WorldWideScience

Sample records for acid utilization final

  1. Economic assessment of the utilization of lead-acid batteries in electric utility systems. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, A.C.; Hynds, J.A.; Nevius, D.R.; Nunan, G.A.; Sweetman, N.

    1977-04-01

    Specific applications in which lead--acid batteries might be economically competitive on an electric utility system are identified. Particular attention is given to searching the Public Service Electric and Gas Company (PSE and G) system for installations of batteries which could defer or cancel costly transmission and/or distribution projects. Although the transmission and distribution data are based on specific applications on the PSE and G system, the generation data are based on a national reference system. The report analyzes and summarizes all costs and savings attributable to lead--acid batteries. 40 figures, 78 tables. (RWR)

  2. Module Utilization Committee. Final report

    Energy Technology Data Exchange (ETDEWEB)

    None

    1984-03-01

    Photovoltaic collector modules were declared surplus to the needs of the US Department of Energy. The Module Utilization Committee was formed to make appropriate disposition of the surplus modules. The final report of that committee accounts for that disposition. The membership and activities of the committee are set forth and the results of its activities are reported.

  3. Utilization of used oil. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Mascetti, G.J.; White, H.M.

    1978-08-01

    This report assesses the potential impact of re-refining used automotive and industrial lubricating oils on the national petroleum consumption. The technical base for this assessment is derived from a comprehensive review of the processes utilized in re-refining used oil and those processes used to produce lube oil from crude. Both existing and recently proposed processes are considered. Additionally, an extensive review of processes described in the patent literature is provided. Re-refining processes are surveyed and evaluated. Process descriptions are provided; hardware is identified; and process energy and economic requirements are calculated. Factors affecting the profitability of a re-refining operation are discussed. Economic projections of the demand for lube oil and the ability to satisfy this demand from crude oil are made and the value of lube oil as a vital resource and the need for conservation are addressed. Other factors related to re-refining are discussed, including lube oil characteristics, degradation, lube oil quality and engine sequence testing, and legislative and institutional barriers. Finally, an energy assessment of used oil utilization is made. Two options are considered in this assessment: (1) all used oil is re-refined and recycled back to lube oil; (2) all used oil is burned to recover its heat content.

  4. Detection and Assessment Using Positron Emission Tomography of Genetically Determined Defects in Myocardial Fatty Acid Utilization. Final report, 8/1/93-6/30/97

    Energy Technology Data Exchange (ETDEWEB)

    Bergmann, Steven R.

    2000-04-09

    An approach using positron emission tomography (PET) was developed, validated and used to measure myocardial fatty acid metabolism in patients with inherited forms of heart failure. Abnormalities were correlated with the severity of the clinical illness. The approach developed was also shown to identify abnormalities in myocardial fatty acid metabolism in some patients with acquired forms of heart failure. The PET technique thus permits identification of abnormal fatty acid metabolism and provides an approach to evaluate the efficacy of interventional strategies.

  5. Utility guidelines for reactor noise analysis: Final report

    Energy Technology Data Exchange (ETDEWEB)

    Sweeney, F.J.

    1987-02-01

    Noise analysis techniques have been extensively utilized to monitor the health and performance of nuclear power plant systems. However, few utilities have adequate programs to effectively utilize these techniques. These programs usually provide low-quality data, which can lead to misinterpretation and false alarms. The objective of this work is to provide utilities and noise analysts with guidelines for data acquisition, data analysis, and interpretation of noise analysis results for surveillance and diagnosis of reactor systems.

  6. Acid rain information book. Draft final report

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-12-01

    Acid rain is one of the most widely publicized environmental issues of the day. The potential consequences of increasingly widespread acid rain demand that this phenomenon be carefully evaluated. Reveiw of the literature shows a rapidly growing body of knowledge, but also reveals major gaps in understanding that need to be narrowed. This document discusses major aspects of the acid rain phenomenon, points out areas of uncertainty, and summarizes current and projected research by responsible government agencies and other concerned organizations.

  7. Least-cost utility planning consumer participation manual. [Final report

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, C.; Wellinghoff, J.; Goldberg, F.

    1989-12-31

    This manual is designed to provide guidance to state consumer advocates and other state consumer groups interested in either initiating and/or participating in an Least-Cost Utility Planning (LCUP) process in their state. Least cost utility planning examined primarily as a regulatory framework to be implemented by an appropriate state authority -- usually the public utility commission -- for the benefit of the state`s citizens and electric utility customers. LCUP is also a planning process to be used by investor owned and public utilities to select, support and justify future expenditures in resource additions. This manual is designed as a ``How-To`` manual for implementing and participating in a statewide LCUP process. Its goal is to guide the reader through the LCUP maze so that meaningful, forward-looking, and cost minimizing electric utility planning can be initiated and sustained in your state.

  8. Organic acid mediated repression of sugar utilization in rhizobia.

    Science.gov (United States)

    Iyer, Bhagya; Rajput, Mahendrapal Singh; Jog, Rahul; Joshi, Ekta; Bharwad, Krishna; Rajkumar, Shalini

    2016-11-01

    Rhizobia are a class of symbiotic diazotrophic bacteria which utilize C4 acids in preference to sugars and the sugar utilization is repressed as long as C4 acids are present. This can be manifested as a diauxie when rhizobia are grown in the presence of a sugar and a C4 acid together. Succinate, a C4 acid is known to repress utilization of sugars, sugar alcohols, hydrocarbons, etc by a mechanism termed as Succinate Mediated Catabolite Repression (SMCR). Mechanism of catabolite repression determines the hierarchy of carbon source utilization in bacteria. Though the mechanism of catabolite repression has been well studied in model organisms like E. coli, B. subtilis and Pseudomonas sp., mechanism of SMCR in rhizobia has not been well elucidated. C4 acid uptake is important for effective symbioses while mutation in the sugar transport and utilization genes does not affect symbioses. Deletion of hpr and sma0113 resulted in the partial relief of SMCR of utilization of galactosides like lactose, raffinose and maltose in the presence of succinate. However, no such regulators governing SMCR of glucoside utilization have been identified till date. Though rhizobia can utilize multitude of sugars, high affinity transporters for many sugars are yet to be identified. Identifying high affinity sugar transporters and studying the mechanism of catabolite repression in rhizobia is important to understand the level of regulation of SMCR and the key regulators involved in SMCR.

  9. Siting guidelines for utility application of wind turbines. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Pennell, W.T.

    1983-01-01

    Utility-oriented guidelines are described for identifying viable sites for wind turbines. Topics and procedures are also discussed that are important in carrying out a wind turbine siting program. These topics include: a description of the Department of Energy wind resource atlases; procedures for predicting wind turbine performance at potential sites; methods for analyzing wind turbine economics; procedures for estimating installation and maintenance costs; methods for anlayzing the distribution of wind resources over an area; and instrumentation for documenting wind behavior at potential sites. The procedure described is applicable to small and large utilities. Although the procedure was developed as a site-selection tool, it can also be used by a utility who wishes to estimate the potential for wind turbine penetration into its future generation mix.

  10. Siting guidelines for utility application of wind turbines. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Pennell, W.T.

    1983-01-01

    Utility-oriented guidelines are described for identifying viable sites for wind turbines. Topics and procedures are also discussed that are important in carrying out a wind turbine siting program. These topics include: a description of the Department of Energy wind resource atlases; procedures for predicting wind turbine performance at potential sites; methods for analyzing wind turbine economics; procedures for estimating installation and maintenance costs; methods for anlayzing the distribution of wind resources over an area; and instrumentation for documenting wind behavior at potential sites. The procedure described is applicable to small and large utilities. Although the procedure was developed as a site-selection tool, it can also be used by a utility who wishes to estimate the potential for wind turbine penetration into its future generation mix.

  11. Geothermal resource utilization: paper and cane sugar industries. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Hornburg, C.D.; Morin, O.J.

    1975-03-01

    This study was made as a specific contribution to an overall report by the United States in the area of industrial utilization of geothermal resources. This is part of an overall study in non-electrical uses of geothermal resources for a sub-committee of the North Atlantic Treaty Organization. This study was restricted to the geopressured zone along the Northern Gulf of Mexico Coast. Also, it was limited to utilizing the thermal energy of this ''geoenergy'' resource for process use in the Pulp and Paper Industry and Cane Sugar Industry. For the selected industries and resource area, this report sets forth energy requirements; identifies specific plant and sites; includes diagrams of main processes used; describes process and equipment modifications required; describes energy recovery systems; sets forth waste disposal schemes and problems; and establishes the economics involved. The scope of work included considerable data collection, analysis and documentation. Detailed technical work was done concerning existing processes and modifications to effectively utilize geothermal energy. A brief survey was made of other industries to determine which of these has a high potential for utilizing geothermal energy.

  12. Dechlorination Technology Manual. Final report. [Utility cooling water discharge systems

    Energy Technology Data Exchange (ETDEWEB)

    Aschoff, A.F.; Chiesa, R.J.; Jacobs, M.H.; Lee, Y.H.; Mehta, S.C.; Meko, A.C.; Musil, R.R.; Sopocy, D.M.; Wilson, J.A.

    1984-11-01

    On November 19, 1982, the United States Environmental Protection Agency (EPA) promulgated regulations severely restricting chlorination practices as they relate to utility cooling water discharge systems. EPRI authorized the preparation of a manual on dechlorination technology to assist utilities in evaluating the various alternatives available to them to meet these new requirements. The Dechlorination Technology Manual emphasizes the engineering aspects involved in the selection and design of dechlorination systems. However, background information is included concerning chemistry, regulatory requirements, environmental considerations and aquatic impacts. There is also a brief discussion of the various alternatives to dechlorination. Case studies are given to acquaint the user with the use of the manual for the design of chlorination facilities given various site-related characteristics, such as salt versus fresh waters. Numerous graphs and tables are presented to facilitate the selection and design process. 207 references, 66 figures, 60 tables.

  13. A knowledge based model of electric utility operations. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-08-11

    This report consists of an appendix to provide a documentation and help capability for an analyst using the developed expert system of electric utility operations running in CLIPS. This capability is provided through a separate package running under the WINDOWS Operating System and keyed to provide displays of text, graphics and mixed text and graphics that explain and elaborate on the specific decisions being made within the knowledge based expert system.

  14. Hawaii alternative fuels utilization program. Phase 3, final report

    Energy Technology Data Exchange (ETDEWEB)

    Kinoshita, C.M.; Staackmann, M.

    1996-08-01

    The Hawaii Alternative Fuels Utilization Program originated as a five-year grant awarded by the US Department of Energy (USDOE) to the Hawaii Natural Energy Institute (HNEI) of the University of Hawaii at Manoa. The overall program included research and demonstration efforts aimed at encouraging and sustaining the use of alternative (i.e., substitutes for gasoline and diesel) ground transportation fuels in Hawaii. Originally, research aimed at overcoming technical impediments to the widespread adoption of alternative fuels was an important facet of this program. Demonstration activities centered on the use of methanol-based fuels in alternative fuel vehicles (AFVs). In the present phase, operations were expanded to include flexible fuel vehicles (FFVs) which can operate on M85 or regular unleaded gasoline or any combination of these two fuels. Additional demonstration work was accomplished in attempting to involve other elements of Hawaii in the promotion and use of alcohol fuels for ground transportation in Hawaii.

  15. Solar thermal repowering utility value analysis. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, R.; Day, J.; Reed, B.; Malone, M.

    1979-12-01

    The retrofit of solar central receiver energy supply systems to existing steam-electric generating stations (repowering) is being considered as a major programmatic thrust by DOE. The determination of a government response appropriate to the opportunities of repowering is an important policy question, and is the major reason for the analysis. The study objective is to define a government role in repowering that constitutes an efficient program investment in pursuit of viable private markets for heliostat-based energy systems. In support of that objective, the study is designed to identify the scope and nature of the repowering opportunity within the larger context of its contributions to central receiver technology development and commercialization. The Supply and Integration Tasks are documented elsewhere. This report documents the Demand Task, determining and quantifying the sources of the value of repowering and of central receiver technology in general to electric utilities. The modeling tools and assumptions used in the Demand Task are described and the results are presented and interpreted. (MCW)

  16. Climate Change Vulnerability Assessments: Four Case Studies of Water Utility Practices (2011 Final)

    Science.gov (United States)

    EPA has released the final report titled, Climate Change Vulnerability Assessments: Four Case Studies of Water Utility Practices. This report was prepared by the National Center for Environmental Assessment's Global Climate Research Staff in the Office of Research and D...

  17. Climate Change Vulnerability Assessments: Four Case Studies of Water Utility Practices (2011 Final)

    Science.gov (United States)

    EPA has released the final report titled, Climate Change Vulnerability Assessments: Four Case Studies of Water Utility Practices. This report was prepared by the National Center for Environmental Assessment's Global Climate Research Staff in the Office of Research and D...

  18. The utility`s role in the future of PC services and the NII. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-01-01

    The paper is a report by C3 Communications (formerly CSW Communications) summarizing the experience and lessons learned during an 18 month Department of Energy Grant to evaluate how electric utilities can further the growth of the NII by developing a hybrid Internet-energy management service. In addition, the project was also expanded to include evaluation of residential gateway issues, in particular the energy management aspects of this developing area. The report is broken up into three sections, the first section focuses on the issues surrounding the start-up of a traditional ISP and provides a road map for utilities interested in this area. The second section provides an overview of the Internet energy management projects which were undertaken and the key lessons learned from each. Lastly, the third section outlines the gateway progress made during the grant and provides some of the recommendations which the work produced.

  19. Novel technology for sewage sludge utilization: preparation of amino acids chelated trace elements (AACTE) fertilizer.

    Science.gov (United States)

    Liu, Yangsheng; Kong, Sifang; Li, Yaqiong; Zeng, Hui

    2009-11-15

    This study developed a novel technology for sewage sludge utilization. The bacteria proteins in the sewage sludge were extracted to produce the amino acid chelated trace elements (AACTE) fertilizer by virtue of several chemical processes. Firstly, the sewage sludge was hydrolyzed under hot hydrochloric acid solution to obtain protein solution. The effects of hydrolysis temperature, reaction time and pH on the extraction ratio of protein from the sewage sludge were investigated. Secondly, the protein solution was further hydrolyzed into amino acids under hot acid condition. The effects of the HCl dosage, hydrolysis temperature and reaction time on the yields of amino acids were investigated in detail. Thirdly, the raw amino acids solution was purified by activated carbon decolorization and glacial acetic acid dissolution. Finally, the purified amino acids were used to produce the AACTE fertilizer by chelating with trace elements. Results showed that, under optimum hydrolysis conditions, 78.5% of protein was extracted from the sewage sludge and the amino acids yield was 10-13 g per 100g of dry sludge. The AACTE fertilizer produced was in accordance with China Standard for Amino Acids Foliar Fertilizer. This novel technology is more environmentally friendly compared with the conventional sludge treatments.

  20. The retinal pigment epithelium utilizes fatty acids for ketogenesis.

    Science.gov (United States)

    Adijanto, Jeffrey; Du, Jianhai; Moffat, Cynthia; Seifert, Erin L; Hurle, James B; Philp, Nancy J

    2014-07-25

    Every day, shortly after light onset, photoreceptor cells shed approximately a tenth of their outer segment. The adjacent retinal pigment epithelial (RPE) cells phagocytize and digest shed photoreceptor outer segment, which provides a rich source of fatty acids that could be utilized as an energy substrate. From a microarray analysis, we found that RPE cells express particularly high levels of the mitochondrial HMG-CoA synthase 2 (Hmgcs2) compared with all other tissues (except the liver and colon), leading to the hypothesis that RPE cells, like hepatocytes, can produce β-hydroxybutyrate (β-HB) from fatty acids. Using primary human fetal RPE (hfRPE) cells cultured on Transwell filters with separate apical and basal chambers, we demonstrate that hfRPE cells can metabolize palmitate, a saturated fatty acid that constitutes .15% of all lipids in the photoreceptor outer segment, to produce β-HB. Importantly, we found that hfRPE cells preferentially release β-HB into the apical chamber and that this process is mediated primarily by monocarboxylate transporter isoform 1 (MCT1). Using a GC-MS analysis of (13)C-labeled metabolites, we showed that retinal cells can take up and metabolize (13)C-labeled β-HB into various TCA cycle intermediates and amino acids. Collectively, our data support a novel mechanism of RPE-retina metabolic coupling in which RPE cells metabolize fatty acids to produce β-HB, which is transported to the retina for use as a metabolic substrate.

  1. Sex Steroid Modulation of Fatty Acid Utilization and Fatty Acid Binding Protein Concentration in Rat Liver

    Science.gov (United States)

    Ockner, Robert K.; Lysenko, Nina; Manning, Joan A.; Monroe, Scott E.; Burnett, David A.

    1980-01-01

    The mechanism by which sex steroids influence very low density hepatic lipoprotein triglyceride production has not been fully elucidated. In previous studies we showed that [14C]oleate utilization and incorporation into triglycerides were greater in hepatocyte suspensions from adult female rats than from males. The sex differences were not related to activities of the enzymes of triglyceride biosynthesis, whereas fatty acid binding protein (FABP) concentration in liver cytosol was greater in females. These findings suggested that sex differences in lipoprotein could reflect a sex steroid influence on the availability of fatty acids for hepatocellular triglyceride biosynthesis. In the present studies, sex steroid effects on hepatocyte [14C]oleate utilization and FABP concentration were investigated directly. Hepatocytes from immature (30-d-old) rats exhibited no sex differences in [14C]oleate utilization. With maturation, total [14C]oleate utilization and triglyceride biosynthesis increased moderately in female cells and decreased markedly in male cells; the profound sex differences in adults were maximal by age 60 d. Fatty acid oxidation was little affected. Rats were castrated at age 30 d, and received estradiol, testosterone, or no hormone until age 60 d, when hepatocyte [14C]oleate utilization was studied. Castration virtually eliminated maturational changes and blunted the sex differences in adults. Estradiol or testosterone largely reproduced the appropriate adult pattern of [14C]oleate utilization regardless of the genotypic sex of the treated animal. In immature females and males, total cytosolic FABP concentrations were similar. In 60-d-old animals, there was a striking correlation among all groups (females, males, castrates, and hormone-treated) between mean cytosolic FABP concentration on the one hand, and mean total [14C]oleate utilization (r = 0.91) and incorporation into triglycerides (r = 0.94) on the other. In 30-d-old animals rates of [14C

  2. The Michigan regulatory incentives study for electric utilities. Phase 1, Final report

    Energy Technology Data Exchange (ETDEWEB)

    Reid, M.W.; Weaver, E.M. [Barakat and Chamberlin, Inc., Oakland, CA (United States)

    1991-06-17

    This is the final report of Phase I of the Michigan Regulatory Incentives Study for Electric Utilities, a three-phase review of Michigan`s regulatory system and its effects on resource selection by electric utilities. The goal of Phase I is to identify and analyze financial incentive mechanisms that encourage selection of resources in accord with the principles of integrated resource planning (IRP) or least-cost planning (LCP). Subsequent study phases will involve further analysis of options and possibly a collaborative formal effort to propose regulatory changes. The Phase I analysis proceeded in three steps: (1) identification and review of existing regulatory practices that affect utilities; selection of resources, particularly DSM; (2) preliminary analysis of ten financial mechanisms, and selection of three for further study; (3) detailed analysis of the three mechanisms, including consideration of how they could be implemented in Michigan and financial modeling of their likely impacts on utilities and ratepayers.

  3. Fatty acid synthesis is inhibited by inefficient utilization of unusual fatty acids for glycerolipid assembly.

    Science.gov (United States)

    Bates, Philip D; Johnson, Sean R; Cao, Xia; Li, Jia; Nam, Jeong-Won; Jaworski, Jan G; Ohlrogge, John B; Browse, John

    2014-01-21

    Degradation of unusual fatty acids through β-oxidation within transgenic plants has long been hypothesized as a major factor limiting the production of industrially useful unusual fatty acids in seed oils. Arabidopsis seeds expressing the castor fatty acid hydroxylase accumulate hydroxylated fatty acids up to 17% of total fatty acids in seed triacylglycerols; however, total seed oil is also reduced up to 50%. Investigations into the cause of the reduced oil phenotype through in vivo [(14)C]acetate and [(3)H]2O metabolic labeling of developing seeds surprisingly revealed that the rate of de novo fatty acid synthesis within the transgenic seeds was approximately half that of control seeds. RNAseq analysis indicated no changes in expression of fatty acid synthesis genes in hydroxylase-expressing plants. However, differential [(14)C]acetate and [(14)C]malonate metabolic labeling of hydroxylase-expressing seeds indicated the in vivo acetyl-CoA carboxylase activity was reduced to approximately half that of control seeds. Therefore, the reduction of oil content in the transgenic seeds is consistent with reduced de novo fatty acid synthesis in the plastid rather than fatty acid degradation. Intriguingly, the coexpression of triacylglycerol synthesis isozymes from castor along with the fatty acid hydroxylase alleviated the reduced acetyl-CoA carboxylase activity, restored the rate of fatty acid synthesis, and the accumulation of seed oil was substantially recovered. Together these results suggest a previously unidentified mechanism that detects inefficient utilization of unusual fatty acids within the endoplasmic reticulum and activates an endogenous pathway for posttranslational reduction of fatty acid synthesis within the plastid.

  4. Phytic acid and phytase: implications for protein utilization by poultry.

    Science.gov (United States)

    Cowieson, A J; Acamovic, T; Bedford, M R

    2006-05-01

    The effect of the ingestion of myo-inositol hexaphosphate (IP6) and phytase (EC 3.1.3.26) on the digestibility of casein was investigated using growing broiler chickens. A total of 64 female Ross broilers were used in a precision feeding study. One group of 8 birds was fed a solution of glucose to estimate endogenous losses. Seven groups, each of 8 birds, were fed either casein, casein + 1,000 units of phytase activity (FTU), casein + 2,000 FTU, casein + 0.5 g of IP6, casein + 0.5 g of IP6 + 1,000 FTU, casein + 1 g of IP6, or casein + 1 g of IP6 + 1,000 FTU. The excretion of DM, amino acids, nitrogen, minerals, and phytate-phosphorus was determined over a 48-h period and nutrient digestibility coefficients were calculated. Casein was found to be highly digestible, with true coefficients of DM, N, and amino acid digestibility of between 0.85 and 1.0. However, the ingestion of IP6 reduced (P IP6 with phytase improved (P IP6 with no supplemental phytase. The excretion of endogenous minerals was increased (P IP6 and reduced (P IP6 with phytase. In the absence of exogenous phytase, the recovery of phytate-P in excreta was approximately 80%. However, the recovery of phytate-P was significantly reduced by the addition of exogenous phytase to the IP6/casein mixture. It can be concluded that the ingestion of IP6 reduces the digestibility coefficients of amino acids and the metabolizability of nitrogen of casein. This is likely to be mediated partially through increased endogenous losses. However, the addition of phytase can partially ameliorate the detrimental effects of IP6 on protein utilization.

  5. Commercialization of PV-powered pumping systems for use in utility PV service programs. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The project described in this report was a commercialization effort focused on cost-effective remote water pumping systems for use in utility-based photovoltaic (PV) service programs. The project combined a commercialization strategy tailored specifically for electric utilities with the development of a PV-powered pumping system that operates conventional ac pumps rather than relying on the more expensive and less reliable PV pumps on the market. By combining these two attributes, a project goal was established of creating sustained utility purchases of 250 PV-powered water pumping systems per year. The results of each of these tasks are presented in two parts contained in this Final Summary Report. The first part summarizes the results of the Photovoltaic Services Network (PSN) as a new business venture, while the second part summarizes the results of the Golden Photon system installations. Specifically, results and photographs from each of the system installations are presented in this latter part.

  6. Management support services to the Office of Utility Technologies. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    1993-12-16

    The Office of Utility Technologies works cooperatively with industry and the utility sector to realize the market potential for energy efficiency and renewable energy technologies. Under this contract, BNF has provided management support services for OUT R&D activities for the following Program offices: (1) Office of Energy Management; (2) Office of Solar Energy Conversion; (3) Office of Renewable Energy Conversion; and (4) Deputy Assistant Secretary. During the period between 4/17/91 and 9/17/93, BNF furnished the necessary personnel, equipment, materials, facilities and travel required to provide management support services for each of the above Program Offices. From 9/18/93 to 12/17/93, BNF has been involved in closeout activities, including final product deliverables. Research efforts that have been supported in these Program Offices are: (1) for Energy Management -- Advanced Utility Concepts Division; Utility Systems Division; Integrated Planning; (2) for Solar Energy Conversion -- Photovoltaics Division; Solar Thermal and Biomass Power Division; (3) for Renewable Energy Conversion -- Geothermal Division; Wind, Hydroelectric and Ocean Systems Division; (4) for the Deputy Assistant Secretary -- support as required by the Supporting Staff. This final report contains summaries of the work accomplished for each of the Program Offices listed above.

  7. Utilization of milk amino acids by the suckling Iberian piglet.

    Science.gov (United States)

    Aguinaga, M A; Gómez-Carballar, F; Nieto, R; Aguilera, J F

    2011-12-01

    Sixteen pure-bred Iberian (IB) sows were used in two trials to determine the efficiency of utilization of milk protein and amino acid (AA) for growth in suckling piglets. It was hypothesized that there may be one or more strongly limiting essential AA (EAA) responsible for the slow rate of growth of the IB piglet. This AA will show the highest fractional retention. Daily milk yield and composition were determined weekly over a 34-day lactation period. Within each litter, one piglet at birth and four piglets on d 35 of life were slaughtered. The protein content of the IB sow milk was similar to that reported for conventional breeds. However, branched-chain AA, Thr, Pro, Asp and Ala were in concentrations somewhat below the range of literature values and Arg and Met, substantially above it. Milk intake per piglet tended to be greater in Trial 2 (832 vs. 893 g/day respectively; p = 0.066). However, the IB piglets grew at 168 ± 3.3 g/day, irrespective of the trial. The whole-body protein of piglets at weaning and the protein deposited in their body during the lactating period showed very close AA pattern. Among EAA, His and Arg show the highest fractional retentions (g AA retained/g AA ingested) in whole-body tissues (1.019 ± 0.025 and 0.913 ± 0.017 respectively) and also the highest body to milk ratios (1.50 and 1.41 respectively). Gly and Ala presented, among non essential AA, the highest efficiencies of utilization for tissue deposition (1.803 ± 0.057 and 1.375 ± 0.026 respectively) and body to milk ratios (2.75 and 2.12 respectively). These results suggest that the low efficiency of utilization of milk protein and the low rate of gain of the IB suckling piglet can be explained by a marked shortage in His supply, in addition to the suboptimal milk provision of Arg, Gly and Ala.

  8. Acid rain compliance and coordination of state and federal utility regulation

    Energy Technology Data Exchange (ETDEWEB)

    Nordhaus, R.R. [Van Ness, Feldman, and Curtis, P.C., Washington, DC (United States)

    1993-07-01

    The Clean Air Act Amendments of 1990 (CAAA) impose new controls on emissions by electric utilities of the two major precursors of acid rain: sulfur dioxide and oxides of nitrogen. Utilities, and the utility holding company systems and power pools of which they are members, will be subject to extensive and costly compliance obligations under the new stature. Most of these utilities, utility systems, and power pools are regulated by more than one utility regulatory authority. Utility regulators will need to coordinate their policies for ratemaking and for review of acid rain compliance strategies if least-cost solutions are to be implemented without imposing on rate payers and utility shareholders the costs and risks of inconsistent regulatory determinations. This article outlines the scope of the coordination problem and spells out possible approaches that utility regulators may take in dealing with it. Topics covered include the following: the 1990 Clean Air Act Amendments; acid rain (SO2); acid rain (NOx); costs of compliance; implications for utility regulation - federal and state utility regulatory framework; potential jurisdictional conflicts under existing state/federal utility regulatory scheme - single utility, holding companies, power pools; Utility regulatory issues under the 1990 amendments - planning conflicts, operational conflicts; methods for dealing with potential jurisdictional conflicts; coordination mechanisms - informal consultation, rulemaking,coordination of adjudicatory proceedings, FERC rate filings.

  9. Uranium Leaching from Contaminated Soil Utilizing Rhamnolipid, EDTA, and Citric Acid

    Directory of Open Access Journals (Sweden)

    Sara Asselin

    2014-01-01

    Full Text Available Biosurfactants have recently gained attention as “green” agents that can be used to enhance the remediation of heavy metals and some organic matter in contaminated soils. The overall objective of this paper was to investigate rhamnolipid, a microbial produced biosurfactant, and its ability to leach uranium present in contaminated soil from an abandoned mine site. Soil samples were collected from two locations in northern Arizona: Cameron (site of open pit mining and Leupp (control—no mining. The approach taken was to first determine the total uranium content in each soil using a hydrofluoric acid digestion, then comparing the amount of metal removed by rhamnolipid to other chelating agents EDTA and citric acid, and finally determining the amount of soluble metal in the soil matrix using a sequential extraction. Results suggested a complex system for metal removal from soil utilizing rhamnolipid. It was determined that rhamnolipid at a concentration of 150 μM was as effective as EDTA but not as effective as citric acid for the removal of soluble uranium. However, the rhamnolipid was only slightly better at removing uranium from the mining soil compared to a purified water control. Overall, this study demonstrated that rhamnolipid ability to remove uranium from contaminated soil is comparable to EDTA and to a lesser extent citric acid, but, for the soils investigated, it is not significantly better than a simple water wash.

  10. Production development and utilization of Zimmer Station wet FGD by-products. Final report. Volume 4, A laboratory study conducted in fulfillment of Phase 2, Objective 1 titled. Inhibition of acid production in coal refuse amended with calcium sulfite and calcium sulfate - containing FGD solids

    Energy Technology Data Exchange (ETDEWEB)

    Hao, Y. L. [Ohio State Univ., Wooster, OH (United States); Dick, W. A. [Ohio State Univ., Wooster, OH (United States); Stehouwer, R. C. [Ohio State Univ., Wooster, OH (United States); Bigham, J. M. [Ohio State Univ., Wooster, OH (United States)

    1998-06-30

    combination effect was partially due to the positive interaction of CaS03 with CaC03 and fly ash on inhibition of acid leaching. In Chapter 3, CaS03-containing FGD was found to inhibit acid leaching from both fresh and aged coal refuse in large scale columns under simulated field conditions. During 39 weeks of leaching, the reduction of leachate acidity and Fe concentration and the increase ofleachate pH were significant (p <0.05) for the 22% FGD treatment with a linear response to increasing FGD rates (0%, 5.5%, 11%, and 22%). I conclude that CaS03 and CaS03-containing FGD have the ability to inhibit acid production in coal refuse and the inhibitory effect shown in this experiment is likely to occur under field conditions. Thus, the research results present a potential new method for mitigation of acid production in coal refuse and another beneficial utilization of FGD by-products.

  11. Metagenomic analysis of the rhizosphere soil microbiome with respect to phytic acid utilization.

    Science.gov (United States)

    Unno, Yusuke; Shinano, Takuro

    2013-01-01

    While phytic acid is a major form of organic phosphate in many soils, plant utilization of phytic acid is normally limited; however, culture trials of Lotus japonicus using experimental field soil that had been managed without phosphate fertilizer for over 90 years showed significant usage of phytic acid applied to soil for growth and flowering and differences in the degree of growth, even in the same culture pot. To understand the key metabolic processes involved in soil phytic acid utilization, we analyzed rhizosphere soil microbial communities using molecular ecological approaches. Although molecular fingerprint analysis revealed changes in the rhizosphere soil microbial communities from bulk soil microbial community, no clear relationship between the microbiome composition and flowering status that might be related to phytic acid utilization of L. japonicus could be determined. However, metagenomic analysis revealed changes in the relative abundance of the classes Bacteroidetes, Betaproteobacteria, Chlorobi, Dehalococcoidetes and Methanobacteria, which include strains that potentially promote plant growth and phytic acid utilization, and some gene clusters relating to phytic acid utilization, such as alkaline phosphatase and citrate synthase, with the phytic acid utilization status of the plant. This study highlights phylogenetic and metabolic features of the microbial community of the L. japonicus rhizosphere and provides a basic understanding of how rhizosphere microbial communities affect the phytic acid status in soil.

  12. Non-electric utilization of geothermal energy in the San Luis Valley, Colorado. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Vorum, M.; Coury, G.E.; Goering, S.W.; Fritzler, E.A.

    1978-02-01

    Information on the geothermal resources of the San Luis Valley, Colorado, has been gathered and reviewed and a preliminary, quantitative assessment of the magnitude and quality of resources present was carried out. Complete process designs were developed for the processes of producing crystal sugar from beets and for malting barley for use in the brewing industry, in each case adapting the processes to use a 302/sup 0/F geothermal water supply as the main process energy source. A parametric design analysis was performed for a major pipeline to be used to ship geothermal water, and thus deliver its heat, out of the San Luis Valley to three major Colorado cities along the eastern threshold of the Rocky Mountains. Cost estimates for capital equipment and energy utilization are presented. The analyses of the two process applications indicate favorable economics for conversion and operation as geothermally-heated plants. A major geothermal water pipeline for this region is seriously limited on achievement of the economy of scale by the physical absence of significant demand for heat energy. Finally, the development and utilization of Colorado's San Luis Valley geothermal groundwaters hold the potential to contribute to the prudent and beneficial management of that area's natural water resources systems.

  13. Comparative Genomics of Regulation of Fatty Acid and Branched-chain Amino Acid Utilization in Proteobacteria

    Energy Technology Data Exchange (ETDEWEB)

    Kazakov, Alexey E.; Rodionov, Dmitry A.; Arkin, Adam Paul; Dubchak, Inna; Gelfand, Mikhail S.; Alm, Eric

    2008-10-31

    Bacteria can use branched-chain amino acids (ILV, i.e. isoleucine, leucine, valine) and fatty acids (FA) as sole carbon and energy sources convering ILV into acetyl-CoA, propanoyl-CoA and propionyl-CoA, respectively. In this work, we used the comparative genomic approach to identify candidate transcriptional factors and DNA motifs that control ILV and FA utilization pathways in proteobacteria. The metabolic regulons were characterized based on the identification and comparison of candidate transcription factor binding sites in groups of phylogenetically related genomes. The reconstructed ILV/FA regulatory network demonstrates considerable variability and involves six transcriptional factors from the MerR, TetR and GntR families binding to eleven distinct DNA motifs. The ILV degradation genes in gamma- and beta-proteobacteria are mainly regulated by anovel regulator from the MerR family (e.g., LiuR in Pseudomonas aeruginosa) (40 species), in addition, the TetR-type regulator LiuQ was identified in some beta-proteobacteria (8 species). Besides the core set of ILV utilization genes, the LiuR regulon in some lineages is expanded to include genes from other metabolic pathways, such as the glyoxylate shunt and glutamate synthase in the Shewanella species. The FA degradation genes are controlled by four regulators including FadR in gamma-proteobacteria (34 species), PsrA in gamma- and beta-proteobacteria (45 species), FadP in beta-proteobacteria (14 species), and LiuR orthologs in alpha-proteobacteria (22 species). The remarkable variability of the regulatory systems associated with the FA degradation pathway is discussed from the functional and evolutionary points of view.

  14. Metagenomic Analysis of the Rhizosphere Soil Microbiome with Respect to Phytic Acid Utilization

    OpenAIRE

    Unno, Yusuke; Shinano, Takuro

    2012-01-01

    While phytic acid is a major form of organic phosphate in many soils, plant utilization of phytic acid is normally limited; however, culture trials of Lotus japonicus using experimental field soil that had been managed without phosphate fertilizer for over 90 years showed significant usage of phytic acid applied to soil for growth and flowering and differences in the degree of growth, even in the same culture pot. To understand the key metabolic processes involved in soil phytic acid utilizat...

  15. Utilization of milk fatty acids by the suckling Iberian piglets.

    Science.gov (United States)

    Aguinaga, M A; Haro, A; Lara, L; Gómez-Carballar, F; Nieto, R; Aguilera, J F

    2016-11-01

    A total of 16 pure-bred Iberian (IB) sows, all of them suckling six piglets, were used, eight of them in each of the two consecutive trials (1 and 2). Daily milk yield and composition were determined weekly over a 34-day lactation period. Within each litter, one piglet at birth and four piglets on day 35 of life were slaughtered. Milk intake per piglet tended to be greater in trial 2 (832 v. 893 g/day; P=0.066), but piglets grew at 168±3.3 g/day, irrespective of the trial. In the IB sow milk, the linoleic (LA) : linolenic (LNA) acid ratio averaged 14.6 and 15.2 in trial 1 and trial 2, respectively. A fivefold increase in piglet body fat content was observed over lactation (Pacids were very close to those in the milk consumed, suggesting direct deposition. Daily deposition of LA derivatives and of LNA and its derivatives was found to be extremely low (acid (ARA) in tissues of the IB piglet at birth disappeared throughout the lactating period. An overall fractional deposition for total fatty acids (FA) was 0.409. Fractional oxidation (disappearance) rates were 0.939 and 0.926 for n-6 and n-3 polyunsaturated FA. The overall rate of disappearance for the major non-essential FA (myristic, palmitic, palmitoleic, stearic and oleic acids), estimated as 1-the overall fractional deposition rate, was 0.546. It is concluded that the high degree of FA unsaturation, high oxidation rate of LA and LNA, and poor synthesis of ARA from LA and of docosahexaenoic acid from LNA found in the suckling piglet might increase the energy cost of whole-body fat accretion, a contributor to the observed low efficiency of use of milk energy for growth.

  16. 78 FR 1221 - Notice of Issuance of Final Determination Concerning Ponstel® (Mefenamic Acid) Capsules

    Science.gov (United States)

    2013-01-08

    ... (Mefenamic Acid) Capsules AGENCY: U.S. Customs and Border Protection, Department of Homeland Security. ACTION... acid) capsules. Based upon the facts presented, CBP has concluded in the final determination that India is the country of origin of the Ponstel (mefenamic acid) capsules for purposes of U.S. Government...

  17. 78 FR 64914 - Citric Acid and Certain Citrate Salts From Canada: Final Results of Antidumping Duty...

    Science.gov (United States)

    2013-10-30

    ... China: Antidumping Duty Orders, 74 FR 25703 (May 29, 2009) (Citric Acid Duty Orders). Period of Review...-others rate made effective by the LTFV investigation. See Citric Acid Duty Orders, 74 FR 25703. These... International Trade Administration Citric Acid and Certain Citrate Salts From Canada: Final Results...

  18. Acid precipitation: effects on forest and fish. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Overrein, L.N.; Seip, H.M.; Tollan, A.

    1980-12-01

    This interdisciplinary research program was launched in 1972 in response to concern in Scandinavian countries that acid precipitation was causing changes to the natural environment. A major hypothesis was that anthropogenic release of sulfur oxides and other pollutants may alter geobiochemical and biochemical cycles with consequences for the biota. The main research efforts were directed towards possible threats to forest and freshwater fish. Results of the entire program are summarized. Information is presented under the following section headings: emissions and transport; atmospheric deposits in Norway; water acidification - status and trends; chemical modifications of precipitation in contact with soil and vegetation; snow and snowmelt; land-use changes and acidification; effects of acid precipitation on soil productivity and plant growth; and effects of acid water on aquatic life.

  19. Utilization of ash from municipal solid waste combustion. Final report, Phase I

    Energy Technology Data Exchange (ETDEWEB)

    Jones, C.M.; Hartman, R.M.; Kort, D.; Rapues, N.

    1994-09-01

    This ash study investigates several aspects of Municipal Waste Combustion (MWC) ash utilization to develop an alternative to the present disposal practice of landfilling in a lined monofill. Ash was investigated as a daily or final cover for municipal waste in the landfill to prevent erosion and as a road construction aggregate. Samples of eight mixtures of ash and other materials, and one sample of soil were analyzed for chemical constituents. Biological tests on these mixters were conducted, along with erosion tests and sieve analyses. A chemical analysis of each sieve size was conducted. Geotechnical properties of the most promising materials were made. Findings to this point include: all ash samples take have passed the EPA TCLP testing; chemical analysis of bottom and combined ash samples indicate less than expected variability; selected ash mixtures exhibited very low coefficients of hydraulic conductivity; all but one of the ash mixtures exhibited greater erosion resistance than the currently used landfill cover material; MWC combined analysis indicates this is a viable alternative for landfill cover; MWC ash size reactions and chemical analysis show bottom and combined ash to be a viable alternative for road construction.

  20. Relationship between Uric Acid Level and Achievement Motivation. Final Report.

    Science.gov (United States)

    Mueller, Ernst F.; French, John R. P., Jr.

    In an investigation of the relationship of uric acid (a metabolic end product) to achievement, this study hypothesized that a person's serum urate level (a factor often associated with gout) is positively related to achievement need as well as indicators of actual achievement. (Speed of promotion and number of yearly publications were chosen as…

  1. Organic acid modeling and model validation: Workshop summary. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, T.J.; Eilers, J.M.

    1992-08-14

    A workshop was held in Corvallis, Oregon on April 9--10, 1992 at the offices of E&S Environmental Chemistry, Inc. The purpose of this workshop was to initiate research efforts on the entitled ``Incorporation of an organic acid representation into MAGIC (Model of Acidification of Groundwater in Catchments) and testing of the revised model using Independent data sources.`` The workshop was attended by a team of internationally-recognized experts in the fields of surface water acid-bass chemistry, organic acids, and watershed modeling. The rationale for the proposed research is based on the recent comparison between MAGIC model hindcasts and paleolimnological inferences of historical acidification for a set of 33 statistically-selected Adirondack lakes. Agreement between diatom-inferred and MAGIC-hindcast lakewater chemistry in the earlier research had been less than satisfactory. Based on preliminary analyses, it was concluded that incorporation of a reasonable organic acid representation into the version of MAGIC used for hindcasting was the logical next step toward improving model agreement.

  2. Utilization of milk amino acids for body gain in suckling mink (Mustela vison) kits

    DEFF Research Database (Denmark)

    Tauson, Anne-Helene; Fink, Rikke; Hansen, Niels E

    2005-01-01

    The efficiency of utilization of milk amino acids for body gain in suckling mink kits from small (n = 3), medium (n = 6) and large litters (n = 9) was investigated by using 36 mink dams and their litters for measurements during lactation weeks 1 through 4. Measurements on each dam and litter were...... performed once, hence three dams per litter size each week (n = 9). Individual milk intake of kits was determined, milk samples were collected and kits were killed for determination of amino acid composition. The most abundant amino acids in milk were glutamate, leucine and aspartate making up about 40......% of total amino acids. Branched chained amino acids made up slightly more than 20% and sulphur containing amino acids less than 5% of total milk amino acids. In kit bodies the sum of glutamate, aspartate and leucine made up about 32% of amino acids, branched chain amino acids about 16% and sulphur...

  3. Coordination of glycerol utilization and clavulanic acid biosynthesis to improve clavulanic acid production in Streptomyces clavuligerus.

    Science.gov (United States)

    Guo, Dekun; Zhao, Youbao; Yang, Keqian

    2013-07-01

    The glycerol utilization (gyl) operon is involved in clavulanic acid (CA) production by Streptomyces clavuligerus, and possibly supplies the glyceraldehyde-3-phosphate (G3P) precursor for CA biosynthesis. The gyl operon is regulated by GylR and is induced by glycerol. To enhance CA production in S. clavuligerus, an extra copy of ccaR expressed from Pgyl (the gyl promoter) was integrated into the chromosome of S. clavuligerus NRRL 3585. This construct coordinated the transcription of CA biosynthetic pathway genes with expression of the gyl operon. In the transformants carrying the Pgyl-controlled regulatory gene ccaR, CA production was enhanced 3.19-fold in glycerol-enriched batch cultures, relative to the control strain carrying an extra copy of ccaR controlled by its own promoter (PccaR). Consistent with enhanced CA production, the transcription levels of ccaR, ceas2 and claR were significantly up-regulated in the transformants containing Pgyl-controlled ccaR.

  4. Differential Utilization of Dietary Fatty Acids in Benign and Malignant Cells of the Prostate.

    Directory of Open Access Journals (Sweden)

    Andrea Dueregger

    Full Text Available Tumor cells adapt via metabolic reprogramming to meet elevated energy demands due to continuous proliferation, for example by switching to alternative energy sources. Nutrients such as glucose, fatty acids, ketone bodies and amino acids may be utilized as preferred substrates to fulfill increased energy requirements. In this study we investigated the metabolic characteristics of benign and cancer cells of the prostate with respect to their utilization of medium chain (MCTs and long chain triglycerides (LCTs under standard and glucose-starved culture conditions by assessing cell viability, glycolytic activity, mitochondrial respiration, the expression of genes encoding key metabolic enzymes as well as mitochondrial mass and mtDNA content. We report that BE prostate cells (RWPE-1 have a higher competence to utilize fatty acids as energy source than PCa cells (LNCaP, ABL, PC3 as shown not only by increased cell viability upon fatty acid supplementation but also by an increased ß-oxidation of fatty acids, although the base-line respiration was 2-fold higher in prostate cancer cells. Moreover, BE RWPE-1 cells were found to compensate for glucose starvation in the presence of fatty acids. Of notice, these findings were confirmed in vivo by showing that PCa tissue has a lower capacity in oxidizing fatty acids than benign prostate. Collectively, these metabolic differences between benign and prostate cancer cells and especially their differential utilization of fatty acids could be exploited to establish novel diagnostic and therapeutic strategies.

  5. GLYCOLIC-FORMIC ACID FLOWSHEET FINAL REPORT FOR DOWNSELECTION DECISION

    Energy Technology Data Exchange (ETDEWEB)

    Lambert, D.; Pickenheim, B.; Stone, M.; Newell, J.; Best, D.

    2011-03-10

    Flowsheet testing was performed to develop the nitric-glycolic-formic acid flowsheet (referred to as the glycolic-formic flowsheet throughout the rest of the report) as an alternative to the nitric/formic flowsheet currently being processed at the DWPF. This new flowsheet has shown that mercury can be removed in the Sludge Receipt and Adjustment Tank (SRAT) with minimal hydrogen generation. All processing objectives were also met, including greatly reducing the Slurry Mix Evaporator (SME) product yield stress as compared to the baseline nitric/formic flowsheet. Forty-six runs were performed in total, including the baseline run and the melter feed preparation runs. Significant results are summarized. The baseline nitric/formic flowsheet run, using the SB6 simulant produced by Harrell was extremely difficult to process successfully under existing DWPF acceptance criteria with this simulant at the HM levels of noble metals. While nitrite was destroyed and mercury was removed to near the DWPF limit, the rheology of the SRAT and SME products were well above design basis and hydrogen generation far exceeded the DWPF SRAT limit. In addition, mixing during the SME cycle was very poor. In this sense, the nitric/glycolic/formic acid flowsheet represents a significant upgrade over the current flowsheet. Mercury was successfully removed with almost no hydrogen generation and the SRAT and SME products yield stresses were within process limits or previously processed ranges. The glycolic-formic flowsheet has a very wide processing window. Testing was completed from 100% to 200% of acid stoichiometry and using a glycolic-formic mixture from 40% to 100% glycolic acid. The testing met all processing requirements throughout these processing windows. This should allow processing at an acid stoichiometry of 100% and a glycolic-formic mixture of 80% glycolic acid with minimal hydrogen generation. It should also allow processing endpoints in the SRAT and SME at significantly higher

  6. Electricity pricing and the financial health of electric utilities in the United States. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1984-08-01

    The electric utility industry in the United States is characterized by wide diversity in utility ownership, management, cost structure, and regulation. There are approxiately 240 investor-owned utilities in the US. There are more than 1900 government utilities in the US. There are more than 50 regulatory commissions which regulate retail prices to ultimate customers. This substantial diversity in regulatory policies, processes, and rules applied to utilities across the country as well as significant regional variations in costs results in a wide variation in electricity prices and financial health among utilities. Prices and financial conditions for the industry as a whole also have varied substantially over time. During the 1960s, utilities were financially healthy, and real electricity prices were falling. In the decade following the Arab oil embargo in 1973, however, the industry was characterized by poor financial health and rapidly rising prices, although the reasons for these conditions varied somewhat between the first part of the decade and the last few years (1980-82) of the decade. Improved economic conditions during 1983 have contributed to improved utility financial health. A continuation of these economic conditions would result in continued financial stability for most utilties. Some individual utilities - particularly those with large constuction programs and those facing substantial reductions in the demand for electricity - may continue to exhibit financial problems. Utilities that have attempted to complete large nuclear projects during the presence of sharply declining load growth face severe financial problems. Prospects for the future financial health of the industry depend critically on the future demand for electricity, requirements for new generating capacity and other utility investments, and resolution of several policy issues affecting utility ratemaking, operations, and financial accounting.

  7. Economic feasibility of alternative utility-sponsored weatherization programs in Maryland. Phase 2. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Dubinsky, R.; Thibodeau, T.

    1983-05-01

    The economic costs and benefits of sponsoring weatherization programs are presented for Maryland utilities that operate Residential Conservation Service (RCS) programs. The key issues investigated are: the potential for cost-effective weatherization in each utility's service area; the savings in fuel and generating capacity that are likely to result from alternative weatherization programs; and the cost-effectiveness of such programs to each utility and to its ratepayers.

  8. Final Report Report: Smart Grid Ready PV Inverters with Utility Communication

    Energy Technology Data Exchange (ETDEWEB)

    Seal, Brian [Electric Power Research Inst. (EPRI), Knovville, TN (United States); Huque, Aminul [Electric Power Research Inst. (EPRI), Knovville, TN (United States); Rogers, Lindsey [Electric Power Research Inst. (EPRI), Knovville, TN (United States); Key, Tom [Electric Power Research Inst. (EPRI), Knovville, TN (United States); Riley, Cameron [Electric Power Research Inst. (EPRI), Knovville, TN (United States); Li, Huijuan [Electric Power Research Inst. (EPRI), Knovville, TN (United States); York, Ben [Electric Power Research Inst. (EPRI), Knovville, TN (United States); Purcell, Chris [BPL Global, Inc., Canonsburg, PA (United States); Pacific, Oliver [Spirae, Inc., Fort Collins, CO (United States); Ropp, Michael [Northern Plains Power Technologies, Brookings, SD (United States); Tran, Teresa [DTE Energy, Detroit, MI (United States); Asgeirsson, Hawk [DTE Energy, Detroit, MI (United States); Woodard, Justin [National Grid, Warwick (United Kingdom); Steffel, Steve [Pepco Holdings, Inc., Washington, DC (United States)

    2016-03-30

    In 2011, EPRI began a four-year effort under the Department of Energy (DOE) SunShot Initiative Solar Energy Grid Integration Systems - Advanced Concepts (SEGIS-AC) to demonstrate smart grid ready inverters with utility communication. The objective of the project was to successfully implement and demonstrate effective utilization of inverters with grid support functionality to capture the full value of distributed photovoltaic (PV). The project leveraged ongoing investments and expanded PV inverter capabilities, to enable grid operators to better utilize these grid assets. Developing and implementing key elements of PV inverter grid support capabilities will increase the distribution system’s capacity for higher penetration levels of PV, while reducing the cost. The project team included EPRI, Yaskawa-Solectria Solar, Spirae, BPL Global, DTE Energy, National Grid, Pepco, EDD, NPPT and NREL. The project was divided into three phases: development, deployment, and demonstration. Within each phase, the key areas included: head-end communications for Distributed Energy Resources (DER) at the utility operations center; methods for coordinating DER with existing distribution equipment; back-end PV plant master controller; and inverters with smart-grid functionality. Four demonstration sites were chosen in three regions of the United States with different types of utility operating systems and implementations of utility-scale PV inverters. This report summarizes the project and findings from field demonstration at three utility sites.

  9. Industrial applications study. Volume II. Industrial data base. Final report. [Waste heat recovery and utilization

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Harry L.; Hamel, Bernard B.; Karamchetty, Som; Steigelmann, William H.; Gajanana, Birur C.; Agarwal, Anil P.; Klock, Lawrence W.; Henderson, James M.; Calobrisi, Gary; Hedman, Bruce A.; Koluch, Michael; Biancardi, Frank; Bass, Robert; Landerman, Abraham; Peters, George; Limaye, Dilip; Price, Jeffrey; Farr, Janet

    1976-12-01

    An initial evaluation of the waste heat recovery and utilization potential in the manufacturing portion of the industrial sector is presented. The scope of this initial phase addressed the feasibility of obtaining in-depth energy information in the industrial sector. Within this phase, the methodology and approaches for data gathering and assessment were established. Using these approaches, energy use and waste heat profiles were developed at the 2-digit level; with this data, waste heat utilization technologies were evaluated.

  10. Legal-institutional arrangements facilitating offshore wind energy conversion systems (WECS) utilization. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Mayo, L.H.

    1977-09-01

    Concern for the continuing sufficiency of energy supplies in the U.S. has tended to direct increasing attention to unconventional sources of supply, including wind energy. Some of the more striking proposals for the utilization of wind energy relate to offshore configurations. The legal-institutional arrangements for facilitating the utilization of offshore wind energy conversion systems (WECS) are examined by positioning three program alternatives and analyzing the institutional support required for the implementation of each.

  11. Beneficial Effect of Acetic Acid on the Xylose Utilization and Bacterial Cellulose Production by Gluconacetobacter xylinus.

    Science.gov (United States)

    Yang, Xiao-Yan; Huang, Chao; Guo, Hai-Jun; Xiong, Lian; Luo, Jun; Wang, Bo; Chen, Xue-Fang; Lin, Xiao-Qing; Chen, Xin-De

    2014-09-01

    In this work, acetic acid was found as one promising substrate to improve xylose utilization by Gluconacetobacter xylinus CH001. Also, with the help of adding acetic acid into medium, the bacterial cellulose (BC) production by G. xylinus was increased significantly. In the medium containing 3 g l(-1) acetic acid, the optimal xylose concentration for BC production was 20 g l(-1). In the medium containing 20 g l(-1) xylose, the xylose utilization and BC production by G. xylinus were stimulated by acetic acid within certain concentration. The highest BC yield (1.35 ± 0.06 g l(-1)) was obtained in the medium containing 20 g l(-1) xylose and 3 g l(-1) acetic acid after 14 days. This value was 6.17-fold higher than the yield (0.21 ± 0.01 g l(-1)) in the medium only containing 20 g l(-1) xylose. The results analyzed by FE-SEM, FTIR, and XRD showed that acetic acid affected little on the microscopic morphology and physicochemical characteristics of BC. Base on the phenomenon observed, lignocellulosic acid hydrolysates (xylose and acetic acid are main carbon sources present in it) could be considered as one potential substrate for BC production.

  12. Assessment of the potential of solar thermal small power systems in small utilities. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Steitz, P.; Mayo, L.G.; Perkins, S.P. Jr.

    1978-11-01

    This study involved an assessment of the potential economic benefit of small solar thermal electric power systems to small municipal and rural electric utilities. Five different solar thermal small power system configurations were considered in the study representing three different solar thermal technologies. The configurations included: (1) 1-MW, 2-MW, and 10-MW parabolic dish concentrators with a 15-kW heat engine mounted at the focal point of each dish. These systems utilized advanced battery energy storage. (2) A 10-MW system with variable slat concentrators and central steam Rankine energy conversion. This system utilized sensible thermal energy storage. (3) A 50-MW central receiver system consisting of a field of heliostats concentrating energy on a tower-mounted receiver and a central steam Rankine conversion system. This system also utilized sensible thermal storage. The approach used in determining the potential for solar thermal small power systems in the small utility market involved a comparison of the economics of power supply expansion plans for seven hypothetical small utilities through the year 2000 both with and without the solar thermal small power systems. Insolation typical of the Southwestern US was assumed. A comparison of the break-even capital costs with the range of plant costs estimated in this study yields the following conclusions: (1) The parabolic dish concentrator systems could be economically competitive with conventional generation if the lowest capital costs can be achieved. (2) The variable slat concentrator and central receiver systems would have to achieve lower costs than the lowest in the cost ranges generally assumed in the study to become economically competitive. (3) All of the solar thermal plant types are potentially more competitive in utilities which are heavily dependent upon oil.

  13. A novel sialic acid utilization and uptake system in the periodontal pathogen Tannerella forsythia.

    Science.gov (United States)

    Roy, Sumita; Douglas, C W Ian; Stafford, Graham P

    2010-05-01

    Tannerella forsythia is a key contributor to periodontitis, but little is known of its virulence mechanisms. In this study we have investigated the role of sialic acid in biofilm growth of this periodontal pathogen. Our data show that biofilm growth of T. forsythia is stimulated by sialic acid, glycolyl sialic acid, and sialyllactose, all three of which are common sugar moieties on a range of important host glycoproteins. We have also established that growth on sialyllactose is dependent on the sialidase of T. forsythia since the sialidase inhibitor oseltamivir suppresses growth on sialyllactose. The genome of T. forsythia contains a sialic acid utilization locus, which also encodes a putative inner membrane sialic acid permease (NanT), and we have shown this is functional when it is expressed in Escherichia coli. This genomic locus also contains a putatively novel TonB-dependent outer membrane sialic acid transport system (TF0033-TF0034). In complementation studies using an Escherichia coli strain devoid of its outer membrane sialic acid transporters, the cloning and expression of the TF0033-TF0034 genes enabled an E. coli nanR nanC ompR strain to utilize sialic acid as the sole carbon and energy source. We have thus identified a novel sialic acid uptake system that couples an inner membrane permease with a TonB-dependent outer membrane transporter, and we propose to rename these novel sialic acid uptake genes nanO and nanU, respectively. Taken together, these data indicate that sialic acid is a key growth factor for this little-characterized oral pathogen and may be key to its physiology in vivo.

  14. Industrial applications study. Volume IV. Industrial plant surveys. Final report. [Waste heat recovery and utilization

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Harry L.; Hamel, Bernard B.; Karamchetty, Som; Steigelmann, William H.; Gajanana, Birur C.; Agarwal, Anil P.; Klock, Lawrence M.; Henderson, James M.; Calobrisi, Gary; Hedman, Bruce A.; Koluch, Michael; Biancardi, Frank; Bass, Robert; Landerman, Abraham; Peters, George; Limaye, Dilip; Price, Jeffrey; Farr, Janet

    1977-01-01

    An initial evaluation of the waste heat recovery and utilization potential in the manufacturing portion of the industrial sector is presented. The scope of this initial phase addressed the feasibility of obtaining in-depth energy information in the industrial sector. Within this phase, the methodology and approaches for data gathering and assessment were established. Using these approaches, energy use and waste heat profiles were developed at the 2-digit level; with this data, waste heat utilization technologies were evaluated. This study represents an important first step in the evaluation of waste heat recovery potential.

  15. Mode of utilization of amino acids as growth substrates by Azospirillum brasilense.

    Science.gov (United States)

    Bhattacharya, Pradip

    2005-12-01

    The study was undertaken to analyze the rate of uptake and utilization of various amino acids by Azospirillum brasilense Sp81 (RG) in a basal mineral salts solution under non-nitrogen fixing condition. These amino acids including other nitrogenous compounds were tested for both N- and C-sources. The kinetic constants (Km and Vmax) of uptake of some amino acids (e.g. lysine, arginine, proline, glutamine and glutamic acid) were exploited using a Hanes-Woolf plot, and discussed in the context of nitrogen starvation or both carbon and nitrogen starvation. To summarize all the kinetic data for these amino acids strongly suggested that the mode of these amino acids utilization in this bacterium followed the same general pattern, although the quantitative differences were there. A single amino acid was able to satisfy the nitrogen needs of this bacterium in basal mineral salts solution, and this possibility could be considered for the cost-effective growth medium for this bacterium in the biotechnological industry.

  16. 77 FR 75978 - Utility Scale Wind Towers From the People's Republic of China: Final Affirmative Countervailing...

    Science.gov (United States)

    2012-12-26

    ...., Ltd. and its affiliates (collectively, CS Wind) and Titan Wind Energy (Suzhou) Co., Ltd. and its affiliates (collectively, the Titan Companies). \\1\\ See Utility Scale Wind Towers From the People's Republic... nacelle generator, interior lighting, tool and storage lockers) attached to the wind tower...

  17. National Utility Financial Statement model (NUFS). Volume III of III: software description. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1981-10-29

    This volume contains a description of the software comprising the National Utility Financial Statement Model (NUFS). This is the third of three volumes describing NUFS provided by ICF Incorporated under contract DEAC-01-79EI-10579. The three volumes are entitled: model overview and description, user's guide, and software guide.

  18. Hawaii Utility Integration Initiatives to Enable Wind (Wind HUI) Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Dora Nakafuji; Lisa Dangelmaier; Chris Reynolds

    2012-07-15

    To advance the state and nation toward clean energy, Hawaii is pursuing an aggressive Renewable Portfolio Standard (RPS), 40% renewable generation and 30% energy efficiency and transportation initiatives by 2030. Additionally, with support from federal, state and industry leadership, the Hawaii Clean Energy Initiative (HCEI) is focused on reducing Hawaii's carbon footprint and global warming impacts. To keep pace with the policy momentum and changing industry technologies, the Hawaiian Electric Companies are proactively pursuing a number of potential system upgrade initiatives to better manage variable resources like wind, solar and demand-side and distributed generation alternatives (i.e. DSM, DG). As variable technologies will continue to play a significant role in powering the future grid, practical strategies for utility integration are needed. Hawaiian utilities are already contending with some of the highest penetrations of renewables in the nation in both large-scale and distributed technologies. With island grids supporting a diverse renewable generation portfolio at penetration levels surpassing 40%, the Hawaiian utilities experiences can offer unique perspective on practical integration strategies. Efforts pursued in this industry and federal collaborative project tackled challenging issues facing the electric power industry around the world. Based on interactions with a number of western utilities and building on decades of national and international renewable integration experiences, three priority initiatives were targeted by Hawaiian utilities to accelerate integration and management of variable renewables for the islands. The three initiatives included: Initiative 1: Enabling reliable, real-time wind forecasting for operations by improving short-term wind forecasting and ramp event modeling capabilities with local site, field monitoring; Initiative 2: Improving operators situational awareness to variable resources via real-time grid condition

  19. Hawaii Utility Integration Initiatives to Enable Wind (Wind HUI) Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Dora Nakafuji; Lisa Dangelmaier; Chris Reynolds

    2012-07-15

    To advance the state and nation toward clean energy, Hawaii is pursuing an aggressive Renewable Portfolio Standard (RPS), 40% renewable generation and 30% energy efficiency and transportation initiatives by 2030. Additionally, with support from federal, state and industry leadership, the Hawaii Clean Energy Initiative (HCEI) is focused on reducing Hawaii's carbon footprint and global warming impacts. To keep pace with the policy momentum and changing industry technologies, the Hawaiian Electric Companies are proactively pursuing a number of potential system upgrade initiatives to better manage variable resources like wind, solar and demand-side and distributed generation alternatives (i.e. DSM, DG). As variable technologies will continue to play a significant role in powering the future grid, practical strategies for utility integration are needed. Hawaiian utilities are already contending with some of the highest penetrations of renewables in the nation in both large-scale and distributed technologies. With island grids supporting a diverse renewable generation portfolio at penetration levels surpassing 40%, the Hawaiian utilities experiences can offer unique perspective on practical integration strategies. Efforts pursued in this industry and federal collaborative project tackled challenging issues facing the electric power industry around the world. Based on interactions with a number of western utilities and building on decades of national and international renewable integration experiences, three priority initiatives were targeted by Hawaiian utilities to accelerate integration and management of variable renewables for the islands. The three initiatives included: Initiative 1: Enabling reliable, real-time wind forecasting for operations by improving short-term wind forecasting and ramp event modeling capabilities with local site, field monitoring; Initiative 2: Improving operators situational awareness to variable resources via real-time grid condition

  20. Electric utility engineer`s FGD manual -- Volume 1: FGD process design. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-04

    Part 1 of the Electric Utility Engineer`s Flue Gas Desulfurization (FGD) Manual emphasizes the chemical and physical processes that form the basis for design and operation of lime- and limestone-based FGD systems applied to coal- or oil-fired steam electric generating stations. The objectives of Part 1 are: to provide a description of the chemical and physical design basis for lime- and limestone-based wet FGD systems; to identify and discuss the various process design parameters and process options that must be considered in developing a specification for a new FGD system; and to provide utility engineers with process knowledge useful for operating and optimizing a lime- or limestone-based wet FGD system.

  1. Agribusiness geothermal energy utilization potential of Klamath and Western Snake River Basins, Oregon. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Lienau, P.J.

    1978-03-01

    Resource assessment and methods of direct utilization for existing and prospective food processing plants have been determined in two geothermal resource areas in Oregon. Ore-Ida Foods, Inc. and Amalgamated Sugar Company in the Snake River Basin; Western Polymer Corporation (potato starch extraction) and three prospective industries--vegetable dehydration, alfalfa drying and greenhouses--in the Klamath Basin have been analyzed for direct utilization of geothermal fluids. Existing geologic knowledge has been integrated to indicate locations, depth, quality, and estimated productivity of the geothermal reservoirs. Energy-economic needs and balances, along with cost and energy savings associated with field development, delivery systems, in-plant applications and fluid disposal have been calculated for interested industrial representatives.

  2. C-Myc induced compensated cardiac hypertrophy increases free fatty acid utilization for the citric acid cycle.

    Science.gov (United States)

    Olson, Aaron K; Ledee, Dolena; Iwamoto, Kate; Kajimoto, Masaki; O'Kelly Priddy, Colleen; Isern, Nancy; Portman, Michael A

    2013-02-01

    The protooncogene C-Myc (Myc) regulates cardiac hypertrophy. Myc promotes compensated cardiac function, suggesting that the operative mechanisms differ from those leading to heart failure. Myc regulation of substrate metabolism is a reasonable target, as Myc alters metabolism in other tissues. We hypothesize that Myc induced shifts in substrate utilization signal and promote compensated hypertrophy. We used cardiac specific Myc-inducible C57/BL6 male mice between 4-6 months old that develop hypertrophy with tamoxifen (tam) injections. Isolated working hearts and (13)Carbon ((13)C)-NMR were used to measure function and fractional contributions (Fc) to the citric acid cycle by using perfusate containing (13)C-labeled free fatty acids, acetoacetate, lactate, unlabeled glucose and insulin. Studies were performed at pre-hypertrophy (3-days tam, 3dMyc), established hypertrophy (7-days tam, 7dMyc) or vehicle control (Cont). Non-transgenic siblings (NTG) received 7-days tam or vehicle to assess drug effect. Hypertrophy was assessed by echocardiograms and heart weights. Western blots were performed on key metabolic enzymes. Hypertrophy occurred in 7dMyc only. Cardiac function did not differ between groups. Tam alone did not affect substrate contributions in NTG. Substrate utilization was not significantly altered in 3dMyc versus Cont. The free fatty acid FC was significantly greater in 7dMyc versus Cont with decreased unlabeled Fc, which is predominately exogenous glucose. Free fatty acid flux to the citric acid cycle increased while lactate flux was diminished in 7dMyc compared to Cont. Total protein levels of a panel of key metabolic enzymes were unchanged; however total protein O-GlcNAcylation was increased in 7dMyc. Substrate utilization changes for the citric acid cycle did not precede hypertrophy; therefore they are not the primary signal for cardiac growth in this model. Free fatty acid utilization and oxidation increase at established hypertrophy. Understanding the

  3. Used frying oils and fats and their utilization in the production of methyl esters of higher fatty acids

    Energy Technology Data Exchange (ETDEWEB)

    Cvengros, J. Jan; Cvengrosova, Zuzana [Slovak Univ. of Technology, Faculty of Chemical and Food Technology, Bratislava (Slovakia)

    2004-08-01

    From the point of view price and available capacity used frying oils or fats (UFO) represent an attractive raw material for the production of methyl esters (ME) of higher fatty acids as alternative fuels for diesel engines. If they are treated such that the required quality, with an acidity number up to 3.0 mg KOH/g and a water content up to 0.1 wt%, is achieved they can be processed to ME using standard techniques of alkali-catalysed transesterification with methanol which are utilized for production of the ME from new oils/fats. The problematic waste can thus be converted to an ecologically friendly fuel. Vacuum distillation of free fatty acids in a film evaporator is an effective method for simultaneously decreasing the content of FFA and water in UFO. Final distillation of raw ME in a film vacuum evaporator results in practically all parameters required by the standard, in the final ME being achieved. Undesirable low-temperature properties of ME derived from UFO, due to higher fraction of saturated acyls, can be adjusted by the addition of depressants-flow improvers for winterization. Some simplified methods for the quality control of UFO and ME are discussed. The conversion of acylglycerols to ME is monitored by GLC with a packed column, where the peak areas of ME in the sample before and after the reaction with an effective methylation agent are compared. The method for the determination of the water content in esters utilizes the reaction of calcium carbide with water, the volume of acetylene being measured. (Author)

  4. A metabolic-based approach to improve xylose utilization for fumaric acid production from acid pretreated wheat bran by Rhizopus oryzae.

    Science.gov (United States)

    Wang, Guanyi; Huang, Di; Li, Yong; Wen, Jianping; Jia, Xiaoqiang

    2015-03-01

    In this work, wheat bran (WB) was utilized as feedstock to synthesize fumaric acid by Rhizopus oryzae. Firstly, the pretreatment process of WB by dilute sulfuric acid hydrolysis undertaken at 100°C for 30min offered the best performance for fumaric acid production. Subsequently, through optimizing the seed culture medium, a suitable morphology (0.55mm pellets diameter) of R. oryzae was obtained. Furthermore, a metabolic-based approach was developed to profile the differences of intracellular metabolites concentration of R. oryzae between xylose (the abundant sugar in wheat bran hydrolysate (WBH)) and glucose metabolism. The xylitol, sedoheptulose 7-phosphate, ribulose 5-phosphate, glucose 6-phosphate, proline and serine were responsible for fumaric acid biosynthesis limitation in xylose fermentation. Consequently, regulation strategies were proposed, leading to a 149% increase in titer (up to 15.4g/L). Finally, by combinatorial regulation strategies the highest production was 20.2g/L from WBH, 477% higher than that of initial medium.

  5. Final Technical Report Laramie County Community College: Utility-Scale Wind Energy Technology

    Energy Technology Data Exchange (ETDEWEB)

    Douglas P. Cook

    2012-05-22

    The Utility-Scale Wind Energy Technology U.S. Department of Energy (DOE) grant EE0000538, provided a way ahead for Laramie County Community College (LCCC) to increase educational and training opportunities for students seeking an Associate of Applied Science (AAS) or Associate of Science (AS) degree in Wind Energy Technology. The DOE grant enabled LCCC to program, schedule, and successfully operate multiple wind energy technology cohorts of up to 20-14 students per cohort simultaneously. As of this report, LCCC currently runs four cohorts. In addition, the DOE grant allowed LCCC to procure specialized LABVOLT electronic equipment that directly supports is wind energy technology curriculum.

  6. C-Myc Induced Compensated Cardiac Hypertrophy Increases Free Fatty Acid Utilization for the Citric Acid Cycle

    Energy Technology Data Exchange (ETDEWEB)

    Olson, Aaron; Ledee, Dolena; Iwamoto, Kate; Kajimoto, Masaki; O' Kelly-Priddy, Colleen M.; Isern, Nancy G.; Portman, Michael A.

    2013-02-01

    The protooncogene C-Myc (Myc) regulates cardiac hypertrophy. Myc promotes compensated cardiac function, suggesting that the operative mechanisms differ from those leading to heart failure. Myc regulation of substrate metabolism is a reasonable target, as Myc alters metabolism in other tissues. We hypothesize that Myc-induced shifts in substrate utilization signal and promote compensated hypertrophy. We used cardiac specific Myc-inducible C57/BL6 male mice between 4-6 months old that develop hypertrophy with tamoxifen (tam). Isolated working hearts and 13Carbon (13C )-NMR were used to measure function and fractional contributions (Fc) to the citric acid cycle by using perfusate containing 13C-labeled free fatty acids, acetoacetate, lactate, unlabeled glucose and insulin. Studies were performed at pre-hypertrophy (3-days tam, 3dMyc), established hypertrophy (7-days tam, 7dMyc) or vehicle control (cont). Non-transgenic siblings (NTG) received 7-days tam or vehicle to assess drug effect. Hypertrophy was confirmed by echocardiograms and heart weights. Western blots were performed on key metabolic enzymes. Hypertrophy occurred in 7dMyc only. Cardiac function did not differ between groups. Tam alone did not affect substrate contribution in NTG. Substrate utilization was not significantly altered in 3dMyc versus cont. The free fatty acid FC was significantly greater in 7dMyc vs cont with decreased unlabeled Fc, which is predominately exogenous glucose. Free fatty acid flux to the citric acid cycle increased while lactate flux was diminished in 7dMyc compared to cont. Total protein levels of a panel of key metabolic enzymes were unchanged; however total protein O-GlcNAcylation was increased in 7dMyc. Substrate utilization changes did not precede hypertrophy; therefore they are not the primary signal for cardiac growth in this model. Free fatty acid utilization and oxidation increase at established hypertrophy. Understanding the mechanisms whereby this change maintained

  7. The market potential for SMES in electric utility applications. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1994-06-01

    Superconducting magnetic energy storage (SMES) is an emerging technology with features that are potentially attractive in electric utility applications. This study evaluates the potential for SMES technology in the generation, transmission, distribution, and use of electric energy; the time frame of the assessment is through the year 2030. Comparisons are made with other technology options, including both commercially available and advanced systems such as various peaking generation technologies, transmission stability improvement technologies, and power quality enhancement devices. The methodology used for this study focused on the needs of the market place, the capabilities of S and the characteristics of the competing technologies. There is widespread interest within utilities for the development of SMES technology, but there is no general consensus regarding the most attractive size. Considerable uncertainty exists regarding the eventual costs and benefits of commercial SMES systems, but general trends have been developed based on current industry knowledge. Results of this analysis indicate that as storage capacity increases, cost increases at a rate faster than benefits. Transmission system applications requiring dynamic storage appear to have the most attractive economics. Customer service applications may be economic in the near term, but improved ride-through capability of end-use equipment may limit the size of this market over time. Other applications requiring greater storage capacity appear to be only marginally economic at best.

  8. [Advances in studies of absorption and utilization of amino acids by plants: A review].

    Science.gov (United States)

    Cao, Xiao-chuang; Wu, Liang-huan; Ma, Qing-xu; Jin, Qian-yu

    2015-03-01

    Plant can directly take up the intact amino acids, thus bypass the microbial mineralization of organic nitrogen. As an excellent carbon and nitrogen source, there exists competition for amino acid absorption between plant roots.and soil microorganisms. And the total flux of amino acids in soil may be enormous due to the extensive sources and short half-life. Studies on amino acid nitrogen nutritional contribution for plant by the technique of nitrogen isotopic tracer, has become a research topic in recent years ,which will help us better understand the principle of soil fertility. This paper summarized the recent researches on amino acid morphological characteristics in soil and its metabolic mechanism and nitrogen nutritional contribution for plant in different ecosystems, and discussed the present status and development trend of the amino acid circulation mechanism in the plant-soil-microorganism ecosystem and its bioavailability for plant. Finally, the topics of environmental regulating mechanism of amino acid bioavailability, amino acid carbon-nitrogen metabolism, and how to improve the field organic nitrogen management were all the core issues to be resolved.

