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Sample records for actinium 212

  1. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-08-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac-Cl and Ac-OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1- ligands (3.2+/-1.1) than AmIII (0.8+/-0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour.

  2. Spectroscopic and computational investigation of actinium coordination chemistry

    Science.gov (United States)

    Ferrier, Maryline G.; Batista, Enrique R.; Berg, John M.; Birnbaum, Eva R.; Cross, Justin N.; Engle, Jonathan W.; La Pierre, Henry S.; Kozimor, Stosh A.; Lezama Pacheco, Juan S.; Stein, Benjamin W.; Stieber, S. Chantal E.; Wilson, Justin J.

    2016-01-01

    Actinium-225 is a promising isotope for targeted-α therapy. Unfortunately, progress in developing chelators for medicinal applications has been hindered by a limited understanding of actinium chemistry. This knowledge gap is primarily associated with handling actinium, as it is highly radioactive and in short supply. Hence, AcIII reactivity is often inferred from the lanthanides and minor actinides (that is, Am, Cm), with limited success. Here we overcome these challenges and characterize actinium in HCl solutions using X-ray absorption spectroscopy and molecular dynamics density functional theory. The Ac–Cl and Ac–OH2O distances are measured to be 2.95(3) and 2.59(3) Å, respectively. The X-ray absorption spectroscopy comparisons between AcIII and AmIII in HCl solutions indicate AcIII coordinates more inner-sphere Cl1– ligands (3.2±1.1) than AmIII (0.8±0.3). These results imply diverse reactivity for the +3 actinides and highlight the unexpected and unique AcIII chemical behaviour. PMID:27531582

  3. Discovery of the actinium, thorium, protactinium, and uranium isotopes

    CERN Document Server

    Fry, C

    2012-01-01

    Currently, 31 actinium, 31 thorium, 28 protactinium, and 23 uranium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  4. Production of high-purity radium-223 from legacy actinium-beryllium neutron sources.

    Science.gov (United States)

    Soderquist, Chuck Z; McNamara, Bruce K; Fisher, Darrell R

    2012-07-01

    Radium-223 is a short-lived alpha-particle-emitting radionuclide with potential applications in cancer treatment. Research to develop new radiopharmaceuticals employing (223)Ra has been hindered by poor availability due to the small quantities of parent actinium-227 available world-wide. The purpose of this study was to develop innovative and cost-effective methods to obtain high-purity (223)Ra from (227)Ac. We obtained (227)Ac from two surplus actinium-beryllium neutron generators. We retrieved the actinium/beryllium buttons from the sources and dissolved them in a sulfuric-nitric acid solution. A crude actinium solid was recovered from the solution by coprecipitation with thorium fluoride, leaving beryllium in solution. The crude actinium was purified to provide about 40 milligrams of actinium nitrate using anion exchange in methanol-water-nitric acid solution. The purified actinium was then used to generate high-purity (223)Ra. We extracted (223)Ra using anion exchange in a methanol-water-nitric acid solution. After the radium was separated, actinium and thorium were then eluted from the column and dried for interim storage. This single-pass separation produces high purity, carrier-free (223)Ra product, and does not disturb the (227)Ac/(227)Th equilibrium. A high purity, carrier-free (227)Th was also obtained from the actinium using a similar anion exchange in nitric acid. These methods enable efficient production of (223)Ra for research and new alpha-emitter radiopharmaceutical development.

  5. 48 CFR 212.212 - Computer software.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Computer software. 212.212... Acquisition of Commercial Items 212.212 Computer software. (1) Departments and agencies shall identify and... technology development), opportunities for the use of commercial computer software and other non...

  6. Relativistic small-core pseudopotentials for actinium, thorium, and protactinium.

    Science.gov (United States)

    Weigand, Anna; Cao, Xiaoyan; Hangele, Tim; Dolg, Michael

    2014-04-03

    Small-core pseudopotentials for actinium, thorium, and protactinium have been energy-adjusted to multiconfiguration Dirac-Hartree-Fock reference data based on the Dirac-Coulomb-Breit Hamiltonian and the Fermi nucleus model. Corresponding optimized valence basis sets of polarized valence quadruple-ζ quality are presented. Atomic test calculations for the first four ionization potentials show satisfactory results at both the Hartree-Fock and the multireference averaged coupled-pair functional level. Highly correlated Fock-space coupled cluster calculations demonstrate that the new pseudopotentials yield ionization potentials, which are in excellent agreement with corresponding all-electron results and experimental data. The pseudopotentials and basis sets supplement a similar set previously published for uranium.

  7. Effects of spin-orbit coupling on actinium under pressure

    Energy Technology Data Exchange (ETDEWEB)

    Rubio-Ponce, A.; Rivera, J. [Departamento de Ciencias Basicas, Universidad Autonoma Metropolitana-Azcapotzalco, Mexico (Mexico); Olguin, D. [Departamento de Fi sica, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Mexico (Mexico)

    2015-04-15

    Actinium (Ac) is a radioactive metal and the first element of the actinide series. At ambient conditions Ac crystallizes in the fcc lattice, however, up to date its phase diagram is unknown. In the present work, we have studied the structural and electronic properties of Ac under hydrostatic pressure assuming the fcc structure as well as three hypothetical structures, namely the hcp, bcc, and sc, and for pressures up to 100 GPa. From our calculations, we found only one structural transition allowed, from the fcc to hcp, our calculated pressure was 39.85 GPa. The calculations were performed by means of the full potential linearized augmented plane wave (FLAPW) method and the generalized gradient approximation (GGA) for the exchange-correlation energy, where we have included in our study the spin-orbit coupling which is important for heavy elements. The total energy results were fitted to the third order Birch-Murnaghan's equation of state. (copyright 2015 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  8. Production of Actinium-225 via High Energy Proton Induced Spallation of Thorium-232

    Energy Technology Data Exchange (ETDEWEB)

    Harvey, James T.; Nolen, Jerry; Vandergrift, George; Gomes, Itacil; Kroc, Tom; Horwitz, Phil; McAlister, Dan; Bowers, Del; Sullivan, Vivian; Greene, John

    2011-12-30

    The science of cancer research is currently expanding its use of alpha particle emitting radioisotopes. Coupled with the discovery and proliferation of molecular species that seek out and attach to tumors, new therapy and diagnostics are being developed to enhance the treatment of cancer and other diseases. This latest technology is commonly referred to as Alpha Immunotherapy (AIT). Actinium-225/Bismuth-213 is a parent/daughter alpha-emitting radioisotope pair that is highly sought after because of the potential for treating numerous diseases and its ability to be chemically compatible with many known and widely used carrier molecules (such as monoclonal antibodies and proteins/peptides). Unfortunately, the worldwide supply of actinium-225 is limited to about 1,000mCi annually and most of that is currently spoken for, thus limiting the ability of this radioisotope pair to enter into research and subsequently clinical trials. The route proposed herein utilizes high energy protons to produce actinium-225 via spallation of a thorium-232 target. As part of previous R and D efforts carried out at Argonne National Laboratory recently in support of the proposed US FRIB facility, it was shown that a very effective production mechanism for actinium-225 is spallation of thorium-232 by high energy proton beams. The base-line simulation for the production rate of actinium-225 by this reaction mechanism is 8E12 atoms per second at 200 MeV proton beam energy with 50 g/cm2 thorium target and 100 kW beam power. An irradiation of one actinium-225 half-life (10 days) produces {approx}100 Ci of actinium-225. For a given beam current the reaction cross section increases slightly with energy to about 400 MeV and then decreases slightly for beam energies in the several GeV regime. The object of this effort is to refine the simulations at proton beam energies of 400 MeV and above up to about 8 GeV. Once completed, the simulations will be experimentally verified using 400 MeV and 8 Ge

  9. Report for General Research September 18 to December 11, 1950 (Actinium Volume)

    Energy Technology Data Exchange (ETDEWEB)

    Haring, M.M.

    1951-01-15

    The purpose of the research work presented in this volume is to develop a process for the separation and purification of actinium-227 produced by neutron bombardment of radium-226 and to develop methods by which uniform films of actinium metal may be deposited on metallic surfaces. The design work on the cave structure and mechanical equipment used in the actinium separation is proceeding on schedule. As the mechanical design phase is nearing completion the emphasis is being directed toward processing equipment. The process as well as the mechanical equipment has been adapted from the research work of F. T. Hagemann and the Remote Control Group at Argonne National Laboratory. Consequently, one of the first objectives is to become familiary with the chemistry of the process and the operation of the mechanical equipment. Cold runs have been made on the T.T.A. benzene extraction using lanthanum and barium in place of actinium and radium. No difficulty with the operation was observed. The formation of precipitates was one of the difficulties encountered with the process as the precipitates carry radium. It has been found that metals such as nickel cause these precipitates to form and should, therefore, be avoided in the construction of equipment. it was also found that a T.T.A. solution exposed to 0.5 curie of polonium over a period of days develops a precipitate. Some new mechanical features hav eshown promise. The use of copper-coated glassware which will hold together even though the glass is cracked has made it possible to replace custom-built heaters with standard heating mantles. A new graphite, silicone grease mixture appears to hold up in stopcocks handling benzene and, as a result, may eliminate the necessary of entering the cave for regreasing. Tests on the preparation of dense concrete have given results which meet the shielding requirements for the cave. A strippable paint and tape combination has been studied and specified to provide for decontamination of

  10. Analysis of the gamma spectra of the uranium, actinium, and thorium decay series

    Energy Technology Data Exchange (ETDEWEB)

    Momeni, M.H.

    1981-09-01

    This report describes the identification of radionuclides in the uranium, actinium, and thorium series by analysis of gamma spectra in the energy range of 40 to 1400 keV. Energies and absolute efficiencies for each gamma line were measured by means of a high-resolution germanium detector and compared with those in the literature. A gamma spectroscopy method, which utilizes an on-line computer for deconvolution of spectra, search and identification of each line, and estimation of activity for each radionuclide, was used to analyze soil and uranium tailings, and ore.

  11. 49 CFR 571.212 - Standard No. 212; Windshield mounting.

    Science.gov (United States)

    2010-10-01

    ... portion of the windshield periphery on each side of the vehicle longitudinal centerline. S5.2Vehicles not... TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR VEHICLE SAFETY STANDARDS Federal Motor Vehicle Safety Standards § 571.212 Standard No. 212; Windshield mounting. S1. Scope. This...

  12. Application of ion exchange and extraction chromatography to the separation of actinium from proton-irradiated thorium metal for analytical purposes.

    Science.gov (United States)

    Radchenko, V; Engle, J W; Wilson, J J; Maassen, J R; Nortier, F M; Taylor, W A; Birnbaum, E R; Hudston, L A; John, K D; Fassbender, M E

    2015-02-06

    Actinium-225 (t1/2=9.92d) is an α-emitting radionuclide with nuclear properties well-suited for use in targeted alpha therapy (TAT), a powerful treatment method for malignant tumors. Actinium-225 can also be utilized as a generator for (213)Bi (t1/2 45.6 min), which is another valuable candidate for TAT. Actinium-225 can be produced via proton irradiation of thorium metal; however, long-lived (227)Ac (t1/2=21.8a, 99% β(-), 1% α) is co-produced during this process and will impact the quality of the final product. Thus, accurate assays are needed to determine the (225)Ac/(227)Ac ratio, which is dependent on beam energy, irradiation time and target design. Accurate actinium assays, in turn, require efficient separation of actinium isotopes from both the Th matrix and highly radioactive activation by-products, especially radiolanthanides formed from proton-induced fission. In this study, we introduce a novel, selective chromatographic technique for the recovery and purification of actinium isotopes from irradiated Th matrices. A two-step sequence of cation exchange and extraction chromatography was implemented. Radiolanthanides were quantitatively removed from Ac, and no non-Ac radionuclidic impurities were detected in the final Ac fraction. An (225)Ac spike added prior to separation was recovered at ≥ 98%, and Ac decontamination from Th was found to be ≥ 10(6). The purified actinium fraction allowed for highly accurate (227)Ac determination at analytical scales, i.e., at (227)Ac activities of 1-100 kBq (27 nCi to 2.7 μCi). Copyright © 2014 Elsevier B.V. All rights reserved.

  13. Thorium and actinium polyphosphonate compounds as bone-seeking alpha particle-emitting agents.

    Science.gov (United States)

    Henriksen, Gjermund; Bruland, Oyvind S; Larsen, Roy H

    2004-01-01

    The present study explores the use of alpha-particle-emitting, bone-seeking agents as candidates for targeted radiotherapy. Actinium and thorium 1,4,7,10 tetraazacyclododecane N,N',N'',N''' 1,4,7,10-tetra(methylene) phosphonic acid (DOTMP) and thorium-diethylene triamine N,N',N'' penta(methylene) phosphonic acid (DTMP) were prepared and their biodistribution evaluated in conventional Balb/C mice at four hours after injection. All three bone-seeking agents showed a high uptake in bone and a low uptake in soft tissues. Among the soft tissue organs, only kidney had a relatively high uptake. The femur/kidney ratios for 227Th-DTMP, 228-Ac-DOTMP and 227Th-DOTMP were 14.2, 7.6 and 6.0, respectively. A higher liver uptake of 228Ac-DOTMP was seen than for 227Th-DTMP and 227Th-DOTMP. This suggests that some demetallation of the 228Ac-DOTMP complex had occurred. The results indicate that 225Ac-DOTMP, 227Th-DOTMP and 227Th-DTMP have promising properties as potential therapeutic bone-seeking agents.

  14. Developments towards in-gas-jet laser spectroscopy studies of actinium isotopes at LISOL

    Energy Technology Data Exchange (ETDEWEB)

    Raeder, S., E-mail: s.raeder@gsi.de [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Helmholtz-Institut Mainz, 55128 Mainz (Germany); GSI Helmholtzzentrum für Schwerionenforschung GmbH, Planckstraße 1, 64291 Darmstadt (Germany); Bastin, B. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); Block, M. [Helmholtz-Institut Mainz, 55128 Mainz (Germany); GSI Helmholtzzentrum für Schwerionenforschung GmbH, Planckstraße 1, 64291 Darmstadt (Germany); Institut für Kernchemie, Johannes Gutenberg Universität, 55128 Mainz (Germany); Creemers, P. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Delahaye, P. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); Ferrer, R. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Fléchard, X. [LPC Caen, ENSICAEN, Université de Caen, CNRS/IN2P3, Caen (France); Franchoo, S. [Institute de Physique Nucléaire (IPN) d’Orsay, 91406 Orsay, Cedex (France); Ghys, L. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); SCK-CEN, Belgian Nuclear Research Center, Boeretang 200, 2400 Mol (Belgium); Gaffney, L.P.; Granados, C. [KU Leuven, Instituut voor Kern- en Stralingsfysica, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Heinke, R. [Institut für Physik, Johannes Gutenberg Universität, 55128 Mainz (Germany); Hijazi, L. [GANIL, CEA/DSM-CNRS/IN2P3, B.P. 55027, 14076 Caen (France); and others

    2016-06-01

    To study exotic nuclides at the borders of stability with laser ionization and spectroscopy techniques, highest efficiencies in combination with a high spectral resolution are required. These usually opposing requirements are reconciled by applying the in-gas-laser ionization and spectroscopy (IGLIS) technique in the supersonic gas jet produced by a de Laval nozzle installed at the exit of the stopping gas cell. Carrying out laser ionization in the low-temperature and low density supersonic gas jet eliminates pressure broadening, which will significantly improve the spectral resolution. This article presents the required modifications at the Leuven Isotope Separator On-Line (LISOL) facility that are needed for the first on-line studies of in-gas-jet laser spectroscopy. Different geometries for the gas outlet and extraction ion guides have been tested for their performance regarding the acceptance of laser ionized species as well as for their differential pumping capacities. The specifications and performance of the temporarily installed high repetition rate laser system, including a narrow bandwidth injection-locked Ti:sapphire laser, are discussed and first preliminary results on neutron-deficient actinium isotopes are presented indicating the high capability of this novel technique.

  15. 19 CFR 212.26 - Determination.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Determination. 212.26 Section 212.26 Customs... IMPLEMENTATION OF THE EQUAL ACCESS TO JUSTICE ACT Procedures for Considering Applications § 212.26 Determination. The presiding officer shall issue a recommended determination on the application within 90 days after...

  16. 48 CFR 212.211 - Technical data.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Technical data. 212.211 Section 212.211 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... Acquisition of Commercial Items 212.211 Technical data. The DoD policy for acquiring technical data...

  17. 25 CFR 212.20 - Leasing procedures.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Leasing procedures. 212.20 Section 212.20 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF ALLOTTED LANDS FOR MINERAL DEVELOPMENT How To Acquire Leases § 212.20 Leasing procedures. (a) Application for leases shall...

  18. 14 CFR 212.7 - Direct sales.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Direct sales. 212.7 Section 212.7... REGULATIONS CHARTER RULES FOR U.S. AND FOREIGN DIRECT AIR CARRIERS § 212.7 Direct sales. (a) Certificated and... direct sales Public Charters by a foreign air carrier upon a finding that such action is necessary in...

  19. 42 CFR 59.212 - Grantee accountability.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Grantee accountability. 59.212 Section 59.212 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.212 Grantee accountability....

  20. 32 CFR 212.5 - Responsibilities.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Responsibilities. 212.5 Section 212.5 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS...) INSTALLATIONS § 212.5 Responsibilities. (a) The Principal Deputy Under Secretary for Personnel and Readiness...

  1. Carolina Herrera 212 On Ice

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    炎夏酷暑,如何运用丰富的想象力,创造清凉绿洲?212香冰鸡尾酒,试图为恼人的酷暑降温,带来一丝难得的凉意。薄荷与木质香调的清新,唤醒都市人尘封的心扉,更为感官带来清凉刺激,清新薄荷引领了心灵的解放与纯净而散发着阳刚魅力的檀香,则唤醒你对于东方世界的记忆。

  2. 31 CFR 538.212 - Exempt transactions.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Exempt transactions. 538.212 Section 538.212 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... services to market, produce or co-produce, create or assist in the creation of information and...

  3. 48 CFR 212.7002 - Pilot program.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Pilot program. 212.7002... OF DEFENSE ACQUISITION PLANNING ACQUISITION OF COMMERCIAL ITEMS Pilot Program for Transition to Follow-On Contracting After Use of Other Transaction Authority 212.7002 Pilot program....

  4. 19 CFR 212.03 - Proceedings covered.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Proceedings covered. 212.03 Section 212.03 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE... fees and expenses related to those portions of the proceedings conducted for the consideration...

  5. 49 CFR 212.101 - Program principles.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Program principles. 212.101 Section 212.101 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... principles. (a) The purpose of the national railroad safety program is to promote safety in all areas...

  6. 40 CFR 86.212-94 - [Reserved

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false 86.212-94 Section 86.212-94 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF... Year Gasoline-Fueled New Light-Duty Vehicles, New Light-Duty Trucks and New Medium-Duty...

  7. 31 CFR 800.212 - Foreign entity.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Foreign entity. 800.212 Section 800.212 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... business is outside the United States or its equity securities are primarily traded on one or more...

  8. 42 CFR 93.212 - Inquiry.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Inquiry. 93.212 Section 93.212 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES HEALTH ASSESSMENTS AND HEALTH EFFECTS STUDIES OF HAZARDOUS SUBSTANCES RELEASES AND FACILITIES PUBLIC HEALTH SERVICE POLICIES ON RESEARCH...

  9. 31 CFR 575.212 - Effective dates.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Effective dates. 575.212 Section 575.212 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... Effective dates. The effective dates of the prohibitions and directives contained in this subpart B are...

  10. 19 CFR 212.25 - Further proceedings.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Further proceedings. 212.25 Section 212.25 Customs... proceedings. (a) Ordinarily, the determination of an award will be made on the basis of the written record... initiative, the presiding officer may in his or her discretion order further proceedings, such as an...

  11. 32 CFR 212.4 - Policy.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Policy. 212.4 Section 212.4 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS PROCEDURES AND....4 Policy. It is DoD policy, consistent with 32 CFR part 213, that procedures be established for...

  12. 49 CFR 212.107 - Certification.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Certification. 212.107 Section 212.107... Certification. (a) Scope. In the event the FRA and the State agency do not agree on terms for the participation... the Federal Railroad Safety Act of 1970, the State shall file an annual certification with respect...

  13. Dicty_cDB: SSI212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SS (Link to library) SSI212 (Link to dictyBase) - - - Contig-U16354-1 SSI212E (Link... to Original site) - - - - - - SSI212E 608 Show SSI212 Library SS (Link to library) Clone ID SSI212 (Link to...ycdb.biol.tsukuba.ac.jp/CSM/SS/SSI2-A/SSI212Q.Seq.d/ Representative seq. ID SSI21...2E (Link to Original site) Representative DNA sequence >SSI212 (SSI212Q) /CSM/SS/SSI2-A/SSI212Q.Seq.d/ TTGTT... E Sequences producing significant alignments: (bits) Value SSI212 (SSI212Q) /CSM/SS/SSI2-A/SSI212Q.Seq.d/ 1

  14. 48 CFR 12.212 - Computer software.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Computer software. 12.212... software. (a) Commercial computer software or commercial computer software documentation shall be acquired... required to— (1) Furnish technical information related to commercial computer software or commercial...

  15. 32 CFR 552.212 - Scope.

    Science.gov (United States)

    2010-07-01

    ... Installation of Aberdeen Proving Ground, Maryland § 552.212 Scope. (a) The provisions of this subpart apply to all elements of U.S. Army Garrison, Aberdeen Proving Ground (USAGAPG), and the supported organizations and activities on the Aberdeen and Edgewood Areas of Aberdeen Proving Ground. (b) The provisions...

  16. 49 CFR 212.215 - Locomotive inspector.

    Science.gov (United States)

    2010-10-01

    ... applicable sections of the Safety Glazing Standards (49 CFR part 223), Locomotive Safety Standards (49 CFR part 229), Safety Appliance Standards (49 CFR part 231) and Power Brake Standards (49 CFR part 232), to... 49 Transportation 4 2010-10-01 2010-10-01 false Locomotive inspector. 212.215 Section...

  17. Development and Testing of a 212Pb/212Bi Peptide for Targeting Metastatic Melanoma

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, Darrell R.

    2012-10-25

    The purpose of this project is to develop a new radiolabeled peptide for imaging and treating metastatic melanoma. The immunoconjugate consists of a receptor-specific peptide that targets melanoma cells. The beta-emitter lead-212 (half-life = 10.4 hours) is linked by coordination chemistry to the peptide. After injection, the peptide targets melanoma receptors on the surfaces of melanoma cells. Lead-212 decays to the alpha-emitter bismuth-212 (half-life = 60 minutes). Alpha-particles that hit melanoma cell nuclei are likely to kill the melanoma cell. For cancer cell imaging, the lead-212 is replaced by lead-203 (half-life = 52 hours). Lead-203 emits 279 keV photons (80.1% abundance) that can be imaged and measured for biodistribution analysis, cancer imaging, and quantitative dosimetry.

  18. 7 CFR 1160.212 - Influencing governmental action.

    Science.gov (United States)

    2010-01-01

    ....212 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Milk), DEPARTMENT OF AGRICULTURE FLUID MILK PROMOTION PROGRAM Fluid Milk Promotion Order National Fluid Milk Processor Promotion Board § 1160.212...

  19. 45 CFR 212.7 - Repayment to the United States.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Repayment to the United States. 212.7 Section 212... UNITED STATES CITIZENS RETURNED FROM FOREIGN COUNTRIES § 212.7 Repayment to the United States. (a) An..., any or all of the cost of such assistance to the United States, except insofar as it is...

  20. 22 CFR 212.1 - Statement of policy.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Statement of policy. 212.1 Section 212.1 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION General § 212.1 Statement of policy. (a) It is the policy of the United States Agency for International Development...

  1. 27 CFR 555.212 - Smoking and open flames.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false Smoking and open flames. 555.212 Section 555.212 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Storage § 555.212 Smoking...

  2. 49 CFR 195.212 - Bending of pipe.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Bending of pipe. 195.212 Section 195.212... PIPELINE Construction § 195.212 Bending of pipe. (a) Pipe must not have a wrinkle bend. (b) Each field bend must comply with the following: (1) A bend must not impair the serviceability of the pipe. (2) Each...

  3. 7 CFR 58.212 - Hopper or dump room.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Hopper or dump room. 58.212 Section 58.212 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards....212 Hopper or dump room. A separate room shall be provided for the transfer of bulk dry dairy...

  4. 7 CFR 1400.212 - Growers of hybrid seed.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Growers of hybrid seed. 1400.212 Section 1400.212... AND SUBSEQUENT CROP, PROGRAM, OR FISCAL YEARS Payment Eligibility § 1400.212 Growers of hybrid seed. The existence of a hybrid seed contract for a person or legal entity will not be taken into...

  5. 10 CFR 212.78 - Tertiary incentive crude oil.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Tertiary incentive crude oil. 212.78 Section 212.78 Energy DEPARTMENT OF ENERGY OIL MANDATORY PETROLEUM PRICE REGULATIONS Producers of Crude Oil § 212.78 Tertiary incentive crude oil. Annual prepaid expenses report. By January 31 of each year after 1980, the...

  6. 22 CFR 212.11 - Materials to be published.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Materials to be published. 212.11 Section 212... Federal Register § 212.11 Materials to be published. (a) USAID separately states and currently publishes... adopted as authorized by law, and statements of general policy or interpretations of general...

  7. 29 CFR 2.12 - Audiovisual coverage permitted.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Audiovisual coverage permitted. 2.12 Section 2.12 Labor Office of the Secretary of Labor GENERAL REGULATIONS Audiovisual Coverage of Administrative Hearings § 2.12 Audiovisual coverage permitted. The following are the types of hearings where the...

  8. 36 CFR 212.81 - Use by over-snow vehicles.

    Science.gov (United States)

    2010-07-01

    ...-snow vehicles on National Forest System roads and National Forest System trails and in areas on..., emergency, or law enforcement vehicle for emergency purposes; (3) Authorized use of any combat or combat... trails, and areas on National Forest System lands in §§ 212.52, 212.53, 212.54, 212.55, 212.56, and 212...

  9. 48 CFR 212.7003 - Technical data and computer software.

    Science.gov (United States)

    2010-10-01

    ... computer software. 212.7003 Section 212.7003 Federal Acquisition Regulations System DEFENSE ACQUISITION... data and computer software. For purposes of establishing delivery requirements and license rights for technical data under 227.7102 and for computer software under 227.7202, there shall be a rebuttable...

  10. 22 CFR 212.22 - Protection of personal privacy.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Protection of personal privacy. 212.22 Section... Information for Public Inspection and Copying § 212.22 Protection of personal privacy. To the extent required to prevent a clearly unwarranted invasion of personal privacy, USAID may delete identifying details...

  11. 9 CFR 93.212 - Manure from quarantined poultry.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Manure from quarantined poultry. 93.212 Section 93.212 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS IMPORTATION OF CERTAIN ANIMALS, BIRDS, FISH, AND POULTR...

  12. 20 CFR 212.3 - Crediting of military service.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Crediting of military service. 212.3 Section... MILITARY SERVICE § 212.3 Crediting of military service. In determining an individual's entitlement to an... of a calendar month during which the individual was in the active military service of the...

  13. 20 CFR 410.212 - Conditions of entitlement; child.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Conditions of entitlement; child. 410.212...; Filing of Claims and Evidence § 410.212 Conditions of entitlement; child. (a) An individual is entitled to benefits if such individual: (1) Is the child or stepchild (see § 410.330) of (i) a deceased...

  14. 25 CFR 212.34 - Individual tribal assignments excluded.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Individual tribal assignments excluded. 212.34 Section... ALLOTTED LANDS FOR MINERAL DEVELOPMENT How To Acquire Leases § 212.34 Individual tribal assignments... made pursuant to tribal constitutions or ordinances for the use of individual Indians and assignees...

  15. 42 CFR 457.212 - Disallowance of claims for FFP.

    Science.gov (United States)

    2010-10-01

    ...) Reconsideration procedures. The reconsideration procedures are those set forth in 45 CFR part 16. (d... 42 Public Health 4 2010-10-01 2010-10-01 false Disallowance of claims for FFP. 457.212 Section 457...; Reduction of Federal Medical Payments § 457.212 Disallowance of claims for FFP. (a) Notice of...

  16. MR Imaging Findings in Xp21.2 Duplication Syndrome

    Science.gov (United States)

    Whitehead, Matthew T; Helman, Guy; Gropman, Andrea L

    2016-01-01

    Xp21.2 duplication syndrome is a rare genetic disorder of undetermined prevalence and clinical relevance. As the use of chromosomal microarray has become first line for the work-up of childhood developmental delay, more gene deletions and duplications have been recognized. To the best of our knowledge, the imaging findings of Xp21.2 duplication syndrome have not been reported. We report a case of a 33 month-old male referred for developmental delay that was found to have an Xp21.2 duplication containing IL1RAPL1 and multiple midline brain malformations.

  17. An Improved SEL Test of the ADV212 Video Codec

    Science.gov (United States)

    Wilcox, Edward P.; Campola, Michael J.; Nadendla, Seshagiri; Kadari, Madhusudhan; Gigliuto, Robert A.

    2017-01-01

    Single-event effect (SEE) test data is presented on the Analog Devices ADV212. Focus is given to the test setup used to improve data quality and validate single-event latch-up (SEL) protection circuitry.

  18. Dicty_cDB: CHR212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHR212 (Link to dictyBase) - - - Contig-U10972-1 | Contig-U131... library) Clone ID CHR212 (Link to dictyBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig Contig-U109...72-1 | Contig-U13146-1 Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/C...SVETFACHVYGIHGIQKLLQYLSPDQVESIIASIKGKVIQLSKDNKGNYLIQS FLKQFSPEVNQFVCDATMGNIEEICTHKVGCTVVNRCIDNANPEQLEKLVDKIT...SVETFACHVYGIHGIQKLLQYLSPDQVESIIASIKGKVIQLSKDNKGNYLIQS FLKQFSPEVNQFVCDATMGNIEEICTHKVGCTVVNRCIDNANPEQLEKLVDKIT

  19. Synthesis and evaluation of tetraphosphonates labelled with {sup 212}Bi, {sup 212}Pb and {sup 165}Er

    Energy Technology Data Exchange (ETDEWEB)

    Hassfjell, S.P

    1997-08-01

    The main goal of this work has been to achieve a synthesis of radiolabelled phosphonates for the improvement of diagnostic and therapy of osteoblastic osteosarcoma and sclerotic bone metastases. {sup 212}Bi-DOTMP is shown to be an in vivo stable bone seeking radiopharmaceutical with a potential for a {alpha}-particle therapy of the above mentioned diseases. It has biodistribution characteristics similar to {sup 153}Sm-EDTMP, which is now formally approved in several countries, most recently in the US. {sup 212}Pb-DOTMP is also a promising candidate having the advantage of increasing the effective half life of the {alpha}-emitter, although loss of some of the in vivo generated {sup 212}Bi may be a problem. A generator has been developed for the production of the {alpha}-emitting radionuclide {sup 212}Bi and its parent nuclide {sup 212}Pb. The generator is based on the emanation of {sup 220}Rn from ({sup 228}Th)barium stearate. The decay product of {sup 220}Rn, {sup 212}Pb deposits on the walls of a polyethylene bottle, and can be wasted off with distilled water. The generator shows no leakage of any long-lived parent nuclides, is easy to operate and has a high degree of radiation safety.

  20. 5 CFR 551.212 - Foreign exemption criteria.

    Science.gov (United States)

    2010-01-01

    ... ADMINISTRATION UNDER THE FAIR LABOR STANDARDS ACT Exemptions and Exclusions § 551.212 Foreign exemption criteria. Foreign exemption means a provision of the Act under which the minimum wage, overtime, and child labor... labor provisions of the Act do not apply to any employee who spends all hours of work in a given...

  1. 27 CFR 24.212 - High fermentation wine.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false High fermentation wine. 24..., DEPARTMENT OF THE TREASURY LIQUORS WINE Production of Other Than Standard Wine § 24.212 High fermentation wine. High fermentation wine is wine made with the addition of sugar within the limitations prescribed...

  2. 36 CFR 212.4 - Construction and maintenance.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Construction and maintenance... TRAVEL MANAGEMENT Administration of the Forest Transportation System § 212.4 Construction and maintenance. (a) Construction and maintenance work on forest transportation facilities with appropriated...

  3. 10 CFR 1021.212 - Research, development, demonstration, and testing.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Research, development, demonstration, and testing. 1021... ACT IMPLEMENTING PROCEDURES DOE Decisionmaking § 1021.212 Research, development, demonstration, and testing. (a) This section applies to the adoption and application of programs that involve research...

  4. 40 CFR 98.212 - GHGs to report.

    Science.gov (United States)

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Miscellaneous Uses of Carbonate § 98.212 GHGs to report. You must report CO2 process emissions from all miscellaneous carbonate use at your facility as specified in this subpart....

  5. 36 CFR 212.2 - Forest transportation program.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Forest transportation program... TRAVEL MANAGEMENT Administration of the Forest Transportation System § 212.2 Forest transportation... public at the headquarters of that administrative unit. (b) Forest transportation atlas. A...

  6. 18 CFR 706.212 - Miscellaneous statutory provisions.

    Science.gov (United States)

    2010-04-01

    ... EMPLOYEE RESPONSIBILITIES AND CONDUCT Conduct and Responsibilities of Employees § 706.212 Miscellaneous statutory provisions. The attention of each employee is directed to the following statutory provisions: (a... Government Service. (b) Chapter 11 of Title 18, United States Code, relating to bribery, graft and...

  7. 48 CFR 833.212 - Contracting officer's duties upon appeal.

    Science.gov (United States)

    2010-10-01

    ... AFFAIRS GENERAL CONTRACTING REQUIREMENTS PROTESTS, DISPUTES, AND APPEALS Disputes and Appeals 833.212... construction contracts administered by the Office of Construction and Facilities Management, copies of the... sent to the Director, Office of Construction and Facilities Management.) (b) Within 20 days of receipt...

  8. 34 CFR 668.212 - Loan servicing appeals.

    Science.gov (United States)

    2010-07-01

    ... EDUCATION, DEPARTMENT OF EDUCATION STUDENT ASSISTANCE GENERAL PROVISIONS Cohort Default Rates § 668.212 Loan... Program lender or the Direct Loan Servicer, as defined in 34 CFR 685.102, failed to perform one or more of... identifies a sample that is large enough to derive an estimate, acceptable at a 95 percent confidence...

  9. 48 CFR 53.212 - Acquisition of commercial items.

    Science.gov (United States)

    2010-10-01

    ... (CONTINUED) CLAUSES AND FORMS FORMS Prescription of Forms 53.212 Acquisition of commercial items. SF 1449 (Rev. 3/2005), Solicitation/Contract/Order for Commercial Items. SF 1449 is prescribed for use in... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Acquisition of...

  10. 9 CFR 113.212 - Bursal Disease Vaccine, Killed Virus.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bursal Disease Vaccine, Killed Virus..., DEPARTMENT OF AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS Killed Virus Vaccines § 113.212 Bursal Disease Vaccine, Killed Virus. Bursal Disease...

  11. Tl-208, Pb-212, Bi-212, Ra-226 and Ac-228 adsorption onto polyhydroxyethylmethacrylate-bentonite composite

    Energy Technology Data Exchange (ETDEWEB)

    Akkaya, Recep [Cumhuriyet University, Chemistry Department, 58140 Sivas (Turkey); Ulusoy, Ulvi, E-mail: ulusoy@cumhuriyet.edu.tr [Cumhuriyet University, Chemistry Department, 58140 Sivas (Turkey)

    2012-02-01

    The adsorption of naturally occurring radionuclides ({sup 208}Tl{sup +}, {sup 212}Pb{sup 2+}, {sup 226}Ra{sup 2+}, {sup 212}Bi{sup 3+} and {sup 228}Ac{sup 3+}) onto Polyhydroxyethylmethacrylate-bentonite (PHEMA-B) composite was investigated. Experimentally obtained isotherms were evaluated with reference to Langmuir, Freundlich and Dubinin-Radushkevich (DR) models. The adsorption isotherms were L type of Giles classification proving that PHEMA-B had a high affinity adsorbent for the studied radionuclides. The Langmuir adsorption capacities (X{sub L}) were in the order of {sup 226}Ra (2.8 MBq kg{sup -1})>{sup 212}Bi (0.4 MBq kg{sup -1})>{sup 212}Pb (0.3 MBq kg{sup -1})>{sup 228}Ac and {sup 208}Tl (0.2 MBq kg{sup -1}). The adsorption process was physical via complex formation after proton exchanger for which the adsorption energies obtained from DR model was evidence. The enthalpy and entropy changes were positive and the negative free enthalpy change was proof for the spontaneity of adsorption. The reusability tests for PHEMA-B for five uses demonstrated that the adsorbent could be reused after complete recovery of the loaded radionuclide ions by 1 M HCl. The chemical structure of the composite did not change after the reuses and storage foregoing.

  12. Tl-208, Pb-212, Bi-212, Ra-226 and Ac-228 adsorption onto polyhydroxyethylmethacrylate-bentonite composite

    Science.gov (United States)

    Akkaya, Recep; Ulusoy, Ulvi

    2012-02-01

    The adsorption of naturally occurring radionuclides ( 208Tl +, 212Pb 2+, 226Ra 2+, 212Bi 3+ and 228Ac 3+) onto Polyhydroxyethylmethacrylate-bentonite (PHEMA-B) composite was investigated. Experimentally obtained isotherms were evaluated with reference to Langmuir, Freundlich and Dubinin-Radushkevich (DR) models. The adsorption isotherms were L type of Giles classification proving that PHEMA-B had a high affinity adsorbent for the studied radionuclides. The Langmuir adsorption capacities ( XL) were in the order of 226Ra (2.8 MBq kg -1)> 212Bi (0.4 MBq kg -1)> 212Pb (0.3 MBq kg -1)> 228Ac and 208Tl (0.2 MBq kg -1). The adsorption process was physical via complex formation after proton exchanger for which the adsorption energies obtained from DR model was evidence. The enthalpy and entropy changes were positive and the negative free enthalpy change was proof for the spontaneity of adsorption. The reusability tests for PHEMA-B for five uses demonstrated that the adsorbent could be reused after complete recovery of the loaded radionuclide ions by 1 M HCl. The chemical structure of the composite did not change after the reuses and storage foregoing.

  13. 8 CFR 212.5 - Parole of aliens into the United States.

    Science.gov (United States)

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Parole of aliens into the United States. 212.5 Section 212.5 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS DOCUMENTARY REQUIREMENTS: NONIMMIGRANTS; WAIVERS; ADMISSION OF CERTAIN INADMISSIBLE ALIENS; PAROLE § 212.5...

  14. 18 CFR 157.212 - Synthetic and liquefied natural gas facilities.

    Science.gov (United States)

    2010-04-01

    ... of the Natural Gas Act for Certain Transactions and Abandonment § 157.212 Synthetic and liquefied... liquefied natural gas and that are not “related jurisdictional natural gas facilities” as defined in § 153.2... natural gas facilities. 157.212 Section 157.212 Conservation of Power and Water Resources FEDERAL...

  15. 5 CFR 792.212 - What is the definition of a child care contractor?

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false What is the definition of a child care contractor? 792.212 Section 792.212 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL... Legislation and to Whom Does It Apply? § 792.212 What is the definition of a child care contractor? Section...

  16. 8 CFR 212.7 - Waiver of certain grounds of inadmissibility.

    Science.gov (United States)

    2010-01-01

    ... inadmissibility. 212.7 Section 212.7 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION... requirement of section 212(e) of the Act if his or her participation in an exchange program was financed in.../her spouse or child who is a citizen of the United States or a lawful permanent resident alien,...

  17. 20 CFR 725.212 - Conditions of entitlement; surviving spouse or surviving divorced spouse.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Conditions of entitlement; surviving spouse or surviving divorced spouse. 725.212 Section 725.212 Employees' Benefits EMPLOYMENT STANDARDS... Dependents (augmented Benefits) § 725.212 Conditions of entitlement; surviving spouse or surviving...

  18. 40 CFR 180.212 - S-Ethyl cyclohexylethylthio-carbamate; tolerances for residues.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false S-Ethyl cyclohexylethylthio-carbamate; tolerances for residues. 180.212 Section 180.212 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Tolerances § 180.212 S-Ethyl cyclohexylethylthio-carbamate; tolerances for residues. (a) General. Tolerances...

  19. 25 CFR 212.43 - Royalty rates for minerals other than oil and gas.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Royalty rates for minerals other than oil and gas. 212.43 Section 212.43 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF ALLOTTED LANDS FOR MINERAL DEVELOPMENT Rents, Royalties, Cancellations, and Appeals § 212.43 Royalty rates for minerals other than oil and...

  20. 23 CFR 636.212 - To what extent must tradeoff decisions be documented?

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false To what extent must tradeoff decisions be documented? 636.212 Section 636.212 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS DESIGN-BUILD CONTRACTING Selection Procedures, Award Criteria § 636.212...

  1. Direct observation of long-lived isomers in $^{212}$Bi

    CERN Document Server

    Chen, L; Geissel, H; Litvinov, Yu A; Beckert, K; Beller, P; Bosch, F; Boutin, D; Caceres, L; Carroll, J J; Cullen, D M; Cullen, I J; Franzke, B; Gerl, J; Górska, M; Jones, G A; Kishada, A; Knöbel, R; Kozhuharov, C; Kurcewicz, J; Litvinov, S A; Liu, Z; Mandal, S; Montes, F; Münzenberg, G; Nolden, F; Ohtsubo, T; Patyk, Z; Plaß, W R; Podolyák, Zs; Rigby, S; Saito, N; Saito, T; Scheidenberger, C; Simpson, E C; Shindo, M; Steck, M; Sun, B; Williams, S J; Weick, H; Winkler, M; Wollersheim, H -J; Yamaguchi, T

    2013-01-01

    Long-lived isomers in 212Bi have been studied following 238U projectile fragmentation at 670 MeV per nucleon. The fragmentation products were injected as highly charged ions into the GSI storage ring, giving access to masses and half-lives. While the excitation energy of the first isomer of 212Bi was confirmed, the second isomer was observed at 1478(30) keV, in contrast to the previously accepted value of >1910 keV. It was also found to have an extended Lorentz-corrected in-ring halflife >30 min, compared to 7.0(3) min for the neutral atom. Both the energy and half-life differences can be understood as being due a substantial, though previously unrecognised, internal decay branch for neutral atoms. Earlier shell-model calculations are now found to give good agreement with the isomer excitation energy. Furthermore, these and new calculations predict the existence of states at slightly higher energy that could facilitate isomer de-excitation studies.

  2. Single Event Effect Testing of the Analog Devices ADV212

    Science.gov (United States)

    Wilcox, Ted; Campola, Michael; Kadari, Madhu; Nadendla, Seshagiri R.

    2017-01-01

    The Analog Devices ADV212 was initially tested for single event effects (SEE) at the Texas AM University Cyclotron Facility (TAMU) in July of 2013. Testing revealed a sensitivity to device hang-ups classified as single event functional interrupts (SEFI), soft data errors classified as single event upsets (SEU), and, of particular concern, single event latch-ups (SEL). All error types occurred so frequently as to make accurate measurements of the exposure time, and thus total particle fluence, challenging. To mitigate some of the risk posed by single event latch-ups, circuitry was added to the electrical design to detect a high current event and automatically recycle power and reboot the device. An additional heavy-ion test was scheduled to validate the operation of the recovery circuitry and the continuing functionality of the ADV212 after a substantial number of latch-up events. As a secondary goal, more precise data would be gathered by an improved test method, described in this test report.

  3. 22 CFR 212.37 - Procedures for agency consideration of appeals.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Procedures for agency consideration of appeals. 212.37 Section 212.37 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT PUBLIC INFORMATION Access... of the decisions and of the provisions for judicial review of the Agency's denial of the request. (c...

  4. 27 CFR 25.212 - Beer returned to brewery from which removed.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Beer returned to brewery from which removed. 25.212 Section 25.212 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO... against the quantity of beer removed for consumption or sale from the brewery on that business day,...

  5. 25 CFR 212.23 - Corporate qualifications and requests for information.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Corporate qualifications and requests for information. 212.23 Section 212.23 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS... and requests for information. The provisions of § 211.23 of this subchapter are applicable to leases...

  6. 23 CFR 973.212 - Indian lands safety management system (SMS).

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Indian lands safety management system (SMS). 973.212... HIGHWAYS MANAGEMENT SYSTEMS PERTAINING TO THE BUREAU OF INDIAN AFFAIRS AND THE INDIAN RESERVATION ROADS PROGRAM Bureau of Indian Affairs Management Systems § 973.212 Indian lands safety management system...

  7. 48 CFR 52.212-1 - Instructions to Offerors-Commercial Items.

    Science.gov (United States)

    2010-10-01

    ... evaluate compliance with the requirements in the solicitation. This may include product literature, or... certifications at FAR 52.212-3 (see FAR 52.212-3(b) for those representations and certifications that the offeror..., or maintenance. (j) Data Universal Numbering System (DUNS) Number. (Applies to all offers exceeding...

  8. 36 CFR 212.55 - Criteria for designation of roads, trails, and areas.

    Science.gov (United States)

    2010-07-01

    ..., conflicts among uses of National Forest System lands, the need for maintenance and administration of roads... roads, trails, and areas. 212.55 Section 212.55 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle...

  9. 36 CFR 212.9 - Principles for sharing use of roads.

    Science.gov (United States)

    2010-07-01

    ...; or (5) A combination of the aforementioned methods. (f) Road maintenance and resurfacing. Cooperators will share the road maintenance and resurfacing costs under suitable agreements to perform, arrange for... roads. 212.9 Section 212.9 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF...

  10. 31 CFR 585.212 - Prohibited transactions related to scientific and technical cooperation, cultural exchanges, and...

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Prohibited transactions related to scientific and technical cooperation, cultural exchanges, and other official visits. 585.212 Section 585.212 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE...

  11. 21 CFR 212.110 - How must I maintain records of my production of PET drugs?

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false How must I maintain records of my production of PET drugs? 212.110 Section 212.110 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... of PET drugs? (a) Record availability. Records must be maintained at the PET drug production facility...

  12. 24 CFR 5.212 - Compliance with the Privacy Act and other requirements.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Compliance with the Privacy Act and other requirements. 5.212 Section 5.212 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development GENERAL HUD PROGRAM REQUIREMENTS; WAIVERS Disclosure...

  13. 25 CFR 212.6 - Authority and responsibility of the Minerals Management Service (MMS).

    Science.gov (United States)

    2010-04-01

    ... the Minerals Management Service (MMS). The functions of the MMS for reporting, accounting, and... 25 Indians 1 2010-04-01 2010-04-01 false Authority and responsibility of the Minerals Management Service (MMS). 212.6 Section 212.6 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND...

  14. 25 CFR 212.46 - Inspection of premises, books, and accounts.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Inspection of premises, books, and accounts. 212.46 Section 212.46 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF... premises, books, and accounts. The provisions of § 211.46 of this subchapter are applicable to leases...

  15. 8 CFR 212.12 - Parole determinations and revocations respecting Mariel Cubans.

    Science.gov (United States)

    2010-01-01

    ... respecting Mariel Cubans. 212.12 Section 212.12 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY... health; (iv) Institutional progress relating to participation in work, educational and vocational... sibling who is a lawful permanent resident or a citizen of the United States. (g) Timing of reviews....

  16. 25 CFR 212.47 - Diligence, drainage and prevention of waste.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Diligence, drainage and prevention of waste. 212.47 Section 212.47 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF..., drainage and prevention of waste. The provisions of § 211.47 of this subchapter are applicable to...

  17. 20 CFR 702.212 - Notice; when given; when given for certain occupational diseases.

    Science.gov (United States)

    2010-04-01

    ... occupational diseases. 702.212 Section 702.212 Employees' Benefits EMPLOYMENT STANDARDS ADMINISTRATION... occupational diseases. (a) For other than occupational diseases described in (b), the employee must give notice...). (b) In the case of an occupational disease which does not immediately result in disability or...

  18. 21 CFR 212.10 - What personnel and resources must I have?

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What personnel and resources must I have? 212.10.... 12-12-2011) Personnel and Resources § 212.10 What personnel and resources must I have? You must have... to perform their assigned functions. You must have adequate resources, including facilities...

  19. Tribology and Microstructure of PS212 with a Cr2O3 Seal Coat

    Science.gov (United States)

    Sliney, Harold E.; Benoy, Patricia A.; Korenyi-Both, Andras; Dellacorte, Christopher

    1994-01-01

    PS212 is a plasma sprayed metal bonding chrome carbide coating with solid lubricant additives which has lubricating properties at temperatures up to about 900 deg C. The coating is diamond ground to achieve an acceptable tribological surface. But, as with many plasma spray coatings, PS212 is not fully-dense. In this study, a chromium oxide base seal coating is used in an attempt to seal any porosity that is open to the surface of the PS212 coating, and to study the effect of the sealant on the tribological properties of PS212. The results indicate that the seal coating reduces friction and wear when it is applied and then diamond ground leaving a thin layer of seal coating which fills in the surface pits of the PS212 coating.

  20. The tribology of PS212 coatings and PM212 composites for the lubrication of titanium 6Al-4V components of a Stirling engine space power system

    Science.gov (United States)

    Sliney, Harold E.; Lukaszewicz, Victor; Dellacorte, Christopher

    1994-01-01

    The Stirling space power machine incorporates a linear alternator to generate electrical power. The alternator is a reciprocating device that is driven by a solar or nuclear-powered Stirling engine. The power piston and cylinder are made of titanium 6Al-4V (Ti6-4) alloy, and are designed to be lubricated by a hydrodynamically-generated gas film. Rubbing occurs during starts and stops and there is the possibility of an occasional high speed rub. Since titanium is known to have a severe galling tendency in sliding contacts, a 'back-up', self-lubricating coating on the cylinder and/or the piston is needed. This report describes the results of a research program to study the lubrication of Ti6-4 with the following chromium carbide based materials: plasma-sprayed PS212 coatings and sintered PM212 counterfaces. Program objectives are to achieve adherent coatings on Ti6-4 and to measure the friction and wear characteristics of the following sliding combinations under conditions simulative of the Stirling-driven space power linear alternator: Ti6-4/Ti6-4 baseline, Ti6-4/PS212-coated Ti6-4, and PS212-coated Ti6-4/PM212.

  1. DMU-212 inhibits tumor growth in xenograft model of human ovarian cancer.

    Science.gov (United States)

    Piotrowska, Hanna; Myszkowski, Krzysztof; Abraszek, Joanna; Kwiatkowska-Borowczyk, Eliza; Amarowicz, Ryszard; Murias, Marek; Wierzchowski, Marcin; Jodynis-Liebert, Jadwiga

    2014-05-01

    DMU-212 has been shown to evoke a mitochondrial apoptotic pathway in transformed fibroblasts and breast cancer. However, recently published data indicated the ability of DMU-212 to evoke apoptosis in both mitochondria- and receptor-mediated manner in two ovarian cancer cell lines, namely A-2780 and SKOV-3, which showed varied sensitivity to the compound tested. The pronounced cytotoxic effects of DMU-212 observed in A-2780 cells were related to the execution of extracellular apoptosis pathway and cell cycle arrest in G2/M phase. In view of the great anticancer potential of DMU-212 against A-2780 cell line, the aim of the current study was to assess antiproliferative activity of DMU-212 in xenograft model of ovarian cancer. To evaluate in vitro metabolic properties of cells that were to be injected into SCID mice, uptake and decline of DMU-212 in A-2780 ovarian cancer cell line was investigated. It was found that the concentration of the test compound in A-2780 cells was growing within first eight hours, and then the gradual decline was observed. A-2780 cells stably transfected with pcDNA3.1/Zeo(-)-Luc vector were subcutaneously inoculated into the right flanks of SCID mice. After seven days of the treatment with DMU-212 (50mg/kg b.w), tumor growth appeared to be suppressed in the animals treated with the compound tested. At day 14 of the experiment, tumor burden in mice treated with DMU-212 was significantly lower, as compared to untreated controls. Our findings suggest that DMU-212 might be considered as a potential anticancer agent used in ovarian cancer therapy. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  2. 48 CFR 212.503 - Applicability of certain laws to executive agency contracts for the acquisition of commercial items.

    Science.gov (United States)

    2010-10-01

    ... laws to executive agency contracts for the acquisition of commercial items. 212.503 Section 212.503... Commercial Items 212.503 Applicability of certain laws to executive agency contracts for the acquisition of commercial items. (a) The following laws are not applicable to contracts for the acquisition of...

  3. 42 CFR 435.212 - Individuals who would be ineligible if they were not enrolled in an MCO or PCCM.

    Science.gov (United States)

    2010-10-01

    ... not enrolled in an MCO or PCCM. 435.212 Section 435.212 Public Health CENTERS FOR MEDICARE & MEDICAID... Disabled § 435.212 Individuals who would be ineligible if they were not enrolled in an MCO or PCCM. The State agency may provide that a recipient who is enrolled in an MCO or PCCM and who becomes...

  4. 47 CFR 90.212 - Provisions relating to the use of scrambling devices and digital voice modulation.

    Science.gov (United States)

    2010-10-01

    ... devices and digital voice modulation. 90.212 Section 90.212 Telecommunication FEDERAL COMMUNICATIONS... Standards § 90.212 Provisions relating to the use of scrambling devices and digital voice modulation. (a... emission, subject to the provision of paragraph (d) of this section. (b) The use of digital scrambling...

  5. Aptamer-miRNA-212 Conjugate Sensitizes NSCLC Cells to TRAIL

    Directory of Open Access Journals (Sweden)

    Margherita Iaboni

    2016-01-01

    Full Text Available TNF-related apoptosis-inducing ligand (TRAIL is a promising antitumor agent for its remarkable ability to selectively induce apoptosis in cancer cells, without affecting the viability of healthy bystander cells. The TRAIL tumor suppressor pathway is deregulated in many human malignancies including lung cancer. In human non-small cell lung cancer (NSCLC cells, sensitization to TRAIL therapy can be restored by increasing the expression levels of the tumor suppressor microRNA-212 (miR-212 leading to inhibition of the anti-apoptotic protein PED/PEA-15 implicated in treatment resistance. In this study, we exploited a previously described RNA aptamer inhibitor of the tyrosine kinase receptor Axl (GL21.T expressed on lung cancer cells, as a means to deliver miR-212 into human NSCLC cells expressing Axl. We demonstrate efficient delivery of miR-212 following conjugation of the miR to GL21.T (GL21.T-miR212 chimera. We show that the chimera downregulates PED and restores TRAIL-mediate cytotoxicity in cancer cells. Importantly, treatment of Axl+ lung cancer cells with the chimera resulted in (i an increase in caspase activation and (ii a reduction of cell viability in combination with TRAIL therapy. In conclusion, we demonstrate that the GL21.T-miR212 chimera can be employed as an adjuvant to TRAIL therapy for the treatment of lung cancer.

  6. Current Sensor Based on Magnetic Sensor MMC212xMG%基于MMC212xMG的电流传感器

    Institute of Scientific and Technical Information of China (English)

    熊显名; 莫荣军

    2012-01-01

    针对传统电流测量方法精度不高、工艺复杂等问题,利用各向异性磁阻传感器MMC212xMG优异的磁场测量性能设计了一种高精度非接触式电流传感器.该电流传感器以磁场传感器MMC212xMG作为核心检测部件,ATmega32微控制器作为主控器,实时测量磁场数据,对数据进行滤波和计算后显示电流值.被测电流与磁场传感器MMC212xMG都处在密闭的坡莫合金屏蔽体内,有效避免外界磁场对电流磁检测过程的干扰.实验结果表明,该电流传感器具有精度高、非接触式测量、稳定性好、体积小等特点.%For the accuracy of conventional current measurement methods is not high and their processes are complex,in the basis of the excellent magnetic field measurement performance of anisotropic magnetoresistive sensors MMC212xMG,one high-precision non-contact current sensor was designed. Taking the magnetic field sensor MMC212xMG as a core detecting component and ATmega32 microcontroller as a master,the sensor system detected magnetic field data in real-time,filtered the data and displayed the current value after mathematical operation. The measured current and magnetic field sensor MMC212xMG were in sealed permalloy shielding body to avoid the interference of external magnetic field on current magnetic detection process effectively. Experimental results show that the system has the characteristics of high accuracy, non-contact measurement, good stability, and small size.

  7. The miRNA-212/132 family regulates both cardiac hypertrophy and cardiomyocyte autophagy

    Science.gov (United States)

    Ucar, Ahmet; Gupta, Shashi K.; Fiedler, Jan; Erikci, Erdem; Kardasinski, Michal; Batkai, Sandor; Dangwal, Seema; Kumarswamy, Regalla; Bang, Claudia; Holzmann, Angelika; Remke, Janet; Caprio, Massimiliano; Jentzsch, Claudia; Engelhardt, Stefan; Geisendorf, Sabine; Glas, Carolina; Hofmann, Thomas G.; Nessling, Michelle; Richter, Karsten; Schiffer, Mario; Carrier, Lucie; Napp, L. Christian; Bauersachs, Johann; Chowdhury, Kamal; Thum, Thomas

    2012-01-01

    Pathological growth of cardiomyocytes (hypertrophy) is a major determinant for the development of heart failure, one of the leading medical causes of mortality worldwide. Here we show that the microRNA (miRNA)-212/132 family regulates cardiac hypertrophy and autophagy in cardiomyocytes. Hypertrophic stimuli upregulate cardiomyocyte expression of miR-212 and miR-132, which are both necessary and sufficient to drive the hypertrophic growth of cardiomyocytes. MiR-212/132 null mice are protected from pressure-overload-induced heart failure, whereas cardiomyocyte-specific overexpression of the miR-212/132 family leads to pathological cardiac hypertrophy, heart failure and death in mice. Both miR-212 and miR-132 directly target the anti-hypertrophic and pro-autophagic FoxO3 transcription factor and overexpression of these miRNAs leads to hyperactivation of pro-hypertrophic calcineurin/NFAT signalling and an impaired autophagic response upon starvation. Pharmacological inhibition of miR-132 by antagomir injection rescues cardiac hypertrophy and heart failure in mice, offering a possible therapeutic approach for cardiac failure. PMID:23011132

  8. Memory formation and retention are affected in adult miR-132/212 knockout mice.

    Science.gov (United States)

    Hernandez-Rapp, Julia; Smith, Pascal Y; Filali, Mohammed; Goupil, Claudia; Planel, Emmanuel; Magill, Stephen T; Goodman, Richard H; Hébert, Sébastien S

    2015-01-01

    The miR-132/212 family is thought to play an important role in neural function and plasticity, while its misregulation has been observed in various neurodegenerative disorders. In this study, we analyzed 6-month-old miR-132/212 knockout mice in a battery of cognitive and non-cognitive behavioral tests. No significant changes were observed in reflexes and basic sensorimotor functions as determined by the SHIRPA primary screen. Accordingly, miR-132/212 knockout mice did not differ from wild-type controls in general locomotor activity in an open-field test. Furthermore, no significant changes of anxiety were measured in an elevated plus maze task. However, the mutant mice showed retention phase defects in a novel object recognition test and in the T-water maze. Moreover, the learning and probe phases in the Barnes maze were clearly altered in knockout mice when compared to controls. Finally, changes in BDNF, CREB, and MeCP2 were identified in the miR-132/212-deficient mice, providing a potential mechanism for promoting memory loss. Taken together, these results further strengthen the role of miR-132/212 in memory formation and retention, and shed light on the potential consequences of its deregulation in neurodegenerative diseases.

