP(C(91-228 and detected the backbone dynamics of its structured C-terminal domain (121-228. The I214V mutant displays a visible shift of surface charge distribution that may have a potential effect on the binding specificity and affinity with other chaperones. The number of hydrogen bonds declines dramatically. Urea-induced transition experiments reveal an obvious decrease in the conformational stability. Furthermore, the NMR dynamics analysis discloses a significant increase in the backbone flexibility on the pico- to nanosecond time scale, indicative of lower energy barrier for structural rearrangement. CONCLUSIONS/SIGNIFICANCE: Our results suggest that both the surface charge distribution and the intrinsic backbone flexibility greatly contribute to species barriers for the transmission of TSEs, and thereby provide valuable hints for understanding the inability of the conformational conversion for RaPrP(C.

  13. Thermodynamic Critical Field and Superconducting Fluctuation of Vortices for High Temperature Cuprate Superconductor: La-214

    Energy Technology Data Exchange (ETDEWEB)

    Douglas K. Finnemore

    2001-06-25

    Thermodynamics has been studied systematically for the high temperature cuprate superconductor La{sub 2-x}Sr{sub x}CuO{sub 4-{delta}}, La-214, in the entire superconductive region from strongly underdoped to strongly overdoped regimes. Magnetization studies with H {parallel} c have been made in order to investigate the changes in free energy of the system as the number of carriers is reduced. Above the superconducting transition temperature, the normal-state magnetization exhibits a two-dimensional Heisenberg antiferromagnetic behavior. Below T{sub c}, magnetization data are thermodynamically reversible over large portions of the H-T plane, so the free energy is well defined in these regions. As the Sr concentration is varied over the wide range from 0.060 (strongly underdoped) to 0.234 (strongly overdoped), the free energy change goes through a maximum at the optimum doped in a manner similar to the T{sub c0} vs. x curve. The density of states, N(0), remains nearly constant in the overdoped and optimum doped regimes, taking a broad maximum around x = 0.188, and then drops abruptly towards zero in the underdoped regime. The La{sub 2-x}Sr{sub x}CuO{sub 4} (La-214) system displays the fluctuating vortex behavior with the characteristic of either 2D or 3D fluctuations as indicated by clearly identifiable crossing points T* close to T{sub c}. The dimensional character of the fluctuations depends on both applied magnetic fields and the density of charge carriers. The dimensional crossover from 2D to 3D occurs in the strongly underdoped regime when the c-axis coherence distance {xi}{sub c} becomes comparable to the spacing between adjacent CuO{sub 2} layers s at sufficiently high magnetic field near H{sub c2}.

  14. Thermodynamic Critical Field and Superconducting Fluctuation of Vortices for High Temperature Cuprate Superconductor: La-214

    Energy Technology Data Exchange (ETDEWEB)

    Yung Moo Huh

    2001-05-01

    Thermodynamics has been studied systematically for the high temperature cuprate superconductor La{sub 2-x}Sr{sub x}CuO{sub 4-{delta}}, La-214, in the entire superconductive region from strongly underdoped to strongly overdoped regimes. Magnetization studies with H{parallel}c have been made in order to investigate the changes in free energy of the system as the number of carriers is reduced. Above the superconducting transition temperature, the normal-state magnetization exhibits a two-dimensional Heisenberg antiferromagnetic behavior. Below T{sub c}, magnetization data are thermodynamically reversible over large portions of the H-T plane, so the free energy is well defined in these regions. As the Sr concentration is varied over the wide range from 0.060 (strongly underdoped) to 0.234 (strongly overdoped), the free energy change goes through a maximum at the optimum doped in a manner similar to the T{sub c0} vs. x curve. The density of states, N(0), remains nearly constant in the overdoped and optimum doped regimes, taking a broad maximum around x = 0.188, and then drops abruptly towards zero in the underdoped regime. The La{sub 2-x}Sr{sub x}CuO{sub 4} (La-214) system displays the fluctuating vortex behavior with the characteristic of either 2D or 3D fluctuations as indicated by clearly identifiable crossing points T* close to T{sub c}. The dimensional character of the fluctuations depends on both applied magnetic fields and the density of charge carriers. The dimensional crossover from 2D to 3D occurs in the strongly underdoped regime when the c-axis coherence distance {zeta}{sub c} becomes comparable to the spacing between adjacent CuO{sub 2} layers s at sufficiently high magnetic fields near H{sub c2}.

  15. 76 FR 72241 - Fourteenth Meeting: RTCA Special Committee 214/EUROCAE WG-78: Standards for Air Traffic Data...

    Science.gov (United States)

    2011-11-22

    ... for Air Traffic Data Communication Services AGENCY: Federal Aviation Administration (FAA), U.S... Traffic Data Communication Services meeting. SUMMARY: The FAA is issuing this notice to advise the public of a meeting of RTCA Special Committee 214/EUROCAE WG-78: Standards for Air Traffic Data...

  16. 20 CFR 416.214 - You are disabled and drug addiction or alcoholism is a contributing factor material to the...

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false You are disabled and drug addiction or....214 You are disabled and drug addiction or alcoholism is a contributing factor material to the... because you are disabled and drug addiction or alcoholism is a contributing factor material to...

  17. 48 CFR 52.214-23 - Late submissions, modifications, revisions, and withdrawals of technical proposals under two-step...

    Science.gov (United States)

    2010-10-01

    ..., modifications, revisions, and withdrawals of technical proposals under two-step sealed bidding. 52.214-23... Late submissions, modifications, revisions, and withdrawals of technical proposals under two-step..., Modifications, Revisions, and Withdrawals of Technical Proposals Under Two-Step Sealed Bidding (NOV 1999)...

  18. SCN1Bb R214Q found in 3 patients: 1 with Brugada syndrome and 2 with lone atrial fibrillation

    DEFF Research Database (Denmark)

    Olesen, Morten S; Holst, Anders G; Haunsø, Stig

    2012-01-01

    BACKGROUND: SCN1Bb encodes the ß-subunit of the sodium channel. A mutation in SCN1Bb R214Q has recently been shown both to increase the Kv4.3 current and to decrease the sodium current. The variant was suggested to increase the susceptibility to Brugada syndrome (BrS). OBJECTIVE: To sequence...

  19. MicroRNA-214 and MicroRNA-126 Are Potential Biomarkers for Malignant Endothelial Proliferative Diseases

    Directory of Open Access Journals (Sweden)

    Kazuki Heishima

    2015-10-01

    Full Text Available Malignant endothelial proliferative diseases including human angiosarcoma (AS and canine hemangiosarcoma (HSA are serious diseases with a grave prognosis. Establishing liquid biopsy-based biomarkers for screening has definite clinical utility; however, plasma miRNAs up- or down-regulated in these sarcomas have been unclear. For identifying possible diagnostic plasma miRNAs for these sarcomas, we investigated whether plasma miR-214 and miR-126, which miRNAs play important roles in angiogenesis and tumorigenesis, were elevated in malignant endothelial proliferative diseases. For this investigation, human angiosarcoma and canine hemangiosarcoma cell lines and clinical plasma samples of canine hemangiosarcoma were examined by performing miRNA qRT-PCR. We report here that human angiosarcoma and canine hemangiosarcoma cell lines over-secreted miR-214 and miR-126 via microvesicles; in addition, their levels in the plasma samples from canines with hemangiosarcoma were increased. Moreover, the surgical resection of primary tumors decreased the levels of plasma miR-214 and miR-126. Our findings suggest that these malignant endothelial proliferative diseases over-secreted miR-214 and miR-126, thus suggesting that these miRNAs have potential as diagnostic biomarkers for malignant endothelial proliferative diseases in canine and possible in human angiosarcoma.

  20. 8 CFR 214.12 - Preliminary enrollment of schools in the Student and Exchange Visitor Information System (SEVIS).

    Science.gov (United States)

    2010-01-01

    ... eligible schools to apply for preliminary enrollment through the Internet is as follows: (1) Eligible institutions must access the Internet site, http://www.ins.usdoj.gov/sevis. Upon accessing the site, the... DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS NONIMMIGRANT CLASSES § 214.12 Preliminary...

  1. Inhibitors of eicosanoid biosynthesis influencing the transcripts level of sHSP21.4 gene induced by pathogen infections, in Antheraea pernyi.

    Science.gov (United States)

    Zhang, Congfen; Dai, Lishang; Wang, Lei; Qian, Cen; Wei, Guoqing; Li, Jun; Zhu, Baojian; Liu, Chaoliang

    2015-01-01

    Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus (NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.

  2. 49 CFR 214.353 - Training and qualification of roadway workers who provide on-track safety for roadway work groups.

    Science.gov (United States)

    2010-10-01

    ... provide on-track safety for roadway work groups. 214.353 Section 214.353 Transportation Other Regulations... who provide on-track safety for roadway work groups. (a) The training and qualification of roadway workers who provide for the on-track safety of groups of roadway workers through establishment of...

  3. Impact of HIV-1 reverse transcriptase polymorphism F214L on virological response to thymidine analogue based regimens in ART-naïve and experienced patients

    DEFF Research Database (Denmark)

    Silberstein, F; Cozzi-Lepri, A; Ruiz, L

    2007-01-01

    BACKGROUND: A negative association between the polymorphism F214L and type 1 thymidine analogue (TA) mutations (TAMs) has been observed. However, the virological response to TAs according to the detection of F214L has not been evaluated. METHODS: We studied 590 patients from EuroSIDA who started ...

  4. Thermodynamic Critical Field and Superconducting Fluctuation of Vortices for High Temperature Cuprate Superconductor La-214

    CERN Document Server

    Yung Moo Hu

    2001-01-01

    charge carriers. The dimensional crossover from 2D to 3D occurs in the strongly underdoped regime when the c-axis coherence distance zeta sub c becomes comparable to the spacing between adjacent CuO sub 2 layers s at sufficiently high magnetic fields near H sub c sub 2. Thermodynamics has been studied systematically for the high temperature cuprate superconductor La sub 2 sub - sub x Sr sub x CuO sub 4 sub - subdelta, La-214, in the entire superconductive region from strongly underdoped to strongly overdoped regimes. Magnetization studies with H(parallel)c have been made in order to investigate the changes in free energy of the system as the number of carriers is reduced. Above the superconducting transition temperature, the normal-state magnetization exhibits a two-dimensional Heisenberg antiferromagnetic behavior. Below T sub c , magnetization data are thermodynamically reversible over large portions of the H-T plane, so the free energy is well defined in these regions. As the Sr concentration is varied ove...

  5. System for Automated Geoscientific Analyses (SAGA v. 2.1.4

    Directory of Open Access Journals (Sweden)

    O. Conrad

    2015-02-01

    Full Text Available The System for Automated Geoscientific Analyses (SAGA is an open-source Geographic Information System (GIS, mainly licensed under the GNU General Public License. Since its first release in 2004, SAGA has rapidly developed from a specialized tool for digital terrain analysis to a comprehensive and globally established GIS platform for scientific analysis and modeling. SAGA is coded in C++ in an object oriented design and runs under several operating systems including Windows and Linux. Key functional features of the modular organized software architecture comprise an application programming interface for the development and implementation of new geoscientific methods, an easily approachable graphical user interface with many visualization options, a command line interpreter, and interfaces to scripting and low level programming languages like R and Python. The current version 2.1.4 offers more than 700 tools, which are implemented in dynamically loadable libraries or shared objects and represent the broad scopes of SAGA in numerous fields of geoscientific endeavor and beyond. In this paper, we inform about the system's architecture, functionality, and its current state of development and implementation. Further, we highlight the wide spectrum of scientific applications of SAGA in a review of published studies with special emphasis on the core application areas digital terrain analysis, geomorphology, soil science, climatology and meteorology, as well as remote sensing.

  6. System for Automated Geoscientific Analyses (SAGA) v. 2.1.4

    Science.gov (United States)

    Conrad, O.; Bechtel, B.; Bock, M.; Dietrich, H.; Fischer, E.; Gerlitz, L.; Wehberg, J.; Wichmann, V.; Böhner, J.

    2015-07-01

    The System for Automated Geoscientific Analyses (SAGA) is an open source geographic information system (GIS), mainly licensed under the GNU General Public License. Since its first release in 2004, SAGA has rapidly developed from a specialized tool for digital terrain analysis to a comprehensive and globally established GIS platform for scientific analysis and modeling. SAGA is coded in C++ in an object oriented design and runs under several operating systems including Windows and Linux. Key functional features of the modular software architecture comprise an application programming interface for the development and implementation of new geoscientific methods, a user friendly graphical user interface with many visualization options, a command line interpreter, and interfaces to interpreted languages like R and Python. The current version 2.1.4 offers more than 600 tools, which are implemented in dynamically loadable libraries or shared objects and represent the broad scopes of SAGA in numerous fields of geoscientific endeavor and beyond. In this paper, we inform about the system's architecture, functionality, and its current state of development and implementation. Furthermore, we highlight the wide spectrum of scientific applications of SAGA in a review of published studies, with special emphasis on the core application areas digital terrain analysis, geomorphology, soil science, climatology and meteorology, as well as remote sensing.

  7. Electron beam welding of 8-inch thick 2-1/4 Cr-1 Mo. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Weber, Charles M.

    1980-08-01

    Electron beam welding procedures were developed and used to make sound welds in 8-inch thick 2-1/4 Cr-1 Mo in the horizontal position. A two-pass technique, one pass from each side, was developed for welding the 8-inch thickness. Techniques for eliminating various weld defects were developed. It was learned that the beam oscillation conditions strongly influenced welding performance. Procedures were developed for hard and soft vacuum operation, but hard vacuum was preferred. Procedures for starting and stopping the welding sequence were developed, along with a repair technique involving re-welding over a plug filled hole. The joint fit-up requirements were determined: a joint mismatch of 3/4 in. was welded, and a joint gap opening of 0.100 in. was welded without alteration of the welding procedure. It was shown that it is not necessary to demagnetize the material for successful welding, but that a special magnetic shield may be needed to protect the electron beam from stray magnetic fields. A demonstration weld failed to meet the NDE requirements of the ASME Boiler and Pressure Vessel Code due to poor base metal quality which adversely affected weld performance. The mechanical properties (hardness, strength, ductility, and impact), and the microstructure of electron beam welded 8-inch thick SA387 Grade 22 Class 2 were determined and appeared to be adequate.

  8. Coordination chemistry of 1,4-bis-carboxymethylcyclam, H(2)(1,4-bcc).

    Science.gov (United States)

    Tonei, Deborah M; Ware, David C; Brothers, Penelope J; Plieger, Paul G; Clark, George R

    2006-01-07

    Zinc metal reduction of the cobalt(III) complex [Co(1,4-bcc)](+) (1,4-bcc = 1,4-bis-carboxymethylcyclam) produces the corresponding cobalt(II) complex which crystallises as the coordination polymer {[Co(1,4-bcc)]ZnCl(2)}(n). A method has been developed for removal of the cobalt(III) ion from [Co(1,4-bcc)](+) and isolation of the free ligand as its hydrochloride salt, H(2)(1,4-bcc).4HCl. This has been used for the preparation of new metal complexes, and the syntheses and characterisation of the copper(ii), nickel(ii), zinc(ii) and chromium(iii) complexes containing the 1,4-bcc ligand are described. X-Ray crystal structures of {[Co(1,4-bcc)]ZnCl(2)}(n).2.5H(2)O, {[Cu(1,4-bcc)]CuCl(2)}(n).0.25MeOH.H(2)O and [Cu(1,4-bcc)H]ClO(4) show the complexes to have the trans(O) geometry of the 1,4-bcc ligand, while the structure of [Cr(1,4-bcc)H(0.5)](ClO(4))(1.5).EtOH exhibits the cis(O) configuration.

  9. System for Automated Geoscientific Analyses (SAGA) v. 2.1.4

    Science.gov (United States)

    Conrad, O.; Bechtel, B.; Bock, M.; Dietrich, H.; Fischer, E.; Gerlitz, L.; Wehberg, J.; Wichmann, V.; Böhner, J.

    2015-02-01

    The System for Automated Geoscientific Analyses (SAGA) is an open-source Geographic Information System (GIS), mainly licensed under the GNU General Public License. Since its first release in 2004, SAGA has rapidly developed from a specialized tool for digital terrain analysis to a comprehensive and globally established GIS platform for scientific analysis and modeling. SAGA is coded in C++ in an object oriented design and runs under several operating systems including Windows and Linux. Key functional features of the modular organized software architecture comprise an application programming interface for the development and implementation of new geoscientific methods, an easily approachable graphical user interface with many visualization options, a command line interpreter, and interfaces to scripting and low level programming languages like R and Python. The current version 2.1.4 offers more than 700 tools, which are implemented in dynamically loadable libraries or shared objects and represent the broad scopes of SAGA in numerous fields of geoscientific endeavor and beyond. In this paper, we inform about the system's architecture, functionality, and its current state of development and implementation. Further, we highlight the wide spectrum of scientific applications of SAGA in a review of published studies with special emphasis on the core application areas digital terrain analysis, geomorphology, soil science, climatology and meteorology, as well as remote sensing.

  10. [The metabolism of [2-(14)C] glycine in rat tissues in vivo].

    Science.gov (United States)

    Brodin, S V; Ianovych, V H

    1996-01-01

    It is found that two hours after intraperitoneal injection of [2-(14)C] glycine to rats its higher amount (83.6%) is used in the protein synthesis and 16.4% is catabolized. The carbonic chain of the amino acid is used to the greater extent in the synthesis of lipids than in the oxidation and glucogenesis. Radioactivity of the proteins in the investigated organs and tissues of rats varies within L7-50.6 thou. DPM per 100 mg of wet tissue and decreases in a series: small intestine mucosa, kidneys, liver, stomach mucosa, heart, lungs, cardiac muscle, skin, skeletal muscle, adipose tissue, brain. Radioactivity of lipids is within the limits of 0.2-5.1 thou. DPM per 100 mg of wet tissue 11.7-81.6% of protein radioactivity) and decreases in a series: liver, adipose tissue, kidneys, lungs, cardiac muscle, stomach mucosa, skin, skeletal muscle, brain. The total radioactivity of glucose+glycogen varies within 0.05-0.34 thou. DPM per 100 mg of wet tissue (0.8-10.7% of protein radioactivity) and decreases is a series: kidneys, liver, brain, skeletal muscle.

  11. MiR-214 inhibits cell migration, invasion and promotes the drug sensitivity in human cervical cancer by targeting FOXM1.

    Science.gov (United States)

    Wang, Jian-Mei; Ju, Bao-Hui; Pan, Cai-Jun; Gu, Yan; Li, Meng-Qi; Sun, Li; Xu, Yan-Ying; Yin, Li-Rong

    2017-01-01

    MicroRNAs (miRNAs) play key roles in progression of cervical cancer. In the present study, we investigated the role of miR-214 in the process of migration, invasion and drug sensitivity to cisplatin in cervical cancer. We detected the differential expression of miR-214 in 19 cases cervical cancer tissues and normal tissues as well as 4 cervical cancer cells and one normal cervical cells by Real-time PCR. Then, wound healing assay, transwell invasion assay and MTT were used to detect the effects of migration, invasion and sensitivity to cisplatin of cervical cancer when miR-214 was overexpressed. Western blot, immunofluorescence and Flow Cytometry were used to detect the mechanism of migration, invasion and sensitivity to cisplatin. Next, bioinformatics analysis was used to find the target of miR-214. Through the luciferase reporter assay, Real-time PCR and western blot, we confirmed the binding relationship of miR-214 and FOXM1. In cervical cancer tissues, the expression of FOXM1 was detected by western blot and Immunohistochemistry. We also knocked down FOXM1 in cervical cancer cells, wound healing assay, transwell invasion assay and MTT were performed to detect the migration, invasion and sensitivity to cisplatin abilities of FOXM1. Western blot and Flow Cytometry were used to detect the mechanism of migration, invasion and sensitivity to cisplatin by FOXM1. Finally, we performed rescue expriments to confirm the function relationship between miR-214 and FOXM1. 1. Our results showed that miR-214 was frequently downregulated in tumor tissues and cancer cells especially in CIN III and cervical cancer stages. 2. Overexpression of miR-214 significantly inhibited migration and invasion of cervical cancer cells and prompted the sensitivity to cisplatin. 3. FOXM1 was identified as a target of miR-214 and down-regulated by miR-214. 4. Knocking down FOXM1 could inhibited migration and invasion of cervical cancer cells and prompted the sensitivity to cisplatin. 5. FOXM1 was

  12. ER stress negatively modulates the expression of the miR-199a/214 cluster to regulates tumor survival and progression in human hepatocellular cancer.

    Directory of Open Access Journals (Sweden)

    Quanlu Duan

    Full Text Available BACKGROUND: Recent studies have emphasized causative links between microRNAs (miRNAs deregulation and tumor development. In hepatocellular carcinoma (HCC, more and more miRNAs were identified as diagnostic and prognostic cancer biomarkers, as well as additional therapeutic tools. This study aimed to investigate the functional significance and regulatory mechanism of the miR-199a2/214 cluster in HCC progression. METHODS AND FINDINGS: In this study, we showed that miR-214, as well as miR-199a-3p and miR-199a-5p levels were significantly reduced in the majority of examined 23 HCC tissues and HepG2 and SMMC-7721 cell lines, compared with their nontumor counterparts. To further explore the role of miR-214 in hepatocarcinogenesis, we disclosed that the ER stress-induced pro-survival factor XBP-1 is a target of miR-214 by using western blot assay and luciferase reporter assay. Re-expression of miR-214 in HCC cell lines (HepG2 and SMMC-7721 inhibited proliferation and induced apoptosis. Furthermore, ectopic expression of miR-214 dramatically suppressed the ability of HCC cells to form colonies in vitro and to develop tumors in a subcutaneous xenotransplantation model of the BALB/c athymic nude mice. Moreover, reintroduction of XBP-1s attenuated miR-214-mediated suppression of HCC cells proliferation, colony and tumor formation. To further understand the mechanism of the miR-199a/214 cluster down-expression in HCC, we found that thapsigargin (TG and tunicamycin (TM or hypoxia-induced unfolded protein response (UPR suppresses the expression of the miR-199a/214 cluster in HCC cells. By promoter analysis of the miR-199a2/214 gene, we conjectured NFκB as a potential negative regulator. We further found that UPR and LPS-induced NFκB activation suppressed miR-199a2/214 transcription, and this suppression was reversed by NFκB inhibition in HCC cells. CONCLUSIONS: Our study suggest that modulation of miR-214 levels may provide a new therapeutic approach for

  13. Impact of HIV-1 reverse transcriptase polymorphism F214L on virological response to thymidine analogue-based regimens in antiretroviral therapy (ART)-naive and ART-experienced patients

    DEFF Research Database (Denmark)

    Ceccherini-Silberstein, Francesca; Cozzi-Lepri, Alessandro; Ruiz, Lidia

    2007-01-01

    A negative association between the polymorphism F214L and type 1 thymidine analogue (TA) mutations (TAMs) has been observed. However, the virological response to TAs according to the detection of F214L has not been evaluated....

  14. [Expression of SET-NUP214 fusion gene in patients with T-cell acute lymphoblastic leukemia and its clinical significance].

    Science.gov (United States)

    Dai, Hai-Ping; Wang, Qian; Wu, Li-Li; Ping, Na-Na; Wu, Chun-Xiao; Xie, Jun-Dan; Pan, Jin-Lan; Xue, Yong-Quan; Wu, De-Pei; Chen, Su-Ning

    2012-10-01

    This study was aimed to investigate the occurrence and clinical significance of the SET-NUP214 fusion gene in patients with T-cell acute lymphoblastic leukemia (T-ALL), analyse clinical and biological characteristics in this disease. RT-PCR was used to detect the expression of SET-NUP214 fusion gene in 58 T-ALL cases. Interphase FISH and Array-CGH were used to detect the deletion of 9q34. Direct sequencing was applied to detect mutations of PHF6 and NOTCH1. The results showed that 6 out of 58 T-ALL cases (10.3%) were detected to have the SET-NUP214 fusion gene by RT-PCR. Besides T-lineage antigens, expression of CD13 and(or) CD33 were detected in all the 6 cases. Deletions of 9q34 were detected in 4 out of the 6 patients by FISH. Array-CGH results of 3 SET-NUP214 positive T-ALL patients confirmed that this fusion gene was resulted from a cryptic deletion of 9q34.11q34.13. PHF6 and NOTCH1 gene mutations were found in 4 and 5 out of 6 SET-NUP214 positive T-ALL patients, respectively. It is concluded that SET-NUP214 fusion gene is often resulted from del(9)(q34). PHF6 and NOTCH1 mutations may be potential leukemogenic event in SET-NUP214 fusion gene.

  15. Detection of Helicobacter pylori infection by examination of human breath odor using electronic nose Bloodhound-214ST

    Science.gov (United States)

    Shnayder, E. P.; Moshkin, M. P.; Petrovskii, D. V.; Shevela, A. I.; Babko, A. N.; Kulikov, V. G.

    2009-05-01

    Our aim was to examine the possibility of use e-nose Bloodhound-214ST to determine presence or absence of H. pylori infection using exhalation samples of patients. Breath samples were collected twice: at baseline and after oral administration of 500 mg of urea. H. pylori status of patients was confirmed by antral biopsy. Using two approaches for the data analysis we showed the possibility to distinguish H. pylori free and infected patients.

  16. Psychiatric stigma in treatment seeking adults with personality problems: evidence from a sample of 214 patients.

    Directory of Open Access Journals (Sweden)

    Kirsten eCatthoor

    2015-07-01

    Full Text Available Stigmatization is a major hindrance in adult psychiatric patients with Axis-I diagnoses, as shown consistently in most studies. Significantly fewer studies on the emergence of psychiatric stigma in adult patients with personality disorders exist, although the resulting evidence is conclusive. Some authors consider patients with personality disorders at risk for severe stigmatization because of intense difficulties during interpersonal contact, even in a psychotherapeutic relationship. The aim of this study was primarily the assessment of pre-existing stigma in patients referred for intensive treatment for personality disorders. The study enrolled 214 patients admitted to the adult department of a highly specialized mental health care institute offering psychotherapy for patients with severe and complex personality pathology. All patients underwent a standard assessment with self-report questionnaires and a semi-structured interview to measure Axis II personality disorders. The Stigma Consciousness Questionnaire (SCQ and the Perceived Devaluation-Discrimination Questionnaire (DDQ, both validated instruments, were used to measure perceived and actual experiences of stigma. Independent sample t-tests were used to investigate differences in the mean total stigma scores for patients both with and without a personality disorder. One-way ANOVA’s were performed to assess the differences between having a borderline personality disorder, another personality disorder, or no personality disorder diagnosis.Multiple regression main effect analyses were conducted in order to explore the impact of the different personality disorder diagnosis on the level of stigma. The mean scores across all patient groups were consistent with rather low stigma. No differences were found for patients with or without a personality disorder diagnosis. Level of stigma in general was not associated with an accumulating number of personality disorders.

  17. Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus

    Directory of Open Access Journals (Sweden)

    Kibenge Frederick SB

    2005-08-01

    Full Text Available Abstract Infectious salmon anaemia (ISA is a highly fatal viral disease affecting marine-farmed Atlantic salmon which is caused by ISA virus (ISAV, a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae. Mx proteins are among the interferon (IFN-induced proteins responsible for the development of an antiviral state in vertebrate cells. We used real-time reverse transcription-polymerase chain reaction (RT-PCR and Chinook salmon embryo (CHSE-214 cells constitutively expressing Atlantic salmon Mx1 protein (ASMx1 to examine the antiviral properties of ASMx1 against two ISAV strains, NBISA01 and HKS-36, having phenotypically different growth properties (cytopathic vs non-cytopathic in the CHSE-214 cell line. We present evidence that ISAV is sensitive to ASMx1. CHSE-214 cells constitutively expressing ASMx1 showed increased resistance to infection with the cytopathic ISAV strain NBISA01, manifested as delayed development of cytopathic effects (CPE and significant reduction in the severity of CPE, as well as a 10-fold reduction in virus yield. However, by real-time RT-PCR we observed no significant difference in the mean threshold cycle (Ct values of ISAV RNA levels, suggesting that the ASMx1 activity on ISAV occurs at the post-transcription steps of virus replication, possibly in the cytoplasm.

  18. Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to infectious salmon anaemia virus.

    Science.gov (United States)

    Kibenge, Molly J T; Munir, Khalid; Kibenge, Frederick S B

    2005-08-26

    Infectious salmon anaemia (ISA) is a highly fatal viral disease affecting marine-farmed Atlantic salmon which is caused by ISA virus (ISAV), a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae. Mx proteins are among the interferon (IFN)-induced proteins responsible for the development of an antiviral state in vertebrate cells. We used real-time reverse transcription-polymerase chain reaction (RT-PCR) and Chinook salmon embryo (CHSE-214) cells constitutively expressing Atlantic salmon Mx1 protein (ASMx1) to examine the antiviral properties of ASMx1 against two ISAV strains, NBISA01 and HKS-36, having phenotypically different growth properties (cytopathic vs non-cytopathic) in the CHSE-214 cell line. We present evidence that ISAV is sensitive to ASMx1. CHSE-214 cells constitutively expressing ASMx1 showed increased resistance to infection with the cytopathic ISAV strain NBISA01, manifested as delayed development of cytopathic effects (CPE) and significant reduction in the severity of CPE, as well as a 10-fold reduction in virus yield. However, by real-time RT-PCR we observed no significant difference in the mean threshold cycle (Ct) values of ISAV RNA levels, suggesting that the ASMx1 activity on ISAV occurs at the post-transcription steps of virus replication, possibly in the cytoplasm.

  19. Laser and decay spectroscopy of the short-lived isotope 214Fr in the vicinity of the N =126 shell closure

    Science.gov (United States)

    Farooq-Smith, G. J.; Cocolios, T. E.; Billowes, J.; Bissell, M. L.; Budinčević, I.; Day Goodacre, T.; de Groote, R. P.; Fedosseev, V. N.; Flanagan, K. T.; Franchoo, S.; Garcia Ruiz, R. F.; Heylen, H.; Li, R.; Lynch, K. M.; Marsh, B. A.; Neyens, G.; Rossel, R. E.; Rothe, S.; Stroke, H. H.; Wendt, K. D. A.; Wilkins, S. G.; Yang, X. F.

    2016-11-01

    Combined decay and laser spectroscopy measurements of the isotope 214Fr are reported, using the collinear resonance ionization spectroscopy (CRIS) technique at the ISOLDE facility at CERN. For the Iπ=( 1- ) spin assignment, the g -factor value of g (214Fr)=+0.241 (16 ) corresponds to a relatively pure (π 1 h9 /2 ⊗ ν 2 g9 /2 ) ground-state configuration. An alternative interpretation with g (214Fr)=+0.144 (10 ) , for a (2-) spin assignment suggests a greater contribution of configuration mixing with the ν 1 i11 /2 orbital. As the N =126 shell closure is passed, a kink is observed at the δ 213 ,214 value, which is analogous to the behavior observed in the neighboring isotopic chains.

  20. Alpha-subunit positions methionine 180 and glutamate 214 of Pseudomonas stutzeri OX1 toluene-o-xylene monooxygenase influence catalysis.

    Science.gov (United States)

    Vardar, Gönül; Wood, Thomas K

    2005-02-01

    Alpha-subunit position M180 of toluene-o-xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o-cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p-nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.

  1. 结直肠癌中microRNA-214的表达及其与p53的关系

    Institute of Scientific and Technical Information of China (English)

    赵增仁; 樊智彬; 张丽静; 吴晨鹏; 张学明; 刘博

    2013-01-01

    目的 探讨结直肠癌中microRNA-214(miR-214)的表达及其与p53的关系.方法 采用实时荧光定量聚合酶链反应的方法检测结直肠癌患者及结肠癌细胞株野生型HCT-116 p53+/+和突变型HCT-116 p53-/-中miR-214的表达情况,同时在结肠癌患者中检测p53的表达.结果 miR-214在癌旁正常黏膜中的表达明显高于癌组织(P=0.021),p53在癌旁正常黏膜中的表达明显低于癌组织;miR-214在HCT-116 p53-/-细胞株中的表达显著高于HCT-116 p53+/+(P=0.028),与p53的表达情况相反;在结直肠癌组织中miR-214与p53无明显相关性(rs=-0.156,P=0.409).结论 在结直肠癌的发生发展中miR-214可能通过调节p53 发挥抑癌基因的功能,但尚未发现miR-214与p53存在明显相关性.

  2. Transfección de la línea celular de salmón CHSE-214 usando un liposoma no comercial Transfection of salmon cellular line CHSE-214 using a non-commercial liposome

    Directory of Open Access Journals (Sweden)

    M Aranda

    2007-01-01

    Full Text Available El rápido crecimiento de la acuicultura chilena ha incentivado la exploración de estrategias innovadoras de prevención sanitaria. Las vacunas de ADN se presentan como una alternativa viable para el control de enfermedades, pero existen barreras técnicas, como la vía de administración y los sistemas de transporte y entrega que necesitan ser soslayados. Los liposomas catiónicos son utilizados ampliamente en la incorporación de genes en líneas celulares mamíferas pero su alto costo hace prohibitivo su uso en escala comercial. Con el fin de abordar la problemática económica en la obtención del liposoma y para contribuir con un procedimiento de transfección in vitro en células de salmón, los objetivos de este trabajo fueron formular un liposoma a partir de polvo liofilizado de DOTAP y desarrollar un protocolo de transfección de vectores eucarióticos en la línea celular CHSE-214. Para ello se utilizaron los vectores pcDNA 4.0 HisMax I TOPO y pIRES-EGFP y la línea celular de salmón Chinook CHSE-214 (ATCC 1681, estableciendo tanto las características del liposoma formulado como las condiciones óptimas de transfección. Los resultados indicaron que el liposoma formulado desde polvo liofilizado de DOTAP comparte todas las características de los liposomas comerciales con la ventaja de poseer bajo costo, aproximadamente 10 veces menos. Con este liposoma se logró establecer un protocolo óptimo de transfección de células de peces CHSE-214, con vectores eucarióticos, no mostrando signos de toxicidad y permitiendo la expresión del gen incorporado en ambos vectores.The fast growth of Chilean aquaculture has impelled the exploration of new strategies of sanitary prevention. DNA vaccines appear as a feasible alternative for disease control, however there are technical barriers such as the form of administration and the carrier systems which need to be resolved. Liposomes are widely used for gene delivery in mammalian cell lines, but

  3. Precaution of self-ignite in -214 hydraulicking working area in shaft of Zazi coal mine%砟子矿竖井-214水采区自燃火灾防治技术

    Institute of Scientific and Technical Information of China (English)

    王升宇

    2004-01-01

    介绍了通化矿务局砟子煤矿竖井倾斜和急倾斜煤层水力采煤、-214采区自燃火灾情况,对其自然发火原因进行了分析,对单一倾斜煤层和煤层群水力采煤采空区煤炭自燃的防治,制定了技术和管理措施,为水采矿井防治采空区煤炭自然发火提供了思路和途径.

  4. Esclerose múltipla: análise clínica e evolutiva de 214 casos

    OpenAIRE

    Charles Peter Tilbery; Eduardo Felipe; Cristine Mella Baldauf; Peres,Mário Fernando P.

    1995-01-01

    Os autores apresentam uma série de 214 casos de esclerose múltipla provenientes de levantamento retrospectivo de prontuários na Disciplina de Neurologia da Faculdade de Ciências Médicas da Santa Casa de São Paulo e da clínica privada, no período de 1980 a 1993. Os dados encontrados foram analisados quanto a sexo, raça, média de idade de início dos sintomas, sintomas iniciais e sintomas evolutivos da doença. Os resultados foram analisados comparativamente aos encontrados em outras séries nacio...

  5. Alfacalcidol in men with osteoporosis: a prospective, observational, 2-year trial on 214 patients.

    Science.gov (United States)

    Ringe, J D; Farahmand, P; Schacht, E

    2013-03-01

    Due to pleiotropic-synergistic actions on bone, muscle, gut, brain and different other non-skeletal tissues, alfacalcidol is an interesting drug for treating osteoporosis. In studies on glucocorticoid-induced osteoporosis, men have always been treated with calcitriol or this active D-hormone prodrug, but there is no study of male patients only in the literature. The AIM-Trial (Alfacalcidol In Men) is an extension of the control group (n = 158) of our former risedronate study in male osteoporosis (Ringe et al. in Rheumatol Int 29:311-315, 2009). In that study, we treated daily those controls with prevalent vertebral fractures with 1 μg alfacalcidol + 500 mg calcium (group A) and those without prevalent vertebral fractures with 1,000 IU plain vitamin D (Vit. D) + 1,000 mg calcium (group B). Subsequently, we added an additional 56 pairs of patients to these two groups: 28 with and 28 without prevalent vertebral fractures, reaching a total of 214 cases. That means with this design, we are comparing two groups with a different risk at onset. Due to the prevalent vertebral fractures and lower average bone mineral density (BMD) values, there was a higher risk of incident fractures in group A. After 2 years, we found significantly higher increases in lumbar spine BMD (+3.2 vs. +0.8 %) and total hip BMD (+1.9 vs. -0.9 %) in group A and B, respectively. Eighteen incident falls were recorded in the alfacalcidol group and 38 in the group treated with Vit. D (p = 0.041). There were significantly lower rates of patients with new vertebral and non-vertebral fractures in group A than in group B. Back pain was significantly reduced only with alfacalcidol. Concerning the incidence of new non-vertebral fractures, we found that there was a relation to renal function in the two groups. The advantage for alfacalcidol was mainly due to a higher non-vertebral fracture-reducing potency in patients with a creatinine clearance (CrCl) below 60 ml/min (p = 0.0019). There

  6. 内镜诊治上消化道异物214例效果研究%Diagnosis and treatment of 214 cases of upper gastrointestinal foreign bodies with endoscopy

    Institute of Scientific and Technical Information of China (English)

    吴小琴; 杨涛; 刘翔; 杨辉; 吕建忠; 丁元伟; 林漫鹏

    2014-01-01

    目的 探讨内镜诊断及处理上消化道异物的方法.方法 回顾性分析我院2006年1月至2013年8月期间共214例吞入异物的患者,进行紧急内镜检查和应用辅助器械配合内镜处理异物的情况.结果 214例患者中异物位于食管内203例、胃内7例、十二指肠4例.共有204例异物经内镜成功取出,成功率为95.3%,未出现严重并发症;尚有10例异物,因异物尖锐端严重嵌顿等原因未取出.结论 应用内镜取出上消化道异物是安全、有效的.

  7. Marker-Assisted Backcrossing to Introgress Resistance to Fusarium Wilt Race 1 and Ascochyta Blight in C 214, an Elite Cultivar of Chickpea

    Directory of Open Access Journals (Sweden)

    R. K. Varshney

    2014-03-01

    Full Text Available Fusarium wilt (FW and Ascochyta blight (AB are two major constraints to chickpea (Cicer arietinum production,Fusarium wilt (FW and Ascochyta blight (AB are two major constraints to chickpea (Cicer arietinum L. production. Therefore, two parallel marker-assisted backcrossing (MABC programs by targeting foc1 locus and two quantitative trait loci (QTL regions, ABQTL-I and ABQTL-II, were undertaken to introgress resistance to FW and AB, respectively, in C 214, an elite cultivar of chickpea. In the case of FW, foreground selection (FGS was conducted with six markers (TR19, TA194, TAA60, GA16, TA110, and TS82 linked to foc1 in the cross C 214 × WR 315 (FW-resistant. On the other hand, eight markers (TA194, TR58, TS82, GA16, SCY17, TA130, TA2, and GAA47 linked with ABQTL-I and ABQTL-II were used in the case of AB by deploying C 214 × ILC 3279 (AB-resistant cross. Background selection (BGS in both crosses was employed with evenly distributed 40 (C 214 × WR 315 to 43 (C 214 × ILC 3279 SSR markers in the chickpea genome to select plant(s with higher recurrent parent genome (RPG recovery. By using three backcrosses and three rounds of selfing, 22 BC3F4 lines were generated for C 214 × WR 315 cross and 14 MABC lines for C 214 × ILC 3279 cross. Phenotyping of these lines has identified three resistant lines (with 92.7–95.2% RPG to race 1 of FW, and seven resistant lines (with 81.7–85.40% RPG to AB that may be tested for yield and other agronomic traits under multilocation trials for possible release and cultivation.

  8. Reconsidering the Resources Needed for Multiple Casualty Events: Lessons Learned From the Crash of Asiana Airlines Flight 214.

    Science.gov (United States)

    Campion, Eric M; Juillard, Catherine; Knudson, M Margaret; Dicker, Rochelle; Cohen, Mitchell J; Mackersie, Robert; Campbell, Andre R; Callcut, Rachael A

    2016-06-01

    To date, a substantial portion of multiple casualty incident literature has focused exclusively on prehospital and emergency department resources needed for optimal disaster response. Thus, inpatient resources required to care for individuals injured in multiple casualty events are not well described. To highlight the resources beyond initial emergency department triage needed for multiple casualty events, using one of the largest commercial aviation disasters in modern US history as a case study. Prospective case series of injured individuals treated at an urban level I trauma center following the crash of Asiana Airlines flight 214 on July 6, 2013. This analysis was conducted between June 1, 2014, and December 1, 2015. Commercial jetliner crash. Medical records, imaging data, nursing overtime, blood bank records, and trauma registry data were analyzed. Disaster logs, patient injuries, and blood product data were prospectively collected during the incident. Among 307 people aboard the flight, 192 were injured; 63 of the injured patients were initially evaluated at San Francisco General Hospital and Trauma Center (the highest number at any of the receiving medical facilities; age range, 4-74 years [23 were aged 60 years]; median injury severity score of 19 admitted patients, 9 [range, 9-45]), including the highest number of critically injured patients (10 of 12). Despite the high impact of the crash, only 3 persons (Airlines flight 214 crash highlights the need to plan for high use of advanced imaging, blood products, operating room availability, nursing resources, and management of inpatient hospital beds.

  9. MiR-214 targets β-catenin pathway to suppress invasion, stem-like traits and recurrence of human hepatocellular carcinoma.

    Directory of Open Access Journals (Sweden)

    Hongping Xia

    Full Text Available The down-regulation of miR-214 has previously been observed in human hepatocellular carcinoma (HCC. Here, we demonstrated the down-regulation of miR-214 is associated with cell invasion, stem-like traits and early recurrence of HCC. Firstly, we validated the suppression of miR-214 in human HCC by real-time quantitative RT-PCR (qRT-PCR in 20 paired tumor and non-tumor liver tissues of HCC patients and 10 histologically normal liver tissues from colorectal cancer patients with liver metastases. Further qRT-PCR analysis of 50 HCC tissues from an independent cohort of HCC patients of whom 29 with early recurrent disease (<2 years and 21 with late recurrent disease demonstrated that the suppression of miR-214 was significantly more suppressed in samples from HCC patients with early recurrent disease compared those from patients with no recurrence. Re-expression of miR-214 significantly suppressed the growth of HCC cells in vitro and reduced their tumorigenicity in vivo. The enhancer of zeste homologue 2 (EZH2 and β-catenin (CTNNB1 was identified as two potential direct downstream targets of miR-214 through bioinformatics analysis and experimentally validated the miRNA-target interactions with a dual-firefly luciferase reporter assay. In corroborate with this, both EZH2 and CTNNB1 are found to be significantly overexpressed in human HCC biopsies. Since EZH2 can regulate CTNNB1, CTNNB1 can also be an indirect target of miR-214 through EZH2. Silencing EZH2 or CTNNB1 expression suppressed the growth and invasion of HCC cells and induced E-cadherin (CDH1, known to inhibit cell invasion and metastasis. Furthermore, the silencing of miR-214 or overexpression of EZH2 increased EpCAM(+ stem-like cells through the activation of CTNNB1. Interestingly, the up-regulation of EZH2, CTNNB1 and the down-regulation of CDH1 in HCC patients correlated with early recurrent disease and can be an independent predictor of poor survival. Therefore, miR-214 can directly or

  10. Production and assay of cellulolytic enzyme activity of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya abbatoir, Indonesia

    Directory of Open Access Journals (Sweden)

    W. P. Lokapirnasari

    2015-03-01

    Full Text Available Aim: This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4-β-D-glucanase, exo-(1,4-β-Dglucanase, and β-glucosidase, at optimum temperature and optimum pH of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia. Materials and Methods: To produce enzyme from a single colony of E. cloacae WPL 214, 98 × 1010 CFU/ml of isolates was put into 20 ml of liquid medium and incubated in a shaker incubator for 16 h at 35°C in accordance with growth time and optimum temperature of E. cloacae WPL 214. Further on, culture was centrifuged at 6000 rpm at 4°C for 15 min. Pellet was discarded while supernatant containing cellulose enzyme activity was withdrawn to assay endo-(1,4-β-D-glucanase, exo-(1,4-β-D-glucanase, and β-glucosidase. Results: Cellulase enzyme of E. cloacae WPL 214 isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of 0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35°C and optimum pH 5. Conclusion: E. cloacae WPL 214 isolated from bovine rumen fluid waste produced cellulose enzyme with activity as cellulolytic enzyme of endo-(1,4-β-D-glucanase, exo-(1,4-β-D-glucanase and β-glucosidase.

