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Sample records for achromobacter sp ao22

  1. A Tn5051-like mer-containing transposon identified in a heavy metal tolerant strain Achromobacter sp. AO22

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    Bhave Mrinal

    2009-03-01

    Full Text Available Abstract Background Achromobacter sp. AO22 (formerly Alcaligenes sp. AO22, a bacterial strain isolated from a lead-contaminated industrial site in Australia, was previously found to be resistant to moderate to high levels of mercury, copper and other heavy metals. However, the nature and location of the genetic basis for mercuric ion resistance in this strain, had not been previously identified. Findings Achromobacter sp. AO22 contains a functional mer operon with all four essential genes (merRTPA and shows >99% DNA sequence identity to that of Tn501. The mer operon was present on a transposon, designated TnAO22, captured by introducing a broad-host-range IncP plasmid into Achromobacter sp. AO22 and subsequently transferring it to E. coli recipients. The transposition frequency of TnAO22 was 10-2 to 10-3 per target plasmid transferred. Analysis of TnAO22 sequence revealed it belonged to the Tn21 subgroup of the Tn3 superfamily of transposons, with the transposition module having >99% identity with Tn5051 of a Pseudomonas putida strain isolated from a water sample in New York. Conclusion TnAO22 is thus a new variant of Tn5051 of the Tn3 superfamily and the transposon and its associated mercury resistance system are among the few such systems reported in a soil bacterium. Achromobacter sp. AO22 can thus be exploited for applications such as in situ mercury bioremediation of contaminated sites, or the mobile unit and mer operon could be mobilized to other bacteria for similar purposes.

  2. Remediation of PAH-contaminated soil using Achromobacter sp

    International Nuclear Information System (INIS)

    Cutright, T.J.; Lee, S.

    1994-01-01

    Several technologies have the potential to effectively remediate soil contaminated with polycyclic aromatic hydrocarbons (PAHs): solvent extraction, coal-oil agloflotation, supercritical extraction, and bioremediation. Due to the cost effectiveness and in-situ treatment capabilities of bioremediation, studies were conducted to determine the efficiency of Achromobacter sp. to remediate an industrial contaminated soil sample. Specifically, the use of three different mineral salt solutions in conjunction with the Achromobacter sp. was investigated. The molecular identification of the contaminants and their respective levels after remediation were determined using a Hewlett-Packard 1050 HPLC. Preliminary results show a 92% remediation for the use of two of the mineral salt solutions after 20 days' treatment. After 8 weeks, the remediation efficiency reached 99%. Bioremediation was also critically compared to the other potential remediation technologies

  3. Genetic Adaptation of Achromobacter sp. during Persistence in the Lungs of Cystic Fibrosis Patients.

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    Winnie Ridderberg

    Full Text Available Achromobacter species are increasingly isolated from the respiratory tract of cystic fibrosis patients and often a chronic infection is established. How Achromobacter sp. adapts to the human host remains uncharacterised. By comparing longitudinally collected isolates of Achromobacter sp. isolated from five CF patients, we have investigated the within-host evolution of clonal lineages. The majority of identified mutations were isolate-specific suggesting co-evolution of several subpopulations from the original infecting isolate. The largest proportion of mutated genes were involved in the general metabolism of the bacterium, but genes involved in virulence and antimicrobial resistance were also affected. A number of virulence genes required for initiation of acute infection were selected against, e.g. genes of the type I and type III secretion systems and genes related to pilus and flagellum formation or function. Six antimicrobial resistance genes or their regulatory genes were mutated, including large deletions affecting the repressor genes of an RND-family efflux pump and a beta-lactamase. Convergent evolution was observed for five genes that were all implicated in bacterial virulence. Characterisation of genes involved in adaptation of Achromobacter to the human host is required for understanding the pathogen-host interaction and facilitate design of future therapeutic interventions.

  4. Endosulfan induced alteration in bacterial protein profile and RNA yield of Klebsiella sp. M3, Achromobacter sp. M6, and Rhodococcus sp. M2.

    Science.gov (United States)

    Singh, Madhu; Singh, Dileep Kumar

    2014-01-30

    Three bacterial strains identified as Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2 were isolated by soil enrichment with endosulfan followed by shake flask enrichment technique. They were efficiently degrading endosulfan in the NSM (non sulfur medium) broth. Degradation of endosulfan was faster with the cell free extract of bacterial cells grown in the sulfur deficient medium (NSM) supplemented with endosulfan than that of nutrient rich medium (Luria Bertani). In the cell free extract of NSM supplemented with endosulfan as sole sulfur source, a unique band was visualized on SDS-PAGE but not with magnesium sulfate as the sole sulfur source in NSM and LB with endosulfan. Expression of a unique polypeptide band was speculated to be induced by endosulfan under sulfur starved condition. These unique polypeptide bands were identified as OmpK35 protein, sulfate binding protein and outer membrane porin protein, respectively, in Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2. Endosulfan showed dose dependent negative effect on total RNA yield of bacterial strains in nutrient rich medium. Absence of plasmid DNA indicated the presence of endosulfan metabolizing gene on genomic DNA. Copyright © 2013 Elsevier B.V. All rights reserved.

  5. Evidence for cooperative mineralization of diuron by Arthrobacter sp. BS2 and Achromobacter sp. SP1 isolated from a mixed culture enriched from diuron exposed environments.

    Science.gov (United States)

    Devers-Lamrani, Marion; Pesce, Stéphane; Rouard, Nadine; Martin-Laurent, Fabrice

    2014-12-01

    Diuron was found to be mineralized in buffer strip soil (BS) and in the sediments (SED) of the Morcille river in the Beaujolais vineyard repeatedly treated with this herbicide. Enrichment cultures from BS and SED samples led to the isolation of three bacterial strains transforming diuron to 3,4-dichloroaniline (3,4-DCA) its aniline derivative. 16S rRNA sequencing revealed that they belonged to the genus Arthrobacter (99% of similarity to Arthrobacter globiformis strain K01-01) and were designated as Arthrobacter sp. BS1, BS2 and SED1. Diuron-degrading potential characterized by sequencing of the puhA gene, characterizing the diuron-degradaing potential, revealed 99% similarity to A. globiformis strain D47 puhA gene isolated a decade ago in the UK. These isolates were also able to use chlorotoluron for their growth. Although able to degrade linuron and monolinuron to related aniline derivatives they were not growing on them. Enrichment cultures led to the isolation of a strain from the sediments entirely degrading 3,4-DCA. 16S rRNA sequence analysis showed that it was affiliated to the genus Achromobacter (99% of similarity to Achromobacter sp. CH1) and was designated as Achromobacter sp. SP1. The dcaQ gene encoding enzyme responsible for the transformation of 3,4-DCA to chlorocatechol was found in SP1 with 99% similarity to that of Comamonas testosteroni WDL7. This isolate also used for its growth a range of anilines (3-chloro-4-methyl-aniline, 4-isopropylaniline, 4-chloroaniline, 3-chloroaniline, 4-bromoaniline). The mixed culture composed of BS2 and SP1 strains entirely mineralizes (14)C-diuron to (14)CO2. Diuron-mineralization observed in the enrichment culture could result from the metabolic cooperation between these two populations. Copyright © 2014. Published by Elsevier Ltd.

  6. Isolation and characterization of a novel hydrocarbon-degrading bacterium Achromobacter sp. HZ01 from the crude oil-contaminated seawater at the Daya Bay, southern China

    International Nuclear Information System (INIS)

    Deng, Mao-Cheng; Li, Jing; Liang, Fu-Rui; Yi, Meisheng; Xu, Xiao-Ming; Yuan, Jian-Ping; Peng, Juan; Wu, Chou-Fei; Wang, Jiang-Hai

    2014-01-01

    Graphical abstract: Morphological properties of the colonies and cells of strain HZ01. (A) Colonies of strain HZ01 on the LB solid plate; (B) Gram-negative bacterium of strain HZ01 (20 × 100); (C) Scanning electron microscopy (SEM) photograph of strain HZ01 (×15,000); and (D) Transmission electronic microscopy (TEM) photograph of strain HZ01 (×5000). - Highlights: • A novel petroleum degrading bacterium HZ01 was obtained from the crude oil-contaminated seawater. • Strain HZ01 had been identified as Achromobacter sp. • Strain HZ01 could degrade the evaporated diesel oil with the degradability of 96.6%. • Strain HZ01 could effectively degrade anthracene, phenanthrene and pyrence. • Strain HZ01 may be employed to remove hydrocarbon contaminants. - Abstract: Microorganisms play an important role in the biodegradation of petroleum contaminants, which have attracted great concern due to their persistent toxicity and difficult biodegradation. In this paper, a novel hydrocarbon-degrading bacterium HZ01 was isolated from the crude oil-contaminated seawater at the Daya Bay, South China Sea, and identified as Achromobacter sp. Under the conditions of pH 7.0, NaCl 3% (w/v), temperature 28 °C and rotary speed 150 rpm, its degradability of the total n-alkanes reached up to 96.6% after 10 days of incubation for the evaporated diesel oil. Furthermore, Achromobacter sp. HZ01 could effectively utilize polycyclic aromatic hydrocarbons (PAHs) as its sole carbon source, and could remove anthracene, phenanthrene and pyrence about 29.8%, 50.6% and 38.4% respectively after 30 days of incubation. Therefore, Achromobacter sp. HZ01 may employed as an excellent degrader to develop one cost-effective and eco-friendly method for the bioremediation of marine environments polluted by crude oil

  7. Biodegradation of di-n-Butyl Phthalate by Achromobacter sp. Isolated from Rural Domestic Wastewater

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    Decai Jin

    2015-10-01

    Full Text Available A bacterial strain W-1, isolated from rural domestic wastewater, can utilize the environmental hormone di-n-butyl phthalate (DBP as the sole carbon and energy source. The isolated bacterium species was confirmed to belong to the genus Achromobacter based on its 16S rRNA gene sequence. The results of substrate utilization tests showed that the strain W-1 could utilize other common phthalates and phenol. High-performance liquid chromatography analysis revealed that the optimal conditions for DBP degradation were pH 7.0, 35 °C, and an agitation rate of 175 rpm. Under these conditions, 500 mg/L of DBP was completely degraded within 30 h. The effects of heavy metals (50 mg/L Cu2+ and 500 mg/L Pb2+ and surfactants (100 mg/L SDS and 500 mg/L Tween 20 on DBP degradation were investigated. The results demonstrated that Cu2+ and SDS severely inhibited DBP degradation and Pb2+ weakly inhibited DBP degradation, while Tween 20 greatly enhanced DBP degradation. Furthermore, phthalate degradation genes were found to be located on a plasmid present in Achromobacter sp. W-1.

  8. Penicillin G acylase from Achromobacter sp CCM 4824 An efficient biocatalyst for syntheses of beta-lactam antibiotics under conditions employed in large-scale processes

    Czech Academy of Sciences Publication Activity Database

    Bečka, Stanislav; Štěpánek, Václav; Vyasarayani, W. R.; Grulich, Michal; Maršálek, Jaroslav; Plháčková, Kamila; Dobišová, Marie; Marešová, Helena; Plačková, Martina; Valešová, Renata; Palyzová, Andrea; Datla, A.; Ashar, T. K.; Kyslík, Pavel

    2014-01-01

    Roč. 98, č. 3 (2014), s. 1195-1203 ISSN 0175-7598 Institutional support: RVO:61388971 Keywords : Achromobacter sp. * Penicillin G acylase * beta-Lactam antibiotics Subject RIV: EE - Microbiology, Virology Impact factor: 3.337, year: 2014

  9. Conjuntivite por Achromobacter xylosoxidans: relato de caso Conjunctivitis due to Achromobacter xylosoxidans: case report

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    Juliana Faggion Lucatelli

    2009-04-01

    Full Text Available Relatamos um caso de conjuntivite ocasionada por Achromobacter xylosoxidans em paciente imunocompetente usuária de lentes de contato rígidas. A bactéria foi isolada da solução utilizada para a desinfecção das lentes bem como do raspado conjuntival. A. xylosoxidans tem sido descrita em infecções oportunistas em pacientes imunodeprimidos, contudo pode ser confundida com outros bacilos gram-negativos, principalmente Pseudomonas aeruginosa, isoladas de infecções oculares em pacientes imunocompetentes. Devido ao reduzido perfil de sensibilidade aos antimicrobianos demonstrado pelo A. xylosoxidans, torna-se importante a identificação deste agente etiológico em quadros de conjuntivite.We report here a case of conjunctivitis in an immunocompetent patient due to Achromobacter xylosoxidans, which was associated with the use of rigid contact lenses. The bacteria were isolated from the scraped conjunctival swab as well as from the lens cleaning fluid. A. xylosoxidans is an opportunistic pathogen, especially in immunocompromised patients; however, in isolates of ocular infections, from immunocompetent patients, it may be confused with other gram-negative organisms, particularly Pseudomonas aeruginosa. Due to an increased resistance against different antimicrobial agents, A. xylosoxidans must be fully identified and differentiated from other gram-negative isolates from ocular infections.

  10. Achromobacter species in cystic fibrosis

    DEFF Research Database (Denmark)

    Hansen, C R; Pressler, T; Ridderberg, W

    2013-01-01

    Achromobacter species leads to chronic infection in an increasing number of CF patients. We report 2 cases of Achromobacter ruhlandii cross-infection between patients after well-described indirect contact....

  11. A pan-genomic approach to understand the basis of host adaptation in Achromobacter.

    Science.gov (United States)

    Jeukens, J; Freschi, L; Vincent, A T; Emond-Rheault, J G; Kukavica-Ibrulj, I; Charette, S J; Levesque, R C

    2017-04-05

    Over the past decade, there has been a rising interest in Achromobacter sp., an emerging opportunistic pathogen responsible for nosocomial and cystic fibrosis (CF) lung infections. Species of this genus are ubiquitous in the environment, can outcompete resident microbiota, and are resistant to commonly used disinfectants as well as antibiotics. Nevertheless, the Achromobacter genus suffers from difficulties in diagnosis, unresolved taxonomy and limited understanding of how it adapts to the CF lung, not to mention other host environments. The goals of this first genus-wide comparative genomics study were to clarify the taxonomy of this genus and identify genomic features associated with pathogenicity and host adaptation. This was done with a widely applicable approach based on pan-genome analysis. First, using all publicly available genomes, a combination of phylogenetic analysis based on 1,780 conserved genes with average nucleotide identity and accessory genome composition allowed the identification of a largely clinical lineage composed of A. xylosoxidans A insuavis A. dolens and A. ruhlandii. Within this lineage, we identified 35 positively selected genes involved in metabolism, regulation and efflux-mediated antibiotic resistance. Second, resistome analysis showed that this clinical lineage carried additional antibiotic resistance genes compared to other isolates. Finally, we identified putative mobile elements that contribute 53% of the genus's resistome and support horizontal gene transfer between Achromobacter and other ecologically similar genera. This study provides strong phylogenetic and pan-genomic bases to motivate further research on Achromobacter, and contributes to the understanding of opportunistic pathogen evolution. © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  12. Genome Sequencing Reveals the Potential of Achromobacter sp. HZ01 for Bioremediation

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    Yue-Hui Hong

    2017-08-01

    Full Text Available Petroleum pollution is a severe environmental issue. Comprehensively revealing the genetic backgrounds of hydrocarbon-degrading microorganisms contributes to developing effective methods for bioremediation of crude oil-polluted environments. Marine bacterium Achromobacter sp. HZ01 is capable of degrading hydrocarbons and producing biosurfactants. In this study, the draft genome (5.5 Mbp of strain HZ01 has been obtained by Illumina sequencing, containing 5,162 predicted genes. Genome annotation shows that “amino acid metabolism” is the most abundant metabolic pathway. Strain HZ01 is not capable of using some common carbohydrates as the sole carbon sources, which is due to that it contains few genes associated with carbohydrate transport and lacks some important enzymes related to glycometabolism. It contains abundant proteins directly related to petroleum hydrocarbon degradation. AlkB hydroxylase and its homologs were not identified. It harbors a complete enzyme system of terminal oxidation pathway for n-alkane degradation, which may be initiated by cytochrome P450. The enzymes involved in the catechol pathway are relatively complete for the degradation of aromatic compounds. This bacterium lacks several essential enzymes for methane oxidation, and Baeyer-Villiger monooxygenase involved in the subterminal oxidation pathway and cycloalkane degradation was not identified. These results suggest that strain HZ01 degrades n-alkanes via the terminal oxidation pathway, degrades aromatic compounds primarily via the catechol pathway and cannot perform methane oxidation or cycloalkane degradation. Additionally, strain HZ01 possesses abundant genes related to the metabolism of secondary metabolites, including some genes involved in biosurfactant (such as glycolipids and lipopeptides synthesis. The genome analysis also reveals its genetic basis for nitrogen metabolism, antibiotic resistance, regulatory responses to environmental changes, cell motility

  13. Bacteremia caused by Achromobacter species in an immunocompromised host.

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    Kish, M A; Buggy, B P; Forbes, B A

    1984-01-01

    A case of bacteremia caused by Achromobacter species in an immunocompromised patient is described. The patient responded to antibiotic therapy. Detailed antibiotic susceptibility data are presented. PMID:6332118

  14. Achromobacter xylosoxidans bacteriaemia in a patient with ...

    African Journals Online (AJOL)

    A case of bacteriaemia due to Achromobacter xylosoxidans in a 14 year old patient with myocarditis and right lobar pneumonia is described. Six consecutive strains of this organism isolated from blood cultures during primary bacteriaemia and relapses were multi-drug resistant but susceptible to cefotaxime and gentamicin ...

  15. Characterization of two Achromobacter xylosoxidans isolates from patients with pertussis-like symptoms

    Institute of Scientific and Technical Information of China (English)

    Fiorella Orellana-Peralta; Michelle Jacinto; Maria J Pons; Cludia Gomes; Carlos Bada; Isabel Reyes; Juana del Valle Mendoza; Joaquim Ruiz

    2015-01-01

    Objective:To characterize two Achromobacter xylosoxidans recovered from 2 patients diagnosed with pertussis during a Bordetella pertussis surveillance program. Methods:Nasopharyngeal swabs from 2 children under 1 year of age with clinical suspicion of pertussis were analyzed by culture and PCR. Results:Two Achromobacter xylosoxidans A8, closely related to Bordetella spp. were recovered from 2 patients diagnosed of pertussis, both carrying the ptxA gene and IS418 the pertussis toxin encoding gene. Subsequently, antibiotic susceptibility was evaluated by disk-diffusion method and by PCR. Conclusions:Although more detailed studies are needed, the present data highlight the possibility that Achromobacter xylosoxidans, closely related Bordetella pertussis microorganisms and not covered under the vaccine umbrella, might also result in cases of whooping cough. Thereby further surveillance is necessary to determine the extension and relevance of their pathogenic role in order to discriminate their real public health implication.

  16. “Watch Out! Pneumonia Secondary to Achromobacter Denitrificans ...

    African Journals Online (AJOL)

    Pneumonia is the cause of significant morbidity and mortality especially in developing countries. The frequency and importance of emerging new pathogens have significant implications for therapy. We report a case of pneumonia caused by a very rare organism, Achromobacter denitrificans which was treated successfully ...

  17. Increased constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. and a decrease in microflora diversity may be indicators of ventilator-associated pneumonia: a prospective study in the respiratory tracts of neonates.

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    Wei Lu

    Full Text Available Ventilator-associated pneumonia (VAP is a common complication and cause of death in neonates on mechanical ventilation. However, it is difficult to define the causes of VAP. To understand the causes of VAP, we undertook a prospective study based on the diversity of the microflora in VAP. The experimental group consisted of newborns who suffered from respiratory distress syndrome (RDS and VAP, while the control group suffered from RDS without VAP. Sputa were collected within 1, 3, and 5 days of ventilation and were divided into six groups. DNA was extracted from the samples, and the 16S rDNA was PCR amplified, separated using denaturing gradient gel electrophoresis (DGGE, cloned and sequenced. The resulting sequences were compared using BLAST. The DGGE pictures were measured, and the richness, Shannon-Wiener index, and cluster maps were analyzed. No differences were found regarding the constituent ratio of any genus between the Non-VAP and VAP group within 1 day after intubation. After 1 to 3 days, the constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the VAP group were higher than those in the Non-VAP group, and the ratios of Serratia sp. and Achromobacter sp. were lower. After 3 to 5 days, the ratios of Klebsiella sp., Acinetobacter sp., Serratia sp., and Achromobacter sp. were lower than those in the Non-VAP group. The richness and Shannon-Wiener index of the Non-VAP group were higher than those of the VAP group from 1 to 3 days after intubation, while no differences were found within 1 day and from 3 to 5 days. We conclude that during the first three days of intubation, the microflora diversity in the lower respiratory tract was reduced due to VAP, and the greater constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the sputum may be indicators of VAP.

  18. Inflammation in Achromobacter xylosoxidans infected cystic fibrosis patients

    DEFF Research Database (Denmark)

    Hansen, C. R.; Pressler, T.; Nielsen, K. G.

    2010-01-01

    BACKGROUND: Achromobacter xylosoxidans infection may cause conspicuous chronic pulmonary inflammation in cystic fibrosis (CF) patients similar to Pseudomonas aeruginosa and the Burkholderia cepacia complex (Bcc). Evolution in lung function was compared in chronically infected patients. Cytokine...

  19. Achromobactor denitrificans SP1 produces pharmaceutically active 25C prodigiosin upon utilizing hazardous di(2-ethylhexyl)phthalate

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    Achromobacter denitrificans SP1 isolated from soil sludge heavily contaminated with plastic waste produced a novel pharmaceutically-active 25C prodigiosin analog during growth in a simple mineral salt medium supplemented with hazardous di(2-ethylhexyl)phthalate (DEHP) blended PVC plastics (in situ) ...

  20. Genome sequencing reveals the potential of an indigenous arsenotrophic bacterium; Achromobacter sp. KAs 3-5 for sub-surface arsenic mobilization and strategies for bioremediation

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    Balaram Mohapatra

    2017-12-01

    Full Text Available Prevalence of toxic arsenic (As oxyanion species in oligotrophic groundwater of south-east Asiatic regions (India and Bangladesh has threatened the health of millions of people. As-transforming bacteria alter the mobility, speciation and bioavailability of As in the aquifer ecosystem, hence play important roles in the biogeochemical cycling of As. Till date, only 19 cultivable As-transforming bacterial strains have been reported but with no description on their detail genomic and physiological perspective of As homeostasis. In this study, the draft genome (5.7 Mbp of an As-transforming, aromatic hydrocarbon utilizing and iron disproportioning indigenous groundwater bacterium KAs 3-5 has been obtained by Ion-Torrent sequencing revealed 65% genomic GC content, 5100 protein coding genes, and taxonomic affiliation to the members of genus Achromobacter, with >85% of genomic completeness. Phylogenomic signatures like MLST of 10 house-keeping genes, cut-off of <95% of average nucleotide/amino acid identity (ANI/OrthoANI/AAI, <0.99 of tetra-nucleotide correlations, and <70% value of DNA-DNA homology with nearest phylogenetic neighbors exhibited its species distinctiveness among all the described Achromobacter sp. members. Pan-genomic analysis confirmed the strain’s potential to adapt wide array of environmental stresses with a higher abundance of unique genes for metabolism of amino acids, polyketide, xenobiotics, nitroso compounds, aromatic hydrocarbons and most necessarily complete operon cluster for As-resistance/transformation/detoxification, as well as genes for transport, and signal transduction mechanisms. The genome analysis also highlighted its genetic determinants for loss of functions for antibiotic resistance, pathogenicity regulations, and gain of new/acquired functions for Fe-transport, fatty acids uptake-metabolism, motility, heavy metal (Cu-Zn-Co metabolism and several putative/hypothetical proteins owing to its capacity to acquired

  1. Early treatment with inhaled antibiotics postpones next occurrence of Achromobacter in cystic fibrosis

    DEFF Research Database (Denmark)

    Wang, Mikala; Ridderberg, W; Hansen, Christine Rønne

    2013-01-01

    In this nationwide retrospective study, we analysed species distribution, antimicrobial susceptibility and time to next occurrence of Achromobacter in Danish cystic fibrosis (CF) patients from 2000 to 2011....

  2. OXA-258 from Achromobacter ruhlandii: a Species Specific Marker

    OpenAIRE

    Papalia, Mariana Andrea; Almuzara, Marisa; Cejas, Daniela; Traglia, German Matias; Ramirez, Maria Soledad; Galanternik, Laura; Vay, Carlos Alberto; Gutkind, Gabriel Osvaldo; Radice, Marcela Alejandra

    2015-01-01

    A new blaOXA-258 gene is described as species specific taxonomic marker for Achromobacter ruhlandii isolates (all recovered from cystic fibrosis patients). Even if the OXA-258 differs from OXA-114 variants, isolates could be misidentified as A. xiloxosidans by the amplification of an inner fragment from the OXA coding gene. A robust Identification of A. ruhlandii can be achieved by sequencing this single OXA gene as well as a more laborious recently proposed MLST scheme Fil: Papalia, Maria...

  3. THE SAGA OF A MALE FERTILITY PROTEIN (SP22)

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    Toxicologic studies designed to identify chemical-induced alterations in the structure and function of the epididymis, particularly the acquisition of fertility by proximal cauda epididymal sperm, have lead to the discovery of a novel sperm protein (SP22) that is well correlated ...

  4. IDENTIFICATION AND MOLECULAR CLONING OF XENOPUS LAEVIS SP22, A PROTEIN ASSOCIATED WITH FERTILIZATION IN MAMMALS

    Science.gov (United States)

    ABSTRACTSP22 is a protein that has been characterized in rats where it has been related with fertility. SP22 homologues have been studied in mouse and man and a definitive role for the protein has not been assigned yet. By means of a polyclonal IgG to recombinant rat SP22...

  5. Mineralization of a Malaysian crude oil by Pseudomonas sp. and Achromabacter sp. isolated from coastal waters

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, J.; Ahmad, M.F.

    1995-12-31

    Regarded as being a potentially effective tool to combat oil pollution, bioremediation involves mineralization, i.e., the conversion of complex hydrocarbons into harmless CO{sub 2} and water by action of microorganisms. Therefore, in achieving optimum effectiveness from the application of these products on crude oil in local environments, the capability of the bacteria to mineralize hydrocarbons was evaluated. The microbial laboratory testing of mineralization on local oil degraders involved, first, isolation of bacteria found at a port located on the west coast of Peninsular Malaysia. Subsequently, these bacteria were identified by means of Biomereux`s API 20E and 20 NE systems and later screened by their growth on a Malaysian crude oil. Selected strains of Pseudomonas sp. and Achromabacter sp. were then exposed individually to a similar crude oil in a mineralization unit and monitored for 16 days for release of CO{sub 2}. Pseudomonas paucimobilis was found to produce more CO{sub 2} than Achromobacter sp. When tested under similar conditions, mixed populations of these two taxa produced more CO{sub 2} than that produced by any individual strain. Effective bioremediation of local crude in Malaysian waters can therefore be achieved from biochemically developed Pseudomonas sp. strains.

  6. Isolation and characterization of numerous novel phages targeting diverse strains of the ubiquitous and opportunistic pathogen Achromobacter xylosoxidans.

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    Johannes Wittmann

    Full Text Available The clinical relevance of nosocomially acquired infections caused by multi-resistant Achromobacter strains is rapidly increasing. Here, a diverse set of 61 Achromobacter xylosoxidans strains was characterized by MultiLocus Sequence Typing and Phenotype MicroArray technology. The strains were further analyzed in regard to their susceptibility to 35 antibiotics and to 34 different and newly isolated bacteriophages from the environment. A large proportion of strains were resistant against numerous antibiotics such as cephalosporines, aminoglycosides and quinolones, whereas piperacillin-tazobactam, ticarcillin, mezlocillin and imipenem were still inhibitory. We also present the first expanded study on bacteriophages of the genus Achromobacter that has been so far a blank slate with respect to phage research. The phages were isolated mainly from several waste water treatment plants in Germany. Morphological analysis of all of these phages by electron microscopy revealed a broad diversity with different members of the order Caudovirales, including the families Siphoviridae, Myoviridae, and Podoviridae. A broad spectrum of different host ranges could be determined for several phages that lysed up to 24 different and in part highly antibiotic resistant strains. Molecular characterisation by DNA restriction analysis revealed that all phages contain linear double-stranded DNA. Their restriction patterns display distinct differences underlining their broad diversity.

  7. A new Huffmanela species, H. schouteni sp. n. (Nematoda: Trichosomoididae) from flying fishes in Curaçao.

    Science.gov (United States)

    Moravec, F; Campbell, B G

    1991-01-01

    A new nematode species, Huffmanela schouteni sp. n., has been established on the basis of its egg morphology and biological characters (adult nematodes are unknown). The dark-shelled eggs of this histozoic parasite occur in masses in the abdominal cavity, serose covers of internal organs and in the liver of the flying fishes Hirundichthys affinis Günther (type host) and Cypselurus cyanopterus Cuvier et Valenciennes in Curaçao. The eggs of H. schouteni sp. n. differ from those in other congeneric species mainly in the absence of small spines on the surface of the transparent envelope enclosing the egg proper, measurements (size of eggs 0.069-0.075 x 0.027-0.030 mm) and their localization in the host. A key to Huffmanela species based on egg morphology has been provided.

  8. Cuidado ao idoso em Campinas-SP: estudo qualitativo com gestores e representantes políticos

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    Déborah Cristina de Oliveira

    2012-12-01

    Full Text Available O estudo busca identificar, descrever e analisar as práticas pertinentes à saúde do idoso na cidade de Campinas-SP, na perspectiva de representantes da saúde do idoso, tendo como base a Política Nacional de Saúde à Pessoa Idosa. Estudo qualitativo, com análise temática de conteúdo, na perspectiva de sujeitos que assumem funções estratégicas na condução da política direcionada ao idoso no município. A partir dos depoimentos dos sujeitos emergiram cinco temas centrais: tempos e lugares; condições de acesso; demandas da população; recursos humanos; promoção à saúde e prevenção de doenças e agravos; gestão. As práticas voltadas à saúde do idoso em Campinas estão em desenvolvimento e precisam ser avaliadas, ampliadas e sistematizadas, tanto nas questões estruturais de recursos humanos, financiamento e gestão, quanto na atenção direta ao idoso.

  9. Efektivitas Pemberian Bacillus sp. D2.2 pada Media Teknis Molase terhadap Kualitas Air dan Performa Pertumbuhan Udang Vaname (Litopenaeus vannamei

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    Ayu NOVITASARI

    2017-07-01

    Full Text Available Vanamei prawns have fast growth and can reared in high density. it has an impact on water quality deterioration and disruption of survival rates and growth. Various ways to cope has been done, one of them is with probiotic bacteria. The new strain of D2.2 bacteria is thought to be effective of ammonia utilization. Probiotics with local bacteria Bacillus sp. D2.2 is cultured on molasses technical medium to be applied semi-mass. The purpose of this study is to asses the efectivity of Bacillus sp. D2.2 inthe molasses technical medium on water quality and growth performance of vaname prawns (Litopenaeus vannamei. The research was used complete randomized design (RAL with four treatments, A (Control, B (Application of 5 ppm Bacillus sp. D2.2 cultured in molasses technical medium, C (Application of 10 ppm Bacillus sp. D2.2 cultured in molasses technical medium, D (Application of 15 ppm Bacillus sp. D2.2 cultured in molasses technical medium were repeated three times each. The results showed no effect on water quality and shrimp survival rate, but absolute growth (W, daily growth rate (GR and feed conversion ratio (FCR showed that B and C treatment had better  than control. Keywords: Vaname shrimp, growth, Bacillus sp. D2.2, molasses technical medium

  10. Purification and synergistic antibacterial activity of arginine derived cyclic dipeptides, from Achromobacter sp. associated with a rhabditid entomopathogenic nematode against major clinically relevant biofilm forming wound bacteria

    Directory of Open Access Journals (Sweden)

    NISHANTH KUMAR S

    2015-08-01

    Full Text Available Skin and chronic wound infections caused by various pathogenic bacteria are an increasing and urgent health problem worldwide. In the present study ethyl acetate cell-free culture filtrate of an Achromobacter sp. associated with a Rhabditis entomopathogenic nematode (EPN, displayed promising antibacterial property and was further purified by silica gel column chromatography to get three different cyclic dipeptides (CDPs. Based on the spectral data and Marfey’s analyses, the CDPs were identified as cyclo(D-Leu-D-Arg (1, cyclo(L-Trp-L-Arg (2, and cyclo(D-Trp-D-Arg (3, respectively. Three CDPs were active against all the ten wound associated bacteria tested. The significant antibacterial activity was recorded by CDP 3, and highest activity of 0.5 µg/ml was recorded against Staphylococcus aureus and Pseudomonas aeruginosa. The synergistic antibacterial activities of CDPs and ampicillin were assessed using the checkerboard microdilution method. The results of the current study recorded that the combined effects of CDPs and ampicillin principally recorded synergistic activity. Interestingly, the combination of CDPs and ampicillin also recorded enhanced inhibited biofilm formation by bacteria. Moreover, CDPs significantly stimulate the production of the anti-inflammatory cytokines IL-10 and IL-4 by human peripheral blood mononuclear cells but are without significant effect on the production of TNF-α, a pro-inflammatory cytokines. The three CDPs have been examined for their activities against intracellular S. aureus in murine macrophages (J774 using 24 h exposure to 1X and 2X MIC concentrations. Significance decrease in intracellular S. aureus burden was recorded by CDPs. CDPs also recorded no cytotoxicity towards FS normal fibroblast, VERO and L231 normal lung epithelial cell lines. Antimicrobial activity of the arginine containing CDPs against the wound associated bacteria is reported here for the first. Moreover, this is also the report on the

  11. Adhesion of Stenotrophomonas maltophilia, Delftia acidovorans, and Achromobacter xylosoxidans to Contact Lenses.

    Science.gov (United States)

    Vijay, Ajay Kumar; Willcox, Mark D P

    2017-09-26

    Contact lens cases become contaminated with microbes during use. We wished to compare the adhesion of uncommon bacterial contaminants isolated from lens cases to contact lenses with and without organic soil. Strains of Delftia acidovorans (001), Stenotrophomonas maltophilia (002 and 006), and Achromobacter xylosoxidans (001) isolated from contact lens cases (test strains) and Pseudomonas aeruginosa (Paer1) isolated from eyes at the time of infiltrative response (control strain) were used. Bacteria were grown and resuspended in phosphate-buffered saline (PBS) or 10% organic soil (heat-killed Saccharomyces cerevisiae resuspended in complement inactivated bovine serum). Two silicone hydrogel (senofilcon A and comfilcon A) and one hydrogel lens (etafilcon A) lens materials were used. Bacteria (1.0×10 and 1.0×10 colony-forming units/mL; CFU/mL) adhered to lenses for 24 hr and the numbers of bacteria adherent to each lens type (with and without organic soil) were estimated by culture. All the four test strains adhered in significantly greater numbers to contact lenses after incubation in inoculum prepared with organic soil compared with PBS-D. acidovorans 001 (0.7 log10 CFU; P0.05). Achromobacter xylosoxidans 001 (PBacteria that are commonly found in contact lens cases adhered to contact lenses in relatively high numbers in the presence of organic soil. This might indicate that a similar phenomenon occurs in the presence of tears. This may facilitate their transfer from the lens to the cornea and the production of corneal infiltrates.

  12. ANALYSIS OR THE POTENTIAL SPERM BIOMARKER, SP22, IN HUMAN SEMEN

    Science.gov (United States)

    ANALYSIS OF THE POTENTIAL SPERM BIOMARKER SP22 IN HUMAN SEMEN Rebecca A. Morris Ph.D.1, Gary R. Klinefelter Ph.D.1, Naomi L. Roberts 1, Juan D. Suarez 1, Lillian F. Strader 1, Susan C. Jeffay 1 and Sally D. Perreault Ph.D.1 1 U.S. EPA / ORD / National Health a...

  13. Bacterial community composition characterization of a lead-contaminated Microcoleus sp. consortium.

    Science.gov (United States)

    Giloteaux, Ludovic; Solé, Antoni; Esteve, Isabel; Duran, Robert

    2011-08-01

    A Microcoleus sp. consortium, obtained from the Ebro delta microbial mat, was maintained under different conditions including uncontaminated, lead-contaminated, and acidic conditions. Terminal restriction fragment length polymorphism and 16S rRNA gene library analyses were performed in order to determine the effect of lead and culture conditions on the Microcoleus sp. consortium. The bacterial composition inside the consortium revealed low diversity and the presence of specific terminal-restriction fragments under lead conditions. 16S rRNA gene library analyses showed that members of the consortium were affiliated to the Alpha, Beta, and Gammaproteobacteria and Cyanobacteria. Sequences closely related to Achromobacter spp., Alcaligenes faecalis, and Thiobacillus species were exclusively found under lead conditions while sequences related to Geitlerinema sp., a cyanobacterium belonging to the Oscillatoriales, were not found in presence of lead. This result showed a strong lead selection of the bacterial members present in the Microcoleus sp. consortium. Several of the 16S rRNA sequences were affiliated to nitrogen-fixing microorganisms including members of the Rhizobiaceae and the Sphingomonadaceae. Additionally, confocal laser scanning microscopy and scanning and transmission electron microscopy showed that under lead-contaminated condition Microcoleus sp. cells were grouped and the number of electrodense intracytoplasmic inclusions was increased.

  14. Fatores associados à submissão ao teste rápido anti-HIV na assistência ao parto

    Directory of Open Access Journals (Sweden)

    Maria Inês Couto de Oliveira

    Full Text Available Resumo Os testes rápidos anti-HIV vêm sendo empregados nas maternidades com vistas à prevenção da transmissão vertical. O objetivo do estudo foi analisar os fatores associados à submissão ao teste rápido anti-HIV (desfecho. Estudo transversal, conduzido em 2009, em 15 hospitais do SUS do Rio de Janeiro/RJ, mediante entrevista a amostra representativa de 835 parturientes internadas e consulta a prontuários. Razões de prevalência ajustadas foram obtidas por regressão de Poisson, segundo modelo hierarquizado, permanecendo no modelo final as variáveis associadas ao desfecho (p ≤ 0,05. Segundo os prontuários (SP, 79,6% das mães foram submetidas ao teste rápido anti-HIV e, segundo as entrevistas (SE, 55,7%. No nível distal, a ausência de companheiro (SP, ter ≥ 6 moradores na residência (SP e a cor da pele não branca (SE se associaram a uma maior prevalência do desfecho. No nível intermediário, não dispor de sorologia negativa para o HIV do pré-natal (SP e SE se associou a uma maior prevalência do desfecho, bem como a realização de pré-natal na rede básica (SP e a não realização de pré-natal (SE. No nível proximal, o parto em hospital não certificado como amigo da criança se associou a uma maior prevalência do desfecho (SP e SE.

  15. Fourier transform infrared spectroscopy of dental unit water line biofilm bacteria

    OpenAIRE

    Liaqat, Iram

    2009-01-01

    Fourier transform-infrared (FT-IR) spectroscopy has become an important tool for rapid analysis of complex biological samples. The infrared absorbance spectrum could be regarded as a “fingerprint” which is a feature of biochemical substances. The FT-IR spectra of fresh and stored dried samples of six bacterial isolates (Klebsiella sp., Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Achromobacter xylosoxidans and Achromobacter sp.) were observed by variation in sample preparation....

  16. Reação de linhagens de feijoeiro ao fungo Fusarium oxysporum f. sp. phaseoli em condições controladas Reaction of common bean lines to Fusarium oxysporum f. sp. phaseoli in controlled conditions

    Directory of Open Access Journals (Sweden)

    Mônica Juliani Zavaglia Pereira

    2011-10-01

    Full Text Available Entre os patógenos que mais contribuem para a redução da produtividade do feijoeiro no Brasil está o Fusarium oxysporum f. sp. phaseoli. O emprego de cultivares resistentes é o controle mais eficaz para esse patógeno. Conduziu-se este trabalho, com o objetivo de avaliar linhagens de feijoeiro (Phaseolus vulgaris L. do banco de germoplasma da Universidade Federal de Lavras (UFLA, quanto a reação ao Fusarium oxysporum f. sp. phaseoli e, ao mesmo tempo, estimar os parâmetros genéticos e fenotípicos que possam auxiliar em futuros programas de melhoramento para esse caráter. Foram avaliadas 367 linhagens em dez experimentos. As testemunhas 'Carioca' (suscetível e 'Carioca MG' (resistente foram utilizadas em todos os experimentos. O delineamento utilizado foi inteiramente ao acaso, com cinco repetições e parcelas de uma planta por vaso. As inoculações foram realizadas segundo a metodologia de corte e imersão de raízes na suspensão de esporos do fungo e as avaliações realizadas aos 21 dias após a inoculação com base no índice de severidade da doença empregando-se notas de 1 (plantas sem sintomas a 9 (plantas mortas.Das linhagens do banco de germoplasma da Universidade Federal de Lavras (UFLA avaliadas, 36,5% foram resistentes ao Fusarium oxysporum f. sp. phaseoli. Entre as resistentes, a maioria foi linhagens obtidas antes de 1990. Das 18 linhagens dos experimentos de VCU, do período de 2005/06, apenas quatro foram suscetíveis. A estimativa da herdabilidade (h² foi elevada (h² = 87%, indicando que, a princípio, o caráter é de fácil seleção.Among the pathogens that most contribute for reducing the productivity of beans in Brazil is the Fusarium oxysporum f. sp. phaseoli. The use of resistant cultivars is the most effective control for this pathogen. The aim of this study was to evaluate germoplasm bean (Phaseolus vulgaris L. lines of the Federal University of Lavras (UFLA as the reaction to the Fusarium oxysporum f

  17. Resolution of concomitant Achromobacter xylosoxidans burn wound infection without adjustment of antimicrobial therapy

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    Zhi Yang Ng

    2014-01-01

    Full Text Available Achromobacter xylosoxidans is part of an emerging group of Gram negative bacterial infections with potentially severe sequelae, especially in the immunocompromised population such as burn patients. While antimicrobial therapy for patients with A. xylosoxidans bacteremia has been reported, the literature is scarce with regard to treatment in patients with positive tissue cultures only. Herein, we report our institution′s experience with such a case and a brief review of the current literature on this micro-organism in the setting of non-bacteremic infection.

  18. Susceptibility to antibiotics of Vibrio sp. AO1 growing in pure culture or in association with its hydroid host Aglaophenia octodonta (Cnidaria, Hydrozoa).

    Science.gov (United States)

    Stabili, Loredana; Gravili, Cinzia; Boero, Ferdinando; Tredici, Salvatore M; Alifano, Pietro

    2010-04-01

    Vibrio harveyi is the major causal organism of vibriosis, causing potential devastation to diverse ranges of marine invertebrates over a wide geographical area. These microorganisms, however, are phenotypically diverse, and many of the isolates are also resistant to multiple antibiotics. In a previous study, we described a previously unknown association between Vibrio sp. AO1, a luminous bacterium related to the species V. harveyi, and the benthic hydrozoan Aglaophenia octodonta. In this study, we analyzed the susceptibility to antibiotics (ampicillin, streptomycin, tetracycline, or co-trimoxazole = mix of sulfamethoxazole and trimetoprim) of Vibrio sp. AO1 growing in pure culture or in association with its hydroid host by using microcosm experiments. The results of minimum inhibitory concentration (MIC) experiments demonstrated that Vibrio sp. AO1 was highly resistant to ampicillin and streptomycin in pure culture. Nevertheless, these antibiotics, when used at sub-MIC values, significantly reduced the hydroid fluorescence. Co-trimoxazole showed the highest inhibitory effect on fluorescence of A. octodonta. However, in all treatments, the fluorescence was reduced after 48 h, but never disappeared completely around the folds along the hydrocaulus and at the base of the hydrothecae of A. octodonta when the antibiotic was used at concentration completely inhibiting growth in vitro. The apparent discrepancy between the MIC data and the fluorescence patterns may be due to either heterogeneity of the bacterial population in terms of antibiotic susceptibility or specific chemical-physical conditions of the hydroid microenvironment that may decrease the antibiotic susceptibility of the whole population. The latter hypothesis is supported by scanning electron microscope evidence for development of bacterial biofilm on the hydroid surface. On the basis of the results obtained, we infer that A. octodonta might behave as a reservoir of antibiotic multiresistant bacteria

  19. Characterization of pbt genes conferring increased Pb(2+) and Cd(2+) tolerance upon Achromobacter xylosoxidans A8

    Czech Academy of Sciences Publication Activity Database

    Hložková, K.; Šuman, J.; Strnad, Hynek; Ruml, T.; Pačes, Václav; Kotrba, P.

    2013-01-01

    Roč. 164, č. 10 (2013), s. 1009-1018 ISSN 1769-7123 R&D Projects: GA MŠk(CZ) 1M0520 Grant - others:GA ČR(CZ) GAP504/11/0484 Program:GA Institutional support: RVO:68378050 Keywords : Achromobacter xylosoxidans * metal tolerance * lead * cadmium * zinc * P1-type ATPase * MerR family * transcriptional control Subject RIV: EB - Genetics ; Molecular Biology

  20. Studies on the occurrence and distribution of heavy metals in ...

    African Journals Online (AJOL)

    The bacteria isolated and identified were. Bacillus subtilis, Bacillus licheniformis, Arthrobacter sp and Achromobacter sp. Fungi isolated were Saccharomyces cerevisiae, Candida utilissima and Sporobolomyces sp. The values for heavy metals for all zones are of public health significance and pose a threat to the survival of ...

  1. UTILIZAÇÃO DE ADESIVO PVA EM COMPENSADOS DE Pinus sp. E Eucalyptus sp. / UTILIZATION OF PVA ADHESIVES IN Pinus sp. and Eucalyptus sp. PLYWOOD

    Directory of Open Access Journals (Sweden)

    C. I. De Campos

    2014-12-01

    Full Text Available Estudos sobre novos adesivos ou resinas para colagem de madeira e derivados estão sendo realizados com a intenção de diminuir o impacto ambiental sem alterar suas propriedades. Por este motivo, novas formulações de adesivos PVA (Acetato de Polivinila vêm sendo desenvolvidas. Este trabalho testou a utilização deste adesivo na produção de compensado de Eucalyptus sp. e Pinus sp., com tempo e temperatura de prensagem, respectivamente, de 10 minutos e90°C. Foram realizados os ensaios físicos de densidade, teor de umidade e absorção, e os ensaios mecânicos de flexão estática e de resistência ao cisalhamento na linha de cola, todos de acordo com as normas ABNT. Obtiveram-se bons resultados com relação à resistência ao cisalhamento na linha de cola, a qual foi superior para os adesivos PVA, se comparados com compensados produzidos com ureia-formaldeído e o fenol-formaldeído, enquanto que os resultados de MOE e MOR apresentaram-se inferiores em ambos os casos. 

  2. Resonance Raman spectra of the copper-sulfur chromophores in Achromobacter cycloclastes nitrite reductase.

    Science.gov (United States)

    Dooley, D M; Moog, R S; Liu, M Y; Payne, W J; LeGall, J

    1988-10-15

    Resonance Raman spectroscopy at ambient temperature and 77 K has been used to probe the structures of the copper sites in Achromobacter cycloclastes nitrite reductase. This enzyme contains three copper ions per protein molecule and has two principal electronic absorption bands with lambda max values of 458 and 585 nm. Comparisons between the resonance Raman spectra of nitrite reductase and blue copper proteins establish that both the 458 and 585 nm bands are associated with Cu(II)-S(Cys) chromophores. A histidine ligand probably is also present. Different sets of vibrational frequencies are observed with 457.9 nm (ambient) or 476.1 nm (77 K) excitation as compared with 590 nm (ambient) or 593 nm (77 K) excitation. Excitation profiles indicate that the 458 and 585 nm absorption bands are associated with separate [Cu(II)-S(Cys)N(His)] sites or with inequivalent and uncoupled cysteine ligands in the same site. The former possibility is considered to be more likely.

  3. Achromobacter xylosoxidans infection in an adult cystic fibrosis unit in Madrid.

    Science.gov (United States)

    Llorca Otero, Laura; Girón Moreno, Rosa; Buendía Moreno, Buenaventura; Valenzuela, Claudia; Guiu Martínez, Alba; Alarcón Cavero, Teresa

    2016-03-01

    Achromobacter xylosoxidans is an emerging pathogen in cystic fibrosis (CF). Although the rate of colonization by this microorganism is variable, prevalence is increasing in CF units. A microbiological/clinical study was conducted on of adult CF patients harboring A. xylosoxidans. Identification and susceptibility testing were performed using MicroScan (Siemens). Decline in lung function was assessed using the variable, annual percentage loss of FEV1 (forced expiratory volume in 1s). A. xylosoxidans was isolated in 18 (19.8%) of 91 patients over a 14-year period. Mean age was 26.6 years (18-39 years). Nine patients (9.8%) were chronically colonized. Piperacillin/tazobactam and imipenem were the most active antibiotics. Mean annual decline in lung function in chronically colonized patients was 2.49%. A. xylosoxidans is a major pathogen in CF. A decreased lung function was observed among patients who were chronically colonized by A. xylosoxidans. Antibiotic therapy should be started early in order to prevent chronic colonization by this microorganism. Copyright © 2015 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

  4. Prevalência de enteroparasitas na população urbana do 2.° subdistrito de Botucatu, SP (Brasil Prevalence of intestinal parasites on the populations of Botucatu, SP (Brazil

    Directory of Open Access Journals (Sweden)

    Mauro R. de Oliveira

    1974-06-01

    Full Text Available Procurou-se conhecer a prevalência de enteroparasitoses na população urbana do 2.° subdistrito de Botucatu, SP (Brasil através de exames coprológicos realizados pelos métodos de FAUST, HOFFMAN e processo de tamização. A prevalência de enteroparasitoses foi relacionada com atributos da população, tais como sexo, idade, cor e com fatores ligados ao meio ambiente. O processo de amostragem empregado foi o casual simples em duplo estágio, sendo o quarteirão a unidade primária do primeiro estágio e o domicílio a unidade do segundo estágio. Os resultados mostraram que 53,76% das 895 pessoas amostradas apresentavam-se infestadas por uma ou mais espécies de parasitas intestinais. As prevalências foram as seguintes: Ancylostomidae, 17,54%; T. trichiurus, 13,63%; A. lumbricoides, 10,69%; S. stercoralis, 6,03%; E. vermicularis, 3,69%; H. nana, 1,79%; Taenia sp, 1,22%; S. mansoni, 0,22%; E. coli, 15,53%; G. lamblia, 14,07%; E. nana, 2,35%; I. bütschlii, 1,01% e E. histolytica, 0,22%.A study of the prevalence of intestinal parasitosis on the population of Botucatu's 2nd Subdistrict, S. Paulo, Brazil, is presented. Passed stool was examined by using FAUST, HOFFMAN and tamization techniques. The prevalence of intestinal parasites is related to population characteristics such as sex, age, race and some environmental features. Simple random sampling technique in double stage was applied. Among 895 examined persons, 53,76% were infected by intestinal parasites. The prevalence of the various parasites were: Ancylostomydae, 17,54%; T. trichiurus, 13,63%; A. lumbricoides, 10,39%; E. vermiculares, 3,69%; H. nana, 1,79%; Taenia sp, 1,22%; E. coli, 15,53%; G. lamblia, 14,07%; E. nana, 2,35%; I. bütschlii, 1,01% and E. histolytica, 0,22%.

  5. Espectroscopia VIS-NIR-SWIR na avaliação de solos ao longo de uma topossequência em Piracicaba (SP

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    José A. M. Demattê

    Full Text Available RESUMOObjetivou-se neste trabalho caracterizar diferentes solos por espectrorradiometria de reflectância ao longo de uma topossequência na região de Piracicaba, SP. Amostras de solo foram coletadas e analisadas em campo, em laboratório de análises químicas e por sensores Vis-NIR (400-2500 nm. Alterações nos solos da topossequência foram identificáveis nas informações espectrais. Constituintes dos solos, tais como, matéria orgânica, mineralogia, formas de óxidos de ferro e granulometria foram determinantes nas variações das feições de absorção e intensidades de reflectância. Cada perfil mostrou características espectrais diferenciadoras entre horizontes, relacionadas à intensidade, feições de absorção e morfologia da curva. A avaliação morfológica não pode ser avaliada pelo sensor, sendo uma de suas limitações. Existe relação entre grau de intemperismo (índices ki, relação silte/argila e mineralogia e dados espectrais. Isso foi observado nos solos originados de basalto, onde houve aumento do ferro extraído pelo ditionito (cristalino e amorfo na sequência Nitossolo Vermelho Latossólico (NVL em direção ao Cambissolo (C e, aumento do ferro amorfo nesta mesma sequência. Na avaliação da topossequência completa observou-se a sequência de absorção centrada em 500 e 850 nm decrescente do Nitossolo Vermelho Latossólico em direção ao Chernossolo, ou seja, na sequência de decréscimo dos teores de ferro cristalino (hematita e goethita e aumento de ferro amorfo, corroborado pelo aumento dos valores do índice ki. Houve relação entre os dados espectrais, o índice ki e a posição do solo na paisagem. Esses resultados mostram que a espectrorradiometria é uma ferramenta promissora para auxiliar o levantamento de solos. Entretanto, há necessidade do suporte à implantação de bibliotecas de dados espectrais de solos com acesso irrestrito aos usuários.

  6. Seroprevalencia de Leptospira sp., Rickettsia sp. Ehrlichia sp. en trabajadores rurales del departamento de Sucre, Colombia Seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in rural workers of Sucre, Colombia

    Directory of Open Access Journals (Sweden)

    Rodrigo Ríos

    2008-06-01

    Full Text Available Objective. Determinar la seroprevalencia de Leptospira sp., Rickettsia sp. y Ehrlichia sp. en trabajadores de áreas rurales del departamento de Sucre. Material y métodos. Se realizó un estudio escriptivo, prospectivo, de corte transversal, que pretendió determinar la seroprevalencia e Leptospira sp., Rickettsia sp. y Ehrlichia sp. en 90 trabajadores de áreas rurales del departamento de Sucre. Se estableció la presencia de anticuerpos séricos anti-IgM específicos anti-Leptospira por la técnica de ELISA indirecta. Para la determinación de Rickettsia sp. y Ehrlichia sp. se uso la técnica de inmunofluorescencia indirecta. Resultados. La población evaluada estaba compuesta por 27 (30% ordeñadores, 21 (23% jornaleros, 18 (20% profesionales del campo y 24 (27% que realizaban otras actividades. Ventidós (24% muestras resultaron positivas en alguna de las pruebas. De éstas, 12 (13,3% fueron positivas para Leptospira sp., 7 (7,8% para Rickettsia sp. y 3 (3,3% ara Ehrlichia sp. Conclusión. Este fue el primer estudio que se llevó a cabo en el departamento de Sucre y permitió demostrar que existe una prevalencia importante de Leptospira p.,Rickettsia sp. y Ehrlichia sp.. Los factores de riesgo ocupacional fueron factores determinantes en la seropositividad.Objective. To determine the seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in agricultural workers of Sucre. Methods. A descriptive prospective cross-sectional study was conducted in ninety rural workers of Sucre. Presence of serum antibodies anti-IgM specific anti-Leptospira by indirect ELISA was established. For the determination of Rickettsia and Ehrlichia indirect inmunoflorescence was used. Results.The population was composed by 27 (30% milkers, 21 (23% day workers, 18 farm professionals (20% and 24 (26% workers in others activities. A total of 22 (24% samples were positive to some test. Twelve (13.3% were positive to Leptospira sp., seven (7.8% to Rickettsia sp

  7. Microbial diversity in firework chemical exposed soil and water samples collected in Virudhunagar district, Tamil Nadu, India.

    Science.gov (United States)

    Dhasarathan, P; Theriappan, P; Ashokraja, C

    2010-03-01

    Microbial diversity of soil and water samples collected from pyrochemicals exposed areas of Virdhunagar district (Tamil Nadu, India) was studied. Soil and water samples from cultivable area, waste land and city area of the same region were also studied for a comparative acount. There is a remarkable reduction in total heterotrophic bacterial population (THB) in pyrochemicals exposed soil and water samples (42 × 10(4) CFU/g and 5.6 × 10(4) CFU/ml respectively), compared to the THB of cultivable area soil and water samples (98 × 10(7) CFU/g and 38.6 × 10(7) CFU/ml). The generic composition the THB of the pyrochemicals exposed samples too exhibited considerable change compared to other samples. Pseudomonas sp. was the predominant one (41.6%) followed by Achromobacter sp. (25%) in pyrochemical exposed soil and Pseudomonas sp. was the predominant one (25%) in pyrochemical exposed water samples followed by Bacillus sp. (25%) and Micrococcus sp. (16.6%). It was observed that Cornybacterium sp. and Micrococcus sp. were absent completely in pyrochemical exposed soil and Achromobacter sp. was missing in the pyrochemical exposed water samples, which were present in the other samples. The outcome of this study clearly demonstrates that pollutants such as chemicals used in pyrotechniques affect the microbial biodiversity and suitable measures have to be taken to control the pollution level and to save biodiversity.

  8. Porta-enxertos para o cultivar Máximo IAC 138-22 de uvas de vinho em Monte Alegre do Sul, SP Rootstocks for wine grape cultivar IAC 138-22

    OpenAIRE

    Maurilo Monteiro Terra; Erasmo José Paioli Pires; Sônia Maria Bonilha Marcondes Coelho; Ilene Ribeiro da Silva Passos; Rui Ribeiro dos Santos; Celso Valdevino Pommer; André Camargo Pereira da Silva; Ivan José Antunes Ribeiro

    1990-01-01

    Em Monte Alegre do Sul, SP, efetuou-se um experimento de longa duração para observar o comportamento do cultivar IAC 138-22 (Máximo) de uvas para vinho, sobre cinco porta-enxertos: RR 101-14, 'Golia', "Ripária do Traviú', IAC 572 e IAC 313. Em oito colheitas sucessivas anuais, de 1974 a 1981, analisaram-se as produções médias de uvas por planta. Os resultados indicaram que o IAC 572' foi o melhor porta-enxerto para o IAC 138-22 'Máximo'. O IAC 313 superou o IAC 572 em um dos anos estudados. T...

  9. Bifidobacterium reuteri sp. nov., Bifidobacterium callitrichos sp. nov., Bifidobacterium saguini sp. nov., Bifidobacterium stellenboschense sp. nov. and Bifidobacterium biavatii sp. nov. isolated from faeces of common marmoset (Callithrix jacchus) and red-handed tamarin (Saguinus midas).

    Science.gov (United States)

    Endo, Akihito; Futagawa-Endo, Yuka; Schumann, Peter; Pukall, Rüdiger; Dicks, Leon M T

    2012-03-01

    Five strains of bifidobacteria were isolated from faeces of a common marmoset (Callithrix jacchus) and a red-handed tamarin (Saguinus midas). The five isolates clustered inside the phylogenetic group of the genus Bifidobacterium but did not show high sequence similarities between the isolates and to known species in the genus by phylogenetic analysis based on 16S rRNA gene sequences. Sequence analyses of dnaJ1 and hsp60 also indicated their independent phylogenetic positions to each other in the Bifidobacterium cluster. DNA G+C contents of the species ranged from 57.3 to 66.3 mol%, which is within the values recorded for Bifidobacterium species. All isolates showed fructose-6-phosphate phosphoketolase activity. Based on the data provided, the five isolates represent five novel species, for which the names Bifidobacterium reuteri sp. nov. (type strain: AFB22-1(T) = JCM 17295(T) = DSM 23975(T)), Bifidobacterium callitrichos sp. nov. (type strain: AFB22-5(T) = JCM 17296(T) = DSM 23973(T)), Bifidobacterium saguini sp. nov. (type strain: AFB23-1(T) = JCM 17297(T) = DSM 23967(T)), Bifidobacterium stellenboschense sp. nov. (type strain: AFB23-3(T) = JCM 17298(T) = DSM 23968(T)) and Bifidobacterium biavatii sp. nov. (type strain: AFB23-4(T) = JCM 17299(T) = DSM 23969(T)) are proposed. Copyright © 2011 Elsevier GmbH. All rights reserved.

  10. Preservacão de micélio de Batkoa sp. E. Furia sp. (Entomophthorales) em combinaçao com dessecantes e rudutores de oxigênio

    OpenAIRE

    Leite, Luis G.; Alves, Sérgio B.; Filho, Antonio Batista; Almeida, J. E. M.; Roberts, Donald W.

    2002-01-01

    PRESERVATION OF BATKOA SP. AND FURIA SP. (ENTOMOPHTHORALES )DRY MYCELIUM WITH COMBINATIONS OF DESICCANTS AND OXYGEN REDUCERS. Formulation is the most difficult part of Entomophthorales development as biopesticides and bioacaricides, and has caused difficulty in evaluating the Batkoa sp. and Furia sp. for the control of the cercopids Mahanarva fimbriolata and Deois schach, pests of sugar-cane and pastures. A technique for producing dry mycelium of Entomophthorales was previously developed to a...

  11. Isolate-specific effects of patulin, penicillic Acid and EDTA on biofilm formation and growth of dental unit water line biofilm isolates.

    Science.gov (United States)

    Liaqat, Iram; Bachmann, Robert Thomas; Sabri, Anjum Nasim; Edyvean, Robert G J

    2010-08-01

    Dental unit water line (DUWL) contamination by opportunistic pathogens has its significance in nosocomial infection of patients, health care workers, and life-threatening infections to immunocompromized persons. Recently, the quorum sensing (QS) system of DUWL isolates has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. In this study, the effect of two quorum-sensing inhibitory compounds (patulin; PAT, penicillic acid; PA) and EDTA on planktonic growth, AI-2 signalling and in vitro biofilm formation of Pseudomonas aeruginosa, Achromobacter xylosoxidans and Achromobacter sp. was monitored. Vibrio harveyi BB170 bioassay and crystal violet staining methods were used to detect the AI-2 monitoring and biofilm formation in DUWL isolates, respectively. The V. harveyi BB170 bioassay failed to induce bioluminescence in A. xylosoxidans and Achromobacter sp., while P. aeruginosa showed AI-2 like activity suggesting the need of some pretreatments prior to bioassay. All strains were found to form biofilms within 72 h of incubation. The QSIs/EDTA combination have isolate-specific effects on biofilm formation and in some cases it stimulated biofilm formation as often as it was inhibited. However, detailed information about the anti-biofilm effect of these compounds is still lacking.

  12. Optimization of lipase production by Staphylococcus sp. Lp12

    African Journals Online (AJOL)

    USER

    2010-02-08

    Feb 8, 2010 ... of the genera Pseudomonas, Bacillus, Staphylococcus,. Achromobacter have been cloned and characterized. Bacterial lipases are mostly inducible enzymes and require some form of oil, fatty acid, fatty acid alcohol or fatty acid ester and surfactants for induction (Immanuel et al., 2008). Lipase biosynthesis ...

  13. Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents

    DEFF Research Database (Denmark)

    Azevedo, Andreia S; Almeida, Carina; Pereira, Bruno

    2016-01-01

    Recently it was demonstrated that for urinary tract infections species with a lower or unproven pathogenic potential, such as Delftia tsuruhatensis and Achromobacter xylosoxidans, might interact with conventional pathogenic agents such as Escherichia coli. Here, single- and dual-species biofilms...... of these microorganisms were characterized in terms of microbial composition over time, the average fitness of E. coli, the spatial organization and the biofilm antimicrobial profile. The results revealed a positive impact of these species on the fitness of E. coli and a greater tolerance to the antibiotic agents....... In dual-species biofilms exposed to antibiotics, E. coli was able to dominate the microbial consortia in spite of being the most sensitive strain. This is the first study demonstrating the protective effect of less common species over E. coli under adverse conditions imposed by the use of antibiotic...

  14. Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents.

    Science.gov (United States)

    Azevedo, Andreia S; Almeida, Carina; Pereira, Bruno; Melo, Luís F; Azevedo, Nuno F

    2016-01-01

    Recently it was demonstrated that for urinary tract infections species with a lower or unproven pathogenic potential, such as Delftia tsuruhatensis and Achromobacter xylosoxidans, might interact with conventional pathogenic agents such as Escherichia coli. Here, single- and dual-species biofilms of these microorganisms were characterized in terms of microbial composition over time, the average fitness of E. coli, the spatial organization and the biofilm antimicrobial profile. The results revealed a positive impact of these species on the fitness of E. coli and a greater tolerance to the antibiotic agents. In dual-species biofilms exposed to antibiotics, E. coli was able to dominate the microbial consortia in spite of being the most sensitive strain. This is the first study demonstrating the protective effect of less common species over E. coli under adverse conditions imposed by the use of antibiotic agents.

  15. Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes

    DEFF Research Database (Denmark)

    Jakobsen, Tim Holm; Hansen, Martin Asser; Jensen, Peter Østrup

    2013-01-01

    Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans strain with phenotypic investigations of its important pathogenic features. We...... that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes....... In vitro studies of A. xylosoxidans NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing...

  16. Avaliação geoestatística do depósito estano-tungstenífero relacionado ao Maciço Granítico Correas-Ribeirão Branco-SP

    OpenAIRE

    José Maximino Tadeu Miras Ferron

    2000-01-01

    Esta dissertação apresenta os resultados de um estudo geoestatístico realizado sobre os dados de pesquisa em um depósito estano-tungstenífero relacionado ao Maciço Granítico Correas, localizado no Município de Ribeirão Branco - SP. Este depósito mineral foi intensivamente pesquisado pela Mineração Taboca S.A., em cooperação com o Instituto de Pesquisas Tecnológicas do Est. de São Paulo - IPT. Cerca de 5000 metros de sondagem rotativa a diamante foram realizados resultando na descoberta de sei...

  17. Porta-enxertos para o cultivar Máximo IAC 138-22 de uvas de vinho em Monte Alegre do Sul, SP

    OpenAIRE

    Terra, Maurilo Monteiro; Pires, Erasmo José Paioli; Coelho, Sônia Maria Bonilha Marcondes; Passos, Ilene Ribeiro da Silva; Santos, Rui Ribeiro dos; Pommer, Celso Valdevino; Silva, André Camargo Pereira da; Ribeiro, Ivan José Antunes

    1990-01-01

    Em Monte Alegre do Sul, SP, efetuou-se um experimento de longa duração para observar o comportamento do cultivar IAC 138-22 (Máximo) de uvas para vinho, sobre cinco porta-enxertos: RR 101-14, 'Golia', "Ripária do Traviú', IAC 572 e IAC 313. Em oito colheitas sucessivas anuais, de 1974 a 1981, analisaram-se as produções médias de uvas por planta. Os resultados indicaram que o IAC 572' foi o melhor porta-enxerto para o IAC 138-22 'Máximo'. O IAC 313 superou o IAC 572 em um dos anos estudados. T...

