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Sample records for acos api 5l

  1. Study of API 5L X70 steel corrosion processes when in contact with some Brazilian soils; Estudo dos processos de corrosao de acos API 5L X70 em contato com alguns dos solos do Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Jesus, Sergio Luis de

    2007-07-01

    Pipelines, fuel storage tanks and other metallic structures are in permanent contact and exposed to different types of soils, of horizons or layers, or of soil aggressiveness. This interaction may cause expressive damages to the environment and to the planned work. Contamination may occur due to leakage of stored products, splitting during transportation, accidents caused by pipelines without extensive maintenance. The result of these accidents could be, among others, some financial losses. In order to recognize the dynamic interactions between metallic surfaces and the environment it is crucial to have preventive actions and to develop better-applied materials. API steel 5L X70 has been used in structures of low and high pressure with high mechanical strength and corrosion and, even so, it is susceptible to etching corrosion since it is in contact with different environments from mangrove regions to industrial environments. The present case evaluated the role of 5L X70 API steel in contact with different soil horizons representative of the Brazilian soil. This investigation correlated chemical species with solute ions in soil solution, secondary and primary phase minerals besides physical and chemical characteristics as pH, electric conductivity, total dissolved solids, among others, to the results of corrosion resistance and ways of corrosion. The evaluation was carried out using x-ray diffractometry, scanning electron microscopy, total reflection x-ray fluorescence, fuel injection flow besides texture and gravimetric analyses to soil characterization and mineralogy, identification of corrosion products, soil solution analyses, evaluation of tested materials and classification of ways and types of corrosion. This was an attempt to integrate the data to a better understanding of the process involving reagents and products. The results showed that different soil horizons such as different types of analyzed soils produce specific etching in metallic structures

  2. Hardness and microstructural characterization of API 5L X70 steel pipes welded by HF/ERW process; Caracterizacao microestrutural e de dureza em tubos de aco API 5L X70 soldados pelo processo HF/ERW

    Energy Technology Data Exchange (ETDEWEB)

    Calcada, Mauricio Vieira; Voorwald, Herman Jacobus Cornelis; Nascimento, Marcelino Pereira do [UNESP, Guaratingueta, SP (Brazil). Faculdade de Engenharia

    2010-07-01

    The materials that stand in the manufacture of steel pipes are called API, that should have, high mechanical resistance, high corrosion resistance, high fatigue resistance, good weldability, and other properties. Thus, the purpose of this project was to evaluate the microstructure and hardness of welded joints by the HF/ERW process of API 5L X70 steel pipes. The microstructural analysis was performed using a surface finish with grit sizes from 220 to 25 {mu}m e polishing with diamond paste from 9 {mu}m to 0.05 {mu}m; the revelation was made with 3% Nital attack. The Vickers hardness was performed across the welded joint by 33 points to indentation. The results were: 80.5 {+-} 3.4% of ferrite and 19.5 {+-} 3.4% of perlite for microanalysis. As for hardness, the values were: 215.69 HV10 for weld line, 218.65 HV10 for ZTA and 218.95 HV10 for base metal. (author)

  3. Development of API 5L X70 and X80 in the Brazilian Vallourec and Mannesmann Tubes industry; Desenvolvimento de acos API 5L X70 e X80 na Vallourec e Mannesmann Tubes

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Julio M. Silveira e; Carvalho, Ricardo N. de [Vallourec and Mannesmann Tubes - V e M do Brasil, Belo Horizonte, MG (Brazil)

    2000-07-01

    The demand for seamless pipes with resistance higher than those for API 5L X60 and X65 grades has increased in the last years. They are traditionally used up to now for applications on rigid risers for production and exportation and line pipes for oil and gas. In the case of seamless pipes, the great challenge is to fulfill the requirements of mechanical properties for X70 and X80 grades, quenched and tempered, with values of carbon equivalent low enough to assure a good weldability. This paper presents the results obtained by Vallourec and Mannesmann Tubes - V and M do Brazil on producing seamless pipes of X70 and X80 API grades, with outside diameter lower than 8 5/8{sup (} 219,1 mm ) and wall thickness up to 0,562{sup (} 14,3 mm ). Results of tensile, Charpy notch impact resistance and hardness tests are shown. Hydrogen-Induced and Environmental Cracking tests were also carried out, since for some applications is required a good resistance to these kinds of embrittlement mechanisms. The mechanical properties, microstructure and physical characteristics of welded joints were also evaluated. The results obtained up to now are quite promising, showing good suitability to the aimed final applications. (author)

  4. Obtaining and analysis of results of fatigue and corrosion-fatigue in steel API 5L X60; Obtencao e analise de resultados de fadiga e corrosao-fadiga em aco API 5L X60

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Bruno Allison [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Programa de Pos-Graduacao em Ciencia e Engenharia de Materiais; Silva, Antonio Almeida; Santos, Fabio Gualberto Chagas [Universidade Federal de Campina Grande (UFCG), PB (Brazil)

    2008-07-01

    The corrosion process allied to the fatigue, due to repetitive efforts of several natures, is the main responsible for the damages in pipeline and offshore structures that results in the appears of located faults, and by the way can results in leaks and financial and environmental loss. This phenomenon calls corrosion-fatigue, however, it is very complex, and mainly, in what it concerns the form as this it develops in the structure. The objective of this work is to present some results of experimental fatigue tests and corrosion-fatigue accomplished with specimen that the material originated a pipeline steel API 5L X60. The tests developed in a machine which could test until 12 specimens per time. For test of corrosion-fatigue was used a cell-of-corrosion especially projected, in this way simulated an aggressive environmental condition in a corrosion conditions. With the results of tests, was possible estimate the fatigue limits of the specimen when submitted to the repeated flexing, and compare it with evaluate corrosion-fatigue graphs, that as the literature comes moved down of the curve, in relation to the fatigue curve. (author)

  5. Effect of the welding process on the microstructure and microhardness of API 5L X80 steel welded joint used for oil transportation pipeline; Efeito do processo de soldagem sobre a microestrutura e a microdureza de juntas soldadas de aco API 5L X80 usado em tubulacoes para transporte de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Alves, R.T.P.; Albuquerque, S.F. [Universidade Federal de Campina Grande (UFCG), PB (Brazil); Maciel, T.M.; Almeida, D.M.; Santos, M.A.

    2008-07-01

    This study had as objective to evaluate the microstructure and microhardness of API 5L X80 steel welded joints, used for pipelines to transport oil and gas, using the Shield Metal Arc Welding process with pre- heating temperature of 200 deg C and 400 deg C and the AWS E8010G electrode as filler metal. For this, besides the microhardness of the welded joint, the weld metals percentiles of micro-constituents and of columnar and regenerated grains and the medium size and extension of the heat affected zone were evaluated. The percentage of acicular ferrite in weld metal ranged from 13% to 33% which generated values of microhardness from 114 HV to 309 HV. (author)

  6. Behavior in fatigue of longitudinal welded joints of pipeline API 5L X-70 steel at constant and variable amplitude; Comportamento em fadiga de juntas soldadas longitudinais de aco para dutos API 5L X-70 em amplitude constante e variavel

    Energy Technology Data Exchange (ETDEWEB)

    Beltrao, Marcelo A.N.; Reis, Felippe T.C.; Castrodeza, Enrique M.; Bastian, Fernando L. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia

    2005-07-01

    The fatigue behavior of base metal, weld metal and heat affected zone of longitudinal welded joints of API 5L X-70 pipeline steel used in oil and gas transportation were studied. Fatigue tests were carried under load control for R = 0.1 and 0.5 with constant and variable amplitude, in this case with application of alternate tensile overloads (75 and 100% of maximum load) at 0,5 mm crack growth intervals. The fatigue tests results were obtained by a vs. N and da/dN vs. {delta}K curves. It can be inferred from a vs. N curves that the specimens submitted to R = 0.5 showed smaller fatigue lives than the ones under R = 0.1, with application of overloads or not. The da/dN vs. {delta}K curves exhibited the highest propagation rates for welded joints submitted to R = 0.5. The overloads promoted delay on crack growth at the three regions of the welded joints, the effect being more pronounced for R 0.1. However, for both R values, the effect of crack propagation delay with the overloads decreased with the increase of {delta}K value. (author)

  7. Induction bending of API 5L X80 pipes; Curvamento a quente de tubos API 5L X80

    Energy Technology Data Exchange (ETDEWEB)

    Batista, Gilmar Z. [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)]. E-mail: g.zacca@petrobras.com.br; Naschpitz, Leonardo [Primus Processamento de Tubos S.A. - PROTUBO, Campo Grande, RJ (Brazil)]. E-mail: naschpitz@protubo.com.br

    2005-07-01

    The present work is a part of an extensive program to make possible the application of API 5L X80 in pipeline construction in Brazil. At this stage, the effect of the induction bending process on the microstructure and mechanical properties of the pipe is presented. For this study an API 5L X80 pipe, made by the UOE process, is used. The pipe was manufactured from a plate produced by thermo-mechanically controlled rolling without accelerated cooling. The pipe bending was carried out applying a local induction heating following by water quenching. The bend section, outer and inner curvature regions, of the pipe bend were evaluated and compared with the original pipe. The longitudinal weld and transition zones were not evaluated at this stage. Dimensional analyses, microstructural evaluation, Charpy-V impact tests, and tensile tests were performed. A significant microstructural change was verified in the bend area. The yield strength of the pipe bend was found to be lower than original pipe and standard requirements. Other tensile properties and impact properties are compatible with API 5L X80 requirements. (author)

  8. Fragilización por Hidrógeno de los Aceros API 5L X60 y API 5L X80 Hydrogen Embrittlement of API 5L X60 and API 5L X80 Steels

    OpenAIRE

    Bruno A Araújo; Jorge A Palma; Vilar, Eudésio O.; Silva, Antonio A.

    2011-01-01

    Se ha determinado el comportamiento mecánico de los aceros API 5L grados X60 y X80, fragilizados por hidrógeno ambiental y por hidrógeno interno. El potencial y la densidad de corriente para la generación del hidrógeno fueron determinados por polarización potenciodinámica, y el tiempo de saturación estimado a partir de datos de difusividad y solubilidad obtenidos en ensayos de permeación con una célula electroquímica. La carga de hidrógeno para reproducir las condiciones de la fragilización p...

  9. Bauschinger effect on API 5L B and X56 steel plates under repeating bending load

    Science.gov (United States)

    Chandra, Icho Y.; Korda, Akhmad A.

    2017-01-01

    During steel pipe fabrication, hot rolled coil steel will undergo coiling and uncoiling process, where the steel plate is bent repeatedly. When cyclic loading is imposed on steel, tensile and compressive stress will occur in it resulting in softening caused by Bauschinger effect. This research is focused on Bauschinger effect and cyclic loading during coiling and uncoiling process on API 5L B and API 5L X56 steel. Both types of steel were given repeated bend loading with variation in loading cycle and the curvature radius. The steel's response was then observed by using tensile testing, microhardness testing, and microstructure observation. A decrease in yield strength is observed during lower cycles and on smaller radii. After higher loading cycle, the yield strength of the steel was increased. Microhardness testing also reported similar results on the subsurface part of the steel where loading is at its highest.

  10. Construction and assembly of pipelines using API 5L grade X80 tubes - considerations to be observed with high-strength steels; Construcao e montagem de dutos terrestres utilizando tubos API 5L Gr. X80

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Ailton C. de; Rabello, Jose Mauricio B. [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)

    2005-07-01

    The use of line pipes API 5L Grade X80, at the point of view of designer allows: reduction of wall thickness and pipe weight or increase of design pressure. In the pipeline construction point of view, the use of line pipes API 5L Grade X80 provide some advantages, however some difficulties must be expected in several stages of the construction and assembly. The implications in cost, productivity, inspection and integrity, with the application of these high resistance steels, complying with PETROBRAS Standard N - 464 Construcao, Montagem e Condicionamento de Dutos Terrestres (Rev. H - 2004 Dec) and the experience consolidated in pipelines construction abroad were presented. At the design stage, a comparison between pipelines designed using API 5L-X70 and API 5L-X80 was carried out approaching the aspects of variation of thickness, pressure design and design factor. An evaluation of the expected gains when choosing API 5L Grade X80 steels were done, regarding reduction of costs and pipe weight. Regarding API 5L-X80 pipe fabrication, the obtained results were reported, proving that this aspect was already overcome, showing the viability of its production in Brazil. Difficulties were detected regarding construction and assembly stage and showing the need of revision of PETROBRAS standard N-464. (author)

  11. Determination of acoustoelastic constant in API-5L-X70 welded pipes; Determinacao da constante acustoelastica de tubos API-5L-X70 com solda

    Energy Technology Data Exchange (ETDEWEB)

    Bittencourt, Marcelo de Siqueira Queiroz; Fonseca, Manoel Antonio da Costa [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Oliveira, Carlos Henrique Francisco de [PETROBRAS S.A., Rio de de Janeiro, RJ (Brazil)

    2008-07-01

    To preserve the environment and avoid financial losses resulting from leaks in the transport of natural oil and gas the industry is particularly concerned to ensure the structural integrity of their pipelines. Grounded pipes may be exposed to damages due to unstable soil movement, and in extreme cases, to failure. Then, techniques to assess stress in pipelines are of central importance. The ultrasonic birefringence technique is an efficient method to measure residual stress. It is based on the changing velocity of the shear wave when it crosses a material under stress; this phenomenon is known as acoustoelasticity. The knowledge of the parameter called acoustoelastic constant is necessary to measure stress using this technique. By the mechanical conformation in the manufacture of welded tubes, from rolled plates, different anisotropy degrees are showed around the cross section of these tubes. In this paper it was studied the acoustoelastic constant for the API-5L-X70 steel pipes, with different thicknesses, diameters and manufacturing processes so as to be able to use this technique to stress measurement in pipelines. (author)

  12. Development of high strength pipes grade API 5L X70 PSL2 offshore by the HFIW Process (High Frequency Induction Welding)

    Energy Technology Data Exchange (ETDEWEB)

    Cordeiro, Wilson R.; Melo, Luis C.R.; Gomes, Igor O.; Boni, Luiz P.; Sanctis, Marco A.M. di [Apolo Tubulars, Lorena, SP (Brazil)

    2009-07-01

    High strength pipes can be manufactured with excellency in dimensional tolerances, rapidity and efficiency through HFIW process (High Frequency Induction Welding). API 5L 6 5/8 x 0,374 in pipes were made of thin, hot rolled and coiled plates with dimensional 9,300 x 1.040,0 x 140.000 mm. Mechanical tests, chemical and microstructural analysis were performed. For the microstructural analysis, ferrite and perlitic structure were detected. All the results obtained are according to API 5L, 44{sup th} edition, 2008 for the grade API 5L X70 PSL2 Offshore. (author)

  13. API 5L X80 PSL2 pipes manufactured by ERW process: TenarisConfab and USIMINAS development; Tubos API 5L X80 PSL2 processados por ERW: desenvolvimento TenarisConfab-Usiminas

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Ronaldo C.; Roza, Juliana E.; Kojima, Sergio S. [TenarisConfab, Pindamonhangaba, SP (Brazil); Miranda, Lucio S.; Turani, Leonardo O. [USIMINAS, Ipatinga, MG (Brazil)

    2005-07-01

    The utilization of high strength steels can decrease, both, installation and operation costs of a new pipeline, since the increase of mechanical resistance can reduce the pipe wall thickness and, consequently, the weight of the pipe. For this purpose, the manufacture of electric resistance welding (ERW) pipes is recent, and it comes as an opportunity for gas pipelines, mainly onshore projects. As a function of their larger productivity, ERW pipes are more economically attractive than those produced by seamless and SAW processes. TenarisConfab and USIMINAS, through studies and investments in manufacturing processes, have been working to increase their skills, in order to supply ERW pipes in API 5L X80 PSL2 steel. The steel elaborated by USIMINAS is low C, high Mn, Nb, V and Ti microalloyed, and with additions of Cr and Mo, aiming to produce hot rolled coils with acicular microstructure, achieved as a combination between its chemical composition and higher cooling rates after hot rolling. The results in {phi}18''(457 mm) x 0,469''(11,91 mm) pipes are presented in this article. High toughness values reached at very low temperatures suggest that ERW API 5L X80 PSL2 pipes developed by TenarisConfab and USIMINAS may be an excellent option of choice for onshore projects that request pipes in diameters up to 20''(508 mm) and thickness up to 0,500'' (12,7 mm). (author)

  14. Corrosion resistance of ERW (Electric Resistance Welded) seam welds as compared to metal base in API 5L steel pipes

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Velasquez, Jorge L.; Godinez Salcedo, Jesus G.; Lopez Fajardo, Pedro [Instituto Politecnico Nacional (IPN), Mexico D.F. (Mexico). Escuela Superior de Ingenieria Quimica e Industrias Extractivas (ESIQIE). Dept. de Ingenieria Metalurgica

    2009-07-01

    The corrosion resistance of ERW seam welds and the base metal in API 5L X70 steel pipes was evaluated by Tafel tests. The procedure was according to ASTM G3 standard. The study was completed with metallographic and chemical characterization of the tested zones, that is, the welded zone and the base metal away of the weld. All tests were made on the internal surface of the pipe in order to assess the internal corrosion of an in-service pipeline made of the API 5L X70 steel. The test solution was acid brine prepared according to NACE Publications 1D182 and 1D196. The results showed that the ERW seam weld corrodes as much as three times faster than the base material. This behavior is attributed to a more heterogeneous microstructure with higher internal energy in the ERW seam weld zone, as compared to the base metal, which is basically a ferrite pearlite microstructure in a normalized condition. This result also indicates that pipeline segments made of ERW steel pipe where the seam weld is located near or at the bottom of the pipe are prone to a highly localized attack that may form channels of metal loss if there is water accumulation at the bottom of the pipeline. (author)

  15. API SPEC 5L会标认证审核企业需做的主要准备工作%The Main Preparatory Work for Enterprise API SPEC 5L Monogram Certification

    Institute of Scientific and Technical Information of China (English)

    方晓东

    2009-01-01

    随着TSG Z0004标准实施和API SPEC 5L以及API SPEC Q1标准的换版,当前申请API SPEC 5L会标使用许可证的企业应及时做好各项准备工作,接受审核组的审核.审核前需做的准备工作主要包括:①及时与审核员充分沟通;②各种审核文件的整理和完善;③维护和保养生产现场设备,完善各岗位现场作业指导文件,确保现场各种记录完整清楚.%Along TSG Z0004 standard implement and renewal edition of API SPEC 5L and API SPEC Q1,enterprises should make all preparations for applying API SPEC 5L monogram use permit right to receive auditor check.The main preparatory work include:①to completely communicate with auditor in time;②to file and improve all kinds of documents which will be checked;③to maintain equipments in production site,improve each job-site guidance document,and insure all kinds of records integrity and clarity. Key words:certification audit;API SPEC 5L;API SPEC Q1;monogram use permit

  16. Comportamiento de Laminaciones Coplanares y No Coplanares en Acero API5L X52 Simuladas mediante Elementos Finitos Behavior of Coplanar and Non-Coplanar Laminations in API5L X52 Steel Simulated by Finite Elements

    Directory of Open Access Journals (Sweden)

    Alberto Servin

    2009-01-01

    Full Text Available Se determina el comportamiento mecánico de laminaciones simples y escalonadas modeladas por elementos finitos en condiciones no lineales de esfuerzo-deformación, para comprender la secuencia de la interacción de los campos de esfuerzos en los extremos de la grietas. Se estableció una matriz de simulación variando la extensión circunferencial de las laminaciones, con espesor del tubo de 610mm de diámetro, con separación vertical y horizontal para el caso de laminaciones escalonadas. Se determino la función de distribución de esfuerzos en la región de las laminaciones en función de la presión en la grieta y en el tubo. Se estableció la presión que origina alcanzar la cedencia y resistencia ultima del material API5L X52, determinando que las laminaciones simples cuya extensión circunferencial va de 24 a 127 mm, soportan 50% más de presión de cedencia que las laminaciones escalonadas, y el 100% más de presión máxima permisible. Basados en la graficas presión versus radio de las laminaciones se concluye que las laminaciones no coplanares representan mayor riesgo de agrietamiento en una tubería.The mechanical behavior of the coplanar and non coplanar laminations by finite element using non lineal material conditions was determined, with the purpose of knowing the interaction sequence of the stress fields in the crack tips. The analysis includes a matrix simulation considering the circumferential length lamination, wall thickness of the pipe 610mm diameter, with vertical and horizontal distance for non coplanar laminations. The stress distribution in the laminations regions as a function of the internal pressures in the cracks and pipe were determined. The simulations done allowed defining the yield and the strength pressure that reach the mechanical properties of the API 5L X52. It was found that coplanar laminations with 24 at 127mm of circumferential length resist 50% more yield pressure than the non coplanar laminations and

  17. Microbial Corrosion of API 5L X-70 Carbon Steel by ATCC 7757 and Consortium of Sulfate-Reducing Bacteria

    Directory of Open Access Journals (Sweden)

    Arman Abdullah

    2014-01-01

    Full Text Available Various cases of accidents involving microbiology influenced corrosion (MIC were reported by the oil and gas industry. Sulfate reducing bacteria (SRB have always been linked to MIC mechanisms as one of the major causes of localized corrosion problems. In this study, SRB colonies were isolated from the soil in suspected areas near the natural gas transmission pipeline in Malaysia. The effects of ATCC 7757 and consortium of isolated SRB upon corrosion on API 5L X-70 carbon steel coupon were investigated using a weight loss method, an open circuit potential method (OCP, and a potentiodynamic polarization curves method in anaerobic conditions. Scanning electron microscopy (SEM and energy dispersive X-ray spectroscopy (EDS were then used to determine the corrosion morphology in verifying the SRB activity and corrosion products formation. Results from the study show that the corrosion rate (CR of weight loss method for the isolated SRB is recorded as 0.2017 mm/yr compared to 0.2530 mm/yr for ATCC 7757. The Tafel plot recorded the corrosion rate of 0.3290 mm/yr for Sg. Ular SRB and 0.2500 mm/yr for Desulfovibrio vulgaris. The results showed that the consortia of isolated SRB were of comparable effects and features with the single ATCC 7757 strain.

  18. Corrosion rate of API 5L Gr. X60 multipurpose steel pipeline under combined effect of water and crude oil

    Science.gov (United States)

    Miao, Jian; Wang, Qiang

    2016-09-01

    Multipurpose pipeline is often seriously corroded during its service life, and the phenomenon is more prominent once the transportation medium is changed. Electrochemical polarization curves and impedance spectroscopy of the API 5L Gr. X60 steel pipeline's corrosion process in sedimentary water with different ion types and their concentrations have been studied in this work. The results showed that the corrosion rates were found to be 0.00418 and 0.00232 mm/a for pure water and crude oil, respectively. However, for the mixtures of water and crude oil (with water content increased from 0.2 vol% to 10 vol%), the corrosion rate increased consistently and reached a maximum value of 0.15557 mm/a for 10 vol% water in crude oil. The effect of the concentration of various ions, namely, chloride, bicarbonate and sulfate in (oil/water) mixtures on the corrosion rate was characterized by weight-loss method. The results showed that with increasing the ions' concentrations, the corresponding exchange current density increased significantly. The results were further supported by the observations of corrosion morphology using scanning electron microscopy and are helpful in devising guidelines which would help in reducing corrosion in multipurpose transport pipelines involving a change of transported medium during their service life.

  19. Influencia del precalentamiento en las propiedades de uniones soldadas de acero API 5L-X80 soldadas con alambre tubular autoprotegido

    Directory of Open Access Journals (Sweden)

    Cooper, R.

    2004-08-01

    Full Text Available The present work refers to the characterization of API 5L-X80 pipeline joints welded with self-shielded flux cored wire. This process was evaluated under preheating conditions, with an uniform and steady heat input. All joints were welded in flat position (IG, with the pipe turning and the torch still. Tube dimensions were 762 mm in external diameter and 16 mm in thickness. Welds were applied on single V-groove, with six weld beads, along with three levels of preheating temperatures (room temperature, 100 °C, 160 °C. These temperatures were maintained as interpass temperature. The filler metal E71T8-K6 with mechanical properties different from parent metal was used in undermatched conditions. The weld characterization is presented according to the mechanical test results of tensile strength, hardness and impact test. The mechanical tests were conducted according to API 1104, AWS and ASTM standards. API 1104 and API 51 were used as screening criteria. According to the results obtained, it was possible to remark that it is appropriate to weld API 5L-X80 steel ducts with Self-shielded Flux Cored wires, in conformance to the API standards and no preheat temperature is necessary.

    Este trabajo presenta un estudio sobre la caracterización de las propiedades mecánicas de uniones soldadas con alambre tubular autoprotegido, de acero API 5L-X80. En este sentido, se evalúa la influencia de la temperatura de precalentamiento, manteniendo el aporte de calor uniforme. Todas las uniones soldadas se realizaron en la posición plana (IG, con la antorcha fíja y la tubería girando. Se utilizó un tubo de 762 mm de diámetro exterior y 16 mm de espesor. Las soldaduras se aplicaron sobre una unión de tope con bisel en V simple, utilizando 6 pasadas, con tres niveles de temperatura de precalentamiento (ambiente, 100 °C y 160 °C. Estas temperaturas se mantuvieron como temperatura entre pasadas. El metal de aporte, del tipo E71T8-K6, se utilizó en la

  20. Caracterización de aceros API-5L por medio de la medición de ruido magnético Barkhausen

    OpenAIRE

    P. Martínez-Ortiz; J. H Espina-Hernández; Hallen, J. M.; R. Linares y Miranda

    2010-01-01

    En este trabajo se presenta la caracterización de aceros API- 5L (grado X52, X56, X60) a través de ruido magnético Barkhausen. Un campo magnético sinusoidal de 10 Hz de frecuencia e intensidad de 5.2 kA/m se aplicó a las muestras de acero API-5L. Se demostró que cada acero genera un señal de ruido magnético Barkhausen propia que depende de su composición química y microestructura. Se comprobó la anisotropía magnética de los aceros a través de la señal de ruido magnético Barkhausen. Se proces...

  1. The Effect of Temperature and Acid Gas Loading on Corrosion Behavior of API 5L X52 Carbon Steel in Amine Unit

    Science.gov (United States)

    Javidi, M.; Lalehparvar, M. M.; Ghassemi, A.

    2016-05-01

    The effect of temperature and H2S concentration on amine corrosion of API 5L X52 carbon steel in a CO2-saturated 25 wt.% diethanolamine solution was investigated via electrochemical techniques. It was found that increase in temperature from 25 to 80 °C resulted in severe increase in corrosion rate from 0.88 to 16.24 mpy due to increase in degradation rate of amine. Also, it was concluded that increase in H2S concentration led to increase in corrosion rate because of formation of more heat stable amine salts. The effect of temperature on corrosion rate was more significant than acid gas loading.

  2. Residual stress evaluation by X-Ray diffraction and hole-drilling in an API 5L X70 steel pipe bent by hot induction

    Energy Technology Data Exchange (ETDEWEB)

    Ceglias, Rodrigo Braga; Alves, Juciane Maria; Botelho, Ramon Alves; Baeta Junior, Eustaquio de Souza; Santos, Igor Cuzzuol dos; Moraes, Nicki Robbers Darciano Cajueiro de; Oliveira, Rebeca Vieira de; Diniz, Saulo Brinco; Brandao, Luiz Paulo, E-mail: brandao@ime.eb.br [Instituto Militar de Engenharia (IME), Rio de Janeiro, RJ (Brazil). Secao de Engenharia Mecanica e de Materiais

    2016-09-15

    The API 5L X70 steel is used in high-pressure gas transmission pipelines. Because of this, knowledge of presence of residual stress and their magnitude is important to assess the material integrity in service. For the pipeline manufacturing, tubes need to be curved which is often made using the hot induction bending process. This process can introduce different residual stress depending of tube position. For this research, in order to evaluate the residual stress, was used an API 5L X70 tube that was previously curved by hot induction process. Samples were taken from the extrados, intrados, neutral line and straight section of the curved tube. Residual stresses were studied by two conventional methods: X-Ray Diffraction (XRD) and Hole-Drilling, which are destructive and non-destructive methods, respectively, in order to assess their qualitative responses. Each of these methods presents particular methodologies in sample preparation and material analysis, but also they differ in factors such time consumption and cost of the analysis. The qualitative responses obtained by the two different methods were comparable and satisfactory and pointed out the existence of a compressive residual stress state in steel pipe. (author)

  3. APIs

    CERN Document Server

    Jacobson, Daniel; Woods, Dan

    2011-01-01

    Programmers used to be the only people excited about APIs, but now a growing number of companies see them as a hot new product channel. This concise guide describes the tremendous business potential of APIs, and demonstrates how you can use them to provide valuable services to clients, partners, or the public via the Internet. You'll learn all the steps necessary for building a cohesive API business strategy from experts in the trenches. Facebook and Twitter APIs continue to be extremely successful, and many other companies find that API demand greatly exceeds website traffic. This book offe

  4. Estudio del daño por hidrógeno en uniones soldadas de un acero API 5L X52

    Directory of Open Access Journals (Sweden)

    Réquiz, R.

    2008-04-01

    Full Text Available The main objective of the present investigation was to study the susceptibility to hydrogen damage on a type API 5L X52 steel welded by electrical resistance. Several techniques, such as hydrogen permeation and cathodic charging were used. The metallic material was characterized using SEM and TEM. The base metal microstructure was very similar to that one corresponding to the welded area. This microstructure was mainly comprised by ferrite and perlite, differing only in the grain size. Therefore, the susceptibility to hydrogen damage was similar in both cases. It is worth mentioning that the welded area has very small dimensions. Indeed, the fusion zone is only 5 mm wide while the heat affected zone is 1 mm wide. The hydrogen damage observed was mainly in the form of blisters, which were associated to the presence of aluminum rich inclusions. Also, it was noticed partial inclusion dissolution and some matrix attack adjacent to the inclusions.

    En la presente investigación se estudió la susceptibilidad al daño por hidrógeno en un acero API 5L X52 soldado por resistencia eléctrica. Para ello, se emplearon técnicas de permeación y de carga catódica de hidrógeno. El material fue caracterizado mediante microscopía electrónica de barrido y de transmisión. La susceptibilidad al daño por hidrógeno fue similar tanto en el metal base como en el cordón de soldadura. Este comportamiento se vincula a la microestructura homogénea constituida por perlita y ferrita, que presentan ambos, aun cuando existen diferencias en el tamaño de grano. El cordón de soldadura tenía dimensiones muy pequeñas, donde la zona de fusión y la afectada por el calor poseen espesores de 5 mm y 1 mm, respectivamente. El daño inducido por hidrógeno se presentó en forma de ampollas en la superficie del material, asociado, principalmente, a la presencia de inclusiones globulares y semiglobulares ricas en aluminio. Además, se manifestó una disoluci

  5. Numerical Evaluation of Temperature Field and Residual Stresses in an API 5L X80 Steel Welded Joint Using the Finite Element Method

    Directory of Open Access Journals (Sweden)

    Jailson A. Da Nóbrega

    2016-01-01

    Full Text Available Metallic materials undergo many metallurgical changes when subjected to welding thermal cycles, and these changes have a considerable influence on the thermo-mechanical properties of welded structures. One method for evaluating the welding thermal cycle variables, while still in the project phase, would be simulation using computational methods. This paper presents an evaluation of the temperature field and residual stresses in a multipass weld of API 5L X80 steel, which is extensively used in oil and gas industry, using the Finite Element Method (FEM. In the simulation, the following complex phenomena were considered: the variation in physical and mechanical properties of the material as a function of the temperature, welding speed and convection and radiation mechanisms. Additionally, in order to characterize a multipass weld using the Gas Tungsten Arc Welding process for the root pass and the Shielded Metal Arc Welding process for the filling passes, the analytical heat source proposed by Goldak and Chakravarti was used. In addition, we were able to analyze the influence of the mesh refinement in the simulation results. The findings indicated a significant variation of about 50% in the peak temperature values. Furthermore, changes were observed in terms of the level and profile of the welded joint residual stresses when more than one welding pass was considered.

  6. Pengaruh Variasi Goresan Lapis Lindung Dan Variasi pH Tanah Terhadap Arus Proteksi Sistem Impressed Current Cathodic Protection (ICCP Pada Pipa API 5L Grade B

    Directory of Open Access Journals (Sweden)

    Trendy Leo Pratama

    2015-03-01

    Full Text Available Korosi merupakan penyebab utama terjadinya kegagalan material pipeline akibat berinteraksi secara langsung dengan lingkungan. Pemberian lapis lindung yang selama ini menjadi pilihan utama untuk mengontrol korosi tidak selamanya bisa diandalkan. Hal ini disebabkan kemungkinan terjadinya kerusakan lapis lindung selama proses shipping atau instalasi sangatlah besar. Oleh karena itu sering kali pemberian lapis lindung dikolaborasikan dengan perlindungan sistem proteksi katodik, khususnya sistem arus paksa (ICCP. Penelitian ini bertujuan mempelajari pengaruh luas goresan lapis lindung dan pH tanah terhadap arus proteksi sistem proteksi katodik arus paksa (ICCP. Goresan yang diberikan berbentuk lingkaran dan persegi panjang dengan luas 10 mm2, 50 mm2, 100 mm2, 150 mm2, 250 mm2, dan 500 mm2. Spesimen tanpa goresan dan tanpa lapis lindung digunakan sebagai pembanding. Sedangkan untuk variasi pH tanahnya adalah pH 3 (asam, pH 7 (netral, dan pH 11 (basa. Pipa API 5L grade B digunakan sebagai katoda dan grafit sebagai anoda, serta rectifier sebagai penyearah arus (DC. Arus proteksi ICCP diatur hingga mencapai nilai potensial proteksi -850 mV vs elektroda Cu/CuSO4. Setelah dilakukan pengkondisian awal selama 8 hari dan 7 hari pengukuran arus, didapatkan hasil bahwa semakin semakin besar goresan lapis lindung maka semakin besar arus proteksi yang dibutuhkan dalam kondisi pH tanah yang sama. Sedangkan dalam kondisi luas goresan yang sama, kebutuhan arus proteksi meningkat seiring dengan semakin rendahnya pH tanah (semakin asam

  7. Influencia del acabado superficial en la permeación de hidrógeno del acero API 5L-X52

    Directory of Open Access Journals (Sweden)

    Réquiz, R.

    2004-02-01

    Full Text Available The influence of surface roughness on the corrosion and hydrogen permeation behaviour was evaluated on a type API 5L-X52 steel in deaerated 0.1 M Na2SO4 at pH=2. Potentiodynamic polarization curves were employed to determine the electrochemical behaviour of the steel, while the Devanathan-Stachurski technique was used to estimate the hydrogen permeation rate. Additionally, the surface roughness profiles were obtained in order to correlate the changes in the hydrogen permeation rate with different metal surface finishings. The obtained results clearly demonstrate that when the roughness parameters have larger values, the cathodic current of hydrogen evolution increases while the hydrogen entry rate decreases. This effect can be attributed to the microstructural defects induced at the steel surface, such as dislocations, which increase the catalytic activity of the atomic hydrogen favouring its recombination. Also, these defects could allow the atomic hydrogen to remain adsorbed on the steel surface. Both effects could hinder the hydrogen diffusion into the metal since the possibility for this atom of becoming absorbed has been reduced

    En el presente trabajo de investigación se evalúa la influencia del acabado superficial, tanto en la corrosión generalizada como en la permeación de hidrógeno del acero API 5L-X52 en solución de Na2SO4 0,1M, a pH=2. El comportamiento electroquímico del acero se estudió mediante las curvas de polarización potenciodinámicas, mientras que la susceptibilidad al daño por hidrógeno se evaluó mediante la técnica de permeación de Devanathan- Stachurski. Además, se determinaron los parámetros de rugosidad a las muestras de acero con diferentes acabados superficiales, para luego correlacionarlos con los cambios en las corrientes de permeación de hidrógeno. Los resultados obtenidos demuestran, claramente, que, cuando la rugosidad superficial del acero

  8. Technological impact in steels degree API 5L X-70 for the manufacture of resistant ducts of 36 inches of diameter to the bitter gas; Impacto tecnologico de aceros grado API 5L X-70 para la fabricacion de ductos de 36 pulgadas de diametro resistentes al gas amargo

    Energy Technology Data Exchange (ETDEWEB)

    Aramburo Perez, G.; Garcia Galan, S.; Perez Campos, R.; Juarez Islas, J.A. [Facultad de Quimica, UNAM, Mexico, D.F. (Mexico)

    2004-03-01

    , cumplen con las propiedades del acero grado API 5L X-70, las cuales son requeridas en los tubos de acero por la industria petrolera.

  9. Avaliação da resistência à corrosão-fadiga no aço API 5L X60 em meio aquoso salino

    OpenAIRE

    Juciele Leyter Zilio

    2013-01-01

    As condições para a exploração do petróleo em campos do pré-sal são mais severas que as convencionais no que se refere à pressão, temperatura, concentração de CO2 e H2S. Nesse contexto, os dutos responsáveis pela transferência do petróleo do fundo do mar até os tanques de armazenamento na superfície representam uma importância fundamental no processo de produção do petróleo. Dessa forma, o objetivo deste trabalho foi estudar o comportamento em corrosão-fadiga de juntas soldadas do aço API 5L ...

  10. Mechanical properties of API X80 steel pipe joints welded by Flux Core Arc Weld Process; Propriedades mecanicas de juntas de tubos de aco API X80 soldadas com arame tubulares

    Energy Technology Data Exchange (ETDEWEB)

    Ordonez, Robert E. Cooper; Silva, Jose Hilton F.; Trevisan, Roseana E. [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Mecanica. Dept. de Engenharia de Fabricacao

    2003-07-01

    Flux Core Arc Welding processes (FCAW) are beginning to be applied in pipeline welds, however, very limited experimental data regarding mechanical properties of pipeline weld joints with these processes are available in the literature. In this paper, the effects of preheat temperature and type of FCAW on mechanical properties (microhardness and tensile strength) of API X80 weld joint steel are presented. FCAW processes with gas protection and self-shielded were used. Multipasses welding were applied in 30'' diameter and 0,625'' thickness tubes. Influence factors were: FCAW type and preheat temperature. Acceptance criteria of welded joints were evaluated by API 1104 standard for tensile strength test and ASTM E384-99 for microhardness test. The results obtained showed that FCAW type and preheat temperature have no influence on mechanical properties of API X80 joint steel. (author)

  11. Study of the sulphide stress corrosion cracking (SSCC) resistance of API SL GR B and X60 pipeline steels. Evaluacion de la resistencia al agrietamiento por corrosion-tension en medios sulfhidricos (SSCC) de aceros para tuberias Calidad API 5L Grados B y X60

    Energy Technology Data Exchange (ETDEWEB)

    Bao-Iturbe, C. (Babcock and Wilcox Espaola. S.A. Bilbao (Spain)); Gutierrez de Saiz-Solabarria (Univ. Pais Vasco. Departamento Ingenieria Metalurgica y Control de Materiales. Bilbao (Spain))

    1993-01-01

    A study of the sulphide stress corrosion cracking resistance at room temperature of API 5L Cr B and X60 pipeline steels has been carried out. The theoretical mechanisms in order to explain these phenomena and several operational failures of pipeline steel due to SSCC have been reviewed and the National Association of Corrosion Engineers (NACE) standard concerning SSCC has been described. The main factors of influence of the SSCC have been analysed, results are presented and conclusions are elaborated. (Author) 32 ref.

  12. Avaliação da microestrutura e propriedades mecânicas de metais de solda obtidos por processos de soldagem manual e automatizado utilizado na soldagem de aço API 5L X80 Evaluation of microstructure and mechanical properties of weld metals obtained by manual and automated welding process used in the welding of API 5L X80 steel

    Directory of Open Access Journals (Sweden)

    Siderley Fernandes Albuquerque

    2011-12-01

    Full Text Available O objetivo do trabalho foi avaliar as características da zona termicamente afetada (ZTA e a microestrutura e propriedades mecânicas de metais de solda de juntas soldadas do aço API 5L X80, obtidos para quatro diferentes procedimentos de soldagem utilizando processos manuais e automatizados. Para isto, chapas do referido aço foram soldadas por processo manual ao Arco Elétrico com Eletrodo Revestido (SMAW, utilizando 473 e 673 K como temperaturas de interpasses e o eletrodo celulósico AWS E8010-G como consumível; por processo ao Arco Elétrico com Arame Tubular (FCAW robotizado, utilizando o arame AWS E71T- 1C como metal de adição e argônio com 25%CO2 como gás de proteção; por processo a Arco Elétrico com Eletrodo de Tungstênio (GTAW mecanizado na raiz da solda, usando o arame ER70S-3 e argônio como gás de proteção. As análises microestruturais foram relacionadas com os resultados de ensaios de impacto Charpy nos metais de solda e com os perfis de microdureza Vickers ao longo da junta soldada. Os resultados indicaram maiores percentuais de Ferrita Acicular e maiores valores de resistência ao impacto nos metais de solda e uma menor extensão e granulometria da ZTA, associado ao procedimento de soldagem utilizando processo automatizado com maior velocidade de soldagem.The objective of this work was to evaluate the heat affected zone characteristics and weld metals microstructure and mechanical properties of API 5L X80 steel welded joints, obtained for four different welding procedures using manual and automated processes. For this, plates of this steel were welded by manual Shielded Metal Arc Welding (SMAW process with interpasses temperatures of 473 e 673 K, and using AWS E8010-G electrode as filler metals; robotized Flux Cored Arc Welding (FCAW process, using AWS E71T-1C wire and Ar25%CO2 as consumable and mechanized Gas Tungsten Arc Welding (GTAW process, for the root pass using AWS ER70S-3 and Ar as consumable .The

  13. DESGASTE POR ABRASIÓN DEL ACERO API 5L X65 REVESTIDO CON NIOBIO POR ASPERSIÓN TÉRMICA A PLASMA Y CON INCONEL 625 POR SOLDADURA

    Directory of Open Access Journals (Sweden)

    JOSE MATOS

    2012-01-01

    Full Text Available El objetivo de este trabajo fue evaluar y caracterizar el comportamiento mecánico en desgaste del acero API 5L X65, revestido con niobio en comparación al desempeño del revestimiento de la aleación de inconel 625 empleados en la industria de petróleo y gas. El revestimiento de niobio fue obtenido por el proceso de aspersión térmica a plasma de arco no transferido y el revestimiento inconel 625 por soldadura con electrodo revestido. La resistencia al desgaste por abrasión fue evaluada según la norma Petrobras N-2568, en un tribómetro CTER, la rugosidad y el volumen de material desgastado se determinó a través de perfilometría y la dureza de los revestimientos por microscopia Vickers. Los revestimientos obtenidos fueron caracterizados respecto a su morfología por microscopia electrónica de barrido (MEB y microscopía óptica (MO. La mayor dureza del revestimiento con niobio obtenido puede haber contribuido a reducir la tasa de desgaste en comparación con el revestimiento de inconel 625.

  14. Pioneer ACO PUF

    Data.gov (United States)

    U.S. Department of Health & Human Services — Pioneer ACO PUF - To address the increasing number of requests for Pioneer ACO data, the Centers for Medicare and Medicaid Services (CMS) has created a standard...

  15. Evaluación de la susceptibilidad a la corrosión por picado del acero API 5L x42 expuesto a un ambiente con cloruros y CO2 mediante la técnica de ruido electroquímico

    Directory of Open Access Journals (Sweden)

    Rodríguez-Vanegas, N.

    2011-10-01

    Full Text Available The concentration of chloride ions and the partial pressure of CO2 play an important role in the degradation of low-carbon steels used for the construction of pipelines in oil and gas industry. In order to evaluate the susceptibility of carbon steel API 5L X42 to pitting corrosion electrochemical noise and linear polarization resistance measurements were carried out in aqueous solutions containing chloride ions and CO2. The concentration of chloride ions was varied between, 10000 and 18000 ppm, and the CO2 partial pressure between 10 psi and 18 psi. Experimental results pointed out that the formation of protective layer, consisting mainly of FeCO 3, depends on the partial pressure of CO2 in the system. Nevertheless, the stability of this layer was considerably affected by increasing the concentration of chloride ions causing that localized corrosion has taken place in some areas of the surface of API 5L X42, which were detected by electrochemical noise echnique.La concentración de iones cloruro y la presión parcial de CO2, tienen un papel importante en el proceso de degradación de los aceros de bajo carbono empleados en la construcción de líneas de transporte en industrias petroleras. Con el fin de evaluar la susceptibilidad del acero al carbono API 5L X42 a la corrosión por picado, se realizaron medidas de ruido electroquímico y resistencia a la polarización lineal en soluciones acuosas de iones cloruro con concentraciones entre 10.000 y 18.000 ppm, variando la presión parcial de CO2 entre 10 psi y 18 psi. Los resultados indican que la formación de una capa protectora, constituida principalmente por FeCO3, depende de la presión parcial de CO2 del sistema. No obstante, la estabilidad de dicha capa es afectada significativamente por el aumento de la concentración de iones cloruro, que producen fenómenos de corrosión localizada en algunas áreas de la superficie del acero API 5L X42, los cuales fueron detectados por la técnica de

  16. “Corrosión Inducida por Bacterias Sulfato Reductoras Termófilas de 60°C en la Unión Soldada del Acero API 5L- X60.”

    OpenAIRE

    Romero Romero, Justo Román

    2012-01-01

    En esta investigación se estudio el efecto de la corrosión inducida por Bacterias Sulfato Reductoras (BSR) termófilas a 60oC en el metal base (MB) y la unión soldada (US) del acero API X-60. Las bacterias sulfato reductoras fueron aisladas en el medio sólido postgate con la técnica de estriado, y fueron resembradas hasta obtener la misma morfología. El medio postgate fue seleccionado entre otros medios de cultivo, por el óptimo crecimiento que presento la BSR. Se preparar...

  17. Pioneer ACO Model

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Pioneer ACO Model is designed for health care organizations and providers that are already experienced in coordinating care for patients across care settings. It...

  18. Helioviewer API

    Data.gov (United States)

    National Aeronautics and Space Administration — The Helioviewer Project maintains a set of Public APIs with the goal of improving access to solar and heliospheric datasets to scientists, educators, developers,...

  19. Recalls API

    Data.gov (United States)

    General Services Administration — This Recalls API allows you to tap into a list of (1) drug and food safety recalls from the Food and Drug Administration, Food Safety and Inspection Service, and...

  20. Jobs API

    Data.gov (United States)

    General Services Administration — This Jobs API returns job openings across the federal government and includes all current openings posted on USAJobs.gov that are open to the public and located in...

  1. Drug Interaction API

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Interaction API is a web service for accessing drug-drug interactions. No license is needed to use the Interaction API. Currently, the API uses DrugBank for its...

  2. Feocromocitoma cardíaco

    Directory of Open Access Journals (Sweden)

    Gustavo L. Knop

    2006-01-01

    Full Text Available Los feocromocitomas cardíacos primarios (FCP son sumamente infrecuentes. Hasta el presente son menos de 50 los casos comunicados en el mundo. Presentamos el caso de un tumor intrapericárdico, que resultó ser un feocromocitoma primario, en una mujer de mediana edad, cuyo signo principal fue hipertensión arterial severa (HTAs. Los estudios diagnósticos por imágenes corroboraron la presencia de un tumor intrapericárdico como único hallazgo y los estudios bioquímicos de catecolaminas y sus metabolitos excretados por orina reafirmaron el diagnóstico etiológico. El tumor fue resecado quirúrgicamente sin complicaciones mediante cirugía cardíaca convencional con circulación extracorpórea (CEC y paro cardíaco con cardioplejía. Siete meses después de la operación, la paciente se encuentra asintomática y normotensa.

  3. Study of an API migration for two XML APIs

    NARCIS (Netherlands)

    Bartholomei, T.T.; Czarnecki, K.; Lämmel, R.; Storm, T. van der; Brand, M.G.J. van den; Gasevic, D.; Gray, J.

    2010-01-01

    API migration refers to adapting an application such that its dependence on a given API (the source API) is eliminated in favor of depending on an alternative API (the target API) with the source and target APIs serving the same domain. One may attempt to automate API migration by code transformatio

  4. ACO model should encourage efficient care delivery.

    Science.gov (United States)

    Toussaint, John; Krueger, David; Shortell, Stephen M; Milstein, Arnold; Cutler, David M

    2015-09-01

    The independent Office of the Actuary for CMS certified that the Pioneer ACO model has met the stringent criteria for expansion to a larger population. Significant savings have accrued and quality targets have been met, so the program as a whole appears to be working. Ironically, 13 of the initial 32 enrollees have left. We attribute this to the design of the ACO models which inadequately support efficient care delivery. Using Bellin-ThedaCare Healthcare Partners as an example, we will focus on correctible flaws in four core elements of the ACO payment model: finance spending and targets, attribution, and quality performance.

  5. Analyzing the Change-Proneness of APIs and web APIs

    NARCIS (Netherlands)

    Romano, D.

    2015-01-01

    Analyzing the Change-Proneness of APIs and web APIs APIs and web APIs are used to expose existing business logic and, hence, to ease the reuse of functionalities across multiple software systems. Software systems can use the business logic of legacy systems by binding their APIs and web APIs. With t

  6. Healthcare Finder API

    Data.gov (United States)

    U.S. Department of Health & Human Services — All of the data used on the Finder.HealthCare.gov web application is available through this API. There are multiple collections of data available through the API. 1....

  7. NASA Techport API

    Data.gov (United States)

    National Aeronautics and Space Administration — The NASA TechPort system provides a RESTful web services API to make technology project data available in a machine-readable format. This API can be used to export...

  8. Trasplante cardíaco

    Directory of Open Access Journals (Sweden)

    Dimitri Novitzky

    2007-01-01

    Full Text Available En 1985, en el Tampa General Hospital se inició el Programa de Trasplante Cardíaco. Hasta la fecha se realizaron 815 trasplantes en 795 pacientes, 17 de los cuales fueron sometidos a un retrasplante. La sobrevida actuarial al mes es del 94,5%, al año del 87%, a los 3 años alcanza el 81%, a los 5 años el 74% y a los 10 años el 52%.El Medical Review Board se reúne semanalmente para un seguimiento de la presentación de pacientes y la selección de los candidatos adecuados para el programa. Dentro de las varias Organ Procurement Organizations of the Region, la institución de terapia hormonal para el donante y el receptor está ampliamente aceptada. En el Tampa General Hospital también se le administra levotiroxina al receptor, en la sala de operaciones y en la sala de cuidados intensivos en la situación de un bajo volumen minuto. La mayoría de los trasplantes se efectúan en pacientes con estado 1A o 1B. La inmunosupresión es indicada por especialistas cardiólogos, lo mismo que la realización de la biopsia endomiocárdica con el fin de ajustar la medicación inmunosupresora. En esta revisión se presentan los resultados generales del programa, la selección de pacientes, los procedimientos quirúrgicos, la inmunosupresión y la asistencia que requieren los ventrículos

  9. Android quick APIs reference

    CERN Document Server

    Cinar, Onur

    2015-01-01

    The Android Quick APIs Reference is a condensed code and APIs reference for the new Google Android 5.0 SDK. It presents the essential Android APIs in a well-organized format that can be used as a handy reference. You won't find any technical jargon, bloated samples, drawn out history lessons, or witty stories in this book. What you will find is a software development kit and APIs reference that is concise, to the point and highly accessible. The book is packed with useful information and is a must-have for any mobile or Android app developer or programmer. In the Android Quick APIs Refe

  10. Isolation and characterization of Aconitate hydratase 4 (Aco4) from soybean

    Science.gov (United States)

    Aconitase catalyzes the reversible isomerization of two tricarboxylic acids citrate and isocitrate during the Krebs cycle. Five aconitase genes namely Aco1, Aco2, Aco3, Aco4 and Aco5 have been identified in soybean. Previously, Aco4 was mapped on chromosome 11. The purpose of this investigation was ...

  11. API Global Sourcing Strategies 2010.

    Science.gov (United States)

    Bennett, Shannon

    2010-09-01

    The API Global Sourcing Strategies 2010 Conference, held in Berlin, included topics covering new developments in the field of global sourcing of active pharmaceutical ingredients (APIs). This conference report highlights selected presentations on development in Eastern API markets, specifically India and China, factors influencing changes in global API sourcing, and risk mitigation in API sourcing.

  12. Numerical Study of Hydrogen Trapping: Application to an API 5L X60 Steel

    OpenAIRE

    Patricia Castaño-Rivera; Ramunni, Viviana P.; Pablo Bruzzoni

    2012-01-01

    A numerical finite difference method is developed here to solve the diffusion equation for hydrogen in presence of trapping sites. A feature of our software is that an optimization of diffusion and trapping parameters is achieved via a non linear least squares fit. On the other hand, we have demonstrated that usual electrochemical hydrogen permeation tests are enough to assess hydrogen free energies of trapping in the range of −35 kJ/mol to −70 kJ/mol. These conclusions are obtained by assumi...

  13. Apportion of Charpy energy in API 5L grade X70 pipeline steel

    Energy Technology Data Exchange (ETDEWEB)

    Hashemi, Sayyed H. [Department of Mechanical Engineering, The University of Birjand, PO Box 97175 /615, Birjand (Iran, Islamic Republic of)], E-mail: shhashemi@birjand.ac.ir

    2008-12-15

    Conventional Charpy based failure models for gas transportation pipelines recommend the minimum fracture energy for safe performance of these structures. In recent years however, full-scale burst experiments have shown that such models cannot fully guarantee the safety of higher grade pipeline steels. One possible reason for this discrepancy, which is further investigated in this research, is that Charpy energy inherently contains both fracture and non-fracture related energy. To separate this, energy partitioning analysis was used. First, the overall fracture energy of X70 steel is measured experimentally on an instrumented Charpy rig. Next, the measured energy is divided into fracture initiation and propagation parts using load-displacement data. It appeared from test results that a significant amount of energy was consumed in non-fracture related processes. From this, correction factors were suggested for possible use in current industry failure models. Interestingly, these corrections factors agreed well with those reported from full-thickness burst tests for tough pipeline steels.

  14. Google Ajax Search API

    CERN Document Server

    Fitzgerald, Michael

    2007-01-01

    Use the Google Ajax Search API to integrateweb search, image search, localsearch, and other types of search intoyour web site by embedding a simple, dynamicsearch box to display search resultsin your own web pages using a fewlines of JavaScript. For those who do not want to write code,the search wizards and solutions builtwith the Google Ajax Search API generatecode to accomplish common taskslike adding local search results to a GoogleMaps API mashup, adding videosearch thumbnails to your web site, oradding a news reel with the latest up todate stories to your blog. More advanced users can

  15. Corporate Consumer Contact API

    Data.gov (United States)

    General Services Administration — The data in the Corporate Consumer Contact API is based on the content you can find in the Corporate Consumer Contact listing in the Consumer Action Handbook (PDF)....

  16. Mars Rover Photos API

    Data.gov (United States)

    National Aeronautics and Space Administration — This API is designed to collect image data gathered by NASA's Curiosity, Opportunity, and Spirit rovers on Mars and make it more easily available to other...

  17. GIBS API for Developers

    Data.gov (United States)

    National Aeronautics and Space Administration — API using Global Imagery Browse Services (GIBS) designed to deliver global, full-resolution satellite imagery to users in a highly responsive manner, enabling...

  18. IBM System i APIs at Work

    CERN Document Server

    Vining, Bruce; Hawkins, Ron

    2007-01-01

    This new edition of the bestselling i5 application programming interface (API) book includes powerful new APIs and updated code to give programmers a working knowledge of the APIs available under i5/OS. Using real business-world examples, this book explains APIs that serve webpages, list APIs, APIs that work with user spaces, dynamic index APIs, APIs that retrieve system values, job information and job queue information APIs, APIs that work with data queues, APIs for error handling and message handling, and spool file APIs, among many others. Completely revised and including eight new chapters

  19. Trasplante cardíaco Heart transplant

    Directory of Open Access Journals (Sweden)

    M. Ubilla

    2006-08-01

    Full Text Available El trasplante cardíaco es considerado actualmente como el tratamiento de elección en la insuficiencia cardíaca terminal refractaria a tratamiento médico o quirúrgico. Debido a factores como la mayor esperanza de vida de la población y el manejo más eficaz de los síndromes coronarios agudos, cada vez hay un número mayor de personas que padecen fallo cardíaco. Se estima que la prevalencia de la enfermedad en países desarrollados está en torno al 1%; de éstos, un 10% está en una etapa avanzada y por tanto son potenciales receptores de un trasplante cardíaco. El problema está en que aún no es posible ofrecer esta modalidad terapéutica a todos los pacientes que la requieren. Por consiguiente, se hace necesario optimizar los resultados del trasplante cardíaco mediante la selección de pacientes, selección y manejo de los donantes, manejo perioperatorio y control de la enfermedad por rechazo del injerto. Desde el primer trasplante efectuado en diciembre de 1967, numerosos avances y cambios se han producido, lo que ha permitido aumentar la supervivencia y calidad de vida de quienes han recibido un nuevo corazón. A continuación se revisarán los aspectos más relevantes del trasplante cardíaco y los desafíos que enfrenta en la actualidad.A heart transplant is at present considered the treatment of choice in cases of terminal cardiac insufficiency refractory to medical or surgical treatment. Due to factors such as the greater life expectancy of the population and the more efficient management of acute coronary syndromes, there is an increasing number of people who suffer from heart failure. It is estimated that the prevalence of the disease in developed countries is around 1%; of this figure, some 10% are in an advanced stage and are thus potential receptors of a heart transplant. The problem is that it is still not possible to offer this therapeutic form to all of the patients that require it. Consequently, it is necessary to

  20. Contracting the Facebook API

    Directory of Open Access Journals (Sweden)

    Ben Rubinger

    2010-09-01

    Full Text Available In recent years, there has been an explosive growth in the popularity of online social networks such as Facebook. In a new twist, third party developers are now able to create their own web applications which plug into Facebook and work with Facebook's "social" data, enabling the entire Facebook user base of more than 400 million active users to use such applications. These client applications can contain subtle errors that can be hard to debug if they misuse the Facebook API. In this paper we present an experience report on applying Microsoft's new code contract system for the .NET framework to the Facebook API.We wrote contracts for several classes in the Facebook API wrapper which allows Microsoft .NET developers to implement Facebook applications. We evaluated the usefulness of these contracts during implementation of a new Facebook application. Our experience indicates that having code contracts provides a better and quicker software development experience.

  1. Mars Atmospheric Aggregation System API

    Data.gov (United States)

    National Aeronautics and Space Administration — The {MAAS} API is an open source REST API built to help make it easier and more efficient to build interactive applications that want to utilize the wealth of...

  2. Data.gov CKAN API

    Data.gov (United States)

    General Services Administration — The data.gov catalog is powered by CKAN, a powerful open source data platform that includes a robust API. Please be aware that data.gov and the data.gov CKAN API...

  3. ALOHA Cabled Observatory (ACO): Acoustic Doppler Current Profiler (ADCP): Temperature

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The University of Hawaii's ALOHA ("A Long-term Oligotrophic Habitat Assessment") Cabled Observatory (ACO) is located 100 km north of the island of Oahu, Hawaii (22...

  4. ALOHA Cabled Observatory (ACO): Acoustic Doppler Current Profiler (ADCP): Velocity

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The University of Hawaii's ALOHA ("A Long-term Oligotrophic Habitat Assessment") Cabled Observatory (ACO) is located 100 km north of the island of Oahu, Hawaii (22...

  5. A hybrid ACO algorithm for the full truckload transportation problem

    OpenAIRE

    Doerner, Karl; Hartl, Richard F.; Reimann, Marc

    2001-01-01

    In this paper we propose a hybrid ACO approach to solve a full truckload transportation problem. Hybridization is achieved through the use of a problem specific heuristic. This heuristic is utilized both, to initialize the pheromone information and to construct solutions in the ACO pro-cedure. The main idea is to use information about the required fleetsize, by initializing the system with a number of vehicles rather than opening vehicles one at a time as needed. Our results show the advantag...

  6. Web API Fragility: How Robust is Your Web API Client

    NARCIS (Netherlands)

    Espinha, T.; Zaidman, A.; Gross, H.G.

    2014-01-01

    Web APIs provide a systematic and extensible approach for application-to-application interaction. A large number of mobile applications makes use of web APIs to integrate services into apps. Each Web API’s evolution pace is determined by their respective developer and mobile application developers a

  7. Learning Bing maps API

    CERN Document Server

    Sinani, Artan

    2013-01-01

    This is a practical, hands-on guide with illustrative examples, which will help you explore the vast universe of Bing maps.If you are a developer who wants to learn how to exploit the numerous features of Bing Maps then this book is ideal for you. It can also be useful for more experienced developers who wish to explore other areas of the APIs. It is assumed that you have some knowledge of JavaScript, HTML, and CSS. For some chapters a working knowledge of .Net and Visual Studio is also needed.

  8. AUTOMATED API TESTING APPROACH

    Directory of Open Access Journals (Sweden)

    SUNIL L. BANGARE

    2012-02-01

    Full Text Available Software testing is an investigation conducted to provide stakeholders with information about the quality of the product or service under test. With the help of software testing we can verify or validate the software product. Normally testing will be done after development of software but we can perform the software testing at the time of development process also. This paper will give you a brief introduction about Automated API Testing Tool. This tool of testing will reduce lots of headache after the whole development of software. It saves time as well as money. Such type of testing is helpful in the Industries & Colleges also.

  9. Contracting the Facebook API

    OpenAIRE

    2010-01-01

    In recent years, there has been an explosive growth in the popularity of online social networks such as Facebook. In a new twist, third party developers are now able to create their own web applications which plug into Facebook and work with Facebook's "social" data, enabling the entire Facebook user base of more than 400 million active users to use such applications. These client applications can contain subtle errors that can be hard to debug if they misuse the Facebook API. In this paper w...

  10. Paro Cardíaco en el Embarazo

    OpenAIRE

    Manuel Eduardo Sáenz Madrigal; Carlos Adrián Vindas Morera

    2013-01-01

    El paro cardíaco en el embarazo presenta un escenario único en el que están incluidos dos pacientes: la madre y el feto. El manejo de este escenario requiere de un equipo multidisciplinario incluyendo especialistas en anestesia, obstetricia, neonatología, cardiología y en ocasiones cirugía cardíaca. Los protocolos de soporte vital básico y soporte cardíaco avanzado deben ser implementados, sin embargo, dados los cambios anatómicos y fisiológicos que ocurren en el embarazo, algunas modificacio...

  11. Twitter manager GO API intended for GNU Social API

    OpenAIRE

    Janiga, Jaroslav

    2015-01-01

    The bachelor’s thesis had two goals. The first one was to create API for GO programming language that communicates with GNU Social API. The second one was to create an application that uses the mentioned API. The main purpose of creating the application was to reduce the time spent with the login and logout of the accounts by the members in the white team. The application is intended for training purposes in JYVSECTEC project at JAMK University of Applied Sciences. JYVSECTEC sta...

  12. A Review on Genomics APIs

    Directory of Open Access Journals (Sweden)

    Rajeswari Swaminathan

    2016-01-01

    Full Text Available The constant improvement and falling prices of whole human genome Next Generation Sequencing (NGS has resulted in rapid adoption of genomic information at both clinics and research institutions. Considered together, the complexity of genomics data, due to its large volume and diversity along with the need for genomic data sharing, has resulted in the creation of Application Programming Interface (API for secure, modular, interoperable access to genomic data from different applications, platforms, and even organizations. The Genomics APIs are a set of special protocols that assist software developers in dealing with multiple genomic data sources for building seamless, interoperable applications leading to the advancement of both genomic and clinical research. These APIs help define a standard for retrieval of genomic data from multiple sources as well as to better package genomic information for integration with Electronic Health Records. This review covers three currently available Genomics APIs: a Google Genomics, b SMART Genomics, and c 23andMe. The functionalities, reference implementations (if available and authentication protocols of each API are reviewed. A comparative analysis of the different features across the three APIs is provided in the Discussion section. Though Genomics APIs are still under active development and have yet to reach widespread adoption, they hold the promise to make building of complicated genomics applications easier with downstream constructive effects on healthcare.

  13. Molecular Evolution and Expression Divergence of Aconitase (ACO Gene Family in Land Plants

    Directory of Open Access Journals (Sweden)

    Yi-ming Wang

    2016-12-01

    Full Text Available Aconitase (ACO is a key enzyme that catalyzes the isomerization of citrate to isocitrate in the tricarboxylic acid (TCA and glyoxylate cycles. The function of ACOs has been well studied in model plants, such as Arabidopsis. In contrast, the evolutionary patterns of the ACO family in land plants are poorly understood. In this study, we systematically examined the molecular evolution and expression divergence of the ACO gene family in 12 land plant species. Thirty-six ACO genes were identified from the 12 land plant species representing the four major land plant lineages: bryophytes, lycophytes, gymnosperms, and angiosperms. All of these ACOs belong to the cytosolic isoform. Three gene duplication events contributed to the expansion of the ACO family in angiosperms. The ancestor of angiosperms may have contained only one ACO gene. One gene duplication event split angiosperm ACOs into two distinct clades. Two clades showed a divergence in selective pressure and gene expression patterns. The cis-acting elements that function in light responsiveness were most abundant in the promoter region of the ACO genes, indicating that plant ACO genes might participate in light regulatory pathways. Our findings provide comprehensive insights into the ACO gene family in land plants.

  14. Intro to the Zotero API

    Directory of Open Access Journals (Sweden)

    Amanda Morton

    2013-04-01

    Full Text Available In this lesson, you’ll learn how to use python with the Zotero API to interact with your Zotero library. The Zotero API is a powerful interface that would allow you to build a complete Zotero client from scratch if you so desired. But like most APIs, it works in small, discrete steps, so we have to build our way up to the complicated requests we might want to use to access our Zotero libraries. But this incremental building gives us plenty of time to learn as we go along.

  15. Paro Cardíaco en el Embarazo

    Directory of Open Access Journals (Sweden)

    Manuel Eduardo Sáenz Madrigal

    2013-12-01

    Full Text Available El paro cardíaco en el embarazo presenta un escenario único en el que están incluidos dos pacientes: la madre y el feto. El manejo de este escenario requiere de un equipo multidisciplinario incluyendo especialistas en anestesia, obstetricia, neonatología, cardiología y en ocasiones cirugía cardíaca. Los protocolos de soporte vital básico y soporte cardíaco avanzado deben ser implementados, sin embargo, dados los cambios anatómicos y fisiológicos que ocurren en el embarazo, algunas modificaciones en los algoritmos son fundamentales. La evidencia existente acerca del manejo del paro cardíaco en el embarazo es relativamente insuficiente, sin estudios randomizados, por lo tanto las recomendaciones son basadas en pequeños estudios de cohorte y reportes de casos, además de la opinión de los expertos. En esta revisión hablaremos del paro cardíaco en el embarazo, sus implicaciones y el manejo adecuado por parte del equipo multidisciplinario, además del tiempo en el que se debe realizar la cesárea en caso de no retorno de circulación espontánea.

  16. First Order Deceptive Problem of ACO and Its Performance Analysis

    Directory of Open Access Journals (Sweden)

    Ling Chen

    2009-12-01

    Full Text Available Ant colony optimization(ACO, which is one of the intelligential optimization algorithm, has been widely used to solve combinational optimization problems. Deceptive problems have been considered difficult for ant colony optimization. It was believed that ACO will fail to converge to global optima of deceptive problems. This paper proves that the first order deceptive problem of ant colony algorithm satisfies value convergence under certain initial pheromone distribution, but does not satisfy solution convergence. We also present a first attempt towards the value-convergence time complexity analysis of ACO on the first-order deceptive systems taking the n-bit trap problem as the test instance. We prove that time complexity of MMAS, which is an ACO with limitations of the pheromone on each edge, on n-bit trap problem is O(n2m.log n, here n is the size of the problem and m is the number of artificial ants. Our experimental results confirm the correctness of our analysis.