  11. Luminosity determination for the pd reaction at 2.14 GeV with WASA-at-COSY

    Institute of Scientific and Technical Information of China (English)

    ZHENG Chuan; M. Büscher; P. Fedorets; V.Hejny; H. Str(o)her; XU Hu-Shan; YUAN Xiao-Hua

    2012-01-01

    The luminosity for a WASA-at-COSY experiment involving the pd reaction at 2.14 GeV protonbeam energy is determined by the forward pd elastic scattering,which yields an average beam-on-target value of [5.2±0.3(stat)±0.3(syst)] × 1030s-1 cm-2.In addition,the forward pd elastic-scattering angular distribution is obtained with four-momentum transfer squared -t between 0.16 (GeV/c)2 and 0.78 (GeV/c)2 at this beam energy,which is compared with other experimental data and the pd double scattering model.

  12. Molecular glass positive i-line photoresist materials containing 2,1,4-DNQ and acid labile group

    Science.gov (United States)

    Wang, Liyuan; Yu, Jinxing; Xu, Na

    2010-04-01

    Recent years increasing attention has been given to molecular glass resist materials. In this paper, maleopimaric acid, cycloaddition reaction product of rosin with maleic anhydride, was reacted with hydroxylamine and then further esterified with 2-diazo-1-naphthoquinone-4-sulfonyl chloride to give N-hydroxy maleopimarimide sulfonate. The carboxylic acid group of the compound was then protected by the reaction of this compound with vinyl ethyl ether or dihydropyran. Thus obtained compounds were amorphous. When irradiated with i-line light, the 2,1,4-DNQ group undergo photolysis not only to give off nitrogen gas but also generate sulfonic acid which can result in the decomposition of the acid labile group. So, a novel chemically amplified positive i-line molecular glass photoresists can be formed by the compound and other acidolytic molecular glass compounds. The lithographic performance of the resist materials is evaluated.

  13. Degradation of Triazine-2-(14C Metsulfuron-Methyl in Soil from an Oil Palm Plantation.

    Directory of Open Access Journals (Sweden)

    B S Ismail

    Full Text Available Triazine-2-(14C metsulfuron-methyl is a selective, systemic sulfonylurea herbicide. Degradation studies in soils are essential for the evaluation of the persistence of pesticides and their breakdown products. The purpose of the present study was to investigate the degradation of triazine-2-(14C metsulfuron-methyl in soil under laboratory conditions. A High Performance Liquid Chromatograph (HPLC equipped with an UV detector and an on-line radio-chemical detector, plus a Supelco Discovery column (250 x 4.6 mm, 5 μm, and PRP-1 column (305 x 7.0 mm, 10 μm was used for the HPLC analysis. The radioactivity was determined by a Liquid Scintillation Counter (LSC in scintillation fluid. The soil used was both sterilized and non-sterilized in order to observe the involvement of soil microbes. The estimated DT50 and DT90 values of metsulfuron-methyl in a non-sterile system were observed to be 13 and 44 days, whereas in sterilized soil, the DT50 and DT90 were 31 and 70 days, respectively. The principal degradation product after 60 days was CO2. The higher cumulative amount of (14CO2 in (14C-triazine in the non-sterilized soil compared to that in the sterile system suggests that biological degradation by soil micro-organisms significantly contributes to the dissipation of the compound. The major routes of degradation were O-demethylation, sulfonylurea bridge cleavage and the triazine "ring-opened."

  14. Degradation of Triazine-2-14C Metsulfuron–Methyl in Soil from an Oil Palm Plantation

    Science.gov (United States)

    B. S., Ismail

    2015-01-01

    Triazine-2-14C metsulfuron–methyl is a selective, systemic sulfonylurea herbicide. Degradation studies in soils are essential for the evaluation of the persistence of pesticides and their breakdown products. The purpose of the present study was to investigate the degradation of triazine-2-14C metsulfuron–methyl in soil under laboratory conditions. A High Performance Liquid Chromatograph (HPLC) equipped with an UV detector and an on-line radio-chemical detector, plus a Supelco Discovery column (250 x 4.6 mm, 5 μm), and PRP–1 column (305 x 7.0 mm, 10 μm) was used for the HPLC analysis. The radioactivity was determined by a Liquid Scintillation Counter (LSC) in scintillation fluid. The soil used was both sterilized and non-sterilized in order to observe the involvement of soil microbes. The estimated DT50 and DT90 values of metsulfuron-methyl in a non-sterile system were observed to be 13 and 44 days, whereas in sterilized soil, the DT50 and DT90 were 31 and 70 days, respectively. The principal degradation product after 60 days was CO2. The higher cumulative amount of 14CO2 in 14C- triazine in the non-sterilized soil compared to that in the sterile system suggests that biological degradation by soil micro-organisms significantly contributes to the dissipation of the compound. The major routes of degradation were O-demethylation, sulfonylurea bridge cleavage and the triazine “ring-opened.” PMID:26437264

  15. Expression of SET-NUP214 Fusion Gene in Patients with T-cell Acute Lymphoblastic Leukemia and Its Clinical Significance%SET-NUP214融合基因在急性T淋巴细胞白血病患者中的表达及临床意义

    Institute of Scientific and Technical Information of China (English)

    戴海萍; 陈苏宁; 王谦; 吴丽丽; 平娜娜; 吴春晓; 解珺丹; 潘金兰; 薛永权; 吴德沛

    2012-01-01

    本研究旨在探讨SET-NUP214融合基因在急性T淋巴细胞白血病(T-ALL)中的表达,分析伴有SET-NUP214的T-ALL的临床及分子生物学特征.运用RT-PCR检测58例T-ALL患者骨髓标本中SET-NUP214的表达,以间期荧光原位杂交( FISH)及微阵列比较基因组杂交(Array-CGH)检测9q34缺失,基因测序法检测PHF6和NOTCH1基因突变.结果表明,仅在6例T-ALL患者中检测到SET-NUP214融合基因的表达.除T系抗原标记外,这些患者均表达髓系抗原CD13和(或)CD33,其中4例患者经FISH检测到9q34缺失,3例患者经Array-CGH检测到del(9)( q34.11q34.33).6例SET-NUP214阳性的T-ALL患者中有4例检测到PHF6基因突变,5例检测到NOTCH1基因突变.结论:SET-NUP214融合基因多由染色体9q34的缺失所致,SET-NUP214阳性的T-ALL常伴有PHF6及NOTCH基因突变.%This study was aimed to investigate the occurrence and clinical significance of the SET-NUP214 fusion gene in patients with T-cell acute lymphoblastic leukemia (T-ALL) , analyse clinical and biological characteristics in this disease. RT-PCR was used to detect the expression of SET-NUP214 fusion gene in 58 T-ALL cases. Interphase FISH and Array-CGH were used to detect the deletion of 9q34. Direct sequencing was applied to detect mutations of PHF6 and NOTCH1. The results showed that 6 out of 58 T-ALL cases (10. 3% ) were detected to have the SET-NUP214 fusion gene by RT-PCR. Besides T-lineage antigens, expression of CD13 and(or) CD33 were detected in all the 6 cases. Deletions of 9q34 were detected in 4 out of the 6 patients by FISH. Array-CGH results of 3 SET-NUP214 positive T-ALL patients confirmed that this fusion gene was resulted from a cryptic deletion of 9q34. Ilq34. 13. PHF6 and N0TCH1 gene mutations were found in 4 and 5 out of 6 SET-NUP214 positive T-ALL patients, respectively. It is concluded that SET-NUP214 fusion gene is often resulted from del(9) (q34). PHF6 and N0TCH1 mutations may be potential leukemo-genic event in SET

  16. Contribution of plated-out 218Po and 214Po to measurements of airborne 222Rn and daughters with plastic (CR-39) nuclear track detectors

    Science.gov (United States)

    Kahn, Bernd; Wang, Zuoyuan; Sensistaffar, Edwin

    1984-01-01

    The fraction of alpha-particle tracks due to radioactivity plated out on its surface was measured for CR-39 nuclear track detector foils used to determine working level values in air. Bare foils were exposed to known concentrations of airborne 222Rn and its short-lived daughters in a calibration chamber. The amounts of 218Po and 214Po on the foil surface were measured with a calibrated diffused junction detector-spectrometer system immediately after the foils were removed from the chamber. Deposition was mostly by 218Po, with some 214Pb but essentially no 214Bi. The track density due to the plated-out radionuclides and the 222Rn, 218Po, and 214Po in chamber air was calculated and compared to the value measured by electrochemical etching. The calculated values generally were slightly above the measured values. On the basis of these calculations, the deposited radioactivity contributed slightly less than one-half of the total tracks in one test and slightly more than two-thirds in another. This effect complicates calibration of the detector relative to airborne radon daughters.

  17. Planck intermediate results : VI. The dynamical structure of PLCKG214.6+37.0, a Planck discovered triple system of galaxy clusters

    NARCIS (Netherlands)

    Ade, P. A. R.; Aghanim, N.; Arnaud, M.; Ashdown, M.; Atrio-Barandela, F.; Aumont, J.; Baccigalupi, C.; Balbi, A.; Banday, A. J.; Barreiro, R. B.; Bartlett, J. G.; Battaner, E.; Benabed, K.; Benoit, A.; Bernard, J. -P.; Bersanelli, M.; Bhatia, R.; Bikmaev, I.; Boehringer, H.; Bonaldi, A.; Bond, J. R.; Borrill, J.; Bouchet, F. R.; Bourdin, H.; Burenin, R.; Burigana, C.; Cabella, P.; Cardoso, J.-F.; Castex, G.; Catalano, A.; Cayon, L.; Chamballu, A.; Chiang, L. -Y; Chon, G.; Christensen, P. R.; Clements, D. L.; Colafrancesco, S.; Colombi, S.; Colombo, L. P. L.; Comis, B.; Coulais, A.; Crill, B. P.; Cuttaia, F.; Da Silva, A.; Dahle, H.; Danese, L.; Davis, R. J.; de Bernardis, P.; de Gasperis, G.; de Zotti, G.; Delabrouille, J.; Democles, J.; Diego, J. M.; Dolag, K.; Dole, H.; Donzelli, S.; Dore, O.; Doerl, U.; Douspis, M.; Dupac, X.; Efstathiou, G.; Ensslin, T. A.; Eriksen, H. K.; Finelli, F.; Flores-Cacho, I.; Forni, O.; Frailis, M.; Franceschi, E.; Frommert, M.; Galeotta, S.; Ganga, K.; Genova-Santos, R. T.; Giard, M.; Gilfanov, M.; Giraud-Heraud, Y.; Gonzalez-Nuevo, J.; Gorski, K. M.; Gregorio, A.; Gruppuso, A.; Hansen, F. K.; Harrison, D.; Heinamaki, P.; Hempel, A.; Henrot-Versille, S.; Hernandez-Monteagudo, C.; Herranz, D.; Hildebrandt, S. R.; Hivon, E.; Hobson, M.; Holmes, W. A.; Hurier, G.; Jaffe, T. R.; Jaffe, A. H.; Jagemann, T.; Jones, W. C.; Juvela, M.; Keihaenen, E.; Khamitov, I.; Kisner, T. S.; Kneissl, R.; Knoche, J.; Knox, L.; Kunz, M.; Kurki-Suonio, H.; Lagache, G.; Lahteenmaki, A.; Lamarre, J. -M.; Lasenby, A.; Lawrence, C. R.; Le Jeune, M.; Leonardi, R.; Lilje, P. B.; Lopez-Caniego, M.; Luzzi, G.; Macias-Perez, J. F.; Maino, D.; Mandolesi, N.; Maris, M.; Marleau, F.; Marshall, D. J.; Martinez-Gonzalez, E.; Masi, S.; Massardi, M.; Matarrese, S.; Mazzotta, P.; Mei, S.; Melchiorri, A.; Melin, J. -B.; Mendes, L.; Mennella, A.; Mitra, S.; Miville-Deschenes, M. -A.; Moneti, A.; Montier, L.; Morgante, G.; Mortlock, D.; Munshi, D.; Murphy, J. A.; Naselsky, P.; Nati, F.; Natoli, P.; Norgaard-Nielsen, H. U.; Noviello, F.; Novikov, D.; Novikov, I.; Osborne, S.; Pajot, F.; Paoletti, D.; Pasian, F.; Patanchon, G.; Perdereau, O.; Perotto, L.; Perrotta, F.; Piacentini, F.; Piat, M.; Pierpaoli, E.; Piffaretti, R.; Plaszczynski, S.; Pointecouteau, E.; Polenta, G.; Ponthieu, N.; Popa, L.; Poutanen, T.; Pratt, G. W.; Prunet, S.; Puget, J. -L.; Rachen, J. P.; Rebolo, R.; Reinecke, M.; Remazeilles, M.; Renault, C.; Ricciardi, S.; Riller, T.; Ristorcelli, I.; Rocha, G.; Roman, M.; Rosset, C.; Rossetti, M.; Rubino-Martin, J. A.; Rusholme, B.; Sandri, M.; Savini, G.; Scott, D.; Smoot, G. F.; Starck, J. -L.; Sudiwala, R.; Sunyaev, R.; Sutton, D.; Suur-Uski, A. -S.; Sygnet, J. -F.; Tauber, J. A.; Terenzi, L.; Toffolatti, L.; Tomasi, M.; Tristram, M.; Tuovinen, J.; Valenziano, L.; Van Tent, B.; Vielva, P.; Villa, F.; Vittorio, N.; Wade, L. A.; Wandelt, B. D.; Welikala, N.; Yvon, D.; Zacchei, A.; Zaroubi, S.; Zonca, A.

    2013-01-01

    The survey of galaxy clusters performed by Planck through the Sunyaev-Zeldovich effect has already discovered many interesting objects, thanks to its full sky coverage. One of the SZ candidates detected in the early months of the mission near to the signal-to-noise threshold, PLCKG214.6+37.0, was la

  18. Planck intermediate results: VI. The dynamical structure of PLCKG214.6+37.0, a Planck discovered triple system of galaxy clusters

    DEFF Research Database (Denmark)

    Castex, G.; Delabrouille, J.; Ganga, K.

    2013-01-01

    .6+37.0, was later revealed by XMM-Newton to be a triple system of galaxy clusters. We present the results from a deep XMM-Newton re-observation of PLCKG214.6+37.0, part of a multi-wavelength programme to investigate Planck discovered superclusters. The characterisation of the physical properties of the three...

  19. Planck intermediate results. VI. The dynamical structure of PLCKG214.6+37.0, a Planck discovered triple system of galaxy clusters

    NARCIS (Netherlands)

    Planck Collaboration, [Unknown; Ade, P. A. R.; Aghanim, N.; Arnaud, M.; Ashdown, M.; Atrio-Barandela, F.; Aumont, J.; Baccigalupi, C.; Balbi, A.; Banday, A. J.; Barreiro, R. B.; Bartlett, J. G.; Battaner, E.; Benabed, K.; Benoît, A.; Bernard, J.-P.; Bersanelli, M.; Bhatia, R.; Bikmaev, I.; Böhringer, H.; Bonaldi, A.; Bond, J. R.; Borrill, J.; Bouchet, F. R.; Bourdin, H.; Burenin, R.; Burigana, C.; Cabella, P.; Cardoso, J.-F.; Castex, G.; Catalano, A.; Cayón, L.; Chamballu, A.; Chiang, L.-Y.; Chon, G.; Christensen, P. R.; Clements, D. L.; Colafrancesco, S.; Colombi, S.; Colombo, L. P. L.; Comis, B.; Coulais, A.; Crill, B. P.; Cuttaia, F.; Da Silva, A.; Dahle, H.; Danese, L.; Davis, R. J.; de Bernardis, P.; de Gasperis, G.; de Zotti, G.; Delabrouille, J.; Démoclès, J.; Diego, J. M.; Dolag, K.; Dole, H.; Donzelli, S.; Doré, O.; Dörl, U.; Douspis, M.; Dupac, X.; Efstathiou, G.; Enßlin, T. A.; Eriksen, H. K.; Finelli, F.; Flores-Cacho, I.; Forni, O.; Frailis, M.; Franceschi, E.; Frommert, M.; Galeotta, S.; Ganga, K.; Génova-Santos, R. T.; Giard, M.; Gilfanov, M.; Giraud-Héraud, Y.; González-Nuevo, J.; Górski, K. M.; Gregorio, A.; Gruppuso, A.; Hansen, F. K.; Harrison, D.; Heinämäki, P.; Hempel, A.; Henrot-Versillé, S.; Hernández-Monteagudo, C.; Herranz, D.; Hildebrandt, S. R.; Hivon, E.; Hobson, M.; Holmes, W. A.; Hurier, G.; Jaffe, T. R.; Jaffe, A. H.; Jagemann, T.; Jones, W. C.; Juvela, M.; Keihänen, E.; Khamitov, I.; Kisner, T. S.; Kneissl, R.; Knoche, J.; Knox, L.; Kunz, M.; Kurki-Suonio, H.; Lagache, G.; Lähteenmäki, A.; Lamarre, J.-M.; Lasenby, A.; Lawrence, C. R.; Le Jeune, M.; Leonardi, R.; Lilje, P. B.; López-Caniego, M.; Luzzi, G.; Macías-Pérez, J. F.; Maino, D.; Mandolesi, N.; Maris, M.; Marleau, F.; Marshall, D. J.; Martínez-González, E.; Masi, S.; Massardi, M.; Matarrese, S.; Mazzotta, P.; Mei, S.; Melchiorri, A.; Melin, J.-B.; Mendes, L.; Mennella, A.; Mitra, S.; Miville-Deschênes, M.-A.; Moneti, A.; Montier, L.; Morgante, G.; Mortlock, D.; Munshi, D.; Murphy, J. A.; Naselsky, P.; Nati, F.; Natoli, P.; Nørgaard-Nielsen, H. U.; Noviello, F.; Novikov, D.; Novikov, I.; Osborne, S.; Pajot, F.; Paoletti, D.; Pasian, F.; Patanchon, G.; Perdereau, O.; Perotto, L.; Perrotta, F.; Piacentini, F.; Piat, M.; Pierpaoli, E.; Piffaretti, R.; Plaszczynski, S.; Pointecouteau, E.; Polenta, G.; Ponthieu, N.; Popa, L.; Poutanen, T.; Pratt, G. W.; Prunet, S.; Puget, J.-L.; Rachen, J. P.; Rebolo, R.; Reinecke, M.; Remazeilles, M.; Renault, C.; Ricciardi, S.; Riller, T.; Ristorcelli, I.; Rocha, G.; Roman, M.; Rosset, C.; Rossetti, M.; Rubiño-Martín, J. A.; Rusholme, B.; Sandri, M.; Savini, G.; Scott, D.; Smoot, G. F.; Starck, J.-L.; Sudiwala, R.; Sunyaev, R.; Sutton, D.; Suur-Uski, A.-S.; Sygnet, J.-F.; Tauber, J. A.; Terenzi, L.; Toffolatti, L.; Tomasi, M.; Tristram, M.; Tuovinen, J.; Valenziano, L.; Van Tent, B.; Vielva, P.; Villa, F.; Vittorio, N.; Wade, L. A.; Wandelt, B. D.; Welikala, N.; Yvon, D.; Zacchei, A.; Zaroubi, S.; Zonca, A.

    2013-01-01

    The survey of galaxy clusters performed by Planck through the Sunyaev-Zeldovich effect has already discovered many interesting objects, thanks to its full sky coverage. One of the SZ candidates detected inthe early months of the mission near to the signal-to-noise threshold, PLCKG214.6+37.0, was lat

  20. Planck intermediate results : VI. The dynamical structure of PLCKG214.6+37.0, a Planck discovered triple system of galaxy clusters

    NARCIS (Netherlands)

    Ade, P. A. R.; Aghanim, N.; Arnaud, M.; Ashdown, M.; Atrio-Barandela, F.; Aumont, J.; Baccigalupi, C.; Balbi, A.; Banday, A. J.; Barreiro, R. B.; Bartlett, J. G.; Battaner, E.; Benabed, K.; Benoit, A.; Bernard, J. -P.; Bersanelli, M.; Bhatia, R.; Bikmaev, I.; Boehringer, H.; Bonaldi, A.; Bond, J. R.; Borrill, J.; Bouchet, F. R.; Bourdin, H.; Burenin, R.; Burigana, C.; Cabella, P.; Cardoso, J.-F.; Castex, G.; Catalano, A.; Cayon, L.; Chamballu, A.; Chiang, L. -Y; Chon, G.; Christensen, P. R.; Clements, D. L.; Colafrancesco, S.; Colombi, S.; Colombo, L. P. L.; Comis, B.; Coulais, A.; Crill, B. P.; Cuttaia, F.; Da Silva, A.; Dahle, H.; Danese, L.; Davis, R. J.; de Bernardis, P.; de Gasperis, G.; de Zotti, G.; Delabrouille, J.; Democles, J.; Diego, J. M.; Dolag, K.; Dole, H.; Donzelli, S.; Dore, O.; Doerl, U.; Douspis, M.; Dupac, X.; Efstathiou, G.; Ensslin, T. A.; Eriksen, H. K.; Finelli, F.; Flores-Cacho, I.; Forni, O.; Frailis, M.; Franceschi, E.; Frommert, M.; Galeotta, S.; Ganga, K.; Genova-Santos, R. T.; Giard, M.; Gilfanov, M.; Giraud-Heraud, Y.; Gonzalez-Nuevo, J.; Gorski, K. M.; Gregorio, A.; Gruppuso, A.; Hansen, F. K.; Harrison, D.; Heinamaki, P.; Hempel, A.; Henrot-Versille, S.; Hernandez-Monteagudo, C.; Herranz, D.; Hildebrandt, S. R.; Hivon, E.; Hobson, M.; Holmes, W. A.; Hurier, G.; Jaffe, T. R.; Jaffe, A. H.; Jagemann, T.; Jones, W. C.; Juvela, M.; Keihaenen, E.; Khamitov, I.; Kisner, T. S.; Kneissl, R.; Knoche, J.; Knox, L.; Kunz, M.; Kurki-Suonio, H.; Lagache, G.; Lahteenmaki, A.; Lamarre, J. -M.; Lasenby, A.; Lawrence, C. R.; Le Jeune, M.; Leonardi, R.; Lilje, P. B.; Lopez-Caniego, M.; Luzzi, G.; Macias-Perez, J. F.; Maino, D.; Mandolesi, N.; Maris, M.; Marleau, F.; Marshall, D. J.; Martinez-Gonzalez, E.; Masi, S.; Massardi, M.; Matarrese, S.; Mazzotta, P.; Mei, S.; Melchiorri, A.; Melin, J. -B.; Mendes, L.; Mennella, A.; Mitra, S.; Miville-Deschenes, M. -A.; Moneti, A.; Montier, L.; Morgante, G.; Mortlock, D.; Munshi, D.; Murphy, J. A.; Naselsky, P.; Nati, F.; Natoli, P.; Norgaard-Nielsen, H. U.; Noviello, F.; Novikov, D.; Novikov, I.; Osborne, S.; Pajot, F.; Paoletti, D.; Pasian, F.; Patanchon, G.; Perdereau, O.; Perotto, L.; Perrotta, F.; Piacentini, F.; Piat, M.; Pierpaoli, E.; Piffaretti, R.; Plaszczynski, S.; Pointecouteau, E.; Polenta, G.; Ponthieu, N.; Popa, L.; Poutanen, T.; Pratt, G. W.; Prunet, S.; Puget, J. -L.; Rachen, J. P.; Rebolo, R.; Reinecke, M.; Remazeilles, M.; Renault, C.; Ricciardi, S.; Riller, T.; Ristorcelli, I.; Rocha, G.; Roman, M.; Rosset, C.; Rossetti, M.; Rubino-Martin, J. A.; Rusholme, B.; Sandri, M.; Savini, G.; Scott, D.; Smoot, G. F.; Starck, J. -L.; Sudiwala, R.; Sunyaev, R.; Sutton, D.; Suur-Uski, A. -S.; Sygnet, J. -F.; Tauber, J. A.; Terenzi, L.; Toffolatti, L.; Tomasi, M.; Tristram, M.; Tuovinen, J.; Valenziano, L.; Van Tent, B.; Vielva, P.; Villa, F.; Vittorio, N.; Wade, L. A.; Wandelt, B. D.; Welikala, N.; Yvon, D.; Zacchei, A.; Zaroubi, S.; Zonca, A.

    2013-01-01

    The survey of galaxy clusters performed by Planck through the Sunyaev-Zeldovich effect has already discovered many interesting objects, thanks to its full sky coverage. One of the SZ candidates detected in the early months of the mission near to the signal-to-noise threshold, PLCKG214.6+37.0, was la

  1. Planck intermediate results. VI. The dynamical structure of PLCKG214.6+37.0, a Planck discovered triple system of galaxy clusters

    NARCIS (Netherlands)

    Planck Collaboration, [Unknown; Ade, P. A. R.; Aghanim, N.; Arnaud, M.; Ashdown, M.; Atrio-Barandela, F.; Aumont, J.; Baccigalupi, C.; Balbi, A.; Banday, A. J.; Barreiro, R. B.; Bartlett, J. G.; Battaner, E.; Benabed, K.; Benoît, A.; Bernard, J.-P.; Bersanelli, M.; Bhatia, R.; Bikmaev, I.; Böhringer, H.; Bonaldi, A.; Bond, J. R.; Borrill, J.; Bouchet, F. R.; Bourdin, H.; Burenin, R.; Burigana, C.; Cabella, P.; Cardoso, J.-F.; Castex, G.; Catalano, A.; Cayón, L.; Chamballu, A.; Chiang, L.-Y.; Chon, G.; Christensen, P. R.; Clements, D. L.; Colafrancesco, S.; Colombi, S.; Colombo, L. P. L.; Comis, B.; Coulais, A.; Crill, B. P.; Cuttaia, F.; Da Silva, A.; Dahle, H.; Danese, L.; Davis, R. J.; de Bernardis, P.; de Gasperis, G.; de Zotti, G.; Delabrouille, J.; Démoclès, J.; Diego, J. M.; Dolag, K.; Dole, H.; Donzelli, S.; Doré, O.; Dörl, U.; Douspis, M.; Dupac, X.; Efstathiou, G.; Enßlin, T. A.; Eriksen, H. K.; Finelli, F.; Flores-Cacho, I.; Forni, O.; Frailis, M.; Franceschi, E.; Frommert, M.; Galeotta, S.; Ganga, K.; Génova-Santos, R. T.; Giard, M.; Gilfanov, M.; Giraud-Héraud, Y.; González-Nuevo, J.; Górski, K. M.; Gregorio, A.; Gruppuso, A.; Hansen, F. K.; Harrison, D.; Heinämäki, P.; Hempel, A.; Henrot-Versillé, S.; Hernández-Monteagudo, C.; Herranz, D.; Hildebrandt, S. R.; Hivon, E.; Hobson, M.; Holmes, W. A.; Hurier, G.; Jaffe, T. R.; Jaffe, A. H.; Jagemann, T.; Jones, W. C.; Juvela, M.; Keihänen, E.; Khamitov, I.; Kisner, T. S.; Kneissl, R.; Knoche, J.; Knox, L.; Kunz, M.; Kurki-Suonio, H.; Lagache, G.; Lähteenmäki, A.; Lamarre, J.-M.; Lasenby, A.; Lawrence, C. R.; Le Jeune, M.; Leonardi, R.; Lilje, P. B.; López-Caniego, M.; Luzzi, G.; Macías-Pérez, J. F.; Maino, D.; Mandolesi, N.; Maris, M.; Marleau, F.; Marshall, D. J.; Martínez-González, E.; Masi, S.; Massardi, M.; Matarrese, S.; Mazzotta, P.; Mei, S.; Melchiorri, A.; Melin, J.-B.; Mendes, L.; Mennella, A.; Mitra, S.; Miville-Deschênes, M.-A.; Moneti, A.; Montier, L.; Morgante, G.; Mortlock, D.; Munshi, D.; Murphy, J. A.; Naselsky, P.; Nati, F.; Natoli, P.; Nørgaard-Nielsen, H. U.; Noviello, F.; Novikov, D.; Novikov, I.; Osborne, S.; Pajot, F.; Paoletti, D.; Pasian, F.; Patanchon, G.; Perdereau, O.; Perotto, L.; Perrotta, F.; Piacentini, F.; Piat, M.; Pierpaoli, E.; Piffaretti, R.; Plaszczynski, S.; Pointecouteau, E.; Polenta, G.; Ponthieu, N.; Popa, L.; Poutanen, T.; Pratt, G. W.; Prunet, S.; Puget, J.-L.; Rachen, J. P.; Rebolo, R.; Reinecke, M.; Remazeilles, M.; Renault, C.; Ricciardi, S.; Riller, T.; Ristorcelli, I.; Rocha, G.; Roman, M.; Rosset, C.; Rossetti, M.; Rubiño-Martín, J. A.; Rusholme, B.; Sandri, M.; Savini, G.; Scott, D.; Smoot, G. F.; Starck, J.-L.; Sudiwala, R.; Sunyaev, R.; Sutton, D.; Suur-Uski, A.-S.; Sygnet, J.-F.; Tauber, J. A.; Terenzi, L.; Toffolatti, L.; Tomasi, M.; Tristram, M.; Tuovinen, J.; Valenziano, L.; Van Tent, B.; Vielva, P.; Villa, F.; Vittorio, N.; Wade, L. A.; Wandelt, B. D.; Welikala, N.; Yvon, D.; Zacchei, A.; Zaroubi, S.; Zonca, A.

    2013-01-01

    The survey of galaxy clusters performed by Planck through the Sunyaev-Zeldovich effect has already discovered many interesting objects, thanks to its full sky coverage. One of the SZ candidates detected inthe early months of the mission near to the signal-to-noise threshold, PLCKG214.6+37.0, was lat

  2. The recurrent SET-NUP214 fusion as a new HOXA activation mechanism in pediatric T-cell acute lymphoblastic leukemia.

    Science.gov (United States)

    Van Vlierberghe, Pieter; van Grotel, Martine; Tchinda, Joëlle; Lee, Charles; Beverloo, H Berna; van der Spek, Peter J; Stubbs, Andrew; Cools, Jan; Nagata, Kyosuke; Fornerod, Maarten; Buijs-Gladdines, Jessica; Horstmann, Martin; van Wering, Elisabeth R; Soulier, Jean; Pieters, Rob; Meijerink, Jules P P

    2008-05-01

    T-cell acute lymphoblastic leukemia (T-ALL) is mostly characterized by specific chromosomal abnormalities, some occurring in a mutually exclusive manner that possibly delineate specific T-ALL subgroups. One subgroup, including MLL-rearranged, CALM-AF10 or inv (7)(p15q34) patients, is characterized by elevated expression of HOXA genes. Using a gene expression-based clustering analysis of 67 T-ALL cases with recurrent molecular genetic abnormalities and 25 samples lacking apparent aberrations, we identified 5 new patients with elevated HOXA levels. Using microarray-based comparative genomic hybridization (array-CGH), a cryptic and recurrent deletion, del (9)(q34.11q34.13), was exclusively identified in 3 of these 5 patients. This deletion results in a conserved SET-NUP214 fusion product, which was also identified in the T-ALL cell line LOUCY. SET-NUP214 binds in the promoter regions of specific HOXA genes, where it interacts with CRM1 and DOT1L, which may transcriptionally activate specific members of the HOXA cluster. Targeted inhibition of SET-NUP214 by siRNA abolished expression of HOXA genes, inhibited proliferation, and induced differentiation in LOUCY but not in other T-ALL lines. We conclude that SET-NUP214 may contribute to the pathogenesis of T-ALL by enforcing T-cell differentiation arrest.

  3. Dynamically-driven inactivation of the catalytic machinery of the SARS 3C-like protease by the N214A mutation on the extra domain.

    Science.gov (United States)

    Shi, Jiahai; Han, Nanyu; Lim, Liangzhong; Lua, Shixiong; Sivaraman, J; Wang, Lushan; Mu, Yuguang; Song, Jianxing

    2011-02-01

    Despite utilizing the same chymotrypsin fold to host the catalytic machinery, coronavirus 3C-like proteases (3CLpro) noticeably differ from picornavirus 3C proteases in acquiring an extra helical domain in evolution. Previously, the extra domain was demonstrated to regulate the catalysis of the SARS-CoV 3CLpro by controlling its dimerization. Here, we studied N214A, another mutant with only a doubled dissociation constant but significantly abolished activity. Unexpectedly, N214A still adopts the dimeric structure almost identical to that of the wild-type (WT) enzyme. Thus, we conducted 30-ns molecular dynamics (MD) simulations for N214A, WT, and R298A which we previously characterized to be a monomer with the collapsed catalytic machinery. Remarkably, three proteases display distinctive dynamical behaviors. While in WT, the catalytic machinery stably retains in the activated state; in R298A it remains largely collapsed in the inactivated state, thus implying that two states are not only structurally very distinguishable but also dynamically well separated. Surprisingly, in N214A the catalytic dyad becomes dynamically unstable and many residues constituting the catalytic machinery jump to sample the conformations highly resembling those of R298A. Therefore, the N214A mutation appears to trigger the dramatic change of the enzyme dynamics in the context of the dimeric form which ultimately inactivates the catalytic machinery. The present MD simulations represent the longest reported so far for the SARS-CoV 3CLpro, unveiling that its catalysis is critically dependent on the dynamics, which can be amazingly modulated by the extra domain. Consequently, mediating the dynamics may offer a potential avenue to inhibit the SARS-CoV 3CLpro.

  4. Investigation on drug-resistance of 214 strains of Escherichia coli from various specimens%不同标本大肠埃希菌214株耐药性调查

    Institute of Scientific and Technical Information of China (English)

    赵丽娟; 汤丽霞; 黄干荣; 覃志坚

    2002-01-01

    目的调查不同标本大肠埃希菌(E. Coli)的分布及耐药特点.方法常规方法对临床各种标本进行细菌分离培养,采用FORTUNE2000微生物鉴定仪进行鉴定和药敏试验.结果 214株E. Coli中从尿中分离出96株(44.86%),占各类标本的首位.尿中与非尿中E. Coli的耐药性不同,尿中E. Coli对复合磺胺、氟哌酸、环丙沙星等的耐药率显著高于其他部位分离者(P〈0.05),而尿中E. Coli对呋喃妥因的耐药率明显低于非尿中E. Coli(P<0.05).在22种抗生素中,对5种以上耐药的菌株有162株.结论尿中分离的E. Coli对环丙沙星、氟哌酸、氯霉素、复合磺胺耐药率高于其他标本分离者.

  5. Pigmented villonodular synovitis of the knee: A retrospective analysis of 214 cases at a UK tertiary referral centre.

    Science.gov (United States)

    Patel, K H; Gikas, P D; Pollock, R C; Carrington, R W; Cannon, S R; Skinner, J A; Briggs, T W; Aston, W J S

    2017-08-01

    Pigmented villonodular synovitis (PVNS) is a rare, locally aggressive and potentially recurrent synovial disease. We present the largest single-centre experience of knee PVNS. Our aim was to evaluate our tertiary hospital's experience in the management of knee PVNS. Retrospective data collection of consecutive cases of knee PVNS from 2002 to 2015. In total, 214 cases of knee PVNS were identified which represented 53.4% of all PVNS (12.1% were recurrent at presentation). 100 were localised PVNS (LPVNS), 114 diffuse PVNS (DPVNS) and two malignant PVNS. Knee PVNS was more likely to occur in females with a mean age of 39. Following surgery, 47.6% had recurrence with DPVNS as opposed to 8.6% with LPVNS. In LPVNS, there was no significant difference in recurrence between open and arthroscopic synovectomy (8.7% vs 9.1%, P>0.05). However, in DPVNS, there was a significantly higher risk of recurrence with arthroscopic compared to open synovectomy (83.3% vs 44.8%, RR=1.86 95% CI 1.32-2.62, P=0.0004). PVNS can be difficult to treat. We found no difference in local recurrence rates between open and arthroscopic treatment of LPVNS but significantly increased rates of recurrence for DPVNS following arthroscopic treatment. We would therefore recommend open synovectomy for DPVNS. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  6. SXP214, an X-ray Pulsar in the Small Magellanic Cloud, Crossing the Circumstellar Disk of the Companion

    CERN Document Server

    Hong, JaeSub; Zezas, Andreas; Haberl, Frank; Drake, Jeremy J; Plucinsky, Paul P; Gaetz, Terrance; Sasaki, Manami; Williams, Benjamin; Long, Knox S; Blair, William P; Winkler, P Frank; Wright, Nicholas J; Laycock, Silas; Udalski, Andrzej

    2016-01-01

    Located in the Small Magellanic Cloud (SMC), SXP214 is an X-ray pulsar in a high mass X-ray binary system with a Be-star companion. A recent survey of the SMC under a Chandra X-ray Visionary program found the source in a transition when the X-ray flux was on a steady rise. The Lomb-Scargle periodogram revealed a pulse period of 211.49 +/- 0.42 s, which is significantly (>5sigma) shorter than the previous measurements with XMM-Newton and RXTE. This implies that the system has gone through sudden spin-up episodes recently. The pulse profile shows a sharp eclipse-like feature with a modulation amplitude of >95%. The linear rise of the observed X-ray luminosity from <~2x to 7x10^35 erg s^-1 is correlated with steady softening of the X-ray spectrum, which can be described by the changes in the local absorption from N_H ~ 10^24 to <~10^20 cm^-2 for an absorbed power-law model. The soft X-ray emission below 2 keV was absent in the early part of the observation when only the pulsating hard X-ray component was o...

  7. Physical-mechanical properties and bonding quality of heat treated poplar (I-214 clone and ceiba plywood

    Directory of Open Access Journals (Sweden)

    Goli G

    2015-10-01

    Full Text Available The present paper investigates the physical and mechanical modifications of both poplar (I-214 clone and ceiba veneers and plywood after heat treatments of different intensities (5 and 7 % of dry mass losses. Plywood panels were glued before and after heat treatment with urea-formaldehyde (UF and melamine-urea-formaldehyde (MUF resins. In order to assess the treatments’ effects on both the wood and the glues, the dry mass, the density, the bending strength, the Young’s modulus and the bonding quality were measured before and after heat treatment. The results of the different treatments were compared as well. Results showed that the loss in cell wall polymers due to the heat treatment caused a significant reduction of the equilibrium moisture content of the samples. From a mechanical point of view the treatment resulted in an important reduction of strength and in a small reduction of stiffness. Bonding quality as well as mechanical properties were widely affected by the heat treatment. The different intensities of the treatments (the treatment range was up to 5% and 7% of dry mass loss did not show significant differences for most of the features assessed. The mechanical performance as well as the bonding quality of treated samples suggested that veneers should be glued after heat treatment. Apparent cohesive wood failure showed that different degradations affect wood and glues with a prominent effect on the glues for UF resins and a prominent effect on the wood for MUF resins.

  8. Research of immobilized Candida rugosa lipase on ion exchange resin 214%214型离子交换树脂固定化假丝酵母脂肪酶的研究

    Institute of Scientific and Technical Information of China (English)

    钱俊青; 蒋盛蓝; 秦德怀; 郭辉; 凌春英

    2013-01-01

    以假丝酵母脂肪酶(Candida rugosa lipase)催化脂肪酸甲酯化是植物油精制副产物中提取天然维生素E的重要预处理反应.为提高预处理效果,通过固定化假丝酵母脂肪酶提高其酯化能力,降低水解反应的能力.笔者在不同性能树脂筛选的基础上,开展了离子交换树脂214型固定化假丝酵母脂肪酶的研究.以酶固定化率为指标,开展了酶质量浓度、树脂的量、缓冲液pH、固定化温度、时间和振荡速率等条件试验和响应面试验,得到的最适固定化条件为酶质量浓度6.09 mg/mL,5 mL酶液中加入0.75 g树脂,缓冲液pH 8.4,振荡转速134.26 r/min,固定化温度30℃,固定化时间4h.该条件下,假丝酵母脂肪酶与树脂的结合率最高,酶的水解作用基本消失,酯化能力得到保持,可以获得较好的维生素E提纯预处理效果.%Fatty acid catalytic esterification by Candida rugosa lipase is an important preconditioning response in the extraction of natural Vitamin E from by-products of vegetable oil refining.In order to improve the efficiency of pretreatment,immobilized lipase was applied to improve the esterification capacity and to reduce the hydrolysis reaction.214 ion exchange resin was selected to immobilize Candida rugosa lipase,the concentration of enzyme,the amount of the resin,buffer solution pH,temperature,time and oscillation rate condition tests and response surface test were made while the reaction rate of the immobilized enzymes was taken as an index.The optimum conditions were:30℃,0.75 g resin,6.09 mg/mL enzyme,pH 8.40,oscillation speed 134.26 r/min,4 h.Under this condition,the hydrolysis reaction was disappeared,immobilization rate reached a maximum.

  9. Novel ZEB2-BCL11B Fusion Gene Identified by RNA-Sequencing in Acute Myeloid Leukemia with t(2;14(q22;q32.

    Directory of Open Access Journals (Sweden)

    Synne Torkildsen

    Full Text Available RNA-sequencing of a case of acute myeloid leukemia with the bone marrow karyotype 46,XY,t(2;14(q22;q32[5]/47,XY,idem,+?4,del(6(q13q21[cp6]/46,XY[4] showed that the t(2;14 generated a ZEB2-BCL11B chimera in which exon 2 of ZEB2 (nucleotide 595 in the sequence with accession number NM_014795.3 was fused to exon 2 of BCL11B (nucleotide 554 in the sequence with accession number NM_022898.2. RT-PCR together with Sanger sequencing verified the presence of the above-mentioned fusion transcript. All functional domains of BCL11B are retained in the chimeric protein. Abnormal expression of BCL11B coding regions subjected to control by the ZEB2 promoter seems to be the leukemogenic mechanism behind the translocation.

  10. Wear behavior of 2-1/4 Cr-1 Mo tubing against alloy 718 tube-support material in sodium-cooled steam generators

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, W L

    1983-05-01

    A series of prototypic steam generator 2-{1/4} Cr-1 Mo tube/alloy 718 tube support plate wear tests were conducted in direct support of the Westinghouse Nuclear Components Division -- Breeder Reactor Components Project Large Scale steam Generator design. The initial objective was to verify the acceptable wear behavior of softer, over-aged'' alloy 718 support plate material. For all interfaces under all test conditions, resultant wear damage was adhesive in nature with varying amounts of 2-{1/4} Cr-1 Mo tube material being adhesively transferred to the alloy 718 tube supports. Maximum tube wear depths exceeded the initially established design allowable limit of 127 {mu}m (.005 in.) at 17 of the 18 interfaces tested. A decrease in contact stresses produced acceptable tube wear depths below a readjusted maximum design allowable value of 381 {mu}m (.015 in.). Additional conservatisms associated with the simulation of a 40-year lifetime of rubbing in a one-week laboratory test provided further confidence that the 381 {mu}m maximum tube wear allowance would not be exceeded in service. Softer, over-aged'' alloy 718 material was found to produce slightly less wear damage on 2-{1/4} Cr-1 Mo tubing than fully age hardened material. Also, air formed oxide films on the alloy 718 reduced initial tube wear and delayed the onset of adhesive surface damage. However, at high surface stress levels, these films were not sufficiently stable to provide adequate long term protection from adhesive wear. The results of the present work and those of previous test programs suggest that the successful in-sodium tribological performance of 2-{1/4} Cr-1 Mo/alloy 718 rubbing couples is dependent upon the presence of lubricative surface films, such as oxides and/or surface reaction or deposition products. 11 refs., 13 figs., 4 tabs.