  18. ISP 22 OECD/NEA/CSNI International standard problem n. 22. Evaluation of post-test analyses

    International Nuclear Information System (INIS)

    1992-07-01

    The present report deals with the open re-evaluation of the originally double-blind CSNI International Standard Problem 22 based on the test SP-FW-02 performed in the SPES facility. The SPES apparatus is an experimental simulator of the Westinghouse PWR-PUN plant. The test SP-FW-02 (ISP22) simulates a complete loss of feedwater with delayed injection of auxiliary feedwater. The main parts of the report are: outline of the test facility and of the SP-FW-02 experiment; overview of pre-test activities; overview of input models used by post-test participants; evaluation of participant predictions; evaluation of qualitative and quantitative code accuracy of pre-test and post-test calculations

  19. Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22 and 26: Streptococcus parasuis sp. nov.

    Science.gov (United States)

    Nomoto, R; Maruyama, F; Ishida, S; Tohya, M; Sekizaki, T; Osawa, Ro

    2015-02-01

    In order to clarify the taxonomic position of serotypes 20, 22 and 26 of Streptococcus suis, biochemical and molecular genetic studies were performed on isolates (SUT-7, SUT-286(T), SUT-319, SUT-328 and SUT-380) reacted with specific antisera of serotypes 20, 22 or 26 from the saliva of healthy pigs as well as reference strains of serotypes 20, 22 and 26. Comparative recN gene sequencing showed high genetic relatedness among our isolates, but marked differences from the type strain S. suis NCTC 10234(T), i.e. 74.8-75.7 % sequence similarity. The genomic relatedness between the isolates and other strains of species of the genus Streptococcus, including S. suis, was calculated using the average nucleotide identity values of whole genome sequences, which indicated that serotypes 20, 22 and 26 should be removed taxonomically from S. suis and treated as a novel genomic species. Comparative sequence analysis revealed 99.0-100 % sequence similarities for the 16S rRNA genes between the reference strains of serotypes 20, 22 and 26, and our isolates. Isolate STU-286(T) had relatively high 16S rRNA gene sequence similarity with S. suis NCTC 10234(T) (98.8 %). SUT-286(T) could be distinguished from S. suis and other closely related species of the genus Streptococcus using biochemical tests. Due to its phylogenetic and phenotypic similarities to S. suis we propose naming the novel species Streptococcus parasuis sp. nov., with SUT-286(T) ( = JCM 30273(T) = DSM 29126(T)) as the type strain. © 2015 IUMS.

  20. Seletividade fisiológica de inseticidas aos inimigos naturais de Plutella xylostella (L. (Lepidoptera: Plutellidae em brássicas Insecticide physiological selectivity to natural enemies of Plutella xylostella (L. (Lepidoptera: Plutellidae in Brassicae

    Directory of Open Access Journals (Sweden)

    Leandro Bacci

    2009-01-01

    Full Text Available A conservação de inimigos naturais é um componente fundamental no manejo integrado de pragas. Neste trabalho, estudou-se a seletividade de sete inseticidas usados no manejo de Plutella xylostella (L. (Lepidoptera: Plutellidae ao parasitóide Oomyzus sokolowskii (Kurdjumov (Hymenoptera: Eulophidae e aos predadores Discodon sp. (Coleoptera: Cantharidae e Lasiochilus sp. (Hemiptera: Anthocoridae. Os organofosforados metamidofós e paratiom metílico e o carbamato carbaril foram altamente tóxicos aos três inimigos naturais estudados. Dimetoato apresentou seletividade em favor do Discodon sp., na dose e subdose. O inseticida acefato apresentou alto impacto ao Discodon sp., no momento da aplicação e seu efeito foi reduzido com a metade da concentração do princípio ativo. Deltametrina apresentou seletividade à O. sokolowskii nas duas dosagens. Já permetrina foi seletiva a Lasiochilus sp., somente na subdose. Discodon sp. foi mais tolerante à dose e subdose do dimetoato e à subdose do acefato do que O. sokolowskii e Lasiochilus sp. O predador Lasiochilus sp. foi mais tolerante à dose e subdose da permetrina do que O. sokolowskii e Discodon sp. O parasitóide O. sokolowskii foi mais tolerante à dose e subdose da deltametrina do que Lasiochilus sp. e Discodon sp. Os resultados desta pesquisa foram obtidos em condições de exposição extrema, portanto indicam que os inseticidas seletivos possivelmente não serão prejudiciais aos inimigos naturais, em condições de campo.The conservation of natural enemies is a fundamental component in the integrated pest management. In this work, one studied the selectivity of seven insecticides, used in the management of Plutella xylostella (L. (Lepidoptera: Plutellidae, to the parasitoid Oomyzus sokolowskii (Kurdjumov (Hymenoptera: Eulophidae, and to the predators Discodon sp. (Coleoptera: Cantharidae and Lasiochilus sp. (Hemiptera: Anthocoridae. The organophosphates methamidophos and methyl parathion

  1. Direct estimation of the oxygen requirements of Achromobacter xylosoxidans for aerobic degradation of monoaromatic hydrocarbons (BTEX) in a bioscrubber.

    Science.gov (United States)

    Nielsen, David R; McLellan, P James; Daugulis, Andrew J

    2006-08-01

    The O2 requirements for biomass production and supplying maintenance energy demands during the degradation of both benzene and ethylbenzene by Achromobacter xylosoxidans Y234 were measured using a newly proposed technique involving a bioscrubber. Using this approach, relevant microbial parameter estimates were directly and simultaneously obtained via linear regression of pseudo steady-state data. For benzene and ethylbenzene, the biomass yield on O2, Y(X/O2), was estimated on a cell dry weight (CDW) basis as 1.96 +/- 0.25 mg CDW mgO2(-1) and 0.98 +/- 0.17 mg CDW mgO2(-1), while the specific rate of O2 consumption for maintenance, m(O2), was estimated as 0.041 +/- 0.008 mgO(2) mg CDW(-1) h(-1) and 0.053 +/- 0.022 mgO(2) mg CDW(-1) h(-1), respectively.

  2. First report of a cross-kingdom pathogenic bacterium, Achromobacter xylosoxidans isolated from stipe-rot Coprinus comatus.

    Science.gov (United States)

    Ye, Luona; Guo, Mengpei; Ren, Pengfei; Wang, Gangzheng; Bian, Yinbing; Xiao, Yang; Zhou, Yan

    2018-03-01

    Coprinus comatus is an edible mushroom widely cultivated in China as a delicious food. Various diseases have occurred on C. comatus with the cultivated area increasing. In this study, the pathogenic bacterium JTG-B1, identified as Achromobacter xylosoxidans by 16S rDNA and nrdA gene sequencing, was isolated from edible mushroom Coprinus comatus with serious rot disease on its stipe. A. xylosoxidans has been confirmed as an important opportunistic human pathogenic bacterium and has been isolated from respiratory samples from cystic fibrosis. It is widely distributed in the environment. Here, we first report that fungi can also serve as a host for A. xylosoxidans. We confirmed that it can cross-kingdom infect between animals (mice) and fungi (C. comatus). The results of pathogenicity tests, physiological, biochemical and genotyping analysis of A. xylosoxidans from different hosts suggested that different strain of A. xylosoxidans may have pathogenicity differentiation. A. xylosoxidans not only is pathogenic to C. comatus but also may threaten human health. Copyright © 2017 Elsevier GmbH. All rights reserved.

  3. Cisalhamento na Linha de Cola de Eucalyptus sp. Colado com Diferentes Adesivos e Diferentes Gramaturas

    Directory of Open Access Journals (Sweden)

    Juliana Jerásio Bianche

    2017-08-01

    Full Text Available RESUMO O objetivo deste trabalho foi avaliar a resistência ao cisalhamento na linha de cola de juntas de Eucalyptus sp. coladas com diferentes adesivos e diferentes gramaturas. Para a colagem das juntas foi utilizada a madeira de Eucalyptus sp., dois adesivos de cura a quente (fenol-formaldeído e melamina-formaldeído, quatro adesivos de cura a frio [resorcinol-formaldeído, silicato de sódio, PVA (acetato de polivinila e poliuretano à base de mamona], empregando-se gramaturas de 150 g, 200 g e 250 g/m2 em face dupla. Foram determinados aresistência ao cisalhamento e à falha na madeira, e cisalhamento em condição úmida. Os resultados obtidos indicaram que os adesivos de mamona e resorcinol apresentaram maior resistência ao cisalhamento na linha de cola na condição seca. Os adesivos de mamona e fenol-formaldeído apresentaram maior percentual de falha na madeira na condição seca.

  4. Performance and bromatologic analysis of red tail lambari Astyanax sp F (Pisces: characidae submitted to the smoking process / Rendimento e análise bromatológica do lambari do rabo vermelho Astyanax sp F (Pisces: characidae submetido ao processo de defumação

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Weirich

    2009-12-01

    Full Text Available The aim of this study was to evaluate the time of hot smoked and the influence of carcass quality of the red tail lambari Astyanax sp F. Sixty lambaris, eviscerated with head, skin, bones, spinal column, fins, however, without scale. With a medium weight and length of 22.22±4.32g and 11.32±00.63cm were utilized. The animals were submitted to humid salted (25% of sodium chloride for 45 minutes and condiments, been later hot smoked for a period of 90, 180, 270, 360 min. After the smoking process, the fish were weighted and later the humidity tenor, gross protein, lipids and mineral matter of the carcass of the animals were calculated. The results showed differences (P O objetivo do presente trabalho foi avaliar o tempo de defumação a quente e a influência sobre a qualidade de carcaça de lambaris Astyanax sp. F. Foram utilizados 60 lambaris, com peso e comprimento médio de 22,22±4,32g e 11,32±0,63cm, eviscerados e com cabeça, pele, espinhas, coluna vertebral, nadadeiras, porém, sem escamas. Os animais foram submetidos à salga úmida (25% de cloreto de sódio por 45 minutos e condimentos, sendo posteriormente defumados a quente, por um período de 90, 180, 270 e 360min. Após o processo de defumação, os peixes foram pesados e posteriormente calculados os teores de umidade, proteína bruta, lipídeos e matéria mineral da carcaça dos animais. Os resultados mostraram diferenças (P 0,05 entre os diferentes tempos de defumação. Portanto, conclui-se que para o Astyanax sp F o tempo de defumação de 225min7seg é o período de defumação indicado para o melhor produto final.

  5. Ovos de Toxocara sp. e larvas de Ancylostoma sp. em praça pública de Lavras, MG Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil

    Directory of Open Access Journals (Sweden)

    Antônio Marcos Guimarães

    2005-04-01

    Full Text Available Larva migrans visceral e cutânea são zoonoses parasitárias causadas pela infecção da larva de Toxocara sp. e Ancylostoma sp., respectivamente. O objetivo do estudo foi verificar a contaminação por ovos de Toxocara sp. e ovos e larvas de Ancylostoma sp. em amostras de solos coletadas de praças públicas e de áreas de recreação infantil de Lavras, Estado de Minas Gerais, por meio da técnica de centrífugo-flutuação e do método de Baermann. A ocorrência de ovos de Toxocara sp. e, ovos e larvas de Ancylostoma sp. foi observada em 69,6% (16/23 das amostras de solo coletadas de praças públicas. A contaminação somente por ovos de Ancylostoma sp. em amostras de solo coletadas em escolas/creches foi de 22,2% (4/18. A percentagem de amostras de areia coletadas de escolas/creches contaminadas somente com larvas de Ancylostoma sp. foi de 11,1% (2/18. Praças públicas são as áreas com maior risco potencial de infecção por Toxocara sp. e Ancylostoma sp. Exame coproparasitológico realizado em 174 amostras de fezes de cães observou 58% e 23%, respectivamente, com ovos de Ancylostoma sp. e Toxocara sp.Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23 and 69.6 (16/23 respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18. Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool

  6. Dimorfismo de ascósporos em Glomerella cingulata f.sp. phaseoli

    OpenAIRE

    Castro, Renata A.; Mendes-Costa, Maria C.; Souza, Elaine A.

    2006-01-01

    O objetivo deste trabalho foi caracterizar linhagens de Glomerella cingulata f. sp. phaseoli quanto ao crescimento micelial, formação de peritécio e dimorfismo de ascósporos. Foram avaliadas quatro linhagens nos meios de cultura de folhas de feijoeiro e meio M3 e em condições com e sem fotoperíodo. O comprimento dos ascósporos da linhagem normal e da mutante foi determinado. Houve diferença significativa quanto ao crescimento micelial e tamanho dos ascósporos. Constatou-se, pela primeira vez,...

  7. Magnetic bionanoparticles of Penicillium sp. yz11-22N2 doped with Fe3O4 and encapsulated within PVA-SA gel beads for atrazine removal.

    Science.gov (United States)

    Yu, Jiaping; He, Huijun; Yang, William L; Yang, Chunping; Zeng, Guangming; Wu, Xin

    2018-07-01

    A novel magnetic bionanomaterial, Penicillium sp. yz11-22N2 doped with nano Fe 3 O 4 entrapped in polyvinyl alcohol-sodium alginate gel beads (PFEPS), was successfully synthesized. The factors including nutrient substance, temperature, pH, initial concentrations of atrazine and rotational speeds were presented and discussed in detail. Results showed that the highest removal efficiency of atrazine by PFEPS was 91.2% at 8.00 mg/L atrazine. The maximum removal capacity for atrazine was 7.94 mg/g. Meanwhile, it has been found that most of atrazine were removed by metabolism and degradation of Penicillium sp. yz11-22N2, which could use atrazine as the sole source of either carbon or nitrogen. Degradation kinetics of atrazine conformed to first-order kinetics model. The intermediates indicated that the possible pathway for atrazine degradation by PFEPS mainly included hydrolysis dechlorination, dealkylation, side-chain oxidation and ring-opening. Copyright © 2018 Elsevier Ltd. All rights reserved.

  8. AoS28D, a proline-Xaa carboxypeptidase secreted by Aspergillus oryzae.

    Science.gov (United States)

    Salamin, Karine; Eugster, Philippe J; Jousson, Olivier; Waridel, Patrice; Grouzmann, Eric; Monod, Michel

    2017-05-01

    Prolyl peptidases of the MEROPS S28 family are of particular interest because they are key enzymes in the digestion of proline-rich peptides. A BLAST analysis of the Aspergillus oryzae genome revealed sequences coding for four proteases of the S28 family. Three of these proteases, AoS28A, AoS28B, and AoS28C, were previously characterized as acidic prolyl endopeptidases. The fourth protease, AoS28D, showed high sequence divergence with other S28 proteases and belongs to a phylogenetically distinct cluster together with orthologous proteases from other Aspergillus species. The objective of the present paper was to characterize AoS28D protease in terms of substrate specificity and activity. AoS28D produced by gene overexpression in A. oryzae and in Pichia pastoris was a 70-kDa glycoprotein with a 10-kDa sugar moiety. In contrast with other S28 proteases, AoS28D did not hydrolyze internal Pro-Xaa bonds of several tested peptides. Similarly, to human lysosomal Pro-Xaa carboxypeptidase, AoS28D demonstrated selectivity for cleaving C-terminal Pro-Xaa bonds which are resistant to carboxypeptidases of the S10 family concomitantly secreted by A. oryzae. Therefore, AoS28D could act in synergy with these enzymes during sequential degradation of a peptide from its C-terminus.

  9. Fungicidal activities and mechanisms of action of Pseudomonas syringae pv. syringae lipodepsipeptide syringopeptins 22A and 25A

    Directory of Open Access Journals (Sweden)

    Mekki F. Bensaci

    2011-10-01

    Full Text Available The plant-associated bacterium Pseudomonas syringae pv. syringae simultaneously produces two classes of metabolites: the small cyclic lipodepsinonapeptides such as the syringomycins and the larger cyclic lipodepsipeptide syringopeptins SP22 or SP25. The syringomycins inhibit a broad spectrum of fungi (but particularly yeasts by lipid-dependent membrane interaction. The syringopeptins are phytotoxic and inhibitory to Gram positive bacteria. In this study, the fungicidal activities of two major syringopeptins, SP22A and SP25A, and their mechanisms of action were investigated and compared to those of syringomycin E. SP22A and SP25A were observed to inhibit the fungal yeasts Saccharomyces cerevisiae and Candida albicans although less effectively than syringomycin E. S. cerevisiae mutants defective in ergosterol and sphingolipid biosyntheses were less susceptible to SP22A and SP25A but the relative inhibitory capabilities of SRE vs. SP22A and SP25A were maintained. Similar differences were observed for capabilities to cause cellular K+ and Ca2+ fluxes in S. cerevisiae. Interestingly, in phospholipid bilayers the syringopeptins are found to induce larger macroscopic ionic conductances than syringomycin E but form single channels with similar properties. These findings suggest that the syringopeptins target the yeast plasma membrane, and, like syringomycin E, employ a lipid-dependent channel forming mechanism of action. The differing degrees of growth inhibition by these lipodepsipeptides may be explained by differences in their hydrophobicity. The more hydrophobic SP22A and SP25A might interact more strongly with the yeast cell wall that would create a selective barrier for their incorporation into the plasma membrane.

  10. Diabetes Mellitus: desafios relacionados ao autocuidado abordados em Grupo de Apoio Psicológico

    Directory of Open Access Journals (Sweden)

    Nunila Ferreira de Oliveira

    2011-04-01

    Full Text Available O objetivo foi identificar os sentimentos dos usuários associados ao diagnóstico do Diabetes Mellitus (DM, as dificuldades inerentes ao controle glicêmico e estratégias de enfrentamento adotadas diante das demandas impostas pela doença. Estudo qualitativo realizado com grupo de pessoas acompanhadas por equipe multiprofissional de um centro de pesquisa e extensão universitária de Ribeirão Preto, SP, Brasil. Quatro sessões grupais que constituíram o corpus da pesquisa, foram audiogravadas e transcritas. A análise temática originou as categorias: sentimentos despertados a partir do diagnóstico do DM; dificuldades no controle da doença e estratégias de enfrentamento. Os resultados evidenciaram diferentes modos de lidar com os desafios suscitados pela doença no cotidiano, que influenciam diretamente na adesão ao tratamento e constituem elementos relevantes para o planejamento de intervenções mais efetivas.

  11. SOJA IAC/IAS-5: CULTIVAR RESISTENTE AO CANCRO DA HASTE

    Directory of Open Access Journals (Sweden)

    MASCARENHAS HIPÓLITO ASSUNÇÃO ANTONIO

    1998-01-01

    Full Text Available Há aproximadamente oito anos, o cancro da haste (Phomopsis phaseoli f. sp. meridionalis/Diaporthe phaseolorum f. sp. meridionalis da soja tem sido o principal fator limitante da produtividade em áreas de manifestação da doença. O seu controle mediante cultivares resistentes é o método mais econômico. Num ensaio em casa de vegetação, visando avaliar o efeito de doses de potássio no controle do cancro da haste, no cultivar IAS-5, moderadamente resistente, verificou-se haver plantas altamente resistentes, comprovadas em três gerações, todas inoculadas com o fungo causador da doença. A importância da obtenção do IAS-5 e de sua multiplicação deve-se a ser o mais cultivado no Estado de São Paulo e apresentar resistência ao cancro da haste.

  12. Phytotoxic eremophilane sesquiterpenes from the coprophilous fungus Penicillium sp. G1-a14.

    Science.gov (United States)

    Del Valle, Paulina; Figueroa, Mario; Mata, Rachel

    2015-02-27

    Bioassay-directed fractionation of an extract from the grain-based culture of the coprophilous fungus Penicillium sp. G1-a14 led to the isolation of a new eremophilane-type sesquiterpene, 3R,6R-dihydroxy-9,7(11)-dien-8-oxoeremophilane (1), along with three known analogues, namely, isopetasol (2), sporogen AO-1 (3), and dihydrosporogen AO-1 (4). The structure of 1 was elucidated using 1D and 2D NMR and single-crystal X-ray diffraction. Assignment of absolute configuration at the stereogenic centers of 1 was achieved using ECD spectroscopy combined with time-dependent density functional theory calculations. Sporogen AO-1 (3) and dihydrosporogen AO-1 (4) caused significant inhibition of radicle growth against Amaranthus hypochondriacus (IC50 = 0.17 mM for both compounds) and Echinochloa crus-galli (IC50 = 0.17 and 0.30 mM, respectively).

  13. Formulation of a minimal nutritional medium for enhanced lipid productivity in Chlorella sp. and Botryococcus sp. using response surface methodology.

    Science.gov (United States)

    Vishwakarma, Rashi; Dhar, Dolly Wattal; Pabbi, Sunil

    2018-03-01

    Chlorella sp. MCC 7 and Botryococcus sp. MCC 31 were investigated to enable large-scale biodiesel production from minimal constituents in the growth medium. Response surface methodology (RSM) was used to maximise the biomass productivity and lipid yield using only nitrogen (N), phosphorus (P) and potassium (K) as urea, single super phosphate and muriate of potash. The optimum values were 0.42 g/L nitrogen; 0.14 g/L phosphorus and 0.22 g/L potassium for Chlorella sp.; and 0.46 g/L; 0.14 g/L and 0.25 g/L for Botryococcus sp. Lipid yield of 42% for Chlorella sp. and 52% in Botryococcus sp. was observed. An enhancement in lipid yield by approximately 55% for Chlorella sp. and 73% for Botryococcus sp. was registered as compared to original nutrient medium. Fourier transform infrared (FTIR) analysis of extracted lipids revealed characteristic bands for triglycerides. This study provided utilisation of a practicable nutrient recipe in the form of N, P, K input for enhanced lipid yield from the selected microalgal strains.

  14. NCBI nr-aa BLAST: CBRC-TNIG-22-0088 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0088 ref|YP_001278502.1| hypothetical protein RoseRS_4209 [Roseiflexus... sp. RS-1] gb|ABQ92552.1| hypothetical protein RoseRS_4209 [Roseiflexus sp. RS-1] YP_001278502.1 0.072 28% ...

  15. NCBI nr-aa BLAST: CBRC-TNIG-22-0157 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0157 ref|ZP_01915505.1| hypothetical protein LMED105_09745 [Limnobacter... sp. MED105] gb|EDM83186.1| hypothetical protein LMED105_09745 [Limnobacter sp. MED105] ZP_01915505.1 3e-15 31% ...

  16. AÇÃO ANTIFÚNGICA in vitro DE ISOLADOS DE Bacillu s sp. SOBRE Fusarium oxysporum f. sp. lycopersici

    Directory of Open Access Journals (Sweden)

    ODENILSON DE DEUS RIBEIRO LIMA

    2014-01-01

    Full Text Available This study aimed to evaluate antagonism and metabolites produced by different species of Ba- cillus in the inhibition of mycelial growth in vitro against F. oxysporum f. sp . lycopersici . For evaluating the antagonism of Bacillus spp. F. oxysporum f. sp . lycopersici was performed pairing of fungus and bacteria by the method of the circle. In the method for detection for the quality for thermostable metabolites liquids. Media BD were used for growth of the isolated Bacillus sp. And incubated for 15 days. After this period, was added 3 g of agar in each flask, and autoclaved broth and poured into Petri dishes. In the center of the plates were placed discs culture of the pathogen. The experimental design was completely randomized with 11 treatments and six repetitions in both experiments. Statistical difference was found between the isolate and the control. Special mention to strains B12 ( Bacillus sp., B41 ( B. cereus , B22' ( B.pentothenticus , B45 ( B. cereus , B47 ( B. cereus that exhibited the lowest average diameter of the colony. To study the inhibition of mycelial growth of F. oxysporum f. sp. lycopersici by thermostatable metabolites five differ statistically from the control they are: B35 ( B. pumilus , B47 ( B. cereus , B22' ( B. pentothenticus , B12 ( Bacillus sp. and B41 ( B. cereus the latter two treatments showed the best results of the pathogen colony diameters and 3.81 to 2.89 cm, respective- ly. B12 and B41 Isolates showed that their antibiotic products were able to inhibit 67.88 % and 57,66 % of F. oxysporum f. sp. lycopersici . These results highlight the possibility of using isolates of the genus Bacillus in the fight against fusarium wilt in tomato.

  17. Porta-enxertos para o cultivar Máximo IAC 138-22 de uvas de vinho em Monte Alegre do Sul, SP Rootstocks for wine grape cultivar IAC 138-22

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    Maurilo Monteiro Terra

    1990-01-01

    Full Text Available Em Monte Alegre do Sul, SP, efetuou-se um experimento de longa duração para observar o comportamento do cultivar IAC 138-22 (Máximo de uvas para vinho, sobre cinco porta-enxertos: RR 101-14, 'Golia', "Ripária do Traviú', IAC 572 e IAC 313. Em oito colheitas sucessivas anuais, de 1974 a 1981, analisaram-se as produções médias de uvas por planta. Os resultados indicaram que o IAC 572' foi o melhor porta-enxerto para o IAC 138-22 'Máximo'. O IAC 313 superou o IAC 572 em um dos anos estudados. Todos os outros porta-enxertos apresentaram desempenho inferior.A long term experiment was conducted at Monte Alegre do Sul, State of São Paulo, Brazil, to study the behaviour of wine grape cultivar IAC 138-22 Máximo on five rootstocks: RR 101 14, 'Golia', "Ripária do Traviú', IAC 313 and IAC 572 The yields of eight consecutive annual harvests (from 1974 to 1981 were analysed. The results indicated that IAC 572 was the best rootstock. IAC 313 can also be used but with fair results. "Ripária do Traviú', RR 101-14 and 'Golia' are not recommended as rootstocks for IAC 138-22. Considering the row spacing (2 x 1m which allows 5,000 plants/ha, the difference between average yields of the best and the worst rootstock was 8,475kg/ha of grapes, a considerable figure for the grape grower.

  18. Improved production of an enzyme that hydrolyses raw yam starch by Penicillium sp. S-22 using fed-batch fermentation.

    Science.gov (United States)

    Sun, Hai-Yan; Ge, Xiang-Yang; Zhang, Wei-Guo

    2006-11-01

    A newly isolated strain, Penicillium sp. S-22, was used to produce an enzyme that hydrolyses raw yam starch [raw yam starch digesting enzyme (RYSDE)]. The enzyme activity and overall enzyme productivity were respectively 16 U/ml and 0.19 U/ml h in the batch culture. The enzyme activity increased to 85 U/ml by feeding of partially hydrolyzed raw yam starch. When a mixture containing partially hydrolyzed raw yam starch and peptone was fed by a pH-stat strategy, the enzyme activity reached 366 U/ml, 23-fold of that obtained in the batch culture, and the overall productivity reached 3.4 U/ml h, which was 18-fold of that in the batch culture.

  19. CAPACIDAD ANTAGONISTA DE HONGOS CELULOLÍTICOS FRENTE A Fusarium sp. Y Macrophomina sp.

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    Néstor Leopoldo Tarazona Meza

    2014-12-01

    Full Text Available El objetivo de esta investigación fue evaluar la capacidad antagónica de ocho hongos celulolíticos nativos frente a los hongos fitopatógenos Fusarium sp. y Macrophomina sp. En primera instancia se determinó la cepa promisoria del hongo celulolítico, para lo cual, en caja Petri se colocó un disco de agar de 4 mm de diámetro con micelio de una cepa antagonista y en el extremo opuesto, a una distancia de cinco centímetros aproximadamente, otro disco de 4 mm con micelio de un patógeno. La capacidad antagónica se determinó por el grado de micoparasitismo y la competencia por nutrientes y espacios, registrando el porcentaje de inhibición del crecimiento radial. Con la cepa del hongo celulolítico que tuvo el mejor comportamiento antagonista se realizó un cocultivo independiente con cada patógeno, aquí se evalúo las UFC, crecidas en medio PDA. También se inoculó junto a las cepas fitopatógenas en plantas de pimiento para conocer el nivel de antagonismo en macetas. En la evaluación cualitativa, la cepa A.O-5 mostró una invasión y esporulación total en la superficie en ambos patógenos; mientras que en el cocultivo inhibe el crecimiento de los patógenos a las 96 h. Igualmente demostró su acción antagonista frente a Fusarium y Macrophomina en las plántulas de pimiento. Por lo encontrado, se estima que la cepa A.O-5 es un potencial agente antagonista de los fitopatógenos evaluados.

  20. NCBI nr-aa BLAST: CBRC-TNIG-22-0185 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0185 ref|ZP_01047391.1| putative manganese transport protein MntH [Nit...robacter sp. Nb-311A] gb|EAQ34662.1| putative manganese transport protein MntH [Nitrobacter sp. Nb-311A] ZP_01047391.1 0.11 28% ...

  1. Radiosensitivity of some bacteria isolated from broiler chicken carcasses

    International Nuclear Information System (INIS)

    Fiszer, W.; Mroz, J.; Zabielski, J.

    1981-01-01

    Two groups of bacteria of Pseudomonas sp. and Bacillus sp. were isolated from poultry carcasses. The samples of a ground meat were poisoned by suspensions of these bacteria and on the next day they were irradiated. Quantitative estimation of surviving cells after irradiation was done according to TPC method. The surviving curve and dose D 10 (23 Gy) for Pseudomonas sp. group 1 is typical for this kind of bacterium. D 10 value = 2,3 kGy for Bacillus sp. is consistent with data of literature. Exceptionally high D 10 value of Pseudomonas sp. group 2 (170 Gy) seems to indicate the fact that the isolated bacteria could be the mixture often defined as Pseudomonas-Achromobacter group. Nevertheless, some scientific data show that D 10 value for Pseudomonas can reach even 120 Gy. (author)

  2. Draft genome sequences of six neonatal meningitis-causing escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65)

    Science.gov (United States)

    Neonatal meningitis Escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65) were recovered from infants in the Netherlands from 1989 to 1997. Here, we report the draft genome sequences for these six E. coli isolates, which are currently being used to validate food safety processing te...

  3. Avaliação da contaminação por enteroparasitas em alfaces (lactuca sativa comercializadas em restaurantes com alimentos expostos ao consumo no município de Sorocaba/SP

    Directory of Open Access Journals (Sweden)

    Caio Vieira Magalhães Ornelas

    2015-10-01

    Full Text Available Diante de um cotidiano pauta do por uma intensa influência dos meios decomunicação a respeito da alimentação saudável e de baixo teor calórico,bem como de um progressivo número de campanhas sociais combatendoa obesidade e seu alto valor epidemiológico sobre a sociedadecontemporânea, as hortaliças têm recebido ampla ênfase no cenárionutricional dos dias de hoje. Entretanto, tais aspectos são tambémacompanhados de extrema cautela, uma vez que as hortaliças sãoimportantes veículos de infecções ao organismo humano através de umavasta gama de possíveis parasitas, devido à manipulação pré-consumo einclusive o consumo cru. O trabalho que se segue teve como objetivopesquisar a presença de enteroparasitas especificamente em alfacescomercializadas em restaurantes com alimentos expostos ao consumo nomunicípio de Sorocaba-SP. As amostras de alface crespa (Lactuca sativacoletadas foram submetidas a uma série de etapas para análise:inicialmente houve lavagem em sacos plásticos durante 30 minutos,seguida de sedimentação do líquido por 24 horas e, por fim, análiseparasitológica da amostra por microscopia óptica. Das 40 amostrasrealizadas, apenas 5% continham enteroparasitas. Outras duas amostrascontinham achados como Paramecium spp. e ácaro. Os resultadosreforçam a necessidade de um a boa higienização das hortaliças, mas,devido ao grande número de restaurantes em Sorocaba comparado com ode amostras coletadas, é inadequado considerar generalizar a premissade que os restaurantes self-service possuam alfaces crespa (Lactucasativa higienizadas e próprias para o consumo em Sorocaba.

  4. Levantamento pedológico detalhado da estação experimental de Itararé, SP Detailed soil survey of the agricultural experiment station at Itararé, SP

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    J. Bertoldo de Oliveira

    1976-07-01

    Full Text Available O presente trabalho descreve o levantamento de solos ao nível de série, efetuado na Estação Experimental de Itararé, SP, que ocupa uma área de 495 ha. O relevo regional é representado por superfícies suavizadas esculpidas em material do Devoniano. O tipo climático é Cfa, segundo Koppen, e a vegetação primitiva, campos altimontanos. Foram observados 118 pontos em 50 dos quais foi coletado material para análise granulométrica e/ou química. Foram coletados, descritos e analisados 10 perfis e estabelecidas sete unidades de mapeamento pertencentes aos grandes grupos Inceptisol e Oxisol, e a um complexo indiscriminado.In this paper a description of a detailed soil survey in the Agricultural Experiment Station at Itararé, SP is given. The soil survey of the 495 ha of the Experiment Station was done at the series level. Seven soil units were identified and the description and analysis of four Inceptisols, and two Oxisols are presented. A soil map at 1:10,000 scale is also presented.

  5. Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov.

    Science.gov (United States)

    Pindi, Pavan Kumar; Kishore, K Hara; Reddy, G S N; Shivaji, S

    2009-06-01

    Strains KFC-22(T) and SPC-20(T) are yellow-pigmented, Gram-positive, aerobic, non-motile, rod-shaped bacteria that were isolated from a soil sample near the Kafni glacier in the Himalayan mountain ranges in India, and from a spade core sediment sample from the Antarctic Ocean at Larsemann Hill, respectively. In both cases, the cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid, anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) were the predominant fatty acids and MK-11 was the major isoprenoid quinone in the cell membrane. On the basis of the above-mentioned characteristics, both strains can be assigned to the genus Leifsonia. The strains share 16S rRNA gene sequence similarity of 97.7 % and DNA relatedness of only 10 %, indicating that they represent different species. A blast analysis indicated that Leifsonia pindariensis PON10(T) was the closest phylogenetic neighbour of strains SPC-20(T) and KFC-22(T), showing 16S rRNA gene sequence similarities of 97.3 and 97.7 %, respectively. However, at the whole-genome level, strains KFC-22(T) and SPC-20(T) shared 42 and 11 % DNA-DNA relatedness, respectively, with L. pindariensis PON10(T). In addition, both strains exhibited several phenotypic differences with respect to L. pindariensis PON10(T). Thus, on the basis of the differences that the two strains exhibited with respect to L. pindariensis, both were identified as representing novel species of the genus Leifsonia, for which the names Leifsonia kafniensis sp. nov. (type strain KFC-22(T) =NCCB 100216(T) =LMG 24362(T)) and Leifsonia antarctica sp. nov. (type strain SPC-20(T) =NCCB 100227(T) =LMG 24541(T)) are proposed.

  6. Avaliação do nível de conhecimento quanto ao uso de lentes de contato entre os estudantes de medicina da Faculdade de Medicina do ABC

    Directory of Open Access Journals (Sweden)

    Giovana Arlene Fioravanti Lui

    2010-12-01

    Full Text Available OBJETIVO: Analisar o nível de conhecimento dos usuários de lentes de contato entre os estudantes de medicina da Faculdade de Medicina do ABC (SP quanto aos cuidados, formas de uso, às complicações e à aquisição das lentes. MÉTODOS: Foi aplicado um questionário a todos os alunos usuários de lentes de contato do 1º ao 6º ano de medicina da Faculdade de Medicina do ABC (SP, analisando-se por fim os resultados encontrados. Todos os questionários aplicados foram respondidos. RESULTADOS: 61 estudantes responderam ao questionário. 52,5% afirmaram já terem tido algum tipo de complicação ocular decorrente do uso de lentes de contato. Apesar de a maioria (70,5% estar satisfeito com o uso das lentes de contato, 67% dos que responderam ao questionário estão esperando o grau estabilizar para realizar cirurgia para correção de grau. CONCLUSÃO: Observou-se uma adaptação inadequada, aquisição de lentes de contato em ópticas ou farmácias e complicações oculares graves ocorreram em usuários de lentes de contato considerados diferenciados. Podemos concluir que o médico oftalmologista deverá dar mais atenção ao tema, para que haja menos complicações ao realizar adaptações de lentes de contato.

  7. Potential of wheat bran to promote indigenous microbial enhanced oil recovery.

    Science.gov (United States)

    Zhan, Yali; Wang, Qinghong; Chen, Chunmao; Kim, Jung Bong; Zhang, Hongdan; Yoza, Brandon A; Li, Qing X

    2017-06-01

    Microbial enhanced oil recovery (MEOR) is an emerging oil extraction technology that utilizes microorganisms to facilitate recovery of crude oil in depleted petroleum reservoirs. In the present study, effects of wheat bran utilization were investigated on stimulation of indigenous MEOR. Biostimulation conditions were optimized with the response surface methodology. The co-application of wheat bran with KNO 3 and NH 4 H 2 PO 4 significantly promoted indigenous MEOR (IMEOR) and exhibited sequential aerobic (O-), facultative (A n -) and anaerobic (A 0 -) metabolic stages. The surface tension of fermented broth decreased by approximately 35%, and the crude oil was highly emulsified. Microbial community structure varied largely among and in different IMEOR metabolic stages. Pseudomonas sp., Citrobacter sp., and uncultured Burkholderia sp. dominated the O-, A n - and early A 0 -stages. Bacillus sp., Achromobacter sp., Rhizobiales sp., Alcaligenes sp. and Clostridium sp. dominated the later A 0 -stage. This study illustrated occurrences of microbial community succession driven by wheat bran stimulation and its industrial potential.

  8. Botanical pesticides effect from shells of bean’s cashew nut on biological agents of trichoderma sp. and gliocladium sp.

    Science.gov (United States)

    Bande, L. O. S.; Mariadi; Gusnawaty, HS; Nuriadi; Trisulpa, L.; Rahmania

    2018-02-01

    A shell of cashew nut (Anacardium occidentanle) has contained Cashew Nut Shell Liquid (CNSL) that is used as botanical pesticides. CNSL oil consists of active substance such as anacardat acid, cardol and cardanol. Utilization of the pesticides from shells of cashew nut to control pests and diseases of plants would be affected on biological agents. The objective of this research was to investigate pesticides inhibition on the increase of mycelium Trichoderma sp. and Gliocladium sp. by in vitro method. The tested concentration sample consisted of 0.0% (control), 2.5%, 7.5% and 10.0% in PDA media. The results of this research showed that 2.5% botanical pesticides concentration could minimize mycelium of Trichoderma sp. and Gliocladium sp. 22.73% and 21.04% respectively and also the increase shells of cashew extract could be affected the increase of mycelium inhibition. The extract with 2.5% concentration was the recommended concentration to control of fruit rot diseases and if concentration was 10.0% then its inhibition become 54.98% and 49.35%, respectively. The results proved that uncontrolled utilization of the pesticides could be affected on decrease of Trichoderma sp. and Gliocladium sp. growth.

  9. Prevenção da Aids com adolescentes encarcerados em São Paulo, SP

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    Camila Alves Peres

    2002-08-01

    Full Text Available OBJETIVOS: Descrever o perfil de adolescentes quanto ao apoio social e familiar, ao uso de drogas e os conhecimentos, as práticas e atitudes relacionadas à Aids e sua prevenção. MÉTODOS: Foram estudados 275 jovens internos, do sexo masculino, de um centro de internação da Fundação Estadual do Bem Estar do Menor (Febem, em São Paulo, SP. A pesquisa foi feita em duas fases: a primeira por meio de entrevistas semi-estruturadas com 20 internos; a segunda, com questionários para auto-respostas aplicados aos 275 internos, com perguntas fechadas referentes a características sociodemográficas, criminalidade, práticas sexuais, uso de drogas, conhecimento, atitudes e práticas relativas à Aids. RESULTADOS: Do total estudado, 90% dos jovens internos residiam com suas famílias antes da internação; todos haviam estudado em escolas públicas, ainda que 61% já houvessem abandonado os estudos; 12% já haviam usado drogas; e 5,5% eram usuários de drogas intravenosas. A maioria (98% era sexualmente ativa; 35% haviam tido mais de 15 parceiras(os sexuais ao longo da vida; 8% haviam tido experiências homossexuais (dentro ou fora da Febem; 12% já haviam trocado sexo por benefícios materiais; e 22% já eram pais. Muitos dos adolescentes afirmaram que adquirir o HIV "é parte da vida" e que suas vidas apresentam riscos piores, como sobreviver na criminalidade. Acreditam que o preservativo é frágil (83% e atrapalha a relação sexual (58%; 72% já haviam utilizado preservativo, mas apenas 9% o utilizavam sempre. CONCLUSÕES: Os adolescentes apresentaram um elevado risco de aquisição do HIV. Assim, torna-se necessário integrar a prevenção da Aids em sua problemática de vida e em temas como racismo, esperança pelo futuro, criminalidade, uso de drogas, direitos fundamentais, incluídos nestes os referentes ao sexo e à reprodução, mostrando existir alternativas a adquirir o HIV ou morrer na criminalidade.

  10. Seletividade de inseticidas a Doru luteipes (Scudder, 1876 (Dermaptera: Forficulidae e Cotesia sp. (Hymenoptera: Braconidae inimigos naturais de Ascia monuste orseis (Godart, 1818 (Lepdoptera: Pieridae Selectivity of insecticides to Doru luteipes (Scudder, 1876 (Dermaptera: Forficulidae and Cotesia sp. (Hymenoptera: Braconidae natural enemies of Ascia monuste orseis (Godart, 1818 (Lepdoptera: Pieridae

    Directory of Open Access Journals (Sweden)

    Marcelo Coutitnho Picanço

    2003-04-01

    Full Text Available Este trabalho objetivou estudar a seletividade dos inseticidas carbaril, deltametrina, paratiom metílico, permetrina e triclorfom em favor do predador Doru luteipes (Scudder (Dermaptera: Forficulidae e do parasitóide Cotesia sp. (Hymenoptera: Braconidae, em relação ao controle do curuquerê Ascia monuste orseis (Godart (Lepidoptera: Pieridae. Foram determinadas as CL90 para A. monuste orseis e estas foram utilizadas como doses discriminatórias para se avaliar o grau de seletividade dos inseticidas. Deltametrina e permetrina foram altamente seletivos em favor de D. luteipes. Deltametrina foi medianamente seletiva em favor de Cotesia sp. e a permetrina não apresentou seletividade em favor deste parasitóide. Triclorfom foi altamente seletivo em favor de ninfas de quarto estádio e adultos de D. luteipes e mediamente seletivo em favor de Cotesia sp. e de ninfas de primeiro estádio do predador. Carbaril foi medianamente seletivo em favor de Cotesia sp. mas não apresentou seletividade em favor de D. luteipes. Paratiom metílico não foi seletivo em favor de Cotesia sp. e D. luteipes. Adultos de D. luteipes foram menos sensíveis a deltametrina, permetrina e triclorfom do que adultos de Cotesia sp., sendo que o inverso ocorreu com o carbaril. Esses inimigos naturais apresentaram alta sensibilidade ao paratiom metílico. Ninfas de quarto estádio e adultos de D. luteipes foram mais sensíveis ao paratiom metílico e triclorfom que ninfas de primeiro estádio. Ninfas e adultos de D. luteipes apresentaram semelhante sensibilidade ao carbaril, deltametrina e permetrina.This work aimed to study the selectivity of the insecticides carbaryl, deltamethrin, methyl parathion, permethrin and trichlorfon in favor of the predator Doru luteipes (Scudder (Dermaptera: Forficulidae and of the parasitoid Cotesia sp. (Hymenoptera; Braconidae in relation to the control of the kale leafworm Ascia monuste orseis (Godart (Lepidoptera: Pieridae. LC90 were determined

  11. Antimicrobial susceptibility of Campylobacter sp strains isolated from calves with and without diarrhea in Minas Gerais state, Brazil Susceptibilidade a antimicrobianos de amostras de Campylobacter sp isoladas de bezerros com e sem diarréia, no estado de Minas Gerais, Brasil

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    Karina Leite Miranda

    2007-06-01

    Full Text Available The antimicrobial susceptibility of 25 Campylobacter sp strains isolated from calves with and without diarrhea - 7 C. coli, 16 C. fetus and 2 C. jejuni was studied by the disk diffusion method. Eleven antimicrobial agents were tested amikacin, ampicillin, kanamycin, chloramphenicol, erythromycin, gentamicin, neomycin, nitrofurantoin, penicillin G, tetracycline and sulfamethoxazole-trimethoprim. All Campylobacter sp strains were susceptible to amikacin, ampicillin, chloramphenicol, erythromycin, gentamicin, neomycin and nitrofurantoin. Three strains were moderately susceptible to kanamycin (2 C. coli and 1 C. fetus. All the strains were resistant to penicillin G. Two C. fetus strains were moderately susceptible to sulfamethoxazole-trimethoprim and 1 C. coli, 9 C. fetus and 2 C. jejuni strains were resistant. Two C. fetus strains were moderately susceptible to tetracycline and 3 C. coli, 2 C. fetus and 1 C. jejuni strains were resistant. Eleven strains showed multidrug resistance (2 C. coli, 8 C. fetus and 1 C. jejuni. There was no correlation between resistance of Campylobacter sp strains to antimicrobials and the occurrence of diarrhea in calves. The frequency of resistance and, most importantly, multi drug resistance found among Campylobacter sp strains isolated from calves in Minas Gerais, Brazil, were high and the patterns of resistance observed are related to the antimicrobials agents most largely used in cattle in Brazil.Foi estudado o perfil de susceptibilidade aos antimicrobianos de 25 amostras de Campylobacter sp isoladas de bezerros com e sem diarréia (7 C. coli, 16 C. fetus e 2 C. jejuni. Foram testados pelo método de difusão 11 agentes antimicrobianos: amicacina, ampicilina, canamicina, cloranfenicol, eritromicina, gentamicina, neomicina, nitrofurantoína, penicilina G, tetraciclina e sulfametoxazole-trimetoprim. Todas as amostras de Campylobacter sp foram susceptíveis a amicacina, ampicilina, cloranfenicol, eritromicina

  12. Sobrevivência e perfil de resistência a antimicrobianos de Salmonella sp. isoladas em um sistema de tratamento de dejetos de suínos Survival and resistance patterns of Salmonella sp. isolated in a pig slurry treatment plant

    Directory of Open Access Journals (Sweden)

    Verônica Schmidt

    2003-10-01

    Full Text Available No presente estudo, foi avaliada a sobrevivência de Salmonella sp., presente em dejetos suínos, durante tratamento em um sistema de separação física e lagoas de estabilização ligadas em série. Nas amostras de Salmonella sp. isoladas foi determinado o perfil de resistência pelo método de difusão em ágar, usando 14 antimicrobianos. Das 20 coletas realizadas, foi possível isolar Salmonella sp. em 13 coletas no ponto correspondente ao início do sistema de tratamento e em apenas uma no ponto final do mesmo. Amostras de Salmonella sp. isoladas (161/163 pertenciam ao sorotipo Typhimurium e demonstraram resistência contra sulfonamida (100%, tetraciclina (99,4%, estreptomicina (90,1%, sulfa/trimetoprima (84,5%, ácido nalidíxico (77,6%, ampicilina (76,4%, cloranfenicol (29,2%, cefaclor (25,5%, tobramicina (13,7%, gentamicina (6,2%, amoxacilina/ácido clavulânico (5%, neomicina (5% e amicacina (3,7%. A maioria (94,5% das amostras isoladas foram resistente a 4 ou mais antimicrobianos e apresentaram grande variabilidade nos perfis de resistência. O nível de resistência e a variabilidade dos perfis mantiveram-se em nível semelhante ao longo do sistema.The survival of Salmonella sp. in pig slurry submitted to treatment in successive stabilization ponds on a pig-breeding farm was investigated. Furthermore, the isolated Salmonella strains were tested for their resistance against 14 antibiotics, using the agar diffusion method. Of a total of 20 samples taken from different points in the stabilization ponds system, 13 were positive for Salmonella sp. in the beginning and only one at the end of the system. Most of the isolated Salmonella strains (161/163 belonged to sorovar Typhimurium. These strains were resistant to sulfonamide (100%, tetracycline (9.4%, sulfamethoxazole/trimethoprin (84.5%, ampicillin (76.4%, cloramphenicol (29.2%, streptomycin (90.1%, nalidixic acid (77.6%, tobramycin (13.7%, neomycin (5%, amikacin (3.7%, cefaclor (25

  13. Kinerja probiotik Bacillus sp. pada pendederan benih ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila

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    , Sukenda

    2016-12-01

    Full Text Available ABSTRACT This experiment was conducted to assess performance of Bacillus sp. probiotic on catfish juvenile Clarias sp. infected by Aeromonas hydrophila. The probiotic content in the diets were 0% (K+ and K-, 1%, and 2% in duplicates. This experiment used randomized design with four treatments and two replications. Juveniles with average body weight of 3.22±0.15 g/fish were reared in the 1.5×2.8×0.5 m3 pond with density of 800 fish/pond. Fish were reared for 30 days and fed three times a day at rate 8% of  total body weight. At day 31, catfish were challenged by A. hydrophila 0.1 mL (106 cfu/mL. Post infection observation was carried out ten days with density 10 fish/aquaria. The result showed that fish fed diet containing 2% probiotic gave the best probiotic performance with survival rate of catfish 83.33% after challenged, spesific growth rate 5.40%, and 0,75 of feed conversion ratio. The results of the blood profile showed significantly better results in the treatment of probiotics compared to the positive control after challenge test A. hydrophila. Probiotic Bacillus sp. has given as much as 2% on feed provides better performance on catfish juvenile. Keywords: probiotic, Bacillus sp., A. hydrophila, catfish juvenille, growth  ABSTRAK Penelitian ini bertujuan untuk menguji kinerja probiotik Bacillus sp. dalam pakan pada pendederan benih ikan lele Clarias sp. yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu kandungan probiotik dalam pakan perlakuan yaitu 0% (K+ dan K-, 1%,  dan 2%, masing-masing dengan dua ulangan. Ikan lele yang digunakan memiliki bobot rata-rata 3,22±0,15 g/ekor, dipelihara dalam kolam terpal berukuran 1,5×2,8×0,5 m3 dengan kepadatan 800 ekor/kolam. Ikan dipelihara selama 30 hari dengan frekuensi pemberian pakan tiga kali sehari sebanyak 8% dari bobot tubuh ikan. Hari ke-31 benih lele diinjeksi bakteri A. hydrophila dosis 0,1 m

  14. Control of Branchionus sp. and Amoeba sp. in cultures of Arthrospira sp. Control de Branchionus sp. y Amoeba sp. en cultivos de Arthrospira sp.

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    Carlos Méndez

    2012-09-01

    Full Text Available Cultivation of cyanobacterium Arthrospira sp. has been developed in many countries for the production of proteins, pigments and other compounds. Outdoor mass cultures are often affected by biological contamination, drastically reducing productivity as far as bringing death. This study evaluates the control of Branchionus sp. and Amoeba sp. with two chemical compounds: urea (U and ammonium bicarbonate (AB, in laboratory conditions and outdoor mass culture of Arthrospira sp. The lethal concentration 100 (LC100 at 24 h for Branchionus sp. and Amoeba sp. determined was of 60-80 mg L-1 (U and 100-150 mg L-1 (AB. The average effective inhibition concentration for 50% of the population (IC50 in Arthrospira sp., after 72 h, was 80 mg L-1 (U and 150 mg L-1 (AB. The application of doses of 60 mg L-1 (U or 100 mg L-1 (AB in the outdoor mass culture of this contaminated microalga, completely inhibited grazing and did not affect the growth of Arthrospira sp. but rather promoted rapid recovery of algal density at levels prior to infestation. These compounds provided an economical and effective control of predators in cultures of Arthrospira sp.El cultivo de la cianobacteria Arthrospira sp. ha sido desarrollado en muchos países para la obtención de proteínas, pigmentos y otros compuestos. Cultivo que a nivel industrial se ve afectado frecuentemente por contaminación biológica, reduciendo drásticamente la productividad hasta causar la muerte. Este estudio evalúa el control de Branchionus sp. y de Amoeba sp. con dos compuestos químicos, la urea (U y bicarbonato de amonio (AB en cultivos de Arthrospira sp. La concentración letal 100 (LC100 determinada a las 24 h para Branchionus sp. y Amoeba sp. fue de 60-80 mg L-1 (U y 100-150 mg L-1 (AB. La concentración media de inhibición efectiva, después de 72 h, para el 50% de la población (IC50 en Arthrospira fue de 80 mg L-1 (U y 150 mg L-1 (AB. La aplicación de dosis de 60 mg L-1 (U ó 100 mg L-1 (AB en

  15. A mortalidade neonatal em 1998, no município de Botucatu - SP La mortalidad neonatal en 1998, en el município de Botucatu-SP The neonatal mortality in 1998 at the municipality of Botucatu-SP

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    Kátia Poles

    2000-07-01

    Full Text Available Considerando que a mortalidade neonatal é indicador da qualidade da assistência prestada à gestante, ao parto e ao recém-nascido, realizamos o presente trabalho, cujo objetivo foi identificar as causas e o índice de mortalidade neonatal durante o ano de 1998 em Botucatu-SP. O coeficiente de mortalidade neonatal obtido foi de 8,3/1000 nascidos vivos e o coeficiente de mortalidade neonatal precoce foi de 7,3/1000 nascidos vivos, confirmando a importância dos óbitos na primeira semana de vida. Aproximadamente três quartos dos óbitos puderam ser classificados como reduzíveis por diagnóstico e tratamento precoces, reduzíveis por adequada atenção ao parto ou parcialmente reduzíveis por adequado controle da gravidez, evidenciando que para se reduzir os índices de morte neonatal, deveremos investir na melhoria da qualidade da assistência prestada à gestante, à parturiente e ao neonato.Considerando que la mortalidad neonatal es un indicador de la calidad de la asistencia ofrecida en la gestación, al parto y al recién- nacido, realizamos el presente trabajo, con el objetivo de estudiar la mortalidad neonatal durante el año de 1998 en Botucatu-SP. El coeficiente de mortalidad neonatal encontrado fue 8,3/1000 nacidos vivos y el coeficiente de mortalidad neonatal precoz fue de 7,3/1000 nacidos vivos, confirmando la importancia de las defunciones en la primera semana de vida. Los resultados mostraron que aproximadamente tres cuartos de las defunciones pueden ser reducidas con el control del embarazo, con el diagnóstico y tratamiento precoz o con la adecuada atención al parto, evidenciando que para reducir los índices de mortalidad neonatal, deberemos invertir en la mejoría de la calidad de la asistencia ofrecida en la gestación, en el parto y al recién-nacido.Considering that neonatal mortality is an indicator of the quality of the care provided to pregnant women, at childbirth as well as to the new born, authors developed the

  16. Altered Bacterial Profiles in Saliva from Adults with Caries Lesions

    DEFF Research Database (Denmark)

    Belstrøm, D; Fiehn, N-E; Nielsen, C H

    2014-01-01

    -Whitney's test with Benjamini-Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles. A reduced bacterial diversity was observed in samples from subjects with dental caries. Five bacterial taxa (Veillonella parvula, Veillonella atypica......, Megasphaera micronuciformis, Fusobacterium periodontium and Achromobacter xylosoxidans) and one bacterial cluster (Leptotrichia sp. clones C3MKM102 and GT018_ot417/462) were less frequently found in the caries group (adjusted p value ... salivarius) and three bacterial clusters (Streptococcus parasanguinis I and II and sp. clone BE024_ot057/411/721, Streptococcus parasanguinis I and II and sinensis_ot411/721/767, Streptococcus salivarius and sp. clone FO042_ot067/755) were present at significantly higher levels (adjusted p value

  17. A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c

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    Wanarska Marta

    2012-08-01

    Full Text Available Abstract Background D-Tagatose is a natural monosaccharide which can be used as a low-calorie sugar substitute in food, beverages and pharmaceutical products. It is also currently being tested as an anti-diabetic and obesity control drug. D-Tagatose is a rare sugar, but it can be manufactured by the chemical or enzymatic isomerization of D-galactose obtained by a β-D-galactosidase-catalyzed hydrolysis of milk sugar lactose and the separation of D-glucose and D-galactose. L-Arabinose isomerases catalyze in vitro the conversion of D-galactose to D-tagatose and are the most promising enzymes for the large-scale production of D-tagatose. Results In this study, the araA gene from psychrotolerant Antarctic bacterium Arthrobacter sp. 22c was isolated, cloned and expressed in Escherichia coli. The active form of recombinant Arthrobacter sp. 22c L-arabinose isomerase consists of six subunits with a combined molecular weight of approximately 335 kDa. The maximum activity of this enzyme towards D-galactose was determined as occurring at 52°C; however, it exhibited over 60% of maximum activity at 30°C. The recombinant Arthrobacter sp. 22c L-arabinose isomerase was optimally active at a broad pH range of 5 to 9. This enzyme is not dependent on divalent metal ions, since it was only marginally activated by Mg2+, Mn2+ or Ca2+ and slightly inhibited by Co2+ or Ni2+. The bioconversion yield of D-galactose to D-tagatose by the purified L-arabinose isomerase reached 30% after 36 h at 50°C. In this study, a recombinant Pichia pastoris yeast strain secreting β-D-galactosidase Arthrobacter chlorophenolicus was also constructed. During cultivation of this strain in a whey permeate, lactose was hydrolyzed and D-glucose was metabolized, whereas D-galactose was accumulated in the medium. Moreover, cultivation of the P. pastoris strain secreting β-D-galactosidase in a whey permeate supplemented with Arthrobacter sp. 22c L-arabinose isomerase resulted in a 90% yield

  18. A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c.

    Science.gov (United States)

    Wanarska, Marta; Kur, Józef

    2012-08-23

    D-Tagatose is a natural monosaccharide which can be used as a low-calorie sugar substitute in food, beverages and pharmaceutical products. It is also currently being tested as an anti-diabetic and obesity control drug. D-Tagatose is a rare sugar, but it can be manufactured by the chemical or enzymatic isomerization of D-galactose obtained by a β-D-galactosidase-catalyzed hydrolysis of milk sugar lactose and the separation of D-glucose and D-galactose. L-Arabinose isomerases catalyze in vitro the conversion of D-galactose to D-tagatose and are the most promising enzymes for the large-scale production of D-tagatose. In this study, the araA gene from psychrotolerant Antarctic bacterium Arthrobacter sp. 22c was isolated, cloned and expressed in Escherichia coli. The active form of recombinant Arthrobacter sp. 22c L-arabinose isomerase consists of six subunits with a combined molecular weight of approximately 335 kDa. The maximum activity of this enzyme towards D-galactose was determined as occurring at 52°C; however, it exhibited over 60% of maximum activity at 30°C. The recombinant Arthrobacter sp. 22c L-arabinose isomerase was optimally active at a broad pH range of 5 to 9. This enzyme is not dependent on divalent metal ions, since it was only marginally activated by Mg2+, Mn2+ or Ca2+ and slightly inhibited by Co2+ or Ni2+. The bioconversion yield of D-galactose to D-tagatose by the purified L-arabinose isomerase reached 30% after 36 h at 50°C. In this study, a recombinant Pichia pastoris yeast strain secreting β-D-galactosidase Arthrobacter chlorophenolicus was also constructed. During cultivation of this strain in a whey permeate, lactose was hydrolyzed and D-glucose was metabolized, whereas D-galactose was accumulated in the medium. Moreover, cultivation of the P. pastoris strain secreting β-D-galactosidase in a whey permeate supplemented with Arthrobacter sp. 22c L-arabinose isomerase resulted in a 90% yield of lactose hydrolysis, the complete utilization

  19. SELEKSI DAN PEMANFAATAN ACTINOMYCETES SEBAGAI MIKROBA ANTAGONIS YANG RAMAH LINGKUNGAN TERHADAP Fusarium oxysporum f.sp. cubense SECARA IN VITRO

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    I MADE SUDARMA

    2015-06-01

    Full Text Available A total of 119 different actinomycete isolate were recovered from banana crop habitats with and without Fusarium wilt disease symptom. These were than assessed for their antagonist ability against Fusarium oxysporum £sp. cubense (Foe in vitro. Results indicated that four of all actinomycete isolate active against Foe. The four of actinomycete isolates were Streptomyces sp. l (AAo4, Streptomyces sp.2 (AAo32 , Streptomyces sp.3 (AAo33 and Streptomyces sp. 4 (AAo35. It was can inhibit the Foe mycelium growth, 79,63%, 72,22%, 78,89% and 72,22% respectively. After tested with the 3 times replication, the four Streptomyces spp. isolate effective to control the Foe that attack Bali banana cultivars, such as Susu, Saba, Raja and Ketip.

  20. Eiseniicola composti gen. nov., sp. nov., with antifungal activity against plant pathogenic fungi.

    Science.gov (United States)

    Yasir, Muhammad; Aslam, Zubair; Song, Geun Cheol; Jeon, Che Ok; Chung, Young Ryun

    2010-01-01

    A Gram-negative, short rod-shaped bacterial strain, YC06271T, was isolated from the vermicompost (VC) collected at Masan, Korea and its taxonomic position was investigated by a polyphasic taxonomic approach. Strain YC06271T grew optimally at 28-30 degrees C and at pH 7.0-9.0. The 16S rRNA gene sequence of strain YC06271T was most closely related to members of the genera Bordetella (96.4-95.8 %), Achromobacter (96.0-95.7 %), Alcaligenes (96.0-94.2 %), Pusillimonas noertemannii (95.9 %), Pigmentiphaga (95.8-95.5 %) and less than 95.5 % similarity with the members of the other genera of the family Alcaligenaceae. Strain YC06271T contained ubiquinone-8 (Q-8) as the major respiratory quinone system and putrescine as the major polyamine. The major fatty acids of strain YC06271T were C16:1omega7c and/or C15:0 iso 2-OH and C16:0. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 55.4 mol%. Phylogenetic analysis, biochemical, chemotaxonomic and phenotypic characteristics strongly supported the differentiation of strain YC06271T from the validly published genera of the family Alcaligenaceae. Therefore, it is proposed that strain YC06271T represents a novel species within a novel genus, with the name Eiseniicola composti gen. nov., sp. nov. The type strain is YC06271T (= KCTC 22250T = DSM 21045T).

  1. Experiências de médicos ao comunicarem o diagnóstico da deficiência de bebês aos pais = Physicians’ experiences when communicating the diagnosis of infants’ disability to parents

    Directory of Open Access Journals (Sweden)

    Luisada, Valquíria

    2015-01-01

    Full Text Available Objetivo: O presente estudo teve como objetivo investigar os sentimentos e vivências de médicos ao transmitirem a notícia de uma deficiência aos pais, no nascimento do bebê, assim como o preparo/formação que receberam para atuarem neste momento. Material e Métodos: O estudo teve abordagem qualitativa e exploratória, a partir da técnica de entrevista semiestruturada. A pesquisa foi realizada com 10 médicos, entre Obstetras e Neonatologistas na cidade de São Paulo/ SP. As entrevistas foram analisadas e seus resultados discutidos em três diferentes categorias: a Notícia, Sentimentos e Preparo Profissional. Resultados: Os médicos relatam dificuldades ao comunicar os pais sobre a deficiência dos bebês e pensam em estratégias variadas, como falar ao casal; falar apenas para a mãe, falar imediatamente após o parto, esperar algum tempo; revelam tristeza e inexistência de preparo durante a formação em medicina. Conclusão: Foram sinalizadas abordagens inadequadas e ineficazes, e o despreparo e dificuldades na comunicação da notícia, associados à falta de formação acadêmica para desempenhar esta tarefa. Conclui-se que os principais sentimentos vivenciados são a tristeza, a angústia e a solidariedade, com consequências físicas e emocionais para o profissional e, provavelmente, para a família dos pacientes

  2. Respiratory infections by Achromobacter xylosoxidans in a cohort of Cystic Fibrosis patients: identification, antimicrobial susceptibility and molecular epidemiology

    Directory of Open Access Journals (Sweden)

    Antonietta Lambiase

    2010-03-01

    Full Text Available Pulmonary infections by Gram-negative bacteria such as Achromobacter xylosoxidans are recovered frequently in patients with Cystic Fibrosis. Aims of this study were to value the isolation frequency of A.xylosoxidans strains in a cohort of Cystic Fibrosis patients, to investigate their antimicrobial sensitivity and to establish possible clonal likeness among strains.A retrospective study was undertaken between January 2004 and December 2008 on 300 patients receiving care at the Regional Cystic Fibrosis Centre of “Federico II” University, Naples. Sputum samples were collected and selective media as well as commercial systems for bacterial identification were used. The activity of antimicrobial agents was determined using diffusion and micro-dilution methods. For DNA-fingerprinting, a genomic DNA macrorestriction followed by pulsed-field gel electrophoresis was carried out. A total of 238 strains from 51 patients were isolated. Strains were resistant to aztreonam, about half of these were resistant to gentamicin and trimethoprim-sulphamethoxazole. They were sensitive to piperacillin, piperacillin/tazobactam, and also to carbapenems, quinolones, cephalosporines. Macrorestriction analysis applied on some isolates showed substantial heterogeneity among strains.Actually, the prognostic role of A. xylosoxidans in Cystic Fibrosis is unclear, but this finding must imply difficulties on therapeutic approach. So, it is need to be on the look out regard such microorganisms. Preliminary results of DNA-fingerprinting indicate no evidence of clonal likeness and then of patient-to-patient spread.

  3. O Outro na Cidade: Deficiência, Acessibilidade e Saúde em Presidente Prudente-SP | The Other City: Disability, Accessibility and Health en Presidente Prudente-SP

    Directory of Open Access Journals (Sweden)

    Natália Cristina Alves

    2014-05-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE Introdução: O acesso aos serviços de saúde pode ser visto como uma forma de compreensão dos processos de produção e reprodução dos espaços urbanos contemporâneos, especialmente, por que o uso e apropriação da cidade podem revelar inúmeras formas de exclusão e desigualdade social. Tal situação é ainda mais perceptível quando se trata de pessoas com algum tipo de deficiência, que precisam se locomover em busca da saúde pelo espaço urbano. O objetivo desta pesquisa é entender como se concretiza a mobilidade e a acessibilidade do portador de deficiência ao sistema de saúde de Presidente Prudente-SP, de forma a captar as estratégias e os contextos geográficos desses sujeitos. Para tanto, pretendemos discutir e analisar a deficiência do ponto de vista geográfico, além de basear o trabalho através da pesquisa qualitativa a respeito do acesso e acessibilidade tomando como referência a vida cotidiana das pessoas com deficiência(s moradoras de Presidente Prudente-SP. Ao fazermos este recorte temático, pretendemos demonstrar, portanto, que é possível uma compreensão mais clara das desigualdades de saúde-doença no espaço urbano, uma vez que os sujeitos representam e concebem tal espaço, de forma singular. Metodologia/Desenvolvimento: Publicações do Instituto de Geografia e Estatística (IBGE, com dados preliminares da amostra do Censo 2010, revelam que o Brasil conta com um índice de 23,9% de pessoas com deficiência, aproximadamente 45,6 milhões de pessoas. Em Presidente Prudente-SP, o número da população residente que possui alguma deficiência visual, auditiva, motora ou mental/intelectual investigadas, é de 21.4% de seus 207.610 habitantes. Ao fazer o mapeamento das deficiências para Presidente Prudente-SP, constata-se a existência de um maior contingente desta população nas áreas periféricas e longe dos estabelecimentos de saúde da cidade. Tais

  4. Isozyme-based genetic fingerprinting of Manihot sp

    African Journals Online (AJOL)

    Prof. Ogunji

    1973-06-22

    Jun 22, 1973 ... Isozyme-based genetic fingerprinting of Manihot sp. Efisue, A. A.. Development of Crop & Soil Science, University of Port Harcourt P.M.B. 5323 Choba, Port Harcourt,. Rivers State, Nigeria. (Received 29:10:13, Accepted 20:12:13). Abstract. Many cassava varieties have been released into farmers' fields in ...