  9. Free radical detection by means of {sup 2}12Pb mirrors; Deteccion de radicales libres mediante espejos de Pb{sup 2}12

    Energy Technology Data Exchange (ETDEWEB)

    Molera, M.J.; Gamboa, J. M.; Garcia Guijarro, E.

    1961-07-01

    Radicals have been detected at low temperatures (550-700 degree centigrade) in the thermal decomposition of dimethyl and methyl-ethyl-ketone by the Paneth method. the sensitivity of the method has been increased by the use of radioactive mirrors of PB{sup 2}12. They have proved to detect one radical among 2.10{sup 6} molecules. (Author) 18 refs.

  10. The effect of processing and compositional changes on the tribology of PM212 in air

    Science.gov (United States)

    Bogdanski, Michael S.; Sliney, Harold E.; Dellacorte, Christopher

    1993-01-01

    The effects of processing and compositional variations on the tribological performance of PM212 were studied. PM212 is a self lubricating powder metallurgy composite, comprised of a wear resistant metal bonded chromium carbide matrix, containing the solid lubricants barium fluoride/calcium fluoride eutectic and silver. Several composites were formulated which had lubricant, matrix, and processing variations. Processing variations included sintering and hot isostatic pressing. Pins fabricated from the composites were slid against superalloys disks in a pin-on-disk tribometer to study the tribological properties. Several composites exhibited low friction and wear in sliding against a nickel based superalloy. The tribological performance by several different composites showed that the composition of PM212 can be altered without dramatically affecting performance.

  11. Inhibitory effects of synthetic cannabinoid WIN55, 212-2 on nicotine-activated currents in rat trigeminal ganglion neurons

    Institute of Scientific and Technical Information of China (English)

    Yongli Lu; Changjin Liu; Hongwei Yang

    2011-01-01

    Cannabinoid and nicotinic acetylcholine receptors are strongly associated with algesia. Previous studies in our laboratory have reported inhibitory effects of synthetic cannabinoid WIN55, 212-2 on nicotine-activated currents (/nic), but the underlying mechanisms remain poorly understood. The present study used whole-cell patch clamp techniques to investigate the modulatory effects of synthetic cannabinoid WIN55, 212-2 on /nic in cultured rat trigeminal ganglion neurons. The results revealed several major findings: WIN55, 212-2 inhibited /nic in rat trigeminal ganglion neurons. In addition, when WIN55, 212-2 (3 μmol/L) was applied simultaneously with nicotine (100 μmol/L), the inhibition of WIN55, 212-2 on /nic was reversible, concentration-dependent and voltage-independent. This effect was not mediated by CB1, CB2 or VR1 receptors; neither the selective CB1 receptor antagonist AM281, CB2 receptor antagonist AM630 nor VR1 receptor antagonist capsazepine reduced the inhibitory effect of WIN55, 212-2. Further, the inhibition of nicotinic responses by WIN55, 212-2 was not sensitive to the membrane permeable cyclic adenosine monophosphate (cAMP) analog 8-Br-cAMP. The G-protein inhibitor GDP-β-S (1 mmol/L) did not block the inhibitory effects of WIN55, 212-2 on /nic, excluding the involvement of G-protein mediation. The results suggested that WIN55, 212-2 inhibits/nic directly via the neuronal nicotinic acetylcholine receptor, and that this inhibition is non-competitive. WIN55, 212-2 did not act as an open channel blocker of the neuronal nicotinic acetylcholine receptor, and did not affect the desensitization of /nic. The results suggest that nicotine receptors may be physically plugged from outside the membrane by drugs containing WIN55, 212-2.

  12. Pretreatment with clonidine caused desensitization to WIN 55,212-2 in guinea pig ileum.

    Science.gov (United States)

    Rezania, F; Mohaghegh Shalmani, L; Rahimian, R; Dehpour, A R; Ejtemaei Mehr, S

    2014-01-01

    Considering the existence of cross-tolerance between clonidine and morphine besides the same interaction between morphine and WIN 55,212-2 persuaded us to verify this fact between WIN 55,212-2 and clonidine in guinea pig ileum, which is a well-known model to examine the mode of action of cannabinoids and α2 -adenoceptor agonists The rectangular pulses were passed to the 0.5 g stretched ileum segments that were fixed in 20-ml organ bath. PowerLab system and Graphpad Prism were applied to record twitches and analyse the data. Electrically evoked contractions were dose-dependently inhibited by WIN 55,212-2 and clonidine (pD2 = 8.56 ± 0.41 and 7.65 ± 0.15, respectively). Tolerance to this effect could be induced by 4-h incubation with WIN 55,212-2 (3 × IC50 ) (pD2  = 6.36 ± 0.26, degree of tolerance: 159.32) (P systems interaction in the enteric nervous system as a simplified representative for central nervous system.

  13. Tribological properties of PM212: A high-temperature, self-lubricating, powder metallurgy composite

    Science.gov (United States)

    Dellacorte, Christopher; Sliney, Harold E.

    1989-01-01

    This paper describes a research program to develop and evaluate a new high temperature, self-lubricating powder metallurgy composite, PM212. PM212 has the same composition as the plasma-sprayed coating, PS212, which contains 70 wt percent metal-bonded chromium carbide, 15 wt percent silver and 15 wt percent barium fluoride/calcium fluoride eutectic. The carbide acts as a wear resistant matrix and the silver and fluorides act as low and high temperature lubricants, respectively. The material is prepared by sequential cold press, cold isostatic pressing and sintering techniques. In this study, hemispherically tipped wear pins of PM212 were prepared and slid against superalloy disks at temperatures from 25 to 850 C in air in a pin-on-disk tribometer. Friction coefficients range from 0.29 to 0.38 and the wear of both the composite pins and superalloy disks was moderate to low in the 10(exp -5) to 10(exp -6) cubic mm/N-m range. Preliminary tests indicate that the material has a compressive strength of at least 130 MPa over the entire temperature range of 25 to 900 C. This material has promise for use as seal inserts, bushings, small inside diameter parts and other applications where plasma-sprayed coatings are impractical or too costly.

  14. 21 CFR 212.50 - What production and process controls must I have?

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What production and process controls must I have... DRUGS (Eff. 12-12-2011) Production and Process Controls § 212.50 What production and process controls must I have? You must have adequate production and process controls to ensure the consistent...

  15. 19 CFR 351.212 - Assessment of antidumping and countervailing duties; provisional measures deposit cap; interest...

    Science.gov (United States)

    2010-04-01

    ... duties; provisional measures deposit cap; interest on certain overpayments and underpayments. 351.212... countervailing duties; provisional measures deposit cap; interest on certain overpayments and underpayments. (a... regarding the assessment of duties, the provisional measures deposit cap, and interest on over- or...

  16. 36 CFR 212.51 - Designation of roads, trails, and areas.

    Science.gov (United States)

    2010-07-01

    ... AGRICULTURE TRAVEL MANAGEMENT Designation of Roads, Trails, and Areas for Motor Vehicle Use § 212.51 Designation of roads, trails, and areas. (a) General. Motor vehicle use on National Forest System roads, on... administrative use by the Forest Service; (5) Use of any fire, military, emergency, or law enforcement...

  17. Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination.

    Science.gov (United States)

    Qiao, Huanyu; Prasada Rao, H B D; Yang, Ye; Fong, Jared H; Cloutier, Jeffrey M; Deacon, Dekker C; Nagel, Kathryn E; Swartz, Rebecca K; Strong, Edward; Holloway, J Kim; Cohen, Paula E; Schimenti, John; Ward, Jeremy; Hunter, Neil

    2014-02-01

    Crossover recombination facilitates the accurate segregation of homologous chromosomes during meiosis. In mammals, poorly characterized regulatory processes ensure that every pair of chromosomes obtains at least one crossover, even though most recombination sites yield non-crossovers. Designation of crossovers involves selective localization of the SUMO ligase RNF212 to a minority of recombination sites, where it stabilizes pertinent factors such as MutSγ (ref. 4). Here we show that the ubiquitin ligase HEI10 (also called CCNB1IP1) is essential for this crossover/non-crossover differentiation process. In HEI10-deficient mice, RNF212 localizes to most recombination sites, and dissociation of both RNF212 and MutSγ from chromosomes is blocked. Consequently, recombination is impeded, and crossing over fails. In wild-type mice, HEI10 accumulates at designated crossover sites, suggesting that it also has a late role in implementing crossing over. As with RNF212, dosage sensitivity for HEI10 indicates that it is a limiting factor for crossing over. We suggest that SUMO and ubiquitin have antagonistic roles during meiotic recombination that are balanced to effect differential stabilization of recombination factors at crossover and non-crossover sites.

  18. 33 CFR 157.212 - Dedicated Clean Ballast Tanks Operations Manual: Not approved.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Dedicated Clean Ballast Tanks... RELATING TO TANK VESSELS CARRYING OIL IN BULK Dedicated Clean Ballast Tanks on Tank Vessels General § 157.212 Dedicated Clean Ballast Tanks Operations Manual: Not approved. If the Dedicated Clean...

  19. 48 CFR 212.270 - Major weapon systems as commercial items.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Major weapon systems as... Requirements for the Acquisition of Commercial Items 212.270 Major weapon systems as commercial items. The DoD policy for acquiring major weapon systems as commercial items is in Subpart 234.70. ...

  20. Yeast Interacting Proteins Database: YDL167C, YBR212W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available as bait (2) Rows with this bait as prey (0) YBR212W NGR1 RNA binding protein that negatively regulates grow...ption RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondr

  1. Tribological properties of PM212 - A high temperature, self-lubricating, powder metallurgy composite

    Science.gov (United States)

    Dellacorte, Christopher; Sliney, Harold E.

    1990-01-01

    This paper describes a research program to develop and evaluate a new high temperature, self-lubricating powder metallurgy composite, PM212. PM212 has the same composition as the plasma-sprayed coating, PS212, which contains 70 wt percent metal-bonded chromium carbide, 15 wt percent silver and 15 wt percent barium fluoride/calcium fluoride eutectic. The carbide acts as a wear resistant matrix and the silver and fluorides act as low and high temperature lubricants, respectively. The material is prepared by sequential cold press, cold isostatic pressing and sintering techniques. In this study, hemispherically tipped wear pins of PM212 were prepared and slid against superalloy disks at temperatures from 25 to 850 C in air in a pin-on-disk tribometer. Friction coefficients range from 0.29 to 0.38 and the wear of both the composite pins and superalloy disks was moderate to low in the 10(exp -5) to 10(exp -6) cubic mm/N-m range. Preliminary tests indicate that the material has a compressive strength of at least 130 MPa over the entire temperature range of 25 to 900 C. This material has promise for use as seal inserts, bushings, small inside diameter parts and other applications where plasma-sprayed coatings are impractical or too costly.

  2. Targeted Deletion of miR-132/-212 Impairs Memory and Alters the Hippocampal Transcriptome

    Science.gov (United States)

    Hansen, Katelin F.; Sakamoto, Kensuke; Aten, Sydney; Snider, Kaitlin H.; Loeser, Jacob; Hesse, Andrea M.; Page, Chloe E.; Pelz, Carl; Arthur, J. Simon C.; Impey, Soren; Obrietan, Karl

    2016-01-01

    miR-132 and miR-212 are structurally related microRNAs that have been found to exert powerful modulatory effects within the central nervous system (CNS). Notably, these microRNAs are tandomly processed from the same noncoding transcript, and share a common seed sequence: thus it has been difficult to assess the distinct contribution of each…

  3. 21 CFR 212.2 - What is current good manufacturing practice for PET drugs?

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What is current good manufacturing practice for... HUMAN SERVICES (CONTINUED) DRUGS: GENERAL CURRENT GOOD MANUFACTURING PRACTICE FOR POSITRON EMISSION TOMOGRAPHY DRUGS (Eff. 12-12-2011) General Provisions § 212.2 What is current good manufacturing practice...

  4. Immobilization of Organophosphorus Acid Anhydrolase Mutant Y212F on Silica Nanospheres

    Science.gov (United States)

    2016-09-01

    Y212F amino acid designations. 2 E. coli cell cultures were grown in Luria broth (Sigma-Aldrich Company; St. Louis, MO) with 100 µg/mL of...Express purification system (GE Healthcare Life Sciences; Uppsala, Sweden ) with a Q-sepharose column (HiPrep Q FF 16/10; GE Healthcare Life Sciences

  5. 25 CFR 212.42 - Annual rentals and expenditures for development on leases other than oil and gas, and geothermal...

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Annual rentals and expenditures for development on leases other than oil and gas, and geothermal resources. 212.42 Section 212.42 Indians BUREAU OF INDIAN AFFAIRS... other than oil and gas, and geothermal resources. The provisions of § 211.42 of this subchapter...

  6. 5 CFR 630.212 - Use of annual leave to establish initial eligibility for retirement or continuation of health...

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Use of annual leave to establish initial eligibility for retirement or continuation of health benefits. 630.212 Section 630.212 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS ABSENCE AND LEAVE Definitions and General...

  7. 41 CFR 301-71.212 - Should we report late payment fees as wages on a Form W-2?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false Should we report late payment fees as wages on a Form W-2? 301-71.212 Section 301-71.212 Public Contracts and Property... late payment fees as wages on a Form W-2? No, the Internal Revenue Service (IRS) has determined...

  8. 75 FR 55393 - Determination Under Section 1010(a) of the Supplemental Appropriations Act, 2010 (Pub. L. 111-212)

    Science.gov (United States)

    2010-09-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Determination Under Section 1010(a) of the Supplemental Appropriations Act, 2010 (Pub. L. 111-212) Pursuant to section 1010(a) of the Supplemental Appropriations Act, 2010 (Pub. L. 111-212) and the authority vested...

  9. 21 CFR 212.60 - What requirements apply to the laboratories where I test components, in-process materials, and...

    Science.gov (United States)

    2010-04-01

    ... I test components, in-process materials, and finished PET drug products? 212.60 Section 212.60 Food... of components, in-process materials, and finished PET drug products must have and follow written... identified to show the specific component, in-process material, or drug product for each lot tested. (4) A...

  10. 49 CFR 173.212 - Non-bulk packagings for solid hazardous materials in Packing Group II.

    Science.gov (United States)

    2010-10-01

    ... in Packing Group II. 173.212 Section 173.212 Transportation Other Regulations Relating to... materials in Packing Group II. (a) When § 172.101 of this subchapter specifies that a solid hazardous... of part 173, to the requirements of part 178 of this subchapter at the Packing Group I or...

  11. 18 CFR 2.12 - Calculation of taxes for property of public utilities and licensees constructed or acquired after...

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Calculation of taxes for property of public utilities and licensees constructed or acquired after January 1, 1970. 2.12 Section 2.12 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT...

  12. 20 CFR 652.212 - When should a State submit modifications to the five-year plan?

    Science.gov (United States)

    2010-04-01

    ... the five-year plan? 652.212 Section 652.212 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION... five-year plan? (a) A State may submit modifications to the five-year plan as necessary during the five... a viable and living document over its five-year life. (b) That portion of the plan addressing the...

  13. New cytogenetically visible copy number variant in region 8q21.2

    Directory of Open Access Journals (Sweden)

    Ewers Elisabeth

    2011-01-01

    Full Text Available Abstract Background Cytogenetically visible unbalanced chromosomal abnormalities (UBCA, reported for >50 euchromatic regions of almost all human autosomes, are comprised of a few megabases of DNA, and carriers are in many cases clinically healthy. It may be speculated, that some of the UBCA may be similar or identical to copy number variants (CNV of the human genome. Results Here we report on a yet unreported cytogenetically visible copy number variant (CNV in the long arm of chromosome 8, region 8q21.2, detected in three unrelated clinically healthy carriers. Conclusion The first description of a cytogenetically visible CNV/UBCA in 8q21.2 shows that banding cytogenetics is far from being outdated. It is a cost efficient, up-to-date method for a single cell specific overview on the whole genome, still prepared to deliver unexpected findings.

  14. Laboratory measurement of radioactivity purification for 212Pb in liquid scintillator

    Science.gov (United States)

    Hu, Wei; Fang, Jian; Yu, Bo-Xiang; Zhang, Xuan; Zhou, Li; Cai, Xiao; Sun, Li-Jun; Liu, Wan-Jin; Wang, Lan; Lü, Jun-Guang

    2016-09-01

    Liquid scintillator (LS) has been widely used in past and running neutrino experiments, and is expected also to be used in future experiments. Requirements on LS radio-purity have become higher and higher. Water extraction is a powerful method to remove soluble radioactive nuclei, and a mini-extraction station has been constructed. To evaluate the extraction efficiency and optimize the operation parameters, a setup to load radioactivity to LS and a laboratory scale setup to measure radioactivity using the 212Bi-212Po-208Pb cascade decay have been developed. Experience from this laboratory study will be useful for the design of large scale water extraction plants and the optimization of working conditions in the future. Supported by The Strategic Priority Research Program of the Chinese Academy of Sciences (XDA10010500), Natural Science Foundation of China (11390384)

  15. The effect of prolonged exposure to 750 C air on the tribological performance of PM212

    Science.gov (United States)

    Bemis, Kirk; Bogdanski, Michael S.; Dellacorte, Christopher; Sliney, Harold E.

    1994-01-01

    The effect of prolonged exposure to 750 C air on the tribological performance and dimensional stability of PM212, a high temperature, self-lubricating composite, is studied. PM212, by weight, contains 70 percent metal-bonded Cr3C2, 15 percent BaF2/CaF2 eutectic, and 15 percent silver. Rub blocks were fabricated from PM212 by cold isostatic pressing followed by sintering. Prior to tribo-testing, the rub blocks were exposed to 750 C air for periods ranging from 100 to 1000 hours. Then, the rub blocks were slid against nickel-based superalloy disks in a double-rub-block tribometer in air under a 66 N load at temperatures from 25 to 750 C with a sliding velocity of 0.36 m/s. Unexposed rub blocks were tested for baseline comparison. Friction coefficients ranged from 0.24 to 0.37 for the unexposed rub blocks and from 0.32 to 0.56 for the exposed ones. Wear for both the composite blocks and superalloy disks was typically in the moderate to low range of 10(exp -5) to 10(exp -6) mm(exp 3)/N-m. Friction and wear data were similar for the rub blocks exposed for 100, 500, and 1000 hours. Prolonged exposure to 750 C air increased friction and wear of the PM212 rub blocks at room temperature, but their triboperformance remained unaffected at higher temperatures, probably due to the formation of lubricious metal oxides. Dimensional stability of the composite was studied by exposing specimens of varying thicknesses for 500 hours in air at 750 C. Block thicknesses were found to increase with increased exposure time until steady state was reached after 100 hours of exposure, probably due to oxidation.

  16. ML212: A small-molecule probe for investigating fluconazole resistance mechanisms in Candida albicans

    Directory of Open Access Journals (Sweden)

    Willmen Youngsaye

    2013-07-01

    Full Text Available The National Institutes of Health Molecular Libraries and Probe Production Centers Network (NIH-MLPCN screened >300,000 compounds to evaluate their ability to restore fluconazole susceptibility in resistant Candida albicans isolates. Additional counter screens were incorporated to remove substances inherently toxic to either mammalian or fungal cells. A substituted indazole possessing the desired bioactivity profile was selected for further development, and initial investigation of structure–activity relationships led to the discovery of ML212.

  17. A Prototype {sup 212}Pb Medical Dose Calibrator for Alpha Radioimmunotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, W.F.; Patil, A.; Russ, W.R.; Newman, J. [CANBERRA Industries, Inc. (United States); Torgue, J. [AREVA Med (United States)

    2015-07-01

    AREVA Med, an AREVA group subsidiary, is developing innovative cancer-fighting therapies involving the use of {sup 212}Pb for alpha radioimmunotherapy. Canberra Industries, the nuclear measurement subsidiary of AREVA, has been working with AREVA Med to develop a prototype measurement system to assay syringes containing a {sup 212}Pb solution following production by an elution system. The relative fraction of emitted radiation from the source associated directly with the {sup 212}Pb remains dynamic for approximately 6 hours after the parent is chemically purified. A significant challenge for this measurement application is that the short half-life of the parent nuclide requires assay prior to reaching equilibrium with progeny nuclides. A gross counting detector was developed to minimize system costs and meet the large dynamic range of source activities. Prior to equilibrium, a gross counting system must include the period since the {sup 212}Pb was pure to calculate the count rate attributable to the parent rather than the progeny. The dynamic state is determined by solving the set of differential equations, or Bateman equations, describing the source decay behavior while also applying the component measurement efficiencies for each nuclide. The efficiencies were initially estimated using mathematical modeling (MCNP) but were then benchmarked with source measurements. The goal accuracy of the system was required to be within 5%. Independent measurements of the source using a high resolution spectroscopic detector have shown good agreement with the prototype system results. The prototype design was driven by cost, compactness and simplicity. The detector development costs were minimized by using existing electronics and firmware with a Geiger-Mueller tube derived from Canberra's EcoGamma environmental monitoring product. The acquisition electronics, communications and interface were controlled using Python with the EcoGamma software development kit on a Raspberry

  18. Hot methanol from the inner region of the HH 212 protostellar system

    CERN Document Server

    Leurini, S; Cabrit, S; Gueth, F; Giannetti, A; Bacciotti, F; Bachiller, R; Ceccarelli, C; Gusdorf, A; Lefloch, B; Podio, L; Tafalla, M

    2016-01-01

    The mechanisms leading to the formation of disks around young stellar objects (YSOs) and to the launching of the associated jets are crucial to the understanding of the earliest stages of star and planet formation. HH 212 is a privileged laboratory to study a pristine jet-disk system. Therefore we investigate the innermost region ($<100$ AU) around the HH 212-MM1 protostar through ALMA band\\,7 observations of methanol. The 8 GHz bandwidth spectrum towards the peak of the continuum emission of the HH 212 system reveals at least 19 transitions of methanol. Several of these lines (among which several vibrationally excited lines in the v$_{\\rm t}=1,2$ states) have upper energies above 500 K. They originate from a compact ($<135$ AU in diameter), hot ($\\sim 295$ K) region elongated along the direction of the SiO jet. We performed a fit in the $uv$ plane of various velocity channels of the strongest high-excitation lines. The blue- and red-shifted velocity centroids are shifted roughly symmetrically on either...

  19. Diagnosis and treatment of cystic neoplasms of pancreas: retrospective analysis of 212 cases%胰腺囊性肿瘤212例诊治分析

    Institute of Scientific and Technical Information of China (English)

    王单松; 靳大勇; 匡天涛; 许雪峰; 吴文川; 倪晓凌; 楼文晖

    2013-01-01

    目的 探讨胰腺囊性肿瘤诊断和治疗的原则及方法.方法 回顾性分析2001年1月至2010年12月复旦大学附属中山医院手术切除的212例胰腺囊性肿瘤病例的临床资料.结果 212例胰腺囊性肿瘤中,导管内乳头状黏液性肿瘤(IPMN) 101例(48%),黏液性囊性肿瘤(MCN)49例(23%),浆液性囊性肿瘤(SCN)62例(29%).病人无特征性临床表现;超声、CT和MRI对胰腺囊性肿瘤的诊断正确率分别达到90%,93%和94%;胰腺囊性肿瘤术后胰瘘发生率为34.3%;SCN和非侵袭性IPMN和MCN的5年存活率为100%,侵袭性IPMN和MCN的5年存活率分别为35%和31%.结论 CT及MRI是胰腺囊性肿瘤术前诊断的主要手段;SCN和非侵袭性IPMN和MCN预后好,功能保留性胰腺手术有应用价值,侵袭性IPMN和MCN应规则胰腺切除加区域淋巴结清扫;胰腺囊性肿瘤总体预后较好.

  20. WIN55,212-2 protects oligodendrocyte precursor cells in stroke penumbra following permanent focal cerebral ischemia in rats

    OpenAIRE

    SUN, JING; Fang, Yin-quan; Ren, Hong; Tao CHEN; Guo, Jing-Jing; Yan, Jun; SONG, SHU; Zhang, Lu-yong; Liao, Hong

    2012-01-01

    Aim: To explore whether the synthetic cannabinoid receptor agonist WIN55,212-2 could protect oligodendrocyte precursor cells (OPCs) in stroke penumbra, thereby providing neuroprotection following permanent focal cerebral ischemia in rats. Methods: Adult male SD rats were subjected to permanent middle cerebral artery occlusion (p-MCAO). The animals were administered WIN55,212-2 at 2 h, and sacrificed at 24 h after the ischemic insult. The infarct volumes and brain swelling were assessed. The e...

  1. Inhibition of interleukin-1β-induced endothelial tissue factor expression by the synthetic cannabinoid WIN 55,212-2.

    Science.gov (United States)

    Scholl, Antje; Ivanov, Igor; Hinz, Burkhard

    2016-09-20

    The role of cannabinoids in thrombosis remains controversial. In view of the primary importance of tissue factor (TF) in blood coagulation and its involvement in the pathology of several cardiovascular, inflammatory and neoplastic diseases, a regulation of this initial procoagulant signal seems to be of particular interest. Using human umbilical vein endothelial cells (HUVEC) the present study investigated the impact of the synthetic cannabinoid WIN 55,212-2 on interleukin (IL)-1β-induced TF expression and activity. WIN 55,212-2 caused a time- and concentration-dependent suppression of IL-1β-induced TF protein accompanied by decreases in TF mRNA and activity. Inhibition of TF protein expression by WIN 55,212-2 was mimicked by its cannabinoid receptor-inactive enantiomer WIN 55,212-3 but not by structurally unrelated phyto-, endo- and synthetic cannabinoids. In addition, the inhibitory effect of WIN 55,212-2 was not reversed by antagonists to cannabinoid receptors (CB1, CB2) or transient receptor potential vanilloid 1. Mechanistic approaches revealed WIN 55,212-2 to suppress IL-1β-induced TF expression via inhibition of ceramide formation and via decreased phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinases. Further inhibitor experiments demonstrated neutral sphingomyelinase (nSMase) to confer ceramide generation upon IL-1β treatment with the parallel IL-1β-mediated activation of MAPKs occurring via an nSMase-independent pathway. Finally, a receptor-independent inhibition of IL-1β-induced TF protein by WIN 55,212-2 was confirmed in human blood monocytes. Collectively, this data provide a hitherto unknown receptor-independent anticoagulatory action of the cannabinoid WIN 55,212-2.

  2. Histone demethylase retinoblastoma binding protein 2 is overexpressed in hepatocellular carcinoma and negatively regulated by hsa-miR-212.

    Directory of Open Access Journals (Sweden)

    Xiuming Liang

    Full Text Available BACKGROUND: The H3K4 demethylase retinoblastoma binding protein 2 (RBP2 is involved in the pathogenesis of gastric cancer, but its role and regulation in hepatocellular carcinoma (HCC is unknown. We determined the function of RBP2 and its regulation in HCC in vitro and in human tissues. METHODS: We analyzed gene expression in 20 specimens each of human HCC and normal liver tissue by quantitative real-time PCR and immunohistochemistry. Proliferation was analyzed by foci formation and senescence by β-galactosidase staining. Promoter activity was detected by luciferase reporter assay. RESULTS: The expression of RBP2 was stronger in cancerous than non-cancerous tissues, but that of its binding microRNA, Homo sapiens miR-212 (hsa-miR-212, showed an opposite pattern. SiRNA knockdown of RBP2 significantly upregulated cyclin-dependent kinase inhibitors (CDKIs, with suppression of HCC cell proliferation and induction of senescence. Overexpression of hsa-miR-212 suppressed RBP2 expression, with inhibited cell proliferation and induced cellular senescence, which coincided with upregulated CDKIs; with low hsa-miR-212 expression, CDKIs were downregulated in HCC tissue. Inhibition of hsa-miR-212 expression upregulated RBP2 expression. Luciferase reporter assay detected the direct binding of hsa-miR-212 to the RBP2 3' UTR. CONCLUSIONS: RBP2 is overexpressed in HCC and negatively regulated by hsa-miR-212. The hsa-miR-212-RBP2-CDKI pathway may be important in the pathogenesis of HCC.

  3. A novel temozolomide analog, NEO212, with enhanced activity against MGMT-positive melanoma in vitro and in vivo.

    Science.gov (United States)

    Chen, Thomas C; Cho, Hee-Yeon; Wang, Weijun; Nguyen, Jenny; Jhaveri, Niyati; Rosenstein-Sisson, Rachel; Hofman, Florence M; Schönthal, Axel H

    2015-03-28

    The alkylating agent temozolomide (TMZ) represents an important component of current melanoma therapy, but overexpression of O6-methyl-guanine DNA methyltransferase (MGMT) in tumor cells confers resistance to TMZ and impairs therapeutic outcome. We investigated a novel perillyl alcohol (POH)-conjugated analog of TMZ, NEO212, for its ability to exert anticancer activity against MGMT-positive melanoma cells. Human melanoma cells with variable MGMT expression levels were treated with NEO212, TMZ, or perillyl alcohol in vitro and in vivo, and markers of DNA damage and apoptosis, and tumor cell growth were investigated. NEO212 displayed substantially greater anticancer activity than any of the other treatments. It reduced colony formation of MGMT-positive cells up to eight times more effectively than TMZ, and much more potently induced DNA damage and cell death. In a nude mouse tumor model, NEO212 showed significant activity against MGMT-positive melanoma, whereas TMZ, or a mix of TMZ plus POH, was ineffective. At the same time, NEO212 was well tolerated. NEO212 may have potential as a more effective therapy for advanced melanoma, and should become particularly suitable for the treatment of patients with MGMT-positive tumors.

  4. (+)-WIN 55,212-2, a novel cannabinoid receptor agonist, exerts antidystonic effects in mutant dystonic hamsters.

    Science.gov (United States)

    Richter, A; Löscher, W

    1994-11-03

    The effects of the novel high affinity cannabinoid receptor agonist (+)-WIN 55,212-2 ((R)-4,5-dihydro-2-methyl-4(4-morphoinylmethyl)-1-(1-naphthalen ylcarbonyl)-6H-pyrrolo[3,2,1-ij]quinolin-6-one) on severity of dystonia were investigated in mutant Syrian hamsters with primary generalized dystonia. Following injections of (+)-WIN 55,212-2 (1.0-5.0 mg/kg i.p.) a dose-dependent reduction of the severity of dystonia was observed. At antidystonic doses (2.5 and 5.0 mg/kg i.p.) (+)-WIN 55,212-2 caused a reduction of spontaneous motor activity and catalepsy. 1 mg/kg of (+)-WIN 55,212-2 exhibited neither antidystonic effects nor any side effects. However, the coadministration of 1.0 mg/kg (+)-WIN 55,212-2 with an ineffective dose of diazepam (0.1 mg/kg i.p.) exerted antidystonic effects in the absence of severe side effects. Although psychotropic effects of cannabinoids, such as (+)-WIN 55,212-2, limit the therapeutical utility of cannabinoids, the present data indicate that cannabinoids exert antidystonic effects and that low doses of cannabinoids may increase antidystonic efficacy of benzodiazepines.

  5. SU-C-201-05: Imaging 212Pb-TCMC-Trastuzumab for Alpha Radioimmunotherapy for Ovarian Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Shen, S; Meredith, R; Azure, M; Yoder, D [University of Alabama, Birmingham, AL (United States); Torgue, J; Banaga, E [AREVA Med LLC, Bethesda, MD (United States)

    2015-06-15

    Purpose: To support the phase I trial for toxicity, biodistribution and pharmacokinetics of intra-peritoneal (IP) 212Pb-TCMC-trastuzumab in patients with HER-2 expressing malignancy. A whole body gamma camera imaging method was developed for estimating amount of 212Pb-TCMC-trastuzumab left in the peritoneal cavity. Methods: {sup 212}Pb decays to {sup 212}Bi via beta emission. {sup 212}Bi emits an alpha particle at an average of 6.1 MeV. The 238.6 keV gamma ray with a 43.6% yield can be exploited for imaging. Initial phantom was made of saline bags with 212Pb. Images were collected for 238.6 keV with a medium energy general purpose collimator. There are other high energy gamma emissions (e.g. 511keV, 8%; 583 keV, 31%) that penetrate the septae of the collimator and contribute scatter into 238.6 keV. An upper scatter window was used for scatter correction for these high energy gammas. Results: A small source containing 212Pb can be easily visualized. Scatter correction on images of a small 212Pb source resulted in a ∼50% reduction in the full width at tenth maximum (FWTM), while change in full width at half maximum (FWHM) was <10%. For photopeak images, substantial scatter around phantom source extended to > 5 cm outside; scatter correction improved image contrast by removing this scatter around the sources. Patient imaging, in the 1st cohort (n=3) showed little redistribution of 212Pb-TCMC-trastuzumab out of the peritoneal cavity. Compared to the early post-treatment images, the 18-hour post-injection images illustrated the shift to more uniform anterior/posterior abdominal distribution and the loss of intensity due to radioactive decay. Conclusion: Use of medium energy collimator, 15% width of 238.6 keV photopeak, and a 7.5% upper scatter window is adequate for quantification of 212Pb radioactivity inside peritoneal cavity for alpha radioimmunotherapy of ovarian cancer. Research Support: AREVA Med, NIH 1UL1RR025777-01.

  6. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong-Kook [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Henry, Jon C. [Department of Surgery, Ohio State University, Columbus, OH 43210 (United States); Jiang, Jinmai [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States); Esau, Christine [Regulus Therapeutics, Carlsbad, CA (United States); Gusev, Yuriy [Lombardi Cancer Center, Georgetown University, Washington, DC (United States); Lerner, Megan R. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Postier, Russell G. [Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States); Brackett, Daniel J. [Veterans Affairs Medical Center, Oklahoma City, OK (United States); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH 43210 (United States)

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  7. Lightcurve photometry of asteroids 212 Medea, 517 Edith, 3581 Alvarez 5682 Beresford, and 5817 Robertfrazer

    Science.gov (United States)

    Koff, Robert A.

    2005-06-01

    Lightcurve period and amplitude results are reported from Antelope Hills Observatory: 212 Medea 10.283±0.001 hr and 0.08±0.01 mag.; 517 Edith 9.274±0.001 hr and 0.12±0.01 mag.; 3581 Alvarez 33.42±0.02 hr, and 0.06±0.02 mag.; 5682 Beresford 7.536±0.002 hr and 0.20±0.02 mag.; 5817 Robertfrazer 4.051±0.001 hr and 0.33±0.02 mag.

  8. Gene expression profiling upon (212) Pb-TCMC-trastuzumab treatment in the LS-174T i.p. xenograft model.

    Science.gov (United States)

    Yong, Kwon J; Milenic, Diane E; Baidoo, Kwamena E; Kim, Young-Seung; Brechbiel, Martin W

    2013-10-01

    Recent studies have demonstrated that therapy with (212) Pb-TCMC-trastuzumab resulted in (1) induction of apoptosis, (2) G2/M arrest, and (3) blockage of double-strand DNA damage repair in LS-174T i.p. (intraperitoneal) xenografts. To further understand the molecular basis of the cell killing efficacy of (212) Pb-TCMC-trastuzumab, gene expression profiling was performed with LS-174T xenografts 24 h after exposure to (212) Pb-TCMC-trastuzumab. DNA damage response genes (84) were screened using a quantitative real-time polymerase chain reaction array (qRT-PCR array). Differentially regulated genes were identified following exposure to (212) Pb-TCMC-trastuzumab. These included genes involved in apoptosis (ABL, GADD45α, GADD45γ, PCBP4, and p73), cell cycle (ATM, DDIT3, GADD45α, GTSE1, MKK6, PCBP4, and SESN1), and damaged DNA binding (DDB) and repair (ATM and BTG2). The stressful growth arrest conditions provoked by (212) Pb-TCMC-trastuzumab were found to induce genes involved in apoptosis and cell cycle arrest in the G2/M phase. The expression of genes involved in DDB and single-strand DNA breaks was also enhanced by (212) Pb-TCMC-trastuzumab while no modulation of genes involved in double-strand break repair was apparent. Furthermore, the p73/GADD45 signaling pathway mediated by p38 kinase signaling may be involved in the cellular response, as evidenced by the enhanced expression of genes and proteins of this pathway. These results further support the previously described cell killing mechanism by (212) Pb-TCMC-trastuzumab in the same LS-174T i.p. xenograft. Insight into these mechanisms could lead to improved strategies for rational application of radioimmunotherapy using α-particle emitters.

  9. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor.

    Science.gov (United States)

    Park, Jong-Kook; Henry, Jon C; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R; Postier, Russell G; Brackett, Daniel J; Schmittgen, Thomas D

    2011-03-25

    Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G(2)/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the β2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The β2 adrenergic pathway may play an important role in this novel mechanism. Copyright © 2011 Elsevier Inc. All rights reserved.

  10. The tribology of PS212 coatings and PM212 composites for the lubrication of titanium 6A1-4V components of a Stirling engine space power system

    Science.gov (United States)

    Sliney, Harold E.; Dellacorte, Christopher; Lukaszewicz, Victor

    1995-01-01

    The Stirling space power machine incorporates a linear alternator to generate electrical power. The alternator is a reciprocating device that is driven by a solar or nuclear-powered Stirling engine. The power piston and cylinder are made of titanium 6A1-4V (Ti6-4) alloy, and are designed to be lubricated by a hydrodynamically-generated gas film. Rubbing occurs during starts and stops and there is a possibility of an occasional high speed rub. Since titanium is known to have a severe galling tendency in sliding contacts, a 'backup,' self-lubricating coating on the cylinder and/or the piston is needed. This report describes the results of a research program to study the lubrication of Ti6-4 with the following chromium carbide based materials: plasma-sprayed PS212 coatings and sintered PM212 counterfaces. Program objectives are to achieve adherent coatings on Ti6-4 and to measure the friction and wear characteristics of the following sliding combinations under conditions simulative of the Stirling-driven space power linear alternator: Ti6-4/Ti6-4 baseline, Ti6-4/PS212 coated Ti6-4, and Ps212 coated Ti6-4/PM212

  11. The tribology of PS212 coatings and PM212 composites for the lubrication of titanium 6A1-4V components of a Stirling engine space power system

    Science.gov (United States)

    Sliney, Harold E.; Dellacorte, Christopher; Lukaszewicz, Victor

    1995-01-01

    The Stirling space power machine incorporates a linear alternator to generate electrical power. The alternator is a reciprocating device that is driven by a solar or nuclear-powered Stirling engine. The power piston and cylinder are made of titanium 6A1-4V (Ti6-4) alloy, and are designed to be lubricated by a hydrodynamically-generated gas film. Rubbing occurs during starts and stops and there is a possibility of an occasional high speed rub. Since titanium is known to have a severe galling tendency in sliding contacts, a 'backup,' self-lubricating coating on the cylinder and/or the piston is needed. This report describes the results of a research program to study the lubrication of Ti6-4 with the following chromium carbide based materials: plasma-sprayed PS212 coatings and sintered PM212 counterfaces. Program objectives are to achieve adherent coatings on Ti6-4 and to measure the friction and wear characteristics of the following sliding combinations under conditions simulative of the Stirling-driven space power linear alternator: Ti6-4/Ti6-4 baseline, Ti6-4/PS212 coated Ti6-4, and Ps212 coated Ti6-4/PM212

  12. Water and acetaldehyde in HH212: The first hot corino in Orion

    CERN Document Server

    Codella, C; Cabrit, S; Podio, L; Bachiller, R; Fontani, F; Gusdorf, A; Lefloch, B; Leurini, S; Tafalla, M

    2016-01-01

    Aims: Using the unprecedented combination of high resolution and sensitivity offered by ALMA, we aim to investigate whether and how hot corinos, circumstellar disks, and ejected gas are related in young solar-mass protostars. Methods: We observed CH$_3$CHO and deuterated water (HDO) high-excitation ($E_{\\rm u}$ up to 335 K) lines towards the Sun-like protostar HH212--MM1. Results: For the first time, we have obtained images of CH$_3$CHO and HDO emission in the inner $\\simeq$ 100 AU of HH212. The multifrequency line analysis allows us to contrain the density ($\\geq$ 10$^{7}$ cm$^{-3}$), temperature ($\\simeq$ 100 K), and CH$_3$CHO abundance ($\\simeq$ 0.2--2 $\\times$ 10$^{-9}$) of the emitting region. The HDO profile is asymmetric at low velocities ($\\leq$ 2 km s$^{-1}$ from $V_{\\rm sys}$). If the HDO line is optically thick, this points to an extremely small ($\\sim$ 20--40 AU) and dense ($\\ge$ 10$^{9}$ cm$^{-3}$) emitting region. Conclusions: We report the first detection of a hot corino in Orion. The HDO asymm...

  13. An evaluation of the membership probability of 212 $\\lambda$ Boo stars: I. A Catalogue

    CERN Document Server

    Murphy, Simon J; Gray, Richard O; Cheng, Kwang-Ping; Neff, James E; Koen, Chris; Kuehn, Charles A; Newsome, Ian; Riggs, Quinlin

    2015-01-01

    The literature on the $\\lambda$ Boo stars has grown to become somewhat heterogenous, as different authors have applied different criteria across the UV, optical and infrared regions to determine the membership status of $\\lambda$ Boo candidates. We aim to clear up the confusion by consulting the literature on 212 objects that have been considered as $\\lambda$ Boo candidates, and subsequently evaluating the evidence in favour of their admission to the $\\lambda$ Boo class. We obtained new spectra of $\\sim$90 of these candidates and classified them on the MK system to aid in the membership evaluations. The re-evaluation of the 212 objects resulted in 64 members and 103 non-members of the $\\lambda$ Boo class, with a further 45 stars for which membership status is unclear. We suggest observations for each of the stars in the latter category that will allow them to be confidently included or rejected from the class. Our reclassification facilitates homogenous analysis on group members, and represents the largest co...

  14. Isotope shifts and hyperfine structure of {sup 212-214}Ra{sup +}

    Energy Technology Data Exchange (ETDEWEB)

    Giri, Gouri S.; Sahoo, Bijaya K.; Versolato, Oscar O.; Wansbeek, Lotje W.; Willmann, Lorenz; Wilschut, Hans W.; Jungmann, Klaus; Timmermans, Rob G.E. [Kernfysisch Versneller Instituut, University of Groningen (Netherlands)

    2010-07-01

    The availability of short lived Ra isotopes at the TRI{mu}P facility of the KVI makes the study of atomic structure of these isotopes possible. {sup 212-214}Ra ions are trapped in a buffer-gas filled Paul trap. Isotope shifts and hyperfine structures of {sup 212-214}Ra{sup +} for several transitions were obtained by laser spectroscopy. Ra{sup +} is of particular interest for a most precise measurement of Atomic Parity Violation (APV), currently underway in our group. Such a measurement will provide a stringent test of the Standard Model of particle physics. However, the interpretation of APV measurements require the determination of the weak matrix elements to better than 1% accuracy in order to extract the weak charge (Weinberg angle). Currently, our calculations are accurate to some 3%. We therefore plan to exploit the fact that radium has a wide range of isotopes by looking at the ratio E1APV/ E1'APV for two -or more- isotopes, and thus canceling atomic uncertainties. Here, nuclear structure effects start to play a role. The extracted isotope shifts and hyperfine interactions provide indispensable information. In addition, the measured hyperfine structure of {sup 213}Ra{sup +} is of particular interest for the proposed Radium single ion clock.

  15. Real-time video compression system design and hardware implementation based on multiple ADV212

    Institute of Scientific and Technical Information of China (English)

    徐冬冬

    2016-01-01

    In order to improve the transmission rate of the compression system , a real-time video lossy compression system based on multiple ADV 212 is proposed and achieved .Considering the CMOS video format and the working principle of ADV 212 , a Custom-specific mode is used for various video formats firstly .The data can be cached through the FPGA internal RAM and SDRAM Ping-Pong op-eration.And the working efficiency is greatly promoted .Secondly, this method can realize direct code stream transmission or do it after storage .Through the error correcting coding , the correction ability of the flash memory is highly improved .Lastly, the compression and de-compression circuit boards are involved to specify the performance of the method .The results show that the compression system has a real-time and stable performance .And the compression ratio can be changed arbitrarily by configuring the program .The compression system can be realized and the real-time performance is good with large amount of data .

  16. The Structure of manganese oxide formed by the fungus Acremonium sp. strain KR21-2

    Science.gov (United States)

    Saratovsky, Ian; Gurr, Sarah J.; Hayward, Michael A.

    2009-06-01

    Manganese oxides are observed to form by the oxidation of aqueous solutions of Mn(II) catalyzed by the action of microorganisms. In contrast to the widely studied material produced by bacteria, manganese oxide phases produced by the action of fungi have received only limited attention. A detailed study of the MnO x material produced by the action of the fungus Acremonium KR21-2, utilizing X-ray diffraction, XANES, EXAFS and transmission electron microscopy is reported. The MnO x material is produced as small crystalline particles which adopt a todorokite-like tunnel structure, in striking contrast to previously reported microbial MnO x materials which adopt layered birnessite-type structures. ICPMS measurements reveal there are no templating metal ions present in the fungally mediated MnO x material, in contrast to analogous bacterially mediated material, suggesting these cations play a critical role in determining the structure of the material precipitated. A phylogenetic analysis places KR21-2 with other Acremonium species in the Hypocreales.

  17. miR-212/132 expression and functions: within and beyond the neuronal compartment

    Science.gov (United States)

    Wanet, Anaïs; Tacheny, Aurélie; Arnould, Thierry; Renard, Patricia

    2012-01-01

    During the last two decades, microRNAs (miRNAs) emerged as critical regulators of gene expression. By modulating the expression of numerous target mRNAs mainly at the post-transcriptional level, these small non-coding RNAs have been involved in most, if not all, biological processes as well as in the pathogenesis of a number of diseases. miR-132 and miR-212 are tandem miRNAs whose expression is necessary for the proper development, maturation and function of neurons and whose deregulation is associated with several neurological disorders, such as Alzheimer's disease and tauopathies (neurodegenerative diseases resulting from the pathological aggregation of tau protein in the human brain). Although their involvement in neuronal functions is the most described, evidences point towards a role of these miRNAs in many other biological processes, including inflammation and immune functions. Incidentally, miR-132 was recently classified as a ‘neurimmiR’, a class of miRNAs operating within and between the neural and immune compartments. In this review, we propose an outline of the current knowledge about miR-132 and miR-212 functions in neurons and immune cells, by describing the signalling pathways and transcription factors regulating their expression as well as their putative or demonstrated roles and validated mRNA targets. PMID:22362752

  18. Opioid pain medication use after dermatologic surgery: a prospective observational study of 212 dermatologic surgery patients.

    Science.gov (United States)

    Harris, KaLynne; Curtis, Julia; Larsen, Brooke; Calder, Scott; Duffy, Keith; Bowen, Glen; Hadley, Michael; Tristani-Firouzi, Payam

    2013-03-01

    To better understand postoperative opioid use after dermatologic surgery. Prospective observational study. Academic dermatology department. The study included 212 adults (1) who were undergoing a single skin excision (including Mohs micrographic surgery), (2) who consented to participate,and (3) who were able to be reached by telephone on postoperative day 3 or 4. Patients who did not meet these criteria and those referred to another physician for further surgical treatment or repair were excluded. The study examined(1) the incidence of opioid prescription after dermatologic surgery, (2) the percentage of prescribed opioid pain medications used in the postoperative period, and (3) patient and surgical characteristics associated with opioid pain medication prescription and use. Opioids were prescribed to 72 of the 212 patients(34%). Twenty-five of the 72 patients (35%) who were prescribed opioids did not use them. Forty-nine of 57 patients (86%) who filled an opioid prescription had leftover pills, and 26 of the 49 patients (53%) planned to keep them. Only maximum pain score was significantly associated with opioid use. Opioids were over prescribed after dermatologic surgery. Patients who had left over opioids did not dispose of them properly, which could lead to potential misuse and abuse.

  19. Multiepoch Radio Observations of the Exciting Sources of HH 212 and HH 119

    Directory of Open Access Journals (Sweden)

    R. Galván Madrid

    2004-01-01

    Full Text Available Presentamos observaciones de alta resolución angular (- 0.``3 hechas con el Very Large Array a 3.5 cm hacia las regiones de las fuentes excitadoras de dos sistemas Herbig-Haro: HH 212 en Orión y HH 119 en B335. Las observaciones fueron hechas durante tres épocas diferentes con el objetivo de medir posibles variaciones temporales rápidas (en la escala de una semana a un mes en las fuentes. En HH 212 detectamos, promediando las observaciones de las tres épocas, una fuente muy débil justo en la posición esperada para la fuente excitadora del sistema HH. La comparación con otras observaciones de radio de esta fuente indica variación temporal considerable en escala de años. En B335 detectamos en cada época al objeto previamente reportado por otros autores y nuestros resultados sugieren que esta fuente, un chorro térmico de radio, es variable en escalas de tiempo de un mes. Discutimos posibles explicaciones para esta rápida variabilidad.

  20. Cellular uptake of {sup 212}BiOCl by Ehrlich ascites cells: A dosimetric analysis

    Energy Technology Data Exchange (ETDEWEB)

    Roeske, J.C.; Whitlock, J.L.; Harper, P.V.; Rotmensch, J. [Univ. of Chicago, IL (United States); Stinchcomb, T.G. [DePaul Univ., Chicago, IL (United States). Dept. of Physics; Schwartz, J.L. [Univ. of Washington, Seattle, WA (United States). Dept. of Radiation Oncology; Hines, J.J. [Argonne National Lab., IL (United States). Chemistry Div.

    1999-01-01

    Bi-212 is an alpha-emitting radionuclide being investigated as a therapeutic agent in the intraperitoneal treatment of micrometastatic ovarian carcinoma. In evaluating a new therapeutic modality, cell-survival studies are often used as a means of quantifying the biological effects of radiation. In this analysis, Ehrlich ascites cells were irradiated under conditions similar to therapy in various concentrations of Bi-212. Immediately following irradiation, a cell survival assay was performed in which cells were plated and colonies were counted after 10--14 days. Both a macrodosimetric and a microdosimetric approach were used in analyzing these data. These models used as input the fraction of activity within the cell and in solution, the distribution of cell sizes, and the variation of LET along individual alpha-particle tracks. The results indicate that the energy deposited within the nucleus varies significantly among individual cells. There is a small fraction of cell nuclei which receive no hits, while the remaining cells receive energy depositions which can differ significantly from the mean value. These dosimetric parameters are correlated with measured cell survival and will be a useful predictor of outcome for therapeutic doses.

  1. EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS

    Directory of Open Access Journals (Sweden)

    E. G. Lobanova

    2009-01-01

    Full Text Available Abstract. Ability of cannabinoid receptor ligands WIN 55.212-2 and anandamid to inhibit synthesis of TNFα and IL-8 was studied in healthy donors and men with allergic disorders. To establish mechanism of action for investigated substances, the selective antagonists of the СВ1-receptor (SR141716A and for СВ2 - receptor (SR144528 were applied. Studies with whole blood dilutions allowed of approximating in vivo conditions when investigating biological properties of WIN-55.212-2 and anandamid. The synthetic cannabinoids WIN - 55.212-2 and anandamid at a concentration of 3-10 μМ were capable of reducing synthesis of TNFα and IL-8 in lipopolysaccharide-stimulated blood leukocytes, both from healthy donors and subjects with allergic disorders. It was revealed that the antagonist of СВ1-receptor (SR141716A did not exert a receptor-mediated effect for WIN-55.212-2 and anandamid. Meanwhile, a СВ2-receptor antagonist (SR144528 entirely eliminated completely the blocking effect of anandamid and WIN-55.212-2.

  2. Antibodies against analogous heptad repeat peptide HR212 of Newcastle Disease Virus inhibit virus-cell membrane fusion

    Institute of Scientific and Technical Information of China (English)

    LI Ying; TIEN Po

    2007-01-01

    Membrane fusion is a key step in enveloped virus entry. Highly conserved heptad repeat regions (HR1 and HR2) of Newcastle disease virus (NDV) fusion protein (F) are critical functional domains for viral membrane fusion. They display different conformations in the membrane fusion states and are viewed as candidate targets for neutralizing antibody responses. We previously reported that an analog of heptad repeat peptides HR2-HR1-HR2(HR212) and HR2 could inhibit NDV induced cell-cell membrane fusion. Here, we show that HR212 can induce the production of highly potent antibody in immunized rabbits, which could recognize full length peptides of both HR1 and HR2, and inhibit NDV hemagglutination and NDV entry. These suggest that either HR212 or its antibody could be an inhibitor of virus-induced cell-cell membrane fusion.

  3. Mechanical strength and thermophysical properties of PM212: A high temperature self-lubricating powder metallurgy composite

    Science.gov (United States)

    Edwards, Phillip M.; Sliney, Harold E.; Dellacorte, Christopher; Whittenberger, J. Daniel; Martineau, Robert R.

    1990-01-01

    A powder metallurgy composite, PM212, composed of metal bonded chromium carbide and solid lubricants is shown to be self-lubricating to a maximum application temperature of 900 C. The high temperature compressive strength, tensile strength, thermal expansion and thermal conductivity data needed to design PM212 sliding contact bearings and seals are reported for sintered and isostatically pressed (HIPed) versions of PM212. Other properties presented are room temperature density, hardness, and elastic modulus. In general, both versions appear to have adequate strength to be considered as sliding contact bearing materials, but the HIPed version, which is fully dense, is much stronger than the sintered version which contains about 20 percent pore volume. The sintered material is less costly to make, but the HIPed version is better where high compressive strength is important.

  4. Evaluation of WIN 55,212-2 self-administration in rats as a potential cannabinoid abuse liability model

    Science.gov (United States)

    Lefever, Timothy W.; Marusich, Julie A.; Antonazzo, Kateland R.; Wiley, Jenny L.

    2014-01-01

    Because Δ9-tetrahydrocannabinol (THC) has been a false negative in rat intravenous self-administration procedures, evaluation of the abuse potential of candidate cannabinoid medications has proved difficult. One lab group has successfully trained self-administration of the aminoalkylindole WIN55,212-2 in rats; however, their results have not been independently replicated. The purpose of this study was to extend their model by using a within-subjects design, with the goal of establishing a robust method suitable for substitution testing of other cannabinoids. Male Long-Evans rats were trained to self-administer WIN55,212-2 (0.01 mg/kg/infusion) on a fixed ratio 3 schedule. Dose-effect curves for WIN55,212-2 were determined, followed by vehicle substitution and a dose-effect curve with THC. WIN55,212-2 self-administration was acquired; however, substitution with THC did not maintain responding above vehicle levels. Dose-dependent attenuation by rimonabant confirmed CB1 receptor mediation of WIN55,212-2’s reinforcing effects. Vehicle substitution resulted in a session-dependent decrease in responding (i.e., extinction). While this study provides systematic replication of previous studies, lack of substitution with THC is problematic and suggests that WIN55,212-2 self-administration may be of limited usefulness as a screening tool for detection of the reinforcing effects of potential cannabinoid medications. Clarification of underlying factors responsible for failure of THC to maintain self-administration in cannabinoid-trained rats is needed. PMID:24412835

  5. WIN55,212-2 protects oligodendrocyte precursor cells in stroke penumbra following permanent focal cerebral ischemia in rats

    Institute of Scientific and Technical Information of China (English)

    Jing SUN; Yin-quan FANG; Hong REN; Tao CHEN; Jing-jing GUO; Jun YAN; Shu SONG; Lu-yong ZHANG; Hong LIAO

    2013-01-01

    Aim:To explore whether the synthetic cannabinoid receptor agonist WIN55,212-2 could protect oligodendrocyte precursor cells (OPCs)in stroke penumbra,thereby providing neuroprotection following permanent focal cerebral ischemia in rats.Methods:Adult male SD rats were subjected to permanent middle cerebral artery occlusion (p-MCAO).The animals were administered WIN55,212-2 at 2 h,and sacrificed at 24 h after the ischemic insult.The infarct volumes and brain swelling were assessed.The expression of cannabinoid receptor type 1 (CB1) in the stroke penumbra was examined using Western blot assay.The pathological changes and proliferation of neural glial antigen 2-positive OPCs (NG2+ cells) in the stroke penumbra were studied using immunohistochemistry staining.Results:p-MCAO significantly increased the expression of CB1 within the stroke penumbra with the highest level appearing at 2 h following the ischemic insult.Administration of WIN55,212-2 (9 mg/kg,iv) significantly attenuated the brain swelling,and reduced the infarct volume as well as the number of tau-immunoreactive NG2+ cells (tau-1+/NG2+ cells) in the stroke penumbra.Moreover,WIN55,212-2 significantly promoted the proliferation of NG2+ cells in the stroke penumbra and in the ipsilateral subventricular zone at 24 h following the ischemic insult.Administration of the selective CB1 antagonist rimonabant (1 mg/kg,iv) partially blocked the effects caused by WIN55,212-2.Conclusion:Tau-1 is expressed in NG2+ cells following permanent focal cerebral ischemic injury.Treatment with WIN55,212-2 reduces the number of tau-1+/NG2+ cells and promotes NG2+ cell proliferation in the stroke penumbra,which are mediated partially via CB1 and may contribute to its neuroprotective effects.

  6. Sodium, Saturated Fat, and Sugar Added Intake of The Diet of Children 2-12 Years Old

    OpenAIRE

    Angga Hardiansyah; Hardinsyah Hardinsyah; Dadang Sukandar

    2015-01-01

    The aims of the study were to analyze intake of sodium, saturated fat (SFA), and added sugar of children 2-12 years old. For this purpose, 38.890 children 2-12 years old from the food consumption data of the basic health survey of the Ministry of Health were analyzed. The intake of sodium, SFA, and added sugar were calculated by using food composition table (FCT) of Indonesia and USDA, and from nutrition facts of labeled foods.The sodium and SFA calculated include both natural resources and w...

  7. El Decreto reglamentario de la Ley N° 18.212 (Usura: su ilegitimidad

    Directory of Open Access Journals (Sweden)

    Roque Molla

    2010-07-01

    Full Text Available En nuestra entrega para el Tomo XXXIX del Anuario de Derecho Civil Uruguay, ratificamos nuestra opinión vertida en La Pluma No. 29 Junio 2008, en el sentido de que el régimen de la ley 18.212 no es aplicable a la cláusula penal pactada en una compraventa para el caso de resolución del contrato por incumplimiento de la obligación de pagar el precio.La cuestión debe resolverse en función de la materia regulada por la ley.En ese orden de ideas, en el trabajo publicado en el ADCU referido, siguiendo a Emilio Betti, manifestamos: "La materia (ratio legis de la ley es la disciplina de la usura civil y penal abarcativa no solamente de los intereses usurarios sino también de los negocios usurarios en las operaciones de crédito"

  8. X-ray absorption toward the red quasar 3C 212

    Science.gov (United States)

    Elvis, Martin; Fiore, Fabrizio; Mathur, Smita; Wilkes, Belinda J.