  17. Improving Network Performance using ACO Based Redundant Link Avoidance Algorithm

    Directory of Open Access Journals (Sweden)

    B. Chandra Mohan

    2010-05-01

    Full Text Available In the wide spread internet, response time and pocket loss are inappropriate due to network traffic, as a result the network efficiency becomes worst and the system provides poor Quality of Service (QoS. An optimal routing protocol, especially multipath may avoid such traffic in the network. But existing routing protocols, both single path and multi path, concentrates only on finding the routes based on any one or some set of metrics, that not always suitable for dynamic, cloud natured network environment. Ant Colony Optimization (ACO based multipath routing protocol was suggested as an alternate to this problem by many researchers. The multipath ACO also provides same set of link(s for the source to destination, so that traffic merging again becomes a critical problem. This paper proposes an optimal solution to avoid the problem of traffic merging in the network by removing redundant link in the route. The Proposed algorithm, called 'Redundant Link Avoidance (RLA algorithm', is an ACO based multi path routing methodology, avoiding copious link in the suggested routes of ACO multipath protocol.

  18. Cold bending of 34'' OD API 5L X80 pipes; Curvamento a frio de tubos API 5L X80 de 34'' de diametro

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Ronaldo; Moreira, Fabio A.; Chad, Luis; Fritz, Marcelo C. [TenarisConfab, Pindamonhangaba, SP (Brazil); Batista, Gilmar Z.; Hippert, Eduardo [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)

    2008-07-01

    A key factor that demands special attention in the pipeline construction is the cold bending process, since 30 to 40% of the pipes use this process in the field. This study aimed to evaluate the X80 cold bending operational parameters, in order make viable the use of this process in the installation of future onshore pipelines. Three 34''OD x 0,750'' pipes were bended. The bending was conducted using a hydraulic equipment with application of equally spaced punches, recording the correspondent angles related to the elastic and plastic deformations in order to assess the spring-back effect and performing dimensional inspection. Samples from pipe and the weld were subjected to mechanical and metallographic tests. It was possible to obtain a 19 deg curve and 27D radius without presenting any evidence of wrinkles, out of roundness or any type of mechanical damage. After analysis, all criteria established from project specifications were complied, the mechanical tests performed allowed comparing pipe mechanical properties of straight and bended regions. The results of this study provide technical know how for future X80 onshore pipelines applications. (author)

  19. ACO in e-Learning: Towards an adaptive learning path

    Directory of Open Access Journals (Sweden)

    Pushpa. M

    2012-03-01

    Full Text Available Today we are in an era where drastic advancements in networking and information technology are in action. The learning process has also taken these advancements, as a result of which e-learning came to thescene. Personalization in e-learning will improve the performance of the system. Recent researches are concentrating on providing adaptability to the learning management systems, depending upon the varying user needs and contexts. Adaptability can be provided at different levels .Providing an adaptive learning path according to the context of the learners’ is an important issue. An optimal adaptive learning path will help the learners in reducing the cognitive overload and disorientation, and thereby improving the efficiency of the Learning Management System (LMS. Ant Colony Optimization (ACO is a widely accepted technique since it provides an adaptive learning path to the learners. Meta-heuristic which is used in intelligent tutoring systems provides the learning path in an adaptive way. The most interesting feature of ACO is its adaptation and robustness in an environment where the learning materials and learners are changing frequently. In this paper we can have a look through the existing ACO approaches towards providing an adaptive learning path and an introduction towards an enhanced attribute ant for making the e-learning system more adaptive.

  20. Automated Ply Inspection (API) for AFP Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Automated Ply Inspection (API) system autonomously inspects layups created by high speed automated fiber placement (AFP) machines. API comprises a high accuracy...

  1. USAJOBS Job Opportunity Announcements (JOA) SOAP API

    Data.gov (United States)

    Office of Personnel Management — The purpose of the SOAP based API is to provide the full Federal Job Opportunity Announcement (JOA) content to the consumer. It is anticipated that this API will be...

  2. USAJOBS Job Opportunity Announcements (JOA) REST API

    Data.gov (United States)

    Office of Personnel Management — This REST-based API is designed to support lightweight Federal Job Opportunity Announcement (JOA) content consumption by consumers. It is anticipated that this API...

  3. Dolor mandibular de origen cardíaco

    OpenAIRE

    2003-01-01

    En las complejas estructuras anatómicas orofaciales, asientan muchos procesos dolorosos. Las algias orofaciales más comunes tienen su origen a nivel dental, periodontal o en estructuras musculoesqueleticas. Sin embargo, el paciente puede manifestar dolor en esta región, bien en dientes o estructuras musculoesqueleticas, y la fuente originaria localizarse a distancia. A este tipo de dolor se le llama dolor heterotópico. Una posible fuente de dolor heterotópico es el dolor de origen cardíaco. E...

  4. MODELO ACO PARA LA RECOLECCIÓN DE RESIDUOS POR CONTENEDORES ACO MODEL APPLIED TO THE WASTE COLLECTION BY CONTAINERS

    Directory of Open Access Journals (Sweden)

    Eduardo Salazar Hornig

    2009-08-01

    Full Text Available ACO es una metaheurística inspirada en el comportamiento de las colonias de hormigas para solucionar problemas de optimización combinatoria, por medio de la utilización de agentes computacionales simples que trabajan de manera cooperativa y se comunican mediante rastros de feromona artificiales. En este trabajo se presenta un modelo para resolver el Problema de Recolección de Residuos Domiciliarios por Contenedores, el que aplica un concepto de secuencias parciales de recolección que deben ser unidas para minimizar la distancia total de recolección. El problema de unir las secuencias parciales se representa como un TSP, el que es resuelto mediante un algoritmo ACO. En base a recomendaciones de la literatura, se calibran experimentalmente los parámetros del algoritmo y se recomiendan rangos de valores que representan buenos rendimientos promedio. El modelo se aplica a un sector de recolección de la comuna de San Pedro de la Paz, Chile, obteniéndose rutas de recolección que reducen la distancia total recorrida respecto de la actual ruta utilizada y de la solución obtenida con otro modelo desarrollado previamente.ACO is a metaheuristic inspired in the behavior of natural ant colonies to solve combinatorial optimization problems, based on simple agents that work cooperatively communicating by artificial pheromone trails. In this paper a model to solve the municipal waste collection problem by containers is presented, which applies a concept of partial collection sequences that must be joined to minimize the total collection distance. The problem to join the partial collection sequences is represented as a TSP, which is solved by an ACO algorithm. Based on the literature, algorithm parameters are experimentally calibrated and range of variations that represents good average solutions are recommended. The model is applied to a waste collection sector of the San Pedro de la Paz commune in Chile, obtaining recollection routes with less total

  5. ENHANCED HYBRID PSO – ACO ALGORITHM FOR GRID SCHEDULING

    Directory of Open Access Journals (Sweden)

    P. Mathiyalagan

    2010-07-01

    Full Text Available Grid computing is a high performance computing environment to solve larger scale computational demands. Grid computing contains resource management, task scheduling, security problems, information management and so on. Task scheduling is a fundamental issue in achieving high performance in grid computing systems. A computational GRID is typically heterogeneous in the sense that it combines clusters of varying sizes, and different clusters typically contains processing elements with different level of performance. In this, heuristic approaches based on particle swarm optimization and ant colony optimization algorithms are adopted for solving task scheduling problems in grid environment. Particle Swarm Optimization (PSO is one of the latest evolutionary optimization techniques by nature. It has the better ability of global searching and has been successfully applied to many areas such as, neural network training etc. Due to the linear decreasing of inertia weight in PSO the convergence rate becomes faster, which leads to the minimal makespan time when used for scheduling. To make the convergence rate faster, the PSO algorithm is improved by modifying the inertia parameter, such that it produces better performance and gives an optimized result. The ACO algorithm is improved by modifying the pheromone updating rule. ACO algorithm is hybridized with PSO algorithm for efficient result and better convergence in PSO algorithm.

  6. Report on the 10th International Conference of the Asian Clinical Oncology Society (ACOS 2012).

    Science.gov (United States)

    Kim, Yeul Hong; Yang, Han-Kwang; Kim, Tae Won; Lee, Jung Shin; Seong, Jinsil; Lee, Woo Yong; Ahn, Yong Chan; Lim, Ho Yeong; Won, Jong-Ho; Park, Kyong Hwa; Cho, Kyung Sam

    2013-04-01

    The 10th International Conference of the Asian Clinical Oncology Society (ACOS 2012) in conjunction with the 38th Annual Meeting of the Korean Cancer Association, was held on June 13 to 15 (3 days) 2012 at COEX Convention and Exhibition Center in Seoul, Korea. ACOS has a 20-year history starting from the first conference in Osaka, Japan, which was chaired by Prof. Tetsuo Taguchi and the ACOS conferences have since been conducted in Asian countries every 2 years. Under the theme of "Work Together to Make a Difference for Cancer Therapy in Asia", the 10th ACOS was prepared to discuss various subjects through a high-quality academic program, exhibition, and social events. The ACOS 2012 Committee was composed of the ACOS Organizing Committee, Honorary Advisors, Local Advisors, and ACOS 2012 Organizing Committee. The comprehensive academic program had a total of 92 sessions (3 Plenary Lectures, 1 Award Lectures, 1 Memorial Lectures, 9 Special Lectures, 15 Symposia, 1 Debate & Summary Sessions, 1 Case Conferences, 19 Educational Lectures, 1 Research & Development Session, 18 Satellite Symposia, 9 Meet the Professors, 14 Oral Presentations) and a total 292 presentations were delivered throughout the entire program. Amongst Free Papers, 462 research papers (110 oral presentations and 352 poster presentations) were selected to be presented. This conference was the largest of all ACOS conferences in its scale with around 1,500 participants from 30 countries. Furthermore, despite strict new financial policies and requirements governing fundraising alongside global economic stagnation, a total of 14 companies participated as sponsors and an additional 35 companies purchased 76 exhibition booths. Lastly, the conference social events provided attendees with a variety of opportunities to experience and enjoy Korea's rich culture and traditions during the Opening Ceremony, Welcome Reception, Invitee Dinner, Banquet, and Closing Ceremony. Overall, ACOS 2012 reinforced and promoted

  7. Automated information extraction from web APIs documentation

    OpenAIRE

    Ly, Papa Alioune; Pedrinaci, Carlos; Domingue, John

    2012-01-01

    A fundamental characteristic of Web APIs is the fact that, de facto, providers hardly follow any standard practices while implementing, publishing, and documenting their APIs. As a consequence, the discovery and use of these services by third parties is significantly hampered. In order to achieve further automation while exploiting Web APIs we present an approach for automatically extracting relevant technical information from the Web pages documenting them. In particular we have devised two ...

  8. Accessing Inaccessible Android APIs: An Empirical Study

    OpenAIRE

    Li, LI; Bissyande, Tegawendé François D Assise; Le Traon, Yves; Klein, Jacques

    2016-01-01

    As Android becomes a de-facto choice of development platform for mobile apps, developers extensively leverage its accompanying Software Development Kit to quickly build their apps. This SDK comes with a set of APIs which developers may find limited in comparison to what system apps can do or what framework developers are preparing to harness capabilities of new generation devices. Thus, developers may attempt to explore in advance the normally “inaccessible” APIs for building unique API-based...

  9. BusinessUSA Resource Access API

    Data.gov (United States)

    General Services Administration — BusinessUSA connects businesses to government services and information. The API provides access to resource abstracts including programs, services, data, events, and...

  10. AKTIVITAS ANTIOKSIDAN DAN EFEK HEPATOPROTEKTIF DAUN BAKAU API-API PUTIH

    Directory of Open Access Journals (Sweden)

    safrina dyah hardiningtyas

    2014-07-01

    Full Text Available Penelitian ini bertujuan untuk mendapatkan ekstrak kasar daun api-api putih yang memiliki aktivitas antioksidan terbaik, menentukan kandungan fitokimia dan efek Hepatoprotektif ekstrak kasar yang dipilih secara in vivo. Daun api-api putih diekstrak menggunakan tiga jenis pelarut, yakni metanol, etil asetat dan n-heksana. Dalam uji in vivo dilakukan evaluasi terhadap kadar malondialdehid (MDA, enzim aspartat transaminase (AST, enzim alanin transaminase (ALT, dan histopatologi. Hasil penelitian menunjukkan bahwa ekstraksi daun api-api putih dengan pelarut etil asetat menghasilkan aktivitas antioksidan terbaik dengan kandungan fitokimia terdiri dari flavonoid dan steroid/triterpenoid. Pemberian ekstrak etil asetat daun api-api putih pada tikus yang telah diinduksi CCl4 dapat menormalkan berbagai parameter biokimia stres oksidatif (kadar MDA, AST, dan ALT dibandingkan dengan standar Silymarin. Ekstrak daun api-api putih berpotensi untuk melindungi hati tikus dari kerusakan oksidatif yang diinduksi CCl4. Efek Hepatoprotektif ekstrak daun api-api putih berkorelasi dengan aktivitas antioksidannya.Kata kunci: antioksidan, Avicennia marina, ekstrak, hepatoprotektor

  11. PAPR reduction scheme for ACO-OFDM based visible light communication systems

    Science.gov (United States)

    zhang, Tian; Ghassemlooy, Zabih; Ma, Chunyang; Guo, Shuxu

    2017-01-01

    In this paper, we proposed a novel peak to average power ratio (PAPR) reduction scheme for the asymmetrically clipped optical orthogonal frequency division multiplexing (ACO-OFDM) visible light communications system. We implement the Toeplitz matrix based Gaussian blur method to reduce the high PAPR of ACO-OFDM at the transmitter and use the orthogonal matching pursuit algorithm to recover the original ACO-OFDM frame at the receiver. Simulation results show that for the 256-subcarrier ACO-OFDM system a 6 dB improvement in PAPR is achieved compared with the original ACO-OFDM in term of the complementary cumulative distribution function, while maintaining a competitive bit-error rate performance compared with the ideal ACO-OFDM lower bound. We also demonstrated the optimal parameter C of 2 for the recovery algorithm based on the tradeoff between the data rate and recovery accuracy. The recovery results show that using the proposed scheme the ACO-OFDM can faithfully be reconstructed judging by the very low value for the reconstruct error of 0.06.

  12. Layered ACO-OFDM for intensity-modulated direct-detection optical wireless transmission.

    Science.gov (United States)

    Wang, Qi; Qian, Chen; Guo, Xuhan; Wang, Zhaocheng; Cunningham, David G; White, Ian H

    2015-05-04

    Layered asymmetrically clipped optical orthogonal frequency division multiplexing (ACO-OFDM) with high spectral efficiency is proposed in this paper for optical wireless transmission employing intensity modulation with direct detection. In contrast to the conventional ACO-OFDM, which only utilizes odd subcarriers for modulation, leading to an obvious spectral efficiency loss, in layered ACO-OFDM, the subcarriers are divided into different layers and modulated by different kinds of ACO-OFDM, which are combined for simultaneous transmission. In this way, more subcarriers are used for data transmission and the spectral efficiency is improved. An iterative receiver is also proposed for layered ACO-OFDM, where the negative clipping distortion of each layer is subtracted once it is detected so that the signals from different layers can be recovered. Theoretical analysis shows that the proposed scheme can improve the spectral efficiency by up to 2 times compared with conventional ACO-OFDM approaches with the same modulation order. Meanwhile, simulation results confirm a considerable signal-to-noise ratio gain over ACO-OFDM at the same spectral efficiency.

  13. Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera

    Science.gov (United States)

    Gong, Hong-Ri; Chen, Xiu-Xian; Chen, Yan Ping; Hu, Fu-Liang; Zhang, Jiang-Lin; Lin, Zhe-Guang; Yu, Ji-Wei

    2016-01-01

    Sacbrood virus (SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera. In previous studies, SBV isolates infecting A. cerana (AcSBV) and SBV isolates infecting A. mellifera (AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined the presence of SBV infection in 318 A. mellifera colonies and 64 A. cerana colonies, and we identified the genotypes of SBV isolates. We also performed artificial infection experiments under both laboratory and field conditions. The results showed that 38 A. mellifera colonies and 37 A. cerana colonies were positive for SBV infection. Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) gene sequences indicated that A. cerana isolates and most A. mellifera isolates formed two distinct clades but two strains isolated from A. mellifera were clustered with the A. cerana isolates. In the artificial-infection experiments, AcSBV negative-strand RNA could be detected in both adult bees and larvae of A. mellifera, although there were no obvious signs of the disease, demonstrating the replication of AcSBV in A. mellifera. Our results suggest that AcSBV is able to infect A. mellifera colonies with low prevalence (0.63% in this study) and pathogenicity. This work will help explain the different susceptibilities of A. cerana and A. mellifera to sacbrood disease and is potentially useful for guiding beekeeping practices. PMID:26801569

  14. Validation of the Parlay API through prototyping

    NARCIS (Netherlands)

    Hellenthal, J.W.; Planken, F.J.M.; Wegdam, M.

    2001-01-01

    The desire within the telecommunications world for new and faster business growth has been a major drive towards the development of open network API. Over the past 7 years several (semi) standardization groups have announced work on network API, including TINA-C, JAIN, IEEE P1520, INforum, 3GPP, JAI

  15. DESIGNING ACO-BASED TRAVEL ITINERARY PLANNING SYSTEM WITH SMART TERMINAL%基于 ACO 的智能终端旅游行程规划系统设计

    Institute of Scientific and Technical Information of China (English)

    睢先先; 宋夫华

    2016-01-01

    Travel itinerary planning is a very complicated issue for self-service travellers.In light of this problem,we studied the itinerary planning model,itinerary planning algorithm and implementation schemes,and constructed a service platform for urban travel itinerary plan-ning.In combination with a variety of technologies such as ant colony optimisation (ACO),Baidu map API,Android development,WebSer-vice and JSON,etc.,we improved the AOC and realised the Android smart terminal-based urban self-service travel itinerary planning system. Experimental results showed that the implemented itinerary planning system with smart terminal has the advantages of high planning efficien-cy,reasonable planning results,intuitive information display and easy in use for travel itinerary planning within city region.It has certain popularisation value.%旅游行程规划对自助游客来说是一件非常繁琐的事情。针对该问题,对行程规划模型、行程规划算法和实现方案进行研究,构建城市内的旅游行程规划服务平台。结合蚁群优化 ACO(Ant Colony Optimization)算法、百度地图 API、Android 开发、WebSer-vice 和 JSON 等技术,改进 ACO 算法,实现了基于 Android 智能终端的城市内自助游行程规划系统。实验结果表明,实现的智能终端行程规划系统在城市区域内的旅游行程规划方面具有规划效率高、规划结果合理、信息展示直观、使用方便等优点,具有一定的推广价值。

  16. Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera.

    Science.gov (United States)

    Gong, Hong-Ri; Chen, Xiu-Xian; Chen, Yan Ping; Hu, Fu-Liang; Zhang, Jiang-Lin; Lin, Zhe-Guang; Yu, Ji-Wei; Zheng, Huo-Qing

    2016-04-01

    Sacbrood virus(SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera In previous studies, SBV isolates infecting A. cerana(AcSBV) and SBV isolates infecting A. mellifera(AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined the presence of SBV infection in 318A. mellifera colonies and 64A. cerana colonies, and we identified the genotypes of SBV isolates. We also performed artificial infection experiments under both laboratory and field conditions. The results showed that 38A. mellifera colonies and 37A. cerana colonies were positive for SBV infection. Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) gene sequences indicated that A. cerana isolates and most A. mellifera isolates formed two distinct clades but two strains isolated fromA. mellifera were clustered with theA. cerana isolates. In the artificial-infection experiments, AcSBV negative-strand RNA could be detected in both adult bees and larvae ofA. mellifera, although there were no obvious signs of the disease, demonstrating the replication of AcSBV inA. mellifera Our results suggest that AcSBV is able to infectA. melliferacolonies with low prevalence (0.63% in this study) and pathogenicity. This work will help explain the different susceptibilities ofA. cerana and A. melliferato sacbrood disease and is potentially useful for guiding beekeeping practices.

  17. Methodology to Optimize Manufacturing Time for a CNC Using a High Performance Implementation of ACO

    Directory of Open Access Journals (Sweden)

    Oscar Montiel-Ross

    2012-10-01

    Full Text Available In this paper, an efficient methodology to generate optimal and/or quasi‐optimal sequences of G commands to minimize the manufacturing time is presented. Our solution starts from original G codes provided by application CAD/CAM software. Here, first we tackled the problem of reducing the time of the travel path for drilling of an industrial robotic manufacturing machine. The methodology can be easily implemented for free distribution or commercial CAD/CAM software without achieving any modification to it. Several experiments that demonstrate how this proposal can help to outperform solutions provided by application software are presented, consistent improvements around 62% were obtained. Moreover, for optimizing the time along the travel path, we present a high performance implementation of Ant Colonies (ACO known as Parallel ACO (P‐ACO that allows achieving the optimization task efficiently by speeding up the original ACO. A Graphical User Interface that integrates the whole process is shown.

  18. Presenting Provenance Based on User Roles - Experiences from the ACOS System

    Science.gov (United States)

    West, P.; Michaelis, J.; Fox, P. A.; Zednik, S.; McGuinness, D. L.

    2010-12-01

    One goal of provenance is to provide users an understanding of the steps a system took to generate data products. Here, the level of detail captured by provenance becomes an important consideration. As detail is added, more questions can be hypothetically addressed. However, presenting significant provenance detail may also overwhelm end users, for one of two reasons: (i) the detail presented is irrelevant to the objectives, or (ii) the detail requires background knowledge a user lacks. Both of these challenges are present for data generated by the Mauna Loa Solar Observatory’s (MLSO) Advanced Coronal Observing System (ACOS). In ACOS, photometer-based readings are taken of solar activity and subsequently processed into data products consumable by end users. To fully understand these sequences of steps, background knowledge corresponding to various areas (e.g., astronomy, digital imaging, and ACOS specific techniques) is required by end users. This makes reviewing provenance difficult for users outside the ACOS development team, where varying degrees of background may be expected (ranging from outside domain experts in Solar Physics to citizen scientists). Likewise, even when steps taken by ACOS are understandable, they may provide undesired detail to an end user if presented. The work with ACOS involved the development of a Semantic Web based framework to selectively present provenance detail for data products in ACOS. Here, provenance is captured according to two sets of ontologies, the Proof Markup Language, which is an ontology based domain-independent provenance model, and a step ontology, designed to capture hierarchies of provenance steps. Used in combination, these ontology sets enable the creation of multiple levels of provenance, ranging from coarse to fine grained detail. In this setting, users may choose to expand/collapse provenance steps to view desired details. However, the specific provenance details a user initially sees is defined through

  19. How do developers use APIs? A case study in concurrency

    NARCIS (Netherlands)

    Blom, Stefan; Kiniry, Joseph; Huisman, Marieke

    2013-01-01

    With the omnipresent usage of APIs in software development, it has become important to analyse how the routines and functionalities of APIs are actually used. This information is in particular useful for API developers, to make decisions about future updates of the API. However, also for developers

  20. Web API Growing Pains: Loosely Coupled yet Strongly Tied

    NARCIS (Netherlands)

    Espinha, T.; Zaidman, A.; Gross, H.G.

    2014-01-01

    Web APIs provide a systematic and extensible approach for application-toapplication interaction. Developers using web APIs are forced to accompany the API providers in their software evolution tasks. In order to understand the distress caused by this imposition on web API client developers we perfor

  1. Analysis and Applications API eBay

    OpenAIRE

    ŠIK, Martin

    2013-01-01

    The subject of this bachelor thesis "Analysis and Applications API eBay" is to create application based on the use of Application Programming Interface (API), released by eBay. The theoretical part is focused on explaining the fundamental issue of Internet auctions, e-commmerce, comparsion of auction portals and term "trust" as a key attribute of e-commerce. The practical part is based on analyse of principles and instruments of eBay API and create an application based on this interface. The ...

  2. DySectAPI: Scalable Prescriptive Debugging

    DEFF Research Database (Denmark)

    Jensen, Nicklas Bo; Karlsson, Sven; Quarfot Nielsen, Niklas;

    We present the DySectAPI, a tool that allow users to construct probe trees for automatic, event-driven debugging at scale. The traditional, interactive debugging model, whereby users manually step through and inspect their application, does not scale well even for current supercomputers. While...... lightweight debugging models scale well, they can currently only debug a subset of bug classes. DySectAPI fills the gap between these two approaches with a novel user-guided approach. Using both experimental results and analytical modeling we show how DySectAPI scales and can run with a low overhead...

  3. Dolor mandibular de origen cardíaco

    Directory of Open Access Journals (Sweden)

    MR Sáez Yuguero

    2003-10-01

    Full Text Available En las complejas estructuras anatómicas orofaciales, asientan muchos procesos dolorosos. Las algias orofaciales más comunes tienen su origen a nivel dental, periodontal o en estructuras musculoesqueleticas. Sin embargo, el paciente puede manifestar dolor en esta región, bien en dientes o estructuras musculoesqueleticas, y la fuente originaria localizarse a distancia. A este tipo de dolor se le llama dolor heterotópico. Una posible fuente de dolor heterotópico es el dolor de origen cardíaco. Este articulo presenta un caso clínico en el que el dolor mandibular bilateral fue el síntoma inicial de una cardiopatía isquémica que fue erróneamente etiquetada de artrosis de la articulación temporomandibular.In the complex orofacial anatomic structures, many painful processes are felt. The most common orofacial pains have their origin at dental or periodontal level, or in muscle -skeletical structures. However, the patient can have pain in this area- either in teeth or muscle-eskeletical structures- and the originating source can be located at a distance. This type of pain is called heterotopic pain. A possible source of heterotopic pain is the pain of cardiac origin. This paper presents a clinical case in which the bilateral mandibular pain is the initial symptom of an ischemic cardiopathy that was erroneously labeled as temporomandibular arthrosis.

  4. Real World Uses For Nagios APIs

    Science.gov (United States)

    Singh, Janice

    2014-01-01

    This presentation describes the Nagios 4 APIs and how the NASA Advanced Supercomputing at Ames Research Center is employing them to upgrade its graphical status display (the HUD) and explain why it's worth trying to use them yourselves.

  5. BeXRB Monitor Data API

    Data.gov (United States)

    National Aeronautics and Space Administration — A fully queryable REST API with JSON, XML, and CSV output as well as inline, runable examples. This is a monitor using data from Swift/BAT, MAXI and Fermi/GBM...

  6. ChemSpell Web Service API

    Data.gov (United States)

    U.S. Department of Health & Human Services — The ChemSpell Web Service API provides chemical name spell checking and chemical name synonym look-up. ChemSpell contains more than 1.3 million chemical names...

  7. System for Award Management (SAM) API

    Data.gov (United States)

    General Services Administration — The SAM API is a RESTful method of retrieving public information about the businesses, organizations, or individuals (referred to as entities) within the SAM entity...

  8. RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit.

    Science.gov (United States)

    Sekeli, Rogayah; Abdullah, Janna Ong; Namasivayam, Parameswari; Muda, Pauziah; Abu Bakar, Umi Kalsom; Yeong, Wee Chien; Pillai, Vilasini

    2014-06-19

    The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6). Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants.

  9. RNA Interference of 1-Aminocyclopropane-1-carboxylic Acid Oxidase (ACO1 and ACO2 Genes Expression Prolongs the Shelf Life of Eksotika (Carica papaya L. Papaya Fruit

    Directory of Open Access Journals (Sweden)

    Rogayah Sekeli

    2014-06-01

    Full Text Available The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6. Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants.

  10. 基于ACO-PAM综合算法的电力负荷聚类分析%Power load clustering analysis based on ACO-PAM synthesis algorithm

    Institute of Scientific and Technical Information of China (English)

    刘建华; 王进; 杨洪春; 孟颖

    2011-01-01

    Load characteristics classification and synthesis play an important role in practical load modeling. In order to establish a proper substation load model, an ACO-PAM based synthetic algorithm applying clustering method for load characteristics analysis is proposed in this paper. The algorithm makes clustering analysis for history optimal position of ACO, and it replaces the reference point for the new clustering center. The clustering data can be clustered adaptively to the classification. The ACO algorithm has strong global search ability and is easy to combine with other methods. It improves the shortcomings of PAM, such as easily sinking into local optimum and poor clustering effect. Finally, cases analysis results show that the synthesis algorithm has high feasibility and effectivity.%负荷特性分类与综合是实现负荷模型实用化的关键.为建立合适的变电站负荷模型,将聚类方法引入负荷特性分析,提出一种基于ACO-PAM的综合聚类算法.该综合算法是PAM算法对蚁群的历史最优位置进行聚类分析,将此位置代替PAM的参考点,作为新的聚类中心,数据将自适应地加入到适合它的聚类中.ACO算法具有全局搜索能力强、易于与其他方法结合的优点,改进了PAM算法易陷入局部最优、实际数据聚类效果差等不足.实例分析验证了ACO-PAM综合算法应用的可行性和有效性.

  11. A Few Thoughts about ACO Antitrust Issues from a Local Enforcement Perspective.

    Science.gov (United States)

    Foote, Kathleen E; Varanini, Emilio E

    2015-08-01

    Accountable care organizations (ACOs), joint ventures of commercial insurers and various groups of medical providers such as physicians, specialists, and hospitals whose development in California has been quickened by the Affordable Care Act, carry with them both promise and pitfalls. On the positive side of the ledger, ACOs may improve the quality of medical care even as they lower the costs of that care. On the negative side of the ledger, ACOs may lead to a gain in market power for their participations, allowing those participants to increase the prices they charge to commercial insurers. It is thus a key question for antitrust enforcers to figure out how to separate the sheep from the goats. This article, representing our personal views as state antitrust enforcers in the California attorney general's office, offers our reflection on a number of ACO articles and studies in this special issue through the prism of this key question and sets out a number of additional issues that we believe warrant study in conjunction with ACOs.

  12. Regulation of Propylene and 1-Methylcyclopropene on Expressions of ACS and ACO Genes in Persimmon Fruits

    Institute of Scientific and Technical Information of China (English)

    LIU Le; RAO Jing-ping; CHANG Xiao-xiao; YI Shun-chao

    2009-01-01

    The regulation of postharvest treatment with propylene and 1-MCP on ethylene release rate and expressions of 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes in Fuping Janshi persimmon (Diospyros kaki L.) fruit were investigated. Fruits were treated with propylene and 1-MCP, then stored at 20℃, ethylene release rate of the treated fruits was measured at regular intervals and RNA was extracted for Northern blotting analysis. The results suggested that treatment with propylene accelerated the expressions of ACS and ACO genes and then enhanced the ethylene biosynthesis, while treatment with 1-MCP inhibited the expressions of two genes and their ethylene biosynthesis. Furthermore, different effects on expressions caused by treatments with propylene and 1-MCP existed in various fruit tissues, the inhibitory effect on ACS and ACO genes by 1-MCP was the strongest in pericarp, followed by pulp and core tissues, in the area near fruit stalk, the inhibitory effect was the weakest. While the enhanced effect on ACS and ACO genes by propylene increased from pulp, core, and pericarp to the area near fruit stalk. Expression of each member of ACS and ACO families in various tissues was also completely different, in control and propylene treatment, DKACS3 gene just expressed in the area near fruit stalk and did not express in other tissues, but DKACS2 gene expressed in four tissues by treatment with propylene.

  13. APIs and Researchers: The Emperor's New Clothes?

    Directory of Open Access Journals (Sweden)

    Jennifer Edmond

    2015-02-01

    Full Text Available As part of the Europeana Cloud (eCloud project, Trinity College Dublin investigated best practice in the use of web services, such as APIs, for accessing large data sets from cultural heritage collections. This research looked into the provision and use of APIs, and moreover, whether or not more customised programmatic access to datasets is what researchers want or need. In order to understand whether current patterns of API usage reflect a skills gap on the part of researchers or a mismatch of tool to purpose, we looked not only at the creators and developer/users of APIs, but also at humanists already re-using big data; approaches in cultural heritage institutions and other research infrastructures to bring API use to non-technical audiences; and the kinds of training and other support services available or emerging within the data-intensive humanities research lifecycle. We conducted both desk research and a series of 11 interviews with figures working as researchers, developers or data providers, including figures from both the API development and the data usage communities. This research, conducted under the eCloud project and supported by the European Commission’s ICT Policy and Support Programme (Grant number 325091, was begun in March 2014 and is now in its concluding validation stage. The results of the research are not yet finalised, but the contribution is already emerging of this work to the debate about APIs being either the way forward for digital cultural heritage collections, or the Emperor’s New Clothes (or maybe a bit of both.

  14. Comparative Foraging Behavior of Apis Cerana F. and Apis Mellifera L. in Rapeseed under Cage Condition in Chitwan, Nepal

    Directory of Open Access Journals (Sweden)

    Rameshwor Pudasaini

    2014-12-01

    Full Text Available An experiment was conducted to determine the foraging behavior of Apis mellifera L. and Apis cerana F. in rapeseed under cage condition in Chitwan, Nepal during 2012-2013. This experiment showed that Apis cerana F. foraged extra 42 minute per day as compared to Apis mellifera L. Apis cerana F. were more attracted to nectar, whereas Apis mellifera L. were more attracted to pollen collection throughout the day. The activities, in into hives and out from hives, for both species were recorded more at 2:00 pm and least at 8:00 am. The highest in-out were observed at 2:00 pm on both species as Apis mellifera L. 44.33 bees entered into hives and 49.66 bees went out of hives, whereas lower number of Apis cerana F. 43.66 bees entered into hives and 48.16 bees were out of hives. Apis mellifera L. collect 1.22:1 and 0.41:1 pollen nectar ratio at 10:00 am and 4:00 am whereas at same hours Apis cerana collect 1.16:1 and 0.30:1 pollen nectar ratio. Apis cerana F. foraged significantly higher number of rapeseed flowers and plants as compared to Apis mellifera L. under caged condition. It shows that Apis cerana F. was more efficient pollinator as compared to Apis mellifera L. under caged condition.

  15. Application of ACO-BP Algorithm to Fault Diagnosis in Chemical Process%ACO-BP算法在化工过程故障诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    陈剑雪

    2012-01-01

    将蚁群算法和BP神经网络相结合,利用蚁群优化算法与误差反向传播算法结合而构成的混合算法(ACO-BP)训练神经网络的权值和阈值,给出ACO-BP算法训练神经网络的基本原理和方法步骤,并将该算法应用于连续搅拌釜式反应器的故障诊断.仿真结果表明:ACO-BP算法具有较高的诊断精度,能够及时、有效地检测连续搅拌釜式反应器中存在的故障.%ACO-BP algorithm which combining ant colony optimization (ACO) algorithm with back-propagation (BP) algorithm was proposed to train neural network weights and thresholds; and both basic theory and steps of ACO-BP algorithm were given and the ACO-BP algorithm was applied to fault diagnosis of the continuous stirred-tank reactor (CSTR). Experimental results show that the ACO-BP neural network with high precision in fault diagnosis can detect the fault in CSTR promptly and effectively.

  16. Transplante cardíaco em amiloidose primária

    OpenAIRE

    Baumgratz,José Francisco; Vila,José Henrique Andrade; Guilhen,Claudia Jesus; Fonseca,Luciana da; Leite, Weverton Ferreira; D'andretta, Carlos; Tângari Junior, Américo; Silva,José Pedro da

    2009-01-01

    A amiloidose cardíaca é doença altamente limitante da sobrevida, por morte súbita na maioria dos pacientes. Pela agressão contra outros órgãos, particularmente rins e sistema nervoso central, o transplante cardíaco tem sido opção questionável, face à escassez de órgãos. O objetivo é relatar a evolução, com sobrevivência de 7 anos, da paciente após transplante cardíaco por amiloidose, em boas condições. Um ano após o transplante cardíaco, houve indicação de transplante renal, também pela agres...

  17. ACO-Initialized Wavelet Neural Network for Vibration Fault Diagnosis of Hydroturbine Generating Unit

    Directory of Open Access Journals (Sweden)

    Zhihuai Xiao

    2015-01-01

    Full Text Available Considering the drawbacks of traditional wavelet neural network, such as low convergence speed and high sensitivity to initial parameters, an ant colony optimization- (ACO- initialized wavelet neural network is proposed in this paper for vibration fault diagnosis of a hydroturbine generating unit. In this method, parameters of the wavelet neural network are initialized by the ACO algorithm, and then the wavelet neural network is trained by the gradient descent algorithm. Amplitudes of the frequency components of the hydroturbine generating unit vibration signals are used as feature vectors for wavelet neural network training to realize mapping relationship from vibration features to fault types. A real vibration fault diagnosis case result of a hydroturbine generating unit shows that the proposed method has faster convergence speed and stronger generalization ability than the traditional wavelet neural network and ACO wavelet neural network. Thus it can provide an effective solution for online vibration fault diagnosis of a hydroturbine generating unit.

  18. Asthma-chronic obstructive pulmonary disease overlap syndrome (ACOS): current literature review

    Science.gov (United States)

    Papaiwannou, Antonis; Porpodis, Konstantinos; Spyratos, Dionysios; Kioumis, Ioannis; Pitsiou, Georgia; Pataka, Athanasia; Tsakiridis, Kosmas; Arikas, Stamatis; Mpakas, Andreas; Tsiouda, Theodora; Katsikogiannis, Nikolaos; Kougioumtzi, Ioanna; Machairiotis, Nikolaos; Siminelakis, Stavros; Kolettas, Alexander; Kessis, George; Beleveslis, Thomas; Zarogoulidis, Konstantinos

    2014-01-01

    Asthma and chronic obstructive pulmonary disease (COPD) are chronic diseases, very common in general population. These obstructive airway illnesses are manifested with chronic inflammation affecting the whole respiratory tract. Obstruction is usually intermittent and reversible in asthma, but is progressive and irreversible in COPD. Asthma and COPD may overlap and converge, especially in older people [overlap syndrome—asthma-chronic obstructive pulmonary disease overlap syndrome (ACOS)]. Although ACOS accounts approximately 15-25% of the obstructive airway diseases, is not well recognised because of the structure of clinical trials. COPD studies exclude asthma patients and asthma studies exclude COPD patients, respectively. It is crucial to define asthma, COPD and overlap syndrome (ACOS), as notable clinical entities, which they share common pathologic and functional features, but they are characterized from differences in lung function, acute exacerbations, quality of life, hospital impact and mortality. PMID:24672688

  19. Effect of slow plastic and elastic straining on sulphide stress cracking and hydrogen embrittlement of 3. 5% Ni steel and APL 5L X60 pipeline steel

    Energy Technology Data Exchange (ETDEWEB)

    Erlings, J.G.; Groot, H.W. de; Nauta, J.

    1987-01-01

    A procedure is presented with which the roles of elastic and elastic-plastic straining in stress corrosion cracking (SCC) and hydrogen embrittlement (HE) can be determined. Premature failure of 3.5% Ni steels in sour and sweet environments due to SCC was only found when slow plastic straining was applied. With purely elastic slow straining the material remained crack-free, even in a buffered NACE solution. Depending on the sourness of the environment, the API 5L X60 pipeline material did not always need plastic straining to suffer HE cracking. Under none of the test conditions studied was hardened material susceptible to SCC or HE cracking. The non-hardened material tested was not susceptible to SCC in the various CO/sub 2/- and/or H/sub 2/S-containing media used.

  20. Trauma cardíaco penetrante – relato de caso

    Directory of Open Access Journals (Sweden)

    Caio Augusto Perete

    2014-10-01

    Full Text Available Introdução: O trauma cardíaco penetrante (TCP, embora raro, é uma condição grave e potencialmente letal. Estudos mostram que 60 a 80% dessas vítimas evoluem a óbito no local do acidente ou no trajeto para a unidade de emergência (UE. Ferimentos na área de Ziegler exigem maior atenção em função do risco de lesão cardíaca (LC. Objetivo: Relatar o caso de ferimento por arma branca (FAB em zona de Ziedler com lesão transfixante de artéria descendente anterior (ADA. Método: Observação clínica e cirúrgica, registro de imagem e revisão da literatura. Relato de caso: Paciente masculino, 23 anos, admitido na UE 4 horas após sofrer FAB em 5o intercosto com linha hemiclavicular esquerda. Sedado, com intubação orotraqueal e ventilação mecânica, murmúrio vesicular presente à direita e diminuído em hemitórax esquerdo (com drenagem de 800 mL de sangue, choque hipovolêmico grau III, bulhas cardíacas abafadas e turgência jugular. Após janela pericárdica positiva optou-se por esternotomia, evidenciando perfuração de 0,5cm em septo interventricular com sangramento ativo da região distal da ADA. Foi realizada sutura simples da LC. No pós-operatório (PO imediato, o eletrocardiograma apresentou supradesnivelamento do segmento ST nas derivações V2, V3 e V4 e troponina positiva que, ao longo dos dias, apresentaram normalidade, recebendo alta hospitalar no 6o dia PO. Conclusões: Os TCP ainda são um desafio para os cirurgiões. Ferimentos na área de Ziedler devem pressupor LC já que alguns pacientes são inicialmente assintomáticos, exigindo para seu diagnóstico alto índice de suspeita.

  1. CALIBRATION, OPTIMIZATION, AND SENSITIVITY AND UNCERTAINTY ALGORITHMS APPLICATION PROGRAMMING INTERFACE (COSU-API)

    Science.gov (United States)

    The Application Programming Interface (API) for Uncertainty Analysis, Sensitivity Analysis, and Parameter Estimation (UA/SA/PE API) tool development, here fore referred to as the Calibration, Optimization, and Sensitivity and Uncertainty Algorithms API (COSU-API), was initially d...

  2. Advanced API security securing APIs with OAuth 2.0, OpenID Connect, JWS, and JWE

    CERN Document Server

    Siriwardena, Prabath

    2014-01-01

    Advanced API Security is a complete reference to the next wave of challenges in enterprise security--securing public and private APIs. API adoption in both consumer and enterprises has gone beyond predictions. It has become the 'coolest' way of exposing business functionalities to the outside world. Both your public and private APIs, need to be protected, monitored and managed. Security is not an afterthought, but API security has evolved a lot in last five years. The growth of standards, out there, has been exponential. That's where AdvancedAPI Security comes in--to wade through the weeds

  3. Comparative virulence and competition between Nosema apis and Nosema ceranae in honey bees (Apis mellifera).

    Science.gov (United States)

    Milbrath, Meghan O; van Tran, Toan; Huang, Wei-Fong; Solter, Leellen F; Tarpy, David R; Lawrence, Frank; Huang, Zachary Y

    2015-02-01

    Honey bees (Apis mellifera) are infected by two species of microsporidia: Nosema apis and Nosemaceranae. Epidemiological evidence indicates that N. ceranae may be replacing N. apis globally in A. mellifera populations, suggesting a potential competitive advantage of N. ceranae. Mixed infections of the two species occur, and little is known about the interactions among the host and the two pathogens that have allowed N. ceranae to become dominant in most geographical areas. We demonstrated that mixed Nosema species infections negatively affected honey bee survival (median survival=15-17days) more than single species infections (median survival=21days and 20days for N. apis and N. ceranae, respectively), with median survival of control bees of 27days. We found similar rates of infection (percentage of bees with active infections after inoculation) for both species in mixed infections, with N. apis having a slightly higher rate (91% compared to 86% for N. ceranae). We observed slightly higher spore counts in bees infected with N. ceranae than in bees infected with N. apis in single microsporidia infections, especially at the midpoint of infection (day 10). Bees with mixed infections of both species had higher spore counts than bees with single infections, but spore counts in mixed infections were highly variable. We did not see a competitive advantage for N. ceranae in mixed infections; N. apis spore counts were either higher or counts were similar for both species and more N. apis spores were produced in 62% of bees inoculated with equal dosages of the two microsporidian species. N. ceranae does not, therefore, appear to have a strong within-host advantage for either infectivity or spore growth, suggesting that direct competition in these worker bee mid-guts is not responsible for its apparent replacement of N. apis.

  4. Unified Medical Language System Terminology Services (UTS) API

    Data.gov (United States)

    U.S. Department of Health & Human Services — The UTS API is intended for application developers to perform Web service calls and retrieve UMLS data within their own applications. The UTS API provides the...

  5. MODAPS Web Services API for LANCE-MODIS

    Data.gov (United States)

    National Aeronautics and Space Administration — MODAPS Web Services API for LANCE-MODIS. This Application Programming Interface (API) encompasses a library of functions that provides programmatic data access to...

  6. Comparing Speech Recognition Systems (Microsoft API, Google API And CMU Sphinx

    Directory of Open Access Journals (Sweden)

    Veton Këpuska

    2017-03-01

    Full Text Available The idea of this paper is to design a tool that will be used to test and compare commercial speech recognition systems, such as Microsoft Speech API and Google Speech API, with open-source speech recognition systems such as Sphinx-4. The best way to compare automatic speech recognition systems in different environments is by using some audio recordings that were selected from different sources and calculating the word error rate (WER. Although the WER of the three aforementioned systems were acceptable, it was observed that the Google API is superior.

  7. Pro REST API development with nodejs

    CERN Document Server

    Doglio, Fernando

    2015-01-01

    Pro REST API Development with Node.js is your guide to managing and understanding the full capabilities of successful REST development. API design is a hot topic in the programming world, but not many resources exist for developers to really understand how you can leverage the advantages. This book will provide a brief background on REST and the tools it provides (well known and not so well known). Understand how there is more to REST than just JSON and URLs. You will then cover and compare the maintained modules currently available in the npm community, including Express, Restify, Vatican,

  8. 47 CFR 61.46 - Adjustments to the API.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Adjustments to the API. 61.46 Section 61.46... Rules for Dominant Carriers § 61.46 Adjustments to the API. (a) Except as provided in paragraphs (d) and... carrier must calculate an API for each affected basket pursuant to the following methodology: APIt =...

  9. Effect of natural aging on the microstructural regions, mechanical properties, corrosion resistance and fracture in welded joints on API5L X52 steel pipeline

    Energy Technology Data Exchange (ETDEWEB)

    Vargas-Arista, B.; Albiter, A.; Garcia-Vazquez, F.; Mendoza-Camargo, O.; Hallen, J. M.

    2014-07-01

    A characterization study was done to analyze how microstructural regions affect the mechanical properties, corrosion and fractography of the Heat Affected Zone (HAZ), weld bead and base metal for pipe naturally aged for 21 years at 30 degree centigrade. Results showed that microstructures exhibited damage and consequently decrease in properties, resulting in over-aged due to service. SEM analysis showed that base metal presented coarse ferrite grain. Tensile test indicated that microstructures showed discontinuous yield. Higher tensile strength was obtained for weld bead, which exhibited a lower impact energy in comparison to that of HAZ and base metal associated with brittle fracture by trans-granular cleavage. The degradation of properties was associated with the coarsening of nano-carbides observed through TEM images analysis, which was confirmed by SEM fractography of tensile and impact fracture surfaces. The weld bead reached the largest void density and highest susceptibility to corrosion in H{sub 2}S media when compared to those of the HAZ and base metal. (Author)

  10. Effect of natural aging on the microstructural regions, mechanical properties, corrosion resistance and fracture in welded joints on API5L X52 steel pipeline

    Directory of Open Access Journals (Sweden)

    Vargas-Arista, Benjamín

    2014-09-01

    Full Text Available A characterization study was done to analyze how microstructural regions affect the mechanical properties, corrosion and fractography of the Heat Affected Zone (HAZ, weld bead and base metal for pipe naturally aged for 21 years at 30 °C. Results showed that microstructures exhibited damage and consequently decrease in properties, resulting in over-aged due to service. SEM analysis showed that base metal presented coarse ferrite grain. Tensile test indicated that microstructures showed discontinuous yield. Higher tensile strength was obtained for weld bead, which exhibited a lower impact energy in comparison to that of HAZ and base metal associated with brittle fracture by trans-granular cleavage. The degradation of properties was associated with the coarsening of nano-carbides observed through TEM images analysis, which was confirmed by SEM fractography of tensile and impact fracture surfaces. The weld bead reached the largest void density and highest susceptibility to corrosion in H2S media when compared to those of the HAZ and base metal.Se realizó un estudio de caracterización para analizar cómo la microestructura afecta a las propiedades mecánicas, corrosión y fractura de la zona afectada por calor (ZAC, soldadura y metal base para tubería envejecida naturalmente durante 21 años a 30 °C. Los resultados indicaron que las microestructuras presentaron daño y consecuentemente reducción en propiedades mecánicas, como consecuencia del envejecimiento por servicio. El estudio mediante MEB mostró que el metal base presenta grano ferrítico grueso. La prueba de tensión indicó que las microestructuras mostraron fluencia discontinua. La mayor resistencia a la tracción se presentó en la soldadura, la cual alcanzó menor energía de impacto en comparación con la ZAC y metal base asociado con fractura frágil por clivaje transgranular. La degradación de las propriedades está en relación con el engrosamiento de nanocarburos observados a través del análisis de imágenes por MET, lo que se confirmó mediante fractografía por MEB de superfícies de fractura bajo tensión e impacto. La soldadura alcanzó la mayor densidad de microhuecos y la mayor susceptibilidad a la corrosión en un medio conteniendo H2S que cuando se compara con la ZAC y metal base.

  11. Desenvolvimento de ensaios de corrosão-fadiga para juntas soldadas de aço API 5L X60

    OpenAIRE

    Fabrício Pinheiro dos Santos

    2006-01-01

    Este trabalho tem por objetivo desenvolver um equipamento de ensaios para avaliar a vida de juntas soldadas, provenientes de tubulações para extração de petróleo, submetidas à corrosão-fadiga. Para atingir este objetivo foi estudado em que condições de corrosãofadiga uma tubulação esta exposta em alto mar sendo então definidos os parâmetros mais relevantes para serem reproduzidos em laboratório em corpos de prova menores. Nesta etapa foram definidos quais seriam os parâmetros de ensaio que o ...

  12. Observations of damage modes as a function of microstructure during NACE TM0177-96 tensile testing of API 5L grade X60 linepipe steels

    Energy Technology Data Exchange (ETDEWEB)

    Gingell, A.D.B.; Garat, X. [IRSID (France)

    1999-11-01

    The mechanisms of sulfide stress cracking (SSC) of grade X60 Iinepipe steels produced by thermomechanical controlled processing (TMCP) have been investigated by applying acoustic emission testing to the NACE TM0177-96 method A tensile test. This method enables the interruption of the test before failure, allowing the observation of the development of subcritical damage as a function of microstructure. The results are interpreted in terms of a correlation between local failure mode and microstructural features, with cracking obeying the kinetics described by acoustic emission. Apart from the successful application of acoustic emission testing to the NACE TM0177-96 method A tensile test, this test method has also been shown to reveal Stress-Oriented Hydrogen Induced Cracking (SOHIC) in susceptible microstructure.

  13. Postharvest Analysis of Lowland Transgenic Tomato Fruits Harboring hpRNAi-ACO1 Construct

    Directory of Open Access Journals (Sweden)

    Bita Behboodian

    2012-01-01

    Full Text Available The plant hormone, ethylene, is an important regulator which involved in regulating fruit ripening and flower senescence. In this study, RNA interference (RNAi technology was employed to silence the genes involved in ethylene biosynthetic pathway. This was achieved by blocking the expression of specific gene encoding the ACC oxidase. Initially, cDNA corresponding to ACO1 of lowland tomato cultivar (MT1, which has high identity with ACO1 of Solanum lycopersicum in GenBank, was cloned through RT-PCR. Using a partial coding region of ACO1, one hpRNAi transformation vector was constructed and expressed ectopically under the 35S promoter. Results showed that transgenic lines harboring the hpRNA-ACO1 construct had lower ethylene production and a longer shelf life of 32 days as compared to 10 days for wild-type fruits. Changes in cell wall degrading enzyme activities were also investigated in cases where the transgenic fruits exhibited reduced rates of firmness loss, which can be associated with a decrease in pectin methylesterase (PME and polygalacturonase (PG activities. However, no significant change was detected in both transgenic and wild-type fruits in terms of β-galactosidase (β-Gal activity and levels of total soluble solid, titratable acid and ascorbic acid.

  14. Post-Acute Care and ACOs — Who Will Be Accountable?

    Science.gov (United States)

    McWilliams, J Michael; Chernew, Michael E; Zaslavsky, Alan M; Landon, Bruce E

    2013-01-01

    Objective To determine how the inclusion of post-acute evaluation and management (E&M) services as primary care affects assignment of Medicare beneficiaries to accountable care organizations (ACOs). Data Sources Medicare claims for a random 5 percent sample of 2009 Medicare beneficiaries linked to American Medical Association Group Practice data identifying provider groups sufficiently large to be eligible for ACO program participation. Study Design We calculated the fraction of community-dwelling beneficiaries whose assignment shifted, as a consequence of including post-acute E&M services, from the group providing their outpatient primary care to a different group providing their inpatient post-acute care. Principal Findings Assignment shifts occurred for 27.6 percent of 25,992 community-dwelling beneficiaries with at least one post-acute skilled nursing facility stay, and they were more common for those incurring higher Medicare spending. Those whose assignment shifted constituted only 1.3 percent of all community-dwelling beneficiaries cared for by large ACO-eligible organizations (n = 535,138), but they accounted for 8.4 percent of total Medicare spending for this population. Conclusions Under current Medicare assignment rules, ACOs may not be accountable for an influential group of post-acute patients, suggesting missed opportunities to improve care coordination and reduce inappropriate readmissions. PMID:23350910

  15. Standard methods for Apis mellifera propolis research

    Science.gov (United States)

    Propolis is one of the most fascinating honey bee (Apis mellifera L.) products. It is a plant derived product that bees produce from resins that they collect from different plant organs and with which they mix beeswax. Propolis is a building material and a protective agent in the beehive. It also pl...

  16. The Apis mellifera filamentous virus genome

    Science.gov (United States)

    A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double strand DNA molecule of approximately 498’500 nucleotides with a GC content of 50.8%. It encompasses 251 non overlapping open reading frames (ORFs), e...

  17. Integrated platform and API for electrophysiological data.

    Science.gov (United States)

    Sobolev, Andrey; Stoewer, Adrian; Leonhardt, Aljoscha; Rautenberg, Philipp L; Kellner, Christian J; Garbers, Christian; Wachtler, Thomas

    2014-01-01

    Recent advancements in technology and methodology have led to growing amounts of increasingly complex neuroscience data recorded from various species, modalities, and levels of study. The rapid data growth has made efficient data access and flexible, machine-readable data annotation a crucial requisite for neuroscientists. Clear and consistent annotation and organization of data is not only an important ingredient for reproducibility of results and re-use of data, but also essential for collaborative research and data sharing. In particular, efficient data management and interoperability requires a unified approach that integrates data and metadata and provides a common way of accessing this information. In this paper we describe GNData, a data management platform for neurophysiological data. GNData provides a storage system based on a data representation that is suitable to organize data and metadata from any electrophysiological experiment, with a functionality exposed via a common application programming interface (API). Data representation and API structure are compatible with existing approaches for data and metadata representation in neurophysiology. The API implementation is based on the Representational State Transfer (REST) pattern, which enables data access integration in software applications and facilitates the development of tools that communicate with the service. Client libraries that interact with the API provide direct data access from computing environments like Matlab or Python, enabling integration of data management into the scientist's experimental or analysis routines.

  18. DySectAPI: Scalable Prescriptive Debugging

    DEFF Research Database (Denmark)

    Jensen, Nicklas Bo; Karlsson, Sven; Quarfot Nielsen, Niklas;

    We present the DySectAPI, a tool that allow users to construct probe trees for automatic, event-driven debugging at scale. The traditional, interactive debugging model, whereby users manually step through and inspect their application, does not scale well even for current supercomputers. While...

  19. How Do Developers Use APIs? A Case Study in Concurrency

    DEFF Research Database (Denmark)

    Blom, Stefan; Kiniry, Joseph; Huisman, Marieke

    2013-01-01

    With the omnipresent usage of APIs in software development, it has become important to analyse how the routines and functionalities of APIs are actually used. This information is in particular useful for API developers, to make decisions about future updates of the API. However, also for developers...... of static analysis and verification tools this information is highly important, because it indicates where and how to put the most efficient effort in annotating APIs, to make them usable for the static analysis and verification tools. This paper presents an analysis of the usage of the routines...

  20. An Implementation Model of Parlay MMCCS API Based on SIP

    Institute of Scientific and Technical Information of China (English)

    GUAN You-qing; SHEN Su-bin

    2006-01-01

    Parlay Multi Media Call Control Services(MMCCS) Application Programming Interfaces (API) based on Session Initiation Protocol (SIP) is essential for the implementation of Parlay Call Control (CC) API. This paper first proposes an implementation model of Parlay MMCCS API based on SIP, then presents a mapping between SIP and MMCCS API as far as methods and parameters are concerned, illustrates corresponding relationships between different components of SIP and MMCCS API by means of an application collaboration diagram, and finally presents an application using Java codes as well as some SIP messages. The application shows that a majority of MMCCS API based on SIP can be implemented and therefore verifies our mapping between MMCCS API and SIP.

  1. Hydrogen sulfide corrosion of weld regions in API X52 steel; Corrosion por acido sulfhidrico de las regiones de soldadura en acero API X52

    Energy Technology Data Exchange (ETDEWEB)

    Arenas-Martinez, L.F [Universidad Autonoma de Coahuila, Coahuila (Mexico)]. E-mail: fernando.arenas@uadec.edu.mx; Garcia-Cerecero, G. [Corporacion Mexicana de Investigacion en Materiales S.A. de C.V., Saltillo, Coahuila (Mexico)]. E-mail: ggarcia@comimsa.com

    2012-10-15

    The corrosion behavior of gas metal arc welding (GMAW) regions has been studied using potentiodynamic polarization and polarization resistance (LPR) techniques. Experiments were conducted in hydrogen sulfide (H{sub 2}S)-containing brine and in H{sub 2}S-free brine. Welds were made on API 5L X52 steel. Due to differences in their microstructure, chemical composition and residual stress level, weld regions exhibited different responses under H{sub 2}S corrosion. Base metal exhibited the highest corrosion rate (CR) and the most cathodic corrosion potential. [Spanish] Se estudio el comportamiento ante la corrosion de las regiones de soldadura de un cordon realizado por arco metalico con gas (GMAW) sobre un acero grado API X52 mediante las tecnicas de polarizacion potencio dinamica y resistencia a la polarizacion (LPR). Los experimentos se realizaron utilizando salmuera con 300 ppm de acido sulfhidrico (H{sub 2}S) y salmuera libre de H{sub 2}S como electrolitos. Debido a las diferencias en su microestructura, composicion quimica y el nivel de esfuerzos residuales, las regiones de soldadura mostraron diferentes respuestas a la corrosion por H{sub 2}S. El metal base exhibio la velocidad de corrosion (VC) mas alta y el potencial de corrosion mas catodico.

  2. Population Genetics of Nosema apis and Nosema ceranae: One Host (Apis mellifera) and Two Different Histories.

    Science.gov (United States)

    Maside, Xulio; Gómez-Moracho, Tamara; Jara, Laura; Martín-Hernández, Raquel; De la Rúa, Pilar; Higes, Mariano; Bartolomé, Carolina

    2015-01-01

    Two microsporidians are known to infect honey bees: Nosema apis and Nosema ceranae. Whereas population genetics data for the latter have been released in the last few years, such information is still missing for N. apis. Here we analyze the patterns of nucleotide polymorphism at three single-copy loci (PTP2, PTP3 and RPB1) in a collection of Apis mellifera isolates from all over the world, naturally infected either with N. apis (N = 22) or N. ceranae (N = 23), to provide new insights into the genetic diversity, demography and evolution of N. apis, as well as to compare them with evidence from N. ceranae. Neutral variation in N. apis and N. ceranae is of the order of 1%. This amount of diversity suggests that there is no substantial differentiation between the genetic content of the two nuclei present in these parasites, and evidence for genetic recombination provides a putative mechanism for the flow of genetic information between chromosomes. The analysis of the frequency spectrum of neutral variants reveals a significant surplus of low frequency variants, particularly in N. ceranae, and suggests that the populations of the two pathogens are not in mutation-drift equilibrium and that they have experienced a population expansion. Most of the variation in both species occurs within honey bee colonies (between 62%-90% of the total genetic variance), although in N. apis there is evidence for differentiation between parasites isolated from distinct A. mellifera lineages (20%-34% of the total variance), specifically between those collected from lineages A and C (or M). This scenario is consistent with a long-term host-parasite relationship and contrasts with the lack of differentiation observed among host-lineages in N. ceranae (mellifera worldwide population is a recent event.

  3. A Hybrid Algorithm Based on ACO and PSO for Capacitated Vehicle Routing Problems

    Directory of Open Access Journals (Sweden)

    Yucheng Kao

    2012-01-01

    Full Text Available The vehicle routing problem (VRP is a well-known combinatorial optimization problem. It has been studied for several decades because finding effective vehicle routes is an important issue of logistic management. This paper proposes a new hybrid algorithm based on two main swarm intelligence (SI approaches, ant colony optimization (ACO and particle swarm optimization (PSO, for solving capacitated vehicle routing problems (CVRPs. In the proposed algorithm, each artificial ant, like a particle in PSO, is allowed to memorize the best solution ever found. After solution construction, only elite ants can update pheromone according to their own best-so-far solutions. Moreover, a pheromone disturbance method is embedded into the ACO framework to overcome the problem of pheromone stagnation. Two sets of benchmark problems were selected to test the performance of the proposed algorithm. The computational results show that the proposed algorithm performs well in comparison with existing swarm intelligence approaches.

  4. New data association technique based on ACO with directional information considered

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Due to the advantages of ant colony optimization (ACO) in solving complex problems, a new data association algorithm based on ACO in a cluttered environment called DACDA is proposed. In the proposed method, the concept for tour and the length of tour are redefined. Additionally, the directional information is incorporated into the proposed method because it is one of the most important factors that affects the performance of data association. Kalraan filter is employed to estimate target states. Computer simulation results show that the proposed method could carry out data association in an acceptable CPU time, and the correct data association rate is higher than that obtained by the data association (DA) algorithm not combined with directional information.

  5. Trauma cardíaco penetrante. Experiencia en el Hospital Nacional Cayetano Heredia 1984-1993.

    OpenAIRE

    Castañeda, Enrique; BAZAN, Noé; VASQUEZ, Julio; DELGADO, Hugo

    2013-01-01

    Se revisaron las historias clínicas de 21 pacientes que sufrieron traumatismo cardíaco penetrante, atendidos entre enero de 1984 y diciembre de 1993 en el Hospital Nacional Cayetano Heredia de Lima – Perú. Todos los pacientes fueron de sexo masculino y correspondieron en el 76%, a la segunda y tercera década de la vida. La mayoría de traumatismos fueron por herida cortopunzante (85.7%) y la forma de presentación más frecuente fué taponamiento cardíaco (77.8%). El ventrículo derecho (47.6%) e ...

  6. Tailoring Systems Engineering Processes in a Conceptual Design Environment: A Case Study at NASA Marshall Spaceflight Center's ACO

    Science.gov (United States)

    Mulqueen, John; Maples, C. Dauphne; Fabisinski, Leo, III

    2012-01-01

    This paper provides an overview of Systems Engineering as it is applied in a conceptual design space systems department at the National Aeronautics and Space Administration (NASA) Marshall Spaceflight Center (MSFC) Advanced Concepts Office (ACO). Engineering work performed in the NASA MFSC's ACO is targeted toward the Exploratory Research and Concepts Development life cycle stages, as defined in the International Council on Systems Engineering (INCOSE) System Engineering Handbook. This paper addresses three ACO Systems Engineering tools that correspond to three INCOSE Technical Processes: Stakeholder Requirements Definition, Requirements Analysis, and Integration, as well as one Project Process Risk Management. These processes are used to facilitate, streamline, and manage systems engineering processes tailored for the earliest two life cycle stages, which is the environment in which ACO engineers work. The role of systems engineers and systems engineering as performed in ACO is explored in this paper. The need for tailoring Systems Engineering processes, tools, and products in the ever-changing engineering services ACO provides to its customers is addressed.

  7. HI deficiency in the galaxy cluster ACO 3627. ATCA observations in the Great Attractor region

    CERN Document Server

    Vollmer, B; Van Driel, W; Henning, P A; Kraan-Korteweg, R C; Balkowski, C; Woudt, P A; Duschl, W J

    2001-01-01

    ATCA 21 cm HI observations of the rich galaxy cluster ACO 3627 in the Great Attractor region are presented. Three fields of 30' diameter located within one Abell radius of ACO 3627 were observed with a resolution of 15'' and an rms noise of \\sim 1 mJy/beam. Only two galaxies were detected in these fields. We compare their HI distribution to new optical R-band images and discuss their velocity fields. The first galaxy is a gas-rich unperturbed spiral whereas the second shows a peculiar HI distribution. The estimated 3-sigma HI mass limit of our observations is \\sim 7 x 10^8 M_{\\odot} for a line width of 150 km s^{-1}. The non-detection of a considerable number of luminous spiral galaxies indicates that the spiral galaxies are HI deficient. The low detection rate is comparable to the HI deficient Coma cluster (Bravo-Alfaro et al. 2000). ACO 3627 is a bright X-ray cluster. We therefore suspect that ram pressure stripping is responsible for the HI deficiency of the bright cluster spirals.

  8. De la Locura de doble forma a la Psicosis maníaco-depresiva.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1995-01-01

    Full Text Available Sin entrar en polémicas sobre a quién atribuir la primera descripción de lo que actualmente llamamos Psicosis maníaco-depresiva, es decir, sin tomar partido por Baillarger o J.-P. Falret, lo cierto es que a mediados del pasado siglo los alienistas franceses orquestaron un saber nosológico en el que se reunían en una única categoría dos manifestaciones extremas del humor: la manía y la melancolía. Tras las descripciones fundacionales de Baillarger y de Falret, fechadas ambas en el mismo año, 1854, se abrió un espacio nosológico opuesto al terreno de los delirios crónicos: las psicosis del humor frente a las psicosis de la razón, o bien psicosis maníaco-depresiva frente a esquizofrenia-paranoia. De la última cosecha del nosógrafo por excelencia, Emil Kraepelin, proviene la descripción clínica más completa y matizada de la locura maníaco-depresiva (das manisch-depressive Irresein. Dos años después, en 1915, S. Freud mostró en Duelo y Melancolía la infraestructura metapsicológica de la manía y de la melancolía.

  9. APIs for QoS configuration in Software Defined Networks

    DEFF Research Database (Denmark)

    Caba, Cosmin Marius; Soler, José

    2015-01-01

    such as configuration of devices, ports, queues, etc. An Application Programming Interface (API) for dynamic configuration of QoS resources in the network devices is implemented herein, by using the capabilities of OVSDB. Further, the paper demonstrates the possibility to create network services with coarse granularity...... on top of the fine granular services exposed by the QoS configuration API at the SDNC. A series of tests emphasize the capabilities and the performance of the implemented QoS configuration API....

  10. Culture of human mesenchymal stem cells on microcarriers in a 5 l stirred-tank bioreactor.

    Science.gov (United States)

    Rafiq, Qasim A; Brosnan, Kathryn M; Coopman, Karen; Nienow, Alvin W; Hewitt, Christopher J

    2013-08-01

    For the first time, fully functional human mesenchymal stem cells (hMSCs) have been cultured at the litre-scale on microcarriers in a stirred-tank 5 l bioreactor, (2.5 l working volume) and were harvested via a potentially scalable detachment protocol that allowed for the successful detachment of hMSCs from the cell-microcarrier suspension. Over 12 days, the dissolved O2 concentration was >45 % of saturation and the pH between 7.2 and 6.7 giving a maximum cell density in the 5 l bioreactor of 1.7 × 10(5) cells/ml; this represents >sixfold expansion of the hMSCs, equivalent to that achievable from 65 fully-confluent T-175 flasks. During this time, the average specific O2 uptake of the cells in the 5 l bioreactor was 8.1 fmol/cell h and, in all cases, the 5 l bioreactors outperformed the equivalent 100 ml spinner-flasks run in parallel with respect to cell yields and growth rates. In addition, yield coefficients, specific growth rates and doubling times were calculated for all systems. Neither the upstream nor downstream bioprocessing unit operations had a discernible effect on cell quality with the harvested cells retaining their immunophenotypic markers, key morphological features and differentiation capacity.

  11. Privacy Issues of the W3C Geolocation API

    CERN Document Server

    Doty, Nick; Wilde, Erik

    2010-01-01

    The W3C's Geolocation API may rapidly standardize the transmission of location information on the Web, but, in dealing with such sensitive information, it also raises serious privacy concerns. We analyze the manner and extent to which the current W3C Geolocation API provides mechanisms to support privacy. We propose a privacy framework for the consideration of location information and use it to evaluate the W3C Geolocation API, both the specification and its use in the wild, and recommend some modifications to the API as a result of our analysis.

