Nef’î Hakkında Bir Bibliyografya Denemesi An Essay Of Bibliography About Nef’i

Directory of Open Access Journals (Sweden)

Halil Sercan KOŞİK

2013-03-01

Full Text Available Nef’î who lived in the first half of 17th century was born in Hasankale one of districts of Erzurum. The poet whose real name was Ömer had a life full of struggles but he managed to produce some important works through his life. Nef’î, one of the leading poets of divan poetry was celebrated much at his age and after his death. Such a reputation is originated from the sharpness of his pen, his talent as well as his wit. Nef’î whose usage of praise and satire is extreme is known forhis mastership in using rhythm and rhyme in his poems. While attaching importance to meaning and utterance he tried to use a new language and accomplished that to a great degree. His knowledge of Persian Language and Literature played a considerable role.A lot has been written so far about Nef’î and a great many research exists about his works too. However it is obvious that there will be many works about Nef’î, a productive poet. In this study a classification of Nef’î’s works and scientific studies about him and his works will be presented. Our research focuses to illustrate which part of his works is the center of modern researches and which parts are not. In addition, it is expected that our research will provided useful information to researchers studying Nef’î as a guide for sources regarding him. On yedinci yüzyılın ilk yarısında yaşamış olan Nef’î, Erzurum’un Hasankale ilçesinde doğmuştur. Asıl adı Ömer olan şair, oldukça mücadele dolu bir hayat geçirmiş ve bu hayata aynı zamanda önemli denilebilecek eserler de sığdırabilmeyi başarmıştır. Divan şiirinin önde gelen şairlerden biri olan Nef’î, adından hem yaşadığı dönemde hem de sonrasında çokça söz ettirmiştir. Nef’î’nin böyle bir şöhrete kavuşması onun edebi yeteneği ve kıvrak zekası yanında kaleminin keskin olmasından kaynaklanmaktadır. Övdüğünü çok iyi öven, yerdiğini de çok kötü bir şekilde yeren Nef�

[Effect of Recombinant Adenovirus AdE-SH2-Caspase 8 on the Apoptosis of Imatinib-resistant K562/G01 Cell Line].

Science.gov (United States)

Wang, Lin; Fei, Chang; Huang, Zheng-Lan; Li, Hui; Liu, Zhang-Lin; Feng, Wen-Li

2015-08-01

To investigate the effect of SH2-Caspase 8 fusion protein expressed by recombinant adenovirus AdE-SH2-Caspase8-HA-GFP (SC) on the apoptosis of K562/G01 cell line, which is a BCR/ABL positive chronic myeloid leukemia cell line and resistant to imatinib. The K562/G01 cell line was infected with AdE-SH2-Caspase 8-HA-GFP adenovirus (SC), then the cells were divided into 3 groups: AdE-SH2m-Caspase 8-HA-GFP (SmC) group, AdE-GFP (CMV) group and PBS group as control. The infection efficiency was observed under fluorescent microscopy and by flow cytometry. The expression of fusion protein SH2-Caspase 8-HA was measured by Western blot. The morphology of the cells detected by Wright's staining. The apoptosis of the cells were detected by flow cytometry and DNA ladder. The expression of Caspase 3 and PARP were detected by Western blot. The infection efficiency of SC on K562/G01 cells was high which was confirmed by fluorescent microscopy and FCM. SH2-Caspase 8-HA fusion protein were expressed correctly in K562/G01 cells. After treatment with SC the apoptosis of K562/G01 cells could be observed by microscopy. The result of FCM showed that early apoptosis of K562/G01 cells increased significantly as compared with control groups (P SH2-Caspase 8 fusion protein can induces the apoptosis of K562/G01 cells.

Clusters of basic amino acids contribute to RNA binding and nucleolar localization of ribosomal protein L22.

Directory of Open Access Journals (Sweden)

Jennifer L Houmani

Full Text Available The ribosomal protein L22 is a component of the 60S eukaryotic ribosomal subunit. As an RNA-binding protein, it has been shown to interact with both cellular and viral RNAs including 28S rRNA and the Epstein-Barr virus encoded RNA, EBER-1. L22 is localized to the cell nucleus where it accumulates in nucleoli. Although previous studies demonstrated that a specific amino acid sequence is required for nucleolar localization, the RNA-binding domain has not been identified. Here, we investigated the hypothesis that the nucleolar accumulation of L22 is linked to its ability to bind RNA. To address this hypothesis, mutated L22 proteins were generated to assess the contribution of specific amino acids to RNA binding and protein localization. Using RNA-protein binding assays, we demonstrate that basic amino acids 80-93 are required for high affinity binding of 28S rRNA and EBER-1 by L22. Fluorescence localization studies using GFP-tagged mutated L22 proteins further reveal that basic amino acids 80-93 are critical for nucleolar accumulation and for incorporation into ribosomes. Our data support the growing consensus that the nucleolar accumulation of ribosomal proteins may not be mediated by a defined localization signal, but rather by specific interaction with established nucleolar components such as rRNA.

The states of the art of the nondestructive assay of spent nuclear fuel assemblies. A critical review of the Spent Fuel NDA Project of the U.S. Department of Energy's Next Generation Safeguards Initiative

International Nuclear Information System (INIS)

Bolind, Alan Michael; Seya, Michio

2015-12-01

The state of the art of the nondestructive assay of spent nuclear fuel assemblies is represented by the results of the Spent Fuel Nondestructive Assay Project of the Next Generation Safeguards Initiative (NGSI) of the U.S. Department of Energy / National Nuclear Security Administration. This report surveys the fourteen advanced nondestructive assay (NDA) techniques that were examined by the NGSI. For each technique, it explains how the technique operates, the NGSI's design of an instrument that uses the technique, how the data are analyzed, and the technique's chief limitations. After this survey of the NDA techniques, the report then discusses and critiques the current paradigm of the practice of NDA of spent fuel assemblies. It shows how the current main problem in the NDA of spent fuel assemblies—namely, an unacceptably large uncertainty in the assay results—is caused primarily by using too few independent NDA measurements. Because the physics of the NDA of spent fuel assemblies is three dimensional, at least three independent NDA measurements are required. Thus, NDA results should be able to be improved dramatically by combining the fourteen advanced NDA techniques plus other existing NDA techniques into appropriate combinations of three techniques. This report evaluates the NGSI's proposed NDA combinations according to these principles. (author)

Corrective Action Decision Document for Corrective Action Unit 562: Waste Systems Nevada Test Site, Nevada, Revision 0

Energy Technology Data Exchange (ETDEWEB)

Mark Krause

2010-08-01

This Corrective Action Decision Document (CADD) presents information supporting the selection of corrective action alternatives (CAAs) leading to the closure of Corrective Action Unit (CAU) 562, Waste Systems, in Areas 2, 23, and 25 of the Nevada Test Site, Nevada. This complies with the requirements of the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the State of Nevada; U.S. Department of Energy (DOE), Environmental Management; U.S. Department of Defense; and DOE, Legacy Management. Corrective Action Unit 562 comprises the following corrective action sites (CASs): • 02-26-11, Lead Shot • 02-44-02, Paint Spills and French Drain • 02-59-01, Septic System • 02-60-01, Concrete Drain • 02-60-02, French Drain • 02-60-03, Steam Cleaning Drain • 02-60-04, French Drain • 02-60-05, French Drain • 02-60-06, French Drain • 02-60-07, French Drain • 23-60-01, Mud Trap Drain and Outfall • 23-99-06, Grease Trap • 25-60-04, Building 3123 Outfalls The purpose of this CADD is to identify and provide the rationale for the recommendation of CAAs for the 13 CASs within CAU 562. Corrective action investigation (CAI) activities were performed from July 27, 2009, through May 12, 2010, as set forth in the CAU 562 Corrective Action Investigation Plan. The purpose of the CAI was to fulfill the following data needs as defined during the data quality objective (DQO) process: • Determine whether COCs are present. • If COCs are present, determine their nature and extent. • Provide sufficient information and data to complete appropriate corrective actions. A data quality assessment (DQA) performed on the CAU 562 data demonstrated the quality and acceptability of the data for use in fulfilling the DQO data needs. Analytes detected during the CAI were evaluated against appropriate final action levels (FALs) to identify the COCs for each CAS. The results of the CAI identified COCs at 10 of the 13 CASs in CAU 562, and thus corrective

Use of zinc-finger nucleases to knock out the WAS gene in K562 cells: a human cellular model for Wiskott-Aldrich syndrome

Directory of Open Access Journals (Sweden)

Miguel G. Toscano

2013-03-01

Mutations in the WAS gene cause Wiskott-Aldrich syndrome (WAS, which is characterized by eczema, immunodeficiency and microthrombocytopenia. Although the role of WASP in lymphocytes and myeloid cells is well characterized, its role on megakaryocyte (MK development is poorly understood. In order to develop a human cellular model that mimics the megakaryocytic-derived defects observed in WAS patients we used K562 cells, a well-known model for study of megakaryocytic development. We knocked out the WAS gene in K562 cells using a zinc-finger nuclease (ZFN pair targeting the WAS intron 1 and a homologous donor DNA that disrupted WASP expression. Knockout of WASP on K562 cells (K562WASKO cells resulted in several megakaryocytic-related defects such as morphological alterations, lower expression of CD41ɑ, lower increments in F-actin polymerization upon stimulation, reduced CD43 expression and increased phosphatidylserine exposure. All these defects have been previously described either in WAS-knockout mice or in WAS patients, validating K562WASKO as a cell model for WAS. However, K562WASPKO cells showed also increased basal F-actin and adhesion, increased expression of CD61 and reduced expression of TGFβ and Factor VIII, defects that have never been described before for WAS-deficient cells. Interestingly, these phenotypic alterations correlate with different roles for WASP in megakaryocytic differentiation. All phenotypic alterations observed in K562WASKO cells were alleviated upon expression of WAS following lentiviral transduction, confirming the role of WASP in these phenotypes. In summary, in this work we have validated a human cellular model, K562WASPKO, that mimics the megakaryocytic-related defects found in WAS-knockout mice and have found evidences for a role of WASP as regulator of megakaryocytic differentiation. We propose the use of K562WASPKO cells as a tool to study the molecular mechanisms involved in the megakaryocytic-related defects observed in WAS

Chaetominine reduces MRP1-mediated drug resistance via inhibiting PI3K/Akt/Nrf2 signaling pathway in K562/Adr human leukemia cells

Energy Technology Data Exchange (ETDEWEB)

Yao, Jingyun; Wei, Xing [State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai (China); Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai (China); Lu, Yanhua, E-mail: luyanhua@ecust.edu.cn [State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai (China); Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai (China)

2016-05-13

Drug resistance limits leukemia treatment and chaetominine, a cytotoxic alkaloid that promotes apoptosis in a K562 human leukemia cell line via the mitochondrial pathway was studied with respect to chemoresistance in a K562/Adr human resistant leukemia cell line. Cytotoxicity assays indicated that K562/Adr resistance to adriamycin (ADR) did not occur in the presence of chaetominine and that chaetominine increased chemosensitivity of K562/Adr to ADR. Data show that chaetominine enhanced ADR-induced apoptosis and intracellular ADR accumulation in K562/Adr cells. Accordingly, chaetominine induced apoptosis by upregulating ROS, pro-apoptotic Bax and downregulating anti-apoptotic Bcl-2. RT-PCR and western-blot confirmed that chaetominine suppressed highly expressed MRP1 at mRNA and protein levels. But little obvious alternation of another drug transporter MDR1 mRNA was observed. Furthermore, inhibition of MRP1 by chaetominine relied on inhibiting Akt phosphorylation and nuclear Nrf2. In summary, chaetominine strongly reverses drug resistance by interfering with the PI3K/Akt/Nrf2 signaling, resulting in reduction of MRP1-mediated drug efflux and induction of Bax/Bcl-2-dependent apoptosis in an ADR-resistant K562/Adr leukemia cell line. - Highlights: • Chaetominine enhanced chemosensitivity of ADR against K562/Adr cells. • Chaetominine increased intracellular ADR levels via inhibiting MRP1. • Chaetominine induced apoptosis of K562/Adr cells through upregulation of ROS and modulation of Bax/Bcl-2. • Inhibition of MRP1 and Nrf2 by chaetominine treatment was correlative with blockade of PI3K/Akt signaling.

How the NDA Provides Transparency and Visibility of the Technical Deliverability of the R and D Programme - 13303

International Nuclear Information System (INIS)

Seed, Ian; James, Paula; Brownridge, Melanie; McMinn, Mervin

2013-01-01

The Nuclear Decommissioning Authority (NDA) was created under the UK Energy Act 2004 to ensure the UK historic civil public sector nuclear legacy sites are decommissioned safely, securely, cost effectively and in ways that protect the environment. The delivery will involve carrying out many unique projects within a high hazard environment requiring the very highest standards in safety, security and environmental management. Unique problems require unique solutions and there is a substantial amount of research and development required for each project. The NDA's R and D strategic objective is to ensure that delivery of the NDA's mission is technically underpinned by sufficient and appropriate research and development. This drives a requirement to provide transparency and visibility of the technical deliverability of the programme through the technical baseline and accompanying research and development requirements. The NDA need to have confidence in the technical deliverability of the Site License Companies (SLCs) plans, provide overall visibility of R and D across the NDA Estate and ensure that appropriate R and D is being carried out in a timely manner. They need to identify where coordinated R and D programmes may be advantageous as a result of common needs, risks and opportunities and ensure key R and D needs across NDA are identified, prioritised and work programmes are costed and scheduled in the Lifetime Plans for individual sites and SLCs. Evidence of the Site License Company's approach and their corresponding technical underpinning programmes is achieved through submission of a number of outputs collectively known as TBuRDs (Technical Baseline and Underpinning Research and Development Requirements). This paper is a summary of the information generated by an independent review of those TBuRDs. It highlights some of the key messages, synergies and common R and D activities across the estate. It demonstrates the value of a consistent approach to collecting R

Anticancer activity of Pupalia lappacea on chronic myeloid leukemia K562 cells.

Science.gov (United States)

Ravi, Alvala; Alvala, Mallika; Sama, Venkatesh; Kalle, Arunasree M; Irlapati, Vamshi K; Reddy, B Madhava

2012-12-05

Cancer is one of the most prominent human diseases which has enthused scientific and commercial interest in the discovery of newer anticancer agents from natural sources. Here we demonstrated the anticancer activity of ethanolic extract of aerial parts of Pupalia lappacea (L) Juss (Amaranthaceae) (EAPL) on Chronic Myeloid Leukemia K562 cells. Antiproliferative activity of EAPL was determined by MTT assay using carvacrol as a positive control. Induction of apoptosis was studied by annexin V, mitochondrial membrane potential, caspase activation and cell cycle analysis using flow cytometer and modulation in protein levels of p53, PCNA, Bax and Bcl2 ratio, cytochrome c and cleavage of PARP were studied by Western blot analysis. The standardization of the extract was performed through reverse phase-HPLC using Rutin as biomarker. The results showed dose dependent decrease in growth of K562 cells with an IC50 of 40 ± 0.01 μg/ml by EAPL. Induction of apoptosis by EAPL was dose dependent with the activation of p53, inhibition of PCNA, decrease in Bcl2/Bax ratio, decrease in the mitochondrial membrane potential resulting in release of cytochrome c, activation of multicaspase and cleavage of PARP. Further HPLC standardization of EAPL showed presence 0.024% of Rutin. Present study significantly demonstrates anticancer activity of EAPL on Chronic Myeloid Leukemia (K562) cells which can lead to potential therapeutic agent in treating cancer. Rutin, a known anti cancer compound is being reported and quantified for the first time from EAPL.

Methodology for NDA performance assessment

International Nuclear Information System (INIS)

Cuypers, M.; Franklin, M.; Guardini, S.

1986-01-01

In the framework of the RandD programme of the Joint Research Centre of the Commission of the European Communities, a considerable effort is being dedicated to performance assessment of NDA techniques taking account of field conditions. By taking account of field conditions is meant measurement samples of the size encountered in practice and training which allows inspectors to design cost efficient verification plans for the real situations encountered in the field. Special laboratory facilities referred to as PERLA are being constructed for this purpose. These facilities will be used for measurement experiments and for training. In this paper, performance assessment is discussed under the headings of measurement capability and in-field effectiveness. Considerable emphasis is given to the role of method specific measurement error models. The authors outline the advantages of giving statistical error models a sounder basis in the physical phenomenology of the measurement method

Peynir Yapımında Mikrobiyal Renet Kullanımı

Directory of Open Access Journals (Sweden)

Mehmet Karapınar

2015-02-01

Full Text Available Peynir yapımında süt koagulantı olarak buzağı reneti yerine kullanılmak üzere proteazlar üzerine yapılan çalışmalar son yıllarda yoğunluk kazanmıştır. Günümüzde buzağı reneti standart bir süt koagulantı olarak önemini sürdürmekteyse de, şirden mayası üretimi her yıl giderek gereksinimi karşılayamaz duruma gelmektedir. Bunun sonucu olarak yeni renet kaynakları arasında yoğunlaşmış ve günümüzde Mucor pusillus, M. miehei, Endothia parasitica küf türlerinden elde edilen üç fungal enzim ile Bacillus cereus bakterisinden elde edilen bir bakteriyel enzim ticari olarak üretilmeye başlanmıştır.

NDA issues with RFETS vitrified waste forms

International Nuclear Information System (INIS)

Hurd, J.; Veazey, G.

1998-01-01

A study was conducted at Los Alamos National Laboratory (LANL) for the purpose of determining the feasibility of using a segmented gamma scanner (SGS) to accurately perform non-destructive analysis (NDA) on certain Rocky Flats Environmental Technology Site (RFETS) vitrified waste samples. This study was performed on a full-scale vitrified ash sample prepared at LANL according to a procedure similar to that anticipated to be used at RFETS. This sample was composed of a borosilicate-based glass frit, blended with ash to produce a Pu content of ∼1 wt %. The glass frit was taken to a degree of melting necessary to achieve a full encapsulation of the ash material. The NDA study performed on this sample showed that SGSs with either 1/2- or 2-inch collimation can achieve an accuracy better than 6 % relative to calorimetry and γ-ray isotopics. This accuracy is achievable, after application of appropriate bias corrections, for transmissions of about 1/2 % through the waste form and counting times of less than 30 minutes. These results are valid for ash material and graphite fines with the same degree of plutonium particle size, homogeneity, sample density, and sample geometry as the waste form used to obtain the results in this study. A drum-sized thermal neutron counter (TNC) was also included in the study to provide an alternative in the event the SGS failed to meet the required level of accuracy. The preliminary indications are that this method will also achieve the required accuracy with counting times of ∼30 minutes and appropriate application of bias corrections. The bias corrections can be avoided in all cases if the instruments are calibrated on standards matching the items

Developing Spent Fuel Assembly for Advanced NDA Instrument Calibration - NGSI Spent Fuel Project

Energy Technology Data Exchange (ETDEWEB)

Hu, Jianwei [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Gauld, Ian C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Banfield, James [GE Hitachi Nuclear Energy, Wilmington, NC (United States); Skutnik, Steven [Univ. of Tennessee, Knoxville, TN (United States)

2014-02-01

This report summarizes the work by Oak Ridge National Laboratory to investigate the application of modeling and simulation to support the performance assessment and calibration of the advanced nondestructive assay (NDA) instruments developed under the Next Generation Safeguards Initiative Spent Fuel (NGSI-SF) Project. Advanced NDA instrument calibration will likely require reference spent fuel assemblies with well-characterized nuclide compositions that can serve as working standards. Because no reference spent fuel standard currently exists, and the practical ability to obtain direct measurement of nuclide compositions using destructive assay (DA) measurements of an entire fuel assembly is prohibitive in the near term due to the complexity and cost of spent fuel experiments, modeling and simulation will be required to construct such reference fuel assemblies. These calculations will be used to support instrument field tests at the Swedish Interim Storage Facility (Clab) for Spent Nuclear Fuel.

Eskişehir koşullarında yetiştirilen Reyhan (Ocimum basilicum L.) bitkisinin bazı bitkisel özelliklerin ve diurnal varyabilitesinin belirlenmesi

OpenAIRE

Kulan, Engin Gökhan

2013-01-01

Bu çalışma Eskişehir Osmangazi Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü deneme alanında 2010 yılında yürütülmüştür. Denemede Eskişehir ekolojik koşullarında yetiştirilen reyhan (Ocimum basilicum L.) bitkisinin verim ve verim özellikleri ile farklı biçim saatlerinin reyhanın uçucu yağı ve uçucu yağ bileşimi üzerine olan etkileri incelenmiştir. Tesadüf bloklarında bölünmüş parseller deneme desenine göre 3 tekerrürlü olarak kurulan denemede reyhan bitkileri çiçeklen...

NDA for a new facility at SRP

International Nuclear Information System (INIS)

Studley, R.V.

1987-01-01

In 1980, plans were initiated to build a new facility to process high purity highly enriched 235 U. Interest in improving nuclear safeguards had been increasing in the previous few years. A basic objective of the design, therefore, included achievement of maximum performance in special nuclear material (SNM) accountability. A near-real-time accountability system with high accuracy assay measurements was developed for this purpose. Simultaneous design of facility, process equipment, and the accountability system allowed maximum integration of equipment and also permitted influence on process design and material characteristics to optimize accountability performance. This was an ideal situation in which to pursue maximum improvement in nondestructive assay (NDA) measurement performance. The resulting systems are described

Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway

Energy Technology Data Exchange (ETDEWEB)

Zhang, Huijuan; Tan, Shi; Wang, Juan; Chen, Shana; Quan, Jing; Xian, Jingrong; Zhang, Shuai shuai; He, Jingang; Zhang, Ling, E-mail: lingzhang@cqmu.edu.cn

2014-01-01

The RNA-binding protein Musashi2 (Msi2) has been identified as a master regulator within a variety of stem cell populations via the regulation of translational gene expression. A recent study has suggested that Msi2 is strongly expressed in leukemic cells of acute myeloid leukemia patients, and elevated Msi2 is associated with poor prognosis. However, the potential role of Msi2 in leukemogenesis is still not well understood. Here, we investigated the effect of Msi2 knockdown on the biological properties of leukemic cells. High expression of Msi2 was found in K562 and KG-1a leukemic cell lines, and low expression was observed in the U937 cell line. We transduced K562 cells with two independent adenoviral shRNA vectors targeting Msi2 and confirmed knockdown of Msi2 at the mRNA and protein levels. Msi2 silencing inhibited cell growth and caused cell cycle arrest by increasing the expression of p21 and decreasing the expression of cyclin D1 and cdk2. In addition, knockdown of Msi2 promoted cellular apoptosis via the upregulation of Bax and downregulation of Bcl-2 expression. Furthermore, Msi2 knockdown resulted in the inactivation of the ERK/MAPK and p38/MAPK pathways, but no remarkable change in p-AKT was observed. These data provide evidence that Msi2 plays an important role in leukemogenesis involving the MAPK signaling pathway, which indicates that Msi2 may be a novel target for leukemia treatment. - Highlights: • Knockdown of Msi2 inhibited K562 cell growth and arrested cell cycle progression. • Knockdown of Msi2 induced K562 cell apoptosis via the regulation of Bax and Bcl-2. • The MAPK pathway was involved in the process of Msi2-mediated leukemogenesis. • Our data indicate that Msi2 is a potential new target for leukemia treatment.

The proteomic study of sodium butyrate antiproliferative/cytodifferentiation effects on K562 cells

Czech Academy of Sciences Publication Activity Database

Grebeňová, D.; Kuželová, K.; Pluskalová, M.; Pešlová, G.; Halada, Petr; Hrkal, Z.

2006-01-01

Roč. 37, - (2006), s. 210-217 ISSN 1079-9796 R&D Projects: GA MZd NL7681 Institutional research plan: CEZ:AV0Z50200510 Keywords : k562 * cell cycle * hemoglobin synthesis Subject RIV: EE - Microbiology, Virology Impact factor: 2.678, year: 2006

Production of NDA Working Reference Materials for the Capability Evaluation Project

International Nuclear Information System (INIS)

Noll, P.D. Jr.; Marshall, R.S.

1998-01-01

The production of Non Destructive Assay (NDA) Working Reference Materials (WRMs) that are traceable to nationally recognized standards was undertaken to support implementation of the Idaho National Engineering and Environmental Laboratory (INEEL) Nondestructive Waste Assay Capability Evaluation Project (CEP). The WRMs produced for the CEP project consist of Increased Am/Pu mass ration (IAP) and depleted Uranium (DU) WRMs. The CEP IAP/DU WRM set provides radioactive material standards for use in combination with 55 gallon drum waste matrix surrogates for the assessment of waste NDA assay system performance. The Production of WRMs is a meticulous process that is not without certain trials and tribulations. Problems may arise at any of the various stages of WRM production which include, but are not limited to; material characterization (physical, chemical, and isotopic), material blend parameters, personnel radiation exposure, gas generation phenomenon, traceability to national standards, encapsulation, statistical evaluation of the data, and others. Presented here is an overall description of the process by which the CEP WRMs were produced and certified as well as discussions pertaining to some of the problems encountered and how they were solved

Roma Hukuku’nda Gemi, Han ve Ahır İşletenlerin Receptum Sorumluluğu

OpenAIRE

YEŞİLLER, Mehmet

2013-01-01

Çalışmamızda Roma Hukuku'nda gemi, han ve ahır işleten kimselerin sorumluluklarına ilişkin düzenlemeler ele alınmıştır. Roma?da özellikle gemi, han ve ahır işleten kimselerin yanlarında çalıştırdıkları kişilerin güvenilir olmamasından dolayı, taraflar arasındaki istisna sözleşmesinden kaynaklanan custodia sorumluluğuna ek olarak "actio de damno aut furto adversus nautas, caupones, stabularios ve receptum, nautae, cauponis, stabularii" sorumluluklarının düzenlendiği kaynaklardan...

Menemen Ekolojik Koşullarında Lavanta (Lavandula spp.) Tür ve Çeşitlerinin Morfolojik, Verim ve Kalite Özelliklerinin Belirlenmesi

OpenAIRE

Karık, Ünal; Çiçek, Fatih; Çınar, Orçun

2017-01-01

Bu çalışma Menemen ekolojik koşullarında lavanta (Lavandula spp.) tür ve çeşitlerininmorfolojik, verim ve kalite özelliklerini belirlemek amacı ile 2015-2016yılları arasında yürütülmüştür. Çalışmada 2 farklı türe ait 8 farklı ticarilavanta çeşidi kullanılmıştır. 2 yıl sonucunda elde edilen veriler ışığında türve çeşitler arasında önemli farklılıklar bulunduğu tespit edilmiştir. Lavandintipi çeşitler, verim özellikleri açısından lavander tipi çeşitlerin önünegeçerken,  uçucu yağ oranı ve uçucu...

Dilbilim Kavramları Işığında Türkçe Öğretimi

Directory of Open Access Journals (Sweden)

Nilay Çağlayan DİLBER

2017-12-01

Full Text Available Anadili olarak Türkçe öğretiminde hem öğretmen yetiştirme hem de ilköğretim öğrencisi yetiştirme anlamında dilbilime ve dilbilimin elde ettiği verilere yeterince önem verilmemektedir. Oysa gelişmiş ülkelerdeki anadili eğitimine bakıldığında dilbilimden ve verilerinden sıkça yararlanılmaktadır. Yapılan çalışmada da bu eksikliği gidermek adına dilbilim kuramlarında yer alan kavramların anadili olarak Türkçe öğretiminde somut olarak nasıl yer alabileceğini ve zaten yer aldığını göstermek amaçlanmaktadır. Bu doğrultuda F. De Saussure ile bir bilim haline gelen ve 19. yy. itibariyle kuramlar üzerine yeni kuramlar üreterek dili farklı açılardan betimleyen Genel Dilbilim ders notları, Avrupa Yapısalcılığı, Amerikan Yapısalcılığı, Üretici Dönüşümsel Dilbilgisi, Edimbilim ve Sözceleme Kuramları’nda kullanılan ve bu kuramlarla ortaya çıkan bazı kavramların Türkçe öğretiminde kullanımı somut bir şekilde örneklendirilerek verilmiştir. Bu örneklerin Türkçe ders kitaplarına, etkinliklere, öğretim materyallerine yardımcı olacağı öngörülmektedir. Ayrıca çalışmanın Türkçe öğretmen adaylarının dilbilime daha somut bir şekilde yaklaşmasına ve dilbilimin dil öğretimindeki gerekliliğini anlamalarına yardımcı olacağı düşünülmektedir.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on Dihydrocapsiate

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on the safety of a synthetic dihydrocapsiate (DHC) as a food ingredient in the context of Regulation (EC) No 258/97 taking into account...

Yeni Ürün Geliştirme Takımlarında Güven ve Takım Öğrenmesi

Directory of Open Access Journals (Sweden)

Volkan POLAT

2018-01-01

Full Text Available Farklı örgütsel seviyelerde güven ve öğrenme üzerine yapılan çalışmaların yanı sıra, güvenin takım bağlamında bilgi paylaşımı ve edinimi için önemli bir faktör olduğunu bildiren birtakım çalışmalar bulunmaktadır. Ancak bugüne kadar yeni ürün geliştirme takımlarında (new product development – NPD Takım Güveni ve Takım Öğrenmesi ilişkileri hakkında çok az tartışma yapılmıştır. Bu çalışma, Takım Güveninin, çeşitli takım faktörleri (Takım Üyesi Deneyimi, Takım İstikrarı ve Takım Otonomisi ve Takım Öğrenimi arasında arabuluculuk etkisi olup olmadığını incelemeyi amaçlamaktadır.

Aqueous extract of Crataegus azarolus protects against DNA damage in human lymphoblast Cell K562 and enhances antioxidant activity.

Science.gov (United States)

Mustapha, Nadia; Bouhlel, Inès; Chaabane, Fadwa; Bzéouich, Imèn Mokdad; Ghedira, Kamel; Hennebelle, Thierry; Chekir-Ghedira, Leila

2014-02-01

The present study was carried out to characterize the cellular antioxidant effect of the aqueous extract of Crataegus azarolus and its antigenotoxic potential using human myelogenous cells, K562. The antioxidant capacity of this extract was evaluated by determining its cellular antioxidant activity (CAA) in K562 cells. Also, preceding antigenotoxicity assessment, its eventual genotoxicity property was investigated by evaluating its capacity to induce the DNA degradation of treated cell nuclei. As no genotoxicity was detected at different exposure times, its ability to protect cell DNA against H2O2 oxidative effect was investigated, using the "comet assay." It appears that 800 μg/mL of extract inhibited the genotoxicity induced by H2O2 with a rate of 41.30 %, after 4 h of incubation. In addition, this extract revealed a significant cellular antioxidant capacity against the reactive oxygen species in K562 cells.

Unravelling pathways downstream Sox6 induction in K562 erythroid cells by proteomic analysis

KAUST Repository

Barbarani, Gloria; Ronchi, Antonella; Ruoppolo, Margherita; Santorelli, Lucia; Steinfelder, Robert; Elangovan, Sudharshan; Fugazza, Cristina; Caterino, Marianna

2017-01-01

are accompanied with a reduced survival of Sox6-/- red blood cells, resulting in a compensated anemia. Sox6-overexpression in K562 cells and in human primary ex vivo erythroid cultures enhances erythroid differentiation and leads to hemoglobinization, the hallmark

Effect of Organic Acid Additions on the General and Localized Corrosion Susceptibility of Alloy 22 in Chloride Solutions

Energy Technology Data Exchange (ETDEWEB)

Carranza, R M; Giordano, C M; Rodr?guez, M A; Ilevbare, G O; Rebak, R B

2007-08-28

Electrochemical studies such as cyclic potentiodynamic polarization (CPP) and electrochemical impedance spectroscopy (EIS) were performed to determine the corrosion behavior of Alloy 22 (N06022) in 1M NaCl solutions at various pH values from acidic to neutral at 90 C. All the tested material was wrought Mill Annealed (MA). Tests were also performed in NaCl solutions containing weak organic acids such as oxalic, acetic, citric and picric. Results show that the corrosion rate of Alloy 22 was significantly higher in solutions containing oxalic acid than in solutions of pure NaCl at the same pH. Citric and picric acids showed a slightly higher corrosion rate, and acetic acid maintained the corrosion rate of pure chloride solutions at the same pH. Organic acids revealed to be weak inhibitors for crevice corrosion. Higher concentration ratios, compared to nitrate ions, were needed to completely inhibit crevice corrosion in chloride solutions. Results are discussed considering acid dissociation constants, buffer capacity and complex formation constants of the different weak acids.

Sosyal Pazarlama Literatür İncelemesi: Prensipler ve Teknikler Kapsamında Ele Alınan Temel Konular (1971-2014)

OpenAIRE

Kitapcı, Olgun; Dörtyol, İbrahim Taylan; Gülmez, Mustafa

2018-01-01

SosyalPazarlama kavramının doğduğu tarihten günümüze yazılmış ve Web of Science (WOS)veri tabanında yayınlanmış makalelerin, Lee ve Kotler’in 2011 yılındayazdıkları kitapta yer alan sınıflandırma çerçevesinde yorumlanmasını amaçlayanbu çalışma sözkonusu alanda zaman içerisinde hakim olan araştırma eğilimlerini ortayakoymaktadır. Bu kapsamda, ilgili veri tabanında yer alan makalelerin tamamıtaranmış ve sınıflandırılmıştır. Makalelerin temel odakları sosyal pazarlamabağlamında ele alınan temel ...

Numerical Tests of the Virtual Human Model Response Under Dynamic Load Conditions Defined in Federal Aviation Regulation Part 23.562 and 25.562 – Preliminary Study

Directory of Open Access Journals (Sweden)

Lindstedt Lukasz

2016-12-01

Full Text Available The main aim of the presented research was to check mechanical response of human body model under loads that can occur during airplane accidents and compare results of analysis with some results of experimental tests described in literature. In simulations, new multi-purpose human body model, the VIRTHUMAN, was used. The whole model, as well as its particular segments, was earlier validated based on experimental data, which proved its accuracy to simulate human body dynamic response under condition typical for car crashes, but it was not validated for loads with predominant vertical component (loads acting along spinal column, typical for airplane crashes. Due to limitation of available experimental data, the authors focused on conducting calculations for the case introduced in 14 CFR: Parts 23.562 and 25.562, paragraph (b(1, knowing as the 60° pitch test. The analysis consists in comparison of compression load measured in lumbar section of spine of the FAA HIII Dummy (experimental model and in the Virthuman (numerical model. The performed analyses show numerical stability of the model and satisfactory agreement between experimental data and simulated Virthuman responses. In that sense, the Virthuman model, although originally developed for automotive analyses, shows also great potential to become valuable tool for applications in aviation crashworthiness and safety analyses, as well.

Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line

Directory of Open Access Journals (Sweden)

Aoranit Somno

2016-01-01

Full Text Available The relationship between P-gp and CD147 in the regulation of MDR in leukemic cells has not been reported. This study aimed to investigate the correlation between CD147 and P-gp in the regulation of drug resistance in the K562/ADR leukemic cell line. The results showed that drug-resistant K562/ADR cells expressed significantly higher P-gp and CD147 levels than drug-free K562/ADR cells. To determine the regulatory effect of CD147 on P-gp expression, anti-CD147 antibody MEM-M6/6 significantly decreased P-gp and CD147 mRNA and protein levels. This is the first report to show that CD147 mediates MDR in leukemia through the regulation of P-gp expression.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on bovine lactoferrin

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to carry out the additional assessment for ‘lactoferrin’ as a food ingredient in the context of Regulation (EC) No 258/97 taking into account the comments and objections...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on bovine lactoferrin

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to carry out the additional assessment of ‘lactoferrin’ as a food ingredient in the context of Regulation (EC) No 258/97 taking into account the comments and objections...

Toprakaltı termiti Heterotermes indicola (Wasmann) (Isoptera: Rhinotermitidae)’ya karşı indoxacarb ve chlorfenapyr’in laboratuvar koşullarında etkisi

OpenAIRE

MISBAH-UL-HAQ, Muhammad; KHAN, Imtiaz Ali; FARID, Abid; ULLAH, Misbah; GOUGE, Dawn H.; BAKER, Paul B.

2016-01-01

Indoxacarb ve chlorfenapyrin’in, Toprakaltı termiti, Heterotermes indicola (Wasmann)’da beslenme engelleyici ve bireyler arasında taşınma etkileri 2013-2014 yıllarında Gıda ve Tarım Nükleer Enstitüsü (NIFA) (Peshawar, Pakistan)’nde laboratuvar testleri ile değerlendirilmiştir. Test edilen kimyasal konsantrasyonları indoxacarb için 1-100 ppm (ağırlık/ağırlık) ve chlorfenapyrin 1 ile 7 ppm (ağırlık/ağırlık) arasında değişmiştir. Gözlemler indoxacarb’ın 50 ppm üzerindeki dozlarda hızlı ölüme seb...

Atık Mermer Tozunun Parke Taşlarında Kullanılması

OpenAIRE

FİLİZ, Mümin; ÖZEL, Cengiz; SOYKAN, Oktar; EKİZ, Yunus

2010-01-01

Bu çalışmada iki farklı çimento tipi (CEM II/B-M (P-LL) 32.5 N ve CEM I 42.5 R) ve iki farklı su/çimento oranında (0.50 ve 0.55) hazırlanmış olan karışımlara mermer tozunun hacim oranınca agrega ile yer değiştirmesinin, mekanik ve fiziksel özelliklere etkisi araştırılmıştır. Elde edilen verilere göre mermer tozu atığının parke taşı imalatında kullanılabilirliği TS 2524 EN 1338 standardına göre değerlendirilmiştir. Elde edilen sonuçlara göre mermer tozu kullanımı ile betonların mekanik ve f...

pH-metric studies on the mixed ligand-chelates of oxovanadium(IV) with 2,2'-bipyridyl and dicarboxylic or hydroxy acids

International Nuclear Information System (INIS)

Jain, A.K.; Kumari, V.; Chaturvedi, G.K.

1978-01-01

The interaction of vanadyl ion with 2,2'-bipyridyl and some dicarboxylic or hydroxy acids (where dicarboxylic acid = oxalic (OX), malonic (MALN), phthalic (PHA), maleic (MAL) acids; hydroxy acids salicylic (SA), 5-sulfosalicylic (5-SSA), mandelic (MAND) and glycollic (HG) acids was studied potentiometrically. pH-titrations of the reaction mixtures containing vanadyl sulphate, 2,2'-bipyridyl and one of the dicarboxylic or hydroxy acids (OX, MALN, PHA, MAL, SA, 5-SSA, MAND and HG acids) in equimolar ratio exhibited the formation of 1:1:1 mixed ligand chelates. The formation constants of the resulting biligand chelates were calculated, at 35 + -1 0 and 45 + -1 0 and also the thermodynamic functions viz. ΔF, ΔH and ΔS (μ=0.1M KNO 3 ) (auth.)

Farklı Bölgelerin Üzümlerinden Üretilen Türk Şaraplarında Resveratrol Düzeyleri

Directory of Open Access Journals (Sweden)

Belkıs Adıgüzel Çaylak

2015-02-01

Full Text Available Resveratrol (3,5,4’ trihidroksistilben, fitoaleksin adı verilen bir polifenol çeşididir. Polifenoller üzümlerin kabuklarında, çekirdeklerinde ve saplarında bulunan maddelerdir. Fenolik bileşikler, özellikle flavanoller şarap alımıyla ilgili bilinen sağlık yararları açısından antioksidan özelliklere sahiptirler. Birçok çalışma, üzümlerde stres koşullarında bir savunma metaboliti olarak sentezlenen resveratrolün şarapta bulunduğunu göstermiştir. Şarap insanlarda kansere, iltihaplanmalara ve koroner kalp hastalıklarına karşı koruyucu özellikleri olduğu bilinen polifenol bileşikleri büyük oranda içermektedir. Hücre ve hayvanlarda yapılan çalışmalar, kırmızı şarabın, lösemi, deri, akciğer ve prostat kanserleri gibi pek çok kanser türünde koruyucu etkisi olduğunu göstermektedir. In-vivo çalışmalardan alınan son bilgiler, resveratrolün kanserin başlangıç, gelişme ve ilerleme safhalarında etkili bir ajan olabileceği hakkında bilgi vermektedir. Farklı ülkelerdeki şarapların içerdiği resveratrol miktarının belirlenmesi ile ilgili birçok araştırma olmasına rağmen, Türkiye’de çok az benzer çalışmalar bulunmaktadır. Bu çalışmada, Türkiye’nin farklı bölgelerinde yetişen üzümlerden üretilen şaraplar önemli şarap üreticilerinden temin edilmiş ve resveratrol miktarları Yüksek Performanslı Sıvı Kromatografisi cihazı kullanılarak belirlenmiştir. Elde edilen sonuçlar resveratrol konsantrasyonunun üzüm çeşidine ve bölgelere göre değişebileceğini göstermiştir.

Apoptosis of leukemia K562 and Molt-4 cells induced by emamectin benzoate involving mitochondrial membrane potential loss and intracellular Ca2+ modulation.

Science.gov (United States)

Yun, Xinming; Rao, Wenbing; Xiao, Ciying; Huang, Qingchun

2017-06-01

Leukemia threatens millions of people's health and lives, and the pesticide-induced leukemia has been increasingly concerned because of the etiologic exposure. In this paper, cytotoxic effect of emamectin benzoate (EMB), an excellent natural-product insecticide, was evaluated through monitoring cell viability, cell apoptosis, mitochondrial membrane potential and intracellular Ca 2+ concentration ([Ca 2+ ] i ) in leukemia K562 and Molt-4 cells. Following the exposure to EMB, cell viability was decreased and positive apoptosis of K562 and Molt-4 cells was increased in a concentration- and time- dependent fashion. In the treatment of 10μM EMB, apoptotic cells accounted for 93.0% to K562 cells and 98.9% to Molt-4 cells based on the control, meanwhile, 63.47% of K562 cells and 81.15% of Molt-4 cells exhibited late apoptotic and necrotic features with damaged cytoplasmic membrane. 48h exposure to 10μM EMB increased significantly the great number of cells with mitochondrial membrane potential (MMP) loss, and the elevation of [Ca 2+ ] i level was peaked and persisted within 70s in K562 cells whilst 50s in Molt-4 cells. Moreover, a stronger cytotoxicity of EMB was further observed than that of imatinib. The results authenticate the efficacious effect of EMB as a potential anti-leukemia agent and an inconsistency with regard to insecticide-induced leukemia. Copyright © 2017 Elsevier B.V. All rights reserved.

Single-molecule Mapping of Long-range Electron Transfer for a Cytochrome b562 Variant

DEFF Research Database (Denmark)

Della Pia, Eduardo Antonio; Chi, Qijin; Jones, D. Dafydd

2011-01-01

Cytochrome b562 was engineered to introduce a cysteine residue at a surface-exposed position to facilitate direct self-assembly on a Au(111) surface. The confined protein exhibited reversible and fast electron exchange with a gold substrate over a distance of 20 Å between the heme redox center an...

pH-metric studies on the mixed ligand-chelates of oxovanadium(IV) with 2,2'-bipyridyl and dicarboxylic or hydroxy acids

Energy Technology Data Exchange (ETDEWEB)

Jain, A K; Kumari, V; Chaturvedi, G K [Agra Coll. (India)

1978-12-01

The interaction of vanadyl ion with 2,2'-bipyridyl and some dicarboxylic or hydroxy acids (where dicarboxylic acid = oxalic (OX), malonic (MALN), phthalic (PHA), maleic (MAL) acids; hydroxy acids salicylic (SA), 5-sulfosalicylic (5-SSA), mandelic (MAND) and glycollic (HG) acids was studied potentiometrically. pH-titrations of the reaction mixtures containing vanadyl sulphate, 2,2'-bipyridyl and one of the dicarboxylic or hydroxy acids (OX, MALN, PHA, MAL, SA, 5-SSA, MAND and HG acids) in equimolar ratio exhibited the formation of 1:1:1 mixed ligand chelates. The formation constants of the resulting biligand chelates were calculated, at 35/sup +/-1/sup 0/ and 45/sup +/-1/sup 0/ and also the thermodynamic functions viz. ..delta..F, ..delta..H and ..delta..S (..mu..=0.1M KNO/sub 3/) (auth.).

Interactive CD based training on NDA instruments for facility operators and international inspectors

International Nuclear Information System (INIS)

Horley, E.C.; Smith, H.A.

1996-01-01

Interactive multimedia training is rapidly becoming a popular and highly effective medium for learning. An interactive CD based training module on the Active Well Coincidence counter is being developed for on-site training at nuclear facility, including foreign facilities. The training module incorporates interactive text, graphics and video that demonstrate the operating principles, and the use and set-up of the instrument. The user is in control of the pace of learning and of the directions taken to acquire information based on personal need. By being in control, the user stays highly motivated. A mix of visuals (text and graphics), audio clips (in different languages), and video (with audio) clips also keeps the interest level high. Skill reviews and evaluations can be incorporated into the training to provide feedback to the student. In addition, general background information is provided on gamma and neutron based MC and A measurements. This material serves as a condensed MC and A encyclopedia. By supplying an interactive CD with an NDA instrument, nuclear facilities will have greater assurance operators are properly trained in the set-up and operation of the NDA-equipment

Redox tuning of cytochrome b₅₆₂ through facile metal porphyrin substitution

DEFF Research Database (Denmark)

Della Pia, Eduardo Antonio; Chi, Qijin; Elliott, Martin

2012-01-01

The biologically and nanotechnologically important heme protein cytochrome b562 was reconstructed with zinc and copper porphyrins, leading to significant changes in the spectral, redox and electron transfer properties. The Cu form shifts the redox potential by +300 mV and exhibits high electron t...

Bcr-Abl-independent mechanism of resistance to imatinib in K562 cells: Induction of cyclooxygenase-2 (COX-2) by histone deacetylases (HDACs).

Science.gov (United States)

Kalle, Arunasree M; Sachchidanand, Sachchidanand; Pallu, Reddanna

2010-09-01

Our previous studies have shown that overexpression of MDR1 and cyclooygenase-2 (COX-2) resulted in resistance development to imatinib in chronic myelogenous leukemia (CML) K562 (IR-K562) cells. In the present study, the regulatory mechanism of MDR1 induction by COX-2 was investigated. A gradual overexpression of MDR1 and COX-2 during the process of development was observed. Furthermore, down regulation of MDR1 upon COX-2 knockdown by siRNA showed a decrease in the PKC levels and activation of PKC by addition of PGE(2) to K562 cells, suggesting a role for PKC in the COX-2 mediated induction of MDR1. The present study demonstrates COX-2 induction by HDACs and MDR1 induction by COX-2 via PGE(2)-cAMP-PKC-mediated pathway. Copyright 2010 Elsevier Ltd. All rights reserved.

Meyve Konservelerinde ve Sularında Bozulmalara Neden Olan Küf Mantarları

Directory of Open Access Journals (Sweden)

Jale Acar

2015-02-01

Full Text Available Bakteriler, meyve suları ve konservelerinin bozulmalarında pek önemli rol oynamadıkları halde mayalar ve özellikle küf mantarları bu bozulmalarda önemli bir yer alırlar. Çeşitli mikroorganizmaların gelişme isteklerinin farklı olması buna neden olmaktadır. Küf mantarlarının büyük bir kısmı ancak aerob koşullar altında gelişebilirler. Bu gelişme en fazla yüzeydedir. Konserve kutularının hermetikli olan kapatılması ve tepe boşluğunda çok az oksijen bulunması yüzeyde küf mantarlarının gelişmesini önlemekle beraber Byssochlamys cinsinden küf mantarları anaerob koşullar altında fazla olmasa bile gelişebilmektedirler. Küf mantarları bakterilere oranla az miktarda suya gereksinim duyarlar. Aspergillus glaucus ve birçok Penicillium türleri kserofil olup aktif suyun çok düşük olduğu 0.70-0.75 gelişebilmelerine karşın bu değer bakterilerde 0.90-0.98 arasındadır. Çok az miktardaki su örneğin, yüzeydeki kondense su pastörizasyonda öldürülemeyen veya sonradan herhangi bir şekilde reçel kabına giren küflerin gelişmesine yardımcı olabilir. Sonradan bulaşma özellikle evlerde yapılan ve soğuduktan sonra kapağı kapatılan, özellikle reçel kabı selofan kağıdı ile kapatılıyorsa, sık sık görülmektedir. Diğer taraftan kaplar reçel soğutulmadan kapatılacak olursa suyun kondense olma miktarı artmaktadır.

Antiproliferative Activity of β-Hydroxy-β-Arylalkanoic Acids

Directory of Open Access Journals (Sweden)

Ivan O. Juranić

2007-03-01

Full Text Available Article describes the synthesis of fifteen β-hydroxy-β-arylalkanoic acids byReformatsky reaction using the 1-ethoxyethyl-2-bromoalkanoates, aromatic or cycloalkylketones or aromatic aldehydes. The short survey of previously reported synthetic proceduresfor title compounds, is given. The majority of obtained compounds exert antiproliferativeactivity in vitro toward human: HeLa, Fem-X cells, K562, and LS174 cells, having IC50values from 62.20 to 205 μM. The most active compound is 3-OH-2,2-di-Me-3-(4-biphenylyl-butanoic acid, having the IC50 value 62.20 μM toward HeLa cells. Sevenexamined compounds did not affect proliferation of healthy human blood peripheralmononuclear cells (PBMC and PBMC PHA, IC50 > 300 μM. The preliminary QSARresults show that estimated lipophilicity of compounds influences their antiproliferativeactivity in the first place. The ability of dehydration, and the spatial arrangement ofhydrophobic portion, HBD and HBA in molecules are has almost equal importance aslipophilicity.

Probing the location of displayed cytochrome b562 on amyloid by scanning tunnelling microscopy

International Nuclear Information System (INIS)

Forman, C J; Barker, P D; Wang, N; Durkan, C; Yang, Z Y; Mowat, C G; Jarvis, S

2013-01-01

Amyloid fibres displaying cytochrome b 562 were probed using scanning tunnelling microscopy (STM) in vacuo. The cytochromes are electron transfer proteins containing a haem cofactor and could, in principle, mediate electron transfer between the tip and the gold substrate. If the core fibres were insulating and electron transfer within the 3D haem network was detected, then the electron transport properties of the fibre could be controlled by genetic engineering. Three kinds of STM images were obtained. At a low bias ( 562 was not detected by STM, which was attributed to low adhesion, whereas a monomeric multi-haem protein, GSU1996, was readily imaged. We conclude that the fibre superstructure may be intermittently conducting, that the cytochromes have been seen within the fibres and that they are too far apart for detectable current flow between sites to occur. We predict that GSU1996, being 10 nm long, is more likely to mediate successful electron transfer along the fibre as well as being more readily detectable when displayed from amyloid. (paper)

Ruminations On NDA Measurement Uncertainty Compared TO DA Uncertainty

International Nuclear Information System (INIS)

Salaymeh, S.; Ashley, W.; Jeffcoat, R.

2010-01-01

It is difficult to overestimate the importance that physical measurements performed with nondestructive assay instruments play throughout the nuclear fuel cycle. They underpin decision making in many areas and support: criticality safety, radiation protection, process control, safeguards, facility compliance, and waste measurements. No physical measurement is complete or indeed meaningful, without a defensible and appropriate accompanying statement of uncertainties and how they combine to define the confidence in the results. The uncertainty budget should also be broken down in sufficient detail suitable for subsequent uses to which the nondestructive assay (NDA) results will be applied. Creating an uncertainty budget and estimating the total measurement uncertainty can often be an involved process, especially for non routine situations. This is because data interpretation often involves complex algorithms and logic combined in a highly intertwined way. The methods often call on a multitude of input data subject to human oversight. These characteristics can be confusing and pose a barrier to developing and understanding between experts and data consumers. ASTM subcommittee C26-10 recognized this problem in the context of how to summarize and express precision and bias performance across the range of standards and guides it maintains. In order to create a unified approach consistent with modern practice and embracing the continuous improvement philosophy a consensus arose to prepare a procedure covering the estimation and reporting of uncertainties in non destructive assay of nuclear materials. This paper outlines the needs analysis, objectives and on-going development efforts. In addition to emphasizing some of the unique challenges and opportunities facing the NDA community we hope this article will encourage dialog and sharing of best practice and furthermore motivate developers to revisit the treatment of measurement uncertainty.

RUMINATIONS ON NDA MEASUREMENT UNCERTAINTY COMPARED TO DA UNCERTAINTY

Energy Technology Data Exchange (ETDEWEB)

Salaymeh, S.; Ashley, W.; Jeffcoat, R.

2010-06-17

It is difficult to overestimate the importance that physical measurements performed with nondestructive assay instruments play throughout the nuclear fuel cycle. They underpin decision making in many areas and support: criticality safety, radiation protection, process control, safeguards, facility compliance, and waste measurements. No physical measurement is complete or indeed meaningful, without a defensible and appropriate accompanying statement of uncertainties and how they combine to define the confidence in the results. The uncertainty budget should also be broken down in sufficient detail suitable for subsequent uses to which the nondestructive assay (NDA) results will be applied. Creating an uncertainty budget and estimating the total measurement uncertainty can often be an involved process, especially for non routine situations. This is because data interpretation often involves complex algorithms and logic combined in a highly intertwined way. The methods often call on a multitude of input data subject to human oversight. These characteristics can be confusing and pose a barrier to developing and understanding between experts and data consumers. ASTM subcommittee C26-10 recognized this problem in the context of how to summarize and express precision and bias performance across the range of standards and guides it maintains. In order to create a unified approach consistent with modern practice and embracing the continuous improvement philosophy a consensus arose to prepare a procedure covering the estimation and reporting of uncertainties in non destructive assay of nuclear materials. This paper outlines the needs analysis, objectives and on-going development efforts. In addition to emphasizing some of the unique challenges and opportunities facing the NDA community we hope this article will encourage dialog and sharing of best practice and furthermore motivate developers to revisit the treatment of measurement uncertainty.

Tarihsel süreç içinde peyzaj tasarım ve uygulama çalışmalarında yapı malzemelerinin kullanımının irdelenmesi

OpenAIRE

Shakouri, Nasim

2010-01-01

Peyzaj, sayısız materyallerden oluşan görsel bir bütünlük olarak tanımlanmaktadır. Bu materyaller zaman içindeki değişimlerle, peyzaj tipi ve karakterini belirlemektedir. Peyzaj mimarlığında kullanılan materyaller, canlı ve cansız materyaller olarak ayrıca tanımlanmaktadır. Cansız materyaller kavramı içinde, peyzaj mimarlığında kullanılan yapısal öğelerin oluşturmasında önemli rol oynayan yapı malzemeleri de yer almaktadır. Peyzaj mimarlığında kullanılan yapısal malzemelerin özellikl...

Lactococcus lactis Subsp. Lactis Suşlarında Yüksek Sıklıkta Konjugal Transfer Sistemlerinin Analizi

Directory of Open Access Journals (Sweden)

Çağla Tükel

2015-02-01

Full Text Available Bu çalışmada L. lactis subsp. lactis suşlarında laktoz fermentasyonu özelliğini kodlayan altı farklı plazmidin yüksek sıklıkta konjugal aktarım yeteneği araştırıldı. Bu plazmidlerin konjugal transfer sıklıkları; iki seks faktörünün interaksiyonuna bağlı olarak (Clu ve Agg, Clu-/Agg-, Agg+ x Clu-/Agg+, Agg- ya da Clu+/Agg- x Clu-/Agg- konjugasyon eşleri için 1.5x10-5–1.0x10-7 ve Clu+/Agg- x Clu-/Agg+ konjugasyon eşleri için 7.1x10-2-2.7x10-3 oranlarında değişim gösterdi. Laktoz plazmidlerinin stabiliteleri ise; doğal suşlarda %82-96, MG1390 alıcı suşu için tanımlanan konjugantlarda %77-98 ve MCL8060 alıcı suşu için tanımlanan konjugantlarda ise %44-67 arasında saptandı.

Domates Pulpu ve Salçasında Viskozite (Konsistens ve Renk Üzerine Proses Koşullarının Etkisi

Directory of Open Access Journals (Sweden)

Aziz Ekşi

2015-02-01

Full Text Available Kıvam ve renk, domates pulpu ve salçada kaliteyi belirleyen ve ticarette üzerinde en çok durulan iki önemli etkendir. Domates salçasında renk ve kıvam ile hammaddenin durumu arasında yakın bir ilişki bulunduğu bilinmektedir. Ancak her iki kalite öğesini ve özellikle kıvamı, hammadde olduğu kadar, proses koşulları da etkilemektedir.

İçme ve sulama sularında bor kirliliği ve bor giderme yöntemleri

Directory of Open Access Journals (Sweden)

Meltem Bilici Başkan

2014-03-01

Full Text Available Periyodik cetvelin 3A grubunda yer alan bor yüksek iyonlaşma potansiyeline sahip olması nedeniyle yarı metal olarak kabul edilir. Yerkabuğundaki ortalama bor konsantrasyonu 10 mg/kg'dır. Bor doğada Ca, Na ve Mg'un tuzları şeklinde bulunur. Yüksek konsantrasyonlarda ve ekonomik boyutlardaki bor yatakları; borun oksijen ile bağlanmış bileşikleri şeklinde daha çok Türkiye ve ABD'nin kurak, volkanik ve hidrotermal aktivitesi olan bölgelerinde bulunmaktadır. Bor, bitkiler için gerekli bir mikro besin elementi olmanın yanı sıra, gerekli tüm besin elementleri içerisinde, eksiklik belirtilerine neden olan miktarı ile toksik etki yapan miktarı, birbirine çok yakın olan tek elementtir. Bor su ortamında genellikle borik asit ve kısmen de borat tuzları formlarında bulunmaktadır. Pek çok endüstride kullanım alanı bulan bor bileşiklerinin endüstriyel gelişmelere bağlı olarak yüzeysel sularda bulunan konsantrasyonu artış göstermektedir. Yüzeysel sularda bulunan bor aynı zamanda evsel atıksulardan da kaynaklanmaktadır. Türkiye'de içme ve sulama sularını en çok kirleten toksik elementlerin başında bor gelmektedir. Bu nedenle bor giderimi hem insan sağlığı açısından hem de sağlıklı tarım ürünü yetiştirilebilmesi açısından oldukça önemlidir. Sulardan bor giderimi için uygulanan temel yöntemler arasında iyon değişimi, ultrafiltrasyon, ters osmoz ve adsorbsiyon gelmektedir.