    1994-04-01

    A Roentgen Satellite (ROSAT) X-ray spectrum of the z = 1.049 'red quasar' 3C 212 has a strong low-energy cutoff. The spectrum can be fitted with a power law (of energy index 1.4(+0.8, -0.6) with low-energy photoelectric absorption in excess of the Galactic value that, if at the redhsift of the quasar, would have a column density of (0.9(+0.8, -0.6)) x 1022 atoms/sq cm. Possible sites for the absorption are a nuclear torus, an intervening damped Lyman-alpha system, or intracluster material (e.g., a cooling flow) around the quasar. The implied absorbing column density is sufficient to redden a normal quasar spectrum to the observed steep optical slope. The observed continuum, if dereddened by this amount, can produce the observed emission line fluxes and ratios. The absence of the graphite lambda-2175 feature in 3C 212 however, requires dust different from the local Milky Way composition, or an intervening absorber with z less than 0.4. Alternative acceptable fits to the X-ray spectrum are (1) a blackbody with a temperature of 0.7 keV (in the quasar frame) modified only by Galactic absorption, and (2) an optically thin thermal plasma with excess absorption. Although a blackbody spectrum would be unprecedented, the model is consistent with all the available X-ray and optical data and cannot be ruled out. We discuss possible observations that can discriminate among the above models.

  9. 76 FR 2130 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2011-01-12

    ... exercise of authority creates no substantive or procedural right or benefit that is legally enforceable by... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and... benefit or protection under the INA and has been determined to be otherwise eligible for the benefit...

  10. 76 FR 2131 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2011-01-12

    ... regarding any subsequent benefit or protection applications, unless such exercise of authority has been... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and...) Is seeking a benefit or protection under the INA and has been determined to be otherwise eligible...

  11. Polymorphisms in a Putative Enhancer at the 10q21.2 Breast Cancer Risk Locus Regulate NRBF2 Expression

    NARCIS (Netherlands)

    H. Darabi (Hatef); K. McCue (Karen); J. Beesley (Jonathan); K. Michailidou (Kyriaki); S. Nord (Silje); S. Kar (Siddhartha); K. Humphreys (Keith); D. Thompson (Deborah); M. Ghoussaini (Maya); M.K. Bolla (Manjeet); J. Dennis (Joe); Q. Wang (Qing); S. Canisius (Sander); C.G. Scott (Christopher G.); C. Apicella (Carmel); J. Hopper (John); M.C. Southey (Melissa); J. Stone (Jennifer); A. Broeks (Annegien); M.K. Schmidt (Marjanka); R.J. Scott (Rodney J.); A. Lophatananon (Artitaya); K.R. Muir (K.); M.W. Beckmann (Matthias W.); A.B. Ekici (Arif); P.A. Fasching (Peter); K. Heusinger (Katharina); I. dos Santos Silva (Isabel); J. Peto (Julian); I.P. Tomlinson (Ian); E.J. Sawyer (Elinor); B. Burwinkel (Barbara); F. Marme (Federick); P. Guénel (Pascal); T. Truong (Thérèse); S.E. Bojesen (Stig); H. Flyger (Henrik); J. Benítez (Javier); A. González-Neira (Anna); H. Anton-Culver (Hoda); S.L. Neuhausen (Susan); V. Arndt (Volker); H. Brenner (Hermann); C. Engel (Christoph); A. Meindl (Alfons); R.K. Schmutzler (Rita); N. Arnold (Norbert); H. Brauch (Hiltrud); U. Hamann (Ute); J. Chang-Claude (Jenny); S. Khan (Sofia); H. Nevanlinna (Heli); H. Ito (Hidemi); K. Matsuo (Keitaro); N.V. Bogdanova (Natalia); T. Dörk (Thilo); A. Lindblom (Annika); S. Margolin (Sara); V-M. Kosma (Veli-Matti); A. Mannermaa (Arto); C.-C. Tseng (Chiu-chen); A.H. Wu (Anna H.); O.A.M. Floris; D. Lambrechts (Diether); A. Rudolph (Anja); P. Peterlongo (Paolo); P. Radice (Paolo); F.J. Couch (Fergus); C. Vachon (Celine); G.G. Giles (Graham G.); C.A. McLean (Catriona Ann); R.L. Milne (Roger L.); P.-A. Dugué (Pierre-Antoine); C.A. Haiman (Christopher); G. Maskarinec (Gertraud); C. Woolcott (Christy); B.E. Henderson (Brian); M.S. Goldberg (Mark); J. Simard (Jacques); S.-H. Teo; S. Mariapun (Shivaani); Å. Helland (Åslaug); V. Haakensen (Vilde); W. Zheng (Wei); A. Beeghly-Fadiel (Alicia); R. Tamimi (Rulla); A. Jukkola-Vuorinen (Arja); R. Winqvist (Robert); I.L. Andrulis (Irene); J.A. Knight (Julia); P. Devilee (Peter); R.A.E.M. Tollenaar (Rob); J.D. Figueroa (Jonine); M. García-Closas (Montserrat); K. Czene (Kamila); M.J. Hooning (Maartje); M.M.A. Tilanus-Linthorst (Madeleine); J. Li (J.); Y.-T. Gao (Yu-Tang); X.-O. Shu (Xiao-Ou); A. Cox (Angela); S.S. Cross (Simon); R.N. Luben (Robert); K.T. Khaw; J.-Y. Choi (J.); D. Kang (Daehee); J.M. Hartman (Joost); W.-Y. Lim (Wei-Yen); M. Kabisch (Maria); D. Torres (Diana); A. Jakubowska (Anna); J. Lubinski (Jan); J.D. McKay (James); S. Sangrajrang (Suleeporn); A.E. Toland (Amanda); D. Yannoukakos (Drakoulis); C-Y. Shen (Chen-Yang); J-C. Yu (Jyh-Cherng); A. Ziogas (Argyrios); M. Schoemaker (Minouk); A.J. Swerdlow (Anthony ); A.-L. Borresen-Dale (Anne-Lise); V. Kristensen (Vessela); J.D. French (Juliet); S.L. Edwards (Stacey); A.M. Dunning (Alison); D.F. Easton (Douglas); P. Hall (Per); G. Chenevix-Trench (Georgia)

    2015-01-01

    textabstractGenome-wide association studies have identified SNPs near ZNF365 at 10q21.2 that are associated with both breast cancer risk and mammographic density. To identify the most likely causal SNPs, we fine mapped the association signal by genotyping 428 SNPs across the region in 89,050 Europea

  12. 48 CFR 212.570 - Applicability of certain laws to contracts and subcontracts for the acquisition of commercially...

    Science.gov (United States)

    2010-10-01

    ... laws to contracts and subcontracts for the acquisition of commercially available off-the-shelf items..., DEPARTMENT OF DEFENSE ACQUISITION PLANNING ACQUISITION OF COMMERCIAL ITEMS Applicability of Certain Laws to the Acquisition of Commercial Items 212.570 Applicability of certain laws to contracts...

  13. 40 CFR 262.212 - Making the hazardous waste determination at an on-site interim status or permitted treatment...

    Science.gov (United States)

    2010-07-01

    ....212 Making the hazardous waste determination at an on-site interim status or permitted treatment... hazardous waste permit or interim status as soon as it arrives in the on-site treatment, storage or disposal... permitted treatment, storage or disposal facility. (e) If the unwanted material is a hazardous waste, the...

  14. 76 FR 14419 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2011-03-16

    ... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and...(d)(3)(B)(i) of the Immigration and Nationality Act (INA), 8 U.S.C. 1182(d)(3)(B)(i), as amended, as.... Citizenship and Immigration Services (USCIS), in consultation with U.S. Immigration and Customs...

  15. 76 FR 14418 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2011-03-16

    ... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and...(d)(3)(B)(i) of the Immigration and Nationality Act (INA), 8 U.S.C. 1182(d)(3)(B)(i), as amended, as... determination will be made by U.S. Citizenship and Immigration Services (USCIS), in consultation with...

  16. 77 FR 41795 - Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and Nationality Act

    Science.gov (United States)

    2012-07-16

    ... SECURITY Office of the Secretary Exercise of Authority Under Section 212(d)(3)(B)(i) of the Immigration and...(d)(3)(B)(i) of the Immigration and ] Nationality Act (INA), 8 U.S.C. 1182(d)(3)(B)(i), as amended.... Implementation of this determination will be made by U.S. Citizenship and Immigration Services (USCIS),...

  17. 49 CFR 180.212 - Repair of seamless DOT 3-series specification cylinders and seamless UN pressure receptacles.

    Science.gov (United States)

    2010-10-01

    ... TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification, Maintenance and Use of Cylinders § 180.212 Repair of seamless DOT 3-series specification... pressure receptacle unless— (i) The repair facility holds an approval issued under the provisions in §...

  18. 20 CFR 645.212 - Who may be served under the general eligibility and noncustodial parent eligibility (primary...

    Science.gov (United States)

    2010-04-01

    ...-TO-WORK GRANTS General Program and Administrative Requirements § 645.212 Who may be served under the... TANF program, and/or its predecessor program, for at least 30 months, although the months do not have... consultation with local boards and WtW competitive grantees, or (iii) “Having difficulty paying child...

  19. Polymorphisms in a Putative Enhancer at the 10q21.2 Breast Cancer Risk Locus Regulate NRBF2 Expression

    NARCIS (Netherlands)

    H. Darabi (Hatef); K. McCue (Karen); J. Beesley (Jonathan); K. Michailidou (Kyriaki); S. Nord (Silje); S. Kar (Siddhartha); K. Humphreys (Keith); D. Thompson (Deborah); M. Ghoussaini (Maya); M.K. Bolla (Manjeet); J. Dennis (Joe); Q. Wang (Qing); S. Canisius (Sander); C.G. Scott (Christopher G.); C. Apicella (Carmel); J. Hopper (John); M.C. Southey (Melissa); J. Stone (Jennifer); A. Broeks (Annegien); M.K. Schmidt (Marjanka); R.J. Scott (Rodney J.); A. Lophatananon (Artitaya); K.R. Muir (K.); M.W. Beckmann (Matthias W.); A.B. Ekici (Arif); P.A. Fasching (Peter); K. Heusinger (Katharina); I. dos Santos Silva (Isabel); J. Peto (Julian); I.P. Tomlinson (Ian); E.J. Sawyer (Elinor); B. Burwinkel (Barbara); F. Marme (Federick); P. Guénel (Pascal); T. Truong (Thérèse); S.E. Bojesen (Stig); H. Flyger (Henrik); J. Benítez (Javier); A. González-Neira (Anna); H. Anton-Culver (Hoda); S.L. Neuhausen (Susan); V. Arndt (Volker); H. Brenner (Hermann); C. Engel (Christoph); A. Meindl (Alfons); R.K. Schmutzler (Rita); N. Arnold (Norbert); H. Brauch (Hiltrud); U. Hamann (Ute); J. Chang-Claude (Jenny); S. Khan (Sofia); H. Nevanlinna (Heli); H. Ito (Hidemi); K. Matsuo (Keitaro); N.V. Bogdanova (Natalia); T. Dörk (Thilo); A. Lindblom (Annika); S. Margolin (Sara); V-M. Kosma (Veli-Matti); A. Mannermaa (Arto); C.-C. Tseng (Chiu-chen); A.H. Wu (Anna H.); O.A.M. Floris; D. Lambrechts (Diether); A. Rudolph (Anja); P. Peterlongo (Paolo); P. Radice (Paolo); F.J. Couch (Fergus); C. Vachon (Celine); G.G. Giles (Graham G.); C.A. McLean (Catriona Ann); R.L. Milne (Roger L.); P.-A. Dugué (Pierre-Antoine); C.A. Haiman (Christopher); G. Maskarinec (Gertraud); C. Woolcott (Christy); B.E. Henderson (Brian); M.S. Goldberg (Mark); J. Simard (Jacques); S.-H. Teo; S. Mariapun (Shivaani); Å. Helland (Åslaug); V. Haakensen (Vilde); W. Zheng (Wei); A. Beeghly-Fadiel (Alicia); R. Tamimi (Rulla); A. Jukkola-Vuorinen (Arja); R. Winqvist (Robert); I.L. Andrulis (Irene); J.A. Knight (Julia); P. Devilee (Peter); R.A.E.M. Tollenaar (Rob); J.D. Figueroa (Jonine); M. García-Closas (Montserrat); K. Czene (Kamila); M.J. Hooning (Maartje); M.M.A. Tilanus-Linthorst (Madeleine); J. Li (J.); Y.-T. Gao (Yu-Tang); X.-O. Shu (Xiao-Ou); A. Cox (Angela); S.S. Cross (Simon); R.N. Luben (Robert); K.T. Khaw; J.-Y. Choi (J.); D. Kang (Daehee); J.M. Hartman (Joost); W.-Y. Lim (Wei-Yen); M. Kabisch (Maria); D. Torres (Diana); A. Jakubowska (Anna); J. Lubinski (Jan); J.D. McKay (James); S. Sangrajrang (Suleeporn); A.E. Toland (Amanda); D. Yannoukakos (Drakoulis); C-Y. Shen (Chen-Yang); J-C. Yu (Jyh-Cherng); A. Ziogas (Argyrios); M. Schoemaker (Minouk); A.J. Swerdlow (Anthony ); A.-L. Borresen-Dale (Anne-Lise); V. Kristensen (Vessela); J.D. French (Juliet); S.L. Edwards (Stacey); A.M. Dunning (Alison); D.F. Easton (Douglas); P. Hall (Per); G. Chenevix-Trench (Georgia)

    2015-01-01

    textabstractGenome-wide association studies have identified SNPs near ZNF365 at 10q21.2 that are associated with both breast cancer risk and mammographic density. To identify the most likely causal SNPs, we fine mapped the association signal by genotyping 428 SNPs across the region in 89,050

  20. 8 CFR 212.14 - Parole determinations for alien witnesses and informants for whom a law enforcement authority...

    Science.gov (United States)

    2010-01-01

    ... and informants for whom a law enforcement authority (âLEAâ) will request S classification. 212.14... determinations for alien witnesses and informants for whom a law enforcement authority (“LEA”) will request S...) needed for law enforcement purposes provided that a state or federal LEA: (i) Establishes its intention...

  1. 36 CFR 212.8 - Permission to cross lands and easements owned by the United States and administered by the Forest...

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Permission to cross lands and easements owned by the United States and administered by the Forest Service. 212.8 Section 212.8 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TRAVEL MANAGEMENT Administration of...

  2. microRNA-132/212 deficiency enhances Aβ production and senile plaque deposition in Alzheimer's disease triple transgenic mice.

    Science.gov (United States)

    Hernandez-Rapp, Julia; Rainone, Sara; Goupil, Claudia; Dorval, Véronique; Smith, Pascal Y; Saint-Pierre, Martine; Vallée, Maxime; Planel, Emmanuel; Droit, Arnaud; Calon, Frédéric; Cicchetti, Francesca; Hébert, Sébastien S

    2016-08-03

    The abnormal regulation of amyloid-β (Aβ) metabolism (e.g., production, cleavage, clearance) plays a central role in Alzheimer's disease (AD). Among endogenous factors believed to participate in AD progression are the small regulatory non-coding microRNAs (miRs). In particular, the miR-132/212 cluster is severely reduced in the AD brain. In previous studies we have shown that miR-132/212 deficiency in mice leads to impaired memory and enhanced Tau pathology as seen in AD patients. Here we demonstrate that the genetic deletion of miR-132/212 promotes Aβ production and amyloid (senile) plaque formation in triple transgenic AD (3xTg-AD) mice. Using RNA-Seq and bioinformatics, we identified genes of the miR-132/212 network with documented roles in the regulation of Aβ metabolism, including Tau, Mapk, and Sirt1. Consistent with these findings, we show that the modulation of miR-132, or its target Sirt1, can directly regulate Aβ production in cells. Finally, both miR-132 and Sirt1 levels correlated with Aβ load in humans. Overall, our results support the hypothesis that the miR-132/212 network, including Sirt1 and likely other target genes, contributes to abnormal Aβ metabolism and senile plaque deposition in AD. This study strengthens the importance of miR-dependent networks in neurodegenerative disorders, and opens the door to multifactorial drug targets of AD by targeting Aβ and Tau.

  3. A highly-collimated SiO jet in the HH212 protostellar outflow

    CERN Document Server

    Codella, C; Gueth, F; Cesaroni, R; Bacciotti, F; Le Floc'h, B; McCaughrean, M J

    2006-01-01

    We mapped the HH212 Class 0 outflow in SiO(2--1, 5--4) and continuum using the PdBI in its extended configurations. The unprecedented angular resolution (down to 0.34") allows accurate comparison with a new, deep H2 image obtained at the VLT. The SiO emission is confined to a highly-collimated bipolar jet (width 0.35") along the outflow axis. The jet can be traced down to within 500 AU of the protostar, in a region that is heavily obscured in H2 images. Where both species are detected, SiO shows the same overall kinematics and structure as H2, indicating that both molecules are tracing the same material. We find that the high-velocity SiO gas near the protostar is not tracing a wide-angle wind but is already confined to a flow inside a narrow cone of half-opening angle < 6 deg.

  4. Hot methanol from the inner region of the HH 212 protostellar system

    Science.gov (United States)

    Leurini, S.; Codella, C.; Cabrit, S.; Gueth, F.; Giannetti, A.; Bacciotti, F.; Bachiller, R.; Ceccarelli, C.; Gusdorf, A.; Lefloch, B.; Podio, L.; Tafalla, M.

    2016-10-01

    The mechanisms leading to the formation of disks around young stellar objects (YSOs) and to the launching of the associated jets are crucial to the understanding of the earliest stages of star and planet formation. HH 212 is a privileged laboratory to study a pristine jet-disk system. Therefore we investigate the innermost region (uv plane of various velocity channels of the strongest high-excitation lines. The blue- and red-shifted velocity centroids are shifted roughly symmetrically on either side of the jet axis, indicating that the line-of-sight velocity beyond 0.7 km s-1 from systemic is dominated by rotational motions. The velocity increases moving away from the protostar further indicating that the emission of methanol is not associated with a Keplerian disk or rotating-infalling cavity, and it is more likely associated with outflowing gas. We speculate that CH3OH traces a disk wind gas accelerated at the base. The launching region would be at a radius of a few astronomical units from the YSO.

  5. A 140 GHz pulsed EPR/212 MHz NMR spectrometer for DNP studies

    Science.gov (United States)

    Smith, Albert A.; Corzilius, Björn; Bryant, Jeffrey A.; DeRocher, Ronald; Woskov, Paul P.; Temkin, Richard J.; Griffin, Robert G.

    2012-10-01

    We described a versatile spectrometer designed for the study of dynamic nuclear polarization (DNP) at low temperatures and high fields. The instrument functions both as an NMR spectrometer operating at 212 MHz (1H frequency) with DNP capabilities, and as a pulsed-EPR operating at 140 GHz. A coiled TE011 resonator acts as both an NMR coil and microwave resonator, and a double balanced (1H, 13C) radio frequency circuit greatly stabilizes the NMR performance. A new 140 GHz microwave bridge has also been developed, which utilizes a four-phase network and ELDOR channel at 8.75 GHz, that is then multiplied and mixed to obtain 140 GHz microwave pulses with an output power of 120 mW. Nutation frequencies obtained are as follows: 6 MHz on S = 1/2 electron spins, 100 kHz on 1H, and 50 kHz on 13C. We demonstrate basic EPR, ELDOR, ENDOR, and DNP experiments here. Our solid effect DNP results demonstrate an enhancement of 144 and sensitivity gain of 310 using OX063 trityl at 80 K and an enhancement of 157 and maximum sensitivity gain of 234 using Gd-DOTA at 20 K, which is significantly better performance than previously reported at high fields (⩾3 T).

  6. Kinematics of SiO J=8-7 Emission towards the HH 212 Jet

    CERN Document Server

    Takami, M; Momose, M; Hayashi, M; Davis, C; Pyo, T S; Nishikawa, T; Kohno, K; Takami, Michihiro; Takamuwa, Shigehisa; Momose, Munetake; Hayashi, Masa; Davis, Christopher; Pyo, Tae-Soo; Nishikawa, Takayuki; Kohno, Kotaro

    2006-01-01

    We present SiO J=8-7 (347.3 GHz) observations towards HH 212 using the ASTE telescope. Our observations with a 22''-diameter beam show that the SiO emission is highly concentrated within 1' of the driving source. We carefully compare the SiO observations with archival H_2 1-0 S(1) images and published H_2 echelle spectra. We find that, although the SiO velocities closely match the radial velocities seen in H_2, the distribution of H_2 and SiO emission differ markedly. We attribute the latter to the different excitation conditions required for H_2 and SiO emission, particularly the higher critical density (n_H2 ~10^8 cm^-3) of the SiO J=8-7 emission. The kinematic similarities imply that the H_2 and SiO are associated with the same internal working surfaces. We conclude that the SiO J=8-7 emission has a potential to probe the jet/wind launching region through interferometric observations in the future, particularly for the youngest, most deeply embedded protostars where IR observations are not possible.

  7. A 140 GHz pulsed EPR/212 MHz NMR spectrometer for DNP studies.

    Science.gov (United States)

    Smith, Albert A; Corzilius, Björn; Bryant, Jeffrey A; DeRocher, Ronald; Woskov, Paul P; Temkin, Richard J; Griffin, Robert G

    2012-10-01

    We described a versatile spectrometer designed for the study of dynamic nuclear polarization (DNP) at low temperatures and high fields. The instrument functions both as an NMR spectrometer operating at 212 MHz ((1)H frequency) with DNP capabilities, and as a pulsed-EPR operating at 140 GHz. A coiled TE(011) resonator acts as both an NMR coil and microwave resonator, and a double balanced ((1)H, (13)C) radio frequency circuit greatly stabilizes the NMR performance. A new 140 GHz microwave bridge has also been developed, which utilizes a four-phase network and ELDOR channel at 8.75 GHz, that is then multiplied and mixed to obtain 140 GHz microwave pulses with an output power of 120 mW. Nutation frequencies obtained are as follows: 6 MHz on S=1/2 electron spins, 100 kHz on (1)H, and 50 kHz on (13)C. We demonstrate basic EPR, ELDOR, ENDOR, and DNP experiments here. Our solid effect DNP results demonstrate an enhancement of 144 and sensitivity gain of 310 using OX063 trityl at 80 K and an enhancement of 157 and maximum sensitivity gain of 234 using Gd-DOTA at 20 K, which is significantly better performance than previously reported at high fields (≥3 T).

  8. Sistemas Correctores de Campo Para EL Telescopio Ritchey-Chretien UNAM212

    Science.gov (United States)

    Cobos, F. J.; Galan, M. J.

    1987-05-01

    El telescopio UNAM2l2 fue inaugurado hace siete años y concebido para trabajar en las razones focales: f/7.5, F/13.5, F/27 y F/98. El diseño Ritchey-Chretién corresponde a la razón focal F/7.5 y el foco primario (F/2.286) no se consideró como utilizable para fotografía directa. En el Instituto de Astronomía de la UNAM, se diseñó y construyó un sistema corrector de campo para la razón focal F/7.5, que actualmente está en funcionamiento. Dentro de un programa de colaboración en diseflo y evaluación de sistemas ópticos, entre el Instituto de Astrofísica de Canarias y el Instituto de Astronomía de la UNAM, decidimos intentar el diseño de una correctora de campo para el foco primario del tȩlescopio UNAM212 bajo la consideración de que no son insalvables los problemas que implicaría su instalación y de que es muy posible que, en un futuro relativamente cercano, podamom tener un detector bidimenmional tipo Mepsicrón cuya área sensible haga tentadora la idea de construir la cámara directa para foco primario

  9. Production and optimization of curdlan produced by Pseudomonas sp. QL212.

    Science.gov (United States)

    Yang, Min; Zhu, Ying; Li, Yumei; Bao, Jie; Fan, Xiangyu; Qu, Yanhong; Wang, Yiwei; Hu, Zhiheng; Li, Qiang

    2016-08-01

    Curdlan is a polysaccharide that consists of β-1,3-linked glucose residues. A polysaccharide-producing bacterium isolated from soil samples was identified as Pseudomonas sp. QL212. The polysaccharide was purified to homogeneity via sequential ethanol precipitation, deproteinization, CM ion-exchange, and gel chromatography sequentially. Analysis of the purified polysaccharide revealed that it consisted of many glucosyl residues, and its molecular weight was only 6.18×10(5)Da. This low molecular weight endowed it with excellent solubility. Infrared and nuclear magnetic resonance spectral analysis confirmed that the polysaccharide was curdlan. Single-factor and Response surface methodology experiments were used to optimize the culture medium and conditions. The optimal culture conditions consisted of seed culture age of 12h, and an incubation temperature of 30°C, with 10% inoculum and a total fluid volume of 75mL in a 250-mL Erlenmeyer flask. The maximum curdlan yield of about 5.92gL(-1) was achieved with an optimal medium consisting of 30.11gL(-1) of sucrose, 5.94gL(-1) of yeast extract, and an initial pH of 8.03. To our best knowledge, this is the highest reported yield of curdlan produced by Pseudomonas sp., and the curdlan production medium components were much simpler than those in previous reports.

  10. A potential regulatory loop between Lin28B:miR‑212 in androgen-independent prostate cancer.

    Science.gov (United States)

    Borrego-Diaz, Emma; Powers, Benjamin C; Azizov, Vugar; Lovell, Scott; Reyes, Ruben; Chapman, Bradley; Tawfik, Ossama; McGregor, Douglas; Diaz, Francisco J; Wang, Xinkun; Veldhuizen, Peter Van

    2014-12-01

    Lin28 is a family of RNA binding proteins and microRNA regulators. Two members of this family have been identified: Lin28A and Lin28B, which are encoded by genes localized in different chromosomes but share a high degree of sequence identity. The role of Lin28B in androgen-independent prostate cancer (AIPC) is not well understood. Lin28B is expressed in all grades of prostatic carcinomas and prostate cancer cell lines, but not in normal prostate tissue. In this study we found that Lin28B co-localized in the nucleus and cytoplasm of the DU145 AIPC. The expression of Lin28B protein positively correlated with the expression of the c-Myc protein in the prostate cancer cell lines and silencing of Lin28B also correlated with a lower expression of the c-Myc protein, but not with the downregulation of c-Myc messenger RNA (mRNA) in the DU145 AIPC cells. We hypothesized that Lin28B regul-ates the expression of c-Myc protein by altering intermediate c-Myc suppressors. Therefore, a microRNA profile of DU145 cells was performed after Lin28B siRNA silencing. Nineteen microRNAs were upregulated and eleven microRNAs were downregulated. The most upregulated microRNAs were miR-212 and miR-2278. Prior reports have found that miR-212 is suppressed in prostate cancer. We then ran TargetScan software to find potential target mRNAs of miR-212 and miR-2278, and it predicted Lin28B mRNA as a potential target of miR-212, but not miR-2278. TargetScan also predicted that c-Myc mRNA is not a potential target of miR-212 or miR-2278. These observations suggest that Lin28B:miR-212 may work as a regulatory loop in androgen-independent prostate cancer. Furthermore, we report a predictive 2-fold symmetric model generated by the superposition of the Lin28A structure onto the I-TASSER model of Lin28B. This structural model of Lin28B suggests that it shows unique microRNA binding characteristics. Thus, if Lin28B were to bind miRNAs in a manner similar to Lin28A, conformational changes would be

  11. A potential regulatory loop between Lin28B:miR-212 in androgen-independent prostate cancer

    Science.gov (United States)

    BORREGO-DIAZ, EMMA; POWERS, BENJAMIN C.; AZIZOV, VUGAR; LOVELL, SCOTT; REYES, RUBEN; CHAPMAN, BRADLEY; TAWFIK, OSSAMA; McGREGOR, DOUGLAS; DIAZ, FRANCISCO J.; WANG, XINKUN; VAN VELDHUIZEN, PETER

    2014-01-01

    Lin28 is a family of RNA binding proteins and microRNA regulators. Two members of this family have been identified: Lin28A and Lin28B, which are encoded by genes localized in different chromosomes but share a high degree of sequence identity. The role of Lin28B in androgen-independent prostate cancer (AIPC) is not well understood. Lin28B is expressed in all grades of prostatic carcinomas and prostate cancer cell lines, but not in normal prostate tissue. In this study we found that Lin28B co-localized in the nucleus and cytoplasm of the DU145 AIPC. The expression of Lin28B protein positively correlated with the expression of the c-Myc protein in the prostate cancer cell lines and silencing of Lin28B also correlated with a lower expression of the c-Myc protein, but not with the downregulation of c-Myc messenger RNA (mRNA) in the DU145 AIPC cells. We hypothesized that Lin28B regulates the expression of c-Myc protein by altering intermediate c-Myc suppressors. Therefore, a microRNA profile of DU145 cells was performed after Lin28B siRNA silencing. Nineteen microRNAs were upregulated and eleven microRNAs were downregulated. The most upregulated microRNAs were miR-212 and miR-2278. Prior reports have found that miR-212 is suppressed in prostate cancer. We then ran TargetScan software to find potential target mRNAs of miR-212 and miR-2278, and it predicted Lin28B mRNA as a potential target of miR-212, but not miR-2278. TargetScan also predicted that c-Myc mRNA is not a potential target of miR-212 or miR-2278. These observations suggest that Lin28B:miR-212 may work as a regulatory loop in androgen-independent prostate cancer. Furthermore, we report a predictive 2-fold symmetric model generated by the superposition of the Lin28A structure onto the I-TASSER model of Lin28B. This structural model of Lin28B suggests that it shows unique microRNA binding characteristics. Thus, if Lin28B were to bind miRNAs in a manner similar to Lin28A, conformational changes would be necessary

  12. 基于多片ADV212芯片的图像压缩系统设计%Design of Image Compression System Based on Multiple ADV212 Chip

    Institute of Scientific and Technical Information of China (English)

    于巍巍; 马晓东; 孙娟; 郝修强

    2014-01-01

    At present,the technology of static image compression coder based on JPEG2000 standard is the occupied technology in satellite communications. The ADV212 chip is a special ASIC which can realize integrally JPEG2000 image compression. To reasonably schedule multiple ADV212 chips to realize jointly teamwork,a new image compression system is proposed and implemented in this paper. For large-scale image in high speed,this system can implement the lossy compres-sion or the lossless compression in real-time. Thus a coding method of high-speed large-capacity satellite image compression is established.%基于JPEG2000标准的静态图像压缩编码器技术是当前应用于卫星通信中的热点技术。 ADV212芯片是一款可以完整实现JPEG2000图像压缩的专用ASIC。文章介绍了一种通过合理调度多片ADV212芯片来实现联合工作的图像压缩系统的设计方法和实现过程,使得系统对高输入数据率的宽幅图像可以进行实时无损或者有损压缩,从而提供了一种高速率大容量卫星图像压缩编码方法。

  13. Application of 212Pb for Targeted α-particle Therapy (TAT: Pre-clinical and Mechanistic Understanding through to Clinical Translation

    Directory of Open Access Journals (Sweden)

    Kwon Yong

    2015-08-01

    Full Text Available Targeted α-particle therapy (TAT, in which an α-particle emitting radionuclide is specifically directed to a biological target, is gaining more attention to treat cancers as new targets are validated. Bio-vectors such as monoclonal antibodies are able to selectively transport α-particles to destroy targeted cancer cells. TAT has the potential for an improved therapeutic ratio over β-particle targeted conjugate therapy. The short path length and the intense ionization path generated render α-emitters suitable for treatment and management of minimal disease such as micrometastases or residual tumor after surgical debulking. 212Pb is the longer-lived parent radionuclide of 212Bi and serves as an in vivo generator of 212Bi. 212Pb has demonstrated significant utility in both in vitro and in vivo models. Recent evaluation of 212Pb-TCMC-trastuzumab in a Phase I clinical trial has demonstrated the feasibility of 212Pb in TAT for the treatment of ovarian cancer patients. This review highlights progress in radionuclide production, radiolabeling chemistry, molecular mechanisms, and application of 212Pb to targeted pre-clinical and clinical radiation therapy for the management and treatment of cancer.

  14. Rice OsRAD21-2 is Expressed in Actively Dividing Tissues and its Ectopic Expression in Yeast Results in Aberrant Cell Division and Growth

    Institute of Scientific and Technical Information of China (English)

    Chunyan Gong; Tang Li; Qi Li; Longfeng Yan; Tai Wang

    2011-01-01

    Rad21 and its meiotic counterpart Rec8,the key components of the cohesin complex,are essential for sister chromatid cohesion and chromosome segregation in mitosis and meiosis,respectively.In contrast to yeast and vertebrates,which have only two RAD21/REC8 genes,the rice genome encodes four Rad21/Rec8 proteins.Here,we report on the cloning and characterization of OsRAD21-2 from rice (Oryza sativa L.).Phylogenetic analysis of the full-length amino acids showed that OsRad21-2 was grouped into the plant-specific Rad21 subfamily.Semi-quantitative reverse transcription-polymerase chain reaction revealed OsRAD21-2 preferentially expressed in premeiotic flowers.Further RNA in situ hybridization analysis and promoter::β-glucuronidase staining indicated that OsRAD21-2 was mainly expressed in actively dividing tissues including premeiotic stamen,stem intercalary meristem,leaf meristem,and root pericycle.Ectopic expression of OsRAD21-2 in fission yeast resulted in cell growth delay and morphological abnormality.Flow cytometric analysis revealed that the OsRAD21-2-expressed cells were arrested in G2 phase.Our results suggest that OsRad21-2 functions in regulation of cell division and growth.

  15. miR-132/212 knockout mice reveal roles for these miRNAs in regulating cortical synaptic transmission and plasticity.

    Directory of Open Access Journals (Sweden)

    Judit Remenyi

    Full Text Available miR-132 and miR-212 are two closely related miRNAs encoded in the same intron of a small non-coding gene, which have been suggested to play roles in both immune and neuronal function. We describe here the generation and initial characterisation of a miR-132/212 double knockout mouse. These mice were viable and fertile with no overt adverse phenotype. Analysis of innate immune responses, including TLR-induced cytokine production and IFNβ induction in response to viral infection of primary fibroblasts did not reveal any phenotype in the knockouts. In contrast, the loss of miR-132 and miR-212, while not overtly affecting neuronal morphology, did affect synaptic function. In both hippocampal and neocortical slices miR-132/212 knockout reduced basal synaptic transmission, without affecting paired-pulse facilitation. Hippocampal long-term potentiation (LTP induced by tetanic stimulation was not affected by miR-132/212 deletion, whilst theta burst LTP was enhanced. In contrast, neocortical theta burst-induced LTP was inhibited by loss of miR-132/212. Together these results indicate that miR-132 and/or miR-212 play a significant role in synaptic function, possibly by regulating the number of postsynaptic AMPA receptors under basal conditions and during activity-dependent synaptic plasticity.

  16. Drug Survival and reasons for discontinuation of intramuscular methotrexate: a study of 212 consecutive patients switching from oral methotrexate

    DEFF Research Database (Denmark)

    Linde, Louise; Hetland, Merete Lund; Østergaard, Mikkel

    2006-01-01

    Drug survival and reasons for discontinuation of intramuscular methotrexate: a study of 212 consecutive patients switching from oral methotrexate.Linde L, Hetland ML, Ostergaard M. Department of Rheumatology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark. louiselinde......@dadlnet.dk OBJECTIVES: To assess the drug survival and reasons for discontinuation of intramuscular methotrexate (imMTX) in rheumatological patients who had switched to imMTX from oral methotrexate (oMTX). METHODS: Data from 212 consecutive patients who switched from oMTX to imMTX therapy at our outpatient clinic...... had stopped oMTX because of adverse events, 22% also withdrew from imMTX because of adverse events. CONCLUSION: Half of the patients benefited from switching from oral to intramuscular methotrexate for at least 6 months, but only a minority adhered to the treatment for years. Lack of efficacy...

  17. Quantum yield of Cl∗ (21/2) production in the gas phase photolysis of CCl4 in the ultraviolet

    Indian Academy of Sciences (India)

    Manish Tak; Manabendra Chandra; Dulal Senapati; Puspendu K Das

    2006-07-01

    In this paper, we have probed the dynamics of chlorine atom production from the gas phase photodissociation of carbon tetrachloride at 222 and 235 nm. The quantum yield, * of Cl∗ (21/2) production has been determined by probing the nascent concentrations of both excited (21/2) and ground state (23/2) chlorine atoms by suitable resonance-enhanced multiphoton ionization (REMPI) detection schemes. Although at the photolysis wavelengths the absorption of carbon tetrachloride is weak, significant amounts of Cl∗ are produced. Surprisingly, the quantum yield of Cl∗ production does not follow the absorption spectrum closely, which gives rise to the possibility of an indirect dissociation mechanism present in CCl4 along with direct dissociation at these ultraviolet wavelengths.

  18. The microRNA-132/212 family fine-tunes multiple targets in Angiotensin II signalling in cardiac fibroblasts

    DEFF Research Database (Denmark)

    Eskildsen, Tilde V; Schneider, Mikael; Sandberg, Maria B;

    2015-01-01

    , signalling molecules and transcription factors. Subsequent comprehensive in silico analysis identified 24 target genes, of which 22 genes were qPCR validated. We identified seven genes involved in AngII signalling pathways. CONCLUSION: We here report novel insight of an extensive network of molecular......INTRODUCTION: MicroRNAs (miRNAs) are emerging as key regulators of cardiovascular development and disease; however, the cardiac miRNA target molecules are not well understood. We and others have described the Angiotensin II (AngII)-induced miR-132/212 family as novel regulators of cardiovascular...... function including regulation of cardiac hypertrophy, heart failure and blood pressure possibly through AT1R signalling. However, the miR-132/212 targets in the heart remain unknown. MATERIALS AND METHODS: To understand the role of these miRNAs in cardiac signalling networks, we undertook comprehensive...

  19. 21.2% wall-plug efficiency green laser based on an electrically pumped VECSEL through intracavity second harmonic generation

    Science.gov (United States)

    Zhao, Pu; Xu, Bing; van Leeuwen, Robert; Chen, Tong; Watkins, Laurence; Zhou, Delai; Seurin, Jean-Francois; Gao, Peng; Xu, Guoyang; Wang, Qing; Ghosh, Chuni

    2015-03-01

    We have achieved a 21.2% wall-plug efficiency green laser at 532 nm based on an electrically pumped vertical externalcavity surface emitting laser (VECSEL) through intracavity second harmonic generation. The continuous-wave green output power was 3.34 W. The VECSEL gain device is cooled by using a thermoelectric cooler, which can greatly benefit packaging. Both power and efficiency can be further scaled up by optimizing external-cavity design and improving the performance of VECSEL gain device.

  20. Carbon isotope ratios suggest no additional methane from boreal wetlands during the rapid Greenland Interstadial 21.2

    Science.gov (United States)

    Sperlich, Peter; Schaefer, Hinrich; Mikaloff Fletcher, Sara; Guillevic, Myriam; Lassey, Keith; Sapart, Célia; Röckmann, Thomas; Blunier, Thomas

    2017-04-01

    Samples from two Greenland ice cores (NEEM and NGRIP) have been measured for methane carbon isotope ratios (delta 13C-CH4) to investigate the CH4 mixing ratio anomaly during Greenland Interstadial (GI) 21.2 (85,000 years before present). This extraordinarily rapid event occurred within 150 years, comprising a CH4 mixing ratio pulse of 150 ppb (˜25%). Our new measurements disclose a concomitant shift in delta 13C-CH4 of 1‰ . Keeling plot analyses reveal the delta 13C of the additional CH4 source constituting the CH4 anomaly as -56.8 ± 2.8‰ , which we confirm by means of a previously published box model. We propose tropical wetlands as the most probable additional CH4 source during GI-21.2 and present independent evidence that suggests that tropical wetlands in South America and Asia have played a key role. We find no evidence that boreal CH4 sources, such as permafrost degradation, contributed significantly to the atmospheric CH4 increase, despite the pronounced warming in the Northern Hemisphere during GI-21.2.

  1. A new nonsyndromic X-linked sensorineural hearing impairment linked to Xp21.2

    Energy Technology Data Exchange (ETDEWEB)

    Lalwani, A.K.; Brister, J.R.; Fex, J.; Grundfast, K.M.; Pikus, A.T.; Ploplis, B.; San Agustin, T.; Skarka, H.; Wilcox, E.R. [National Institutes of Health, Bethesda, MD (United States)

    1994-10-01

    X-linked deafness is a rare cause of hereditary hearing impairment. We have identified a family with X-linked dominant sensorineural hearing impairment, characterized by incomplete penetrance and variable expressivity in carrier females, that is linked to the Xp21.2, which contains the Duchenne muscular dystrophy (DMD) locus. The auditory impairment in affected males was congenital, bilateral, profound, sensorineural, affecting all frequencies, and without evidence of radiographic abnormality of the temporal bone. Adult carrier females manifested bilateral, mild-to-moderate high-frequency sensorineural hearing impairment of delayed onset during adulthood. Eighteen commercially available polymorphic markers from the X chromosome, generating a 10-15-cM map, were initially used for identification of a candidate region. DXS997, located within the DMD gene, generated a two-point LOD score of 2.91 at {theta} = 0, with every carrier mother heterozygous at this locus. Recombination events at DXS992 (located within the DMD locus, 3{prime} to exon 50 of the dystrophin gene) and at DXS1068 (5{prime} to the brain promoter of the dystrophin gene) were observed. No recombination events were noted with the following markers within the DMD locus: 5{prime}DYS II, intron 44, DXS997, and intron 50. There was no clinical evidence of Duchenne or Becker muscular dystrophy in any family member. It is likely that this family represents a new locus on the X chromosome, which when mutated results in nonsyndromic sensorineural hearing loss and is distinct from the heterogeneous group of X-linked hearing losses that have been previously described. 57 refs., 6 figs., 1 tab.

  2. The new version 2.12 of BKG Ntrip Client (BNC)

    Science.gov (United States)

    Stürze, Andrea; Mervart, Leos; Weber, Georg; Rülke, Axel; Wiesensarter, Erwin; Neumaier, Peter

    2016-04-01

    A new version of the BKG Ntrip Client (BNC) has been released. Originally developed in cooperation of the Federal Agency for Cartography and Geodesy (BKG) and the Czech Technical University (CTU) with a focus on multi-stream real-time access to GPS observations, the software has once again been substantially extended. Promoting Open Standards as recommended by the Radio Technical Commission for Maritime Services (RTCM) remains the prime subject. Beside its Graphical User Interface (GUI), the real-time software for Windows, Linux, Mac, and Linux platforms now comes with complete Command Line Interface (CLI) and considerable post processing functionality. RINEX Version 3 file editing & Quality Check (QC) with full support of Galileo, BeiDou, and SBAS - besides GPS and GLONASS - is part of the new features. Comparison of satellite orbit/clock files in SP3 format is another fresh ability of BNC. Simultaneous multi-station Precise Point Positioning (PPP) for real-time displacement-monitoring of entire reference station networks is one more recent addition to BNC. Implemented RTCM messages for PPP (under development) comprise satellite orbit and clock corrections, code and phase observation biases, and the Vertical Total Electron Content (VTEC) of the ionosphere. The well established, mature codebase is mostly written in C++ language. Its publication under GNU GPL is thought to be well-suited for test, validation and demonstration of new approaches in precise real-time satellite navigation when IP streaming is involved. The poster highlights BNC features which are new in version 2.12 and beneficial to IAG institutions and services such as IGS/RT-IGS and to the interested public in general.

  3. Inhibition of smooth muscle contraction and platelet aggregation by peptide 204–212 of lipocortin 5: an attempt to define some structure requirements

    Directory of Open Access Journals (Sweden)

    K. G. Mugridge

    1993-01-01

    Full Text Available Peptide 204–212 of lipocortin (LC 5 inhibited porcine pancreatic phospholipase A2 (PLA2 induced rat stomach strip contractions and ADP induced rabbit platelet aggregation in a concentration dependent manner (IC30 of 10 μM and 400 μM, respectively. The first two amino acids are not necessary since the eptapeptide 206–212 was equipotent in both assays (IC30 of 12.5 μM and 420 μM. Of the two pentapeptides 204–208 and 208–212 only the latter showed inhibitory activity in both models although the potency was much reduced (IC30 of 170 μM and 630 μM compared with that of the parent nonapeptide. Comparison of peptide 204–212 effects with those of its analogues on LC1 and LC2 indicate that lysine 208 and aspartic acid 211 are essential in order to maintain a fully active nonapeptide.

  4. Oxygen Sensing via the Ethylene Response Transcription Factor RAP2.12 Affects Plant Metabolism and Performance under Both Normoxia and Hypoxia1[OPEN

    Science.gov (United States)

    Paul, Melanie Verena; Iyer, Srignanakshi; Lehmann, Martin

    2016-01-01

    Subgroup-VII-ethylene-response-factor (ERF-VII) transcription factors are involved in the regulation of hypoxic gene expression and regulated by proteasome-mediated proteolysis via the oxygen-dependent branch of the N-end-rule pathway. While research into ERF-VII mainly focused on their role to regulate anoxic gene expression, little is known on the impact of this oxygen-sensing system in regulating plant metabolism and growth. By comparing Arabidopsis (Arabidopsis thaliana) plants overexpressing N-end-rule-sensitive and insensitive forms of the ERF-VII-factor RAP2.12, we provide evidence that oxygen-dependent RAP2.12 stability regulates central metabolic processes to sustain growth, development, and anoxic resistance of plants. (1) Under normoxia, overexpression of N-end-rule-insensitive Δ13RAP2.12 led to increased activities of fermentative enzymes and increased accumulation of fermentation products, which were accompanied by decreased adenylate energy states and starch levels, and impaired plant growth and development, indicating a role of oxygen-regulated RAP2.12 degradation to prevent aerobic fermentation. (2) In Δ13RAP2.12-overexpressing plants, decreased carbohydrate reserves also led to a decrease in anoxic resistance, which was prevented by external Suc supply. (3) Overexpression of Δ13RAP2.12 led to decreased respiration rates, changes in the levels of tricarboxylic acid cycle intermediates, and accumulation of a large number of amino acids, including Ala and γ-amino butyric acid, indicating a role of oxygen-regulated RAP2.12 abundance in controlling the flux-modus of the tricarboxylic acid cycle. (4) The increase in amino acids was accompanied by increased levels of immune-regulatory metabolites. These results show that oxygen-sensing, mediating RAP2.12 degradation is indispensable to optimize metabolic performance, plant growth, and development under both normoxic and hypoxic conditions. PMID:27372243

  5. Genome Wide Gene by Environment Interaction Analysis Identifies Common SNPs at 17q21.2 that Are Associated with Increased Body Mass Index Only among Asthmatics

    Science.gov (United States)

    2015-12-16

    general and abdominal weight status among Norwegian adolescents and young adults: the HUNT Study. Pediatric obesity. 2015; 10(5):345–52. doi: 10.1111...Identifies Common SNPs at 17q21.2 that Are Associated with Increased Body Mass Index Only among Asthmatics 5a. CONTRACT NUMBER 5b. GRANT...Environment Interaction Analysis Identifies Common SNPs at 17q21.2 that Are Associated with Increased Body Mass Index Only among Asthmatics LeyaoWang

  6. miR-212 downregulation contributes to the protective effect of exercise against non-alcoholic fatty liver via targeting FGF-21.

    Science.gov (United States)

    Xiao, Junjie; Bei, Yihua; Liu, Jingqi; Dimitrova-Shumkovska, Jasmina; Kuang, Dapeng; Zhou, Qiulian; Li, Jin; Yang, Yanning; Xiang, Yang; Wang, Fei; Yang, Changqing; Yang, Wenzhuo

    2016-02-01

    Non-alcoholic fatty liver disease (NAFLD) is associated with obesity and lifestyle, while exercise is beneficial for NAFLD. Dysregulated microRNAs (miRs) control the pathogenesis of NAFLD. However, whether exercise could prevent NAFLD via targeting microRNA is unknown. In this study, normal or high-fat diet (HF) mice were either subjected to a 16-week running program or kept sedentary. Exercise attenuated liver steatosis in HF mice. MicroRNA array and qRT-PCR demonstrated that miR-212 was overexpressed in HF liver, while reduced by exercise. Next, we investigated the role of miR-212 in lipogenesis using HepG2 cells with/without long-chain fatty acid treatment (± FFA). FFA increased miR-212 in HepG2 cells. Moreover, miR-212 promoted lipogenesis in HepG2 cells (± FFA). Fibroblast growth factor (FGF)-21, a key regulator for lipid metabolism, was negatively regulated by miR-212 at protein level in HepG2 cells. Meanwhile, FFA downregulated FGF-21 both at mRNA and protein levels in HepG2 cells. Also, FGF-21 protein level was reduced in HF liver, while reversed by exercise in vivo. Furthermore, siRNA-FGF-21 abolished the lipogenesis-reducing effect of miR-212 inhibitor in HepG2 cells (± FFA), validating FGF-21 as a target gene of miR-212. These data link the benefit of exercise and miR-212 downregulation in preventing NAFLD via targeting FGF-21.

  7. Different susceptibility of colon cancer DLD-1 and LOVO cell lines to apoptosis induced by DMU-212, a synthetic resveratrol analogue.

    Science.gov (United States)

    Piotrowska, Hanna; Myszkowski, Krzysztof; Amarowicz, Ryszard; Murias, Marek; Kulcenty, Katarzyna; Wierzchowski, Marcin; Jodynis-Liebert, Jadwiga

    2013-12-01

    The cytotoxic activity of DMU-212 has been shown to vary in cell lines derived from the same type of cancer, i.e. ovarian, breast and colorectal ones. However, the molecular mechanism of DMU-212 cytotoxicity has not been clarified in colon cancer cells. This study aims to elucidate the mechanism of antitumor effects of DMU-212 in two human colon cancer cell lines, DLD-1 and LOVO. We showed the stronger cytotoxic activity in DLD-1 cells in which DMU-212 evoked a greater pro-apoptotic effect as compared to that of LOVO cells. The analysis of the expression pattern of 84 apoptosis-related genes indicated transcripts specific to the mitochondria-mediated apoptosis pathway in both colon cancer cell lines used. We found that DMU-212 caused up-regulation of pro-apoptotic Bak1, Bok, Bik, Noxa, Bad, Bax, p53 and Apaf1 transcripts level in DLD-1 cell line, whereas anti-apoptotic Bcl-2, Bcl-xL and Bag1 mRNA expression was decreased. Changes in apoptosis-related genes expression were less pronounced in LOVO cells which did not express CYP1B1 protein and showed lower expression of CYP1A1 protein level than that in DLD-1 cells. Our results suggest that anticancer activity of DMU-212 is closely related to its biotransformation catalysed by these cytochrome P450 isoenzymes.

  8. [A commentary on the Ruling of the Tribuna Constitucional 212/1996 of 19 December 1996 (I)].

    Science.gov (United States)

    González Morán, L

    1998-01-01

    This article is a commentary on Spain's Constitutional Court's ruling of 19 December 1996 (STC 212/1996), on the challenge (596/89) on grounds of alleged unconstitutionality made against Law 42/1988, 28 December, which regulates the donation of human embryos and foetuses or the cells, tissues and organs therefrom. The article is structured as follows: it opens with a summary of Law 42/1988, since this is felt necessary to understand the subsequent challenge made on grounds of alleged unconstitutionality. We then provide specific details of the challenge and the resulting ruling, before concluding with some critical remarks on the aforementioned Law and ruling.

  9. The faithful and the rulers: Calvin's two sermons of 1554 on 1 Timothy 2:1-2

    Directory of Open Access Journals (Sweden)

    A. D. Pont

    1998-01-01

    Full Text Available In this study Calvin's two sermons on 1 Timothy 2:1-2 are examined and the contents briely analyzed. The conclusion is drawn that Calvin is not giving a treatise on the biblical doctrine on princes and rulers, but is addressing the faithful. In doing so, he explains to the congregation their position vis-à-vis the rulers and what Paul's injunctions mean intheir situation. It is true preaching as Calvin only expounds the Biblical message as it is found in this text.

  10. CA4DC2-12E4柴油机的机油耗开发%Oil Consumption Development on CA4DC2-12E4 Engine

    Institute of Scientific and Technical Information of China (English)

    王启航; 金喆; 吴彩丽; 张松涛; 韩祖豪

    2014-01-01

    对于CA4DC2-12E4国Ⅳ排放柴油机的机油耗开发,它是通过控制缸孔变形,优化缸套网纹和改进活塞环的措施来实现。试验结果表明:优化后的样机标定功率机油燃油比为0.1%,满足工程目标0.2%的要求。%To development China IV compliant diesel engine of CA4DC2-12E4, measures were taken of controlling cylinder bore distortion, optimizing cylinder liner crosshatch parameters and developing piston oil ring, and then relevant tests were made to verify the effect of the measures. The results show that the oil/fuel at rated power is 0.1%, much lower than the project target of 0.2%.

  11. Sperm FISH analysis of a 46,XY,t(3;6)(p24;p21.2),inv (8)(p11;2q21.2) double chromosomal rearrangement.

    Science.gov (United States)

    Ferfouri, Fatma; Boitrelle, Florence; Tapia, Sylvie; Molina Gomes, Denise; Selva, Jacqueline; Vialard, François

    2012-02-01

    A complex chromosome rearrangement (CCR) can be defined as a structural chromosomal aberration that involves at least three breakpoints located on two or more chromosomes. Highly unbalanced gametes may lead to infertility or congenital malformations. Here is reported a double rearrangement considered as the simplest possible CCR and, in a sense, not a true CCR, meiotic segregation for a 46,XY,t(3;6)(p24;p21.2),inv(8)(p11;2q21.2) male patient referred after his partner had undergone three early miscarriages. Sperm fluorescence in-situ hybridization was used to screen for translocation and inversion segregation and an interchromosomal effect (ICE) for 13 chromosomes not involved in CCR. The malsegregation rates for the reciprocal translocation and pericentric inversion were 61.2% and 1.7%, respectively. ICE analysis revealed that the observed chromosome aneuploidy rates of between 0.1% and 0.8% did not differ significantly from control values. A slight increase in cumulative ICE (P=0.049) was observed in the patient, relative to control spermatozoa (with rates of 4.6% and 3.1%). The sperm DNA fragmentation rate differed significantly from control values (5.0%; P=0.001). Reciprocal translocation had no impact on meiotic segregation of the pericentric inversion in this double rearrangement. No conclusion could be drawn regarding the impact of pericentric inversion on translocation.

  12. Quantum chemical calculations of anion complex [B12Hx(NF2)12-x]2-, x = 9 – 12

    Science.gov (United States)

    Koblova, E. A.; Saldin, V. I.; Ustinov, A. Yu

    2017-01-01

    The geometric, energetic, spectral and electronic properties of various isomers of B12Hх(NF2)12-х 2‑ anion complex with x = 9 ‑ 12 have been studied using Density Functional Theory (B3LYP/6-311++G**). It was shown that the most stable isomers are characterized by the preference to form the most symmetric structures with uniformly distributed charge densities. However, when replacing a hydrogen atom with difluoramino group, an inductive effect occurs. NF2 group pulls a part of electron density that leads to the polarization of the boron core. Blue shifts in the IR spectrum compared to the vibrations of the free radical NF2 ranging from 5 to 69 cm‑1 for the most stable isomers with the minimum total energy are characteristic and points to the stability of B12Hх(NF2)12-х 2‑ anions. The obtained results broaden the idea of aromaticity of B12H12 2‑ anion and will be useful in synthesis of new B12H12 2‑ derivatives.

  13. Sodium, Saturated Fat, and Sugar Added Intake of The Diet of Children 2-12 Years Old

    Directory of Open Access Journals (Sweden)

    Angga Hardiansyah

    2015-01-01

    Full Text Available The aims of the study were to analyze intake of sodium, saturated fat (SFA, and added sugar of children 2-12 years old. For this purpose, 38.890 children 2-12 years old from the food consumption data of the basic health survey of the Ministry of Health were analyzed. The intake of sodium, SFA, and added sugar were calculated by using food composition table (FCT of Indonesia and USDA, and from nutrition facts of labeled foods.The sodium and SFA calculated include both natural resources and which added to food and beverage products. The sugar added calculated include all sugar which added to food and beverage products. The results showed that the intake of sodium, SFA, and added sugar of each childern varies greatly. The mean of sodium, SFA, and added sugar intake was 1010.3 ± 963.5 mg, 10.9 ± 9,2 g, and 19.0 ± 35.1 g respectively. There were 30% of childern have excessive sodium intake, 28% of childern have excessive SFA intake (> 8%-e, and 14% of childern have excessive added sugar intake (> 10%-e. This implies that some childern exposed to high intake of sodium, saturated fat, and added sugar. 

  14. De-repression of FOXO3a death axis by microRNA-132 and -212 causes neuronal apoptosis in Alzheimer's disease.

    Science.gov (United States)

    Wong, Hon-Kit Andus; Veremeyko, Tatiana; Patel, Nehal; Lemere, Cynthia A; Walsh, Dominic M; Esau, Christine; Vanderburg, Charles; Krichevsky, Anna M

    2013-08-01

    Alzheimer's disease (AD) is a multifactorial and fatal neurodegenerative disorder for which the mechanisms leading to profound neuronal loss are incompletely recognized. MicroRNAs (miRNAs) are recently discovered small regulatory RNA molecules that repress gene expression and are increasingly acknowledged as prime regulators involved in human brain pathologies. Here we identified two homologous miRNAs, miR-132 and miR-212, downregulated in temporal cortical areas and CA1 hippocampal neurons of human AD brains. Sequence-specific inhibition of miR-132 and miR-212 induces apoptosis in cultured primary neurons, whereas their overexpression is neuroprotective against oxidative stress. Using primary neurons and PC12 cells, we demonstrate that miR-132/212 controls cell survival by direct regulation of PTEN, FOXO3a and P300, which are all key elements of AKT signaling pathway. Silencing of these three target genes by RNAi abrogates apoptosis caused by the miR-132/212 inhibition. We further demonstrate that mRNA and protein levels of PTEN, FOXO3a, P300 and most of the direct pro-apoptotic transcriptional targets of FOXO3a are significantly elevated in human AD brains. These results indicate that the miR-132/miR-212/PTEN/FOXO3a signaling pathway contributes to AD neurodegeneration.

  15. Study of the damage induced in the DNA by 21,2 nm X-UV laser radiation; Etude des dommages induits dans l'ADN par irradiation laser X-UV a 21.2

    Energy Technology Data Exchange (ETDEWEB)

    Cassou, K.; Ros, D.; Kazamias, S.; Klinsnick, A.; Jamelot, G.; Guilbaud, O. [Paris-11 Univ., Lab. d' Interaction du Rayonnement X avec la Matiere, LIXAM, CNRS UMR 8624, 91 - Orsay (France); Rus, B.; Kozlova, M.; Polan, J.; Prag, A.R.; Stupka, M. [Institute of Physics, Academy of Sciences of the Czech Republic, Dept. of X-ray Laser/PALS Centre, Prague (Czech Republic); Eot-Houllier, G.; Sage, E. [Paris-11 Univ., Lab. de Genotoxicologie et Cycle Cellulaire, Institut Curie, CNRS UMR 2027, 91 - Orsay (France); Begusova, M.; Stisova, V. [Academy of Sciences of the Czech Republic, Nuclear Physics Institute, Prague (Czech Republic); Penhoat, M.A.H. du; Touati, A.; Chetioui, A. [Paris-6 Univ. et Paris-7 Univ., Groupe de Physique des Solides, CNRS UMR 7588, 75 (France)

    2005-06-01

    We report the preliminary result of the application of the collisional Ne-like soft X-ray laser as radiation source to induce DNA damage. The goal of this experiment was a test bed study of the damage yields induced by soft x-ray radiation in dried plasmid DNA sample. The saturated Ne-like soft x-ray laser available at the PALS facility, delivering several milli-joules in a single 100 ps pulse at 21.2 nm was used to irradiate two different plasmids: pSP189 and pBS. The study is centered on the dose effect leading to single and double strand break in DNA. (authors)

  16. g-factor and quadrupole moment of the 212− isomeric state in 131La: Signature for a weakly-deformed magnetic rotational band head

    Directory of Open Access Journals (Sweden)

    Jasmeet Kaur

    2017-02-01

    Full Text Available The g-factor and the static quadrupole moment of a magnetic rotational band head 212− at 2121 keV in 131La have been determined by means of the time-differential perturbed angular distribution technique. The measured value of the g-factor, +1.060(4, is in agreement with the theoretical value for a three quasi-proton, π3{112−[505]⊗52+[422]⊗52+[413]} Nilsson configuration assignment. The observed spectroscopic quadrupole moment ratio, Qs(212−,131LaQs(192−,137La=0.457(4, supports the collective oblate shape (γ∼−60° with quadrupole deformation β2<0.07. The half-life of the 212− state, 37.2(1 ns, is re-measured with better accuracy.