  12. Enfermedad inflamatoria intestinal en pacientes celíacos Inflammatory bowel disease in celiac patients

    Directory of Open Access Journals (Sweden)

    M. Masachs

    2007-08-01

    Full Text Available Introducción: se ha sugerido una potencial asociación entre la enfermedad celíaca y la enfermedad inflamatoria intestinal, que puede justificar que ambas enfermedades puedan presentarse en un mismo enfermo o en sus familiares de primer orden con mayor frecuencia de lo esperado. Objetivo: determinar la prevalencia de la enfermedad de Crohn y la colitis ulcerosa en los enfermos celíacos y en sus familiares. Método: estudio epidemiológico prospectivo transversal en un grupo de pacientes celíacos, sus familiares de primer grado y un grupo control de características epidemiológicas similares, constituido por familiares de pacientes que acuden al Servicio de Urgencias por un problema agudo. Para detectar la existencia de colitis ulcerosa y enfermedad de Crohn en los celíacos y sus familiares, se realizó una entrevista semiestructurada. Resultados: se han incluido 86 celíacos y 432 familiares, que se han comparado con 809 controles (129 pacientes con una enfermedad aguda y 680 familiares de primer grado suyos. Se han detectado 3 casos de enfermedad de Crohn en el grupo de los enfermos celíacos y 4 casos de enfermedad de Crohn en sus familiares. Sólo se ha detectado 1 caso de enfermedad de Crohn en el grupo control (p Introduction: a potential association between celic disease and inflammatory bowel disease hs been suggested, which may explain the fact that both disorders occasionally present in one patient or in his/her first-degree relatives more frequently than expected. Objective: to establish the prevalence of Crohn's disease and ulcerative colitis in celiac patients and their relatives. Method: a cross-sectional, prospective epidemiological study in a group of celiac patients, their first-degree relatives, and a control group with similar epidemiological characteristics including the relatives of patients presenting at the ER for acute conditions. A semistructured interview was used to identify the presence of Crohn's disease and

  13. Large scale validation of the M5L lung CAD on heterogeneous CT datasets

    Energy Technology Data Exchange (ETDEWEB)

    Lopez Torres, E., E-mail: Ernesto.Lopez.Torres@cern.ch, E-mail: cerello@to.infn.it [CEADEN, Havana 11300, Cuba and INFN, Sezione di Torino, Torino 10125 (Italy); Fiorina, E.; Pennazio, F.; Peroni, C. [Department of Physics, University of Torino, Torino 10125, Italy and INFN, Sezione di Torino, Torino 10125 (Italy); Saletta, M.; Cerello, P., E-mail: Ernesto.Lopez.Torres@cern.ch, E-mail: cerello@to.infn.it [INFN, Sezione di Torino, Torino 10125 (Italy); Camarlinghi, N.; Fantacci, M. E. [Department of Physics, University of Pisa, Pisa 56127, Italy and INFN, Sezione di Pisa, Pisa 56127 (Italy)

    2015-04-15

    Purpose: M5L, a fully automated computer-aided detection (CAD) system for the detection and segmentation of lung nodules in thoracic computed tomography (CT), is presented and validated on several image datasets. Methods: M5L is the combination of two independent subsystems, based on the Channeler Ant Model as a segmentation tool [lung channeler ant model (lungCAM)] and on the voxel-based neural approach. The lungCAM was upgraded with a scan equalization module and a new procedure to recover the nodules connected to other lung structures; its classification module, which makes use of a feed-forward neural network, is based of a small number of features (13), so as to minimize the risk of lacking generalization, which could be possible given the large difference between the size of the training and testing datasets, which contain 94 and 1019 CTs, respectively. The lungCAM (standalone) and M5L (combined) performance was extensively tested on 1043 CT scans from three independent datasets, including a detailed analysis of the full Lung Image Database Consortium/Image Database Resource Initiative database, which is not yet found in literature. Results: The lungCAM and M5L performance is consistent across the databases, with a sensitivity of about 70% and 80%, respectively, at eight false positive findings per scan, despite the variable annotation criteria and acquisition and reconstruction conditions. A reduced sensitivity is found for subtle nodules and ground glass opacities (GGO) structures. A comparison with other CAD systems is also presented. Conclusions: The M5L performance on a large and heterogeneous dataset is stable and satisfactory, although the development of a dedicated module for GGOs detection could further improve it, as well as an iterative optimization of the training procedure. The main aim of the present study was accomplished: M5L results do not deteriorate when increasing the dataset size, making it a candidate for supporting radiologists on large

  14. Adaptive runtime for a multiprocessing API

    Energy Technology Data Exchange (ETDEWEB)

    Antao, Samuel F.; Bertolli, Carlo; Eichenberger, Alexandre E.; O' Brien, John K.

    2016-10-11

    A computer-implemented method includes selecting a runtime for executing a program. The runtime includes a first combination of feature implementations, where each feature implementation implements a feature of an application programming interface (API). Execution of the program is monitored, and the execution uses the runtime. Monitor data is generated based on the monitoring. A second combination of feature implementations are selected, by a computer processor, where the selection is based at least in part on the monitor data. The runtime is modified by activating the second combination of feature implementations to replace the first combination of feature implementations.

  15. Adaptive runtime for a multiprocessing API

    Energy Technology Data Exchange (ETDEWEB)

    Antao, Samuel F.; Bertolli, Carlo; Eichenberger, Alexandre E.; O' Brien, John K.

    2016-11-15

    A computer-implemented method includes selecting a runtime for executing a program. The runtime includes a first combination of feature implementations, where each feature implementation implements a feature of an application programming interface (API). Execution of the program is monitored, and the execution uses the runtime. Monitor data is generated based on the monitoring. A second combination of feature implementations are selected, by a computer processor, where the selection is based at least in part on the monitor data. The runtime is modified by activating the second combination of feature implementations to replace the first combination of feature implementations.

  16. Rare royal families in honeybees, Apis mellifera

    Science.gov (United States)

    Moritz, Robin F. A.; Lattorff, H. Michael G.; Neumann, Peter; Kraus, F. Bernhard; Radloff, Sarah E.; Hepburn, H. Randall

    2005-10-01

    The queen is the dominant female in the honeybee colony, Apis mellifera, and controls reproduction. Queen larvae are selected by the workers and are fed a special diet (royal jelly), which determines caste. Because queens mate with many males a large number of subfamilies coexist in the colony. As a consequence, there is a considerable potential for conflict among the subfamilies over queen rearing. Here we show that honeybee queens are not reared at random but are preferentially reared from rare “royal” subfamilies, which have extremely low frequencies in the colony's worker force but a high frequency in the queens reared.

  17. grlc Makes GitHub Taste Like Linked Data APIs

    NARCIS (Netherlands)

    Meroño-Peñuela, A.; Hoekstra, Rinke; Sack, H; Rizzo, G; Steinmetz, N; Mladenić, D; Auer, S; Lange, C

    2016-01-01

    Building Web APIs on top of SPARQL endpoints is becoming a common practice to enable universal access to the integration favorable dataspace of Linked Data. However, the Linked Data community cannot expect users to learn SPARQL to query this dataspace, and Web APIs are the most extended way of enabl

  18. API and CPTDC Jointly Promote Chinese Enterprises to Go Abroad

    Institute of Scientific and Technical Information of China (English)

    Li Chunhui

    2005-01-01

    @@ In order to push the certification forward and help more Chinese enterprises to go abroad, API (American Petroleum Institute) and CPTDC (China Petroleum Technology & Development Corporation) jointly held a meeting between March 4 and 8, 2004, to promote API certification of Chinese enterprises.

  19. Interfacing RASCAL with Java APIs. Automated mapping with Apache POI

    NARCIS (Netherlands)

    Marmanidis, G.

    2013-01-01

    Interfacing RASCAL with Java APIs and reusing existing implementations is a required functionality in order to reduce effort and time in contrast with implementing a new one from scratch. In this study we are trying to interface RASCAL with Apache POI, a Java implemented API in order to import and a

  20. Experience API: Flexible, Decentralized and Activity-Centric Data Collection

    Science.gov (United States)

    Kevan, Jonathan M.; Ryan, Paul R.

    2016-01-01

    This emerging technology report describes the Experience API (xAPI), a new e-learning specification designed to support the learning community in standardizing and collecting both formal and informal distributed learning activities. Informed by Activity Theory, a framework aligned with constructivism, data is collected in the form of activity…

  1. Investigating an API for resilient exascale computing.

    Energy Technology Data Exchange (ETDEWEB)

    Stearley, Jon R.; Tomkins, James; VanDyke, John P.; Ferreira, Kurt Brian; Laros, James H.,; Bridges, Patrick

    2013-05-01

    Increased HPC capability comes with increased complexity, part counts, and fault occurrences. In- creasing the resilience of systems and applications to faults is a critical requirement facing the viability of exascale systems, as the overhead of traditional checkpoint/restart is projected to outweigh its bene ts due to fault rates outpacing I/O bandwidths. As faults occur and propagate throughout hardware and software layers, pervasive noti cation and handling mechanisms are necessary. This report describes an initial investigation of fault types and programming interfaces to mitigate them. Proof-of-concept APIs are presented for the frequent and important cases of memory errors and node failures, and a strategy proposed for lesystem failures. These involve changes to the operating system, runtime, I/O library, and application layers. While a single API for fault handling among hardware and OS and application system-wide remains elusive, the e ort increased our understanding of both the mountainous challenges and the promising trailheads. 3

  2. API E MIELE NEL MEDITERRANEO ANTICO

    Directory of Open Access Journals (Sweden)

    Matteo Bormetti

    2014-09-01

    Full Text Available L’importanza del miele come sostanza culturalmente ed economicamente rilevante nel mondo antico non è stata ancora del tutto indagata. Il miele e le api sono nondimeno presenze assidue e trasversali ai generi e alle tipologie di fonti antiche greche e romane, ed è pertanto da esse che una ricerca sull’argomento deve partire. I manufatti, le tecniche e i concetti descritti dagli antichi devono però essere relazionati con cautela al materiale archeologico rinvenuto, per fare ciò pare indispensabile un approccio che tenga conto delle nozioni biologiche, etologiche e zootecniche di base. Attraverso quest’ottica sono state ricapitolate le evidenze materiali provenienti dal bacino del Mediterraneo e concernenti gli strumenti e le strutture relative all’apicoltura, dalle arnie agli apiari, peril lungo arco cronologico che va dall’età del bronzo alla tarda antichità. Ciò è possibile per la tematica apicola grazie alla conservatività delle tecniche, strettamente legate ai severi dettami della natura fino alla rivoluzione ottocentesca delle arnie razionali a telai mobili del reverendo Langstroth. Sono inoltre analizzati gli utilizzi più frequenti del miele per come descritti dalle fonti letterarie nonché le implicazioni di api e miele nella ritualità e nel simbolismo della morte, del genere e della regalità, sebbene i risultati siano scarsi data la povertà dei riscontri archeologici e la limitata attenzione di cui questo argomento ha fin ora goduto.

  3. Coming 5th Edition of the API Standard 618: major changes compared to the API 618, 4th edition

    NARCIS (Netherlands)

    Pyle, A.; Eijk, A.; Elferink, H.

    2003-01-01

    This paper will present the highlights of changes that will be found in the 5th Edition of API 618, which is expected to be published in late 2003 or early 2004. Approximately every 5 years the API Standards are revised in such a way that the latest field experiences and proven designs are included

  4. Transplante cardíaco em amiloidose primária Heart transplantation in primary amyloidosis

    Directory of Open Access Journals (Sweden)

    José Francisco Baumgratz

    2009-09-01

    Full Text Available A amiloidose cardíaca é doença altamente limitante da sobrevida, por morte súbita na maioria dos pacientes. Pela agressão contra outros órgãos, particularmente rins e sistema nervoso central, o transplante cardíaco tem sido opção questionável, face à escassez de órgãos. O objetivo é relatar a evolução, com sobrevivência de 7 anos, da paciente após transplante cardíaco por amiloidose, em boas condições. Um ano após o transplante cardíaco, houve indicação de transplante renal, também pela agressão da doença. Esta paciente contrasta com outros três pacientes de nosso serviço que foram a óbito, ainda na fase de avaliação. Apesar de sua natureza multisistêmica, a amiloidose cardíaca pode, em pacientes selecionados, justificar o transplante cardíaco, pela gravidade do seu potencial evolutivo após o início dos sintomasCardiac amyloidosis is a disease with a gloom life expectance after the beginning of the symptomatic phase, usually with sudden death as the final event. The aggression to other organs, although, can make heart transplantation a disputable form of treatment taking into consideration the shortage of donor organs. The aim is to report the evolution with a survival of seven years after heart transplantation and in very fair condition of a patient with amyloidosis. One year after the heart transplantation, there was indication of renal transplantation also from the aggression from the disease. This patient compares' favorable with three other patients also from our service, who died early after de diagnosis. Even considering the multi systemic nature of amyloidosis, we can accept that in peculiar patients justified the heart transplantation, taking in the consideration the very bad prognosis of the disease

  5. Tumores cardíacos em criança: análise retrospectiva

    Directory of Open Access Journals (Sweden)

    Juliano Gomes Penha

    2013-02-01

    Full Text Available FUNDAMENTO: Avanço dos métodos não invasivos de imagem proporcionou o aumento no número de diagnóstico de tumores cardíacos. Apesar disso, a literatura apresenta poucos trabalhos envolvendo tumores cardíacos primários em crianças. OBJETIVO: Avaliar retrospectivamente casos de tumores cardíacos primários em crianças, considerando manifestações clínicas iniciais, exames utilizados para o diagnóstico, indicação cirúrgica, tipos histopatológicos encontrados e evolução pós-operatória imediata. MÉTODOS: O estudo foi retrospectivo, baseado na avaliação de prontuários no período de 1983 a 2011. Incluímos somente casos que foram orientados para tratamento cirúrgico no período. Avaliaram-se a idade na admissão, o diagnóstico pré-natal, a história familial, os sintomas iniciais e os resultados de exames realizados. Foram coletados, ainda, a data e indicação de cirurgia, os achados intraoperatórios, o resultado do exame histopatológico, assim como as complicações imediatas no pós-operatório. RESULTADOS: Dos 18 pacientes estudados, as manifestações clínicas mais encontradas foram dispneia e sopro cardíaco (7 e 6 pacientes, respectivamente; o método de complemento diagnóstico mais usado foi o ecocardiograma (18 pacientes; a obstrução cavitária ou do trato de entrada ou saída ventricular foi a principal indicação de cirurgia (12 casos; o perfil histológico mais encontrado foi rabdomioma (7 pacientes; a maioria dos pacientes apresentou boa evolução clínica. CONCLUSÃO: Neste estudo o diagnóstico por imagem foi basicamente ecocardiográfico, com boa correlação com os achados intraoperatórios. Os achados histopatológicos foram concordantes com a literatura, com o rabdomioma apresentando-se como o tumor mais comum em crianças. A evolução após tratamento cirúrgico mostrou-se favorável na maior parte dos casos.

  6. Taponamiento cardíaco por hipotiroidismo: descripción de 2 casos

    OpenAIRE

    José Rafael Rojas-Solano; María Claudia Peláez-Gil; Francisco Jiménez-Bolaños

    2005-01-01

    Rara vez el derrame pericárdico en el hipotiroidismo evoluciona al taponamiento cardíaco, complicación que es mortal si no se diagnostica y trata a tiempo. En este artículo describimos dos singulares casos y discutimos aspectos importantes de la fisiopatología, diagnóstico y manejo de esta enfermedad.Pericardial effusion in hypothyroidism seldom evolves to cardiac tamponade, a fatal complication if diagnosis and treatment are delayed. In this article, we describe 2 singular cases and discuss ...

  7. Taponamiento cardíaco secundario a carcinoma papilar esclerosante difuso de tiroides

    OpenAIRE

    2011-01-01

    El carcinoma papilar, variante esclerosante difusa, corresponde al 2% de todos los carcinomas papilares de la tiroides. Se caracteriza por comprometer de manera difusa y bilateral a la glándula tiroides. Clínicamente se manifiesta con metástasis ganglionares y pulmonares, afectando predominantemente a mujeres jóvenes. Se describe un caso de taponamiento cardíaco como presentación inicial de un carcinoma papilar de tiroides variante esclerosante difusa. Una mujer de 32 años concurrió al servic...

  8. Utilization of bee (Apis mellifera) honey for vinegar production at laboratory scale.

    Science.gov (United States)

    Ilha, E C; Sant'Anna, E; Torres, R C; Porto, A C; Meinert, E M

    2000-01-01

    Vinegar was obtained from bee (Apis mellifera) honey. The wort was prepared by diluting honey in distilled water to 21% total solids and by adding ammonium sulfate and ammonium phosphate. Saccharomyces cerevisiae was inoculated to the wort (4 g/L). Ethanol production was carried out at room temperature during 84 hours. In this study, 1 Kg of honey yielded about 5 L of wine, containing 8% alcohol (v/v), from a wort with 17.11% total sugars (w/v). The efficiency of the alcoholic fermentation was 81.34%. The acetic fermentation with an inoculum of mixed acetic microorganisms was performed by quick process in a 15 L vertical fermenter. This resulted in a vinegar containing up to 9% of acetic acid (w/v) and about 1% of alcohol (v/v). The acetic fermentation yielded between 91.24 and 97.21%. Approximately 5 L of honey vinegar with 9% acetic acid (w/v) were obtained from 1 Kg of bee honey. All attributes of honey vinegar showed acceptability index over 70%: 95.37% for appearance, 94.81% for color, 79.07% for odor and 75.56% for flavor, indicating it would show good consumer acceptability.

  9. Domainwise Web Page Optimization Based On Clustered Query Sessions Using Hybrid Of Trust And ACO For Effective Information Retrieval

    Directory of Open Access Journals (Sweden)

    Dr. Suruchi Chawla

    2015-08-01

    Full Text Available Abstract In this paper hybrid of Ant Colony OptimizationACO and trust has been used for domainwise web page optimization in clustered query sessions for effective Information retrieval. The trust of the web page identifies its degree of relevance in satisfying specific information need of the user. The trusted web pages when optimized using pheromone updates in ACO will identify the trusted colonies of web pages which will be relevant to users information need in a given domain. Hence in this paper the hybrid of Trust and ACO has been used on clustered query sessions for identifying more and more relevant number of documents in a given domain in order to better satisfy the information need of the user. Experiment was conducted on the data set of web query sessions to test the effectiveness of the proposed approach in selected three domains Academics Entertainment and Sports and the results confirm the improvement in the precision of search results.

  10. Comparison of learning and memory of Apis cerana and Apis mellifera.

    Science.gov (United States)

    Qin, Qiu-Hong; He, Xu-Jiang; Tian, Liu-Qing; Zhang, Shao-Wu; Zeng, Zhi-Jiang

    2012-10-01

    The honeybee is an excellent model organism for research on learning and memory among invertebrates. Learning and memory in honeybees has intrigued neuroscientists and entomologists in the last few decades, but attention has focused almost solely on the Western honeybee, Apis mellifera. In contrast, there have been few studies on learning and memory in the Eastern honeybee, Apis cerana. Here we report comparative behavioral data of color and grating learning and memory for A. cerana and A. mellifera in China, gathered using a Y-maze apparatus. We show for the first time that the learning and memory performance of A. cerana is significantly better on both color and grating patterns than that of A. mellifera. This study provides the first evidence of a learning and memory difference between A. cerana and A. mellifera under controlled conditions, and it is an important basis for the further study of the mechanism of learning and memory in honeybees.

  11. Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin

    Directory of Open Access Journals (Sweden)

    John A. Mawson

    2011-09-01

    Full Text Available The effects of Celluclast 1.5L concentration on the physicochemical characterization of gold kiwifruit pectin was evaluated. Varying the enzyme concentration affected the pectin yield and pectin physicochemical properties. The viscosity of extracted pectin was largely dependent on the enzyme concentration. Celluclast 1.5L with medium concentration exhibited the highest viscosity. Varying the enzyme concentration also influenced the molecular weight distribution. High molecular weight (Mw pectin (1.65 × 106 g/mol was obtained when the medium concentration was used. Overall, the study clearly reflects the importance of taking into consideration the amount of cellulytic enzyme added in order to determine the final quality of pectin.

  12. A novel phylogeny and morphological reconstruction of the PIN genes and first phylogeny of the ACC-oxidases (ACOs

    Directory of Open Access Journals (Sweden)

    Ronald Matthew Clouse

    2014-06-01

    Full Text Available The PIN and ACO gene families present interesting questions about the evolution of plant physiology, including testing hypotheses about the ecological drivers of their diversification and whether unrelated genes have been recruited for similar functions. The PIN-formed proteins contribute to the polar transport of auxin, a hormone which regulates plant growth and development. PIN loci are categorized into groups according to their protein length and structure, as well as subcellular localization. An interesting question with PIN genes is the nature of the ancestral form and location. ACOs are members of a superfamily of oxygenases and oxidases that catalyze the last step of ethylene synthesis, which regulates many aspects of the plant life cycle. We used publicly available PIN and ACO sequences to conduct phylogenetic analyses. Third codon positions of these genes in monocots have a high GC content, which could be historical but is more likely due to a mutational bias. Thus we developed methods to extract phylogenetic information from nucleotide sequences while avoiding this convergent feature. One method consisted in using only A-T transformations, and another used only the first and second codon positions for serine, which can only take A or T and G or C, respectively. We also conducted tree-searches for both gene families using unaligned amino acid sequences and dynamic homology. PIN genes appear to have diversified earlier than ACOs, with monocot and dicot copies more mixed in the phylogeny. However, gymnosperm PINs appear to be derived and not closely related to those from primitive plants. We find strong support for a long PIN gene ancestor with short forms subsequently evolving one or more times. ACO genes appear to have diversified mostly since the dicot-monocot split, as most genes cluster into a small number of monocot and dicot clades when the tree is rooted by genes from mosses. Gymnosperm ACOs were recovered as closely related and

  13. A novel phylogeny and morphological reconstruction of the PIN genes and first phylogeny of the ACC-oxidases (ACOs).

    Science.gov (United States)

    Clouse, Ronald M; Carraro, Nicola

    2014-01-01

    The PIN and ACO gene families present interesting questions about the evolution of plant physiology, including testing hypotheses about the ecological drivers of their diversification and whether unrelated genes have been recruited for similar functions. The PIN-formed proteins contribute to the polar transport of auxin, a hormone which regulates plant growth and development. PIN loci are categorized into groups according to their protein length and structure, as well as subcellular localization. An interesting question with PIN genes is the nature of the ancestral form and location. ACOs are members of a superfamily of oxygenases and oxidases that catalyze the last step of ethylene synthesis, which regulates many aspects of the plant life cycle. We used publicly available PIN and ACO sequences to conduct phylogenetic analyses. Third codon positions of these genes in monocots have a high GC content, which could be historical but is more likely due to a mutational bias. Thus, we developed methods to extract phylogenetic information from nucleotide sequences while avoiding this convergent feature. One method consisted in using only A-T transformations, and another used only the first and second codon positions for serine, which can only take A or T and G or C, respectively. We also conducted tree-searches for both gene families using unaligned amino acid sequences and dynamic homology. PIN genes appear to have diversified earlier than ACOs, with monocot and dicot copies more mixed in the phylogeny. However, gymnosperm PINs appear to be derived and not closely related to those from primitive plants. We find strong support for a long PIN gene ancestor with short forms subsequently evolving one or more times. ACO genes appear to have diversified mostly since the dicot-monocot split, as most genes cluster into a small number of monocot and dicot clades when the tree is rooted by genes from mosses. Gymnosperm ACOs were recovered as closely related and derived.

  14. Compositional mining of multiple object API protocols through state abstraction.

    Science.gov (United States)

    Dai, Ziying; Mao, Xiaoguang; Lei, Yan; Qi, Yuhua; Wang, Rui; Gu, Bin

    2013-01-01

    API protocols specify correct sequences of method invocations. Despite their usefulness, API protocols are often unavailable in practice because writing them is cumbersome and error prone. Multiple object API protocols are more expressive than single object API protocols. However, the huge number of objects of typical object-oriented programs poses a major challenge to the automatic mining of multiple object API protocols: besides maintaining scalability, it is important to capture various object interactions. Current approaches utilize various heuristics to focus on small sets of methods. In this paper, we present a general, scalable, multiple object API protocols mining approach that can capture all object interactions. Our approach uses abstract field values to label object states during the mining process. We first mine single object typestates as finite state automata whose transitions are annotated with states of interacting objects before and after the execution of the corresponding method and then construct multiple object API protocols by composing these annotated single object typestates. We implement our approach for Java and evaluate it through a series of experiments.

  15. The pheromones of laying workers in two honeybee sister species: Apis cerana and Apis mellifera.

    Science.gov (United States)

    Tan, Ken; Yang, Mingxian; Wang, Zhengwei; Radloff, Sarah E; Pirk, Christian W W

    2012-04-01

    When a honeybee colony loses its queen, workers activate their ovaries and begin to lay eggs. This is accompanied by a shift in their pheromonal bouquet, which becomes more queen like. Workers of the Asian hive bee Apis cerana show unusually high levels of ovary activation and this can be interpreted as evidence for a recent evolutionary arms race between queens and workers over worker reproduction in this species. To further explore this, we compared the rate of pheromonal bouquet change between two honeybee sister species of Apis cerana and Apis mellifera under queenright and queenless conditions. We show that in both species, the pheromonal components HOB, 9-ODA, HVA, 9-HDA, 10-HDAA and 10-HDA have significantly higher amounts in laying workers than in non-laying workers. In the queenright colonies of A. mellifera and A. cerana, the ratios (9-ODA)/(9-ODA + 9-HDA + 10-HDAA + 10-HDA) are not significantly different between the two species, but in queenless A. cerana colonies the ratio is significant higher than in A. mellifera, suggesting that in A. cerana, the workers' pheromonal bouquet is dominated by the queen compound, 9-ODA. The amount of 9-ODA in laying A. cerana workers increased by over 585% compared with the non-laying workers, that is 6.75 times higher than in A. mellifera where laying workers only had 86% more 9-ODA compared with non-laying workers.

  16. A framework for API solubility modelling

    DEFF Research Database (Denmark)

    Conte, Elisa; Gani, Rafiqul; Crafts, Peter

    The solubility of solid organic compounds in water and organic solvents is a fundamental thermodynamic property for many purposes such as product-process design and optimization, for the chemical and pharmaceutical industry. Experimental literature solubility data are usually scarce and temperature......-dependent measurements are expensive in terms of time and resources. The few available data are badly organized and difficult to use for fast solubility calculations and solvent screening. Available models often require time consuming and complex implementation together with a good user expertise for their efficient use....... In addition, most of the models are not predictive and requires experimental data for the calculation of the needed parameters. This work aims at developing an efficient framework for the solubility modelling of Active Pharmaceutical Ingredients (API) in water and organic solvents. With this framework...

  17. Diagnóstico cardíaco a partir de dados acústicos e clínicos

    OpenAIRE

    Cruz, Elisete Maria Silva Dias Alves da

    2015-01-01

    Este documento foi redigido no âmbito da dissertação do Mestrado em Engenharia Informática na área de Arquiteturas, Sistemas e Redes, do Departamento de Engenharia Informática, do ISEP, cujo tema é diagnóstico cardíaco a partir de dados acústicos e clínicos. O objetivo deste trabalho é produzir um método que permita diagnosticar automaticamente patologias cardíacas utilizando técnicas de classificação de data mining. Foram utilizados dois tipos de dados: sons cardíacos gravados...

  18. Studies on Pumice Lightweight Aggregate Concrete with Quarry Dust Using Mathematical Modeling Aid of ACO Techniques

    Directory of Open Access Journals (Sweden)

    J. Rex

    2016-01-01

    Full Text Available The lightweight aggregate is an aggregate that weighs less than the usual rock aggregate and the quarry dust is a rock particle used in the concrete for the experimentation. The significant intention of the proposed technique is to frame a mathematical modeling with the aid of the optimization techniques. The mathematical modeling is done by minimizing the cost and time consumed in the case of extension of the real time experiment. The proposed mathematical modeling is utilized to predict four output parameters such as compressive strength (Mpa, split tensile strength (Mpa, flexural strength (Mpa, and deflection (in mm. Here, the modeling is carried out with three different optimization techniques like genetic algorithm (GA, particle swarm optimization (PSO, and ant colony optimization (ACO with 80% of data from experiment utilized for the training and the remaining 20% for the validation. Finally, while testing, the error value is minimized and the performance obtained in the ACO for the parameters such as compressive strength, split tensile strength, flexural strength, and deflection is 91%, 98%, 87%, and 94% of predicted values, respectively, in the mathematical modeling.

  19. Optimization of Vibration Reduction Ability of Ladder Tracks by FEM Coupled with ACO

    Directory of Open Access Journals (Sweden)

    Hao Jin

    2015-01-01

    Full Text Available Ladder track, which has drawn increased attention in scientific communities, is an effective method for reducing vibrations from underground railways. In order to optimize the vibration reduction ability of ladder track, a new method, that is, the finite element method (FEM coupled with ant colony optimization (ACO, has been proposed in this paper. We describe how to build the FEM model verified by the vibration tests in the Track Vibration Abatement and Control Laboratory and how to couple the FEM with ACO. The density and elasticity modulus of the sleeper pad are optimized using this method. After optimization, the vibration acceleration level of the supporting platform in the 1–200 Hz range was reduced from 102.8 dB to 94.4 dB. The optimized density of the sleeper pad is 620 kg/m3, and the optimized elasticity modulus of the sleeper pad is 6.25 × 106 N/m2.

  20. Application of GA, PSO, and ACO Algorithms to Path Planning of Autonomous Underwater Vehicles

    Institute of Scientific and Technical Information of China (English)

    Mohammad Pourmahmood Aghababa; Mohammad Hossein Amrollahi; Mehdi Borjkhani

    2012-01-01

    In this paper,an underwater vehicle was modeled with six dimensional nonlinear equations of motion,controlled by DC motors in all degrees of freedom.Near-optimal trajectories in an energetic environment for underwater vehicles were computed using a numerical solution of a nonlinear optimal control problem (NOCP).An energy performance index as a cost function,which should be minimized,was defined.The resulting problem was a two-point boundary value problem (TPBVP).A genetic algorithm (GA),particle swarm optimization (PSO),and ant colony optimization (ACO) algorithms were applied to solve the resulting TPBVP.Applying an Euler-Lagrange equation to the NOCE a conjugate gradient penalty method was also adopted to solve the TPBVP.The problem of energetic environments,involving some energy sources,was discussed.Some near-optimal paths were found using a GA,PSO,and ACO algorithms.Finally,the problem of collision avoidance in an energetic environment was also taken into account.

  1. Building Hypermedia APIs with HTML5 and Node

    CERN Document Server

    Amundsen, Mike

    2011-01-01

    With this concise book, you'll learn the art of building hypermedia APIs that don't simply run on the Web, but that actually exist in the Web. You'll start with the general principles and technologies behind this architectural approach, and then dive hands-on into three fully-functional API examples. Too many APIs rely on concepts rooted in desktop and local area network patterns that don't scale well-costly solutions that are difficult to maintain over time. This book shows system architects and web developers how to design and implement human- and machine-readable web services that remain

  2. Practical Implementation of 10 Rules for Writing REST APIs

    Directory of Open Access Journals (Sweden)

    Jiri Hradil

    2017-01-01

    Full Text Available This paper shows a practical implementation of “10 Rules for Writing REST APIs introduced in the article” (Hradil, 2016. The application is done in Invoice Home (Wikilane, 2016, an invoicing web application for small business and entrepreneurs available world-wide. The API is implemented in JSON hypermedia format (ECMA International, 2016 and with Ruby on Rails framework (Hansson, 2016. The main purpose of the API is to allow connection of Invoice Home with external systems and offer Invoice Home data in simpler format compared to the current HTML format of the full-stack web application. The paper could be also used as a basic template or pattern for any other implementation of the JSON API in any web-based application.

  3. Drone competition at drone congregation areas in four Apis species

    OpenAIRE

    2005-01-01

    International audience; In Apis mellifera the estimated average number of drones visiting a drone congregation area (DCA) was 11 750 + 2 145. Drones of the species Apis cerana, A. koschevnikovi, A. dorsata and A. mellifera, which pursued a queen dummy moving in circular course, flew in a comet shaped formation. Median numbers of drones in a comet ranged from 9 drones (A. koschevnikovi) to 31 drones (A. mellifera). In none of the species we observed aggression between drones. Drone density beh...

  4. Pengaruh Busur Api Terhadap Kekuatan Dielektrik Gas SF6

    OpenAIRE

    Jhony

    2011-01-01

    Bahan isolasi gas yang sering digunakan adalah gas SF6. Sifat –sifat dari gas SF6 ini tidak berwarna, tidak beracun, tidak berbau. Salah satu pemanfaatan dari gas SF6 ini sebagai bahan isolasi adalah pada pemutus tenaga (circuit breaker). Jika pemutus tenaga SF6 membuka, maka pada sela kontaknya akan terjadi busur api. Busur api akan menaikkan temperatur gas SF6 sehingga ada dugaan gas SF6 mengalami perubahan struktur kimia, sehingga sifat listriknya berubah. Tugas akhir ini ak...

  5. NORMATIVE VALUES OF EQ-5D-5L FOR DIABETES PATIENTS FROM SPAIN.

    Science.gov (United States)

    Collado Mateo, Daniel; García Gordillo, Miguel A; Olivares, Pedro R; Adsuar, José C

    2015-10-01

    Introducción: la diabetes es una enfermedad metabólica que puede conllevar una reducción de la calidad de vida relacionada con la salud. El EQ-5D es un cuestionario genérico de calidad de vida relacionada con la salud basado en preferencias sociales. Este cuestionario ha sido muy utilizado en pacientes con diabetes. Objetivo: el objetivo del presente artículo es informar sobre los valores normativos del cuestionario EQ-5D-5L en personas españolas con diabetes. Métodos: se utilizaron datos de la Encuesta Española de Salud (2011/2012). Un total de 1.857 personas con diabetes participaron en la encuesta. La puntuación del EQ-5D-5L se ha reflejado en función del sexo, región (incluyendo las 17 comunidades autónomas y las 2 ciudades autónomas de España), y 8 grupos de edad. Resultados: la media del índice de utilidad para toda la muestra fue de 0,742. Esta fue mejor para hombres (0,826) que para mujeres (0,673). Resultados similares se observaron en la Escala Visual Analógica. El efecto techo fue mucho mayor en hombres (44,83%) que en mujeres (24,41%). Conclusiones: el presente estudio recoge datos normativos representativos del EQ-5D-5L en España de personas con diabetes.

  6. NHETS - Estudo de Necrópsias de Pacientes Submetidos a Transplante Cardíaco

    Directory of Open Access Journals (Sweden)

    Thiago Ninck Valette

    2014-06-01

    Full Text Available Fundamento: Discordâncias entre diagnóstico pre e post-mortem são relatadas na literatura, podendo variar de 4,1 a 49,8% dentre os casos encaminhados para exame necroscópico, com importante repercussão no tratamento dos pacientes. Objetivo: Analisar pacientes com óbito após o transplante cardíaco e confrontar os diagnósticos pre e post-mortem. Métodos: Por meio da revisão de prontuários, foram analisados dados clínicos, presença de comorbidades, esquema de imunossupressão, exames laboratoriais, causa clínica do óbito e causa do óbito à necrópsia. Foram confrontadas, então, a causa clínica e a causa necroscópica do óbito de cada paciente. Resultados: Foram analisados 48 óbitos submetidos à necrópsia no período de 2000 a 2010; 29 (60,4% tiveram diagnósticos clínico e necroscópico concordantes, 16 (33,3% tiveram diagnósticos discordantes e três (6,3% tiveram diagnóstico não esclarecido. Entre os discordantes, 15 (31,3% apresentaram possível impacto na sobrevida e um (2,1% não apresentou impacto na sobrevida. O principal diagnóstico clínico feito equivocadamente foi o de infecção, com cinco casos (26,7% dos discordantes, seguido por rejeição hiperaguda, com quatro casos (20% dos discordantes, e tromboembolismo pulmonar, com três casos (13,3% dos discordantes. Conclusão: Discordâncias entre o diagnóstico clínico e achados da necrópsia são comumente encontradas no transplante cardíaco. Novas estratégias no aperfeiçoamento do diagnóstico clínico devem ser introduzidas, considerando-se os resultados da necrópsia para melhoria do tratamento da insuficiência cardíaca por meio do transplante cardíaco.

  7. Quantifying the effects of different post-processing quality filters on regionally and temporally averaged ACOS XCO2

    Science.gov (United States)

    Fisher, B.; O'Dell, C.; Mandrake, L.

    2013-12-01

    The Atmospheric CO2 Observations from Space (ACOS) group has been producing and distributing total column CO2 (XCO2) products using JAXA/NIES/MOE Greenhouse Gases Observing SATellite (GOSAT) spectra and has accumulated almost 4 years of data with version 3.3. While the ACOS team strives to only process soundings that the retrieval algorithm can handle well, we are conservative in what we reject from processing. Consequently, some soundings get processed which do not yield reliable results. We have developed post-processing filters based on comparisons to a few truth proxies (model means, TCCON, and the southern hemisphere approximation) to flag the less reliable soundings. Here we compare regionally (using TRANSCOM spatial bins) and monthly averaged XCO2 that have been filtered by our normal method (described in the ACOS Level 2 Data User's Guide) and a newer method, which we have named warn levels. Mean XCO2 differences are quantified spatially and temporally to inform possible biases in carbon cycle studies that could potentially be introduced by the application of differing post-processing screening methodologies to the ACOS products.

  8. GIANT API: an application programming interface for functional genomics

    Science.gov (United States)

    Roberts, Andrew M.; Wong, Aaron K.; Fisk, Ian; Troyanskaya, Olga G.

    2016-01-01

    GIANT API provides biomedical researchers programmatic access to tissue-specific and global networks in humans and model organisms, and associated tools, which includes functional re-prioritization of existing genome-wide association study (GWAS) data. Using tissue-specific interaction networks, researchers are able to predict relationships between genes specific to a tissue or cell lineage, identify the changing roles of genes across tissues and uncover disease-gene associations. Additionally, GIANT API enables computational tools like NetWAS, which leverages tissue-specific networks for re-prioritization of GWAS results. The web services covered by the API include 144 tissue-specific functional gene networks in human, global functional networks for human and six common model organisms and the NetWAS method. GIANT API conforms to the REST architecture, which makes it stateless, cacheable and highly scalable. It can be used by a diverse range of clients including web browsers, command terminals, programming languages and standalone apps for data analysis and visualization. The API is freely available for use at http://giant-api.princeton.edu. PMID:27098035

  9. Mining Effective Temporal Specifications from Heterogeneous API Data

    Institute of Scientific and Technical Information of China (English)

    Qian Wu; Guang-Tai Liang; Qian-Xiang Wang; Hong Mei

    2011-01-01

    Temporal specifications for Application Programming Interfaces (APIs) serve as an important basis for many defect detection tools.As these specifications are often not well documented,various approaches have been proposed to automatically mine specifications typically from API library source code or from API client programs.However,the library-based approaches take substantial computational resources and produce rather limited useful specifications,while the client-based approaches suffer from high false positive rates.To address the issues of existing approaches,we propose a novel specification miniug approach,called MineHEAD,which exploits heterogeneous API data,including information from API client programs as well as API library source code and comments,to produce effective specifications for defect detection with low cost.In particular,MineHEAD first applies client-based specification mining to produce a collection of candidate specifications,and then exploits the related library source code and comments to identify and refine the real specifications from the candidates.Our evaluation results on nine open source projects show that MineHEAD produces effective specifications with average precision of 97.2%.

  10. GIANT API: an application programming interface for functional genomics.

    Science.gov (United States)

    Roberts, Andrew M; Wong, Aaron K; Fisk, Ian; Troyanskaya, Olga G

    2016-07-08

    GIANT API provides biomedical researchers programmatic access to tissue-specific and global networks in humans and model organisms, and associated tools, which includes functional re-prioritization of existing genome-wide association study (GWAS) data. Using tissue-specific interaction networks, researchers are able to predict relationships between genes specific to a tissue or cell lineage, identify the changing roles of genes across tissues and uncover disease-gene associations. Additionally, GIANT API enables computational tools like NetWAS, which leverages tissue-specific networks for re-prioritization of GWAS results. The web services covered by the API include 144 tissue-specific functional gene networks in human, global functional networks for human and six common model organisms and the NetWAS method. GIANT API conforms to the REST architecture, which makes it stateless, cacheable and highly scalable. It can be used by a diverse range of clients including web browsers, command terminals, programming languages and standalone apps for data analysis and visualization. The API is freely available for use at http://giant-api.princeton.edu.

  11. Identification of Complete Repertoire of Apis florea Odorant Receptors Reveals Complex Orthologous Relationships with Apis mellifera.

    Science.gov (United States)

    Karpe, Snehal D; Jain, Rikesh; Brockmann, Axel; Sowdhamini, Ramanathan

    2016-09-26

    We developed a computational pipeline for homology based identification of the complete repertoire of olfactory receptor (OR) genes in the Asian honey bee species, Apis florea Apis florea is phylogenetically the most basal honey bee species and also the most distant sister species to the Western honey bee Apis mellifera, for which all OR genes had been identified before. Using our pipeline, we identified 180 OR genes in A. florea, which is very similar to the number of ORs identified in A. mellifera (177 ORs). Many characteristics of the ORs including gene structure, synteny of tandemly repeated ORs and basic phylogenetic clustering are highly conserved. The composite phylogenetic tree of A. florea and A. mellifera ORs could be divided into 21 clades which are in harmony with the existing Hymenopteran tree. However, we found a few nonorthologous OR relationships between both species as well as independent pseudogenization of ORs suggesting separate evolutionary changes. Particularly, a subgroup of the OR gene clade XI, which had been hypothesized to code cuticular hydrocarbon receptors showed a high number of species-specific ORs RNAseq analysis detected a total number of 145 OR transcripts in male and 162 in female antennae. Most of the OR genes were highly expressed on the female antennae. However, we detected five distinct male-biased OR genes, out of which three genes (AfOr11, AfOr18, AfOr170P) were shown to be male-biased in A. mellifera, too, thus corroborating a behavioral function in sex-pheromone communication.

  12. Variance in spermatozoa number among Apis dorsata drones and among Apis mellifera drones

    OpenAIRE

    Koeniger, Gudrun; KOENIGER, Nikolaus; Tingek, Salim; Phiancharoen, Mananya

    2005-01-01

    International audience; Published estimates of the mean spermatozoa numbers for Apis dorsata drones vary from 1.2 × 106 and 2.4 × 106; the number of spermatozoa per individual drone vary from 0.22 × 106 to 2.65 × 106. Counts presented here revealed 1.19 × 106 + 0.25 × 106 spermatozoa in drones sampled near a colony and 1.59 × 106 + 0.18 × 106 in drones sampled at a drone congregation area (DCA) in Sabah, Borneo. The difference between the two sites is significant. Further, the degree of varia...

  13. Update Strength in EDAs and ACO: How to Avoid Genetic Drift

    DEFF Research Database (Denmark)

    Sudholt, Dirk; Witt, Carsten

    2016-01-01

    , showing that the update strength should be limited to 1/K, ρ = O(1/(√n log n)). In fact, choosing 1/K, ρ ∼ 1/(√n log n) both algorithms efficiently optimize OneMax in expected time O (n log n). Our analyses provide new insights into the stochastic behavior of probabilistic model-building GAs and propose......We provide a rigorous runtime analysis concerning the update strength, a vital parameter in probabilistic model-building GAs such as the step size 1/K in the compact Genetic Algorithm (cGA) and the evaporation factor ρ in ACO. While a large update strength is desirable for exploitation...

  14. Avaliação do sopro cardíaco na infância

    OpenAIRE

    Kobinger,Maria Elisabeth B.A.

    2003-01-01

    OBJETIVO: discutir a avaliação clínica e laboratorial do sopro cardíaco, considerando sua alta freqüência no atendimento ambulatorial do pediatra geral. FONTE DE DADOS: revisão baseada na análise crítica da literatura atual e consulta a compêndios de cardiologia pediátrica e pediatria contendo informações básicas sobre o tema. SÍNTESE DOS DADOS: os principais destaques do artigo referem-se à importância da anamnese e exame físico do sistema cardiovascular, essenciais para o pediatra geral dia...

  15. Rabdomiomas Intracardíacos em Recém-nascido: ...Pensou em Esclerose Tuberosa?

    OpenAIRE

    Costa, Andreia; Baptista, Maria João; Viera, António; Temudo, Teresa

    2014-01-01

    Os autores descrevem o caso clínico de um recém-nascido com rabdomiomas cardíacos que se manifestaram pela detecção à nascença de frustes alterações auscultatórias cardíacas. O diagnóstico definitivo de Esclerose Tuberosa foi feito no período neonatal, com posterior expressão multissístémica da doença. Os autores fazem uma breve revisão teórica desta entidade clínica, no que concerne à sua eliopatogénese, critérios diagnósticos, espectro clínico, laboratorial e imagiológico, e abordagem terap...

  16. Honeybee (Apis mellifera ligustica) drone embryo proteomes.

    Science.gov (United States)

    Li, Jianke; Fang, Yu; Zhang, Lan; Begna, Desalegn

    2011-03-01

    Little attention has been paid to the drone honeybee (Apis mellifera ligustica) which is a haploid individual carrying only the set of alleles that it inherits from its mother. Molecular mechanisms underlying drone embryogenesis are poorly understood. This study evaluated protein expression profiles of drone embryogenesis at embryonic ages of 24, 48 and 72h. More than 100 reproducible proteins were analyzed by mass spectrometry on 2D electrophoresis gels. Sixty-two proteins were significantly changed at the selected three experimental age points. Expression of the metabolic energy requirement-related protein peaked at the embryonic age of 48h, whereas development and metabolizing amino acid-related proteins expressed optimally at 72h. Cytoskeleton, protein folding and antioxidant-related proteins were highly expressed at 48 and 72h. Protein networks of the identified proteins were constructed and protein expressions were validated at the transcription level. This first proteomic study of drone embryogenesis in the honeybee may provide geneticists an exact timetable and candidate protein outline for further manipulations of drone stem cells.

  17. The Apis mellifera Filamentous Virus Genome

    Directory of Open Access Journals (Sweden)

    Laurent Gauthier

    2015-07-01

    Full Text Available A complete reference genome of the Apis mellifera Filamentous virus (AmFV was determined using Illumina Hiseq sequencing. The AmFV genome is a double stranded DNA molecule of approximately 498,500 nucleotides with a GC content of 50.8%. It encompasses 247 non-overlapping open reading frames (ORFs, equally distributed on both strands, which cover 65% of the genome. While most of the ORFs lacked threshold sequence alignments to reference protein databases, twenty-eight were found to display significant homologies with proteins present in other large double stranded DNA viruses. Remarkably, 13 ORFs had strong similarity with typical baculovirus domains such as PIFs (per os infectivity factor genes: pif-1, pif-2, pif-3 and p74 and BRO (Baculovirus Repeated Open Reading Frame. The putative AmFV DNA polymerase is of type B, but is only distantly related to those of the baculoviruses. The ORFs encoding proteins involved in nucleotide metabolism had the highest percent identity to viral proteins in GenBank. Other notable features include the presence of several collagen-like, chitin-binding, kinesin and pacifastin domains. Due to the large size of the AmFV genome and the inconsistent affiliation with other large double stranded DNA virus families infecting invertebrates, AmFV may belong to a new virus family.

  18. The Apis mellifera Filamentous Virus Genome.

    Science.gov (United States)

    Gauthier, Laurent; Cornman, Scott; Hartmann, Ulrike; Cousserans, François; Evans, Jay D; de Miranda, Joachim R; Neumann, Peter

    2015-07-09

    A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double stranded DNA molecule of approximately 498,500 nucleotides with a GC content of 50.8%. It encompasses 247 non-overlapping open reading frames (ORFs), equally distributed on both strands, which cover 65% of the genome. While most of the ORFs lacked threshold sequence alignments to reference protein databases, twenty-eight were found to display significant homologies with proteins present in other large double stranded DNA viruses. Remarkably, 13 ORFs had strong similarity with typical baculovirus domains such as PIFs (per os infectivity factor genes: pif-1, pif-2, pif-3 and p74) and BRO (Baculovirus Repeated Open Reading Frame). The putative AmFV DNA polymerase is of type B, but is only distantly related to those of the baculoviruses. The ORFs encoding proteins involved in nucleotide metabolism had the highest percent identity to viral proteins in GenBank. Other notable features include the presence of several collagen-like, chitin-binding, kinesin and pacifastin domains. Due to the large size of the AmFV genome and the inconsistent affiliation with other large double stranded DNA virus families infecting invertebrates, AmFV may belong to a new virus family.

  19. The human CD5L/AIM-CD36 axis: A novel autophagy inducer in macrophages that modulates inflammatory responses.

    Science.gov (United States)

    Sanjurjo, Lucía; Amézaga, Núria; Aran, Gemma; Naranjo-Gómez, Mar; Arias, Lilibeth; Armengol, Carolina; Borràs, Francesc E; Sarrias, Maria-Rosa

    2015-01-01

    CD5L (CD5 molecule-like) is a secreted glycoprotein that participates in host response to bacterial infection. CD5L influences the monocyte inflammatory response to the bacterial surface molecules lipopolysaccharide (LPS) and lipoteichoic acid (LTA) by inhibiting TNF secretion. Here we studied the intracellular events that lead to macrophage TNF inhibition by human CD5L. To accomplish this goal, we performed functional analyses with human monocytic THP1 macrophages, as well as with peripheral blood monocytes. Inhibition of phosphatidylinositol 3-kinase (PtdIns3K) reversed the inhibitory effect of CD5L on TNF secretion. Among the various PtdIns3K isoforms, our results indicated that CD5L activates PtdIns3K (whose catalytic subunit is termed PIK3C3), a key modulator involved in autophagy. Further analysis revealed a concomitant enhancement of autophagy markers such as cellular LC3-II content, increased LC3 puncta, as well as LC3-LysoTracker Red colocalization. Moreover, electron microscopy showed an increased presence of cytosolic autophagosomes in THP1 macrophages overexpressing CD5L. Besides preventing TNF secretion, CD5L also inhibited IL1B and enhanced IL10 secretion. This macrophage anti-inflammatory pattern of CD5L was reverted upon silencing of autophagy protein ATG7 by siRNA transfection. Additional siRNA experiments in THP1 macrophages indicated that the induction of autophagy mechanisms by CD5L was achieved through cell-surface scavenger receptor CD36, a multiligand receptor expressed in a wide variety of cell types. Our data represent the first evidence that CD36 is involved in autophagy and point to a significant contribution of the CD5L-CD36 axis to the induction of macrophage autophagy.

  20. Inhibiting DNA methylation alters olfactory extinction but not acquisition learning in Apis cerana and Apis mellifera.

    Science.gov (United States)

    Gong, Zhiwen; Wang, Chao; Nieh, James C; Tan, Ken

    2016-07-01

    DNA methylation plays a key role in invertebrate acquisition and extinction memory. Honey bees have excellent olfactory learning, but the role of DNA methylation in memory formation has, to date, only been studied in Apis mellifera. We inhibited DNA methylation by inhibiting DNA methyltransferase (DNMT) with zebularine (zeb) and studied the resulting effects upon olfactory acquisition and extinction memory in two honey bee species, Apis cerana and A. mellifera. We used the proboscis extension reflex (PER) assay to measure memory. We provide the first demonstration that DNA methylation is also important in the olfactory extinction learning of A. cerana. DNMT did not reduce acquisition learning in either species. However, zeb bidirectionally and differentially altered extinction learning in both species. In particular, zeb provided 1h before acquisition learning improved extinction memory retention in A. mellifera, but reduced extinction memory retention in A. cerana. The reasons for these differences are unclear, but provide a basis for future studies to explore species-specific differences in the effects of methylation on memory formation.

  1. Certeza diagnóstica en la mortalidad de una población de pacientes con trasplante cardíaco

    OpenAIRE

    2006-01-01

    RESUMENIntroducciónA pesar de que la morbimortalidad en el trasplante cardíaco ha sido motivo de extensoanálisis, la mayoría de los estudios y registros de mortalidad en pacientes trasplantados sebasan sobre datos clínicos. En la bibliografía existen comunicaciones aisladas de autopsiasen pacientes con trasplante cardíaco.ObjetivoDeterminar la importancia de la realización de estudios anatomopatológicos para el diagnósticode causa de muerte en un programa de trasplante cardíaco.Material y mét...

  2. A quick guide to API 510 certified pressure vessel inspector syllabus example questions and worked answers

    CERN Document Server

    Matthews, Clifford

    2010-01-01

    The API Individual Certification Programs (ICPs) are well established worldwide in the oil, gas, and petroleum industries. This Quick Guide is unique in providing simple, accessible and well-structured guidance for anyone studying the API 510 Certified Pressure Vessel Inspector syllabus by summarizing and helping them through the syllabus and providing multiple example questions and worked answers.Technical standards are referenced from the API 'body of knowledge' for the examination, i.e. API 510 Pressure vessel inspection, alteration, rerating; API 572 Pressure vessel inspection; API

  3. Application of Celluclast 1.5L in apple pectin extraction.

    Science.gov (United States)

    Wikiera, Agnieszka; Mika, Magdalena; Starzyńska-Janiszewska, Anna; Stodolak, Bożena

    2015-12-10

    Pectins were extracted from apple pomace with Celluclast 1.5L at a dose of 25, 50 and 75 μl per 1g of material. In obtained pectin, the galacturonic acid (GalA) content, the neutral sugars (NS) profile, the degree of methylation (DM) and acetylation (DAc), the molecular mass, protein, ash and polyphenol levels as well as antioxidant and antitumor activity were determined. The lowest dose of enzymatic preparation resulted in the yield of pectin isolation comparable with acidic treatment (15.3%). Application of higher dose caused further, almost 4% increase in polymer recovery. Enzymatically isolated pectin was characterised by larger molecular mass and contained more GalA of higher DM and DAc than polymer extracted with acid. It was also richer in protein and polyphenols, and had different NS profile, which resulted in higher antiradical activity as well as the ability to inhibit the proliferation and invasion of Caco-2 adenocarcinoma cells.

  4. 3.5L V6 EcoBoost - democratization of sustainable engine technology

    Energy Technology Data Exchange (ETDEWEB)

    Kapp, Daniel; Schamel, Andreas; Hinds, Brett; Weaver, Corey [Ford Motor Co., Dearborn, MI (United States)

    2008-07-01

    The North American automotive industry is presently facing the challenge of a recently signed energy bill increasing corporate average fuel economy standards to 35 mpg (6.7 L/km) by 2020, inclusive of cars and trucks. The passing of this bill is coincident with crude oil costs hovering in the $130USD / barrel range, retail gasoline costs in the $4USD / gallon range, and mounting evidence on CO2 emissions and global warming. Ford Motor Company has anticipated these trends, has invested in the development of fuel efficient engine technologies, and is now deploying these technologies in volume to deliver both a meaningful and sustainable response to the above challenges. Leading the deployment curve is a technology publically announced as EcoBoost, representing the application of direct fuel injection and turbocharging to an advanced gasoline engine. Ford has previously reported the fundamentals of this technology which enables a significant fuel economy improvement via engine downsizing, and a significant performance improvement via engine dynamic response. As applied to 14 and V6 engine architectures, Ford has announced plans to deploy up to 750,000 EcoBoost engines worldwide by 2013 CY, with first application in the form of a 3.5L V6 EcoBoost engine in the 2010 MY Lincoln MKS luxury sedan. This paper summaries the development of the 3.5L V6 EcoBoost engine and its associated as-installed systems. Combustion, fuel, boost, and power conversion systems are discussed in detail with emphasis on the efficiency and value of these systems which enable a singular engine design to be applied across a wide range of applications. Engine performance and fuel economy assessments are also discussed and compared to conventional gasoline engines. (orig.)

  5. A knowledge discovery object model API for Java

    Directory of Open Access Journals (Sweden)

    Jones Steven JM

    2003-10-01

    Full Text Available Abstract Background Biological data resources have become heterogeneous and derive from multiple sources. This introduces challenges in the management and utilization of this data in software development. Although efforts are underway to create a standard format for the transmission and storage of biological data, this objective has yet to be fully realized. Results This work describes an application programming interface (API that provides a framework for developing an effective biological knowledge ontology for Java-based software projects. The API provides a robust framework for the data acquisition and management needs of an ontology implementation. In addition, the API contains classes to assist in creating GUIs to represent this data visually. Conclusions The Knowledge Discovery Object Model (KDOM API is particularly useful for medium to large applications, or for a number of smaller software projects with common characteristics or objectives. KDOM can be coupled effectively with other biologically relevant APIs and classes. Source code, libraries, documentation and examples are available at http://www.bcgsc.ca/bioinfo/software.

  6. Thermodynamic phase behavior of API/polymer solid dispersions.

    Science.gov (United States)

    Prudic, Anke; Ji, Yuanhui; Sadowski, Gabriele

    2014-07-07

    To improve the bioavailability of poorly soluble active pharmaceutical ingredients (APIs), these materials are often integrated into a polymer matrix that acts as a carrier. The resulting mixture is called a solid dispersion. In this work, the phase behaviors of solid dispersions were investigated as a function of the API as well as of the type and molecular weight of the carrier polymer. Specifically, the solubility of artemisinin and indomethacin was measured in different poly(ethylene glycol)s (PEG 400, PEG 6000, and PEG 35000). The measured solubility data and the solubility of sulfonamides in poly(vinylpyrrolidone) (PVP) K10 and PEG 35000 were modeled using the perturbed-chain statistical associating fluid theory (PC-SAFT). The results show that PC-SAFT predictions are in a good accordance with the experimental data, and PC-SAFT can be used to predict the whole phase diagram of an API/polymer solid dispersion as a function of the kind of API and polymer and of the polymer's molecular weight. This remarkably simplifies the screening process for suitable API/polymer combinations.

  7. The Marker Gene ACO of Fruits Preservation%果树果实保鲜力强弱标记基因ACO

    Institute of Scientific and Technical Information of China (English)

    张太奎; 纵丹; 朱芳明; 贺笑; 刘惠民; 张汉尧

    2015-01-01

    果树果实的成熟期与保鲜是影响其经济价值的重要因素,ACC氧化酶是植物乙烯合成途径中的重要酶,催化ACC合成乙烯,进而催化果实的成熟.研究ACC氧化酶基因(ACO)对标记和提高果实的保鲜力具有重要意义.本研究对中华猕猴桃(Actinidia chinensis)、美味猕猴桃(Actinidia deliciosa)、杏(Prunus armeniaca)、葡萄(Vitis vinifera)、苹果(Malus domestica)、西洋梨(Pyrus communis)、沙梨(Pyrus pyrifolia)、桃(Prunus persica)、梅(Prunus mume)、大蕉(Musa ABB)10种果树的ACO基因进行氨基酸序列、蛋白质理化性质及结构、系统发育等生物信息学进行分析,并对这10种果树进行聚类.结果表明:(1)苹果较梅更耐储藏,桃与杏保鲜能力相当,西洋梨和沙梨保鲜力相当,中华猕猴桃和美味猕猴桃保鲜力相当;(2) ACO基因可用于同科物种间保鲜能力的选择,这可能与ACO基因编码氨基酸序列18位点处变异、2OG-Fell Oxy基因家族的高度保守和ACO的多元作用机制有关;(3) ACO的作用机制为在游离核糖体上合成稳定的亲水性蛋白质后,不经蛋白转运,直接在细胞膜内表面氧化ACC合成乙烯,受基因型和外界刺激的多重影响.因此,果树ACO基因可用于属间物种间果实保鲜能力的选择,是一个重要的功能基因.

  8. Nosema ceranae in drone honey bees (Apis mellifera).

    Science.gov (United States)

    Traver, Brenna E; Fell, Richard D

    2011-07-01

    Nosema ceranae is a microsporidian intracellular parasite of honey bees, Apis mellifera. Previously Nosema apis was thought to be the only cause of nosemosis, but it has recently been proposed that N. ceranae is displacing N. apis. The rapid spread of N. ceranae could be due to additional transmission mechanisms, as well as higher infectivity. We analyzed drones for N. ceranae infections using duplex qPCR with species specific primers and probes. We found that both immature and mature drones are infected with N. ceranae at low levels. This is the first report detecting N. ceranae in immature bees. Our data suggest that because drones are known to drift from their parent hives to other hives, they could provide a means for disease spread within and between apiaries.

  9. Acinetobacter apis sp. nov., isolated from the intestinal tract of a honey bee, Apis mellifera.

    Science.gov (United States)

    Kim, Pil Soo; Shin, Na-Ri; Kim, Joon Yong; Yun, Ji-Hyun; Hyun, Dong-Wook; Bae, Jin-Woo

    2014-08-01

    A novel Gram-negative, obligate aerobic, non-motile, and both coccobacillus- and bacillus-shaped bacterium, designated strain HYN18(T), was isolated from the intestinal tract of a honey bee (Apis mellifera). The isolate was oxidase-negative and catalase-positive. Strain HYN18(T) showed optimum growth at 25°C, pH 6-7, and in the presence of 1% (w/v) NaCl in trypticase soy broth medium. The isolate was negative for hydrolyses of starch, casein, gelatin and urea, indole production from tryptone and hemolysis on sheep blood agar. A phylogenetic analysis based on the 16S rRNA gene and rpoB gene sequence showed that strain HYN18(T) was most closely related to Acinetobacter nectaris SAP 763.2(T) and A. boissieri SAP 284.1(T) with 98.3% and 98.1% similarity (16S rRNA gene), respectively, and 84.4% similarity with Acinetobacter nectaris SAP 763.2(T) (rpoB gene). The major cellular fatty acids were summed features 3 (comprising C16:1ω7c /C16:1ω6c ), C12:0 and C16:0. The main isoprenoid quinone was ubiquinone-9 (Q-9). The polar lipids of strain HYN18(T) were phosphatidylethanolamine, three unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 40.6 mol%. DNA-DNA hybridization experiments indicated less than 33 ± 10% relatedness to the closest phylogenetic species, Acinetobacter nectaris SAP 763.2(T). Thus, the phenotypic, phylogenetic and genotypic analyses indicate that strain HYN18(T) is a novel species within the genus Acinetobacter, for which the name Acinetobacter apis is proposed. The type strain is HYN18(T) (=KACC 16906(T) =JCM 18575(T)).

  10. Taponamiento cardíaco secundario a carcinoma papilar esclerosante difuso de tiroides

    Directory of Open Access Journals (Sweden)

    Verónica Riva

    2011-12-01

    Full Text Available El carcinoma papilar, variante esclerosante difusa, corresponde al 2% de todos los carcinomas papilares de la tiroides. Se caracteriza por comprometer de manera difusa y bilateral a la glándula tiroides. Clínicamente se manifiesta con metástasis ganglionares y pulmonares, afectando predominantemente a mujeres jóvenes. Se describe un caso de taponamiento cardíaco como presentación inicial de un carcinoma papilar de tiroides variante esclerosante difusa. Una mujer de 32 años concurrió al servicio de emergencias médicas refiriendo epigastralgia y tos seca. Durante el examen físico se constató hipotensión arterial, taquicardia y ruidos cardíacos disminuidos. Se realizó un ecocardiograma, observándose derrame pericárdico. Por medio de una pericardiocentesis se obtuvo líquido pericárdico, cuyo análisis mostró células neoplásicas. Durante la evolución la paciente presentó recurrencia del derrame pericárdico por lo que se realizó una ventana pleuropericárdica, detectándose durante la cirugía una lesión nodular subpleural, la cual fue biopsiada e informada posteriormente como una metástasis de carcinoma papilar vinculable a origen tiroideo. Se realizó una tiroidectomía total con linfadenectomía cervical bilateral. El diagnóstico final fue carcinoma papilar, variante esclerosante difusa. Esta variante infiltra el tejido conectivo de los espacios interfoliculares, simulando una tiroiditis y se caracteriza por una permeación vascular temprana. En oposición a la variante clásica, la esclerosante difusa presenta mayor agresividad y mayor tasa de recurrencia. El carcinoma papilar de tiroides debe tenerse presente como diagnóstico diferencial en nuestro medio, en todas aquellas lesiones neoplásicas papilares metastásicas, más aún si se trata de mujeres jóvenes.

  11. 基于多蚁群的并行ACO算法%Parallel ACO Algorithm Based on Multiple Ant Colony

    Institute of Scientific and Technical Information of China (English)

    夏鸿斌; 须文波; 刘渊

    2009-01-01

    This paper proposes and implements a new approach to parallel Ant Colony Optimization(ACO) algorithms by changing the behavior of ACO. In view of the shortcomings for ant algorithms' stagnant, by improving selection strategies, a new selection and search strategies with parallel adaptive mechanisms are implemented, so as to strengthen its global search capability, and the method of data parallel is used to reduce communication time between processors and get a better solution. The performance of the proposed parallel algorithm, applied to the Traveling Salesman Problem(TSP), is investigated and evaluated with respect to solution quality and computational effort. Experimental results demonstrate that the proposed algorithm outperforms the sequential ant colony system as well as the existing parallel ACO algorithms.%通过改变蚁群优化(ACO)算法行为,提出一种新的ACO并行化策略--并行多蚁群ACO算法.针对蚁群算法存在停滞现象的缺点,改进选择策略,实现具有自适应并行机制的选择和搜索策略,以加强其全局搜索能力.并行处理采用数据并行的手段,能减少处理器间的通信时间并获得更好的解.以对称TSP测试集为对象进行比较实验,结果表明,该算法相对于串行算法及现有的并行算法具有一定的优势.

  12. An integrated ACO approach for the joint production and preventive maintenance scheduling problem in the flowshop sequencing problem.

    OpenAIRE

    Benbouzid-Sitayeb, Fatima; Ammi, Ismaïl; Varnier, Christophe; Zerhouni, Noureddine

    2008-01-01

    International audience; In this paper, an integrated ACO approach to solve joint production and preventive maintenance scheduling problem in permutation flowshops is considered. A newly developed antcolony algorithm is proposed and analyzed for solving this problem, based on a common representation of production and maintenance data, to obtain a joint schedule that is, subsequently, improved by a new local search procedure. The goal is to optimize a common objective function which takes into ...

  13. The Norma cluster (ACO3627): II. The near infrared K_s-band luminosity function

    CERN Document Server

    Skelton, R E; Kraan-Korteweg, R C

    2009-01-01

    A deep K_s-band photometric catalogue of galaxies at the core of the rich, nearby Norma cluster (ACO3627) is presented. The survey covers about 45 by 45 square arcmin (slightly less than 1/3 Abell radius), which corresponds to approx. 0.8 Mpc^2 at the adopted distance (v_cmb/H0) of 70 Mpc of this cluster. The survey is estimated to be complete to a magnitude of M_Ks <~ -19.5 mag. This extends into the dwarf regime, 6 magnitudes below M_Ks*. The catalogue contains 390 objects, 235 of which are classified as likely or definite galaxies and 155 as candidate galaxies. The Ks-band luminosity function (LF) is constructed from the photometric sample, using a spectroscopic subsample to correct for fore- and background contamination. We fit a Schechter function with a characteristic magnitude of M_Ks* = -25.39 \\pm 0.80 mag and faint-end slope of alpha = -1.26 \\pm 0.10 to the data. The shape of the LF is similar to those found in previous determinations of the cluster LF, in both optical and near infrared. The Schec...

  14. NASA GES DISC support of CO2 Data from OCO-2, ACOS, and AIRS

    Science.gov (United States)

    Wei, Jennifer C; Vollmer, Bruce E.; Savtchenko, Andrey K.; Hearty, Thomas J; Albayrak, Rustem Arif; Deshong, Barbara E.

    2013-01-01

    NASA Goddard Earth Sciences Data and Information Services Centers (GES DISC) is the data center assigned to archive and distribute current AIRS, ACOS data and data from the upcoming OCO-2 mission. The GES DISC archives and supports data containing information on CO2 as well as other atmospheric composition, atmospheric dynamics, modeling and precipitation. Along with the data stewardship, an important mission of GES DISC is to facilitate access to and enhance the usability of data as well as to broaden the user base. GES DISC strives to promote the awareness of science content and novelty of the data by working with Science Team members and releasing news articles as appropriate. Analysis of events that are of interest to the general public, and that help in understanding the goals of NASA Earth Observing missions, have been among most popular practices.Users have unrestricted access to a user-friendly search interface, Mirador, that allows temporal, spatial, keyword and event searches, as well as an ontology-driven drill down. Variable subsetting, format conversion, quality screening, and quick browse, are among the services available in Mirador. The majority of the GES DISC data are also accessible through OPeNDAP (Open-source Project for a Network Data Access Protocol) and WMS (Web Map Service). These services add more options for specialized subsetting, format conversion, image viewing and contributing to data interoperability.