  5. ESSAY ON Elasmopalpus lignosellus (ZELLER, 1848 AND TO Syntermes sp., Procornitermes spp., AND Cornitermes spp. CONTROL IN MAIZE BY SEED TREATMENT ENSAIO DE CONTROLE À LAGARTA DO COLO Elasmopaplus lignosellus (Zeller, 1984 E AOS CUPINS Syntermes sp., Procornitermes spp. E Cornitermes spp., NA CULTURA DO MILHO, VIA TRATAMENTO DE SEMENTES

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    Luiz Carlos Corrêa de Oliveira Júnior

    2007-09-01

    Full Text Available

    This study presents the results of the field experiments carried out in Goiânia, Goiás State, Brazil, in 1985/86, to estimate the efficiency of insecticides in controlling the Lesser Cornstalk (Elasmopaplus lignosellus and the termites Syntermes sp., Procornitermes spp. and Cornitermes spp. which attack corn plants, by seed treatment. The results show that benfuracarbe, carbofuran and thiodicarbe, respectively, were highly efficient in controlling both pests, lesser cornstalk borer and termites up to 21 days after seed germination.

    Foi realizado um experimento com milho no município de Goiânia, Goiás, safra 1985/86, visando ao controle da lagarta do colo Elasmopaplus lignosellus e cupins  Syntermes sp., Procornitermes spp. e Cornitermes spp., via tratamento de sementes. Pelos resultados obtidos concluiu-se que Thiodicarbe na dose de 700 g. i.a. (gramas de ingrediente ativo; Carbofuran na dose de 700 g. i.a. e Benfuracarbe nas doses de 800 e 1000 g. i.a. / 100 kg de sementes deram 84,3 , 97,1 e 96,5% de controle a E. lignosellus, respectivamente, e controlaram 82,7 , 100,0 e 96,0% de cupins, na mesma ordem, até 21 dias após germinação das sementes.

  6. Características laboratoriais das ceratites e conjuntivites causadas por Streptococcus sp Laboratorial findings of Streptococcus keratitis and conjunctivitis

    Directory of Open Access Journals (Sweden)

    Helena Parente Solari

    2004-10-01

    Full Text Available OBJETIVOS: Analisar os resultados laboratoriais de conjuntivites e ceratites com cultura positiva para Streptococcus sp, avaliando a incidência das diferentes espécies e os dados dos antibiogramas. MÉTODOS: Estudo retrospectivo de revisão de prontuários de pacientes encaminhados ao laboratório de Doenças Externas do Departamento de Oftalmologia da UNIFESP com resultado de cultivo bacteriano positivo de córnea ou conjuntiva e com identificação de alguma cepa do gênero Streptococcus sp, no período de janeiro de 1995 a dezembro de 2001. Analisou-se idade do paciente, espécie de Streptococcus e os testes de sensibilidade aos seguintes antibióticos: cefalotina, amicacina, gentamicina, tobramicina, ciprofloxacina, lomefloxacina, ofloxacina, norfloxacina e vancomicina. RESULTADOS: As espécies mais encontradas foram Streptococcus pneumoniae e Streptococcus viridans. Com relação aos antibióticos, a sensibilidade foi maior à cefalotina, às quinolonas e à vancomicina. CONCLUSÕES: Considerando-se os antibióticos tópicos comercialmente disponíveis, as quinolonas apresentam melhor espectro de ação quando comparadas aos aminoglicosídios.PURPOSE: To evaluate laboratorial findings of Streptococcus keratitis and conjunctivitis, analyzing the different species and the results of bacterial susceptibility to an antibiotics. METHODS: Retrospective study of the records from the External Disease Laboratory of the Ophthalmology Department of the Federal University of São Paulo, with conjunctival or corneal positive bacterial culture for Streptococcus sp, between January 1995 and December 2001. The collected data were age, Streptococcus species and the bacterial susceptibility to the following antibiotics: cephalotin, amikacin, gentamicin, tobramicin, ciprofloxacin, lomefloxacin, ofloxacin, norfloxacin and vancomicin. RESULTS: The most frequent species were Streptococcus pneumoniae and Streptococcus viridans. Regarding bacterial

  7. ENTOMOFAUNA ASSOCIADA AOS FRUTOS DO BACUPARI, Salacia folia (MART. PEYR, NOS CERRADOS DO BRASIL CENTRAL INSECTS ASSOCIED WITH BACUPARI FRUIT, Salacia crassifolia (Mart. PEYR, IN THE SAVANNAHS OF CENTRAL BRAZIL

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    Juracy Rocha Braga Filho

    2007-09-01

    Full Text Available

    O bacupari, Salacia crassifolia (Mart. Peyr, (Hippocrateaceae é uma frutífera nativa da região dos cerrados, utilizada pela fauna e também pela população da região, embora seus frutos sejam muito atacados pelas moscas-das-frutas, e por isso seu consumo fica limitado. De polpa branca à alaranjada, de sabor levemente ácido e muito agradável os frutos dessa espécie podem ser consumidos ao natural ou na forma de sucos. Neste trabalho apresenta-se um levantamento da entomofauna associada aos frutos do bacupari, coletados em 11 municípios da região dos cerrados no Estado de Goiás, no período de setembro de 1995 a dezembro de 1999. No Laboratório de Entomologia da Escola de Agronomia da Universidade Federal de Goiás, esses frutos foram, inicialmente, contados e pesados e, posteriormente, acondicionados em caixas de polipropileno com areia úmida para obtenção de pupários e insetos adultos. Tephritidae foi a família mais freqüente nos frutos do bacupari, com 54,22% dos insetos coletados. Nessa família foram registradas as espécies Anastrepha sp. (espécie nova em fase de descrição, A. zenildae, A. obliqua, A. fraterculus, A. sororcula e A. serpentina. Na família Lonchaeidae foi coletada Neosilba sp., em Muscidae, Atherigona orientalis e espécimes da família Oititidae. Dessas moscas foram obtidos os parasitóides Doryctobracon sp. (espécie nova, D. areolatus, Dicerataspis flavipes, Lopheucoila anastrephae, Aganaspis nordlanderi e A. pelleranoi. Foram coletados isoladamente no mesmo fruto exemplares de Anastrepha sp. e Lonchaeidae e Anastrepha sp. e A. zenildae

  8. Diaspidídeos e parasitoides associados ao cultivo da videira no Rio Grande do Sul, Brasil

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    Vera Regina dos Santos Wolff

    2014-12-01

    Full Text Available As cochonilhas-com-escudo (Hemiptera, Diaspididae têm causado prejuízos significativos à cultura da videira no Rio Grande do Sul, principalmente em Vitis labrusca L. da cv. Niágara. No período de setembro de 2009 a outubro de 2011, foi realizado um inventário das espécies de Diaspididae ocorrentes no tronco das videiras e dos parasitoides associados. Foram avaliadas 32 propriedades localizadas nos municípios de Bento Gonçalves, Caxias do Sul, Flores da Cunha e Sarandi-RS. A espécie mais frequente e abundante foi Hemiberlesia lataniae (Signoret, 1869. Melanaspis arnaldoi (Costa Lima, 1924 e Pseudaonidia marquesi Costa Lima, 1924 foram registradas pela primeira vez em V. labrusca. Todas as espécies de cochonilhas apresentaram perfurações nos escudos indicando níveis de parasitismo natural que variaram de 11,3 a 76,5% para H. lataniae, de 9 a 58,8% para M. arnaldoi e de 3,4 a 22,2% para P. marquesi. As seguintes associações entre parasitoides e cochonilhas foram encontradas: Encarsia sp. (Aphelinidae, Plagiomerus sp. (Encyrtidae, Amitus sp. (Platygastridae e o hiperparasitoide Chartocerus sp. (Signiphoridae a H. lataniae; Homalopoda sp. (Encyrtidae a P. marquesi e Chartocerus sp. a M. arnaldoi. Amitus sp., Homalopoda sp. e Chartocerus sp. foram associados pela primeira vez com estes diaspidídeos.

  9. Morbidades referidas por trabalhadoras que produzem joias folheadas em Limeira, SP

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    Vanessa Catherina Neumann Figueiredo

    Full Text Available RESUMO INTRODUÇÃO: a cidade de Limeira (SP se caracteriza por ser um polo de empresas do ramo de joias folheadas e bijuterias, sendo a maior parte da mão de obra composta por mulheres. OBJETIVO: verificar as morbidades referidas por mulheres expostas ao cianeto no setor de bijuterias e joias folheadas na cidade de Limeira em 2007. METEDOLOGIA: estudo transversal realizado com 383 trabalhadoras, comparando-se um grupo de 191 mulheres trabalhadoras em galvanoplastias consideradas qualitativamente expostas ao cianeto com um grupo de 192 mulheres não expostas ao cianeto. Foi aplicado o Índice de Capacidade para o Trabalho, o Questionário de Tolerância de Fagerström e um questionário sociodemográfico e ocupacional. RESULTADOS: O grupo considerado exposto ao cianeto apresentou: menor média de tempo de trabalho no cargo (p = 0,008, maior proporção que trabalha com produtos químicos (p < 0,001, que usa Equipamento de Proteção Individual (p < 0,001, que recebe equipamento das empresas em que trabalham (p < 0,001, menor quantidade de morbidades referidas (p = 0,042, mas maior prevalência de bronquite crônica (p = 0,027, sinusite crônica (p = 0,022 e disfunções da tireoide (p = 0,022. CONCLUSÕES: os resultados apontam para a necessidade de pesquisas de coorte que analisem a associação entre os agravos apresentados e a exposição ao cianeto.

  10. Características estruturais e morfológicas de genótipos de Pennisetum sp. sob pastejo no período de seca Structural and morphological characteristics of Pennisetum sp. genotypes under grazing during the dry period

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    Márcio Vieira da Cunha

    2007-06-01

    Full Text Available O experimento foi realizado com o objetivo de estudar características estruturais e morfológicas de cinco genótipos de Pennisetum sp. (CE 08 A.D., Venezuela, HV-241, Elefante B e Hexaplóide sob pastejo no período de seca na Zona da Mata de Pernambuco. Os genótipos foram manejados sob lotação rotacionada (44 dias de descanso e quatro dias de pastejo ao longo de dois ciclos de pastejo. Os dados foram analisados em delineamento experimental de blocos ao acaso com parcelas subdivididas (os genótipos representaram as parcelas e os ciclos de pastejo, as subparcelas e quatro repetições. Houve variação nas características estruturais e morfológicas de genótipos de Pennisetum sp. Os genótipos CE 08 A.D. e Elefante B apresentaram maior densidade de lâmina foliar verde, menor porcentagem de material morto e maior densidade de perfilhos basais remanescentes e aéreos novos, com médias de 5,0±0,4 kg de MS/ha/cm, 33,0±0,2%, 22,0±0,2 e 146,0±0,4 perfilhos/m², respectivamente. O HV-241 apresentou alta participação de material morto em sua biomassa aérea (55,6±0,3%, o que pode ser atribuído à alta mortalidade de perfilhos (19,0±0,3 e 114,0±0,4 perfilhos basais e aéreos mortos/m², respectivamente. Os genótipos CE 08 A.D. e Elefante B destacaram-se como promissores para utilização sob pastejo no período de seca. O genótipo HV-241, híbrido de capim-elefante com o milheto, foi mais afetado pelos efeitos do estresse hídrico no período seco do ano.The experiment was carried out to study structural and morphologic characteristics of five Pennisetum sp. genotypes (CE 08 A.D., Venezuela, HV-241, Elephant B and Hexaplóide under grazing during the dry period in Pernambuco Forest Zone. Genotypes were managed under rotational stocking (44 days of resting and four days of grazing period. The experimental design was randomized blocks in a split plot arrangement, and four replications. The genotypes represented the plots, and grazing

  11. Radioimmunoassay of serum SP 1 and HPL in normal and abnormal pregnancies

    International Nuclear Information System (INIS)

    Pluta, M.; Hardt, W.; Schmidt-Gollwitzer, K.; Schmidt-Gollwitzer, M.

    1979-01-01

    Serum concentrations of the pregnancy-specific β 1 -glycoprotein (SP 1) and human placental lactogen (HPL) were measured by radioimmunoassay in 372 blood samples obtained from 40 women in the second half of a normal singleton pregnancy. The mean level of SP 1 steadily increased from 40 μg/ml in the 22nd week of pregnancy to 168 μg/ml in the 36th week of gestation and thereafter reached a plateau. The half-life of SP 1 during the first week after delivery was about 39 h. The clinical value of SP 1 in comparison to HPL estimation was assessed in a prospective study of a few high risk pregnancies. There were no significant differences between serum SP 1 and HPL levels in pregnancies complicated by preeclampsia with or without intrauterine growth retardation and in twin pregnancies. Serum HPL and SP 1 levels were equally effective in predicting placental insufficiency with fetal growth retardation. (orig./AJ) [de

  12. Morfologia da glândula pineal de gambás (Didelphis sp

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    Celina Almeida Furlanetto Mançanares

    2007-06-01

    Full Text Available A glândula pineal deve ser analisada e estudada em animais da fauna brasileira, para que dados da pesquisa básica possam ser aplicados em novas técnicas de manejo reprodutivo destes animais, inclusive em cativeiro, face à íntima relação deste órgão fotorreceptor com o ciclo reprodutivo. Para este estudo, foram utilizados 10 gambás (Didelphis sp, provenientes do Departamento de Anatomia da USP e da UNIFEOB, já mortos e fixados. Nenhum animal foi submetido a situações de dor/sofrimento e ao sacrifício de sua vida. A glândula pineal foi encontrada em todos animais estudados e apresentou-se com diminutas dimensões, não sendo possível, portanto descrever-lhe características macroscópicas. Através da análise microscópica pudemos localizar a glândula no espaço correspondente ao plano mediano, em relação ao encéfalo, rostral e dorsalmente aos colículos rostrais, ventralmente aos hemisférios cerebrais e caudalmente à comissura habenular. Consiste de uma evaginação do teto do diencéfalo e mostra-se em forma de "U" invertido. Comparativamente a características de glândulas pineais de outras espécies animais, a do Didelphis genus, que estudamos, revela peculiaridades tanto em relação ao seu tamanho, apenas perceptível microscopicamente, quanto ao fato de apresentar células semelhantes às secretoras, dispersas também em áreas vizinhas. Tais peculiaridades motivam reflexões sobre o papel funcional da glândula, na espécie considerada.

  13. Resistência de genótipos de caupi ao caruncho

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    BARRETO PAULO DIÓGENES

    2000-01-01

    Full Text Available A utilização de resistência genética ao ataque de Callosobruchus maculatus (Fabr. tem sido alvo de investigação científica, especialmente no que diz respeito à identificação de fontes de resistência. O presente trabalho objetivou incorporar, ao grupo de caracteres desejáveis para o cultivo de caupi (Vigna unguiculata (L. Walp., resistência genética ao caruncho (C. maculatus. Foram realizadas hibridações dos genótipos IT81D-1045 e IT81D-1064 (portadores de resistência ao inseto com CNCx 252-1E/FB, CNCx 187-22D-1 e BR 1-Poty (capazes de transferir resistência a viroses, tolerância à seca, formação de grãos com padrão comercial, elevado potencial de produção e adaptabilidade a diferentes condições ambientais. Populações segregantes obtidas destes cruzamentos foram conduzidas pelo método SPD (descendência de uma única vagem, e na geração F5 foram realizadas seleções individuais. As linhagens obtidas foram avaliadas em conjunto com materiais de origens diferentes, utilizando-se parâmetros associados à infestação da praga. Foi constatado que os genótipos avaliados apresentaram variabilidade quanto à preferência à postura, número de insetos emergidos e número de sementes danificadas; as linhas EVx 37-15E e EVx 37-2E foram as que sofreram menor dano causado pelo caruncho; as variáveis número de ovos, número de insetos emergidos e número de sementes danificadas mostraram-se positiva e significativamente correlacionadas entre si; o grupo das linhagens que descendem de genitores resistentes apresenta valores significativamente inferiores aos obtidos pelas demais, o que indica que a resistência ao inseto se transmite geneticamente.

  14. Novas citações de Chytridiomycota e Oomycota para o Parque Estadual das Fontes do Ipiranga (PEFI, SP, Brasil New records of Chytridiomycota and Oomycota from the "Parque Estadual das Fontes do Ipiranga (PEFI", SP, Brazil

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    Carmen Lidia Amorim Pires-Zottarelli

    2007-03-01

    Full Text Available Levantamento de fungos zoospóricos realizado em lagos artificiais do Parque Estadual das Fontes do Ipiranga (PEFI, situado na cidade de São Paulo, Estado de São Paulo, Brasil, resultou no isolamento de 41 táxons, sendo 36 identificados em nível específico. Vinte e três táxons pertencem ao Reino Fungi, filo Chytridiomycota, ordens Chytridiales, Monoblepharidales e Spizellomicetales e, 18 ao Reino Straminipila, filo Oomycota, ordens Saprolegniales e Peronosporales. Dentre os fungos isolados, 14 são mencionados pela primeira vez para o PEFI: Aphanomyces helicoides von Minden, Catenochytridium kevorkianii Sparrow, Catenochytridium sp., Chytriomyces appendiculatus Karling, C. aureus Karling, C. hyalinus Karling, C. spinosus Fay, Diplophlyctis asteroidea Dogma, D. complicata (Willoughby Dogma, Karlingia dubia Karling, Nowakowskiella hemisphaerospora Shanor, Saprolegnia australis Elliott, Septochytrium variabile Berdan e Truittella sp., os quais são descritos, comentados e ilustrados. O gênero Truitella é mencionado pela primeira vez para o Brasil.A survey of zoosporic fungi from artificial lakes of the Fontes do Ipiranga State Park, in the city of São Paulo, São Paulo State, Brazil, yielded the isolation of 41 taxa with 36 species. Twenty-three taxa belong to Chytridiales, Monoblepharidales and Spizellomycetales of the Phylum Chytridiomycota, Fungi Kingdom and 18 belong to Saprolegniales and Peronosporales, Phylum Oomycota, Straminipila Kingdom. Of these, 14 are first-time records for this State Park: Aphanomyces helicoides von Minden, Catenochytridium kevorkianii Sparrow, Catenochytridium sp., Chytriomyces appendiculatus Karling, C. aureus Karling, C. hyalinus Karling, C. spinosus Fay, Diplophlyctis asteroidea Dogma, D. complicata (Willoughby Dogma, Karlingia dubia Karling, Nowakowskiella hemisphaerospora Shanor, Saprolegnia australis Elliott, Septochytrium variabile Berdan and Truittella sp., which are described, commented and

  15. O estudante de Medicina e o médico recém-formado frente à morte e ao morrer

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    Gustavo Nader Marta

    Full Text Available A compreensão do papel da morte como uma condição intrínseca e inegável da vida sempre foi de grande relevância para o mundo ocidental, levando a diferentes atitudes, especialmente entre aqueles que precisam encarar a morte como parte de sua rotina profissional. Isto é particularmente aplicável aos médicos, que, muitas vezes, se acham diante da responsabilidade de tomar decisões sobre as condições de saúde e a vida de seus pacientes. Os objetivos deste estudo foram verificar o comportamento do estudante de Medicina e do médico recém-formado diante da morte e do morrer, identificar as condições e deficiências da relação médico-paciente-morrer-morte, comparar tais posturas entre os estudantes de Medicina e os médicos recém-formados, e propor atividades que possibilitem a sensibilização quanto aos processos internos com base em teoria, prática e desenvolvimento pessoal. Isso foi feito por meio da aplicação de questionários aos 100 alunos do terceiro ano e aos 120 residentes do CCMB-PUC-SP.

  16. Heparinoides naturais isolados de rodofíceas (Halymenia sp. arribadas na costa cearense =Natural heparinoids isolated from Halymenia sp. (Rhodophyceaes delivery on the Ceará coast

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    José Ariévilo Gurgel Rodrigues

    2010-07-01

    Full Text Available A crescente carência de heparina (HEP motiva a busca por fontes alternativas de novos anticoagulantes naturais. Objetivou-se avaliar a atividade anticoagulante dos polissacarídeos sulfatados (PS isolados de uma rodofícea do gênero Halymenia, nativa do litoral cearense, Brasil. Os PS totais foram obtidos por digestões consecutivas com papaína em tampão acetato de sódio 0,1 M (pH 5,0, contendo cisteína 5 mM e EDTA 5 mM, seguidas por cromatografia de troca iônica em coluna de DEAE-celulose. As frações obtidas foram concentradas por liofilização e submetidas à eletroforese em gel de agarose a 0,5%. Os ensaios anticoagulantes foram realizados pelo tempo de tromboplastina parcial ativada (TTPA, usando-se plasma de coelho e uma curva padrão de HEP (100 UI mg-1. As extrações (53,96% mostraram diferenças marcantes durante o fracionamento e no grau de resolução dos PS. A espécie apresentou PS com atividade anticoagulante superior a HEP. O TTPA das frações modificou-se acentuadamente entre as extrações, expressando-se de maneira dose-dependente e sofrendo um acréscimo de 110,40 (1a extração para 143,10 UI mg-1 (3a extração. Os resultados sugerem que a atividadeanticoagulante dos PS isolados de Halymenia sp. foi promovida pela inibição da via intrínseca e/ou comum da cascata de coagulação. As modificações no TTPA possivelmente serão elucidadas pelos mecanismos de ação envolvidos na coagulação e caracterização estrutural desses compostos. Portanto, a rodofícea Halymenia sp. é uma boa fonte de heparinoides e sugerem-se estudos relacionados ao cultivo da espécie, em proteção aos bancos de algas.The increasing demand for heparin (HEP has led to a search for alternative sources of natural anticoagulants. This study aimed to evaluate the anticoagulant activity of sulfated polysaccharides (SP isolated from a Halymenia rhodophyceae genus native to the coast of Ceará, Brazil. Total SP were obtained by consecutive

  17. O peso ao nascer influencia o estado nutricional ao final do primeiro ano de vida? Does birth weight affect nutritional status at the end of first year of life?

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    Maria Eugênia Farias Almeida Motta

    2005-10-01

    Full Text Available OBJETIVO: Analisar a associação entre o baixo peso ao nascer e o estado nutricional ao final do primeiro ano de vida. MÉTODOS: Foi realizado estudo caso-controle aninhado a uma coorte em quatro municípios da Zona da Mata Meridional de Pernambuco. Os recém-nascidos foram recrutados para a coorte nas primeiras 24 horas de vida, sendo o peso aferido ao nascimento e ao final do primeiro ano de vida. Durante o primeiro ano de vida, foram realizadas duas visitas domiciliares semanais para confirmar aleitamento materno predominante e ocorrência de episódios diarréicos. Considerou-se caso (risco nutricional a criança com índice peso/idade abaixo do percentil 10 (n = 117; e controle aquela com percentil igual ou maior que 10 (n = 411. Realizou-se análise de regressão logística hierarquizada para detectar os fatores determinantes do estado nutricional no primeiro ano de vida. RESULTADOS: Os fatores que explicaram o risco nutricional ao final do primeiro ano de vida foram peso ao nascer e ausência de sanitário no domicílio. As crianças que nasceram com peso entre 1.500 g e 2.499 g tiveram uma chance 29 vezes maior (IC 95% = 9,77-87,49 de apresentar risco nutricional aos 12 meses de idade em relação àquelas com peso de nascimento maior que 3.500 g. Nas que residiam em domicílio sem sanitário, a chance foi três vezes maior (IC 95% = 1,54-6,22 em relação àquelas com sanitário com descarga no domicílio. CONCLUSÃO: O baixo peso ao nascer é um dos principais fatores responsáveis pelo risco nutricional ao final do primeiro ano de vida, sendo imprescindível adotar estratégias para sua redução e prevenção.OBJECTIVE: To evaluate the association between low birth weight and nutritional status at the end of the first year of life. METHODS: This was a nested case-control study within a cohort. The study was carried out at maternity hospitals in four cities in the Zona da Mata Meridional in Pernambuco state, Brazil. Newborn infants

  18. Apoptosis of human tongue squamous cell carcinoma cell (CAL-27 induced by Lactobacillus sp. A-2 metabolites

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    Guoliang ZHANG

    2014-07-01

    Full Text Available Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells. Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2 were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL and assayed by methyl thiazolyltetrazolium (MTT method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao fluorescent staining; flow cytometry method (FCM and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2. Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent. Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

  19. Ao leitor sem medo

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    José Eisenberg

    2000-05-01

    Full Text Available O texto resenha Ao leitor sem medo, de Renato Janine Ribeiro (Belo Horizonte, UFMG, 1999.This text is a review of Ao leitor sem medo by Renato Janine Ribeiro (Belo Horizonte, UFMG, 1999

  20. Larvas de Geometridae (Lepidoptera e seus parasitóides, associadas a Erythroxylum microphyllum St.- Hilaire (Erythroxylaceae Geometrid larvae (Lepidoptera and their parasitoids, associated to Erythroxylum microphyllum St.-Hilaire (Erythroxylaceae

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    Gláucia Marconato

    2008-01-01

    Full Text Available O trabalho foi realizado em área de cerrado, em São Carlos, São Paulo, Brasil. Foram realizadas amostragens quinzenais de junho/1999 a junho/2000, por meio de guarda-chuva-entomológico. Foram registradas 22 espécies de Geometridae pertencentes a 14 gêneros; quanto aos parasitóides, 11 gêneros de Hymenoptera e dois de Diptera, Tachinidae. A ocorrência de Cyclomia mopsaria (Geometridae foi de 83%. Outros Geometridae registrados foram: Glena unipennaria, G. bipennaria, G. demissaria, G. brachia, Physocleora junctilinea, P. cariaria, Physocleora sp., Iridopsis fulvitincta, I. nigraria, Hymenomima amberia, Macaria regulata, Ischnopteris inornata., Prochoerodes onustaria, Prochoerodes sp., Thyrinteina arnobia, Nematocampa sp., Melanolophia sp., Isochromodes sp., Semaeopus lunifera, S. vizaria. Os Hymenoptera foram: Microcharops peronata, Charops sp., Metopius sp., Hyposoter sp., Mesochorus sp.(Ichneumonidae; Aleiodes sp., Meteorus sp., Glyptapanteles sp., Protapanteles sp. (Braconidae; Euplectrus sp. (Eulophidae. Os Diptera, Tachinidae foram: Winthemia sp.(Winthemiini e uma espécie de Blondeliini. O período de maior ocorrência de larvas de Geometridae e baixa taxa de parasitismo foi ao final da estação chuvosa e início da estação seca.This work was carried out in a cerrado area, in São Carlos, São Paulo, Brazil. The samples were collected at each two weeks, from June/1999 to June/2000, with entomological umbrella. 22 species of Geometridae arranged in 14 genera were found; concerning parasitoids, 11 genera of Hymenoptera and two genera of Diptera, Tachinidae. The occurrence of Cyclomia mopsaria (Geometridae was 83%. Other Geometridae recorded were: Glena unipennaria, G. bipennaria, G. demissaria, G. brachia, Physocleora junctilinea, P. cariaria, Physocleora sp., Iridopsis fulvitincta, I. nigraria, Hymenomima amberia, Macaria regulata, Ischnopteris inornata, Prochoerodes onustaria, Prochoerodes sp., Thyrinteina arnobia, Nematocampa

  1. Levantamento de estoques da ostra Crassostrea sp. em bancos naturais no litoral paranaense

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    Euler Batista Erse

    2011-09-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2008v21n2p57 A importância econômica de diversas espécies de ostras nativas, em especial a Crassostrea sp., torna atrativa a exploração desta espécie pelas comunidades litorâneas. A explotação deste recurso natural, baseada somente no extrativismo não sustentável acaba por ameaçar os estoques naturais com a sobrepesca. Baseado neste fato, uma avaliação das condições dos estoques naturais desse animal torna-se importante para futuros projetos de conservação e manejo sustentáveis do recurso. Neste trabalho foram levantados dados referentes aos estoques da ostra Crassostrea sp. de três bancos naturais distintos, sendo estes, um na Ilha da Cotinga, na foz do Rio Maciel e outro na foz do Rio Biguaçú. Em cada banco, foram feitas três amostragens aleatórias durante a maré baixa sizígia utilizando-se de um quadrado de amostragem com dimensão de 30x30 cm. Observamos também neste trabalho que, dos locais de estoques naturais da ostra nativa Cras- sostrea sp. presentes na Baía de Paranaguá, o da Ilha da Cotinga apresenta signifi cativamente os maiores valores quanto ao tamanho se comparados com a o Rio Maciel e o Rio Biguaçú, sendo que, entre os dois últimos, o primeiro apresentou maiores valores. Porém, não houve diferenças signifi cativas nos valores biométricos encontrados entre os bancos amostrados nos respectivos bosques.

  2. NCBI nr-aa BLAST: CBRC-TNIG-22-0185 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0185 ref|NP_103831.1| putative manganese transport protein MntH [Mesor...hizobium loti MAFF303099] sp|Q98I99|MNTH_RHILO Probable manganese transport protein mntH dbj|BAB49617.1| manga

  3. Contribuições da Arte ao Atendimento Educacional Especializado e à Inclusão Escolar

    Directory of Open Access Journals (Sweden)

    Libéria Rodrigues NEVES

    Full Text Available RESUMO este texto objetiva propor uma discussão acerca das contribuições da Arte ao Atendimento Educacional Especializado, direcionado ao público-alvo da Educação Especial na perspectiva da Educação Inclusiva. Com base na teoria da Cognição Imaginativa, busca-se apresentar a arte como área de conhecimento dotada de características que dialogam com os objetivos dos trabalhos desenvolvidos nas Salas de Recursos Multifuncionais, oferecidos aos alunos com deficiências, transtorno global do desenvolvimento e altas habilidades e três modelos de trabalho com Arte e Inclusão são relatados: 1 uma oficina desenvolvida na ONG Nosso Sonho (SP, reunindo seis crianças com paralisia cerebral, descrita em Tese de doutorado em Artes Visuais (Escola de Comunicações e Artes da Universidade de São Paulo; 2 um livro que sistematiza quinze anos de trabalho do Instituto Rodrigo Mendes (RJ, que desenvolve projetos em arte-educação para sujeitos com e sem deficiência; e, 3 o trabalho da ONG Corpo Cidadão, que, em uma de suas unidades de educação social, desenvolve oficinas de arte com alunos de escola especial juntamente com alunos de escolas regulares, na grande Belo Horizonte. Enfocando a criação e imaginação como uma atividade humana, e não como uma faculdade dada a priori, o ensaio propõe que a arte deve ser um componente nas atividades desenvolvidas nos espaços de Atendimento Educacional Especializado, direcionadas, principalmente, aos alunos com deficiências intelectuais.

  4. *sp131*I-3-iodobenzylguanidine (*sp131*I-3-IBG) as a scintigraphic agent for the visualization of adrenal medulla tumors

    International Nuclear Information System (INIS)

    Heggeli, D.E.; Brorson, B.I.; Bremper, P.O.

    1983-06-01

    A method of labelling 3-iodobenzylguanidine with *sp131*I is described. 3-IBG . 0.5 H*sb2*SO*sb4* and Cu(II)SO*sb4* were dissolved in a 0.1 M NH*sb4*H*sb2*PO*sb4* buffer and mixed with *sp131*I-NaI. The solution was evaporated to dryness by heating. After addition of water, the solution was heated with reflux for two hours. The I*sp-* ions were removed after labelling by anionic exchange chromatography. The final product was made isotonic and bacteriostatic by the addition of acetate buffer, saline and benzylalcohol. The product was filtered through a membrane filter with a pore size of 0.22*my*m and was apyrogenically tested by limulus test. The tumors of adrenal medulla, pheochromocytomas and neuroplastomas may in some cases be small or located extra-adrenally. In those cases *sp131*I-labelled 3-IBG is a valuable tool, since 3-IBG concentrates in adrenal medulla tumors because ot its analogy to the catecholamines. Injecting a dose of 0.5 mCi *sp131*I-3-IBG (2.5 mCi/mg), which is an adult dose, allows the scintigraphic localization of the tumours, thus guiding the surgeon. Adrenal uptake in mice and dog is described in the report, as well as a rapid method for the control of radiochemical purity. The radioactive concentration of the *sp131*I-3-IBG has been found to be important for the radiochemical stability of the product. (RF)

  5. Bioremediation of crude oil waste contaminated soil using petrophilic consortium and Azotobacter sp.

    Directory of Open Access Journals (Sweden)

    M. Fauzi

    2016-01-01

    Full Text Available This study was aimed to determine the effect Petrophilic and Azotobacter sp. consortium on the rate of degradation of hydrocarbons, Azotobacter growth, and Petrophilic fungi growth in an Inceptisol contaminated with crude oil waste originating from Balongan refinery, one of Pertamina (Indonesia’s largest state-owned oil and gas company units in Indramayu – West Java. This study was conducted from March to April 2014 in the glasshouse of research station of the Faculty of Agriculture, Padjadjaran University at Ciparanje, Jatinangor District, Sumedang Regency of West Java. This study used a factorial completely randomized design with two treatments. The first treatment factor was Petrophilic microbes (A consisting of four levels (without treatment, 2% Petrophilic fungi, 2% Petrophilic bacteria, and the 2% Petrophilic consortium, and Azotobacter sp. The second treatment factor was Azotobacter sp. (B consisting of four levels (without treatment, 0.5%, Azotobacter sp., 1% Azotobacter sp., and 1.5% Azotobacter sp. The results demonstrated interaction between Petrophilic microbes and Azotobacter sp. towards hydrocarbon degradation rate, but no interaction was found towards the growth rate of Azotobacter sp. and Petrophilic fungi. Treatments of a1b3 (2% consortium of Petrophilic fungi with 1.5% Azotobacter sp. and a3b3 (2% Petrophilic consortium and 1.5% Azotobacter sp. had hydrocarbon degradation rate at 0.22 ppm/day for each treatment, showing the highest hydrocarbon degradation rate.

  6. Xanthobacter sp. C20 contains a novel bioconversion pathway for limonene

    OpenAIRE

    Werf, M.J. van der; Keijzer, P.M.; Schaft, P.H. van der

    2000-01-01

    Xanthobacter sp. C20 was isolated from sediment of the river Rhine using cyclohexane as sole source of carbon and energy. Xanthobacter sp. C20 converted both enantiomers of limonene quantitatively into limonene-8,9-epoxide, a not previously described bioconversion product of limonene. With (4R)-limonene, (4R,8R)-limonene-8,9-epoxide was formed as the only reaction product, while (4S)-limonene was converted into a (78:22) mixture of (4S,8R)- and (4S,8S)-limonene-8,9-epoxide. Cytochrome P-450 w...

  7. Two new chroman derivations from the endophytic Penicillium sp. DCS523.

    Science.gov (United States)

    Li, Jun-Tian; Fu, Xiao-Li; Tan, Chun; Zeng, Ying; Wang, Qi; Zhao, Pei-Ji

    2011-01-18

    Strain DCS523 was isolated from the branch tissue of Daphniphyllum longeracemosum and determined to be a Penicillium sp. according to the ITS sequence analysis. The extracts from the PDA solid fermentation media of Penicillium sp. DCS523 were purified to give two new chroman derivatives as well as six known compounds. Based on their spectral data the new compounds were identified as (Z)-6-acetyl- 3-(1,2-dihydroxypropylidene)-5-hydroxy-8-methylchroman-2-one and 6-acetyl-2α,5- dihydroxy-2-(2-hydroxypropyl)- 3α,8-dimethylchroman, respectively.

  8. Identification of Serratia marcescens SE1 and determination of its Herbicide 2,2-dichloropropionate (2,2-DCP Degradation Potential

    Directory of Open Access Journals (Sweden)

    Abel, E.

    2012-01-01

    Full Text Available Aims: The goal of the study is to isolate species of bacteria that capable of utilizing 2,2-dichloropropionic acid (2,2-DCP as sole carbon source from soil sample collected from surrounding lake water located in Universiti Teknologi Malaysia, Skudai, Johor. Methodology and Results: Genomic DNA from bacterium SE1 was extracted and PCR amplification was carried out using universal primers, Fd1 (5’ - AGA GTT TGA TCC TGGCTC AG - 3’ and rP1 (5’- ACG GTC ATA CCT TGT TAC GAC TT - 3’ before sending for sequencing. The 16S rDNA nucleotide sequences were compared with Basic Local Alignment Search Tool nucleotide (BLASTn and further analyzed using phylogenetic tree of Neighbour-Joining method (MEGA 5. Phylogenetic analysis indicated that SE1 strain clearly shared 97% homology to the genus of Serratia marcescens and therefore designated as Serratia marcescens sp. SE1. SE1 exhibited the ability to utilize 2,2-DCP as sole carbon source at 20 mM concentration with cell doubling time of 5 h and maximum chloride ion release of 38 μmolCl-/mL. This result suggests that the dehalogenase enzyme present in the bacteria has high affinity towards the substrate. Based on morphological and partial biochemical characteristics, strain SE1 was a non-motile Gram negative bacterium with red colonies, that gave a catalase positive reaction. Conclusion, significance and impact of study: A better understanding of dehalogenases enzyme produce by this S. marcescens sp. SE1 in general will be useful to be used as bioremediation tools for environmental management. This is the first reported case that Serratia sp. has the ability to degrade halogenated compound.

  9. NCBI nr-aa BLAST: CBRC-TNIG-22-0185 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0185 ref|NP_642362.1| putative manganese transport protein MntH [Xanth...omonas axonopodis pv. citri str. 306] sp|Q8PKX1|MNTH_XANAC Probable manganese transport protein mntH gb|AAM36898.1| manga

  10. Characterization of cycP gene expression in Achromobacter xylosoxidans NCIMB 11015 and high-level heterologous synthesis of cytochrome c' in Escherichia coli.

    Science.gov (United States)

    Harris, Roger L; Barbieri, Sonia; Paraskevopoulos, Kostas; Murphy, Loretta M; Eady, Robert R; Hasnain, S Samar; Sawers, R Gary

    2010-01-01

    The cycP gene encoding a periplasmic cytochrome c' from the denitrifying beta-proteobacterium Achromobacter xylosoxidans was characterized. The genes flanking cycP encode components of a mobile genetic element characteristic of the beta-proteobacteria, suggesting that cycP has inserted within a transposon or insertion element. The gene therefore does not form part of a denitrification operon or gene cluster. The level of expression of the cycP gene and the level of synthesis of its corresponding gene product were found to increase by maximally 3-fold anaerobically. Expression of cycP appears to occur mainly by non-specific read-through transcription from portions of the insertion element. Conditions were developed for high-level overproduction of cytochrome c' in Escherichia coli, which resulted in signal peptide cleavage concomitant with secretion of the protein into the periplasm. Using a single-step purification, 20-30 mg of pure protein were isolated from a 1-litre culture. Based on UV-visible spectrophotometry the dimeric protein was shown to have a full complement of haem and to be indistinguishable from the native protein purified from A. xylosoxidans. This system provides an excellent platform to facilitate biochemical and structural dissection of the mechanism underlying the novel specificity of NO binding to the proximal face of the haem.

  11. Detection of Cryptosporidium sp. in non diarrheal faeces from children, in a day care center in the city of São Paulo, Brazil Detecção de Cryptosporidium sp. em fezes não diarréicas de crianças, em uma escola de educação infantil de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Therezinha T. Carvalho-Almeida

    2006-02-01

    Full Text Available The protozoan Cryptosporidium sp. has been frequently detected in faeces from children with persistent diarrhoea. This work achieved to investigate an outbreak of cryptosporidiosis, in a day care center, attending children of high socio-economic level, between 0 and six years old. The outbreak was detected through the network of public health, when stool samples, not diarrhoeic, were examined at the Parasitology Service of the Adolfo Lutz Institute. Among the 64 examined children, 13 (20.3% showed oocysts of Cryptosporidium sp. in the faeces examined by Kinyoun technique: seven children one year old, three, two years old and three, three years old. Among the 23 examined adults, only a 22 years old woman, possibly having an immunocomprometiment, was positive. Clinical and epidemiological aspects were investigated by questionnaires, highlighting the occurrence of the outbreak in a very dry period.O protozoário Cryptosporidium sp. tem sido detectado com freqüência em fezes de crianças com diarréia persistente. Este trabalho teve como objetivo investigar um surto de criptosporidiose, em uma escola de educação infantil, que atende crianças de classe média alta, de 0 a seis anos de idade. O surto foi detectado a partir de amostras fecais não diarréicas encaminhadas ao Instituto Adolfo Lutz através da rede de saúde pública. Das 64 crianças 13 (20,3% apresentaram oocistos de Cryptosporidium nas fezes, examinadas pelo método de Kinyoun, sendo sete crianças com um ano de idade, três com dois anos de idade e três, com três anos de idade. Dos 23 adultos examinados, apenas uma mulher de 22 anos, com possível imunocomprometimento, foi positiva. Aspectos epidemiológicos e clínicos foram investigados por meio de questionários, ressaltando-se que o surto ocorreu em período muito seco.

  12. INSETICIDAS APLICADOS VIA TRATAMENTO DE SEMENTES VISANDO AO CONTROLE DAS MOSCAS BRANCAS (Bemisia tabaci, GENN. E MINADORA (Liriomyza sp. NA CULTURA DO FEIJOEIRO CHEMICAL CONTROL OF THE WHITE FLY Bemisia tabaci GENN. AND LEAFMINER Liriomyza sp. IN BEAN CROPS BY SEED TREATMENTS

    Directory of Open Access Journals (Sweden)

    Antônio Lopes da Silva

    2007-09-01

    Full Text Available

    Realizou-se o presente experimento no campo experimental da Escola de Agronomia da Universidade Federal de Goiás, de março a maio de 1993, para avaliar o controle das moscas brancas (Bemisia tabaci, GENN. e minadora (Liriomyza sp. na fase inicial da cultura do feijoeiro, com produtos aplicados via tratamento de sementes. Os tratamentos constaram de: imidacloprid 700 PM nas dosagens de 140, 210, 280 e 350 gramas de ingrediente ativo (i.a/l00 kg de sementes; carbosulfan + zinco 250 TS nas dosagens de 375 e 500g i.a./100kg de sementes, comparados com o carbofuran 350 TS na dosagem de 525 g i.a./100kg de sementes (padrão. Pelos resultados, concluiu-se que todos os tratamentos foram eficientes no controle da mosca minadora, com porcentagens de eficiência que variaram de 93 a 99%. Imidacloprid, a partir de 280g i.a./100kg de sementes, foi igual aos outros produtos em eficiência no controle da mosca branca, com porcentagens de controle variando entre 83 a 89%.

    A trial to control the white fly Bemisia tabaci, GENN. and leafminer Liriomyza sp. was carried out in Goiânia, state of Goiás. The treatments and dosages of the insecticides per 100kg of seed were: imidacloprid (140, 210, 280 and 350g a.i., carbofuran (525 g a.i., carbosulfan (375 and 500g a.i, plus an untreated check. The application of the treatments were made on the seeds. The results of the experiment showed that all insecticides were efficient in controlling the leafminer at all dosages tested and imidacloprid at the dosages of 280 and 350g a.i. per 100kg seed was similar in controlling the white fly in bean crops.

  13. Do Fundef ao Fundeb: avaliando o passado para pensar o futuro: um estudo de caso no município de Pirapozinho-SP Del Fundef al Fundeb: evaluación del pasado para pensar el futuro: un estudio de caso en el municipio de Pirapozinho - SP From Fundef to Fundeb: evaluating the past to think about the future: a case study in the municipality of Pirapozinho - SP

    Directory of Open Access Journals (Sweden)

    Regina Celia Ramos

    2011-09-01

    Full Text Available Este artigo, que envolve a combinação de procedimentos quantitativos e qualitativos, traz resultados de pesquisa que analisou o impacto do Fundef na estruturação da rede municipal de ensino de Pirapozinho-SP, e mostra como estes resultados trazem subsídios para um funcionamento mais adequado da atual política de financiamento da educação. Constatou-se que, mesmo sem aumentar os recursos financeiros do ensino fundamental, o Fundef racionalizou e tornou mais eficiente a sua aplicação, diminuindo desvios e desperdícios, e que a instituição dos Conselhos de Acompanhamento e Controle Social possibilitou maior participação popular na fiscalização do executivo aumentando a transparência dos gastos com educação, que a constituição do Fundef abriu a possibilidade de implantação do Fundeb atualmente em vigor e que a melhoria no funcionamento deste está relacionada ao aumento da participação da sociedade nas decisões da política educacional e ao investimento na qualificação desta participação.Este artículo, que combina procedimientos cuantitativos y cualitativos, ofrece resultados de encuesta que analizó el impacto del FUNDEF en la estructuración de la red municipal de Pirapozinho-SP, Brasil, y muestra como estos resultados proporcionan subsidios para un funcionamiento más adecuado de la política actual de financiación de la educación. Se verificó que, aun sin aumentar los recursos financieros de la enseñanza fundamental, el FUNDEF hizo una aplicación más eficiente, y redujo el mal uso. También que el establecimiento de los Consejos de Supervisión y Control Social proporcionan una mayor participación popular en la supervisión del poder ejecutivo y aumentan la transparencia del gasto en educación, que la constitución del FUNDEF abrió la posibilidad de implantación del FUNDEB actualmente en vigor y la mejora de su funcionamiento está relacionado con una mayor participación de la sociedad en las decisiones

  14. Zhongshania antarctica gen. nov., sp. nov. and Zhongshania guokunii sp. nov., gammaproteobacteria respectively isolated from coastal attached (fast) ice and surface seawater of the Antarctic.

    Science.gov (United States)

    Li, Hui-Juan; Zhang, Xi-Ying; Chen, Chun-Xiao; Zhang, Yan-Jiao; Gao, Zhao-Ming; Yu, Yong; Chen, Xiu-Lan; Chen, Bo; Zhang, Yu-Zhong

    2011-09-01

    Two Gram-negative, motile, aerobic, catalase- and oxidase-positive, rod-shaped strains, designated ZS5-23(T) and ZS6-22(T), were respectively isolated from Antarctic coastal attached (fast) ice and surface seawater samples. Both strains could grow at 4-35 °C (optimum 30 °C) and in the absence of NaCl. Analyses of 16S rRNA gene sequences revealed that strains ZS5-23(T) and ZS6-22(T) were closely related to each other (99.0 % sequence similarity) and belonged to the class Gammaproteobacteria, with their closest relatives being Spongiibacter and Melitea species (93.1-94.3 % sequence similarity). The predominant cellular fatty acids in both strains were C₁₇:₁ω8c, C₁₇:₀ and summed feature 3 (C₁₆:₁ω7c and/or iso-C₁₅:₀ 2-OH). Genomic DNA G+C contents of strains ZS5-23(T) and ZS6-22(T) were 51.5 and 51.8 mol%, respectively. The DNA-DNA relatedness between strains ZS5-23(T) and ZS6-22(T) was 50.9 %. Strains ZS5-23(T) and ZS6-22(T) could be differentiated from each other and from Spongiibacter and Melitea species by differences in a number of phenotypic properties. Based on the data presented, strains ZS5-23(T) and ZS6-22(T) represent two novel species in a new genus in the class Gammaproteobacteria, for which the names Zhongshania antarctica gen. nov., sp. nov. (the type species) and Zhongshania guokunii sp. nov. are proposed. The type strain of Zhongshania antarctica is ZS5-23(T) ( = KACC 14066(T)  = CCTCC AB 209246(T)) and that of Zhongshania guokunii is ZS6-22(T) ( = KACC 14532(T)  = CCTCC AB 209247(T)).

  15. SRAO: the first southern robotic AO system

    Science.gov (United States)

    Law, Nicholas M.; Ziegler, Carl; Tokovinin, Andrei

    2016-08-01

    We present plans for SRAO, the first Southern Robotic AO system. SRAO will use AO-assisted speckle imaging and Robo-AO-heritage high efficiency observing to confirm and characterize thousands of planet candidates produced by major new transit surveys like TESS, and is the first AO system to be capable of building a comprehensive several-thousand-target multiplicity survey at sub-AU scales across the main sequence. We will also describe results from Robo-AO, the first robotic LGS-AO system. Robo-AO has observed tens of thousands of Northern targets, often using a similar speckle or Lucky-Imaging assisted mode. SRAO will be a moderate-order natural-guide-star adaptive optics system which uses an innovative photoncounting wavefront sensor and EMCCD speckle-imaging camera to guide on faint stars with the 4.1m SOAR telescope. The system will produce diffraction-limited imaging in the NIR on targets as faint as mν = 16. In AO-assisted speckle imaging mode the system will attain the 30-mas visible diffraction limit on targets at least as faint as mν = 17. The system will be the first Southern hemisphere robotic adaptive optics system, with overheads an order of magnitude smaller than comparable systems. Using Robo-AO's proven robotic AO software, SRAO will be capable of observing overheads on sub-minute scales, allowing the observation of at least 200 targets per night. SRAO will attain three times the angular resolution of the Palomar Robo-AO system in the visible.

  16. Atividade anti-helmíntica de plantas nativas do continente americano: uma revisão Anthelmintic activity of native American plants: a review

    Directory of Open Access Journals (Sweden)

    R.G. Sousa

    2013-01-01

    Full Text Available O presente trabalho faz uma revisão de plantas nativas do continente americano com atividade anti-helmíntica com a finalidade de subsidiar pesquisas e o desenvolvimento de novos medicamentos na área de produtos naturais. Na literatura, foram citadas 22 famílias de plantas, tais como: Moraceae, Asteraceae e Cucurbitaceae, e estudadas cerca de 35 espécies, como a Ficus sp, Cucurbita máxima, e Ruta graveolens, usadas por sua alegada atividade anti-helmíntica. Destas espécies, 26 foram ativas contra alguns helmintos: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus, e Hymenolepsis diminuta. Foi verificado maior número de estudos com parasitas de animais, possivelmente devido ao desenvolvimento de resistência dos helmintos aos tratamentos convencionais disponíveis.This paper is a review of native American plants showing anthelmintic activity in order to give support to research and development of new drugs in the field of natural products. In the literature, 22 plant families were cited, such as: Moraceae, Asteraceae and Cucurbitaceae, and around 35 species were studied, including Ficus sp, Cucurbita maxima and Ruta graveolens, used for their putative anthelmintic activity. From these species, 26 were active against some helminthes: Strongyloides stercolaris, Ancylostomidae, Trichostrongylus sp, Capillaria sp, Ascaris lumbricoides, Taenia saginata, Haemonchus contortus and Hymenolepsis diminuta. Data indicated a larger number of studies with animal parasites, probably due to the resistance of these helminthes to the conventional treatments available.

  17. Atividade enzimática, produção de slime e sensibilidade a antifúngicos de Candida sp Enzymatic activity, slime production and antifungal agent sensitivity of Candida sp

    Directory of Open Access Journals (Sweden)

    Jaqueline Otero Silva

    2007-06-01

    Full Text Available A habilidade de Candida spp secretar enzimas extracelulares e slime tem sido associada como fatores de patogenicidade. Do total de 37 cepas de Candida sp, 100% foram produtoras de proteinase, 83,8% fosfolipase, 64,9% slime e 100% sensíveis ao fluconazol e itraconazol. Foram encontradas 17 tipagens (enzima/slime. Esta metodologia apresentou um bom índice discriminatório (D=0,93 podendo ser utilizado na caracterização fenotípica das leveduras.Abilith of Candida spp to secrete extracellular enzymes and slime has been associated as pathogenicity factors. Out of a total of 37 strains of Candida sp, 100% were proteinase producers, 83.8% were phospholipase producers, 64.9% were slime producers and 100% were sensitive to fluconazole and itraconazole. Seventeen typings (enzymes/slime were found. This methodology presented a good discrimination rate (D = 0.93 and could be used for phenotypic characterization of yeasts.

  18. Use of Bacillus pumilus CBMAI 0008 and Paenibacillus sp. CBMAI 868 for colour removal from paper mill effluent Emprego de Bacillus pumilus CBMAI 0008 e Paenibacillus sp. CBMAI 868 para remoção da cor do efluente da indústria papeleira

    Directory of Open Access Journals (Sweden)

    Patrícia Lopes de Oliveira

    2009-06-01

    Full Text Available Bacillus pumilus and Paenibacillus sp. were applied on the paper mill effluent to investigate the colour remotion. Inocula were individually applied in effluent at pH 7.0, 9.0 and 11.0. The real colour and COD remotion after 48h at pH 9.0 were, respectively, 41.87% and 22.08% for B. pumilus treatment and 42.30% and 22.89% for Paenibacillus sp. Gel permeation chromatography was used to verify the molar masses of compounds in the non-treated and treated effluent, showing a decrease in the compounds responsible for the paper mill effluent colour.Bacillus pumilus e Paenibacillus sp. foram aplicados separadamente no efluente da indústria papeleira a pH 7,0, 9,0 e 11,0, para verificação da remoção da cor e da DQO. As remoções da cor real e DQO após 48h a pH 9,0 foram, respectivamente, de 41,87% e 22,08% após o tratamento com B. pumilus e 42,30% e 22,89% após tratamento com Paenibacillus sp. As massas molares dos compostos presentes no efluente não tratado e tratado foram determinadas por cromatografia de permeação em gel. O emprego dos microrganismos reduziu os compostos responsáveis pela cor do efluente da indústria papeleira.

  19. Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers

    Directory of Open Access Journals (Sweden)

    Liping Xu

    2014-01-01

    Full Text Available A total of 100 Sporisorium scitamineum isolates were investigated by inter simple sequence repeat (ISSR and single primer-sequence related amplified polymorphism (SP-SRAP markers. These isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (PCA of the ISSR, SP-SRAP, or ISSR + SP-SRAP data set. The total gene diversity (Ht and gene diversity between subpopulations (Hs were estimated to be 0.34 to 0.38 and 0.22 to 0.29, respectively, by analyzing separately the ISSR and SP-SRAP data sets, and to be 0.26–0.36 by analyzing ISSR + SP-SRAP data set. The gene diversity attributable to differentiation among populations (Gst was estimated to be 0.35 and 0.22, and the gene flow (Nm was 0.94 and 1.78, respectively, when analyzing separately ISSR and SP-SRAP data set, and was 0.27 and 1.33, respectively, when analyzing ISSR + SP-SRAP data set. Our study showed that there is considerable genetic variation in the analyzed 100 isolates, and the environmental heterogeneity has played an important role for this observed high degree of variation. The genetic differentiation of sugarcane smut fungus depends to a large extent on the heterogeneity of their habitats and is the result of long-term adaptations of pathogens to their ecological environments.

  20. Propaganda de álcool e associação ao consumo de cerveja por adolescentes

    Directory of Open Access Journals (Sweden)

    Roberta Faria

    2011-06-01

    Full Text Available OBJETIVO: Analisar a associação entre propaganda de álcool e o consumo de cerveja por adolescentes. MÉTODOS: Foram entrevistados 1.115 estudantes de 7º e 8º anos de três escolas públicas de São Bernardo do Campo, SP, em 2006. As variáveis independentes foram: atenção prestada às propagandas de álcool, crença na veracidade das propagandas, resposta afetiva às propagandas, uso prévio de cigarro, entre outras. A variável dependente foi consumo de cerveja nos últimos 30 dias. Análises de regressões logísticas univariada e múltipla foram realizadas. Idade, importância dada à religião e ter banheiro em casa foram utilizadas como controle. RESULTADOS: O consumo de cerveja nos últimos 30 dias esteve associado ao uso de cigarro (OR = 4,551, ter uma marca preferida de bebida alcoólica (OR = 5,150, não ser monitorado pelos pais (OR = 2,139, achar que as festas que freqüentam parecem-se com as de comerciais (OR = 1,712, prestar muita atenção aos comerciais (OR = 1,563 e acreditar que os comerciais falam a verdade (OR = 2,122. Essa associação manteve-se mesmo na presença de outras variáveis associadas ao seu consumo. CONCLUSÕES: As propagandas de bebidas alcoólicas associam-se positivamente ao consumo recente de cerveja, por remetem os adolescentes à própria realidade ou por fazê-los acreditar em sua veracidade. Limitar a veiculação de propagandas de bebidas alcoólicas pode ser um dos caminhos para a prevenção do uso e abuso de álcool por adolescentes.

  1. Two New Chroman Derivations from the Endophytic Penicillium sp. DCS523

    Directory of Open Access Journals (Sweden)

    Qi Wang

    2011-01-01

    Full Text Available Strain DCS523 was isolated from the branch tissue of Daphniphyllum longeracemosum and determined to be a Penicillium sp. according to the ITS sequence analysis. The extracts from the PDA solid fermentation media of Penicillium sp. DCS523 were purified to give two new chroman derivatives as well as six known compounds. Based on their spectral data the new compounds were identified as (Z-6-acetyl- 3-(1,2-dihydroxypropylidene-5-hydroxy-8-methylchroman-2-one (1 and 6-acetyl-2α,5- dihydroxy-2-(2-hydroxypropyl- 3α,8-dimethylchroman (2, respectively.

  2. DA PSICODINÂMICA DO TRABALHO AO DIREITO FUNDAMENTAL AO CONTEÚDO DO PRÓPRIO TRABALHO E AO MEIO AMBIENTE ORGANIZACIONAL SAUDÁVEL

    Directory of Open Access Journals (Sweden)

    Leonardo Vieira Wandelli

    2015-10-01

    Full Text Available O presente artigo procura traçar alguns elementos que pretendem articular como, a partir do discurso jurídico, podem ser significados os aportes sobre saúde e trabalho, especialmente aqueles trazidos pela abordagem da Psicodinâmica do Trabalho. Um caminho, assim, que vai dos estudos sobre sujeito, trabalho e saúde até o Direito Constitucional, que afirma como seu fundamento a dignidade humana, indissociável da centralidade antropológica do trabalho; um vínculo expressado pelo direito fundamental ao trabalho que, aqui, se propõe reconstruir a partir do desvelamento de um direito fundamental ao conteúdo do próprio trabalho, articulado ao conceito de meio ambiente organizacional do trabalho. 

  3. Silencing the SpMPK1, SpMPK2, and SpMPK3 Genes in Tomato Reduces Abscisic Acid—Mediated Drought Tolerance

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    Yan Liang

    2013-11-01

    Full Text Available Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA-induced and hydrogen peroxide (H2O2-induced stomatal closure and enhanced ABA-induced H2O2 production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H2O2 levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants’ drought tolerance by influencing stomatal activity and H2O2 production via the ABA-H2O2 pathway.

  4. DESTINAÇÃO FINAL DE RESÍDUOS SÓLIDOS: ENFOQUE GEOTÉCNICO AO ATERRO SANITÁRIO DE PRESIDENTE VENCESLAU/SP

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    José Guilherme Marques Chitero

    2015-09-01

    Full Text Available This paper presents a discussion regarding the geotechnical conditions that must be considered in a solid waste landfill, using as a case study the landfill Wenceslas President - SP, pointing out the conditions investigated in the project area. Therefore, the research was developed through surveys in the field and technical advice on the municipal landfill Wenceslas President - SP. Observe and identify the geotechnical characteristics of the installed project and possible impacts to the conditions of natural resources such as groundwater and soil in the area where the landfill is installed. Trying to analyze studies and data that matches the structure of the site, its influence within the watershed in which it is as well as the local geology. The results indicated poor management of the enterprise, actual contamination of groundwater and geotechnical instability risks,since the operation of the landfill is compromised due to the characteristics presented.

  5. agradecimento aos consultores ad hoc

    Directory of Open Access Journals (Sweden)

    REA Editor

    2012-02-01

    Full Text Available Agradecemos aos professores Nildo Viana (UFG e Flavio Sofiati (UFG, organizadores do DOSSIÊ JUVENTUDE & SOCIEDADE, e aos Consultores Ad hoc pela leitura e apreciação crítica dos artigos submetidos e publicados nesta edição.

  6. Combate ao niilismo e ao totalitarismo em Camus

    Directory of Open Access Journals (Sweden)

    Emanuel Ricardo Germano

    2013-05-01

    Full Text Available A partir de uma leitura da peça Calígula, elaborada entre 1941 e 1943, procurar-se-á contribuir na restituição das preocupações éticas, políticas e filosóficas em cena no teatro de Albert Camus, analisando o sentido de sua expressão teatral no contexto de dois combates,ao niilismo filosófico e ao totalitarismo político de seu tempo. Observaremos em Calígula um importante registro do amadurecimento das leituras de duas heranças filosóficas relevantes na formação intelectual de Camus, Nietzsche e Heráclito, à luz da experiência do totalitarismo, observando e ressaltando com isso a amplitude de seu intertexto filosófico e a importância do aporte ético e político de Camus como pensador multifacetado, engajado nos enfrentamentos históricos e solicitações críticas de seu tempo.

  7. A Prediction of the Damping Properties of Hindered Phenol AO-60/polyacrylate Rubber (AO-60/ACM) Composites through Molecular Dynamics Simulation

    Science.gov (United States)

    Yang, Da-Wei; Zhao, Xiu-Ying; Zhang, Geng; Li, Qiang-Guo; Wu, Si-Zhu

    2016-05-01

    Molecule dynamics (MD) simulation, a molecular-level method, was applied to predict the damping properties of AO-60/polyacrylate rubber (AO-60/ACM) composites before experimental measures were performed. MD simulation results revealed that two types of hydrogen bond, namely, type A (AO-60) -OH•••O=C- (ACM), type B (AO-60) - OH•••O=C- (AO-60) were formed. Then, the AO-60/ACM composites were fabricated and tested to verify the accuracy of the MD simulation through dynamic mechanical thermal analysis (DMTA). DMTA results showed that the introduction of AO-60 could remarkably improve the damping properties of the composites, including the increase of glass transition temperature (Tg) alongside with the loss factor (tan δ), also indicating the AO-60/ACM(98/100) had the best damping performance amongst the composites which verified by the experimental.

  8. Adsorption of *sp134*Cs onto two different types of roof materials

    International Nuclear Information System (INIS)

    Quenild, C.; Tveten, U.

    1983-01-01

    The report relates to a subproject under a Nordic project called ''Large reactor accidents - consequences and mitigating actions''. Natural decontamination of two types of roof material, typical of Norwegian and Swedish buildings, was examined. The roofs were contaminated with *sp134*Cs. After two days with 7 mm precipitation, the remaining activity on the tar-paper roof was 97% and on the steel roof 29%. After 8 months the remaining activity was 61% on the tar-paper roof and 22% on the steel roof. In spring *sp134*Cs was spread upon an 8 cm thick layer of fresh snow on an additional tar-paper roof. After 3 montsh and 191 mm precipitation the remaining activity was 59%. All results are based upon *sp134*Cs in collected run-off water.(Auth.)

  9. Metabolites of the endophytic fungus Penicillium sp. FJ-1 of Acanthus ilicifolius.

    Science.gov (United States)

    Liu, Jian-Fang; Chen, Wei-Jie; Xin, Ben-Ru; Lu, Jie

    2014-06-01

    Two new compounds, named as (2R,3S)-pinobanksin-3-cinnamate (1), and 15alpha-hydroxy-(22E,24R)-ergosta-3,5,8(14),22-tetraen-7-one (2), were isolated from the endophytic fungus Penicillium sp. FJ-1 of Acanthus ilicifolius Linn. Their structures were elucidated on the basis of spectroscopic analysis. Additionally, compound 1 exhibited potent neuroprotective effects on corticosterone-damaged PC12 cells, and compound 2 showed potent cytotoxicity on glioma cell lines.

  10. Crescimento e sobrevivência de duas procedências de Aspidosperma polyneuron em plantios experimentais em Bauru, SP. Growth and survival of two provenances of Aspidosperma polyneuron Müll. in experimental plantation, in Bauru, SP.