  11. Study on Bone Mineral Density and its Influencing Factors among 1 214 Older Adults in Xuhui District, Shanghai

    Institute of Scientific and Technical Information of China (English)

    Jian-ping CHEN; Mao-hua MIAO; Li-feng ZHOU; Jie YANG; Guang-hua LU; Xiao-ping ZHOU; Hui-qin YU; Er-sheng GAO

    2006-01-01

    Objective To understand the status of the forearm bone mineral density (BMD) in older adults, and to identify the potentialfactors influencing BMD.Methods A total of 1 214 older adults were examined, in Tianlin, Fenglin and Xietu sub-districts, of Xuhui district, Shanghai. BMD was tested at the left forearm,including distal radius and ulna 1/3 total, distalradius where the distance of radius and ulna is 8 mm, ultra-distal radius, by single energy X-ray densitometer.Results The BMD at all of the four sites decreased with the age increased. The average BMD was higher in male than in female for the same age and measured site.Distal radius 1/3 total had the highest BMD among the four sites. The incidence of low BMD and osteoporosis in distal forearm 1/3 radius total ascended with the age increased.Multiplied logistic regression showed that males had a lower risk to be low BMD (OR=0.19) compared with female. Compared with the group aged from 50 to 54, the odds ratio in the group aged from 55 to 59, 60 to 64, and 65 to 70 were respectively 3.17,5.13, 15.03. Compared with those whose monthly salary was less than 1 000, the odds ratio was 0.70 in those whose salary was more than 1 000.Conclusion The incidence of low BMD was high in older adults, and it is related with sex, age, monthly salary.

  12. Twelve-year experience of radioembolization for colorectal hepatic metastases in 214 patients: survival by era and chemotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Lewandowski, Robert J.; Memon, Khairuddin; Hickey, Ryan; Gates, Vanessa L.; Atassi, Bassel; Vouche, Michael; Atassi, Rohi; Desai, Kush; Hohlastos, Elias; Sato, Kent; Habib, Ali [Robert H. Lurie Comprehensive Cancer Center, Department of Radiology, Section of Interventional Radiology, Northwestern Memorial Hospital, Chicago, IL (United States); Mulcahy, Mary F.; Kircher, Sheetal; Newman, Steven B.; Nimeiri, Halla; Benson, Al B. [Northwestern University, Department of Medicine, Division of Hematology and Oncology, Robert H. Lurie Comprehensive Cancer Center, Chicago, IL (United States); Marshall, Karen; Williams, Melissa; Salzig, Krystina; Salem, Riad [Robert H. Lurie Comprehensive Cancer Center, Department of Radiology, Section of Interventional Radiology, Northwestern Memorial Hospital, Chicago, IL (United States); Northwestern University, Department of Medicine, Division of Hematology and Oncology, Robert H. Lurie Comprehensive Cancer Center, Chicago, IL (United States)

    2014-10-15

    The aim of this study was to analyze the safety, treatment characteristics and survival outcomes of Yttrium-90 (Y90) radioembolization for unresectable colorectal carcinoma (CRC) liver metastases refractory to standard of care therapy. A total of 214 patients with CRC metastases were treated with Y90 radioembolization over 12 years. Toxicity was assessed using National Cancer Institute common terminology criteria. Overall survival was analyzed from date of diagnosis of primary cancer, hepatic metastases and from the first Y90. Uni/multivariate analyses were performed. Substratification by era of chemotherapeutics was performed. Most patients were male (60 %) and <65 years old (61 %). Of them, 98 % had been exposed to chemotherapy. Grade 3 lymphocyte, bilirubin, albumin, ALP and AST toxicities were observed in 39 %, 11 %, 10 %, 8 % and 4 % of patients, respectively. Grade 4 lymphocyte and ALP toxicities were observed in 5 % and 3 % of patients, respectively. Median overall survival was 43.0, 34.6, and 10.6 months from date of diagnosis of primary cancer, hepatic metastases and first Y90, respectively. Survival was significantly longer in patients: (1) who received ≤2 cytotoxic drugs (n = 104) than those who received 3 (n = 110) (15.2 vs. 7.5 months, p = 0.0001); and (2) who received no biologic agents (n = 52) compared with those that did (n = 162) (18.6 vs. 9.4 months, p = 0.0001). Multivariate analyses identified ≤2 cytotoxic agents, no exposure to biologics, ECOG 0, tumor burden <25 %, lack of extrahepatic disease and albumin >3 g/dL as independent predictors of survival. In this largest metastatic CRC series published to date, Y90 radioembolization was found to be safe; survival varied by prior therapy. Further studies are required to further refine the role of Y90 in metastatic CRC. (orig.)

  13. Comparative study of ion-exchange resin Indion 204 and Indion 214 for the taste masking of metoclopramide hydrochloride and formulation of rapid-disintegrating tablets

    Directory of Open Access Journals (Sweden)

    Dahima Rashmi

    2010-01-01

    Full Text Available The purpose of this research was to mask the intensely bitter taste of metoclopramide hydrochloride and to formulate a rapid-disintegrating tablet of the taste-masked drug. Taste masking was done by complexing the drug with ion exchange resin, Indion 204 and Indion 214, in different ratios. The complex loading process was optimized for the concentration of resin, swelling time, stirring time, pH, and temperature for maximum drug loading. Drug-resin complexes (DRC were tested for flow properties, drug content, in-vitro release in simulated salivary fluid, and in simulated gastric fluid (SGF, taste evaluation by the panel method. Taste evaluation of DRC revealed considerable taste masking with the degree of bitterness below threshold value (40 μg/ml in 0 to 5 min. Complex of both Indion 204 and Indion 214 masked the taste, but on the basis of the comparative study, resin 214 was selected for taste masking property. Disintegrant croscarmellose (5% wt/wt gave the minimum disintegration time in comparison to crosspovidone and sodium starch glycolate. The batch of tablet containing Pearlitol SD and Avicel (PH102 in the ratio 60:40 and 5% (wt/wt Croscarmellose showed faster disintegration i.e. 32 s, as compare to marketed tablet. It also revealed rapid drug release (t 80 , 6 min in SGF compared with marketed formulation (t 80 , 9 min.

  14. Production of actinium-225 for alpha particle mediated radioimmunotherapy.

    Science.gov (United States)

    Boll, Rose A; Malkemus, Dairin; Mirzadeh, Saed

    2005-05-01

    The initial clinical trials for treatment of acute myeloid leukemia have demonstrated the effectiveness of the alpha emitter (213)Bi in killing cancer cells. Bismuth-213 is obtained from a radionuclide generator system from decay of 10-days (225)Ac parent. Recent pre-clinical studies have also shown the potential application of both (213)Bi, and the (225)Ac parent radionuclide in a variety of cancer systems and targeted radiotherapy. This paper describes our five years of experience in production of (225)Ac in partial support of the on-going clinical trials. A four-step chemical process, consisting of both anion and cation exchange chromatography, is utilized for routine separation of carrier-free (225)Ac from a mixture of (228)Th, (229)Th and (232)Th. The separation of Ra and Ac from Th is achieved using the marcoporous anion exchange resin MP1 in 8M HNO(3) media. Two sequential MP1/NO(3) columns provide a separation factor of approximately 10(6) for Ra and Ac from Th. The separation of Ac from Ra is accomplished on a low cross-linking cation exchange resin AG50-X4 using 1.2M HNO(3) as eluant. Two sequential AG50/NO(3) columns provide a separation factor of approximately 10(2) for Ac from Ra. A 60-day processing schedule has been adopted in order to reduce the processing cost and to provide the highest levels of (225)Ac possible. Over an 8-week campaign, a total of approximately 100 mCi of (225)Ac (approximately 80% of the theoretical yield) is shipped in 5-6 batches, with the first batch typically consisting of approximately 50 mCi. After the initial separation and purification of Ac, the Ra pool is re-processed on a bi-weekly schedule or as needed to provide smaller batches of (225)Ac. The averaged radioisotopic purity of the (225)Ac was 99.6 +/- 0.7% with a (225)Ra content of < or =0.6%, and an average (229)Th content of (4(-4)(+5)) x 10(-5)%.

  15. Isolation of Actinium from Neutron-irradiated Thorium-I

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    Isolation of Actinium from Neutron-irradiated Thorium-I¥YangWeifan;YuanShuanggui;MuWantong;ZhangXueqian;LiZhongweiandZhaoLili...

  16. The underlying proantiarrhythmic mechanism of 5-HT_4 receptor agonist and 5-HT_3 receptor antagonist 2-[1-(4-piperonyl)piperazinyl]benzothiazole%5-HT_4受体激动剂兼5-HT_3 受体阻断剂2-[1-(4-piperonyl)piperazinyl]-benzothiazole致心律失常机制探析

    Institute of Scientific and Technical Information of China (English)

    刘清华; 张杨; 林媛媛; 吴博威

    2010-01-01

    目的 观察5-HT_4受体激动剂兼5-HT_3受体阻断剂2-[1-(4-piperonyl)piperazinyl]benzothiazole对大鼠离体心脏心律的影响,并探析其电生理学机制.方法 采用成年健康SD大鼠建立离体心脏Langendorff主动脉逆行灌流系统,观察0.1~10 μmol · L~(-1) 2-[1-(4-piperonyl)piperazinyl]benzothiazole对离体心脏节律的影响,全程记录心电图的变化.应用全细胞膜片钳技术观察2-[1-(4-piperonyl)piperazinyl]benzothiazole对胶原酶分解的大鼠心室肌细胞膜内向整流钾电流(I_(K1))、瞬时外向钾电流(I_(to))、静息膜电位(RMP)及动作电位(AP)的影响.结果 在大鼠离体心脏,0.1~10 μmol·L~(-1) 2-[1-(4-piperonyl)piperazinyl]benzothiazole可诱发明显的心律失常.给药15 min内,药物(10 μmol·L~(-1))诱发期前收缩(PVB)236±37个,室速(VT)和室颤(VF)发生率分别达到87.5%和62.5%(n=8,P<0.01).膜片钳记录结果显示,0.1~10 μmol·L~(-1) 2-[1-(4-piperonyl)piperazinyl]benzothiazole可浓度依赖性抑制大鼠心室肌IK1(EC50=0.74 μmol·L~(-1))和I_(to)(EC_(50)=2.16 μmol·L~(-1)),降低膜电位,并明显延长动作电位时程(n=6,P<0.01).结论 作为5-HT_4受体激动剂和5-HT_3受体阻断剂2-[1-(4-pipero-nyl)piperazinyl]benzothiazole致大鼠心律失常风险的电生理学机制为抑制I_(K1)和I_(to),降低膜电位,延长动作电位时程.

  17. A Functional Variant at the miR-214 Binding Site in the Methylenetetrahydrofolatereductase Gene Alters Susceptibility to Gastric Cancer in a Chinese Han Population

    Directory of Open Access Journals (Sweden)

    Qiaoyun Chen

    2015-05-01

    Full Text Available Background and Aims: Single nucleotide polymorphisms in miRNA binding sites, which are located in mRNA 3' untranslated regions (3'-UTRs, were recently found to influence microRNA-target interactions. Specifically, such polymorphisms can modulatebinding affinity or create or destroy miRNA-binding sites; such variants have also been found to be associated with cancer risk. In this study, we explored the effect of a functional variant at the miR-214 binding site in the methylenetetrahydrofolate reductase gene (rs114673809 on gastric cancer (GC risk in a hospital-based case-control study in a Chinese Han population. Methods and Results: We genotyped the rs114673809 polymorphism in 345 gastric cancer patients and 376 cancer-free controls using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP technique. The functions of rs114673809 were investigated using a luciferase activity assay and validated by immunoblotting. We found that participants carrying the rs114673809 AA genotype or A allele had a significantly increased risk of gastric cancer (OR = 1.667, 95% CI = 1.044-2.660, P = 0.034; OR = 1.261, 95% CI = 1.017-1.563, P = 0.037, respectively compared to those carrying the GG genotype and G allele. In addition, rs114673809 modified the binding of hsa-miR-214 to MTHFR as well as MTHFR protein levels in gastric cancer patients. Conclusion: Our data suggested that rs114673809, which is located at the miR-214 binding site in the 3'-UTR of MTHFR, may play an important role in the development of gastric cancer in a Chinese Han population.

  18. t(6;9)(p22;q34)/DEK-NUP214-rearranged pediatric myeloid leukemia: an international study of 62 patients.

    Science.gov (United States)

    Sandahl, Julie Damgaard; Coenen, Eva A; Forestier, Erik; Harbott, Jochen; Johansson, Bertil; Kerndrup, Gitte; Adachi, Souichi; Auvrignon, Anne; Beverloo, H Berna; Cayuela, Jean-Michel; Chilton, Lucy; Fornerod, Maarten; de Haas, Valérie; Harrison, Christine J; Inaba, Hiroto; Kaspers, Gertjan J L; Liang, Der-Cherng; Locatelli, Franco; Masetti, Riccardo; Perot, Christine; Raimondi, Susana C; Reinhardt, Katarina; Tomizawa, Daisuke; von Neuhoff, Nils; Zecca, Marco; Zwaan, C Michel; van den Heuvel-Eibrink, Marry M; Hasle, Henrik

    2014-05-01

    Acute myeloid leukemia with t(6;9)(p22;q34) is listed as a distinct entity in the 2008 World Health Organization classification, but little is known about the clinical implications of t(6;9)-positive myeloid leukemia in children. This international multicenter study presents the clinical and genetic characteristics of 62 pediatric patients with t(6;9)/DEK-NUP214-rearranged myeloid leukemia; 54 diagnosed as having acute myeloid leukemia, representing <1% of all childhood acute myeloid leukemia, and eight as having myelodysplastic syndrome. The t(6;9)/DEK-NUP214 was associated with relatively late onset (median age 10.4 years), male predominance (sex ratio 1.7), French-American-British M2 classification (54%), myelodysplasia (100%), and FLT3-ITD (42%). Outcome was substantially better than previously reported with a 5-year event-free survival of 32%, 5-year overall survival of 53%, and a 5-year cumulative incidence of relapse of 57%. Hematopoietic stem cell transplantation in first complete remission improved the 5-year event-free survival compared with chemotherapy alone (68% versus 18%; P<0.01) but not the overall survival (68% versus 54%; P=0.48). The presence of FLT3-ITD had a non-significant negative effect on 5-year overall survival compared with non-mutated cases (22% versus 62%; P=0.13). Gene expression profiling showed a unique signature characterized by significantly higher expression of EYA3, SESN1, PRDM2/RIZ, and HIST2H4 genes. In conclusion, t(6;9)/DEK-NUP214 represents a unique subtype of acute myeloid leukemia with a high risk of relapse, high frequency of FLT3-ITD, and a specific gene expression signature.

  19. A 4.1 keV spectral feature in a type 1 X-ray burst from EXO 1747-214

    Science.gov (United States)

    Magnier, Eugene; Lewin, Walter H. G.; Tan, Jianmin; Van Paradijs, Jan; Penninx, Wim

    1989-01-01

    A spectral analysis of two type 1 bursts from EXO 1747-214 detected by Exosat is presented. During the rise of the strongest burst, the spectrum display a spectral feature which is well represented by an absorption line at 4.05 + or - 0.12 keV with an equivalent width of 570 + or - 87 eV and an upper limit to the line width at 800 eV. At all other times, the spectrum is well represented by that of a single blackbody. The observed line energy is compared with those observed in other burst and the possible origins of these lines are discussed.

  20. Isotope Shifts of the $6d\\,^2$D$_{3/2}\\,$ - $7p\\,^2$P$_{1/2}\\,$ Transition in Trapped Short-Lived $^{209-214}$Ra$^+$

    CERN Document Server

    Giri, G S; Berg, J E van den; Böll, O; Dammalapati, U; van der Hoek, D J; Jungmann, K; Kruithof, W L; Müller, S; Portela, M Nuñez; Onderwater, C J G; Santra, B; Timmermans, R G E; Wansbeek, L W; Willmann, L; Wilschut, H W

    2011-01-01

    Laser spectroscopy of short-lived radium isotopes in a linear Paul trap has been performed. The isotope shifts of the $6d\\,^2$D$_{3/2}\\,$ - $7p\\,^2$P$_{1/2}\\,$ transition in $^{209-214}$Ra$^+$ were measured, which are sensitive to the short range part of the atomic wavefunctions. The results are essential experimental input for improving the precision of atomic structure calculation. This is indispensable for parity violation in Ra$^+$ aiming at the determination of the weak mixing angle.

  1. Multigenerational effects of whole body exposure to 2.14 GHz W-CDMA cellular phone signals on brain function in rats.

    Science.gov (United States)

    Shirai, Tomoyuki; Imai, Norio; Wang, Jianqing; Takahashi, Satoru; Kawabe, Mayumi; Wake, Kanako; Kawai, Hiroki; Watanabe, So-Ichi; Furukawa, Fumio; Fujiwara, Osamu

    2014-10-01

    The present experimental study was carried out with rats to evaluate the effects of whole body exposure to 2.14 GHz band code division multiple access (W-CDMA) signals for 20 h a day, over three generations. The average specific absorption rate (SAR, in unit of W/kg) for dams was designed at three levels: high (CDMA signals for 20 h/day did not cause any adverse effects on the F1 , F2 , and F3 offspring.

  2. Expression of Clu and Tgfb1 during murine tooth development: effects of in-vivo transfection with anti-miR-214.

    Science.gov (United States)

    Khan, Qalb-E-Saleem; Sehic, Amer; Khuu, Cuong; Risnes, Steinar; Osmundsen, Harald

    2013-08-01

    Expression of clusterin (Clu) in the murine first molar tooth germ was markedly increased at postnatal developmental stages. The time-course of expression of this gene paralleled those of other genes encoding proteins involved during the secretory phase of odontogenesis, as described previously. Immunohistochemical studies of clusterin in murine molar tooth germs suggested this protein to be located in outer enamel epithelium, regressing enamel organ, secretory ameloblasts, and the dental epithelium connecting the tooth to the oral epithelium at an early eruptive stage. Immunolabelling of transforming growth factor beta-1 (TGF-β1) revealed it to be located close to clusterin. The levels of expression of Clu and Tgfb1 were markedly decreased following in-vivo transfection with anti-miR-214. In contrast, the expression of several genes associated with regulation of growth and development were increased by this treatment. We suggest that clusterin has functions during secretory odontogenesis and the early eruptive phase. Bioinformatic analysis after treatment with anti-miR-214 suggested that, whilst cellular activities associated with tooth mineralization and eruption were inhibited, activities associated with an alternative developmental activity (i.e. biosynthesis of contractile proteins) appeared to be stimulated. These changes probably occur through regulation mediated by a common cluster of transcription factors and support suggestions that microRNAs (miRNAs) are highly significant as regulators of differentiation during odontogenesis. © 2013 Eur J Oral Sci.

  3. Micro RNA-214 contributes to proteasome independent downregulation of beta catenin in Huntington's disease knock-in striatal cell model STHdhQ111/Q111.

    Science.gov (United States)

    Ghatak, Supratim; Raha, Sanghamitra

    2015-04-10

    Role of beta catenin in Huntington's disease (HD) is not clear. Previous studies on HD reported varied levels of beta catenin. In the present study we showed that beta catenin is post transcriptionally down-regulated in mutant huntingtin knock-in cell model STHdhQ111/Q111. This in turn leads to decreased level of wnt/beta catenin responsive genes. We observed that Gsk3beta or Gsk3beta (phospho Ser 9) is unaltered in HD and this down-regulation of beta catenin is independent of proteasomal degradation. Finally, we showed that the overexpression of miR-214 leads to the down-regulation of beta catenin at protein level only and reduces its transcriptional activity. We concluded that, miR-214 contributes to the processes that result in proteasome independent post transcriptional down-regulation of beta catenin in STHdhQ111/Q111, probably through inhibition of protein synthesis from beta catenin mRNA. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. Results of a search for daily and annual variations of the Po-214 half-life at the two year observation period

    CERN Document Server

    Alexeyev, E N; Gangapshev, A M; Kazalov, V V; Kuzminov, V V; Panasenko, S I; Ratkevich, S S

    2015-01-01

    The brief description of installation TAU-2 intended for long-term monitoring of the half-life value $\\tau$ ($\\tau_{1/2}$) of the $^{214}$Po is presented. The methods of measurement and processing of collected data are reported. The results of analysis of time series values $\\tau$ with different time step are presented. Total of measurement time was equal to 590 days. Averaged value of the $^{214}$Po half-life was obtained $\\tau=163.46\\pm0.04$ $\\mu$s. The annual variation with an amplitude $A=(8.9\\pm2.3)\\cdot10^{-4}$, solar-daily variation with an amplitude $A_{So}=(7.5\\pm1.2)\\cdot10^{-4}$, lunar-daily variation with an amplitude $A_L=(6.9\\pm2.0)\\cdot10^{-4}$ and sidereal-daily variation with an amplitude $A_S=(7.2\\pm1.2)\\cdot10^{-4}$ were found in a series of $\\tau$ values. The maximal values of amplitude are observed at the moments when the projections of the installation Earth location velocity vectors toward the source of possible variation achieve its maximal magnitudes.

  5. Planck intermediate results. VI. The dynamical structure of PLCKG214.6+37.0, a Planck discovered triple system of galaxy clusters

    Science.gov (United States)

    Planck Collaboration; Ade, P. A. R.; Aghanim, N.; Arnaud, M.; Ashdown, M.; Atrio-Barandela, F.; Aumont, J.; Baccigalupi, C.; Balbi, A.; Banday, A. J.; Barreiro, R. B.; Bartlett, J. G.; Battaner, E.; Benabed, K.; Benoît, A.; Bernard, J.-P.; Bersanelli, M.; Bhatia, R.; Bikmaev, I.; Böhringer, H.; Bonaldi, A.; Bond, J. R.; Borrill, J.; Bouchet, F. R.; Bourdin, H.; Burenin, R.; Burigana, C.; Cabella, P.; Cardoso, J.-F.; Castex, G.; Catalano, A.; Cayón, L.; Chamballu, A.; Chiang, L.-Y.; Chon, G.; Christensen, P. R.; Clements, D. L.; Colafrancesco, S.; Colombi, S.; Colombo, L. P. L.; Comis, B.; Coulais, A.; Crill, B. P.; Cuttaia, F.; Da Silva, A.; Dahle, H.; Danese, L.; Davis, R. J.; de Bernardis, P.; de Gasperis, G.; de Zotti, G.; Delabrouille, J.; Démoclès, J.; Diego, J. M.; Dolag, K.; Dole, H.; Donzelli, S.; Doré, O.; Dörl, U.; Douspis, M.; Dupac, X.; Efstathiou, G.; Enßlin, T. A.; Eriksen, H. K.; Finelli, F.; Flores-Cacho, I.; Forni, O.; Frailis, M.; Franceschi, E.; Frommert, M.; Galeotta, S.; Ganga, K.; Génova-Santos, R. T.; Giard, M.; Gilfanov, M.; Giraud-Héraud, Y.; González-Nuevo, J.; Górski, K. M.; Gregorio, A.; Gruppuso, A.; Hansen, F. K.; Harrison, D.; Heinämäki, P.; Hempel, A.; Henrot-Versillé, S.; Hernández-Monteagudo, C.; Herranz, D.; Hildebrandt, S. R.; Hivon, E.; Hobson, M.; Holmes, W. A.; Hurier, G.; Jaffe, T. R.; Jaffe, A. H.; Jagemann, T.; Jones, W. C.; Juvela, M.; Keihänen, E.; Khamitov, I.; Kisner, T. S.; Kneissl, R.; Knoche, J.; Knox, L.; Kunz, M.; Kurki-Suonio, H.; Lagache, G.; Lähteenmäki, A.; Lamarre, J.-M.; Lasenby, A.; Lawrence, C. R.; Le Jeune, M.; Leonardi, R.; Lilje, P. B.; López-Caniego, M.; Luzzi, G.; Macías-Pérez, J. F.; Maino, D.; Mandolesi, N.; Maris, M.; Marleau, F.; Marshall, D. J.; Martínez-González, E.; Masi, S.; Massardi, M.; Matarrese, S.; Mazzotta, P.; Mei, S.; Melchiorri, A.; Melin, J.-B.; Mendes, L.; Mennella, A.; Mitra, S.; Miville-Deschênes, M.-A.; Moneti, A.; Montier, L.; Morgante, G.; Mortlock, D.; Munshi, D.; Murphy, J. A.; Naselsky, P.; Nati, F.; Natoli, P.; Nørgaard-Nielsen, H. U.; Noviello, F.; Novikov, D.; Novikov, I.; Osborne, S.; Pajot, F.; Paoletti, D.; Pasian, F.; Patanchon, G.; Perdereau, O.; Perotto, L.; Perrotta, F.; Piacentini, F.; Piat, M.; Pierpaoli, E.; Piffaretti, R.; Plaszczynski, S.; Pointecouteau, E.; Polenta, G.; Ponthieu, N.; Popa, L.; Poutanen, T.; Pratt, G. W.; Prunet, S.; Puget, J.-L.; Rachen, J. P.; Rebolo, R.; Reinecke, M.; Remazeilles, M.; Renault, C.; Ricciardi, S.; Riller, T.; Ristorcelli, I.; Rocha, G.; Roman, M.; Rosset, C.; Rossetti, M.; Rubiño-Martín, J. A.; Rusholme, B.; Sandri, M.; Savini, G.; Scott, D.; Smoot, G. F.; Starck, J.-L.; Sudiwala, R.; Sunyaev, R.; Sutton, D.; Suur-Uski, A.-S.; Sygnet, J.-F.; Tauber, J. A.; Terenzi, L.; Toffolatti, L.; Tomasi, M.; Tristram, M.; Tuovinen, J.; Valenziano, L.; Van Tent, B.; Vielva, P.; Villa, F.; Vittorio, N.; Wade, L. A.; Wandelt, B. D.; Welikala, N.; Yvon, D.; Zacchei, A.; Zaroubi, S.; Zonca, A.

    2013-02-01

    The survey of galaxy clusters performed by Planck through the Sunyaev-Zeldovich effect has already discovered many interesting objects, thanks to its full sky coverage. One of the SZ candidates detected inthe early months of the mission near to the signal-to-noise threshold, PLCKG214.6+37.0, was later revealed by XMM-Newton to be a triple system of galaxy clusters. We present the results from a deep XMM-Newton re-observation of PLCKG214.6+37.0, part of a multi-wavelength programme to investigate Planck discovered superclusters. The characterisation of the physical properties of the three components has allowed us to build a template model to extract the total SZ signal of this system with Planck data. We have partly reconciled the discrepancy between the expected SZ signal derived from X-rays and the observed one, which are now consistent within 1.2σ. We measured the redshift of the three components with the iron lines in the X-ray spectrum, and confirm that the three clumps are likely part of the same supercluster structure. The analysis of the dynamical state of the three components, as well as the absence of detectable excess X-ray emission, suggests that we are witnessing the formation of a massive cluster at an early phase of interaction.

  6. Saturation mutagenesis of Bradyrhizobium sp. BTAi1 toluene 4-monooxygenase at alpha-subunit residues proline 101, proline 103, and histidine 214 for regiospecific oxidation of aromatics.

    Science.gov (United States)

    Yanık-Yıldırım, K Cansu; Vardar-Schara, Gönül

    2014-11-01

    A novel toluene monooxygenase (TMO) six-gene cluster from Bradyrhizobium sp. BTAi1 having an overall 35, 36, and 38 % protein similarity with toluene o-xylene monooxygenase (ToMO) of Pseudomonas sp. OX1, toluene 4-monooxygenase (T4MO) of Pseudomonas mendocina KR1, and toluene-para-monooxygenase (TpMO) of Ralstonia pickettii PKO1, respectively, was cloned and expressed in Escherichia coli TG1, and its potential activity was investigated for aromatic hydroxylation and trichloroethylene (TCE) degradation. The natural substrate toluene was hydroxylated to p-cresol, indicating that the new toluene monooxygenase (T4MO·BTAi1) acts as a para hydroxylating enzyme, similar to T4MO and TpMO. Some shifts in regiospecific hydroxylations were observed compared to the other wild-type TMOs. For example, wild-type T4MO·BTAi1 formed catechol (88 %) and hydroquinone (12 %) from phenol, whereas all the other wild-type TMOs were reported to form only catechol. Furthermore, it was discovered that TG1 cells expressing wild-type T4MO·BTAi1 mineralized TCE at a rate of 0.67 ± 0.10 nmol Cl(-)/h/mg protein. Saturation and site directed mutagenesis were used to generate eight variants of T4MO·BTAi1 at alpha-subunit positions P101, P103, and H214: P101T/P103A, P101S, P101N/P103T, P101V, P103T, P101V/P103T, H214G, and H214G/D278N; by testing the substrates phenol, nitrobenzene, and naphthalene, positions P101 and P103 were found to influence the regiospecific oxidation of aromatics. For example, compared to wild type, variant P103T produced four fold more m-nitrophenol from nitrobenzene as well as produced mainly resorcinol (60 %) from phenol whereas wild-type T4MO·BTAi1 did not. Similarly, variants P101T/P103A and P101S synthesized more 2-naphthol and 2.3-fold and 1.6-fold less 1-naphthol from naphthalene, respectively.

  7. The rate commitment to ISO 214 standard among the persian abstracts of approved research projects at school of health management and medical informatics, Isfahan University of Medical Sciences, Isfahan, Iran.

    Science.gov (United States)

    Papi, Ahmad; Khalaji, Davoud; Rizi, Hasan Ashrafi; Shabani, Ahmad; Hassanzadeh, Akbar

    2014-01-01

    Commitment to abstracting standards has a very significant role in information retrieval. The present research aimed to evaluate the rate of Commitment to ISO 214 Standard among the Persian abstracts of approved research projects at School of Health Management and Medical Informatics, Isfahan University of Medical Sciences, Isfahan, Iran. This descriptive study used a researcher-made checklist to collect data, which was then analyzed through content analysis. The studied population consisted of 227 approved research projects in the School of Health Management and Medical Informatics, Isfahan University of Medical Sciences during 2001-2010. The validity of the checklist was measured by face and content validity. Data was collected through direct observations. Statistical analyzes including descriptive (frequency distribution and percent) and inferential statistics (Chi-square test) were performed in SPSS-16. The highest and lowest commitment rates to ISO 214 standard were in using third person pronouns (100%) and using active verbs (34/4%), respectively. In addition, the highest commitment rates to ISO 214 standard (100%) related to mentioning third person pronouns, starting the abstract with a sentence to explain the subject of the research, abstract placement, and including keyword in 2009. On the other hand, during 2001-2003, the lowest commitment rate was observed in reporting research findings (16/7%). Moreover, various educational groups differed significantly only in commitment to study goals, providing research findings, and abstaining from using abbreviations, signs, and acronyms. Furthermore, educational level of the corresponding author was significantly related with extracting the keywords from the text. Other factors of ISO 214 standard did not have significant relations with the educational level of the corresponding author. In general, a desirable rate of commitment to ISO 214 standard was observed among the Persian abstracts of approved research

  8. A novel familial 11p15.4 microduplication associated with intellectual disability, dysmorphic features, and obesity with involvement of the ZNF214 gene.

    Science.gov (United States)

    Sofos, Elvera; Pescosolido, Matthew F; Quintos, Jose B; Abuelo, Dianne; Gunn, Shelly; Hovanes, Karine; Morrow, Eric M; Shur, Natasha

    2012-01-01

    We evaluated a patient with mild intellectual disability, obesity, overgrowth, and dysmorphic features. Array comparative genomic hybridization (aCGH) analysis showed a single copy number increase of a BAC clone in the 11p15.4 region. Oligonucleotide aCGH refined the duplication to approximately 2.29  megabases (Mb) in size. Testing the parents revealed that the father, who had learning disabilities and overgrowth, also had the 11p15.4 duplication, and the mother had a normal microarray. In addition, the patient's brother and grandmother all share clinical features with the proband and tested positive for the duplication. The duplicated region (Chr11:6,934,067-9,220,605) encompasses 29 genes, including the ZNF214 gene, which has been postulated to play a role in Beckwith-Wiedemann syndrome [Alders et al., 2000]. This three-generation pedigree outlines features of a novel microduplication syndrome.

  9. Functional and radiographic evaluation of 214 aggressive benign bone lesions treated with curettage, cauterization, and cementation: 24 years of follow-up

    Directory of Open Access Journals (Sweden)

    Camargo Olavo Pires de

    2005-01-01

    Full Text Available PURPOSE: Treatment with curettage, cauterization, and methylmethacrylate of aggressive benign bone lesions is a method now widely accepted in most orthopedic oncology centers. However, one of the controversies regarding this technique is the possible complications that may arise from the use of methylmethacrylate, which has caused some authors to remove it 2 years after the surgery and replace it with bone graft. The objective of this paper is to present a functional and radiographic evaluation of 214 patients presenting with aggressive benign bone lesions treated with curettage, cauterization, and methylmethacrylate from 1974 to 1998, with some of them having 24 years of follow-up. These patients were clinically and radiographically evaluated for the incidence of late osteoarthrosis, range of motion, and pain in the involved joint. METHOD: This study comprised 214 patients, with an average follow-up duration of 10.6 years (range: 2 to 24 years. All cases involved aggressive benign lesions. The patients were evaluated according to the Musculoskeletal Tumor Society Score (MSTS functional evaluation system, and the complications are described. RESULTS: The MSTS functional evaluation was excellent in 166 cases (78%, good in 26 (12%, fair in 11 (5%, and poor in 11 (5%. The following complications were observed: late osteoarthrosis, 25 cases (12%; infection, 12 (6%; pathologic fracture, 11 (5%; and local recurrence, 19 (9%. CONCLUSION: Based on clinical assessment, no significant deleterious effects directly related to the use of methylmethacrylate were observed. The functional evaluation performed in 1998 (up to 24 years of follow-up did not show significant change when compared to the evaluation performed in 1985.

  10. Prediction of Molar Extinction Coefficients of Proteins and Peptides Using UV Absorption of the Constituent Amino Acids at 214 nm To Enable Quantitative Reverse Phase High-Performance Liquid Chromatography-Mass Spectrometry Analysis

    NARCIS (Netherlands)

    Kuipers, B.J.H.; Gruppen, H.

    2007-01-01

    The molar extinction coefficients of 20 amino acids and the peptide bond were measured at 214 nm in the presence of acetonitrile and formic acid to enable quantitative comparison of peptides eluting from reversed-phase high-performance liquid chromatography, once identified with mass spectrometry (R

  11. Prediction of Molar Extinction Coefficients of Proteins and Peptides Using UV Absorption of the Constituent Amino Acids at 214 nm To Enable Quantitative Reverse Phase High-Performance Liquid Chromatography-Mass Spectrometry Analysis

    NARCIS (Netherlands)

    Kuipers, B.J.H.; Gruppen, H.

    2007-01-01

    The molar extinction coefficients of 20 amino acids and the peptide bond were measured at 214 nm in the presence of acetonitrile and formic acid to enable quantitative comparison of peptides eluting from reversed-phase high-performance liquid chromatography, once identified with mass spectrometry

  12. Sulforaphane inhibits cancer stem-like cell properties and cisplatin resistance through miR-214-mediated downregulation of c-MYC in non-small cell lung cancer

    Science.gov (United States)

    Wu, Yue; Li, Lin-Lin; Liu, Ying; Miao, Xiao-Bo; Liu, Qiu-Zhen; Yao, Kai-Tai; Xiao, Guang-Hui

    2017-01-01

    We herein report that sulforaphane (SFN), a potent anti-cancer and well-tolerated dietary compound, inhibits cancer stem-like cell (CSC) properties and enhances therapeutic efficacy of cisplatin in human non-small cell lung cancer (NSCLC). SFN exerted these functions through upregulation of miR-214, which in turn targets the coding region of c-MYC. This finding was further corroborated by our observations that plasmid or lentiviral vector-mediated expression of 3'UTR-less c-MYC cDNA and cisplatin- or doxorubicin-induced endogenous c-MYC accumulation was similarly suppressed by either SFN or miR-214. Further, we showed that the reported inhibitory effects of SFN on β-catenin are also mediated by miR-214. SFN/miR-214 signaling inhibited CSC properties and enhanced the cytotoxicity of chemotherapeutic drugs in vitro. Experiments with nude mice carrying xenograft tumors showed that SFN sensitized NSCLC cells to cisplatin's efficacy, which is accompanied by inhibition of cisplatin-induced c-MYC accumulation in tumor tissues. Our results provided strong evidence and mechanisms to support consideration of SFN or synthetic derivatives as a therapeutic agent in combination with cisplatin for the treatment of patients with NSCLC and, potentially, other types of c-MYC-addicted tumors. PMID:28076844

  13. Prediction of Molar Extinction Coefficients of Proteins and Peptides Using UV Absorption of the Constituent Amino Acids at 214 nm To Enable Quantitative Reverse Phase High-Performance Liquid Chromatography-Mass Spectrometry Analysis

    NARCIS (Netherlands)

    Kuipers, B.J.H.; Gruppen, H.

    2007-01-01

    The molar extinction coefficients of 20 amino acids and the peptide bond were measured at 214 nm in the presence of acetonitrile and formic acid to enable quantitative comparison of peptides eluting from reversed-phase high-performance liquid chromatography, once identified with mass spectrometry (R

  14. Catalysis of atomic hydrogen to novel hydrogen species H{sup -}(1/4) and H{sub 2}(1/4) as a new power source

    Energy Technology Data Exchange (ETDEWEB)

    Mills, R.; He, J.; Chang, Z.; Good, W.; Lu, Y.; Dhandapani, B. [BlackLight Power, Inc., 493 Old Trenton Road, Cranbury, NJ 08512 (United States)

    2007-09-15

    The data from a broad spectrum of investigational techniques strongly and consistently indicate that hydrogen can exist in lower-energy states than previously thought possible. The predicted reaction involves a resonant, nonradiative energy transfer from otherwise stable atomic hydrogen to a catalyst capable of accepting the energy. The product is H(1/p), fractional Rydberg states of atomic hydrogen wherein n={l_brace}1/2{r_brace},{l_brace}1/3{r_brace},{l_brace}1/4{r_brace},..,1/p (p{<=}137 is an integer) replaces the well-known parameter n=integer in the Rydberg equation for hydrogen excited states. He{sup +}, Ar{sup +}, and K are predicted to serve as catalysts since they meet the catalyst criterion - a chemical or physical process with an enthalpy change equal to an integer multiple of the potential energy of atomic hydrogen, 27.2 eV. Specific predictions based on closed-form equations for energy levels were tested. For example, two H(1/p) may react to form H{sub 2}(1/p) that have vibrational and rotational energies that are p{sup 2} times those of H{sub 2} comprising uncatalyzed atomic hydrogen. Rotational lines were observed in the 145-300 nm region from atmospheric pressure electron-beam-excited argon-hydrogen plasmas. The unprecedented energy spacing of 4{sup 2} times that of hydrogen established the internuclear distance as 1/4 that of H{sub 2} and identified H{sub 2}(1/4). The predicted products of alkali catalyst K are H{sup -}(1/4) which form KH{sup *}X, a novel alkali halido (X) hydride compound, and H{sub 2}(1/4) which may be trapped in the crystal. The {sup 1}H MAS NMR spectrum of novel compound KH{sup *}Cl relative to external tetramethylsilane (TMS) showed a large distinct upfield resonance at -4.4ppm corresponding to an absolute resonance shift of -35.9ppm that matched the theoretical prediction of H(1/4) with p=4. The predicted frequencies of ortho- and para-H{sub 2}(1/4) were observed at 1943 and 2012cm{sup -1} in the high-resolution FTIR

  15. Electro-acupuncture-modulated miR-214 prevents neuronal apoptosis by targeting Bax and inhibits sodium channel Nav1.3 expression in rats after spinal cord injury.

    Science.gov (United States)

    Liu, Jing; Wu, Yaochi

    2017-03-11

    Electro-acupuncture (EA) has been proven to contribute towards neurologic and functional recoveries in spinal cord injury (SCI), but the underlying mechanism remains largely unknown especially regarding the effects of preventing neuronal apoptosis and alleviating neuropathic pain involved in the development of EA. In this study, we evaluated the effect of EA treatment in an animal model of SCI using the Basso, Beattie, and Bresnahan (BBB) score method, lesion volume by cresyl violet staining and neuronal apoptosis by TUNEL staining. Our results showed that EA therapy improved functional recovery, and reduced tissue loss and neuronal apoptosis after SCI. Meanwhile, we found that proapoptotic proteins (cleaved-caspase-3, 9 and cleaved-PARP) were downregulated and antiapoptotic protein Bcl-2 was upregulated following EA. To further explore the antiapoptotic effect of EA treatment, we verified that a large set of microRNAs (miRNAs) expression were altered following EA treatment and the miR-214 was one of the miRNAs being most significantly upregulated. Importantly, we validated both apoptosis related protein Bax and pain related protein Nav1.3 as two functional targets of miR-214 in vitro and vivo. Furthermore, our data showed that EA attenuates SCI-induced Nav1.3 and Bax upregulation in injured spinal cord via upregulating miR-214. These results suggest that miR-214 played an important role after SCI in the process of EA therapy, and the miR-214 could become an attractive novel therapeutic target for the treatment of SCI.

  16. MicroRNA-98 and microRNA-214 post-transcriptionally regulate enhancer of zeste homolog 2 and inhibit migration and invasion in human esophageal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Huang Sheng-Dong

    2012-08-01

    Full Text Available Abstract Background The enhancer of zeste homolog 2 (EZH2 was found to be overexpressed and associated with tumor metastasis in esophageal squamous cell carcinoma (ESCC. On the other hand, it was reported that miR-26a, miR-98, miR-101, miR-124, miR-138 and miR-214 could inhibit the expression of EZH2 in some tumors. However, the role of miRNAs in the regulation of EZH2 expression in human ESCC has not been documented. The aim of this study was to determine the role of these miRNAs in the regulation of tumor metastasis via EZH2 overexpression in human ESCC. Methods and results The expression of these miRNAs and EZH2 mRNA were examined by qPCR and the expression of EZH2 protein was detected by western blot. The role of these miRNAs in migration and invasion was studied in ESCC cell line (Eca109 transfected with miRNA mimics or cotransfected with miRNA mimics and pcDNA-EZH2 plasmid (without the 3’-UTR of EZH2. Through clinical investigation, we found that miR-98 and miR-214 expression was significantly lower in ESCC tissues than in matched normal tissues, and the expression level of miR-98 and miR-214 was inversely correlated to EZH2 protein expression and the clinical features such as pathological grade, tumor stage and lymph node metastasis in ESCC. In Eca109 cells, overexpression of miR-98 and miR-214 significantly inhibited the migration and invasion of ESCC cells, which was reversed by transfection of EZH2. Conclusions These findings suggest that decreased expression of miR-98 and miR-214 might promote metastasis of human ESCC by inducing accumulation of EZH2 protein.