  17. g-factor and quadrupole moment of the 21/2- isomeric state in 131La: Signature for a weakly-deformed magnetic rotational band head

    Science.gov (United States)

    Kaur, Jasmeet; Bansal, Neeraj; Bhati, A. K.; Kumar, R.; Sharma, Vijay R.; Kapoor, K.; Kumar, V.; Kaur, Navneet

    2017-02-01

    The g-factor and the static quadrupole moment of a magnetic rotational band head 21/2- at 2121 keV in 131La have been determined by means of the time-differential perturbed angular distribution technique. The measured value of the g-factor, + 1.060 (4), is in agreement with the theoretical value for a three quasi-proton, π3 {11/2- [ 505 ] ⊗5/2+ [ 422 ] ⊗5/2+ [ 413 ] } Nilsson configuration assignment. The observed spectroscopic quadrupole moment ratio, Qs (21/2- ,131 La)/Qs (19/2- ,137 La) = 0.457 (4), supports the collective oblate shape (γ ∼ - 60 °) with quadrupole deformation β2 < 0.07. The half-life of the 21/2- state, 37.2(1) ns, is re-measured with better accuracy.

  18. The Neuroprotective Effect of Cannabinoid Receptor Agonist (WIN55,212-2 in Paraoxon Induced Neurotoxicity in PC12 Cells and N-methyl-D-aspartate Receptor Interaction

    Directory of Open Access Journals (Sweden)

    Hedayat Sahraei

    2010-01-01

    Full Text Available Objective: Considering that cannabinoids protect neurons against neurodegeneration, inthis study, the neuroprotective effect of WIN55,212-2 in paraoxon induced neurotoxicity inPC12 cells and the role of the N-methyl-D-aspartate (NMDA receptor were evaluated.Materials and Methods: In this study PC12 cells were maintained in Dulbecco's modifiedeagle’s medium (DMEM+F12 culture medium supplemented with 10% fetal bovineserum. The cells were treated with paraoxon (200 μM in the presence or absence ofWIN55,212-2 (0.1 μM, NMDA receptor agonist NMDA (100 μM, cannabinoid receptorantagonist AM251 and NMDA receptor antagonist MK801 (1 μM at 15 minutes intervals.After 48 hours of exposure, cellular viability and protein expression of the CB1 receptorwere evaluated in PC12 cells.Results: Following the exposure of PC12 cells to paraoxon (200 μM, a reduction in cellsurvival and protein level of the CB1 receptor was observed (p<0.01. Treatment of thecells with WIN55,212-2 (0.1 μM and NMDA (100 μM prior to paraoxon exposure significantlyelevated cell survival and protein level of the CB1 receptor (p<0.01. Also, AM251(1μM did not inhibit the cell survival and protein level of the CB1 receptor increase inducedby WIN55,212-2 (p<0.001. However, MK801 (1 μM did inhibit cell survival andprotein expression of the CB1 receptor increase induced by NMDA (p<0.001.Conclusion: The results indicate that WIN55,212-2 and NMDA protect PC12 cellsagainst paraoxon induced toxicity. In addition, the neuroprotective effect of WIN55,212-2and NMDA was cannabinoid receptor-independent and NMDA receptor dependent, respectively.

  19. Replacement P212H altered the pH-temperature profile of phytase from Aspergillus niger NII 08121.

    Science.gov (United States)

    Ushasree, Mrudula Vasudevan; Vidya, Jalaja; Pandey, Ashok

    2015-03-01

    Microbial phytase, a widely used animal feed enzyme, needs to be active and stable in the acidic milieu for better performance in the monogastric gut. Aspergillus niger phytases exhibit an activity dip in the pH range from 3.0 to 3.5. Replacement of amino acids, which changed the pKa of catalytic residues H82 and D362, resulted in alteration of the pH profile of a thermostable phytase from A. niger NII 08121. Substitution P212H in the protein loop at 14 Å distance to the active site amended the pH optimum from 2.5 to pH 3.2 nevertheless with a decrease in thermostability than the wild enzyme. This study described the utility of amino acid replacements based on pKa shifts of catalytic acid/base to modulate the pH profile of phytases.

  20. 对式子mgh1-mgh2=1/2mv2的审视及修正

    Institute of Scientific and Technical Information of China (English)

    刘刚; 王文涛; 唐齐林

    2010-01-01

    @@ 自由落体运动是一种我们很熟悉的运动.有一质量为m的物体从地面上方h1高度处自由下落到h2高度,此时物体的速度为v.分析此过程,我们很容易得出这样的式子 mgh1-mgh2=1/2mv2.(1) (1)式看似很严密,然而如果进一步审视和追问就会发现这个式子只是一个近似表达.

  1. Copper(II) mixed chelate compounds induce apoptosis through reactive oxygen species in neuroblastoma cell line CHP-212.

    Science.gov (United States)

    Gutiérrez, Anllely Grizett; Vázquez-Aguirre, Adriana; García-Ramos, Juan Carlos; Flores-Alamo, Marcos; Hernández-Lemus, Enrique; Ruiz-Azuara, Lena; Mejía, Carmen

    2013-09-01

    In the present work we report the antiproliferative activity of Cu(II) coordination compounds, CasIIgly ([Cu(4,7-dimethyl-1,10-phenanthroline) (glycinato) (H2O)]NO3), CasIIIia ([Cu(4,4'-dimethyl-2,2'-bipyridine) (glycinato) (H2O)]NO3), and CasIIIEa ([Cu(4,7-dimethyl-1,10-phenanthroline) (acetylacetonato) (H2O)]NO3), against human tumoral cell line CHP-212 (estromal neuroblastoma). Additionally, the molecular structure of CasIIIEa was reported. The IC50 values obtained for the evaluated compounds are in the range 18 to 47 μM, representing an inhibition potency increase of 5 to 12 times compared with cisplatin (IC50=226.7 μM). After 2h of incubation with the evaluated compounds, cells showed high levels of reactive oxygen species and a considerable GSH depletion, besides an important disruption of the mitochondrial membrane with release of cytochrome C and besides the presence of caspase-3, an effector caspase that is activated in the last step of apoptosis cascade. The results confirm that cell death in neuroblastoma CHP-212 treated with Casiopeínas occurs via apoptosis. Due to the lack of expression of caspase-8, cell death is principally by the mitochondrial pathway. Thus, one of the most interesting findings of this work is the identification of a very important damage in neuroblastoma cells induced by Cu(II) coordination compounds in a very short exposition times. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. Polymorphisms in a Putative Enhancer at the 10q21.2 Breast Cancer Risk Locus Regulate NRBF2 Expression

    DEFF Research Database (Denmark)

    Darabi, Hatef; McCue, Karen; Beesley, Jonathan

    2015-01-01

    Genome-wide association studies have identified SNPs near ZNF365 at 10q21.2 that are associated with both breast cancer risk and mammographic density. To identify the most likely causal SNPs, we fine mapped the association signal by genotyping 428 SNPs across the region in 89,050 European and 12,...

  3. 30 CFR 285.212 - What is the process MMS will follow if there is reason to believe that competitors have withdrawn...

    Science.gov (United States)

    2010-07-01

    ... reason to believe that competitors have withdrawn before the Final Sale Notice is issued? 285.212 Section... to believe that competitors have withdrawn before the Final Sale Notice is issued? The MMS may decide... competitors have withdrawn and competition no longer exists. We will issue a second public notice of...

  4. 血清miR-132和miR-212表达在肝硬化检测中的意义

    Institute of Scientific and Technical Information of China (English)

    王永良

    2015-01-01

    目的:研究血清miR-132和miR-212表达在肝硬化检测中的意义。方法随机选择63例不同阶段的肝硬化患者,参照63例健康人血样,同步使用PCR检测法进行肝功能检测和miR-132和miR-212表达检测,对比相应结果。结果肝硬化患者的肝功能检测结果与健康人士有明显差别,肝硬化患者的miR-132和miR-212表达显著降低,与内参照U6比值分别达到了0.43±0.22和0.73±0.48。结论肝硬化患者的miR-132和miR-212表达会出现显著降低。

  5. Clinical observation on cod liver oil capsules for adjunctive treatment of 212 children with pneumonia%鱼肝油胶囊佐治小儿肺炎212例临床观察

    Institute of Scientific and Technical Information of China (English)

    任淑红; 王杰英; 雷淑琴; 常洁

    2012-01-01

    目的:观察鱼肝油佐治小儿肺炎的临床疗效.方法:将212例小儿肺炎分为支气管肺炎组100例、迁延性肺炎组112例,每组均设治疗组和对照组.结果:支气管肺炎组患儿,治疗组的总有效率为92.00%,对照组总有效率为88.00%,两组比较差异无统计学意义;迁延性肺炎组患儿,治疗组总有效率为89.29%,对照组总有效率为75.00%,两组比较差异有统计学意义.结论:鱼肝油胶囊配合西药治疗小儿迁延性肺炎疗效满意,配合治疗支气管肺炎无明显疗效.%Objective: To observe the clinical efficacy of cod liver oil capsules for adjunctive treatment of children with pneumonia. Methods: A total of 212 children with pneumonia were divided into bronchial pneumonia group (100 children) and prolonged pneumonia group (112 children) , then each group was divided into treatment subgroup and control subgroup. Results: The total effective rates in treatment subgroup and control subgroup of bronchial pneumonia group were 92. 00% and 88. 00% , respectively, there was no statistically significant difference between the two subgroups; the .total effective rates in treatment subgroup and control subgroup of prolonged pneumonia group were 89.29% and 75.00% , respectively, there was statistically significant difference between the two subgroups. Conclusion; The curative effect of cod liver oil capsules combined with Western medicine is satisfactory for treatment of prolonged pneumonia in children, but the curative effect is not obvious for treatment of bronchial pneumonia.

  6. WIN 55,212-2, agonist of cannabinoid receptors, prevents amyloid β1-42 effects on astrocytes in primary culture.

    Science.gov (United States)

    Aguirre-Rueda, Diana; Guerra-Ojeda, Sol; Aldasoro, Martin; Iradi, Antonio; Obrador, Elena; Mauricio, Maria D; Vila, Jose M; Marchio, Patricia; Valles, Soraya L

    2015-01-01

    Alzheimer's disease (AD), a neurodegenerative illness involving synaptic dysfunction with extracellular accumulation of Aβ1-42 toxic peptide, glial activation, inflammatory response and oxidative stress, can lead to neuronal death. Endogenous cannabinoid system is implicated in physiological and physiopathological events in central nervous system (CNS), and changes in this system are related to many human diseases, including AD. However, studies on the effects of cannabinoids on astrocytes functions are scarce. In primary cultured astrocytes we studied cellular viability using MTT assay. Inflammatory and oxidative stress mediators were determined by ELISA and Western-blot techniques both in the presence and absence of Aβ1-42 peptide. Effects of WIN 55,212-2 (a synthetic cannabinoid) on cell viability, inflammatory mediators and oxidative stress were also determined. Aβ1-42 diminished astrocytes viability, increased TNF-α and IL-1β levels and p-65, COX-2 and iNOS protein expression while decreased PPAR-γ and antioxidant enzyme Cu/Zn SOD. WIN 55,212-2 pretreatment prevents all effects elicited by Aβ1-42. Furthermore, cannabinoid WIN 55,212-2 also increased cell viability and PPAR-γ expression in control astrocytes. In conclusion cannabinoid WIN 55,212-2 increases cell viability and anti-inflammatory response in cultured astrocytes. Moreover, WIN 55,212-2 increases expression of anti-oxidant Cu/Zn SOD and is able to prevent inflammation induced by Aβ1-42 in cultured astrocytes. Further studies would be needed to assess the possible beneficial effects of cannabinoids in Alzheimer's disease patients.

  7. WIN 55,212-2, agonist of cannabinoid receptors, prevents amyloid β1-42 effects on astrocytes in primary culture.

    Directory of Open Access Journals (Sweden)

    Diana Aguirre-Rueda

    Full Text Available Alzheimer's disease (AD, a neurodegenerative illness involving synaptic dysfunction with extracellular accumulation of Aβ1-42 toxic peptide, glial activation, inflammatory response and oxidative stress, can lead to neuronal death. Endogenous cannabinoid system is implicated in physiological and physiopathological events in central nervous system (CNS, and changes in this system are related to many human diseases, including AD. However, studies on the effects of cannabinoids on astrocytes functions are scarce. In primary cultured astrocytes we studied cellular viability using MTT assay. Inflammatory and oxidative stress mediators were determined by ELISA and Western-blot techniques both in the presence and absence of Aβ1-42 peptide. Effects of WIN 55,212-2 (a synthetic cannabinoid on cell viability, inflammatory mediators and oxidative stress were also determined. Aβ1-42 diminished astrocytes viability, increased TNF-α and IL-1β levels and p-65, COX-2 and iNOS protein expression while decreased PPAR-γ and antioxidant enzyme Cu/Zn SOD. WIN 55,212-2 pretreatment prevents all effects elicited by Aβ1-42. Furthermore, cannabinoid WIN 55,212-2 also increased cell viability and PPAR-γ expression in control astrocytes. In conclusion cannabinoid WIN 55,212-2 increases cell viability and anti-inflammatory response in cultured astrocytes. Moreover, WIN 55,212-2 increases expression of anti-oxidant Cu/Zn SOD and is able to prevent inflammation induced by Aβ1-42 in cultured astrocytes. Further studies would be needed to assess the possible beneficial effects of cannabinoids in Alzheimer's disease patients.

  8. microRNA-132/212 deficiency enhances Aβ production and senile plaque deposition in Alzheimer’s disease triple transgenic mice

    Science.gov (United States)

    Hernandez-Rapp, Julia; Rainone, Sara; Goupil, Claudia; Dorval, Véronique; Smith, Pascal Y.; Saint-Pierre, Martine; Vallée, Maxime; Planel, Emmanuel; Droit, Arnaud; Calon, Frédéric; Cicchetti, Francesca; Hébert, Sébastien S.

    2016-01-01

    The abnormal regulation of amyloid-β (Aβ) metabolism (e.g., production, cleavage, clearance) plays a central role in Alzheimer’s disease (AD). Among endogenous factors believed to participate in AD progression are the small regulatory non-coding microRNAs (miRs). In particular, the miR-132/212 cluster is severely reduced in the AD brain. In previous studies we have shown that miR-132/212 deficiency in mice leads to impaired memory and enhanced Tau pathology as seen in AD patients. Here we demonstrate that the genetic deletion of miR-132/212 promotes Aβ production and amyloid (senile) plaque formation in triple transgenic AD (3xTg-AD) mice. Using RNA-Seq and bioinformatics, we identified genes of the miR-132/212 network with documented roles in the regulation of Aβ metabolism, including Tau, Mapk, and Sirt1. Consistent with these findings, we show that the modulation of miR-132, or its target Sirt1, can directly regulate Aβ production in cells. Finally, both miR-132 and Sirt1 levels correlated with Aβ load in humans. Overall, our results support the hypothesis that the miR-132/212 network, including Sirt1 and likely other target genes, contributes to abnormal Aβ metabolism and senile plaque deposition in AD. This study strengthens the importance of miR-dependent networks in neurodegenerative disorders, and opens the door to multifactorial drug targets of AD by targeting Aβ and Tau. PMID:27484949

  9. Effect of cannaboid WIN55,212-2 on the expression of cAMP in bovine trabecular meshwork cells in viryo%大麻素WIN55,212-2对体外培养牛眼小梁细胞cAMP表达的影响

    Institute of Scientific and Technical Information of China (English)

    秦维山; 王万辉; 路一凡

    2012-01-01

    目的 通过研究大麻素WIN55,212-2对体外培养牛眼小梁细胞(BTMC)环腺苷酸(cAMP)表达的影响,探讨其增加房水排出而降低眼压的机制.方法 对BTMC进行体外原代培养及传代培养,应用免疫组化的方法鉴定细胞.然后用不同浓度的WIN55,212-2作用于BTMC,放射免疫法检测细胞上清液中cAMP的水平.结果 WIN55,212-2浓度在0.1-20μmol/L范围内,培养液中cAMP水平随浓度增加而显著性升高,若WIN55,212-2浓度继续增高(40-60μmol/L),cAMP水平不再升高.结论 WIN55,212-2可能通过提高BTMC内cAMP水平而起到降低眼压的作用.

  10. Production of actinium-225 for alpha particle mediated radioimmunotherapy.

    Science.gov (United States)

    Boll, Rose A; Malkemus, Dairin; Mirzadeh, Saed

    2005-05-01

    The initial clinical trials for treatment of acute myeloid leukemia have demonstrated the effectiveness of the alpha emitter (213)Bi in killing cancer cells. Bismuth-213 is obtained from a radionuclide generator system from decay of 10-days (225)Ac parent. Recent pre-clinical studies have also shown the potential application of both (213)Bi, and the (225)Ac parent radionuclide in a variety of cancer systems and targeted radiotherapy. This paper describes our five years of experience in production of (225)Ac in partial support of the on-going clinical trials. A four-step chemical process, consisting of both anion and cation exchange chromatography, is utilized for routine separation of carrier-free (225)Ac from a mixture of (228)Th, (229)Th and (232)Th. The separation of Ra and Ac from Th is achieved using the marcoporous anion exchange resin MP1 in 8M HNO(3) media. Two sequential MP1/NO(3) columns provide a separation factor of approximately 10(6) for Ra and Ac from Th. The separation of Ac from Ra is accomplished on a low cross-linking cation exchange resin AG50-X4 using 1.2M HNO(3) as eluant. Two sequential AG50/NO(3) columns provide a separation factor of approximately 10(2) for Ac from Ra. A 60-day processing schedule has been adopted in order to reduce the processing cost and to provide the highest levels of (225)Ac possible. Over an 8-week campaign, a total of approximately 100 mCi of (225)Ac (approximately 80% of the theoretical yield) is shipped in 5-6 batches, with the first batch typically consisting of approximately 50 mCi. After the initial separation and purification of Ac, the Ra pool is re-processed on a bi-weekly schedule or as needed to provide smaller batches of (225)Ac. The averaged radioisotopic purity of the (225)Ac was 99.6 +/- 0.7% with a (225)Ra content of < or =0.6%, and an average (229)Th content of (4(-4)(+5)) x 10(-5)%.

  11. Isolation of Actinium from Neutron-irradiated Thorium-I

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    Isolation of Actinium from Neutron-irradiated Thorium-I¥YangWeifan;YuanShuanggui;MuWantong;ZhangXueqian;LiZhongweiandZhaoLili...

  12. Design of Brushless DC Motor Control System Based on R5F212L4%基于R5F212L4的无刷直流电动机控制系统设计

    Institute of Scientific and Technical Information of China (English)

    吴军; 钟名宏; 杜泽华

    2013-01-01

    针对有霍尔无刷直流电动机,提出一种以R5F212L4为核心,基于速度反馈PI控制、位置反馈及相电流相位反馈三闭环SVPWM的驱动系统.提出系统的控制策略及硬件实现;分析传感器安装误差对转子角度测量的影响,提出可以减小测量误差的计算方法;设计并实现系统硬件电路及程序调试.实验证明系统性能稳定,噪声小,实现无级调速等优点,同时可以方便地添加所需功能模块,在中小功率无刷直流电动机应用,如空调、冰箱、风扇等高端家用电器中有明显优势.

  13. Presynaptic effects of anandamide and WIN55,212-2 on glutamatergic nerve endings isolated from rat hippocampus.

    Science.gov (United States)

    Cannizzaro, C; D'Amico, M; Preziosi, P; Martire, M

    2006-02-01

    We examined the effects of the endocannabinoide-anandamide (AEA), the synthetic cannabinoid, WIN55,212-2, and the active phorbol ester, 4-beta-phorbol 12-myristate 13-acetate (4-beta-PMA), on the release of [(3)H]d-Aspartate ([(3)H]d-ASP) from rat hippocampal synaptosomes. Release was evoked with three different stimuli: (1) KCl-induced membrane depolarization, which activates voltage-dependent Ca(2+) channels and causes limited neurotransmitter exocytosis, presumably from ready-releasable vesicles docked in the active zone; (2) exposure to the Ca(2+) ionophore-A23187, which causes more extensive transmitter release, presumably from intracellular reserve vesicles; and (3) K(+) channel blockade by 4-aminopyridine (4-AP), which generates repetitive depolarization that stimulates release from both ready-releasable and reserve vesicles. AEA produced concentration-dependent inhibition of [(3)H]d-ASP release stimulated with 15 mM KCl (E(max)=47.4+/-2.8; EC(50)=0.8 microM) but potentiated the release induced by 4-AP (1mM) (+22.0+/-1.3% at 1 microM) and by A23187 (1 microM) (+98.0+/-5.9% at 1 microM). AEA's enhancement of the [(3)H]d-ASP release induced by the Ca(2+) ionophore was mimicked by 4-beta-PMA, which is known to activate protein kinase C (PKC), and the increases produced by both compounds were completely reversed by synaptosome treatment with staurosporine (1 microM), a potent PKC blocker. In contrast, WIN55,212-2 inhibited the release of [(3)H]d-ASP evoked by KCl (E(max)=47.1+/-2.8; EC(50)=0.9 microM) and that produced by 4-AP (-26.0+/-1.5% at 1 microM) and had no significant effect of the release induced by Ca(2+) ionophore treatment. AEA thus appears to exert a dual effect on hippocampal glutamatergic nerve terminals. It inhibits release from ready-releasable vesicles and potentiates the release observed during high-frequency stimulation, which also involves the reserve vesicles. The latter effect is mediated by PKC. These findings reveal novel effects of AEA

  14. Functional responses to the cannabinoid agonist WIN 55,212-2 in neonatal rats of both genders: influence of weaning.

    Science.gov (United States)

    Borcel, Erika; Pérez-Alvarez, Laura; de Ceballos, María L; Ramirez, Belén G; Marco, Eva Maria; Fernández, Beatriz; Rubio, Marina; Guaza, Carmen; Viveros, Ma-Paz

    2004-07-01

    We have studied behavioural, biochemical and endocrine responses to the cannabinoid agonist WIN 55,212-2 (WIN) in neonatal rats, as well as the effects of weaning on such responses. We used preweanling rats (20 days of age), 25-day-old weaned rats (weaning at Day 22) and 25-day-old nonweaned rats of both sexes. The behavioural effects of WIN were assessed in the nociceptive tail immersion test and in the open field. We also analysed the effect of weaning on corticosterone responses to WIN (radioimmunoassay) as well as on WIN-stimulated [35S] GTPgammaS binding in periaqueductal grey (PAG) and striatum. The cannabinoid agonist induced a modest increase in pain thresholds, whereas the effect of the drug on open-field activity, particularly on vertical activity, was much more marked. The weaning process appeared to reduce the baseline nociceptive latencies of the female rats. No significant effect of weaning on the behavioural responses to WIN was found. However, the group of weaned females (but not males) showed a significantly reduced WIN-stimulated [35S] GTPgammaS binding in the striatum. The cannabinoid agonist significantly increased the corticosterone levels of 25-day-old rats with the effect being more marked in weaned than in nonweaned animals. The results suggest that the weaning process might produce some sexually dimorphic developmental changes in CB1 receptor function.

  15. Measuring Progress on the Control of Porcine Reproductive and Respiratory Syndrome (PRRS) at a Regional Level: The Minnesota N212 Regional Control Project (Rcp) as a Working Example.

    Science.gov (United States)

    Valdes-Donoso, Pablo; Jarvis, Lovell S; Wright, Dave; Alvarez, Julio; Perez, Andres M

    2016-01-01

    Due to the highly transmissible nature of porcine reproductive and respiratory syndrome (PRRS), implementation of regional programs to control the disease may be critical. Because PRRS is not reported in the US, numerous voluntary regional control projects (RCPs) have been established. However, the effect of RCPs on PRRS control has not been assessed yet. This study aims to quantify the extent to which RCPs contribute to PRRS control by proposing a methodological framework to evaluate the progress of RCPs. Information collected between July 2012 and June 2015 from the Minnesota Voluntary Regional PRRS Elimination Project (RCP-N212) was used. Demography of premises (e.g. composition of farms with sows = SS and without sows = NSS) was assessed by a repeated analysis of variance. By using general linear mixed-effects models, active participation of farms enrolled in the RCP-N212, defined as the decision to share (or not to share) PRRS status, was evaluated and used as a predictor, along with other variables, to assess the PRRS trend over time. Additionally, spatial and temporal patterns of farmers' participation and the disease dynamics were investigated. The number of farms enrolled in RCP-N212 and its geographical coverage increased, but the proportion of SS and NSS did not vary significantly over time. A significant increasing (pRCP-N212, active participation is not ensured. By evaluating the effect of participation on the occurrence of PRRS, the value of sharing information among producers may be demonstrated, in turn justifying the existence of RCPs.

  16. Patio 2.12: Vivienda prefabricada, sostenible, autosuficiente y energéticamente eficiente. Participación en la competición Solar Decathlon Europe 2012

    Directory of Open Access Journals (Sweden)

    Terrados-Cepeda, F. J.

    2015-06-01

    Full Text Available The prototype “Patio 2.12” was Andalucía Team’s proposal for the 2012 Solar Decathlon Competition (SDE 2012, that was held in Madrid during September 2012, where it was awarded several prizes like the first one in Energy Efficiency. The team comprised a group of teachers and students from four Andalusian Universities. It worked for two years in the design and construction of a housing prototype that could be an example of several concepts at the same time: energy efficiency, architectural integration of solar systems, intermediate scale of prefabrication and a today´s interpretation of traditional Mediterranean way of living and building. Patio 2.12 was intended to be a compendium of energy, sustainability and passive conditioning devices.El prototipo Patio 2.12 fue la propuesta del equipo Andalucía Team para la competición Solar Decathlon Europe 2012 (SDE 2012, celebrada en Madrid en septiembre de 2012, en la que recibió hasta 9 premios, entre los que figura el de Eficiencia Energética. El equipo, integrado por profesores y alumnos de cuatro universidades andaluzas, trabajó durante dos años en el diseño y construcción de un prototipo de vivienda basada en la eficiencia energética, en la integración arquitectónica de los sistemas solares, en la prefabricación de escala intermedia y en una reinterpretación de la forma de vivir y construir de la tradición mediterránea. Patio 2.12 pretende ser un compendio de energía, sostenibilidad y mecanismos de acondicionamiento pasivo.

  17. Neuroprotective effect of WIN55,212-2 against 3-nitropropionic acid-induced toxicity in the rat brain: involvement of CB1 and NMDA receptors

    Science.gov (United States)

    Maya-López, Marisol; Colín-González, Ana Laura; Aguilera, Gabriela; de Lima, María Eduarda; Colpo-Ceolin, Ana; Rangel-López, Edgar; Villeda-Hernández, Juana; Rembao-Bojórquez, Daniel; Túnez, Isaac; Luna-López, Armando; Lazzarini-Lechuga, Roberto; González-Puertos, Viridiana Yazmín; Posadas-Rodríguez, Pedro; Silva-Palacios, Alejandro; Königsberg, Mina; Santamaría, Abel

    2017-01-01

    The endocannabinoid system (ECS), and agonists acting on cannabinoid receptors (CBr), are known to regulate several physiological events in the brain, including modulatory actions on excitatory events probably through N-methyl-D-aspartate receptor (NMDAr) activity. Actually, CBr agonists can be neuroprotective. The synthetic CBr agonist WIN55,212-2 acts mainly on CB1 receptor. In turn, the mitochondrial toxin 3-nitropropionic acid (3-NP) produces striatal alterations in rats similar to those observed in the brain of Huntington’s disease patients. Herein, the effects of WIN55,212-2 were tested on different endpoints of the 3-NP-induced toxicity in rat brain synaptosomes and striatal tissue. Motor activity was also evaluated. The 3-NP (1 mM)-induced mitochondrial dysfunction and lipid peroxidation was attenuated by WIN55,212-2 (1 µM) in synaptosomal fractions. The intrastriatal bilateral injection of 3-NP (500 nmol/µL) to rats increased lipid peroxidation and locomotor activity, augmented the rate of cell damage, and decreased the striatal density of neuronal cells. These alterations were accompanied by transcriptional changes in the NMDA (NR1 subunit) content. The administration of WIN55212-2 (1 mg/kg, i.p.) to rats for six consecutive days, before the 3-NP injection, exerted preventive effects on all alterations elicited by the toxin. The prevention of the 3-NP-induced NR1 transcriptional alterations by the CBr agonist together with the increase of CB1 content suggest an early reduction of the excitotoxic process via CBr activation. Our results demonstrate a protective role of WIN55,212-2 on the 3-NP-induced striatal neurotoxicity that could be partially related to the ECS stimulation and induction of NMDAr hypofunction, representing an effective therapeutic strategy at the experimental level for further studies.

  18. 2015年全球印刷设备器材市场规模或达212.2亿美元

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    全球行业分析公司GIA日前公布了对全球印刷设备器材市场的研究报告,结果显示:受数字印刷市场需求增长的影响,全球印刷设备器材市场的规模有望在2015年达到212.2亿美元。

  19. Regulation of heparin-binding EGF-like growth factor by miR-212 and acquired cetuximab-resistance in head and neck squamous cell carcinoma.

    Directory of Open Access Journals (Sweden)

    Hiromitsu Hatakeyama

    Full Text Available BACKGROUND: We hypothesized that chronic inhibition of epidermal growth factor receptor (EGFR by cetuximab, a monoclonal anti-EGFR antibody, induces up-regulation of its ligands resulting in resistance and that microRNAs (miRs play an important role in the ligand regulation in head and neck squamous cell carcinoma (HNSCC. METHODOLOGY/PRINCIPAL FINDINGS: Genome-wide changes in gene and miR expression were determined in cetuximab-sensitive cell line, SCC1, and its resistant derivative 1Cc8 using DNA microarrays and RT-PCR. The effects of differentially expressed EGFR ligands and miRs were examined by MTS, colony formation, ELISA, and western blot assays. Heparin-binding EGF-like growth factor (HB-EGF and its regulator, miR-212, were differentially expressed with statistical significance when SCC1 and 1Cc8 were compared for gene and miR expression. Stimulation with HB-EGF induced cetuximab resistance in sensitive cell lines. Inhibition of HB-EGF and the addition of miR-212 mimic induced cetuximab sensitivity in resistant cell lines. MicroRNA-212 and HB-EGF expression were inversely correlated in an additional 33 HNSCC and keratinocyte cell lines. Six tumors and 46 plasma samples from HNSCC patients were examined for HB-EGF levels. HB-EGF plasma levels were lower in newly diagnosed HNSCC patients when compared to patients with recurrent disease. CONCLUSIONS/SIGNIFICANCE: Increased expression of HB-EGF due to down-regulation of miR-212 is a possible mechanism of cetuximab resistance. The combination of EGFR ligand inhibitors or miR modulators with cetuximab may improve the clinical outcome of cetuximab therapy in HNSCC.

  20. WIN55,212-2, a Cannabinoid Receptor Agonist, Protects Against Nigrostriatal Cell Loss in the MPTP Mouse Model of Parkinson’s Disease

    OpenAIRE

    Price, David A.; Martinez, Alex A; Seillier, Alexandre; Koek, Wouter; Acosta, Yolanda; Fernandez, Elizabeth; Strong, John R.; Lutz, Beat; Marsicano, Giovanni; Roberts, James L.; Giuffrida, Andrea

    2009-01-01

    Parkinson’s disease (PD) is characterized by the progressive loss of nigrostriatal dopamine (DA) neurons leading to motor disturbances and cognitive impairment. Current pharmacotherapies relieve PD symptoms temporarily but fail to prevent or slow down the disease progression. In this study, we investigated the molecular mechanisms by which the non-selective cannabinoid receptor agonist WIN55,212-2 (WIN) protects mouse nigrostriatal neurons from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MP...

  1. The synthetic cannabinoid WIN-55,212 induced-apoptosis in cytotrophoblasts cells by a mechanism dependent on CB1 receptor.

    Science.gov (United States)

    Almada, Marta; Costa, Lia; Fonseca, Bruno Miguel; Amaral, Cristina; Teixeira, Natércia; Correia-da-Silva, Georgina

    2017-06-15

    The endocannabinoid system has evolved as a key regulator in several pathological and physiological processes, including placentation, decidualization and implantation. In addition, it is known that Cannabis and cannabinoids negatively affect female reproduction. Although, the biological action of synthetic cannabinoids, such as WIN-55,212, in human fertility and pregnancy outcome remain to be unveiled. A tight balance between proliferation, differentiation and apoptosis of trophoblast cells is required for placental development and pregnancy outcome. Therefore, in this work, the effects of the synthetic cannabinoid WIN-55,212 in placental cytotrophoblast cells were explored. For that, it was used a human choriocarcinoma cell line, BeWo cells, and primary cultures of human cytotrophoblasts isolated from term placentas. Results demonstrate that this synthetic cannabinoid induces cell cycle arrest. We also observed that cell viability loss was associated with a disruption of mitochondrial membrane potential and activation of caspases -9 and -3/-7 independently of reactive oxygen species (ROS) production or recruitment of the endoplasmic reticulum stress marker CHOP. Moreover, these effects were prevented by pre-incubation with a selective cannabinoid receptor 1 (CBR1) antagonist (AM281). Thus, our results provide strong evidences of the apoptotic process induced by WIN-55,212 through the activation of the CBR1, which may reveal the impact of cannabinoids consumption during placental development. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Pancreatic cancer-derived exosomes transfer miRNAs to dendritic cells and inhibit RFXAP expression via miR-212-3p.

    Science.gov (United States)

    Ding, Guoping; Zhou, Liangjing; Qian, Yingming; Fu, Mingnian; Chen, Jian; Chen, Jionghuang; Xiang, Jianyang; Wu, Zhengrong; Jiang, Guixing; Cao, Liping

    2015-10-06

    It has been reported tumor-derived exosomes can transfer miRNAs to recipient cells in the tumor microenvironment, promoting tumor invasion and metastasis. The present research aimed to explore how pancreatic cancer (PC) derived exosomal miRNAs inhibited mRNA expression of dendritic cells and induced immune tolerance. Our study revealed that 9 PC-related miRNAs were increased and 208 mRNAs were inhibited in exosome-stimulated dendritic cells (exo-iDCs) compared to immature dendritic cells (iDCs). A target prediction between the 9 miRNAs and 208 mRNAs was performed by bioinformatics database analysis. From the target prediction, it was predicted and validated that regulatory factor X-associated protein (RFXAP), an important transcription factor for MHC II, was inhibited by miR-212-3p transferred from PC-secreted exosomes, resulting in decreased MHC II expression. Moreover, a clinical study showed a negative correlation between miR-212-3p and RFXAP in PC tissue. From these data, we concluded that PC-related miRNAs can be transferred to dendritic cells via exosome and inhibit target mRNA expression. More importantly, PC-derived exosomes inhibit RFXAP expression via miR-212-3p, which decrease MHC II expression and induce immune tolerance of dendritic cells. RFXAP deficiency has never been reported in solid tumors. The functions and mechanisms of RFXAP in tumors deserve future explorations.

  3. FDG PET/CT imaging in primary osseous and soft tissue sarcomas: a retrospective review of 212 cases

    Energy Technology Data Exchange (ETDEWEB)

    Charest, Mathieu [Lakeshore General Hospital, Service of Nuclear Medicine, Pointe-Claire (Canada); Hickeson, Marc; Lisbona, Robert; Novales-Diaz, Javier A.; Derbekyan, Vilma [McGill University Health Center, Department of Nuclear Medicine, Montreal (Canada); Turcotte, Robert E. [McGill University Health Center, Department of Orthopedic Surgery, Montreal (Canada)

    2009-12-15

    The aims of this study are to evaluate the sensitivity of FDG PET/CT for detection of soft tissue and osseous sarcomas on the basis of FDG avidity. We retrospectively evaluated 212 consecutive patients with known soft tissue or osseous sarcoma who had undergone a FDG PET/CT study for the initial staging or assessment of recurrence of disease. The maximum standardized uptake value (SUV{sub max}) of each primary and/or most intense metastatic lesion was measured and compared with the histological data provided in the final pathological reports. An SUV{sub max} of 2.5 or greater was considered positive for our analysis. Sufficient histopathological data were available for 160 soft tissue sarcomas and 52 osseous sarcomas. FDG PET/CT detected 93.9% of all sarcomas with a sensitivity of 93.7% for soft tissue sarcomas and 94.6% for osseous sarcomas. The sensitivities of the most common sarcoma histologies were 100% for leiomyosarcomas, 94.7% for osteosarcomas, 100% for Ewing's sarcomas, 88.9% for liposarcomas, 80.0% for synovial sarcomas, 100% for gastrointestinal stromal tumors, 87.5% for malignant peripheral nerve sheath tumors, 100% for fibroblastic and myoblastic sarcomas, and 100% for malignant fibrohistiocytic tumors. The receiver-operating characteristic curve revealed an area under the curve of 94% for the discrimination of low-grade and high-grade sarcomas imaged for initial staging by FDG PET/CT. The combined metabolic and morphological information of FDG PET/CT imaging allows high sensitivity for the detection of various sarcomas and accurate discrimination between newly diagnosed low-grade and high-grade sarcomas. (orig.)

  4. Predictors of shingles reports at diagnosis of common variable immunodeficiency and selective immunoglobulin G subclass deficiency in 212 Alabama adults

    Directory of Open Access Journals (Sweden)

    James C. Barton

    2012-07-01

    Full Text Available We sought to determine predictors of shingles reports in adults with common variable immunodeficiency or immunoglobulin (Ig G subclass deficiency (CVID/IgGSD. We tabulated observations at diagnosis of CVID/IgGSD in 212 white adult index patients (165 women, 47 men who responded to a question about having had shingles. None had been vaccinated for herpes zoster. We analyzed age, sex, and shingles reports; blood levels of CD19+, CD4+, CD8+, and CD56+ mononuclear cells; serum levels of IgG subclasses, IgA, and IgM; and positivity for human leukocyte antigen (HLA-A and -B haplotypes. Cell counts and immunoglobulin levels were normalized with loge (ln transformation for analyses. Thirty-one patients (14.6% reported shingles; 11 reported recurrent or disseminated shingles. Patients with shingles reports had greater mean age at diagnosis of CVID/IgGSD [54±13 (standard deviation years vs. 47±12 years; P=0.0130] and a greater prevalence of HLA-A*01, B*08 positivity (35.5% vs. 17.7%; P=0.0227. In a 13-factor logistic regression model, there was a positive association of age with shingles reports [P=0.0151; odds ratio (1.05, 95% confidence interval 1.01, 1.08]. HLA-A*01, B*08 positivity was also positively associated with shingles reports [P=0.0480; odds ratio 2.61 (1.00, 6.81]. During a mean followup interval of 7.5 years after CVID/IgGSD diagnosis, the prevalence of recurrent shingles was almost five-fold greater in patients with previous shingles reports. In conclusion, in white adults at CVID/IgGSD diagnosis, age at diagnosis and positivity for HLA-A*01, B*08 have significant positive associations with reports of previous shingles.

  5. 21.2.Schizophrenia

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930403 Family therapy of social psycologic in-tervention and rehabilitation of schizophrenia.KANG Ming(亢明),et al.Dept Psychiatr,3rdPeople’s Hosp,Huaiyin,Jiangsu Prov,223001.Chin J Nerv & Ment Dis 1992;18(5):258—260.Family therapy of social psychologic interven-tion(SPI) was used for the rehabilitation of dis-charged patients with schizophrenia.It was indi-cated that family therapy of SPI had significanteffect on the recovery of social function,im-provement of relation between the patients andmembers of the family,and preventing relapse ofschizophrenia etc.The social function of the

  6. Comment: 212 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available 洋沿岸のマングローブ林に生息する。メダカ近縁種のなかでは唯一の海棲種。 ZZ-ZW型の性決定システムをもつ。 撮影:成瀬清(基礎生物学研究所) Photo: Kiyoshi Naruse (National Institute for Basic Biology) bando 2009/10/01 14:52:41 2010/02/15 15:14:14 ... ...(National Institute for Basic Biology) licensed under CC Attribution2.1 Japan ジャワメダカ (Oryzias javanicus) インド

  7. 212__Omoniyi-LOKOJA

    African Journals Online (AJOL)

    User

    caused by human activities have gradually increased, resulting in the ... has become an environmental concern worldwide due to its toxicity .... soil, hence preventing pollutants from reaching ..... of arsenic, chromium and copper availability to.

  8. EFSA CEF Penal (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2014. Scientific Opinion on Flavouring Group Evaluation 212, Revision 2 (FGE.212Rev2): α,β-Unsaturated alicyclic ketones and precursors from chemical subgroup 2.6 of FGE.19

    DEFF Research Database (Denmark)

    Beltoft, Vibe Meister; Binderup, Mona-Lise; Lund, Pia;

    The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids of the European Food Safety Authority was requested to evaluate the genotoxic potential of 24 flavouring substances from subgroup 2.6 of FGE.19 in the Flavouring Group Evaluation 212, Revision 2. The Panel concluded in ...

  9. Expression and clinical value analysis of plasma miR-205, miR-212 and miR-429 in ovarian cancer%卵巢癌患者血浆miR-205、miR-212及miR-429的水平分析及意义∗

    Institute of Scientific and Technical Information of China (English)

    穆鹏; 李联崑; 贾海清; 王晓彬

    2015-01-01

    Objective To explore the levels of plasma miR⁃205, miR⁃212 and miR⁃429 in ovarian cancer and analyze the re⁃lationship between the above indicators and clinical pathology parameters of ovarian cancer. Methods Plasma samples from 71 ovarian cancer patients before treatment were collected. The real⁃time quantitative RT⁃PCR ( qRT⁃PCR) was used to detect the levels of miR⁃205, miR⁃212 and miR⁃429, and the clinical pathology parameters of ovarian cancer ( age, clinical stage, degree of differentiation, histological type and lymph node metastasis) were collected. The clinical pathology parameters of patients with different levels of miR⁃205, miR⁃212 and miR⁃429 were compared. The receiver operating characteristic curve ( ROC) was employed to analyze the clinical value of plasma miR⁃205, miR⁃212 and miR⁃429 in the diagnosis of ovarian cancer. Meanwhile, the plasma samples from 68 healthy volunteers (healthy control group) and 66 patients with benign ovarian tumor (benign group) were collected as control. Results There were higher levels of miR⁃205 and but lower miR⁃212 and miR⁃429 in ovarian cancer group versus other two groups with signifi⁃cant difference ( P all<0�05) . In ovarian cancer, the level of miR⁃205 was related with age, clinical stage, degree of differentiation and lymph node metastasis, and miR⁃212 was related with clinical stage and degree of differentiation, and miR⁃429 was related with clinical stage and lymph node metastasis ( P all<0�05) . The plasma level of miR⁃205 was negatively correlated with miR⁃212 and miR⁃429 ( r=-0�572、-0�325) , and miR⁃212 was positively correlated with miR⁃429 with statistically significance ( r=0�473,P<0�05) . The AUC, sensitivity and specificity of plasma miR⁃205, miR⁃212 and miR⁃429 in the diagnosis of ovarian cancer were 0�915, 92�1%and 86�2%, 0�944,87�3% and 91�0%, and 0�905, 88�5% and 83�6%. Conclusion There was higher

  10. Study on depression in 212 patients with viral hepatitis%212例慢性病毒性肝炎患者抑郁症状发生的现状分析

    Institute of Scientific and Technical Information of China (English)

    史晓红; 荀健; 王素萍; 张晶

    2009-01-01

    Objective To investigate the symptoms on depression in patients with viral hepatitis. Methods A cross-sectional study was conducted among the patients with viral hepatitis in infectious diseases Hospital of Taiyuan. The questionnaire included a Eysenck Personality Questionnaire, self-rating depression scale (SDS) , and a self-designed one related to information regarding general conditions of the disease and social support. Results (1)Depression symptom prevalence rate among chronic viral hepatitis patients was 54.7% (116/212). (2) Factors as age, occupation, education, confirmed time, number of recurrence and anti-virus treatment, self-confidence on recovery, satisfaction on the surrounding environment etc. that might be associated with depression. (3) The severity of depression was significantly negative correlation with social support scores, objective support scores, subjective support scores (r=-0.262, P=0.000;r=-0.228, P=0.001 ; r=-0.270, P=0.000). (4) There was positive correlation noticed between severity of the depressive disorder and Eysenck Personality two dimensions scores, while the scores of introversion and extroversion scores were negatively correlated (r=-0.330, P=0.000) but positively correlated to the emotional stability scores (r=0.309, P=0.000). Conclusion (1) Patients with hepatitis showed symptoms of depression to a certain degree. (2) Factors as age, occupation, education, economic situation, confirmed time of diagnosis, number of recurrence and anti-virus treatments, confidence on recovery, satisfaction on the surrounding environment might be associated with symptoms of depression. (3) There was positive correlation between severity of depressive and Eysenek Personality two dimensions scores but the scores of introversion and extroversion scores were negatively correlated.%目的 了解慢性病毒性肝炎患者抑郁症状发生的现状.方法 以慢性乙型、丙型肝炎患者作为研究对象,采用问卷调查的方法,用自制

  11. Effects of glucagon-like peptide-1 receptor stimulation and blockade on food consumption and body weight in rats treated with a cannabinoid CB1 receptor agonist WIN 55,212-2.

    Science.gov (United States)

    Radziszewska, Elżbieta; Bojanowska, Ewa

    2013-01-01

    Glucagon-like peptide-1 (GLP-1) and endocannabinoids are involved in appetite control. Recently we have demonstrated that cannabinoid (CB)1 receptor antagonist and GLP-1 receptor agonist synergistically suppress food intake in the rat. The aim of the present study was to determine the effects of GLP-1 receptor stimulation or blockade on feeding behavior in rats treated with WIN 55,212-2, a CB1 receptor agonist. Experiments were performed on adult male Wistar rats. In the first experiment the effects of increasing doses (0.5-4.0 mg/kg) of WIN 55,212-2 injected intraperitoneally on 24-hour food consumption were tested. In further experiments a GLP-1 receptor antagonist, exendin (9-39), and WIN 55,212-2 or a GLP-1 receptor agonist, exendin-4, and WIN 55,212-2 were injected intraperitoneally at subthreshold doses (that alone did not change food intake and body weight) to investigate whether these agents may interact to affect food intake in rats. WIN 55,212-2 administered at low doses (0.5-2 mg/kg) did not markedly change 24-hour food consumption; however, at the highest dose, daily food intake was inhibited. Combined administration of WIN 55,212-2 and exendin (9-39) did not change the amount of food consumed compared to either the control group or to each agent injected alone. Combined injection of WIN 55,212-2 and exendin-4 at subthreshold doses resulted in a significant decrease in food intake and body weight in rats. Stimulation of the peripheral CB1 receptor by its agonist WIN 55,212-2 can induce anorexigenic effects or potentiate, even at a subthreshold dose, the effects of exendin-4, a known anorectic agent. Hence, this dual action of the cannabinoid system should be considered in the medical use of CB1 agonists.

  12. Effects of glucagon-like peptide-1 receptor stimulation and blockade on food consumption and body weight in rats treated with a cannabinoid CB1 receptor agonist WIN 55,212-2

    Science.gov (United States)

    Radziszewska, Elżbieta; Bojanowska, Ewa

    2013-01-01

    Background Glucagon-like peptide-1 (GLP-1) and endocannabinoids are involved in appetite control. Recently we have demonstrated that cannabinoid (CB)1 receptor antagonist and GLP-1 receptor agonist synergistically suppress food intake in the rat. The aim of the present study was to determine the effects of GLP-1 receptor stimulation or blockade on feeding behavior in rats treated with WIN 55,212-2, a CB1 receptor agonist. Material/Methods Experiments were performed on adult male Wistar rats. In the first experiment the effects of increasing doses (0.5–4.0 mg/kg) of WIN 55,212-2 injected intraperitoneally on 24-hour food consumption were tested. In further experiments a GLP-1 receptor antagonist, exendin (9-39), and WIN 55,212-2 or a GLP-1 receptor agonist, exendin-4, and WIN 55,212-2 were injected intraperitoneally at subthreshold doses (that alone did not change food intake and body weight) to investigate whether these agents may interact to affect food intake in rats. Results WIN 55,212-2 administered at low doses (0.5–2 mg/kg) did not markedly change 24-hour food consumption; however, at the highest dose, daily food intake was inhibited. Combined administration of WIN 55,212-2 and exendin (9-39) did not change the amount of food consumed compared to either the control group or to each agent injected alone. Combined injection of WIN 55,212-2 and exendin-4 at subthreshold doses resulted in a significant decrease in food intake and body weight in rats. Conclusions Stimulation of the peripheral CB1 receptor by its agonist WIN 55,212-2 can induce anorexigenic effects or potentiate, even at a subthreshold dose, the effects of exendin-4, a known anorectic agent. Hence, this dual action of the cannabinoid system should be considered in the medical use of CB1 agonists. PMID:23291632

  13. Consequences of Adolescent Exposure to the Cannabinoid Receptor Agonist WIN55,212-2 on Working Memory in Female Rats

    Directory of Open Access Journals (Sweden)

    Erin K. Kirschmann

    2017-07-01

    Full Text Available Marijuana is a prevalent illicit substance used by adolescents, and several studies have indicated that adolescent use can lead to long-term cognitive deficits including problems with attention and memory. However, preclinical animal studies that observe cognitive deficits after cannabinoid exposure during adolescence utilize experimenter administration of doses of cannabinoids that may exceed what an organism would choose to take, suggesting that contingency and dose are critical factors that need to be addressed in translational models of consequences of cannabinoid exposure. Indeed, we recently developed an adolescent cannabinoid self-administration paradigm in male rats, and found that prior adolescent self-administration of the cannabinoid receptor agonist WIN55,212-2 (WIN resulted in improved working memory performance in adulthood. In addition, the doses self-administered were not as high as those that are found to produce memory deficits. However, given known sex differences in both drug self-administration and learning and memory processes, it is possible that cannabinoid self-administration could have different cognitive consequences in females. Therefore, we aimed to explore the effects of self-administered vs. experimenter-administered WIN in adolescent female rats on adult cognitive function. Female rats were trained to self-administer WIN daily throughout adolescence (postnatal day 34–59. A control group self-administered vehicle solution. The acute effects of adolescent WIN self-administration on memory were determined using a short-term spatial memory test 24 h after final SA session; and the long-term effects on cognitive performance were assessed during protracted abstinence in adulthood using a delayed-match-to-sample working memory task. In a separate experiment, females were given daily intraperitoneal (IP injections of a low or high dose of WIN, corresponding to self-administered and typical experimenter

  14. Polymorphisms in a Putative Enhancer at the 10q21.2 Breast Cancer Risk Locus Regulate NRBF2 Expression

    Science.gov (United States)

    Darabi, Hatef; McCue, Karen; Beesley, Jonathan; Michailidou, Kyriaki; Nord, Silje; Kar, Siddhartha; Humphreys, Keith; Thompson, Deborah; Ghoussaini, Maya; Bolla, Manjeet K.; Dennis, Joe; Wang, Qin; Canisius, Sander; Scott, Christopher G.; Apicella, Carmel; Hopper, John L.; Southey, Melissa C.; Stone, Jennifer; Broeks, Annegien; Schmidt, Marjanka K.; Scott, Rodney J.; Lophatananon, Artitaya; Muir, Kenneth; Beckmann, Matthias W.; Ekici, Arif B.; Fasching, Peter A.; Heusinger, Katharina; dos-Santos-Silva, Isabel; Peto, Julian; Tomlinson, Ian; Sawyer, Elinor J.; Burwinkel, Barbara; Marme, Frederik; Guénel, Pascal; Truong, Thérèse; Bojesen, Stig E.; Flyger, Henrik; Benitez, Javier; González-Neira, Anna; Anton-Culver, Hoda; Neuhausen, Susan L.; Arndt, Volker; Brenner, Hermann; Engel, Christoph; Meindl, Alfons; Schmutzler, Rita K.; Arnold, Norbert; Brauch, Hiltrud; Hamann, Ute; Chang-Claude, Jenny; Khan, Sofia; Nevanlinna, Heli; Ito, Hidemi; Matsuo, Keitaro; Bogdanova, Natalia V.; Dörk, Thilo; Lindblom, Annika; Margolin, Sara; Kosma, Veli-Matti; Mannermaa, Arto; Tseng, Chiu-chen; Wu, Anna H.; Floris, Giuseppe; Lambrechts, Diether; Rudolph, Anja; Peterlongo, Paolo; Radice, Paolo; Couch, Fergus J.; Vachon, Celine; Giles, Graham G.; McLean, Catriona; Milne, Roger L.; Dugué, Pierre-Antoine; Haiman, Christopher A.; Maskarinec, Gertraud; Woolcott, Christy; Henderson, Brian E.; Goldberg, Mark S.; Simard, Jacques; Teo, Soo H.; Mariapun, Shivaani; Helland, Åslaug; Haakensen, Vilde; Zheng, Wei; Beeghly-Fadiel, Alicia; Tamimi, Rulla; Jukkola-Vuorinen, Arja; Winqvist, Robert; Andrulis, Irene L.; Knight, Julia A.; Devilee, Peter; Tollenaar, Robert A.E.M.; Figueroa, Jonine; García-Closas, Montserrat; Czene, Kamila; Hooning, Maartje J.; Tilanus-Linthorst, Madeleine; Li, Jingmei; Gao, Yu-Tang; Shu, Xiao-Ou; Cox, Angela; Cross, Simon S.; Luben, Robert; Khaw, Kay-Tee; Choi, Ji-Yeob; Kang, Daehee; Hartman, Mikael; Lim, Wei Yen; Kabisch, Maria; Torres, Diana; Jakubowska, Anna; Lubinski, Jan; McKay, James; Sangrajrang, Suleeporn; Toland, Amanda E.; Yannoukakos, Drakoulis; Shen, Chen-Yang; Yu, Jyh-Cherng; Ziogas, Argyrios; Schoemaker, Minouk J.; Swerdlow, Anthony; Borresen-Dale, Anne-Lise; Kristensen, Vessela; French, Juliet D.; Edwards, Stacey L.; Dunning, Alison M.; Easton, Douglas F.; Hall, Per; Chenevix-Trench, Georgia

    2015-01-01

    Genome-wide association studies have identified SNPs near ZNF365 at 10q21.2 that are associated with both breast cancer risk and mammographic density. To identify the most likely causal SNPs, we fine mapped the association signal by genotyping 428 SNPs across the region in 89,050 European and 12,893 Asian case and control subjects from the Breast Cancer Association Consortium. We identified four independent sets of correlated, highly trait-associated variants (iCHAVs), three of which were located within ZNF365. The most strongly risk-associated SNP, rs10995201 in iCHAV1, showed clear evidence of association with both estrogen receptor (ER)-positive (OR = 0.85 [0.82–0.88]) and ER-negative (OR = 0.87 [0.82–0.91]) disease, and was also the SNP most strongly associated with percent mammographic density. iCHAV2 (lead SNP, chr10: 64,258,684:D) and iCHAV3 (lead SNP, rs7922449) were also associated with ER-positive (OR = 0.93 [0.91–0.95] and OR = 1.06 [1.03–1.09]) and ER-negative (OR = 0.95 [0.91–0.98] and OR = 1.08 [1.04–1.13]) disease. There was weaker evidence for iCHAV4, located 5′ of ADO, associated only with ER-positive breast cancer (OR = 0.93 [0.90–0.96]). We found 12, 17, 18, and 2 candidate causal SNPs for breast cancer in iCHAVs 1–4, respectively. Chromosome conformation capture analysis showed that iCHAV2 interacts with the ZNF365 and NRBF2 (more than 600 kb away) promoters in normal and cancerous breast epithelial cells. Luciferase assays did not identify SNPs that affect transactivation of ZNF365, but identified a protective haplotype in iCHAV2, associated with silencing of the NRBF2 promoter, implicating this gene in the etiology of breast cancer. PMID:26073781

  15. 3种微创手术治疗复杂性输尿管上段结石212例比较%A comparison of three surgical procedures of complicated upper ureteral calculi for 212 cases

    Institute of Scientific and Technical Information of China (English)

    李旭明; 程洪林; 郭闯; 程宗勇; 李锋; 张力; 李元仲

    2011-01-01

    Objective To compare the efficacy of three procedures, ureteroscopic litripsy(URL), minimally invasive percutaneous nephrolithotomy(MPCNL) ,and retroperitoneal laparoscopic ureterolithomy(RLU), for the treatment of complicated upper ureteral calculi. Methods Two hundred and thirteen cases of complicated upper ureteral calculi were treated in this hospital from February 2002 to February 2010,including fifty-two cases of URL, one hundred and seven cases of MPCNL, and fifty-four cases of RLU. Results The stone free rate seven days after operation:URL was 36.5%(19/52),MPCNL was 75.7%(81/107),RLU was 90.6% (48/53); the stone free rate one months after operation: URL was 71. 1% (38/52), MPCNL was 95. 3% (102/107), RLU was 96.2%(51/53); the stone-free rate three months after operation:URL was 82.7%(43/52),MPCNL was 98. 1%(105/107),RLU was 98.1%(52/53). There was significantly difference between URL and MPCNL(P<0.05) ,URL and RLU (P<0.05). There was also significantly difference between MPCNL and RLU in the near future(P<0. 05) ,but had no difference in long term. Conclusion High stone free rate can be obtained with RLU and MPCNL for impacted upper ureteral calculi. The stone-free rate is less satisfactory with URL.%目的 比较输尿管镜碎石(URL)、微创经皮肾镜取石(MPCNL)、后腹腔镜下输尿管切开取石(RLU)治疗复杂性输尿管上段结石的疗效.方法 回顾分析2002年2月至2010年2月复杂性输尿管上段结石212例,其中URL治疗52例,MPCNL治疗107例,RLU治疗54例.比较分析3种手术的治疗效果.结果 术后7 d结石清除率:URL组36.5%(19/52),MPCNL组75.7%(81/107),RLU组90.6%(48/53).术后1个月结石清除率:URL组73.1%(38/52),MPCNL组95.3%(102/107),RLU组96.2%(51/53).术后3个月结石清除率:URL组82.7%(43/52),MPCNL组98.1%(105/107),RLU组98.1%(52/53).URL组分别与MPCNL组、RLU组比较差异有统计学意义(P<0.05),MPCNL、RLU组之间比较近期差异有统计学意义(P<0.05),

  16. The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells.

    Science.gov (United States)

    Wilhelmsen, Kevin; Khakpour, Samira; Tran, Alphonso; Sheehan, Kayla; Schumacher, Mark; Xu, Fengyun; Hellman, Judith

    2014-05-09

    Although cannabinoids, such as Δ(9)-tetrahydrocannabinol, have been studied extensively for their psychoactive effects, it has become apparent that certain cannabinoids possess immunomodulatory activity. Endothelial cells (ECs) are centrally involved in the pathogenesis of organ injury in acute inflammatory disorders, such as sepsis, because they express cytokines and chemokines, which facilitate the trafficking of leukocytes to organs, and they modulate vascular barrier function. In this study, we find that primary human ECs from multiple organs express the cannabinoid receptors CB1R, GPR18, and GPR55, as well as the ion channel transient receptor potential cation channel vanilloid type 1. In contrast to leukocytes, CB2R is only minimally expressed in some EC populations. Furthermore, we show that ECs express all of the known endocannabinoid (eCB) metabolic enzymes. Examining a panel of cannabinoids, we demonstrate that the synthetic cannabinoid WIN55,212-2 and the eCB N-arachidonoyl dopamine (NADA), but neither anandamide nor 2-arachidonoylglycerol, reduce EC inflammatory responses induced by bacterial lipopeptide, LPS, and TNFα. We find that endothelial CB1R/CB2R are necessary for the effects of NADA, but not those of WIN55,212-2. Furthermore, transient receptor potential cation channel vanilloid type 1 appears to counter the anti-inflammatory properties of WIN55,212-2 and NADA, but conversely, in the absence of these cannabinoids, its inhibition exacerbates the inflammatory response in ECs activated with LPS. These data indicate that the eCB system can modulate inflammatory activation of the endothelium and may have important implications for a variety of acute inflammatory disorders that are characterized by EC activation.