    Directory of Open Access Journals (Sweden)

    Aida Sanae SATO

    2008-06-01

    Full Text Available A exploração desordenada das matasnativas vem causando o esgotamento davariabilidade genética de muitas espécies de valoreconômico e ambiental. Desde a década de 1960,o Instituto Florestal de São Paulo vem desenvolvendoestudos pioneiros em conservação ex situ dealgumas espécies nativas ameaçadas e de interesseeconômico, como, por exemplo, Aspidospermapolyneuron, visando à conservação, ao melhoramentogenético e ao conhecimento do seu comportamentosilvicultural. Em 1964 foi implantado um experimentocom duas procedências de A. polyneuron: Bauru(SP e Alvorada do Sul (PR, em uma Unidade deConservação do Instituto Florestal, em Bauru/SP.Foi utilizado o delineamento de blocos ao acaso,com nove plantas por parcela, espaçamento 3 x 3 me seis repetições. Em 2006, foram avaliados odiâmetro à altura do peito (DAP, cm, altura total(HT, m, forma do fuste (F, volume cilíndricoindividual (VCi, m3, volume cilíndrico por hectare(VCha, m3 ha-1 e sobrevivência (S, %. Os resultadosdemonstraram que a procedência Bauru apresentoumaior produção volumétrica de madeira por hectare(VCha e que os valores das demais variáveis nãodiferiram estatisticamente entre as duas procedências.As informações obtidas neste estudo sãoimportantes para fornecer subsídios para avaliar aviabilidade de plantios comerciais e experimentaisde A. polyneuron visando à conservação e aomelhoramento genético desta espécie.The disorderly exploitation of nativeforests has caused the depletion of the geneticvariability of many species of economic andenvironmental value. Since the 1960 decade,the Forestry Institute of São Paulo has beendeveloping pioneering studies of ex situconservation of some endangered native treespecies and with economic interests, as for example,Aspidosperma polyneuron aiming the conservation,the tree breeding and the knowledge of thesilvicultural behavior. In 1964 an experiment withtwo provenances was implanted: A. polyneuron,Bauru (SP

  11. Caracterización de tilapia roja (Oreochromis sp. con marcadores moleculares RAPD Characterization of the red tilapia Oreochromis sp. through molecular markers RAPD

    Directory of Open Access Journals (Sweden)

    Julieta Torres Jaramillo

    2010-04-01

    Full Text Available Se utilizó la técnica RAPD (amplificación al azar de ADN polimórfico para el estudio de la diversidad genética de Oreochromis sp. (tilapia roja en cinco piscícolas del Valle del Cauca (Colombia y en la determinación del nivel de introgresión de las especies parentales Oreochromis mosambicus, O. niloticus y O. aureus. Se evaluaron 25 cebadores, ocho fueron polimórficos y se obtuvieron 109 bandas. Los valores de heterocigosidad esperada (0.196 a 0.256 y la estructura genética (Gst = 0.22 para Oreochromis sp. indicaron un elevado grado de polimorfismo y alta estructuración genética. Estos resultados fueron consistente con el Fst = 0.268 (P Random amplified polymorphic DNA (RAPD markers were used to study genetic diversity on red Tilapia (Oreochromis sp. species collected from five fish farms located in the Valle del Cauca, Colombia and to determine the level of introgression from three parental species O. mosambicus, O. niloticus and O. aureus into local Oreochromis populations. from the 25 RAPD primers evaluated, eight were polymorphic and 109 banding patterns were observed, any of them were specific. The expected levels of heterozygosis (0.1964 to 0.2561 and genetic structure (Gst = 0.22 funded for Oreochrosmis sp. indicate high grade of polymorphism and genetic structuring. This results were observed following the analysis of molecular variance [AMOVA] (Fst = 0.268 (P <0.0001 and Multiple correspondence analysis (Gst = 0.040. The values of genetic similarity, the analysis of group, the analysis of multiple correspondence and the level of introgression, indicated that the differences in the introgression levels(P=0.0001 were significant. The low level of observed genetic differentiation among populations, could be the result of fish with the same genetic origin, whereas the high variation within populations can be displayed by handling practices and the pressure of selection to favor commercial phenotypes. The level of introgression

  12. Antibacterial Actions and Potential Phototoxic Effects of Volatile oils of Foeniculum sp. (fennel, Salvia sp. (sage, Vitis sp. (grape, Lavandula sp. (lavender

    Directory of Open Access Journals (Sweden)

    Elif Ayse Erdogan Eliuz

    2016-09-01

    Full Text Available In the present study, the volatile compounds of essential oil of Foeniculum vulgare (fennel, Salvia officinalis (sage, Vitis vinifera (grape, Lavandula angustifolia (lavender were analysed by gas chromatography-mass spectrometry (GC-MS using the Nist and Willey libraries. It was determined that the main components of Foeniculum sp. were anethole (41.11%, carvacrol (9.18%. whereas main components of Salvia sp were 1.8 cineole (34.09%, caryophyllene (10.95%, camphor (9.44%, α-pinene (8.42%. Vitis sp. contained linoleic acid (36.98%, 2,4-decadienal (30.79%. Finally, volatile component of Lavandula sp. was linalool (33.57%, linalyl acetate (30.74%. Photoxic antibacterial activity of volatile oil of those plants against Escherichia coli (ATCC 25293, Klebsiella pneumoniae (10031, Salmonella thyphimurium, Bacillus subtilis (ATCC 6633, Staphylococcus aureus (ATCC 25925, Enterococcus feacalis (ATCC 29212 were examined by using disc diffusion method. We demonstrated that volatile oil effectively can be activated by a standard LED light. In vitro, significant phototoxicity was demonstrated by volatile oil of Foeniculum sp. and Vitis sp. (P < 0.05, while minor phototoxicity was induced by Lavandula sp. Therefore, volatile oil of plant can be considered as a potential photosensitizer in the photochemical therapy.

  13. Bioactive Indole Derivatives from the South Pacific Marine Sponges Rhopaloeides odorabile and Hyrtios sp.

    Directory of Open Access Journals (Sweden)

    Marie-Lise Bourguet-Kondracki

    2011-05-01

    Full Text Available Indole derivatives including bromoindoles have been isolated from the South Pacific marine sponges Rhopaloeides odorabile and Hyrtios sp. Their structures were established through analysis of mass spectra and 1D and 2D NMR spectroscopic data. Their potential inhibitory phospholipase A2 (PLA2, antioxidant and cytotoxic activities were evaluated. The new derivative 5,6-dibromo-l-hypaphorine (9 isolated from Hyrtios sp. revealed a weak bee venom PLA2 inhibition (IC50 0.2 mM and a significant antioxidant activity with an Oxygen Radical Absorbance Capacity (ORAC value of 0.22. The sesquiterpene aureol (4, also isolated from Hyrtios sp., showed the most potent antioxidant activity with an ORAC value of 0.29.

  14. NCBI nr-aa BLAST: CBRC-TNIG-22-0328 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0328 sp|P43141|ADB4C_MELGA Beta-4C adrenergic receptor (Beta-4C adreno...ceptor) (Beta-4C adrenoreceptor) gb|AAA62151.1| beta-4C-adrenergic receptor gb|AAA62150.1| adrenergic beta-4c receptor P43141 3e-31 63% ...

  15. Descriptions of Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina) from Haryana, India.

    Science.gov (United States)

    Tomar, V V S; Somvanshi, Vishal S; Bajaj, Harish K

    2015-03-01

    Two different nematodes were isolated from the bark of Albizia lebbeck trees; one from insect infested and another from noninfested, healthy tree. Based on the biological, morphological, and molecular evidences, the nematodes are described as Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina). Deladenus albizicus n. sp., isolated from insect-infested tree, multiplied on the fungus Nigrospora oryzae. Myceliophagous females of this nematode reproduced by parthenogenesis and spermathecae were indistinct. Infective females, readily produced in the cultures, are dorsally curved. Only one type of males containing small-sized sperms in their genital tracts were produced in the culture. Myceliophagous females: L = 0.75 to 1.71 mm, a = 32.3 to 50.8, b = 9.3 to 11.2, b' = 5.2 to 7.3, c = 27.2 to 35.6, V = 91.0 to 93.3, c' = 2.0 to 2.9, stylet = 11 to 12 µm, excretory pore in the region of median pharyngeal bulb, 43 to 47 µm anterior to hemizonid. Deladenus processus n. sp., isolated from bark of healthy A. lebbeck tree, was cultured on Alternaria alternata. Myceliophagous females reproduced by amphimixis and their spermathecae contained rounded sperms. Infective females were never produced, even in old cultures. Myceliophagous females: L = 0.76 to 0.99 mm, a = 34 to 49, b = 13.3 to 17.7, b' = 3.8 to 5.8, c = 19.6 to 22.8, V = 92.2 to 93.5, c' = 2.7 to 3.5, stylet = 6 to 7 µm, excretory pore in the proximity of hemizonid, tail conoid, tapering from both sides to a long pointed central process. It is proposed to classify Deladenus species in three groups: durus, siricidicola, and laricis groups based on female and spermatogonia dimorphism, mode of reproduction, and insect parasitism.

  16. Bradysia sp. em morangueiro Bradysia sp. in strawberry

    Directory of Open Access Journals (Sweden)

    Bernadete Radin

    2009-04-01

    Full Text Available No trabalho, relatam-se os primeiros registros de Bradysia sp. (Insecta: Diptera: Sciaridae em morangueiro (Fragaria x ananassa Duch., cultivado no Município de Eldorado do Sul, RS. O cultivo foi realizado em sacolas com três metros de comprimento, preenchidas com substrato composto de casca de arroz e turfa, dispostas horizontalmente sobre bancadas de madeira, em ambiente protegido. A presença de Bradysia sp. foi observada na segunda quinzena de agosto de 2005. Neste trabalho, estão descritos os sintomas apresentados no morangueiro pela praga, prováveis conseqüências sobre o aparecimento de doenças e uma breve descrição morfológica da Bradysia sp., adulto e fase larval.This paper describes the first record of Bradysia sp. (Insecta; Diptera; Sciaridae in strawberry (Fragaria x ananassa, cultivated in the city of Eldorado do Sul, RS, Brazil. Strawberry was planted in plastic bags filled with a mixture of burnt rice hulls and peat and cultivated in a greenhouse. The presence of Bradysia sp was noticed in the second fortnight of August, 2005. The symptoms in strawberry and the probable consequences in terms of disease arising were described in the present study, as well as the morphological characterization of Bradysia sp. and its illustrations.

  17. Origem botânica de cargas de pólen de colmeias de abelhas africanizadas em Piracicaba, SP Botanic origin of pollen beehives’ loads from Africanized honeybees in Piracicaba, SP, Brazil

    Directory of Open Access Journals (Sweden)

    Anna Frida Hatsue Modro

    2011-11-01

    Full Text Available Este estudo objetivou identificar a origem botânica das cargas de pólen coletadas por abelhas africanizadas. Em Piracicaba (SP, foram instaladas, durante as quatro estações do ano, cinco colmeias de A. mellifera com um coletor de pólen frontal em cada uma. A preparação palinológica foi pelo uso de acetólise, sendo identificados e contados aproximadamente 900 grãos de pólen por amostra. A partir da composição polínica, calculou-se a riqueza, os índices de diversidade e equitabilidade. Ao longo do ano, foram encontrados 81 tipos polínicos, pertencentes a 32 famílias botânicas sendo Fabaceae, Asteraceae e Malvaceae, as famílias com maior frequência de tipos polínicos (≥5 tipos polínicos, e Myrtaceae, a família com dois tipos polínicos (Eucalyptus sp. e Myrcia sp. entre os nove mais frequentes nas amostras (>10%. A maior riqueza de tipos polínicos foi no verão e a maior diversidade e equitabilidade, na primavera. Fabaceae, Asteraceae, Malvaceae e Myrtaceae são as famílias botânicas mais importantes como fontes poliníferas em Piracicaba - SP.This study aimed to identify the botanic origin of pollen loads collected by Africanized honeybees. In Piracicaba (SP, during four seasons of the year, five honeybee colonies were installed with a frontal pollen collector in each one. The palinological preparation was done by the use of acetolysis and identified and counted approximately 900 pollen grains per sample. From the pollinic composition, the richness, diversity indexes and equitability were calculated. Throughout the year, 81 pollinic types were found, belonging to 32 botanic families, being Fabaceae, Asteraceae and Malvaceae the ones with the biggest frequency of pollinic types (≥5 pollinic types and, Myrtaceae, the family with two pollinic types (Eucalyptus sp. and Myrcia sp., among the nine most common types in the samples (> 10%. The biggest richness of pollinic types was during summer, and the biggest diversity and

  18. Análise quantitativa das células de Langerhans em mucosa bucal de pacientes submetidos ao transplante de medula óssea alogênico com doença enxerto contra hospedeiro crônica

    OpenAIRE

    Érika Sinara Lenharo Orti-Raduan

    2007-01-01

    A doença enxerto contra hospedeiro é uma complicação comum nos pacientes submetidos ao transplante de medula óssea alogênico. Com o objetivo de contribuir para o esclarecimento da participação das células de Langerhans na doença enxerto contra hospedeiro crônica (GVHDc) quando de sua ocorrência na mucosa bucal, foram analisados 40 pacientes oncohematológicos e hematológicos submetidos ao transplante de medula óssea alogênico no Hospital Amaral Carvalho, Jaú - SP. Cortes microscópicos de 3µm d...

  19. Bioaugmentation with endophytic bacterium E6S homologous to Achromobacter piechaudii enhances metal rhizoaccumulation in host Sedum plumbizincicola

    Directory of Open Access Journals (Sweden)

    Ying eMa

    2016-02-01

    Full Text Available Application of hyperaccumulator–endophyte symbiotic systems is a potential approach to improve phytoremediation efficiency, since some beneficial endophytic bacteria are able to detoxify heavy metals, alter metal solubility in soil and facilitate plant growth. The objective of this study was to isolate multi-metal resistant and plant beneficial endophytic bacteria and to evaluate their role in enhancing plant growth and metal accumulation/translocation. The metal resistant endophytic bacterial strain E6S was isolated from stems of the Zn/Cd hyperaccumulator plant Sedum plumbizincicola growing in metalliferous mine soils using Dworkin and Foster salts minimal agar medium with 1-aminocyclopropane-1-carboxylate (ACC as the sole nitrogen source, and identified as homologous to Achromobacter piechaudii based on morphological and biochemical characteristics, partial 16S rDNA sequence and phylogenetic analysis. Strain E6S showed high level of resistance to various metals (Cd, Zn and Pb. Besides utilizing ACC, strain E6S exhibited plant beneficial traits, such as solubilization of phosphate and production of indole-3-acetic acid. Inoculation with E6S significantly increased the bioavailability of Cd, Zn and Pb in soil. In addition, bacterial cells bound considerable amounts of metal ions in the following order: Zn ˃ Cd ˃ Pb. Inoculation of E6S significantly stimulated plant biomass, uptake and bioaccumulation of Cd, Zn and Pb. However, E6S greatly reduced the root to shoot translocation of Cd and Zn, indicating that bacterial inoculation assisted the host plant to uptake and store heavy metals in its root system. Inoculation with the endophytic bacterium E6S homologous to A. piechaudii can improve phytostabilization of metalliferous soils due to its effective ability to enhance in situ metal rhizoaccumulation in plants.

  20. Occurrence of /sup 22/Na in some species of Canadian biota

    Energy Technology Data Exchange (ETDEWEB)

    Guthrie, J E

    1965-09-01

    Cosmic-ray spallation of argon and fallout from testing of high-yield nuclear weapons have increased the amount of /sup 22/Na present in the environment. Consequently, it has been possible to measure /sup 22/Na in Alaskan natives, caribou, elk, and some foods. Samples of flora and fauna were routinely collected in the vicinity of Pinawa, Manitoba, as a part of the Whiteshell Nuclear Research Establishment's radiation survey program. Gamma spectrometric analysis methods revealed the presence of /sup 22/Na in samples taken during 1964, in addition to other long-lived fission products such as /sup 144/Ce, /sup 125/Sb, /sup 106/Ru, /sup 137/Cs, and /sup 54/Mn. Sodium-22 was measured in samples of muscle tissue taken from the red squirrel (Tamiasciurus sp.) and hare (Lepus americanus), but was not detected in the flesh of the short-tailed weasel (Mustela erminea). Sodium-22 was detected neither in the sweet (white) clover which grew profusely in the area where the mammals were trapped nor in composite samples of Winnipeg River water in any of the species of fish taken from the river during this period: rock bass (Amblopletes rupestris), sucker (Castostomus sp.), pike (Esox lucius), pickerel (Stizostedion vitreum), and sauger (Stizostedion canadense). However, /sup 22/Na has been reported in bass caught in the Columbia River, Washington. Potassium-40 and /sup 137/Cs means in the samples examined indicate an apparent increase in the amounts of /sup 137/Cs. The amount of /sup 22/Na measured in rabbits is much less than the maximum permissible body burden for the nuclide (10 ..mu..C).

  1. Jovens de Santo André, SP, Brasil: Um estudo sobre valores em diferentes grupos sociais

    OpenAIRE

    Sheila Aparecida Ferreira Lachtim

    2010-01-01

    Esta dissertação se propõe a: apreender valores sociais contemporâneos, notadamente aqueles referidos ao consumo de drogas, discutir diferenças entre esses valores nos diferentes grupos sociais; e analisar possíveis relações entre esses valores. Trata-se de um estudo qualitativo de caráter exploratório, descritivo e analítico que utilizou um roteiro de entrevista semi-estruturada para apreensão do objeto. Um total de 86 jovens participou, de diferentes regiões do município de Santo André, SP,...

  2. Efeito de extratos vegetais no controle de Fusarium oxysporum f. sp tracheiphilum em sementes de caupi Effect of naturals extracts on the control of Fusarium oxysporum f. sp tracheiphilum in cowpea seeds

    Directory of Open Access Journals (Sweden)

    Jandiê Araújo da Silva

    2009-04-01

    Full Text Available A fusariose é uma doença de grande importância para a cultura do feijoeiro em virtude da redução da produtividade. O controle da doença vem sendo realizado com aplicações indiscriminadas de fungicidas químicos, acarretando problemas ao homem e ao meio ambiente. Objetivou-se, no presente trabalho, avaliar o efeito de extratos vegetais no controle de Fusarium oxysporum f. sp tracheiphilum, proveniente de sementes de caupi, comparando-se com o efeito do fungicida químico. Utilizaram-se extratos de alho, angico e manjericão, isolados ou combinados entre si e ou em associação ao fungicida Mancozeb. Uma alíquota de 50 µL de cada tratamento foi adicionada em orifício feito no centro das placas de Petri com BDA, sobre o qual depositou-se um disco de micélio do fungo. Avaliou-se durante sete dias, medindo-se o crescimento micelial do patógeno. Sementes de caupi foram desinfestadas com hipoclorito de sódio a 4,0%, semeadas em sacos de polietileno contendo solo autoclavado. Oito dias após germinação, efetuaram-se ferimentos no colo das plântulas, aplicando-se suspensão de esporos do fungo (1,4 x 10-5 con/mL. As avaliações de severidade da doença foram realizadas diariamente durante 30 dias após a inoculação, utilizando-se escala de notas. Os extratos combinados de alho + manjericão e angico + manjericão não inibiram o crescimento micelial do fungo, enquanto o extrato de manjericão isoladamente, proporcionou o menor crescimento micelial, indicando a ação fungicida e inibitória desse tratamento sobre o fungo F. oxysporum f. sp tracheiphilum nas condições analisadas. O fungicida Mancozeb + o extrato de angico proporcionou menor média de severidade nas plantas avaliadas.Wilt caused by Fusarium sp. in cowpea crop is a disease of great importance because it causes yield decrease. The control of this disease has been accomplished with uncontrolled applications of chemical fungicides causing problems for humans and

  3. INTERAKSI ANTARA Trichoderma Harzianum, Penicillium SP. DAN Pseudomonas SP. SERTA KAPASITAS ANTAGONISMENYA TERHADAP Phytophthora CapsicilN VITRO*[Interaction Among Trichoderma Harzianum, Penicillium SP., Pseudomonas SP. and Antagonism Capacities Against Phy

    OpenAIRE

    Suharna, Nandang

    2003-01-01

    A preliminary study has been done to know antagonism capacities of three isolates of Trichoderma harzianum, two isolates of Penicillium sp.and one isolate of Pseudomonas sp.against Phytophthora capsici in vitro and interaction among those six antagonists.The highest antagonism capacity possessed by Penicillium sp. KN1, respectively followed by Penicillium sp.KN2,Pseudomonas sp. GH1 and the three T. harzianum isolates. Except for those three T. harzianum isolates, the two Penicillium sp.isolat...

  4. Ocorrência de Taenia sp. na população atendida no laboratório central do Instituto Adolfo Lutz, São Paulo, SP, Brasil (1960/1989 Occurrence of Taenia sp. in the population attended in the central laboratory of "Instituto Adolfo Lutz", São Paulo, SP, Brazil (1960/1989

    Directory of Open Access Journals (Sweden)

    Rosa Maria Donini Souza Dias

    1991-04-01

    Full Text Available Foram examinados retrospectivamente os relatórios mensais e anuais da Seção de Enteroparasitoses do Laboratório Central do Instituto Adolfo Lutz, São Paulo, SP, do período de 1960 a 1989, perfazendo uma série histórica de 30 anos, com 1.519.730 exames protoparasitológicos e 355 identificações de proglotes de Taenia. Pelo método da sedimentação espontânea foram diagnosticados 7.663 (0,5% casos de presença de ovos de Taenia sp. nas fezes. Das 355 proglotes enviadas para identificação, 311 (87,60% estavam em condições de serem especificadas, e dessas, 273 (87,80% eram proglotes de Taenia saginata e 38 (12,22% de T. solium.Monthly and yearly reports of the Seção de Enteroparasitoses of the Instituto Adolfo Lutz (São Paulo, SP, Brazil from 1960 to 1989 with 1,519,730 parasitological stool examinations were studied. There were also 355 identifications of Taenia sp. proglottids. Using HOFFMAN, PONS & JANER's method, 7,663 (0.5% cases of taeniasis were diagnosed, and 311 (87.60% of the 355 proglottids were on easy terms to be specified, 273 (87.80% of them were from Taenia saginata.

  5. Resposta de diferentes populações de Digitaria insularis ao herbicida glyphosate Response of different Digitaria insularis populations to glyphosate

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    N.M Correia

    2010-12-01

    Full Text Available Objetivou-se com estse trabalho avaliar o controle químico de diferentes populações de capim-amargoso (Digitaria insularis pelo herbicida glyphosate por meio de curva de dose-resposta, além de propor tratamentos alternativos para as populações mais tolerantes. O delineamento experimental foi o de blocos ao acaso, com quatro repetições, em esquema fatorial 5 x 9. As sementes de capim-amargoso foram coletadas em cinco locais: área de produção de grãos da Fazenda de Ensino, Pesquisa e Produção da UNESP, Jaboticabal (SP; área de produção comercial de grãos, localizada nos municípios de Campo Florido-MG e Rio Verde-GO; pomar de laranja, localizado no município de Matão (SP; e área não agrícola sem histórico da aplicação de glyphosate (Jaboticabal-SP. O glyphosate (0D, 1/4D, 1/2D, D, 2D, 4D e 8D, em que D é a dose recomendada de 1,5 kg ha-1 de equivalente ácido e as suas associações [glyphosate + fluazifop-p-butil (1,5 + 0,25 kg ha-1 e glyphosate (1,5 kg ha-1 com sequencial de diuron + paraquat (0,20 + 0,40 kg ha-1 + 0,2% de surfatante] foram pulverizados em plantas de sete a oito perfilhos e altura média de 20 cm. As populações de capim-amargoso de Campo Florido e Rio Verde foram consideradas suscetíveis; as de Jaboticabal e Matão, tolerantes; e a da área não agrícola, de sensibilidade intermediária. A associação de glyphosate ao fluazifop ou a sua aplicação com sequencial de diuron + paraquat foram eficazes no controle das populações mais tolerantes de capim-amargoso.The objective of this study was to evaluate the chemical control of different sourgrass (Digitaria insularis populations by the herbicide glyphosate through dose-response curves, besides considering alternative treatments to control tolerant populations. A randomized block design was used with four replications, in a factorial scheme (5 x 9. Sourgrass seeds were colleted from five locations: a grain production area located at the educational

  6. Burkholderia sp. KCTC 11096BP modulates pepper growth and resistance against Phytophthora capsici

    International Nuclear Information System (INIS)

    Kang, S.M.; Hamayun, M.; Shinwari, Z.K.

    2016-01-01

    Biological control of crop diseases is desirable for sustainable agriculture as it minimizes chemical inputs in the agricultural system and promotes eco-friendly environment. We analyzed the favorable role of Burkholderia sp. KCTC 11096BP against the pathogen Phytophthora capsici in pepper. We screen thirty rhizobateria for their anti-pathogen activity, and found that Burkholderia sp. KCTC 11096BP exhibits maximum growth inhibition of the pathogen P. capsici. The bacterium inoculation to pepper plants significantly enhanced growth attributes of pepper in infected and control treatments. The total proteins (10.9%), and the amino acids viz. glycine (4.08 ug/g), leucine (3.3 ug/g), and alanine (3.26 ug/g) were preset in considerably higher quantities in Burkholderia sp. applied treatments as compare to control. The systemic acquired resistance (SAR) of the host plant was up-regulated by Burkholderia sp. KCTC, as endogenous salicylic acid (235.5 ng/g) and jasmonic acid (22.8 ng/g) levels were found higher in such treatments. It was concluded that Burkholderia sp. KCTC 11096BP mitigates the adverse effects of P. capsici on pepper crop and can improve crop productivity at the field level. (author)

  7. Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. under varying N/P conditions.

    Science.gov (United States)

    Liu, Junzhuo; Vyverman, Wim

    2015-03-01

    The N/P ratio of wastewater can vary greatly and directly affect algal growth and nutrient removal process. Three benthic filamentous algae species Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. were isolated from a periphyton bioreactor and cultured under laboratory conditions on varying N/P ratios to determine their ability to remove nitrate and phosphorus. The N/P ratio significantly influenced the algal growth and phosphorus uptake process. Appropriate N/P ratios for nitrogen and phosphorus removal were 5-15, 7-10 and 7-20 for Cladophora sp., Klebsormidium sp. and Pseudanabaena sp., respectively. Within these respective ranges, Cladophora sp. had the highest biomass production, while Pseudanabaena sp. had the highest nitrogen and phosphorus contents. This study indicated that Cladophora sp. had a high capacity of removing phosphorus from wastewaters of low N/P ratio, and Pseudanabaena sp. was highly suitable for removing nitrogen from wastewaters with high N/P ratio. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Plâncton, Artemia sp, dieta artificial e suas combinações no desenvolvimento e sobrevivência do quinguio (Carassius auratus durante a larvicultura Plankton, Artemia sp, artificial diet and their combinations on the development and survival of goldfish (Carassius auratus during larviculture phase

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    Eliana Maria Galdioli

    2000-05-01

    Full Text Available O objetivo deste estudo foi verificar a influência do uso de diferentes alimentos sobre o desenvolvimento de larvas de quinguio (Carassius auratus, assim como determinar o melhor manejo alimentar. O delineamento experimental utilizado foi o inteiramente casualizado com cinco tratamentos e quatro repetições. Para análise estatística utilizou-se o teste de Tukey. Utilizaram-se 20 aquários com capacidade para 10 L, sendo estocadas 20 larvas com 5,20 mm de comprimento por aquário. Os tratamentos utilizados foram: somente ração com 56,00% de proteína bruta (R; plâncton (PL; náuplios de Artemia sp (NA, ração + plâncton (R+PL e ração + náuplios de Artemia sp (R+NA, sendo seu fornecimento à vontade duas vezes ao dia. Houve mortalidade de todos os indivíduos submetidos ao tratamento R. O uso de NA e R+NA proporcionou taxas de sobrevivência superiores (p 0,05. Conclui-se que as diferentes fontes de alimento interferem no desempenho das larvas, sendo o manejo alimentar com o fornecimento de ração + náuplios de Artemia sp o mais adequado para a larvicultura do quinguioThe aim of this study was to verify the influence of different diets on the development of goldfish (Carassius auratus larvae and determine the best diet management during its larviculture phase. The experimental design was totally randomized with five treatments and four replicatess, using Tukey test for statistical analysis. Twenty 10L-aquariums were used, where twenty larvae with 5.20 mm length were stocked per aquarium. Quinguio larvae were fed daily ration of different diets: ration alone including 56.00% crude protein (R, plankton (PL, Artemia sp nauplii (AN, ration + plankton (R+PL and ration + Artemia sp nauplii (R+AN, ad libitum twice a day. Treatment R caused the death of all individuals. AN and R+AN diets provided a higher survival rate (p 0.05 was observed between the different treatments. Under the conditions of the present experiment, the results led to

  9. Effect of probiotic microorganisms isolated from Hypostomus plecostomus in Oreochromis sp juveniles

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    Yuri Vela-Gutiérrez

    2017-01-01

    Full Text Available Objective. The aim of this study was to assess the behavior of red tilapia juveniles (Oreochromis sp. in presence of the possible probiotic microorganisms isolated from the gastrointestinal tract of panche fish (Hypostomus plecostomus. Materials and methods. During the phase of assessment of probiotics, 120 red tilapia (Oreochromis sp juveniles were selected, with average weight of 1.28±0.22 g and average length of 2.99±0.27 cm. Three experimental treatments were formulated taking into account the nourishment needs of red tilapia (Oreochromis sp.. The statistical method employed was a completely random design. Results. The three strains of Lactobacillus genus (Lactobacillus plantarum, Lactobacillus pentosus, Lactobacillus rhamnosus isolated from the digestive tract of H. plecostomus, yielded diverse results in the biometrical variables considered, as well as in the SGR, PER, AE, AC. Conclusions. The best rapports regarding the gain in length were for the treatment with native probiotics, and it is deduced that they were easily settled in the intestines of the fish (Oreochromis sp.. It is also determined that there are significant differences in the results or gain in length for the Control Concentrate feed (CC against Concentrate feed plus Native Probiotic (CNP for red tilapia (Oreochromis sp. in the juvenile stag for a 30 day time span.

  10. Associação de plasma sanguíneo ao tratamento de envenenamento botrópico em equino: relato de caso

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    A.C. Camplesi

    Full Text Available RESUMO O presente trabalho tem por objetivo relatar um caso de envenenamento botrópico em um equino, fêmea, seis anos de idade, da raça Quarto de Milha, pesando 460kg, que foi atendido no hospital veterinário da FCAV/Unesp, Campus de Jaboticabal, SP. No exame clínico, observou-se aumento bilateral de narina, com extrema sensibilidade ao toque, presença das marcas da presa da serpente na região rostral de focinho, mucosas róseas com petéquias. No exame de sangue, pôde-se detectar alteração no tempo de coagulação sanguínea (>30 minutos. O animal permaneceu internado, sendo instituída a seguinte terapia: soro antiofídico polivalente, transfusão de plasma sanguíneo equino, fluidoterapia intensa, flunixin meglumine e sulfa associado ao trimetoprim. A associação da transfusão de plasma sanguíneo equino ao tratamento convencional foi de extrema importância para correção da coagulopatia causada pelo acidente ofídico. A égua apresentou melhora clínica e resolução do quadro de envenenamento após cinco dias da internação.

  11. MICOBIOTA ASSOCIADA À MADEIRA SERRADA DE Eucalyptus grandis HILL EX MAIDEN DURANTE A SECAGEM AO AR LIVRE

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    Paulo Fernando Trugilho

    2006-02-01

    Full Text Available O objetivo principal deste trabalho foi o de acompanhar a secagem ao ar livre da madeira serrada de Eucalyptus grandis conduzida na região de Lavras, MG e identificar a micobiota associada à madeira em diversas fases da secagem. As tábuas foram cortadas de oito toras de 3,0 m de comprimento, oriundas de três árvores, com 27 anos de idade, plantadas em área experimental da UFLA. Dos resultados, pôde-se concluir que a secagem da madeira de Eucalyptus grandis ao ar livre, iniciada em janeiro, consumiu 158 dias até atingir umidade próxima a 12,5%. A curva de secagem foi representada por uma equação logarítmica com coeficiente de determinação (R2 igual a 98,3%. Os fungos causadores de bolores superficiais e de manchas tiveram maior ocorrência no início da secagem. As mais altas freqüências de fungos, verificadas no início da secagem, foram observadas para Penicillium spp. e Pestalotiopsis sp. O fungo Lentinus lepideus ocorreu com maior freqüência no final da secagem.

  12. First report of Anisakis sp. in Epinephelus sp. in East Indonesia

    OpenAIRE

    Annytha Ina Rohi Detha; Diana Agustiani Wuri; Julianty Almet; Yuni Riwu; Christin Melky

    2018-01-01

    Objective: The present research was conducted to identify the prevalence of Anisakis sp. as fish-borne zoonoses in Epinephelus sp. in territorial waters of East Nusa Tenggara, Indonesia. Materials and methods: A total of 50 fish (Epinephelus sp.) were collected from Kupang Fish Market in East Nusa Tenggara. Identification of Anisakis sp. was performed based on morphological observations considering shape of ventriculus, boring tooth, and mucron using binocular microscope. Results: Prev...

  13. Substance P enhances tissue factor release from granulocyte-macrophage colony-stimulating factor-dependent macrophages via the p22phox/β-arrestin 2/Rho A signaling pathway.

    Science.gov (United States)

    Yamaguchi, Rui; Yamamoto, Takatoshi; Sakamoto, Arisa; Ishimaru, Yasuji; Narahara, Shinji; Sugiuchi, Hiroyuki; Yamaguchi, Yasuo

    2016-03-01

    Granulocyte-macrophage colony stimulating factor (GM-CSF) induces procoagulant activity of macrophages. Tissue factor (TF) is a membrane-bound glycoprotein and substance P (SP) is a pro-inflammatory neuropeptide involved in the formation of membrane blebs. This study investigated the role of SP in TF release by GM-CSF-dependent macrophages. SP significantly decreased TF levels in whole-cell lysates of GM-CSF-dependent macrophages. TF was detected in the culture supernatant by enzyme-linked immunosorbent assay after stimulation of macrophages by SP. Aprepitant (an SP/neurokinin 1 receptor antagonist) reduced TF release from macrophages stimulated with SP. Pretreatment of macrophages with a radical scavenger(pyrrolidinedithiocarbamate) also limited the decrease of TF in whole-cell lysates after stimulation with SP. A protein kinase C inhibitor (rottlerin) partially blocked this macrophage response to SP, while it was significantly inhibited by a ROCK inhibitor (Y-27632) or a dynamin inhibitor (dinasore). An Akt inhibitor (perifosine) also partially blocked this response. Furthermore, siRNA targeting p22phox, β-arrestin 2, or Rho A, blunted the release of TF from macrophages stimulated with SP. In other experiments, visceral adipocytes derived from cryopreserved preadipocytes were found to produce SP. In conclusion, SP enhances the release of TF from macrophages via the p22phox/β-arrestin 2/Rho A signaling pathway. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. APONTAMENTOS SOBRE O PLANEJAMENTO URBANO RELATIVO AO USO DA ÁGUA EM OURINHOS-SP

    Directory of Open Access Journals (Sweden)

    Franciele Miranda Ferreira Dias

    2017-03-01

    Le travail examine dans le contexte de la Géographie urbaine, l'évolution de l'utilisation de l'eau dans Ourinhos - SP, notamment dans la zone urbaine. Ainsi, il fait référence au plan directeur du Padre Lebret du 1955, qui se réfère à la période initiale d'occupation de l'espace urbain de la municipalité. Il y a aussi un effort sur la compréhension du point historique et géographique de vue sur la façon dont la question de l'eau a été traitée dans cette ville, compte tenu de la genèse de Ourinhos jusqu'à ce que les années 1950 mettent l'accent sur l'importance du Rio Paranapanema présenté lors de la genèse le noyau urbain en raison du fait que le lieu de fourniture de matières premières de l'industrie de la poterie locale, étant le même responsable de l'occupation et de l'étalement urbain de la région sud Ourinhos. Mots clés: Plan Directeur; Ourinhos; Géographie Urbaine.

  15. Biodegradation of 3,5-dimethyl-2,4-dichlorophenol in saline wastewater by newly isolated Penicillium sp. yz11-22N2.

    Science.gov (United States)

    Yan, Zhou; He, Huijun; Yang, Chunping; Zeng, Guangming; Luo, Le; Jiao, Panpan; Li, Huiru; Lu, Li

    2017-07-01

    In this study, the performance of 3,5-dimethyl-2,4-dichlorophenol (DCMX) degradation by a screened strain was investigated. 18S rDNA and the neighbor-joining method were used for identification of the isolated strain. The results of phylogenetic analysis and scanning electron micrographs showed that the most probable identity of the screened strain should be Penicillium sp. Growth characteristics of Penicillium sp. and degradation processes of DCMX were examined. Fourier transform infrared spectroscopy of the inoculated DCMX solution was recorded, which supported the capacity of DCMX degradation by the screened Penicillium sp. Under different salinity conditions, the highest growth rate and removal efficiency for DCMX were obtained at pH6.0. The removal efficiency decreased from 100% to 66% when the DCMX concentration increased from 5 to 60mg/L, respectively. Using a Box-Behnken design, the maximum DCMX removal efficiency was determined to be 98.4%. With acclimation to salinity, higher removal efficiency could be achieved. The results demonstrate that the screened Penicillium sp. has the capability for degradation of DCMX. Copyright © 2017. Published by Elsevier B.V.

  16. Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245.

    Science.gov (United States)

    Sayed, Mahmoud; Dishisha, Tarek; Sayed, Waiel F; Salem, Wesam M; Temerk, Hanan A; Pyo, Sang-Hyun

    2016-03-10

    Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245 as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl)butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5:5:2 was found optimum. The optimal conditions for biotransformation were pH 8, 1v/v/m airflow and 500rpm stirring speed. In batch mode of operation, 70.6% of 5g/l TMP was converted to BHMB in 10 days. For recovery of the product the adsorption pattern of BHMB to the anion exchange resin, Ambersep(®) 900 (OH(-)), was investigated in batch and column experiments giving maximum static and dynamic binding capacities of 135 and 144mg/g resin, respectively. BHMB was separated with 89.7% of recovery yield from the fermentation broth. The approach is applicable for selective oxidation of other highly branched polyols by biotransformation. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. CULTIVO EXPERIMENTAL DEL CLADOCERO Moina sp ALIMENTADO CON Ankistrodesmus sp y Saccharomyces cereviseae

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    Martha Prieto

    2006-05-01

    Full Text Available Saccharomyces cereviseae. Materiales y métodos. Fueron realizados cultivos experimentales de la cepade cladóceros Moina sp en el laboratorio de Alimento Vivo de la Universidad de Córdoba, bajo condicionescontroladas de temperatura (22oC, pH (7.6, intensidad lumínica (2000 lux y aireación. Se emplearondos dietas de alimento como tratamiento (Ankistrodesmus y Ankistrodesmus mas Saccharomyces cereviseaeen concentración de 40 x 105 cel.ml-1 cada uno, para determinar su efecto sobre el desempeño de lapoblación. Diariamente se registró la densidad poblacional y celular. Resultados. Se obtuvieron diferenciassignificativas para el efecto de las dietas sobre el crecimiento poblacional de los cladóceros, los individuosalimentados con Ankistrodesmus sp + Saccharomyces cereviseae alcanzaron una densidad de 12.3a±0.30 org/ml-1 presentando mayor tasa instantánea de crecimiento (K 0.36 ± 0.002, el menor tiempode duplicación (td 1.94 ± 0.012 y el mayor rendimiento (r 1,1 ± 0.07 clad.ml-1.día-1. Conclusión. Loscladóceros por sus características de crecimiento en cultivo, presentan adaptación favorable a lascondiciones de manejo para la producción de biomasas potencialmente útiles como partícula nutritivacon fines acuícolas.

  18. Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter.

    Science.gov (United States)

    Houbraken, Jos; López-Quintero, Carlos A; Frisvad, Jens C; Boekhout, Teun; Theelen, Bart; Franco-Molano, Ana Esperanza; Samson, Robert A

    2011-06-01

    Several species of the genus Penicillium were isolated during a survey of the mycobiota of leaf litter and soil in Colombian Amazon forest. Five species, Penicillium penarojense sp. nov. (type strain CBS 113178(T) = IBT 23262(T)), Penicillium wotroi sp. nov. (type strain CBS 118171(T) = IBT 23253(T)), Penicillium araracuarense sp. nov. (type strain CBS 113149(T) = IBT 23247(T)), Penicillium elleniae sp. nov. (type strain CBS 118135(T) = IBT 23229(T)) and Penicillium vanderhammenii sp. nov. (type strain CBS 126216(T) = IBT 23203(T)) are described here as novel species. Their taxonomic novelty was determined using a polyphasic approach, combining phenotypic, molecular (ITS and partial β-tubulin sequences) and extrolite data. Phylogenetic analyses showed that each novel species formed a unique clade for both loci analysed and that they were most closely related to Penicillium simplicissimum, Penicillium janthinellum, Penicillium daleae and Penicillium brasilianum. An overview of the phylogeny of this taxonomically difficult group is presented, and 33 species are accepted. Each of the five novel species had a unique extrolite profile of known and uncharacterized metabolites and various compounds, such as penicillic acid, andrastin A, pulvilloric acid, paxillin, paspaline and janthitrem, were commonly produced by these phylogenetically related species. The novel species had a high growth rate on agar media, but could be distinguished from each other by several macro- and microscopical characteristics.

  19. Prevalência e fatores associados ao uso de drogas ilícitas em gestantes da coorte BRISA

    OpenAIRE

    Priscila Coimbra Rocha; Maria Teresa Seabra Soares de Britto e Alves; Deysianne Costa das Chagas; Antônio Augusto Moura da Silva; Rosangela Fernandes Lucena Batista; Raimundo Antonio da Silva

    2016-01-01

    Resumo O artigo objetiva analisar a prevalência e fatores associados ao uso de drogas ilícitas na gestação. Estudo transversal, aninhado à coorte pré-natal do estudo de coorte BRISA. Utilizou-se medidas de frequência e modelo de regressão logística múltipla hierarquizada. A prevalência estimada foi de 1,45% para o uso de drogas ilícitas, 22,32% para o uso de bebidas alcóolicas e 4,22% para o de cigarro. A população mostrou-se jovem, de 20-34 anos de idade (81%), com escolaridade de 9-11 anos ...

  20. ETIOLOGY OF THE BOVINE CLINICAL MASTITIS IN GOIÂNIA ETIOLOGIA DA MASTITE CLÍNICA BOVINA NA BACIA LEITEIRA DE GOIÂNIA

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    Paulo César Moreira

    2007-09-01

    Full Text Available

    There is an estimate that the mastitis in dairy herds causes production losses between 5 and 35%, equivalent from 85 to 500 million dollars per year. 231 milk samples from 231 cows on different stages of lactation, with clinic mastitis, from 35 farms of Goiânia, were analyzed in order to map the pathogens implicated in these process and to discover the microorganisms with major prevalence. All the samples had positive growth. The principal agents were Staphylococcus coagulase positive (32.90%, Streptococcus sp. (22.07%, Pseudomonas sp. (12.12%, Enterobacter sp. (10.38%, Corynebacterium sp. (8.65%, Escherichia coli (8.22%, Bacillus sp. (8.22%, Proteus sp. (6.49%, Klebsiella sp. (4.32% and Staphylococcus coagulase negative (3.46%. The Nocardia genus was isolated in 0.86% of the cases.

    KEY-WORDS: Bovine mastitis; etiology; isolated microorganisms.

    Estima-se que a presença de mastite bovina em rebanhos produtores provoque perdas de produção entre 5 e 35%, o que equivale de 85 a 500 milhões de dólares ao ano. Com o objetivo de mapear os patógenos envolvidos nesse processo e evidenciar os microrganismos com maior freqüência foram examinadas amostras de leite de 231 vacas, em diferentes estágios de lactação, que apresentaram sinais de mastite clínica e eram pertencentes a 35 propriedades rurais da bacia leiteira de Goiânia. Todas as amostras tiveram crescimento bacteriano positivo. Os principais agentes isolados foram o Staphylococcus coagulase positiva (32,90%, Streptococcus sp. (22,07%, Pseudomonas sp. (12,12%, Enterobacter sp. (10,38%, Corynebacterium sp. (8,65%, Escherichia coli (8,22%, Bacillus sp. (8,22%,

  1. CARACTERIZAÇÃO MORFOMÉTRICA DA MICROBACIA DO RIO BAURU/SP OBTIDA POR TÉCNICAS DE GEOPROCESSAMENTO

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    Sérgio Campos

    2016-03-01

    Full Text Available Esse trabalho objetivou a aplicação de geoprocessamento na caracterização morfométrica da microbacia do Rio Bauru – Bauru (SP através do Sistema de Informação Geográfica – Selva, visando à preservação, racionalização do seu uso e recuperação ambiental.   A microbacia apresenta uma área de 4.049ha e está localizada entre os paralelos 22o 50' 05" a 22o 54' 26" de latitude S e 48o 22' 29" a 48o 26' 36"  de longitude W Gr.  A base cartográfica utilizada foi a carta planialtimétrica de Botucatu (SP, em escala 1:50000 (IBGE, 1973 na  extração das curvas de nível, da hidrografia e da topografia, em ambiente de Sistema de Informações Geográficas - Idrisi Selva, para determinação dos índices morfométricos. Os resultados mostram que os baixos valores da densidade de drenagem, associados à presença de rochas permeáveis, facilitam a infiltração da água no solo, diminuindo o escoamento superficial e o risco de erosão e da degradação ambiental, bem como o baixo valor do fator de forma amparado pelo índice de circularidade indica que a microbacia tende a ser mais alongada com menor susceptibilidade à ocorrência  de enchentes mais  acentuadas. O parâmetro ambiental coeficiente de rugosidade permitiu classificar a microbacia para vocação com floresta e reflorestamento. Palavras-chave: Geoprocessamento; Morfometria; Hidrografia.   MORPHOMETRIC CHARACTERISTICS OF RIVER BAURU/SP WATERSHED OBTAINED BY GIS TECHNIQUES   ABSTRACT This work aimed at the application of geoprocessing in the morphometric characterization of the watershed of the Rio Bauru - Bauru (SP through the Geographic Information System - Selva, aimed at preservation, rational use and environmental restoration. The watershed covers an area of 4.049ha and is located between parallels 22o 50' 05" to 22o 54' 26" S latitude and 48o 22' 29" to 48o 26' 36" W longitude Gr. The base map used was planialtimetric chart of Botucatu (SP, scale 1: 50000

  2. Controle de plantas daninhas na cultura de soja resistente ao glyphosate

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    Núbia Maria Correia

    2010-01-01

    Full Text Available O objetivo da pesquisa foi avaliar o controle de plantas daninhas em área cultivada com soja resistente ao herbicida glyphosate, sem a utilização de práticas complementares de manejo de plantas daninhas. Foram desenvolvidos experimentos, em condições de campo, nos anos agrícolas 2005/2006 e 2006/2007 em Jaboticabal (SP. Foram avaliadas duas cultivares de soja resistentes ao glyphosate (CD 214 RR e M-SOY 8008 RR, oito tratamentos de herbicidas (glyphosate, em aplicação única, nas doses de 0,48; 0,72; 0,96 e 1,20 kg ha-1 de equivalente ácido, associadas ou não a aplicação sequencial na dose de 0,48 kg ha-1, além de duas testemunhas, uma capinada e outra mantida infestada. As cultivares de soja influenciaram na infestação das espécies de plantas daninhas na área. Sem a aplicação de glyphosate, houve o predomínio de X. strumarium na área, desfavorecendo a ocorrência de outras espécies. Quando utilizado glyphosate, independentemente da dose, a infestação contabilizada aos 35 e 40 dias após a primeira aplicação, no primeiro e segundo ano, respectivamente, foi baixa. O controle de plantas daninhas na cultura da soja transgênica é diretamente influenciado pela dose de glyphosate, havendo controle satisfatório com a aplicação única de 0,96 kg ha-1 ou a sequencial de 0,48 + 0,48 kg ha-1 de glyphosate. Em situação de menor infestação (2006/2007, a aplicação única de 0,48 kg ha-1 de glyphosate é suficiente para o controle das plantas daninhas. As cultivares de soja transgênica CD 214 RR e M-SOY 8008 RR influenciam diferencialmente a dinâmica das espécies de plantas daninhas, sendo o controle químico mais efetivo na situação de cultivo de M-SOY 8008 RR, em que houve menor diversidade e desenvolvimento das plantas daninhas.

  3. Coberturas vacinais por doses recebidas e oportunas com base em um registro informatizado de imunização, Araraquara-SP, Brasil, 2012-2014

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    Márcia de Cantuária Tauil

    Full Text Available Resumo OBJETIVO: descrever as coberturas vacinais por tipo de vacina aos 12 e aos 24 meses de idade. Métodos: estudo de coorte descritivo com crianças nascidas em 2012, residentes em Araraquara-SP, Brasil, registradas no Sistema de Informações sobre Nascidos Vivos (Sinasc; realizou-se relacionamento manual dos dados do Sinasc com um registro informatizado de imunização (RII; avaliou-se a situação vacinal pelas recomendações do estado de São Paulo, por doses recebidas e oportunas. Resultados: 2.740 crianças estavam registradas no Sinasc; dessas, 99,6% constavam no RII; entre as 2.612 (95,3% crianças estudadas, a vacina tríplice viral (sarampo, caxumba e rubéola apresentou as menores coberturas aos 12 meses por doses recebidas (74,8% e aos 24 meses por doses oportunas (53,5% e recebidas (88,0%. Conclusão: as coberturas foram superiores a 90% para a maioria das vacinas; entretanto, observou-se atraso vacinal, o que aponta a necessidade de intensificar ações que visem à vacinação oportuna.

  4. Resistência ao intemperismo artificial de cinco madeiras tropicais e de dois produtos de acabamento.

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    Janine Oliveira e Silva

    2010-08-01

    Full Text Available Os objetivos do trabalho foram avaliar o processo de fotodecomposição da madeira de cinco espécies tropicais e analisar o efeito de proteção de dois produtos de acabamento de madeiras quando submetidos ao intemperismo artificial. Foram empregadas as madeiras de ipê-roxo (Tabebuia impeginosa, itaúba (Mezilaurus itauba, maçaranduba (Manilkara huberi, tatajuba (Bagassa guianensis e tauari (Couratari sp.. Amostras destas madeiras foram submetidas a ciclos de radiação ultravioleta (UV e água até perfazer um total de 2.000 horas de irradiação e 400 horas de lixiviação em água. A mudança da cor natural das madeiras, indicativo do processo de fotodegradação, e o grau de proteção de um verniz poliuretânico transparente e de um "stain" semitransparente foram monitorados por espectrocolorimetria. As espécies mais resistentes foram o ipê-roxo e tauari, seguidas pela maçaranduba, itaúba e tatajuba respectivamente. O verniz forneceu proteção por tempo maior ao ipê-roxo e à maçaranduba, enquanto o "stain" foi mais eficiente para a itaúba e tatajuba. Ambos os produtos tiveram desempenho similar para a madeira de tauari.

  5. Naphthalene degradation by bacterial consortium (DV-AL) developed from Alang-Sosiya ship breaking yard, Gujarat, India.

    Science.gov (United States)

    Patel, Vilas; Jain, Siddharth; Madamwar, Datta

    2012-03-01

    Naphthalene degrading bacterial consortium (DV-AL) was developed by enrichment culture technique from sediment collected from the Alang-Sosiya ship breaking yard, Gujarat, India. The 16S rRNA gene based molecular analyzes revealed that the bacterial consortium (DV-AL) consisted of four strains namely, Achromobacter sp. BAB239, Pseudomonas sp. DV-AL2, Enterobacter sp. BAB240 and Pseudomonas sp. BAB241. Consortium DV-AL was able to degrade 1000 ppm of naphthalene in Bushnell Haas medium (BHM) containing peptone (0.1%) as co-substrate with an initial pH of 8.0 at 37°C under shaking conditions (150 rpm) within 24h. Maximum growth rate and naphthalene degradation rate were found to be 0.0389 h(-1) and 80 mg h(-1), respectively. Consortium DV-AL was able to utilize other aromatic and aliphatic hydrocarbons such as benzene, phenol, carbazole, petroleum oil, diesel fuel, and phenanthrene and 2-methyl naphthalene as sole carbon source. Consortium DV-AL was also efficient to degrade naphthalene in the presence of other pollutants such as petroleum hydrocarbons and heavy metals. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. A 3-factor model for the FACIT-Sp.

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    Canada, Andrea L; Murphy, Patricia E; Fitchett, George; Peterman, Amy H; Schover, Leslie R

    2008-09-01

    The 12-item Functional Assessment of Chronic Illness Therapy-Spiritual Well-being Scale (FACIT-Sp) is a popular measure of the religious/spiritual (R/S) components of quality of life (QoL) in patients with cancer. The original factor analyses of the FACIT-Sp supported two factors: Meaning/Peace and Faith. Because Meaning suggests a cognitive aspect of R/S and Peace an affective component, we hypothesized a 3-factor solution: Meaning, Peace, and Faith. Participants were 240 long-term female survivors of cancer who completed the FACIT-Sp, the SF-12, and the BSI 18. We used confirmatory factor analysis to compare the 2- and 3-factor models of the FACIT-Sp and subsequently assessed associations between the resulting solutions and QoL domains. Survivors averaged 44 years of age and 10 years post-diagnosis. A 3-factor solution of the FACIT-Sp significantly improved the fit of the model to the data over the original 2-factor structure (Delta chi(2)=72.36, df=2, p<0.001). Further adjustments to the 3-factor model resulted in a final solution with even better goodness-of-fit indices (chi(2)=59.11, df=1, p=0.13, CFI=1.00, SMRM=0.05).The original Meaning/Peace factor controlling for Faith was associated with mental (r=0.63, p<0.000) and physical (r=0.22, p<0.01) health on the SF-12, and the original Faith factor controlling for Meaning/Peace was negatively associated with mental health (r=-0.15, p<0.05). The 3-factor model was more informative. Specifically, using partial correlations, the Peace factor was only related to mental health (r=0.53, p<0.001); Meaning was related to both physical (r=0.18, p<0.01) and mental (r=0.17, p<0.01) health; and Faith was negatively associated with mental health (r=-0.17, p<0.05). The results of this study support a 3-factor solution of the FACIT-Sp. The new solution not only represents a psychometric improvement over the original, but also enables a more detailed examination of the contribution of different dimensions of R/S to QoL. (c

  7. WEED CONTROL IN GENETICALLY MODIFIED GLYPHOSATE-TOLERANT SOYBEAN MANEJO DE PLANTAS DANINHAS EM SOJA GENETICAMENTE MODIFICADA TOLERANTE AO GLYPHOSATE

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    Júlio Cezar Durigan

    2011-04-01

    convencionais, exigem, além do controle químico, a adoção de outras estratégias de manejo de plantas daninhas. Objetivou-se, com este trabalho, avaliar o controle químico de plantas daninhas, em soja geneticamente modificada (transgênica tolerante ao herbicida glyphosate associado a coberturas vegetais, na entressafra. O experimento foi instalado em área experimental da FCAV/Unesp, Jaboticabal (SP. O delineamento experimental foi o de blocos ao acaso, em esquema de parcelas subdivididas, com quatro repetições. Nas parcelas, foram avaliadas as coberturas vegetais de Brachiaria brizantha (braquiarão cv. Marandu, Pennisetum americanum (milheto forrageiro cv. BN2 e vegetação espontânea, e, nas subparcelas, os herbicidas glyphosate, chlorimuron - ethyl + lactofen em mistura e fluazifop-p-butyl em aplicação sequencial, além de duas testemunhas sem aplicação. A cobertura com braquiarão contribuiu para o controle químico, exercendo supressão das plantas daninhas. A aplicação única de 720 g e.a. ha-1 de glyphosate, independentemente da cobertura vegetal utilizada na entressafra, foi suficiente para o controle adequado de Acanthospermum hispidum, Alternanthera tenella, Amaranthus sp., Bidens pilosa, Xanthium strumarium, Cenchrus echinatus, Digitaria sp. e Eleusine indica, com resultados similares ao tratamento (chlorimuron-ethyl + lactofen + fluazifop-p-buthyl. Comparados à testemunha capinada, os herbicidas testados não afetaram a altura das plantas, massa seca da parte aérea, massa de 100 grãos e a produtividade de grãos. As plantas de soja crescidas sobre os resíduos vegetais de braquiarão e milheto forrageiro apresentaram maior altura, porém, nenhuma outra característica avaliada na cultura foi influenciada pelas coberturas.

    PALAVRAS-CHAVE: Brachiaria brizantha; Pennisetum americanum; plantio direto

  8. Fatty acid composition of Spirulina sp., Chlorella sp. and Chaetoceros sp. microalgae and introduction as potential new sources to extinct omega 3 and omega 6

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    Homan Gorjzdadeh

    2016-05-01

    Full Text Available Background: This study was carried out to determine the oil fatty acids from two special species of microalgae; Spirulina sp.,Chlorella sp. and also Chaetoceros sp. collected from Bahmanshir River. Materials and Methods: Sampling of microalgae Chaetoceros sp. from Bahmanshir River was under taken using bottle samplers during spring season of 2013. Microalgae Spirulina sp. and Chlorella sp. were supplied from Shrimp Research Institute of Iran in Bushehr Province. Samples then were cultured under controlled laboratory conditions and mass culture for 100 liters was undertaken. Isolation of microalgae species from water of cultured media was carried out using filtration and centrifugation methods. The fatty acid compositions were determined by Gas – FID chromatography. Results: Results showed that regarding Saturated Fatty Acids (SFA obtained from purified culture of Chaetoceros sp., Spirulina sp. and Chlorella sp. the maximum amount of total fatty acids were belonged to palmitic acids (C16:0 with 15.21%, 30.1% and 25.17% of total fatty acids  respectively. Analysis of Mono Unsaturated Fatty Acids (MUFA showed that in the Oleic acid was maximum amount of 34% in Spirulina sp. In addition the amount of MUFA in Chlorella sp. was 16.37% of total fatty acids. On the other hand the amount of palmeotic acid in purified culture of Chaetoceros sp. was 30.33% from total content of fatty acids. Analysis of Poly Unsaturated Fatty Acids (PUFA, Linoleic acid (C18:2 (Omega 6, revealed maximum percentage in Spirulina sp. with 18.8%. Results of Alpha linoleic acid (C18:3 (Omega3 analysis showed maximum amount of 9.66% in Chlorella sp. compared to other microalgae with lower omega 3 contents. Spirulina sp. contained maximum amount of Linoleic acid (C18:2 with 18.8% of total fatty acids. Therefore, Spirulina sp. can be considered as a rich source of omega 6 for the purpose of fatty acid extractions. The presence of PUFA in Chlorella sp. and Spirulina sp. was

  9. Suppression of Plant Immune Responses by the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 Type III Effector Tyrosine Phosphatases HopAO1 and HopAO2

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    María Pilar Castañeda-Ojeda

    2017-05-01

    Full Text Available The effector repertoire of the olive pathogen P. savastanoi pv. savastanoi NCPPB 3335 includes two members of the HopAO effector family, one of the most diverse T3E families of the P. syringae complex. The study described here explores the phylogeny of these dissimilar members, HopAO1 and HopAO2, among the complex and reveals their activities as immune defense suppressors. Although HopAO1 is predominantly encoded by phylogroup 3 strains isolated from woody organs of woody hosts, both HopAO1 and HopAO2 are phylogenetically clustered according to the woody/herbaceous nature of their host of isolation, suggesting host specialization of the HopAO family across the P. syringae complex. HopAO1 and HopAO2 translocate into plant cells and show hrpL-dependent expression, which allows their classification as actively deployed type III effectors. Our data also show that HopAO1 and HopAO2 possess phosphatase activity, a hallmark of the members of this family. Both of them exert an inhibitory effect on early plant defense responses, such as ROS production and callose deposition, and are able to suppress ETI responses induced by the effectorless polymutant of P. syringae pv. tomato DC3000 (DC3000D28E in Nicotiana. Moreover, we demonstrate that a ΔhopAO1 mutant of P. savastanoi NCPBB 3335 exhibits a reduced fitness and virulence in olive plants, which supports the relevance of this effector during the interaction of this strain with its host plants. This work contributes to the field with the first report regarding functional analysis of HopAO homologs encoded by P. syringae or P. savastanoi strains isolated from woody hosts.

  10. Suppression of Plant Immune Responses by the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 Type III Effector Tyrosine Phosphatases HopAO1 and HopAO2

    Science.gov (United States)

    Castañeda-Ojeda, María Pilar; Moreno-Pérez, Alba; Ramos, Cayo; López-Solanilla, Emilia

    2017-01-01

    The effector repertoire of the olive pathogen P. savastanoi pv. savastanoi NCPPB 3335 includes two members of the HopAO effector family, one of the most diverse T3E families of the P. syringae complex. The study described here explores the phylogeny of these dissimilar members, HopAO1 and HopAO2, among the complex and reveals their activities as immune defense suppressors. Although HopAO1 is predominantly encoded by phylogroup 3 strains isolated from woody organs of woody hosts, both HopAO1 and HopAO2 are phylogenetically clustered according to the woody/herbaceous nature of their host of isolation, suggesting host specialization of the HopAO family across the P. syringae complex. HopAO1 and HopAO2 translocate into plant cells and show hrpL-dependent expression, which allows their classification as actively deployed type III effectors. Our data also show that HopAO1 and HopAO2 possess phosphatase activity, a hallmark of the members of this family. Both of them exert an inhibitory effect on early plant defense responses, such as ROS production and callose deposition, and are able to suppress ETI responses induced by the effectorless polymutant of P. syringae pv. tomato DC3000 (DC3000D28E) in Nicotiana. Moreover, we demonstrate that a ΔhopAO1 mutant of P. savastanoi NCPBB 3335 exhibits a reduced fitness and virulence in olive plants, which supports the relevance of this effector during the interaction of this strain with its host plants. This work contributes to the field with the first report regarding functional analysis of HopAO homologs encoded by P. syringae or P. savastanoi strains isolated from woody hosts. PMID:28529516

  11. Karyotypes of Akodon orophilus Osgood 1913 and Thomasomys sp. (Rodentia: Sigmodontinae from Huánuco, Peru

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    Víctor Pacheco

    2012-10-01

    Full Text Available Conventional chromosomal preparations were made of three native mice from Huánuco, Peru: a male and a female of Thomasomys sp., and a male of Akodon orophilus. Thomasomys sp. had a karyotype of 2n = 42, XY (n = 21, meanwhile A. orophilus presented 2n = 22, XY (n = 11. Comparisons between chromosomal pairs from the existent literature indicate that both are new karyotypes. Thomasomys sp. has a distinct sexual Y chromosome, the only metacentric (m reported for the genus. The chromosomes X and Y of A. orophilus are acrocentrics (a; and the length of chromosome Y (2/3 of the length of X distinguishes A. orophilus from other congeneric. Because the structural differences between the sexual chromosomes usually generates mechanism of reproductive isolation at intraspecific level and are bigger still in interspecific crosses, we concluded that the karyotypes reported here support the validity of the species A. orophilus and suggest that Thomasomys sp. represents a new species to science.

  12. Enraizamiento in vitro de Dioscoreas sp. In vitro rootting of Dioscoreas sp.

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    Jarma Alfredo

    2003-12-01

    Full Text Available El presente estudio se realizó en el Laboratorio de Cultivo de Tejidos Vegetales de la Universidad de Córdoba en el año 2000 con el objetivo de evaluar el efecto del ácido naftalenacético (ANA en el medio de cultivo sobre el enraizamiento in vitro de tres cultivares de ñame (Dioscorea sp.. Se estudió el efecto de cuatro concentraciones del regulador de crecimiento (0, 0.3, 0.6 y 0.9 mg/1 sobre tres cultivares de ñame (Diamantes-22, 003 y 005. Se empleó un experimento trifactorial con diseño aleatorio y 20 repeticiones; cada unidad experimental estuvo conformada por un recipiente de vidrio que contenía el medio de cultivo y el explante (segmento nodal. Las variables consideradas fueron número y grosor de raíces, oxidación del medio de cultivo y producción de callo. Los resultados indicaron que tanto la hormona como el genotipo tuvieron efecto sobre todas las variables consi­deradas en el estudio y que la interacción fue importante (PThe Universidad de Córdoba s Vegetal Tissue Culture Laboratory evaluated the effect of naphthalenacetic acid (NAA on in vitro rooting of three yam cultivars (Dioscorea sp. in 2000. The effect of four hormone levels (0, 0.3, 0.6 and 0.9 mg/1 was studied on three yam cultivars (Diamantes-22,003 and 005. A random experimental design was used employing 4x3 factorial arrangement and 20 repetitions; each experimental unit consisted of a glass receptacle containing the culture medium and the explant (one segment nodal. The variables considered were the number of roots and their thickness, culture medium oxidation and callus production. Findings showed that both the hormone and genotype had an effect on all those variables considered in the study and interaction was significant (P <0.05 for callus production and culture medium oxidation. The 005 cultivar showed the greatest root number and thickness values. It was also determined that when a 0.6 and 0.9 mg/1 dose of auxin was applied, root production and

  13. Further brominated bis- and tris-indole alkaloids from the deep-water New Caledonian marine sponge Orina Sp.

    Science.gov (United States)

    Bifulco, G; Bruno, I; Riccio, R; Lavayre, J; Bourdy, G

    1995-08-01

    Two tris-indole alkaloids, (+/-) gelliusines A and B [1], have been isolated for the first time from a marine source, the New Caledonian sponge, Orina sp. (or Gellius sp.), along with five further indole constituents [2-6]. Compound 6 has been identified as 2,2-bis-(6'-bromo-3'-indolyl(-ethylamine, previously isolated from the tunicate Didemnum candidum, but the remaining four indoles [2-5] are novel compounds. These showed anti-serotonin activity and a strong affinity for somatostatin and neuropeptide Y receptors in receptor-binding assays.

  14. Eficiencia de pseudomonas sp, rhodopseudomonas sp, micrococcus sp y bacillus sp empleados como cultivos individuales y en consorcio, en la degradación de petróleo diesel ii

    OpenAIRE

    Otiniano García, Nélida Milly Esther

    2010-01-01

    In order to evaluate the efficiency of Pseudomonas sp, Rhodopseudomonas sp, Micrococcus sp, Bacillus sp, and the consortium formed by these four microorganisms in the diesel II petroleum degradation, it was worked in 5 bioreactors of aerated and shaken tank of 1.5 litters of capacity, with speed agitation of 120 rpm, and air flow of 0.5 vvm; in which were placed; 940 mL of Minimum Broth of Davies pH 7.0; 50 mL of diesel II petroleum as source of carbon and 10 mL of a suspension of approx...

  15. Biodegradation of phenol and benzene by endophytic bacterial strains isolated from refinery wastewater-fed Cannabis sativa.

    Science.gov (United States)

    Iqbal, Aneela; Arshad, Muhammad; Hashmi, Imran; Karthikeyan, Raghupathy; Gentry, Terry J; Schwab, Arthur Paul

    2017-06-13

    The presence of benzene and phenol in the environment can lead to serious health effects in humans and warrant development of efficient cleanup strategies. The aim of the present work was to assess the potential of indigenous endophytic bacterial strains to degrade benzene and phenol. Seven strains were successfully isolated from Cannabis sativa plants irrigated with oil refinery wastewater. Molecular characterization was performed by 16S rRNA gene sequencing. Phenol was biodegraded almost completely with Achromobacter sp. (AIEB-7), Pseudomonas sp. (AIEB-4), and Alcaligenes sp. (AIEB-6) at 250, 500, and 750 mg L -1 ; however, the degradation was only 81%, 72%, and 69%, respectively, when exposed to 1000 mg L -1 . Bacillus sp. (AIEB-1), Enterobacter sp. (AIEB-3), and Acinetobacter sp. (AIEB-2) degraded benzene significantly at 250, 500, and 750 mg L -1 . However, these strains showed 80%, 72%, and 68% benzene removal at 1000 mg L -1 exposure, respectively. Rates of degradation could be modeled with first-order kinetics with rate constant values of 1.86 × 10 -2 for Pseudomonas sp. (AIEB-4) and 1.80 × 10 -2  h -1 for Bacillus sp. (AIEB-1) and half-lives of 1.5 and 1.6 days, respectively. These results establish a foundation for further testing of the phytoremediation of hydrocarbon-contaminated soils in the presence of these endophytic bacteria.