  15. Bloqueio cardíaco completo em espondilite anquilosante Complete heart block in ankylosing spondylitis

    Directory of Open Access Journals (Sweden)

    Juan Pablo Restrepo

    2012-10-01

    Full Text Available A espondilite anquilosante é uma doença reumática crônica de homens jovens que afeta principalmente o esqueleto axial e está associada ao HLA-B27 em 90% dos casos. A incidência de envolvimento cardiovascular em casos de espondilite anquilosante varia entre 10%-30%; foram descritos distúrbios de condução em 1%-9% dos pacientes com a doença. A maior parte do grupo acometido demonstra uma relação com doença de longa data. Este é o primeiro relato de bloqueio cardíaco completo em espondilite anquilosante precoce de nosso conhecimento.Ankylosing spondylitis (AS is a chronic rheumatic disease of young men that affects mainly the axial skeleton and is associated with HLA-B27 in 90% of the cases. Incidence of cardiovascular involvement in AS ranges between 10%-30%; conduction disturbances have been described in 1%-9% of the patients with AS. The majority of the series show a relationship with longstanding disease. To our knowledge, this is the first report of complete heart block in early AS.

  16. ACO-zeotype iron aluminum phosphates with variable Al/Fe ratios controlled by F⁻ ions.

    Science.gov (United States)

    Wang, Yanyan; Li, Yi; Wang, Lei; Zhang, Jingzhe; Yan, Yan; Li, Jiyang; Yu, Jihong; Wang, Jincheng; Xu, Ruren

    2011-03-01

    Three new iron aluminum phosphates |(C(2)H(10)N(2))(4)|[Fe(8 - x)Al(x)F(x)(H(2)O)(2 - x)(PO(4))(8)]·2H(2)O (χ = 1.64, 1.33, 0.80) with ACO-zeotype structures denoted as FeAPO-CJ66(a), FeAPO-CJ66(b), and FeAPO-CJ66(c), respectively, have been synthesized in the fluoride ion system. Their framework structures are made of double 4-ring (D4R) building units formed by the alternating connection of Fe(Al)O(4)F(O) trigonal bipyramids and PO(4) tetrahedra, which possess 3D intersecting 8-ring channels running along the [001], [010], and [100] directions. Fluoride ions or water molecules reside in the center of D4Rs, and diprotonated ethylenediamine cations and water molecules are occluded in the free space of channels to stabilize the whole structure. Notably, the Al/Fe ratios in the frameworks can be effectively controlled from 1/3.9 to 1/5.0 to 1/9.0 by adjusting the amounts of phosphoric acid and hydrofluoric acid added to the initial reaction mixture. Mössbauer and magnetic measurements show that the Fe ions in the compounds are bivalent and undergo antiferromagnetic ordering at room temperature.

  17. REGISTRO DE PARO CARDÍACO EN EL ADULTO Registries of outer hospital cardiac arrest in Bogotta-Colombia

    Directory of Open Access Journals (Sweden)

    Ricardo Navarro Vargas

    2005-07-01

    Full Text Available En nuestro país no hay un registro estandarizado de los eventos cardíacos que requieren reanimación cerebro-cardio-pulmonar (RCCP siguiendo los lineamientos "Utstein". El propósito de este estudio fue determinar la calidad de los registros extrahospitalarios de paro cardíaco en el adulto, en Bogotá-Colombia. Entre enero y marzo de 2005 se realizó un análisis retrospectivo de los casos que correspondieron a paro cardíaco en el adulto atendidos por el "Centro Regulador de Urgencias del Distrito (CRU". El estudio reveló un registro deficiente de los eventos que requirieron RCCP realizados a nivel prehospitalario, e identificó al trauma como causa de paro cardíaco en 22% de los casos, una cifra particularmente elevada si se compara con los reportes mundiales, que oscilan entre 5% y 8%. Recomienda la estandarización del registro de paro cardíaco en el adulto, para lo cual se sugiere utilizar el formato de registro que sigue los lineamientos "Utstein".There is not a standardized registry form of cardiac events requiring cardiopulmonary resuscitation (CPR in our country such as the Utstein style. The purpose of this study was to determine the quality of the registries of out-ofhospital cardiac arrests in Bogotá-Colombia. A retrospective survey of registries of cardiac events assisted by a regulatory center "Centro Regulador de Urgencias del Distrito (CRU" was conducted, between January and March, 2005. The study reveals a poor registry of outof-hospital cardiac arrest in Bogotá-Colombia, and identifies trauma as the cause of cardiac arrest in 22% of cases, a particularly high figure compared with 5-8% registered worldwide. Recommend the standardization of the registry form of out-of-hospital cardiac arrest and suggest to use the "uniform reporting of data following cardiac arrest - the Utstein style". The registry forms are shown in appendix 1-2. Suggestions are welcome.

  18. Sperm use economy of honeybee (Apis mellifera) queens

    DEFF Research Database (Denmark)

    Baer, Boris; Collins, Jason; Maalaps, Kristiina;

    2016-01-01

    the fecundity and longevity of queens and therefore colony fitness. We quantified the number of sperm that honeybee (Apis mellifera) queens use to fertilize eggs. We examined sperm use in naturally mated queens of different ages and in queens artificially inseminated with different volumes of semen. We found...

  19. Standard methods for research on apis mellifera gut symbionts

    Science.gov (United States)

    Gut microbes can play an important role in digestion, disease resistance, and the general health of animals, but little is known about the biology of gut symbionts in Apis mellifera. This paper is part of a series on honey bee research methods, providing protocols for studying gut symbionts. We desc...

  20. The Proteins API: accessing key integrated protein and genome information.

    Science.gov (United States)

    Nightingale, Andrew; Antunes, Ricardo; Alpi, Emanuele; Bursteinas, Borisas; Gonzales, Leonardo; Liu, Wudong; Luo, Jie; Qi, Guoying; Turner, Edd; Martin, Maria

    2017-04-05

    The Proteins API provides searching and programmatic access to protein and associated genomics data such as curated protein sequence positional annotations from UniProtKB, as well as mapped variation and proteomics data from large scale data sources (LSS). Using the coordinates service, researchers are able to retrieve the genomic sequence coordinates for proteins in UniProtKB. This, the LSS genomics and proteomics data for UniProt proteins is programmatically only available through this service. A Swagger UI has been implemented to provide documentation, an interface for users, with little or no programming experience, to 'talk' to the services to quickly and easily formulate queries with the services and obtain dynamically generated source code for popular programming languages, such as Java, Perl, Python and Ruby. Search results are returned as standard JSON, XML or GFF data objects. The Proteins API is a scalable, reliable, fast, easy to use RESTful services that provides a broad protein information resource for users to ask questions based upon their field of expertise and allowing them to gain an integrated overview of protein annotations available to aid their knowledge gain on proteins in biological processes. The Proteins API is available at (http://www.ebi.ac.uk/proteins/api/doc).

  1. Fertile diploid drones in africanized honeybees, Apis mellifera adansonii.

    Science.gov (United States)

    Chaud-Netto, J

    1977-02-15

    59 diploid drones of Apis mellifera adansonii, 12-37 days old, were tested for the presence of semen after provoked ejaculation; 13 drones ejaculated semen enough to be used in an instrumental insemination, but only three on them (5%) furnished 1 mm3 of semen. The problems referring to the attainment of descendants from the 2n drones are briefly discussed.

  2. The MPO API: A tool for recording scientific workflows

    Energy Technology Data Exchange (ETDEWEB)

    Wright, John C., E-mail: jcwright@mit.edu [MIT Plasma Science and Fusion Center, Cambridge, MA (United States); Greenwald, Martin; Stillerman, Joshua [MIT Plasma Science and Fusion Center, Cambridge, MA (United States); Abla, Gheni; Chanthavong, Bobby; Flanagan, Sean; Schissel, David; Lee, Xia [General Atomics, San Diego, CA (United States); Romosan, Alex; Shoshani, Arie [Lawrence Berkeley Laboratory, Berkeley, CA (United States)

    2014-05-15

    Highlights: • A description of a new framework and tool for recording scientific workflows, especially those resulting from simulation and analysis. • An explanation of the underlying technologies used to implement this web based tool. • Several examples of using the tool. - Abstract: Data from large-scale experiments and extreme-scale computing is expensive to produce and may be used for high-consequence applications. The Metadata, Provenance and Ontology (MPO) project builds on previous work [M. Greenwald, Fusion Eng. Des. 87 (2012) 2205–2208] and is focused on providing documentation of workflows, data provenance and the ability to data-mine large sets of results. While there are important design and development aspects to the data structures and user interfaces, we concern ourselves in this paper with the application programming interface (API) – the set of functions that interface with the data server. Our approach for the data server is to follow the Representational State Transfer (RESTful) software architecture style for client–server communication. At its core, the API uses the POST and GET methods of the HTTP protocol to transfer workflow information in message bodies to targets specified in the URL to and from the database via a web server. Higher level API calls are built upon this core API. This design facilitates implementation on different platforms and in different languages and is robust to changes in the underlying technologies used. The command line client implementation can communicate with the data server from any machine with HTTP access.

  3. PrismTech Data Distribution Service Java API Evaluation

    Science.gov (United States)

    Riggs, Cortney

    2008-01-01

    My internship duties with Launch Control Systems required me to start performance testing of an Object Management Group's (OMG) Data Distribution Service (DDS) specification implementation by PrismTech Limited through the Java programming language application programming interface (API). DDS is a networking middleware for Real-Time Data Distribution. The performance testing involves latency, redundant publishers, extended duration, redundant failover, and read performance. Time constraints allowed only for a data throughput test. I have designed the testing applications to perform all performance tests when time is allowed. Performance evaluation data such as megabits per second and central processing unit (CPU) time consumption were not easily attainable through the Java programming language; they required new methods and classes created in the test applications. Evaluation of this product showed the rate that data can be sent across the network. Performance rates are better on Linux platforms than AIX and Sun platforms. Compared to previous C++ programming language API, the performance evaluation also shows the language differences for the implementation. The Java API of the DDS has a lower throughput performance than the C++ API.

  4. The Automatic Start Method of Application Program Using API

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    This paper introduces on a method about the au-tomactic start of application program. Through defining Registryby API function, the automatic start of specified application pro-gram is fulfilled when Windows98 is taking action. It gives facil-ities to many computer application works.

  5. Modelling the subgenual organ of the honeybee, Apis mellifera

    DEFF Research Database (Denmark)

    Storm, Jesper; Kilpinen, Ole

    1998-01-01

    In a recent study on the honeybee (Apis mellifera), the subgenual organ was observed moving inside the leg during sinusoidal vibrations of the leg (Kilpinen and Storm 1997). The subgenual organ of the honeybee is suspended in a haemolymph channel in the tibia of each leg. When the leg accelerates...

  6. EXPERIENCE API – NEW STANDARD OF E-LEARNING SOFTWARE AND EXAMPLES OF ITS PRACTICAL USE

    Directory of Open Access Journals (Sweden)

    Oleksandr A. Shcherbyna

    2016-07-01

    Full Text Available The purpose of the article is to analyze features of the new standard of e-learning software - Experience API (xAPI, previously also known as the Tin Can API. The standard defines a way of interaction between xAPI-clients – software which students work with while e-learning process, and xAPI-servers – Learning Record Stores (LRS, which store data about their results. Standard also defines LRS data representation format and a way of data transfers between LRS, which makes it possible to combine several LRS into distributed database that could accumulate information about people training in formal, non-formal and informal education throughout life. The article contains review of available xAPI-clients, xAPI-servers, and the results of their testing, which prove the possibility of their usage in our educational institutions.

  7. Host Specificity in the Honeybee Parasitic Mite, Varroa spp. in Apis mellifera and Apis cerana.

    Directory of Open Access Journals (Sweden)

    Alexis L Beaurepaire

    Full Text Available The ectoparasitic mite Varroa destructor is a major global threat to the Western honeybee Apis mellifera. This mite was originally a parasite of A. cerana in Asia but managed to spill over into colonies of A. mellifera which had been introduced to this continent for honey production. To date, only two almost clonal types of V. destructor from Korea and Japan have been detected in A. mellifera colonies. However, since both A. mellifera and A. cerana colonies are kept in close proximity throughout Asia, not only new spill overs but also spill backs of highly virulent types may be possible, with unpredictable consequences for both honeybee species. We studied the dispersal and hybridisation potential of Varroa from sympatric colonies of the two hosts in Northern Vietnam and the Philippines using mitochondrial and microsatellite DNA markers. We found a very distinct mtDNA haplotype equally invading both A. mellifera and A. cerana in the Philippines. In contrast, we observed a complete reproductive isolation of various Vietnamese Varroa populations in A. mellifera and A. cerana colonies even if kept in the same apiaries. In light of this variance in host specificity, the adaptation of the mite to its hosts seems to have generated much more genetic diversity than previously recognised and the Varroa species complex may include substantial cryptic speciation.

  8. Changes in Alternative Splicing in Apis Mellifera Bees Fed Apis Cerana Royal Jelly

    Directory of Open Access Journals (Sweden)

    Shi Yuan Yuan

    2014-12-01

    Full Text Available The Western honey bee (Apis mellifera is a social insect characterized by caste differentiation in which the queen bee and worker bees display marked differences in morphology, behavior, reproduction, and longevity despite their identical genomes. The main causative factor in caste differentiation is the food fed to queen larvae, termed royal jelly (RJ. Alternative splicing (AS is an important RNA-mediated post-transcriptional process in eukaryotes. Here we report AS changes in A. mellifera after being fed either A. mellifera RJ or A. cerana RJ. The results demonstrated that the RJ type affected 4 types of AS in adult A. mellifera: exon skipping, intron retention, alternative 5’ splice sites, and alternative 3’splice sites. After feeding with A. cerana RJ, AS occurred in many genes in adult A. mellifera that encode proteins involved in development, growth, the tricarboxylic acid cycle, and substance metabolism. This study provides the first evidence that heterospecific RJ can influence the AS of many genes related to honey bee development and growth.

  9. Host Specificity in the Honeybee Parasitic Mite, Varroa spp. in Apis mellifera and Apis cerana.

    Science.gov (United States)

    Beaurepaire, Alexis L; Truong, Tuan A; Fajardo, Alejandro C; Dinh, Tam Q; Cervancia, Cleofas; Moritz, Robin F A

    2015-01-01

    The ectoparasitic mite Varroa destructor is a major global threat to the Western honeybee Apis mellifera. This mite was originally a parasite of A. cerana in Asia but managed to spill over into colonies of A. mellifera which had been introduced to this continent for honey production. To date, only two almost clonal types of V. destructor from Korea and Japan have been detected in A. mellifera colonies. However, since both A. mellifera and A. cerana colonies are kept in close proximity throughout Asia, not only new spill overs but also spill backs of highly virulent types may be possible, with unpredictable consequences for both honeybee species. We studied the dispersal and hybridisation potential of Varroa from sympatric colonies of the two hosts in Northern Vietnam and the Philippines using mitochondrial and microsatellite DNA markers. We found a very distinct mtDNA haplotype equally invading both A. mellifera and A. cerana in the Philippines. In contrast, we observed a complete reproductive isolation of various Vietnamese Varroa populations in A. mellifera and A. cerana colonies even if kept in the same apiaries. In light of this variance in host specificity, the adaptation of the mite to its hosts seems to have generated much more genetic diversity than previously recognised and the Varroa species complex may include substantial cryptic speciation.

  10. Quality of life profile and psychometric properties of the EQ-5D-5L in HIV/AIDS patients

    Directory of Open Access Journals (Sweden)

    Tran Bach

    2012-11-01

    Full Text Available Abstract Objectives We assessed health-related quality of life (HRQOL, its associated factors, and examined measurement properties of the EuroQol - 5 Dimensions - 5 Levels (EQ-5D-5L in HIV/AIDS patients. Methods A cross-sectional multi-site survey was conducted in 1016 patients (age: 35.4 ± 7.0 years; 63.8% male in three epicenters of Vietnam. Internal consistency reliability, convergent validity, and discriminative validity of the EQ-5D-5L and a visual analogue scale (VAS were evaluated. Tobit censored regression models were used to identify predictors of HRQOL in HIV/AIDS patients. Results The mean EQ-5D-5L single index and VAS were 0.65 (95% Confidence Interval (CI = 0.63; 0.67 and 70.3 (95% CI = 69.2; 71.5. Cronbach’s alpha of five dimensions was 0.85. EQ-5D-5L has a good convergent validity with VAS (0.73. It discriminated patients at different HIV/AIDS stages, duration of ART, and CD4 cell count. Predictors of poorer HRQOL included being female, lower education level, unemployment, alcohol and drug use, CD4 Conclusion The EQ-5D-5L has good measurement properties in HIV/AIDS patients and holds potentials for monitoring ART outcomes. Integration of HRQOL measurement using EQ-5D-5L in HIV/AIDS clinical practice could be helpful for economic evaluation of HIV/AIDS interventions.

  11. Adhesions of extracellular surface-layer associated proteins in Lactobacillus M5-L and Q8-L.

    Science.gov (United States)

    Zhang, Yingchun; Xiang, Xinling; Lu, Qianhui; Zhang, Lanwei; Ma, Fang; Wang, Linlin

    2016-02-01

    Surface-layer associated proteins (SLAP) that envelop Lactobacillus paracasei ssp. paracasei M5-L and Lactobacillus casei Q8-L cell surfaces are involved in the adherence of these strain to the human intestinal cell line HT-29. To further elucidate some of the properties of these proteins, we assessed the yields and expressions of SLAP under different incubation conditions. An efficient and selective extraction of SLAP was obtained when cells of Lactobacillus were treated with 5 M LiCl at 37°C in aerobic conditions. The SLAP of Lactobacillus M5-L and Q8-L in cell extracts were visualized by SDS-PAGE and identified by Western blotting with sulfo-N-hydroxysuccinimide-biotin-labeled HT-29 cells as adhesion proteins. Atomic force microscopy contact imaging revealed that Lactobacillus strains M5-L and Q8-L normally display a smooth, homogeneous surface, whereas the surfaces of M5-L and Q8-L treated with 5 M LiCl were rough and more heterogeneous. Analysis of adhesion forces revealed that the initial adhesion forces of 1.41 and 1.28 nN obtained for normal Lactobacillus M5-L and Q8-L strains, respectively, decreased to 0.70 and 0.48 nN, respectively, following 5 M LiCl treatment. Finally, the dominant 45-kDa protein bands of Lactobacillus Q8-L and Lactobacillus M5-L were identified as elongation factor Tu and surface antigen, respectively, by liquid chromatography-tandem mass spectrometry.

  12. Transplante cardíaco humano: experiência inicial Human cardiac transplant: initial experience

    Directory of Open Access Journals (Sweden)

    Noedir A. G Stolf

    1986-12-01

    Full Text Available No Instituto do Coração, de março de 1985 a fevereiro de 1986,11 pacientes foram submetidos a transplante cardíaco ortotópico. Eram todos do sexo masculino, com idade variando de 39 a 54 anos; 6 com cardiopatia isquémica, 4 com cardiomiopatia dilatada e um com cardiomiopatia chagásica. Foi realizado estudo hemodinâmico através de catéter de Swan-Ganz, no pré-operatório, no pós-operatório, após estabilização na unidade de recuperação, e trinta ou mais dias após o transplante. Os dados mostram melhora progressiva em relação ao índice cardíaco, pressão em artéria pulmonar, pressão de capilar pulmonar, resistência vascular pulmonar e resistência vascular sistêmica. Três dos 11 pacientes apresentaram disfunção renal transitória no pós-operatório imediato e que regrediram até o 15º dia, enquanto que 2 pacientes apresentaram aumento moderado da creatinina plasmática. Apenas 3 pacientes não apresentaram qualquer episódio de rejeição; nos demais, esses episódios foram um diagnóstico histológico sem repercussões clínicas. Complicações infecciosas ocorreram em 9 pacientes e foram de fácil controle clínico. No pós-operatório tardio, a hipertensão esteve presente em 8 pacientes, sendo mais acentuada em 2 deles. Não houve óbitos, nesta série de pacientes; todos estão assintomáticos e os 6 primeiros estão trabalhando.At the Instituto do Cora��ão, University of São Paulo Medical School, 11 patients were submitted to heart transplantation from march 1985 up to february 1986. All were male, with ages of 39-59 years, 6 with coronary heart disease, 4 with dilated cardiomyopathy and 1 with Chagas cardiomyopathy. The patients were studied hemodynamically with a Swan-Ganz catheter pre-operatively, at the arrival in the intensive care unit, in the first postoperative day and 30 or more days after the transplant. The data showed that there was a progressive increase of cardiac index and decreases of

  13. Tumores cardíacos: 10 anos de experiência

    Directory of Open Access Journals (Sweden)

    Antônio Augusto MIANA

    1997-01-01

    Full Text Available Objetivo: Avaliar a experiência cirúrgica do grupo no tratamento dos tumores cardíacos durante o período de janeiro de 1985 a dezembro de 1994. Casuística e Métodos: De um total de 2268 cirurgias cardíacas com circulação extracorpórea realizadas neste período de 10 anos, 6 foram para extirpação de tumores intracavitários, perfazendo 0,26% dos casos. Destes, 3 eram mixomas de átrio esquerdo, 1 fibroelastoma papilar de valva mitral, 1 rabdomioma de ventrículo esquerdo e 1 fibroma de ventrículo esquerdo. A forma clínica de apresentação foi embolia sistêmica (3 casos ou insuficiência cardíaca (3 casos. O diagnóstico foi ecocardiográfico (5 casos e angiográfico (6 casos. Resultados: Todos os pacientes tiveram evolução imediata favorável, exceto 1 deles, com mixoma de átrio equerdo, que evoluiu com mediastinite e septicemia, vindo a falecer e constituindo o único óbito hospitalar. O seguimento pós-operatório tardio, obtido em 4 pacientes (15 a 111 meses - média 49 ± 36,8 meses, não detectou qualquer recidiva, encontrando-se todos em classe funcional I (NYHA. Conclusões: Os autores concluem que os tumores cardíacos são bastante raros, de fácil diagnóstico desde que considerados, na grande maioria benignos e que cursam favoravelmente com a extirpação cirúrgica.Purpose: To review the surgical experience of our group in the treatment of primary cardiac tumors, during a 10 year period beginning January 1985 till December 1994. Material and Methods: From a total of 2268 cardiac surgeries with the aid of extracorporeal circulation performed during this 10 year period, there were 6 cases of intracavitary tumor resection, an incidence of 0.26%. Three were myxomas of the left atrium, 1 papillary fibroelastoma of the mitral valve, 1 rhabdomyoma of the left ventricule and 1 fibroma of the left ventricle. Three patients presented systemic embolism and the other three congestive heart failure. The diagnosis was confirmed

  14. Exo-Arabinanase of Penicillium chrysogenum Able To Release Arabinobiose from α-1,5-l-Arabinan

    OpenAIRE

    Sakamoto, Tatsuji; Thibault, Jean-François

    2001-01-01

    An exo-arabinanase, designated Abnx, was purified from a culture filtrate of Penicillium chrysogenum 31B by ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic chromatography. Abnx had an apparent molecular mass of 47 kDa. The enzyme released only arabinobiose from the nonreducing terminus of α-1,5-l-arabinan and showed no activity towards p-nitrophenyl-α-l-arabinofuranoside and α-1,5-l-arabinofuranobiose. Abnx is the first enzyme with this mode of action.

  15. Changes in oxidative stress in transgenic RNAi ACO1 tomato fruit during ripening

    Science.gov (United States)

    Eglous, Najat Mohamed; Ali, Zainon Mohd; Hassan, Maizom; Zainal, Zamri

    2013-11-01

    Tomato (Solanum Lycopersicum L.) is the second most cultivated vegetable in the world and widely used as a system for studying the role of ethylene during fruit ripening. Our objective was to study the oxidative stress and antioxidative metabolism during ripening of non transgenic tomato and transgenic line-21 tomato which reduced ethylene. The line-21 of transgenic tomato plants (RNAi ACO1) had lower ethylene production and longer shelf-life more than 32 days as compared to the wild-type fruits which have very short shelf-life. In this study, tomato fruit were divided into five different stages (MG: mature green 5%, B: breaker 25%, T: turning 50%, O: orange75%, RR: red ripe100%). The activity of lipoxygenase (LOX) and lipid peroxidation (MDA) were measured to assess changes in oxidative stress. The LOX activity and MDA content decreased significantly obtaining 2.6-fold and 1.2-fold, respectively, as compared to the wild type fruit. However, superoxide dismutase (SOD) and catalase (CAT) activities were increased to 1.9 and 1.2 folds from the mature green to the fully ripe stage in transgenic tomatoes. Furthermore, the wild type tomato increases 1.3 in SOD and 1.6 in CAT activities. The overall results indicate that the wild type tomato fruit showed a faster rate of ripening, parallel to decline in the rate of enzymatic antioxidative systems as compared to the transgenic line-21 tomato fruit. In addition, the results show that the antioxidant capacity is improved during the ripening process and is accompanied by an increase in the oxidative stress.

  16. Qualification and Certification Requirements of Steel Tube Nondestructive Testing Personnel in API Product Standards%产品标准中钢管无损检测人员资格鉴定要求

    Institute of Scientific and Technical Information of China (English)

    张黎; 赵仁顺

    2016-01-01

    依据API SPEC 5 CT «套管和油管规范»和 API SPEC 5 L «管线管规范»标准对无损检测(NDT)人员资格的要求,对 ISO 9712:2012«无损检测———人员资格鉴定与认证»标准和 ISO 11484:2009«钢材产品———雇主的无损检测人员资格制度»标准的异同进行了分析,分析认为,两个标准规定的无损检测方法、无损检测人员资格鉴定等级和对雇主职责的要求不同,但对无损检测人员的视力要求、无损检测人员的操作授权及资格证书有效期的规定是一致的。%According to the nondestructive testing (NDT)personnel qualification requirements specified by API Spec 5CT and API Spec 5L,an analysis was made of the similarities and differences between the standards of ISO 9712:2012 and ISO 11484:2009.The analysis concluded that the two standards showed differences in NDT methods,nondestructive testing personnel qualification level and responsibilities requirement for employers,but they had the same requirement for the vision of NDT personnel,nondestructive testing personnel operating licenses and the valid period of time of qualification certificate.

  17. SmartPATH: An Efficient Hybrid ACO-GA Algorithm for Solving the Global Path Planning Problem of Mobile Robots

    Directory of Open Access Journals (Sweden)

    Imen Châari

    2014-07-01

    Full Text Available Path planning is a fundamental optimization problem that is crucial for the navigation of a mobile robot. Among the vast array of optimization approaches, we focus in this paper on Ant Colony Optimization (ACO and Genetic Algorithms (GA for solving the global path planning problem in a static environment, considering their effectiveness in solving such a problem. Our objective is to design an efficient hybrid algorithm that takes profit of the advantages of both ACO and GA approaches for the sake of maximizing the chance to find the optimal path even under real-time constraints. In this paper, we present smartPATH, a new hybrid ACO-GA algorithm that relies on the combination of an improved ACO algorithm (IACO for efficient and fast path selection, and a modified crossover operator to reduce the risk of falling into a local minimum. We demonstrate through extensive simulations that smartPATH outperforms classical ACO (CACO, GA algorithms. It also outperforms the Dijkstra exact method in solving the path planning problem for large graph environments. It improves the solution quality up to 57% in comparison with CACO and reduces the execution time up to 83% as compared to Dijkstra for large and dense graphs. In addition, the experimental results on a real robot shows that smartPATH finds the optimal path with a probability up to 80% with a small gap not exceeding 1m in 98%.

  18. Crystallization and preliminary crystallographic analysis of exo-α-1,5-l-arabinofuranosidase from Streptomyces avermitilis NBRC14893

    OpenAIRE

    2008-01-01

    Exo-α-1,5-l-arabinofuranosidase from S. avermitilis NBRC14893 was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 2.2 Å and belonged to space group P212121.

  19. Sildenafil vs. Nitroprussiato de Sódio durante Teste de Reatividade Pulmonar pré-transplante cardíaco

    OpenAIRE

    Freitas Jr, Aguinaldo Figueiredo; Bacal, Fernando; Oliveira Júnior, José de Lima; Fiorelli, Alfredo Inácio; Santos, Ronaldo Honorato; Moreira, Luiz Felipe Pinho; Silva, Christiano Pereira; Mangini, Sandrigo; Tsutsui, Jeane Mike; Bocchi, Edimar Alcides

    2012-01-01

    FUNDAMENTO: A hipertensão pulmonar é associada ao pior prognóstico no pós-transplante cardíaco. O teste de reatividade pulmonar com Nitroprussiato de Sódio (NPS) está associado a elevados índices de hipotensão arterial sistêmica, disfunção ventricular do enxerto transplantado e elevadas taxas de desqualificação para o transplante. OBJETIVO: Neste estudo, objetivou-se comparar os efeitos do Sildenafil (SIL) e NPS sobre variáveis hemodinâmicas, neuro-hormonais e ecocardiográficas durante teste ...

  20. Sedação com sufentanil e clonidina em pacientes submetidos a cateterismo cardíaco

    Directory of Open Access Journals (Sweden)

    Anita Perpetua Carvalho Rocha

    2011-03-01

    Full Text Available FUNDAMENTO: A sedação para a realização de cateterismo cardíaco tem sido alvo de preocupação. Benzodiazepínicos, agonistas alfa-2 adrenérgicos e opioides são utilizados para esse fim, entretanto, cada um destes medicamentos possui vantagens e desvantagens. OBJETIVO: Avaliar a eficácia do sufentanil e da clonidina como sedativos em pacientes submetidos a cateterismo cardíaco, observando o impacto dos mesmos sobre os parâmetros hemodinâmicos e respiratórios, a presença de efeitos colaterais, além da satisfação do paciente e do hemodinamicista com o exame. MÉTODOS: Trata-se de um ensaio clínico prospectivo, duplo-cego, randomizado e controlado, que envolveu 60 pacientes que receberam 0,1 µg/kg de sufentanil ou 0,5 µg/kg de clonidina antes da realização do cateterismo cardíaco. O escore de sedação segundo a escala de Ramsay, a necessidade de utilização de midazolam, os efeitos colaterais, os parâmetros hemodinâmicos e respiratórios foram registrados, sendo os dados analisados em 06 diferentes momentos. RESULTADOS: O comportamento da pressão arterial, da frequência cardíaca e da frequência respiratória foi semelhante nos dois grupos, entretanto, no momento 2, os pacientes do grupo sufentanil (Grupo S apresentaram menor escore de sedação segundo a escala de Ramsay, e a saturação periférica da oxihemoglobina foi menor que o grupo clonidina (Grupo C no momento 6. Os pacientes do Grupo S apresentaram maior incidência de náusea e vômito pós-operatório que os pacientes do Grupo C. A satisfação dos pacientes foi maior no grupo clonidina. Os hemodinamicistas mostraram-se satisfeitos nos dois grupos. CONCLUSÃO: O sufentanil e a clonidina foram efetivos como sedativos em pacientes submetidos a cateterismo cardíaco.

  1. Infra-population and -community dynamics of the parasites Nosema apis and Nosema ceranae, and consequences for honey bee (Apis mellifera hosts.

    Directory of Open Access Journals (Sweden)

    Geoffrey R Williams

    Full Text Available Nosema spp. fungal gut parasites are among myriad possible explanations for contemporary increased mortality of western honey bees (Apis mellifera, hereafter honey bee in many regions of the world. Invasive Nosema ceranae is particularly worrisome because some evidence suggests it has greater virulence than its congener N. apis. N. ceranae appears to have recently switched hosts from Asian honey bees (Apis cerana and now has a nearly global distribution in honey bees, apparently displacing N. apis. We examined parasite reproduction and effects of N. apis, N. ceranae, and mixed Nosema infections on honey bee hosts in laboratory experiments. Both infection intensity and honey bee mortality were significantly greater for N. ceranae than for N. apis or mixed infections; mixed infection resulted in mortality similar to N. apis parasitism and reduced spore intensity, possibly due to inter-specific competition. This is the first long-term laboratory study to demonstrate lethal consequences of N. apis and N. ceranae and mixed Nosema parasitism in honey bees, and suggests that differences in reproduction and intra-host competition may explain apparent heterogeneous exclusion of the historic parasite by the invasive species.

  2. “Wrapping” X3DOM around Web Audio API

    Directory of Open Access Journals (Sweden)

    Andreas Stamoulias

    2015-12-01

    Full Text Available Spatial sound has a conceptual role in the Web3D environments, due to highly realism scenes that can provide. Lately the efforts are concentrated on the extension of the X3D/ X3DOM through spatial sound attributes. This paper presents a novel method for the introduction of spatial sound components in the X3DOM framework, based on X3D specification and Web Audio API. The proposed method incorporates the introduction of enhanced sound nodes for X3DOM which are derived by the implementation of the X3D standard components, enriched with accessional features of Web Audio API. Moreover, several examples-scenarios developed for the evaluation of our approach. The implemented examples established the achievability of new registered nodes in X3DOM, for spatial sound characteristics in Web3D virtual worlds.

  3. The Auroral Planetary Imaging and Spectroscopy (APIS) service

    CERN Document Server

    Lamy, Laurent; Henry, Florence; Sidaner, Pierre Le

    2015-01-01

    The Auroral Planetary Imaging and Spectroscopy (APIS) service, accessible online, provides an open and interactive access to processed auroral observations of the outer planets and their satellites. Such observations are of interest for a wide community at the interface between planetology and magnetospheric and heliospheric physics. APIS consists of (i) a high level database, built from planetary auroral observations acquired by the Hubble Space Telescope (HST) since 1997 with its mostly used Far-UltraViolet spectro-imagers, (ii) a dedicated search interface aimed at browsing efficiently this database through relevant conditional search criteria and (iii) the ability to interactively work with the data online through plotting tools developed by the Virtual Observatory (VO) community, such as Aladin and Specview. This service is VO compliant and can therefore also been queried by external search tools of the VO community. The diversity of available data and the capability to sort them out by relevant physical...

  4. Draft genome sequence of the Algerian bee Apis mellifera intermissa.

    Science.gov (United States)

    Haddad, Nizar Jamal; Loucif-Ayad, Wahida; Adjlane, Noureddine; Saini, Deepti; Manchiganti, Rushiraj; Krishnamurthy, Venkatesh; AlShagoor, Banan; Batainh, Ahmed Mahmud; Mugasimangalam, Raja

    2015-06-01

    Apis mellifera intermissa is the native honeybee subspecies of Algeria. A. m. intermissa occurs in Tunisia, Algeria and Morocco, between the Atlas and the Mediterranean and Atlantic coasts. This bee is very important due to its high ability to adapt to great variations in climatic conditions and due to its preferable cleaning behavior. Here we report the draft genome sequence of this honey bee, its Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JSUV00000000. The 240-Mb genome is being annotated and analyzed. Comparison with the genome of other Apis mellifera sub-species promises to yield insights into the evolution of adaptations to high temperature and resistance to Varroa parasite infestation.

  5. Metadata and API Based Environment Aware Content Delivery Architecture

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    One of the limitations of current content delivery networks is lack of support for environment aware content delivery. This paper first discusses the requirements of such support, and proposes a new metadata gateway based environment aware content delivery architecture. The paper discusses in some details key functions and technologies of environment aware content delivery architecture, including its APIs and control policies. Finally the paper presents an application to illustrate advantages of environment aware content delivery architecture in the context of next generation network.

  6. Apis florea en Jordanie : origine de la population fondatrice

    OpenAIRE

    HADDAD, Nizar; FUCHS, Stefan; Randall Hepburn, H.; Radloff, Sarah E.

    2009-01-01

    International audience; A recent isolated population of Apis florea has been reported from Aqaba in Jordan at the Red Sea, consisting of numerous colonies within a still limited range which apparently is expanding. This region is about 1500 km apart from its next occurrences in Sudan where it had been introduced and first detected in 1985 and about 2000 km apart from its next natural occurrences in Iran and Oman. These bees apparently have been imported by human transport, most likely by ship...

  7. Measurement properties of the EQ-5D-5L compared to the EQ-5D-3L across eight patient groups

    DEFF Research Database (Denmark)

    Janssen, M F; Pickard, A Simon; Golicki, Dominik;

    2012-01-01

    ), redistribution properties, ceiling, discriminatory power, convergent validity, and known-groups validity. RESULTS: Missing values were on average 0.8 % for 5L and 1.3 % for 3L. In total, 2.9 % of responses were inconsistent between 5L and 3L. Redistribution from 3L to 5L using EQ dimension-specific rating scales......), and relative discriminatory power (Shannon Evenness index) improved slightly (mean 0.81 for 5L versus 0.78 for 3L). Convergent validity with WHO-5 was demonstrated and improved slightly with 5L. Known-groups validity was confirmed for both 5L and 3L. CONCLUSIONS: The EQ-5D-5L appears to be a valid extension...... of the 3-level system which improves upon the measurement properties, reducing the ceiling while improving discriminatory power and establishing convergent and known-groups validity....

  8. ADS 2.0: New Architecture, API and Services

    Science.gov (United States)

    Chyla, R.; Accomazzi, A.; Holachek, A.; Grant, C. S.; Elliott, J.; Henneken, E. A.; Thompson, D. M.; Kurtz, M. J.; Murray, S. S.; Sudilovsky, V.

    2015-09-01

    The ADS platform is undergoing the biggest rewrite of its 20-year history. While several components have been added to its architecture over the past couple of years, this talk will concentrate on the underpinnings of ADS's search layer and its API. To illustrate the design of the components in the new system, we will show how the new ADS user interface is built exclusively on top of the API using RESTful web services. Taking one step further, we will discuss how we plan to expose the treasure trove of information hosted by ADS (10 million records and fulltext for much of the Astronomy and Physics refereed literature) to partners interested in using this API. This will provide you (and your intelligent applications) with access to ADS's underlying data to enable the extraction of new knowledge and the ingestion of these results back into the ADS. Using this framework, researchers could run controlled experiments with content extraction, machine learning, natural language processing, etc. In this talk, we will discuss what is already implemented, what will be available soon, and where we are going next.

  9. Carbonic anhydrase from Apis mellifera: purification and inhibition by pesticides.

    Science.gov (United States)

    Soydan, Ercan; Güler, Ahmet; Bıyık, Selim; Şentürk, Murat; Supuran, Claudiu T; Ekinci, Deniz

    2017-12-01

    Carbonic anhydrase (CA) enzymes have been shown to play an important role in ion transport and in pH regulation in several organisms. Despite this information and the wealth of knowledge regarding the significance of CA enzymes, few studies have been reported about bee CA enzymes and the hazardous effects of chemicals. Using Apis mellifera as a model, this study aimed to determine the risk of pesticides on Apis mellifera Carbonic anhydrase enzyme (Am CA). CA was initially purified from Apis mellifera spermatheca for the first time in the literature. The enzyme was purified with an overall purification of ∼35-fold with a molecular weight of ∼32 kDa. The enzyme was then exposed to pesticides, including tebuconazole, propoxur, carbaryl, carbofuran, simazine and atrazine. The six pesticides dose-dependently inhibited in vitro AmCA activity at low micromolar concentrations. IC50 values for the pesticides were 0.0030, 0.0321, 0.0031, 0.0087, 0.0273 and 0.0165 μM, respectively. The AmCA inhibition mechanism of these compounds is unknown at this moment.

  10. Variation morphogeometrics of Africanized honey bees (Apis mellifera in Brazil

    Directory of Open Access Journals (Sweden)

    Lorena A. Nunes

    2012-09-01

    Full Text Available The morphometrics of the honey bee Apis mellifera L., 1758 has been widely studied mainly because this species has great ecological importance, high adaptation capacity, wide distribution and capacity to effectively adapt to different regions. The current study aimed to investigate the morphometric variations of wings and pollen baskets of honey bees Apis mellifera scutellata Lepeletier, 1836 from the five regions in Brazil. We used geometric morphometrics to identify the existence of patterns of variations of shape and size in Africanized honey bees in Brazil 16 years after the classic study with this species, allowing a temporal and spatial comparative analysis using new technological resources to assess morphometrical data. Samples were collected in 14 locations in Brazil, covering the five geographical regions of the country. The shape analysis and multivariate analyses of the wing allowed to observe that there is a geographical pattern among the population of Apis mellifera in Brazil. The geographical variations may be attributed to the large territorial extension of the country in addition to the differences between the bioregions.

  11. Estudio de la variabilidad del ritmo cardíaco mediante técnicas estadísticas, espectrales y no lineales

    OpenAIRE

    García González, Miguel Ángel

    1998-01-01

    El análisis de la variabilidad del ritmo cardíaco es una técnica no invasiva que permite la diagnosis y prognosis de cardiopatías y neuropatías. El ritmo cardíaco está modulado por diversos sistemas fisiológicos siendo el nexo de unión el sistema nervioso autónomo o vegetativo (tanto la rama parasimpática como la rama simpática). La degeneración del sistema nervioso autónomo o del tejido cardíaco queda reflejada en el análisis de la serie temporal RR mediante la cual se cuantifica y caracteri...

  12. Classificação de sons cardíacos usando Motifs: desenvolvimento de uma aplicação móvel

    OpenAIRE

    Oliveira, Soraia Carvalho da Cruz

    2014-01-01

    Este documento foi redigido no âmbito da Tese, do Mestrado em Engenharia Informática na área de Tecnologias do Conhecimento e Decisão, do Departamento de Engenharia Informática, do ISEP, cujo tema é classificação de sons cardíacos usando motifs. Neste trabalho, apresenta-se um algoritmo de classificação de sons cardíacos, capaz de identificar patologias cardíacas. A classificação do som cardíaco é um trabalho desafiante dada a dificuldade em separar os sons ambiente (vozes, respiração, con...

  13. Improving the API dissolution rate during pharmaceutical hot-melt extrusion I: Effect of the API particle size, and the co-rotating, twin-screw extruder screw configuration on the API dissolution rate.

    Science.gov (United States)

    Li, Meng; Gogos, Costas G; Ioannidis, Nicolas

    2015-01-15

    The dissolution rate of the active pharmaceutical ingredients in pharmaceutical hot-melt extrusion is the most critical elementary step during the extrusion of amorphous solid solutions - total dissolution has to be achieved within the short residence time in the extruder. Dissolution and dissolution rates are affected by process, material and equipment variables. In this work, we examine the effect of one of the material variables and one of the equipment variables, namely, the API particle size and extruder screw configuration on the API dissolution rate, in a co-rotating, twin-screw extruder. By rapidly removing the extruder screws from the barrel after achieving a steady state, we collected samples along the length of the extruder screws that were characterized by polarized optical microscopy (POM) and differential scanning calorimetry (DSC) to determine the amount of undissolved API. Analyses of samples indicate that reduction of particle size of the API and appropriate selection of screw design can markedly improve the dissolution rate of the API during extrusion. In addition, angle of repose measurements and light microscopy images show that the reduction of particle size of the API can improve the flowability of the physical mixture feed and the adhesiveness between its components, respectively, through dry coating of the polymer particles by the API particles.

  14. Transplante cardíaco ortotópico pela técnica bicaval

    Directory of Open Access Journals (Sweden)

    CHACCUR Paulo

    1997-01-01

    Full Text Available Há trinta anos tem sido empregada a técnica descrita por Lower e Shumway para o transplante cardíaco ortotópico, com bons resultados. Complicações, como estase venosa, formação de trombos, arritmias atriais e insuficiência das valvas tricúspide e mitral, estão presentes no pós-operatório tardio. A partir de 1995, passamos a utilizar a técnica bicaval em todos os casos (6 pacientes. Cinco eram do sexo masculino e a média de idades dos receptores era de 50,6 anos. A cardiomiopatia era dilatada e isquêmica em 2 casos cada, e chagásica e reumática nos demais. Três pacientes apresentavam operações prévias, sendo que um deles havia sido submetido a três operações de prótese valvar aórtica, o segundo a implante de marcapasso epimiocárdico definitivo, e o último a duas operações de revascularização do miocárdio associada a aneurismectomia de ventrículo esquerdo. A técnica cirúrgica consistiu, na maioria dos casos, na anastomose das veias pulmonares, em conjunto com o átrio esquerdo do coração a ser implantado e, a seguir, a da cava superior, inferior, do tronco pulmonar e aorta. Todos os doadores estavam em hospitais da cidade e com retirada múltipla de órgãos. Não tivemos óbito hospitalar e todos os pacientes estão vivos para um período de um a quinze meses de pós-operatório. Não houve diferença significativa quando se comparou as duas técnicas para o tempo de anóxia, tempo de C E C, tempo de implante, presença de taquiarritmias atriais, sangramento e permanência hospitalar. Houve diferença significativa (p<0,05 para a utilização de marcapasso temporário e presença de insuficiência tricúspide. Acreditamos que a técnica bicaval, além de reduzir o tamanho das cavidades atriais, é uma técnica simples, com índice menor de complicações e que poderá ser empregada com mais freqüência.

  15. Transplante cardíaco e infecção

    Directory of Open Access Journals (Sweden)

    COUTO Wilson José

    2001-01-01

    Full Text Available Objetivo: O presente estudo visa avaliar a incidência de infecções, os agentes etiológicos, e a apresentação clínica e a morbi-mortalidade operatória nos pacientes submetidos a transplante cardíaco na Universidade Federal de São Paulo. Casuística e Métodos: No período de novembro de 1996 a junho de 1998, 97 pacientes transplantados pela equipe de Cirurgia Cardiovascular da UNIFESP sobreviveram por período superior a uma semana após o transplante e foram analisados, retrospectivamente, quanto aos processos infecciosos diagnosticados. A idade dos pacientes variou de 3 a 63 anos (média de 44,4 ± 13,0 anos, sendo que a maioria apresentava miocardiopatia dilatada (46, seguida de chagásica (24 e isquêmica (23. O tempo de seguimento variou de 0,33 a 119 meses (36 ± 30,7 meses. Resultados: Dos 97 pacientes estudados, 16 (16,4% morreram, sendo infecção a principal causa, seguido pela rejeição em 10 (10,30%. As causas de óbito por infecção foram: sepse bacteriana em 6 pacientes, pneumonia em outros 6, infecção intra-abdominal em 2, toxoplasmose disseminada em 1 e infecção pelo citomegalovírus em 1. Ocorreram 142 episódios infecciosos diagnosticados distribuídos da seguinte forma: bacterianos 76 (52,5%; virais 34 (28,8%; fúngicos 20 (17,5% e protozoários 12 (12,4%. Houve 8 episódios de reativação da doença de Chagas (média 4,9 meses após o transplante diagnosticados principalmente através do aparecimento de nódulos subcutâneos. Todos os casos de reativação foram tratados com alopurinol e evoluíram bem. Conclusão: Os dados apresentados mostraram o predomínio de infecções bacterianas que causaram maior mortalidade. Nos transplantados por doença de Chagas a reativação da doença pode ser controlada adequadamente com uso de alopurinol. Os dados são importantes para orientação em nossa comunidade dos programas de transplantes, uma vez que traz à tona particularidades do nosso meio.

  16. Étude du comportement des aciers API 5L X60 sollicités par contraintes mécaniques et milieu de sol Algérien Simulé

    OpenAIRE

    AMARA ZENATI, Abdeldjelil

    2014-01-01

    Les essais mécaniques dans le milieu corrosif ont montré que le matériau perd ses caractéristiques mécaniques en contact avec le sol environnant et la résistance mécanique diminue. Une Modélisation des défaillances et estimation de la durée de vie par approche de weibull a été effectuée en passant par le calcul de tous les paramètres et les fonctions de fiabilité afin de définir la durée de vie nominale du pipe et qui a été évaluée entre 10 et 15 ans

  17. Avaliação da resistência à corrosão-fadiga do aço API 5L X60 em ambiente pressurizado contendo CO2

    OpenAIRE

    Marlon Brandi Corrêa

    2010-01-01

    Os risers rígidos, utilizados na indústria offshore para a exploração e transporte de óleo e gás, estão sujeitos a diversus fenômenos de degradação. A fadiga e a corrosãofadiga são uma das causas mais comuns de falhas em serviço destas tubulações. Estas falhas geralmente são encontradas nas regiões submetidas a processos de soldagem. Para uma operação que alie produtividade e segurança os fenômenos de fadiga e corrosão-fadiga devem ser estudados e entendidos a fim de evitá-los ou minimizá-los...

  18. The end of polling: why and how to transform a REST API into a Data Streaming API?

    CERN Document Server

    CERN. Geneva

    2017-01-01

    We know interactivity is the key to keep our user’s interest alive but we can’t reduce animation to UI anymore. Twitter, Waze, Slack… users are used to have real-time data in applications they love. But how can you turn your static API into a stream of data? By pulling? Pushing? Webhook-ing? When talking about data streaming, we often think about WebSockets. But have you ever heard of Server-Sent Events? In this tools-in-action we will compare those technologies to understand which one you should opt for depending on your usecase, and I’ll show you how we have been reducing the amount of data to transfer even further with JSON-Patch. And because real-time data is not only needed by web (and because it’s much more fun), I’ll show you how we can make a drone dance on streamed APIs.

  19. A SNP based high-density linkage map of Apis cerana reveals a high recombination rate similar to Apis mellifera.

    Directory of Open Access Journals (Sweden)

    Yuan Yuan Shi

    Full Text Available BACKGROUND: The Eastern honey bee, Apis cerana Fabricius, is distributed in southern and eastern Asia, from India and China to Korea and Japan and southeast to the Moluccas. This species is also widely kept for honey production besides Apis mellifera. Apis cerana is also a model organism for studying social behavior, caste determination, mating biology, sexual selection, and host-parasite interactions. Few resources are available for molecular research in this species, and a linkage map was never constructed. A linkage map is a prerequisite for quantitative trait loci mapping and for analyzing genome structure. We used the Chinese honey bee, Apis cerana cerana to construct the first linkage map in the Eastern honey bee. RESULTS: F2 workers (N = 103 were genotyped for 126,990 single nucleotide polymorphisms (SNPs. After filtering low quality and those not passing the Mendel test, we obtained 3,000 SNPs, 1,535 of these were informative and used to construct a linkage map. The preliminary map contains 19 linkage groups, we then mapped the 19 linkage groups to 16 chromosomes by comparing the markers to the genome of A. mellfiera. The final map contains 16 linkage groups with a total of 1,535 markers. The total genetic distance is 3,942.7 centimorgans (cM with the largest linkage group (180 loci measuring 574.5 cM. Average marker interval for all markers across the 16 linkage groups is 2.6 cM. CONCLUSION: We constructed a high density linkage map for A. c. cerana with 1,535 markers. Because the map is based on SNP markers, it will enable easier and faster genotyping assays than randomly amplified polymorphic DNA or microsatellite based maps used in A. mellifera.

  20. Aggressiveness index of Apis Mellifera (Hymenoptera: Aapidae) Índice de agresividad en Apis mellifera ( Hymenoptera: Aapidae )

    OpenAIRE

    Sierra Omar Danilo; Insuasty Torres Jennyfer

    2004-01-01

    An index measuring the aggressiveness among ten colonies of Apis mellifera was elaborated based on the third generation synthetic indices by Charum et al. (1999). The index values are subject to a fixed parameter used as the beginning or standard value, and correspond to the aggressive features of some Africans colonies studied by Rothenbuler et al. (1968). In the ten colonies the index values are notably smaller than those of African colonies and are biased to the lowest values. This indicat...

  1. Reporte de un caso de pericarditis hemorrágica y taponamiento cardíaco asociados a lupus eritematoso sistémico

    OpenAIRE

    Barrera-Ramírez,Carlos Felipe; Pineda-Pompa,Luis R; Melo,Mario; Valdez Castro, Ricardo; Medina-Gómez,Héctor; Godina-Alonso,Gustavo; Guzmán,Carlos E

    2005-01-01

    El lupus eritematoso sistémico es una enfermedad autoinmune inflamatoria capaz de afectar cualquier aparato y sistema. Aunque la pericarditis es la manifestación cardíaca más frecuentemente observada, usualmente no es una situación que comprometa la vida del paciente. El taponamiento cardíaco ocasionado por derrame pericárdico en el lupus es una situación extremadamente rara, con una incidencia no mayor al 2%. Informamos el caso de una mujer de 21 años de edad con taponamiento cardíaco por lu...

  2. Efeitos do treinamento físico aeróbio sobre o sistema renina angiotensina cardíaco e sistêmico de ratos obesos

    OpenAIRE

    Diego Lopes Mendes Barretti

    2010-01-01

    Introdução: A obesidade bem como um aumento da ativação do sistema renina angiotensina cardíaco estão profundamente envolvidos com o desenvolvimento de doenças cardiovasculares. Por outro lado, o treinamento físico aeróbio, previne o desenvolvimento da obesidade e reduz o sistema renina angiotensina cardíaco em algumas patologias. Dessa forma, nosso objetivo foi de investigar se a obesidade e sua associação com o treinamento físico aeróbio alteram os componentes do sistema renina angiotensina...

  3. Transient absorption spectroscopy in biology using the Super-ACO storage ring FEL and the synchrotron radiation combination

    CERN Document Server

    Renault, E; De Ninno, G; Garzella, D; Hirsch, M; Nahon, L; Nutarelli, D

    2001-01-01

    The Super-ACO storage ring FEL, covering the UV range down to 300 nm with a high average power (300 mW at 350 nm) together with a high stability and long lifetime, is a unique tool for the performance of users applications. We present here the first pump-probe two color experiments on biological species using a storage ring FEL coupled to the synchrotron radiation. The intense UV pulse of the Super-ACO FEL is used to prepare a high initial concentration of chromophores in their first singlet electronic excited state. The nearby bending magnet synchrotron radiation provides, on the other hand a pulsed, white light continuum (UV-IR), naturally synchronized with the FEL pulses and used to probe the photochemical subsequent events and the associated transient species. We have demonstrated the feasibility with a dye molecule (POPOP) observing a two-color effect, signature of excited state absorption and a temporal signature with Acridine. Applications on various chromophores of biological interest are carried out,...

  4. Manejo de las dislipidemias en pacientes cardíacos trasplantados. Hallazgos sobre nuevos factores de riesgo

    Directory of Open Access Journals (Sweden)

    Walter Masson

    2008-01-01

    Full Text Available En pacientes cardíacos trasplantados, el desarrollo de enfermedad vascular coronaria es una complicación frecuente y la dislipidemia es uno de los predictores más importantes. Los inmunosupresores predisponen a las dislipidemias y dificultan la utilización de hipolipemiantes. En este grupo particular de pacientes se recomienda alcanzar las metas terapéuticas de prevención secundaria. Las estatinas son los hipolipemiantes de elección. No existen recomendaciones claras sobre nuevos factores de riesgo, como la homocisteína y la lipoproteína (a [Lp(a].Con el objetivo de conocer el perfil lipídico, la prevalencia de homocisteinemia y de Lp(a elevadas, el cumplimiento de las metas terapéuticas y la tolerancia a la medicación, se incluyeron en el estudio 23 pacientes cardíacos trasplantados. Los resultados mostraron que el cumplimiento de las metas lipídicas fue aceptable y que el 65% recibía tratamiento hipolipemiante. El uso de estatinas fue seguro. Se encontró una prevalencia alta de homocisteína y Lp(a elevadas. Su implicación en la modificación del tratamiento se desconoce.

  5. Interacciones entre dispositivos cardíacos implantables y modalidades fisioterapéuticas: ¿Mito o realidad?

    Directory of Open Access Journals (Sweden)

    Genevieve C. Digby

    2011-04-01

    Full Text Available La fisioterapia se ha transformado en una especialidad que claramente incide en la calidad de vida de nuestros pacientes. En poblaciones añosas, el uso de fisioterapia incluye múltiples modalidades para un alto número de distintas enfermedades. Varios informes sobre posibles interacciones negativas entre las distintas modalidades de fisioterapia y los dispositivos cardíacos implantables (marcapasos y cardiodesfibriladores han sido publicados en los últimos aňos. A pesar de ello, existe muy poca evidencia y guías precisas para identificar cuáles son las modalidades de fisioterapia seguras a utilizar en esta población de pacientes. En la siguiente revisión, nos propusimos resumir las interacciones documentadas entre fisioterapia y dispositivos cardíacos implantables (DCI, discutir el estándar actual de estas prácticas e identificar las principales consideraciones que existen desde la perspectiva de un servicio de electrofisiología cardíaca, para el tratamiento adecuado en estos pacientes. Finalmente, abogamos por fortalecer la colaboración entre fisioterapeutas y electrofisiólogos, con el fin de asegurar una óptima y segura atención de este grupo de pacientes.

  6. Is the Sample Good Enough? Comparing Data from Twitter's Streaming API with Twitter's Firehose

    OpenAIRE

    Morstatter, Fred; Pfeffer, Jürgen; Liu, Huan; Carley, Kathleen M.

    2013-01-01

    Twitter is a social media giant famous for the exchange of short, 140-character messages called "tweets". In the scientific community, the microblogging site is known for openness in sharing its data. It provides a glance into its millions of users and billions of tweets through a "Streaming API" which provides a sample of all tweets matching some parameters preset by the API user. The API service has been used by many researchers, companies, and governmental institutions that want to extract...

  7. Use of the API 20E system to identify veterinary Enterobacteriaceae.

    OpenAIRE

    Swanson, E C; Collins, M T

    1980-01-01

    A total of 503 veterinary enteric bacterial pathogens obtained from state veterinary diagnostic laboratories were tested on API 20E strips to determine whether this rapid microidentification system could be utilized for veterinary clinical microbiology. The API 20E strip accurately identified 96% of the veterinary isolates and misidentified 3%. Identifications by the API system and the diagnostic laboratories were in agreement in 85% of the isolates, disagreement on 16% of the isolates, and 1...

  8. Imunocitomonitorização em pacientes transplantados cardíacos Immunocytomonitorization in patients submitted to cardiac transplantation

    Directory of Open Access Journals (Sweden)

    Ricardo Manrique

    1990-04-01

    Full Text Available A técnica de Imunocitomonitorização (CIM - Cytologrcal and Immunologic Monitoring, fundamenta-se na avaliação de mudanças nas subpopulações de linfócitos e seus precursores celulares, durante o processo rejeição aguda. O método avalia a ativação precoce pela contagem do número de: linfócitos ativados, linfoblastos e prolinfócitos (aqui chamados em conjunto de linfócitos ativos, quando esses se apresentam acima do limite superior normal. Utilizamos essa técnica para seguimento e controle da atividade da imunologia celular em 9 pacientes transplantados cardíacos. Também foram submetidos ao CIM para verificar a faixa de normalidade de ativação linfocítica 20 voluntários normais e cinco pacientes candidatos ao transplante cardíaco. Ambos os grupos não tinham antecedentes de quadro de infecção ou alteração imunológica. Para obtenção do controle positivo, testamos 10 pacientes com infecção bacteriana ou virai, previamente diagnosticados por exames bacterianos e/ou sorológicos. Foram observados valores de 1,8% de linfócitos ativos nos indivíduos normais e 3,2% nos candidatos ao transplante cardíaco. Dos 63 testes realizados nos transplantados cardíacos, quatro deles se mostraram positivos, onde três desses tratavam-se de casos de infecção e um confirmado como processo de rejeição aguda em evolução. O tempo médio necessário para a realização da CIM foi de aproximadamente duas horas. A técnica utiliza mostrou-se valiosa no reconhecimento imunológico dos pacientes transplantados cardíacos, pela sua sensibilidade diagnostica, baixo custo e rapidez.The immunocytomonitorization technique (CIM - Cytological and Immunologic Monitoring is based on assessment of changes in the subpopulations of lyphocyte and their precursor cells, while under the acute rejection. The method assesses the precocious activation by counting the number of the lymphocytes normal and actives, lymphoblasts and prolymphocytes (here

  9. Intelligent Search Method Based ACO Techniques for a Multistage Decision Problem EDP/LFP

    Directory of Open Access Journals (Sweden)

    Mostefa RAHLI

    2006-07-01

    the algorithm getting to him a rate preferably more or less justifiable. In operational research, this subject is known under the name of CPO [14] (combinatory problem optimization.The choice of a numerical method to use for a merged case study and calculation of the LFP/Fitting/EDP what is [7, 8, 9, 10, 18, 19, 20] (in theoretical form of a problem compensates the final decision to adopt and a strategy of optimal production (which is a practical problem form and the final task most wanted.Each method is imposed by:· The algorithm complexity.· In an application gathering all calculations, the number of uses of method compared to the total number of later issues.· The maximum number of iterations for a given use.· The maximum iterations count allowed for this algorithm kind.· The limitations of the algorithm such as: applicability of a method (algorithm adapted or not to the problem; does the problem constrained or not; problem dimension or order N (N ≤ Nmax; the algorithm stability.It's well-known that for an approached calculation method, the propagation of errors strongly conditions the need of making its adequate choice and if it can be adopted compared to others for the same area.More is the number of the elementary operations is large more the final result misses precision and especially if the finality of the study is a responsible decision to make and a satisfaction of constraints and multiple conditions. Our study proposes an inference based solution (AI with the use of ACO technique (Ant colony Optimization2.

  10. Experiencia con hipotermia terapéutica en el paro cardíaco extrahospitalario

    Directory of Open Access Journals (Sweden)

    Ricardo Levin

    2016-01-01

    Full Text Available Introducción: El paro cardíaco extrahospitalario (PCEH, una de las principales causas de mortalidad en el mundo occidental, se asocia con una muy baja sobrevida hospitalaria y con un número elevado de secuelas neurológicas entre los sobrevivientes, sin modificaciones significativas en el pronóstico en las últimas tres décadas. Estudios clínicos más recientes plantearon la utilidad de la hipotermia terapéutica en la reducción de la mortalidad y el grado de deterioro neurológico en sobrevivientes de un PCEH. Objetivos: Evaluar la mortalidad y el resultado neurológico de pacientes comatosos resucitados de un PCEH tratados con hipotermia terapéutica. Considerar las indicaciones y los hallazgos de la cinecoronariografía y detectar complicaciones asociadas con la hipotermia. Material y métodos: Se incluyeron pacientes resucitados de un PCEH tratados bajo hipotermia terapéutica, que consistió en una fase de enfriamiento a 33 °C, un mantenimiento durante 24 horas y recalentamiento a 0,25 °C/hora. Se analizaron las indicaciones y los hallazgos de la cinecoronariografía. Se evaluaron complicaciones metabólicas, arrítmicas, infecciosas y hemorrágicas. Se consideró significativo valor de p menor de 0,05. Resultados: De los 213 pacientes incluidos, la sobrevida hospitalaria fue de 109 pacientes (51,2%, de los que 96 (46,1% presentaron al egreso buena recuperación neurológica. Se observó mayor sobrevida en pacientes con ritmos pasibles de desfibrilación respecto de aquellos con ritmos no pasibles de desfibrilación (58,2% vs. 37,7%; p = 0,007. Se derivaron (3,5 horas en promedio a cinecoronariografía 147 (69% pacientes, de los cuales 121 (82,3% mostraron enfermedad significativa, observándose en 69 (57% oclusión coronaria completa. Ciento siete pacientes fueron sometidos a angioplastia; 43 (40,2% de ellos fallecieron durante la internación frente a 61 (58,6% óbitos entre los no intervenidos o no estudiados (p = 0,005. Entre

  11. Expression Study of LeGAPDH, LeACO1, LeACS1A, and LeACS2 in Tomato Fruit (Solanum lycopersicum

    Directory of Open Access Journals (Sweden)

    Pijar Riza Anugerah

    2015-10-01

    Full Text Available Tomato is a climacteric fruit, which is characterized by ripening-related increase of respiration and elevated ethylene synthesis. Ethylene is the key hormone in ripening process of climacteric fruits. The objective of this research is to study the expression of three ethylene synthesis genes: LeACO1, LeACS1A, LeACS2, and a housekeeping gene LeGAPDH in ripening tomato fruit. Specific primers have been designed to amplify complementary DNA fragment of LeGAPDH (143 bp, LeACO1 (240 bp, LeACS1A (169 bp, and LeACS2 (148 bp using polymerase chain reaction. Nucleotide BLAST results of the complementary DNA fragments show high similarity with LeGAPDH (NM_001247874.1, LeACO1 (NM_001247095.1, LeACS1A(NM_001246993.1, LeACS2 (NM_001247249.1, respectively. Expression study showed that LeACO1, LeACS1A, LeACS2, and LeGAPDH genes were expressed in ripening tomato fruit. Isolation methods, reference sequences, and primers used in this study can be used in future experiments to study expression of genes responsible for ethylene synthesis using quantitative polymerase chain reaction and to design better strategy for controlling fruit ripening in agroindustry.

  12. Solar Eclipse Computer API: Planning Ahead for August 2017

    Science.gov (United States)

    Bartlett, Jennifer L.; Chizek Frouard, Malynda; Lesniak, Michael V.; Bell, Steve

    2016-01-01

    With the total solar eclipse of 2017 August 21 over the continental United States approaching, the U.S. Naval Observatory (USNO) on-line Solar Eclipse Computer can now be accessed via an application programming interface (API). This flexible interface returns local circumstances for any solar eclipse in JavaScript Object Notation (JSON) that can be incorporated into third-party Web sites or applications. For a given year, it can also return a list of solar eclipses that can be used to build a more specific request for local circumstances. Over the course of a particular eclipse as viewed from a specific site, several events may be visible: the beginning and ending of the eclipse (first and fourth contacts), the beginning and ending of totality (second and third contacts), the moment of maximum eclipse, sunrise, or sunset. For each of these events, the USNO Solar Eclipse Computer reports the time, Sun's altitude and azimuth, and the event's position and vertex angles. The computer also reports the duration of the total phase, the duration of the eclipse, the magnitude of the eclipse, and the percent of the Sun obscured for a particular eclipse site. On-line documentation for using the API-enabled Solar Eclipse Computer, including sample calls, is available (http://aa.usno.navy.mil/data/docs/api.php). The same Web page also describes how to reach the Complete Sun and Moon Data for One Day, Phases of the Moon, Day and Night Across the Earth, and Apparent Disk of a Solar System Object services using API calls.For those who prefer using a traditional data input form, local circumstances can still be requested that way at http://aa.usno.navy.mil/data/docs/SolarEclipses.php. In addition, the 2017 August 21 Solar Eclipse Resource page (http://aa.usno.navy.mil/data/docs/Eclipse2017.php) consolidates all of the USNO resources for this event, including a Google Map view of the eclipse track designed by Her Majesty's Nautical Almanac Office (HMNAO). Looking further ahead, a

  13. Ford's new 3.5 l V6 gasoline engine. Downsizing with 'EcoBoost'; Der neue 3,5-l-V6-Ottomotor von Ford. Downsizing durch 'EcoBoost'

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, Gerhard; Kapp, Daniel; Schamel, Andreas; Hinds, Brett; Weaver, Corey [Ford Motor Company, Dearborn, MI (United States)

    2009-03-15

    Ford banded a new motor technology as 'EcoBoost'. It applies direct fuel injection and turbocharging to gasoline engines and provides a major improvement in fuel economy via engine downsizing, combined with improved performance. The first Ford engine with this technology is the 3.5 l V6 EcoBoost in the 2010 Lincoln MKS luxury sedan. Combustion, fuel, boost, and power conversion systems are discussed in detail with emphasis on the efficiency and value of these systems. Engine level fuel economy and performance are discussed, and compared to conventional gasoline engines. (orig.)