  17. A Strategy for Interpretation of Microearthquake Tomography Results in the Salton Sea Geothermal Field Based upon Rock Physics Interpretations of State 2-14 Borehole Logs

    Energy Technology Data Exchange (ETDEWEB)

    Bonner, B; Hutchings, L; Kasameyer, P

    2006-06-14

    We devise a strategy for analysis of Vp and Vs microearthquake tomography results in the Salton Sea geothermal field to identify important features of the geothermal reservoir. We first interpret rock properties in State 2-14 borehole based upon logged core through the reservoir. Then, we interpret seismic recordings in the well (Daley et al., 1988) to develop the strategy. We hypothesize that mapping Poisson's ratio has two applications for the Salton Sea geothermal reservoir: (1) to map the top of the reservoir, and (2) as a diagnostic for permeable zones. Poisson's ratio can be obtained from Vp and Vs. In the State 2-14 borehole, Poisson's ratio calculated from large scale averages ({approx} 150 m) shows a monotonic decrease with depth to about 1300 m, at which point it increases with depth. Our model is that the monotonic decrease is due to compaction, and the increase below 1300 m is due to the rocks being hydrothermally altered. We hypothesize we can map the depth to alteration by identifying the transition from decreasing to increasing values; and thus, map the top of the reservoir, which is associated with a known increase in sulfite, chlorite, and epidote alteration that may be indicative of hydrothermal activity. We also observe (from Daley et. al. plots) an anomalous drop in Poisson's ratio at a depth of about 900 m, within a sandstone formation. The sandstone has a P-wave velocity significantly higher than the siltstone above it but a lower velocity in the lower half of the formation relative to the upper half. We interpret the relative decrease in velocity to be due to fracturing and chemical alteration caused by permeability. We conclude that using Vp and Vs tomography results to obtain images of Poisson's ratio has the potential to identify significant features in the geothermal reservoir in this geologic setting. Seismic attenuation tomography results (mapped as Qp and Qs) should also be useful for evaluating geothermal

  18. Study of the background of the neutrinoless double {beta} decay with the detector NEMO 2: contribution arising from the radon diffusion and internal pollution of the source {sup 214}Bi have been estimated; Etude du bruit de fond de la double-desintegration {beta} sans emission de neutrino dans le detecteur NEMO 2: contribution du radon ambiant et mesure de la pollution interne de la source en {sup 214}Bi

    Energy Technology Data Exchange (ETDEWEB)

    Mauger, F.

    1995-02-01

    The NEMO experiment is designed to understand the nature of the neutrino by studying the double beta decay of Mo-100 which is related to the Majorana neutrino effective mass. In this kind of experiment a good understanding of the different sources of background is crucial as only few events are expected per year at the required level of sensitivity. In this thesis we present the main theoretical and experimental aspects of the measurement of the neutrinoless double beta decay of Mo-100 with the prototype detector NEMO2. The goal of this study is to obtain a realistic interpretation of the few events detected at high energy in the two-electron channel as a background to neutrinoless double beta decay. In particular, the contribution arising from Bi-214 has been investigated. These events have been selected and analysed by means of the beta-alpha decays of Bi-214 into Pb-210. The events are characterized by a delayed track in the wire chamber and the corresponding signal is rather clean. The study has demonstrated the diffusion of Rn-222 into the detector and its contribution to Bi-214 pollution has been estimated. A measurement of the Bi-214 internal contamination of the source has been made as well as an estimation of the Bi-214 deposit due to Rn-222. As a result of this study it appears that, under the conditions of the NEMO2 experiment, the Bi and Rn contributions are of the same order of magnitude as the background induced at high energy by two-neutrino double beta decay. In conclusion, the backgrounds of the neutrinoless double beta decay of Mo-100 are well understood in the NEMO2 experiment leading to an extrapolation for the NEMO3 experiment. (authors).

  19. Novel PSEN1 mutations (H214N and R220P) associated with familial Alzheimer's disease identified by targeted exome sequencing.

    Science.gov (United States)

    Piccoli, Elena; Rossi, Giacomina; Rossi, Tommaso; Pelliccioni, Giuseppe; D'Amato, Ilaria; Tagliavini, Fabrizio; Di Fede, Giuseppe

    2016-04-01

    Autosomal dominant Alzheimer's disease (AD) is caused by mutations in amyloid precursor protein, presenilin 1 (PSEN1), and presenilin 2 genes and is mostly associated with early-onset form of AD (EOAD), whereas very few mutations were also found in late-onset AD (LOAD) cases. Because of the clinical overlapping between AD and other degenerative dementias such as frontotemporal dementias, a wide-spectrum genetic analysis should be envisaged in the differential diagnosis of this group of disorders. We used next-generation sequencing techniques to analyze 10 genes involved in dementia on a cohort of 20 EOAD and 20 LOAD cases. We found 5 rare coding variants (frequency <1%). PSEN1 H214N mutation, identified in a case of familial EOAD and PSEN1 R220P, found in a case of familial LOAD, are predicted to be pathogenic. These findings confirm the contribution of PSEN1 genetic variants also to LOAD, underlining the need of extending the genetic screening of presenilin mutations to LOAD patients. Two variants in microtubule-associated protein tau and 1 in progranulin appeared to be benign polymorphisms, showing no major contribution of these genes to AD.

  20. Use of a dynamic self-regulating prosthesis (P.A.D.) in inguinal hernia repair: our first experience in 214 patients.

    Science.gov (United States)

    Ferranti, Fabrizio; Marzano, Marco; Quintiliani, Alberto

    2009-01-01

    Numerous techniques exist for inguinal hernia treatment. Currently, open mesh tension-free repair is regarded as the repair method of choice. In particular Lichtenstein repair is the most common procedure performed, although several articles have reported long-lasting postoperative pain and a higher recurrence rate than originally reported. This study describes the P.A.D. (Protesi Autoregolantesi Dinamica) prosthesis implantation technique and reports postoperative complications and long-term results. From June 2002 to May 2005 a total of 214 patients underwent P.A.D. prosthesis inguinal repair. All patients were male, with a mean age of 51 years. All hernias were treated via an open inguinal approach using the original technique described by Valenti, with slight modifications. A total of 171'patients (80%) were available to follow-up 3 years after surgery. Early postoperative complications occurred in 14 patients (8.4%). Four patients (12.1%), who had undergone regional anaesthesia, developed urinary retention. Wound infection occurred in 3 patients (1.4%). There were two direct recurrences (0.93%) whereas chronic postoperative inguinal pain was reported in 4.2% of patients. Within the limitations of a short follow-up, our results show that the P.A.D. prosthesis procedure is a reliable technique with a low recurrence rate and low postoperative morbidity.

  1. Size dependent structural, electronic, and magnetic properties of Sc(N) (N=2-14) clusters investigated by density functional theory.

    Science.gov (United States)

    Bhunia, Snehasis; Vyas, Nidhi; Sahu, Chandan; Ojha, Animesh K

    2014-11-01

    Structural, electronic, and magnetic properties of ScN (N=2-14) clusters have been investigated using density functional theory (DFT) calculations. Different spin states isomer for each cluster size has been optimized with symmetry relaxation. The structural stability, dissociation energy, binding energy, spin stability, vertical ionization energy, electron affinity, chemical hardness, and size dependent magnetic moment per atom are calculated for the energetically most stable spin isomer for each size. The structural stability for a specific size cluster has been explained in terms of atomic shell closing effect, close packed symmetric structure, and chemical bonding. Spin stability of each cluster size is determined by calculating the value of spin gaps. The maximum value for second-order energy difference is observed for the clusters of size N = 2, 6, 11, and 13, which implies that these clusters are relatively more stable. The magnetic moment per atom corresponding to lowest energy structure has also been calculated. The magnetic moment per atom corresponding to lowest energy structures has been calculated. The calculated values of magnetic moment per atom vary in an oscillatory fashion with cluster size. The calculated results are compared with the available experimental data.

  2. Nature and Significance of Igneous Rocks Cored in the State 2-14 Research Borehole: Salton Sea Scientific Drilling Project, California

    Science.gov (United States)

    Herzig, Charles T.; Elders, Wilfred A.

    1988-11-01

    The State 2-14 research borehole of the Salton Sea Scientific Drilling Project penetrated 3.22 km of Pleistocene to Recent sedimentary rocks in the Salton Sea geothermal system, located in the Salton Trough of southern California and northern Baja California, Mexico. In addition, three intervals of igneous rocks were recovered; a silicic tuff and two sills of altered diabase. The chemical composition of the silicic tuff at 1704 m depth suggests that it is correlative with the Durmid Hill tuff, cropping out 25 km NW of the geothermal system. In turn, both of these tuffs may be deposits of the Bishop Tuff, erupted from the Long Valley caldera of central California at 0.7 Ma. The diabases are similar to basaltic xenoliths found in the nearby Salton Buttes rhyolite domes. These diabase are interpreted as hypabyssal intrusions resulting from magmatism due to rifting of the Salton Trough as part of the East Pacific Rise/Gulf of California transtensional system. The sills apparently intruded an already developed geo-thermal system and were in turn altered by it.

  3. Planck intermediate results. VI: The dynamical structure of PLCKG214.6+37.0, a Planck discovered triple system of galaxy clusters

    CERN Document Server

    Ade, P A R; Arnaud, M; Ashdown, M; Atrio-Barandela, F; Aumont, J; Baccigalupi, C; Balbi, A; Banday, A J; Barreiro, R B; Bartlett, J G; Battaner, E; Benabed, K; Benoît, A; Bernard, J -P; Bersanelli, M; Bhatia, R; Böhringer, H; Bonaldi, A; Bond, J R; Borrill, J; Bouchet, F R; Bourdin, H; Burigana, C; Cabella, P; Cardoso, J -F; Castex, G; Catalano, A; Cayón, L; Chamballu, A; Chiang, L -Y; Chon, G; Christensen, P R; Clements, D L; Colafrancesco, S; Colombi, S; Colombo, L P L; Comis, B; Coulais, A; Crill, B P; Cuttaia, F; Da Silva, A; Dahle, H; Danese, L; Davis, R J; de Bernardis, P; de Gasperis, G; de Zotti, G; Delabrouille, J; Diego, J M; Dolag, K; Dole, H; Donzelli, S; Doré, O; Dörl, U; Douspis, M; Dupac, X; Efstathiou, G; Enßlin, T A; Eriksen, H K; Finelli, F; Flores-Cacho, I; Forni, O; Frailis, M; Franceschi, E; Frommert, M; Galeotta, S; Ganga, K; Génova-Santos, R T; Giard, M; Gilfanov, M; Giraud-Héraud, Y; González-Nuevo, J; Górski, K M; Gregorio, A; Gruppuso, A; Hansen, F K; Harrison, D; Heinämäki, P; Hempel, A; Henrot-Versillé, S; Hernández-Monteagudo, C; Herranz, D; Hildebrandt, S R; Hivon, E; Hobson, M; Holmes, W A; Hurier, G; Jaffe, T R; Jaffe, A H; Jagemann, T; Jones, W C; Juvela, M; Keihänen, E; Kisner, T S; Kneissl, R; Knoche, J; Knox, L; Kunz, M; Kurki-Suonio, H; Lagache, G; Lähteenmäki, A; Lamarre, J -M; Lasenby, A; Lawrence, C R; Jeune, M Le; Leonardi, R; Lilje, P B; López-Caniego, M; Luzzi, G; Macías-Pérez, J F; Maino, D; Mandolesi, N; Maris, M; Marleau, F; Marshall, D J; Martínez-González, E; Masi, S; Massardi, M; Matarrese, S; Mazzotta, P; Mei, S; Melchiorri, A; Melin, J -B; Mendes, L; Mennella, A; Mitra, S; Miville-Deschênes, M -A; Moneti, A; Montier, L; Morgante, G; Mortlock, D; Munshi, D; Murphy, J A; Naselsky, P; Nati, F; Natoli, P; Nørgaard-Nielsen, H U; Noviello, F; Osborne, S; Pajot, F; Paoletti, D; Pasian, F; Patanchon, G; Perdereau, O; Perotto, L; Perrotta, F; Piacentini, F; Piat, M; Pierpaoli, E; Piffaretti, R; Plaszczynski, S; Pointecouteau, E; Polenta, G; Ponthieu, N; Popa, L; Poutanen, T; Pratt, G W; Prunet, S; Puget, J -L; Rachen, J P; Rebolo, R; Reinecke, M; Remazeilles, M; Renault, C; Ricciardi, S; Riller, T; Ristorcelli, I; Rocha, G; Roman, M; Rosset, C; Rossetti, M; Rubiño-Martín, J A; Rusholme, B; Sandri, M; Savini, G; Scott, D; Smoot, G F; Starck, J -L; Sudiwala, R; Sunyaev, R; Sutton, D; Suur-Uski, A -S; Sygnet, J -F; Tauber, J A; Terenzi, L; Toffolatti, L; Tomasi, M; Tristram, M; Tuovinen, J; Valenziano, L; Van Tent, B; Vielva, P; Villa, F; Vittorio, N; Wade, L A; Wandelt, B D; Welikala, N; Yvon, D; Zacchei, A; Zaroubi, S; Zonca, A

    2012-01-01

    The survey of galaxy clusters performed by Planck through the Sunyaev-Zeldovich effect has already discovered many interesting objects, thanks to the whole coverage of the sky. One of the SZ candidates detected in the early months of the mission near to the signal to noise threshold, PLCKG214.6+37.0, was later revealed by XMM-Newton to be a triple system of galaxy clusters. We have further investigated this puzzling system with a multi-wavelength approach and we present here the results from a deep XMM-Newton re-observation. The characterisation of the physical properties of the three components has allowed us to build a template model to extract the total SZ signal of this system with Planck data. We partly reconciled the discrepancy between the expected SZ signal from X-rays and the observed one, which are now consistent at less than 1.2 sigma. We measured the redshift of the three components with the iron lines in the X-ray spectrum, and confirmed that the three clumps are likely part of the same superclus...

  4. Molecular characterization of a 21.4 kilobase antibiotic resistance plasmid from an α-hemolytic Escherichia coli O108:H- human clinical isolate.

    Directory of Open Access Journals (Sweden)

    Fay E Dawes

    Full Text Available This study characterizes the 21.4 kilobase plasmid pECTm80 isolated from Escherichia coli strain 80, an α hemolytic human clinical diarrhoeal isolate (serotype O108:H-. DNA sequence analysis of pECTm80 revealed it belonged to incompatibility group X1, and contained plasmid partition and toxin-antitoxin systems, an R6K-like triple origin (ori replication system, genes required for replication regulation, insertion sequences IS1R, ISEc37 and a truncated transposase gene (Tn3-like ΔtnpA of the Tn3 family, and carried a class 2 integron. The class 2 integron of pECTm80 contains an intact cassette array dfrA1-sat2, encoding resistance to trimethoprim and streptothricin, and an aadA1 gene cassette truncated by the insertion of IS1R. The complex plasmid replication system includes α, β and γ origins of replication. Pairwise BLASTn comparison of pECTm80 with plasmid pE001 reveals a conserved plasmid backbone suggestive of a common ancestral lineage. Plasmid pECTm80 is of potential clinical importance, as it carries multiple genes to ensure its stable maintenance through successive bacterial cell divisions and multiple antibiotic resistance genes.

  5. Composite Cathode Bi1.14Sr0.43O2.14-Ag for Intermediate-temperature Solid Oxide Fuel Cells

    Institute of Scientific and Technical Information of China (English)

    GAO Zhan; ZHANG Ping; GAO Ruifeng; HUANG Jianbing; MAO Zongqiang

    2008-01-01

    Composites consisting of strontium stabilized bismuth oxide (Bi1.14Sr0.43O2.14 SSB) and silver were investigated as cathodes for intermediate-temperature solid oxide fuel cells with doped ceria electrolyte. There were no chemical reactions between the two components. The microstructure of the interfaces between composite cathodes and Ce0.8Sm0.2O1.9 (SDC) electrolytes was examined by scanning electron microscopy (SEM). Impedance spectroscopy measurements show that the performance of cathode fired at 700℃ is the best. When the content of Ag2O is 70wt%, polarization resistance values for the SSB-Ag cathodes are as low as 0.2Ωcm2 at 700℃ and 0.29Ωcm2 at 650℃. These results are much smaller than some of other reported composite cathodes on doped ceria electrolyte and indicate that SSB-Ag composite is a potential cathode material for intermediate temperature SOFCs.

  6. Targeted Inhibition of the miR-199a/214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Condition

    Science.gov (United States)

    Xu, Xuehu; An, Xiuli; Wang, Shu

    2016-01-01

    The human induced pluripotent stem cell (hiPSC) provides a breakthrough approach that helps overcoming ethical and allergenic challenges posed in application of neural stem cells (NSCs) in targeted cancer gene therapy. However, the tumor-tropic capacity of hiPSC-derived NSCs (hiPS-NSCs) still has much room to improve. Here we attempted to promote the tumor tropism of hiPS-NSCs by manipulating the activity of endogenous miR-199a/214 cluster that is involved in regulation of hypoxia-stimulated cell migration. We first developed a baculovirus-delivered CRISPR interference (CRISPRi) system that sterically blocked the E-box element in the promoter of the miR-199a/214 cluster with an RNA-guided catalytically dead Cas9 (dCas9). We then applied this CRISPRi system to hiPS-NSCs and successfully suppressed the expression of miR-199a-5p, miR-199a-3p, and miR-214 in the microRNA gene cluster. Meanwhile, the expression levels of their targets related to regulation of hypoxia-stimulated cell migration, such as HIF1A, MET, and MAPK1, were upregulated. Further migration assays demonstrated that the targeted inhibition of the miR-199a/214 cluster significantly enhanced the tumor tropism of hiPS-NSCs both in vitro and in vivo. These findings suggest a novel application of CRISPRi in NSC-based tumor-targeted gene therapy. PMID:27965712

  7. Targeted Inhibition of the miR-199a/214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Condition

    Directory of Open Access Journals (Sweden)

    Yumei Luo

    2016-01-01

    Full Text Available The human induced pluripotent stem cell (hiPSC provides a breakthrough approach that helps overcoming ethical and allergenic challenges posed in application of neural stem cells (NSCs in targeted cancer gene therapy. However, the tumor-tropic capacity of hiPSC-derived NSCs (hiPS-NSCs still has much room to improve. Here we attempted to promote the tumor tropism of hiPS-NSCs by manipulating the activity of endogenous miR-199a/214 cluster that is involved in regulation of hypoxia-stimulated cell migration. We first developed a baculovirus-delivered CRISPR interference (CRISPRi system that sterically blocked the E-box element in the promoter of the miR-199a/214 cluster with an RNA-guided catalytically dead Cas9 (dCas9. We then applied this CRISPRi system to hiPS-NSCs and successfully suppressed the expression of miR-199a-5p, miR-199a-3p, and miR-214 in the microRNA gene cluster. Meanwhile, the expression levels of their targets related to regulation of hypoxia-stimulated cell migration, such as HIF1A, MET, and MAPK1, were upregulated. Further migration assays demonstrated that the targeted inhibition of the miR-199a/214 cluster significantly enhanced the tumor tropism of hiPS-NSCs both in vitro and in vivo. These findings suggest a novel application of CRISPRi in NSC-based tumor-targeted gene therapy.

  8. Corrective Action Investigation Plan for Corrective Action Unit 214: Bunkers and Storage Areas Nevada Test Site, Nevada: Revision 0, Including Record of Technical Change No. 1 and No. 2

    Energy Technology Data Exchange (ETDEWEB)

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2003-05-16

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 214 under the Federal Facility Agreement and Consent Order. Located in Areas 5, 11, and 25 of the Nevada Test Site, CAU 214 consists of nine Corrective Action Sites (CASs): 05-99-01, Fallout Shelters; 11-22-03, Drum; 25-99-12, Fly Ash Storage; 25-23-01, Contaminated Materials; 25-23-19, Radioactive Material Storage; 25-99-18, Storage Area; 25-34-03, Motor Dr/Gr Assembly (Bunker); 25-34-04, Motor Dr/Gr Assembly (Bunker); and 25-34-05, Motor Dr/Gr Assembly (Bunker). These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). The suspected contaminants and critical analyte s for CAU 214 include oil (total petroleum hydrocarbons-diesel-range organics [TPH-DRO], polychlorinated biphenyls [PCBs]), pesticides (chlordane, heptachlor, 4,4-DDT), barium, cadmium, chronium, lubricants (TPH-DRO, TPH-gasoline-range organics [GRO]), and fly ash (arsenic). The land-use zones where CAU 214 CASs are located dictate that future land uses will be limited to nonresidential (i.e., industrial) activities. The results of this field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the corrective action decision document.

  9. Kinetic study of benzyl [1-14C]acetate as a potential probe for astrocytic energy metabolism in the rat brain: Comparison with benzyl [2-14C]acetate.

    Science.gov (United States)

    Okada, Maki; Yanamoto, Kazuhiko; Kagawa, Tomohiko; Yoshino, Keiko; Hosoi, Rie; Abe, Kohji; Zhang, Ming-Rong; Inoue, Osamu

    2016-02-01

    Brain uptake of [(14)C]acetate has been reported to be a useful marker of astrocytic energy metabolism. In addition to uptake values, the rate of radiolabeled acetate washout from the brain appears to reflect CO2 exhaustion and oxygen consumption in astrocytes. We measured the time-radioactivity curves of benzyl [1-(14)C]acetate ([1-(14)C]BA), a lipophilic probe of [1-(14)C]acetate, and compared it with that of benzyl [2-(14)C]acetate ([2-(14)C]BA) in rat brains. The highest brain uptake was observed immediately after injecting either [1-(14)C]BA or [2-(14)C]BA, and both subsequently disappeared from the brain in a single-exponential manner. Estimated [1-(14)C]BA washout rates in the cerebral cortex and cerebellum were higher than those of [2-(14)C]BA. These results suggested that [1-(14)C]BA could be a useful probe for estimating the astrocytic oxidative metabolism. The [1-(14)C]BA washout rate in the cerebral cortex of immature rats was lower than that of mature rats. An autoradiographic study showed that the washout rates of [1-(14)C]BA from the rat brains of a lithium-pilocarpine-induced status epilepticus model were not significantly different from the values in control rat brains except for the medial septal nucleus. These results implied that the enhancement of amino acid turnover rate rather than astrocytic oxidative metabolism was increased in status epilepticus. © The Author(s) 2015.

  10. Tracer studies on the incorporation of [2-14C]-DL-mevalonate into chlorophylls a and b, alpha-chaconine, and alpha-solanine of potato sprouts.

    Science.gov (United States)

    Kozukue, N; Tsuchida, H; Friedman, M

    2001-01-01

    Chlorophyll and glycoalkaloids are synthesized in different parts of the potato plant including leaves, tubers, and sprouts. Although light stimulates the biosynthesis of both constituents, the question of whether the two biosynthetic pathways are under the same genetic control has not been resolved. This study investigated the dynamics of incorporation of labeled [2-(14)C]-DL-mavalonate into chlorophyll a, chlorophyll b, and the glycoalkaloids alpha-chaconine and alpha-solanine in potato sprouts after 7 and 14 days of storage in the light and in the dark. No chlorophyll synthesis occurred in the dark. Fractionation of the "glycoalkaloid" extract followed by high-performance liquid chromatography produced four peaks. The fractions were collected and analyzed for radioactivity. About 80% of the radioactivity resided in fraction 1, the composition of which is unknown. Two of the fractions, with 1-14% of the original label, were alpha-chaconine and alpha-solanine. The radioactivity derived from mevalonate largely resides in unidentified compound(s) eluting as a single peak on the HPLC column before the peaks associated with the glycoalkaloids. The specific radioactivity of alpha-chaconine and alpha-solanine increased approximately 2-fold in going from 7 to 14 days of exposure in the light and in the dark. These and additional observations point to the near identity of the dynamics of biosynthesis of the two glycoalkaloids. These data also implicate a non-mevalonate pathway for the synthesis of both chlorophylls and the glycoalkaloids and are consistent with independent genetic control of the concurrent formation of the two classes of compounds during greening of potatoes.

  11. A Piscine Birnavirus Induces Inhibition of Protein Synthesis in CHSE-214 Cells Primarily through the Induction of eIF2α Phosphorylation

    Directory of Open Access Journals (Sweden)

    Amr A.A. Gamil

    2015-04-01

    Full Text Available Inhibition of protein synthesis represents one of the antiviral mechanisms employed by cells and it is also used by viruses for their own propagation. To what extent members of the Birnaviridae family employ such strategies is not well understood. Here we use a type-strain of the Aquabirnavirus, infectious pancreatic necrosis virus (IPNV, to investigate this phenomenon in vitro. CHSE-214 cells were infected with IPNV and at 3, 12, 24, and 48 hours post infection (hpi before the cells were harvested and labeled with S35 methionine to assess protein synthesis. eIF2α phosphorylation was examined by Western blot while RT-qPCR was used to assess virus replication and the expression levels of IFN-α, Mx1 and PKR. Cellular responses to IPNV infection were assessed by DNA laddering, Caspase-3 assays and flow cytometry. The results show that the onset and kinetics of eIF2α phosphorylation was similar to that of protein synthesis inhibition as shown by metabolic labeling. Increased virus replication and virus protein formation was observed by 12 hpi, peaking at 24 hpi. Apoptosis was induced in a small fraction (1−2% of IPNV-infected CHSE cells from 24 hpi while necrotic/late apoptotic cells increased from 10% by 24 hpi to 59% at 48 hpi, as shown by flow cytometry. These results were in accordance with a small decline in cell viability by 24hpi, dropping below 50% by 48 hpi. IPNV induced IFN-α mRNA upregulation by 24 hpi while no change was observed in the expression of Mx1 and PKR mRNA. Collectively, these findings show that IPNV induces inhibition of protein synthesis in CHSE cells through phosphorylation of eIF2α with minimal involvement of apoptosis. The anticipation is that protein inhibition is used by the virus to evade the host innate antiviral responses.

  12. Sõpruse pst. 214 / Ilme Trepp

    Index Scriptorium Estoniae

    Trepp, Ilme

    2002-01-01

    Rööptekst vene k. lk. 33-34. Ühistute huvide eest seismiseks loodi Mustamäe Korteriühistute Nõukoda, mis seab eesmärgiks esindada Mustamäe korteriühistuid, teha koostööd linnavalitsusega, vahetada infot ja kasulikke kogemusi

  13. 21.4.Mental deficiency

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930413 Clinlcal study of mental disorders inpost—stroke patients.WANG Huanlin (王焕林),et al.102nd Hosp,PLA Changzhou,213003.Chin J Nerv & Ment Dis 1992;18(5):281—283.The mental disorder has been studied bymeans of DSM-Ⅱ diagnostic criteria in 104hospitalized stroke patients.The results re-vealed that the prevalence of mental disorderwas 46.2% in post-stroke patients.Theprevalence of poststroke depression (PSD),de-pressive neurosis (DN),multiple infarct demen-tia (MID),and post—stroke mania (PSM) were22.1%,11.5%,8.7%,and 3(?)8% respectively.It was indicated that the lesions of frontal cortexand left basal ganglia were strongly associated

  14. 21.4.Mental deficiency

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    920201 Assessment of Hasegawa’s demen-tia scale for screening and diagnosing de-mentia in the elderly.GAO Zhixu (高之旭),etal.Shanghai Ment Health Center,Shanghai,200030.Chin J Neurol & Psychiat 1991; 24 (5):258-261.A sample of 2573 elder inhabitants in Shanghai

  15. QM/MM free-energy simulations of reaction in Serratia marcescens Chitinase B reveal the protonation state of Asp142 and the critical role of Tyr214.

    Science.gov (United States)

    Jitonnom, Jitrayut; Limb, Michael A L; Mulholland, Adrian J

    2014-05-01

    Serratia marcescens Chitinase B (ChiB), belonging to the glycosidase family 18 (GH18), catalyzes the hydrolysis of β-1,4-glycosidic bond, with retention of configuration, via an unusual substrate-assisted mechanism, in which the substrate itself acts as an intramolecular nucleophile. Here, both elementary steps (glycosylation and deglycosylation) of the ChiB-catalyzed reaction are investigated by means of combined quantum mechanics/molecular mechanics (QM/MM) umbrella sampling molecular dynamics (MD) simulations at the SCC-DFTB/CHARMM22 level of theory. We examine the influence of the Asp142 protonation state on the reaction and the role that this residue performs in the reaction. Our simulations show that reaction with a neutral Asp142 is preferred and demonstrate that this residue provides electrostatic stabilization of the oxazolinium ion intermediate formed in the reaction. Insight into the conformational itinerary ((1,4)B↔(4)H5↔(4)C1) adopted by the substrate (bound in subsite -1) along the preferred reaction pathway is also provided by the simulations. The relative energies of the stationary points found along the reaction pathway calculated with SCC-DFTB and B3LYP were compared. The results suggest that SCC-DFTB is an accurate method for estimating the relative barriers for both steps of the reaction; however, it was found to overestimate the relative energy of an intermediate formed in the reaction when compared with the higher level of theory. Glycosylation is suggested to be a rate-determining step in the reaction with calculated overall reaction free-energy barrier of 20.5 kcal/mol, in a reasonable agreement with the 16.1 kcal/mol barrier derived from the experiment. The role of Tyr214 in catalysis was also investigated with the results, indicating that the residue plays a critical role in the deglycosylation step of the reaction. Simulations of the enzyme-product complex were also performed with an unbinding event suggested to have been observed

  16. Isotope shifts of the 6d{sup 2} D{sub 3/2}-7 p{sup 2} P{sub 1/2} transition in trapped short-lived {sup 209-214}Ra{sup +}

    Energy Technology Data Exchange (ETDEWEB)

    Giri, G. S.; Versolato, O. O.; Berg, J. E. van den; Boell, O.; Dammalapati, U.; Hoek, D. J. van der; Jungmann, K.; Kruithof, W. L.; Mueller, S.; Nunez Portela, M.; Onderwater, C. J. G.; Santra, B.; Timmermans, R. G. E.; Wansbeek, L. W.; Willmann, L.; Wilschut, H. W. [University of Groningen, Kernfysisch Versneller Instituut, Groningen NL-9747 AA (Netherlands)

    2011-08-15

    Laser spectroscopy of short-lived radium isotopes in a linear Paul trap has been performed. The isotope shifts of the 6d{sup 2} D{sub 3/2} -7 p{sup 2} P{sub 1/2} transition in {sup 209-214}Ra{sup +}, which are sensitive to the short-range part of the atomic wave functions, were measured. The results are essential experimental input for improving the precision of atomic structure calculations. This is indispensable for parity violation in Ra{sup +} aiming at the determination of the weak mixing angle.

  17. 214例黄疸原因待查患者临床表现及肝穿病理特点分析%Analysis of 214 cases of clinical manifestation and histopathological features in percutaneous liver biopsy for the icteric with unrecognized reasons

    Institute of Scientific and Technical Information of China (English)

    龙江; 王华光; 谭永康

    2007-01-01

    目的 研究214例入院时病毒学指标是阴性的黄疸原因待查患者的临床表现和肝穿病理特点.方法 选择病毒学指标阴性的黄疸待查患者,将他们的症状、体征、肝功能、免疫学以及血常规依据病种分列成表,并加以分析.结果 病毒性肝炎、药物性肝病、自身免疫性肝病、梗阻性黄疸、原因不明黄疸、暴发性肝衰竭以及妊娠急性脂肪肝患者的不适主诉较多(>60%).PBC、酒精性肝病、原因不明黄疸和暴发性肝衰竭患者的阳性体征较多(>60%).病毒性肝炎、药物性肝病、原因不明黄疸、暴发性肝衰竭以及妊娠急性脂肪肝患者的肝功能明显异常.病毒性肝炎、酒精性肝病、肝豆状核变性、胰腺癌和妊娠急性脂肪肝患者的血沉值明显升高,AFP的明显升高仅见于肝癌患者.结论 依据疾病出现的频率,从高到低依次是自身免疫性肝病、病毒性肝炎、药物性肝病、梗阻性黄疸、酒精性肝病、先天性疾病、不明原因黄疸.除梗阻性黄疸有陶土样便以外,消化系统常见的不适症状多数是非特异的.年龄和性别有时是重要的因素.肝穿活检是一项重要的检查,超声、CT、肝血管造影、ERCP(逆行胆胰管造影)同样是必要的检查.%[Objective] To study the clinical and histopathological speciality of 214 icteric patients whose viral indexes were negative when admitted to the hospital. [Methods] 214 patients with jaundice whose viral indexes were negative were selected. We both wrote down and analyzed their symptoms and physical signs, results of liver function, immunodetection and blood routine. [Results] The patients suffered from these diseases including viral hepatitis, the drug-induced liver disease, autoimmune liver disease, obstructive jaundice, unknown reason, acute liver failure, cholestatic hepatitis and acute adiposis hepatica on gestational period who have more symptoms than other diseases( > 60

  18. Studying Low Mass X-Ray Binaries: Revealing the Optical Counterpart in 1747-214 and Measuring the Masses of the Black Holes in 1859+226 and 1009-45

    Science.gov (United States)

    Gelino, Dawn M.; Tomsick, John A.

    2003-02-01

    Low mass x-ray binaries (LMXBs) contain compact, black hole (BH) or neutron star (NS) primaries, and cool, low-mass secondary stars. A limited number of BHs and NSs have accurate mass measurements. It is important to determine the primary mass of the LMXBs to better understand how BH masses influence their outburst behavior, and to better constrain the NS equations of state. To determine the mass of the primary object we need to measure the orbital inclination, i. We propose to determine i for two BH LMXBs, XTE J1859+226 and GRS 1009-45 (=N Vel 93) through modeling of their ellipsoidal variations. Because most LMXBs are not eclipsing, modeling their light curves is currently the only feasible method for determining the inclination. We will model the light curves with WD98. We also propose to identify the optical counterpart to the NS system EXO 1747-214, in order to begin the process of measuring the NS mass. We have successfully used NOAO facilities and this modeling technique to find accurate BH masses in four LMXBs. In order to expand the sample of known BH and NS systems, we request seven nights on the KPNO and CTIO 4m to obtain optical and infrared data on XTE J1859+226, GRS 1009-45, and EXO 1747-214.

  19. [Oxidation of [1-14C]palmitoyl-CoA, [1-14C]acetyl-CoA and [2-14C]pyruvate in rabbit adrenal, liver and heart mitochondria under normal conditions and in acute stress].

    Science.gov (United States)

    Mandrik, K A; Doroshkevich, N A; Vinogradov, V V

    1986-01-01

    The acute immobilized stress was studied for its effect on oxidation rate of [1-14C]palmitoyl-CoA, [1-14C]acetyl-CoA and [2-14C]pyruvate in mitochondria of the adrenals, liver and heart of rabbits. The stress effect on the energy metabolism of adrenals is associated with an increase of the rate of CO2 formation from pyruvate and with a decrease of the rate of CO2 formation from palmitoyl-CoA. Intensified oxidation of all substrates is observed in the heart mitochondria. The processes of beta-oxidation are more active in the liver. The data obtained evidence for differences in the mechanisms of energy metabolism reconstruction under acute stress in tissues with different functional specialization.

  20. Active-site peptides of acetylcholinesterase of Electrophorus electricus: labelling of His-440 by 1-bromo-[2-14C]pinacolone and Ser-200 by tritiated diisopropyl fluorophosphate.

    Science.gov (United States)

    Salih, E; Chishti, S B; Vicedomine, P; Cohen, S G; Chiara, D C; Cohen, J B

    1994-10-19

    To characterize the structure of the active site of acetylcholinesterase (AChE) from the electric organ of E. electricus, we identified sites of incorporation of two active-site affinity labels, [3H]diisopropyl fluorophosphate ([3H]DFP), and 1-bromo-2-[14C]pinacolone ([14C]BrPin). AChE was isolated, purified, inactivated and digested with trypsin, and peptides containing 3H or 14C were purified by reverse-phase HPLC and characterized by N-terminal sequence analysis. [3H]DFP, labelling Ser-200, was found in a single peptide, QVTIFGESAGAASVGMHLLSPDSR, 83% identical with the sequence from Thr-193 to Arg-216 deduced for AChE of T. californica, with Gln, Ala, Leu, and Asp in place of Thr-193, Gly-203, Ile-210 and Gly-214, respectively, and 87% identical with that from bovine and human brain AChEs. Inactivation by [14C]BrPin led to two radioactive peptides. One, ASNLVWPEWMGVIHGYEIEFVFGLPLEK, was 96% identical with that extending from Ala-427 to Lys-454 of T. californica. Release of 14C in cycle 14 established reaction of [14C]BrPin with active-site His-440, protected by 5-trimethylammonio-2-pentanone (TAP). The other peptide, LLXVTENIDDAER, 77% homologous with that of T. californica extending from Leu-531 to Arg-543, had label associated with the third cycle, not protected by TAP, corresponding to Asn-533. The slow inactivation of eel AChE by reaction of [14C]BrPin at His-440 contrasts with that of AChE from T. nobiliana, where it reacts rapidly with a free cysteine, Cys-231, not present in eel AChE. For both AChEs, inactivation by BrPin prevents subsequent reaction with [3H]DFP, and prior inactivation by DFP does not prevent reactions with [14C]BrPin.

  1. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228

    Energy Technology Data Exchange (ETDEWEB)

    Aleissa, Khalid A.; Almasoud, Fahad I.; Islam, Mohammed S. [Atomic Energy Research Institute, King Abdul Aziz City for Science and Technology, P.O. Box 6086, Riyadh 11442 (Saudi Arabia); L' Annunziata, Michael F. [IAEA Expert, Montague Group, P.O. Box 5033, Oceanside, CA 92052-5033 (United States)], E-mail: mlannunziata@cox.net

    2008-12-15

    The activities of {sup 228}Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide {sup 228}Ac. The radium was pre-concentrated on MnO{sub 2} and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter {sup 228}Ra({sup 228}Ac), the daughter nuclide {sup 228}Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by {sup 228}Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9{+-}0.1% was measured for {sup 228}Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317{+-}0.013 cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1 g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of {sup 228}Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for {sup 228}Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure {sup 228}Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  2. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228.

    Science.gov (United States)

    Aleissa, Khalid A; Almasoud, Fahad I; Islam, Mohammed S; L'Annunziata, Michael F

    2008-12-01

    The activities of (228)Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide (228)Ac. The radium was pre-concentrated on MnO(2) and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter (228)Ra((228)Ac), the daughter nuclide (228)Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by (228)Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9+/-0.1% was measured for (228)Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317+/-0.013cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of (228)Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for (228)Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure (228)Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  3. Purification of selenium from thorium, uranium, radium, actinium and potassium impurities for low background measurements

    Energy Technology Data Exchange (ETDEWEB)

    Rakhimov, A.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz); Warot, G. [CEA-CNRS, Modane (France). Laboratoire Souterrain de Modane (LSM); Karaivanov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Institute for Nuclear Research and Nuclear Energy (INRNE), Sofia (Bulgaria); Kochetov, O.I.; Lebedev, N.A.; Filosofov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Mukhamedshina, N.M.; Sadikov, I.I. [Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz)

    2013-07-01

    A technique of selenium purification from {sup 232}Th, {sup 238}U, {sup 226,228}Ra, {sup 227}Ac and {sup 40}K was developed. This technique is simple to perform and employs a minimum number of highly pure reagents (bidistilled water, nitric acid). Operations carried out during purification (elution, evaporation) practically exclude losses of the target product (chemical yields of Se > 99%). A test purification of 100 g of selenium was carried out using this technique. The efficiency of this technique was confirmed by low background gamma spectrometry of the purified selenium sample. Distribution coefficients of Th, U, Ra and Ac on DOWEX 50W- x 8 cation-exchange resin at different concentrations of selenium and nitric acid were experimentally determinated. Instrumental neutron activation analysis of bidistilled water, deionized water and nitric acid was performed. (orig.)

  4. The release of dissolved actinium to the ocean: A global comparison of different end-members

    Science.gov (United States)

    Geibert, W.; Charette, M.; Kim, G.; Moore, W.S.; Street, J.; Young, M.; Paytan, A.

    2008-01-01

    The measurement of short-lived 223Ra often involves a second measurement for supported activities, which represents 227Ac in the sample. Here we exploit this fact, presenting a set of 284 values on the oceanic distribution of 227Ac, which was collected when analyzing water samples for short-lived radium isotopes by the radium delayed coincidence counting system. The present work compiles 227Ac data from coastal regions all over the northern hemisphere, including values from ground water, from estuaries and lagoons, and from marine end-members. Deep-sea samples from a continental slope off Puerto Rico and from an active vent site near Hawaii complete the overview of 227Ac near its potential sources. The average 227Ac activities of nearshore marine end-members range from 0.4??dpm m- 3 at the Gulf of Mexico to 3.0??dpm m- 3 in the coastal waters of the Korean Strait. In analogy to 228Ra, we find the extension of adjacent shelf regions to play a substantial role for 227Ac activities, although less pronounced than for radium, due to its weaker shelf source. Based on previously published values, we calculate an open ocean 227Ac inventory of 1.35 * 1018??dpm 227Acex in the ocean, which corresponds to 37??moles, or 8.4??kg. This implies a flux of 127??dpm m-2 y- 1 from the deep-sea floor. For the shelf regions, we obtain a global inventory of 227Ac of 4.5 * 1015??dpm, which cannot be converted directly into a flux value, as the regional loss term of 227Ac to the open ocean would have to be included. Ac has so far been considered to behave similarly to Ra in the marine environment, with the exception of a strong Ac source in the deep-sea due to 231Paex. Here, we present evidence of geochemical differences between Ac, which is retained in a warm vent system, and Ra, which is readily released [Moore, W.S., Ussler, W. and Paull, C.K., 2008-this issue. Short-lived radium isotopes in the Hawaiian margin: Evidence for large fluid fluxes through the Puna Ridge. Marine Chemistry]. Another potential mechanism of producing deviations in 227Ac/228Ra and daughter isotope ratios from the expected production value of lithogenic material is observed at reducing environments, where enrichment in uranium may occur. The presented data here may serve as a reference for including 227Ac in circulation models, and the overview provides values for some end-members that contribute to the global Ac distribution. ?? 2007 Elsevier B.V. All rights reserved.

  5. Rafael Mérida Jiménez, Transmisión y difusión de la literatura caballeresca. Doce estudios de recepción cultural hispánica (siglos XIII-XVII), Lleida, Edicions de la Universitat de Lleida,2013. 214 pp.

    OpenAIRE

    Neri, Stefano

    2013-01-01

    Reseña del libro: Rafael Mérida Jiménez, Transmisión y difusión de la literatura caballeresca. Doce estudios de recepción cultural hispánica (siglos XIII-XVII), Lleida, Edicions de la Universitat de Lleida,2013. 214 pp.

  6. Short and mid-term effects of artificial defoliation on the growth of euramerican poplar (Populus × canadensis clone I-214 in poplar stands in relation to the intensity and seasonal timing of defoliation.

    Directory of Open Access Journals (Sweden)

    Allegro G

    2017-08-01

    Full Text Available The impact of artificial defoliations on the growth of euramerican poplar (Populus × canadensis clone I-214 was investigated in three field assays. Young poplar trees (1st-3rd cultivation year were subjected to 25%, 50%, 75% and 100% defoliation either in the early or in the late growing season (or in both periods, and their growth was measured in the same year and during the following 2-3 years. Only trees treated to 75% and 100% defoliation exhibited a significant growth decrease compared to control trees: the diametric growth of early defoliated trees was reduced up to 20-40% in the year of defoliation, whereas trees defoliated at the same rates late in the season, or defoliated twice in a single year, suffered the heaviest damage, showing a growth loss up to 50% in the year following the defoliation events. In the latter cases, a delayed sprouting at spring and the death of branchlets were observed. A financial analysis showed that the economic damage caused by the highest defoliation rates, mainly when occurring late in the season, definitely exceeds the cost of treatments, thus suggesting the adoption of appropriate control strategies.

  7. A recombinant CHSE-214 cell line expressing an Mx1 promoter-reporter system responds to both interferon type I and type II from salmonids and represents a versatile tool to study the IFN-system in teleost fish.

    Science.gov (United States)

    Jørgensen, Jorunn B; Johansen, Audny; Hegseth, Marit N; Zou, Jun; Robertsen, Børre; Collet, Bertrand; Secombes, Christopher J

    2007-12-01

    A transgenic cell line for the detection of salmon interferons (IFNs) has been established. It is based on a CHSE-214 cell line containing a reporter construct expressing firefly luciferase under the control of the rainbow trout promoter for the IFN-induced Mx1 gene. This cell line, named CHSE-Mx10, showed IFN-induced luciferase expression after more than 80 passages, confirming the stability of this cell line. Interestingly, the Mx promoter was shown to respond to both salmon IFN-alpha/beta and trout IFN-gamma in a dose-dependent manner, while there was no response to TNF-alpha and IL-1beta. IFN-alpha/beta activity could be measured at a range of 9-150 U/ml, and IFN-gamma showed activity between 10 and 100 ng/ml. The reproducibility of both responses was good. The CHSE-Mx10 reporter system constitutes a versatile tool to study the induction and regulation of IFN signaling in teleost fish. A preliminary study presented herein suggests that both infectious pancreas necrosis virus (IPNV) and salmon pancreas disease virus (SPDV) may block activation of the Mx promoter in CHSE-Mx10 stimulated with IFN-alpha/beta.