Fast critical assembly safeguards: NDA methods for highly enriched uranium. Summary report, October 1978-September 1979

International Nuclear Information System (INIS)

Bellinger, F.O.; Winslow, G.H.

1980-12-01

Nondestructive assay (NDA) methods, principally passive gamma measurements and active neutron interrogation, have been studied for their safeguards effectiveness and programmatic impact as tools for making inventories of highly enriched uranium fast critical assembly fuel plates. It was concluded that no NDA method is the sole answer to the safeguards problem, that each of those emphasized here has its place in an integrated safeguards system, and that each has minimum facility impact. It was found that the 185-keV area, as determined with a NaI detector, was independent of highly-enriched uranium (HEU) plate irradiation history, though the random neutron driver methods used here did not permit accurate assay of irradiated plates. Containment procedures most effective for accurate assaying were considered, and a particular geometry is recommended for active interrogation by a random driver. A model, pertinent to that geometry, which relates the effects of multiplication and self-absorption, is described. Probabilities of failing to detect that plates are missing are examined

Co-ordinate expression of activin A and its type I receptor mRNAs during phorbol ester-induced differentiation of human K562 erythroleukemia cells.

Science.gov (United States)

Hildén, K; Tuuri, T; Erämaa, M; Ritvos, O

1999-07-20

Activins were originally isolated based on their ability to stimulate follicle-stimulating hormone secretion but later they have been shown to regulate a number of different cellular functions such as nerve cell survival, mesoderm induction during early embryogenesis as well as hematopoiesis. We studied the regulation of activin A, a homodimer of betaA-subunits, mRNA and protein in K562 erythroleukemia cells, which are known to be induced toward the erythroid lineage in response to activin or TGF-beta or toward the megakaryocytic lineage by the phorbol ester protein kinase C activator 12-O-tetradecanoylphorbol-13-acetate (TPA). Here we show by Northern blot analysis as well as by Western and ligand blotting that TPA strongly promotes activin betaA-subunit mRNA and activin A protein expression in K562 cells in time- and concentration dependent manner. In contrast, neither activin A nor TGF-beta induced betaA-subunit mRNA expression during erythroid differentiation in K562 cells. Interestingly, whereas activin type II receptors are not regulated during K562 cell differentiation (Hilden et al. (1994) Blood 83, 2163-2170), we now show that the activin type I and IB receptor mRNAs are clearly induced by TPA but not by activin or TGF-beta. We also show that the inducing effect of TPA on expression of activin betaA-subunit mRNA is potentiated by the protein kinase A activator 8-bromo-cAMP. We conclude that activin A and its type I receptors appear to be co-ordinately up-regulated during megakaryocytic differentiation of K562 cells.

Derin suda dairesel silindir arkasında meydana gelen akış yapısının örgü teller ile kontrol edilmesi

Directory of Open Access Journals (Sweden)

Burcu Oğuz

2016-08-01

Full Text Available Bu çalışmada amaç derin su içerisine yerleştirilen silindire (bacalar, yüksek binalar vb. ve arkasındaki bir cisme veya cisimlere girdap kopması sonucu etki eden daimi olmayan yüklerin etkisinin ve şiddetinin azaltılması ve optimum değerlerin (tel kalınlığı ve geçirgenlik oranı, β belirlenmesidir. Bu amaçla çapı D=50 mm olan dairesel silindir etrafına sarılan farklı kalınlık ve geçirgenlik oranına sahip örgü tellerin etkisi Re_D=5000 değerinde araştırılmıştır. Geçirgenlik oranları β=0.5-0.8 aralığında 0.1 artımlarla dört farklı değerdedir. Silindir üzerine sarılmış tellerin kalınlıkları b=1 mm, 2 mm, 3mm ve 4mm’dir. Silindir arkasında meydana gelen akış yapısı, silindir etrafına sarılan örgü tellerle kontrol edilmeye çalışılmıştır. Deneyler PIV (parçacık görüntülemeli hız ölçme tekniği kullanılarak yürütülmüştür. Tüm geçirgenlik oranlarında, Türbülans Kinetik Enerji (TKE ve Reynolds kayma gerilmesi değerleri b=1 mm ve 2 mm tel kalınlıklarında, örgüsüz silindirin değeri ile kıyaslandığında artmakta fakat b=3 mm ve 4 mm kalınlıklarında azalmaktadır. Geçirgenlik oranı, β=0.6 değerinde ve tel kalınlığı b=4 mm durumunda TKE ve Reynolds kayma gerilmesi değerleri, örgü tellerinin silindir arkasında meydana gelen akışı kontrol ettiğini göstermektedir. Frekans değerlerinin sonuçları da örgü tellerin, silindir arkasındaki akışı, geçirgenlik oranı β=0.6 ve b=4 mm tel kalınlığında en iyi kontrolü sağladığını net bir şekilde açıklamaktadır

İstanbul’da 1999 Yılında Sonuçlanmış Boşanma Davalarında Boşanma Nedenlerinin Değerlendirilmesi

Directory of Open Access Journals (Sweden)

Halim Dişsever

2000-12-01

Full Text Available Bu çalışmada İstanbul'da 1999 yılında sonuçlanmış boşanma davalarından rastlantısal örnekleme yöntemiyle seçilen 3060 boşanma olgusu incelenerek, boşanma nedenleri ve diğer değişkenler arasındaki ilişkilerin değerlendirilmesi amaçlanmıştır İncelenen ve davası sonuçlanmış toplam 3060 boşanma davasının %67.1’si kailin (2053, %32.9'u (1007 erkek taralından açılmıştır, boşanma nedenlerine göre dağılım incelendiğinde ilk sırayı şiddetli geçimsizlik, ikinci sırayı alkol kötüye kullanımı, üçüncü sırayı cana kast ve pek fena muamele. i sırayı ise terk boşanma nedeninin aldığı görülmektedir. Boşanan çiftlerin %41.6 sı (1272 çift çocuksuz olup. %40.4 ü (12.36 çift evliliklerinin ilk 5 yılı içinde boşanmışlardır. Çocukların çoğunun yaşları küçük olduğundan velayetlerin annelere verilme oranı daha yüksektir. Olguların %7.6’smıla (233 çift eşlerden birinin, %28.2’ sinde (864 çift her ikisinin İstanbul nüfusuna kayıtlı olduğu, %64.2’sinde (196.3 çift her ikisinin nüfus kaydının İstanbul dışında olduğu saptanmıştır. Boşanma sonrası eşler ve çocukları ruhsal ve ekonomik sorunlarla karşı karşıya kalmaktadır. Sosyal güvenceleri, eşlerin nafaka durumları, işsizlik ve çocukların eğitini durumu göz önüne alındığında sosyal destek mekanizmalarının daha ila güçlendirilmesi toplum ruh sağlığı açısından faydalı olacaktır. Anahtar Kelimeler: Boşanma, boşanma nedenleri.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on Dietary Reference Values for energy

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived dietary reference values for energy, which are provided as average requirements (ARs) of specified age and sex groups. For children and adults, total energy expenditure (TEE...

Nephrotoxicity of mercapturic acids of three structurally related 2,2-difluoroethylenes in the rat. Indications for different bioactivation mechanisms.

NARCIS (Netherlands)

Commandeur, J.N.M.; Brakenhoff, J.P.G.; de Kanter, F.J.J.; Vermeulen, N.P.E.

1988-01-01

The biotransformation and the hepato- and nephrotoxicity of the mercapturic acids (N-acetyl-1-cysteine S-conjugates) of three structurally related 2,2-difluoroethylenes were investigated in vivo in the rat. All mercapturic acids appeared to cause nephrotoxicity, without any measureable effect on the

Nasturi Toplumunda Hayvan Yetiştiriciliği ve Ticareti (XIX. Yüzyılın İkinci Yarısında)

OpenAIRE

DALYAN, Murat Gökhan

2010-01-01

XIX. Yüzyılda Hakkâri ve Urmiye bölgesinde yaşayan Nasturiler arasında ekonomik olarak hayvancılık mühim bir yer işgal etmiştir. Beslenen hayvanların tür olarak dağılışında genel olarak coğrafi ve tarım alanlarının yapısı etkili olmuştur. Bu bağlamda tarım alanlarının fazla olduğu Urmiye’de büyükbaş hayvancılık gerçekleştirilirken; Hakkâri dağlarında ise daha çok küçükbaş hayvancılık yapılmıştır. Yük hayvanı olarak da özelikle engebeli arazi yapısından dolayı katır beslenmiştir. Nasturiler ar...

Küresel Ekonomik Kriz Türkiye İşgücü Piyasasında Cinsiyet Eşitsizliğini Derinleştirdi mi?

OpenAIRE

ÇETİNKAYA, Emel; YILDIRIMALP, Sinem

2013-01-01

İşgücü piyasalarında cinsiyet eşitsizliği, tüm dünyada olduğu gibi Türkiye işgücü piyasasında da önemli bir sorun olarak karşımıza çıkmaktadır. Zaman zaman yaşanan krizler işgücü piyasalarını ve özellikle kadın işgücünü olumsuz yönde etkilemektedir. 2007 yılında ABD’de başlayan küresel ekonomik kriz tüm ülke ekonomileri gibi Türkiye ekonomisini ve işgücü piyasalarını da 2008 yılının özellikle ikinci yarısından itibaren ciddi bir şekilde etkilemeye başlamıştır. Bu çalışma, küresel ekonomik kri...

Synergistic effect of hydrogen peroxide on polyploidization during the megakaryocytic differentiation of K562 leukemia cells by PMA.

Science.gov (United States)

Ojima, Yoshihiro; Duncan, Mark Thompson; Nurhayati, Retno Wahyu; Taya, Masahito; Miller, William Martin

2013-08-15

The human myelogenous cell line, K562 has been extensively used as a model for the study of megakaryocytic (MK) differentiation, which could be achieved by exposure to phorbol 12-myristate 13-acetate (PMA). In this study, real-time PCR analysis revealed that the expression of catalase (cat) was significantly repressed during MK differentiation of K562 cells induced by PMA. In addition, PMA increased the intracellular reactive oxygen species (ROS) concentration, suggesting that ROS was a key factor for PMA-induced differentiation. PMA-differentiated K562 cells were exposed to hydrogen peroxide (H2O2) to clarify the function of ROS during MK differentiation. Interestingly, the percentage of high-ploidy (DNA content >4N) cells with H2O2 was 34.8±2.3% at day 9, and was 70% larger than that without H2O2 (21.5±0.8%). Further, H2O2 addition during the first 3 days of PMA-induced MK differentiation had the greatest effect on polyploidization. In an effort to elucidate the mechanisms of enhanced polyploidization by H2O2, the BrdU assay clearly indicated that H2O2 suppressed the division of 4N cells into 2N cells, followed by the increased polyploidization of K562 cells. These findings suggest that the enhancement in polyploidization mediated by H2O2 is due to synergistic inhibition of cytokinesis with PMA. Although H2O2 did not increase ploidy during the MK differentiation of primary cells, we clearly observed that cat expression was repressed in both immature and mature primary MK cells, and that treatment with the antioxidant N-acetylcysteine effectively blocked and/or delayed the polyploidization of immature MK cells. Together, these findings suggest that MK cells are more sensitive to ROS levels during earlier stages of maturation. Copyright © 2013 Elsevier Inc. All rights reserved.

Yoğurt Yapımında Yararlanılan L. bulgaricus ve S. thermophilus’un Proteolitik Aktivitelerinin Belirlenmesi

Directory of Open Access Journals (Sweden)

Sevda Kılıç

2015-02-01

Full Text Available Yoğurt bakterilerinin süte aşılandıktan sonra enzimatik etkinlikleri sonucu proteinleri parçalamaları olayına proteoliz adı verilir. Bu olay sırasında ortamda gerekli gelişme faktörleri meydana geldiğinden S. thermophilus’un gelişmesi ve çoğalması hızlandırılmış olur. Simbiyotik faaliyet gösteren bu bakteriler için gerekli ve yararlı olan proteoliz olayının yoğurt yapımında ancak belirli bir düzeyde meydana gelmesi istenir. ASPERGER, tirosin aminoasiti ile aroma oluşumu arasında bir ilişkinin olduğunu, tirosin içeriğinin 0.05-0.1 mg/ml olması durumunda yoğurtların istenen aromaya sahip olabileceğini bildirmiştir. 0.125 mg/ml tirosin içeren yoğurt örneklerinde ise hafif acı tat oluştuğu bildirilmiştir. Bundan hareketle yoğurt bakterileri olan L. bulgaricus ile S. thermophilus’ a ait suşların proteolitik aktiviteleri HULL yöntemine göre tirosin ekivalantı üzerinden belirlenmeye çalışılmıştır. Araştırmada genellikle yüksek asitlik oluşturabilen L. bulgaricus suşlarının daha fazla proteolitik aktivite gösterdikleri saptanmıştır. Ancak bütün bakteri suşları için bu durumun geçerli olmadığı da belirlenmiştir. 20 L. bulgaricus suşuna ait değerlerin 0.105-0.215 mg tirosin/ml sınırları arasında değiştiği gözlenmiştir. S. thermophilus’un denemeye alınan 20 suşu için saptanan değerler de 0.080-0.070 mg tirosin/ml arasında belirlenmiş olup değerlerin L. bulgaricus’ a ait değerlerden çok düşük olduğu anlaşılmıştır.

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on Dietary reference values for water

DEFF Research Database (Denmark)

Tetens, Inge

This Opinion of the EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA) deals with the setting of dietary reference values for water for specific age groups. Adequate Intakes (AI) have been defined derived from a combination of observed intakes in population groups with desirable...

Bıldırcın ve Ördek Yumurtalarında Maya-Küf ve Total Bakteri Açısından İncelenmesi

Directory of Open Access Journals (Sweden)

Işın Var

2015-02-01

Full Text Available Bıldırcın yumurtası, besin değeri yönünden tavuk yumurtasından oransal olarak daha zengin kabul edilmektedir. Daha çok salata, pasta, mayonez vb. yapımında yaygın olarak kullanılan ördek yumurtası (Pekin ördeği tavuk yumurtasına göre daha büyük olup, ağırlıkları 70-90 g arasında değişmektedir. Bıldırcın yumurtası son yıllarda özellikle küçük çocuk ve yaşlı beslenmesinde önem kazanmıştır. Bunun yanısıra bazı hastalıkların tedavisi amacıyla da halk arasında oldukça yaygın kullanılmaktadır. Özellikle çiğ olarak tüketilen bıldırcın yumurtaları zaman zaman içerdikleri patojen mikroorganizmalarla tehlikeli olabilmektedirler. Bu çalışmada bıldırcın ve ördek yumurtalarında maya-küf ve total bakteri aranmıştır. Bıldırcın ve ördek yumurtalarının özellikle kabuğunda hem total bakteri hem de maya-küf bulunmuştur. Bıldırcın yumurtasının akı ve sarısında ise zaman zaman kontaminasyonla karşılaşılmıştır.

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on establishing Food-Based Dietary Guidelines

DEFF Research Database (Denmark)

Tetens, Inge

This Opinion of the EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA) provides guidance on the translation of nutrient based dietary advice into guidance, intended for the European population as a whole, on the contribution of different foods or food groups to an overall diet...

Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells

OpenAIRE

Takuya Yamane; Tatsuji Sakamoto; Takenori Nakagaki; Yoshihisa Nakano

2018-01-01

The Japanese fermented beverage, homemade kefir, contains six lactic acid bacteria: Lactococcus. lactis subsp. Lactis, Lactococcus. lactis subsp. Cremoris, Lactococcus. Lactis subsp. Lactis biovar diacetylactis, Lactobacillus plantarum, Leuconostoc meseuteroides subsp. Cremoris and Lactobacillus casei. In this study, we found that a mixture of the six lactic acid bacteria from kefir increased the cytotoxicity of human natural killer KHYG-1 cells to human chronic myelogenous leukemia K562 cell...

Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro.

Science.gov (United States)

Brisdelli, Fabrizia; Perilli, Mariagrazia; Sellitri, Doriana; Bellio, Pierangelo; Bozzi, Argante; Amicosante, Gianfranco; Nicoletti, Marcello; Piovano, Marisa; Celenza, Giuseppe

2016-08-01

The aim of this study was to investigate the effect of protolichesterinic acid, a lichen secondary metabolite, on anti-proliferative activity of doxorubicin in three human cancer cell lines, HeLa, SH-SY5Y and K562 cells. The data obtained from MTT assays, performed on cells treated with protolichesterinic acid and doxorubicin alone and in combination, were analysed by the median-effect method as proposed by Chou and Talalay and the Bliss independence model. Apoptosis rate was evaluated by fluorescence microscopy, caspase-3, 8 and 9 activities were detected by spectrofluorimetric analysis and protein expression of Bim, Bid, Bax and Mcl-2 was analysed by Western blotting. The interaction of protolichesterinic acid with thioesterase domain of human fatty acid synthase (hFAS) was investigated by a molecular docking study. The in vitro activity of doxorubicin against HeLa cancer cell line, but not against SH-SY5Y and K562 cells, was synergically increased by protolichesterinic acid. The increased cytotoxicity caused by protolichesterinic acid in HeLa cells was due to a pro-apoptotic effect and was associated to caspase-3, 8 and 9 activation. The simultaneous treatment for 24h with protolichesterinic acid plus doxorubicin caused an increase of Bim protein expression and the appearance of cleaved form of Bid protein. The molecular modelling analysis showed that protolichesterinic acid seemed to behave as a competitive inhibitor of hFAS. These results suggest that protolichesterinic acid could be envisaged as an useful tool against certain types of tumor cells in combination with anticancer drugs. Copyright © 2016 Elsevier Inc. All rights reserved.

Spectrographic study of neodymium complexing with hydroxyethylidenediphosphonic acid in acid media

International Nuclear Information System (INIS)

Afonin, E.G.; Pechurova, N.I.; Martynenko, L.I.

1987-01-01

High resolution spectrography is used to study neodymium (3) complexing with hydroxyethylidenediphosphonic acid (H 4 X) in aqueous solution with hydrogen ion concentration being 0.35-4.5 g-ion/l. It is shown that complex of NdH 2 X + composition characterized by λ=4290 A absorption band in electron spectrum in transition range 4 I 9/2 → 2 P 1/2 is formed in a system. The lgK stab =5.62 ± 0.07 value is calculated. It is supposed that high stability of a complex is conditioned by participation of ligand nondissociated alcohol group in coordination

Quinacrine induces apoptosis in human leukemia K562 cells via p38 MAPK-elicited BCL2 down-regulation and suppression of ERK/c-Jun-mediated BCL2L1 expression

International Nuclear Information System (INIS)

Changchien, Jung-Jung; Chen, Ying-Jung; Huang, Chia-Hui; Cheng, Tian-Lu; Lin, Shinne-Ren; Chang, Long-Sen

2015-01-01

Although previous studies have revealed the anti-cancer activity of quinacrine, its effect on leukemia is not clearly resolved. We sought to explore the cytotoxic effect and mechanism of quinacrine action in human leukemia K562 cells. Quinacrine induced K562 cell apoptosis accompanied with ROS generation, mitochondrial depolarization, and down-regulation of BCL2L1 and BCL2. Upon exposure to quinacrine, ROS-mediated p38 MAPK activation and ERK inactivation were observed in K562 cells. Quinacrine-induced cell death and mitochondrial depolarization were suppressed by the p38MAPK inhibitor SB202190 and constitutively active MEK1 over-expression. Activation of p38 MAPK was shown to promote BCL2 degradation. Further, ERK inactivation suppressed c-Jun-mediated transcriptional expression of BCL2L1. Over-expression of BCL2L1 and BCL2 attenuated quinacrine-evoked mitochondrial depolarization and rescued the viability of quinacrine-treated cells. Taken together, our data indicate that quinacrine-induced K562 cell apoptosis is mediated through mitochondrial alterations triggered by p38 MAPK-mediated BCL2 down-regulation and suppression of ERK/c-Jun-mediated BCL2L1 expression. - Highlights: • Quinacrine induces K562 cell apoptosis via down-regulation of BCL2 and BCL2L1. • Quinacrine induces p38 MAPK activation and ERK inactivation in K562 cells. • Quinacrine elicits p38 MAPK-mediated BCL2 down-regulation. • Quinacrine suppresses ERK/c-Jun-mediated BCL2L1 expression

Roma Hukuku’nda Gemi, Han ve Ahır İşletenlerin Receptum Sorumluluğu

Directory of Open Access Journals (Sweden)

Mehmet YEŞİLLER

2013-04-01

Full Text Available Çalışmamızda Roma Hukuku'nda gemi, han ve ahır işleten kimselerin sorumluluklarına ilişkin düzenlemeler ele alınmıştır. Roma?da özellikle gemi, han ve ahır işleten kimselerin yanlarında çalıştırdıkları kişilerin güvenilir olmamasından dolayı, taraflar arasındaki istisna sözleşmesinden kaynaklanan custodia sorumluluğuna ek olarak "actio de damno aut furto adversus nautas, caupones, stabularios ve receptum, nautae, cauponis, stabularii" sorumluluklarının düzenlendiği kaynaklardan anlaşılmaktadır. Praetor Edictum'larıyla sağlanan ve haksız fiil benzerlerine dayanan söz konusu bu uygulamaların, Roma'da istisna sözleşmesinin koruyamadığı alana ilişkin olarak geniş uygulama imkanı buldukları görülmektedir.

Detection of pyrrolizidine alkaloids using flow analysis with both acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence

International Nuclear Information System (INIS)

Gorman, Bree A.; Barnett, Neil W.; Bos, Richard

2005-01-01

For the first time, analytically useful chemiluminescence was elicited from the reactions of the pyrrolizidine alkaloids. Heliotrine, retronecine, supinine, monocrotaline and echinatine N-oxide yielded chemiluminescence upon reaction with tris(2,2'-bipyridyl)ruthenium(II) whilst lasiocarpine, its N-oxide and supinine elicited light upon reaction with acidic potassium permanganate. Detection limits for heliotrine were 1.25 x 10 -7 M and 9 x 10 -9 M for tris(2,2'-bipyridyl)ruthenium(III) perchlorate with flow injection analysis (FIA) and the silica-immobilised reagent (4-[4-(dichloromethylsilanyl)-butyl]-4'-methyl-2,2'-bipyridine)bis (2,2'-bipyridyl)ruthenium(II) with sequential injection analysis (SIA), respectively. Lasiocarpine was detectable at 1.4 x 10 -7 M using acidic potassium permanganate with FIA. Additionally, the silica-immobilised reagent was optimised with respect to the oxidant (ammonium ceric nitrate) concentration and the aspiration times which afforded a detection limit for codeine of 5 x 10 -10 M using SIA

Sakarya Özel Eğitim Kurumunda Eğitim Gören Çocukların Beslenme Tarzında Ebeveynlerin Bilgi ve Tutumlarının İncelenmesi

Directory of Open Access Journals (Sweden)

Süleyman KALELİ

2017-06-01

Full Text Available Beslenme büyüme ve gelişimin sağlanması, sağlıklı bir yaşam ve sağlıklı bir gelecek için çocukluk evresinde çok önemlidir. Otizm ve down sendromu bir takım benzerlikleri ve farklılıkları gösteren genetik hastalıklar arasında yer almaktadır. Down sendromlu veya otistik çocuklar beslenme açısından riskli bir gruptur. Bu araştırma Sakarya Özel Eğitim Kurumunda eğitim gören çocukların beslenme tarzları ve ebeveynlerin çocuk beslenmesine yönelik tutumlarının incelenmesi amacıyla planlanmıştır. 30 Gönüllü öğrenci velilerine demografik bilgiler haricinde evet/hayır içeren 17 (S1, S2,…,S17 soru sorulmuştur. Ayrıca okul çağında sıkça kullanılan 22 adet gıda maddesi tablosundaki yiyeceklerin sıklığı sorgulanmıştır. S4 (%45,2 evet ve %54,8 hayır, S8 (%41,9 evet ve %54,8 hayır, S15 (%32,3 evet ve %67,7 hayır ve S16’ da ise (%3,2 evet ve %96,8 hayır hayır cevaplarının oranı evet cevabına göre yüksek bulunurken diğer soruların cevaplarında evet oranı yüksek bulunmuştur. Öğün aralarında çocuğun isteklerinin karşılanmaması, çocuğun acıkınca yemek yedirilmemesi, çocuğun her gün düzenli olarak akşam yemeği yememesi aile bireylerinin çocuk besleme bilgisinde yetersiz olduğu düşünülmektedir. Bununla birlikte otistik çocukların cips, gazlı içecekler, lahmacun-pide türü gıdalara az yer verilmesi ve süt ve süt ürünleri, yumurta, kuru baklagiller ekmek ve makarna gibi yiyeceklere yönelmesi olumlu bir beslenme alışkanlığını göstermektedir.

Integrated adsorptive technique for efficient recovery of m-cresol and m-toluidine from actual acidic and salty wastewater

International Nuclear Information System (INIS)

Chen, Da; Liu, Fuqiang; Zong, Lidan; Sun, Xiaowen; Zhang, Xiaopeng; Zhu, Changqing; Tao, Xuewen; Li, Aimin

2016-01-01

Highlights: • An integrated adsorptive technique was designed. • Most resources could be recovered efficiently. • Reagents consumption decreased sharply. • Zero emission might be realized with the integrated technique. - Abstract: An integrated adsorptive technique combining an m-cresol adsorption unit, an acid retardation unit and an m-toluidine adsorption unit in sequence was designed to recover m-cresol and m-toluidine from highly acidic and salty m-cresol manufacturing wastewater. In the first column packed with hypercrosslinked polymeric resin (NDA-99), most m-cresol was captured through π-π and hydrogen-bonding interactions as well as the salting-out effect, while m-toluidine was not absorbed due to protonation. To separate acid from salt, an acid retardation unit was introduced successively to adsorb sulfuric acid by strong base anion exchange resin (201 × 7). After the acid retardation unit and mild neutralization reaction, the last column filled with NDA-99 was applied to trap neutral m-toluidine from the salty effluent. Moreover, the eluent of the acid retardation unit was utilized as the regenerant to recover m-toluidine, and the recycled high-acidity and low-salinity solution of m-toluidine was directly used to produce m-cresol as the raw material. Therefore, the proposed method not only efficiently recycled m-cresol and m-toluidine, but also reduced the consumption of alkali dramatically (saving 0.1628 t/t wastewater). These findings will inspire design of integrated adsorptive techniques for treating complex organic wastewater with high efficiency and low cost.

Integrated adsorptive technique for efficient recovery of m-cresol and m-toluidine from actual acidic and salty wastewater

Energy Technology Data Exchange (ETDEWEB)

Chen, Da [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Liu, Fuqiang, E-mail: jogia@163.com [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); State Environmental Protection Engineering Center for Organic Chemical Industrial Waste Water Disposal Resource Reuse, Nanjing 210046 (China); Zong, Lidan; Sun, Xiaowen; Zhang, Xiaopeng; Zhu, Changqing; Tao, Xuewen [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); Li, Aimin [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046 (China); State Environmental Protection Engineering Center for Organic Chemical Industrial Waste Water Disposal Resource Reuse, Nanjing 210046 (China)

2016-07-15

Highlights: • An integrated adsorptive technique was designed. • Most resources could be recovered efficiently. • Reagents consumption decreased sharply. • Zero emission might be realized with the integrated technique. - Abstract: An integrated adsorptive technique combining an m-cresol adsorption unit, an acid retardation unit and an m-toluidine adsorption unit in sequence was designed to recover m-cresol and m-toluidine from highly acidic and salty m-cresol manufacturing wastewater. In the first column packed with hypercrosslinked polymeric resin (NDA-99), most m-cresol was captured through π-π and hydrogen-bonding interactions as well as the salting-out effect, while m-toluidine was not absorbed due to protonation. To separate acid from salt, an acid retardation unit was introduced successively to adsorb sulfuric acid by strong base anion exchange resin (201 × 7). After the acid retardation unit and mild neutralization reaction, the last column filled with NDA-99 was applied to trap neutral m-toluidine from the salty effluent. Moreover, the eluent of the acid retardation unit was utilized as the regenerant to recover m-toluidine, and the recycled high-acidity and low-salinity solution of m-toluidine was directly used to produce m-cresol as the raw material. Therefore, the proposed method not only efficiently recycled m-cresol and m-toluidine, but also reduced the consumption of alkali dramatically (saving 0.1628 t/t wastewater). These findings will inspire design of integrated adsorptive techniques for treating complex organic wastewater with high efficiency and low cost.

Damla Sulama Koşullarında Aşılı ve Aşısız Karpuzlarda Bitki, Su ve Verim İlişkilerinin İrdelenmesi

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Selçuk ÖZMEN

2014-09-01

Full Text Available Bu çalışma, 2006-2008 yılları arasında Çukurova Üniversitesi, Ziraat Fakültesi, Tarımsal Yapılar ve Sulama Bölümü deneme alanında yürütülmüştür. Araştırmada, damla sulama koşullarında aşılı ve aşısız karpuzlarda bitki, su ve verim ilişkileri irdelenmiştir. Denemede; tam sulanan aşılı (Crimson Tide+Jumbo:CTJ ve aşısız (Crimson Tide: CT konuları ele alınmıştır. Sulama suyu, sulama aralıkları arasında oluşan toplam A Sınıfı buharlaşma kabından okunan değerlere göre hesaplanmıştır. Çalışmada, konulara göre, sulama suyu (IW ve bitki su tüketimleri (ET, sırasıyla, 266.2–413.5 mm (12–16 sulama ve 433.1-520.6 mm arasında değişmiş; en yüksek IW ve mevsimlik ET ise, sırasıyla, 413.5 mm ve 520.6 mm ile CTJ konusundan 2006 yılında elde edilmiştir. Karpuz bitkisinde en yüksek ortalama aylık ET, 214.0 mm ile CTJ konusunda Mayıs ayında ölçülmüş; bu ayda ortalama çim kıyas bitki su tüketimi (ETo, 163.1 mm olarak belirlenmiştir. Bitki katsayısı (Kc değerlerinin; deneme yılları için, başlangıç, mevsim ortası ve mevsim sonudönemlerinde, sırasıyla, 0.240.67, 0.87-1.66 ve 0.83-0.93 olarak değiştiği saptanmıştır. Son yılki çalışmada; toplam kök yoğunluğunun aşılı konularda aşısızlara kıyasla % 4 daha fazla; yaprak su potansiyeli (YSP değerlerlerinin -0.20 ile -0.70 MPa arasında değiştiği ve aşılı konuların daha yüksek YSP değerlerine sahip olduğu saptanmıştır. Aşılama ile verim, toplam su kullanma randımanı (TWUE ve su kullanım randımanı (IWUE artarken sulama suyunun ET içerisindeki kullanım oranı (IWc aşısız konularında daha yüksek olduğu belirlenmiştir. En yüksek verim 712.8 kg/ha ile CTJ konusundan 2008 yılından elde edilmiştir

Two new coordination polymers constructed by naphthalene-1,4-dicarboxylic acid and 2,4-diamino-6-methyl-triazine

Energy Technology Data Exchange (ETDEWEB)

Li, Yamin, E-mail: liyamin@henu.edu.cn [Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004 (China); Xiao, Changyu [Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004 (China); Zhang, Xudong [State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China); Xu, Yanhui [Department of Medical Imaging, Bethune Medical Non-Commissioned Officer' s, College, Shijiazhuang, Hebei 050081 (China); Li, Junrui; Lun, Huijie; Chen, Qi [Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004 (China)

2013-08-15

Two new transition metal coordination complexes, ([MnO(nda)](H{sub 2}dmt)(H{sub 2}O)){sub n} (1), [Ag{sub 5}(nda){sub 2.5}(dmt)]{sub n} (2), (H{sub 2}nda=naphthalene-1,4-dicarboxylic acid, dmt=2,4-diamine-6-methyl-1,3,5-triazine) have been hydrothermally synthesized by the reactions of H{sub 2}nda and dmt with the homologous MnCl{sub 2}·4H{sub 2}O and AgNO{sub 3}, respectively, and characterized by single-crystal X-ray diffraction, IR spectra, elemental analysis, thermogravimetric analysis (TGA). The compound 1 exhibits a 3D network comprising 1D metal chain (MnO(CO{sub 2}){sub 2}){sub n} connected by the ligand nda{sup 2−}, featuring a four-connected uninodal diamond -like topology. In compound 2, it is firstly observed that decanuclear silver units as secondary building units to construct 3D network by the ligands dmt and nda{sup 2−}, with a rare 2-nodal (3,8)-connected tfz-d topology ((4{sup 3}){sub 2}(4{sup 6}.6{sup 18}.8{sup 4})). The interactions within each Mn(II)—Mn(II) pair of compound 1 are antiferromagnetic (g=2.07, J=−1.42(1) cm{sup −1}, zj′=−0.73(2) cm{sup −1}). In addition, compound 2 exhibits photoluminescent property at about 472 nm (λ{sub ex}=394 nm). - Graphical abstract: Two new transition metal coordination complexes 1–2 have been hydrothermally synthesized and characterized by single-crystal X-ray diffraction, IR spectra, elemental analysis thermogravimetric analysis (TGA). Highlights: • The compound 1 exhibits a 3D network with four-connected uninodal diamond-like topology. • The first 3D network of 2 with a rare tfz-d topology consists of decanuclear silver clusters as secondary building units. • The magnetic measurement indicates the compound 1 shows antiferromagnetic interactions. • The photoluminescent property of 2 has been measured.

Tam Zamanında Üretim Sistemlerinde Hata Önleyiciler: Poka-

OpenAIRE

BAY, Murat; ÇİÇEK, Ercan

2007-01-01

Günümüzde, birçok işletme yalın üretime üretim performansının artması için geçmektedir. İstatistiksel proses kontrol gibi araçlar hatayı göstermekte, ancak önlememektedir. Poka-Yoke’nin altında yatan temel düşünce ise, çalışanların dikkatsizliklerini önlemektir. Bu çalışmada, poka-yoke sistemleri özellikle kurulum ve kullanım maliyetinin düşük olması ve maliyet kazancı olduğu için incelenmiştir

Barış Harekâtı Sonrasında Adana’dan Kıbrıs’a Gerçekleştirilen Nüfus Nakli (1975-1976)

OpenAIRE

ÇANAK, Erdem

2015-01-01

1571-1878 yılları arasında Osmanlı Devleti’nin hâkimiyeti altında bulunan Kıbrıs, 1878 yılında geçici olarak, Lozan Antlaşması ile de resmen İngiltere’ye geçmiştir. Bu durumdan faydalanmak isteyen Rumlar da “Enosis”e yönelik faaliyetlerine hız vermişlerdir. Adadaki Türklerin var olma mücadelesine neden olan bu süreç, 1974 Barış Harekâtı ile neticelenmiştir. Ancak Türkler, Barış Harekâtı’na kadar olan dönemde maruz kaldıkları baskıdan dolayı zaman zaman başta Türkiye olmak üzere dünyanın farkl...

Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination

International Nuclear Information System (INIS)

Ballesta-Claver, J.; Rodríguez-Gómez, R.; Capitán-Vallvey, L.F.

2013-01-01

Highlights: ► Cathodic ECL offers conventional and non-aggressive analysis conditions. ► The ECL hydrogen peroxide/ruthenium complex system for uric acid determination is novel. ► The ruthenium complex is electrochemically immobilized on graphite screen-printed electrodes. ► The quantification of the uric acid is based on a Stern–Volmer type equation. ► The use of the cathodic ECL working methodology reduces interferences during analysis. -- Abstract: A new method for uric acid (UA) determination based on the quenching of the cathodic ECL of the tris(2,2-bipyridine)ruthenium(II)–uricase system is described. The biosensor is based on a double-layer design containing first tris(2,2-bipyridine)ruthenium(II) (Ru(bpy) 3 2+ ) electrochemically immobilized on graphite screen-printed cells and uricase in chitosan as a second layer. The uric acid biosensing is based on the ECL quenching produced by uric acid over the cathodic ECL caused by immobilized Ru(bpy) 3 2+ in the presence of uricase. The use of a −1.1 V pulse for 1 s with a dwelling time of 10 s makes it possible to estimate the initial enzymatic rate, which is used as the analytical signal. The Stern–Volmer type calibration function shows a dynamic range from 1.0 × 10 −5 to 1.0 × 10 −3 M with a limit of detection of 3.1 × 10 −6 M and an accuracy of 13.6% (1.0 × 10 −4 M, n = 5) as relative standard deviation. Satisfactory results were obtained for urine samples, creating an affordable alternative for uric acid determination

Comparative Study of Different Nano-Formulations of Curcumin for Reversal of Doxorubicin Resistance in K562R Cells.

Science.gov (United States)

Dash, Tapan K; Konkimalla, V Badireenath

2017-02-01

Curcumin is very well established as a chemo-therapeutic, chemo-preventive and chemo-sensitizing agent in diverse disease conditions. As the isolated pure form has poor solubility and pharmacokinetic problems, therefore it is encapsulated in to several nano-formulations to improve its bioavailability. Here in the current study, we aim to compare different nano-formulations of curcumin for their chemo-sensitizing activity in doxorubicin (DOX) resistant K562 cells. Four different curcumin formulations were prepared namely DMSO assisted curcumin nano-dispersion (CurD, 260 nm), liposomal curcumin (CurL, 165 nm), MPEG-PCL micellar curcumin (CurM, 18 nm) and cyclodextrin encapsulated curcumin (CurN, 37 nm). The formulations were subjected to particle characterizations (size, zeta potential, release studies), followed by biological assays such as cellular uptake, P-gp inhibitory activity and reversal of DOX resistance by co-treatment with DOX. Curcumin uptake in K562N and K562R cells was mildly reduced when treated with CurL and CurM, while for CurD and CurN the uptake remained equivalent. However, CurL retained P-gp inhibitory activity of curcumin and with a considerable chemo-sensitizing effect but CurM showed no P-gp inhibitory activity. CurN retained above biological activities, but requires a secondary carrier under in vivo conditions. From the results, CurM was found to be most suitable for solubilization of curcumin where as CurL can be considered as most suitable nano-formulation for reversal of DOX resistance.

Vişne ve Nar Suyu ve Konsantratlarında Antosiyaninlerin Degradasyonu

Directory of Open Access Journals (Sweden)

Narmela Asafı

2015-02-01

Full Text Available Nar ve vişne suyu konsantratlarında antosiyaninlerin parçalanması üzerine sıcaklığın etkisi incelenmiştir. 70°Bx'lik konsantratlardan 45°Bx ve 15° Bx lik örnekler hazırlanarak -18°C, 5°C, 20°C ve 37°C depolanmıştır. Depolanma süresince, sıcaklık dercesine bağlı olarak değişik aralıklarla alman örneklerde antosiyanin kaybı saptanarak parçalanma hızı incelenmiştir. Elde edilen bulgulara göre antosiyaninlerin parçalanma­sı birinci dereceden bir reaksiyon kinetiğine göre gelişmektedir. Tüm örneklerde beklendiği gibi sıcaklık derecesi yükseldikçe antosiyaninlerin degradasyon hızları artmaktadır. Örneğin 15° Bx'lik vişne suyunda -18°C'de hız konsantı k=0.534 x 10-3 gün-1 olduğu halde, 37°C'de k= 184 x 10-3 gün-1 'e yükselmiştir. Aynı şekilde 15° Bx'lik vişne suyunda -18°C'de hız konstantı k= 0.203 x 10-3 gün-1 olduğu halde 37°C'de k= 94 x10-3 gün-1'e yükselmiştir. Ayrıca reaksiyonun sıcaklığa bağımlılığı, Arrhenius eşitliğinden yararlanarak açıklanmaya çalışılmıştır. Nar suyu ve konsantratlannda antosiyaninlerin degradasyon hızı, vişne suyu ve konsantratlarında olduğundan daha yüksektir. Böylece nar suyu ve konsantratlarında depolama süresince sıcaklığa bağlı olarak hızla renk kaybı oluşmaktadır. Ayrıca doğal briksindeki vişne suyunda antosiyaninler, konsantratlarda olduğundan daha stabil olduğu halde nar suyunda bunun aksine konsantratlardaki antosiyaninlerin daha stabil ol­duğu anlaşılmaktadır.

Maternal Docosahexaenoic Acid Intake Levels during Pregnancy and Infant Performance on a Novel Object Search Task at 22 Months

Science.gov (United States)

Rees, Alison; Sirois, Sylvain; Wearden, Alison

2014-01-01

This study investigated maternal prenatal docosahexaenoic acid (DHA) intake and infant cognitive development at 22 months. Estimates for second- and third-trimester maternal DHA intake levels were obtained using a comprehensive Food Frequency Questionnaire. Infants (n = 67) were assessed at 22 months on a novel object search task. Mothers'…

Detection of pyrrolizidine alkaloids using flow analysis with both acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence

Energy Technology Data Exchange (ETDEWEB)

Gorman, Bree A. [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia); Barnett, Neil W. [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia)]. E-mail: barnie@deakin.edu.au; Bos, Richard [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia)

2005-06-13

For the first time, analytically useful chemiluminescence was elicited from the reactions of the pyrrolizidine alkaloids. Heliotrine, retronecine, supinine, monocrotaline and echinatine N-oxide yielded chemiluminescence upon reaction with tris(2,2'-bipyridyl)ruthenium(II) whilst lasiocarpine, its N-oxide and supinine elicited light upon reaction with acidic potassium permanganate. Detection limits for heliotrine were 1.25 x 10{sup -7} M and 9 x 10{sup -9} M for tris(2,2'-bipyridyl)ruthenium(III) perchlorate with flow injection analysis (FIA) and the silica-immobilised reagent (4-[4-(dichloromethylsilanyl)-butyl]-4'-methyl-2,2'-bipyridine)bis (2,2'-bipyridyl)ruthenium(II) with sequential injection analysis (SIA), respectively. Lasiocarpine was detectable at 1.4 x 10{sup -7} M using acidic potassium permanganate with FIA. Additionally, the silica-immobilised reagent was optimised with respect to the oxidant (ammonium ceric nitrate) concentration and the aspiration times which afforded a detection limit for codeine of 5 x 10{sup -10} M using SIA.

Tıbbi Adaçayı (Salvia officinalis L.’nda Farklı Hasat Zamanlarının Uçucu Yağ ve Fenolik Bileşikler ile Antioksidan Aktivite Üzerine Etkisi

Directory of Open Access Journals (Sweden)

Müge BAŞYİĞİT

2016-11-01

Full Text Available Bu araştırma, bir yıl süreyle 12 ayı temsil edecek şekilde farklı zamanlarda hasat edilen tıbbi adaçayı (Salvia officinalis L.’nın herba ve yaprak verimi, yaprakların taşıdığı uçucu yağ oranı ve kompozisyonu, ekstrede  Folin-Ciocalteu kolorimetrik metodu kullanılarak toplam fenolik madde miktarı, fenolik bileşikleri, serbest radikal süpürücü aktivitesi (DPPH ve demir iyonu indirgeme gücü (FRAP yöntemlerine göre antioksidan aktivitelerini belirlemek amacıyla yapılmıştır. Tıbbi adaçayında taze herba verimi 500.0-961.0 kg/da, kuru herba verimi 223.4-556.6 kg/da, kuru yaprak verimi 129.6-367.2 kg/da, uçucu yağ oranı %0.83-3.33, toplam fenolik madde miktarı 14.54-30.83 mg/g arasında değişim göstermiştir. Yaz ve güz aylarında hasat edilen bitkilerin herba ve yaprak verimleri, uçucu yağ oranları ve verimleri ile toplam fenolik madde miktarları kış ve bahar aylarında hasat edilen bitkilere göre daha yüksek bulunmuştur. Tıbbi adaçayının uçucu yağ kompozisyonunu oluşturan en önemli bileşenlerin 1,8-sineol (%11.93-31.87, α-tuyon (%15.72-26.26, β-tuyon (%4.51-27.67 ve kamfor (%3.65-23.02 olduğu, 1,8-sineol ve kamfor oranları ilkbahar aylarında daha düşük oranlarda, α- ve β-tuyon oranları ise ilkbahar aylarında daha yüksek oranlarda bulunduğu tespit edilmiştir. Tıbbi adaçayının en önemli fenolik bileşenlerinin ise rosmarinik asit (15.15-100.57 mg/g, naringin (9.59-41.81 mg/g, hesperidin (9.80-53.26 mg/g ve rutin (0.73-10.04 mg/g olduğu, en yüksek antioksidan aktivite Mayıs ve Haziran aylarında biçilen ve en düşük antioksidan aktivite ise Mart ve Nisan aylarında biçilen tıbbi adaçayı yapraklarında gözlenmiştir.

Bioassay studies of metal(II complexes of 2,2'-(ethane-1,2-diyldiiminodiacetic acid

Directory of Open Access Journals (Sweden)

Saeed-ur-Rehman

2011-08-01

Full Text Available Ni(II, Cu(II and Zn(II coordination compounds with modified diammine 2,2'-(ethane-1,2-diyldiiminodiacetic acid (EDDA were prepared and characterized. Coordination complexes of the EDDA were characterized by physical measurements including elemental analysis, IR, UV-Visible, magnetic susceptibilities and conductance measurements. The complexes were screened against four pathogenic bacteria like Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus and their concentrations for maximum inhibition zones were obtained.

Yeşilırmak Nehir Teraslarında Toprakların Oluşumu ve Sınıflandırılması

Directory of Open Access Journals (Sweden)

Alper Durak

2014-02-01

Full Text Available Bu çalışmada ana materyali alüviyaller olan, Yeşilırmak nehir terasları üzerinde oluşmuş topraklar incelenmiştir. Bu amaçla çalışma alanında 4 farklı profil açılmıştır. Açılan profillerden horizon esasına göre toplam 27 adet örnek alınmış ve laboratuvarda fiziksel ve kimyasal analizleri yapılmıştır. Tanımlanan profiller Toprak Taksonomisi’ne göre, Alt grup düzeyinde; Kum Ocağı ve Çakıl profilleri Typic Ustipsamments, Havaalanı profili Typic Haplustolls ve Mera profili ise Typic Argiustolls olarak sınıflandırılmıştır. Toprakların pH değerleri 7,38-8,41 arasında değişmekte olup, topraklar hafif ve kuvvetli bazik reaksiyonlu olarak sınıflandırılmıştır. Toprakların çoğunda, pH değerleri profil içinde düzensiz olarak dağılmıştır. Bir kısmında is pH değerlerinde derinliğe bağlı olarak artış görülmüştür. Çalışma alanı topraklarında kireç %0,8-25,4 arasında değişmiştir. Toprakların kireç içerikleri geniş sınırlar içinde değişmesine rağmen, büyük çoğunluğu fazla kireçli olarak sınıflandırılmıştır. Toprakların önemli bir kısmında, kireç profil içinde düzensiz olarak dağılmıştır. Topraklarda organik madde içeriği %0,14-6,11 arasındadır. Toprakların organik madde içerikleri geniş sınırlar içerisinde değişim göstermesine rağmen, büyük çoğunluğunun organik madde içeriği düşük düzeydedir. Organik madde içeriği sadece iki profilin yüzey horizonlarında %4’ün üzerindedir. Çalışma sonunda alüviyal ana materyal, tekstür, topoğrafya ve zamanın; tanımlanan toprakların oluşumunu farklı derecede etkilediği ortaya konmuştur.

PaDef defensin from avocado (Persea americana var. drymifolia) is cytotoxic to K562 chronic myeloid leukemia cells through extrinsic apoptosis.

Science.gov (United States)

Flores-Alvarez, Luis José; Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Salgado-Garciglia, Rafael; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

2018-06-01

Plant defensins, a group of antimicrobial peptides, show selective cytotoxicity toward cancer cells. However, their mechanisms of action remain poorly understood. Here, we evaluated the cytotoxicity of PaDef defensin from avocado (Persea americana var. drymifolia) on K562 chronic myeloid leukemia cells and analyzed the pathway involved in the induction of cell death. The defensin PaDef was not cytotoxic against human PBMCs; however, it was cytotoxic for K562 cell line (IC 50  = 97.3 μg/ml) activating apoptosis at 12 h. PaDef did not affect the mitochondrial membrane potential (ΔΨm), neither the transmembranal potential or the release of intracellular calcium. Also, PaDef induced gene expression of caspase 8 (∼2 fold), TNF-α (∼4 fold) and TNFR1 (∼10 fold). In addition, the activation of caspase 8 was detected at 24 h, whereas caspase 9 activity was not modified, suggesting that the extrinsic apoptosis pathway could be activated. In conclusion, PaDef induces apoptosis on K562 cells, which is related to the activation of caspase 8 and involves the participation of TNF-α, which is a novel property for a plant defensin. Copyright © 2018 Elsevier Ltd. All rights reserved.

NECSA'S Need to Establish a Nuclear Forensics Specific NDA Facility for On-Site Categorization of Seized Nuclear Materials

International Nuclear Information System (INIS)

Boshielo, P.; Mogafe, R.

2015-01-01

The increase of nuclear material that are out of regulatory control is becoming a serious concern and threat and thereby continuously seeking urgent interventions and counteractions from the international community aspiring effective control over all nuclear material and peaceful uses of nuclear technologies globally. In South Africa the nuclear forensics initiative approach and its execution have been adopted, established and managed by the South African Nuclear Energy Corporation (NECSA) to support the country's nuclear safeguards system and nuclear security investigations plan to fight against the illicit trafficking of nuclear and radioactive materials. On this nuclear forensics initiative approach adopted by Necsa, the development and later execution of a Non-Destructive Analyses (NDA) facility capability for quick categorization of any seized nuclear material by law-enforcement agencies is currently envisaged as a critical initiative to comprehend nuclear forensics Laboratory analytical or characterization techniques. The main objective for this NDA facility is planned to be used for performing nuclear material screening process for material categorization purposes to generate information and results which will be open to law enforcement agencies for prosecution processes and also for the safeguards reporting to the IAEA (ITDB). The NDA technique is therefore found to be a critical tool needed at NECSA as an Early-Checking-Point or first-line material check point for all seized nuclear materials in determining some characteristics of the materials and collection of data without having to destroy or changing the morphology of the material. (author)

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to a combination of plant sterols and Cholesternorm®mix and reduction of blood LDL-cholesterol concentrations pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Health Concern B.V., submitted for authorisation of a claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of the Netherlands, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the sci......Following an application from Health Concern B.V., submitted for authorisation of a claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of the Netherlands, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion...... on the scientific substantiation of a health claim related to a combination of plant sterols and Cholesternorm®mix and reduction of blood LDL-cholesterol concentrations. The food which is the subject of the health claim is a combination of plant sterols (free and in esterified form) and Cholesternorm......®mix and provides at the levels of the proposed conditions of use around 0.52 g plant sterols, 0.95 g linoleic acid, 0.13 g alpha-linolenic acid and 0.13 g pectins per day. The combination of plant sterols and Cholesternorm®mix, which is the subject of the claim, is sufficiently characterised in relation...

Unravelling pathways downstream Sox6 induction in K562 erythroid cells by proteomic analysis

KAUST Repository

Barbarani, Gloria

2017-10-20

The Sox6 transcription factor is crucial for terminal maturation of definitive red blood cells. Sox6-null mouse fetuses present misshapen and nucleated erythrocytes, due to impaired actin assembly and cytoskeleton stability. These defects are accompanied with a reduced survival of Sox6-/- red blood cells, resulting in a compensated anemia. Sox6-overexpression in K562 cells and in human primary ex vivo erythroid cultures enhances erythroid differentiation and leads to hemoglobinization, the hallmark of erythroid maturation. To obtain an overview on processes downstream to Sox6 expression, we performed a differential proteomic analysis on human erythroid K562 cells overexpressing Sox6. Sox6-overexpression induces dysregulation of 64 proteins, involved in cytoskeleton remodeling and in protein synthesis, folding and trafficking, key processes for erythroid maturation. Moreover, 43 out of 64 genes encoding for differentially expressed proteins contain within their proximal regulatory regions sites that are bound by SOX6 according to ENCODE ChIP-seq datasets and are possible direct SOX6 targets. SAR1B, one of the most induced proteins upon Sox6 overexpression, shares a conserved regulatory module, composed by a double SOX6 binding site and a GATA1 consensus, with the adjacent SEC24 A gene. Since both genes encode for COPII components, this element could concur to the coordinated expression of these proteins during erythropoiesis.

Synthesis, characterization and crystal structure of (2RS,4R)-2-(2-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid

Science.gov (United States)

Muche, Simon; Müller, Matthias; Hołyńska, Małgorzata

2018-03-01

The condensation reaction of ortho-vanillin and L-cysteine leads to formation of a racemic mixture of (2RS,4R)-2-(2-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid and not, as reported in the available literature, to a Schiff base. The racemic mixture was fully characterized by 1D and 2D NMR techniques, ESI-MS and X-ray diffraction. Addition of ZnCl2 led to formation of crystals in form of colorless needles, suitable for X-ray diffraction studies. The measured crystals were identified as the diastereomer (2R,4R)-2-(2-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid 1. The bulk material is racemic. Thiazolidine exists as zwitterion in solid state, as indicated by the crystal structure.

Effect of 5-aminolevulinic acid on erythropoiesis: A preclinical in vitro characterization for the treatment of congenital sideroblastic anemia

International Nuclear Information System (INIS)

Fujiwara, Tohru; Okamoto, Koji; Niikuni, Ryoyu; Takahashi, Kiwamu; Okitsu, Yoko; Fukuhara, Noriko; Onishi, Yasushi; Ishizawa, Kenichi; Ichinohasama, Ryo; Nakamura, Yukio; Nakajima, Motowo; Tanaka, Tohru; Harigae, Hideo

2014-01-01

Highlights: • Treatment with ALA induces erythroid differentiation of K562 cells. • Transportation of ALA into erythroid cells occurs predominantly via SLC36A1. • ALA restores defects in ALAS2 in human iPS cell-derived erythroblasts. • ALA may represent a novel therapeutic option for CSA caused by ALAS2 mutations. - Abstract: Congenital sideroblastic anemia (CSA) is a hereditary disorder characterized by microcytic anemia and bone marrow sideroblasts. The most common form of CSA is attributed to mutations in the X-linked gene 5-aminolevulinic acid synthase 2 (ALAS2). ALAS2 is a mitochondrial enzyme, which utilizes glycine and succinyl-CoA to form 5-aminolevulinic acid (ALA), a crucial precursor in heme synthesis. Therefore, ALA supplementation could be an effective therapeutic strategy to restore heme synthesis in CSA caused by ALAS2 defects. In a preclinical study, we examined the effects of ALA in human erythroid cells, including K562 cells and human induced pluripotent stem cell-derived erythroid progenitor (HiDEP) cells. ALA treatment resulted in significant dose-dependent accumulation of heme in the K562 cell line. Concomitantly, the treatment substantially induced erythroid differentiation as assessed using benzidine staining. Quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis confirmed significant upregulation of heme-regulated genes, such as the globin genes [hemoglobin alpha (HBA) and hemoglobin gamma (HBG)] and the heme oxygenase 1 (HMOX1) gene, in K562 cells. Next, to investigate the mechanism by which ALA is transported into erythroid cells, quantitative RT-PCR analysis was performed on previously identified ALA transporters, including solute carrier family 15 (oligopeptide transporter), member (SLC15A) 1, SLC15A2, solute carrier family 36 (proton/amino acid symporter), member (SLC36A1), and solute carrier family 6 (neurotransmitter transporter), member 13 (SLC6A13). Our analysis revealed that SLC36A1 was abundantly

Effect of 5-aminolevulinic acid on erythropoiesis: A preclinical in vitro characterization for the treatment of congenital sideroblastic anemia

Energy Technology Data Exchange (ETDEWEB)

Fujiwara, Tohru [Department of Hematology and Rheumatology, Tohoku University Graduate School, Sendai (Japan); Department of Molecular Hematology/Oncology, Tohoku University Graduate School, Sendai (Japan); Okamoto, Koji; Niikuni, Ryoyu [Department of Hematology and Rheumatology, Tohoku University Graduate School, Sendai (Japan); Takahashi, Kiwamu [SBI Pharmaceuticals Co., Ltd., Tokyo (Japan); Okitsu, Yoko; Fukuhara, Noriko; Onishi, Yasushi [Department of Hematology and Rheumatology, Tohoku University Graduate School, Sendai (Japan); Ishizawa, Kenichi [Department of Hematology and Rheumatology, Tohoku University Graduate School, Sendai (Japan); Clinical Research, Innovation and Education Center, Tohoku University Hospital, Sendai (Japan); Ichinohasama, Ryo [Department of Hematopathology, Tohoku University Graduate School, Sendai (Japan); Nakamura, Yukio [Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki (Japan); Nakajima, Motowo; Tanaka, Tohru [SBI Pharmaceuticals Co., Ltd., Tokyo (Japan); Harigae, Hideo, E-mail: harigae@med.tohoku.ac.jp [Department of Hematology and Rheumatology, Tohoku University Graduate School, Sendai (Japan); Department of Molecular Hematology/Oncology, Tohoku University Graduate School, Sendai (Japan)

2014-11-07

Highlights: • Treatment with ALA induces erythroid differentiation of K562 cells. • Transportation of ALA into erythroid cells occurs predominantly via SLC36A1. • ALA restores defects in ALAS2 in human iPS cell-derived erythroblasts. • ALA may represent a novel therapeutic option for CSA caused by ALAS2 mutations. - Abstract: Congenital sideroblastic anemia (CSA) is a hereditary disorder characterized by microcytic anemia and bone marrow sideroblasts. The most common form of CSA is attributed to mutations in the X-linked gene 5-aminolevulinic acid synthase 2 (ALAS2). ALAS2 is a mitochondrial enzyme, which utilizes glycine and succinyl-CoA to form 5-aminolevulinic acid (ALA), a crucial precursor in heme synthesis. Therefore, ALA supplementation could be an effective therapeutic strategy to restore heme synthesis in CSA caused by ALAS2 defects. In a preclinical study, we examined the effects of ALA in human erythroid cells, including K562 cells and human induced pluripotent stem cell-derived erythroid progenitor (HiDEP) cells. ALA treatment resulted in significant dose-dependent accumulation of heme in the K562 cell line. Concomitantly, the treatment substantially induced erythroid differentiation as assessed using benzidine staining. Quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis confirmed significant upregulation of heme-regulated genes, such as the globin genes [hemoglobin alpha (HBA) and hemoglobin gamma (HBG)] and the heme oxygenase 1 (HMOX1) gene, in K562 cells. Next, to investigate the mechanism by which ALA is transported into erythroid cells, quantitative RT-PCR analysis was performed on previously identified ALA transporters, including solute carrier family 15 (oligopeptide transporter), member (SLC15A) 1, SLC15A2, solute carrier family 36 (proton/amino acid symporter), member (SLC36A1), and solute carrier family 6 (neurotransmitter transporter), member 13 (SLC6A13). Our analysis revealed that SLC36A1 was abundantly

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the extension of use for DHA and EPA-rich algal oil from Schizochytrium sp. as a Novel Food ingredient

DEFF Research Database (Denmark)

Tetens, Inge; Poulsen, Morten

2014-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on an extension of use for docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)-rich algal oil from Schizochytrium sp. as a novel food...... population, excluding pregnant and lactating women. In a previous opinion on the Tolerable Upper Intake Level of EPA, DHA and docosapentaenoic acid (DPA), the Panel concluded that supplemental intake of EPA and DHA combined at doses up to 5 g/day, does not give rise to safety concerns for adults. Based...... ingredient (NFI) in the context of Regulation (EC) No 258/97. The NFI is already authorised for use in a range of foodstuffs at specified maximum levels. The applicant requests an extension of use of the NFI in food supplements up to a maximum DHA and EPA content of 3 g per daily dose for the adult...

Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination

Energy Technology Data Exchange (ETDEWEB)

Ballesta-Claver, J.; Rodríguez-Gómez, R. [ECsens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, E-18071 Granada (Spain); Capitán-Vallvey, L.F., E-mail: lcapitan@ugr.es [ECsens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, E-18071 Granada (Spain)

2013-04-03

Highlights: ► Cathodic ECL offers conventional and non-aggressive analysis conditions. ► The ECL hydrogen peroxide/ruthenium complex system for uric acid determination is novel. ► The ruthenium complex is electrochemically immobilized on graphite screen-printed electrodes. ► The quantification of the uric acid is based on a Stern–Volmer type equation. ► The use of the cathodic ECL working methodology reduces interferences during analysis. -- Abstract: A new method for uric acid (UA) determination based on the quenching of the cathodic ECL of the tris(2,2-bipyridine)ruthenium(II)–uricase system is described. The biosensor is based on a double-layer design containing first tris(2,2-bipyridine)ruthenium(II) (Ru(bpy){sub 3}{sup 2+}) electrochemically immobilized on graphite screen-printed cells and uricase in chitosan as a second layer. The uric acid biosensing is based on the ECL quenching produced by uric acid over the cathodic ECL caused by immobilized Ru(bpy){sub 3}{sup 2+} in the presence of uricase. The use of a −1.1 V pulse for 1 s with a dwelling time of 10 s makes it possible to estimate the initial enzymatic rate, which is used as the analytical signal. The Stern–Volmer type calibration function shows a dynamic range from 1.0 × 10{sup −5} to 1.0 × 10{sup −3} M with a limit of detection of 3.1 × 10{sup −6} M and an accuracy of 13.6% (1.0 × 10{sup −4} M, n = 5) as relative standard deviation. Satisfactory results were obtained for urine samples, creating an affordable alternative for uric acid determination.

Waste characterization methods at belgoprocess and the importance of NDA

International Nuclear Information System (INIS)

Botte, J.; Luycx, P.

2003-01-01

Waste characterization in the end cycle becomes more and more important. Several methods are available for a radiological characterization: from copying the waste producers declaration over a calculation based on known characteristics or measured values to combinations of several techniques. The decision on what technique(s) to be used will be based on several criteria. One also has to evaluate at what stage of the waste treatment process the characterization has to be performed. Recently belgoprocess has performed large efforts and investments to assure a good waste characterization. These are concentrated in studies on historical and recent waste, resulting in isotopic vectors and the purchase of several NDA devices in order to cover the whole scala of waste the company treats. The measuring results always need to be integrated with isotopic vectors. (orig.)

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on Dietary Reference Values for carbohydrates and dietary fibre

DEFF Research Database (Denmark)

Tetens, Inge

This Opinion of the EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA) deals with the establishment of Dietary Reference Values for carbohydrates and dietary fibre. Nutritionally, two broad categories of carbohydrates can be differentiated: “glycaemic carbohydrates”, i.e. carbohydrates...

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on principles for deriving and applying Dietary Reference Values

DEFF Research Database (Denmark)

Tetens, Inge

This Opinion of the EFSA Panel on Dietetic products, Nutrition, and Allergies (NDA) deals with the general principles for development and application of Dietary Reference Values (DRVs). These quantitative reference values for nutrient intakes for healthy individuals and populations are based...

Dual Functions of the C5a Receptor as a Connector for the K562 Erythroblast-Like Cell-THP-1 Macrophage-Like Cell Island and as a Sensor for the Differentiation of the K562 Erythroblast-Like Cell during Haemin-Induced Erythropoiesis

Directory of Open Access Journals (Sweden)

Hiroshi Nishiura

2012-01-01

Full Text Available The transcriptional nuclear factor binding to the Y box of human leukocyte antigen genes (NF-Y for the C5a receptor (C5aR gene is active in erythroblasts. However, the roles of the C5aR in erythropoiesis are unclear. We have previously demonstrated that apoptotic cell-derived ribosomal protein S19 (RP S19 oligomers exhibit extraribosomal functions in promoting monocyte chemotaxis and proapoptosis via the C5aR without receptor internalisation. In contrast to the extraribosomal functions of the RP S19, a proapoptotic signal in pro-EBs, which is caused by mutations in the RP S19 gene, is associated with the inherited erythroblastopenia, Diamond-Blackfan anaemia. In this study, we detected C5aR expression and RP S19 oligomer generation in human erythroleukemia K562 cells during haemin-induced erythropoiesis. Under monocell culture conditions, the differentiation into K562 erythrocyte-like cells was enhanced following the overexpression of Wild-type RP S19. Conversely, the differentiation was repressed following the overexpression of mutant RP S19. An RP S19 oligomer inhibitor and a C5aR inhibitor blocked the association of the K562 basophilic EB-like cells and the THP-1 macrophage-like cells under coculture conditions. When bound to RP S19 oligomers, the C5aR may exhibit dual functions as a connector for the EB-macrophage island and as a sensor for EB differentiation in the bone marrow.

Farklı Durum Çeşitlerinden Mahalli ve Laboratuvar Koşullarında Yapılmış Bulguların Bazı Vitamin ve Mineral İçerikleri

Directory of Open Access Journals (Sweden)

Berrin Özkaya

2015-02-01

Full Text Available Araştırmada 5 farklı durum buğdayının, bunlardan mahalli ve laboratuvar koşullarında yapılan bulguların bazı kimyasal özellikleri ile tiamin, riboflavin ve mineral içerikleri karşılaştırılmıştır. Buğdayların yapımı sırasında buğdayların tiamin, özellikle de riboflavin miktarları azalmıştır. Mahalli koşullarda yapılan bulgurların tiamin ve riboflavin miktarları laboratuvarda yapılanlara kıyasla biraz düşük çıkmıştır. Bulgurların mineral içerikleri (Fe, Cu, Zn, Mn, Ca ve Mg miktarları yapıldıktan buğdaylara kıyasla daha düşük çıkmış, mahalli ve laboratuvar koşullarında yapılan bulgurların mineral madde miktarlarında önemli bir fark görülmemiştir.

Peyniraltı Suyu Tozundan Hazırlanan Sentetik Besi Ortamında Lactobacillus bulgaricus Suşlarının Oluşturduğu Laktik Asit Miktarı Üzerine bir Araştırma

OpenAIRE

Beyatlı, Yavuz; Tulumoğlu, Şener

1991-01-01

Peyniraltı suyu tozundan altı farklı sentetik besi ortamı hazırlanmıştır. Bu besi ortamlarında 12 adet L. bulgaricus suşunun oluşturduğu laktik asit miktarları tespit edilmiştir. Katkılı besi ortamlarında oluşan laktik asit miktarları, katkısız besi ortamları ile kıyaslandığında daha fazla bulunmuştur. 12 adet L. bulgaricus suşu arasında en fazla laktik asit üretenlerin L. bulgaricus B1, L. bulgaricus B4 ve L. bulgaricus B11 suşları olduğu görülmüştür.  

4-Methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylic Acid. Peculiarities of Preparation, Structure, and Biological Properties

Directory of Open Access Journals (Sweden)

Igor V. Ukrainets

2018-03-01

Full Text Available In order to determine the regularities of the structure–analgesic activity relationship, the peculiarities of obtaining, the spatial structure, and biological properties of 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylic acid and some of its derivatives have been studied. Using nuclear magnetic resonance (NMR spectroscopy and X-ray diffraction analysis, it has been proven that varying the reaction conditions using alkaline hydrolysis of methyl 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylate makes it possible to successfully synthesize a monohydrate of the target acid, its sodium salt, or 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine. The derivatographic study of the thermal stability of 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylic acid monohydrate has been carried out; based on this study, the optimal conditions completely eliminating the possibility of unwanted decomposition have been proposed for obtaining its anhydrous form. It has been shown that 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine is easily formed during the decarboxylation of not only 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylic acid, but also its sodium salt, which is capable of losing СО2 both in rather soft conditions of boiling in an aqueous solution, and in more rigid conditions of dry heating. The NMR spectra of the compounds synthesized are given; their spatial structure is discussed. To study the biological properties of 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylic acid and its sodium salt, the experimental model of inflammation caused by subplantar introduction of the carrageenan solution in one of the hind limbs of white rats was used. The anti-inflammatory activity and analgesic effect were assessed by the degree of edema reduction and the ability to affect the pain response compared to the animals of control groups. According to the results of the tests performed, it has been found that after intraperitoneal injection

c-myb stimulates cell growth by regulation of insulin-like growth factor (IGF) and IGF-binding protein-3 in K562 leukemia cells

Energy Technology Data Exchange (ETDEWEB)

Kim, Min-Sun; Kim, Sun-Young; Arunachalam, Sankarganesh [Department of Pediatrics, School of Medicine, Chonbuk National University, Jeonju 561-712 (Korea, Republic of); Hwang, Pyoung-Han [Department of Pediatrics, School of Medicine, Chonbuk National University, Jeonju 561-712 (Korea, Republic of); Research Institute of Clinical Medicine, School of Medicine, Chonbuk National University, Jeonju 561-712 (Korea, Republic of); Yi, Ho-Keun [Department of Biochemistry, School of Dentistry, Chonbuk National University, Jeonju 561-712 (Korea, Republic of); Nam, Sang-Yun [Department of Alternative Therapy, School of Alternative Medicine and Health Science, Jeonju University, Jeonju 561-712 (Korea, Republic of); Lee, Dae-Yeol, E-mail: leedy@chonbuk.ac.kr [Department of Pediatrics, School of Medicine, Chonbuk National University, Jeonju 561-712 (Korea, Republic of); Research Institute of Clinical Medicine, School of Medicine, Chonbuk National University, Jeonju 561-712 (Korea, Republic of)

2009-07-17

c-myb plays an important role in the regulation of cell growth and differentiation, and is highly expressed in immature hematopoietic cells. The human chronic myelogenous leukemia cell K562, highly expresses IGF-I, IGF-II, IGF-IR, and IGF-induced cellular proliferation is mediated by IGF-IR. To characterize the impact of c-myb on the IGF-IGFBP-3 axis in leukemia cells, we overexpressed c-myb using an adenovirus gene transfer system in K562 cells. The overexpression of c-myb induced cell proliferation, compared to control, and c-myb induced cell growth was inhibited by anti-IGF-IR antibodies. c-myb overexpression resulted in a significant increase in the expression of IGF-I, IGF-II, and IGF-IR, and a decrease in IGFBP-3 expression. By contrast, disruption of c-myb function by DN-myb overexpression resulted in significant reduction of IGF-I, IGF-II, IGF-IR, and elevation of IGFBP-3 expression. In addition, exogenous IGFBP-3 inhibited the proliferation of K562 cells, and c-myb induced cell growth was blocked by IGFBP-3 overexpression in a dose-dependent manner. The growth-promoting effects of c-myb were mediated through two major intracellular signaling pathways, Akt and Erk. Activation of Akt and Erk by c-myb was completely blocked by IGF-IR and IGFBP-3 antibodies. These findings suggest that c-myb stimulates cell growth, in part, by regulating expression of the components of IGF-IGFBP axis in K562 cells. In addition, disruption of c-myb function by DN-myb may provide a useful strategy for treatment of leukemia.

c-myb stimulates cell growth by regulation of insulin-like growth factor (IGF) and IGF-binding protein-3 in K562 leukemia cells

International Nuclear Information System (INIS)

Kim, Min-Sun; Kim, Sun-Young; Arunachalam, Sankarganesh; Hwang, Pyoung-Han; Yi, Ho-Keun; Nam, Sang-Yun; Lee, Dae-Yeol

2009-01-01

c-myb plays an important role in the regulation of cell growth and differentiation, and is highly expressed in immature hematopoietic cells. The human chronic myelogenous leukemia cell K562, highly expresses IGF-I, IGF-II, IGF-IR, and IGF-induced cellular proliferation is mediated by IGF-IR. To characterize the impact of c-myb on the IGF-IGFBP-3 axis in leukemia cells, we overexpressed c-myb using an adenovirus gene transfer system in K562 cells. The overexpression of c-myb induced cell proliferation, compared to control, and c-myb induced cell growth was inhibited by anti-IGF-IR antibodies. c-myb overexpression resulted in a significant increase in the expression of IGF-I, IGF-II, and IGF-IR, and a decrease in IGFBP-3 expression. By contrast, disruption of c-myb function by DN-myb overexpression resulted in significant reduction of IGF-I, IGF-II, IGF-IR, and elevation of IGFBP-3 expression. In addition, exogenous IGFBP-3 inhibited the proliferation of K562 cells, and c-myb induced cell growth was blocked by IGFBP-3 overexpression in a dose-dependent manner. The growth-promoting effects of c-myb were mediated through two major intracellular signaling pathways, Akt and Erk. Activation of Akt and Erk by c-myb was completely blocked by IGF-IR and IGFBP-3 antibodies. These findings suggest that c-myb stimulates cell growth, in part, by regulating expression of the components of IGF-IGFBP axis in K562 cells. In addition, disruption of c-myb function by DN-myb may provide a useful strategy for treatment of leukemia.

Farklı Tip Türk Rakılarında Temel Uçucu Bileşiklerin Gaz Kromatografisinde Doğrudan Enjeksiyonla Belirlenmesi

Directory of Open Access Journals (Sweden)

Seda Özkandan

2015-02-01

Full Text Available Bu çalışmada, tek üzüm çeşidinden (Misket, yaş üzümden ve kuru üzüm suması melas alkolü karışımından üretilen farklı tipteki rakıların bileşimleri ve temel uçucu bileşikleri araştırılmıştır. Metanol ve temel uçucu bileşikler (asetaldehit, asetal, metil asetat, etil asetat, 2-bütanol, n-propanol, izobütanol, n-bütanol, aktif amil alkol, izoamil alkol, trans anetol ve estragol AB referans yöntemine göre GC- FID ile belirlenmiştir. Rakıların temel uçucu bileşiklerini, sırasıyla, anason kaynaklı trans anetol ve suma kaynaklı yüksek alkoller, esterler, aldehitler ve uçucu asitler oluşturmuştur. Rakılarda, toplam uçucu madde miktarları uçar asit dahil, 124.99-163.40 g/hL mA arasında, trans anetol miktarları 1038-1380 mg/L arasında, metanol miktarları 43.96-56.91 g/hL mA arasında değişmiştir. En yüksek toplam uçucu madde miktarı Misket üzümünden elde edilen rakıda bulunmuştur. Tek çeşitten ve yaş üzümden üretilen rakıların, karışımdan üretilen rakılara göre daha yüksek miktarlarda toplam uçucu madde ve özellikle yüksek alkoller içerdikleri belirlenmiştir. Yüksek alkol miktarları bakımından tipler arasındaki fark önemli bulunmuştur (P<0.05. Trans anetol miktarı en yüksek yaş üzüm rakısında, en düşük misket rakısında belirlenmiştir. Öte yandan, genel olarak rakıların bileşimlerinin TGK Distile Alkollü İçkiler Tebliği’ne uygun oldukları saptanmıştır.

Jung Işığında Heba Heba In The Light Of Jung

Directory of Open Access Journals (Sweden)

Esra SAZYEK

2013-09-01

Full Text Available Hasan Ali Toptaş, is one of the important authors of Turkishliterature, in his novel called Heba discusses inward looking personalityof Ziya, who lives in the same childish purity during his life consistingof all periods of childhood, military service, marriage and death. Due tothis property, he lives world of dreams and fantasies rather than reeltime. 16 years after he lost his wife and unborn son as casualties inbomb attack, Yazıköy where he breaks away from the crowd of the cityand settle in here, feeds his this side. Thus, the hours passing in thenature, turn into a worship. However, whole peace of Ziya, who hasfound what he seeks in Yazıköy, ends by killing of Kenan. This periodwhen everything has became complicated, is followed by killing of himas being lynched because of the gossips about him.When you reread the novel focusing on a wasted life, of whichtragic events are finished by death in the light of Jungian psychology, itturns into a story of award to reach immortality obtained after thesorrows felt.Experiences of Ziya in Yazıköy resembling Heaven until he isdismissed, realize on the cusp of a magical environment is surroundedby dreams and fantasies. Appearance of unconscious plane by means ofsymbolical contents in dreams indicates that Yazıköy representsunconscious world beyond being a geographic and travelling from thecity to natural follows a way from conscious towards unconscious.Exploring of Ziya moving in unknown darkness of unconsciousthe inner treasure in the deepest, requires passing the each phase fromsurface towards center with a different figure of unconscious named as"archetype" by Jung.In this study, dimensions of period in question will be discussedin terms of Jungian psychology; trails of his thoughts will be examinedin detail by its aspects are reflected on contextual dimension of thenovel. Türk edebiyatının önemli yazarlarından olan Hasan Ali Toptaş, Heba adlı romanında çocukluk, askerlik, evlilik

The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line

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Riki M

2013-02-01

Full Text Available Background: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria (isolated from common carp intestine on K562 and the role of cell concentration on the results of MTT [3-(4,5-Dimethylthiazol-2-yl2,5- Diphenyl tetrazolium Bromide] test.Methods: For this purpose, bacteria were cultured in specific medium (MRS broth at anaerobic condition for 24-48 hour. After incubation period culture medium was centri-fuged, then the cells were washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cell disrupted by Sonication and cell walls were separated from other components by centrifugation. After that, different concentrations of cell walls (500, 1000, 2000 and 4000 µg/ml were prepared in RPMI medium for each bacteria, separately. Then anticancer properties of the cell walls were determined in vitro at 12, 24, 48 and 72 h, also the effect of K562 concentration was assayed with MTT technique.Results: The results showed extracted cell wall from both probiotic statistically (P=0.098 have anti turmeric properties in K562 and their properties will arise in relation with concentration. As well as, we found that the number of cell had not any affect on the result of MTT assay.Conclusion: We conclude that the cytotoxicity property of extracted cell wall is related in the type of bacteria, but this anticancer property would warrant further study on the clinical application of extracted cell wall.

Docosahexaenoic acid and n-6 docosapentaenoic acid supplementation alter rat skeletal muscle fatty acid composition

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Lim Sun-Young

2007-04-01

Full Text Available Abstract Background Docosahexaenoic acid (22:6n-3, DHA and n-6 docosapentaenoic acid (22:5n-6, DPAn-6 are highly unsaturated fatty acids (HUFA, ≥ 20 carbons, ≥ 3 double bonds that differ by a single carbon-carbon double bond at the Δ19 position. Membrane 22:6n-3 may support skeletal muscle function through optimal ion pump activity of sarcoplasmic reticulum and electron transport in the mitochondria. Typically n-3 fatty acid deficient feeding trials utilize linoleic acid (18:2n-6, LA as a comparison group, possibly introducing a lower level of HUFA in addition to n-3 fatty acid deficiency. The use of 22:5n-6 as a dietary control is ideal for determining specific requirements for 22:6n-3 in various physiological processes. The incorporation of dietary 22:5n-6 into rat skeletal muscles has not been demonstrated previously. A one generation, artificial rearing model was utilized to supply 22:6n-3 and/or 22:5n-6 to rats from d2 after birth to adulthood. An n-3 fatty acid deficient, artificial milk with 18:2n-6 was supplemented with 22:6n-3 and/or 22:5n-6 resulting in four artificially reared (AR dietary groups; AR-LA, AR-DHA, AR-DPAn-6, AR-DHA+DPAn-6. A dam reared group (DAM was included as an additional control. Animals were sacrificed at 15 wks and soleus, white gastrocnemius and red gastrocnemius muscles were collected for fatty acid analyses. Results In all muscles of the DAM group, the concentration of 22:5n-6 was significantly lower than 22:6n-3 concentrations. While 22:5n-6 was elevated in the AR-LA group and the AR-DPAn-6 group, 20:4n-6 tended to be higher in the AR-LA muscles and not in the AR-DPAn-6 muscles. The AR-DHA+DPAn-6 had a slight, but non-significant increase in 22:5n-6 content. In the red gastrocnemius of the AR-DPAn-6 group, 22:5n-6 levels (8.1 ± 2.8 wt. % did not reciprocally replace the 22:6n-3 levels observed in AR-DHA reared rats (12.2 ± 2.3 wt. % suggesting a specific preference/requirement for 22:6n-3 in red

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to hyaluronic acid and protection of the skin against dehydration pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Nutrilinks Sarl, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion...

NDA technology for uranium resource evaluation. Progress report July 1-December 31, 1979

International Nuclear Information System (INIS)

Evans, M.L.

1980-08-01

This report describes work performed during the time period from July 1, 1979 to December 31, 1979, on the contract for Nondestructive Nuclear Analysis (NDA) Technology for Uranium Resource Evaluation in Group Q-1. Calculational effort was focused on improving the accuracy with which detector response function maps can be generated for subsequent enfolding with ONETRAN angular flux data. Experimental effort was highlighted by a field test of the prototype photoneutron logging probe at the Grand Junction DOE calibration facility. The probe demonstrated adequate durability in the field and sufficient sensitivity to uranium to function at competitive logging speeds

Mapping of four distinct BCR-related loci to chromosome region 22q11: order of BCR loci relative to chronic myelogenous leukemia and acute lymphoblastic leukemia breakpoints

International Nuclear Information System (INIS)

Croce, C.M.; Huebner, K.; Isobe, M.; Fainstain, E.; Lifshitz, B.; Shtivelman, E.; Canaani, E.

1987-01-01

A probe derived from the 3' region of the BCR gene (breakpoint cluster region gene) detects four distinct loci in the human genome. One of the loci corresponds to the complete BCR gene, whereas the other contain a 3' segment of the gene. After HindIII cleavage of human DNA, these four loci are detected as 23-, 19-, 13-, and 9-kikobase-pair fragments, designated BCR4, BCR3, BCR2, and BCR1, respectively, with BCR1 deriving from the original complete BCR gene. All four BCR loci segregate 100% concordantly with human chromosome 22 in a rodent-human somatic cell hybrid panel and are located at chromosome region 22q11.2 by chromosomal in situ hybridization. The BCR2 and BCR4 loci are amplified in leukemia cell line K562 cells, indicating that they fall within the amplification unit that includes immunoglobulin λ light chain locus (IGL) and ABL locus on the K562 Philadelphia chromosome (Ph 1 ). Similarly, in mouse-human hybrids retaining a Ph 1 chromosome derived from an acute lymphoblastic leukemia-in the absence of the 9q + and 22, only BCR2 and BCR4 loci are retained. Thus, the order of loci on chromosome 22 is centromere → BCR2, BCR4, and IGL → BCR1 → BCR3 → SIS, possibly eliminating BCR2 and BCR4 loci as candidate targets for juxtaposition to the ABL gene in the acute lymphoblastic leukemia Ph 1 chromosome

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for biotin

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for biotin. Biotin is a water-soluble vitamin which serves as a co-factor for several carboxylases that play critical roles in the synthesis...

Nef‛î Divanında Gönül Heart in the Dȋvȃn of Nef‛ȋ’

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Münire Nurefşan KAPAL

2012-09-01

Full Text Available Heart is one of the most common subject which place at Turkish Classical Literature of poetry’s Divan. The concept not only of Classical Literature, but also Folk Literature and oral culture among the topics deal with a lot of culture. İf there are love and lover in the beyit, heart of the lover absolutely take place in the world of the word at the same time. The poet who lived in the first half of the seventeeth century and found fame as a teacher of ode Nef‛î has included the concepts and comparisons in his Dîvân. The word, there are more than in the ghazals. The poet who wrote poetry in general, for the Sultan and statesmen, using exaggerated and a style full of praise; but in his ghazals he writes classic ghazal style and tells as to be in love and enthusiastic words. In his Dîvân, there is no mystical maning of this word. Heart which deal with a variety of interest of the meaning is extased, in love and a mirror showing the beauty lover. In this article, detail definitions of heart concept with various sources will be analyzed together with meanings of words and evaluated by statistical methods. Gönül, klasik edebiyat şairlerinin, divanlarında en çok rastlanan ve işlenen konularından biridir. Kavram sedece klasik edebiyatın değil, halk edebiyatının ve sözlü kültür sahasının da çok işlediği konular arasındadır. Aşk ve âşıklık söz konusu olduğu zaman elbette âşığın gönlü de beyitlerin kelime dünyasında hemen yerini alır. Bu itibarla hangi klasik edebiyat şairinin divanına bakılsa, hemen hemen hepsinde gönül kavramına ait mecazlar ve mazmunlar bulunduğu görülür. XVII. yüzyılın ilk yarısında yaşamış ve bir kaside üstadı olarak şöhret bulmuş olan şair Nef‛î, divanında gönülle ilgili kavram ve benzetmelere yer vermiştir. Kelime, geçiş sıklığı itbariyle daha çok gazellerde yer alır. Kasidelerini genel olarak padişah ve devlet adamlarına ithafen yazan ve m

Docosahexaenoic acid affects arachidonic acid uptake in megakaryocytes

International Nuclear Information System (INIS)

Schick, P.K.; Webster, P.

1987-01-01

Dietary omega 3 fatty acids are thought to prevent atherosclerosis, possibly by modifying platelet (PT) function and arachidonic acid (20:4) metabolism. The study was designed to determine whether omega 3 fatty acids primarily affect 20:4 metabolism in megakaryocytes (MK), bone marrow precursors of PT, rather than in circulating PT. MK and PT were isolated from guinea pigs and incubated with [ 14 C]-20:4 (0.13uM). Docosahexaenoic acid (22:6) is a major omega 3 fatty acid in marine oils. The incubation of MK with 22:6 (0.1, 1.0 uM) resulted in the decrease of incorporation of [ 14 C]-20:4 into total MK phospholipids, 16% and 41% respectively. Alpha-linolenic acid (18:3), a major omega 3 fatty acid present in American diets, had no effect on 20:4 uptake in MK. 22:6 primarily affected the uptake of [ 14 C]-20:4 into phosphatidylethanolamine (PE) and phosphatidylserine (PS) in MK. In MK, 22:6 (0.1, 1.0 uM) caused a decrease of incorporation of [ 14 C]-20:4 into PE, 21% and 55% respectively; a decrease into PS, 16% and 48% respectively; but only a decrease of 4% and 18%, respectively, into phosphatidylcholine; and a decrease of 3% and 21% into phosphatidylinositol 22:6 (3.0 uM) had no effect on the uptake of AA into PT phospholipids. The study shows that 22:6 has a selective effect on AA uptake in MK and that the acylation or transacylation of PE and PS are primarily affected. 22:6 and other marine omega 3 fatty acids appear to primarily affect megakaryocytes which may result in the production of platelets with abnormal content and compartmentalization of AA

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies) , 2013. Scientific Opinion on the safety of “ coriander seed oil ” as a Novel Food ingredient

DEFF Research Database (Denmark)

Tetens, Inge; Poulsen, Morten

were observed when feeding other vegetable oils, although not as severe as that seen for CSO. The dose level of CSO was more than a thousand fold higher than the proposed use level. In a subchronic study using 150, 450 or 1 000 mg/kg bw per day of CSO, a treatment-related effect was observed on blood......Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on “coriander seed oil (CSO)” as a novel food ingredient (NFI) in the context of Regulation (EC) No 258/97. Petroselinic acid (PA......) is the major fatty acid in CSO. Conventional edible oil technologies are used to manufacture the NFI. The NFI is intended to be marketed as a food supplement for healthy adults, at a maximum level of 600 mg per day (i.e. 8.6 mg/kg bw per day for a 70 kg person), which would lead to significantly higher intakes...

Crystallogenesis of bacteriophage P22 tail accessory factor gp26 at acidic and neutral pH

Energy Technology Data Exchange (ETDEWEB)

Cingolani, Gino, E-mail: cingolag@upstate.edu; Andrews, Dewan [Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210 (United States); Casjens, Sherwood [Department of Pathology, Division of Cell Biology and Immunology, University of Utah Medical School, Salt Lake City, UT 84112 (United States); Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210 (United States)

2006-05-01

The crystallogenesis of bacteriophage P22 tail-fiber gp26 is described. To study possible pH-induced conformational changes in gp26 structure, native trimeric gp26 has been crystallized at acidic pH (4.6) and a chimera of gp26 fused to maltose-binding protein (MBP-gp26) has been crystallized at neutral and alkaline pH (7-10). Gp26 is one of three phage P22-encoded tail accessory factors essential for stabilization of viral DNA within the mature capsid. In solution, gp26 exists as an extended triple-stranded coiled-coil protein which shares profound structural similarities with class I viral membrane-fusion protein. In the cryo-EM reconstruction of P22 tail extracted from mature virions, gp26 forms an ∼220 Å extended needle structure emanating from the neck of the tail, which is likely to be brought into contact with the cell’s outer membrane when the viral DNA-injection process is initiated. To shed light on the potential role of gp26 in cell-wall penetration and DNA injection, gp26 has been crystallized at acidic, neutral and alkaline pH. Crystals of native gp26 grown at pH 4.6 diffract X-rays to 2.0 Å resolution and belong to space group P2{sub 1}, with a dimer of trimeric gp26 molecules in the asymmetric unit. To study potential pH-induced conformational changes in the gp26 structure, a chimera of gp26 fused to maltose-binding protein (MBP-gp26) was generated. Hexagonal crystals of MBP-gp26 were obtained at neutral and alkaline pH using the high-throughput crystallization robot at the Hauptman–Woodward Medical Research Institute, Buffalo, NY, USA. These crystals diffract X-rays to beyond 2.0 Å resolution. Structural analysis of gp26 crystallized at acidic, neutral and alkaline pH is in progress.

Uncertainty analysis of NDA waste measurements using computer simulations

International Nuclear Information System (INIS)

Blackwood, L.G.; Harker, Y.D.; Yoon, W.Y.; Meachum, T.R.

2000-01-01

Uncertainty assessments for nondestructive radioassay (NDA) systems for nuclear waste are complicated by factors extraneous to the measurement systems themselves. Most notably, characteristics of the waste matrix (e.g., homogeneity) and radioactive source material (e.g., particle size distribution) can have great effects on measured mass values. Under these circumstances, characterizing the waste population is as important as understanding the measurement system in obtaining realistic uncertainty values. When extraneous waste characteristics affect measurement results, the uncertainty results are waste-type specific. The goal becomes to assess the expected bias and precision for the measurement of a randomly selected item from the waste population of interest. Standard propagation-of-errors methods for uncertainty analysis can be very difficult to implement in the presence of significant extraneous effects on the measurement system. An alternative approach that naturally includes the extraneous effects is as follows: (1) Draw a random sample of items from the population of interest; (2) Measure the items using the NDA system of interest; (3) Establish the true quantity being measured using a gold standard technique; and (4) Estimate bias by deriving a statistical regression model comparing the measurements on the system of interest to the gold standard values; similar regression techniques for modeling the standard deviation of the difference values gives the estimated precision. Actual implementation of this method is often impractical. For example, a true gold standard confirmation measurement may not exist. A more tractable implementation is obtained by developing numerical models for both the waste material and the measurement system. A random sample of simulated waste containers generated by the waste population model serves as input to the measurement system model. This approach has been developed and successfully applied to assessing the quantity of

Nepheliosyne B, a New Polyacetylenic Acid from the New Caledonian Marine Sponge Niphates sp.

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Patrick Auberger

2013-06-01

Full Text Available A new C47 polyoxygenated acetylenic acid, nepheliosyne B (2, along with the previously described nepheliosyne A (1, have been isolated from the New Caledonian marine sponge Niphates sp. Their structures have been elucidated on the basis of extensive spectroscopic analyses. These metabolites exhibited a moderate cytotoxicity against K562, U266, SKM1, and Kasumi cancer cell lines.

Overcoming imatinib resistance using Src inhibitor CGP76030, Abl inhibitor nilotinib and Abl/Lyn inhibitor INNO-406 in newly established K562 variants with BCR-ABL gene amplification.

Science.gov (United States)

Morinaga, Koji; Yamauchi, Takahiro; Kimura, Shinya; Maekawa, Taira; Ueda, Takanori

2008-06-01

Because imatinib (IM) resistance in chronic myeloid leukemia is primarily caused by the re-establishment of Abl kinase, new inhibitors may be efficacious. We evaluated 3 new agents against 2 new K562 variants, IM-R1 and IM-R2 cells, which were developed having 7- and 27-fold greater IM resistance, respectively, than the parental K562 cells. Both variants possessed BCR-ABL gene amplification along with elevated levels of its transcript and protein. Greater BCR-ABL gene amplification was observed in IM-R2 cells than in IM-R1 cells, which was consistent with the higher mRNA and protein levels of Bcr-Abl, and ultimately correlated with the greater IM resistance in IM-R2 cells. No mutation in the Abl kinase domain was detected in either variant. Despite the absence of Lyn overexpression, the Src kinase inhibitor CGP76030 showed positive cooperability with IM in inhibiting cell growth of not only K562 cells but also these 2 variants. This might be because of the augmented inhibition of Erk1/2 phosphorylation. The new Abl kinase inhibitor nilotinib was 10-fold more potent than IM in inhibiting the growth of K562 cells. Nilotinib inhibited the growth of IM-R1 and IM-R2 cells as potently as K562 cells. The combination of nilotinib with CGP76030 showed little additivity, because the potency of nilotinib masked the efficacy of CGP76030. The new dual Abl/Lyn inhibitor INNO-406 (formerly NS-187) was slightly more potent than nilotinib in inhibiting the growth of all 3 cell lines. Because BCR-ABL gene amplification occurs in blast crisis, these inhibitors might overcome IM resistance in such patients' leukemia. (c) 2008 Wiley-Liss, Inc.

Measurement of Nitrogen Hyperfine Structure on the 53 CM (562 MHz) Butyronitrile Line

Science.gov (United States)

Dewberry, Christopher T.; Grubbs, Garry S. Grubbs, II; Raphelt, Andrew; Cooke, Stephen A.

2009-06-01

Recent improvements to our cavity-based Fourier transform radiofrequency spectrometer will be presented. Amongst other improvements use of Miteq amp, model AMF-6F-00100400-10-10P (0.1 GHz to 4 GHz, 65 dB gain minimum, 1 dB noise figure maximum) together with shielding from an improved Faraday cage have significantly helped us in this regard. Electromagnetic fields within our near-spherical cavity have been modeled and results will be presented. We have been able to easily resolve the nitrogen hyperfine structure on the ^aQ_{0,-1} transition 1_{1,0} ← 1_{1,1} located at 562 MHz. This result will be discussed.

Passive Corrosion Behavior of Alloy 22

International Nuclear Information System (INIS)

R.B. Rebak; J.H. Payer

2006-01-01

Alloy 22 (NO6022) was designed to stand the most aggressive industrial applications, including both reducing and oxidizing acids. Even in the most aggressive environments, if the temperature is lower than 150 F (66 C) Alloy 22 would remain in the passive state having particularly low corrosion rates. In multi-ionic solutions that may simulate the behavior of concentrated ground water, even at near boiling temperatures, the corrosion rate of Alloy 22 is only a few nano-meters per year because the alloy is in the complete passive state. The corrosion rate of passive Alloy 22 decreases as the time increases. Immersion corrosion testing also show that the newer generation of Ni-Cr-Mo alloys may offer a better corrosion resistance than Alloy 22 only in some highly aggressive conditions such as in hot acids

Experience of joint use NDA instruments between Japan and IAEA in inspection fields

International Nuclear Information System (INIS)

Yoshii, Hiroshi; Aoki, Minoru; Shimizu, Toku

1997-01-01

In order to implement more effective and efficient safeguards scheme in Japan, Japan and IAEA established joint use program of NDA instruments which was commenced in 1989 from LEU fuel fabrication facilities. The joint use program was proposed by Science and Technology Agency (STA) in the 10th (1989) Joint Japan and IAEA Committee, and it was agreed. Subsequently, Japan and IAEA established working group of the joint use program, whose prepared necessary joint use procedures. Currently, the joint use program has been expanding to almost facilities in domestic, and has been contributing reduce the facility operator's burden for the for inspection activities. (author)

An efficient process for lactic acid production from wheat straw by a newly isolated Bacillus coagulans strain IPE22.

Science.gov (United States)

Zhang, Yuming; Chen, Xiangrong; Luo, Jianquan; Qi, Benkun; Wan, Yinhua

2014-04-01

A thermophilic lactic acid (LA) producer was isolated and identified as Bacillus coagulans strain IPE22. The strain showed remarkable capability to ferment pentose, hexose and cellobiose, and was also resistant to inhibitors from lignocellulosic hydrolysates. Based on the strain's promising features, an efficient process was developed to produce LA from wheat straw. The process consisted of biomass pretreatment by dilute sulfuric acid and subsequent SSCF (simultaneous saccharification and co-fermentation), while the operations of solid-liquid separation and detoxification were avoided. Using this process, 46.12 g LA could be produced from 100g dry wheat straw with a supplement of 10 g/L corn steep liquid powder at the cellulase loading of 20 FPU (filter paper activity units)/g cellulose. The process by B. coagulans IPE22 provides an economical route to produce LA from lignocellulose. Copyright © 2014 Elsevier Ltd. All rights reserved.

Production of 3-Oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic Acid in the Fungus Aspergillus oryzae: A Step Towards Heterologous Production of Pyrethrins in Fungi.

Science.gov (United States)

Mohamed, Maged E; Pahirulzaman, Khomaizon A K; Lazarus, Colin M

2016-03-01

Pyrethrins are natural insecticides, which accumulate to high concentrations in pyrethrum (Chrysanthemum cinerariaefolium) flowers. Synthetic pyrethroids are more stable, more efficacious and cheaper, but contemporary requirements for safe and environmentally friendly pesticides encourage a return to the use of natural pyrethrins, and this would be favoured by development of an efficient route to their production by microbial fermentation. The biosynthesis of pyrethrins involves ester linkage between an acid moiety (chrysanthemoyl or pyrethroyl, synthesised via the mevalonic acid pathway from glucose), and an alcohol (pyrethrolone). Pyrethrolone is generated from 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid, which originates from α-linolenic acid via the jasmonic acid biosynthetic cascade. The first four genes in this cascade, encoding lipoxygenase 2, allene-oxide synthase, allene-oxide cyclase 2 and 12-oxophytodienoic acid reductase 3, were amplified from an Arabidopsis thaliana cDNA library, cloned in a purpose-built fungal multigene expression vector and expressed in Aspergillus oryzae. HPLC-MS analysis of the transgenic fungus homogenate gave good evidence for the presence of 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid.

Stat5 Exerts Distinct, Vital Functions in the Cytoplasm and Nucleus of Bcr-Abl+ K562 and Jak2(V617F)+ HEL Leukemia Cells

International Nuclear Information System (INIS)

Weber, Axel; Borghouts, Corina; Brendel, Christian; Moriggl, Richard; Delis, Natalia; Brill, Boris; Vafaizadeh, Vida; Groner, Bernd

2015-01-01

Signal transducers and activators of transcription (Stats) play central roles in the conversion of extracellular signals, e.g., cytokines, hormones and growth factors, into tissue and cell type specific gene expression patterns. In normal cells, their signaling potential is strictly limited in extent and duration. The persistent activation of Stat3 or Stat5 is found in many human tumor cells and contributes to their growth and survival. Stat5 activation plays a pivotal role in nearly all hematological malignancies and occurs downstream of oncogenic kinases, e.g., Bcr-Abl in chronic myeloid leukemias (CML) and Jak2(V617F) in other myeloproliferative diseases (MPD). We defined the mechanisms through which Stat5 affects growth and survival of K562 cells, representative of Bcr-Abl positive CML, and HEL cells, representative for Jak2(V617F) positive acute erythroid leukemia. In our experiments we suppressed the protein expression levels of Stat5a and Stat5b through shRNA mediated downregulation and demonstrated the dependence of cell survival on the presence of Stat5. Alternatively, we interfered with the functional capacities of the Stat5 protein through the interaction with a Stat5 specific peptide ligand. This ligand is a Stat5 specific peptide aptamer construct which comprises a 12mer peptide integrated into a modified thioredoxin scaffold, S5-DBD-PA. The peptide sequence specifically recognizes the DNA binding domain (DBD) of Stat5. Complex formation of S5-DBD-PA with Stat5 causes a strong reduction of P-Stat5 in the nuclear fraction of Bcr-Abl-transformed K562 cells and a suppression of Stat5 target genes. Distinct Stat5 mediated survival mechanisms were detected in K562 and Jak2(V617F)-transformed HEL cells. Stat5 is activated in the nuclear and cytosolic compartments of K562 cells and the S5-DBD-PA inhibitor most likely affects the viability of Bcr-Abl + K562 cells through the inhibition of canonical Stat5 induced target gene transcription. In HEL cells, Stat5

Hayvan Islahında Güncel Bir Yaklaşım: CRISPR/Cas9 Genom Modifikasyon Sistemi

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Fatih Bilgi

2016-12-01

Full Text Available Genom modifikasyonları, verimin arttırılmasında ve hastalıklara karşı direncin kazandırılması konusunda önemli avantajlar sağlama potansiyeli içermektedir. Bireyin zaten sahip olduğu bir genin susturulmasını ya da ifade edilmesini sağlayan gen düzenlenmesi yöntemleri (gene editing çevreyi en az etkileyerek genetik yapının geliştirilmesi konusunda önemli olanaklar sağlamaktadır. Son zamanlarda yeni genetik düzenleme yöntemleri geliştirilmiştir. Bunlar ZFN (Zinc Finger Nuclease’ler, TALEN (Transcription Activator-like Effector Nuclease’ler ve CRISPR/Cas nükleaz sistemleridir. CRISPR/Cas sistemi yabancı genetik materyalleri yok etmek için RNA güdümlü nükleazları kullanan bir mikrobiyal immün sistem olup, bu sistemin günümüzde hayvanlarda basit ve etkili bir gen düzenleme mekanizması olarak kullanılabilme potansiyeli değerlendirilmektedir. Bu derlemede CRISPR/Cas9 sistemi ve hayvan ıslahında kullanılabilirliği özetlenmiştir.

Recent activities of the ESARDA working group on NDA

International Nuclear Information System (INIS)

Harry, R.J.S.

1983-01-01

The European Safeguards Research and Development Association, ESARDA, has one of the largest coordinated safeguards and development programs in the world. There are several working groups for specific R and D activities. One of these is the ''ESARDA Working Group on Techniques and Standards for non-Destructive Analysis''. The NDA working group has initiated the international project of the preparation of uranium oxide certified reference materials for the gamma spectrometric determination of the enrichment, which are made in a collaboration with the US NBS and the European Communities' Central Bureau for Nuclear Measurements, CBNM, at Geel. The possibility of a similar type of reference material for Pu isotopic abundance measurements is investigated at CBNM, and the pilot samples may become available for intercomparisons. Safeguards acceptability and users manual have been considered carefully. The working group has undertaken an intercomparison on the determination of plutonium isotopic ratios by gamma spectrometry, using NBS-SRM's-946, -947 and 948. A new exercise on 0,5 gram samples of seven different isotopic compositions samples will be executed under the name PIDIE (Plutonium Isotopic Determination Intercomparison Exercise)

Cürufa Olan Bakır Kayıplarında Farklı Kroze Kullanımının Etkisi

OpenAIRE

RÜŞEN, Aydın

2017-01-01

Bakır işletmelerinde ergitme aşamasında cürufa olan bakır kayıpları sektördeki önemli problemlerdenbiridir. Bu çalışmada, Eti Bakır İşletmelerinden (EBİ) temin edilen flaş fırın cürufu ve flaş fırın matı eşitmiktarlarda alınarak farklı oranlarda kolemanit (2CaO·3B2O3·5H2O) ilavesi (%0, %2, %4 ve %6) ilekarıştırılıp alumina (Al2O3) krozelerde 1250oC’de ve azot atmosferi altında 2 saat boyunca ergitmedeneylerine tabi tutulmuştur. Böylece, alümina kroze (AK) kullanılan deneylerde cürufa olan bak...

Topology and parameter data of thirteen non-natural amino acids for molecular simulations with CHARMM22

Directory of Open Access Journals (Sweden)

Olujide O. Olubiyi

2016-12-01

Full Text Available In this article we provide a data package containing the topology files and parameters compatible with the CHARMM22 force field for thirteen non-natural amino acids. The force field parameters were derived based on quantum mechanical (QM calculations involving geometry optimization and potential energy surface scanning at the HF 6-31G(d and HF 6-311G(d,p levels of theory. The resulting energy data points were fitted to mathematical functions representing each component of the CHARMM22 force field. Further fine-tuning of the parameters utilized molecular mechanics energies, which were iteratively calculated and compared to the corresponding QM values until the latter were satisfactorily reproduced. The final force field data were validated with molecular dynamics simulations in explicit solvent conditions.

Oxidative stress by ascorbate/menadione association kills K562 human chronic myelogenous leukaemia cells and inhibits its tumour growth in nude mice.

Science.gov (United States)

Verrax, Julien; Stockis, Julie; Tison, Aurélie; Taper, Henryk S; Calderon, Pedro Buc

2006-09-14

The effect of oxidative stress induced by the ascorbate/menadione-redox association was examined in K562 cells, a human erythromyeloid leukaemia cell line. Our results show that ascorbate enhances menadione redox cycling, leading to the formation of intracellular reactive oxygen species (as shown by dihydrorhodamine 123 oxidation). The incubation of cells in the presence of both ascorbate/menadione and aminotriazole, a catalase inhibitor, resulted in a strong decrease of cell survival, reinforcing the role of H(2)O(2) as the main oxidizing agent killing K562 cells. This cell death was not caspase-3-dependent. Indeed, neither procaspase-3 and PARP were processed and only a weak cytochrome c release was observed. Moreover, we observed only 23% of cells with depolarized mitochondria. In ascorbate/menadione-treated cells, DNA fragmentation was observed without any sign of chromatin condensation (DAPI and TUNEL tests). The cell demise by ascorbate/menadione is consistent with a necrosis-like cell death confirmed by both cytometric profile of annexin-V/propidium iodide labeled cells and by light microscopy examination. Finally, we showed that a single i.p. administration of the association of ascorbate and menadione is able to inhibit the growth of K562 cells by about 60% (in both tumour size and volume) in an immune-deficient mice model. Taken together, these results reinforced our previous claims about a potential application of the ascorbate/menadione association in cancer therapy.

Synthesis and conformational analysis of hybrid α/β-dipeptides incorporating S-glycosyl-β(2,2)-amino acids.

Science.gov (United States)

García-González, Iván; Mata, Lara; Corzana, Francisco; Jiménez-Osés, Gonzalo; Avenoza, Alberto; Busto, Jesús H; Peregrina, Jesús M

2015-01-12

We synthesized and carried out the conformational analysis of several hybrid dipeptides consisting of an α-amino acid attached to a quaternary glyco-β-amino acid. In particular, we combined a S-glycosylated β(2,2)-amino acid and two different types of α-amino acid, namely, aliphatic (alanine) and aromatic (phenylalanine and tryptophan) in the sequence of hybrid α/β-dipeptides. The key step in the synthesis involved the ring-opening reaction of a chiral cyclic sulfamidate, inserted in the peptidic sequence, with a sulfur-containing nucleophile by using 1-thio-β-D-glucopyranose derivatives. This reaction of glycosylation occurred with inversion of configuration at the quaternary center. The conformational behavior in aqueous solution of the peptide backbone and the glycosidic linkage for all synthesized hybrid glycopeptides was analyzed by using a protocol that combined NMR experiments and molecular dynamics with time-averaged restraints (MD-tar). Interestingly, the presence of the sulfur heteroatom at the quaternary center of the β-amino acid induced θ torsional angles close to 180° (anti). Notably, this value changed to 60° (gauche) when the peptidic sequence displayed aromatic α-amino acids due to the presence of CH-π interactions between the phenyl or indole ring and the methyl groups of the β-amino acid unit. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Determining plutonium mass in spent fuel with non-destructive assay techniques - NGSU research overview and update on 6 NDA techniques

Energy Technology Data Exchange (ETDEWEB)

Tobin, Stephen J [Los Alamos National Laboratory; Conlin, Jeremy L [Los Alamos National Laboratory; Evans, Louise G [Los Alamos National Laboratory; Hu, Jianwei [Los Alamos National Laboratory; Blanc, Pauline C [Los Alamos National Laboratory; Lafleur, Adrienne M [Los Alamos National Laboratory; Menlove, Howard O [Los Alamos National Laboratory; Schear, Melissa A [Los Alamos National Laboratory; Swinhoe, Martyn T [Los Alamos National Laboratory; Croft, Stephen [Los Alamos National Laboratory; Fensin, Michael L [Los Alamos National Laboratory; Freeman, Corey R [Los Alamos National Laboratory; Koehler, William E [Los Alamos National Laboratory; Mozin, V [Los Alamos National Laboratory; Sandoval, N P [Los Alamos National Laboratory; Lee, T H [KAERI; Cambell, L W [PNNL; Cheatham, J R [ORNL; Gesh, C J [PNNL; Hunt, A [IDAHO STATE UNIV; Ludewigt, B A [LBNL; Smith, L E [PNNL; Sterbentz, J [INL

2010-09-15

This poster is one of two complementary posters. The Next Generation Safeguards Initiative (NGSI) of the U.S. DOE has initiated a multi-lab/university collaboration to quantify the plutonium (Pu) mass in, and detect the diversion of pins from, spent nuclear fuel assemblies with non-destructive assay (NDA). This research effort has the goal of quantifying the capability of 14 NDA techniques as well as training a future generation of safeguards practitioners. By November of 2010, we will be 1.5 years into the first phase (2.5 years) of work. This first phase involves primarily Monte Carlo modelling while the second phase (also 2.5 years) will focus on experimental work. The goal of phase one is to quantify the detection capability of the various techniques for the benefit of safeguard technology developers, regulators, and policy makers as well as to determine what integrated techniques merit experimental work, We are considering a wide range of possible technologies since our research horizon is longer term than the focus of most regulator bodies. The capability of all of the NDA techniques will be determined for a library of 64 17 x 17 PWR assemblies [burnups (15, 30, 45, 60 GWd/tU), initial enrichments (2, 3, 4, 5%) and cooling times (1, 5, 20, 80 years)]. The burnup and cooling time were simulated with each fuel pin being comprised of four radial regions. In this paper an overview of the purpose will be given as well as a technical update on the following 6 neutron techniques: {sup 252}Cf Interrogation with Prompt Neutron Detection, Delayed Neutrons, Differential Die-Away, Differential Die-Away Self-Interrogation, Passive Neutron Albedo Reactivity, Self-Integration Neutron Resonance Densitometry. The technical update will quantify the anticipated performance of each technique for the 64 assemblies of the spent fuel library.

Determining plutonium mass in spent fuel with non-destructive assay techniques - NGSI research overview and update on 6 NDA techniques

International Nuclear Information System (INIS)

Tobin, Stephen J.; Conlin, Jeremy L.; Evans, Louise G.; Hu, Kianwei; Blanc, P.C.; Lafleur, Am; Menlove, H.O.; Schear, M.A.; Swinhoe, M.T.; Croft, S.; Fensin, M.L.; Freeman, C.R.; Koehler, W.E.; Mozin, V.; Sandoval, N.P.; Lee, T.H.; Cambell, L.W.; Cheatham, J.R.; Gesh, C.J.; Hunt, A.; Ludewigt, B.A.; Smith, L.E.; Sterbentz, J.

2010-01-01

This poster is one of two complementary posters. The Next Generation Safeguards Initiative (NGSI) of the U.S. DOE has initiated a multi-lab/university collaboration to quantify the plutonium (Pu) mass in, and detect the diversion of pins from, spent nuclear fuel assemblies with non-destructive assay (NDA). This research effort has the goal of quantifying the capability of 14 NDA techniques as well as training a future generation of safeguards practitioners. By November of 2010, we will be 1.5 years into the first phase (2.5 years) of work. This first phase involves primarily Monte Carlo modelling while the second phase (also 2.5 years) will focus on experimental work. The goal of phase one is to quantify the detection capability of the various techniques for the benefit of safeguard technology developers, regulators, and policy makers as well as to determine what integrated techniques merit experimental work, We are considering a wide range of possible technologies since our research horizon is longer term than the focus of most regulator bodies. The capability of all of the NDA techniques will be determined for a library of 64 17 x 17 PWR assemblies (burnups (15, 30, 45, 60 GWd/tU), initial enrichments (2, 3, 4, 5%) and cooling times (1, 5, 20, 80 years)). The burnup and cooling time were simulated with each fuel pin being comprised of four radial regions. In this paper an overview of the purpose will be given as well as a technical update on the following 6 neutron techniques: 252 Cf Interrogation with Prompt Neutron Detection, Delayed Neutrons, Differential Die-Away, Differential Die-Away Self-Interrogation, Passive Neutron Albedo Reactivity, Self-Integration Neutron Resonance Densitometry. The technical update will quantify the anticipated performance of each technique for the 64 assemblies of the spent fuel library.

Travma Sonrası Kesici Diş Kırıkları Yüzde Sabit İz kapsamında Değerlendirilmeli mi

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Mehmet Hakan Özdemir

2014-04-01

Full Text Available Türk Ceza Kanununda tanımlanan yaralanma suçlarının adli tıp açısından değerlendirilmesinde kullanılan kriterlerden biri de yüzde sabit iz kavramıdır. Bu suçlarının adli tıp açısından değerlendirilmesinde kullanılan klavuzda, yüzde sabit iz; yüz sınırları içerisinde oluşan yaralanmanın “iyileştikten sonra gün ışığında veya iyi aydınlatılmış ortamda , insanlar arası sözel diyaloğ  mesafesinden (1-2 metre ilk bakışta belirgin bir şekilde fark edilme” durumu olarak değerlendirilmektedir. Dişler ağız içinde yer almakla birlikte aynı zamanda yüz sınırları içindedir. Dinlenme pozisyonunda anatomik olarak dudaklarımız birbirinden hafif açık durmaktadır. Bu konumda özellikle üst kesici dişlerimiz üst dudaktan 2 mm aşağıda görünür haldedir. Cinsiyet farklılıklarına bağlı olarak genç kadınlarda dinlenme pozisyonunda sadece üst kesici dişler görülürken, genç erkeklerde hafif olmakla birlikte alt kesici dişler de görülmektedir. Yaş ilerledikçe yer çekimi kuvvetine bağlı doku sarkmalarıyla tersi bir durum gözlemlenir. Yaş ilerledikçe kadınlarda üst kesici dişler çok az görülürken daha çok alt kesici dişler görülmekte, erkeklerde ise üst kesici dişler görülmeyip  daha belirgin olarak alt kesici dişler görülmektedir. Eğer üst solunum yollarında bir obstrüksiyon varsa, ağızdan nefes alındığı için dudaklar arası açıklık artmakta  ve kesici dişler daha çok görülmektedir.  İnsanlar günlük yaşamında dudakları tam kapalı kalmadığından konuşma, gülme gibi işlevleri sırasında da özellikle kesici dişler görünür pozisyonda olmakta, bu dişlerdeki kırıklar ve eksiklikler daha görünür hale gelmektedir. Yüzde sabit iz ya da yüzün daimi değişikliği kavramları; insanların iletişim kurma, toplum içinde bulunma-yer alma, kendini iyi hissetme anlamında yüzün özel önemi nedeniyle, Türk Ceza

Macrophage inflammatory protein-3α influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells

International Nuclear Information System (INIS)

Lee, Y.C.; Chiou, T.-J.; Tzeng, W.-F.; Chu, S.T.