  16. Phylogenetic analysis of Tomato mosaic virus from Hemerocallis sp. and Impatiens hawkeri Análise filogenética de Tomato mosaic virus isolado de Hemerocallis sp. e Impatiens hawkeri

    Directory of Open Access Journals (Sweden)

    Lígia Maria Lembo Duarte

    2007-12-01

    , respectivamente, assim como estabelecer relações filogenéticas entre os isolados e outras espécies de Tobamovirus, foram realizados extrações do RNA, RT-PCR, seqüenciamento do gene da capa protéica e análises filogenéticas. Quando foram comparadas seqüências homólogas de tobamovirus, valores superiores a 98,5% de identidade, ao nível de nucleotídeos, foram obtidos com isolados do Tomato mosaic virus (ToMV. Em relação ao tobamo-H, foi encontrado 100% de identidade com o ToMV isolado de tomateiro da Austrália e do Peru. Com base em análise de máxima verossimilhança (MV, sugere-se que o tobamo-H e tobamo-I compartilham um ancestral comum com ToMV, Tobacco mosaic virus, Odontoglossum ringspot virus e Pepper mild mottle virus. A topologia da árvore reconstruída a partir de MV mostra um grupo monofilético, sustentado por 100% de "bootstrap", formado por vários isolados de ToMV de diferentes hospedeiras, incluindo ornamentais, a partir de diferentes localizações geográficas. Os resultados indicam que Hemerocallis sp.e I. hawkeri estão infectados pelo ToMV, sendo esse o primeiro relato desse vírus em ornamentais, no Brasil.

  17. Inhibitory effects of silver zeolite on in vitro growth of fish egg pathogen, Saprolegnia sp.

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    Seyed Ali Johari

    2014-05-01

    Full Text Available Objective: To investigate the effects of powdered silver zeolite (SZ on the in vitro growth of the fish pathogen Saprolegnia sp. Methods: The antifungal activity of SZ was evaluated by determining the minimum inhibitory concentrations using two-fold serial dilutions of powdered SZ in a glucose yeast extract agar at 22 °C. The growth of Saprolegnia sp. on the SZ agar treatments was compared to that on SZ-free agar controls. Results: The results showed that SZ had an inhibitory effect on the in vitro growth of the tested fungi. The minimum inhibitory concentration of SZ for Saprolegnia sp. was also calculated at 600 mg/L, which is equal to 0.06 percent. Conclusions: SZ is a potential good candidate to replace teratogenic and toxic agents, such as malachite green in aquaculture systems.

  18. Robo-AO KP: A new era in robotic adaptive optics

    Science.gov (United States)

    Riddle, Reed L.; Baranec, Christoph; Law, Nicholas M.; Kulkarni, Shrinivas R.; Duev, Dmitry; Ziegler, Carl; Jensen-Clem, Rebecca M.; Atkinson, Dani Eleanor; Tanner, Angelle M.; Zhang, Celia; Ray, Amy

    2016-01-01

    Robo-AO is the first and only fully automated adaptive optics laser guide star AO instrument. It was developed as an instrument for 1-3m robotic telescopes, in order to take advantage of their availability to pursue large survey programs and target of opportunity observations that aren't possible with other AO systems. Robo-AO is currently the most efficient AO system in existence, and it can achieve an observation rate of 20+ science targets per hour. In more than three years of operations at Palomar Observatory, it has been quite successful, producing technology that is being adapted by other AO systems and robotic telescope projects, as well as several high impact scientific publications. Now, Robo-AO has been selected to take over operation of the Kitt Peak National Observatory 2.1m telescope. This will give Robo-AO KP the opportunity to pursue multiple science programs consisting of several thousand targets each during the three years it will be on the telescope. One-sixth of the observing time will be allocated to the US community through the NOAO TAC process. This presentation will discuss the process adapting Robo-AO to the KPNO 2.1m telescope, the plans for integration and initial operations, and the science operations and programs to be pursued.

  19. Adesão ao tratamento da ambliopia

    Directory of Open Access Journals (Sweden)

    Arakaki Mara Regina

    2004-01-01

    Full Text Available OBJETIVO: Avaliar a influência da adesão no resultado do tratamento da ambliopia. MÉTODOS: Foi feito estudo retrospectivo avaliando a resposta ao tratamento da ambliopia em 151 crianças portadoras de ambliopia, tratadas com esquema de oclusão diária, com o número de dias variando de acordo com a idade da criança. Os pacientes foram separados em dois grupos que diferiam entre si pela adesão ou não ao tratamento. RESULTADOS: Observou-se que o tratamento teve melhores resultados nas crianças de 4 a 7 anos e que aderiram ao tratamento; as crianças que aderiram ao tratamento necessitaram de tempo menor de permanência em tratamento. Mesmo crianças com idade superior a 7 anos tiveram resultados positivos com a oclusão. CONCLUSÃO:O tratamento oclusivo é um meio terapêutico eficiente desde que haja a cooperação do paciente.

  20. Tamanho ao nascer e problemas de saúde mental aos 11 anos em uma coorte brasileira de nascimentos

    Directory of Open Access Journals (Sweden)

    Erika Alejandra Giraldo Gallo

    2011-08-01

    Full Text Available O objetivo foi avaliar a associação entre tamanho ao nascer e problemas de saúde mental aos 11 anos na Coorte de Nascimentos de Pelotas, Rio Grande do Sul, Brasil, de 1993. Foram pesados e medidos ao nascer 4.358 recém-nascidos. Avaliou-se problemas de saúde mental com o questionário de capacidades e dificuldades (Strengths and Difficulties Questionnaire - SDQ. A prevalência de problemas de saúde mental foi de 32% (IC95%: 31-33. Na análise ajustada, os 291 (6,7% recém-nascidos com escorez de peso/idade e os 268 (6,2% com índice de massa corporal (IMC/idade +2 DP tiveram, respectivamente, 34% (IC95%: 6-71 e 19% (IC95%: 1-40 maior risco de apresentar esses problemas se comparados com aqueles com escore normal. Os resultados sugerem que fatores ocorridos na gestação e refletidos nas medidas de tamanho ao nascer podem ocasionar problemas de saúde mental em etapas tardias.

  1. Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium oxysporum f.sp. cubense dengan Trichoderma sp.

    Directory of Open Access Journals (Sweden)

    Albertus Sudirman

    2011-07-01

    Full Text Available The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag, second factor was time of Trichoderma culture application (2 weeks before Foc inoculation, at same time with Foc inoculation and 2 weeks after Foc inoculation. Trichoderma sp. was cultured in mixed rice brand and chaff medium. The disease intensity was observed with scoring system of wilting leaves (0–4. The results showed that Trichoderma sp. was antagonistic against Foc in vitro and inhibited 86% of Foc colony development. Mechanism of antagonism between Trichoderma sp. and Foc was hyperparasitism. Trichoderma hyphae coiled around Foc hyphae. Lysis of Foc hyphae was occurred at the attached site of Trichoderma hyphae on Foc hyphae. Added banana seedling with Trichoderma sp. Culture reduced disease intensity of Fusarium wilt. Suggested dose of Trichoderma culture application in glass house was 25 g/polybag, given at the same time with Foc inoculation. Penelitian ini bertujuan untuk mengetahui kemampuan Trichoderma sp. untuk pengendalian penyakit layu fusarium pisang di rumah kaca. Penelitian meliputi pengujian daya hambat Fusarium oxysporum f.sp. cubense (Foc in vitro dan kemampuan menekan intensitas penyakit di rumah kaca. Penelitian in vitro meliputi uji antagonisme dan mekanismenya yang dilakukan secara dual culture. Uji pengaruh Trichoderma sp. terhadap penyakit layu Fusarium dilakukan di rumah kaca dengan Rancangan Acak Lengkap Faktorial. Faktor pertama adalah dosis biakan Trichoderma sp., dengan tiga aras (0, 25, 50 g/per bibit dalam polibag. Faktor kedua

  2. Induced abortion is not associated with a higher likelihood of depression in Curaçao women.

    Science.gov (United States)

    Boersma, Adriana A; van den Berg, Desirée; van Lunsen, Rik H W; Laan, Ellen T M

    2014-10-01

    To investigate the risk of developing a depression after induced abortion. A prospective cohort study conducted in Curaçao which involved 92 women having an induced abortion and 37 women delivering after an unplanned or unwanted pregnancy, who served as controls. All participants completed the Center of Epidemiological Studies Depression (CES-D) scale before and two to three weeks after the abortion or delivery. Following the abortion, significantly fewer women were at risk of depression (30%) as compared to when still pregnant (60%). Mean depression scores were significantly lower after- than before the procedure. The likelihood of depression post-abortum (30%) was similar to that after delivery of an unplanned/unwanted child (22%). Even though women in the abortion group more often reported having suffered from depression in the past than controls, they were not at greater risk of depression after their pregnancy had ended. Curaçao women's risk of developing a depression following an (early) induced abortion is not greater than that after carrying to term an unplanned/unwanted pregnancy. We recommend that the results of this study be taken into account in case the Curaçao government should consider legalisation of induced abortion in the near future.

  3. Special offer

    CERN Multimedia

    Staff Association

    2011-01-01

    OFFRE SPECIALE POUR NOS MEMBRES Les vendredis 29 juillet, 5 et 12 août, Aquaparc fermera ses portes exceptionnellement à 22h00. Pour ces évènements, des tarifs défiant toute concurrence vous sont proposés. Au programme : Clown spécialiste de la sculpture de ballons de 16h00 à 21h00 Ambiance Salsa avec danseurs professionnel : Démonstration et Cours de Salsa. Les tarifs : Pour une entrée à partir de 15h00 : Enfant : CHF 22.- Adulte : CHF 26.-  

  4. Pesquisa de Acinetobacter sp e Pseudomonas aeruginosa produtores de metalo-β-lactamase em hospital de emergência de Porto Alegre, Estado do Rio Grande do Sul, Brasil Investigation of metallo-β-lactamase-producing Acinetobacter sp and Pseudomonas aeruginosa at an emergency hospital in Porto Alegre, State of Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Vani Dos Santos Laranjeira

    2010-08-01

    Full Text Available INTRODUÇÃO: O aparecimento de Pseudomonas aeruginosa e Acinetobacter sp produtores de metalo-β-lactamases (MBLs é um desafio para os hospitais. MÉTODOS: Verificou-se a produção de MBL em cepas clínicas de Pseudomonas aeruginosa e Acinetobacter sp de um hospital de emergência de Porto Alegre pelo método de aproximação de disco e E-test MBL. Os genes bla foram pesquisados pela PCR. RESULTADOS: Duas cepas de Pseudomonas aeruginosa e oito Acinetobacter sp demonstraram fenótipo de MBLs. A amplificação do gene blaSPM-1 confirmou a enzima em P. aeruginosa.. CONCLUSÕES: Deve-se ter cautela ao avaliar testes fenotípicos utilizados na detecção rotineira de metalo-enzima.INTRODUCTION: The appearance of metallo-β-lactamase (MBL-producing Pseudomonas aeruginosa and Acinetobacter sp. is a challenge for hospitals. METHODS: The production of MBL in clinical isolates of Pseudomonas aeruginosa and Acinetobacter sp. From an emergency hospital in Porto Alegre was investigated using the disk approximation test and MBL E-test. The bla genes were determined using PCR. RESULTS: Two strains of Pseudomonas aeruginosa and eight of Acinetobacter sp were shown to be MBL phenotypes. Amplification of the blaSPM-1 gene confirmed the presence of the enzyme in P. aeruginosa. CONCLUSIONS: Caution is needed in evaluating phenotype tests used for routine detection of metallo-β-lactamases.

  5. SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis

    Directory of Open Access Journals (Sweden)

    Mario Saare

    2015-01-01

    Full Text Available The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member SP140L. The genome sequence analysis showed the formation of SP140L gene through rearrangements of the two neighboring genes, SP100 and SP140, during the evolution of higher primates. The SP140L expression is interferon-inducible with high transcript levels in B cells and other peripheral blood mononuclear cells. Subcellularly, SP140L colocalizes with SP100 and SP140 in nuclear structures that are devoid of SP110, PML, or p300 proteins. Similarly to SP100 and SP140 protein, we detected serum autoantibodies to SP140L in patients with primary biliary cirrhosis using luciferase immunoprecipitation system and immunoblotting assays. In conclusion, our results show that SP140L is phylogenetically recent member of SP100 proteins and acts as an autoantigen in primary biliary cirrhosis patients.

  6. Resolution of alpha/beta-amino acids by enantioselective penicillin G acylase from Achromobacter sp

    Czech Academy of Sciences Publication Activity Database

    Grulich, Michal; Brezovský, J.; Štěpánek, Václav; Palyzová, Andrea; Kyslíková, Eva; Kyslík, Pavel

    2015-01-01

    Roč. 122, DEC 2015 (2015), s. 240-247 ISSN 1381-1177 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:61388971 Keywords : Penicillin G acylase * Enantioselectivity * Homologous model Subject RIV: CE - Biochemistry Impact factor: 2.189, year: 2015

  7. Geoecologia da paisagem X legislação ambiental: uma análise da distribuição espacial das restrições ao uso da terra frente à problemática erosiva na alta bacia do Ribeirão Areia Dourada, Marabá Paulista (SP / Geoecology of the landscape X environmental legislation: an analysis of the spatial distribution of the land use restrictions and ther relation with erosion issues in the upper basin of the river Areia Dourada, Marabá Paulista (SP

    Directory of Open Access Journals (Sweden)

    Felipe Augusto Scudeller Zanatta

    2016-09-01

    Full Text Available A promulgação do atual Código Florestal (Lei 12.651/12 gerou preocupações quanto à preservação e à proteção dos recursos naturais nos diferentes Biomas do Brasil, muito devido ao seu caráter genérico e à tolerância aos usos abusivos. Frente a essa questão, neste artigo é apresentado um estudo aplicado e comparativo das restrições ao uso nas propriedades privadas de acordo o atual Código Florestal, e com o zoneamento ambiental, elaborado a partir da proposta da geoecologia da paisagem, aplicados na alta bacia do ribeirão Areia Dourada, Marabá Paulista (SP. Assim, tem-se como objetivo avaliar as diferenças espaciais na restrição ao uso da terra identificadas em consonância com a Lei 12.651/12 e com o estudo conduzido a partir da metodologia da geoecologia, que se caracteriza por integrar os diversos elementos que compõem a paisagem natural. A demais, foram acrescidas à análise as feições erosivas lineares, no intuito de comparar a fragilidade natural da bacia com as consequências de usos inadvertidos. Como resultado, o atual Código Florestal contempla a preservação de apenas 6,28% (290,55 ha da área da bacia, o que tende a agravar a problemática erosiva, enquanto a metodologia da geoecologia aponta para a necessidade de preservar, pelo menos, 51,66% (2.388,28 ha. Esses terrenos ainda incidem diretamente nas áreas com erosões em ravinas e voçorocas, indicando de forma eficiente a fragilidade natural da bacia.

  8. Jogos de Empresas: Abordagens ao Fenômeno, Perspectivas Teóricas e Metodológicas

    Directory of Open Access Journals (Sweden)

    Valdete de Oliveira Mrtvi

    2017-01-01

    Full Text Available Os jogos de empresas, além de utilizados no processo de ensino, também são explorados como ferramentas de pesquisa, conforme Gentry, Tice, Robertson e Gentry (1984. Este estudo, de natureza qualitativa e quantitativa, descritivo quanto aos objetivos, e caracterizado como revisão integrativa no tocante ao método, trata do seguinte problema de pesquisa: Qual o perfil da pesquisa acadêmica brasileira sobre jogos de empresas considerando a abordagem ao fenômeno e as perspectivas teóricas e metodológicas? Foram analisados 226 artigos extraídos de periódicos online, do indexador Spell e anais de eventos. Como resultado, é proposta uma tipologia para classificação das pesquisas em jogos de empresas, considerando a abordagem ao fenômeno de pesquisa e os eixos temáticos. Constatou-se a predominância de estudos que têm as simulações como objeto de pesquisa - SCOPE (77,9% -, enquanto 22,1% utilizam os jogos de empresas como ambiente de pesquisa - SCAPE. Verificou-se que 38,5% dos estudos não mencionam quaisquer das classificações metodológicas analisadas. Discute-se algumas fragilidades metodológicas identificadas nas pesquisas da área. Apesar de as classificações não serem determinantes para a qualidade de um estudo, representam importante indicador da consistência do processo de reflexão e da coesão da investigação.

  9. Environmental conditions affecting exopolysaccharide production by Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.

    Science.gov (United States)

    Kiliç, Nur Koçberber; Dönmez, Gönül

    2008-06-15

    Three different chromium-resistant microorganisms (Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.) were tested with regard to their EPS production at different pH levels, temperatures, Cr(VI) concentrations, and incubation periods. The optimum pH level was 7 for P. aeruginosa and Micrococcus sp., while it was 8 for Ochrobactrum sp. according to the highest EPS amount at 100 mg/L Cr(VI) concentration. The highest production of EPSs by the three bacteria was obtained under different environmental conditions. P. aeruginosa produced the highest EPS (863.3 mg/L) after incubation for 96 h on media with 50 mg/L Cr(VI) at 20 degrees C, Micrococcus sp. gave the highest yield (444.6 mg/L) after incubation for 72 h on media with 100 mg/L Cr(VI) at the same temperature, and Ochrobactrum sp. had the highest production (430.5 mg/L) on media with 150 mg/L Cr(VI) at 30 degrees C at the end of 48 h of incubation.

  10. Superhard sp2–sp3 hybrid carbon allotropes with tunable electronic properties

    Directory of Open Access Journals (Sweden)

    Meng Hu

    2016-05-01

    Full Text Available Four sp2–sp3 hybrid carbon allotropes are proposed on the basis of first principles calculations. These four carbon allotropes are energetically more favorable than graphite under suitable pressure conditions. They can be assembled from graphite through intralayer wrinkling and interlayer buckling, which is similar to the formation of diamond from graphite. For one of the sp2–sp3 hybrid carbon allotropes, mC24, the electron diffraction patterns match these of i-carbon, which is synthesized from shock-compressed graphite (H. Hirai and K. Kondo, Science, 1991, 253, 772. The allotropes exhibit tunable electronic characteristics from metallic to semiconductive with band gaps comparable to those of silicon allotropes. They are all superhard materials with Vickers hardness values comparable to that of cubic BN. The sp2–sp3 hybrid carbon allotroes are promising materials for photovoltaic electronic devices, and abrasive and grinding tools.

  11. Selective C(sp2)-C(sp) bond cleavage: the nitrogenation of alkynes to amides.

    Science.gov (United States)

    Qin, Chong; Feng, Peng; Ou, Yang; Shen, Tao; Wang, Teng; Jiao, Ning

    2013-07-22

    Breakthrough: A novel catalyzed direct highly selective C(sp2)-C(sp) bond functionalization of alkynes to amides has been developed. Nitrogenation is achieved by the highly selective C(sp2)-C(sp) bond cleavage of aryl-substituted alkynes. The oxidant-free and mild conditions and wide substrate scope make this method very practical. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. In vitro cytotoxicity evaluation of nano-carbon particles with different sp{sup 2}/sp{sup 3} ratios

    Energy Technology Data Exchange (ETDEWEB)

    Li, S.S.; Wu, B.J.; Deng, Q.Y. [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China); Guo, Y.B. [The Third People' s Hospital of Chengdu, Sichuan 610031 (China); Leng, Y.X., E-mail: yxleng@263.net [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China); Huang, N. [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China)

    2017-06-01

    Graphitization occurs during the long-term service of a diamond-like carbon (DLC) modified artificial joint. Then, DLC wear debris, which are carbon particles with different sp{sup 2}/sp{sup 3} ratios and sizes ranging from the nano- to micro-meter scale produced. In this paper, to promote the application of DLC coating for artificial joint modification, the cytotoxicity of DLC debris (nano-carbon particles, NCs) with different sp{sup 2}/sp{sup 3} ratios was studied. The microstructure and physical characteristics of NCs with different sp{sup 2}/sp{sup 3} ratios were investigated by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Transmission Electron Microscope (TEM) and Dynamic Light Scattering (DLS). Meanwhile, osteoblasts and macrophages were applied to characterize the cytotoxicity of the NCs. In vitro cytotoxicity assay results indicated that cells incubated with NCs of different sp{sup 2}/sp{sup 3} ratios had greater osteogenic capacity, and these particles caused a weaker immune response in comparison with CoCrMo particles. Taken together, the results indicated that NCs with different sp{sup 2}/sp{sup 3} ratios presented a good cytocompatibility than CoCrMo particles. But no significant differences were observed among NCs with different sp{sup 2}/sp{sup 3} ratios. The better cytocompatibility of NCs is mainly attributable to their surface charge. - Highlights: • NCs with different sp{sup 2}/sp{sup 3} ratios have been successfully prepared by annealing treatment. • NCs with different sp{sup 2}/sp{sup 3} ratios show good osteogenic capacity and lower immune response. • The good cytocompatibility of NCs is mainly dependent on its surface charge.

  13. Cultivation of Nannochloropsis sp. in brackish groundwater supplemented with municipal wastewater as a nutrient source

    Directory of Open Access Journals (Sweden)

    Louise Lins de Sousa

    2014-04-01

    Full Text Available The aim of this work was to study growth potential of the green microalgae Nannochloropsis sp. using brackish groundwater from a well in the semi-arid northeast region of Brazil as culture medium. The medium was supplemented with (% 19.4, 22.0, 44.0 and 50.0% of municipal wastewater after UASB treatment as a low-cost nutrient source. The results showed that the culture tested was capable of growing in the brackish groundwater even at salinity levels as low as 2 ppt. Furthermore it was shown that municipal wastewater could be used as a sole nutrient source for Nannochloropsis sp.

  14. Xylanolytic enzyme systems in Arthrobacter sp MTCC 5214 and Lactobacillus sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Khandeparker, R.; Jalal, T.

    The production of extracellular xylanolytic enzymes such as xylanase, alfa-L-arabinofuranosidase (alfa-l-AFase), and acetyl xylan esterase (Axe) by marine Arthrobacter sp and Lactobacillus sp was investigated using different carbon sources Induction...

  15. Eimeria pipistrellus n. sp. from Pipistrellus kuhlii (Chiroptera: Vespertilionidae) in Saudi Arabia

    Science.gov (United States)

    Al-Dakhil, Mohamed; Al-Shawa, Yaser

    1999-01-01

    Fecal samples from 12 Pipistrellus kuhlii captured at Shagrah, Saudi Arabia, were examined for coccidia and three (25%) found to harbor a undescribed eimerian, herein described as Eimeria pipistrellus n. sp. Sporulated oocysts were subspherical, 24.8×23.2 (22-27×20-25) µm, with a bilayered and smooth wall. The micropyle was absent, but a large oocyst residuum and a single polar granule were present. Sporocysts were ovoid, 11.6×8.3 (10.5-13×7.5-9) µm, with a prominent Stieda body, but without a substiedal body; sporozoites lay head to tail in sporocysts and contained one large posterior refractile body. Eimeria pipistrellus n. sp. is the 3rd species of the genus Eimeria found from bats of the genus Pipistrellus. PMID:10188376

  16. Research on AO/FO batch management technology in aircraft production

    Directory of Open Access Journals (Sweden)

    Yin Haijun

    2018-01-01

    Full Text Available Based on the analysis of the characteristics and significance of AO/FO in the process of aircraft production, this paper analyzes the format rules of AO/FO batch management from the perspective of technology realization, and details the AO/FO The change of the query and the change status tracking, introduces the AO/FO single-stand status display in the batch management, increases the structure definition of the attribute table in the batch management, and designs the relevant algorithm to store and calculate the batch information. Finally, based on the above theory support AO/FO batch management system successfully used in the production of a machine.

  17. Purification and Partial characterization of manganese peroxidase from Bacillus pumilus AND Paenibacillus sp.

    Directory of Open Access Journals (Sweden)

    Patrícia Lopes de Oliveira

    2009-12-01

    Full Text Available The production of manganese peroxidase (MnP from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried out with the purified MnP. The maximum activity reached for B. pumilus and Paenibacillus sp. MnPs were 4.3 U/L at pH 8.0 and 25 ºC and 11.74 U/L at pH 9.0 and 35 ºC, respectively. The molar masses determined by SDS-PAGE gel eletrophoresis were 25 kDa and 40 kDa, respectively, for the purified enzyme from B. pumilus and Paenibacillus sp.

  18. Sensibilidade da rosa 'Osiana' ao etileno

    Directory of Open Access Journals (Sweden)

    Delaine Cristina Cordeiro

    2011-01-01

    Full Text Available Os objetivos deste trabalho foram determinar a sensibilidade e as respostas da rosa cultivar Osiana (Rosa × hybrida à presença de etileno ao longo da vida pós-colheita. Para determinar a sensibilidade da flor ao etileno, hastes foram cortadas e colocadas em câmaras herméticas e em seguida tratadas por 24 horas com diferentes concentrações de etileno variando de 0,0; 0,1; 1,0; 10; 100 e 1000 µL L-1do ar no interior da câmara. A rosa teve a vida pós-colheita reduzida pelo etileno, quando exposta a concentração igual ou superior a 10 µL L-1 de etileno. As flores tratadas com no mínimo de 10 µL L-1de etileno desenvolveram necrose, murcha e abscisão prematura das pétalas ainda túrgidas. As concentrações de 0,1 e 1,0 µL L-1de etileno estimularam a abertura do botão floral durante o período de 24 horas de exposição ao etileno, sem no entanto, reduzir a vida pós-colheita. A rosa 'Osiana' pode ser classificada como uma cultivar sensível ao etileno e a concentração influência os processos estimulados.

  19. Sp6 and Sp8 Transcription Factors Control AER Formation and Dorsal-Ventral Patterning in Limb Development

    Science.gov (United States)

    Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C.; Ros, Marian A.

    2014-01-01

    The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6−/−;Sp8+/−) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/βcatenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

  20. Pacientes assintomáticos apresentam infecção relacionada ao cateter venoso utilizado para terapia nutricional parenteral Asymptomatic patients present infection related to the central venous catheter used for total parenteral nutrition

    Directory of Open Access Journals (Sweden)

    Juliana Deh Carvalho Machado

    2009-12-01

    Full Text Available OBJETIVO: Avaliar a freqüência de infecção relacionada ao cateter venoso central em pacientes submetidos a terapia nutricional parenteral. MÉTODOS: Foram analisados os cateteres venosos centrais de pacientes em terapia nutricional parenteral que tiveram a indicação de retirada do cateter venoso central por infecção, alta hospitalar, ou trombose. Os pacientes com infecção foram denominados de Grupo 1 e os demais de Grupo 2. RESULTADOS: Não houve diferença estatisticamente significante quanto ao estado nutricional dos 18 pacientes analisados. Foram analisados 28 cateteres e destes 68% estavam infectados, sendo 72% do Grupo 1 e 28% do Grupo 2 (assintomáticos. No Grupo 1, houve infecção sistêmica em 70% dos casos, já no Grupo 2 a hemocultura foi positiva em 17% dos casos. A colonização por Staphylococcus sp. ocorreu em 48% dos casos, seguida de Candida sp. (21%, Enterococcus faecalis (16%, Pseudomonas aerurginosa (10% e Proteus sp.(5%. CONCLUSÃO: A contaminação de cateter venoso central utilizado para terapia nutricional parenteral é freqüente. Mesmo pacientes assintomáticos recebendo nutrição parenteral têm uma incidência maior de infecção por Candida sp. Portanto é necessária a criação de barreiras que impeçam a colonização destes cateteres venosos centrais, a fim de diminuir a morbimortalidade de pacientes dependentes deste tipo de terapia.OBJECTIVE: The aim of this study was to evaluate the frequency of central venous catheter-related infections in hospitalized patients receiving total parenteral nutrition. METHODS: Central venous catheters were analyzed immediately after removal due to infection, hospital discharge or thrombosis. The patients with catheter-related infection were named Group 1 and the other patients were named Group 2. RESULTS: Eighteen patients were studied. There was no statistically significant difference in nutritional status between the two groups. A total of 28 catheters were analyzed

  1. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource

    OpenAIRE

    Srivastava, Akanksha; Tiwari, Ratnakar; Srivastava, Vikas; Singh, Tej Bali; Asthana, Ravi Kumar

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for ant...

  2. Polarization burst in the BL Lac object AO 0235 + 164

    Energy Technology Data Exchange (ETDEWEB)

    Impey, C D; Brand, P W.J.L. [Edinburgh Univ. (UK). Dept. of Astronomy; Tapia, S [Steward Observatory, Tucson, AZ (USA)

    1982-01-01

    Simultaneous infrared and optical polarimetry and photometry have been obtained for AO 0235 + 164 covering a five night period. The object underwent a polarization burst during which the 2.2 ..mu..m polarization rose from 17.5 to 28.7 per cent and fell again to 14.9 per cent. At its peak the degree of optical polarization was 43.9 per cent, the highest linear polarization observed in a BL Lac object. The data show the degree of polarization to increase towards shorter wavelengths, and the effect is inconsistent with either dilution by a galactic component or simple one-component synchrotron models. The large changes in polarization are not accompanied by large changes in flux, a result which is difficult to explain using conventional models of these objects. Other implications of the luminosity, polarization and variability are discussed.

  3. Mycobacterium lutetiense sp. nov., Mycobacterium montmartrense sp. nov. and Mycobacterium arcueilense sp. nov., members of a novel group of non-pigmented rapidly growing mycobacteria recovered from a water distribution system.

    Science.gov (United States)

    Konjek, Julie; Souded, Sabiha; Guerardel, Yann; Trivelli, Xavier; Bernut, Audrey; Kremer, Laurent; Welte, Benedicte; Joyeux, Michel; Dubrou, Sylvie; Euzeby, Jean-Paul; Gaillard, Jean-Louis; Sapriel, Guillaume; Heym, Beate

    2016-09-01

    From our recent survey of non-pigmented rapidly growing mycobacteria in the Parisian water system, three groups of isolates (taxons 1-3) corresponding to possible novel species were selected for taxonomic study. The three taxa each formed creamy white, rough colonies, had an optimal growth temperature of 30 °C, hydrolyzed Tween 80, were catalase-positive at 22 °C and expressed arylsulfatase activity. All three were susceptible to amikacin, ciprofloxacin and tigecycline. The three taxa produced specific sets of mycolic acids, including one family that has never previously been described, as determined by thin layer chromatography and nuclear magnetic resonance. The partial rpoB sequences (723 bp) showed 4-6 % divergence from each other and more than 5 % differences from the most similar species. Partial 16S rRNA gene sequences showed 99 % identity within each species. The most similar sequences for 16S rRNA genes (98-99 % identity over 1444-1461 bp) were found in the Mycobacterium fortuitum group, Mycobacterium septicum and Mycobacterium farcinogenes. The three taxa formed a new clade (bootstrap value, 99 %) on trees reconstructed from concatenated partial 16S rRNA, hsp65 and rpoB sequences. The above results led us to propose three novel species for the three groups of isolates, namely Mycobacterium lutetiense sp. nov. [type strain 071T=ParisRGMnew_1T (CIP 110656T=DSM 46713T)], Mycobacterium montmartrense sp. nov. [type strain 196T=ParisRGMnew_2T (CIP 110655T=DSM 46714T)] and Mycobacteriu marcueilense sp. nov. [type strain of 269T=ParisRGMnew_3T (CIP 110654T=DSM 46715T)].

  4. Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls.

    Science.gov (United States)

    Nishida, Yasuhiro; Adachi, Kyoko; Kasai, Hiroaki; Shizuri, Yoshikazu; Shindo, Kazutoshi; Sawabe, Akiyoshi; Komemushi, Sadao; Miki, Wataru; Misawa, Norihiko

    2005-08-01

    A carotenoid biosynthesis gene cluster mediating the production of 2-hydroxyastaxanthin was isolated from the marine bacterium Brevundimonas sp. strain SD212 by using a common crtI sequence as the probe DNA. A sequence analysis revealed this cluster to contain 12 open reading frames (ORFs), including the 7 known genes, crtW, crtY, crtI, crtB, crtE, idi, and crtZ. The individual ORFs were functionally analyzed by complementation studies using Escherichia coli that accumulated various carotenoid precursors due to the presence of other bacterial crt genes. In addition to functionally identifying the known crt genes, we found that one (ORF11, named crtG) coded for a novel enzyme, carotenoid 2,2'-beta-hydroxylase, which showed intriguingly partial homology with animal sterol-C5-desaturase. When this crtG gene was introduced into E. coli accumulating zeaxanthin and canthaxanthin, the resulting transformants produced their 2-hydroxylated and 2,2'-dihydroxylated products which were structurally novel or rare xanthophylls, as determined by their nuclear magnetic resonance and high-performance liquid chromatography/photodiode array detector/atmospheric pressure chemical ionization mass spectrometry spectral data. The new carotenoid produced was suggested to have a strong inhibitory effect on lipid peroxidation.

  5. Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium Oxysporum F.sp. Cubense) dengan Trichoderma SP.

    OpenAIRE

    Sudirman, Albertus; Sumardiyono, Christanti; Widyastuti, Siti Muslimah

    2011-01-01

    The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc) in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag), second...

  6. The Effect of Acupuncture to SP6 on Skin Temperature Changes of SP6 and SP10: An Observation of “Deqi”

    Directory of Open Access Journals (Sweden)

    Jia-Min Yang

    2014-01-01

    Full Text Available Background. Deqi sensation is a complex but an important component for acupuncture effect. In this study, we tried to observe the relationship between Deqi and skin temperature changes and whether there was some relativity between Deqi and needle stimulations on cold congealing and dysmenorrhea rat model. Thirty-two female Sprague Dawley (SD rats were randomly divided into four groups (Saline Control Group, Model Group, Group A with strong stimulation, and Group B with small stimulation. Group A and Group B were performed with different stimulations. We found that, compared with saline control group, model group, and Group B, Group A showed that the skin temperature changes on right acupoint SP6 and SP10 increased significantly at 5 min–10 min interval. The skin temperature changes on left SP6 decreased at instant–5 min interval. The skin temperature changes on right SP10 decreased significantly at instant–5 min interval and 10 min–20 min interval. Thermogenic action along Spleen Meridian of Foot Greater Yin was manifested as simultaneous skin temperature increase on right SP6 and SP10 at 5 min–10 min interval after needling SP6, which was helpful to illustrate the relationship between the characteristic of Deqi and needle stimulations.

  7. Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1

    International Nuclear Information System (INIS)

    Ito, Tatsuo; Kitamura, Haruka; Uwatoko, Chisana; Azumano, Makiko; Itoh, Kohji; Kuwahara, Jun

    2010-01-01

    Research highlights: → Sp1 zinc fingers themselves interact with importin α. → Sp1 zinc finger domains play an essential role as a nuclear localization signal. → Sp1 can be transported into the nucleus in an importin-dependent manner. -- Abstract: Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood. In this study, we revealed that GST-fused Sp1 protein bound to endogenous importin α in HeLa cells via the Sp1 zinc finger domains, which comprise the DNA binding domain of Sp1. It was found that the Sp1 zinc finger domains directly interacted with a wide range of importin α including the armadillo (arm) repeat domain and the C-terminal acidic domain. Furthermore, it turned out that all three zinc fingers of Sp1 are essential for binding to importin α. Taken together, these results suggest that the Sp1 zinc finger domains play an essential role as a NLS and Sp1 can be transported into the nucleus in an importin-dependent manner even though it possesses no classical NLSs.

  8. Co-metabolism of DDT by the newly isolated bacterium, Pseudoxanthomonas sp. wax

    Directory of Open Access Journals (Sweden)

    Guangli Wang

    2010-06-01

    Full Text Available Microbial degradation of 1,1,1-trichloro-2,2-bis(p-chlorophenylethane (DDT is the most promising way to clean up DDT residues found in the environment. In this paper, a bacterium designated as wax, which was capable of co-metabolizing DDT with other carbon sources, was isolated from a long-term DDT-contaminated soil sample by an enrichment culture technique. The new isolate was identified as a member of the Pseudoxanthomonas sp., based on its morphological, physiological and biochemical properties, as well as by 16S rRNA gene analysis. In the presence of 100 mg l-1 glucose, the wax strain could degrade over 95% of the total DDT, at a concentration of 20 mg l-1, in 72 hours, and could degrade over 60% of the total DDT, at a concentration of 100 mg l-1, in 144 hours. The wax strain had the highest degradation efficiency among all of the documented DDT-degrading bacteria. The wax strain could efficiently degrade DDT at temperatures ranging from 20 to 37ºC, and with initial pH values ranging from 7 to 9. The bacterium could also simultaneously co-metabolize 1,1-dichloro-2,2-bis(p-chlorophenylethane (DDD, 2,2-bis(p-chlorophenyl-1,1-dichlorethylene (DDE, and other organochlorine compounds. The wax strain could also completely remove 20 mg kg-1 of DDT from both sterile and non-sterile soils in 20 days. This study demonstrates the significant potential use of Pseudoxanthomonas sp. wax for the bioremediation of DDT in the environment.

  9. Role of surfactant protein A (SP-A)/lipid interactions for SP-A functions in the lung.

    Science.gov (United States)

    Casals, C

    2001-01-01

    Surfactant protein A (SP-A), an oligomeric glycoprotein, is a member of a group of proteins named collectins that contain collagen-like and Ca(2+)-dependent carbohydrate recognition domains. SP-A interacts with a broad range of amphipathic lipids (glycerophospholipids, sphingophospholipids, glycosphingolipids, lipid A, and lipoglycans) that are present in surfactant or microbial membranes. This review summarizes SP-A/lipid interaction studies regarding the lipid system used (i.e., phospholipid vesicles, phospholipid monolayers, and lipids immobilized on silica or adsorbed on a solid support). The effect of calcium, ionic strength, and pH on the binding of SP-A to lipids and the subsequent lipid aggregation process is discussed. Current evidence suggests that hydrophobic-binding forces are involved in the peripherical association of SP-A to membranes. It is also proposed that fluid and liquid-ordered phase coexistence in surfactant membranes might favor partition of SP-A into those membranes. The binding of SP-A to surfactant membranes containing hydrophobic surfactant peptides makes possible the formation of a membrane reservoir in the alveolar fluid that is protected by SP-A against inactivation and improves the rate of surfactant film formation. In addition, the interaction of SP-A with membranes might enhance the affinity of SP-A for terminal carbohydrates of glycolipids or glycoproteins on the surface of invading microorganisms.

  10. SCExAO: First Results and On-Sky Performance

    Science.gov (United States)

    Currie, Thayne; Guyon, Olivier; Martinache, Frantz; Clergeon, Christophe; McElwain, Michael; Thalmann, Christian; Jovanovic, Nemanja; Singh, Garima; Kudo, Tomoyuki

    2013-01-01

    We present new on-sky results for the Subaru Coronagraphic Extreme Adaptive Optics imager (SCExAO) verifying and quantifying the contrast gain enabled by key components: the closed-loop coronagraphic low-order wavefront sensor (CLOWFS) and focal plane wavefront control ("speckle nulling"). SCExAO will soon be coupled with a high-order, Pyramid wavefront sensor which will yield greater than 90% Strehl ratio and enable 10(exp 6) -10(exp 7) contrast at small angular separations allowing us to image gas giant planets at solar system scales. Upcoming instruments like VAMPIRES, FIRST, and CHARIS will expand SCExAO's science capabilities.

  11. Staphylococcus aureus e Salmonella sp. em queijos de coalho artesanais produzidos em São Rafael, Rio Grande do Norte

    Directory of Open Access Journals (Sweden)

    Tarciara Magley da Fonseca Pereira

    2017-06-01

    Full Text Available O queijo de coalho artesanal é produto de origem animal muito consumido principalmente na região Nordeste do Brasil. É comumente fabricado com leite cru, não pasteurizado, como matéria prima, sem a utilização de boas práticas higiênicas, podendo veicular micro-organismos patogênicos ao consumidor. Assim, objetivou-se avaliar a presença de Salmonella sp. e quantificar Staphyloccocus aureus em queijos de coalho produzidos artesanalmente no município de São Rafael Rio Grande do Norte.Foram realizadas coletas em triplicata em sete queijarias artesanais do município, e, em seguida, os queijos foram transportados imediatamente em caixas isotérmicas, ao Laborátório de Biotecnologia Industrial da Universidade Federal Rural do Semi-Árido, onde foram submetidos à análise microbiológica de forma asséptica, conforme metodologia recomendada pela Instrução Normativa nº 62, de 26 de agosto de 2003, do Ministério da Agricultura, Pecuária e Abastecimento. Todas as amostras apresentaram elevadas contagens de Staphylococcus aureus, estando em desconformidade com o padrão exigido pela legislação brasileira para este grupo microbiano. Foi constatado ausência de Salmonella sp. nas amostras avaliadas. As análises microbiológicas evidenciaram péssimas condições de higiene de todos os queijos estudados, já que os mesmos apresentaram grande contaminação por Staphylococcus aureus, o que pode representar riscos à saúde pública.

  12. Five novel Wickerhamomyces- and Metschnikowia-related yeast species, Wickerhamomyces chaumierensis sp. nov., Candida pseudoflosculorum sp. nov., Candida danieliae sp. nov., Candida robnettiae sp. nov. and Candida eppingiae sp. nov., isolated from plants

    NARCIS (Netherlands)

    Groenewald, Marizeth; Robert, Vincent; Smith, Maudy Th

    On the basis of nucleotide divergences in the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacers (ITS) domain of the rRNA gene, five novel yeast species, Wickerhamomyces chaumierensis sp. nov. (CBS 8565(T)  = JCM 17246(T)), Candida pseudoflosculorum sp. nov. (CBS 8584(T)  = JCM

  13. Nematodes of amphibians from Java, Indonesia, with a description of new species, Meteterakis wonosoboensis n. sp. (Nematoda : Heterakoidea).

    Science.gov (United States)

    Purwaningsih, Endang; Dewi, Kartika; Hasegawa, Hideo

    2015-06-24

    During a survey on the parasites of amphibians of Indonesia, toads (30 Bufo melanostictus) and 246 frogs (213 Fejervarya cancrivora, 11 F. limnocharis, 22 Rana macrodon from West Java and 68 F. cancrivora from Central Java) were examined for parasitic nematodes. Three species of nematodes were found and described, i.e. Meteterakis wonosoboensis n. sp. from Fejervaria cancrivora; Meteterakis japonica from Bufo melanostictus, F. cancrivora and F. limnocharis; and Chabaudus sp. from F. cancrivora, F. limnocharis and Rana macrodon. Meteterakis wonosoboensis n. sp. is distinguished from other species of the genus by the length and shape of spicules, the number of caudal papillae, the presence of gubernaculum in male and the presence of vulval flap in female. Bufo melanostictus and Java are recorded as new host and locality for M. japonica, respectively.

  14. Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice

    Directory of Open Access Journals (Sweden)

    Mühlfeld Christian

    2007-10-01

    Full Text Available Abstract Background Surfactant protein D (SP-D deficient mice develop emphysema-like pathology associated with focal accumulations of foamy alveolar macrophages, an excess of surfactant phospholipids in the alveolar space and both hypertrophy and hyperplasia of alveolar type II cells. These findings are associated with a chronic inflammatory state. Treatment of SP-D deficient mice with a truncated recombinant fragment of human SP-D (rfhSP-D has been shown to decrease the lipidosis and alveolar macrophage accumulation as well as production of proinflammatory chemokines. The aim of this study was to investigate if rfhSP-D treatment reduces the structural abnormalities in parenchymal architecture and type II cells characteristic of SP-D deficiency. Methods SP-D knock-out mice, aged 3 weeks, 6 weeks and 9 weeks were treated with rfhSP-D for 9, 6 and 3 weeks, respectively. All mice were sacrificed at age 12 weeks and compared to both PBS treated SP-D deficient and wild-type groups. Lung structure was quantified by design-based stereology at the light and electron microscopic level. Emphasis was put on quantification of emphysema, type II cell changes and intracellular surfactant. Data were analysed with two sided non-parametric Mann-Whitney U-test. Main Results After 3 weeks of treatment, alveolar number was higher and mean alveolar size was smaller compared to saline-treated SP-D knock-out controls. There was no significant difference concerning these indices of pulmonary emphysema within rfhSP-D treated groups. Type II cell number and size were smaller as a consequence of treatment. The total volume of lamellar bodies per type II cell and per lung was smaller after 6 weeks of treatment. Conclusion Treatment of SP-D deficient mice with rfhSP-D leads to a reduction in the degree of emphysema and a correction of type II cell hyperplasia and hypertrophy. This supports the concept that rfhSP-D might become a therapeutic option in diseases that are

  15. [Investigation of Cryptosporidium sp. in workers of the Van municipality slaughterhouse and in slaughtered animals].

    Science.gov (United States)

    Ciçek, Mutalip; Körkoca, Hanifi; Gül, Abdurrahman

    2008-01-01

    This study was carried out in order to investigate the prevalence of Cryptosporidium sp. in slaughtered animals and workers of the Van municipality slaughterhouse in Van. Animals slaughtered at different times and workers who had been working in different departments of the slaughter house were included in the study for three months. A total of 309 fecal specimens from animals including 167 sheep, 56 goats and 86 cattle and 87 fecal specimens from workers were examined for Cryptosporidium sp. oocysts. In slaughtered animals, the modified acid-fast staining method was used to determine the oocysts of Cryptosporidium sp. The fecal samples of slaughter workers were examined by using RIDA (R) Quick Cryptosporidium Strip Test (R-Biopharm, Germany) and the modified acid-fast staining method. Fecal samples found to be positive by stripe test were also confirmed with the ELISA method (R-Biopharm, Germany). Oocysts of Cryptosporidium sp. were found in fecal specimens of 22 sheep (13.17%), 6 goats (10.71%) and 7 cattle (8.13%). Intestinal parasites were observed in 34 fecal specimens of workers (39.08%). Cryptosporidium sp., Hymenolepis nana, Chilomastix mesnili, Endolimax nana, Iodamoeba bütschlii were found in the specimen of one worker (1.14%), Entamoeba coli in 4 workers (4.59%), Blastocystis hominis (9.19%) in 8 workers, and Giardia intestinalis (19.54%) in 17 workers.

  16. Quality improvement on half-fin anchovy (Setipinna taty) fish sauce by Psychrobacter sp. SP-1 fermentation.

    Science.gov (United States)

    Zheng, Bin; Liu, Yu; He, Xiaoxia; Hu, Shiwei; Li, Shijie; Chen, Meiling; Jiang, Wei

    2017-10-01

    A method of improving fish sauce quality during fermentation was investigated. Psychrobacter sp. SP-1, a halophilic protease-producing bacterium, was isolated from fish sauce with flavor-enhancing properties and non-biogenic amine-producing activity. The performance of Psychrobacter sp. SP-1 in Setipinna taty fish sauce fermentation was investigated further. The inoculation of Psychrobacter sp. SP-1 did not significantly affect pH or NaCl concentration changes (P > 0.05), although it significantly increased total moderately halophilic microbial count, protease activity, total soluble nitrogen content and amino acid nitrogen content, and also promoted the umami taste and meaty aroma (P sauce quality by fermentation. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  17. Pentacyclic ingamine-type alkaloids, a new antiplasmodial pharmacophore from the marine sponge petrosid Ng5 Sp5

    Science.gov (United States)

    Two new pentacyclic ingamine- type alkaloids, namely 22(S)-hydroxyingamine A (2) and dihydroingenamine D (3), together with the known ingamine A (1) have been isolated from marine sponge Petrosid Ng5 Sp5 (Family: Petrosiidae) obtained from the open repository of National Cancer Institute, USA. The s...

  18. New strains of oil-degrading microorganisms for treating contaminated soils and wastes

    Science.gov (United States)

    Muratova, A. Yu; Panchenko, L. V.; Semina, D. V.; Golubev, S. N.; Turkovskaya, O. V.

    2018-01-01

    Two new strains Achromobacter marplatensis101n and Acinetobacter sp. S-33, capable of degrading 49 and 46% of oil within 7 days were isolated, identified, and characterized. The application of A. marplatensis 101n in combination with ammonium nitrate (100 mg·kg-1) for 30 days of cultivation resulted in the degradation of 49% of the initial total petroleum hydrocarbon content (274 g·kg-1) in the original highly acid (pH 4.9) oil-contaminated waste. Up to 30% of oil sludge added to a liquid mineral medium at a concentration of 15% was degraded after 10 days of cultivation of A. marplatensis 101n. Application of yellow alfalfa (Medicago falcata L.) plants with Acinetobacter sp. S-33 for bioremediation of oil-sludge-contaminated soil improved the quality of cleanup in comparison with the bacterium- or plant-only treatment. Inoculation of Acinetobacter sp. S-33 increased the growth of both roots and shoots by more than 40%, and positively influenced the soil microflora. We conclude that the new oil-degrading strains, Acinetobacter sp. S-33 and A. marplatensis 101n, can serve as the basis for new bioremediation agents for the treatment of oil contaminated soils and waste.

  19. Distribuição e Densidade de Nasutitermes sp. (Isoptera: Termitidae em Mata Ribeirinha do Rio Miranda, Pantanal Sul-Matogrossense, Brasil

    Directory of Open Access Journals (Sweden)

    Leandro Pereira Polatto

    2009-04-01

    Full Text Available Resumo. Este trabalho teve como objetivo analisar a influência da densidade arbórea e da borda sobre a quantidade e o volume nidal dos ninhos arborícolas de Nasutitermes sp., bem como, verificar a dependência do tamanho desses ninhos e a sua região de fixação em relação à arquitetura arbórea. A coleta de dados foi realizada em outubro de 2005, em um trecho da mata ribeirinha do rio Miranda no Pantanal Miranda-Abobral, sendo feita amostragem da quantidade e volume nidal dos ninhos arborícolas de Nasutitermes sp. e verificado a densidade de árvores de dossel através do método de amostragem por parcelas ao longo de um gradiente de profundidade na mata. Outros valores de arquitetura arbórea em que o ninho servia de abrigo também foram analisados, utilizando-se o coeficiente de correlação de Spearman, o teste de Kruskal-Wallis e o Qui-Quadrado. Dentre os resultados analisados, conclui-se que os ninhos arborícolas de Nasutitermes sp. não estão distribuídos ao acaso na mata. Mas sim, pode-se inferir que a quantidade e volume nidal, os locais de fixação, construção e desenvolvimento desses ninhos arborícolas na mata ribeirinha do rio Miranda sofrem interferência da estrutura arbórea da mata estudada. Nasutitermes sp. (Isoptera: Termitidae Distribution and Density in Riverine Forest of the River Miranda, Pantanal Sul-Matogrossense, Brazil.Abstract. This work had as objectives to analyze the influence of the arboreal density and of the border on the amount and the volumetric mass of the arboreal nests of Nasutitermes sp., and to verify the dependence of the size of those nests and your fixation area in relation to the arboreal architecture. In October of 2005, in a space of the riverine forest of the river Miranda in the Pantanal of the Miranda-Abobral was accomplished the collection of data, being made sampling of the amount and volumetric mass of the arboreal nests of Nasutitermes sp., and verified the density of dossal

  20. From spent graphite to amorphous sp2+sp3 carbon-coated sp2 graphite for high-performance lithium ion batteries

    Science.gov (United States)

    Ma, Zhen; Zhuang, Yuchan; Deng, Yaoming; Song, Xiaona; Zuo, Xiaoxi; Xiao, Xin; Nan, Junmin

    2018-02-01

    Today, with the massive application of lithium ion batteries (LIBs) in the portable devices and electric vehicles, to supply the active materials with high-performances and then to recycle their wastes are two core issues for the development of LIBs. In this paper, the spent graphite (SG) in LIBs is used as raw materials to fabricate two comparative high-capacity graphite anode materials. Based on a microsurgery-like physical reconstruction, the reconstructed graphite (RG) with a sp2+sp3 carbon surface is prepared through a microwave exfoliation and subsequent spray drying process. In contrast, the neural-network-like amorphous sp2+sp3 carbon-coated graphite (AC@G) is synthesized using a self-reconfigurable chemical reaction strategy. Compared with SG and commercial graphite (CG), both RG and AC@G have enhanced specific capacities, from 311.2 mAh g-1 and 360.7 mAh g-1 to 409.7 mAh g-1 and 420.0 mAh g-1, at 0.1C after 100 cycles. In addition, they exhibit comparable cycling stability, rate capability, and voltage plateau with CG. Because the synthesis of RG and AC@G represents two typical physical and chemical methods for the recycling of SG, these results on the sp2+sp3 carbon layer coating bulk graphite also reveal an approach for the preparation of high-performance graphite anode materials derived from SG.

  1. Halobacterium sp. SP1(1) as a starter culture for accelerating fish sauce fermentation.

    Science.gov (United States)

    Akolkar, A V; Durai, D; Desai, A J

    2010-07-01

    Application of Halobacterium sp. SP1(1) for the acceleration of fish sauce fermentation. Traditional fish sauce fermentation was mimicked using Halobacterium sp. SP1(1) as starter culture. Protease activity, peptide release and α-amino content (parameters used to monitor the progress of the fermentation) were high at day 10 in tests and day 20 in un-inoculated controls. The total protein and nitrogen contents were also high in tests compared with controls. The amino acid profile observed at the end of fermentation in experimental samples, when compared with the commercial sauce preparation, was found to be better with respect to flavour and aroma contributing amino acids as well as essential amino acid lysine. Microflora analysis of the final fish sauce revealed the absence of any nonhalophilic or halotolerant micro-organisms. The protease-producing halophilic isolates obtained from the fish sauce of eviscerated and uneviscerated controls were identified as Halobacterium sp. F1 and F2, respectively, by 16S rDNA sequence analysis. Exogenous augmentation of Halobacterium sp. SP1(1) accelerated the fish sauce fermentation process with an additive effect on the existing natural microflora present in the fish during fermentation. Halobacterium sp SP1(1), therefore, can be used as an important starter culture for accelerating the fish fermentation process, which is attributed to its extracellular protease. The present study is the first report on use of Halobacterium species as a starter culture for accelerating fish sauce fermentation. Use of halobacterial starter cultures may revolutionize the process in fish sauce industries by reducing the fermentation time and making the process more economical with improved nutritive value of product. Journal compilation © 2009 The Society for Applied Microbiology. No claim to Indian Government works.

  2. ExaSP2

    Energy Technology Data Exchange (ETDEWEB)

    2017-09-08

    ExaSP2 is a reference implementation of typical linear algebra algorithms and workloads for a quantum molecular dynamics (QMD) electronic structure code. The algorithm is based on a recursive second-order Fermi-Operator expansion method (SP2) and is tailored for density functional based tight-binding calculations of material systems.

  3. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource.

    Directory of Open Access Journals (Sweden)

    Akanksha Srivastava

    Full Text Available An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732 were isolated (paddy fields and ponds in the Banaras Hindu University, campus and five strains screened for anticancer potential using human colon adenocarcinoma (HT29 and human kidney adenocarcinoma (A498 cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer and MCF-10A (normal human epithelial exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress.

  4. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource.

    Science.gov (United States)

    Srivastava, Akanksha; Tiwari, Ratnakar; Srivastava, Vikas; Singh, Tej Bali; Asthana, Ravi Kumar

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for anticancer potential using human colon adenocarcinoma (HT29) and human kidney adenocarcinoma (A498) cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC) eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer) and MCF-10A (normal human epithelial) exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress.

  5. An epistatic effect of KRT25 on SP6 is involved in curly coat in horses.

    Science.gov (United States)

    Thomer, Annika; Gottschalk, Maren; Christmann, Anna; Naccache, Fanny; Jung, Klaus; Hewicker-Trautwein, Marion; Distl, Ottmar; Metzger, Julia

    2018-04-23

    Curly coat represents an extraordinary type of coat in horses, particularly seen in American Bashkir Curly Horses and Missouri Foxtrotters. In some horses with curly coat, a hypotrichosis of variable extent was observed, making the phenotype appear more complex. In our study, we aimed at investigating the genetic background of curly coat with and without hypotrichosis using high density bead chip genotype and next generation sequencing data. Genome-wide association analysis detected significant signals (p = 1.412 × 10 -05 -1.102 × 10 -08 ) on horse chromosome 11 at 22-35 Mb. In this significantly associated region, six missense variants were filtered out from whole-genome sequencing data of three curly coated horses of which two variants within KRT25 and SP6 could explain all hair phenotypes. Horses heterozygous or homozygous only for KRT25 variant showed curly coat and hypotrichosis, whereas horses with SP6 variant only, exhibited curly coat without hypotrichosis. Horses with mutant alleles in both variants developed curly hair and hypotrichosis. Thus, mutant KRT25 allele is masking SP6 allele effect, indicative for epistasis of KRT25 variant over SP6 variant. In summary, genetic variants in two different genes, KRT25 and SP6, are responsible for curly hair. All horses with KRT25 variant are additionally hypotrichotic due to the KRT25 epistatic effect on SP6.

  6. Caracterización de actinobacterias raras, degradadoras de lignocelulosa: demostración de actividad lacasa en dos aislados de Tsukamurella sp y Cellulosimicrobium sp

    Directory of Open Access Journals (Sweden)

    Enrique Luis Revollo Escudero

    2012-07-01

    Full Text Available Título en ingles: Characterization of lignocelluloses-degrading rare actinobacteria: Demostration of laccase activity in two isolates of Tsukamurella sp and Cellulosimicrobium sp Resumen: Las características fisicoquímicas de la lignina y su compactación con la celulosa han dificultado la explotación biotecnológica de enormes cantidades de biomasa vegetal. Las lacasas constituyen una subfamilia de oxidasas multicobre que intervienen en la despolimerización de la lignina. Si bien han sido ampliamente caracterizadas en los hongos, los estudios de la diversidad y las funcionalidades de las lacasas en los procariotas se han centrado especialmente en isoformas enzimáticas de Streptomyces sp. En este trabajo se aislaron 20 cepas de actinobacterias del suelo. La actividad lacasa de 17 de ellas fue evidenciada en ensayos cualitativos con guayacol y dos cepas seleccionadas fueron caracterizadas en detalle. Las pruebas morfológicas y el análisis de las secuencias del gen 16S rRNA apuntan a que estos dos aislados pertenecen a los géneros Tsukamurella y Cellulosimicrobium. En cultivo sumergido con agitación, AC01 (Tsukamurella sp. expresó una máxima actividad de oxidación de ABTS (2,2’-azino-bis-(3-etilbenzotiazolin-6-sulfonato de 108 U/L. Por otra parte, AC18 (Cellulosimicrobium sp. que había exhibido una actividad oxidativa de guayacol superior a las 16 cepas restantes y demostró ser resistente a niveles tóxicos de cobre, logró un valor máximo de oxidación del ABTS de 0,56 U/L. Estos resultados sugieren que en el aislado AC18 operaría un fenómeno de especificidad de sustrato o de inductor, regulador de la expresión y de la actividad lacasa cuantificable. La caracterización genómica y funcional de las lacasas de nuevas actinobacterias lignocelulósicas ampliará la gama de centros redox con aplicaciones biotecnológicas específicas, además de facilitar el establecimiento de sus relaciones evolutivas con las eucariotas

  7. Adesão ao tratamento no transtorno bipolar

    OpenAIRE

    Santin,Aida; Ceresér,Keila; Rosa,Adriane

    2005-01-01

    O transtorno bipolar (TB) é uma doença crônica, recorrente, presente em 1,5% da população, estando associada a altas taxas de mortalidade e prejuízos socioeconômicos. O lítio, a carbamazepina e o ácido valpróico são os estabilizadores de humor mais usados. Em tratamentos prolongados, como é o caso do TB, a má-adesão dos pacientes é uma das maiores dificuldades. Verifica-se que fatores ligados ao paciente, aos medicamentos e aos médicos possam ser responsáveis pela baixa adesão. A psicoeducaçã...

  8. In vitro rootting of Dioscoreas sp.

    Directory of Open Access Journals (Sweden)

    Irma Quintero

    2003-07-01

    Full Text Available The Universidad de Córdoba s Vegetal Tissue Culture Laboratory evaluated the effect of naphthalenacetic acid (NAA on in vitro rooting of three yam cultivars (Dioscorea sp. in 2000. The effect of four hormone levels (0, 0.3, 0.6 and 0.9 mg/1 was studied on three yam cultivars (Diamantes-22,003 and 005. A random experimental design was used employing 4x3 factorial arrangement and 20 repetitions; each experimental unit consisted of a glass receptacle containing the culture medium and the explant (one segment nodal. The variables considered were the number of roots and their thickness, culture medium oxidation and callus production. Findings showed that both the hormone and genotype had an effect on all those variables considered in the study and interaction was significant (P

  9. Elucidation of a Carotenoid Biosynthesis Gene Cluster Encoding a Novel Enzyme, 2,2′-β-Hydroxylase, from Brevundimonas sp. Strain SD212 and Combinatorial Biosynthesis of New or Rare Xanthophylls

    Science.gov (United States)

    Nishida, Yasuhiro; Adachi, Kyoko; Kasai, Hiroaki; Shizuri, Yoshikazu; Shindo, Kazutoshi; Sawabe, Akiyoshi; Komemushi, Sadao; Miki, Wataru; Misawa, Norihiko

    2005-01-01

    A carotenoid biosynthesis gene cluster mediating the production of 2-hydroxyastaxanthin was isolated from the marine bacterium Brevundimonas sp. strain SD212 by using a common crtI sequence as the probe DNA. A sequence analysis revealed this cluster to contain 12 open reading frames (ORFs), including the 7 known genes, crtW, crtY, crtI, crtB, crtE, idi, and crtZ. The individual ORFs were functionally analyzed by complementation studies using Escherichia coli that accumulated various carotenoid precursors due to the presence of other bacterial crt genes. In addition to functionally identifying the known crt genes, we found that one (ORF11, named crtG) coded for a novel enzyme, carotenoid 2,2′-β-hydroxylase, which showed intriguingly partial homology with animal sterol-C5-desaturase. When this crtG gene was introduced into E. coli accumulating zeaxanthin and canthaxanthin, the resulting transformants produced their 2-hydroxylated and 2,2′-dihydroxylated products which were structurally novel or rare xanthophylls, as determined by their nuclear magnetic resonance and high-performance liquid chromatography/photodiode array detector/atmospheric pressure chemical ionization mass spectrometry spectral data. The new carotenoid produced was suggested to have a strong inhibitory effect on lipid peroxidation. PMID:16085816

  10. Superhard sp{sup 2}–sp{sup 3} hybrid carbon allotropes with tunable electronic properties

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Meng; Ma, Mengdong; Zhao, Zhisheng; Yu, Dongli; He, Julong, E-mail: hjl@ysu.edu.cn [State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004 (China)

    2016-05-15

    Four sp{sup 2}–sp{sup 3} hybrid carbon allotropes are proposed on the basis of first principles calculations. These four carbon allotropes are energetically more favorable than graphite under suitable pressure conditions. They can be assembled from graphite through intralayer wrinkling and interlayer buckling, which is similar to the formation of diamond from graphite. For one of the sp{sup 2}–sp{sup 3} hybrid carbon allotropes, mC24, the electron diffraction patterns match these of i-carbon, which is synthesized from shock-compressed graphite (H. Hirai and K. Kondo, Science, 1991, 253, 772). The allotropes exhibit tunable electronic characteristics from metallic to semiconductive with band gaps comparable to those of silicon allotropes. They are all superhard materials with Vickers hardness values comparable to that of cubic BN. The sp{sup 2}–sp{sup 3} hybrid carbon allotroes are promising materials for photovoltaic electronic devices, and abrasive and grinding tools.

  11. Bacteriophage SP6 encodes a second tailspike protein that recognizes Salmonella enterica serogroups C2 and C3

    International Nuclear Information System (INIS)

    Gebhart, Dana; Williams, Steven R.; Scholl, Dean

    2017-01-01

    SP6 is a salmonella phage closely related to coliphage K1-5. K1-5 is notable in that it encodes two polysaccharide-degrading tailspike proteins, an endosialidase that allows it to infect E. coli K1, and a lyase that enables it to infect K5 strains. SP6 is similar to K1-5 except that it encodes a P22-like endorhamnosidase tailspike, gp46, allowing it to infect group B Salmonella. We show here that SP6 can also infect Salmonella serogroups C 2 and C 3 and that a mutation in a putative second tailspike, gp47, eliminates this specificity. Gene 47 was fused to the coding region of the N-terminal portion of the Pseudomonas aeruginosa R2 pyocin tail fiber and expressed in trans such that the fusion protein becomes incorporated into pyocin particles. These pyocins, termed AvR2-SP47, killed serogroups C 2 and C 3 Salmonella. We conclude that SP6 encodes two tail proteins providing it a broad host range among Salmonella enterica. - Highlights: • SP6 is a “dual specificity” bacteriophage that encodes two different receptor binding proteins giving it a broad host range. • These receptor binding proteins can be used to re-target the spectrum of R-type bacteriocins to Salmonella enterica. • Both SP6 and the engineered R-type bacteriocins can kill the Salmonella serovars most associated with human disease making them attractive for development as antimicrobial agents.

  12. O direito fundamental ao conteúdo do próprio trabalho: uma reconstrução normativa do direito ao trabalho

    Directory of Open Access Journals (Sweden)

    Leonardo Vieira Wandelli

    2016-12-01

    Full Text Available Resumo: Pretende-se enfrentar a inefetividade do direito humano e fundamental ao trabalho a partir de um esforço de reconstrução normativa da sua fundamentação, apoiada em um duplo pilar teórico, nas teorias das necessidades e nas teorias do reconhecimento. Com isso, não somente se recupera a importância do vínculo entre trabalho e dignidade humana, como se explicitam aspectos essenciais da centralidade do trabalho para os sujeitos. A partir disso, o direito ao trabalho se desvela não apenas como necessidade radical, tendente à transformação da institucionalidade vigente, mas também como direito positivado em um amplo leque de normas internacionais e nacionais, que contempla diversas dimensões normativas atualmente exigíveis. Propõe-se que, no centro desse direito, está um direito fundamental ao conteúdo do próprio trabalho, que determina juridicamente as condições da atividade e da organização do trabalho. Recente estudo adotado pelo Alto Comissariado em Direitos Humanos da Organização das Nações Unidas (ONU sobre o direito ao trabalho reforça essa compreensão. Palavras-chave: Direitos humanos. Direitos fundamentais. Direito ao trabalho. Psicodinâmica do trabalho. Direito do trabalho.

  13. Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers.

    Science.gov (United States)

    Camargo, O A; Souza, E A; Mendes-Costa, M C; Santos, J B; Soares, M A

    2007-09-30

    We examined the capacity of strains of Glomerella cingulata f. sp phaseoli fungus (Colletotrichum lindemuthianum sexual stage) to form recombinants, using random amplified polymorphic DNA (RAPD). Crosses of all possible combinations between strains 40, 42, 20, 21, 22, 23, 24, 25, and 26 were made on Petri dishes using M3 culture medium. The 42 x 21 cross produced the largest number of perithecia and five asci; the respective ascospores were isolated. RAPD analysis was performed on the parents and descendants. The 62 polymorphic RAPD bands obtained were used to assess the genetic similarity using the method of Sorence and Dice and clustering analysis in the form of a dendrogram by the UPGMA method. The RAPD markers allowed identification of recombinants from the cross between strains 42 and 21 of G. cingulata f. sp phaseoli and 40 ascospores presented 63 and 49% genetic similarity with parents 2 (strain 42) and 1 (strain 21), respectively.