  8. Paleomagnetism of Basaltic Lava Flows in Coreholes ICPP 213, ICPP-214, ICPP-215, and USGS 128 Near the Vadose Zone Research Park, Idaho Nuclear Technology and Engineering Center, Idaho National Engineering and Environmental Laboratory, Idaho

    Science.gov (United States)

    Champion, Duane E.; Herman, Theodore C.

    2003-01-01

    A paleomagnetic study was conducted on basalt from 41 lava flows represented in about 2,300 ft of core from coreholes ICPP-213, ICPP-214, ICPP-215, and USGS 128. These wells are in the area of the Idaho Nuclear Technology and Engineering Center (INTEC) Vadose Zone Research Park within the Idaho National Engineering and Environmental Laboratory (INEEL). Paleomagnetic measurements were made on 508 samples from the four coreholes, which are compared to each other, and to surface outcrop paleomagnetic data. In general, subhorizontal lines of correlation exist between sediment layers and between basalt layers in the area of the new percolation ponds. Some of the basalt flows and flow sequences are strongly correlative at different depth intervals and represent important stratigraphic unifying elements. Some units pinch out, or thicken or thin even over short separation distances of about 1,500 ft. A more distant correlation of more than 1 mile to corehole USGS 128 is possible for several of the basalt flows, but at greater depth. This is probably due to the broad subsidence of the eastern Snake River Plain centered along its topographic axis located to the south of INEEL. This study shows this most clearly in the oldest portions of the cored sections that have differentially subsided the greatest amount.

  9. 肿瘤型膝关节假体术后并发症214例分析%Complications after the reconstruction of bone defects in the knee with tumor endoprostheses

    Institute of Scientific and Technical Information of China (English)

    覃澍; 董扬

    2016-01-01

    Objective To retrospectively analyze the prosthesis survival and complications after custom-made tumor prosthesis replacement of the knee joint. Methods Data of 214 patients with bone tumors around knee joints who underwent custom-prosthetic replacement from January 2003 to January 2013, were retrospectively analyzed. The diagnoses were osteosarcoma ( n = 136 ), giant cell tumor ( n = 49 ), aneurysmal bone cyst ( n = 1 ), fasciculated sarcoma ( n = 3 ), malignant fibrous histiocytoma ( n = 10 ), chondrosarcoma ( n = 6 ), chondroblastoma ( n = 1 ), metastatic tumor ( n = 4 ) and other types of bone tumors ( n = 3 ). The distal femur was affected in 150 patients and the proximal tibia in 64. Results Totally 187 patients were followed up for a mean period of 47 months ( range: 16 -122 months ). Complications occurred in 47 patients: early stage infection or nonunion of wounds ( n = 15 ), peroneal palsy ( n = 3 ), late infection ( n = 5 ), aseptic loosening ( n = 15 ), breakage of the prosthesis ( n = 4 ) and periprosthetic fracture ( n = 5 ). Conclusions The complication rate of proximal tibia tumor is higher than the rate of the distal femur. Common complications of tumor prosthesis replacement of the knee joint are: aseptic loosening, infection or nonunion of wounds, peroneal palsy, late infection, aseptic loosening, breakage of prosthesis and periprosthetic fracture. Common complications of tumor prosthesis replacement of the knee joint can be treated and prevented.%目的:分析肿瘤型膝关节假体置换术后并发症的发生情况以及处理方式。方法回顾性分析2003年1月至2012年1月,在我院行膝关节假体置换的214例术后并发症发生情况;其中骨肉瘤136例,骨巨细胞瘤49例,动脉瘤样股囊肿1例,梭形细胞肉瘤3例,恶性纤维组织细胞瘤10例,软骨肉瘤6例,软骨母细胞瘤1例,软骨细胞瘤1例,转移癌4例,其它骨肿瘤3例;根据位置分类,发生于股骨远端150

  10. The effects of height and BMI on prostate cancer incidence and mortality: a Mendelian randomization study in 20,848 cases and 20,214 controls from the PRACTICAL consortium.

    Science.gov (United States)

    Davies, Neil M; Gaunt, Tom R; Lewis, Sarah J; Holly, Jeff; Donovan, Jenny L; Hamdy, Freddie C; Kemp, John P; Eeles, Rosalind; Easton, Doug; Kote-Jarai, Zsofia; Al Olama, Ali Amin; Benlloch, Sara; Muir, Kenneth; Giles, Graham G; Wiklund, Fredrik; Gronberg, Henrik; Haiman, Christopher A; Schleutker, Johanna; Nordestgaard, Børge G; Travis, Ruth C; Neal, David; Pashayan, Nora; Khaw, Kay-Tee; Stanford, Janet L; Blot, William J; Thibodeau, Stephen; Maier, Christiane; Kibel, Adam S; Cybulski, Cezary; Cannon-Albright, Lisa; Brenner, Hermann; Park, Jong; Kaneva, Radka; Batra, Jyotsna; Teixeira, Manuel R; Pandha, Hardev; Lathrop, Mark; Smith, George Davey; Martin, Richard M

    2015-11-01

    Epidemiological studies suggest a potential role for obesity and determinants of adult stature in prostate cancer risk and mortality, but the relationships described in the literature are complex. To address uncertainty over the causal nature of previous observational findings, we investigated associations of height- and adiposity-related genetic variants with prostate cancer risk and mortality. We conducted a case-control study based on 20,848 prostate cancers and 20,214 controls of European ancestry from 22 studies in the PRACTICAL consortium. We constructed genetic risk scores that summed each man's number of height and BMI increasing alleles across multiple single nucleotide polymorphisms robustly associated with each phenotype from published genome-wide association studies. The genetic risk scores explained 6.31 and 1.46% of the variability in height and BMI, respectively. There was only weak evidence that genetic variants previously associated with increased BMI were associated with a lower prostate cancer risk (odds ratio per standard deviation increase in BMI genetic score 0.98; 95% CI 0.96, 1.00; p = 0.07). Genetic variants associated with increased height were not associated with prostate cancer incidence (OR 0.99; 95% CI 0.97, 1.01; p = 0.23), but were associated with an increase (OR 1.13; 95 % CI 1.08, 1.20) in prostate cancer mortality among low-grade disease (p heterogeneity, low vs. high grade prostate cancer risk, suggesting that previously reported observational associations may reflect common environmental determinants of height or BMI and prostate cancer risk. Genetically elevated height and BMI were associated with increased mortality (prostate cancer-specific and all-cause, respectively) in men with low-grade disease, a potentially informative but novel finding that requires replication.

  11. 49 CFR 214.109 - Scaffolding.

    Science.gov (United States)

    2010-10-01

    ... unit does not meet this requirement, suitable outrigger frames shall be employed to achieve this least base dimension, or equivalent provisions shall be made to guy or brace the unit against tipping....

  12. Fungal Planet description sheets: 214–280

    NARCIS (Netherlands)

    Crous, P.W.; Shivas, R.G.; Quaedvlieg, W.; Bank, van der M.; Zhang, Y.; Summerell, B.A.; Guarro, J.; Wingfield, M.J.; Wood, A.R.; Alfenas, A.C.; Braun, U.; Cano-Lira, J.F.; Garcia, D.; Marin-Felix, Y.; Alvarado, P.; Andrade, J.P.; Armengol, J.; Assefa, A.; Breeÿen, den A.; Camele, I.; Cheewangkoon, R.; Souza, De J.T.; Duong, T.A.; Esteve-Raventós, F.; Fournier, J.; Frisullo, S.; García-Jiménez, J.; Gardiennet, A.; Gené, J.; Hernández-Restrepo, M.; Hirooka, Y.; Hospenthal, D.R.; King, A.; Lechat, C.; Lombard, L.; Mang, S.M.; Marbach, P.A.S.; Marincowitz, S.; Montaño-Mata, N.J.; Moreno, G.; Perez, C.A.; Pérez Sierra, A.M.; Robertson, J.L.; Roux, J.; Rubio, E.; Schumacher, R.K.; Stchigel, A.M.; Sutton, D.A.; Tan, Y.P.; Thompson, E.H.; Vanderlinde, E.; Walker, A.K.; Walker, D.M.; Wickes, B.L.; Wong, P.T.W.; Groenewald, J.Z.

    2014-01-01

    Novel species of microfungi described in the present study include the following from South Africa: Cercosporella dolichandrae from Dolichandra unguiscati, Seiridium podocarpi from Podocarpus latifolius, Pseudocercospora parapseudarthriae from Pseudarthria hookeri, Neodevriesia coryneliae from

  13. 49 CFR 214.113 - Head protection.

    Science.gov (United States)

    2010-10-01

    ... protection. (a) Railroad bridge workers working in areas where there is a possible danger of head injury from... approved by the Director of the Federal Register in accordance with 5 U.S.C. 552(a) and 1 CFR part 51... Avenue, SE., Washington, DC, or at the National Archives and Records Administration (NARA)....

  14. Proclamation No. 214, 3 February 1988.

    Science.gov (United States)

    1988-01-01

    This Proclamation declares the second week of February 1988 and every year thereafter as Respect and Care for Life Week. The text of the Proclamation, including its preamble, is as follows: "Whereas, the UN Declaration on Rights of the Child provides that 'the Child, by reason of his physical and mental immaturity, needs special safeguards and care, including appropriate legal protection, before as well as after birth'; Whereas, Section 12, Article 2 of the Constitution provides that it is the policy of the State to 'protect the life of the mother and the life of the unborn from conception'; Whereas, available statistics detail the frightening losses of innocent human lives by abortion; and Whereas, in pursuance of the above constitutional mandate and in support of the UN universal declaration and in order to instill the same, as well as the value and sacredness of human life, in the minds and hearts of the Filipino people and thereby help reverse the above statistical trend, it becomes imperative to set aside a period of time for them to ponder and focus attention on their moral and constitutional obligations to protect human life or one's inherent right to life; Now, Therefore, I, Corazon C. Aquino, President of the Philippines, by virtue of the powers vested in me by law, do hereby declare the second week of February 1988 and every year thereafter as "Respect and Care for Life Week" under the auspices of the Department of Social Welfare and Development and Pro-Life Philippines, and other involved entities or organization[s]." full text

  15. 24 CFR 214.3 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... mortgage counseling, all counseling shall involve the creation of an action plan. Education. Formal classes, with established curriculum and instructional goals provided in a group or classroom setting,...

  16. QTL Information Table: 214 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available n of an Elite Rice Hybrid Revealed That Heterozygotes Are Not Always Advantageous for Performance. Genetics 162, 1885-1895. http://www.genetics.org/cgi/content/abstract/162/4/1885 ...

  17. 49 CFR 214.7 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... not limited to, rope grabs, ripstitch lanyards, specially woven lanyards, tearing or deforming... notification of non-complying conditions on on-track roadway maintenance machines and hi-rail vehicles... after September 27, 2004. Inaccessible track means a method of establishing working limits on...

  18. Fungal Planet description sheets: 214–280

    NARCIS (Netherlands)

    Crous, P.W.; Shivas, R.G.; Quaedvlieg, W.; Bank, van der M.; Zhang, Y.; Summerell, B.A.; Guarro, J.; Wingfield, M.J.; Wood, A.R.; Alfenas, A.C.; Braun, U.; Cano-Lira, J.F.; García, D.; Marin-Felix, Y.; Alvarado, P.; Andrade, J.P.; Armengol, J.; Assefa, A.; Breeÿen, den A.; Camele, I.; Cheewangkoon, R.; De Souza, J.T.; Duong, T.A.; Esteve-Raventós, F.; Fournier, J.; Frisullo, S.; García-Jiménez, J.; Gardiennet, A.; Gené, J.; Hernández-Restrepo, M.; Hirooka, Y.; Hospenthal, D.R.; King, A.; Lechat, C.; Lombard, L.; Mang, S.M.; Marbach, P.A.S.; Marincowitz, S.; Marin-Felix, Y.; Montaño-Mata, N.J.; Moreno, G.; Perez, C.A.; Pérez Sierra, A.M.; Robertson, J.L.; Roux, J.; Rubio, E.; Schumacher, R.K.; Stchigel, A.M.; Sutton, D.A.; Tan, Y.P.; Thompson, E.H.; Linde, van der E.; Walker, A.K.; Walker, D.M.; Wickes, B.L.; Wong, P.T.W.; Groenewald, J.Z.

    2014-01-01

    Novel species of microfungi described in the present study include the following from South Africa: Cercosporella dolichandrae from Dolichandra unguiscati, Seiridium podocarpi from Podocarpus latifolius, Pseudocercospora parapseudarthriae from Pseudarthria hookeri, Neodevriesia coryneliae from Coryn

  19. BDML Metadata: 214 [SSBD[Archive

    Lifescience Database Archive (English)

    Full Text Available NC-SA 0.150 f5a7a976-0215-4b87-8dfd-5a952cc85fe2 0.105 x 0.105 x 0.5 (micrometer), 40 (second) http://ssbd.qbic.riken.jp/data.../source/Ce_KK_P002/RNAi_T20G5.1_040907_01/ http://ssbd.qbic.riken.jp/data.../bdml/Ce_KK_P002/RNAi_T20G5.1_040907_01.bdml0.15.xml http://ssbd.qbic.riken.jp/data/pdpml/Ce_KK_P00...2.pdpml0.05.xml http://ssbd.qbic.riken.jp/search/f5a7a976-0215-4b87-8dfd-5a952cc85fe2/ http://ssbd.qbic.riken.jp/omero/webclient/?show=dataset-157 ...

  20. GPCR Interaction: 214 [GRIPDB[Archive

    Lifescience Database Archive (English)

    Full Text Available s controlled by RTP4, which governs their membrane targeting and functional activity. 18836069 ELISA, Immunoblotting, Coimmunoprecipi...tation, Western blotting, SEAP assay NP_000905.3 ...

  1. 24 CFR 5.214 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... HUD PROGRAM REQUIREMENTS; WAIVERS Disclosure and Verification of Social Security Numbers and Employer... homeowner or cooperative member seeking homeownership assistance (including where the individual seeks to... responsibilities and obligations of the parties participating in a computer match. Consent form means any consent...

  2. 214 Using Appropriate Strategies to Improve Students ...

    African Journals Online (AJOL)

    User

    2011-07-21

    Jul 21, 2011 ... difficulties, meta-cognitive reading strategies, graphic organizers. Introduction .... reading materials and to learn the language of a discipline. Because ..... annual conference of the Nigerian English studies Association. (NESA) ...

  3. 47 CFR 15.214 - Cordless telephones.

    Science.gov (United States)

    2010-10-01

    ... is intended to be connected to the public switched telephone network shall also comply with the... protection against unintentional access to the public switched telephone network by the base unit and... network or ringing of the handset is preceded by the transmission of a code word. Access to the...

  4. 31 CFR 0.214 - Nondiscrimination.

    Science.gov (United States)

    2010-07-01

    ... official business on the basis of race, color, religion, national origin, sex, sexual orientation, age, or disability. Sexual harassment is a form of sex discrimination and is prohibited by this section. (b) An... does not create any enforceable legal rights in any person....

  5. Clinical performance of automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance assay in diagnosis of pulmonary tuberculosis in 214 cases%利福平耐药结核分枝杆菌实时荧光定量核酸扩增检测技术在214例诊断肺结核患者中的临床应用评价

    Institute of Scientific and Technical Information of China (English)

    何贵清; 李涛; 施伎蝉; 宁洪叶; 吴祥兵; 蔡明明; 吴正兴; 胡陈婵; 蒋贤高

    2016-01-01

    目的:评估利福平耐药结核分枝杆菌实时荧光定量核酸扩增检测技术(Xpert M TB/RIF)在温州地区诊断肺结核及利福平耐药的临床应用价值。方法纳入可疑肺结核、临床诊断肺结核和可疑耐药肺结核患者214例,采集痰标本同时送检抗酸染色涂片、液体培养和 Xpert M TB/RIF 检测。以临床最终诊断结果为金标准,比较三种方法检测结核分枝杆菌的敏感度、特异度。以液体药物敏感试验结果为金标准,Xpert M TB/RIF 检测利福平耐药的敏感度和特异度。率的比较采取卡方检验。结果以临床最终诊断结果作为金标准,Xpert M TB/RIF 检测结核分枝杆菌的敏感度高于抗酸染色涂片(69.5%比44.1%,χ2=23.31,P <0.01),而与液体培养比较敏感度差异无统计学意义(69.5%比62.1%,χ2=2.15,P>0.05);Xpert M TB/RIF 在痰涂片阳性与阴性的标本中检测结核分枝杆菌的敏感度分别为97.4%和47.5%,在痰涂片阳性与阴性的标本中检测结核分枝杆菌的特异度均为100.0%。以液体药物敏感试验结果为金标准,Xpert M TB/RIF 检测痰标本利福平耐药的敏感度和特异度分别为92.9%和98.8%。结论 Xpert M TB/RIF 能够快速、准确地检测结核分枝杆菌及其利福平耐药性,且敏感度较高,具有很好的应用价值。%Objective To evaluate the clinical application of the automated nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance (Xpert M TB/RIF) in diagnosis of pulmonary tuberculosis in Wenzhou .Methods A total of 214 patients with suspected pulmonary tuberculosis , clinical diagnosed tuberculosis or suspected drug‐resistant tuberculosis were enrolled in this study .The patients′ sputum specimens were collected for acid‐fast smear ,liquid culture and Xpert M TB/RIF assay .The sensitivity

  6. The Technological Enhancement of Normally Occurring Radioactive Materials in Red Mud due to the Production of Alumina

    Directory of Open Access Journals (Sweden)

    Maurice O. Miller

    2016-01-01

    Full Text Available This study investigates the level of technological enhancement of normally occurring radioactive materials (TENORM in the red mud waste due to the production of alumina in Jamaica. Technological enhancements factors (TEF were determined for the uranium, thorium, actinium series, their progenies, and the nonseries potassium-40 using gamma spectrometry. The study concluded that bauxite production technologically enhances the uranium progenies Th-234, Pb-214, Bi-214, and Pa-234 and the thorium-232 progenies Ac-228, Pb-212, and Bi-212 in red mud. The actinium series was technologically enhanced, but K-40 and the thorium daughter, Tl-208, were reduced. The spectrometric comparison of Tl-208 (at 510 keV was unexpected since its other photopeaks at 583 keV, 934 keV, and 968 keV were markedly different. An explanation for this anomaly is discussed. An explanation regarding the process of accumulation and fractionation of organically derived phosphate deposits and potassium-feldspar is offered to explain the spectrometric differences between the alumina product and its waste material, red mud.

  7. 49 CFR 571.214 - Standard No. 214; Side impact protection.

    Science.gov (United States)

    2010-10-01

    ... surface of the door in an inboard direction normal to a vertical plane along the vehicle's longitudinal... longitudinal centerline of the vehicle. The pretest vehicle attitude is equal to the fully loaded attitude ± 10... vehicle. The longitudinal centerline of the moving deformable barrier is perpendicular to the...

  8. Purification of radium-226 for the manufacturing of actinium-225 in a cyclotron for alpha-immunotherapy; Radium-Aufreinigung zur Herstellung von Actinium-225 am Zyklotron fuer die Alpha-Immuntherapie

    Energy Technology Data Exchange (ETDEWEB)

    Marx, Sebastian Markus

    2014-09-23

    The thesis describes the development of methods for the purification of Ra-226. The objective was to obtain the radionuclide in the quality that is needed to be used as starting material in the manufacturing process for Ac-225 via proton-irradiated Ra-226. The radionuclide has been gained efficiently out of huge excesses of impurities. The high purity of the obtained radium affords its use as staring material in a pharmaceutical manufacturing process.

  9. Production of actinium, thorium and radium isotopes from natural thorium irradiated with protons up to 141 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Ermolaev, S.V.; Zhuikov, B.L.; Kokhanyuk, V.M.; Matushko, V.L. [Russian Academy of Sciences, Moscow (Russian Federation). Inst. of Nuclear Research; Kalmykov, S.N. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Chemistry Dept.; Aliev, R.A. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Skobeltsyn Inst. of Nuclear Physics; Tananaev, I.G.; Myasoedov, B.F. [Russian Academy of Sciences, Moscow (Russian Federation). A.N. Frumkin Inst. of Physical Chemistry and Electrochemistry

    2012-07-01

    Cross sections of {sup 225}Ac, {sup 227}Ac, {sup 227}Th and {sup 228}Th in thorium-232 targets irradiated with protons in the energy range 21-141 MeV have been measured. Based on these data, production yields of {sup 225}Ac and {sup 223}Ra in thick thorium targets have been calculated. It is possible to produce in proton energy range 60-140 MeV about 96 GBq (2.6 Ci) {sup 225}Ac per 10-d irradiation with 100 {mu}A proton beam current and 10-d decay, and much higher amount of {sup 223}Ra. The impurities of {sup 227}Ac and {sup 224}Ra are important and need to be assessed for further medical applications. (orig.)

  10. Renal uptake of bismuth-213 and its contribution to kidney radiation dose following administration of actinium-225-labeled antibody

    Energy Technology Data Exchange (ETDEWEB)

    Schwartz, J; O' Donoghue, J A; Humm, J L [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); Jaggi, J S [Bristol-Myers Squibb, Plainsboro, NJ (United States); Ruan, S; Larson, S M [Nuclear Medicine Service Department of Radiology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); McDevitt, M; Scheinberg, D A, E-mail: schwarj1@mskcc.org [Molecular Pharmacology and Chemistry, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10065 (United States)

    2011-02-07

    Clinical therapeutic studies using {sup 225}Ac-labeled antibodies have begun. Of major concern is renal toxicity that may result from the three alpha-emitting progeny generated following the decay of {sup 225}Ac. The purpose of this study was to determine the amount of {sup 225}Ac and non-equilibrium progeny in the mouse kidney after the injection of {sup 225}Ac-huM195 antibody and examine the dosimetric consequences. Groups of mice were sacrificed at 24, 96 and 144 h after injection with {sup 225}Ac-huM195 antibody and kidneys excised. One kidney was used for gamma ray spectroscopic measurements by a high-purity germanium (HPGe) detector. The second kidney was used to generate frozen tissue sections which were examined by digital autoradiography (DAR). Two measurements were performed on each kidney specimen: (1) immediately post-resection and (2) after sufficient time for any non-equilibrium excess {sup 213}Bi to decay completely. Comparison of these measurements enabled estimation of the amount of excess {sup 213}Bi reaching the kidney ({gamma}-ray spectroscopy) and its sub-regional distribution (DAR). The average absorbed dose to whole kidney, determined by spectroscopy, was 0.77 (SD 0.21) Gy kBq{sup -1}, of which 0.46 (SD 0.16) Gy kBq{sup -1} (i.e. 60%) was due to non-equilibrium excess {sup 213}Bi. The relative contributions to renal cortex and medulla were determined by DAR. The estimated dose to the cortex from non-equilibrium excess {sup 213}Bi (0.31 (SD 0.11) Gy kBq{sup -1}) represented {approx}46% of the total. For the medulla the dose contribution from excess {sup 213}Bi (0.81 (SD 0.28) Gy kBq{sup -1}) was {approx}80% of the total. Based on these estimates, for human patients we project a kidney-absorbed dose of 0.28 Gy MBq{sup -1} following administration of {sup 225}Ac-huM195 with non-equilibrium excess {sup 213}Bi responsible for approximately 60% of the total. Methods to reduce renal accumulation of radioactive progeny appear to be necessary for the success of {sup 225}Ac radioimmunotherapy.

  11. 27 CFR 9.214 - Haw River Valley.

    Science.gov (United States)

    2010-04-01

    ... the unnamed road known locally as Baynes Road 2 miles to its intersection with North Carolina State Highway 119 at Baynes in Caswell County; then (16) Proceed generally south-southeast along North Carolina...

  12. Dicty_cDB: AFI214 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available --- ---SLLFKMSTKFMNVDLMTKVINAV*rftmidvh*dvphgheckvdhngkeccvrshrp pppevcslrcppkheckfddhgkkccvkihcdevcdldcgrgfeckirhdgskccvrs...rftmidvh*dvphgheckvdhngkeccvrshrp pppevcslrcppkheckfddhgkkccvkihcdevcdldcgrgfeckirhdgskccvrser phppqhekcnkrc

  13. Dicty_cDB: VFA214 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available KIMDIIIQFFLLQDYQSIDNILNQG ISINTQNQEGVTLLQLAILKQNIPYVKYLIFKGANLNLGDLTEXTPLHKAVSIGSMDLVQ LLVQNGANLNSQDEEGDSPSH...KIMDIIIQFFLLQDYQSIDNILNQG ISINTQNQEGVTLLQLAILKQNIPYVKYLIFKGANLNLGDLTEXTPLHKAVSIGS

  14. 25 CFR 214.13 - Diligence; annual expenditures; mining records.

    Science.gov (United States)

    2010-04-01

    ... of the drill holes, to justify the expenditure, the sinking of a shaft to the ore body, and the... ores by drilling within 1 year test holes aggregating 2,000 feet unless a sufficient ore body is discovered to justify the sinking of a shaft to the ore body and the erecting of a mill when such tract...

  15. Parenting and Career Development. ERIC Digest No. 214.

    Science.gov (United States)

    Kerka, Sandra

    Research indicates that parenting styles, family functioning, and parent-child interaction influence career development. The authoritative parenting style is associated with self-confidence, persistence, social competence, academic success, and psychosocial development; parents provide a warm family climate, set standards, and promote independence…

  16. 2-[1-(4-Bromophenyl-2-nitroethyl]hexanoic acid

    Directory of Open Access Journals (Sweden)

    Yanpeng Zhang

    2014-05-01

    Full Text Available In the crystal structure of the title compoud, C14H18BrNO4, molecules are linked by a strong O—H...O hydrogen bond and weaker C—H...O interactions. The benzene ring makes dihedral angles of 3.67 (3 and 72.63 (3° with the carboxylic acid group and the nitro group, respectively.

  17. 13 CFR 400.214 - Participations in guaranteed loans.

    Science.gov (United States)

    2010-01-01

    ...) Subject to paragraphs (b), (c) and (d) of this section, a Lender may distribute the risk of a portion of a... solely responsible for the administration of the loan; and (3) The Board's ability to assert any and all... Lenders; (2) Private investment funds and insurance companies that do not usually invest in...

  18. 48 CFR 52.214-31 - Facsimile Bids.

    Science.gov (United States)

    2010-10-01

    ... a bid that is transmitted to and received by the Government via electronic equipment that.... (d) Facsimile bids must contain the required signatures. (e) The Government reserves the right to..., receiving speed, communications protocol): (g) If the bidder chooses to transmit a facsimile bid, the...

  19. Dicty_cDB: CHM214 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nthase kinase 3 mRNA, complete cds. 46 1e-05 2 AJ758518 |AJ758518.1 Gerbera hybrid cv. 'Terra Regina' EST, c...lone G0000400007C09F1. 60 5e-05 1 AJ759858 |AJ759858.1 Gerbera hybrid cv. 'Terra Regina' EST, clone G0000400...024D05F1. 60 5e-05 1 AJ758862 |AJ758862.1 Gerbera hybrid cv. 'Terra Regina' EST,

  20. 38 CFR 21.214 - Furnishing supplies for special programs.

    Science.gov (United States)

    2010-07-01

    ... veteran's course, VA will furnish those books and supplies which the school requires all students in the... the veteran's farm by an individual instructor, VA will furnish to a student only those textbooks and... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Furnishing supplies for...

  1. Dicty_cDB: CHA214 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2D11 5' sequence, mRNA sequence. 92 6e-16 3 AW217896 |AW217896.1 EST296610 tomato flower buds, anthesis, Cornell...-15 2 AI778006 |AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17...root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence. 9

  2. 48 CFR 52.214-5 - Submission of Bids.

    Science.gov (United States)

    2010-10-01

    ... authorized by the solicitation. (e) Bids submitted by electronic commerce shall be considered only if the electronic commerce method was specifically stipulated or permitted by the solicitation. (End of provision) ... submitted by electronic means) (1) addressed to the office specified in the solicitation, and (2)...

  3. 28 CFR 2.214 - Probable cause hearing and determination.

    Science.gov (United States)

    2010-07-01

    ... adverse witnesses (i.e., witnesses who have given information upon which revocation may be based) at a... confrontation. Whenever a probable cause hearing is postponed to secure the appearance of adverse witnesses...

  4. 49 CFR 214.523 - Hi-rail vehicles.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance Machines and Hi-Rail... designated by the employer. (c) A new hi-rail vehicle shall be equipped with: (1) An automatic change-of... change-of-direction alarms, backup alarms, and 360-degree intermittent warning lights or beacons shall be...

  5. 40 CFR 60.214 - Test methods and procedures.

    Science.gov (United States)

    2010-07-01

    ... (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for the Phosphate.../hr). K=conversion factor, 1000 mg/g (7,000 gr/lb). (2) Method 13A or 13B shall be used to...

  6. Dicty_cDB: SSB214 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1.18 Translated Amino Acid sequence knisyl*flifiyifiyyiytpfftyllnyfiikkiikikyniif**FKKLKKKKTQKKK KNQTFVNYCK...yifiyyiytpfftyllnyfiikkiikikyniif**FKKLKKKKTQKKK KNQTFVNYCKKKKKQKQKEKTMDLNKEW--- Frame C: kiyhiysf*ylfiylfii

  7. 49 CFR 214.5 - Responsibility for compliance.

    Science.gov (United States)

    2010-10-01

    ..., or has caused death or injury, a penalty not to exceed $100,000 per violation may be assessed. See... requirement is subject to a civil penalty of at least $650 and not more than $25,000 per violation, except that penalties may be assessed against individuals only for willful violations, and where a...

  8. Soil and river sediments radionuclides monitoring at Aramar Experimental Center: an historical overview

    Energy Technology Data Exchange (ETDEWEB)

    Segre, Nadia; Fagundes, Rosane Correa, E-mail: nadia.segre@ctmsp.mar.mil.br [Centro Tecnologico da Marinha (CTM-SP/CEA/LARE), Ipero, SP (Brazil). Centro Experimental Aramar. Lab. Radioecologico; Moraes, Marco Antonio P.V. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    In order to evaluate possible effects to the environment resulting from the implementation of the Centro Tecnologico da Marinha - Centro Experimental Aramar (CTMSP-CEA) at Ipero in Sao Paulo state, Brazil, which came into operation in 1989, an Environmental Monitoring Program (PMA) was established in October, 1987. One of the aims of this program is to monitor the soil and river sediments radionuclides levels at CEA and beyond its boundary. The utilization of statistical tools to evaluate the results of radiometric environmental monitoring is a procedure required by National Nuclear Energy Commission (CNEN). The box plot is a simple statistical tool for displaying data. The central tendency and dispersion of the results as well as the observation of unusual results (outliers) in the dataset are easily visualized. Control chart is a graph that maps data and provides a picture of how a process is performing over time. A control chart always has a central line for the mean, an upper line for the upper control limit and a lower line for the lower control limit. Box plots and control charts were used to visualize the annual amount of natural uranium, lead-214, actinium-228 and lead-212 in soil and river sediment detected between 1987 and 2011, considering the measurements of all monitored places each year. This historical observation shows that, in average, the results obtained are below than the 1987-1988 levels (CEA's pre-operational) or below than the backgrounds radionuclides values. (author)

  9. Vocabulary of global warming, Vol. I: Contributors to the greenhouse effect. Terminology bulletin No. 214

    Energy Technology Data Exchange (ETDEWEB)

    Rivard, D.

    1992-01-01

    The vocabulary contains approximately 6,000 terms, with definitions for some 1,250 concepts. It has been prepared in accordance with current terminological methods: Equivalence of terms was confirmed by consulting recent and reliable sources in both English and French. It is intended as a flexible tool for responding quickly to terminological needs in the environmental sciences. It includes an example of usage of the entry and defines the concept.

  10. 76 FR 50113 - Airworthiness Directives; Airbus Model A320-214, -232, and -233 Airplanes

    Science.gov (United States)

    2011-08-12

    ... above, this AD requires a modification of the electrical installation of ELAC2 and THS Motor 1 power... case of emergency electrical configuration combined with a Green and Yellow hydraulic system loss... INFORMATION CONTACT: Sanjay Ralhan, Aerospace Engineer, International Branch, ANM-116, Transport...

  11. 76 FR 19714 - Airworthiness Directives; Airbus Model A320-214, -232, and -233 Airplanes

    Science.gov (United States)

    2011-04-08

    ... a modification of the electrical installation of ELAC2 and THS Motor 1 power supply, restoring the... case of emergency electrical configuration combined with a Green and Yellow hydraulic system loss... receipt. FOR FURTHER INFORMATION CONTACT: Sanjay Ralhan, Aerospace Engineer, International Branch,...

  12. 23 CFR 450.214 - Development and content of the long-range statewide transportation plan.

    Science.gov (United States)

    2010-04-01

    ... TRANSPORTATION PLANNING AND RESEARCH PLANNING ASSISTANCE AND STANDARDS Statewide Transportation Planning and... long-range statewide transportation plan should include capital, operations and management strategies... transportation system. The long-range statewide transportation plan may consider projects and strategies that...

  13. Clinical pharmacology study of cariprazine (MP-214) in patients with schizophrenia (12-week treatment)

    Science.gov (United States)

    Nakamura, Tadakatsu; Kubota, Tomoko; Iwakaji, Atsushi; Imada, Masayoshi; Kapás, Margit; Morio, Yasunori

    2016-01-01

    Purpose Cariprazine is a potent dopamine D3-preferring D3/D2 receptor partial agonist in development for the treatment of schizophrenia, bipolar mania, and depression. Pharmacokinetics of cariprazine and the two clinically relevant metabolites (desmethyl- and didesmethyl-cariprazine) was evaluated in a clinical pharmacology study. Methods This was a multicenter, randomized, open-label, parallel-group, fixed-dose (3, 6, or 9 mg/day) study of 28-week duration (≤4-week observation, 12-week open-label treatment, and 12-week follow-up). Once-daily cariprazine was administered to 38 adult patients with schizophrenia. The pharmacokinetics of cariprazine, metabolites, and total active moieties (sum of cariprazine and two metabolites) was evaluated; efficacy and safety were also assessed. Results Steady state was reached within 1–2 weeks for cariprazine and desmethyl-cariprazine, 4 weeks for didesmethyl-cariprazine, and 3 weeks for total active moieties. Cariprazine and desmethyl-cariprazine levels decreased >90% within 1 week after the last dose, didesmethyl-cariprazine decreased ~50% at 1 week, and total active moieties decreased ~90% within 4 weeks. Terminal half-lives of cariprazine, desmethyl-cariprazine, and didesmethyl-cariprazine ranged from 31.6 to 68.4, 29.7 to 37.5, and 314 to 446 hours, respectively. Effective half-life (calculated from time to steady state) of total active moieties was ~1 week. Incidence of treatment-emergent adverse events was 97.4%; 15.8% of patients discontinued due to adverse events. No abnormal laboratory values or major differences from baseline in extrapyramidal symptoms were observed. Conclusion Cariprazine and its active metabolites reached steady state within 4 weeks, and exposure was dose proportional over the range of 3–9 mg/day. Once-daily cariprazine was generally well tolerated in adult patients with schizophrenia. PMID:26834462

  14. Complications and survival after surgical treatment of 214 metastatic lesions of the humerus

    DEFF Research Database (Denmark)

    Wedin, Rikard; Hansen, Bjarne H; Laitinen, Minna;

    2012-01-01

    The humerus is the second most common long-bone site of metastatic bone disease. We report complications, risk factors for failure, and survival of a large series of patients operated on for skeletal metastases of the humerus....

  15. 42 CFR 421.214 - Advance payments to suppliers furnishing items or services under Part B.

    Science.gov (United States)

    2010-10-01

    ... maintain a financial system of data in accordance with the Statement of Federal Financial Accounting... 42 Public Health 3 2010-10-01 2010-10-01 false Advance payments to suppliers furnishing items or... Advance payments to suppliers furnishing items or services under Part B. (a) Scope and applicability. This...

  16. 9 CFR 381.214 - Movement of poultry or other article detained; removal of official marks.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Movement of poultry or other article... INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION POULTRY PRODUCTS INSPECTION...

  17. Suction drain tip culture in orthopaedic surgery: a prospective study of 214 clean operations

    OpenAIRE

    Sankar, B.; P Ray; Rai, J.

    2004-01-01

    We conducted a prospective cohort study in order to determine whether suction drain specimen cultures from orthopaedic surgery predicted an early wound infection. We included 218 consecutive clean orthopaedic operations requiring drains in one unit over a period of 1 year. The suction drain tip, drain fluid and wound discharge specimens were cultured, and the surgical wound was followed up for 3 months. There were six deep and two superficial wound infections. Wound infection was significantl...

  18. Workshop on Survey Methods in Education Research: Facilitator's Guide and Resources. REL 2017-214

    Science.gov (United States)

    Walston, Jill; Redford, Jeremy; Bhatt, Monica P.

    2017-01-01

    This Workshop on Survey Methods in Education Research tool consists of a facilitator guide and workshop handouts. The toolkit is intended for use by state or district education leaders and others who want to conduct training on developing and administering surveys. The facilitator guide provides materials related to various phases of the survey…

  19. 8 CFR 214.11 - Alien victims of severe forms of trafficking in persons.

    Science.gov (United States)

    2010-01-01

    ... United States Attorneys' Offices, the Civil Rights and Criminal Divisions, the Federal Bureau of... Department of Justice, Civil Rights Division, Trafficking in Persons and Worker Exploitation Task Force... providing an LEA endorsement, and the Department of Health and Human Service's Office of Refugee...

  20. Corrigendum to "Is the Lishan Fault active?" [Tectonophysics volume no: 661, pages range: 210-214

    Science.gov (United States)

    Kuo-Chen, Hao; Wu, Francis; Chang, Wu-Lung; Chang, Chih-Yu; Cheng, Ching-Yu; Hirata, Naoshi

    2017-01-01

    The authors regret to inform that there is a need to make a correction for Fig. 1(d) caption to the published article. Please note that the authors wish to make the following changes to the above paper:

  1. Tank 214-AW-105, grab samples, analytical results for the finalreport

    Energy Technology Data Exchange (ETDEWEB)

    Esch, R.A.

    1997-02-20

    This document is the final report for tank 241-AW-105 grab samples. Twenty grabs samples were collected from risers 10A and 15A on August 20 and 21, 1996, of which eight were designated for the K Basin sludge compatibility and mixing studies. This document presents the analytical results for the remaining twelve samples. Analyses were performed in accordance with the Compatibility Grab Sampling and Analysis Plan (TSAP) and the Data Quality Objectives for Tank Farms Waste Compatibility Program (DO). The results for the previous sampling of this tank were reported in WHC-SD-WM-DP-149, Rev. 0, 60-Day Waste Compatibility Safety Issue and Final Results for Tank 241-A W-105, Grab Samples 5A W-95-1, 5A W-95-2 and 5A W-95-3. Three supernate samples exceeded the TOC notification limit (30,000 microg C/g dry weight). Appropriate notifications were made. No immediate notifications were required for any other analyte. The TSAP requested analyses for polychlorinated biphenyls (PCB) for all liquids and centrifuged solid subsamples. The PCB analysis of the liquid samples has been delayed and will be presented in a revision to this document.

  2. Tank 241-AX-103, cores 212 and 214 analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    Steen, F.H.

    1998-02-05

    This document is the analytical laboratory report for tank 241-AX-103 push mode core segments collected between July 30, 1997 and August 11, 1997. The segments were subsampled and analyzed in accordance with the Tank 241-AX-103 Push Mode Core Sampling and Analysis Plan (TSAP) (Comer, 1997), the Safety Screening Data Quality Objective (DQO) (Dukelow, et al., 1995) and the Data Quality Objective to Support Resolution of the Organic Complexant Safety Issue (Organic DQO) (Turner, et al., 1995). The analytical results are included in the data summary table (Table 1). None of the samples submitted for Differential Scanning Calorimetry (DSC), Total Alpha Activity (AT), plutonium 239 (Pu239), and Total Organic Carbon (TOC) exceeded notification limits as stated in the TSAP (Conner, 1997). The statistical results of the 95% confidence interval on the mean calculations are provided by the Tank Waste Remediation Systems Technical Basis Group in accordance with the Memorandum of Understanding (Schreiber, 1997) and not considered in this report.

  3. 23 CFR 971.214 - Federal lands congestion management system (CMS).

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Federal lands congestion management system (CMS). 971... Federal lands congestion management system (CMS). (a) For purposes of this section, congestion means the level at which transportation system performance is no longer acceptable due to traffic...

  4. 78 FR 22025 - Eighteenth Meeting: RTCA Special Committee 214, Standards for Air Traffic Data Communication...

    Science.gov (United States)

    2013-04-12

    ... & Change 1 to DO-281B (Dongsong Zeng) Approve TOR Chagens to add to the SC deliverables Change 1 to DO-224C... Communications Including Compatibility with Digital Voice Techniques'' Approve TOR Changes to add to the...

  5. 8 CFR 214.3 - Approval of schools for enrollment of F and M nonimmigrants.

    Science.gov (United States)

    2010-01-01

    ... crime; and (E) Any other notification request not covered by paragraph (g)(1) of this section made by... activities may include, but are not limited to, research projects and orientation sessions. The DSO may...

  6. 2-[1-(4-Bromophenyl-3-hydroxy-3-(4-methoxyphenylpropyl]cyclohexanol

    Directory of Open Access Journals (Sweden)

    Ísmail Çelik

    2013-07-01

    Full Text Available In the title compound, C22H27BrO3, the cyclohexane ring adopts a chair conformation. The dihedral angle between the benzene rings is 41.9 (4°. In the crystal, molecules are linked by O—H...O and C—H...O hydrogen bonds, forming a three-dimensional network. In addition, π–π stacking interactions [centroid–centroid distance = 3.953 (6 Å] between the benzene rings of the methoxybenzene groups occur.

  7. 40 CFR 2.214 - Defense of Freedom of Information Act suits; participation by affected business.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Defense of Freedom of Information Act... Freedom of Information Act suits; participation by affected business. (a) In making final confidentiality... Information Act for disclosure of that information, EPA will: (1) Notify each affected business of the...

  8. SU-E-T-214: Predicting Plan Quality from Patient Geometry: Feature Selection and Inference Modeling.

    Science.gov (United States)

    Ruan, D; Shao, W; DeMarco, J; Kupelian, P; Low, D

    2012-06-01

    To investigate and develop methods to infer treatment plan quality from the geometric features of PTV/OAR structures; to discover and identify features of high prognostic values. This study explores the prognostic utility of geometric features of two categories: (1) absolute geometry, characterizing the volumes of single structures (PTV, OARs); and (2) relative geometry, based on the minimal 3D distance and/or overlapping volume between pairs of structures. Using prostate as a pilot site, we developed inference models to 'predict' SBRT plan quality of DVH end points. We developed and assessed (1) a full linear regression model based on both absolute and relative geometric features, (2) a sparsity-penalized linear regression model, (3) a linear regression model based on absolute geometry features only; (4) a learning-based nonparametric model. Cross-validation was used for both selecting the parameter values as well as quantifying the inference performance. The best inference method for each of the DVH end points was identified to reveal the structural and prognostic differences among them. For linear regression, using sparsity-regularization discovered geometric features that were mostly absolute, demonstrating their dominant linear prognostic utility. However, introducing relative geometric features improved the plan quality prediction by 15% for all DVH end points. In contrast, nonparametric models had a heavier dependence on relative geometry features. While linear regression based on both features sets predicted OAR DVH points slightly better, the nonparametric method excelled in predicting PTV coverage and conformality. The inference result from this study provides an 'expectation' for the plan quality before the planning is to be performed, providing reference goals for the planner and a baseline for detecting abnormality. The use of relative geometry complements the absolute geometry with information on spatial configuration of the PTV/OAR structures of individual patients, and the variation in achievable conformality as a consequence. © 2012 American Association of Physicists in Medicine.

  9. 48 CFR 214.407-3 - Other mistakes disclosed before award.

    Science.gov (United States)

    2010-10-01

    .... (v) National Geospatial-Intelligence Agency: General Counsel, NGA. (vi) Defense Threat Reduction... Information Systems Agency: General Counsel, DISA. (iii) Defense Intelligence Agency: Principal Assistant...

  10. Health-hazard evaluation report HETA 84-214-1633, Sheldahl, Inc. , Northfield, Minnesota

    Energy Technology Data Exchange (ETDEWEB)

    Almaguer, D.; Seligman, P.