  14. Uma breve revisão histórica do desenvolvimento da soldagem dos aços API para tubulações A brief history review of development on API steels welding for pipeline

    Directory of Open Access Journals (Sweden)

    Jaime Casanova Soeiro Junior

    2013-06-01

    Full Text Available Este trabalho enfoca o desenvolvimento dos aços API para tubulações desde o aço API 5L X42, desenvolvido em 1948, através de laminação a quente seguida de tratamento térmico. Desde então foram feitos diferentes desenvolvimentos visando aumentar a resistência mecânica e a tenacidade, além de melhorar a soldabilidade. Entre eles, a adição de elementos de liga, produzindo os aços ARBL e a utilização de rotas de processamento termomecânico durante a laminação. Inicialmente a rota utilizada era a laminação a quente controlada (TMCR, com utilização de elemento microligante como Nb para os aços X60 e o conjunto Nb e V para os aços X65 e X70, associado a uma redução do teor de carbono. Posteriormente foi implantada a rota de fabricação do aço por laminação controlada seguida de resfriamento controlado (TMCP, com redução de carbono e adição de Nb e Ti para os aços X80, e adições de Mo, Nb, Be Ti para os X100. A redução do carbono foi motivada também para melhorar a sua soldabilidade e, por este motivo, estes aços são soldados por diferentes processos de soldagem, como o eletrodo revestido. Desenvolvimentos recentes com raiz feita com MAG-TC e enchimento com arame tubular tem dado resultados animadores.This paper is a brief history on the development of pipelines steels since the API 5L X42 steel, developed in 1948 by hot rolling followed by heat treatment. Subsequently different developments were made to increase the strength and toughness of these materials, and to improve their weldability. Among them, the addition of microalloying elements, producing HSLA steels and thermomechanical processing routes during hot rolling. Initially the route used was the controlled hot rolling (TMCR, together with microalloying element such as Nb for X60 steel and Nb plus V for X65 and X70 steels, associated with a reduction in carbon content. Later, the thermomechanical controlled process (TMCP manufacturing route was

  15. Certeza diagnóstica en la mortalidad de una población de pacientes con trasplante cardíaco

    Directory of Open Access Journals (Sweden)

    Marcos Amuchástegui

    2008-01-01

    Full Text Available RESUMENIntroducciónA pesar de que la morbimortalidad en el trasplante cardíaco ha sido motivo de extensoanálisis, la mayoría de los estudios y registros de mortalidad en pacientes trasplantados sebasan sobre datos clínicos. En la bibliografía existen comunicaciones aisladas de autopsiasen pacientes con trasplante cardíaco.ObjetivoDeterminar la importancia de la realización de estudios anatomopatológicos para el diagnósticode causa de muerte en un programa de trasplante cardíaco.Material y métodosSe incluyeron todos los pacientes con trasplante cardíaco fallecidos entre enero 1990 y enero2005. El diagnóstico definitivo de la causa de muerte fue corroborado por autopsia obiopsia de órgano sólido. Las causas de muerte evaluadas fueron falla precoz del injerto,rechazo celular, infección, enfermedad vascular del injerto, neoplasia y otros.ResultadosDurante el período en estudio 73 pacientes fueron sometidos a trasplante cardíaco; de ellos,fallecieron 31. Se obtuvieron 12 autopsias y 7 biopsias de órgano sólido que certificaron lacausa de muerte (61%. La causa de muerte más frecuente fue el rechazo celular mayor degrado III. En el 12,9%, la anatomía patológica difirió de la sospecha clínica de la causa demuerte.ConclusiónLa información clinicopatológica derivada de estudios post mortem es un indicador de nuestrarealidad asistencial y se constituye en un pilar fundamental para el conocimiento y elmanejo futuro de los pacientes trasplantados, por lo que consideramos que la realización deautopsias en estos pacientes es de vital importancia.REV ARGENT CARDIOL 2008;76:292-294.

  16. Certeza diagnóstica en la mortalidad de una población de pacientes con trasplante cardíaco

    Directory of Open Access Journals (Sweden)

    Marcos Amuchástegui (h,

    2006-01-01

    Full Text Available Introducción A pesar de que la morbimortalidad en el trasplante cardíaco ha sido motivo de extenso análisis, la mayoría de los estudios y registros de mortalidad en pacientes trasplantados se basan sobre datos clínicos. En la bibliografía existen comunicaciones aisladas de autopsias en pacientes con trasplante cardíaco.Objetivo Determinar la importancia de la realización de estudios anatomopatológicos para el diagnóstico de causa de muerte en un programa de trasplante cardíaco.Material y métodosSe incluyeron todos los pacientes con trasplante cardíaco fallecidos entre enero 1990 y enero 2005. El diagnóstico definitivo de la causa de muerte fue corroborado por autopsia o biopsia de órgano sólido. Las causas de muerte evaluadas fueron falla precoz del injerto, rechazo celular, infección, enfermedad vascular del injerto, neoplasia y otros.ResultadosDurante el período en estudio 73 pacientes fueron sometidos a trasplante cardíaco; de ellos, fallecieron 31. Se obtuvieron 12 autopsias y 7 biopsias de órgano sólido que certificaron la causa de muerte (61%. La causa de muerte más frecuente fue el rechazo celular mayor de grado III. En el 12,9%, la anatomía patológica difirió de la sospecha clínica de la causa de muerte.ConclusiónLa información clinicopatológica derivada de estudios post mortem es un indicador de nuestra realidad asistencial y se constituye en un pilar fundamental para el conocimiento y el manejo futuro de los pacientes trasplantados, por lo que consideramos que la realización de autopsias en estos pacientes es de vital importancia.

  17. Macelignan inhibits bee pathogenic fungi Ascophaera apis growth through HOG1 pathway

    Directory of Open Access Journals (Sweden)

    Y.K. Shin

    2016-01-01

    Full Text Available Ascosphaera apis is a bee pathogen that causes bee larvae infection disease, to which treatment is not yet well investigated. The aim of this study was to investigate antifungal susceptibility in vitro against A. apis and to identify a new antifungal agent for this pathogen through minimal inhibitory concentration (MIC assay and western blot analysis. Macelignan had 1.56 and 3.125 μg/mL MIC against A. apis after 24 and 48 h, respectively, exhibiting the strongest growth inhibition against A. apis among the tested compounds (corosolic acid, dehydrocostus lactone, loganic acid, tracheloside, fangchinoline and emodin-8-O-β-D-glucopyranoside. Furthermore, macelignan showed a narrow-ranged spectrum against various fungal strains without any mammalian cell cytotoxicity. In spite of miconazole having powerful broad-ranged anti-fungal activity including A. apis, it demonstrated strong cytotoxicity. Therefore, even if macelignan alone was effective as an antifungal agent to treat A. apis, combined treatment with miconazole was more useful to overcome toxicity, drug resistance occurrence and cost effectiveness. Finally, HOG1 was revealed as a target molecule of macelignan in the anti-A. apis activity by inhibiting phosphorylation using S. cerevisiae as a model system. Based on our results, macelignan, a food-grade antimicrobial compound, would be an effective antifungal agent against A. apis infection in bees.

  18. Improvements and extensions to API 618 related to pulsation and mechanical response studies

    NARCIS (Netherlands)

    Eijk, A.; Smeulers, J.P.M.; Blodgett, L.E.; Smalley, A.J.

    1999-01-01

    Purchasers of reciprocating compressors need a standard to help ensure reliable installations. The PNEUROP standard evolved in Europe, and the API 618 Standard in the U.S.A. Frequent application and generally good experience, worldwide, have led to four releases of API 618, the last dated June 1995.

  19. Influence of humidity on the phase behavior of API/polymer formulations.

    Science.gov (United States)

    Prudic, Anke; Ji, Yuanhui; Luebbert, Christian; Sadowski, Gabriele

    2015-08-01

    Amorphous formulations of APIs in polymers tend to absorb water from the atmosphere. This absorption of water can induce API recrystallization, leading to reduced long-term stability during storage. In this work, the phase behavior of different formulations was investigated as a function of relative humidity. Indomethacin and naproxen were chosen as model APIs and poly(vinyl pyrrolidone) (PVP) and poly(vinyl pyrrolidone-co-vinyl acetate) (PVPVA64) as excipients. The formulations were prepared by spray drying. The water sorption in pure polymers and in formulations was measured at 25°C and at different values of relative humidity (RH=25%, 50% and 75%). Most water was absorbed in PVP-containing systems, and water sorption was decreasing with increasing API content. These trends could also be predicted in good agreement with the experimental data using the thermodynamic model PC-SAFT. Furthermore, the effect of absorbed water on API solubility in the polymer and on the glass-transition temperature of the formulations was predicted with PC-SAFT and the Gordon-Taylor equation, respectively. The absorbed water was found to significantly decrease the API solubility in the polymer as well as the glass-transition temperature of the formulation. Based on a quantitative modeling of the API/polymer phase diagrams as a function of relative humidity, appropriate API/polymer compositions can now be selected to ensure long-term stable amorphous formulations at given storage conditions.

  20. Emission “Off-On” effect from europium complexes triggered by AcO anion: Synthesis, characterization and sensing performance

    Science.gov (United States)

    Li, Xiaogang; Zhang, Dong; Li, Jing

    2014-06-01

    In this paper, a series of Eu(III) complexes based on four diamine ligands and two diketone ligands were synthesized. Their single crystal structures were studied, where intermolecular π-π stacking was found. The photophysical parameters of these Eu(III) complexes were measured, along with their ligand triplet levels. The energy transfer mechanism between ligand and metal center was discussed in detail. Energy transfer roll-back was found in Eu(III) complexes owing large-conjugated diamine ligands, compromising emissive performance. This energy transfer roll-back, however, could be stopped by the presence of AcO anion, leading to Eu(III) complex emission enhancement. The sensing performance of such Eu(III) complexes was thus investigated in detail. High sensitivity and selectivity were observed.

  1. Emission "Off-On" effect from europium complexes triggered by AcO anion: synthesis, characterization and sensing performance.

    Science.gov (United States)

    Li, Xiaogang; Zhang, Dong; Li, Jing

    2014-06-05

    In this paper, a series of Eu(III) complexes based on four diamine ligands and two diketone ligands were synthesized. Their single crystal structures were studied, where intermolecular π-π stacking was found. The photophysical parameters of these Eu(III) complexes were measured, along with their ligand triplet levels. The energy transfer mechanism between ligand and metal center was discussed in detail. Energy transfer roll-back was found in Eu(III) complexes owing large-conjugated diamine ligands, compromising emissive performance. This energy transfer roll-back, however, could be stopped by the presence of AcO anion, leading to Eu(III) complex emission enhancement. The sensing performance of such Eu(III) complexes was thus investigated in detail. High sensitivity and selectivity were observed.

  2. Segmentación de Sonidos Cardíacos mediante Procesado Digital de Señal

    OpenAIRE

    Ezennaya Gómez, Salatiel

    2012-01-01

    Una de las técnicas más utilizadas en medicina para la detección o sospecha de una enfermedad cardíaca es la auscultación. Es importante mejorar ésta técnica para dar un apoyo tecnológico y de procesado en la interpretación al especialista. Durante las últimas décadas se han realizado numerosos estudios para la mejora de la detección de sonidos cardíacos procedentes de fonocardiógrafos y estetoscopios digitales. Se plantean diferentes métodos de procesado para la mejora de la segmentación...

  3. APIS - a novel approach for conditioning honey bees

    Directory of Open Access Journals (Sweden)

    Nicholas Hagen Kirkerud

    2013-04-01

    Full Text Available Honey bees perform robustly in different conditioning paradigms. This makes them excellent candidates for studying mechanisms of learning and memory at both an individual and a population level. Here we introduce a novel method of honey bee conditioning: APIS, the Automatic Performance Index System. In an enclosed walking arena where the interior is covered with an electric grid, presentation of odours from either end can be combined with weak electric shocks to form aversive associations. To quantify behavioural responses, we continuously monitor the movement of the bee by an automatic tracking system. We found that escapes from one side to the other, changes in velocity as well as distance and time spent away from the punished odour are suitable parameters to describe the bee’s learning capabilities.Our data show that in a short-term memory test the response rate for the conditioned stimulus in APIS correlates well with response rate obtained from conventional Proboscis Extension Response (PER-conditioning. Additionally, we discovered that bees modulate their behaviour to aversively learned odours by reducing their rate, speed and magnitude of escapes and that both generalisation and extinction seem to be different between appetitive and aversive stimuli. The advantages of this automatic system make it ideal for assessing learning rates in a standardised and convenient way, and its flexibility adds to our toolbox for studying honey bee behaviour.

  4. From where did the Western honeybee (Apis mellifera) originate?

    Science.gov (United States)

    Han, Fan; Wallberg, Andreas; Webster, Matthew T

    2012-08-01

    The native range of the honeybee Apis mellifera encompasses Europe, Africa, and the Middle East, whereas the nine other species of Apis are found exclusively in Asia. It is therefore commonly assumed that A. mellifera arose in Asia and expanded into Europe and Africa. However, other hypotheses for the origin of A. mellifera have also been proposed based on phylogenetic trees constructed from genetic markers. In particular, an analysis based on >1000 single-nucleotide polymorphism markers placed the root of the tree of A. mellifera subspecies among samples from Africa, suggestive of an out-of-Africa expansion. Here, we re-evaluate the evidence for this and other hypotheses by testing the robustness of the tree topology to different tree-building methods and by removing specimens with a potentially hybrid background. These analyses do not unequivocally place the root of the tree of A. mellifera subspecies within Africa, and are potentially consistent with a variety of hypotheses for honeybee evolution, including an expansion out of Asia. Our analyses also support high divergence between western and eastern European populations of A. mellifera, suggesting they are likely derived from two distinct colonization routes, although the sources of these expansions are still unclear.

  5. Genetic variation in natural honeybee populations, Apis mellifera capensis

    Science.gov (United States)

    Hepburn, Randall; Neumann, Peter; Radloff, Sarah E.

    2004-09-01

    Genetic variation in honeybee, Apis mellifera, populations can be considerably influenced by breeding and commercial introductions, especially in areas with abundant beekeeping. However, in southern Africa apiculture is based on the capture of wild swarms, and queen rearing is virtually absent. Moreover, the introduction of European subspecies constantly failed in the Cape region. We therefore hypothesize a low human impact on genetic variation in populations of Cape honeybees, Apis mellifera capensis. A novel solution to studying genetic variation in honeybee populations based on thelytokous worker reproduction is applied to test this hypothesis. Environmental effects on metrical morphological characters of the phenotype are separated to obtain a genetic residual component. The genetic residuals are then re-calculated as coefficients of genetic variation. Characters measured included hair length on the abdomen, width and length of wax plate, and three wing angles. The data show for the first time that genetic variation in Cape honeybee populations is independent of beekeeping density and probably reflects naturally occurring processes such as gene flow due to topographic and climatic variation on a microscale.

  6. Parasitic Cape honeybee workers, Apis mellifera capensis, evade policing

    Science.gov (United States)

    Martin, Stephen J.; Beekman, Madeleine; Wossler, Theresa C.; Ratnieks, Francis L. W.

    2002-01-01

    Relocation of the Cape honeybee, Apis mellifera capensis, by bee-keepers from southern to northern South Africa in 1990 has caused widespread death of managed African honeybee, A. m. scutellata, colonies. Apis mellifera capensis worker bees are able to lay diploid, female eggs without mating by means of automictic thelytoky (meiosis followed by fusion of two meiotic products to restore egg diploidy), whereas workers of other honeybee subspecies are able to lay only haploid, male eggs. The A. m. capensis workers, which are parasitizing and killing A. m. scutellata colonies in northern South Africa, are the asexual offspring of a single, original worker in which the small amount of genetic variation observed is due to crossing over during meiosis (P. Kryger, personal communication). Here we elucidate two principal mechanisms underlying this parasitism. Parasitic A. m. capensis workers activate their ovaries in host colonies that have a queen present (queenright colonies), and they lay eggs that evade being killed by other workers (worker policing)-the normal fate of worker-laid eggs in colonies with a queen. This unique parasitism by workers is an instance in which a society is unable to control the selfish actions of its members.

  7. Polymorphism analysis of csd gene in six Apis mellifera subspecies.

    Science.gov (United States)

    Wang, Zilong; Liu, Zhiyong; Wu, Xiaobo; Yan, Weiyu; Zeng, Zhijiang

    2012-03-01

    The complementary sex determination (csd) gene is the primary gene determining the gender of honey bees (Apis spp). In this study we analyzed the polymorphism of csd gene in six Apis mellifera subspecies. The genomic region 3 of csd gene in these six A. mellifera was cloned, and identified. A total of 79 haplotypes were obtained from these six subspecies. Analysis showed that region 3 of csd gene has a high level of polymorphism in all the six A. mellifera subspecies. The A. m. anatolica subspecies has a slightly higher nucleotide diversity (π) than other subspecies, while the π values showed no significant difference among the other five subspecies. The phylogenetic tree showed that all the csd haplotypes from different A. mellifera subspecies are scattered throughout the tree, without forming six different clades. Population differentiation analysis showed that there are significant genetic differentiations among some of the subspecies. The NJ phylogenetic tree showed that the A. m. caucasica and A. m. carnica have the closest relationship, followed by A. m. ssp, A. m. ligustica, A. m. carpatica and A. m. anatolica that were gathered in the tree in turn.

  8. 往复压缩机标准API-618、API-11P的异同点及其应用浅谈%The Similarities and Differences of Reciprocating Compressor Standards of API-618 and API-11P and Discussion of Its Application

    Institute of Scientific and Technical Information of China (English)

    李明悦; 扈滨

    2011-01-01

    PI Standard 618 and API Specification 11P are the standards for reciprocating compressors by American Petroleum Institute ( API) .This paper has speeified the applications of the two standards in the compressors which used in the Cas Treatment Plant of SES natural gas pmject.And the similarities and differences between them are compared.%API Standard 618和API Specification 11P都是美国石油学会(API)针对往复压缩机制定的标准.结合中海石油SES天然气项目中的气体处理厂压缩机单元对这两个标准的应用,对两者之间的异同点进行比较.

  9. Towards Standardized Patient Data Exchange: Integrating a FHIR Based API for the Open Medical Record System.

    Science.gov (United States)

    Kasthurirathne, Suranga N; Mamlin, Burke; Grieve, Grahame; Biondich, Paul

    2015-01-01

    Interoperability is essential to address limitations caused by the ad hoc implementation of clinical information systems and the distributed nature of modern medical care. The HL7 V2 and V3 standards have played a significant role in ensuring interoperability for healthcare. FHIR is a next generation standard created to address fundamental limitations in HL7 V2 and V3. FHIR is particularly relevant to OpenMRS, an Open Source Medical Record System widely used across emerging economies. FHIR has the potential to allow OpenMRS to move away from a bespoke, application specific API to a standards based API. We describe efforts to design and implement a FHIR based API for the OpenMRS platform. Lessons learned from this effort were used to define long term plans to transition from the legacy OpenMRS API to a FHIR based API that greatly reduces the learning curve for developers and helps enhance adhernce to standards.

  10. The Ruby UCSC API: accessing the UCSC genome database using Ruby

    Directory of Open Access Journals (Sweden)

    Mishima Hiroyuki

    2012-09-01

    Full Text Available Abstract Background The University of California, Santa Cruz (UCSC genome database is among the most used sources of genomic annotation in human and other organisms. The database offers an excellent web-based graphical user interface (the UCSC genome browser and several means for programmatic queries. A simple application programming interface (API in a scripting language aimed at the biologist was however not yet available. Here, we present the Ruby UCSC API, a library to access the UCSC genome database using Ruby. Results The API is designed as a BioRuby plug-in and built on the ActiveRecord 3 framework for the object-relational mapping, making writing SQL statements unnecessary. The current version of the API supports databases of all organisms in the UCSC genome database including human, mammals, vertebrates, deuterostomes, insects, nematodes, and yeast. The API uses the bin index—if available—when querying for genomic intervals. The API also supports genomic sequence queries using locally downloaded *.2bit files that are not stored in the official MySQL database. The API is implemented in pure Ruby and is therefore available in different environments and with different Ruby interpreters (including JRuby. Conclusions Assisted by the straightforward object-oriented design of Ruby and ActiveRecord, the Ruby UCSC API will facilitate biologists to query the UCSC genome database programmatically. The API is available through the RubyGem system. Source code and documentation are available at https://github.com/misshie/bioruby-ucsc-api/ under the Ruby license. Feedback and help is provided via the website at http://rubyucscapi.userecho.com/.

  11. EQ-5D-5L in the General German Population: Comparison and Evaluation of Three Yearly Cross-Section Surveys.

    Science.gov (United States)

    Huber, Manuel B; Reitmeir, Peter; Vogelmann, Martin; Leidl, Reiner

    2016-03-21

    Health-related quality of life (HRQoL) is a key measure for evaluating health status in populations. Using the recent EQ-5D-5L for measurement, this study analyzed quality of life results and their stability over consecutive population surveys. Three cross-section surveys for representative samples of the general German population from 2012, 2013, and 2014 were evaluated using the EQ-5D-5L descriptive system and valuation by the Visual Analog Scale (VAS). Aggregated sample size reached 6074. The dimension with the highest prevalence of problems was pain/discomfort (31.7%). Compared with 2012 (59.3%), the percentage of participants in the best health state increased slightly in 2013 (63.4%) and 2014 (62%). Over the 3-year period, diabetes and heart disease had the strongest negative influence on mean VAS result. The number of reported chronic diseases cumulatively reduced mean VAS. Extreme problems in one or more dimensions were stated by only 0.1%-0.2% of patients. Of the potential 247 health states with a problem score ≥ 20, only six were observed in the aggregated sample. HRQoL results were fairly stable over the 3 years, but the share of the population with no problems was not. Results from the aggregated sample may serve as updated reference values for the general German population.

  12. EQ-5D-5L in the General German Population: Comparison and Evaluation of Three Yearly Cross-Section Surveys

    Directory of Open Access Journals (Sweden)

    Manuel B. Huber

    2016-03-01

    Full Text Available Health-related quality of life (HRQoL is a key measure for evaluating health status in populations. Using the recent EQ-5D-5L for measurement, this study analyzed quality of life results and their stability over consecutive population surveys. Three cross-section surveys for representative samples of the general German population from 2012, 2013, and 2014 were evaluated using the EQ-5D-5L descriptive system and valuation by the Visual Analog Scale (VAS. Aggregated sample size reached 6074. The dimension with the highest prevalence of problems was pain/discomfort (31.7%. Compared with 2012 (59.3%, the percentage of participants in the best health state increased slightly in 2013 (63.4% and 2014 (62%. Over the 3-year period, diabetes and heart disease had the strongest negative influence on mean VAS result. The number of reported chronic diseases cumulatively reduced mean VAS. Extreme problems in one or more dimensions were stated by only 0.1%–0.2% of patients. Of the potential 247 health states with a problem score ≥20, only six were observed in the aggregated sample. HRQoL results were fairly stable over the 3 years, but the share of the population with no problems was not. Results from the aggregated sample may serve as updated reference values for the general German population.

  13. Experiência com transplante cardíaco heterotópico em pacientes com resistência pulmonar elevada: seguimento tardio Experiencia con trasplante cardíaco heterotópico en pacientes con resistencia pulmonar elevada: seguimiento tardío Experience with heterotopic heart transplantation in patients with elevated pulmonary vascular resistance: late follow-up

    OpenAIRE

    Jose Henrique Andrade Vila; José Pedro da Silva; Luciana da Fonseca; José Francisco Baumgratz; Américo Tangari Jr; Weverton Ferreira Leite; Claudia Jesus Guilhen; Egas Armelin

    2010-01-01

    FUNDAMENTO: Nos últimos anos o numero de artigos sobre transplante cardíaco heterotópico tem sido escasso na literatura, inclusive internacional, e em particular do seguimento de longo prazo destes pacientes, o que levou ao presente relato. OBJETIVO: Relatar a experiência clínica inicial e evolução tardia de quatro pacientes submetidos a transplante cardíaco heterotópico, sua indicação e principais complicações. MÉTODOS: As cirurgias ocorreram entre 1992 e 2001, sendo que a indicação de trans...

  14. Asynchronous API Pattern and its Application%异步接口模式及其应用

    Institute of Scientific and Technical Information of China (English)

    何华海; 丁柯

    2002-01-01

    In distributed systems,high efficiency can be achieved using asynchronous API between client and server.This paper provides an architectural pattern that implements asynchronous API generally. Asynchronous methods donot execute operations directly,however,they delegate the sending and receiving process to individual threads via aqueue ,the client deals with results by means of callback ,wait or check. Synchronous API is implemented on the baseof asynchronous API. Presently the asynchronous API pattern has been employed in the implementation of messagequeue middleware ISMQ.

  15. Research on API Based on Visual Programming Platforms%基于可视化平台的 API 接口研究

    Institute of Scientific and Technical Information of China (English)

    关玉蓉

    2015-01-01

    In a variety of visual programming platforms, especially with the external network environment, , a large number of API functions under Windows can help reflect strong advanced programming skills proving to be a multiplier effect. This paper firstly introduces the API function and the dynamic link library DLL, then briefly ex-plains the classification of API functions, and focuses on how to call the API function and its application of API function in the visual platform. The paper finally describes the skills and notes to pay attention for users when call-ing the API function, which enables them to master the advanced programming skills of API interface.%在各种可视化编程平台中,尤其是与外部网络环境相结合,为了体现强大的高级编程能力,可用Windows系统提供的大量API函数,必然会起到事半功倍的效果。本文首先介绍了API函数以及动态链接库DLL,其次简要说明了API函数的分类,然后重点分析了在可视化平台中如何调用API函数及其应用API函数的高级编程能力,最后阐述了用户调用API函数的技巧和注意事项,以提高用户灵活掌握API接口的高级编程应用能力。

  16. API5 confers cancer stem cell-like properties through the FGF2-NANOG axis

    Science.gov (United States)

    Song, K-H; Cho, H; Kim, S; Lee, H-J; Oh, S J; Woo, S R; Hong, S-O; Jang, H S; Noh, K H; Choi, C H; Chung, J-Y; Hewitt, S M; Kim, J-H; Son, M; Kim, S-H; Lee, B I; Park, H-C; Bae, Y-K; Kim, T W

    2017-01-01

    Immune selection drives the evolution of tumor cells toward an immune-resistant and cancer stem cell (CSC)-like phenotype. We reported that apoptosis inhibitor-5 (API5) acts as an immune escape factor, which has a significant role in controlling immune resistance to antigen-specific T cells, but its functional association with CSC-like properties remains largely unknown. In this study, we demonstrated for the first time that API5 confers CSC-like properties, including NANOG expression, the frequency of CD44-positive cells and sphere-forming capacity. Critically, these CSC-like properties mediated by API5 are dependent on FGFR1 signaling, which is triggered by E2F1-dependent FGF2 expression. Furthermore, we uncovered the FGF2-NANOG molecular axis as a downstream component of API5 signaling that is conserved in cervical cancer patients. Finally, we found that the blockade of FGFR signaling is an effective strategy to control API5high human cancer. Thus, our findings reveal a crucial role of API5 in linking immune resistance and CSC-like properties, and provide the rationale for its therapeutic application for the treatment of API5+ refractory tumors. PMID:28092370

  17. Apoptosis inhibitor 5 (API-5; AAC-11; FIF) is upregulated in human carcinomas in vivo.

    Science.gov (United States)

    Koci, Lenka; Chlebova, Katarina; Hyzdalova, Martina; Hofmanova, Jirina; Jira, Miroslav; Kysela, Petr; Kozubik, Alois; Kala, Zdenek; Krejci, Pavel

    2012-04-01

    Apoptosis inhibitor 5 (API-5) is a 55 kDa nuclear protein with potent anti-apoptotic signaling in tumor cells in vitro. In this study, we analyzed the expression of the API-5 protein in vivo in a broad spectrum of human carcinomas, including those of the colon, lung, liver, kidney, pancreas, stomach and esophagus using tumor tissues obtained during tumor resection. The results showed significant upregulation of API-5 expression in biopsies of lung (23%, n=13) and colorectal tumors (33%, n=27) in comparison with biopsies from the adjacent normal tissue. Colon cancer biopsies were used to study the cell populations with an upregulated level of expression of API-5 more closely. Using a magnetic bead-based selection for the epithelial cell marker EpCAM, we purified epithelial cells from the tumor and control tissues and analyzed these cells for API-5 expression by western immunoblotting. We observed that EpCAM-positive tumor cells expressed API-5 in all three colorectal cancer cases tested, in contrast to the control EpCAM-positive and EpCAM-negative cells isolated from the control or tumor tissues. These data suggest that the expression of the API-5 protein is upregulated in tumor epithelial cells and may serve as a prognostic marker in colorectal cancer.

  18. api, A novel Medicago truncatula symbiotic mutant impaired in nodule primordium invasion.

    Science.gov (United States)

    Teillet, Alice; Garcia, Joseph; de Billy, Françoise; Gherardi, Michèle; Huguet, Thierry; Barker, David G; de Carvalho-Niebel, Fernanda; Journet, Etienne-Pascal

    2008-05-01

    Genetic approaches have proved to be extremely useful in dissecting the complex nitrogen-fixing Rhizobium-legume endosymbiotic association. Here we describe a novel Medicago truncatula mutant called api, whose primary phenotype is the blockage of rhizobial infection just prior to nodule primordium invasion, leading to the formation of large infection pockets within the cortex of noninvaded root outgrowths. The mutant api originally was identified as a double symbiotic mutant associated with a new allele (nip-3) of the NIP/LATD gene, following the screening of an ethylmethane sulphonate-mutagenized population. Detailed characterization of the segregating single api mutant showed that rhizobial infection is also defective at the earlier stage of infection thread (IT) initiation in root hairs, as well as later during IT growth in the small percentage of nodules which overcome the primordium invasion block. Neither modulating ethylene biosynthesis (with L-alpha-(2-aminoethoxyvinylglycine or 1-aminocyclopropane-1-carboxylic acid) nor reducing ethylene sensitivity in a skl genetic background alters the basic api phenotype, suggesting that API function is not closely linked to ethylene metabolism or signaling. Genetic mapping places the API gene on the upper arm of the M. truncatula linkage group 4, and epistasis analyses show that API functions downstream of BIT1/ERN1 and LIN and upstream of NIP/LATD and the DNF genes.

  19. Investigation into process-induced de-aggregation of cohesive micronised API particles.

    Science.gov (United States)

    Hoffmann, Magnus; Wray, Patrick S; Gamble, John F; Tobyn, Mike

    2015-09-30

    The aim of this study was to assess the impact of unit processes on the de-aggregation of a cohesive micronised API within a pharmaceutical formulation using near-infrared chemical imaging. The impact on the primary API particles was also investigated using an image-based particle characterization system with integrated Raman analysis. The blended material was shown to contain large, API rich domains which were distributed in-homogeneously across the sample, suggesting that the blending process was not aggressive enough to disperse aggregates of micronised drug particles. Cone milling, routinely used to improve the homogeneity of such cohesive formulations, was observed to substantially reduce the number and size of API rich domains; however, several smaller API domains survived the milling process. Conveyance of the cone milled formulation through the Alexanderwerk WP120 powder feed system completely dispersed all remaining aggregates. Importantly, powder feed transmission of the un-milled formulation was observed to produce an equally homogeneous API distribution. The size of the micronised primary drug particles remained unchanged during powder feed transmission. These findings provide further evidence that this powder feed system does induce shear, and is in fact better able to disperse aggregates of a cohesive micronised API within a blend than the blend-mill-blend step.

  20. EDGE REMOVAL OF 3D POLYGONAL MODEL USING MAYA API

    Directory of Open Access Journals (Sweden)

    SAMEER ARORA

    2010-09-01

    Full Text Available In various applications of computer graphics, 3D polygonal modeling is used, which consists millions of triangular polygon. In this polygon attributes – vertices, edges and faces’ details are to be stored. In order to control the processing time, storing space, and transfer speed, it is often required to reduce the information ofthese polygonal 3D models. In this paper an effort is made to reduce the number of edges. There are various methods to reduce faces and edges of these 3D models. A C++ dynamic link library as Maya Plugin has been created to remove number of edges of 3D triangular polygon model using the Quadric Error Metrics (QEM in MAYA v2010 x64 API. QEM allows fast and accurate geometric simplification of 3D models.

  1. AES Encryption and Decryption Using Direct3D 10 API

    Directory of Open Access Journals (Sweden)

    Adrian Marius Chiuta

    2011-06-01

    Full Text Available Current video cards (GPUs – Graphics Processing Units are very programmable, have become much more powerful than the CPUs and they are very affordable. In this paper, we present an implementation for the AES algorithm using Direct3D 10 certified GPUs. The graphics API Direct3D 10 is the first version that allows the use of integer operations, making from the traditional GPUs (that works only with floating point numbers, General Purpose GPUs that can be used for a large number of algorithms, including encryption. We present the performance of the symmetric key encryption algorithm – AES, on a middle range GPU and on a middle range quad core CPU. On the testing system, the developed solution is almost 3 times faster on the GPU than on one single core CPU, showing that the GPU can perform as an efficient cryptographic accelerator.

  2. API, Cloud computing, WebGIS and cartography

    Directory of Open Access Journals (Sweden)

    Andrea Favretto

    2013-05-01

    Full Text Available This paper explores some of the digital mapping processes available on the Internet in order to analyse their cartographic congruence. It will focus on WebGIS-based cartography in relation to what is produced using Mash-up site maps. These websites often use Googlebased maps in order to produce their own cartography. Thus, we can identify two main typologies of Internet mapping sites, which are characterized by the ownership or non-ownership of their cartographic bases. This paper will critically assess the cartography employed in the two different instances. A concise introduction to the Cloud Computing Internet propagated phenomenon is also premised in order to provide the reader with an accurate frame of reference. Cloud Computing has encouraged a significant Internet participation via the Application Programming Interface software (API, leading to mash-up cartographic websites.

  3. Pheromonal contest between honeybee workers ( Apis mellifera capensis)

    Science.gov (United States)

    Moritz, R. F. A.; Simon, U. E.; Crewe, R. M.

    2000-10-01

    Queenless workers of the Cape honeybee ( Apis mellifera capensis) can develop into reproductives termed pseudoqueens. Although they morphologically remain workers they become physiologically queenlike, produce offspring, and secrete mandibular gland pheromones similar to those of true queens. However, after queen loss only very few workers gain pseudoqueen status. A strong intracolonial selection governs which workers start oviposition and which remain sterile. The "queen substance", 9-keto-2(E)-decenoic acid (9-ODA), the dominant compound of the queen's mandibular gland pheromones, suppresses the secretion of queenlike mandibular gland pheromones in workers. It may act as an important signal in pseudoqueen selection. By analysing the mandibular gland pheromones of workers kept in pairs, we found that A. m. capensis workers compete to produce the strongest queen-like signal.

  4. A non-policing honey bee colony (Apis mellifera capensis)

    Science.gov (United States)

    Beekman, Madeleine; Good, Gregory; Allsopp, Mike; Radloff, Sarah; Pirk, Chris; Ratnieks, Francis

    2002-09-01

    In the Cape honey bee Apis mellifera capensis, workers lay female eggs without mating by thelytokous parthenogenesis. As a result, workers are as related to worker-laid eggs as they are to queen-laid eggs and therefore worker policing is expected to be lower, or even absent. This was tested by transferring worker- and queen-laid eggs into three queenright A. m. capensis discriminator colonies and monitoring their removal. Our results show that worker policing is variable in A. m. capensis and that in one colony worker-laid eggs were not removed. This is the first report of a non-policing queenright honey bee colony. DNA microsatellite and morphometric analysis suggests that the racial composition of the three discriminator colonies was different. The variation in policing rates could be explained by differences in degrees of hybridisation between A. m. capensis and A. m. scutellata, although a larger survey is needed to confirm this.

  5. Genetic structure of Apis mellifera macedonica in the Balkan Peninsula based on microsatellite DNA polymorphism

    DEFF Research Database (Denmark)

    Uzunov, Aleksandar; Meixner, Marina D; Kiprijanovska, Hrisula;

    2014-01-01

    The genetic variability of honey bees (Apis mellifera L.) from south eastern Europe was investigated using microsatellite analyses of 107 samples from Albania, the Republic of Macedonia, Greece and Bulgaria together with 42 reference samples (Apis mellifera carnica) from Slovenia. Genetic structure...... and spatial analyses of the microsatellite data showed a clear distinction between the Slovenian bees and all other populations, and confirmed the existence of Apis mellifera macedonica as an indigenous honey bee population in the regions that were sampled. In most areas however, varying degrees...

  6. Clarification of some api characteristics in relation to caribou (Rangifer tarandus

    Directory of Open Access Journals (Sweden)

    William O. Pruitt, Jr.

    1990-09-01

    Full Text Available A total of 2177 comparisons of api hardness vs. density in northern Saskatchewan, southeastern Manitoba and northeastern Finland revealed no consistent correlation (r varied from +.70 to -.17. A total of 1395 comparisons of horizontal hardness of the top layer of api to vertical hardness of the same layer of api in southeastern Manitoba, northeastern Finland and far eastern middle Finland revealed no consistent correlation (r varied from +.99 to -.20. Therefore one cannot substitute density for hardness nor horizontal hardness of the top layer for vertical hardness of the top layer in the terms of the Värriö Snow Index.

  7. Equilibrios de precipitación. Interacciones entre las reacciones de complejación y la solubilidad. Estudio de la solubilización de los haluros de plata en presencia del complejante amoníaco. Ejercicio interactivo.

    OpenAIRE

    Milla González, Miguel

    2015-01-01

    Es necesario encontrar en este ejercicio la concentración de amoníaco necesaria para impedir la precipitación de los haluros de plata AgCl, AgBr y AgI. Se demuestra, entre otras conclusiones, que el último de estos tres es insoluble en amoníaco y que esta sustancia no impide la precipitación de la sal poco soluble AgI.

  8. Valvoplastia mitral percutânea complicada com tamponamento cardíaco em paciente gestante Percutaneous mitral valvuloplasty complicated by cardiac tamponade in a pregnant patient

    Directory of Open Access Journals (Sweden)

    Luís Seca

    2008-11-01

    Full Text Available A ocorrência de Hemopericárdio com Tamponamento Cardíaco é uma complicação da Valvoplastia Mitral Percutânea por balão (VMB, que apesar de pouco freqüente, pode ter graves conseqüências materno-fetais. Este artigo descreve o caso de um tamponamento cardíaco após VMB em uma mulher de 28 anos, com estenose mitral reumática severa na 20ª semana de gravidez.The occurrence of hemopericardium with cardiac tamponade is a complication of percutaneous balloon mitral valvuloplasty (BMV which, although uncommon, may have severe maternal and fetal consequences. This article describes a case of cardiac tamponade following BMV in a 28-year-old woman with severe rheumatic mitral stenosis in the 20th week of gestation.

  9. Angiosarcomas cardíacos. Implicación diagnóstica, pronóstica y terapéutica

    OpenAIRE

    Luciana Schocron; Sebastián Schanz; Edgardo Beck

    2007-01-01

    Los tumores cardíacos primarios constituyen una entidad rara. Sólo el 10% a 25% de ellos son malignos y el angiosarcoma es el más frecuente. Ocasionan sintomatología cardíaca y sistémica variada, por sí mismos o por compromiso de otros órganos, lo que puede simular otras patologías y derivar en un retraso del diagnóstico de certeza.Se describen dos casos de angiosarcoma cardíaco con presentaciones atípicas, por lo que inicialmente no se consideró esta patología como un diagnóstico probable.Se...

  10. The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space

    Directory of Open Access Journals (Sweden)

    Schiller Madlen

    2012-07-01

    Full Text Available Abstract Background The two highly similar Arabidopsis apyrases AtAPY1 and AtAPY2 were previously shown to be involved in plant growth and development, evidently by regulating extracellular ATP signals. The subcellular localization of AtAPY1 was investigated to corroborate an extracellular function. Results Transgenic Arabidopsis lines expressing AtAPY1 fused to the SNAP-(O6-alkylguanine-DNA alkyltransferase-tag were used for indirect immunofluorescence and AtAPY1 was detected in punctate structures within the cell. The same signal pattern was found in seedlings stably overexpressing AtAPY1-GFP by indirect immunofluorescence and live imaging. In order to identify the nature of the AtAPY1-positive structures, AtAPY1-GFP expressing seedlings were treated with the endocytic marker stain FM4-64 (N-(3-triethylammoniumpropyl-4-(p-diethylaminophenyl-hexatrienyl-pyridinium dibromide and crossed with a transgenic line expressing the trans-Golgi marker Rab E1d. Neither FM4-64 nor Rab E1d co-localized with AtAPY1. However, live imaging of transgenic Arabidopsis lines expressing AtAPY1-GFP and either the fluorescent protein-tagged Golgi marker Membrin 12, Syntaxin of plants 32 or Golgi transport 1 protein homolog showed co-localization. The Golgi localization was confirmed by immunogold labeling of AtAPY1-GFP. There was no indication of extracellular AtAPY1 by indirect immunofluorescence using antibodies against SNAP and GFP, live imaging of AtAPY1-GFP and immunogold labeling of AtAPY1-GFP. Activity assays with AtAPY1-GFP revealed GDP, UDP and IDP as substrates, but neither ATP nor ADP. To determine if AtAPY1 is a soluble or membrane protein, microsomal membranes were isolated and treated with various solubilizing agents. Only SDS and urea (not alkaline or high salt conditions were able to release the AtAPY1 protein from microsomal membranes. Conclusions AtAPY1 is an integral Golgi protein with the substrate specificity typical for Golgi apyrases. It is

  11. IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology.

    Science.gov (United States)

    Van Vaerenbergh, Matthias; De Smet, Lina; Rafei-Shamsabadi, David; Blank, Simon; Spillner, Edzard; Ebo, Didier G; Devreese, Bart; Jakob, Thilo; de Graaf, Dirk C

    2015-02-01

    Api m 10 has recently been established as novel major allergen that is recognized by more than 60% of honeybee venom (HBV) allergic patients. Previous studies suggest Api m 10 protein heterogeneity which may have implications for diagnosis and immunotherapy of HBV allergy. In the present study, RT-PCR revealed the expression of at least nine additional Api m 10 transcript isoforms by the venom glands. Two distinct mechanisms are responsible for the generation of these isoforms: while the previously known variant 2 is produced by an alternative splicing event, novel identified isoforms are intragenic chimeric transcripts. To the best of our knowledge, this is the first report of the identification of chimeric transcripts generated by the honeybee. By a retrospective proteomic analysis we found evidence for the presence of several of these isoforms in the venom proteome. Additionally, we analyzed IgE reactivity to different isoforms by protein array technology using sera from HBV allergic patients, which revealed that IgE recognition of Api m 10 is both isoform- and patient-specific. While it was previously demonstrated that the majority of HBV allergic patients display IgE reactivity to variant 2, our study also shows that some patients lacking IgE antibodies for variant 2 display IgE reactivity to two of the novel identified Api m 10 variants, i.e. variants 3 and 4.

  12. Ecocardiografia sob estresse físico e eventos cardíacos maiores em pacientes com ergometria normal

    Directory of Open Access Journals (Sweden)

    Flávia Ricci Calasans

    2013-07-01

    Full Text Available FUNDAMENTO: O teste ergométrico (TE, inicialmente indicado para diagnóstico e estratificação de risco da doença arterial coronariana (DAC, apresenta baixa sensibilidade, o que pode implicar em falha na detecção de pacientes com risco aumentado de eventos adversos. OBJETIVO: Avaliar a ecocardiografia sob estresse físico (EEF na predição de eventos cardíacos maiores (ECM e óbitos por todas as causas em pacientes com probabilidade pré-teste intermediária de DAC e TE normal. MÉTODO: Trata-se de estudo retrospectivo em que foram estudados 397 pacientes, com probabilidade pré-teste intermediária de DAC pelo método de Morise e TE normal, submetidos à EEF. Dividiu-se em dois grupos: EEF negativo (G1 ou positivo (G2 para isquemia miocárdica. Os desfechos avaliados foram mortalidade por qualquer causa e ECM, definidos como óbito cardíaco e infarto agudo do miocárdio (IAM não fatal. RESULTADOS: O grupo G1 constituiu-se de 329 (82,8% pacientes. A idade média foi de 57,37 ± 11 anos e 44,1% eram do gênero masculino. Durante o seguimento médio de 75,94 ± 17,24 meses, ocorreram 13 óbitos, dentre eles três por causas cardíacas (IAM, além de 13 IAM não fatais. Isquemia miocárdica permaneceu como preditor independente de ECM (RR 2,49; [IC] 95% 1,74-3,58. As variáveis preditoras de mortalidade por qualquer causa foram gênero masculino (RR 9,83; [IC] 95% 2,15-44,97 e idade > 60 anos (RR 4,57; [IC] 95% 1,39-15,23. CONCLUSÃO: A EEF positiva é preditora de ECM na amostra estudada, o que auxilia na identificação de um subgrupo de pacientes sob maior risco de eventos adversos, apesar de apresentarem TE normal.

  13. Sildenafil vs. Nitroprussiato de Sódio durante Teste de Reatividade Pulmonar pré-transplante cardíaco

    Directory of Open Access Journals (Sweden)

    Aguinaldo Figueiredo Freitas Jr

    2012-09-01

    Full Text Available FUNDAMENTO: A hipertensão pulmonar é associada ao pior prognóstico no pós-transplante cardíaco. O teste de reatividade pulmonar com Nitroprussiato de Sódio (NPS está associado a elevados índices de hipotensão arterial sistêmica, disfunção ventricular do enxerto transplantado e elevadas taxas de desqualificação para o transplante. OBJETIVO: Neste estudo, objetivou-se comparar os efeitos do Sildenafil (SIL e NPS sobre variáveis hemodinâmicas, neuro-hormonais e ecocardiográficas durante teste de reatividade pulmonar. MÉTODOS: Os pacientes foram submetidos, simultaneamente, ao cateterismo cardíaco direito, ao ecocardiograma e à dosagem de BNP e gasometria venosa, antes e após administração de NPS (1 - 2 µg/Kg/min ou SIL (100 mg, dose única. RESULTADOS: Ambos reduziram a hipertensão pulmonar, porém o nitrato promoveu hipotensão sistêmica significativa (Pressão Arterial Média - PAM: 85,2 vs. 69,8 mmHg, p < 0,001. Ambos reduziram as dimensões cardíacas e melhoraram a função cardíaca esquerda (NPS: 23,5 vs. 24,8 %, p = 0,02; SIL: 23,8 vs. 26 %, p < 0,001 e direita (SIL: 6,57 ± 2,08 vs. 8,11 ± 1,81 cm/s, p = 0,002; NPS: 6,64 ± 1,51 vs. 7,72 ± 1,44 cm/s, p = 0,003, medidas pela fração de ejeção ventricular esquerda e Doppler tecidual, respectivamente. O SIL, ao contrário do NPS, apresentou melhora no índice de saturação venosa de oxigênio, medido pela gasometria venosa. CONCLUSÃO: Sildenafil e NPS são vasodilatadores que reduzem, de forma significativa, a hipertensão pulmonar e a geometria cardíaca, além de melhorar a função biventricular. O NPS, ao contrário do SIL, esteve associado a hipotensão arterial sistêmica e piora da saturação venosa de oxigênio.

  14. El calcio en los miocitos cardíacos y su papel en las miocardiopatías.

    Directory of Open Access Journals (Sweden)

    Guido Ulate Montero

    2006-01-01

    Full Text Available El papel que desempeña el calcio en los miocitos cardíacos abarca un gran número de funciones, desde su rol en el acoplamiento excitación-ccontracción hasta su papel de segundo mensajero en las diversas vías de señalización, algunas de las cuales se activan en procesos que afectan la integridad del tejido miocárdico y que tienen que ver con el crecimiento y la apoptosis de los miocitos y que al final, son los que determinan la evolución de la mayoría de las cardiomiopatías. En esta revisión se abordan los mecanismos fisiológicos en las células de músculo cardíaco en los que este ión juega un papel determinante y los cambios que se presentan en ciertas cardiopatías como la miocardiopatía arritmogénica del ventrículo derecho, las miocardiopatías asociadas a alteraciones en el receptor de rianodina y la miocardiopatía dilatada. Se revisa también la farmacodinamia de los agentes inotrópicos positivos que actúan sensibilizando los miofilamentos del sarcómero al calcio.The rol played by calcium in cardiomyocytes includes a great number of functions like the excitation-contraction coupling as well as a second messenger in diverse signaling pathways. Some of these pathways are activated in processes that affect the cardiac tissue integrity and also participate in cell growth and apoptosis, determining the poor prognosis that characterize the majority of the cardiomyopathies. In this review,the mechanisms inside the cardiomyocytes in which participates calcium and the pathophysiological changes observed in some cardiac diseases like arrhythmogenic right ventricular cardiomyopathy, catecholaminergic polymorphic ventricular tachycardia, idiopathic dilated cardiomyopathy, and others, are described. The action mechanism of positive inotropic drugs that act as sarcomere myofilaments sensitizers is also reviewed.

  15. Histochemical Comparison of the Hypopharyngeal Gland in Apis cerana Fabricius, 1793 Workers and Apis mellifera Linnaeus, 1758 Workers

    Directory of Open Access Journals (Sweden)

    Guntima Suwannapong

    2010-01-01

    Full Text Available Hypopharyngeal glands of honeybee are age-dependent structures that change with the size of acini and are correlated with various social behaviors. The histochemical structure of Apis cerana and A. mellifera worker hypopharyngeal glands in four different developmental stages wes stained with ninhydrin Schiff's and periodic acid Schiff's reagents (PAS for localization of proteins and carbohydrates, respectively, and examined with light microscopy. Nurse bees of both honeybee species had significantly larger glands as compared to guards and forgers, but there were no statistically significant differences between these two species after accounting for caste. Gland protein concentration increased progressively in nurse bees, and this was correlated with the appearance of enriched protein granules in the cytoplasm. In addition, the hypopharyngeal gland protein concentration of A. mellifera was higher than that of A. cerana even though gland size was not significantly different between species. However, gland size was shown to have decreased progressively in foragers and guards.

  16. Homology differences between complete Sacbrood virus genomes from infected Apis mellifera and Apis cerana honeybees in Korea.

    Science.gov (United States)

    Reddy, Kondreddy Eswar; Yoo, Mi Sun; Kim, Young-Ha; Kim, Nam-Hee; Ramya, Mummadireddy; Jung, Ha-Na; Thao, Le Thi Bich; Lee, Hee-Soo; Kang, Seung-Won

    2016-04-01

    Sacbrood virus (SBV) represents a serious threat to the health of managed honeybees. We determined four complete SBV genomic sequences (AmSBV-Kor1, AmSBV-Kor2, AcSBV-Kor3, and AcSBV-Kor4) isolated from Apis mellifera and Apis cerana in various regions of South Korea. A phylogenetic tree was constructed from the complete genomic sequences of these Korean SBVs (KSBVs) and 21 previously reported SBV sequences from other countries. Three KSBVs (not AmSBV-Kor1) clustered with previously reported Korean genomes, but separately from SBV genomes from other countries. The KSBVs shared 90-98 % identity, and 89-97 % identity with the genomes from other countries. AmSBV-Kor1 was least similar (~90 % identity) to the other KSBVs, and was most similar to previously reported strains AmSBV-Kor21 (97 %) and AmSBV-UK (93 %). Phylogenetic analysis of the partial VP1 region sequences indicated that SBVs clustered by host species and country of origin. The KSBVs were aligned with nine previously reported complete SBV genomes and compared. The KSBVs were most different from the other genomes at the end of the 5' untranslated region and in the entire open reading frame. A SimPlot graph of the VP1 region confirmed its high variability, especially between the SBVs infecting A. mellifera and A. cerana. In this genomic region, SBVs from A. mellifera species contain an extra continuous 51-nucleotide sequence relative to the SBVs from A. cerana. This genomic diversity may reflect the adaptation of SBV to specific hosts, viral cross-infections, and the spatial distances separating the KSBVs from other SBVs.

  17. Telephone Interpreter Services (TIS)-Asian and Pacific Islander (API) Language Fiscal Year Quarterly Dataset

    Data.gov (United States)

    Social Security Administration — This dataset displays our quarterly national TIS call volume for over 45 API languages for the current fiscal year. A fiscal year runs from October through September...

  18. Telephone Interpreter Services (TIS)-Asian and Pacific Islander (API) Language Yearly Dataset

    Data.gov (United States)

    Social Security Administration — This dataset displays our national TIS call volume for over 45 API languages for the last three fiscal years. A fiscal year runs from October through September. We...

  19. Bilingual education, indigenous language and culture: the case of Apyãwa Tapirapé

    Directory of Open Access Journals (Sweden)

    Maria Gorete Neto

    Full Text Available Bilingual schools are recognized as an important resource to increase the chances of language survival but in many cases have proven to be ineffective. Within the Apyãwa Tapirapé Indian Tribe (central Brazil, an effective bilingual school does exist; however, this study shows that even a successful school brings complications for the community. Audio-recorded interviews, in which teachers and leaders discuss their bilingual school and its consequences for the Apyãwa Tapirapé people, reveal that they feel that the school has changed the Apyãwa Tapirapé lifestyle in both negative and positive ways. A continuous evaluation and ongoing reconstruction of educational aspects is proposed as a way to both attend to the needs and to relieve the worries of groups like the Apyãwa Tapirapé with respect to the impact of their school on the community.

  20. A review on the taste masking of bitter APIs: hot-melt extrusion (HME) evaluation.

    Science.gov (United States)

    Maniruzzaman, Mohammed; Boateng, Joshua S; Chowdhry, Babur Z; Snowden, Martin J; Douroumis, Dennis

    2014-02-01

    The majority of active pharmaceutical ingredients (APIs) found in oral dosage forms have a bitter taste. Masking the unpleasant taste of bitter, APIs is a major challenge in the development of such oral dosage forms. Taste assessment is an important quality-control parameter for evaluating taste-masked formulations of any new molecular entity. Hot-melt extrusion (HME) techniques, have very recently, been accepted from an industrial compliance viewpoint in relation to both manufacturing operations and development of pharmaceuticals. HME achieves taste masking of bitter APIs via various mechanisms such as the formation of solid dispersions and inter-molecular interactions and this has led to its wide-spread use in pharmaceutical formulation research. In this article, the uses of various taste evaluation methods and HME as continuous processing techniques for taste masking of bitter APIs used for the oral delivery of drugs are reviewed.

  1. Candida albicans response to spaceflight (NASA STS-115) --- GSM1231690_Slide_43 API

    Data.gov (United States)

    National Aeronautics and Space Administration — A fully queryable REST API with JSON, XML, and CSV output as well as inline, runable examples using data from the transcriptional profiling and phenotypic...

  2. Dosis Letal Media (DL50 de algunos aceites esenciales y biocidas efectivos para el control de Ascosphaera apis en Apis mellifera L. - Median Lethal Dose (LD50 of some essential oils and biocides effective for the control of Ascosphaera apis on Apis mellifera L

    Directory of Open Access Journals (Sweden)

    Albo, Graciela N

    2010-10-01

    Full Text Available ResumenLa ascofaeriosis o cría yesificada, es una enfermedad de la abeja melífera (Apis mellifera L. causada por el hongo Ascosphaera apis que produce la momificación de las larvas. Los aceites esenciales poseen efectos antimicrobianos y han sido utilizados para el control de cría yesificada. Por otra parte, a nivel mundial se han probado gran cantidad de productos de síntesis, pero ninguno ha resultado 100 % eficaz. SummaryChalkbrood is a disease of the honeybee (Apis mellifera L caused by the fungus Ascosphaera apis that transforms larvae into mummies. Essential oils have showed antimicrobial activity and have been used in the control of chalkbrood. Moreover, world-wide, great number of synthesized products have been assayed but no one has resulted 100% effective.

  3. S E- GEOD-50881 Study Samples --- Candida albicans response to spaceflight (NASA STS-115) API

    Data.gov (United States)

    National Aeronautics and Space Administration — A fully queryable REST API with JSON, XML, and CSV output as well as inline, runable examples using data from the transcriptional profiling and phenotypic...

  4. A E- GEOD-50881 Gene Chip Assay --- Candida albicans response to spaceflight (NASA STS-115) API

    Data.gov (United States)

    National Aeronautics and Space Administration — A fully queryable REST API with JSON, XML, and CSV output as well as inline, runable examples using data from the transcriptional profiling and phenotypic...

  5. BUILDING MODEL ANALYSIS APPLICATIONS WITH THE JOINT UNIVERSAL PARAMETER IDENTIFICATION AND EVALUATION OF RELIABILITY (JUPITER) API

    Science.gov (United States)

    The open-source, public domain JUPITER (Joint Universal Parameter IdenTification and Evaluation of Reliability) API (Application Programming Interface) provides conventions and Fortran-90 modules to develop applications (computer programs) for analyzing process models. The input ...

  6. Three-dimensional model of the honeybee venom allergen Api m 7: structural and functional insights.

    Science.gov (United States)

    Georgieva, Dessislava; Greunke, Kerstin; Betzel, Christian

    2010-06-01

    Api m 7 is one of the major protease allergens of the honeybee venom. It consists of a serine protease-like (SPL) and a CUB domain. The knowledge about the structure and function of Api m 7 is limited mainly to its amino acid sequence. Three-dimensional models of the two structural domains were constructed using their amino acid sequences and the crystallographic coordinates of prophenoloxidase-activating factor (PPAF-II) as a template for the SPL domain and the coordinates of porcine spermadhesin PSP-II for the CUB domain. The structural organization of Api m 7 suggests that the CUB domain is involved in interactions with natural substrates while the SPL domain probably activates zymogens. IgE epitopes and antigenic sites were predicted. Api m 7 shows structural and functional similarity to the members of the PPAF-II family. Possible substrates, function and evolution of the enzyme are discussed in the paper.

  7. Swarm Intelligence Based Ant Colony Optimization (ACO Approach for Maximizing the Lifetime of Heterogeneous Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    LingaRaj.K

    2013-11-01

    Full Text Available This paper attempts to undertake the study of maximizing the lifetime of Heterogeneous wireless sensor networks (WSNs. In wireless sensor networks, sensor nodes are typically power-constrained with limited lifetime, and thus it is necessary to know how long the network sustains its networking operations. Heterogeneous WSNs consists of different sensor devices with different capabilities. We can enhance the quality of monitoring in wireless sensor networks by increasing the coverage area. One of major issue in WSNs is finding maximum number of connected coverage. This paper proposed a Swarm Intelligence, Ant Colony Optimization (ACO based approach. Ant colony optimization algorithm provides a natural and intrinsic way of exploration of search space of coverage area. Ants communicate with their nest- mates using chemical scents known as pheromones, Based on Pheromone trail between sensor devices the shortest path is found. The methodology is based on finding the maximum number of connected covers that satisfy both sensing coverage and network connectivity. By finding the coverage area and sensing range, the network lifetime maximized and reduces the energy consumption

  8. The Norma Cluster (ACO 3627): I. A Dynamical Analysis of the Most Massive Cluster in the Great Attractor

    CERN Document Server

    Woudt, P A; Lucey, J; Fairall, A P; Moore, S A W

    2007-01-01

    A detailed dynamical analysis of the nearby rich Norma cluster (ACO 3627) is presented. From radial velocities of 296 cluster members, we find a mean velocity of 4871 +/- 54 km/s and a velocity dispersion of 925 km/s. The mean velocity of the E/S0 population (4979 +/- 85 km/s) is offset with respect to that of the S/Irr population (4812 +/- 70 km/s) by `Delta' v = 164 km/s in the cluster rest frame. This offset increases towards the core of the cluster. The E/S0 population is free of any detectable substructure and appears relaxed. Its shape is clearly elongated with a position angle that is aligned along the dominant large-scale structures in this region, the so-called Norma wall. The central cD galaxy has a very large peculiar velocity of 561 km/s which is most probably related to an ongoing merger at the core of the cluster. The spiral/irregular galaxies reveal a large amount of substructure; two dynamically distinct subgroups within the overall spiral-population have been identified, located along the Nor...

  9. Antagonistic Effect of Gut Bacteria in the Hybrid Carniolan Honey Bee, Apis Mellifera Carnica, Against Ascosphaera Apis, the Causal Organism of Chalkbrood Disease

    Directory of Open Access Journals (Sweden)

    Omar Mohamed O. M.

    2014-06-01

    Full Text Available The objective of this study was to isolate and characterize bacterial strains associated with the gut of the hybrid Carniolan honey bee, Apis mellifera carnica, and to determine their in vitro and in vivo potential against Ascosphaera apis, the causal organism of chalkbrood disease, with the purpose of exploring feasible biological control. Six bacterial strains were isolated from healthy worker honey bees by culture-dependent methods. Six fungal strains (A3, A4, A7, A8, A9, and A15 of A. apis were isolated from larvae suffering from chalkbrood disease on Yeast-Glucose-Starch agar (YGPSA medium. All bacteria were identified by a combination of morphology, Gram stain, and 16S rRNA sequence analysis, and fungal strains were identified by morphology and 5.8S rRNA. In vitro and in vivo inhibition assays were carried out to determine the ability of bacterial isolates to inhibit A. apis, the causal agent of chalkbrood disease. The analysis of 16S rRNA sequences revealed that four bacterial strains (B2, B4, B10, and B100 belong to Bacillus subtilis species, and two strains (P1 and P5 belong to Pseudomonas fluorescence. Significant differences in antagonistic activity of all bacterial strains were observed. B. subtilis isolate B2 showed the highest antagonistic activity, as measured by the inhibition zone against A. apis, followed by the P1 strain of P. fluorescence. SEM analysis also supports the antagonistic activity of these bacteria against A. apis. This study provides a theoretical basis for biological control of honey bee chalkbrood disease.

  10. Revoke and Let Live: A Secure Key Revocation API for Cryptographic Devices

    OpenAIRE

    Cortier, Véronique; Steel,Graham; Wiedling, Cyrille

    2012-01-01

    While extensive research addresses the problem of establishing session keys through cryptographic protocols, relatively little work has appeared addressing the problem of revocation and update of long term keys. We present an API for symmetric key management on embedded devices that supports revocation and prove security properties design in the symbolic model of cryptography. Our API supports two modes of revocation: a passive mode where keys have an expiration time, and an active mode where...

  11. Sensitivity Analysis of Fatigue Crack Growth Model for API Steels in Gaseous Hydrogen

    Science.gov (United States)

    Amaro, Robert L; Rustagi, Neha; Drexler, Elizabeth S; Slifka, Andrew J

    2014-01-01

    A model to predict fatigue crack growth of API pipeline steels in high pressure gaseous hydrogen has been developed and is presented elsewhere. The model currently has several parameters that must be calibrated for each pipeline steel of interest. This work provides a sensitivity analysis of the model parameters in order to provide (a) insight to the underlying mathematical and mechanistic aspects of the model, and (b) guidance for model calibration of other API steels. PMID:26601024

  12. Sensitivity Analysis of Fatigue Crack Growth Model for API Steels in Gaseous Hydrogen.

    Science.gov (United States)

    Amaro, Robert L; Rustagi, Neha; Drexler, Elizabeth S; Slifka, Andrew J

    2014-01-01

    A model to predict fatigue crack growth of API pipeline steels in high pressure gaseous hydrogen has been developed and is presented elsewhere. The model currently has several parameters that must be calibrated for each pipeline steel of interest. This work provides a sensitivity analysis of the model parameters in order to provide (a) insight to the underlying mathematical and mechanistic aspects of the model, and (b) guidance for model calibration of other API steels.

  13. A RESTful API for accessing microbial community data for MG-RAST.

    Science.gov (United States)

    Wilke, Andreas; Bischof, Jared; Harrison, Travis; Brettin, Tom; D'Souza, Mark; Gerlach, Wolfgang; Matthews, Hunter; Paczian, Tobias; Wilkening, Jared; Glass, Elizabeth M; Desai, Narayan; Meyer, Folker

    2015-01-01

    Metagenomic sequencing has produced significant amounts of data in recent years. For example, as of summer 2013, MG-RAST has been used to annotate over 110,000 data sets totaling over 43 Terabases. With metagenomic sequencing finding even wider adoption in the scientific community, the existing web-based analysis tools and infrastructure in MG-RAST provide limited capability for data retrieval and analysis, such as comparative analysis between multiple data sets. Moreover, although the system provides many analysis tools, it is not comprehensive. By opening MG-RAST up via a web services API (application programmers interface) we have greatly expanded access to MG-RAST data, as well as provided a mechanism for the use of third-party analysis tools with MG-RAST data. This RESTful API makes all data and data objects created by the MG-RAST pipeline accessible as JSON objects. As part of the DOE Systems Biology Knowledgebase project (KBase, http://kbase.us) we have implemented a web services API for MG-RAST. This API complements the existing MG-RAST web interface and constitutes the basis of KBase's microbial community capabilities. In addition, the API exposes a comprehensive collection of data to programmers. This API, which uses a RESTful (Representational State Transfer) implementation, is compatible with most programming environments and should be easy to use for end users and third parties. It provides comprehensive access to sequence data, quality control results, annotations, and many other data types. Where feasible, we have used standards to expose data and metadata. Code examples are provided in a number of languages both to show the versatility of the API and to provide a starting point for users. We present an API that exposes the data in MG-RAST for consumption by our users, greatly enhancing the utility of the MG-RAST service.

  14. A RESTful API for accessing microbial community data for MG-RAST.

    Directory of Open Access Journals (Sweden)

    Andreas Wilke

    2015-01-01

    Full Text Available Metagenomic sequencing has produced significant amounts of data in recent years. For example, as of summer 2013, MG-RAST has been used to annotate over 110,000 data sets totaling over 43 Terabases. With metagenomic sequencing finding even wider adoption in the scientific community, the existing web-based analysis tools and infrastructure in MG-RAST provide limited capability for data retrieval and analysis, such as comparative analysis between multiple data sets. Moreover, although the system provides many analysis tools, it is not comprehensive. By opening MG-RAST up via a web services API (application programmers interface we have greatly expanded access to MG-RAST data, as well as provided a mechanism for the use of third-party analysis tools with MG-RAST data. This RESTful API makes all data and data objects created by the MG-RAST pipeline accessible as JSON objects. As part of the DOE Systems Biology Knowledgebase project (KBase, http://kbase.us we have implemented a web services API for MG-RAST. This API complements the existing MG-RAST web interface and constitutes the basis of KBase's microbial community capabilities. In addition, the API exposes a comprehensive collection of data to programmers. This API, which uses a RESTful (Representational State Transfer implementation, is compatible with most programming environments and should be easy to use for end users and third parties. It provides comprehensive access to sequence data, quality control results, annotations, and many other data types. Where feasible, we have used standards to expose data and metadata. Code examples are provided in a number of languages both to show the versatility of the API and to provide a starting point for users. We present an API that exposes the data in MG-RAST for consumption by our users, greatly enhancing the utility of the MG-RAST service.

  15. Using DLL as Interface between API an VC#.NET Applications

    Directory of Open Access Journals (Sweden)

    Marian DARDALA

    2006-01-01

    Full Text Available This paper presents a solution for using complex Win32API data structures and functions in Visual C# .NET applications. We built DLL (Dynamic Link Library to manage the API functions and data structures and we used DLL modules in a C# application. This is an easier working way compared with the traditional way of importing and managing API’s functions in C# programs.

  16. Qualidade de vida de pacientes submetidos ao transplante cardíaco: aplicação da escala Whoqol-Bref

    Directory of Open Access Journals (Sweden)

    Maria Isis Freire de Aguiar

    2011-01-01

    Full Text Available FUNDAMENTO: O sucesso do transplante cardíaco significa garantir a sobrevida dos pacientes com cardiopatia e permitir-lhes desenvolver suas atividades diárias. O transplante cardíaco apresenta-se como a primeira opção de tratamento na falência cardíaca, representando um aumento de sobrevida e qualidade de vida dos transplantados. OBJETIVO: Avaliar a qualidade de vida de pacientes submetidos ao transplante cardíaco através da aplicação de uma escala padronizada (Whoqol-Bref. MÉTODOS: Estudo exploratório descritivo de abordagem quantitativa, realizado com 55 pacientes submetidos ao transplante cardíaco, em um período entre o terceiro e o 103º mês, que realizam acompanhamento na Unidade de Transplante e Insuficiência Cardíaca em um Hospital de Referência em Cardiologia na cidade de Fortaleza, CE. Os dados foram coletados no período de fevereiro a abril de 2009, por meio da aplicação de um questionário padronizado pela Organização Mundial da Saúde e utilização de dados constantes nos prontuários. RESULTADOS: Com relação ao domínio físico, 62,8% e 58,3% dos pacientes, dos sexos masculino e feminino, respectivamente, estão satisfeitos. No domínio psicológico, dentre pacientes do sexo masculino, 65,1% apresentam satisfação quanto à qualidade de vida e, no sexo feminino, 58,3% encontram-se satisfeitas. No domínio das relações sociais, observou-se que, no sexo masculino, 53,5% estão muito satisfeitos, e apresentou-se um nível de satisfação de 100% no sexo feminino. No domínio do meio ambiente, 65,1% do sexo masculino encontram-se satisfeitos, e no sexo feminino, 83,3% estão satisfeitas. CONCLUSÃO: O transplante cardíaco teve bastante influência na qualidade de vida dos pacientes transplantados, pois os resultados mostram-se estatisticamente significantes no pós-transplante.