  14. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    Energy Technology Data Exchange (ETDEWEB)

    Ilhan-Sungur, Esra, E-mail: esungur@istanbul.edu.tr [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Unsal-Istek, Tuba [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Cansever, Nurhan [Yıldız Technical University, Faculty of Chemistry-Metallurgy, Metallurgical and Materials Engineering Department, 34210 Esenler, Istanbul (Turkey)

    2015-07-15

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel.

  15. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    International Nuclear Information System (INIS)

    Ilhan-Sungur, Esra; Unsal-Istek, Tuba; Cansever, Nurhan

    2015-01-01

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel

  16. Analysis list: SP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SP2 Blood,Liver,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyus...hu-u/hg19/target/SP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/SP2.5.tsv http://dbarchive....biosciencedbc.jp/kyushu-u/hg19/target/SP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Blood.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Liver.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc

  17. The human luteinizing hormone receptor gene promoter: activation by Sp1 and Sp3 and inhibitory regulation.

    Science.gov (United States)

    Geng, Y; Tsai-Morris, C H; Zhang, Y; Dufau, M L

    1999-09-24

    To understand the transcriptional mechanism(s) of human LH receptor (LHR) gene expression, we have identified the dominant functional cis-elements that regulate the activity of the promoter domain (-1 to -176 bp from ATG). Mutagenesis demonstrated that the promoter activity was dependent on two Sp1 domains (-79 bp, -120 bp) in a transformed normal placental cell (PLC) and the choriocarcinoma JAR cell. Both elements interacted with endogenous Sp1 and Sp3 factors but not with Sp2 or Sp4. In Drosophila SL2 cells, the promoter was activated by either Sp1 or Sp3. An ERE half-site (EREhs) at -174 bp was inhibitory (by 100%), but was unresponsive to estradiol and did not bind the estrogen receptor or orphan receptors ERR1 and SF-1. The 5' upstream sequence (-177 to -2056 bp) inhibited promoter activity in PLC by 60%, but only minimally in JAR cells. Activation of the human LHR promoter through Sp1/3 factors is negatively regulated through EREhs and upstream sequences to exert control of gene expression. Copyright 1999 Academic Press.

  18. Rhodotorula bloemfonteinensis sp. nov., Rhodotorula eucalyptica sp. nov., Rhodotorula orientis sp. nov. and Rhodotorula pini sp. nov., yeasts isolated from monoterpene-rich environments.

    Science.gov (United States)

    Pohl, Carolina H; Smit, Martha S; Albertyn, Jacobus

    2011-09-01

    Recent rDNA sequencing of 25 isolates from a previous study, during which limonene-utilizing yeasts were isolated from monoterpene-rich environments by using 1,4-disubstituted cyclohexanes as sole carbon sources, led to the identification of four hitherto unknown Rhodotorula species. Analyses of the 26S rDNA D1/D2 region as well as the internal transcribed spacer (ITS) domain indicated that two isolates (CBS 8499(T) and CBS 10736) were identical and were closely related to Rhodotorula cycloclastica, a previously described limonene-utilizing yeast. These novel isolates differed from known yeast species and could be distinguished from R. cycloclastica by standard physiological tests. The other three isolates represent three novel Rhodotorula species, closely related to Sporobolomyces magnisporus. These three species could also be distinguished from other Rhodotorula species by standard physiological tests. Based on these results, we suggest that the new isolates represent novel species, for which the names Rhodotorula eucalyptica sp. nov. (type strain CBS 8499(T)  = NRRL Y-48408(T)), Rhodotorula pini sp. nov. (type strain CBS 10735(T)  = NRRL Y-48410(T)), Rhodotorula bloemfonteinensis sp. nov. (type strain CBS 8598(T)  = NRRL Y-48407(T)) and Rhodotorula orientis sp. nov. (type strain CBS 8594(T)  = NRRL Y-48719(T)) are proposed. R. eucalyptica and R. pini can also utilize limonene.

  19. [Isolation and purification of Mn-peroxidase from Azospirillum brasilense Sp245].

    Science.gov (United States)

    Kupriashina, M A; Selivanov, N Iu; Nikitina, V E

    2012-01-01

    Homogenous Mn-peroxidase of a 26-fold purity grade was isolated from a culture of Azospirillum brasilense Sp245 cultivated on a medium containing 0.1 mM pyrocatechol. The molecular weight of the enzyme is 43 kD as revealed by electrophoresis in SDS-PAAG. It was shown that the use of pyrocatechol and 2,2'-azino-bis(3-ethylbenzotiazoline-6-sulfonate) at concentrations of 0.1 and I mM as inductors increased the Mn-peroxidase activity by a factor of 3.

  20. Detection of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa)

    Science.gov (United States)

    Ekawati, ER; Yusmiati, S. N. H.

    2018-01-01

    Blood cockle (Anadara granosa) has high level of zinc and protein, which is beneficial for therapeutic function for malnourished particularly stunting case in children. Zinc in animal foods is more absorbable than that from vegetable food. Blood cockle (Anadara granosa) is rich in nutrient and an excellent environment for the growth of microorganisms. This research aimed to identify the contamination of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa). This was observation research with laboratory analysis. Salmonella sp. and Vibrio sp. were detected from blood cockle. Total plate count was determine of the total amount of the bacteria. Results detected from 20 samples of blood cockle showed that all samples were negative of Salmonella sp. and 1 sample positive Vibrio sp. The result of total plate count bacteria was < 5 x 105 colony/g sample.

  1. Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A.

    Science.gov (United States)

    Sever-Chroneos, Zvjezdana; Krupa, Agnieszka; Davis, Jeremy; Hasan, Misbah; Yang, Ching-Hui; Szeliga, Jacek; Herrmann, Mathias; Hussain, Muzafar; Geisbrecht, Brian V; Kobzik, Lester; Chroneos, Zissis C

    2011-02-11

    Staphylococcus aureus causes life-threatening pneumonia in hospitals and deadly superinfection during viral influenza. The current study investigated the role of surfactant protein A (SP-A) in opsonization and clearance of S. aureus. Previous studies showed that SP-A mediates phagocytosis via the SP-A receptor 210 (SP-R210). Here, we show that SP-R210 mediates binding and control of SP-A-opsonized S. aureus by macrophages. We determined that SP-A binds S. aureus through the extracellular adhesin Eap. Consequently, SP-A enhanced macrophage uptake of Eap-expressing (Eap(+)) but not Eap-deficient (Eap(-)) S. aureus. In a reciprocal fashion, SP-A failed to enhance uptake of Eap(+) S. aureus in peritoneal Raw264.7 macrophages with a dominant negative mutation (SP-R210(DN)) blocking surface expression of SP-R210. Accordingly, WT mice cleared infection with Eap(+) but succumbed to sublethal infection with Eap- S. aureus. However, SP-R210(DN) cells compensated by increasing non-opsonic phagocytosis of Eap(+) S. aureus via the scavenger receptor scavenger receptor class A (SR-A), while non-opsonic uptake of Eap(-) S. aureus was impaired. Macrophages express two isoforms: SP-R210(L) and SP-R210(S). The results show that WT alveolar macrophages are distinguished by expression of SP-R210(L), whereas SR-A(-/-) alveolar macrophages are deficient in SP-R210(L) expressing only SP-R210(S). Accordingly, SR-A(-/-) mice were highly susceptible to both Eap(+) and Eap(-) S. aureus. The lungs of susceptible mice generated abnormal inflammatory responses that were associated with impaired killing and persistence of S. aureus infection in the lung. In conclusion, alveolar macrophage SP-R210(L) mediates recognition and killing of SP-A-opsonized S. aureus in vivo, coordinating inflammatory responses and resolution of S. aureus pneumonia through interaction with SR-A.

  2. Cr(VI) reduction from contaminated soils by Aspergillus sp. N2 and Penicillium sp. N3 isolated from chromium deposits.

    Science.gov (United States)

    Fukuda, Tsubasa; Ishino, Yasuhiro; Ogawa, Akane; Tsutsumi, Kadzuyo; Morita, Hiroshi

    2008-10-01

    Aspergillus sp. N2 and Penicillium sp. N3 are chromate-resistant filamentous fungi that were isolated from Cr(VI) contaminated soil based on their ability to decrease hexavalent chromium levels in the growth medium. After 120 h of growth in a medium containing 50 ppm Cr(VI) at near neutral pH, Aspergillus sp. N2 reduced the Cr(VI) concentration by about 75%. Penicillium sp. N3 was able to reduce the Cr(VI) concentration by only 35%. However, Penicillium sp. N3 reduced the Cr(VI) concentration in the medium by 93% under acidic conditions. Interestingly, the presence of Cu(II) enhanced the Cr(VI) reducing ability of Aspergillus sp. N2 and Penicillium sp. N3 at near neutral pH. Aspergillus sp. N2 and Penicillium sp. N3 reduced the Cr(VI) concentration in the growth medium to a virtually undetectable level within 120 h. For both Aspergillus sp. N2 and Penicillium sp. N3, mycelial seed cultures were more efficient at Cr(VI) reduction than conidium seed cultures. The mechanisms of Cr(VI) reduction in Aspergillus sp. N2 and Penicillium sp. N3 were enzymatic reduction and sorption to mycelia. Enzymatic activity contributed significantly to Cr(VI) reduction. Aspergillus sp. N2 and Penicillium sp. N3 reduced the levels of Cr(VI) in polluted soil samples, suggesting that these strains might be useful for cleaning up chromium-contaminated sites.

  3. Detecção de proteínas imunorreativas de Rickettsia sp. cepa Mata Atlântica

    Directory of Open Access Journals (Sweden)

    Caroline S. Oliveira

    Full Text Available RESUMO: A Febre Maculosa Brasileira (FMB é uma doença infecciosa, transmitida por carrapatos ao homem. Uma nova riquetsiose humana foi descrita como causadora de Febre Maculosa no Estado de São Paulo, sendo denominada de Rickettsia sp. cepa Mata Atlântica. O presente trabalho teve como objetivo detectar e identificar proteínas com potencial de estimular o sistema imune de hospedeiro mamífero, desta nova cepa descrita. Para tanto, foi realizado a extração proteica total de Rickettsia sp. cepa Mata Atlântica. As proteínas extraídas foram fracionadas por eletroforese. As bandas proteicas foram transferidas para membranas de nitrocelulose por migração elétrica e submetidas à técnica de Western-blot, para detecção proteica. Ao todo sete proteínas imunorreativas foram detectadas. Duas proteínas apresentaram maior abundancia, com peso molecular, de 200 e 130 kDa respectivamente. Através da comparação de mapas proteômicos existentes e pelo peso molecular que estas proteínas apresentaram, sugere-se que as duas proteínas detectadas representem rOmpA (200 kDa e rOmpB (130 kDa. As demais proteínas detectadas apresentaram menor ocorrência e peso molecular inferior a 78 kDa, podendo representar membros da família de antígenos de superfície celular (Sca - Surface cell antigen. As proteínas detectadas poderão servir como base de estudo na elaboração de métodos diagnósticos sensíveis e específicos, no desenvolvimento de vacinas, além de possibilitarem novos estudos para terapias mais eficazes.

  4. Penggunaan Jamur Antagonis Trichoderma SP. Dan Gliocladium SP. Untuk Mengendalikan Penyakit Layu Fusarium Pada Tanaman Bawang Merah (Allium Ascalonicum L.)

    OpenAIRE

    Arie Ramadhina, Arie Ramadhina; Lisnawita, Lisnawita; Lubis, Lahmuddin

    2013-01-01

    The use of antagonism fungus of Trichoderma sp. and Gliocladium sp. for controlling wilt(Fusarium oxysporum) in red onion plants. The aim of the research was to know the effectiviness ofantagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants.The research used non-factorial RAK (random group design) with eight treatments: control, 10grams of F. oxysporum, 12 grams of Trichoderma sp., 18 grams of Trichoderma sp., 24 grams ofTrichoderma sp., and 12 grams ...

  5. In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.Efeito in vitro do óleo essencial de Eucalyptus globulus sobre o crescimento e desenvolvimento de Penicillium sp.

    Directory of Open Access Journals (Sweden)

    Marcia Holanda Nozaki

    2011-08-01

    Full Text Available Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar, negative control (PDA with addition of 40mg of azoxystrobin/L and positive control (PDA media only. The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA.O fungo Penicillium sp. é o agente causal dos bolores, considerada a principal doença pós-colheita em citros, levando à perdas na qualidade e quantidade dos frutos comercializáveis. Além de perdas econômicas, uma vez que nas perdas pós-colheitas estão inclusos os custos, transporte e armazenagem de frutos. O presente trabalho teve por objetivo verificar a ação fungitóxica do óleo essencial de Eucalyptus globulus no controle in vitro de Penicillium sp., avaliando seu crescimento micelial, produção e a germinação de esporos. O experimento foi conduzido no

  6. NCBI nr-aa BLAST: CBRC-TNIG-22-0071 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0071 ref|NP_001832.1| cannabinoid receptor 2 (macrophage) [Homo sapien...s] sp|P34972|CNR2_HUMAN Cannabinoid receptor 2 (CB2) (CB-2) (CX5) emb|CAA52376.1| CB2 (peripheral) cannabino...id receptor [Homo sapiens] emb|CAD22548.1| peripheral cannabinoid receptor CB2 [Homo sapiens] emb|CAD22549.1| peripheral cann...abinoid receptor CB2 [Homo sapiens] gb|AAO92299.1| cannabinoid r...eceptor 2 [Homo sapiens] emb|CAI14799.1| cannabinoid receptor 2 (macrophage) [Homo sapiens] emb|CAJ42137.1| cann

  7. NCBI nr-aa BLAST: CBRC-TNIG-22-0079 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0079 ref|NP_000862.1| 5-hydroxytryptamine (serotonin) receptor 6 [Homo... sapiens] sp|P50406|5HT6R_HUMAN 5-hydroxytryptamine 6 receptor (5-HT-6) (Serotonin receptor 6) gb|AAA92622.1| 5-HT6 serotonin... receptor gb|AAR07900.1| 5-hydroxytryptamine/serotonin receptor 6 [Homo sapiens] gb|AAH7499...6.1| 5-hydroxytryptamine (serotonin) receptor 6 [Homo sapiens] gb|AAH74995.1| 5-hydroxytryptamine (seroton...in) receptor 6 [Homo sapiens] emb|CAI19020.1| 5-hydroxytryptamine (serotonin) recep

  8. Florescimento e produção de maracujazeiro-amarelo obtido por enxertia hipocotiledonar em Jaboticabal-SP e Araguari-MG

    Directory of Open Access Journals (Sweden)

    Geraldo Costa Nogueira Filho

    2011-03-01

    Full Text Available O trabalho foi realizado no ripado e na área de Fruticultura do Departamento de Produção Vegetal da Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP, em Jaboticabal-SP, e em área de pequeno produtor fornecedor da Maguari (Kraft Foods do Brasil em Araguari-MG, tendo por objetivo avaliar o desenvolvimento de maracujazeiro-amarelo enxertado por enxertia hipocotiledonar, sobre seis espécies de Passifloraceas. Foram utilizados sete tratamentos, sendo seis tratamentos com as espécies: P. edulis f. flavicarpa, P. caerulea, P. alata, P. gibertii, P. coccinea, P. cincinnata e um tratamento com pé-franco de P. edulis f. flavicarpa. Para todos os tratamentos, a variedade-copa utilizada foi o maracujazeiro-amarelo 'FB 200'. O delineamento experimental utilizado foi o de blocos ao acaso, com três repetições. A parcela foi constituída de uma linha de quatro plantas (20m lineares. A condução foi realizada conforme os tratos culturais recomendados para a cultura. As características avaliadas foram florescimento, produção, número e peso médio de frutos. Em Jaboticabal-SP, o pé-franco apresentou melhor desenvolvimento e maior produção que as plantas enxertadas. Em Araguari-MG, P. edulis f. flavicarpa, P. caerulea e pé-franco apresentaram melhor produção. Não houve diferença significativa no número de frutos, e o P. alata diminuiu, em relação aos outros porta-enxertos, o peso dos frutos.

  9. Neuroprotective Compound from an Endophytic Fungus, Colletotrichum sp. JS-0367.

    Science.gov (United States)

    Song, Ji Hoon; Lee, Changyeol; Lee, Dahae; Kim, Soonok; Bang, Sunghee; Shin, Myoung-Sook; Lee, Jun; Kang, Ki Sung; Shim, Sang Hee

    2018-05-23

    Colletotrichum sp. JS-0367 was isolated from Morus alba (mulberry), identified, and cultured on a large scale for chemical investigation. One new anthraquinone (1) and three known anthraquinones (2-4) were isolated and identified using spectroscopic methods including 1D/2D-NMR and HRESIMS. Although the neuroprotective effects of some anthraquinones have been reported, the biological activities of the four anthraquinones isolated in this study have not been reported. Therefore, the neuroprotective effects of these compounds were determined against murine hippocampal HT22 cell death induced by glutamate. Compound 4, evariquinone, showed strong protective effects against HT22 cell death induced by glutamate by the inhibition of intracellular ROS accumulation and Ca 2+ influx triggered by glutamate. Immunoblot analysis revealed that compound 4 reduced the phosphorylation of MAPKs (JNK, ERK1/2, and p38) induced by glutamate. Furthermore, compound 4 strongly attenuated glutamate-mediated apoptotic cell death.

  10. Penggunaan Streptomyces sp. Sebagai Biokontrol Penyakit Layu Pada Tanaman Cabai Merah (Capsicum annuum L. yang Disebabkan Oleh Fusarium oxysporum f.sp. capsici

    Directory of Open Access Journals (Sweden)

    ANINDA OKTAVIA RAHARINI

    2014-01-01

    Full Text Available A research has been conducted to find out Streptomyces bacteria at Bukit Jimbaran, to inhibitionpotency of Streptomyces sp. to pathogenic fungi Fusarium oxysporum f.sp. capsici, and to find outantifungal activity of Streptomyces filtrate to F.oxysporum f.sp. capsici in chili (Capsicum annuumL. plants. Streptomyces sp. isolation was done by platting method with selective media YMA (ISP4.Identification of Streptomyces sp. used Bergey’s book entitled Manual Determinative Bacteriology.Test inhibition against F.oxysporum f.sp. capsici and in vivo test used by dying the roots of the chili(C.annuum L. plant with F.oxysporum f.sp. capsici and after 30 seconds the roots were dying withStreptomyces sp. culture, furthermore sterile soil on polybag watered by F.oxysporum f.sp. capsicispore and Streptomyces sp. culture at the same time. The result found five isolates Streptomyces sp.with different morphological. The antagonis test showed Streptomyces sp. 4 had ability (82% againstFusarium, Streptomyces sp.1 (72%, Streptomyces sp.2 (64%, Streptomyces sp.3 (76%, andStreptomyces sp. 5 (32%. All Streptomyces suppressed the growth of Fusarium on chili plants inglass house (p<0,05. Streptomyces sp.4 suppressed Fusarium wilt disease in chili from 80% in controlto 8%.

  11. Suporte relacionado ao cuidado em saúde ao doente com aids: o modelo de comboio e a enfermagem

    Directory of Open Access Journals (Sweden)

    Edilene Aparecida Araújo da Silveira

    2011-06-01

    Full Text Available O presente estudo teve como objetivo conhecer a relação e a troca de suporte entre o doente com AIDS e pessoas à sua volta. O estudo está baseado no modelo de comboio das relações sociais que tem como uma de suas características a representação da rede em três círculos concêntricos. Os participantes eram pessoas que estavam internadas e que falaram sobre o suporte relacionado ao cuidado em saúde proporcionado por pessoas próximas, ou seja, que pertenciam ao círculo interno do comboio. A maioria dos participantes descreveu o círculo como sendo composto por no máximo cinco integrantes, sendo que pessoas da família foram as mais citadas. Os profissionais de saúde precisam conhecer o paciente e seu comboio, reconhecendo-os no contexto psicossocial e cultural de forma a favorecer a aceitação da soropositividade, mudanças no estilo de vida, ajuda nos cuidados de saúde e adesão ao tratamento.

  12. Obesidade e fatores de risco cardiovascular em estudantes de Sorocaba, SP Obesity and cardiovascular risk factors in school children from Sorocaba, SP

    Directory of Open Access Journals (Sweden)

    Isabela Annunziato Ramos Mazaro

    2011-12-01

    Full Text Available OBJETIVO: Verificar a prevalência de obesidade, hipertensão arterial sistêmica (HAS, aumento de cintura e acanthosis nigricans em estudantes da cidade paulistana de Sorocaba em 2009, associando aos fatores de risco. MÉTODOS: Amostra probabilística com 680 crianças (7-11 anos de 13 escolas municipais de Sorocaba. Um questionário avaliou a prática de atividade física, tempo com televisão, videogame e computador (TV/VG/PC, antecedentes do aluno e dos pais para hipertensão arterial, doença renal e cardíaca e nível econômico. Foram realizadas medidas de peso, altura, cintura, pressão arterial (PA e verificado acanthosis nigricans. Determinou-se a prevalência de alteração nutricional, HAS, aumento da cintura e acanthosis nigricans. Para a associação do índice de massa corpórea (IMC IMC > P85 e da PA > P90 com outras variáveis, empregou-se o teste do qui-quadrado ou exato de Fisher (p P85 foi 22,1% [IC 95%: 19,0-25,3%], de PA > P90 10,9% [IC 95%: 8,6-13,5%], aumento da cintura 15,4% [IC 95%: 12,9-17,9%] e acanthosis nigricans 3,8% [IC 95%: 2,6-5,6%]. Foi encontrada associação do excesso de peso com antecedentes do pai (RCP: 1,76; IC 95%:1,05-2,95; p = 0,02 em ambas as análises. A elevação de PA associou-se ao sexo feminino (RCP: 1,90; IC 95%:1,12-3,23; p = 0,010, ao maior tempo na TV/VG/PC (RCP: 1,82; IC 95%:1,00-3,36; p = 0,030, à presença de acanthosis nigricans (RCP: 8,18; IC 95%:3,37-19,80; p OBJECTIVE: To verify the prevalence of obesity, systemic arterial hypertension (SAH, waist circumference and acanthosis nigricans (AN in school children from Sorocaba, in 2009 and associate them with risk factors. METHODS: A probabilistic sample study was carried out with 680 children (7-11 years from 13 public schools from the city of Sorocaba, SP. A questionnaire containing questions on physical activity, time spent watching television, playing with videogames and computers (TV/VG/PC, student and parental antecedents of

  13. Guide til gode spørgeskemaer

    DEFF Research Database (Denmark)

    Olsen, Henning

    Spørgeskemaundersøgelser bliver ofte brugt til at dokumentere forskellige forhold og begrunde politiske beslutninger. Men resultaterne kan være forbundet med stor usikkerhed. Det kræver omhu og stor sproglig formåen at udarbejde spørgeskemaer. Seniorforsker Henning Olsen har i flere år arbejdet med...... viden om, hvordan folk forstår sproglige meddelelser og genkalder sig informationer. I guiden behandles emner som fx styrende problemstillinger og spørgsmåls fokus og neutralitet, formulering af åbne eller lukkede spørgsmål og svarkategorier, tematiske spørgeforløb, aflastning af svarpersoners...

  14. DISTRIBUSI Solen sp DI PERAIRAN KABUPATEN BANGKALAN

    Directory of Open Access Journals (Sweden)

    Eva Ari Wahyuni

    2016-03-01

    Full Text Available DISTRIBUTION OF Solen sp IN BANGKALAN WATERSSolen sp potential needs to be developed on the island of Madura, particularly in Bangkalan. Solen sp utilization has increased which has the potential to overfishing. Therefore, this study aims to determine the density of Solen sp and their ecology in the waters Modung village, Modung District, Bangkalan. The experiment was conducted in April 2015 using the descriptive method. The materials used include Solen sp and physico-chemical parameters of the environment (temperature, salinity, pH, and substrate. The analyzes were conducted at the Laboratory of Marine Science, Department of Marine Sciences, Trunojoyo University of Madura by using the tool grabsampler, sieveshaker, and pipetting with gravimetric method. The analysis shows the range of values of temperature between 29-300C, salinity between 31-32 ppt, pH were 7.9-8.0 and the type of substrate in the form of sandy mud, as well as the density of Solen sp from 8-10 individuals/m2. All measurement results indicate normal conditions and in accordance with the sea water quality standard for marine life, which can be a suitable habitat for the growth and development of Solen sp. This condition is thought to affect the density of Solen sp.Keywords: Bangkalan, density, distribution, Solen sp, substrate.ABSTRAKPotensi Solen sp perlu dikembangkan di pulau Madura, khususnya di Kabupaten Bangkalan. Pemanfaatan Solen sp mengalami peningkatan sehingga berpotensi overfishing. Untuk itu, penelitian ini bertujuan untuk mengetahui kepadatan Solen sp dan ekologinya di perairan desa Modung, Kecamatan Modung, Kabupaten Bangkalan. Penelitian dilaksanakan pada bulan April 2015 dengan metode deskriptif. Materi dan bahan yang digunakan diantaranya Solen sp dan parameter fisika-kimia lingkungan (suhu, salinitas, pH, dan substrat. Analisa dilakukan di Laboratorium Ilmu Kelautan, Program studi/Jurusan Ilmu Kelautan Universitas Trunojoyo Madura dengan menggunakan alat

  15. TRABALHADORES DO MAR: UMA DISCUSSÃO SOBRE AS TRANSFORMAÇÕES DO TRABALHO DO PESCADOR ARTESANAL DE UBATUBA/SP

    Directory of Open Access Journals (Sweden)

    Larissa Tavares Moreno

    2013-07-01

    Full Text Available Este artigo realiza uma discussão quanto ao trabalho do pescador artesanal, mais propriamente, visando apreender as transformações do trabalho nesta atividade, assim como, suas particularidades, limites e entraves enfrentados por estes sujeitos que vivem do trabalho da pesca artesanal no município de Ubatuba/SP. Neste sentido, revelando a importância de estudos geográficos nesta área, comprometidos com esses trabalhadores artesanais do mar, revelando como o seu trabalho (e modo de vida atualmente são ainda muito atuantes, mesmo que marcados pelas processualidades conflitantes do capital, que levam a sua desorganização.

  16. Representações sociais e adesão ao tratamento antirretroviral (Social Representations and Adherence to Antiretroviral Treatment

    Directory of Open Access Journals (Sweden)

    Brigido Vizeu Camargo

    2014-12-01

    Full Text Available Resumo Este trabalho teve como objetivo identificar as representações sociais e os fatores que influenciam a adesão ao tratamento antirretroviral. Participaram do estudo 154 pessoas que faziam tratamento antirretroviral, sendo 82 homens com média de idade de 40 anos e 3 meses. Os dados foram obtidos por meio de entrevistas diretivas e por uma escala de adesão ao tratamento antirretroviral. O material textual sobre o significado do tratamento foi analisado pelo software IRAMUTEQ. O tratamento é representado a partir de duas concepções: a primeira a uma vida normal mantida pela ideia de uso contínuo e regular dos medicamentos, compartilhada pelas pessoas com alta adesão ao tratamento. Já a segunda, como algo que gera sofrimento e isolamento social, mais característica de pessoas com baixa/inadequada e insuficiente/ regular adesão. Sobre o nível de adesão, os resultados indicaram que 22.7% dos participantes apresentaram adesão alta/ estrita, e que a relação com o médico, a equipe de saúde e a informação sobre o tratamento estão relacionados com a alta/estrita adesão ao tratamento antirretroviral dos participantes. Abstract This study aims to identify the social representations and the factors that influence in the adherence to antiretroviral treatment. The participants in the study were 154 people who were undergoing antiretroviral treatment, 82 men with an average age of 40 years and three months. The data was obtained through direct interviews and through a scale of adherence to antiretroviral treatment. The textual material in relation to the meaning of the treatment was analyzed by the IRAMUTEQ software. The treatment is represented in two concepts: First, from a normal life maintained by the idea of continuous and regular use of medications shared by people with high adherence to treatment. Second, as something which generates social suffering, and isolation, most characteristic of people with low/inadequate, and

  17. Food and feeding habits of Omobranchus sp. (Blenniidae: Omobranchini) larvae in the Seagrass-Mangrove ecosystem of Johor Strait, Malaysia.

    Science.gov (United States)

    Ara, Roushon; Arshad, Aziz; Amin, S M Nurul; Ghaffar, Mazlan Abd

    2016-07-01

    The stomach contents of Omobranchus sp. (family Blenniidae) larvae were investigated in a seagrass-mangrove based ecosystem in Johor Strait, Malaysia from October 2007 to September 2008. Specimens of larval fish were collected through subsurface towing of a Bongo net from five different stations. The stomach sacs of 267 Omobranchus sp. larvae were separated and observed, which comprised of 24 significant food stuffs belonging to 6 main groups viz. phytoplankton (62.45%), zooplankton (18.24%), algae (5.56%), plant-like particles (5.75%), debris (4.22%) and unidentified particles (2.03%). In situ water parameters were also measured throughout the sampling cruises. There was a strong and significant positive correlation between stomach phytoplankton and salinity (r = 0.658, p < 0.05).? Canonical correlation analysis indicated a weak relationship (29.8%) between stomach contents and physico-chemical parameters. Only salinity appeared to be the controlling factor for the stomach contents of Omobranchus sp. larvae in the investigated area. Based on the stomach content analysis, it could be concluded that Omobranchus sp. were mainly herbivorous during the larval stages. ?

  18. Enhanced biodegradation of methylhydrazine and hydrazine contaminated NASA wastewater in fixed-film bioreactor.

    Science.gov (United States)

    Nwankwoala, A U; Egiebor, N O; Nyavor, K

    2001-01-01

    The aerobic biodegradation of National Aeronautics and Space Administration (NASA) wastewater that contains mixtures of highly concentrated methylhydrazine/hydrazine, citric acid and their reaction product was studied on a laboratory-scale fixed film trickle-bed reactor. The degrading organisms, Achromobacter sp., Rhodococcus B30 and Rhodococcus J10, were immobilized on coarse sand grains used as support-media in the columns. Under continuous flow operation, Rhodococcus sp. degraded the methylhydrazine content of the wastewater from a concentration of 10 to 2.5 mg/mL within 12 days and the hydrazine from approximately 0.8 to 0.1 mg/mL in 7 days. The Achromobacter sp. was equally efficient in degrading the organics present in the wastewater, reducing the concentration of the methylhydrazine from 10 to approximately 5 mg/mL within 12 days and that of the hydrazine from approximately 0.8 to 0.2 mg/mL in 7 days. The pseudo first-order rate constants of 0.137 day(-1) and 0.232 day(-1) were obtained for the removal of methylhydrazine and hydrazine, respectively, in wastewater in the reactor column. In the batch cultures, rate constants for the degradation were 0.046 and 0.079 day(-1) for methylhydrazine and hydrazine respectively. These results demonstrate that the continuous flow bioreactor afford greater degradation efficiencies than those obtained when the wastewater was incubated with the microbes in growth-limited batch experiments. They also show that wastewater containing hydrazine is more amenable to microbial degradation than one that is predominant in methylhydrazine, in spite of the longer lag period observed for hydrazine containing wastewater. The influence of substrate concentration and recycle rate on the degradation efficiency is reported. The major advantages of the trickle-bed reactor over the batch system include very high substrate volumetric rate of turnover, higher rates of degradation and tolerance of the 100% concentrated NASA wastewater. The

  19. SP-A binding sites on bovine alveolar macrophages.

    Science.gov (United States)

    Plaga, S; Plattner, H; Schlepper-Schaefer, J

    1998-11-25

    Surfactant protein A (SP-A) binding to bovine alveolar macrophages was examined in order to characterize SP-A binding proteins on the cell surface and to isolate putative receptors from these cells that could be obtained in large amounts. Human SP-A, unlabeled or labeled with gold particles, was bound to freshly isolated macrophages and analyzed with ELISA or the transmission electron microscope. Binding of SP-A was inhibited by Ca2+ chelation, by an excess of unlabeled SP-A, or by the presence of 20 mg/ml mannan. We conclude that bovine alveolar macrophages expose binding sites for SP-A that are specific and that depend on Ca2+ and on mannose residues. For isolation of SP-A receptors with homologous SP-A as ligand we isolated SP-A from bovine lung lavage. SDS-PAGE analysis of the purified SP-A showed a protein of 32-36 kDa. Functional integrity of the protein was demonstrated. Bovine SP-A bound to Dynabeads was used to isolate SP-A binding proteins. From the fractionated and blotted proteins of the receptor preparation two proteins bound SP-A in a Ca2+-dependent manner, a 40-kDa protein showing mannose dependency and a 210-kDa protein, showing no mannose sensitivity. Copyright 1998 Academic Press.

  20. Protease of Stenotrophomonas sp. from Indonesian fermented food: gene cloning and analysis

    Directory of Open Access Journals (Sweden)

    Frans Kurnia

    2018-02-01

    Full Text Available Screening of proteolytic and fibrinolytic bacteria from Indonesian soy bean based fermented food Oncom revealed several potential isolates. Based on 16s rDNA gene analysis, one particular isolate with the highest proteolytic and fibrinolytic activity was identified as Stenotrophomonas sp. The protease gene was amplified to generate a 1749 bp Polymerase Chain Reaction product and BLAST analysis, revealed 90% homology with gene encoding protease enzyme from Stenotrophomonas maltophilia. The putative amino acid sequence indicated a serine protease enzyme with typical amino acid aspartate, histidine and serine in the catalytic triad. The gene was translated into a pre-pro-protein consisted of cleavage site on its N terminal and Pre-Peptidase Cterminal domain. Cloning of the protease gene in pET22b with Escherichia coli BL21 DE3 as the host showed that the gene was expressed as insoluble protein fraction. This is the first report for analysis of protease gene from food origin Stenotrophomonas sp.

  1. Heparinoides naturais isolados de rodofíceas (Halymenia sp. arribadas na costa cearense - doi: 10.4025/actascibiolsci.v32i3.6282 Natural heparinoids isolated from Halymenia sp. (Rhodophyceaes delivery on the Ceará coast - doi: 10.4025/actascibiolsci.v32i3.6282

    Directory of Open Access Journals (Sweden)

    Wladimir Ronald Lobo Farias

    2010-09-01

    Full Text Available A crescente carência de heparina (HEP motiva a busca por fontes alternativas de novos anticoagulantes naturais. Objetivou-se avaliar a atividade anticoagulante dos polissacarídeos sulfatados (PS isolados de uma rodofícea do gênero Halymenia, nativa do litoral cearense, Brasil. Os PS totais foram obtidos por digestões consecutivas com papaína em tampão acetato de sódio 0,1 M (pH 5,0, contendo cisteína 5 mM e EDTA 5 mM, seguidas por cromatografia de troca iônica em coluna de DEAE-celulose. As frações obtidas foram concentradas por liofilização e submetidas à eletroforese em gel de agarose a 0,5%. Os ensaios anticoagulantes foram realizados pelo tempo de tromboplastina parcial ativada (TTPA, usando-se plasma de coelho e uma curva padrão de HEP (100 UI MG-1. As extrações (53,96% mostraram diferenças marcantes durante o fracionamento e no grau de resolução dos PS. A espécie apresentou PS com atividade anticoagulante superior a HEP. O TTPA das frações modificou-se acentuadamente entre as extrações, expressando-se de maneira dose-dependente e sofrendo um acréscimo de 110,40 (1ª extração para 143,10 UI MG-1 (3ª extração. Os resultados sugerem que a atividade anticoagulante dos PS isolados de Halymenia sp. foi promovida pela inibição da via intrínseca e/ou comum da cascata de coagulação. As modificações no TTPA possivelmente serão elucidadas pelos mecanismos de ação envolvidos na coagulação e caracterização estrutural desses compostos. Portanto, a rodofícea Halymenia sp. é uma boa fonte de heparinoides e sugerem-se estudos relacionados ao cultivo da espécie, em proteção aos bancos de algas.The increasing demand for heparin (HEP has led to a search for alternative sources of natural anticoagulants. This study aimed to evaluate the anticoagulant activity of sulfated polysaccharides (SP isolated from a Halymenia rhodophyceae genus native to the coast of Ceará, Brazil. Total SP were obtained by consecutive

  2. Remote sensing data of SP Mountain and SP Lava flow in North-Central Arizona

    Science.gov (United States)

    Schaber, G.G.; Elachi, C.; Farr, T.G.

    1980-01-01

    Multifrequency airborne radar image data of SP Mountain [Official name of feature (U.S. Geological Survey, 1970)] and SP flow (and vicinity) in north-central Arizona were obtained in diverse viewing directions and direct and cross-polarization, then compared with surface and aerial photography, LANDSAT multispectral scanner data, airborne thermal infrared imagery, surface geology, and surface roughness statistics. The extremely blocky, basaltic andesite of SP flow is significantly brighter on direct-polarization K-band (0.9-cm wavelength) images than on cross-polarized images taken simultaneously. Conversely, for the longer wavelength (25 cm) L-band radar images, the cross-polarization image returns from SP flow are brighter than the direct-polarized image. This effect is explained by multiple scattering and the strong wavelength dependence of polarization effects caused by the rectilinear basaltic andesite scatters. Two distinct types of surface relief on SP flow, one extremely blocky, the other subdued, are found to be clearly discriminated on the visible and thermal wavelength images but are separable only on the longer wavelength L-band radar image data. The inability of the K- and X- (3-cm wavelength) band radars to portray the differences in roughness between the two SP flow surface units is attributed to the radar frequency dependence of the surface-relief scale, which, described as the Rayleigh criterion, represents the transition between quasispecular and primarily diffuse backscatter. ?? 1980.

  3. Defense Acquisition Research Journal. Volume 22, Number 4, Issue 75, October 2015

    Science.gov (United States)

    2016-04-20

    operational effective- ness and operational suitability analyses, the AoA will provide thresholds for certain per forma nce pa ra meters ba sed on...project. 400 Defense ARJ, October 2015, Vol. 22 No. 4 : 394–415 A Publication of the Defense Acquisition University http://www.dau.mil Additionally...Resnick et al., 2011), and when a story is added to a sprint the acceptance criteria can be adjusted. This is where the customer communi- cates system

  4. Comparison of the Complior Analyse device with Sphygmocor and Complior SP for pulse wave velocity and central pressure assessment.

    Science.gov (United States)

    Stea, Francesco; Bozec, Erwan; Millasseau, Sandrine; Khettab, Hakim; Boutouyrie, Pierre; Laurent, Stéphane

    2014-04-01

    The Complior device (Alam Medical, France) was used in epidemiological studies which established pulse wave velocity (PWV) as a cardiovascular risk marker. Central pressure is related, but complementary to PWV and also associated to cardiovascular outcomes. The new Complior Analyse measures both PWV and central blood pressure during the same acquisition. The aim of this study was to compare PWV values from Complior Analyse with the previous Complior SP (PWVcs) and with Sphygmocor (PWVscr; AtCor, Australia), and to compare central systolic pressure from Complior Analyse and Sphygmocor. Peripheral and central pressures and PWV were measured with the three devices in 112 patients. PWV measurements from Complior Analyse were analysed using two foot-detection algorithms (PWVca_it and PWVca_cs). Both radial (ao-SBPscr) and carotid (car-SBPscr) approaches from Sphygmocor were compared to carotid Complior Analyse measurements (car-SBPca). The same distance and same calibrating pressures were used for all devices. PWVca_it was strongly correlated to PWVscr (R(2) = 0.93, P < 0.001) with a difference of 0.0 ± 0.7  m/s. PWVca_cs was also correlated to PWVcs (R(2) = 0.90, P < 0.001) with a difference of 0.1 ± 0.7  m/s. Central systolic pressures were strongly correlated. The difference between car-SBPca and ao-SBPscr was 3.1 ± 4.2  mmHg (P < 0.001), statistically equivalent to the difference between car-SBPscr and ao-SBPscr (3.9 ± 5.8  mmHg, P < 0.001), whilst the difference between car-SBPca and car-SBPscr was negligible (-0.7 ± 5.6  mmHg, P = NS). The new Complior Analyse device provides equivalent results for PWV and central pressure values to the Sphygmocor and Complior SP. It reaches Association for the Advancement of Medical Instrumentation standard for central blood pressure and grades as excellent for PWV on the Artery Society criteria. It can be interchanged with existing devices.

  5. RECURSO AO LEGISLADOR: CONSIDERAÇÕES EM TORNO DO CONTROLE LEGISLATIVO AO PODER JUDICIÁRIO (UM EPITÁFIO À PEC Nº33)

    OpenAIRE

    Dettmam, Deborah; Matos, Nelson Juliano Cardoso

    2017-01-01

    Este artigo discute se o recurso ao povo ou ao legislador, como instrumento de resolução dos conflitos entre os poderes, viola a separação de poderes e a independência do poder judiciário ou se, antes disso, dá fiel execução à separação de poderes. Para alcançar esse objetivo, esse artigo investiga se existe relação entre o recurso ao legislador e os regimes autoritários; se a Constituição Federal de 1988 proíbe outro guardião constitucional, que não o Supremo Tribunal Federal, e quais as con...

  6. Ab urbe condita. Desde a fundaçao da cidade de Bracara Augusta

    Directory of Open Access Journals (Sweden)

    Rui Morais

    2005-01-01

    Full Text Available Neste estudo aborda-se a problemática da fundaçao da antiga cidade de Bracara Augusta, levando em consideraçao o registro arqueológico e sua problemática e significado. A parte da fundaçao ex novo da cidade ou da preexistência de populaçoes autóctones no local, apresentam-se fortes vestígios indicadores da importância do culto imperial e dinástico que pensamos fortemente associados ao acto simbólico da sua fundaçao ou "refundaçao" segundo uma cerimónia à da "Roma Quadrada" augústea.

  7. Lactate dehydrogenase of Mugil sp. (Mugilidae, Perciformes. Lack of electrokinetic, thermostability and kinetic differences among individuals with different number of scales

    Directory of Open Access Journals (Sweden)

    Marcelo dos Santos

    2000-03-01

    ão desses locos da LDH. Não foi observada nenhuma associação entre padrão eletroforético, inativação térmica, respostas cinéticas e diferentes SNS. Os valores de Km (pyr aparente aqui obtidos mostraram-se similares aos obtidos por outros autores para músculo e coração, bem como aos de suas isoformas purificadas. O efeito do NaCl nos valores de Km e Vmax de Mugil sp. (SNS de 35 e 39 indica que esse sal se comporta como um inibidor competitivo, já que decresce a afinidade enzima-substrato. Assim, os comportamentos eletrocinéticos e de termoestabilidade, valores de Km e Vmax e os efeitos do NaCl sobre esses não nos permitem caracterizar as tainhas capturadas na região estuarino-lagunar de Cananéia, com amplitudes de SNS de 33 a 39, como diferentes espécies ou populações.

  8. Impact of serum SP-A and SP-D levels on comparison and prognosis of idiopathic pulmonary fibrosis

    Science.gov (United States)

    Wang, Kai; Ju, Qing; Cao, Jing; Tang, Wenze; Zhang, Jian

    2017-01-01

    Abstract Background and objective: Idiopathic pulmonary fibrosis (IPF) has a poor prognosis in general; however, it is heterogeneous to detect relative biomarkers for predicting the disease progression. Serum biomarkers can be conveniently collected to detect and help to differentially diagnose IPF and predict IPF prognosis. This meta-analysis aimed to evaluate the use of serum surfactant proteins A and D (SP-A and SP-D) for differential diagnosis and prognosis of IPF. Methods: Relevant articles were searched in PubMed, Embase, and Chinese National Knowledge Infrastructure databases and reviewed by 2 independent readers. Standard mean difference (SMD) and 95% confidence interval (CI) were calculated to assess the difference in serum levels of SP-A/D among patients with IPF, when compared to patients with non-IPF interstitial lung disease (ILD), pulmonary infection, and healthy control. Hazard ratio (HR) and 95% CI were used to compare the relative risk of mortality. Results: Twenty-one articles (totalling 1289 IPF patients) were included in final meta-analysis. Serum SP-A levels were significantly higher in patients with IPF than in patients with non-IPF ILD (SMD: 1.108 [0.584, 1.632], P infection (SMD: 1.320 [0.999, 1.640], P SMD: 2.802 [1.901, 3.702], P SMD: 0.459 [−0.000, 0.919], P = .050). Serum SP-D levels were significantly higher in patients with IPF than in patients with pulmonary infection (SMD: 1.308 [0.813, 1.803], P SMD: 2.235 [1.739, 2.731], P < .001). Risk of death in patients with IPF and elevated serum SP-A was increased 39% compared to patients with low SP-A groups. Elevated SP-D increased risk by 111% when compared to low SP-D. In acute exacerbation of IPF, serum SP-A/D were higher than those in stable stage. The comparisons and prognosis might be different in Asian and Caucasian patients. Conclusions: Serum SP-A/D detection might be useful for differential diagnosis and prediction of survival in patients with IPF. PMID:28591049

  9. The SpTransformer Gene Family (Formerly Sp185/333) in the Purple Sea Urchin and the Functional Diversity of the Anti-Pathogen rSpTransformer-E1 Protein

    Science.gov (United States)

    Smith, L. Courtney; Lun, Cheng Man

    2017-01-01

    The complex innate immune system of sea urchins is underpinned by several multigene families including the SpTransformer family (SpTrf; formerly Sp185/333) with estimates of ~50 members, although the family size is likely variable among individuals of Strongylocentrotus purpuratus. The genes are small with similar structure, are tightly clustered, and have several types of repeats in the second of two exons and that surround each gene. The density of repeats suggests that the genes are positioned within regions of genomic instability, which may be required to drive sequence diversification. The second exon encodes the mature protein and is composed of blocks of sequence called elements that are present in mosaics of defined element patterns and are the major source of sequence diversity. The SpTrf genes respond swiftly to immune challenge, but only a single gene is expressed per phagocyte. Many of the mRNAs appear to be edited and encode proteins with altered and/or missense sequence that are often truncated, of which some may be functional. The standard SpTrf protein structure is an N-terminal glycine-rich region, a central RGD motif, a histidine-rich region, and a C-terminal region. Function is predicted from a recombinant protein, rSpTransformer-E1 (rSpTrf-E1), which binds to Vibrio and Saccharomyces, but not to Bacillus, and binds tightly to lipopolysaccharide, β-1,3-glucan, and flagellin, but not to peptidoglycan. rSpTrf-E1 is intrinsically disordered but transforms to α helical structure in the presence of binding targets including lipopolysaccharide, which may underpin the characteristics of binding to multiple targets. SpTrf proteins associate with coelomocyte membranes, and rSpTrf-E1 binds specifically to phosphatidic acid (PA). When rSpTrf-E1 is bound to PA in liposome membranes, it induces morphological changes in liposomes that correlate with PA clustering and leakage of luminal contents, and it extracts or removes PA from the bilayer. The

  10. Iguaçu (IAC-21 e Araguaia (IAC -22: cultivares de trigo de sequeiro para o Estado de São Paulo Iguaçu (IAC 21 and Araguaia (IAC 22: wheat cultivars for upland conditions of the State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    João Carlos Felício

    1985-01-01

    Full Text Available Em experimentos localizados nas principais regiões tritícolas paulistas, comparou-se a produtividade e as reações aos agentes de ferrugem-do-colmo e da-folha, bem como as qualidades industriais de panificação de dois novos cultivares de trigo - Iguaçu (IAC-21 e Araguaia (IAC-22 - provenientes de cruzamentos artificiais e obtidos por seleção por método genealógico, com os cultivares BH-1146 e Maringá (IAC-5. A produção média de grãos do 'Iguaçu' foi 8% e 12% estatisticamente superior respectivamente aos cultivares testemunhas, e o 'Araguaia' superou-os em 5 e 14%. Os novos cultivares apresentaram, ao longo dos cinco anos de estudo, menores níveis de infecção de ferrugem-do-colmo (Puccinia gramínis f, sp. tritici que as testemunhas, sobressaindo-se o 'Araguaia', com baixíssimos níveis de infecção. Os novos cultivares, bem como as testemunhas, mostraram reações médias para ferrugem-da-folha. Nos ensaios de panificação a farinha do 'Araguaia', de maneira geral, exibiu melhor potencial panificável que a do Iguaçu, sendo ambas levemente superiores à farinha de trigo comercial.Two new selected germoplasms of wheat (Iguaçu and Araguaia were tested against two commercial varieties (BH-1146 and IAC-5 for yield and resistance to stem and leaf rusts in several experiments carried out in different locations of the wheat areas of the State of São Paulo, Brazil, in the period 1978-1982. Cultivar Iguaçu produced 8% and 12% more grain per hectare in comparison with respectively, cultivars BH-1146 and IAC-5; cultivar Araguaia had, respectively, 5% and 14% more grain yield than the same commercial varieties. Iguaçu and Araguaia cultivars presented less stem rust infection than BH-1146 and IAC-5 while Araguaia had a lower infection level than Iguaçu under field conditions. It was not observed differences in resistance to leaf rust among them. Araguaia exhibited better bread qualities than Iguaçu but both showed better

  11. Resposta ao estresse: II. Resiliência e vulnerabilidade

    Directory of Open Access Journals (Sweden)

    Nicole Leite Galvão-Coelho

    2015-06-01

    Full Text Available ResumoA crescente exposição a estressores na vida cotidiana aumentou significativamente a investigação da resposta ao estresse nas duas últimas décadas. Embora associada a consequências negativas, pois muitas patologias físicas e mentais são desencadeadas por exposição crônica a estressores, esta resposta é indispensável para sobrevivencia do indivíduo e é extremamente adaptativa quando ativada de forma aguda. Na parte I desta revisão foram abordados os conceitos de homestase e alostase e os sistemas fisiológicos ativados durante a resposta ao estresse. Na parte II serão discutidos fatores que modulam a resposta ao estresse tais como sexo, temperamento, períodos críticos do desenvolvimento e a presença ou ausência de suporte social. A interação entre os fatores genéticos e ambientais gera os perfis da resposta psicofisiológica que caracterizam os fenótipos de susceptibilidade e resiliência frente aos estressores e sua relação com uma patologia mental cada vez mais presente na sociedade moderna, o transtorno de estresse pós-traumático.

  12. Prevenção da Aids com adolescentes encarcerados em São Paulo, SP AIDS prevention among incarcerated teenagers, Brazil

    Directory of Open Access Journals (Sweden)

    Camila Alves Peres

    2002-08-01

    Full Text Available OBJETIVOS: Descrever o perfil de adolescentes quanto ao apoio social e familiar, ao uso de drogas e os conhecimentos, as práticas e atitudes relacionadas à Aids e sua prevenção. MÉTODOS: Foram estudados 275 jovens internos, do sexo masculino, de um centro de internação da Fundação Estadual do Bem Estar do Menor (Febem, em São Paulo, SP. A pesquisa foi feita em duas fases: a primeira por meio de entrevistas semi-estruturadas com 20 internos; a segunda, com questionários para auto-respostas aplicados aos 275 internos, com perguntas fechadas referentes a características sociodemográficas, criminalidade, práticas sexuais, uso de drogas, conhecimento, atitudes e práticas relativas à Aids. RESULTADOS: Do total estudado, 90% dos jovens internos residiam com suas famílias antes da internação; todos haviam estudado em escolas públicas, ainda que 61% já houvessem abandonado os estudos; 12% já haviam usado drogas; e 5,5% eram usuários de drogas intravenosas. A maioria (98% era sexualmente ativa; 35% haviam tido mais de 15 parceiras(os sexuais ao longo da vida; 8% haviam tido experiências homossexuais (dentro ou fora da Febem; 12% já haviam trocado sexo por benefícios materiais; e 22% já eram pais. Muitos dos adolescentes afirmaram que adquirir o HIV "é parte da vida" e que suas vidas apresentam riscos piores, como sobreviver na criminalidade. Acreditam que o preservativo é frágil (83% e atrapalha a relação sexual (58%; 72% já haviam utilizado preservativo, mas apenas 9% o utilizavam sempre. CONCLUSÕES: Os adolescentes apresentaram um elevado risco de aquisição do HIV. Assim, torna-se necessário integrar a prevenção da Aids em sua problemática de vida e em temas como racismo, esperança pelo futuro, criminalidade, uso de drogas, direitos fundamentais, incluídos nestes os referentes ao sexo e à reprodução, mostrando existir alternativas a adquirir o HIV ou morrer na criminalidade.OBJECTIVES: To describe social and

  13. Implementation of intermittent preventive treatment in pregnancy with sulphadoxine/pyrimethamine (IPTp-SP at a district health centre in rural Senegal

    Directory of Open Access Journals (Sweden)

    Vaillant Michel

    2008-11-01

    Full Text Available Abstract Background Intermittent preventive treatment with sulphadoxine-pyrimethamine (SP is recommended for reducing the risk of malaria in pregnancy and its consequences on mothers and babies (IPTp-SP. Indicators of implementation and effects of IPTp-SP were collected in a rural clinic in Southern Senegal. Methods Women seen routinely at the antenatal clinic (ANC of a rural dispensary during 2000–2007. Deployment of IPTp-SP started in January 2004. Inspection of antenatal and outpatient clinic registries of the corresponding period. Results Between 1st January 2000 and 30th April 2007, 1,781 women of all gravitidities and parities attended the ANC with 965 deliveries (606 and 398 respectively since 1st January 2004, when IPTp-SP was started. 69% of women were seen ≥ 3 times; 95% received at least one dose and 70% two doses of SP (from 61% in 2004 to 86% in 2007. The first visit, first and second dose of SP occurred at a median week 20, 22 and 31. The probability of receiving two doses was > 80% with ≥ 3 antenatal visits and a first dose of SP by week 20. The prevalence of maternal malaria was low and similar pre- (0.7% and during IPTp (0.8%. Effects on of low birth weight (LBW, Unfavourable pregnancy outcomes numbered 72 (7.5% of pregnancies with known outcome, including 30 abortions and 42 later deaths (late foetal deaths, stillbirth, peri-natal of which 13 with one or more malformations (1.35% of all recorded deliveries. Conclusion The implementation of IPTp-SP was high. Early attendance to ANC favours completion of IPTp-SP. The record keeping system in place is amenable to data extraction and linkage. A model was developed that predicts optimal compliance to two SP doses, and could be tested in other settings. Maternal malaria was infrequent and unaffected by IPTp-SP. The risk of LBW was lower during IPT implementation but the difference was non-significant and could have other explanations.

  14. Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula frente ao herpersvírus bovino tipo 1 (BoHV-1 e ao vírus da diarreia viral bovina (BVDV

    Directory of Open Access Journals (Sweden)

    Cristina Mendes Peter

    relação a marrom (log = 3,5log e de jataí (log = 3,24log. No entanto, frente ao BVDV a própolis jataí apresentou os melhores resultados em ambas as temperaturas (22oC e 37oC. Portanto, os extratos avaliados apresentaram atividade antiviral e virucida frente ao BoHV-1 e BVDV, o que os torna alvo para o desenvolvimento de novos biofármacos como alternativa ao uso de antivirais comerciais em Medicina Veterinária.

  15. Subcritical Water Technology for Extraction of Phenolic Compounds from Chlorella sp. Microalgae and Assessment on Its Antioxidant Activity.

    Science.gov (United States)

    Zakaria, Siti Maisurah; Kamal, Siti Mazlina Mustapa; Harun, Mohd Razif; Omar, Rozita; Siajam, Shamsul Izhar

    2017-07-03

    Chlorella sp . microalgae is a potential source of antioxidants and natural bioactive compounds used in the food and pharmaceutical industries. In this study, a subcritical water (SW) technology was applied to determine the phenolic content and antioxidant activity of Chlorella sp . This study focused on maximizing the recovery of Chlorella sp. phenolic content and antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay as a function of extraction temperature (100-250 °C), time (5-20 min) and microalgae concentration (5-20 wt. %) using response surface methodology. The optimal operating conditions for the extraction process were found to be 5 min at 163 °C with 20 wt. % microalgae concentration, which resulted in products with 58.73 mg gallic acid equivalent (GAE)/g phenolic content and 68.5% inhibition of the DPPH radical. Under optimized conditions, the experimental values were in close agreement with values predicted by the model. The phenolic content was highly correlated (R² = 0.935) with the antioxidant capacity. Results indicated that extraction by SW technology was effective and that Chlorella sp . could be a useful source of natural antioxidants.

  16. Análise da produtividade científica dos docentes da Universidade Estadual Paulista, campus de Marília/SP

    Directory of Open Access Journals (Sweden)

    Rondinelli Donizetti Herculano

    Full Text Available O objetivo do presente trabalho foi analisar/quantificar a produtividade científica dos docentes pertencentes à Universidade de Estadual Paulista (UNESP, campus de Marília/SP. Para cada professor, analisamos o número total de artigos na plataforma Lattes, no ISI Web of Science e no SCOPUS. Além disso, verificamos o total de livros publicados, capítulos de livros publicados e orientações em Programas de Pós-Graduação (PPG. A análise dos nove departamentos da Faculdade de Filosofia e Ciências (FFC mostrou que o Departamento de Ciências Políticas e Econômicas possui uma média elevada (21,42 de artigos na Plataforma Lattes, quando comparado aos outros departamentos. Entretanto, o Departamento de Fonoaudiologia apresentou médias mais altas em artigos indexados pelo ISI (2,61 e SCOPUS (4,39, bem como o índice-h, nessas plataformas. Em relação aos programas de pós-graduação, o Departamento de Administração e Supervisão Escolar foi o que apresentou a maior média de orientações em andamento e/ou concluídas. O desempenho mais baixo, em relação aos artigos publicados pelo ISI e SCOPUS, pelos departamentos na área de Humanas/Humanidades pode estar associado às características do campo da concentração e da cobertura limitada do banco de dados ISI e SCOPUS, indicando que o índice-h é muito sensível ao campo de concentração.

  17. [EFFICIENCY OF INTRODUCING CAROTENE PRODUCING STRAINS BACILLUS SP. 1.1 AND B. AMYLOLIQUEFACIENS UCM B-5113 INTO THE CHIKENS DIET].

    Science.gov (United States)

    Nechypurenko, O O; Kharhota M A; Avdeeva, L V

    2015-01-01

    It was shown the efficiency of carotene producing strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 in the diet of chickens. Also it was detected the lowering of the quantitative content of bacterial genera Enterococcus, Staphylococcus, family Enterobacteriaceae in the gut after eating by chickens cross "H&N Brown Nick" fodder with strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 alone and in composition in quantities 1 x 10(10) CFU per 1 g of feed. On the 18th day after introduction of cultures Bacillus sp. 1.1, B. amyloliquefaciens UCM B-5113 and their composition in the diet of poultry we revealed the increasing of body weight by 21.6, 7.6 and 22.0%, respectively, comparesing to controls. Also due to Bacillus sp. 1.1 it was detected the restore of intestinal villous structures, tissues of spleen, liver and heart. We found the additive effect of the composition of the investigated strains of bacteria genus Bacillus to the chickens.

  18. Adesão ao tratamento da ambliopia

    OpenAIRE

    Arakaki,Mara Regina; Schellini,Silvana Artioli; Heimbeck,Felipe Jorge; Furuya,Margareth Tiemi; Padovani,Carlos Roberto

    2004-01-01

    OBJETIVO: Avaliar a influência da adesão no resultado do tratamento da ambliopia. MÉTODOS: Foi feito estudo retrospectivo avaliando a resposta ao tratamento da ambliopia em 151 crianças portadoras de ambliopia, tratadas com esquema de oclusão diária, com o número de dias variando de acordo com a idade da criança. Os pacientes foram separados em dois grupos que diferiam entre si pela adesão ou não ao tratamento. RESULTADOS: Observou-se que o tratamento teve melhores resultados nas crianças de ...

  19. Taxonomy Icon Data: Synechocystis sp.PCC 6803 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Synechocystis sp.PCC 6803 Synechocystis sp.PCC 6803 Synechocystis_sp_PCC_6803_L.png Synecho...cystis_sp_PCC_6803_NL.png Synechocystis_sp_PCC_6803_S.png Synechocystis_sp_PCC_6803_NS.png http://bi...osciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synecho...cystis+sp%2ePCC+6803&t=NL http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synecho...cystis+sp%2ePCC+6803&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis

  20. Gilliamella intestini sp. nov., Gilliamella bombicola sp. nov., Gilliamella bombi sp. nov. and Gilliamella mensalis sp. nov.: Four novel Gilliamella species isolated from the bumblebee gut.

    Science.gov (United States)

    Praet, Jessy; Cnockaert, Margo; Meeus, Ivan; Smagghe, Guy; Vandamme, Peter

    2017-06-01

    Spectra of five isolates (LMG 28358 T , LMG 29879 T , LMG 29880 T , LMG 28359 T and R-53705) obtained from gut samples of wild bumblebees of Bombus pascuorum, Bombus lapidarius and Bombus terrestris were grouped into four MALDI-TOF MS clusters. RAPD analysis revealed an identical DNA fingerprint for LMG 28359 T and R-53705 which also grouped in the same MALDI-TOF MS cluster, while different DNA fingerprints were obtained for the other isolates. Comparative 16S rRNA gene sequence analysis of the four different strains identified Gilliamella apicola NCIMB 14804 T as nearest neighbour species. Average nucleotide identity values of draft genome sequences of the four isolates and of G. apicola NCIMB 14804 T were below the 96% threshold value for species delineation and all four strains and G. apicola NCIMB 14804 T were phenotypically distinct. Together, the draft genome sequences and phylogenetic and phenotypic data indicate that the four strains represent four novel Gilliamella species for which we propose the names Gilliamella intestini sp. nov., with LMG 28358 T as the type strain, Gilliamella bombicola sp. nov., with LMG 28359 T as the type strain, Gilliamella bombi sp. nov., with LMG 29879 T as the type strain and Gilliamella mensalis sp. nov., with LMG 29880 T as the type strain. Copyright © 2017 Elsevier GmbH. All rights reserved.

  1. EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill

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    SARMILA TASNIM

    2013-05-01

    Full Text Available Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%.

  2. Rhodotorula rosulata sp. nov., Rhodotorula silvestris sp. nov. and Rhodotorula straminea sp. nov., novel myo-inositol-assimilating yeast species in the Microbotryomycetes.

    Science.gov (United States)

    Golubev, Wladyslav I; Scorzetti, Gloria

    2010-10-01

    Three novel species are described as Rhodotorula rosulata sp. nov. (type strain VKM Y-2962(T) =CBS 10977(T)), Rhodotorula silvestris sp. nov. (type strain VKM Y-2971(T) =CBS 11420(T)) and Rhodotorula straminea sp. nov. (type strain VKM Y-2964(T) =CBS 10976(T)) based on the study of eight isolates from needle litter. The new species, phylogenetically located within the Microbotryomycetes, are related to glucuronate-assimilating species of the genus Rhodotorula. Sequencing of the D1/D2 domains of the LSU rDNA gene and the internal transcribed spacer (ITS) region, as well as physiological characterization, revealed their distinct taxonomic positions.

  3. Pre-analise das condições atuais de degradação das microbacias dos rios Itaim-Guaçu, Braiaia e Pirapitingui, Itu-SP

    OpenAIRE

    Liziane de Rosso Eymael

    2005-01-01

    Resumo: Uma expressiva modificação ambiental onde o impacto das atividades humanas tem resultado em danos aos recursos hídricos, vem sendo verificada nas microbacias dos rios Itaim-Guaçu, Pirapitingui e Braiaiá, em Itu, SP, principais mananciais superficiais do município. Dessa forma justifica-se a necessidade de serem desenvolvidos programas, ferramentas políticas e de gerenciamento ambiental que busquem uma redução desta degradação. O presente trabalho tem por objetivo fazer uma pré-análise...

  4. NCBI nr-aa BLAST: CBRC-TNIG-22-0186 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0186 ref|NP_000669.1| alpha-1D-adrenergic receptor [Homo sapiens] sp|P...25100|ADA1D_HUMAN Alpha-1D adrenergic receptor (Alpha 1D-adrenoceptor) (Alpha 1D-adrenoreceptor) (Alpha-1A adrenergic... receptor) (Alpha adrenergic receptor 1a) gb|AAB60351.1| adrenergic alpha-1a receptor protein gb|AAB59487.1| alpha 1a/d adre...nergic receptor dbj|BAA06222.1| alpha1A/D adrenergic rec...eptor [Homo sapiens] emb|CAH70478.1| adrenergic, alpha-1D-, receptor [Homo sapiens] emb|CAC00601.2| adrenergic

  5. NCBI nr-aa BLAST: CBRC-TNIG-22-0228 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0228 ref|NP_940906.1| progestin and adipoQ receptor family member IX [...Homo sapiens] ref|XP_526335.1| PREDICTED: hypothetical protein [Pan troglodytes] sp|Q6ZVX9|PAQR9_HUMAN Progesti...n and adipoQ receptor family member 9 (Progestin and adipoQ receptor family member IX) dbj|BAC85729.1| un...named protein product [Homo sapiens] gb|AAR08375.1| progestin and adipoQ receptor... family member IX [Homo sapiens] gb|AAI18667.1| Progestin and adipoQ receptor family member IX [Homo sapiens] gb|AAI22528.1| Progesti

  6. Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles.

    Science.gov (United States)

    Margos, Gabriele; Lane, Robert S; Fedorova, Natalia; Koloczek, Johannes; Piesman, Joseph; Hojgaard, Andrias; Sing, Andreas; Fingerle, Volker

    2016-03-01

    Two species of the genus Borrelia , Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov., were first described by Postic and co-workers on the basis of genetic analyses of several loci. Multilocus sequence analysis of eight housekeeping loci confirmed that these two Borrelia genomospecies are distinct members of the Borrelia burgdorferi sensu lato complex. B. bissettiae sp. nov. was initially described in transmission cycles involving Neotoma fuscipes wood rats and Ixodes pacificus ticks in California, and Neotoma mexicana and Ixodes spinipalpis in Colorado. The preferred host of B. californiensis sp. nov. appears to be the California kangaroo rat, Dipodomys californicus ; Ixodes jellisoni , I. spinipalipis and I. pacificus ticks are naturally infected with it. Thus, the ecological associations of the two genomospecies and their genetic distance from all other known Borrelia genomospecies species justify their description as separate genomospecies: B. bissettiae sp. nov. (type strain DN127 T  = DSM 17990 T  =  CIP 109136 T ) and B. californiensis (type strain CA446 T  = DSM 17989 T  = ATCC BAA-2689 T ).

  7. Pengaruh Perbedaan Konsentrasi Ekstrak Sargassum sp. dan Lama Penyimpanan terhadap Oksidasi Lemak pada Fillet Ikan Patin (Pangasius sp.