    1985-11-01

    In response to a request from Amalgamated Clothing and Textiles Workers Union, an evaluation was made of possible hazards at Sheldahl, Inc., Northfield, Minnesota. The facility produced flexible printed circuitry. At the facility, adhesives were mixed including solid resins such as polyester, epoxy or nylon; solvents such as methylene chloride, methyl ethyl ketone, acetone, and smaller quantities of 1,1,2-trichloroethane, methanol, and others; and additives such as powders, dyes, and isocyanate curing agents. Samples collected in lamination departments indicated workers were potentially exposed to methylene chloride at levels ranging from 26 to 132 parts per million (ppm). Methylene chloride concentrations for short-term exposures were calculated to range up to 1752 ppm. A potential health hazard from exposure to methylene chloride exists. A less toxic material should be substituted for methylene chloride. Exhaust ventilation systems and personal protective equipment should be upgraded. Solvent-soaked rags should be placed in sealable containers. Employee education should be undertaken, and medical monitoring should be conducted on a regular basis.

  11. 47 CFR 63.21 - Conditions applicable to all international Section 214 authorizations.

    Science.gov (United States)

    2010-10-01

    ... continuing accuracy of the certifications made in its application. Whenever the substance of any such... of this chapter. Except as specified in § 20.15(d) of this chapter with respect to commercial mobile... carrier in the course of its normal business dealings, unless the competing U.S. carrier provides...

  12. 48 CFR 52.214-26 - Audit and Records-Sealed Bidding.

    Science.gov (United States)

    2010-10-01

    ..., documents, accounting procedures and practices, and other data, regardless of type and regardless of whether such items are in written form, in the form of computer data, or in any other form. (b) Certified cost... representative of the Contracting Officer, in order to evaluate the accuracy, completeness, and currency of the...

  13. Air Force Manual 33-214 Volume 2. Communications and Information. Emission Security Countermeasures Reviews

    Science.gov (United States)

    2007-11-02

    active devices in them (e.g., computer chips , junctions, registers, etc.); mechanical switches or mechanical relays do all the switching. Active control...to be reflected. The best solution is to use a closed room, keeping the doors closed and covering the windows with drapes . 3.4.7. Cordless...windows with drapes . 3.4.10. Infrared Devices. These countermeasures are required. Infrared devices not covered by any subparagraph of paragraph 3.4

  14. 47 CFR 63.09 - Definitions applicable to international Section 214 authorizations.

    Science.gov (United States)

    2010-10-01

    ... telecommunications services offered to the public in that country within the meaning of the International... carriers are parties to, or the beneficiaries of, a contractual relation (e.g., a joint venture or market alliance) affecting the provision or marketing of international basic telecommunications services in...

  15. 49 CFR 214.105 - Fall protection systems standards and practices.

    Science.gov (United States)

    2010-10-01

    ... limits of the equipment, proper hook-up, anchoring and tie-off techniques, methods of use, and proper methods of equipment inspection and storage. (6) The railroad or railroad contractor shall provide...

  16. Brief 72 Nuclear Engineering Enrollments and Degrees Survey, 2013 Data (2-14)

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2014-02-15

    The survey includes degrees granted between September 1, 2012 and August 31, 2013. Enrollment information refers to the fall term 2013. The enrollments and degrees data include students majoring in nuclear engineering or in an option program equivalent to a major. Thirty-two academic programs reported having nuclear engineering programs during 2013, and data was received from all thirty-two programs. The data for two nuclear engineering programs include enrollments and degrees in health physics options that are also reported in the health physics enrollments and degrees data.

  17. 8 CFR 214.1 - Requirements for admission, extension, and maintenance of status.

    Science.gov (United States)

    2010-01-01

    ...)(15)(U)(ii) U-2, U-3, U-4, U-5 101(a)(15)(V) V-1, V-2, or V-3 NAFTA, Principal TN. NAFTA, Dependent TD... (or on or before July 26, 2005, in the case of a citizen of Canada or Mexico, who, before September 23...

  18. 28 CFR 21.4 - Fees and allowances of fact witnesses.

    Science.gov (United States)

    2010-07-01

    ... transportation expenses. A witness shall be entitled to transportation expenses based on the means of transportation reasonably utilized (based on the nature, duration, location and distance of travel) and the... single items costing more than $25. (4) First-class travel by witnesses requires the same...

  19. 8 CFR 214.2 - Special requirements for admission, extension, and maintenance of status.

    Science.gov (United States)

    2010-01-01

    ... forth in Appendix 1603.A.1 to Annex 1603 of the NAFTA—(A) Research and design. Technical scientific and statistical researchers conducting independent research or research for an enterprise located in the territory... transactions for an enterprise located in the territory of another Party. (C) Marketing. (1) Market...

  20. 77 FR 214 - Revisions to the California State Implementation Plan, San Joaquin Valley Unified Air Pollution...

    Science.gov (United States)

    2012-01-04

    ... Air Pollution Control District (SJVUAPCD) AGENCY: Environmental Protection Agency (EPA). ACTION: Final rule. SUMMARY: EPA is finalizing approval of revisions to the San Joaquin Valley Air Pollution Control... and air districts for evaluating air pollution control economics. 3. Farms can change owners...

  1. An aerial radiological survey of the Nevada Test Site

    Energy Technology Data Exchange (ETDEWEB)

    Hendricks, T J; Riedhauser, S R

    1999-12-01

    A team from the Remote Sensing Laboratory conducted an aerial radiological survey of the US Department of Energy's Nevada Test Site including three neighboring areas during August and September 1994. The survey team measured the terrestrial gamma radiation at the Nevada Test Site to determine the levels of natural and man-made radiation. This survey included the areas covered by previous surveys conducted from 1962 through 1993. The results of the aerial survey showed a terrestrial background exposure rate that varied from less than 6 microroentgens per hour (mR/h) to 50 mR/h plus a cosmic-ray contribution that varied from 4.5 mR/h at an elevation of 900 meters (3,000 feet) to 8.5 mR/h at 2,400 meters (8,000 feet). In addition to the principal gamma-emitting, naturally occurring isotopes (potassium-40, thallium-208, bismuth-214, and actinium-228), the man-made radioactive isotopes found in this survey were cobalt-60, cesium-137, europium-152, protactinium-234m an indicator of depleted uranium, and americium-241, which are due to human actions in the survey area. Individual, site-wide plots of gross terrestrial exposure rate, man-made exposure rate, and americium-241 activity (approximating the distribution of all transuranic material) are presented. In addition, expanded plots of individual areas exhibiting these man-made contaminations are given. A comparison is made between the data from this survey and previous aerial radiological surveys of the Nevada Test Site. Some previous ground-based measurements are discussed and related to the aerial data. In regions away from man-made activity, the exposure rates inferred from the gamma-ray measurements collected during this survey agreed very well with the exposure rates inferred from previous aerial surveys.

  2. Orthorectification and Mosaicking of Color Aerial Photography for the Main Eight Hawaiian Islands: Oahu (213-214w-0516)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Habitat maps of the main Hawaiian Islands were created by visual interpretation of aerial photos and hyperspectral imagery using the Habitat Digitizer extension....

  3. 49 CFR 214.527 - On-track roadway maintenance machines; inspection for compliance and schedule for repairs.

    Science.gov (United States)

    2010-10-01

    ... operator's seat shall be replaced or repaired within 24 hours or by the start of the machine's next tour of duty, whichever is later. The machine may be operated for the remainder of the operator's tour of duty... roadway maintenance machines; inspection for compliance and schedule for repairs. (a) The operator of...

  4. 77 FR 29750 - Fifteenth Meeting: RTCA Special Committee 214, Joint With EUROCAE WG-78, Standards for Air...

    Science.gov (United States)

    2012-05-18

    ... will be held at Maastricht UAC, Eurocontrol Maastricht UAC, The Netherlands. Contact Christopher.Adams..., or Web site at http://www.rtca.org . Please confirm your attendance to Christopher.Adams@eurocontrol... Contributions Approval of Sub Approval of Sub-Group Meeting Objectives Day 2 (Tuesday) 900-1700:...

  5. 8 CFR 214.6 - Citizens of Canada or Mexico seeking temporary entry under NAFTA to engage in business activities...

    Science.gov (United States)

    2010-01-01

    ... —Dentist—D.D.S., D.M.D., Doctor en Odontologia or Doctor en Cirugia Dental or state/provincial license... Degree; or state/provincial license. —Physician (teaching or research only)—M.D. Doctor en Medicina;...

  6. Orthorectification and Mosaicking of Color Aerial Photography for the Main Eight Hawaiian Islands: Oahu (213-214e-0516)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Habitat maps of the main Hawaiian Islands were created by visual interpretation of aerial photos and hyperspectral imagery using the Habitat Digitizer extension....

  7. 78 FR 47480 - Nineteenth Meeting: RTCA Special Committee 214/EUROCAE WG-78: Standards for Air Traffic Data...

    Science.gov (United States)

    2013-08-05

    ... for Air Traffic Data Communication Services AGENCY: Federal Aviation Administration (FAA), U.S...: Standards for Air Traffic Data Communication Services meeting. SUMMARY: The FAA is issuing this notice to...-78: Standards for Air Traffic Data Communication Services. DATES: The meeting will be held August 26...

  8. 78 FR 76888 - Twentieth Meeting: RTCA Special Committee 214/EUROCAE WG-78: Standards for Air Traffic Data...

    Science.gov (United States)

    2013-12-19

    ... Air Traffic Data Communication Services AGENCY: Federal Aviation Administration (FAA), U.S. Department... for Air Traffic Data Communication Services meeting. SUMMARY: The FAA is issuing this notice to advise...: Standards for Air Traffic Data Communication Services. DATES: The meeting will be held January 13-17 from 9...

  9. 49 CFR 214.509 - Required visual illumination and reflective devices for new on-track roadway maintenance machines.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Required visual illumination and reflective... Required visual illumination and reflective devices for new on-track roadway maintenance machines. Each new on-track roadway maintenance machine shall be equipped with the following visual illumination...

  10. Precision and within- and between-day variation of bioimpedance parameters in children aged 2-14 years

    DEFF Research Database (Denmark)

    Andersen, Trine B; Jødal, Lars; Arveschoug, Anne;

    2011-01-01

    BACKGROUND & AIMS: Bioimpedance spectroscopy (BIS) offers the possibility to perform rapid estimates of fluid distribution and body composition. Few studies, however, have addressed the precision and biological variation in a pediatric population. Our objectives were to evaluate precision.......4-14.9 years) had one series measured on day one (precision population). Forty-four children had a second series on day one (within-day sub-population). Thirty-two children had a series measured on the next day (between-day sub-population). Each measurement series consisted of three repeated measurements...

  11. Observation of Hydrological Processes Using Remote Sensing. Chapter 2.14; Volume 2: The Science of Hydrology

    Science.gov (United States)

    Wilder, Peter (Editor); Su, Z.; Robeling, R. A.; Schulz, J.; Holleman, I.; Levizzani, V.; Timmermans, W. J.; Rott, H.; Mognard-Campbell, N.; de Jeu, R.; hide

    2011-01-01

    Improving water management can make a significant contribution to achieving most of the Millennium Development Goals established by the UN General Assembly in 2000, especially those related to poverty, hunger, and major diseases. The World Summit on Sustainable Development (WSSD) in 2002 recognized this need. Water and sanitation in particular received great attention from the Summit. The Johannesburg Plan of Implementation recommended to improve water resources management and scientific understanding of the water cycle through joint cooperation and research. For this purpose, it is recommended to promote knowledge sharing, provide capacity building, and facilitate the transfer of technology including remote-sensing (RS) and satellite technologies, especially to developing countries and countries with economies in transition, and to support these countries in their efforts to monitor and assess the quantity and quality of water resources, for example, by establishing and/or further developing national monitoring networks and water resources databases and by developing relevant national indicators. The Johannesburg Plan also adopted integrated water resources management as the overarching concept in addressing and solving water-related issues. As a result of the commitments made in the Johannesburg Plan of Implementation, several global and regional initiatives have emerged. Current international initiatives such as the Global Monitoring for Environment and Security (GMES) program of the European Commission and the European Space Agency (ESA), and the Global Earth Observation System of Systems (GEOSS) 10-Year Implementation Plan, have all identified Earth observation (EO) of the water cycle as the key in helping to solve the world s water problems. The availability of spatial information on water quantity and quality will also enable closure of the water budget at river basin and continental scales to the point where effective water management is essential (e.g., as requested by the European Union s Water Framework Directive (WFD), as well as national policies). Geo-information science and EO are vital in achieving a better understanding of the water cycle and better monitoring, analysis, prediction, and management of the world s water resources. The major components of the water cycle of the Earth system and their possible observations are presented. Such observations are essential to understand the global water cycle and its variability, both spatially and temporally, and can only be achieved consistently by means of EOs. Additionally, such observations are essential to advance our understanding of coupling between the terrestrial, atmospheric, and oceanic branches of the water cycle, and how this coupling may influence climate variability and predictability. Water resources management directly interferes with the natural water cycle in the forms of building dams, reservoirs, water transfer systems, and irrigation systems that divert and redistribute part of the water storages and fluxes on land. The water cycle is mainly driven and coupled to the energy cycle in terms of phase changes of water (changes among liquid, water vapor, and solid phases) and transport of water by winds in addition to gravity and diffusion processes. The water-cycle components can be observed with in situ sensors as well as airborne and satellite sensors in terms of radiative quantities. Processing and conversion of these radiative signals are necessary to retrieve the water-cycle components.

  12. Oahu Photomosaic 2000 (213-214e-0516) - Orthorectification and Mosaicing of Color Aerial Photography Main Eight Hawaiian Islands

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Habitat maps of the main Hawaiian Islands were created by visual interpretation of aerial photos and hyperspectral imagery using the Habitat Digitizer extension....

  13. 49 CFR 214.511 - Required audible warning devices for new on-track roadway maintenance machines.

    Science.gov (United States)

    2010-10-01

    ... Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE...) An automatic change-of-direction alarm which provides an audible signal that is at least three seconds long and is distinguishable from the surrounding noise. Change of direction alarms may be...

  14. 48 CFR 52.214-27 - Price Reduction for Defective Certified Cost or Pricing Data-Modifications-Sealed Bidding.

    Science.gov (United States)

    2010-10-01

    ... were not complete, accurate, and current as certified in its Certificate of Current Cost or Pricing... Current Cost or Pricing Data, or (3) any of these parties furnished data of any description that were not... accurate, complete, and current certified cost or pricing data had been submitted; (ii) The...

  15. Observation of Hydrological Processes Using Remote Sensing. Chapter 2.14; Volume 2: The Science of Hydrology

    Science.gov (United States)

    Wilder, Peter (Editor); Su, Z.; Robeling, R. A.; Schulz, J.; Holleman, I.; Levizzani, V.; Timmermans, W. J.; Rott, H.; Mognard-Campbell, N.; de Jeu, R.; Wagner, W.; Rodell, M.; Salama, M. S.; Parodi, G. N.; Wang, L.

    2011-01-01

    Improving water management can make a significant contribution to achieving most of the Millennium Development Goals established by the UN General Assembly in 2000, especially those related to poverty, hunger, and major diseases. The World Summit on Sustainable Development (WSSD) in 2002 recognized this need. Water and sanitation in particular received great attention from the Summit. The Johannesburg Plan of Implementation recommended to improve water resources management and scientific understanding of the water cycle through joint cooperation and research. For this purpose, it is recommended to promote knowledge sharing, provide capacity building, and facilitate the transfer of technology including remote-sensing (RS) and satellite technologies, especially to developing countries and countries with economies in transition, and to support these countries in their efforts to monitor and assess the quantity and quality of water resources, for example, by establishing and/or further developing national monitoring networks and water resources databases and by developing relevant national indicators. The Johannesburg Plan also adopted integrated water resources management as the overarching concept in addressing and solving water-related issues. As a result of the commitments made in the Johannesburg Plan of Implementation, several global and regional initiatives have emerged. Current international initiatives such as the Global Monitoring for Environment and Security (GMES) program of the European Commission and the European Space Agency (ESA), and the Global Earth Observation System of Systems (GEOSS) 10-Year Implementation Plan, have all identified Earth observation (EO) of the water cycle as the key in helping to solve the world s water problems. The availability of spatial information on water quantity and quality will also enable closure of the water budget at river basin and continental scales to the point where effective water management is essential (e.g., as requested by the European Union s Water Framework Directive (WFD), as well as national policies). Geo-information science and EO are vital in achieving a better understanding of the water cycle and better monitoring, analysis, prediction, and management of the world s water resources. The major components of the water cycle of the Earth system and their possible observations are presented. Such observations are essential to understand the global water cycle and its variability, both spatially and temporally, and can only be achieved consistently by means of EOs. Additionally, such observations are essential to advance our understanding of coupling between the terrestrial, atmospheric, and oceanic branches of the water cycle, and how this coupling may influence climate variability and predictability. Water resources management directly interferes with the natural water cycle in the forms of building dams, reservoirs, water transfer systems, and irrigation systems that divert and redistribute part of the water storages and fluxes on land. The water cycle is mainly driven and coupled to the energy cycle in terms of phase changes of water (changes among liquid, water vapor, and solid phases) and transport of water by winds in addition to gravity and diffusion processes. The water-cycle components can be observed with in situ sensors as well as airborne and satellite sensors in terms of radiative quantities. Processing and conversion of these radiative signals are necessary to retrieve the water-cycle components.

  16. 29 CFR 778.214 - Benefit plans; including profit-sharing plans or trusts providing similar benefits.

    Science.gov (United States)

    2010-07-01

    ... fide plan for providing old age, retirement, life, accident, or health insurance or similar benefits... such employees. Accordingly, reference should be made to § 5.32 of this title as well as to § 778.215... satisfaction of his obligation to provide the specified benefits are also excludable from the regular rate...

  17. 9 CFR 205.214 - Litigation as to whether a system is operating in compliance with the Section.

    Science.gov (United States)

    2010-01-01

    ... as to whether a system is operating in compliance with the Section. (a) The requirements for a system... certification, is operating in compliance, thus whether it is a “central filing system” as defined, could be... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Litigation as to whether a system...

  18. 2-[1-(4-Ethoxyphenyl-2-oxo-4-styrylazetidin-3-yl]isoindoline-1,3-dione

    Directory of Open Access Journals (Sweden)

    Orhan Büyükgüngör

    2008-05-01

    Full Text Available The title compound, C27H22N2O4, contains a nearly planar four-membered β-lactam ring, which makes dihedral angles of 74.64 (12, 1.70 (11 and 73.67 (12° with the nine-membered ring system, the benzene ring and the phenyl ring, respectively. The crystal structure is stabilized by C—H...O and C—H...π interactions and a π–π interaction [centroid–centroid distance = 3.4505 (19 Å] is also present.

  19. 42 CFR 57.214 - Repayment of loans made after November 17, 1971, for failure to complete a program of study.

    Science.gov (United States)

    2010-10-01

    ..., adjusted annually for changes in the Consumer Price Index, and adjusted by the Secretary for use in this... undertakes to repay educational loans under section 722(k) of the Act, he or she will use the...

  20. Éloi Laurent et Jacques Le Cacheux, 2012, Économie de l’Environnement et Économie Écologique, Paris, Armand Colin, 214 pages.

    OpenAIRE

    2014-01-01

    Le défi relevé par Éloi Laurent et Jacques Le Cacheux est de résumer l’ensemble de l’analyse économique des questions environnementales, en regroupant à la fois la diversité des courants de pensée et celle des problématiques environnementales, dans un ouvrage accessible à un large public. Les auteurs sont tous deux économistes au département des Études de l’Observatoire Français des Conjonctures Economiques (OFCE). Éloi Laurent est spécialisé dans les questions relatives au développement dura...

  1. 4-Pregnen-21-ol-3,20-dione-21-(4-bromobenzenesulfonate) (NSC 88915) and related novel steroid derivatives as tyrosyl-DNA phosphodiesterase (Tdp1) inhibitors.

    Science.gov (United States)

    Dexheimer, Thomas S; Gediya, Lalji K; Stephen, Andrew G; Weidlich, Iwona; Antony, Smitha; Marchand, Christophe; Interthal, Heidrun; Nicklaus, Marc; Fisher, Robert J; Njar, Vincent C; Pommier, Yves

    2009-11-26

    Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is an enzyme that catalyzes the hydrolysis of 3'-phosphotyrosyl bonds. Such linkages form in vivo when topoisomerase I (Top1) processes DNA. For this reason, Tdp1 has been implicated in the repair of irreversible Top1-DNA covalent complexes. Tdp1 inhibitors have been regarded as potential therapeutics in combination with Top1 inhibitors, such as the camptothecin derivatives, topotecan, and irinotecan, which are used to treat human cancers. Using a novel high-throughput screening assay, we have identified the C21-substituted progesterone derivative, NSC 88915 (1), as a potential Tdp1 inhibitor. Secondary screening and cross-reactivity studies with related DNA processing enzymes confirmed that compound 1 possesses specific Tdp1 inhibitory activity. Deconstruction of compound 1 into discrete functional groups reveals that both components are required for inhibition of Tdp1 activity. Moreover, the synthesis of analogues of compound 1 has provided insight into the structural requirements for the inhibition of Tdp1. Surface plasmon resonance shows that compound 1 binds to Tdp1, whereas an inactive analogue fails to interact with the enzyme. On the basis of molecular docking and mechanistic studies, we propose that these compounds are competitive inhibitors, which mimics the oligonucleotide-peptide Tdp1 substrate. These steroid derivatives represent a novel chemotype and provide a new scaffold for developing small molecule inhibitors of Tdp1.

  2. 4-Pregnen-21-ol-3,20-dione-21-(4-bromobenzenesufonate) (NSC 88915) and Related Novel Steroid Derivatives as Tyrosyl-DNA Phosphodiesterase (Tdp1) Inhibitors

    Science.gov (United States)

    Dexheimer, Thomas S.; Gediya, Lalji K.; Stephen, Andrew G.; Weidlich, Iwona; Antony, Smitha; Marchand, Christophe; Interthal, Heidrun; Nicklaus, Marc; Fisher, Robert J.; Njar, Vincent C.; Pommier, Yves

    2009-01-01

    Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is an enzyme that catalyzes the hydrolysis of 3'-phosphotyrosyl bonds. Such linkages form in vivo when topoisomerase I (Top1) processes DNA. For this reason, Tdp1 has been implicated in the repair of irreversible Top1-DNA covalent complexes. Tdp1 inhibitors have been regarded as potential therapeutics in combination with Top1 inhibitors, such as the camptothecin derivatives, topotecan and irinotecan, which are used to treat human cancers. Using a novel high-throughput screening assay, we have identified the C21-substituted progesterone derivative, NSC 88915 (1), as a potential Tdp1 inhibitor. Secondary screening and cross-reactivity studies with related DNA processing enzymes confirmed that compound 1 possesses specific Tdp1 inhibitory activity. Deconstruction of compound 1 into discrete functional groups reveals that both components are required for inhibition of Tdp1 activity. Moreover, the synthesis of analogues of compound 1 has provided insight into the structural requirements for the inhibition of Tdp1. Surface plasmon resonance shows that compound 1 binds to Tdp1, whereas an inactive analogue fails to interact with the enzyme. Based on molecular docking and mechanistic studies, we propose that these compounds are competitive inhibitors, which mimics the oligonucleotide-peptide Tdp1 substrate. These steroid derivatives represent a novel chemotype and provide a new scaffold for developing small molecule inhibitors of Tdp1. PMID:19883083

  3. SU-E-J-214: MR Protocol Development to Visualize Sirius MRI Markers in Prostate Brachytherapy Patients for MR-Based Post-Implant Dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Lim, T; Wang, J; Frank, S; Stafford, R; Bruno, T; Bathala, T; Mahmood, U; Pugh, T; Ibbott, G; Kudchadker, R [UT MD Anderson Cancer Center, Houston, TX (United States)

    2015-06-15

    Purpose: The current CT-based post-implant dosimetry allows precise seed localization but limited anatomical delineation. Switching to MR-based post-implant dosimetry is confounded by imprecise seed localization. One approach is to place positive-contrast markers (Sirius) adjacent to the negative-contrast seeds. This patient study aims to assess the utility of a 3D fast spoiled gradient-recalled echo (FSPGR) sequence to visualize Sirius markers for post-implant dosimetry. Methods: MRI images were acquired in prostate implant patients (n=10) on Day 0 (day-of-implant) and Day 30. The post-implant MR protocol consisted of 3D T2-weighted fast-spin-echo (FSE), T2-weighted 2D-FSE (axial) and T1-weighted 2D-FSE (axial/sagittal/coronal). We incorporated a 3D-FSPGR sequence into the post-implant MR protocol to visualize the Sirius markers. Patients were scanned with different number-of-excitations (6, 8, 10), field-of-view (10cm, 14cm, 18cm), slice thickness (1mm, 0.8mm), flip angle (14 degrees, 20 degrees), bandwidth (122.070 Hz/pixel, 325.508 Hz/pixel, 390.625 Hz/pixel), phase encoding steps (160, 192, 224, 256), frequency-encoding direction (right/left, anterior/posterior), echo-time type (minimum-full, out-of-phase), field strength (1.5T, 3T), contrast (with, without), scanner vendor (Siemens, GE), coil (endorectal-coil only, endorectal-and-torso-coil, torsocoil only), endorectal-coil filling (30cc, 50cc) and endorectal-coil filling type (air, perfluorocarbon [PFC]). For post-implant dosimetric evaluation with greater anatomical detail, 3D-FSE images were fused with 3D-FSPGR images. For comparison with CT-based post-implant dosimetry, CT images were fused with 3D-FSPGR images. Results: The 3D-FSPGR sequence facilitated visualization of markers in patients. Marker visualization helped distinguish signal voids as seeds versus needle tracks for more definitive MR-based post-implant dosimetry. On the CT-MR fused images, the distance between the seed on CT to MR images was 3.2±1.6mm in patients with no endorectal coil, 2.3±0.8mm in patients with 30cc-PFC-filled endorectal-coil and 5.0±1.8mm in patients with 50cc-PFC-filled endorectal-coil. Conclusion: An MR protocol to visualize positive-contrast Sirius markers to assist in the identification of negative-contrast seeds was demonstrated. S Frank is a co-founder of C4 Imaging LLC, the manufacturer of the MRI markers.

  4. A homozygous nonsense mutation (c.214C->A) in the biliverdin reductase alpha gene (BLVRA) results in accumulation of biliverdin during episodes of cholestasis

    DEFF Research Database (Denmark)

    Nytofte, Nikolaj S; Serrano, Maria A; Monte, Maria J;

    2011-01-01

    Green jaundice is a rare finding usually associated with end-stage liver disease. OBJECTIVE The authors investigated two unrelated Inuit women from different geographical areas in Greenland who had episodes of green jaundice associated with biliary obstruction....

  5. Effect of various types of intrauterine foreign bodies on the incorporation of 35S into mucoprotein and of thymidine 2-14C into DNA of rat's endometrium.

    Science.gov (United States)

    Prager, R

    1969-01-01

    The metallic ions, copper and zinc, are extensively associated with biological processes. An imbalance in the normal concentration of these metals can alter the reproductive function. The experiments reported here were done to determine whether the insertion of a metallic device into the uterus of rats is associated with metabolic changes in the endometrium. For this purpose the effect of IUDs on the incorporation of 2Na 35S40 into the mucoproteins and of thymidine 2-carbon-14 into the DNA of the endometrium of normal rats was studied. Estradiol benzoate, 20 mcg, was injected im the day before instillation with radioisotopes in order to have all the rats under similar estrogenic stimulation. In Experiment 1 either .2 mc of 2Na35S40 or 2.5 mcc of thymidine 2-carbon-14 in .1 ml of distilled water was instilled into each uterus. To avoid expulsion of the isotope a ligature was placed in the lower portion of each horn. The opposite horn served as a control. The horns were excised at different intervals from 1 to 88 hours later. The results showed that the accumulation of radioactivity in the endometrium reaches its maximum concentration in about 22 hours and then gradually declines. In Experiment 2 copper wire, zinc wire, or nylon thread was used as an IUD. The opposite horn was used as a control. About 14 days later the isotope was instilled as in Experiment 1 and left for 24 hours. In the presence of an inert foreign body, such as nylon thread, a significant decrease in the accumulation of sulfur-35 in the endometrium (18.5%; p less than .05) was noted. With zinc wire the assimulation decrese was 44.2% (p less than .001), and with copper wire, it was 69.6% (p less than .001). Absorption of thymidine 2-carbon-14 was the same in both horns in the control group. Nylon thread produced no significant difference. Copper and zinc produced statistically significant depressions of absorption (p less than .001). Copper depression was greater than zinc depression (p less than .01). The nylon thread is considered to be an inert foreign body while the copper and zinc wires are active.

  6. Creep-Rupture Properties and Corrosion Behaviour of 21/4 Cr-1 Mo Steel and Hastelloy X-Alloys in Simulated HTGR Environment

    DEFF Research Database (Denmark)

    Lystrup, Aage; Rittenhouse, P. L.; DiStefano, J. R.

    Hastelloy X and 2/sup 1///sub 4/ Cr-1 Mo steel are being considered as structural alloys for components of a High-Temperature Gas-Cooled Reactor (HTGR) system. Among other mechanical properties, the creep behavior of these materials in HTGR primary coolant helium must be established to form part ...

  7. SU-E-T-214: Comparison of Treatment Techniques for Dupuytren’s Contracture and Risk Assessment of Normal Tissue Complications

    Energy Technology Data Exchange (ETDEWEB)

    Arentsen, L; Lopater, Z; Dusenbery, K; Gerbi, B [University of Minnesota, Minneapolis, MN (United States)

    2015-06-15

    Purpose: Duputren’s contracture (DC) is a benign disease characterized by abnormal thickening of the fascial surfaces of the hands or feet causing curling of the surface, functional impairment, weakness, and pain. The purpose of the investigation is to describe the radiation treatment approaches, compare these techniques, and discuss the potential side effects and complications of these techniques. Methods: Early stage DC has been treated with 120 kVp X rays but also with high-energy electrons or photons. High-energy electrons have been the radiation of choice but severe contracture of the hand makes it difficult to produce a plan with acceptable dose uniformity. High-energy photons can overcome this difficulty either by directing a beam onto the palmer or back of the surface of the hand, including bolus to maximize the surface dose. We calculated the dose to the bone for the 120 kVp treatment using published %DD data and mass energy absorption coefficients for bone and muscle. Results: The dose to underlying bone from megavoltage photons and electrons is essentially the same, but dose to the bone for using 120 kVp can be 4–5 times greater due to the photoelectric effect. For the 30 Gy dose deliver using this technique, the dose to the bone could be 84–105 Gy after taking the penetration of the beam into account. After radiotherapy, there is often decreased osteoblastic activity and vascular fibrosis that leads to osteitis, atrophy, and decreased metabolic bone activity. Incidence of fractures occurs routinely above 60 Gy with higher doses potentially leading to higher incidences of bone complications. Conclusion: Radiation therapy for DC using low-energy X rays can deliver a prohibitively high dose to the underlying bone potentially leading to severe bone complications.

  8. A data base and analysis program for shuttle main engine dynamic pressure measurements. Appendix C: Data base plots for SSME tests 902-214 through 902-314

    Science.gov (United States)

    Coffin, T.

    1986-01-01

    A dynamic pressure data base and data base management system developed to characterize the Space Shuttle Main Engine (SSME) dynamic pressure environment is reported. The data base represents dynamic pressure measurements obtained during single engine hot firing tests of the SSME. Software is provided to permit statistical evaluation of selected measurements under specified operating conditions. An interpolation scheme is included to estimate spectral trends with SSME power level. Flow Dynamic Environments in High Performance Rocket Engines are described.

  9. Poly[[μ2-1,4-bis(imidazol-1-ylmethylbenzene]bis(μ4-cyclohexane-1,4-dicarboxylatodicobalt(II

    Directory of Open Access Journals (Sweden)

    Qun-Di Yu

    2009-10-01

    Full Text Available In the title compound, [Co2(C8H10O42(C14H14N4]n, the two CoII atoms are both five-coordinated by four carboxylate O atoms, derived from two different cyclohexane-1,4-dicarboxylate (chdc ligands, and an N atom, derived from one end of a 1,4-bis(imidazol-1-ylmethylbenzene molecule (1,4-bix, in a distorted square-pyramidal environment. Each end of the chdc ligand links pairs of CoII atoms into a paddle-wheel assembly, i.e. Co2(O2CR′4; these are connected into rows because of the bridging nature of the chdc ligands, and the rows are further connected into a two-dimensional layer through the 1,4-bix ligands. The 1,4-bix ligand, which is disposed about a centre of inversion, is disorderd. Two positions were discerned for the –CH2(C6H4CH2– residue, with the major component having a site-occupancy factor of 0.512 (9.

  10. The Effect of Morphaction on the Kinetics of Indol-3yl-Acetic Acid-2-14C Transport in Zea Mays L. Coleoptile Segments

    DEFF Research Database (Denmark)

    Naqvi, S. M.

    1972-01-01

    This paper elucidates the effects of chloroflurenol (morphactin, IT 3456) treatment on the kinetics of 14C-IAA transport through Zea mays L. (cv. Orla-266) coleoptile segments. Maximum inhibition of transport was achieved when chloroflurenol remained in contact with the tissue segments for at least...

  11. Characterization and performance of UNS S63019 (21-4N) as bipolar plate material in a simulated polymer electrolyte membrane fuel cell environment

    Science.gov (United States)

    Howell, Andrew G.; Wang, Heli; Cowley, Scott W.; Turner, John A.

    The austenitic stainless steel UNS S63019 was evaluated regarding its potential as bipolar plate material in a polymer electrolyte membrane fuel cell (PEMFC) environment. Segregated grains of niobium carbide (NbC x) were identified in polished cross-sections of the alloy, offering a possible pathway for enhanced electrical conductivity through the passive surface oxide. Additionally, the alloy was tested for corrosion resistance in a simulated PEMFC environment. It was considered that perhaps the elevated nitrogen concentration in the alloy would provide some benefit for corrosion resistance. Results for interfacial contact resistance (ICR) testing of the air-formed surface film on UNS S63019 showed decreased electrical conductivity as compared to UNS S30400. Niobium carbide particles did not improve film conductivity due to a non-conductive niobium oxide layer that formed on the surface. Corrosion resistance of the alloy was also poor as compared with UNS S30400, demonstrating that elevated nitrogen concentration in the alloy was not adequate in itself to enhance corrosion resistance. Poor corrosion resistance was attributed primarily to high carbon content in the alloy which combined with a significant amount of chromium to form carbides.

  12. 100W class green 10ps 280µJ laser with M2<1.4 using Z-slab amplifier

    Science.gov (United States)

    Chard, Simon P.; Ramirez-Corral, Cristtel Y.; Bass, Michael; Chen, Ying; Kwon, Young K.

    2016-03-01

    A high-power green picosecond laser based on the `Z-slab' Nd:YAG amplifier is presented. The edge-pumped zigzag amplifier was designed to achieve high energy scaling with good beam quality. In a master oscillator power amplifier system 120 W average power was produced at 1064 nm, which was frequency doubled to 84 W at 532 nm. The maximum pulse energy was 400 μJ at 1064 nm and 280 μJ at 532 nm. The repetition rate was variable from 250 to 1000 kHz with M2 < 1.4. In burst mode with 1-10 pulses, over one millijoule total burst energy was demonstrated at 1064 nm.

  13. 20 CFR 30.214 - How does a claimant establish that the employee is a member of the Special Exposure Cohort?

    Science.gov (United States)

    2010-04-01

    ... February 1, 1992, at a gaseous diffusion plant located in Paducah, Kentucky; Portsmouth, Ohio; or Oak Ridge... one gaseous diffusion plant. (c) Proof of employment by the DOE or a DOE contractor, or an atomic... the plant of the external parts of the employee's body to radiation; or (ii) Worked in a job that...

  14. A pediatric bone mass scan has poor ability to predict adult bone mass: a 28-year prospective study in 214 children.

    Science.gov (United States)

    Buttazzoni, Christian; Rosengren, Bjorn E; Tveit, Magnus; Landin, Lennart; Nilsson, Jan-Åke; Karlsson, Magnus K

    2014-02-01

    As the correlation of bone mass from childhood to adulthood is unclear, we conducted a long-term prospective observational study to determine if a pediatric bone mass scan could predict adult bone mass. We measured cortical bone mineral content (BMC [g]), bone mineral density (BMD [g/cm(2)]), and bone width (cm) in the distal forearm by single photon absorptiometry in 120 boys and 94 girls with a mean age of 10 years (range 3-17) and mean 28 years (range 25-29) later. We calculated individual and age-specific bone mass Z scores, using the control cohort included at baseline as reference, and evaluated correlations between the two measurements with Pearson's correlation coefficient. Individual Z scores were also stratified in quartiles to register movements between quartiles from growth to adulthood. BMD Z scores in childhood and adulthood correlated in both boys (r = 0.35, p pediatric bone scan with a value in the lowest quartile had a sensitivity of 48% (95% confidence interval [CI] 27-69%) and a specificity of 76% (95% CI 66-84%) to identify individuals who would remain in the lowest quartile also in adulthood. Childhood forearm BMD explained 12% of the variance in adult BMD in men and 25% in women. A pediatric distal forearm BMD scan has poor ability to predict adult bone mass.

  15. “晶”言万语 我爱你——“2.14”水晶之旅

    Institute of Scientific and Technical Information of China (English)

    Zee

    2010-01-01

    它是时间的记载,用来记录生命中最美好的瞬间;它是你我之间的"红线",用来倾诉彼此的诺言……2月14日,我们相约水晶之旅,在道不尽的"晶"言万语中诉说"我爱你"!

  16. IN VIVO HUMAN METABOLISM OF [2-14C]2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE (PHIP). (R825280)

    Science.gov (United States)

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  17. THE DETECTION OF [2-14C]2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE METABOLITES IN HUMANS. (R825280)

    Science.gov (United States)

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  18. Crystal structure of 2-{[1-(4-bromobenzyl-1H-1,2,3-triazol-4-yl]methoxy}naphthalene-1,4-dione

    Directory of Open Access Journals (Sweden)

    Rajamani Raja

    2015-04-01

    Full Text Available In the title compound, C20H14BrN3O3, the benzene ring makes dihedral angles of 71.30 (11 and 68.95 (14° with the naphthalene ring system and the triazole ring, respectively. The latter two ring systems are coplanar, with a dihedral angle of 2.92 (12°. The O atoms deviate from the naphthalene ring system by 0.029 (2 and −0.051 (2 Å. In the crystal, molecules are linked by C—H...O and C—H...N hydrogen bonds, forming ribbons parallel to (10-1. The ribbons are linked via C—H...O and π–π stacking interactions [centroid–centroid distance = 3.4451 (14 Å], forming slabs parallel to the bc plane.

  19. 10 CFR Appendix E to Part 20 - Nationally Tracked Source Thresholds

    Science.gov (United States)

    2010-01-01

    ... Category 1(TBq) Category 1(Ci) Category 2(TBq) Category 2(Ci) Actinium-227 20 540 0.2 5.4 Americium-241 60... 2 54 Strontium-90 1,000 27,000 10 270 Thorium-228 20 540 0.2 5.4 Thorium-229 20 540 0.2 5.4...

  20. I. The metabolic properties of plutonium and allied materials

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, J.G.

    1948-05-24

    This report on the metabolic properties of plutonium and related radioactive materials presents experimental information in the following areas: radioautographic studies; tracer studies (with tables of accumulation in tissues) of actinium, radio-zirconium, technetium, radio-rubidium, radio-germanium, beryllium, and cadmium; decontamination and bone metabolism studies; and radio-chemical isolation.

  1. Monofunctionalization of Calix[4]arene Tetracarboxylic Acid at the Upper Rim with Isothiocyanate Group: First Bifunctional Chelating Agent for Alpha-Emitter Ac-225.

    Science.gov (United States)

    Chen, Xiaoyuan; Ji, Min; Fisher, Darrell R; Wai, Chien M

    1999-09-01

    A procedure is reported for synthesizing a novel, water-soluble bifunctional chelating agent derived from calix[4]arene. This chelate features tetracarboxylic acid groups at the lower rim as an actinium-225 ionophore, and an isothiocyanate functional group at the upper rim for labeling of the N-terminus of monoclonal antibodies through thiourea linkage.

  2. The influence of children by product placement advertising: Case study on 214 students of Shunhe School in Liuan city%广告植入方式和曝光效应对儿童认知的影响——对六安顺河希望小学214名学生的实验调查

    Institute of Scientific and Technical Information of China (English)

    宋思根; 陈翠

    2012-01-01

    传播环境的变化迫使人们不断挖掘广告资源,植入式广告深受业界瞩目,而其对受众认知的影响机制十分复杂.以儿童为研究对象,数据显示儿童对外显整合植入广告的正确回忆率和识别率均高于内隐整合植入广告,但没有统计意义上的显著性;植入式广告回忆和识别受儿童信息处理能力的影响十分显著,提示处理人对植入信息的正确记忆率和广告意图识别率显著高于受限处理人;植入式广告的内隐记忆对儿童的购买行为存在启动效应,在受到重复刺激时尤为显著.

  3. Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation

    Directory of Open Access Journals (Sweden)

    Vanessa de Cássia Teixeira da Silva

    2016-01-01

    Full Text Available This work reports endoglucanase and beta-glucosidase production by the thermophilic fungus Myceliophthora heterothallica in solid-state (SSC and submerged (SmC cultivation. Wheat bran and sugarcane bagasse were used for SSC and cardboard for SmC. Highest endoglucanase production in SSC occurred after 192 hours: 1,170.6 ± 0.8 U/g, and in SmC after 168 hours: 2,642 ± 561 U/g. The endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their optimal pH and temperature. The number of endoglucanases was also different: six isoforms in SSC and ten in SmC. Endoglucanase activity remained above 50% after incubation between pH 3.0 and 9.0 for 24 h for both cultivation systems. The effect of several chemicals displayed variation between SSC and SmC isoenzymes. Manganese activated the enzymes from SmC but inhibited those from SSC. For β-glucosidases, maximum production on SmC was 244 ± 48 U/g after 168 hours using cardboard as carbon source. In SSC maximum production reached 10.9 ± 0.3 U/g after 240 h with 1 : 1 wheat bran and sugarcane bagasse. Manganese exerted a significant activation on SSC β-glucosidases, and glucose inhibited the enzymes from both cultivation systems. FeCl3 exerted the strongest inhibition for endoglucanases and β-glucosidases.

  4. [Deamination and reductive deamination of (2-amino-5, 6-dimethylbenzimidazolyl)cobamide. Preparation of (2-hydroxy-5, 6-dimethylbenzimidazolyl)cobamide and (5, 6-dimethyl-[2(-14)C]-benzimidazolyl)cobamide].

    Science.gov (United States)

    Fridrich, E; Müller, O

    1984-02-01

    (2-Amino-5, 6-dimethylbenzimidazolyl)-cobamide (III) is transformed to (2-hydroxy-5, 6-dimethylbenzimidazolyl) cobamide (IV) by nitrous acid. Exchange of the NH2-group by hydrogen with nitrous acid/hypophosphorous acid yields vitamin B12 (I). This reaction completes a cycle vitamin B12 (I)----[carboxy(2-cyanoamino-4,5-dimethylphenyl)amino]cobamide+ ++ (II)----(2-amino-5,6-dimethylbenzimidazolyl)cobamide (III)----vitamin B12 (I), which allows chemical 14C-labelling of vitamin B12. In this procedure cyanogen bromide, which is necessary for the first step, was labelled with [14C] cyanide. By the following reactions a vitamin B12 was formed in which C-2 of the 5, 6-dimethylbenzimidazole moiety is labelled.