2008-01-01

Stromal cell monolayers have been an important means of studying the regulation of hematopoiesis, because they produce cytokines. Cytosine arabinoside, vincristine, daunorubicin, and doxorubicin are common drugs for hematological cancer therapy, and they may have some effects on bone marrow stroma during chemotherapy. The aim of this study was to elucidate interactions between the bone marrow stromal microenvironment and leukemic cells after drug treatment. We tested the hypothesis that human HS-5 stromal cells, pretreated with anticancer drugs, affected the growth of leukemic K562 cells by changing the cytokines in the culture microenvironment. Thereafter, proliferation of K562 cells increased nearly 2.5-fold compared the co-cultivation with drugs-pretreated HS-5 stromal cells and drugs-untreated HS-5 stromal cells. The results indicated that co-cultivation with HS-5 stromal cells pretreated with drugs caused significant K562 cell proliferation. Cytokines in the microenvironment were detected via the RayBio Human Cytokine Antibody Array Membrane. The levels of the cytokines CKβ, IL-12, IL-13, IGFBP-2, MCP-1, MCP-3, MCP-4, MDC, MIP-1β and MIP-1δ were decreased, with a particularly marked decrease in MIP-3α. In co-culture medium, there was a 20-fold decrease in MIP-3α in daunorubicin-pretreated HS-5 cells and at least a 3-fold decrease in Ara-C-pretreated cells. This indicated a significant effect of anticancer drugs on the stromal cell line. Using phosphorylated Erk and pRb proteins as cell proliferation markers, we found that phosphorylation of these markers in K562 cells was inhibited during co-cultivation with drug-pretreated stromal cells in MIP-3α-supplemented medium and restored by MIP-3α antibody supplement. In conclusion, anticancer drug pretreatment suppresses the negative control exerted by HS-5 cells on leukemic cell proliferation, via modulation of cytokines in the microenvironment, especially at the level of MIP-3α

PENGARUH EKSTRAK JAMU TERHADAP AKTIVITAS SEL NATURAL KILLER DALAM MELISIS ALUR SEL LEUKIMIA (K-562 SECARA IN VITRO [The Effects of Commercial “Jamu” Extracts on Natural Killer Cell Activity in Lysing Leukemic Cell Line (K-562 in vitro

Directory of Open Access Journals (Sweden)

Elisa Veronica D.C. 2

2002-04-01

Full Text Available Natural killer (NK cell consitutes white blood cells which specifically functions in lysing tumor and virus invected cells. In this research, a commercial “Jamu” was tested to observe its effect on NK cells activity against leukemic cell lines (K562 in vitro. Jamu was extracted with hot water, diluted and added into cell cultures consisted of a mixture of human peripheric limphocyte cells, as the source of the effector NK cells, and K562 cell line i.e., the target cells which were cell line derived from human leukemia and had been labelled with H3-thymidine. The mixture of the cells were made by culturing the two cells at the ratio of 50:1 and 100 : 1, respectively. The results showed that lysing activity of NK cells in the presence of “Jamu” water extract measured as lysing percentage and lysing index increased only slightly, which were not statiscally significant. It should be considered that the test used in this research represents only a part of the lysing mechanism by NK cells against the target cells. An in vivo test for a period of time will be recessary to elucidate ffurther this NK cell activity.

Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells.

Science.gov (United States)

Yamane, Takuya; Sakamoto, Tatsuji; Nakagaki, Takenori; Nakano, Yoshihisa

2018-03-27

The Japanese fermented beverage, homemade kefir, contains six lactic acid bacteria: Lactococcus. lactis subsp. Lactis , Lactococcus . lactis subsp. Cremoris , Lactococcus. Lactis subsp. Lactis biovar diacetylactis , Lactobacillus plantarum , Leuconostoc meseuteroides subsp. Cremoris and Lactobacillus casei . In this study, we found that a mixture of the six lactic acid bacteria from kefir increased the cytotoxicity of human natural killer KHYG-1 cells to human chronic myelogenous leukemia K562 cells and colorectal tumor HCT116 cells. Furthermore, levels of mRNA expression and secretion of IFN-γ (interferon gamma) increased in KHYG-1 cells that had been treated with the six lactic acid bacteria mixture from kefir. The results suggest that the six lactic acid bacteria mixture from kefir has strong effects on natural immunity and tumor cell cytotoxicity.

Nuclear topography of beta-like globin gene cluster in IL-3-stimulated human leukemic K-562 cells

Czech Academy of Sciences Publication Activity Database

Galiová-Šustáčková, Gabriela; Bártová, Eva; Kozubek, Stanislav

2004-01-01

Roč. 33, č. 1 (2004), s. 4-14 ISSN 1079-9796 R&D Projects: GA ČR GA301/01/0186; GA AV ČR KSK5052113; GA AV ČR IAA5004306; GA ČR GA202/04/0907; GA MŠk ME 565 Institutional research plan: CEZ:AV0Z5004920 Keywords : beta-like globin gene cluster * K-562 cells * nuclear topography Subject RIV: BO - Biophysics Impact factor: 2.549, year: 2004

Turizm Ürünlerinin Pazarlanmasında Fiziksel Kanıt Stratejileri

OpenAIRE

Yıldırgan, Recep; Zengin, Burhanettin

2014-01-01

Hizmet sektöründe sunulan ürünler, çoğunlukla soyut özellik taşıdığından bu ürünlerin pazarlamasında bazı somut verilerden yararlanmak gerekmektedir. Turizm işletmeleri de ürünlerini daha etkin pazarlayabilmek için bünyelerindeki fiziksel şartları, mekan, dizayn, ışık, renk, logo, işaret simge, ve bunun gibi unsurlarla somut hale getirerek hedef kitlelerin beğenisine sunarlar. Turizm pazarlaması karması açısından fiziksel kanıt elemanı olarak adlandırılan bu unsurlar, müşterinin turisti...

MiR-27a Promotes Hemin-Induced Erythroid Differentiation of K562 Cells by Targeting CDC25B

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Dongsheng Wang

2018-03-01

Full Text Available Background/Aims: MicroRNAs (miRNAs play a crucial role in erythropoiesis. MiR-23a∼27a∼24-2 clusters have been proven to take part in erythropoiesis via some proteins. CDC25B (cell division control Cdc2 phosphostase B is also the target of mir-27a; whether it regulates erythropoiesis and its mechanism are unknown. Methods: To evaluate the potential role of miR-27a during erythroid differentiation, we performed miR-27a gain- and loss-of-function experiments on hemin-induced K562 cells. We detected miR-27a expression after hemin stimulation at different time points. At the same time, the γ-globin gene also was measured via real-time PCR. According to the results of the chips, we screened the target protein of miR-27a through a dual-luciferase reporter assay and identified it via Western blot analyses. To evaluate the function of CDC25B, benzidine staining and flow cytometry were employed to detect the cell differentiation and cell cycle. Results: We found that miR-27a promotes hemin-induced erythroid differentiation of human K562 cells by targeting cell division cycle 25 B (CDC25B. Overexpression of miR-27a promotes the differentiation of hemin-induced K562 cells, as demonstrated by γ-globin overexpression. The inhibition of miR-27a expression suppresses erythroid differentiation, thus leading to a reduction in the γ-globin gene. CDC25B was identified as a new target of miR-27a during erythroid differentiation. Overexpression of miR-27a led to decreased CDC25B expression after hemin treatment, and CDC25B was up-regulated when miR-27a expression was inhibited. Moreover, the inhibition of CDC25B affected erythroid differentiation, as assessed by γ-globin expression. Conclusion: This study is the first report of the interaction between miR-27a and CDC25B, and it improves the understanding of miRNA functions during erythroid differentiation.

Synthesis, Structural Characterization and Catalytic Activity of A Cu(II Coordination Polymer Constructed from 1,4-Phenylenediacetic Acid and 2,2’-Bipyridine

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Wang Li-Hua

2017-04-01

Full Text Available In order to study the catalytic activity of Cu(II coordination polymer material, a novel 1D chained Cu(II coordination polymer material, [CuL(bipy(H2O5]n (A1 (H2L = 1,4-phenylenediacetic acid, bipy = 2,2’-bipyridine, has been prepared by the reaction of 1,4-phenylenediacetic acid, 2,2’-bipyridine, Cu(CH3COO2·H2O and NaOH. The composition of A1 was determined by elemental analysis, IR spectra and single crystal X-ray diffraction. The results of characterization show that each Cu(II atom adopts six-coordination and forms a distorted octahedral configuration. The catalytic activity and reusability of A1 catalyst for A3 coupling reaction of benzaldehyde, piperidine, and phenylacetylene have been investigated. And the results show that the Cu(II complex catalyst has good catalytic activity with a maximum yield of 54.3% and stability. Copyright © 2017 BCREC GROUP. All rights reserved Received: 21st October 2016; Revised: 17th November 2016; Accepted: 22nd November 2016 How to Cite: Li-Hua, W., Lei, L., Xin, W. (2017. Synthesis, Structural Characterization and Catalytic Activity of A Cu(II Coordination Polymer Constructed from 1,4-Phenylenediacetic Acid and 2,2’-Bipyridine. Bulletin of Chemical Reaction Engineering & Catalysis, 12 (1: 113-118 (doi:10.9767/bcrec.12.1.735.113-118 Permalink/DOI: http://dx.doi.org/10.9767/bcrec.12.1.735.113-118

Small Molecule TH-39 Potentially Targets Hec1/Nek2 Interaction and Exhibits Antitumor Efficacy in K562 Cells via G0/G1 Cell Cycle Arrest and Apoptosis Induction.

Science.gov (United States)

Zhu, Yongxia; Wei, Wei; Ye, Tinghong; Liu, Zhihao; Liu, Li; Luo, Yong; Zhang, Lidan; Gao, Chao; Wang, Ningyu; Yu, Luoting

2016-01-01

Cancer is still a major public health issue worldwide, and new therapeutics with anti-tumor activity are still urgently needed. The anti-tumor activity of TH-39, which shows potent anti-proliferative activity against K562 cells with an IC50 of 0.78 µM, was investigated using immunoblot, co-immunoprecipitation, the MTT assay, and flow cytometry. Mechanistically, TH-39 may disrupt the interaction between Hec1 and Nek2 in K562 cells. Moreover, TH-39 inhibited cell proliferation in a concentration- and time-dependent manner by influencing the morphology of K562 cells and inducing G0/G1 phase arrest. G0/G1 phase arrest was associated with down-regulation of CDK2-cyclin E complex and CDK4/6-cyclin D complex activities. Furthermore, TH-39 also induced cell apoptosis, which was associated with activation of caspase-3, down-regulation of Bcl-2 expression and up-regulation of Bax. TH-39 could also decrease mitochondrial membrane potential (Δψm) and increase reactive oxygen species (ROS) accumulation in K562 cells. The results indicated that TH-39 might induce apoptosis via the ROS-mitochondrial apoptotic pathway. This study highlights the potential therapeutic efficacy of the anti-cancer compound TH-39 in treatment-resistant chronic myeloid leukemia. © 2016 The Author(s) Published by S. Karger AG, Basel.

European Food Safety Authority; Response to comments on the Scientific Opinion of the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) on the scientific substantiation of a health claim related to beta-palmitate and increased calcium absorption pursuant to Article 14 of Regulation (EC

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, EFSA was asked to review the scientific comments received on the Scientific Opinion of the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) on the scientific substantiation of a health claim related to beta-palmitate and increased...... calcium absorption pursuant to Article 14 of Regulation (EC) No 1924/2006. Comments submitted to EFSA via the European Commission Services originated from the applicant (IDACE). EFSA has reviewed the comments and shared them with the chair of the NDA Panel, Prof. Albert Flynn, and the chair of the NDA...

Photodynamic treatment (ALA-PDT) suppresses the expression of the oncogenic Bcr-Abl kinase and affects the cytoskeleton organization in K562 cells

Czech Academy of Sciences Publication Activity Database

Pluskalová, M.; Pešlová, G.; Grebeňová, D.; Halada, Petr; Hrkal, Z.

2006-01-01

Roč. 83, - (2006), s. 205-212 ISSN 1011-1344 R&D Projects: GA MZd NL7681 Institutional research plan: CEZ:AV0Z50200510 Keywords : k562 * bcr -abl * photodynamic treatment Subject RIV: EE - Microbiology, Virology Impact factor: 1.909, year: 2006

6′-Hydroxy Justicidin B Triggers a Critical Imbalance in Ca2+ Homeostasis and Mitochondrion-Dependent Cell Death in Human Leukemia K562 Cells

Directory of Open Access Journals (Sweden)

Jiaoyang Luo

2018-06-01

Full Text Available Justicia procumbens (J. procumbens is a traditional Chinese herbal medicine which was used for the treatment of fever, pain, and cancer. A compound 6′-hydroxy justicidin B (HJB isolated from J. procumbens exhibits promising biological properties. However, the mechanism of action and the in vivo behavior of HJB remain to be elucidated. In this study, we investigated the mechanism of action of HJB on human leukemia K562 cells and its pharmacokinetic properties in rats. The results demonstrated that HJB significantly inhibited the proliferation of K562 cells and promoted apoptosis. Besides, HJB resulted in decreased mitochondrial membrane potential deltaPSIm, increased the level of the calcium homeostasis regulator protein TRPC6 and cytosolic calcium. The activity of caspase-8, caspase-9 and the expression of p53 were significantly increased after treatment with HJB. Additionally, HJB has rapid absorption rate and relative long elimination t1/2, indicating a longer residence time in vivo. The results indicate that HJB inhibited the proliferation of K562 cells and induced apoptosis by affecting the function of mitochondria and calcium homeostasis to activate the p53 signaling pathway. The pharmacokinetic study of HJB suggested it is absorbed well and has moderate metabolism in vivo. These results present HJB as a potential novel alternative to standard human leukemia therapies.

Effects of the antitumoural dequalinium on NB4 and K562 human leukemia cell lines. Mitochondrial implication in cell death.

Science.gov (United States)

Galeano, Eva; Nieto, Elena; García-Pérez, Ana Isabel; Delgado, M Dolores; Pinilla, Montserrat; Sancho, Pilar

2005-10-01

Dequalinium (DQA) is a delocalized lipophylic cation that selectively targets the mitochondria of carcinoma cells. However, the underlying mechanisms of DQA action are not yet well understood. We have studied the effects of DQA on two different leukemia cell lines: NB4, derived from acute promyelocytic leukemia, and K562, derived from chronic myeloid leukemia. We found that DQA displays differential cytotoxic activity in these cell lines. In NB4 cells, a low DQA concentration (2microM) induces a mixture of apoptosis and necrosis, whereas a high DQA concentration (20microM) induces mainly necrosis. However, K562 cell death was always by necrosis as the cells showed a resistance to apoptosis at all time-periods and DQA concentrations assayed. In both cell lines, the cell death seems to be mediated by alterations of mitochondrial function as evidenced by loss of mitochondrial transmembrane potential, O2*- accumulation and ATP depletion. The current study improves the knowledge on DQA as a novel anticancer agent with a potential application in human acute promyelocytic leukemia chemotherapy.

IM-135-562-00 IDIM instruction manual for the isolated digital input module for SLC

International Nuclear Information System (INIS)

Kieffer, J.

1983-01-01

This unit is designed as a general purpose digital input module. Each input is opto-isolated, and is designed to operate over a wide range of positive input voltages. The unit is nonlatching, each CAMAC Read of the unit presenting the data as seen at the inputs at the time of the Read command. The manual includes the following sections: specifications; front panel, lights and connectors; reference list; functional description; 82S100 logic equations; test and checkout procedures; appendix A, SLAC 82S100 programming data; and appendix B, JXK-FORTH 135-562 program listing

THE ARAUCARIA PROJECT: A STUDY OF THE CLASSICAL CEPHEID IN THE ECLIPSING BINARY SYSTEM OGLE LMC562.05.9009 IN THE LARGE MAGELLANIC CLOUD

Energy Technology Data Exchange (ETDEWEB)

Gieren, Wolfgang; Pilecki, Bogumił; Pietrzyński, Grzegorz; Graczyk, Dariusz; Górski, Marek; Taormina, Mónica; Gallenne, Alexandre, E-mail: wgieren@astro-udec.cl, E-mail: pilecki@astrouw.edu.pl, E-mail: pietrzyn@astrouw.edu.pl [Universidad de Concepción, Departamento de Astronomía, Casilla 160-C, Concepción (Chile); and others

2015-12-10

We present a detailed study of the classical Cepheid in the double-lined, highly eccentric eclipsing binary system OGLE-LMC562.05.9009. The Cepheid is a fundamental mode pulsator with a period of 2.988 days. The orbital period of the system is 1550 days. Using spectroscopic data from three 4–8-m telescopes and photometry spanning 22 years, we were able to derive the dynamical masses and radii of both stars with exquisite accuracy. Both stars in the system are very similar in mass, radius, and color, but the companion is a stable, non-pulsating star. The Cepheid is slightly more massive and bigger (M{sub 1} = 3.70 ± 0.03 M{sub ⊙}, R{sub 1} = 28.6 ± 0.2 R{sub ⊙}) than its companion (M{sub 2} = 3.60 ± 0.03 M{sub ⊙}, R{sub 2} = 26.6 ± 0.2 R{sub ⊙}). Within the observational uncertainties both stars have the same effective temperature of 6030 ± 150 K. Evolutionary tracks place both stars inside the classical Cepheid instability strip, but it is likely that future improved temperature estimates will move the stable giant companion just beyond the red edge of the instability strip. Within current observational and theoretical uncertainties, both stars fit on a 205 Myr isochrone arguing for their common age. From our model, we determine a value of the projection factor of p = 1.37 ± 0.07 for the Cepheid in the OGLE-LMC562.05.9009 system. This is the second Cepheid for which we could measure its p-factor with high precision directly from the analysis of an eclipsing binary system, which represents an important contribution toward a better calibration of Baade-Wesselink methods of distance determination for Cepheids.

Corrective Action Plan for Corrective Action Unit 562: Waste Systems, Nevada National Security Site, Nevada

International Nuclear Information System (INIS)

2011-01-01

This Corrective Action Plan has been prepared for Corrective Action Unit (CAU) 562, Waste Systems, in accordance with the Federal Facility Agreement and Consent Order (1996; as amended March 2010). CAU 562 consists of 13 Corrective Action Sites (CASs) located in Areas 2, 23, and 25 of the Nevada National Security Site. Site characterization activities were performed in 2009 and 2010, and the results are presented in Appendix A of the Corrective Action Decision Document for CAU 562. The scope of work required to implement the recommended closure alternatives is summarized. (1) CAS 02-26-11, Lead Shot, will be clean closed by removing shot. (2) CAS 02-44-02, Paint Spills and French Drain, will be clean closed by removing paint and contaminated soil. As a best management practice (BMP), asbestos tile will be removed. (3) CAS 02-59-01, Septic System, will be clean closed by removing septic tank contents. As a BMP, the septic tank will be removed. (4) CAS 02-60-01, Concrete Drain, contains no contaminants of concern (COCs) above action levels. No further action is required; however, as a BMP, the concrete drain will be removed. (5) CAS 02-60-02, French Drain, was clean closed. Corrective actions were completed during corrective action investigation activities. As a BMP, the drain grates and drain pipe will be removed. (6) CAS 02-60-03, Steam Cleaning Drain, will be clean closed by removing contaminated soil. As a BMP, the steam cleaning sump grate and outfall pipe will be removed. (7) CAS 02-60-04, French Drain, was clean closed. Corrective actions were completed during corrective action investigation activities. (8) CAS 02-60-05, French Drain, will be clean closed by removing contaminated soil. (9) CAS 02-60-06, French Drain, contains no COCs above action levels. No further action is required. (10) CAS 02-60-07, French Drain, requires no further action. The french drain identified in historical documentation was not located during corrective action investigation

Stat5 Exerts Distinct, Vital Functions in the Cytoplasm and Nucleus of Bcr-Abl{sup +} K562 and Jak2(V617F){sup +} HEL Leukemia Cells

Energy Technology Data Exchange (ETDEWEB)

Weber, Axel [Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main 60596 (Germany); Borghouts, Corina [Ganymed Pharmaceuticals AG, Mainz 55131 (Germany); Brendel, Christian [Boston Children’s Hospital, Division of Hematology/Oncology, Boston, MA 02115 (United States); Moriggl, Richard [Ludwig Boltzmann Institute for Cancer Research (LBI-CR), Vienna 1090 (Austria); Delis, Natalia; Brill, Boris; Vafaizadeh, Vida; Groner, Bernd, E-mail: Groner@em.uni-frankfurt.de [Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main 60596 (Germany)

2015-03-19

Signal transducers and activators of transcription (Stats) play central roles in the conversion of extracellular signals, e.g., cytokines, hormones and growth factors, into tissue and cell type specific gene expression patterns. In normal cells, their signaling potential is strictly limited in extent and duration. The persistent activation of Stat3 or Stat5 is found in many human tumor cells and contributes to their growth and survival. Stat5 activation plays a pivotal role in nearly all hematological malignancies and occurs downstream of oncogenic kinases, e.g., Bcr-Abl in chronic myeloid leukemias (CML) and Jak2(V617F) in other myeloproliferative diseases (MPD). We defined the mechanisms through which Stat5 affects growth and survival of K562 cells, representative of Bcr-Abl positive CML, and HEL cells, representative for Jak2(V617F) positive acute erythroid leukemia. In our experiments we suppressed the protein expression levels of Stat5a and Stat5b through shRNA mediated downregulation and demonstrated the dependence of cell survival on the presence of Stat5. Alternatively, we interfered with the functional capacities of the Stat5 protein through the interaction with a Stat5 specific peptide ligand. This ligand is a Stat5 specific peptide aptamer construct which comprises a 12mer peptide integrated into a modified thioredoxin scaffold, S5-DBD-PA. The peptide sequence specifically recognizes the DNA binding domain (DBD) of Stat5. Complex formation of S5-DBD-PA with Stat5 causes a strong reduction of P-Stat5 in the nuclear fraction of Bcr-Abl-transformed K562 cells and a suppression of Stat5 target genes. Distinct Stat5 mediated survival mechanisms were detected in K562 and Jak2(V617F)-transformed HEL cells. Stat5 is activated in the nuclear and cytosolic compartments of K562 cells and the S5-DBD-PA inhibitor most likely affects the viability of Bcr-Abl{sup +} K562 cells through the inhibition of canonical Stat5 induced target gene transcription. In HEL cells

Apoptosis induction in MV4-11 and K562 human leukemic cells by Pereskia sacharosa (Cactaceae) leaf crude extract.

Science.gov (United States)

Asmaa, Mat Jusoh Siti; Al-Jamal, Hamid Ali Nagi; Ang, Cheng Yong; Asan, Jamaruddin Mat; Seeni, Azman; Johan, Muhammad Farid

2014-01-01

Pereskia sacharosa is a genus of cacti widely used in folk medicine for cancer-related treatment. Anti-proliferative effects have been studied in recent years against colon, breast, cervical and lung cancer cell lines, with promising results. We here extended study of anti-proliferative effects to a blood malignancy, leukemia. Two leukemic cell lines, MV4-11 (acute myeloid leukemia) and K562 (chronic myeloid leukemia), were studied. IC50 concentrations were determined and apoptosis and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell-cycle related regulatory proteins was assessed by Western blotting. P sacharosa inhibited growth of MV4-11 and K562 cells in a dose-dependent manner. The mode of cell death was via induction of intrinsic apoptotic pathways and cell cycle arrest. There was profound up-regulation of cytochrome c, caspases, p21 and p53 expression and repression of Akt and Bcl-2 expression in treated cells. These results suggest that P sacharosa induces leukemic cell death via apoptosis induction and changes in cell cycle checkpoint, thus deserves further study for anti-leukemic potential.

Identification of cinnabarinic acid as a novel endogenous aryl hydrocarbon receptor ligand that drives IL-22 production.

Science.gov (United States)

Lowe, Margaret M; Mold, Jeff E; Kanwar, Bittoo; Huang, Yong; Louie, Alexander; Pollastri, Michael P; Wang, Cuihua; Patel, Gautam; Franks, Diana G; Schlezinger, Jennifer; Sherr, David H; Silverstone, Allen E; Hahn, Mark E; McCune, Joseph M

2014-01-01

The aryl hydrocarbon receptor (AHR) binds to environmental toxicants including synthetic halogenated aromatic hydrocarbons and is involved in a diverse array of biological processes. Recently, the AHR was shown to control host immunity by affecting the balance between inflammatory T cells that produce IL-17 (Th17) and IL-22 versus regulatory T cells (Treg) involved in tolerance. While environmental AHR ligands can mediate this effect, endogenous ligands are likely to be more relevant in host immune responses. We investigated downstream metabolites of tryptophan as potential AHR ligands because (1) tryptophan metabolites have been implicated in regulating the balance between Th17 and Treg cells and (2) many of the AHR ligands identified thus far are derivatives of tryptophan. We characterized the ability of tryptophan metabolites to bind and activate the AHR and to increase IL-22 production in human T cells. We report that the tryptophan metabolite, cinnabarinic acid (CA), is an AHR ligand that stimulates the differentiation of human and mouse T cells producing IL-22. We compare the IL-22-stimulating activity of CA to that of other tryptophan metabolites and define stimulation conditions that lead to CA production from immune cells. Our findings link tryptophan metabolism to AHR activation and define a novel endogenous AHR agonist with potentially broad biological functions.

Lactic Acid Bacteria from Kefir Increase Cytotoxicity of Natural Killer Cells to Tumor Cells

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Takuya Yamane

2018-03-01

Full Text Available The Japanese fermented beverage, homemade kefir, contains six lactic acid bacteria: Lactococcus. lactis subsp. Lactis, Lactococcus. lactis subsp. Cremoris, Lactococcus. Lactis subsp. Lactis biovar diacetylactis, Lactobacillus plantarum, Leuconostoc meseuteroides subsp. Cremoris and Lactobacillus casei. In this study, we found that a mixture of the six lactic acid bacteria from kefir increased the cytotoxicity of human natural killer KHYG-1 cells to human chronic myelogenous leukemia K562 cells and colorectal tumor HCT116 cells. Furthermore, levels of mRNA expression and secretion of IFN-γ (interferon gamma increased in KHYG-1 cells that had been treated with the six lactic acid bacteria mixture from kefir. The results suggest that the six lactic acid bacteria mixture from kefir has strong effects on natural immunity and tumor cell cytotoxicity.

Palladium-Catalyzed Asymmetric Allylic Alkylation of 4-Substituted Isoxazolidin-5-ones: Straightforward Access to β2,2 -Amino Acids.

Science.gov (United States)

Nascimento de Oliveira, Marllon; Arseniyadis, Stellios; Cossy, Janine

2018-04-03

We report here an unprecedented and highly enantioselective palladium-catalyzed allylic alkylation applied to 4-substituted isoxazolidin-5-ones. Ultimately, the process provides a straightforward access to β 2,2 -amino acids bearing an all-carbon quaternary stereogenic center in great yields and a high degree of enantioselectivity. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Geçmişten Günümüze Edebiyat Ders Planlarında Ve Kitaplarında Yahya Kemal ve Şiirleri From The Past Till Present Day Yahya Kemal And His Poets In Literature Curriculums And Course Books

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Tevfik SÜTÇÜ

2013-07-01

prepared within the framework of literature curriculums ofTurkish Language and Literature course. Türk Edebiyatının XX. yüzyılda yetiştirdiği önemli şairlerinden biri Yahya Kemal Beyatlı’dır. Yahya Kemal, yaşadığı dönemin siyasi, sosyal ve tarihî hadiselerinin içinde yer almasının yanında, Türk edebiyatı ile ilgili önemli ve dikkat çekici hassasiyetleri olan bir şairimizdir. Bütün bu özellikleri ile birlikte Yahya Kemal, Türk şairleri arasında meydana getirdiği eserleri ile Türk dilinin güzel şiir örneklerini vermiş bir şahsiyettir. O, şiirlerinde ele aldığı farklı temaların yanında, şiirlerini meydana getirirken kullandığı dil, biçim ve şekil özellikleri ile öne çıkmış ve şairin bu özelliği hemen her dönemin edebiyat araştırmacıları ve eleştirmenleri tarafından kabul ve teslim edilmiştir.Öğretim kurumlarımızda edebiyat derslerinde edebiyatın diğer türleri ile beraber şiirle ilgili estetik beğenilerin geliştirilmesi ve edebiyat bilgilerinin öğretilmesi hedeflenir. Ülkemizde 1924’ten itibaren okullarımızda bütün diğer ders programları ile beraber edebiyat programlarını yeniden düzenleme çalışmaları da başlar. Cumhuriyet döneminde edebiyat öğretimindeki yönelimlerden birisi, edebiyat derslerinde okutulacak metinlerin çağdaş sanatçıların eserlerinden seçilmesi gerektiği yönündedir. Bu itibarla öğrencilere okuduklarından zevk alması ve yaşayan dilin örnekleriyle karşılaştırılması yanında, bazı metinlerden hareketle edebiyattaki tür ve şekil bilgisine ait konuların öğretilmesi, kavratılması ve tanıtılması mümkün olacaktır. Bütün bu özelliklere sahip olan Yahya Kemal’in eserlerinin ve metinlerinin, okul ders kitaplarında hemen her zaman yer alması isabetli bir tercih olmuştur ve olacaktır.Bu makalede, hangi yıllardan itibaren ve hangi dönemlerde Yahya Kemal’in hangi eserlerinin yoğun olarak ders kitaplarında yer ald

Batı Karadeniz Bölgesi Düzce Ekolojik Koşulları Altında Bazı Ekmeklik Buğday Çeşitlerinin Yaprak Hastalıklarına Karşı Reaksiyonlarının Belirlenmesi

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Nedim Altın

2017-07-01

Full Text Available Bu çalışma, Batı Karadeniz Bölgesi Düzce ili ekolojik koşulları altında 19 adet ekmeklik buğday çeşidinin doğal enfeksiyon koşullarında yaprak hastalıklarına karşı reaksiyonlarının belirlenmesi amacıyla yürütülmüştür. Deneme tesadüf blokları deneme desenine uygun olarak 4 tekerrürlü olarak kurulmuş ve ekim işlemi 17.11.2015 tarihinde yapılmıştır. Hastalık gözlemleri ile ilgili çalışmalar; Septoria tritici etmeninin neden olduğu septorya yaprak lekesi hastalığı için süt olum dönemi başlangıcında, Puccinia striiformis etmeninin neden olduğu Sarı Pas hastalığı için çiçeklenme dönemi sonunda, Puccinia recondita etmeninin neden olduğu Kahverengi Pas için süt olum dönemi başlangıcında yapılmıştır. Tarla koşullarında doğal bulaşıklığa göre yapılan değerlendirme sonucunda hastalık oranları belirlenmiştir. Belirlenen hastalık şiddetine göre, septorya yaprak lekesi hastalığına karşı en hassas çeşidin % 60 hastalık şiddeti ile “Bereket”, en tolerant çeşidin ise %14 ile “Aslı” olduğu, Sarı Pas hastalığına karşı en hassas çeşidin %45,4 ile “Tekirdağ”, en tolerant çeşidin %0,6 ile “Midas” olduğu ve Kahverengi Pas hastalığına karşı ise en hassas çeşidin %22 ile “Tahirova” en tolerant çeşidin ise %0,2 ile “Midas” olduğu belirlenmiştir. Çalışma sonucunda elde edilen verilere göre septorya yaprak lekesi hastalığına karşı Aldane, Aslı, Konya 2002, Köprü, Masaccio ve Tosunbey, Sarı Pas hastalığına karşı Aslı, Esperia, Kate A1, Karasunya Odeska, Masaccio ve Midas, Kahverengi Pas hastalığına karşı ise Aldane, Aslı, Bereket, Köprü, Masaccio, Midas ve Tekirdağ çeşitlerinin ileride yapılacak ıslah çalışmaları için ümitvar olduğu görülmüştür.

Erysipelas of the leg (cellulitis in sub-Saharan Africa: A multicentric study of 562 cases

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Bayaki Saka

2017-01-01

Full Text Available Introduction: Erysipelas of the leg is a common and serious infection. We carried out this study aiming at describing the epidemiological and clinical characteristics, and assessing the risks factors associated with the local complications of erysipelas of the leg in sub-Saharan Africa. Methods: This was a prospective multicentric study carried out in the dermatology units of Hospitals located in seven sub-Saharan African countries during a period of 12 months. Patients aged 15 and above with a first episode of erysipelas of the leg were recruited. Results: In this study, 562 patients were recruited, having a mean age of 43.7±16.9 years and a sex-ratio (M/F of 0.67. Patients infected on one leg were 562 while those infected with two were 27. Bullous forms of the disease were observed in 95 patients, while purpuric forms were observed in 167 patients. The existence of a point of entry (485 cases, obesity (230, lymph edema (130 and the use of bleaching agents (97 were the mains risk factors. Complications during the course of the infection such as necrotizing fasciitis (34 cases and abscesses (63 cases were observed. They were due to the use of antibiotics and non-steroidal anti-inflammatory treatments, and the use of cataplasm. Conclusion: This study reveals that existence of a point of entry, obesity and lymph edema, and the use of bleaching agents were the mains risk factors influencing the local complications of erysipelas of the leg. Necrotizing fasciitis and abscesses were influenced by the use of antibiotics and non-steroidal anti-inflammatory treatments, and the use of cataplasm.

Çocukluk Çağında Anogenıtal Verru ve Cinsel İstismar

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Dua Sumeyra Demirkıran

2014-04-01

Full Text Available Çocuklarda anogenital bölgede cinsel yolla bulaşan hastalıkların varlığı cinsel istismarın delilidir. Human papilloma virüs (HPV yetişkinlerin %80 ini etkileyen, en sık görülen cinsel yolla geçen hastalık kabul edilmektedir. Bu virüsün oluşturduğu verrülerin çocuklarda görülmesi cinsel istismar kanısı oluşturmaktadır. Çalışmamızda anogonital verrü (AGV ile ilgili literatür derlemesi yaparak AGV'li çocuk olgularına adli tıp yönünden yaklaşım önerilerini sunmayı amaçladık. Yapılan çalışmalara göre HPV'nin cinsel istismar dışında farklı bulaş yollarının olduğu bilimsel olarak saptanmıştır. Bu nedenle AGV'li çocuklara yaklaşımda cinsel istismar ön yargısının kırılması gerektiği kanaati oluşmuştur. Çocukların ve ailelerin "cinsel istismar" psikolojisinden uzak tutulmalıdır. Bu olgulara multidisipliner yaklaşılarak bulaş yolu hakkında kanaat oluştuktan sonra adli olgu açısından değerlendirilmelidir. Anahtar kelimeler: anogenital verrü, bulaş yolları, cinsel istismar

Knockdown of HOXA10 reverses the multidrug resistance of human chronic mylogenous leukemia K562/ADM cells by downregulating P-gp and MRP-1.

Science.gov (United States)

Yi, Ying-Jie; Jia, Xiu-Hong; Wang, Jian-Yong; Li, You-Jie; Wang, Hong; Xie, Shu-Yang

2016-05-01

Multidrug resistance (MDR) of leukemia cells is a major obstacle in chemotherapeutic treatment. The high expression and constitutive activation of P-glycoprotein (P-gp) and multidrug resistance protein-1 (MRP-1) have been reported to play a vital role in enhancing cell resistance to anticancer drugs in many tumors. The present study aimed to investigate the reversal of MDR by silencing homeobox A10 (HOXA10) in adriamycin (ADR)-resistant human chronic myelogenous leukemia (CML) K562/ADM cells by modulating the expression of P-gp and MRP-1. K562/ADM cells were stably transfected with HOXA10-targeted short hairpin RNA (shRNA). The results of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis showed that the mRNA and protein expression of HOXA10 was markedly suppressed following transfection with a shRNA-containing vector. The sensitivity of the K562/ADM cells to ADR was enhanced by the silencing of HOXA10, due to the increased intracellular accumulation of ADR. The accumulation of ADR induced by the silencing of HOXA10 may be due to the downregulation of P-gp and MRP-1. Western blot analysis revealed that downregulating HOXA10 inhibited the protein expression of P-gp and MRP-1. Taken together, these results suggest that knockdown of HOXA10 combats resistance and that HOXA10 is a potential target for resistant human CML.

Lead sulfate nano- and microparticles in the acid plant blow-down generated at the sulfuric acid plant of the El Teniente mine, Chile.

Science.gov (United States)

Barassi, Giancarlo M; Klimsa, Martin; Borrmann, Thomas; Cairns, Mathew J; Kinkel, Joachim; Valenzuela, Fernando

2014-12-01

The acid plant 'blow-down' (also called weak acid) produced at El Teniente mine in Chile was characterized. This liquid waste (tailing) is generated during the cooling and cleaning of the smelter gas prior to the production of sulfuric acid. The weak acid was composed of a liquid and a solid phase (suspended solids). The liquid phase of the sample analyzed in this study mainly contained Cu (562 mg L(-1)), SO4(2-) (32 800 mg L(-1)), Ca (1449 mg L(-1)), Fe (185 mg L(-1)), As (6 mg L(-1)), K (467 mg L(-1)) and Al (113 mg L(-1)). Additionally, the sample had a pH-value and total acidity of 0.45 and 2970 mg L(-1) as CaCO3, respectively. Hence, this waste was classified as extremely acidic and with a high metal content following the Ficklin diagram classification. Elemental analysis using atomic absorption, inductively coupled plasma, X-ray diffraction and electron microscopy showed that the suspended solids were anglesite (PbSO4) nano- and microparticles ranging from 50 nm to 500 nm in diameter.

Geçmişten Günümüze Türk Polis Teşkilatında Rütbeler From Past To Present Police Ranks In Turkish National Police

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Bülent AKKAYA

2013-07-01

Full Text Available Law enforcement (police as the Ottoman State and previousperiods was carried out by soldiers and other officials. Until it is calledinternal security service, military police units in carrying out this task.For this reason, the current sense, the police are not able to drive a veryold history. The use of present-day of the word police was in 1845 withthe establishment of the Turkish National Police.That rank highly with the word police, meaning owned degreesand position. Law enforcement the rank of staff education, successfuloperation, owned by a good record to take the title refers to thehierarchical profession. Policing is a career profession. Police officersmust be in a hierarchical structure. Of command within the policesystem degrees and ranks of police officers has to be a profession. Forthis reason, police ranks are quite important in a police organization.Many scientific studies are still being made about TurkishNational Police with the establishment and the development of it.However, historical developments of ranks in Turkish National Policehave not been revealed in a collective manner. In this study scanningthe police ranks of legislative texts, names, profession and evaluationwere performed. Police ranks like Police chief, the Commissioner camefrom Ottoman bureaucracy to Turkish Republic. These police ranksclasses in each other and show continuity with the texts of thelegislation. After giving information about police ranks which used inTurkish National Police, some useful assessments were made for today. Kolluk görevi Osmanlı Devleti ve daha önceki dönemlerde askerler ve diğer görevliler tarafından yerine getirilmiştir. İç güvenlik hizmetini yürüten birimlere polis adı verilene kadar asker kimseler tarafından bu görev yerine getirilmiştir. Bu nedenle bugünkü anlamda polis teşkilatı tarihini çok eskilere götürmek mümkün değildir. Polis kelimesinin günümüzdeki anlamında kullanılması ise 1845 yılında

Polisiye Roman Temsilcisi Ümit Deniz’in Romanlarında Cinayet Kurgusu The Murder Plot in The Novels of Umit Deniz

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Nurullah ULUTAŞ

2012-09-01

Full Text Available Crime fiction, a genre that arose in modern times, deals with the social depression of the bourgeoisie class, which grew bigger in parallel with urbanization. In this genre, the social depression experienced by the modern individual is handled from a crime-centered point of view. The criminal is presented as an afflicted person who suffers from adaptation problems in society. The fact that murder takes a lot of space in these novels turns the concept of death into something ordinary in the minds of modern individuals. This style is carried into fiction through the richness of the rational analytical thinking in Western novels and attracts a large number of readers. The essential elements being mystery, intrigue, tension, crime and murder, the reader is kept alert and interested to follow the sequence of events. The aim of this study is to examine Umit Deniz’s method of creating a murder plot and in the light of the author’s seven novels to look into the psychological impact of murder on society. The techniques that the author makes use of in his writing process are also examined in order to shed light on the difference between crime fiction in Turkish Literature and crime fiction in the West. The main characters, murder plots, modern and traditional patterns, the use of inverted sentences, the fictional means that the author uses in order to keep the story flowing, are reviewed from a technical and theoritical point of view. The plot and any defects therein are criticized from a text-centered point of view. Modern zamanlara özgü bir tür olan polisiye roman, kentleşme ile gelişmeye başlayan burjuvazinin sosyal bunalımlarını suç merkezli olarak ele alır. Suçlunun topluma uyumsuz kişiler olarak yansıtıldığı bu romanlarda, cinayetlere fazla yer verilmesi, modern insan zihninde ölümün sıradanlaşmasına yol açar. Batı romanında rasyonel analitik düşünme zenginliği ile kurguya taşınan bu tarz, geniş okur

Identification of cinnabarinic acid as a novel endogenous aryl hydrocarbon receptor ligand that drives IL-22 production.

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Margaret M Lowe

Full Text Available The aryl hydrocarbon receptor (AHR binds to environmental toxicants including synthetic halogenated aromatic hydrocarbons and is involved in a diverse array of biological processes. Recently, the AHR was shown to control host immunity by affecting the balance between inflammatory T cells that produce IL-17 (Th17 and IL-22 versus regulatory T cells (Treg involved in tolerance. While environmental AHR ligands can mediate this effect, endogenous ligands are likely to be more relevant in host immune responses. We investigated downstream metabolites of tryptophan as potential AHR ligands because (1 tryptophan metabolites have been implicated in regulating the balance between Th17 and Treg cells and (2 many of the AHR ligands identified thus far are derivatives of tryptophan. We characterized the ability of tryptophan metabolites to bind and activate the AHR and to increase IL-22 production in human T cells. We report that the tryptophan metabolite, cinnabarinic acid (CA, is an AHR ligand that stimulates the differentiation of human and mouse T cells producing IL-22. We compare the IL-22-stimulating activity of CA to that of other tryptophan metabolites and define stimulation conditions that lead to CA production from immune cells. Our findings link tryptophan metabolism to AHR activation and define a novel endogenous AHR agonist with potentially broad biological functions.

Synthesis of the Novel 4,4?- and 6,6?- Dihydroxamic - 2,2?-Bipyridines and Improved Routes to 4,4?- and 6,6?- Substituted 2,2?-Bipyridines and Mono-N-Oxide-2,2?-Bipyridine

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Donnici Claudio Luis

1998-01-01

Full Text Available The preparation of key precursors for many 2,2?-bipyridine derivatives such as 4,4?-dicarboxy- 2,2?-bipyridine (I, 6,6?-dicarboxy-2,2?-bipyridine- acid (II, 4,4?-dinitro-2,2?-bipyridine-N,N-dioxide (III, 6,6?-dicarbothioamide-2,2?-bipyridine (IV and mono-N-oxide-2,2?-bipyridine (VII through more efficient methods is described. The syntheses of the novel ligands 4,4?-dihydroxamic-2,2?-bipyridine (V and 6,6?-dihydroxamic-2,2?-bipyridine (VI are also reported.

Evaluation of 22q11.2 deletion in Cleft Palate patients

Science.gov (United States)

Prabodha, L. B. Lahiru; Dias, Dayanath Kumara; Nanayakkara, B. Ganananda; de Silva, Deepthi C.; Chandrasekharan, N. Vishvanath; Ileyperuma, Isurani

2012-01-01

Background: Cleft palate is the commonest multifactorial epigenetic disorder with a prevalence of 0.43-2.45 per 1000. The objectives of this study were to evaluate the clinical features and identify the 22q11.2 deletion in patients with cleft palate in Sri Lanka. Materials and Methods: Cleft patients attending a Teaching Hospital in Sri Lanka were recruited for this study. The relevant data were obtained from review of case notes, interviews, and examination of patients according to a standard evaluation sheet. Quantitative multiplex polymerase chain reaction (PCR) was performed to identify the 22q11.2 deletion. A gel documentation system (Bio-Doc) was used to quantify the PCR product following electrophoresis on 0.8% agarose gel. Results and Conclusion: There were 162 cleft palate patients of whom 59% were females. A total of 92 cleft palate subjects (56.2%) had other associated clinical features. Dysmorphic features (25.27%) and developmental delays (25.27%) were the commonest medical problems encountered. The cleft was limited to the soft palate in 125 patients, while in 25 patients it involved both the hard and the soft palate. There were seven subjects with bifid uvula and five subjects with submucous cleft palate. None of the patients had 22q11.2 deletion in this study population. A multicentered large population-based study is needed to confirm the results of this study and to develop guidelines on the appropriate use of 22q11.2 deletion testing, which are valid for cleft palate patients in Sri Lanka. PMID:23483617

İnsani Güvenlik Kavramı Bağlamında Çevre Güvenliği

Directory of Open Access Journals (Sweden)

Engin İ. ERDEM

2016-12-01

Full Text Available Güvenlik çalışmaları Soğuk Savaş’ın sona ermesinden sonraki dönemde giderek genişleyen bir günde­me sahip olmuştur. Bu makalede genişleyen güvenlik gündeminde yer alan insani güvenlik kavramı bağlamında çevresel güvenlik konusu incelenecektir. Çalışma öncelikle insani güvenlik kavramının ne zaman ortaya çıktığını ve farklı insanı güvenlik tanımlarından bahsetmektedir. 1994 yılı Birleşmiş Milletler Kalkınma Raporu insani güvenlik kavramının ortaya çıkışı açısından dönüm noktası olurken, tanımlar arasında iki tanesi ağırlık kazanmıştır. Geniş tanımlamada yoksulluk ve kalkınma temel dinamikler olarak alınırken dar insani güvenlik tanımlamasında fiziki şiddetin kullanılması baz olarak alınmıştır. Öte yandan, insani güvenlik etrafındaki tartışmaların önemli ölçüde normatif ve kuramsal boyutlara sahip olduğu görülmektedir. Bu sebeple makale başlıca uluslararası ilişkiler teorilerinin in­sani güvenlik olgusuna nasıl baktıklarına da yer vermektedir. Realist teoriler insani güvenlik kavramı ile ilgilenmezken liberal, inşacı ve eleştirel kuramların insani güvenlik olgusu ile ilgili oldukları görül­mektedir. Çalışmanın daha sonraki bölümü başta küresel ısınma olmak üzere çeşitli çevre sorunlarının insani güvenlik ve devletlerarası ilişkiler açısından nasıl tehlike oluşturduklarını analiz etmektedir. Bu bağlamda önemli bir konu çevresel güvensizlik ya da çevre felaketlerinden kaynaklanan devlet içi ve uluslararası göçlerin devletlerin istikrarını bozabilecek olması ve devletlerarası çatışmalara yol açabilme­sidir. Çevresel güvenlik sorunları Darfur ve Suriye’de olduğu gibi sıcak çatışmaların ortaya çıkmasında önemli bir etkiye sahip olabilmektedirler. Çalışmanın sonuç kısmı, insani güvenlik ve çevresel güvenlik kavramlarının güvenlik çalışmaları alan

Kuş Gölü (Bandırma Makrobentik Omurgasız Faunası Hakkında Bir Ön Araştırma.

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Süleyman Balık

2015-12-01

Full Text Available Kuş Gölü’nün makrobentik omurgasız faunasını belirlemek amacıyla 1987 ve 1988 yılları arasında aylık periyotlar halinde, bir yıl süre ile 5 istasyonda arazi çalışması yapılmıştır. Ekman grab ile alınan çamur örnekleri 500 μm göz açıklığındaki elekten geçirilmiş ve örnekler %4’lük formaldehit solüsyonunda tespit edilmiştir. Araştırma sonucunda, Oligochaeta sınıfından 10, Chironomidae familyasından 5 ve Ceratopogonidae familyasından 1 olmak üzere toplam 16 takson tespit edilmiştir. Kuş gölü’nde metrekarede ortalama 6759 birey tespit edilmiş, grupların oransal dağılımında %81,94 ile Oligochaeta bireylerinin en baskın grubu oluşturduğu, bu grubu Chironomidae (%17,90 ve Ceratopogonidae (%0,16 larvalarının takip ettiği saptanmıştır. Tespit edilen grupların istasyonlara göre oransal dağılımlarında ise, 1. istasyonun en zengin (%28,71, 4. istasyonun en fakir (%12,77 istasyon olduğu gözlenmiştir

Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes.

Science.gov (United States)

Phillips, Kyle; Ludidi, Ndiko

2017-08-18

Increased biosynthesis of abscisic acid (ABA) occurs in plants in response to water deficit, which is mediated by changes in the levels of reactive oxygen species such as H 2 O 2 . Water deficit and ABA induce expression of some RD22-like proteins. This study aimed to evaluate the effect of water deficit and exogenous ABA (50 µM ABA applied every 24 hours for a total of 72 hours) on H 2 O 2 content in Zea mays (maize) and to characterise genes encoding two putative maize RD22-like proteins (designated ZmRD22A and ZmRD22B). The expression profiles of the two putative maize RD22-like genes in response to water deficit and treatment with ABA were examined in leaves. In silico analyses showed that the maize RD22-like proteins share domain organisation with previously characterized RD22-like proteins. Both water deficit and exogenous ABA resulted in increased H 2 O 2 content in leaves but the increase was more pronounced in response to water deficit than to exogenous ABA. Lignin content was not affected by exogenous ABA, whereas it was decreased by water deficit. Expression of both RD22-like genes was up-regulated by drought but the ZmRD22A gene was not influenced by exogenous ABA, whereas ZmRD22B was highly responsive to exogenous ABA.

Expression of bovine non-classical major histocompatibility complex class I proteins in mouse P815 and human K562 cells.

Science.gov (United States)

Parasar, Parveen; Wilhelm, Amanda; Rutigliano, Heloisa M; Thomas, Aaron J; Teng, Lihong; Shi, Bi; Davis, William C; Suarez, Carlos E; New, Daniel D; White, Kenneth L; Davies, Christopher J

2016-08-01

Major histocompatibility complex class I (MHC-I) proteins can be expressed as cell surface or secreted proteins. To investigate whether bovine non-classical MHC-I proteins are expressed as cell surface or secreted proteins, and to assess the reactivity pattern of monoclonal antibodies with non-classical MHC-I isoforms, we expressed the MHC proteins in murine P815 and human K562 (MHC-I deficient) cells. Following antibiotic selection, stably transfected cell lines were stained with H1A or W6/32 antibodies to detect expression of the MHC-I proteins by flow cytometry. Two non-classical proteins (BoLA-NC1*00501 and BoLA-NC3*00101) were expressed on the cell surface in both cell lines. Surprisingly, the BoLA-NC4*00201 protein was expressed on the cell membrane of human K562 but not mouse P815 cells. Two non-classical proteins (BoLA-NC1*00401, which lacks a transmembrane domain, and BoLA-NC2*00102) did not exhibit cell surface expression. Nevertheless, Western blot analyses demonstrated expression of the MHC-I heavy chain in all transfected cell lines. Ammonium-sulfate precipitation of proteins from culture supernatants showed that BoLA-NC1*00401 was secreted and that all surface expressed proteins where shed from the cell membrane by the transfected cells. Interestingly, the surface expressed MHC-I proteins were present in culture supernatants at a much higher concentration than BoLA-NC1*00401. This comprehensive study shows that bovine non-classical MHC-I proteins BoLA-NC1*00501, BoLA-NC3*00101, and BoLA-NC4*00201 are expressed as surface isoforms with the latter reaching the cell membrane only in K562 cells. Furthermore, it demonstrated that BoLA-NC1*00401 is a secreted isoform and that significant quantities of membrane associated MHC-I proteins can be shed from the cell membrane. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Kozak Yöresi Fıstıkçamı (Pinus pinea L. ormanlarında fıstık verimi ile artım ve bazı meteorolojik olaylar arasındaki ilişkiler

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Dr. Mustafa BATUR

2016-01-01

Full Text Available Bu çalışmada, Kozak yöresindeki fıstıkçamı ormanlarında fıstık veriminin çap artımı ve meteorolojik olaylarla ilişkileri araştırılmıştır.İlişkilerin ortaya konması için 2006-2011 yılları arasındaki yıllık çap artım verileri, meteorolojik veriler ve örnek ağaçlara ait fıstık verimleri kullanılmıştır.Yapılan değerlendirmede; çap artımı ile fıstık verimi arasında önemli bir bulunamamıştır. Ancak, bazı meteorolojik olaylar ile fıstık verimi arasında ilişkilerin olduğu saptanmıştır. Özellikle, kabuklu fıstık verimi ile bir önceki yılın vejetasyon dönemindeki toplam yağış miktarı arasında R2=0,81, iki yıl önceki vejetasyon dönemindeki toplam yağış miktarı arasında R2=0,79 seviyesinde ilişki saptanmıştır. Fıstık veriminin kozalakların olgunlaştığı yıldaki meteorolojik olaylardan daha çok tozlaşma ve kozalakların büyüme dönemlerindeki meteorolojik verilerle ilişkili olduğu tahmin edilmektedir.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) ; Guidance on the scientific requirements for health claims related to bone, joints, skin and oral health

DEFF Research Database (Denmark)

Tetens, Inge

The Panel on Dietetic Products, Nutrition and Allergies (NDA) has been asked by the European Food Safety Authority (EFSA) to draft guidance on the scientific requirements for health claims related to bone, joints, skin, and oral health. This guidance has been drawn from scientific opinions...

Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans.

Directory of Open Access Journals (Sweden)

Eriko Kage-Nakadai

Full Text Available In multicellular organisms, the surface barrier is essential for maintaining the internal environment. In mammals, the barrier is the stratum corneum. Fatty acid transport protein 4 (FATP4 is a key factor involved in forming the stratum corneum barrier. Mice lacking Fatp4 display early neonatal lethality with features such as tight, thick, and shiny skin, and a defective skin barrier. These symptoms are strikingly similar to those of a human skin disease called restrictive dermopathy. FATP4 is a member of the FATP family that possesses acyl-CoA synthetase activity for very long chain fatty acids. How Fatp4 contributes to skin barrier function, however, remains to be elucidated. In the present study, we characterized two Caenorhabditis elegans genes, acs-20 and acs-22, that are homologous to mammalian FATPs. Animals with mutant acs-20 exhibited defects in the cuticle barrier, which normally prevents the penetration of small molecules. acs-20 mutant animals also exhibited abnormalities in the cuticle structure, but not in epidermal cell fate or cell integrity. The acs-22 mutants rarely showed a barrier defect, whereas acs-20;acs-22 double mutants had severely disrupted barrier function. Moreover, the barrier defects of acs-20 and acs-20;acs-22 mutants were rescued by acs-20, acs-22, or human Fatp4 transgenes. We further demonstrated that the incorporation of exogenous very long chain fatty acids into sphingomyelin was reduced in acs-20 and acs-22 mutants. These findings indicate that C. elegans Fatp4 homologue(s have a crucial role in the surface barrier function and this model might be useful for studying the fundamental molecular mechanisms underlying human skin barrier and relevant diseases.

Comparison of different methods for erythroid differentiation in the K562 cell line.

Science.gov (United States)

Shariati, Laleh; Modaress, Mehran; Khanahmad, Hossein; Hejazi, Zahra; Tabatabaiefar, Mohammad Amin; Salehi, Mansoor; Modarressi, Mohammad Hossein

2016-08-01

To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate, RPMI 1640 medium supplemented with 1 mM sodium butyrate, 25 µg cisplatin/ml, 0.1 µg cytosine arabinoside/ml. The highest differentiation (84 %) with minimum toxicity was obtained with cisplatin at 15 µg /ml. Real-time RT-PCR showed that expression of the γ-globin gene was significantly higher in the cells differentiated with cisplatin compared to undifferentiated cells (P < 0.001). Cisplatin is useful in the experimental therapy of ß-globin gene defects and can be considered for examining the basic mechanism of γ-reactivation.