  9. AMPKα is critical for enhancing skeletal muscle fatty acid utilization during in vivo exercise in mice

    DEFF Research Database (Denmark)

    Fentz, Joachim; Kjøbsted, Rasmus; Birk, Jesper B.;

    2015-01-01

    The importance of AMPK in regulation of fatty acid (FA) oxidation in skeletal muscle with contraction/exercise is unresolved. Using a mouse model lacking both AMPKα1 and -α2 in skeletal muscle specifically (mdKO), we hypothesized that FA utilization would be impaired in skeletal muscle. AMPKα md......) and FABPpm (plasma membrane fatty acid binding protein) (by ∼17-40%), together with fully abolished TBC1D1 (tre-2/USP6, BUB2, cdc16 domain family member 1) Ser(237) phosphorylation during contraction/exercise in AMPKα mdKO mice, may impair FA transport capacity and FA transport protein translocation...

  10. Region-specific study of the electric utility industry. Phase I, final report

    Energy Technology Data Exchange (ETDEWEB)

    Wacaster, A.J. (ed.)

    1985-07-01

    This report describes the financial background of the electric utility industry in VACAR, reports on the present condition of the industry and then assesses the future of this industry. The Virginia-Carolinas subregion (VACAR) of the Southeastern Electric Reliability Council (SERC) was selected for this regional study because of its cooperativeness and its representative mix of powerplants, for example coal, hydro, nuclear, oil. It was found that the supply of future economic electricity is in jeopardy because of the regulatory process, the increasing risk associated with large scale generating stations and the weakening of the nuclear option. A number of options for the future were considered, including deregulation, government ownership and retaining the present system with modifications. The option selected to improve the present condition of the electricity industry was to make the present system work. The present system is sound, and with modifications, problems could be solved within the existing framework. 8 figs., 4 tabs.

  11. Mathematical model of a utility firm. Final technical report, Part I

    Energy Technology Data Exchange (ETDEWEB)

    1983-08-21

    Utility companies are in the predicament of having to make forecasts, and draw up plans for the future, in an increasingly fluid and volatile socio-economic environment. The project being reported is to contribute to an understanding of the economic and behavioral processes that take place within a firm, and without it. Three main topics are treated. One is the representation of the characteristics of the members of an organization, to the extent to which characteristics seem pertinent to the processes of interest. The second is the appropriate management of the processes of change by an organization. The third deals with the competitive striving towards an economic equilibrium among the members of a society in the large, on the theory that this process might be modeled in a way which is similar to the one for the intra-organizational ones. This volume covers mainly the first topic.

  12. Sex Differences in Long Chain Fatty Acid Utilization and Fatty Acid Binding Protein Concentration in Rat Liver

    Science.gov (United States)

    Ockner, Robert K.; Burnett, David A.; Lysenko, Nina; Manning, Joan A.

    1979-01-01

    Female sex and estrogen administration are associated with increased hepatic production of triglyceride-rich lipoproteins; the basis for this has not been fully elucidated. Inasmuch as hepatic lipoprotein production is also influenced by FFA availability and triglyceride biosynthesis, we investigated sex differences in FFA utilization in rat hepatocyte suspensions and in the components of the triglyceride biosynthetic pathway. Isolated adult rat hepatocyte suspensions were incubated with albumin-bound [14C]oleate for up to 15 min. At physiological and low oleate concentrations, cells from females incorporated significantly more 14C into glycerolipids, especially triglycerides, and into oxidation products than did male cells, per milligram cell protein. At 0.44 mM oleate, incorporation into triglycerides in female cells was approximately twice that in male cells. Comparable sex differences were observed in cells from fasted animals and when [14C]-glycerol incorporation was measured. At higher oleate concentrations, i.e., fatty acid:albumin mole ratios in excess of 2:1, these sex differences were no longer demonstrable, suggesting that maximal rates of fatty acid esterification and oxidation were similar in female and male cells. In female and male hepatic microsomes, specific activities of long chain acyl coenzyme A synthetase, phosphatidate phosphohydrolase, and diglyceride acyltransferase were similar, but glycerol-3-phosphate acyltransferase activity was slightly greater in females at certain substrate concentrations. Microsomal incorporation of [14C]oleate into total glycerolipids was not significantly greater in females. In further contrast to intact cells, microsomal incorporation of [14C]oleate into triglycerides, although significantly greater in female microsomes, accounted for only a small fraction of the fatty acid esterified. The binding affinity and stoichiometry of partially purified female hepatic fatty acid binding protein (FABP) were similar to

  13. Testing and evaluation of an alcohol production facility utilizing potatoes as a feedstock. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Kuby, W.; Nackord, S.; Wyss, W.

    1984-05-01

    This study presents the sampling and analysis results for the characterization of liquid effluents and solid residuals from a culled potato feedstock process for the production of ethanol for use as fuel. The facility tested produces approximately 1 million gallons per year of ethanol and is located in eastern Idaho. Liquid and solid samples were taken throughout the process from the following locations: sluice/flume water, chopper product, makeup water, cooker product, fermenter product, beer tank, stillage, interim and final product, washwater, fuel oil, bath and 'Sparkle' bath. Analytical results for the ethanol plant effluents include: ethanol and sugar content, conventional parameters, metals, cyanide, phenols, nutrients, oil and grease, priority pollutant organics, and selected pesticides. The most significant characteristics of concern were the BOD and COD levels.

  14. Financial constraints in capacity planning: a national utility regulatory model (NUREG). Volume I of III: methodology. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1981-10-29

    This report develops and demonstrates the methodology for the National Utility Regulatory (NUREG) Model developed under contract number DEAC-01-79EI-10579. It is accompanied by two supporting volumes. Volume II is a user's guide for operation of the NUREG software. This includes description of the flow of software and data, as well as the formats of all user data files. Finally, Volume III is a software description guide. It briefly describes, and gives a listing of, each program used in NUREG.

  15. Tire Development for Effective Transportation and Utilization of Used Tires, CRADA 01-N044, Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Susan M. Maley

    2004-03-31

    Scrap tires represent a significant disposal and recycling challenge for the United States. Over 280 million tires are generated on an annual basis, and several states have large stockpiles or abandoned tire piles that are slated for remediation. While most states have programs to address the accumulation and generation of scrap tires, most of these states struggle with creating and sustaining recycling or beneficial end use markets. One of the major issues with market development has been the costs associated with transporting and processing the tires into material for recycling or disposal. According to a report by the Rubber Manufactures Association tire-derived fuel (TDF) represents the largest market for scrap tires, and approximately 115 million tires were consumed in 2001 as TDF (U.S. Scrap Tire Markets, 2001, December 2002, www.rma.org/scraptires). This market is supported primarily by cement kilns, followed by various industries including companies that operate utility and industrial boilers. However the use of TDF has not increased and the amount of TDF used by boiler operators has declined. The work completed through this cooperative research and development agreement (CRADA) has shown the potential of a mobile tire shredding unit to economically produce TDF and to provide an alterative low cost fuel to suitable coal-fired power systems. This novel system addresses the economic barriers by processing the tires at the retailer, thereby eliminating the costs associated with hauling whole tires. The equipment incorporated into the design allow for small 1-inch chunks of TDF to be produced in a timely fashion. The TDF can then be co-fired with coal in suitable combustion systems, such as a fluidized bed. Proper use of TDF has been shown to boost efficiency and reduce emissions from power generation systems, which is beneficial to coal utilization in existing power plants. Since the original scope of work outlined in the CRADA could not be completed because

  16. Utility of lab-on-a-chip technology for high-throughput nucleic acid and protein analysis

    DEFF Research Database (Denmark)

    Hawtin, Paul; Hardern, Ian; Wittig, Rainer

    2005-01-01

    On-chip electrophoresis can provide size separations of nucleic acids and proteins similar to more traditional slab gel electrophoresis. Lab-on-a-chip (LoaC) systems utilize on-chip electrophoresis in conjunction with sizing calibration, sensitive detection schemes, and sophisticated data analysis...... to achieve rapid analysis times (C systems to enable and augment systems biology investigations. RNA quality, as assessed by an RNA integrity number score, is compared to existing quality control (QC) measurements. High-throughput DNA analysis of multiplex PCR...... samples is used to stratify gene sets for disease discovery. Finally, the applicability of a high-throughput LoaC system for assessing protein purification is demonstrated. The improvements in workflow processes, speed of analysis, data accuracy and reproducibility, and automated data analysis...

  17. Low-maintenance, valve-regulated, lead/acid batteries in utility applications

    Science.gov (United States)

    Cook, G. M.; Spindler, W. C.

    Electric power utility companies have various needs for lead/acid batteries, and also are beginning to promote customer-side-of-the meter applications for mutual benefits. Increasing use of lead/acid batteries in the future will depend heavily on improving performance and reliability of sealed, recombination designs, and on their versatility for many applications. Classifying various utility uses could be by cycling requirements, depth-of-discharge, power or energy (ratio of watts to hours), or by site (utility or customer). Deep-cycling examples are energy storage, peak-shaving and electric vehicles. Shallow-cycling examples are frequency regulation and reactive power control. Infrequent discharge examples are stationary service and spinning reserve. (Float service for telecommunications and uninterruptible power sources (UPS) applications are not addressed.) Some present and planned installations of valve-regulated lead/acid batteries are surveyed. Performance characteristics will be discussed, including recent results of testing both gel and absorptive glass mat (AGM) types of deep-cycling batteries. Recommendations for future research and development of valve-regulated cell technology are outlined, based on a recent conference organized by the United States Department of Energy (USDOE) and the Electric Power Research Institute (EPRI).

  18. Hot dry rock geothermal energy for U.S. electric utilities. Draft final report

    Energy Technology Data Exchange (ETDEWEB)

    1993-06-01

    In order to bring an electric utility component into the study of hot dry rock geothermal energy called for in the Energy Policy Act of 1992 (EPAct), EPRI organized a one-day conference in Philadelphia on January 14,1993. The conference was planned as the first day of a two-day sequence, by coordinating with the U.S. Geological Survey (USGS) and the U.S. Department of Energy (DOE). These two federal agencies were charged under EPAct with the development of a report on the potential for hot dry rock geothermal energy production in the US, especially the eastern US. The USGS was given lead responsibility for a report to be done in association with DOE. The EPRI conference emphasized first the status of technology development and testing in the U.S. and abroad, i.e., in western Europe, Russia and Japan. The conference went on to address the extent of knowledge regarding the resource base in the US, especially in the eastern half of the country, and then to address some practical business aspects of organizing projects or industries that could bring these resources into use, either for thermal applications or for electric power generation.

  19. Cesium removal demonstration utilizing crystalline silicotitanate sorbent for processing Melton Valley Storage Tank supernate: Final report

    Energy Technology Data Exchange (ETDEWEB)

    Walker, J.F. Jr.; Taylor, P.A.; Cummins, R.L. [and others

    1998-03-01

    This report provides details of the Cesium Removal Demonstration (CsRD), which was conducted at Oak Ridge National Laboratory (ORNL) on radioactive waste from the Melton Valley Storage Tanks. The CsRD was the first large-scale use of state-of-the-art sorbents being developed by private industry for the selective removal of cesium and other radionuclides from liquid wastes stored across the DOE complex. The crystalline silicotitanate sorbent used in the demonstration was chosen because of its effectiveness in laboratory tests using bench-scale columns. The demonstration showed that the cesium could be removed from the supernate and concentrated on a small-volume, solid waste form that would meet the waste acceptance criteria for the Nevada Test Site. During this project, the CsRD system processed > 115,000 L (30,000 gal) of radioactive supernate with minimal operational problems. Sluicing, drying, and remote transportation of the sorbent, which could not be done on a bench scale, were successfully demonstrated. The system was then decontaminated to the extent that it could be contact maintained with the use of localized shielding only. By utilizing a modular, transportable design and placement within existing facilities, the system can be transferred to different sites for reuse. The initial unit has now been removed from the process building and is presently being reinstalled for use in baseline operations at ORNL.

  20. Feasibility study of utilization of coal mine refuse, Estill County, Kentucky. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1976-08-01

    Reported is a feasibility study of commercial utilization for Eastern KY coal mine refuse at the South-East Coal Co. Cleaning plant in Estill County, KY. Included are descriptions of the physical facilities, sampling and testing methodology, geotechnical properties analyses, typical coal analyses, mineralogical characterizations, slow and quick firing test results, general marketing review of products, and environmental aspects. A good potential for economic development in Estill County exists with the anticipated uses of coal mine refuse as a raw material for the production of construction materials, particularly sintered lightweight aggregate. The refuse responded to the sintering process and a high quality, lightweight product was produced. The aggregate performed well in structural concrete and bituminous surfacing mixes. Other potential uses would be for lightweight concrete masonry blocks and soilless growing media. Inherent characteristics of the sintered material would provide for highly skid-resistant road surfacing aggregate and highly insulative structural building products. Market research results point to definitely feasible markets in East and East-Central KY and to the need for extensive, intensive marketing programs for commercial success of the proposed products. (Portions of this document are not fully legible)

  1. Direct utilization of geothermal energy for Pagosa Springs, Colorado. Final report, June 1979-June 1984

    Energy Technology Data Exchange (ETDEWEB)

    Goering, S.W.; Garing, K.L.; Coury, G.

    1984-08-01

    The Pagosa Springs Geothermal District Heating System was conceptualized, designed, and constructed between 1979 to 1984 under the US Department of Energy Program Opportunity Notice (PON) program to demonstrate the feasibility for utilizing moderate temperature geothermal resources for direct-use applications. The Pagosa Springs system successfully provides space heating to public buildings, school facilities, residences, and commercial establishments at costs significantly lower than costs of available conventional fuels. The Pagosa Springs project encompassed a full range of technical, institutional, and economic activities. Geothermal reservoir evaluations and testing were performed, and two productive approx.140/sup 0/F geothermal supply wells were successfully drilled and completed. Transmission and distribution system design, construction, startup, and operation were achieved with minimum difficulty. The geothermal system operation during the first two heating seasons has been fully reliable and well respected in the community. The project has proven that low to moderate-temperature waters can effectively meet required heating loads, even for harsh winter-mountain environments. The principal difficulty encountered has been institutional in nature and centers on the obtaining of the geothermal production well permits and the adjudicated water rights necessary to supply the geothermal hot water fluids for the full operating life of the system. 28 figs., 15 tabs.

  2. Direct utilization of geothermal energy in western South Dakota agribusiness. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Howard, S.M.

    1983-09-01

    This project involved the direct utilization of geothermal energy for (1) space heating of farm and ranch buildings, (2) drying grain, and (3) providing warm stock water during the winter. The site for this demonstration project was the Diamond Ring Ranch north of Midland, South Dakota. Geothermal water flowing from an existing well into the Madison Aquifer was used to heat four homes, a shop, a hospital barn for cattle, and air for a barn and grain dryer. This site is centrally located in the western region of South Dakota where geothermal water is available from the Madison Aquifer. The first year of the project involved the design of the heating systems and its construction while the following years were for operation, testing, demonstrating, and monitoring the system. Required modifications and improvements were made during this period. Operating modifications and improvements were made during this period. Operating experience showed that such application of geothermal resources is feasible and can result in substantial fuel savings. Economic analyses under a variety of assumptions generally gave payback periods of less than ten years. Numerous technical recommendations are made. The most significant being the necessity of passive protection from freezing of remote geothermal systems subject to winter shut downs caused by power or equipment failure. The primary institutional recommendation is to incorporate a use for the geothermal water such as irrigation or stock watering into agribusiness-related geothermal development.

  3. Utilization of fuel cells to beneficially use coal mine methane. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Brown, J.T.; O`Brien, D.G.; Miller, A.R.; Atkins, R.; Sanders, M.

    1996-03-01

    DOE has been given the responsibility to encourage industry to recover and use methane that is currently being released to the atmosphere. At this time the only method being employed at the Left Fork Mine to remove methane is the mine ventilation system. The methane content was measured at one one-hundredth of a percent. To prevent this methane from being vented to the atmosphere, degasification wells are proposed. To use the coal mine methane, it is proposed to use phosphoric-acid fuel cells to convert methane to electric power. These fuel cells contain (1) a steam reformer to convert the methane to hydrogen (and carbon dioxide), (2) the fuel cell stack, and (3) a power conditioner that provides 200 kW of 60 Hz alternating current output. The environmental impacts and benefits of using this technology ware summarized in the report. The study indicates the methane emission reduction that could be achieved on a national and Global level. The important point being that this technology is economically viable as is demonstrated in the report.

  4. Utilization of logging waste from mechanical spruce dominated final cuttings; Koneellisen puunkorjuun hakkuutaehteiden hyoedyntaeminen biopolttoaineena

    Energy Technology Data Exchange (ETDEWEB)

    Ebeling, J [Jaakko Poeyry Consulting Oy, Vantaa (Finland)

    1997-12-01

    The aim of the project has been to improve the economy of collecting logging waste from spruce dominated mechanised final felling. This section of the biomass is regarded as the most promising alternative biofuel source. The project compared different systems of collecting this raw material and concluded, that the most economical way to do this was (1) to integrate the transport of logging waste from the forest to the road side with the transport of logs using the equipment already at the site. The use of a separate tractor proved uneconomical compared to the integrated system. (2) Chip the logging waste at the road side with an integrated chipping and transport lorry (truck) equipped with three 20 feet standard or modified containers. The total cargo space in the lorry is thus around 100 m{sup 3} loose volume. The economical transport distance of this equipment is around under 100 km one way distance. The report contains also detailed drawings of the technical solution arrived at. The main idea is to use a module structure, where the chipper - the Bruks 803CT - is located together with most of the hydraulics, crane and the control equipment. The only outside connections needed are the hydraulic pressure from the pump and the operational unit with the necessary electrical panel. Thus the assembly and installation of the module on the lorry is rapid and the quality of the work can be maintained high. The operation is designed on the basis of one man operation and in such away that the need to for the driver-operator to step down from the controls is minimised. In normal situation the operation can be fully accomplished from the drivers cab - even when changing the containers

  5. Biochemical and physiological bases for utilization of dietary amino acids by young Pigs.

    Science.gov (United States)

    Rezaei, Reza; Wang, Weiwei; Wu, Zhenlong; Dai, Zhaolai; Wang, Junjun; Wu, Guoyao

    2013-02-27

    Protein is quantitatively the most expensive nutrient in swine diets. Hence it is imperative to understand the physiological roles played by amino acids in growth, development, lactation, reproduction, and health of pigs to improve their protein nutrition and reduce the costs of pork production. Due to incomplete knowledge of amino acid biochemistry and nutrition, it was traditionally assumed that neonatal, post-weaning, growing-finishing, and gestating pigs could synthesize sufficient amounts of all "nutritionally nonessential amino acids" (NEAA) to support maximum production performance. Therefore, over the past 50 years, much emphasis has been placed on dietary requirements of nutritionally essential amino acids as building blocks for tissue proteins. However, a large body of literature shows that NEAA, particularly glutamine, glutamate, arginine and proline regulate physiological functions via cell signaling pathways, such as mammalian target of rapamycin, AMP-activated protein kinase, extracellular signal-related kinase, Jun kinase, mitogen-activated protein kinase, and NEAA-derived gaseous molecules (e.g., nitric oxide, carbon monoxide, and hydrogen sulfide). Available evidence shows that under current feeding programs, only 70% and 55% of dietary amino acids are deposited as tissue proteins in 14-day-old sow-reared piglets and in 30-day-old pigs weaned at 21 days of age, respectively. Therefore, there is an urgent need to understand the roles and dietary requirements of NEAA in swine nutrition. This review highlights the basic biochemistry and physiology of absorption and utilization of amino acids in young pigs to enhance the efficacy of utilization of dietary protein and to minimize excretion of nitrogenous wastes from the body.

  6. Engineering Escherichia coli for biodiesel production utilizing a bacterial fatty acid methyltransferase.

    Science.gov (United States)

    Nawabi, Parwez; Bauer, Stefan; Kyrpides, Nikos; Lykidis, Athanasios

    2011-11-01

    The production of low-cost biofuels in engineered microorganisms is of great interest due to the continual increase in the world's energy demands. Biodiesel is a renewable fuel that can potentially be produced in microbes cost-effectively. Fatty acid methyl esters (FAMEs) are a common component of biodiesel and can be synthesized from either triacylglycerol or free fatty acids (FFAs). Here we report the identification of a novel bacterial fatty acid methyltransferase (FAMT) that catalyzes the formation of FAMEs and 3-hydroxyl fatty acid methyl esters (3-OH-FAMEs) from the respective free acids and S-adenosylmethionine (AdoMet). FAMT exhibits a higher specificity toward 3-hydroxy free fatty acids (3-OH-FFAs) than FFAs, synthesizing 3-hydroxy fatty acid methyl esters (3-OH-FAMEs) in vivo. We have also identified bacterial members of the fatty acyl-acyl carrier protein (ACP) thioesterase (FAT) enzyme family with distinct acyl chain specificities. These bacterial FATs exhibit increased specificity toward 3-hydroxyacyl-ACP, generating 3-OH-FFAs, which can subsequently be utilized by FAMTs to produce 3-OH-FAMEs. PhaG (3-hydroxyacyl ACP:coenzyme A [CoA] transacylase) constitutes an alternative route to 3-OH-FFA synthesis; the coexpression of PhaG with FAMT led to the highest level of accumulation of 3-OH-FAMEs and FAMEs. The availability of AdoMet, the second substrate for FAMT, is an important factor regulating the amount of methyl esters produced by bacterial cells. Our results indicate that the deletion of the global methionine regulator metJ and the overexpression of methionine adenosyltransferase result in increased methyl ester synthesis.

  7. Analysis of utility acid rain compliance plans: A discussion of issues and methods

    Energy Technology Data Exchange (ETDEWEB)

    Brick, S. [MSB Energy Associates, Inc., Middleton, WI (United States)

    1993-07-01

    Utility acid rain compliance plans have begun to roll into public utility commissions around the eastern half of the country. These plans typically represent the combined effort of utility staff and consultants, and have evolved since early drafts of Title IV of the Clean Air Act Amendments of 1990 (CAAA) began to circulate, several years back. The filings themselves often consist of many volumes of technical analysis, supporting documentation, and accompanying testimony. Hundreds of computer simulations are often presented and these, in turn, are underlain by hundreds of assumptions covering a staggering range of variables. Commissions usually have little time and few external resources with which to review and in some cases, preapprove proposed utility actions. There may be a tendency among commissions to forego a comprehensive review, approve utility plans as filed, and hope for the best. There are at least three important reasons, however, why this should not occur: (1) The magnitude of planned CAAA expenditures is large. (2) Preapproval, either formal or de facto, is becoming increasingly common. Although the approval of specific actions is certainly one outcome of the preapproval process, it should not be the sole or even principal outcome. The main focus of preapproval should involve defining an economic and institutional framework for how utilities, with input from regulators and the public, can best meet future customer needs. Done correctly, the preapproval process provides a meaningful, preexisting context which can be used to analyze outcomes. (3) Without adequate review market opportunities may be ignored and compliance costs increased unnecessarily. This article discusses the following topics in detail: CAAA Compliance Plan Review framework; Emission Reduction Alternatives: How Much is Enough?; Assumptions, Analytical Methods, and Evaluation Criteria of compliance plans; CAAA compliance costs and IRP. 2 figs., 3 tabs.

  8. Simulation of a Wet Sulfuric Acid Process (WSA for Utilization of Acid Gas Separated from Omani Natural Gas

    Directory of Open Access Journals (Sweden)

    Ahmed Jawad Ali Al-Dallal

    2013-06-01

    Full Text Available In this study, a proposed process for the utilization of hydrogen sulphide separated with other gases from omani natural gas for the production of sulphuric acid by wet sulphuric acid process (WSA was studied. The processwas simulated at an acid gas feed flow of 5000 m3/hr using Aspen ONE- V7.1-HYSYS software. A sensitivity analysis was conducted to determine the optimum conditions for the operation of plant. This included primarily the threepacked bed reactors connected in series for the production of sulphur trioxidewhich represented the bottleneck of the process. The optimum feed temperature and catalyst bed volume for each reactor were estimated and then used in the simulation of the whole process for two cases namely 4 and 6 mole% SO2 stream fed to the first catalytic reactor. The 4mole% SO2 gaves the highest conversion (98% compared with 6 mole% SO2 (94.7%. A valuable quantity of heat was generated from the process. This excess heat could also be transformed into power in a turbine or used as a heating media in neighbouring process units.

  9. Characterization of purple acid phosphatases involved in extracellular dNTP utilization in Stylosanthes.

    Science.gov (United States)

    Liu, Pan-Dao; Xue, Ying-Bin; Chen, Zhi-Jian; Liu, Guo-Dao; Tian, Jiang

    2016-07-01

    Stylo (Stylosanthes spp.) is a pasture legume predominant in tropical and subtropical areas, where low phosphorus (P) availability is a major constraint for plant growth. Therefore, stylo might exhibit superior utilization of the P pool on acid soils, particularly organic P. However, little is known about mechanisms of inorganic phosphate (Pi) acquisition employed by stylo. In this study, the utilization of extracellular deoxy-ribonucleotide triphosphate (dNTP) and the underlying physiological and molecular mechanisms were examined for two stylo genotypes with contrasting P efficiency. Results showed that the P-efficient genotype, TPRC2001-1, was superior to the P-inefficient genotype, Fine-stem, when using dNTP as the sole P source. This was reflected by a higher dry weight and total P content for TPRC2001-1 than for Fine-stem, which was correlated with higher root-associated acid phosphatase (APase) activities in TPRC2001-1 under low P conditions. Subsequently, three PAP members were cloned from TPRC2001-1: SgPAP7, SgPAP10, and SgPAP26 Expression levels of these three SgPAPs were up-regulated by Pi starvation in stylo roots. Furthermore, there was a higher abundance of transcripts of SgPAP7 and SgPAP10 in TPRC2001-1 than in Fine-stem. Subcellular localization analysis demonstrated that these three SgPAPs were localized on the plasma membrane. Overexpression of these three SgPAPs could result in significantly increased root-associated APase activities, and thus extracellular dNTP utilization in bean hairy roots. Taken together, the results herein suggest that SgPAP7, SgPAP10, and SgPAP26 may differentially contribute to root-associated APase activities, and thus control extracellular dNTP utilization in stylo.

  10. Diel Production and Microheterotrophic Utilization of Dissolved Free Amino Acids in Waters Off Southern California

    OpenAIRE

    Carlucci, A. F.; Craven, D. B.; Henrichs, S. M.

    1984-01-01

    Diel patterns of dissolved free amino acid (DFAA) concentration and microheterotrophic utilization were examined in the spring and fall of 1981 in euphotic waters from the base of the mixed layer off the southern California coast. The average depths of the isotherms sampled were 19.2 m for spring and 9.0 m for fall. Total DFAA levels were generally higher in the spring than in the fall, 18 to 66 nM and 14 to 20 nM, respectively. Two daily concentration maxima and minima were observed for tota...

  11. Mixed strategies for energy conservation and alternative energy utilization (solar) in buildings. Final report. Volume III. Appendixes. [10 appendices

    Energy Technology Data Exchange (ETDEWEB)

    None

    1977-06-01

    This appendix summarizes building characteristics used to determine heating and cooling loads for each of the five building types in each of the four regions. For the selected five buildings, the following data are attached: new and existing construction characteristics; new and existing construction thermal resistance; floor plan and elevation; people load schedule; lighting load schedule; appliance load schedule; ventilation schedule; and hot water use schedule. For the five building types (single family, apartment buildings, commercial buildings, office buildings, and schools), data are compiled in 10 appendices. These are Building Characteristics; Alternate Energy Sources and Energy Conservation Techniques Description, Costs, Fuel Price Scenarios; Life Cycle Cost Model; Simulation Models; Solar Heating/Cooling System; Condensed Weather; Single and Multi-Family Dwelling Characteristics and Energy Conservation Techniques; Mixed Strategies for Energy Conservation and Alternative Energy Utilization in Buildings. An extensive bibliography is given in the final appendix. (MCW)

  12. Nanoscopic imaging of chromatin topology utilizing intrinsic fluorescence from unmodified nucleic acids (Conference Presentation)

    Science.gov (United States)

    Dong, Biqin; Almassalha, Luay Matthew; Stypula-Cyrus, Yolanda; Urban, Ben E.; Chandler, John E.; Nguyen, The-Quyen; Sun, Cheng; Zhang, Hao F.; Backman, Vadim

    2017-02-01

    Imaging the nanoscale intracellular structures formed by nucleic acids, such as chromatin, in non-perturbed, structurally and dynamically complex cellular systems, will help improve our understanding of biological processes and open the next frontier for biological discovery. Current optical super-resolution fluorescence techniques require exogenous labels that may disrupt cell function and alter the subdiffractional macromolecular structures they are used to visualize. As a means for label-free optical super-resolution imaging, we examined the discovery of stochastic fluorescence switching of unmodified nucleic acids under visible light illumination. Utilizing this phenomenon and a single-molecule photon localization approach we generated subdiffraction-resolution images down to 20nm using intrinsic fluorescence from nucleic acids. Specifically, the nanoscale organization of interphase nuclei and mitotic chromosomes were imaged. Using such a method for visualization, we performed a quantitative analysis of the DNA occupancy level and a subdiffractional analysis of the chromosomal organization. These experiments demonstrate a new method for visualizing the nanoscopic features of macromolecular structures composed of nucleic acids without the need for exogenous labels.

  13. Synthesis of methyl-1-(tert-butoxycarbonylamino)-2-vinylcyclopropanecarboxylate via a Hofmann rearrangement utilizing trichloroisocyanuric acid as an oxidant.

    Science.gov (United States)

    Crane, Zackary D; Nichols, Paul J; Sammakia, Tarek; Stengel, Peter J

    2011-01-07

    A trichloroisocyanuric acid (TCCA) mediated Hofmann rearrangement was utilized to synthesize methyl-1-(tert-butoxycarbonylamino)-2-vinylcyclopropanecarboxylate. A variety of functional groups are tolerated in this reaction including vinyl, cyclopropyl, pyridyl, aryl, benzyl, and nitro groups.

  14. The effect of DL-2-bromopalmitate on the utilization of palmitic acid by rat granular pneumocytes.

    Science.gov (United States)

    Heinemann, H O; Pietra, G G; Wagner, M; Minda, M

    1979-02-06

    The effect of DL-2-bromopalmitate (BrPA), an analogue of palmitic acid (PA), on the utilization of this fatty acid by rat lungs was investigated by a combination of anatomic and biochemical methods. The experiments were performed in vitro on two types of preparations, isolated perfused lungs and lung slices. In the isolated lung preparation the substrate reached the lung via the capillaries, in lung slices via the alveolar epithelium. Electron microscope autoradiography showed that BrPA depressed uptake of PA by granular pneumocytes. Radioactivity recovered by tissue analysis and capture of CO2 established that PA oxidation and incorporation into phospholipids and triglycerides was depressed by BrPA. A close correlation was found between the reduction in radioactivity in phospholipids and the grain density over lamellar bodies. The study shows that BrPA reversibly interferes with the uptake and utilization of long chain fatty by granular pneumocytes. BrPA appears as a useful tool to study palmitate metabolism and surfactant production by the lung.

  15. Low-phytic acid barley improves calcium and phosphorus utilization and growth performance in growing pigs.

    Science.gov (United States)

    Veum, T L; Ledoux, D R; Bollinger, D W; Raboy, V; Cook, A

    2002-10-01

    Thirty-five crossbred barrows averaging 13.5 kg starting BW were used in a 35-d experiment to compare the availability of P and the nutritional value of two near-isogenic progeny of the barley cultivar 'Harrington'. Low-phytic acid barley (LPB, 0.35% total P, 0.14% phytic acid P) was homozygous for the low-phytic acid 1-1 allele, and the normal barley (NB, 0.35% total P, 0.24% phytic acid P) was homozygous for the normal allele of that gene. Pigs were fed individually twice daily in metabolism pens. Barley was the only source of phytate in semipurified diets, 1 to 3. Diet 1 contained 75% NB, 0.14% estimated available P (aP), and 0.50% Ca. Diet 2 contained 75% LPB, 0.22% aP, and 0.50% Ca. No inorganic P (iP) was added to Diets 1 and 2 in order to measure the animal response to the different concentrations of aP in these cultivars. Diet 3 was NB Diet 1 supplemented with iP to equal the concentration of aP in LPB Diet 2. Practical barley-soybean meal (SBM)-type diets, NB Diet 4 and LPB Diet 5, were formulated to meet all minimum nutrient requirements, and contained 0.30% aP and 0.65% Ca. For the semipurified diets, pigs fed LPB Diet 2 had higher (P or = 0.3) in growth performance, fresh bone weight, fat-free dry bone weight, bone ash, bone breaking strength, or N utilization. This indicates that LPB and NB were equal in nutritional value after supplementation of NB with iP to equal the estimated aP in LPB. For the practical barley-SBM diets, there were no differences (P > or = 0.4) between pigs fed NB Diet 4 or LPB Diet 5 for growth performance, fresh bone weight, bone breaking strength, the percentages of P and Ca utilization, or N, DE, and ME utilization. The use of LPB in pig diets reduced P excretion in swine waste by 55% and 16% in our semipurified and practical diets, respectively, compared with NB. Using our in vitro procedure designed to mimic the digestive system of the pig, the availability of P for pigs was estimated at 52% for LPB and 32% for NB.

  16. IDH1 deficiency attenuates gluconeogenesis in mouse liver by impairing amino acid utilization

    Science.gov (United States)

    Ye, Jing; Gu, Yu; Zhang, Feng; Zhao, Yuanlin; Yuan, Yuan; Hao, Zhenyue; Sheng, Yi; Li, Wanda Y.; Wakeham, Andrew; Cairns, Rob A.; Mak, Tak W.

    2017-01-01

    Although the enzymatic activity of isocitrate dehydrogenase 1 (IDH1) was defined decades ago, its functions in vivo are not yet fully understood. Cytosolic IDH1 converts isocitrate to α-ketoglutarate (α-KG), a key metabolite regulating nitrogen homeostasis in catabolic pathways. It was thought that IDH1 might enhance lipid biosynthesis in liver or adipose tissue by generating NADPH, but we show here that lipid contents are relatively unchanged in both IDH1-null mouse liver and IDH1-deficient HepG2 cells generated using the CRISPR-Cas9 system. Instead, we found that IDH1 is critical for liver amino acid (AA) utilization. Body weights of IDH1-null mice fed a high-protein diet (HPD) were abnormally low. After prolonged fasting, IDH1-null mice exhibited decreased blood glucose but elevated blood alanine and glycine compared with wild-type (WT) controls. Similarly, in IDH1-deficient HepG2 cells, glucose consumption was increased, but alanine utilization and levels of intracellular α-KG and glutamate were reduced. In IDH1-deficient primary hepatocytes, gluconeogenesis as well as production of ammonia and urea were decreased. In IDH1-deficient whole livers, expression levels of genes involved in AA metabolism were reduced, whereas those involved in gluconeogenesis were up-regulated. Thus, IDH1 is critical for AA utilization in vivo and its deficiency attenuates gluconeogenesis primarily by impairing α-KG–dependent transamination of glucogenic AAs such as alanine. PMID:28011762

  17. Time-Resolved Nucleic Acid Hybridization Beacons Utilizing Unimolecular and Toehold-Mediated Strand Displacement Designs.

    Science.gov (United States)

    Massey, Melissa; Ancona, Mario G; Medintz, Igor L; Algar, W Russ

    2015-12-01

    Nucleic acid hybridization probes are sought after for numerous assay and imaging applications. These probes are often limited by the properties of fluorescent dyes, prompting the development of new probes where dyes are paired with novel or nontraditional luminescent materials. Luminescent terbium complexes are an example of such a material, and these complexes offer several unique spectroscopic advantages. Here, we demonstrate two nonstem-loop designs for light-up nucleic acid hybridization beacons that utilize time-resolved Förster resonance energy transfer (TR-FRET) between a luminescent Lumi4-Tb cryptate (Tb) donor and a fluorescent reporter dye, where time-resolved emission from the dye provides an analytical signal. Both designs are based on probe oligonucleotides that are labeled at their opposite termini with Tb and a fluorescent reporter dye. In one design, a probe is partially blocked with a quencher dye-labeled oligonucleotide, and target hybridization is signaled through toehold-mediated strand displacement and loss of a competitive FRET pathway. In the other design, the intrinsic folding properties of an unblocked probe are utilized in combination with a temporal mechanism for signaling target hybridization. This temporal mechanism is based on a recently elucidated "sweet spot" for TR-FRET measurements and exploits distance control over FRET efficiencies to shift the Tb lifetime within or outside the time-gated detection window for measurements. Both the blocked and unblocked beacons offer nanomolar (femtomole) detection limits, response times on the order of minutes, multiplexing through the use of different reporter dyes, and detection in complex matrices such as serum and blood. The blocked beacons offer better mismatch selectivity, whereas the unblocked beacons are simpler in design. The temporal mechanism of signaling utilized with the unblocked beacons also plays a significant role with the blocked beacons and represents a new and effective

  18. Evaluation of phytic acid utilization by S. cerevisiae strains used in fermentation processes and biomass production.

    Science.gov (United States)

    Mikulski, Dawid; Kłosowski, Grzegorz

    2017-01-01

    Saccharomyces cerevisiae is a well-studied yeast species used mainly in fermentation processes, bakery, and for SCP (Single Cell Protein) acquisition. The aim of the study was to analyze the possibility of phytic acid utilization as one of the hydrolysis processes carried out by yeast. The analysis of 30 yeast strains used in fermentation and for biomass production, that were grown in media containing phytic acid, revealed a high variability in the biomass production rate and the capability to hydrolyze phytates. No correlation between a high biomass concentration and a high level of phytate hydrolysis was found. Only four analyzed strains (Bayanus IOC Efficience, Sano, PINK EXCEL, FINAROME) were able to reduce the phytic acid concentration by more than 33.5%, from the initial concentration 103.0 ± 2.1 μg/ml to the level below 70 μg/ml. The presented results suggest that the selected wine and fodder yeast can be used as in situ source of phosphohydrolases in fermentation processes, and especially in the production of fodder proteins. However, further studies aimed at the optimization of growing parameters, such as the maximization of phytase secretion, and a comprehensive analysis of the catalytic activity of the isolated phosphohydrolases, are necessary.

  19. Utilization of Shochu distillery wastewater for production of polyunsaturated fatty acids and xanthophylls using thraustochytrid.

    Science.gov (United States)

    Yamasaki, Takashi; Aki, Tsunehiro; Shinozaki, Masami; Taguchi, Masahiro; Kawamoto, Seiji; Ono, Kazuhisa

    2006-10-01

    The industrial production of barley shochu, a distilled alcoholic beverage, results in distillery waste that is currently incinerated or disposed of in landfills, causing environmental pollution. The supernatant of distillery waste contains organic matter such as proteins ( approximately 2.5%) and amino acids ( approximately 0.2%). This study demonstrates that the utilization of distillery wastewater as a sole nitrogen source enables a marine thraustochytrid, Schizochytrium sp. strain KH105, to propagate and accumulate valuable lipids including docosahexaenoic acid (DHA) and astaxanthin. Under optimized culture conditions, the highest DHA and astaxanthin yields were obtained at 3.4 g/l and 7.7 mg/l, respectively, after 4 or 5 d of cultivation in a 3-l jar fermentor. The chemical oxygen demand of the wastewater was reduced by 35%. About 67% of crude protein content and 85% of total free amino acid content also decreased in the culture supernatant. The thraustochytrid therefore serves to upgrade the distillery by-product to one containing value-added lipids for functional foods as well as to regulate the environmental contamination.

  20. PPARgamma agonist induced cardiac enlargement is associated with reduced fatty acid and increased glucose utilization in myocardium of Wistar rats.

    Science.gov (United States)

    Edgley, Amanda J; Thalén, Pia G; Dahllöf, Björn; Lanne, Boel; Ljung, Bengt; Oakes, Nicholas D

    2006-05-24

    In toxicological studies, high doses of peroxisome proliferator-activated receptor-gamma (PPARgamma) agonists cause cardiac enlargement. To investigate whether this could be explained by a large shift from free fatty acid to glucose utilization by the heart, Wistar rats were treated for 2-3 weeks with a potent, selective PPARgamma agonist (X334, 3 micromol/kg/d), or vehicle. X334 treatment increased body-weight gain and ventricular mass. Treatment lowered plasma triglycerides by 61%, free fatty acid levels by 72%, insulin levels by 45%, and reduced total plasma protein concentration by 7% (indicating plasma volume expansion) compared to vehicle animals. Fasting plasma glucose levels were unaltered. To assess cardiac free fatty acid and glucose utilization in vivo we used simultaneous infusions of non-beta-oxidizable free fatty acid analogue, [9,10-(3)H](R)-2-bromopalmitate and [U-(14)C]2-deoxy-d-glucose tracers, which yield indices of local free fatty acid and glucose utilization. In anesthetized, 7 h fasted animals, left ventricular glucose utilization was increased to 182% while free fatty acid utilization was reduced by 28% (P<0.05) compared to vehicle. In separate studies we attempted to prevent the X334-induced hypolipidemia. Various dietary fat supplements were unsuccessful. By contrast, restricting the time during which the treated animals had access to food (promoting endogenous lipolysis), restored plasma free fatty acid from 27% to 72% of vehicle control levels and prevented the cardiac enlargement. Body-weight gain in these treated-food restricted rats was not different from vehicle controls. In conclusion, the cardiac enlargement caused by intense PPARgamma activation in normal animals is associated with marked changes in free fatty acid/glucose utilization and the enlargement can be prevented by restoring free fatty acid availability.

  1. Sorbent utilization studies using a mini-pilot spray dryer. Final report, 1 September 1992--31 August 1993

    Energy Technology Data Exchange (ETDEWEB)

    Keener, T.C.; Khang, S.J.; Wang, J.; Sanders, J.F.

    1993-09-30

    The main body of the report consists of four parts: 1. additives to change process chemistry for SO{sub 2} absorption by Ca(OH){sub 2} slurry; 2. recycle tests and hydration of fly ash with Ca(OH){sub 2} to increase reactivity; 3. limestone as an alternative sorbent and additive effects; 4. physical and chemical model developments for some of the additive effects and spray dryer mathematical model application. As the concentration of SO{sub 2} in the flue gases increases, the SO{sub 2} removal efficiency will go down. Additives such as delinquent salts (NaOH, NaCl, and NaHCO{sub 3}) have been shown to improve SO{sub 2} uptake, and these additive tests have indicated that SO{sub 2} uptake may be increased by as much as 60% over baseline conditions. Other additives such as H{sub 2}O{sub 2}, sugar, and some organic acids which can change the chemical reaction processes are suggested and tested, and some promising results have been obtained. Recycle has been shown to increase sorbent utilization by allowing partially reacted sorbent to react further with the SO{sub 2} in the flue gases. Two types of Ohio coal fly ashes have been extensively studied, and improvement of utilization in spray dryer flue gas desulfurization has been demonstrated. Limestone represents an area where significant cost savings can be realized. The spray dryer tests were designed to provide some results for understanding the magnitude of the limestone performance in the spray dryer system and the additive effects. The additive effects on increasing SO{sub 2} absorption by Ca(OH){sub 2} slurry were investigated, and the chemical and physical properties of these tested additives were studied. Some models have been formed to explain the additive phenomena.

  2. Uptake and/or utilization of two simple phenolic acids by Cucumis sativus

    Energy Technology Data Exchange (ETDEWEB)

    Shann, J.R.

    1986-01-01

    The uptake of ferulic acid (FA) and p-hydroxybenzoic acid (p-HBA) from solutions (0.1 to 1.00 mM, pH 4.0 to 7.0), was determined for intact and excised roots of Cucumis sativus. Uptake methods based on high performance liquid chromatographic (HPLC) analysis of phenolic acid depletion from solution were compared to those radioisotopic methods employing (U-ring-/sup 14/C)FA or p-HBA. Although radiotracer methods more accurately reflected actual uptake of the compounds by cucumber seedlings, HPLC solution depletion methods may be useful in the elucidation of trends over very limited periods of time. The uptake of FA was unaffected by the presence of p-HBA. The uptake of p-HBA was reduced by 30% in the presence of FA when compared to the uptake from solutions containing p-HBA alone. Ferulic acid acts both as an allelopathic agent and precursor in the endogenous process of lignification. To evaluate the involvement of exogenous FA in lignin biosynthesis, roots of hydroponically grown cucumber seedlings were exposed to concentrations of FA labeled with (U-ring-/sup 14/C)FA. Radiotracer was distributed throughout the seedling. A quantitative change in lignification occurred in treated seedlings. In roots and stems, the level of lignin increased with the number of exposures and as the concentrations of exogenous FA increased. Radiotracer was found in the residues of lignin isolated from seedling tissue treated with (U-ring-/sup 14/C)FA. This suggested the utilization of the exogenously applied FA in the endogenous process of lignification.

  3. C- and N-catabolic utilization of tricarboxylic acid cycle-related amino acids by Scheffersomyces stipitis and other yeasts.

    Science.gov (United States)

    Freese, Stefan; Vogts, Tanja; Speer, Falk; Schäfer, Bernd; Passoth, Volkmar; Klinner, Ulrich

    2011-05-01

    Scheffersomyces stipitis and the closely related yeast Candida shehatae assimilated the L-amino acids glutamate, aspartate and proline as both carbon and nitrogen sole sources. We also found this rarely investigated ability in ascomycetous species such as Candida glabrata, C. reukaufii, C. utilis, Debaryomyces hansenii, Kluyveromyces lactis, K. marxianus, Candida albicans, L. elongisporus, Meyerozyma guilliermondii, C. maltosa, Pichia capsulata and Yarrowia lipolytica and in basidiomycetous species such as Rhodotorula rubra and Trichosporon beigelii. Glutamate was a very efficient carbon source for Sc. stipitis, which enabled a high biomass yield/mole, although the growth rate was lower when compared to growth on glucose medium. The cells secreted waste ammonium during growth on glutamate alone. In Sc. stipitis cultures grown in glucose medium containing glutamate as the nitrogen source the biomass yield was maximal, and ethanol concentration and specific ethanol formation rate were significantly higher than in glucose medium containing ammonium as the nitrogen source. Mainly C-assimilation of glutamate but also N-assimilation in glucose-containing medium correlated with enhanced activity of the NAD-dependent glutamate dehydrogenase 2 (GDH2). A Δgdh2 disruptant was unable to utilize glutamate as either a carbon or a nitrogen source; moreover, this disruptant was also unable to utilize aspartate as a carbon source. The mutation was complemented by retransformation of the GDH2 ORF into the Δgdh2 strain. The results show that Gdh2p plays a dual role in Sc. stipitis as both C- and N-catabolic enzyme, which indicates its role as an interface between the carbon and nitrogen metabolism of this yeast.

  4. Copper catalysis for enhancement of cobalt leaching and acid utilization efficiency in microbial fuel cells.

    Science.gov (United States)

    Liu, Yaxuan; Shen, Jingya; Huang, Liping; Wu, Dan

    2013-11-15

    Enhancement of both cobalt leaching from LiCoO2 and acid utilization efficiency (AUE) in microbial fuel cells (MFCs) was successfully achieved by the addition of Cu(II). A dosage of 10mg/L Cu(II) improved both cobalt leaching up to 308% and AUE of 171% compared to the controls with no presence of Cu(II). The apparent activation energy of cobalt leaching catalyzed by Cu(II) in MFCs was only 11.8 kJ/mol. These results demonstrate cobalt leaching in MFCs using Cu(II) as a catalyst may be an effective strategy for cobalt recovery and recycle of spent Li-ion batteries, and the evidence of influence factors including solid/liquid ratio, temperature, and pH and solution conductivity can contribute to improving understanding of and optimizing cobalt leaching catalyzed by Cu(II) in MFCs.

  5. Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus

    Directory of Open Access Journals (Sweden)

    A. Baptista-Neto

    2000-12-01

    Full Text Available The kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium, glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, Y X/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide.

  6. Advanced Acid Gas Separation Technology for the Utilization of Low Rank Coals

    Energy Technology Data Exchange (ETDEWEB)

    Kloosterman, Jeff

    2012-12-31

    Air Products has developed a potentially ground-breaking technology – Sour Pressure Swing Adsorption (PSA) – to replace the solvent-based acid gas removal (AGR) systems currently employed to separate sulfur containing species, along with CO{sub 2} and other impurities, from gasifier syngas streams. The Sour PSA technology is based on adsorption processes that utilize pressure swing or temperature swing regeneration methods. Sour PSA technology has already been shown with higher rank coals to provide a significant reduction in the cost of CO{sub 2} capture for power generation, which should translate to a reduction in cost of electricity (COE), compared to baseline CO{sub 2} capture plant design. The objective of this project is to test the performance and capability of the adsorbents in handling tar and other impurities using a gaseous mixture generated from the gasification of lower rank, lignite coal. The results of this testing are used to generate a high-level pilot process design, and to prepare a techno-economic assessment evaluating the applicability of the technology to plants utilizing these coals.

  7. Influence of protein source on amino acid uptake patterns and protein utilization in rainbow trout Oncorhynchus mykiss

    DEFF Research Database (Denmark)

    Rolland, Marine; Holm, Jørgen; Dalsgaard, Anne Johanne Tang

    acids to the fishmeal diet level (see Table 1). Amino acid uptake patterns were assessed by the appearance of amino acids in the blood stream following the ingestion of a meal, while dietary protein utilization was evaluated by examining the metabolic response to digestion and ammonium and urea...... described time sampling. Fish were killed by a blow in the head and blood was collected from the caudal vein with heparinized syringes. Plasma and red blood cells content were stored separately at -80 for amino acid content analysis. The ammonium excretion profiles (Figure 1) will be correlated...... with the amino acid profile in the blood and oxygen consumption during digestion to investigate the effect on protein utilization for each treatment...

  8. A facile nanoaggregation strategy for oral delivery of hydrophobic drugs by utilizing acid base neutralization reactions

    Science.gov (United States)

    Chen, Huabing; Wan, Jiangling; Wang, Yirui; Mou, Dongsheng; Liu, Hongbin; Xu, Huibi; Yang, Xiangliang

    2008-09-01

    Nanonization strategies have been used to enhance the oral availability of numerous drugs that are poorly soluble in water. Exploring a facile nanonization strategy with highly practical potential is an attractive focus. Here, we report a novel facile nanoaggregation strategy for constructing drug nanoparticles of poorly soluble drugs with pH-dependent solubility by utilizing acid-base neutralization in aqueous solution, thus facilitating the exploration of nanonization in oral delivery for general applicability. We demonstrate that hydrophobic itraconazole dissolved in acid solution formed a growing core and aggregated into nanoparticles in the presence of stabilizers. The nanoparticles, with an average diameter of 279.3 nm and polydispersity index of 0.116, showed a higher dissolution rate when compared with the marketed formulation; the average dissolution was about 91.3%. The in vivo pharmacokinetic studies revealed that the nanoparticles had a rapid absorption and enhanced oral availability. The diet state also showed insignificant impact on the absorption of itraconazole from nanoparticles. This nanoaggregation strategy is a promising nanonization method with a facile process and avoidance of toxic organic solvents for oral delivery of poorly soluble drugs with pH-dependent solubility and reveals a highly practical potential in the pharmaceutical and chemical industries.

  9. Study on the spectrophotometric detection of free fatty acids in palm oil utilizing enzymatic reactions.

    Science.gov (United States)

    Azeman, Nur Hidayah; Yusof, Nor Azah; Abdullah, Jaafar; Yunus, Robiah; Hamidon, Mohd Nizar; Hajian, Reza

    2015-07-07

    In this paper, a comprehensive study has been made on the detection of free fatty acids (FFAs) in palm oil via an optical technique based on enzymatic aminolysis reactions. FFAs in crude palm oil (CPO) were converted into fatty hydroxamic acids (FHAs) in a biphasic lipid/aqueous medium in the presence of immobilized lipase. The colored compound formed after complexation between FHA and vanadium (V) ion solution was proportional to the FFA content in the CPO samples and was analyzed using a spectrophotometric method. In order to develop a rapid detection system, the parameters involved in the aminolysis process were studied. The utilization of immobilized lipase as catalyst during the aminolysis process offers simplicity in the product isolation and the possibility of conducting the process under extreme reaction conditions. A good agreement was found between the developed method using immobilized Thermomyces lanuginose lipase as catalyst for the aminolysis process and the Malaysian Palm Oil Board (MPOB) standard titration method (R2 = 0.9453).

  10. Clinical utility of 5-aminolevulinic acid HCl to better visualize and more completely remove gliomas

    Directory of Open Access Journals (Sweden)

    Halani SH

    2016-09-01

    Full Text Available Sameer H Halani,1 D Cory Adamson1,2 1Department of Neurosurgery, Emory University School of Medicine, Atlanta, GA, USA; 2Neurosurgery Section, Atlanta VA Medical Center, Decatur, GA, USA Abstract: Surgical resection is typically the first line of treatment for gliomas. However, the neurosurgeon faces a major challenge in achieving maximal resection in high-grade gliomas as these infiltrative tumors make it difficult to discern tumor margins from normal brain with conventional white-light microscopy alone. To aid in resection of these infiltrative tumors, fluorescence-guided surgery has gained much popularity in intraoperative visualization of malignant gliomas, with 5-aminolevulinic acid (5-ALA leading the way. First introduced in an article in Neurosurgery, 5-ALA has since become a safe, effective, and inexpensive method to visualize and improve resection of gliomas. This has undoubtedly led to improvements in the clinical course of patients as demonstrated by the increased overall and progression-free survival in patients with such devastating disease. This literature review aims to discuss the major studies and trials demonstrating the clinical utility of 5-ALA and its ability to aid in complete resection of malignant gliomas. Keywords: aminolevulinic acid, 5-ALA, fluorescence, glioblastoma multiforme, high-grade glioma, resection

  11. Controls on the microbial utilization of carbon monoxide and formic acid in Acidic Hydrothermal Springs in Yellowstone National Park

    Science.gov (United States)

    Urschel, M.; Kubo, M. W.; Hoehler, T. M.; Boyd, E. S.; Peters, J.

    2012-12-01

    In hydrothermal systems, dissolved carbon dioxide (CO2) in the presence of reduced iron-bearing minerals, such as those found in basalt, can be reduced to form formic acid (HCOOH). HCOOH can then be dehydrated in a side reaction, resulting in the generation of carbon monoxide (CO), which forms an equilibrium with HCOOH. HCOOH can also be further reduced to methane, and longer chain hydrocarbons. Geochemical measurements have demonstrated the presence of elevated concentrations of HCOOH, dissolved CO, and dissolved inorganic carbon (CO2, H2CO3), in high temperature, low pH springs in Yellowstone National Park (YNP). Likewise, a number of compounds that could potentially serve as electron acceptors (e.g. S0, SO42-, NO3-, Fe3+) in the oxidation of CO or formic acid have been detected in many of these systems. We hypothesized that the utilization of CO and HCOOH as carbon and/or energy sources is a broadly-distributed metabolic strategy in high temperature, low pH springs in YNP. To test this hypothesis, radiolabeled CO (14CO) and HCOOH (H14COOH) were used to determine rates of CO and formate oxidation activity in three hot springs in YNP ranging in temperature from 53 °C to 89 °C and pH from 2.5 to 5.3. In parallel, 16S rRNA gene sequencing and enrichment isolation techniques were employed to identify the microorganisms responsible for these activities. Our results indicate that CO and HCOOH are important sources of carbon and/or energy in high temperature, low pH hydrothermal springs in Yellowstone National Park. Rates of CO oxidation appear to be orders of magnitude lower than those of HCOOH oxidation. One possible explanation for this result is that HCOOH is preferentially utilized, consistent with thermodynamic calculations indicating that HCOOH liberates approximately 215 kJ/mol more Gibbs energy (under standard conditions) than CO when oxidized with oxygen (O2) as the electron acceptor. Redox couples of HCOOH oxidation with other electron acceptors (e.g. SO4

  12. Utilization of some non coded amino acids as isosters of peptide building blocks.

    Science.gov (United States)

    Hlaváček, J; Marcová, R; Ježek, R; Slaninová, J

    1996-09-01

    In our study on non coded amino acids and their utilization in peptide chemistry we synthesized methylene-thio (CH2-S) and methyleneoxy (CH2-O) group containing amino acids and pseudodipeptides which could be used as building blocks for the construction of peptide hormone analogues. The (CH2-S) isoster of peptide bond exhibits increased flexibility, lipophility and resistance to proteolytic enzymes. This group exhibits similar properties as the isosteric disulfide bond in the side chain of cystine residue. The (CH2-O) isoster is moreover similar in its geometry to extended conformation of peptide bond. As a consequence, the changed profile of biological activities could be expected for peptide hormone analogues containing such isosteric moiety. The (CH2-S) isosters of the peptide bond were prepared by alkylation of thiolates of 2-mercaptocarboxylic acids, the disulfide bond by alkylation of cysteine or homocysteine. The (CH2-O) isosters were prepared by (AcO)4Rh2 catalyzed addition of carbenes of alkyl diazocarboxylates to N-protected aminoalcohols. Pseudodipeptides H-Leu-ψ(CH2-S)-Gly-NH2 and H-Leu-ψ(CH2-O)-Gly-NH2 were introduced into the C-terminal part of the oxytocin molecule using solution methods of peptide chemistry. Both inserted isosteric bonds were resistant against proteolytic degradation, the first one was found to decrease an enzymic cleavage of the distant Tyr(2)-Ile(3) bond in the corresponding analogue, too. The (CH2-S) isosters of the disulfide bond containing an orthogonal protection of theirα-amino (Fmoc) andα-(OAll, OH) orω-(OBu(+), OH) carboxylic groups were applied in the solid phase synthesis of the aminoterminal 1-deamino-15-pentadecapeptide of endothelin-I which represents a strong vasoactive agent. The solid phase synthesis was carried out by the step-wise protocol on the Rink or Merrifield type resin using orthogonally protected carba cystine building blocks.

  13. Amino Acid and Peptide Utilization Profiles of the Fluoroacetate-Degrading Bacterium Synergistetes Strain MFA1 Under Varying Conditions.

    Science.gov (United States)

    Leong, Lex E X; Denman, Stuart E; Hugenholtz, Philip; McSweeney, Christopher S

    2016-02-01

    Synergistetes strain MFA1 is an asaccharolytic ruminal bacterium isolated based on its ability to degrade fluoroacetate, a plant toxin. The amino acid and peptide requirements of the bacterium were investigated under different culturing conditions. The growth of strain MFA1 and its fluoroacetate degradation rate were enhanced by peptide-rich protein hydrolysates (tryptone and yeast extract) compared to casamino acid, an amino acid-rich protein hydrolysate. Complete utilization and preference for arginine, asparagine, glutamate, glycine, and histidine as free amino acids from yeast extract were observed, while the utilization of serine, threonine, and lysine in free form and peptide-bound glutamate was stimulated during growth on fluoroacetate. A predominant peptide in yeast extract preferentially utilized by strain MFA1 was partially characterized by high-liquid performance chromatography-mass spectrometry as a hepta-glutamate oligopeptide. Similar utilization profiles of amino acids were observed between the co-culture of strain MFA1 with Methanobrevibacter smithii without fluoroacetate and pure strain MFA1 culture with fluoroacetate. This suggests that growth of strain MFA1 could be enhanced by a reduction of hydrogen partial pressure as a result of hydrogen removal by a methanogen or reduction of fluoroacetate.

  14. Regulatory role for L-arginine in the utilization of amino acids by pig small-intestinal bacteria.

    Science.gov (United States)

    Dai, Zhao-Lai; Li, Xi-Long; Xi, Peng-Bin; Zhang, Jing; Wu, Guoyao; Zhu, Wei-Yun

    2012-07-01

    We recently reported that bacteria from the pig small intestine rapidly utilize and metabolize amino acids (AA). This study investigated the effect of L-arginine on the utilization of AA by pure bacterial strains (Streptococcus sp., Escherichia coli and Klebsiella sp.) and mixed bacterial cultures derived from the pig small intestine. Bacteria were incubated at 37°C for 3 h in anaerobic AA media containing 0-5 mmol/L of arginine to determine the effect of arginine on the bacterial utilization of AA. Amino acids in the medium plus cell extracts were analyzed by high-performance liquid chromatography. Results indicated concentration-dependent increases in the bacterial utilization of arginine and altered fluxes of arginine into ornithine and citrulline in the bacteria. Net glutamine utilization increased in pure bacterial strains with increased concentrations of arginine. With the addition of arginine, net utilization of threonine, glycine, phenylalanine and branched-chain AA increased (P<0.05) in Streptococcus sp. and Klebsiella sp., but decreased in E. coli. Net utilization of lysine, threonine, isoleucine, leucine, glycine and alanine by jejunal or ileal mixed bacteria decreased (P<0.05) with the addition of arginine. Complete utilization of asparagine, aspartate and serine were observed in pig small-intestinal bacteria after 3 h of incubation. Overall, the addition of arginine affected the metabolism of the arginine-family of AA and the serine- and aspartate-family of AA in small-intestinal bacteria and reduced the utilization of most AA in ileal mixed bacteria. These novel findings indicate that arginine exerts its beneficial effects on swine nutrition partially by regulating AA utilization and metabolism in the small-intestinal microbiota.

  15. Utilization of barley or wheat bran to bioconvert glutamate to γ-aminobutyric acid (GABA).

    Science.gov (United States)

    Jin, Wen-Jie; Kim, Min-Ju; Kim, Keun-Sung

    2013-09-01

    This study deals with the utilization of agro-industrial wastes created by barley and wheat bran in the production of a value-added product, γ-aminobutyric acid (GABA). The simple and eco-friendly reaction requires no pretreatment or microbial fermentation steps but uses barley or wheat bran as an enzyme source, glutamate as a substrate, and pyridoxal 5'-phosphate (PLP) as a cofactor. The optimal reaction conditions were determined on the basis of the temperatures and times used for the decarboxylation reactions and the initial concentrations of barley or wheat bran, glutamate, and PLP. The optimal reactions produced 9.2 mM of GABA from 10 mM glutamate, yielding a 92% GABA conversion rate, when barley bran was used and 6.0 mM of GABA from 10 mM glutamate, yielding a 60% GABA conversion rate, when wheat bran was used. The results imply that barley bran is more efficient than wheat bran in the production of GABA.

  16. Absorption and utilization of organic matter by the strict autotroph, Thiobacillus thiooxidans, with special reference to aspartic acid.

    Science.gov (United States)

    Butler, R G; Umbreit, W W

    1966-02-01

    Butler, Richard G. (Rutgers, The State University, New Brunswick, N.J.), and Wayne W. Umbreit. Absorption and utilization of organic matter by the strict autotroph, Thiobacillus thiooxidans, with special reference to aspartic acid. J. Bacteriol. 91:661-666. 1966.-The strictly autotrophic bacterium, Thiobacillus thiooxidans, can be shown to assimilate a variety of organic materials. Aspartic acid can be assimilated into protein and can be converted into CO(2), but even in the presence of sulfur it cannot serve as the sole source of carbon for growth. The reason appears to be that aspartic acid is converted into inhibitory materials.

  17. Dietary Amino Acid Deficiency Reduces the Utilization of Amino Acids for Growth in Growing Pigs after a Period of Poor Health

    NARCIS (Netherlands)

    Hoek, van de E.; Jansman, A.J.M.; Borne, van den J.J.G.C.; Peet-Schwering, van der C.M.C.; Beers-Schreurs, van H.M.G.; Gerrits, W.J.J.

    2016-01-01

    Background: During immune system activation, partitioning of amino acids (AAs) changes between protein gain and use by the immune system. Objective: We determined the effects of health status and dietary AA deficiency on nitrogen retention and AA utilization in pigs. Methods: Barrows (55 d of age)

  18. Influence of selenomethionine and omega-3 fatty acid on serum mineral profile and nutrient utilization of broiler chicken

    Directory of Open Access Journals (Sweden)

    Pankaj Kumar

    2015-02-01

    Full Text Available Aim: This study was conducted to investigate the effect of selenomethionine and omega-3 fatty acid on serum mineral profile and nutrient utilization of broiler chicken. Materials and Methods: The present study was a 2×3 factorial arrangement of two levels of selenomethionine (0 and 0.3 ppm and three levels of omega-3 fatty acid (0, 0.5 and 1%. Day-old Vencobb broiler chicks (n=180, were randomly assigned in six treatment groups. The experiment lasted for 42 days. Treatment groups followed of: Group I was a control. Group II, III, IV, V and VI were supplemented with 0 ppm selenomethionine with 0.5% omega-3 fatty acid, 0 ppm selenomethionine with 1% omega-3 fatty acid, 0.3 ppm selenomethionine with 0% omega-3 fatty acid, 0.3 ppm selenomethionine with 0.5% omega-3 fatty acid and 0.3 ppm selenomethionine with 1% omega-3 fatty acid, respectively. Linseed oil was used as a source of omega-3 fatty acid while sel-plex is used for selenomethionine supplementation. Results: Significant (p<0.05 interaction exist between selenomethionine and omega-3 fatty acid for serum zinc and iron concentration whereas, it was non-significant for serum calcium and copper. Significantly (p<0.05 increased concentration of selenium, zinc, iron and phosphorus was observed in birds fed 0.3 ppm selenomethionine whereas, significantly (p<0.05 increased zinc and iron was observed in birds fed 0.5% omega-3 fatty acid. There was significant (p<0.05 interaction exist between selenomethionine and omega-3 fatty acid for calcium and phosphorus retention percentage. The maximum retention of calcium and phosphorus was recorded in birds supplemented with 0.3 ppm selenomethionine in combination with 0.5% omega-3 fatty acid. There was marked interaction between selenomethionine and omega-3 fatty acid for hemoglobin (Hb, total erythrocytic count, total leukocytic count and platelets (p<0.05 however, it was non-significant for mean corpuscular volume, mean corpuscular Hb, MCH concentration

  19. Final technical report: Commercialization of the Biofine technology for levulinic acid production from paper sludge

    Energy Technology Data Exchange (ETDEWEB)

    Fitzpatrick, Stephen W.

    2002-04-23

    This project involved a three-year program managed by BioMetics, Inc. (Waltham, MA) to demonstrate the commercial feasibility of Biofine thermochemical process technology for conversion of cellulose-containing wastes or renewable materials into levulinic acid, a versatile platform chemical. The program, commencing in October 1995, involved the design, procurement, construction and operation of a plant utilizing the Biofine process to convert 1 dry ton per day of paper sludge waste. The plant was successfully designed, constructed, and commissioned in 1997. It was operated for a period of one year on paper sludge from a variety of source paper mills to collect data to verify the design for a commercial scale plant. Operational results were obtained for four different feedstock varieties. Stable, continuous operation was achieved for two of the feedstocks. Continuous operation of the plant at demonstration scale provided the opportunity for process optimization, development of operational protocols, operator training and identification of suitable materials of construction for scale up to commercial operation . Separated fiber from municipal waster was also successfully processed. The project team consisted of BioMetics Inc., Great Lakes Chemical Corporation (West Lafayette, IN), and New York State Energy Research and Development Authority (Albany, NY).

  20. Field Operations Program Chevrolet S-10 (Lead-Acid) Accelerated Reliability Testing - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    J. Francfort (INEEL); J. Argueta; M. Wehrey (Southern California Edison); D. Karner; L. Tyree (Electric Transportation Applications)

    1999-07-01

    This report summarizes the Accelerated Reliability testing of five lead-acid battery-equipped Chevrolet S-10 electric vehicles by the US Department of Energy's Field Operations Program and the Program's testing partners, Electric Transportation Applications (ETA) and Southern California Edison (SCE). ETA and SCE operated the S-10s with the goal of placing 25,000 miles on each vehicle within 1 year, providing an accelerated life-cycle analysis. The testing was performed according to established and published test procedures. The S-10s' average ranges were highest during summer months; changes in ambient temperature from night to day and from season-to-season impacted range by as much as 10 miles. Drivers also noted that excessive use of power during acceleration also had a dramatic effect on vehicle range. The spirited performance of the S-10s created a great temptation to inexperienced electric vehicle drivers to ''have a good time'' and to fully utilize the S-10's acceleration capability. The price of injudicious use of power is greatly reduced range and a long-term reduction in battery life. The range using full-power accelerations followed by rapid deceleration in city driving has been 20 miles or less.

  1. Identification, definition and evaluation of potential impacts facing the US electric utility industry over the next decade. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Grainger, J.J.; Lee, S.S.H.

    1993-11-26

    There are numerous conditions of the generation system that may ultimately develop into system states affecting system reliability and security. Such generation system conditions should also be considered when evaluating the potential impacts on system operations. The following five issues have been identified to impact system reliability and security to the greatest extent: transmission access/retail wheeling; non-utility generators and independent power producers; integration of dispersed storage and generation into utility distribution systems; EMF and right-of-way limitations; Clean Air Act Amendments. Strictly speaking, some issues are interrelated and one issue cannot be completely dissociated from the others. However, this report addresses individual issues separately in order to determine all major aspects of bulk power system operations affected by each issue. The impacts of the five issues on power system reliability and security are summarized. This report examines the five critical issues that the US electric utility industry will be facing over the next decade. The investigation of their impacts on utility industry will be facing over the next decade. The investigation of their impacts on utility system reliability and security is limited to the system operation viewpoint. Those five issues will undoubtedly influence various planning aspects of the bulk transmission system. However, those subjects are beyond the scope of this report. While the issues will also influence the restructure and business of the utility industry politically, sociologically, environmentally, and economically, all discussion included in the report are focused only on technical ramifications.

  2. Dietary fish oil replacement by linseed oil: Effect on growth, nutrient utilization, tissue fatty acid composition and desaturase gene expression in silver barb (Puntius gonionotus) fingerlings.

    Science.gov (United States)

    Nayak, Madhusmita; Saha, Ashis; Pradhan, Avinash; Samanta, Mrinal; Giri, Shiba Shankar

    2017-03-01

    Silver barb (Puntius gonionotus) is considered a promising medium carp species for freshwater aquaculture in Asia. This study in silver barb was carried out to evaluate the effects of total or partial substitution of dietary fish oil (FO) with linseed oil (LO) on growth, nutrient utilization, whole-body composition, muscle and liver fatty acid composition. Fish (12.1±0.4g of initial body weight) were fed for 60days with five experimental iso-proteinous, iso-lipidic and iso-caloric diets in which FO (control diet) was replaced by 33.3%, 50%, 66.7% and 100% LO. Final weight, weight gain, percent weight gain, SGR decreased linearly (p0.05) affect the feed conversion ratio (FCR), protein efficiency ratio (PER) and whole body proximate composition. Furthermore, enhanced level of LO increased α-linolenic acid (ALA; 18:3n3) and linoleic acid (LA; 18:2n6) and decreased eicosapentaenoic acid (EPA; 20:5n3) and docosahexaenoic acid (DHA; 22:6n3) in muscle and liver. To understand the molecular mechanism of long chain-polyunsaturated fatty acid (LC-PUFA) biosynthesis, we cloned and characterized the fatty acyl Δ6 desaturase (Δ6 fad) cDNA and investigated its expression in various organs/tissues following replacement of FO with LO in the diet. The full-length Δ6 fad cDNA was 2056bp encoding 444 amino acids and was widely expressed in various organs/tissues. Replacement of FO with LO increased the expression of Δ6 fad mRNA in liver, muscle and intestine but no significant difference was found in the brain. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Utilizing cell-matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels.

    Science.gov (United States)

    Gojgini, Shiva; Tokatlian, Talar; Segura, Tatiana

    2011-10-01

    The effective delivery of DNA locally would increase the applicability of gene therapy in tissue regeneration, where diseased tissue is to be repaired in situ. One promising approach is to use hydrogel scaffolds to encapsulate and deliver plasmid DNA in the form of nanoparticles to the diseased tissue, so that cells infiltrating the scaffold are transfected to induce regeneration. This study focuses on the design of a DNA nanoparticle-loaded hydrogel scaffold. In particular, this study focuses on understanding how cell-matrix interactions affect gene transfer to adult stem cells cultured inside matrix metalloproteinase (MMP) degradable hyaluronic acid (HA) hydrogel scaffolds. HA was cross-linked to form a hydrogel material using a MMP degradable peptide and Michael addition chemistry. Gene transfer inside these hydrogel materials was assessed as a function of polyplex nitrogen to phosphate ratio (N/P = 5 to 12), matrix stiffness (100-1700 Pa), RGD (Arg-Gly-Asp) concentration (10-400 μM), and RGD presentation (0.2-4.7 RGDs per HA molecule). All variables were found to affect gene transfer to mouse mensenchymal stem cells culture inside the DNA loaded hydrogels. As expected, higher N/P ratios lead to higher gene transfer efficiency but also higher toxicity; softer hydrogels resulted in higher transgene expression than stiffer hydrogels, and an intermediate RGD concentration and RGD clustering resulted in higher transgene expression. We believe that the knowledge gained through this in vitro model can be utilized to design better scaffold-mediated gene delivery for local gene therapy.

  4. Important impacts of intestinal bacteria on utilization of dietary amino acids in pigs.

    Science.gov (United States)

    Yang, Yu-Xiang; Dai, Zhao-Lai; Zhu, Wei-Yun

    2014-11-01

    Bacteria in pig intestine can actively metabolize amino acids (AA). However, little research has focused on the variation in AA metabolism by bacteria from different niches. This study compared the metabolism of AA by microorganisms derived from the lumen and epithelial wall of the pig small intestine, aiming to test the hypothesis that the metabolic profile of AA by gut microbes was niche specific. Samples from the digesta, gut wall washes and gut wall of the jejunum and ileum were used as inocula. Anaerobic media containing single AA were used and cultured for 24 h. The 24-h culture served as inocula for the subsequent 30 times of subcultures. Results showed that for the luminal bacteria, all AA concentrations except phenylalanine in the ileum decreased during the 24-h in vitro incubation with a increase of ammonia concentration, while 4 AA (glutamate, glutamine, arginine and lysine) in the jejunum decreased, with the disappearance rate at 60-95 %. For tightly attached bacteria, all AA concentrations were generally increased during the first 12 h and then decreased coupled with first a decrease and then an increase of ammonia concentration, suggesting a synthesis first and then a catabolism pattern. Among them, glutamate in both segments, histidine in the jejunum and lysine in the ileum increased significantly during the first 12 h and then decreased at 24 h. The concentrations of glutamine and arginine did not change during the first 12 h, but significantly decreased at 24 h. Jejunal lysine and ileal threonine were increased for the first 6 or 12 h. For the loosely attached bacteria, there was no clear pattern for the entire AA metabolism. However, glutamate, methionine and lysine in the jejunum decreased after 24 h of cultivation, while glutamine and threonine in the jejunum and glutamine and lysine in the ileum increased in the first 12 h. During subculture, AA metabolism, either utilization or synthesis, was generally decreased with disappearance

  5. Planning of an Integrated Acidification Study and Survey on Acid Rain Impacts in China. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Lydersen, Espen; Angell, Valter; Eilertsen, Odd; Muniz, Ivar P. [Norsk Inst. for Naturforskning, Trondheim (Norway); Larssen, Thorbjoern; Seip, Hans Martin; Aagaard, Per; Vogt, Rolf D. [Oslo Univ. (Norway); Mulder, Jan

    1997-12-31

    This is the final report from the PIAC project, which was a multidisciplinary survey on acid rain in China. One goal was to document effects of airborne acidifying compounds on vegetation, soil, soil- and surface-water and aquatic biota. Other goals were to exchange knowledge between Chinese and Norwegian scientists, and to visit research sites in highly polluted areas in China and evaluate their need of support in a future collaborative monitoring and research programme. Samples have been collected from over 20 sites in three areas. Negative effects of air pollution are found on all ecosystem levels investigated. The concentration of sulfur in the air in urban and near-urban areas is very high. The concentration of volatile organic compounds is generally high, which means that increased NOx emissions in coming years may increase the ozone problems. Reduced photosynthesis activities were found in some plants and acidification observed in soil and surface water. Aquatic biota also reflect the acidification status of the surface waters investigated. However, it is difficult to assess the degree of damage in these regions because the survey includes too few sites. Surface water acidification is currently not a major environmental problem in China and is unlikely to be one during the next decades. The report includes a status report on acidification in China and a proposed framework for a monitoring programme based on Norwegian experiences. 139 refs., 16 figs., 45 tabs.