  5. Crystal structure of poly[[{μ2-1,4-bis[(1H-imidazol-1-ylmethyl]benzene}[μ6-5-(4-carboxylatophenoxyisophthalato]-μ3-hydroxido-dicobalt(II] 0.25-hydrate

    Directory of Open Access Journals (Sweden)

    Yaping Li

    2014-11-01

    Full Text Available The title coordination polymer, {[Co2(C15H7O7(OH(C14H14N4]·0.25H2O}n, was synthesized under hydrothermal conditions. The asymmetric unit contains two Co2+ ions, one L3− anion originating from 5-(4-carboxyphenoxyisophthalic acid (H3L, one OH− ligand, one 1,4-bis[(1H-imidazol-l-ylmethyl]benzene (bix ligand and one disordered lattice water molecule (occupancy 0.25. The two Co2+ ions have different environments. One has an octahedral O4N2 coordination sphere, defined by four O atoms from three carboxylate groups and one OH− ligand, and two N atoms from two symmetry-related bix ligands. The other has a trigonal-bipyramidal O5 coordination sphere resulting from three carboxylate groups and two OH− ligands. The dihedral angles between the two benzene rings in the L3− ligand and between the benzene ring and the two imidazole rings in the bix ligand are 67.05 (15, 75.27 (17 and 82.05 (17°, respectively. Four neighbouring Co2+ ions are linked by six carboxylate groups and two μ3-OH ligands, forming a butterfly-shaped secondary building unit (SBU. These SBUs are connected by L3− anions into layers parallel to (1-10. Adjacent layers are cross-linked by the bix ligands, forming a three-dimensional framework that has a bimodal (3,8-connected tfz-d topology. The disordered lattice water molecule is located in the voids of the framework and has O...O and O...N contacts of 2.81 (2 and 2.95 (2 Å, suggesting medium-strength hydrogen bonds. The title compound may be a good candidate for artificial eye lenses.

  6. Ethyl 2-[1-(4-chlorophenyl-2,5-dioxo-1,2,3,5-tetrahydroimidazo[1′,2′:1,2]pyrimidino[5,4-b][1]benzofuran-3-yl]acetate

    Directory of Open Access Journals (Sweden)

    Yang-Gen Hu

    2008-01-01

    Full Text Available The asymmetric unit of the title compound, C22H16ClN3O5, consists of two crystallographically independent molecules. The fused rings of the imidazo[1,2-a]benzo[4,5]furo[3,2-d]pyrimidine system are nearly coplanar and the chlorophenyl rings are twisted with respect to the two pyrimidinone ring systems by 71.00 (2 and 62.59 (2°. The C atoms of the ethyl side chain are disordered and were refined using a split model. In the crystal structure, the molecules are connected via weak intra- and intermolecular C—H...O interactions are present. The ethyl group in one molecule is disordered over two positions, with site occupancy factors 0.55 and 0.45; in the other molecule only the methyl group is disordered over two positions, with site occupancy factors 0.6 and 0.4.

  7. Short-term and long-term outcomes of single-incision versus multi-incision laparoscopic resection for colorectal cancer: a propensity-score-matched analysis of 214 cases.

    Science.gov (United States)

    Katsuno, Goutaro; Fukunaga, Masaki; Nagakari, Kunihiko; Yoshikawa, Seichiro; Azuma, Daisuke; Kohama, Shintaro

    2016-04-01

    The aim of this study was to reveal the short-term and long-term outcomes of single-incision laparoscopic colorectal resection (SILC) compared with multi-incision laparoscopic colorectal resection (MILC) for colorectal cancer using propensity score matching analysis. The study group included 235 patients who underwent SILC and 730 patients who underwent MILC for colorectal cancer between April 2009 and September 2014. The propensity score matching for age, gender, body mass index, tumor location (right-sided colon/sigmoid colon/upper rectum), lymph node dissection (D1/D2/D3), pathologic T (≤T3) stage and TNM (0-I/II/III) stage produced 107 matched pairs. The exclusion criteria for SILC were as follows: (1) tumors located at the transverse, descending colon or lower rectum, (2) stage IV tumors, synchronous or previous malignancies, (3) locally advanced tumors >T4, (4) acute obstructions or previous major abdominal surgery and (5) obese patients: BMI > 30. No significant differences were observed in operating time, bleeding volumes, starting time of liquid diet and length of hospital stay between the SILC and MILC groups. However, the SILC group showed less analgesic requirements (1.1 vs. 1.9 times; p = 0.0006) and shorter length of incision (2.7 vs. 4.3 cm; p = 0.0000) compared to MILC group. The overall rate of postoperative complications was similar in both groups (2.8 vs. 3.7%, p = 0.70). The 5-year overall survival rate of SILC and MILC was 100 and 95% (p = 0.125) and 5-year disease-free survival rates in stages 0-III were 97 and 94% (p = 0.189), 100 and 92% in stage II and 90 and 85% in stage III, respectively. This study suggests that SILC for colorectal cancer is a safe and feasible option with better cosmetic results and less pain in strictly selected patients. SILC can also produce good oncological results with similar postoperative outcomes to MILC.

  8. Poly[[(μ2-benzene-1,3-di-carboxyl-ato){μ2-1,4-bis-[(1H-imidazol-1-yl)meth-yl]benzene}-cadmium] di-methyl-formamide monosolvate].

    Science.gov (United States)

    Chen, Yan; Wang, Daguang; He, Liang; Li, Wei; Suo, Jian

    2013-10-23

    The title coordination polymer, {[Cd(C8H4O4)(C14H14N4)]·C3H7NO} n , was synthesized by solvothermal reaction of metallic cadmium with the semi-rigid neutral ligand 1,4-bis-[(1H-imidazol-1-yl)meth-yl]benzene (bix) and the V-shaped benzene-1,3-di-carb-oxy-lic acid (m-H2bdc). The structure exhibits a pseudo-C-centring which is almost fulfilled by the polymeric metal complex but not by the solvent dimethylform-amide (DMF) mol-ecules. The asymmetric unit contains two independent Cd(II) ions, two m-bdc(2-) ligands, one and two half bix ligands, and two solvent DMF mol-ecules. The Cd(II) ions are both five-coordinated by three O atoms from two different m-bdc(2-) ligands and two N atoms from two different bix ligands in a distorted square-pyramidal geometry. The m-bdc(2-) ligands adopt a chelate-monodentate coordination mode, connecting neighboring Cd(II) ions into a zigzag chain parallel to [110]. Adjacent chains are further cross-linked by bix ligands, giving rise to a puckered sheet nearly perpendicular to the chain direction. Thus, each Cd(II) ion is connected to four neighboring Cd(II) ions through two m-bdc(2-) anions and two bix ligands, giving rise to the final non-inter-penetrating uninodal layer with sql (4,4) topology.

  9. Cai Hua, L’homme pensé par l’homme, Du statut scientifique des sciences sociales Paris, PUF, 2008, 214 p. et Laurent Barry, La parenté, Paris, Gallimard, 2008, 567 p.

    OpenAIRE

    2009-01-01

    Deux ouvrages récents réintroduisent la Chine dans l’anthropologie de la parenté. Cai Hua et Laurent Barry sont tous deux des élèves de Françoise Héritier, qui a renouvelé au Collège de France la connaissance des systèmes de parenté africains, et leurs ouvrages, construits de façon parallèle, accordent une grande place aux systèmes Han. L’anthropologie de la parenté, fondée en 1870 par l’ouvrage de Lewis Henry Morgan, Systems of Consanguinity and Affinity of the Human Family, et relancée en 1...

  10. Interferon regulatory factor-1 (IRF-1) is involved in the induction of phosphatidylserine receptor (PSR) in response to dsRNA virus infection and contributes to apoptotic cell clearance in CHSE-214 cell.

    Science.gov (United States)

    Kung, Hsin-Chia; Evensen, Øystein; Hong, Jiann-Ruey; Kuo, Chia-Yu; Tso, Chun-Hsi; Ngou, Fang-Huar; Lu, Ming-Wei; Wu, Jen-Leih

    2014-10-23

    The phosphatidylserine receptor (PSR) recognizes a surface marker on apoptotic cells and initiates engulfment. This receptor is important for effective apoptotic cell clearance and maintains normal tissue homeostasis and regulation of the immune response. However, the regulation of PSR expression remains poorly understood. In this study, we determined that interferon regulatory factor-1 (IRF-1) was dramatically upregulated upon viral infection in the fish cell. We observed apoptosis in virus-infected cells and found that both PSR and IRF-1 increased simultaneously. Based on a bioinformatics promoter assay, IRF-1 binding sites were identified in the PSR promoter. Compared to normal viral infection, we found that PSR expression was delayed, viral replication was increased and virus-induced apoptosis was inhibited following IRF-1 suppression with morpholino oligonucleotides. A luciferase assay to analyze promoter activity revealed a decreasing trend after the deletion of the IRF-1 binding site on PSR promoter. The results of this study indicated that infectious pancreatic necrosis virus (IPNV) infection induced both the apoptotic and interferon (IFN) pathways, and IRF-1 was involved in regulating PSR expression to induce anti-viral effects. Therefore, this work suggests that PSR expression in salmonid cells during IPNV infection is activated when IRF-1 binds the PSR promoter. This is the first report to show the potential role of IRF-1 in triggering the induction of apoptotic cell clearance-related genes during viral infection and demonstrates the extensive crosstalk between the apoptotic and innate immune response pathways.

  11. Study on preservatives of vacuum-packed Chinese New Year rice cake . Journal of Zhejiang University (Agric . & Life Sci .), 2013,39(2):209-214%真空包装年糕保鲜剂研究

    Institute of Scientific and Technical Information of China (English)

    张希; 何逸波; 杜鹃; 冯凤琴

    2013-01-01

    Summary Water milled Chinese New Year rice cake ( NYRC ) is a kind of delicious and nutritive Chinese traditional food . But the short shelf life of the NYRC restricts its industrialization development . The purpose of the study is to inhibit the growth of spoilage microorganisms effectively and to extend the shelf life of the vacuum‐packed NYRC . There were four main steps:1) the spoilage microorganisms were separated and characterized from vacuum‐packed NYRC . 2) Six commonly used antibacterial agents were chosen against the spoilage microorganisms , and the minimum inhibitory concentrations ( MIC ) were determined . 3 ) The synergistic effects between glycerol monolaurate ( GML) and other preservatives were measured . 4) In order to investigate application effect of chosen food preservatives , lab and factory testes were taken in NYRC product . The moisture , hardness and colour ( L value) of the vacuum‐packed NYRC were determined . The main results were as follows:1) The spoilage microorganisms which led to corruption of vacuum‐packed NYRC were two kinds of Bacillus . 2) Among the tested preservatives , GML had the lowest MIC which was 32μg/mL . 3 ) There was synergistic effect of GML and sodium dehydroacetate , and the fractional inhibitory concentration index ( FIC I ) was 0 .531 . The compound preservative made up of GML , citric acid and sodium dehydroacetate at the mass ratio of 1∶1∶1 was selected against the Bacillus . 4) Adding the selected compound preservative of 1 .5g/kg could extend the shelf life from about 7 d to 20 d at 37 ℃ . The compound preservative not only had no adverse effect on the moisture and color of NYRC , but also could reduce the hardness efficiently . In NYRC products , the compound preservative shows significantly better effect than the monomer GML . In addition , while prolonging the shelf life of the vacuum‐packed NYRC , the compound preservative had no adverse effect on its quality .%  为了有效地抑制真空包装水磨年糕腐败微生物的生长,延长其保质期,首先分离并鉴定真空包装年糕中的腐败微生物;针对该菌种选取相应的保鲜剂,并通过测定单体和复配保鲜剂的最低抑制质量浓度和协同增效作用,确定适用于真空包装年糕的复配保鲜剂.结果表明:导致真空包装年糕腐败的微生物主要为芽孢杆菌属( Bacillus);针对该类微生物的适宜复配保鲜剂配方为 w(单甘油月桂酸酯)∶ w(柠檬酸)∶ w(脱氢醋酸钠)=1∶1∶1;经过实验室和工厂大试检测表明,添加1.5 g/kg该复配物,同时在95℃灭菌60 min ,能将真空包装水磨年糕的保质期由原来在37℃下7 d左右提高到20 d .说明用该配方不仅能有效地延长年糕的保质期,且其品质不会受到影响;单甘油月桂酸酯还可降低年糕的硬度.

  12. A modern approach to the synthesis of 2-(4-chlorophenyl)[2-(14)C]thiazol-4-ylacetic acid ([(14)C] fenclozic acid) and its acyl glucuronide metabolite.

    Science.gov (United States)

    Killick, David A; Bushby, Nick

    2013-01-01

    An updated approach to the 1960s synthesis of [(14)C] fenclozic acid from labelled potassium cyanide is presented. By employing modern synthetic methodology and purification techniques, many of the inherent hazards in the original synthesis are avoided or significantly reduced. The concomitant labelled stereoselective synthesis of the key acyl glucuronide metabolite (the 1-β-O-acyl glucuronide) is also described.

  13. American Recovery and Reinvestment Act (ARRA) FEMP Technical Assistance U.S. Army – Project 214 Analysis of Regulations Associated with Implementation of a Rocky Mountain Secure Smart-Grid

    Energy Technology Data Exchange (ETDEWEB)

    Warwick, William M.

    2010-09-30

    This document describes technical assistance provided by PNNL to further develop a smart grid technologies concept to provide a basis for policies and plans for the US Army. The effort was to analyze the potential to utilize emerging smart grid technologies along with indigenous renewable and other resources to meet the emergency and other power needs of Department of Defense facilities in Colorado and Wyoming.

  14. 2-[1-(4-Bromo­phen­yl)-3-hy­droxy-3-(4-meth­oxy­phen­yl)prop­yl]cyclo­hexa­nol

    Science.gov (United States)

    Çelik, Ísmail; Akkurt, Mehmet; Gezegen, Hayreddin; Üremiş, Muhammed M.; Duteanu, Narcis

    2013-01-01

    In the title compound, C22H27BrO3, the cyclo­hexane ring adopts a chair conformation. The dihedral angle between the benzene rings is 41.9 (4)°. In the crystal, mol­ecules are linked by O—H⋯O and C—H⋯O hydrogen bonds, forming a three-dimensional network. In addition, π–π stacking inter­actions [centroid–centroid distance = 3.953 (6) Å] between the benzene rings of the meth­oxy­benzene groups occur. PMID:24046654

  15. Poly[bis[μ2-1,4-bis(1,2,4-triazol-1-ylmethylbenzene-κ2N4:N4′]bis(nitrito-κOcobalt(II

    Directory of Open Access Journals (Sweden)

    Xia Zhu

    2010-01-01

    Full Text Available The CoII atom in the title complex, [Co(NO22(C12H12N62]n, lies on an inversion center and is coordinated by four N atoms from the triazole rings of two symmetry-related pairs of 1,4-bis(1,2,4-triazol-1-ylmethylbenzene (bbtz ligands and two O atoms from two symmetry-related monodentate nitrate ligands in a distorted octahedral geometry. The Co atoms are bridged by four bbtz ligands, forming a two-dimensional (4,4 network parallel to (102.

  16. Two double-blinded, randomized, comparative trials of 4 human immunodeficiency virus type 1 (HIV-1) envelope vaccines in HIV-1-infected individuals across a spectrum of disease severity: AIDS Clinical Trials Groups 209 and 214.

    Science.gov (United States)

    Schooley, R T; Spino, C; Kuritzkes, D; Walker, B D; Valentine, F A; Hirsch, M S; Cooney, E; Friedland, G; Kundu, S; Merigan, T C; McElrath, M J; Collier, A; Plaeger, S; Mitsuyasu, R; Kahn, J; Haslett, P; Uherova, P; deGruttola, V; Chiu, S; Zhang, B; Jones, G; Bell, D; Ketter, N; Twadell, T; Chernoff, D; Rosandich, M

    2000-11-01

    The potential role of human immunodeficiency virus type 1 (HIV-1)-specific immune responses in controlling viral replication in vivo has stimulated interest in enhancing virus-specific immunity by vaccinating infected individuals with HIV-1 or its components. These studies were undertaken to define patient populations most likely to respond to vaccination, with the induction of novel HIV-1-specific cellular immune responses, and to compare the safety and immunogenicity of several candidate recombinant HIV-1 envelope vaccines and adjuvants. New lymphoproliferative responses (LPRs) developed in 350 cells/mm(3) and were usually strain restricted. Responders tended to be more likely than nonresponders to have an undetectable level of HIV-1 RNA at baseline (P=.067). Induction of new cellular immune responses by HIV-1 envelope vaccines is a function of the immunologic stage of disease and baseline plasma HIV-1 RNA level and exhibits considerable vaccine strain specificity.

  17. [Rezension zu:] Ján Demčišák: Queer Reading von Brechts Frühwerk. Marburg: Tectum Verlag, 2012. 214 S. ISBN 978-3-8288-2995-4

    OpenAIRE

    Gáfrik, Róbert

    2016-01-01

    Über Bertolt Brecht wurde schon viel geschrieben. Die Menge der Literatur, die Brecht und sein Werk als Hauptthema behandelt oder auf sie nur flüchtig eingeht, ist immens. Brechts Werk wurde schon von verschiedenen Aspekten analysiert und interpretiert. Ján Demčišáks Buch ist aber kein weiteres Buch über Brecht, das bekanntes Wissen verkauft. Es ist nämlich Demčišák gelungen, eine Nische in der fast unüberschaubaren Brechtforschung zu finden, die Brechts frühes Werk erfrischend interpretiert ...

  18. Hyperspectral surface materials map of quadrangles 3666 and 3766, Balkh (219), Mazar-e Sharif (220), Qarqin (213), and Hazara Toghai (214) quadrangles, Afghanistan, showing iron-bearing minerals and other materials

    Science.gov (United States)

    King, Trude V.V.; Hoefen, Todd M.; Kokaly, Raymond F.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected iron-bearing minerals and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. This map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Goethite and jarosite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  19. Hyperspectral surface materials map of quadrangles 3666 and 3766, Balkh (219), Mazar-e Sharif (220), Qarqin (213), and Hazara Toghai (214) quadrangles, Afghanistan, showing carbonates, phyllosilicates, sulfates, altered minerals, and other materials

    Science.gov (United States)

    Kokaly, Raymond F.; King, Trude V.V.; Hoefen, Todd M.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected carbonates, phyllosilicates, sulfates, altered minerals, and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. The map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Epidote or chlorite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  20. The alternative lengthening of telomere phenotype is significantly associated with loss of ATRX expression in high-grade pediatric and adult astrocytomas: a multi-institutional study of 214 astrocytomas.

    Science.gov (United States)

    Abedalthagafi, Malak; Phillips, Joanna J; Kim, Grace E; Mueller, Sabine; Haas-Kogen, Daphne A; Marshall, Roxanne E; Croul, Sidney E; Santi, Mariarita R; Cheng, Jing; Zhou, Shengmei; Sullivan, Lisa M; Martinez-Lage, Maria; Judkins, Alexander R; Perry, Arie

    2013-11-01

    Loss-of-function of alpha thalassemia/mental retardation syndrome X-linked (ATRX) protein leads to a phenotype called alternative lengthening of telomeres (ALT) in some tumors. High-grade astrocytomas comprise a heterogeneous group of central nervous system tumors. We examined a large cohort of adult (91) and pediatric (n=88) high-grade astrocytomas as well as lower grade forms (n=35) for immunohistochemical loss of ATRX protein expression and the presence of ALT using telomere-specific fluorescence in situ hybridization, with further correlation to other known genetic alterations. We found that in pediatric high-grade astrocytomas, 29.6% of tumors were positive for ALT and 24.5% were immunonegative for the ATRX protein, these two alterations being highly associated with one another (PATRX protein immunonegative cases (PATRX protein expression in our adult high-grade astrocytomas. In both cohorts, however, the ALT positive high-grade astrocytomas showed more frequent amplification of the platelet-derived growth factor receptor alpha gene (PDGFRA; 45% and 50%, respectively) than the ALT negative counterparts (18% and 26%; P=0.03 for each). In summary, our data show that the ALT and ATRX protein alterations are common in both pediatric and adult high-grade astrocytomas, often with associated PDGFRA gene amplification.

  1. Study of the origin of elements of the uranium-235 family observed in excess in the vicinity of the experimental nuclear EL4 reactor under dismantling. Lessons got at this day and conclusions; Etude de l'origine des elements de la famille de l'uranium-235 observes en exces dans les environs du reacteur nucleaire experimental EL4 en cours de demantelement. Enseignements retires a ce jour et conclusion

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This study resumes the discovery of an excess of actinium 227 found around by EL4 nuclear reactor actually in dismantling. The search for the origin of this excess revealed a real inquiry of investigation during three years. Because a nuclear reactor existed in this area a particular attention will have concerned this region. The doubt became the line of conduct to find the answer to the human or natural origin of this excess. Finally and against any evidence, it appears that the origin of this phenomenon was natural, consequence of the particular local geology. The detail of the different investigations is given: search of a possible correlation with the composition of elevations constituent of lanes, search (and underlining) of new sites in the surroundings of the Rusquec pond and the Plouenez station, study of the atmospheric deposits under winds of the nuclear power plant and in the east direction, search of a possible relationship with the gaseous effluents of the nuclear power plant in the past, historical study of radioactive effluents releases in the fifty last years by the analysis of the sedimentary deposits in the Saint-Herbiot reservoir, search of a possible correlation between the excess of actinium 227 and the nuclear power plant activity; search of a possible correlation with a human activity without any relationship with the nuclear activities, search of a correlation with the underground waters, search of a correlation with the geological context, collect of information on the possible transfers in direction of the food chain, determination of the radiological composition of the underground waters ( not perturbed by human activity), search of the cause of an excess of actinium 227 in the old channel of liquid effluents release of the nuclear power plant. The results are given and discussed. And contrary to all expectations the origin of the excess of actinium 227 is completely natural. (N.C.)

  2. Behaviour of uranium series radionuclides in surface water (Crouzille, Limousin). Geochemical implications; Comportement des radionucleides des familles de l'uranium dans les eaux superficielles du site de la Crouzille (Limousin): implications geochimiques

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-06-15

    Understanding natural radionuclides behaviour in surface water is a required step to achieve uranium mine rehabilitation and preserve water quality. The first objective of this thesis is to determine which are the radionuclides sources in a drinking water reservoir. The second objective is to improve the knowledge about the behaviour of uranium series radionuclides, especially actinium. The investigated site is a brook (Sagnes, Limousin, France) which floods a peat bog contaminated by a former uranium mine and which empties into the Crouzille lake. It allows studying radionuclides transport in surface water and radionuclides retention through organic substance or water reservoir. Radionuclides distribution in particulate, colloidal and dissolved phases is determined thanks to ultra-filtrations. Gamma spectrometry allows measuring almost all natural radionuclides with only two counting stages. However, low activities of {sup 235}U series radionuclides impose the use of very low background well-type Ge detectors, such as those of the Underground Laboratory of Modane (France). Firstly, this study shows that no or few radionuclides are released by the Sagnes peat bog, although its radioactivity is important. Secondly, it provides details on the behaviour of uranium series radionuclides in surface water. More specifically, it provides the first indications of actinium solubility in surface water. Actinium's behaviour is very close to uranium's even if it is a little less soluble. (author)

  3. 49 CFR 585.76 - Reporting requirements.

    Science.gov (United States)

    2010-10-01

    ... years as complying with the upgraded moving deformable barrier (S7.2 of Standard No. 214)(49 CFR 571.214... concerning its compliance with the moving deformable barrier requirements of S7 of Standard No. 214 and with... meeting the moving deformable barrier test requirements of S7.2 of Standard No. 214, Side...

  4. DISSOLVED CONCENTRATION LIMITS OF RADIOACTIVE ELEMENTS

    Energy Technology Data Exchange (ETDEWEB)

    P. Bernot

    2005-07-13

    The purpose of this study is to evaluate dissolved concentration limits (also referred to as solubility limits) of elements with radioactive isotopes under probable repository conditions, based on geochemical modeling calculations using geochemical modeling tools, thermodynamic databases, field measurements, and laboratory experiments. The scope of this activity is to predict dissolved concentrations or solubility limits for elements with radioactive isotopes (actinium, americium, carbon, cesium, iodine, lead, neptunium, plutonium, protactinium, radium, strontium, technetium, thorium, and uranium) relevant to calculated dose. Model outputs for uranium, plutonium, neptunium, thorium, americium, and protactinium are provided in the form of tabulated functions with pH and log fCO{sub 2} as independent variables, plus one or more uncertainty terms. The solubility limits for the remaining elements are either in the form of distributions or single values. Even though selection of an appropriate set of radionuclides documented in Radionuclide Screening (BSC 2002 [DIRS 160059]) includes actinium, transport of Ac is not modeled in the total system performance assessment for the license application (TSPA-LA) model because of its extremely short half-life. Actinium dose is calculated in the TSPA-LA by assuming secular equilibrium with {sup 231}Pa (Section 6.10); therefore, Ac is not analyzed in this report. The output data from this report are fundamental inputs for TSPA-LA used to determine the estimated release of these elements from waste packages and the engineered barrier system. Consistent modeling approaches and environmental conditions were used to develop solubility models for the actinides discussed in this report. These models cover broad ranges of environmental conditions so they are applicable to both waste packages and the invert. Uncertainties from thermodynamic data, water chemistry, temperature variation, and activity coefficients have been quantified or

  5. Transition wavelengths and unresolved transition array statistics of ions with Z = 72-89

    Energy Technology Data Exchange (ETDEWEB)

    Kilbane, D, E-mail: Deirdre.Kilbane@ucd.ie [School of Physics, University College Dublin, Belfield, Dublin 4 (Ireland)

    2011-08-28

    Potential extreme ultraviolet and soft x-ray radiation sources have been identified, using the flexible atomic code (FAC), as emission peaks arising from the 4d-4f and 4p-4d transitions in Pd-like to Rb-like ions of hafnium through actinium. The effects of configuration interaction are investigated and for increasing nuclear charge, these strong emitters are seen to separate and move to shorter wavelengths. Each source is characterized using the unresolved transition array model. They are proposed to complement the currently used nitrogen and argon sources in the 'water window', and as possible successors to tin in next-generation lithography.

  6. Detection of rare earth elements in Powder River Basin sub-bituminous coal ash using laser-induced breakdown spectroscopy (LIBS)

    Energy Technology Data Exchange (ETDEWEB)

    Tran, Phuoc [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State; Mcintyre, Dustin [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State

    2015-10-01

    We reported our preliminary results on the use of laser-induced breakdown spectroscopy to analyze the rare earth elements contained in ash samples from Powder River Basin sub-bituminous coal (PRB-coal). We have identified many elements in the lanthanide series (cerium, europium, holmium, lanthanum, lutetium, praseodymium, promethium, samarium, terbium, ytterbium) and some elements in the actinide series (actinium, thorium, uranium, plutonium, berkelium, californium) in the ash samples. In addition, various metals were also seen to present in the ash samples

  7. Analysis of the Yrast Bands with q-Deformed Moment of Inertia

    Institute of Scientific and Technical Information of China (English)

    FANG Xiang-Zheng; RUAN Tu-Nan

    2001-01-01

    The rigid-rotor with a q-deformed moment of inertia is introduced to describe the nuclear rotational spectra. With the representations of quantum algebra, the normal deformed and superdeformed bands are naturally differentiated by softness. The yrast normal deformed bands in rare earth and actinium regions, as well as the yrast superdeformed bands in A-190 and 150 regions are investigated. The calculated results agree with experimental data qualitatively well, and the values of the parameters are physically reasonable. This indicates that the fixed deformation, the stretching effect and the many body statistics effect are three possible dominant factors to govern nuclear rotational bands.

  8. Gclust Server: 48009 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 48009 Bsu_BSU23530=spoIIM Cluster Sequences Related Sequences(8) 214 required for dissolution...quences Related Sequences(8) Sequence length 214 Representative annotation required for dissolution

  9. Purification of cerium, neodymium and gadolinium for low background experiments

    Directory of Open Access Journals (Sweden)

    Boiko R.S.

    2014-01-01

    Full Text Available Cerium, neodymium and gadolinium contain double beta active isotopes. The most interesting are 150Nd and 160Gd (promising for 0ν2β search, 136Ce (2β+ candidate with one of the highest Q2β. The main problem of compounds containing lanthanide elements is their high radioactive contamination by uranium, radium, actinium and thorium. The new generation 2β experiments require development of methods for a deep purification of lanthanides from the radioactive elements. A combination of physical and chemical methods was applied to purify cerium, neodymium and gadolinium. Liquid-liquid extraction technique was used to remove traces of Th and U from neodymium, gadolinium and for purification of cerium from Th, U, Ra and K. Co-precipitation and recrystallization methods were utilized for further reduction of the impurities. The radioactive contamination of the samples before and after the purification was tested by using ultra-low-background HPGe gamma spectrometry. As a result of the purification procedure the radioactive contamination of gadolinium oxide (a similar purification efficiency was reached also with cerium and neodymium oxides was decreased from 0.12 Bq/kg to 0.007 Bq/kg in 228Th, from 0.04 Bq/kg to <0.006 Bq/kg in 226Ra, and from 0.9 Bq/kg to 0.04 Bq/kg in 40K. The purification methods are much less efficient for chemically very similar radioactive elements like actinium, lanthanum and lutetium.

  10. Large scale accelerator production of (225)Ac: Effective cross sections for 78-192MeV protons incident on (232)Th targets.

    Science.gov (United States)

    Griswold, J R; Medvedev, D G; Engle, J W; Copping, R; Fitzsimmons, J M; Radchenko, V; Cooley, J C; Fassbender, M E; Denton, D L; Murphy, K E; Owens, A C; Birnbaum, E R; John, K D; Nortier, F M; Stracener, D W; Heilbronn, L H; Mausner, L F; Mirzadeh, S

    2016-12-01

    Actinium-225 and (213)Bi have been used successfully in targeted alpha therapy (TAT) in preclinical and clinical research. This paper is a continuation of research activities aiming to expand the availability of (225)Ac. The high-energy proton spallation reaction on natural thorium metal targets has been utilized to produce millicurie quantities of (225)Ac. The results of sixteen irradiation experiments of thorium metal at beam energies between 78 and 192MeV are summarized in this work. Irradiations have been conducted at Brookhaven National Laboratory (BNL) and Los Alamos National Laboratory (LANL), while target dissolution and processing was carried out at Oak Ridge National Laboratory (ORNL). Excitation functions for actinium and thorium isotopes, as well as for some of the fission products, are presented. The cross sections for production of (225)Ac range from 3.6 to 16.7mb in the incident proton energy range of 78-192MeV. Based on these data, production of curie quantities of (225)Ac is possible by irradiating a 5.0gcm(-2 232)Th target for 10 days in either BNL or LANL proton irradiation facilities. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. 76 FR 74585 - Railroad Workplace Safety; Adjacent-Track On-Track Safety for Roadway Workers

    Science.gov (United States)

    2011-11-30

    ... fouling a track, and flagmen and watchmen/ lookouts * * *.'' See Sec. 214.7, ``Roadway worker.'' \\4\\ The... another common method of establishing on- track safety in which a trained and qualified watchman/lookout... location of the roadway worker. See Sec. Sec. 214.329 and 214.7 ``Watchman/ ] lookout.'' Train approach...

  12. Semi-blind channel estimation for MIMO-OFDM systems with VC Computer Engi-neering and Applications, 2014, 50(18):210-214%基于VC的MIMO-OFDM系统的子空间半盲信道估计

    Institute of Scientific and Technical Information of China (English)

    刘玉珍; 闫兴玉

    2014-01-01

    The convergence speed of traditional subspace-based blind channel estimation is slow and has a good estimating quality only by receiving a large number of signals. Therefore, this paper proposes a VC-based blind channel estimation method in MIMO-OFDM systems. The algorithm is the combination of traditional subspace algorithm and block matrix thought. It extracts a set of vectors to reduce the number of dimensions in OFDM signals and then adds pilot sequence to form a new semi-blind channel estimation method in blind channel estimation method.By computer modeling and simulation, it can be found that newly promoted blind channel estimation method and semi-blind channel estimation method work better in channel estimation and convergence.%传统的子空间盲信道估计收敛速度缓慢且需要大量接收信号才能保证估计性能良好,就此问题提出了一种新的基于虚拟载波(VC)的MIMO-OFDM系统的盲信道估计算法。该算法是传统子空间算法与块矩阵思想的结合,在每个OFDM符号中提取一组子矢量来降低维度。而后又在盲信道估计算法中加入导频序列形成新的半盲信道估计算法。通过计算机模拟仿真发现,新提出的盲信道和半盲信道估计算法在信道估计性能和收敛性方面均表现较好。

  13. Dietary exposure to benzoates (E210-E213), parabens (E214-E219), nitrites (E249-E250), nitrates (E251-E252), BHA (E320), BHT (E321) and aspartame (E951) in children less than 3 years old in France.

    Science.gov (United States)

    Mancini, F R; Paul, D; Gauvreau, J; Volatier, J L; Vin, K; Hulin, M

    2015-01-01

    This study aimed to estimate the exposure to seven additives (benzoates, parabens, nitrites, nitrates, BHA, BHT and aspartame) in children aged less than 3 years old in France. A conservative approach, combining individual consumption data with maximum permitted levels, was carried out for all the additives. More refined estimates using occurrence data obtained from products' labels (collected by the French Observatory of Food Quality) were conducted for those additives that exceeded the acceptable daily intake (ADI). Information on additives' occurrence was obtained from the food labels. When the ADI was still exceeded, the exposure estimate was further refined using measured concentration data, if available. When using the maximum permitted level (MPL), the ADI was exceeded for benzoates (1.94 mg kg(-1) bw day(-1)), nitrites (0.09 mg kg(-1) bw day(-1)) and BHA (0.39 mg kg(-1) bw day(-1)) in 25%, 54% and 20% of the entire study population respectively. The main food contributors identified with this approach were current foods as these additives are not authorised in specific infant food: vegetable soups and broths for both benzoates and BHA, delicatessen and meat for nitrites. The exposure estimate was significantly reduced when using occurrence data, but in the upper-bound scenario the ADI was still exceeded significantly by the age group 13-36 months for benzoates (2%) and BHA (1%), and by the age group 7-12 months (16%) and 13-36 months (58%) for nitrites. Measured concentration data were available exclusively for nitrites and the results obtained using these data showed that the nitrites' intake was below the ADI for all the population considered in this study. These results suggest that refinement of exposure, based on the assessment of food levels, is needed to estimate the exposure of children to BHA and benzoates for which the risk of exceeding the ADI cannot be excluded when using occurrence data.

  14. Cai Hua, L’homme pensé par l’homme, Du statut scientifique des sciences sociales (Man thought by Man. About the scientific status of social sciences), Paris, PUF, 2008, 214 pp. et Laurent Barry, La parenté (Kinship), Paris, Gallimard, 2008, 567 pp.

    OpenAIRE

    2012-01-01

    Two recent works reintroduce China into the anthropology of kinship. Cai Hua and Laurent Barry are both students of Françoise Héritier, who at the Collège de France has revitalised our understanding of the systems of African kinship. Their two books, similarly constructed, devote considerable space to the systems of the Han. The anthropology of kinship, a discipline founded in 1870 by Lewis Henry Morgan’s Systems of Consanguinity and Affinity of the Human Family, and revived in 1949 by the th...

  15. Superatomic S(2) silver clusters stabilized by a thiolate-phosphine monolayer: insight into electronic and optical properties of Ag14(SC6H3F2)12(PPh3)8 and Ag16(SC6H3F2)14(DPPE)4.

    Science.gov (United States)

    Gell, Lars; Lehtovaara, Lauri; Häkkinen, Hannu

    2014-09-18

    The electronic structure of two recently crystallographically solved, thiolate-phosphine protected silver clusters Ag14 and Ag16 are analyzed via density functional theory (DFT) and their optical excitations are analyzed from time-dependent DFT perturbation theory. Both clusters can be characterized as having the S(2) free-electron configuration in the metal core, which is the first time such a configuration is confirmed for structurally known ligand-protected noble metal clusters. However, their different core shapes and ligand layer induce significantly different optical spectra. Performance of gradient-corrected DFT functionals is discussed and it is shown that the asymptotically correct Leeuwen-Baerends LB94 functional reproduces the optical spectrum of Ag14 in a good agreement with experiment. Choice of the functional becomes important for clusters where the optical transitions are dominated by the electron-rich ligand layer.

  16. Process for radioisotope recovery and system for implementing same

    Science.gov (United States)

    Meikrantz, David H.; Todd, Terry A.; Tranter, Troy J.; Horwitz, E. Philip

    2007-01-02

    A method of recovering daughter isotopes from a radioisotope mixture. The method comprises providing a radioisotope mixture solution comprising at least one parent isotope. The at least one parent isotope is extracted into an organic phase, which comprises an extractant and a solvent. The organic phase is substantially continuously contacted with an aqueous phase to extract at least one daughter isotope into the aqueous phase. The aqueous phase is separated from the organic phase, such as by using an annular centrifugal contactor. The at least one daughter isotope is purified from the aqueous phase, such as by ion exchange chromatography or extraction chromatography. The at least one daughter isotope may include actinium-225, radium-225, bismuth-213, or mixtures thereof. A liquid-liquid extraction system for recovering at least one daughter isotope from a source material is also disclosed.

  17. Temporal Variations in Surface Concentrations of Terrestrial Lead 210 and Uranium 235 Radionucleides

    Science.gov (United States)

    Yoshimori, M.

    We have started to measure the surface concentrations of terrestrial radionucleides of lead 210 and uranium 235 in Tokyo (N35, E139) in 2002. Lead 210 which is a daughter nucleus of Rn-222 (uranium series) emits 46 keV gamma-ray with a half-life of 22.4 years, while uranium 235 (actinium series) emits a gamma-ray at 186 keV with a half-life of 0.7 billion years. The present measurement of the surface concentration of lead 210 exhibits two peaks in spring and fall, similar to the beryllium 7 seasonal variations, while the uranium 235 concentration does not vary with time within statistical errors. These two terrestrial radionucleides exhibit different temporal variations on the surface. We discuss possible explanations for the differences in the temporal variations from a point of view of differences in their altitude distribution.

  18. Towards high-resolution laser ionization spectroscopy of the heaviest elements in supersonic gas jet expansion

    Science.gov (United States)

    Ferrer, R.; Barzakh, A.; Bastin, B.; Beerwerth, R.; Block, M.; Creemers, P.; Grawe, H.; de Groote, R.; Delahaye, P.; Fléchard, X.; Franchoo, S.; Fritzsche, S.; Gaffney, L. P.; Ghys, L.; Gins, W.; Granados, C.; Heinke, R.; Hijazi, L.; Huyse, M.; Kron, T.; Kudryavtsev, Yu.; Laatiaoui, M.; Lecesne, N.; Loiselet, M.; Lutton, F.; Moore, I. D.; Martínez, Y.; Mogilevskiy, E.; Naubereit, P.; Piot, J.; Raeder, S.; Rothe, S.; Savajols, H.; Sels, S.; Sonnenschein, V.; Thomas, J-C; Traykov, E.; Van Beveren, C.; Van den Bergh, P.; Van Duppen, P.; Wendt, K.; Zadvornaya, A.

    2017-01-01

    Resonant laser ionization and spectroscopy are widely used techniques at radioactive ion beam facilities to produce pure beams of exotic nuclei and measure the shape, size, spin and electromagnetic multipole moments of these nuclei. However, in such measurements it is difficult to combine a high efficiency with a high spectral resolution. Here we demonstrate the on-line application of atomic laser ionization spectroscopy in a supersonic gas jet, a technique suited for high-precision studies of the ground- and isomeric-state properties of nuclei located at the extremes of stability. The technique is characterized in a measurement on actinium isotopes around the N=126 neutron shell closure. A significant improvement in the spectral resolution by more than one order of magnitude is achieved in these experiments without loss in efficiency. PMID:28224987

  19. Origin of elements of the Uranium-235 family observed in the Ellez river near the EL-4 experimental nuclear reactor in dismantling (Monts d'Arree- Finistere department); Origine des elements de la famille de l'uranium-235 observes dans la riviere Ellez a proximite du reacteur nucleaire experimental EL4 en cours de demantelement (Mont d'Arree - departement du Finistere). Resultats et premiers constats annee 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    In a previous study which concerned the catchment basin of the harbour of Brest, the A.C.R.O. put in evidence a marking by artificial radioelements around the power plant of Brennilis which can be imputed without ambiguities to the nuclear installation. It also put in evidence abnormalities concerning the natural radioactivity which justifies this new study. In the area of the Monts d'Arree, actinium 227 ({sup 227}Ac), non born by its ascendents which are {sup 235}U and {sup 231}Pa is observed. This phenomenon is characterized by mass activities superior to these ones of {sup 235}U and able to reach these ones of {sup 238}U. Its presence corresponds with the drainage of the Ellez river since the former channel of radioactive effluents releases from the nuclear power plant EL-4 up to the reservoir Saint-Herblot situated 6 km downstream. The strongest values of radioactivity are registered near the disused power plant, at this place a relationship exists between the level of actinium 227 and this one of the artificial radioactivity as it exists a relationship with the decay products of radon exhaled from the subsoil ({sup 210}Pb). But its presence is not limited to a part of the Ellez river, it is equally observed in terrestrial medium, in places in priori not influenced by the direct liquid effluents of the power plant. This place is situated at more than 4 km and without any connection with the Ellez waters. At this stage of the study, it is not possible to answer with certainty the question of the origin of this phenomenon. A new reorientation is considered indispensable to clarify definitively the origin of this unknown phenomenon in the scientific publications and the environmental monitoring. (N.C.)

  20. Evaluation of total effective dose due to certain environmentally placed naturally occurring radioactive materials using a procedural adaptation of RESRAD code.

    Science.gov (United States)

    Beauvais, Z S; Thompson, K H; Kearfott, K J

    2009-07-01

    Due to a recent upward trend in the price of uranium and subsequent increased interest in uranium mining, accurate modeling of baseline dose from environmental sources of radioactivity is of increasing interest. Residual radioactivity model and code (RESRAD) is a program used to model environmental movement and calculate the dose due to the inhalation, ingestion, and exposure to radioactive materials following a placement. This paper presents a novel use of RESRAD for the calculation of dose from non-enhanced, or ancient, naturally occurring radioactive material (NORM). In order to use RESRAD to calculate the total effective dose (TED) due to ancient NORM, a procedural adaptation was developed to negate the effects of time progressive distribution of radioactive materials. A dose due to United States' average concentrations of uranium, actinium, and thorium series radionuclides was then calculated. For adults exposed in a residential setting and assumed to eat significant amounts of food grown in NORM concentrated areas, the annual dose due to national average NORM concentrations was 0.935 mSv y(-1). A set of environmental dose factors were calculated for simple estimation of dose from uranium, thorium, and actinium series radionuclides for various age groups and exposure scenarios as a function of elemental uranium and thorium activity concentrations in groundwater and soil. The values of these factors for uranium were lowest for an adult exposed in an industrial setting: 0.00476 microSv kg Bq(-1) y(-1) for soil and 0.00596 microSv m(3) Bq(-1) y(-1) for water (assuming a 1:1 234U:238U activity ratio in water). The uranium factors were highest for infants exposed in a residential setting and assumed to ingest food grown onsite: 34.8 microSv kg Bq(-1) y(-1) in soil and 13.0 microSv m(3) Bq(-1) y(-1) in water.

  1. 两种稀土产品中放射性核素分析研究%ANALYSIS OF RADIONUCLIDES IN TWO RARE EARTH PRODUCTS

    Institute of Scientific and Technical Information of China (English)

    周程; 孙恋君

    2009-01-01

    利用高纯锗γ谱仪对两个稀土产品氯化镧和氯化钇的样品进行能谱分析,研究经湿法冶炼工艺后的稀土产品中放射性核素的活度变化.结果表明,样品中存在天然放射性钍系和锕-铀系的核素,以及天然放射性核素~(138)La和~(176)Lu,但两个样品中所含的放射性核素不相同.氯化镧产品中锕-铀系和钍系核素放射性未达平衡,其主要子体~(224)Ra和~(227)Th的含量随时间发生明显变化,约60天达到平衡;而氯化钇产品没有这种现象.%This paper describes a study on raidoactivity change of rare-earth products after hydrometallurgy technology,using HPGe gamma spectrometer to analyze two rare earth products (LaCl_3 and YCl_3).The results show that in the two samples exist natural radionuclides of thorium series and actinium-uranium series as well as other natural radionuclides 138 La and 176 Lu.In the sample of lanthanum chloride the radioactivity of actinium-uranium series and thorium series has not reached balance,the contents of their main progenies ~(226)Ra and ~(227)Th obviously changes with the time,reaching balance 60 days later,while YCl_3 keeps stability.