    Directory of Open Access Journals (Sweden)

    Fatin Hidayati

    2017-05-01

    Full Text Available ABSTRAK Ikan patin merupakan ikan air tawar yang mengandung lemak dan protein tinggi sehingga apabila dilakukan penyimpanan rentan terjadi oksidasi yang mengakibatkan ketengikan. Sargassum sp. dengan kandungan fenol dan flavonoid mampu menghambat terjadinya oksidasi pada fillet ikan patin. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan ekstrak Sargassum sp. dan lama penyimpanan dalam menghambat terjadinya oksidasi pada fillet ikan patin. Materi yang digunakan dalam penelitian ini adalah ekstrak Sargassum sp. dan fillet ikan patin. Metode penelitian yang digunakan adalah experimental laboratories dengan menggunakan Rancangan Acak Lengkap (RAL faktorial dengan 2 faktor yaitu konsentrasi ekstrak Sargassum sp. (0%, 1%, 1,5% dan 2% dan lama penyimpanan (hari ke-0, hari ke-2, hari ke-4, dan hari ke-6. Hasil penelitian menunjukkan bahwa perbedaan penambahan konsentrasi ekstrak Sargassum sp. dan lama penyimpanan memberikan pengaruh nyata terhadap nilai PV, nilai TBA, kadar lemak, kadar protein, kadar air serta organoleptik (P < 0,05. Hasil penelitian tahap I didapatkan rendemen Sargassum sp. dengan pelarut etanol 96% sebesar 1,39%, kandungan fenol 1,813%, flavonoid 0,278% dan aktivitas antioksidan 99,1659 ppm (kuat. Hasil penelitian tahap II didapatkan nilai PV berkisar antara 2,03 - 19,82 meq/kg, nilai TBA 0,63 - 6,72 mg.mal/kg. Konsentrasi 1,5% merupakan konsentrasi terbaik ekstrak Sargassum sp. dalam menghambat oksidasi lemak pada fillet ikan patin selama penyimpanan. Kata kunci: Antioksidan, Ekstrak Sargassum sp., Lama Penyimpanan, Oksidasi lemak, Fillet Ikan patin ABSTRACT Catfish is a freshwater fish that contain high fat and protein so that if its stored it will susceptible to oxidation process which leads to rancidity. Sargassum sp. with its phenolic and flavonoid content are able to inhibit the oxidation process in catfish fillet. This research was aimed to know the effects of different concentrations of Sargassum sp. extracts and

  8. Pretrichodermamides D-F from a Marine Algicolous Fungus Penicillium sp. KMM 4672.

    Science.gov (United States)

    Yurchenko, Anton N; Smetanina, Olga F; Ivanets, Elena V; Kalinovsky, Anatoly I; Khudyakova, Yuliya V; Kirichuk, Natalya N; Popov, Roman S; Bokemeyer, Carsten; von Amsberg, Gunhild; Chingizova, Ekaterina A; Afiyatullov, Shamil Sh; Dyshlovoy, Sergey A

    2016-06-27

    Three new epidithiodiketopiperazines pretrichodermamides D-F (1-3), together with the known N-methylpretrichodermamide B (4) and pretrichodermamide С (5), were isolated from the lipophilic extract of the marine algae-derived fungus Penicillium sp. KMM 4672. The structures of compounds 1-5 were determined based on spectroscopic methods. The absolute configuration of pretrichodermamide D (1) was established by a combination of modified Mosher's method, NOESY data, and biogenetic considerations. N-Methylpretrichodermamide B (5) showed strong cytotoxicity against 22Rv1 human prostate cancer cells resistant to androgen receptor targeted therapies.

  9. Fatores associados à atividade física e aos comportamentos sedentários em adolescentes

    Directory of Open Access Journals (Sweden)

    Daniel Giordani Vasques

    2009-01-01

    Full Text Available http://dx.doi.org/10.5007/1980-0037.2009v11n1p59   Objetivou-se analisar fatores associados à atividade física e aos comportamentos sedentários em adolescentes. A amostra foi composta de 1675 escolares (784 rapazes e 891 moças, de 11 a 17 anos de idade, de Caxias do Sul-RS. Um questionário foi aplicado para identificar o nível de atividade física (recordatório de 3 dias e o número de horas em comportamentos sedentários. Considerou-se como baixo nível de atividade física (baixo NAF um gasto energético menor do que 37kcal/kg/dia e exposição elevada a comportamentos sedentários (CSE assistir TV, jogar videogame ou utilizar computador mais do que 14h/semana. Os resultados do teste qui-quadrado indicaram que as moças apresentaram maior prevalência de baixo NAF (66,8%>43,2%; p84,0%; p=0,001. Baixo NAF associou-se aos rapazes a partir da regressão de Poisson, com residir com até 4 pessoas (RP=1,21;IC95%1,00-1,46 e com o baixo NAF da mãe (RP=1,23;IC95%1,00-1,53, e nas moças, com a faixa etária de 15-17 anos (RP=1,30;IC95%1,18-1,44, residir com até 4 pessoas (RP=1,17;IC95%1,04-1,31, possuir TV no quarto (RP=1,13;IC95%1,02-1,25 e deslocar-se passivamente à escola (RP=1,10;IC95%1,00-1,22. A CSE associou-se às moças à maior escolaridade do chefe da família (RP=1,08;IC95%1,01-1,16 e a possuir TV no quarto (RP=1,15;IC95%1,08-1,22. Destaca-se a associação entre as medidas de aproximação socioeconômica e os comportamentos de risco (baixo NAF e CSE. O conhecimento dos fatores associados ao baixo NAF e à CSE facilita a implantação de intervenções mais efetivas, a fim de promover um estilo de vida mais ativo.

  10. Scalability of Parallel Spatial Direct Numerical Simulations on Intel Hypercube and IBM SP1 and SP2

    Science.gov (United States)

    Joslin, Ronald D.; Hanebutte, Ulf R.; Zubair, Mohammad

    1995-01-01

    The implementation and performance of a parallel spatial direct numerical simulation (PSDNS) approach on the Intel iPSC/860 hypercube and IBM SP1 and SP2 parallel computers is documented. Spatially evolving disturbances associated with the laminar-to-turbulent transition in boundary-layer flows are computed with the PSDNS code. The feasibility of using the PSDNS to perform transition studies on these computers is examined. The results indicate that PSDNS approach can effectively be parallelized on a distributed-memory parallel machine by remapping the distributed data structure during the course of the calculation. Scalability information is provided to estimate computational costs to match the actual costs relative to changes in the number of grid points. By increasing the number of processors, slower than linear speedups are achieved with optimized (machine-dependent library) routines. This slower than linear speedup results because the computational cost is dominated by FFT routine, which yields less than ideal speedups. By using appropriate compile options and optimized library routines on the SP1, the serial code achieves 52-56 M ops on a single node of the SP1 (45 percent of theoretical peak performance). The actual performance of the PSDNS code on the SP1 is evaluated with a "real world" simulation that consists of 1.7 million grid points. One time step of this simulation is calculated on eight nodes of the SP1 in the same time as required by a Cray Y/MP supercomputer. For the same simulation, 32-nodes of the SP1 and SP2 are required to reach the performance of a Cray C-90. A 32 node SP1 (SP2) configuration is 2.9 (4.6) times faster than a Cray Y/MP for this simulation, while the hypercube is roughly 2 times slower than the Y/MP for this application. KEY WORDS: Spatial direct numerical simulations; incompressible viscous flows; spectral methods; finite differences; parallel computing.

  11. Low prevalence of Blastocystis sp. in active ulcerative colitis patients.

    Science.gov (United States)

    Rossen, N G; Bart, A; Verhaar, N; van Nood, E; Kootte, R; de Groot, P F; D'Haens, G R; Ponsioen, C Y; van Gool, T

    2015-05-01

    Ulcerative colitis (UC) is thought to originate from a disbalance in the interplay between the gut microbiota and the innate and adaptive immune system. Apart from the bacterial microbiota, there might be other organisms, such as parasites or viruses, that could play a role in the aetiology of UC. The primary objective of this study was to compare the prevalence of Blastocystis sp. in a cohort of patients with active UC and compare that to the prevalence in healthy controls. We studied patients with active UC confirmed by endoscopy included in a randomised prospective trial on the faecal transplantation for UC. A cohort of healthy subjects who served as donors in randomised trials on faecal transplantation were controls. Healthy subjects did not have gastrointestinal symptoms and were extensively screened for infectious diseases by a screenings questionnaire, extensive serologic assessment for viruses and stool analysis. Potential parasitic infections such as Blastocystis were diagnosed with the triple faeces test (TFT). The prevalence of Blastocystis sp. were compared between groups by Chi-square testing. A total of 168 subjects were included, of whom 45 had active UC [median age 39.0 years, interquartile range (IQR) 32.5-49.0, 49 % male] and 123 were healthy subjects (median age 27 years, IQR 22.0-37.0, 54 % male). Blastocystis sp. was present in the faeces of 40/123 (32.5 %) healthy subjects and 6/45 (13.3 %) UC patients (p = 0.014). Infection with Blastocystis is significantly less frequent in UC patients as compared to healthy controls.

  12. ESTRATÉGIAS DE MARKETING NA INDÚSTRIA DE ALIMENTOS: O CASO DOS FRIGORÍFICOS NA REGIÃO DE PRESIDENTE PRUDENTE-SP

    Directory of Open Access Journals (Sweden)

    João Guilherme de Camargo Ferraz Machado

    2009-11-01

    Full Text Available O objetivo desse trabalho foi analisar as estratégias de marketing e comunicação dos frigoríficos na região de Presidente Prudente-SP, visando melhorar a competitividade do setor e de toda a cadeia produtiva, identificando as principais estratégias relacionadas ao composto mercadológico. A coleta de dados se deu por meio de entrevistas semi-estruturadas, aplicadas em dois frigoríficos da região de Presidente Prudente-SP. Observou-se que as estratégias do composto mercadológico das empresas do setor sofrem poucas variações, em função das características dos produtos e do público-alvo dessas empresas. Também foi verificado um distanciamento entre as empresas e o consumidor final, no que diz respeito às práticas de marketing e comunicação, podendo ser responsável por um desconhecimento, por parte das empresas, das necessidades e dos hábitos de consumo do consumidor final.

  13. A sp2+sp3 hybridized carbon allotrope transformed from AB stacking graphyne and THD-graphene

    Directory of Open Access Journals (Sweden)

    Yu Zhang

    2018-01-01

    Full Text Available New carbon allotropes can be designed by combining sp, sp2 and sp3 three hybridization states. And the hybridization states or coordination numbers of carbon atoms can be changed by applying high pressure on carbon materials. In this study, a common high pressure phase (named as TBBC transformed from AB-stacking graphyne or THD-graphene is predicted. Its kinetic stability is examined using finite displacement method. We find that the sp2 and sp3 hybridized carbon atoms behave different vibration features at high frequency region. Both graphene-like and diamond-like vibration peaks occurs. Phase transition energy barriers from both graphyne and THD-graphene to TBBC are estimated. Electronic structure calculations show that the TBBC is an indirect semiconductor with a bandgap of 0.66 eV. The ideal tensile strength of TBBC is high in [0001] and [11¯00] directions, but is weak along [12¯10] direction.

  14. Turbellaria from Curaçao

    NARCIS (Netherlands)

    Marcus, Ernst

    1960-01-01

    A study of the several fresh and brackish-water springs on Curaçao, carried out by Dr. P. WAGENAAR HUMMELINCK, revealed (a) the common occurrence on that island of Dugesia festai (Borelli 1898), a species which until then had only been known from the South American mainland (nearest find: Ecuador!),

  15. Purificación parcial y caracterización de una sustancia antimicrobiana producida por Alteromonas sp. de origen marino

    Directory of Open Access Journals (Sweden)

    Jorge León

    2013-05-01

    Full Text Available Un miembro del género Alteromonas sp. (cepa N22.C, aislado del neuston marino y productor de sustancias que inhiben el crecimiento de ictiopatógenos fue caracterizado fenotípicamente mediante pruebas morfológicas, fisiológicas y bioquímicas convencionales. Para determinar la naturaleza quí- mica de la sustancia inhibitoria, extractos crudos de sobrenadantes de cultivos fueron precipitados con concentraciones crecientes de Sulfato de Amonio hasta el 70% y filtrados a través de columnas de Sephadex G-25. Ensayos con Dodecil Sulfato de Sodio-electroforesis en Geles de Poliacrilamida (SDS– PAGE revelaron que la sustancia antimicrobiana es un compuesto proteináceo con una masa molecular de aproximadamente 34000 Da, que carece de residuos de azúcares asociados. Otros ensayos realizados con extractos crudos y fracciones semipurificadas de la cepa de Alteromonas sp. N22.C mostraron un amplio espectro de actividad antibiótica contra cepas de bacterias patógenas de peces, moluscos y crustáceos.

  16. Dias ao Parto de Fêmeas Nelore de um Experimento de Seleção para Crescimento: I - Modelo de Repetibilidade

    Directory of Open Access Journals (Sweden)

    Mercadante Maria Eugênia Zerlotti

    2002-01-01

    Full Text Available Registros de datas de entrada na monta e respectiva data do parto, referentes a 1.247 fêmeas Nelore dos rebanhos experimentais da Estação Experimental de Zootecnia de Sertãozinho (IZ - SP, selecionadas para altos (seleção e tradicional e para médios (controle pesos ao sobreano foram usados para obter a variável dias ao parto, a fim de estudar o efeito da seleção para crescimento sobre o desempenho reprodutivo. Arquivos de novilhas e de vacas e novilhas foram analisados incluindo e não incluindo as não paridas. Nenhuma diferença significativa foi detectada entre os registros provenientes das vacas dos rebanhos selecionados e do controle, apesar das vacas do rebanho seleção apresentarem as maiores médias de dias ao parto na maioria dos arquivos estudados. Concordando com os resultados obtidos para o efeito de rebanho, o peso à seleção foi significativo somente para as vacas e novilhas, considerando as não paridas, com tendência das mais pesadas à seleção apresentarem menores valores para dias ao parto. Modelos nos quais não foi considerado o peso à seleção forneceram os mesmos resultados para o efeito de rebanho. As herdabilidades variaram de 0,02 a 0,16, sendo as mais altas obtidas em arquivos nos quais foram incluídos os registros das não paridas, indicando que a observação de caracteres de reprodução somente das fêmeas férteis contribui para mascarar as diferenças genéticas entre os animais, e quando esta variabilidade é re-introduzida, designando-se penalidades às fêmeas que não pariram, as diferenças genéticas entre os animais aparecem. Existem evidências que a seleção para peso não comprometeu o desempenho reprodutivo das fêmeas, mesmo sendo criadas em condições ambientais similares.

  17. Biosorption characteristics of copper (II), chromium (III), nickel (II), and lead (II) from aqueous solutions by Chara sp. and Cladophora sp.

    Science.gov (United States)

    Elmaci, Ayşe; Yonar, Taner; Ozengin, Nihan

    2007-09-01

    The aim of this research was to expose individual removals of copper, chromium, nickel, and lead from aqueous solutions via biosorption using nonliving algae species, Chara sp. and Cladophora sp. Optimum pH values for biosorption of copper (II), chromium (III), nickel (II), and lead (II) from aqueous solutions were determined to be 6, 7, 7, and 3 for Cladophora sp. and 5, 3, 5, and 4 for Chara sp. respectively. Maximum adsorption capacities of Chara sp. [10.54 for chromium (III) and 61.72 for lead (II)] and Cladophora sp. [6.59 for chromium (III) and 16.75 and 23.25 for lead (II)] for chromium (III) and lead (II) are similar. On the other hand, copper (II) and nickel (II) biosorption capacity of Cladophora sp. [14.28 for copper (II) and 16.75 for nickel (II)] is greater than Chara sp. [6.506 for copper (II) and 11.76 for nickel (II)]. Significantly high correlation coefficients indicated for the Langmuir adsorption isotherm models can be used to describe the equilibrium behavior of copper, chromium, nickel, and lead adsorption onto Cladophora sp. and Chara sp.

  18. Tolerância ao florescimento precoce e características comerciais de progênies F4 de alface do cruzamento Regina 71 x Salinas 88 = Tolerance to precocious flowering and commerical traits of F4 progenies of hybrid Regina 71 x Salinas 88 lettuce

    Directory of Open Access Journals (Sweden)

    José Luiz Sandes de Carvalho Filho

    2009-01-01

    Full Text Available A alface é uma espécie cujas plantas possuem grande variabilidade no que diz respeito à forma, cor e textura das folhas, caracterizando diferentes tipos comerciais. O grupo folhas lisas ainda se mantém como um dos mais consumidos. A caracterização do tipo de folha é essencial no desenvolvimento de um programa de melhoramento,principalmente, quando a população segregante é oriunda de parentais contrastantes para o caráter. Além disso, a maior parte das cultivares utilizada é sensível ao calor, emitindo a haste floral precocemente. Assim, o objetivo deste trabalho foi avaliar características comercias de progênies F4 de alface e a sua tolerância ao calor, visando obter cultivares com folha lisa tolerantes ao florescimento precoce, resistentes aos nematóides e ao LMV. Avaliaram-se 22 progênies F4 originadas do cruzamento envolvendo as cultivares ‘Regina 71’ e ‘Salinas 88’, previamente selecionadas para tolerância ao calor, resistência aos nematóides das galhas e ao LMV (lettuce mosaic virus. O delineamento foi em blocos casualizados, com quatro repetições. Foram avaliados os tipos de limbo e borda foliares, formação de cabeça e número de dias para a primeira antese. Dentre as 22 progênies avaliadas, três mostraram-semais promissoras à obtenção de linhagens de alface de folhas lisas resistentes ao calor, nematóides das galhas e ao LMV.The lettuce is a plant that features great variability in form, color and texture. The butterleaf variety had remained one of themost consumed lettuce types. The evaluation of leaf type for commercial characters is essential for the development of any improvement program, especially when the parents are divergent types. Furthermore, most cultivars are susceptible to heat. Thus, the objective of the present work was to evaluate F4 progenies of butterleaf lettuce, aiming to select new genotypes for nematode resistance, tolerance to heat and LMV. Twenty-two F3:4 progenieswere

  19. Biological control agents (Arthropoda - Insecta associated with cultivation of the sugar apple (Annona squamosa L. = Agentes de controle biológico (Arthropoda - Insecta associados ao cultivo da pinha (Annona squamosa L.

    Directory of Open Access Journals (Sweden)

    Laise Malaspina Rossi

    2017-03-01

    Full Text Available Knowledge of biological control agents occurring naturally in agricultural areas is of great importance in adopting methods of integrated pest management. The aim of this work therefore, was to undertake a population survey of biological control agents (Insecta associated with cultivation of Annona squamosa. The research was carried out during the 2015/2016 harvest in a conventional orchard of A. squamosa, located in the town of Tabatinga in the State of São Paulo, Brazil. The biological control agents were captured using yellow sticky traps. The following faunistic indices were calculated: dominance, abundance, frequency and constancy. Population fluctuations in the natural enemies sampled were analysed by histogram. The coccinellid predators, Scymnus sp. (Coleoptera: Coccinellidae and Cycloneda sanguinea (Coleoptera: Coccinellidae were the principal biological control agents sampled during the study, and considered superdominant, superabundant, superfrequent and constant. As for population fluctuation of predominant natural enemies, high population density was seen in Scymnus sp. between September and January 2015, whereas there were three population peaks for C. sanguinea during the study, in September 2015 and January and July 2016. The coccinellids, Scymnus sp. and C. sanguinea, were the predominant biological control agents in the cultivation of A. squamosa, with a higher population density for the genus Scymnus seen in the spring and summer seasons, and no distribution pattern for C. sanguinea observed. = O conhecimento acerca dos agentes de controle biológico presentes naturalmente em áreas agrícolas apresenta grande importância na adoção de metodologias voltadas ao manejo integrado de pragas. Assim, objetivou-se com este trabalho realizar o levantamento da ocorrência populacional de agentes de controle biológico (Insecta associado ao cultivo de Annona squamosa. A pesquisa foi realizada na safra agrícola 2015/2016 em pomar

  20. Bacteriophage SP6 encodes a second tailspike protein that recognizes Salmonella enterica serogroups C{sub 2} and C{sub 3}

    Energy Technology Data Exchange (ETDEWEB)

    Gebhart, Dana; Williams, Steven R.; Scholl, Dean, E-mail: dean@avidbiotics.com

    2017-07-15

    SP6 is a salmonella phage closely related to coliphage K1-5. K1-5 is notable in that it encodes two polysaccharide-degrading tailspike proteins, an endosialidase that allows it to infect E. coli K1, and a lyase that enables it to infect K5 strains. SP6 is similar to K1-5 except that it encodes a P22-like endorhamnosidase tailspike, gp46, allowing it to infect group B Salmonella. We show here that SP6 can also infect Salmonella serogroups C{sub 2} and C{sub 3} and that a mutation in a putative second tailspike, gp47, eliminates this specificity. Gene 47 was fused to the coding region of the N-terminal portion of the Pseudomonas aeruginosa R2 pyocin tail fiber and expressed in trans such that the fusion protein becomes incorporated into pyocin particles. These pyocins, termed AvR2-SP47, killed serogroups C{sub 2} and C{sub 3}Salmonella. We conclude that SP6 encodes two tail proteins providing it a broad host range among Salmonella enterica. - Highlights: • SP6 is a “dual specificity” bacteriophage that encodes two different receptor binding proteins giving it a broad host range. • These receptor binding proteins can be used to re-target the spectrum of R-type bacteriocins to Salmonella enterica. • Both SP6 and the engineered R-type bacteriocins can kill the Salmonella serovars most associated with human disease making them attractive for development as antimicrobial agents.

  1. Barreiras ao tratamento da hipertensão arterial

    Directory of Open Access Journals (Sweden)

    Maria Vilani Cavalcante Guedes

    2011-12-01

    Full Text Available Objetivou-se descrever as barreiras encontradas pelas pessoas portadoras de hipertensão arterial para a não adesão ao tratamento e controle dos níveis de sua pressão arterial. Estudo descritivo e transversal realizado em seis unidades básicas de saúde em Fortaleza-Ceará, Brasil, com 246 pessoas inscritas no Programa de Controle de Hipertensão Arterial há, no mínimo, um ano. Coletaram-se dados com entrevista estruturada e no prontuário eletrônico. Identificaram-se 69 pesquisados com níveis pressóricos normalizados. As principais barreiras apontadas foram: baixas condições financeiras, tratamento contínuo com muitos remédios e prática de atividade física. Concluiu-se que as barreiras ao tratamento anti-hipertensivo compreendem a pessoa adoecida, seu ambiente de vida e acesso aos cuidados de saúde.

  2. Nemerteans from Curaçao

    NARCIS (Netherlands)

    Corrêa, Diva Diniz

    1963-01-01

    All species treated in this paper were collected by me between January and June 1962, during my stay at the “Caraïbisch Marien-Biologisch Instituut” (Caribbean Marine Biological Institute), Curaçao. I am deeply indebted to the Government of the Netherlands, which awarded me a grant enabling me to

  3. ESTUDOS ETNOBOTÂNICOS E AULAS SOBRE PLANTAS MEDICINAIS NA E.E. MARIA LUIZA DE GUIMARAES MEDEIROS, SÃO JOSÉ DOS CAMPOS - SP

    Directory of Open Access Journals (Sweden)

    Elisa Celeste Dreux

    2017-01-01

    Full Text Available Resumo - O presente trabalho objetivou investigar o conhecimento etnobotânico dos alunos da E.E. Maria Luiza de Guimarães Medeiros, localizado na região Norte de São José dos Campos – SP e paralelamente desenvolver aulas práticas com os alunos participantes do projeto PIBID, relacionadas ao uso de plantas medicinais. O trabalho foi realizado em duas etapas: (a a primeira foi a aplicação de um questionário de etnobotânica sobre dados socioeconômicos, conhecimentos e uso sobre plantas medicinais que foram entregues aos alunos para que entrevistassem pessoas da comunidade a qual pertenciam; (b a segunda etapa foi o desenvolvimento de atividades práticas sobre conceitos relacionados ao uso de planta medicinais. O estudo revelou que o conhecimento sobre o uso de plantas medicinais é praticado pelos moradores, principalmente pelas mulheres (70% e alunos que residem na região rural (50%. Os bairros que compõem a região Norte vêm sofrendo descaracterização da cultura da região fator atribuído  a implantação de casas populares, fato que pode ser notado no comportamento dos alunos e mudanças nos hábitos de cultivo e uso de plantas medicinais.

  4. Robo-AO M Dwarf Multiplicity Survey

    Science.gov (United States)

    Lamman, Claire; Baranec, Christoph; Berta-Thompson, Zachory K.; Law, Nicholas M.; Ziegler, Carl; Schonhut-Stasik, Jessica

    2018-06-01

    We analyzed close to 7,000 observations from Robo-AO’s field M dwarf survey taken on the 2.1m Kitt Peak telescope. Results will help determine the total multiplicity fraction and multiplicity functions of M dwarfs, which are crucial steps towards understanding their evolution and formation mechanics. Through its robotic, laser-guided, and automated system, the Robo-AO instrument has yielded the largest adaptive-optics M dwarf survey to date. I developed a graphical user interface to quickly analyze this data. Initial data analysis included assessing data quality, checking the result from Robo-AO’s automatic reduction pipeline, and determining existence as well as the relative position of companions through a visual inspection. This program can be applied to other datasets and was successfully tested by re-analyzing observations from a separate Robo-AO survey. After a conservative initial cut for quality, over 350 companions were found within 4” of a primary star out of 2,746 high quality Robo-AO M dwarf observations, including four triple systems. Further observations were done with the Keck II telescope by using its NIRC2 imager to follow up on ten select targets for the existence and physical association of companions. Future research will yield insights into low-mass stellar formation and provide a database of nearby M dwarf multiples that will potentially assist ongoing and future surveys for planets around these stars, such as the NASA TESS mission.

  5. Customer satisfaction and response to AOS

    Science.gov (United States)

    1999-01-01

    Passenger surveys were conducted in each of three successive springs in order to track passenger perception of changes in service quality during AOS implementation. In general, no improvement in passenger perceptions was observed relative to a high b...

  6. As LER/DORT no contexto do encontro simbólico entre pacientes e médicos peritos do INSS/SP

    Directory of Open Access Journals (Sweden)

    Anna Carolina Arena Siqueira

    2013-09-01

    Full Text Available Este artigo discute aspectos da relação médico-paciente no contexto do encontro simbólico entre portadores de LER/DORT e médicos peritos do Instituto Nacional de Seguro Social de São Paulo (INSS/SP. A pesquisa empírica que serviu de base à discussão foi de abordagem qualitativa e utilizou como instrumento para obtenção dos dados a entrevista em profundidade, com roteiros distintos para médicos peritos e pacientes. Foram realizadas 9 entrevistas, 6 com pacientes e 3 com médicos peritos. Análise de conteúdo foi aplicada ao material empírico segundo a referência conceitual das representações sociais. As narrativas dos portadores as LER/DORT, as narrativas dos médicos sobre o trabalho pericial e as narrativas de ambos acerca das consultas periciais possibilitaram explorar o encontro simbólico, não necessariamente factual, da consulta pericial. A relação médico-paciente que se estabelece nesse encontro simbólico é extremamente peculiar, pois está direcionada à avaliação do processo saúde-doença do paciente, com base em premissas do direito previdenciário, fator que descaracteriza a relação médico-paciente assentada no imaginário social como espaço de cuidado e confiança. Como consequência, portadores de LER/DORT e peritos, no momento do encontro, estão em diferentes escalas de poder: aqueles são avaliados e estes avaliam a validade da queixa segundo os padrões do INSS/SP. Por isso, apresentam narrativas sobre as consultas permeadas pela tensão relativa às expectativas e necessidades quanto aos resultados.

  7. Freshwater Bryozoa (Phylactolaemata) from Curaçao, Aruba and Bonaire

    NARCIS (Netherlands)

    Lacourt, A.W.

    1955-01-01

    The Phylactolaemata-fauna of Curaçao, Aruba and Bonaire, as it appears from the samples collected by dr P. Wagenaar Hummelinck, is connected with the fauna of the South-American mainland. The material belongs to three species only: Hyalinella agilis — Curaçao, Aruba, Bonaire — in temporary or

  8. Effect of garlic solution to Bacillus sp. removal

    Science.gov (United States)

    Zainol, N.; Rahim, S. R.

    2018-04-01

    Biofilm is a microbial derived sessile community characterized by cells that are irreversibly attached to a substratum or interface to each other, embedded in a matrix of extracellular polymeric substances that they have produced. Bacillus sp. was used as biofilm model in this study. The purpose of this study is to determine the effect of Garlic solution in term of ratio of water and Garlic solution (W/G) and ratio of Garlic solution to Bacillus sp. (GS/B) on Bacillus sp removal. Garlic solution was used to remove Bacillus sp. In this study, Garlic solution was prepared by crushing the garlic and mixed it with water. the Garlic solution was added into Bacillus sp. mixture and mixed well. The mixture then was spread on nutrient agar. The Bacillus sp. weight on agar plate was measured by using dry weight measurement method. In this study, initially Garlic solution volume and Garlic solution concentration were studied using one factor at time (OFAT). Later two-level-factorial analysis was done to determine the most contributing factor in Bacillus sp. removal. Design Expert software (Version 7) was used to construct experimental table where all the factors were randomized. Bacilus sp removal was ranging between 42.13% to 99.6%. The analysis of the results showed that at W/G of 1:1, Bacillus sp. removal increased when more Garlic solution was added to Bacillus sp. Effect of Garlic solution to Bacillus sp. will be understood which in turn may be beneficial for the industrial purpose.

  9. High-Performance CCSDS AOS Protocol Implementation in FPGA

    Science.gov (United States)

    Clare, Loren P.; Torgerson, Jordan L.; Pang, Jackson

    2010-01-01

    The Consultative Committee for Space Data Systems (CCSDS) Advanced Orbiting Systems (AOS) space data link protocol provides a framing layer between channel coding such as LDPC (low-density parity-check) and higher-layer link multiplexing protocols such as CCSDS Encapsulation Service, which is described in the following article. Recent advancement in RF modem technology has allowed multi-megabit transmission over space links. With this increase in data rate, the CCSDS AOS protocol implementation needs to be optimized to both reduce energy consumption and operate at a high rate.

  10. African Journals Online: Curaçao

    African Journals Online (AJOL)

    ... Cambodia, Cameroon, Canada, Cayman Islands, Central African Republic, Chad, Chile, China, Colombia, Comoros, Congo, Democratic Republic, Congo, Republic, Costa Rica, Côte d'Ivoire, Croatia, Cuba, Curaçao, Cyprus, Czech Republic, Denmark, Djibouti, Dominica, Dominican Republic, Ecuador, Egypt, Arab Rep.

  11. Streptomyces sp. Sebagai Biofungisida Patogen Fusarium oxysporum (Schlecht. f.sp. lycopersici (Sacc. Snyd. et Hans. Penyebab Penyakit Layu Pada Tanaman Tomat (Solanum lycopersicum L.

    Directory of Open Access Journals (Sweden)

    NURI MANDAN SARI

    2014-01-01

    Full Text Available A research was conducted to isolate Streptomyces sp. of soil Udayana University campus in theBukit-Jimbaran, to obtain the most effective Streptomyces sp. which is effective in inhibit the growth ofFusarium oxysporum f.sp. lycopersici, and to test response of tomato plants with Streptomyces sp.culture against Fusarium wilt desease. Implementation phases of the research consisted of isolation andidentification of Streptomyces sp, test the inhibition against F. oxysporum f.sp. lycopersici, and in vivotest used by dyeing the roots of the tomato plant (Solanum lycopersicum with Fusarium spores andafter 30 seconds the roots were dyeing Streptomyces culture. Furthermore, sterile soil in polybagwatered by Fusarium spores and Streptomyces culture at the same time. Based on morphologicalcharacteristic it found five isolates of Streptomyces sp.. The antagonist test showed Streptomyces sp.1 had ability (75% against Fusarium, Streptomyces sp 2 (68,3%, Streptomyces sp. 3 (71,6%,Streptomyces sp. 4 (63,3%, and Streptomyces sp. 5 (21,6%. All Streptomyces suppressed thegrowth of Fusarium on tomato plants in glass house (p<0,05. Streptomyces sp.3 suppressed Fusariumwilt disease in tomato from 88% in control to 20%.

  12. Crystallization and preliminary X-ray study of a family 10 alkali-thermostable xylanase from alkalophilic Bacillus sp. strain NG-27

    Energy Technology Data Exchange (ETDEWEB)

    Manikandan, K. [Department of Physics, Indian Institute of Science, Bangalore 560 012 (India); Bhardwaj, Amit [International Centre for Genetic Engineering and Biotechnology, New Delhi 110 067 (India); Ghosh, Amit [Institute of Microbial Technology, Sector 39-A, Chandigarh 160 036 (India); Reddy, V. S. [International Centre for Genetic Engineering and Biotechnology, New Delhi 110 067 (India); Ramakumar, S., E-mail: ramak@physics.iisc.ernet.in [Department of Physics, Indian Institute of Science, Bangalore 560 012 (India); Bioinformatics Centre, Indian Institute of Science, Bangalore 560 012 (India)

    2005-08-01

    A family 10 alkali-thermostable xylanase from Bacillus sp. NG-27 has been crystallized. A diffraction data set has been collected to 2.2 Å resolution. Xylanases (EC 3.2.1.8) catalyze the hydrolysis of β-1,4-glycosidic linkages within xylan, a major hemicellulose component in the biosphere. The extracellular endoxylanase (XylnA) from the alkalophilic Bacillus sp. strain NG-27 belongs to family 10 of the glycoside hydrolases. It is active at 343 K and pH 8.4. Moreover, it has attractive features from the point of view of utilization in the paper pulp, animal feed and baking industries since it is an alkali-thermostable protein. In this study, XylnA was purified from the native host source and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 174.5, b = 54.7, c = 131.5 Å, β = 131.2°, and diffract to better than 2.2 Å resolution.

  13. Crystallization and preliminary X-ray study of a family 10 alkali-thermostable xylanase from alkalophilic Bacillus sp. strain NG-27

    International Nuclear Information System (INIS)

    Manikandan, K.; Bhardwaj, Amit; Ghosh, Amit; Reddy, V. S.; Ramakumar, S.

    2005-01-01

    A family 10 alkali-thermostable xylanase from Bacillus sp. NG-27 has been crystallized. A diffraction data set has been collected to 2.2 Å resolution. Xylanases (EC 3.2.1.8) catalyze the hydrolysis of β-1,4-glycosidic linkages within xylan, a major hemicellulose component in the biosphere. The extracellular endoxylanase (XylnA) from the alkalophilic Bacillus sp. strain NG-27 belongs to family 10 of the glycoside hydrolases. It is active at 343 K and pH 8.4. Moreover, it has attractive features from the point of view of utilization in the paper pulp, animal feed and baking industries since it is an alkali-thermostable protein. In this study, XylnA was purified from the native host source and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 174.5, b = 54.7, c = 131.5 Å, β = 131.2°, and diffract to better than 2.2 Å resolution

  14. Elevated expression and potential roles of human Sp5, a member of Sp transcription factor family, in human cancers

    International Nuclear Information System (INIS)

    Chen Yongxin; Guo Yingqiu; Ge Xijin; Itoh, Hirotaka; Watanabe, Akira; Fujiwara, Takeshi; Kodama, Tatsuhiko; Aburatani, Hiroyuki

    2006-01-01

    In this report, we describe the expression and function of human Sp5, a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Our experiments employing Gal4-Sp5 fusion proteins reveal multiple transcriptional domains, including a N-terminal activity domain, an intrinsic repressive element, and a C-terminal synergistic domain. Elevated expression of Sp5 was noted in several human tumors including hepatocellular carcinoma, gastric cancer, and colon cancer. To study the effects of the Sp5 protein on growth properties of human cancer cells and facilitate the identification of its downstream genes, we combined an inducible gene expression system with microarray analysis to screen for its transcriptional targets. Transfer of Sp5 into MCF-7 cells that expressed no detectable endogenous Sp5 protein elicited significant growth promotion activity. Several of the constitutively deregulated genes have been associated with tumorigenesis (CDC25C, CEACAM6, TMPRSS2, XBP1, MYBL1, ABHD2, and CXCL12) and Wnt/β-Catenin signaling pathways (BAMBI, SIX1, IGFBP5, AES, and p21 WAF1 ). This information could be utilized for further mechanistic research and for devising optimized therapeutic strategies against human cancers

  15. Tolerância ao alumínio em porta-enxertos somaclonais de macieira cultivados em solução nutritiva

    Directory of Open Access Journals (Sweden)

    Dantas Adriana Cibele de Mesquita

    2001-01-01

    Full Text Available A macieira (Malus sp. cultivada em solos ácidos com pH inferior a 5,0 pode apresentar distúrbios nutricionais e inibição de crescimento causados por níveis tóxicos de Al que podem afetar a absorção do Ca, o qual apresenta estreita correlação com a produção de frutos de melhor qualidade. O objetivo deste trabalho foi verificar a tolerância ao Al em 18 somaclones e três cultivares porta-enxertos provenientes da seleção in vitro. As plântulas foram cultivadas durante 45 dias em copos de plástico de 300 mL contendo solução nutritiva e utilizando-se sílica lavada como substrato. Os valores obtidos foram submetidos à análise multivariada pelos componentes principais e agrupamento hierárquico baseado no método de Ward, o qual revelou ser mais adequado na classificação dos clones quanto à tolerância ao Al do que os testes de médias. O porcentual de redução na matéria seca da parte aérea e no número de folhas foram características que melhor possibilitaram a discriminação dos clones. Os clones foram classificados em três grupos: bem sensível ou não tolerante, moderadamente tolerante e tolerante.

  16. Fiscalização de verduras comercializadas no município de Ribeirão Preto, SP Monitoring of vegetables commercially sold in Ribeirão Preto, SP, Brazil

    Directory of Open Access Journals (Sweden)

    Osvaldo M. Takayanagui

    2001-02-01

    Full Text Available O consumo de verduras cruas constitui importante meio de transmissão de várias doenças infecciosas. Este estudo tem como objetivo a avaliação microbiológica e parasitológica de verduras comercializadas no município de Ribeirão Preto, SP, abrangendo todos os pontos de venda ao consumidor. Do total de 172 estabelecimentos fixos ou ambulantes analisados, 115 (67% apresentaram hortaliças com irregularidades: elevada concentração de coliformes fecais em 63%, presença de Salmonella em 9% e de enteroparasitas em 33%. Os pontos de venda com maior freqüência de hortaliças com resultados inadequados foram: mercearias (92%, CEAGESP (75%, quitandas (71%, vendedores ambulantes (71%, feiras-livres (69%, supermercados (52% e hortas (18%. O tipo de contaminação apresentou distribuição uniforme em relação aos locais de venda e à variedade da hortaliça. A maioria (61% das verduras contaminadas era procedente de hortas localizadas no município de Ribeirão Preto. Considerando a elevada freqüência de contaminação fecal e o potencial risco de doenças veiculadas pelas hortaliças, sugerimos uma vigilância sanitária mais atuante na fiscalização de alimentos oferecidos à população.The ingestion of raw vegetables represents an important means of transmission of several infectious diseases. The objective of the present study was to perform a microbiological and parasitological evaluation of the vegetables commercially sold in the municipality of Ribeirão Preto, SP, Brazil. Of a total of 172 commercial concerns analyzed, 115 (67% presented irregularities in the vegetables they sold, such as elevated concentration of fecal coliforms in 63%, presence of Salmonella in 9%, and presence of enteroparasites in 33%. The commercial concerns with the highest frequencies of vegetables showing inadequate results were: grocery stores (92%, CEAGESP (75%, fruit and vegetables stores (71%, traveling vendors (71%, fairs (69%, supermarkets (52%, and

  17. Is the lag screw sliding effective in the intramedullary nailing in A1 and A2 AO-OTA intertrochanteric fractures? A prospective study of Sliding and None-sliding lag screw in Gamma-III nail

    Directory of Open Access Journals (Sweden)

    Zhu Yi

    2012-09-01

    Full Text Available Abstract Object To compare the Sliding with Non-sliding lag screw of a gamma nail in the treatment of A1 and A2 AO-OTA intertrochanteric fractures. Materials and methods 80 patients were prospectively collected. In each group, AO/OTA 31-A were classified into group A. AO/OTA 31-A2.1 was classified as group B. We classified the A2.2 and A2.3 as group C. According to the set-screw locking formation of Gamma-III, the cases were randomly allocated to Sliding subgroup and Non-sliding subgroup in A, B and C groups. Follow-ups were performed 1, 3, 6 and 12 months postoperatively. Results In the Sliding group, the bone healing rate 3, 6, 12 months postoperatively reached 85.00%, 97.50%, 100% in group A, B and C. Meanwhile, in Non-sliding group, postoperatively, bone healing rate were 90.00%, 95.00% and 97.50% in group A, B and C, respectively. Both differences were not significant. Lower limb discrepancy between Sliding and Non-sliding pattern was significantly different in group C which represent fracture types of AO/OTA 31-A2.2 and A2.3 (0.573 ± 0.019 mm in Non-sliding group, 0.955 mm ± 0.024 mm in Sliding group, P Conclusions As a result, we can conclude that the sliding distance is minimal in Gamma nails and it is related to the comminuted extent of the intertrochanteric area in A1 and A2 AO-OTA intertrochanteric fractures. For treating these kinds of fractures, the sliding of the lag screw of an Gamma nail does not improve any clinical results and in certain cases, such as highly comminuted A1 and A2 fractures, can therefore even benefit from a locked lag screw by tightening the set-screw.

  18. Anti-Helicobacter pylori metabolites from Rhizoctonia sp. Cy064, an endophytic fungus in Cynodon dactylon.

    Science.gov (United States)

    Ma, Y M; Li, Y; Liu, J Y; Song, Y C; Tan, R X

    2004-07-01

    A new benzophenone, named rhizoctonic acid (1), together with three known compounds monomethylsulochrin (2), ergosterol (3) and 3beta,5alpha,6beta-trihydroxyergosta-7,22-diene (4) were isolated through bioassay-guided fractionations from the culture of Rhizoctonia sp. (Cy064), an endophytic fungus in the leaf of Cynodon dactylon. The structure of the new acid 1 was elucidated to be 5-hydroxy-2-(2-hydroxy-6-methoxy-4-methylbenzoyl)-3-methoxybenzoic acid by a combination of spectral analyses. Furthermore, the structure of monomethylsulochrin 2 was confirmed by 13C-NMR analysis. All four metabolites were subjected to a more detailed in vitro assessment of their antibacterial action against five clinically isolated and one reference (ATCC 43504) Helicobacter pylori strains.

  19. Wnt-mediated down-regulation of Sp1 target genes by a transcriptional repressor Sp5

    Czech Academy of Sciences Publication Activity Database

    Fujimura, Naoko; Vacík, Tomáš; Machoň, Ondřej; Vlček, Čestmír; Scalabrin, S.; Speth, M.; Diep, D.; Krauss, S.; Kozmik, Zbyněk

    2007-01-01

    Roč. 282, č. 2 (2007), s. 1225-1237 ISSN 0021-9258 Institutional research plan: CEZ:AV0Z50520514 Keywords : Wnt -mediated signaling * Sp5 transcription factor * Sp1 target genes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.581, year: 2007

  20. O direito fundamental ao conteúdo do próprio trabalho: uma reconstrução normativa do direito ao trabalho

    OpenAIRE

    Wandelli, Leonardo Vieira

    2016-01-01

    Resumo: Pretende-se enfrentar a inefetividade do direito humano e fundamental ao trabalho a partir de um esforço de reconstrução normativa da sua fundamentação, apoiada em um duplo pilar teórico, nas teorias das necessidades e nas teorias do reconhecimento. Com isso, não somente se recupera a importância do vínculo entre trabalho e dignidade humana, como se explicitam aspectos essenciais da centralidade do trabalho para os sujeitos. A partir disso, o direito ao trabalho se desvela não apenas ...

  1. The Role of S P2, SP3 AND SP4 in The Transcriptional Regulation of The Promoter of Nuclear Encoded Mitochondrial Genes

    International Nuclear Information System (INIS)

    Zaid, A.; Salem, Gh.

    2012-01-01

    The GC-box is an important transcriptional regulatory element present in the promoters of many mammalian genes, and is found in most, if not all, oxidative phosphorylation (OXPHOS) promoters. In the present study we examine the effects of three Spl family members (Sp2, Sp3, and Sp4) on the adenine nucleotide translocase 2, cytochrome cl, Fl-ATPase β-subunit, and the mitochondria transcription factor (mtTFA) promoters in Drosophila SL2 cell line. Sp3, like Spl, strongly activates transcription all four promoters. SP4 stimulates, moderately, but Sp2 had no effect. In addition, Sp3 can, like Spl, inhibit transcription from the proximal promoter of the ANT2 gene through binding to the Cbox GC element. By contrast, Sp4 and Sp2 do not repress promoter activity. Furthermore, since Sp4 and Sp2 bind to the Cbox repression element on the ANT2 promoter, but do not repress transcription, inhibition of transcription cannot be explained by steric hindrance of pre-initiation complex assembly. These data suggest that different Spl family members differentially affect transcription from the OXPHOS promoters.

  2. Evaluation of antioxidant and cytotoxic properties of Cynobacteria, Limnothrix sp. and Leptolyngbya sp. from Arabian sea

    Digital Repository Service at National Institute of Oceanography (India)

    Anas, A.; Vinothkumar, S.; Gupta, S.; Jasmin, C.; Joseph, V.; Parameswaran, P.S.; Nair, S.

    The hexane fractions of the marine cyanobacteria: Leptolyngbya sp. and Limnothrix sp., collected from Arabian Sea were found to display promising antioxidant properties than their ethyl acetate fraction during radical scavenging ABTS/DPPH assays (IC...

  3. REPRESENTAÇÃO GRÁFICA DOS ELEMENTOS DO CLIMA: ESTUDO COMPARATIVO ENTRE MARINGÁ, PR E BOTUCATU, SP

    Directory of Open Access Journals (Sweden)

    Emerson Galvani

    2006-12-01

    Full Text Available A representação gráfica de quaisquer variáveis apresenta uma dupla finalidade: a primeira relacionada à sua variação no tempo e no espaço, e a segunda ao seu caráter didático. Os elementos do clima, como temperatura do ar e do solo, umidade relativa do ar, radiação solar global (direta e difusa, saldo de radiação (onda curta e onda longa, direção e velocidade do vento, pressão atmosférica, índice de chuvas e demais componentes derivados destes, evidenciam ao longo dos dias, dos meses e dos anos uma determinada curva característica que representa as condições de tempo e clima de determinada região. O que diferencia essa ou aquela localidade em termos climáticos é a amplitude da variação desses elementos no tempo e no espaço. Por exemplo, a curva diária dos níveis de radiação solar global em superfície em duas distintas localidades, situadas em diferentes latitudes, é idêntica, e o que as diferencia é a amplitude dos valores máximos e a energia total integrada ao longo do dia e do ano. O objetivo do presente trabalho é apresentar curvas instantâneas dos principais elementos do clima mostrando sua variação instantânea, diária e sazonal para as condições climáticas de Botucatu,SP e Maringá,PR, Brasil.

  4. As sementes geneticamente modificadas e o direito ao desenvolvimento

    Directory of Open Access Journals (Sweden)

    Milena Barbosa Melo

    2017-12-01

    Full Text Available O presente artigo apresenta como objeto de investigação a ligação entre os direitos da propriedade intelectual no contexto dos organismos geneticamente modificados (OGM´S. Objetivou-se, especificamente, responder inquietações acerca dos danos reais para os consumidores, bem como o lucro para as empresas multinacionais, sem desconsiderar, ainda, os efetivos danos ao meio ambiente e à saúde. Utilizou metodologia pautada em pesquisa bibliográfica e documental a partir do método hermenêutico fazendo uso da interpretação judicial-histórica. Conclui pela relevância da temática para efetivação do direito ao desenvolvimento, bem como pela urgência de uma regulamentação da biossegurança, de forma a permitir melhor sustentação aos OGM´s.

  5. Study of fungicidal properties of colloidal silver nanoparticles (AgNPs on trout egg pathogen, Saprolegnia sp.

    Directory of Open Access Journals (Sweden)

    Seyed Ali Johari

    2015-05-01

    Full Text Available Silver nanoparticles (AgNPs are known to have bactericidal and fungicidal effects. Since, there is few information available on the interaction of colloidal nanosilver with fish pathogens. Hence, the current study investigated the effects of colloidal AgNPs on the in vitro growth of the fish pathogen Saprolegnia sp.. Before the experiments, various important properties of AgNPs were well-characterized. The antifungal activity of AgNPs was then evaluated by determining the minimum inhibitory concentrations (MICs using two-fold serial dilutions of colloidal nanosilver in a glucose yeast extract agar at 22ºC. The growth of Saprolegnia sp. on the AgNPs agar treatments was compared to that of nanosilver-free agar as controls. The results showed that AgNPs have an inhibitory effect on the in vitro growth of the tested fungi. The MIC of AgNPs for Saprolegnia sp. was calculated at 1800 mg/L, which is equal to 0.18 percent. It seems that AgNPs could be a proper replacement for teratogenic and toxic agents, such as malachite green. In addition, the indirect use of AgNPs could be a useful method for providing new antifungal activity in aquaculture systems.

  6. Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments.

    Science.gov (United States)

    Roca, Christophe; Lehmann, Mareen; Torres, Cristiana A V; Baptista, Sílvia; Gaudêncio, Susana P; Freitas, Filomena; Reis, Maria A M

    2016-06-25

    Exopolysaccharides (EPS) are polymers excreted by some microorganisms with interesting properties and used in many industrial applications. A new Pseudoalteromonas sp. strain, MD12-642, was isolated from marine sediments and cultivated in bioreactor in saline culture medium containing glucose as carbon source. Its ability to produce EPS under saline conditions was demonstrated reaching an EPS production of 4.4g/L within 17hours of cultivation, corresponding to a volumetric productivity of 0.25g/Lh, the highest value so far obtained for Pseudoalteromonas sp. strains. The compositional analysis of the EPS revealed the presence of galacturonic acid (41-42mol%), glucuronic acid (25-26mol%), rhamnose (16-22mol%) and glucosamine (12-16mol%) sugar residues. The polymer presents a high molecular weight (above 1000kDa). These results encourage the biotechnological exploitation of strain MD12-642 for the production of valuable EPS with unique composition, using saline by-products/wastes as feedstocks. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Resistências aos Antibióticos em Pneumologia

    Directory of Open Access Journals (Sweden)

    J.G. Saraiva da Cunha

    1999-01-01

    Full Text Available RESUMO: A crescente resistência dos microrganismos aos agentes antimicrobianos tem motivado o interesse da comunidade científica com o objectivo de avaliar a dimensão do problema, perceber a sua origem e encontrar eveotuais soluções. São quatro os mecanismos basicos de resistência das bácterias aos antibióticos: inactivação enzimática, alteração ou excesso de produção das molécules alvo, diminuição da permeabilidade da parede celulalr e eliminação actia (eflauxo do antibiótico para o exterior da bactéria. Para cada uma das diferentes classes de antibioticos sio referidos os priocipais mecanismos de resisteocia, assim como se exemplifica quais os germes de particular relevância em pneumologia que maiores dificuldades terapêuticas actualmente colocam. Finalmente enumeram-se algumas medidas para contrariar a expansão do problema. Palavras-Chave: Resistências aos antibióticos, Pneumologia, Pneumococo

  8. ENSAIO: HOMENAGEM AO PROFESSOR REINIER ROZESTRATEN

    Directory of Open Access Journals (Sweden)

    Antonio C. R. Tupinambá

    2014-02-01

    Full Text Available Trata-se de uma homenagem póstuma ao Professor Doutor Reinier Rozestraten. O texto apresenta, suscintamente, o percurso acadêmico do nomeado professor, em especial a sua passagem pela Psicologia do Trânsito. Paralelamente discorre sobre sua principal obra na área, que, de certo modo introduz o discurso psicológico sobre o comportamento de trânsito em perspectiva nacional, juntamente com sua preocupação em imprimir um caráter científico ao tema e introduzi-lo no contexto da academia e na sociedade em perspectivas teórica e prática. A obra escolhida é considerada um marco para o estabelecimento teórico da disciplina da psicologia do trânsito no Brasil. A homenagem foi originalmente escrita por ocasião dos cinco anos de falecimento do professor Reinier.

  9. Progress in SP-100 tribological coatings

    International Nuclear Information System (INIS)

    Ring, P.J.; Roy, P.; Schuster, G.B.; Busboom, H.J.

    1992-01-01

    The SP-100 reactor will operate at temperatures up to 1500K in high vacuum. To address the SP-100 needs, a tribology development program has been established at GE to investigate candidate coating materials. Materials were selected based on their high thermodynamic stability, high melting point, compatibility with the substrate, and coefficients of thermal expansion similar to niobium-1% zirconium-the candidate structural material for SP-100. An additional requirement was that the deposition processes should be commercially available to coat large components. This paper presents the details regarding the SP-100 Tribology Development Program including background information, specific bearing requirements, basis for coating material selection, testing methods and the initial results covering the early years of this program

  10. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

    Directory of Open Access Journals (Sweden)

    Samiha Sioud

    2009-01-01

    Full Text Available Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002, as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.

  11. Desempenho e retenção de nitrogênio de frangos de corte dos 22 aos 42 dias alimentados com rações com diferentes níveis de cálcio e suplementadas com Fitase Performance and nitrogen retention in broilers from 22 to 42 days old fed diets with different levels in calcium supplement with Fitase

    Directory of Open Access Journals (Sweden)

    Antônio Soares Teixeira

    2003-02-01

    Full Text Available Com o objetivo de avaliar os níveis de cálcio na ração suplementada com enzima fitase para frangos de corte de 22 a 42 dias de idade, foi conduzido um experimento com 600 frangos de 21 dias da linhagem Hubbard-MPK, distribuídos em um delineamento inteiramente casualizado, em arranjo fatorial cinco x dois (níveis de cálcio da dieta x sexos, com três repetições de 20 aves por parcela. A ração basal foi balanceada para atender às exigências nutricionais das aves, conforme o NRC (1994, exceto para o cálcio, cujos níveis foram 0,40; 0,59; 0,78; 0,97 e 1,16%, suplementada com Fitase. Aos 35 dias de idade, duas aves de cada tratamento foram transferidas para gaiolas de metabolismo para coleta total das excretas durante quatro dias. O consumo de ração e o ganho de peso foram de 12,8 e 16% respectivamente, e superiores (P0,05 para o consumo de ração, ganho de peso e conversão alimentar em razão dos níveis crescentes de cálcio nas rações. A digestibilidade da matéria seca foi melhor com o nível de 0,88% de cálcio, e a maior retenção de nitrogênio foi obtida com 0,70% de cálcio na ração suplementadas com fitase. Conclui-se que o aumento dos níveis de cálcio em rações suplementadas com fitase para frangos de corte dos 22 aos 42 dias de idade não afeta o desempenho dos mesmos, e níveis muito baixos de cálcio reduzem a digestibilidade da matéria seca e retenção de nitrogênio das rações.With the objective of evaluating the levels of calcium in the ration supplemented with phytase for broiler chickens from 22 to 42 days old, one experiment was conducted by using a total of 600 chickens of 22 days old Hubardb-MPK strain, distributed in a completely randomized design, in five two factorial arrangement (levels of calcium of the diet x sexes, with three replicates of 20 birds per plot. The basal diets was formulated to meet the nutritional requirements of the broilers according to NRC (1994, except for calcium, whose

  12. Talaroxanthone, a novel xanthone dimer from the endophytic fungus Talaromyces sp. associated with Duguetia stelechantha (Diels) R.E. Fries

    International Nuclear Information System (INIS)

    Koolen, Hector H.F.; Universidade Estadual de Campinas; Menezes, Lais S.; Souza, Mayane P.; Silva, Felipe M.A.; Almeida, Fabiana G.O.; Souza, Afonso D.L. de; Souza, Antonia Q.L. de; Nepel, Angelita; Barison, Andersson; Silva, Flavio Henrique da; Evangelista, Danilo Elton

    2013-01-01

    DgCr22.1b, an endophytic isolate of Talaromyces sp., was collected in the Amazonian Rain forest from the medicinal plant Duguetia stelechantha. From the fractionation of the methanolic mycelial extract, a new xanthone dimer talaroxanthone was isolated. The structure of this new compound was elucidated based on spectroscopic analyses including 2D nuclear magnetic resonance (NMR) experiments. (author)

  13. Talaroxanthone, a novel xanthone dimer from the endophytic fungus Talaromyces sp. associated with Duguetia stelechantha (Diels) R.E. Fries

    Energy Technology Data Exchange (ETDEWEB)

    Koolen, Hector H.F., E-mail: hectorkoolen@gmail.com [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Departamento de Quimica; Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Instituto de Quimica; Menezes, Lais S.; Souza, Mayane P.; Silva, Felipe M.A.; Almeida, Fabiana G.O.; Souza, Afonso D.L. de [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Departamento de Quimica; Souza, Antonia Q.L. de [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Escola Superior de Ciencias da Saude; Nepel, Angelita; Barison, Andersson [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Departamento de Quimica; Silva, Flavio Henrique da; Evangelista, Danilo Elton [Universidade Federal de Sao Carlos (UFSC), SP (Brazil). Departamento de Genetica e Evolucao

    2013-05-15

    DgCr22.1b, an endophytic isolate of Talaromyces sp., was collected in the Amazonian Rain forest from the medicinal plant Duguetia stelechantha. From the fractionation of the methanolic mycelial extract, a new xanthone dimer talaroxanthone was isolated. The structure of this new compound was elucidated based on spectroscopic analyses including 2D nuclear magnetic resonance (NMR) experiments. (author)

  14. O sindicalismo mineiro frente aos desafios dos anos 90

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    Antônio Carvalho Neto

    2000-12-01

    Full Text Available O que se analisa neste artigo é como os sindicatos dos bancários, metalúrgicos e trabalhadores em telecomunicações têm negociado o (desemprego, a demanda por qualificação e a remuneração variável. Foram tabuladas/analisadas 3.569 cláusulas de acordos coletivos, e realizadas 26 entrevistas com dirigentes sindicais e empresariais em três Estados (RJ, SP e MG. Os resultados indicam que as mudanças organizacionais foram incomparavelmente mais negociadas no setor metalúrgico do ABC. A flexibilização da jornada foi trocada pela redução (da jornada sem redução de salário nos setores metalúrgicos paulistas, mas não em Minas Gerais. No setor bancário, houve poucos resultados nas negociações sobre jornada. As negociações em torno da remuneração variável vinculada às metas das empresas aconteceram nos setores metalúrgicos, e não nos setores de serviços. As entrevistas apontam para um sindicalismo mais propositivo, no sentido de uma intervenção negociada, predisposição que não é correspondida pelos empresários, grosso modo. O Estado, que poderia utilizar seu poder regulador para induzir os empresários a negociarem nessa direção não tem produzido resultados satisfatórios. Conclui-se que não há maturidade empresarial no Brasil que permita ao Estado ausentar-se das relações de trabalho.

  15. Nanocrystalline sp{sup 2} and sp{sup 3} carbons: CVD synthesis and applications

    Energy Technology Data Exchange (ETDEWEB)

    Terranova, M. L. [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy); Rossi, M. [Università degli Studi di Roma “Sapienza,” via A. Scarpa, Dipartimento di Scienze di Base e Applicate per l’Ingegneria and Centro di Ricerca per le Nanotecnologie Applicate all’Ingegneria (CNIS) (Italy); Tamburri, E., E-mail: emanuela.tamburri@uniroma2.it [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy)

    2016-11-15

    The design and production of innovative materials based on nanocrystalline sp{sup 2}- and sp{sup 3}-coordinated carbons is presently a focus of the scientific community. We present a review of the nanostructures obtained in our labs using a series of synthetic routes, which make use of chemical vapor deposition (CVD) techniques for the selective production of non-planar graphitic nanostructures, nanocrystalline diamonds, and hybrid two-phase nanostructures.

  16. Molecular Characterization of Isolated Xanthomonas sp. From Rice Rhizosphere Soil and their Inhibition using Clerodendrum viscosum Vent

    International Nuclear Information System (INIS)

    Islam, R.; Ahmed, J.; Al-Reza, S. M.; Rahman, M. M.; Nyme, Z.; Lisa, L. A.; Yousaf, M.; Alam, M. N.

    2015-01-01

    In this study, we characterized some Xanthomonas sp. from rice rhizosphere soil by 16S rDNA sequence analysis and the potentiality tested of oil and extracts of Clerodendrum viscosum Vent. leaves against those isolated bacteria. The essential oil compositions were evaluated by GC-MS analysis and identified twenty four compounds as 94.37% of the total essential oil, where cyclohepta-1,3,5,-triene (16.07%), 2H-1,4-pyridoxazin-3(4H)-one (12.27%), L-carvenol (8.04%), p-cymene (9.15%), alpha-terpineol (7.98%), beta-sitosterol (6.76%), tyranton (6.42%), mesityl oxide (6.34%), gamma-terpinene (5.42%) and beta-linalool (3.74%) were the major constituents. According to the 16S rDNA sequences analysis, Hb28 and Xb43 isolates have shown the 100% similarity for Xanthomonas retroflexus and Xanthomonas theicola. In addition, isolates Ha18, Xb33 and Jb30 also have shown the maximum similarity of 98% for Xanthomonas sp. TB8-9-II, Xanthomonas campestris and Xanthomonas sp. BBCT38. Furthermore, in vitro studies showed that the oil have the inhibitory effect on Xanthomonas sp. TB8-9-II, X. campestris, X. retroflexus, Xanthomonas sp. BBCT38, and X. theicola, where the zone of inhibition and MIC values were 18.8 to 25.9 mm and 62.5 to 125 meu g ml/sup -1/, respectively. However, organic extracts showed the zone of inhibition and MIC values were 13.4 to 22.9 and 62.5 to 500 meu g ml-1, respectively. Our findings suggested that the essential oil and extracts of C. viscosum Vent. leaves might be the effective source to control Xanthomonas sp. biologically. (author)

  17. Levantamento de plantas aquáticas no reservatório de Salto Grande, Americana-SP Aquatic plant survey in Salto Grande reservoir in Americana-SP, Brazil

    Directory of Open Access Journals (Sweden)

    D. Martins

    2011-03-01

    Full Text Available O objetivo deste estudo foi determinar a frequência relativa e o nível de infestação de cada espécie da flora aquática presente no reservatório de Salto Grande, Americana-SP. O levantamento e a identificação das plantas aquáticas foram realizados percorrendo-se as margens do reservatório em uma embarcação. Ao longo dele foram estabelecidos 20 pontos de avaliação, sendo todos eles fotografados e georreferenciados. Foram atribuídos valores de 0 a 100% tanto para as espécies presentes como para os espaços livres de macrófitas aquáticas que eventualmente pudessem ocorrer dentro dos pontos amostrados. Com os dados referentes ao número de indivíduos e pontos avaliados, foi determinada a frequência relativa de cada espécie. Foram identificadas 13 espécies em todo o reservatório, sendo 12 vasculares e uma de alga-verde (Chlorella spp.. Entre as espécies vasculares, nove eram plantas emersas flutuantes, as quais poderiam estar ou não ancoradas no leito do reservatório: Alternanthera philoxeroides, Brachiaria subquadripara, Cyperus difformis, Echinochloa polystachia var. spectabilis, Eichhornia crassipes, Panicum rivulare, Pistia stratiotes, Salvinia auriculata e Typha angustifolia. Outras três espécies foram encontradas somente em solo firme alagado: Aeschynomene sensitiva, Hedychium coronarium e Mimosa pigra.The objectives of this work were to determine the relative frequency and infestation level of each species present in the Salto Grande Reservoir in Americana-SP. The survey and identification of the aquatic plants were carried out in the Salto Grande reservoir on a boat. Twenty evaluation sites were established along the reservoir, photographed and geo-referenced. Values from 0 to 100% were assigned for both the species present and for the aquatic plants that could possibly occur within the sampled sites. Based on the number of individuals and points evaluated, the relative frequency of each species was determined

  18. Three New Indole Diterpenoids from the Sea-Anemone-Derived Fungus Penicillium sp. AS-79.

    Science.gov (United States)

    Hu, Xue-Yi; Meng, Ling-Hong; Li, Xin; Yang, Sui-Qun; Li, Xiao-Ming; Wang, Bin-Gui

    2017-05-12

    Three new indolediterpenoids, namely, 22-hydroxylshearinine F ( 1 ), 6-hydroxylpaspalinine ( 2 ), and 7- O -acetylemindole SB ( 3 ), along with eight related known analogs ( 4 - 11 ), were isolated from the sea-anemone-derived fungus Penicillium sp. AS-79. The structures and relative configurations of these compounds were determined by a detailed interpretation of the spectroscopic data, and their absolute configurations were determined by ECD calculations ( 1 and 2 ) and single-crystal X-ray diffraction ( 3 ). Some of these compounds exhibited prominent activity against aquatic and human pathogenic microbes.

  19. SIMS chemical and isotopic analysis of impact features from LDEF experiments AO187-1 and AO187-2

    Science.gov (United States)

    Stadermann, Frank J.; Amari, Sachiko; Foote, John; Swan, Pat; Walker, Robert M.; Zinner, Ernst

    1995-01-01

    Previous secondary ion mass spectrometry (SIMS) studies of extended impact features from LDEF capture cell experiment AO187-2 showed that it is possible to distinguish natural and man-made particle impacts based on the chemical composition of projectile residues. The same measurement technique has now been applied to specially prepared gold target impacts from experiment AO187-1 in order to identify the origins of projectiles that left deposits too thin to be analyzed by conventional energy-dispersive x-ray (EDX) spectroscopy. The results indicate that SIMS may be the method of choice for the analysis of impact deposits on a variety of sample surfaces. SIMS was also used to determine the isotopic compositions of impact residues from several natural projectiles. Within the precision of the measurements all analyzed residues show isotopically normal compositions.