Stereotipik Davranışların Azaltılmasında Kullanılan Yöntemlerin Etkililiği: Betimsel ve Meta Analizi

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Özlem Toper Korkmaz

2010-07-01

Full Text Available Yineleyici, tekrarlanan davranış olarak tanımlanan stereotipi, farklı gelişimsel yetersizlik türlerinde görülmekle beraber, yaygın olarak otistik bozukluk (otizm gösteren bireylerde de gözlenmekte ve otistik bozukluğun ayırıcı tanı ölçütlerinden birini oluşturmaktadır. Geçmişten günümüze çeşitli yöntemlerin stereotipik davranışların azaltılmasında kullanıldığı görülmektedir. Bu çalışmada da stereotipik davranışların azaltılmasında kullanılan yöntemlerin etkililiklerini incelemek amaçlanmıştır. Bu amaçla 1996 ile 2010 yılları arasında tarama yapılarak otistik bozukluk gösteren bireylerde vokal ya da motor stereotipi veya her ikisinin birden azaltılmasının amaçlandığı toplam 18 araştırma betimsel ve meta analizi yapılarak incelenmiştir. Araştırmanın betimsel analiz sonuçları stereotipik davranışların azaltılmasında kullanılan yöntemlerin etkili olduğunu gösterirken, meta analizi sonuçları yöntemlerin “şüpheli” olduğu sonucunu göstermiştir. Stereotypic behavior is defined repetitive behaviors observed various types of developmental disorders, especially in autism and is also a discriminative criterion to diagnose the autism. Various procedures have been used to reduce the stereotypic behaviors in individuals with these behaviors. The purpose of this study is to investigate the procedures’ effectiveness about reducing the stereotypic behaviors. A total of 18 studies were examined published in between 1996 and 2010 about reducing vocal stereotypy, motor stereotypy or both of them. Descriptive and meta-analysis were carried out on these studies. Whereas results of descriptive analysis revealed that the examined procedures are effective to reduce the stereotypic behaviors, results of meta-analysis showed that these procedures are “uncertain”.

Farklı stres koşullarında Rhodotorula glutinis’ten antioksidan üretimi için biyoproses koşullarının geliştirilmesi

OpenAIRE

ÜNLÜ BÜYÜKTOPÇU, Ayşe Ezgi

2012-01-01

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on Dietary Reference Values for carbohydrates and dietary fibre

OpenAIRE

Tetens, Inge

2011-01-01

This Opinion of the EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA) deals with the establishment of Dietary Reference Values for carbohydrates and dietary fibre. Nutritionally, two broad categories of carbohydrates can be differentiated: “glycaemic carbohydrates”, i.e. carbohydrates digested and absorbed in the human small intestine, and „dietary fibre‟, non-digestible carbohydrates passing to the large intestine. In this Opinion, dietary fibre is defined as non-digestible car...

Ortaöğretim Tarih Ders Kitaplarında Yer Alan Barış ve Antlaşmalar Üzerine Bir Değerlendirme

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Özgür Aktaş

2013-05-01

Full Text Available Bu çalışmanın amacı ortaöğretim tarih ders kitaplarında yer alan antlaşma ve barış konularının içeriğinin nasıl düzenlendiğini değerlendirmektir. Araştırmanın yöntemi doküman analizine dayanmaktadır. Bu çalışmada ders kitaplarında anlatılan antlaşmaların anlatılış tarzları tespit edilmiştir. Antlaşmalar iki gruba ayrılarak değerlendirilmiştir. Birincisi doğrudan anlatılan antlaşmalar, ikincisiyse bir parça ya da metin içerisinde anlatılan antlaşmalardır. Ortaöğretim tarih ders kitaplarına bakıldığında antlaşmalar ve barışlar önemli oranda yer tutmaktadır. Tarih ders kitaplarında savaşlar ve antlaşmalar anlatılırken zengin görsellerin kullanıldığı görülmektedir. Bununla beraber antlaşmalar anlatılırken akıl yürütme, tartışma, eleştirel düşünme gibi tekniklerin kullanılmasında eksiklikler olduğu görülmektedir. Türkiye Cumhuriyeti İnkılâp Tarihi ve Atatürkçülük tarih ders kitabında antlaşmalar çoğunlukla doğrudan anlatılmıştır. Çağdaş Türk ve Dünya Tarihi dersinin programına alınması öğrencilerinde bilmeleri gereken savaşları ve antlaşmaları da fazlasıyla arttırmıştır. Bu antlaşmaların daha iyi anlatılmasının en iyi yolunun konunun öğretimini kolaylaştıracak etkinlik kitaplarının geliştirilmesi olduğu düşünülmektedir. Anahtar Kelimeler: Savaş, Antlaşma, Barış, Tarih, Tarih Eğitimi  A Study into Peaces and Treaties in the History Course Books of Secondary EducationAbstract: The purpose of the current study was to investigate how the content of the treaties and peace subjects in the history course books of secondary education was arranged. The method of the research based on document analysis. In this study, the styles of giving the treaties told in the course books were determined. The treaties were classified in two groups and analysed. First one was the treaties directly told and the second one was

Dağıtım Kanallarında Çatışma Nasıl Önlenebilir?/Prevention of Conflict in Distribution Channels

OpenAIRE

Güreş, Nuriye

2014-01-01

Müşteri memnuniyetinin önem kazandığı günümüzde, müşterinin talep ettiği ürün ve hizmetleri doğru yerde, doğru zamanda ve doğru fiyattan sunmak işletmeler açısından büyük önem arz etmektedir. Bu noktada, ürün ve hizmetlerin müşterilere ulaştırılmasında etkin rol oynayan dağıtım kanallarının önemi ortaya çıkmaktadır. Dolayısıyla bu çalışmada, müşterilere istedikleri ürün ve hizmetleri ulaştırmada çok önemli yeri olan dağıtım kanalları üzerinde durularak, dağıtım kanallarında çeşitli sebeplerl...

Conformationally constrained nucleoside phosphonic acids - potent inhibitors of human mitochondrial and cytosolic 5'(3')-nucleotidases

Czech Academy of Sciences Publication Activity Database

Šimák, Ondřej; Pachl, Petr; Fábry, Milan; Buděšínský, Miloš; Jandušík, T.; Hnízda, Aleš; Skleničková, Radka; Petrová, Magdalena; Veverka, Václav; Řezáčová, Pavlína; Brynda, Jiří; Rosenberg, Ivan

2014-01-01

Roč. 12, č. 40 (2014), s. 7971-7982 ISSN 1477-0520 R&D Projects: GA ČR GA203/09/0820; GA ČR GA13-24880S; GA ČR GA13-26526S; GA MŠk(CZ) LK11205; GA AV ČR KAN200520801 Institutional support: RVO:61388963 ; RVO:68378050 Keywords : 5'(3')-nucleotidase * structure * inhibition * cdN * mdN * nucleoside * SAR * phosphonic acid Subject RIV: CC - Organic Chemistry Impact factor: 3.562, year: 2014

Probing the location of displayed cytochrome b562 on amyloid by scanning tunnelling microscopy

Science.gov (United States)

Forman, C. J.; Wang, N.; Yang, Z. Y.; Mowat, C. G.; Jarvis, S.; Durkan, C.; Barker, P. D.

2013-05-01

Amyloid fibres displaying cytochrome b562 were probed using scanning tunnelling microscopy (STM) in vacuo. The cytochromes are electron transfer proteins containing a haem cofactor and could, in principle, mediate electron transfer between the tip and the gold substrate. If the core fibres were insulating and electron transfer within the 3D haem network was detected, then the electron transport properties of the fibre could be controlled by genetic engineering. Three kinds of STM images were obtained. At a low bias (<1.5 V) the fibres appeared as regions of low conductivity with no evidence of cytochrome mediated electron transfer. At a high bias, stable peaks in tunnelling current were observed for all three fibre species containing haem and one species of fibre that did not contain haem. In images of this kind, some of the current peaks were collinear and spaced around 10 nm apart over ranges longer than 100 nm, but background monomers complicate interpretation. Images of the third kind were rare (1 in 150 fibres); in these, fully conducting structures with the approximate dimensions of fibres were observed, suggesting the possibility of an intermittent conduction mechanism, for which a precedent exists in DNA. To test the conductivity, some fibres were immobilized with sputtered gold, and no evidence of conduction between the grains of gold was seen. In control experiments, a variation of monomeric cytochrome b562 was not detected by STM, which was attributed to low adhesion, whereas a monomeric multi-haem protein, GSU1996, was readily imaged. We conclude that the fibre superstructure may be intermittently conducting, that the cytochromes have been seen within the fibres and that they are too far apart for detectable current flow between sites to occur. We predict that GSU1996, being 10 nm long, is more likely to mediate successful electron transfer along the fibre as well as being more readily detectable when displayed from amyloid.

Poly(lactic acid) (PLA) based nanocomposites-a novel way of drug-releasing

International Nuclear Information System (INIS)

Chen Chen; Lv Gang; Pan Chao; Song Min; Wu Chunhui; Guo Dadong; Wang Xuemei; Chen Baoan; Gu Zhongze

2007-01-01

In this communication, poly(lactic acid) nanofibers have been fabricated by electrospinning and then poly(lactic acid) (PLA) based nanocomposites have been prepared by accumulating anticancer drug daunorubicin on PLA nanofibers combined with TiO 2 nanoparticles. Our atomic force microscopy (AFM) and laser-scanning confocal microscope (LSCM) studies demonstrate that the respective drug molecules could be readily self-assembled on the surface of the blends of nano-TiO 2 with PLA polymer nanocomposites, which could further efficiently facilitate the drug permeation and accumulation on the target leukemia K562 cells. Besides, the respective new nanocomposites have good biocompatibility, ease of surface chemistry modification and very high surface area, which may afford the possibility for their promising application in pharmacology and biomedical engineering areas. (communication)

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on Dietary Reference Values for protein

DEFF Research Database (Denmark)

Tetens, Inge

This opinion of the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) deals with the setting of Dietary Reference Values (DRVs) for protein. The Panel concludes that a Population Reference Intake (PRI) can be derived from nitrogen balance studies. Several health outcomes possibly...... for growth and maintenance. For pregnancy, an intake of 1, 9 and 28 g/d in the first, second and third trimesters, respectively, is proposed in addition to the PRI for non-pregnant women. For lactation, a protein intake of 19 g/d during the first six months, and of 13 g/d after six months, is proposed...

Eğirdir orman fidanlığı’nda diken ardıcı (Juniperus oxycedrus fidan yetiştirme sıklığının fidan morfolojisine etkileri

Directory of Open Access Journals (Sweden)

Esra ALIM

2017-07-01

Full Text Available Bu çalışmada, farklı yetiştirme sıklıklarının diken ardıcı (Juniperus oxycedrus L. subsp. oxycedrus’nın (1+0 çıplak köklü fidanlarının morfolojik özellikleri üzerindeki etkilerini ortaya koymak amaçlanmıştır. Araştırma Eğirdir Orman Fidanlığında kurulan deneme alanlarında yürütülmüştür. Denemede; 1.5 cm, 3 cm, 6 cm ve 9 cm mesafe olacak şekilde kontrol dâhil 5 farklı yetiştirme sıklığı uygulanmıştır. Araştırma sonuçlarına göre diken ardıcı fidanlarının morfolojik özellikleri (kök boğazı çapı, yan kök sayısı, gürbüzlük indisi, kalite indisi, fidan, gövde ve kök taze ağırlıkları ile fidan, gövde ve kök kuru ağırlıkları üzerine yetiştirme sıklığının önemli etkilerinin olduğu belirlenmiştir. Ekim yastıklarında yetiştirme sıklığı azaldıkça daha kalın çaplı, gövde taze ve kuru ağırlığı daha fazla ve daha çok yan kök sayısına sahip olan fidanlar elde edilmiştir. Çalışmada, elde edilen fidanlar arasında en yüksek kök boğazı çapı ve yan kök sayısı kontrol dışındaki ekim sıklıklarından elde edilen fidanlarda olduğu tespit edilmiştir. Fidan ağırlıkları bakımından ise en iyi sonucu 3 cm ekim sıklığı verirken, en düşük sonuç kontrol fidanlarında meydana gelmiştir. Eğirdir Orman Fidanlığı koşullarında 3 cm ekim sıklığının uygulanmasının kaliteli diken ardıcı fidan üretimi için yeterli olacağı düşünülmektedir.

Celecoxib sensitizes imatinib-resistant K562 cells to imatinib by inhibiting MRP1-5, ABCA2 and ABCG2 transporters via Wnt and Ras signaling pathways.

Science.gov (United States)

Dharmapuri, Gangappa; Doneti, Ravinder; Philip, Gundala Harold; Kalle, Arunasree M

2015-07-01

Imatinib mesylate, a tyrosine kinase inhibitor, is very effective in the treatment of chronic myeloid leukemia (CML). However, development of resistance to imatinib therapy is also a very common mechanism observed with long-term administration of the drug. Our previous studies have highlighted the role of cyclooxygenase-2 (COX-2) in regulating the expression of multidrug resistant protein-1 (MDR1), P-gp, in imatinib-resistant K562 cells (IR-K562) via PGE2-cAMP-PKC-NF-κB pathway and inhibition of COX-2 by celecoxib, a COX-2 specific inhibitor, inhibits this pathway and reverses the drug resistance. Studies have identified that not only MDR1 but other ATP-binding cassette transport proteins (ABC transporters) are involved in the development of imatinib resistance. Here, we tried to study the role of COX-2 in the regulation of other ABC transporters such as MRP1, MRP2, MRP3, ABCA2 and ABCG2 that have been already implicated in imatinib resistance development. The results of the study clearly indicated that overexpression of COX-2 lead to upregulation of MRP family proteins in IR-K562 cells and celecoxib down-regulated the ABC transporters through Wnt and MEK signaling pathways. The study signifies that celecoxib in combination with the imatinib can be a good alternate treatment strategy for the reversal of imatinib resistance. Copyright © 2015 Elsevier Ltd. All rights reserved.

Gamma ray NDA assay system for total plutonium and isotopics in plutonium product solutions

International Nuclear Information System (INIS)

Cowder, L.R.; Hsue, S.T.; Johnson, S.S.; Parker, J.L.; Russo, P.A.; Sprinkle, J.K.; Asakura, Y.; Fukuda, T.; Kondo, I.

1979-01-01

A LASL-designed gamma-ray NDA instrument for assay of total plutonium and isotopics of product solutions at Tokai-Mura is currently installed and operating. The instrument is, optimally, a densitometer that uses radioisotopic sources for total plutonium measurements at the K absorption edge. The measured transmissions of additional gamma-ray lines from the same radioisotopic sources are used to correct for self-attenuation of passive gamma rays from plutonium. The corrected passive data give the plutonium isotopic content of freshly separated to moderately aged solutions. This off-line instrument is fully automated under computer control, with the exception of sample positioning, and operates routinely in a mode designed for measurement control. A one-half percent precision in total plutonium concentration is achieved with a 15-minute measurement

Syntheses of 4-aminobutanoic acid-2,2-/sup 2/H/sub 2/ and -4,4-/sup 2/H/sub 2/ and progabide-2,2-/sup 2/H/sub 2/ and -4,4-/sup 2/H/sub 2/

Energy Technology Data Exchange (ETDEWEB)

Davis, B.

1987-10-01

4-Aminobutanoic acid-2,2-/sup 2/H/sub 2/ and -4,4-/sup 2/H/sub 2/ were synthesized in high yield with high deuterium incorporation, and then converted into the corresponding deuterium-labelled anti-convulsant drug, progabide, by means of a transimination reaction.

Gıdaların İyonize Radyasyonla Muhafazasında Radyasyonun Mikroorganizmalar Üzerine Etkileri

OpenAIRE

Özbilgin, Seher; Acar, Jale

2015-01-01

Gıdalarda bulunabilen mikroorganizmalar iyonlaştırıcı radyasyonla öldürülmekte veya gelişmeleri durdurulmaktadır. Bu da iyonlaştırıcı radyasyonun direkt veya indirekt etkisiyle gerçekleşmektedir. Mikroorganizmanın cinsi, vejetatif veya spor formda oluşu, ortamın bileşimi ve sıcaklık gibi faktörler ışınlara karşı duyarlılığı etkilemektedir. Mikroorganizmalar arasında bakteriler, küf ve mayalara kıyasla ışınlara daha dirençli olup, sporlu bakteriler ve özellikle C. botulinum’ un ışınlara karşı...

Continuous gradient temperature Raman spectroscopy of the long chain polyunsaturated fatty acids docosapentaenoic (DPA, 22:5n-6) and docosahexaenoic (DHA; 22:6n-3) from -100 to 20° C

Science.gov (United States)

Broadhurst, C. Leigh; Schmidt, Walter F.; Kim, Moon S.; Nguyen, Julie K.; Qin, Jianwei; Chao, Kuanglin; Bauchan, Gary L.; Shelton, Daniel R.

2016-05-01

The structural, cognitive and visual development of the human brain and retina strictly require long-chain polyunsaturated fatty acids (LC-PUFA). Excluding water, the mammalian brain is about 60% lipid. One of the great unanswered questions with respect to biological science in general is the absolute necessity of the LC-PUFA docosahexaenoic acid (DHA; 22:6n-3) in these fast signal processing tissues. A lipid of the same chain length with just one less diene group, docosapentaenoic acid (DPA; 22:5n-6) is fairly abundant in terrestrial food chains yet cannot substitute for DHA. Gradient Temperature Raman spectroscopy (GTRS) applies the temperature gradients utilized in differential scanning calorimetry to Raman spectroscopy, providing a straightforward technique to identify molecular rearrangements that occur near and at phase transitions. Herein we apply GTRS to DPA, and DHA from -100 to 20°C. 20 Mb three-dimensional data arrays with 1°C increments and first/second derivatives allows complete assignment of solid, liquid and transition state vibrational modes, including low intensity/frequency vibrations that cannot be readily analyzed with conventional Raman. DPA and DHA show significant spectral changes with premelting (-33 and -60°C, respectively) and melting (-27 and -44°C, respectively). The CH2-(HC=CH)-CH2 moieties are not identical in the second half of the DHA and DPA structures. The DHA molecule contains major CH2 twisting (1265 cm-1) with no noticeable CH2 bending, consistent with a flat helical structure with small pitch. Further modeling of neuronal membrane phospholipids must take into account this structure for DHA, which would be configured parallel to the hydrophilic head group line.

Superparamagnetic poly(methyl methacrylate) nanoparticles surface modified with folic acid presenting cell uptake mediated by endocytosis

Energy Technology Data Exchange (ETDEWEB)

Feuser, Paulo Emilio [Federal University of Santa Catarina, Department of Chemical Engineering and Food Engineering (Brazil); Jacques, Amanda Virtuoso [Federal University of Santa Catarina, Department of Clinical Analyses (Brazil); Arévalo, Juan Marcelo Carpio; Rocha, Maria Eliane Merlin [Federal University of Paraná, Department of Biochemistry and Molecular Biology (Brazil); Santos-Silva, Maria Claudia dos [Federal University of Santa Catarina, Department of Clinical Analyses (Brazil); Sayer, Claudia; Araújo, Pedro H. Hermes de, E-mail: pedro.h.araujo@ufsc.br [Federal University of Santa Catarina, Department of Chemical Engineering and Food Engineering (Brazil)

2016-04-15

The encapsulation of superparamagnetic nanoparticles (MNPs) in polymeric nanoparticles (NPs) with modified surfaces can improve targeted delivery and induce cell death by hyperthermia. The goals of this study were to synthesize and characterize surface modified superparamagnetic poly(methyl methacrylate) with folic acid (FA) prepared by miniemulsion polymerization (MNPsPMMA-FA) and to evaluate their in vitro cytotoxicity and cellular uptake in non-tumor cells, murine fibroblast (L929) cells and tumor cells that overexpressed folate receptor (FR) β, and chronic myeloid leukemia cells in blast crisis (K562). Lastly, hemolysis assays were performed on human red blood cells. MNPsPMMA-FA presented an average mean diameter of 135 nm and a saturation magnetization (Ms) value of 37 emu/g of iron oxide, as well as superparamagnetic behavior. The MNPsPMMA-FA did not present cytotoxicity in L929 and K562 cells. Cellular uptake assays showed a higher uptake of MNPsPMMA-FA than MNPsPMMA in K562 cells when incubated at 37 °C. On the other hand, MNPsPMMA-FA showed a low uptake when endocytosis mechanisms were blocked at low temperature (4 °C), suggesting that the MNPsPMMA-FA uptake was mediated by endocytosis. High concentrations of MNPsPMMA-FA showed hemocompatibility when incubated for 24 h in human red blood cells. Therefore, our results suggest that these carrier systems can be an excellent alternative in targeted drug delivery via FR.

Superparamagnetic poly(methyl methacrylate) nanoparticles surface modified with folic acid presenting cell uptake mediated by endocytosis

International Nuclear Information System (INIS)

Feuser, Paulo Emilio; Jacques, Amanda Virtuoso; Arévalo, Juan Marcelo Carpio; Rocha, Maria Eliane Merlin; Santos-Silva, Maria Claudia dos; Sayer, Claudia; Araújo, Pedro H. Hermes de

2016-01-01

The encapsulation of superparamagnetic nanoparticles (MNPs) in polymeric nanoparticles (NPs) with modified surfaces can improve targeted delivery and induce cell death by hyperthermia. The goals of this study were to synthesize and characterize surface modified superparamagnetic poly(methyl methacrylate) with folic acid (FA) prepared by miniemulsion polymerization (MNPsPMMA-FA) and to evaluate their in vitro cytotoxicity and cellular uptake in non-tumor cells, murine fibroblast (L929) cells and tumor cells that overexpressed folate receptor (FR) β, and chronic myeloid leukemia cells in blast crisis (K562). Lastly, hemolysis assays were performed on human red blood cells. MNPsPMMA-FA presented an average mean diameter of 135 nm and a saturation magnetization (Ms) value of 37 emu/g of iron oxide, as well as superparamagnetic behavior. The MNPsPMMA-FA did not present cytotoxicity in L929 and K562 cells. Cellular uptake assays showed a higher uptake of MNPsPMMA-FA than MNPsPMMA in K562 cells when incubated at 37 °C. On the other hand, MNPsPMMA-FA showed a low uptake when endocytosis mechanisms were blocked at low temperature (4 °C), suggesting that the MNPsPMMA-FA uptake was mediated by endocytosis. High concentrations of MNPsPMMA-FA showed hemocompatibility when incubated for 24 h in human red blood cells. Therefore, our results suggest that these carrier systems can be an excellent alternative in targeted drug delivery via FR.

Superparamagnetic poly(methyl methacrylate) nanoparticles surface modified with folic acid presenting cell uptake mediated by endocytosis

Science.gov (United States)

Feuser, Paulo Emilio; Jacques, Amanda Virtuoso; Arévalo, Juan Marcelo Carpio; Rocha, Maria Eliane Merlin; dos Santos-Silva, Maria Claudia; Sayer, Claudia; de Araújo, Pedro H. Hermes

2016-04-01

The encapsulation of superparamagnetic nanoparticles (MNPs) in polymeric nanoparticles (NPs) with modified surfaces can improve targeted delivery and induce cell death by hyperthermia. The goals of this study were to synthesize and characterize surface modified superparamagnetic poly(methyl methacrylate) with folic acid (FA) prepared by miniemulsion polymerization (MNPsPMMA-FA) and to evaluate their in vitro cytotoxicity and cellular uptake in non-tumor cells, murine fibroblast (L929) cells and tumor cells that overexpressed folate receptor (FR) β, and chronic myeloid leukemia cells in blast crisis (K562). Lastly, hemolysis assays were performed on human red blood cells. MNPsPMMA-FA presented an average mean diameter of 135 nm and a saturation magnetization (Ms) value of 37 emu/g of iron oxide, as well as superparamagnetic behavior. The MNPsPMMA-FA did not present cytotoxicity in L929 and K562 cells. Cellular uptake assays showed a higher uptake of MNPsPMMA-FA than MNPsPMMA in K562 cells when incubated at 37 °C. On the other hand, MNPsPMMA-FA showed a low uptake when endocytosis mechanisms were blocked at low temperature (4 °C), suggesting that the MNPsPMMA-FA uptake was mediated by endocytosis. High concentrations of MNPsPMMA-FA showed hemocompatibility when incubated for 24 h in human red blood cells. Therefore, our results suggest that these carrier systems can be an excellent alternative in targeted drug delivery via FR.

Karamık Gölü (Afyonkarahisar'deki Cyprinus carpio Linnaeus, 1758 (Sazan'nun Metazoon Parazitleri Üzerinde Anatomik, Morfolojik ve Ekolojik Bir Araştırma.

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H. Levent Kutlu

2015-12-01

Full Text Available 13 Mart 2004 ile 28 Mayıs 2005 tarihleri arasında yapılan bu araştırmada, Karamık Gölü (Afyonkarahisar’nden yakalanan 71 Cyprinus carpio Linnaeus, 1758 (sazan’nun metazoon parazitleri üzerinde incelemeler yapılmıştır. Konak balıkta üç parazit türü tanımlanmıştır: Gyrodactylus elegans, Nordmann, 1832; Dactylogyrus extensus Mueller ve Van Cleave, 1932 (Monogenoidea, Plathelmintes; Bothriocephalus acheilognathi, Yamaguti, 1934 (Cestoidea, Plathelmintes. Bu türlerden G. elegans 16 balığın yüzgeçlerinde (% 22,5; 7,6 parazit/balık ve 2 balığın solungaçlarında (% 2,8; 5,0 parazit/balık, D. extensus 65 balığın solungaçlarında (% 91,5; 22,1 parazit/balık, B. acheilognathi ise 10 balığın bağırsaklarında (% 14,0; 10,8 parazit/balık görülmüştür. Konak balıktaki dominant parazit türü D. extensus’tur. İlgili parazite ait en yüksek enfeksiyon oranına ve parazit sayısına kış aylarında, en düşük değerlere ise ilkbahar ve yaz aylarında rastlanılmıştır. D. extensus ile enfekte olma oranı küçük boylu sazanlarda büyüklere göre daha fazladır. G. elegans, orta boylu sazanların yüzgeçlerinde ilkbaharda, solungaçlarında ise kış aylarında kaydedilmiştir. Küçük ve orta boylu sazanlarda görülen B. acheilognathi enfeksiyonu kış aylarında yüksek, ilkbahar ve yaz aylarında ise düşük bulunmuştur

Çevre Korunmasında Atığın Atıkla Giderilmesi Prensibi / Principle Of Removal With Waste Of Waste In Environmental Protection

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Utkan Özdemir

2012-12-01

Full Text Available Çevresel problemler göz önüne alındığında, katı atıkların bertarafı birçok dünya ülkesinin temel problemlerinden birisidir. Bu doğrultuda farklı kaynaklarda oluşan ve büyük ölçüde çeşitlilik gösteren katı atıkların bir kısmının tekrar kullanımı hedeflenmektedir. Böylece ekonomik fayda sağlanmaya çalışılmaktadır. Katı atıkların yarattığı çevre kirliliğine, su kaynaklarında meydana gelen ekolojik problemlerin de eklenmesi insanlık için daha büyük risklerin habercisidir. Dolayısıyla katı atıkların bertarafında önemli yeri olan tekrar kullanımın, sadece ekonomik faydası değil, “atığın atıkla giderim” esasına katkısı da tartışılmaya başlanmıştır. Tüketim hızının giderek arttığı dünyada, özellikle tarımsal kökenli atıkların, su arıtımında adsorbent olarak kullanılmasıyla yüksek arıtma verimleri sağlandığı gözlemlenmiştir. Bu durumu takip eden çeşitli araştırmalar, muz kabuğu, ayçiçeği sapı, pirinç kabuğu, portakal kabuğu gibi tarımsal kökenli atıkların yanı sıra kül ve arıtma çamuru gibi atıkların da organik ve inorganik bir takım kirleticilerin su ortamından arıtılmasında önemli rol oynadıklarını göstermiştir. Böylece adsorbent maliyeti nedeniyle çoğu zaman işletmeler tarafından uygun görülmeyen ve pilot ölçekli çalışma olarak kalan adsorpsiyon prosesinin kullanılabilirliğinin arttırılması şansı doğmuştur. Adsorpsiyon proseslerinin yaygınlaştırılması ile atıksu arıtımında elde edilebilecek yüksek verimlerin yanı sıra bu proseslerde adsorbent olarak kullanılan atıkların bertarafı da sağlanmış olacaktır. Aynı zamanda bu durum atıkların başka proseslerde de benzer şekilde değerlendirilmelerinin önünü açmaktadır. Bu çalışmada özellikle endüstriyel bazda kullanımlarında ekonomik ve çevresel faydalar sağlayacak atık kökenli adsorbentlerin türleri ve

Synthesis, characterization and anti cancer activity of some fluorinated 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles

Directory of Open Access Journals (Sweden)

Deepak Chowrasia

2017-05-01

Full Text Available A series of fluorinated 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles (2a–2i was synthesized by condensation of various substituted 4-amino-5-phenyl-4H-1,2,4-triazole-3-thiols (1a–1i with penta fluoro benzoic acid in good yields (60–80%. The synthesized compounds were screened for anticancer activity against three cancerous cell lines MCF7 (human breast cancer, SaOS-2 (human osteosarcoma and K562 (human myeloid leukemia. The compounds showed moderate to good antiproliferative potency against the studied cell lines. Among these, compound 2b showed higher antiproliferative activity (IC50 22.1, 19 and 15 μM against MCF7, SaOS-2 and K562, respectively while 2a exhibited least antiproliferative activity (IC50 30.2, 39 and 29.4 μM against MCF7, SaOS-2 and K562 cells, respectively. Therefore, the present study demonstrates that fluorine substituted 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles would be a better prospective in the development of anticancer drugs.

Marmara Bölgesi Islah Zonu’nda (200-600 m kızılçam (Pinus brutia Ten. döl denemeleri: 12. yaş sonuçları

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Yrd.Doç.Dr. Murat ALAN

2016-07-01

Full Text Available Marmara Bölgesi Islah Zonu’nda seçilen 158 plus ağaç birinci seri ve altı adet tohum bahçesinde bulunan 160 adetklon ikinci seri olacak şekilde gruplandırılmış, toplanan açık tozlaşma ürünü tohumlardan üretilen fidanlarla iki seri kızılçam (Pinus brutia Ten. döl denemesi kurulmuştur. Deneme alanlarında 12. arazi yaşına ait boy ve göğüs çapı ölçülmüş, bu değerler kullanılarak genetik parametreler ve Best Linear Unbiased Prediction (BLUP yöntemi ile ailelerin ıslah değerleri tahmin edilmiştir. Birinci seri deneme alanlarının ortak değerlendirilmesinde boy ve çap için bireysel kalıtım derecesi sırasıyla 0,24 ve 0,16; aile ortalamaları kalıtım derecesi ise 0,52 ve 0,55; ikinci seri deneme alanlarında bireysel kalıtım derecesi aynı sırayla 0,29 ve 0,12; aile ortalamaları kalıtım derecesi ise 0,54 ve 0,45 olarak hesaplanmıştır. Boy ve göğüs çapı arasındaki genetik korelasyon birinci seri denemelerde 0,48 ve ikinci seri denemelerde ise 0,50 olarak tahmin edilmiştir. Dördüncü yaştaki boy ile 12. yaştaki boy arasında genetik korelasyon birinci seri denemelerde 0,81 ve ikinci seri denemelerde ise 0,70 bulunmuştur. B tipi genetik korelasyonlar ise birinci ve ikinci seride 0,54 - 1,00 arasında tahmin edilmiş, genotip çevre etkileşimi açısından uygulamayı etkileyecek düzeyde bulunmamıştır. Birinci seri denemelerde plus ağaç seçimleriyle genetik kazanç düşük olurken, İkinci seri denemelerde fenotipik tohum bahçelerinden boy için %4, göğüs çapı için %5 en iyi 30 klonla genetik tohum bahçesi kurulduğunda ise yine aynı sırayla %13 ve %11 genetik kazanç tahmin edilmiştir. Boy ve göğüs çapı için en yüksek ıslah değerine ulaşan 32, 35, 40 ve 188 nolu tohum bahçeleri, ağaçlandırmalarda kullanıldığında daha fazla boy ve çapa ulaşabilen bireyler elde edilebileceği anlaşılmıştır.Anahtar Kelimeler: Kalıtım derecesi, ıslah de

Kruvaziyer turizmine yönelik tur uygulamalarında değer zinciri analizi

OpenAIRE

Öz, Mehmet Doğan

2015-01-01

Bu çalışma, değer zinciri yöntemini detaylı bir şekilde incelemenin yanı sıra, kruvaziyer turizmi tur organizasyonunda değer zincirinde yer alması gereken değişkenleri ortaya çıkarmak amacını taşımaktadır. Çalışma nitel araştırma yöntemi ile gerçekleştirilmiş olup, veri toplama aşamasında mülakat tekniği kullanılmıştır. Çalışmada, birincil kaynak olarak Aydın’ın Kuşadası ilçesinde bulunan kruvaziyer turizmi ile ilgili olan seyahat acentelerinden faydalanılmıştır. Bu şirketlerin Kuşadası liman...

Bazı Çemen (Trigonella foenum-graecum Hatlarında Yağ ve Yağ Asitleri Bileşenlerinin Araştırılması

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Mehmet Küçük

2015-02-01

Full Text Available Bu çalışmada, Ankara Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü’nde geliştirilen 7 çemen hattının ve 1 standart çeşidin yağ oranları ve yağ asidi bileşenleri araştırılmıştır. Araştırma sonuçlarına göre, yağ oranları %4.01 ile %5.89 arasında değişim göstermiştir. Çemen hatlarında en yüksek yağ asidi olarak linoleik asit bulunmuş, bunu sırasıyla linolenik, oleik ve miristik asitler izlemiştir. Ortalama değerler olarak %44.64 linoleik asit, %23.12 linolenik asit, %19.34 oleik ve %10.24 miristik asit değerleri elde edilmiştir.

Phenethyl isothiocyanate inhibits growth of human chronic myeloid leukemia K562 cells via reactive oxygen species generation and caspases.

Science.gov (United States)

Wang, Yating; Wei, Sixi; Wang, Jishi; Fang, Qin; Chai, Qixiang

2014-07-01

Phenethyl isothiocyanate (PEITC), a potential cancer chemopreventive constituent of cruciferous vegetables, including watercress, has been reported to inhibit cancer cell growth by arresting the cell cycle and inducing apoptosis in various human cancer cell models. However, the role of PEITC in the inhibition of human chronic myeloid leukemia (CML) K562 cell growth and its underlying mechanisms have yet to be elucidated. In the present study, PEITC was found to induce cell death through the induction of reactive oxygen species (ROS) stress and oxidative damage. Heme oxygenase‑1 (HO‑1), which participates in the development of numerous tumors and the sensitivity of these tumors to chemotherapeutic drugs, plays a protective role by modulating oxidative injury. Therefore, the present study assessed the inhibitory effect of PEITC on K562 cells and whether HO‑1 facilitated cell apoptosis and ROS generation. PEITC was found to suppress cell growth and cause apoptosis by promoting Fas and Fas ligand expression, increasing ROS generation and by the successive release of cytochrome c as well as the activation of caspase‑9 and caspase‑3. PEITC was also combined with the HO‑1 inhibitor zinc protoporphyrin IX and the inducer hemin to assess whether HO‑1 determines cell survival and ROS generation. The results of the present study suggest that PEITC may be a potential anti‑tumor compound for CML therapy, and that HO‑1 has a critical function in PEITC‑induced apoptosis and ROS generation.

IM-135-648-00 IDIM tester instruction manual for the TESTER for (135 to 562) Isolated Digital Input Module for SLC

International Nuclear Information System (INIS)

Kieffer, J.

1983-01-01

This unit is designed to provide all of the input levels and channels needed to perform complete production and maintenance testing of the SLC Isolated Digital Input Module (SLAC 135 to 562). The manual includes the following sections: specifications; front panel, lights and connectors; reference list; functional description; 82S100 logic equations; test and checkout procedures; appendix A, SLAC 82S100 programming data; and appendix B, JXK-FORTH 135 to 648 program listing

Ziştovi ve Yaş Antlaşması Arasında Tuna Kıyısında Bir Osmanlı Kenti: Silistre (1791–1793 The Danube Coast Between The Treaty Of Ziştovi And Yaş An Ottoman City: Silistra (1791 – 1793

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Hava SELÇUK

2013-07-01

Full Text Available Silistra Otoman Empire was an important port city on the banksthe Danube River. Therefore, especially in the river trade and battelswere important tasks. The Province of Silistra had a strategical importance by economical and military means. İt was also on the route of Ottoman-Russian and Otoman-Austria wars geographically. Silistra which is consulted both İbrail and rationing of İstanbul has an important function (cureal, hardtack, Weapons etc.. This study has been used as a source the book number 50 of Şeryiyye registries((1791-1793. Before and after the book number 50 in these treaties treaties from Silistra political, social, economic, and military issues contains information . Ser’iyye Sicilleri, that's court records, is an important source in revealing the political, judicial and social conditions of Ottoman society. Seri'yye Sicili Numbered 50 of Silistra, at Bulgarian archive “National Biblioteque”, forms the backbone of this paper. The record book in question includes 233 documents covering (apostasy, Austrian Prisoners such the economic and social events as heritage cases, family, appointment decisions of some officials, ship building, ambassy, tax (cereal, duhan, adat-ı ağnam. Bandit, the grain ship with move conversion topics are explored. The imperial orders (ferman and emir which all shed light on the military issues have been recorded in the ser'iye sicilleri. Based on the court records between 1791 and 1793 of Silistra, it will be mentioned about the social life of the town. Silistre Osmanlı Devleti’nin Tuna Nehri kıyısında bulunan önemlibir liman kenti idi. Bu nedenle özellikle nehir ticareti ve donanmasavaşlarında önemli görevler üslenmekte idi. Ekonomik ve askerî açıdanstratejik bir konuma sahip olan Silistre coğrafi açıdan da Osmanlı-Rusve Osmanlı-Avusturya savaşlarının ana güzergâhı üzerindeydi. Bunedenle Silistre gerek İbrail, gerek İstanbul’un iaşe ihtiyacınınkarşılanmasında

Derin Yapı Yüzey Yapı İlişkisi Bağlamında Temel Dil Becerileri Üzerine Bir Analiz Çalışması

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Bilginer ONAN

2015-07-01

Full Text Available Bu çalışmada, derin yapı yüzey yapı ilişkileri bağlamında, ana dili eğitiminde temel dil becerileri olarak kabul edilen dinleme, konuşma, okuma ve yazma kavramları analiz edilmiştir. Birinci bölümde, ilk kez Port Royal Gramer Okulu tarafından ortaya konan ve 1960’larda Noam Chomsky tarafından Üretimci Dönüşümsel Dil Bilgisi kuramıyla tekrar gündeme getirilen derin yapı ve yüzey yapı kavramları hakkında bilgi verilmiştir. Çalışmanın ikinci bölümünde ise derin yapı ve yüzey yapı ilişkileri çerçevesinde dinleme, konuşma, okuma ve yazma becerileri analiz edilmiştir. Çalışmanın amacı, dilin iki temel boyutu olan anlam ve biçim ilişkileri bağlamında, temel dil becerilerinin eğitimi sürecinde belirleyici olan unsurları tespit ederek, sürecin programlanmasına yönelik öneriler geliştirmektir. Çalışma çerçevesinde yapılan analizlerde boğumlama, bürün olguları, beden dili, üretici kelime hazinesi, alıcı kelime hazinesi, akıcılık, metin türü, yazım ve noktalama, bağdaşıklık, tutarlılık, ön bilgi, kelime tanıma, kelime ayırt etme, edinilmiş bilgi, önvarsayım, sezdirim kavramlarının derin yapı yüzey yapı ilişkileri bağlamında temel dil becerilerinin geliştirilmesinde süreci etkileyen unsurlar olduğu tespit edilmiştir. Bu kavramlar arasında boğumlama, bürün olgusu, beden dili, akıcılık, bağdaşıklık, metin türü, kelime tanıma, kelime ayırt etme, yazım ve noktalama, doğrudan yüzey yapıyla ilgilidir. Üretici kelime hazinesi, akıcılık, alıcı kelime hazinesi, tutarlılık, ön bilgi, edinilmiş bilgi, önvarsayım ve sezdirim kavramları ise derin yapıyı ilgilendirmektedir. Akıcılık kavramının bütün dil becerileriyle ilgili olduğu belirlenmiştir.

NCBI nr-aa BLAST: CBRC-TNIG-22-0088 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-TNIG-22-0088 ref|YP_436579.1| Amino acid transporters [Hahella chejuensis KCTC... 2396] gb|ABC32154.1| Amino acid transporters [Hahella chejuensis KCTC 2396] YP_436579.1 0.16 25% ...

Synthesis, structures and properties of Cu(II) and Mn(II) complexes with 1,10-phenanthroline-2-carboxylic acid and 2,2'-bipyridine ligands.

Science.gov (United States)

Sun, Jingya; Xu, Huanzhi

2010-11-15

Four new 2,2'-bipyridine and 1,10-phenanthroline complexes, namely [Mn(phenca)(2)]·(H(2)O)(2) (1), [Cu(4)(phen)(4)(OH-)(4)(H(2)O)(2)](DMF)(4)(ClO(4)-)(4)(H(2)O) (2), [Cu(2)(2,2-bipy)(2)(C(2)O(4)2-)(H(2)O)(2)(NO(3))(2)] (3) and [Cu(2,2-bipy)(2)(ClO(4)-)](ClO(4)-) (4) (2,2'-bpy = 2,2'-bipyridine, Hphenca = 1,10-phenanthroline-2-carboxylic acid) have been synthesized by a hydrothermal reaction. The products were characterized by elemental analysis, infrared spectroscopy and X-ray crystal diffraction. While strong hydrogen bonds play central roles in the formation of the 3D structure, the combined influence of the weak interactions such as π···π interactions is also evident in the structures. A preliminary investigation on the ion exchange properties of the complexes is presented.

NDA techniques for spent fuel verification and radiation monitoring. Report on activities 6a and 6b of Task JNT C799 (SAGOR). Finnish support programme to the IAEA safeguards

International Nuclear Information System (INIS)

Tarvainen, M.; Levai, F.; Valentine, T.E.; Abhold, M.; Moran, B.

1997-08-01

A variety of NDA methods exist for measurement of spent fuel at various stages of the disposition process. Each of the methods has weaknesses and strengths that make them applicable to one or more stages in disposition. Both passive and active methods are, under favorable conditions, capable of providing either a mapping of an assembly to identify missing fuel pins or a measurement of the fissile content and some are capable of providing a mapping of a canister to identify missing assemblies or a measurement of the fissile content. However, a spent fuel measurement system capable of making routine partial defect tests of spent fuel assemblies is missing. The active NDA methods, in particular, the active neutron methods, hold the most promise for providing quantitative measurements on fuel assemblies and canisters. Application of NDA methods to shielded casks may not be practical or even possible due to the extent of radiation attenuation by the shielding materials, and none of these methods are considered to have potential for quantitative measurements once the spent fuel cask has been placed in a repository. The most practical approach to spent fuel verification is to confirm the characteristics of the spent fuel prior to loading in a canister or cask at the conditioning facility. Fissile material tracking systems in addition to containment and surveillance methods have the capability to assure continuity of the verified knowledge of the sample from loading of the canisters to final disposal and closing of the repository. (orig.)

Three sesquiterpene compounds biosynthesised from artemisinic acid using suspension-cultured cells of Averrhoa carambola (Oxalidaceae).

Science.gov (United States)

Yang, Li; Zhu, Jianhua; Song, Liyan; Shi, Xiaojian; Li, Xingyi; Yu, Rongmin

2012-01-01

A new sesquiterpene glycoside, artemisinic acid 3-β-O-β-D-glucopyranoside (3, 31.24%) and other two biotransformation products, 3-β-hydroxyartemisinic acid (2, 36.69%) and 3-β-hydroxyartemisinic acid β-D-glucopyranosyl ester (4, 7.03%), were biosynthesised after artemisinic acid (1) was administered to the cultured cells of Averrhoa carambola. The three biotransformation products were obtained for the first time by using the suspension-cultured cells of A. carambola as a new biocatalyst system, and their structures were identified on the basis of the physico-chemical properties, NMR and mass spectral analyses. The results indicate that the cultured cells of A. carambola have the abilities to hydroxylate and glycosylate sesquiterpene compounds in a regio- and stereoselective manner. Furthermore, the anti-tumour activity of compounds 3 and 4 was evaluated against K562 and HeLa cell lines. Compound 4 showed strong activity against HeLa cell line, with the IC₅₀ value of 0.56 µmol mL⁻¹.

İkinci Dünya Savaşı Sonrasında Sovyet Tehdidi Karşısında Kalan Türkiye'nin Batı İle İşbirliği Yapma Süreci After the Second World War Against the Soviet Threat in Western Turkey to Cooperate With the Remaining Process

Directory of Open Access Journals (Sweden)

Osman YALÇIN

2013-07-01

Full Text Available Second World War began on the ashes of First World War.Unresolved problems were the main reason that caused The SecondWorld War. Turkey did not bow to pressures and started a new period oftime giving a National Combat. However, Second World War causedimportant problems for Turkey too. Import and export rates decreased.As soon as Second World War was over, although both sides laidtheir arms, Turkey had to keep 1.700.000 armed soldiers. Economicalproblems and being threatened by Soviets were quite important problemareas. In this situation, Turkey tried to find different ways to improve itsrelations with Western countries. American aid was received to meet therequirements of Turkish Army. American aid is known as TrumanDoctrine and Marshal Aid. Turkey which was included into this aid wassupported in this extent. As a result of this support, weapon systems were bought very cheap. After a while, substitute parts of those weapon systems created important cost.Turkey, took pain over being active member of international organizations, too. Being a member of United Nations, Turkey sent troops Korean War and attended the war there. Turkish army showed great bravery. After this success and determination, Turkey strengthened its position in international area being a NATO member. As a result of this situation, Turkey both took itself secure position against Soviet threat and has become a country which gives important support to peace since 1950s. Latest examples of this are Bosnian War, Kosovo Expedition and Afghanistan Expedition. İkinci Dünya Savaşı, Birinci Dünya Savaşı’nın külleri üzerinde başlamıştır. Çözümsüz kalan uluslar arası sorunlar bu savaşın en önemli nedeni olmuştur. Türkiye Batılı devletlerin dayatmalarına boyun eğmemiş ve Milli Mücadele vererek yeni bir dönem başlatmıştır. Ne var ki, 2. Dünya Savaşı Türkiye için de önemli sorunlara neden olmuştur. Savaş yıllarında ithalat ve ihracat miktarı d

Zile Elbaşoğlu Camii’nin Sıvalar Altında Kalan Gizemi The Mystery Of The Zile-Elbaşoğlu Mosque Remains Under Plasters

Directory of Open Access Journals (Sweden)

Ali Murat AKTEMUR

2013-09-01

Full Text Available Elbaşoğlu Mosque was built in 1801 by Ahmet Aga, clean-cutstone architecture, square, rectangular with remarkable inscriptionsElbaşoğlu Mosque is one of the local mosque, wood-backed and woodenceilings. Until 2007, the walls and the ceiling, and even the altar,wooden support, wood columns, wooden gallery, galleries, in short, allthe wooden elements of the mosque was covered with oil on sweet-limeplaster. In 2007, during the repair of the roof of a simple chance, withplaster falling due to the flowing water under the pen-and-repair workon the emergence of ornaments and wooden decorations samples, thenstopped cleaning and scraper operations in the administration of theGeneral Directorate of Foundations in the interior of the mosque penrichsamples of work, with examples of wooden decorative plaster underthe mystery was revealed.Cover at the top of the walls combined with Harim calligraphy ona black background with spots, including a concave border of the beltframed verse was written. Verse generation raised on the corners of theceiling moldings created crash of beads, triangle, octagon in the middleof a field, and thus created the split surfaces. Located in the middle ofthe octagon octagon-shaped belly, finished with carving technique,baroque decorated with floral motifs. the four corners of the triangularroof panels are decorated with reliefs excess plastic plant. Pen-workdecoration floral motifs on the walls, gallery, pulpits, pillars, such asthe carved wooden elements fall floral and geometric motifs are carvedin the mosque offers a wealth of eye-catching as almost free space left.Made in 1801, in the early years of the Republic, the region'sunique sweet-lime paint on plaster taken off this rich decoration, woodart and pen-work ornamentation Turkey offers one of the richest. Tokat Zile’de Elbaşoğlu Ahmet Ağa tarafından 1801 yılında inşa ettirilen, düzgün kesme taş mimarisi, kitabeleri ile dikkat çeken, kareye yakın dikd

Nicole Krauss’un Aşkın Tarihi Adlı Romanında Tarihi İzlekler

OpenAIRE

KALAY, Faruk

2014-01-01

Modern edebiyatta önemli Yahudi Amerikan yazarlarından olan Nicole Krauss Yahuditoplumundaki yabancılaşma, yalnızlık ve göç konularına değinir. Başarılı romanı olanAşkın Tarihi II. Dünya Savaşı süresince Holokost’a maruz kalan ve etkilerini hissedenYahudi karakterle ilgilidir. Tarihle bütünleşen bu karakterler gerçek hayattakiinsanlardan ve olaylardan esinlenmiştir. Krauss romanında postmodern teknikleri dekullanır. Örneğin iç içe anlatım tekniği okuyucuya romanın kurgusal mı yoksa gerçek mio...

Applications of Poly(indole-6-carboxylic acid-co-2,2′-bithiophene Films in High-Contrast Electrochromic Devices

Directory of Open Access Journals (Sweden)

Chung-Wen Kuo

2018-03-01

Full Text Available Two homopolymers (poly(indole-6-carboxylic acid (PInc and poly(2,2′-bithiophene (PbT and a copolymer (poly(indole-6-carboxylic acid-co-2,2′-bithiophene (P(Inc-co-bT are electrodeposited on ITO electrode surfaces via electrochemical method. Electrochemical and electrochromic properties of PInc, PbT, and P(Inc-co-bT films were characterized using cyclic voltammetry and in situ UV-Vis spectroscopy. The anodic P(Inc-co-bT film prepared using Inc./bT = 1/1 feed molar ratio shows high optical contrast (30% at 890 nm and coloring efficiency (112 cm2 C−1 at 890 nm. P(Inc-co-bT film revealed light yellow, yellowish green, and bluish grey in the neutral, intermediate, and oxidation states, respectively. Electrochromic devices (ECDs were constructed using PInc, PbT, or P(Inc-co-bT film as anodic layer and PEDOT-PSS as cathodic layer. P(Inc-co-bT/PMMA-PC-ACN-LiClO4/PEDOT-PSS ECD showed high ∆T (31% at 650 nm, and PInc/PMMA-PC-ACN-LiClO4/PEDOT-PSS ECD displayed high coloration efficiency (416.7 cm2 C−1 at 650 nm. The optical memory investigations of PInc/PMMA-PC-ACN-LiClO4/PEDOT-PSS, PbT/PMMA-PC-ACN-LiClO4/PEDOT-PSS, and P(Inc-co-bT/PMMA-PC-ACN-LiClO4/PEDOT-PSS ECDs exhibited that ECDs had adequate optical memory in bleaching and coloring states.

Bioethanol production from paperboard mill sludge using acid-catalyzed bio-derived choline acetate ionic liquid pretreatment followed by fermentation process

International Nuclear Information System (INIS)

Farghaly, Ahmed; Elsamadony, Mohamed; Ookawara, Shinichi; Tawfik, Ahmed

2017-01-01

Highlights: • Total reducing sugar concentration depends on [ChO][OAc] ionic liquid to biomass ratio. • Acid-catalyzed ionic liquid significantly enhance pretreatment process. • Prolonged pretreatment duration degraded sugars into furans compounds. • Maximum net energy of 5.36 ± 0.30 kJ/g PMS obtained by using acid catalyst IL. - Abstract: Paperboard mill sludge (PMS) composed of cellulose, hemicellulose, lignin and ash contents of 36.72 ± 2.81, 32.91 ± 1.75, 22.89 ± 0.56, and 7.48 ± 0.39%, respectively. Enzymatic hydrolysis process followed by fermentation of native PMS provided an ethanol yield of 0.36 ± 0.01 g/L which equivalent to net gain energy of −0.84 ± 0.03 kJ/g PMS . Choline acetate ionic liquid [Cho][OAc] IL was extensively used as a solvent for PMS to upgrade the performance. Pretreatment with [Cho][OAc] IL/PMS ratio of 10% (w/w) for 1.0 h, at a temperature of 120 °C exhibited hemicellulose and lignin removal efficiency of 5.05 ± 0.52 and 14.71 ± 1.22%, respectively with 89.19 ± 5.62% cellulose recovery. This corresponded to net gain energy of 0.60 ± 0.04 kJ/g PMS based on ethanol yield from enzymatic saccharification process which was quite low due to a limited hemicellulose removal and glucose yield of 24.1 ± 1.4 g/L. [Cho][OAc] IL/PMS ratio of 10% (w/w) supplemented with 1% (v/v) HCl substantially improved the removal efficiency of hemicellulose (36.38 ± 4.51%), lignin (17.42 ± 1.19%) and cellulose (82.17 ± 4.28%) which provided the maximum net energy of 5.36 ± 0.30 kJ/g PMS .

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013 . Scientific opinion on Dietary Reference Values for fluoride

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for fluoride, which are provided as Adequate Intake (AI) from all sources, including non-dietary sources. Fluoride is not an essential nutrient...... and adults, including pregnant and lactating women. For pregnant and lactating women, the AI is based on the body weight before pregnancy and lactation. Reliable and representative data on the total fluoride intake of the European population are not available. © European Food Safety Authority, 2013...

Mastitis Olgularında Virusların Rolü "The roles of Viruses in Mastitis"

OpenAIRE

ALPAY, Gizem; YEŞİLBAĞ, Kadir

2014-01-01

Bu derlemede özellikle sığır mastitis olgularının etiyolojisinde virusların rolleri irdelenmiştir. Viruslar sığır mastitis olgularında hem hazırlayıcı hem de primer etiyolojik ajan olarak rol oynayabilirler. Bovine herpesvirus 2, vaccinia virus, sığır çiçeği virusu, yalancı sığır çiçeği virusu, vesicular stomatitis virusu, şap hastalığı virusu ve bovine papillomavirus memede lezyon oluşturarak memenin doğal savunma mekanizmasını sekteye uğratırlar. Bovine herpesvirus 1, bovine viral diarrhoea...

Trakyada Yeşilyurt Gazetesine Göre 1944 Yılında II. Dünya Savaşı ve Kırklareli

Directory of Open Access Journals (Sweden)

Türkan Vahibe DOĞRUÖZ

2016-08-01

Full Text Available Gazeteler günlük ya da haftalık olarak, özel bir konuda veya genel konularla ilgili güncel olayları ve gelişmeleri okuyucularına ileten yazılı belgelerdir. Bu belgeler aynı zamanda yayınladıkları dönemi günümüze taşıyan birincil kaynaklardır. İl özelinde çıkan yerel gazetelerde ilde yaşanan siyasi, ekonomik, sosyal ve kültürel gelişmelere ilişkin okuyuculara ve araştırmacılara bilgi sağlarlar. Ali Rıza Dursunkaya tarafından 31 Ağustos 1925 tarihinde yayın hayatına başlayan Trakya’da Yeşilyurt gazetesi de Kırklareli’nin ilk yerel gazetesi olma özelliğini taşımaktadır. Günümüze kadar yayım hayatına devam eden gazete Kırklareli’nde yaşanan siyasi, ekonomik, sosyal ve kültürel olayları bizlere ulaştırmaya devam etmektedir. Çalışmamızda Trakya’da Yeşilyurt gazetesinin II. Dünya Savaşı’nın son yıllarına rastlayan 1944 yılına ait 07 2. Kanun (Ocak 1944- 29 2. Kanun 1944 tarihleri arasında haftada iki gün Salı ve Cuma günleri yayımlanan 1313-1413 sayıları incelenecektir. Böylece Türkiye’nin II. Dünya Savaşı sırasında yaşadığı zor günler Kırklareli örneği ile anlatılmaya çalışılacaktır. Amacımız II. Dünya Savaşı’nın yaşandığı günlerde siyasi, ekonomik, sosyal ve kültürel açıdan Kırklareli’nde yaşananları o günün gazete haberlerini kronolojik bir sıra takip ederek mevcut durumu incelemektir. Trakya’da Yeşilyurt gazetesi ile ilgili olarak böyle bir çalışmanın daha önce yapılmamış olması göz önüne alındığında dönemle ilgili Kırklareli iline yeni bir bakış açısı getireceği düşünülmektedir. İkinci Dünya Savaşı’nın son yıllarına tekabül eden ve Türkiye’nin zor zamanlarına tanıklık etmiş savaş haberleri dışında eğitim, ekonomi, tarım kooperatif, kültür, sanat, sağlık, spor, haberleriyle halka ışık tutacaktır.

Neoliberal Eğitim Politikaları ve Eğitimde Fırsat Eşitliği Bağlamında Uluslararası Sınavların (PISA, TIMSS ve PIRLS Analizi

Directory of Open Access Journals (Sweden)

Halim Güner

2014-12-01

Full Text Available Ekonomi ve eğitim ilişkisi birçok araştırmaya konu olmuş ve eğitim süreçlerinin ekonomik politikalara hizmet etmesi kabul edilen bir olgudur. Kapital sistemin oluşturduğu eşitsiz yarış sisteminin yeniden oluşturucusu yine eğitim süreçleri olarak düşünülmektedir. Eğitimin dünya genelinde belirli bir standarda ulaşıp, birçok ülkede benzer politikalar ile pazar ekonomisine hizmet edecek yapıya kavuşturulması neoliberal politikaların bir amacı olduğu düşünülmektedir. Ülkeler arasında benzer eğitim politikaları oluşturmanın etkili bir yolu günümüzde yapılan uluslararası sınavlardır. TIMSS, PISA ve PIRLS gibi sınavlar ülkelerin eğitim karnelerini ve ilişkili birçok değişkeni ülkelerin gündemine getirerek reform yapma gereksinimi algısı oluşturmaktadır. Bu reform algısının temel hedefinde ise dünya pazarında daha rekabetçi bir ekonomi için daha yarışçı bir eğitim anlayışıdır. Eğitimin insani gelişimden ziyade ekonomiye hizmet edecek donanımlı bireyler yetiştirmeye odaklanması sonucunda eşitsizlik sosyo-ekonomik duruma göre daha da derinleşmektedir. Her ne kadar uluslararası sınavların raporlarında eğitim eşitsizliğin giderilip öğrenciler arasındaki farkın kapatılması yönünde tavsiyeler barındırsa bile ülkelerin eğitim reformlarında özelleştirmeye gitmeleri ve değişimi takip eden öğretmen kalitesinin farklılık göstermesi nedeniyle öğrenci başarısındaki açı daha da büyümektedir. Bu araştırmanın amacı da bu sorunları literatür çerçevesinde işlemektir. Böylece hızlı reformların olduğu ülkemizde değişim adımlarının daha hesaplı atılmasında katkı sağlanması umulmaktadır.