  17. The Endocannabinoid/Endovanilloid N-Arachidonoyl Dopamine (NADA) and Synthetic Cannabinoid WIN55,212-2 Abate the Inflammatory Activation of Human Endothelial Cells*

    Science.gov (United States)

    Wilhelmsen, Kevin; Khakpour, Samira; Tran, Alphonso; Sheehan, Kayla; Schumacher, Mark; Xu, Fengyun; Hellman, Judith

    2014-01-01

    Although cannabinoids, such as Δ9-tetrahydrocannabinol, have been studied extensively for their psychoactive effects, it has become apparent that certain cannabinoids possess immunomodulatory activity. Endothelial cells (ECs) are centrally involved in the pathogenesis of organ injury in acute inflammatory disorders, such as sepsis, because they express cytokines and chemokines, which facilitate the trafficking of leukocytes to organs, and they modulate vascular barrier function. In this study, we find that primary human ECs from multiple organs express the cannabinoid receptors CB1R, GPR18, and GPR55, as well as the ion channel transient receptor potential cation channel vanilloid type 1. In contrast to leukocytes, CB2R is only minimally expressed in some EC populations. Furthermore, we show that ECs express all of the known endocannabinoid (eCB) metabolic enzymes. Examining a panel of cannabinoids, we demonstrate that the synthetic cannabinoid WIN55,212-2 and the eCB N-arachidonoyl dopamine (NADA), but neither anandamide nor 2-arachidonoylglycerol, reduce EC inflammatory responses induced by bacterial lipopeptide, LPS, and TNFα. We find that endothelial CB1R/CB2R are necessary for the effects of NADA, but not those of WIN55,212-2. Furthermore, transient receptor potential cation channel vanilloid type 1 appears to counter the anti-inflammatory properties of WIN55,212-2 and NADA, but conversely, in the absence of these cannabinoids, its inhibition exacerbates the inflammatory response in ECs activated with LPS. These data indicate that the eCB system can modulate inflammatory activation of the endothelium and may have important implications for a variety of acute inflammatory disorders that are characterized by EC activation. PMID:24644287

  18. Radioimmunoscintigraphy of colorectal carcinoma using technetium-99m-labeled, totally human monoclonal antibody 88BV59H21-2.

    Science.gov (United States)

    Gulec, S A; Serafini, A N; Moffat, F L; Vargas-Cuba, R D; Sfakianakis, G N; Franceschi, D; Crichton, V Z; Subramanian, R; Klein, J L; De Jager, R L

    1995-12-01

    Radioimmunoscintigraphy (RIS) using human monoclonal antibodies offers the important clinical advantage of repeated imaging over murine monoclonal antibodies by eliminating the cross-species antibody response. This article reports a Phase I-II clinical trial with Tc-99m-labeled, totally human monoclonal antibody 88BV59H21-2 in patients with colorectal carcinoma. The study population consisted of 34 patients with colorectal cancer (20 men and 14 women; age range, 44-81 years). Patients were administered 5-10 mg antibody labeled with 21-41 mCi Tc-99m by the i.v. route and imaged at 3-10 and 16-24 h after infusion using planar and single-photon emission computed tomographic (CT) techniques. Pathological confirmation was obtained in 25 patients who underwent surgery. Human antihuman antibody (HAHA) titers were checked prior to and 1 and 3 months after the infusion. RIS with Tc-99m-labeled 88BV59H21-2 revealed a better detection rate in the abdomen-pelvis region compared with axial CT. The combined use of both modalities increased the sensitivity in both the liver and abdomen-pelvis regions. Ten patients developed mild adverse reactions (chills and fever). No HAHA response was detected in this series. Tc-99m-labeled human monoclonal antibody 88BV59H21-2 RIS shows promise as a useful diagnostic modality in patients with colorectal cancer. RIS alone or in combination with CT is more sensitive than CT in detecting tumor within the abdomen and pelvis. Repeated RIS studies may be possible, due to the lack of a HAHA response.

  19. Uv-visible spectroscopy of bacteriorhodopsin mutants: substitution of Arg-82, Asp-85, Tyr-185, and Asp-212 results in abnormal light-dark adaptation.

    Science.gov (United States)

    Duñach, M; Marti, T; Khorana, H G; Rothschild, K J

    1990-12-01

    The light-dark adaptation reactions of a set of bacteriorhodopsin (bR) mutants that affect function and color of the chromophore were examined by using visible absorption spectroscopy. The absorbance spectra of the mutants Arg-82 in equilibrium Ala (Gln), Asp-85 in equilibrium Ala (Asn, Glu), Tyr-185 in equilibrium Phe, and Asp-212 in equilibrium Ala (Asn, Glu) were measured at different pH values during and after illumination. None of these mutants exhibited a normal dark-light adaptation, which in wild-type bR causes a red shift of the visible absorption maximum from 558 nm (dark-adapted bR) to 568 nm (light-adapted bR). Instead a reversible light reaction occurs in the Asp-85 and Asp-212 mutants from a blue form with lambda max near 600 nm to a pink form with lambda max near 480 nm. This light-induced shift explains the appearance of a reversed light adaptation previously observed for the Asp-212 mutants. In the case of the Tyr-185 and Arg-82 mutants, light causes a purple-to-blue transformation similar to the effect of lowering the pH. However, the blue forms observed in these mutants are not identical to those formed by acid titration or deionization of wild-type bR. It is suggested that in all of these mutants, the chromophore has lost the ability to undergo the normal 13-cis, 15-syn to all-trans, 15-anti light-driven isomerization, which occurs in native bR. Instead these mutants may have as stable forms all-trans,syn and 13-cis,anti chromophores, which are not allowed in native bR, except transiently.

  20. Étude des dommages induits dans l'ADN par irradiation laser X-UV à 21.2 nm

    Science.gov (United States)

    Cassou, K.; Ros, D.; Kazamias, S.; Klisnick, A.; Jamelot, G.; Guilbaud, O.; Rus, B.; Kozlová, M.; Polan, J.; Präg, A. R.; Stupka, M.; Eot-Houllier, G.; Sage, E.; Begusová, M.; Stísová, V.; Du Penhoat, M.-A. H.; Touati, A.; Chétioui, A.

    2005-06-01

    We report the preliminary result of the application of the collisional Ne-like soft x-ray laser as radiation source to induce DNA damage. The goal of this experiment was a test bed study of the damage yields induced by soft x-ray radiation in dried plasmid DNA sample. The saturated Ne-like soft x-ray laser available at the PALS facility, delivering several millijoules in a single 100 ps pulse at 21.2 nm was used to irradiate two different plasmids: pSP189 and pBS. The study is centered on the dose effect leading to single and double strand break in DNA.

  1. Cherenkov light production from the α-emitting decay chains of (223)Ra, (212)Pb, and (149)Tb for Cherenkov Luminescence Imaging.

    Science.gov (United States)

    Wood, V; Ackerman, N L

    2016-12-01

    Cherenkov Luminescence Imaging (CLI) is a new method to image radioactive therapeutic and diagnostic agents, primarily in preclinical studies. This study used Geant4 and Python to generate the predicted Cherenkov light production as a function of time for a set of isotopic chains of interest for targeted alpha therapy: (223)Ra, (212)Pb, and (149)Tb. All are shown to produce substantial Cherenkov light, though time delays between initial decays and the production of Cherenkov light requires caution in interpreting CLI. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  2. Measuring Progress on the Control of Porcine Reproductive and Respiratory Syndrome (PRRS at a Regional Level: The Minnesota N212 Regional Control Project (Rcp as a Working Example.

    Directory of Open Access Journals (Sweden)

    Pablo Valdes-Donoso

    Full Text Available Due to the highly transmissible nature of porcine reproductive and respiratory syndrome (PRRS, implementation of regional programs to control the disease may be critical. Because PRRS is not reported in the US, numerous voluntary regional control projects (RCPs have been established. However, the effect of RCPs on PRRS control has not been assessed yet. This study aims to quantify the extent to which RCPs contribute to PRRS control by proposing a methodological framework to evaluate the progress of RCPs. Information collected between July 2012 and June 2015 from the Minnesota Voluntary Regional PRRS Elimination Project (RCP-N212 was used. Demography of premises (e.g. composition of farms with sows = SS and without sows = NSS was assessed by a repeated analysis of variance. By using general linear mixed-effects models, active participation of farms enrolled in the RCP-N212, defined as the decision to share (or not to share PRRS status, was evaluated and used as a predictor, along with other variables, to assess the PRRS trend over time. Additionally, spatial and temporal patterns of farmers' participation and the disease dynamics were investigated. The number of farms enrolled in RCP-N212 and its geographical coverage increased, but the proportion of SS and NSS did not vary significantly over time. A significant increasing (p<0.001 trend in farmers' decision to share PRRS status was observed, but with NSS producers less willing to report and a large variability between counties. The incidence of PRRS significantly (p<0.001 decreased, showing a negative correlation between degree of participation and occurrence of PRRS (p<0.001 and a positive correlation with farm density at the county level (p = 0.02. Despite a noted decrease in PRRS, significant spatio-temporal patterns of incidence of the disease over 3-weeks and 3-kms during the entire study period were identified. This study established a systematic approach to quantify the effect of RCPs on

  3. An M12(L(1))12(L(2))12 cantellated tetrahedron: a case study on mixed-ligand self-assembly.

    Science.gov (United States)

    Sun, Qing-Fu; Sato, Sota; Fujita, Makoto

    2014-12-01

    In the self-assembly of Pd(II) ions and two different, but similarly shaped, ligands (1 and 2), neither random mixing nor self-sorting of the two ligands into two unmixed structures was observed. Instead a mixed, yet sorted, Pd12 (1)12 (2)12 cantellated tetrahedron (and its pseudoisomer) was selectively formed, thus revealing a fine example of intramolecular self-sorting. A case study showed that a homothetic ratio of >2 is necessary to observe cantellated tetrahedra.

  4. Double partial trisomy of 6p23-pter and 9pter-q21.2 in a neonate resulting from 4:2 meiotic segregation of a maternal complex t(6;7;9)(p23;p15;q21.2) translocation.

    Science.gov (United States)

    Cetin, Z; Mihci, E; Keser, I; Karaali, K; Berker, S; Luleci, G

    2012-01-01

    We report, a newborn presenting multiple congenital abnormalities with karyotype; 47,XY,der(7)t(6;7)(pter-p23::p15-->qter),+der(9)t(7;9)(pter-->p15::q21.2--> pter)t(6;7;9)(p23;p15;q21.2)mat[20]. The mother and her phenotypically normal daughter were carriers of a complex chromosomal rearrangement with karyotypes; 46,XX,t(6;7;9)(p23;p15;q21.2)[20]. Paternal chromosomes were normal. In our case the extra derivative chromosome was the result of a 4:2 segregation of the chromosomes involved in translocation during oogenesis. Double partial trisomy in newborns resulting from 4:2 segregation is a rare event, and double partial trisomies of the 6p23-pter and trisomy 9pter-q22 regions have not reported to date.

  5. PdBI sub-arcsecond study of the SiO microjet in HH212 - Origin and collimation of Class 0 jets

    CERN Document Server

    Cabrit, S; Gueth, F; Nisini, B; Gusdorf, A; Dougados, C; Bacciotti, F

    2007-01-01

    The bipolar HH 212 outflow has been mapped in SiO using the extended configuration of the Plateau de Bure Interferometer (PdBI), revealing a highly collimated SiO jet closely associated with the H2 jet component. We study at unprecedented resolution (0.34" across the jet axis) the properties of the innermost SiO ``microjet'' within 1000 AU of this young Class 0 source, to compare it with atomic microjets from more evolved sources and to constrain its origin. The SiO channel maps are used to investigate the microjet collimation and velocity structure. A large velocity gradient analysis is applied to SiO (2-1), (5-4) and (8-7) data from the PdBI and the Submillimeter Array to constrain the SiO opacity and abundance. The HH212 Class 0 microjet shows striking similarities in collimation and energetic budget with atomic microjets from T Tauri sources. Furthermore, the SiO lines appear optically thick, unlike what is generally assumed. We infer T(kin) ~ 50-500 K and an SiO/H2 abundance greater than 4 10(-8)-6 10(-5...

  6. ASUPAN KALSIUM DAN VITAMIN D PADA ANAK INDONESIA USIA 2 – 12 TAHUN [Calcium and Vitamin D Intake of Indonesian Children 2-12 Years Old

    Directory of Open Access Journals (Sweden)

    Victoria Valentina*

    2014-09-01

    Full Text Available Food consumption is playing an important role for nutritional status of children 2-12 years old. During growth and development phase, body needs macro and micro nutrients even more than later phase. The process can not be catched up at later stage therefore it is very important to pay attention to food consumption during this phase. This study used secondary data of South East Asian Nutrition Surveys (SEANUTS 2011-2012 which was covered recapitulation profile data of 300 children age 2-12 years old representative of 48 districts in Indonesia. The study showed that Indonesian children consuming less calcium and vitamin D-rich foods than the recommended daily allowance (RDA. It does not reflects on the result of anthropometry data, vitamin D on the blood and bone mass density since the dietary recall 24 hours shows only food consumption in that specific day while nutritional status shows a result of longer process. However, vitamin D in blood has a significant correlation with bone mass density of tibia bone (p<0.05. Deficiency of calcium and mainly vitamin D is a new finding at this time. Therefore, the study showed that fortification of calcium and vitamin min D in food become important to improve nutritional status of Indonesian children. 20% of RDA per serving size twice a day is recommended as well for fortification level of Calcium and Vitamin D.

  7. Vibrational spectroscopy of bacteriorhodopsin mutants: light-driven proton transport involves protonation changes of aspartic acid residues 85, 96, and 212

    Energy Technology Data Exchange (ETDEWEB)

    Braiman, M.S.; Mogi, T.; Marti, T.; Stern, L.J.; Khorana, H.G.; Rothschild, K.J.

    1988-11-15

    Fourier transform infrared (FTIR) difference spectra have been obtained for the bR----K, bR----L, and bR----M photoreactions in bacteriorhodopsin mutants in which Asp residues 85, 96, 115, and 212 have been replaced by Asn and by Glu. Difference peaks that had previously been attributed to Asp COOH groups on the basis of isotopic labeling were absent or shifted in these mutants. In general, each COOH peak was affected strongly by mutation at only one of the four residues. Thus, it was possible to assign each peak tentatively to a particular Asp. From these assignments, a model for the proton-pumping mechanism of bR is derived, which features proton transfers among Asp-85, -96, and -212, the chromophore Schiff base, and other ionizable groups within the protein. The model can explain the observed COOH peaks in the FTIR difference spectra of bR photointermediates and could also account for other recent results on site-directed mutants of bR.

  8. 13CO2/12CO2 ratio analysis in exhaled air by lead-salt tunable diode lasers for noninvasive diagnostics in gastroenterology

    Science.gov (United States)

    Stepanov, Eugene V.; Zyrianov, Pavel V.; Miliaev, Valerii A.; Selivanov, Yurii G.; Chizhevskii, Eugene G.; Os'kina, Svetlana; Ivashkin, Vladimir T.; Nikitina, Elena I.

    1999-07-01

    An analyzer of 13CO2/12CO2 ratio in exhaled air based on lead-salt tunable diode lasers is presented. High accuracy of the carbon isotope ratio detection in exhaled carbon dioxide was achieved with help of very simple optical schematics. It was based on the use of MBE laser diodes operating in pulse mode and on recording the resonance CO2 absorption at 4.2 micrometers . Special fast acquisition electronics and software were applied for spectral data collection and processing. Developed laser system was tested in a clinical train aimed to assessment eradication efficiency in therapy of gastritis associated with Helicobacter pylori infection. Data on the 13C-urea breath test used for P.pylori detection and obtained with tunable diode lasers in the course of the trail was compared with the results of Mass-Spectroscopy analysis and histology observations. The analyzer can be used also for 13CO2/12CO2 ratio detection in exhalation to perform gastroenterology breath test based on using other compounds labeled with stable isotopes.

  9. Electrostatics of the photosynthetic bacterial reaction center. Protonation of Glu L 212 and Asp L 213 - A new method of calculation.

    Science.gov (United States)

    Ptushenko, Vasily V; Cherepanov, Dmitry A; Krishtalik, Lev I

    2015-12-01

    Continuum electrostatic calculation of the transfer energies of anions from water into aprotic solvents gives the figures erroneous by order of magnitude. This is due to the hydrogen bond disruption that suggests the necessity to reconsider the traditional approach of the purely electrostatic calculation of the transfer energy from water into protein. In this paper, the method combining the experimental estimates of the transfer energies from water into aprotic solvent and the electrostatic calculation of the transfer energies from aprotic solvent into protein is proposed. Hydrogen bonds between aprotic solvent and solute are taken into account by introducing an imaginary aprotic medium incapable to form hydrogen bonds with the solute. Besides, a new treatment of the heterogeneous intraprotein dielectric permittivity based on the microscopic protein structure and electrometric measurements is elaborated. The method accounts semi-quantitatively for the electrostatic effect of diverse charged amino acid substitutions in the donor and acceptor parts of the photosynthetic bacterial reaction center from Rhodobacter sphaeroides. Analysis of the volatile secondary acceptor site QB revealed that in the conformation with a minimal distance between quinone QB and Glu L 212 the proton uptake upon the reduction of QB is prompted by Glu L 212 in alkaline and by Asp L 213 in slightly acidic regions. This agrees with the pH dependences of protonation degrees and the proton uptake. The method of pK calculation was applied successfully also for dissociation of Asp 26 in bacterial thioredoxin.

  10. Study of miRNA-212 regulating BDNF/TrkB signaling pathway in epileptic neuron model%神经元癫(癎)模型miRNA-212对BDNF/TrkB信号转导通路影响的研究

    Institute of Scientific and Technical Information of China (English)

    蔡浩; 吴秋静; 朱延霞; 赵文; 李斌; 宋毅军

    2014-01-01

    目的 探讨海马神经元癫(癎)模型转染微小RNA(miRNA)-212对脑源性神经营养因子(BDNF)/酪氨酸蛋白激酶B(TrkB)信号转导通路的影响.方法 经体外培养7d的大鼠海马神经元(正常对照组),通过无镁离子细胞外液处理(3 h)方法建立癫(癎)神经元模型(癫(癎)模型组),继而以含镁离子正常细胞外液与慢病毒稀释液转染构建miRNA-212慢病毒表达载体;免疫荧光双染法、膜片钳技术和免疫印迹法观察转染miRNA-212对BDNF/TrkB信号转导通路的影响.结果 无论是正常海马神经元或癫痴神经元模型,与BDNF共培养后其磷酸化TrkB (pTrkB)/TrkB比值均显著升高(P=0.001),表明BDNF/TrkB信号转导通路被激活;转染miRNA-212后,癫(癎)神经元模型pTrkB/TrkB比值降低并低于未转染者(P=0.001),提示BDNF/TrkB信号转导通路受到抑制.结论 BDNF具有激活海马神经元BDNF/TrkB信号转导通路作用,而转染miRNA-212可抑制BDNF/TrkB信号转导通路.

  11. 大麻素受体激动剂WIN55,212-2预处理对大鼠脊髓缺血再灌注损伤的保护作用研究%Protective effects of cannabinoid receptor agonist WIN55,212-2 preconditioning on spinal cord ischemia reperfusion injury in rats

    Institute of Scientific and Technical Information of China (English)

    荆娜; 马虹

    2015-01-01

    目的 探讨大麻素受体激动剂WIN55,212-2预处理对大鼠脊髓缺血再灌注损伤的保护作用.方法 32只SD大鼠按随机数字表法分为4组(n=8),分别为Sham组(假手术组)、Control组(单纯缺血对照组)、二甲基亚砜(DMSO)组(溶剂组)、WIN组(WIN55,212-2预处理组).Control组在诱导脊髓缺血前30 min腹腔内注射生理盐水0.3ml,DMSO组在诱导脊髓缺血前30 min腹腔内注射DMSO 0.3 ml,WIN组在诱导脊髓缺血前30 min腹腔内注射WIN55,212-2 1 mg/kg.分别于再灌注24、48 h采用Tarlov评分标准对后肢运动功能进行评价,之后取L4~L6脊髓组织行病理学检查.结果 再灌注24h和48 h,缺血组Tarlov评分均低于Sham组(P<0.05),WIN组Tarlov评分明显高于Control组和DMSO组(P<0.05).缺血组前角正常神经元数量少于Sham组(P<0.05),WIN组明显多于Control组和DMSO组(P<0.05).结论 大麻素受体激动剂WIN55,212-2预处理能够减轻大鼠脊髓缺血再灌注损伤.%Objective To explore the effects of cannabinoid receptor agonist WIN55,212-2 preconditioning on spinal cord ischemia reperfusion injury in rats.Methods A total of 32 male Sprague-Dawley rats was randomly divided into four groups (n =8):sham group,control group,dimethyl sulfoxide(DMSO) group which was given intraperitoneally DMSO 0.3 ml 30 min before ischemia reperfusion,and WIN group which was given intraperitoneally WIN55,212-2 1 mg/kg 30 min before ischemia reperfusion.Each rat was neurologically assessed at 24 h and 48 h after reperfusion by Tarlov scale,and the number of normal motor neurons at anterior horn of the spinal cord was recorded.Res uits The Tarlov scale of WIN group was significantly higher than that control and DMSO groups (P < 0.05).There were more normal motor neurons at anterior horn of the spinal cord in WIN group than those in control and DMSO groups (P < 0.05).Conclusions Cannabinoid receptor agonist WIN55,212-2 preconditioning might attenuate spinal cord ischemia

  12. Tyrosine 105 and threonine 212 at outermost substrate binding subsites -6 and +4 control substrate specificity, oligosaccharide cleavage patterns, and multiple binding modes of barley alpha-amylase 1

    DEFF Research Database (Denmark)

    Bak-Jensen, K.S.; André, G.; Gottschalk, T.E.

    2004-01-01

    The role in activity of outer regions in the substrate binding cleft in alpha-amylases is illustrated by mutational analysis of Tyr(105) and Thr(212) localized at subsites - 6 and +4 ( substrate cleavage occurs between subsites -1 and +1) in barley alpha-amylase 1 (AMY1). Tyr(105) is conserved...... in plant alpha-amylases whereas Thr(212) varies in these and related enzymes. Compared with wild-type AMY1, the subsite -6 mutant Y105A has 140, 15, and ...% activity, respectively. Thus engineering of aromatic stacking interactions at the ends of the 10-subsite long binding cleft affects activity very differently, dependent on the substrate. Y105A dominates in dual subsite -6/+4 [Y105A/T212(Y/W)] AMY1 mutants having almost retained and low activity on starch...

  13. WIN55, 212-2 promotes differentiation of oligodendrocyte precursor cells and improve remyelination through regulation of the phosphorylation level of the ERK 1/2 via cannabinoid receptor 1 after stroke-induced demyelination.

    Science.gov (United States)

    Sun, Jing; Fang, Yinquan; Chen, Tao; Guo, Jingjing; Yan, Jun; Song, Shu; Zhang, Luyong; Liao, Hong

    2013-01-23

    In stroke, a common cause of neurological disability in adults is that the myelin sheaths are lost through the injury or death of mature oligodendrocytes, and the failure of remyelination may be often due to insufficient proliferation and differentiation of oligodendroglial progenitors. In the current study, we used middle cerebral artery occlusion (MCAO) to induced transient focal cerebral ischemia, and found that WIN55, 212-2 augmented actively proliferating oligodendrocytes measured by CC1 immunoreactive cells within the peri-infarct areas. To establish whether these effects were associated with changes in myelin formation, we analyzed the expression of myelin basic protein (MBP) and myelin ultrastructure. We found that WIN55, 212-2 showed more extensive remyelination than vehicle at 14 days post injection (dpi). The extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling pathway may be involved in OPCs differentiation. To determine the regulatory effect of WIN55, 212-2 post-treatment on phospho-ERK 1/2 (p-ERK 1/2) after ischemia/reperfusion, Western blot analysis was performed. We found that WIN55, 212-2 regulated the phosphorylation level of the ERK 1/2 to promote OPCs survival and differentiation. Notably, cannabinoid receptor 1 is coupled to the activation of the ERK cascade. Following rimonabant combined treatment, the effect of WIN55, 212-2 on regulating the phosphorylation level of the ERK 1/2 was reversed, and the effect of accelerated myelin formation was partially inhibited. Together, we first found that WIN55, 212-2 promoted OPCs differentiation and remyelination through regulation of the level of the p-ERK 1/2 via cannabinoid receptor 1.

  14. Inherited 1q21.1q21.2 duplication and 16p11.2 deletion: a two-hit case with more severe clinical manifestations.

    Science.gov (United States)

    Brisset, Sophie; Capri, Yline; Briand-Suleau, Audrey; Tosca, Lucie; Gras, Domitille; Fauret-Amsellem, Anne-Laure; Pineau, Dominique; Saada, Julien; Ortonne, Valérie; Verloes, Alain; Goossens, Michel; Tachdjian, Gérard; Métay, Corinne

    2015-09-01

    We report paternally inherited duplication of 1q12q21.2 of 5.8 Mb associated with maternally inherited deletion of 16p11.2 of 545 Kb, this latter first identified in a fetus exhibiting an absent nasal bone detected during pregnancy. During the neonatal period, the young boy presented developmental delay, epilepsy, congenital anomalies and overweight. The clinical features of the proband with two rearrangements were more severe than in either of the parents carrying only one or the other mutation. Thus our data support a two-hit model in which the concomitant presence of these two copy-number variations exacerbates the neurodevelopmental phenotype.

  15. Geometric and electronic structures of (BeO)(N) (N = 2-12, 16, 20, and 24): rings, double rings, and cages.

    Science.gov (United States)

    Ren, Lei; Cheng, Longjiu; Feng, Yan; Wang, Xuemei

    2012-07-07

    The structure of (BeO)(N) clusters (N = 2-12, 16, 20, and 24) are investigated using the method combining the genetic algorithm with density function theory. Benchmark calculation indicates that THSSh functional is reliable to predict the structures of (BeO)(N) cluster. The global minimum structures of (BeO)(N) clusters are rings up to N = 5, double rings at N = 6 and 7 and cages at N ≥ 8. Besides, almost all of the structures of (BeO)(N) cluster are aromatic according to the NICS criterion. Adaptive natural density partitioning analysis reveals that C(6), (BN)(3), and (BeO)(3) rings (C(24) and (BeO)(12) fullerenes) are similar in bonding patterns. The building-up principle of (BeO)(N) is different from that of covalent (BN) and ionic (LiF and MgO) clusters.

  16. The CB1/CB2 receptor agonist WIN-55,212-2 reduces viability of human Kaposi's sarcoma cells in vitro.

    Science.gov (United States)

    Luca, Tonia; Di Benedetto, Giulia; Scuderi, Mariagrazia Rita; Palumbo, Marco; Clementi, Silvia; Bernardini, Renato; Cantarella, Giuseppina

    2009-08-15

    Kaposi's sarcoma is a highly vascularized mesenchymal neoplasm arising with multiple lesions of the skin. Endogenous cannabinoids have been shown to inhibit proliferation of a wide spectrum of tumor cells. We studied the effects of cannabinoids on human Kaposi's sarcoma cell proliferation in vitro. To do so, we first investigated the presence of the cannabinoid receptors CB(1) and CB(2) mRNAs in the human Kaposi's sarcoma cell line KS-IMM by RT-PCR and, subsequently, the effects of the mixed CB(1)/CB(2) agonist WIN-55,212-2 (WIN) on cell proliferation in vitro. WIN showed antimitogenic effects on Kaposi's sarcoma cells. Western blot analysis of Kaposi's sarcoma lysates suggested that WIN treatment induced activation of both caspase-3 and -6, as well as increased phosphorylation of the stress kinase p38 and JNK, along with transient phosphorylation of ERK(1/2). To better characterize the involvement of each single CB receptor in cannabinoid-induced cell death, we incubated Kaposi's sarcoma cells with different selective cannabinoid receptor agonists, respectively ACEA (CB(1)) and JWH-133 (CB(2)). None of the agonists was able to induce KS-IMM cell apoptosis. Moreover, we co-incubated Kaposi's sarcoma cells with WIN-55,212-2 and either the CB(1) receptor antagonist AM251, the CB(2) receptor antagonist AM630, or a combination of both substances. The CB(2) receptor antagonist AM630 was able to significantly increase survival of Kaposi's sarcoma cells treated with WIN. In view of the antiproliferative effects of cannabinoids on KS-IMM cells, one could envision the cannabinoid system as a potential target for pharmacological treatment of Kaposi's sarcoma.

  17. A randomized, multicentre study of directed daylight exposure times of 11/2 vs. 21/2 h in daylight-mediated photodynamic therapy with methyl aminolaevulinate in patients with multiple thin actinic keratoses of the face and scalp

    DEFF Research Database (Denmark)

    Wiegell, S.R.; Fabricius, S.; Philipsen, P.A.

    2011-01-01

    in a randomized multicentre study. Methods: One hundred and twenty patients with a total of 1572 thin AKs of the face and scalp were randomized to either 11/2- or 21/2-h exposure groups. After gentle lesion preparation and application of a sunscreen of sun protection factor 20, MAL was applied to the entire...

  18. Tyrosine 105 and threonine 212 at outermost substrate binding subsites -6 and +4 control substrate specificity, oligosaccharide cleavage patterns, and multiple binding modes of barley alpha-amylase 1

    DEFF Research Database (Denmark)

    Bak-Jensen, K.S.; André, G.; Gottschalk, T.E.;

    2004-01-01

    The role in activity of outer regions in the substrate binding cleft in alpha-amylases is illustrated by mutational analysis of Tyr(105) and Thr(212) localized at subsites - 6 and +4 ( substrate cleavage occurs between subsites -1 and +1) in barley alpha-amylase 1 (AMY1). Tyr(105) is conserved in...

  19. Toxicokinetics of new psychoactive substances: plasma protein binding, metabolic stability, and human phase I metabolism of the synthetic cannabinoid WIN 55,212-2 studied using in vitro tools and LC-HR-MS/MS.

    Science.gov (United States)

    Mardal, Marie; Gracia-Lor, Emma; Leibnitz, Svenja; Castiglioni, Sara; Meyer, Markus R

    2016-10-01

    The new psychoactive substance WIN 55,212-2 ((R)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo-[1,2,3-de]-1,4-benzoxazin-6-yl]-1-napthalenylmethanone) is a potent synthetic cannabinoid receptor agonist. The metabolism of WIN 55,212-2 in man has never been reported. Therefore, the aim of this study was to identify the human in vitro metabolites of WIN 55,212-2 using pooled human liver microsomes and liquid chromatography-high resolution-tandem mass spectrometry (LC-HR-MS/MS) to provide targets for toxicological, doping, and environmental screening procedures. Moreover, a metabolic stability study in pooled human liver microsomes (pHLM) was carried out. In total, 19 metabolites were identified and the following partly overlapping metabolic steps were deduced: degradation of the morpholine ring via hydroxylation, N- and O-dealkylation, and oxidative deamination, hydroxylations on either the naphthalene or morpholine ring or the alkyl spacer with subsequent oxidation, epoxide formation with subsequent hydrolysis, or combinations. In conclusion, WIN 55,212-2 was extensively metabolized in human liver microsomes incubations and the calculated hepatic clearance was comparably high, indicating a fast and nearly complete metabolism in vivo. This is in line with previous findings on other synthetic cannabinoids. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  20. Application of LY-C2-12 D catalyst in C2 hydrogenation isothermal reactor of 330 kt·a-1 ethylene plant%LY-C2-12 D催化剂在330 kt · a-1乙烯装置碳二加氢等温反应器的工业应用

    Institute of Scientific and Technical Information of China (English)

    赵玉龙

    2015-01-01

    中国石油大庆石化公司330 kt·a-1乙烯装置是我国较早成套引进的乙烯装置。该装置碳二加氢一段反应器采用等温反应器,列管之间使用丁烷为冷却介质。第一段反应器采用新型LY-C2-12 D催化剂,运转初期乙烯选择性为80%,运行6个多月,平均选择性达70%,表明该催化剂具有良好的稳定性。%330 kt·a-1 ethylene plant in PetroChina Daqing Petrochemical Co. is one of the earliest im-ported ethylene plants in China. The isothermal reactor is applied as C2 hydrogenation reactor and butane is used for cooling medium among the tubes. The new LY-C2-12D catalyst was applied in the first stage reactor. The selectivity to ethylene reached 80% in initial running period,and the average selectivity was up to 70%,which indicated that the catalyst possessed good stability.

  1. Prolonged exposure to WIN55,212-2 causes downregulation of the CB1 receptor and the development of tolerance to its anticonvulsant effects in the hippocampal neuronal culture model of acquired epilepsy.

    Science.gov (United States)

    Blair, Robert E; Deshpande, Laxmikant S; Sombati, Sompong; Elphick, Maurice R; Martin, Billy R; DeLorenzo, Robert J

    2009-09-01

    Cannabinoids have been shown to cause CB1-receptor-dependent anticonvulsant activity in both in vivo and in vitro models of status epilepticus (SE) and acquired epilepsy (AE). It has been further demonstrated in these models that the endocannabinoid system functions in a tonic manner to suppress seizure discharges through a CB1-receptor-dependent pathway. Although acute cannabinoid treatment has anticonvulsant activity, little is known concerning the effects of prolonged exposure to CB1 agonists and development of tolerance on the epileptic phenotype. This study was carried out to evaluate the effects of prolonged exposure to the CB1 agonist WIN55,212-2 on seizure activity in a hippocampal neuronal culture model of low-Mg(2+) induced spontaneous recurrent epileptiform discharges (SREDs). Following low-Mg(2+) induced SREDs, cultures were returned to maintenance media containing 10, 100 or 1000 nM WIN55,212-2 from 4 to 24 h. Whole-cell current-clamp analysis of WIN55,212-2 treated cultures revealed a concentration-dependent increase in SRED frequency. Immunocytochemical staining revealed that WIN55,212-2 treatment induced a concentration-dependent downregulation of the CB1 receptor in neuronal processes and at both glutamatergic and GABAergic presynaptic terminals. Prolonged exposure to the inactive enantiomer WIN55,212-3 in low-Mg(2+) treated cultures had no effect on the frequency of SREDs or CB1 receptor staining. The results from this study further substantiate a role for a tonic CB1-receptor-dependent endocannabinoid regulation of seizure discharge and suggest that prolonged exposure to cannabinoids results in the development of tolerance to the anticonvulsant effects of cannabinoids and an exacerbation of seizure activity in the epileptic phenotype.

  2. Prolonged exposure to WIN55,212-2 causes down-regulation of the CB1 receptor and the development of tolerance to its anticonvulsant effects in the hippocampal neuronal culture model of acquired epilepsy

    Science.gov (United States)

    Blair, Robert E.; Deshpande, Laxmikant S.; Sombati, Sompong; Elphick, Maurice R.; Martin, Billy R.; DeLorenzo, Robert J.

    2009-01-01

    Summary Cannabinoids have been shown to cause CB1-receptor dependent anticonvulsant activity in both in vivo and in vitro models of status epilepticus (SE) and acquired epilepsy (AE). It has been further demonstrated in these models that the endocannabinoid system functions in a tonic manner to suppress seizure discharges through a CB1-receptor dependent pathway. Although acute cannabinoid treatment has anticonvulsant activity, little is known concerning the effects of prolonged exposure to CB1 agonists and development of tolerance on the epileptic phenotype. This study was carried out to evaluate the effects of prolonged exposure to the CB1 agonist WIN55,212-2 on seizure activity in a hippocampal neuronal culture model of low-Mg2+ induced spontaneous recurrent epileptiform discharges (SREDs). Following low-Mg2+ induced SREDs, cultures were returned to maintenance media containing 10, 100 or 1000 nM WIN55,212-2 from 4 to 24 hours. Whole-cell current-clamp analysis of WIN55,212-2 treated cultures revealed a concentration-dependent increase in SRED frequency. Immunocytochemical staining revealed that WIN55,212-2 treatment induced a concentration-dependent down-regulation of the CB1 receptor in neuronal processes and at both glutamatergic and GABAergic presynaptic terminals. Prolonged exposure to the inactive enantiomer WIN55,212-3 in low-Mg2+ treated cultures had no effect on the frequency of SREDs or CB1 receptor staining. The results from this study further substantiate a role for a tonic CB1 receptor-dependent endocannabinoid regulation of seizure discharge and suggest that prolonged exposure to cannabinoids results in the development of tolerance to the anticonvulsant effects of cannabinoids and an exacerbation of seizure activity in the epileptic phenotype. PMID:19540252

  3. Lower Respiratory Tract Infection Distribution and Drug Resistance of Acinetobacter Baumannii%212例下呼吸道鲍曼不动杆菌感染分布与耐药性研究

    Institute of Scientific and Technical Information of China (English)

    孙淑娟

    2015-01-01

    Objective To shed light on the distribution, resistance characteristics and risk factors of multi-resistant bacteria, and to give clinical guidance for the use of antimicrobial drugs.Methods 2010 April to 2012 April in our hospital 212 cases of patients with lower respiratory tract infection,on 212 strains of Bauman Acinetobacter were investigated,analyzed the distribution,drug resistance and multi drug resistance patients and non multiple drug resistance patients between the susceptible factors.Results 212 cases of lower respiratory tract infection in patients with isolated Bauman Acinetobacter strain 212,among them 80 strains of multiple drug resistance,mainly distributed in the intensive care unit(ICU),folowed by respiratory department of Internal Medicine Department of internal medicine, nerve.2010-2012 years of multidrug resistant Acinetobacter proportion of Bauman were 36.2%,36.7%,39.2%; characteristics of polymyxin B was the most sensitive,folowed by tigecycline;containing beta lactamase inhibitor compound preparation of antibacterial drugs showed obvious resistance.Conclusion Bauman Acinetobacter to polymyxin B was the most sensitive,folowed by tigecycline on carbapenem antibacterial drug drug resistance has gradualy on the rise;the use of different antibiotics,and cardio cerebrovascular disease and ilness change become multi drug resistant bacteria infection major predisposing factors.%目的:探讨212例下呼吸道鲍曼不动杆菌感染分布表现、耐药性特点及多重耐药菌的易感因素。方法2010年4月至2012年4月我院收治212例下呼吸道感染患者,对其分离出的212株鲍曼不动杆菌进行调查,分析其分布、耐药性及多重耐药性患者和非多重耐药性患者两者间的易感因素。结果212例下呼吸道感染患者分离出鲍曼不动杆菌212株,其中多重耐药80株,主要分布在重症监护病房(ICU),其次为呼吸内科、神经内科等。2010-2012年多重耐药的鲍

  4. A novel mutation in GJB1 (c.212T>G) in a Chinese family with X-linked Charcot-Marie-Tooth disease.

    Science.gov (United States)

    Xiao, Fei; Tan, Jia-ze; Zhang, Xu; Wang, Xue-Feng

    2015-03-01

    Gap junction protein beta 1 (GJB1) gene mutations lead to X-linked Charcot-Marie-Tooth (CMTX) disease. We investigated a Chinese family with CMTX and identified a novel GJB1 point mutation. Clinical and electrophysiological features of the pedigree were examined, and sequence alterations of the coding region of GJB1 that encode connexin32 were determined by direct sequencing. Sequence alignment of the mutation site was performed using Clustal W. Mutation effects were analysed using PolyPhen-2, SIFT and Mutation Taster software. The three-dimensional structures of the mutant and wild-type proteins were predicted by modeling with SWISS MODEL online software. The affected family members displayed typical Charcot-Marie-Tooth phenotypes, but phenotypic heterogeneity was observed. Nerve conduction velocities of all affected patients were slow. Sequencing of GJB1 revealed a heterozygous T>G missense mutation at nucleotide 212 in the proband, the proband's mother and the proband's daughter. The affected male sibling of the proband displayed a hemizygous missense mutation with T>G transition at the identical position on the GJB1 gene. This mutation resulted in an amino acid change from isoleucine to serine that was predicted to lead to tertiary structural alterations that would disrupt the function of the GJB1 protein. A novel point mutation in GJB1 was detected, expanding the spectrum of GJB1 mutations known to be associated with CMTX.

  5. Oxidation behaviour of ferritic stainless steel grade Crofer 22 APU at 700 °C in flowing Ar-75%CO2-12%H2O

    Science.gov (United States)

    Shariff, Nurul Atikah; Othman, Norinsan Kamil; Jalar, Azman

    2013-11-01

    The oxidation of Ferritic Stainless Steel (FSS) grade Crofer 22 APU has been investigated. FSS alloys were exposed to isothermal conditions in a horizontal tube furnace at a 700 °C in flowing Ar-75%CO2-12%H2O at a pressure of approximately 1 atm. The results showed that the growth of non protective Fe2O3 and spinel was observed after 50 h exposure in the presence of 12% H2O. The weight was increased significantly with time of exposure. The formation of different oxides is presented on the interface of the specimen such as MnCr2O4, Fe3O4 and Fe2O3 were revealed by X-ray diffraction and supported by EDAX analysis. FSS did not form a protective Cr2O3 layer due to water vapour accelerates the kinetics oxidation. Data of microstructure observation is presented and discussed in this paper in term of water vapour effects.

  6. The enhancement of 21.2%-power conversion efficiency in polymer photovoltaic cells by using mixed Au nanoparticles with a wide absorption spectrum of 400 nm-1000 nm

    Institute of Scientific and Technical Information of China (English)

    郝敬昱; 徐颖; 张玉佩; 陈淑芬; 李兴鳌; 汪联辉; 黄维

    2015-01-01

    Au nanoparticles (NPs) mixed with a majority of bone-like, rod, and cube shapes and a minority of irregu-lar spheres, which can generate a wide absorption spectrum of 400 nm–1000 nm and three localized surface plas-mon resonance peaks, respectively, at 525, 575, and 775 nm, are introduced into the hole extraction layer poly(3,4-ethylenedioxythiophene):poly(4-styrenesulfonate) (PEDOT:PSS) to improve optical-to-electrical conversion performances in polymer photovoltaic cells. With the doping concentration of Au NPs optimized, the cell performance is significantly improved: the short-circuit current density and power conversion efficiency of the poly(3-hexylthiophene): [6,6]-phenyl-C60-butyric acid methyl ester cell are increased by 20.54%and 21.2%, reaching 11.15 mA·cm−2 and 4.23%. The variations of optical, electrical, and morphology with the incorporation of Au NPs in the cells are analyzed in detail, and our results demonstrate that the cell performance improvement can be attributed to a synergistic reaction, including:1) both the local-ized surface plasmon resonance-and scattering-induced absorption enhancement of the active layer, 2) Au doping-induced hole transport/extraction ability enhancement, and 3) large interface roughness-induced efficient exciton dissociation and hole collection.

  7. De novo partial duplication 7(q11.2{r_arrow}q21.2) in a dysmorphic, developmentally retarded boy

    Energy Technology Data Exchange (ETDEWEB)

    Ross, M.; Pinsky, L.; Teebi, A. [McGill Univ., Quebec (Canada)] [and others

    1994-09-01

    Chromosomal abnormalities involving chromosome 7q are rare; we report a case of partial duplication 7q. The propositus was born at 34 weeks by cesarian section, decided because of oligohydramnios, severe intrauterine growth retardation and fetal immobility. At birth, the baby was under the 5th percentile for height, weight and head circumference and had dysmorphic features, including slight asymmetry of the face, bilateral epicanthus, hypoplastic nasal bridge, short globular nose, asymmetrical dysplastic ears, fifth finger clinodactyly, short second and fifth toe. Ultrasound examination showed atrial and ventricular septal defects. At 18 months, the child had a fracture of the femur, secondary to a minor trauma; skeletal X-rays showed generalized osteoporosis and normal healing. The karyotype with GTG-banding showed a de novo partial duplication of the long arm of chromosome 7 (46,XX,dup(7)(q11.23{r_arrow}q21.2)). Fluorescence in situ hybridization with a painting probe specific for chromosome 7 confirmed the intra-chromosomal rearrangement. The patient`s phenotype and his chromosomal abnormality do not match the previously reported cases of partial trisomy 7q. This case confirms the importance of FISH for the delineation of the chromosomal inbalance in structural chromosomal aberrations.

  8. Ruling out unresolved binaries in five transitional disks VLT/NACO: deep 2.12 and 1.75 {\\mu}m narrow-band imaging

    CERN Document Server

    Vicente, Silvia; Hartung, Markus; Bouy, Herve; Huelamo, Nuria; Artigau, Etienne; Augereau, Jean-Charles; van Dishoeck, Ewine; Olofsson, Johan; Oliveira, Isa; Prusti, Timo

    2011-01-01

    Aims. We aim at detecting the presence of companions inside the inner hole/gap region of a sample of five well known transitional disks using spatially-resolved imaging in the near-IR with the VLT/NACO/S13 camera, which probes projected distances from the primary of typically 0.1 to 7 arcsec. The sample includes the stars DoAr 21, HD 135344B (SAO 206462), HR 4796A, T Cha, and TW Hya, spanning ages of less than 1 to 10 Myr, spectral types of A0 to K7, and hole/gap outer radii of 4 to 100 AU. Methods. In order to enhance the contrast and to avoid saturation at the core of the point-spread function (PSF), we use narrow-band filters at 1.75 and 2.12 {\\mu}m. The "locally optimized combination of images" (LOCI) algorithm is applied for an optimal speckle noise removal and PSF subtraction, providing an increase of 0.5-1.5 mag in contrast over the classic method. Results. With the proviso that we could have missed companions owing to unfavorable projections, the VLT/NACO observations rule out the presence of unresolv...

  9. Dynamic expression of miR-132, miR-212, and miR-146 in the brain of different hosts infected with Angiostrongylus cantonensis.

    Science.gov (United States)

    Yu, Liping; Liao, Qi; Chen, Xiaoguang; Xu, Lian; Zeng, Xin; Lv, Zhiyue; Sun, Xi; Zhen, Huanqin; Wu, Zhongdao

    2014-01-01

    Increasing evidence shows that microRNAs (miRNAs) are a family of regulatory molecules involved in many physiological processes, including the inflammation in central nervous system (CNS) and neurological disorders. Angiostrongylus cantonensis (A. cantonensis) is the major cause of human infectious eosinophilic meningitis and can induce CNS injury. In the present study, we investigated the expression of miRNAs involved in neuronal functions such as miR-132-3p/212-3p, and miR-146a-5p, inflammation-related miRNA, in the modulation of inflammation of CNS of mice and rats induced by A. cantonensis. The functions of differentially expressed miRNAs were analyzed through bioinformatics methods, and the expression of chosen target genes were investigated by quantitative reverse transcription polymerase chain reaction. The results showed that miR-146a-5p upregulated in the brain of rats after 21 days; A. cantonensis infection and the expression of miR-132-3p and miR-146a-5p upregulated in the brain of mice model infected by A. cantonensis. The expression of the target genes of mmu-miR-146a-5p such as Irak1 and Traf6 downregulated in 14 days and 21 days after A. cantonensis infection. Our results supply more information about the involvement of the miRNAs in the regulation of inflammation of CNS induced by A. cantonensis.

  10. Comprehensive study on the structure of the BSA from extended-to aged form in wide (2-12) pH range.

    Science.gov (United States)

    Varga, N; Hornok, V; Sebők, D; Dékány, I

    2016-07-01

    In this work we studied the structure of the bovine serum albumin (BSA) and the protein-ligand interactions since researchers prefer to use them as carriers in drug delivery systems. Systematic study (between pH 2-12, in double distilled water and physiological salt solution) was carried out to determine the changes in the secondary and the tertiary structures of the BSA, the apparent molecular weight (Mw), the size (dLS) and the electrokinetic potential (ζ). At pH 7, the BSA has higher stability in the absence (ζ=-69mV, dLS=2.2nm, A2=1.4×10(-3)mlmol/g(2)) than in the presence of salt solution (ζ=-2.4mV, dLS=5.3nm, A2=-3.2×10(-4)mlmol/g(2)). The Mw strongly depends on the pH and the ionic strength (at pH 3 in the absence of salt, the Mw is 54.6kDa while in the presence of salt is 114kDa) which determines the geometry of the protein. The protein-ligand interactions were characterized by fluorescence (FL) and isothermal microcalorimetry (ITC) methods; these independent techniques provided similar thermodynamic parameters such as the binding constant (K) and the Gibbs free energy (ΔG).

  11. Why Is The Neutrino Oscillation Formula Expanded In $\\Delta m_{21}^{2}/\\Delta m_{31}^{2}$ Still Accurate Near The Solar Resonance In Matter?

    CERN Document Server

    Xu, Xun-Jie

    2015-01-01

    The traditional approximate formula for neutrino oscillation in matter which is obtained from the expansion in terms of the ratio of mass square differences $\\alpha=\\Delta m_{21}^{2}/\\Delta m_{31}^{2}\\approx0.03$, first proposed by Cervera, et al and Freund, turns out to be an accurate formula for accelerator neutrino experiments. It is accurate not only at energies well above the solar resonance as required by the validity of the $\\alpha-$expansion but also at energies near the solar resonance which is just the case of the T2K experiment. Due to the non-perturbative effect of the solar resonance, the $\\alpha-$expansion in this case is invalid but this formula was recently used in T2K. This paper analytically justifies the use of this formula in T2K by careful dealing with the singularity originated from the branch cut in the roots of the equation for the eigenvalues. Besides, we also propose some simple but more accurate formulae which provide possible alternatives to the traditional formula.

  12. Localisation of the gene for Saethre-Chotzen syndrome by FISH using four cases with apparently balanced translocations at 7p21.2

    Energy Technology Data Exchange (ETDEWEB)

    Rose, C.S.P.; King, A.A.J.; Winter, R.M. [Institute of Child Health, London (United Kingdom)] [and others

    1994-09-01

    Saethre-Chotzen is a common autosomal dominant form of craniosynostosis, which results in the premature fusion of cranial sutures. The Saethre-Chotzen gene locus has been mapped to 7p by linkage with genetic markers. We have analyzed four patients with Saethre-Chotzen syndrome associated with apparently balanced translocations involving band 7p21.2 and different reciprocal chromosomes. We have used YACs corresponding to the Genethon series of markers as probes for FISH, and in all four patients we show that the breakpoints in 7p are situated between D7S488 and D7S493, a maximum distance of 6 centimorgans. A previous patient has been described with a translocation t(6;7) and hemizygosity for D7S135. D7S135 is contained within the same YAC as D7S488, indicating that the breakpoint in the previous patient lies close to D7S488 and in the same region as the cases described here.

  13. High Precision 2.0 μm Photoacoustic Spectrometer for Determination of the ^{13}CO_{2}/^{12}CO_{2} Isotope Ratio

    Science.gov (United States)

    Reed, Zachary; Hodges, Joseph T.

    2017-06-01

    We have developed a portable photoacoustic spectrometer for high precision measurements of the ^{13}CO_{2}/^{12}CO_{2} isotope ratio and the absolute molar concentration of each isotope. The spectrometer extends on our previous work at 1.57 μm [1], and now employs two separate intensity modulated distributed feedback lasers and a fiber amplifier, operating in the 2.0 μm wavelength region. Each DFB is selected to probe individual spectrally isolated ro-vibrational transitions for ^{12}CO_{2} and ^{13}CO_{2}. The spectrometer is actively temperature controlled, mitigating variations in the two spectral line intensities and the temperature dependent system response. For measurements of ambient concentrations of carbon dioxide at nominally natural abundance in dry air, we demonstrate a measurement precision of 140 ppb for ^{12}CO_{2} with a 1 s averaging time and 10 ppb for ^{13}CO_{2} with a 60 s averaging time. Precision in δ13C of better than 0.1 permil is demonstrated. The analyzer response is calibrated in terms of certified gas mixtures and compared to characterization by cavity ringdown spectroscopy. We also investigate how water vapor affects the photoacoustic signals by promoting collisional relaxation for each isotope. [1] Z.D. Reed, B. Sperling, et al. App. Phys. B. 117, 645-657, 2014

  14. 妊娠期高血压疾病终止妊娠时机及分娩方式212例临床探讨%Hypertensive Disorder Complicating Pregnancy Termination of the Clinical Study of 212 Cases of Pregnancy and Delivery Time

    Institute of Scientific and Technical Information of China (English)

    王骧; 陈艺菲

    2013-01-01

    Objective: To investigate the best time in patients with pregnancy induced hypertension and termination of pregnancy,as wel as the correct mode of delivery. Methods: from 2011June to 2013 June in patients with hypertension of pregnancytreated in our hospital, a total of 212 cases, the clinicaltreatment, these patients track record, combined with asphyxia,neonatal birth after uterine bleeding were analyzed. Results: 212 cases of patients, 31 cases of delivery mode for vaginal delivery, 182 cases of cesarean section, Caesarean birth 85.85%; vaginal delivery occurred postpartum hemorrhage in 13 cases, accounting for 6.12% of the total, cesarean deliveryin patients with postpartum hemorrhage in 16 cases, accounting for 7.54% of the total. 30.24% appear in vaginal delivery,neonatal asphyxia, cesarean section in 12.31%newborns with asphyxia. Vaginal delivery in neonatal asphyxia is higher than that of cesarean section (P> 0.05). Conclusion: for the patients with hypertensive disorder complicating pregnancy, when is the best time for termination of pregnancy is inconclusive, according to the research results and research data shows, in patients with pregnancy induced hypertension of pregnancy termination timing according to clinical manifestations, gestational weeks,the progression of the disease, drug treatment and fetal development comprehensively evaluation and discussion.%目的:探讨妊娠期高血压患者终止妊娠的最佳时机,以及正确的分娩方式。方法选取2011年6月~2013年6月在我院治疗的妊娠期高血压患者,共212例,对这些患者的临床治疗情况、跟踪记录情况,结合分娩后子宫出血情况、新生儿的窒息情况进行全面分析。结果212例患者中,31例分娩方式为阴道分娩,182例为剖宫产,剖宫产占85.85%;阴道分娩的发生产后出血13例,占总数的6.12%,剖宫产分娩的患者发生产后出血16例,占总数的7.54%。阴道分娩中30.24%出现

  15. Systemic radioimmunotherapy using a monoclonal antibody, anti-Tac directed toward the alpha subunit of the IL-2 receptor armed with the {alpha}-emitting radionuclides {sup 212}Bi or {sup 211}At

    Energy Technology Data Exchange (ETDEWEB)

    Wesley, Jon N.; McGee, Edwin C.; Garmestani, Kayhan; Brechbiel, Martin W.; Yordanov, Alexander T.; Wu Chuanchu; Gansow, Otto A.; Eckelman, William C.; Bacher, John D.; Flynn, Michael; Goldman, Carolyn K.; MacLin, Melvin; Schwartz, Uwe P.; Jackson-White, Terri; Phillip, Celeste M.; Decker, Jean; Waldmann, Thomas A. E-mail: tawald@helix.nih.gov

    2004-04-01

    To exploit the fact that IL-2 receptors are expressed by T-cells responding to foreign antigens but not by resting T-cells, humanized anti-Tac (HAT) armed with alpha-emitting radionuclides {sup 212}Bi and {sup 211}At was evaluated in a cynomolgus cardiac allograft model. Control graft survival was 8.2{+-} 0.5 days compared with 14.0{+-}1.3 days (p<0.01) survival for monkeys treated with {sup 212}Bi labeled HAT and 26.7{+-}2.4 days survival (p<0.001 versus controls) with {sup 211}At labeled HAT. Thus, {sup 211}At labeled HAT may have application in organ transplantation and in treatment of IL-2 receptor expressing T-cell leukemia.

  16. Calibrated cryo-cell UV-LA-ICPMS elemental concentrations from the NGRIP ice core reveal abrupt, sub-annual variability in dust across the GI-21.2 interstadial period

    Science.gov (United States)

    Della Lunga, Damiano; Müller, Wolfgang; Olander Rasmussen, Sune; Svensson, Anders; Vallelonga, Paul

    2017-05-01

    Several abrupt shifts from periods of extreme cold (Greenland stadials, GS) to relatively warmer conditions (Greenland interstadials, GI) called Dansgaard-Oeschger events are recorded in the Greenland ice cores. Using cryo-cell UV-laser-ablation inductively coupled-plasma mass spectrometry (UV-LA-ICPMS), we analysed a 2.85 m NGRIP ice core section (2691.50-2688.65 m depth, age interval 84.86-85.09 ka b2k, thus covering ˜ 230 years) across the transitions of GI-21.2, a short-lived interstadial prior to interstadial GI-21.1. GI-21.2 is a ˜ 100-year long period with δ18O values 3-4 ‰ higher than the following ˜ 200 years of stadial conditions (GS-21.2), which precede the major GI-21.1 warming. We report concentrations of major elements indicative of dust and/or sea salt (Na, Fe, Al, Ca, Mg) at a spatial resolution of ˜ 200 µm, while maintaining detection limits in the low-ppb range, thereby achieving sub-annual time resolution even in deep NGRIP ice. We present an improved external calibration and quantification procedure using a set of five ice standards made from aqueous (international) standard solutions. Our results show that element concentrations decrease drastically (more than 10-fold) at the warming onset of GI-21.2 at the scale of a single year, followed by relatively low concentrations characterizing the interstadial part before gradually reaching again typical stadial values.

  17. Remaining Sites Verification Package for the 100-B-21:2 Subsite (100-B/C Discovery Pipeline DS-100BC-002), Waste Site Reclassification Form 2008-003

    Energy Technology Data Exchange (ETDEWEB)

    J. M. Capron

    2008-06-16

    The 100-B-21:2 waste site consists of the immediate area of the DS-100BC-02 pipeline. In accordance with this evaluation, the confirmatory and verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River.