  17. Hot-stage microscopy for determination of API particles in a formulated tablet.

    Science.gov (United States)

    Simek, Michal; Grünwaldová, Veronika; Kratochvíl, Bohumil

    2014-01-01

    Although methods exist to readily determine the particle size distribution (PSD) of an active pharmaceutical ingredient (API) before its formulation into a final product, the primary challenge is to develop a method to determine the PSD of APIs in a finished tablet. To address the limitations of existing PSD methods, we used hot-stage microscopy to observe tablet disintegration during temperature change and, thus, reveal the API particles in a tablet. Both mechanical and liquid disintegration were evaluated after we had identified optimum milling time for mechanical disintegration and optimum volume of water for liquid disintegration. In each case, hot-stage micrographs, taken before and after the API melting point, were compared with image analysis software to obtain the PSDs. Then, the PSDs of the APIs from the disintegrated tablets were compared with the PSDs of raw APIs. Good agreement was obtained, thereby confirming the robustness of our methodology. The availability of such a method equips pharmaceutical scientists with an in vitro assessment method that will more reliably determine the PSD of active substances in finished tablets.

  18. The Matchmaker Exchange API: automating patient matching through the exchange of structured phenotypic and genotypic profiles.

    Science.gov (United States)

    Buske, Orion J; Schiettecatte, François; Hutton, Benjamin; Dumitriu, Sergiu; Misyura, Andriy; Huang, Lijia; Hartley, Taila; Girdea, Marta; Sobreira, Nara; Mungall, Chris; Brudno, Michael

    2015-10-01

    Despite the increasing prevalence of clinical sequencing, the difficulty of identifying additional affected families is a key obstacle to solving many rare diseases. There may only be a handful of similar patients worldwide, and their data may be stored in diverse clinical and research databases. Computational methods are necessary to enable finding similar patients across the growing number of patient repositories and registries. We present the Matchmaker Exchange Application Programming Interface (MME API), a protocol and data format for exchanging phenotype and genotype profiles to enable matchmaking among patient databases, facilitate the identification of additional cohorts, and increase the rate with which rare diseases can be researched and diagnosed. We designed the API to be straightforward and flexible in order to simplify its adoption on a large number of data types and workflows. We also provide a public test data set, curated from the literature, to facilitate implementation of the API and development of new matching algorithms. The initial version of the API has been successfully implemented by three members of the Matchmaker Exchange and was immediately able to reproduce previously identified matches and generate several new leads currently being validated. The API is available at https://github.com/ga4gh/mme-apis.

  19. An analysis of multifractal characteristics of API time series in Nanjing, China

    Science.gov (United States)

    Shen, Chen-hua; Huang, Yi; Yan, Ya-ni

    2016-06-01

    This paper describes multifractal characteristics of daily air pollution index (API) records in Nanjing from 2001 to 2012. The entire daily API time series is first divided into 12 parts that serve as research objects, and the generalized Hurst exponent is calculated for each series. And then, the multifractal sources are analyzed and singularity spectra are shown. Next, based on a singularity spectrum, the multifractal-characteristics parameters (maximum exponent α0, spectrum width Δ α, and asymmetry Δ αas) are introduced. The results show that the fractality of daily API for each year is multifractal. The multifractal sources originate from both a broad probability density function and different long-range correlations with small and large fluctuations. The strength of the distribution multifractality is stronger than that of the correlation multifractality. The variation in the structure of API time series with increasing years is mainly related to long-range correlations. The structure of API time series in some years is richer. These findings can provide a scientific basis for further probing into the complexity of API.

  20. Scaled-up bioconversion of fish waste to liquid fertilizer using a 5 L ribbon-type reactor.

    Science.gov (United States)

    Dao, Van Thingoc; Kim, Joong Kyun

    2011-10-01

    A scaled-up conversion process of fish waste to liquid fertilizer was performed in a 5 L ribbon-type reactor. Biodegradation was performed by inoculation of autoclaved fish waste with 5.84 × 10(5) CFU mL(-1) of mixed microorganisms for 96 h. As a result, the pH changed from 6.92 to 5.72, the cell number reached 7.28 × 10(5) CFU mL(-1), and approximately 430 g (28.3%) of fish waste was degraded. Analyses indicated that the 96 h culture of inoculated fish waste possessed comparable fertilizing ability to commercial fertilizers in hydroponic culture with amino acid contents of 6.91 g 100 g(-1). Therefore, the scaled-up production achieved a more satisfactory fish waste degradation rate (3.61 g h(-1)) than the flask-scale production (0.24 g h(-1)). The biodegraded broth of fish waste at room temperature did not undergo putrefaction for 6 months due to the addition of 1% lactate.

  1. blend4php: a PHP API for galaxy.

    Science.gov (United States)

    Wytko, Connor; Soto, Brian; Ficklin, Stephen P

    2017-01-01

    Galaxy is a popular framework for execution of complex analytical pipelines typically for large data sets, and is a commonly used for (but not limited to) genomic, genetic and related biological analysis. It provides a web front-end and integrates with high performance computing resources. Here we report the development of the blend4php library that wraps Galaxy's RESTful API into a PHP-based library. PHP-based web applications can use blend4php to automate execution, monitoring and management of a remote Galaxy server, including its users, workflows, jobs and more. The blend4php library was specifically developed for the integration of Galaxy with Tripal, the open-source toolkit for the creation of online genomic and genetic web sites. However, it was designed as an independent library for use by any application, and is freely available under version 3 of the GNU Lesser General Public License (LPGL v3.0) at https://github.com/galaxyproject/blend4phpDatabase URL: https://github.com/galaxyproject/blend4php.

  2. When API Mass Spectrometry Meets Super Atmospheric Pressure Ion Sources.

    Science.gov (United States)

    Chen, Lee Chuin

    2015-01-01

    In a tutorial paper on the application of free-jet technique for API-MS, John Fenn mentioned that "…for a number of years and a number of reasons, it has been found advantageous in many situations to carry out the ionization process in gas at pressures up to 1000 Torr or more" (Int. J. Mass Spectrom. 200: 459-478, 2000). In fact, the first ESI mass spectrometer constructed by Yamashita and Fenn had a counter-flow curtain gas source at 1050 Torr (ca. 1.4 atm) to sweep away the neutral (J. Phys. Chem. 88: 4451-4459, 1984). For gaseous ionization using electrospray plume, theoretical analysis also shows that "super-atmospheric operation would be more preferable in space-charge-limited situations."(Int. J. Mass Spectrom. 300: 182-193, 2011). However, electrospray and the corona-based chemical ion source (APCI) in most commercial instrument are basically operated under an atmospheric pressure ambient, perhaps out of the concern of safety, convenience and simplicity in maintenance. Running the ion source at pressure much higher than 1 atm is not so common, but had been done by a number of groups as well as in our laboratory. A brief review on these ion sources will be given in this paper.

  3. Mating flights select for symmetry in honeybee drones (Apis mellifera).

    Science.gov (United States)

    Jaffé, Rodolfo; Moritz, Robin F A

    2010-03-01

    Males of the honeybee (Apis mellifera) fly to specific drone congregation areas (DCAs), which virgin queens visit in order to mate. From the thousands of drones that are reared in a single colony, only very few succeed in copulating with a queen, and therefore, a strong selection is expected to act on adult drones during their mating flights. In consequence, the gathering of drones at DCAs may serve as an indirect mate selection mechanism, assuring that queens only mate with those individuals having a better flight ability and a higher responsiveness to the queen's visual and chemical cues. Here, we tested this idea relying on wing fluctuating asymmetry (FA) as a measure of phenotypic quality. By recapturing marked drones at a natural DCA and comparing their size and FA with a control sample of drones collected at their maternal hives, we were able to detect any selection on wing size and wing FA occurring during the mating flights. Although we found no solid evidence for selection on wing size, wing FA was found to be significantly lower in the drones collected at the DCA than in those collected at the hives. Our results demonstrate the action of selection during drone mating flights for the first time, showing that developmental stability can influence the mating ability of honeybee drones. We therefore conclude that selection during honeybee drone mating flights may confer some fitness advantages to the queens.

  4. Energetic feedings influence beeswax production by Apis mellifera L. honeybees

    Directory of Open Access Journals (Sweden)

    Marcela Pedraza Carrillo

    2015-02-01

    Full Text Available The effect of different types of energy feeding (sugar syrup, inverted sugar and juice of sugar-cane on beeswax production and its economic feasibility are evaluated. Twenty beehives of Africanized Apis mellifera were selected, and five were used for each type of feeding. The treatments were T1 (sugar-cane juice, T2 (sugar syrup and T3 (inverted sugar. Feedings was provided by Boardman feeders and the amount was adjusted according to consumption. A layer of beeswax was manually set up into the honeybee nest and beeswax built area was measured weekly. Total reducing sugar, calorimetry, dry matter and ashes of all feedings were analyzed. Data were analyzed by analysis of variance with Tukey’s test to determine differences among averages. The average consumption of inverted sugar was significantly lower than that of other treatments. The highest beeswax production average occurred in the sugar syrup treatment. The highest average of ashes, dry matter and reducing sugar occurred, respectively, in sugar-cane juice, inverted sugar and sugar syrup. Sugar syrup may be an alternative energy source for beeswax production, although sugar-cane juice may be more profitable.

  5. Web API for biology with a workflow navigation system.

    Science.gov (United States)

    Kwon, Yeondae; Shigemoto, Yasumasa; Kuwana, Yoshikazu; Sugawara, Hideaki

    2009-07-01

    DNA Data Bank of Japan (DDBJ) provides Web-based systems for biological analysis, called Web APIs for biology (WABI). So far, we have developed over 20 SOAP services and several workflows that consist of a series of method invocations. In this article, we present newly developed services of WABI, that is, REST-based Web services, additional workflows and a workflow navigation system. Each Web service and workflow can be used as a complete service or a building block for programmers to construct more complex information processing systems. The workflow navigation system aims to help non-programming biologists perform analysis tasks by providing next applicable services on Web browsers according to the output of a previously selected service. With this function, users can apply multiple services consecutively only by following links without any programming or manual copy-and-paste operations on Web browsers. The listed services are determined automatically by the system referring to the dictionaries of service categories, the input/output types of services and HTML tags. WABI and the workflow navigation system are freely accessible at http://www.xml.nig.ac.jp/index.html and http://cyclamen.ddbj.nig.ac.jp/, respectively.

  6. The Influence of Niobium on SSC Resistance in API Steels

    Energy Technology Data Exchange (ETDEWEB)

    Malina, J.; Malina, M.; Novosel-Radovic, V. [Zagreb Univ., Faculty of Metallurgy (Croatia)

    2004-07-01

    Full text of publication follows: Oil country tubular goods are often exposed to aggressive sour environments in oil wells where H{sub 2}S promotes sulfide stress cracking, SSC. This is one of the most dangerous corrosion phenomena characterized by unpredictable and sudden cracking of steel constructions in service. SSC results in a variety of fracture features having a common relationship to the presence of hydrogen in metal and therefore it belongs to hydrogen embrittlement phenomena, HE. For the production of sulfide stress cracking resistant (SSC) tubing from steels included in Group 3 of API Specification 5CT, in this study two experimental Cr-Mo steels (without niobium and with niobium microalloyed) were tested. The influence of Nb precipitates in pipeline steels on the sensitivity to sulfide stress cracking was examined in laboratory experiments using three point bent beam tests. High mechanical properties were achieved through quenching and tempering procedures. Better corrosion resistance of Nb-containing steel is the consequence of microstructural parameters obtained through strengthening mechanism with microalloying element. Besides the usual mechanical, metallographic, SEM and TEM analyses, as complementary method the X-ray diffraction was used. It was revealed that tetragonality of crystal lattice, dislocation density and residual stresses are in good correlation with Sc-vales from corrosion tests. Nb precipitates (especially niobium carbides) have beneficial influence when producing the steels with low sensitivity to SSC. (authors)

  7. SIP APIs for Voice and Video Communications on the Web

    CERN Document Server

    Davids, Carol; Singh, Kundan; Sinnreich, Henry; Wimmreuter, Wilhelm

    2011-01-01

    Existing standard protocols for the web and Internet telephony fail to deliver real-time interactive communication from within a web browser. In particular, the client-server web protocol over reliable TCP is not always suitable for end-to-end low latency media path needed for interactive voice and video communication. To solve this, we compare the available platform options using the existing technologies such as modifying the web programming language and protocol, using an existing web browser plugin, and a separate host resident application that the web browser can talk to. We argue that using a separate application as an adaptor is a promising short term as well as long-term strategy for voice and video communications on the web. Our project aims at developing the open technology and sample implementations for web-based real-time voice and video communication applications. We describe the architecture of our project including (1) a RESTful web communication API over HTTP inspired by SIP message flows, (2)...

  8. Nosema Tolerant Honeybees (Apis mellifera) Escape Parasitic Manipulation of Apoptosis

    Science.gov (United States)

    Kurze, Christoph; Le Conte, Yves; Dussaubat, Claudia; Erler, Silvio; Kryger, Per; Lewkowski, Oleg; Müller, Thomas; Widder, Miriam; Moritz, Robin F. A.

    2015-01-01

    Apoptosis is not only pivotal for development, but also for pathogen defence in multicellular organisms. Although numerous intracellular pathogens are known to interfere with the host’s apoptotic machinery to overcome this defence, its importance for host-parasite coevolution has been neglected. We conducted three inoculation experiments to investigate in the apoptotic respond during infection with the intracellular gut pathogen Nosema ceranae, which is considered as potential global threat to the honeybee (Apis mellifera) and other bee pollinators, in sensitive and tolerant honeybees. To explore apoptotic processes in the gut epithelium, we visualised apoptotic cells using TUNEL assays and measured the relative expression levels of subset of candidate genes involved in the apoptotic machinery using qPCR. Our results suggest that N. ceranae reduces apoptosis in sensitive honeybees by enhancing inhibitor of apoptosis protein-(iap)-2 gene transcription. Interestingly, this seems not be the case in Nosema tolerant honeybees. We propose that these tolerant honeybees are able to escape the manipulation of apoptosis by N. ceranae, which may have evolved a mechanism to regulate an anti-apoptotic gene as key adaptation for improved host invasion. PMID:26445372

  9. Nosema Tolerant Honeybees (Apis mellifera Escape Parasitic Manipulation of Apoptosis.

    Directory of Open Access Journals (Sweden)

    Christoph Kurze

    Full Text Available Apoptosis is not only pivotal for development, but also for pathogen defence in multicellular organisms. Although numerous intracellular pathogens are known to interfere with the host's apoptotic machinery to overcome this defence, its importance for host-parasite coevolution has been neglected. We conducted three inoculation experiments to investigate in the apoptotic respond during infection with the intracellular gut pathogen Nosema ceranae, which is considered as potential global threat to the honeybee (Apis mellifera and other bee pollinators, in sensitive and tolerant honeybees. To explore apoptotic processes in the gut epithelium, we visualised apoptotic cells using TUNEL assays and measured the relative expression levels of subset of candidate genes involved in the apoptotic machinery using qPCR. Our results suggest that N. ceranae reduces apoptosis in sensitive honeybees by enhancing inhibitor of apoptosis protein-(iap-2 gene transcription. Interestingly, this seems not be the case in Nosema tolerant honeybees. We propose that these tolerant honeybees are able to escape the manipulation of apoptosis by N. ceranae, which may have evolved a mechanism to regulate an anti-apoptotic gene as key adaptation for improved host invasion.

  10. Cytosine modifications in the honey bee (Apis mellifera worker genome

    Directory of Open Access Journals (Sweden)

    Erik Magne Koscielniak Rasmussen

    2015-02-01

    Full Text Available Epigenetic changes enable genomes to respond to changes in the environment, such as altered nutrition, activity, or social setting. Epigenetic modifications, thereby, provides a source of phenotypic plasticity in many species. The honey bee (Apis mellifera uses nutritionally sensitive epigenetic control mechanisms in the development of the royal caste (queens and the workers. The workers are functionally sterile females that can take on a range of distinct physiological and/or behavioral phenotypes in response to environmental changes. Honey bees have a wide repertoire of epigenetic mechanisms which, as in mammals, includes cytosine methylation, hydroxymethylated cytosines, together with the enzymatic machinery responsible for these cytosine modifications. Current data suggests that honey bees provide an excellent system for studying the social repertoire of the epigenome. In this review, we elucidate what is known so far about the honey bee epigenome and its mechanisms. Our discussion includes what may distinguish honey bees from other model animals, how the epigenome can influence worker behavioral task separation, and how future studies can answer central questions about the role of the epigenome in social behavior.

  11. blend4php: a PHP API for galaxy

    Science.gov (United States)

    Wytko, Connor; Soto, Brian; Ficklin, Stephen P.

    2017-01-01

    Galaxy is a popular framework for execution of complex analytical pipelines typically for large data sets, and is a commonly used for (but not limited to) genomic, genetic and related biological analysis. It provides a web front-end and integrates with high performance computing resources. Here we report the development of the blend4php library that wraps Galaxy’s RESTful API into a PHP-based library. PHP-based web applications can use blend4php to automate execution, monitoring and management of a remote Galaxy server, including its users, workflows, jobs and more. The blend4php library was specifically developed for the integration of Galaxy with Tripal, the open-source toolkit for the creation of online genomic and genetic web sites. However, it was designed as an independent library for use by any application, and is freely available under version 3 of the GNU Lesser General Public License (LPGL v3.0) at https://github.com/galaxyproject/blend4php. Database URL: https://github.com/galaxyproject/blend4php PMID:28077564

  12. Activity of telomerase and telomeric length in Apis mellifera.

    Science.gov (United States)

    Korandová, Michala; Frydrychová, Radmila Čapková

    2016-06-01

    Telomerase is an enzyme that adds repeats of DNA sequences to the ends of chromosomes, thereby preventing their shortening. Telomerase activity is associated with proliferative status of cells, organismal development, and aging. We report an analysis of telomerase activity and telomere length in the honeybee, Apis mellifera. Telomerase activity was found to be regulated in a development and caste-specific manner. During the development of somatic tissues of larval drones and workers, telomerase activity declined to 10 % of its level in embryos and remained low during pupal and adult stages but was upregulated in testes of late pupae, where it reached 70 % of the embryo level. Upregulation of telomerase activity was observed in the ovaries of late pupal queens, reaching 160 % of the level in embryos. Compared to workers and drones, queens displayed higher levels of telomerase activity. In the third larval instar of queens, telomerase activity reached the embryo level, and an enormous increase was observed in adult brains of queens, showing a 70-fold increase compared to a brain of an adult worker. Southern hybridization of terminal TTAGG fragments revealed a high variability of telomeric length between different individuals, although the same pattern of hybridization signals was observed in different tissues of each individual.

  13. Mating flights select for symmetry in honeybee drones ( Apis mellifera)

    Science.gov (United States)

    Jaffé, Rodolfo; Moritz, Robin F. A.

    2010-03-01

    Males of the honeybee ( Apis mellifera) fly to specific drone congregation areas (DCAs), which virgin queens visit in order to mate. From the thousands of drones that are reared in a single colony, only very few succeed in copulating with a queen, and therefore, a strong selection is expected to act on adult drones during their mating flights. In consequence, the gathering of drones at DCAs may serve as an indirect mate selection mechanism, assuring that queens only mate with those individuals having a better flight ability and a higher responsiveness to the queen’s visual and chemical cues. Here, we tested this idea relying on wing fluctuating asymmetry (FA) as a measure of phenotypic quality. By recapturing marked drones at a natural DCA and comparing their size and FA with a control sample of drones collected at their maternal hives, we were able to detect any selection on wing size and wing FA occurring during the mating flights. Although we found no solid evidence for selection on wing size, wing FA was found to be significantly lower in the drones collected at the DCA than in those collected at the hives. Our results demonstrate the action of selection during drone mating flights for the first time, showing that developmental stability can influence the mating ability of honeybee drones. We therefore conclude that selection during honeybee drone mating flights may confer some fitness advantages to the queens.

  14. Comparative sucrose responsiveness in Apis mellifera and A. cerana foragers.

    Science.gov (United States)

    Yang, Wenchao; Kuang, Haiou; Wang, Shanshan; Wang, Jie; Liu, Wei; Wu, Zhenhong; Tian, Yuanyuan; Huang, Zachary Y; Miao, Xiaoqing

    2013-01-01

    In the European honey bee, Apis mellifera, pollen foragers have a higher sucrose responsiveness than nectar foragers when tested using a proboscis extension response (PER) assay. In addition, Africanized honey bees have a higher sucrose responsiveness than European honey bees. Based on the biology of the Eastern honey bee, A. cerana, we hypothesized that A. cerana should also have a higher responsiveness to sucrose than A. mellifera. To test this hypothesis, we compared the sucrose thresholds of pollen foragers and nectar foragers in both A. cerana and A. mellifera in Fujian Province, China. Pollen foragers were more responsive to sucrose than nectar foragers in both species, consistent with previous studies. However, contrary to our hypothesis, A. mellifera was more responsive than A. cerana. We also demonstrated that this higher sucrose responsiveness in A. mellifera was not due to differences in the colony environment by co-fostering two species of bees in the same mixed-species colonies. Because A. mellifera foragers were more responsive to sucrose, we predicted that their nectar foragers should bring in less concentrated nectar compared to that of A. cerana. However, we found no differences between the two species. We conclude that A. cerana shows a different pattern in sucrose responsiveness from that of Africanized bees. There may be other mechanisms that enable A. cerana to perform well in areas with sparse nectar resources.

  15. Western honeybee drones and workers (Apis mellifera ligustica) have different olfactory mechanisms than eastern honeybees (Apis cerana cerana).

    Science.gov (United States)

    Woltedji, Dereje; Song, Feifei; Zhang, Lan; Gala, Alemayehu; Han, Bin; Feng, Mao; Fang, Yu; Li, Jianke

    2012-09-07

    The honeybees Apis mellifera ligustica (Aml) and Apis cerana cerana (Acc) are two different western and eastern bee species that evolved in distinct ecologies and developed specific antennal olfactory systems for their survival. Knowledge of how their antennal olfactory systems function in regards to the success of each respective bee species is scarce. We compared the antennal morphology and proteome between respective sexually mature drones and foraging workers of both species using a scanning electron microscope, two-dimensional electrophoresis, mass spectrometry, bioinformatics, and quantitative real-time polymerase chain reaction. Despite the general similarities in antennal morphology of the drone and worker bees between the two species, a total of 106 and 100 proteins altered their expression in the drones' and the workers' antennae, respectively. This suggests that the differences in the olfactory function of each respective bee are supported by the change of their proteome. Of the 106 proteins that altered their expression in the drones, 72 (68%) and 34 (32%) were overexpressed in the drones of Aml and Acc, respectively. The antennae of the Aml drones were built up by the highly expressed proteins that were involved in carbohydrate metabolism and energy production, molecular transporters, antioxidation, and fatty acid metabolism in contrast to the Acc drones. This is believed to enhance the antennal olfactory functions of the Aml drones as compared to the Acc drones during their mating flight. Likewise, of the 100 proteins with expression changes between the worker bees of the two species, 67% were expressed in higher levels in the antennae of Aml worker contrasting to 33% in the Acc worker. The overall higher expressions of proteins related to carbohydrate metabolism and energy production, molecular transporters, and antioxidation in the Aml workers compared with the Acc workers indicate the Aml workers require more antennal proteins for their olfactory

  16. The construction of prokaryotic expression system for human Api6 fusion protein%人Api6融合蛋白原核表达系统的构建

    Institute of Scientific and Technical Information of China (English)

    方严; 练雪梅

    2011-01-01

    Objective:To further study the pathophysiological function of human Api6 fusion protein expression using a prokaryotic expression system. Methods: Api6 cDNA was inserted into ppSUMO vector to form recombinant plasmid pp-SUMO-Api6;recombinant plasmid ppSUMO-Api6 was trans-formed into prokaryotic expression host E.coli Rosetta;prokaryotic expression method was optimized to induce expression of recombi-nant protein Api6 in different conditions of E.coli density,temperature,IPTG concentration and induction time;recombinant protein Api6 was identified by Western blot. Results:The recombinant plasmid ppSUMO-Api6 was successfully constructed;the recombinant protein Api6 was successfully induced,and the expression of recombinant protein ppSUMO-Api6 from the prokaryotic expression system was optimized in the condition of OD600=0.5-0.8,16℃,0.5 mmol/L IPTG and 16 h induction.Conclusion:Recombinant plasmid ppSUMO-Api6 was converted into the prokaryotic expression host E.coli Rosetta to get the recombinant strain ppSUMO-Api6-Rosetta.Recombinant protein ppSUMO-Api6 was expressed using a prokaryotic expression system at the optimal expression conditions.%目的:本研究拟采用蛋白原核表达的方法,诱导表达人Api6(Apoptosis inhibitor 6)融合蛋白,为进一步研究Api6在凋亡抑制、脂质代谢和肿瘤发生中的作用奠定基础.方法:利用PCR技术扩增人Api6基因,将其克隆至原核表达载体ppSUMO,构建重组质粒ppSUMO-Api6;重组质粒转化原核表达宿主菌E.coli Rosetta感受态;采用蛋白原核表达的方法,不同诱导前菌液浓度,不同温度、不同异丙基硫化半乳糖苷(Isopropy 1-β-D-thiogalaotopyrano-side,IPTG)浓度、不同诱导时间,筛选诱导表达Api6重组蛋白的最优条件;Western blot鉴定其表达情况.结果:成功构建重组质粒ppSUMO-Api6;成功诱导Api6重组蛋白的原核表达,表达的最优条件为:OD600=0.5~0.8的重组菌,16℃、0.5 mmol/L IPTG诱导16 h.结论:含有人Api6 c

  17. Comparison of API 20E and invA PCR for identification of Salmonella enterica isolates from swine production units.

    Science.gov (United States)

    Nucera, Daniele M; Maddox, Carol W; Hoien-Dalen, Patricia; Weigel, Ronald M

    2006-09-01

    API 20E and invA PCR were evaluated for the identification of Salmonella enterica isolates from swine farms. API 20E had the highest agreement with other tests at the 99.9% likelihood level. Both tests had 100% sensitivity and 96% specificity compared to 16S rRNA sequencing. Compared to serotyping, both tests had 96% sensitivity; specificity was 86% for API 20E and 79% for invA PCR.

  18. Mutational epitope analysis and cross-reactivity of two isoforms of Api g 1, the major celery allergen.

    Science.gov (United States)

    Wangorsch, Andrea; Ballmer-Weber, Barbara K; Rösch, Paul; Holzhauser, Thomas; Vieths, Stefan

    2007-04-01

    For better understanding the cross-reactivity between the major birch pollen and celery allergens, Bet v 1 and Api g 1, respectively, putative epitope areas and structurally important positions for IgE-binding of the isoforms Api g 1.01 and Api g 1.02 were point mutated. The IgE binding capacities were measured in ELISA, the IgE cross-reactivity between the isoforms, mutants and Bet v 1 investigated by ELISA-inhibition experiments with serum pools from patients with confirmed celery allergy (DBPCFC). Api g 1.01 displayed a clearly higher frequency and capacity of IgE binding than Api g 1.02. In Api g 1.01, substitution of lysine against glutamic acid at amino acid position 44, a key residue of the Bet v 1 "P-loop", increased the IgE-binding properties. Structural instability due to proline insertion at position 111/112 resulted in loss of IgE binding of Api g 1.01, but not of Api g 1.02. Between Api g 1.01 and Api g 1.02 only partial cross-reactivity was seen. The data suggest that the IgE epitopes of the two isoforms are distinct and that in contrast to Api g 1.01, the "P-loop" region plays an important role for IgE binding of celery allergic subjects to Api g 1.02. Understanding and investigation of the molecular mechanisms in celery allergy is an important step to generate hypoallergenic proteins for safe and efficacious immunotherapy of food allergy.

  19. Software Defined GPS API: Development and Implementation of GPS Correlator Architectures Using MATLAB with Focus on SDR Implementations

    Science.gov (United States)

    2014-05-18

    NO. 0704-0188 3. DATES COVERED (From - To) - UU UU UU UU Approved for public release; distribution is unlimited. Software Defined GPS API : Development...and Implementation of GPS Correlator Architectures Using MATLAB with Focus on SDR Implementations The Software Defined GPS API was created with the...ABOVE ADDRESS. Polytechnic University of Puerto Rico 377 Ponce de Leon Ave. Hato Rey, PR 00918 -0000 ABSTRACT Software Defined GPS API : Development and

  20. Windows API funkcijų sekų perėmimo bibliotekų tyrimas Windows API Hooking Libraries Research

    Directory of Open Access Journals (Sweden)

    Lukas Radvilavičius

    2011-08-01

    Full Text Available

    Nagrinėjamos bibliotekos, skirtos Windows API funkcijų sekų perėmimui. Sekų perėmimas gali būti panaudojamas įvairiose srityse: siekiant išsiaiškinti operacinės sistemos veikimo principus, norint atlikti programos derinimą arba pridėti papildomą funkcionalumą prie jau esamo. Straipsnyje apžvelgiamos Windows API ir pateikiamos trečiųjų šalių priemonės funkcijų sekoms  perimti. Aptariamos jų teikiamos galimybės, panaudojimo specifika. Taip pat atlikti testai, siekiant išsiaiškinti, kuri biblioteka su užduotimis susitvarko greičiausiai. Tyrimui pasirinkti du nemokami gaminiai: Microsoft Detours ir EasyHook bibliotekos.

    Straipsnis lietuvių kalba

    The paper describes methods how to apply Windows API hooking with third party libraries and solutions. In this research were used Windows API function SetWindowsHookEx, Detours and EasyHook libraries. Libraries methods, features and advantages were discussed in this paper. The practical part contains libraries tests. In analysis we tested target program start with hooking library and injected function call.

    Article in Lithuanian

  1. Extracardiac conduit in total cavopulmonary connection Conduto extracardíaco na derivação cavopulmonar total

    Directory of Open Access Journals (Sweden)

    Paulo Paredes Paulista

    2003-09-01

    Full Text Available OBJECTIVE: This study evaluates a new technique of an extracardiac conduit in total cavopulmonary connection in complex congenital heart disease. METHODS: Between May 2000 and October 2002, 18 extracardiac conduit surgeries were performed. The patients' weights ranged from 11 to 29 kilograms, the ages ranged from 1 to 12 years old and 10 patients were male. There were eight patients with tricuspid atresia, eight with univentricular heart, and two with unbalanced total atrioventricular septal defect. There were 17 patients who had been submitted to a previous palliative surgery. The surgery was performed at over 32º centigrade without aortic ischemia. Ten PTFE, 4 Hemashield and 4 bovine pericardium tubes were used with diameters ranging from 16 to 22 mm. RESULTS: There were four cases of tube thrombosis and these patients were a reoperation was performed with one death. In one patient a takedown was performed on the 69th postoperative day. There were three deaths, one due to tube thrombosis and two due to low cardiac output. CONCLUSION: Extracardiac conduit is already used for total cavopulmonary connection, but despite encouraging early results, a longer follow-up is necessary to prove its real advantages.OBJETIVO: Avaliar a utilização de conduto extracardíaco na confecção de derivação cavopulmonar total nos diferentes tipos de cardiopatias complexas nas quais está indicado este tipo de correção. MÉTODO: Entre maio de 2000 e janeiro de 2003, foram operados 18 pacientes, 10 do sexo masculino, com idades entre 1 e 12 anos e peso variando de 11 a 29 quilos. O diagnóstico principal foi atresia tricúspide em oito pacientes, conexão atrioventricular univentricular em oito e defeito total do septo atrioventricular desbalanceado em dois. Apenas um paciente foi operado sem cirurgia paliativa prévia. A operação foi realizada com emprego de circulação extracorpórea com temperatura acima de 32ºC orofaríngea e sem isquemia a

  2. Development of API 5 CT grade P110 steel casing; Desenvolvimento de tubos casing API 5 CT grau P110

    Energy Technology Data Exchange (ETDEWEB)

    Kojima, Sergio Seijo; Roza, Juliana Espinossi

    2003-07-01

    This paper has as objective to discuss the results gotten in experiences carried through in industrial scale for the development of pipes class API 5CT P110 (1), with 244 mm of diameter and 12 mm of thickness. The pipes were welded longitudinally by process ERW (electric resistance welding) and submitted to quenching and tempering heat treatments. A C-Mn-Cr alloy with adequate quenching ability was used for the attainment of a martensite microstructure after quenching. The pipes at a temperature of 880 deg C were quenched in water and subsequently tempered at 515 deg C. The integrity of the weld region was evaluated through non destructive tests and flattening tests, Charpy impact tests and fracture toughness tests (CTOD). The pipes achieved the tension properties stipulated in the standard (yield strength bigger limit of 758 Mpa), and also the energy in the Charpy impact test (transversal), considering that the energy value obtained was 55 J (at 0 deg C) for base metal and 44 J in the weld metal. (author)

  3. [Melissopalynology and trophic niche of Apis cerana ceraca and Apis mellifera ligustica in Yunnan Province of Southwest China].

    Science.gov (United States)

    Liu, Yu-Jia; Zhao, Tian-Rui; Zhao, Feng-Yun

    2013-01-01

    In 2010 and 2011, the honey samples of Apis cerana cerana and A. mellifera ligustica were collected from Kunming and Mengzi of Yunnan Province, respectively, aimed to analyze the melissopalynology and tropic niche of the two bee species. The absolute pollen concentration of the honey of A. cerana cerana was 1.55 x 10(4) ind x g(-1), being significantly higher than that (1.01 x 10(4) ind x g(-1)) of A. mellifera ligustica, and the number of nectar plant species collected by A. cerana cerana was 12.9, also significantly higher than that (7.7) collected by A. mellifera ligustica, indicating that A. cerana cerana could utilize more nectar plants, while A. mellifera ligustica had stronger selectivity to the nectar plants. The trophic niche breadth of A. cerana cerana was 0.35, which was significantly higher than that (0.23) of A. mellifera ligustica. The trophic niche overlap index between the two bee species was 0.71, and the interspecific competition index was 0.93, suggesting that the food competition between A. cerana cerana and A. mellifera ligustica was fierce.

  4. Aneurisma ilíaco associado a fístula arteriovenosa Iliac aneurysm associated with arteriovenous fistula

    Directory of Open Access Journals (Sweden)

    Daniel Mendes Pinto

    2007-09-01

    Full Text Available A ruptura dos aneurismas aorto-ilíacos para a veia ilíaca ou veia cava é uma complicação pouco comum. A hipertensão venosa leva a vários sinais e sintomas, o que dificulta o diagnóstico pré-operatório, tais como edema do membro inferior, dispnéia, hematúria, sinais de insuficiência renal ou cardíaca. Sopro abdominal é a chave do diagnóstico clínico, associado à massa pulsátil e dor abdominal. O reconhecimento da fístula arteriovenosa no pré-operatório é importante para o planejamento cirúrgico. Relatamos um caso de aneurisma da artéria ilíaca comum e interna direita associado a fístula para veia ilíaca comum, cursando, inicialmente, com edema do membro inferior direito e dispnéia, o que levou ao diagnóstico incorreto de trombose venosa profunda.Rupture of aortoiliac aneurysms into the iliac vein or vena cava is an uncommon complication. Many signs and symptoms develop as a result of venous hypertension, which makes preoperative diagnosis difficult, such as leg edema, dyspnea, hematuria, signs of renal or cardiac insufficiency. Abdominal bruit, associated with pulsatile mass and abdominal pain, is the key for clinical diagnosis. Preoperative recognition of arteriovenous fistula is important for surgical planning. We report a case of right internal and common iliac artery aneurysm associated with fistula into the common iliac vein. Initial symptoms were right leg edema and dyspnea, which induced to the incorrect diagnosis of deep vein thrombosis.

  5. Marcapasso cardíaco artificial: considerações pré e per-operatórias

    Directory of Open Access Journals (Sweden)

    Ramos Gilson

    2003-01-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O avanço tecnológico da propedêutica médica diagnóstica em cardiologia difundiu grandemente a indicação de marcapasso (MP cardíaco artificial, definitivo ou temporário. Isso fez com que outros especialistas, além dos cardiologistas, se envolvessem ainda mais no manuseio desses aparelhos. Quando pacientes portadores de MP se apresentam para cirurgia, a participação do anestesiologista pode ser decisiva para o sucesso do procedimento. O objetivo da presente revisão é familiarizar o anestesiologista com as principais indicações clínicas e com o funcionamento desses dispositivos, além dos cuidados pré e per-operatório que se deve ter. CONTEÚDO: Foram tratadas a classificação, funcionamento e as principais indicações clínicas para o implante de MP. Da mesma forma, procurou-se elucidar os principais cuidados pré e per-operatórios relativos ao uso de MP para lograr êxito no procedimento indicado. CONCLUSÕES: O principais conhecimentos sobre o funcionamento do MP e suas indicações clínicas devem fazer parte da prática diária do anestesiologista. Assim, o manuseio e a indicação do MP temporário amplia a atuação desses especialistas, além do que pode salvar vidas, inclusive em situações emergenciais dentro do centro cirúrgico. O uso de eletrocautério deveria ser evitado em portadores de MP.

  6. Optimization on Paddy Crops in Central Java (with Solver, SVD on Least Square and ACO (Ant Colony Algorithm))

    Science.gov (United States)

    Parhusip, H. A.; Trihandaru, S.; Susanto, B.; Prasetyo, S. Y. J.; Agus, Y. H.; Simanjuntak, B. H.

    2017-03-01

    Several algorithms and objective functions on paddy crops have been studied to get optimal paddy crops in Central Java based on the data given from Surakarta and Boyolali. The algorithms are linear solver, least square and Ant Colony Algorithms (ACO) to develop optimization procedures on paddy crops modelled with Modified GSTAR (Generalized Space-Time Autoregressive) and nonlinear models where the nonlinear models are quadratic and power functions. The studied data contain paddy crops from Surakarta and Boyolali determining the best period of planting in the year 1992-2012 for Surakarta where 3 periods for planting are known and the optimal amount of paddy crops in Boyolali in the year 2008-2013. Having these analyses may guide the local agriculture government to give a decision on rice sustainability in its region. The best period for planting in Surakarta is observed, i.e. the best period is in September-December based on the data 1992-2012 by considering the planting area, the cropping area, and the paddy crops are the most important factors to be taken into account. As a result, we can refer the paddy crops in this best period (about 60.4 thousand tons per year) as the optimal results in 1992-2012 where the used objective function is quadratic. According to the research, the optimal paddy crops in Boyolali about 280 thousand tons per year where the studied factors are the amount of rainfalls, the harvested area and the paddy crops in 2008-2013. In this case, linear and power functions are studied to be the objective functions. Compared to all studied algorithms, the linear solver is still recommended to be an optimization tool for a local agriculture government to predict paddy crops in future.

  7. Transplante cardíaco no Instituto Dante Pazzanese de Cardiologia: análise da sobrevida

    Directory of Open Access Journals (Sweden)

    ASSEF Marco Aurélio Salles

    2001-01-01

    Full Text Available CASUÍSTICA E MÉTODOS: Foram analisados, retrospectivamente, 80 transplantes cardíacos realizados no Instituto Dante Pazzanese de Cardiologia (IDPC no período de novembro de 1991 a agosto de 2000. Houve predomínio do sexo masculino em 70% dos casos e a idade variou de 7 a 69 anos, com média de 44,8 anos. Doze (15% pacientes se encontravam em prioridade, em uso de drogas inotrópicas endovenosas no momento do transplante. As etiologias determinantes da insuficiência cardíaca congestiva grave foram: miocardiopatia dilatada idiopática em 37,5%, miocardiopatia isquêmica em 33,75%, miocardiopatia chagásica em 17,5% e outras causas em 11,25%. Foram realizados 78 transplantes ortotópicos e 2 heterotópicos. A técnica empregada foi bicaval/bipulmonar em 63,75%, atrial em 27,5%, bicaval/unipulmonar em 6,25% e heterotópico em 2,5%. A mortalidade hospitalar (30 dias foi de 18,75%. RESULTADOS: A sobrevida para o transplante ortotópico em um ano foi de 72,7%, em cinco anos 61,5% e em sete anos 56,4%. A sobrevida após o transplante foi correlacionada com as variáveis idade, causa de óbito e sexo do doador, e pelo transplante ter sido ou não a primeira cirurgia cardíaca do paciente.

  8. 一种基于新型图模型的 API 推荐系统%APISynth:A New Graph-Based API Recommender System

    Institute of Scientific and Technical Information of China (English)

    吕晨; 姜伟; 虎嵩林

    2015-01-01

    对象实例化是软件工程类库复用中一个常见、复杂的问题.即根据已知源类型的对象,用户需要编写代码对目标类型进行实例化.研究人员提出了若干种 API 自动推荐系统辅助用户解决上述问题.然而这些系统不能同时兼顾准确率和查全率,因此在一定程度上影响了系统的实用性.该文提出了一种兼顾准确率和查全率,基于新型图模型的 API 推荐系统———APISynth.在查全率方面,APISynth 使用一个新颖的全局图模型来表达类库中所有的API 依赖关系和 API 历史使用信息.利用新的全局图模型具有的特殊 Tag 元素和可达性质以避免错误的 API 调用.在准确率方面,APISynth 首先将对象实例化问题建模为 Top-K 子图查询问题,然后设计一种新的支持 DAG 形式解的图搜索算法,避免了传统的最短路径图搜索算法导致的查不准问题.实验结果表明,与现有多种方法相比, APISynth 在准确率和查全率两方面均获得了较大提升.%How to perform object instantiation is a common and complex problem in reusing an existing library.In this problem,given the object of source type,users need to write code to instantiate the destination type.Recently,researchers have proposed a variety of related API automatic recommender systems to fulfill this problem.These systems can assist users to solve the above issue in some degree.However,it is very difficult for such systems to achieve both good recall and precision,which affects the usability.This paper presents APISynth,a new graph-based API recommender system.In order to improve the recall,APISynth utilizes a new global graph model to give a better representation for the API dependencies in the relevant class library,and to include API historical usage information in.A special tag element is added to the new graph together with a new reach ability property to avoid false invocation of APIs

  9. Transcriptome analysis of the Asian honey bee Apis cerana cerana.

    Directory of Open Access Journals (Sweden)

    Zi Long Wang

    Full Text Available BACKGROUND: The Eastern hive honey bee, Apis cerana cerana is a native and widely bred honey bee species in China. Molecular biology research about this honey bee species is scarce, and genomic information for A. c. cerana is not currently available. Transcriptome and expression profiling data for this species are therefore important resources needed to better understand the biological mechanisms of A. c. cerana. In this study, we obtained the transcriptome information of A. c. cerana by RNA-sequencing and compared gene expression differences between queens and workers of A. c. cerana by digital gene expression (DGE analysis. RESULTS: Using high-throughput Illumina RNA sequencing we obtained 51,581,510 clean reads corresponding to 4.64 Gb total nucleotides from a single run. These reads were assembled into 46,999 unigenes with a mean length of 676 bp. Based on a sequence similarity search against the five public databases (NR, Swissport, GO, COG, KEGG with a cut-off E-value of 10(-5 using BLASTX, a total of 24,630 unigenes were annotated with gene descriptions, gene ontology terms, or metabolic pathways. Using these transcriptome data as references we analyzed the gene expression differences between the queens and workers of A. c. cerana using a tag-based digital gene expression method. We obtained 5.96 and 5.66 million clean tags from the queen and worker samples, respectively. A total of 414 genes were differentially expressed between them, with 189 up-regulated and 225 down-regulated in queens. CONCLUSIONS: Our transcriptome data provide a comprehensive sequence resource for future A. c. cerana study, establishing an important public information platform for functional genomic studies in A. c. cerana. Furthermore, the DGE data provide comprehensive gene expression information for the queens and workers, which will facilitate our understanding of the molecular mechanisms of the different physiological aspects of the two castes.

  10. Social waves in giant honeybees (Apis dorsata) elicit nest vibrations.

    Science.gov (United States)

    Kastberger, Gerald; Weihmann, Frank; Hoetzl, Thomas

    2013-07-01

    Giant honeybees (Apis dorsata) nest in the open and have developed a wide array of strategies for colony defence, including the Mexican wave-like shimmering behaviour. In this collective response, the colony members perform upward flipping of their abdomens in coordinated cascades across the nest surface. The time-space properties of these emergent waves are response patterns which have become of adaptive significance for repelling enemies in the visual domain. We report for the first time that the mechanical impulse patterns provoked by these social waves and measured by laser Doppler vibrometry generate vibrations at the central comb of the nest at the basic (='natural') frequency of 2.156 ± 0.042 Hz which is more than double the average repetition rate of the driving shimmering waves. Analysis of the Fourier spectra of the comb vibrations under quiescence and arousal conditions provoked by mass flight activity and shimmering waves gives rise to the proposal of two possible models for the compound physical system of the bee nest: According to the elastic oscillatory plate model, the comb vibrations deliver supra-threshold cues preferentially to those colony members positioned close to the comb. The mechanical pendulum model predicts that the comb vibrations are sensed by the members of the bee curtain in general, enabling mechanoreceptive signalling across the nest, also through the comb itself. The findings show that weak and stochastic forces, such as general quiescence or diffuse mass flight activity, cause a harmonic frequency spectrum of the comb, driving the comb as an elastic plate. However, shimmering waves provide sufficiently strong forces to move the nest as a mechanical pendulum. This vibratory behaviour may support the colony-intrinsic information hypothesis herein that the mechanical vibrations of the comb provoked by shimmering do have the potential to facilitate immediate communication of the momentary defensive state of the honeybee nest to

  11. Viruses associated with ovarian degeneration in Apis mellifera L. queens.

    Directory of Open Access Journals (Sweden)

    Laurent Gauthier

    Full Text Available Queen fecundity is a critical issue for the health of honeybee (Apis mellifera L. colonies, as she is the only reproductive female in the colony and responsible for the constant renewal of the worker bee population. Any factor affecting the queen's fecundity will stagnate colony development, increasing its susceptibility to opportunistic pathogens. We discovered a pathology affecting the ovaries, characterized by a yellow discoloration concentrated in the apex of the ovaries resulting from degenerative lesions in the follicles. In extreme cases, marked by intense discoloration, the majority of the ovarioles were affected and these cases were universally associated with egg-laying deficiencies in the queens. Microscopic examination of the degenerated follicles showed extensive paracrystal lattices of 30 nm icosahedral viral particles. A cDNA library from degenerated ovaries contained a high frequency of deformed wing virus (DWV and Varroa destructor virus 1 (VDV-1 sequences, two common and closely related honeybee Iflaviruses. These could also be identified by in situ hybridization in various parts of the ovary. A large-scale survey for 10 distinct honeybee viruses showed that DWV and VDV-1 were by far the most prevalent honeybee viruses in queen populations, with distinctly higher prevalence in mated queens (100% and 67%, respectively for DWV and VDV-1 than in virgin queens (37% and 0%, respectively. Since very high viral titres could be recorded in the ovaries and abdomens of both functional and deficient queens, no significant correlation could be made between viral titre and ovarian degeneration or egg-laying deficiency among the wider population of queens. Although our data suggest that DWV and VDV-1 have a role in extreme cases of ovarian degeneration, infection of the ovaries by these viruses does not necessarily result in ovarian degeneration, even at high titres, and additional factors are likely to be involved in this pathology.

  12. Detection of Illicit Drugs by Trained Honeybees (Apis mellifera.

    Directory of Open Access Journals (Sweden)

    Matthias Schott

    Full Text Available Illegal drugs exacerbate global social challenges such as substance addiction, mental health issues and violent crime. Police and customs officials often rely on specially-trained sniffer dogs, which act as sensitive biological detectors to find concealed illegal drugs. However, the dog "alert" is no longer sufficient evidence to allow a search without a warrant or additional probable cause because cannabis has been legalized in two US states and is decriminalized in many others. Retraining dogs to recognize a narrower spectrum of drugs is difficult and training new dogs is time consuming, yet there are no analytical devices with the portability and sensitivity necessary to detect substance-specific chemical signatures. This means there is currently no substitute for sniffer dogs. Here we describe an insect screening procedure showing that the western honeybee (Apis mellifera can sense volatiles associated with pure samples of heroin and cocaine. We developed a portable electroantennographic device for the on-site measurement of volatile perception by these insects, and found a positive correlation between honeybee antennal responses and the concentration of specific drugs in test samples. Furthermore, we tested the ability of honeybees to learn the scent of heroin and trained them to show a reliable behavioral response in the presence of a highly-diluted scent of pure heroin. Trained honeybees could therefore be used to complement or replace the role of sniffer dogs as part of an automated drug detection system. Insects are highly sensitive to volatile compounds and provide an untapped resource for the development of biosensors. Automated conditioning as presented in this study could be developed as a platform for the practical detection of illicit drugs using insect-based sensors.

  13. Acaricide, fungicide and drug interactions in honey bees (Apis mellifera.

    Directory of Open Access Journals (Sweden)

    Reed M Johnson

    Full Text Available BACKGROUND: Chemical analysis shows that honey bees (Apis mellifera and hive products contain many pesticides derived from various sources. The most abundant pesticides are acaricides applied by beekeepers to control Varroa destructor. Beekeepers also apply antimicrobial drugs to control bacterial and microsporidial diseases. Fungicides may enter the hive when applied to nearby flowering crops. Acaricides, antimicrobial drugs and fungicides are not highly toxic to bees alone, but in combination there is potential for heightened toxicity due to interactive effects. METHODOLOGY/PRINCIPAL FINDINGS: Laboratory bioassays based on mortality rates in adult worker bees demonstrated interactive effects among acaricides, as well as between acaricides and antimicrobial drugs and between acaricides and fungicides. Toxicity of the acaricide tau-fluvalinate increased in combination with other acaricides and most other compounds tested (15 of 17 while amitraz toxicity was mostly unchanged (1 of 15. The sterol biosynthesis inhibiting (SBI fungicide prochloraz elevated the toxicity of the acaricides tau-fluvalinate, coumaphos and fenpyroximate, likely through inhibition of detoxicative cytochrome P450 monooxygenase activity. Four other SBI fungicides increased the toxicity of tau-fluvalinate in a dose-dependent manner, although possible evidence of P450 induction was observed at the lowest fungicide doses. Non-transitive interactions between some acaricides were observed. Sublethal amitraz pre-treatment increased the toxicity of the three P450-detoxified acaricides, but amitraz toxicity was not changed by sublethal treatment with the same three acaricides. A two-fold change in the toxicity of tau-fluvalinate was observed between years, suggesting a possible change in the genetic composition of the bees tested. CONCLUSIONS/SIGNIFICANCE: Interactions with acaricides in honey bees are similar to drug interactions in other animals in that P450-mediated detoxication

  14. Neutralization of Apis mellifera bee venom activities by suramin.

    Science.gov (United States)

    El-Kik, Camila Z; Fernandes, Fabrício F A; Tomaz, Marcelo Amorim; Gaban, Glauco A; Fonseca, Tatiane F; Calil-Elias, Sabrina; Oliveira, Suellen D S; Silva, Claudia L M; Martinez, Ana Maria Blanco; Melo, Paulo A

    2013-06-01

    In this work we evaluated the ability of suramin, a polysulfonated naphthylurea derivative, to antagonize the cytotoxic and enzymatic effects of the crude venom of Apis mellifera. Suramin was efficient to decrease the lethality in a dose-dependent way. The hemoconcentration caused by lethal dose injection of bee venom was abolished by suramin (30 μg/g). The edematogenic activity of the venom (0.3 μg/g) was antagonized by suramin (10 μg/g) in all treatment protocols. The changes in the vascular permeability caused by A. mellifera (1 μg/g) venom were inhibited by suramin (30 μg/g) in the pre- and posttreatment as well as when the venom was preincubated with suramin. In addition, suramin also inhibited cultured endothelial cell lesion, as well as in vitro myotoxicity, evaluated in mouse extensor digitorum longus muscle, which was inhibited by suramin (10 and 25 μM), decreasing the rate of CK release, showing that suramin protected the sarcolemma against damage induced by components of bee venom (2.5 μg/mL). Moreover, suramin inhibited the in vivo myotoxicity induced by i.m. injection of A. mellifera venom in mice (0.5 μg/g). The analysis of the area under the plasma CK vs. time curve showed that preincubation, pre- and posttreatment with suramin (30 μg/g) inhibited bee venom myotoxic activity in mice by about 89%, 45% and 40%, respectively. Suramin markedly inhibited the PLA2 activity in a concentration-dependent way (1-30 μM). Being suramin a polyanion molecule, the effects observed may be due to the interaction of its charges with the polycation components present in A. mellifera bee venom.

  15. An Effective Method for Protecting Native API Hook Attacks in User-mode

    Directory of Open Access Journals (Sweden)

    K. Muthumanickam

    2015-01-01

    Full Text Available Today, many modern malware developers is taking the advantage of Application Programming Interface (API hook technique to take the control of the victim computer which making it difficult to detect their presence. Because of the sophistication of rootkit tools, a remote attacker can use native API to compromise any computer which can later be used for many illegal activities such as sniffing network lines, capturing passwords, sending spam and DDoS attack, etc. Thus to protect end-system by identifying and preventing native API malicious code hooking is a challenging problem to the defenders. Today, many different malware-analysis tools incur specific features against malwares but manual and error-prone. In this study, we proposed a behavior-based monitoring detection system to effectively deal native API hooks in user-mode. Unlike other malware identification techniques, our approach involved dynamically analyzing the behavior of native API call hooking malwares. Comparing our experimental evaluation results with existing tools show better performance with no false positive.

  16. Transplante cardíaco e neoplasias: experiência na Escola Paulista de Medicina da Universidade Federal de São Paulo

    Directory of Open Access Journals (Sweden)

    Mello Junior Walter Teixeira de

    2006-01-01

    Full Text Available OBJETIVOS: Analisar ocorrência e tipos de neoplasias que se desenvolveram em pacientes submetidos a transplante cardíaco ortotópico, no Programa de Transplante Cardíaco da Escola de Paulista de Medicina da Universidade Federal de São Paulo. MÉTODOS: O presente estudo apresenta uma análise observacional de 106 pacientes submetidos a transplante cardíaco ortotópico, no período de novembro de 1986 a setembro de 2002, que sobreviveram por período superior a trinta dias após o procedimento. O regime de imunossupressão consistiu de terapia tríplice com ciclosporina A, azatioprina e corticosteróide. Apenas dois pacientes receberam, além da terapia tríplice, a adição de ortoclone OKT-3. O período médio de acompanhamento foi de 61,4 meses. (variação de dois meses a 192 meses. RESULTADOS: Vinte e três pacientes (21,3% desenvolveram neoplasias, dos quais 56,5% apresentaram neoplasia de pele, 30,1% apresentaram tumores sólidos e 13,4%, doença linfoproliferativa pós-transplante (DLPT. O intervalo médio entre o transplante e o diagnóstico de neoplasia foi: pele - 54,9 meses, tumores sólidos - 24,8 meses e DLPT - 70,3 meses. CONCLUSÕES: A ocorrência de neoplasias malignas foi relativamente comum na população analisada. O câncer de pele prevaleceu em relação às demais neoplasias e os tumores sólidos foram mais diagnosticados do que as doenças linfoproliferativas nessa série de pacientes.

  17. Un sistema presión-volumen para la medición de propiedades mecánicas de vasos cardíacos menores

    OpenAIRE

    2013-01-01

    Las propiedades mecánicas de vasos cardíacos menores (VM) son poco estudiadas. El sistema Presión-Volumen (PV) propuesto, desarrollado para evaluar comportamientos esfuerzo-deformación (σ-ε) de VM, permite variar controladamente la presión interna y simular alteraciones geométricas al tubo. Se obtuvieron imágenes de las geometrías para cada valor de presión interna. Asumiendo el tubo en equilibrio, con pared elástica y presiones conocidas, se determinaron esfuerzo y deformación circunferencia...

  18. Acometimento cardíaco em pacientes com doença de Chagas aguda em microepidemia familiar, em Abaetetuba, na Amazônia Brasileira

    OpenAIRE

    Pinto, Ana Yecê das Neves; Harada,Geraldo Saburo; Valente,Vera da Costa; Abud,José Elson Araújo; Gomes,Francisco dos Santos; Souza,Gilberto César Rodrigues de; Valente,Sebastião Aldo da Silva

    2001-01-01

    Os autores mostram os principais achados clínicos relativos ao acometimento cardíaco, em pacientes portadores de doença de Chagas aguda em mais um episódio de microepidemia familiar na Amazônia brasileira. Foram estudados 13 pacientes com doença de Chagas aguda, procedentes do município de Abaetetuba-PA e submetidos à avaliação clínica e cardiológica, eletrocardiograma e ecocardiograma. As extra-sístoles supraventriculares e/ou ventriculares ocorreram em 38,5% dos casos. Bloqueios de ramo dir...

  19. Mixoma cardíaco con diagnóstico prenatal. Presentación de un caso y revisión de literatura.

    OpenAIRE

    Ríos, Josías; Médico residente del Servicio de Cirugía Cardiovascular y Tórax. Instituto Nacional Cardiovascular (INCOR), EsSalud. Lima.; Chávarri, Fernando; Médico asistente del Departamento de Cardiopediatría. Instituto Nacional Cardiovascular (INCOR), EsSalud. Lima,; Morales, Gabriela; Médico asistente del Departamento de Cardiopediatría. Instituto Nacional Cardiovascular (INCOR), EsSalud. Lima,; Vera, Luis; Médico asistente del Departamento de Cardiopediatría. Instituto Nacional Cardiovascular (INCOR), EsSalud. Lima,; Zapatel, Carlos; Médico residente del Servicio de Cirugía Cardiovascular y Tórax. Hospital Hipólito Unanue. Lima.; Adrianzén, Manuel; Médico asistente del Departamento de Cardiopediatría. Instituto Nacional Cardiovascular (INCOR), EsSalud. Lima.

    2012-01-01

    El mixoma es la tumoración cardíaca más frecuente en el adulto, constituyendo aproximadamente el 50% de las mismas. En la edad pediátrica el tumor cardíaco más frecuente es el rabdomioma, generalmente asociado a esclerosis tuberosa. La presentación del mixoma en pacientes pediátricos es muy rara y más aun en la etapa neonatal. Presentamos el caso de una paciente de sexo femenino que fue sometida a cirugía a los 8 días de nacida por presentar dos tumoraciones intracardiacas diagnosticadas en l...

  20. Taponamiento cardíaco debido a perforación de la aurícula derecha por catéter venoso central

    OpenAIRE

    V. Ramos Medina; I. Palomo Gómez; P.J. Aranda Granados; J.L. Palomo Rando; M. Salguero Villadiego

    2014-01-01

    El taponamiento cardíaco es una complicación devastadora de los catéteres venosos centrales. Se debe en general a que la punta del catéter alcanza indebidamente las cavidades cardíacas derechas. Para la correcta identificación en la autopsia por el patólogo forense es importante que a los pacientes fallecidos en los hospitales que van a ser sometidos a autopsia no se les retiren los dispositivos que tengan insertados, e igualmente que no se les retiren en la sala de autopsias hasta que no se ...

  1. Taponamiento cardíaco secundario a carcinoma papilar esclerosante difuso de tiroides Metastatic cardiac tamponade as initial manifestation of papillary thyroid carcinoma

    OpenAIRE

    2011-01-01

    El carcinoma papilar, variante esclerosante difusa, corresponde al 2% de todos los carcinomas papilares de la tiroides. Se caracteriza por comprometer de manera difusa y bilateral a la glándula tiroides. Clínicamente se manifiesta con metástasis ganglionares y pulmonares, afectando predominantemente a mujeres jóvenes. Se describe un caso de taponamiento cardíaco como presentación inicial de un carcinoma papilar de tiroides variante esclerosante difusa. Una mujer de 32 años concurrió al servic...

  2. Analgesia, sedação e relaxamento neuromuscular no doente ventilado em cuidados intensivos cardíacos: parte III: relaxantes neuromusculares

    OpenAIRE

    Vilela, H; D. Ferreira

    2006-01-01

    Neste artigo são revistos aspectos clínicos relevantes relacionados com a sedação, analgesia e relaxamento neuromuscular em cuidados intensivos cardíacos, incluindo métodos de monitorização e opções terapêuticas disponíveis. São ainda abordadas as implicações fisiopatológicas da dor, agitação, ansiedade e delírio no doente ventilado. Apesar de terem sido publicadas recentemente normas de orientação para sedação, analgesia e relaxamento neuromuscular em Cuidados I...

  3. Analgesia, sedação e relaxamento neuromuscular no doente ventilado em cuidados intensivos cardíacos: parte II: sedação

    OpenAIRE

    Vilela, H; D. Ferreira

    2006-01-01

    Neste artigo são revistos aspectos clínicos relevantes relacionados com a sedação, analgesia e relaxamento neuromuscular em cuidados intensivos cardíacos, incluindo métodos de monitorização e opções terapêuticas disponíveis. São ainda abordadas as implicações fisiopatológicas da dor, agitação, ansiedade e delírio no doente ventilado. Apesar de terem sido publicadas recentemente normas de orientação para sedação, analgesia e relaxamento neuromuscular em Cuidados Intensivos, existe ...

  4. Analgesia, sedação e relaxamento neuromuscular no doente ventilado em cuidados intensivos cardíacos: parte I: analgesia

    OpenAIRE

    Vilela, H; D. Ferreira

    2006-01-01

    Neste artigo são revistos aspectos clínicos relevantes relacionados com a sedação, analgesia e relaxamento neuromuscular em Cuidados Intensivos Cardíacos, incluindo métodos de monitorização e opções terapêuticas disponíveis. São ainda abordadas as implicações fisiopatológicas da dor, agitação, ansiedade e delírio no doente ventilado. Apesar de terem sido publicadas recentemente normas de orientação para sedação, analgesia e relaxamento neuromuscular em Cuidados Intensi...

  5. Produtos naturais no comportamento defensivo de Apis mellifera L. = Natural products in the defensive behaviour of Apis mellifera L.

    Directory of Open Access Journals (Sweden)

    Renata Leonardo Lomele

    2010-07-01

    Full Text Available Os objetivos do estudo foram investigar a influência de produtos naturais como capim-limão (Cymbopogon citratus, folhas de abacateiro (Persea americana, casca de café (Coffea arabica e sementes de mamona (Ricinus communis na defensividade de Apis mellifera, e avaliar o efeito destes produtos no desenvolvimento populacional da colmeia. O comportamento defensivo foi avaliado por meio do tempo da primeira ferroada (TPF, número de ferrões (NFB e, o desenvolvimento populacional, pela área de cria aberta e fechada. Observou-se que o tratamento fumaça + sete sementes de mamona apresentou aumento significativo no TPF, em relação ao tratamento sem e com fumaça de maravalha. Com relação ao NFB, verificou-se que os tratamentos fumaça de maravalha + sete sementes de mamona e fumaça de maravalha + 20% de folhas de café foram diferentes do tratamento sem e com fumaça. Os demais tratamentos não diferiram significativamente em relação ao uso da fumaça ou sua ausência. A casca de café e a semente de mamona nãointerferiram no desenvolvimento populacional, sugerindo que estes compostos não foram tóxicos. Pode-se concluir que o uso de sementes de mamona e casca de café na fumaça pode representar importante ferramenta para a redução da defensividade, sem promover toxicidade para A. mellifera.The goal was to investigate the influence of natural products such as lemongrass (Cymbopogon citratus, dried avocado leaves (Persea americana, coffee husk (Coffea arabica and castor bean (Ricinus communis in the defense of Apis mellifera, as well the effect of these products on the population development of the beehive. Defensive behavior was evaluated by time of first sting (TFS and number of stingers (NS, and population development, by open brood area and operculated brood. It was observed that the treatment with smoke + seven castor beans presented significant increase in the TFS, for treatment without and with smoke. Regarding NS, it was verified

  6. [New Approach to the Mitotype Classification in Black Honeybee Apis mellifera mellifera and Iberian Honeybee Apis mellifera iberiensis].

    Science.gov (United States)

    Ilyasov, R A; Poskryakov, A V; Petukhov, A V; Nikolenko, A G

    2016-03-01

    The black honeybee Apis mellifera mellifera L. is today the only subspecies of honeybee which is suitable for commercial breeding in the climatic conditions of Northern Europe with long cold winters. The main problem of the black honeybee in Russia and European countries is the preservation of the indigenous gene pool purity, which is lost as a result of hybridization with subspecies, A. m. caucasica, A. m. carnica, A. m. carpatica, and A. m. armeniaca, introduced from southern regions. Genetic identification of the subspecies will reduce the extent of hybridization and provide the gene pool conservation of the black honeybee. Modern classification of the honeybee mitotypes is mainly based on the combined use ofthe DraI restriction endonuclease recognition site polymorphism and sequence polymorphism of the mtDNA COI-COII region. We performed a comparative analysis of the mtDNA COI-COII region sequence polymorphism in the honeybees ofthe evolutionary lineage M from Ural and West European populations of black honeybee A. m. mellifera and Spanish bee A. m. iberiensis. A new approach to the classification of the honeybee M mitotypes was suggested. Using this approach and on the basis of the seven most informative SNPs of the mtDNA COI-COII region, eight honeybee mitotype groups were identified. In addition, it is suggested that this approach will simplify the previously proposed complicated mitotype classification and will make it possible to assess the level of the mitotype diversity and to identify the mitotypes that are the most valuable for the honeybee breeding and rearing.

  7. Effect of chemical composition and welding parameters on microstructure and hardness of API 5I X60 steel weld metals; Efeito da composicao quimica e dos parametros de soldagem sobre a microestrutura e dureza de metais de solda de acos API 5I X60

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Kleber Eduardo Siqueira; Maciel, Theophilo Moura; Albuquerque, Maria Clea Soares de; Almeida, Daisy Martins de [Universidade Federal de Campina Grande, PB (Brazil). Dept. de Engenharia de Materiais

    2004-12-15

    In this work the influence of the variation of the welding parameters and chemical composition of weld metal obtained with different filler metals and b different welding process on the microhardness values and on the acicular ferrite (AF) and primary ferrite (PF) percentiles from the columnar grain region were evaluated. Thee welding processes used were Shielded Manual Metal Arc Welding (SMAW) and semiautomatic processes, such as Flux Cored Arc Welding (FCAW), with protection of CO{sub 2} and inner shield, process MGAW with protection of CO{sub 2} and of CO{sub 2} + Argon and process MGAW with protection of argon. The obtained results indicated that the percentile of AF and PF vary from 48 to 61 per cent and from 28 to 44 per cent respectively. The weld metals that obtained the higher values of A F were those welded by process FCAW inner shield and MGAW with higher values of Equivalent Carbon and with lower heat input. (author)

  8. A Comparison of Article Search APIs via Blinded Experiment and Developer Review

    Directory of Open Access Journals (Sweden)

    Jonathan Rochkind

    2013-01-01

    Full Text Available This study looks at perceived user preference between products that can provide a scholarly article search service via an application programming interface (API. The study set up a blinded review and asked users at Johns Hopkins to select the service that provided the most useful results. Few statistically significant preferences were detected, and some interpretation is provided of what the results might tell us. The specific products evaluated for this study are: Serials Solutions Summon, Ex Libris Primo, EBSCO EDS, EBSCOHost ‘traditional’ API, and Elsevier Scopus. Re-usable open source tools for implementing article search were created to support the study and future development, and a developer review of the APIs is included based on the developer's experience in this implementation.

  9. Studi Eksperimen Distribusi Temperatur Nyala Api Kompor Bioetanol Tipe Side Burner dengan Variasi Diameter Firewall

    Directory of Open Access Journals (Sweden)

    R.R. Vienna Sona Saputri Soetadi

    2012-09-01

    Full Text Available Untuk mendapatkan kompor bioetanol efisiensi thermal maksimal diperlukan penelitian komprehensif. Salah satunya adalah penelitian terhadap posisi peletakkan beban pada kompor bioetanol kompak. Pengujian dilakukan pada kompor uji bioetanol dengan kadar 99%, yaitu kompor bioetanol tipe side burner dengan firewall 2.5 inci dan firewall 3 inci. Pengukuran temperatur api dengan 13 thermocouple K dengan pengukuran searah api keatas setiap 5 mm-an. Kemudian, water boiling test dilakukan untuk mendapatkan daya dan beban dan dilanjutkan mengukur waktu pendidihan air. Hasil penelitian ini menunjukkan gambaran total distribusi temperatur nyala api difusi. Hasil menunjukkan untuk kompor 2.5 inci dengan daya 1.6 kW mempunyai temperatur 542 ºC dengan jarak ketinggian 5 mm dari rim kompor sedangkan kompor 3 inci menghasilkan daya 2.38 kW dengan temperatur 516 ºC.