  6. 77 FR 75992 - Utility Scale Wind Towers From the People's Republic of China: Final Determination of Sales at...

    Science.gov (United States)

    2012-12-26

    ... coating, end-finish, painting, treatment, or method of manufacture, and with or without flanges, doors, or...., Polyethylene Terephthalate Film, Sheet, and Strip from the People's Republic of China: Final Determination of...

  7. Evaluation of Orange and Rockland Utilities, Inc.`s competitive bidding program for demand-side resources. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Peters, J.S.; Stucky, L.; Seratt, P.; Darden-Butler, D. [Barakat and Chamberlin, Inc., Portland, OR (United States)

    1993-02-01

    The process evaluation reports on the implementation of Orange and Rockland Utilities demand-side bidding program in New York State during 1991 and 1992. The program is implemented by two energy service companies in Orange and rockland`s New York State service territory. The process evaluation methodology included interviews with utility staff (3), energy service company staff (2), and participating (6) and nonparticipating (7) utility customers. The two energy service companies had enrolled 14 customers in the program by summer 1992. One company had achieved 90% of their 2.75 MW bid and the other had achieved less than 90% of their 6.9 MW bid. Critical factors in success were determination of a reasonable bid amount for the market and marketing to the appropriate customers. Customers most interested in the program included those with limited access to capital and medium-sized firms with poor cash flows, particularly schools and hospitals. The findings also show that due to the incentive structure and associated need for substantial customer contributions, lighting measures dominate all installations. Customers, however, were interested in the potential savings and six of the nonparticipants chose to either install measures on their own or enroll in the utility`s rebate program.

  8. D-erythroascorbic acid: Its preparations, chemistry, and metabolism (fungi and plants). Final report

    Energy Technology Data Exchange (ETDEWEB)

    Loewus, F.A. [Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry; Seib, P.A. [Kansas State Univ., Manhattan, KS (United States). Dept. of Grain Science and Industry

    1991-12-31

    The origin of oxalate in plants has received considerable attention and glycolate metabolism has been generally regarded as a prime precursor candidate although studies on the metabolism of L-ascorbic acid single out that plant constituent as well. Experiments with oxalate-accumulating plants that contain little or no tartaric acid revealed the presence of a comparable L-ascorbic acid metabolism with the exception that the cleavage products were oxalic acid and L-threonic acid or products of L-threonic acid metabolism. A reasonable mechanism for cleavage of L-ascorbic acid at the endiolic bond is found in studies on the photooxygenation of L-ascorbic acid. Presumably, analogs of L-ascorbic acid that differ only in the substituent at C4 also form a hydroperoxide in the presence of alkaline hydrogen peroxide and subsequently yield oxalic acid and the corresponding aldonic acid or its lactone. We became interested in such a possibility when we discovered that L-ascorbic acid was rare or absent in certain yeasts and fungi whereas a L-ascorbic acid analog, D-glycero-pent-2-enono- 1,4-lactone (D-erythroascorbic acid), was present. It has long been known that oxalate occurs in yeasts and fungi and its production plays a role in plant pathogenesis. As to the biosynthetic origin of fungal oxalic acid there is little information although it is generally assumed that oxaloacetate or possibly, glycolate, might be that precursor.

  9. Utilization of ornithine and arginine as specific precursors of clavulanic acid.

    OpenAIRE

    Romero, J; Liras, P; Martín, J F

    1986-01-01

    Ornithine and arginine (5 to 20 mM), but not glutamic acid or proline, exerted a concentration-dependent stimulatory effect on the biosynthesis of clavulanic acid in both resting-cell cultures and long-term fermentations of Streptomyces clavuligerus. Ornithine strongly inhibited cephamycin biosynthesis in the same strain. [1-14C]-, [5-14C]-, or [U-14 C] ornithine was efficiently incorporated into clavulanic acid, whereas the incorporation of uniformly labeled glutamic acid was very poor. [U-1...

  10. Yeast species utilizing uric acid, adenine, n-alkylamines or diamines as sole source of carbon and energy.

    Science.gov (United States)

    Middelhoven, W J; De Kievit, H; Biesbroek, A L

    1985-01-01

    Yeast strains utilizing uric acid, adenine, monoamines or diamines as sole source of carbon and energy were isolated from several soil samples by the enrichment culture method. The most common species was Trichosporon cutaneum. Strains of Candida catenulata, C. famata, C. parapsilosis, C. rugosa, Cryptococcus laurentii, Stephanoascus ciferrii and Tr. adeninovorans were also isolated. All strains utilizing uric acid as sole carbon source utilized some primary n-alkyl-l-amines, hydroxyamines or diamines as well. The ascomycetous yeast strains showing these characteristics all belonged to species known to assimilate hydrocarbons. Type strains of hydrocarbon-positive yeast species which were not found in the enrichment cultures generally assimilated putrescine, some type strains also butylamine or pentylamine, but none assimilated uric acid. Methanol-positive species were not isolated. Type strains of methanol-positive and of hydrocarbon-negative species did not assimilate uric acid, butylamine or putrescine. Assimilation of putrescine as sole source of carbon and energy may be a valuable diagnostic criterion in yeast taxonomy.

  11. Synergistic Utilization of Coal Fines and Municipal Solid Waste in Coal-Fired Boilers. Phase I Final Report

    Energy Technology Data Exchange (ETDEWEB)

    V. Zamansky; P. Maly; M. Klosky

    1998-06-12

    A feasibility study was performed on a novel concept: to synergistically utilize a blend of waste coal fines with so-called E-fuel for cofiring and reburning in utility and industrial boilers. The E-fuel is produced from MSW by the patented EnerTech's slurry carbonization process. The slurry carbonization technology economically converts MSW to a uniform, low-ash, low-sulfur, and essentially chlorine-free fuel with energy content of about 14,800 Btu/lb.

  12. Utilization of ornithine and arginine as specific precursors of clavulanic acid.

    Science.gov (United States)

    Romero, J; Liras, P; Martín, J F

    1986-01-01

    Ornithine and arginine (5 to 20 mM), but not glutamic acid or proline, exerted a concentration-dependent stimulatory effect on the biosynthesis of clavulanic acid in both resting-cell cultures and long-term fermentations of Streptomyces clavuligerus. Ornithine strongly inhibited cephamycin biosynthesis in the same strain. [1-14C]-, [5-14C]-, or [U-14 C] ornithine was efficiently incorporated into clavulanic acid, whereas the incorporation of uniformly labeled glutamic acid was very poor. [U-14C] citrulline were not incorporated at all. Mutant nca-1, a strain that is blocked in clavulanic acid biosynthesis, did not incorporate arginine into clavulanic acid. S. clavuligerus showed arginase activity, converting arginine into ornithine, but not amidinotransferase activity. Both arginase activity and clavulanic acid formation were enhanced simultaneously by supplementing the production medium with 10 mM arginine. PMID:2877616

  13. Utilization of acidic α-amino acids as acyl donors: an effective stereo-controllable synthesis of aryl-keto α-amino acids and their derivatives.

    Science.gov (United States)

    Wang, Lei; Murai, Yuta; Yoshida, Takuma; Okamoto, Masashi; Tachrim, Zetryana Puteri; Hashidoko, Yasuyuki; Hashimoto, Makoto

    2014-05-16

    Aryl-keto-containing α-amino acids are of great importance in organic chemistry and biochemistry. They are valuable intermediates for the construction of hydroxyl α-amino acids, nonproteinogenic α-amino acids, as well as other biofunctional components. Friedel-Crafts acylation is an effective method to prepare aryl-keto derivatives. In this review, we summarize the preparation of aryl-keto containing α-amino acids by Friedel-Crafts acylation using acidic α-amino acids as acyl-donors and Lewis acids or Brönsted acids as catalysts.

  14. Utilization of geothermal energy for methane production for J. A. Albertson Land and Cattle Company. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1980-07-01

    The feasibility of an integrated system to utilize a geothermal resource for a bioconversion plant. This integrated facility would use the manure from approximately 30,000 head of feedlot cattle as a feedstock for an anaerobic digestion plant. The findings on engineering design, geological assessment, environmental, economic, and institutional requirements of the proposed project are summarized. (MHR)

  15. Engineering and economic analysis for the utilization of geothermal fluids in a cane sugar processing plant. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Humme, J.T.; Tanaka, M.T.; Yokota, M.H.; Furumoto, A.S.

    1979-07-01

    The purpose of this study was to determine the feasibility of geothermal resource utilization at the Puna Sugar Company cane sugar processing plant, located in Keaau, Hawaii. A proposed well site area was selected based on data from surface exploratory surveys. The liquid dominated well flow enters a binary thermal arrangement, which results in an acceptable quality steam for process use. Hydrogen sulfide in the well gases is incinerated, leaving sulfur dioxide in the waste gases. The sulfur dioxide in turn is recovered and used in the cane juice processing at the sugar factory. The clean geothermal steam from the binary system can be used directly for process requirements. It replaces steam generated by the firing of the waste fibrous product from cane sugar processing. The waste product, called bagasse, has a number of alternative uses, but an evaluation clearly indicated it should continue to be employed for steam generation. This steam, no longer required for process demands, can be directed to increased electric power generation. Revenues gained by the sale of this power to the utility, in addition to other savings developed through the utilization of geothermal energy, can offset the costs associated with hydrothermal utilization.

  16. Utilization of cotton plant ash and char for removal of 2, 4-dichlorophenoxyacetic acid

    Directory of Open Access Journals (Sweden)

    Nikhilesh S. Trivedi

    2016-12-01

    Full Text Available Cotton is a common Indian crop grown on a considerable portion of farmland across the country. After separating the useful product (cotton fibers, the other parts of the plant (stalks, leaves, etc. are discarded as wastes. In most cases, these plant materials are used as fuel in boilers or households. Cotton wastes when ignited in the presence and absence of air produce cotton plant ash (CPA and cotton plant char (CPC, respectively. However, CPA and CPC produced pose environmental problems such as safe disposal. Thus, there is an urgent need to characterize the physical and chemical properties of these derivatives and to identify their potential uses. This study highlights the potential utilization of CPA and CPC as adsorbents of 2,4-D. The main components in CPA, namely, CaO and K2O, provide micronutrients to the soil and are thus useful as a biofertilizers. Moreover, low manufacturing cost and higher availability favor the use of CPA as an efficient, low-cost adsorbent as well as a potential source of vital micronutrients. The adsorption capacity of CPA and CPC was tested using 2,4 dichlorophenoxyacetic acid (2,4-D as the representative herbicide. Experimental data were analyzed by Freundlich and Langmuir adsorption isotherms, and these fitted well with the Langmuir model. The adsorption capacity q0 was found to be 0.64 mg/g for CPA and 3.93 mg/g for CPC. Pseudo-first-order pseudo-second-order and intraparticle diffusion kinetic models were applied to experimental data, and the pseudo second order kinetics model showed best fit for the adsorption of 2,4-D on CPA and CPC. Both CPA and CPC were characterized using proximate analysis, SEM images, BET surface area, XRF, FTIR, and CHNS. The BET surface area was found to be 2 and 109 m2/g, respectively, for CPA and CPC. Adsorption study results indicated that both CPA and CPC are very effective cheap adsorbent for 2,4-D removal.

  17. The utility of uric acid assay in dogs as an indicator of functional hepatic mass

    Directory of Open Access Journals (Sweden)

    J. M. Hill

    2011-04-01

    Full Text Available Uric acid was used as a test for liver disease before the advent of enzymology. Three old studies criticised uric acid as a test of liver function. Uric acid, as an end-product of purine metabolism in the liver, deserved re-evaluation as a liver function test. Serumtotal bile acids are widely accepted as the most reliable liver function test. This study compared the ability of serumuric acid concentration to assess liver function with that of serumpre-prandial bile acids in dogs. In addition, due to the renal excretion of uric acid the 2 assays were also compared in a renal disease group. Using a control group of healthy dogs, a group of dogs with congenital vascular liver disease, a group of dogs with non-vascular parenchymal liver diseases and a renal disease group, the ability of uric acid and pre-prandial bile acids was compared to detect reduced functional hepatic mass overall and in the vascular or parenchymal liver disease groups separately. Sensitivities, specificities and predictive value parameters were calculated for each test. The medians of uric acid concentration did not differ significantly between any of the groups, whereas pre-prandial bile acids medians were significantly higher in the liver disease groups compared with the normal and renal disease group of dogs. The sensitivity of uric acid in detecting liver disease overall was 65% while the specificity of uric acid in detecting liver disease overall was 59 %. The sensitivity and specificity of uric acid in detecting congenital vascular liver disease was 68%and 59 %, respectively. The sensitivity and specificity of uric acid in detecting parenchymal liver disease was 63%and 60 %, respectively. The overall positive and negative predictive values for uric acid in detecting liver disease were poor and the data in this study indicated uric acid to be an unreliable test of liver function. In dogs suffering from renal compromise serum uric acid concentrations may increase into the

  18. Utilization of oriented crystal growth for screening of aromatic carboxylic acids cocrystallization with urea

    Science.gov (United States)

    Przybyłek, Maciej; Ziółkowska, Dorota; Kobierski, Mirosław; Mroczyńska, Karina; Cysewski, Piotr

    2016-01-01

    The possibility of molecular complex formation in the solid state of urea with benzoic acid analogues was measured directly on the crystallite films deposited on the glass surface using powder X-ray diffractometry (PXRD). Obtained solid mixtures were also analyzed using Fourier transform infrared spectroscopy (FTIR). The simple droplet evaporation method was found to be efficient, robust, fast and cost-preserving approach for first stage cocrystal screening. Additionally, the application of orientation effect to cocrystal screening simplifies the analysis due to damping of majority of diffraction signals coming from coformers. During validation phase the proposed approach successfully reproduced both positive cases of cocrystallization (urea:salicylic acid and urea:4-hydroxy benzoic acid) as well as pairs of co-formers immiscible in the solid state (urea:benzoic acid and urea:acetylsalicylic acids). Based on validated approach new cocrystals of urea were identified in complexes with 3-hydroxybenzoic acid, 2,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,6-dihydroxybenzoic acid and 3,5-dihydroxybenzoic acid. In all cases formation of multicomponent crystal phase was confirmed by the appearance of new reflexes on the diffraction patterns and FTIR absorption band shifts of O-H and N-H groups.

  19. Omega-3 fatty acids and coronary heart disease. The final verdict?

    NARCIS (Netherlands)

    Kromhout, D.

    2012-01-01

    Purpose of review: The fish fatty acids eicosapentenoic acid (EPA) and docosahexenoic acid (DHA) may be protective against fatal coronary heart disease (CHD) and sudden cardiac death. This review summarizes the recent findings of prospective cohort studies and randomized controlled trials. Recent fi

  20. Nitrogen in dietary glutamate is utilized exclusively for the synthesis of amino acids in the rat intestine.

    Science.gov (United States)

    Nakamura, Hidehiro; Kawamata, Yasuko; Kuwahara, Tomomi; Torii, Kunio; Sakai, Ryosei

    2013-01-01

    Although previous studies have shown that virtually the entire carbon skeleton of dietary glutamate (glutamate-C) is metabolized in the gut for energy production and amino acid synthesis, little is known regarding the fate of dietary glutamate nitrogen (glutamate-N). In this study, we hypothesized that dietary glutamate-N is an effective nitrogen source for amino acid synthesis and investigated the fate of dietary glutamate-N using [(15)N]glutamate. Fischer male rats were given hourly meals containing [U-(13)C]- or [(15)N]glutamate. The concentration and isotopic enrichment of several amino acids were measured after 0-9 h of feeding, and the net release of each amino acid into the portal vein was calculated. Most of the dietary glutamate-C was metabolized into CO(2), lactate, or alanine (56, 13, and 12% of the dietary input, respectively) in the portal drained viscera (PDV). Most of the glutamate-N was utilized for the synthesis of other amino acids such as alanine and citrulline (75 and 3% of dietary input, respectively) in the PDV, and only minor amounts were released into the portal vein in the form of ammonia and glutamate (2 and 3% of the dietary input, respectively). Substantial incorporation of (15)N into systemic amino acids such as alanine, glutamine, and proline, amino acids of the urea cycle, and branched-chain amino acids was also evident. These results provide quantitative evidence that dietary glutamate-N distributes extensively to amino acids synthesized in the PDV and, consequently, to circulating amino acids.

  1. Effect of Wheat Middlings, Microbial Phytase, and Citric Acid on Phytate-Phosphorus, Calcium, and Protein Utilization of Broilers

    Institute of Scientific and Technical Information of China (English)

    GONG Yi-feng; LIAO He-rong; WANG Jin-fu; LI Hong-yan

    2006-01-01

    A corn-soybean meal diet (CSB) (or Diet 1) containing 23% crude protein (CP) was used as the positive control, and another corn-soybean meal diet containing 21% CP and 15% wheat middlings (WM) (or Diet 2) was used as the basal diet, which was treated with four different treatments. Digestibility experiment was employed to discuss the collective effect of citric acid, and intrinsic and microbial phytase. By comparing and analyzing effects of them in the low-nutrient broiler diets, the results showed five treatments had similar effects on Tibia ash (%) (mg) (P > 0.05). Under the supplementation of bacterial phytase or citric acid, the daily body weight gain (ADG), gain:feed (G:F) ratio, and calcium (Ca) utilization were similar to that of standard-nutrient CSB diet (Diet 1) (P > 0.05). And, fecal phosphorus (P) and CP utilization were lower than (P < 0.05) that of Diet 1. But P utilization was significantly higher than (P < 0.01) that of Diet 1.However, the ADG, G:F, and CP utilization produced by supplementation of intrinsic phytase were lower than those of Diet 1, but other aspects were similar to those produced by Diet 1 (P > 0.05). In Diet 5, citric acid, intrinsic and bacterial phytase were added to the diet, which produced a 1.4% decrease on fecal P, a 7.2% increase on Ca utilization, which was significantly higher than (P < 0.01) those of the other four Diets, a 3.9% increase on G:F, which was similar to that of Diet 1, and a 2.3% increase on CP utilization, which was higher than (P < 0.05) that of the other three diets. In summary, the results of this study indicated that citric acid, intrinsic and bacterial phytase might have some additive or synergistic effects, and low-nutrient CSB diets with 15% wheat middlings, 750 U kg-1 phytase, and 3% citric acid might substitute completely for standard CSB in broilers.

  2. SOLID AND LIQUID PINEAPPLE WASTE UTILIZATION FOR LACTIC ACID FERMENTATION USING Lactobacillus delbrueckii

    OpenAIRE

    Abdullah Abdullah

    2012-01-01

    The liquid and solid  pineapple wastes contain mainly sucrose, glucose, fructose and other nutrients. It therefore can potentially be used as carbon source for fermentation to produce organic acid. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer. The experiments were  carried out in batch fermentation using  the  liquid and solid pineapple wastes to produce lactic acid. The anaerobic fermentation of ...

  3. High efficient treatment of citric acid effluent by Chlorella vulgaris and potential biomass utilization.

    Science.gov (United States)

    Li, Changling; Yang, Hailin; Xia, Xiaole; Li, Yuji; Chen, Luping; Zhang, Meng; Zhang, Ling; Wang, Wu

    2013-01-01

    The efficiency of treating citric acid effluent by green algae Chlorella was investigated. With the highest growth rate, Chlorella vulgaris C9-JN2010 that could efficiently remove nutrients in the citric acid effluent was selected for scale-up batch experiments under the optimal conditions, where its maximum biomass was 1.04 g l(-1) and removal efficiencies of nutrients (nitrogen, phosphorus, total organic carbon, chemical oxygen demand and biochemical oxygen demand) were above 90.0%. Algal lipid and protein contents were around 340.0 and 500.0 mg · g(-1) of the harvested biomass, respectively. Proportions of polyunsaturated fatty acids in the lipids and eight kinds of essential amino acids in algal protein were 74.0% and 40.0%, respectively. Three major fatty acids were hexadecanoic acid, eicosapentaenoic acid and docosadienoic acid. This specific effluent treatment process could be proposed as a dual-beneficial approach, which converts nutrients in the high strength citric acid effluent into profitable byproducts and reduces the contaminations.

  4. Provision of utility support services to the US Department of Energy San Francisco Operations Office. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The main purpose of this project was to provide to DOE/SAN continuing, follow-up support to realize savings from a number of alternate supply arrangements that had already been and/or were expected to be identified under the original project. This expected continuation of these efforts is demonstrated by certain of the tasks that are spelled out in the Statement of Work. For example: Evaluate and propose alternative options and methods for improving efficiency, reducing cost, and making effective use of the energy supplies and facilities under various conditions of use; Provide engineering and economic analysis and recommendations for utility-related facilities and service issues, such as high voltage discounts, ownership of facilities, etc.; Assist in developing strategy and documentation in support of negotiating utility contracts and modifications thereto. In addition, the follow-on contract provided for monitoring and intervening in rate cases that had particular relevance to the DOE/SAN laboratories.

  5. Human ketone body production and utilization studied using tracer techniques: Regulation by free fatty acids, insulin, catecholamines, and thyroid hormones

    Energy Technology Data Exchange (ETDEWEB)

    Keller, U.; Lustenberger, M.; Mueller-Brand, J.G.; Gerber, P.P.; Stauffacher, W.

    1989-05-01

    Ketone body concentrations fluctuate markedly during physiological and pathological conditions. Tracer techniques have been developed in recent years to study production, utilization, and the metabolic clearance rate of ketone bodies. This review describes data on the roles of insulin, catecholamines, and thyroid hormones in the regulation of ketone body kinetics. The data indicate that insulin lowers ketone body concentrations by three independent mechanisms: first, it inhibits lipolysis, and thus lowers free fatty acid availability for ketogenesis; second, it restrains ketone body production within the liver; third, it enhances peripheral ketone body utilization. To assess these effects in humans in vivo, experimental models were developed to study insulin effects with controlled concentrations of free fatty acids, insulin, glucagon, and ketone bodies. Presently available data also support an important role of catecholamines in increasing ketone body concentrations. Evidence was presented that norepinephrine increases ketogenesis not only by stimulating lipolysis, and thus releasing free fatty acids, but also by increasing intrahepatic ketogenesis. Thyroid hormone availability was associated with lipolysis and ketogenesis. Ketone body concentrations after an overnight fast were only modestly elevated in hyperthyroidism resulting from increased peripheral ketone body clearance. There was a significant correlation between serum triiodothyronine levels and the ketone body metabolic clearance rate. Thus, ketone body homeostasis in human subjects resulted from the interaction of hormones such as insulin, catecholamines, and thyroid hormones regulating lipolysis, intrahepatic ketogenesis, and peripheral ketone body utilization. 58 references.

  6. Utilization of CO2 fixating bacterium Actinobacillus succinogenes 130Z for simultaneous biogas upgrading and bio-succinic acid production

    DEFF Research Database (Denmark)

    Gunnarsson, Ingólfur Bragi; Alvarado-Morales, Merlin; Angelidaki, Irini

    2014-01-01

    acid using the bacterial strain Actinobacillus succinogenes 130Z, and simultaneously producing high purity CH4 (>95%). Results showed that when pressure during fermentation was increased from 101.325 to 140 kPa, higher CO2 solubility was achieved, thereby positively affecting final succinic acid yield...... and titre, CO2 consumption rate and CH4 purity. When using biogas as the only CO2 source at 140 kPa, the CO2 consumption rate corresponded to 2.59 L CO2 L-1 d-1 with a final succinic acid titre of 14.4 g L-1. Under this pressure condition the highest succinic acid yield and biogas quality reached...... corresponded to 0.635 g g-1 and 95.4% (v v-1) CH4 content respectively after 24 hours fermentation. This work represents the first successful attempt to develop a system capable of upgrading biogas to vehicle fuel/gas grid quality and simultaneously produce bio-succinic acid, a valuable building block...

  7. Utilization of CO2 fixating bacterium Actinobacillus succinogenes 130Z for simultaneous biogas upgrading and biosuccinic acid production.

    Science.gov (United States)

    Gunnarsson, Ingólfur B; Alvarado-Morales, Merlin; Angelidaki, Irini

    2014-10-21

    Biogas is an attractive renewable energy carrier. However, it contains CO2 which limits its use for certain applications. Here we report a novel approach for removing CO2 from biogas and capturing it as a biochemical through a biological process. This approach entails converting CO2 into biosuccinic acid using the bacterial strain Actinobacillus succinogenes 130 Z, and simultaneously producing high-purity CH4 (> 95%). Results showed that when pressure during fermentation was increased from 101.325 to 140 kPa, higher CO2 solubility was achieved, thereby positively affecting final succinic acid yield and titer, CO2 consumption rate, and CH4 purity. When using biogas as the only CO2 source at 140 kPa, the CO2 consumption rate corresponded to 2.59 L CO2 L(-1) d(-1) with a final succinic acid titer of 14.4 g L(-1). Under this pressure condition, the highest succinic acid yield and biogas quality reached corresponded to 0.635 g g(-1) and 95.4% (v v(-1)) CH4 content, respectively, after 24 h fermentation. This work represents the first successful attempt to develop a system capable of upgrading biogas to vehicle fuel/gas grid quality and simultaneously produce biosuccinic acid, a valuable building block with large market potential in the near term.

  8. FadD is required for utilization of endogenous fatty acids released from membrane lipids.

    Science.gov (United States)

    Pech-Canul, Ángel; Nogales, Joaquina; Miranda-Molina, Alfonso; Álvarez, Laura; Geiger, Otto; Soto, María José; López-Lara, Isabel M

    2011-11-01

    FadD is an acyl coenzyme A (CoA) synthetase responsible for the activation of exogenous long-chain fatty acids (LCFA) into acyl-CoAs. Mutation of fadD in the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti promotes swarming motility and leads to defects in nodulation of alfalfa plants. In this study, we found that S. meliloti fadD mutants accumulated a mixture of free fatty acids during the stationary phase of growth. The composition of the free fatty acid pool and the results obtained after specific labeling of esterified fatty acids with a Δ5-desaturase (Δ5-Des) were in agreement with membrane phospholipids being the origin of the released fatty acids. Escherichia coli fadD mutants also accumulated free fatty acids released from membrane lipids in the stationary phase. This phenomenon did not occur in a mutant of E. coli with a deficient FadL fatty acid transporter, suggesting that the accumulation of fatty acids in fadD mutants occurs inside the cell. Our results indicate that, besides the activation of exogenous LCFA, in bacteria FadD plays a major role in the activation of endogenous fatty acids released from membrane lipids. Furthermore, expression analysis performed with S. meliloti revealed that a functional FadD is required for the upregulation of genes involved in fatty acid degradation and suggested that in the wild-type strain, the fatty acids released from membrane lipids are degraded by β-oxidation in the stationary phase of growth.

  9. Recovery of calcium carbonate from steelmaking slag and utilization for acid mine drainage pre-treatment

    CSIR Research Space (South Africa)

    Mulopo, J

    2012-06-01

    Full Text Available the technical feasibility of calcium carbonate recovery and its use for pre-treatment of Acid Mine Drainage (AMD) from coal mines. The effect of key process parameters, such as the amount of acid (HCl/Calcium molar ratio), the pH and the CO2 flow rate were...

  10. Utilization of Glial Fibrillary Acidic Protein and Galectin-3 in the ...

    African Journals Online (AJOL)

    2017-05-16

    May 16, 2017 ... in clinically suspected ischemic stroke patients with normal computerized cranial tomography ... of Galectin-3 and GFAP were increased in acute ischemic stroke patients. Utilization of ..... Bench-to-bedside review: Outcome.

  11. Control of amphibious weed ipomoea (Ipomoea carnea by utilizing it for the extraction of volatile fatty acids as energy precursors

    Directory of Open Access Journals (Sweden)

    M. Rafiq Kumar

    2015-01-01

    Full Text Available Volatile fatty acids (VFAs, comprising mainly of acetic acid and lesser quantities of propionic and butyric acids, are generated when zoomass or phytomass is acted upon by acidogenic and acetogenic microorganisms. VFAs can be utilized by methanogens under anaerobic conditions to generate flammable methane–carbon dioxide mixtures known as ‘biogas’. Acting on the premise that this manner of VFA utilization for generating relatively clean energy can be easily accomplished in a controlled fashion in conventional biogas plants as well as higher-rate anaerobic digesters, we have carried out studies aimed to generate VFAs from the pernicious weed ipomoea (Ipomoea carnea. The VFA extraction was accomplished by a simple yet effective technology, appropriate for use even by laypersons. For this acid-phase reactors were set, to which measured quantities of ipomoea leaves were charged along with water inoculated with cow dung. The reactors were stirred intermittently. It was found that VFA production started within hours of the mixing of the reactants and peaked by the 10th or 11th day in all the reactors, effecting a conversion of over 10% of the biomass into VFAs. The reactor performance had good reproducibility and the process appeared easily controllable, frugal and robust.

  12. Expression of genes participating in regulation of fatty acid and glucose utilization and energy metabolism in developing rat hearts.

    Science.gov (United States)

    Lavrentyev, Eduard N; He, Daifen; Cook, George A

    2004-11-01

    The heart is a unique organ that can use several fuels for energy production. During development, the heart undergoes changes in fuel supply, and it must be able to respond to these changes. We have examined changes in the expression of several genes that regulate fuel transport and metabolism in rat hearts during early development. At birth, there was increased expression of fatty acid transporters and enzymes of fatty acid metabolism that allow fatty acids to become the major source of energy for cardiac muscle during the first 2 wk of life. At the same time, expression of genes that control glucose transport and oxidation was downregulated. After 2 wk, expression of genes for glucose uptake and oxidation was increased, and expression of genes for fatty acid uptake and utilization was decreased. Expression of carnitine palmitoyltransferase I (CPT I) isoforms during development was different from published data obtained from rabbit hearts. CPT Ialpha and Ibeta isoforms were both highly expressed in hearts before birth, and both increased further at birth. Only after the second week did CPT Ialpha expression decrease appreciably below the level of CPT Ibeta expression. These results represent another example of different expression patterns of CPT I isoforms among various mammalian species. In rats, changes in gene expression followed nutrient availability during development and may render cardiac fatty acid oxidation less sensitive to factors that influence malonyl-CoA content (e.g., fluctuations in glucose concentration) and thereby favor fatty acid oxidation as an energy source for cardiomyocytes in early development.

  13. RDI's Wisdom Way Solar Village Final Report: Includes Utility Bill Analysis of Occupied Homes

    Energy Technology Data Exchange (ETDEWEB)

    Robb Aldrich, Steven Winter Associates

    2011-07-01

    In 2010, Rural Development, Inc. (RDI) completed construction of Wisdom Way Solar Village (WWSV), a community of ten duplexes (20 homes) in Greenfield, MA. RDI was committed to very low energy use from the beginning of the design process throughout construction. Key features include: 1. Careful site plan so that all homes have solar access (for active and passive); 2. Cellulose insulation providing R-40 walls, R-50 ceiling, and R-40 floors; 3. Triple-pane windows; 4. Airtight construction (~0.1 CFM50/ft2 enclosure area); 5. Solar water heating systems with tankless, gas, auxiliary heaters; 6. PV systems (2.8 or 3.4kWSTC); 7. 2-4 bedrooms, 1,100-1,700 ft2. The design heating loads in the homes were so small that each home is heated with a single, sealed-combustion, natural gas room heater. The cost savings from the simple HVAC systems made possible the tremendous investments in the homes' envelopes. The Consortium for Advanced Residential Buildings (CARB) monitored temperatures and comfort in several homes during the winter of 2009-2010. In the Spring of 2011, CARB obtained utility bill information from 13 occupied homes. Because of efficient lights, appliances, and conscientious home occupants, the energy generated by the solar electric systems exceeded the electric energy used in most homes. Most homes, in fact, had a net credit from the electric utility over the course of a year. On the natural gas side, total gas costs averaged $377 per year (for heating, water heating, cooking, and clothes drying). Total energy costs were even less - $337 per year, including all utility fees. The highest annual energy bill for any home evaluated was $458; the lowest was $171.

  14. Direct utilization of geothermal energy resources in food processing. Final report, May 17, 1978-May 31, 1982

    Energy Technology Data Exchange (ETDEWEB)

    Austin, J.C.

    1982-05-01

    In early 1978 financial assistance was granted for a project to utilize geothermal energy at Ore-Ida Foods, Inc.'s food processing plant in Ontario, Oregon. Specifically, the project included exploring, testing, and developing the potential geothermal resource; retrofitting the existing gas/oil-fired steam system; utilizing the geothermal resource for food processing, space heating, and hot potable water; and injecting the spent geothermal water back into a disposal well. Based on preliminary investigations which indicated the presence of a local geothermal resource, drilling began in August 1979. Although the anticipated resource temperature of 380/sup 0/F was reached at total well depth (10,054 feet), adequate flow to meet processing requirements could not be obtained. Subsequent well testing and stimulation techniques also failed to produce the necessary flow, and the project was eventually abandoned. However, throughout the duration of the project, all activities were carefully monitored and recorded to ensure the program's value for future evaluation. This report presents a culmination of data collected during the Ore-Ida project.

  15. Direct utilization of geothermal resources field experiments at Monroe, Utah. Final report, July 14, 1978-July 13, 1981

    Energy Technology Data Exchange (ETDEWEB)

    Blair, C.K.; Owen, L.B. (eds.)

    1982-12-01

    The City of Monroe, Utah undertook a project to demonstrate the economic and technical viability of utilizing a low temperature geothermal resource to provide space and hot water heating to commercial, municipal, and domestic users within the community. During the course of the project, resource development and assessment, including drilling of a production well, was successfully completed. Upon completion of the field development and assessment phase of the program and of a preliminary design of the district heating system, it was determined that the project as proposed was not economically viable. This was due to: (1) a significant increase in estimated capital equipment costs resulting from the general inflation in construction costs, the large area/low population density in Monroe, and a more remote fluid disposal well site than planned, could not balance increased construction costs, (2) a lower temperature resource than predicted, and (3) due to predicted higher pumping and operating costs. After a thorough investigation of alternatives for utilizing the resource, further project activities were cancelled because the project was no longer economical and an alternative application for the resource could not be found within the constraints of the project. The City of Monroe, Utah is still seeking a beneficial use for the 600 gpm, 164/sup 0/F geothermal well. A summary of project activities included.

  16. Individual and structural environmental influences on utilization of iron and folic acid supplementation among pregnant women in Harare, Zimbabwe.

    Science.gov (United States)

    Tinago, Chiwoneso B; Annang Ingram, Lucy; Blake, Christine E; Frongillo, Edward A

    2017-07-01

    Micronutrient deficiencies are prevalent among Zimbabweans with serious health and social implications. Due to a lack of a national micronutrient food fortification policy, the Zimbabwe Ministry of Health and Child Care established a policy for the prevention of maternal micronutrient deficiencies, which centres on pregnant women receiving daily iron and folic acid (IFA) at their first antenatal care visit and throughout pregnancy. Despite these efforts, utilization of IFA supplementation in pregnancy in Zimbabwe is low. This study aimed to understand the experiences and knowledge of IFA supplementation among pregnant women and healthcare workers in Harare, Zimbabwe, and the influence of health-service and social environments on utilization. Semi-structured in-depth interviews were conducted in Shona and English, with pregnant women (n = 24) and healthcare workers (n = 14) providing direct antenatal care services to pregnant women in two high-density community clinics. Data were analysed thematically using NVivo 10. Influences on utilization were at the individual and structural environmental levels. Reasons for low utilization of IFA supplementation included forgetting to take IFA, side effects, misconceptions about IFA, limited access to nutrition information, delayed entry or non-uptake of antenatal care and social norms of pregnant women for IFA supplementation. Utilization was enhanced by knowledge of risks and benefits of supplementation, fear of negative health complications with non-utilization, family support and healthcare worker recommendation for supplementation. Study findings can inform approaches to strengthen micronutrient supplementation utilization to improve the micronutrient status of pregnant women to decrease maternal mortality and improve overall maternal and child health in Zimbabwe. © 2016 John Wiley & Sons Ltd. © 2016 John Wiley & Sons Ltd.

  17. Construction of energy-conserving sucrose utilization pathways for improving poly-γ-glutamic acid production in Bacillus amyloliquefaciens.

    Science.gov (United States)

    Feng, Jun; Gu, Yanyan; Quan, Yufen; Gao, Weixia; Dang, Yulei; Cao, Mingfeng; Lu, Xiaoyun; Wang, Yi; Song, Cunjiang; Wang, Shufang

    2017-06-06

    Sucrose is an naturally abundant and easily fermentable feedstock for various biochemical production processes. By now, several sucrose utilization pathways have been identified and characterized. Among them, the pathway consists of sucrose permease and sucrose phosphorylase is an energy-conserving sucrose utilization pathway because it consumes less ATP when comparing to other known pathways. Bacillus amyloliquefaciens NK-1 strain can use sucrose as the feedstock to produce poly-γ-glutamic acid (γ-PGA), a highly valuable biopolymer. The native sucrose utilization pathway in NK-1 strain consists of phosphoenolpyruvate-dependent phosphotransferase system and sucrose-6-P hydrolase and consumes more ATP than the energy-conserving sucrose utilization pathway. In this study, the native sucrose utilization pathway in NK-1 was firstly deleted and generated the B. amyloliquefaciens 3Δ strain. Then four combination of heterologous energy-conserving sucrose utilization pathways were constructed and introduced into the 3Δ strain. Results demonstrated that the combination of cscB (encodes sucrose permease) from Escherichia coli and sucP (encodes sucrose phosphorylase) from Bifidobacterium adolescentis showed the highest sucrose metabolic efficiency. The corresponding mutant consumed 49.4% more sucrose and produced 38.5% more γ-PGA than the NK-1 strain under the same fermentation conditions. To our best knowledge, this is the first report concerning the enhancement of the target product production by introducing the heterologous energy-conserving sucrose utilization pathways. Such a strategy can be easily extended to other microorganism hosts for reinforced biochemical production using sucrose as substrate.

  18. Utilization of glial fibrillary acidic protein and galectin-3 in the ...

    African Journals Online (AJOL)

    Results: A total of 90 participants, forty patients with ischemic infarction who ... Conclusion: The levels of Galectin-3 and GFAP were increased in acute ischemic stroke patients. Keywords: Galectin-3, glial fibrillary acidic protein, ischemic stroke ...

  19. Production development and utilization of Zimmer Station wet FGD by-products. Final report. Volume 1, Executive summary

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Kevin [Dravo Technology Center, Pittsburgh, PA (United States); Beeghly, Joel H. [Dravo Technology Center, Pittsburgh, PA (United States)

    2000-11-30

    About 30 electric utility units with a combined total of 15,000 MW utilize magnesium enhanced lime flue gas desulfurization (FGD) systems. A disadvantage of this and other inhibited or natural oxidation wet FGD systems is the capital and operating cost associated with landfill disposal of the calcium sulfite based solids. Fixation to stabilize the solids for compaction in a landfill also consumes fly ash that otherwise may be marketable. This Executive Summary describes efforts to dewater the magnesium hydroxide and gypsum slurries and then process the solids into a more user friendly and higher value form. To eliminate the cost of solids disposal in its first generation Thiosorbic® system, the Dravo Lime Company developed the ThioClear® process that utilizes a magnesium based absorber liquor to remove S02 with minimal suspended solids. Magnesium enhanced lime is added to an oxidized bleed stream of thickener overflow (TOF) to produce magnesium hydroxide [Mg(OH)2] and gypsum (CaS04 • 2H20), as by-products. This process was demonstrated at the 3 to 5 MW closed loop FGD system pilot plant at the Miami Fort Station of Cinergy, near Cincinnati, Ohio with the help of OCDO Grant Agreement CDO/D-91-6. A similar process strictly for'recovery and reuse of Mg(OH)2 began operation at the Zimmer Station of Cinergy in late 1994 that can produce 900 pounds of Mg(OH)2 per hour and 2,600 pounds of gypsum per hour. This by-product plant, called the Zimmer Slipstream Magnesium Hydroxide Recovery Project Demonstration, was conducted with the help of OCDO Grant Agreement CDO/D-921-004. Full scale ThioClear® plants began operating in 1997 at the 130 MW Applied Energy Services plant, in Monaca, PA, and in year 2000 at the 1,330 MW Allegheny Energy Pleasants Station at St. Marys, WV.

  20. Operational, cost, and technical study of large windpower systems integrated with an existing electric utility. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Ligon, C.; Kirby, G.; Jordan, D.; Lawrence, J.H.; Wiesner, W.; Kosovec, A.; Swanson, R.K.; Smith, R.T.; Johnson, C.C.; Hodson, H.O.

    1976-04-01

    Detailed wind energy assessment from the available wind records, and evaluation of the application of wind energy systems to an existing electric utility were performed in an area known as the Texas Panhandle, on the Great Plains. The study area includes parts of Texas, eastern New Mexico, the Oklahoma Panhandle and southern Kansas. The region is shown to have uniformly distributed winds of relatively high velocity, with average wind power density of 0.53 kW/m/sup 2/ at 30 m height at Amarillo, Texas, a representative location. The annual period of calm is extremely low. Three separate compressed air storage systems with good potential were analyzed in detail, and two potential pumped-hydro facilities were identified and given preliminary consideration. Aquifer storage of compressed air is a promising possibility in the region.

  1. Effect of coal quality on maintenance costs at utility plants. Final report. [Effect of ash and sulfur content of coal

    Energy Technology Data Exchange (ETDEWEB)

    Holt, E.C. Jr.

    1980-06-01

    In an attempt to determine if correlation exists between coal quality, as measured by its ash and sulfur contents, and the maintenance cost at utility plants, an examination was made of the actual maintenance cost experience of selected portions of five TVA coal-fired power plants as a function of the fuel quality consumed during an extended period of time. The results indicate that, according to our decision rules developed in compliance with accepted statistical practices, correlation does exist in many portions of the coal-fired plants for which sufficient maintenance cost records were available. The degree of correlation varies significantly among the individual portions of a particular plant as well as among the various plants. However, the indicators are sufficient to confirm that a change (within the design constraints of the unit) in the ash and/or sulfur content of the coal being consumed by a utility boiler will have a proportionate effect on the maintenance cost at the plant. In the cases examined, each percent variation in ash content could have a monetary effect of from $0.05 to $0.10 per ton of coal consumed. Similarly, each percent variation in sulfur content could influence maintenance costs from $0.30 to $0.50 per ton of coal. Since these values are based on preliminary analysis of limited data, they must be approached with caution and not removed from the context in which they are presented. However, if borne out by further study, the potential magnitude of such savings may be sufficient to justify the acquisition of superior coal supplies, either by changing the source and/or using preparation to obtain a lower ash and sulfur fuel.

  2. [Profiles of the utilization of 20 amino acids as the only source of nitrogen and carbon in bacteria of the genera Klebsiella, Enterobacter, Serratia, Escherichia].

    Science.gov (United States)

    Sivolodskiĭ, E P

    2005-01-01

    The profiles of the utilization of 20 protein amino acids in 118 Klebsiella pneumoniae sub- sp. pneumoniae, K. oxytoca, K. planticola, K. mobilis, Enterobacter cloacae, Serratia marscescens, S. liquefaciens, Escherichia coli strains isolated from clinical material were studied. The utilization of amino acids was determined on minimal saline agar containing amino acid as the only source of nitrogen and carbon; the results were evaluated after 72-hour incubation at 37 degrees C. 17 profiles of amino-acid utilization were thus determined, most of them genus-specific in enterobacteria: Klebsiella (profiles No. 1--6, 9, 10), Enterobacter (No. 11--13), Serratia (No. 14--16), Escherichia (No. 17). The full coincidence of amino-acid utilization profiles in bacteria of K. mobilis (No. 1, 6) and K. pneumoniae subsp. pneumoniae with out of such profiles in bacteria of the genera Enterobacter, Serratia, Escherichia was established, which confirmed that K. mobilis (formerly Enterobacter aerogenes) belonged to the genus Klebsiella.

  3. Final report on the safety assessment of Malic Acid and Sodium Malate.

    Science.gov (United States)

    Fiume, Z

    2001-01-01

    Malic Acid functions in cosmetic formulations as a pH adjuster, and Sodium Malate functions as a skin conditioning agent-humectant. Malic Acid is reportedly used in almost 50 cosmetic formulations across a range of product types at low concentrations, whereas Sodium Malate is used in only one. As a pH adjuster, Malic Acid is used at low concentrations. One commercial method of preparing Malic Acid is hydration of fumaric acid or maleic acid, and then purified to limit the amount of the starting material present. Because Malic Acid is a component of the Kreb's cycle, another method is fermentation. Malic Acid was relatively nontoxic in acute toxicity studies using animals. In a chronic oral study, feeding Malic Acid to rats resulted only in weight gain changes and changes in feed consumption. Malic Acid did not cause reproductive toxicity in mice, rats, or rabbits. Malic Acid was a moderate to strong skin irritatant in animal tests, and was a strong ocular irritant. Malic Acid was not mutagenic across a range of genotoxicity tests. Malic Acid was irritating in clinical tests, with less irritation seen as pH of the applied material increased. Patients patch tested with Malic Acid, placed on a diet that avoided foods containing Malic or citric acid, and then challenged with a diet high in Malic and citric acid had both immediate urticarial and delayed contact dermatitis reactions. These data were considered sufficient to determine that Malic Acid and Sodium Malate would be safe at the low concentrations at which these ingredients would be used to adjust pH (even though Sodium Malate is not currently used for that purpose). The data, however, were insufficient to determine the safety of these ingredients when used in cosmetics as other than pH adjusters and specifically, the data are insufficient to determine the safety of Sodium Malate when used as a skin conditioning agent-humectant. The types of data required for the Expert Panel to determine the safety of Sodium

  4. Scaleable production and separation of fermentation-derived acetic acid. Final CRADA report.

    Energy Technology Data Exchange (ETDEWEB)

    Snyder, S. W.; Energy Systems

    2010-02-08

    Half of U.S. acetic acid production is used in manufacturing vinyl acetate monomer (VAM) and is economical only in very large production plants. Nearly 80% of the VAM is produced by methanol carbonylation, which requires high temperatures and exotic construction materials and is energy intensive. Fermentation-derived acetic acid production allows for small-scale production at low temperatures, significantly reducing the energy requirement of the process. The goal of the project is to develop a scaleable production and separation process for fermentation-derived acetic acid. Synthesis gas (syngas) will be fermented to acetic acid, and the fermentation broth will be continuously neutralized with ammonia. The acetic acid product will be recovered from the ammonium acid broth using vapor-based membrane separation technology. The process is summarized in Figure 1. The two technical challenges to success are selecting and developing (1) microbial strains that efficiently ferment syngas to acetic acid in high salt environments and (2) membranes that efficiently separate ammonia from the acetic acid/water mixture and are stable at high enough temperature to facilitate high thermal cracking of the ammonium acetate salt. Fermentation - Microbial strains were procured from a variety of public culture collections (Table 1). Strains were incubated and grown in the presence of the ammonium acetate product and the fastest growing cultures were selected and incubated at higher product concentrations. An example of the performance of a selected culture is shown in Figure 2. Separations - Several membranes were considered. Testing was performed on a new product line produced by Sulzer Chemtech (Germany). These are tubular ceramic membranes with weak acid functionality (see Figure 3). The following results were observed: (1) The membranes were relatively fragile in a laboratory setting; (2) Thermally stable {at} 130 C in hot organic acids; (3) Acetic acid rejection > 99%; and (4

  5. Acid deposition: decision framework. Volume 1. Description of conceptual framework and decision-tree models. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Balson, W.E.; Boyd, D.W.; North, D.W.

    1982-08-01

    Acid precipitation and dry deposition of acid materials have emerged as an important environmental issue affecting the electric utility industry. This report presents a framework for the analysis of decisions on acid deposition. The decision framework is intended as a means of summarizing scientific information and uncertainties on the relation between emissions from electric utilities and other sources, acid deposition, and impacts on ecological systems. The methodology for implementing the framework is that of decision analysis, which provides a quantitative means of analyzing decisions under uncertainty. The decisions of interest include reductions in sulfur oxide and other emissions thought to be precursors of acid deposition, mitigation of acid deposition impacts through means such as liming of waterways and soils, and choice of strategies for research. The report first gives an overview of the decision framework and explains the decision analysis methods with a simplified caricature example. The state of scientific information and the modeling assumptions for the framework are then discussed for the three main modules of the framework: emissions and control technologies; long-range transport and chemical conversion in the atmosphere; and ecological impacts. The report then presents two versions of a decision tree model that implements the decision framework. The basic decision tree addresses decisions on emissions control and mitigation in the immediate future and a decade hence, and it includes uncertainties in the long-range transport and ecological impacts. The research emphasis decision tree addresses the effect of research funding on obtaining new information as the basis for future decisions. Illustrative data and calculations using the decision tree models are presented.

  6. Effect of dietary fatty acid composition on substrate utilization and body weight maintenance in humans.

    Science.gov (United States)

    Krishnan, Sridevi; Cooper, Jamie A

    2014-04-01

    Dietary fat content is a primary factor associated with the increase in global obesity rates. There is a delay in achieving fat balance following exposure to a high-fat (HF) diet (≥ 40% of total energy from fat) and fat balance is closely linked to energy balance. Exercise has been shown to improve this rate of adaptation to a HF diet. Recently, however, the role of dietary fatty acid composition on energy and macronutrient balance has come into question. We chose studies that compared monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), and saturated fatty acids (SFA). We have reviewed studies that measured diet-induced thermogenesis (DIT), energy expenditure (EE), or fat oxidation (FOx) in response to a HF meal challenge, or long-term dietary intervention comparing these fatty acids. While single-meal studies show that SFA induce lower DIT and FOx compared to unsaturated fats, the effect of the degree of unsaturation (MUFA vs. PUFA) appears to yet be determined. Long-term dietary interventions also support the notion that unsaturated fats induce greater EE, DIT, and/or FOx versus SFA and that a high MUFA diet induces more weight loss compared to a high SFA diet. Sex and BMI status also affect the metabolic responses to different fatty acids; however, more research in these areas is warranted. SFA are likely more obesigenic than MUFA, and PUFA. The unsaturated fats appear to be more metabolically beneficial, specifically MUFA ≥ PUFA > SFA, as evidenced by the higher DIT and FOx following HF meals or diets.

  7. Laboratory study of acid stimulation of drilling-mud-damaged geothermal-reservoir materials. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1983-05-01

    Presented here are the results of laboratory testing performed to provide site specific information in support of geothermal reservoir acidizing programs. The testing program included laboratory tests performed to determine the effectiveness of acid treatments in restoring permeability of geologic materials infiltrated with hydrothermally altered sepiolite drilling mud. Additionally, autoclave tests were performed to determine the degree of hydrothermal alteration and effects of acid digestion on drilling muds and drill cuttings from two KGRA's. Four laboratory scale permeability/acidizing tests were conducted on specimens prepared from drill cuttings taken from two geothermal formations. Two tests were performed on material from the East Mesa KGRA Well No. 78-30, from a depth of approximately 5500 feet, and two tests were performed on material from the Roosevelt KGRA Well No. 52-21, from depths of approximately 7000 to 7500 feet. Tests were performed at simulated in situ geothermal conditions of temperature and pressure.

  8. Benefit-cost analysis of DOE's Current Federal Program to increase hydrothermal resource utilization. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1981-12-10

    The impact of DOE's Current Federal Program on the commercialization of hydrothermal resources between 1980 and 2000 is analyzed. The hydrothermal resources of the United States and the types of DOE activities used to stimulate the development of these resources for both electric power and direct heat use are described briefly. The No Federal Program and the Current Federal Program are then described in terms of funding levels and the resultant market penetration estimates through 2000. These market penetration estimates are also compared to other geothermal utilization forecasts. The direct benefits of the Current Federal Program are next presented for electric power and direct heat use applications. An analysis of the external impacts associated with the additional hydrothermal resource development resulting from the Current Federal Program is also provided. Included are environmental effects, national security/balance-of-payments improvements, socioeconomic impacts and materials requirements. A summary of the analysis integrating the direct benefits, external impacts and DOE program costs concludes the report.

  9. Low NO{sub x} turbine power generation utilizing low Btu GOB gas. Final report, June--August 1995

    Energy Technology Data Exchange (ETDEWEB)

    Ortiz, I.; Anthony, R.V.; Gabrielson, J.; Glickert, R.

    1995-08-01

    Methane, a potent greenhouse gas, is second only to carbon dioxide as a contributor to potential global warming. Methane liberated by coal mines represents one of the most promising under exploited areas for profitably reducing these methane emissions. Furthermore, there is a need for apparatus and processes that reduce the nitrogen oxide (NO{sub x}) emissions from gas turbines in power generation. Consequently, this project aims to demonstrate a technology which utilizes low grade fuel (CMM) in a combustion air stream to reduce NO{sub x} emissions in the operation of a gas turbine. This technology is superior to other existing technologies because it can directly use the varying methane content gases from various streams of the mining operation. The simplicity of the process makes it useful for both new gas turbines and retrofitting existing gas turbines. This report evaluates the feasibility of using gob gas from the 11,000 acre abandoned Gateway Mine near Waynesburg, Pennsylvania as a fuel source for power generation applying low NO{sub x} gas turbine technology at a site which is currently capable of producing low grade GOB gas ({approx_equal} 600 BTU) from abandoned GOB areas.

  10. City of El Centro geothermal energy utility core field experiment. Final report, February 16, 1979-November 30, 1984

    Energy Technology Data Exchange (ETDEWEB)

    Province, S.G.; Sherwood, P.B.

    1984-11-01

    The City of El Centro was awarded a contract in late 1978 to cost share the development of a low to moderate temperature geothermal resource in the City. The resource would be utilized to heat, cool and provide hot water to the nearby Community Center. In December 1981, Thermal 1 (injector) was drilled to 3970 feet. In January 1982, Thermal 2 (producer) was drilled to 8510 feet. Before testing began, fill migrated into both wells. Both wells were cleaned out. A pump was installed in the producer, but migration of fill again into the injector precluded injection of produced fluid. A short term production test was undertaken and results analyzed. Based upon the analysis, DOE decided that the well was not useful for commercial production due to a low flow rate, the potential problems of continued sanding and gasing, and the requirement to lower the pump setting depth and the associated costs of pumping. There was no commercial user found to take over the wells. Therefore, the wells were plugged and abandoned. The site was restored to its original condition.

  11. Volatile fatty acid formation and utilization in anaerobic sulphidogenic batch reactors

    CSIR Research Space (South Africa)

    Greben, HA

    2006-05-01

    Full Text Available feed are converted into microbial cells and into acetic, propionic and butyric acids, which acids are food for the ruminant. The rumen is inhabited with billions of bacteria and protozoa which can efficiently execute the anaerobic degradation..., K.J. 2000). Relative contribution of bacteria, protozoa and fungi to in vitro degradation of orchard grass cell walls and their interaction. App. Environ Micr. 66 (9): 3807-3813. 8 Sonakya, V., Raizada, N., Dalhoff, R and Wilderer, P.A. (2003...

  12. Cyanuric Acid-Based Organocatalyst for Utilization of Carbon Dioxide at Atmospheric Pressure.

    Science.gov (United States)

    Yu, Bing; Kim, Daeun; Kim, Seoksun; Hong, Soon Hyeok

    2017-03-22

    A organocatalytic system based on economical and readily available cyanuric acid has been developed for the synthesis of 2-oxazolidinones and quinazoline-2,4(1H,3H)-diones from propargylamines and 2-aminobenzonitriles under atmospheric pressure carbon dioxide. Notably, a low concentration of carbon dioxide in air was directly converted into 2-oxazolidinone in excellent yields without an external base. Through mechanistic investigation by in situ FTIR spectroscopy, cyanuric acid was demonstrated to be an efficient catalyst for carbon dioxide fixation. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Economic comparison of hydrogen production using sulfuric acid electrolysis and sulfur cycle water decomposition. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Farbman, G.H.; Krasicki, B.R.; Hardman, C.C.; Lin, S.S.; Parker, G.H.

    1978-06-01

    An evaluation of the relative economics of hydrogen production using two advanced techniques was performed. The hydrogen production systems considered were the Westinghouse Sulfur Cycle Water Decomposition System and a water electrolysis system employing a sulfuric acid electrolyte. The former is a hybrid system in which hydrogen is produced in an electrolyzer which uses sulfur dioxide to depolarize the anode. The electrolyte is sulfuric acid. Development and demonstration efforts have shown that extremely low cell voltages can be achieved. The second system uses a similar sulfuric acid electrolyte technology in water electrolysis cells. The comparative technoeconomics of hydrogen produced by the hybrid Sulfur Cycle and by water electrolysis using a sulfuric acid electrolyte were determined by assessing the performance and economics of 380 million SCFD plants, each energized by a very high temperature nuclear reactor (VHTR). The evaluation concluded that the overall efficiencies of hydrogen production, for operating parameters that appear reasonable for both systems, are approximately 41% for the sulfuric acid electrolysis and 47% for the hybrid Sulfur Cycle. The economic evaluation of hydrogen production, based on a 1976 cost basis and assuming a developed technology for both hydrogen production systems and the VHTRs, indicated that the hybrid Sulfur Cycle could generate hydrogen for a total cost approximately 6 to 7% less than the cost from the sulfuric acid electrolysis plant.

  14. Integrated assessment of acid deposition impacts using reduced-form modeling. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Sinha, R.; Small, M.J.

    1996-05-01

    Emissions of sulfates and other acidic pollutants from anthropogenic sources result in the deposition of these acidic pollutants on the earth`s surface, downwind of the source. These pollutants reach surface waters, including streams and lakes, and acidify them, resulting in a change in the chemical composition of the surface water. Sometimes the water chemistry is sufficiently altered so that the lake can no longer support aquatic life. This document traces the efforts by many researchers to understand and quantify the effect of acid deposition on the water chemistry of populations of lakes, in particular the improvements to the MAGIC (Model of Acidification of Groundwater in Catchments) modeling effort, and describes its reduced-form representation in a decision and uncertainty analysis tool. Previous reduced-form approximations to the MAGIC model are discussed in detail, and their drawbacks are highlighted. An improved reduced-form model for acid neutralizing capacity is presented, which incorporates long-term depletion of the watershed acid neutralization fraction. In addition, improved fish biota models are incorporated in the integrated assessment model, which includes reduced-form models for other physical and chemical processes of acid deposition, as well as the resulting socio-economic and health related effects. The new reduced-form lake chemistry and fish biota models are applied to the Adirondacks region of New York.

  15. Caenorhabditis elegans utilizes dauer pheromone biosynthesis to dispose of toxic peroxisomal fatty acids for cellular homoeostasis

    Science.gov (United States)

    Caenorhabditis elegans secretes a dauer pheromone or daumone composed of ascarylose and a fatty acid side chain, perception of which enables worms to gauge depletion of food or a high worm population density. As a result, worms enter the dauer state, a specific developmental stage capable of surviv...

  16. Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus.

    Science.gov (United States)

    Huang, Chao; Yang, Xiao-Yan; Xiong, Lian; Guo, Hai-Jun; Luo, Jun; Wang, Bo; Zhang, Hai-Rong; Lin, Xiao-Qing; Chen, Xin-De

    2015-02-01

    In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus. After 2 weeks' static fermentation, a BC yield of 4 g/L could be obtained. Both effects of medium composition and fermentation condition on the BC production were evaluated. Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield. Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled. The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value. Moreover, the BC sample had great water-holding capacity. Overall, corncob acid hydrolysate could be one promising substrate for BC production.

  17. L-citrulline-malate influence over branched chain amino acid utilization during exercise.

    Science.gov (United States)

    Sureda, Antoni; Córdova, Alfredo; Ferrer, Miguel D; Pérez, Gerardo; Tur, Josep A; Pons, Antoni

    2010-09-01

    Exhaustive exercise induces disturbances in metabolic homeostasis which can result in amino acid catabolism and limited L-arginine availability. Oral L-citrulline supplementation raises plasma L-arginine concentration and augments NO-dependent signalling. Our aim was to evaluate the effects of diet supplementation with L-citrulline-malate prior to intense exercise on the metabolic handle of plasma amino acids and on the products of metabolism of arginine as creatinine, urea and nitrite and the possible effects on the hormonal levels. Seventeen voluntary male pre-professional cyclists were randomly assigned to one of two groups: control or supplemented (6 g L-citrulline-malate 2 h prior exercise) and participated in a 137-km cycling stage. Blood samples were taken in basal conditions, 15 min after the race and 3 h post race (recovery). Most essential amino acids significantly decreased their plasma concentration as a result of exercise; however, most non-essential amino acids tended to significantly increase their concentration. Citrulline-malate ingestion significantly increased the plasma concentration of citrulline, arginine, ornithine, urea, creatinine and nitrite (p urea.

  18. Recovery of calcium carbonate from steelmaking slag and utilization for acid mine drainage pre-treatment.

    Science.gov (United States)

    Mulopo, J; Mashego, M; Zvimba, J N

    2012-01-01

    The conversion of steelmaking slag (a waste product of the steelmaking process) to calcium carbonate (CaCO(3)) was tested using hydrochloric acid, ammonium hydroxide and carbon dioxide via a pH-swing process. Batch reactors were used to assess the technical feasibility of calcium carbonate recovery and its use for pre-treatment of acid mine drainage (AMD) from coal mines. The effects of key process parameters, such as the amount of acid (HCl/calcium molar ratio), the pH and the CO(2) flow rate were considered. It was observed that calcium extraction from steelmaking slag significantly increased with an increase in the amount of hydrochloric acid. The CO(2) flow rate also had a positive effect on the carbonation reaction rate but did not affect the morphology of the calcium carbonate produced for values less than 2 L/min. The CaCO(3) recovered from the bench scale batch reactor demonstrated effective neutralization ability during AMD pre-treatment compared with the commercial laboratory grade CaCO(3).

  19. Effects of atmospheric variability on energy utilization and conservation. Final report, 1 January 1979-31 December 1979

    Energy Technology Data Exchange (ETDEWEB)

    Reiter, E.R.; Burns, C.C.; Cochrane, H.; Johnson, G.R.; Leong, H.; McKean, J.; Sheaffer, J.D.; Starr, A.M.; Webber, J.

    1980-04-01

    An interdisciplinary approach towards a detailed assessment of energy consumption in urban space-heating and cooling is presented in terms of measurement and modeling results. Modeling efforts concentrated on the city of Minneapolis, MN, using data from the winter seasons 1977/78 and 1978/79. Further developments of a reference model also fall back on data from Cheyenne, WY, and Greeley, CO. Mean absolute daily errors of gas consumption estimated by the physical model applied to Minneapolis are 6.26% when compared to actual energy usage for the period 12/1/77 to 2/28/78. The mean daily absolute errors for the statistical reference model for the same time period were 5.54%. Modeling of the energy consumption required detailed input of meteorological parameters from a special network of stations. As a spin-off an assessment was obtained of the effects of anthropogenic heat on urban heat-island generation under various synoptic conditions. A detailed building census, comprised of 105.722 heated structures, was obtained. A field survey in Greeley indicated that investment returns from insulating houses might not be as high as hoped for; possibly a considerable amount of insulating material is applied wastefully. Misinformation seems to be the primary cause of misguided energy conservation. Progress in conservation could be achieved if utility costs were considered in mortgage-loan applications, together with principal, interests, taxes, and insurance. Detailed energy-consumption modeling would be a premise for such fiscal-management approaches. Another extensive field survey yielded data for a local input-output model applied to the city of Greeley. Economic multipliers for dollars of output, space heating, energy use, and employment were developed and used for growth projections to the year 2003 under varying scenarios.

  20. Does feeding frequency affect utilization of added amino acids in Nile tilapia?

    OpenAIRE

    Hoque, Rezaul; Bajgai, Biswas

    2014-01-01

    Nile tilapia (Oreochromis niloticus) is one of the major farmed fish species, with main production in Asia, South and Central America that can tolerate a wide range of environmental stress and easily adapt with low quality of feed ingredients. The aims of the experiments were to determine effects of feeding frequency on utilization of protein and energy in Nile tilapia, to quantify differences in excretion of ammonia and ammonium in Nile tilapia fed the same daily ration, distributed over 2 a...

  1. Influence of protein source on amino acid uptake patterns and protein utilization in rainbow trout Oncorhynchus mykiss

    DEFF Research Database (Denmark)

    Rolland, Marine; Holm, Jørgen; Dalsgaard, Anne Johanne Tang

    Matrixes of different protein sources (fish and plant products) combined with the use of crystalline amino acids allow for formulation of diets that meet fish requirements with little or no effect on protein digestibility and/or feed intake. Despite this, a total or partial replacement of fish meal...... induces reduced growth performances that remain partly unexplained. The aim of the current study was to investigate the effect of exchanging the protein source on protein utilization. Marine (fish meal) and vegetable (pea protein) sources were used with or without supplementation of crystalline amino...

  2. Effects of root-zone acidity on utilization of nitrate and ammonium in tobacco plants

    Science.gov (United States)

    Henry, L. T.; Raper, C. D. Jr; Raper CD, J. r. (Principal Investigator)

    1989-01-01

    Tobacco (Nicotiana tabacum L., cv. 'Coker 319') plants were grown for 28 days in flowing nutrient culture containing either 1.0 mM NO3- or 1.0 mM NH4+ as the nitrogen source in a complete nutrient solution. Acidities of the solutions were controlled at pH 6.0 or 4.0 for each nitrogen source. Plants were sampled at intervals of 6 to 8 days for determination of dry matter and nitrogen accumulation. Specific rates of NO3- or NH4+ uptake (rate of uptake per unit root mass) were calculated from these data. Net photosynthetic rates per unit leaf area were measured on attached leaves by infrared gas analysis. When NO3- [correction of NO-] was the sole nitrogen source, root growth and nitrogen uptake rate were unaffected by pH of the solution, and photosynthetic activity of leaves and accumulation of dry matter and nitrogen in the whole plant were similar. When NH4+ was the nitrogen source, photosynthetic rate of leaves and accumulation of dry matter and nitrogen in the whole plant were not statistically different from NO3(-) -fed plants when acidity of the solution was controlled at pH 6.0. When acidity for NH4(+) -fed plants was increased to pH 4.0, however, specific rate of NH4+ uptake decreased by about 50% within the first 6 days of treatment. The effect of acidity on root function was associated with a decreased rate of accumulation of nitrogen in shoots that was accompanied by a rapid cessation of leaf development between days 6 and 13. The decline in leaf growth rate of NH4(+) -fed plants at pH 4.0 was followed by reductions in photosynthetic rate per unit leaf area. These responses of NH4(+) -fed plants to increased root-zone acidity are characteristic of the sequence of responses that occur during onset of nitrogen stress.

  3. Determination of true digestible amino acids of feedstuffs utilizing cecectomized roosters

    Directory of Open Access Journals (Sweden)

    Eliane Aparecida da Silva

    2012-09-01

    Full Text Available The objective of this study was to estimate the true digestibility coefficients of amino acids and digestible amino acid values of some poultry feedstuffs. The feedstuffs were: babassu meal, sunflower meal, corn gluten meal, babassu starchy meal, meat and bone meal, common beans, pearl millet and residues of cookies, pasta and bread. The precise feeding method of Sibbald was used with adult cecectomized Leghorn roosters distributed in a completely randomized design, consisting of ten treatments and six replications with a rooster in each. The treatments were represented by the feedstuffs evaluated. The roosters were kept in a period of fasting for 36 hours and then fed 30 grams of feed. Samples were collected during 56 hours. Simultaneously, six roosters were kept fasting to make corrections to the metabolic and endogenous losses of amino acids. At the end of collections, the excreta obtained were weighed, freeze-dried and subsequently processed, so laboratory analyses were carried out and the coefficients of true digestibility of amino acids were determined. The mean values of the coefficients of true digestibility of essential and non-essential amino acids in percentage were respectively: 0.702 and 0.652 for the babassu meal; 0.852 and 0.786 for the sunflower meal; 0.928 and 0.887 for the corn gluten meal; 0.797 and 0.720 for the meat and bone meal; 0.364 and 0.339 for ground raw beans; 0.924 and 0.837 for ground pearl millet; 0.839 and 0.810 for cookie residue; 0.929 and 0.914 for pasta residue; and 0.904 and 0.899 for bread residue.

  4. Bacterial utilization of L-sugars and D-amino acids

    Science.gov (United States)

    Pikuta, Elena V.; Hoover, Richard B.; Klyce, Brig; Davies, Paul C. W.; Davies, Pauline

    2006-08-01

    The fact that organotrophic organisms on Earth use L-amino acids and D-sugars as an energy source is recognized as one of the universal features of life. The chirality of organic molecules with asymmetric location of group-radicals was described a relatively long time ago. Louis Pasteur observed that abiotic (chemical) processes produced mixtures with equal numbers (racemic) of the two forms but that living organisms possessed a molecular asymmetry that included only one of the enantiomers (homochirality). He speculated that the origin of the asymmetry of chiral biomolecules might hold the key to the nature of life. All of the amino acids in proteins (except for Glycine which is symmetrical) exhibit the same absolute steric configuration as L-glyceraldehyde. D-amino acids are never found in proteins, although they do exist in nature and are often found in polypeptide antibiotics. Constitutional sugars of cells, opposite to the amino acids, are the D-enantiomers, and the appearance of L-sugars in Nature is extremely rare. Notwithstanding this fact, the metabolism of some bacteria does have the capability to use amino acids and sugars with alternative chirality. This property may be caused by the function of specific enzymes belonging to the class of isomerases (racemases, epimerases, isomerases, tautomerases). In our laboratory, we have investigated several anaerobic bacterial strains, and have found that some of these bacteria are capable of using D-amino acids and L-sugars. Strain BK1 is capable of growth on D-arginine, but its growth characteristics on L-arginine are approximately twice as high. Another alkaliphilic strain SCA T (= ATCC BAA-1084 T = JCM 12857 T = DSM 17722 T = CIP 107910 T) was found to be capable of growth on L-ribose and L-arabinose. It is interesting that this strain was incapable of growth on D-arabinose, which suggests the involvement of some alternative mechanism of enzyme activity. In this paper, we describe the preliminary results of

  5. Acid mine drainage potential of raw, retorted, and combusted Eastern oil shale: Final report

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, P.J.; Yelton, J.L.; Reddy, K.J.

    1987-09-01

    In order to manage the oxidation of pyritic materials effectively, it is necessary to understand the chemistry of both the waste and its disposal environment. The objective of this two-year study was to characterize the acid production of Eastern oil shale waste products as a function of process conditions, waste properties, and disposal practice. Two Eastern oil shales were selected, a high pyrite shale (unweathered 4.6% pyrite) and a low pyrite shale (weathered 1.5% pyrite). Each shale was retorted and combusted to produce waste products representative of potential mining and energy conversion processes. By using the standard EPA leaching tests (TCLP), each waste was characterized by determining (1) mineralogy, (2) trace element residency, and (3) acid-base account. Characterizing the acid producing potential of each waste and potential trace element hazards was completed with laboratory weathering studies. 32 refs., 21 figs., 12 tabs.

  6. Utilization of DNA as functional materials: preparation of filters containing DNA insolubilized with alginic acid gel.

    Science.gov (United States)

    Iwata, K; Sawadaishi, T; Nishimura, S I; Tokura, S; Nishi, N

    1996-02-01

    Thin films composed of DNA and alginic acid were prepared by casting their mixed solution on glass plate followed by coagulation with aqueous solution of calcium chloride. DNA could be conveniently insolubilized by this method. DNA in the films adsorbed intercalating materials, such as ethidium bromide. This phenomenon was successfully applied to the preparation of filters for the selective removal or accumulation of harmful intercalating pollutants.

  7. The absence of protein--sparing effects utilizing crystalline amino acids in stressed patients.

    Science.gov (United States)

    Ching, N; Mills, C J; Grossi, C; Angers, J W; Jham, G; Zurawinsky, H; Nealon, T F

    1979-11-01

    The protein-sparing effects of the peripheral infusion of crystalline amino acids (PAA) was studied metabolically in selected surgical patients subjected to various degrees of stress. Twenty-one patients (sixteen cancer patients receiving chemotherapy and/or radiotherapy, three with major abdominal traumatic injuries and four with paralytic ileus) were infused with 2 1/24 hours of a solution of 4.2% Travasol amino acids with only 5% glucose as a source of nonprotein calories. One-half of the cancer patients were also allowed ad libitum oral intake of a regular hospital diet or Vivonex-HN. The nutritional status was evaluated by measuring changes in body weight, serum albumin levels and nitrogen balance. Body weight decreased in only the trauma patients. When these solutions were the sole source of nutrients all patients were in negative nitrogen balance and had significant decreases in their serum albumin levels. Serum albumin levels were preserved only when extra sources of calories were provided. The infusion of the crystalline amino acids without adequate levels of nonprotein energy did not conserve protein in these stressed patients.

  8. Final Report on Utilization of TRU TRISO Fuel as Applied to HTR Systems Part I: Pebble Bed Reactors

    Energy Technology Data Exchange (ETDEWEB)

    Brian Boer; Abderrafi M. Ougouag

    2011-03-01

    significant failure is to be expected for the reference fuel particle during normal operation. It was found, however, that the sensitivity of the coating stress to the CO production in the kernel was large. The CO production is expected to be higher in DB fuel than in UO2 fuel, but its exact level has a high uncertainty. Furthermore, in the fuel performance analysis transient conditions were not yet taken into account. The effort of this task in FY 2010 has focused on the optimization of the core to maximize the pebble discharge burnup level, while retaining its inherent safety characteristics. Using generic pebble bed reactor cores, this task will perform physics calculations to evaluate the capabilities of the pebble bed reactor to perform utilization and destruction of LWR used-fuel transuranics. The task will use established benchmarked models, and will introduce modeling advancements appropriate to the nature of the fuel considered (high transuranic [TRU] content and high burn-up). Accomplishments of this work include: •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel. •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel and Uranium. •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel and Modified Open Cycle Components. •Core analysis of a HTR-MODULE design loaded with Deep-Burn fuel and Americium targets.