  18. Influence of intracerebroventricular or intraperitoneal administration of cannabinoid receptor agonist (WIN 55,212-2) and inverse agonist (AM 251) on the regulation of food intake and hypothalamic serotonin levels.

    Science.gov (United States)

    Merroun, Ikram; Errami, Mohammed; Hoddah, Hanaa; Urbano, Gloria; Porres, Jesús M; Aranda, Pilar; Llopis, Juan; López-Jurado, María

    2009-05-01

    The effect of intracerebroventricular or intraperitoneal administration of cannabinoid receptor agonist WIN 55,212-2 or inverse agonist AM 251 on food intake and extracellular levels of serotonin and acetic acid 5-hydroxy-indol from presatiated rats was studied. Compared to the vehicle-injected control, the intracerebroventricular administration of WIN 55,212-2 was associated with a significant increase in food intake, whereas the administration of AM 251 caused a significant reduction in this respect. These results were accompanied by considerable reductions or increases in serotonin and acetic acid 5-hydroxy-indol levels compared to the vehicle-injected control and the baseline values for the different experimental groups studied. Intraperitoneal administration of WIN 55,212-2 at doses of 1 and 2 mg/kg promoted hyperphagia up to 6 h after injection, whereas administration of a higher dose (5 mg/kg) significantly inhibited food intake and motor behaviour in partially satiated rats. Administration of any of the AM 251 doses studied (0.5, 1, 2, 5 mg/kg) led to a significant decrease in the amount of food ingested from 2 h after the injection, compared to the vehicle-injected control group, with the most striking effect being observed when the 5 mg/kg dose was injected.

  19. Cannabinoid type 1 receptor ligands WIN 55,212-2 and AM 251 alter anxiety-like behaviors of marmoset monkeys in an open-field test.

    Science.gov (United States)

    Cagni, Priscila; Barros, Marilia

    2013-03-01

    Cannabinoid type 1 receptors (CB1r) are an important modulatory site for emotional behavior. However, little is known on the effects of CB1r ligands on emotionality aspects of primates, even with their highly similar behavioral response and receptor density/distribution as humans. Thus, we analyzed the effects of the CB1r agonist WIN 55,212-2 (WIN; 1mg/kg) and the antagonist AM 251 (AM; 2mg/kg), systemically administered prior to a single brief (15 min) exposure to a novel open-field (OF) environment, on the behavior of individually tested adult black tufted-ear marmosets. Both WIN- and AM-treated subjects, compared to vehicle controls, had significantly lower rates of long (contact) calls and exploration, while higher levels of vigilance-related behaviors (scan/glance); these are indicators of anxiolysis in this setup. Changes in locomotion were not detected. However, in the vehicle and AM-groups, sojourn in the peripheral zone of the OF was significantly higher than in its central region. WIN-treated marmosets spent an equivalent amount of time in both zones. Therefore, activation or blockade CB1r function prior to a short and individual exposure to an unfamiliar environment exerted a significant and complex influence on different behavioral indicators of anxiety in these monkeys (i.e., a partially overlapping anxiolytic-like profile). AM 251, however, has no anxiolytic effect when the time spent in the center of the OF is considered. This is a major difference when compared to the WIN-treated group. Data were compared to the response profile reported in other pre-clinical (rodent) and clinical studies.

  20. Enhanced self-administration of the CB1 receptor agonist WIN55,212-2 in olfactory bulbectomized rats: evaluation of possible serotonergic and dopaminergic underlying mechanisms

    Directory of Open Access Journals (Sweden)

    Petra eAmchova

    2014-03-01

    Full Text Available Depression has been associated with drug consumption, including heavy or problematic cannabis use. According to an animal model of depression and substance use disorder comorbidity, we combined the olfactory bulbectomy model of depression with intravenous drug self-administration procedure to verify whether depressive-like rats displayed higher voluntary intake of the CB1 receptor agonist WIN55,212-2 (WIN, 12.5 µg/kg/infusion. To this aim, olfactory-bulbectomized (OBX and sham-operated (SHAM Lister Hooded rats were allowed to self-administer WIN by lever-pressing under a continuous (FR-1 schedule of reinforcement in 2h daily sessions. Data showed that both OBX and SHAM rats developed stable WIN intake; yet, responses in OBX were constantly higher than in SHAM rats soon after the first week of training. In addition, OBX rats took significantly longer to extinguish the drug-seeking behaviour after vehicle substitution. Acute pre-treatment with serotonin 5HT1B receptor agonist, CGS-12066B (2.5-10 mg/kg, did not significantly modify WIN intake in OBX and SHAM Lister Hooded rats. Furthermore, acute pre-treatment with CGS-12066B (10 and 15 mg/kg did not alter responses in parallel groups of OBX and SHAM Sprague Dawley rats self-administering methamphetamine under higher (FR-2 reinforcement schedule with nose-poking as operandum. Finally, dopamine levels in the nucleus accumbens of OBX rats did not increase in response to a WIN challenge, as in SHAM rats, indicating a dopaminergic dysfunction in bulbectomized rats. Altogether, our findings suggest that a depressive state may alter cannabinoid CB1 receptor agonist-induced brain reward function and that a dopaminergic rather than a 5-HT1B mechanism is likely to underlie enhanced WIN self-administration in OBX rats.

  1. Paracentric inversion of chromosome 7 (46,XX,inv(7)(q21.2q22)) in a newborn with hypoplastic left heart syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Kuforjii, T.A.; Pillers, D.M.; Silberbach, M. [Oregon Health Sciences Univ., Portland, OR (United States)] [and others

    1994-09-01

    Hypoplastic left heart syndrome (HLHS) is a severe congenital heart disease that is uniformly fatal without surgical intervention. Fetal echocardiography allows prenatal diagnosis, but this condition may not become apparent until after the mid-second trimester. We report a term baby with severe HLHS who had an 18 week fetal ultrasound that reportedly demonstrated a normal heart. There was no family history of congenital heart disease. She was phenotypically female with no dysmorphic features. Physical examination was otherwise normal. She expired at 48 hours of age. The autopsy was noncontributory. The karyotype was 46,XX, but there was an apparently balanced paracentric inversion of the long arm of chromosome 7 (46,XX,inv(7)(q21.2q22)). The mother`s chromosome study was normal without any inversion, and the father was not available for study. Hypoplastic left heart syndrome has been associated with extracardiac anomalies and chromosomal abnormalities including 45,XO,11q-, and trisomy 18. It has also been reported in 5 members spanning 3 generations of a family with a spectrum of left heart defects suggesting an autosomal dominant pattern with high penetrance. First-degree relatives of infants with HLHS have a thirteen percent incidence of related cardiovascular malformations, a frequency higher than predicted by a multifactorial model of inheritance, suggesting that at least a portion of HLHS have a genetic basis. Karyotype analysis, including high resolution banding, may help define the etiology of this condition. Chromosome 7 has not been implicated in HLHS. This case emphasizes the need for genetic analysis, including a pedigree, of affected families. It also underscores the importance of screening by karyotype analysis to determine whether defects of the long arm of chromosome 7 are important in the pathogenesis of hypoplastic left heart syndrome.

  2. Cannabinoid WIN-55,212-2 mesylate inhibits interleukin-1β induced matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression in human chondrocytes.

    Science.gov (United States)

    Dunn, S L; Wilkinson, J M; Crawford, A; Le Maitre, C L; Bunning, R A D

    2014-01-01

    Interleukin-1β (IL-1β) is involved in the up-regulation of matrix metalloproteinases (MMPs) leading to cartilage degradation. Cannabinoids are anti-inflammatory and reduce joint damage in animal models of arthritis. This study aimed to determine a mechanism whereby the synthetic cannabinoid WIN-55,212-2 mesylate (WIN-55) may inhibit cartilage degradation. Effects of WIN-55 were studied on IL-1β stimulated production of MMP-3 and -13 and their inhibitors TIMP-1 and -2 in human chondrocytes. Chondrocytes were obtained from articular cartilage of patients undergoing total knee replacement. Chondrocytes were grown in monolayer and 3D alginate bead cultures. Real-time polymerase chain reaction (PCR) was used to determine the gene expression of MMP-3, -13, TIMP-1 and -2 and Enzyme Linked Immunosorbent Assay (ELISA) to measure the amount of MMP-3 and MMP-13 protein released into media. Immunocytochemistry was used to investigate the expression of cannabinoid receptors in chondrocyte cultures. Treatment with WIN-55 alone or in combination with IL-1β, decreased or abolished MMP-3, -13, TIMP-1 and -2 gene expression in human chondrocyte monolayer and alginate bead cultures in both a concentration and time dependent manner. WIN-55 treatment alone, and in combination with IL-1β, reduced MMP-3 and -13 protein production by chondrocytes cultured in alginate beads. Immunocytochemistry demonstrated the expression of cannabinoid receptors in chondrocyte cultures. Cannabinoid WIN-55 can reduce both basal and IL-1β stimulated gene and protein expression of MMP-3 and -13. However WIN-55 also decreased basal levels of TIMP-1 and -2 mRNA. These actions of WIN-55 suggest a mechanism by which cannabinoids may act to prevent cartilage breakdown in arthritis. Copyright © 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

  3. THE CANNABINOID WIN 55,212-2 DECREASES SPECIFICITY PROTEIN (Sp) TRANSCRIPTION FACTORS AND THE ONCOGENIC CAP PROTEIN eIF4E IN COLON CANCER CELLS

    Science.gov (United States)

    Sreevalsan, Sandeep; Safe, Stephen

    2013-01-01

    2,3-Dihydro-5-methyl-3-([morpholinyl]methyl)pyrollo(1,2,3-de)-1,4-benzoxazinyl]-[1-naphthaleny]methanone [WIN 55,212-2 (WIN)] is a synthetic cannabinoid that inhibits RKO, HT-29 and SW480 cell growth, induced apoptosis, and downregulated expression of survivin, cyclin D1, epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF) and its receptor (VEGFR1). WIN also decreased expression of specificity protein (Sp) transcription factors Sp1, Sp3 and Sp4, and this is consistent with the observed downregulation of the aforementioned Sp-regulated genes. In addition, we also observed by RNA interference (RNAi) that the oncogenic cap protein eIF4E was an Sp-regulated gene also downregulated by WIN in colon cancer cells. WIN-mediated repression of Sp proteins was not affected by CB receptor antagonists or by knockdown of the receptor but was attenuated by the phosphatase inhibitor sodium orthovanadate or by knockdown of protein phosphatase 2A (PP2A). WIN-mediated repression of Sp1, Sp3 and Sp4 was due to PP2A-dependent downregulation of microRNA-27a (miR-27a) and induction of miR-27a-regulated ZBTB10 which has previously been characterized as an “Sp repressor”. The results demonstrate that the anticancer activity of WIN is due, in part, to PP2A-dependent disruption of miR-27a:ZBTB10 and ZBTB10-mediated repression of Sp transcription factors and Sp-regulated genes including eIF4E. PMID:24030632

  4. Involvement of TRPV1 channels in the activity of the cannabinoid WIN 55,212-2 in an acute rat model of temporal lobe epilepsy.

    Science.gov (United States)

    Carletti, Fabio; Gambino, Giuditta; Rizzo, Valerio; Ferraro, Giuseppe; Sardo, Pierangelo

    2016-05-01

    The exogenous cannabinoid agonist WIN 55,212-2, (R)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinylmethyl) pyrrolo[1,2,3-de]-1,4-benzoxazin-6-Yl]-1-naphthalenylmethanone (WIN), has revealed to play a role on modulating the hyperexcitability phenomena in the hippocampus. Cannabinoid-mediated mechanisms of neuroprotection have recently been found to imply the modulation of transient receptor potential vanilloid 1 (TRPV1), a cationic channel subfamily that regulate synaptic excitation. In our study, we assessed the influence of pharmacological manipulation of TRPV1 function, alone and on WIN antiepileptic activity, in the Maximal Dentate Activation (MDA) acute model of temporal lobe epilepsy. Our results showed that the TRPV1 agonist, capsaicin, increased epileptic outcomes; whilst antagonizing TRPV1 with capsazepine exerts a protective role on paroxysmal discharge. When capsaicin is co-administered with WIN effective dose of 10mg/kg is able to reduce its antiepileptic strength, especially on the triggering of MDA response. Accordingly, capsazepine at the protective dose of 2mg/kg managed to potentiate WIN antiepileptic effects, when co-treated. Moreover, WIN subeffective dose of 5mg/kg was turned into effective when capsazepine comes into play. This evidence suggests that systemic administration of TRPV1-active drugs influences electrically induced epilepsy, with a noticeable protective activity for capsazepine. Furthermore, results from the pharmacological interaction with WIN support an interplay between cannabinoid and TRPV1 signaling that could represent a promising approach for a future pharmacological strategy to challenge hyperexcitability-based diseases.

  5. [Health promotion effectiveness: testing the German statutory health insurance agencies evaluation system in health promotion, and preliminary findings from 212 health training courses].

    Science.gov (United States)

    Kliche, T; Schreiner-Kürten, K; Wanek, V; Koch, U

    2011-04-01

    The purpose of this study was to develop a measurement system for the evaluation of health promotion training courses offered by German statutory health insurance companies. In a field test, N=1 671 participants from 212 youth and adult courses for the promotion of either physical activity, coping with stress or nutritional improvement were included. 80% were female. Participants were questioned in a pre-post-design with a three month follow-up. The questionnaires covered health behaviour and health status. Participants' compliance and psychometric quality of the measurement instruments were good. On average, the health insurance companies assigned participants to different interventions adequately according to the participant's individual health problems. The health promotion courses triggered improvements of high effect sizes for health behaviour patterns, of moderate effect sizes for physical complaints, subjective health ratings, and health-related quality of life. Effects decreased after the end of the intervention but remained significantly above the initial values. BMI values continued their improvement after the end of the training courses. Thus, health promotion training courses generated stable health improvements of practically relevant effect sizes. The interventions provided good support and health improvements for all subgroups of participants, regardless of age, gender and educational background. Thus, the health promotion curricula of the health insurance companies offer a ubiquitous and easily accessible but effective intervention for health promotion in Germany, although men are clearly underrepresented among the participants. The trainings may be recommended and used by other health-care suppliers. The evaluation toolkit provides practical and valid instruments for a routine evaluation of health promotion trainings. It should be applied within random sampling designs. © Georg Thieme Verlag KG Stuttgart · New York.

  6. Health and exercise-related medical issues among 1,212 ultramarathon runners: baseline findings from the Ultrarunners Longitudinal TRAcking (ULTRA Study.

    Directory of Open Access Journals (Sweden)

    Martin D Hoffman

    Full Text Available Regular exercise is associated with substantial health benefits; however, little is known about the health impact of extreme levels of exercise. This study examined the prevalence of chronic diseases, health-care utilization, and risk factors for exercise-related injuries among ultramarathon runners. Retrospective, self-reported enrollment data from an ongoing longitudinal observational study of 1,212 active ultramarathon runners were analyzed. The most prevalent chronic medical conditions were allergies/hay fever (25.1% and exercise-induced asthma (13.0%, but there was a low prevalence of serious medical issues including cancers (4.5%, coronary artery disease (0.7%, seizure disorders (0.7%, diabetes (0.7%, and human immunodeficiency virus (HIV infection (0.2%. In the year preceding enrollment, most (64.6% reported an exercise-related injury that resulted in lost training days (median of 14 days, but little nonattendance of work or school due to illness, injury, or exercise-related medical conditions (medians of 0 days for each. The knee was the most common area of exercise-related injury. Prior year incidence of stress fractures was 5.5% with most (44.5% involving the foot. Ultramarathon runners who sustained exercise-related injuries were younger (p<0.001 and less experienced (p<0.01 than those without injury. Stress fractures were more common (p<0.01 among women than men. We conclude that, compared with the general population, ultramarathon runners appear healthier and report fewer missed work or school days due to illness or injury. Ultramarathon runners have a higher prevalence of asthma and allergies than the general population, and the prevalence of serious medical issues was nontrivial and should be recognized by those providing medical care to these individuals. Ultramarathon runners, compared with shorter distance runners, have a similar annual incidence of exercise-related injuries but higher proportion of stress fractures involving

  7. Involvement of dorsal hippocampal and medial septal nicotinic receptors in cross state-dependent memory between WIN55, 212-2 and nicotine or ethanol in mice.

    Science.gov (United States)

    Alijanpour, S; Rezayof, A

    2013-08-15

    The present study examined whether nicotinic acetylcholine receptors (nAChRs) of the CA1 regions of the dorsal hippocampus and medial septum (MS) are involved in cross state-dependent memory retrieval between WIN55, 212-2 (WIN, a non-selective CB1/CB2 receptor agonist) and nicotine or ethanol. Memory retrieval was measured in one-trial step-down type passive avoidance apparatus in male adult mice. Pre-training intraperitoneal administration of WIN (0.1-1mg/kg) dose-dependently impaired memory retrieval when it was tested 24h later. Pre-test systemic administration of nicotine (0.6 and 0.7mg/kg, s.c.) or ethanol (0.5g/kg, i.p.) improved WIN-induced memory impairment, suggesting a cross state-dependent memory retrieval between the drugs. Pre-test intra-CA1 microinjection of nicotine (1 and 2μg/mouse) before systemic administration of an ineffective dose of nicotine (0.5mg/kg, s.c.) or ethanol (0.25g/kg) significantly reversed WIN-induced memory impairment. Pre-test intra-CA1 microinjection of mecamylamine (1 and 3μg/mouse) inhibited cross state-dependent memory between WIN and nicotine or ethanol. Moreover, pre-test intra-MS microinjection of nicotine (1 and 2μg/mouse) in combination with systemic administration of a lower dose of nicotine (0.5mg/kg), but not ethanol (0.25g/kg), improved memory impairment induced by pre-training administration of WIN. On the other hand, in the animals that received pre-training WIN and pre-test systemic administration of nicotine (0.7mg/kg), but not ethanol (0.5g/kg), pre-test intra-MS microinjection of mecamylamine (1-5μg/mouse) inhibited WIN-nicotine state-dependent memory retrieval. It should be noted that pre-test intra-CA1 or intra-MS microinjection of nicotine or mecamylamine by itself had no effect on memory retrieval and also could not reverse memory impairment induced by pre-training administration of WIN. It can be concluded that WIN and nicotine or WIN and ethanol can induce state-dependent memory retrieval. In

  8. Anti-inflammatory effect of cannabinoid agonist WIN55, 212 on mouse experimental colitis is related to inhibition of p38MAPK.

    Science.gov (United States)

    Feng, Ya-Jing; Li, Yong-Yu; Lin, Xu-Hong; Li, Kun; Cao, Ming-Hua

    2016-11-21

    To investigate the anti-inflammatory effect and the possible mechanisms of an agonist of cannabinoid (CB) receptors, WIN55-212-2 (WIN55), in mice with experimental colitis, so as to supply experimental evidence for its clinical use in future. We established the colitis model in C57BL/6 mice by replacing the animals' water supply with 4% dextran sulfate sodium (DSS) for 7 consecutive days. A colitis scoring system was used to evaluate the severity of colon local lesion. The plasma levels of proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), and the myeloperoxidase (MPO) activity in colon tissue were measured. The expressions of cannabinoid receptors, claudin-1 protein, p38 mitogen-activated protein kinase (p38MAPK) and its phosphorylated form (p-p38) in colon tissue were determined by immunohistochemistry and Western blot. In addition, the effect of SB203580 (SB), an inhibitor of p38, was investigated in parallel experiments, and the data were compared with those from intervention groups of WIN55 and SB alone or used together. The results demonstrated that WIN55 or SB treatment alone or together improved the pathological changes in mice with DSS colitis, decreased the plasma levels of TNF-α, and IL-6, and MPO activity in colon. The enhanced expression of claudin-1 and the inhibited expression of p-p38 in colon tissues were found in the WIN55-treated group. Besides, the expression of CB1 and CB2 receptors was enhanced in the colon after the induction of DSS colitis, but reduced when p38MAPK was inhibited. These results confirmed the anti-inflammatory effect and protective role of WIN55 on the mice with experimental colitis, and revealed that this agent exercises its action at least partially by inhibiting p38MAPK. Furthermore, the results showed that SB203580, affected the expression of CB1 and CB2 receptors in the mouse colon, suggesting a close linkage and cross-talk between the p38MAPK signaling pathway and the

  9. Anti-inflammatory effect of cannabinoid agonist WIN55, 212 on mouse experimental colitis is related to inhibition of p38MAPK

    Science.gov (United States)

    Feng, Ya-Jing; Li, Yong-Yu; Lin, Xu-Hong; Li, Kun; Cao, Ming-Hua

    2016-01-01

    AIM To investigate the anti-inflammatory effect and the possible mechanisms of an agonist of cannabinoid (CB) receptors, WIN55-212-2 (WIN55), in mice with experimental colitis, so as to supply experimental evidence for its clinical use in future. METHODS We established the colitis model in C57BL/6 mice by replacing the animals’ water supply with 4% dextran sulfate sodium (DSS) for 7 consecutive days. A colitis scoring system was used to evaluate the severity of colon local lesion. The plasma levels of proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), and the myeloperoxidase (MPO) activity in colon tissue were measured. The expressions of cannabinoid receptors, claudin-1 protein, p38 mitogen-activated protein kinase (p38MAPK) and its phosphorylated form (p-p38) in colon tissue were determined by immunohistochemistry and Western blot. In addition, the effect of SB203580 (SB), an inhibitor of p38, was investigated in parallel experiments, and the data were compared with those from intervention groups of WIN55 and SB alone or used together. RESULTS The results demonstrated that WIN55 or SB treatment alone or together improved the pathological changes in mice with DSS colitis, decreased the plasma levels of TNF-α, and IL-6, and MPO activity in colon. The enhanced expression of claudin-1 and the inhibited expression of p-p38 in colon tissues were found in the WIN55-treated group. Besides, the expression of CB1 and CB2 receptors was enhanced in the colon after the induction of DSS colitis, but reduced when p38MAPK was inhibited. CONCLUSION These results confirmed the anti-inflammatory effect and protective role of WIN55 on the mice with experimental colitis, and revealed that this agent exercises its action at least partially by inhibiting p38MAPK. Furthermore, the results showed that SB203580, affected the expression of CB1 and CB2 receptors in the mouse colon, suggesting a close linkage and cross-talk between the p38

  10. Cannabinoid WIN-55,212-2 mesylate inhibits ADAMTS-4 activity in human osteoarthritic articular chondrocytes by inhibiting expression of syndecan-1

    Science.gov (United States)

    KONG, YING; WANG, WANCHUN; ZHANG, CHANGJIE; WU, YI; LIU, YANG; ZHOU, XIAORONG

    2016-01-01

    A central feature of osteoarthritis (OA) is the loss of articular cartilage, which is primarily attributed to cartilage breakdown. A group of metalloproteinases termed the A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family are reported to be important in cartilage breakdown. Recent studies have suggested that ADAMTS-4 is a major contributor to the pathogenesis of OA and that syndecan-1 is closely associated with activation of ADAMTS-4 in human chondrocytes. Accumulating evidence also suggests that cannabinoids have chondroprotective effects. The current study explored the effects of synthetic cannabinoid WIN-55,212-2 mesylate (WIN-55) on the expression of syndecan-1 and ADAMTS-4, as well as ADAMTS-4 activity, in unstimulated and interleukin (IL)-1β-stimulated OA chondrocytes. Primary human OA articular chondrocytes were treated with WIN-55 in the presence or absence of IL-1β and cannabinoid receptor antagonists. The results of the present study demonstrated that WIN-55 inhibited ADAMTS-4 activity in unstimulated and IL-1β-stimulated primary human OA articular chondrocytes in a concentration-dependent manner. Cannabinoid receptor type 1 (CB1) and 2 (CB2) were constitutively expressed in human OA articular chondrocytes. Furthermore, selective CB2 antagonist, JTE907, but not selective CB1 antagonist, MJ15, abolished the inhibitory effect of WIN-55 on ADAMTS-4 activity. WIN55 inhibited the expression of syndecan-1 but not ADAMTS-4, and overexpression of syndecan-1 reversed the inhibitory effect of WIN-55 on the ADAMTS-4 activity in unstimulated and IL-1β-stimulated human OA articular chondrocytes. Despite having no significant effect on syndecan-1 gene promoter activity, WIN-55 markedly decreased the stability of syndecan-1 mRNA via CB2. In conclusion, to the best of our knowledge, the present study provides the first in vitro evidence supporting that the synthetic cannabinoid WIN-55 inhibits ADAMTS-4 activity in unstimulated and IL-1

  11. 大麻素WIN55,212-2对裸小鼠肝癌移植瘤及PPARγ表达的影响%Effect of cannabinoid WIN55,212-2 on HepG2 tumor xenograft and PPAR-γexpression in nude mice

    Institute of Scientific and Technical Information of China (English)

    邓远斐; 许达才; 肖顺华; 赵青

    2015-01-01

    目的::探讨大麻素WIN55,212-2( WIN)对裸小鼠肝癌移植瘤及过氧化物酶体增殖物激活受体(PPARγ)、c-myc表达的影响。方法:采用5 mg/kg的WIN对荷瘤裸小鼠进行瘤周皮下注射干预15 d,每3天测量1次瘤体体重和体积,计算肿瘤体积和抑瘤率。荧光定量PCR和Western blotting测定HepG2移植瘤中PPARγ和c-myc的表达情况。结果:WIN对HepG2细胞移植瘤具有抑制作用,抑瘤率为66.00%,荧光定量PCR结果显示WIN抑制HepG2细胞移植瘤c-myc在mRNA水平的表达,促进HepG2细胞移植瘤PPARγ在mRNA水平表达。 Western blotting结果显示WIN抑制HepG2细胞移植瘤c-myc在蛋白水平的表达,促进HepG2细胞移植瘤PPARγ在蛋白水平表达。结论:WIN能够抑制HepG2细胞移植瘤的生长和c-myc的表达,并上调PPARγ的表达。%Objective:To investigate the effect of a cannabinoid, WIN55, 212-2 ( WIN ) , on HepG2 hepatocellular carcinoma xenografts and expression of peroxisome proliferators activated receptor ( PPAR-γ) and c-myc in nude mice. Methods :The tumor-bearing nude mice were injected subcutaneously around the tumor sites with 5 mg/kg WIN for 15 d. The weight and size of the tumor were measure every 3d for calculation of the tumor volume and tumor inhibition rate. PCR and western blotting assay were used to measure the expression of PPAR-γ and of c-myc in HepG2 tumor xenograft. Results:WIN showed inhibitory effect on the growth of HepG2 tumor xenograft, with an inhibition rate being 66.00%. Fluorescence quantitative PCR showed that WIN inhibited the expression of c-myc mRNA and promoted the expression of PPAR-γ mRNA in HepG2 tumor xenografts. Western blotting analysis showed that WIN inhibited the expression of c-myc proteins and promoted the expression of PPAR-γ proteins in HepG2 tumor xenografts. Conclusion:WIN may inhibit the growth of HepG2 tumor xenograft and c-myc expression, and upregulate the expression of PPAR-γ.

  12. CB1 Cannabinoid Agonist (WIN55,212-2) Within the Basolateral Amygdala Induced Sensitization to Morphine and Increased the Level of μ-Opioid Receptor and c-fos in the Nucleus Accumbens.

    Science.gov (United States)

    Molaei, Marzieh; Fatahi, Zahra; Zaringhalam, Jalal; Haghparast, Abbas

    2016-04-01

    The basolateral amygdala (BLA) is rich of CB1 cannabinoid receptors (CB1R) and has reciprocal connections with the nucleus accumbens (NAc) which is involved in opioid sensitization. In this study, effects of intra-BLA administration of CB1R agonist on sensitization to antinociceptive effect of morphine and changes in the levels of μ-opioid receptor (MOR), p-CREB, and c-fos in the NAc were investigated. Animals received intra-BLA microinjection of CB1R agonist (WIN55,212-2) once daily for 3 days consecutively (sensitization period). After 5 days free of drug, tail-flick test was performed before and after the administration of an ineffective dose of morphine. Afterward, the levels of MOR, p-CREB, and c-fos proteins were measured in the NAc by Western blot analysis. The results indicated that intra-BLA injection of WIN55,212-2 during sensitization period resulted in the induction of antinociceptive responses by ineffective dose of morphine and caused a significant increase in the MOR and c-fos levels but not p-CREB/CREB ratio in the NAc. These finding revealed that CB1 receptor agonist in the BLA induces development of morphine sensitization and increases expression of MOR in the NAc. It seems that c-fos is one of the important factors involved in the induction of sensitization to antinociceptive effect of morphine.

  13. An investigation on the effect of second-order additional thickness distributions to the upper surface of an NACA 64 sub 1-212 airfoil. [using flow equations and a CDC 7600 digital computer

    Science.gov (United States)

    Hague, D. S.; Merz, A. W.

    1975-01-01

    An investigation was conducted on a CDC 7600 digital computer to determine the effects of additional thickness distributions to the upper surface of an NACA 64 sub 1 - 212 airfoil. Additional thickness distributions employed were in the form of two second-order polynomial arcs which have a specified thickness at a given chordwise location. The forward arc disappears at the airfoil leading edge, the aft arc disappears at the airfoil trailing edge. At the juncture of the two arcs, x = x, continuity of slope is maintained. The effect of varying the maximum additional thickness and its chordwise location on airfoil lift coefficient, pitching moment, and pressure distribution was investigated. Results were obtained at a Mach number of 0.2 with an angle-of-attack of 6 degrees on the basic NACA 64 sub 1 - 212 airfoil, and all calculations employ the full potential flow equations for two dimensional flow. The relaxation method of Jameson was employed for solution of the potential flow equations.

  14. Diseño de un modelo para la optimización de los procesos en el mantenimiento programado aplicado al equipo Casa 212 de la Fuerza Aérea Colombiana

    Directory of Open Access Journals (Sweden)

    José Enrique Sarmiento

    2006-01-01

    Full Text Available Este proyecto es el resultado de Ia busqueda constante de un mejoramiento continuo de los procesos que garantizan la calidad total en el mantenimiento aeronautico, concebdo y desarrollado para ser aplicado en el mantenimiento basico de 100 horas o 1A del equipo C-212-300 asignado al grupo técnico del Comando Aéreo de Combate No.2. Es así como se hace un recuento acerca de Ia historia y Ia evolución del mantenimiento aeronautico, Ia normatividad y los metodos de trabajo, el estudio de tiempos y movimientos empleados actualmente, para garantizar Ia eficiencia y seguridad en Ia aviación comercial y militar. De ahí, que se visitó la Base Aerea del Ejercito del Aire Español, SATENA y la Base Aerea de Apiay de Ia Fuerza Aerea Colombiana, quienes t ienen dentro de sus flotas el equipo C-212.

  15. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228

    Energy Technology Data Exchange (ETDEWEB)

    Aleissa, Khalid A.; Almasoud, Fahad I.; Islam, Mohammed S. [Atomic Energy Research Institute, King Abdul Aziz City for Science and Technology, P.O. Box 6086, Riyadh 11442 (Saudi Arabia); L' Annunziata, Michael F. [IAEA Expert, Montague Group, P.O. Box 5033, Oceanside, CA 92052-5033 (United States)], E-mail: mlannunziata@cox.net

    2008-12-15

    The activities of {sup 228}Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide {sup 228}Ac. The radium was pre-concentrated on MnO{sub 2} and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter {sup 228}Ra({sup 228}Ac), the daughter nuclide {sup 228}Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by {sup 228}Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9{+-}0.1% was measured for {sup 228}Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317{+-}0.013 cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1 g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of {sup 228}Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for {sup 228}Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure {sup 228}Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  16. Radium-228 analysis of natural waters by Cherenkov counting of Actinium-228.

    Science.gov (United States)

    Aleissa, Khalid A; Almasoud, Fahad I; Islam, Mohammed S; L'Annunziata, Michael F

    2008-12-01

    The activities of (228)Ra in natural waters were determined by the Cherenkov counting of the daughter nuclide (228)Ac. The radium was pre-concentrated on MnO(2) and the radium purified via ion exchange and, after a 2-day period of incubation to allow for secular equilibrium between the parent-daughter (228)Ra((228)Ac), the daughter nuclide (228)Ac was isolated by ion exchange according to the method of Nour et al. [2004. Radium-228 determination of natural waters via concentration on manganese dioxide and separation using Diphonix ion exchange resin. Appl. Radiat. Isot. 61, 1173-1178]. The Cherenkov photons produced by (228)Ac were counted directly without the addition of any scintillation reagents. The optimum Cherenkov counting window, sample volume, and vial type were determined experimentally to achieve optimum Cherenkov photon detection efficiency and lowest background count rates. An optimum detection efficiency of 10.9+/-0.1% was measured for (228)Ac by Cherenkov counting with a very low Cherenkov photon background of 0.317+/-0.013cpm. The addition of sodium salicylate into the sample counting vial at a concentration of 0.1g/mL yielded a more than 3-fold increase in the Cherenkov detection efficiency of (228)Ac to 38%. Tests of the Cherenkov counting technique were conducted with several water standards of known activity and the results obtained compared closely with a conventional liquid scintillation counting technique. The advantages and disadvantages of Cherenkov counting compared to liquid scintillation counting methods are discussed. Advantages include much lower Cherenkov background count rates and consequently lower minimal detectable activities for (228)Ra and no need for expensive environmentally unfriendly liquid scintillation cocktails. The disadvantages of the Cherenkov counting method include the need to measure (228)Ac Cherenkov photon detection efficiency and optimum Cherenkov counting volume, which are not at all required when liquid scintillation analysis is used.

  17. Purification of selenium from thorium, uranium, radium, actinium and potassium impurities for low background measurements

    Energy Technology Data Exchange (ETDEWEB)

    Rakhimov, A.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz); Warot, G. [CEA-CNRS, Modane (France). Laboratoire Souterrain de Modane (LSM); Karaivanov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Institute for Nuclear Research and Nuclear Energy (INRNE), Sofia (Bulgaria); Kochetov, O.I.; Lebedev, N.A.; Filosofov, D.V. [Joint Institute for Nuclear Research (JINR), Dubna (Russian Federation); Mukhamedshina, N.M.; Sadikov, I.I. [Uzbek Academy of Sciences, Tashkent (Uzbekistan). Inst. of Nuclear Physics (INP AS RUz)

    2013-07-01

    A technique of selenium purification from {sup 232}Th, {sup 238}U, {sup 226,228}Ra, {sup 227}Ac and {sup 40}K was developed. This technique is simple to perform and employs a minimum number of highly pure reagents (bidistilled water, nitric acid). Operations carried out during purification (elution, evaporation) practically exclude losses of the target product (chemical yields of Se > 99%). A test purification of 100 g of selenium was carried out using this technique. The efficiency of this technique was confirmed by low background gamma spectrometry of the purified selenium sample. Distribution coefficients of Th, U, Ra and Ac on DOWEX 50W- x 8 cation-exchange resin at different concentrations of selenium and nitric acid were experimentally determinated. Instrumental neutron activation analysis of bidistilled water, deionized water and nitric acid was performed. (orig.)

  18. The release of dissolved actinium to the ocean: A global comparison of different end-members

    Science.gov (United States)

    Geibert, W.; Charette, M.; Kim, G.; Moore, W.S.; Street, J.; Young, M.; Paytan, A.

    2008-01-01

    The measurement of short-lived 223Ra often involves a second measurement for supported activities, which represents 227Ac in the sample. Here we exploit this fact, presenting a set of 284 values on the oceanic distribution of 227Ac, which was collected when analyzing water samples for short-lived radium isotopes by the radium delayed coincidence counting system. The present work compiles 227Ac data from coastal regions all over the northern hemisphere, including values from ground water, from estuaries and lagoons, and from marine end-members. Deep-sea samples from a continental slope off Puerto Rico and from an active vent site near Hawaii complete the overview of 227Ac near its potential sources. The average 227Ac activities of nearshore marine end-members range from 0.4??dpm m- 3 at the Gulf of Mexico to 3.0??dpm m- 3 in the coastal waters of the Korean Strait. In analogy to 228Ra, we find the extension of adjacent shelf regions to play a substantial role for 227Ac activities, although less pronounced than for radium, due to its weaker shelf source. Based on previously published values, we calculate an open ocean 227Ac inventory of 1.35 * 1018??dpm 227Acex in the ocean, which corresponds to 37??moles, or 8.4??kg. This implies a flux of 127??dpm m-2 y- 1 from the deep-sea floor. For the shelf regions, we obtain a global inventory of 227Ac of 4.5 * 1015??dpm, which cannot be converted directly into a flux value, as the regional loss term of 227Ac to the open ocean would have to be included. Ac has so far been considered to behave similarly to Ra in the marine environment, with the exception of a strong Ac source in the deep-sea due to 231Paex. Here, we present evidence of geochemical differences between Ac, which is retained in a warm vent system, and Ra, which is readily released [Moore, W.S., Ussler, W. and Paull, C.K., 2008-this issue. Short-lived radium isotopes in the Hawaiian margin: Evidence for large fluid fluxes through the Puna Ridge. Marine Chemistry]. Another potential mechanism of producing deviations in 227Ac/228Ra and daughter isotope ratios from the expected production value of lithogenic material is observed at reducing environments, where enrichment in uranium may occur. The presented data here may serve as a reference for including 227Ac in circulation models, and the overview provides values for some end-members that contribute to the global Ac distribution. ?? 2007 Elsevier B.V. All rights reserved.

  19. Synthesis, characterization and antimicrobial activity of novel platinum(IV) and palladium(II) complexes with meso-1,2-diphenyl-ethylenediamine-N,N‧-di-3-propanoic acid - Crystal structure of H2-1,2-dpheddp·2HCl·H2O

    Science.gov (United States)

    Radić, Gordana P.; Glođović, Verica V.; Ratković, Zoran R.; Novaković, Slađana B.; Garcia-Granda, Santiago; Roces, Laura; Menéndez-Taboada, Laura; Radojević, Ivana D.; Stefanović, Olgica D.; Čomić, Ljiljana R.; Trifunović, Srećko R.

    2012-12-01

    In the reaction of meso-1,2-diphenyl-ethylenediamine (1,2-dphen) with neutralized 3-chlor-propanoic acid, the new linear tetradentate edda-like ligand (edda = ethylenediamine-N,N'-diacetic ion) meso-1,2-diphenyl-ethylenediamine-N,N'-di-3-propanoic acid dihydrochloride monohydrate (H2-1,2-dpheddp·2HCl·H2O) was prepared. The corresponding platinum(IV) complex, s-cis-dichlorido-(meso-1,2-diphenyl-ethylenediamine-N,N'-di-3-propanoate)-platinum(IV) ([PtCl2(1,2-dpheddp)]) was synthesized by heating potassium-hexachloridoplatinate(IV) and H2-1,2-dpheddp·2HCl·H2O on steam bath for 12 h with neutralization by means of lithium-hydroxide. The palladium(II) complex, cis-dichlorido-(meso-1,2-diphenyl-ethylenediamine-N,N'-di-3-propanoate)-palladium(II) ([PdCl2(1,2-dpheddp)]) was obtained in the similar way using potassium-tetrachloridopalladate(II), H2-1,2-dpheddp·2HCl·H2O and lithium-hydroxide. The compounds were characterized by elemental analysis and infrared spectroscopy. The spectroscopically predicted structure of the synthesized tetradentate ligand was confirmed by X-ray analysis of the H2-1,2-dpheddp·2HCl·H2O. Antimicrobial activity of the ligand and corresponding palladium(II) and platinum(IV) complexes is investigated against 25 species of microorganisms. Testing is preformed by microdilution method and minimum inhibitory concentrations (MIC) and minimum microbicidal concentration (MMC) have been determined. The difference between antimicrobial activity of the ligand and corresponding platinum(IV) and palladium(II) complex is noticed and, in general, palladium(II) complex was the most active.

  20. Renal cell carcinoma with t(6 ;11)(p21.2;q13)/MALAT1-TFEB fusion: a clinical and pathological analysis%t(6;11)(p21.2;q13)/MALAT1-TFEB基因易位相关性肾癌的临床病理分析

    Institute of Scientific and Technical Information of China (English)

    夏秋媛; 时姗姗; 沈勤; 魏雪; 王璇; 马恒辉; 陆珍凤; 周晓军; 饶秋

    2015-01-01

    目的 探讨t (6; 11)(p21.2; q13)/MALAT1-TFEB基因易位相关性肾癌的临床病理特征、免疫表型、鉴别诊断及预后.方法 对9例TFEB基因易位相关性肾癌进行光镜观察、免疫组织化学EnVision法、荧光原位杂交、MALAT1-TFEB融合基因扩增研究及随访,并复习相关文献.结果 9例肿瘤中女性4例,男性5例.患者年龄21~42岁,平均31.3岁,平均随访31个月,均无病生存.镜下观察4例具特征性“假菊形团”结构,其余5例形态不典型,分别与血管周上皮样细胞肿瘤、肾透明细胞癌、嫌色细胞癌、乳头状肾细胞癌形态学有交叉.肿瘤内时常可见到基底膜样物质,色素及沙砾体.9例TFEB均呈弥漫细胞核表达,E-cadherin、波形蛋白一致强阳性,Ksp-cadherin、CD117阳性比例分别为7/9和6/9,色素性标志物Cathepsin K、HMB45和Melan A的阳性比例分别为8/9、6/9和6/9,广谱细胞角蛋白仅在1例中阳性,TFE3、CD10和CK7均阴性.荧光原位杂交9例均存在异常TFEB分离信号及MALAT1-TFEB融合信号.2例扩增出MALAT1-TFEB融合基因.结论 TFEB基因易位相关性肾癌是一种罕见肿瘤,好发于青壮年,预后较好.诊断可依据患者的年龄、病理学形态和免疫表型,荧光原位杂交检测TFEB基因易位是诊断该肿瘤的金标准.%Objective To study the clinicopathologic features,immunophenotype,differential diagnosis and prognosis of renal cell carcinoma (RCC) associated with t (6 ; 11) (p21.2 ; q13)/MALAT1-TFEB gene fusion.Methods A total of 9 cases of such rare tumor were selected for clinicopathologic,immunohistochemical and molecular analysis,with review of literature.Results The age of the patients ranged from 21 to 42 years (mean =31.3 years).The patients included four men and five women.Histologically,4 of the 9 cases studied showed classic morphologic features of TFEB RCC,with hyaline material,pigments and psammoma bodies frequently identified.The remaining 5 cases demonstrated

  1. An Initial Investigation of the Confidence Intervals for Thyroid Hormone Levels of the Infants Between the Age ;of 2-1 2 Weeks%2~12周龄婴儿甲状腺激素水平初探

    Institute of Scientific and Technical Information of China (English)

    王洁; 裘蕾; 孔元原

    2014-01-01

    目的:探讨首都医科大学附属北京妇产医院2~12周龄婴儿甲状腺激素水平的参考区间。方法选取2009年1月至2012年12月于北京市新生儿疾病筛查门诊就诊的2~12周龄的953例健康婴儿为研究对象。按照不同年龄段,将其分为2~4周龄组(n=416)、4~8周龄组(n=398)和8~12周龄组(n=139)。3组受试儿童的性别、胎龄、出生体质量等一般临床资料比较,差异无统计学意义(P>0.05)。应用化学发光法测定血清促甲状腺素(TSH)、三碘甲状腺原氨酸(TT3)、四碘甲状腺原氨酸(TT4)、游离三碘甲状腺原氨酸(FT3)、游离四碘甲状腺原氨酸(FT4)的水平,各指标参考区间采用中位数及 P2.5、P25、P50、P75、P97.5表示,同时对不同性别、不同年龄组间的甲状腺激素水平,及婴儿与成年人甲状腺激素水平参考区间进行统计学分析(本研究遵循的程序符合首都医科大学附属北京妇产医院人体试验委员会制定的伦理学标准,征得该委员会批准,并征得受试对象监护人的知情同意,与之签署临床研究知情同意书)。结果 TSH、FT3、FT4、TT3、TT4水平男性与女性婴儿间比较,差异均无统计学意义(t=1.860,0.510,-0.360,0.920,1.370;P>0.05)。2~12周龄婴儿各激素水平与目前本院应用的成年人甲状腺激素水平参考区间进行比较,TSH、FT3、T3、T4水平高于成年人参考区间,且差异均有统计学意义(t=27.25,46.77,30.49,5.59;P0.05).Thyroid hormone levels (TSH,FT3,TT3,TT4)of children at the age of 2-12 weeks were higher than those of adults,with significant differences (t=27.25,46.77, 30.49,5.59,P=0.000).Conclusions The reference intervals of adults are not universally applicable to infants.It is important to establish the reference intervals of thyroid hormone levels of infants.

  2. Study on detection and treatment of cervical lesions in 10 212 women in Yingcheng%应城市10212例妇女宫颈病变检测及治疗的研究

    Institute of Scientific and Technical Information of China (English)

    刘永桥; 李艳; 杨丽

    2012-01-01

    Objective; To detect, diagnose, and evaluate cervical lesions, especially the degree and state of cervical precancerous lesion by liquid - based thin layer cytological test ( TCT) combined with biopsy, provide an accurate reference for treating cervical lesions reasonably in clinic, observe the curative effect. Methods; A total of 10 212 women were detected by TCT for successive three years, including 457 female workers from the hospital. Cytological diagnosis was made according to TBS criteria. Partial women with positive TCT results received cervical biopsy. Results; Among 10 212 women, 391 women were found with positive cytological results (above ASC - US) , the positive rate was 3. 83% , including 296 women with ASC - US (2. 90% ) , 85 women with ASC - H ( 0. 84% ) , 5 women with LSIU (0. 05% ) , 3 women with HSIL (0. 03% ) , one woman with SCC (0. 01% ) , and one woman with AGC (0. 01% ) . The positive rate of cytologieal screening in 457 female workers in the hospital for successive three years was 3.42% , 0. 88% , and 0.00% , respectively. Conclusion: TCT has distinct advantages for cervical lesions screening and curative effect observation after gynecological treatment , TCT combined with cervical biopsy and treatment can reduce the occurrence and development of cervical lesions, which has important clinical application value.%目的:采用液基薄层细胞制片技术(TCT)结合组织活检术检测、诊断和评价宫颈病变,特别是癌前病变的程度及所处的状态,为临床合理治疗宫颈病变提供准确依据,观察治疗后的效果.方法:运用TCT连续3年检测了10 212例妇女,包括457例应城市人民医院女职工.细胞学诊断按TBS标准.对TCT部分阳性者进行宫颈活检.结果:10 212例妇女中,细胞学阳性(ASC-UC以上)391例,阳性率为3.83%,其中ASC-UC 296例(2.90%)、ASC-H 85例(0.84%)、LSIL 5例(0.05%)、HSIL 3例(0.03%)、SCC 1例(0.01%)、AGC 1例(0.01%).应城市人民医院457

  3. The analysis of the risk factors for sacroiliac joint damage in juvenile-onset spondyloarthropathy%212例少年脊柱关节病骶髂关节损害相关危险因素分析

    Institute of Scientific and Technical Information of China (English)

    单鸣凤; 郭君; 陈鸿健

    2012-01-01

    目的:探讨少年脊柱关节病(juvenile-onset spondyloarthropathy,JSpA)患者出现骶髂关节损害的相关危险因素,为选择恰当的治疗方案提供依据.方法:对212例JSpA患者的人口学因素、遗传学因素、受累部位、各个实验室指标等进行分析.将患者按照年龄段(≤11岁及12~16岁)分为两组.分别采用Logistic回归的方法进行分析,筛选出JSpA出现骶髂关节损害的相关发病因素.结果:本组212例患者中有骶髂关节损害者124例,占58.5%.单因素分析显示多个因素在骶髂关节损害中有统计学意义;多因素Logistic逐步回归分析显示病程、起病时外周关节炎尤其是下肢大关节、持续肌腱端炎、HLA-B27阳性、家族史是JSpA并发骶髂关节损害的相关危险因素.结论:JSpA患者出现骶髂关节损害的发生率较高,与多种因素有关,当患者出现这些危险因素时,应早期积极干预治疗,定期检查骶髂关节MRI,减轻或延缓病情的进展.%Objective:To investigate the risk factors of sacroiliac joint damage in patients with juvenile-onset spondyloarthropathy (JSpA),and to provide reference for the selection of appropriate treatment strategy. Methods:To analysis the clinical data of 212 cases of JSpA,including demographic factors(sex,age) .genetic factors,the affected area,laboratories indicators. Patients were divided into two groups according to age (≤ 11 years old and 12~16 years old). Using the Logistic regression to filter out the risk factors of sacroiliac joint damage in patients with JSpA. Results:Out of the 212 cases, 124 (58.5%) patients suffered from sacroiliac joint damage. Single factor analysis showed that a number of factors were related to sacroiliac joint damage. Logistic regression analysis showed that the course of disease, at the onset of peripheral arthritis, particularly the large joints of lower limbs, tendon-sustained inflammation and HLA-B27-positive, family history were the risk

  4. Analysis of 212 cases of patients on Guizhou Province with psoriasis vulgaris pathogenesis characteristics%贵州省寻常型银屑病患者发病特征分析

    Institute of Scientific and Technical Information of China (English)

    盛良; 姚学群; 宋守荣; 魏羽佳; 刘素琴; 廖跃

    2013-01-01

    Objective To catch on onset feature on Guizhou Province in familial and sporadic pso-riasis vulgaris (PV ) by analyzing the psoriasis vulgaris ,then compares onset age ,disease severity ,af-fected sites ,onset season and other inducts factors in different features between in familial and sporad-ic Psoriasis Vulgaris .Methods 212 patients with PV were enrolled from dermatology outpatient clinic of Affiliated Hospital of Guiyang Medical College between March 2011 and April 2012 .There were 212 patients which were 35 familial and 177 sporadic patients .Results 212 patients (123 men and 89 woman) ,male and female sex ratio was 1 .41 :1 ,Mean visit age was 37 .60 ± 14 .46 ,Mean outbreak age was 27 .75 ± 13 .40 ,PV patients could be seen in every education background and occupation .35 familial(16 .51) and 177 sporadic (83 .49)patients ,Familial PV patients had earlier onset age (t=4 .260 ,P<0 .001) and milder clinical manifestation(χ2 =7 .877 ,P=0 .019)than sporadic PV patients . There were no statistical significantly differences sbetween familial PV and sporadic P in the diseased parts V .Compared with familial PV ,more sporadic PV had histories of infection (χ2 =3 .930 ,P=0 .047) ,trauma (χ2 =6 .462 ,P=0 .011) .Conclusion From the analysis of our data ,it seems that fa-milial and sporadic PV have significant differences in onset age ,disease severity ,onset sites and asso-ciated inducts factors .There may be related to different pathogenesis in familial and sporadic PV .%目的通过对贵州省寻常型银屑病(PV )的调查分析,了解有家族史及无家族史PV患者的发病特征,比较其两者在首发年龄、发病季节、病情程度、发病部位、发病前诱因等发病特征上的不同。方法收集212例寻常型PV患者的临床资料(有家族史35例,无家族史177例)进行统计分析。结果212例PV患者中男性123例,女性89例,男女性别比1.38:1。平均就诊年龄(37.60±14.46

  5. Effect of morphology and manganese valence on the voltage fade and capacity retention of Li[Li2/12Ni3/12Mn7/12]O2.

    Science.gov (United States)

    Verde, Michael G; Liu, Haodong; Carroll, Kyler J; Baggetto, Loïc; Veith, Gabriel M; Meng, Y Shirley

    2014-11-12

    We have determined the electrochemical characteristics of the high voltage, high capacity Li-ion battery cathode material Li[Li2/12Ni3/12Mn7/12]O2 prepared using three different synthesis routes: sol-gel, hydroxide coprecipitation, and carbonate coprecipitation. Each route leads to distinct morphologies and surface areas while maintaining the same crystal structures. X-ray photoelectron spectroscopy (XPS) measurements reveal differences in their surface chemistries upon cycling, which correlate with voltage fading. Indeed, we observe the valence state of Mn on the surface to decrease upon lithiation, and this reduction is specifically correlated to discharging below 3.6 V. Furthermore, the data shows a correlation of the formation of Li2CO3 with the Mn oxidation state from the decomposition of electrolyte. These phenomena are related to each material's electrochemistry in order to expand upon the reaction mechanisms taking place-specifically in terms of the particle morphology produced by each synthetic approach.

  6. Localization of the genetic locus for Saethre-Chotzen syndrome to a 6 cM region of chromosome 7 using four cases with apparently balanced translocations at 7p21.2.

    Science.gov (United States)

    Rose, C S; King, A A; Summers, D; Palmer, R; Yang, S; Wilkie, A O; Reardon, W; Malcolm, S; Winter, R M

    1994-08-01

    Saethre-Chotzen syndrome is a common autosomal dominant form of craniosynostosis, which results in the premature fusion of cranial sutures. Craniosynostosis is commonly associated with abnormalities of 7p; Vortkamp et al. (Nature 352, 539-540) demonstrated that the GLI3 gene in 7p13 was disrupted in, patients with Greig syndrome and, more recently, the linkage of genetic markers from 7p with the Saethre-Chotzen syndrome locus has been reported (2,3). Here we report the analysis by fluorescence in situ hybridization of four patients with Saethre-Chotzen syndrome associated with apparently balanced translocations involving band 7p21.2 and different reciprocal chromosomes. We show that in all four patients the breakpoints in 7p are situated within a 6 cM region flanked by the genetic markers D7S488 and D7S493. These results provide further evidence that the genetic locus for Saethre-Chotzen syndrome is located in distal 7p.

  7. A de novo balanced translocation t(7;12)(p21.2;p12.3) in a patient with Saethre-Chotzen-like phenotype downregulates TWIST and an osteoclastic protein-tyrosine phosphatase, PTP-oc.

    Science.gov (United States)

    De Marco, Patrizia; Raso, Alessandro; Beri, Silvana; Gimelli, Stefania; Merello, Elisa; Mascelli, Samantha; Baldi, Maurizia; Baffico, Ave Maria; Pavanello, Marco; Cama, Armando; Capra, Valeria; Giorda, Roberto; Gimelli, Giorgio

    2011-01-01

    Saethre-Chotzen syndrome (SCS) is an autosomal dominant craniosynostosis syndrome with variable expression. Here we report on a female infant with a de novo balanced translocation 46, XX, t(7;12)(p21.2;p12.3) and presenting at birth brachycephaly, antimongolic palpebral fissures, ocular hypertelorism, broad nose with low nasal bridge and low-set ears. This phenotype is suggestive of a subtle form of SCS, given the absence of limbs anomalies. Cloning of both breakpoints revealed that the translocation does not interrupt the TWIST1 coding region, on 7p21, known to be causative for SCS, but downregulates TWIST1 expression due to a position effect. On chromosome 12, the breakpoint translocates a shorter transcript of PTPRO gene, the osteoclastic protein-tyrosine phosphatase, PTP-oc, near to regulatory region of 7p leading to down-regulation of PTP-oc in the proband's fibroblasts. This is a confirmatory case report providing further evidence for TWIST1 haploinsufficiency in SCS, although a possible role of PTP-oc as genetic factor underlying or at least influencing the development of craniosynostosis could not be a priori excluded.

  8. Effects of WIN 55,212-2 (a synthetic cannabinoid CB1 and CB2 receptor agonist) on the anticonvulsant activity of various novel antiepileptic drugs against 6 Hz-induced psychomotor seizures in mice.

    Science.gov (United States)

    Florek-Luszczki, Magdalena; Wlaz, Aleksandra; Zagaja, Mirosław; Andres-Mach, Marta; Kondrat-Wrobel, Maria W; Luszczki, Jarogniew J

    2015-03-01

    The purpose of this study was to determine the influence of WIN 55,212-2 mesylate (WIN-a non-selective cannabinoid CB1 and CB2 receptor agonist) on the anticonvulsant activity of various second- and third-generation antiepileptic drugs (i.e., gabapentin, lacosamide, levetiracetam, oxcarbazepine, pregabalin and tiagabine) in the mouse 6 Hz-induced psychomotor seizure model. Psychomotor seizures were evoked in albino Swiss mice by a current (32 mA, 6 Hz, 3s stimulus duration) delivered via ocular electrodes. Additionally, total brain antiepileptic drug concentrations were measured. Results indicate that WIN (5 mg/kg, administered i.p.) significantly potentiated the anticonvulsant action of gabapentin (P anticonvulsant activity of all tested antiepileptic drugs in the 6 Hz test in mice. Measurement of total brain antiepileptic drug concentrations revealed that WIN (5 mg/kg) had no impact on gabapentin or levetiracetam total brain concentrations, indicating the pharmacodynamic nature of interaction between these antiepileptic drugs in the mouse 6Hz model. In conclusion, WIN in combination with gabapentin and levetiracetam exerts beneficial anticonvulsant pharmacodynamic interactions in the mouse psychomotor seizure model. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. Aespoe Hard Rock Laboratory. Aespoe Task Force on Engineered Barrier System. Modelling of THM-coupled processes for benchmark 2.1.1 and 2.1.2 with the code GeoSys/RockFlow

    Energy Technology Data Exchange (ETDEWEB)

    Nowak, Thomas; Kunz, Herbert (Federal Inst. for Geosciences and Natural Resources, Hannover (Germany))

    2009-03-15

    In 2004 the Swedish Nuclear Fuel and Waste Management Co. (SKB) initiated the project 'Task Force on Engineered Barrier Systems'. This project has the objectives to verify the ability to model THM-coupled processes (task 1) and gas migration processes (task 2) in the clay-rich buffer materials. The tasks are performed on the basis of appropriate benchmarks. This report describes the final results for the modelling of the THM-benchmarks 2.1.1(Buffer/Container Experiment) and 2.1.2 (Isothermal Test) with the code GeoSys/RockFlow. Both in-situ experiments were conducted in the Canadian Whiteshell Underground Research Laboratory (URL). An interim report (NOWAK 2008) documented the results of two axially symmetric models. This report documents the calculations of a laboratory experiment on the buffer material that is used as calibration for the hydraulic properties of the buffer material, the setting-up of a 3-D model of the URL for both ITT and BCE and the modelling results in comparison to measured values.

  10. The effect of WIN 55,212-2 suggests a cannabinoid-sensitive component in the early toxicity induced by organic acids accumulating in glutaric acidemia type I and in related disorders of propionate metabolism in rat brain synaptosomes.

    Science.gov (United States)

    Colín-González, A L; Paz-Loyola, A L; Serratos, I N; Seminotti, B; Ribeiro, C A J; Leipnitz, G; Souza, D O; Wajner, M; Santamaría, A

    2015-12-01

    Several physiological processes in the CNS are regulated by the endocannabinoid system (ECS). Cannabinoid receptors (CBr) and CBr agonists have been involved in the modulation of the N-methyl-D-aspartate receptor (NMDAr) activation. Glutaric (GA), 3-hydroxyglutaric (3-OHGA), methylmalonic (MMA) and propionic (PA) acids are endogenous metabolites produced and accumulated in the brain of children affected by severe organic acidemias (OAs) with neurodegeneration. Oxidative stress and excitotoxicity have been involved in the toxic pattern exerted by these organic acids. Studying the early pattern of toxicity exerted by these metabolites is crucial to explain the extent of damage that they can produce in the brain. Herein, we investigated the effects of the synthetic CBr agonist WIN 55,212-2 (WIN) on early markers of GA-, 3-OHGA-, MMA- and PA-induced toxicity in brain synaptosomes from adult (90-day-old) and adolescent (30-day-old) rats. As pre-treatment, WIN exerted protective effects on the GA- and MMA-induced mitochondrial dysfunction, and prevented the reactive oxygen species (ROS) formation and lipid peroxidation induced by all metabolites. Our findings support a protective and modulatory role of cannabinoids in the early toxic events elicited by toxic metabolites involved in OAs.