Akut myeloid lösemi hastasında kronik dissemine kandidiyazisin başarılı tedavisi

OpenAIRE

K, Ozturk E; N, Soyer; S, Bayraktaroglu; M, Hekimgil; M, Tobu; B, Arda

2014-01-01

Kronik dissemine kandidiyazis (KDK) sistemik yaygın bir kandida enfeksiyonu çeşididir ve nötropenik hastaları etkiler. Bu vaka sunumunda remisyon indüksiyon kemoterapisi sırasında KDK tanısı alan ve sırasıyla amfoterisin B ve flukonazol ile tedavi edilen bir akut myeloid lösemi olgusu sunulmuştur. Kemoterapi sonrası geniş spektrumlu antibiyotiklere yanıtsız ateş ortaya çıktı. Lipozomal amfoterisin B (Lip-Amf-B) tedavisi başlandı. Serum galaktomannan (GM) testi ve kan kültürleri negatifti. Yük...

EFSA NDA Panel (EFSA Panel on Panel on Dietetic Products Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for iodine

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for iodine, which are provided as Adequate Intake (AI). Iodine is essential for the synthesis of thyroid hormones. Through these hormones, iodine...... stores in conditions of adequate iodine status before pregnancy and considers that a full compensation for the iodine secreted in breast milk is not justified for the derivation of an AI for iodine for lactating women....

Removal of colour and COD from wastewater containing acid blue 22 by electrochemical oxidation

Energy Technology Data Exchange (ETDEWEB)

Panizza, Marco [Dipartimento di Ingegneria Chimica e di Processo ' G.B. Bonino' , Universita degli Studi di Genova, p.le J.F. Kennedy 1, 16129 Genova (Italy)], E-mail: marco.panizza@unige.it; Cerisola, Giacomo [Dipartimento di Ingegneria Chimica e di Processo ' G.B. Bonino' , Universita degli Studi di Genova, p.le J.F. Kennedy 1, 16129 Genova (Italy)

2008-05-01

Electrochemical oxidation of synthetic wastewater containing acid blue 22 on a boron-doped diamond electrode (BDD) was studied, using cyclic voltammetry and bulk electrolysis. The influence of current density, dye concentration, flow rate, and temperature was investigated, in order to find the best conditions for COD and colour removal. It was found that, during oxidation, a polymeric film, causing BDD deactivation, was formed in the potential region of water stability, and that it was removed by anodic polarisation at high potentials in the region of O{sub 2} evolution. Bulk electrolysis results showed that the electrochemical process was suitable for completely removing COD and effectively decolourising wastewaters, due to the production of hydroxyl radicals on the diamond surface. In particular, under optimal experimental conditions of flow rates (i.e. 300 dm{sup 3} h{sup -1}) and current density (i.e. 20 mA cm{sup -2}), 97% of COD was removed in 12 h electrolysis, with 70 kWh m{sup -3}energy consumption.

Removal of colour and COD from wastewater containing acid blue 22 by electrochemical oxidation

International Nuclear Information System (INIS)

Panizza, Marco; Cerisola, Giacomo

2008-01-01

Electrochemical oxidation of synthetic wastewater containing acid blue 22 on a boron-doped diamond electrode (BDD) was studied, using cyclic voltammetry and bulk electrolysis. The influence of current density, dye concentration, flow rate, and temperature was investigated, in order to find the best conditions for COD and colour removal. It was found that, during oxidation, a polymeric film, causing BDD deactivation, was formed in the potential region of water stability, and that it was removed by anodic polarisation at high potentials in the region of O 2 evolution. Bulk electrolysis results showed that the electrochemical process was suitable for completely removing COD and effectively decolourising wastewaters, due to the production of hydroxyl radicals on the diamond surface. In particular, under optimal experimental conditions of flow rates (i.e. 300 dm 3 h -1 ) and current density (i.e. 20 mA cm -2 ), 97% of COD was removed in 12 h electrolysis, with 70 kWh m -3 energy consumption

Evaluation On Earthware Moulds Unearthed During The İznik Kilns Excavations / İznik Çini Fırınları Kazısı’nda Ortaya Çıkarılan Pişmiş Toprak Kalıp Parçalarının Değerlendirilmesi

Directory of Open Access Journals (Sweden)

Vesile Belgin DEMİRSAR ARLI

2018-04-01

Full Text Available A large number of mould fragments, used in the moulded ware, were found during the Iznik Kiln Excavation. Mould findings, made from red hard paste, have thick and flat bases, depressed spherical/spheroid or conical bodies, and flat spouts. Only three example among the mould fragments found is excluded from this generalization. This mould piece, have a low foot and one mould slightly flaring edge. Diameters of the spouts, the bases and heights vary between 14 and 23,6 cm, 6 and 10 cm, and 5 and 9 cm, respectively. Like in body decorations, patterns on the interiors of moulds applied in the carved and pressed technique, consist of geometrical, intricate herbal and braided compositions. These mould fragments include examples that completely overlap with the patterns we see on body fragments. The mould fragment, which consists of four connecting pieces, strikes attention with its inscriptions. However, letters visible on the obtained fragments do not represent a meaningful word, which suggests the inscription may have been intended as a decoration. Aside from these, fluting moulds constitute a separate group. These fragments have similarities to the moulds used in non-Anatolian Islamic ceramics and in Anatolia. [TR: İznik Çini Fırınları kazı çalışmaları sırasında, kalıba baskı tekniğinde kullanılan çok sayıda kalıp parçası da ele geçmiştir. Kırmızı sert hamurdan imal edilmiş kazı buluntusu kalıplar; kalın düz dipli, konik ya da basık küre gövdeli, düz ağızlıdır. Ele geçen kalıp parçaları arasında yalnızca üç örnek bu genellemenin dışında kalmaktadır. Bunlar, alçak ayaklı olup biri hafif dışa dönük kenarlıdır. Bu kalıpların ağız çapları 14-23,5 cm, dip çapları 6-10 cm, yükseklikleri 5-9 cm aralığında değişmektedir. Kalıpların iç yüzeyine oyma, kazıma ve baskı teknikleriyle uygulanan desenleri de kalıp baskı tekniğinde yapılmış gövde parçalarında olduğu gibi

Pnömatik Ekim Makinalarında Farklı Tip Dağıtma Başlıkları, Hava Hızı ve Gübreleme Normunun Akış Düzgünlüğüne Etkisi

OpenAIRE

Güler, İbrahim Ethem; Uygan, Fatih

2010-01-01

Bu araştırma pnömatik tahıl ekim makinalarında kullanılan farklı tip dağıtma başlıklarında, hava hızının ve gübreleme normunun akış düzgünlüğüne etkisini belirlemek için yapılmıştır. Araştırmada üç farklı tip dağıtma başlığı (T, Huni ve Y), üç farklı hava hızında (26, 31, 36 m/s) denenmiştir. Denemelerde Diamonyum fosfat (DAP) ve Triple süper fosfat (TSP) gübreleri kullanılmıştır. Gübreleme normları 12, 16 ve 20 kg/da, makina ilerleme hızı 1,5 m/s ve sıra arası olarak 10 cm alınmıştır. Araştı...

Türkiye’de entomofagi hakkında bir kamuoyu araştırması

OpenAIRE

CANHİLAL, Ramazan; YÜKSEL, Ebubekir

2018-01-01

Yenilebilir böcekler, gelecekte dünyanın gıda kıtlığını gidermek içiniyi bir potansiyele sahiptir ve küresel açlığı ve yetersiz beslenmeyi azaltmayayardımcı olabilirler. Entomofagi dünyanın bazı kesiminde oldukçayaygın olmasına rağmen batı ülkelerinde hala kabul görmemiştir. Türkiye’dekigenç neslin entomofagiyekarşı tutumlarını ve entomofagiyi reddetme nedenlerini belirlemek için, 2017yılında Erciyes Üniversitesi kampüsünde 610 katılımcı üzerinde bir anketçalışması yapılmıştır. Erciyes Üniver...

Effect of enzymatic treatment of extracted sunflower proteins on solubility, amino acid composition, and surface activity.

Science.gov (United States)

Conde, José Miñones; Escobar, María del Mar Yust; Pedroche Jiménez, Justo J; Rodríguez, Francisco Millán; Rodríguez Patino, Juan M

2005-10-05

Industrial proteins from agriculture of either animal or vegetable origin, including their peptide derivatives, are of great importance, from the qualitative and quantitative point of view, in food formulations (emulsions and foams). A fundamental understanding of the physical, chemical, and functional properties of these proteins is essential if the performance of proteins in foods is to be improved and if underutilized proteins, such as plant proteins (and their hydrolysates and peptides derivatives), are to be increasingly used in traditional and new processed food products (safe, high-quality, health foods with good nutritional value). In this contribution we have determined the main physicochemical characteristics (solubility, composition, and analysis of amino acids) of a sunflower protein isolate (SPI) and its hydrolysates with low (5.62%), medium (23.5%), and high (46.3%) degrees of hydrolysis. The hydrolysates were obtained by enzymatic treatment with Alcalase 2.4 L for DH 5.62 and 23.5% and with Alcalase 2.4 L and Flavorzyme 1000 MG sequentially for DH 46.3%. The protein concentration dependence on surface pressure (surface pressure isotherm), a measure of the surface activity of the products (SPI and its hydrolysates), was obtained by tensiometry. We have observed that the degree of hydrolysis has an effect on solubility, composition, and content of the amino acids of the SPI and its hydrolysates. The superficial activity and the adsorption efficiency were also affected by the degree of hydrolysis.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion related to the Tolerable Upper Intake Level of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA)

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver a scientific opinion on the Tolerable Upper Intake Level (UL) of the n-3 LCPUFAs eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA......). Available data are insufficient to establish a UL for n-3 LCPUFA (individually or combined) for any population group. At observed intake levels, consumption of n-3 LCPUFA has not been associated with adverse effects in healthy children or adults. Long-term supplemental intakes of EPA and DHA combined up...... to about 5 g/day do not appear to increase the risk of spontaneous bleeding episodes or bleeding complications, or affect glucose homeostasis immune function or lipid peroxidation, provided the oxidative stability of the n-3 LCPUFAs is guaranteed. Supplemental intakes of EPA and DHA combined at doses of 2...

Synthesis, Structures and Properties of Cu(II and Mn(II Complexes with 1,10-Phenanthroline-2-carboxylic acid and 2,2’-Bipyridine Ligands

Directory of Open Access Journals (Sweden)

Huanzhi Xu

2010-11-01

Full Text Available Four new 2,2’-bipyridine and 1,10-phenanthroline complexes, namely [Mn(phenca2]·(H2O2 (1, [Cu4(phen4(OH-4(H2O2](DMF4(ClO4-4(H2O (2, [Cu2(2,2-bipy2(C2O42-(H2O2(NO32] (3 and [Cu(2,2-bipy2(ClO4-](ClO4- (4 (2,2’-bpy = 2,2’-bipyridine, Hphenca = 1,10-phenanthroline-2-carboxylic acid have been synthesized by a hydrothermal reaction. The products were characterized by elemental analysis, infrared spectroscopy and X-ray crystal diffraction. While strong hydrogen bonds play central roles in the formation of the 3D structure, the combined influence of the weak interactions such as π···π interactions is also evident in the structures. A preliminary investigation on the ion exchange properties of the complexes is presented.

Prenatal long-chain polyunsaturated fatty acid status : the importance of a balanced intake of docosahexaenoic acid and arachidonic acid

NARCIS (Netherlands)

Hadders-Algra, Mijna

2008-01-01

This review addresses the effect of prenatal long-chain polyunsaturated fatty acid (LCPUFA) status on neuro-developmental outcome. It focuses on the major LPCUFA doxosahexaenoic acid (DNA; 22:6 omega 3) and arachidonic acid (AA; 20:4 omega 6). Due to enzymatic competition high DHA intake results in

Çocukluk çağı kabızlığında elektrolit içeren polietilen glikol kullanımı Orijinal Araştırma

OpenAIRE

Önal, Zerrin; Usta, Merve; Doğan, Yaşar; Kutlu, Tufan; Çokuğraş, Fügen; Erkan, Tülay

2008-01-01

Amaç: Bir yaşından büyük çocuklarda tedavide elektrolit içeren polietilen glikol PEG 3350 kullanımının etkinliği ve güvenilirliğini araştırmak Gereç ve Yöntem: Çalışmaya yaşları 1 ile 12 yaş arasında olan 50 olgu alındı Olguların hepsine elektrolit içeren polietilen glikol tedavisi başlandı Tedavi etkinliği olarak ağrısız yumuşak dışkılama amaçlandı Her hasta için başvuru esnasında öykü ve fizik muayeneyi içeren bilgi formu dolduruldu Tam kan sayımı karaciğer ve böbrek işlev testleri ile elek...

NDA techniques for spent fuel verification and radiation monitoring. Report on activities 6a and 6b of Task JNT C799 (SAGOR). Finnish support programme to the IAEA safeguards

Energy Technology Data Exchange (ETDEWEB)

Tarvainen, M [Finnish Centre for Radiation and Nuclear Safety, Helsinki (Finland); Levai, F [Technical Univ., Budabest (Hungary); Valentine, T E [Oak Ridge National Lab., TN (United States); Abhold, M [Los Alamos National Lab., NM (United States); Moran, B [USNRC, Washington, DC (United States)

1997-08-01

A variety of NDA methods exist for measurement of spent fuel at various stages of the disposition process. Each of the methods has weaknesses and strengths that make them applicable to one or more stages in disposition. Both passive and active methods are, under favorable conditions, capable of providing either a mapping of an assembly to identify missing fuel pins or a measurement of the fissile content and some are capable of providing a mapping of a canister to identify missing assemblies or a measurement of the fissile content. However, a spent fuel measurement system capable of making routine partial defect tests of spent fuel assemblies is missing. The active NDA methods, in particular, the active neutron methods, hold the most promise for providing quantitative measurements on fuel assemblies and canisters. Application of NDA methods to shielded casks may not be practical or even possible due to the extent of radiation attenuation by the shielding materials, and none of these methods are considered to have potential for quantitative measurements once the spent fuel cask has been placed in a repository. The most practical approach to spent fuel verification is to confirm the characteristics of the spent fuel prior to loading in a canister or cask at the conditioning facility. Fissile material tracking systems in addition to containment and surveillance methods have the capability to assure continuity of the verified knowledge of the sample from loading of the canisters to final disposal and closing of the repository. (orig.). 49 refs.

Synthesis of the Novel 4,4’- and 6,6’- Dihydroxamic - 2,2’-Bipyridines and Improved Routes to 4,4’- and 6,6’- Substituted 2,2’-Bipyridines and Mono-N-Oxide-2,2’-Bipyridine

OpenAIRE

Donnici,Claudio Luis; Máximo Filho,Daniel Henrique; Moreira,Leda Lúcia Cruz; Reis,Genuína Teixeira dos; Cordeiro,Estefania Santos; Oliveira,Ione Ma. Ferreira de; Carvalho,Sandra; Paniago,Eucler B.

1998-01-01

The preparation of key precursors for many 2,2’-bipyridine derivatives such as 4,4’-dicarboxy- 2,2’-bipyridine (I), 6,6’-dicarboxy-2,2’-bipyridine- acid (II), 4,4’-dinitro-2,2’-bipyridine-N,N-dioxide (III), 6,6’-dicarbothioamide-2,2’-bipyridine (IV) and mono-N-oxide-2,2’-bipyridine (VII) through more efficient methods is described. The syntheses of the novel ligands 4,4’-dihydroxamic-2,2’-bipyridine (V) and 6,6’-dihydroxamic-2,2’-bipyridine (VI) are also reported. Neste trabalho relatamos ...

Nematicidal Activity of Kojic Acid Produced by Aspergillus oryzae against Meloidogyne incognita.

Science.gov (United States)

Kim, Tae Yoon; Jang, Ja Yeong; Jeon, Sun Jeong; Lee, Hye Won; Bae, Chang-Hwan; Yeo, Joo Hong; Lee, Hyang Burm; Kim, In Seon; Park, Hae Woong; Kim, Jin-Cheol

2016-08-28

The fungal strain EML-DML3PNa1 isolated from leaf of white dogwood (Cornus alba L.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. The active fungal strain was identified as Aspergillus oryzae, which belongs to section Flavi, based on the morphological characteristics and sequence analysis of the ITS rDNA, calmodulin (CaM), and β-tubulin (BenA) genes. The strain reduced the pH value to 5.62 after 7 days of incubation. Organic acid analysis revealed the presence of citric acid (515.0 mg/kg), malic acid (506.6 mg/kg), and fumaric acid (21.7 mg/kg). The three organic acids showed moderate nematicidal activities, but the mixture of citric acid, malic acid, and fumaric acid did not exhibit the full nematicidal activity of the culture filtrate of EML- DML3PNa1. Bioassay-guided fractionation coupled with (1)H- and (13)C-NMR and EI-MS analyses led to identification of kojic acid as the major nematicidal metabolite. Kojic acid exhibited dose-dependent mortality and inhibited the hatchability of M. incognita, showing EC50 values of 195.2 µg/ml and 238.3 µg/ml, respectively, at 72 h postexposure. These results suggest that A. oryzae EML-DML3PNa1 and kojic acid have potential as a biological control agent against M. incognita.

Farklı Yemlerle Beslemenin Gökkuşağı Alabalığı (Oncorhynchus mykiss)’nda Hematokrit ve Hemoglobin Miktarı Üzerine Etkileri / The Effects of Liver Feed on Hematocrit and Hemoglobin Levels of Rainbow Trout (Oncorhynchus mykiss)

OpenAIRE

Atamanalp, Muhammed; Bayır, Abdulkadir; Sirkecioğlu, A. Necdet; Yanık, Telat; Yılmaz, Mehtap; Cengiz, Mehtap

2010-01-01

ÖZET: Bu araştırmada beslenme-kan parametre ilişkisini ortaya koymak amacıyla kontrollü yetiştirme şartlarında tutulan gökkuşağı alabalıkları (Oncorhynchus mykiss)’ nda rasyona karaciğer ilavesinin hematokrit ve hemoglobin değerleri üzerine etkileri belirlenmeye çalışılmıştır. Karaciğerle beslenen balıklarda gerek hemoglobin gerekse hematokrit değerleri, karaciğer + kuru karma yem ve yalnızca kuru karma yemle beslenen gruplara nazaran &...

Huzursuzlukla Yuvaya Dönüş: Fatih-Harbiye Ve Bir Tereddüdün Romanı’nda Aşılamayan Kriz

Directory of Open Access Journals (Sweden)

Jale Özata Dirlikyapan

2017-12-01

Full Text Available Döneminin en önemli romancı ve düşünce adamlarından biri olan Peyami Safa (1899-1961, hemen her devride romanları üzerine düşünülen ve söz söylenen bir yazar olmuştur. Özellikle ilk dönem romanlarının şematik yapısından sıkça söz edilmiş ve bu romanlar çoğunlukla “ulusal alegori” düşüncesi etrafında değerlendirilmişlerdir. Fatih-Harbiye ve Bir Tereddüdün Romanı kitaplarındaki kadın-erkek ilişkileri çoğunlukla bir medeniyet krizinin görünümleri olarak ele alınmış, romanlardaki bazı önemli detaylar görmezden gelinebilmiştir. Her iki romanın sonunda da Batıyı temsil eden değerler savuşturulmuş gibi görünse de, aslında romanlar bir tür bezginlik, boşvermişlik ve huzursuzlukla son bulur. Peyami Safa genelde söylendiği gibi dişil, irrasyonel, kozmopolit unsurları bünyesinden kovmayı arzular görünse de, tam da dişil, irrasyonel veya “vahşi ölçüde samimi” olanın alanındayken kısmen daha özgür bir bilinçle nitelikli edebiyat yapıyordur. Birbirine sıkı sıkıya bağlı olan bu unsurlar, yazarın “milli ideal” etrafında ördüğü kürsü konuşmaları tarafından gizlenmeye, baskılanmaya çalışılsa da, bunları ele alışı sayesinde Peyami Safa’daki “büyük romancı” kendini gösterebilmiştir

Effect of supplementation of arachidonic acid (AA) or a combination of AA plus docosahexaenoic acid on breastmilk fatty acid composition

NARCIS (Netherlands)

Smit, EN; Koopmann, M; Boersma, ER; Muskiet, FAJ

We investigated whether supplementation with arachidonic acid (20:4 omega 6; AA), ora combination of AA and docosahexaenoic acid (22:6 omega 3; DHA) would affect human milk polyunsaturated fatty acid (PUFA) composition. Ten women were daily supplemented with 300 mg AA, eight with 300 mg AA, 110 mg

Kıl Keçi ve Saanen x Kıl Keçi Melezlerinin (F1, G1, Çiftçi Şartlarında Süt Verim Özellikleri Bakımından Karşılaştırılması

Directory of Open Access Journals (Sweden)

Hilal Tozlu Çelik

2014-11-01

Full Text Available Bu araştırma, Amasya ili Sarılar köyü, özel bir işletmede 2011-2012 yılları arasında yetiştirilen Saanen x Kıl keçi melezleri (F1, G1 ve Kıl keçilerinde süt verimi özellikleri üzerine genotip, yıl ve yaş faktörlerinin etkisini ortaya koymak amacıyla yapılmıştır. Araştırmada 2011 ve 2012 yıllarında günlük ortalama süt verimi (GOSV, laktasyon süresi (LS ve laktasyon süt verimi (LSV üzerine genotipin etkili olduğu bulunmuştur. 2011 yılında yaşın tüm genotiplerde GOSV ve LSV etkisinin olduğu tespit edilmiştir. Araştırmada 2012 yılında yaşın tüm genotiplerde GOSV ve LS'ne etkisinin olduğu saptanmıştır. F1 genotipinde ve Kıl keçilerde yılın GOSV, LSV ve LS üzerine etkili olduğu bulunmuştur. G1 genotipinde yılın sadece LS üzerine etkisi olduğu saptanmıştır. Sonuç olarak çiftçi şartlarında yetiştirilen Saanen x Kıl keçi F1 ve G1 genotipinin süt verim özelliklerinin Kıl keçilerden daha yüksek olduğu söylenebilir.

Biological activity of some novel synthesized 2-(4-methylbenzenesulphonamidopentanedioic acid bis amide derivatives: In vitro and in vivo antineoplastic activity

Directory of Open Access Journals (Sweden)

Satyajit Dutta

2014-12-01

Full Text Available In the present work few novel 2-(4-methylbenzenesulphonamidopentanedioic acid bis amide derivatives and the basic compound 2-(4-methylphenylsulfonamidopentanedioic acid have been synthesized, characterized and screened for their possible antineoplastic activity both in vitro and in vivo. The in vitro activity was performed against five human cell lines like human breast cancer (MCF-7, leukemia (K-562, ovarian cancer (OVACAR-3, human colon adenocarcinoma (HT-29 and Human kidney carcinoma (A-498. The in vivo activity was performed in female swiss albino mice against Ehrlich ascites carcinoma (EAC. Among the synthesized compounds, ureide, anilide, p-nitoanilide and o-bromoanilide derivatives of 2-(4-methyl benzene sulphonyl-pentanedioic acid bis amides showed encouraging activity in both the in vitro and in vivo compared to other compounds.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for zinc

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values for zinc, using a two-stage factorial approach and reference values for body weight. The first stage of estimating physiological requirements used studies...... from 2.4 to 11.8 mg/day. PRIs for infants and children were derived by assuming a coefficient of variation of 10 %, and range from 2.9 to 14.2 mg/day. For pregnancy and lactation, additional zinc requirements related to fetal and maternal tissues and transfer of zinc into breast milk, respectively...

Kobi niteliğindeki sağlık kuruluşlarında iç kontrol sistemi ve önemi

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Güler GÜNEY

2014-06-01

Full Text Available Büyük küçük tüm sağlık kuruluşlarının, sürekliliklerini sağlayabilmeleri ve hedeflerine ulaşabilmeleri bakımından en önemli kriter etkili bir iç kontrol sistemine sahip olmalarıdır. Özellikle Türkiye gibi ekonomisi gelişmekte olan, ticari aktörlerinin çok büyük bir kısmı KOBİ statüsünde olan ülkelerde, ekonomik hayatı hareketlendirmek, sermaye piyasalarının gelişmesi ve işlerliğinin kuvvetlenmesinin sağlanmasında da yine en temel kriter etkin ve etkili bir iç kontrol siteminin varlığıdır. Çünkü etkili bir iç kontrol sistemi; hata, hile ve yolsuzlukların önlenmesinde ve işletme içinde uygulanan politika ve prosedürlerin uygulanma düzeyi ve verimliliğinin sağlanmasında da önemli bir etkendir. İster büyük, ister küçük olsun her işletmede etkin olan ya da olmayan bir iç kontrol mevcuttur. Ancak bu kontrollerin işletmede belirlenmiş bulunan belli politika ve prosedürlere göre yapılıp yapılmadığı ya da etkin olup olmadıkları tartışılabilmektedir. Ülkemizdeki sağlık kuruluşlarından, özellikle sermaye piyasalarında işlem gören kuruluşlarda mevzuatlar gereği iç kontrol sistemi kurulmuş olmasma rağmen, bu iç kontrol sisteminin tamamıyla örgütsel amaçlar doğrultusunda etkili ve verimli şekilde çalıştığını söylemek mümkün değildir. Ülkemizdeki KOBİ niteliğindeki sağlık kuruluşlarının hatırı bir kısmında ise iç kontrol sisteminin oldukça yetersiz ve zayıf olarak nitelenebilecek şekilde uygulandığı ifade edilebilir. Bu da diğer tüm KOBÎ'lerde olduğu gibi sağlık alanındaki kuruluşların büyüme, gelişme ve kurumsallaşması üzerine olumsuz etkide bulunmaktadır. Daha da önemlisi de kurumların örgütsel amaçlarını gerçekleştirmesinde zorluklarla karşılaşmasına sebep olmaktadır. Başka bir deyişle, etkin iç kontrol sisteminin işletmelerin ilgililerine fayda sağlaması gereklidir. Böylece i

An efficient process for lactic acid production from wheat straw by a newly isolated Bacillus coagulans strain IPE22

DEFF Research Database (Denmark)

Zhang, Yuming; Chen, Xiangrong; Luo, Jianquan

2014-01-01

features, an efficient process was developed to produce LA from wheat straw. The process consisted of biomass pretreatment by dilute sulfuric acid and subsequent SSCF (simultaneous saccharification and co-fermentation), while the operations of solid–liquid separation and detoxification were avoided. Using...... this process, 46.12 g LA could be produced from 100 g dry wheat straw with a supplement of 10 g/L corn steep liquid powder at the cellulase loading of 20 FPU (filter paper activity units)/g cellulose. The process by B. coagulans IPE22 provides an economical route to produce LA from lignocellulose...

Synthesis and characterization of bisdiglycolamides for comparable extraction of Th"4"+, UO_2"2"+ and Eu"3"+ from nitric acid solution

International Nuclear Information System (INIS)

Peng Ren; Ze-Yi Yan; Yang Li; Zuo-Miao Wu; Lei Wang; Yi-Quan Gao; Wang-Suo Wu; Lian-Biao Zhao

2017-01-01

The novel ligand N,N,N'''',N''''-tetrabutyl-N''',N'''-(N',N'-diethyl)-ethidene bisdiglycolamide (TBEE-BisDGA) and other eight analogous extractants have been synthesized and characterized by NMR and HRMS. The solvent extraction of Th"4"+, UO_2"2"+ and Eu"3"+ from nitric acid solution using the above BisDGA extractants was investigated in 1-dodecanol at 30 ± 1 deg C. The extractants exhibited higher affinity toward Th"4"+ than UO_2"2"+ and Eu"3"+ in the present system. The maximum value of separation factor SF_T_h_(_I_V_)_/_U_(_V_I_) and SF_T_h_(_I_V_)_/_E_u_(_I_I_I_) is 78.5 and 53.3 respectively for TBEE-BisDGA, 88.1 and 69.5 respectively in the case of TBi-PE-BisDGA at 3 M HNO_3 solution. (author)

Trichoderma harzianum T-22 induces systemic resistance in tomato infected by Cucumber mosaic virus

Directory of Open Access Journals (Sweden)

Antonella Vitti

2016-10-01

Full Text Available Understanding the induction of plant defenses against viruses using biocontrol agents is essential for developing new strategies against these pathogens, given the ineffectiveness of chemical treatments. The ability of Trichoderma harzianum, strain T-22 (T22 to control Cucumber mosaic virus (CMV in Solanum lycopersicum var. cerasiforme plants and the changes in the physiology of tomato treated/infected with T22/CMV were examined. Plant growth-promoting effects, photosynthetic performance, reactive oxygen species (ROS scavenging enzymes, and phytohormones were investigated. T22 improved tomato growth in terms of plant height and improved photosynthesis, total chlorophyll content and plant gas exchange. In contrast, CMV induced a negative effect on dry matter accumulation and inhibited the photosynthetic capacity. The analysis of plant hormones demonstrated that treating with T22 before or simultaneously to CMV infection, led to a systemic resistance by jasmonic acid/ethylene and salicylic acid signaling pathways. Conversely, systemic resistance was abscissic acid-dependent when T22 treatment was administered after the CMV infection. In conclusion, the data reported here indicate that the T22-based strategy may be the most effective measure against CMV.

Performance results from the first integrated NDA VXI safeguards system

International Nuclear Information System (INIS)

Bot, D.; Messner, R.

1999-01-01

The VIFM system is the first integrated safeguard system to be developed and deployed by the IAEA. The system is also noteworthy in that it is the most versatile of any NDA system thus far deployed. Due to the high level of functionality, the VIFM is probably the most 'tested' of all IAEA systems. This paper discusses the results obtained from detailed in field and laboratory testing of a variety of VIFM configurations ranging from simple single systems to highly integrated system implementations. A total of 4 sites have had VIFM equipment installed. Data collected from these sites have been of very high quality and consistency despite the failure of commercial hard disk drive equipment. The systematic failure of these drives have been corrected using a variety of methods and performance since those corrections have been excellent with no equipment failures. The tests carried out also included the test of a twisted pair to coaxial cable interface. This interface was required in order to allow installation even with a significant facility cabling limitation. The performance obtained from the system utilizing this device showed no degradation as compared to that of systems utilizing direct coaxial cable connections. To conclude, the VIFM system, has been reliable even after partial failure of commercial off-the-shelf components which required the systems to operate on full fail-safe backup. These tests have thus shown both the reliable operation of the VIFM in normal operating conditions as well as the most adverse

Design, docking, synthesis and anticancer activity of some novel 2-(4-methylbenzenesulphonamidopentanedioic acid amide derivatives

Directory of Open Access Journals (Sweden)

Satyajit Dutta

2014-08-01

Full Text Available In the present work few novel 2-(4-methylbenzenesulphonamidopentanedioic acid amide derivatives and the basic compound 2-(4-methylphenylsulfon-amidopentanedioic acid have been designed, synthesized, characterized and screened for their possible antineoplastic activity both in vitro and in vivo. The modified drugs were docked against the protein histone deacetylase the energy value obtained was o-iodoanilide (-10.370504 and m-iodoanilide (-10.218276 of the titled compound. The in vitro activity was performed against five human cell lines like human breast cancer (MCF-7, leukemia (K-562, ova-rian cancer (OVACAR-3, human colon adenocarcinoma (HT-29 and Human kidney carcinoma (A-498. The in vivo activity was performed in female Swiss albino mice against Ehrlich Ascites Carcinoma (EAC. Among the synthesized compounds, o-iodoanilide, m-iodoanilide and p-iodoanilide derivatives of 2-(4-methyl benzene sulphonyl-pentanedioic acid amides showed encouraging activity in both the in vitro and in vivo compared to other compounds.

Can Intestinal Fatty Acid Binding Protein (I-FABP Be A Marker in the Diagnosis of Abdominal Pathology?

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Ozlem UZUN

2014-09-01

Full Text Available SUMMARY: Objectives: Biochemical markers play an important role in the early diagnosis of abdominal pain. This study aimed to investigate the diagnostic value of intestinal type fatty acid binding protein (I-FABP in patients with abdominal pathology. Methods: This prospective and descriptive study was performed at the University Hospital Emergency Department. Serum I-FABP levels of patients presenting with acute abdominal pain were measured at time of admission and were compared with those of healthy individuals. Results: The mean I-FABP level of the 171 patients enrolled in this study was 170.1±543.4 pg/ml, while that of a healthy control group was 61.4±47.4 pg/ml. Although I-FABP levels were higher in the patient group, this difference was not statistically significant (p>0.05. However, I-FABP levels of patients with mesenteric ischemia and intra-abdominal mass were significantly higher than those of healthy individuals (p≤0.05. Conclusions: I-FABP levels that are evaluated at time of admission in patients presenting with abdominal pain to the emergency department are significantly higher in patients with mesenteric ischemia and intra-abdominal mass than are those of healthy individuals. ÖZET: Amaç: Biyokimyasal belirteçler karın ağrısının sebebinin erken tanısında oldukça önemlidir. Bu çalışmada FABP'nin intestinal tipinin (I-FABP abdominal patolojisi olan hastaların ayırıcı tanısındaki değeri araştırıldı. Gereç ve Yöntem: Mevcut çalışma üniversite hastanesi acil servisinde ileriye yönelik tanımlayıcı olarak çalışma gerçekleştirildi. Karın ağrısı şikayeti ile acil servise başvuran hastalarda başvuru anında alınan serum örneklerinden I-FABP değerlerine bakıldı. Bulgular sağlıklı gönüllülerdeki düzeyler ile karşılaştırıldı. Bulgular: Kayıtlı 171 hastada ölçülen ortalama I-FABP seviyesi 170.1±543.4 pg/ml olarak belirlendi. Sağlıklı gönüllülerde I-FABP seviyesi 61

Siyaset ve Apolitizm Arasında Mısır’da Selefi Hareket: ed-Da’vetu’s-Selefiyye Örneği

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Nurullah ÇAKMAKTAŞ

2015-09-01

Full Text Available İslam’ı anlama ve yorumlama yöntemlerinden biri olarak Selefilik, geçmişi İslam tarihinin ilk dönemlerine kadar uzanan bir düşünce ekolüdür. Fakat 20. yy’da İslam dünyasında kurulan ulus devletler içinde ise Selefilik, bu düşüce ekolü olma özelliğinin yanında İslami hareket bünyesine giren bir karakter de kazanmıştır. Özellikle 11 Eylül saldırılarının akabinde aceleye getirilen analizler sonucu şiddet ile özdeşleştirilen Selefiliğin içinde, siyasal mücadeleyi ve şiddeti bir değişim yöntemi olarak kabul etmeyen ve çok daha büyük toplumsal desteğe sahip selefi hareketler bulunmaktadır. Eğitim, irşat ve davet yöntemleri ile toplumun ve devletin İslamlaşmasını savunan söz konusu bu apolitik selefi hareketlerin büyüklüğünün ve etkinliğinin tam anlamıyla farkına ancak Arap devrimleri sonrası varılabilmiştir. Biz de bu araştırmamızda, söz konusu sürecin ön plana çıkardığı apolitik bir selefi hareket olan, 25 Ocak Devrimi sonrasında siyasallaşan ve fakat Temmuz 2013’te gerçekleşen askeri darbenin akabinde tekrardan karakteristik özellikleri olan apolitikliğe geri döndüğünü düşündüğümüz ed-Da’vetu’s-Selefiyye grubunu; ortaya çıkışı, yapısı, faaliyet alanları, düşünce esasları, siyaset düşüncesi, benimsediği yöntem ve yaşadığı dönüşümler çerçevesinde Mısır özelinde incelemeye çalıştık

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific Opinion on Dietary Reference Values for cobalamin (vitamin B12)

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for cobalamin (vitamin B12). The Panel considers that the approach based on a combination of biomarkers of cobalamin status, i.e. serum....... For pregnancy and lactation, additional cobalamin intakes related to the accumulation of cobalamin in fetal tissues and transfer of cobalamin into breast milk are considered and AIs of 4.5 and 5 μg/day, respectively, are proposed....

Interactive effects of glutamine and gamma-aminobutyric acid on growth performance and skeletal muscle amino acid metabolism of 22-42-day-old broilers exposed to hot environment

Science.gov (United States)

Hu, Hong; Bai, Xi; Shah, Assar Ali; Dai, Sifa; Wang, Like; Hua, Jinling; Che, Chuanyan; He, Shaojun; Wen, Aiyou; Jiang, Jinpeng

2016-06-01

The present experiment was conducted to investigate the interactive effects between dietary glutamine (Gln, 0 and 5 g/kg) and gamma-aminobutyric acid (GABA, 0 and 100 mg/kg) on growth performance and amino acid (AA) metabolism of broilers under hot environment. A total of 360 22-day-old Arbor Acres male chickens were randomly assigned to five treatment groups under thermoneutral chamber (PC, 23 °C) and cyclic heat stress (HS, 30-34 °C cycling) conditions. Compared with the PC group, cyclic HS decreased ( P muscle at 28, 35, and 42 days, while it increased ( P muscle, the Gln supplementation increased ( P muscle Gln concentrations, glutaminase activities, GS activities at 28 and 35 days, and DWG, GABA concentrations, and GABA-T activities at 28, 35, and 42 days in broilers under cyclic HS. In conclusion, the present results indicated that the interactions of exogenous Gln and GABA could offer a potential nutritional strategy to prevent HS-related depression in skeletal muscle Gln and GABA metabolism of broilers.

IAEA experience with authentication of in-plant NDA instrumentation

International Nuclear Information System (INIS)

Augustson, R.H.; Dermendjiev, E.

1983-01-01

The paper discusses IAEA experience with permanently installed measuring equipment, i.e. in-plant NDA instrumentation, which often has advantages over portable equipment, such as improved accuracy, automated sample handling and data collection, and capacity for higher throughput. In some cases, in-plant equipment is the only means of making a field measurement. However, the use of in-plant equipment requires an additional set of inspector procedures to ensure that the instrument is working correctly and has not been tampered with. This process of verifying instrument performance is called authentication. General guidelines for approaches to authentication have been studied and formulated by an IAEA Advisory Group Meeting held in November 1981. Procedures for specific instruments have been developed in some cases with the help of national support programmes. The field application of authentication is accomplished by incorporating specific actions into inspection procedures. Results are written down as part of the working papers and included in the final inspection report. For quantitative checks such as measurement of a working standard the results are sent along with the inspection measurements to the Agency for inclusion in the safeguards data base. The in-plant equipment may be owned by the facility, a State, a safeguards organization or the Agency. In each case, the use of the in-plant equipment will necessitate additional interactions between facility operator and inspector, in order to judge the impact on plant operation, and understand what is being measured and what can go wrong. The paper discusses the IAEA's experience gained in the field application of authentication procedures for instrument systems such as weighing and volume measuring devices, rod scanners, neutron activation systems and K-edge densitometers

An irresolute linker: separation, and structural and spectroscopic characterization of the two linkage isomers of a Ru(ii)-(2-(2'-pyridyl)pyrimidine-4-carboxylic acid) complex.

Science.gov (United States)

Iengo, E; Demitri, N; Balducci, G; Alessio, E

2014-08-28

For the first time the two linkage isomers of a Ru(ii) complex with 2-(2'-pyridyl)pyrimidine-4-carboxylic acid (cppH) - that form in comparable amounts - have been fully characterized individually. The X-ray structure of each isomer is related to its NMR spectrum in solution.

Çocuk Kitaplarında Mülteciler ve Kültürleşme Stratejileri- Refugees and Acculturation Strategies in Children’s Books

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Elif Emine BALTA

2018-04-01

Full Text Available İnsanın veya insanların sosyal, ekonomik, siyasi veya başka sebeplerden dolayı yaşadıkları yerden farklı bir yere doğru hareket etmesi veya yerleşmesi olarak tanımlanabilen göç, insanın varoluşundan bu yana olan bir hadisedir. Göç, ister ilkel ister günümüzdeki örnekleri gibi karmaşık olsun insanların daha iyiyi arama bulma çabasından kaynaklanmaktadır. Buna rağmen göçe karar verme, göç süreci ve sonrasında yeniye alışma ve uyum sağlama zorluklarla doludur. Çocuğun küresel dünyanın bir parçası olması, toplumsal sorunların farkında olması, farklı insan, yaşayış ve kültürlerin var olabileceğinin bilincinde olması ve önyargılarından arınması için sosyal problemlerin örnekleriyle karşılaşması gerekmektedir. Türk çocuk edebiyatında son birkaç yılda mülteci ve sığınmacı göçleri ile ilgili konuların işlendiği görülmektedir. Bu çocuk kitapları, mültecilerin toplumsal kabul görmeleri noktasında düşünülerek yazılmış, toplumda farkındalık yaratmayı amaçlayan bir anlamda güdümlü eserler olarak karşımıza çıkmaktadır. Çocukların empati yapabilmelerine imkân tanıyacak eserlerin varlığı bu bağlamda sevindiricidir. Bu çalışmada, Umut Sokağı Çocukları, Kömür Karası Çocuk, Kuş Olsam Evime Uçsam, Juju Beni Unutma, Halepli Zeynep adlı kitaplar kültürleşme modeli perspektifinden değerlendirilmiştir. Türk toplumunun, özellikle çocukların vicdanına seslenen bu kitapların ortak noktasının mültecileri anlamak için çaba göstermeye odaklanma olduğu söylenebilir. Mültecilerin göç etme ve Türkiye’ye uyum sağlama süreçlerinde yaşadıkları, duygu ve düşünceleri çocuk bakış açısıyla sunulmuştur. Bununla birlikte Türk halkının mültecilere tavrının ve bu tavrın çocuk ruhuna yansımasının gerçekçi biçimde aktarıldığını söylemek mümkündür.

Data evaluation for operator-inspector differences for a specific NDA instrument

International Nuclear Information System (INIS)

Franklin, M.

1984-01-01

The Joint Research Centre (JRC) of the European Commission is developing a number of NDA instruments for safeguards use. In particular the JRC has developed a photo neutron active interrogation device (Phonid) for the assay of U-235 in bulk quantities. This report describes new statistical results for the D statistic in the context of data evaluation algorithms for the Phonid instrument. The Phonid instrument is useful for this purpose because its error propagation structure is well characterised and yet not trivially simple. The data evaluation for Phonid data is derived from its error propagation modelling plus new results for the sampling distribution of the D statistic. The problem of assigning an uncertainty to the D statistic value without any diversion strategy assumptions has long been an unresolved problem. The results described in this report provide the solution to this problem by considering the sampling distribution of the D statistic given the population of discrepancies. Discrepancy is defined as the difference between operator declared values and the true values measured by the inspector. This approach provides estimable expressions for the sampling moments of the D statistic without making any assumption about the cause (diversion, clerical error, measurement error) of the discrepancy. The report also provides a general discussion of the distinction between planning a verification and performing the data analysis after the verification has been carried out

Sinema-Resim İlişkisi Bağlamında David Lynch ve ‘Silgikafa’ Filmi

OpenAIRE

Güngör, Arif Can

2016-01-01

Sinema geçmişten günümüze kadar tüm sanat dallarından yararlanmış, özellikle görsel bir sanat olan resim sanatıyla estetik açıdan çok yakın bir ilişki içinde bulunmuştur. Sinemanın resim sanatından yararlanmasında en önemli etken yönetmenin bu sanatla kurduğu bağ olmuş, özellikle ressam yönetmenler estetik anlamda resmin olanaklarından yararlanarak filmlerine plastik bir değer katmışlardır. Bu makalenin amacı sinema sanatı ile resim sanatı arasındaki ilişkiyi ressam yönetmen David Lynch ve on...

Topraktan Pestisitlerin Arındırılmasında Önemli Bir Araç: Fitoremediasyon

OpenAIRE

TİRYAKİ, Osman; POTUR, Tayfun

2018-01-01

Pestisitler bitki koruma önlemleri arasındaen fazla uygulananıdır.Ancak, pestisitlerin tarımsal alanlarda bilinçsiz ve yoğun bir şekilde kullanımı bazı ekosistem problemlerine neden olur.Pestisitlerin sınıflandırma şekillerinden biri de topraktaki yarılanma sürelerine göre yapılanıdır. Bu sınıflandırmada pestisitler kalıcı değil, orta derece kalıcı, kalıcı ve devamlı kalıcı olarak 4 gruba ayrılırlar. Kullanılan pestisitler arasında toprakta çok çabuk parçalananları olduğu gi...

Caracterização termogravimétrica e espectroscópica das propriedades ácidas da zeólita ZSM-22 Thermogravimetric and spectroscopic characterization of acidic properties of ZSM-22 zeolite

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A. R. Loiola

2010-07-01

Full Text Available A acidez da estrutura da zeólita ZSM-22 foi determinada por análise térmica. A energia de ativação, calculada pelo método de Ozawa, envolveu uma energia de termodecomposição de 18,44 kJ.mol-1. O valor do fator pré-exponencial A foi de 2,2746. Suas propriedades texturais foram determinadas por análise de dessorção/adsorção de nitrogênio, cujos resultados indicaram a existência de microporosidade, com área de superfície de 257 m².g-1, volume de microporos de 0,041 cm³.g-1 e diâmetro de poros de 32,6 Å. Análises de luminescência, utilizando európio como sonda, sugerem que a acidez é distribuída uniformemente dentro da estrutura da zeólita.The acidity and stability of ZSM-22 zeolite structure have been studied by thermal analysis. The activation energy calculated by Ozawa method to the events related to thermodecomposition was 18.44 kJ.mol-1. The value of the pre-exponential factor A was 2.2746. Its textural properties have been determined by nitrogen adsorption desorption analysis, whose results indicated microporous properties, with surface area 257 m².g-1, micropore volume 0.041 cm³.g-1 and pore diameter 32.6 Å. Luminescence analyses, using europium as probe, suggest that the acidity is distributed uniformly within the zeolite structure.

Translations on Eastern Europe, Scientific Affairs, No. 562

Science.gov (United States)

1977-10-28

achievements in the field of molecular biology we can cite such achievements as establishment of the composition and structure of nucleic acids and...way on study of the dynamics of metabolite processes, the biosynthesis of antibiotics, alka- loids, organic acids and protein substances, as well as...nitrocellulose in 1062, celluloid in 1070, Bakelite in 1872, urea formaldehyde resins in 1918, methyl polymethacrylate in 1929-1931, and Melamine in 193*t

BIOASSAY STUDIES OF METAL(II) COMPLEXES OF 2,2'-(ETHANE ...

African Journals Online (AJOL)

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diyldiimino)diacetic acid (EDDA) were prepared and characterized. Coordination complexes of the EDDA ... corresponding amines with alkyl halide to bear diammines of the same class with different substituents. ... Bioassay studies of metal(II) complexes of 2,2'-(ethane-1,2-diyldiimino)diacetic acid. Bull. Chem. Soc. Ethiop.

[Acids in coffee. XI. The proportion of individual acids in the total titratable acid].

Science.gov (United States)

Engelhardt, U H; Maier, H G

1985-07-01

22 acids in ground roast coffees and instant coffees were determined by GLC of their silyl derivatives (after preseparation by gel electrophoresis) or isotachophoresis. The contribution to the total acidity (which was estimated by titration to pH 8 after cation exchange of the coffee solutions) was calculated for each individual acid. The mentioned acids contribute with 67% (roast coffee) and 72% (instant coffee) to the total acidity. In the first place citric acid (12.2% in roast coffee/10.7% in instant coffee), acetic acid (11.2%/8.8%) and the high molecular weight acids (8%/9%) contribute to the total acidity. Also to be mentioned are the shares of chlorogenic acids (9%/4.8%), formic acid (5.3%/4.6%), quinic acid (4.7%/5.9%), malic acid (3.9%/3%) and phosphoric acid (2.5%/5.2%). A notable difference in the contribution to total acidity between roast and instant coffee was found for phosphoric acid and pyrrolidonecarboxylic acid (0.7%/1.9%). It can be concluded that those two acids are formed or released from e.g. their esters in higher amounts than other acids during the production of instant coffee.

A Subpopulation of the K562 Cells Are Killed by Curcumin Treatment after G2/M Arrest and Mitotic Catastrophe.

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Macario Martinez-Castillo

Full Text Available Curcumin is extensively investigated as a good chemo-preventive agent in the development of many cancers and particularly in leukemia, including treatment of chronic myelogenous leukemia and it has been proposed as an adjuvant for leukemia therapies. Human chronic myeloid leukemia cells (K562, were treated with 20 μM of curcumin, and we found that a subpopulation of these cells were arrested and accumulate in the G2/M phase of the cell cycle. Characterization of this cell subpopulation showed that the arrested cells presented nuclear morphology changes resembling those described for mitotic catastrophe. Mitotic cells displayed abnormal chromatin organization, collapse of the mitotic spindle and abnormal chromosome segregation. Then, these cells died in an apoptosis dependent manner and showed diminution in the protein levels of BCL-2 and XIAP. Moreover, our results shown that a transient activation of the nuclear factor κB (NFκB occurred early in these cells, but decreased after 6 h of the treatment, explaining in part the diminution of the anti-apoptotic proteins. Additionally, P73 was translocated to the cell nuclei, because the expression of the C/EBPα, a cognate repressor of the P73 gene, was decreased, suggesting that apoptosis is trigger by elevation of P73 protein levels acting in concert with the diminution of the two anti-apoptotic molecules. In summary, curcumin treatment might produce a P73-dependent apoptotic cell death in chronic myelogenous leukemia cells (K562, which was triggered by mitotic catastrophe, due to sustained BAX and survivin expression and impairment of the anti-apoptotic proteins BCL-2 and XIAP.

1,1′-Binaphthyl-2,2′-dicarboxylic acid–urea (1/1

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Edwin Weber

2008-10-01

Full Text Available In the title co-crystal, C22H14O4·CH4N2O, the 1,1′-binaphthyl-2,2′-dicarboxylic acid (BNDA and urea molecules are connected via a system of hydrogen bonds into a chiral two-dimensional polymeric structure parallel to the (001 plane. As the crystal is centrosymmetric, it consists of alternately stacked BNDA–urea layers of opposite chirality. The urea H atoms trans to the C=O group are bonded in a chelating mode [R12(6] to the carbonyl O atom from one of the carboxylic acid groups which, in turn, acts as the donor of an O—H...O hydrogen bond to another urea molecule. The [010] chains thus formed are further connected via an R22(8 hydrogen-bond motif formed between urea and the second carboxylic acid group of BNDA.

12 Mart Romanlarında Kadın Tanımlamaları The Caharacterization Of Women In The March Twelfth Novels

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Ahmet ALVER

2012-12-01

Full Text Available It is estimated that between 1970 and 1980 more than 5,000 people died in politically motivated violence in Turkey. The ‘March 12th novels’ is a retroactive umbrella term used to describe the works of fiction written this period. Their inspiration is the events surrounding the March 12th military intervention; the military takeover, the battle between revolutionary left wing activists and nationalists, and the political and social fallout of the intervention. It is during this period of persecution and oppression that the March 12th novels were written and they provide an invaluable source for anyone wanting to understand the effects of the persecution and atrocities committed by the state on its people.This article examines the characterization of women in 1970s Turkey through number of March 12 novels written by particularly female novelists. It studies the characterization of revolutionary women; their treatment by wider society, and the price they paid for being revolutionary. Along with revolutionary women, the article also focuses other examples of female figures such as bourgeois women who are presented as perfect consumers willing to accept subjugation to the patriarchy in exchange for material wealth, whilst, on the other hand, rural women are presented as ignorant and almost bestial creatures who view the female body as nothing more than a vehicle for reproduction and the satisfaction of men.This article in particular has focused upon works such as 47”liler (The Generation of ’47 written by Füruzan, Şafak (The Dawn by Sevgi Soysal, Bir Düğün Gecesi (A Wedding Night by Adalet Ağaoğlu and Yarın Yarın (Tomorrow Tomorrow by Pınar Kür. Türkiye’de, 1970 ve 1980 yılları arasında, siyasi sebeplerle meydana gelen şiddet olaylarında değişik kaynaklara göre sağ ve sol görüşlü 5.000 dolayında vatan evladının hayatını kaybettiği tahmin edilmektedir. 12 Mart 1971 askeri müdahalesinin öncesi ve sonrasında

Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245.

Science.gov (United States)

Sayed, Mahmoud; Dishisha, Tarek; Sayed, Waiel F; Salem, Wesam M; Temerk, Hanan A; Pyo, Sang-Hyun

2016-03-10

Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245 as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl)butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5:5:2 was found optimum. The optimal conditions for biotransformation were pH 8, 1v/v/m airflow and 500rpm stirring speed. In batch mode of operation, 70.6% of 5g/l TMP was converted to BHMB in 10 days. For recovery of the product the adsorption pattern of BHMB to the anion exchange resin, Ambersep(®) 900 (OH(-)), was investigated in batch and column experiments giving maximum static and dynamic binding capacities of 135 and 144mg/g resin, respectively. BHMB was separated with 89.7% of recovery yield from the fermentation broth. The approach is applicable for selective oxidation of other highly branched polyols by biotransformation. Copyright © 2016 Elsevier B.V. All rights reserved.

Guidance on the scientific requirements for health claims related to gut and immune function

DEFF Research Database (Denmark)

Tetens, Inge

2011-01-01

The European Food Safety Authority (EFSA) asked the Panel on Dietetic Products Nutrition and Allergies (NDA) to draft guidance on scientific requirements for health claims related to gut and immune function. This guidance has been drawn from scientific opinions of the NDA Panel on such health......, was subjected to public consultation (28 September 2010 to 22 October 2010), and was also discussed at a technical meeting with experts in the field on 2 December 2010 in Amsterdam....

Esrâr Dede Divanı’nda Renkler Colours In Dıvan Of Esrâr Dede

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Mahmut KAPLAN

2013-03-01

compliance with their frequency of usage in the work.The article has been completed with a general evaluation. Esrâr Dede, klasik şiirimizin önemli simalarından biridir. Mevlevîliğe intisap ettikten sonra şiire başlamış, önemli eserler meydana getirmiştir. Şeyh Galib’in dostluğunu kazanmıştır. Şairin divanı dışında tezkiresi ve lügati bulunmaktadır. Esrar Dede, duygu ve düşüncelerini anlatmak için renk ifade eden kelimeleri sık kullanmıştır. Renk kelimeleri kültürel mirasın da önemli taşıyıcılarıdır. Varlıkları tasvir ve tarifte kullanılan renkler toplumun bazı metinlerde sembolik anlamlar da kazanır. Türkçede bazı renklerin yön bildirdiği bilinmektedir. Renkler bazen duyguları anlatmak için kullanılır, kişinin ruh hallerini ifade ederler. Tabiat manzaralarını tasvir etmek için renkler şairlerin elindeki en önemli araçlardır. Şair, tabiatı renk kelimeleri ile gözler önüne serer, renkler vasıtasıyla duygu ve düşüncelerini sembolik ifadelerle dile getirir. Kasidelerin nesiplerinde yapılan tasvirlerde renklerin önemli görevleri vardır. Çoğu gazelleri âşıkane olan Esrar Dede de duygu ve düşüncelerini anlatmak için renk sıfatlarına başvurmuştur. Esrar Dede Divanı’nda özellikle siyah ve kırmızı hâkim renkler olarak karşımıza çıkmaktadır. Bu renkler varlıkları tasvir etmek ve tasavvufî bir takım durumları anlatmak için kullanılmıştır. Kara, genel olarak gece, saç ve karanlık tasvirlerinde karşımıza çıkar. Ayrıca kara baht, talihsizlik gibi durumları tasvir etmede bu renge başvurulur. Kırmızı, gül, şafak, kan, dudak tasvirlerinde karşımıza çıkar. Bu iki temel renk dışında beyaz, sarı, yeşil ve tasavvufî durumları ifade eden renksizlik, bî-renklik şairin kullandığı renkler arasında yer almıştır. Esrar Dede’nin bir Mevlevî olduğu göz önüne alındığında renklerin onun şiirinde ne kadar önemli olduğu anlaşılır. Bu

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to sugar-free chewing gum sweetened with xylitol and plaque acid neutralisation (ID 485), maintenance of tooth mineralisation (ID 486, 562, 1181), reduction of dental

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to sugar-free chewing gum sweetened with xylitol and plaque acid neutralisation, maintenance of tooth mineralisation, reduction of dental plaque, and defence against pathogens in the middle ear. The scientific substantiation is based on the information provided by the Member States...... in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food that is the subject of the health claims is sugar-free chewing gum sweetened with xylitol. The Panel considers that sugar-free chewing gum sweetened with xylitol...

Kinetics of periodate oxidation of tris -(4,4'-dimethyl-2,2'-bipyridine ...