  9. The utility of gadoteric acid in contrast-enhanced MRI: a review

    Directory of Open Access Journals (Sweden)

    Tartaro A

    2015-02-01

    Full Text Available Armando Tartaro, Marica Tina Maccarone Department of Neuroscience, Imaging and Clinical Sciences, and Institute for Advanced Biomedical Technologies (ITAB, “G d’Annunzio” University, Chieti-Pescara, Italy Abstract: Gadoteric acid (Dotarem® is a macrocyclic, paramagnetic, gadolinium-based contrast agent. It is used in the magnetic resonance imaging (MRI of the brain, spine, and associated tissues. Particularly, it is able to detect and visualize areas with disruption of the blood–brain barrier and/or abnormal vascularity. Gadoteric acid has been also approved for MR angiography of supraaortic vessels, cardiac MR (to detect myocardial infarctions, as well as whole-body MRI including abdominal, renal, pelvic, breast, and osteoarticular diseases. Cyclic chelates are more stable compared to linear chelates, and ionic chelates are more stable compared to nonionic chelates. Linear chelates have a greater likelihood of releasing free Gd3+ compared to cyclic chelates. Non-ionic chelates are more likely, compared to ionic chelates, to release Gd3+ from their chelates. Gadoteric acid is a cyclic ionic chelate and has the greatest kinetic stability among gadolinium-based contrast agents. In patients with chronic reduced kidney function, the use of gadolinium-based contrast agents leads to acute kidney injury and dialysis. The risk of acute kidney injury may increase with increasing dose of the contrast agents. Therefore, it is recommended to administer the lowest dose necessary for adequate imaging. The dose reduction allows protection the patients form potential risk of nephrogenic systemic fibrosis, a systemic reaction that is probably due to unbound Gd3+ ions deposited in body tissues. The dose of gadoteric acid should not exceed 0.1 mmol/kg body weight. More than one dose should not be used during a scan. Because of the lack of information on repeated administration, Dotarem® injections should not be repeated unless the interval between

  10. An ortholog of farA of Aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast Yarrowia lipolytica

    Energy Technology Data Exchange (ETDEWEB)

    Poopanitpan, Napapol; Kobayashi, Satoshi; Fukuda, Ryouichi; Horiuchi, Hiroyuki [Department of Biotechnology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657 (Japan); Ohta, Akinori, E-mail: aaohta@mail.ecc.u-tokyo.ac.jp [Department of Biotechnology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657 (Japan)

    2010-11-26

    Research highlights: {yields} POR1 is a Yarrowia lipolytica ortholog of farA involved in fatty acid response in A. nidulans. {yields} Deletion of POR1 caused growth defects on fatty acids. {yields} {Delta}por1 strain exhibited defects in the induction of genes involved in fatty acid utilization. -- Abstract: The yeast Yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. To identify a gene(s) regulating fatty acid utilization in Y. lipolytica, we first studied homologous genes to OAF1 and PIP2 of Saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. We next characterized a Y. lipolytica gene, POR1 (primary oleate regulator 1), an ortholog of farA encoding a transcriptional activator that regulates fatty acid utilization in Aspergillus nidulans. The deletion mutant of POR1 was defective in the growth on various fatty acids, but not on glucose, glycerol, or n-hexadecane. It exhibited slight defect on n-decane. The transcriptional induction of genes involved in {beta}-oxidation and peroxisome proliferation by oleate was distinctly diminished in the {Delta}por1 strains. These data suggest that POR1 encodes a transcriptional activator widely regulating fatty acid metabolism in Y. lipolytica.

  11. Stability of antimicrobial activity of peracetic acid solutions used in the final disinfection process

    Directory of Open Access Journals (Sweden)

    Solange Alves da Silva COSTA

    2015-01-01

    Full Text Available The instruments and materials used in health establishments are frequently exposed to microorganism contamination, and chemical products are used before sterilization to reduce occupational infection. We evaluated the antimicrobial effectiveness, physical stability, and corrosiveness of two commercial formulations of peracetic acid on experimentally contaminated specimens. Stainless steel specimens were contaminated with Staphylococcus aureus, Escherichia coli, Candida albicans, blood, and saliva and then immersed in a ready peracetic acid solution: 2% Sekusept Aktiv (SA or 0.25% Proxitane Alpha (PA, for different times. Then, washes of these instruments were plated in culture medium and colony-forming units counted. This procedure was repeated six times per day over 24 non-consecutive days. The corrosion capacity was assessed with the mass loss test, and the concentration of peracetic acid and pH of the solutions were measured with indicator tapes. Both SA and PA significantly eliminated microorganisms; however, the SA solution was stable for only 4 days, whereas PA remained stable throughout the experiment. The concentration of peracetic acid in the SA solutions decreased over time until the chemical was undetectable, although the pH remained at 5. The PA solution had a concentration of 500-400 mg/L and a pH of 2-3. Neither formulation induced corrosion and both reduced the number of microorganisms (p = 0.0001. However, the differences observed in the performance of each product highlight the necessity of establishing a protocol for optimizing the use of each one.

  12. Stability of antimicrobial activity of peracetic acid solutions used in the final disinfection process.

    Science.gov (United States)

    Costa, Solange Alves da Silva; Paula, Olívia Ferreira Pereira de; Silva, Célia Regina Gonçalves E; Leão, Mariella Vieira Pereira; Santos, Silvana Soléo Ferreira dos

    2015-01-01

    The instruments and materials used in health establishments are frequently exposed to microorganism contamination, and chemical products are used before sterilization to reduce occupational infection. We evaluated the antimicrobial effectiveness, physical stability, and corrosiveness of two commercial formulations of peracetic acid on experimentally contaminated specimens. Stainless steel specimens were contaminated with Staphylococcus aureus, Escherichia coli, Candida albicans, blood, and saliva and then immersed in a ready peracetic acid solution: 2% Sekusept Aktiv (SA) or 0.25% Proxitane Alpha (PA), for different times. Then, washes of these instruments were plated in culture medium and colony-forming units counted. This procedure was repeated six times per day over 24 non-consecutive days. The corrosion capacity was assessed with the mass loss test, and the concentration of peracetic acid and pH of the solutions were measured with indicator tapes. Both SA and PA significantly eliminated microorganisms; however, the SA solution was stable for only 4 days, whereas PA remained stable throughout the experiment. The concentration of peracetic acid in the SA solutions decreased over time until the chemical was undetectable, although the pH remained at 5. The PA solution had a concentration of 500-400 mg/L and a pH of 2-3. Neither formulation induced corrosion and both reduced the number of microorganisms (p = 0.0001). However, the differences observed in the performance of each product highlight the necessity of establishing a protocol for optimizing the use of each one.

  13. Case Studies in Systems Chemistry. Final Report. [Includes Complete Case Study, Carboxylic Acid Equilibria

    Science.gov (United States)

    Fleck, George

    This publication was produced as a teaching tool for college chemistry. The book is a text for a computer-based unit on the chemistry of acid-base titrations, and is designed for use with FORTRAN or BASIC computer systems, and with a programmable electronic calculator, in a variety of educational settings. The text attempts to present computer…

  14. Medical Devices; Immunology and Microbiology Devices; Classification of Trichomonas Vaginalis Nucleic Acid Assay. Final order.

    Science.gov (United States)

    2015-08-04

    The Food and Drug Administration (FDA) is classifying a Trichomonas vaginalis nucleic acid assay into class II (special controls). The Agency is classifying the device into class II (special controls) in order to provide a reasonable assurance of safety and effectiveness of the device.

  15. The utility of omega-3 fatty acids in epilepsy: more than just a farmed tilapia!

    Directory of Open Access Journals (Sweden)

    Vera C. Terra

    2011-02-01

    Full Text Available The epilepsies are one of the most common serious brain disorders and 20 to 30% of people developing epilepsy continue to have seizures and are refractory to treatment with the currently available therapies. Approximately one in a 1000 patients with chronic epilepsy will die suddenly, unexpectedly, and without explanation, even with post-mortem examination and this phenomenon is called sudden unexplained death in epilepsy (SUDEP. Understanding the mechanisms underlying SUDEP may lead to the identification of previously unrecognized risk factors that are more amenable to correction. We discuss here the possible implications of omega-3 fatty acids consumption on SUDEP prevention.

  16. Acid-Base Behavior in Hydrothermal Processing of Wastes - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Johnston, K.; Rossky, P.

    2000-12-01

    A major obstacle to development of hydrothermal oxidation technology has been a lack of scientific knowledge of chemistry in hydrothermal solution above 350 C, particularly acid-base behavior, and transport phenomena, which is needed to understand corrosion, metal-ion complexation, and salt precipitation and recovery. Our objective has been to provide this knowledge with in situ UV-visible spectroscopic measurements and fully molecular computer simulation. Our recent development of relatively stable organic UV-visible pH indicators for supercritical water oxidation offers the opportunity to characterize buffers and to monitor acid-base titrations. These results have important implications for understanding reaction pathways and yields for decomposition of wastes in supercritical water.

  17. Food Additives Permitted for Direct Addition to Food for Human Consumption; Folic Acid. Final rule.

    Science.gov (United States)

    2016-04-15

    The Food and Drug Administration (FDA or we) is amending the food additive regulations to provide for the safe use of folic acid in corn masa flour. We are taking this action in response to a food additive petition filed jointly by Gruma Corporation, Spina Bifida Association, March of Dimes Foundation, American Academy of Pediatrics, Royal DSM N.V., and National Council of La Raza.

  18. Microbial utilization of the industrial wastewater pollutants 2-ethylhexylthioglycolic acid and iso-octylthioglycolic acid by aerobic gram-negative bacteria.

    Science.gov (United States)

    Toups, Mario; Wübbeler, Jan Hendrik; Steinbüchel, Alexander

    2010-04-01

    Industrial wastewater from the production of sulfur containing esters and the resulting products of this synthesis, 2-ethylhexylthioglycolic acid (EHTG) and iso-octylthioglycolic acid (IOTG), were deployed in this study to enrich novel bacterial strains, since no wastewater and EHTG or IOTG degrading microorganisms were hitherto described or available. In addition, nothing is known about the biodegradation of these thiochemicals. The effect of this specific wastewater on the growth behaviour of microorganisms was investigated using three well-known Gram-negative bacteria (Escherichia coli, Pseudomonas putida, and Ralstonia eutropha). Concentrations of 5% (v/v) wastewater in complex media completely inhibited growth of these three bacterial strains. Six bacterial strains were successfully isolated, characterized and identified by sequencing their 16S rRNA genes. Two isolates referred to as Achromobacter sp. strain MT-E3 and Pseudomonas sp. strain MT-I1 used EHTG or IOTG, respectively, as well as the wastewater as sole source of carbon and energy for weak growth. More notably, both isolates removed these sulfur containing esters in remarkable amounts from the cultures supernatant. One further isolate was referred to as Klebsiella sp. strain 58 and exhibited an unusual high tolerance against the wastewater's toxicity without utilizing the contaminative compounds. If cultivated with gluconic acid as additional carbon source, the strain grew even in presence of more than 40% (v/v) wastewater. Three other isolates belonging to the genera Bordetella and Pseudomonas tolerated these organic sulfur compounds but showed no degradation abilities.

  19. Improved drug targeting of cancer cells by utilizing actively targetable folic acid-conjugated albumin nanospheres.

    Science.gov (United States)

    Shen, Zheyu; Li, Yan; Kohama, Kazuhiro; Oneill, Brian; Bi, Jingxiu

    2011-01-01

    Folic acid-conjugated albumin nanospheres (FA-AN) have been developed to provide an actively targetable drug delivery system for improved drug targeting of cancer cells with reduced side effects. The nanospheres were prepared by conjugating folic acid onto the surface of albumin nanospheres using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) as a catalyst. To test the efficacy of these nanospheres as a potential delivery platform, doxorubicin-loaded albumin nanospheres (DOX-AN) and doxorubicin-loaded FA-AN (FA-DOX-AN) were prepared by entrapping DOX (an anthracycline, antibiotic drug widely used in cancer chemotherapy that works by intercalating DNA) into AN and FA-AN nanoparticles. Cell uptake of the DOX was then measured. The results show that FA-AN was incorporated into HeLa cells (tumor cells) only after 2.0h incubation, whereas HeLa cells failed to incorporate albumin nanospheres without conjugated folic acid after 4.0h incubation. When HeLa cells were treated with the DOX-AN, FA-DOX-AN nanoparticles or free DOX, cell viability decreased with increasing culture time (i.e. cell death increases with time) over a 70h period. Cell viability was always the lowest for free DOX followed by FA-DOX-AN4 and then DOX-AN. In a second set of experiments, HeLa cells washed to remove excess DOX after an initial incubation for 2h were incubated for 70h. The corresponding cell viability was slightly higher when the cells were treated with FA-DOX-AN or free DOX whilst cells treated with DOX-AN nanoparticles remained viable. The above experiments were repeated for non-cancerous, aortic smooth muscle cells (AoSMC). As expected, cell viability of the HeLa cells (with FA receptor alpha, FRα) and AoSMC cells (without FRα) decreased rapidly with time in the presence of free DOX, but treatment with FA-DOX-AN resulted in selective killing of the tumor cells. These results indicated that FA-AN may be used as a promising actively targetable drug delivery system to improve drug

  20. Utilization of Atikokan coal fly ash in acid rock drainage control from Musselwhite Mine tailings

    Energy Technology Data Exchange (ETDEWEB)

    Wang, H.L.; Shang, J.Q. [Western Ontario Univ., London, ON (Canada). Dept. of Civil and Environmental Engineering; Kovac, V. [Ontario Power Generation Inc., Toronto, ON (Canada). By-products, Fuel Division; Ho, K.S. [Trow Consulting Engineers, Brampton, ON (Canada)

    2006-03-15

    Acid rock drainage (ARD) is the greatest environmental liability facing the mining industry. Mines produce acidic effluents that are generated from the chemical reaction of sulphide containing minerals and atmospheric oxygen. The effluents have a pH value as low as 2 to 4 and their movement is accompanied by heavy metals which damage the ecosystem. This paper described some of the ARD-preventing technologies that are under investigation. In particular, it examined the feasibility of using Atikokan coal fly ash (AFA) as a buffering material to control and mitigate the generation of ARD from reactive Musselwhite Mine gold mine tailings. Coal fly ash is the residue resulting from the combustion of coal at electric generating plants. It consists of organic and inorganic matter, including silica, alumina, iron and calcium oxide with various amounts of carbon. More than 40,000 tons of fly ash is generated each year from the Atikokan Generating Station located 190 km west of the mine, of which 80 per cent is used for concrete manufacturing. In this study, experiments were conducted to determine the physical, chemical and mineralogical properties of both the fly ash residue and mine tailings. Six kinetic column permeation tests were then performed to monitor the leaching properties of the fly ash and the coal fly ash-mine tailings mixtures to determine the hydraulic conductivities resulting from pozzolanic reactions. The potential impacts of the disposal of AFA and mine tailings were also assessed. The study showed that the hydraulic conductivities of high-calcium AFA and the ash-tailings mixtures were greatly reduced upon contact with ARD. The pH of the pore fluid increased from acidic to alkaline. The concentration of regulated elements in the leachate from the ash-tailings mixtures were also below the limits set by the Ontario Ministry of Environment. The results indicate that AFA could mitigate the generation of ARD from reactive Musselwhite Mine gold mine tailings. 1

  1. The influence of alternative pathways of respiration that utilize branched-chain amino acids following water shortage in Arabidopsis.

    Science.gov (United States)

    Pires, Marcel V; Pereira Júnior, Adilson A; Medeiros, David B; Daloso, Danilo M; Pham, Phuong Anh; Barros, Kallyne A; Engqvist, Martin K M; Florian, Alexandra; Krahnert, Ina; Maurino, Veronica G; Araújo, Wagner L; Fernie, Alisdair R

    2016-06-01

    During dark-induced senescence isovaleryl-CoA dehydrogenase (IVDH) and D-2-hydroxyglutarate dehydrogenase (D-2HGDH) act as alternate electron donors to the ubiquinol pool via the electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) pathway. However, the role of this pathway in response to other stresses still remains unclear. Here, we demonstrated that this alternative pathway is associated with tolerance to drought in Arabidopsis. In comparison with wild type (WT) and lines overexpressing D-2GHDH, loss-of-function etfqo-1, d2hgdh-2 and ivdh-1 mutants displayed compromised respiration rates and were more sensitive to drought. Our results demonstrated that an operational ETF/ETFQO pathway is associated with plants' ability to withstand drought and to recover growth once water becomes replete. Drought-induced metabolic reprogramming resulted in an increase in tricarboxylic acid (TCA) cycle intermediates and total amino acid levels, as well as decreases in protein, starch and nitrate contents. The enhanced levels of the branched-chain amino acids in loss-of-function mutants appear to be related to their increased utilization as substrates for the TCA cycle under water stress. Our results thus show that mitochondrial metabolism is highly active during drought stress responses and provide support for a role of alternative respiratory pathways within this response.

  2. Utilization of Vinegar for Isolation of Cellulose Producing Acetic Acid Bacteria

    Science.gov (United States)

    Aydin, Y. Andelib; Aksoy, Nuran Deveci

    2010-06-01

    Wastes of traditionally fermented Turkish vinegar were used in the isolation of cellulose producing acetic acid bacteria. Waste material was pre-enriched in Hestrin-Schramm medium and microorganisms were isolated by plating dilution series on HS agar plates The isolated strains were subjected to elaborate biochemical and physiological tests for identification. Test results were compared to those of reference strains Gluconacetobacter xylinus DSM 46604, Gluconacetobacter hansenii DSM 5602 and Gluconacetobacter liquefaciens DSM 5603. Seventeen strains, out of which only three were found to secrete the exopolysaccharide cellulose. The highest cellulose yield was recorded as 0.263±0.02 g cellulose L-1 for the strain AS14 which resembled Gluconacetobacter hansenii in terms of biochemical tests.

  3. Utilization of 5-aminolevulinic acid in the photodynamic therapy of tumors: biochemical and photobiological aspects

    Science.gov (United States)

    Pottier, Roy H.; Kennedy, James C.

    1994-03-01

    Inherent in both plants and animals is the natural porphyrin, Protoporphyrin IX (Pp). Although Pp does not appear to have any intrinsic biological activity, it is a potent natural photosensitizer. When activated with ultraviolet or visible light, this photosensitizer can induce significant photodynamic effects on tissues, cells, subcellular elements, and macromolecules via the production of singlet oxygen. The biosynthesis of endogenous Pp is under strict enzymatic control. It is possible to bypass a rate controlling step and induce large, transient concentrations of Pp by the addition of exogenous 5-aminolevulinic acid (ALA). ALA may be administered systemically or topically. Much larger amounts of Pp are produced in certain types of tumor tissue than in adjacent normal tissue. Topically applied ALA can be used to treat a variety of skin lesions, including actinic keratosis, basal cell carcinomas and psoriasis.

  4. Evaluation of Gas-Cooled Pressurized Phosphoric Acid Fuel Cells for Electric Utility Power Generation

    Science.gov (United States)

    Faroque, M.

    1983-01-01

    Gas cooling is a more reliable, less expensive and a more simple alternative to conventional liquid cooling for heat removal from the phosphoric acid fuel cell (PAFC). The feasibility of gas-cooling was already demonstrated in atmospheric pressure stacks. Theoretical and experimental investigations of gas-cooling for pressurized PAFC are presented. Two approaches to gas cooling, Distributed Gas-Cooling (DIGAS) and Separated Gas-Cooling (SGC) were considered, and a theoretical comparison on the basis of cell performance indicated SGC to be superior to DIGAS. The feasibility of SGC was experimentally demonstrated by operating a 45-cell stack for 700 hours at pressure, and determining thermal response and the effect of other related parameters.

  5. Final Project Report for project titled "Fluoroalkylphosphonic-acid-based proton conductors"

    Energy Technology Data Exchange (ETDEWEB)

    Stephen Creager

    2011-12-08

    The overall objective of this research was to create new proton-conducting polymer electrolytes for use in energy conversion devices including hydrogen fuel cells that could operate at high temperatures (95-130 C) and under low relative humidity (< 50% RH) conditions. The new polymers were based on the fluoroalkylphosphonic and phosphinic acid (FPA) groups (see illustration below) which offer prospects for rapid proton transport by a proton-hopping mechanism similar to that which operates in phosphoric acid, a well-known proton-transporting electrolyte that is used in a class of hydrogen fuel cells that work well under the conditions noted above and are already commercially successful. The two specific project objectives were as follows: (1) synthesize and characterize new proton-conducting electrolytes based on the fluoroalkylphosphonic and phosphinic acid (FPA) functional groups; and (2) create and apply new computer models to study protonic conduction in FPA-based electrolytes. The project was successful in creating the desired polymer electrolytes and also a series of molecular model compounds which were used to study proton transport in FPA electrolytes in general. Computer models were created to study both structure and proton-transport dynamics in the electrolytes, particularly the molecular model compounds. Rapid proton transport by a hopping mechanism was found in many of the model compounds and correlations with transport rates with molecular structure were identified. Several polymeric analogs of FPA model compounds were prepared and studied, however FPA-based polymeric materials having very high protonic conductivities under either wet or dry conditions were not obtained. Several possible reasons for the failure of polymeric materials to exhibit the expected high protonic conductivities were identified, including a failure of the polymers to adopt the phase-separated secondary structure/morphology necessary for high proton conductivity, and an

  6. Ecological balancing of used oil utilization methods. Final report; Oekologische Bilanzierung von Altoel-Verwertungswegen. Oekologischer Vergleich von vier wichtigen Altoelverwertungsverfahren. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Kolshorn, K.U. [ARCADIS Trischler und Partner GmbH (AT und P), Darmstadt (Germany); Fehrenbach, H. [ifeu-Institut fuer Energie- und Umweltforschung GmbH, Heidelberg (Germany)

    2000-01-01

    In Germany, about 480.000 tons of used oil that are collected each year have to be disposed of and utilized. Four used oil utilization processes (re-processing to base oils; re-processing for fuels; thermal utilization in cement works; pressure gasification for the production of synthesis gas or methanol) were evaluated using an eco-balance. The balance boundary comprises on the one hand the utilization processes and their system-related side-processes (provision of energy, production of auxiliaries, waste disposal, transport) and on the other the processes that are substituted by the products recovered from the utilization processes. The impact assessment covers the indicator list of DIN/NAGUS. Furthermore, the accumulation of pollutants in the products is discussed. On the impact assessment level, no clear ranking of options can be derived since each option has its advantages resp. disadvantages in relation to the other. The reduced impacts of the substituted processes are decisive for the ranking of the option. The final ranking of the options is carried out by the Umweltbundesamt (UBA) based on the interpretation logic of the UBA evaluation method for eco-balances. The present study concludes with the processing and preparation of the balance results for such an interpretation (especially the standardization of the results of the impact assessment based on the total impact assessment based on the total impact in Germany). (orig.) [German] In Deutschland sind jaehrlich ca. 480.000 t Altoel, die im Rahmen der gesetzlich geregelten Altoelsammlung anfallen, zu entsorgen und zu verwerten. Vier Verwertungsverfahren fuer Altoel (Aufarbeitung zu Grundoelen; Aufarbeitung zu Brennstoffen; thermische Nutzung in Zementwerken; Druckvergasung zur Herstellung von Synthesegas bzw. Methanol) wurden anhand einer Oekobilanz bewertet. Der Bilanzraum umfasst einerseits die jeweiligen Verwertungsprozesse incl. der systembedingten Nebenprozesse (Energiebereitstellung

  7. Low-phytic acid corn improves nutrient utilization for growing pigs.

    Science.gov (United States)

    Veum, T L; Ledoux, D R; Raboy, V; Ertl, D S

    2001-11-01

    Thirty-five crossbred barrows averaging 14.5 kg initial BW were used in a 5-wk experiment to compare the P availability and nutritional value of a low-phytate hybrid corn (LPC, 0.26% total P, 0.08% phytic acid P) homozygous for the lpa 1-1 allele with a nearly isogenic normal hybrid corn (NC, 0.25% total P, 0.20% phytic acid P). The pigs were fed individually twice daily in metabolism pens. Three semipurified diets were created in which corn was the only source of phytate. Diet 1 contained 72% NC, 0.15% estimated available P (aP) and 0.55% Ca. Diet 2 contained 72% LPC, 0.24% aP, and 0.55% Ca. The only differences between Diets 1 and 2 were the source of corn and the levels of aP. No inorganic P (iP) was added to these diets in order to measure the animal response to the different levels of aP in the corn hybrids. Diet 3 was NC Diet 1 supplemented with iP to equal the level of aP in LPC Diet 2. Diets 4 and 5 were practical corn-soybean meal diets formulated with each corn to meet all minimum nutrient requirements and contained 0.30% aP and 0.65% Ca. For the semipurified diets, pigs fed LPC Diet 2 had higher (P or = 0.2), indicating an equal nutritional value for both corn hybrids after adjusting for phytate level. The only treatment difference, other than P excretion, between the practical corn diets supplemented with soybean meal was a higher (P < 0.05) bone breaking strength for pigs fed LPC Diet 5 compared with NC Diet 4. The use of LPC in pig diets reduced P excretion in swine waste by 50 and 18.4% in the semipurified and practical diets, respectively, compared with NC. Using our in vitro procedure designed to simulate the digestive system of the pig, the availability of P for pigs was estimated at 56% for LPC and 11% for NC.

  8. Secondary economic impact of acid deposition control legislation in six coal producing states: Final report

    Energy Technology Data Exchange (ETDEWEB)

    Scott, M.J.; Guthrie, S.J.

    1988-12-01

    Among the difficult policy questions on the US environmental agenda is what to do about emissions to the earth's atmosphere of pollutants that may result in ''acid rain''. The Congress has considered several pieces of legislation spelling out potential approaches to the problem and setting goals for emission reduction, mostly emphasizing the control of oxides of sulfur and nitrogen. Significant policy concern is the dollar costs to the nation's economy of achieving the intended effects of the legislation and the potential impacts on economic activity---in particular, losses of both coal mining and secondary service sector employment in states and regions dependent on the mining of high sulfur coal. There are several direct economic effects of regulations such as the acid rain control legislation. One of the more obvious effects was the switching from high sulfur coal to low sulfur coal. This would result in increases in employment and coal business procurements in low sulfur coal mining regions, but also would result in lower employment and lower coal business procurements in high sulfur coal mining areas. The potential negative effects are the immediate policy concern and are the focus of this report. 15 refs., 1 fig., 17 tabs.

  9. Efficacy of different final irrigant activation protocols on smear layer removal by EDTA and citric acid.

    Science.gov (United States)

    Herrera, Daniel R; Santos, Zarina T; Tay, Lidia Y; Silva, Emmanuel J; Loguercio, Alessandro D; Gomes, Brenda P F A

    2013-04-01

    The aim of this study was to evaluate the influence of different activation protocols for chelating agents used after chemo-mechanical preparation (CMP), for smear layer (SL) removal. Forty-five single-rooted human premolars with straight canals and fully formed apex were selected. The specimens were randomly divided into three groups depending on the chelating agent used for smear layer removal: distilled water (DW, control group); 17% ethylenediaminetetraacetic acid (EDTA); and 10% citric acid (CA). Each group was further divided into three subgroups according to the activation protocol used: no-activation (NA), manual dynamic activation (MDA), or sonic activation (SA). After CMP, all specimens were sectioned and processed for observation of the apical thirds by using scanning electron microscopy (SEM). Two calibrated evaluators attributed scores to each specimen. The differences between activation protocols were analyzed with Kruskal-Wallis and Mann-Whitney U tests. Friedman and Wilcoxon signed rank tests were used for comparison between each root canal third. When chelating agents were activated, either by MDA or SA, it was obtained the best cleaning results with no significant difference between EDTA and CA (P > 0.05). Sonic activation showed the best results when root canal thirds were analyzed, in comparison to MDA and NA groups (P benefits smear layer removal from root canals.

  10. A New Process Study on Comprehensive Utilization of NOx from Nitric Acid Plant

    Institute of Scientific and Technical Information of China (English)

    Zhou Hou-zhen; Liang Hua

    2003-01-01

    Based on studies of controlling NOx from nitric acid exhaust gas domestic and abroad, this paper has developed a new technique on removal of NOx and production of a by-product with high added value-- MnO2. Experiment shows, pyrolusite, whose valid composition is MnO2, of absorbing NOx has the following characteristics: quick, low cost, no secondary pollutant. Moreover, exhaust gas can attain the required standards. If the temperature of the pyrolusite serum is 30℃, pH=4, containing 90 g/L MnO2, the absorptivity for 3 000 mg/m3 NOx is 89.2%. Furthermore,few of the impurities in pyrolusite dissolve in the absorption mother solution. The purification cost of product is very low.The purity of end product MnO2 is more than 99. 995% after purifying. Therefore, it is workable for the new technique on economy and technology. There are also obvious environmental benefits and marked economic returns.

  11. A New Process Study on Comprehensive Utilization of NOx from Nitric Acid Plant

    Institute of Scientific and Technical Information of China (English)

    ZhouHou-zhen; LiangHua

    2003-01-01

    Based on studies of controlling NOx from nitric acid exhaust gas domestic and abroad, this paper has developed a new technique on removal of NOx and production of a by-product with high added value MnO2. Experiment shows, pyrolusite, whose valid composition is MnO2, of absorbing NOx has the following characteristics: quick, low cost, no secondary pollutant. Moreover, exhaust gas can attain the required standards. If the temperature of the pyrolusite serum is 30℃, pH=4, containing 90 g/L MnO2, the absorptivity for 3 000 mg/m3 NOx is 89.2%. Furthermore,few of the impurities in pyrolusite dissolve in the absorption mother solution. The purification cost of product is very low.The purity of end product MnO2 is more than 99. 995% after purifying. Therefore, it is workable for the new technique on economy and technology. There are also obvious environmental benefits and marked economic returns.

  12. Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester

    Directory of Open Access Journals (Sweden)

    Hampus Sunner

    2015-09-01

    Full Text Available Research on glucuronoyl esterases (GEs has been hampered by the lack of enzyme assays based on easily obtainable substrates. While benzyl d-glucuronic acid ester (BnGlcA is a commercially available substrate that can be used for GE assays, several considerations regarding substrate instability, limited solubility and low apparent affinities should be made. In this work we discuss the factors that are important when using BnGlcA for assaying GE activity and show how these can be applied when designing BnGlcA-based GE assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery of different GEs through an extensive screening of heterologous Genomic and Metagenomic expression libraries.

  13. Enzymatic synthesis of fatty acid ethyl esters by utilizing camellia oil soapstocks and diethyl carbonate.

    Science.gov (United States)

    Wang, Yingying; Cao, Xuejun

    2011-11-01

    This study was reported on a novel process for fatty acid ethyl esters preparation by transesterification and esterification from renewable low-cost feedstock camellia oil soapstocks and friendly acyl acceptor diethyl carbonate. The main components of product were 83.9% ethyl oleate, 8.9% ethyl palmitate, 4.7% ethyl linoleate and 2.1% ethyl stearate, which could be used as eco-friendly renewable resources or additives of industrial solvent and fossil fuel. The effects of molar ratio of diethyl carbonate to soapstocks oil, lipases, organic solvent, reaction temperature and time were investigated, and process conditions were optimized. The yield was up to 98.4% in solvent-free system with molar ratio of diethyl carbonate to soapstocks oil 3:1 and 5% Novozym 435 (based on the weight of soapstocks oil) at 50 °C and 180 rpm for 24 h. Moreover, there was no obvious loss in the yield after lipases were reused for 10 batches without treatment under optimized conditions. Copyright © 2011 Elsevier Ltd. All rights reserved.

  14. Anti-reflection coatings applied by acid-leaching process. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Pastirik, E.

    1980-09-01

    The Magicote C process developed by S.M. Thompsen was evaluated for use in applying an antireflective coating to the cover plates of solar cell panels. The process uses a fluosilicic acid solution supersaturated with silica at elevated temperature to selectively attack the surface of soda-lime glass cover plates and alter the physical and chemical composition of a thin layer of glass. The altered glass layer constitutes an antireflective coating. The process produces coatings of excellent optical quality which possess outstanding resistance to soiling and staining. The coatings produced are not resistant to mechanical abrasion and are attacked to some extent by glass cleansers. Control of the filming process was found to be difficult.

  15. Final Report Nucleic Acid System - Hybrid PCR and Multiplex Assay Project Phase 2

    Energy Technology Data Exchange (ETDEWEB)

    Koopman, R P; Langlois, R G; Nasarabadi, S; Benett, W J; Colston, B W; Johnson, D C; Brown, S B; Stratton, P L; Milanovich, F P

    2002-04-17

    This report covers phase 2 (year 2) of the Nucleic Acid System--Hybrid PCR and Multiplex Assay project. The objective of the project is to reduce to practice the detection and identification of biological warfare pathogens by the nucleic acid recognition technique of PCR (polymerase chain reaction) in a multiplex mode using flow cytometry. The Hybrid instrument consists of a flow-through PCR module capable of handling a multiplexed PCR assay, a hybridizing module capable of hybridizing multiplexed PCR amplicons and beads, and a flow cytometer module for bead-based identification, all controlled by a single computer. Multiplex immunoassay using bead-based Luminex flow cytometry is available, allowing rapid screening for many agents. PCR is highly specific and complements and verifies immunoassay. It can also be multiplexed and detection provided using the bead-based Luminex flow cytometer. This approach allows full access to the speed and 100-fold multiplex capability of flow cytometry for rapid screening as well as the accuracy and specificity of PCR. This project has two principal activities: (1) Design, build and test a prototype hybrid PCR/flow cytometer with the basic capabilities for rapid, broad spectrum detection and identification, and (2) Develop and evaluate multiplex flow analysis assay protocols and reagents for the simultaneous detection of PCR products. This project requires not only building operationally functional instrumentation but also developing the chemical assays for detection of priority pathogens. This involves development and evaluation of multiplex flow analysis assay protocols and reagents for the simultaneous detection of PCR products.

  16. D-Lactic acid production by Sporolactobacillus inulinus YBS1-5 with simultaneous utilization of cottonseed meal and corncob residue.

    Science.gov (United States)

    Bai, Zhongzhong; Gao, Zhen; Sun, Junfei; Wu, Bin; He, Bingfang

    2016-05-01

    d-Lactic acid, is an important organic acid produced from agro-industrial wastes by Sporolactobacillus inulinus YBS1-5 was investigated to reduce the raw material cost of fermentation. The YBS1-5 strain could produce d-lactic acid by using cottonseed meal as the sole nitrogen source. For efficient utilization, the cottonseed meal was enzymatically hydrolyzed and simultaneously utilized during d-lactic acid fermentation. Corncob residues are rich in cellulose and can be enzymatically hydrolyzed without pretreatment. The hydrolysate of this lignocellulosic waste could be utilized by strain YBS1-5 as a carbon source for d-lactic acid production. Under optimal conditions, a high d-lactic acid concentration (107.2g/L) was obtained in 7-L fed-batch fermenter, with an average productivity of 1.19g/L/h and a yield of 0.85g/g glucose. The optical purity of d-lactic acid in the broth was 99.2%. This study presented a new approach for low-cost production of d-lactic acid for an industrial application.

  17. Involvement of acyl-CoA synthetase genes in n-alkane assimilation and fatty acid utilization in yeast Yarrowia lipolytica.

    Science.gov (United States)

    Tenagy; Park, Jun Seok; Iwama, Ryo; Kobayashi, Satoshi; Ohta, Akinori; Horiuchi, Hiroyuki; Fukuda, Ryouichi

    2015-06-01

    Here, we investigated the roles of YAL1 (FAA1) and FAT1 encoding acyl-CoA synthetases (ACSs) and three additional orthologs of ACS genes FAT2-FAT4 of the yeast Yarrowia lipolytica in the assimilation or utilization of n-alkanes and fatty acids. ACS deletion mutants were generated to characterize their function. The FAT1 deletion mutant exhibited decreased growth on n-alkanes of 10-18 carbons, whereas the FAA1 mutant showed growth reduction on n-alkane of 16 carbons. However, FAT2-FAT4 deletion mutants did not show any growth defects, suggesting that FAT1 and FAA1 are involved in the activation of fatty acids produced during the metabolism of n-alkanes. In contrast, deletions of FAA1 and FAT1-FAT4 conferred no defect in growth on fatty acids. The wild-type strain grew in the presence of cerulenin, an inhibitor of fatty acid synthesis, by utilizing exogenously added fatty acid or fatty acid derived from n-alkane when oleic acid or n-alkane of 18 carbons was supplemented. However, the FAA1 deletion mutant did not grow, indicating a critical role for FAA1 in the utilization of fatty acids. Fluorescent microscopic observation and biochemical analyses suggested that Fat1p is present in the peroxisome and Faa1p is localized in the cytosol and to membranes.

  18. Digestive and metabolic utilization of lauric, myristic and stearic acid in cows, and associated effects on milk fat quality.

    Science.gov (United States)

    Dohme, Frigga; Machmüller, Andrea; Sutter, F; Kreuzer, M

    2004-04-01

    In an experiment with 3 x 6 Brown Swiss cows, the effects of dietary supplementations (40 g/kg) of non-esterified lauric (12 : 0), myristic (14 : 0) and stearic acid (18 : 0) on digestibility, metabolisability, milk fat composition and melting properties were investigated. The diet consisted of forage and concentrate in a ratio of 3 : 2. Cows were fed the C18 : 0 supplemented diet for 10 days before treatment feeding started for a 15-day experimental period where, at the end, excreta were quantitatively collected and gaseous exchange was measured. The DM intake averaged 17.9 kg/d for the C14 : 0 and C18 : 0 diets and was reduced (P acid supplementation were clearly reflected in the milk fat composition. Associated changes were elevated CLA and C18 : 1 trans when supplementing C12 : 0, and a high C18 : 1 to C16 : 0 ratio (P < 0.05) in the C12 : 0and C18 : 0 groups which resulted in an easier melting milk fat than with supplementary C14 : 0. Despite certain favourable effects of C12 : 0 in metabolic energy utilization and milk fat melting properties (relative to C14 : 0), more research is needed on how to improve its palatability for dairy cows.

  19. Effect of plate preparation on active-material utilization and cycleability of positive plates in automotive lead/acid batteries

    Science.gov (United States)

    Ozgun, H.; Lam, L. T.; Rand, D. A. J.; Bhargava, S. K.

    The power demands from automotive lead/acid batteries are rising steadily with the increasing number of electronic accessories that are being fitted to modern vehicles. In order to meet new levels of performance, automotive batteries have been redesigned to use low-ohmic microporous separators, as well as thinner plates (to increase the number of plates per cell) that are made with a low paste density. This approach, however, has led to a separate problem, namely, an appreciable reduction in battery service life. To redress this situation, a research programme has been implemented in our laboratories to examine, in detail, the effect of plate preparation on the active-material utilization and cycleability of automotive positive plates with grids made from low-antimony alloy. The cycleability is evaluated in terms of repetitive reserve-capacity. The results suggest that a paste formula with a combination of high density and low acid-to-oxide ratio is the most appropriate technology for the production of the thin positive plates that are required in advanced designs of automotive batteries.

  20. Fractionation of fish oil fatty acid methyl esters by means of argentation and reversed-phase high-performance liquid chromatography, and its utility in total fatty acid analysis

    NARCIS (Netherlands)

    Özcimder, M.; Hammers, W.E.

    1980-01-01

    The utility of reversed-phase and argentation high-performance liquid chromatography (HPLC) as pre-fractionation methods in fatty acid analysis is discussed. Both HPLC modes were applied to cod liver oil fatty acid methyl esters. Apart from positional isomers, the fractions obtained by reversed-phas

  1. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Kingsley, Mark T.

    2001-03-13

    The threat to American interests from terrorists is not limited to attacks against humans. Terrorists might seek to inflict damage to the U.S. economy by attacking our agricultural sector. Infection of commodity crops by bacterial or fungal crop pathogens could adversely impact U.S. agriculture, either directly from damage to crops or indirectly from damage to our ability to export crops suspected of contamination. Recognizing a terrorist attack against U.S. agriculture, to be able to prosecute the terrorists, is among the responsibilities of the members of Hazardous Material Response Unit (HMRU) of the Federal Bureau of Investigation (FBI). Nucleic acid analysis of plant pathogen strains by the use of polymerase chain reaction (PCR) amplification techniques is a powerful method for determining the exact identity of pathogens, as well as their possible region of origin. This type of analysis, however, requires that PCR assays be developed specific to each particular pathogen strain, and analysis protocols developed that are specific to the particular instrument used for detection. The objectives of the work described here were threefold: 1) to assess the potential terrorist threat to U.S. agricultural crops, 2) to determine whether suitable assays exist to monitor that threat, and 3) where assays are needed for priority plant pathogen threats, to modify or develop those assays for use by specialists at the HMRU. The assessment of potential threat to U.S. commodity crops and the availability of assays for those threats were described in detail in the Technical Requirements Document (9) and will be summarized in this report. This report addresses development of specific assays identified in the Technical Requirements Document, and offers recommendations for future development to ensure that HMRU specialists will be prepared with the PCR assays they need to protect against the threat of economic terrorism.

  2. Utilization of Condensed Distillers Solubles as Nutrient Supplement for Production of Nisin and Lactic Acid from Whey

    Science.gov (United States)

    Liu, Chuanbin; Hu, Bo; Chen, Shulin; Glass, Richard W.

    The major challenge associated with the rapid growth of the ethanol industry is the usage of the coproducts, i.e., condensed distillers solubles (CDS) and distillers dried grains, which are currently sold as animal feed supplements. As the growth of the livestock industries remains flat, alternative usage of these coproducts is urgently needed. CDS is obtained after the removal of ethanol by distillation from the yeast fermentation of a grain or a grain mixture by condensing the thin stillage fraction to semisolid. In this work, CDS was first characterized and yeast biomass was proven to be the major component of CDS. CDS contained 7.50% crude protein but with only 42% of that protein being water soluble. Then, CDS was applied as a nutrient supplement for simultaneous production of nisin and lactic acid by Lactococcus lactis subsp. lactis (ATCC 11454). Although CDS was able to support bacteria growth and nisin production, a strong inhibition was observed when CDS was overdosed. This may be caused by the existence of the major ethanol fermentation byproducts, especially lactate and acetate, in CDS. In the final step, the CDS based medium composition for nisin and lactic acid production was optimized using response surface methodology.

  3. Mandelic acid chiral separation utilizing a two-phase partitioning bioreactor built by polysulfone microspheres and immobilized enzymes.

    Science.gov (United States)

    Wang, Xinyu; Cui, Yanjun; Chen, Xia; Zhu, Hao; Zhu, Weiwei; Li, Yanfeng

    2015-03-01

    A novel two-phase partitioning bioreactor (TPPB) modified by polysulfone (PSF) microspheres and immobilized enzyme (novozym-435) was formed, and the resulting TPPB was applied into mandelic acid chiral separation. The PSF microspheres containing n-hexanol (named PSF/hexanol microspheres) was prepared by using the phase inversion method, which was used as the organic phase. Meanwhile, the immobilized enzyme novozym-435 was used as a biocatalyst. The water phase was composed of the phosphate buffer solution (PBS). (R, S)-Methyl mandelate was selected as the substrate to study enzymatic properties. Different reaction factors have been researched, such as pH, reaction time, temperature and the quantity of biocatalyst and PSF/hexanol microspheres added in. Finally, (S)-mandelic acid was obtained with an 80 % optical purity after 24 h in the two-phase partitioning bioreactor. The enantiomeric excess (eep) values were very low in the water phase, in which the highest eep value was only 46 %. The eep of the two-phase partitioning bioreactor had been enhanced more obviously than that catalyzed in the water phase.

  4. Evaluation of Final Radiological Conditions at Areas of the Niagara Falls Storage Site Remediated under the Formerly Utilized Sites Remedial Action Program -12184

    Energy Technology Data Exchange (ETDEWEB)

    Clayton, Christopher [U.S Department of Energy Office of Legacy Management, Washington, DC; Kothari, Vijendra [U.S Department of Energy Office of Legacy Management, Morgantown, West Virginia; Starr, Ken [U.S Department of Energy Office of Legacy Management, Westminster, Colorado; Widdop, Michael; Gillespie, Joey [SM Stoller Corporation, Grand Junction, Colorado

    2012-02-26

    The U. S. Department of Energy (DOE) methods and protocols allow evaluation of remediation and final site conditions to determine if remediated sites remain protective. Two case studies are presented that involve the Niagara Falls Storage Site (NFSS) and associated vicinity properties (VPs), which are being remediated under the Formerly Utilized Sites Remedial Action Program (FUSRAP). These properties are a part of the former Lake Ontario Ordnance Works (LOOW). In response to stakeholders concerns about whether certain remediated NFSS VPs were putting them at risk, DOE met with stakeholders and agreed to evaluate protectiveness. Documentation in the DOE records collection adequately described assessed and final radiological conditions at the completed VPs. All FUSRAP wastes at the completed sites were cleaned up to meet DOE guidelines for unrestricted use. DOE compiled the results of the investigation in a report that was released for public comment. In conducting the review of site conditions, DOE found that stakeholders were also concerned about waste from the Separations Process Research Unit (SPRU) at the Knolls Atomic Power Laboratory (KAPL) that was handled at LOOW. DOE agreed to determine if SPRU waste remained at that needed to be remediated. DOE reviewed records of waste characterization, historical handling locations and methods, and assessment and remediation data. DOE concluded that the SPRU waste was remediated on the LOOW to levels that pose no unacceptable risk and allow unrestricted use and unlimited exposure. This work confirms the following points as tenets of an effective long-term surveillance and maintenance (LTS&M) program: Stakeholder interaction must be open and transparent, and DOE must respond promptly to stakeholder concerns. DOE, as the long-term custodian, must collect and preserve site records in order to demonstrate that remediated sites pose no unacceptable risk. DOE must continue to maintain constructive relationships with the U

  5. Monola oil versus canola oil as a fish oil replacer in rainbow trout feeds: effects on growth, fatty acid metabolism and final eating quality.

    Science.gov (United States)

    Turchini, G M; Moretti, V M; Hermon, K; Caprino, F; Busetto, M L; Bellagamba, F; Rankin, T; Keast, R S J; Francis, D S

    2013-11-15

    Monola oil, a high oleic acid canola cultivar, and canola oil were evaluated as replacers of fish oil at three levels of inclusion (60%, 75% and 90%) in rainbow trout diets. After a 27-week grow-out cycle, the diet-induced effects on growth, fatty acid metabolism and final eating quality were assessed. Overall, no effects were noted for growth, feed utilisation or fish biometry, and the fatty acid composition of fish fillets mirrored that of the diets. Dietary treatments affected fillet lipid oxidation (free malondialdehyde), pigmentation and flavour volatile compounds, but only minor effects on sensorial attributes were detected. Ultimately, both oils were demonstrated to possess, to differing extents, suitable qualities to adequately replace fish oil from the perspective of fish performance and final product quality. However, further research is required to alleviate on-going issues associated with the loss of health promoting attributes (n-3 long chain polyunsaturated fatty acids) of final farmed products.

  6. Apical microleakage of different root canal sealers after use of maleic acid and EDTA as final irrigants.

    Science.gov (United States)

    Ulusoy, Ozgür Ilke; Nayir, Yelda; Celik, Kezban; Yaman, Sis Darendeliler

    2014-01-01

    This study aimed to compare the effects of ethylenediaminetetraacetic acid (EDTA) and maleic acid (MA) on the sealing ability of various root canal sealers. Eighty root canals were instrumented and irrigated with either EDTA or MA. They were divided into eight experimental groups and obturated as follows: Group 1: MA + Hybrid Root SEAL/gutta-percha. Group 2: EDTA + Hybrid Root SEAL/gutta-percha. Group 3: MA + iRoot SP/gutta-percha. Group 4: EDTA + iRoot SP/gutta-percha. Group 5: MA + EndoREZ/EndoREZ points. Group 6: EDTA + EndoREZ/EndoREZ points. Group 7: MA + AH Plus/gutta-percha. Group 8: EDTA + AH Plus/gutta-percha. Another ten roots were used as negative and positive controls. The microleakage of each sample was measured at 2-min intervals for 8 min using the fluid filtration method. Data were statistically analyzed with one-way ANOVA, post-hoc Tukey, and paired-samples t tests. The minimum microleakage values were obtained from the teeth obturated with AH Plus and EndoREZ selaers (p EDTA in terms of microleakage (p EDTA. The type of final irrigation solution seems to influence the postobturation apical seal. Use of AH Plus and EndoREZ sealers showed better sealing ability compared with IRoot SP and Hybrid Root SEAL.

  7. Simultaneous utilization of glucose and mannose from woody hydrolysate for free fatty acid production by metabolically engineered Escherichia coli.

    Science.gov (United States)

    Wu, Hui; Lee, Jane; Karanjikar, Mukund; San, Ka-Yiu

    2015-06-01

    In this study, the Escherichia coli strain MG1655 with fadD mutant (named as ML103), and MG1655 with fadD and ptsG double mutant (named as ML190) carrying the plasmid with the acyl-ACP thioesterase (TE) from Ricinus communis (pXZ18) or the plasmid with the combination of the TE and the native (3R)-hydroxyacyl-ACP dehydrase (fabZ) (pXZ18Z), produced free fatty acids (FFAs) efficiently using mannose as the sole carbon source. Due to the carbon catabolite repression (CCR) regulation, ML103(pXZ18) utilized glucose and mannose sequentially in the mixed sugar culture, while ML190(pXZ18) and ML190(pXZ18Z), with ptsG mutation, used glucose and mannose simultaneously. The highest total FFA concentration from the mixed sugar culture reached 2.96g/L by ML190(pXZ18Z). Furthermore, the strain ML190(pXZ18Z) can produce 2.86g/L FFAs with a high yield of 0.23g/g using hydrolysate mainly contained glucose and mannose from a commercial plant.

  8. Highly efficient rice straw utilization for poly-(γ-glutamic acid) production by Bacillus subtilis NX-2.

    Science.gov (United States)

    Tang, Bao; Lei, Peng; Xu, Zongqi; Jiang, Yongxiang; Xu, Zheng; Liang, Jinfeng; Feng, Xiaohai; Xu, Hong

    2015-10-01

    Lignocellulosic biomass has been identified as an economic and environmental feedstock for future biotechnological production. Here, for the first time, poly-(γ-glutamic acid) (PGA) production by Bacillus subtilis NX-2 using rice straw is investigated. Based on two-stage hydrolysis and characteristic consumption of xylose and glucose by B. subtilis NX-2, a co-fermentation strategy was designed to better accumulate PGA in a 7.5L fermentor by two feeding methods. The maximum cumulative respective PGA production and PGA productivity were 73.0 ± 0.5 g L(-1) and 0.81 g L(-1) h(-1) by the continuous feeding method, with carbon source cost was saved by 84.2% and 42.5% compared with glucose and cane molasse, respectively. These results suggest that rice straw, a type of abundant, low-cost, non-food lignocellulosic feedstock, may be feasibly and efficiently utilized for industrial-scale production of PGA. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. A fermentative approach towards optimizing directed biosynthesis of fumaric acid by Rhizopus oryzae 1526 utilizing apple industry waste biomass.

    Science.gov (United States)

    Das, Ratul Kumar; Brar, Satinder Kaur; Verma, Mausam

    2015-12-01

    The present research account deals with the bioproduction of fumaric acid (FA) from apple pomace ultrafiltration sludge (APUS) and apple pomace (AP) through fermentation. The filamentous fungus Rhizopus oryzae 1526 was used as a biocatalyst and its morphological impact on FA production was analysed in detail. For submerged fermentation, 40 g L(-1) of total solids concentration of APUS, pH 6.0, 30 °C, 200 rpm flask shaking speed and 72 h of incubation were found to be optimum for FA production (25.2 ± 1.0 g L(-1), 0.350 g (L(-1) h(-1))). Broth viscosity (cP), residual reducing sugar (g L(-1)) and ethanol (g L(-1)) produced as by-product, were also analysed. Plastic trays were used for solid state fermentation and at optimized level of moisture and incubation period, 52 ± 2.67 g FA per kg dry weight of AP was obtained. Changes in the total phenolic content (mg g(-1) dry weight of AP) were monitored at regular intervals. Utilization of APUS and AP for the directed synthesis of the high-value platform chemical FA by the fungal strain R. oryzae 1526 was an excellent display of fungal physiological and morphological control over a fermentative product.

  10. Pseudomonas lini Strain ZBG1 Revealed Carboxylic Acid Utilization and Copper Resistance Features Required for Adaptation to Vineyard Soil Environment: A Draft Genome Analysis

    Science.gov (United States)

    Chan, Kok-Gan; Chong, Teik-Min; Adrian, Tan-Guan-Sheng; Kher, Heng Leong; Grandclément, Catherine; Faure, Denis; Yin, Wai-Fong; Dessaux, Yves; Hong, Kar-Wai

    2016-01-01

    Pseudomonas lini strain ZBG1 was isolated from the soil of vineyard in Zellenberg, France and the draft genome was reported in this study. Bioinformatics analyses of the genome revealed presence of genes encoding tartaric and malic acid utilization as well as copper resistance that correspond to the adaptation this strain in vineyard soil environment. PMID:27512520

  11. Diurnal variation in insulin-stimulated systemic glucose and amino acid utilization in pigs fed with identical meals at 12-hour intervals

    NARCIS (Netherlands)

    Koopmans, S.J.; Meulen, van der J.; Dekker, R.A.; Corbijn, H.; Mroz, Z.

    2006-01-01

    The diurnal variation in insulin-stimulated systemic glucose and amino acid utilization was investigated in eleven pigs of similar to 40 kg. Pigs were fed isoenergetic/isoproteinic diets (366kj/kg BW0.75 per meal) in two daily rations (06:00 and 18:00h). After a 3-week habituation period, hyperinsul

  12. Role of Burkholderia pseudomallei Sigma N2 in Amino Acids Utilization and in Regulation of Catalase E Expression at the Transcriptional Level

    Directory of Open Access Journals (Sweden)

    Duong Thi Hong Diep

    2015-01-01

    Full Text Available Burkholderia pseudomallei is the causative agent of melioidosis. The complete genome sequences of this pathogen have been revealed, which explain some pathogenic mechanisms. In various hostile conditions, for example, during nitrogen and amino acid starvation, bacteria can utilize alternative sigma factors such as RpoS and RpoN to modulate genes expression for their adaptation and survival. In this study, we demonstrate that mutagenesis of rpoN2, which lies on chromosome 2 of B. pseudomallei and encodes a homologue of the sigma factor RpoN, did not alter nitrogen and amino acid utilization of the bacterium. However, introduction of B. pseudomallei rpoN2 into E. coli strain deficient for rpoN restored the ability to utilize amino acids. Moreover, comparative partial proteomic analysis of the B. pseudomallei wild type and its rpoN2 isogenic mutant was performed to elucidate its amino acids utilization property which was comparable to its function found in the complementation assay. By contrast, the rpoN2 mutant exhibited decreased katE expression at the transcriptional and translational levels. Our finding indicates that B. pseudomallei RpoN2 is involved in a specific function in the regulation of catalase E expression.

  13. Apical microleakage of different root canal sealers after use of maleic acid and EDTA as final irrigants

    Directory of Open Access Journals (Sweden)

    Özgür İlke ULUSOY

    2014-01-01

    Full Text Available This study aimed to compare the effects of ethylenediaminetetraacetic acid (EDTA and maleic acid (MA on the sealing ability of various root canal sealers. Eighty root canals were instrumented and irrigated with either EDTA or MA. They were divided into eight experimental groups and obturated as follows: Group 1: MA + Hybrid Root SEAL/gutta-percha. Group 2: EDTA + Hybrid Root SEAL/gutta-percha. Group 3: MA + iRoot SP/gutta-percha. Group 4: EDTA + iRoot SP/gutta-percha. Group 5: MA + EndoREZ/EndoREZ points. Group 6: EDTA + EndoREZ/EndoREZ points. Group 7: MA + AH Plus/gutta-percha. Group 8: EDTA + AH Plus/gutta-percha. Another ten roots were used as negative and positive controls. The microleakage of each sample was measured at 2-min intervals for 8 min using the fluid filtration method. Data were statistically analyzed with one-way ANOVA, post-hoc Tukey, and paired-samples t tests. The minimum microleakage values were obtained from the teeth obturated with AH Plus and EndoREZ selaers (p < 0.001. The samples with Hybrid Root SEAL showed the maximum leakage (p < 0.001. There were significant differences between the groups irrigated with MA or EDTA in terms of microleakage (p < 0.05. Use of MA resulted in higher microleakage values compared with those using EDTA. The type of final irrigation solution seems to influence the postobturation apical seal. Use of AH Plus and EndoREZ sealers showed better sealing ability compared with IRoot SP and Hybrid Root SEAL.

  14. 76 FR 33243 - Sulfanilic Acid From India; Final Results of Expedited Sunset Review of Countervailing Duty Order

    Science.gov (United States)

    2011-06-08

    ... production of optical brighteners, food colors, specialty dyes, and concrete additives. The principal... sulfanilic acid (sodium sulfanilate). Sulfanilic acid is a synthetic organic chemical produced from...

  15. Dietary Amino Acid Deficiency Reduces the Utilization of Amino Acids for Growth in Growing Pigs after a Period of Poor Health.

    Science.gov (United States)

    Kampman-van de Hoek, Esther; Jansman, Alfons J M; van den Borne, Joost J G C; van der Peet-Schwering, Carola M C; van Beers-Schreurs, Hetty; Gerrits, Walter J J

    2016-01-01

    During immune system activation, partitioning of amino acids (AAs) changes between protein gain and use by the immune system. We determined the effects of health status and dietary AA deficiency on nitrogen retention and AA utilization in pigs. Barrows (55 d of age) were obtained from good health (GH, n = 14) or poor health (PH, n = 14) status farms and allocated to a diet either adequate in essential amino acids (Adq) or 25% deficient in Met + Cys, Thr, and Trp (Def). Nitrogen balance was measured and AA irreversible loss rates (ILRs) were measured after an intravenous bolus of U-(13)C-labeled L-AAs. On arrival at the experimental facilities, the PH pigs had 14% lower serum albumin and 50% greater serum haptoglobin and blood leukocyte counts than the GH pigs (P < 0.01), but the PH pigs showed signs of recovery during the trial. Total tract nitrogen digestibility was 3 percentage points lower in the PH pigs (P < 0.01). The PH-Adq pigs had compensatory body weight gain after arrival, coinciding with 7% greater nitrogen retention (P < 0.01) in the PH pigs than in the GH pigs. The PH pigs had a 24% greater ILR for Lys. Health status × diet interactions for Lys (P = 0.07), Val (P = 0.03), and Leu (P = 0.10) pool sizes and a greater urea pool size in the PH pigs (P = 0.01) support the observation that the increase in the ILR of Lys in the PH pigs was related to oxidation when feeding the Def diet, but to synthesis when feeding the Adq diet. Feeding Def diets increased monocyte counts by 30% (P < 0.01). This study illustrates how the competition for AAs between protein synthesis associated with immune system activation and body protein deposition is greater when the dietary supply of Met + Cys, Thr, and Trp is limited in pigs during and after a period of poor health. © 2016 American Society for Nutrition.

  16. Clinical utility of automated platelet clump count in the screening for ethylene diamine tetraacetic acid-dependent pseudothrombocytopenia

    Institute of Scientific and Technical Information of China (English)

    WU Wei; GUO Ye; ZHANG Lin; CUI Wei; LI Wei; ZHANG Shuo

    2011-01-01

    Background Platelet (PLF) clumping occurring in pseudothrombocytopenia (PTCP) can result in inaccurate PLT.Automated platelet clump count (APCC) is a quantitative parameter of platelet aggregation.In this study,we evaluated the clinical utility of APCC in the screening for platelet aggregation related ethylene diamine tetraacetic acid (EDTA)-dependent PTCP (EDTA-PTCP).Methods A total of 105 patients and 200 healthy individuals were enrolled in this study.Blood samples were collected with dipotassium EDTA and sodium citrate respectively.ADVIA 2120 hematology analyzer was used to perform complete blood count (CBC) and APCC.Blood smears of both EDTA- and citrate-anticoagulated samples were made for microscope observation and manual PLT counting.Results In 25 patients with EDTA-PTCP patients,for EDTA-2K anticoagulated-blood,PLT was (55±6)×109/L,significantly lower than citrate anticoagulated blood ((186±13)×109/L)).APCC was (905±694)×109/L,significantly higher than citrate anticoagulated blood (98±37)×109/L.In true thrombocytopenia and healthy control groups,APCC was (63±60)×109/L and (69±59)×109/L respectively and there was no significant difference between EDTA and citrate anticoagulants.Receiver operator characteristic (ROC) curve showed both sensitivity and specificity of APCC were 96% when the cutoff value of APCC was set as 182×109/L.Other platelet parameters had poor performance.Conclusion The APCC has a good sensitivity and specificity in differentiating EDTA-PTCP from true thrombocytopenia compared with other platelet parameters.

  17. 76 FR 45510 - Sulfanilic Acid From India and the People's Republic of China; Final Results of Third Expedited...

    Science.gov (United States)

    2011-07-29

    ... sulfanilic acid. Sulfanilic acid is a synthetic organic chemical produced from the direct sulfonation of aniline with sulfuric acid. Sulfanilic acid is used as a raw material in the production of optical brighteners, food colors, specialty dyes, and concrete additives. The principal differences between the...

  18. 77 FR 9891 - Citric Acid and Certain Citrate Salts from the People's Republic of China: Amended Final Results...

    Science.gov (United States)

    2012-02-21

    ... International Trade Administration Citric Acid and Certain Citrate Salts from the People's Republic of China... antidumping duty order on citric acid and certain citrate salts (``citric acid'') from the People's Republic... Act of 1930, as amended (``the Act''). \\1\\ See Citric Acid and Certain Citrate Salts from the...

  19. Analysis of the utilization history and the planning and the participation profiles of the shaft plant Asse II. Final report; Analyse der Nutzungsgeschichte und der Planungs- und Beteiligungsformen der Schachtanlage Asse II. Endbericht

    Energy Technology Data Exchange (ETDEWEB)

    Ipsen, Detlev; Kost, Susanne; Weichler, Holger

    2010-03-08

    The report on the utilization history of Asse II covers the following issues: Historical facts of the purchase of the shaft plant Asse II, decision sequences and line of arguments, research mine or final repository? Emplacement of radioactive materials (inventory), stability of the mine layout and water ingress, risk assessment - accident analysis, communication and public information, conclusions and recommendations.

  20. Effect of the ratio between essential and nonessential amino acids in the diet on utilization of nitrogen and amino acids by growing pigs

    NARCIS (Netherlands)

    Lenis, N.P.; Diepen, van H.T.M.; Bikker, P.; Jongbloed, A.W.; Meulen, van der J.

    1999-01-01

    In 36 growing pigs (30 to 60 kg), N balance and amino acid (AA) composition of weight gain were measured to evaluate the interactive effect of the ratio between N from essential amino acids (EAA(N)) to nonessential amino acids (NEAA(N)) and total N level (T(N)) in the diet on N retention and

  1. Evaluation of Final Radiological Conditions at Areas of the Niagara Falls Storage Site Remediated under the Formerly Utilized Sites Remedial Action Program - 12184

    Energy Technology Data Exchange (ETDEWEB)

    Clayton, Christopher [U.S Department of Energy Office of Legacy Management, Washington, DC (United States); Kothari, Vijendra [U.S Department of Energy Office of Legacy Management, Morgantown, West Virginia (United States); Starr, Ken [U.S Department of Energy Office of Legacy Management, Westminster, Colorado (United States); Widdop, Michael; Gillespie, Joey [SM Stoller Corporation, Grand Junction, Colorado (United States)

    2012-07-01

    The U.S. Department of Energy (DOE) methods and protocols allow evaluation of remediation and final site conditions to determine if remediated sites remain protective. Two case studies are presented that involve the Niagara Falls Storage Site (NFSS) and associated vicinity properties (VPs), which are being remediated under the Formerly Utilized Sites Remedial Action Program (FUSRAP). These properties are a part of the former Lake Ontario Ordnance Works (LOOW). In response to stakeholders concerns about whether certain remediated NFSS VPs were putting them at risk, DOE met with stakeholders and agreed to evaluate protectiveness. Documentation in the DOE records collection adequately described assessed and final radiological conditions at the completed VPs. All FUSRAP wastes at the completed sites were cleaned up to meet DOE guidelines for unrestricted use. DOE compiled the results of the investigation in a report that was released for public comment. In conducting the review of site conditions, DOE found that stakeholders were also concerned about waste from the Separations Process Research Unit (SPRU) at the Knolls Atomic Power Laboratory (KAPL) that was handled at LOOW. DOE agreed to determine if SPRU waste remained at that needed to be remediated. DOE reviewed records of waste characterization, historical handling locations and methods, and assessment and remediation data. DOE concluded that the SPRU waste was remediated on the LOOW to levels that pose no unacceptable risk and allow unrestricted use and unlimited exposure. This work confirms the following points as tenets of an effective long-term surveillance and maintenance (LTS and M) program: - Stakeholder interaction must be open and transparent, and DOE must respond promptly to stakeholder concerns. - DOE, as the long-term custodian, must collect and preserve site records in order to demonstrate that remediated sites pose no unacceptable risk. - DOE must continue to maintain constructive relationships with

  2. Hierarchical amino acid utilization and its influence on fermentation dynamics: rifamycin B fermentation using Amycolatopsis mediterranei S699, a case study

    DEFF Research Database (Denmark)

    Bapat, Prashant Madhusudhan; Das, D.; Sohoni, Sujata Vijay

    2006-01-01

    in predicting the sequence and kinetics of amino acid uptake in a case study involving Amycolatopsis mediterranei, an industrially important organism. This work also throws some light on amino acid transporters and their regulation in A. mediterranei. Further, cybernetic model based experimental strategy...... catabolite repression. The model hypothesizes that the organism is an optimal strategist and invests resources for the uptake of a substrate that are proportional to the returns. Results: Stoichiometric coefficients and kinetic parameters of the model were estimated experimentally for Amycolatopsis...... predicted simultaneous uptake of amino acids at low cas concentrations and sequential uptake at high cas concentrations. The simulated profile of the key enzymes implies the presence of specific transporters for small groups of amino acids. Conclusion: The work demonstrates utility of the cybernetic model...

  3. Utilization of acetic acid-rich pyrolytic bio-oil by microalga Chlamydomonas reinhardtii: reducing bio-oil toxicity and enhancing algal toxicity tolerance.

    Science.gov (United States)

    Liang, Yi; Zhao, Xuefei; Chi, Zhanyou; Rover, Marjorie; Johnston, Patrick; Brown, Robert; Jarboe, Laura; Wen, Zhiyou

    2013-04-01

    This work was to utilize acetic acid contained in bio-oil for growth and lipid production of the microalga Chlamydomonas reinhardtii. The acetic acid-rich bio-oil fraction derived from fast pyrolysis of softwood contained 26% (w/w) acetic acid, formic acid, methanol, furfural, acetol, and phenolics as identified compounds, and 13% (w/w) unidentified compounds. Among those identified compounds, phenolics were most inhibitory to algal growth, followed by furfural and acetol. To enhance the fermentability of the bio-oil fraction, activated carbon was used to reduce the toxicity of the bio-oil, while metabolic evolution was used to enhance the toxicity tolerance of the microalgae. Combining activated carbon treatment and using evolved algal strain resulted in significant algal growth improvement. The results collectively showed that fast pyrolysis-fermentation process was a viable approach for converting biomass into fuels and chemicals. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. UTILIZATION OF MICRO SISAL FIBERS AS REINFORCEMENT AGENT AND POLYPROPYLENE OR POLYLACTIC ACID AS POLYMER MATRICES IN BIOCOMPOSITES MANUFACTURE

    Directory of Open Access Journals (Sweden)

    Subyakto Subyakto

    2013-06-01

    Full Text Available Sisal (Agave sisalana as a perennial tropical plant grows abundantly in Indonesia. Its fibers can be used as the reinforcement agent of biocomposite products. Utilization of sisal as natural fiber has some notable benefits compared to synthetic fibers, such as renewable, light in weight, and low in cost. Manufacture of biocomposite requires the use of matrix such as thermoplastic polymer, e.g. polypropylene (PP and polylactic acid (PLA to bond together with the reinforcement agent (e.g. sisal fibers. In relevant, experiment was conducted on biocomposites manufacture that comprised sisal fibers and PP as well as PLA. Sisal fibers were converted into pulp, then refined to micro-size fibrillated fibers such that their diameter reduced to about 10 μm, and dried in an oven. The dry microfibrillated sisal pulp fibers cellulose (MSFC were thoroughly mixed with either PP or PLA with varying ratios of MSFC/PP as well as MSFC/PLA, and then shaped into the mat (i.e. MSFC-PP and MSFC-PLA biocomposites. Two kinds of shaping was employed, i.e. hot-press molding and injection molding. In the hot-press molding, the ratio of  MSFC/PP as well as MSFC/PLA ranged about 30/70-50/50. Meanwhile in the injection (employed only on assembling the MSFC-PLA biocomposite, the ratio of MSFC/PLA varied about 10/90-30/70. The resulting shaped MSFC-PP and MSFC-PLA biocomposites were then tested of its physical and mechanical properties. With the hot-press molding device, the physical and mechanical (strength properties of MSFC-PLA biocomposite were higher than those of  MSFC-PP biocomposite. The optimum ratio of  MSFC/PP as well as MSFC/PLA reached concurrently at 40/60. The strengths of MSFC-PP as well as MSFC-PLA biocomposites were greater than those of individual polymer (PP and PLA. With the injection molding device, only the MSFC-PLA  biocomposite  was formed  and its strengths  reached  maximum  at 30/70  ratio.  The particular strengths (MOR and MOE of MSFC

  5. Continuous-Flow Electrophilic Amination of Arenes and Schmidt Reaction of Carboxylic Acids Utilizing the Superacidic Trimethylsilyl Azide/Triflic Acid Reagent System.

    Science.gov (United States)

    Chen, Yuesu; Gutmann, Bernhard; Kappe, C Oliver

    2016-10-07

    A continuous flow protocol for the direct stoichiometric electrophilic amination of aromatic hydrocarbons and the Schmidt reaction of aromatic carboxylic acids using the superacidic trimethylsilyl azide/triflic acid system is described. Optimization of reagent stoichiometry, solvent, reaction time, and temperature led to an intensified protocol at elevated temperatures that allows the direct amination of arenes to be completed within 3 min at 90 °C. In order to improve the selectivity and scope of this direct amination protocol, aromatic carboxylic acids were additionally chosen as substrates. Selected carboxylic acids could be converted to their corresponding amine counterparts in good to excellent yields (11 examples, 55-83%) via a Schmidt reaction employing similar flow reaction conditions (acid intermediate, the corrosive nature of triflic acid, and the exothermic quenching were addressed by designing a suitable continuous flow reaction setup for both types of transformations.

  6. Final Technical Report for Contract No. DE-EE0006332, "Integrated Simulation Development and Decision Support Tool-Set for Utility Market and Distributed Solar Power Generation"

    Energy Technology Data Exchange (ETDEWEB)

    Cormier, Dallas [San Diego Gas & Electric, CA (United States); Edra, Sherwin [San Diego Gas & Electric, CA (United States); Espinoza, Michael [San Diego Gas & Electric, CA (United States); Daye, Tony [Green Power Labs, San Diego, CA (United States); Kostylev, Vladimir [Green Power Labs, San Diego, CA (United States); Pavlovski, Alexandre [Green Power Labs, San Diego, CA (United States); Jelen, Deborah [Electricore, Inc., Valencia, CA (United States)

    2014-12-29

    This project will enable utilities to develop long-term strategic plans that integrate high levels of renewable energy generation, and to better plan power system operations under high renewable penetration. The program developed forecast data streams for decision support and effective integration of centralized and distributed solar power generation in utility operations. This toolset focused on real time simulation of distributed power generation within utility grids with the emphasis on potential applications in day ahead (market) and real time (reliability) utility operations. The project team developed and demonstrated methodologies for quantifying the impact of distributed solar generation on core utility operations, identified protocols for internal data communication requirements, and worked with utility personnel to adapt the new distributed generation (DG) forecasts seamlessly within existing Load and Generation procedures through a sophisticated DMS. This project supported the objectives of the SunShot Initiative and SUNRISE by enabling core utility operations to enhance their simulation capability to analyze and prepare for the impacts of high penetrations of solar on the power grid. The impact of high penetration solar PV on utility operations is not only limited to control centers, but across many core operations. Benefits of an enhanced DMS using state-of-the-art solar forecast data were demonstrated within this project and have had an immediate direct operational cost savings for Energy Marketing for Day Ahead generation commitments, Real Time Operations, Load Forecasting (at an aggregate system level for Day Ahead), Demand Response, Long term Planning (asset management), Distribution Operations, and core ancillary services as required for balancing and reliability. This provided power system operators with the necessary tools and processes to operate the grid in a reliable manner under high renewable penetration.

  7. Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum.

    Science.gov (United States)

    Svetlitshnyi, V; Rainey, F; Wiegel, J

    1996-10-01

    Three strains of an anaerobic thermophilic organoheterotrophic lipolytic alkalitolerant bacterium, Thermosyntropha lipolytica gen. nov., sp. nov. (type strain JW/VS-265T; DSM 11003), were isolated from alkaline hot springs of Lake Bogoria (Kenya). The cells were nonmotile, non-spore forming, straight or slightly curved rods. At 60 degrees C the pH range for growth determined at 25 degrees C [pH25 degrees C] was 7.15 to 9.5, with an optimum between 8.1 and 8.9 (pH60 degrees C of 7.6 and 8.1). At a pH25 degrees C of 8.5 the temperature range for growth was from 52 to 70 degrees C, with an optimum between 60 and 66 degrees C. The shortest doubling time was around 1 h. In pure culture the bacterium grew in a mineral base medium supplemented with yeast extract, tryptone, Casamino Acids, betaine, and crotonate as carbon sources, producing acetate as a major product and constitutively a lipase. During growth in the presence of olive oil, free long-chain fatty acids were accumulated in the medium but the pure culture could not utilize olive oil, triacylglycerols, short- and long-chain fatty acids, and glycerol for growth. In syntrophic coculture (Methanobacterium strain JW/VS-M29) the lipolytic bacteria grew on triacylglycerols and linear saturated and unsaturated fatty acids with 4 to 18 carbon atoms, but glycerol was not utilized. Fatty acids with even numbers of carbon atoms were degraded to acetate and methane, while from odd-numbered fatty acids 1 mol of propionate per mol of fatty acid was additionally formed. 16S rDNA sequence analysis identified Syntrophospora and Syntrophomonas spp. as closest phylogenetic neighbors.

  8. FadD Is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids ▿ †

    Science.gov (United States)

    Pech-Canul, Ángel; Nogales, Joaquina; Miranda-Molina, Alfonso; Álvarez, Laura; Geiger, Otto; Soto, María José; López-Lara, Isabel M.