  11. 8 CFR 212.4 - Applications for the exercise of discretion under section 212(d)(1) and 212(d)(3).

    Science.gov (United States)

    2010-01-01

    ... SECURITY IMMIGRATION REGULATIONS DOCUMENTARY REQUIREMENTS: NONIMMIGRANTS; WAIVERS; ADMISSION OF CERTAIN... application a written statement of the history of the applicant's membership or affiliation, including...

  12. Non-Canonical Processing of Arabidopsis pri-miR319a/b/c Generates Additional microRNAs to Target One RAP2.12 mRNA Isoform.

    Science.gov (United States)

    Sobkowiak, Lukasz; Karlowski, Wojciech; Jarmolowski, Artur; Szweykowska-Kulinska, Zofia

    2012-01-01

    Arabidopsis miR319a/b/c primary transcripts are unusual due to the presence of a long stem and loop structure containing functional miR319a/b/c molecules. In our experiments carried out using high throughput sequencing (HTS), we have shown that additional microRNAs (miRNAs), miR319a.2/b.2/c.2 are generated from the upper part of the same hairpin structure. We have also found cognate miRNAa.2*/b.2*/c.2* to be present in the HTS results with a considerably lower number of reads. Northern hybridization revealed that miR319b.2 is mainly expressed in 35-day-old plant rosette leaves, as well as in stem and inflorescences of 42- and 53-day-old plants. Moreover, it carries multiple signatures of a functional miRNA, including as follows: (i) its biogenesis is HYL1-dependent; (ii) it is incorporated in a substantial amount into RISC complexes containing AGO1, AGO2, or AGO4 protein; (iii) 24 nt-long species of miR319b.2 have been found in inflorescences to be more abundant than 21 nt miR319b.2 species; (iv) it is present in various ratios to miR319b during plant development, which suggests the existence of a regulatory mechanism responsible for its biogenesis/processing; (v) there is an observed cross-species conservation of the miR319a/b/c stem nucleotide sequence extending beyond mature miRNA region; and (vi) all evidence suggests that intron-containing RAP2.12 mRNA isoform is the target for miR319b.2. All these features prompt us to claim miR319b.2 as a functional miRNA molecule.

  13. A Spatially Resolved Study of Cold Dust, Molecular Gas, H ii Regions, and Stars in the z = 2.12 Submillimeter Galaxy ALESS67.1

    Science.gov (United States)

    Chen, Chian-Chou; Hodge, J. A.; Smail, Ian; Swinbank, A. M.; Walter, Fabian; Simpson, J. M.; Calistro Rivera, Gabriela; Bertoldi, F.; Brandt, W. N.; Chapman, S. C.; da Cunha, Elisabete; Dannerbauer, H.; De Breuck, C.; Harrison, C. M.; Ivison, R. J.; Karim, A.; Knudsen, K. K.; Wardlow, J. L.; Weiß, A.; van der Werf, P. P.

    2017-09-01

    We present detailed studies of a z = 2.12 submillimeter galaxy, ALESS67.1, using sub-arcsecond resolution ALMA, adaptive optics-aided VLT/SINFONI, and Hubble Space Telescope (HST)/CANDELS data to investigate the kinematics and spatial distributions of dust emission (870 μm continuum), 12CO(J = 3–2), strong optical emission lines, and visible stars. Dynamical modeling of the optical emission lines suggests that ALESS67.1 is not a pure rotating disk but a merger, consistent with the apparent tidal features revealed in the HST imaging. Our sub-arcsecond resolution data set allows us to measure half-light radii for all the tracers, and we find a factor of 4–6 smaller sizes in dust continuum compared to all the other tracers, including 12CO; also, ultraviolet (UV) and Hα emission are significantly offset from the dust continuum. The spatial mismatch between the UV continuum and the cold dust and gas reservoir supports the explanation that geometrical effects are responsible for the offset of the dusty galaxy on the IRX–β diagram. Using a dynamical method we derive an {α }{CO}=1.8+/- 1.0, consistent with other submillimeter galaxies (SMGs) that also have resolved CO and dust measurements. Assuming a single {α }{CO} value we also derive resolved gas and star formation rate surface densities, and find that the core region of the galaxy (≲ 5 kpc) follows the trend of mergers on the Schmidt–Kennicutt relationship, whereas the outskirts (≳ 5 kpc) lie on the locus of normal star-forming galaxies, suggesting different star formation efficiencies within one galaxy. Our results caution against using single size or morphology for different tracers of the star formation activity and gas content of galaxies, and therefore argue the need to use spatially resolved, multi-wavelength observations to interpret the properties of SMGs, and perhaps even for z> 1 galaxies in general.

  14. Effects of the CB1R agonist WIN-55,212-2 and the CB1R antagonists SR-141716 and AM-1387: open-field examination in rats.

    Science.gov (United States)

    Järbe, T U C; Ross, T; DiPatrizio, N V; Pandarinathan, L; Makriyannis, A

    2006-09-01

    This study examined the open-field (O-F) effects in rats of the cannabinoid 1 receptor (CB1R) agonist WIN-55,212-2 (WIN; 1 to 5.6 mg/kg) and its interaction with the CB1R antagonist/inverse agonist SR-141716 (1 to 5.6 mg/kg). Additionally, separate studies examined the O-F effects of SR-141716 (1 to 10 mg/kg) and a newly synthesized CB1R selective antagonist/inverse agonist AM-1387 (3 and 10 mg/kg) when these ligands were administered alone. Both antagonists are characterized in vitro by decreased of GTPgammaS binding and increased cAMP accumulation (inverse agonism). WIN dose dependently reduced ambulation (horizontal activity) and rearing (vertical activity); SR-141716 completely (WIN 3 mg/kg) or partially (WIN 5.6 mg/kg) normalized these behaviors. WIN alone resulted in circling and in an increased latency to leave the start area of the O-F, effects blocked by all doses of SR-141716. Both the increased scratching and grooming, associated with SR-141716 administration, were attenuated but not abolished by WIN. SR-141716 alone tended to reduce ambulation (significant at 10 mg/kg) and rearing (non-significant), had no effect on latency, and increased scratching and grooming (both frequency and duration), at doses of 3 mg/kg and up. At the doses examined, AM-1387 had no effect on ambulation, rearing, latency but significantly increased scratching (10 mg/kg); there was also a trend for increased grooming (both frequency and duration). The O-F profile of WIN suggests more similarity with the effects of THC rather than methanandamide (and presumably also anandamide). Intrinsic activity (scratching and grooming) by SR-141716 was re-affirmed and seemed to be associated with administration of AM-1387 as well. AM-1387 was less potent than SR-141716.

  15. Pharmacokinetic-pharmacodynamic influence of N-palmitoylethanolamine, arachidonyl-2'-chloroethylamide and WIN 55,212-2 on the anticonvulsant activity of antiepileptic drugs against audiogenic seizures in DBA/2 mice.

    Science.gov (United States)

    Citraro, Rita; Russo, Emilio; Leo, Antonio; Russo, Roberto; Avagliano, Carmen; Navarra, Michele; Calignano, Antonio; De Sarro, Giovambattista

    2016-11-15

    We evaluated the effects of ACEA (selective cannabinoid (CB)1 receptor agonist), WIN 55,212-2 mesylate (WIN; non-selective CB1 and CB2 receptor agonist) and N-palmitoylethanolamine (PEA; an endogenous fatty acid of ethanolamide) in DBA/2 mice, a genetic model of reflex audiogenic epilepsy. PEA, ACEA or WIN intraperitoneal (i.p.) administration decreased the severity of tonic-clonic seizures. We also studied the effects of PEA, WIN or ACEA after co-administration with NIDA-41020 (CB1 receptor antagonist) or GW6471 (PPAR-α antagonist) and compared the effects of WIN, ACEA and PEA in order to clarify their mechanisms of action. PEA has anticonvulsant features in DBA/2 mice mainly through PPAR-α and likely indirectly on CB1 receptors, whereas ACEA and WIN act through CB1 receptors. The co-administration of ineffective doses of ACEA, PEA and WIN with some antiepileptic drugs (AEDs) was examined in order to identify potential pharmacological interactions in DBA/2 mice. We found that PEA, ACEA and WIN co-administration potentiated the efficacy of carbamazepine, diazepam, felbamate, gabapentin, phenobarbital, topiramate and valproate and PEA only also that of oxcarbazepine and lamotrigine whereas, their co-administration with levetiracetam and phenytoin did not have effects. PEA, ACEA or WIN administration did not significantly influence the total plasma and brain levels of AEDs; therefore, it can be concluded that the observed potentiation was only of pharmacodynamic nature. In conclusion, PEA, ACEA and WIN show anticonvulsant effects in DBA/2 mice and potentiate the effects several AEDs suggesting a possible therapeutic relevance of these drugs and their mechanisms of action. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. 40 CFR 21.2 - Definitions.

    Science.gov (United States)

    2010-07-01

    ....S.C. 1151, et seq. (f) Pollutant means dredged spoil, solid waste, incinerator residue, sewage... section 510 of the Act; (5) Any requirements necessary to comply with an areawide management plan approved... Small Business Administration. (q) Areawide agency means an areawide management agency designated under...

  17. Calidad de Imagen del Telescopio UNAM212

    Science.gov (United States)

    Cobos, F. J.; Teiada de Vargas, C.

    1987-05-01

    El telescopio UNAM2l2, del Observatorio Astronómico Nacional, situado en la Sierra de San Pedro Mártir (Baja California, México), cumplira en un futuro muy cercano siete años de uso para fines de investigación astronómica. Aunque en este tiempo no se ha efectuado un estudio sistemático acerca de su comportamiento óptico y de los factores que influyen en la calidad de las imágenes, se han realizado pruebas diversas, estudios parciales y reuniones especificas, cuyos resultados no siempre se han difundido ampliamente y generalmente no se han presentado por escrito. Es por ello que hemos creido necesario intentar una recopilación de la información existente para poder con ella establecer un diagnóstjco que, aunque no sea definitivo, sirva de base para futuros trabajos tendientes a optimizar el comportamiento óptico del telescopio. Es evidente que un buen número de las conclusiones que se presentan son resultado del trabajo de muchas personas ó de esfuerzos colectivos. Asimismo, hemos tratado de localizar información bibliográfica que pueda ser de utilidad. Nuestro objetivo primordial ha consistido en centrarnos en la óptica del telescopio y su calidad, pero también se han considerado otros aspectos que puedan afectar las imágenes obtenidas tales como: celda del primario, `seeing' local y externo, flexiones posibles en la estructura mecánica del telescopio, etc.

  18. 36 CFR 212.1 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... Forest System. As defined in the Forest Rangeland Renewable Resources Planning Act, the “National Forest... protection, administration, and utilization of the National Forest System and the use and development of its resources. Forest transportation atlas. A display of the system of roads, trails, and airfields of an...

  19. 12 CFR 212.2 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... villages whose borders touch each other or whose borders are within 10 road miles of each other at their closest points. The property line of an office located in an unincorporated city, town, or village is the... million or more; (iii) A senior executive officer as that term is defined in 12 CFR 225.71(c); (iv)...

  20. 212 TRADITIONAL MEDICINES AND THEIR POTENTIAL ...

    African Journals Online (AJOL)

    Windows User

    derived from nature, including herbs, animal and mineral products. ... abortion, treatment of threatened abortion (Tang et al., 2012) or labor (Kamatenesi- ... Several plants are used as traditional medical therapies, Cissampelos .... women of childbearing age, particularly when it is known that they are trying to conceive or.

  1. 24 CFR 1710.212 - Financial information.

    Science.gov (United States)

    2010-04-01

    ...) Estimated marketing and advertising costs. (iv) Estimated sales commission. (v) Interest (include cost in... intended to prohibit the taking of an instrument as security for the lot purchase price after title is conveyed. For the purposes of these exceptions, these definitions shall apply: (i) “Deed” shall mean...

  2. 25 CFR 212.3 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... natural person, proprietorship, partnership, corporation, or other entity which has entered into a lease... existing lease; marketability; and potential environmental, social, and cultural effects. Indian lands..., clay or silt is the subject mineral, an enterprise is considered “mining” only if the extraction...

  3. 7 CFR 989.212 - Substandard dockage.

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... percent, by weight, of substandard raisins may be acquired by a handler under a weight dockage system. A..., by weight, of substandard raisins under a weight dockage system. The creditable weight of each lot...

  4. 29 CFR 1603.212 - Witness fees.

    Science.gov (United States)

    2010-07-01

    ... STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE... served, or, if the witness appears voluntarily, at the time of appearance. A federal agency...

  5. 32 CFR 212.6 - Procedures.

    Science.gov (United States)

    2010-07-01

    ....6 Procedures. (a) To prevent the appearance of official sanction or support by the Department of...-Federal entity is apparent and unambiguous and there is no appearance of official sanction or support by... discrimination by any non-Federal entity or other private organization covered by this part. (ii)...

  6. 12 CFR 212.6 - General exemption.

    Science.gov (United States)

    2010-01-01

    ... monopoly or substantial lessening of competition, and would not present safety and soundness concerns. (b... rebuttable presumption that an interlock will not result in a monopoly or substantial lessening of... continue so long as it does not result in a monopoly or substantial lessening of competition, or is...

  7. Reference: 212 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available identified in pea (Pisum sativum) using biochemical approaches. The Arabidopsis (...C75-IV, which we studied using a range of molecular, genetic, and biochemical techniques. Expression of atTO

  8. 45 CFR 212.10 - Nondiscrimination.

    Science.gov (United States)

    2010-10-01

    ... otherwise be subjected to discrimination of any nature or form in the provision of any benefits under the Act. (b) The prohibition in paragraph (a) of this section precludes discrimination either in...

  9. 42 CFR 2.12 - Applicability.

    Science.gov (United States)

    2010-10-01

    ... extends past that date) for the purpose of treating alcohol or drug abuse, making a diagnosis for that... that date), for the purpose of treating alcohol or drug abuse, making a diagnosis for the treatment, or... financial assistance in any form, including financial assistance which does not directly pay for the...

  10. 34 CFR 682.212 - Prohibited transactions.

    Science.gov (United States)

    2010-07-01

    ... (2) Induce a lender to make loans to either the students or the parents of students of a particular school or particular category of students or their parents. (b) The following are examples of... purposes. (c) Except when purchased by an agency of any State functioning as a secondary market or in...

  11. 47 CFR 15.212 - Modular transmitters.

    Science.gov (United States)

    2010-10-01

    ... modular transmitter must have their own shielding. The physical crystal and tuning capacitors may be... shielded. The physical crystal and tuning capacitors may be located external to the shielded radio...

  12. 21.PSYCHIATRIC DISORDER 21.2.Schizophrenia

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    920198 Kinetic observation of serum thyr-onormone level in schizophrenics.WANGXueyi (王学义),et al.Psychiatr Hosp,KailuanCoal Mining Administration,Tangshan,063001.Chin J Neurol & Psychiat 1991; 24 (5): 268-271.Measurements of serum T3,T4,RUR and FT4I

  13. PSYCHIATRIC DISORDER21.2.Schizophrenia

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    950421 A study on therapeutic efficacy,side effectsand correlation between plasma concentration andtherapeutic effect of haloperridol decanoate on treat-ment of schizophrenia.ZHANG Hongyan(张鸿燕),etal.Ment Health Instit,Beijing Med Univ,Beijing,100083.Chin J Neurol & Psychiat 1994;27(5):283-285.Haloperidol decanoate (HD) was conducted in treat-ing 83 schizophrenics in nine hospitals for 24 weeks,meanwhile plasma drug concentration was measured.The results show that therapeutic effectiveness of HDis saticfactory in more than 50%;the static plasmaconcentration in recovery and obviously improved pa-

  14. 43 CFR 17.212 - Employment criteria.

    Science.gov (United States)

    2010-10-01

    ..., manual, or speaking skills the test results accurately reflect the applicant's or employee's job skills... shown to be job-related for the position in question, and (2) alternative job-related tests or criteria... employee's impaired sensory, manual, or speaking skills (except where those skills are the factors that...

  15. 40 CFR 94.212 - Labeling.

    Science.gov (United States)

    2010-07-01

    ... manufacturer. (10) The application for which the engine family is certified. (For example: constant-speed auxiliary, variable-speed propulsion engines used with fixed-pitch propellers, recreational, etc.) (c) The... manufacturer in accordance with the applicable emission standards, including but not limited to idle speeds(s...

  16. 19 CFR 10.212 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... polymers, fibers, filaments, textile strips, yarns, twine, cordage, rope, or strips of fabric and ending..., starting with the extrusion of filament, strip, film, or sheet and including slitting a film or sheet...

  17. Structural analysis of HS(CD(2))(12)(O-CH(2)-CH(2))(6)OCH(3) monolayers on gold by means of polarization modulation infrared reflection absorption spectroscopy. progress of the reaction with bromine.

    Science.gov (United States)

    Brand, Izabella; Nullmeier, Martina; Klüner, Thorsten; Jogireddy, Rajamalleswaramma; Christoffers, Jens; Wittstock, Gunther

    2010-01-05

    A self-assembled monolayer (SAM) on gold was formed with specifically perdeuterated hexaethylene glycol-terminated alkanethiol HS(CD(2))(12)(O-CH(2)-CH(2))(6)OCH(3) (D-OEG). The structure of the d-alkane and the oligoethylene glycol (OEG) parts of the molecule in a SAM was studied by means of polarization modulation infrared reflection absorption spectroscopy. The D-OEG monolayers are highly ordered and exist in a crystalline phase. The d-alkane chain adopts an all-trans conformation. Both, the d-alkane chain and long axis of the OEG part make an angle of 26.0 degrees +/- 1.5 degrees with respect to the surface normal, a value characteristic for the tilt of solid n-alkane thiols in the SAMs on Au. The positions of nu(as)(COC) and CH(2) wagging and rocking modes indicate a helical arrangement of the OEG chains. The D-OEG SAMs were exposed to 25 muM Br(2) in two ways: (i) by immersion into the Br(2) solution and (ii) in the galvanic cell Au|D-OEG SAM|25 muM Br(2) + 0.1 M Na(2)SO(4)|| 50 muM KBr + 0.1 M Na(2)SO(4)|Au. In the galvanic cell, the oxidant (Br(2)) is scavenged by a heterogeneous electron transfer reaction, slowing the reaction of D-OEG with Br(2). The slow progress of the reaction with Br(2) allowed us to draw conclusions about molecular rearrangements taking place during this reaction. The reaction with Br(2) starts on boundaries and/or defects present in the SAM. First, at the defect place, the alpha-C atom of the OEG chain reacts with Br(2) and the OEG part of the molecule is removed from the monolayer. In consequence an increase in disorder in the OEG part of the SAM is observed. The same mechanism of the reaction with Br(2) is applied for the d-dodecane alkanethiol part of the molecule. This reaction is slow, thus the order and the tilt of the hydrocarbon chain changes only a little during the reaction time.

  18. Purification of radium-226 for the manufacturing of actinium-225 in a cyclotron for alpha-immunotherapy; Radium-Aufreinigung zur Herstellung von Actinium-225 am Zyklotron fuer die Alpha-Immuntherapie

    Energy Technology Data Exchange (ETDEWEB)

    Marx, Sebastian Markus

    2014-09-23

    The thesis describes the development of methods for the purification of Ra-226. The objective was to obtain the radionuclide in the quality that is needed to be used as starting material in the manufacturing process for Ac-225 via proton-irradiated Ra-226. The radionuclide has been gained efficiently out of huge excesses of impurities. The high purity of the obtained radium affords its use as staring material in a pharmaceutical manufacturing process.

  19. The Technological Enhancement of Normally Occurring Radioactive Materials in Red Mud due to the Production of Alumina

    Directory of Open Access Journals (Sweden)

    Maurice O. Miller

    2016-01-01

    Full Text Available This study investigates the level of technological enhancement of normally occurring radioactive materials (TENORM in the red mud waste due to the production of alumina in Jamaica. Technological enhancements factors (TEF were determined for the uranium, thorium, actinium series, their progenies, and the nonseries potassium-40 using gamma spectrometry. The study concluded that bauxite production technologically enhances the uranium progenies Th-234, Pb-214, Bi-214, and Pa-234 and the thorium-232 progenies Ac-228, Pb-212, and Bi-212 in red mud. The actinium series was technologically enhanced, but K-40 and the thorium daughter, Tl-208, were reduced. The spectrometric comparison of Tl-208 (at 510 keV was unexpected since its other photopeaks at 583 keV, 934 keV, and 968 keV were markedly different. An explanation for this anomaly is discussed. An explanation regarding the process of accumulation and fractionation of organically derived phosphate deposits and potassium-feldspar is offered to explain the spectrometric differences between the alumina product and its waste material, red mud.

  20. Production of actinium, thorium and radium isotopes from natural thorium irradiated with protons up to 141 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Ermolaev, S.V.; Zhuikov, B.L.; Kokhanyuk, V.M.; Matushko, V.L. [Russian Academy of Sciences, Moscow (Russian Federation). Inst. of Nuclear Research; Kalmykov, S.N. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Chemistry Dept.; Aliev, R.A. [Lomonosov Moscow State Univ., Moscow (Russian Federation). Skobeltsyn Inst. of Nuclear Physics; Tananaev, I.G.; Myasoedov, B.F. [Russian Academy of Sciences, Moscow (Russian Federation). A.N. Frumkin Inst. of Physical Chemistry and Electrochemistry

    2012-07-01

    Cross sections of {sup 225}Ac, {sup 227}Ac, {sup 227}Th and {sup 228}Th in thorium-232 targets irradiated with protons in the energy range 21-141 MeV have been measured. Based on these data, production yields of {sup 225}Ac and {sup 223}Ra in thick thorium targets have been calculated. It is possible to produce in proton energy range 60-140 MeV about 96 GBq (2.6 Ci) {sup 225}Ac per 10-d irradiation with 100 {mu}A proton beam current and 10-d decay, and much higher amount of {sup 223}Ra. The impurities of {sup 227}Ac and {sup 224}Ra are important and need to be assessed for further medical applications. (orig.)

  1. Renal uptake of bismuth-213 and its contribution to kidney radiation dose following administration of actinium-225-labeled antibody

    Energy Technology Data Exchange (ETDEWEB)

    Schwartz, J; O' Donoghue, J A; Humm, J L [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); Jaggi, J S [Bristol-Myers Squibb, Plainsboro, NJ (United States); Ruan, S; Larson, S M [Nuclear Medicine Service Department of Radiology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065 (United States); McDevitt, M; Scheinberg, D A, E-mail: schwarj1@mskcc.org [Molecular Pharmacology and Chemistry, Sloan-Kettering Institute, 1275 York Avenue, New York, NY 10065 (United States)

    2011-02-07

    Clinical therapeutic studies using {sup 225}Ac-labeled antibodies have begun. Of major concern is renal toxicity that may result from the three alpha-emitting progeny generated following the decay of {sup 225}Ac. The purpose of this study was to determine the amount of {sup 225}Ac and non-equilibrium progeny in the mouse kidney after the injection of {sup 225}Ac-huM195 antibody and examine the dosimetric consequences. Groups of mice were sacrificed at 24, 96 and 144 h after injection with {sup 225}Ac-huM195 antibody and kidneys excised. One kidney was used for gamma ray spectroscopic measurements by a high-purity germanium (HPGe) detector. The second kidney was used to generate frozen tissue sections which were examined by digital autoradiography (DAR). Two measurements were performed on each kidney specimen: (1) immediately post-resection and (2) after sufficient time for any non-equilibrium excess {sup 213}Bi to decay completely. Comparison of these measurements enabled estimation of the amount of excess {sup 213}Bi reaching the kidney ({gamma}-ray spectroscopy) and its sub-regional distribution (DAR). The average absorbed dose to whole kidney, determined by spectroscopy, was 0.77 (SD 0.21) Gy kBq{sup -1}, of which 0.46 (SD 0.16) Gy kBq{sup -1} (i.e. 60%) was due to non-equilibrium excess {sup 213}Bi. The relative contributions to renal cortex and medulla were determined by DAR. The estimated dose to the cortex from non-equilibrium excess {sup 213}Bi (0.31 (SD 0.11) Gy kBq{sup -1}) represented {approx}46% of the total. For the medulla the dose contribution from excess {sup 213}Bi (0.81 (SD 0.28) Gy kBq{sup -1}) was {approx}80% of the total. Based on these estimates, for human patients we project a kidney-absorbed dose of 0.28 Gy MBq{sup -1} following administration of {sup 225}Ac-huM195 with non-equilibrium excess {sup 213}Bi responsible for approximately 60% of the total. Methods to reduce renal accumulation of radioactive progeny appear to be necessary for the success of {sup 225}Ac radioimmunotherapy.

  2. Coordination chemistry of the sup 212 Pb/ sup 212 Bi nuclear transformation: Alpha-emitting radiopharmaceuticals

    Energy Technology Data Exchange (ETDEWEB)

    Parks, N.J.; Harris, W.R.; Keen, C.L.; Cooper, S.R.

    1992-07-01

    Subdivisions of this project are: (a) the synthesis of prototypical thiolate and dithiocarbamate based hexacoordinate complexes, (b) radiochemical engineering for generation of no-carrier-added lead and bismuth radioelements, (c) the first isolation of bismuth-binding proteins from in vivo studies with cyclotron produced {sup 205/206}Bi tracer, and (d) initial development of transport mechanisms for the intracellular radiobiological study of alpha emitting bismuth, and (e) the initiation of chemical equilibrium studies and biochemical pathways with cyclotron-produced, no-carrier-added, {sup 203}Pb (T{sub 1/2} = 51 hr).

  3. 7 CFR 980.212 - Import regulations; tomatoes.

    Science.gov (United States)

    2010-01-01

    ... the Federal-State Inspection Service, Food Safety and Quality Service, United States Department of.... 33 1 All other points Chief, Fresh Products Branch, Fruit and Vegetable Quality Division, Food Safety... number, the truck and trailer license number, the name of the vessel, or other identification of the...

  4. 10 CFR 1015.212 - Interest, penalties and administrative costs.

    Science.gov (United States)

    2010-01-01

    ... percentage by which the Consumer Price Index for the month of June of the calendar year preceding the adjustment exceeds the Consumer Price Index for the month of June of the calendar year in which the debt was determined or last adjusted. Increases to administrative debts shall be computed annually. DOE will use...

  5. Subtask 2.12 - Air Quality Assessment and Control

    Energy Technology Data Exchange (ETDEWEB)

    Laura Raymond

    2007-07-30

    Past particulate matter (PM) research projects conducted at the Energy & Environmental Research Center included data on PM size, morphology, and chemistry. The objective of this project was to improve automated analysis capabilities of the scanning electron microscope (SEM) equipped with an energy-dispersive spectrometer. The SEM is now able to perform particle-by-particle analysis on the desired number of particles and provide size, morphology, and chemistry information for each particle. A new x-ray and image analysis system was purchased and implemented for improvements to data acquisition and analysis. This new analysis system is equipped with a digital-pulse processor, allowing for the determination of pixel-by-pixel chemistry, which significantly enhances our ability to characterize PM and other materials. In addition, this system is personal computer-based, which allows programming of the SEM to perform the automated image analysis along with detailed chemical information. This permits the incorporation of particle classification algorithms within the same computer system as the analysis is conducted. Additionally, the new Spirit software can now integrate full SEM control with imaging, elemental identification, and electron backscatter diffraction (EBSD) operation. The EBSD system has also allowed for phase identification within the SEM. Reexamination of previous samples collected on a polycarbonate filter for ambient-air PM2.5 analysis has shown that crystalline identification of individual particles can be done without further sample preparation or modification of the sample and/or sampling substrate.

  6. Dicty_cDB: VHE212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available DN816506 |A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low ...26 |A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Act...hanism For Low Ph Activation, mRNA sequence. 52 2e-16 4 ...rensis cDNA, mRNA sequence. 117 2e-23 3 DN814237 |A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mec

  7. Dicty_cDB: SHF212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available wimonas jakobiformis mitochondrial DNA, complete genome.... 42 7e-04 6 CT301628 |CT301628.1 Sus scrofa genomic clone CH242-214A22, genomic survey sequence. 40 8e-04 2 AF295546 |AF295546.1 Mala

  8. 212 Emotional Intelligence and Self Efficacy as Correlates of Career ...

    African Journals Online (AJOL)

    FIRST LADY

    2011-01-18

    Jan 18, 2011 ... Emotional Intelligence, Self Efficacy; Career Commitment. Introduction. Career ... There is no doubt that the choice of career is very crucial in the life of an individual. ..... Personality Development Needs and Counsellors Role.

  9. Mining (except Oil and Gas) Sector (NAICS 212)

    Science.gov (United States)

    EPA Regulatory and enforcement information for the mining sector, including metal mining & nonmetallic mineral mining and quarrying. Includes information about asbestos, coal mining, mountaintop mining, Clean Water Act section 404, and abandoned mine lands

  10. 29 CFR 1910.212 - General requirements for all machines.

    Science.gov (United States)

    2010-07-01

    ...) Alligator shears. (d) Power presses. (e) Milling machines. (f) Power saws. (g) Jointers. (h) Portable power... blades. When the periphery of the blades of a fan is less than seven (7) feet above the floor or...

  11. 212ʻ Fahrenheiti : eesti luule 2006 / Piret Bristol

    Index Scriptorium Estoniae

    Bristol, Piret, 1968-

    2008-01-01

    Järgmistest 2006. a. ilmunud luulekogudest: Ryytle, Indrek. Inglid rokijaamas. Puhja : I. Rüütle ; Runnel, Hando. Viru veri ei värise. Tartu : Ilmamaa ; Vabat, Martin. Mina olengi kirjandusklassik. Tartu : Eesti Kirjanduse Selts ; Korts, Eliina. Lööklaused murravad metsi. Tartu : Eesti Kirjanduse Selts ; Koff, Indrek. Vana laul. Luige : Verb ; Teede, Andra. Takso Tallinna taevas. Luige : Verb ; Vusser, Kaspar. Vusserduste võnked. Tallinn : P. Kukk ; Elbing, Andrus. Siin Beebilõust, tere! : häired pimelinna tänavalt = Ciao, it's Babyface : trouble from the streets of a blind city. Tallinn : Epifanio ; Hint, Miina. Vabaduse vang, ehk, Põrandaalune kirjandus. Tallinn : MR ; Jüriado, Tiiu. Luule on ime. Habaja : Kentaur ; Ligi, Katre. Naabrivalve. Tartu : Ilmamaa ; Krull, Hasso. Talv. Tallinn : Tuum ; Mets, Mae. Pühapäeval on reede. Tartu : Ilmamaa ; Ploom, Ülar. Porr ja sorry. Tallinn : Tuum ; Hirv, Indrek, Surmapõletaja. Tallinn : Tuum ; Piir, Milvi Martina. Kõrkjavaas. Tartu : Fantaasia ; Soomets, Triin. Väljas. Tallinn : Tuum ; Mathura. Kohalolu. Rapla : Allikäärne ; Afanasjev, Vahur. Katedraal Emajões. Tartu ; Brüssel : ID Salong//Sild, Ivar. Spermaga ja puha. Pärnu : Jumalikud Ilmutused ; Meiel, Kaupo. Polügrafisti käsiraamat. Pärnu : Jumalikud Ilmutused ; Kivisildnik, Sven. Vägistatud jäämägi. Pärnu : Jumalikud Ilmutused ; Arder, Ott. Luule sünnib kus sünnib kui sünnib / koost. Leelo Tungal. Tallinn : Tänapäev ; Kompus, Marko. Vallaliste jõgede tõkkejooksja. Tartu : M. Kompus

  12. 2-[1-(2-Hydroxy-4-methoxyphenylethylidene]-N-methylhydrazinecarbothioamide

    Directory of Open Access Journals (Sweden)

    Jerry P. Jasinski

    2013-08-01

    Full Text Available In the title compound, C11H15N3O2S, the dihedral angle between the mean planes of the benzene ring and hydrazinecarbothioamide group is 9.2 (1°. An intramolecular O—H...N hydrogen bond is observed, serving to maintain an approximately planar conformation for the molecule. In the crystal, inversion dimers linked by C—H...O interactions occur. Further C—H...O contacts link dimers into (010 chains.

  13. 7 CFR 62.212 - Official assessment reports.

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS INSPECTION ACT (CONTINUED) LIVESTOCK, MEAT, AND OTHER AGRICULTURAL COMMODITIES (QUALITY SYSTEMS VERIFICATION PROGRAMS) Quality Systems Verification...

  14. 8 CFR 212.1 - Documentary requirements for nonimmigrants.

    Science.gov (United States)

    2010-01-01

    ... identified by the Department of State as a source country of refugees designated of special humanitarian... gives rise to any cause of action or claim under this paragraph or any other law against any official of... basis for good cause including, but not limited to if: The admissions of visitors from a country have...

  15. 33 CFR 110.212 - Newport Bay Harbor, Calif.

    Science.gov (United States)

    2010-07-01

    ... at the east end of Lido Isle; north of a line parallel to and 250 feet north of a line bearing 268° from Newport Bay Channel Light 11, this line being the north line of the main fairway; northwest of a... parallel to the existing pierhead line, and adjoining and on the north side of Anchorage C-3 described...

  16. Dicty_cDB: AFG212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ielielpeyvlsllfgsvmfvfififiiiayrfkeidqsvldelngdt pmtddnananskeldvvtqkdlssssnpiinn...nnnnnnstlgns Frame C: kkennyyykkwr*kknhqilvyqhqlii*ghqlmervhlkvnkviwkvlimkflfmegmk iifhhqlnilwvmkfvkgihtml*e

  17. 14 CFR 221.212 - Special tariff permission.

    Science.gov (United States)

    2010-01-01

    ... in the Government Filing File and the Filing Advice Status File, and shall be referenced in such a... in the Government Filing File and the Filing Advice Status File. (d) When the paper portion of a...) When a filer submits an electronic tariff or an amendment to an electronic tariff for which authority...

  18. 49 CFR 212.207 - Signal and train control inspector.

    Science.gov (United States)

    2010-10-01

    ... Signal Systems (49 CFR part 236), to make reports of those inspections, and to recommend the institution... knowledge of signal and train control systems, maintenance practices, test and inspection techniques; (2... standards accepted in the industry; and (ii) The Rules, Standards and Instructions for Railroad...

  19. 7 CFR 868.212 - Special grades and requirements.

    Science.gov (United States)

    2010-01-01

    .... Tolerances for live insects for infested rough rice are defined according to sampling designations as follows...) of this section will be considered infested if the representative sample contains two or more live weevils, or one live weevil and one or more other live insects injurious to stored rice or five or...

  20. Dicty_cDB: SFB212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available se isozyme 3 mRNA, complete cds. 58 2e-07 2 CK273593 |CK273593.1 EST719671 potato abiotic stress cDNA librar...34 An expressed sequence tag database for abiotic stressed leaves of Vitis vinife...066D06_332552 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonn...19214.1 VVB158G03_410335 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera va

  1. 48 CFR 14.212 - Economic purchase quantities (supplies).

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Economic purchase... purchase quantities (supplies). Contracting officers shall comply with the economic purchase quantity... provision at 52.207-4, Economic Purchase Quantity—Supplies, and 7.204 for guidance on handling responses...

  2. 212ʻ Fahrenheiti : eesti luule 2006 / Piret Bristol

    Index Scriptorium Estoniae

    Bristol, Piret, 1968-

    2008-01-01

    Järgmistest 2006. a. ilmunud luulekogudest: Ryytle, Indrek. Inglid rokijaamas. Puhja : I. Rüütle ; Runnel, Hando. Viru veri ei värise. Tartu : Ilmamaa ; Vabat, Martin. Mina olengi kirjandusklassik. Tartu : Eesti Kirjanduse Selts ; Korts, Eliina. Lööklaused murravad metsi. Tartu : Eesti Kirjanduse Selts ; Koff, Indrek. Vana laul. Luige : Verb ; Teede, Andra. Takso Tallinna taevas. Luige : Verb ; Vusser, Kaspar. Vusserduste võnked. Tallinn : P. Kukk ; Elbing, Andrus. Siin Beebilõust, tere! : häired pimelinna tänavalt = Ciao, it's Babyface : trouble from the streets of a blind city. Tallinn : Epifanio ; Hint, Miina. Vabaduse vang, ehk, Põrandaalune kirjandus. Tallinn : MR ; Jüriado, Tiiu. Luule on ime. Habaja : Kentaur ; Ligi, Katre. Naabrivalve. Tartu : Ilmamaa ; Krull, Hasso. Talv. Tallinn : Tuum ; Mets, Mae. Pühapäeval on reede. Tartu : Ilmamaa ; Ploom, Ülar. Porr ja sorry. Tallinn : Tuum ; Hirv, Indrek, Surmapõletaja. Tallinn : Tuum ; Piir, Milvi Martina. Kõrkjavaas. Tartu : Fantaasia ; Soomets, Triin. Väljas. Tallinn : Tuum ; Mathura. Kohalolu. Rapla : Allikäärne ; Afanasjev, Vahur. Katedraal Emajões. Tartu ; Brüssel : ID Salong//Sild, Ivar. Spermaga ja puha. Pärnu : Jumalikud Ilmutused ; Meiel, Kaupo. Polügrafisti käsiraamat. Pärnu : Jumalikud Ilmutused ; Kivisildnik, Sven. Vägistatud jäämägi. Pärnu : Jumalikud Ilmutused ; Arder, Ott. Luule sünnib kus sünnib kui sünnib / koost. Leelo Tungal. Tallinn : Tänapäev ; Kompus, Marko. Vallaliste jõgede tõkkejooksja. Tartu : M. Kompus

  3. 14 CFR 212.9 - Prior authorization requirements.

    Science.gov (United States)

    2010-01-01

    ... long-term wet lease to a foreign air carrier. (b) Foreign air carriers shall obtain a statement of...; (2) Long-term wet lease; (3) Charter flight for which the Department specifically requires prior.... air carrier guaranteed by a bilateral agreement; or (3) Has otherwise impaired, limited, or denied...

  4. 14 CFR 212.10 - Application for statement of authorization.

    Science.gov (United States)

    2010-01-01

    ... lease agreement. Except for an application for a long-term wet lease involving a codeshare agreement, an... long-term wet lease involving a codeshare agreement shall be submitted to DOT Dockets, PL-401, 1200 New... submitted on OST Form 4540 except that for part charters or long-term wet leases the application may be...

  5. 40 CFR 156.212 - Personal protective equipment statements.

    Science.gov (United States)

    2010-07-01

    ...: (body protection statement); (glove statement, if applicable); (footwear statement, if applicable... shall be based on the more toxic (lower numbered) category. 2 For labeling language for chemical-resistant gloves, see paragraph (f) of this section. 3 For labeling language for respiratory...

  6. 212 The Functionality of Being in Pantaleon's Operative ...

    African Journals Online (AJOL)

    User

    2012-01-24

    Jan 24, 2012 ... concept of being as understood by Pantaleon Iroegbu, as being qua ... The emergence of the Medieval Epoch did not alter the centrepiece of ... These notwithstanding, in recent times, African thinkers have tried to redefine ...

  7. 36 CFR 212.5 - Road system management.

    Science.gov (United States)

    2010-07-01

    ...) The load, weight, length, height, and width limitations of vehicles shall be in accordance with the... officials should give priority to decommissioning those unneeded roads that pose the greatest risk to public...: (i) Deposits received for work on adjacent and overlapping areas may be combined when it is the...

  8. Session 21.2 - Measurement of Light at Night

    Science.gov (United States)

    Wainscoat, Richard J.

    2016-10-01

    The introduction of the mercury vapor lamp for general lighting in the 1930s probably marked the beginning of significant light pollution. Lighting levels have increased slowly, year-to-year, with sky brightness levels increasing only slowly on timescales of a year; no measurement protocols or instruments existed to quantify this increase. However, on timescales of 10-20 years, or on generational timescales, the increases in night sky levels, particularly in urban areas, have been dramatic. Younger people speak with their parents or grandparents who remark how beautiful the sky used to be, and how many stars they could see when they when they were younger. Older people can themselves remember how many stars were visible in the sky when they were younger. Whole generations of children now grow up without ever seeing the Milky Way. Society has not had tools to easily measure sky brightness, and monitoring from space has only recently become available. A subtle increase of 10% sky brightness per year, for example, is not noticeable to the human eye on the time scale of a year, and has been tolerated by society. But such an increase compounds to an increase of a factor 2.6 in 10 years, 6.7 in 20 years, and a factor 45 in 40 years, corresponding to a dramatic increase in sky brightness, an almost complete loss in ability to see faint objects in the night sky, and rendering the sky unusable for most forms of astronomy. The most striking examples are the urban observatories found in many major cities that can no longer be used. Session 2 was primarily focused on measurement of light at night, with an emphasis on measurement of light pollution. It comprised of 6 papers that are summarized below. Over the last decade, our ability to measure light pollution has grown tremendously, and the instrumentation needed to produce reliable quantitative measurements has become much more affordable, and now includes consumer grade digital cameras and even smart phones. During this same time period, light pollution has continued to grow. The widespread changes from mostly high-pressure sodium lighting to LED lighting that are now occurring make continued monitoring and measurement of light pollution particularly important into the future. Complete presentations may be viewed at: http://www.noao.edu/education/IAUGA2015FM21

  9. Dicty_cDB: SHJ212 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available s witches'-broom phy... 34 3.7 AY754825_1( AY754825 |pid:none) Human rotavirus C isolate DhakaC13... 33 4.8 ...... 33 8.2 FJ619589_1( FJ619589 |pid:none) Human rotavirus C strain 9298 VP4 ...

  10. 8 CFR 212.6 - Border crossing identification cards.

    Science.gov (United States)

    2010-01-01

    ... after October 1, 2002. If presented on or after that date, these documents will be voided at the POE. (d) Voidance for reasons other than expiration of the validity of the form—(1) At a POE. (i) In accordance with... by a supervisory immigration officer at a POE if it is considered void pursuant to section 222(g)...

  11. 2-{1-[(2-Nitrobenzenesulfonamidomethyl]cyclohexyl}acetic acid

    Directory of Open Access Journals (Sweden)

    Nosheen Kanwal

    2012-01-01

    Full Text Available In the title compound, C15H20N2O6S, the C—SO2—NH—C torsion angle is 64.54 (14°. In the molecule, there is a bifurcated N—H...(O,O hydrogen bond, forming S(7 rings. In the crystal, inversion dimers are formed via O—H...O hydrogen bonds involving the carboxyl group, so forming R22(8 rings. These dimers are further linked via pairs of C—H...O hydrogen bonds, forming a C(6 chain propagating along the c-axis direction.

  12. Coordination chemistry of the {sup 212}Pb/{sup 212}Bi nuclear transformation: Alpha-emitting radiopharmaceuticals. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    Parks, N.J.; Harris, W.R.; Keen, C.L.; Cooper, S.R.

    1992-07-01

    Subdivisions of this project are: (a) the synthesis of prototypical thiolate and dithiocarbamate based hexacoordinate complexes, (b) radiochemical engineering for generation of no-carrier-added lead and bismuth radioelements, (c) the first isolation of bismuth-binding proteins from in vivo studies with cyclotron produced {sup 205/206}Bi tracer, and (d) initial development of transport mechanisms for the intracellular radiobiological study of alpha emitting bismuth, and (e) the initiation of chemical equilibrium studies and biochemical pathways with cyclotron-produced, no-carrier-added, {sup 203}Pb (T{sub 1/2} = 51 hr).

  13. 8 CFR 1212.4 - Applications for the exercise of discretion under section 212(d)(1) and 212(d)(3).

    Science.gov (United States)

    2010-01-01

    ... IMMIGRATION REVIEW, DEPARTMENT OF JUSTICE IMMIGRATION REGULATIONS DOCUMENTARY REQUIREMENTS: NONIMMIGRANTS... statement of the history of the applicant's membership or affiliation, including the period of...

  14. Central Asia Since 1991: The Experience of the New Independent States. OECD Development Centre Working Paper, No. 212 (Formerly Technical Paper, No. 212)

    Science.gov (United States)

    Pomfret, Richard

    2003-01-01

    The five former Soviet republics of Central Asia (Uzbekistan, Kazakhstan, the Kyrgyz Republic, Tajikistan, and Turkmenistan) have become separate states, developing at different rates and in different directions, and with different political and economic regimes. As a result, the cohesion of the region has broken down and economic development is…

  15. Soil and river sediments radionuclides monitoring at Aramar Experimental Center: an historical overview

    Energy Technology Data Exchange (ETDEWEB)

    Segre, Nadia; Fagundes, Rosane Correa, E-mail: nadia.segre@ctmsp.mar.mil.br [Centro Tecnologico da Marinha (CTM-SP/CEA/LARE), Ipero, SP (Brazil). Centro Experimental Aramar. Lab. Radioecologico; Moraes, Marco Antonio P.V. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    In order to evaluate possible effects to the environment resulting from the implementation of the Centro Tecnologico da Marinha - Centro Experimental Aramar (CTMSP-CEA) at Ipero in Sao Paulo state, Brazil, which came into operation in 1989, an Environmental Monitoring Program (PMA) was established in October, 1987. One of the aims of this program is to monitor the soil and river sediments radionuclides levels at CEA and beyond its boundary. The utilization of statistical tools to evaluate the results of radiometric environmental monitoring is a procedure required by National Nuclear Energy Commission (CNEN). The box plot is a simple statistical tool for displaying data. The central tendency and dispersion of the results as well as the observation of unusual results (outliers) in the dataset are easily visualized. Control chart is a graph that maps data and provides a picture of how a process is performing over time. A control chart always has a central line for the mean, an upper line for the upper control limit and a lower line for the lower control limit. Box plots and control charts were used to visualize the annual amount of natural uranium, lead-214, actinium-228 and lead-212 in soil and river sediment detected between 1987 and 2011, considering the measurements of all monitored places each year. This historical observation shows that, in average, the results obtained are below than the 1987-1988 levels (CEA's pre-operational) or below than the backgrounds radionuclides values. (author)

  16. Evaluation of naturally occurring radioactive materials (NORMs) in inorganic and organic oilfield scales from the Middle East.

    Science.gov (United States)

    Bassioni, Ghada; Abdulla, Fareed; Morsy, Zeinab; El-Faramawy, Nabil

    2012-04-01

    The distribution of natural nuclide gamma-ray activities and their respective annual effective dose rates, produced by potassium-40 (⁴⁰K), uranium-238 (²³⁸U), thorium-232 (²³²Th), and radium-226 (²²⁶Ra), were determined for 14 oilfield scale samples from the Middle East. Accumulated radioactive materials concentrate in tubing and surface equipment, and workers at equipment-cleaning facilities and naturally occurring radioactive materials (NORMs) disposal facilities are the population most at risk for exposure to NORM radiation. Gamma-spectra analysis indicated that photo-gamma lines represent the parents of 10 radioactive nuclides: ²³⁴Th, plutonium-239, actinium-228, ²²⁶Ra, lead-212 (²¹²Pb), ²¹⁴Pb, thallium-238 (²⁰⁸Tl), bismuth-212 (²¹²Bi), ²¹⁴Bi, and ⁴⁰K. These nuclides represent the daughters of the natural radioactive series ²³⁸U and ²³²Th with ⁴⁰K as well. The mean activity concentration of ²³⁸U, ²³²Th, and ⁴⁰K were found to be 25.8 ± 11.6, 18.3 ± 8.1, and 4487.2 ± 2.5% Bq kg⁻¹ (average values for 14 samples), respectively. The annual effective dose rates and the absorbed doses in air, both indoor and outdoor, for the samples were obtained as well. The results can be used to assess the respective hazard on workers in the field and represent a basis for revisiting current engineering practices.

  17. Environmental Assessment: Demolish Buildings 212, 218, 819, 820 at Grand Forks Air Force Base

    Science.gov (United States)

    2007-01-31

    View Nature Preserve” has been developed to restore a part of the native tallgrass prairie that once was dominant in this region. Plants thriving in...and many native wildflower species. The Grand Forks AFB Natural Resources Manager and volunteers installed a butterfly garden in the Prairie View...characterized as typical prairie potholes found within the northern plains ecoregion. Wetlands on Grand Forks AFB occur frequently in drainage

  18. 48 CFR 52.212-3 - Offeror Representations and Certifications-Commercial Items.

    Science.gov (United States)

    2010-10-01

    ..., mineral extraction activities, oil-related activities, or the production of military equipment, as those...). _Asian-Pacific American (persons with origins from Burma, Thailand, Malaysia, Indonesia,...

  19. 8 CFR 212.15 - Certificates for foreign health care workers.

    Science.gov (United States)

    2010-01-01

    ... scores on the English tests administered by ETS: Test Of English as a Foreign Language (TOEFL): Paper...: (A) ETS: TOEFL: Paper-Based 540, Computer-Based 207; TWE: 4.0; TSE: 50; (B) TOEIC Service... certificate: (A) ETS: TOEFL: Paper-Based 530, Computer-Based 197; TWE: 4.0; TSE: 50; (B) TOEIC...

  20. 36 CFR 212.7 - Access procurement by the United States.

    Science.gov (United States)

    2010-07-01

    ..., limitations and other provisions set forth in the easement, permit, or other indenture. This control by the United States may include restricting or conditioning the use of the interest owned by the United...

  1. Hyperspectral Imagery for the Main Eight Hawaiian Islands:Oahu (212-0612-332211)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort among the National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment; the...

  2. Hyperspectral Imagery for the Main Eight Hawaiian Islands:Oahu (212-0612-272217)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort among the National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment; the...

  3. Steady State Comparisons HAWC2 v12.2 vs HAWCStab2 v2.12

    DEFF Research Database (Denmark)

    Verelst, David Robert; Hansen, Morten Hartvig; Pirrung, Georg

    This reports presents comparison of the steady state HAWC2 [1] [2] [3] simulation results and the HAWCStab2 computations of the DTU10MW reference turbine [4] [5]. It serves as a simple validation for the HAWCStab2 [6] [7] [8] steady state computations....

  4. 10 CFR 72.212 - Conditions of general license issued under § 72.210.

    Science.gov (United States)

    2010-01-01

    ..., considering potential amplification of earthquakes through soil-structure interaction, and soil liquefaction potential or other soil instability due to vibratory ground motion; and (C) the requirements of §...

  5. 76 FR 18865 - Airworthiness Directives; Bell Helicopter Textron, Inc. Model 212 Helicopters

    Science.gov (United States)

    2011-04-06

    ... Docket Management Facility between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays. The... refinish the fitting using a vibrating stylus (not to exceed 0.005 inch depth nor to extend within...

  6. 48 CFR 52.212-4 - Contract Terms and Conditions-Commercial Items.

    Science.gov (United States)

    2010-10-01

    ... with the cost accounting standards or contract cost principles for this purpose. This paragraph does... software; (5) solicitation provisions if this is a solicitation; (6) other paragraphs of this clause; (7... be required to comply with the cost accounting standards or contract cost principles for this...

  7. CAVEMAN: Configuration And VErsion MANagement. User`s guide, v2.1.2

    Energy Technology Data Exchange (ETDEWEB)

    Dyer, K.M.

    1993-03-12

    The mission of the CAVEMAN project is to provide the tools for large code systems so that those who work on them can perform source control and configuration control on UNIX-based file servers, rather than the LC Crays. An outside product called cvs has been chosen as the underlying source control system. The initials stand for {open_quotes}Concurrent Versions System{close_quotes}. It is a freeware program that runs on most UNIX stations. To quote from the cvs man page: {open_quotes}cvs is a front end to the rcs revision control system which extends the notion of revision control from a collection of files in a single directory to a hierarchical collection of directories consisting of revision controlled files. These directories and files can be combined together to form a software release. cvs provides the functions necessary to manage these software releases and to control the concurrent editing of source files among multiple software developers.{close_quotes} While cvs may be run directly, there are several tasks in the LLNL environment that it seemed either safer or easier to automate. CAVEMAN provides the needed functionality, generally in the form of wrappers to cvs.

  8. Integrated radwaste treatment system lessons learned from 2{1/2} years of operation

    Energy Technology Data Exchange (ETDEWEB)

    Baker, M.N.; Fussner, R.J.

    1997-05-01

    The Integrated Radwaste Treatment System (IRTS) at the West Valley Demonstration Project (WVDP) is a pretreatment scheme to reduce the amount of salts in the high-level radioactive waste (vitrification) stream. Following removal of cesium-137 (Cs-137) by ion-exchange in the Supernatant Treatment System (STS), the radioactive waste liquid is volume-reduced by evaporation. Trace amounts of Cs-137 in the resulting distillate are removed by ion-exchange, then the distillate is discharged to the existing plant water treatment system. The concentrated product, 37 to 41 percent solids by weight, is encapsulated in cement producing a stable, low-level waste form. The Integrated Radwaste Treatment System (IRTS) operated in this mode from May 1988 through November 1990, decontaminating 450,000 gallons of high-level waste liquid; evaporating and encapsulating the resulting concentrates into 10,393 71-gallon square drums. A number of process changes and variations from the original operating plan were required to increase the system flow rate and minimize waste volumes. This report provides a summary of work performed to operate the IRTS, including system descriptions, process highlights, and lessons learned.

  9. 75 FR 81433 - Airworthiness Directives; Airbus Model A321-211, -212, -231, and -232 Airplanes

    Science.gov (United States)

    2010-12-28

    ... necessary. The corrective actions include performing a manual pull test to confirm the integrity of the ring... Bulletin A320-28A1173, dated October 21, 2008. The actions described in this service information are... Instructions of Airbus Service Bulletin A320-28A1173, dated October 21, 2008. FAA AD Differences Note 1:...

  10. 14 CFR 212.5 - Operation of affinity (pro rata) charters.

    Science.gov (United States)

    2010-01-01

    ... who acts not as a principal, but as an agent for the chartering organization or the certificated or... members of an eligible chartering organization, and their immediate families (spouse, children, and... members of the chartering organization for a minimum of six months prior to the date of commencement of...

  11. 75 FR 5681 - Airworthiness Directives; Bell Helicopter Textron, Inc. Model 205B and 212 Helicopters

    Science.gov (United States)

    2010-02-04

    ..., Rotorcraft Directorate, Rotorcraft Certification Office, 2601 Meacham Blvd., Fort Worth, Texas 76137... condition is likely to exist or develop on other helicopters of the same type design. Therefore, this AD is... 39.19. Contact the Manager, Rotorcraft Certification Office, ATTN: Michael Kohner, Aviation...

  12. 23 CFR 972.212 - Federal lands safety management system (SMS).

    Science.gov (United States)

    2010-04-01

    ... transportation facilities serving the Refuge System, as appropriate, funded under the FLHP. (b) The FWS SMS may....), location, description of event, severity, weather and cause; (ii) An inventory of safety appurtenances such... transportation facilities serving the Refuge System funded under the FLHP, the extent of system requirements (e.g...

  13. 2016 Presidential Address: Diabetes at 212°-Confronting the Invisible Disease.

    Science.gov (United States)

    Schatz, Desmond

    2016-10-01

    This address was delivered by Desmond Schatz, MD, President, Medicine & Science, of the American Diabetes Association (ADA), at the Association's 76th Scientific Sessions in New Orleans, LA, on 12 June 2016. Dr. Schatz is Professor and Associate Chairman of Pediatrics, Medical Director of the University of Florida Diabetes Institute, and Director of the Clinical Research Center at the University of Florida, Gainesville, FL. A physician-scientist, he has been involved in type 1 diabetes research since the mid-1980s and has published over 300 articles and book chapters. Dr. Schatz's research focuses on the prediction, natural history, genetics, immunopathogenesis, and prevention of type 1 diabetes, as well as the treatment of children and adolescents with type 1 diabetes. He is the principal investigator on several National Institutes of Health, JDRF, and other competitively funded grants. Dr. Schatz earned his medical degree from the University of the Witwatersrand School of Medicine in Johannesburg, South Africa, and completed his residency and fellowship at the University of Florida. He has been an ADA volunteer for over 30 years and has served on the Professional Practice Committee (twice), Scientific Sessions Meeting Planning Committee, Publications Policy Committee (Chair), Government Relations Committee, Youth Strategies Committee, and Board of Directors. A board-certified pediatric endocrinologist, Dr. Schatz is the 2016 recipient of the Banting Medal for Leadership and Service from the ADA for his outstanding commitment and contributions to the Association.

  14. H 2-12, A Misidentified Planetary Nebula in Kepler SNR

    Directory of Open Access Journals (Sweden)

    H. Riesgo

    2005-01-01

    Full Text Available Presentamos imágenes de banda angosta y espectros echelle de rendija larga de H 2–12. Esta nebulosa ha sido clasificada como una nebulosa planetaria. Sin embargo, los datos presentados aquí muestran que este objeto es en realidad parte de los nudos y filamentos más brillantes localizados en la parte occidental de la remanente de supernova de Kepler. Por lo tanto, la clasificación de H 2–12 como una nebulosa planetaria debe ser considerada como errónea.

  15. Case 212: chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids.

    Science.gov (United States)

    Bag, Asim K; Davenport, Jake J; Hackney, James R; Roy, Rasmoni; Fathallah-Shaykh, Hassan M

    2014-12-01

    History A previously healthy 23-year-old white man presented to the emergency department of our hospital with a 2-month history of dysarthria, progressively worsening vertigo, and difficulty walking. A diagnosis of retinitis pigementosa was made in this patient's childhood. He did not have any history of congenital syphilis. He did not have a history of nausea or vomiting, fever, weight loss, headache, photophobia, seizure, extremity weakness, or sensory disturbance. Physical examination revealed dysarthria, dysmetria, and ataxia. Kernig and Brudzinski signs were absent, and pathergy test results were negative. Laboratory evaluation revealed normal complete and differential blood counts and normal serum chemistry, including a normal serum angiotensin-converting enzyme level. Analysis of his serum was negative for antinuclear antibody (or ANA), cytoplasmic antineutrophil cvtoplasmic antibody (or cANCA), Sjögren syndrome antigens A and B (SS-A and SS-B, respectively), antitissue transglutaminase and antiendomysial antibodies, and paraneoplastic profile. Serum analysis was also negative for human immunodeficiency virus type 1 and type 2 RNA, Venereal Disease Research Laboratory (VDRL) test, rapid plasma regain (RPR), and fluorescent treponemal antibody absorption. Cerebrospinal fluid (CSF) analysis revealed clear fluid, a normal glucose level (64 mg/dL [3.6 mmol/L]; normal range, 40-70 mg/dL [2.2-3.9 mmol/L]), an elevated protein level (97 mg/dL; normal range, 12-60 mg/dL), and an elevated white blood cell count (7/mm(3) [0.007 ×10(9)/L] in tube 1 and 17/mm(3) [0.017 × 10(9)/L] in tube 2) with 84% lymphocytes. CSF immunoglobulin G level was elevated (30.1 mg/dL; normal, <5.9 mg/dL); however, there were no oligoclonal bands. Gram staining, acid-fast staining, and lactic acid, cryptococcal antigen, histoplasma antigen, herpes simplex virus polymerase chain reaction, VDRL, and RPR test results for CSF were negative. CSF did not grow any bacteria, fungus, or acid-fast bacillus at culture. CSF flow cytometry did not reveal a monoclonal lymphoid population. Initial imaging included brain magnetic resonance (MR) imaging. Computed tomography (CT) images of the chest, abdomen, and pelvis were normal (not shown). The patient's clinical symptoms and imaging findings responded to treatment with a high dose of oral steroids. However, the patient's symptoms exhibited clinical and radiologic progression after several attempts to taper the steroid dose.