  10. API2-MALT1 oncoprotein promotes lymphomagenesis via unique program of substrate ubiquitination and proteolysis.

    Science.gov (United States)

    Rosebeck, Shaun; Lim, Megan S; Elenitoba-Johnson, Kojo S J; McAllister-Lucas, Linda M; Lucas, Peter C

    2016-02-26

    Lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) is the most common extranodal B cell tumor and accounts for 8% of non-Hodgkin's lymphomas. Gastric MALT lymphoma is the best-studied example and is a prototypical neoplasm that occurs in the setting of chronic inflammation brought on by persistent infection or autoimmune disease. Cytogenetic abnormalities are commonly acquired during the course of disease and the most common is chromosomal translocation t(11;18)(q21;q21), which creates the API2-MALT1 fusion oncoprotein. t(11;18)-positive lymphomas can be clinically aggressive and have a higher rate of dissemination than t(11;18)-negative tumors. Many cancers, including MALT lymphomas, characteristically exhibit deregulated over-activation of cellular survival pathways, such as the nuclear factor-κB (NF-κB) pathway. Molecular characterization of API2-MALT1 has revealed it to be a potent activator of NF-κB, which is required for API2-MALT1-induced cellular transformation, however the mechanisms by which API2-MALT1 exerts these effects are only recently becoming apparent. The API2 moiety of the fusion binds tumor necrosis factor (TNF) receptor associated factor (TRAF) 2 and receptor interacting protein 1 (RIP1), two proteins essential for TNF receptor-induced NF-κB activation. By effectively mimicking ligand-bound TNF receptor, API2-MALT1 promotes TRAF2-dependent ubiquitination of RIP1, which then acts as a scaffold for nucleating and activating the canonical NF-κB machinery. Activation occurs, in part, through MALT1 moiety-dependent recruitment of TRAF6, which can directly modify NF-κB essential modulator, the principal downstream regulator of NF-κB. While the intrinsic MALT1 protease catalytic activity is dispensable for this canonical NF-κB signaling, it is critical for non-canonical NF-κB activation. In this regard, API2-MALT1 recognizes NF-κB inducing kinase (NIK), the essential upstream regulator of non-canonical NF-κB, and cleaves it to

  11. The SEMAINE API: Towards a Standards-Based Framework for Building Emotion-Oriented Systems

    Directory of Open Access Journals (Sweden)

    Marc Schröder

    2010-01-01

    Full Text Available This paper presents the SEMAINE API, an open source framework for building emotion-oriented systems. By encouraging and simplifying the use of standard representation formats, the framework aims to contribute to interoperability and reuse of system components in the research community. By providing a Java and C++ wrapper around a message-oriented middleware, the API makes it easy to integrate components running on different operating systems and written in different programming languages. The SEMAINE system 1.0 is presented as an example of a full-scale system built on top of the SEMAINE API. Three small example systems are described in detail to illustrate how integration between existing and new components is realised with minimal effort.

  12. Using the RxNorm web services API for quality assurance purposes.

    Science.gov (United States)

    Peters, Lee; Bodenreider, Olivier

    2008-11-06

    Auditing large, rapidly evolving terminological systems is still a challenge. In the case of RxNorm, a standardized nomenclature for clinical drugs, we argue that quality assurance processes can benefit from the recently released application programming interface (API) provided by RxNav. We demonstrate the usefulness of the API by performing a systematic comparison of alternative paths in the RxNorm graph, over several thousands of drug entities. This study revealed potential errors in RxNorm, currently under review. The results also prompted us to modify the implementation of RxNav to navigate the RxNorm graph more accurately. The RxNav web services API used in this experiment is robust and fast.

  13. An API for Integrating Spatial Context Models with Spatial Reasoning Algorithms

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun

    2006-01-01

    The integration of context-aware applications with spatial context models is often done using a common query language. However, algorithms that estimate and reason about spatial context information can benefit from a tighter integration. An object-oriented API makes such integration possible and ...... modeling. The utility of the API is evaluated in several real-world cases from an indoor location system, and spans several types of spatial reasoning algorithms.......The integration of context-aware applications with spatial context models is often done using a common query language. However, algorithms that estimate and reason about spatial context information can benefit from a tighter integration. An object-oriented API makes such integration possible...

  14. APIS : a value-added database of HST UV planetary auroral observations acquired since 1997

    Science.gov (United States)

    Lamy, L.; Henry, F.; Prangé, R.; Le Sidaner, P.

    2014-04-01

    The APIS service http://lesia.obspm.fr/apis/ (Auroral Planetary Imaging and Spectroscopy), aimed at facilitating the use of planetary auroral observations, was presented at EPSC last year, following its opening to the community in July 2013. This facility consists of : - a high level database derived from public Far-UV observations of Jupiter, Io, Ganymede, Saturn, Titan and Uranus acquired by the Hubble Space Telescope since 1997 (36 observational campaigns so far) ; - a specific search interface (Figure 1), aimed at browsing the database freely, quickly and efficiently through relevant search criteria (as planetary longitudes, moon or spacecraft ephemeris etc.). - Virtual-Observatory tools which enable the user to interactively work with images and spectra online. We will present the updated capabilities of APIS and illustrate them with several examples. Several tutorials are also directly available online.

  15. API2-MALT1 oncoprotein promotes lymphomagenesis via unique program of substrate ubiquitination and proteolysis

    Institute of Scientific and Technical Information of China (English)

    Shaun Rosebeck; Megan S Lim; Kojo SJ Elenitoba-Johnson; Linda MMcAllister-Lucas; Peter C Lucas

    2016-01-01

    Lymphoma of mucosa-associated lymphoid tissue(MALT lymphoma) is the most common extranodal B cell tumor and accounts for 8% of non-Hodgkin’s lymphomas. Gastric MALT lymphoma is the best-studied example and is a prototypical neoplasm that occurs in the setting of chronic inflammation brought on by persistent infection or autoimmune disease. Cytogenetic abnormalities are commonly acquired during the course of disease and the most common is chromosomal translocation t(11;18)(q21;q21), which creates the API2-MALT1 fusion oncoprotein. t(11;18)-positive lymphomas can be clinically aggressive and have a higher rate of dissemination than t(11;18)-negative tumors. Many cancers, including MALT lymphomas, characteristically exhibit deregulated over-activation of cellular survival pathways, such as the nuclear factor-κB(NF-κB) pathway. Molecular characterization of API2-MALT1 has revealed it to be a potent activator of NF-κB, which is required for API2-MALT1-induced cellular transformation, however the mechanisms by which API2-MALT1 exerts these effects are only recently becoming apparent. The API2 moiety of the fusion binds tumor necrosis factor(TNF) receptor associated factor(TRAF) 2 and receptor interacting protein 1(RIP1), two proteins essential for TNF receptor induced NF-κB activation. By effectively mimicking ligand-bound TNF receptor, API2-MALT1 promotes TRAF2-dependent ubiquitination of RIP1, which then acts as a scaffold for nucleating and activating the canonical NF-κB machinery. Activation occurs, in part, through MALT1 moiety-dependent recruitment of TRAF6, which can directly modify NF-κB essential modulator, the principal downstream regulator of NF-κB. While theintrinsic MALT1 protease catalytic activity is dispensable for this canonical NF-κB signaling, it is critical for noncanonical NF-κB activation. In this regard, API2-MALT1 recognizes NF-κB inducing kinase(NIK), the essential upstream regulator of non-canonical NF-κB, and cleaves it to generate

  16. MicroRNA-1 promotes apoptosis of hepatocarcinoma cells by targeting apoptosis inhibitor-5 (API-5).

    Science.gov (United States)

    Li, Dong; Liu, Yu; Li, Hua; Peng, Jing-Jing; Tan, Yan; Zou, Qiang; Song, Xiao-Feng; Du, Min; Yang, Zheng-Hui; Tan, Yong; Zhou, Jin-Jun; Xu, Tao; Fu, Zeng-Qiang; Feng, Jian-Qiong; Cheng, Peng; chen, Tao; Wei, Dong; Su, Xiao-Mei; Liu, Huan-Yi; Qi, Zhong-Chun; Tang, Li-Jun; Wang, Tao; Guo, Xin; Hu, Yong-He; Zhang, Tao

    2015-01-02

    Although microRNA-1 (miR-1) is a known liver cancer suppressor, the role of miR-1 in apoptosis of hepatoma cells has remained largely unknown. Our study shows that ectopic miR-1 overexpression induced apoptosis of liver hepatocellular carcinoma (HepG2) cells. Apoptosis inhibitor 5 (API-5) was found to be a potential regulator of miR-1 induced apoptosis, using a bioinformatics approach. Furthermore, an inverse relationship between miR-1 and API-5 expression was observed in human liver cancer tissues and adjacent normal liver tissues. Negative regulation of API-5 expression by miR-1 was demonstrated to promote apoptosis of HepG2 cells. Our study provides a novel regulatory mechanism of miR-1 in the apoptosis of hepatoma cells.

  17. The Design and Implementation of Cryptographic API%加密API的设计与实现

    Institute of Scientific and Technical Information of China (English)

    王军

    2004-01-01

    文章设计了一种简单易用、可扩展和可移植的加密API,简称SEPC-API.SEPC-API采用标准的C/C++来编写,并使用抽象密钥类分层结构来构造.文中详细描述了SEPC-API的设计和实现,并给出了应用示例.此外,SEPC-API中的多数函数设计遵循微软Crypto API的语法,这意味着微软Crypto API用户只需修改很少的代码就可以移植到SEPC-API.

  18. Espectro óculo-aurículo-vertebral em pacientes com defeitos cardíacos congênitos

    Directory of Open Access Journals (Sweden)

    Rafael Fabiano Machado Rosa

    2010-10-01

    Full Text Available FUNDAMENTO: Há poucos estudos avaliando a frequência do espectro óculo-aurículo-vertebral (EOAV nos pacientes com defeitos cardíacos congênitos (DCC. OBJETIVO: Verificar a frequência do EOAV em uma amostra de pacientes com malformações cardíacas graves. MÉTODOS: Avaliamos uma coorte prospectiva de pacientes com DCC, admitidos em uma unidade de terapia intensiva (UTI cardiopediátrica no Brasil. O diagnóstico de EOAV foi feito com base em dados clínicos, considerando critérios padrões. Os pacientes que preenchiam esses critérios foram submetidos ao cariótipo de alta resolução com bandamento GTG e hibridização fluorescente in situ para a microdeleção 22q11.2. O teste exato de Fisher (P < 0,05 foi usado para as análises estatísticas. RESULTADOS: Durante o período de avaliação, 330 pacientes foram hospitalizados pela primeira vez na UTI, mas trinta deles não participaram deste estudo. Dos 300 pacientes que constituíram a amostragem final, o EOAV foi verificado em três casos (1%. Todos apresentaram estudos citogenéticos normais. CONCLUSÃO: O EOAV parece ser uma condição frequente em pacientes com DCC. Contudo, não podemos excluir a possibilidade de que a frequência de EOAV encontrada em nosso estudo possa ter sido subestimada devido à baixa taxa de detecção pré-natal de DCC e ao acesso limitado dos pacientes ao tratamento médico apropriado em nosso meio. Estudos prospectivos futuros com critérios clínicos bem definidos e pacientes com defeitos leves e graves serão importantes para avaliar o papel do EOAV na população em geral de indivíduos com malformações cardíacas.

  19. AN OVERVIEW OF THE UNCERTAINTY ANALYSIS, SENSITIVITY ANALYSIS, AND PARAMETER ESTIMATION (UA/SA/PE) API AND HOW TO IMPLEMENT IT

    Science.gov (United States)

    The Application Programming Interface (API) for Uncertainty Analysis, Sensitivity Analysis, andParameter Estimation (UA/SA/PE API) (also known as Calibration, Optimization and Sensitivity and Uncertainty (CUSO)) was developed in a joint effort between several members of both ...

  20. An overview of the CellML API and its implementation

    Directory of Open Access Journals (Sweden)

    Halstead Matt

    2010-04-01

    Full Text Available Abstract Background CellML is an XML based language for representing mathematical models, in a machine-independent form which is suitable for their exchange between different authors, and for archival in a model repository. Allowing for the exchange and archival of models in a computer readable form is a key strategic goal in bioinformatics, because of the associated improvements in scientific record accuracy, the faster iterative process of scientific development, and the ability to combine models into large integrative models. However, for CellML models to be useful, tools which can process them correctly are needed. Due to some of the more complex features present in CellML models, such as imports, developing code ab initio to correctly process models can be an onerous task. For this reason, there is a clear and pressing need for an application programming interface (API, and a good implementation of that API, upon which tools can base their support for CellML. Results We developed an API which allows the information in CellML models to be retrieved and/or modified. We also developed a series of optional extension APIs, for tasks such as simplifying the handling of connections between variables, dealing with physical units, validating models, and translating models into different procedural languages. We have also provided a Free/Open Source implementation of this application programming interface, optimised to achieve good performance. Conclusions Tools have been developed using the API which are mature enough for widespread use. The API has the potential to accelerate the development of additional tools capable of processing CellML, and ultimately lead to an increased level of sharing of mathematical model descriptions.

  1. There-apy: The Use of Task, Imagery, and Symbolism To Connect the Inner and Outer Worlds.

    Science.gov (United States)

    Eisenstein-Naveh, A. Rosa

    2001-01-01

    Presents a model of therapy called there-apy, which weaves together the use of task, symbolism, and imagery into an ongoing process. Concrete tasks take on symbolic meaning, and symbolism gets actualized through achieving concrete tasks. There-apy connects the individual's outside and inside worlds and often involves the partner or family in the…

  2. Physicochemical characterisation and investigation of the bonding mechanisms of API-titanate nanotube composites as new drug carrier systems.

    Science.gov (United States)

    Sipos, Barbara; Pintye-Hódi, Klára; Kónya, Zoltán; Kelemen, András; Regdon, Géza; Sovány, Tamás

    2017-02-25

    Titanate nanotube (TNT) has recently been explored as a new carrier material for active pharmaceutical ingredients (API). The aim of the present work was to reveal the physicochemical properties of API-TNT composites, focusing on the interactions between the TNTs and the incorporated APIs. Drugs belonging to different Biopharmaceutical Classification System (BCS) classes were loaded into TNTs: diltiazem hydrochloride (BCS I.), diclofenac sodium (BCS II.), atenolol (BCS III.) and hydrochlorothiazide (BCS IV.). Experimental results demonstrated that it is feasible for spiral cross-sectioned titanate nanotubes to carry drugs and maintain their bioactivity. The structural properties of the composites were characterized by a range of analytical techniques, including FT-IR, DSC, TG-MS, etc. The interactions between APIs and TNTs were identified as electrostatic attractions, mainly dominated by hydrogen bonds. Based on the results, it can be stated that the strength of the association depends on the hydrogen donor strength of the API. The drug release of incorporated APIs was evaluated from compressed tablets and compared to that of pure APIs. Differences noticed in the dissolution profiles due to incorporation showed a correlation with the strength of interactions between the APIs and the TNTs observed in the above analytical studies.

  3. A pro-inflammatory role of C5L2 in C5a-primed neutrophils for ANCA-induced activation.

    Directory of Open Access Journals (Sweden)

    Jian Hao

    Full Text Available BACKGROUND: The complement system is crucial for the development of antineutrophil cytoplasmic antibody (ANCA-associated vasculitis (AAV. In particular, C5a and its receptor on neutrophils, CD88, play a central role. The functional role of the second receptor of C5a, C5L2, remains unclear. In the current study, we investigated the role of C5L2 in C5a-primed neutrophils for ANCA-induced activation. METHODS: The effect of blocking C5L2 by anti-human C5L2 blocking antibody were tested on respiratory burst and degranulation of C5a-primed neutrophils activated with ANCA, as well as on membrane-bound proteinase 3 (mPR3 and concentration of myeloperoxidase (MPO in supernatant of C5a-primed neutrophils. An antagonist for CD88 was also employed. RESULTS: Blocking C5L2 resulted in a significantly decreased MPO concentration in the supernatant of C5a-primed neutrophils. mPR3 expression increased from 209.0±43.0 in untreated cells to 444.3±60.8 after C5a treatment (P<0.001, and decreased to 375.8±65.44, 342.2±54.3 and 313.7±43.6 by pre-incubating blocking C5L2 antibody at 2.5 µg/ml, 5 µg/ml or 10 µg/ml (compared with C5a-priming group, P<0.001, P<0.001, and P<0.001, respectively. In C5a-primed neutrophils, subsequently activating with MPO-ANCA-positive IgG, the MFI value was 425.8±160.6, which decreased to 292.8±141.2, 289.7±130.0 and 280.3±136.4 upon pre-incubation with mouse anti-human C5L2 blocking antibody at 2.5 µg/ml, 5 µg/ml or 10 µg/ml (compared with C5a-primed neutrophils, for MPO-ANCA-positive IgG-induced activation, P<0.05, P<0.05, and P<0.05, respectively. Blocking C5L2 also resulted in significantly decreased C5a-primed neutrophils for PR3-ANCA-positive IgG-induced activation. Moreover, the lactoferrin concentration in the supernant significantly decreased in pre-incubation with anti-human C5L2 blocking antibody, compared with C5a-primed neutrophils induced by PR3- or MPO-ANCA-positive IgG. CONCLUSIONS: C5L2 may be implicated in

  4. Transcriptional regulation of aco gene cluster in Bacillus thuringiensis%苏云金芽胞杆菌3-羟基丁酮代谢基因簇的转录调控

    Institute of Scientific and Technical Information of China (English)

    黄闽忠; 彭琦; 张杰; 高继国; 宋福平

    2015-01-01

    [目的]通过分析苏云金芽胞杆菌(Bacillus thuringiensis)3-羟基丁酮代谢基因簇aco的转录调控和acoR突变体的表型特征,明确aco基因簇的转录调控机制和对芽胞产量及Cry蛋白产量的影响.[方法]通过生物信息学方法分析aco基因簇的结构,RT-PCR分析基因簇的转录单元,采用同源重组技术敲除苏云金芽胞杆菌HD73菌株的acoR基因,利用启动子融合lacZ的方法分析启动子的转录活性.利用总蛋白定量确定CrylAc蛋白产量.[结果]aco基因簇由acoABCL 4个基因组成,形成一个转录单元.aco基因簇的启动子PacoA转录活性在sigL(编码Sigma 54因子)和acoR突变体中均明显降低.acoR基因的缺失对菌体生长和CrylAc蛋白产量无显著影响,但使菌体运动能力减弱,使芽胞产量略有下降,并且不能利用3-羟基丁酮.[结论]aco操纵子受Sigma 54控制,并由AcoR激活,aocR基因的缺失影响菌体对3-羟基丁酮的利用,但对Cry蛋白产量无显著影响.

  5. Characterization of transcriptional profiles of MA-ACS1 and MA-ACO1 genes in response to ethylene, auxin, wounding, cold and different photoperiods during ripening in banana fruit.

    Science.gov (United States)

    Choudhury, Swarup Roy; Roy, Sujit; Sengupta, Dibyendu N

    2008-12-01

    The ripening-specific genes MA-ACS1 (Musa acuminata ACC synthase1) and MA-ACO1 (M. acuminata ACC oxidase 1) are regulated in response to a wide variety of factors. Here, we have studied the differential transcript accumulation pattern and protein levels of MA-ACS1 and MA-ACO1 genes in response to ethylene, auxin, wounding and low temperature in preclimacteric banana fruit. We have shown that exogenous application of ethylene and auxin induced the expression of MA-ACS1, while MA-ACO1 showed marginal expression following ethylene treatment in preclimacteric stage. Auxin did not induce MA-ACO1 expression. Thus, auxin-treated banana fruits showed lower ethylene production rate as compared to ethylene-treated fruits. Conversely, wounding and cold treatment down-regulated the expression of both the genes and thus inhibited ethylene production. Furthermore, we have detected a GCC-box putative ethylene-responsive element (ERE)- and an auxin-responsive element (ARE)-specific DNA-binding activity in the banana pulp and studied the ethylene and auxin responsive characteristics of the GCC-box and ARE (TGTCTC) containing synthetic promoter fragments. In addition, we have detected an enhanced ethylene production rate and expression level of MA-ACS1 and MA-ACO1 genes along with a strong GCC-box-specific DNA-binding activity following exposure to constant dark period for 8d at the preclimacteric stage. Together, our study provides interesting information about the regulation of expression of MA-ACS1 and MA-ACO1 genes in response to various factors during ripening in banana fruit, which may have physiological relevance concerning ethylene biosynthesis during post-harvest conditions.

  6. Is CK-MB isoenzyme useful for diagnosis of cardiac involvement in icteric leptospirosis? CK-MB: útil no diagnóstico de envolvimento cardíaco na leptospirose ictérica

    Directory of Open Access Journals (Sweden)

    Elizabeth Stankiewicz Machado

    1995-10-01

    Full Text Available In the absence of heart failure or cardiogenic shock, cardiac involvement diagnosis in icteric leptospirosis is possible on the basis of abnormal electrocardiograms. As metabolic and electrolytic disorders are frequently seen during acute leptospirosis infection, they may be responsible for some electrocardiograms changes. We conducted a study to assess if creatine phosphokinase isoenzyme determinations are useful in selecting patients with a high cardiac involvement suspicion. Sixty-nine patients were studied prospectively. Ten patients out of 16 with cardiac involvement and 25 without had high CK-MB levels (p>0.05, although mean values of abnormal CK-MB levels were higher in the group with cardiac involvement (pNa ausência de insuficiência cardíaca ou choque cardiogênico, o diagnóstico de envolvimento cardíaco na leptospirose ictérica pode ser baseado em alterações eletrocardiográficas. Devido ao frequente comprometimento multisistêmico da doença, algumas dessas alterações podem ser secundárias a distúrbios metabólicos ou eletrolíticos. Realizou-se um estudo para avaliação do significado da enzima CK-MB elevada em casos suspeitos de envolvimento cardíaco. Sessenta e nove pacientes com leptospirose ictérica foram estudados prospectivamente. Dez dos 16 casos com envolvimento cardíaco e 25 dos 53 pacientes sem envolvimento cardíaco tiveram CK-MB elevada (p>0,05. O valor médio de CK-MB foi maior no grupo com envolvimento cardíaco (p<0,05. Nossa análise indica que a presença de CK-MB elevada no curso de leptospirose ictérica não é indicadora de envolvimento cardíaco.

  7. A geographical information system using the Google Map API for guidance to referral hospitals.

    Science.gov (United States)

    Kobayashi, Shinji; Fujioka, Tetsushi; Tanaka, Yuji; Inoue, Michiyoshi; Niho, Yoshiyuki; Miyoshi, Akira

    2010-12-01

    Our hospital acts as a regional core hospital through inter-hospital collaboration. Geographical information is necessary to guide patients to the other hospitals. Although paper maps, which contain directions, nearest public transportation, etc., are usually provided to guide patients to the hospitals, the geographical information tends to change daily. However, updating the geographical information on the maps is costly. We constructed an electronic geographical information system using the Google Map API ( http://code.google.com/apis/maps/ ) with open source software to improve our ability to collaborate with other clinics.

  8. Oriental orchid (Cymbidium pumilum) attracts drones of the Japanese honeybee (Apis cerana japonica) as pollinators.

    Science.gov (United States)

    Sasaki, M; Ono, M; Asada, S; Yoshida, T

    1991-12-01

    The discovery that drones of the Japanese honeybee (Apis cerana japonica) pollinate the oriental orchid (Cymbidium pumilum) is reported. Drones are attracted to the orchid flower aroma mainly during their mating flights in April through May. Some drones cluster on the flower racemes and others insert their heads deep into the flowers. Drones with pollinia on their scutellum visit other orchids, which facilitates pollination. Individual workers and swarming colonies are also strongly attracted by the flower aroma, but the allopatric western honeybee (Apis mellifera) is not attracted.

  9. When is it Biased? Assessing the Representativeness of Twitter's Streaming API

    OpenAIRE

    Morstatter, Fred; Pfeffer, Jürgen; Liu, Huan

    2014-01-01

    Twitter has captured the interest of the scientific community not only for its massive user base and content, but also for its openness in sharing its data. Twitter shares a free 1% sample of its tweets through the "Streaming API", a service that returns a sample of tweets according to a set of parameters set by the researcher. Recently, research has pointed to evidence of bias in the data returned through the Streaming API, raising concern in the integrity of this data service for use in res...

  10. Develop 3G Application with The J2ME SATSA API

    Science.gov (United States)

    JunWu, Xu; JunLing, Liang

    This paper describes research in the use of the Security and Trust Services API for J2ME (SATSA) to develop mobile applications. for 3G networks. SATSA defines a set of APIs that allows J2ME applications to communicate with and access functionality, secure storage and cryptographic operations provided by security elements such as smart cards and Wireless Identification Modules (WIM). A Java Card application could also work as an authentication module in a J2ME-based e-bank application. The e-bank application would allow its users to access their bank accounts using their cell phones.

  11. Heterosis en la longevidad de obreras Apis mielífera

    Directory of Open Access Journals (Sweden)

    Soares AE.

    2001-06-01

    Full Text Available Se estimó la media y la heterosis de la longevidad en obreras de abejas Apis mellifera. El expe-rimento fue desarrollado en el Apiario del Departamento de Genética de la Facultad de Medi-cina de la USP-Ribeirão Preto-Brasil y en Jaboticabal-SP, de 03/1997 a 05/1999. Seleccionamos 8 matrices inseminadas del apiário-USP (2africanizadas, 2italianas, 2cárnicasy 2italianas*cárnicos y 2 matrices (grupo-testigo de abejas Africanizadas-Silvestres.

  12. Predominant Api m 10 sensitization as risk factor for treatment failure in honey bee venom immunotherapy

    DEFF Research Database (Denmark)

    Frick, Marcel; Fischer, Jörg; Helbling, Arthur;

    2016-01-01

    BACKGROUND: Component resolution recently identified distinct sensitization profiles in honey bee venom (HBV) allergy, some of which were dominated by specific IgE to Api m 3 and/or Api m 10, which have been reported to be underrepresented in therapeutic HBV preparations. OBJECTIVE: We performed...... a retrospective analysis of component-resolved sensitization profiles in HBV-allergic patients and association with treatment outcome. METHODS: HBV-allergic patients who had undergone controlled honey bee sting challenge after at least 6 months of HBV immunotherapy (n = 115) were included and classified...

  13. The Assessment of DNA in Royal Jelly from Apis mellifera and Apis cerana by RAPD markers%中蜂与意蜂王浆中DNA的RAPD分析

    Institute of Scientific and Technical Information of China (English)

    邹阳; 黄康; 颜伟玉; 曾志将

    2007-01-01

    以中华蜜蜂(Apis cerana cerana)和意大利蜜蜂( Apis mellifera ligustica)王浆为材料,用改进的苯酚-氯仿法提取蜂王浆中的DNA,利用35个随机引物对提取出来的DNA进行RAPD扩增,结果表明:有6个随机引物能扩增出谱带,其中1个引物对所有王浆DNA样品的扩增结果完全相同,5个引物出现多态性.共检测到32条扩增谱带,其中24条为多态带.

  14. Common celiacomesenteric trunk: a rare anatomic variation Tronco único celíaco-mesentérico: uma variante anatômica rara

    Directory of Open Access Journals (Sweden)

    K. Sridhar Varma

    2009-09-01

    Full Text Available Common celiacomesenteric trunk, with the celiac and superior mesenteric arteries having a common origin from the aorta, is the least frequently reported anatomic variation of all abdominal vascular anomalies. Knowledge of variations concerning the celiac trunk and superior mesenteric artery are of great importance for both surgical approaches and angiographic examinations. Clinicians should keep in mind these variations to avoid complications.O tronco único celíaco-mesentérico, com as artérias celíaca e mesentérica superior tendo uma origem comum a partir da aorta, é a variante anatômica menos reportada dentre todas as anomalias vasculares abdominais. Conhecer as variantes do tronco celíaco e da artéria mesentérica superior é de grande importância tanto para abordagens cirúrgicas quanto para exames angiográficos. É importante que os médicos tenham em mente essas variantes a fim de evitar complicações.

  15. Tamponamento cardíaco em dois recém-nascidos causado por cateter umbilical Cardiac tamponade caused by central venous catheter in two newborns

    Directory of Open Access Journals (Sweden)

    Andrey José Monteiro

    2008-09-01

    Full Text Available Tamponamento cardíaco secundário ao uso de cateter venoso central é uma complicação rara, porém potencialmente tratável, quando identificada a tempo. Nós relatamos dois casos de tamponamento cardíaco, diagnosticados por ecocardiograma transtorácico, seguido de pericardiocentese de urgência e drenagem pericárdica cirúrgica como complicação de cateterização venosa umbilical. Em um caso, a ponta do cateter estava adequadamente localizada e, no outro caso, não. Em ambos os casos, solução hiperosmolar estava sendo infundida. Apesar de situação incomum, esta deve ser sempre considerada em neonato, evoluindo com choque cardiogênico sem causa aparente.Cardiac tamponade secondary to the use of central venous catheter is a rare complication; however, it is potentially reversible when it is caught in time. We report two cases of cardiac tamponade that was diagnosed using a transthoracic echocardiography, followed by urgent pericardiocentesis and surgical pericardial drainage as a complication from umbilical venous catheterization. In one case, the tip of the catheter was properly placed, and in the other case, it was not. In both cases, a hyperosmolar solution was being injected. Although it may be an uncommon situation, it should be always considered as a possibility in a newborn who develops cardiogenic shock without an apparent cause.

  16. Tumores intracardíacos: aspectos generales y experiencia quirúrgica en el Hospital México

    Directory of Open Access Journals (Sweden)

    Eduardo Induni

    2002-04-01

    Full Text Available Objetivos Determinar la frecuencia de cirugías por tumores intracardíacos, asi como caracterizar los tipos histológicos, síntomas y evolución post cirugía. Material y Métodos De un total de 5.000 cirugías con circulación extracorpórea en el Hospital México, en el período de tiempo comprendido entre el 1 de enero de 1979 y el 1 de enero del 2001, se estudiaron retrospectivamente los datos relacionados con pacientes operados por tumores intracardíacos. En todos los casos se utilizó circulación extracorpórea con hipotermia moderada, cardioplejia cristaloide, realizándose cardiotomía y excisión amplia del tumor y de su base con reparación del defecto con PTFE ( Gore tex ® , cuando fue necesario. Resultados Un total de 20 resecciones tumorales fueron relizadas en 19 pacientes. Doce pacientes fueron hombres (63% y 7 mujeres (37%. El promedio de edad fue de 42.1 años siendo el menor de 16 años y el mayor de 85 años. Histológicamente 17 de ellos correspondieron a mixomas del atrio izquierdo (89%, uno presentó un quiste broncogénico del ventrículo derecho (5.5% y uno presentó un rabdomiosarcoma del septum interatrial que requirió excisión amplia del septum y reparación con parche (5.5%. Como procedimiento asociado además, se realizó una plastía de la válvula tricúspide y un by pass venoso a la arteria descendente anterior. La duración promedio de los síntomas fue de 2.8 meses, presentando datos de ICC en el 100% de los casos, dolor torácico en 16.5%, AVC embólico en el 11% y hemoptisis en el 5.5%. Todos los pacientes tuvieron diagnóstico ecocardiográfico preoperatorio. En la evolución post-operatoria se presentaron un AVC embólico (5,5% y un fallo multisistémico por sepsis en diabético que representa la mortalidad de los casos estudiados (5.5% y una estancia promedio post-quirúrgica de 6.2 días. Un paciente presentó una recidiva tumoral de mixoma a los 6 años post-cirugía la cual fue reoperada. Se

  17. Advance Payment ACO Model

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Advance Payment Model is designed for physician-based and rural providers who have come together voluntarily to give coordinated high quality care to the...

  18. GASTO CARDÍACO MATERNO COMO PREDICTOR DEL SÍNDROME DE PREECLAMPSIA-ECLAMPSIA / Maternal cardiac output as a predictor of preeclampsia-eclampsia syndrome

    Directory of Open Access Journals (Sweden)

    Luis A. Díaz Moreno

    2012-03-01

    Full Text Available Resumen Introducción y objetivos: La hipertensión es la complicación médica más común del embarazo, y puede dejar secuelas permanentes como: alteraciones neurológicas, hepáticas, hematológicas o renales. Cada tres minutos muere una mujer debido a la preeclampsia. Durante el embarazo el gasto cardíaco sufre un incremento de hasta 40 %. Este incremento es secundario al aumento de aproximadamente 30 % del volumen sistólico. El objetivo de la investigación fue predecir la aparición del síndrome de preeclampsia-eclampsia mediante la identificación de alteraciones del gasto cardíaco. Método: Se realizó un estudio observacional, descriptivo, longitudinal y analítico, de cohorte prospectiva, donde se determinó, por ecocardiografía, el gasto cardíaco en 31 embarazadas, entre las semanas 11 y 13,6 de gestación como único valor; y mediante seguimiento prenatal, se observó la aparición o no del síndrome de Preeclampsia-Eclampsia. Resultados: Se logró el seguimiento de 31 embarazadas a las que se le midió el gasto cardíaco, y se encontró una prevalecía de la enfermedad de 12,9 % con un 64,5 % de pacientes correctamente diagnosticados, con una sensibilidad de 75 %, con valor predictivo positivo de 23 %, especificidad de 62 % y valor predictivo negativo de 94%, con un riesgo relativo de 4,1 (intervalo de confianza 95 %, 0,48-35,6. Conclusiones: Es posible establecer la medición de gasto cardíaco como estudio de escrutinio para predecir la aparición del síndrome de Preeclampsia desde el control prenatal, y contribuir así a la disminución de la muerte materna. / Abstract Introduction and Objectives: Hypertension is the most common medical complication of pregnancy, and it can leave permanent sequelae such as neurological, hepatic, hematological or renal disorders. Every three minutes a woman dies due to preeclampsia. During pregnancy, cardiac output suffers an increase of 40%. This increase is secondary to an increase of

  19. Mixomas cardíacos: presentación clínica, resultados quirúrgicos y pronóstico a largo plazo

    Directory of Open Access Journals (Sweden)

    María N. Pizzi

    2010-01-01

    Full Text Available RESUMENIntroducciónLos tumores cardíacos primarios constituyen una patología poco frecuente y de ellos elmixoma es el más común. Con el progreso acelerado de los métodos por imágenes se haincrementado la frecuencia de su identificación in vivo. El cuadro clínico de presentaciónvaría ampliamente de acuerdo con el tamaño y la localización tumoral. En Latinoaméricason escasas las comunicaciones acerca de los resultados quirúrgicos de la resección demixomas cardíacos y del pronóstico a largo plazo.ObjetivosAnalizar la forma de presentación anatomoclínica, los resultados perioperatorios y la evoluciónde pacientes con mixoma cardíaco sometidos a resección quirúrgica.Material y métodosRevisión de 59 casos de mixomas cardíacos operados en nuestro centro entre 1992 y 2006.El seguimiento se realizó mediante consulta clínica, encuesta telefónica y ecocardiografía.ResultadosLa edad media fue de 53 ± 16,8 años. El 54,2% eran mujeres. La localización tumoral másfrecuente fue en la aurícula izquierda en el 81% de los casos. La forma clínica de presentaciónfue obstructiva en el 52,5%, embólica en el 37,2%, constitucional en el 27,1%, arritmiasupraventricular en el 22% y asintomática en el 10,1%. En dos casos (3,4% se trató derecurrencias tumorales. El diámetro tumoral mayor se relacionó con la presentaciónobstructiva y la arritmia supraventricular. El diámetro tumoral menor se asoció con la presentaciónembólica. La localización ventricular se observó en pacientes más jóvenes. Serealizó resección tumoral asociada con revascularización coronaria en el 8,4% y cirugíavalvular y/o de grandes vasos en el 13,5%. La mortalidad posoperatoria fue del 1,7% y lascomplicaciones más frecuentes fueron bloqueo auriculoventricular completo en el 23,7%,arritmia supraventricular en el 23,7% y bajo volumen minuto en el 18,6%. El seguimientoalejado se realizó en el 94,8% de los casos, con un promedio de 78,3 meses. El 65

  20. An example of how to handle amorphous fractions in API during early pharmaceutical development: SAR114137--a successful approach.

    Science.gov (United States)

    Petzoldt, Christine; Bley, Oliver; Byard, Stephen J; Andert, Doris; Baumgartner, Bruno; Nagel, Norbert; Tappertzhofen, Christoph; Feth, Martin Philipp

    2014-04-01

    The so-called pharmaceutical solid chain, which encompasses drug substance micronisation to the final tablet production, at pilot plant scale is presented as a case study for a novel, highly potent, pharmaceutical compound: SAR114137. Various solid-state analytical methods, such as solid-state Nuclear Magnetic Resonance (ssNMR), Differential Scanning Calorimetry (DSC), Dynamic Water Vapour Sorption Gravimetry (DWVSG), hot-stage Raman spectroscopy and X-ray Powder Diffraction (XRPD) were applied and evaluated to characterise and quantify amorphous content during the course of the physical treatment of crystalline active pharmaceutical ingredient (API). DSC was successfully used to monitor the changes in amorphous content during micronisation of the API, as well as during stability studies. (19)F solid-state NMR was found to be the method of choice for the detection and quantification of low levels of amorphous API, even in the final drug product (DP), since compaction during tablet manufacture was identified as a further source for the formation of amorphous API. The application of different jet milling techniques was a critical factor with respect to amorphous content formation. In the present case, the change from spiral jet milling to loop jet milling led to a decrease in amorphous API content from 20-30 w/w% to nearly 0 w/w% respectively. The use of loop jet milling also improved the processability of the API. Stability investigations on both the milled API and the DP showed a marked tendency for recrystallisation of the amorphous API content on exposure to elevated levels of relative humidity. No significant impact of amorphous API on either the chemical stability or the dissolution rate of the API in drug formulation was observed. Therefore, the presence of amorphous content in the oral formulation was of no consequence for the clinical trial phases I and II.

  1. Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)%桑树MaACS和MaACO基因的鉴定和表达模式研究

    Institute of Scientific and Technical Information of China (English)

    Chang-ying LIU; Shu-mei HAN; Cheng LU; Mao-de YU; Rui-hua LÜ; Jun LI; Ai-chun ZHAO; Xi-ling WANG; Umuhoza DIANE; Xiao-hong WANG; Chuan-hong WANG; Ya-sheng YU

    2014-01-01

    研究目的:分离和鉴定桑树中参与乙烯生物合成的酶的编码基因MaACS 和MaACO,研究其表达模式。创新要点:基于最新公布的桑树基因组数据库数据,获得5个MaACS 基因和2个MaACO 基因,对其进行了生物信息分析,同时鉴定了其在不同桑树组织中、不同发育时期桑椹中和不同激素作用下的表达模式。研究方法:通过生物信息学方法筛选和鉴定基因,利用荧光定量逆转录聚合酶链式反应(qRT-PCR)分析基因的表达量。重要结论:MaACS 和MaACO 基因在根、茎、叶等不同组织中呈现出不同的表达模式,在桑椹发育过程中呈现出两种表达模式,其表达量被脱落酸和乙烯利上调。%1-Aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal se-quences. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demon-strated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid (ABA) and ethephon.

  2. Insights into social insects from the genome of the honeybee Apis mellifera

    DEFF Research Database (Denmark)

    Hauser, Frank; Cazzamali, Giuseppe; Williamson, Michael

    2006-01-01

    Here we report the genome sequence of the honeybee Apis mellifera, a key model for social behaviour and essential to global ecology through pollination. Compared with other sequenced insect genomes, the A. mellifera genome has high A+T and CpG contents, lacks major transposon families, evolves mo...

  3. Sperm use economy of honeybee (Apis mellifera) queens

    DEFF Research Database (Denmark)

    Baer, Boris; Collins, Jason; Maalaps, Kristiina;

    2016-01-01

    the fecundity and longevity of queens and therefore colony fitness. We quantified the number of sperm that honeybee (Apis mellifera) queens use to fertilize eggs. We examined sperm use in naturally mated queens of different ages and in queens artificially inseminated with different volumes of semen. We found...

  4. Synergistic effects of non-Apis bees and honey bees for pollination services.

    Science.gov (United States)

    Brittain, Claire; Williams, Neal; Kremen, Claire; Klein, Alexandra-Maria

    2013-03-07

    In diverse pollinator communities, interspecific interactions may modify the behaviour and increase the pollination effectiveness of individual species. Because agricultural production reliant on pollination is growing, improving pollination effectiveness could increase crop yield without any increase in agricultural intensity or area. In California almond, a crop highly dependent on honey bee pollination, we explored the foraging behaviour and pollination effectiveness of honey bees in orchards with simple (honey bee only) and diverse (non-Apis bees present) bee communities. In orchards with non-Apis bees, the foraging behaviour of honey bees changed and the pollination effectiveness of a single honey bee visit was greater than in orchards where non-Apis bees were absent. This change translated to a greater proportion of fruit set in these orchards. Our field experiments show that increased pollinator diversity can synergistically increase pollination service, through species interactions that alter the behaviour and resulting functional quality of a dominant pollinator species. These results of functional synergy between species were supported by an additional controlled cage experiment with Osmia lignaria and Apis mellifera. Our findings highlight a largely unexplored facilitative component of the benefit of biodiversity to ecosystem services, and represent a way to improve pollinator-dependent crop yields in a sustainable manner.

  5. Gas chromatography coupled to atmospheric pressure ionization mass spectrometry (GC-API-MS): review.

    Science.gov (United States)

    Li, Du-Xin; Gan, Lin; Bronja, Amela; Schmitz, Oliver J

    2015-09-03

    Although the coupling of GC/MS with atmospheric pressure ionization (API) has been reported in 1970s, the interest in coupling GC with atmospheric pressure ion source was expanded in the last decade. The demand of a "soft" ion source for preserving highly diagnostic molecular ion is desirable, as compared to the "hard" ionization technique such as electron ionization (EI) in traditional GC/MS, which fragments the molecule in an extensive way. These API sources include atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), atmospheric pressure laser ionization (APLI), electrospray ionization (ESI) and low temperature plasma (LTP). This review discusses the advantages and drawbacks of this analytical platform. After an introduction in atmospheric pressure ionization the review gives an overview about the history and explains the mechanisms of various atmospheric pressure ionization techniques used in combination with GC such as APCI, APPI, APLI, ESI and LTP. Also new developments made in ion source geometry, ion source miniaturization and multipurpose ion source constructions are discussed and a comparison between GC-FID, GC-EI-MS and GC-API-MS shows the advantages and drawbacks of these techniques. The review ends with an overview of applications realized with GC-API-MS.

  6. Dispersibility of lactose fines as compared to API in dry powders for inhalation.

    Science.gov (United States)

    Thalberg, Kyrre; Åslund, Simon; Skogevall, Marcus; Andersson, Patrik

    2016-05-17

    This work investigates the dispersion performance of fine lactose particles as function of processing time, and compares it to the API, using Beclomethasone Dipropionate (BDP) as model API. The total load of fine particles is kept constant in the formulations while the proportions of API and lactose fines are varied. Fine particle assessment demonstrates that the lactose fines have higher dispersibility than the API. For standard formulations, processing time has a limited effect on the Fine Particle Fraction (FPF). For formulations containing magnesium stearate (MgSt), FPF of BDP is heavily influenced by processing time, with an initial increase, followed by a decrease at longer mixing times. An equation modeling the observed behavior is presented. Surprisingly, the dispersibility of the lactose fines present in the same formulation remains unaffected by mixing time. Magnesium analysis demonstrates that MgSt is transferred to the fine particles during the mixing process, thus lubrication both BDP and lactose fines, which leads to an increased FPF. Dry particle sizing of the formulations reveals a loss of fine particles at longer mixing times. Incorporation of fine particles into the carrier surfaces is believed to be behind this, and is hence a mechanism of importance as regards the dispersion performance of dry powders for inhalation.

  7. ApiNATOMY: a novel toolkit for visualizing multiscale anatomy schematics with phenotype-related information.

    Science.gov (United States)

    de Bono, Bernard; Grenon, Pierre; Sammut, Stephen John

    2012-05-01

    A significant proportion of biomedical resources carries information that cross references to anatomical structures across multiple scales. To improve the visualization of such resources in their anatomical context, we developed an automated methodology that produces anatomy schematics in a consistent manner,and provides for the overlay of anatomy-related resource information onto the same diagram. This methodology, called ApiNATOMY, draws upon the topology of ontology graphs to automatically lay out treemaps representing body parts as well as semantic metadata linking to such ontologies. More generally, ApiNATOMY treemaps provide an efficient and manageable way to visualize large biomedical ontologies in a meaningful and consistent manner. In the anatomy domain, such treemaps will allow epidemiologists, clinicians, and biomedical scientists to review, and interact with, anatomically aggregated heterogeneous data and model resources. Such an approach supports the visual identification of functional relations between anatomically colocalized resources that may not be immediately amenable to automation by ontology-based inferencing. We also describe the application of ApiNATOMY schematics to integrate, and add value to, human phenotype-related information—results are found at http://apinatomy.org. The long-term goal for the ApiNATOMY toolkit is to support clinical and scientific graphical user interfaces and dashboards for biomedical resource management and data analytics.

  8. Spatial and temporal variation of metal concentrations in adult honeybees (Apis mellifera L.)

    NARCIS (Netherlands)

    Steen, van der J.J.M.; Kraker, J.; Grotenhuis, J.T.C.

    2012-01-01

    Honeybees (Apis mellifera L.) have great potential for detecting and monitoring environmental pollution, given their wide-ranging foraging behaviour. Previous studies have demonstrated that concentrations of metals in adult honeybees were significantly higher at polluted than at control locations. T

  9. Testing of the structure of macromolecular polymer films containing solid active pharmaceutical ingredient (API) particles

    Energy Technology Data Exchange (ETDEWEB)

    Boelcskei, E. [Department of Pharmaceutical Technology, University of Szeged, H-6720 Szeged, Eoetvoes u. 6 (Hungary); Suevegh, K. [Laboratory of Nuclear Chemistry, Eoetvoes Lorand University, H-1518 Budapest 112, P.O. Box 32 (Hungary); Marek, T. [Hungarian Academy of Sciences, Research Group for Nuclear Techniques in Structural Chemistry, Eoetvoes Lorand University, H-1518 Budapest 112, P.O. Box 32 (Hungary); Regdon, G. [Department of Pharmaceutical Technology, University of Szeged, H-6720 Szeged, Eoetvoes u. 6 (Hungary); Pintye-Hodi, K., E-mail: klara.hodi@pharm.u-szeged.h [Department of Pharmaceutical Technology, University of Szeged, H-6720 Szeged, Eoetvoes u. 6 (Hungary)

    2011-07-15

    The aim of the present study was to investigate the structure of free films of Eudragit{sup L} 30D-55 containing different concentrations (0%, 1% or 5%) of diclofenac sodium by positron annihilation spectroscopy. The data revealed that the size of the free-volume holes and the lifetimes of ortho-positronium atoms decreased with increase of the API concentration. Films containing 5% of the API exhibited a different behavior during storage (17 {sup o}C, 65% relative humidity (RH)) in consequence of the uptake of water from the air. -- Highlights: {yields} The aim of the present study was to investigate the structure of free films of Eudragit{sup L} 30D-55 containing different concentrations (0%, 1% or 5%) of diclofenac sodium by positron annihilation spectroscopy. {yields} The data revealed that the size of the free-volume holes and the lifetimes of ortho-positronium atoms decreased with increase of the API concentration (). {yields} The API distorts the original polymer structure, but as time goes by, the metastable structure relaxes and it is almost totally restored after 3 weeks of storage (17 {sup o}C, 65% RH).

  10. ACME algorithms for contact in a multiphysics environment API version 2.2.

    Energy Technology Data Exchange (ETDEWEB)

    Heinstein, Martin Wilhelm; Glass, Micheal W.; Gullerud, Arne S.; Brown, Kevin H.; Voth, Thomas Eugene; Jones, Reese E.

    2004-07-01

    An effort is underway at Sandia National Laboratories to develop a library of algorithms to search for potential interactions between surfaces represented by analytic and discretized topological entities. This effort is also developing algorithms to determine forces due to these interactions for transient dynamics applications. This document describes the Application Programming Interface (API) for the ACME (Algorithms for Contact in a Multiphysics Environment) library.

  11. Reliability of the Advanced Psychodiagnostic Interpretation (API) Scoring System for the Bender Gestalt.

    Science.gov (United States)

    Aucone, Ernest J.; Raphael, Alan J.; Golden, Charles J.; Espe-Pfeifer, Patricia; Seldon, Jen; Pospisil, Tanya; Dornheim, Liane; Proctor-Weber, Zoe; Calabria, Michael

    1999-01-01

    Assessed the interrater reliability of the revised Advanced Psychodiagnostic Interpretation (API) (A. Raphael and C. Golden, 1998) scoring system for the Bender Gestalt Test (L. Bender, 1938). Agreement across nine raters exceeded 90% for each of three clinical protocols, and kappa statistics indicated good interrater reliability. (SLD)

  12. Identification of clinical yeasts by Vitek MS system compared with API ID 32 C.

    Science.gov (United States)

    Durán-Valle, M Teresa; Sanz-Rodríguez, Nuria; Muñoz-Paraíso, Carmen; Almagro-Moltó, María; Gómez-Garcés, José Luis

    2014-05-01

    We performed a clinical evaluation of the Vitek MS matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) system with the commercial database version 2.0 for rapid identification of medically important yeasts as compared with the conventional phenotypic method API ID 32 C. We tested 161 clinical isolates, nine isolates from culture collections and five reference strains. In case of discrepant results or no identification with one or both methods, molecular identification techniques were employed. Concordance between both methods was observed with 160/175 isolates (91.42%) and misidentifications by both systems occurred only when taxa were not included in the respective databases, i.e., one isolate of Candida etchellsii was identified as C. globosa by Vitek MS and two isolates of C. orthopsilosis were identified as C. parapsilosis by API ID 32 C. Vitek MS could not identify nine strains (5.14%) and API ID 32 C did not identify 13 (7.42%). Vitek MS was more reliable than API ID 32 C and reduced the time required for the identification of clinical isolates to only a few minutes.

  13. Continuous API-crystal coating via coacervation in a tubular reactor.

    Science.gov (United States)

    Besenhard, M O; Thurnberger, A; Hohl, R; Faulhammer, E; Rattenberger, J; Khinast, J G

    2014-11-20

    We present a proof-of-concept study of a continuous coating process of single API crystals in a tubular reactor using coacervation as a microencapsulation technique. Continuous API crystal coating can have several advantages, as in a single step (following crystallization) individual crystals can be prepared with a functional coating, either to change the release behavior, to protect the API from gastric juice or to modify the surface energetics of the API (i.e., to tailor the hydrophobic/hydrophilic characteristics, flowability or agglomeration tendency, etc.). The coating process was developed for the microencapsulation of a lipophilic core material (ibuprofen crystals of 20 μm- to 100 μm-size), with either hypromellose phthalate (HPMCP) or Eudragit L100-55. The core material was suspended in an aqueous solution containing one of these enteric polymers, fed into the tubing and mixed continuously with a sodium sulfate solution as an antisolvent to induce coacervation. A subsequent temperature treatment was applied to optimize the microencapsulation of crystals via the polymer-rich coacervate phase. Cross-linking of the coating shell was achieved by mixing the processed material with an acidic solution (pH<3). Flow rates, temperature profiles and polymer-to-antisolvent ratios had to be tightly controlled to avoid excessive aggregation, leading to pipe plugging. This work demonstrates the potential of a tubular reactor design for continuous coating applications and is the basis for future work, combining continuous crystallization and coating.

  14. Does Year Round Schooling Affect the Outcome and Growth of California's API Scores?

    Science.gov (United States)

    Wu, Amery D.; Stone, Jake E.

    2010-01-01

    This paper examined whether year round schooling (YRS) in California had an effect upon the outcome and growth of schools' Academic Performance Index (API) scores. While many previous studies had examined the connection between YRS and academic achievement, most had lacked the statistical rigour required to provide reliable interpretations. As a…

  15. An Auto-Configuration System for the GMSEC Architecture and API

    Science.gov (United States)

    Moholt, Joseph; Mayorga, Arturo

    2007-01-01

    A viewgraph presentation on an automated configuration concept for The Goddard Mission Services Evolution Center (GMSEC) architecture and Application Program Interface (API) is shown. The topics include: 1) The Goddard Mission Services Evolution Center (GMSEC); 2) Automated Configuration Concept; 3) Implementation Approach; and 4) Key Components and Benefits.

  16. Responses of Varroa-resistant honey bees (Apis mellifera L.) to Deformed wing virus

    Science.gov (United States)

    The negative impact of Deformedwing virus (DWV) on European honey bees Apis mellifera is magnified by Varroa destructor parasitism. This study compared the responses of two Varroa-resistant honey bee stocks, pure Russian honey bees (RHB) and out-crossed Varroa Sensitive Hygienic bees, Pol-line (POL)...

  17. Young and old honey bee (Apis mellifera) larvae differentially prime the developmental maturation of their caregivers

    Science.gov (United States)

    In eusocial insects daughters rear the offspring of the queen to adulthood. In the honey bee, Apis mellifera, nurses differentially regulate larval nutrition. Among worker-destined larvae, younger instars receive an unrestricted diet paralleling that of queen larvae in protein composition but with r...

  18. Involvement of Phosphorylated "Apis Mellifera" CREB in Gating a Honeybee's Behavioral Response to an External Stimulus

    Science.gov (United States)

    Gehring, Katrin B.; Heufelder, Karin; Feige, Janina; Bauer, Paul; Dyck, Yan; Ehrhardt, Lea; Kühnemund, Johannes; Bergmann, Anja; Göbel, Josefine; Isecke, Marlene; Eisenhardt, Dorothea

    2016-01-01

    The transcription factor cAMP-response element-binding protein (CREB) is involved in neuronal plasticity. Phosphorylation activates CREB and an increased level of phosphorylated CREB is regarded as an indicator of CREB-dependent transcriptional activation. In honeybees ("Apis mellifera") we recently demonstrated a particular high…

  19. Polymorphic DNA sequences of the fungal honey bee pathogen Ascosphaera apis

    DEFF Research Database (Denmark)

    Jensen, Annette B; Welker, Dennis L; Kryger, Per

    2012-01-01

    was then compared among the different loci, and three were found to have the greatest detection power for identifying A. apis haplotypes. The described loci can help to resolve strain differences and population genetic structures, to elucidate host–pathogen interaction and to test evolutionary hypotheses...

  20. Enabling Mobile Air Quality App Development with an AirNow API

    Science.gov (United States)

    Dye, T.; White, J. E.; Ludewig, S. A.; Dickerson, P.; Healy, A. N.; West, J. W.; Prince, L. A.

    2013-12-01

    The U.S. Environmental Protection Agency's (EPA) AirNow program works with over 130 participating state, local, and federal air quality agencies to obtain, quality control, and store real-time air quality observations and forecasts. From these data, the AirNow system generates thousands of maps and products each hour. Each day, information from AirNow is published online and in other media to assist the public in making health-based decisions related to air quality. However, an increasing number of people use mobile devices as their primary tool for obtaining information, and AirNow has responded to this trend by publishing an easy-to-use Web API that is useful for mobile app developers. This presentation will describe the various features of the AirNow application programming interface (API), including Representational State Transfer (REST)-type web services, file outputs, and RSS feeds. In addition, a web portal for the AirNow API will be shown, including documentation on use of the system, a query tool for configuring and running web services, and general information about the air quality data and forecasts available. Data published via the AirNow API includes corresponding Air Quality Index (AQI) levels for each pollutant. We will highlight examples of mobile apps that are using the AirNow API to provide location-based, real-time air quality information. Examples will include mobile apps developed for Minnesota ('Minnesota Air') and Washington, D.C. ('Clean Air Partners Air Quality'), and an app developed by EPA ('EPA AirNow').

  1. Alteration Summary of the 8th Edition API SPEC Q1 as Opposed to the 7th Edition%API SPEC Q1第8版相对第7版变更摘要

    Institute of Scientific and Technical Information of China (English)

    方晓东

    2009-01-01

    @@ API SPEC Q1是API标准中一项重要的技术政策性标准,通用于API的所有产品规范.API Q1第8版于2007年12月发布, 2008年6月15日生效.2008年6月15日以后对申请会标公司质量管理体系的审核,将按API Q1第8版的要求进行.

  2. Small Molecule APY606 Displays Extensive Antitumor Activity in Pancreatic Cancer via Impairing Ras-MAPK Signaling

    Science.gov (United States)

    Guo, Na; Liu, Zuojia; Zhao, Wenjing; Wang, Erkang; Wang, Jin

    2016-01-01

    Pancreatic cancer has been found with abnormal expression or mutation in Ras proteins. Oncogenic Ras activation exploits their extensive signaling reach to affect multiple cellular processes, in which the mitogen-activated protein kinase (MAPK) signaling exerts important roles in tumorigenesis. Therapies targeted Ras are thus of major benefit for pancreatic cancer. Although small molecule APY606 has been successfully picked out by virtual drug screening based on Ras target receptor, its in-depth mechanism remains to be elucidated. We herein assessed the antitumor activity of APY606 against human pancreatic cancer Capan-1 and SW1990 cell lines and explored the effect of Ras-MAPK and apoptosis-related signaling pathway on the activity of APY606. APY606 treatment resulted in a dose- and time-dependent inhibition of cancer cell viability. Additionally, APY606 exhibited strong antitumor activity, as evidenced not only by reduction in tumor cell invasion, migration and mitochondrial membrane potential but also by alteration in several apoptotic indexes. Furthermore, APY606 treatment directly inhibited Ras-GTP and the downstream activation of MAPK, which resulted in the down-regulation of anti-apoptotic protein Bcl-2, leading to the up-regulation of mitochondrial apoptosis pathway-related proteins (Bax, cytosolic Cytochrome c and Caspase 3) and of cyclin-dependent kinase 2 and Cyclin A, E. These data suggest that impairing Ras-MAPK signaling is a novel mechanism of action for APY606 during therapeutic intervention in pancreatic cancer. PMID:27223122

  3. Pim-2 activates API-5 to inhibit the apoptosis of hepatocellular carcinoma cells through NF-kappaB pathway.

    Science.gov (United States)

    Ren, Ke; Zhang, Wei; Shi, Yujun; Gong, Jianping

    2010-06-01

    Pim-2 is proved to be relevant to the tumorigenesis of hepatocellular carcinoma (HCC), but the mechanism is unclear. We studied the relationship among Pim-2, NF-kappaB and API-5. In our experiment, expression level of the three factors and phosphorylation level of API-5, as well as NF-kappaB activity, were detected in HCC tissues and the nontumorous controls. Then Pim-2 gene was transfected into nontumorous liver cells L02, and Pim-2 SiRNA was transfected into hepatoblastoma cell line HepG2. Parthenolide was added as NF-kappaB inhibitor. The same detections as above were repeated in the cells, along with the apoptosis analysis. We found the levels of Pim-2, NF-kappaB and API-5, as well as NF-kappaB activity, were significantly higher in HCC tissues. Pim-2 level was increased in L02 cells after the transfection of Pim-2 gene, but decreased in HepG2 cells after the transfection of Pim-2 SiRNA. The levels of NF-kappaB and API-5, as well as NF-kappaB activity and API-5 phosphorylation level, were in accordance with Pim-2 level, but could be reversed by Parthenolide. Cell apoptosis rates were negatively correlated with API-5 phosphorylation level. Therefore, we infer that Pim-2 could activate API-5 to inhibit the apoptosis of liver cells, and NF-kappaB is the key regulator.

  4. Transplante experimental cardíaco heterotópico e cutâneo em camundongos Experimental heterotopic cardiac and cutaneous transplantation in mice

    Directory of Open Access Journals (Sweden)

    Patrícia Sestrheim

    2005-06-01

    Full Text Available OBJETIVO: Estudo experimental com o objetivo de desenvolver e avaliar a viabilidade das técnicas de transplante experimental cardíaco heterotópico abdominal vascularizado e cutâneo em camundongos, criando um instrumento para investigação da eficácia de soluções de preservação, novas drogas imunossupressoras, agentes biológicos, terapia gênica e indução de tolerância imunológica. MÉTODO: Para este estudo, as técnicas utilizadas foram descritas previamente por Corry et al. e Billingham et al. RESULTADOS: O tempo cirúrgico total para a realização dos transplantes cardíacos (n=20 foi, em média, 60,3±6,3 minutos e para os transplantes cutâneos (n=20, 17,75±0,71 minutos. A média de sobrevida dos aloenxertos cutâneos (n=34 e cardíacos (n=24 foi, respectivamente, 7 e 11 dias, enquanto que os isoenxertos sobreviveram por mais de 100 dias. CONCLUSÕES: Ambas as técnicas se caracterizaram pela fácil reprodutibilidade dos modelos experimentais. As diferenças entre as técnicas não se limitaram às peculiaridades metodológicas ou ao tempo de sobrevida e vascularização, mas principalmente à sua imunogenicidade e suscetibilidade à rejeição.OBJECTIVE: This is an experimental study which aims at developing and evaluating the feasibility of experimental techniques of vascularized and cutaneous abdominal heterotopic heart transplant in mice, creating an instrument of investigation for the effectiveness of prservation solutions, new immunosuppressive drugs, biological agents, genetic therapy and induction of immunological tolerance. METHOD: The techniques used in this work were previously described by Corry et al. and Billingham et al. RESULTS: The total surgical time to perform the cardiac transplants (n=20 was on average 60.3+6.3 minutes and the time of cutaneous transplants (n= 20 17.75+0.71 minutes. The average survival of the cutaneous allografts (n=34 and cardiac (n=24 allografts was 7 and 11 days, respectively, while

  5. Avaliação do sopro cardíaco na infância Assessment of heart murmurs in childhood

    Directory of Open Access Journals (Sweden)

    Maria Elisabeth B.A. Kobinger

    2003-06-01

    Full Text Available OBJETIVO: discutir a avaliação clínica e laboratorial do sopro cardíaco, considerando sua alta freqüência no atendimento ambulatorial do pediatra geral. FONTE DE DADOS: revisão baseada na análise crítica da literatura atual e consulta a compêndios de cardiologia pediátrica e pediatria contendo informações básicas sobre o tema. SÍNTESE DOS DADOS: os principais destaques do artigo referem-se à importância da anamnese e exame físico do sistema cardiovascular, essenciais para o pediatra geral diagnosticar o sopro cardíaco inocente, e identificar situações que indiquem a ocorrência de cardiopatias congênitas, ou adquiridas, e a necessidade de encaminhamento ao especialista. CONCLUSÕES: o pediatra geral é geralmente o primeiro médico a detectar um sopro cardíaco e deve estar apto a reconhecer o sopro inocente, assim como a suspeitar precocemente de doenças cardiovasculares.OBJECTIVE: to discuss clinical and laboratorial evaluation of heart murmurs in children, an important problem faced by pediatricians in their practice. SOURCES OF DATA: this review was based on a critical analysis of the current literature, as well as pediatrics and pediatric cardiology textbooks, which were found to be an important source of information on the subject. SUMMARY OS THE FINDINGS: it is important for pediatricians to know how to obtain precise information regarding the patient's medical history and to perform extensive physical examination of a child with heart murmur. The diagnosis of innocent heart murmur is essentially clinical and it can help the pediatrician to identify situations which are associated with cardiovascular diseases. CONCLUSIONS: in our series, short-term video-EEG monitoring established a reliable diagnosis in most patients due to correlation between clinical and EEG data. This procedure was well tolerated by children, including infants and those with psychiatric disorders.

  6. Cardiac beta-receptors in experimental Chagas' disease Receptores beta cardíacos na doença de Chagas experimental

    Directory of Open Access Journals (Sweden)

    Julio E. Enders

    1995-02-01

    Full Text Available Experimental Chagas' disease (45 to 90 days post-infection showed serious cardiac alterations in the contractility and in the pharmacological response to beta adrenergic receptors in normal and T. cruzi infected mice (post-acute phase. Chagasic infection did not change the beta receptors density (78.591 ± 3.125 fmol/mg protein and 73.647 ± 2.194 fmol/mg protein for controls but their affinity was significantly diminished (Kd = 7.299 ± 0.426 nM and Kd = 3.759 ± 0.212 nM for the control p Estudaram-se os receptores beta cardíacos de camundongos infectados pelo Trypanosoma cruzi na fase pós-aguda da doença de Chagas para estabelecer em que medida os mesmos contribuem a gerar respostas anômalas às catecolaminas observadas nestes miocardios. Utilizara-se 3-H/DHA para a marcação dos receptores beta cardíacos dos camundongos normais e dos infectados na fase pós-aguda (45 a 90 dias pós-infecção. O número dos sítios de fixação foi similar nos dois grupos, 78.591 ± 3.125 fmol/mg. Proteína nos chagásicos e 73.647 ± 2.194 fmol/mg. Proteína no grupo controle. Em vez disso, a afinidade verificou-se significativamente diminuida no grupo chagásico (Kd = 7.299 ± 0.426 nM respeito do controle (Kd = 3.759 ± 0.212 nM p < 0.001. Os resultados obtidos demonstram que as modificações observadas na estimulação adrenérgica do miocárdio chagásico se correlacionam com a menor afinidade dos receptores beta cardíacos e que estas alterações exerceriam uma parte determinante para as consequências funcionais que são detectadas na fase crônica.

  7. O transplante cardíaco biatrial deve ainda ser realizado? Metanálise Should biatrial heart transplantation still be performed? A Meta-analysis

    Directory of Open Access Journals (Sweden)

    Rafael Fagionato Locali

    2010-06-01

    Full Text Available As técnicas de transplante cardíaco bicaval e total apresentam melhores resultados que a biatrial, porém esta ainda é considerada o padrão-ouro. O objetivo é determinar se as técnicas de transplante cardíaco bicaval e total são, de fato, melhores que a técnica biatrial. Realizou-se a revisão sistemática com metanálise. Os estudos foram provenientes das bases de dados da Pubmed®, Lilacs®, Web of Science®, Scirus®, Scopus®, Google Acadêmico® e Scielo®, identificados por estratégia sensível. Elegeram-se, para a inclusão, estudos aleatórios e estudos prospectivos e retrospectivos controlados. Parâmetros intra e pós-operatórios foram avaliados. Foram identificados 11.602 estudos, e 36 foram incluídos na revisão. O número de arritmias atriais, insuficiência valvar tricúspide, mortalidade, eventos embólicos, volume de sangramento, necessidade de marcapasso temporário e permanente e o tempo de estada em unidade de terapia intensiva são significativamente menores nas técnicas bicaval e total do que na biatrial. Além disso, variáveis hemodinâmicas como a pressão capilar pulmonar, pressão média de artéria pulmonar e pressão de átrio direito são menores nos transplantes bicaval e total. Os transplantes cardíacos ortotópicos bicaval e total são melhores, em termos de prognóstico, que o biatrial. Portanto, a indicação da técnica biatrial para transplante deve ser a exceção e não a regra.