    2011-01-01

    FadD is an acyl coenzyme A (CoA) synthetase responsible for the activation of exogenous long-chain fatty acids (LCFA) into acyl-CoAs. Mutation of fadD in the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti promotes swarming motility and leads to defects in nodulation of alfalfa plants. In this study, we found that S. meliloti fadD mutants accumulated a mixture of free fatty acids during the stationary phase of growth. The composition of the free fatty acid pool and the results obtained after specific labeling of esterified fatty acids with a Δ5-desaturase (Δ5-Des) were in agreement with membrane phospholipids being the origin of the released fatty acids. Escherichia coli fadD mutants also accumulated free fatty acids released from membrane lipids in the stationary phase. This phenomenon did not occur in a mutant of E. coli with a deficient FadL fatty acid transporter, suggesting that the accumulation of fatty acids in fadD mutants occurs inside the cell. Our results indicate that, besides the activation of exogenous LCFA, in bacteria FadD plays a major role in the activation of endogenous fatty acids released from membrane lipids. Furthermore, expression analysis performed with S. meliloti revealed that a functional FadD is required for the upregulation of genes involved in fatty acid degradation and suggested that in the wild-type strain, the fatty acids released from membrane lipids are degraded by β-oxidation in the stationary phase of growth. PMID:21926226

  9. Selective utilization of exogenous amino acids by Dehalococcoides ethenogenes strain 195 and its effects on growth and dechlorination activity.

    Science.gov (United States)

    Zhuang, Wei-Qin; Yi, Shan; Feng, Xueyang; Zinder, Stephen H; Tang, Yinjie J; Alvarez-Cohen, Lisa

    2011-11-01

    Bacteria of the genus Dehalococcoides are important members of bioremediation communities because of their ability to detoxify chloroethenes to the benign end product ethene. Genome-enabled studies conducted with Dehalococcoides ethenogenes 195 have revealed that two ATP-binding cassette (ABC)-type amino acid transporters are expressed during its exponential growth stages. In light of previous findings that Casamino Acids enhanced its dechlorination activity, we hypothesized that strain 195 is capable of importing amino acids from its environment to facilitate dechlorination and growth. To test this hypothesis, we applied isotopomer-based dilution analysis with (13)C-labeled acetate to differentiate the amino acids that were taken up by strain 195 from those synthesized de novo and to determine the physiological changes caused by the significantly incorporated amino acids. Our results showed that glutamate/glutamine and aspartate/asparagine were almost exclusively synthesized by strain 195, even when provided in excess in the medium. In contrast, phenylalanine, isoleucine, leucine, and methionine were identified as the four most highly incorporated amino acids, at levels >30% of respective proteinogenic amino acids. When either phenylalanine or all four highly incorporated amino acids were added to the defined mineral medium, the growth rates, dechlorination activities, and yields of strain 195 were enhanced to levels similar to those observed with supplementation with 20 amino acids. However, genes for the putative ABC-type amino acids transporters and phenylalanine biosynthesis exhibited insignificant regulation in response to the imported amino acids. This study also demonstrates that using isotopomer-based metabolite analysis can be an efficient strategy for optimizing nutritional conditions for slow-growing microorganisms.

  10. Evaluation of smear layer removal and marginal adaptation of root canal sealer after final irrigation using ethylenediaminetetraacetic, peracetic, and etidronic acids with different concentrations.

    Science.gov (United States)

    Ulusoy, Özgür İlke; Zeyrek, Salev; Çelik, Bülent

    2017-02-12

    The purpose of this study was to investigate the effects of different irrigation solutions on the smear layer removal and marginal adaptation of a resin-based sealer to root canal dentine. A total of 152 instrumented roots were irrigated with the following irrigants: 9,18% etidronic acid (HEBP), 0.5, 1,2% peracetic acid (PAA), 17% ethylenediaminetetraacetic acid (EDTA), saline. The amount of smear layer was evaluated using scanning electron microscope (SEM) in seventy root samples. Eighty-two roots were filled with AH Plus and gutta-percha. Slices obtained from apical third of each specimen were viewed with SEM to assess marginal adaptation. Use of 9% and 18% HEBP resulted in more efficient smear layer removal in the apical third than the other chelators (p acid is a promising candidate for final irrigation of root canals.

  11. L-Lactic acid production by combined utilization of agricultural bioresources as renewable and economical substrates through batch and repeated-batch fermentation of Enterococcus faecalis RKY1.

    Science.gov (United States)

    Reddy, Lebaka Veeranjaneya; Kim, Young-Min; Yun, Jong-Sun; Ryu, Hwa-Won; Wee, Young-Jung

    2016-06-01

    Enterococcus faecalis RKY1 was used to produce l-lactic acid from hydrol, soybean curd residues (SCR), and malt. Hydrol was efficiently metabolized to l-lactic acid with optical purity of >97.5%, though hydrol contained mixed sugars such as glucose, maltose, maltotriose, and maltodextrin. Combined utilization of hydrol, SCR, and malt was enough to sustain lactic acid fermentation by E. faecalis RKY1. In order to reduce the amount of nitrogen sources and product inhibition, cell-recycle repeated-batch fermentation was employed, where a high cell mass (26.3g/L) was obtained. Lactic acid productivity was improved by removal of lactic acid from fermentation broth by membrane filtration and by linearly increased cell density. When the total of 10 repeated-batch fermentations were carried out using 100g/L hydrol, 150g/L SCR hydrolyzate, and 20g/L malt hydrolyzate as the main nutrients, lactic acid productivity was increased significantly from 3.20g/L/h to 6.37g/L/h.

  12. The efficiency of utilization of metabolizable energy and apparent absorption of amino acids in sheep given spring- and autumn-harvested dried grass.

    Science.gov (United States)

    Macrae, J C; Smith, J S; Dewey, P J; Brewer, A C; Brown, D S; Walker, A

    1985-07-01

    Three experiments were conducted with sheep given spring-harvested dried grass (SHG) and autumn-harvested dried grass (AHG). The first was a calorimetric trial to determine the metabolizable energy (ME) content of each grass and the efficiency with which sheep utilize their extra ME intakes above the maintenance level of intake. The second examined the relative amounts of extra non-ammonia-nitrogen (NAN) and individual amino acids absorbed from the small intestine per unit extra ME intake as the level of feeding was raised from energy equilibrium (M) to approximately 1.5 M. The third was a further calorimetric trial to investigate the effect of an abomasal infusion of 30 g casein/d on the efficiency of utilization of AHG. The ME content of the SHG (11.8 MJ/kg dry matter (DM] was higher than that of AHG (10.0 MJ/kg DM). The efficiency of utilization of ME for productive purposes (i.e. above the M level of intake; kf) was higher when given SHG (kf 0.54 between M and 2 M) than when given AHG (kf 0.43 between M and 2 M). As the level of intake of each grass was raised from M to 1.5 M there was a greater increment in the amounts of NAN (P less than 0.001) and the total amino acid (P less than 0.05) absorbed from the small intestines when sheep were given the SHG (NAN absorption, SHG 5.4 g/d, AHG 1.5 g/d, SED 0.54; total amino acid absorption SHG 31.5 g/d, AHG 14.3 g/d, SED 5.24). Infusion of 30 g casein/d per abomasum of sheep given AHG at M and 1.5 M levels of intake increased (P less than 0.05) the efficiency of utilization of the herbage from kf 0.45 to kf 0.57. Consideration is given to the possibility that the higher efficiency of utilization of ME in sheep given SHG may be related to the amounts of extra glucogenic amino acids absorbed from the small intestine which provide extra reducing equivalents (NADPH) and glycerol phosphate necessary for the conversion of acetate into fatty acids.

  13. Application of utility theory to the valuing of air pollution-related health effects: Three proposed pilot studies on subjective judgments of asthma. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Johnston, A.C.

    1991-04-01

    Utility under uncertainty is a field of decision theory that has received increasing attention in the field of health. The report reviews its uses during the past decade and suggests its possible use in national air quality standard setting procedures. It is common practice in standard setting to assess the likelihood of air pollution effects on sensitive populations. One such poplation, asthmatics, is selected in the report and the relationship between air pollution and asthma is reviewed. In addition, three possible pilot studies are suggested which use aspects of utility under uncertainty theory to elicit values concerning asthma health effects. The results of such studies would provide the US EPA with information for their ambient air quality standard setting and increase the awareness of the possible uses of utility theory in such applications.

  14. Influence of an Escherichia coli-derived phytase on nutrient utilization in broiler starters fed diets containing varying concentrations of phytic acid.

    Science.gov (United States)

    Ravindran, V; Morel, P C H; Partridge, G G; Hruby, M; Sands, J S

    2006-01-01

    The influence of an Escherichia coli-derived phytase, on nutrient utilization was investigated in broilers fed starter diets containing different concentrations of phytate. The study was conducted as a 3 x 4 factorial arrangement of treatments with 3 concentrations of phytic acid (10.4, 11.8, and 13.6 g/kg; equivalent to 2.8, 3.3, and 3.8 g of phytate P/kg) and phytase (0, 500, 750, and 1,000 FTU/kg). One unit of phytase (FTU) is defined as the quantity of enzyme that releases 1 micromol of inorganic phosphorus/min from 0.00015 mol/L of sodium phytate at pH 5.5 at 37 degrees C. The dietary phytic acid concentrations were manipulated by the inclusion of rice bran. Increasing dietary concentrations of phytic acid resulted in reductions (P phytate concentrations. Apparent ileal digestibility coefficients of protein and most amino acids were influenced by phytate (P phytate concentrations, but the trend in responses to increasing phytase additions was different at different phytate concentrations as shown by significant phytate x phytase interactions (P phytate concentration, the ileal digestibility coefficients increased with increasing phytase supplementation. At the medium and high phytate concentrations, the greatest responses were observed at 500 FTU/kg of phytase, with little improvement attributable to further additions. Ileal digestibility of P was lowered (P phytate concentrations and increased (P phytate x phytase interaction (P phytate concentrations. These data demonstrate the anti-nutritive effects of phytic acid and the potential of microbial phytase to improve energy utilization and the availability of P and amino acids in broilers fed starter diets.

  15. A novel automated device for rapid nucleic acid extraction utilizing a zigzag motion of magnetic silica beads

    Energy Technology Data Exchange (ETDEWEB)

    Yamaguchi, Akemi [Graduate School of Science and Technology, Shinshu University, Nagano (Japan); Core Technology Development Center, Seiko Epson Corporation, Suwa (Japan); Matsuda, Kazuyuki, E-mail: kmatsuda@shinshu-u.ac.jp [Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto (Japan); Uehara, Masayuki [Core Technology Development Center, Seiko Epson Corporation, Suwa (Japan); Honda, Takayuki [Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto (Japan); Saito, Yasunori [Institute of Engineering, Academic Assembly, Shinshu University, Nagano (Japan)

    2016-02-04

    We report a novel automated device for nucleic acid extraction, which consists of a mechanical control system and a disposable cassette. The cassette is composed of a bottle, a capillary tube, and a chamber. After sample injection in the bottle, the sample is lysed, and nucleic acids are adsorbed on the surface of magnetic silica beads. These magnetic beads are transported and are vibrated through the washing reagents in the capillary tube under the control of the mechanical control system, and thus, the nucleic acid is purified without centrifugation. The purified nucleic acid is automatically extracted in 3 min for the polymerase chain reaction (PCR). The nucleic acid extraction is dependent on the transport speed and the vibration frequency of the magnetic beads, and optimizing these two parameters provided better PCR efficiency than the conventional manual procedure. There was no difference between the detection limits of our novel device and that of the conventional manual procedure. We have already developed the droplet-PCR machine, which can amplify and detect specific nucleic acids rapidly and automatically. Connecting the droplet-PCR machine to our novel automated extraction device enables PCR analysis within 15 min, and this system can be made available as a point-of-care testing in clinics as well as general hospitals. - Highlights: • Automatic nucleic acid extraction is performed in 3 min. • Zigzag motion of magnetic silica beads yields rapid and efficient extraction. • The present our device provides better performance than the conventional procedure.

  16. Factors affecting the potential of direct load control for non-generating utilities. Final report. [Distribution and wholesale power supply interaction

    Energy Technology Data Exchange (ETDEWEB)

    None

    1979-04-01

    Several alternatives are available for achieving load management, including direct or voluntary control of customer loads, customer or utility energy storage systems for diurnal load shifting, and expanded interconnection and operation of electric power systems. All of these alternatives are available to the fully integrated (generating, transmitting and distributing) electric utility and the analysis of their effects encompasses the power supply and delivery system. However, the costs and benefits of the alternatives to the fully integrated electric utility are perhaps not so obvious. Therefore, by considering a non-generating utility, this analysis focuses upon the distribution system and wholesale power supply interaction as a step toward an analysis including the power supply and delivery system. This report develops an analysis procedure and discusses some of the relevant factors to be consdered in the application of direct load control for a non-generating utility system. The analysis concentrates on the distribution system only to determine the effect of rates and payback as a result of direct load control. Thus, the study is responsive to the specific needs of the non-generating utility. This analysis of direct load control encompasses the determination of those loads amenable to control, the selection of a suitable one-way communications system to rend control and the estimation of expected benefits and costs. The complementary functions to the application of direct load control such as automatic meter reading via the addition of a bi-directional communications system and voltage control are not included in the analysis but are detailed for future consideration.

  17. 77 FR 74171 - Citric Acid and Certain Citrate Salts From the People's Republic of China: Final Results of...

    Science.gov (United States)

    2012-12-13

    ... sodium citrate, otherwise known as citric acid sodium salt, and the monohydrate and monopotassium forms of potassium citrate.\\5\\ Sodium citrate also includes both trisodium citrate and monosodium citrate... acid and sodium citrate are classifiable under 2918.14.0000 and 2918.15.1000 of the Harmonized...

  18. A study of toxic emissions from a coal-fired power plant utilizing an ESP while demonstrating the ICCT CT-121 FGD Project. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1994-06-16

    The US Department of Energy is performing comprehensive assessments of toxic emissions from eight selected coal-fired electric utility units. This program responds to the Clean Air Act Amendments of 1990, which require the US Environmental Protection Agency (EPA) to evaluate emissions of hazardous air pollutants (HAPs) from electric utility power plants for Potential health risks. The resulting data will be furnished to EPA utility power plants and health risk determinations. The assessment of emissions involves the collection and analysis of samples from the major input, process, and output streams of each of the eight power plants for selected hazardous Pollutants identified in Title III of the Clean Air Act. Additional goals are to determine the removal efficiencies of pollution control subsystems for these selected pollutants and the Concentrations associated with the particulate fraction of the flue gas stream as a function of particle size. Material balances are being performed for selected pollutants around the entire power plant and several subsystems to identify the fate of hazardous substances in each utility system. Radian Corporation was selected to perform a toxics assessment at a plant demonstrating an Innovative Clean Coal Technology (ICCT) Project. The site selected is Plant Yates Unit No. 1 of Georgia Power Company, which includes a Chiyoda Thoroughbred-121 demonstration project.

  19. The Ability of Color-Vision Defective and Color-Normal Early Elementary and Junior High Students to Utilize Color. Final Report.

    Science.gov (United States)

    Cook, Ann C. N.; Hannaford, Alonzo E.

    The investigation was undertaken to obtain data on the number of kindergarten, second, and seventh grade Ss classified as having color vision defects by the American Optical-Hardy, Rand, Rittler Test (AO-HRR) and two tests by S. Ishihara. Also studied was the ability of color vision defective and color normal Ss to utilize color as measured by the…

  20. A novel automated device for rapid nucleic acid extraction utilizing a zigzag motion of magnetic silica beads.

    Science.gov (United States)

    Yamaguchi, Akemi; Matsuda, Kazuyuki; Uehara, Masayuki; Honda, Takayuki; Saito, Yasunori

    2016-02-04

    We report a novel automated device for nucleic acid extraction, which consists of a mechanical control system and a disposable cassette. The cassette is composed of a bottle, a capillary tube, and a chamber. After sample injection in the bottle, the sample is lysed, and nucleic acids are adsorbed on the surface of magnetic silica beads. These magnetic beads are transported and are vibrated through the washing reagents in the capillary tube under the control of the mechanical control system, and thus, the nucleic acid is purified without centrifugation. The purified nucleic acid is automatically extracted in 3 min for the polymerase chain reaction (PCR). The nucleic acid extraction is dependent on the transport speed and the vibration frequency of the magnetic beads, and optimizing these two parameters provided better PCR efficiency than the conventional manual procedure. There was no difference between the detection limits of our novel device and that of the conventional manual procedure. We have already developed the droplet-PCR machine, which can amplify and detect specific nucleic acids rapidly and automatically. Connecting the droplet-PCR machine to our novel automated extraction device enables PCR analysis within 15 min, and this system can be made available as a point-of-care testing in clinics as well as general hospitals.

  1. Obeticholic acid for the treatment of primary biliary cholangitis in adult patients: clinical utility and patient selection

    Directory of Open Access Journals (Sweden)

    Bowlus CL

    2016-09-01

    Full Text Available Christopher L Bowlus Division of Gastroenterology and Hepatology, University of California Davis, Davis, CA, USA Abstract: Primary biliary cholangitis (PBC, previously known as primary biliary “cirrhosis”, is a rare autoimmune liver disease characterized by the hallmark autoantibodies to mitochondrial antigens and immune-mediated destruction of small bile duct epithelial cells leading to cholestasis and cirrhosis. Surprisingly, while immune modulators have not been effective in the treatment of PBC, supplementation with the hydrophilic bile acid (BA ursodeoxycholic acid (UDCA has been demonstrated to slow the disease progression. However, a significant minority of PBC patients do not have a complete response to UDCA and remain at risk of continued disease progression. Although the mechanisms of action are not well understood, UDCA provided proof of concept for BA therapy in PBC. Obeticholic acid (OCA, a novel derivative of the human BA chenodeoxycholic acid, is a potent agonist of the nuclear hormone receptor farnesoid X receptor, which regulates BA synthesis and transport. A series of clinical trials of OCA in PBC, primarily in combination with UDCA, have established that OCA leads to significant reductions in serum alkaline phosphatase that are predicted to lead to improved clinical outcomes, while dose-dependent pruritus has been the most common adverse effect. On the basis of these studies, OCA was given conditional approval by the US Food and Drug Administration with plans to establish the long-term clinical efficacy of OCA in patients with advanced PBC. Keywords: primary biliary cholangitis, nuclear receptors, farnesoid X receptor, bile acid, obeticholic acid, ursodeoxycholic acid

  2. Impact of improving dietary amino acid balance for lactating sows on efficiency of dietary amino acid utilization and transcript abundance of genes encoding lysine transporters in mammary tissue

    DEFF Research Database (Denmark)

    Huber, L; de Lange, C F M; Ernst, Cathy

    2016-01-01

    ) Lys concentration was 80% of the estimated requirement. In Exp. 2, 24 sows were assigned to the HCP or LCP diets. In Exp. 1, blood samples were postprandially collected 15 h on d 3, 7, 14, and 18 of lactation and utilization efficiency of dietary AA for milk production was calculated during early (d 3...

  3. The Multi-Disciplinary Graduate Program in Educational Research. Final Report, Part IV; The Utilization of Sociological Ideas in Organizational Planning: A Case Study.

    Science.gov (United States)

    Lazarsfeld, Paul F., Ed.

    This document, the fourth in the final report on the Multi-Disciplinary Graduate Program in Educational Research, is a qualitative case study designed to show the form of sociological contributions to and the role of sociologists in policy formulation at an American Educational Research Association (AERA) colloquium. Discussions at the conference…

  4. Improved Cycling Performance of a Si Nanoparticle Anode Utilizing Citric Acid as a Surface-Modifying Agent.

    Science.gov (United States)

    Nguyen, Cao Cuong; Seo, Daniel M; Chandrasiri, K W D K; Lucht, Brett L

    2017-09-19

    Citric acid and its analogues have been investigated as surface-modifying agents for Si nanoparticle anodes using electrochemical cycling, attenuated total reflectance infrared (ATR IR), and X-ray photoelectron spectroscopy (XPS). A Si nanoparticle anode prepared with citric acid (CA) has better capacity retention than one containing 1,2,3,4-butanetetracarboxylic acid (BA), but both electrodes outperform Si-PVDF. The Si-CA anode has an initial specific capacity of 3530 mA h/g and a first cycle efficiency of 82%. Surprisingly, the Si-CA electrode maintains a high specific capacity of ∼2200 mA h/g after 250 cycles, corresponding to 64% capacity retention, which is similar to the Si prepared with long-chain poly(acrylic acid) (PAA). On the contrary, the silicon electrode prepared with PVDF has a fast capacity fade and retains only 980 mA h/g after 50 cycles. The IR and XPS data show that the Si-CA electrode has an SEI composed primarily of lithium citrate during the first 50 cycles, resulting from the electrochemical reduction of citric acid. Only low concentrations of electrolyte reduction products are observed. The lithium citrate layer derived from CA stabilizes the silicon surface and suppresses electrolyte reduction, which likely contributes to the enhanced cycling performance of the Si nanoparticle anode.

  5. The use of formaldehyde protected palm kernel meal and its effects on animal performance, nitrogen utilization and unsaturated fatty acid composition in Priangan sheep

    Directory of Open Access Journals (Sweden)

    K.G Wiryawan

    2007-12-01

    Full Text Available An experiment was conducted to investigate the effect of formaldehyde-treated Palm Kernel Cake (BIS in male Priangan sheep diet and its effect on animal performance, N utilization, and unsaturated fatty acids composition of musculus longisimus dorsal. The experiment was designed based on completly randomized design with four treatments and nine replications. The treatments consisted of four levels of formaldehyde treated BIS: R1 (0%, R2 (15%, R3 (30% and R4 (45%. Results indicated that formaldehyde treated BIS up to 45% in diet did not negatively affect consumption, dry matter digestibility and daily live weight gain. Formaldehyde protection especially in R4 could significantly reduce ruminal N-NH3 concentration, increased protein digestibility and nitrogen retention, but did not influence allantoin urine concentration. Unsaturated fatty acid composition of musculus longisimus dorsal was not affected by formaldehyde treated BIS in diet. It is concluded that the inclusion of 45% formaldehyde treated BIS in diet did not negatively affect animal performance, and R4 is the most effective in improving nitrogen utilization in male Priangan sheep, but unable to alter the unsaturated fatty acids composition of musculus longisimus dorsal.

  6. Production development and utilization of Zimmer Station wet FGD by-products. Final report. Volume 3, Product development of gypsum, Phase 1

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Kevin [Dravo Technology Center, Pittsburgh, PA (United States); Beeghly, Joel H. [Dravo Technology Center, Pittsburgh, PA (United States)

    2000-11-30

    In the way of background information about 30 electric utility units with a combined total of 15,000 MW utilize magnesium enhanced lime flue gas desulfurization (FGD) systems. The first generation process begun in 1973, called the Thiosorbic® Process, was a technical breakthrough that offered significantly improved operating and performance characteristics compared with competing FGD technologies. The process is described as Flow Diagram "A" in Figure 1. A disadvantage of this and other inhibited or natural oxidation wet FGD systems is the capital and operating cost associated with landfill disposal of the calcium sulfite based solids. Fixation to stabilize the sludge solids for compunction in a landfill also consumes fly ash that otherwise may be marketable.

  7. Production development and utilization of Zimmer Station wet FGD by-products. Final report. Volume 2, Product development of magnesium hydroxide, Phase 1

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Kevin [Dravo Technology Center, Pittsburgh, PA (United States); Beeghly, Joel H. [Dravo Technology Center, Pittsburgh, PA (United States)

    2000-11-30

    In the way of background information about 30 electric utility units with a combined total of 15,000 MW utilize magnesium enhanced lime flue gas desulfurization (FGD) systems. The first generation process begun in 1973, called the Thiosorbic® Process, was a technical breakthrough that offered significantly improved operating and performance characteristics compared with competing FGD technologies. The process is described as Flow Diagram "A" in figure 1. A disadvantage of this and other inhibited or natural oxidation wet FGD systems is the capital and operating cost associated with landfill disposal of the calcium sulfite based solids. Fixation to stabilize the sludge solids for compaction in a landfill also consumes fly ash that otherwise may be marketable.

  8. Examination of the factors and issues for an environmental technology utilization partnership between the private sector and the Department of Energy. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Brouse, P.

    1997-05-01

    The Department of Energy (DOE) held a meeting on November 12, 1992 to evaluate the DOE relations with industry and university partners concerning environmental technology utilization. The goal of this meeting was to receive feedback from DOE industry and university partners for the identification of opportunities to improve the DOE cooperative work processes with the private sector. The meeting was designed to collect information and to turn that information into action to improve private sector partnerships with DOE.

  9. Retrofit NO{sub x} controls for utility boilers: a synthesis of technologies, issues, and CAAA ozone attainment legislation: Final report 1993

    Energy Technology Data Exchange (ETDEWEB)

    Eskmazi, D.; Cichanowicz, E.; Kokkinos, A.; Stallings, J.; Offen, G. [Electric Power Research Institute, Palo Alto, CA (United States)

    1993-10-01

    This document brings together a set of summary papers on the status of NO{sub x} control technologies for complying with the requirements of the Clean Air Act Amendments (CAAA) of 1990. The individual papers were prepared in mid 1992 as synthesized information for utilities who were beginning to assess their compliance options and enter discussions with their regulatory agencies. Their purpose is to introduce utility engineers and managers, regulators, and the public to the available NO{sub x} reduction options, and to do so in a manner that allows complete and consistent discussions of their performance and potential impacts. They do this by identifying the technologies; presenting their general development status; noting the expected range of NO{sub x} emissions; showing projected costs for each system; describing the various technology, fuel and boiler design factors that contribute to the large span of these ranges; and indicating power plant impacts that may be experienced when applying these controls. These documents are not detailed technical prescriptions for supporting designs or technology selections. All three technology papers focus on retrofit controls, since this is the NO{sub x} compliance issue of greatest urgency to utilities at this time.

  10. 76 FR 77206 - Citric Acid and Certain Citrate Salts From the People's Republic of China: Final Results of...

    Science.gov (United States)

    2011-12-12

    ... provision of sulfuric acid for less than adequate remuneration (``LTAR'') for both RZBC and Yixing, and... requested. Failure to comply with the regulations and the terms of an APO is a sanctionable violation. We...

  11. Do circulating tumor cells, exosomes, and circulating tumor nucleic acids have clinical utility? A report of the association for molecular pathology.

    Science.gov (United States)

    Gold, Bert; Cankovic, Milena; Furtado, Larissa V; Meier, Frederick; Gocke, Christopher D

    2015-05-01

    Diagnosing and screening for tumors through noninvasive means represent an important paradigm shift in precision medicine. In contrast to tissue biopsy, detection of circulating tumor cells (CTCs) and circulating tumor nucleic acids provides a minimally invasive method for predictive and prognostic marker detection. This allows early and serial assessment of metastatic disease, including follow-up during remission, characterization of treatment effects, and clonal evolution. Isolation and characterization of CTCs and circulating tumor DNA (ctDNA) are likely to improve cancer diagnosis, treatment, and minimal residual disease monitoring. However, more trials are required to validate the clinical utility of precise molecular markers for a variety of tumor types. This review focuses on the clinical utility of CTCs and ctDNA testing in patients with solid tumors, including somatic and epigenetic alterations that can be detected. A comparison of methods used to isolate and detect CTCs and some of the intricacies of the characterization of the ctDNA are also provided.

  12. Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors.

    Science.gov (United States)

    Jiang, Jun Fang; Qiao, Juan; Mu, Xiao Yu; Moon, Myeong Hee; Qi, Li

    2017-04-01

    In this work, a unique D-amino acid oxidase reactor for enhanced enzymolysis efficiency is presented. A kind of magnetic polymer matrices, composed of iron oxide nanoparticles and porous polymer membrane (poly styrene-co-maleic anhydride), was prepared. With covalent bonding D-Amino acid oxidase on the surface of the matrices and characterization of scanning electron microscope and vibrating sample magnetometer, it demonstrated that the membrane enzyme reactor was successfully constructed. The enzymolysis efficiency of the enzyme reactor was evaluated and the apparent Michaelis-Menten constants of D-Amino acid oxidase were determined (Km was 1.10mM, Vmax was 23.8mMmin(-1)) by a chiral ligand exchange capillary electrophoresis protocol with methionine as the substrate. The results indicated that the enzyme reactor could exhibit good stability and excellent reusability. Importantly, because the enzyme and the substrate could be confined into the pores of the matrices, the enzyme reactor displayed the improved enzymolysis efficiency due to the confinement effect. Further, the prepared enzyme reactor was applied for D-Amino acid oxidase inhibitors screening. It has displayed that the proposed protocol could pave a new way for fabrication of novel porous polymer membrane based enzyme reactors to screen enzyme inhibitors.

  13. Zinc, magnesium, and calcium ion supplementation confers tolerance to acetic acid stress in industrial Saccharomyces cerevisiae utilizing xylose.

    Science.gov (United States)

    Ismail, Ku Syahidah Ku; Sakamoto, Takatoshi; Hasunuma, Tomohisa; Zhao, Xin-Qing; Kondo, Akihiko

    2014-12-01

    Lignocellulosic biomass is a potential substrate for ethanol production. However, pretreatment of lignocellulosic materials produces inhibitory compounds such as acetic acid, which negatively affect ethanol production by Saccharomyces cerevisiae. Supplementation of the medium with three metal ions (Zn(2+) , Mg(2+) , and Ca(2+) ) increased the tolerance of S. cerevisiae toward acetic acid compared to the absence of the ions. Ethanol production from xylose was most improved (by 34%) when the medium was supplemented with 2 mM Ca(2+) , followed by supplementation with 3.5 mM Mg(2+) (29% improvement), and 180 μM Zn(2+) (26% improvement). Higher ethanol production was linked to high cell viability in the presence of metal ions. Comparative transcriptomics between the supplemented cultures and the control suggested that improved cell viability resulted from the induction of genes controlling the cell wall and membrane. Only one gene, FIT2, was found to be up-regulated in common between the three metal ions. Also up-regulation of HXT1 and TKL1 might enhance xylose consumption in the presence of acetic acid. Thus, the addition of ionic nutrients is a simple and cost-effective method to improve the acetic acid tolerance of S. cerevisiae.

  14. Utilizing Unnatural Amino Acids to Illustrate Protein Structure-Function Relationships: An Experiment Designed for an Undergraduate Biochemistry Laboratory

    Science.gov (United States)

    Maza, Johnathan C.; Villa, Jordan K.; Landino, Lisa M.; Young, Douglas D

    2016-01-01

    The site-specific introduction of unnatural amino acids (UAAs) has been demonstrated to be a useful tool in protein engineering. Moreover, the incorporation of a UAA into a protein has become feasible with the increased commercial availability of UAAs and robust expression plasmids. In addition to the ease of incorporation, the concepts utilized…

  15. Pearl millet utilization in ccommercial laying hen diets formulated on a total or digestible amino acid basis

    Directory of Open Access Journals (Sweden)

    RS Filardi

    2005-06-01

    Full Text Available An experiment was carried out to evaluate the effect of replacing corn with pearl millet in commercial layer diets, formulated according to the minimal requirements for total and digestible amino acids. Two hundred and forty Lohmann LSL laying hens with 25 weeks of age were distributed in a completely randomized experimental design according to a 2 x 5 factorial arrangement with 3 replicates of 8 birds. Feed was formulated on two amino acid basis (total or digestible according to Rostagno et al. (2000 and there were five pearl millet inclusion levels (0%, 25%, 50%, 75%, and 100%. Performance and egg quality were evaluated during five periods of 21 days.At the end of each period, feed intake, egg production, egg weight and feed conversion were evaluated. In the last three days of each period, the following egg quality parameters were evaluated: Haugh Unit, yolk pigmentation index, egg specific weight, shell percentage and shell thickness. Digestible amino acid requirements resulted in decreased feed intake (p<0.01 and increased production costs per mass of eggs (kg or per dozen eggs (p<0.01 compared to total amino acid requirements. There was a linear reduction in feed intake, egg production, egg weight and yolk pigmentation index with increasing inclusion levels of pearl millet. Therefore, increasing levels of replacement of corn by pearl millet affected bird performance negatively. Besides, production costs were higher with increasing pearl millet levels.

  16. Uptake and utilization of dissolved free amino acids by the brittlestar Microphiopholis gracillima (Say, 1852) (Echinodermata: Ophiuroidea) during disc regeneration

    Energy Technology Data Exchange (ETDEWEB)

    Clements, L.A.J.

    1988-01-01

    Dissolved organic material (DOM) may be especially important to marine organisms unable to feed due to lack of a digestive system, injury or autotomy. Disc autotomy by the brittlestar Microphiopholis gracillima includes loss of the gut and gonads, and precludes ingestion of particulate food by the animal until gut regeneration is complete. The influence of DOM on the rate of disc regeneration by autotomized M. gracillima was tested by incubating animals in artificial seawater containing different concentrations of amino acids. Rates of uptake of amino acids and excretion of amino acids, ammonia and urea by intact and regenerating M. gracillima individuals were quantified using high performance liquid chromatography. Uptake and catabolism of leucine and glycine were examined using radioisotopic techniques. Both intact and regenerating M. gracillima increased their uptake of {sup 14}C-glycine with starvation, but rates of uptake were higher for intact animals. Catabolism of leucine and glycine, as evidenced by release of labeled CO{sub 2}, was highest among intact animals indicating that the amino acids were being used as energy sources during starvation. However, regenerating animals catabolized less than 2% of the labeled glycine acquired from seawater.

  17. Development and initial evaluation of a novel method for assessing tissue-specific plasma free fatty acid utilization in vivo using (R)-2-bromopalmitate tracer.

    Science.gov (United States)

    Oakes, N D; Kjellstedt, A; Forsberg, G B; Clementz, T; Camejo, G; Furler, S M; Kraegen, E W; Olwegård-Halvarsson, M; Jenkins, A B; Ljung, B

    1999-06-01

    We describe a method for assessing tissue-specific plasma free fatty acid (FFA) utilization in vivo using a non-beta-oxidizable FFA analog, [9,10-3H]-(R)-2-bromopalmitate (3H-R-BrP). Ideally 3H-R-BrP would be transported in plasma, taken up by tissues and activated by the enzyme acyl-CoA synthetase (ACS) like native FFA, but then 3H-labeled metabolites would be trapped. In vitro we found that 2-bromopalmitate and palmitate compete equivalently for the same ligand binding sites on albumin and intestinal fatty acid binding protein, and activation by ACS was stereoselective for the R-isomer. In vivo, oxidative and non-oxidative FFA metabolism was assessed in anesthetized Wistar rats by infusing, over 4 min, a mixture of 3H-R-BrP and [U-14C] palmitate (14C-palmitate). Indices of total FFA utilization (R*f) and incorporation into storage products (Rfs') were defined, based on tissue concentrations of 3H and 14C, respectively, 16 min after the start of tracer infusion. R*f, but not Rfs', was substantially increased in contracting (sciatic nerve stimulated) hindlimb muscles compared with contralateral non-contracting muscles. The contraction-induced increases in R*f were completely prevented by blockade of beta-oxidation with etomoxir. These results verify that 3H-R-BrP traces local total FFA utilization, including oxidative and non-oxidative metabolism. Separate estimates of the rates of loss of 3H activity indicated effective 3H metabolite retention in most tissues over a 16-min period, but appeared less effective in liver and heart. In conclusion, simultaneous use of 3H-R-BrP and [14C]palmitate tracers provides a new useful tool for in vivo studies of tissue-specific FFA transport, utilization and metabolic fate, especially in skeletal muscle and adipose tissue.

  18. Degradation of cellulosic biomass and its subsequent utilization for the production of chemical feedstocks. Final report, February 1, 1978-January 31, 1979

    Energy Technology Data Exchange (ETDEWEB)

    None

    1979-01-01

    This is a coordinated program to effect the microbiological degradation of cellulosic biomasses and will focus on the use of anaerobic microorganisms which possess cellulolytic enzyme. The studies will attempt to increase the enzyme levels through genetics, mutation and strain selection. In addition, the direct conversion from cellulosic biomasses to liquid fuel (ethanol) and/or soluble sugars by the cellulolytic, anaerobic organism is also within the scope of this program. Process and engineering scale-up, along with economic analyses, will be performed throughout the course of the program. The second area of our major effort is devoted to the production of chemical feedstocks. In particular, three fermentations have been identified for exploration. These are: acrylic acid, acetone/butanol and acetic acid. The main efforts in these fermentations will address means for the reduction of the cost of manufacturing for these large volume chemicals.

  19. Impact of improving dietary amino acid balance for lactating sows on efficiency of dietary amino acid utilization and transcript abundance of genes encoding lysine transporters in mammary tissue.

    Science.gov (United States)

    Huber, L; de Lange, C F M; Ernst, C W; Krogh, U; Trottier, N L

    2016-11-01

    Lactating multiparous Yorkshire sows ( = 64) were used in 2 experiments to test the hypothesis that reducing dietary CP intake and improving AA balance through crystalline AA (CAA) supplementation improves apparent dietary AA utilization efficiency for milk production and increases transcript abundance of genes encoding Lys transporter proteins in mammary tissue. In Exp. 1, 40 sows were assigned to 1 of 4 diets: 1) high CP (HCP; 16.0% CP, as-fed basis; analyzed concentration), 2) medium-high CP (MHCP; 15.7% CP), 3) medium-low CP (MLCP; 14.3% CP), and 4) low CP (LCP; 13.2% CP). The HCP diet was formulated using soybean meal and corn as the only Lys sources. The reduced-CP diets contained CAA to meet estimated requirements for essential AA that became progressively limiting with reduction in CP concentration, that is, Lys, Ile, Met + Cys, Thr, Trp, and Val. Dietary standardized ileal digestible (SID) Lys concentration was 80% of the estimated requirement. In Exp. 2, 24 sows were assigned to the HCP or LCP diets. In Exp. 1, blood samples were postprandially collected 15 h on d 3, 7, 14, and 18 of lactation and utilization efficiency of dietary AA for milk production was calculated during early (d 3 to 7) and peak (d 14 to 18) lactation. Efficiency values were estimated from daily SID AA intakes and milk AA yield, with corrections for maternal AA requirement for maintenance and AA contribution from body protein losses. In Exp. 2, mammary tissue was biopsied on d 4 and 14 of lactation to determine the mRNA abundance of genes encoding Lys transporter proteins. In peak lactation, Lys, Thr, Trp, and Val utilization efficiency increased with decreasing dietary CP (linear for Trp and Val, sows fed the MHCP diet vs. sows fed the HCP diet for Lys and Thr, sows fed the LCP and HCP diets. Feeding lactating sows low-CP diets supplemented with CAA increases the efficiency of utilizing dietary Lys, Thr, Trp, and Val for milk protein production but is unrelated to abundance in m

  20. So/sub 2/ reduction in non-utility combustion sources: technical and economic comparison of alternatives. Final task report, May 1974--Sep 1975

    Energy Technology Data Exchange (ETDEWEB)

    Choi, P.S.K.; Kropp, E.L.; Ballantyne, W.E.; Anastas, M.Y.; Putnam, A.A.

    1975-10-01

    The report gives results of an analysis of non-utility combustion (NUC) sources for various size classes and fuel types with respect to the significance of SO/sub 2/ emissions. Technical and economic comparisons of various SO/sub 2/ control alternatives were made for the important size classes and fuel types. Categories of alternatives included are: physical cleaning of coal, coal gasification, coal liquefaction, fluidized-bed combustion of coal, and flue gas desulfurization. For small size classes of NUC sources, applicabilities of package sorption systems are reviewed. (GRA)

  1. Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum

    Energy Technology Data Exchange (ETDEWEB)

    Svetlitshnyi, V.; Wiegel, J. [Univ. of Georgia, Athens, GA (United States); Rainey, F. [German Collection of Microorganisms and Cell Cultures, Braunschweig (Germany)

    1996-10-01

    Three strains of an anaerobic thermophilic organoheterotrophic lipolytic alkalitolerant bacterium, Thermosyntropha lipolytica gen. nov., sp. nov. (type strain JW/VS-264{sup T}; DSM 11003) were isolated from alkaline hot springs of Lake Bogoria (Kenya). The cells were nonmotile, non-spore forming, straight or slightly curved rods. At 60{degrees}C, the pH range for growth determined at 25{degrees}C [pH{sup 25{degrees}C}] was 7.15 to 9.5, with an optimum between 8.1 and 8.9 (pH{sup 60{degrees}C} of 7.6 and 8.1). At a pH{sup 25{degrees}C} of 8.5 temperature range for growth was from 52 to 70{degrees}C, with an optimum between 60 and 66{degrees}C. The shortest doubling time was around 1 h. In pure culture the bacterium grew in a mineral base medium supplemented with yeast extract, tryptone, Casamino Acids, betaine, and crotonate as carbon sources, producing acetate as a major product and constitutively a lipase. During growth in the presence of olive oil, free long-chain fatty acids were accumulated in the medium but the pure culture syntrophic coculture (Methanobacterium strain JW/VS-M29) the lipolytic bacteria grew on triacylglycerols and linear saturated and unsaturated fatty acids with 4 to 18 carbon atoms, but glycerol was not utilized. Fatty acids with even numbers of carbon atoms were degraded to acetate and methane, while from odd-numbered fatty acids 1 mol of propionate per mol of fatty acid was additionally formed. 16S rDNA sequence analysis identified Syntrophospora and Syntrophomonas spp. as closest phylogenetic neighbors.

  2. Using tobacco waste extract in pre-culture medium to improve xylose utilization for l-lactic acid production from cellulosic waste by Rhizopus oryzae.

    Science.gov (United States)

    Zheng, Yuxi; Wang, Yuanliang; Zhang, Jianrong; Pan, Jun

    2016-10-01

    The aim of this work was to study the high-titer l-lactic acid production from cellulosic waste using Rhizopus oryzae. The tobacco waste water-extract (TWE) added with 5g/L glucose and 0.1g/L vitamin C was optimized as pre-culture medium for R. oryzae. Results found that compared to traditional pre-culture medium, it improved xylose consumption rate up to 2.12-fold and enhanced l-lactic acid yield up to 1.73-fold. The highest l-lactic acid concentration achieved was 173.5g/L, corresponding to volumetric productivity of 1.45g/Lh and yield of 0.860g/g total reducing sugar in fed-batch fermentation. This process achieves efficient production of polymer-grade l-lactic acid from cellulosic feedstocks, lowers the cost of fungal cell pre-culture and provides a novel way for re-utilization of tobacco waste.

  3. Direct utilization of geothermal resources at Warm Springs State Hospital, Warm Springs, Montana. Final report, January 31, 1979-June 30, 1983

    Energy Technology Data Exchange (ETDEWEB)

    1984-01-01

    Several decades ago the water from a natural hot spring was piped to the Warm Springs State Hospital barn and greenhouse and eventually into the domestic water supply for showers. The Montana Department of Natural Resources and Conservation (DNRC) funded a feasibility study on potential development of the geothermal resource from monies originating from coal severence taxes. The results of the feasibility study were subsequently utilized in obtaining a $721,122 award from the Department of Energy Program Opportunity Notice (PON) program to identify and develop the geothermal resource at Warm Springs. The study included environmental and legal considerations, geophysical surveys, and the subsequent development of the resource. The well produces 60 to 64 gpm of 154/sup 0/F geothermal water which is utilized in a heat exchanger to heat domestic water. The system became fully operational on January 13, 1983 and the calculated yearly energy savings represent approximately 17.6 million cubic feet of natural gas which is equivalent to $77,000, based on current prices.

  4. Simultaneous utilization of cellobiose, xylose, and acetic acid from lignocellulosic biomass for biofuel production by an engineered yeast platform.

    Science.gov (United States)

    Wei, Na; Oh, Eun Joong; Million, Gyver; Cate, Jamie H D; Jin, Yong-Su

    2015-06-19

    The inability of fermenting microorganisms to use mixed carbon components derived from lignocellulosic biomass is a major technical barrier that hinders the development of economically viable cellulosic biofuel production. In this study, we integrated the fermentation pathways of both hexose and pentose sugars and an acetic acid reduction pathway into one Saccharomyces cerevisiae strain for the first time using synthetic biology and metabolic engineering approaches. The engineered strain coutilized cellobiose, xylose, and acetic acid to produce ethanol with a substantially higher yield and productivity than the control strains, and the results showed the unique synergistic effects of pathway coexpression. The mixed substrate coutilization strategy is important for making complete and efficient use of cellulosic carbon and will contribute to the development of consolidated bioprocessing for cellulosic biofuel. The study also presents an innovative metabolic engineering approach whereby multiple substrate consumption pathways can be integrated in a synergistic way for enhanced bioconversion.

  5. Effects of dietary methionine on feed utilization, plasma amino acid profiles and gene expression in rainbow trout (Oncorhynchus mykiss)

    DEFF Research Database (Denmark)

    Rolland, Marine

    (EAA) compared to dietary requirements. Supplementation with amino acids in crystalline from (CAA) is a common practice to balance the dietary amino acid profile to achieve high growth performances. However, complete substitution of fish meal using plant proteins and CAAs often results in poorer growth...... are presented in Paper I and show that the protein source itself (fish meal or plant based) does not affect the plasma EAA profiles, but rather that plasma EAA levels reflect the dietary level. Supplementation with histidine, lysine and threonine in crystalline form to a plant based diet was, on the other hand...... as an apparent “accumulation” in the plasma, compared to fish fed similar dietary level but in protein bound form. The study further showed that the nitrogen excretion resulting from feeding an AA deficient diet was higher than for the fish meal control diet. Supplementation of the plant meal diet...

  6. Ferulic acid-coupled chitosan: thermal stability and utilization as an antioxidant for biodegradable active packaging film.

    Science.gov (United States)

    Woranuch, Sarekha; Yoksan, Rangrong; Akashi, Mitsuru

    2015-01-22

    The aim of the present research was to study the thermal stability of ferulic acid after coupling onto chitosan, and the possibility of using ferulic acid-coupled chitosan (FA-CTS) as an antioxidant for biodegradable active packaging film. FA-CTS was incorporated into biodegradable film via a two-step process, i.e. compounding extrusion at temperatures up to 150°C followed by blown film extrusion at temperatures up to 175°C. Although incorporation of FA-CTS with a content of 0.02-0.16% (w/w) caused decreased water vapor barrier property and reduced extensibility, the biodegradable films possessed improved oxygen barrier property and antioxidant activity. Radical scavenging activity and reducing power of film containing FA-CTS were higher than those of film containing naked ferulic acid, by about 254% and 94%, respectively. Tensile strength and rigidity of the films were not significantly affected by the addition of FA-CTS with a content of 0.02-0.08% (w/w). The above results suggested that FA-CTS could potentially be used as an antioxidant for active packaging film.

  7. 77 FR 72323 - Citric Acid and Certain Citrate Salts From the People's Republic of China: Final Results of...

    Science.gov (United States)

    2012-12-05

    ..., the cash deposit rates that will be applied to companies covered by this order, but not examined in... submitted has not resulted in a change to the net subsidy rate for RZBC Co., Ltd., RZBC Juxian Co., Ltd... RZBC). The final net subsidy rate for the RZBC Companies is listed below in the section...

  8. Medical Devices; Immunology and Microbiology Devices; Classification of Gastrointestinal Microorganism Multiplex Nucleic Acid-Based Assay. Final order.

    Science.gov (United States)

    2015-11-02

    The Food and Drug Administration (FDA) is classifying a gastrointestinal microorganism multiplex nucleic acid-based assay into class II (special controls). The Agency is classifying the device into class II (special controls) in order to provide a reasonable assurance of safety and effectiveness of the device.

  9. [Origin of Lewis acidity in solid materials]. DOE Final Report for Grant DE-FG02-90ER14130

    Energy Technology Data Exchange (ETDEWEB)

    Fripiat, J. J.

    2001-04-11

    The aim of the research undertaken within the framework of this DOE grant was to further understanding of the origin of the Lewis acidity in solid materials. The study centered around aluminas and alumino-silicates. The main tools for investigation of this phenomenon were high-resolution solid state {sub 27}Al NMR, complemented by EPR and the chemical determination of catalytic activity.

  10. Oxygen and silicon stable isotopes of diatom silica. Reconstructing changes in surface water hydrography and silicic acid utilization in the late Pleistocene subarctic Pacific

    Energy Technology Data Exchange (ETDEWEB)

    Maier, Edith

    2014-03-05

    Deglacial variations in upper ocean nutrient dynamics and stratification in high latitudes, as well as associated changes in thermohaline overturning circulation, are thought to have played a key role in changing atmospheric CO{sub 2} concentrations. This thesis examines the relationship between past changes in subarctic Pacific upper ocean stratification and nutrient (silicic acid) utilization, using oxygen and silicon stable isotopes of diatom silica, for the first time at millennial-scale resolution and analyzed with a new and efficient instrumentation set-up. The isotopic data, presented in three manuscripts, show a consistent picture of millennial-scale variability in upper ocean stratification and silicic acid utilization during the last ∝50 ka BP, e.g. indicating that the subarctic Pacific was a source region for atmospheric CO{sub 2} during the last deglaciation (late Heinrich Stadial 1 and the Boelling/Alleroed). The presented results demonstrate the high potential of combined diatom oxygen and silicon stable isotope analysis especially for, but not restricted to, marine regions characterized by a low biogenic carbonate content like the subarctic Pacific and the Southern Ocean.

  11. Adipocyte Accumulation of Long-Chain Fatty Acids in Obesity is Multifactorial, Resulting from Increased Fatty Acid Uptake and Decreased Activity of Genes Involved in Fat Utilization

    Science.gov (United States)

    Walewski, José L.; Ge, Fengxia; Gagner, Michel; Inabnet, William B.; Pomp, Alfons; Branch, Andrea D.

    2010-01-01

    Background The obesity epidemic causes significant morbidity and mortality. Knowledge of cellular function and gene expression in obese adipose tissue will yield insights into obesity pathogenesis and suggest therapeutic targets. The aim of this work is to study the processes determining fat accumulation in adipose tissue from obese patients. Methods Omental fat was collected from two cohorts of obese bariatric surgery patients and sex-matched normal-weight donors. Isolated adipocytes were compared for cell size, volume, and long-chain fatty acid (LCFA) uptake. Omental fat RNAs were screened by 10K microarray (cohort 1: three obese, three normal) or Whole Genome microarray (cohort 2: seven obese, four normal). Statistical differences in gene and pathway expression were identified in cohort 1 using the GeneSifter Software (Geospiza) with key results confirmed in cohort 2 samples by microarray, quantitative real-time polymerase chain reaction, and pathway analysis. Results Obese omental adipocytes had increased surface area, volume, and Vmax for saturable LCFA uptake. Dodecenoyl-coenzyme A delta isomerase, central to LCFA metabolism, was approximately 1.6-fold underexpressed in obese fat in cohorts 1 and 2. Additionally, the Kyoto Encyclopedia of Genes and Genomics pathway analysis identified oxidative phosphorylation and fatty acid metabolism pathways as having coordinate, nonrandom down-regulation of gene expression in both cohorts. Conclusions In obese omental fat, saturable adipocyte LCFA uptake was greater than in controls, and expression of key genes involved in lipolysis, β-oxidation, and metabolism of fatty acids was reduced. Thus, both increased uptake and reduced metabolism of LCFAs contribute to the accumulation of LCFAs in obese adipocytes. PMID:19866242

  12. The Acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular Utilization

    DEFF Research Database (Denmark)

    Færgeman, Nils J.; Black, P N; Zhao, X D

    2001-01-01

    Exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to metabolic utilization. In the yeast Saccharomyces cerevisiae, the activation of these compounds prior to metabolic utilization proceeds through the fatty acyl-CoA synthetases Faa1p and Faa4p. Faa1p or Faa4p are esse...

  13. The PPAR α / γ Agonist, Tesaglitazar, Improves Insulin Mediated Switching of Tissue Glucose and Free Fatty Acid Utilization In Vivo in the Obese Zucker Rat.

    Science.gov (United States)

    Wallenius, Kristina; Kjellstedt, Ann; Thalén, Pia; Löfgren, Lars; Oakes, Nicholas D

    2013-01-01

    lean controls, obese controls, and obese rats treated with the dual peroxisome proliferator activated receptor (PPAR) α/γ agonist, tesaglitazar, 3  μ mol/kg/day for 3 weeks. Whole body glucose disposal rate (R d ) and hepatic glucose output (HGO) were assessed under basal fasting and hyperinsulinemic isoglycemic clamp conditions using [3,(3)H]glucose. Indices of tissue specific glucose utilization (R g ') were measured at basal, physiological, and supraphysiological levels of insulinemia using 2-deoxy-D-[2,6-(3)H]glucose. Finally, whole body and tissue specific FFA and glucose utilization and metabolic fate were evaluated under basal and hyperinsulinemic conditions using a combination of [U-(13)C]glucose, 2-deoxy-D-[U-(14)C]glucose, [U-(14)C]palmitate, and [9,10-(3)H]-(R)-bromopalmitate. Tesaglitazar improved whole body insulin action by greater suppression of HGO and stimulation of R d compared to obese controls. This involved increased insulin stimulation of R g ' in fat and skeletal muscle as well as increased glycogen synthesis. Tesaglitazar dramatically improved insulin mediated suppression of plasma FFA level, whole body turnover (R fa ), and muscle, liver, and fat utilization. At basal insulin levels, tesaglitazar failed to lower HGO or R fa compared to obese controls. In conclusion, the results demonstrate that tesaglitazar has a remarkable ability to improve insulin mediated control of glucose and FFA fluxes in obese Zucker rats.

  14. Direct utilization of geothermal heat in cascade application to aquaculture and greenhouse systems at Navarro College. Final report, March 1, 1979-September 30, 1984

    Energy Technology Data Exchange (ETDEWEB)

    Smith, K.

    1984-09-01

    This final report documents the Navarro College geothermal use project, which is one of nineteen direct-use geothermal projects funded principally by DOE. The six-year project encompassed a broad range of technical, institutional, and economic activities including: resource and environmental assessment; well drilling and completion; system design, construction, and monitoring; economic analysis; and public awareness programs. Some of the project conclusions are that: (1) the 130/sup 0/F Central Texas geothermal resource can support additional geothermal development; (2) private sector economic incentives currently exist which encourage commercial development of this geothermal resource; (3) potential uses for this geothermal resource include water and space heating, aquacultural and agricultural heating uses, and fruit and vegetable dehydration; (4) high maintenance costs arising from the geofluids' scaling and corrosion characteristics can be avoided through proper analysis and design.

  15. Direct utilization of geothermal heat in cascade application to aquaculture and greenhouse systems at Navarro College. Final report, March 1, 1979-September 30, 1984

    Energy Technology Data Exchange (ETDEWEB)

    Smith, K.

    1984-09-01

    This final report documents the Navarro College geothermal use project, which is one of nineteen direct-use geothermal projects funded principally by DOE. The six-year project encompassed a broad range of technical, institutional, and economic activities including: resource and environmental assessment; well drilling and completion; system design, construction, and monitoring; economic analysis; and public awareness programs. Some of the project conclusions are that: (1) the 130/sup 0/F Central Texas geothermal resource can support additional geothermal development; (2) private sector economic incentives currently exist which encourage commercial development of this geothermal resource; (3) potential uses for this geothermal resource include water and space heating, aquacultural and agricultural heating uses, and fruit and vegetable dehydration; (4) high maintenance costs arising from the geofluids' scaling and corrosion characteristics can be avoided through proper analysis and design.

  16. Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12.

    Science.gov (United States)

    White-Ziegler, Christine A; Malhowski, Amy J; Young, Sarah

    2007-08-01

    Using DNA microarrays, we identified 126 genes in Escherichia coli K-12 whose expression is increased at human body temperature (37 degrees C) compared to growth at 23 degrees C. Genes involved in the uptake and utilization of amino acids, carbohydrates, and iron dominated the list, supporting a model in which temperature serves as a host cue to increase expression of bacterial genes needed for growth. Using quantitative real-time PCR, we investigated the thermoregulatory response for representative genes in each of these three categories (hisJ, cysP, srlE, garP, fes, and cirA), along with the fimbrial gene papB. Increased expression at 37 degrees C compared to 23 degrees C was retained in both exponential and stationary phases for all of the genes and in most of the various media tested, supporting the relative importance of this cue in adapting to changing environments. Because iron acquisition is important for both growth and virulence, we analyzed the regulation of the iron utilization genes cirA and fes and found that growth in iron-depleted medium abrogated the thermoregulatory effect, with high-level expression at both temperatures, contrasting with papB thermoregulation, which was not greatly altered by limiting iron levels. A positive role for the environmental regulator H-NS was found for fes, cirA, hisJ, and srlE transcription, whereas it had a primarily negative effect on cysP and garP expression. Together, these studies indicate that temperature is a broadly used cue for regulating gene expression in E. coli and that H-NS regulates iron, carbohydrate, and amino acid utilization gene expression.

  17. Amino acid efflux by asexual blood-stage Plasmodium falciparum and its utility in interrogating the kinetics of hemoglobin endocytosis and catabolism in vivo.

    Science.gov (United States)

    Dalal, Seema; Klemba, Michael

    2015-06-01

    The endocytosis and catabolism of large quantities of host cell hemoglobin is a hallmark of the intraerythrocytic asexual stage of the human malaria parasite Plasmodium falciparum. It is known that the parasite's production of amino acids from hemoglobin far exceeds its metabolic needs. Here, we show that P. falciparum effluxes large quantities of certain non-polar (Ala, Leu, Val, Pro, Phe, Gly) and polar (Ser, Thr, His) amino acids to the external medium. That these amino acids originate from hemoglobin catabolism is indicated by the strong correlation between individual amino acid efflux rates and their abundances in hemoglobin, and the ability of the food vacuole falcipain inhibitor E-64d to greatly suppress efflux rates. We then developed a rapid, sensitive and precise method for quantifying flux through the hemoglobin endocytic-catabolic pathway that is based on leucine efflux. Optimization of the method involved the generation of a novel amino acid-restricted RPMI formulation as well as the validation of D-norvaline as an internal standard. The utility of this method was demonstrated by characterizing the effects of the phosphatidylinositol-3-kinase inhibitors wortmannin and dihydroartemisinin on the kinetics of Leu efflux. Both compounds rapidly inhibited Leu efflux, which is consistent with a role for phosphtidylinositol-3-phosphate production in the delivery of hemoglobin to the food vacuole; however, wortmannin inhibition was transient, which was likely due to the instability of this compound in culture medium. The simplicity, convenience and non-invasive nature of the Leu efflux assay described here makes it ideal for characterizing the in vivo kinetics of hemoglobin endocytosis and catabolism, for inhibitor target validation studies, and for medium-throughput screens to identify novel inhibitors of cytostomal endocytosis.

  18. [Protein and energy value of spiruline blue algae supplemented by amino acids: digestive and metabolic utilization by the growing rat].

    Science.gov (United States)

    Vermorel, M; Toullec, G; Dumond, D; Pion, R

    1975-01-01

    Protein and energy value of 6 samples of "Spirulina" was studied on growing rats in 1972 and 1973. Sample RL 1(Spirulina platensis, originating from Tchad) was grown in artifical conditions in a laboratory. Others samples (Spirulina maxima) were grown in the solar evaporator near Mexico, washed and dried either on heated rollers (MR8, MR13) or by spraying (MA 7, MA10). Sample MA10 D corresponds to sample MA10, bleached by ethanol plus acetone (Baron, 1975). Each Spirulina sample was the only protein source of balanced, starch diets. The diets were supplemented in essential amino acids (E.A.A.) according to the requirements of growing rats (table 1). The ratios [(digestible nitrogen/metabolisable energy (EM] of the Spirulina diets were similar to that of the control diets containing herring meal. The diets were fed to groups of 15 to 17 growing rats. Energy and nitrogen balances were established by the comparative slaughter technique. Blood and muscle samples were taken at slaughter for the determination of free amino acids levels.

  19. Compressed air energy storage: preliminary design and site development program in an aquifer. Final draft, Task 1: establish facility design criteria and utility benefits

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-10-01

    Compressed air energy storage (CAES) has been identified as one of the principal new energy storage technologies worthy of further research and development. The CAES system stores mechanical energy in the form of compressed air during off-peak hours, using power supplied by a large, high-efficiency baseload power plant. At times of high electrical demand, the compressed air is drawn from storage and is heated in a combustor by the burning of fuel oil, after which the air is expanded in a turbine. In this manner, essentially all of the turbine output can be applied to the generation of electricity, unlike a conventional gas turbine which expends approximately two-thirds of the turbine shaft power in driving the air compressor. The separation of the compression and generation modes in the CAES system results in increased net generation and greater premium fuel economy. The use of CAES systems to meet the utilities' high electrical demand requirements is particularly attractive in view of the reduced availability of premium fuels such as oil and natural gas. This volume documents the Task 1 work performed in establishing facility design criteria for a CAES system with aquifer storage. Information is included on: determination of initial design bases; preliminary analysis of the CAES system; development of data for site-specific analysis of the CAES system; detailed analysis of the CAES system for three selected heat cycles; CAES power plant design; and an economic analysis of CAES.

  20. Final Technical Report for USDOE Grant No. DE-FG02-96ER14675 Supermolecular Photosynthetic Arrays: Construction, Characterization, Exploration and Utilization

    Energy Technology Data Exchange (ETDEWEB)

    Norris, James

    2009-12-13

    Biological processes provide paradigms for the development of solar energy devices of practical utility. In nature, the light harvesting -complexes (LHCs) are not chemically active. However, with unnatural chemical oxidation by potassium ferricyanide, cation free radicals of bacteriochlorophyll (BChl) can be formed in the light harvesting complex 1 (LH1) of Rhodobacter sphaeroides. Based on EPR studies, the site of the BChl{sup +} cations move rather freely about the LH1 complex as in a molecular wire. These molecular wires function in the frozen, solid state. This work seeks to understand better how nature controls electron transfer in some of its molecular wires. To investigate the nature of electron-hole transfer we have e.onducted both electron paramagnetic resonance (EPR) and electron nuclear double resonance (ENDOR) experiments on oxidized LH1 complexes. Progress has been achieved in two main areas: EPR studies of the role of ferricyanide in the molecular wire nature of oxidized LH1; and ENDOR studies of oxidized LH1 at 80K.

  1. Technological assessment of demonstration plants for environmentally compatible processing and utilization of liquid manure. Final report; Technologische Bewertung von Demonstrationsanlagen zur umweltvertraeglichen Guelleaufbereitung und -verwertung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Weiland, P.; Huettner, A.

    1997-09-01

    The aim of the BMBF-sponsored research programme ``Environmentally compatible liquid manure processing and utilization`` was to develop and test technical processing methods permitting environmentally friendly storage, application and harnessing of liquid manure. For practical testing and optimization, five demonstration plants were erected, which achieve partial or total cleaning of liquid manure by applying different techniques. All processes had the objective of eliminating from the liquid manure nutrients, organic constituents and odourous components, recovering the nutrients in the form of transportable products, and harnessing the liquid manure for biogas generation. (orig.) [Deutsch] Ziel des BMBF-Foerderschwerpunktes `Umweltvertraegliche Guelleaufbereitung und -verwertung` war es, technische Aufbereitungsverfahren zu entwickeln und zu erproben, die eine umweltvertraegliche Lagerung, Ausbringung und Verwertung der Guelle ermoeglichen. Zur Praxiserprobung und Optimierung wurden fuenf Demonstrationsanlagen errichtet, die durch Anwendung unterschiedlicher Verfahren eine Teil- bzw. Totalreinigung der Guelle bewirken. Aufgabe saemtlicher Verfahren war es, die Guelle von Naehrstoffen, organischen Inhaltsstoffen und Geruchsstoffen zu befreien, die Naehrstoffe in Form transportwuerdiger Produkte zurueckzugewinnen und die Guelle durch Erzeugung von Biogas energetisch zu nutzen. (orig.)

  2. Comparative Cytotoxicity and Sperm Motility Using a Computer-Aided Sperm Analysis System (CASA) for Isomers of Phthalic Acid, a Common Final Metabolite of Phthalates.

    Science.gov (United States)

    Kwack, Seung Jun; Lee, Byung-Mu

    2015-01-01

    The general population is exposed to phthalates through consumer products, diet, and medical devices. Phthalic acid (PA) is a common final metabolite of phthalates, and its isomers include isophthalic acid (IPA), terephthalic acid (TPA), and phthalaldehyde (o-phthalic acid, OPA). The purpose of this study was to investigate whether PA and PA isomers exert reproductive toxicity, including altered sperm movement. In vitro cell viability assays were comparatively performed using Sertoli and liver cell lines. In animal experiments, PA or PA isomers (10, 100, or 1000 mg/kg) were administered orally to Sprague-Dawley (SD) rats, and semen samples were analyzed by computer-aided sperm analysis (CASA). PA treatment produced a significant effect on curvilinear velocity (VCL), straight-line velocity (VSL), mean velocity or average path velocity (VAP), amplitude of lateral head displacement (ALH), and frequency of head displacement or beat cross-frequency (BCF), whereas IPA, TPA, and OPA induced no marked effects. In vitro cell viability assays showed that mouse normal testis cells (TM4) and human testis cancer cells (NTERA 2 cl. D1) were more sensitive to PA and OPA than mouse liver normal cells (NCTC clone 1469) and human fetal liver cells (FL 62891). Our study suggests that PA and PA isomers specifically produced significant in vitro and in vivo reproductive toxicity, particularly sperm toxicity and testis cell cytotoxicity. Of the isomers examined, PA appeared to be the most toxic and may serve as a surrogate biomarker for reproductive toxicity following mixed exposure to phthalates.

  3. PERK Utilizes Intrinsic Lipid Kinase Activity To Generate Phosphatidic Acid, Mediate Akt Activation, and Promote Adipocyte Differentiation

    Science.gov (United States)

    Bobrovnikova-Marjon, Ekaterina; Pytel, Dariusz; Riese, Matthew J.; Vaites, Laura Pontano; Singh, Nickpreet; Koretzky, Gary A.; Witze, Eric S.

    2012-01-01

    The endoplasmic reticulum (ER) resident PKR-like kinase (PERK) is necessary for Akt activation in response to ER stress. We demonstrate that PERK harbors intrinsic lipid kinase, favoring diacylglycerol (DAG) as a substrate and generating phosphatidic acid (PA). This activity of PERK correlates with activation of mTOR and phosphorylation of Akt on Ser473. PERK lipid kinase activity is regulated in a phosphatidylinositol 3-kinase (PI3K) p85α-dependent manner. Moreover, PERK activity is essential during adipocyte differentiation. Because PA and Akt regulate many cellular functions, including cellular survival, proliferation, migratory responses, and metabolic adaptation, our findings suggest that PERK has a more extensive role in insulin signaling, insulin resistance, obesity, and tumorigenesis than previously thought. PMID:22493067

  4. PERK utilizes intrinsic lipid kinase activity to generate phosphatidic acid, mediate Akt activation, and promote adipocyte differentiation.

    Science.gov (United States)

    Bobrovnikova-Marjon, Ekaterina; Pytel, Dariusz; Riese, Matthew J; Vaites, Laura Pontano; Singh, Nickpreet; Koretzky, Gary A; Witze, Eric S; Diehl, J Alan

    2012-06-01

    The endoplasmic reticulum (ER) resident PKR-like kinase (PERK) is necessary for Akt activation in response to ER stress. We demonstrate that PERK harbors intrinsic lipid kinase, favoring diacylglycerol (DAG) as a substrate and generating phosphatidic acid (PA). This activity of PERK correlates with activation of mTOR and phosphorylation of Akt on Ser473. PERK lipid kinase activity is regulated in a phosphatidylinositol 3-kinase (PI3K) p85α-dependent manner. Moreover, PERK activity is essential during adipocyte differentiation. Because PA and Akt regulate many cellular functions, including cellular survival, proliferation, migratory responses, and metabolic adaptation, our findings suggest that PERK has a more extensive role in insulin signaling, insulin resistance, obesity, and tumorigenesis than previously thought.

  5. A study of toxic emissions from a coal-fired power plant utilizing an ESP/Wet FGD system. Volume 1, Sampling, results, and special topics: Final report

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-01

    This was one of a group of assessments of toxic emissions from coal-fired power plants, conducted for DOE-PETC in 1993 as mandated by the 1990 Clean Air Act. It is organized into 2 volumes; Volume 1 describes the sampling effort, presents the concentration data on toxic chemicals in several power plant streams, and reports the results of evaluations and calculations. The study involved solid, liquid, and gaseous samples from input, output, and process streams at Coal Creek Station Unit No. 1, Underwood, North Dakota (1100 MW mine-mouth plant burning lignite from the Falkirk mine located adjacent to the plant). This plant had an electrostatic precipitator and a wet scrubber flue gas desulfurization unit. Measurements were conducted on June 21--24, 26, and 27, 1993; chemicals measured were 6 major and 16 trace elements (including Hg, Cr, Cd, Pb, Se, As, Be, Ni), acids and corresponding anions (HCl, HF, chloride, fluoride, phosphate, sulfate), ammonia and cyanide, elemental C, radionuclides, VOCs, semivolatiles (incl. PAH, polychlorinated dioxins, furans), and aldehydes. Volume 2: Appendices includes process data log sheets, field sampling data sheets, uncertainty calculations, and quality assurance results.

  6. Formerly utilized MED/AEC sites Remedial Action Program: radiological survey of the Bayo Canyon, Los Alamos, New Mexico. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Mayfield, D L; Stoker, A K; Ahlquist, A J [comps.

    1979-06-01

    A portion of Bayo Canyon, located in Los Alamos County in north-central New Mexico, was used between 1944 and 1961 as a site for experiments employing conventional high explosives in conjunction with research on nuclear weapons development. Radiochemistry operations conducted at the site resulted in the generation of liquid and solid radioactive wastes, which were disposed into subsurface pits and leaching fields. The site was decommissioned by 1963. The resurvey utilized information from a number of routine and special environmental surveillance studies as well as extensive new instrumental measurements, soil sampling, and radiochemical analyses. Results showed that residual surface contamination due to /sup 90/Sr averaged about 1.4 pCi/g or approximately 3 times the level attributable to worldwide fallout. Surface uranium averaged about 4.9 ..mu..g/g or about 1.5 times the amount naturally present in the volcanic-derived soils of the area. Subsurface contamination associated with the former waste disposal locations is largely confined within a total area of about 10,000 m/sup 2/ and down to depths of about 5 m. Of 378 subsurface samples, fewer than 12% exceeded 13 pCi/g of gross beta activity, which is comparable to the upper range of activities for uncontaminated local soils. Health physics interpretation of the data indicates that the present population of Los Alamos living on mesas adjacent to Bayo Canyon is not receiving any incremental radiation doses due to the residual contamination. Potential future land uses of Bayo Canyon include development of a residential area.

  7. Prédictions moyen infrarouges des acides du lait : résultats finals du projet RobustMilk

    OpenAIRE

    Soyeurt, Hélène; McParland, Sinead; Berry, Donagh; Wall, Eileen; Coffey, Mike; Gengler, Nicolas; Dehareng, Frédéric; Pierre DARDENNE

    2012-01-01

    The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on MIR spectra and FA from multiple countries, production systems, and breeds were used to develop equations to predict milk FA. The calibration set contained 1,776 spectrally different English, Irish, and Belgian milk samples collected for over 6 years. FA were quantified by gas chromatography ...

  8. Carcinogenicity of azo dyes: Acid Black 52 and Yellow 3 in hamsters and rats. Volume 2. Technical report (Final)

    Energy Technology Data Exchange (ETDEWEB)

    Plankenhorn, L.J.

    1983-09-30

    This document is an appendix to a study concerning the carcinogenicity of the azo dyes acid-black-52 and yellow-3 in male and female hamsters and rats and contains individual histopathology studies of both dyes. Histopathological features were reported in tabular form for the skin, mammary gland, muscle, salivary gland, mandibular lymph node, sciatic nerve, thymus, larynx, thyroid, parathyroid, trachea, bronchus, esophagus, adrenal, stomach, duodenum, jejunem, ileum, cecum, colon, rectum, mesenteric lymph node, lung, liver, gallbladder, spleen, pancreas, kidney, heart, urinary bladder, seminal vesicle, prostate, testis, cerebrum, cerebellum, pituitary, sternabrae, femur, bone marrow, and nasal cavity.

  9. Utilization of [14C]phenylalanine derived from arylphorin or free amino acid in Manduca sexta pharate adults

    Science.gov (United States)

    Wu, M.; Tischler, M. E.

    1995-01-01

    The role of arylphorin as a storage protein was studied using 14C-arylphorin. 14C-arylphorin was produced optimally by incubating one-half fat body from Manduca sexta fifth instar larvae at 22 degrees C for 24 h, in 1 ml of medium containing amino acids at 25% of their physiological concentration with [U-14C]-phenylalanine (phe) provided initially without nonlabeled phenylalanine. Nonlabeled phe was provided after 1 h at 16% of its physiological concentration. The specific activity of 14C-arylphorin produced in vitro was 30 times greater than that generated in vivo. Injection of 14C-arylphorin into pharate adults was used to study the distribution of 14C-phe derived from this protein into 14CO2 and tissues for comparison with injection of free 14C-phe during the middle (days 6 to 12 pharate adult) and late (days 12 to 17 pharate adult) stages of adult development. Appearance of 14CO2 from 14C-arylphorin as compared to 14C-phenylalanine showed a slower time course during both the middle and late stages of development, in keeping with the time needed for degradation of the protein. In accord with faster phe turnover near the end of adult development, total 14CO2 production was greater and the retention of 14C in hemolymph and fat body was less compared to the middle stage of development regardless of whether 14C-arylphorin or 14C-phe was injected. In the middle stage of development, the appearance of 14C in the cuticle and head parts was greater, whereas incorporation into abdomen and thorax was less than during the late stage of development. Since the pattern of 14C distribution from 14C-arylphorin and 14C-phe was similar, one major function of arylphorin must be as a storage protein replenishing the supply of free amino acids used for synthesis of adult tissues. These results also suggest a limited contribution of M. sexta arylphorin to formation of the cuticle subsequent to day-6 pharate adult.

  10. Surface plasmon resonance based fiber optic trichloroacetic acid sensor utilizing layer of silver nanoparticles and chitosan doped hydrogel

    Science.gov (United States)

    Semwal, Vivek; Shrivastav, Anand M.; Gupta, Banshi D.

    2017-02-01

    In this study, we report a silver nanoparticles/chitosan doped hydrogel-based fiber optic sensor for the detection of trichloroacetic acid (TCA). The sensor is based on the combined phenomenon of localized and propagating surface plasmons. The sensing relies on the interaction of TCA with silver nanoparticles (AgNP) which results in the electron transfer between the negative group of TCA and positive amino group of AgNP stabilizer (chitosan). This alters the mechanical properties/refractive index of the AgNP embedded hydrogel matrix as well as the refractive index of the AgNP. The change in refractive index of both in turn changes the effective refractive index of the nanocomposite hydrogel layer which can be determined using the Maxwell-Garnet Theory. Four stage optimization of the probe fabrication parameters is performed to obtain the best performance of the sensing probe. The sensor operates in the TCA concentration range 0-120 μm which is harmful for the humans and environment. The shift in peak extinction wavelength observed for the same TCA concentration range is 42 nm. The sensor has the linearity range for the TCA concentration range of 40-100 μm. The sensor possesses high sensitivity, selectivity and numerous other advantages such as ease of handling, quick response, modest cost and capability of online monitoring and remote sensing.

  11. Decontamination of a drinking water pipeline system contaminated with adenovirus and Escherichia coli utilizing peracetic acid and chlorine.