  2. Identification of Small Molecule Inhibitors of microRNA Involved in Chemoresistance and Cancer Stem Cells for Ovarian Cancer Intervention

    Science.gov (United States)

    2012-03-01

    d #2 0 miR-214 Fig. 6. miR-214 binds to compounds #2 and #17 in vitro. The indicated com pounds, which contain amino group (5), were immobilized ...Tang, H. (2011) Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion ofHeLa cells. Int. J. Biochem. Cell Biol. 43

  3. Interlibrary Loan Rates for Academic Libraries in the United States of America Have Increased Despite the Availability of Electronic Databases, but Fulfilment Rates Have Decreased. A Review of: Williams, J. A., & Woolwine, D. E. (2011. Interlibrary loan in the United States: An analysis of academic libraries in a digital age. Journal of Interlibrary Loan, Document Delivery & Electronic Reserve, 21(4, 165-183. doi: 10.1080/1072303X.2011.602945

    Directory of Open Access Journals (Sweden)

    Kathryn Oxborrow

    2012-12-01

    Full Text Available Objectives – To determine the number of interlibrary loan (ILL requests in academiclibraries in the United States of America over the period 1997-2008, and how various factors have influenced these rates. These factors included electronic database subscriptions,size of print journal and monograph collections, and the presence of link resolvers.Data were collected from libraries as both lenders and borrowers. The study also lookedat whether the number of professional staff in an ILL department had changed during theperiod studied, and whether ILL departments led by a professional librarian correlated positively with rates of ILL.Design – Online questionnaire.Setting – Academic library members of the Online Computer Library Center (OCLC ILL scheme in the United States of America.Subjects – A total of 442 academic library members of the OCLC ILL scheme.Methods – An electronic questionnaire was sent to 1433 academic library memberinstitutions of the OCLC ILL scheme. Datawere collected for libraries as both lending andborrowing institutions. Data were analyzed using a statistical software package,specifically to calculate Spearman’s rank correlations between the variables and rates of ILL.Main Results – Responses to the electronic questionnaire were received from 442 (31% academic libraries. There was an overall increase in the number of ILL requests in the period 1997-2008. The number of ILL requests which were unfulfilled also increased during this period. There was a positive correlation between rates of ILL and all of the variables investigated, with the strongest correlations with size of print monograph collections and size of print journal collections. The numbers of staff in ILL departments remained relatively static during the period covered by the study, although the majority of staff working in ILL was composed of paraprofessionals. There was a weak positive correlation between numbers of ILL requests and whether ILL departments were headed by a professional librarian.Conclusions – Access to full text electronic databases has not decreased the numbers of ILL requests in academic libraries in the United States of America. In fact, ILL requests have increased, probably due to the fact that students and staff of academic libraries now have access to a larger number of citations through online databases and other information sources. The authors suggest that the increase in unfulfilled ILL requests is also due to this increased access. Libraries with large print collections are more likely to receive ILL requests precisely because they have more material to lend out, and may make more ILL requests due to the research output of their presumably larger institutions. There may be a higher number of ILL requests fulfilled by departments headed by a professional librarian because a librarian has more knowledge of sources to fulfil requests.

  4. Fracturas supracondíleas del húmero en niños entre 2 y 14 años. Perfil demográfico y de tratamiento en el Hospital Pablo Tobón Uribe, Medellín, Colombia = Supracondylar fractures of the humerus in children aged 2-14 years. Demographic profile and therapeutic approach at a third-level hospital in Medellín, Colombia

    Directory of Open Access Journals (Sweden)

    Valderrama Molina, Carlos Oliver

    2011-12-01

    Full Text Available Introducción: las fracturas supracondíleas del húmero distal son las lesiones más frecuentes de tratamiento quirúrgico en la población pediátrica. Los informes sobre el perfil demográfico y el enfoque terapéutico de las mismas en Medellín son limitados; este trabajo se llevó a cabo para conocer la epidemiología local de estas lesiones y detectar en el seguimiento los aciertos y errores del tratamiento como base para desarrollar estudios de mayor complejidad.Materiales y métodos: mediante una búsqueda exhaustiva en los registros de historias clíni­cas electrónicas se encontraron 205 casos de fracturas supracondíleas del húmero en niños entre dos y 14 años que ingresaron al Servicio de Urgencias del Hospital Pablo Tobón Uribe entre enero de 2005 y septiembre de 2009. Se analizaron en ellas los aspectos demográficos y terapéuticos.Resultados: el 61,5% de los pacientes fueron varones, la media de la edad fue de 6,6 años, en 62,4% de los casos la fractura afectó el lado izquierdo. La clasificación (Gartland fue como sigue: tipo I: 41,5%; tipo II: 12,2%; tipo III: 46,3%. El 95,6% fueron fracturas cerradas. Se hizo tratamiento quirúrgico en 103 casos (50,2%. El tiempo promedio entre el ingreso y la cirugía fue de 6,7 horas. La técnica quirúrgica más frecuente fue la reducción cerrada con fijación percutánea con clavos cruzados (45,6% de los casos. Se observaron 31 casos (15,1% de le­siones neurológicas, 28 de ellas del nervio ulnar; nueve de las 31 lesiones se registraron en la evaluación posquirúrgica inmediata, todas ellas en pacientes tratados con clavos cruzados. No se registraron lesiones vasculares ni síndromes compartimentales.Conclusión: esta serie presenta el estado actual del perfil demográfico de los pacientes con fracturas supracondíleas del húmero distal en niños y de las modalidades de tratamiento en un hospital de tercer nivel de la ciudad de Medellín. Los resultados pueden ser la base para futuros estudios de mayor elaboración metodológica.

  5. Cumulative Reactant Species Index for Volumes I-V of the Compilation of Data Relevant to Gas Lasers. Volume VI.

    Science.gov (United States)

    1979-09-01

    cross section (relative) 2027 ICt Electron affinity 214, 1161 Extinction coefficient for continuous absorption 702, 2032 12 Electron affinity 214, 1161...864 On Ne, high-energy production of free electrons 1622 On W, elt -ctron yields from surface 865 On Xe, high-energy ionization 1624 On He, high-energy

  6. NCBI nr-aa BLAST: CBRC-XTRO-01-0526 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0526 ref|YP_981084.1| protein of unknown function DUF214 [Polaromonas naphtha...lenivorans CJ2] gb|ABM36163.1| protein of unknown function DUF214 [Polaromonas naphthalenivorans CJ2] YP_981084.1 0.0 65% ...

  7. AcEST: DK962281 [AcEST

    Lifescience Database Archive (English)

    Full Text Available OS=Glycine max ... 214 2e-55 sp|Q9LIA8|UGDH1_ARATH Probable UDP-glucose 6-dehydrogenase 1 OS=... 214 2e-55 s...FKTLDYQKIFDNMQKPAFVFDGRNIVDADKLREIGFIVYSIGKPLDPWLKDMPA 478 >sp|Q9LIA8|UGDH1_ARATH Probable UDP-glucose 6-deh

  8. 49 CFR 585.75 - Response to inquiries.

    Science.gov (United States)

    2010-10-01

    ... moving deformable barrier test with advanced test dummies (S7.2) or the vehicles (by make, model and... requirements (S9.1) of FMVSS No. 214 (49 CFR 571.214). The manufacturer's designation of a vehicle as a certified vehicle that meets S7.2 or S9.1 is irrevocable....

  9. Eight-Year Summary of Fort Irwin, CA, Family Housing Comparison Test: Operation and Maintenance Costs of Manufactured vs. Conventionally Built Units

    Science.gov (United States)

    1993-07-01

    and mantaining the data needed, and complelng and revneeng the conatiedon of infoernian. Send comments regaiding this burden asmae or any other aspec...75039 Mexico Toll-Free Number. 800-526-4960 222 West Las Colinas Blvd., Suite 2030 Colanoso Mexicana S.A. 214-556-2585/Fax: 214-556-2589 Mexico 20, D.F

  10. On a Semi-Coercive Quasi-Variational Inequality.

    Science.gov (United States)

    1980-06-01

    Roma, Italy. 2Departement de Mathematique , C.P.214, Universite Libre de Bruxelles, 1050 Bruxl~e BA~q~um, sponsored by the United States Army under...di Roma, 00100 Roma, Italy. 2 Departement de Mathematique , C.P.214, Universite Libre de Bruxelles, 1050 Bruxelles, Belgium, sponsored by the United

  11. NCBI nr-aa BLAST: CBRC-DRER-17-0045 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DRER-17-0045 ref|YP_921174.1| protein of unknown function DUF214 [Thermofilum ...pendens Hrk 5] gb|ABL79171.1| protein of unknown function DUF214 [Thermofilum pendens Hrk 5] YP_921174.1 2.1 32% ...

  12. Excess molar volumes of (an alkanol plus a branched chain ether) at the temperature 298.15 K and the application of the ERAS model

    CSIR Research Space (South Africa)

    Letcher, TM

    1997-12-01

    Full Text Available . 216 TM. Letcher, P.U. Gol;ender/ Fluid Phase Equilibria 140 (1997) 207-220 x Fig. 2 (see p. 214 for caption). T.M. Letcher, P. I/. Gownder/ Fluid Phase Equilibricr 140 C I9971 207-220 (4 Fig. 3 (see p. 214 for caption...

  13. NCBI nr-aa BLAST: CBRC-TSYR-01-1170 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TSYR-01-1170 ref|YP_001989230.1| protein of unknown function DUF214 [Rhodopseudomonas... palustris TIE-1] gb|ACE98754.1| protein of unknown function DUF214 [Rhodopseudomonas palustris TIE-1] YP_001989230.1 0.021 27% ...

  14. NCBI nr-aa BLAST: CBRC-XTRO-01-0526 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0526 ref|YP_972143.1| protein of unknown function DUF214 [Acidovorax a...venae subsp. citrulli AAC00-1] gb|ABM34369.1| protein of unknown function DUF214 [Acidovorax avenae subsp. citrulli AAC00-1] YP_972143.1 0.0 74% ...

  15. NCBI nr-aa BLAST: CBRC-XTRO-01-0526 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0526 ref|YP_987648.1| protein of unknown function DUF214 [Acidovorax s...p. JS42] gb|ABM43572.1| protein of unknown function DUF214 [Acidovorax sp. JS42] YP_987648.1 0.0 74% ...

  16. Combined Transmission of Baseband NRZ-DQPSK and Phase Modulated Radio-over-Fibre

    DEFF Research Database (Denmark)

    Jensen, Jesper Bevensee; Yu, Xianbin; Tafur Monroy, Idelfonso

    2008-01-01

    21.4 Gbit/s baseband DQPSK and 1.25 Gbit/s phase modulated RoF was transmitted over 80 km SSMF using polarization multiplexing.......21.4 Gbit/s baseband DQPSK and 1.25 Gbit/s phase modulated RoF was transmitted over 80 km SSMF using polarization multiplexing....

  17. Characterization of polar organosulfates in secondary organic aerosol from the green leaf volatile 3-Z-hexenal

    Science.gov (United States)

    Evidence is provided that the green leaf volatile 3-Z-hexenal serves as a precursor for biogenic secondary organic aerosol through formation of polar organosulfates (OSs) with molecular weights (MW) 226 and 214. The MW 226 C6-OSs and MW 214 C5M-OSs were che...

  18. 77 FR 65546 - Western Area Power Administration; Notice of Filing

    Science.gov (United States)

    2012-10-29

    ... for the Western Rate Schedules database, to be effective July 14, 2011. Any person desiring to intervene or to protest this filing must file in accordance with Rules 211 and 214 of the Commission's Rules of Practice and Procedure (18 CFR 385.211, 385.214). Protests will be considered by the Commission...

  19. Integrated inventory information system

    Digital Repository Service at National Institute of Oceanography (India)

    Sarupria, J.S.; Kunte, P.D.

    stream_size 5 stream_content_type text/plain stream_name Handbook_Lib_Archives_Inf_Cen_India_7_214.pdf.txt stream_source_info Handbook_Lib_Archives_Inf_Cen_India_7_214.pdf.txt Content-Encoding ISO-8859-1 Content-Type text...

  20. NCBI nr-aa BLAST: CBRC-XTRO-01-0526 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0526 ref|YP_547798.1| protein of unknown function DUF214 [Polaromonas ...sp. JS666] gb|ABE42900.1| protein of unknown function DUF214 [Polaromonas sp. JS666] YP_547798.1 0.0 66% ...

  1. 75 FR 67059 - Public Hearings for Greenhouse Gas Emissions Standards and Fuel Efficiency Standards for Medium...

    Science.gov (United States)

    2010-11-01

    ... INFORMATION CONTACT: If you would like to present oral testimony at a public hearing, please contact Julia Mac... 48105; telephone number: (734) 214-4131; fax number: (734) 214-4050; e-mail address: macallister.julia... numbers, and whether you require accommodations such as a sign language interpreter. Questions...

  2. 77 FR 63848 - Report on Carcinogens Evaluation of Pentachlorophenol; Request for Nominations of Scientific Experts

    Science.gov (United States)

    2012-10-17

    ... cancer risk and invites the nomination of speakers. Nominees should have expertise in cancer epidemiology.... Ruth Lunn, Director, ORoC, DNTP, NIEHS, P.O. Box 12233, MD K2-14, Research Triangle Park, NC 27709..., NIEHS, P.O. Box 12233, MD K2-14, Research Triangle Park, NC 27709; telephone (919) 316-4637; FAX:...

  3. NCBI nr-aa BLAST: CBRC-MEUG-01-1943 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-1943 ref|YP_002018491.1| protein of unknown function DUF214 [Pelodictyon phaeocl...athratiforme BU-1] gb|ACF43874.1| protein of unknown function DUF214 [Pelodictyon phaeoclathratiforme BU-1] YP_002018491.1 8.3 35% ...

  4. NCBI nr-aa BLAST: CBRC-XTRO-01-0526 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0526 ref|ZP_01517730.1| protein of unknown function DUF214 [Comamonas ...testosteroni KF-1] gb|EAV17199.1| protein of unknown function DUF214 [Comamonas testosteroni KF-1] ZP_01517730.1 0.0 70% ...

  5. Comparison of Activity Determination of Radium 226 in FUSRAP Soil using Various Energy Lines - 12299

    Energy Technology Data Exchange (ETDEWEB)

    Tucker, Brian [Shaw Environmental and Infrastructure, Stoughton, MA 02072 (United States); Donakowski, Jough [Unites States Army Corps of Engineers, Kansas City, MO 64106 (United States); Hays, David [United States Army Corps of Engineers, Maywood, NJ 07607 (United States)

    2012-07-01

    Gamma spectroscopy is used at the Formerly Utilized Sites Remedial Action Program (FUSRAP) Maywood Superfund Site as the primary radioanalytical tool for quantization of activities of the radionuclides of concern in site soil. When selecting energy lines in gamma spectroscopy, a number of factors are considered including assumptions concerning secondary equilibrium, interferences, and the strength of the lines. The case of the Maywood radionuclide of concern radium-226 (Ra-226) is considered in this paper. At the FUSRAP Maywood Superfund Site, one of the daughters produced from radioactive decay of Ra-226, lead-214 (Pb- 214), is used to quantitate Ra-226. Another Ra-226 daughter, bismuth-214 (Bi-214), also may be used to quantitate Ra-226. In this paper, a comparison of Ra-226 to Pb-214 activities and Ra-226 to Bi-214 activities, obtained using gamma spectrometry for a large number of soil samples, was performed. The Pb-214, Bi-214, and Ra-226 activities were quantitated using the 352 kilo electron volt (keV), 609 keV, and 186 keV lines, respectively. The comparisons were made after correcting the Ra-226 activities by a factor of 0.571 and both ignoring and accounting for the contribution of a U-235 interfering line to the Ra-226 line. For the Pb-214 and Bi-214 activities, a mean in-growth factor was employed. The gamma spectrometer was calibrated for efficiency and energy using a mixed gamma standard and an energy range of 59 keV to 1830 keV. The authors expect other sites with Ra-226 contamination in soil may benefit from the discussions and points in this paper. Proper use of correction factors and comparison of the data from three different gamma-emitting radionuclides revealed agreement with expectations and provided confidence that using such correction factors generates quality data. The results indicate that if contamination is low level and due to NORM, the Ra-226 can be measured directly if corrected to subtract the contribution from U-235. If there is

  6. TRASH TO TREASURE: CONVERTING COLD WAR LEGACY WASTE INTO WEAPONS AGAINST CANCER

    Energy Technology Data Exchange (ETDEWEB)

    Nicholas, R.G.; Lacy, N.H.; Butz, T.R.; Brandon, N.E.

    2004-10-06

    As part of its commitment to clean up Cold War legacy sites, the U.S. Department of Energy (DOE) has initiated an exciting and unique project to dispose of its inventory of uranium-233 (233U) stored at Oak Ridge National Laboratory (ORNL), and extract isotopes that show great promise in the treatment of deadly cancers. In addition to increasing the supply of potentially useful medical isotopes, the project will rid DOE of a nuclear concern and cut surveillance and security costs. For more than 30 years, DOE's ORNL has stored over 1,200 containers of fissile 233U, originally produced for several defense-related projects, including a pilot study that looked at using 233U as a commercial reactor fuel. This uranium, designated as special nuclear material, requires expensive security, safety, and environmental controls. It has been stored at an ORNL facility, Building 3019A, that dates back to the Manhattan Project. Down-blending the material to a safer form, rather than continuing to store it, will eliminate a $15 million a year financial liability for the DOE and increase the supply of medical isotopes by 5,700 percent. During the down-blending process, thorium-229 (229Th) will be extracted. The thorium will then be used to extract actinium-225 (225Ac), which will ultimately supply its progeny, bismuth-213 (213Bi), for on-going cancer research. The research includes Phase II clinical trials for the treatment of acute myelogenous leukemia at Sloan-Kettering Memorial Cancer Center in New York, as well as other serious cancers of the lungs, pancreas, and kidneys using a technique known as alpha-particle radioimmunotherapy. Alpha-particle radioimmunotherapy is based on the emission of alpha particles by radionuclides. 213Bi is attached to a monoclonal antibody that targets specific cells. The bismuth then delivers a high-powered but short-range radiation dose, effectively killing the cancerous cells but sparing the surrounding tissue. Production of the actinium and

  7. Dicty_cDB: SHJ885 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available / 765 0.0 SHD214 (SHD214Q) /CSM/SH/SHD2-A/SHD214Q.Seq.d/ 765 0.0 SHD213 (SHD213Q) /CSM/SH/SHD2-A/SHD213Q.Seq.d/ 765 0.0 SHA293 (SHA29...3Q) /CSM/SH/SHA2-D/SHA293Q.Seq.d/ 765 0.0 CHS117 (CHS117Q) /CSM/CH/CHS1-A/CHS117Q.S

  8. Dicty_cDB: SHG652 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 67 0.0 SHD214 (SHD214Q) /CSM/SH/SHD2-A/SHD214Q.Seq.d/ 767 0.0 SHD213 (SHD213Q) /CSM/SH/SHD2-A/SHD213Q.Seq.d/ 767 0.0 SHA293 (SHA29...3Q) /CSM/SH/SHA2-D/SHA293Q.Seq.d/ 767 0.0 CHS117 (CHS117Q) /CSM/CH/CHS1-A/CHS117Q.Seq.

  9. Dicty_cDB: SHJ637 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available eq.d/ 767 0.0 SHD214 (SHD214Q) /CSM/SH/SHD2-A/SHD214Q.Seq.d/ 767 0.0 SHD213 (SHD213Q) /CSM/SH/SHD2-A/SHD213Q.Seq.d/ 767 0.0 SHA29...3 (SHA293Q) /CSM/SH/SHA2-D/SHA293Q.Seq.d/ 767 0.0 CHS117 (CHS117Q) /CSM/CH/CHS1-A/CHS11

  10. Dicty_cDB: SHF893 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2Q.Seq.d/ 706 0.0 SHD214 (SHD214Q) /CSM/SH/SHD2-A/SHD214Q.Seq.d/ 706 0.0 SHD213 (SHD213Q) /CSM/SH/SHD2-A/SHD213Q.Seq.d/ 706 0.0 SHA29...3 (SHA293Q) /CSM/SH/SHA2-D/SHA293Q.Seq.d/ 706 0.0 CHS117 (CHS117Q) /CSM/CH/CHS1-A/C

  11. Dicty_cDB: CHN559 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .0 SHD214 (SHD214Q) /CSM/SH/SHD2-A/SHD214Q.Seq.d/ 747 0.0 SHD213 (SHD213Q) /CSM/SH/SHD2-A/SHD213Q.Seq.d/ 747 0.0 SHA293 (SHA29...3Q) /CSM/SH/SHA2-D/SHA293Q.Seq.d/ 747 0.0 CHS117 (CHS117Q) /CSM/CH/CHS1-A/CHS117Q.Seq.d/ 7

  12. 基于STEP的特征模型及重构算法%STEP-Based Feature Model and Feature Reconstructed Arithmetic

    Institute of Scientific and Technical Information of China (English)

    刘乃若; 王金伦

    2003-01-01

    For CAX systems,the technology of Feature-based product data integration is one of hot points. STEP AP214 protocol provides a standard to resolve this problem. This paper discusses the relations of entities in STEP AP214. Especially,for the problem that the protocol doesn't obviously give those features,it puts forward methods on expression and operation of feature-oriented data model. It gives the feature model mapping between AP214 and feature-based CAD systems,which is a basal theory to design out a uniform feature model of CAD/CAPP/CAM.

  13. Study on Chemical Components of Alkaline Nitrobenzene Oxidation from Plantation Woods

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    This paper deals with the chemical components and the chemical structure of lignin of Chinese fir and Poplar I-214 from plantationforest. The results revealed that the contents ofchemical component in heart wood and sapwood were almost the same except ethanol-benzene extract both in Chinese fir and in Poplar I-214, The contents of ash, holocellulose, α -cellulose, pentosan, ethanol-benzene extract and cold-water extract in Poplar I-214 were higher than that in Chinesefir, The content of lignin in Poplar...

  14. A wavelength tunable photon source with sealed inner volume

    DEFF Research Database (Denmark)

    2014-01-01

    There is presented a method of providing a wavelength tunable photon source (200), comprising bonding a first element (101) with a first mirror (106), a second element (102) with a second mirror (108) and a third element (103) with a photon emitter together in a structure enclosing an inner volume...... (214) being a sealed volume, and forming a bonding interface (212) which is gas-tight, so that the first mirror (106) is placed in the inner volume (214) so the first mirror (106) may move within the inner volume (214). The method provides a relatively simple way of obtaining a tunable photon source...

  15. About Military Sexual Trauma

    Medline Plus

    Full Text Available ... navigation Sign in Search Loading... Close Yeah, keep it Undo Close This video is unavailable. Watch Queue ... 1,801 views 2:14 She Wore These: It's our job to give her the best care ...

  16. ORIGINAL ARTICLES

    African Journals Online (AJOL)

    (197/214) mentioned mosquito bites as the cause of malaria. The respondents' ..... evidenced by the different types of dwellings. Community participation in health ... and donation of blood for active case detection. This vertical approach to ...

  17. Teater

    Index Scriptorium Estoniae

    2003-01-01

    KaTeater osaleb 2.-14. dets. A. Alegria draamaga "Niagaara" (lav. J. Urvet) Venetsueelas, Caracases VI rahvusvahelisel teatrifestivalil "Fiesta 2003". Eesti Teatriliidu volikogu koosolekul 17. nov. kinnitati R. Oja ettepanekul T. Tuisk ja A. Lumiste juhatuse uuteks liikmeteks

  18. Disease: H01139 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available crobiol Infect Dis 72:214-8 (2012) PMID:17114714 Bakken JS, Dumler JS Clinical diagnosis and treatment of human granulocytotropic anaplasmosis. Ann N Y Acad Sci 1078:236-47 (2006) ...

  19. Web-based research publications on Sub-Saharan Africa's prized ...

    African Journals Online (AJOL)

    The paper assessed articles on grasscutter in journals present online. ... Physiology (22.86%) was the most researched area while Economics and Marketing were the least (2.14%). ... Keywords: Grasscutter, research articles, online, global ...

  20. Teater

    Index Scriptorium Estoniae

    2003-01-01

    KaTeater osaleb 2.-14. dets. A. Alegria draamaga "Niagaara" (lav. J. Urvet) Venetsueelas, Caracases VI rahvusvahelisel teatrifestivalil "Fiesta 2003". Eesti Teatriliidu volikogu koosolekul 17. nov. kinnitati R. Oja ettepanekul T. Tuisk ja A. Lumiste juhatuse uuteks liikmeteks

  1. Moskvas algab tagaselja protsess Berezovski üle / Igor Taro

    Index Scriptorium Estoniae

    Taro, Igor

    2007-01-01

    Vene poliitemigranti, Suurbritanniast asüüli palunud Boriss Berezovskit süüdistatakse lennukompaniist Aeroflot 214 miljoni rubla varastamises ning rahapesus, kriminaalasja eelistung algab juulis. Lisa: Aerofloti saaga

  2. 78 FR 9902 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-02-12

    ..., LLC, Doswell Limited Partnership, Las Vegas Power Company, LLC, LS Power Marketing, LLC, LSP Safe... 385.211 and 385.214) on or before 5:00 p.m. Eastern time on the specified comment date. Protests...

  3. 78 FR 5436 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-01-25

    ... Limited Partnership. Description: Michigan Power Limited Partnership submits Supplement to 11/01/2012... Commission's Regulations (18 CFR 385.211 and 385.214) on or before 5:00 p.m. Eastern time on the...

  4. Browse Title Index

    African Journals Online (AJOL)

    Items 51 - 100 of 214 ... ... Democracy: Challenges of Promoting Democratic Education, Abstract ... in Sakhisizwe Local Municipality Area of South Africa: Obstacles and ... (OVC) in institutions and community-based settings in Zimbabwe, Abstract.

  5. Drug: D09594 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D09594 Mixture, Drug Olmesartan medoxomil - azelnidipine mixt; Rezaltas (TN) Olmesa...nts 214 Antihypertensives 2149 Others D09594 Olmesartan medoxomil - azelnidipine mixt PubChem: 124490334 ...

  6. Wave attenuation in the Agulhas current

    CSIR Research Space (South Africa)

    Grundlingh, M

    1995-07-01

    Full Text Available . J. 65, 49 - 57. 2. Taljaard LJ. (1972). Synoptic meteorology of the southern hemisphere. In Meteorology of the Southern Hemisphere, Meteorological Monographs 13, ed. W. Newton, pp. 139 - 214. American Meteorological Society, Boston. 3. Schumann...

  7. 76 FR 22686 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-04-22

    ... proceedings must file in accordance with Rules 211 and 214 of the Commission's Rules of Practice and Procedure...: Market based rate tariff database to be effective 4/ 15/2011. Filed Date: 04/14/2011. Accession...

  8. 75 FR 74707 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-12-01

    ... proceedings must file in accordance with Rules 211 and 214 of the Commission's Rules of Practice and Procedure...: Cancel Tariff Database to be effective 1/10/2011. Filed Date: 11/12/2010. Accession Number:...

  9. 75 FR 61721 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-10-06

    ... with Rules 211 and 214 of the Commission's Rules of Practice and Procedure (18 CFR 385.211 and Sec. 385.... Description: Alabama Power Company submits its Tariff Title (Tariff ] Database)--Market Based Rate...

  10. Drug: D01369 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available . HCl 381.1707 381.8936 D01369.gif Anti-anginal; Antihypertensive; Cardiac depressant [anti-arrythmic] Thera...individual organs 21 Cardiovascular agents 214 Antihypertensives 2149 Others D013

  11. Drug: D00624 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 441.6046 D00624.gif Antihypertensive Same as: C07707 Therapeutic category: 2144 ATC code: C09AA04 prodrug, ...01] 2 Agents affecting individual organs 21 Cardiovascular agents 214 Antihypertensives 2144 Angiotensin con

  12. Drug: D01302 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 516 196.6369 D01302.gif Antihypertensive Therapeutic category: 2142 ATC code: C02DB02 Genomic biomarker: NAT...ffecting individual organs 21 Cardiovascular agents 214 Antihypertensives 2142 Hy

  13. Drug: D01667 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 489.0036 D01667.gif Antihypertensive; Enzyme inhibitor [angiotensin-converting] Therapeutic category: 2144 ...ic category of drugs in Japan [BR:br08301] 2 Agents affecting individual organs 21 Cardiovascular agents 214 Antihypertensive

  14. Tabaquismo, higiene bucal y periodontopatías inmunoinflamatorias crónicas en adultos del municipio Guanajay Smoking, oral higiene and chronic immunoinflammatory periodontopathies in adults of Guanajay municipality

    National Research Council Canada - National Science Library

    Eladio Miguel Traviesas Herrera; Ricardo Rodríguez Llanes

    2007-01-01

    ... un estudio no experimental que comenzó prospectivo y continuó descriptivo; la muestra fue intencional de sujetos tipo, voluntarios, y la constituyeron 214 pacientes fumadores mayores de 19 años...

  15. 75 FR 6813 - Approval of Air Quality Implementation Plans; New Mexico; Albuquerque/Bernalillo County

    Science.gov (United States)

    2010-02-11

    ... this rulemaking should be directed to Ms. Carrie Paige, Air Planning Section (6PD-L), Environmental...; fax number 214-665- 7263; e-mail address paige.carrie@epa.gov or Mr. Bill Deese, Air Planning...

  16. 75 FR 16075 - Certain Preserved Mushrooms From the People's Republic of China: Notice of Initiation of...

    Science.gov (United States)

    2010-03-31

    ... Administration, U.S. Department of Commerce, 14th Street and Constitution Avenue, NW., Washington, DC 20230... the Act and 19 CFR 351.214(h)(i). In cases involving non-market economies, the Department...

  17. Predictors of vaginal delivery in nulliparous mothers

    African Journals Online (AJOL)

    study. Vaginal delivery was achieved in 214 (74.8%) of the eligible parturient, while 72 (25.2%) had ..... Table 2: Logistic regression of factors influencing vaginal delivery in primigravidae ... times higher risk of emergency caesarean section.

  18. 881 Institute of Ecology and Envir

    African Journals Online (AJOL)

    USER

    2015-11-02

    Nov 2, 2015 ... The potential of maize cob was established for the treatment of lead polluted wastewater. Key Words: Maize cob; Heavy metals; wastewater; Adsorption isotherms. Introduction ..... by use of natural zeolite. Desalination, 214: ...

  19. AcEST: DK960679 [AcEST

    Lifescience Database Archive (English)

    Full Text Available OS=Arabidopsis ... 145 1e-34 sp|Q94B08|GCP1_ARATH Germination-specific cysteine ...TVAAVEGINKIVTGNLTTLSEQELIDCDTTYNNGCNGGLMDYAFEYIV 214 >sp|Q94B08|GCP1_ARATH Germination-specific cysteine pro

  20. Aerospace Medicine and Biology: A cumulative index to the 1980 issues

    Science.gov (United States)

    1981-01-01

    A cumulative index to the abstracts contained in the Supplements 203 through 214 of Aerospace Medicine and Biology: A Continuing Bibliography is presented. It includes three indexes--subject, personal author, and corporate source.

  1. EST Table: FS888238 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available tances) 10/09/28 49 %/214 aa ref|XP_623145.2| PREDICTED:...e mechanism)|GO:0016020(membrane)|GO:0016820(hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of subs

  2. Scrap metals' role in circular economy in Ghana, using Sunyani as a ...

    African Journals Online (AJOL)

    EJIRO

    Department of Energy and Environmental Engineering, University of Energy and Natural Resources, P.O. Box 214,. Sunyani, Ghana. Received 27 ... Linked value chains where zero waste is generated ..... scale-up across global supply chains.

  3. Drug: D05947 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D05947 Drug Sulfacetamide (USP/INN) C8H10N2O3S 214.0412 214.2416 D05947.gif Antibacterial...ECTIVES S01AB Sulfonamides S01AB04 Sulfacetamide D05947 Sulfacetamide (USP/INN) USP drug classification [BR:br08302] Antibacterial...s Sulfonamides Sulfacetamide D05947 Sulfacetamide (USP/INN) Antiinfectives [BR:br08307] Antibacterial

  4. High conservation of a 5' element required for RNA editing of a C target in chloroplast psbE transcripts.

    Science.gov (United States)

    Hayes, Michael L; Hanson, Maureen R

    2008-09-01

    C-to-U editing modifies 30-40 distinct nucleotides within higher-plant chloroplast transcripts. Many C targets are located at the same position in homologous genes from different plants; these either could have emerged independently or could share a common origin. The 5' sequence GCCGUU, required for editing of C214 in tobacco psbE in vitro, is one of the few identified editing cis-elements. We investigated psbE sequences from many plant species to determine in what lineage(s) editing of psbE C214 emerged and whether the cis-element identified in tobacco is conserved in plants with a C214. The GCCGUU sequence is present at a high frequency in plants that carry a C214 in psbE. However, Sciadopitys verticillata (Pinophyta) edits C214 despite the presence of nucleotide differences compared to the conserved cis-element. The C214 site in psbE genes is represented in members of four branches of spermatophytes but not in gnetophytes, resulting in the parsimonious prediction that editing of psbE C214 was present in the ancestor of spermatophytes. Extracts from chloroplasts from a species that has a difference in the motif and lacks the C target are incapable of editing tobacco psbE C214 substrates, implying that the critical trans-acting protein factors were not retained without a C target. Because noncoding sequences are less constrained than coding regions, we analyzed sequences 5' to two C editing targets located within coding regions to search for possible editing-related conserved elements. Putative editing cis-elements were uncovered in the 5' UTRs near editing sites psbL C2 and ndhD C2.

  5. Radioactivity concentration measurement and analysis in construction floor materials of Korea

    Science.gov (United States)

    Kim, G. H.; Lee, H. K.; Cho, J. H.

    2016-05-01

    In this study, the radioactive concentrations contained in samples of commonly used building floor materials were measured. This result can be used as basic information for public health and the environment. Among building floor materials, samples of induction blocks, cement bricks, artificial granite blocks and compact high-pressure blocks were chosen and used. A detailed gamma nuclide analysis was performed with a multichannel analyzer by putting these samples on a high-purity germanium detector which is a semiconductor detector. In order to measure the concentration of radionuclides, a spectrum file was obtained by analyzing the concentration of gamma radionuclides and setting the measurement time as 1000, 4000, 7000 and 10,000 s. According to the study results, K-40, Bi-214, Pb-214, Ra-226 and U-235 were detected in the induction blocks measured at 10,000 s and K-40, Th-230, Bi-214, Pb-214, Ra-226 and Na-22 were detected in the cement bricks measured at 10,000 s. K-40, Bi-214, Pb-214, Th-234, U-235 and Ra-223 were detected in the artificial granite blocks measured at 10,000 s and K-40, Bi-214, Pb-214, Th-234, Ra-226, Ra-223 and Mn-54 were detected in the compact high-pressure blocks. In conclusion, low-level radioactivity was detected in building floor materials, so it is thought that measures to reduce radioactivity and further studies on this will be needed.

  6. EST Table: FS748683 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available tein analogous to peritrophins 3-B [Tribolium castaneum] gb|ABL73928.1| obstractor B [Tribolium castaneum] g...:43321197:-1|gene:AGAP009790 10/09/10 65 %/214 aa gnl|Amel|GB17664-PA 10/09/10 71 %/214 aa gi|119387886|gb|ABL73928.1| obstractor B [Tribolium castaneum] FS939122 caL- ...

  7. Functional promoter haplotypes of the human FAS gene are associated with the phenotype of SLE characterized by thrombocytopenia

    DEFF Research Database (Denmark)

    Nolsøe, R L; Kelly, J A; Pociot, F;

    2005-01-01

    to SLE or subphenotypes in 126 multiplex American SLE pedigrees and found association of the FAS codon214 AC(C/T) as well as the FAS-670G>A'-codon214 AC(C/T)' haplotype to thrombocytopenia in SLE. Furthermore we have functionally characterized the FAS/FASL promoter polymorphisms associated with SLE...... variant, determines the promoter activity. We conclude that the FAS/FASL promoter haplotypes are functional and that polymorphisms in FAS may contribute to thrombocytopenia in SLE....

  8. Federal Logistics Information System. FLIS Procedures Manual Publications. Volume 15.

    Science.gov (United States)

    1995-01-01

    4,5,7,15 dress Code DEMIL Demilitarization 4,15 DoDAAD Department of De- fense Activity Ad- DESC Defense Electronics 2,14 dress Dictionary Supply Center...Supply 2.14 DoDAAC Department of De- Center fense Activity Ad- dress Code iv CH 2 DoD 4100.39-M Volume 15 Volume(s) Volume(s) DoDAAD Department of De

  9. Drug: D09830 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D09830 Drug Fasoracetam hydrate (JAN) C10H16N2O2. H2O 214.1317 214.2615 D09830.gif ...PubChem: 124490569 LigandBox: D09830 ATOM 15 1 C1x C 22.8200 -28.4900 2 C1x C 22.8200 -29.8900 3 C1x C 24.01

  10. Evaluation of decay data of radium-226 and its daughters

    Energy Technology Data Exchange (ETDEWEB)

    Chiste, V.; Be, M.M.; Dulieu, C. [CEA Saclay, Lab. National Henri Becquerel, LNE - CEA/LNHB, 91 - Gif-sur-Yvette (France)

    2008-07-01

    A nuclear data evaluation (half-lives, emission energies and probabilities) of the nuclides of the Ra-226 chain ending at Pb-210 (not included) has been performed taking into account the new data available in the literature. The need for new absolute measurements of the decay parameters for some nuclides of the decay chain is also emphasized, the most critical cases are Pb-214 and Bi-214, for them there have not been any new measurements since 1931 and 1956 respectively.

  11. Imaging System and Method for Biomedical Analysis

    Science.gov (United States)

    2013-03-11

    fluorescent nanoparticles . Generally, Noiseux et al. teach injecting multiple fluorescent nanoparticle dyes into the food sample, imaging the sample a...example, AIDS, malaria , cholera, lymphoma, and typhoid. The present disclosure can be used to capture and count microscopic cells for application as...Base plate 214 is sealed against cover 204 by the adhesive 210. Base plate 214 can have a thickness 216 of, for example, about 100 µm. At least a

  12. Report on scientist exchange program -analysis of uranium series nuclides in rocks and minerals-

    OpenAIRE

    1992-01-01

    This report mainly described the analysis of uranium series nuclidesin samples of rocks and minerials, giving detailed procedures about them. The related analytical methods and techniques included alpha spectrometry for U-238,U-234,Th-232 and Th-230, which employedanion exchange and TBP-tetrachloromethane extraction methods separating uranium and thorium from matrix and electrode position method preparing source disks, gamma spectrometry for Pb-210, Pb-214, Bi-214, Th-234 and Ra-226, oxine-ch...

  13. Domain Modeling: NP_714542.1 [SAHG[Archive

    Lifescience Database Archive (English)

    Full Text Available NP_714542.1 chr9 Insights into the Alkyl Peroxide Reduction Activity of Xanthomonas... campestris Bacterioferritin Comigratory Protein from the Trapped Intermediate/Ligand Complex Structures p3gkka_ chr9/NP_714542....1/NP_714542.1_apo_22-214.pdb p3gkma_ chr9/NP_714542.1/NP_714542.1_holo_22-214.pdb psi-bl

  14. A primary standard source of radon-222 based on the HPGe detector.

    Science.gov (United States)

    Mostafa, M Y A; Vasyanovich, M; Zhukovsky, M

    2017-02-01

    The present paper describes the prototype of a calibration standard system for radon concentrations to be used in establishing the traceability of radon concentration measurements in dwellings. Radon gas was generated with a radium-226 solid source in a certified volume as a closed system. The activity of the radon that was released in the closed system was determined from the difference between the absolute activity of the standard radium solid source and the residual radon decay products ((214)Bi or (214)Pb). A high-purity germanium (HPGe) detector, which was calibrated using gamma reference standard sources, was used to measure the activity of a radium solid source and radon decay products ((214)Bi or (214)Pb). The emanation factor of the (226)Ra source was controlled online with the HPGe detector. Radon activity was achieved at ~1500±45Bq from the radium source at 3.95±0.2kBq under equilibrium conditions. After this activity, the radon gas was transferred into the closed system producing radon activity concentrations of 31.1±0.3kBq/m(3). Systematic errors were found of less than 4% with a random error around 0.5%. The random error is generally associated with the estimation of the count rate of the measured radon progenies ((214)Po and (214)Po for alpha measurements or (214)Pb and (214)Bi for gamma measurements), but systematic errors are associated with the errors introduced by the instrumentation and measurement technique. The system that was developed has a high degree of accuracy and can be recommended as a national or regional prototype standard of radon activity concentration to calibrate different working radon measurement devices.

  15. Radon tightness of different sample sealing methods for gamma spectrometric measurements of ²²⁶Ra.

    Science.gov (United States)

    Mauring, Alexander; Gäfvert, Torbjörn

    2013-11-01

    Different methods for sealing sample containers for (222)Rn when measuring (226)Ra through its progenies (214)Pb and (214)Bi using gamma-ray spectrometry have been investigated. Results show that a method consisting of vacuum packaging of the sample container in a sealed aluminium lined bag gives excellent results for ensuring radon tightness. However, care should be taken to fill the sample container completely in order to avoid systematic errors due to radon accumulating in the void volume.

  16. Study on the solubility of radon in tissues; Untersuchung der Loeslichkeit von Radon in Gewebe

    Energy Technology Data Exchange (ETDEWEB)

    Fournier, Claudia; Kraft, Gerhard; Maier, Andreas; Beek, Patrick van [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, Darmstadt (Germany)

    2016-08-01

    At the GSI Helmholtz Center in Darmstadt a radon chamber with conditions similar to the radon galleries was built for studies on the solubility of radon in tissues using cell cultures and animals. The samples are investigated using gamma spectroscopy following the radon exposure measuring Pb-214 and Bi-214. The original concentration of Rn-222 in the sample is determined by the time dependence of the decay rates of Rn-222. The experimental conditions and preliminary measurements are described.

  17. AcEST: BP918061 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ssical arabinogalactan protein 3 OS=Arab... 33 1.4 sp|Q8WZ42|TITIN_HUMAN Titin OS=H... sapiens GN=SMG7 PE=1 SV=2 33 1.4 sp|P35658|NU214_HUMAN Nuclear pore complex protein Nup214 OS=Hom... 33 1.4 sp|Q9ZT17|AGP3_ARATH Cla

  18. Comparison of Alternative Modes of Data Input to the Pesticide Information Retrieval System.

    Science.gov (United States)

    1982-10-01

    residtr 6 no. of units & unit ? 214 msf 7 bldg/terrain ? res 8 pesticide ? diazinon 9 form ? eml 10 amount & unit ? 214 ga 11 final concentration ? 1 12...73344 NIseri liver "l101 Fort Indicators Cap 17003 Part" e. 92609 ber uEnad 02134 Fort Istri 32311 AM11 AF503-WO/00 North Aet lei 10007 Fort 3n guests

  19. 31 CFR 535.504 - Certain judicial proceedings with respect to property of Iran or Iranian entities.

    Science.gov (United States)

    2010-07-01

    ... contingent liabilities and property interests of the Government of Iran, its agencies, instrumentalities, or... any income earned thereon) subject to the provisions of §§ 535.211, 535.212, 535.213, 535.214 or 535... proceeding, pertaining to any property subject to the provisions of §§ 535.211, 535.212, 535.213, 535.214...

  20. Study of Rn-222 exhalation in phosphogypsum through the adsorption technique in activated coal; Estudo da exalacao de Rn-222 em fosfogesso por meio da tecnica de adsorcao em carvao ativado

    Energy Technology Data Exchange (ETDEWEB)

    Nisti, Marcelo Bessa; Campos, Marcia Pires de, E-mail: mbnisti@ipen.b, E-mail: mpcampos@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-10-26

    The radon exhalation was estimated through the adsorption in activated carbon technique. Classified as TENORM, the radon exhalation determination on the phosphogypsum piles was performed through the adsorption ratio of radon in activated carbon, from the concentration of descendants of {sup 222}Rn, {sup 214}Pb and {sup 214}Bi obtained by gamma spectrometry. The results obtained in this work were compatibles with the values found in the literature