African Journals Online (AJOL)

dimethyl-2,2'-bipyridine) iron(II) in acid medium was investigated. The complex undergoes extensive protonation in acid medium. Both protonated and the unprotonated species undergo electron transfer reaction with the active periodate species ...

DNA-Binding Studies of Some Potential Antitumor 2,2'-bipyridine Pt(II)/Pd(II) Complexes of piperidinedithiocarbamate. Their Synthesis, Spectroscopy and Cytotoxicity.

Science.gov (United States)

Mansouri-Torshizi, Hassan; Eslami-Moghadam, Mahboube; Divsalar, Adeleh; Saboury, Ali-Akbar

2011-12-01

In this study two platinum(II) and palladium(II) complexes of the type [M(bpy)(pip-dtc)]NO3 (where M=Pt(II) or Pd(II), bpy=2,2'-bipyridine, pip-dtc=piperidinedithiocarbamate) were synthesized by reaction between diaquo-2,2'-bipyridine Pt(II)/Pd(II) nitrate and sodium salt of dithiocarbamate. These cationic water soluble complexes were characterized by elemental analysis, molar conductance, IR, electronic and 1H NMR spectroscopic studies. The cyclic dithiocarbamate was found to coordinate as bidentate fasion with Pt(II) or Pd(II) center. Their biological activities were tested against chronic myelogenous leukemia cell line, K562, at micromolar concentration. The obtained cytotoxic concentration (IC50) values were much lower than cisplatin. The interaction of these complexes with highly polymerized calf thymus DNA (ct-DNA) was extensively studied by means of electronic absorption, fluorescence, circular dichroism and other measurements. The experimental results, thermodynamic and binding parameters, suggested that these complexes cooperatively bind to DNA presumably via intercalation. Moreover, the tendency of the Pt(II) complex to interact with DNA was more than that of Pd(II) complex.

Simultaneous Measurement of Nitrous Acid,Nitric Acid, and Nitrogen Dioxide by Means \

Czech Academy of Sciences Publication Activity Database

Vichi, F.; Mašková, Ludmila; Frattoni, M.; Imperiali, A.; Smolík, Jiří

2016-01-01

Roč. 4, FEB 22 (2016), s. 4 ISSN 2050-7445. [International Conference on Indoor Air Quality in Heritage and Historic Environments /11./. Prague, 13.04.2014-16.04.2014] Institutional support: RVO:67985858 Keywords : diffusive sampling * nitrous acid * nitric acid Subject RIV: CF - Physical ; Theoretical Chemistry

Synthesis of phosphonic acid derivatized bipyridine ligands and their ruthenium complexes.

Science.gov (United States)

Norris, Michael R; Concepcion, Javier J; Glasson, Christopher R K; Fang, Zhen; Lapides, Alexander M; Ashford, Dennis L; Templeton, Joseph L; Meyer, Thomas J

2013-11-04

Water-stable, surface-bound chromophores, catalysts, and assemblies are an essential element in dye-sensitized photoelectrosynthesis cells for the generation of solar fuels by water splitting and CO2 reduction to CO, other oxygenates, or hydrocarbons. Phosphonic acid derivatives provide a basis for stable chemical binding on metal oxide surfaces. We report here the efficient synthesis of 4,4'-bis(diethylphosphonomethyl)-2,2'-bipyridine and 4,4'-bis(diethylphosphonate)-2,2'-bipyridine, as well as the mono-, bis-, and tris-substituted ruthenium complexes, [Ru(bpy)2(Pbpy)](2+), [Ru(bpy)(Pbpy)2](2+), [Ru(Pbpy)3](2+), [Ru(bpy)2(CPbpy)](2+), [Ru(bpy)(CPbpy)2](2+), and [Ru(CPbpy)3](2+) [bpy = 2,2'-bipyridine; Pbpy = 4,4'-bis(phosphonic acid)-2,2'-bipyridine; CPbpy = 4,4'-bis(methylphosphonic acid)-2,2'-bipyridine].

Tahsin Ömer’in “Darb-I Mesellerimiz Hakkında Tahlili Tedkikat” Adlı Atasözleri Seçkisi A Selection of Proverbs from Tahsin Ömer’s “Darb-ı Mesellerimiz Hakkında Tahlili Tedkikat”

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Abdulmuttalip İPEK

2012-09-01

Full Text Available Proverbs and idioms include very different matters ranging from the life styles of the societies they belong to to their frames of mind, from standards of judgements to moral insights due to the fact that they are based on lasting observations, experiences and lives of those societies. As a result, they are stereotyped words which are on the position of the soul of all the culture and value world of the nation they belong to. Proverbs, which make a short-cut and efficient expression possible, also reflect the philosophy of a nation. From this point of view, the realization and preservation of these verbal entities are of vital importance. From the time of Diwanu Lugat et-Turk, the oldest known dictionary of Turkish language, to our day, important dictionaries have been authored to preserve and record these verbal entities. Each of these dictionaries are very important resources as regards with Turkish language and culture. This manuscript will mention about an anthology of proverbs. According to the searches and surveys the anthology has been mentioned only in a few articles until today. The booklet “Darb-ı Mesellerimiz Hakkında Tahlili Tedkikat” was written by Tahsin Ömer and was printed in Evkaf Printing House in Şehzadebaşı, İstanbul between 1337 and 1340. The printed copy of the booklet in the library of the University of Toronto with the recording number of PN 6505 T8 035, is translated into Modern Turkish from Ottoman Turkish with an additional short review. As the result of a revisal of about 5000 proverbs used in our country, nearly 200 proverbs were sorted out according to their meanings and Tahsin Ömer says in the two and a half pages of foreword that this 16 pages booklet was written to make people learn and draw some lessons. The most exciting parts of this booklet are that the writer examines the proverbs in moral aspects and presents a sorting method. Another important part is the presented analytical assasments in the

Kanser hastalarında kemoterapinin C-reaktif protein düzeyine ve yaşam kalitesine olan etkileri

OpenAIRE

Gürler, Mehmet Yavuz

2011-01-01

Giriş ve Amaç: Bir inflamasyon belirteci ve akut faz reaktanı olan C-reaktif protein, karaciğer ve yağ dokusunda üretilen bir beta globulindir. Günümüz pratiğinde pek çok hastalığın tanısını desteklerken bazı hastalıkların ise takibinde ve alevlenmesinde güvenilir bir belirteçtir. Son yıllarda yapılan çalışmalar göstermiş ki kanser hastalarında bakılan serum CRP düzeyi hastalığın yaygınlığı, hastanın yaşam kalitesi, prognozu ve kemoterapiye yanıtı değerlendirmede kullanılabilme...

Recombinant adeno-associated virus mediates a high level of gene transfer but less efficient integration in the K562 human hematopoietic cell line.

Science.gov (United States)

Malik, P; McQuiston, S A; Yu, X J; Pepper, K A; Krall, W J; Podsakoff, G M; Kurtzman, G J; Kohn, D B

1997-03-01

We tested the ability of a recombinant adeno-associated virus (rAAV) vector to express and integrate exogenous DNA into human hematopoietic cells in the absence of selection. We developed an rAAV vector, AAV-tNGFR, carrying a truncated rat nerve growth factor receptor (tNGFR) cDNA as a cell surface reporter under the control of the Moloney murine leukemia virus (MoMuLV) long terminal repeat. An analogous MoMuLV-based retroviral vector (L-tNGFR) was used in parallel, and gene transfer and expression in human hematopoietic cells were assessed by flow cytometry and DNA analyses. Following gene transfer into K562 cells with AAV-tNGFR at a multiplicity of infection (MOI) of 13 infectious units (IU), 26 to 38% of cells expressed tNGFR on the surface early after transduction, but the proportion of tNGFR expressing cells steadily declined to 3.0 to 3.5% over 1 month of culture. At an MOI of 130 IU, nearly all cells expressed tNGFR immediately posttransduction, but the proportion of cells expressing tNGFR declined to 62% over 2 months of culture. The decline in the proportion of AAV-tNGFR-expressing cells was associated with ongoing losses of vector genomes. In contrast, K562 cells transduced with the retroviral vector L-tNGFR expressed tNGFR in a constant fraction. Integration analyses on clones showed that integration occurred at different sites. Integration frequencies were estimated at about 49% at an MOI of 130 and 2% at an MOI of 1.3. Transduction of primary human CD34+ progenitor cells by AAV-tNGFR was less efficient than with K562 cells and showed a declining percentage of cells expressing tNGFR over 2 weeks of culture. Thus, purified rAAV caused very high gene transfer and expression in human hematopoietic cells early after transduction, which steadily declined during cell passage in the absence of selection. Although the efficiency of integration was low, overall integration was markedly improved at a high MOI. While prolonged episomal persistence may be adequate

Screening and identification of lactic acid bacteria strains with high acid-producing from traditional fermented yak yogurt

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Chen Xiaoyong

2017-01-01

Full Text Available A total of 57 strains of lactic acid bacteria (LAB were isolated and purified from traditional fermented Yak Yogurt in Hongyuan-Sichuan and Yangbajing-Tibet. The strains with high acid-produced were screened by soluble calcium circle and titratable acidity determination. The five strains, 7-1, 22-1, 28-1, 34-1 and 62-1, possessed the high acid-producing and the value of titratable acidity is 196.2, 191.1, 192.2, 194.8 and 200.2 T respectively. Based on 16S rDNA sequence analysis, 22-1 was identified as Lactococcus lactis subsp. lactis, 28-1 as Lactobacillus casei, 34-1 as Lactobacillus fermentium, 7-1 and 62-1 as Enterococcus durans. This study could provide the evidence for researching fermentation strains to improve yogurt quality.

Potential for food-drug interactions by dietary phenolic acids on human organic anion transporters 1 (SLC22A6), 3 (SLC22A8), and 4 (SLC22A11).

Science.gov (United States)

Wang, Li; Sweet, Douglas H

2012-10-15

Phenolic acids exert beneficial health effects such as anti-oxidant, anti-carcinogenic, and anti-inflammatory activities and show systemic exposure after consumption of common fruits, vegetables, and beverages. However, knowledge regarding which components convey therapeutic benefits and the mechanism(s) by which they cross cell membranes is extremely limited. Therefore, we determined the inhibitory effects of nine food-derived phenolic acids, p-coumaric acid, ferulic acid, gallic acid, gentisic acid, 4-hydroxybenzoic acid, protocatechuic acid, sinapinic acid, syringic acid, and vanillic acid, on human organic anion transporter 1 (hOAT1), hOAT3, and hOAT4. In the present study, inhibition of OAT-mediated transport of prototypical substrates (1 μM) by phenolic acids (100 μM) was examined in stably expressing cell lines. All compounds significantly inhibited hOAT3 transport, while just ferulic, gallic, protocatechuic, sinapinic, and vanillic acid significantly blocked hOAT1 activity. Only sinapinic acid inhibited hOAT4 (~35%). For compounds exhibiting inhibition > ~60%, known clinical plasma concentration levels and plasma protein binding in humans were examined to select compounds to evaluate further with dose-response curves (IC(50) values) and drug-drug interaction (DDI) index determinations. IC(50) values ranged from 1.24 to 18.08 μM for hOAT1 and from 7.35 to 87.36 μM for hOAT3. Maximum DDI indices for gallic and gentisic acid (≫0.1) indicated a very strong potential for DDIs on hOAT1 and/or hOAT3. This study indicates that gallic acid from foods or supplements, or gentisic acid from salicylate-based drug metabolism, may significantly alter the pharmacokinetics (efficacy and toxicity) of concomitant therapeutics that are hOAT1 and/or hOAT3 substrates. Copyright © 2012 Elsevier Inc. All rights reserved.

Seyyid Vehbi'nin Divanında Yer Almayan Bir Kasidesi The Kaside That Doesn’t Take Place In Divan Of Seyyid Vehbi

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Haluk GÖKALP

2013-03-01

Full Text Available Perodicals include kinds of poems. In addition they comprise artistic and cultural accumulation when they were written. Pastiche collections give knowledge abaut pastiche tradition and interactions between poets. Poem notebooks which can collect diffrent subjects contribute to history of literatüre. So, pastiche collections or other collections are one of the main source of classical poetry. Contributions of periodicals to classical school of poetry investigating, may be collected with three groups. Firsh of all, periodicals reflect the plcasure of poem when they were written and poems which were approued can be determined. Second one, they contribute to recognition of poets who weren’t mentioned in history of literature. Third one, investigetors can benefit from periodicals for poems which weren’t mentioned in poet’s divan which include their poems. By this way, investigators can collect all poems of poet. İn this study, we evalvated the contributions of periodicals to classical poetry investigators. And than we are going to publish Seyyid Vehbi’s kaside in “Sıhhatname ve Sur-ı Hıtana Müteallik Kasaid” which was found from Topkapı Palace Library. With this study, Seyyid Vehbi’s poem which weren’t mentioned in other 31 copy is going to be presented to investigator’s advantage. Şiir mecmuaları çeşitli şiirleri derlemekle birlikte yazıldığı devrin sanat ve kültür birikimini de bir araya getirirler. Nazire mecmuaları nazire geleneğini ve şairler arası etkileşimi örnekleriyle gözler önüne sererken, farklı konular etrafında toplanan ya da -tamamen okuyucunun zevkine göre gelişigüzel toplanan- şiir defterleri edebiyat tarihini bir bütünlük içinde görmemize olumlu katkılar sağlar. Bu nedenle nazire mecmuaları ya da diğer şiir mecmuaları, divan şiiri araştırmalarının başlıca kaynaklarındandır. Genel olarak baktığımızda mecmuaların divan edebiyatı araştırmalarına katk

Active Hydrophilic Components of the Medicinal Herb Salvia miltiorrhiza (Danshen Potently Inhibit Organic Anion Transporters 1 (Slc22a6 and 3 (Slc22a8

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Li Wang

2012-01-01

Full Text Available Many active components of herbal products are small organic anions, and organic anion transporters were previously demonstrated to be a potential site of drug-drug interactions. In this study, we assessed the inhibitory effects of six hydrophilic components of the herbal medicine Danshen, lithospermic acid, protocatechuic acid, rosmarinic acid, salvianolic acid A, salvianolic acid B, and tanshinol, on the function of the murine organic anion transporters, mOat1 and mOat3. All of Danshen components significantly inhibited mOat1- and mOat3-mediated substrate uptake (<0.001 with lithospermic acid (LSA, protocatechuic acid, rosmarinic acid (RMA, and salvianolic acid A (SAA producing virtually complete inhibition under test conditions. Kinetic analysis demonstrated that LSA, RMA, and SAA were competitive inhibitors. As such, values were estimated as 14.9±4.9 μM for LSA, 5.5±2.2 μM for RMA, and 4.9±2.2 μM for SAA on mOat1-mediated transport, and as 31.1±7.0 μM for LSA, 4.3±0.2 μM for RMA, and 21.3±7.7 μM for SAA on mOat3-mediated transport. These data suggest that herb-drug interactions may occur in vivo on the human orthologs of these transporters in situations of polypharmacy involving Danshen and clinical therapeutics known to be organic anion transporter substrates.

The Palmitoylation State of PMP22 Modulates Epithelial Cell Morphology and Migration

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Susie J. Zoltewicz

2012-11-01

Full Text Available PMP22 (peripheral myelin protein 22, also known as GAS 3 (growth-arrest-specific protein 3, is a disease-linked tetraspan glycoprotein of peripheral nerve myelin and constituent of intercellular junctions in epithelia. To date, our knowledge of the post-translational modification of PMP22 is limited. Using the CSS-Palm 2.0 software we predicted that C85 (cysteine 85, a highly conserved amino acid located between the second and third transmembrane domains, is a potential site for palmitoylation. To test this, we mutated C85S (C85 to serine and established stable cells lines expressing the WT (wild-type or the C85S-PMP22. In Schwann and MDCK (Madin–Darby canine kidney cells mutating C85 blocked the palmitoylation of PMP22, which we monitored using 17-ODYA (17-octadecynoic acid. While palmitoylation was not necessary for processing the newly synthesized PMP22 through the secretory pathway, overexpression of C85S-PMP22 led to pronounced cell spreading and uneven monolayer thinning. To further investigate the functional significance of palmitoylated PMP22, we evaluated MDCK cell migration in a wound-healing assay. While WT-PMP22 expressing cells were resistant to migration, C85S cells displayed lamellipodial protrusions and migrated at a similar rate to vector control. These findings indicate that palmitoylation of PMP22 at C85 is critical for the role of the protein in modulating epithelial cell shape and motility.

Pnömatik Ekim Makinalarında Farklı Tip Dağıtma Başlıkları, Hava Hızı ve Gübreleme Normunun Akış Düzgünlüğüne Etkisi / The Effects of Distributor Head Types, Air Velocity and Fertilizing Rate on Flow Evenness in Pneumatic Seed Drills

OpenAIRE

Güler, İbrahim Ethem; Uygan, Fatih

2011-01-01

ÖZET : Bu araştırma pnömatik tahıl ekim makinalarında kullanılan farklı tip dağıtma başlıklarında, hava hızının ve gübrelemenormunun akış düzgünlüğüne etkisini belirlemek için yapılmıştır. Araştırmada üç farklı tip dağıtma başlığı (T, Huni ve Y), üç farklıhava hızında (26, 31, 36 m/s) denenmiştir. Denemelerde Diamonyum fosfat (DAP) ve Triple süper fosfat (TSP) gübrelerikullanılmıştır. Gübreleme normları 12, 16 ve...

Stereoselective chemoenzymatic synthesis of the four stereoisomers of l-2-(2-carboxycyclobutyl)glycine and pharmacological characterization at human excitatory amino acid transporter subtypes 1, 2, and 3

DEFF Research Database (Denmark)

Faure, Sophie; Jensen, Anders A.; Maurat, Vincent

2006-01-01

The four stereoisomers of l-2-(2-carboxycyclobutyl)glycine, l-CBG-I, l-CBG-II, l-CBG-III, and l-CBG-IV, were synthesized in good yield and high enantiomeric excess, from the corresponding cis and trans-2-oxalylcyclobutanecarboxylic acids 5 and 6 using the enzymes aspartate aminotransferase (AAT......) and branched chain aminotransferase (BCAT) from Escherichia coli. The four stereoisomeric compounds were evaluated as potential ligands for the human excitatory amino acid transporters, subtypes 1, 2, and 3 (EAAT1, EAAT2, and EAAT3) in the FLIPR membrane potential assay. While the one trans-stereoisomer, l...

Interfacial behavior of N-nitrosodiethylamine/bovine serum albumin complexes at the air-water and the chloroform-water interfaces by axisymmetric drop tensiometry.

Science.gov (United States)

Juárez, J; Galaz, J G; Machi, L; Burboa, M; Gutiérrez-Millán, L E; Goycoolea, F M; Valdez, M A

2007-03-15

Interfacial properties of N-nitrosodiethylamine/bovine serum albumin (NDA/BSA) complexes were investigated at the air-water interface. The interfacial behavior at the chloroform-water interface of the interaction product of phospholipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), dissolved in the chloroform phase, and NDA/BSA complex, in the aqueous phase, were also analyzed by using a drop tensiometer. The secondary structure changes of BSA with different NDA concentrations were monitored by circular dichroism spectroscopy at different pH and the NDA/BSA interaction was probed by fluorescence spectroscopy. Different NDA/BSA mixtures were prepared from 0, 7.5 x 10(-5), 2.2 x 10(-4), 3.7 x 10(-4), 5 x 10(-4), 1.6 x 10(-3), and 3.1 x 10(-3) M NDA solutions in order to afford 0, 300/1, 900/1, 1 500/1, 2 000/1, 6 000/1, and 12 500/1 NDA/BSA molar ratios, respectively, in the aqueous solutions. Increments of BSA alpha-helix contents were obtained up to the 2 000/1 NDA/BSA molar ratio, but at ratios beyond this value, the alpha-helix content practically disappeared. These BSA structure changes produced an increment of the surface pressure at the air-water interface, as the alpha-helix content increased with the concentration of NDA. On the contrary, when alpha-helix content decreased, the surface pressure also appeared lower than the one obtained with pure BSA solutions. The interaction of DPPC with NDA/BSA molecules at the chloroform-water interface produced also a small, but measurable, pressure increment with the addition of NDA molecules. Dynamic light scattering measurements of the molecular sizes of NDA/BSA complex at pH 4.6, 7.1, and 8.4 indicated that the size of extended BSA molecules at pH 4.6 increased in a greater proportion with the increment in NDA concentration than at the other studied pH values. Diffusion coefficients calculated from dynamic surface tension values, using a short-term solution of the general adsorption model of Ward and Tordai

Öğütülmüş Ayçiçek Tohumlarında Enzim Muamelesiyle Yağ Üretiminin Arttırılması

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Mariyana Perifanova-Nemska

2015-02-01

Full Text Available Öğütülmüş ayçiçek tohumları daha fazla yağ elde etmek amacı ile sellülaz, asit ve baz karakterli proteinaz ve pektinaz enzimleri ile muamele edilmiştir. İncelenen preparatlar arasında sellülaz (selobranin enzimi uygulanan tohumlar en yüksek yağ verimini göstermişlerdir.

Net alkalinity and net acidity 1: Theoretical considerations

International Nuclear Information System (INIS)

Kirby, Carl S.; Cravotta, Charles A.

2005-01-01

Net acidity and net alkalinity are widely used, poorly defined, and commonly misunderstood parameters for the characterization of mine drainage. The authors explain theoretical expressions of 3 types of alkalinity (caustic, phenolphthalein, and total) and acidity (mineral, CO 2 , and total). Except for rarely-invoked negative alkalinity, theoretically defined total alkalinity is closely analogous to measured alkalinity and presents few practical interpretation problems. Theoretically defined 'CO 2 -acidity' is closely related to most standard titration methods with an endpoint pH of 8.3 used for determining acidity in mine drainage, but it is unfortunately named because CO 2 is intentionally driven off during titration of mine-drainage samples. Using the proton condition/mass-action approach and employing graphs to illustrate speciation with changes in pH, the authors explore the concept of principal components and how to assign acidity contributions to aqueous species commonly present in mine drainage. Acidity is defined in mine drainage based on aqueous speciation at the sample pH and on the capacity of these species to undergo hydrolysis to pH 8.3. Application of this definition shows that the computed acidity in mgL -1 as CaCO 3 (based on pH and analytical concentrations of dissolved Fe II , Fe III , Mn, and Al in mgL -1 ):acidity calculated =50{1000(10 -pH )+[2(Fe II )+3(Fe III )]/56+2(Mn) /55+3(Al)/27}underestimates contributions from HSO 4 - and H + , but overestimates the acidity due to Fe 3+ and Al 3+ . However, these errors tend to approximately cancel each other. It is demonstrated that 'net alkalinity' is a valid mathematical construction based on theoretical definitions of alkalinity and acidity. Further, it is shown that, for most mine-drainage solutions, a useful net alkalinity value can be derived from: (1) alkalinity and acidity values based on aqueous speciation (2) measured alkalinity minus calculated acidity, or (3) taking the negative of the

Identification of a functional nuclear localization signal within the human USP22 protein

International Nuclear Information System (INIS)

Xiong, Jianjun; Wang, Yaqin; Gong, Zhen; Liu, Jianyun; Li, Weidong

2014-01-01

Highlights: • USP22 was accumulated in nucleus. • We identified of a functional USP22 NLS. • The KRRK amino acid residues are indispensable in NLS. • The KRRK motif is conserved in USP22 homologues. - Abstract: Ubiquitin-specific processing enzyme 22 (USP22), a member of the deubiquitinase family, is over-expressed in most human cancers and has been implicated in tumorigenesis. Because it is an enzymatic subunit of the human SAGA transcriptional cofactor, USP22 deubiquitylates histone H2A and H2B in the nucleus, thus participating in gene regulation and cell-cycle progression. However, the mechanisms regulating its nuclear translocation have not yet been elucidated. It was here demonstrated that USP22 is imported into the nucleus through a mechanism mediated by nuclear localization signal (NLS). The bipartite NLS sequence KRELELLKHNPKRRKIT (aa152–168), was identified as the functional NLS for its nuclear localization. Furthermore, a short cluster of basic amino acid residues KRRK within this bipartite NLS plays the primary role in nuclear localization and is evolutionarily conserved in USP22 homologues. In the present study, a functional NLS and the minimal sequences required for the active targeting of USP22 to the nucleus were identified. These findings may provide a molecular basis for the mechanism underlying USP22 nuclear trafficking and function

Identification of a functional nuclear localization signal within the human USP22 protein

Energy Technology Data Exchange (ETDEWEB)

Xiong, Jianjun [Key Laboratory of Jiangxi Province for the Systems Bio-Medicine, Jiujiang, Jiangxi Province 332000 (China); College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi Province 332000 (China); Wang, Yaqin [Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei Province 430060 (China); Gong, Zhen [Key Laboratory of Jiangxi Province for the Systems Bio-Medicine, Jiujiang, Jiangxi Province 332000 (China); Liu, Jianyun [College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi Province 332000 (China); Li, Weidong, E-mail: lwd626518@163.com [College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi Province 332000 (China)

2014-06-20

Highlights: • USP22 was accumulated in nucleus. • We identified of a functional USP22 NLS. • The KRRK amino acid residues are indispensable in NLS. • The KRRK motif is conserved in USP22 homologues. - Abstract: Ubiquitin-specific processing enzyme 22 (USP22), a member of the deubiquitinase family, is over-expressed in most human cancers and has been implicated in tumorigenesis. Because it is an enzymatic subunit of the human SAGA transcriptional cofactor, USP22 deubiquitylates histone H2A and H2B in the nucleus, thus participating in gene regulation and cell-cycle progression. However, the mechanisms regulating its nuclear translocation have not yet been elucidated. It was here demonstrated that USP22 is imported into the nucleus through a mechanism mediated by nuclear localization signal (NLS). The bipartite NLS sequence KRELELLKHNPKRRKIT (aa152–168), was identified as the functional NLS for its nuclear localization. Furthermore, a short cluster of basic amino acid residues KRRK within this bipartite NLS plays the primary role in nuclear localization and is evolutionarily conserved in USP22 homologues. In the present study, a functional NLS and the minimal sequences required for the active targeting of USP22 to the nucleus were identified. These findings may provide a molecular basis for the mechanism underlying USP22 nuclear trafficking and function.

Design, synthesis and docking studies of novel dipeptidyl boronic acid proteasome inhibitors constructed from αα- and αβ-amino acids.

Science.gov (United States)

Shi, Jingmiao; Lei, Meng; Wu, Wenkui; Feng, Huayun; Wang, Jia; Chen, Shanshan; Zhu, Yongqiang; Hu, Shihe; Liu, Zhaogang; Jiang, Cheng

2016-04-15

A series of novel dipeptidyl boronic acid proteasome inhibitors constructed from αα- and αβ-amino acids were designed and synthesized. Their structures were elucidated by (1)H NMR, (13)C NMR, LC-MS and HRMS. These compounds were evaluated for their β5 subunit inhibitory activities of human proteasome. The results showed that dipeptidyl boronic acid inhibitors composed of αα-amino acids were as active as bortezomib. Interestingly, the activities of those derived from αβ-amino acids lost completely. Of all the inhibitors, compound 22 (IC50=4.82 nM) was the most potent for the inhibition of proteasome activity. Compound 22 was also the most active against three MM cell lines with IC50 values less than 5 nM in inhibiting cell growth assays. Molecular docking studies displayed that 22 fitted very well in the β5 subunit active pocket of proteasome. Copyright © 2016. Published by Elsevier Ltd.

1911-1918 Yılları Arasında Türk Yurdu Dergisine Konu Olan Bazı Tartışma ve Polemikler / Some Polemics and Discussions which were Subjected In Turk Yurdu Magazine Between 1911-1918

Directory of Open Access Journals (Sweden)

Ümmet Erkan

2016-01-01

Bazı Tartışma ve Polemikler  Öz Türk Yurdu Dergisi 1911 yılında yayın hayatına başlamış, Türk milliyetçiliinin en uzun soluklu yayın organıdır. Rusya Türklerinin öncülük ettiği dergide Türklüğün her alanda yükselmesi amaçlanmıştır. Bu makalede Türk Yurdu dergisinin 1911-1918 yılları arasındaki sayılarından yola çıkılarak dergideki bazı kalem tartışmaları, mektuplar ve onlara verilen cevaplar incelenecektir. Bu kalem tartışmalarında Türkçenin sadeleştirilmesinden ne anlaşılması gerektiği ve sınırları, eğitimde ideal eksikliği, eğitim dilinin Türkçe olması ve azınlık okullarının Osmanlı sosyal bünyesine verdiği zararlar, Tanzimatçı reform programının başarılı olup olmadığı ve nedenleri, milli kimliğin dini aidiyete zarar verip vermeyeceği konuları ele alınacaktır. Türk Yurdu dergisi yazarları ile dönemin diğer önemli entelektüellerinin çeşitli gazete ve dergilerde dâhil olduğu bu kalem tartışmaları zengin bir muhteva kazanmıştır. Bu tartışmalar dönemin entelektüel fikir hareketleri ve siyasi akımlar hakkında önemli bilgiler içermektedir. Bu araştırma, yapılan tartışma ve polemiklerin hangi konular etrafında yoğunlaştığını ve Türk Yurdu dergisinin bu tartışma ve polemiklerde ne tür bir yöntem izlediğini ortaya koymaktadır.

Emanuel Sendromu

OpenAIRE

M, Kazandı; V, Turan; B, Zeybek; A, rgon

2010-01-01

35 yaşında daha önceden 1 aylık spontan abortusu bulunan ve miad sezaryen doğum ile 3300gr sağlıklı çocuğu olan hastada şimdiki gebeliğinde yapılan 2. düzey taramasında ; nukal foldda kalınlaşma, diafragma hernisi, komplet endokardial yastık defekti ve trunkus arteriosis saptanması üzerine amniosentez yapıldı ve gebelik sonlandırılmasına gidildi. Misoprostol 200mg 6x1 uygulandı. Yapılan genetik incelemede 47, .. , +der(22) t(11,22) (q25;q13) saptandı. Aileye yapılan kromozom analizinde an...

Cellular uptake, nuclear localization and cytotoxicity of 125I-labelled DNA minor groove binding ligands in K562, human erythroleukaemia cells

International Nuclear Information System (INIS)

Karagiannis, T.C.; Lobachevsky, P.N.; Martin, R.F.

2000-01-01

Full text: Iodine-125 decays by orbital electron capture and internal conversion resulting in the emission of numerous Auger electrons which produce a highly localised radiochemical damage in the immediate vicinity of the site of decay. Given the requirement to deliver 125 I to the nuclear DNA, a minor groove binding bibenzimidazole, 125 I-iodoHoechst 33258 was investigated. It has been noted that this analogue may be prone to de-iodination in vitro and in vivo, given the presence of an orthoiodophenol moiety which is analogous to that in thyroxins. Therefore, an 125 I -iodoHoechst analogue without the hydroxyl group was also studied. The 125 I -iodoHoechst 33258 analogue was prepared by direct iodination of Hoechst 33258 and 125 I iodoHoechst was prepared by demetallation of a trimethylstannyl precursor. DNA binding studies indicated that both iodo-analogues bind to calf thymus DNA, K D = 89 ± 30nM, n = 0.018 bp - 1 for iodoHoechst 33258 and K D = 121 ± 31nM, n = 0.024 bp -1 for iodoHoechst. Similarly, nuclear localization following incubation with 5μM of either ligand at 37 deg C was observed in K562 cells by fluorescence microscopy. Flow cytometry was used to investigate the kinetics of drug uptake and efflux in K562 cells. The results indicated that when 10 6 cells were incubated with 5μM ligand at 37 deg C, the uptake reached a plateau at approximately 43 minutes for iodoHoechst 33258 and approximately 52 minutes for iodoHoechst. Ligand efflux results indicated two-phase kinetics. The initial phase which involves 50-60% of drug was characterised by a half-life time (t 1/2 ) of 55.4 minutes for efflux of iodoHoechst 33258 and a t 1/2 of 10.3 minutes for efflux of iodoHoechst, at 37 deg C. Furthermore, the results suggested that the DNA binding sites in a 10 6 cell/ml suspension were saturated by incubation with 3μM iodoHoechst 33258 and 5μM iodoHoechst. In the initial cytotoxicity experiments using 125 I-iodoHoechst 33258, K562 cells were incubated for 1

Ciceronov preklad Platónovej Politeie (562c-563e v spise De re publica (I, 66 – 67 (Cicero’s Translation of the Plato’s Dialogue Republic (562c-563e in the Treatise On the Commonwealth (I, 66 – 67

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Peter Fraňo

2015-09-01

Full Text Available The paper focuses on comparasion of the Greek original from Plato’s dialogue Republic (562c-563e with the Latin translation, which Cicero cites in the treatise On the Commonwealth (I, 66 – 67. Cicero in this work changes the sense of the Greek text by the various formal and meaning techniques. Author removes the dialogical constitution of the Plato’s passages and omits from the Latin text expressly Greek terms. Further, Cicero changes Plato’s sarcastic tone and prefers more formal and critique style. With using these techniques he discredits of Greek model of democratic’s freedom (a person has license „to do what he wants“ and evolves more acceptable Roman democratic’s freedom (capable of possessing rights of one’s own.

Interaction of (n-3) and (n-6) fatty acids in desaturation and chain elongation of essential fatty acids in cultured glioma cells

International Nuclear Information System (INIS)

Cook, H.W.; Spence, M.W.

1987-01-01

Recent research in various biological systems has revived interest in interactions between the (n-6) and (n-3) essential fatty acids. We have utilized cultured glioma cells to show that linolenic acid, 18:3(n-3), is rapidly desaturated and chain elongated; 20:5(n-3) is the major product and accumulates almost exclusively in phospholipids. We examined effects of various (n-6), (n-3), (n-9) and (n-7) fatty acids at 40 microM concentration on desaturation and chain elongation processes using [1- 14 C]18:3(n-3) as substrate. In general, monoenoic fatty acids were without effect. The (n-6) fatty acids (18:2, 18:3, 20:3, 20:4 and 22:4) had little effect on total product formed. There was a shift of labeled product to triacylglycerol, and in phospholipids, slightly enhanced conversion of 20:5 to 22:5 was evident. In contrast, 22:6(n-3) was inhibitory, whereas 20:3(n-3) and 20:5(n-3) had much less effect. At concentrations less than 75 microM, all acids were inhibitory. Most products were esterified to phosphatidylcholine, but phosphatidylethanolamine also contained a major portion of 20:5 and 22:5. We provide a condensed overview of how the (n-6) and (n-3) fatty acids interact to modify relative rates of desaturation and chain elongation, depending on the essential fatty acid precursor. Thus, the balance between these dietary acids can markedly influence enzymes providing crucial membrane components and substrates for biologically active oxygenated derivatives

A Novel Soybean Dirigent Gene GmDIR22 Contributes to Promotion of Lignan Biosynthesis and Enhances Resistance to Phytophthora sojae

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Ninghui Li

2017-07-01

Full Text Available Phytophthora root and stem rot caused by the oomycete pathogen Phytophthora sojae is a destructive disease of soybean worldwide. Plant dirigent proteins (DIR are proposed to have roles in biosynthesis of either lignan or lignin-like molecules, and are important for defense responses, secondary metabolism, and pathogen resistance. In the present work, a novel DIR gene expressed sequence tag is identified as up-regulated in the highly resistant soybean cultivar ‘Suinong 10’ inoculated with P. sojae. The full length cDNA is isolated using rapid amplification of cDNA ends, and designated GmDIR22 (GenBank accession no. HQ_993047. The full length GmDIR22 is 789 bp and contains a 567 bp open reading frame encoding a polypeptide of 188 amino acids. The sequence analysis indicated that GmDIR22 contains a conserved dirigent domain at amino acid residues 43–187. The quantitative real-time reverse transcription PCR demonstrated that soybean GmDIR22 mRNA is expressed most highly in stems, followed by roots and leaves. The treatments with stresses demonstrated that GmDIR22 is significantly induced by P. sojae and gibberellic acid (GA3, and also responds to salicylic acid, methyl jasmonic acid, and abscisic acid. The GmDIR22 is targeted to the cytomembrane when transiently expressed in Arabidopsis protoplasts. Moreover, The GmDIR22 recombinant protein purified from Escherichia coli could effectively direct E-coniferyl alcohol coupling into lignan (+-pinoresinol. Accordingly, the overexpression of GmDIR22 in transgenic soybean increased total lignan accumulation. Moreover, the lignan extracts from GmDIR22 transgenic plants effectively inhibits P. sojae hyphal growth. Furthermore, the transgenic overexpression of GmDIR22 in the susceptible soybean cultivar ‘Dongnong 50’ enhances its resistance to P. sojae. Collectively, these data suggested that the primary role of GmDIR22 is probably involved in the regulation of lignan biosynthesis, and which

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and gamma-linolenic acid (GLA) and contribution to normal cognitive function (ID 532) and maintenance

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and gamma-linolenic acid (GLA) and contribution to normal cognitive function and maintenance of normal bone. The scientific substantiation is based on the information provided by the Member States in the consolidated list...... and fish oil”. From the references provided, the Panel assumes that the food constituents that are the subject of the claims are the n-6 fatty acid gamma-linolenic acid (GLA) in evening primrose oil and the n-3 long-chain polyunsaturated fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA...... of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituents that are the subjects of the health claims are “omega-3 and omega-6 fatty acids (GLA)”, “gamma-linolenic acid + eicosapentaenoic acid (GLA+EPA)”, and “evening primrose oil...

A nanocomplex of Cu(II) with theophylline drug; synthesis, characterization, and anticancer activity against K562 cell line

Science.gov (United States)

Sahlabadi, Maryam; Daryanavard, Marzieh; Hadadzadeh, Hassan; Amirghofran, Zahra

2018-03-01

A new mononuclear of copper (II), [Cu(theophylline)2(H2O)3]·2H2O, has been synthesized by reaction of theophylline (1,3-dimethyl-7H-purine-2,6-dione) with copper (II) nitrate in water. Further, its nanocomplex has been prepared through the three different methods including sonication, grinding, and a combination thereof, sonication-grinding. The prepared nanocomplex was characterized using different techniques including FT-IR, UV-Vis, X-ray diffraction (XRD) analysis, and field-emission scanning electron microscopy (FE-SEM). Moreover, the anticancer activity of the precursor complex, nanocomplex, free theophylline ligand, and the starting copper salt (Cu(NO3)2·3H2O) was investigated against the K562 cell line. The results show that the nanocomplex is an effective nano metal-based anticancer agent with IC50 = 11.7 μM.

In situ object counting system (ISOCSi3TM) technique: A cost-effective tool for NDA verification in IAEA Safeguards

International Nuclear Information System (INIS)

Nizhnik, V.; Belian, A.; Shephard, A.; Lebrun, A.

2011-01-01

Nuclear material measurements using the ISOCS technique are playing an increasing role in IAEA verification activities. The ISOCS capabilities include: a high sensitivity to the presence of U and Pu; the ability to detect very small amounts of material; and the ability to measure items of different shapes and sizes. In addition, the numerical absolute efficiency calibration of a germanium detector used in the technique does not require any calibration standards or reference materials. The ISOCS modelling software performs an absolute efficiency calibration for items with various container shapes, container wall materials, material compositions, material fill-heights, U/Pu weight fractions and even heterogeneously distributed emitting materials. In a number of cases, some key parameters, such as the matrix density and U/Pu weight fraction, can be determined in addition to the emitting material mass and isotopic composition. These capabilities provide a verification solution suitable for a majority of cases where quantitative and isotopic analysis should be performed. Taking into account these advantages, the technique becomes a cost-effective solution for nuclear material non-destructive assay (NDA) verification. At present, the IAEA uses the ISOCS for a wide range of applications including the quantitative analysis of U scrap materials, U/Pu contaminated solid wastes, U fuel elements, U hold-up materials. Additionally, the ISOCS is also applied to some specific verification cases such as the measurement of PuBe neutron sources and the quantification of fission products in solid wastes. In reprocessing facilities with U/Pu waste compaction or facilities with item re-batching, the continuity-of-knowledge can be assured by applying either video surveillance systems together with seals (requiring attaching/detaching and verification activities for each seal) or verification of operator declarations using quantitative measurements for items selected on a random basis

Net alkalinity and net acidity 1: Theoretical considerations

Science.gov (United States)

Kirby, C.S.; Cravotta, C.A.

2005-01-01

Net acidity and net alkalinity are widely used, poorly defined, and commonly misunderstood parameters for the characterization of mine drainage. The authors explain theoretical expressions of 3 types of alkalinity (caustic, phenolphthalein, and total) and acidity (mineral, CO2, and total). Except for rarely-invoked negative alkalinity, theoretically defined total alkalinity is closely analogous to measured alkalinity and presents few practical interpretation problems. Theoretically defined "CO 2-acidity" is closely related to most standard titration methods with an endpoint pH of 8.3 used for determining acidity in mine drainage, but it is unfortunately named because CO2 is intentionally driven off during titration of mine-drainage samples. Using the proton condition/mass- action approach and employing graphs to illustrate speciation with changes in pH, the authors explore the concept of principal components and how to assign acidity contributions to aqueous species commonly present in mine drainage. Acidity is defined in mine drainage based on aqueous speciation at the sample pH and on the capacity of these species to undergo hydrolysis to pH 8.3. Application of this definition shows that the computed acidity in mg L -1 as CaCO3 (based on pH and analytical concentrations of dissolved FeII, FeIII, Mn, and Al in mg L -1):aciditycalculated=50{1000(10-pH)+[2(FeII)+3(FeIII)]/56+2(Mn)/ 55+3(Al)/27}underestimates contributions from HSO4- and H+, but overestimates the acidity due to Fe3+ and Al3+. However, these errors tend to approximately cancel each other. It is demonstrated that "net alkalinity" is a valid mathematical construction based on theoretical definitions of alkalinity and acidity. Further, it is shown that, for most mine-drainage solutions, a useful net alkalinity value can be derived from: (1) alkalinity and acidity values based on aqueous speciation, (2) measured alkalinity minus calculated acidity, or (3) taking the negative of the value obtained in a

Kefir Kültürünün Beyaz Peynir Yapımında Kullanılması Üzerine bir Araştırma

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Hasan Yaygın

2015-02-01

Full Text Available Beyaz peynir yapımında kullanılacak saf kültürde yer alması gereken mikroorganizmaları saptamak amacı ile bazı çalışmalar yapılmıştır. Araştırıcıların çoğunluğu saf kültürde S. lactis, S. cremoris ve L. casei’ nin bulunması gerektiğini belirtmişlerdir. Bu çalışmada beyaz peynir yapımında saf kültür olarak kefir kültürü kullanılmıştır. Kefir kültüründe söz konusu bakterilerle beraber bazı maya ve bakteriler de bulunmaktadır. Araştırma sonuçları kefir kültürü katılan sütten yapılan beyaz peynirde asitlik gelişiminin istenilen düzeyde olduğu; tat ve aroma ile yapının tüketiciler tarafından beğenildiğini ortaya çıkarmıştır.

Kıl Keçi ve Saanen x Kıl Keçi Melezlerinin (F1, G1, Üretici Şartlarında Döl Verim Özellikleri Bakımından Karşılaştırılması

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Hilal Tozlu Çelik

2014-11-01

Full Text Available Bu araştırma, Amasya ili Sarılar köyünde (40°54'23"N, 35°08'dk5"E özel bir işletmede 2011-2012 yılları arasında yetiştirilen Saanen x Kıl keçi melezleri (F1, G1 ve Kıl keçilerinde döl verimi özelliklerini tespit etmek ve bu özellikler üzerine genotip ve yıl faktörlerinin etkisini ortaya koymak amacıyla yapılmıştır. Araştırmada döl verim özelliklerinden 2011 yılı için sadece ikiz doğuran keçi oranı bakımından genotipler arasında istatistiki farklılık belirlenmiştir. İkiz doğuran keçi oranı Kıl keçilerle, F1 melezlerinde benzer bulunmuş ve G1 melezlerinde ise her iki genotipten farklı bulunmuştur. 2012 yılında genotipler arasında kısır kalan keçi, ikiz doğuran keçi ve ölü doğum yapan keçi oranı bakımından istatistiki farklılıklar olduğu tespit edilmiştir. Kısırlık oranının, Saanen x Kıl keçi F1, G1 melezlerinde Kıl keçilerinden daha yüksek olduğu belirlenmiştir. İkiz doğuran keçi oranı F1 genotipinde yüksek, G1 genotipinde ve Kıl keçilerinde benzer bulunmuştur.

Optical Absorption, Stability and Structure of NpO2+ Complexes with Dicarboxylic Acids

International Nuclear Information System (INIS)

Guoxin Tian; Linfeng Rao

2006-01-01

Complexation of NpO2+ with oxalic acid (OX),2,2'-oxydiacetic acid (ODA), 2,2'-iminodiacetic acid (IDA) and 2,2'-thiodiacetic acid (TDA), has been studied using spectrophotometry in1 M NaClO4. Both the position and the intensity of the absorption band of NpO2+ at 980 nm are affected by the formation of NpO2+/dicarboxylate complexes, providing useful information on the complexation strength, the coordination mode and the structure of the complexes

Electrogenerated chemiluminescence quenching of Ru(bpy){sub 3} {sup 2+} (bpy=2,2 Prime -bipyridine) in the presence of acetaminophen, salicylic acid and their metabolites

Energy Technology Data Exchange (ETDEWEB)

Haslag, Catherine S. [Department of Chemistry, Missouri State University, Springfield, Missouri 65897 (United States); Richter, Mark M., E-mail: MarkRichter@missouristate.edu [Department of Chemistry, Missouri State University, Springfield, Missouri 65897 (United States)

2012-03-15

Quenching of Ru(bpy) {sub 3}{sup 2+} (bpy=2,2 Prime -bipyridine) coreactant electrogenerated chemiluminescence (ECL) has been observed in the presence of acetaminophen, salicylic acid and related complexes. However, no quenching is observed with the acetylsalicylic acid. In most instances, quenching is observed with 100-fold excess of quencher (compared to ECL luminophore) with complete quenching observed between 10,000 and 100,000 fold excess. Fluorescence and UV-vis experiments coupled with bulk electrolysis support the formation of benzoquinone products upon electrochemical oxidation. The mechanism of quenching may involve the interaction of the electrochemically generated benzoquinone species with (i) the {sup Low-Asterisk }Ru(bpy){sub 3}{sup 2+} excited state or (ii) highly energetic coreactant radicals. - Highlights: Black-Right-Pointing-Triangle Efficient quenching of the electrogenerated chemiluminescence is observed. Black-Right-Pointing-Triangle Acetaminophen, salicylic acid and related compounds can be detected. Black-Right-Pointing-Triangle The mechanism of quenching involves benzoquinones formed upon electrolysis.

Biotechnological production and applications of the omega-3 polyunsaturated fatty acid docosahexaenoic acid

NARCIS (Netherlands)

Sijtsma, L.; Swaaf, de M.E.

2004-01-01

Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid composed of 22 carbon atoms and six double bonds. Because the first double bond, as counted from the methyl terminus, is at position three, DHA belongs to the so-called omega-3 group. In recent years, DHA has attracted much attention because

Emirhan Yeniki’nin “Bir Saatliğine” Hikâyesi Ve Tatar Edebiyatında Savaşın İzleri “For One Hour” Story Of Emirhan Yeniki And The Effects Of War In Tatar Literature

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Alsu KAMALİEVA

2013-03-01

Full Text Available War offers a variety of themes for artists with the tragedies it embodies. The war theme is one of the most challenging themes of literary works. Wars that have been experienced throughout the history of humanity have been worked in various literary genres in world literatures. “War” has been widely discussed in Tatar Literature as well. This is strongly related to the fact that Tatar society deeply experienced the sufferings of World War II. As it is known, the Tatar people had livedclosely with Soviet people and struggled against the Nazi Germany inthe Second World War by taking sides with Soviet Union. Many Tatarwriters and artists had been in the war in person and experienced theprocess. EmirhanYeniki, whose name has exceeded the borders of hiscountry, is one of those Tatar artists who experienced the war andreflected it successfully. “Bir Saatliğine” (For One Hour is a significantstory by EmirhanYeniki which established a place for itself in TatarLiterature among the works of war period. In the story, Yeniki tells usabout the Tatar society during the World War II and the meeting of amother with his youngest son of her three children for one hour byenriching the story with psychological analyses. In the “For One Hour”story, we can find about Tatar village life with all its details within thetechnical possibilities of story genre. In addition, the significance of“train”, the means of transportation where the story was set around,among Tatar society is clearly emphasized in the story. Savaş, trajedileriyle, sanatkârlara geniş bir yelpazede tema çeşitliliği verir. Savaş teması, edebi eserlerin de iddialı temalarından biridir. İnsanlık tarihi boyunca yaşanan savaşlar, dünya edebiyatlarında çeşitli edebî türlerde işlenmiştir. Tatar Edebiyatı’nda da savaş teması geniş bir şekilde yer almıştır. Bu durumun ortaya çıkmasında Tatar toplumunun, İkinci Dünya Savaşı’nın bütün acılarını derin

Discovery of a New Class of Sortase A Transpeptidase Inhibitors to Tackle Gram-Positive Pathogens: 2-(2-Phenylhydrazinylidenealkanoic Acids and Related Derivatives

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Benedetta Maggio

2016-02-01

Full Text Available A FRET-based random screening assay was used to generate hit compounds as sortase A inhibitors that allowed us to identify ethyl 3-oxo-2-(2-phenylhydrazinylidenebutanoate as an example of a new class of sortase A inhibitors. Other analogues were generated by changing the ethoxycarbonyl function for a carboxy, cyano or amide group, or introducing substituents in the phenyl ring of the ester and acid derivatives. The most active derivative found was 3-oxo-2-(2-(3,4dichlorophenylhydrazinylidenebutanoic acid (2b, showing an IC50 value of 50 µM. For a preliminary assessment of their antivirulence properties the new derivatives were tested for their antibiofilm activity. The most active compound resulted 2a, which showed inhibition of about 60% against S. aureus ATCC 29213, S. aureus ATCC 25923, S. aureus ATCC 6538 and S. epidermidis RP62A at a screening concentration of 100 µM.

Türk Yazınından Seçili İki Romanda Göç ve Göçmen Olgusunun Paydaşlık Bağlamında Yansımaları

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Birkan KARGI

2017-09-01

Full Text Available Nazan Bekiroğlu’nun 2012 yılında yayınlanan Nar Ağacı romanı ve Ali Arslan’ın 2003 yılında yayınlanan Ama Sevgi Kalmalı adlı romanda, anlatıcılar kendi köklerini ve kendilerinden önceki kuşakların göç yazgılarının izlerini sürerler. Bu yapıtlarda biri batıdan doğuya, diğeri ise doğudan batıya evlerinden ayrılarak göç etmek zorunda kalan protogonistlerin yaşam öyküleri ele alınmıştır. Nar Ağacı romanında protogonistin göç serüveni İran’ın Tebriz kentinden başlayıp Tiflis, Bakü, Batum ve Trabzon zincirinde gelişirken, Ama Sevgi Kalmalı adlı roman da protogonistin göç serüveni Hannover’in Linden bölgesinden Filistin’e oradan da Anadolu’ya, Egenin Seki köyüne kadar devam eder. Yapıtların arka dokusunu Rus işgali, Birinci Dünya Savaşı, Milli Mücadele ve sonrası oluşturur. Göç serüveninde, göçmenlerin kimlikleri bölünür, parçalanır ve kendilerini yeniden gerçekleştirmek zorunda hissederler. Bu bağlamda Hans’ın Sofiya’ya, Settarhan’ın Zehra’ya olan aşkları protogonistler için varoluşsal bir işlev görür. Aslında her iki yapıttaki protogonistlerin göç nedenlerini, kurulu düzene ‘karşı olma’ ve kendi farklılıklarını ortaya koyma üzerine kuruludur. Derrida’nın da vurguladığı gibi, kimlikler ancak kendi farklılıklarıyla var olabilir ve bir anlamda her kimlik ötekidir. Benzer şekilde Laclau’ya göre de tüm kimliklerin temelinde antagonizma ve dışlanma bulunur. Hans, savaş karşıtı duruşuyla Almanya’da ötekidir. Settarhan ise gelenek ve törelere karşı duruşuyla öteki olmak durumuna düşmüştür. Her ikisi de göç nedeniyle yeniden varlık sorunlarını biçimlendirmeye çalışırlar. Her iki yapıtta da göç, benzer nedenlere dayanmaktadır. Bu benzerlik, batı ve doğu dünyasının karşıtlığında ortaya çıkar. Avrupa’da Aydınlanma anlayışının gelişim sürecinde egemen olan modernite

Nâbî'nin Münşeâtında Biyografik Bilgiler Biographical Accounts In Nabi’s Munsheat

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Halil İbrahim HAKSEVER

2013-03-01

Full Text Available An eminent representative of erudite poetry, Nabi is compiled several prosal and poetic works. Born in Edessa, he had been to Istanbul before living for a long time in Aleppo. He turned back toIstanbul and died there at the age of 70. He has been remembered for afamous and wise poet. Among his works, his Munsheaat, whichincludes monobiographical accounts about his life, is a significant book.His letters mostly written to his friends especially in Aleppo gives usbrief accounts and clues about literary aspects of his personality.Analysis of these letter helps us to have knowledge about his relationswith the bureaucracy elites around him and the civil servants on theone hand; his expectations, his appointments, difficult life conditions helived in. In these letters, there are some remarks about the problemsrelated to official institutions of his time, civil unrest in some regions.There are also mention of some of his appointments, official duties,psychological problems he suffered from, his endeavours to send hisworks to the officials and friends. In this article, using the publishedversion of the Munsheat, we will try to quote and analyze some parts ofthese letters. We will also quote and discuss some passages giving usinformation about Nabi, his ideas and observations. Hikemî şiirin büyük temsilcisi Nabî, manzum mensur birçok eserin sahibidir. Urfa’da başlayan hayatı İstanbul’da devam etmiş, çeşitli memuriyetlerde bulunmuş, uzun yıllar Halep’te yaşamıştır.Uzun gurbet hayatından sonra tekrar İstanbul���a dönmüş, yetmiş yaşını aşmış olarak itibarlı, şöhretli, hikmetli bir şair kimliğiyle hayata veda etmiştir.Yazdığı eserler arasında, hayatına dair kendi kaleminden aktarma bilgiler bulunan Münşeatı önemlidir. Daha çok İstanbul dışında -özellikle Halep’te- iken dost ve tanıdıklarına yazdığı çeşitli konulardaki mektupları, Nabi’nin sosyal ve edebî hayatına dair k

İş Hayatında Tükenmişlik Sendromu: Finans ve Muhasebe Çalışanlarının Tükenmişlik Düzeylerinin Belirlenmesine İlişkin Bir Çalışma

Directory of Open Access Journals (Sweden)

Elvan OKUTAN

2013-09-01

Full Text Available Çalışmanın amacı, KOBİ’lerin Finans ve Mali İşler Departmanı'nda çalışan muhasebe ve finans elemanlarının tükenmişlik düzeyinin belirlenmesi ve tükenmişlik düzeyleri ile demografik özellikleri arasındaki ilişkinin tespit edilmesidir. Araştırma kapsamında uygulanan ankette, Maslach Tükenmişlik ölçeği kullanılmıştır. Elde edilen veriler SPSS 15 kullanılarak frekans değerleri, t-testi ve ANOVA testi yardımıyla analiz edilmiştir. Araştırmadan elde edilen bulgular değerlendirildiğinde genel olarak araştırma kapsamındaki meslek elemanlarının duygusal tükenme düzeylerinin düşük, duyarsızlaşma düzeylerinin normal, kişisel başarı düzeylerinin ise yüksek olduğu saptanmıştır. Bu bağlamda, yapılan araştırma kapsamında muhasebe ve finans çalışanlarının düşük tükenme düzeylerine sahip oldukları ifade edilebilir.

AcEST: BP913801 [AcEST

Lifescience Database Archive (English)

Full Text Available YMU001_000035_D11 562 Adiantum capillus-veneris mRNA. clone: YMU001_000035_D11. BP913801... CL482Contig1 Show BP913801 Clone id YMU001_000035_D11 Library YMU01 Length 562 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000035_D11. Accession BP913801 Tissue type prothallium Developmental stage...tabase search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913801|Adiantum...earch programs, Nucleic Acids Res. 25:3389-3402. Query= BP913801|Adiantum capillus-veneris mRNA, clone: YMU0

Delice(Olea europea var. oleaster L.) ile zeytin (Olea europea var.sativa) arasında anatomik ve palinojik ayrıcalıklar (The Anatomic And Palynological Differences Between Olea europea var. oleaster L. AND Olea europea var.sativa)

OpenAIRE

Kaya, Zafer

1991-01-01

Delice(Olea europea var. oleaster L.) ile zeytin (Olea europea var.sativa) arasında anatomik ve palinojik ayrıcalıklar (The Anatomic And Palynological Differences Between Olea europea var. oleaster L. AND Olea europea var.sativa)

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014 . Scientific Opinion on the substantiation of a health claim related to zinc and normal growth pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion related to a notification from the Oenological Products and Practices International Association (OENOPPIA) on lysozyme from hen‟s egg to be used in the manufacture of wine as an anti-microbial stabilizer

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion related to a notification from the Oenological Products and Practices International Association (OENOPPIA) on lysozyme from hen‟s egg used in t...... reactions in susceptible individuals under the conditions of use proposed by the applicant. © European Food Safety Authority, 2011...... in the manufacture of wine as an anti-microbial stabilizer/additive pursuant to Article 6, paragraph 11 of Directive 2000/13/EC – for permanent exemption from labelling. Allergic sensitisation against lysozyme is common among egg allergic individuals. In winemaking, lysozyme is used for the control of lactic acid...... individuals have been demonstrated in wines treated with lysozyme, and a number of clinical reports (including one double-blind placebo-controlled food challenge with lysozyme) described clinical allergic reactions to lysozyme. The Panel concludes that wines treated with lysozyme may trigger adverse allergic...