    Science.gov (United States)

    Kauppinen, Ari; Ikonen, Jenni; Pursiainen, Anna; Pitkänen, Tarja; Miettinen, Ilkka T

    2012-09-01

    A contaminated drinking water distribution network can be responsible for major outbreaks of infections. In this study, two chemical decontaminants, peracetic acid (PAA) and chlorine, were used to test how a laboratory-scale pipeline system can be cleaned after simultaneous contamination with human adenovirus 40 (AdV40) and Escherichia coli. In addition, the effect of the decontaminants on biofilms was followed as heterotrophic plate counts (HPC) and total cell counts (TCC). Real-time quantitative polymerase chain reaction (qPCR) was used to determine AdV40 and plate counting was used to enumerate E. coli. PAA and chlorine proved to be effective decontaminants since they decreased the levels of AdV40 and E. coli to below method detection limits in both water and biofilms. However, without decontamination, AdV40 remained present in the pipelines for up to 4 days. In contrast, the concentration of cultivable E. coli decreased rapidly in the control pipelines, implying that E. coli may be an inadequate indicator for the presence of viral pathogens. Biofilms responded to the decontaminants by decreased HPCs while TCC remained stable. This indicates that the mechanism of pipeline decontamination by chlorine and PAA is inactivation rather than physical removal of microbes.

  12. Flexible 75 kWel Stirling CHP-plant for bio-fuels with low emissions and a high fuel utilization. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    2011-07-01

    The objective of the project ''Flexible 75 kWel Stirling CHP-plant for bio-fuels with low emissions and a high fuel utilization'' was to combine the Danish experiences with the Stirling engine and updraft gasification with the application of the FLOX gas burner technology for developing and demonstrating a flexible biomass-based small scale CHP plant with 75 kW electrical output, high power efficiency and low emissions. Further, the project has aimed at increasing the technology's reliability and decreasing the need for service. Also, the project has included the development of a control and communication system for unmanned start-up and operation of the plant. During the project the objective was altered and so the development of a new Stirling engine design was done on the 4-cylindred 35 kWe Stirling engine instead of the 8-cylindred 75 kWe Stirling engine. Focus has been on designing a more durable engine designed for easy and fast service. Cold test of the engine has been successful and now full-scale hot tests are to be performed. In the project Stirling DK has also in cooperation with project partner Danish gas Technology Centre developed the Stirling Engine with Diluted Oxidation (SEDIOX) concept which is a combustion technology based on the diluted oxidation principle. A trademark is obtained and also a patent application is filed and pending regarding the SEDIOX combustion chamber concept. All components for the Stirling gasification plant were produced and installed at Svanholm Estate. The plant consisted of one conventional combustion chamber and one SD3E-type Stirling engine. The plant was commissioned in June 2009 and 1,472 hours of operation and 43 MWh of electricity production was achieved before the plant was de-commissioned in February 2010 due to divergences between Svanholm Estate and Stirling DK. During operation the control system including remote access was tested thoroughly and with great success. The new overall

  13. Association between CK-MB Area Under the Curve and Tranexamic Acid Utilization in Patients Undergoing Coronary Artery Bypass Surgery.

    Science.gov (United States)

    van Diepen, Sean; Merrill, Peter D; Carrier, Michel; Tardif, Jean-Claude; Podgoreanu, Mihai; Alexander, John H; Lopes, Renato D

    2017-05-01

    Myonecrosis after coronary artery bypass graft (CABG) surgery is associated with excess mortality. Tranexamic acid (TA), an anti-fibrinolytic agent, has been shown to reduce peri-operative blood loss without increasing the risk of myocardial infarction (MI); however, no large study has examined the association between TA treatment and post-CABG myonecrosis. In the MC-1 to Eliminate Necrosis and Damage in Coronary Artery Bypass Graft Surgery II trial, inverse probability weighting of the propensity to receive TA was used to test for differences among the 656 patients receiving and 770 patients not receiving TA. The primary outcome was creatine kinase MB (CK-MB) area under the curve (AUC) through 24 h. The secondary outcome was 30-day cardiovascular death or MI. Patients who received TA were more frequently female, had a previous MI, heart failure, low molecular weight heparin therapy, on-pump CABG, valvular surgery, and saphenous vein or radial grafts. The median 24-h CK-MB AUC was higher in TA-treated patients [301.9 (IQR 196.7-495.6) vs 253.5 (153.4-432.5) ng h/mL, p < 0.001]. No differences in the 30-day incidence of cardiovascular death or MI were observed (8.7 vs 8.3%, adjusted OR 0.99; 95% CI 0.67-1.45, p = 0.948). In patients undergoing CABG, TA use was associated with a higher risk of myonecrosis; however, no differences were observed in death or MI. Future larger studies should be directed at examining the pathophysiology of TA myonecrosis, and its association with subsequent clinical outcomes.

  14. Cold exposure enhances fat utilization but not non-esterified fatty acids, glycerol or catecholamines availability during submaximal walking and running

    Directory of Open Access Journals (Sweden)

    Dominique Daniel Gagnon

    2013-05-01

    Full Text Available Cold exposure modulates the use of carbohydrates and fat during exercise. This phenomenon has mostly been observed in controlled cycling studies, but not during walking and running when core temperature and oxygen consumption are controlled, as both may alter energy metabolism. This study aimed at examining energy substrate availability and utilization during walking and running in the cold when core temperature and oxygen consumption are maintained. Ten lightly clothed male subjects walked or ran for 60-min, at 50% and 70% of maximal oxygen consumption, respectively, in a climatic chamber set at 0°C or 22°C. Thermal, cardiovascular, and oxidative responses were measured every 15-min during exercise. Blood samples for serum non-esterified fatty acids, glycerol, glucose, beta-hydroxybutyrate, plasma catecholamines, and serum lipids were collected immediately prior, and at 30- and 60-min of exercise. Skin temperature strongly decreased while core temperature did not change during cold trials. Heart rate was also lower in cold trials. A rise in fat utilization in the cold was seen through lower respiratory quotient (-0.03 ± 0.02, greater fat oxidation (+0.14 ± 0.13 g•min-1 and contribution of fat to total energy expenditure (+1.62 ± 1.99 kcal•min-1. No differences from cold exposure were observed in blood parameters. During submaximal walking and running, a greater reliance on derived fat sources occurs in the cold, despite the absence of concurrent alterations in non-esterified fatty acids, glycerol, or catecholamine concentrations. This disparity may suggest a greater reliance on intra-muscular energy sources such as triglycerides during both walking and running.

  15. The Next Generation Non-competitive Active Polyester Nanosystems for Transferrin Receptor-mediated Peroral Transport Utilizing Gambogic Acid as a Ligand.

    Science.gov (United States)

    Saini, P; Ganugula, R; Arora, M; Kumar, M N V Ravi

    2016-01-01

    The current methods for targeted drug delivery utilize ligands that must out-compete endogenous ligands in order to bind to the active site facilitating the transport. To address this limitation, we present a non-competitive active transport strategy to overcome intestinal barriers in the form of tunable nanosystems (NS) for transferrin receptor (TfR) utilizing gambogic acid (GA), a xanthanoid, as its ligand. The NS made using GA conjugated poly(lactide-co-glycolide) (PLGA) have shown non-competitive affinity to TfR evaluated in cell/cell-free systems. The fluorescent PLGA-GA NS exhibited significant intestinal transport and altered distribution profile compared to PLGA NS in vivo. The PLGA-GA NS loaded with cyclosporine A (CsA), a model peptide, upon peroral dosing to rodents led to maximum plasma concentration of CsA at 6 h as opposed to 24 h with PLGA-NS with at least 2-fold higher levels in brain at 72 h. The proposed approach offers new prospects for peroral drug delivery and beyond.

  16. Analysis of the Clean Air Act Amendments of 1990: A forecast of the electric utility industry response to Title IV, Acid Deposition Control

    Energy Technology Data Exchange (ETDEWEB)

    Molburg, J.C.; Fox, J.A.; Pandola, G.; Cilek, C.M.

    1991-10-01

    The Clean Air Act Amendments of 1990 incorporate, for the first time, provisions aimed specifically at the control of acid rain. These provisions restrict emissions of sulfur dioxide (SO{sub 2}) and oxides of nitrogen (NO{sub x}) from electric power generating stations. The restrictions on SO{sub 2} take the form of an overall cap on the aggregate emissions from major generating plants, allowing substantial flexibility in the industry`s response to those restrictions. This report discusses one response scenario through the year 2030 that was examined through a simulation of the utility industry based on assumptions consistent with characterizations used in the National Energy Strategy reference case. It also makes projections of emissions that would result from the use of existing and new capacity and of the associated additional costs of meeting demand subject to the emission limitations imposed by the Clean Air Act. Fuel-use effects, including coal-market shifts, consistent with the response scenario are also described. These results, while dependent on specific assumptions for this scenario, provide insight into the general character of the likely utility industry response to Title IV.

  17. Analysis of the Clean Air Act Amendments of 1990: A forecast of the electric utility industry response to Title IV, Acid Deposition Control

    Energy Technology Data Exchange (ETDEWEB)

    Molburg, J.C.; Fox, J.A.; Pandola, G.; Cilek, C.M.

    1991-10-01

    The Clean Air Act Amendments of 1990 incorporate, for the first time, provisions aimed specifically at the control of acid rain. These provisions restrict emissions of sulfur dioxide (SO[sub 2]) and oxides of nitrogen (NO[sub x]) from electric power generating stations. The restrictions on SO[sub 2] take the form of an overall cap on the aggregate emissions from major generating plants, allowing substantial flexibility in the industry's response to those restrictions. This report discusses one response scenario through the year 2030 that was examined through a simulation of the utility industry based on assumptions consistent with characterizations used in the National Energy Strategy reference case. It also makes projections of emissions that would result from the use of existing and new capacity and of the associated additional costs of meeting demand subject to the emission limitations imposed by the Clean Air Act. Fuel-use effects, including coal-market shifts, consistent with the response scenario are also described. These results, while dependent on specific assumptions for this scenario, provide insight into the general character of the likely utility industry response to Title IV.

  18. Utilization of maize cob biochar and rice husk charcoal as soil amendments for improving acid soil fertility and productivity

    Directory of Open Access Journals (Sweden)

    Nurhidayati

    2014-10-01

    Full Text Available The decline in soil fertility in agricultural land is a major problem that causes a decrease in the production of food crops. One of the causes of the decline in soil fertility is declining soil pH that caused the decline in the availability of nutrients in the soil. This study aimed to assess the influence of alternative liming materials derived from maize cob biochar and rice husk charcoal compared to conventional lime to improve soil pH, soil nutrient availability and maize production. The experiment used a factorial complete randomized design which consisting of two factors. The first factor is the type of soil amendment which consists of three levels (calcite lime, rice husk charcoal and cob maize biochar. The second factor is the application rates of the soil amendment consisted of three levels (3, 6 and 9 t/ha and one control treatment (without soil amendment. The results of this study showed that the application of various soil amendment increased soil pH, which the pH increase of the lime application was relatively more stable over time compared to biochar and husk charcoal. The average of the soil pH increased for each soil amendment by 23% (lime, 20% (rice husk charcoal and 23% (biochar as compared with control. The increase in soil pH can increase the availability of soil N, P and K. The greatest influence of soil pH on nutrient availability was shown by the relationship between soil pH and K nutrient availability with R2 = 0.712, while for the N by R2 = 0.462 and for the P by R2 = 0.245. The relationship between the availability of N and maize yield showed a linear equation. While the relationship between the availability of P and K with the maize yield showed a quadratic equation. The highest maize yield was found in the application of biochar and rice husk charcoal with a dose of 6-9 t/ha. The results of this study suggested that biochar and husk charcoal could be used as an alternative liming material in improving acid soil

  19. Activation of β3-adrenoceptors increases in vivo free fatty acid uptake and utilization in brown but not white fat depots in high-fat-fed rats.

    Science.gov (United States)

    Warner, Amy; Kjellstedt, Ann; Carreras, Alba; Böttcher, Gerhard; Peng, Xiao-Rong; Seale, Patrick; Oakes, Nicholas; Lindén, Daniel

    2016-12-01

    Activation of brown adipose tissue (BAT) and browning of white adipose tissue (WAT) present potential new therapies for obesity and type 2 diabetes. Here, we examined the effects of β3-adrenergic stimulation on tissue-specific uptake and storage of free fatty acids (FFA) and its implications for whole body FFA metabolism in diet-induced obese rats using a multi-radiotracer technique. Male Wistar rats were high fat-fed for 12 wk and administered β3-agonist CL316,243 (CL, 1 mg·kg(-1)·day(-1)) or saline via osmotic minipumps during the last 3 wk. The rats were then fasted and acutely infused with a tracer mixture ([(14)C]palmitate and the partially metabolized R-[(3)H]bromopalmitate) under anesthesia. CL infusion decreased body weight gain and fasting plasma glucose levels. While core body temperature was unaffected, infrared thermography showed an increase in tail heat dissipation following CL infusion. Interestingly, CL markedly increased both FFA storage and utilization in interscapular and perirenal BAT, whereas the flux of FFA to skeletal muscle was decreased. In this rat model of obesity, only sporadic populations of beige adipocytes were detected in the epididymal WAT depot of CL-infused rats, and there was no change in FFA uptake or utilization in WAT following CL infusion. In summary, β3-agonism robustly increased FFA flux to BAT coupled with enhanced utilization. Increased BAT activation most likely drove the increased tail heat dissipation to maintain thermostasis. Our results emphasize the quantitative role of brown fat as the functional target of β3-agonism in obesity. Copyright © 2016 the American Physiological Society.

  20. Electrolytic regeneration of acid cupric chloride printed circuit board etchant. Final report, August 1, 1995--October 31, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Oxley, J.E.; Smialek, R.J.

    1997-04-18

    The overall objective of this ERIP program was to make substantial progress in further developing a process for electrolytic regeneration of acid cupric chloride etchant - a process which was initially demonstrated in in-house studies and EPA Phase I and Phase II SBIRs. Specific objectives of the work were: (1) to define optimum system operating conditions by conducting a systematic study of process parameters, (2) to develop or find a superior electrolyic cell separator material, (3) to determine an optimum activation procedure for the flow-through carbon/graphite felt electrodes which are so critical to process performance, (4) to demonstrate - on the pre-prototype scale - electrolytic compensation for oxygen ingress - which causes etchant solution growth, and (5) to begin engineering design work on a prototype-scale regeneration unit. Parametric studies looked at the effect that key plating parameters have on copper deposit quality. Parameters tested included (a) velocity past the plating cathodes, (b) copper concentration in the catholyte solution from which the copper is being plated, (c) plating current density, and (d) catholyte cupric ion concentration. The most significant effects were obtained for velocity changes. The work showed that catholyte velocities above 0.5 ft/sec were needed to get adequate plating at 77.5 mA/cm{sup 2} and higher currents, and that even higher flow was better.

  1. Study of the manufacturing costs of lead--acid batteries for peaking power. Final report for the period ending Oct. 1976

    Energy Technology Data Exchange (ETDEWEB)

    None

    1976-12-01

    A detailed study was made of a postulated 1000-MWh per year lead--acid battery business dedicated to supplying a single design of 40-MWh peaking power batteries to electric utilities. State-of-the-art industrial technology is assumed, but the manufacturing facility and business organization is tailored to the one product. Analysis of the product costs and business expenses associated with such an operation indicates that substantially lower selling prices can be realized as compared with normal industrial battery pricing. Under the low-risk conditions assumed, the selling price would be $36.90/kWh at the 4-h rate. Advanced technology would reduce the cost to $31.62/kWh. 21 figures, 31 tables. (RWR)

  2. Effect of ultrasonic irradiation on the number of acetylsalicylic acid crystals produced under the supersaturated condition and the ability of controlling the final crystal size via primary nucleation

    Science.gov (United States)

    Miyasaka, Etsuko; Kato, Yumi; Hagisawa, Minoru; Hirasawa, Izumi

    2006-03-01

    The purposes of this study were to investigate the effects of ultrasound irradiation on the number of crystals formed in an acetylsalicyclic acid crystallization process and to assess the controllability of the final product size via the number of primary nuclei. The number of crystals present after primary nucleation was counted and the relationship between the final product size and the number of crystals was examined. Additionally, the growing ASA crystals were observed, since ultrasound energy not only may control primary nucleation but may also the perfection of the crystal shape. At a high level of ultrasonic energy, ultrasound irradiation increased the average number of crystals, an effect that has been reported often; however, at a low level of ultrasonic energy it decreased the average number of crystals, and moreover, these opposing ultrasonic effects on the number of crystals interchanged at a specific energy threshold. These results reveal two novel phenomena—that there is an energy region where ultrasonic irradiation inhibits primary nucleation, and that a specific amount of ultrasonic energy is needed to activate primary nucleation. On the other hand, the final product size almost depended upon the number of primary nuclei, indicating that the final product size could be controlled via the number of crystals influenced by ultrasound irradiation. According to the photographs of crystals, they were not destroyed by the process. Therefore, it was proposed that ultrasound energy does not destroy the perfection of the crystal shape but only controls primary nucleation under the condition: both short irradiation time and low supersaturated condition.

  3. Utilization of subsurface microbial electrochemical systems to elucidate the mechanisms of competition between methanogenesis and microbial iron(III)/humic acid reduction in Arctic peat soils

    Science.gov (United States)

    Friedman, E. S.; Miller, K.; Lipson, D.; Angenent, L. T.

    2012-12-01

    High-latitude peat soils are a major carbon reservoir, and there is growing concern that previously dormant carbon from this reservoir could be released to the atmosphere as a result of continued climate change. Microbial processes, such as methanogenesis and carbon dioxide production via iron(III) or humic acid reduction, are at the heart of the carbon cycle in Arctic peat soils [1]. A deeper understanding of the factors governing microbial dominance in these soils is crucial for predicting the effects of continued climate change. In previous years, we have demonstrated the viability of a potentiostatically-controlled subsurface microbial electrochemical system-based biosensor that measures microbial respiration via exocellular electron transfer [2]. This system utilizes a graphite working electrode poised at 0.1 V NHE to mimic ferric iron and humic acid compounds. Microbes that would normally utilize these compounds as electron acceptors donate electrons to the electrode instead. The resulting current is a measure of microbial respiration with the electrode and is recorded with respect to time. Here, we examine the mechanistic relationship between methanogenesis and iron(III)- or humic acid-reduction by using these same microbial-three electrode systems to provide an inexhaustible source of alternate electron acceptor to microbes in these soils. Chamber-based carbon dioxide and methane fluxes were measured from soil collars with and without microbial three-electrode systems over a period of four weeks. In addition, in some collars we simulated increased fermentation by applying acetate treatments to understand possible effects of continued climate change on microbial processes in these carbon-rich soils. The results from this work aim to increase our fundamental understanding of competition between electron acceptors, and will provide valuable data for climate modeling scenarios. 1. Lipson, D.A., et al., Reduction of iron (III) and humic substances plays a major

  4. Novel Approaches to Immobilized Heteropoly Acid Systems for High Temperature, Low Relative Humidity Polymer-Type Membranes - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Herring, Andrew M; Horan, James L; Aieta, Niccolo V; Sachdeva, Sonny; Kuo, Mei-Chen; Ren, Hui; Lingutla, Anitha; Emery, Michael; Haugen, Gregory M; Yandrasits, Michael A; Sharma, Neeraj; Coggio, William D; Hamrock, Steven J; Frey, Matthew H

    2012-05-20

    Original research was carried out at the CSM and the 3M Company from March 2007 through September 2011. The research was aimed at developing new to the world proton electrolyte materials for use in hydrogen fuel cells, in particular with high proton conductivity under hot and dry conditions (>100mS/cm at 120°C and 50%RH). Broadly stated, the research at 3M and between 3M and CSM that led to new materials took place in two phases: In the first phase, hydrocarbon membranes that could be formed by photopolymerization of monomer mixtures were developed for the purpose of determining the technical feasibility of achieving the program's Go/No-Go decision conductivity target of >100mS/cm at 120°C and 50%RH. In the second phase, attempts were made to extend the achieved conductivity level to fluorinated material systems with the expectation that durability and stability would be improved (over the hydrocarbon material). Highlights included: Multiple lots of an HPA-immobilized photocurable terpolymer derived from di-vinyl-silicotungstic acid (85%), n-butyl acrylate, and hexanediol diacrylate were prepared at 3M and characterized at 3M to exhibit an initial conductivity of 107mS/cm at 120°C and 47%RH (PolyPOM85v) using a Bekktech LLC sample fixture and TestEquity oven. Later independent testing by Bekktech LLC, using a different preheating protocol, on the same material, yielded a conductivity value of approximately 20mS/cm at 120°C and 50%RH. The difference in measured values is likely to have been the result of an instability of properties for the material or a difference in the measurement method. A dispersed catalyst fuel cell was fabricated and tested using a 150¼m thick HPA-based photocurable membrane (above, PolyPOM75v), exhibiting a current density of greater than 300mA/cm2 at 0.5V (H2/Air 800/1800sccm 70°C/75%RH ambient outlet pressure). Multiple lots of a co-polymer based on poly-trifluorovinylether (TFVE) derived HPA were synthesized and fabricated into

  5. Pareto utility

    NARCIS (Netherlands)

    Masako, I.; Laeven, R.J.A.; Magnus, J.R.; Muris, C.

    2013-01-01

    In searching for an appropriate utility function in the expected utility framework, we formulate four properties that we want the utility function to satisfy. We conduct a search for such a function, and we identify Pareto utility as a function satisfying all four desired properties. Pareto utility

  6. Paralegal Studies Program. Curriculum Utilization. Final Report.

    Science.gov (United States)

    Humphrey, Doris D.

    A program developed a paralegal studies program for Delaware County Community College in the Philadelphia (Pennsylvania) metropolitan area. Activities included gathering of information on paralegal studies as it related to curriculum content; advisory group establishment; curriculum development; training/educational material development; and…

  7. Utilization of FEP energetics. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Frederking, T.H.K.; Abbassi, P.; Afifi, F.; Khandhar, P.K.; Ono, D.Y.; Chen, W.E.W.

    1987-12-01

    The research and development work on Fountain Effect Pump Systems (FEP systems) has been of interest in the competition between mechanical pumps for He II and FEP units. The latter do not have moving parts. In the course of the work, the energetics have been addressed using one part of a simple four-changes-of-state cycle. One option is the FEP ideal change of state at constant chemical potential (mu). The other option is the two-state sequence mu-P with a d mu=0 state change followed by an isobar. Questions of pump behavior, of flow rate response to temperature difference at the hot end, and related questions of thermodynamic cycle completion and heat transfer have been addressed. Porous media data obtained elucidate differences between vapor-liquid phase separation (VLPS) and Zero Net Mass Transfer (ZNMF).

  8. Final Environmental Planning Technical Report. Utilities

    Science.gov (United States)

    1984-01-01

    Manpower Requirements by Year 1-8 1.1-2 Total Jobs, Local and Regional Hires, and Inmigration 1-8 for the Employment Demand Region of Influence 1.1-3...weekly commuters and inmigrants , on an annual average basis. In general, locally available labor will fill all the road and construction jobs. 1-6 Lf...employment. Table 1.1-2 TOTAL JOBS, LOCAL AND REGIONAL HIRES, AND INMIGRATION FOR THE EMPLOYMENT DEMAND REGION OF INFLUENCE 1991 and 1984 1985 1986 1987

  9. Desalination utilizing clathrate hydrates (LDRD final report).

    Energy Technology Data Exchange (ETDEWEB)

    Simmons, Blake Alexander; Bradshaw, Robert W.; Dedrick, Daniel E.; Cygan, Randall Timothy (Sandia National Laboratories, Albuquerque, NM); Greathouse, Jeffery A. (Sandia National Laboratories, Albuquerque, NM); Majzoub, Eric H. (University of Missouri, Columbia, MO)

    2008-01-01

    Advances are reported in several aspects of clathrate hydrate desalination fundamentals necessary to develop an economical means to produce municipal quantities of potable water from seawater or brackish feedstock. These aspects include the following, (1) advances in defining the most promising systems design based on new types of hydrate guest molecules, (2) selection of optimal multi-phase reactors and separation arrangements, and, (3) applicability of an inert heat exchange fluid to moderate hydrate growth, control the morphology of the solid hydrate material formed, and facilitate separation of hydrate solids from concentrated brine. The rate of R141b hydrate formation was determined and found to depend only on the degree of supercooling. The rate of R141b hydrate formation in the presence of a heat exchange fluid depended on the degree of supercooling according to the same rate equation as pure R141b with secondary dependence on salinity. Experiments demonstrated that a perfluorocarbon heat exchange fluid assisted separation of R141b hydrates from brine. Preliminary experiments using the guest species, difluoromethane, showed that hydrate formation rates were substantial at temperatures up to at least 12 C and demonstrated partial separation of water from brine. We present a detailed molecular picture of the structure and dynamics of R141b guest molecules within water cages, obtained from ab initio calculations, molecular dynamics simulations, and Raman spectroscopy. Density functional theory calculations were used to provide an energetic and molecular orbital description of R141b stability in both large and small cages in a structure II hydrate. Additionally, the hydrate of an isomer, 1,2-dichloro-1-fluoroethane, does not form at ambient conditions because of extensive overlap of electron density between guest and host. Classical molecular dynamics simulations and laboratory trials support the results for the isomer hydrate. Molecular dynamics simulations show that R141b hydrate is stable at temperatures up to 265K, while the isomer hydrate is only stable up to 150K. Despite hydrogen bonding between guest and host, R141b molecules rotated freely within the water cage. The Raman spectrum of R141b in both the pure and hydrate phases was also compared with vibrational analysis from both computational methods. In particular, the frequency of the C-Cl stretch mode (585 cm{sup -1}) undergoes a shift to higher frequency in the hydrate phase. Raman spectra also indicate that this peak undergoes splitting and intensity variation as the temperature is decreased from 4 C to -4 C.

  10. Energy utilization: municipal waste incineration. Final report

    Energy Technology Data Exchange (ETDEWEB)

    LaBeck, M.F.

    1981-03-27

    An assessment is made of the technical and economical feasibility of converting municipal waste into useful and useable energy. The concept presented involves retrofitting an existing municipal incinerator with the systems and equipment necessary to produce process steam and electric power. The concept is economically attractive since the cost of necessary waste heat recovery equipment is usually a comparatively small percentage of the cost of the original incinerator installation. Technical data obtained from presently operating incinerators designed specifically for generating energy, documents the technical feasibility and stipulates certain design constraints. The investigation includes a cost summary; description of process and facilities; conceptual design; economic analysis; derivation of costs; itemized estimated costs; design and construction schedule; and some drawings.

  11. Structural evolution of trimesic acid (TMA)/Zn2 + ion network on Au(111) to final structure of (10√3 × 10√3)

    Science.gov (United States)

    Kim, Jandee; Lee, Jaesung; Rhee, Choong Kyun

    2016-02-01

    Presented is a scanning tunneling microscopy (STM) study of structural evolution of TMA/Zn2 + ion network on Au(111) to the final structure of (10√3 × 10√3) during solution phase post-modification of pristine trimesic acid (TMA) network of a (5√3 × 5√3) structure with Zn2 + ions. Coordination of Zn2 + ions into adsorbed TMA molecules transforms crown-like TMA hexamers in pristine TMA network to chevron pairs in TMA/Zn2 + ion network. Two ordered transient structures of TMA/Zn2 + ion network were observed. One is a (5√7 × 5√7) structure consisting of Zn2 + ion-containing chevron pairs and Zn2 + ion-free TMA dimers. The other is a (5√39 × 5√21) structure made of chevron pairs and chevron-pair-missing sites. An STM image showing domains of different stages of crystallization of chevron pairs demonstrates that the TMA/Zn2 + network before reaching to the final one is quite dynamic. The observed structural evolution of the TMA/Zn2 + ion network is discussed in terms of modification of configurations of adsorbed TMA as accommodating Zn2 + ions and re-ordering of Zn2 + ion-containing chevron pairs.

  12. The purple acid phosphatase GmPAP21 enhances internal phosphorus utilization and possibly plays a role in symbiosis with rhizobia in soybean.

    Science.gov (United States)

    Li, Chengchen; Li, Caifeng; Zhang, Haiyan; Liao, Hong; Wang, Xiurong

    2017-02-01

    Induction of secreted and intracellular purple acid phosphatases (PAPs; EC 3.1.3.2) is widely recognized as an adaptation of plants to phosphorus (P) deficiency. The secretion of PAPs plays important roles in P acquisition. However, little is known about the functions of intracellular PAP in plants and nodules. In this study, we identified a novel PAP gene GmPAP21 in soybean. Expression of GmPAP21 was induced by P limitation in nodules, roots and old leaves, and increased in roots with increasing duration of P starvation. Furthermore, the induction of GmPAP21 in nodules and roots was more intensive than in leaves in both P-efficient genotype HN89 and P-inefficient genotype HN112 in response to P starvation, and the relative expression in the leaves and nodules of HN89 was significantly greater than that of HN112 after P deficiency treatment. Further functional analyses showed that over-expressing GmPAP21 significantly enhanced both acid phosphatase activity and growth performance of hairy roots under P starvation condition, indicating that GmPAP21 plays an important role in P utilization. Moreover, GUS expression driven by GmPAP21 promoter was shown in the nodules besides roots. Overexpression of GmPAP21 in transgenic soybean significantly inhibited nodule growth, and thereby affected plant growth after inoculation with rhizobia. This suggests that GmPAP21 is also possibly involved in regulating P metabolism in nodules. Taken together, our results suggest that GmPAP21 is a novel plant PAP that functions in the adaptation of soybean to P starvation, possibly through its involvement in P recycling in plants and P metabolism in nodules.

  13. Identification of leads through in silico approaches utilizing benzylthio-1H-benzo[d]imidazol-1-yl acetic acid derivatives: A potent CRTh2 antagonist

    Science.gov (United States)

    Babu, Sathya; Kulkarni, Seema A.; Sohn, Honglae; Madhavan, Thirumurthy

    2015-12-01

    Chemoattractant Receptor-homologous molecule expressed on Th2 cells (CRTh2) is considered as a potential therapeutic target for the treatment of asthma and allergic rhinitis. Herein, we describe the pharmacophore based virtual screening combined with molecular docking and 3D-QSAR methods to identify new potent CRTh2 inhibitors. Several pharmacophore models were generated and validated by Guner-Henry scoring method. The best models were utilized as 3D Pharmacophore query to screen against ZINC database and the retrieved hits were further validated by fitness score, Lipinski's rule of five, Surflex docking and Comparative Molecular Field Analysis (CoMFA) process. The optimum CoMFA model was developed using known inhibitors and the predictive ability of model was examined by statistical parameters like q2 = 0.552 and r2pred = 0.636. The biological activities of the screened compounds were calculated using the generated CoMFA model. Finally nine compounds were found to have good potential and high inhibitory activities and they may act as novel lead compounds for CRTh2 inhibitor designing.

  14. A comparison of sole carbon source utilization patterns and phospholipid fatty acid profiles to detect changes in the root microflora of hydroponically grown crops.

    Science.gov (United States)

    Khalil, S; Bååth, E; Alsanius, B; Englund, J E; Sundin, P; Gertsson, U E; Jensén, P

    2001-04-01

    Sole carbon source utilization (SCSU) patterns and phospholipid fatty acid (PLFA) profiles were compared with respect to their potential to characterize root-inhabiting microbial communities of hydroponically grown crops. Sweet pepper (Capsicum annum cv. Evident), lettuce (Lactuca sativa cv. Grand Rapids), and four different cultivars of tomato (Lycopersicon esculentum cvs. Gitana, Armada, Aromata, and Elin) were grown in 1-L black plastic beakers placed in a cultivation chamber with artificial light. In addition to the harvest of the plants after 6 weeks, plants of one tomato cultivar, cv. Gitana, were also harvested after 4 and 8 weeks. The cultivation in this study was performed twice. Principal component analysis was used to analyze the data. Both characterization methods had the ability to discriminate between the root microflora of different plant species, cultivars, and one tomato cultivar at different ages. Differences in both SCSU patterns and PLFA profiles were larger between plant species than between cultivars, but for both methods the largest differences were between the two cultivations. Still, the differences between treatments were always due to differences in the same PLFAs in both cultivations. This was not the case for the SCSU patterns when different plant ages were studied. Furthermore, PLFA profiles showed less variation between replicates than did SCSU patterns. This larger variation observed among the SCSU data indicates that PLFA may be more useful to detect changes in the root microflora of hydroponically grown crops than the SCSU technique.

  15. Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization

    Directory of Open Access Journals (Sweden)

    Chengqiang Wang

    2015-09-01

    Full Text Available The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae [1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisiae. The fluorescence of fusion protein Mgt05196p-GFP expressing strain was located on the cell surface demonstrated that the heterogenous transporter Mgt05196p was targeted to the plasma membrane of S. cerevisiae. The expressing of Mgt05196p in the hxt null S. cerevisiae endowed the strain with the glucose and d-xylose absorption capacity, as well as expressing the native d-xylose transporter Gal2p. The transmembrane domains of Mgt05196p were predicted and compared with the XylEp, whose crystal structure was revealed. And then, the homologous modeling of Mgt05196p was built basing on the XylEp to find out the crucial amino acid residues for sugars binding and transport.

  16. A study of toxic emissions from a coal-fired power plant utilizing the SNOX innovative clean coal technology demonstration. Volume 1, Sampling/results/special topics: Final report

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-01

    This study was one of a group of assessments of toxic emissions from coal-fired power plants, conducted for DOE during 1993. The motivation for those assessments was the mandate in the 1990 Clean Air Act Amendments that a study be made of emissions of hazardous air pollutants (HAPs) from electric utilities. The report is organized in two volumes. Volume 1: Sampling describes the sampling effort conducted as the basis for this study; Results presents the concentration data on HAPs in the several power plant streams, and reports the results of evaluations and calculations conducted with those data; and Special Topics report on issues such as comparison of sampling methods and vapor/solid distributions of HAPs. Volume 2: Appendices include quality assurance/quality control results, uncertainty analysis for emission factors, and data sheets. This study involved measurements of a variety of substances in solid, liquid, and gaseous samples from input, output, and process streams at the Innovative Clean Coal Technology Demonstration (ICCT) of the Wet Sulfuric Acid-Selective Catalytic Reduction (SNOX) process. The SNOX demonstration is being conducted at Ohio Edison`s Niles Boiler No. 2 which uses cyclone burners to burn bituminous coal. A 35 megawatt slipstream of flue gas from the boiler is used to demonstrate SNOX. The substances measured at the SNOX process were the following: 1. Five major and 16 trace elements, including mercury, chromium, cadmium, lead, selenium, arsenic, beryllium, and nickel; 2. Acids and corresponding anions (HCl, HF, chloride, fluoride, phosphate, sulfate); 3. Ammonia and cyanide; 4. Elemental carbon; 5. Radionuclides; 6. Volatile organic compounds (VOC); 7. Semi-volatile compounds (SVOC) including polynuclear aromatic hydrocarbons (PAH); and 8. Aldehydes.

  17. YEAR 2 BIOMASS UTILIZATION

    Energy Technology Data Exchange (ETDEWEB)

    Christopher J. Zygarlicke

    2004-11-01

    This Energy & Environmental Research Center (EERC) Year 2 Biomass Utilization Final Technical Report summarizes multiple projects in biopower or bioenergy, transportation biofuels, and bioproducts. A prototype of a novel advanced power system, termed the high-temperature air furnace (HITAF), was tested for performance while converting biomass and coal blends to energy. Three biomass fuels--wood residue or hog fuel, corn stover, and switchgrass--and Wyoming subbituminous coal were acquired for combustion tests in the 3-million-Btu/hr system. Blend levels were 20% biomass--80% coal on a heat basis. Hog fuel was prepared for the upcoming combustion test by air-drying and processing through a hammer mill and screen. A K-Tron biomass feeder capable of operating in both gravimetric and volumetric modes was selected as the HITAF feed system. Two oxide dispersion-strengthened (ODS) alloys that would be used in the HITAF high-temperature heat exchanger were tested for slag corrosion rates. An alumina layer formed on one particular alloy, which was more corrosion-resistant than a chromia layer that formed on the other alloy. Research activities were completed in the development of an atmospheric pressure, fluidized-bed pyrolysis-type system called the controlled spontaneous reactor (CSR), which is used to process and condition biomass. Tree trimmings were physically and chemically altered by the CSR process, resulting in a fuel that was very suitable for feeding into a coal combustion or gasification system with little or no feed system modifications required. Experimental procedures were successful for producing hydrogen from biomass using the bacteria Thermotoga, a deep-ocean thermal vent organism. Analytical procedures for hydrogen were evaluated, a gas chromatography (GC) method was derived for measuring hydrogen yields, and adaptation culturing and protocols for mutagenesis were initiated to better develop strains that can use biomass cellulose. Fly ash derived from

  18. YEAR 2 BIOMASS UTILIZATION

    Energy Technology Data Exchange (ETDEWEB)

    Christopher J. Zygarlicke

    2004-11-01

    This Energy & Environmental Research Center (EERC) Year 2 Biomass Utilization Final Technical Report summarizes multiple projects in biopower or bioenergy, transportation biofuels, and bioproducts. A prototype of a novel advanced power system, termed the high-temperature air furnace (HITAF), was tested for performance while converting biomass and coal blends to energy. Three biomass fuels--wood residue or hog fuel, corn stover, and switchgrass--and Wyoming subbituminous coal were acquired for combustion tests in the 3-million-Btu/hr system. Blend levels were 20% biomass--80% coal on a heat basis. Hog fuel was prepared for the upcoming combustion test by air-drying and processing through a hammer mill and screen. A K-Tron biomass feeder capable of operating in both gravimetric and volumetric modes was selected as the HITAF feed system. Two oxide dispersion-strengthened (ODS) alloys that would be used in the HITAF high-temperature heat exchanger were tested for slag corrosion rates. An alumina layer formed on one particular alloy, which was more corrosion-resistant than a chromia layer that formed on the other alloy. Research activities were completed in the development of an atmospheric pressure, fluidized-bed pyrolysis-type system called the controlled spontaneous reactor (CSR), which is used to process and condition biomass. Tree trimmings were physically and chemically altered by the CSR process, resulting in a fuel that was very suitable for feeding into a coal combustion or gasification system with little or no feed system modifications required. Experimental procedures were successful for producing hydrogen from biomass using the bacteria Thermotoga, a deep-ocean thermal vent organism. Analytical procedures for hydrogen were evaluated, a gas chromatography (GC) method was derived for measuring hydrogen yields, and adaptation culturing and protocols for mutagenesis were initiated to better develop strains that can use biomass cellulose. Fly ash derived from

  19. Effect of conjugated linoleic acid supplementation on body composition, body fat mobilization, protein accretion, and energy utilization in early lactation dairy cows.

    Science.gov (United States)

    von Soosten, D; Meyer, U; Piechotta, M; Flachowsky, G; Dänicke, S

    2012-03-01

    The objective of the study was to investigate the effects of trans-10,cis-12 and cis-9,trans-11 conjugated linoleic acid (CLA) supplementation on body composition, mobilization or accretion of body fat and protein mass, as well as the energy metabolism of dairy cows during the first 105 d in milk (DIM). For this purpose, a comparative slaughter experiment was conducted with 25 primiparous German Holstein cows. The experiment started at 1 DIM with the slaughter of 5 animals of an initial group receiving no CLA supplement. The remaining animals were fed a CLA supplement (n=10) or a stearic acid-based control fat supplement (CON; n=10) from 1 DIM up to slaughter. After 42 DIM, 5 more cows from each treatment (42-CLA and 42-CON) were slaughtered. The remaining 5 cows in each treatment were slaughtered after 105 DIM (105-CLA and 105-CON). The animals of the CLA groups consumed 6.0 g/d of trans-10,cis-12 CLA and 5.7 g/d of cis-9,trans-11 CLA. During the slaughter process, the empty body mass was recorded and partitioned into 9 fractions (meat, bone, offal, hide, mammary gland, retroperitoneal fat, omental fat, mesenteric fat, and s.c. fat). The fractions were analyzed for dry matter, ether extract, crude protein, and ash to calculate the body composition of the empty body mass at the different slaughter times. The principle of the comparative slaughter technique was applied to estimate body fat or protein mobilization and accretion in the viewed periods from 1 DIM until 42 and 105 DIM. The heat production (HP) was calculated by subtracting the energy in milk and energy changes in body mass from the metabolizable energy intake. The body composition was not affected by CLA supplementation. However, the mobilization of body fat mass from 1 until 42 DIM was 24.1 kg in the 42-CON group and 14.3 kg in the 42-CLA group. This resulted in a trend to lower body mass (fat and protein) mobilization of 10.5 kg in the 42-CLA group. Energy mobilization from body mass was 21.2 MJ/d in

  20. Burr Utility

    NARCIS (Netherlands)

    Ikefuji, M.; Laeven, R.J.A.; Magnus, J.R.; Muris, C.H.M.

    2010-01-01

    This note proposes the Burr utility function. Burr utility is a flexible two-parameter family that behaves approximately power-like (CRRA) remote from the origin, while exhibiting exponential-like (CARA) features near the origin. It thus avoids the extreme behavior of the power family near the origi

  1. Estimating Utility

    DEFF Research Database (Denmark)

    Arndt, Channing; Simler, Kenneth R.

    2010-01-01

    an information-theoretic approach to estimating cost-of-basic-needs (CBN) poverty lines that are utility consistent. Applications to date illustrate that utility-consistent poverty measurements derived from the proposed approach and those derived from current CBN best practices often differ substantially......, with the current approach tending to systematically overestimate (underestimate) poverty in urban (rural) zones....

  2. Cultured Representatives of Two Major Phylogroups of Human Colonic Faecalibacterium prausnitzii Can Utilize Pectin, Uronic Acids, and Host-Derived Substrates for Growth

    NARCIS (Netherlands)

    Lopez-Siles, Mireia; Khan, Tanweer M.; Duncan, Sylvia H.; Harmsen, Hermie J. M.; Garcia-Gil, L. Jesus; Flint, Harry J.

    Faecalibacterium prausnitzii is one of the most abundant commensal bacteria in the healthy human large intestine, but information on genetic diversity and substrate utilization is limited. Here, we examine the phylogeny, phenotypic characteristics, and influence of gut environmental factors on

  3. Digestion, absorption and utilization of single-cell protein by the preruminant calf. The true digestibility of milk and bacterial protein and the apparent digestibility and utilization of their constituent amino acids.

    Science.gov (United States)

    Sedgman, C A; Roy, J H; Thomas, J; Stobo, I J; Ganderton, P

    1985-07-01

    Two experiments of Latin square design were made, each with four Friesian bull calves fitted with re-entrant duodenal and ileal cannulas at 4-10 d of age. The calves were used to study the effect of giving milk-substitutes containing 0, 300, 500 and 700 g bacterial protein (Pruteen)/kg total protein on apparent digestibility of nitrogen fractions and amino acids and true digestibility of 3H-labelled milk protein and 35S-labelled bacterial protein in the small intestine. A third experiment of Latin square design with four intact Friesian calves was used to measure apparent digestibility of nutrients throughout the alimentary tract and retention of N, calcium and phosphorus. At the duodenum, volume of outflow, its pH, and outflow of total-N(TN), protein-N (PN) and non-protein-N (NPN) decreased with time after feeding. At the ileum, volume of outflow and TN outflow were unaffected by time after feeding but PN outflow decreased; NPN outflow at the ileum increased to a maximum 6 h after feeding and then declined. Increased inclusion of Pruteen did not affect the volume of outflow at the duodenum or ileum, but duodenal PN outflow increased. At the ileum, pH values were lower and TN, PN and NPN outflows were higher with increasing concentration of Pruteen in the diet. Apparent digestibility in the small intestine tended to decrease with greater amounts of Pruteen, but was only significant for NPN. Apparent digestibility from mouth to ileum significantly decreased for TN and PN as Pruteen inclusion increased. Amino acid concentration in duodenal outflow, with the exception of that of arginine, reflected intake. The total amount of each amino acid in ileal outflow increased and the apparent digestibility of most amino acids decreased with greater amounts of Pruteen in the diet. Apparent digestibility of nucleic acid-N from Pruteen was very high. True digestibility in the small intestine and between mouth and ileum of 3H-labelled milk protein was high and did not differ

  4. In situ detoxification of dry dilute acid pretreated corn stover by co-culture of xylose-utilizing and inhibitor-tolerant Saccharomyces cerevisiae increases ethanol production.

    Science.gov (United States)

    Zhu, Jia-Qing; Li, Xia; Qin, Lei; Li, Wen-Chao; Li, Hui-Ze; Li, Bing-Zhi; Yuan, Ying-Jin

    2016-10-01

    Co-culture of xylose-utilizing and inhibitor-tolerant Saccharomyces cerevisiae was developed for bioethanol production from undetoxified pretreated biomass in simultaneously saccharification and co-fermentation (SSCF) process. Glucose accumulation during late fermentation phase in SSCF using xylose-utilizing strain can be eliminated by the introduction of inhibitor-tolerant strain. Effect of different ratios of two strains was investigated and xylose-utilizing strain to inhibitor-tolerant strain ratio of 10:1 (w/w) showed the best xylose consumption and the highest ethanol yield. Inoculating of xylose-utilizing strain at the later stage of SSCF (24-48h) exhibited lower ethanol yield than inoculating at early stage (the beginning 0-12h), probably due to the reduced enzymatic efficiency caused by the unconsumed xylose and oligomeric sugars. Co-culture SSCF increased ethanol concentration by 21.2% and 41.0% comparing to SSCF using individual inhibitor-tolerant and xylose-utilizing strain (increased from 48.5 and 41.7g/L to 58.8g/L), respectively, which suggest this co-culture system was very promising.

  5. Utilizing maleic acid as a novel fuel for synthesis of PbFe{sub 12}O{sub 19} nanoceramics via sol–gel auto-combustion route

    Energy Technology Data Exchange (ETDEWEB)

    Ansari, Fatemeh; Soofivand, Faezeh; Salavati-Niasari, Masoud, E-mail: salavati@kashanu.ac.ir

    2015-05-15

    PbFe{sub 12}O{sub 19} nanostructures were prepared in an aqueous solution by the sol–gel auto-combustion method using Pb(NO{sub 3}){sub 2} and Fe(NO{sub 3}){sub 3} as starting materials and various carboxylic acids, including oxalic acid, malonic acid, succinic acid and maleic acid as fuel and reducing and capping agents. The as-synthesized products were characterized by X- ray diffraction, scanning electron microscopy, and X-ray energy dispersive spectroscopy. The effect of carboxylic acid type, Pb{sup +} {sup 2} to carboxylic acid molar ratio, and calcination temperature was investigated on the morphology of the products and several experiments were carried out to obtain the optimal reaction conditions. It was found that the phase and the morphology of the products are influenced by the investigated parameters. Furthermore, vibrating sample magnetometer (VSM) was used to study the magnetic properties of PbFe{sub 12}O{sub 19} samples. - Graphical abstract: Display Omitted - Highlights: • PbFe{sub 12}O{sub 19} nanoceramics were synthesized from Fe(NO{sub 3}){sub 3} and Pb(NO{sub 3}){sub 2} via the sol–gel auto combustion method. • The maleic acid can be instead of common capping agent and fuel in auto-combustion sol–gel. • The synthesized PbFe{sub 12}O{sub 19} is a hard magnetic material. • The specific saturation magnetization and coercivity are 27 emu/g and 1900 Oe, respectively.

  6. Utilization of High-Fructose Corn Syrup for Biomass Production Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Aurantiochytrium sp. YLH70.

    Science.gov (United States)

    Yu, Xin-Jun; Yu, Zhi-Qiang; Liu, Ying-Liang; Sun, Jie; Zheng, Jian-Yong; Wang, Zhao

    2015-11-01

    High-fructose corn syrup (HFCS) is an agro-source product and has been the most commonly used substitute for sugar as sweetener in food industry due to its low price and high solution property. In this study, the F55 HFCS, rich in fructose and glucose, was first tested for biomass and docosahexaenoic acid productions as a mixed carbon source by a newly isolated Aurantiochytrium sp.YLH70. After the compositions of the HFCS media were optimized, the results showed that the HFCS with additions of metal ion and vitamin at low concentrations was suitable for biomass and docosahexaenoic acid productions and the metal ion and sea salt had the most significant effects on biomass production. During the 5-l fed-batch fermentation, total HFCS containing 180 g l(-1) reducing sugar was consumed and yields of biomass, lipid, and DHA could reach 78.5, 51, and 20.1 g l(-1), respectively, at 114 h. Meanwhile, the daily productivity and the reducing sugar conversion yield for docosahexaenoic acid were up to 4.23 g l(-1)day(-1) and 0.11 g g(-1). The fatty acid profile of Aurantiochytrium sp.YLH70 showed that 46.4% of total fatty acid was docosahexaenoic acid, suggesting that Aurantiochytrium sp.YLH70 was a promising DHA producer.

  7. PPARd activation in human myotubes increases mitochondrial fatty acid oxidative capacity and reduces glucose utilization by a switch in substrate preference

    NARCIS (Netherlands)

    Feng, Y.; Nikolic, N.; Bakke, S.S.; Kersten, A.H.; Boekschoten, M.V.

    2014-01-01

    The role of peroxisome proliferator-activated receptor d (PPARd) activation on global gene expression and mitochondrial fuel utilization were investigated in human myotubes. Only 21 genes were up-regulated and 3 genes were down-regulated after activation by the PPARd agonist GW501516. Pathway analys

  8. Fermentative utilization of coffee mucilage using Bacillus coagulans and investigation of down-stream processing of fermentation broth for optically pure l(+)-lactic acid production.

    Science.gov (United States)

    Neu, Anna-Katrin; Pleissner, Daniel; Mehlmann, Kerstin; Schneider, Roland; Puerta-Quintero, Gloria Inés; Venus, Joachim

    2016-07-01

    In this study, mucilage, a residue from coffee production, was investigated as substrate in fermentative l(+)-lactic acid production. Mucilage was provided as liquid suspension consisting glucose, galactose, fructose, xylose and sucrose as free sugars (up to 60gL(-1)), and used directly as medium in Bacillus coagulans batch fermentations carried out at 2 and 50L scales. Using mucilage and 5gL(-1) yeast extract as additional nitrogen source, more than 40gL(-1) lactic acid was obtained. Productivity and yield were 4-5gL(-1)h(-1) and 0.70-0.77g lactic acid per g of free sugars, respectively, irrespective the scale. Similar yield was found when no yeast extract was supplied, the productivity, however, was 1.5gL(-1)h(-1). Down-stream processing of culture broth, including filtration, electrodialysis, ion exchange chromatography and distillation, resulted in a pure lactic acid formulation containing 930gL(-1)l(+)-lactic acid. Optical purity was 99.8%. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Final report of the safety assessment of L-Ascorbic Acid, Calcium Ascorbate, Magnesium Ascorbate, Magnesium Ascorbyl Phosphate, Sodium Ascorbate, and Sodium Ascorbyl Phosphate as used in cosmetics.

    Science.gov (United States)

    Elmore, Amy R

    2005-01-01

    L-Ascorbic Acid, Calcium Ascorbate, Magnesium Ascorbate, Magnesium Ascorbyl Phosphate, Sodium Ascorbate, and Sodium Ascorbyl Phosphate function in cosmetic formulations primarily as antioxidants. Ascorbic Acid is commonly called Vitamin C. Ascorbic Acid is used as an antioxidant and pH adjuster in a large variety of cosmetic formulations, over 3/4 of which were hair dyes and colors at concentrations between 0.3% and 0.6%. For other uses, the reported concentrations were either very low (Sodium Ascorbyl Phosphate functions as an antioxidant in cosmetic products and is used at concentrations ranging from 0.01% to 3%. Magnesium Ascorbyl Phosphate functions as an antioxidant in cosmetics and was reported being used at concentrations from 0.001% to 3%. Sodium Ascorbate also functions as an antioxidant in cosmetics at concentrations from 0.0003% to 0.3%. Related ingredients (Ascorbyl Palmitate, Ascorbyl Dipalmitate, Ascorbyl Stearate, Erythorbic Acid, and Sodium Erythorbate) have been previously reviewed by the Cosmetic Ingredient Review (CIR) Expert Panel and found "to be safe for use as cosmetic ingredients in the present practices of good use." Ascorbic Acid is a generally recognized as safe (GRAS) substance for use as a chemical preservative in foods and as a nutrient and/or dietary supplement. Calcium Ascorbate and Sodium Ascorbate are listed as GRAS substances for use as chemical preservatives. L-Ascorbic Acid is readily and reversibly oxidized to L-dehydroascorbic acid and both forms exist in equilibrium in the body. Permeation rates of Ascorbic Acid through whole and stripped mouse skin were 3.43 +/- 0.74 microg/cm(2)/h and 33.2 +/- 5.2 microg/cm(2)/h. Acute oral and parenteral studies in mice, rats, rabbits, guinea pigs, dogs, and cats demonstrated little toxicity. Ascorbic Acid and Sodium Ascorbate acted as a nitrosation inhibitor in several food and cosmetic product studies. No compound-related clinical signs or gross or microscopic pathological effects were

  10. Improved Outcomes With Retinoic Acid and Arsenic Trioxide Compared With Retinoic Acid and Chemotherapy in Non-High-Risk Acute Promyelocytic Leukemia: Final Results of the Randomized Italian-German APL0406 Trial.

    Science.gov (United States)

    Platzbecker, Uwe; Avvisati, Giuseppe; Cicconi, Laura; Thiede, Christian; Paoloni, Francesca; Vignetti, Marco; Ferrara, Felicetto; Divona, Mariadomenica; Albano, Francesco; Efficace, Fabio; Fazi, Paola; Sborgia, Marco; Di Bona, Eros; Breccia, Massimo; Borlenghi, Erika; Cairoli, Roberto; Rambaldi, Alessandro; Melillo, Lorella; La Nasa, Giorgio; Fiedler, Walter; Brossart, Peter; Hertenstein, Bernd; Salih, Helmut R; Wattad, Mohammed; Lübbert, Michael; Brandts, Christian H; Hänel, Mathias; Röllig, Christoph; Schmitz, Norbert; Link, Hartmut; Frairia, Chiara; Pogliani, Enrico Maria; Fozza, Claudio; D'Arco, Alfonso Maria; Di Renzo, Nicola; Cortelezzi, Agostino; Fabbiano, Francesco; Döhner, Konstanze; Ganser, Arnold; Döhner, Hartmut; Amadori, Sergio; Mandelli, Franco; Ehninger, Gerhard; Schlenk, Richard F; Lo-Coco, Francesco

    2017-02-20

    Purpose The initial results of the APL0406 trial showed that the combination of all- trans-retinoic acid (ATRA) and arsenic trioxide (ATO) is at least not inferior to standard ATRA and chemotherapy (CHT) in first-line therapy of low- or intermediate-risk acute promyelocytic leukemia (APL). We herein report the final analysis on the complete series of patients enrolled onto this trial. Patients and Methods The APL0406 study was a prospective, randomized, multicenter, open-label, phase III noninferiority trial. Eligible patients were adults between 18 and 71 years of age with newly diagnosed, low- or intermediate-risk APL (WBC at diagnosis ≤ 10 × 10(9)/L). Overall, 276 patients were randomly assigned to receive ATRA-ATO or ATRA-CHT between October 2007 and January 2013. Results Of 263 patients evaluable for response to induction, 127 (100%) of 127 patients and 132 (97%) of 136 patients achieved complete remission (CR) in the ATRA-ATO and ATRA-CHT arms, respectively ( P = .12). After a median follow-up of 40.6 months, the event-free survival, cumulative incidence of relapse, and overall survival at 50 months for patients in the ATRA-ATO versus ATRA-CHT arms were 97.3% v 80%, 1.9% v 13.9%, and 99.2% v 92.6%, respectively ( P < .001, P = .0013, and P = .0073, respectively). Postinduction events included two relapses and one death in CR in the ATRA-ATO arm and two instances of molecular resistance after third consolidation, 15 relapses, and five deaths in CR in the ATRA-CHT arm. Two patients in the ATRA-CHT arm developed a therapy-related myeloid neoplasm. Conclusion These results show that the advantages of ATRA-ATO over ATRA-CHT increase over time and that there is significantly greater and more sustained antileukemic efficacy of ATO-ATRA compared with ATRA-CHT in low- and intermediate-risk APL.

  11. Multiattribute Utility Theory without Expected Utility Foundations

    NARCIS (Netherlands)

    A.M. Stiggelbout; P.P. Wakker

    1995-01-01

    Methods for determining the form of utilities are needed for the implementation of utility theory in specific decisions. An important step forward was achieved when utility theorists characterized useful parametric families of utilities, and simplifying decompositions of multiattribute utilities. Th

  12. 柠檬酸在改善日粮磷利用率中的应用%Application of Citric Acid Improving Phosphorus Utilization in Diet

    Institute of Scientific and Technical Information of China (English)

    刘瑞玲

    2011-01-01

    柠檬酸作为一种重要的饲料酸化剂,可以降低胃肠道pH,提高消化酶活性,改善胃肠道微生物区系,促进矿物质和维生素的吸收及消除抗营养因子。柠檬酸制剂能与磷形成一种生物效价高的络合物,促进磷的吸收和保留,可降低饲料的含磷量,有效地减少动物粪便中磷的排出。文章综述了植酸的抗营养作用、柠檬酸的作用及其在畜禽生产中的作用。%Citric acid is an important agent, it could reduce the acidi fication of the feed gastrointestinal pH value, improve the digestive enzymes activity, improve the gastrointestinal microbial zone, promote the minerals and vitamins absorption and eliminate antinutritional factors functions. Citric acid preparations and phosphorus can form a kind of prices, it had higher biological effection, and promoted the phosphorus to be absorbed of and withholded, so the phosphorus content of be greatly reduced, effectively reduce phosphorus eduction in animal feces. This paper reviewed the anti-nutritive effect of phytie acid, the effect of citric acid and its application in animal production.

  13. Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on the nitrogen utilization in growing pigs

    NARCIS (Netherlands)

    Lenis, N.P.; Bikker, P.; Meulen, van der J.; Diepen, van J.Th.M.; Bakker, J.G.M.; Jongbloed, A.W.

    1996-01-01

    Two experiments were conducted to determine the effects of dietary NDF on apparent ileal and fecal digestibility and portal flux of nitrogen (N) and amino acids, and on N retention in growing pigs. In four equal portions (at 0600, 1200, 1800, and 2400) barrows on Treatment B received a basal diet, b

  14. Ocular pharmacokinetics of acyclovir amino acid ester prodrugs in the anterior chamber: evaluation of their utility in treating ocular HSV infections.

    Science.gov (United States)

    Katragadda, Suresh; Gunda, Sriram; Hariharan, Sudharshan; Mitra, Ashim K

    2008-07-09

    To evaluate in vivo corneal absorption of the amino acid prodrugs of acyclovir (ACV) using a topical well model and microdialysis in rabbits. Stability of L-alanine-ACV (AACV), L-serine-ACV (SACV), L-isoleucine-ACV (IACV), gamma-glutamate-ACV (EACV) and L-valine-ACV (VACV) prodrugs was evaluated in various ocular tissues. Dose-dependent toxicity of these prodrugs was also examined in rabbit primary corneal epithelial cell culture (rPCEC) using 96-well based cell proliferation assay. In vivo ocular bioavailability of these compounds was also evaluated with a combination of topical well infusion and aqueous humor microdialysis techniques. Among the amino acid ester prodrugs, SACV was most stable in aqueous humor. Enzymatic degradation of EACV was the least compared to all other prodrugs. Cellular toxicity of all the prodrugs was significantly less compared to trifluorothymidine (TFT) at 5mM. Absorption rate constants of all the compounds were found to be lower than the elimination rate constants. All the prodrugs showed similar terminal elimination rate constants (lambda(z)). SACV and VACV exhibited approximately two-fold increase in area under the curve (AUC) relative to ACV (pACV, respectively. Amino acid ester prodrugs of ACV were absorbed through the cornea at varying rates (ka) thereby leading to varying extents (AUC). The amino acid ester prodrug, SACV owing to its enhanced stability, comparable AUC and high concentration at last time point (Clast) seems to be a promising candidate for the treatment of ocular HSV infections.

  15. Monitoring utilizations of amino acids and vitamins in culture media and Chinese hamster ovary cells by liquid chromatography tandem mass spectrometry.

    Science.gov (United States)

    Qiu, Jinshu; Chan, Pik Kay; Bondarenko, Pavel V

    2016-01-01

    Monitoring amino acids and vitamins is important for understanding human health, food nutrition and the culture of mammalian cells used to produce therapeutic proteins in biotechnology. A method including ion pairing reversed-phase liquid chromatography with tandem mass spectrometry was developed and optimized to quantify 21 amino acids and 9 water-soluble vitamins in Chinese hamster ovary (CHO) cells and culture media. By optimizing the chromatographic separation, scan time, monitoring time window, and sample preparation procedure, and using isotopically labeled (13)C, (15)N and (2)H internal standards, low limits of quantitation (≤0.054 mg/L), good precision (amino acids showed a zigzag pattern with maxima at the feeding days, and 9 non-essential amino acids displayed a smoothly changing profile as they were mainly products of cellular metabolism. Five of 9 vitamins accumulated continuously during the culture period, suggesting that they were fed in access. The method serves as an effective tool for the development and optimization of mammalian cell cultures.

  16. Monitoring and control requirement definition study for dispersed storage and generation (DSG). Volume IV. Final report, Appendix C: identification from utility visits of present and future approaches to integration of DSG into distribution networks

    Energy Technology Data Exchange (ETDEWEB)

    1980-10-01

    A major aim of the US National Energy Policy, as well as that of the New York State Energy Research and Development Authority, is to conserve energy and to shift from oil to more abundant domestic fuels and renewable energy sources. Dispersed Storage and Generation (DSG) is the term that characterizes the present and future dispersed, relatively small (<30 MW) energy systems, such as solar thermal electric, photovoltaic, wind, fuel cell, storage battery, hydro, and cogeneration, which can help achieve these national energy goals and can be dispersed throughout the distribution portion of an electric utility system. As a result of visits to four utilities concerned with the use of DSG power sources on their distribution networks, some useful impressions of present and future approaches to the integration of DSGs into electrical distribution network have been obtained. A more extensive communications and control network will be developed by utilities for control of such sources for future use. Different approaches to future utility systems with DSG are beginning to take shape. The new DSG sources will be in decentralized locations with some measure of centralized control. The utilities have yet to establish firmly the communication and control means or their organization. For the present, the means for integrating the DSGs and their associated monitoring and control equipment into a unified system have not been decided.

  17. Effect of rate of weight gain of steers during the stocker phase. IV. Rumen fermentation characteristics and expression of genes involved in substrate utilization for fatty acid synthesis in adipose tissues of growing-finishing beef cattle.

    Science.gov (United States)

    Lancaster, P A; Sharman, E D; Horn, G W; Krehbiel, C R; Dillwith, J W; Starkey, J D

    2015-06-01

    The objective of this study was to determine the impact of stocker production systems differing in growth rate on rumen fermentation characteristics and utilization of substrates for fatty acid synthesis in intramuscular (IM), subcutaneous (SC), and perirenal (PR) adipose tissues. Angus steers were assigned to 4 stocker cattle production systems in 2 consecutive years: 1) 1.0 kg/d of 40% CP cottonseed meal–based supplement while grazing dormant native range (CON), 2) ground corn/soybean meal–based supplement while grazing dormant native range fed at 1% of BW (CORN), 3) grazing wheat pasture at a high stocking rate to achieve a low rate of BW gain (LGWP), and 4) grazing wheat pasture at a low stocking rate for a high rate of BW gain (HGWP). Eight ruminally cannulated steers were used to determine rumen fermentation characteristics. Steers were harvested during the stocker phase at similar age (different carcass weight) in Exp. 1 (3 steers/treatment) or at similar carcass weight in Exp. 2 (4 steers/treatment). Adipose tissues were analyzed for mRNA expression of genes involved in glucose (solute carrier family 2, member 4 [GLUT4], glucose-6-phosphate dehydrogenase [G6PDH], phosphofructokinase, muscle [PFKM], and pyruvate kinase 2, muscle [PK2]), lactate (lactate dehydrogenase B [LDHB]), and acetate (acetyl-CoA synthetase, cytosol [ACSS2]) utilization for fatty acid synthesis. The acetate:propionate ratio was least (P adipose tissue. Expression of PFKM and PK2 mRNA tended (F-test; P adipose tissue. At similar HCW, expression of GLUT4 and G6PDH mRNA were greater (P adipose tissue of LGWP and HGWP steers compared with CON and CORN steers but not in IM and PR adipose tissue. Expression of LDHB mRNA was lesser (P adipose tissue but greater (P adipose tissue of LGWP and HGWP steers compared with CON and CORN steers. These results indicate a shift toward glucose utilization in SC adipose tissue but a shift towards lactate utilization in PR adipose tissue. These results

  18. Medical devices; immunology and microbiology devices; classification of multiplex nucleic acid assay for identification of microorganisms and resistance markers from positive blood cultures. Final order.

    Science.gov (United States)

    2015-05-27

    The Food and Drug Administration (FDA) is classifying multiplex nucleic acid assay for identification of microorganisms and resistance markers from positive blood cultures into class II (special controls). The special controls that will apply to this device are identified in this order and will be part of the codified language for the multiplex nucleic acid assay for identification of microorganisms and resistance markers from positive blood cultures. The Agency is classifying the device into class II (special controls) in order to provide a reasonable assurance of safety and effectiveness of the device.

  19. Determination of Labeled Fatty Acids Content in Milk Products, Infant Formula, and Adult/Pediatric Nutritional Formula by Capillary Gas Chromatography: Collaborative Study, Final Action 2012.13.

    Science.gov (United States)

    Golay, Pierre-Alain; Moulin, Julie

    2016-01-01

    A collaborative study was conducted on AOAC First Action Method 2012.13 "Determination of Labeled Fatty Acids Content in Milk Products and Infant Formula by Capillary Gas Chromatography," which is based on an initial International Organization for Standardization (ISO)-International Dairy Federation (IDF) New Work Item that has been moved forward to ISO 16958:2015|IDF 231:2015 in November 2015. It was decided to merge the two activities after the agreement signed between ISO and AOAC in June 2012 to develop common standards and to avoid duplicate work. The collaborative study was performed after having provided highly satisfactory single-laboratory validation results [Golay, P.A., & Dong, Y. (2015) J. AOAC Int. 98, 1679-1696] that exceeded the performance criteria defined in AOAC Standard Method Performance Requirement (SMPR(®)) 2012.011 (September 29, 2012) on 12 products selected by the AOAC Stakeholder Panel on Infant Formula (SPIFAN). After a qualification period of 1 month, 18 laboratories participated in the fatty acids analysis of 12 different samples in duplicate. Six samples were selected to meet AOAC SPIFAN requirements (i.e., infant formula and adult nutritionals in powder and liquid formats), and the other Six samples were selected to meet ISO-IDF requirements (i.e., dairy products such as milk powder, liquid milk, cream, butter, infant formula with milk, and cheese). The fatty acids were analyzed directly in all samples without preliminary fat extraction, except in one sample (cheese). Powdered samples were analyzed after dissolution (i.e., reconstitution) in water, whereas liquid samples (or extracted fat) were analyzed directly. After addition of the internal standards solution [C11:0 fatty acid methyl ester (FAME) and C13:0 triacylglycerols (TAG)] to the samples, fatty acids attached to lipids were transformed into FAMEs by direct transesterification using methanolic sodium methoxide. FAMEs were separated using highly polar capillary GLC and were

  20. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gurney, Kevin R

    2015-01-12

    This document constitutes the final report under DOE grant DE-FG-08ER64649. The organization of this document is as follows: first, I will review the original scope of the proposed research. Second, I will present the current draft of a paper nearing submission to Nature Climate Change on the initial results of this funded effort. Finally, I will present the last phase of the research under this grant which has supported a Ph.D. student. To that end, I will present the graduate student’s proposed research, a portion of which is completed and reflected in the paper nearing submission. This final work phase will be completed in the next 12 months. This final workphase will likely result in 1-2 additional publications and we consider the results (as exemplified by the current paper) high quality. The continuing results will acknowledge the funding provided by DOE grant DE-FG-08ER64649.

  1. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    DeTar, Carleton [P.I.

    2012-12-10

    This document constitutes the Final Report for award DE-FC02-06ER41446 as required by the Office of Science. It summarizes accomplishments and provides copies of scientific publications with significant contribution from this award.

  2. A Comparative Study of the Quality of Apical Seal in Resilon/Epiphany SE Following Intra canal Irrigation With 17% EDTA, 10% Citric Acid, And MTAD as Final Irrigants - A Dye Leakage Study Under Vacuum.

    Science.gov (United States)

    Balasubramanian, Saravana Karthikeyan; Saraswathi, Vidya; Ballal, Nidambur Vasudev; Acharya, Shashi Rashmi; Sampath, J Sivakumar; Singh, Sandeep

    2017-02-01

    Adequate apical sealing ability of the root canal filling material is an essential requisite for a successful endodontic therapy. Various endodontic irrigants are used for the removal of smear layer before obturating with a solid core material, thereby, reducing microleakage and improving apical seal. Resilon, a synthetic material was developed as an alternative to replace the conventional gutta-percha (standard root canal filling material) and traditional sealers for the obturation of endodontically treated teeth. To evaluate and compare in-vitro, the post obturation apical seal obtained with Resilon /Epiphany SE (Self Etch) sealer following irrigation with 17% Ethylenediamine Tetra-Acetic Acid (EDTA), 10% citric acid, and MTAD (a mixture of doxycycline, citric acid, and a detergent, Tween 80), as final irrigants in combination with Sodium hypochlorite (NaOCl) using dye leakage under vacuum method. Fifty five single rooted human maxillary central incisors were subjected to root canal instrumentation. Based on the final irrigation solution, samples were divided into three experimental groups (n=15); (I) 17% EDTA + 1.3% NaOCl, (II) 10% citric acid + 1.3% NaOCl, (III) MTAD + 1.3% NaOCl and two control groups (positive and negative) with 0.9% normal saline as a final irrigant. The samples were obturated with resilon/epiphany SE sealer according to manufacturer instructions and placed in 2% rhodamine B dye solution under vacuum pressure for 30 minutes and allowed to remain in the dye for seven days. All samples were then longitudinally split and examined for dye leakage under stereomicroscope and the data were statistically analysed using one-way ANOVA and post hoc tukey test. Statistically significant difference (p=0.001) was observed in the mean apical leakage between the experimental and the control groups. However, there was no significant difference (p>0.05) observed in the mean apical leakage amongst the three experimental groups. 17% EDTA, 10% citric acid, and

  3. A Comparative Study of the Quality of Apical Seal in Resilon/Epiphany SE Following Intra canal Irrigation With 17% EDTA, 10% Citric Acid, And MTAD as Final Irrigants – A Dye Leakage Study Under Vacuum

    Science.gov (United States)

    Saraswathi, Vidya; Ballal, Nidambur Vasudev; Acharya, Shashi Rashmi; Sampath, J. Sivakumar; Singh, Sandeep

    2017-01-01

    Introduction Adequate apical sealing ability of the root canal filling material is an essential requisite for a successful endodontic therapy. Various endodontic irrigants are used for the removal of smear layer before obturating with a solid core material, thereby, reducing microleakage and improving apical seal. Resilon, a synthetic material was developed as an alternative to replace the conventional gutta-percha (standard root canal filling material) and traditional sealers for the obturation of endodontically treated teeth. Aim To evaluate and compare in-vitro, the post obturation apical seal obtained with Resilon /Epiphany SE (Self Etch) sealer following irrigation with 17% Ethylenediamine Tetra-Acetic Acid (EDTA), 10% citric acid, and MTAD (a mixture of doxycycline, citric acid, and a detergent, Tween 80), as final irrigants in combination with Sodium hypochlorite (NaOCl) using dye leakage under vacuum method. Materials and Methods Fifty five single rooted human maxillary central incisors were subjected to root canal instrumentation. Based on the final irrigation solution, samples were divided into three experimental groups (n=15); (I) 17% EDTA + 1.3% NaOCl, (II) 10% citric acid + 1.3% NaOCl, (III) MTAD + 1.3% NaOCl and two control groups (positive and negative) with 0.9% normal saline as a final irrigant. The samples were obturated with resilon/epiphany SE sealer according to manufacturer instructions and placed in 2% rhodamine B dye solution under vacuum pressure for 30 minutes and allowed to remain in the dye for seven days. All samples were then longitudinally split and examined for dye leakage under stereomicroscope and the data were statistically analysed using one-way ANOVA and post hoc tukey test. Results Statistically significant difference (p=0.001) was observed in the mean apical leakage between the experimental and the control groups. However, there was no significant difference (p>0.05) observed in the mean apical leakage amongst the three

  4. Process Design Report for Wood Feedstock: Lignocellulosic Biomass to Ethanol Process Desing and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis Current and Futuristic Scenarios

    Energy Technology Data Exchange (ETDEWEB)

    Wooley, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States); Ruth, Mark [National Renewable Energy Lab. (NREL), Golden, CO (United States); Sheehan, John [National Renewable Energy Lab. (NREL), Golden, CO (United States); Ibsen, Kelly [National Renewable Energy Lab. (NREL), Golden, CO (United States); Majdeski, Henry [Delta-T Corporation, Lexington, KY (United States); Galves, Adrian [Delta-T Corporation, Lexington, KY (United States)

    1999-07-01

    The National Renewable Energy Laboratory (NREL) has undertaken a complete review and update of the process design and economic model for the biomass-to-ethanol process based on co-current dilute acid prehydrolysis, along with simultaneous saccharification (enzymatic) and co-fermentation. The process design includes the core technologies being researched by the U.S. Department of Energy (DOE): prehydrolysis, simultaneous saccharification and co-fermentation, and cellulase enzyme production.

  5. Efficient carbon dioxide utilization and simultaneous hydrogen enrichment from off-gas of acetone-butanol-ethanol fermentation by succinic acid producing Escherichia coli.

    Science.gov (United States)

    He, Aiyong; Kong, Xiangping; Wang, Chao; Wu, Hao; Jiang, Min; Ma, Jiangfeng; Ouyang, Pingkai

    2016-08-01

    The off-gas from acetone-butanol-ethanol (ABE) fermentation was firstly used to be CO2 source (co-substrate) for succinic acid production. The optimum ratio of H2/CO2 indicated higher CO2 partial pressures with presence of H2 could enhance C4 pathway flux and reductive product productivity. Moreover, when an inner recycling bioreactor was used for CO2 recycling at a high total pressure (0.2Mpa), a maximum succinic acid concentration of 65.7g·L(-1) was obtained, and a productivity of 0.76g·L(-1)·h(-1) and a high yield of 0.86g·g(-1) glucose were achieved. Furthermore, the hydrogen content was simultaneously enriched to 92.7%. These results showed one successful attempt to reuse the off-gas of ABE fermentation which can be an attractive CO2 source for succinic acid production. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Utilizing a nano-sorbent for the selective solid-phase extraction of vanillic acid prior to its determination by photoluminescence spectroscopy.