  8. Endovascular repair of an aorto-iliac aneurysm succeeded by kidney transplantation Tratamento endovascular de aneurisma aorto-ilíaco sucedido por transplante renal

    Directory of Open Access Journals (Sweden)

    Marcelo Bellini Dalio

    2010-09-01

    Full Text Available We present the case of aorto-iliac aneurysm in a patient with chronic renal failure requiring dialysis who were treated with an endovascular stent graft and, later on, submitted to kidney transplantation. A 53-year-old male with renal failure requiring dialysis presented with an asymptomatic abdominal aorto-iliac aneurysm measuring 5.0cm of diameter. He was treated with endovascular repair technique, being used an endoprosthesis Excluder®. After four months, he was successfully submitted to kidney transplantation (dead donor, with anastomosis of the graft renal artery in the external iliac artery distal to the endoprosthesis. The magnetic resonance imaging, carried out 30 days after the procedure, showed a good positioning of the endoprosthesis and adequate perfusion of the renal graft. In the follow-up, the patient presented improvement of nitrogenous waste, good positioning of the endoprosthesis without migration or endoleak. The endovascular repair of aorto-iliac aneurysm in a patient with end-stage renal failure under hemodialysis treatment showed to be feasible, safe and efficient, as it did not prevent the success of the posterior kidney transplantation.Apresentamos o caso de aneurisma aortoilíaco em um paciente com insuficiência renal crônica dialítica tratado com uma endoprótese vascular, sendo, após, submetido a transplante renal. Um homem de 53 anos com insuficiência renal dialítica apresentava um aneurisma abdominal aortoilíaco assintomático com 5,0cm de diâmetro. Foi tratado com técnica endovascular com uma endoprótese Excluderâ. Após quatro meses, foi submetido a transplante renal (doador cadáver com sucesso, com anastomose da artéria renal do enxerto na artéria ilíaca externa distal à endoprótese. A ressonância magnética 30 dias após o procedimento mostrou a endoprótese bem posicionada e o enxerto renal bem perfundido. No seguimento, o paciente evoluiu com melhora das escórias nitrogenadas, bom

  9. Evolução a longo prazo e complicações da hipertensão arterial após transplante cardíaco

    Directory of Open Access Journals (Sweden)

    Bortolotto Luiz Aparecido

    1997-01-01

    Full Text Available OBJETIVO: Avaliar a evolução da hipertensão arterial (HA, e suas consequências, em pacientes submetidos a transplante cardíaco (TC em uso de ciclosporina (CL. MÉTODOS: Em 65 pacientes submetidos a TC ortotópico, avaliamos a pressão arterial, creatinina sérica e níveis sangüíneos de CL nos períodos pré-operatório (15 dias antes do TC, pós-operatório precoce (15 e 30 dias e tardio (6, 12, 24, 48 e 60 meses; em 20 pacientes analisamos índice cardíaco e resistência vascular no pré, 15 e 30 dias, 6 e 12 meses após TC; em 33 pacientes, estudamos estrutura e função ventricular ao ecocardiograma, 24±13 meses após TC. RESULTADOS: Após 30 dias, a HA estava presente em 58,5% dos pacientes (50% leve, enquanto na evolução tardia, a incidência da HA aumentou significativamente para 93% após um ano (85% moderada a grave. A creatinina sérica aumentou progressivamente do pré-TC (1,43±0,5mg/dl até após um ano (1,83± 0,9 mg/dl, mantendo leve incremento até os 60 meses (2,4± 0,8mg/dl. Não houve correlação entre a HA, creatinina sérica e níveis de CL. O índice cardíaco aumentou na fase precoce, enquanto a resistência periférica diminuiu no início e aumentou significativamente aos 12 meses. Ao ecocardiograma, 54% dos pacientes apresentavam hipertrofia de ventrículo esquerdo com função normal. Dos 31 pacientes que faleceram durante a evolução, dois tiveram a causa mortis diretamente relacionada a HA. CONCLUSÃO: A HA em pacientes submetidos a TC em uso de CL ocorre precocemente, aumenta em prevalência e gravidade com o tempo e é mediada por aumento da resistência periférica, não se correlacionando com a nefrotoxicidade e com os níveis sangüíneos da CL, podendo agravar a insuficiência renal ou comprometer a longevidade do transplante, induzindo hipertrofia ventricular.

  10. Tamponamento cardíaco tardio traumático: análise de cinco casos Traumatic late cardiac tamponade: analysis of five cases

    Directory of Open Access Journals (Sweden)

    FERNANDO LUIZ WESTPHAL

    2000-09-01

    Full Text Available São analisados cinco casos de tamponamento cardíaco tardio traumático. Os pacientes eram masculinos, idade média de 26,2 anos, vítimas de ferimento por arma branca em região precordial, área de Ziedler, sendo admitidos em centro de referência para trauma. Foram classificados pelo índice fisiológico de Ivatury para trauma cardíaco e inicialmente tratados por pleurotomia intercostal e reposição volêmica, com estabilização do quadro hemodinâmico e respiratório. Os pacientes foram readmitidos após intervalo de oito a 24 dias (mediana de 20 dias, agora no serviço de cirurgia torácica de um hospital de referência terciária, com sinais de tamponamento cardíaco. Os exames diagnósticos confirmaram derrame pericárdico com espessamento pericárdico associado a encarceramento de base pulmonar esquerdo em quatro casos, os quais foram abordados por toracotomia póstero-lateral, com realização de pericardiectomia parcial e descorticação pulmonar. Um paciente evoluiu com pericardite purulenta, comprovada por exames complementares, e foi submetido à drenagem pericárdica subxifóidea. Ocorreu arritmia pós-operatória em um paciente; os demais evoluíram sem complicações pós-operatórias ou recidiva do tamponamento.Five traumatic late cardiac tamponade cases were analyzed. All patients were male, mean age was 26.2, victims of thoracic penetrating stabbing wound in the precordial region, Ziedler area, admitted to a trauma reference center. They were classified by the Ivatury physiological index for cardiac trauma. The first treatment approach was intercostal pleurectomy and volemic resuscitation followed by hemodynamic and respiratory recovery. Patients with cardiac tamponade symptoms were re-admitted within an interval from eight to twenty four days (mean 20 days in a thoracic surgery service of a tertiary reference hospital. Diagnostic exams confirmed thickening and pericardial effusion associated with a left pulmonary base

  11. 改进的牵引移动的蚁群算法在2D HP 模型中的应用%APPLYING IMPROVED PULL MOVES ACO IN 2D HP MODEL

    Institute of Scientific and Technical Information of China (English)

    高翔; 杨惠; 张成文

    2015-01-01

    Protein folding problem is to predict a protein’s conformation from its amino acid sequence,it is one of the most prominent problems in bioinformatics.In this paper we mainly study two-dimensional (2D)hydrophobic-polar (HP)lattice model,which is a representative simplified model widely used for simulating the protein folding problem,and apply the ant colony optimisation (ACO)to solving the 2D HP protein folding problem.Besides,we introduce an improved pull moves method to local search mechanism,to improve protein’s conformation it is an effective method.Experimental results show that the improved ACO with pull moves (ACO +)can obtain lower-energy conformations than ACO for several longer amino acid sequences,this demonstrates that our ACO + algorithm is a effective method to predict the structure of protein.%蛋白质折叠问题就是从氨基酸序列中预测蛋白质的构象,该问题是生物信息学的一个突出问题。主要研究二维 HP 格点模型,它是用于模拟蛋白质折叠问题的一个具有代表性的简化模型,并且将蚁群算法用于求解该二维 HP 蛋白质的折叠问题。此外,在局部搜索机制中引入一种改进的牵引移动方法,这是一个提高蛋白质构象的有效方法。实验结果表明,针对较长的氨基酸序列,改进的带牵引移动的蚁群算法(ACO +)比 ACO 能够获得更低能量的构象,证明了所提出的改进蚁群算法是预测蛋白质结构的有效方法。

  12. Bioimpedância transtorácica comparada à ressonância magnética na avaliação do débito cardíaco

    Directory of Open Access Journals (Sweden)

    Humberto Villacorta Junior

    Full Text Available FUNDAMENTO: A ressonância magnética cardíaca é considerada o método padrão-ouro para o cálculo de volumes cardíacos. A bioimpedância transtorácica cardíaca avalia o débito cardíaco. Não há trabalhos que validem essa medida comparada à ressonância. OBJETIVO: Avaliar o desempenho da bioimpedância transtorácica cardíaca no cálculo do débito cardíaco, índice cardíaco e volume sistólico, utilizando a ressonância como padrão-ouro. MÉTODOS: Avaliados 31 pacientes, com média de idade de 56,7 ± 18 anos, sendo 18 (58% do sexo masculino. Foram excluídos os pacientes cuja indicação para a ressonância magnética cardíaca incluía avaliação sob estresse farmacológico. A correlação entre os métodos foi avaliada pelo coeficiente de Pearson, e a dispersão das diferenças absolutas em relação à média foi demonstrada pelo método de Bland-Altman. A concordância entre os métodos foi realizada pelo coeficiente de correlação intraclasses. RESULTADOS: A média do débito cardíaco pela bioimpedância transtorácica cardíaca e pela ressonância foi, respectivamente, 5,16 ± 0,9 e 5,13 ± 0,9 L/min. Observou-se boa correlação entre os métodos para o débito cardíaco (r = 0,79; p = 0,0001, índice cardíaco (r = 0,74; p = 0,0001 e volume sistólico (r = 0,88; p = 0,0001. A avaliação pelo gráfico de Bland-Altman mostrou pequena dispersão das diferenças em relação à média, com baixa amplitude dos intervalos de concordância. Houve boa concordância entre os dois métodos quando avaliados pelo coeficiente de correlação intraclasses, com coeficientes para débito cardíaco, índice cardíaco e volume sistólico de 0,78, 0,73 e 0,88, respectivamente (p < 0,0001 para todas as comparações. CONCLUSÃO: A bioimpedância transtorácica cardíaca mostrou-se acurada no cálculo do débito cardíaco quando comparada à ressonância magnética cardíaca.

  13. Un sistema presión-volumen para la medición de propiedades mecánicas de vasos cardíacos menores

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    Javier Navarro Rueda

    2013-04-01

    Full Text Available Las propiedades mecánicas de vasos cardíacos menores (VM son poco estudiadas. El sistema Presión-Volumen (PV propuesto, desarrollado para evaluar comportamientos esfuerzo-deformación (σ-ε de VM, permite variar controladamente la presión interna y simular alteraciones geométricas al tubo. Se obtuvieron imágenes de las geometrías para cada valor de presión interna. Asumiendo el tubo en equilibrio, con pared elástica y presiones conocidas, se determinaron esfuerzo y deformación circunferenciales. Se obtuvieron curvas σ-ε, validadas con ensayos de tensión uniaxial (TU. Aunque se esperaban comportamientos diferentes, los datos experimentales son consistentes frente a resultados TU, con diferencias entre 5.68 y 12.60 %.

  14. Investigação de lipofuscina em miócitos cardíacos de camundongos após tratamento com oxamniquine

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    Maria Luiza Silveira Mello

    1984-10-01

    Full Text Available A fluorescencia natural devida à presença de corpos de lipofuscina foi pesquisada em miócitos cardíacos de camundongos adultos jovens tratados com oxamniquine. O objetivo foi buscar uma evidência de indução de envelhecimento celular precoce, com base em dados prévios de acentuação poliploidia, promovida pela droga. Comparando o miocárdio dos camundongos tratados com animais contrôle jovens e velhos, não foi observada a presença de lipofuscina nos seus miócitos. Isto possivelmente se deva a um não comprometimento da eficiência das lipases lisossomiais apesar da acentuação do fenômeno de poliploidização induzida pela droga.

  15. Efeitos da mutaçao da tropomiosina Glu180Gly nos parâmetros de contratilidade do músculo estriado cardíaco

    OpenAIRE

    Szkudlarek, Ariani Cavazzani

    2012-01-01

    A Cardiomiopatia Hipertrófica Familiar (FHC) é causada por uma mutação em uma das proteínas do aparato contrátil cardíaco, incluindo a a - tropomiosina (alfa - TM). Este estudo usou músculo papilar esquerdo de camundongos transgênicos (TG) com uma mutação na a - TM, sendo esta uma substituição de aminoácidos no códon 180 da molécula (Glu180Gly) e músculo papilar esquerdo de camundongos não – transgênicos (NTG), ambos nas idades de 2 à 3 semanas de vida e de 3 à 4 semanas de vida. Estes múscul...

  16. Imunodepressão induzida por talidomida e ciclosporina em transplante cardíaco heterotópico de coelho

    OpenAIRE

    Carvalho,João Batista Vieira de; Petroianu, Andy

    2003-01-01

    OBJETIVO: A talidomida, por seus efeitos antiinflamatórios e imunodepressores, tem sido utilizada no tratamento de doenças dermatológicas e na doença enxerto-contra-hospedeiro no transplante de medula óssea. O objetivo deste trabalho é avaliar a ação deste medicamento como imunodepressor em transplante de órgãos, estudando sua ação isoladamente ou em combinação com a ciclosporina na prevenção da rejeição ao aloenxerto cardíaco heterotópico em coelho. MÉTODO: Foram utilizados 50 coelhos, sendo...

  17. Variation morphogeometrics of Africanized honey bees (Apis mellifera in Brazil Variação morfogeométrica das abelhas africanizadas (Apis mellifera no Brasil

    Directory of Open Access Journals (Sweden)

    Lorena A. Nunes

    2012-09-01

    Full Text Available The morphometrics of the honey bee Apis mellifera L., 1758 has been widely studied mainly because this species has great ecological importance, high adaptation capacity, wide distribution and capacity to effectively adapt to different regions. The current study aimed to investigate the morphometric variations of wings and pollen baskets of honey bees Apis mellifera scutellata Lepeletier, 1836 from the five regions in Brazil. We used geometric morphometrics to identify the existence of patterns of variations of shape and size in Africanized honey bees in Brazil 16 years after the classic study with this species, allowing a temporal and spatial comparative analysis using new technological resources to assess morphometrical data. Samples were collected in 14 locations in Brazil, covering the five geographical regions of the country. The shape analysis and multivariate analyses of the wing allowed to observe that there is a geographical pattern among the population of Apis mellifera in Brazil. The geographical variations may be attributed to the large territorial extension of the country in addition to the differences between the bioregions.Apis mellifera L., 1758 têm sido alvo de muitos estudos morfométricos principalmente pela sua importância ecológica, pela sua grande capacidade de adaptação, sua ampla distribuição e por serem capazes de se estabelecer eficientemente em diversas regiões. O presente trabalho teve como objetivo estudar as variações da forma em asas e corbículas de operárias de Apis mellifera scutellata Lepeletier, 1836 provenientes das cinco regiões biogeográficas do Brasil utilizando análises morfogeométricas, a fim de verificar a existência de padrões de variação de forma e tamanho das abelhas africanizadas no Brasil após 16 anos do estudo clássico realizado com esta espécie, possibilitando uma análise espaço-temporal comparativa utilizando recursos tecnológicos atuais para a avaliação de dados morfom

  18. Cerebral physiology and preservation during cardiac arrest Fisiología y preservación cerebral durante el paro cardíaco: vulnerabilidad del cerebro

    Directory of Open Access Journals (Sweden)

    Luis M. Gómez

    1991-03-01

    Full Text Available

    Cerebral physiology during cardiac arrest is discussed with particular Interest on selective neuronal damage. Previous concepts on brain tolerance to hypoxia are analyzed and new information about brain function prognosis after cardiac arrest is presented. Therapeutic alternatives for brain preservation are discussed with emphasis on the lack of effectiveness of barbiturates, the results of research with other drugs and the future role that blockers of excitatory neurotransmission may have as elements of cerebral preservation.

    Se hace una aproximación a la fisiología cerebral durante el paro cardíaco, destacando el concepto de darlo neuronal selectivo. Se presentan elementos que permiten modificar concepciones antiguas sobre la tolerancia del cerebro a la hipoxia severa. Además, se hace un nuevo planteamiento sobre el pronóstico cerebral luego de un paro cardíaco y se esbozan las alternativas terapéuticas utilizadas hasta la fecha para la preservación cerebral. Se hace énfasis en la inefectividad de los barbitúricos, en la investigación sobre la terapia con otras drogas y en el futuro abierto hacia los bloqueadores de los neurotransmisores excitadores, como elementos terapéuticos para la preservación cerebral.

  19. A method for colocating satellite XCO2 data to ground-based data and its application to ACOS-GOSAT and TCCON

    Directory of Open Access Journals (Sweden)

    H. Nguyen

    2014-02-01

    Full Text Available Satellite measurements are often compared with higher-precision ground-based measurements as part of validation efforts. The satellite soundings are rarely perfectly coincident in space and time with the ground-based measurements, so a colocation methodology is needed to aggregate "nearby" soundings into what the instrument would have seen at the location and time of interest. We are particularly interested in validation efforts for satellite-retrieved total column carbon dioxide (XCO2, where XCO2 data from Greenhouse Gas Observing Satellite (GOSAT retrievals (ACOS, NIES, RemoteC, PPDF, etc. or SCanning Imaging Absorption SpectroMeter for Atmospheric CHartographY (SCHIACHY are often colocated and compared to ground-based column XCO2 measurement from Total Carbon Column Observing Network (TCCON. Current colocation methodologies for comparing satellite measurements of total column dry-air mole fractions of CO2 (XCO2 with ground-based measurements typically involve locating and averaging the satellite measurements within some latitudinal, longitudinal, and temporal window. We examine a geostatistical colocation methodology that takes a weighted average of satellite observations depending on the "distance" of each observation from a ground-based location of interest. The "distance" function that we use is a modified Euclidian distance with respect to latitude, longitude, time, and mid-tropospheric temperature at 700 hPa. We apply this methodology to XCO2 retrieved from Greenhouse Gas Observing Satellite (GOSAT spectra by the ACOS team, cross-validate the results to TCCON XCO2 ground-based data, and present some comparison between our methodology and standard existing colocation methods showing that in general geostatistical colocation produces smaller mean-squared error.

  20. A method for colocating satellite XCO2 data to ground-based data and its application to ACOS-GOSAT and TCCON

    Science.gov (United States)

    Nguyen, H.; Osterman, G.; Wunch, D.; O'Dell, C.; Mandrake, L.; Wennberg, P.; Fisher, B.; Castano, R.

    2014-08-01

    Satellite measurements are often compared with higher-precision ground-based measurements as part of validation efforts. The satellite soundings are rarely perfectly coincident in space and time with the ground-based measurements, so a colocation methodology is needed to aggregate "nearby" soundings into what the instrument would have seen at the location and time of interest. We are particularly interested in validation efforts for satellite-retrieved total column carbon dioxide (XCO2), where XCO2 data from Greenhouse Gas Observing Satellite (GOSAT) retrievals (ACOS, NIES, RemoteC, PPDF, etc.) or SCanning Imaging Absorption SpectroMeter for Atmospheric CHartographY (SCIAMACHY) are often colocated and compared to ground-based column XCO2 measurement from Total Carbon Column Observing Network (TCCON). Current colocation methodologies for comparing satellite measurements of total column dry-air mole fractions of CO2 (XCO2) with ground-based measurements typically involve locating and averaging the satellite measurements within a latitudinal, longitudinal, and temporal window. We examine a geostatistical colocation methodology that takes a weighted average of satellite observations depending on the "distance" of each observation from a ground-based location of interest. The "distance" function that we use is a modified Euclidian distance with respect to latitude, longitude, time, and midtropospheric temperature at 700 hPa. We apply this methodology to XCO2 retrieved from GOSAT spectra by the ACOS team, cross-validate the results to TCCON XCO2 ground-based data, and present some comparisons between our methodology and standard existing colocation methods showing that, in general, geostatistical colocation produces smaller mean-squared error.

  1. Vitellogenins are new high molecular weight components and allergens (Api m 12 and Ves v 6 of Apis mellifera and Vespula vulgaris venom.

    Directory of Open Access Journals (Sweden)

    Simon Blank

    Full Text Available BACKGROUND/OBJECTIVES: Anaphylaxis due to hymenoptera stings is one of the most severe clinical outcomes of IgE-mediated hypersensitivity reactions. Although allergic reactions to hymenoptera stings are often considered as a general model for the underlying principles of allergic disease, venom immunotherapy is still hampered by severe systemic side effects and incomplete protection. The identification and detailed characterization of all allergens of hymenoptera venoms might result in an improvement in this field and promote the detailed understanding of the allergological mechanism. Our aim was the identification and detailed immunochemical and allergological characterization of the low abundant IgE-reactive 200 kDa proteins of Apis mellifera and Vespula vulgaris venom. METHODS/PRINCIPAL FINDINGS: Tandem mass spectrometry-based sequencing of a 200 kDa venom protein yielded peptides that could be assigned to honeybee vitellogenin. The coding regions of the honeybee protein as well as of the homologue from yellow jacket venom were cloned from venom gland cDNA. The newly identified 200 kDa proteins share a sequence identity on protein level of 40% and belong to the family of vitellogenins, present in all oviparous animals, and are the first vitellogenins identified as components of venom. Both vitellogenins could be recombinantly produced as soluble proteins in insect cells and assessed for their specific IgE reactivity. The particular vitellogenins were recognized by approximately 40% of sera of venom-allergic patients even in the absence of cross-reactive carbohydrate determinants. CONCLUSION: With the vitellogenins of Apis mellifera and Vespula vulgaris venom a new homologous pair of venom allergens was identified and becomes available for future applications. Due to their allergenic properties the honeybee and the yellow jacket venom vitellogenin were designated as allergens Api m 12 and Ves v 6, respectively.

  2. OpenSearch (ECHO-ESIP) & REST API for Earth Science Data Access

    Science.gov (United States)

    Mitchell, A.; Cechini, M.; Pilone, D.

    2010-12-01

    This presentation will provide a brief technical overview of OpenSearch, the Earth Science Information Partners (ESIP) Federated Search framework, and the REST architecture; discuss NASA’s Earth Observing System (EOS) ClearingHOuse’s (ECHO) implementation lessons learned; and demonstrate the simplified usage of these technologies. SOAP, as a framework for web service communication has numerous advantages for Enterprise applications and Java/C# type programming languages. As a technical solution, SOAP has been a reliable framework on top of which many applications have been successfully developed and deployed. However, as interest grows for quick development cycles and more intriguing “mashups,” the SOAP API loses its appeal. Lightweight and simple are the vogue characteristics that are sought after. Enter the REST API architecture and OpenSearch format. Both of these items provide a new path for application development addressing some of the issues unresolved by SOAP. ECHO has made available all of its discovery, order submission, and data management services through a publicly accessible SOAP API. This interface is utilized by a variety of ECHO client and data partners to provide valuable capabilities to end users. As ECHO interacted with current and potential partners looking to develop Earth Science tools utilizing ECHO, it became apparent that the development overhead required to interact with the SOAP API was a growing barrier to entry. ECHO acknowledged the technical issues that were being uncovered by its partner community and chose to provide two new interfaces for interacting with the ECHO metadata catalog. The first interface is built upon the OpenSearch format and ESIP Federated Search framework. Leveraging these two items, a client (ECHO-ESIP) was developed with a focus on simplified searching and results presentation. The second interface is built upon the Representational State Transfer (REST) architecture. Leveraging the REST architecture, a

  3. Polinização de morangueiro por Apis mellifera em ambiente protegido Strawberry polinization through Apis mellifera in protected environment

    Directory of Open Access Journals (Sweden)

    Eunice Oliveira Calvete

    2010-03-01

    Full Text Available Este trabalho objetivou comparar a produtividade e a qualidade dos frutos comerciáveis no morangueiro, em ambiente protegido, utilizando a espécie de abelha Apis mellifera em relação à ausência de um agente polinizador. Foram instalados dois experimentos, utilizando-se das cultivares Camarosa, Oso Grande, Diamante e Aromas (tratamentos, no período de 05 de maio a 19 de dezembro de 2006. No primeiro experimento, não havia polinizadores (testemunha. O segundo foi polinizado pela espécie de abelha Apis mellifera. Os tratamentos (cultivares foram dispostos, em cada experimento, em um delineamento completamente casualizado, com seis repetições. Foram avaliadas características de rendimento, bem como análise físico-química dos frutos de cada cultivar. Quanto ao rendimento, a maior produção obtida foi com a cultivar Camarosa tanto na ausência quanto na presença de polinizadores (907,46 g. planta-1 e 1.054,93 g. planta-1, respectivamente, ao passo que a cultivar Oso Grande apresentou menor número de frutos deformados.This work objectified to compare the productivity and the quality of the strawberry marketable fruits in protected environment, using the species of bee Apis mellifera in relation of the absence of a polinizator agent. It was installed two experiments using the cultivates Camarosa, Oso Grande, Diamante and Aromas (treatments, in the period of May 5 th until December 19th of 2006. The first experiment did not have polinizatores agents (control. The second was polinizated by the species of bee Apis mellifera. The treatments (cultivars had been arranged, in each experiment in a randomized blocks with 6 repetitions. They were evaluated income characteristics, as well as analysis physicist-chemistry of the fruits of each cultivar. For the income, the biggest production was gotten in cultivar Camarosa, for the first and the second experiment (907,46 g. plant-1 and 1054,93 g. plant-1, respectively, in the way that the cultivate

  4. Transplante cardíaco ortotópico: experiência na Universidade Federal de São Paulo

    Directory of Open Access Journals (Sweden)

    João Nelson Rodrigues BRANCO

    1998-10-01

    Full Text Available Foram realizados no período de novembro de 1986 a abril de 1997, 92 transplantes ortotópicos, com receptores na faixa etária de 3 a 63 anos (média de 44,9 anos. Os diagnósticos pré-operatórios foram de miocardiopatia dilatada em 42 (44,6% pacientes, isquêmica em 23 (25,0%, chagásica em 21 (22,8%, valvar em 3 (3,2% e outras miocardiopatias em 3 (3,2% pacientes. A técnica operatória empregada (Lower e Shumway, em 1960 foi satisfatória e sem complicações. O tempo de isquemia - mais longo nos transplantes com captação à distância - foi sempre inferior a quatro horas. As complicações relacionadas ao uso crônico de imunossupressores foram, principalmente, hipertensão arterial (84,6%, hiperuricemia (75,4% e dislipidemia (63,0%. Quanto às infecções, houve predomínio das virais, com 42 (45,6% casos, seguidas das bacterianas, com 35 (38,0% casos, e das por protozoários, com 15 (16,3% casos. Dentre as infecções bacterianas, sete foram do sítio cirúrgico, com boa evolução. Das infecções por protozoários, sete (46,6% forma por reativação do Trypanosoma cruzi. A mortalidade geral nos primeiros 30 dias do pós-operatório foi de 17,3% e teve, como principais causas: infecção, complicações neurológicas e rejeição. Após esses 30 dias e até o primeiro ano, houve 10 (10,3% óbitos, com predomínio de rejeição e infecção como causas. Após o primeiro ano de pós-operatório, houve 13 (14,0% falecimentos, por causas diversas, como: morte súbita, infecção, rejeição, além de outras. A curva actuarial mostrou no 1º, 2º, 3º, 4º, 5º e 6º anos uma sobrevivência de receptores de 71,6%, 66,6%, 60,5%, 54,4%, 54,4%, 54,4%, respectivamente. Não houve perda de seguimento de paciente, e os sobreviventes até a conclusão do trabalho encontravam-se bem, todos em classe funcional I da NYHA. Os autores concluem que é possível a realização de transplante cardíaco em nossa comunidade com sobrevivência e taxa de

  5. Development of a GIS-Based Online Discussion System for Scientists with Google Earth API and Twitter

    Science.gov (United States)

    Ohtake, S.; Demura, H.; Hirata, N.; Terazono, J.

    2011-03-01

    This study has developed an online discussion system cooperating with GIS for researchers in planetary sciences. We focused on mushup approach with the existing web services; Google Earth API and Twitter.

  6. The indigenous honey bees of Saudi Arabia (Hymenoptera, Apidae, Apis mellifera jemenitica Ruttner): Their natural history and role in beekeeping.

    Science.gov (United States)

    Alqarni, Abdulaziz S; Hannan, Mohammed A; Owayss, Ayman A; Engel, Michael S

    2011-01-01

    Apis mellifera jemenitica Ruttner (= yemenitica auctorum: videEngel 1999) has been used in apiculture throughout the Arabian Peninsula since at least 2000 BC. Existing literature demonstrates that these populations are well adapted for the harsh extremes of the region. Populations of Apis mellifera jemenitica native to Saudi Arabia are far more heat tolerant than the standard races often imported from Europe. Central Saudi Arabia has the highest summer temperatures for the Arabian Peninsula, and it is in this region where only Apis mellifera jemenitica survives, while other subspecies fail to persist. The indigenous race of Saudi Arabia differs from other subspecies in the region in some morphological, biological, and behavioral characteristics. Further taxonomic investigation, as well as molecular studies, is needed in order to confirm whether the Saudi indigenous bee populations represent a race distinct from Apis mellifera jemenitica, or merely an ecotype of this subspecies.

  7. PERLETAKAN STASIUN KERETA API DALAM TATA RUANG KOTA-KOTA DI JAWA (KHUSUSNYA JAWA TIMUR PADA MASA KOLONIAL

    Directory of Open Access Journals (Sweden)

    Handinoto Handinoto

    1999-01-01

    Full Text Available Railway Company in Indonesia started in 1860's. They were held by both government (SS- Staad Spoorwegen and private (NIS, etc. The same happened as in Europe after rev. industry, railway station's placement as new kind of building became very important in urban planning. Faster progression,in railway services in Indonesia in the begining 20 th century, that reached almost all of town in Java; caused railway station's placement, either in larger city or Kabupaten city will be importance. In the end of 19 th and 20 th century, railway transportation was one of important infrastructure.But in the second part of 20 th century, after independence, the roadway progression caused railway services become come down, so railway stations were careless. In the end of 20 th century, the dense of roadway in Java caused railway's function raise again. In general towns has been develop, so railway station placement which been though exactly in urban planning, become to make trouble for city trafic. The scoupe of this paper covered about the placement of railway station in the past, as input for development city in Java for the future. Abstract in Bahasa Indonesia : Per kereta api an di Indonesia baru dimulai pada th. 1860 an. Perusahaan kereta api ditangani oleh dua instansi yaitu oleh pihak pemerintah (seperti: S.S - Staad Spoorwegen dan pihak swasta (seperti :NIS - Nederlandsch Indische Spoorweg Maatschappij, dan sebagainya. Seperti halnya di Eropa setelah revolusi industri, perletakkan stasiun sebagai suatu jenis bangunan baru, menjadi sangat penting dalam tata ruang kota. Dengan makin majunya per kereta api an di Indonesia pada awal abad ke 20, yang hampir mencapai seluruh kota di Jawa, maka penempatan stasiun kereta api baik di kota-kota besar maupun kota Kabupaten menjadi suatu pemikiran yang penting. Pada akhir abad ke 19 dan abad ke 20, angkutan dengan kereta api, menjadi salah satu sarana yang sangat penting, baik angkutan barang maupun manusia. Tapi

  8. The candidate genes TAF5L, TCF7, PDCD1, IL6 and ICAM1 cannot be excluded from having effects in type 1 diabetes

    Directory of Open Access Journals (Sweden)

    Vella Adrian

    2007-11-01

    Full Text Available Abstract Background As genes associated with immune-mediated diseases have an increased prior probability of being associated with other immune-mediated diseases, we tested three such genes, IL23R, IRF5 and CD40, for an association with type 1 diabetes. In addition, we tested seven genes, TAF5L, PDCD1, TCF7, IL12B, IL6, ICAM1 and TBX21, with published marginal or inconsistent evidence of an association with type 1 diabetes. Methods We genotyped reported polymorphisms of the ten genes, nonsynonymous SNPs (nsSNPs and, for the IL12B and IL6 regions, tag SNPs in up to 7,888 case, 8,858 control and 3,142 parent-child trio samples. In addition, we analysed data from the Wellcome Trust Case Control Consortium genome-wide association study to determine whether there was any further evidence of an association in each gene region. Results We found some evidence of associations between type 1 diabetes and TAF5L, PDCD1, TCF7 and IL6 (ORs = 1.05 – 1.13; P = 0.0291 – 4.16 × 10-4. No evidence of an association was obtained for IL12B, IRF5, IL23R, ICAM1, TBX21 and CD40, although there was some evidence of an association (OR = 1.10; P = 0.0257 from the genome-wide association study for the ICAM1 region. Conclusion We failed to exclude the possibility of some effect in type 1 diabetes for TAF5L, PDCD1, TCF7, IL6 and ICAM1. Additional studies, of these and other candidate genes, employing much larger sample sizes and analysis of additional polymorphisms in each gene and its flanking region will be required to ascertain their contributions to type 1 diabetes susceptibility.

  9. Analysis of API2-MALT1 fusion, trisomies, and immunoglobulin VH genes in pulmonary mucosa-associated lymphoid tissue lymphoma.

    Science.gov (United States)

    Xia, Hongjing; Nakayama, Takahisa; Sakuma, Hidenori; Yamada, Seiji; Sato, Fumihiko; Takino, Hisashi; Okabe, Mitsukuni; Fujiyoshi, Yukio; Hattori, Hideo; Inagaki, Hiroshi

    2011-09-01

    Pulmonary mucosa-associated lymphoid tissue lymphoma is unique in that chronic inflammation is rare and that API2-MALT1 fusion, resulting from t(11;18)(q21;q21), occurs frequently. In this study, we examined 20 cases for API2-MALT1 fusion using the multiplex reverse-transcription polymerase chain reaction and looked for trisomy 3, trisomy 18, and abnormalities of MALT1 and IGH genes using fluorescence in situ hybridization. In addition, we analyzed VH genes by subcloning of the monoclonal polymerase chain reaction products. Of 20 cases studied, we detected gene abnormalities in 16: API2-MALT1 fusion in 9, trisomy 3 in 5, trisomy 18 in 4, MALT1 abnormality in 13, and IGH abnormality in 1. MALT1 gene abnormalities were concordant with API2-MALT1 fusion or trisomy 18. One case showed API2-MALT1 fusion and trisomy 3. On detection of API2-MALT1 fusion and trisomies, we were able to divide our cases into 3 groups, API2-MALT1 positive, trisomy positive, and no detectable gene abnormality, suggesting that tumor development had processed along different genetic pathways. All 20 cases were analyzed for VH genes. Most of the VH genes selected by the lymphomas belonged to the VH3 family, but there was no restriction to any particular VH fragment. Of interest, VH genes were unmutated in 7 cases, suggesting that T-cell-independent extrafollicular B-cell maturation may be important in the development of this lymphoma. In addition, both mutated and unmutated tumor cases were found to carry the API2-MALT1 fusion and trisomy 3. This observation suggests that these gene abnormalities may occur in microenvironments found before or outside of follicular germinal centers.

  10. 环境空气中臭氧API评价探讨%Study on Air Ozone API Evaluation

    Institute of Scientific and Technical Information of China (English)

    徐建平

    2012-01-01

    Air ozone API evaluation is considered that area of influence on ozone concentration exceed ambient air quality, ozone API correction on hours on ozone concentration exceed ambient air quality and Ozone average concentration exceed ambient air quality.%环境空气中臭氧API评价应考虑臭氧超标影响范围、臭氧超标小时数和超标平均浓度对臭氧API修正.

  11. Test-retest reliability of the Advanced Psychodiagnostic Interpretation (API) scoring system for the Bender Gestalt in chronic schizophrenics.

    Science.gov (United States)

    Aucone, E J; Wagner, E E; Raphael, A J; Golden, C J; Espe-Pfeifer, P; Dornheim, L; Seldon, J; Pospisil, T; Proctor-Weber, Z; Calabria, M

    2001-09-01

    This study assesses the test-retest reliability of the revised Advanced Psychodiagnostic Interpretation (API) scoring system for the Bender Gestalt Test (BGT). The API system identifies 207 possible distortions in a BGT protocol. Test-retest reliability for 40 schizophrenic patients tested twice with a mean interval of 6.4 years (SD=3.8 years) was good, ranging from .71 to .80. Further reliability and validity studies are needed to further demonstrate the effectiveness of the system.

  12. OWL应用接口的比较分析%Comparative Analysis of APIs for OWL Ontology

    Institute of Scientific and Technical Information of China (English)

    熊振辉; 于娟

    2015-01-01

    The paper comparatively analyzes three types of APIs for OWL, including Jena API、Protégé API and OWL API, from aspects of characteristics, platforms which they base on, languages which they can process and OWL ontology models. It focuses on commonly-used functions of OWL API and obtains related conclusions: (1) Jena API cannot achieve all functions of OWL because it bases on RDF triple;(2) OWL API is specifically designed for OWL and it supports structural specification of OWL2 which is the latest edition of OWL;(3) current APIs for OWL ontology mostly base on Java platform, whereas APIs base on other platforms (e.g. .net plat-form) have not appeared.%比较分析了Jena API、Protégé API、OWL API这三种OWL应用接口(API)的特点、所基于的平台、可处理的语言以及描述OWL本体的模型,重点介绍了OWL API的常用基本功能,得出了相关结论:(1)Jena API基于RDF三元组的特点使其不能实现OWL语言的全部功能;(2)OWL API针对OWL语言设计,且支持最新的OWL2语言的结构规范;(3)目前这些API都是基于Java平台的,对于其它平台(如.net平台),尚未见有成熟的API出现。

  13. Evaluation of different glycoforms of honeybee venom major allergen phospholipase A2 (Api m 1) produced in insect cells

    DEFF Research Database (Denmark)

    Blank, Simon; Seismann, Henning; Plum, Melanie;

    2011-01-01

    Allergic reactions to hymenoptera stings are one of the major reasons for IgE-mediated anaphylaxis. However, proper diagnosis using venom extracts is severely affected by molecular cross-reactivity. In this study recombinant honeybee venom major allergen phospholipase A2 (Api m 1) was produced......-derived recombinant Api m 1 with defined CCD phenotypes might provide further insights into hymenoptera venom IgE reactivities and contribute to an improved diagnosis of hymenoptera venom allergy....

  14. The API2-MALT1 fusion exploits TNFR pathway-associated RIP1 ubiquitination to promote oncogenic NF-κB signaling.

    Science.gov (United States)

    Rosebeck, S; Rehman, A O; Apel, I J; Kohrt, D; Appert, A; O'Donnell, M A; Ting, A T; Du, M-Q; Baens, M; Lucas, P C; McAllister-Lucas, L M

    2014-05-08

    The API2-MALT1 fusion oncoprotein is created by the recurrent t(11;18)(q21;q21) chromosomal translocation in mucosa-associated lymphoid tissue (MALT) lymphoma. We identified receptor interacting protein-1 (RIP1) as a novel API2-MALT1-associated protein, and demonstrate that RIP1 is required for API2-MALT1 to stimulate canonical nuclear factor kappa B (NF-κB). API2-MALT1 promotes ubiquitination of RIP1 at lysine (K) 377, which is necessary for full NF-κB activation. Furthermore, we found that TNF receptor-associated factor 2 (TRAF2) recruitment is required for API2-MALT1 to induce RIP1 ubiquitination, NF-κB activation and cellular transformation. Although both TRAF2 and RIP1 interact with the API2 moiety of API2-MALT1, this moiety alone is insufficient to induce RIP1 ubiquitination or activate NF-κB, indicating that API2-MALT1-dependent RIP1 ubiquitination represents a gain of function requiring the concerted actions of both the API2 and MALT1 moieties of the fusion. Intriguingly, constitutive RIP1 ubiquitination was recently demonstrated in several solid tumors, and now our study implicates RIP1 ubiquitination as a critical component of API2-MALT1-dependent lymphomagenesis.

  15. Perfil dos pacientes na Lista Única de Espera para transplante cardíaco no estado do Ceará Perfil de los pacientes en la lista única de espera para transplante cardíaco en el estado de Ceará Profile of patients in the Unified Waiting List for heart transplantation in state of Ceará

    Directory of Open Access Journals (Sweden)

    Francisca Elisângela Teixeira Lima

    2010-07-01

    Full Text Available FUNDAMENTO: Os transplantes de órgãos têm aumentado consideravelmente nos últimos anos em razão da evolução tecnológica e da sensibilização da sociedade para doação de órgãos. OBJETIVO: Descrever as características dos pacientes da Lista Única de Espera para transplante cardíaco; identificar as principais cardiopatias; e verificar o tempo médio de permanência do paciente na Lista até a realização da cirurgia. MÉTODOS: Trata-se de um estudo descritivo, documental e retrospectivo, com abordagem quantitativa, desenvolvido na Central de Transplante do Estado do Ceará, com 156 pacientes incluídos na Lista Única de Espera do ano de 1999 a 2006. Os dados foram organizados em figuras. RESULTADOS: Foram encontrados: 81% do sexo masculino; 22,4% adultos jovens (20 a 40 anos e 56,4% adultos de meia-idade (40 a 64 anos, com uma média de 36 anos; 79% procedentes de Fortaleza-CE; 91% tinham miocardiopatia como causa do transplante cardíaco. Dentre esses pacientes 102 (69% foram transplantados; 37 (25% evoluíram para óbito antes do transplante e 8 (6% foram excluídos por melhora ou piora do quadro clínico. CONCLUSÃO: Os pacientes da Lista Única de Espera para transplante cardíaco no Estado do Ceará, no período de 1999 a 2006, eram do sexo masculino (80%, com faixa etária variando de 1 a 71 anos, com predomínio da miocardiopatia dilatada (53,4%, e o tempo médio de espera foi de 136 dias até o dia do transplante cardíaco.FUNDAMENTO: Los transplantes de órganos han aumentado considerablemente en los últimos años, en razón de la evolución tecnológica y de la sensibilización de la sociedad para la donación de órganos. OBJETIVO: Describir las características de los pacientes de la Lista Única de Espera para transplante cardíaco; identificar las principales cardiopatías; y verificar el tiempo medio de permanencia del paciente en la Lista hasta la realización de la cirugía. MÉTODOS: Se trata de un estudio

  16. Sedação com sufentanil e clonidina em pacientes submetidos a cateterismo cardíaco Sedación con sufentanil y clonidina en pacientes sometidos a cateterismo cardíaco Sedation with sufentanil and clonidine in patients undergoing heart catheterization

    Directory of Open Access Journals (Sweden)

    Anita Perpetua Carvalho Rocha

    2011-03-01

    Full Text Available FUNDAMENTO: A sedação para a realização de cateterismo cardíaco tem sido alvo de preocupação. Benzodiazepínicos, agonistas alfa-2 adrenérgicos e opioides são utilizados para esse fim, entretanto, cada um destes medicamentos possui vantagens e desvantagens. OBJETIVO: Avaliar a eficácia do sufentanil e da clonidina como sedativos em pacientes submetidos a cateterismo cardíaco, observando o impacto dos mesmos sobre os parâmetros hemodinâmicos e respiratórios, a presença de efeitos colaterais, além da satisfação do paciente e do hemodinamicista com o exame. MÉTODOS: Trata-se de um ensaio clínico prospectivo, duplo-cego, randomizado e controlado, que envolveu 60 pacientes que receberam 0,1 µg/kg de sufentanil ou 0,5 µg/kg de clonidina antes da realização do cateterismo cardíaco. O escore de sedação segundo a escala de Ramsay, a necessidade de utilização de midazolam, os efeitos colaterais, os parâmetros hemodinâmicos e respiratórios foram registrados, sendo os dados analisados em 06 diferentes momentos. RESULTADOS: O comportamento da pressão arterial, da frequência cardíaca e da frequência respiratória foi semelhante nos dois grupos, entretanto, no momento 2, os pacientes do grupo sufentanil (Grupo S apresentaram menor escore de sedação segundo a escala de Ramsay, e a saturação periférica da oxihemoglobina foi menor que o grupo clonidina (Grupo C no momento 6. Os pacientes do Grupo S apresentaram maior incidência de náusea e vômito pós-operatório que os pacientes do Grupo C. A satisfação dos pacientes foi maior no grupo clonidina. Os hemodinamicistas mostraram-se satisfeitos nos dois grupos. CONCLUSÃO: O sufentanil e a clonidina foram efetivos como sedativos em pacientes submetidos a cateterismo cardíaco.FUNDAMENTO: La sedación para la realización de cateterismo cardíaco ha sido blanco de preocupación. Benzodiazepínicos, agonistas alfa-2 adrenérgicos y opioides son utilizados para ese fin

  17. Comparative proteomic analysis reveals mite (Varroa destructor) resistance-related proteins in Eastern honeybees (Apis cerana).

    Science.gov (United States)

    Ji, T; Shen, F; Liu, Z; Yin, L; Shen, J; Liang, Q; Luo, Y X

    2015-08-21

    The mite (Varroa destructor) has become the greatest threat to apiculture worldwide. As the original host of the mite, Apis cerana can effectively resist the mite. An increased understanding of the resistance mechanisms of Eastern honeybees against V. destructor may help researchers to protect other species against these parasites. In this study, the proteomes of 4 Apis cerana colonies were analyzed using an isobaric tag for relative and absolute quantitation technology. We determined the differences in gene and protein expression between susceptible and resistant colonies that were either unchallenged or challenged by V. destructor. The results showed that a total of 1532 proteins were identified. Gene Ontology enrichment analysis suggested that the transcription factors and basic metabolic and respiratory processes were efficient and feasible factors controlling this resistance, and 12 differentially expressed proteins were identified in Venn analysis. The results were validated by quantitative polymerase chain reaction. This study may provide insight into the genetic mechanisms underlying the resistance of honeybee to mites.

  18. Developing a Digital Video Library with the YouTube Data API

    Directory of Open Access Journals (Sweden)

    Jason Clark

    2013-04-01

    Full Text Available MSU Library has created a digital video library using the YouTube API to power our local library channel. It is a complete search and browse application with item level views, microdata, a caching and optimization routine, and a file backup routine. The article will discuss applying the YouTube API as a database application layer: workflow efficiencies, metadata procedures and local backup and optimization procedures. Code samples in PHP, .htaccess examples, and shell commands used in developing the application and routines will be explained at length. And finally, a complete prototype application will be released on github for other libraries to get started using the lessons learned. A live version of the application is here: http://www.lib.montana.edu/channel/. The real benefit of this method is the low overhead for smaller shops and the ability to scale production and distribution of digital video.

  19. Temporal genetic structure of a drone congregation area of the giant Asian honeybee (Apis dorsata).

    Science.gov (United States)

    Kraus, F B; Koeniger, N; Tingek, S; Moritz, R F A

    2005-12-01

    The giant Asian honeybee (Apis dorsata), like all other members of the genus Apis, has a complex mating system in which the queens and males (drones) mate at spatially defined drone congregation areas (DCAs). Here, we studied the temporal genetic structure of a DCA of A. dorsata over an 8-day time window by the genotyping of sampled drones with microsatellite markers. Analysis of the genotypic data revealed a significant genetic differentiation between 3 sampling days and indicated that the DCA was used by at least two subpopulations at all days in varying proportions. The estimation of the number of colonies which used the DCA ranged between 20 and 40 colonies per subpopulation, depending on the estimation procedure and population. The overall effective population size was estimated as high as N (e)=140. The DCA seems to counteract known tendencies of A. dorsata for inbreeding within colony aggregations by facilitating gene flow among subpopulations and increasing the effective population size.

  20. Opening the Door: A First Look at the OCLC WorldCat Metadata API

    Directory of Open Access Journals (Sweden)

    Terry Reese

    2014-07-01

    Full Text Available Libraries have long relied on OCLC’s WorldCat database as a way to cooperatively share bibliographic data and declare library holdings to support interlibrary loan services. As curator, OCLC has traditionally mediated all interactions with the WorldCat database through their various cataloging clients to control access to the information. As more and more libraries look for new ways to interact with their data and streamline metadata operations and workflows, these clients have become bottlenecks and an inhibitor of library innovation. To address some of these concerns, in early 2013 OCLC announced the release of a set of application programming interfaces (APIs supporting read and write access to the WorldCat database. These APIs offer libraries their first opportunity to develop new services and workflows that directly interact with the WorldCat database, and provide opportunities for catalogers to begin redefining how they work with OCLC and their data.

  1. TogoWS: integrated SOAP and REST APIs for interoperable bioinformatics Web services.

    Science.gov (United States)

    Katayama, Toshiaki; Nakao, Mitsuteru; Takagi, Toshihisa

    2010-07-01

    Web services have become widely used in bioinformatics analysis, but there exist incompatibilities in interfaces and data types, which prevent users from making full use of a combination of these services. Therefore, we have developed the TogoWS service to provide an integrated interface with advanced features. In the TogoWS REST (REpresentative State Transfer) API (application programming interface), we introduce a unified access method for major database resources through intuitive URIs that can be used to search, retrieve, parse and convert the database entries. The TogoWS SOAP API resolves compatibility issues found on the server and client-side SOAP implementations. The TogoWS service is freely available at: http://togows.dbcls.jp/.

  2. Beginning the 21st century with advanced Automatic Parts Identification (API)

    Science.gov (United States)

    Schramm, Fred; Roxby, Don

    1994-01-01

    Under the direction of the NASA George C. Marshall Space Flight Center, Huntsville, Alabama, the development and commercialization of an advanced Automated Parts Indentification (API) system is being undertaken by Rockwell International Corporation. The new API system is based on a variable sized, machine-readable, two-dimensioanl matrix symbol that can be applied directly onto most metallic and nonmetallic materials using safe, permanent marking methods. Its checkerboard-like structure is the most space efficient of all symbologies. This high data-density symbology can be applied to products of different material sizes and geometries using application-dependent, computer-driven marking devices. The high fidelity markings produced by these devices can then be captured using a specially designed camera linked to any IBM-compatible computer. Applications of compressed symbology technology will reduce costs and improve quality, productivity, and processes in a wide variety of federal and commercial applications.

  3. Genetic reincarnation of workers as queens in the Eastern honeybee Apis cerana.

    Science.gov (United States)

    Holmes, M J; Tan, K; Wang, Z; Oldroyd, B P; Beekman, M

    2015-01-01

    Thelytokous parthenogenesis, or the asexual production of female offspring, is rare in the animal kingdom, but relatively common in social Hymenoptera. However, in honeybees, it is only known to be ubiquitous in one subspecies of Apis mellifera, the Cape honeybee, A. mellifera capensis. Here we report the appearance of queen cells in two colonies of the Eastern honeybee Apis cerana that no longer contained a queen or queen-produced brood to rear queens from. A combination of microsatellite genotyping and the timing of the appearance of these individuals excluded the possibility that they had been laid by the original queen. Based on the genotypes of these individuals, thelytokous production by natal workers is the most parsimonious explanation for their existence. Thus, we present the first example of thelytoky in a honeybee outside A. mellifera. We discuss the evolutionary and ecological consequences of thelytoky in A. cerana, in particular the role thelytoky may play in the recent invasions by populations of this species.

  4. Prevalence of nursing diagnosis of decreased cardiac output and the predictive value of defining characteristics in patients under evaluation for heart transplant Prevalencia del diagnóstico de enfermería de disminución del gasto cardíaco y valor predictivo de las características definidoras en pacientes en fase de evaluación para trasplante cardíaco Prevalência do diagnóstico de enfermagem de débito cardíaco diminuído e valor preditivo das características definidoras em pacientes em avaliação para transplante cardíaco

    Directory of Open Access Journals (Sweden)

    Lígia Neres Matos

    2012-04-01

    Full Text Available The purposes of the study were to identify the prevalence of defining characteristics (DC of decreased cardiac output (DCO in patients with cardiac insufficiency under evaluation for heart transplantation, and to ascertain the likelihood of defining characteristics being predictive factors for the existence of reduction in cardiac output. Data was obtained by retrospective documental analysis of the clinical records of right-sided heart catheterizations in 38 patients between 2004 and 2009. The results showed that 71.1% of the patients had decreased cardiac output (measured by cardiac index. The majority of the NANDA-International defining characteristics for DCO were more frequent in individuals with reduced cardiac index levels. The study emphasizes the odds ratio (OR for increased Systemic Vascular Resistance of OR=4.533, of the third heart sound with OR=3.429 and the reduced ejection fraction with OR=2.850. By obtaining the predictive values for the defining characteristics the study identifies them as diagnostic indicators of decreased cardiac output.El estudio tiene como objetivos identificar la prevalencia de las características definitorias de la disminución del gasto cardíaco (DGC en pacientes con insuficiencia cardíaca (IC en evaluación para el trasplante de corazón y ver la probabilidad de las características definitorias sean factores predictivos de la existencia de una disminución del gasto cardíaco. Los datos se obtuvieron mediante el análisis documental retrospectivo de historias clínicas de cateterismo cordiaco derecho en 38 pacientes con insuficiencia cardíaca en la evaluación para el trasplante de corazón entre 2004 y 2009. Los resultados mostraron que el 71% de los pacientes habían reducido el gasto cardíaco (medido por el índice cardíaco. La mayoría de las características definitorias de la NANDA-International para el DGC fueron más frecuentes en individuos con índice cardíaco reducido. Los aspectos m

  5. csd alleles in the red dwarf honey bee (Apis florea,Hymenoptera: Apidae) show exceptionally high nucleotide diversity

    Institute of Scientific and Technical Information of China (English)

    Zhi-Yong Liu; Zi-Long Wang; Xiao-Bo Wu; Wei-Yu Yan; Zhi-Jiang Zeng

    2011-01-01

    The single locus complementary sex determination (sl-csd) gene is the primary gene determining the gender of honey bees (Apis spp.).While the csd gene has been well studied in the Western honey bee (Apis mellifera),and comparable data exist in both the Eastern honey bee (Apis cerana) and the giant honey bee (Apis dorsata),no studies have been conducted in the red dwarf honey bee,Apisflorea.In this study we cloned the genomic region 3 of the A.florea csd gene from 60 workers,and identified 12 csd alleles.Analysis showed that similar to A.mellifera,region 3 of the csd gene contains a RS domain at the N terminal,a proline-rich domain at the C terminal,and a hypervariable region in the middle.However,the A.florea csd gene possessed a much higher level of nucleotide diversity,compared to A.mellifera,A.cerana and Apis dorsata.We also show that similar to the other three Apis species,in A.florea,nonsynonymous mutations in the csd gene are selectively favored in young alleles.

  6. Novel insights into excipient effects on the biopharmaceutics of APIs from different BCS classes: Lactose in solid oral dosage forms.

    Science.gov (United States)

    Kubbinga, Marlies; Moghani, Laura; Langguth, Peter

    2014-09-30

    Excipients encompass a wide range of properties that are of importance for the resulting drug product. Regulatory guidelines on biowaivers for immediate release formulations require an in depth understanding of the biopharmaceutic effects of excipients in order to establish bioequivalence between two different products carrying the same API based on dissolution tests alone. This paper describes a new approach in evaluating biopharmaceutic excipient effects. Actually used quantities of a model excipient, lactose, formulated in combination with APIs from different BCS classes were evaluated. The results suggest that companies use different (relative) amounts depending on the characteristics of the API. The probability of bioinequivalence due to a difference in lactose content between test and reference products was classified as low for BCS class I APIs and medium for BCS class II and III APIs, whereas a high probability was assigned to the combination of lactose and BCS class IV APIs. If repeated for other excipients, this retrospective, top-down approach may lead to a new database and more widespread applications of the biowaiver approach.

  7. Dry coating of micronized API powders for improved dissolution of directly compacted tablets with high drug loading.

    Science.gov (United States)

    Han, Xi; Ghoroi, Chinmay; Davé, Rajesh

    2013-02-14

    Motivated by our recent study showing improved flow and dissolution rate of the active pharmaceutical ingredient (API) powders (20 μm) produced via simultaneous micronization and surface modification through continuous fluid energy milling (FEM) process, the performance of blends and direct compacted tablets with high drug loading is examined. Performance of 50 μm API powders dry coated without micronization is also considered for comparison. Blends of micronized, non-micronized, dry coated or uncoated API powders at 30, 60 and 70% drug loading, are examined. The results show that the blends containing dry coated API powders, even micronized ones, have excellent flowability and high bulk density compared to the blends containing uncoated API, which are required for direct compaction. As the drug loading increases, the difference between dry coated and uncoated blends is more pronounced, as seen in the proposed bulk density-FFC phase map. Dry coating led to improved tablet compactibility profiles, corresponding with the improvements in blend compressibility. The most significant advantage is in tablet dissolution where for all drug loadings, the t(80) for the tablets with dry coated APIs was well under 5 min, indicating that this approach can produce nearly instant release direct compacted tablets at high drug loadings.

  8. Efeitos de atrativos e repelentes sobre o comportamento da abelha (Apis mellifera, l. Effect of attractants and repellents on the behavior of honey bees (Apis mellipera, L.

    Directory of Open Access Journals (Sweden)

    D.T. MALERBO-SOUZA

    1998-01-01

    Full Text Available O objetivo do presente ensaio foi testar diversas substâncias e verificar sua atratividade e repelência para abelhas, Apis mellifera, L. Para isso, foram realizados testes em discos próximos às colméias e em cana picada oferecida como alimentação para gado bovino confinado. Nos discos próximos à colméia, o produto mais atrativo foi Bee-Here e o mais repelente foi o óleo de citronela. No cocho para bovinos, o repelente mais efetivo foi n-octyl-acetato.The experiment aimed to study honey bee (Apis mellifera attractants and repellents in vitro and on chopped sugar cane for bovine feeding. Tests were performed on plates located near to the hives and on bovine-hods. On plates, Bee-Here® was the most attractive and citronela oil the most repellent. On bovine-hods the most effective was n-octyl-acetate.

  9. Protease activity of the API2-MALT1 fusion oncoprotein in MALT lymphoma development and treatment.

    Science.gov (United States)

    Rosebeck, Shaun; Lucas, Peter C; McAllister-Lucas, Linda M

    2011-05-01

    Gastric mucosa-associated lymphoid tissue (MALT) lymphoma is a prototypical cancer that occurs in the setting of chronic inflammation and an important model for understanding how deregulated NF-κB transcriptional activity contributes to malignancy. Most gastric MALT lymphomas require ongoing antigenic stimulation for continued tumor growth, and Stage I disease is usually cured by eradicating the causative microorganism, Helicobacter pylori, with antibiotics. However, in a subset of MALT lymphomas, chromosomal translocations are acquired that render the lymphoma antigen-independent. The recurrent translocation t(11;18)(q21;q21) is associated with failure to respond to antibiotic therapy and increased rate of dissemination. This translocation creates the API2-MALT1 fusion oncoprotein, which comprises the amino terminus of inhibitor of apoptosis 2 (API2 or cIAP2) fused to the carboxy terminus of MALT1. A common characteristic of chromosomal translocations in MALT lymphoma, including t(11;18), is that genes involved in the regulation of the NF-κB transcription factor are targeted by the translocations, and these genetic perturbations thereby result in deregulated, constitutive NF-κB stimulation. In the last decade, great insights into the roles of API2 and MALT1 in NF-κB signaling have been made. For example, recent pivotal studies have uncovered the long sought-after proteolytic activity of MALT1 and have demonstrated its critical involvement in the survival of certain lymphomas. Here, we review the current understanding of the role of MALT1 in normal lymphocyte function and lymphomagenesis. We then highlight our recent work that has revealed an intriguing link between the proteolytic activity of the API2-MALT1 fusion and its ability to influence lymphomagenesis by cleaving a key NF-κB regulatory protein, NF-κB-inducing kinase.

  10. Manipulating API and AOD data to distinguish transportation of aerosol at high altitude in Penang, Malaysia

    Science.gov (United States)

    Tan, F.; Lim, H. S.; Abdullah, K.; Yoon, T. L.; Matjafri, M. Z.; Holben, B.

    2014-02-01

    Air pollution index (API) is an index commonly used in Malaysia to determine the air quality level. It is a ground truth data measurement which is unable to unambiguously quantify air quality level at higher atmosphere. On the other hand, aerosol optical depth (AOD) from AERONET data obtained using sun photometer provides reading of the air quality for a column of atmosphere from ground surface. We first determine the quantitative correlation between the API and AOD data collected in Penang, Malaysia, between January - September, 2012, using two independent methods, one based on regression analysis and the other interpolation. Our purpose is to establish a systematic numerical procedure to determine whether aerosol transported in high altitude from other location has occurred. Two independent methods for establishing the quantitative relationship between the API and AOD data were used as a way to facilitate the verification of our approach. In our method, data from southwest monsoon period (August to September) were used as "calibration dataset" to establish the quantitative correlation between the AOD and API data. The established calibrated coefficients is then used to predict the AOD of other months, which are then compared against the data actually measured. Discrepancy between the predicted and measured AOD data can then be interpreted as an indication of whether the atmosphere at high altitude is polluted by aerosol transported from other location. If the predicted AOD is much larger than that measured, back trajectory analysis was applied to identify the aerosol transported source. This procedure is very helpful to investigate the aerosol transportation and distribution patterns during monsoon and non monsoon periods.

  11. The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera

    OpenAIRE

    Jones, Andrew K.; Raymond-Delpech, Valerie; Steeve H Thany; Gauthier, Monique; Sattelle, David B.

    2006-01-01

    Nicotinic acetylcholine receptors (nAChRs) mediate fast cholinergic synaptic transmission and play roles in many cognitive processes. They are under intense research as potential targets of drugs used to treat neurodegenerative diseases and neurological disorders such as Alzheimer's disease and schizophrenia. Invertebrate nAChRs are targets of anthelmintics as well as a major group of insecticides, the neonicotinoids. The honey bee, Apis mellifera, is one of the most beneficial insects worldw...

  12. Hydrocarbon Composition of Beeswax (Apis Mellifera) Collected from Light and Dark Coloured Combs

    OpenAIRE

    Waś Ewa; Szczęsna Teresa; Rybak-Chmielewska Helena

    2014-01-01

    The hydrocarbon composition of beeswax secreted by Apis mellifera was characterised. In the studies, analyses were made of virgin beeswax (obtained from light combs, socalled „wild-built combs“) that was collected at different dates, and beeswax obtained from dark combs („brood combs“). A qualitative analysis did not show any differences in the hydrocarbon composition of beeswax originating from light and dark coloured combs. The same hydrocarbons (n-alkanes, alkenes, and dienes) were identif...

  13. Microstructure and mechanical properties of two API steels for iron ore pipelines.

    OpenAIRE

    Godefroid,Leonardo Barbosa; Cândido,Luiz Cláudio; Toffolo,Rodrigo Vicente Bayão; Barbosa,Luiz Henrique Soares

    2014-01-01

    This research compares the mechanical behavior of two API steels (X60 and X70) used in the longest pipeline in the world for the conveyance of iron ore. Tensile tests, Charpy impact tests, CTOD tests and fatigue crack growth tests are performed at ambient temperature. Metallographic examination showed a banded microstructure consisting of polygonal ferrite and pearlite in both steels, with smaller grain size and the presence of a small quantity of bainite in the X70 steel. All the mechanical ...

  14. Galenics: studies of the toxicity and distribution of sugar substitutes on Apis mellifera

    OpenAIRE

    RADEMACHER, Eva; Fahlberg, Anja; Raddatz, Marlene; Schneider, Saskia; Voigt, Kathrin

    2013-01-01

    International audience; The aim of this study was to find a substitute to sugar water in medicinal treatments of honey bee colonies with the same properties but without being ingested by bees or being toxic to them. Tylose MH, sorbitol and glycerol were tested for their attractiveness to Apis mellifera, their application ability, toxicity via individual application and distribution in small groups respectively a small colony. Neither of the substances proved attractive or toxic. All had good ...

  15. Use of oxalic acid to control Varroa destructor in honeybee (Apis mellifera L.) colonies

    OpenAIRE

    Akyol, Ethem; YENİNAR, Halil

    2009-01-01

    This study was carried out to determine the effects of oxalic acid (OA) on reducing Varroa mite (Varroa destructor) populations in honeybee (Apis mellifera L.) colonies in the fall. Twenty honeybee colonies, in wooden Langstroth hives, were used in this experiment. Average Varroa infestation levels (%) of the OA and control groups were 25.87% and 24.57% on adult workers before the treatments. The OA treatments were applied twice, on 3 November and 13 November 2006. Average Varroa infestation ...

  16. Phosphorus 30 CH to control Varroa population in Apis mellifera colonies

    OpenAIRE

    Moscatelli, Francesca; Pietropaoli, Marco; Brocherel, Giuseppina; Martini, Andrea; Formato, Giovanni

    2016-01-01

    Varroa destructor is considered to be the main cause of European honeybee (Apis mellifera ssp. Linnaeus 1758) colony losses. The use of homeopathic products in veterinary practices has consistently increased in the last 50 years, but limited data are available on the application of homeopathic treatments to honeybees. The aims of this study were to investigate the acaricide efficacy and tolerability for honeybees treated for 35 days with the homeopathic product Phosphorus 30 CH. Methods...

  17. Transcriptional markers of sub-optimal nutrition in developing Apis mellifera nurse workers

    OpenAIRE

    Corby-Harris, Vanessa; Jones, Beryl M.; Walton, Alexander; Schwan, Melissa R; Kirk E Anderson

    2014-01-01

    Background Honey bees (Apis mellifera) contribute substantially to the worldwide economy and ecosystem health as pollinators. Pollen is essential to the bee’s diet, providing protein, lipids, and micronutrients. The dramatic shifts in physiology, anatomy, and behavior that accompany normal worker development are highly plastic and recent work demonstrates that development, particularly the transition from nurse to foraging roles, is greatly impacted by diet. However, the role that diet plays ...

  18. Studies of the eddy structure in the lower ionosphere by the API technique

    Science.gov (United States)

    Bakhmetieva, Nataliya V.; Grigoriev, Gennadii I.; Lapin, Victor G.

    2016-07-01

    We present a new application of the API technique to study of turbulent phenomena in the lower ionosphere. The main objective of these studies is experimental diagnostics of natural ordered eddy structures at the altitudes of the mesosphere and lower thermosphere, such as those that occur when internal gravity waves propagate in stratified flows in the atmospheric boundary layer. To this end, we considered the impact of eddy motions in the mesosphere and lower thermosphere on the relaxation time and the frequency of the signal scattered by periodic irregularities. Theoretical study of eddy structures base on experiments using SURA heating facility (56,14 N; 44,1 W). It is known, artificial periodic irregularities (APIs) are formed in the field of the powerful standing wave as a result of the interference of the incident wave and reflected from the ionosphere (Belikovich et al., Ionospheric Research by Means of Artificial Periodic Irregularities - 2002. Katlenburg-Lindau, Germany. Copernicus GmbH. 160 p.). The relaxation or decay of the periodic structure is specified by the ambipolar diffusion process. The atmospheric turbulence causes reduction of the amplitude and decay time of the API scattered signal in comparison with the diffusion time. We found a relation between the eddy period and the characteristic decay time of scattered signal, for which the synchronism of the waves scattered by a periodic structure is broken. Besides, it is shown, when the eddy structure moves by a horizontal wind exists at these heights, the frequency of the radio wave scattered by API structure will periodically increase and decrease compared with the frequency of the radiated diagnostic (probing) radio-wave. The work was supported by the Russian Science Foundation under grant No 14-12-00556.

  19. Comparison of ground based indices (API and AQI) with satellite based aerosol products.

    Science.gov (United States)

    Zheng, Sheng; Cao, Chun-Xiang; Singh, Ramesh P

    2014-08-01

    Air quality in mega cities is one of the major concerns due to serious health issues and its indirect impact to the climate. Among mega cities, Beijing city is considered as one of the densely populated cities with extremely poor air quality. The meteorological parameters (wind, surface temperature, air temperature and relative humidity) control the dynamics and dispersion of air pollution. China National Environmental Monitoring Centre (CNEMC) started air pollution index (API) as of 2000 to evaluate air quality, but over the years, it was felt that the air quality is not well represented by API. Recently, the Ministry of Environmental Protection (MEP) of the People's Republic of China (PRC) started using a new index "air quality index (AQI)" from January 2013. We have compared API and AQI with three different MODIS (MODIS - Moderate Resolution Imaging SpectroRadiometer, onboard the Terra/Aqua satellites) AOD (aerosol optical depth) products for ten months, January-October, 2013. The correlation between AQI and Aqua Deep Blue AOD was found to be reasonably good as compared with API, mainly due to inclusion of PM2.5 in the calculation of AQI. In addition, for every month, the correlation coefficient between AQI and Aqua Deep Blue AOD was found to be relatively higher in the month of February to May. According to the monthly average distribution of precipitation, temperature, and PM10, the air quality in the months of June-September was better as compared to those in the months of February-May. AQI and Aqua Deep Blue AOD show highly polluted days associated with dust event, representing true air quality of Beijing.

  20. An analysis of the intrinsic cross-correlations between API and meteorological elements using DPCCA

    Science.gov (United States)

    Shen, Chen-hua; Li, Cao-ling

    2016-03-01

    In order to reveal the intrinsic cross-correlations between air pollution index (API) records and synchronously meteorological elements data, the detrended partial cross-correlation (DPCC) coefficients are analyzed using a detrended partial cross-correlation analysis (DPCCA). DPCC coefficients for different spatial locations and seasons are calculated and compared. The results show that DPCCA can uncover intrinsic cross-correlations between API and meteorological elements, and most of their interactional mechanisms can be explained. DPCC coefficients are either positive or negative, and vary with spatial locations and seasons, with consistently interactional mechanisms. More remarkable, we find that detrended cross-correlation analysis can present the cross-correlations between the fluctuations in two nonstationary time series, but this cross-correlation does not always fully reflect the interactional mechanism for the original time series. Despite this, DPCCA is recommended as a comparatively reliable method for revealing intrinsic cross-correlations between API and meteorological elements, and it can also be useful for our understanding of their interactional mechanisms.