Yazma Eğitimi Alanında Yapılan Akademik Çalışmaların Eğilimleri (2010 - 2014 Yılları

Directory of Open Access Journals (Sweden)

Mehmet TOK

2015-10-01

Full Text Available Yazma becerisinin birçok bilişsel işlemleri içerisinde barındırması, süreç içermesi, öğrencinin psikolojik durumu, motivasyon ve tutumu, öğretmenlerin yazma alanındaki konumu vb. unsurlar yazma alanında birçok sorunun yaşanmasını beraberinde getirmektedir. Bu nedenle, alan yazında yer alan birçok çalışmada, yazma sorunları doğrudan ele alınmış ya da sıkça bu sorunlara değinilmiştir. Yazma alanındaki sorunların çok olması, alanda yapılan çalışmaların önemini bir kat daha arttırmıştır. Bu çalışmada, yazma eğitimi konusunda 2010-2014 yılları arasında yapılan 126 yüksek lisans, 38 doktora tezinin yanı sıra düzenli bir şekilde yayınlanan 16 ulusal ve uluslararası bilimsel dergide yer alan 127 makale taranmıştır. Ele alınan akademik çalışmalar; hedef kitle, ilişkili olduğu alan, kullanılan yöntem ve yazma eğitimindeki eğilimleri bakımından değerlendirilmiştir. Araştırmada meta-analiz yöntemi kullanılmıştır. Yapılan araştırmalarda hedef kitle bakımından en fazla ortaokul öğrencilerinin seçildiği, yazmanın daha çok okuma ve ilk okuma ile ilişkilendirilerek ele alındığı, yöntem olarak nicel araştırmaların (f=152 nitel araştırmalardan (f=114 daha çok tercih edildiği görülmüştür. En çok kullanılan yöntem tarama modeli olurken doktora tezlerinde deneysel çalışmalar ön plana çıkmıştır. Yazma araştırmaları incelendiğinde araştırmacı eğilimlerinin, yazma yöntemleri ve farklı türlerde metin yazmaya yönelik olduğu görülmüştür.

Process development of oxalic acid production in submerged culture of Aspergillus niger F22 and its biocontrol efficacy against the root-knot nematode Meloidogyne incognita.

Science.gov (United States)

Lee, Sang Il; Lee, Keon Jin; Chun, Ho Hyun; Ha, Sanghyun; Gwak, Hyun Jung; Kim, Ho Myeong; Lee, Jong-Hee; Choi, Hak-Jong; Kim, Hyeong Hwan; Shin, Teak Soo; Park, Hae Woong; Kim, Jin-Cheol

2018-03-01

Oxalic acid has potent nematicidal activity against the root-knot nematode Meloidogyne incognita. In this study, fermentation parameters for oxalic acid production in submerged culture of Aspergillus niger F22 at 23, 25, and 30 °C were optimized in 5-L jar fermenters. The viscosity of the culture broth increased with increasing temperature. There was a negative correlation between oxalic acid production and the apparent viscosity; high volumetric productivity of oxalic acid was obtained at low apparent viscosity (less than 1000 cP), with a productivity of more than 100 mg/L h. When the apparent viscosity was over 2500 cP, the volumetric productivity decreased below 50 mg/L h. In addition, the volumetric mass transfer coefficient, K L a, positively correlated with volumetric productivity. When the K L a value increased from 0.0 to 0.017 /s, the volumetric productivity proportionally increased up to 176 mg/L h. When the temperature decreased, K L a increased due to the decrease in viscosity, leading to increased volumetric productivity. The highest productivity of 7453.3 mg/L was obtained at the lowest temperature, i.e., 23 °C. The nematicidal activity of culture filtrate was proportional to the content of oxalic acid. Based on a constant impeller tip speed, oxalic acid production was successfully scaled up to a 500-L pilot vessel, producing a final concentration comparable to that in the 5-L jar.

İşitme Kayıplı Çocuğu Olan Bir Annenin Etkileşim Davranışlarının Aile Eğitimi Bağlamında İncelenmesi

Directory of Open Access Journals (Sweden)

Emel Ertürk Mustul

2016-04-01

Full Text Available Bu eylem araştırmasında çok ileri dereceli işitme kayıplı çocuğu olan işiten bir annenin etkileşim davranışları ve bu davranışlarının desteklenme biçiminin aile eğitimi bağlamında incelenmesi amaçlanmıştır. Araştırmanın verileri aile eğitimi video kayıtları, anneyle yapılan görüşmeler, yazarlar arasında yapılan aile eğitimi değerlendirmeleri ile geçerlik güvenirlik toplantılarında alınan ses kayıtları, aile eğitimi seans planları ve araştırmacı günlükleriyle toplanmıştır. Toplam sekiz aile eğitimi yapılmıştır ve annenin etkileşim davranışlarını incelemek amacıyla ikinci, dördüncü, altıncı ve sekizinci aile eğitimlerinde yer alan on dakikalık anne-çocuk etkileşimi bölümleri temsili video kaydı olarak belirlenmiş ve tümevarım analizi uygulanmıştır. Analiz sonucunda beş etkileşim davranışı belirlenmiĢ ve bu davranışlar iki tema altında toplanmıştır. Annenin etkileşim davranışları bu davranış tanımları altında incelenmiş, davranışların görülme sıklığına bakılmış ve bu davranışları desteklemeye dair eylem planları geliştirilmiştir. Araştırmanın bulguları annenin etkileşimi destekleyen davranışlarının arttığını, etkileşimi olumsuz etkileyen davranışlarının da azaldığını göstermiştir. Araştırma sonuçlarını genellemek mümkün olmasa da elde edilen bulguların etkileşime odaklanan aile eğitimi programının bu anne-çocuk çifti için işlediğini ve erken çocukluk dönemi boyunca aile eğitiminin devam etmesi gerektiğini gösterdiği ileri sürülebilir. The main purpose of this action research was to examine the interactive behaviour of a mother who has a child with a hearing loss and the way these skills were supported in the context of parent guidance process. The data were collected by the video recordings of the parent guidance sessions, audio recordings of session evaluation meetings among

Uşak İli Buğday Ekim Alanlarında Süne, [Eurygaster spp. (Hemiptera: Scutelleridae] Yumurtalarının Parazitlenme Oranları ile Buğday Tanelerindeki Emgi Oranları Arasındaki İlişkinin Belirlenmesi

Directory of Open Access Journals (Sweden)

Erdal ZENGİN

2017-06-01

Full Text Available Bu çalışma, 2015-2016 yıllarında Uşak iline bağlı 10 köydeki Süne yumurtalarının parazitlenme oranlarının ve emgili tane sayılarının belirlenmesi ile bu oranlar arasındaki ilişkinin tespit edilmesi amacıyla yapılmıştır. Araştırma sonuçlarına göre, 2015 ve 2016 yıllarındaki en yüksek Süne yumurtası parazitlenme oranı ve emgili tane sayıları sırasıyla %95.4-%1.60, %90.7-%1.05 olarak bulunmuştur. Köylerde yapılan arazi çalışmaları sonucunda elde edilen oranlara çoklu karşılaştırma testi uygulanarak köyler arasındaki farklılıklar belirlenmiştir.  Parazitlenme oranı ile emgili tane sayısı arasında bir ilişkinin olup olmadığını belirlemek için her iki yıl verileri üzerinde yapılan regresyon analizi sonucunda, bu iki değişken arasında doğrusal bir ilişkinin olduğu tespit edilmiştir.

Electrochemical behaviour of cuprous complexes of dithia-alkanedicarboxylic acids

NARCIS (Netherlands)

Pieterse, M.M.J.; Janssen, L.J.J.

1972-01-01

The composition and electrochemical behaviour of the cuprous complexes of dithia-alkanedicarboxylic acids viz., 2,5-dithiahexane-1,6-dicarboxylic acid (I); 3,6 dithiaoctane-1,8-dicarboxylic acid (II); 4,7-dithiadecane-1,10-dicarboxylic acid (III) and 2,2,

Transformation of levoglucosan over H-MCM-22 zeolite and H-MCM-41 mesoporous molecular sieve catalysts

International Nuclear Information System (INIS)

Kaeldstroem, M.; Kumar, N.; Heikkilae, T.; Tiitta, M.; Salmi, T.; Murzin, D. Yu.

2011-01-01

Catalytic transformation of levoglucosan (1-6-anhdyro-β-D-glucopyranose) was carried out in a fixed bed reactor at 573 K over zeolite and mesoporous material catalysts. Proton forms of MCM-22-30 and MCM-41-20 catalysts were tested in the conversion, changing also the residence time. The yield of the transformation product phases was substantially influenced by the structures, at the same time the formation of the different compounds were dependent on the structures of the acidic zeolite catalysts. Oxygenated species were the main liquid product, consisting mainly of aldehydes and furfurals (glycolaldehyde, formaldehyde, acetaldehyde, furfural, 5-methylfurfural, acetic acid). The formation of the liquid products was higher over MCM-41-20 than over MCM-22-30 for all the oxygenated species except acetic acid, indicating larger formation of non-condensable products over the microporous material. By increasing the residence time the formation of acetic acid increased in transformations over MCM-22, however, such increase also led to generation of more gases with both catalysts. The deactivation due to coking was more severe over the zeolite compared to the mesoporous material. It was, however, possible to successfully regenerate the spent zeolites without changing the structure. -- Highlights: → Transformation of levoglucosan (1-6-anhdyro-β-D-glucopyranose) was done at 573 K. → MCM-22-30 and MCM-41-20 catalysts were tested. → Oxygenated species (glycolaldehyde, formaldehyde, acetaldehyde, furfural, 5-methylfurfural, acetic acid) were the main liquid products. → Acidity had an influence on product distribution and deactivation.

An Organic Puzzle Using Meldrum's Acid

Science.gov (United States)

Crouch, R. David; Holden, Michael S.

2002-04-01

Meldrum's acid or 2,2-dimethyl-1,3-dioxane-4,6-dione undergoes a Knoevenagel condensation with formaldehyde to form an active Michael acceptor for a second molecule of Meldrum's acid. The structure of the resulting product is determined by correlation of the products of possible reactions of Meldrum's acid and formaldehyde with the NMR spectrum of the product.

Almanya'da sığır ve dana eti piyasa düzeni ve Türkiye için sığır eti pazarlamasında model oluşturabilme olanakları üzerine bir araştırma

OpenAIRE

KILIÇ, Sevinç

2006-01-01

Almanya'da Sığır ve Dana Eti Piyasa Düzeni ve Türkiye İçin Sığır Eti PazarlamasındaModel Oluşturabilme Olanakları Üzerine Bir AraştırmaBu araştırmada Avrupa Birliği Ortak Tarım Politikası kapsamında Sığır ve Dana Eti OrtakPiyasa Düzeni'nde uygulanmış ve uygulanmakta olan politikalar, Almanya'nın kasaplıkhayvan ve et piyasa yapısı ile ilgili düzenlemeleri, Almanya'da kasaplık hayvan ve etpazarlamasının tarihsel gelişimi detaylı olarak incelenmiştir.Türkiye'de 2001-2004 yıl...

Metabolism of methyl-branched iodo palmitic acids in cultured hepatocytes

International Nuclear Information System (INIS)

Thomas, G.; Pepin, D.; Loriette, C.; Chambaz, J.; Bereziat, G.; Vidal, M.; Apparu, M.; Coornaert, S.

1989-01-01

The metabolic fate of methyl-branched iodo fatty acids was studied in primary culture of rat hepatocytes. We compared 16-iodo-2-R,S-methyl palmitic acid (2-Me), which can be β oxidized, with 16-iodo-3-R,S-methyl palmitic acid (3-Me) which can be β oxidized only after an initial α oxydation and with 16-iodo-2,2-dimethyl palmitic acid (2,2-Me 2 ) and 16-iodo-3,3-dimethyl palmitic acid (3,3-Me 2 ) which cannot be β oxidized at all. The normal fate of natural fatty acids was given by comparative experiments with [1- 14 C] palmitic acid. Monomethyl-branched iodo fatty acids were taken up in the same range as palmitic acid but more than dimethyl-branched iodo fatty acids. After a 15-h incubation, acido-soluble products (ASP) accounted for 75% of the radioactivity taken up as 16-iodo-2-methyl palmitic acid, 50% as other methyl-branched iodo fatty acids and only 30% as palmitic acid. Cultured hepatocytes, labelled for 3 h with the various fatty acids and reincubated for 12 h without fatty acid, secreted large amounts of free dimethyl-branched iodo fatty acids as compared to the monomethyl ones and palmitic acid. Only hepatocytes prelabelled with 16-[ 125 I]iodo-2,2-dimethyl palmitic acid exhibited an appreciable secretion of labeled triglycerides, but at a lower rate than with [1- 14 C] palmitic acid. Conversely, the 16-iodo-monomethyl palmitic acids remained chiefly in hepatocyte triglycerides. Minute amounts of 16-iodo-methyl-branched palmitic acids were found in hepatocyte or secreted phospholipids as compared with palmitic acid. (orig.)

Influence of different metal ions on the ultrastructure, biochemical properties, and protein localization of the K562 cell nuclear matrix.

Science.gov (United States)

Neri, L M; Bortul, R; Zweyer, M; Tabellini, G; Borgatti, P; Marchisio, M; Bareggi, R; Capitani, S; Martelli, A M

1999-06-01

The higher order of chromatin organization is thought to be determined by the nuclear matrix, a mainly proteinaceous structure that would act as a nucleoskeleton. The matrix is obtained from isolated nuclei by a series of extraction steps involving the use of high salt and nonspecific nucleases, which remove chromatin and other loosely bound components. It is currently under debate whether these structures, isolated in vitro by unphysiological extraction buffers, correspond to a nucleoskeleton existing in vivo. In most cell types investigated, the nuclear matrix does not spontaneously resist these extractions steps; rather, it must be stabilized before the application of extracting agents. In this study nuclei, isolated from K562 human erythroleukemia cells, were stabilized by incubation with different metal ions (Ca2+, Cu2+, Zn2+, Cd2+), and the matrix was obtained by extraction with 2 M NaCl. By means of ultrastructural analysis of the resulting structures, we determined that, except for Ca2+, all the other metals induced a stabilization of the matrix, which retained the inner fibrogranular network and residual nucleoli. The biochemical composition, analyzed by two-dimensional gel electrophoresis separation, exhibited a distinct matrix polypeptide pattern, characteristic of each type of stabilizing ion employed. We also investigated to what extent metal ions could maintain in the final structures the original distribution of three inner matrix components, i.e. NuMA, topoisomerase IIalpha, and RNP. Confocal microscopy analysis showed that only NuMa, and, to a lesser extent, topoisomerase IIalpha, were unaffected by stabilization with divalent ions. On the contrary, the fluorescent RNP patterns detected in the resulting matrices were always disarranged, irrespective of the stabilization procedure. These results indicate that several metal ions are powerful stabilizing agents of the nuclear matrix prepared from K562 erythroleukemia cells and also strengthen the

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific opinion on niacin and contribution to normal energy-yielding metabolism: evaluation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to ♀EFAX™ and reduction of menstrual discomfort pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Nutrilinks Sarl submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Cyprus, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific opinion on biotin and contribution to normal energy-yielding metabolism: evaluation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

Asetonitril-Su İkili Karışımlarında Piroksikam ve Tenoksikam'ın Potansiyometrik pKa Tayini

OpenAIRE

Çubuk Demiralay, Ebru; Yılmaz, Hülya

2012-01-01

İyonlaşma sabiti (pKa) çözünebilirlik sınırlaması olmaksızın doğru tahmin edilebilen parametrelerden birisidir. Sunulan bu çalışmada, piroksikam ve tenoksikamın asit- baz davranışı çalışılmıştır. Potansiyometrik metot kullanılarak, piroksikam ve tenoksikamın pKa değerleri asetonitril-su ikili karışımlarının farklı yüzdelerinde tayin edilmiştir (asetonitril içeriği hacimce % 30 ile 45 arasında). Bu bileşiklerin sudaki pKa değerleri mol kesri ve Yasuda-Shedlovsky eşitlikleri kullanarak hesaplan...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for chromium

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

parenteral nutrition was considered to be the most convincing, but overall data do not provide sufficient information on the reversibility of the possible deficiencies and the nature of any dose–response curve in order to identify a dietary requirement for humans. The Panel concludes that no Average......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) considered the evidence for setting Dietary Reference Values for chromium. Trivalent chromium (Cr(III)) has been postulated to be necessary for the efficacy of insulin in regulating...... models have not produced consistent results, and that there is no evidence of essentiality of Cr(III) in animal nutrition. Evaluating the possibility of Cr(III) as an essential element for humans, the evidence from reported improvements associated with chromium supplementation in patients on total...

22 CFR 171.22 - Appeals.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Appeals. 171.22 Section 171.22 Foreign Relations DEPARTMENT OF STATE ACCESS TO INFORMATION AVAILABILITY OF INFORMATION AND RECORDS TO THE PUBLIC Executive Order 12958 Provisions § 171.22 Appeals. Any denial of a mandatory declassification review request...

İmar Meclisi Raporlarında Borçlanma ve Selem Uygulaması (1845-1846 / Borrowing and Selem Practices in Ottoman Era According to "Macalis Imariyya (Development Counsils" Reports

Directory of Open Access Journals (Sweden)

Tahir Bilirli

2016-06-01

Full Text Available İmar Meclisi Raporlarında Borçlanma ve Selem Uygulaması (1845-1846 Öz Bu makale, Osmanlı Devleti'nde 1845 yılında ilan edilen Tensikât-ı mülkiye kararı çerçevesinde geçici olarak olarak kurulan İmar Meclisleri'nin görevlerini incelemektedir. Makalede, bu meclislerin özellikle halkın borç para alma durumları ile selem usulü denilen mahsulün hasattan önce satılması konusundaki faaliyetleri incelenmektedir. Hasat zamanından önce alınan vergi, ahaliyi borçlanmaya yöneltmekte, bu da halkın fahiş faiz oranlarıyla tefecilerden borç almalarına ve ürünün hasat edilmeden önce daha düşük fiyatlara satılmasına sebep olmaktaydı. Bu durum gelir kaybı nedeniyle hem tebaya hem de devlete zarar vermekteydi. İmar Meclisi memurları yaşanan bu sıkıntının giderilmesi noktasında yerinde durum tespiti yapmışlardır. Makalenin birinci bölümünde İmar Meclisleri'nin kuruluşu ve görevleri hakkında bilgi verilmektedir. İkinci bölümde makalenin ana konusu olan borçlanma ve selem usulünün uygulanmasının meclis raporlarına nasıl yansıdığı arşiv belgelerine göre ele alınmıştır. Makalenin sonuç bölümünde arşiv belgelerinde elde edilen veriler doğrultusunda değerlendirme yapılmıştır. Borrowing and Selem Practices in Ottoman Era According to "Macalis Imariyya (Development Counsils" Reports Abstract This article examines the mission of Macalis Imariyya (Develepment Counsils which were founded by the decree of Tankisat al-Mulkiyye declared in 1845. In the article, the activity of these Counsils about the indebtedness status of the people and the selling of products before the harvest -called “selem”- particularly examined. The tax collection before harvest period was impelling the people borrowing from usurers with excessive interest rates and to sell the product at nearly half price. This case was damaging both the state and the citizens because of income loss. Development Council

Spectrophotometric study into complexing of vanadium(3) with salicylic acid derivatives

Energy Technology Data Exchange (ETDEWEB)

Dolgorev, A V; Serikov, Yu A; Zolotavin, V L

1977-03-01

Complexing of vanadium (3) with 5 amino-salicylic acid and amide of salicylhydroxamic acid has been studied. It has been shown that in acidic medium V/sup 3 +/ forms yellow complexes of the composition 1:1 with instability constants 2.2x10/sup -19/, 7.8x10/sup -11/, and 2.2x10/sup -12/, respectively. Complexes of V/sup 3 +/ with derivatives of salicylic acid can be used for determining V(3) content in the presence of V(4).

H ferritin silencing induces protein misfolding in K562 cells: A Raman analysis

KAUST Repository

Zolea, Fabiana

2015-10-09

The redox state of the cell is involved in the regulation of many physiological functions as well as in the pathogenesis of several diseases, and is strictly dependent on the amount of iron in its catalytically active state. Alterations of iron homeostasis determine increased steady-state concentrations of Reactive Oxygen Species (ROS) that cause lipid peroxidation, DNA damage and altered protein folding. Ferritin keeps the intracellular iron in a non-toxic and readily available form and consequently plays a central role in iron and redox homeostasis. The protein is composed by 24 subunits of the H- and L-type, coded by two different genes, with structural and functional differences. The aim of this study was to shed light on the role of the single H ferritin subunit (FHC) in keeping the native correct protein three-dimensional structure. To this, we performed Raman spectroscopy on protein extracts from K562 cells subjected to FHC silencing. The results show a significant increase in the percentage of disordered structures content at a level comparable to that induced by H2O2 treatment in control cells. ROS inhibitor and iron chelator were able to revert protein misfolding. This integrated approach, involving Raman spectroscopy and targeted-gene silencing, indicates that an imbalance of the heavy-to-light chain ratio in the ferritin composition is able to induce severe but still reversible modifications in protein folding and uncovers new potential pathogenetic mechanisms associated to intracellular iron perturbation.

H ferritin silencing induces protein misfolding in K562 cells: A Raman analysis

KAUST Repository

Zolea, Fabiana; Biamonte, Flavia; Candeloro, Patrizio; Di Sanzo, Maddalena; Cozzi, Anna; Di Vito, Anna; Quaresima, Barbara; Lobello, Nadia; Trecroci, Francesca; Di Fabrizio, Enzo M.; Levi, Sonia; Cuda, Giovanni; Costanzo, Francesco

2015-01-01

The redox state of the cell is involved in the regulation of many physiological functions as well as in the pathogenesis of several diseases, and is strictly dependent on the amount of iron in its catalytically active state. Alterations of iron homeostasis determine increased steady-state concentrations of Reactive Oxygen Species (ROS) that cause lipid peroxidation, DNA damage and altered protein folding. Ferritin keeps the intracellular iron in a non-toxic and readily available form and consequently plays a central role in iron and redox homeostasis. The protein is composed by 24 subunits of the H- and L-type, coded by two different genes, with structural and functional differences. The aim of this study was to shed light on the role of the single H ferritin subunit (FHC) in keeping the native correct protein three-dimensional structure. To this, we performed Raman spectroscopy on protein extracts from K562 cells subjected to FHC silencing. The results show a significant increase in the percentage of disordered structures content at a level comparable to that induced by H2O2 treatment in control cells. ROS inhibitor and iron chelator were able to revert protein misfolding. This integrated approach, involving Raman spectroscopy and targeted-gene silencing, indicates that an imbalance of the heavy-to-light chain ratio in the ferritin composition is able to induce severe but still reversible modifications in protein folding and uncovers new potential pathogenetic mechanisms associated to intracellular iron perturbation.

The auto-orientation in high magnetic fields of oxidized cytochrome b562 as source of constraints for solution structure determination

International Nuclear Information System (INIS)

Arnesano, Fabio; Banci, Lucia; Bertini, Ivano; Wetering, Karin van der; Czisch, Michael; Kaptein, Robert

2000-01-01

15 N- 1 H 1 J couplings were measured at 500 MHz and 800 MHz for 15 N enriched oxidized cytochrome b 562 from E. coli. The magnetic field dependence of 70 1 J values, which could be measured without signal overlap, shows that there is a molecular magnetic anisotropy which provides partial molecular orientation in the magnetic field and, consequently, residual dipolar couplings (rdc). The rdc were used as further constraints to improve the existing structure [Arnesano et al. (1999) Biochemistry, 38, 8657-8670] with a protocol which uses the rhombic anisotropy [Banci et al. (1998) J. Am. Chem. Soc., 120, 12903-12909]. The overall large molecular magnetic anisotropy has been found to be determined by both the low spin iron (III) and the four helix bundle structure magnetic susceptibility anisotropy contributions

The Cytotoxicity of Benzaldehyde Nitrogen Mustard-2-Pyridine Carboxylic Acid Hydrazone Being Involved in Topoisomerase IIα Inhibition

Directory of Open Access Journals (Sweden)

Yun Fu

2014-01-01

Full Text Available The antitumor property of iron chelators and aromatic nitrogen mustard derivatives has been well documented. Combination of the two pharmacophores in one molecule in drug designation is worth to be explored. We reported previously the syntheses and preliminary cytotoxicity evaluation of benzaldehyde nitrogen mustard pyridine carboxyl acid hydrazones (BNMPH as extended study, more tumor cell lines (IC50 for HepG2: 26.1 ± 3.5 μM , HCT-116: 57.5 ± 5.3 μM, K562: 48.2 ± 4.0 μM, and PC-12: 19.4 ± 2.2 μM were used to investigate its cytotoxicity and potential mechanism. In vitro experimental data showed that the BNMPH chelating Fe2+ caused a large number of ROS formations which led to DNA cleavage, and this was further supported by comet assay, implying that ROS might be involved in the cytotoxicity of BNMPH. The ROS induced changes of apoptosis related genes, but the TFR1 and NDRG1 metastatic genes were not obviously regulated, prompting that BNMPH might not be able to deprive Fe2+ of ribonucleotide reductase. The BNMPH induced S phase arrest was different from that of iron chelators (G1 and alkylating agents (G2. BNMPH also exhibited its inhibition of human topoisomerase IIα. Those revealed that the cytotoxic mechanism of the BNMPH could stem from both the topoisomerase II inhibition, ROS generation and DNA alkylation.

Omega-3 Polyunsaturated Fatty Acids and Their Health Benefits.

Science.gov (United States)

Shahidi, Fereidoon; Ambigaipalan, Priyatharini

2018-03-25

Omega-3 polyunsaturated fatty acids (PUFAs) include α-linolenic acid (ALA; 18:3 ω-3), stearidonic acid (SDA; 18:4 ω-3), eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3). In the past few decades, many epidemiological studies have been conducted on the myriad health benefits of omega-3 PUFAs. In this review, we summarized the structural features, properties, dietary sources, metabolism, and bioavailability of omega-3 PUFAs and their effects on cardiovascular disease, diabetes, cancer, Alzheimer's disease, dementia, depression, visual and neurological development, and maternal and child health. Even though many health benefits of omega-3 PUFAs have been reported in the literature, there are also some controversies about their efficacy and certain benefits to human health.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to “Femilub” and maintenance of vaginal moisture pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Nutrilinks Sarl submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to provide a scientific opinion...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to caffeine and increased alertness pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from SmithKline Beecham Limited, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of United Kingdom, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver...

Ölçüm Öncesi Dinlenme Süresinin ve Dinlenme Esnasında Konuşmanın İndirekt Kan Basıncı Ölçüm Değeri Üzerine Etkisi

OpenAIRE

BAYRAKTAR, DUYGU; KHORSHTD, LEYLA

2018-01-01

Başlık:Ölçüm Öncesi Dinlenme Süresinin ve DinlenmeEsnasında Konuşmanın İndirekt Kan Basıncı Ölçüm Değeri Üzerine EtkisiTitle:Özet:            Amaç: Buaraştırma, indirekt arteriyel kan basıncı ölçerken bireyin dinlenme süresininve dinlenme esnasında konuşmanın ölçüm değeri üzerine etkisini incelemekamacıyla yapılmıştır.Gereç-Yöntem:Örneklemini, bir üniversite hastanesinin İç Hastalıkları Polikliniği’nebaşvuran 18 yaşın üzerinde olan, 60 normotansif ve 60 hipertansif bireyleroluşturmuştur. Veri...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to choline and “development of brain” pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

Muhasebe Meslek Mensuplarında Tükenmişlik Sendromu Üzerine Bir Araştırma

OpenAIRE

DOĞAN, Prof. Dr. Zeki; NAZLIOĞLU, Elif Hilal

2010-01-01

Bu çalışmanın amacı, muhasebe meslek mensuplarının tükenmişlik sendromu yaşayıp-yaşamadıklarını incelemek ve eğer yaşıyorlarsa bunun boyutunu araştırmaktır. Bunun için Kayseri ilinde faaliyet gösteren muhasebe meslek mensuplarının tükenmişlik düzeylerinin ölçümüne ve tükenmişliğin alt boyutlarında nedensellik ilişkilerinin yönünün tespitine ilişkin bir anket çalışması yapılmıştır. Elde edilen verilerden hareketle ilk olarak Maslach Tükenmişlik Ölçeği hesaplanmış ve daha sonra nedensellik test...

Mutual diffusion coefficients of L-glutamic acid and monosodium L-glutamate in aqueous solutions at T = 298.15 K

International Nuclear Information System (INIS)

Ribeiro, Ana C.F.; Rodrigo, M.M.; Barros, Marisa C.F.; Verissimo, Luis M.P.; Romero, Carmen; Valente, Artur J.M.; Esteso, Miguel A.

2014-01-01

Highlights: • Interdiffusion coefficients of L-glutamic acid and sodium L-glutamate were measured. • The L-glutamic acid behaves as a monoprotic weak acid. • The sodium L-glutamate shows a symmetrical 1:1 non-associated behaviour. • Limiting diffusion coefficients and ionic conductivities were estimated. • Diffusion coefficients were discussed on the basis of the Onsager–Fuoss equations. - Abstract: Mutual diffusion coefficient values for binary aqueous solutions of both L-glutamic acid (H 2 Glu) and sodium L-glutamate (NaHGlu) were measured with the Taylor dispersion technique, at T = 298.15 K, and concentrations ranging from (0.001 to 0.100) mol · dm −3 . The results were discussed on the basis of the Onsager–Fuoss and the Nernst theoretical equations, by considering the H 2 Glu as a weak acid (monoprotic acid, with K 2 = 5.62 · 10 −5 ). The smaller values found for the acid with respect to those of the salt, confirm this association hypothesis. From the diffusion coefficient values at infinitesimal concentration, limiting ionic conductivities as well as the hydrodynamic radius of the hydrogen glutamate ion (HGlu − ) were derived and analyzed in terms of the chain methylene groups. The effect of different phenomena, such as association or complexation, were also taken into consideration and discussed. Values for the dissociation degree for H 2 Glu were also estimated

A chromene and prenylated benzoic acid from Piper aduncum.

Science.gov (United States)

Baldoqui, D C; Kato, M J; Cavalheiro, A J; Bolzani, V da S; Young, M C; Furlan, M

1999-08-01

In addition to nerolidol, 2',6'-dihydroxy-4'-methoxydihydrochalcone, methyl 2,2-dimethyl-8-(3'-methyl-2'-butenyl)-2H-1-chromene-6-carboxylate, methyl 2,2-dimethyl-2H-1-chromene-6-carboxylate and methyl 8-hydroxy-2,2-dimethyl-2H-1-chromene-6-carboxylate, two new natural products were isolated from the leaves of Piper aduncum, 2,2-dimethyl-2H-1-chromene-6-carboxylic acid and 3-(3',7'-dimethyl-2',6'-octadienyl)-4-methoxybenzoic acid. The structures of the isolates were established based on analysis of spectroscopic data, including ES-MS. The DNA-damaging activity of the isolated compounds was also investigated against mutant strains of Saccharomyces cerevisiae.

Toxicological evaluation of the flavour ingredient 4-amino-5-(3-(isopropylamino-2,2-dimethyl-3-oxopropoxy-2-methylquinoline-3-carboxylic acid

Directory of Open Access Journals (Sweden)

Amy J. Arthur

2015-01-01

Full Text Available A toxicological evaluation of 4-amino-5-(3-(isopropylamino-2,2-dimethyl-3-oxopropoxy-2-methylquinoline-3-carboxylic acid(S9632; CAS 1359963-68-0, a flavour with modifying properties,was completed for the purpose of assessing its safety for use in food and beverage applications. No Phase I biotransformations of S9632 were observed in rat or human microsomes in vitro, and in rat pharmacokinetic studies, the compound was poorly orally bioavailable and rapidly eliminated. S9632 was not found to be mutagenic or clastogenic in vitro, and did not induce micronuclei or indicate interactions with the mitotic spindle in an in vivo mouse micronucleus assay at oral doses up to 2000 mg/kg. In subchronic oral toxicity studies in rats, the NOEL was 100 mg/kg/day (highest dose tested for S9632 when administered as a food ad-mix for 90 consecutive days. Furthermore, S9632 demonstrated a lack of maternal toxicity, as well as adverse effects on fetal morphology at the highest dose tested, providing a NOEL of 1000 mg/kg/day for both maternal toxicity and embryo/fetal development when administered orally during gestation to pregnant rats.

Kerogen-bound and free hopanoic acids in the messel oil shale kerogen.

Science.gov (United States)

Abbott, G D; Bashir, F Z; Sugden, M A

2001-08-01

The distribution of the free and bound hopanoic acids in both unheated and heated (350 degrees C for 50 h) kerogens, isolated from the Messel oil shale, were analyzed by GC-MS. The bound acids were released by subjecting the kerogen to three different treatments, namely, thermochemolysis in the presence of tetramethylammonium hydroxide (TMAH), as well as basic and acidic hydrolyses. All of these methods gave a series of hopanoic acids ranging from C(30) to C(34), in which the biological 17beta, 21beta(H) configuration is prominent. Both 22R and 22S epimers are present for the C(30) acid, whereas the others are dominated by the sidechain 22R-configuration. Thermochemolysis in the presence of TMAH was the most efficient in releasing kerogen-bound hopanoids. Following pyrolysis, the acids are generated and released into the free fraction with apparent epimerization occurring at C-17, C-21, and C-22. The bound hopanoic acids may be both chemically bonded as well as possibly being physically encapsulated within the macromolecular fraction of sedimentary organic matter. They are therefore either generated by breaking the bonds which bind them to the kerogen or they are released as a result of the macromolecular cage being broken apart. Copyright 2001 Wiley-Liss, Inc.

Symbiotic zooxanthellae provide the host-coral Montipora digitata with polyunsaturated fatty acids.

Science.gov (United States)

Papina, M; Meziane, T; van Woesik, R

2003-07-01

We compared the fatty acid composition of the host-coral Montipora digitata with the fatty acid composition in the coral's endosymbiotic dinoflagellates (zooxanthellae). Fatty acids as methyl esters were determined using gas chromatography (GC) and verified by GC-mass spectrometry. We found the main difference between the fatty acids in the host and their symbionts were that zooxanthellae supported higher proportions of polyunsaturated fatty acids. The presence of fatty acids specific to dinoflagellates (i.e. 18:4omega3, 22:5omega3 and 22:6omega3) in the host tissue suggests that zooxanthellae provide the coral host not only with saturated fatty acids, but also with diverse polyunsaturated fatty acids.

AcEST: BP914406 [AcEST

Lifescience Database Archive (English)

Full Text Available YMU001_000058_E09 562 Adiantum capillus-veneris mRNA. clone: YMU001_000058_E09. BP914406... CL513Contig1 Show BP914406 Clone id YMU001_000058_E09 Library YMU01 Length 562 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000058_E09. Accession BP914406 Tissue type prothallium Developmental stage...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914406|Adiantum capillus...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914406

SYNTHESIS, CRYSTAL STRUCTURE AND LUMINESCENT PROPERTY OF A DINUCLEAR Tb(II COMPLEX WITH HOMOPHTHALIC ACID AND 2,2’-BIPYRIDYL

Directory of Open Access Journals (Sweden)

LI-HUA WANG

2015-07-01

Full Text Available A novel dinuclear Tb(III complex, [Tb(bpy2L2] (bpy = 2,2’-bipyridine, H2L = homophthalic acid, has been synthesized and characterized by elemental analysis and single-crystal X-ray diffraction. The Tb(III complex is monoclinic, space group P21/c with a = 9.368(2 Å, b = 15.948(4 Å, c = 12.216(3 Å, β = 103.023(4º, V= 1778.2(7 Å3, Z = 2, Dc = 1.910 mg·m-3, μ = 4.011 mm-1, F(000 = 996, and final R1 = 0.0602, ωR2 = 0.2192. The result shows that the Tb(III center is seven-coordination with a N2O5 distorted pengonal bipyramidal geometry. The luminescent property of Tb(III complex was investigated.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to Cynatine ® and maintenance of normal joint mobility pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Roxlor Nutra LLC, submitted pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to iodine and contribution to normal cognitive development pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to iodine and contribution to normal thyroid function pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to “complex carbohydrates” and “contribute to satiety” pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to prunes and contribution to normal bowel function pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to OptiEFAX™ and maintenance of normal blood concentrations of triglycerides pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Nutrilinks Sarl, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion...

Profile of Fatty Acids, Amino Acids, Carotenoid Total, and α-Tocopherol from Flying Fish Eggs

Directory of Open Access Journals (Sweden)

Aulia Azka

2015-12-01

Full Text Available Flying fish are found in waters of eastern Indonesia, which until now is still limited information about nutritional content. The purpose of this research was determine the composition of fatty acids, amino acids, total carotenoids, α-tocopherol flying fish eggs (Hyrundicthys sp.. The composition of fatty acid was measured by gas chromatography (GC, while amino acids, total carotenoids, α-tocopherol was measured by High performanced Liquid Chromatography (HPLC. Egg contained 22 fatty acids such as saturated fatty acid 29.71%, monounsaturated fatty acid 7.86%, and polysaturated fatty acid 13.64%. The result showed that eggs flying fish contained 17 amino acids, such as essential amino acid 14.96% and non-essential amino acids 20.27%. Eggs contained a total carotenoid of 245.37 ppm. α-tocopherol content of flying fish eggs by 1.06 ppm.

Biocompatibility of metal injection molded versus wrought ASTM F562 (MP35N) and ASTM F1537 (CCM) cobalt alloys.

Science.gov (United States)

Chen, Hao; Sago, Alan; West, Shari; Farina, Jeff; Eckert, John; Broadley, Mark

2011-01-01

We present a comparative analysis between biocompatibility test results of wrought and Metal Injection Molded (MIM) ASTM F562-02 UNS R30035 (MP35N) and F1537 UNS R31538 (CCM) alloy samples that have undergone the same generic orthopedic implant's mechanical, chemical surface pre-treatment, and a designed pre-testing sample preparation method. Because the biocompatibility properties resulting from this new MIM cobalt alloy process are not well understood, we conducted tests to evaluate cytotoxicity (in vitro), hemolysis (in vitro), toxicity effects (in vivo), tissue irritation level (in vivo), and pyrogenicity count (in vitro) on such samples. We show that our developed MIM MP35N and CCM materials and treatment processes are biocompatible, and that both the MIM and wrought samples, although somewhat different in microstructure and surface, do not show significant differences in biocompatibility.

Divergent Synthesis of Solanidine and 22-epi-Solanidine.

Science.gov (United States)

Hou, Ling-Li; Shi, Yong; Zhang, Zhi-Dan; Wu, Jing-Jing; Yang, Qing-Xiong; Tian, Wei-Sheng

2017-07-21

A divergent synthesis of solanidine and 22-epi-solanidine, two 25S natural steroidal alkaloids, from 25R-configured diosgenin acetate, is described. Initially, solanidine was synthesized through a series of transformations including a cascade ring-switching process of furostan-26-acid, an epimerization of C25 controlled by the conformation of six-membered lactone ring, an intramolecular Schmidt reaction, and an imine reduction/intramolecular aminolysis process. To address the epimerization issue during Schmidt reaction, an improved synthesis was developed, which also led to a synthesis of 22-epi-solanidine. In this synthesis, selective transformation of azido lactone to azido diol and amino diol was realized through a reduction relay tactic. The azido diol was transformed to solanidine via an intramolecular Schmidt reaction/N-alkylation/reduction process and to 22-epi-solanidine via an intramolecular double N-alkylation process.

Güney-Batı Anadolu Bölgesindeki kızılçam (Pinus brutia Ten. kültür ormanlarında değişik silvikültürel uygulamalara göre artım ve büyüme ilişkileri

Directory of Open Access Journals (Sweden)

Dr. Neşat Erkan

2017-11-01

Full Text Available Bu çalışma ile sıklık çağına gelmiş kızılçam ağaçlandırma alanlarında yapılacak değişik dozdaki ayıklamaların ve aralamaların, meşcere artım ve büyümesi üzerine olan etkilerinin incelenmesi amaçlanmıştır. Araştırma, 1982-2016 yılları arasında Kaş-Tekircik ve Eğirdir-Aşağı Narlı kızılçam ağaçlandırma alanı olmak üzere iki yerde yürütülmüştür. Rastlantı blokları deneme deseni kullanılmış; Kaş ve Eğirdir deneme alanlarında toplam 5 blok ve 35 parselden elde edilen veriler değerlendirilmiştir. Araştırmada aralama şiddeti işlem olarak; 1 kontrol, 2 mutedil aralama ve 3 şiddetli aralama şeklinde üç işlemli bir uygulama yapılmıştır. Deneme parsellerinde, kontrol parselleri hariç olmak üzere, 1990 ve 2004 yıllarında, meşcerenin ihtiyaç gösterdiği parsellerde ve düzeyde aralamalara devam edilmiştir. Araştırma sonucu; Meşcereye müdahale edilen parsellerde, 1985, 1990 ve 2004 yıllarında yapılan ölçme sonuçlarına göre Ortalama çapın, belirgin bir şekilde arttığını göstermiştir. Yine her iki yerde, toplam 5 blok üzerinden yapılan varyans analiz sonucunda, aralamaların göğüs yüzeyi ve kalan meşcere hacmi üzerinde p<0,05 anlamlılık düzeyinde etkili olduğu anlaşılmıştır. Parsellerde 44. yaşında, kalan meşcere hacmi için yapılan varyans analizi sonuçlarına göre, aralama şiddetinin kalan meşcere hacmi üzerinde etkili olduğu gözlenmiştir. Genel meşcere hacminin ise, yapılan istatistik analizler sonucunda p<0,05 anlamlılık düzeyinde, aralamalardan etkilenmediği sonucuna ulaşılmıştır. Sonuç olarak, kızılçam ormanlarında, işletme amaçları çerçevesinde, büyümeyi kaliteli gövdeler üzerinde toplamaya yönelik olarak, büyümenin hızlı olduğu genç yaşlarda, ekonomik açıdan da uygun olacağı düşüncesi ile müdahale sıklığını azaltmak ve bunun için de şiddetli aralama ile göğüs y

Apoptosis induced by (di-isopropyloxyphoryl-Trp) -Lys-OCH in K562 ...

Indian Academy of Sciences (India)

PRAKASH KUMAR G

Kornblau S M 1998 The role of apoptosis in the pathogenesis, prognosis, and therapy of ... Reeves J P 1979 Accumulation of amino acids by lysosomes incubated with amino acid ... of disease; Science 267 1456–1462. Van Engeland M ...

Incretin effect after oral amino Acid ingestion in humans

DEFF Research Database (Denmark)

Lindgren, Ola; Pacini, Giovanni; Tura, Andrea

2015-01-01

is also present after amino acid ingestion is not known. OBJECTIVE: The objective of the study was to explore insulin secretion and incretin hormones after oral and iv amino acid administration at matched total amino acid concentrations in healthy subjects. DESIGN: An amino acid mixture (Vaminolac......) was administered orally or iv at a rate resulting in matching total amino acid concentrations to 12 male volunteers with age 22.5 ± 1.4 years and a body mass index 22.4 ± 1.4 kg/m(2), who had no history of diabetes. MAIN OUTCOME MEASURES: Main outcome measures were area under the 120-minute curve for insulin, C...... after oral than after iv amino acid challenges (P = .006), whereas there was no significant difference in the glucagon response. Intact and total GIP rose after oral but not after iv amino acid administration, whereas intact and total GLP-1 levels did not change significantly in either test. CONCLUSION...

Akademik Başarının Arttırılmasında Aile Katılımı

Directory of Open Access Journals (Sweden)

Bahar Keçeli-Kaysılı

2008-01-01

Full Text Available Çocukların başarılarının arttırılmasını amaçlayan aile katılımı, ailelerin konuya özgü becerileri öğrenmelerinden aile içinde uygun ilişkilerin geliştirilmesine kadar pek çok becerinin kazandırılmasını kapsamaktadır. Aile katılımının her ne kadar yasalarda geçen hükümlerle gerekliliği belirlenmiş olsa da uygulama alanında günümüzde halen pek çok ülke için katılımın sağlanması güç olmaktadır. Alan yazında aile katılımının akademik başarıya etkisine yönelik birbiriden farklı sonuçlarla karşılaşılmıştır. Bunun nedeni aile katılımı kavramının, pek çok değişkeni barındırıyor olması ve bu kavramın farklı şekillerde tanımlanıyor olmasıdır. Her ne kadar aile katılımının hangi bileşeninin akademik başarıya etki ettiği açıkça ortaya konulamasa da araştırma bulguları aile katılımının öğrencilerin akademik becerilerini arttırdığını göstermektedir. Bu alan yazın taramasında aile katılımı ve akademik başarı tanımlanmış, aile katılımını engelleyen etmenler, açıklanmaya çalışılmıştır. Aile katılımının akademik başarıya etkisini gösteren çalışmalara ayrıntılı olarak yer verilmiş, bu çalışmaların bulduğu farklı sonuçlar ve nedenleri tartışılmıştır. Aile katılımının sağlanması, ekolojik bakış açısı ve sistem yaklaşımı bağlamında ele alınarak, okul açısından aile katılımını destekleyici stratejilere değinilmiştir. Parental involvement, which aims to increase the academic achievement of children, encompasses a wide of range of skills from the families’ learning the relevant skills to the development of appropriate relationships within the family. Although the need for parental involvement is stipulated by clauses in various laws, in practice, participation is not fully established yet in many countries. The literature of the field has various findings regarding the impact of

22 CFR 228.22 - Air transportation.

Science.gov (United States)

2010-04-01

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Synthesis of CdS nanoparticles quantum dots capped by 2,2′-dithiodibenzoic acid and study of its interaction with some transition metal ions

International Nuclear Information System (INIS)

Hosseini, Mohammad Saeid; Jahanbani, Hamideh

2013-01-01

In this work, CdS quantum dots (QDs) capped by 2,2′-dithiodibenzoic acid (DDBA) were prepared at one step. The size, shape, components and spectral properties of DDBA-capped-CdS QDs were characterized by transmission electron microscopy, infrared spectrometry and spectroflourometry. The results showed that the prepared QDs with an average diameter of 15 nm have a favorable fluorescence, which is highly affected by pH of the environment. Interaction of some heavy metal ions including Ag(I), Hg(II), Cu(II), Fe(II), Ni(II), pb(II), Mn(II), Zn(II) and Co(II) with DDBA-capped-CdS QDs was investigated at different buffering pH media. Based on the fluorescence quenching of the QDs in the presence of metal ion of interest, feasibility of their determinations were examined according to the Stern–Volmer equation. The investigations showed that Ag(I) ions can be determined in the presence of many co-existence metal ions at the buffering pH of 7. This method was then applied for Ag(I) measurement in some environmental samples, satisfactorily. -- Highlights: ► A new CdS quantum dot capped with 2,2′-dithiodibenzoic acid (DDBA) was prepared. ► The prepared QDs benefit from a favorable fluorescence. ► Interaction of some metal ions with the QDs was examined according to the Stern–Volmer equation. ► The determination of Ag(I) is feasible in the presence of many co-existence metal ions. ► The method benefits from high-speed and considerable simplicity for Ag(I) determination

Profile of Fatty Acids, Amino Acids, Carotenoid Total, and α-Tocopherol from Flying Fish Eggs

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Aulia Azka

2015-12-01

Full Text Available Flying fish are found in waters of eastern Indonesia, which until now is still limited informationabout nutritional content. The purpose of this research was determine the composition offatty acids, amino acids, total carotenoids, α-tocopherol flying fish eggs (Hyrundicthys sp..The composition of fatty acid was measured by gas chromatography (GC, while amino acids,total carotenoids, α-tocopherol was measured by High performanced Liquid Chromatography(HPLC. Egg contained 22 fatty acids such as saturated fatty acid 29.71%, monounsaturated fattyacid 7.86%, and polysaturated fatty acid 13.64%. The result showed that eggs flying fish contained17 amino acids, such as essential amino acid 14.96% and non-essential amino acids 20.27%. Eggscontained a total carotenoid of 245.37 ppm. α-tocopherol content of flying fish eggs by 1.06 ppm.Keywords: Amino acids, carotenoid total, fatty acid, flying fish egg, α-tocopherol

Brachionus plicatilis (Rotifer'in Farklı Besin Ortamlarında Büyümesi.

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Bircan Özbaş

2015-12-01

Full Text Available Farklı besin diyetlerinin rotifer (Brachionus plicatilis kültürlerinin büyümesi üzerindeki etkilerinin araştırıldığı bu çalışmada, rotiferler Nannochloropsis sp., ekmek mayası (Saccharomyces cerevisiae ve kurutulmuş Spirulina tozu ile beslendi. İlk denemede, optimum Spirulina konsantrasyonu günde 0.01 g / 106 rotifer olarak bulundu. Deneme gruplarında alg, alg+maya, alg+Spirulina, maya+Spirulina ve maya/2+Spirulina olmak üzere 5 deneme grubu oluşturuldu. Mikroskobik gözlemlerde, Spirulina partikülleri havanda ezilmiş olmasına rağmen rotiferler tarafından tüketilemeyecek boyutta idi. Sonuç olarak, alg içeren grupların en iyi büyümeyi gösterdiği tespit edildi. Spirulina ise partikül büyüklüğünden dolayı rotiferler tarafından alınamadı. Maya hücreleri ise rotifer hücreleri için optimum büyüklükte olmasına rağmen, ortam kirliliğine neden olması bakımından alg ile beslenen gruplara göre daha düşük bir büyüme gösterdi

Vakumla Paketlenen İnegöl Köftelerin Farklı Derecelerde Buzdolabında Saklanması Sırasında Bakteri Florasında ve Listeria monocytogenes Sayısındaki Değişiklikler

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Ece Soyutemiz

2015-02-01

Full Text Available Çalışma, günümüzde hazır olarak satışı yaygınlaşan inegöl köftenin vakumla paketlenerek farklı derecelerde muhafazası sırasın­da köftenin bakteri florasını ve L. monocytogenes 'ingelişimini incelemek amacıyla yapıldı. Birinci grup köftelere 4.0x103 kob/g, 9.0x103 kob/g ve 2.4x104 kob/g düzeylerinde 3 farklı L. monocytogenes inokülasyonu uygulanarak, va­kumla paketleme işleminden sonra 7 gün süreyle 4°C'de muhafaza edildi, ikinci grup köftelere ise, 2,9x103 kob/g, 7.0x102 kob/g ve 4.3x103 kob/g düzeylerinde etken inoküle edilerek vakumla paketlendi ve 4°C'de 7 gün süreyle muhafaza edildi. Ayrıca köfteler mezofil aerob bakteri, psikrofil bakteriler, koliform bakteriler, E.coli,stafilokok ve mikrokoklar, koagülaz pozitif stafilokoklar, sülfit indirgeyen anaeroblar, laktobasiller, maya-küf ve enterokoklar bakımından analizlere alındı. Köftelerin ayrınca pH değerleri belirtendi. Analizler depolamanın 0, 1, 2, 3, 4, 5, 6 ve 7. günlerinde yapıldı. İnegöl köftelerin, vakumla paketlenerek 0°C'de ve 4°C'de muhafaza işlemi L. monocytogenes 'inçoğalmasını önlese bile etken bu süre içinde varlığını sürdürmeye devam etti. Bu nedenle köfte yapımında hijyen prensiplerinin ve köftelere yeterli ızgara işlemi uygulamasının önem ka­zandığı düşünüldü.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to Rhodiola rosea L. extract and reduction of mental fatigue pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Nutrilinks Sarl, submitted pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to DHA and contribution to normal brain development pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from DSM Nutritional Products, submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of the United Kingdom, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of a health claim related to Slendesta® Potato Extract and reduction of body weight pursuant to Article 13(5) of Regulation (EC) No 19 24/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Kemin Foods LC, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion...

Multidrug resistance in tumour cells: characterisation of the multidrug resistant cell line K562-Lucena 1

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VIVIAN M. RUMJANEK

2001-03-01

Full Text Available Multidrug resistance to chemotherapy is a major obstacle in the treatment of cancer patients. The best characterised mechanism responsible for multidrug resistance involves the expression of the MDR-1 gene product, P-glycoprotein. However, the resistance process is multifactorial. Studies of multidrug resistance mechanisms have relied on the analysis of cancer cell lines that have been selected and present cross-reactivity to a broad range of anticancer agents. This work characterises a multidrug resistant cell line, originally selected for resistance to the Vinca alkaloid vincristine and derived from the human erythroleukaemia cell K562. This cell line, named Lucena 1, overexpresses P-glycoprotein and have its resistance reversed by the chemosensitisers verapamil, trifluoperazine and cyclosporins A, D and G. Furthermore, we demonstrated that methylene blue was capable of partially reversing the resistance in this cell line. On the contrary, the use of 5-fluorouracil increased the resistance of Lucena 1. In addition to chemotherapics, Lucena 1 cells were resistant to ultraviolet A radiation and hydrogen peroxide and failed to mobilise intracellular calcium when thapsigargin was used. Changes in the cytoskeleton of this cell line were also observed.A resistência a múltiplos fármacos é o principal obstáculo no tratamento de pacientes com câncer. O mecanismo responsável pela resistência múltipla mais bem caracterizado envolve a expressão do produto do gene MDR-1, a glicoproteína P. Entretanto, o processo de resistência tem fatores múltiplos. Estudos de mecanismos de resistência m��ltipla a fármacos têm dependido da análise de linhagens celulares tumorais que foram selecionadas e apresentam reatividade cruzada a uma ampla faixa de agentes anti-tumorais. Este trabalho caracteriza uma linhagem celular com múltipla resistência a fármacos, selecionada originalmente pela resistência ao alcalóide de Vinca vincristina e derivado

TÜRK SİNEMASI'NDA İSTANBUL İMGESİ

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ibrahim yenen

2012-09-01

Full Text Available Sinema teknik ve sanatsal bir içeriğe sahip olmakla birlikte toplumsal bir uzanıma da sahiptir. Sinemanın bu sosyal boyutu farklı kültürel öğelerin temsillerini beraberinde getirmektedir. Sosyo-ekonomik ve kültürel hayatın bütün ayrıntılarını içeren toplumsal hayatın farklı görünümleri sinematografik imgelemin nesnesi olabilmektedir. Erkek-kadın ilişkileri, yoksulluk, adalet, eğitim ve din gibi konuların yanında şehirler sinemanın ilgi gösterdiği konuların başında gelmektedir. Soyut kültürel estetiğin fiziksel bir forma dönüştüğü mekânlar olarak şehirler film anlatılarının belirgin veya gizli aktörleri olmuşlardır. Genelde filmsel bir hikâye anlatısının mekânsal arka planını oluşturduğu varsayılan şehirler fon olma özelliğinin ötesinde etkin bir rolle temsil edilebilmişlerdir. Bu anlamda Türk Sinema’sında imgelenen İstanbul, son yüzyılda ülkemizin geçirmekte olduğu sosyal ve kültürel dönüşümün seyrini izleme imkânı sunmaktadır. Anahtar Kelimeler: Sinema, kent, İstanbul, imge Beside the cinema have tecnical and artical context also have a social expension. This cinema’s social dimensions bring about represantation which have different cultural component. Various apperiances of social life that consisting of socio-economic and cultural lifes details may be the object of cinematographic imagery. Like man-women relations, poverty, justice, education and religion also cities is used one of main film topic. The cities which in nonphysical esthetic turn into physical form is distinctive or confidential agency in film scripties. This cities that consist of the back ground of film scripties is represented not only filmic phon also active role. In this sense Istanbul was to imagined in Tukish films offer chance to observe social and culturel transformation that our country encountered in last century. L'image d’Istanbul dans le cinéma turc Le cinéma a des

Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis

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Sun Hee Kim

2015-01-01

Full Text Available Reconstitution of nonnative, very-long-chain polyunsaturated fatty acid (VLC-PUFA biosynthetic pathways in Arabidopsis thaliana was undertaken. The introduction of three primary biosynthetic activities to cells requires the stable coexpression of multiple proteins within the same cell. Herein, we report that C22 VLC-PUFAs were synthesized from C18 precursors by reactions catalyzed by Δ6-desaturase, an ELOVL5-like enzyme involved in VLC-PUFA elongation, and Δ5-desaturase. Coexpression of the corresponding genes (McD6DES, AsELOVL5, and PtD5DES under the control of the seed-specific vicilin promoter resulted in production of docosapentaenoic acid (22:5 n-3 and docosatetraenoic acid (22:4 n-6 as well as eicosapentaenoic acid (20:5 n-3 and arachidonic acid (20:4 n-6 in Arabidopsis seeds. The contributions of the transgenic enzymes and endogenous fatty acid metabolism were determined. Specifically, the reasonable synthesis of omega-3 stearidonic acid (18:4 n-3 could be a useful tool to obtain a sustainable system for the production of omega-3 fatty acids in seeds of a transgenic T3 line 63-1. The results indicated that coexpression of the three proteins was stable. Therefore, this study suggests that metabolic engineering of oilseed crops to produce VLC-PUFAs is feasible.