    Science.gov (United States)

    Abdolmohammad-Zadeh, Hossein; Mahmoudi-Kordi, Fatemeh; Rahmati, Manoochehr

    2014-12-01

    Vanillic acid (VA) is a phenolic acid, and acts as a natural antioxidant in fruits, vegetables and plants. The extraction and determination of trace levels of VA in plants is important, because stimulation of protein synthesis and activation of antioxidant enzymes occur in the presence of phenolic acids at trace levels. In this research, a photoluminescence spectroscopic method was developed for the quantification of VA in plant samples after separation and pre-concentration. Selective extraction of VA from aqueous solution was performed using a solid-phase extraction column packed with nickel-aluminum layered double hydroxide as a nano-sorbent. After elution of extracted analyte from the column using 3 mL of a 3 mol/L NaOH solution, its concentration was determined spectrofluorometrically at λ(em) = 357 nm with excitation at λ(ex) = 280 nm. The spectrofluorometry method gave a linear response for VA within the range 20.0-900.0 µg/L, with a correlation coefficient of 0.9982. The limit of detection and sorption capacity were 7.6 µg/L and 66.2 mg/g, respectively. The method was validated by comparing the obtained results with gas chromatographic data. This method was used to determine VA in Chenopodium album and Prangos asperula plants.

  7. Preparation and utilization of amorphous siliceous materials from serpentine (Mg3Si2O5(OH)4) by acid treatment; Jamonseki no kofuka kachika ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-01-30

    Concerning the conversion of serpentine, not only its magnesium component but also silica component, into industrial materials, conditions suitable for the production of porous materials and amorphous silica by acid treatment were evaluated, and the properties of the products were evaluated. The silica resulting from the acid treatment of serpentine comes out in different forms, each reflecting the structure of the parent rock, that is, an amorphous mass of planar particles from antigorite and a fascicular mass of filaments from chrysotile. A microporic structure resulted when a small quantity of magnesium was allowed to remain in the skeleton structure and acid treatment conditions were properly adjusted. Several siliceous compounds were prepared for the purpose of finding use for silica from this rock, and then it was found that high-efficiency production of high-crystallinity compounds was possible and that they were furnished with properties fit for use as materials. Furthermore, study was made about the kaolinite reaction in which serpentine would be directly converted into useful materials. 105 refs., 55 figs., 6 tabs.

  8. Reduction in database search space by utilization of amino acid composition information from electron transfer dissociation and higher-energy collisional dissociation mass spectra.

    Science.gov (United States)

    Hansen, Thomas A; Kryuchkov, Fedor; Kjeldsen, Frank

    2012-08-07

    With high-mass accuracy and consecutively obtained electron transfer dissociation (ETD) and higher-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS), reliable (≥97%) and sensitive fragment ions have been extracted for identification of specific amino acid residues in peptide sequences. The analytical benefit of these specific amino acid composition (AAC) ions is to restrict the database search space and provide identification of peptides with higher confidence and reduced false negative rates. The 6706 uniquely identified peptide sequences determined with a conservative Mascot score of >30 were used to characterize the AAC ions. The loss of amino acid side chains (small neutral losses, SNLs) from the charge reduced peptide radical cations was studied using ETD. Complementary AAC information from HCD spectra was provided by immonium ions. From the ETD/HCD mass spectra, 5162 and 6720 reliable SNLs and immonium ions were successfully extracted, respectively. Automated application of the AAC information during database searching resulted in an average 3.5-fold higher confidence level of peptide identification. In addition, 4% and 28% more peptides were identified above the significance level in a standard and extended search space, respectively.

  9. Community energy systems and the law of public utilities. Volume thirty-eight. Oklahoma. Final report of a study of the impacts of regulations affecting the acceptance of integrated community energy systems

    Energy Technology Data Exchange (ETDEWEB)

    Feurer, D.A.; Weaver, C.L.

    1981-01-01

    A detailed description is given of the laws and programs of the State of Oklahoma governing the regulation of public energy utilities, the siting of energy generating and transmission facilities, the municipal franchising of public energy utilities, and the prescription of rates to be charged by utilities including attendant problems of cost allocations, rate base and operating expense determinations, and rate of return allowances. These laws and programs are analyzed to identify impediments which they may present to the implementation of Integrated Community Energy Systems (ICES). This report is one of fifty-one separate volumes which describe such regulatory programs at the Federal level and in each state as background to the report entitled Community Energy Systems and the Law of Public Utilities, Volume One: An Overview. This report also contains a summary of a strategy described in Volume One: An Overview for overcoming these impediments by working within the existing regulatory framework and by making changes in the regulatory programs to enhance the likelihood of ICES implementation.

  10. Preparation and Characteristics of Polyaluminium Chloride by Utilizing Fluorine-Containing Waste Acidic Mother Liquid from Clay-Brine Synthetic Cryolite Process

    Directory of Open Access Journals (Sweden)

    Feng-shan Zhou

    2014-01-01

    Full Text Available Clay-brine process employing activated clay, NaCl, HCl, and HF as raw materials is the primarily advanced technology to synthesize cryolite in the present industrial grade. However, plenty of byproducts of fluorine-containing waste HCl at the concentration of about 10%~12% could not be utilized comprehensively and are even hazardous to the environment. This work proposed a new two-step technology to prepare inorganic polymer flocculants polyaluminium chloride (PAC from synthetic cryolite mother liquor. Many specific factors such as the variety of aluminide source, reaction temperature and time, reagent ratio, and manner of alkaline addition were taken into consideration and their influences on the performances of produced PAC were discussed. It was found that synthetic cryolite mother liquor could react with bauxite and calcium aluminate directly to prepare cheap PAC, with plenty amount of water insoluble CaF2 and CaSiF6 produced as well. However, once HCl was introduced into synthetic cryolite mother liquor as well as by utilizing bauxite as aluminide source and sodium aluminate as adjusting basicity agent, the resultant PAC would dissolve out higher amount of aluminum while producing little amount of water insoluble materials. The coagulation behavior of the specially produced PAC could even match the industrial grade PAC conforming to national standard.

  11. Label-free and ratiometric detection of nuclei acids based on graphene quantum dots utilizing cascade amplification by nicking endonuclease and catalytic G-quadruplex DNAzyme.

    Science.gov (United States)

    Wang, Guang-Li; Fang, Xin; Wu, Xiu-Ming; Hu, Xue-Lian; Li, Zai-Jun

    2016-07-15

    Herein, we report a ratiometric fluorescence assay based on graphene quantum dots (GQDs) for the ultrasensitive DNA detection by coupling the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for cascade signal amplifications. With o-phenylenediamine acted as the substrate of G-quadruplex/hemin DNAzyme, whose oxidization product (that is, 2,3-diaminophenazine, DAP) quenched the fluorescence intensity of GQDs (at 460nm) obviously, accompanied with the emergence of a new emission of DAP (at 564nm). The ratiometric signal variations at the emission wavelengths of 564 and 460nm (I564/I460) were utilized for label-free, sensitive, and selective detection of target DNA. Utilizing the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for amplified cascade generation of DAP, the proposed bioassay exhibited high sensitivity toward target DNA with a detection limit of 30fM. The method also had additional advantages such as facile preparation and easy operation.

  12. Final report on the safety assessment of Glycyrrhetinic Acid, Potassium Glycyrrhetinate, Disodium Succinoyl Glycyrrhetinate, Glyceryl Glycyrrhetinate, Glycyrrhetinyl Stearate, Stearyl Glycyrrhetinate, Glycyrrhizic Acid, Ammonium Glycyrrhizate, Dipotassium Glycyrrhizate, Disodium Glycyrrhizate, Trisodium Glycyrrhizate, Methyl Glycyrrhizate, and Potassium Glycyrrhizinate.

    Science.gov (United States)

    2007-01-01

    Glycyrrhetinic Acid and its salts and esters and Glycyrrhizic Acid and its salts and esters are cosmetic ingredients that function as flavoring agents or skin-conditioning agents - miscellaneous or both. These chemicals may be isolated from licorice plants. Glycyrrhetinc Acid is described as at least 98% pure, with 0.6% 24-OH-Glycyrrhetinic Acid, not more than 20 mu g/g of heavy metals and not more than 2 mu g/g of arsenic. Ammonium Glycyrrhizate has been found to be at least 98% pure and Dipotassium Glycyrrhizate has been found to be at least 95% pure. Glycyrrhetinic Acid is used in cosmetics at concentrations of up to 2%; Stearyl Glycyrrhetinate, up to 1%; Glycyrrhizic Acid, up to 0.1%; Ammonium Glycyrrhizate, up to 5%; Dipotassium Glycyrrhizate, up to 1%; and Potassium Glycyrretinate, up to 1%. Although Glycyrrhizic Acid is poorly absorbed by the intestinal tract, it may be hydrolyzed to Glycyrrhetinic Acid by a beta -glucuronidase produced by intestinal bacteria. Glycyrrhetinic Acid and Glycyrrhizic Acid bind to rat and human albumin, but do not absorb well into tissues. Glycyrrhetinic Acid and Glycyrrhizic Acid and metabolites are mostly excreted in the bile, with very little excreted in urine. Dipotassium Glycyrrhizate was undetectable in the receptor chamber when tested for transepidermal permeation through pig skin. Glycyrrhizic Acid increased the dermal penetration of diclofenac sodium in rat skin. Dipotassium Glycyrrhizate increased the intestinal absorption of calcitonin in rats. In humans, Glycyrrhetinic Acid potentiated the effects of hydrocortisone in the skin. Moderate chronic or high acute exposure to Glycyrrhizic Acid, Ammonium Glycyrrhizate, and their metabolites have been demonstrated to cause transient systemic alterations, including increased potassium excretion, sodium and water retention, body weight gain, alkalosis, suppression of the renin-angiotensis-aldosterone system, hypertension, and muscular paralysis; possibly through inhibition of

  13. Exceptional gravimetric and volumetric CO2 uptake in a palladated NbO-type MOF utilizing cooperative acidic and basic, metal-CO2 interactions.

    Science.gov (United States)

    Spanopoulos, I; Bratsos, I; Tampaxis, C; Vourloumis, D; Klontzas, E; Froudakis, G E; Charalambopoulou, G; Steriotis, T A; Trikalitis, P N

    2016-08-18

    A novel NbO-type MOF is reported based on a palladated organic linker, showing a remarkable gravimetric and volumetric CO2 uptake, reaching 201.8 cm(3) g(-1) (9.0 mmol g(-1), 39.7 wt%) and 187.8 cm(3) cm(-3) at 273 K and 1 bar, respectively. Accurate theoretical calculations revealed that the exceptional CO2 uptake is due to the combination of Lewis base Pd(ii)-CO2 (24.3 kJ mol(-1)) and Lewis acid Cu(ii)-CO2 (30.3 kJ mol(-1)) interactions, as well as synergistic pore size effects.

  14. Glucagon-like peptide-2 (GLP-2) increases net amino acid utilization by the portal-drained viscera of ruminating calves

    DEFF Research Database (Denmark)

    Taylor-Edwards, C C; Burrin, D G; Kristensen, Niels Bastian

    2012-01-01

    Glucagon-like peptide-2 (GLP-2) increases small intestinal mass and blood flow in ruminant calves, but its impact on nutrient metabolism across the portal-drained viscera (PDV) and liver is unknown. Eight Holstein calves with catheters in the carotid artery, mesenteric vein, portal vein and hepatic...... on days 0 and 10. Arterial concentrations and net fluxes of all amino acids and glucose metabolism using continuous intravenous infusion of [U13-C]glucose were measured on day 10 only. A 1-h infusion of GLP-2 increased blood flow in the portal and hepatic veins when administered to calves not previously...

  15. Research and development of a phosphoric acid fuel cell/battery power source integrated in a test-bed bus. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-05-30

    This project, the research and development of a phosphoric acid fuel cell/battery power source integrated into test-bed buses, began as a multi-phase U.S. Department of Energy (DOE) project in 1989. Phase I had a goal of developing two competing half-scale (25 kW) brassboard phosphoric acid fuel cell systems. An air-cooled and a liquid-cooled fuel cell system were developed and tested to verify the concept of using a fuel cell and a battery in a hybrid configuration wherein the fuel cell supplies the average power required for operating the vehicle and a battery supplies the `surge` or excess power required for acceleration and hill-climbing. Work done in Phase I determined that the liquid-cooled system offered higher efficiency.

  16. Reclamation of acid, toxic coal spoils using wet flue gas desulfurization by-product, fly ash and sewage sludge. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Kost, D.A.; Vimmerstedt, J.P.; Stehouwer, R.C.

    1997-03-01

    Establishment of vegetation on acid abandoned minelands requires modification of soil physical and chemical conditions. Covering the acid minesoil with topsoil or borrow soil is a common practice but this method may be restricted by availability of borrow soil and cause damage to the borrow site. An alternative approach is to use waste materials as soil amendments. There is a long history of using sewage sludge and fly ash as amendments for acid minesoils. Flue gas desulfurization (FGD) by-products are newer materials that are also promising amendments. Most flue gas sludges are mixtures of Calcium sulfate (CaSO{sub 4}), calcium sulfite (CaSO{sub 3}), calcium carbonate (CaCO{sub 3}), calcium hydroxide [Ca(OH){sub 2}], and fly ash. Some scrubbing processes produce almost pure gypsum (CaSO{sub 4}2H{sub 2}O). The primary purpose of the project is to evaluate two wet FGD by-products for effects on vegetation establishment and surface and ground water quality on an acid minesoil. One by-product from the Conesville, OH power plant (American Electric Power Service Corporation) contains primarily calcium sulfite and fly ash. The other by-product (Mg-gypsum FGD) from an experimental scrubber at the Zimmer power plant (Cincinnati Gas and Electric Company) is primarily gypsum with 4% magnesium hydroxide. These materials were compared with borrow soil and sewage sludge as minesoil amendments. Combinations of each FGD sludge with sewage sludge were also tested. This report summarizes two years of measurements of chemical composition of runoff water, ground water at two depths in the subsoil, soil chemical properties, elemental composition and yield of herbaceous ground cover, and elemental composition, survival and height of trees planted on plots treated with the various amendments. The borrow soil is the control for comparison with the other treatments.

  17. Utilization of stable isotopes for the study of in vivo compartmental metabolism of poly-insaturate fatty acids; Utilisation des isotopes stables pour l`etude du metabolisme compartimental in vivo d`acides gras polyinsatures

    Energy Technology Data Exchange (ETDEWEB)

    Brossard, N.; Croset, M.; Lecerf, J.; Lagarde, M. [Institut National des Sciences Appliquees (INSA), 69 - Villeurbanne (France); Pachiaudi, C.; Normand, S.; Riou, J.P. [Faculte de Medecine, 69 - Lyon (France); Chirouze, V.; Tayot, J.L. [IMEDEX, 69 - Chaponost (France)

    1994-12-31

    In order to study the compartmental metabolism of the 22:6n-3 fatty acid, and particularly the role of the transport plasmatic forms for the tissue uptake (especially brain), a technique is developed using carbon 13 stable isotope and an isotopic mass spectrometry coupled to gaseous chromatography technique. This method has been validated in rat with docosahexaenoic acid enriched in {sup 13}C and esterified in triglycerides. The compartmental metabolism is monitored by measuring the variation of 22:6n-3 isotopic enrichment in the various lipoprotein lipidic fractions, in blood globules and in the brain. 1 fig., 1 tab., 12 refs.

  18. Electric utility engineer`s FGD manual -- Volume 2: Major mechanical equipment; FGD proposal evaluations; Use of FGDPRISM in FGD system modification, proposal, evaluation, and design; FGD system case study. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-04

    Part 2 of this manual provides the electric utility engineer with detailed technical information on some of the major mechanical equipment used in the FGD system. The objectives of Part 2 are the following: to provide the electric utility engineer with information on equipment that may be unfamiliar to him, including ball mills, vacuum filters, and mist eliminators; and to identify the unique technique considerations imposed by an FGD system on more familiar electric utility equipment such as fans, gas dampers, piping, valves, and pumps. Part 3 provides an overview of the recommended procedures for evaluating proposals received from FGD system vendors. The objectives are to provide procedures for evaluating the technical aspects of proposals, and to provide procedures for determining the total costs of proposals considering both initial capital costs and annual operating and maintenance costs. The primary objective of Part 4 of this manual is to provide the utility engineer who has a special interest in the capabilities of FGDPRISM [Flue Gas Desulfurization PRocess Integration and Simulation Model] with more detailed discussions of its uses, requirements, and limitations. Part 5 is a case study in using this manual in the preparation of a purchase specification and in the evaluation of proposals received from vendors. The objectives are to demonstrate how the information contained in Parts 1 and 2 can be used to improve the technical content of an FGD system purchase specification; to demonstrate how the techniques presented in Part 3 can be used to evaluate proposals received in response to the purchase specification; and to illustrate how the FGDPRISM computer program can be used to establish design parameters for the specification and evaluate vendor designs.

  19. Utilization of fly ash to improve the quality of the acid mine drainage generated by oxidation of a sulphide-rich mining waste: Column experiments

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Lopez, R.; Nieto, J.M.; de Almodovar, G.R. [University of Huelva, Huelva (Spain). Dept. of Geology

    2007-04-15

    The production of Acid Mine Drainage (AMD) as a result of the oxidative dissolution of sulphides is one of the main pollution problems affecting natural watercourses in mining environments with sulphide-rich residues. In this work, the generation of AMD was prevented by means of the addition of fly ash to sulphide-rich residues in non-saturated column experiments. A column experiment filled with a pyrite-rich sludge with artificial irrigation leached acid drainages (pH approx. 2) containing high concentrations of sulphate, iron and other metals. However, non-saturated column experiments filled with pyritic-rich sludge and fly ash drained leachates characterized by alkaline pH (pH up to 10), low sulphate concentration, and lack of iron and other metals in solution. The pyrite oxidative dissolution at high pH, as a consequence of the leaching of fly ash, favours the metal precipitation inside the column (mainly iron), the coating of pyrite grains, and the attenuation of the oxidation process, resulting in a great improvement in the quality of the leachates.

  20. Determination of arsenic species in solid matrices utilizing supercritical fluid extraction coupled with gas chromatography after derivatization with thioglycolic acid n-butyl ester.

    Science.gov (United States)

    Wang, Zhifeng; Cui, Zhaojie

    2016-12-01

    A method using derivatization and supercritical fluid extraction coupled with gas chromatography was developed for the analysis of dimethylarsinate, monomethylarsonate and inorganic arsenic simultaneously in solid matrices. Thioglycolic acid n-butyl ester was used as a novel derivatizing reagent. A systematic discussion was made to investigate the effects of pressure, temperature, flow rate of the supercritical CO2 , extraction time, concentration of the modifier, and microemulsion on extraction efficiency. The application for real environmental samples was also studied. Results showed that thioglycolic acid n-butyl ester was an effective derivatizing reagent that could be applied for arsenic speciation. Using methanol as modifier of the supercritical CO2 can raise the extraction efficiency, which can be further enhanced by adding a microemulsion that contains Triton X-405. The optimum extraction conditions were: 25 MPa, 90°C, static extraction for 10 min, dynamic extraction for 25 min with a flow rate of 2.0 mL/min of supercritical CO2 modified by 5% v/v methanol and microemulsion. The detection limits of dimethylarsinate, monomethylarsonate, and inorganic arsenic in solid matrices were 0.12, 0.26, and 1.1 mg/kg, respectively. The optimized method was sensitive, convenient, and reliable for the extraction and analysis of different arsenic species in solid samples.

  1. Utilization of Boron Compounds for the Modification of Suberoyl Anilide Hydroxamic Acid as Inhibitor of Histone Deacetylase Class II Homo sapiens.

    Science.gov (United States)

    Bakri, Ridla; Parikesit, Arli Aditya; Satriyanto, Cipta Prio; Kerami, Djati; Tambunan, Usman Sumo Friend

    2014-01-01

    Histone deacetylase (HDAC) has a critical function in regulating gene expression. The inhibition of HDAC has developed as an interesting anticancer research area that targets biological processes such as cell cycle, apoptosis, and cell differentiation. In this study, an HDAC inhibitor that is available commercially, suberoyl anilide hydroxamic acid (SAHA), has been modified to improve its efficacy and reduce the side effects of the compound. Hydrophobic cap and zinc-binding group of these compounds were substituted with boron-based compounds, whereas the linker region was substituted with p-aminobenzoic acid. The molecular docking analysis resulted in 8 ligands with ΔG binding value more negative than the standards, SAHA and trichostatin A (TSA). That ligands were analyzed based on the nature of QSAR, pharmacological properties, and ADME-Tox. It is conducted to obtain a potent inhibitor of HDAC class II Homo sapiens. The screening process result gave one best ligand, Nova2 (513246-99-6), which was then further studied by molecular dynamics simulations.

  2. A mutation in the COX5 gene of the yeast Scheffersomyces stipitis alters utilization of amino acids as carbon source, ethanol formation and activity of cyanide insensitive respiration.

    Science.gov (United States)

    Freese, Stefan; Passoth, Volkmar; Klinner, Ulrich

    2011-04-01

    Scheffersomyces stipitis PJH was mutagenized by random integrative mutagenesis and the integrants were screened for lacking the ability to grow with glutamate as sole carbon source. One of the two isolated mutants was damaged in the COX5 gene, which encodes a subunit of the cytochrome c oxidase. BLAST searches in the genome of Sc. stipitis revealed that only one singular COX5 gene exists in Sc. stipitis, in contrast to Saccharomyces cerevisiae, where two homologous genes are present. Mutant cells had lost the ability to grow with the amino acids glutamate, proline or aspartate and other non-fermentable carbon sources, such as acetic acid and ethanol, as sole carbon sources. Biomass formation of the mutant cells in medium containing glucose or xylose as carbon source was lower compared with the wild-type cells. However, yields and specific ethanol formation of the mutant were much higher, especially under conditions of higher aeration. The mutant cells lacked both cytochrome c oxidase activity and cyanide-sensitive respiration, whereas ADH and PDC activities were distinctly enhanced. SHAM-sensitive respiration was obviously essential for the fermentative metabolism, because SHAM completely abolished growth of the mutant cells with both glucose or xylose as carbon source.

  3. Photovoltaic concentrator application experiment, Phase I: a 150 KW photovoltaic concentrator power system for load-center applications with feedback into the utility grid. Final report, June 1, 1978--March 31, 1979

    Energy Technology Data Exchange (ETDEWEB)

    Noel, G T; Alexander, G; Stember, L H; Stickford, G H; Smail, H E; Broehl, J H; Carmichael, D C

    1979-04-01

    A 150-kW-peak concentrator-type photovoltaic power system to supply a multiple building load application in the Columbus, Ohio area was designed and analyzed by a Battelle-led team. The system will operate in parallel with the utility grid (which provides backup power) to supply either or both of two service/commercial buildings and will feed surplus power into the utility grid. The array consists of fifteen 10-kW carousel-mounted subarrays which are two-axis tracking. The subarrays each consist of 40 passively cooled concentrating modules which incorporate a primary parabolic trough reflector and a secondary compound-elliptic concentrator to achieve a geometric concentration ratio of approx. 26. The power conditioning subsystem is microprocessor controlled, with maximum-power-point tracking and automatic control capabilities. The system performance analysis indicates that the system will supply approximately 147,000 kWh/year to the primary load and an additional 55,000 kWh/year to the utility grid, in the single-load operational mode. The system design and the daily and seasonal match of system output with the load are described in detail. Plans are also discussed for installation and for operational evaluations of performance, economics, and institutional issues.

  4. Utilization of agro-industrial wastes in citric acid fermentation with different techniques%农产品加工副产物在柠檬酸不同发酵类型中的应用

    Institute of Scientific and Technical Information of China (English)

    刘璇; 钱莹; 段钢

    2012-01-01

    柠檬酸作为大宗发酵有机酸之一,被大量应用于食品、制药、洗涤等行业.农产品加工副产物作为原料应用于柠檬酸发酵由于具有成本低,可再生和环境友好等特点,受到了极大的关注.该文总结了近20年关于廉价废弃原料在柠檬酸不同发酵形式中应用的报道,为新型原料的开发提供了一定的理论基础.%As an important bulk chemical, citric acid is widely used in food, pharmaceutical and cleaning industries. The utilization of inexpensive agro-industrial wastes and their by-products is receiving more attention due to its low cost, renewability and environmental benefits. This paper reviewed the studies in recent 20 years, which used novel and economical substrates in citric acid fermentation with different techniques. This provides a new perspective to develop new processes for citric acid production.

  5. Final Report

    DEFF Research Database (Denmark)

    Heiselberg, Per; Brohus, Henrik; Nielsen, Peter V.

    This final report for the Hybrid Ventilation Centre at Aalborg University describes the activities and research achievement in the project period from August 2001 to August 2006. The report summarises the work performed and the results achieved with reference to articles and reports published...

  6. Geochemical Rate/RNA Integration Study (GRIST): A Pilot Field Experiment for Inter-Calibration of Biogeochemistry and Nucleic Acid Measurements Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Bronk, Deborah

    2007-01-08

    The Geochemical Rate/RNA Integration Study (GRIST) project sought to correlate biogeochemical flux rates with measurements of gene expression and mRNA abundance to demonstrate the application of molecular approaches to estimate the presence and magnitude of a suite of biogeochemical processes. The study was headed by Lee Kerkhoff of Rutgers University. In this component of the GRIST study, we characterized ambient nutrient concentrations and measured uptake rates for dissolved inorganic nitrogen (DIN, ammonium, nitrate and nitrite) and dissolved organic nitrogen (urea and dissolved free amino acids) during two diel studies at the Long-Term Ecosystem Observatory (LEO-15) on the New Jersey continental shelf.

  7. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Normanly, J.

    1999-11-29

    The primary goal was the characterization of tryptophan (Trp)-independent biosynthesis of the auxin indole-3-acetic acid (IAA). Our work and that of others indicates that indole is a precursor to IAA in a Trp-independent pathway and the objectives of this grant have been the isolation of indole-metabolizing genes from Arabidopsis.

  8. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Normanly, J.

    1999-11-29

    The primary goal was the characterization of tryptophan (Trp)-independent biosynthesis of the auxin indole-3-acetic acid (IAA). Our work and that of others indicates that indole is a precursor to IAA in a Trp-independent pathway and the objectives of this grant have been the isolation of indole-metabolizing genes from Arabidopsis.

  9. Process development for utilizing asbestos cement waste in rotary kilns for the cement industry. Final report; Erarbeitung eines Verfahrens zur stofflichen Verwertung von zementgebundenen Asbestprodukten in Drehrohroefen fuer die Zementindustrie. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Schlegel, R.; Kieser, J.; Kraehner, A.

    1999-11-01

    The law for recycling and waste demands the utilization also for waste of asbestos cement (ac). The procedure of thermal utilization of ac in the flame of a rotary cement kiln was developed and patented by the research institute IBU-tec Weimar, Germany. The ac-material has to be pre-pulverized and grinded to a degree of fineness of R{sub 90}<15%. Considerations of safety engineering lead to the idea of common fine grinding of old oil (oo) and ac. This new procedure was searched in FuE-project in 1998/99 (financial support by BMBF). A mash of ac and oo was generated as a utilization product ready for firing which was injected into the flame of the rotary cement kiln. This particles of ac smelt to spherical shaped particles at a temperature above 1500 C. They were utilized by clinker formation. The material and gas stream leaving the kiln does not contain fibres of asbestos. This was demonstrated in a small equipment burning test. The industrial realization concerning cement plant Ruedersdorf, near Berlin, was searched, technologically described and safety engineeringly and financially assessed by a project study. Process-technical and financial advantages were seen for the dry fine grinding. The wet fine grinding with old oil could be used in cement plants using old oil as fuel. (orig.) [German] Das Kreislaufwirtschafts- und Abfallgesetz (1994) fordert u.a. die stoffliche Verwertung auch fuer Asbestzementabfaelle (AZ). Das vom Institut fuer Baustoff- und Umweltschutz-Technologie Weimar 1995 entwickelte und patentierte Verfahren zur thermischen Verwertung von AZ in der Flamme eines Zementdrehrohrofens erfuellt diese Forderung. Das AZ-Material muss vorzerkleinert und bis zur Rohmehlfeinheit (R{sub 90}<15%) feingemahlen werden. Sicherheitstechnische Ueberlegungen fuehrten zu der Idee, die Feinmahlung zusammen mit Altoel (AOe) zu erproben. Diese Verfahrensvariante wurde im Rahmen eines FuE-Projektes 1998/99 untersucht (finanzielle Foerderung durch das BMBF). Als

  10. Neutralization of acid mine drainage using the final product from CO{sub 2} emissions capture with alkaline paper mill waste

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Lopez, Rafael, E-mail: rafael.perez@dgeo.uhu.es [Institute of Environmental Assessment and Water Research, IDAEA - CSIC, Jordi Girona 18, 08034 Barcelona (Spain); Department of Geology, University of Huelva, Campus ' El Carmen' , 21071, Huelva (Spain); Castillo, Julio; Quispe, Dino; Nieto, Jose Miguel [Department of Geology, University of Huelva, Campus ' El Carmen' , 21071, Huelva (Spain)

    2010-05-15

    In this study, experiments were conducted to investigate the applicability of low-cost alkaline paper mill wastes as acidity neutralizing agents for treatment of acid mine drainage (AMD). Paper wastes include a calcium mud by-product from kraft pulping, and a calcite powder from a previous study focused on sequestering CO{sub 2} by carbonation of calcium mud. The neutralization process consisted of increase of pH by alkaline additive dissolution, decrease of metals solubility and precipitation of gypsum and poorly crystallized Fe-Al oxy-hydroxides/oxy-hydroxysulphates, which acted as a sink for trace elements to that extent that solutions reached the pre-potability requirements of water for human consumption. This improvement was supported by geochemical modelling of solutions using PHREEQC software, and observations by scanning electron microscope and X-ray diffraction of reaction products. According to PHREEQC simulations, the annual amount of alkaline additive is able to treat AMD (pH 3.63, sulphate 3800 mg L{sup -1}, iron 348 mg L{sup -1}) with an average discharge of about 114 and 40 L s{sup -1} for calcium mud and calcite powder, respectively. Likewise, given the high potential of calcium mud to sequester CO{sub 2} and of resulting calcite powder to neutralize AMD, paper wastes could be a promising solution for facing this double environmental problem.

  11. Biodegradation and reversible inhibitory impact of sulfamethoxazole on the utilization of volatile fatty acids during anaerobic treatment of pharmaceutical industry wastewater

    Energy Technology Data Exchange (ETDEWEB)

    Cetecioglu, Zeynep, E-mail: cetecioglu@itu.edu.tr [Istanbul Technical University, Environmental Engineering Department, 34469 Maslak, Istanbul (Turkey); Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona (Spain); Ince, Bahar [Bogazici University, Institute of Environmental Sciences, Rumelihisarustu - Bebek, 34342 Istanbul (Turkey); Gros, Meritxell; Rodriguez-Mozaz, Sara; Barceló, Damia [Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003 Girona (Spain); Ince, Orhan; Orhon, Derin [Istanbul Technical University, Environmental Engineering Department, 34469 Maslak, Istanbul (Turkey)

    2015-12-01

    This study evaluated the chronic impact and biodegradability of sulfamethoxazole under anaerobic conditions. For this purpose, a lab-scale anaerobic sequencing batch reactor was operated in a sequence of different phases with gradually increasing sulfamethoxazole doses of 1 to 45 mg/L. Conventional parameters, such as COD, VFA, and methane generation, were monitored with corresponding antimicrobial concentrations in the reactor and the methanogenic activity of the sludge. The results revealed that anaerobic treatment was suitable for pharmaceutical industry wastewater with concentrations of up to 40 mg/L of sulfamethoxazole. Higher levels exerted toxic effects on the microbial community under anaerobic conditions, causing the inhibition of substrate/COD utilization and biogas generation and leading to a total collapse of the reactor. The adverse long-term impact was quite variable for fermentative bacteria and methanogenic achaea fractions of the microbial community based on changes inflicted on the composition of the residual organic substrate and mRNA expression of the key enzymes. - Highlights: • Chronic impact of sulfamethoxazole was lethal at 45 mg/L on the microbial community. • Sulfamethoxazole was highly biodegradable under anaerobic conditions. • While the COD removal stopped, the sorption of sulfamethoxazole into the sludge increased. • Sulfamethoxazole has a reversible inhibitory effect on acetoclastic methanogens.

  12. Biodegradation and reversible inhibitory impact of sulfamethoxazole on the utilization of volatile fatty acids during anaerobic treatment of pharmaceutical industry wastewater.

    Science.gov (United States)

    Cetecioglu, Zeynep; Ince, Bahar; Gros, Meritxell; Rodriguez-Mozaz, Sara; Barceló, Damia; Ince, Orhan; Orhon, Derin

    2015-12-01

    This study evaluated the chronic impact and biodegradability of sulfamethoxazole under anaerobic conditions. For this purpose, a lab-scale anaerobic sequencing batch reactor was operated in a sequence of different phases with gradually increasing sulfamethoxazole doses of 1 to 45 mg/L. Conventional parameters, such as COD, VFA, and methane generation, were monitored with corresponding antimicrobial concentrations in the reactor and the methanogenic activity of the sludge. The results revealed that anaerobic treatment was suitable for pharmaceutical industry wastewater with concentrations of up to 40 mg/L of sulfamethoxazole. Higher levels exerted toxic effects on the microbial community under anaerobic conditions, causing the inhibition of substrate/COD utilization and biogas generation and leading to a total collapse of the reactor. The adverse long-term impact was quite variable for fermentative bacteria and methanogenic achaea fractions of the microbial community based on changes inflicted on the composition of the residual organic substrate and mRNA expression of the key enzymes.

  13. Effects of humic acid nitrogen fertilization on corn yield, nitrogen utilization and nitrogen loss%腐植酸氮肥对玉米产量、氮肥利用及氮肥损失的影响

    Institute of Scientific and Technical Information of China (English)

    庄振东; 李絮花

    2016-01-01

    Objective]In order to promote application of the new type fertilizers, increase crop yield and reduce environmental pollution, the research explored effects of humic acid nitrogen fertilizer on corn yield, nitrogen absorption and utilization, and nitrogen distribution and loss in soil.[Methods]The nitrogen isotope tracer technique was used to study the effects of humic acid nitrogen on the nitrogen utilization. Experimental plot soil was circled by specific device. The tested N fertilization treatments were: 1) CK1 (N 0 kg/hm2), 2) CK2 (urea N 225 kg/hm2), 3) HA1 (activated humic acid fertilizer, N 225 kg/hm2), and 4) HA2 (ordinary humic acid nitrogen N 225 kg/hm2). Soil samples in different layers of soil (0–20 cm, 20–40 cm and 40–60 cm) were collected by using earth drilling before planting corn, before fertilization and after the harvest. Ammonia volatilization nitrogen was measured by the static body mothed, and N2O was measured by static body collecting, vacuum flask cold storage and gas chromatograph determining. After the harvest, vegetative organs and grains were separated. After drying, corn production and vegetative organs’ weights were calculated. The vegetative organs and grain were mechanically ground by ball milling ready for measuring.[Results]In grains, the nitrogen amounts from the fertilizers occupied 34.6%–36.2%, and in vegetative organs they occupied 14.6%–17.4%. The utilization rates of common urea and the activated humic acid fertilizer and conventional mixing humic acid fertilizers were 25.1%, 30.9% and 28.5%, respectively, and the loss rates of the nitrogen fertilizers were 38.1%, 19.8% and 27.2%, respectively. The results of application of the activated humic acid fertilizer and conventional mixing humic acid fertilizers compared with common urea were: 1) Maize yields were increased significantly; 2)The total N absorption amounts were increased by 25.8 kg/hm2and 16.3 kg/hm2, respectively;3) The nitrogen utilization rates were

  14. Mitigating Interconnection Challenges of the High Penetration Utility-Interconnected Photovoltaic (PV) in the Electrical Distribution Systems: Cooperative Research and Development Final Report, CRADA Number CRD-14-563

    Energy Technology Data Exchange (ETDEWEB)

    Chakraborty, Sudipta [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-11-01

    Various interconnection challenges exist when connecting distributed PV into the electrical distribution grid in terms of safety, reliability, and stability of the electric power systems. Some of the urgent areas for research, as identified by inverter manufacturers, installers and utilities, are potential for transient overvoltage from PV inverters, multi-inverter anti-islanding, impact of smart inverters on volt-VAR support, impact of bidirectional power flow, and potential for distributed generation curtailment solutions to mitigate grid stability challenges. Under this project, NREL worked with SolarCity to address these challenges through research, testing and analysis at the Energy System Integration Facility (ESIF). Inverters from different manufacturers were tested at ESIF and NREL's unique power hardware-in-the-loop (PHIL) capability was utilized to evaluate various system-level impacts. Through the modeling, simulation, and testing, this project eliminated critical barriers on high PV penetration and directly supported the Department of Energy's SunShot goal of increasing the solar PV on the electrical grid.

  15. Nonclinical safety assessment of recombinant human acid sphingomyelinase (rhASM) for the treatment of acid sphingomyelinase deficiency:the utility of animal models of disease in the toxicological evaluation of potential therapeutics.

    Science.gov (United States)

    Murray, James M; Thompson, Anne Marie; Vitsky, Allison; Hawes, Michael; Chuang, Wei-Lien; Pacheco, Joshua; Wilson, Stephen; McPherson, John M; Thurberg, Beth L; Karey, Kenneth P; Andrews, Laura

    2015-02-01

    Recombinant human acid sphingomyelinase (rhASM) is being developed as an enzyme replacement therapy for patients with acid sphingomyelinase deficiency (Niemann-Pick disease types A and B), which causes sphingomyelin to accumulate in lysosomes. In the acid sphingomyelinase knock-out (ASMKO) mouse, intravenously administered rhASM reduced tissue sphingomyelin levels in a dose-dependent manner. When rhASM was administered to normal rats, mice, and dogs, no toxicity was observed up to a dose of 30mg/kg. However, high doses of rhASM≥10mg/kg administered to ASMKO mice resulted in unexpected toxicity characterized by cardiovascular shock, hepatic inflammation, adrenal hemorrhage, elevations in ceramide and cytokines (especially IL-6, G-CSF, and keratinocyte chemoattractant [KC]), and death. The toxicity could be completely prevented by the administration of several low doses (3mg/kg) of rhASM prior to single or repeated high doses (≥20mg/kg). These results suggest that the observed toxicity involves the rapid breakdown of large amounts of sphingomyelin into ceramide and/or other toxic downstream metabolites, which are known signaling molecules with cardiovascular and pro-inflammatory effects. Our results suggest that the nonclinical safety assessment of novel therapeutics should include the use of specific animal models of disease whenever feasible.

  16. Neoglycopeptide synthesis by suzuki-miyaura couplings between glycosyl aryl boronic acids and iodopeptides.

    Science.gov (United States)

    Chen, Guohua; Gu, Xiangying; Chen, Lin; Wang, Xin; Chen, Yue-Lei; Shen, Jingkang; Zeng, Wenbin

    2014-01-01

    Suzuki-Miyaura coupling reaction was applied in the syntheses of neoglycopeptides. This work utilizes new type of glycosyl aryl boronic acid and readily accessible iodo amino acids/iodopeptides. Both carbohydrate and peptide moieties are unprotected and the final product could be isolated directly. The neoglyco amino acid and neoglycopeptide products feature an O-glycosyl biaryl linker between the carbohydrate and peptide moieties.

  17. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Stinis, Panos [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-08-07

    This is the final report for the work conducted at the University of Minnesota (during the period 12/01/12-09/18/14) by PI Panos Stinis as part of the "Collaboratory on Mathematics for Mesoscopic Modeling of Materials" (CM4). CM4 is a multi-institution DOE-funded project whose aim is to conduct basic and applied research in the emerging field of mesoscopic modeling of materials.

  18. Utilization of carrageenan, citric acid and cinnamon oil as an edible coating of chicken fillets to prolong its shelf life under refrigeration conditions

    Directory of Open Access Journals (Sweden)

    Anshul Kumar Khare

    2016-02-01

    Full Text Available Aim: The present study was conducted to determine efficacy of edible coating of carrageenan and cinnamon oil to enhance the shelf life of chicken meat stored under refrigeration conditions. Materials and Methods: Chicken breast was coated with carrageenan and cinnamon oil by three methods of application viz., spraying brushing and dipping. The coated meat was evaluated for drip loss, pH, thiobarbituric acid number (TBA, tyrosine value (TVextract release volume (ERV, Warner-Bratzler shear force value (WBSFV, instrumental color, microbiological, and sensory qualities as per standard procedures. Results: There was a significant difference observed for physicochemical parameters (pH, TBA, TV, ERV, drip loss and WBSFV and microbiological analysis between storage periods in all the samples and between the control and treatments throughout the storage period but samples did not differed significantly for hunter color scores. However, there was no significant difference among three methods of application throughout the storage period though dipping had a lower rate of increase. A progressive decline in mean sensory scores was recorded along with the increase in storage time. Conclusion: The carrageenan and cinnamon edible coating was found to be a good alternative to enhance the shelf life of chicken meat under refrigeration conditions. It was also observed from study that dipping method of the application had comparatively higher shelf life than other methods of application.

  19. Production of monoclonal antibody for okadaic acid and its utilization in an ultrasensitive enzyme-linked immunosorbent assay and one-step immunochromatographic strip.

    Science.gov (United States)

    Liu, Biing-Hui; Hung, Chun-Tse; Lu, Chuan-Chen; Chou, Hong-Non; Yu, Feng-Yih

    2014-02-12

    Okadaic acid (OA) is a common marine biotoxin that accumulates in bivalves and causes diarrhetic shellfish poisoning (DSP). This study generated a monoclonal antibody (mAb) specific to OA from a hybridoma cell line, 6B1A3, which was obtained by fusion of myeloma cells (P3/NS1/1-AG4-1) with spleen cells isolated from a BALB/c mouse immunized with OA-γ-globulin. The 6B1A3 mAb belongs to the immunoglobulin G1 (κ chain) isotype. Both competitive direct and indirect enzyme-linked immunosorbent assays (ELISAs) were established for characterization of the antibody. The concentrations causing 50% inhibition of binding of OA-horseradish peroxidase to the antibody by OA were calculated to be 0.077 ng/mL in the cdELISA. A rapid and sensitive mAb-based gold nanoparticle immunochromatographic strip was also established. This proposed strip has a detection limit of 5 ng/mL for OA and can be finished in 10 min. Extensive analyses of 20 seafood samples with ELISA revealed that 10 were slightly contaminated with OA, with a mean concentration of 0.892 ng/g. Analysis of OA in shellfish samples showed that data acquired by the immunochromatographic strip agreed well with those acquired by the ELISA. The mAb-based ELISA and immunochromatographic strip assay developed in this study have adequate sensitivity and accuracy for rapid screening of OA in shellfish samples.

  20. Improved Properties of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate Produced by Comamonas sp. EB172 Utilizing Volatile Fatty Acids by Regulating the Nitrogen Source

    Directory of Open Access Journals (Sweden)

    Mohd Rafein Zakaria

    2013-01-01

    Full Text Available This study presents the effect of carbon to nitrogen ratio (C/N (mol/mol on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (Mn of P(3HB-co-3HV copolymer reached the highest at 838 × 103 Da with polydispersity index (PDI value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4. Tensile strength and Young’s modulus of the copolymer containing 6–8 mol% 3HV were in the ranges of 13–14.4 MPa and 0.26–0.34 GPa, respectively, comparable to those of polyethylene (PE. Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials.

  1. Utility of the SeHCAT test in the investigation of different types of bile acid malabsorption; SeHCAT-scanning ved galdesyremalabsorption

    Energy Technology Data Exchange (ETDEWEB)

    Hornshoej Thomsen, Lars; Arveschough, Anne Kirstine; Gustenhoff, Peter; Qvist, Peter

    1998-09-01

    Chronic diarrhoea caused by bile acid malabsorption (BAM) is usually divided into three groups. Type 1 is associated with ileal disease or ileal resection; type 2 is idiopathic, and type 3 is BAM associated with certain predisposing conditions. We evaluated the applicability of the SeHCAT test as a routine investigation of different types of suspected BAM. Detailed information about 298 patients were obtained from retrospective review of patient records. All 68 patients with ileal resections had abnormal SeHCAT retention (median 0.6%; range 0-13%). Of 42 patients with non-resected Crohn`s disease or radiation injury, BAM was found in 28 cases. A diagnosis of BAM type 2 was established in 33 of 150 patients with unexplained chronic diarrhoea. For patients tested for possible BAM type 3, the SeHCAT values were significantly lower compared to type 2 patients. For BAM type 1, the SeHCAT test is only recommended in non-resected patients. Idiopathic BAM seems to be more common than recognized. The presence of certain predisposing conditions might strenghten the indication for SeHCAT testing. (au) 20 refs.

  2. The utilization of a commercial soil nucleic acid extraction kit and PCR for the detection of Clostridium tetanus and Clostridium chauvoei on farms after flooding in Taiwan.

    Science.gov (United States)

    Huang, Shr-Wei; Chan, Jacky Peng-Wen; Shia, Wei-Yau; Shyu, Chin-Lin; Tung, Kwon-Chung; Wang, Chi-Young

    2013-05-02

    Clostridial diseases are zoonoses and are classified as soil-borne diseases. Clostridium chauvoei and Clostridium tetani cause blackleg disease and tetanus, respectively. Since bacteria and spores are re-distributed by floods and then, subsequently, contaminate soils, pastures and water; the case numbers associated with clostridial diseases usually increase after floods. Because Taiwan is often affected by flood damage during the typhoon season, possible threats from these diseases are present. Thus, this study's aim is to apply a combination of a commercial nucleic acid extraction kit and PCR to assess the prevalence of Clostridia spp. in soil and to compare the positivity rates for farms before and after floods. The minimum amounts of Clostridium tetanus and Clostridium chauvoei that could be extracted from soils and detected by PCR were 10 and 50 colony forming units (cfu), respectively. In total, 76 samples were collected from the central and southern regions of Taiwan, which are the areas that are most frequently damaged by typhoons. Noteworthy, the positive rates for Clostridium tetanus and Clostridium chauvoei in Pingtung county after the severe floods caused by a typhoon increased significantly from 13.73 and 7.84% to 53.85 and 50.00%, respectively. This study for the first time provides the evidence from surveillance data that there are changes in the environmental distribution of Clostridium spp. after floods. This study indicates that screening for soil-related zoonotic pathogens is a potential strategy that may help to control these diseases.

  3. Regeneration of barium carbonate from barium sulphide in a pilot-scale bubbling column reactor and utilization for acid mine drainage.

    Science.gov (United States)

    Mulopo, J; Zvimba, J N; Swanepoel, H; Bologo, L T; Maree, J

    2012-01-01

    Batch regeneration of barium carbonate (BaCO(3)) from barium sulphide (BaS) slurries by passing CO(2) gas into a pilot-scale bubbling column reactor under ambient conditions was used to assess the technical feasibility of BaCO(3) recovery in the Alkali Barium Calcium (ABC) desalination process and its use for sulphate removal from high sulphate Acid Mine Drainage (AMD). The effect of key process parameters, such as BaS slurry concentration and CO(2) flow rate on the carbonation, as well as the extent of sulphate removal from AMD using the recovered BaCO(3) were investigated. It was observed that the carbonation reaction rate for BaCO(3) regeneration in a bubbling column reactor significantly increased with increase in carbon dioxide (CO(2)) flow rate whereas the BaS slurry content within the range 5-10% slurry content did not significantly affect the carbonation rate. The CO(2) flow rate also had an impact on the BaCO(3) morphology. The BaCO(3) recovered from the pilot-scale bubbling column reactor demonstrated effective sulphate removal ability during AMD treatment compared with commercial BaCO(3).

  4. Final report on the safety assessment of Hydrogenated Cottonseed Oil, Cottonseed (Gossypium) Oil, Cottonseed Acid, Cottonseed Glyceride, and Hydrogenated Cottonseed Glyceride.

    Science.gov (United States)

    2001-01-01

    Hydrogenated Cottonseed Oil, Cottonseed (Gossypium) Oil, Cottonseed Acid, Cottonseed Glyceride, and Hydrogenated Cottonseed Glyceride are cosmetic ingredients derived from Cottonseed Oil and used as skin-conditioning agents and surfactants. Nonoils known to be toxic that may be found in cottonseed oils include gossypol, aflatoxin, and cyclopropenoid fatty acids (CPFA). Toxic heavy metal and/or polychlorinated biphenyl (PCB) or other pesticide contamination is also possible. Cottonseed Oil was nontoxic in acute oral toxicity studies in rats. In a short-term study, rabbits that had been fed 2% Cottonseed Oil for 7 weeks had significantly lower blood chemistry parameters (compared to wheat bran controls) and significantly more stored hepatic vitamin A (compared to rabbits fed other fats). Cottonseed Oil controls used as vehicles in two parenteral studies produced negative results. Hydrogenated Cottonseed Oil tested in formulation did not produce dermal or ocular irritation in rabbits. An oral-dose reproductive study tested up to 30% Cottonseed Oil (with 1% CPFAs) and reported no adverse effects on sexual maturity and reproductive performance of the F0 generation; changes were noted in the F1 generation but reproductive capacity was not altered. Parenteral-dose reproductive studies reported no adverse effects. Cottonseed Oil was not mutagenic. Cottonseed Oil did not induce aberrant crypt foci when given orally to mice, but in other studies, it increased the incidence of spontaneous mammary tumors in rats and mice. Mice fed 20% Hydrogenated Cottonseed Oil during induction and promotion of photocarcinogenesis had significantly lower tumor incidence compared to mice fed 20% sunflower oil. Hydrogenated Cottonseed Oil in formulation (up to approximately 21%) was neither an irritant nor sensitizer in clinical studies. Limited clinical data indicated that Cottonseed Oil does not contain allergic protein. Based on the available data, it was concluded that these ingredients may

  5. Engineering study of a 20 MW lead--acid battery energy storage demonstration plant. Final report for the period ending October 1976

    Energy Technology Data Exchange (ETDEWEB)

    1976-10-01

    The Research and Engineering Operation of Bechtel Corporation conducted an engineering study of a 20-MW lead--acid battery energy storage demonstration plant. Ten alternative designs were evaluated. Basically, the configurations proposed for the demonstration plants are those of the mature plants which would follow. The designs of the individual plants are based on the cell designs and the means used to house the cells. Initially, proposed cell designs from five manufacturers were considered. To conform with the level of effort allowed for this engineering study, two manufacturers' cells (one open-tank design and one sealed cell design) were selected by ERDA and Bechtel as being representative. These designs formed the basis for the detailed evaluation conducted in this study. The plant and battery configurations evaluated in the study are a large open-tank cell, configured in rows and housed in four buildings; a sealed cell, configured in a single layer of close packed rows in a single building; a sealed cell, configured in a three-tiered arrangement in a single building; and a sealed cell, configured with groups of cells housed in weatherproof modules and placed outdoors. Annual operating costs based on these mature plant costs show lead--acid load-leveling plants are generally not economically competitive with the alternatives when no consideration is given to their other possible benefits to the power system. However, application of credits (e.g., transmission line or spinning reserve credits) can make such plants economically competitive with gas turbine peaking units in specific situations. 46 figures, 25 tables. (RWR)

  6. Review of the recycling utilization of poly-lactic acid waste%聚乳酸废料的回收再利用述评

    Institute of Scientific and Technical Information of China (English)

    郑辉林; 苏海丽; 张辉旋; 林建强; 李亚东

    2013-01-01

    聚乳酸废料的改性再利用的方法有物理改性、化学改性和物理化学改性.目前,国内外企业主要通过PLA增黏和耐热改性等物理化学方法,改善其成型技术难度大及产品耐热不足的问题.随着成本的降低以及性能的提高,PLA的应用范围将越来越广,特别是在医学领域,它具有其他材料无可替代的作用.因此,今后应把PLA废料的再利用扩展到医学领域,尝试用新材料对PLA废料进行改性,在克服原有缺点的基础上开发出新用途的PLA材料.%The methods of poly-lactic acid waste recycling include the physical modification, chemical modification and physi-chemical modification. At present, domestic and foreign enterprises mainly use physi-chemical methods of tackifying and heat resistance to improve the difficult molding technology and the problem of insufficient heat resistance. With the cost reduction and performance improvement, the application scope of PLA will be more and more widely, especially in the medical field, it has other irreplaceable role. Therefore, the PLA waste re-use should be extended to the field of medicine, and try to modify PLA waste with new materials to develop new uses of the PLA material in the base of overcoming the shortcomings.

  7. Utilization of theoretical studies of the imprinting ratio to guide experimental research into the molecular imprinted polymers formed using enrofloxacin and methacrylic acid.

    Science.gov (United States)

    Liu, Junbo; Dai, Zhengqiang; Li, Bo; Tang, Shanshan; Jin, Ruifa

    2014-10-01

    Computational approaches have been suggested as rational and fast methods for optimizing imprinting ratios. The B3LYP/6-31 g(d,p) level was applied to simulate the self-assembled system of molecularly imprinted polymers (MIPs) formed by enrofloxacin (ENRO) and methacrylic acid (MAA). Geometry optimization, the bonding situation, and the binding energies involved were studied to determine the impact of varying the imprinting ratio on the recognition characteristics. These theoretical results showed that the compound with an ENRO:MAA ratio of 1:7 had the lowest binding energy and the most stable structure. MIPs with different imprinting ratios of ENRO to MAA were then prepared in order to study the binding capacities of the polymers experimentally. The experimental and theoretically calculated results for these polymers were found to be consistent with each other. In dynamic adsorption experiments on the MIPs, the adsorption reaction was observed to reach a balanced state after 120 min. Analysis of the Scatchard plot revealed that the dissociation constant (K d) and the apparent maximum binding capacity (Q max) of MIPs with high-affinity sites were 451.67 mg/L and 42.23 mg/g, respectively, whereas the dissociation constant and apparent maximum binding capacity of MIPs with low-affinity sites were 883.39 mg/L and 73.15 mg/g, respectively. The quantity of ENRO adsorbed onto the MIPs was considerably higher than the quantities of ciprofloxacin (CIP) and ofloxacin (OFL) adsorbed, indicating that these MIPs have a much higher specific absorption capacity than the corresponding non-imprinted polymers.

  8. Clinical utility of a nested nucleic acid amplification format in comparison to viral culture for the diagnosis of mucosal herpes simplex infection in a genitourinary medicine setting

    Directory of Open Access Journals (Sweden)

    Wyatt Dorothy E

    2001-08-01

    Full Text Available Abstract Background Nested nucleic acid amplification tests are often thought too sensitive or prone to generatingfalse positive results for routine use. The current study investigated the specificity and clinicalutility of a routine multiplex nested assay for mucosal herpetic infections. Methods Ninety patients, categorised into those clinically diagnosed to (a have and (b not haveherpetic infection, were enrolled. Swabs from oral and ano-genital sites were assayed by thenested assay and culture and the results assessed against clinical evaluation for diagnosingherpetic infections; cell content was also recorded. Results Twenty-six and 64 patients were thought to (a have and (b not have mucosal herpeticinfection. Taking the clinical evaluation as indicating the presence of herpetic infection, thenested polymerase chain reaction and culture had respective sensitivities of 19/26 (73% and12/26 (46% (Χ2 p = 0.02. There was no significant difference in specificities between nPCR62/64 (97% and culture 63/64 (98% (Χ2 p = 1.0. Cell content was important for viraldetection by nPCR (Χ2 p = 0.07 but not culture. Nesting was found necessary for sensitivity anddid not reduce specificity. Assay under-performance appeared related to sub-optimal cellcontent (20% but may have reflected clinical over-diagnosis. The results suggest the need forvalidating specimen cell quality. Conclusions This study questions the value of routine laboratory confirmation of mucosal herpetic infection. The adoption of a more discriminatory usage of laboratory diagnostic facilities for genital herpetic infection, taking account of cell content, and restricting it to those cases where it actually affects patient management, may be warranted.

  9. Integration of thermal and food processing residuals into a system for commercial culture of freshwater shrimp. (power plant waste heat utilization in aquaculture). Volume II. Final report Jul 74--Oct 76

    Energy Technology Data Exchange (ETDEWEB)

    Eble, A.F.

    1977-01-01

    It has been demonstrated that all life-cycle stages of the tropical freshwater prawn, Macrobrachium rosenbergii, can be cultured successfully using waste-heat effluents of the Mercer Generating Station, Trenton, N.J. Further, high-density culture of the prawn is possible and practical. Rainbow trout (Salmo gairdneri) culture has also been successfully demonstrated utilizing the waste-heat discharges of an electric generating station. Efficient systems have been designed for intensive annual two-crop production. Postlarval prawns are grown in indoor heated nurseries in early spring, and placed in outdoor ponds in mid-May at sizes of 5 to 6cm and harvested in late October as 11 to 12cm adults. Experiments culturing the American eel (Anguilla rostrata) in waste-heat discharge waters of the generating system have been successful. Presented in Volume 2 is the research work conducted in this study. (Portions of this document are not fully legible)

  10. Solubilities and transport properties of the stratospheric sulphuric acid aerosol: laboratory investigations using extremely thin sulphuric acid films. Final report; Loesungs- und Transporteigenschaften des stratosphaerischen Schwefelsaeureaerosols: Laboruntersuchungen an extrem duennen Schwefelsaeurefilmen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Schurath, U.; Proksch, V.

    2000-04-17

    We have developed a procedure to measure solubilities, diffusion coefficients and rate constants (oxidation; hydrolysis) of trace gases in sulphuric acid at stratospheric temperatures. The results will lead to a better understanding and quantification of heterogeneous reactions in/on sulphuric acid aerosol particles in stratospheric models. Sulphuric acid (19-83 wt-%) is deposited as a thin film (1-3 {mu}m) on the inner surface of a capillary column (i.d. 530 {mu}m). The trace gas under study is injected into the carrier gas which passes through the column. Solubilities, diffusion coefficients and rate constants for the hydrolysis/oxidation of the trace gases in the sulphuric acid film can be deduced from the retention times, shapes and areas of the trace gas peaks which are detected by a mass spectrometer. (orig.) [German] Es wurde ein Verfahren zur Messung von Loeslichkeiten, Diffusionskoeffizienten und Ratenkonstanten (Oxidation; Hydrolyse) von Spurengasen in Schwefelsaeure bei stratosphaerischen Temperaturen entwickelt. Die Messergebnisse dienen dem besseren Verstaendnis und der Quantifizierung von heterogenen Reaktionen am stratosphaerischen Schwefelsaeure-Aerosol in Stratosphaerenmodellen. Die Schwefelsaeure (19-83 Gew.%) befindet sich als duenner Film (1-3 {mu}m) auf der Innenseite einer Kapillarsaeule (ID=530 {mu}m), die von einem Traegergas und dem zu untersuchenden Spurengas durchstroemt wird. Die Kapillarsaeule wird in einem Kryostaten auf 186-263 K gekuehlt. Aus der Ankunftszeit, Form und Flaeche von Spurengaspeaks an einem Massenspektrometer-Detektor koennen Loeslichkeiten und Diffusionskoeffizienten sowie Ratenkonstanten fuer die Hydrolyse/Oxidation von Spurengasen im Schwefelsaeurefilm bestimmt werden. (orig.)

  11. Aspects of carbon dioxide utilization

    Energy Technology Data Exchange (ETDEWEB)

    Omae, Iwao [Omae Research Laboratories, 335-23 Mizuno, Sayama, Saitama 350-1317 (Japan)

    2006-06-30

    Carbon dioxide reacts with hydrogen, alcohols, acetals, epoxides, amines, carbon-carbon unsaturated compounds, etc. in supercritical carbon dioxide or in other solvents in the presence of metal compounds as catalysts. The products of these reactions are formic acid, formic acid esters, formamides, methanol, dimethyl carbonate, alkylene carbonates, carbamic acid esters, lactones, carboxylic acids, polycarbonate (bisphenol-based engineering polymer), aliphatic polycarbonates, etc. Especially, the productions of formic acid, formic acid methyl ester and dimethylformamide with a ruthenium catalyst; dimethyl carbonate and urethanes with a dialkyltin catalyst; 2-pyrone with a nickel-phosphine catalyst; diphenyl carbonate with a lead phenoxide catalyst; the alternating copolymerization of carbon dioxide and epoxides with a zinc catalyst has attracted attentions as the industrial utilizations of carbon dioxide. The further development of these production processes is expected. (author)

  12. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    R Paul Drake

    2004-01-12

    OAK-B135 This is the final report from the project Hydrodynamics by High-Energy-Density Plasma Flow and Hydrodynamics and Radiation Hydrodynamics with Astrophysical Applications. This project supported a group at the University of Michigan in the invention, design, performance, and analysis of experiments using high-energy-density research facilities. The experiments explored compressible nonlinear hydrodynamics, in particular at decelerating interfaces, and the radiation hydrodynamics of strong shock waves. It has application to supernovae, astrophysical jets, shock-cloud interactions, and radiative shock waves.

  13. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Jarillo-Herrero, Pablo [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2017-02-07

    This is the final report of our research program on electronic transport experiments on Topological Insulator (TI) devices, funded by the DOE Office of Basic Energy Sciences. TIbased electronic devices are attractive as platforms for spintronic applications, and for detection of emergent properties such as Majorana excitations , electron-hole condensates , and the topological magneto-electric effect . Most theoretical proposals envision geometries consisting of a planar TI device integrated with materials of distinctly different physical phases (such as ferromagnets and superconductors). Experimental realization of physics tied to the surface states is a challenge due to the ubiquitous presence of bulk carriers in most TI compounds as well as degradation during device fabrication.

  14. Effect of ethylene diamine tetra-acetic acid, MTAD TM , and HEBP as a final rinse on the microhardness of root dentin

    Directory of Open Access Journals (Sweden)

    Mukura Kulasekaran Dineshkumar

    2012-01-01

    Aims: This study evaluated the effect of 17% EDTA, MTAD, and 18% HEBP solutions on the microhardness of human root canal dentin using the Vickers microhardness test. Materials and Methods: Forty human single-rooted teeth were decoronated at the cementoenamel junction and sectioned longitudinally into buccal and lingual segments. Eighty specimens were divided into four groups (n=20. Group I was treated with distilled water (control, groups II, III, and IV were treated with 1.3% NaOCl as a working solution for 20 minutes followed by 17% EDTA, MTAD, and 18% HEBP respectively. The surface hardness of the root dentin was determined in each specimen with a Vicker′s hardness tester. The values were statistically analyzed using the one-way ANOVA and post hoc Tukey multiple comparison tests. Results: There was a statistical significant difference among all the groups (one-way ANOVA; P<0.001. Among the experimental groups, HEBP showed the highest dentin microhardness (53.74 MPa, P<0.001. Least microhardness was found with MTAD (42.85 MPa, P<0.001. Conclusions: HEBP as a final rinse appears to be a promising irrigation protocol with less impact on the mineral content of root dentin.

  15. Collection of VLE data for acid gas - alkanolamine systems using Fourier transform infrared spectroscopy. Final report, September 29, 1990--September 30, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Bullin, J.A.; Rogers, W.J.

    1996-11-01

    This report describes research from September 29, 1990 through September 30, 1996, involving the development a novel Fourier transform infrared (FTIR) spectroscopic apparatus and method for measuring vapor - liquid equilibrium (VLE) systems of carbon dioxide and hydrogen sulfide with aqueous alkanolamine solutions. The original apparatus was developed and modified as it was used to collect VLE data on acid gas systems. Vapor and liquid calibrations were performed for spectral measurements of hydrogen sulfide and carbon dioxide in the vapor and in solution with aqueous diethanolamine (DEA) and methyldiethanolamine (MDEA). VLE measurements were made of systems of hydrogen sulfide and carbon dioxide in 20 wt % DEA at 50{degrees}C and 40{degrees}C. VLE measurements were made of systems of hydrogen sulfide and carbon dioxide in 50 wt% and 23 wt% MDEA at 40{degrees}C and in 23 wt% MDEA at 50{degrees}C. VLE measurements were made of systems of hydrogen sulfide and carbon dioxide in 35 wt% MDEA + 5 wt% DEA and in 35 wt% MDEA + 10 wt% DEA at 40{degrees}C and 50{degrees}C. Measurements were made of residual amounts of carbon dioxide in each VLE system. The new FTIR spectrometer is now a consistently working and performing apparatus.

  16. 磷肥副产物氟硅酸中氟硅资源综合利用技术综述%Overview of Technology of Comprehensive Utilization of Fluosilicic Acid from Byproducts of Phosphate Fertilizer

    Institute of Scientific and Technical Information of China (English)

    周绿山

    2015-01-01

    The fluosilicic acid is a byproduct from production of phosphate fertilizer, its recovery and utilization technology is developed gradually from producing sodium fluorosilicate only to production of white carbon black and other products.An overview is given of several new processes for production of white carbon black by fluosilicic acid and co-production of hydrogen fluoride, ammonium bifluoride, cryolite, sodium fluoride, and so on. The advantages and disadvantages of each process are summarized.%氟硅酸是磷肥生产过程中的副产物,其回收利用技术由仅能生产氟硅酸钠逐渐发展为可以生产白炭黑等产品。概述了多种由氟硅酸制备白炭黑联产氟化氢、氟化氢铵、冰晶石、氟化钠等新工艺,并简要总结了各工艺的优缺点。

  17. 磷酸储罐沉渣的清理新方法及利用%New cleaning technology for sediment in phosphoric acid storage tank and utilization of sediment

    Institute of Scientific and Technical Information of China (English)

    杨振军; 刘翠丽

    2012-01-01

    The cause of sediment in phosphoric acid storage tank and the existing problems in sediment cleaning such as sediment emission, collapse and pollution etc. are analyzed. The new cleaning technology for sediment in phosphoric acid storage tank and the comprehensive utilization of sediment are introduced, which can liquidize the production raw material with value of more than 6 million RMB Yuan, and save the labor and transportation cost of more than 0.3 million 1RMB Yuan.%分析磷酸储罐沉渣的成因及储罐清理过程中面临的沉渣涌出、塌方、过程污染等问题。介绍清理磷酸沉渣的新方法及利用。利用新方法清理沉渣盘活了价值600多万元的生产原料,节省人工费、运输费用共计30多万元。

  18. Study on the influence of storage life expectancy of the Valve Regulated Lead-Acid - VRLA battery; Estudo sobre a influencia da estocagem na expectativa de vida util da bateria chumbo-acida regulada por valvula - VRLA

    Energy Technology Data Exchange (ETDEWEB)

    Soares, A. Pinhel [FURNAS Centrais Eletricas S.A., Rio de Janeiro, RJ (Brazil)], Email: pinhel@furnas.com.br; Rosolem, Maria de F.N.C.; Santos, G.R. dos; Frare, P.T.; Arioli, V.T.; Beck, R.F. [Telecomunicacoes do CPqD, Campinas, SP (Brazil)], Emails: mfatima@cpqd.com.br, glauco@cpqd.com.br, pfrare@cpqd.com.br, varioli@cpqd.com.br, raul@cpqd.com; Soares, L.A., Email: luiz.las@gmail.com

    2009-07-01

    When valve regulated lead-acid (VRLA) batteries are acquired and are not placed in operation immediately and remain stored in open circuit, they can loose autonomy and life. In these cases the current practice recommends, that the batteries receive quarterly recharges, which is often unfeasible. Given this scenario, Furnas by the CPqD, decided to verify the real impact of stockpiling in the expectancy of VRLAs battery life to establish the veracity of practice adopted or establish new procedures. The influences of time, the temperature of the local storage and application of charges are evaluated. It was also studied the application of techniques for measuring the internal resistance battery (conductance and impedance) for degradation monitoring and identification of the need for application of charges. As final products, it was developed novel diagnostic techniques that allow more accurate monitoring of the storage process.

  19. Qualidade final de melão osmoticamente desidratado em soluções de sacarose com adição de ácidos Quality of osmotically dehydrated melon in sucrose solutions with addition of acids

    Directory of Open Access Journals (Sweden)

    Eliana Janet Sanjinez Argandoña

    2002-12-01

    Full Text Available A desidratação osmótica associada à adição de ácidos fracos representa uma alternativa de processamento brando, resultando em um produto com características sensoriais praticamente inalteradas e apropriado para o consumo imediato. O objetivo deste estudo foi avaliar a influência dos ácidos cítrico e lático na obtenção de melão osmoticamente desidratado e na qualidade final do produto. Pedaços de melão (Cucumis melo inodorus, cultivar: Gold Mine, de 40x30x15 mm, foram imersos em três tipos de soluções (sacarose + ácido cítrico, sacarose + ácido lático e sacarose com diferentes concentrações de sacarose (50 a 70ºBrix. A desidratação osmótica foi realizada em temperatura controlada (30 a 50ºC por até três horas. A adição de ácidos não influenciou significativamente na variação da cromaticidade. No entanto, a concentração da solução desidratante e a temperatura tiveram efeitos significativos no aumento da luminosidade do produto. A tensão na ruptura foi menor nas amostras processadas em relação à fruta fresca, porém a deformação na ruptura foi significativamente mais alta nas amostras tratadas com ácido lático a 50ºC, fornecendo produtos mais viscoelásticos, porém mais firmes.The combination of osmotic dehydration and weak acids addition is a mild process that results in a final product with organoleptic characteristics very similar to fresh and "ready to eat" fruit, appropriate for immediate consumption. The purpose of this work was to evaluate the influence of citric and lactic acids on the production of osmotically dehydrated melon and on its final quality. Melon (Cucumis melo inodorus, cultivar Gold Mine pieces of 40x30x15 mm were immersed in three types of dehydrating solutions (sucrose + citric acid, sucrose + lactic acid and control of different concentrations (50 to 70ºBrix. Osmotic dehydration was carried out for up to three hours under controlled temperature (30 to 50ºC. The addition

  20. Reactive dye extraction utilizing regenerated bleaching earth

    Directory of Open Access Journals (Sweden)

    M. Shahi

    2017-09-01

    Full Text Available Bentonite bleaching earth is utilized for purifying used motor oil through a recovery process in order to improve the quality and stability of the final product. Indeed, spent bleaching earth is generated due to adsorbing oil impurities. Polluted spent bleaching earth contains 20-40% (w/w oil and is flammable. Its disposal without pre-treatment leads to loss of oil along with environmental impacts. Accordingly, similar studies have been conducted since 1979 until now. This research was a laboratory study on reactive dye adsorption. Cleaning bleaching clay, thermal remediation and acid washing activation methods were utilized. Response surface methodology was used to design the experiments and determine the optimal parameters in order to run the dye adsorption process. The main experimental parameters have been concluded as temperature (200-800 °C, acid solution concentration (0.1-3 M, dye solution concentration (1-35 ppm, and ratio of activated earth to dye solution (0.1-2 %, w/w. Results revealed that dye adsorption process along with oil removal at a temperature of 650 °C, acid solution concentration of 0.83 M, dye solution concentration of 11.75 ppm and ratio of activated earth to dye solution of 1.52 % (w/w results in an adsorption efficiency of 68.57%. This removal efficiency is a bit higher than activated virgin bleaching earth and much higher than virgin bleaching earth, which has adsorption capacities of 66.75% and 51.56%, respectively. Considering this recycling process, the purified material is quite acceptable technically, environmentally and economically.

  1. Greenhouse gas reduction by recovery and utilization of landfill methane and CO{sub 2} technical and market feasibility study, Boului Landfill, Bucharest, Romania. Final report, September 30, 1997--September 19, 1998

    Energy Technology Data Exchange (ETDEWEB)

    Cook, W.J.; Brown, W.R.; Siwajek, L. [Acrion Technologies, Inc., Cleveland, OH (United States); Sanders, W.I. [Power Management Corp., Bellevue, WA (United States); Botgros, I. [Petrodesign, SA, Bucharest (Romania)

    1998-09-01

    The project is a landfill gas to energy project rated at about 4 megawatts (electric) at startup, increasing to 8 megawatts over time. The project site is Boului Landfill, near Bucharest, Romania. The project improves regional air quality, reduces emission of greenhouse gases, controls and utilizes landfill methane, and supplies electric power to the local grid. The technical and economic feasibility of pre-treating Boului landfill gas with Acrion`s new landfill gas cleanup technology prior to combustion for power production us attractive. Acrion`s gas treatment provides several benefits to the currently structured electric generation project: (1) increase energy density of landfill gas from about 500 Btu/ft{sup 3} to about 750 Btu/ft{sup 3}; (2) remove contaminants from landfill gas to prolong engine life and reduce maintenance;; (3) recover carbon dioxide from landfill gas for Romanian markets; and (4) reduce emission of greenhouse gases methane and carbon dioxide. Greenhouse gas emissions reduction attributable to successful implementation of the landfill gas to electric project, with commercial liquid CO{sub 2} recovery, is estimated to be 53 million metric tons of CO{sub 2} equivalent of its 15 year life.

  2. Development of a two-phase, two-component jet pump refrigerator for utilization of low-temperature solar heat. Final report; Entwicklung einer Zweiphasen-/Zweikomponenten-Strahlpumpenkaelteanlage zur Nutzung solarer Niedertemperaturwaerme. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Mostofizadeh, C.; Bohne, D.

    2001-08-01

    A solar refrigerator for air conditioning and cooling was developed. The key component is a two-phase, two-component jet pump with ammonia and water as working fluid. Both the pump flow and the suction flow can be two-phase flows. This way, the advantages of both the absorption and the compression principle will be utilized, and a compact size will be achieved. Upon termination of the calculations, the function of the pump will be investigated in a 0 W pilot plant. For this, various geometries were calculated and tested for their potential efficiencies. A prototype will be constructed on the basis of the results. [German] Das Ziel des Vorhabens besteht in der Entwicklung einer solarbetriebenen Kaelteanlage fuer Klimatisierungs- und Kuehlungszwecke. Die Hauptkomponente der Kaelteanlage ist eine Zweiphasen-/Zweikomponenten-Strahlpumpe, die mit dem Arbeitsgemisch Ammoniak/Wasser betrieben wird. Sowohl der Treib- als auch der Saugstrom koennen zweiphasig sein. Dadurch sollen einerseits die Vorteile des Absorptions- und des Kompressionsprinzips miteinander verknuepft und andererseits ein kompakter Aufbau erreicht werden. Nach Abschluss der thermodynamischen und kinetischen Berechnungen soll die Funktion der Zweiphasen-/Zweikomponenten-Strahlpumpe mit Hilfe einer Pilotanlage mit ca. 20 kW Kaelteleistung untersucht werden. Dazu werden nach Vorausberechnungen verschiedene Geometrien in Bezug auf erzielbare Wirkungsgrade getestet. Die Ergebnisse bilden die Basis fuer den Bau eines Prototyps. (orig.)