  16. 212 Wealth Creation and Poverty Alleviation in Nigeria: The Role of ...

    African Journals Online (AJOL)

    User

    2010-10-17

    Oct 17, 2010 ... Indexed African Journals Online: www.ajol.info. An International ... College of Education, Nsugbe, Anambra State, Nigeria. GSM: + ... which they can only break with effective platform for using sophisticated technology to ...

  17. 41 CFR 101-6.212-4 - Procedures, evidence, and record.

    Science.gov (United States)

    2010-07-01

    .... (a) The hearing, decision, and any administrative review thereof shall be conducted in conformity... examination by the parties and opportunity shall be given to refute facts and arguments advanced on...

  18. Angiotensin II Regulates microRNA-132/-212 in Hypertensive Rats and Humans

    DEFF Research Database (Denmark)

    Eskildsen, Tilde V; Jeppesen, Pia L; Schneider, Mikael;

    2013-01-01

    MicroRNAs (miRNAs), a group of small non-coding RNAs that fine tune translation of multiple target mRNAs, are emerging as key regulators in cardiovascular development and disease. MiRNAs are involved in cardiac hypertrophy, heart failure and remodeling following cardiac infarction; however, mi...

  19. Resolución no. 212/05 : Recepción de estudiantes extranjeros

    OpenAIRE

    2005-01-01

    La Dirección de Relaciones Institucionales solicita se arbitren los medios para la recepción de estudiantes extranjeros en el marco de la Ley 25.871 de Política Migratoria Argentina, por lo que se aprueba un mecanismo para sistematizar la recepción de los estudiantes que se explicita en la resolución.

  20. 49 CFR 212.213 - Motive power and equipment (MP&E) inspector.

    Science.gov (United States)

    2010-10-01

    ...), Safety Glazing Standards (49 CFR part 223), Locomotive Safety Standards (49 CFR part 229), Safety Appliance Standards (49 CFR part 231), and Power Brake Standards (49 CFR part 232), to make reports of those... Standards, Locomotive Safety Standards, Safety Appliance Standards and Power Brake Standards. (3)...

  1. SU-E-J-212: Identifying Bones From MRI: A Dictionary Learnign and Sparse Regression Approach

    Energy Technology Data Exchange (ETDEWEB)

    Ruan, D; Yang, Y; Cao, M; Hu, P; Low, D [UCLA, Los Angeles, CA (United States)

    2014-06-01

    Purpose: To develop an efficient and robust scheme to identify bony anatomy based on MRI-only simulation images. Methods: MRI offers important soft tissue contrast and functional information, yet its lack of correlation to electron-density has placed it as an auxiliary modality to CT in radiotherapy simulation and adaptation. An effective scheme to identify bony anatomy is an important first step towards MR-only simulation/treatment paradigm and would satisfy most practical purposes. We utilize a UTE acquisition sequence to achieve visibility of the bone. By contrast to manual + bulk or registration-to identify bones, we propose a novel learning-based approach for improved robustness to MR artefacts and environmental changes. Specifically, local information is encoded with MR image patch, and the corresponding label is extracted (during training) from simulation CT aligned to the UTE. Within each class (bone vs. nonbone), an overcomplete dictionary is learned so that typical patches within the proper class can be represented as a sparse combination of the dictionary entries. For testing, an acquired UTE-MRI is divided to patches using a sliding scheme, where each patch is sparsely regressed against both bone and nonbone dictionaries, and subsequently claimed to be associated with the class with the smaller residual. Results: The proposed method has been applied to the pilot site of brain imaging and it has showed general good performance, with dice similarity coefficient of greater than 0.9 in a crossvalidation study using 4 datasets. Importantly, it is robust towards consistent foreign objects (e.g., headset) and the artefacts relates to Gibbs and field heterogeneity. Conclusion: A learning perspective has been developed for inferring bone structures based on UTE MRI. The imaging setting is subject to minimal motion effects and the post-processing is efficient. The improved efficiency and robustness enables a first translation to MR-only routine. The scheme generalizes to multiple tissue classes.

  2. Tank 241-AX-103, cores 212 and 214 analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    Steen, F.H.

    1998-02-05

    This document is the analytical laboratory report for tank 241-AX-103 push mode core segments collected between July 30, 1997 and August 11, 1997. The segments were subsampled and analyzed in accordance with the Tank 241-AX-103 Push Mode Core Sampling and Analysis Plan (TSAP) (Comer, 1997), the Safety Screening Data Quality Objective (DQO) (Dukelow, et al., 1995) and the Data Quality Objective to Support Resolution of the Organic Complexant Safety Issue (Organic DQO) (Turner, et al., 1995). The analytical results are included in the data summary table (Table 1). None of the samples submitted for Differential Scanning Calorimetry (DSC), Total Alpha Activity (AT), plutonium 239 (Pu239), and Total Organic Carbon (TOC) exceeded notification limits as stated in the TSAP (Conner, 1997). The statistical results of the 95% confidence interval on the mean calculations are provided by the Tank Waste Remediation Systems Technical Basis Group in accordance with the Memorandum of Understanding (Schreiber, 1997) and not considered in this report.

  3. N-Ethyl-2-[1-(2-hydroxy-4-methylphenylethylidene]hydrazinecarbothioamide

    Directory of Open Access Journals (Sweden)

    Brian J. Anderson

    2014-06-01

    Full Text Available The title compound, C12H17N3OS, crystallizes with two independent molecules (A and B in the asymmetric unit. The dihedral angle between the mean planes of the benzene ring and the hydrazinecarbothioamide group are 6.9 (4 and 37.2 (5° in molecules A and B, respectively. An intramolecular O—H...N hydrogen bond is observed in each molecule. This serves to maintain an approximately planar conformation for molecule A, but leaves a significant twist between these two groups in molecule B. In the crystal, a weak N—H...S interaction is observed, forming inversion dimers among the B molecules and resulting in an R22(8 motif. These dimers are further interconnected by weak N—H...O and C—H...O intermolecular interactions, forming chains along [011].

  4. 22 CFR 212.36 - Denial of request for access to records.

    Science.gov (United States)

    2010-04-01

    ... a request for records may be made, initially, only by the Chief, Customer Outreach and Oversight... Administrative Services, Bureau for Management, Room 803, SA-2, Agency for International Development, 21st and Virginia Ave., N.W., Washington, D.C., 20523-0217. (2) In order for the Agency to make a timely response to...

  5. 23 CFR 971.212 - Federal lands safety management system (SMS).

    Science.gov (United States)

    2010-04-01

    ... that includes: (i) Accident records with detail for analysis such as accident type using standard... classification (as appropriate); and (iv) Accident rates by customary criteria such as location, roadway... hazards and potential hazards. (3) Identification of focal points for all contacts at State, regional...

  6. Bromido(2-{1-[2-(piperidin-1-ylethylimino]ethyl}phenolatocopper(II

    Directory of Open Access Journals (Sweden)

    Xiao-Fan Zhao

    2010-08-01

    Full Text Available In the title complex, [CuBr(C15H21N2O], the CuII atom is coordinated by one phenolate O, one imine N and one amine N atom of the tridentate Schiff base ligand and by one bromide ion, resulting in a distorted CuBrN2O square-planar geometry for the metal ion, with the N atoms in a cis conformation.

  7. (2-Acetylphenolato(2-{1-[2-(morpholin-4-ylethylimino]ethyl}phenolato(thiocyanato-κNcobalt(III

    Directory of Open Access Journals (Sweden)

    Chen-Yi Wang

    2011-07-01

    Full Text Available The title mononuclear cobalt(III complex, [Co(C14H19N2O2(C8H7O2(NCS], was obtained by the reaction of 2-acetylphenol, 2-(morpholin-4-ylethylamine, ammonium thiocyanate and cobalt nitrate in methanol. The CoIII atom is coordinated by one phenolate O, one imine N, and one amine N atom of the tridentate Schiff base ligand, two O atoms of the 2-acetylphenolate anion and one thiocyanate N atom. This results in a fairly regular fac-CoN3O3 octahedral coordination geometry for the metal ion. The dihedral angle between the two benzene rings is 88.3 (3°.

  8. 37 CFR 212.3 - Registration of claims for protection of eligible designs.

    Science.gov (United States)

    2010-07-01

    .../CON must be used for each design beyond the first appearing on Form D-VH. Each Form D-VH/CON must be accompanied by deposit material identifying the design that is the subject of the Form D-VH/CON, and the deposit material must be attached to the Form D-VH/CON. The Form D-VH and all the Form D-VH/CONs for...

  9. Exercise, Nutrition and You: An Off-Campus Course for Grades 2?12

    Science.gov (United States)

    Snyder, Ann C.; Mauzy-Melitz, Debra K.

    2005-01-01

    Since interest in science classes has declined recently and obesity, especially in youth, continues to increase, an exercise physiology-based course was taught in public parks to promote active-learning science and healthy living. The course emphasized and integrated exercise, nutrition, and health during a 3-h session. Following an introduction,…

  10. From a music therapists' diary 3/12 1996 - 2/12 1997

    DEFF Research Database (Denmark)

    Bergstrøm-Nielsen, Carl

    2009-01-01

    Graphic sketches made within a period of one year. They were made right after the sessions. Purpose was to investigate possibilities in graphic representation and to let various observarions and thoughts about the therapy become graspable. Around one third of the total quantity has been selected ...

  11. 28 CFR 2.12 - Initial hearings: Setting presumptive release dates.

    Science.gov (United States)

    2010-07-01

    ..., SUPERVISION AND RECOMMITMENT OF PRISONERS, YOUTH OFFENDERS, AND JUVENILE DELINQUENTS United States Code... thereafter as practicable; except that in a case of a prisoner with a minimum term of parole ineligibility of... hearing; (2) set an effective date of parole; or (3) continue the prisoner to a fifteen year...

  12. 36 CFR 212.10 - Maximum economy National Forest System roads.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Maximum economy National... economy National Forest System roads. The Chief may acquire, construct, reconstruct, improve, and maintain... Forest Service in locations and according to specifications which will permit maximum economy in...

  13. 22 CFR 212.38 - Predisclosure notification procedures for confidential commercial information.

    Science.gov (United States)

    2010-04-01

    ... shall not be disclosed pursuant to a FOIA request except in accordance with this section. For purposes... substantial competitive harm. (2) Submitter means any person or entity who provides confidential commercial... has not been disclosed to the public. (5) Whenever the Agency provides notice to the submitter in...

  14. 8 CFR 1212.3 - Application for the exercise of discretion under former section 212(c).

    Science.gov (United States)

    2010-01-01

    ... April 24, 1996. Section 440(d) of Antiterrorism and Effective Death Penalty Act of 1996 (AEDPA) shall... Effective Death Penalty Act of 1996, with respect to a conviction if the alien pleaded guilty or nolo... 440(d) of the Antiterrorism and Effective Death Penalty Act of 1996, with respect to a conviction...

  15. 22 CFR 212.35 - Schedule of fees and method of payment for services rendered.

    Science.gov (United States)

    2010-04-01

    .... Moreover, as traditional methods of news delivery evolve (e.g., electronic dissemination of newspapers through telecommunications services), such alternative media would be included in this category. In the... request down into a series of requests for the purpose of evading the assessment of fees, the Agency...

  16. Yeast Interacting Proteins Database: YHR005C, YOR212W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available 18p to dampen signaling; may recruit Rho1p to the polarized growth site during ma...activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polar...ized growth site during mating; contains WD40 repeats Rows with this prey as prey (

  17. Yeast Interacting Proteins Database: YJR086W, YOR212W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats Rows with this prey as p...ith Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized grow

  18. Q-switched Ho:Lu2O3 laser at 2.12 μm.

    Science.gov (United States)

    Lamrini, Samir; Koopmann, Philipp; Scholle, Karsten; Fuhrberg, Peter

    2013-06-01

    We report on a Q-switched Ho:Lu2O3 laser resonantly pumped by a GaSb-based laser diode stack at 1.9 μm. The maximum output energy extracted from the compact plano-plano acousto-optically Q-switched resonator was 8 mJ at a 100 Hz pulse repetition rate, while the peak power was 40 kW. The laser wavelength was 2.124 μm.

  19. Vocabulary of signs and symptoms of the Musculoskeletal System, Vol. II: Medical imaging signs. Terminology bulletin No. 212

    Energy Technology Data Exchange (ETDEWEB)

    Lussier, A.; Beauregard, G.; Dionne, S.

    1992-01-01

    This vocabulary is the second of four on the musculoskeletal system. It is compiled from recommended works and deals mainly with radiological aspects. It also includes fractures and diseases, imaging techniques, roentgenographic positions, angles, lines, and indexes. About 500 concepts are listed in English and French with each entry including the main entry term, synonym(s), textual support, and notes.

  20. Background information and biorefinery status, potential and Sustainability: Task 2.1.2 Market and Consumers; Carbohydrates

    OpenAIRE

    Bos, H.L.; Harmsen, P.F.H.; Annevelink, E.

    2010-01-01

    This report was produced to give an overview of present and future market for biorefinery products based on carbohydrates. Various studies show that there is a wealth of possible molecules and products that can be produced from carbohydrates. Carbohydrates already find significant application in starch products and cellulose plastics and fibres. However, for a biorefinery to operate in an economically sustainable way, applications for (preferably all) biomass ingredients need to be found. Pre...

  1. Background information and biorefinery status, potential and Sustainability: Task 2.1.2 Market and Consumers; Carbohydrates

    NARCIS (Netherlands)

    Bos, H.L.; Harmsen, P.F.H.; Annevelink, E.

    2010-01-01

    This report was produced to give an overview of present and future market for biorefinery products based on carbohydrates. Various studies show that there is a wealth of possible molecules and products that can be produced from carbohydrates. Carbohydrates already find significant application in sta

  2. Oahu Hyperspectral Imagery 2000 (212-0612-332211) - Visual Interpretation from Remote Sensing Imagery Main Eight Hawaiian Islands

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort between the National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment,...

  3. CALiPER Report 21.2. Linear (T8) LED Lamp Performance in Five Types of Recessed Troffers

    Energy Technology Data Exchange (ETDEWEB)

    None

    2014-05-01

    Although lensed troffers are numerous, there are many other types of optical systems as well. This report looks at the performance of three linear (T8) LED lamps—chosen primarily based on their luminous intensity distributions (narrow, medium, and wide beam angles)—as well as a benchmark fluorescent lamp in five different troffer types. Also included are the results of a subjective evaluation. Results show that linear (T8) LED lamps can improve luminaire efficiency in K12-lensed and parabolic-louvered troffers, effect little change in volumetric and high-performance diffuse-lensed type luminaires, but reduce efficiency in recessed indirect troffers. These changes can be accompanied by visual appearance and visual comfort consequences, especially when LED lamps with clear lenses and narrow distributions are installed. Linear (T8) LED lamps with diffuse apertures exhibited wider beam angles, performed more similarly to fluorescent lamps, and received better ratings from observers. Guidance is provided on which luminaires are the best candidates for retrofitting with linear (T8) LED lamps.

  4. 32 CFR Appendix A to Part 212 - Non-Federal Entities Having Statutory Authorization for Particular Support

    Science.gov (United States)

    2010-07-01

    ..., Code of Federal Regulations (CFR), part 230. United Service Organization Section 220101 of title 36, U.S.C. Title 32, CFR, part 213. Memorandum of Understanding (MOU) between DoD and the United Service... Campaign Executive Order 12353. Title 5, CFR, part 950. DoD Instruction 5035.1. DoD Instruction...

  5. 21 CFR 212.1 - What are the meanings of the technical terms used in these regulations?

    Science.gov (United States)

    2010-04-01

    ... section. PET drug product means a finished dosage form of a PET drug, whether or not in association with... the Federal Food, Drug, and Cosmetic Act, as amended (21 U.S.C. 321 et seq.). Active pharmaceutical... active pharmaceutical ingredient. In-process material means any material fabricated, compounded,...

  6. 212例男性不育症患者的血清Prolactin RIA观察

    Institute of Scientific and Technical Information of China (English)

    乐德昌

    1995-01-01

    泌乳素(Prolactin,PRL)亦称催乳素。是腺垂体分泌的一种蛋白质激素,由198个氨基酸组成的多肽链,分子量为21500。在1988年由Vanden,Laan等首先在国际上报道用核医学技术建立起放射免疫分析方法(RIA)测定人血清垂体泌乳素,从此开拓了内分秘学、妇科学、

  7. 76 FR 68301 - Airworthiness Directives; Bell Helicopter Textron, Inc. (Bell), Model 205A-1, 205B, 210, and 212...

    Science.gov (United States)

    2011-11-04

    ... using a 3x power or higher magnifying glass. That AD was prompted by two reports of fatigue cracks on... surfaces of each affected blade with an alcohol-soaked cloth. This is required immediately before performing a visual inspection using a 3x or higher magnifying glass and a bright light to detect an...

  8. Wereldsteden uit de zestiende eeuw: de Civitates orbis terrarum van Braun en Hogenberg (t fol 212 Rar)

    NARCIS (Netherlands)

    Krogt, Peter van der

    2009-01-01

    ‘De Civitates is een van de grote boeken van de wereld [...] een fantastisch compendium over het Europese leven in de zestiende eeuw, [...] het geeft een visuele gedrukte getuigenis van middeleeuws Europa, en is een van de meest waardevolle bronnen voor de student en onderzoeker van deze tijdperken’

  9. Radiogenic Side Effects After Hypofractionated Stereotactic Photon Radiotherapy of Choroidal Melanoma in 212 Patients Treated Between 1997 and 2007

    Energy Technology Data Exchange (ETDEWEB)

    Dunavoelgyi, Roman [Department of Ophthalmology, Medical University of Vienna, Vienna (Austria); Dieckmann, Karin [Department of Radiology, Medical University of Vienna, Vienna (Austria); Gleiss, Andreas [Section of Clinical Biometrics, Medical University of Vienna, Vienna (Austria); Sacu, Stefan; Kircher, Karl; Georgopoulos, Michael [Department of Ophthalmology, Medical University of Vienna, Vienna (Austria); Georg, Dietmar [Department of Radiology, Medical University of Vienna, Vienna (Austria); Zehetmayer, Martin, E-mail: martin.zehetmayer@meduniwien.ac.at [Department of Ophthalmology, Medical University of Vienna, Vienna (Austria); Poetter, Richard [Department of Radiology, Medical University of Vienna, Vienna (Austria)

    2012-05-01

    Purpose: To evaluate side effects of hypofractionated stereotactic photon radiotherapy for patients with choroidal melanoma. Patients and Methods: Two hundred and twelve patients with choroidal melanoma unsuitable for ruthenium-106 brachytherapy or local resection were treated stereotactically at the Medical University of Vienna between 1997 and 2007 with a Linac with 6-MV photon beams in five fractions with 10, 12, or 14 Gy per fraction. Examinations for radiogenic side effects were performed at baseline and every 3 months in the first 2 years, then every 6 months until 5 years and then once a year thereafter until 10 years after radiotherapy. Adverse side effects were assessed using slit-lamp examination, funduscopy, gonioscopy, tonometry, and, if necessary, fundus photography and fluorescein angiography. Evaluations of incidence of side effects are based on an actuarial analysis. Results: One hundred and eighty-nine (89.2%) and 168 (79.2%) of the tumors were within 3 mm of the macula and the optic disc, respectively. The five most common radiotherapy side effects were retinopathy and optic neuropathy (114 cases and 107 cases, respectively), cataract development (87 cases), neovascular glaucoma (46 cases), and corneal epithelium defects (41 cases). In total, 33.6%, 38.5%, 51.2%, 75.5%, and 77.6% of the patients were free of any radiation retinopathy, optic neuropathy, cataract, neovascular glaucoma, or corneal epithelium defects 5 years after radiotherapy, respectively. Conclusion: In centrally located choroidal melanoma hypofractionated stereotactic photon radiotherapy shows a low to moderate rate of adverse long-term side effects comparable with those after proton beam radiotherapy. Future fractionation schemes should seek to further reduce adverse side effects rate while maintaining excellent local tumor control.

  10. 2.1.2. A Novel Lead(II MOF Constructed Through 2-Fold Non-linear FDA as Linker

    Directory of Open Access Journals (Sweden)

    Ya-feng Li*, Yan Liu, Wen-yuan Gao, Jing-jing Lu

    2014-04-01

    Full Text Available A unique three-dimensional metal-organic framework —— Pb(FDA (FDA = furan-2,5-dicarboxylic acid has been hydrothermally synthesized and structurally determined by single-crystal X-ray analysis. The crystal is of tetragonal system, space group I41/a, C6H2O5Pb, Mr = 361.27, a = 13.863(2 Å, b = 13.863(2 Å, c = 13.998(3 Å, V = 2690.0(8 Å3, Z = 16, Dc = 3.568 g/cm3, F(000 = 2560, ?(MuK? = 25.060 mm-1, Rint = 7.32%, R1 = 2.59% and wR1 = 5.88% for 1543 observed reflections with I > 2?(I. Pb cation is six-coordinated by oxygens of FDA, showing the hemi directed geometry. Pb cations are connected into zigzag chain along b direction in bc planar by FDA, which is furthermore linked to three-dimensional framework with very tiny aperture by bridged carboxyl oxygens of FDA. The elemental analyses, PXRD, FTIR spectra and the thermal stability have been structurally characterized.

  11. Assistive Technology for Infants, Toddlers, and Young Children with Disabilities. PACER Center ACTion Information Sheets: PHP-c212

    Science.gov (United States)

    PACER Center, 2014

    2014-01-01

    Research shows that assistive technology (AT) can help young children with disabilities to learn developmental skills. Its use may help infants and toddlers to improve in many areas such as: (1) social skills including sharing and taking turns; (2) communication skills; (3) attention span; (4) fine and gross motor skills; and (5) self confidence…

  12. {2-[1-(2-Amino-2-methylpropyliminoethyl]phenolato-κ3N,N′,O}dioxidovanadium(V

    Directory of Open Access Journals (Sweden)

    Artur Sikorski

    2009-02-01

    Full Text Available In the crystal structure of the title compound, [V(C12H17N2OO2], the vanadium(V centre is five-coordinate in a distorted square-pyramidal environment. The three atoms of the deprotonated Schiff base and a double-bonded O atom comprise the basal plane. N—H...O hydrogen bonds lead to a zigzag chain structure parallel to [001].

  13. 9,10-Bis{2-[1-(2-pyridylmethylimidazolium-3-yl]ethoxy}anthracene bis(hexafluoridophosphate

    Directory of Open Access Journals (Sweden)

    Li-Hui Guo

    2008-01-01

    Full Text Available The cation of the title compound, C36H34N6O22+·2PF6−, lies across a crystallographic inversion centre. The imidazole and pyridine rings form dihedral angles of 82.28 (5° and 11.87 (7°, respectively, with the anthracene ring system. The crystal packing is stabilized by π–π interactions between the pyridine ring and the central ring of anthracene, with a ring centroid–centroid distance of 3.684 (3 Å. The PF6− anion is disordered over three different positions with occupancies of 0.284 (6, 0.354 (8 and 0.362 (9.

  14. N-Ethyl-2-[1-(2-hy-droxy-4-methyl-phen-yl)ethyl-idene]hydrazinecarbo-thio-amide.

    Science.gov (United States)

    Anderson, Brian J; Hall, Jeffrey R; Jasinski, Jerry P

    2014-06-01

    The title compound, C12H17N3OS, crystallizes with two independent mol-ecules (A and B) in the asymmetric unit. The dihedral angle between the mean planes of the benzene ring and the hydrazinecarbo-thio-amide group are 6.9 (4) and 37.2 (5)° in mol-ecules A and B, respectively. An intra-molecular O-H⋯N hydrogen bond is observed in each mol-ecule. This serves to maintain an approximately planar conformation for mol-ecule A, but leaves a significant twist between these two groups in mol-ecule B. In the crystal, a weak N-H⋯S inter-action is observed, forming inversion dimers among the B mol-ecules and resulting in an R 2 (2)(8) motif. These dimers are further inter-connected by weak N-H⋯O and C-H⋯O inter-molecular inter-actions, forming chains along [011].

  15. How Are Middle School Climate and Academic Performance Related across Schools and over Time? REL 2017-212

    Science.gov (United States)

    Voight, Adam; Hanson, Thomas

    2017-01-01

    A growing number of educators concur that, in order to improve student academic performance, schools need to focus not only on students' academic needs but also on their social, emotional, and material needs (Piscatelli & Lee, 2011). As a result, school climate--the social, emotional, and physical characteristics of a school community (Cohen,…

  16. 40 CFR 421.212 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Science.gov (United States)

    2010-07-01

    ... technology currently available. Except as provided in 40 CFR 125.30 through 125.32, any existing point source... 678.800 Flouride 405.400 230.500 Total suspended solids 474.900 225.900 pH (1) (1) 1 Within the...

  17. 21 CFR 212.70 - What controls and acceptance criteria must I have for my finished PET drug products?

    Science.gov (United States)

    2010-04-01

    ... identity, strength, quality, purity, and, if appropriate, sterility and pyrogens. (b) Test procedures... release but must be started within 30 hours after completion of production. The 30-hour requirement may be...; (ii) You determine that all other acceptance criteria are met; (iii) You retain a reserve sample of...

  18. Di-μ1,1-azido-bis[(2-{1-[2-(isopropylaminoethylimino]ethyl}phenolatocopper(II

    Directory of Open Access Journals (Sweden)

    He-Bing Li

    2010-06-01

    Full Text Available In the centrosymmetric binuclear title complex, [Cu2(C13H19N2O2(N32], the CuII atom adopts an elongated CuON4 square-based pyramidal coordination geometry, arising from the N,N′,O-tridentate ligand and two bridging end-on azide anions. The O atom is in the basal plane, one of the azide N atoms is in the apical site and the Cu...Cu separation is 3.2365 (3 Å. A pair of intramolecular N—H...O hydrogen bonds helps to establish the molecular conformation.

  19. Dollar Summary of Federal Supply Classification and Service Category by Company, FY 85. Part 3 (R212-S208).

    Science.gov (United States)

    1985-01-01

    U () ) L() () Q C . > > Wala 1 -1 -. A -- . 1 1 - jj -1 -1 - 1 - i - alaJ C c 4 < - C 4C- C <C I C o C O 1C- (A U) a z Z z Z Z Z ala 00 0 ala4 II .14...34 " - a ao a -Z I. a ma00 0 0 w 0 0 0 U. n U w L w w II w w w aw w w U. 0 m < L I.. L. w . U I w w . wala . IL IL .. LLI~~~ 0 0 )000 000000 00000 0 mc...V M V S I ~ ~ 4 N ~4 -4C CV) (0N .qalN - - ana I a 40 a 44 a IL I J - J - J - J j WA a1a a c la a cc ae 1ma0 0 0 0 a a a a, W ala wala 0 0 Na a0 00

  20. Observations of C3H2 (2(12) - 1(01)) toward the Sagittarius A molecular cloud

    Science.gov (United States)

    Lee, C. W.; Minh, Y. C.; Irvine, W. M.

    1993-01-01

    We have mapped the C3H2 2(12)-1(01) transition line toward the Sgr A molecular cloud on a 1' grid spacing and derived C3H2 column densities of 3 approximately 7 x 10(14) cm-2 for molecular clouds of Sgr A. The fractional abundances of C3H2 relative to H2 are obtained to be 3 approximately 6 x 10(-9), which are slightly lower than that for the cold dark cloud TMC-1 but are enhanced by factors of 5-60 compared to those for Sgr B2 and the Orion extended ridge. We also estimate from the C3H2 column densities total masses of approximately 10(6) M(solar) for two clouds (M - 0.13-0.08 and M - 0.02-0.07), which are thought to be close to the virial equilibrium. We suggest that the large abundance of C3H2 in Sgr A may be partly due to the activities of the Galactic center.

  1. Factors That Predict Satisfaction With Medical Care: Data From 27,212 Injured Workers Surveyed for 14 Years.

    Science.gov (United States)

    Joshi, Anand B; McCormick, Zachary L; Sully, Kate; Garvan, Cynthia; Plastaras, Christopher T

    2016-01-01

    the aim of this study is to report satisfaction rates and identify factors predicting satisfaction in the Pennsylvania Workers' Compensation (WC) system. Cross-sectional survey data on satisfaction and care experience of injured workers were collected annually for 14 years with 27,712 injured workers responding. The overall response rate was 19.44%. The overall proportion of satisfied workers was 83.97% with a range of 80.43% to 88.13%. Obtaining a correct initial diagnosis [odds ratio (OR) = 4.26], explanation of WC rights (OR = 2.58), and physician explanation of treatment options (OR = 1.83) most strongly predicted satisfaction. Injured worker satisfaction with their medical care exceeded 80% in all years surveyed. Patient perception of a correct initial diagnosis, explanation of WC rights, and explanation of treatment options were the strongest predictors of satisfaction during the 14-year study period.

  2. 8 CFR 212.2 - Consent to reapply for admission after deportation, removal or departure at Government expense.

    Science.gov (United States)

    2010-01-01

    ..., removal or departure at Government expense. (a) Evidence. Any alien who has been deported or removed from... who has been deported or removed from the United States and is applying for a visa, admission to the... alien deported, or removed 2 or more times, or at any time after deportation or removal in the case...

  3. Is asthma in 2-12 year-old children associated with physician-attended recurrent upper respiratory tract infections?

    NARCIS (Netherlands)

    Hak, Eelko; Rovers, Maroeska M; Sachs, Alfred P E; Stalman, Wim A B; Verheij, Theo J M

    2003-01-01

    In a prevalence study, we evaluated whether recurrent physician-attended URTI episodes are more common in asthmatic children as compared to age- and gender-matched controls. URTI proneness, defined as > or = 5 episodes of rhinitis/pharyngitis, sinusitis, laryngitis/tracheitis or otitis media in a 24

  4. The effect of spinally administered WIN 55,212-2, a cannabinoid agonist, on thermal pain sensitivity in diabetic rats

    Directory of Open Access Journals (Sweden)

    Samane Jahanabadi

    2016-04-01

    Conclusion: These data show that cannabinoids have potent antinociceptive effects through direct actions in the spinal dorsal horn of nociceptive pathway. This suggests that intrathecally administered cannabinoids may offer hopeful strategies for the treatment of diabetic neuropathic pain.

  5. 2(1/2)D(FD)~2TD Method and Its Application to an Antenna in Water

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    212D(FD)2TDMethodandItsApplicationtoanAntennainWaterQingAnyongLiJingRenLangInstituteofElectromagneticFieldandMicrowaveTechnol...

  6. 48 CFR 52.212-5 - Contract Terms and Conditions Required To Implement Statutes or Executive Orders-Commercial Items.

    Science.gov (United States)

    2010-10-01

    ...). ___(2) 52.203-13, Contractor Code of Business Ethics and Conduct (APR 2010)(Pub. L. 110-252, Title VI... required by the clause— (i) 52.203-13, Contractor Code of Business Ethics and Conduct (APR 2010) (Pub. L... Business Ethics and Conduct (APR 2010) (Pub. L. 110-252, Title VI, Chapter 1 (41 U.S.C. 251 note)). (B)...

  7. Oahu Hyperspectral Imagery 2000 (212-0612-272217) - Visual Interpretation from Remote Sensing Imagery Main Eight Hawaiian Islands

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort between the National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment,...

  8. 50 CFR 226.212 - Critical habitat for 13 Evolutionarily Significant Units (ESUs) of salmon and steelhead...

    Science.gov (United States)

    2010-10-01

    ...) Land subject to the Green Diamond Company Habitat Conservation Plan. Critical habitat is excluded on... Green Diamond Resources Company (formerly Simpson Timber Company). (h) Land subject to the West Fork... (48.7841, -122.0293); Middle Fork Nooksack River (48.7249, -121.8999); Porter Creek (48.7951,...

  9. Integracion del Guiador Excentrico AL Telescopio UNAM212 Y SU Repercusion EN Las Imagenes Obtenidas con Diversos Instrumentos

    Science.gov (United States)

    Cobos, F. J.; Langarica, R.

    1987-05-01

    A change in one dimension of the offset guider container is proposed. This modification based on image analysis is carried out in order to guarantee its universality of usage with minimal variations in the original design and minimal alterations in the actual configuration of the peripherical instruments. The performance of the offset guider with all periphericals is expected to be of better (or at least similar) quality than the one presently obtained.

  10. 22 CFR 212.41 - Exemptions from publication and disclosure requirements of subparts B, C, and D.

    Science.gov (United States)

    2010-04-01

    ... national defense or foreign policy and are in fact properly classified pursuant to such Executive Order; (b... interfere with enforcement proceedings; (2) Would deprive a person of a right to a fair trial or an... confidential basis, and, in the case of a record or information compiled by a criminal law...

  11. Assistive Technology for Infants, Toddlers, and Young Children with Disabilities. PACER Center ACTion Information Sheets: PHP-c212

    Science.gov (United States)

    PACER Center, 2014

    2014-01-01

    Research shows that assistive technology (AT) can help young children with disabilities to learn developmental skills. Its use may help infants and toddlers to improve in many areas such as: (1) social skills including sharing and taking turns; (2) communication skills; (3) attention span; (4) fine and gross motor skills; and (5) self confidence…

  12. Accurate dipole polarizabilities for water clusters n=2-12 at the coupled-cluster level of theory and benchmarking of various density functionals

    Energy Technology Data Exchange (ETDEWEB)

    Hammond, Jeffrey R.; Govind, Niranjan; Kowalski, Karol; Autschbach, Jochen; Xantheas, Sotiris S.

    2009-12-07

    The static dipole polarizabilities of water clusters are determined at the Coupled-Cluster level of theory (CCSD). For the dipole polarizability of the water monomer it was determined that the role of the basis set is even more important than that of electron correlation and that the basis set augmentation converges with two sets of diffuse functions. The CCSD results are used to benchmark a variety of density functionals while the performance of several families of basis sets (Dunning, Pople and Sadlej) in producing accurate values for the polarizabilities was also examined. The Sadlej family of basis sets was found to produce accurate results when compared to the ones obtained with the much larger Dunning basis sets. It was furthermore determined that the PBE0 density functional with the aug-cc-pVDZ basis set produces overall remarkably accurate polarizabilities at a moderate computational cost.

  13. Inhibition of miRNA-212/132 improves the reprogramming of fibroblasts into induced pluripotent stem cells by de-repressing important epigenetic remodelling factors

    Directory of Open Access Journals (Sweden)

    Nils Pfaff

    2017-04-01

    Thus, conducting a full library miRNA screen we here describe a miRNA family, which markedly reduces generation of iPSC and upon inhibition in turn enhances reprogramming. These miRNAs, at least in part, exert their functions through repression of the epigenetic modulators p300 and Jarid1a, highlighting these two molecules as an endogenous epigenetic roadblock during iPSC generation.

  14. Differential effects of repeated low dose treatment with the cannabinoid agonist WIN 55,212-2 in experimental models of bone cancer pain and neuropathic pain

    DEFF Research Database (Denmark)

    Hald, Andreas; Ding, Ming; Egerod, Kristoffer Lihme;

    2008-01-01

    . Furthermore, this treatment strategy was not found to induce measurable CNS related side effects or tolerance. Cancer cell viability assays and bone volume fraction assessed by micro computed tomography (microCT) demonstrated that these effects were not due to changes in cancer progression. The difference...

  15. 212. Incidencia de complicaciones graves en trasplantes cardíacos urgentes y no urgentes. 10 años de experiencia

    Directory of Open Access Journals (Sweden)

    L. Donate Bertolín

    2010-01-01

    Conclusiones: En nuestra serie, el trasplante urgente no presentó una mayor mortalidad perioperatoria. Sin embargo, observamos mayor número de complicaciones graves, asociadas a mortalidad a más largo plazo, como FAI, infección bacteriana y ventilación mecánica prolongada.

  16. Poly[[μ2-1,2-bis(imidazol-1-ylmethylbenzene](μ2-cyclohexane-1,4-dicarboxylatocobalt(II

    Directory of Open Access Journals (Sweden)

    Min Xing

    2010-03-01

    Full Text Available In the the title compound, [Co(C8H10O4(C14H14N4]n, the CoII atom is four-coordinated by two N atoms from two different 1,2-bis(imidazol-1-ylmethylbenzene ligands and two carboxylate O atoms from two different cyclohexane-1,4-dicarboxylate anions in a tetrahedral coordination geometry. The resulting structure is a two-dimensional polymer with layers in the (100 plane.

  17. 76 FR 67051 - Airworthiness Directives; Bell Helicopter Textron, Inc. Model 204B, 205A, 205A-1, 205B, 210, 212...

    Science.gov (United States)

    2011-10-31

    .... (BHT) Model helicopters with certain tail rotor blades (blades). The superseded AD requires, before... 2010-26-52), which was sent to all known owners and operators of BHT Model 204B, 205A, 205A-1, 205B.... Relevant Service Information We have reviewed the following revised BHT Alert Service Bulletins (ASBs), all...

  18. 76 FR 35334 - Airworthiness Directives; Bell Helicopter Textron, Inc. Model 205A, 205A-1, 205B, 212, 412, 412CF...

    Science.gov (United States)

    2011-06-17

    ... directive (AD) for the specified Bell Helicopter Textron, Inc. (BHT) model helicopters with tail rotor (T R.... SUPPLEMENTARY INFORMATION: This amendment adopts a new AD for the specified BHT model helicopters with an... blade, and subsequent loss of control of the helicopter. We have reviewed the following BHT Alert...

  19. Selective [3+1] Fragmentations of P4 by \\01cP\\01d Transfer from a Lewis Acid Stabilized [RP4]\\212 Butterfly Anion

    NARCIS (Netherlands)

    Borger, Jaap E.; Ehlers, Andreas W.; Lutz, Martin|info:eu-repo/dai/nl/304828971; Slootweg, J. Chris; Lammertsma, Koop

    2017-01-01

    Two [3+1] fragmentations of the Lewis acid stabilized bicyclo[1.1.0]tetraphosphabutanide Li[Mes*P4⋅ BPh3] (Mes*=2,4,6-tBu3C6H2) are reported. The reactions proceed by extrusion of a P1 fragment, induced by either an imidazolium salt or phenylisocyanate, with release of the transient triphosphirene

  20. Topologia differenziale Lectures Given At The Centro Internazionale Matematico Estivo (CIME) Held In Urbino (Pesaro), Italy, July 2-12, 1962

    CERN Document Server

    Vesentini, E

    2011-01-01

    J. Cerf: Invariants des paires d espaces. Applications la topologie differentielle.- A. H fliger: Vari t?'s feuillet es.- M.A. Kervaire: La m thode de Pontryagin pour la classification des applications sur une sph re.- S. Smale: Stable manifolds for differential equations and diffeomorphisms.

  1. Long-term disease-modifying effect of the endocannabinoid agonist WIN55,212-2 in a rat model of audiogenic epilepsy

    NARCIS (Netherlands)

    Vinogradova, L.V.; Rijn, C.M. van

    2015-01-01

    BACKGROUND: Modulation of the endocannabinoid (eCB) transmission is a promising approach to treating epilepsy. Animal models can be used to investigate this approach. Krushinsky-Molodkina (KM) rats have, genetically, audiogenic epilepsy. Moreover, in these animals, repeated induction of audiogenic s

  2. Heterospin systems constructed from [Cu2Ln]3+ and [Ni(mnt)2]1-,2- Tectons: First 3p-3d-4f complexes (mnt = maleonitriledithiolato).

    Science.gov (United States)

    Madalan, Augustin M; Avarvari, Narcis; Fourmigué, Marc; Clérac, Rodolphe; Chibotaru, Liviu F; Clima, Sergiu; Andruh, Marius

    2008-02-04

    New heterospin complexes have been obtained by combining the binuclear complexes [{Cu(H(2)O)L(1)}Ln(O(2)NO)(3)] or [{CuL(2)}Ln(O(2)NO)(3)] (L(1) = N,N'-propylene-di(3-methoxysalicylideneiminato); L(2) = N,N'-ethylene-di(3-methoxysalicylideneiminato); Ln = Gd(3+), Sm(3+), Tb(3+)), with the mononuclear [CuL(1)(2)] and the nickel dithiolene complexes [Ni(mnt)(2)](q)- (q = 1, 2; mnt = maleonitriledithiolate), as follows: (1)infinity[{CuL(1)}(2)Ln(O(2)NO){Ni(mnt)(2)}].Solv.CH(3)CN (Ln = Gd(3+), Solv = CH(3)OH (1), Ln = Sm(3+), Solv = CH(3)CN (2)) and [{(CH(3)OH)CuL(2)}(2)Sm(O(2)NO)][Ni(mnt)(2)] (3) with [Ni(mnt)2]2-, [{(CH(3)CN)CuL(1)}(2)Ln(H(2)O)][Ni(mnt)(2)]3.2CH(3)CN (Ln = Gd(3+) (4), Sm(3+) (5), Tb(3+) (6)), and [{(CH(3)OH)CuL(2)}{CuL(2)}Gd(O(2)NO){Ni(mnt)(2)}][Ni(mnt)(2)].CH(2)Cl(2) (7) with [Ni(mnt))(2]*-. Trinuclear, almost linear, [CuLnCu] motifs are found in all the compounds. In the isostructural 1 and 2, two trans cyano groups from a [Ni(mnt)2]2- unit bridge two trimetallic nodes through axial coordination to the Cu centers, thus leading to the establishment of infinite chains. 3 is an ionic compound, containing discrete [{(CH(3)OH)CuL(2)}(2)Sm(O(2)NO)](2+) cations and [Ni(mnt)(2)](2-) anions. Within the series 4-6, layers of discrete [CuLnCu](3+) motifs alternate with stacks of interacting [Ni(mnt)(2)](*-) radical anions, for which two overlap modes, providing two different types of stacks, can be disclosed. The strength of the intermolecular interactions between the open-shell species is estimated through extended Hückel calculations. In compound 7, [Ni(mnt)(2)](*-) radical anions coordinate group one of the Cu centers of a trinuclear [Cu(2)Gd] motif through a CN, while discrete [Ni(mnt)(2)](*-) units are also present, overlapping in between, but also with the coordinated ones. Furthermore, the [Cu(2)Gd] moieties dimerize each other upon linkage by two nitrato groups, both acting as chelate toward the gadolinium ion from one unit and monodentate toward a Cu ion from the other unit. The magnetic properties of the gadolinium-containing complexes have been determined. Ferromagnetic exchange interactions within the trinuclear [Cu(2)Gd] motifs occur. In the compounds 4 and 7, the [Ni(mnt)(2)](*-) radical anions contribution to the magnetization is clearly observed in the high-temperature regime, and most of it vanishes upon temperature decrease, very likely because of the rather strong antiferromagnetic exchange interactions between the open-shell species. The extent of the exchange interaction in the compound 7, which was found to be antiferromagnetic, between the coordinated Cu center and the corresponding [Ni(mnt)(2)](*-) radical anion, bearing mostly a 3p spin type, was estimated through CASSCF/CASPT2 calculations. Compound 6 exhibits a slow relaxation of the magnetization.

  3. DOE CALiPER Program, Report 21.2: Linear (T8) LED Lamp Performance in Five Types of Recessed Troffers

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Naomi J.; Perrin, Tess E.; Royer, Michael P.; Wilkerson, Andrea M.; Beeson, Tracy A.

    2014-05-20

    Although lensed troffers are numerous, there are many other types of optical systems as well. This report looked at the performance of three linear (T8) LED lamps chosen primarily based on their luminous intensity distributions (narrow, medium, and wide beam angles) as well as a benchmark fluorescent lamp in five different troffer types. Also included are the results of a subjective evaluation. Results show that linear (T8) LED lamps can improve luminaire efficiency in K12-lensed and parabolic-louvered troffers, effect little change in volumetric and high-performance diffuse-lensed type luminaires, but reduce efficiency in recessed indirect troffers. These changes can be accompanied by visual appearance and visual comfort consequences, especially when LED lamps with clear lenses and narrow distributions are installed. Linear (T8) LED lamps with diffuse apertures exhibited wider beam angles, performed more similarly to fluorescent lamps, and received better ratings from observers. Guidance is provided on which luminaires are the best candidates for retrofitting with linear (T8) LED lamps.

  4. Corrigendum to "An integrated model for dune morphology and sand fluxes on Mars" [Earth Planet. Sci. Lett. 457 (2017) 204-212

    Science.gov (United States)

    Runyon, K. D.; Bridges, N. T.; Ayoub, F.; Newman, C. E.; Quade, J. J.

    2017-02-01

    The authors regret that some of the X and Y data were de-correlated in a Matlab script used to generate Fig. 5a (top left pane). They confirm this is an isolated incident and does not change the discussion or conclusion of the paper, nor does it change the message of the figure.

  5. First examples of a modulated bridging mu(2)-1:2kappan-triazine in double helical silver compounds. experimental and theoretical evidence.

    Science.gov (United States)

    Carranza, M Pilar; Manzano, Blanca R; Jalón, Félix A; Rodríguez, Ana M; Santos, Lucía; Moreno, Miquel

    2010-04-19

    The synthesis of several silver double helices containing bis(3,5dimethylpyrazolyl)-6-(R)-s-triazine ligands is described. The structure of two of them has been determined by X-ray difraction. Both derivatives represent the first reported examples of a new interaction mode for a triazine ring that involves a triazine N atom bridging two metal centers. Argentophilic contacts are also present. The Ag-N and Ag-Ag interactions have been demonstrated by theoretical studies, which also showed the clear influence of weak interactions with the counteranion and the effect of the symmetry of the triazine substituent. The different donor characters of these substituents allows a modulation of the strength of the bridging Ag-N(triazine) interaction. Double pi-pi stacking, anion-pi interactions, hydrogen bonds, and hydrophobic effects are observed in an unusual highly symmetrical interpenetrated three-dimensional superstructure.

  6. Why is the neutrino oscillation formula expanded in Δ m {21/2}/Δ m {31/2} still accurate near the solar resonance in matter?

    Science.gov (United States)

    Xu, Xun-Jie

    2015-10-01

    The conventional approximate formula for neutrino oscillation in matter which is obtained from the expansion in terms of the ratio of mass square differences α = Δ m 21 2 /Δ m 31 2 ≈ 0.03, first proposed by Cervera, et al. and Freund, turns out to be an accurate formula for accelerator neutrino experiments. Originally it required the neutrino energy to be well above the solar resonance to validate the expansion but it is found to be still very accurate when the formula is extrapolated to the resonance, which is practically important for the T2K experiment. This paper shows that the accuracy is guaranteed by cancellations of branch cut singularities and also, for the first time, analytically computes the actual error of the formula. The actual error implies that the original requirement can be safely removed in current experiments.

  7. About the Dissertations in Geological Sciences defended at Dissertation Committee Д 212.189.01 of the Perm State National Research University in 2013

    Directory of Open Access Journals (Sweden)

    V. A. Gershanok

    2014-03-01

    Full Text Available The article contains the information about the candidate’s dissertations in geological sciences defended at the Dissertation Committee of Perm State National Research University in 2013. The annotations of these works are presented as well.

  8. SU-E-J-212: MR Diffusion Tensor Imaging for Assessment of Tumor and Normal Brain Tissue Responses of Juvenile Pilocytic Astrocytoma Treated by Proton Therapy

    Energy Technology Data Exchange (ETDEWEB)

    Hou, P; Park, P; Li, H; Zhu, X; Mahajan, A; Grosshans, D [M.D. Anderson Cancer Center, Houston, TX (United States)

    2015-06-15

    Purpose: Diffusion tensor imaging (DTI) can measure molecular mobility at the cellular level, quantified by the apparent diffusion coefficient (ADC). DTI may also reveal axonal fiber directional information in the white matter, quantified by the fractional anisotropy (FA). Juvenile pilocytic astrocytoma (JPA) is a rare brain tumor that occurs in children and young adults. Proton therapy (PT) is increasingly used in the treatment of pediatric brain tumors including JPA. However, the response of both tumors and normal tissues to PT is currently under investigation. We report tumor and normal brain tissue responses for a pediatric case of JPA treated with PT assessed using DTI. Methods: A ten year old male with JPA of the left thalamus received passive scattered PT to a dose of 50.4 Gy (RBE) in 28 fractions. Post PT, the patient has been followed up in seven years. At each follow up, MRI imaging including DTI was performed to assess response. MR images were registered to the treatment planning CT and the GTV mapped onto each MRI. The GTV contour was then mirrored to the right side of brain through the patient’s middle line to represent normal brain tissue. ADC and FA were measured within the ROIs. Results: Proton therapy can completely spare contra lateral brain while the target volume received full prescribed dose. From a series of MRI ADC images before and after PT at different follow ups, the enhancement corresponding to GTV had nearly disappeared more than 2 years after PT. Both ADC and FA demonstrate that contralateral normal brain tissue were not affect by PT and the tumor volume reverted to normal ADC and FA values. Conclusion: DTI allowed quantitative evaluation of tumor and normal brain tissue responses to PT. Further study in a larger cohort is warranted.

  9. 77 FR 67557 - Special Conditions: ATR-GIE Avions de Transport Regional, Models ATR42-500 and ATR72-212A...

    Science.gov (United States)

    2012-11-13

    ...-engine, turbo- propeller driven. The Model ATR42-500 has a maximum takeoff weight of 41,005 pounds and an... Privacy Act Statement can be found in the Federal Register published on April 11, 2000 (65 FR...

  10. Federal Education Tax Benefits: Who Receives Them and to What Extent Do They Shape the Price of College Attendance? Stats in Brief. NCES 2012-212

    Science.gov (United States)

    Radford, Alexandria Walton; Berkner, Lutz

    2011-01-01

    This Statistics in Brief applies IRS rules and data to a nationally representative sample of 2007-08 undergraduates to estimate who received education tax benefits and looks at the extent to which these benefits shaped their price of college attendance. Key findings include: (1) Nearly one-half of all 2007-08 undergraduates were estimated to have…

  11. Using the World-Wide Web to obtain large-scale word norms: 190,212 ratings on a set of 2,654 German nouns.

    Science.gov (United States)

    Lahl, Olaf; Göritz, Anja S; Pietrowsky, Reinhard; Rosenberg, Jessica

    2009-02-01

    This article presents a new database of 2,654 German nouns rated by a sample of 3,907 subjects on three psycholinguistic attributes: concreteness, valence, and arousal. As a new means of data collection in the field of psycholinguistic research, all ratings were obtained via the Internet, using a tailored Web application. Analysis of the obtained word norms showed good agreement with two existing norm sets. A cluster analysis revealed a plausible set of four classes of nouns: abstract concepts, aversive events, pleasant activities, and physical objects. In an additional application example, we demonstrate the usefulness of the database for creating parallel word lists whose elements match as closely as possible. The complete database is available for free from ftp://ftp.uni-duesseldorf.de/pub/psycho/lahl/WWN. Moreover, the Web application used for data collection is inherently capable of collecting word norms in any language and is going to be released for public use as well.

  12. Autotrophic and heterotrophic soil respiration determined with trenching, soil CO2 fluxes and 13CO2/12CO2 concentration gradients in a boreal forest ecosystem

    Science.gov (United States)

    Pumpanen, Jukka; Shurpali, Narasinha; Kulmala, Liisa; Kolari, Pasi; Heinonsalo, Jussi

    2017-04-01

    Soil CO2 efflux forms a substantial part of the ecosystem carbon balance, and it can contribute more than half of the annual ecosystem respiration. Recently assimilated carbon which has been fixed in photosynthesis during the previous days plays an important role in soil CO2 efflux, and its contribution is seasonally variable. Moreover, the recently assimilated C has been shown to stimulate the decomposition of recalcitrant C in soil and increase the mineralization of nitrogen, the most important macronutrient limiting gross primary productivity (GPP) in boreal ecosystems. Podzolic soils, typical in boreal zone, have distinctive layers with different biological and chemical properties. The biological activity in different soil layers has large seasonal variation due to vertical gradient in temperature, soil organic matter and root biomass. Thus, the source of CO2 and its components have a vertical gradient which is seasonally variable. The contribution of recently assimilated C and its seasonal as well as spatial variation in soil are difficult to assess without disturbing the system. The most common method of partitioning soil respiration into its components is trenching which entails the roots being cut or girdling where the flow of carbohydrates from the canopy to roots has been isolated by cutting of the phloem. Other methods for determining the contribution of autotrophic (Ra) and heterotrophic (Rh) respiration components in soil CO2 efflux are pulse labelling with 13CO2 or 14CO2 or the natural abundance of 13C and/or 14C isotopes. Also differences in seasonal and short-term temperature response of soil respiration have been used to separate Ra and Rh. We compared the seasonal variation in Ra and Rh using the trenching method and differences between seasonal and short-term temperature responses of soil respiration. I addition, we estimated the vertical variation in soil biological activity using soil CO2 concentration and the natural abundance of 13C and 12C in CO2 in different soil layers in a boreal forest in Southern Finland and compared them to seasonal variation in GPP. Our results show that Ra followed a seasonal variation in GPP with a time lag of about 2 weeks. The contribution of Ra on soil CO2 efflux was largest in July and August. There was also a distinct seasonal pattern in the vertical distribution of soil CO2 concentration and the abundances of natural isotopes 13C/12C in soil CO2 which reflected the changes in biological activity in the soil profile. Our results indicate that all methods were able to distinguish seasonal variability in Ra and Rh. The soil CO2 gradient method was able to reproduce the temporal variation in soil CO2 effluxes relatively well when compared to those measured with chambers. However, variation in soil moisture also causes significant variation in soil air CO2 concentrations which interferes with the variation resulted from soil temperatures and belowground allocation of carbon from recent photosynthate. Also, the assumptions used in gradient method calculations, such as soil porosity and transport distances have to be taken into account when interpreting the results.

  13. STIR Proposal For Research Area 2.1.2 Surface Energy Balance: Transient Soil Density Impacts Land Surface Characteristics and Characterization

    Science.gov (United States)

    2015-12-22

    SECURITY CLASSIFICATION OF: Soil density is commonly treated as static in studies on land surface property dynamics. Magnitudes of errors associated...properties, and ii) evaluate impact of changing soil density on surface energy balance and heat and water transfer. Six soil properties were...ABSTRACT 2. REPORT TYPE 17. LIMITATION OF ABSTRACT 15. NUMBER OF PAGES 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 5c. PROGRAM ELEMENT

  14. Oxido[2-{(E-[((1E-{(E-2-[1-(2-oxidophenylethylidene]hydrazin-1-ylidene}(prop-2-en-1-ylsulfanylmethylimino]methyl}phenolato]vanadium(IV

    Directory of Open Access Journals (Sweden)

    Reza Takjoo

    2012-08-01

    Full Text Available The VIV atom in the title complex, [V(C19H17N3O2SO], is coordinated by two N and two O atoms of the dianionic tetradentate Schiff base ligand and the terminal oxide O atom. The N2O3 donor set defines a square-pyramidal coordination geometry with the oxide O atom in the apical site. Some buckling in the tetradentate ligand is indicated by the dihedral angle of 17.92 (19° between the six-membered chelate rings. Supramolecular chains are formed along the b axis via C—H...O contacts in the crystal. The chains are connected into a layer in the ab plane via C—H...π interactions. The atoms comprising the –SCH2—CH=CH2 and methyl substituents were found to be disordered in a 0.916 (2:0.088 (2 ratio. The crystal studied was found to be twinned by nonmerohedry with a 28.1 (4% minor twin component.

  15. 76 FR 27281 - Airworthiness Directives; Dowty Propellers Type R212/4-30-4/22 and R251/4-30-4/49 Propeller...

    Science.gov (United States)

    2011-05-11

    ..., Aerospace Engineer, Boston Aircraft Certification Office, FAA, Engine and Propeller Directorate, 12 New... Aircraft Certification Office, FAA, Engine and Propeller Directorate, 12 New England Executive Park..., Acting Manager, Engine and Propeller Directorate, Aircraft Certification Service. BILLING CODE 4910-13-P...

  16. 76 FR 61558 - Airworthiness Directives; Dowty Propellers Type R212/4-30-4/22 and R251/4-30-4/49 Propeller...

    Science.gov (United States)

    2011-10-05

    ..., Aerospace Engineer, Boston Aircraft Certification Office, FAA, Engine & Propeller Directorate, 12 New..., Manager, Engine & Propeller Directorate, Aircraft Certification Service. BILLING CODE 4910-13-P ...-01-AD; Amendment 39-16807; AD 2011-19-02] RIN 2120-AA64 Airworthiness Directives; Dowty...

  17. Poly[{μ2-1,2-bis[4-(3-pyridylpyrimidin-2-ylsulfanyl]ethane}di-μ2-cyanido-dicopper(I

    Directory of Open Access Journals (Sweden)

    Lin Cheng

    2008-07-01

    Full Text Available The asymmetric unit of the title complex, [Cu2(CN2(C20H16N6S2]n, contains one CuI cation, one cyanide ligand and half of a centrosymmetric 1,2-bis[4-(3-pyridylpyrimidin-2-ylsulfanyl]ethane (bppe ligand. The CuI atom displays a trigonal coordination geometry, being surrounded by one C atom from one cyanide anion and two N atoms from one cyanide and one bppe ligand. In the complex, each cyanide anion links two CuI atoms in a bis-monodentate mode into a zigzag [–Cu—CN–]n chain. Two parallel chains are linked by bppe ligands into a ladder chain.

  18. 10 CFR Appendix E to Part 20 - Nationally Tracked Source Thresholds

    Science.gov (United States)

    2010-01-01

    ... Category 1(TBq) Category 1(Ci) Category 2(TBq) Category 2(Ci) Actinium-227 20 540 0.2 5.4 Americium-241 60... 2 54 Strontium-90 1,000 27,000 10 270 Thorium-228 20 540 0.2 5.4 Thorium-229 20 540 0.2 5.4...

  19. I. The metabolic properties of plutonium and allied materials

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton, J.G.

    1948-05-24

    This report on the metabolic properties of plutonium and related radioactive materials presents experimental information in the following areas: radioautographic studies; tracer studies (with tables of accumulation in tissues) of actinium, radio-zirconium, technetium, radio-rubidium, radio-germanium, beryllium, and cadmium; decontamination and bone metabolism studies; and radio-chemical isolation.

  20. Monofunctionalization of Calix[4]arene Tetracarboxylic Acid at the Upper Rim with Isothiocyanate Group: First Bifunctional Chelating Agent for Alpha-Emitter Ac-225.

    Science.gov (United States)

    Chen, Xiaoyuan; Ji, Min; Fisher, Darrell R; Wai, Chien M

    1999-09-01

    A procedure is reported for synthesizing a novel, water-soluble bifunctional chelating agent derived from calix[4]arene. This chelate features tetracarboxylic acid groups at the lower rim as an actinium-225 ionophore, and an isothiocyanate functional group at the upper rim for labeling of the N-terminus of monoclonal antibodies through thiourea linkage.