  3. 叶酸利用能力分子医学检测在母胎医学领域的应用%Application of molecularmedical examination of folic acid utilization ability in maternal-fetal medicine

    Institute of Scientific and Technical Information of China (English)

    李秀娟; 徐敏; 徐梅; 杨燕; 卢伟; 于雪梅; 姜晶

    2014-01-01

    目的:探讨分子医学检测叶酸利用能力在母胎医学领域的研究与应用。方法通过检测MTHFR基因C677T、A1298 C及MTRR基因A66 G相关多态性位点,分析其遗传特征及分布特点,从而对叶酸利用能力遗传风险进行分级。结果青岛地区围产期妇女发现高度叶酸利用能力遗传风险的比例为23.17%,显著高于全国参考数据的17.70%(χ2=7.136,P<0.05);中度和低度叶酸利用能力遗传风险的比例分别为33.07%和18.19%,全国参考数据为35.18%和12.08%,无显著性差异(χ2值分别为7.452、2.347,均P>0.05),未发现遗传风险的比例为25.57%,显著低于全国参考数据的33.04%(χ2=8.134,P<0.05)。实验组与对照组的叶酸利用能力总体构成不同,存在统计学差异(χ2=15.67,P<0.01),高度风险组与未发现风险组的相对危险度( RR)为2.61。结论青岛地区围产期妇女叶酸代谢和利用障碍与不良孕产妇有着重要的关系,基于Taqman-MGB探针检测的孕期叶酸补充指导和监测将是进一步降低新生儿出生缺陷的重要方法。%[ Abstact] Objective To explore the application of molecular medical examination of folic acid utilization ability in maternal-fetal medicine.Methods By detecting MTHFR gene C677T, A1298C and MTRR gene A66G related polymorphisms, their genetic characteristics and distribution were analyzed, and thus to classify the genetic risk of folic acid utilization ability.Results In Qingdao the high genetic risk of folic acid utilization ability in perinatal women was 23.17%, which was significantly higher than the national reference data (17.70%) (χ2 =7.136, P0.05).Genetic risk was not found in 25.57%perinatal women, which was obviously lower than the national reference data (33.04%) (χ2 =8.134,P<0.05).The overall composition of folic acid utilization ability in the experimental group and

  4. Amino acids analysis during lactic acid fermentation by single strain ...

    African Journals Online (AJOL)

    SAM

    2014-07-09

    Jul 9, 2014 ... of the three LAB strains to utilize amino acids for growth and lactic acid production were employed to ... Lactic acid bacteria (LAB), which are used for the ..... and characterization of potential probiotic lactobacilli from pig feces.

  5. Production development and utilization of Zimmer Station wet FGD by-products. Final report. Volume 6, Field study conducted in fulfillment of Phase 3 titled. Use of FGD by-product gypsum enriched with magnesium hydroxide as a soil amendment

    Energy Technology Data Exchange (ETDEWEB)

    Bigham, J. M. [Ohio State Univ., Wooster, OH (United States). Ohio Agricultural Research Development Center; Soto, U. I. [Ohio State Univ., Wooster, OH (United States). Ohio Agricultural Research Development Center; Stehouwer, R. C. [Ohio State Univ., Wooster, OH (United States). Ohio Agricultural Research Development Center; Yibirin, H. [Ohio State Univ., Wooster, OH (United States). Ohio Agricultural Research Development Center

    1999-04-30

    A variety of flue gas desulfurization (FGD) technologies have been developed to meet environmental restrictions imposed by the federal Clean Air Act and its amendments. These technologies include wet scrubber systems that dramatically reduce sulfur dioxide (SO2) emissions. Although such systems are effective, they also produce large volumes of sludge that must be dewatered, stabilized, and disposed of in landfills. Disposal is an expensive and environmentally questionable process for which suitable alternatives are needed. Wet scrubbing of flue gases with magnesium (Mg)-enhanced lime has the potential to become a leading FGD technology. When combined with aforced oxidation system, the wet sludges resulting from this process can be modified and refined to produce gypsum (CaS04∙2H2O) and magnesium hydroxide [Mg(OH)2] of sufficient purity for beneficial re-use in the construction (wallboard) and pharmaceutical industries. The pilot plant at the CINERGY Zimmer Station near Cincinnati can also produce gypsum by-products formulated to contain varying amounts of Mg(OH)2- Such materials may have value to the agriculture, forestry, and lawn-care industries as soil "conditioners", liming agents, and nutritional supplements capable of supplying calcium (Ca), Mg, and sulfur (S) for plant growth. This report describes three field studies designed to evaluate by-product gypsum and Mg-gypsum from the Zimmer Station power plant as amendments for improving the quality of mine spoils and agricultural soils that were unproductive because of phytotoxic levels of dissolved aluminum (Al) and low pH. The technical literature suggests that gypsum may be more effective than agricultural limestone for ameliorating Al toxicity below the immediate zone of application. Such considerations are important for deep-rooted plant species that attempt to utilize water and nutrients occurring at depth in the spoil/soil.

  6. Preliminary analysis of the state of the art of robotics and precision engineering and evaluation of potential for improved energy utilization in the pulp, paper, and related energy-consuming processes. Final report

    Energy Technology Data Exchange (ETDEWEB)

    None

    1982-01-01

    This study was undertaken to conduct a preliminary analysis of the state of the art of two technologies, robotics and precision engineering, and to evaluate their potential for improved energy utilization in the pulp, paper, and related energy consuming processes. Activity in the robotics field is growing rapidly, most activity being related to the development of smart robots rather than to systems. There is a broad base of support, both in industry and the universities, for upgrading robot machine capabilities. A large part of that support is associated with visualization and tactile sensors which facilitate assembly, placement, inspection, and tracking. Progress in this area is relatively rapid and development times are short for specifically engineered applications. The critical path in the development of robotic systems lies in the generation of reliable sensor signals. Robotic systems require a broad spectrum of sensors from which hierarchical logic systems can draw decision making information. This requirement resulted in the establishment of a program at the National Bureau of Standards which is attempting to develop a spectrum of sensor capabilities. Such sensors are applicable to robotic system automatic process control in a variety of energy-intensive industries. Precision engineering is defined as the generation or manufacture of components wherein geometry, dimension, and surface finish are controlled to within several hundred Angstroms in single point turning operations. Investigation into the state of the art of precision engineering in the United States finds that this capability exists in several national laboratories and is intended to be used exclusively for the development of weapons. There is an attempt at the present time by Lawrence Livermore Laboratory to expand its capability into industry. Several corporations are now beginning to develop equipment to support the precision engineering field.

  7. Cost Effective Bioethanol via Acid Pretreatment of Corn Stover, Saccharification, and Conversion via a Novel Fermentation Organism: Cooperative Research and Development Final Report, CRADA Number: CRD-12-485

    Energy Technology Data Exchange (ETDEWEB)

    Dowe, N.

    2014-05-01

    This research program will convert acid pretreated corn stover to sugars at the National Renewable Energy Laboratory (NREL) and then transfer these sugars to Honda R&D and its partner the Green Earth Institute (GEI) for conversion to ethanol via a novel fermentation organism. In phase one, NREL will adapt its pretreatment and saccharification process to the unique attributes of this organism, and Honda R&D/GEI will increase the sugar conversion rate as well as the yield and titer of the resulting ethanol. In later phases, NREL, Honda R&D, and GEI will work together at NREL to optimize and scale-up to pilot-scale the Honda R&D/GEI bioethanol production process. The final stage will be to undertake a pilot-scale test at NREL of the optimized bioethanol conversion process.

  8. FINAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    Horn, Mark W; McDaniel, Anthony; Schweighardt, Frank K

    2003-05-23

    In this program the teams at Penn State University (PSU), Sandia National Laboratories (SNL), DCH Technology (DCHT), and Air Products and Chemicals Inc. (APCI), have aggressively pursued engineering solutions to eliminate barriers to solid-state chemiresistor hydrogen sensor technology. The metallurgical effects of alloying palladium with nickel have been shown to prevent phase transitions in the thin films at high H2 overpressures, making the devices more suitable for IOF process conditions. We investigated the use of thin, semi-permeable membranes that protect the catalytic surface from poisoning or other undesirable surface reactions that would otherwise reduce sensitivity or operability in harsh IOF process environments. The results of this project have provided new insight into the effects of metallurgy and protective coatings on device behavior, and open new avenues for research in this field. Commercialization of this sensor technology could be easily achieved, although not yet realized. The benefits to society, once this technology is commercialized, is a dramatic cost and energy savings to the industry, which employs these sensors. In addition, the fundamental understandings gained in this program could have an impact on both cost and safety in the future hydrogen economy utilizing hydrogen fuel cells and hydrogen storage.

  9. The Wind Forecast Improvement Project (WFIP): A Public/Private Partnership for Improving Short Term Wind Energy Forecasts and Quantifying the Benefits of Utility Operations. The Southern Study Area, Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Freedman, Jeffrey M. [AWS Truepower, LLC, Albany, NY (United States); Manobianco, John [MESO, Inc., Troy, NY (United States); Schroeder, John [Texas Tech Univ., Lubbock, TX (United States). National Wind Inst.; Ancell, Brian [Texas Tech Univ., Lubbock, TX (United States). Atmospheric Science Group; Brewster, Keith [Univ. of Oklahoma, Norman, OK (United States). Center for Analysis and Prediction of Storms; Basu, Sukanta [North Carolina State Univ., Raleigh, NC (United States). Dept. of Marine, Earth, and Atmospheric Sciences; Banunarayanan, Venkat [ICF International (United States); Hodge, Bri-Mathias [National Renewable Energy Lab. (NREL), Golden, CO (United States); Flores, Isabel [Electricity Reliability Council of Texas (United States)

    2014-04-30

    This Final Report presents a comprehensive description, findings, and conclusions for the Wind Forecast Improvement Project (WFIP) -- Southern Study Area (SSA) work led by AWS Truepower (AWST). This multi-year effort, sponsored by the Department of Energy (DOE) and National Oceanographic and Atmospheric Administration (NOAA), focused on improving short-term (15-minute - 6 hour) wind power production forecasts through the deployment of an enhanced observation network of surface and remote sensing instrumentation and the use of a state-of-the-art forecast modeling system. Key findings from the SSA modeling and forecast effort include: 1. The AWST WFIP modeling system produced an overall 10 - 20% improvement in wind power production forecasts over the existing Baseline system, especially during the first three forecast hours; 2. Improvements in ramp forecast skill, particularly for larger up and down ramps; 3. The AWST WFIP data denial experiments showed mixed results in the forecasts incorporating the experimental network instrumentation; however, ramp forecasts showed significant benefit from the additional observations, indicating that the enhanced observations were key to the model systems’ ability to capture phenomena responsible for producing large short-term excursions in power production; 4. The OU CAPS ARPS simulations showed that the additional WFIP instrument data had a small impact on their 3-km forecasts that lasted for the first 5-6 hours, and increasing the vertical model resolution in the boundary layer had a greater impact, also in the first 5 hours; and 5. The TTU simulations were inconclusive as to which assimilation scheme (3DVAR versus EnKF) provided better forecasts, and the additional observations resulted in some improvement to the forecasts in the first 1 - 3 hours.

  10. FINAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    PETER, GARY F. [UNIVERSITY OF FLORIDA

    2014-07-16

    Excellent progress was made in standardizing three complementary methods: Magnetic resonance imaging, x-ray micro CT, and MALDI imaging linear ion trap mass spectroscopy to image biomass and chemical, anatomical and functional changes that occur during pretreatment and hydrolysis. Magnetic resonance microscopy provides excellent images with as low as 5 uM resolution with hydrated biomass samples. We visualized dramatic changes in signal associated with the hydrolysis of the carbohydrates by strong acids. Quantitative diffusion approaches were used to probe more subtle structural changes in biomass. Diffusion tensor calculations reflect diffusion anisotropy and fractional anisotropy maps clearly show the longer range diffusion within the vessels compared to within the fiber cells. The diffusion is increased along the cell walls of the vessels. Suggesting that further research with NMR imaging should be pursued. X-ray CT provides excellent images at as low as 3.5 uM resolution from dried biomass. Small increases in surface area, and decreases in local density have been quantified in with wood after mild pretreatments; these changes are expected to be underestimates of the hydrated wood, due to the ~12% shrinkage that occurs upon drying untreated wood. MALDI-MS spectra show high ion intensities at most mass to charge ratios in untreated and pretreated woody material. MALDI-MSn is required to improve specificity and reduce background for imaging. MALDI-TOF is not specific enough for carbohydrate identification. Using MALDI-LIT/MSn we can readily identify oligomeric glucans and xylans and their fragmentation patterns as well as those of the glucuronic acid side chains of birch 4-O-methyl glucuronxylan. Imaging of glucan and xylan oligomers show that many contain isobaric ions with different distributions, indicating again that MSn is needed for accurate imaging of lignocellulosic materials. We are now starting to integrate the three imaging methods by using the same set

  11. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Webb, Robert C. [Texas A& M University; Kamon, Teruki [Texas A& M University; Toback, David [Texas A& M University; Safonov, Alexei [Texas A& M University; Dutta, Bhaskar [Texas A& M University; Dimitri, Nanopoulos [Texas A& M University; Pope, Christopher [Texas A& M University; White, James [Texas A& M University

    2013-11-18

    Overview The High Energy Physics Group at Texas A&M University is submitting this final report for our grant number DE-FG02-95ER40917. This grant has supported our wide range of research activities for over a decade. The reports contained here summarize the latest work done by our research team. Task A (Collider Physics Program): CMS & CDF Profs. T. Kamon, A. Safonov, and D. Toback co-lead the Texas A&M (TAMU) collider program focusing on CDF and CMS experiments. Task D: Particle Physics Theory Our particle physics theory task is the combined effort of Profs. B. Dutta, D. Nanopoulos, and C. Pope. Task E (Underground Physics): LUX & NEXT Profs. R. Webb and J. White(deceased) lead the Xenon-based underground research program consisting of two main thrusts: the first, participation in the LUX two-phase xenon dark matter search experiment and the second, detector R&D primarily aimed at developing future detectors for underground physics (e.g. NEXT and LZ).

  12. Multiattribute Utility Theory without Expected Utility Foundations

    NARCIS (Netherlands)

    J. Miyamoto (John); P.P. Wakker (Peter)

    1996-01-01

    textabstractMethods for determining the form of utilities are needed for the implementation of utility theory in specific decisions. An important step forward was achieved when utility theorists characterized useful parametric families of utilities and simplifying decompositions of multiattribute ut

  13. Effects of partial replacement of dietary starch from barley or corn with lactose on ruminal function, short-chain fatty acid absorption, nitrogen utilization, and production performance of dairy cows.

    Science.gov (United States)

    Chibisa, G E; Gorka, P; Penner, G B; Berthiaume, R; Mutsvangwa, T

    2015-04-01

    In cows fed diets based on corn-alfalfa silage, replacing starch with sugar improves milk production. Although the rate of ruminal fermentation of sugar is more rapid than that of starch, evidence has been found that feeding sugar as a partial replacement for starch does not negatively affect ruminal pH despite increasing diet fermentability. The mechanism(s) for this desirable response are unknown. Our objective was to determine the effects of replacing barley or corn starch with lactose (as dried whey permeate; DWP) on ruminal function, short-chain fatty acid (SCFA) absorption, and nitrogen (N) utilization in dairy cows. Eight lactating cows were used in a replicated 4 × 4 Latin square design with 28-d periods and source of starch (barley vs. corn) and level of DWP (0 vs. 6%, DM basis) as treatment factors. Four cows in 1 Latin square were ruminally cannulated for the measurement of ruminal function, SCFA absorption, and N utilization. Dry matter intake and milk and milk component yields did not differ with diet. The dietary addition of DWP tended to increase ruminal butyrate concentration (13.6 vs. 12.2 mmol/L), and increased the Cl(-)-competitive absorption rates for acetate and propionate. There was no sugar effect on minimum ruminal pH, and the duration and area when ruminal pH was below 5.8. Minimum ruminal pH tended to be lower in cows fed barley compared with those fed corn (5.47 vs. 5.61). The duration when ruminal pH was below pH 5.8 tended to be shorter (186 vs. 235 min/d), whereas the area (pH × min/d) that pH was below 5.8 was smaller (47 vs. 111) on the corn than barley diets. Cows fed the high- compared with the low-sugar diet had lower ruminal NH3-N concentration. Feeding the high-sugar diet tended to increase apparent total-tract digestibility of dry matter and organic matters and increased apparent total-tract digestibility of fat. Apparent total-tract digestibility of N tended to be greater in cows fed barley compared with those fed corn

  14. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    J. K. Blasie; W.F. DeGrado; J.G. Saven; M.J. Therien

    2012-05-24

    The overall objective is to create robust artificial protein modules as scaffolds to control both (a) the conformation of novel cofactors incorporated into the modules thereby making the modules possess a desired functionality and (b) the organization of these functional modules into ordered macroscopic ensembles, whose macroscopic materials properties derive from the designed microscopic function of the modules. We focus on two specific types of cofactors for imparting functionality in this project; primarily nonlinear optical (NLO) chromophores designed to exhibit extraordinary molecular hyperpolarizabilities, as well as donor-bridge-acceptor cofactors designed to exhibit highly efficient, 'through-bonds' light-induced electron transfer (LIET) over nano-scale distances. The ensembles range from 2-D to 3-D, designed to possess the degree of orientational and positional order necessary to optimize their macroscopic response, the latter ranging from liquid-crystalline or glass-like to long-range periodic. Computational techniques, firmly based in statistical thermodynamics, are utilized for the design the artificial protein modules, based on robust {alpha}-helical bundle motifs, necessarily incorporating the desired conformation, location, and environment of the cofactor. Importantly, this design approach also includes optimization of the interactions between the modules to promote their organization into ordered macroscopic ensembles in 2-D and 3-D via either directed-assembly or self-assembly. When long-range periodic order is required, the design can be optimized to result a specified lattice symmetry. The structure and functionality of the individual modules are fully characterized at the microscopic level, as well as that of the ensembles at the macroscopic level, employing modern experimental physical-chemical and computational techniques. These include, for example, multi-dimensional NMR, various pump-probe transient spectroscopies to ultrafast time

  15. FINAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    Juergen Eckert; Anthony K. Cheetham (Principal Investigator)

    2011-03-11

    Hydrogen storage systems based on the readily reversible adsorption of H{sub 2} in porous materials have a number of very attractive properties with the potential to provide superior performance among candidate materials currently being investigated were it not for the fact that the interaction of H{sub 2} with the host material is too weak to permit viable operation at room temperature. Our study has delineated in quantitative detail the structural elements which we believe to be the essential ingredients for the future synthesis of porous materials, where guest-host interactions are intermediate between those found in the carbons and the metal hydrides, i.e. between physisorption and chemisorption, which will result in H{sub 2} binding energies required for room temperature operation. The ability to produce porous materials with much improved hydrogen binding energies depends critically on detailed molecular level analysis of hydrogen binding in such materials. However, characterization of H{sub 2} sorption is almost exclusively carried by thermodynamic measurements, which give average properties for all the sites occupied by H{sub 2} molecules at a particular loading. We have therefore extensively utilized the most powerful of the few molecular level experimental probes available to probe the interactions of hydrogen with porous materials, namely inelastic neutron scattering (INS) spectroscopy of the hindered rotations of the hydrogen molecules adsorbed at various sites, which in turn can be interpreted in a very direct way in by computational studies. This technique can relate spectral signatures of various H{sub 2} molecules adsorbed at binding sites with different degrees of interaction. In the course of this project we have synthesized a rather large number of entirely new hybrid materials, which include structural modifications for improved interactions with adsorbed hydrogen. The results of our systematic studies on many porous materials provide detailed

  16. Comparison of the utility of the classic model (the Henderson-Hasselbach equation) and the Stewart model (Strong Ion Approach) for the diagnostics of acid-base balance disorders in dogs with right sided heart failure.

    Science.gov (United States)

    Sławuta, P; Glińska-Suchocka, K

    2012-01-01

    Classically, the acid-base balance (ABB) is described by the Henderson-Hasselbach equation, where the blood pH is a result of a metabolic components--the HCO3(-) concentration and a respiratory component--pCO2. The Stewart model assumes that the proper understanding of the organisms ABB is based on an analysis of: pCO2, Strong Ion difference (SID)--the difference strong cation and anion concentrations in the blood serum, and the Acid total (Atot)--the total concentration of nonvolatile weak acids. Right sided heart failure in dogs causes serious haemodynamic disorders in the form of peripheral stasis leading to formation of transudates in body cavities, which in turn causes ABB respiratory and metabolic disorders. The study was aimed at analysing the ABB parameters with the use of the classic method and the Stewart model in dogs with the right sided heart failure and a comparison of both methods for the purpose of their diagnostic and therapeutic utility. The study was conducted on 10 dogs with diagnosed right sided heart failure. Arterial and venous blood was drawn from the animals. Analysis of pH, pCO2 and HCO3(-) was performed from samples of arterial blood. Concentrations of Na+, K+, Cl(-), P(inorganic), albumins and lactate were determined from venous blood samples and values of Strong Ion difference of Na+, K+ and Cl(-) (SID3), Strong Ion difference of Na+, K+, Cl(-) and lactate (SID4), Atot, Strong Ion difference effective (SIDe) and Strong Ion Gap (SIG4) were calculated. The conclusions are as follows: 1) diagnosis of ABB disorders on the basis of the Stewart model showed metabolic alkalosis in all dogs examined, 2) in cases of circulatory system diseases, methodology based on the Stewart model should be applied for ABB disorder diagnosis, 3) if a diagnosis of ABB disorders is necessary, determination of pH, pCO2 and HCO3(-) as well as concentrations of albumins and P(inorganic) should be determined on a routine basis, 4) for ABB disorder diagnosis, the

  17. Enhancement of light harvesting efficiency of silicon solar cell utilizing arrays of poly(methyl methacrylate-co-acrylic acid) nano-spheres and nano-spheres with embedded silver nano-particles

    Science.gov (United States)

    Lee, Chee-Leong; Goh, Wee-Sheng; Chee, Swee-Yong; Yik, Lai-Kuan

    2017-02-01

    An array of uniformly distributed monolayer of poly(methyl methacrylate-co-acrylic acid) nano-spheres were deposited onto an amorphous silicon photovoltaic cell utilizing dip coating technique. The electrical characteristics of the coated photovoltaic cell reveal that the nano-spheres with an average diameter size of 101 nm exhibits excellent light harvesting characteristics if compared to the nano-spheres of other sizes. The power conversion efficiency from such integration of the nano-structures (i.e. 3.14% per PV cell) indicates that at least 1.6 times of improvement (or relative enhancement of 57%) can be achieved comparatively to the uncoated photovoltaic cell (i.e. 2% per PV cell). Further increment of the power conversion efficiency of the solar cell has been attained with the incorporation of the silver nano-particles into the nano-spheres of similar average size. With the inclusion of the silver nano-particles into such nano-spheres, the power conversion efficiency of the solar cell has attained 5.57% per PV cell, which is about 2.8 times (or relative enhancement of 179%) if compared to the uncoated samples. Hence, this novel and controllable technique of fabricating omnidirectional light-harvesting nano-spheres with embedded silver nano-particles will indubitably be beneficial to various types of optoelectronic devices.

  18. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Weissman, Jon B

    2006-04-30

    computational resources in order to use the service, and the user need not be concerned with performance tuning. This can all be done by the service provider. We believe that the next dominant paradigm for high performance computing will be based on high-end network services. Putting high performance applications on-line will create a new generation of community services. Community services have several features which make their deployment challenging: (i) they must provide high performance, (ii) they are resource intensive, and (iii) they may be built upon a large existing code base. Many groups have built significant infrastructure for providing domain-specific high-end services [6][8][12][14][22][24][27][31][32]. However, this process is labor-intensive and time-consuming as evidenced by the development time required to build many of these systems. The reason is that these systems are all built from the ground-up with little existing infrastructure to utilize. Providing efficient, reliable, secure, and scalable services requires significant run-time infrastructure and middleware (Figure 1). The goal of this project is to develop general-purpose middleware to support the rapid deployment of high-end community services. In this proposal, we will focus on scalable middleware in support of resource management and reliability. We also propose a system architecture that integrates the middleware components. Our middleware and system architecture will be designed to accommodate and integrate middleware solutions for security and user interface1 developed by other groups. We will produce middleware that can be leveraged by community services running in clusters, supercomputers, and in Grids. One of the novel aspects of our approach is that the tension between resource sharing for the 'common good' and resource monopolization for the 'individual good' is significantly reduced. To increase the impact of this project, the middleware will be integrated into a widely

  19. Biological Function of Acetic Acid-Improvement in Obesity and Glucose Tolerance by Acetic Acid in Type 2 Diabetic Rats.

    Science.gov (United States)

    Yamashita, Hiromi

    2016-07-29

    Fatty acids derived from adipose tissue are oxidized by β-oxidation to form ketone bodies as final products under the starving condition. Previously, we found that free acetic acid was formed concomitantly with the production of ketone bodies in isolated rat liver perfusion, and mitochondrial acetyl CoA hydrolase was appeared to be involved with the acetic acid production. It was revealed that acetic acid was formed as a final product of enhanced β-oxidation of fatty acids and utilized as a fuel in extrahepatic tissues under the starving condition. Under the fed condition, β-oxidation is suppressed and acetic acid production is decreased. When acetic acid was taken daily by obesity-linked type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats under the fed condition, it protected OLETF rats against obesity. Furthermore, acetic acid contributed to protect from the accumulation of lipid in the liver as well as abdominal fat in OLETF rats. Transcripts of lipogenic genes in the liver were decreased, while transcripts of myoglobin and Glut4 genes in abdominal muscles were increased in the acetic acid-administered OLETF rats. It is indicated that exogenously administered acetic acid would have effects on lipid metabolism in both the liver and the skeletal muscles, and have function that works against obesity and obesity-linked type 2 diabetes.

  20. Advanced hydrogen/method utilization technology demonstration. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Lynch, F.; Fulton, J. [Hydrogen Consultants, Inc., Littleton, CO (United States)

    1994-04-01

    The overall objective of the work was to seek homogeneous blend ratios of hydrogen:methane that provide ``leverage`` with respect to exhaust emissions or engine performance. The leverage sought was a reduction in exhaust emissions or improved efficiency in proportions greater than the percentage of hydrogen energy in the blended fuel gas mixture. The scope of the study included the range of air/fuel mixtures from the lean limit to slightly richer than stoichiometric. This encompasses two important modes of engine operation for emissions control; lean burn pre-catalyst (some natural gas engines have no catalyst) and post-catalyst; and stoichiometric with three-way catalyst. The report includes a brief discussion of each of these modes.

  1. Geothermal Direct-Heat Utilization Assistance - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    J. W. Lund

    1999-07-14

    The Geo-Heat Center provided (1) direct-use technical assistance, (2) research, and (3) information dissemination on geothermal energy over an 8 1/2 year period. The center published a quarterly bulletin, developed a web site and maintained a technical library. Staff members made 145 oral presentations, published 170 technical papers, completed 28 applied research projects, and gave 108 tours of local geothermal installations to 500 persons.

  2. SPECIFICATION AND UTILIZATION OF A TRANSFORMATIONAL GRAMMAR. FINAL REPORT.

    Science.gov (United States)

    BLAIR, FRED; ROSENBAUM, PETER S.

    RESEARCH IN THREE AREAS OF COMPUTATIONAL LINGUISTICS IS DESCRIBED--(1) THE DESIGN AND DEVELOPMENT OF A TRANSFORMATIONAL GRAMMAR FOR A SUBSET TO GRAMMATICAL SENTENCES IN ENGLISH, (2) THE IMPLEMENTATION OF THIS GRAMMAR IN TERMS OF A SENTENCE SYNTHESIZING PROGRAM WRITTEN IN LISP 1.5, AND (3) THE USE OF SENTENCE SYNTHESIZING PROGRAMS FOR…

  3. Final Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Josef Michl

    2011-10-31

    In this project we have established guidelines for the design on organic chromophores suitable for producing high triplet yields via singlet fission. We have proven their utility by identifying a chromophore of a structural class that had never been examined for singlet fission before, 1,3-diphenylisobenzofuran, and demonstrating in two independent ways that a thin layer of this material produces a triplet yield of 200% within experimental error. We have also designed a second chromophore of a very different type, again of a structural class that had not been examined for singlet fission before, and found that in a thin layer it produces a 70% triplet yield. Finally, we have enhanced the theoretical understanding of the quantum mechanical nature of the singlet fission process.

  4. Expression and/or activity of the SVCT2 ascorbate transporter may be decreased in many aggressive cancers, suggesting potential utility for sodium bicarbonate and dehydroascorbic acid in cancer therapy.

    Science.gov (United States)

    McCarty, Mark F

    2013-10-01

    Hypoxia-inducible factor-1 (HIF-1) is a heterodimer transcription factor whose elevated activity in many cancers helps them to survive under hypoxic conditions and enhances their capacity to grow invasively, establish metastases, and survive chemo- or radiotherapy. Optimal intracellular levels of ascorbate suppress the level and transcriptional activity of HIF-1under normoxic or mildly hypoxic conditions by supporting the activity of proly and asparagyl hydroxylases that target HIF-1alpha. High intracellular ascorbate can also work in various ways to down-regulate activation of NF-kappaB which, like HIF-1 is constitutively active in many cancers and promotes aggressive behavior - in part by promoting transcription of HIF-1alpha. Yet recent evidence suggests that, even in the context of adequate ascorbate nutrition, the intracellular ascorbate content of many aggressive cancers may be supoptimal for effective HIF-1 control. This likely reflects low expression or activity of the SVCT2 ascorbate transporter. The expression of SVCT2 in cancers has so far received little study; but the extracellular acidity characteristic of many tumors would be expected to reduce the activity of this transporter, which has a mildly alkaline pH optimum. Unfortunately, since SVCT2 has a high affinity for ascorbate, and its activity is nearly saturated at normal healthy serum levels of this vitamin, increased oral administration of ascorbate would be unlikely to have much impact on the intracellular ascorbate content of tumors. However, cancers in which HIF-1 is active express high levels of glucose transporters such as GLUT-1, and these transporters can promote influx of dehydroascorbic acid (DHA) via facilitated diffusion; once inside the cell, DHA is rapidly reduced to ascorbate, which effectively is "trapped" within the cell. Hence, episodic intravenous infusions of modest doses of DHA may have potential for optimizing the intracellular ascorbate content of cancers, potentially

  5. 2,4-Dichlorophenoxyacetic acid (2,4-D) utilization by Delftia acidovorans MC1 at alkaline pH and in the presence of dichlorprop is improved by introduction of the tfdK gene.

    Science.gov (United States)

    Hoffmann, Doreen; Müller, Roland H

    2006-06-01

    Growth of Delftia acidovorans MC1 on 2,4-dichlorophenoxyacetic acid (2,4-D) and on racemic 2-(2,4-dichlorophenoxy)propanoic acid ((RS)-2,4-DP) was studied in the perspective of an extension of the strain's degradation capacity at alkaline pH. At pH 6.8 the strain grew on 2,4-D at a maximum rate (mu max) of 0.158 h(-1). The half-maximum rate-associated substrate concentration (Ks) was 45 microM. At pH 8.5 mu max was only 0.05 h(-1) and the substrate affinity was mucher lower than at pH 6.8. The initial attack of 2,4-D was not the limiting step at pH 8.5 as was seen from high dioxygenase activity in cells grown at this pH. High stationary 2,4-D concentrations and the fact that mu max with dichlorprop was around 0.2 h(-1) at both pHs rather pointed at limited 2,4-D uptake at pH 8.5. Introduction of tfdK from D. acidovorans P4a by conjugation, coding for a 2,4-D-specific transporter resulted in improved growth on 2,4-D at pH 8.5 with mu max of 0.147 h(-1) and Ks of 267 microM. Experiments with labeled substrates showed significantly enhanced 2,4-D uptake by the transconjugant TK62. This is taken as an indication of expression of the tfdK gene and proper function of the transporter. The uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP) reduced the influx of 2,4-D. At a concentration of 195 microM 2,4-D, the effect amounted to 90% and 50%, respectively, with TK62 and MC1. Cloning of tfdK also improved the utilization of 2,4-D in the presence of (RS)-2,4-DP. Simultaneous and almost complete degradation of both compounds occurred in TK62 up to D = 0.23 h(-1) at pH 6.8 and up to D = 0.2 h(-1) at pH 8.5. In contrast, MC1 left 2,4-D largely unutilized even at low dilution rates when growing on herbicide mixtures at pH 8.5.

  6. Isothermal Amplification of Nucleic Acids.

    Science.gov (United States)

    Zhao, Yongxi; Chen, Feng; Li, Qian; Wang, Lihua; Fan, Chunhai

    2015-11-25

    Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently accumulates nucleic acid sequences at constant temperature. Since the early 1990s, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR). These isothermal amplification methods have been used for biosensing targets such as DNA, RNA, cells, proteins, small molecules, and ions. The applications of these techniques for in situ or intracellular bioimaging and sequencing have been amply demonstrated. Amplicons produced by isothermal amplification methods have also been utilized to construct versatile nucleic acid nanomaterials for promising applications in biomedicine, bioimaging, and biosensing. The integration of isothermal amplification into microsystems or portable devices improves nucleic acid-based on-site assays and confers high sensitivity. Single-cell and single-molecule analyses have also been implemented based on integrated microfluidic systems. In this review, we provide a comprehensive overview of the isothermal amplification of nucleic acids encompassing work published in the past two decades. First, different isothermal amplification techniques are classified into three types based on reaction kinetics. Then, we summarize the applications of isothermal amplification in bioanalysis, diagnostics, nanotechnology, materials science, and device integration. Finally, several challenges and perspectives in the field are discussed.

  7. The final cool down

    CERN Multimedia

    Thursday 29th May, the cool-down of the final sector (sector 4-5) of LHC has begun, one week after the start of the cool-down of sector 1-2. It will take five weeks for the sectors to be cooled from room temperature to 5 K and a further two weeks to complete the cool down to 1.9 K and the commissioning of cryogenic instrumentation, as well as to fine tune the cryogenic plants and the cooling loops of cryostats.Nearly a year and half has passed since sector 7-8 was cooled for the first time in January 2007. For Laurent Tavian, AT/CRG Group Leader, reaching the final phase of the cool down is an important milestone, confirming the basic design of the cryogenic system and the ability to operate complete sectors. “All the sectors have to operate at the same time otherwise we cannot inject the beam into the machine. The stability and reliability of the cryogenic system and its utilities are now very important. That will be the new challenge for the coming months,” he explains. The status of the cool down of ...

  8. Production of green biocellulose nanofibers by Gluconacetobacter xylinus through utilizing the renewable resources of agriculture residues.

    Science.gov (United States)

    Al-Abdallah, Wahib; Dahman, Yaser

    2013-11-01

    The present study demonstrates the ability to produce green biocellulose nanofibers using the renewable resources of agriculture residues. Locally grown wheat straws (WS) were hydrolyzed under different conditions. Their hydrolysates were utilized to produce the nanofibers in separate hydrolysis fermentation process by Gluconacetobacter xylinus strain bacterium. Highest biocellulose production of ~10.6 g/L was achieved with samples that were enzymatically hydrolyzed. Moreover, acidic hydrolyzed WS produced up to 9.7 g/L, with total sugar concentrations in culture media of 43 g/L. Generally, enzymatic hydrolysis of WS resulted in more total sugar concentration than the acidic hydrolysis (i.e., 52.12 g/L), while water hydrolysis produced the least. This can be related to utilizing Xylanase in addition to Cellulase and Beta-glucosidase that helps to hydrolyse WS dry basis of cellulose and hemicelluloses. Sugar mixtures produced under all hydrolysis conditions were mainly composed of glucose and xylose with average percentages of 56 and 28 %, respectively. Acidic hydrolysis at higher acid concentration, as well as soaking WS in the acidic solution for longer time, improved the total sugar concentration in the culture media by 18 %. Conducting thermal treatment at more intense conditions of higher temperature or heating time improved the total sugar produced with acidic hydrolysis. These conditions, however, resulted in further production of furfural, which considerably affected bacterial cells proliferation. This resulted in lowest sugar consumption in the range of 62-64 % that affected final BC production.

  9. Nicotine, IFN-γ and retinoic acid mediated induction of MUC4 in pancreatic cancer requires E2F1 and STAT-1 transcription factors and utilize different signaling cascades

    Directory of Open Access Journals (Sweden)

    Kunigal Sateesh

    2012-04-01

    Full Text Available Abstract Background The membrane-bound mucins are thought to play an important biological role in cell–cell and cell–matrix interactions, in cell signaling and in modulating biological properties of cancer cell. MUC4, a transmembrane mucin is overexpressed in pancreatic tumors, while remaining undetectable in the normal pancreas, thus indicating a potential role in pancreatic cancer pathogenesis. The molecular mechanisms involved in the regulation of MUC4 gene are not yet fully understood. Smoking is strongly correlated with pancreatic cancer and in the present study; we elucidate the molecular mechanisms by which nicotine as well as agents like retinoic acid (RA and interferon-γ (IFN-γ induce the expression of MUC4 in pancreatic cancer cell lines CD18, CAPAN2, AsPC1 and BxPC3. Results Chromatin immunoprecipitation assays and real-time PCR showed that transcription factors E2F1 and STAT1 can positively regulate MUC4 expression at the transcriptional level. IFN-γ and RA could collaborate with nicotine in elevating the expression of MUC4, utilizing E2F1 and STAT1 transcription factors. Depletion of STAT1 or E2F1 abrogated the induction of MUC4; nicotine-mediated induction of MUC4 appeared to require α7-nicotinic acetylcholine receptor subunit. Further, Src and ERK family kinases also mediated the induction of MUC4, since inhibiting these signaling molecules prevented the induction of MUC4. MUC4 was also found to be necessary for the nicotine-mediated invasion of pancreatic cancer cells, suggesting that induction of MUC4 by nicotine and other agents might contribute to the genesis and progression of pancreatic cancer. Conclusions Our studies show that agents that can promote the growth and invasion of pancreatic cancer cells induce the MUC4 gene through multiple pathways and this induction requires the transcriptional activity of E2F1 and STAT1. Further, the Src as well as ERK signaling pathways appear to be involved in the induction of

  10. 皇竹草对酸与Cd污染农田土壤的治理效果及安全应用分析%Phytoremediation efficiency of Pennisetum hydridum for acid- and cadmium- polluted soil and its safe utilization

    Institute of Scientific and Technical Information of China (English)

    谢华; 赵雪梅; 谢洲; 吴开庆; 李相林; 杨瑞刚; 彭波; 余孟好; 何金华

    2016-01-01

    在大田条件下,研究了在酸与Cd污染农田土壤中种植皇竹草对污染农田的治理效果和应用安全性。结果表明,在各土壤处理中,对照处理(不施用土壤添加剂)的皇竹草中Cd含量最高,为1.69 mg·kg-1(干重),富集系数大于1,表现出一定的Cd富集特性。对照处理的皇竹草对Cd的提取量、提取效率分别为119.91 g·hm-2、6.98%,修复效果优于在同等条件下种植的Cd超富集植物龙葵,若将试验区的土壤Cd修复到《土壤环境质量标准》(GB 15618—1995)的二级标准限值以内,经计算约需9年。皇竹草既可以提取土壤中的Cd,同时还可作为饲用牧草、还田肥料、造纸原料、能源植物等进行安全利用。因此,皇竹草是治理修复及综合利用酸与Cd污染农田的可行性植物材料。%Under field conditions, phytoremediation of acid- and cadmium- polluted soil by and its application safety were studied. Pen-nisetum hydridum exhibited Cd-enrichment capacity, as indicated by its enrichment coefficient of greater than 1. Under different soil treat-ments, Cd content, extraction quantity and efficiency of P.hydridum were the highest in the control, which was up to 1.69 mg·kg-1(dry weigh), 119.91 g·hm-2 and 6.98%, respectively. This indicated that phytoremediation efficiency of P. hydridum was better than that of Solanumnigrum, a known Cd hyperaccumulator. Calculation showed that it would take only 9 years for P. hydridum to remedy Cd polluted soil in the experimental area by reducing soil Cd below the limit of the grade II of "Environmental Quality Standards for Soils"(GB 15618—1995). In addition to soil remediation, P. hydridum also has wide scope of applications, such as forage grass, organic fertilizer, paper-making material, energy plant, etc. Pennisetum hydridum would therefore be a feasible plant for phytoremediation of acid- and Cd-polluted farmland and safe utilization.

  11. Domestic utility attitudes toward foreign uranium supply

    Energy Technology Data Exchange (ETDEWEB)

    1981-06-01

    The current embargo on the enrichment of foreign-origin uranium for use in domestic utilization facilities is scheduled to be removed in 1984. The pending removal of this embargo, complicated by a depressed worldwide market for uranium, has prompted consideration of a new or extended embargo within the US Government. As part of its on-going data collection activities, Nuclear Resources International (NRI) has surveyed 50 domestic utility/utility holding companies (representing 60 lead operator-utilities) on their foreign uranium purchase strategies and intentions. The most recent survey was conducted in early May 1981. A number of qualitative observations were made during the course of the survey. The major observations are: domestic utility views toward foreign uranium purchase are dynamic; all but three utilities had some considered foreign purchase strategy; some utilities have problems with buying foreign uranium from particular countries; an inducement is often required by some utilities to buy foreign uranium; opinions varied among utilities concerning the viability of the domestic uranium industry; and many utilities could have foreign uranium fed through their domestic uranium contracts (indirect purchases). The above observations are expanded in the final section of the report. However, it should be noted that two of the observations are particularly important and should be seriously considered in formulation of foreign uranium import restrictions. These important observations are the dynamic nature of the subject matter and the potentially large and imbalanced effect the indirect purchases could have on utility foreign uranium procurement.

  12. Utility usage forecasting

    Energy Technology Data Exchange (ETDEWEB)

    Hosking, Jonathan R. M.; Natarajan, Ramesh

    2017-08-22

    The computer creates a utility demand forecast model for weather parameters by receiving a plurality of utility parameter values, wherein each received utility parameter value corresponds to a weather parameter value. Determining that a range of weather parameter values lacks a sufficient amount of corresponding received utility parameter values. Determining one or more utility parameter values that corresponds to the range of weather parameter values. Creating a model which correlates the received and the determined utility parameter values with the corresponding weather parameters values.

  13. Entrez Programming Utilities

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Entrez Programming Utilities (E-utilities) are a set of eight server-side programs that provide a stable interface into the Entrez query and database system at...

  14. Utility Solar Generation Valuation Methods

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, Thomas N.; Dion, Phillip J.

    2009-06-30

    Tucson Electric Power (TEP) developed, tested and verified the results of a new and appropriate method for accurately evaluating the capacity credit of time variant solar generating sources and reviewed new methods to appropriately and fairly evaluate the value of solar generation to electric utilities. The project also reviewed general integrated approaches for adequately compensating owners of solar generation for their benefits to utilities. However, given the limited funding support and time duration of this project combined with the significant differences between utilities regarding rate structures, solar resource availability and coincidence of solar generation with peak load periods, it is well beyond the scope of this project to develop specific rate, rebate, and interconnection approaches to capture utility benefits for all possible utilities. The project developed computer software based evaluation method models to compare solar generation production data measured in very short term time increments called Sample Intervals over a typical utility Dispatch Cycle during an Evaluation Period against utility system load data. Ten second resolution generation production data from the SGSSS and actual one minute resolution TEP system load data for 2006 and 2007, along with data from the Pennington Street Garage 60 kW DC capacity solar unit installed in downtown Tucson will be applied to the model for testing and verification of the evaluation method. Data was provided by other utilities, but critical time periods of data were missing making results derived from that data inaccurate. The algorithms are based on previous analysis and review of specific 2005 and 2006 SGSSS production data. The model was built, tested and verified by in house TEP personnel. For this phase of the project, TEP communicated with, shared solar production data with and collaborated on the development of solar generation valuation tools with other utilities, including Arizona Public

  15. Recent advances in lactic acid production by microbial fermentation processes.

    Science.gov (United States)

    Abdel-Rahman, Mohamed Ali; Tashiro, Yukihiro; Sonomoto, Kenji

    2013-11-01

    Fermentative production of optically pure lactic acid has roused interest among researchers in recent years due to its high potential for applications in a wide range of fields. More specifically, the sharp increase in manufacturing of biodegradable polylactic acid (PLA) materials, green alternatives to petroleum-derived plastics, has significantly increased the global interest in lactic acid production. However, higher production costs have hindered the large-scale application of PLA because of the high price of lactic acid. Therefore, reduction of lactic acid production cost through utilization of inexpensive substrates and improvement of lactic acid production and productivity has become an important goal. Various methods have been employed for enhanced lactic acid production, including several bioprocess techniques facilitated by wild-type and/or engineered microbes. In this review, we will discuss lactic acid producers with relation to their fermentation characteristics and metabolism. Inexpensive fermentative substrates, such as dairy products, food and agro-industrial wastes, glycerol, and algal biomass alternatives to costly pure sugars and food crops are introduced. The operational modes and fermentation methods that have been recently reported to improve lactic acid production in terms of concentrations, yields, and productivities are summarized and compared. High cell density fermentation through immobilization and cell-recycling techniques are also addressed. Finally, advances in recovery processes and concluding remarks on the future outlook of lactic acid production are presented.

  16. Processing and Utilization of Naphthenic Base Heavy Crude Oil (continued)

    Institute of Scientific and Technical Information of China (English)

    Wang Xianqing; Men Cungui

    1995-01-01

    @@ Strategy of the Comprehensive Utilization of Naphthenic Base Heavy Crude The further study on the properties and characteristics of the naphthenic base heavy crude shows that the utilization of naphthenic acid, production of low freezing point lube stocks and a series of asphalts are important future trends for comprehensive utilization of these resources.

  17. Zymomonas with improved xylose utilization in stress conditions

    Energy Technology Data Exchange (ETDEWEB)

    Caimi, Perry G; Emptage, Mark; Li, Xu; Viitanen, Paul V; Chou, Yat-Chen; Franden, Mary Ann; Zhang, Min

    2013-06-18

    Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.

  18. Tiger LDRD final report

    Energy Technology Data Exchange (ETDEWEB)

    Steich, D J; Brugger, S T; Kallman, J S; White, D A

    2000-02-01

    This final report describes our efforts on the Three-Dimensional Massively Parallel CEM Technologies LDRD project (97-ERD-009). Significant need exists for more advanced time domain computational electromagnetics modeling. Bookkeeping details and modifying inflexible software constitute a vast majority of the effort required to address such needs. The required effort escalates rapidly as problem complexity increases. For example, hybrid meshes requiring hybrid numerics on massively parallel platforms (MPPs). This project attempts to alleviate the above limitations by investigating flexible abstractions for these numerical algorithms on MPPs using object-oriented methods, providing a programming environment insulating physics from bookkeeping. The three major design iterations during the project, known as TIGER-I to TIGER-III, are discussed. Each version of TIGER is briefly discussed along with lessons learned during the development and implementation. An Application Programming Interface (API) of the object-oriented interface for Tiger-III is included in three appendices. The three appendices contain the Utilities, Entity-Attribute, and Mesh libraries developed during the project. The API libraries represent a snapshot of our latest attempt at insulated the physics from the bookkeeping.

  19. Nonexpected utility and coherence

    NARCIS (Netherlands)

    Diecidue, E.

    2001-01-01

    The descriptive power of expected utility has been challenged by behavioral evidence showing that people deviate systematically from the expected utility paradigm. Since the end of the 70's several alternatives to the classical expected utility paradigm have been proposed in order to accommodate the

  20. Information, evolution and utility

    Directory of Open Access Journals (Sweden)

    Larry Samuelson

    2006-03-01

    Full Text Available Human utility embodies a number of seemingly irrational aspects. The leading example in this paper is that utilities often depend on the presence of salient unchosen alternatives. Our focus is to understand why an evolutionary process might optimally lead to such seemingly dysfunctional features in our motivations and to derive implications for the nature of our utility functions.

  1. Microorganism Utilization for Synthetic Milk

    Science.gov (United States)

    Morford, Megan A.; Khodadad, Christina L.; Caro, Janicce I.; Spencer, LaShelle E.; Richards, Jeffery T.; Strayer, Richard F.; Birmele, Michele N.; Wheeler, Raymond M.

    2014-01-01

    A desired architecture for long duration spaceflight, like aboard the International Space Station or for future missions to Mars, is to provide a supply of fresh food crops for the astronauts. However, some crops can create a high proportion of inedible plant waste. The main goal of the Synthetic Biology project, Cow in a Column, was to produce the components of milk (sugar, lipid, protein) from inedible plant waste by utilizing microorganisms (fungi, yeast, bacteria). Of particular interest was utilizing the valuable polysaccharide, cellulose, found in plant waste, to naturally fuel-through microorganism cellular metabolism- the creation of sugar (glucose), lipid (milk fat), and protein (casein) in order to produce a synthetic edible food product. Environmental conditions such as pH, temperature, carbon source, aeration, and choice microorganisms were optimized in the laboratory and the desired end-products, sugars and lipids, were analyzed. Trichoderma reesei, a known cellulolytic fungus, was utilized to drive the production of glucose, with the intent that the produced glucose would serve as the carbon source for milk fat production and be a substitute for the milk sugar lactose. Lipid production would be carried out by Rhodosporidium toruloides, yeast known to accumulate those lipids that are typically found in milk fat. Results showed that glucose and total lipid content were below what was expected during this phase of experimentation. In addition, individual analysis of six fatty acids revealed that the percentage of each fatty acid was lower than naturally produced bovine milk. Overall, this research indicates that microorganisms could be utilized to breakdown inedible solid waste to produce useable products. For future work, the production of the casein protein for milk would require the development of a genetically modified organism, which was beyond the scope of the original project. Additional trials would be needed to further refine the required

  2. Comparison of the utility of the classic model (the Henderson-Hasselbach equation) and the Stewart model (Strong Ion Approach) for the diagnostics of acid-base balance disorders in dogs with right sided heart failure

    National Research Council Canada - National Science Library

    P. Sławuta; K. Glińska-Suchocka

    2012-01-01

    Classically, the acid-base balance (ABB) is described by the Henderson-Hasselbach equation, where the blood pH is a result of a metabolic components - the HCO concentration and a respiratory component - pCO...

  3. 1995 PVUSA progress report. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    Photovoltaics for Utility Scale Applications (PVUSA) is a national public-private partnership that is assessing and demonstrating the viability of utility-scale (US) photovoltaic (PV) electric generation systems and recent developments in PV module technology. This report updates the project`s progress, reviews the status and performance of the various PV installations during 1995, summarizes key accomplishments and conclusions, and serves as the final report under Pacific Gas and Electric Company`s project management.

  4. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Aristos Aristidou Natureworks); Robert Kean (NatureWorks); Tom Schechinger (IronHorse Farms, Mat); Stuart Birrell (Iowa State); Jill Euken (Wallace Foundation & Iowa State)

    2007-10-01

    The two main objectives of this project were: 1) to develop and test technologies to harvest, transport, store, and separate corn stover to supply a clean raw material to the bioproducts industry, and 2) engineer fermentation systems to meet performance targets for lactic acid and ethanol manufacturers. Significant progress was made in testing methods to harvest corn stover in a “single pass” harvest mode (collect corn grain and stover at the same time). This is technically feasible on small scale, but additional equipment refinements will be needed to facilitate cost effective harvest on a larger scale. Transportation models were developed, which indicate that at a corn stover yield of 2.8 tons/acre and purchase price of $35/ton stover, it would be unprofitable to transport stover more than about 25 miles; thus suggesting the development of many regional collection centers. Therefore, collection centers should be located within about 30 miles of the farm, to keep transportation costs to an acceptable level. These collection centers could then potentially do some preprocessing (to fractionate or increase bulk density) and/or ship the biomass by rail or barge to the final customers. Wet storage of stover via ensilage was tested, but no clear economic advantages were evident. Wet storage eliminates fire risk, but increases the complexity of component separation and may result in a small loss of carbohydrate content (fermentation potential). A study of possible supplier-producer relationships, concluded that a “quasi-vertical” integration model would be best suited for new bioproducts industries based on stover. In this model, the relationship would involve a multiyear supply contract (processor with purchase guarantees, producer group with supply guarantees). Price will likely be fixed or calculated based on some formula (possibly a cost plus). Initial quality requirements will be specified (but subject to refinement).Producers would invest in harvest

  5. Design and characterization of auxotrophy-based amino acid biosensors.

    Directory of Open Access Journals (Sweden)

    Felix Bertels

    Full Text Available Efficient and inexpensive methods are required for the high-throughput quantification of amino acids in physiological fluids or microbial cell cultures. Here we develop an array of Escherichia coli biosensors to sensitively quantify eleven different amino acids. By using online databases, genes involved in amino acid biosynthesis were identified that - upon deletion - should render the corresponding mutant auxotrophic for one particular amino acid. This rational design strategy suggested genes involved in the biosynthesis of arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan, and tyrosine as potential genetic targets. A detailed phenotypic characterization of the corresponding single-gene deletion mutants indeed confirmed that these strains could neither grow on a minimal medium lacking amino acids nor transform any other proteinogenic amino acid into the focal one. Site-specific integration of the egfp gene into the chromosome of each biosensor decreased the detection limit of the GFP-labeled cells by 30% relative to turbidometric measurements. Finally, using the biosensors to determine the amino acid concentration in the supernatants of two amino acid overproducing E. coli strains (i.e. ΔhisL and ΔtdcC both turbidometrically and via GFP fluorescence emission and comparing the results to conventional HPLC measurements confirmed the utility of the developed biosensor system. Taken together, our study provides not only a genotypically and phenotypically well-characterized set of publicly available amino acid biosensors, but also demonstrates the feasibility of the rational design strategy used.

  6. The marginal efficiency of utilization of all ileal digestible indispensable amino acids for protein gain is lower than 30% in preruminant calves between 80 and 240 kg live weight

    NARCIS (Netherlands)

    Gerrits, W.J.J.; Schrama, J.W.; Tamminga, S.

    1998-01-01

    A previous study showed that the marginal efficiency of utilization of digestible nitrogen for deposition in the body in preruminant calves is only ~30%. The study consisted of two similar experiments that were performed in two live weight ranges: 80-160 and 160-240 kg. In each experiment, 36 calves

  7. Utilize Vacuum Forming to Make Interdisciplinary Connections

    Science.gov (United States)

    Love, Tyler S.; Valenza, Frank

    2011-01-01

    The concept of vacuum forming has been around since the 19th century, despite not being fully utilized in industry until the 1950s. In the past, industrial arts classes have used vacuum-forming projects to concentrate solely on the manufacturing process and the final product. However, vacuum forming is not just an old industrial arts activity; it…

  8. Criterions preparation and characterization of earthworm-composts in view of animal waste recycling: Part II. A synergistic utilization of EPR and {sup 1}H NMR spectroscopies on the characterization of humic acids from vermi composts

    Energy Technology Data Exchange (ETDEWEB)

    Guimaraes, Elisete [Centro Federal de Educacao Tecnologica, Pato Branco, PR (Brazil); Mangrich, Antonio S.; Machado, Vanderlei G. [Parana Univ., Curitiba, PR (Brazil). Dept. de Quimica]. E-mail: mangrich@quimica.ufpr.br; Traghetta, Dinis G.; Lobo, Maria A. [Centro Universitario Positivo, Curitiba, PR (Brazil)

    2001-12-01

    Humic acids (HA) extracted from sheep (SHHA), cow (COHA), goat (GOHA) and rabbit (RAHA) vermi composted manure were analyzed by electron paramagnetic resonance and hydrogen nuclear magnetic resonance spectroscopies. Carboxylic acids, amine, amide, ester, ether and phenol functions bonded to saturated aliphatic, unsaturated aliphatic conjugated double and single bonds, and aromatic chains constitute the backbone structure of these fresh humic substances (H S). Mn {sup 2+} outer sphere complexes (SHHA, COHA), Fe{sup 3+} axial (COHA, RAHA) or rhombic (SHAHA, COHA, GOHA, RAHA) complexes and Cu{sup 2+} as weak field (COHA, GOHA, RAHA) and strong field (SHAHA, COHA, GOHA, RAHA) complexes were characterized. (author)

  9. Criterious Preparation and Characterization of Earthworm-composts in View of Animal Waste Recycling: Part II. A Synergistic Utilization of EPR and 1H NMR Spectroscopies on the Characterization of Humic Acids from Vermicomposts

    Directory of Open Access Journals (Sweden)

    Guimarães Elisete

    2001-01-01

    Full Text Available Humic acids (HA extracted from sheep (SHHA, cow (COHA, goat (GOHA and rabbit (RAHA vermicomposted manure were analyzed by electron paramagnetic resonance and hydrogen nuclear magnetic resonance spectroscopies. Carboxylic acids, amine, amide, ester, ether and phenol functions bonded to saturated aliphatic, unsaturated aliphatic conjugated double and single bonds, and aromatic chains constitute the backbone structure of these fresh humic substances (HS. Mn2+ outer sphere complexes (SHHA, COHA, Fe3+ axial (COHA, RAHA or rhombic (SHAHA, COHA, GOHA, RAHA complexes and Cu2+ as weak field (COHA, GOHA, RAHA and strong field (SHAHA, COHA, GOHA, RAHA complexes were characterized.

  10. 酸碱联产再生循环与菱镁矿综合利用耦合新工艺%New Technology of Acid-alkali Co-production and Regenerative Cycle Coupled With Comprehensive Utilization of Magnesite

    Institute of Scientific and Technical Information of China (English)

    王云山; 杨刚; 沈克兵; 惠迎花; 张金平; 安学斌

    2015-01-01

    The new technology of acid‐alkali co‐production and regenerative cycle coupled with comprehensive utilization of magnesite were studied .The suitable process parameters for acid‐leaching ,hydrolysis and ammonia evaporation w ere proposed .T he new technology needn't buy acid and alkali theoretically ,the energy can be utilized reasonable and stairstep ,and the solid waste residue can be used to produce building materials .T he new technology is green ,clean ,higher resource utilization and energy‐saving and cost‐reducing .T he new technology has observably economic ,environmental and social benefit ,and provides a new way for comprehensive utilization of magnesite .%研究了酸、碱联产再生循环与菱镁矿资源综合开发利用有机耦合新工艺,提出酸浸、水解及蒸氨适宜工艺参数。新工艺理论上无需外购酸、碱,能量可合理梯级利用,固体废渣可用于生产建材,具有绿色、清洁、环保、资源利用率高、节能降耗等特点,有明显的经济效益、环境效益和社会效益,是菱镁矿资源综合利用的新途径。

  11. Public utility regulation and national energy policy

    Energy Technology Data Exchange (ETDEWEB)

    Navarro, P.

    1980-09-01

    The linkage between Public Utility Commission (PUC) regulation, the deteriorating financial health of the electric utility industry, and implementation of national energy policy, particularly the reduction of foreign petroleum consumption in the utility sector is examined. The role of the Nation's utilities in the pursuit of national energy policy goals and postulates a linkage between PUC regulation, the poor financial health of the utility industry, and the current and prospective failure to displace foreign petroleum in the utility sector is discussed. A brief history of PUC regulation is provided. The concept of regulatory climate and how the financial community has developed a system of ranking regulatory climate in the various State jurisdictions are explained. The existing evidence on the hypothesis that the cost of capital to a utility increases and its availability is reduced as regulatory climate grows more unfavorable from an investor's point of view is analyzed. The implications of this cost of capital effect on the electric utilities and collaterally on national energy policy and electric ratepayers are explained. Finally various State, regional and Federal regulatory responses to problems associated with PUC regulation are examined.

  12. Working memory management and predicted utility.

    Science.gov (United States)

    Chatham, Christopher H; Badre, David

    2013-01-01

    Given the limited capacity of working memory (WM), its resources should be allocated strategically. One strategy is filtering, whereby access to WM is granted preferentially to items with the greatest utility. However, reallocation of WM resources might be required if the utility of maintained information subsequently declines. Here, we present behavioral, computational, and neuroimaging evidence that human participants track changes in the predicted utility of information in WM. First, participants demonstrated behavioral costs when the utility of items already maintained in WM declined and resources should be reallocated. An adapted Q-learning model indicated that these costs scaled with the historical utility of individual items. Finally, model-based neuroimaging demonstrated that frontal cortex tracked the utility of items to be maintained in WM, whereas ventral striatum tracked changes in the utility of items maintained in WM to the degree that these items are no longer useful. Our findings suggest that frontostriatal mechanisms track the utility of information in WM, and that these dynamics may predict delays in the removal of information from WM.

  13. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Schuur, Edward [Northern Arizona Univ., Flagstaff, AZ (United States); Luo, Yiqi [Univ. of Oklahoma, Norman, OK (United States)

    2016-12-01

    This final grant report is a continuation of the final grant report submitted for DE-SC0006982 as the Principle Investigator (Schuur) relocated from the University of Florida to Northern Arizona University. This report summarizes the original project goals, as well as includes new project activities that were completed in the final period of the project.

  14. Silicon Utilizing Microbial Bioactivities in the Biosphere

    Science.gov (United States)

    Sen, M. M.; Das, S.

    2012-12-01

    Diatoms are unicellular eukaryotic algae and an important member of the silicon utilizing organisms, that generate ~20% of the ~100 billion metric tons of organic carbon produced through photosynthesis on Earth each year. Fragilariopsis is a dominating psychrophilic diatom genus in polar sea ice. The two species Fragilariopsis cylindrus and Fragilariopsis curta are able to grow and divide below freezing temperature. Antifreeze proteins (AFPs), involved in cold adaptation in several psychrophilic organisms, are widespread in this two polar species. Achanthes minutissima isolated as dominant diatom has degradable effects involving petroleum hydocarbons. Phaeodactylum tricornutum, have antibacterial activity and the fatty acid, eicosapentaenoic acid (EPA), has been identified as one compound responsible for this activity. Other antibacterial compounds are monounsaturated fatty acid (9Z)-hexadecenoic acid (palmitoleic acid; C16:1 n-7) and the relatively unusual polyunsaturated fatty acid (6Z, 9Z, 12Z)-hexadecatrienoic acid (HTA; C16:3 n-4). Both are active against Gram-positive bacteria and many Gram-negative pathogen. Palmitoleic acid is active at micro-molar concentrations, kills bacteria rapidly, and is highly active against multidrug-resistant Staphylococcus aureus. Domoic acid -a neurotoxin produced by Pseudo-nitzschia accumulates in marine invertebrates. Evidences of sea lion (Zalophus californianus) and human poisoning following consumption of contaminated blue mussels (Mytilus edulis) is mainly due to this toxin. Among the most prominent features described in human beings was memory impairment which led to the name Amnesic Shellfish Poisoning [ASP]. Silicon utilizing organisms can act as a bioindicator of environmental contamination, thus a rapid change in phytochelatins to both the increase in and the withdrawal of environmental Cd stress was found in Thalassiosira nordenskioeldii. Some of them also can produce biofuels particularly diatoms have significant

  15. Modeling of GE Appliances: Final Presentation

    Energy Technology Data Exchange (ETDEWEB)

    Fuller, Jason C.; Vyakaranam, Bharat; Leistritz, Sean M.; Parker, Graham B.

    2013-01-31

    This report is the final in a series of three reports funded by U.S. Department of Energy Office of Electricity Delivery and Energy Reliability (DOE-OE) in collaboration with GE Appliances’ through a Cooperative Research and Development Agreement (CRADA) to describe the potential of GE Appliances’ DR-enabled appliances to provide benefits to the utility grid.

  16. Final Focus Test Stand final report

    CERN Document Server

    Jeremie, A; Burrows, P

    2013-01-01

    Future Linear colliders will need particle beam sizes in the nanometre range. The beam also needs to be stable all along the beam line and especially at the Final Focus section. A dedicated Final Focus test stand has been used for this study and is comprised of several sub-parts. First there is the Stabilisation/Isolation system with sensors and actuators stabilizing down to sub-nanometre level. Then the Magnet itself needs to comply with very specific design constraints. In addition to the mechanical items, the beam can be stabilized acting on the trajectory directly and Beam-based controls have been developed and tested on different accelerator facilities.

  17. Comparison of total ginkgolic acids content in leaf of fruit-utilization type of Ginkgo biloba with different tree ages and from different locations%不同产地及株龄果用银杏叶中总银杏酸含量的比较

    Institute of Scientific and Technical Information of China (English)

    姚鑫; 周桂生; 唐于平; 钱大玮; 宿树兰; 段金廒

    2012-01-01

    Total ginkgolic acids content in leaf of fruit-utilization type of Ginkgo biloba L.from 17 locations in 12 provinces and with different tree ages from 10 to 100 a were analyzed by HPLC.The results show that total ginkgolic acids content in G.biloba leaf varies with changing of locations and tree ages.Total ginkgolic acids content in 10-30 year-old G.biloba is all lower (mass ratio 0.81% -0.93% ) , in which that in 25 year-old G.biloba is the lowest ( mass ratio 0.81% ), but that in 50 and 100 year-old G.biloba is higher with a mass ratio of 1.20% and 1.07% , respectively.Total ginkgolic acids content in G.biloba leaf from Changting of Fujian is the highest (mass ratio 2.80% ) , and that from Jiangsu, Shandong, Anhui and Zhejiang is all lower (mass ratio 0.51%-0.99% ) , showing a certain regional distribution characteristics.

  18. Study on information utility

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Information has two aspects. One aspect is the objective one; another aspect is the subjective one. Shannon has discussed the objective aspect of information in information theory. But the subjective aspect of information has not been fully discussed. Someone use "Bayesian approaches" to evaluate the value of information. But in some cases it does not meet the information user's need. This paper is focus on the subjective aspect of objectively measurable information and gives formal definitions for information, information utility, and marginal information utility, normalized calculation of information utility. The information discussed in the paper has interdisciplinary nature. This work can be the foundation of many application areas.

  19. Cassini's Grand Finale: The Final Orbits

    Science.gov (United States)

    Spilker, Linda; Edgington, Scott

    2016-04-01

    The Cassini-Huygens mission, a joint collaboration between NASA, ESA and the Italian Space Agency, is approaching its last year of operations after nearly 12 years in orbit around Saturn. Cassini will send back its final bits of unique data on September 15th, 2017 as it plunges into Saturn's atmosphere, vaporizing and satisfying planetary protection requirements. Before that time Cassini will continue its legacy of exploration and discovery with 12 close flybys of Titan in 2016 and 2017 that will return new science data as well as sculpt the inclinations and periods of the final orbits. Even though all of our close icy satellite flybys, including those of Enceladus, are now completed, numerous Voyager-class flybys (summer solstice approaches. In November 2016 Cassini will transition to a series of orbits with peripases just outside Saturn's F ring. These 20 orbits will include close flybys of some tiny ring moons and excellent views of the F ring and outer A ring. The 126th and final close flyby of Titan will propel Cassini across Saturn's main rings and into its final orbits. Cassini's Grand Finale, starting in April 2017, is comprised of 22 orbits at an inclination of 63 degrees. Cassini will repeatedly dive between the innermost rings and the upper atmosphere of the planet providing insights into fundamental questions unattainable during the rest of the mission. Cassini will be the first spacecraft to explore this region. These close orbits provide the highest resolution observations of both the rings and Saturn, and direct in situ sampling of the ring particles, composition, plasma, Saturn's exosphere and the innermost radiation belts. Saturn's gravitational field will be measured to unprecedented accuracy, providing information on the interior structure of the planet, winds in the outer layers of Saturn's atmosphere, and the mass distribution in the rings. Probing the magnetic field will give insight into the nature of the magnetic dynamo, telling us: why the

  20. {sup 26}Al-containing acidic and basic sodium aluminum phosphate preparation and use in studies of oral aluminum bioavailability from foods utilizing {sup 26}Al as an aluminum tracer

    Energy Technology Data Exchange (ETDEWEB)

    Yokel, Robert A. [College of Pharmacy, 511C Pharmacy Building 725 Rose Street, University of Kentucky Medical Center, Lexington, KY 40536-0082 (United States) and Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536-0305 (United States)]. E-mail: ryokel@email.uky.edu; Urbas, Aaron A. [Department of Chemistry, University of Kentucky, Lexington, KY 40506-0055 (United States); Lodder, Robert A. [College of Pharmacy, 511C Pharmacy Building 725 Rose Street, University of Kentucky Medical Center, Lexington, KY 40536-0082 (United States); Department of Chemistry, University of Kentucky, Lexington, KY 40506-0055 (United States); Selegue, John P. [Department of Chemistry, University of Kentucky, Lexington, KY 40506-0055 (United States); Florence, Rebecca L. [College of Pharmacy, 511C Pharmacy Building 725 Rose Street, University of Kentucky Medical Center, Lexington, KY 40536-0082 (United States)

    2005-04-01

    We synthesized {sup 26}Al-containing acidic and basic (alkaline) sodium aluminum phosphates (SALPs) which are FDA-approved leavening and emulsifying agents, respectively, and used them to determine the oral bioavailability of aluminum incorporated in selected foods. We selected applicable methods from published syntheses (patents) and scaled them down ({approx}3000- and 850-fold) to prepare {approx}300-400 mg of each SALP. The {sup 26}Al was incorporated at the beginning of the syntheses to maximize {sup 26}Al and {sup 27}Al equilibration and incorporate the {sup 26}Al in the naturally-occurring Al-containing chemical species of the products. Near infrared spectroscopy (NIR) and X-ray powder diffraction (XRD) were used to characterize the two SALP samples and some intermediate samples. Multi-elemental analysis (MEA) was used to determine Na, Al and P content. Commercial products were included for comparison. Satisfactory XRD analyses, near infrared spectra and MEA results confirmed that we synthesized acidic and basic SALP, as well as some of the syntheses intermediates. The {sup 26}Al-containing acidic and basic SALPs were incorporated into a biscuit material and a processed cheese, respectively. These were used in oral bioavailability studies conducted in rats in which the {sup 26}Al present in blood after its oral absorption was quantified by accelerator mass spectrometry. The results showed oral Al bioavailability from acidic SALP in biscuit was {approx}0.02% and from basic SALP in cheese {approx}0.05%, lower than our previous determination of Al bioavailability from drinking water, {approx}0.3%. Both food and water can appreciably contribute to the Al absorbed from typical human Al intake.

  1. Utility of Genetic Testing for Confirmation of Abnormal Newborn Screening in Disorders of Long-Chain Fatty Acids: A Missed Case of Carnitine Palmitoyltransferase 1A (CPT1A) Deficiency

    OpenAIRE

    Dowsett, Leah; Lulis, Lauren; Ficicioglu, Can; Cuddapah, Sanmati

    2017-01-01

    An 18-month-old male was evaluated after presenting with disproportionately elevated liver transaminases in the setting of acute gastroenteritis. He had marked hepatomegaly on physical exam that was later confirmed with an abdominal ultrasound. Given this clinical picture, suspicion for a fatty acid oxidation disorder was raised. Further investigation revealed that his initial newborn screen was positive for carnitine palmitoyltransferase 1A (CPT1A) deficiency?a rare autosomal recessive disor...

  2. Information, Utility & Bounded Rationality

    CERN Document Server

    Ortega, Pedro A

    2011-01-01

    Perfectly rational decision-makers maximize expected utility, but crucially ignore the resource costs incurred when determining optimal actions. Here we employ an axiomatic framework for bounded rational decision-making based on a thermodynamic interpretation of resource costs as information costs. This leads to a variational "free utility" principle akin to thermodynamical free energy that trades off utility and information costs. We show that bounded optimal control solutions can be derived from this variational principle, which leads in general to stochastic policies. Furthermore, we show that risk-sensitive and robust (minimax) control schemes fall out naturally from this framework if the environment is considered as a bounded rational and perfectly rational opponent, respectively. When resource costs are ignored, the maximum expected utility principle is recovered.

  3. Chemical Search Web Utility

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Chemical Search Web Utility is an intuitive web application that allows the public to easily find the chemical that they are interested in using, and which...

  4. Utilization of lignin

    Energy Technology Data Exchange (ETDEWEB)

    Machihara, A.

    1981-01-01

    The chemical structure of lignin, the composition of pulping spent liquors, conversion of lignin into fine chemicals, utilization of lignin products, and physical and chemical properties of lignin and and derivatives are reviewed. (Refs 29).

  5. UtilityOther_ELCFRANCHISE

    Data.gov (United States)

    Vermont Center for Geographic Information — ELCFRANCHISE includes Vermont's Electric Utility Franchise boundaries. It is a compilation of many data sources. The boundaries are approximate and should be used...

  6. UtilityTelecom_TELEFAC

    Data.gov (United States)

    Vermont Center for Geographic Information — The UtilityTelecom_TELEFAC data layer contains points which are intended to represent the location of telecommunications facilities (towers and/or antennas) in...

  7. Biotechnology of space utilization

    Energy Technology Data Exchange (ETDEWEB)

    Matsumiya, Hiroyuki

    1989-10-05

    This paper introdued the practical results of biotechnological experiments utilizing features of space and the present Japanese plan. The electrophoresis and crystal growth of protein utilize the microgravity field and cell culture utilizes the influence of microgravity on organism. Of practical results in space experiments, the electrophoresis was made by US but others were carried out by Europian countries. It was thought that the electrophoresis utilizing space environment could be demonstrated to be promosing for the separating and refining techniques by the experiment on the basis of the Apolo Project. The crystal growth of protein was reported by Litke of Denmark as to the crystal of lyzozyme. The space experiment of cell culture was carried out by Cogoli of Switzerland as to the culture of lymphocyte in the Shattle. Japan is studying the primary material experiment project(FMPT) in the Space Shattle and unmanned experiment for electrophoresis. 9 refs., 3 tabs.

  8. MSIS Drug Utilization Datamart

    Data.gov (United States)

    U.S. Department of Health & Human Services — This page provides background needed to take advantage of the capabilities of the MSIS Drug Utilization Datamart. This mart allows the user to develop high-level...

  9. Utility requirements for fusion

    Energy Technology Data Exchange (ETDEWEB)

    Vondrasek, R.J.

    1982-02-01

    This report describes work done and results obtained during performance of Task 1 of a study of Utility Requirements and Criteria for Fusion Options. The work consisted of developing a list of utility requirements for fusion optics containing definition of the requirements and showing their relative importance to the utility industry. The project team members developed a preliminary list which was refined by discussions and literature searches. The refined list was recast as a questionnaire which was sent to a substantial portion of the utility industry in this country. Forty-three questionnaire recipients responded including thirty-two utilities. A workshop was held to develop a revised requirements list using the survey responses as a major input. The list prepared by the workshop was further refined by a panel consisting of vice presidents of the three project team firms. The results of the study indicate that in addition to considering the cost of energy for a power plant, utilities consider twenty-three other requirements. Four of the requirements were judged to be vital to plant acceptability: Plant Capital Cost, Financial Liability, Plant Safety and Licensability.

  10. A general classification of silicon utilizing organisms

    Science.gov (United States)

    Das, P.; Das, S.

    2010-12-01

    Silicon utilizing organisms may be defined as organisms with high silicon content (≥ 1% dry weight) and they can metabolize silicon with or without demonstrable silicon transporter genes (SIT) in them(Das,2010). Silicon is the second most abundant element in the lithosphere (27.70%) and it is as important as phosphorus and magnesium (0.03%) in the biota. Hydrated silica represents the second most abundant biogenic mineral after carbonate minerals. Silicon is accumulated and metabolized by some prokaryotes, and Si compounds can stimulate the growth of a range of fungi. It is well known that Si is essential for diatoms. In mammals, Si is considered an essential trace element, required in bone, cartilage and connective tissue formation, enzymatic activities and other metabolic processes. Silicon was suggested to act as a phosphoprotein effector in bone. In mammals, Si is also reported to positively influence the immune system and to be required for lymphocyte proliferation. The aqueous chemistry of Si is dominated by silicic acid at biological pH ranges. Monosilicic acid can form stable complexes with or