  20. Ocorrência de Fusarium solani f. sp. piperis em Piper nigrum no estado de Alagoas Report of Fusariom solani f. sp. piperis in Piper nigrum in the state of Alagoas

    Directory of Open Access Journals (Sweden)

    Juliana Paiva Carnaúba

    2007-03-01

    Full Text Available A pimenta-do-reino (Piper nigrum L. é uma planta trepadeira, pertencente à família Piperaceae. Ela é originária do Sudeste Asiático, sendo a mais comum e importante das especiarias. A fusariose, também conhecida por podridão do pé e podridão das raízes é a principal doença da cultura, de ocorrência restrita ao Brasil. Um isolado de Fusarium sp., encontrado infectando plantas de pimenta-do-reino cv. bragantina no município de União dos Palmares em Alagoas, foi caracterizado morfologicamente e teve sua patogenicidade confirmada em mudas deste hospedeiro. Os macroconídios apresentaram-se falcados, hialinos com três a cinco septos, com dimensões de 30,5 - 26,5 x 6,3 - 4,9 ìm, enquanto os microconídios apresentaram-se hialinos, unicelulares, elípticos ou alantóides medindo 16,6 - 4,9 x 6,5 - 3,3 ìm. Os clamidósporos foram abundantes em meio batata-dextrose-ágar. O isolado foi identificado como Fusarium solani f. sp. piperis Alb. tratando-se do primeiro relato deste patógeno em pimenta-do-reino no estado de Alagoas.The pepper-of the-kingdom (Piper nigrum L. it is a climbing plant, pertaining plant to the Piperaceae family. It is originary the southeastern Asian, being most common and important of the spices. Seedling death, also known for rottenness of the foot and rottenness of the root is the main illness of the culture, restricted occurrence to Brazil. Isolated of Fusarium sp., found contamined plants of bragantina pepper-do-kingdom cv. in the city of União dos Palmares in Alagoas, was characterized morphologycament and had its pathogenicity confirmed in changes of this host. The macroconidia slightly curved, typically canoe-shaped, hyaline with three the five septate, measuring 30,5 - 26,5 x 6,3 - 4,9 ìm. Microconidia hyaline, unicellulars, ellipticals or allantoises measuring 16,6 - 4,9 x 6,5 - 3,3 ìm. The chlamydospores had been abundant in half potato-dextrose-agar. The isolated one was identified as to Fusarium

  1. Holocene Evolution of Qing'ao Embayment, Southern China

    Science.gov (United States)

    Switzer, A. D.; Yu, F.; Chen, B.; Zheng, Z.; Wang, D.

    2012-12-01

    The Holocene evolution of the Qing'ao embayment, Nan'ao Island, southern China, is primarily the result of the interaction of tectonic activity, climate variation and changes in relative sea level. Characterizing the evolutionary history of the relatively small Qing'ao embayment during the Holocene will help improve our understanding of the driving mechanisms of coastal evolution in the area. To reconstruct the Holocene evolution history we analyzed the grain size, loss on ignition (LOI) and carbonate content of modern and core samples. Modern environmental analogs were examined in surface samples ranging from the coastal sand dunes through to offshore. The results of these modern samples suggest that dune sand (mean size of ~2.33Phi) are slightly finer than beach sand (mean size of 2.13Phi), and nearshore sediment is much coarser than offshore sediment (mean size of 5.90Phi). This modern analogs were then applied to 8 percussion cores from the Qing'ao embayment. A chronological framework obtained from 11 radiocarbon samples suggests that the embayment started to accept deposition since early Holocene, ~8500 cal. yr. BP. Three main phases of Holocene evolution were identified. A basin wide shell-rich sand sheet forms the basal Holocene facies and overlies clay rich presumably Pleistocene sediments or bedrock. This facies records an initial sedimentation phase associated with the early Holocene transgression into the embayment (~8500-6000 cal. yr. BP). The basal facies grades upward to a mixed sandy-mud facies which includes lagoonal clayey-silts, flood tide delta sands and records an estuarine phase lasting from ~6000-1000 cal. yr. BP that appears coincident with falling regional sea levels. Coincident with the estuarine phase is a period of coastal dune building recorded as yet undated massive sands that are found in the upper fill. Toward the end of the estuarine phase it is apparent that dune migration has restricted the lagoon entrance and that this was

  2. Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae.

    Science.gov (United States)

    Liu, Qian-Qian; Li, Xiao-Li; Rooney, Alejandro P; Du, Zong-Jun; Chen, Guan-Jun

    2014-10-01

    A novel Gram-stain-negative, facultatively anaerobic, catalase- and oxidase-positive, non-motile and pink-pigmented bacterium, designated G22(T), was isolated from Gahai, a saltwater lake in Qinghai province, China. Optimal growth occurred at 33-35 °C, pH 7.0-7.5, and in the presence of 2-4% (w/v) NaCl. The DNA G+C content was 40.0 mol%. The major polar lipids were phosphatidylethanolamine and three unknown lipids. The predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and iso-C15:0 3-OH, and MK-7 was the main respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain G22(T) fell within the class Bacteroidia. Its closest phylogenetic neighbour was the recently described species Draconibacterium orientale, the sole member of the family Draconibacteriaceae, with merely 90.04% sequence similarity. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence observed, a novel species in a new genus, Tangfeifania diversioriginum gen. nov., sp. nov., is proposed within the family Draconibacteriaceae. The type strain is G22(T) ( = CICC 10587(T) =DSM 27063(T)).

  3. Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics Translocação de Klebsiella sp. em camundongos alimentados com dieta enteral contendo prebióticos

    Directory of Open Access Journals (Sweden)

    Daniele Ferreira da Silva

    2009-04-01

    tratamentos IV, V, VIII e IX foram alimentados com dieta enteral contaminada com, aproximadamente, 10(10UFC/g de Klebsiella pneumoniae. Em tempos definidos (sexto, sétimo, nono, décimo primeiro e décimo terceiro dia de experimento, dois animais de cada grupo foram sacrificados e seus órgãos: baço, coração, fígado, pulmões e rins, foram coletados assepticamente, pesados e analisados quanto à presença de colônias típicas de Klebsiella sp. RESULTADOS: Maior número de UFC/g de Klebsiella sp. foi detectado em órgãos de animais do grupo imunodeprimidos, que receberam dieta com Klebsiella pneumoniae e sem prebióticos. Animais que receberam dieta enriquecida com frutooligossacarídeo e inulina, na concentração de 15,3mg/g de peso corporal, apresentaram menor período para a translocação de Klebsiella sp., quando comparados aos animais que não receberam prebióticos na dieta. CONCLUSÃO: A adição de frutooligossacarídeos e inulina em dietas enterais na dose de 15,3mg/g de peso corporal resultou na diminuição de translocação de Klebsiella para baço, coração, fígado, pulmão e rins de camundongos que receberam a dieta contaminada associada ou não com droga imunodepressora e antibiótico.

  4. AS DINÂMICAS DA RELAÇÃO ENTRE COMÉRCIO E INDÚSTRIA EM FRANCA (SP

    Directory of Open Access Journals (Sweden)

    Judite de Azevedo do Carmo

    2016-08-01

    Full Text Available Objetiva-se, neste texto, a apresentação das dinâmicas da relação estabelecida entre comércio e indústria, na contemporaneidade, destacando as questões teóricas inerentes ao tema e o exemplo empírico do Arranjo Produtivo Local de Franca (SP especializado na produção de calçados masculinos. Para tanto recorreu-se à pesquisa bibliográfica para embasamento da reflexão teórica, e tomou-se como base para a análise empírica, os dados e informações levantados por Carmo (2012 em órgãos institucionais, como o Instituto de Estudos e Marketing Industrial (IEMI, o Sindicato das Indústrias de Calçados de Franca (Sindifranca, bem como por intermédio de entrevistas e questionários aplicados aos proprietários de estabelecimentos comercias e de indústrias locais. Os dados obtidos junto ao IEMI serviram para a análise quantitativa, em nível de APL, enquanto aqueles obtidos por meio de questionários, aplicados a 12 indústrias locais, foram a base da análise qualitativa, em nível de indústria. A análise aí proposta permitiu o entendimento de que as transformações ocorridas no processo produtivo, nas décadas finais do século XX, condicionaram o estreitamento das relações entre a indústria e o comércio, sendo que ambas as atividades são realizadas em dependência mútua e que no APL, em destaque, há pouca relação entre as indústrias e o comércio local, havendo um monopólio das primeiras sobre a comercialização de seus produtos.

  5. Aspectos morfológicos dos órgãos genitais femininos do gambá (Didelphis sp.

    Directory of Open Access Journals (Sweden)

    Natalia Nardelli Gonçalves

    2009-08-01

    Full Text Available Foram coletados oito sistemas reprodutores de fêmeas de gambás (Didelphis sp. Destes cinco, foram destinados ao estudo macroscópico e três ao estudo microscópico. Estes animais encontravam-se fixados no Departamento de Anatomia da UNIfeob, em formaldeído a 10%, provenientes do Criatório Científico de Araçatuba (IBAMA. Para análise macroscópica, os sistemas reprodutores foram retirados, guardando-se as devidas posições "in situ" e foi realizada a documentação fotográfica para resultados macroscópicos. Para o estudo microscópico, o sistema reprodutor foi processado rotineiramente pela técnica de inclusão em Paraplast e cortadas em micrótomo, com espessura média de 5 mm, e os cortes foram corados em HE, Azul de Toluidina, Picrosírius, PAS e tricromo de Masson16,17 . O sistema reprodutor feminino do gambá era composto por dois ovários, útero duplo, duas vaginas laterais e entre estas um canal pseudovaginal, estas três estruturas se unem formando o seio urogenital encerrando em um "monte" púbico ou tubérculo genital, formando uma abertura comum interna ao ânus e a vulva, chamada de pseudocloaca ou seio urogenital. Microscopicamente os ovários eram funcionais com folículos mono e poliovulares. A tuba uterina era característica, revestida por epitélio prismático ciliado. Os cornos uterinos apresentaram epitélio de revestimento prismático ciliado. O seio urogenital apresentou três estruturas tubulares. A vulva apresentou as mesmas características da pele.

  6. Paenibacillus oenotherae sp. nov. and Paenibacillus hemerocallicola sp. nov., isolated from the roots of herbaceous plants.

    Science.gov (United States)

    Kim, Tae-Su; Han, Ji-Hye; Joung, Yochan; Kim, Seung Bum

    2015-08-01

    Two Gram-staining-positive, aerobic, endospore-forming, motile bacteria, strains DT7-4T and DLE-12T, were isolated from roots of evening primrose (Oenothera biennis) and day lily (Hemerocallis fulva), respectively, and subjected to taxonomic characterization. Analysis of 16S rRNA gene sequences indicated that the two strains fell into two distinct phylogenetic clusters belonging to the genus Paenibacillus. Strain DT7-4T was most closely related to Paenibacillus phyllosphaerae PALXIL04T and Paenibacillus taihuensis THMBG22T, with 96.3% 16S rRNA gene sequence similarity to each, and strain DLE-12T was most closely related to Paenibacillus ginsengarvi Gsoil 139T and Paenibacillus hodogayensis SGT, with 96.6 and 93.3% sequence similarity, respectively. Both isolates contained anteiso-C15 : 0 as the dominant fatty acid, meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan and MK-7 as the respiratory menaquinone. The cellular polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unidentified polar lipids. The DNA G+C contents of strains DT7-4T and DLE-12T were 50.1 ± 0.7 and 55.2 ± 0.5 mol%, respectively. The chemotaxonomic properties of both isolates were typical of members of the genus Paenibacillus. However, our biochemical and phylogenetic analyses distinguished each isolate from related species. Based on our polyphasic taxonomic analysis, strains DT7-4T and DLE-12T should be recognized as representatives of novel species of Paenibacillus, for which the names Paenibacillus oenotherae sp. nov. (type strain DT7-4T = KCTC 33186T = JCM 19573T) and Paenibacillus hemerocallicola sp. nov. (type strain DLE-12T = KCTC 33185T = JCM 19572T) are proposed.

  7. Structural Analysis of Planar sp3 and sp2 Films: Diamond-Like Carbon and Graphene Overlayers

    KAUST Repository

    Mansour, Ahmed

    2011-01-01

    pure sp2 as in graphene, carbon nanotubes and fullerenes, to pure sp3 as in diamond. Amorphous and diamond-like carbon consists of a mixture of both hybridizations. This variation in hybridization in carbon materials enables a wide spectrum

  8. Dominancy of Trichodesmium sp. in the Biawak Island

    Science.gov (United States)

    Prihadi, D. J.

    2018-03-01

    The Biawak Island is one of the small islands in West Java Province with an abundance of marine biological resources. This research was conducted to collect the primary producer zooplankton and water quality parameters. Direct observation is done by field surveys and measurement in situ for plankton and environmental parameters such as temperature, water transparency, water current, salinity, dissolved oxygen, and pH. Trichodesmium sp. was found dominance in where some other types of zooplankton were found in the area, like Scenedesmus sp., Sagitta sp., Acartia sp. also occurred. Further, the most abundance of Trichodesmium sp. was found in southern of Biawak Island where mangroves, coral and seagrass ecosystem provide nutrients which indirectly support the abundance of planktons. Trichodesmium sp. is plankton that can survive in water with minimum nutrient.

  9. Characterization and Optimization of Bioflocculant Exopolysaccharide Production by Cyanobacteria Nostoc sp. BTA97 and Anabaena sp. BTA990 in Culture Conditions.

    Science.gov (United States)

    Tiwari, Onkar Nath; Khangembam, Romi; Shamjetshabam, Minerva; Sharma, Aribam Subhalaxmi; Oinam, Gunapati; Brand, Jerry J

    2015-08-01

    Bioflocculant exopolysaccharide (EPS) production by 40 cyanobacterial strains during their photoautotrophic growth was investigated. Highest levels of EPS were produced by Nostoc sp. BTA97 and Anabaena sp. BTA990. EPS production was maximum during stationary growth phase, when nitrogenase activity was very low. Maximum EPS production occurred at pH 8.0 in the absence of any combined nitrogen source. The cyanobacterial EPS consisted of soluble protein and polysaccharide that included substantial amounts of neutral sugars and uronic acid. The EPS isolated from Anabaena sp. BTA990 and Nostoc sp. BTA97 demonstrated high flocculation capacity. There was a positive correlation between uronic acid content and flocculation activity. The flocculant bound a cationic dye, Alcian Blue, indicating it to be polyanionic. The 16S rRNA gene sequences for Nostoc sp. BTA97 and Anabaena sp. BTA990 were deposited at NCBI GenBank, and accession numbers were obtained as KJ830951 and KJ830948, respectively. The results of these experiments indicate that strains Anabaena sp. BTA990 and Nostoc sp. BTA97 are good candidates for the commercial production of EPS and might be utilized in industrial applications as an alternative to synthetic and abiotic flocculants.

  10. Atividade in vitro do extrato de própolis contra agentes bacterianos da mastite bovina In vitro activity of propolis extract against bovine mastitis bacterial agents

    Directory of Open Access Journals (Sweden)

    Andrea Pinto Loguercio

    2006-02-01

    Full Text Available Este trabalho foi desenvolvido com o objetivo de avaliar a atividade in vitro do extrato alcoólico de própolis, contra agentes da mastite bovina, comparando-o aos principais antimicrobianos utilizados no tratamento convencional. Foram utilizados 36 isolados coagulase-positivos de Staphylococcus sp. e 27 isolados de Streptococcus sp.; 94,4% dos Staphylococcus sp. e 85,2% dos Streptococcus sp. foram susceptíveis ao extrato de própolis.The present study aimed to determine the in vitro activity of propolis extract, comparing it to the most common antibacterial drugs against bovine mastitis bacterial agents. Thirty-six isolates of coagulase-positive Staphylococcus sp. and twenty-seven of Streptococcus sp. were analyzed. Coagulase-positive Staphylococcus (94.4% and Streptococcus sp. (85.2% showed susceptibility to propolis extract.

  11. Antifungal Activity of (+-2,2’-Epicytoskyrin A and Its Membrane-Disruptive Action

    Directory of Open Access Journals (Sweden)

    Dewi Wulansari

    2016-12-01

    Full Text Available (+-2,2’-Epicytoskyrin A, a bis-anthraquinone isolated from fungal endophyte Diaporthe sp. GNBP-10 associated with Uncaria gambir Roxb., was investigated for its antifungal activity. The broth microdilution method was used to determine the minimum inhibitory concentration (MIC against 22 yeast strains and three filamentous fungi. The MICs of (+-2,2’-epicytoskyrin A ranged from 16 to 128 µg/mL, which exhibited lower activity than the antifungal nystatin. A study of the mechanism of action revealed similar effects of (+-2,2’-epicytoskyrin A and nystatin on Candida tropicalis at their MICs (16 and 8 µg/mL, respectively and 2 times of the MIC. Both compounds caused cytoplasmic material and ion leakages on fungal cell, which were characterized by an increase in absorbance at 260 nm and 280 nm as well as Ca2+ and K+ ion concentrations. The morphology of the fungal cells after (+-2,2’-epicytoskyrin A treatment was observed under a scanning electron microscope. The control cells, which were not treated with either (+-2,2’-epicytoskyrin A or nystatin, showed a smooth surface, while the cells treated with either (+-2,2’-epicytoskyrin A or nystatin shrank and displayed a donut-like shape. More shrinkage was observed in the 2 times MIC concentration and even more in the cells exposed to nystatin. The action of (+-2,2’-epicytoskyrin A was proposed through membrane disruption.

  12. Checklist of the birds of Aruba, Curaçao and Bonaire, South Caribbean

    NARCIS (Netherlands)

    Prins, T.G.; Reuter, J.H.; Debrot, A.O.; Wattel, J.; Nijman, V.

    2009-01-01

    We present an updated checklist of the birds of the islands of Aruba, Curaçao and Bonaire, and the islets of Klein Curaçao and Klein Bonaire, southern Caribbean, and compare this with earlier checklists (K.H. Voous, Stud. Fauna Curaçao Carib. Isl. 7: 1-260, 1957; Ardea 53: 205-234, 1965; Birds of

  13. A Arqueologia do cuidado: da prática ao discurso

    Directory of Open Access Journals (Sweden)

    Sidi Askofare

    2006-06-01

    Full Text Available A noção de cuidado, assimilada há muito tempo ao tratamento e, então, ao conceito de terapêutica, retorna, logo depois, nos estudos, pesquisas e reflexões nas ciências humanas. Mas, daí em diante, está emancipado de suas aderências e conotações médicas por serem demasiadamente redutoras. O presente artigo propõe uma arqueologia (no sentido foucaultiano do cuidado &– de suas origens animais às suas formas culturalmente e historicamente definidas &– e põe em evidência a subversão que produz no campo do cuidado &– das práticas aos discursos &– a emergência e a dominação do discurso da ciência.

  14. Adição de probiótico ao leite integral ou sucedâneo e desempenho de bezerros da raça holandesa Addition of probiotic to whole milk or milk replacer and holstein calves performance

    Directory of Open Access Journals (Sweden)

    Paula Marques Meyer

    2001-06-01

    Full Text Available A possibilidade de proibição do uso de antibióticos como promotores de crescimento para animais de produção tem feito com que consumidores e produtores procurem por alternativas. Os probióticos têm se mostrado promissores em cumprir este papel e por esta razão, setenta e nove bezerros da raça Holandesa foram utilizados para avaliar a adição de probiótico constituído por Lactobacillus acidophilus, Enterococcus faecium e Saccharomyces cerevisiae ao aleitamento. Os animais foram distribuídos em um delineamento inteiramente casualizado, com arranjo fatorial de tratamentos 3x2, correspondendo ao tipo de dieta líquida (leite integral, sucedâneo ao 3° dia ou sucedâneo ao 15° dia de idade, com adição ou não de probiótico. O período experimental foi do nascimento até 15 dias após a desmama. O fornecimento de probiótico a bezerros(as aleitados(as com sucedâneo a partir dos 3 dias de idade melhorou o ganho de peso (com probiótico=0,22 vs. sem probiótico=0,16 kg dia-1 e conversão alimentar (2,62 vs. 3,85 até a desmama, e conversão alimentar (1,66 vs. 2,03 pós-desmama. Os bezerros aleitados com sucedâneo consumiram mais concentrado em relação aos que receberam leite integral (sucedâneo=0,22 vs. leite=0,19 kg dia-1, mas consumiram menos matéria seca total (0,61 vs. 0,67 kg dia-1 e apresentaram menor peso à desmama (49 vs. 59 kg. Quando o uso de sucedâneo foi iniciado aos 3 dias, os bezerros apresentaram maior consumo de concentrado (sucedâneo aos 3 dias=0,25 vs. sucedâneo aos 15 dias=0,20 kg dia-1 e menor peso à desmama (47,3 vs. 51 kg em relação aos bezerros aleitados com sucedâneo aos 15 dias.The possibility of limiting antibiotics as growth stimulants for farm animals has produced a climate in which both consumers and manufacturers are looking for alternatives. Probiotics are being considered to exercise this role, and for this reason, 79 newborn Holstein calves were used to evaluate the effects of

  15. Avaliação da continuidade espacial de características dendrométricas em diferentes idades de povoamentos clonais de Eucalyptus sp. Evaluation of spatial continuity of dendrometric characteristics of clonal stands of Eucalyptus sp. at different ages

    Directory of Open Access Journals (Sweden)

    Honório Kanegae Junior

    2007-10-01

    Full Text Available O conhecimento da estrutura de continuidade espacial das características dendrométricas pode propiciar um eficiente controle de variação sobre as variáveis que se deseja estimar nos inventários sucessivos. Esse controle pode ser efetivado pelo uso de estratificadores baseados em mapas resultantes da krigagem estatística. Entretanto, o uso da krigagem carece de informações sobre a questão da continuidade espacial das variáveis descritoras dos povoamentos de Eucalyptus sp., assim como o comportamento dessas características no tempo. Este estudo teve como objetivo avaliar a estrutura de continuidade espacial de três características dendrométricas de 23 povoamentos clonais de Eucalyptus sp. em três medições sucessivas, localizados em regiões distintas do Estado de São Paulo. Para cada povoamento, variável e medição, ajustaram-se semivariogramas experimentais pelo método dos Quadrados Mínimos Ponderados. Através do grau de dependência espacial obtido pelos semivariogramas experimentais, avaliou-se o comportamento da continuidade espacial das variáveis. Mais de 70% dos povoamentoss analisados apresentaram de média a forte dependência espacial para volume e 80% para as variáveis área basal e altura dominante. O alcance das variáveis apresentou variações de 300 a 3.000 m, dependendo do povoamento e da idade de medição. O porcentual de povoamentos com forte dependência espacial decresce com a idade, tendendo ao patamar de média dependência espacial, em todas as variáveis analisadas. O grau de dependência espacial ao longo de sucessivas medições variou de povoamento para povoamento e de medições para medições, podendo diminuir ou aumentar com o tempo, indicando que em cada povoamento e medição seja feita uma análise específica da continuidade espacial da variável de interesse.The knowledge on the structure of spatial continuity of dendrometric characteristics can provide an efficient control of

  16. Peso ao nascer e mortalidade hospitalar entre nascidos vivos, 1975-1996 Birth weight and hospital mortality among the newborns in maternity, 1975-1996

    Directory of Open Access Journals (Sweden)

    Gladys GB Mariotoni

    2000-02-01

    Full Text Available OBJETIVO: Conhecer a evolução do peso ao nascer e a mortalidade hospitalar de nascidos vivos em maternidade de Campinas, SP. MÉTODOS: Foram estudados 215.435 nascimentos ocorridos em maternidade do Município de Campinas, SP, de 1975 a 1996. As informações foram coletadas de livros de registro e relatórios de assistência médica. Foram analisados os óbitos ocorridos antes da alta hospitalar, independentemente da idade. Para a análise dos dados, utilizou-se o programa Epi Info 6.01. RESULTADOS: O baixo peso se manteve próximo a 9% desde os anos 70, concentrando82% dos óbitos hospitalares em 1996. O peso insuficiente foi observado em 24% dos nascidos vivos. Em 1975, 13,3% dos nascidos com peso entre 1.000 g e 1.499 g sobreviveram e 73,8% dos nascidos com peso entre 1.500 g e 1.999 g. Em 1996, a sobrevida foi 69,1% e 87,7%, respectivamente. Houve grande diminuição do número de óbitos especialmente na faixa de 1.000 g a 1.499 g (de 867 para 309 por mil nascidos vivos, redução de 64,5% e de 1.500 g a 1.999 g (de 264 para 123 por mil nascidos vivos, redução de 53,4%. A mortalidade hospitalar entre aqueles de peso abaixo de 1.000 g permaneceu entre 78,9% e 100,0%, mesmo nos anos 90. Observou-se queda da mortalidade hospitalar de 17 para 11 por mil nascidos vivos. CONCLUSÕES: Não houve modificação na distribuição do peso ao nascer, a despeito deavanços apresentados por Campinas. Contudo, observou-se queda da mortalidade hospitalar. Outros estudos são necessários para compreender esse perfil.OBJECTIVE: To assess the trend of birth weight and hospital mortality rate of newborns, in a maternity hospital of Campinas, SP. METHODS: A total of 215,435 births of a maternity hospital in Campinas, S. Paulo State, Brazil, was studied between 1976 and 1996. Information was collected from registration files and annual reports of medical care. All the newborns from that period were included. The causes of death that occurred before the

  17. Os solos como indicadores das relações entre sedimentos continentais e marinhos na planície costeira: Rio Guaratuba (SP

    Directory of Open Access Journals (Sweden)

    M. Rossi

    2001-03-01

    Full Text Available Foram estudadas duas toposseqüências na planície litorânea no rio Guaratuba (SP, definindo seqüência de evolução pedológica e de deposição/acumulação, utilizando a análise macromorfológica de solos por meio de tradagens. A primeira seqüência ilustra a transformação de Espodossolo Ferrocárbico (Podzol sobre sedimentos arenosos marinhos para Organossolo (solo orgânico, enquanto a outra mostra a relação entre Gleissolo Háplico (solo glei pouco húmico sobre sedimentos continentais e Espodossolo Ferrocárbico (Podzol em sedimentos areno-quartzosos marinhos. A sedimentação marinha é representada por feixes de restinga arenosos, com zonas embaciadas, propiciando o desenvolvimento de Espodossolos nas partes altas e Organossolos nas partes baixas. Morros isolados inferiores à planície funcionaram como antigas zonas de balizamento desses feixes, que limitaram o sistema deposicional continental, definindo o material marinho como anterior ao continental, onde ocorreu um encaixamento da drenagem próximo ao contato.

  18. Statistical evaluation of nutritional components impacting phycocyanin production in Synechocystis SP

    Directory of Open Access Journals (Sweden)

    Devendra V. Deshmukh

    2012-03-01

    Full Text Available Alkaliphilic cyanobacterial cultures were isolated from Lonar lake (MS, India. Among the set of cultures, Synechocystis sp, was studied for phycocyanin production. A maximum yield was obtained in BG-11 medium at optimized conditions (pH 10 and 16 h light. In order to increase the phycocyanin yield media optimization based on the eight media components a Plackett-Burman design of the 12 experimental trials was used. As per the analysis CaCl2.2H2O and Na2CO3 have been found to be the most influencing media components at 95% significance. Further the optimum concentrations of these components were estimated following a Box Wilson Central Composite Design (CCD with four star points and five replicates at the center points for each of two factors was adopted for optimization of these two media components. The results indicated that there was an interlinked influence of CaCl2.2H2O and Na2CO3 on 98% significance. The maximum yield of phycocyanin (12% of dry wt could be obtained at 0.058 g/l and 0.115 g/l of CaCl2.2H2O and Na2CO3, respectively.

  19. Antibodies against spotted fever group Rickettsia sp., in horses of the colombian Orinoquia

    Directory of Open Access Journals (Sweden)

    Diego A. Riveros-Pinilla

    2015-11-01

    Full Text Available Objective. It was determined the presence of antibodies against Rickettsia sp. of the spotted fever group, in horses of 8 municipalities of the Colombian Orinoquia. Matherials and methods. A cross-sectional study was conducted on 246 sera from apparently healthy horses and processed by the indirect immunofluorescence test (IFI. Results. General seropositivity was (2.85%; 7/246, while by municipalities the results were, Arauca (9.1%; 2/22, Saravena (5.6%; 1/18, San José del Guaviare (4.9%; 2/41, San Martín (3.8%; 1/26, Yopal (1.9%; 1/52. It was not identified the presence of antibodies in Puerto López (0/52, Puerto Gaitán (0/15 and Villavicencio (0/20. Four of the positive samples presented titles of 1:64, while the remaining 3 1:128. Conclusions. It shows the circulation of Rickettsia sp. of the Spotted Fever Group in horses in the region of the Colombian Orinoquia, suggesting the need for further studies to understand the ecoepidemiology of municipalities with presence of seropositive.

  20. Desempenho de vacas Charolês e Nelore desterneiradas aos três ou sete meses

    Directory of Open Access Journals (Sweden)

    Restle João

    2001-01-01

    Full Text Available Foi avaliado o desempenho de vacas Charolês (C e Nelore (N, agrupadas em três classes de idade, jovens (3 e 4 anos, adultas (5 a 7 anos e velhas (8 ou mais anos, desmamadas aos três (precoce ou sete meses no outono (tradicional. O peso no outono das vacas desterneiradas aos três meses (T3 foi 45 kg superior ao das vacas com remoção do bezerro aos sete meses (T7. O estado corporal aos sete meses também foi melhor nas vacas do T3 (3,3 contra 2,1 pontos. Vacas do T3 apresentaram maior ganho de peso do parto ao final do período reprodutivo e apresentaram maiores porcentagem de cio (81 contra 51% e prenhez (67,2 contra 37,3% e menor intervalo do parto ao primeiro cio pós-parto (102 contra 114 dias que vacas do T7. Vacas adultas apresentaram melhor estado corporal aos sete meses e tiveram melhor desempenho reprodutivo do que vacas velhas e jovens. A diferença na porcentagem de prenhez entre o T3 e T7 foi mais evidente nas vacas jovens (42,11 contra 12,5% e velhas (51,72 contra 35,71% que nas adultas (62,50 contra 53,33%. Vacas C foram mais pesadas que as N, ao parto, aos três e sete meses pós-parto e apresentaram melhor estado corporal aos sete meses. O efeito do desmame precoce no desempenho reprodutivo foi mais evidente nas vacas C. A porcentagem de fêmeas prenhes nas C foi de 80,60% para o T3 e 41,90% para o T7, já nas N as porcentagens foram de 45,50 e 30,00%, respectivamente, para o T3 e T7. Nas vacas C, a produção de leite e a amamentação apresentaram efeito inibidor, sobre a reprodução, mais marcante que nas vacas N.

  1. Fracture morphology of AO/OTA 31-A trochanteric fractures: A 3D CT study with an emphasis on coronal fragments.

    Science.gov (United States)

    Cho, Jae-Woo; Kent, William T; Yoon, Yong-Cheol; Kim, Youngwoo; Kim, Hyungon; Jha, Ashutosh; Durai, Senthil Kumar; Oh, Jong-Keon

    2017-02-01

    This study was designed to assess the incidence and morphology of coronal plane fragments in AO/OTA 31-A trochanteric fractures. 156 cases of AO/OTA 31-A trochanteric fractures were retrospectively evaluated. Lateral radiographs were analyzed for the presence of coronal plane fragments followed by analysis of 3D CT reconstructions in these fractures. The incidence of coronal fragments identified on the lateral radiograph and 3D CT reconstructions were both calculated. Coronal fragment morphology was described based upon the origin and exit points of fracture lines and the number of fragments. On plain radiographs, a coronal plane fracture was identified in 59 cases, an incidence of 37.8% (59/156). In comparison, 3D CT reconstructions identified coronal plane fractures in 138 cases for an incidence of 88.4% (138/156). 3D CT reconstructions identified coronal fracture fragments in 81.9% (50/61) of AO/OTA 31-A1 cases, 94.5% (69/73) of 31-A2 cases, and 86.3% (19/22) of 31-A3 cases. Incidence of coronal fractures identified on plain radiographs of 3 AO/OTA 31-A1,A2,A3 groups was lower when compared to the incidence of coronal fractures identified on 3D CT. Of the 138 cases that had coronal plane fracture, 82 cases (59.4%) had a single coronal fragment (GT fragment 35 cases, GLT fragment 19 cases, GLPC fragment 28 cases). The remaining 56 cases (40.5%) had two coronal fragments. There is a high incidence of coronal fragments in intertrochanteric femur fractures when analyzed with 3D CT reconstructions. Our study suggests that these coronal fragments are difficult to identify on plain radiographs. Knowledge of the incidence and morphology of coronal fragments helps to avoid potential intraoperative pitfalls. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Subsídios para implantação do processo de compostagem em município de pequeno porte : estudo de caso em Corumbataí-SP

    OpenAIRE

    Lucilene de Aquino

    2012-01-01

    A compostagem é um dos mais antigos processos de reciclagem de resíduos orgânicos. Apesar de ser um tratamento ambientalmente adequado, sua utilização ainda é pouco difundida no que se refere à gestão municipal dos resíduos sólidos urbanos (RSU). O presente trabalho de pesquisa teve como objetivo investigar a gestão dos RSU no município de Corumbataí-SP. Para isso, foi realizada caracterização gravimétrica dos RSU e foram analisadas alternativas de compostagem aplicáveis ao município. O estud...

  3. Como responder ao momento presente?

    Directory of Open Access Journals (Sweden)

    Maria Filomena Molder

    2013-12-01

    Full Text Available http://dx.doi.org/10.5007/1984-784X.2013v13n19p13 Foi com esta pergunta — já um efeito de um primeiro encontro entre Irene Pimentel e eu própria — que decidimos desafiar colegas, estudantes e funci­onários da nossa Faculdade, FCSH (Faculdade de Ciências Sociais e Huma­nas, de outras Faculdades da Universidade Nova de Lisboa, de outras Uni­versidades e todos os interessados em con­siderar e discutir em comum aquilo que se passava em Portugal e que no anúncio da Jornada de 6 de De­zembro de 2012 se descrevia como um “processo de desmantela­mento social, económico e cultural sem precedentes — pese embora tantas compara­ções, baseadas na premissa da ‘eterna repetição’ — e cujas consequências não param de exceder as previsões dos responsáveis por esse desmantelamento”. Acedendo com todo o empenho e gratidão ao convite que me foi dirigido por Humberto Brito para fazer uma resenha da Jornada a publicar no primeiro número de Forma de Vida (saúdo a revista e o título, decidi-me, no entanto, a pôr de lado a resenha, que sob a forma de “Editorial” será em breve publi­cada no blogue Responder ao Momento Presente, entre­tanto criado, conjuntamente com os textos escritos pelos nossos convidados, com as parti­cipações de pessoas que corresponderam ao nosso apelo e ainda com contri­bui­ções que se alargaram para lá da Jornada; a que se juntará uma gravação em video, também disponível no Youtube.   Texto publicado originalmente em Forma de Vida, Lisboa, n.1, fev. 2013. Agrade­cemos à autora por permitir a republicação neste número do Boletim. [N.E.

  4. Resistance to stem canker, frogeye leaf spot and powdery mildew of soybean lines lacking lipoxigenases in the seeds Resistência ao cancro-da-haste, à cercosporiose e ao oídio de linhagens de soja sem lipoxigenases nas sementes

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Osório Martins

    2002-12-01

    ãos. A presença de gosto de feijão cru tem sido limitante para o consumo de derivados de soja pelos povos ocidentais. Esse sabor característico é proporcionado pelas enzimas lipoxigenases (Lox1, Lox2 e Lox3. A eliminação dessas enzimas, pela manipulação genética dos alelos que as codificam, é a maneira mais adequada de contornar os problemas associados ao sabor desagradável. Visando elucidar a participação das lipoxigenases, no processo de resistência da soja a patógenos, variedades normais de soja (FT-Cristalina RCH, Doko RC e IAC-12 e suas respectivas linhagens obtidas por retrocruzamentos, sem as três lipoxigenases nas sementes (triplo-nulas - TN e com as três lipoxigenases (triplo-positivas - TP, foram testadas quanto às suas resistências ao cancro-da-haste (Diaporthe phaseolorum f.sp. meridionalis, à cercosporiose (Cercospora sojina Hara e ao oídio (Microsphaera diffusa Cke. & Pk.. Todos os materiais genéticos foram resistentes ao cancro-da-haste. Com relação à cercosporiose, FT-Cristalina RCH e Doko-RC e suas respectivas linhagens com ou sem lipoxigenases mostraram-se resistentes, enquanto IAC-12 e suas linhagens derivadas mostraram índices de doença mais elevados, sendo que as linhagens IAC-12 TP e TN foram mais suscetíveis, indicando perda de gene de resistência nos retrocruzamentos. Não houve relação entre retirada dos genes que codificam lipoxigenases nas sementes com a resistência à cercosporiose. No caso do oídio, as linhagens TP ou TN apresentaram-se similares ou pouco mais resistentes que seus respectivos progenitores recorrentes, os quais se mostraram suscetíveis na seguinte ordem: IAC-12, menos suscetível, Doko-RC, intermediário e FT-Cristalina RCH, mais suscetível.

  5. Performance of a MEMS-based AO-OCT system using Fourier Reconstruction

    Energy Technology Data Exchange (ETDEWEB)

    Evans, J; Zawadzki, R; Jones, S; Olivier, S; Werner, J S

    2009-01-21

    Adaptive optics (AO) and optical coherence tomography (OCT) are powerful imaging modalities that, when combined, can provide high-resolution (3.5 {micro}m isotropic), 3-D images of the retina. The AO-OCT system at UC Davis has demonstrated the utility of this technology for microscopic, volumetric, in vivo retinal imaging. The current system uses an AOptix bimorph deformable mirror (DM) for low-order, high-stroke correction and a 140-actuator Boston Micromachines DM for high-order correction. Developments to improve performance or functionality of the instrument are on-going. Based on previous work in system characterization we have focused on improved AO control. We present preliminary results and remaining challenges for a newly implemented Fourier transform reconstructor (FTR). The previously reported error budget analysis is also reviewed and updated, with consideration of how to improve both the amount of residual error and the robustness of the system. Careful characterization of the AO system will lead to improved performance and inform the design of future systems.

  6. Biotransformation of the high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by Sphingobium sp. strain KK22 and identification of new products of non-alternant PAH biodegradation by liquid chromatography electrospray ionization tandem mass spectrometry.

    Science.gov (United States)

    Maeda, Allyn H; Nishi, Shinro; Hatada, Yuji; Ozeki, Yasuhiro; Kanaly, Robert A

    2014-03-01

    A pathway for the biotransformation of the environmental pollutant and high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by a soil bacterium was constructed through analyses of results from liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(-)-MS/MS). Exposure of Sphingobium sp. strain KK22 to benzo[k]fluoranthene resulted in transformation to four-, three- and two-aromatic ring products. The structurally similar four- and three-ring non-alternant PAHs fluoranthene and acenaphthylene were also biotransformed by strain KK22, and LC/ESI(-)-MS/MS analyses of these products confirmed the lower biotransformation pathway proposed for benzo[k]fluoranthene. In all, seven products from benzo[k]fluoranthene and seven products from fluoranthene were revealed and included previously unreported products from both PAHs. Benzo[k]fluoranthene biotransformation proceeded through ortho-cleavage of 8,9-dihydroxy-benzo[k]fluoranthene to 8-carboxyfluoranthenyl-9-propenic acid and 9-hydroxy-fluoranthene-8-carboxylic acid, and was followed by meta-cleavage to produce 3-(2-formylacenaphthylen-1-yl)-2-hydroxy-prop-2-enoic acid. The fluoranthene pathway converged with the benzo[k]fluoranthene pathway through detection of the three-ring product, 2-formylacenaphthylene-1-carboxylic acid. Production of key downstream metabolites, 1,8-naphthalic anhydride and 1-naphthoic acid from benzo[k]fluoranthene, fluoranthene and acenaphthylene biotransformations provided evidence for a common pathway by strain KK22 for all three PAHs through acenaphthoquinone. Quantitative analysis of benzo[k]fluoranthene biotransformation by strain KK22 confirmed biodegradation. This is the first pathway proposed for the biotransformation of benzo[k]fluoranthene by a bacterium. © 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  7. Biotransformation of the high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by Sphingobium sp. strain KK22 and identification of new products of non-alternant PAH biodegradation by liquid chromatography electrospray ionization tandem mass spectrometry

    Science.gov (United States)

    Maeda, Allyn H; Nishi, Shinro; Hatada, Yuji; Ozeki, Yasuhiro; Kanaly, Robert A

    2014-01-01

    A pathway for the biotransformation of the environmental pollutant and high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by a soil bacterium was constructed through analyses of results from liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(–)-MS/MS). Exposure of Sphingobium sp. strain KK22 to benzo[k]fluoranthene resulted in transformation to four-, three-and two-aromatic ring products. The structurally similar four-and three-ring non-alternant PAHs fluoranthene and acenaphthylene were also biotransformed by strain KK22, and LC/ESI(–)-MS/MS analyses of these products confirmed the lower biotransformation pathway proposed for benzo[k]fluoranthene. In all, seven products from benzo[k]fluoranthene and seven products from fluoranthene were revealed and included previously unreported products from both PAHs. Benzo[k]fluoranthene biotransformation proceeded through ortho-cleavage of 8,9-dihydroxy-benzo[k]fluoranthene to 8-carboxyfluoranthenyl-9-propenic acid and 9-hydroxy-fluoranthene-8-carboxylic acid, and was followed by meta-cleavage to produce 3-(2-formylacenaphthylen-1-yl)-2-hydroxy-prop-2-enoic acid. The fluoranthene pathway converged with the benzo[k]fluoranthene pathway through detection of the three-ring product, 2-formylacenaphthylene-1-carboxylic acid. Production of key downstream metabolites, 1,8-naphthalic anhydride and 1-naphthoic acid from benzo[k]fluoranthene, fluoranthene and acenaphthylene biotransformations provided evidence for a common pathway by strain KK22 for all three PAHs through acenaphthoquinone. Quantitative analysis of benzo[k]fluoranthene biotransformation by strain KK22 confirmed biodegradation. This is the first pathway proposed for the biotransformation of benzo[k]fluoranthene by a bacterium. PMID:24325265

  8. Estados Unidos: do Iraque ao Brasil

    Directory of Open Access Journals (Sweden)

    Virgílio Caixeta Arraes

    2010-12-01

    Full Text Available O presente artigo busca analisar os recentes temas recorrentes na política externa norte-americana, caracterizada pela “guerra contra o terror”, e seus impactos para a agenda a ser discutida na vindoura visita do presidente dos Estados Unidos, Geoge W. Bush, ao Brasil.

  9. SIG aplicado ao Ensino de Geografia

    OpenAIRE

    Dornelles, Liane Maria Azevedo; Universidade do Estado do Rio de Janeiro - UERJ

    2009-01-01

    Este trabalho descreve as atividades desenvolvidas junto à disciplina eletiva SIG aplicado ao ensino de Geografia. Foram elaboradas aplicações ambientais para os Ensinos Fundamental e Médio, com auxílio dos programas VistaSAGA/UFRJ, SISPLAMTE 5as com GIS, SPRING/INPE e MapServer.

  10. Detection of hemoplasma and Bartonella species and co-infection with retroviruses in cats subjected to a spaying/neutering program in Jaboticabal, SP, Brazil Detecção de hemoplasmas e Bartonella sp. e co-infecção com retrovírus em gatos submetidos a um programa de castração/esterilização em Jaboticabal, SP, Brasil

    Directory of Open Access Journals (Sweden)

    Caroline Plácidi de Bortoli

    2012-09-01

    Full Text Available Hemotrophic mycoplasmas and Bartonella species are important pathogens that circulate between cats and invertebrate hosts, occasionally causing diseases in humans. Nevertheless, there are few reports on occurrences of these agents in cats in Brazil. The present study aimed to detect the presence of hemoplasma and Bartonella DNA by means of PCR and sequencing. FIV antigens and anti-FeLV antibodies, were studied by using a commercial kit on blood and serum samples, respectively, among 46 cats that were sampled during a spaying/neutering campaign conducted in Jaboticabal, SP. Three (6.5% cats were positive for hemoplasmas: two (4.3% for 'Candidatus M. haemominutum' and one (2.2% for both M. haemofelis and 'Candidatus M. turicensis'. One of the two 'Candidatus M. haemominutum'-infected cats was also positive for FeLV antigens and showed antibodies for FIV. Two cats (4.3% were positive for B. henselae. One of them was also positive for FeLV antigens. Eight cats (17.4% were positive for FeLV, and just one (2.2% showed anti-FIV antibodies. Bartonella species and hemoplasmas associated with infection due to retroviruses can circulate among apparently healthy cats.Micoplasmas hemotróficos e espécies de Bartonella são importantes patógenos que circulam entre gatos e hospedeiros invertebrados, causando ocasionalmente doenças no homem. Apesar disto, poucos são os estudos acerca da ocorrência destes agentes entre gatos no Brasil. O presente estudo objetivou detectar o DNA de hemoplasmas e Bartonella sp. pela PCR e sequenciamento. Antígeno de FIV e anticorpos anti-FeLV foram estudados utilizando um "kit" comercial, em amostras de sangue e soro, respectivamente, de 46 gatos amostrados em uma campanha de castração em Jaboticabal, SP. Três gatos (6,5% foram positivos para hemoplasmas: dois (4,3% para 'Candidatus M. haemominutum' e um (2,2% para M. haemofelis and 'Candidatus M. turicensis'. Um dos gatos positivos para 'Candidatus M. haemominutum

  11. Doença do corpúsculo de inclusão e espondilite por Salmonella sp. em uma Boa constrictor constrictor

    Directory of Open Access Journals (Sweden)

    Isabela Hardt

    Full Text Available RESUMO: Doença do corpúsculo de inclusão (IBD é uma enfermidade caracterizada por corpúsculos intracitoplasmáticos em diversos tecidos, principalmente no sistema nervoso central, responsável pelos principais sinais clínicos atribuídos à doença que acomete Boas e Phytons de cativeiro; essa enfermidade tem sido uma preocupação mundial devido à alta morbidade e mortalidade. O diagnóstico é feito pela visualização dos corpúsculos causados por um Arenavírus modificado. Salmonella sp. pertence à microflora de animais de sangue frio e quente, e é um patógeno oportunista que pode causar quadros gastrointestinais ou septicêmicos. Em répteis a Salmonella sp. é a bactéria com maior frequência de citações em espondilites e osteomielites. Relata-se um caso de uma jiboia (Boa constrictor constrictor que apresentava restrição de movimento e múltiplos granulomas dorsais nas vértebras; à radiografia evidenciaram-se regiões fraturadas. Após meses de tratamentos sem melhora clínica e o aparecimento de novas lesões o animal ficou prostrado, anoréxico, caquético e desenvolveu opistótono; optou-se pela eutanásia. À necropsia verificaram-se, nas vértebras, múltiplos focos dorsais com aumento de volume que variava de 1,7cm à 3,8cm. Ao corte as vértebras eram deformadas e exibiam conteúdo caseoso focal próximo ao canal medular, este foi coletado para microbiologia onde se identificou Salmonella sp. À microscopia as vértebras tinham um infiltrado inflamatório multifocal moderado de macrófagos e heterofilos. Algumas áreas possuíam grande quantidade de granulomas com calcificação central e inúmeras células gigantes; outros mostravam áreas de osteomalácia e fibrose. Em raros focos havia fratura do corpo vertebral e compressão da medula espinhal com leve infiltrado inflamatório invadindo o canal medular. No pulmão, principalmente no epitélio brônquico, por vezes até dentro de linfócitos do tecido linfoide

  12. Detection of Entamoeba sp. and Helmith Eggs From Water Sources in Urban Slum Area in Bandung Municipality

    Directory of Open Access Journals (Sweden)

    Sri Yusnita Irda Sari

    2017-09-01

    Full Text Available Diarrhea is a waterborne disease due to consumption of contaminated food/water. People in urban slum area have highest risk to get diarrhea because of poor hygiene and sanitation as well as limited access to uncontaminated water. This study aimed to identify conta­mination in watersources by Entamoeba Sp and helmint eggs (Anchylostoma duodenale, Ascaris lumbricoides, Trichuris trichuria in one of urban slum area in Bandung municipality. Samples were taken from 123 watersources (74 tap water, 21 borehole, 22 dugwell and 6 spring water which was randomly selected in 10 RW along the Cikapundung river basin in Tamansari subdistrict during period of July-September 2015. Water samples were examined by PCR to detect Entamoeba Sp and microscopic identification for helminth eggs. 90 out of 123 samples were positive for Entamoeba Sp (59 tap water, 16 dugwell, 11 borehole and 4 spring water. Helminth egg of Ascaris lumbricoides was detected from unimproved common dugwell which had very high risk of contamination. Appropriate of water treatment prior to consumption is vastly important. Physical improvement to construct improved dugwell should be done to prevent contamination from helminth eggs in watersources.

  13. NEGRITUDE E UNIVERSIDADE: EVIDENCIANDO QUESTÕES RELACIONADAS AO INGRESSO E AOS PROJETOS CURRICULARES. IOLANDA OLIVEIRA (ORGANIZADORA, 2015

    Directory of Open Access Journals (Sweden)

    Joselina da Silva

    2017-04-01

    Full Text Available A década de setenta marca um dos momentos inauguradores, no Brasil, da constituição do corpo como bandeira e afirmação identitária por parte dos movimentos sociais negros. A negritude, em performance inspirada nos movimentos brasileiros dos anos trinta, em São Paulo, tomou a rua nas diversas capitais do país. Eram os movimentos Black Soul e os blocos afros. Os primeiros, aludiam diretamente aos afro estadunidenses; os segundos, por sua vez, cunhados por uma juventude cultural afro-baiana, instituíam uma moda capilar advinda dos trançados de diferentes países do continente africano, somados aos dreadlocks jamaicanos rematados com as roupas trajadas pelos orixás do panteão yorubá, das casas de culto ancestral. Nessa ambiência é que se destaca o protagonismo da intelectual que organiza o livro Negritude e universidade.

  14. ROBO-AO M DWARF MULTIPLICITY SURVEY

    Science.gov (United States)

    Lamman, Claire; Berta-Thompson, Zachory; Baranec, Christoph; Law, Nicholas; Schonhut, Jessica

    2018-01-01

    We analyzed over 7,000 observations from Robo-AO’s field M dwarf survey taken on the 2.1m Kitt Peak telescope. Results will help determine the multiplicity fraction of M dwarfs as a function of primary mass, which is a crucial step towards understanding their evolution and formation mechanics. Through its robotic, laser-guided, and automated system, the Robo-AO instrument has yielded the largest adaptive-optics M dwarf survey to date. I developed a graphical user interface to quickly analyze this data. Initial data analysis included assessing data quality, checking the result from Robo-AO’s automatic reduction pipeline, and determining existence as well as the relative position of companions through a visual inspection. This program can be applied to other datasets and was successfully tested by re-analyzing observations from a separate Robo-AO survey. Following the preliminary results from this data analysis tool, further observations were done with the Keck II telescope by using its NIRC2 imager to follow up on ten select targets for the existence and physical association of companions. After a conservative initial cut for quality, 356 companions were found within 4” of a primary star out of 2,746 high quality Robo-AO M dwarf observations, including four triple systems. We will present a preliminary estimate for the multiplicity rate of wide M dwarf companions after accounting for observation limitations and the completeness of our search. Future research will yield insights into low-mass stellar formation and provide a database of nearby M dwarf multiples that will potentially assist ongoing and future surveys for planets around these stars, such as the NASA TESS mission.

  15. Estudo das indicações de partos cesáreas entre hospitais de atendimento ao Sistema Único de Saúde: baixo e alto riscos

    Directory of Open Access Journals (Sweden)

    Caroline Rosa Pellicciari

    2015-10-01

    Full Text Available A alta incidência de partos cesáreas  sem indicações precisas é motivo de preocupação mundial.  As cesáreas sem indicações médicas estão associadas a maiores riscos maternos e fetais. De acordo com a Organização Mundial da Saúde (OMS não há justificativa, para qualquer região do mundo possuir  taxas de partos cesáreas maiores do que 10-15%.  Este projeto investigou  os fatores indicativos para a  realização de partos cesáreas  entre hospitais da rede SUS em Sorocaba-SP: Hospital Santa Lucinda – HSL (baixo risco e Conjunto Hospitalar de Sorocaba – CHS (alto risco. Durante o período de 03/2014 a 03/2015 foi realizada análise retrospectiva de 300 prontuários das pacientes internadas nestes hospitais. Analisou-se parâmetros maternos sócio-epidemiológicos, reprodutivos, clínicos e  indicações dos partos cesáreas.  No CHS prevaleceu o tipo de parto cesárea (56,3%, enquanto no HSL a porcentagem do parto cesárea foi 32,4% (p<0,001.  Em relação ao tipo de parto anterior, o número de pacientes com cesárea anterior prevalece no HSL: 77% (n=83 em comparação com o CHS: 56,4% (n=62, (p=0,0009.   Em relação às indicações de cesárea, as mais prevalentes no HSL foram: sofrimento fetal agudo: 24% (n=36, falha na indução ao parto: 22% (n=33 e desproporção céfalo-pélvica: 18,7% (n=28. No CHS  as maiores proporções foram: sofrimento fetal agudo: 35,3% (n=53  e iteratividade: 25,3% (n=38 (p<0,0001.  A observação dos resultados demonstra a realização de partos cesáreas em porcentagens superiores àquelas preconizadas pela OMS. Sua redução poderá ser conseguida preparando-se e conscientizando-se melhor a mulher perante a futura parturição, com procedimentos precisos de indução ao parto, utilizando a aplicação de procedimentos analgésicos, e, ainda com a devida prática da extração baixa pelo fórcipe de alívio às parturientes.

  16. Two new species of suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov. epizoic on ostracods

    Digital Repository Service at National Institute of Oceanography (India)

    Santhakumari, V.

    Two, species of protozoic suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov., are described. These were found attached on the body of the marine ostracod, Cypridina dentata (Muller), collected from the shelf and slope...

  17. Down-regulation of human topoisomerase IIα expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regions

    Directory of Open Access Journals (Sweden)

    Isaacs Richard J

    2007-05-01

    Full Text Available Abstract Background Topoisomerase IIα has been shown to be down-regulated in doxorubicin-resistant cell lines. The specificity proteins Sp1 and Sp3 have been implicated in regulation of topoisomerase IIα transcription, although the mechanism by which they regulate expression is not fully understood. Sp1 has been shown to bind specifically to both proximal and distal GC elements of the human topoisomerase IIα promoter in vitro, while Sp3 binds only to the distal GC element unless additional flanking sequences are included. While Sp1 is thought to be an activator of human topoisomerase IIα, the functional significance of Sp3 binding is not known. Therefore, we sought to determine the functional relationship between Sp1 and Sp3 binding to the topoisomerase IIα promoter in vivo. We investigated endogenous levels of Sp1, Sp3 and topoisomerase IIα as well as binding of both Sp1 and Sp3 to the GC boxes of the topoisomerase IIα promoter in breast cancer cell lines in vivo after short term doxorubicin exposure. Results Functional effects of Sp1 and Sp3 were studied using transient cotransfection assays using a topoisomerase IIα promoter reporter construct. The in vivo interactions of Sp1 and Sp3 with the GC elements of the topoisomerase IIα promoter were studied in doxorubicin-treated breast cancer cell lines using chromatin immunoprecipitation assays. Relative amounts of endogenous proteins were measured using immunoblotting. In vivo DNA looping mediated by proteins bound at the GC1 and GC2 elements was studied using the chromatin conformation capture assay. Both Sp1 and Sp3 bound to the GC1 and GC2 regions. Sp1 and Sp3 were transcriptional activators and repressors respectively, with Sp3 repression being dominant over Sp1-mediated activation. The GC1 and GC2 elements are linked in vivo to form a loop, thus bringing distal regulatory elements and their cognate transcription factors into close proximity with the transcription start site

  18. Instrumento mensurador de adesao para hipertensos: contribuicao da Teoria da Resposta ao Item

    Directory of Open Access Journals (Sweden)

    Malvina Thais Pacheco Rodrigues

    2013-06-01

    Full Text Available OBJETIVO: Analisar instrumento de medição da adesão ao tratamento da hipertensão por meio da "Teoria da Resposta ao Item". MÉTODOS: Estudo analítico com 406 hipertensos com complicações associadas, atendidos na atenção básica em Fortaleza, CE, 2011, pela "Teoria da Resposta ao Item". As etapas de execução foram: teste de dimensionalidade, calibração dos itens; tratamento dos dados e construção da escala, analisadas com base no modelo de resposta gradual. Estudo da dimensionalidade do instrumento foi feito pela análise da matriz de correlação policórica e análise fatorial de informação completa. Utilizou-se o software Multilog para calibração dos itens e estimação dos escores. RESULTADOS: Os itens referentes ao tratamento medicamentoso foram os mais diretamente relacionados à adesão, enquanto aqueles referentes ao tratamento não medicamentoso precisam ser reformulados, pois possuíram menor quantidade de informação psicométrica e baixa discriminação. A independência dos itens, o reduzido número de níveis da escala e as baixas variâncias explicadas no ajuste dos modelos mostraram as principais fragilidades do instrumento analisado. A "Teoria da Resposta ao Item" mostrou-se relevante para análise, pois avaliou o respondente quanto à adesão ao tratamento da hipertensão, ao nível de dificuldade dos itens e à sua capacidade de discriminação entre indivíduos com diferentes níveis de adesão, o que gerou maior quantidade de informação. CONCLUSÕES: O instrumento analisado é limitado para medir a adesão ao tratamento da hipertensão, mediante análise pela "Teoria da Resposta ao Item", e necessita de ajustes. A adequada formulação dos itens é importante para medir precisamente o traço latente desejado.

  19. Produção de galactooligossacarídeo por Scopulariopis sp. Production of galactooligosaccharide by Scopulariopis sp.

    Directory of Open Access Journals (Sweden)

    Rosângela dos Santos

    2009-09-01

    Full Text Available Os galactooligossacarídeos (GOS são um grupo de oligossacarídeos não digeríveis (NDOs, resistentes às enzimas digestivas do intestino com efeitos similares ao da fibra. Sua ingestão aumenta seletivamente o crescimento das Bifidobacterium e dos Lactobacilus no intestino. O benéfico da ingestão de galactooligossacarídeos ocorre pelo aumento dessas populações de bifidobactérias no cólon, suprimindo a atividade de bactérias putrefativas e reduzindo a formação de produtos tóxicos por fermentação. A cepa de Scopulariopsis sp. apresentou boa produtividade de β-galactosidase quando crescida em meio de fermentação semissólida. O objetivo deste trabalho foi extrair a enzima β-galactosidase produzida por uma linhagem de Scopulariopsis sp. e avaliar o efeito da temperatura, tempo de reação, concentração de enzima e lactose para produção de GOS. Foram analisadas as temperaturas 35, 45 e 60 °C, os tempos de reação 12, 24 e 48 horas, concentrações de enzima 0,5 a 10 U.mL-1 e 10, 25 e 40% (p/v de solução de lactose (em 0,1 M de tampão acetato de sódio, pH 5.0. As condições ótimas foram de 40% (p/v, a 45 °C, 10 U.mL-1 de enzima e o melhor tempo foi de 12 horas de reação. Nessas condições, a enzima converteu 20% de lactose em oligossacarídeos (80,8 mg.mL-1 de 4'galactosyl-lactose.The galactooligosaccharides (GOS, a group of oligosaccharides, are not digerible carbohydrates (NDOs and are resistant to hydrolysis by intestine digestive enzymes with similar dietary fiber physiological effect. The benefits of galactooligosaccharides ingestion arise from a population of bifidobacteria in the colon that suppress the activity of putrefactive bacteria and reduce the formation of toxic fermentation products. Scopulariopsis strains showed good productivity of β-galactosidase when grown in a semi-solid fermentation medium. The aim of this work was to extract the β-galactosidase from Scopulariopsis sp. and to evaluate the

  20. Aclimatização de orquídea (Cymbidium sp. em resposta à aplicação de ácidos húmicos

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    Lílian Estrela Borges Baldotto

    2014-05-01

    Full Text Available A aclimatização de mudas de orquídeas propagadas in vitro é um processo lento e que pode ser beneficiado com o uso de bioestimulantes, como, por exemplo, as substâncias húmicas. O presente trabalho objetivou estudar o crescimento de Cymbidium sp. em resposta à aplicação de ácidos húmicos extraídos de compostos orgânicos de cama de frango (AHcf e de esterco bovino (AHeb durante o período de aclimatização. As plantas micropropagadas foram imersas nas soluções de 0, 10, 20, 30 e 40mmol L-1 de C na forma de AHcf e de AHeb e aclimatizadas em viveiro. Aos 150 dias, as plantas foram coletadas para medição das variáveis: número de folhas, altura da planta, diâmetro do caule, matéria fresca da raiz e da parte aérea, matéria seca da raiz, da parte aérea e total, relação entre raiz:parte aérea. Os dados foram submetidos à análise de variância, contraste e análise de regressão. Os resultados mostraram incrementos no acúmulo de matéria seca total das plantas com a aplicação da concentração de 18,75mmol L-1 de C de AHcf e de 3,77mmol L-1 de C de AHeb, com incrementos em relação ao controle de 21 e 28%, respectivamente. Conclui-se que o uso dos ácidos húmicos nas doses indicadas acelera o crescimento das mudas propagadas in vitro de Cymbidium sp., favorecendo a diminuição do período necessário de aclimatização.

  1. Substrate and nutrient solution developed using a biodigestor effluent for melon cultivation

    OpenAIRE

    Villela Jr., Luiz V. E. [UNESP; De Araújo, Jairo A. C. [UNESP; Barbosa, José C. [UNESP; Perez, Luiz R.B.

    2007-01-01

    Buscando a sustentabilidade em uma pequena propriedade agrícola visou-se com o presente estudo, ao aproveitamento do efluente de biodigestor proveniente da fermentação anaeróbia de estrume bovino, no cultivo sem solo do meloeiro. O experimento foi conduzido em Jaboticabal, SP, Brasil, localizado na Latitude 21º 15- 22" S e Longitude 48º 18- 58" W. Cultivou-se o meloeiro (Cucumis melo L. cv. Bonus nº 2) em substrato, com semeadura realizada em outubro de 2003 e se utilizou delineamento experim...

  2. Caracterização da piscicultura na região do Vale do Ribeira - SP Characterization of fish farming in the Ribeira Valley region - SP

    Directory of Open Access Journals (Sweden)

    Daniela Castellani

    2005-02-01

    Full Text Available Este trabalho buscou caracterizar a piscicultura na Região do Vale do Ribeira quanto aos sistemas de manejo. Estudaram-se quarenta e duas pisciculturas sendo que, destas, 36 praticam o sistema semi-intensivo e seis o sistema intensivo, com os seguintes objetivos: engorda de peixes, produção de alevinos e pesque-pagues. Foram listadas 41 espécies de peixes cultivadas. Apenas 6 espécies são nativas da Bacia do Rio Ribeira de Iguape: lambari (Astyanax sp Linneaus, 1758, traíra (Hoplias malabaricus Bloch, 1794, robalo (Centropomus ssp Cuvier e Valenciennes, 1928, jundiá (Rhamdia quelen Quoy e Gaimard, 1824, cascudo (Hypostomus sp Marschall, 1873 e cará (Geophagus brasiliensis Quoy e Gaimard, 1824. Em 95% das pisciculturas foram verificadas fugas de peixes exóticos e alóctones dos cultivos. A tilápia nilótica (Oreochromis niloticus Linneaus, 1758 foi a espécie mais freqüente em escapes, e também é a segunda mais cultivada pelos piscicultores, perdendo somente para o pacu (Piaractus mesopotamicus Halmberg, 1887. Foi possível verificar mediante o cálculo da conversão alimentar, que há um desperdício anual de cerca de 32% da ração utilizada nos cultivos pesquisados. A piscicultura encontra-se em plena expansão nesta região, e já representa a atividade agropecuária mais importante após a bananicultura.The aim of this work was to characterize the fish farming in the Ribeira de Iguape Basin, southern São Paulo State (Brazil, in relation to the management systems. Forty two farms were studied. A semi-intensive system is used by 36 farmers, while an intensive system is used only by 6 studied farmers. Their objectives were raising fish, juvenile fish production and sportive fishery. Forty one fish species were found to be cultivated, but only six were native species from Ribeira Valley: lambari (Astyanax sp Linneaus, 1758, traíra (Hoplias malabaricus Bloch, 1794, robalo (Centropomus ssp Cuvier and Valenciennes, 1928, jundi

  3. Safety evaluation of pectin-derived acidic oligosaccharides (pAOS): genotoxicity and sub-chronic studies.

    NARCIS (Netherlands)

    Garthoff, J.A.; Heemskerk, S.; Hempenius, R.A.; Lina, B.A.; Krul, C.A.; Koeman, J.H.; Speijers, G.J.

    2010-01-01

    Pectin-derived acidic oligosaccharides (pAOS) are non-digestible carbohydrates to be used in infant formulae and medical nutrition. To support its safety, the genotoxic potential of pAOS was evaluated. pAOS was not mutagenic in the Ames test. Positive results were obtained in the chromosome

  4. Safety evaluation of pectin-derived acidic oligosaccharides (pAOS): Genotoxicity and sub-chronic studies

    NARCIS (Netherlands)

    Garthoff, J.A.; Heemskerk, S.; Hempenius, R.A.; Lina, B.A.R.; Krul, C.A.M.; Koeman, J.H.; Speijers, G.J.A.

    2010-01-01

    Pectin-derived acidic oligosaccharides (pAOS) are non-digestible carbohydrates to be used in infant formulae and medical nutrition. To support its safety, the genotoxic potential of pAOS was evaluated. pAOS was not mutagenic in the Ames test. Positive results were obtained in the chromosome

  5. Prevalência e fatores associados ao indicativo de depressão entre idosos residentes na zona rural

    Directory of Open Access Journals (Sweden)

    Pollyana Cristina dos Santos Ferreira

    2013-04-01

    Full Text Available O presente estudo objetivou verificar a prevalência de idosos com indicativo de depressão, segundo sexo e faixa etária, e identificar os fatores associados ao indicativo de depressão. Estudo analítico, transversal e observacional, realizado com 850 idosos residentes na zona rural de um município de Minas Gerais. Para a análise dos dados aplicou-se a fórmula de taxa de prevalência e o modelo de regressão logística (p<0,05. O Projeto foi aprovado pelo Comitê de Ética em Pesquisa com Seres Humanos. A prevalência de indicativo de depressão correspondeu a 22%, com maior ocorrência entre o sexo feminino e na faixa etária entre 60├70 anos. O sexo feminino, o maior número de comorbidades e de incapacidade funcional para o desempenho de atividades instrumentais da vida diária permaneceram associados ao indicativo de depressão. Esses resultados reforçam a necessidade de se implementarem ações de promoção de saúde e prevenção de agravos, com enfoque para a depressão.

  6. Enkapsulasi B. bassiana menggunakan maizena dan daya infeksinya terhadap larva Aedes aegypti, Anopheles sp., Culex sp.

    Directory of Open Access Journals (Sweden)

    Mutiara Widawati

    2015-12-01

    Full Text Available Abstract. Encapsulation formulae of mycoinsecticide have to be able to maintain fungus viability and pathogenicity. This mycoinsecticide was developed as an alternative way to control mosquito borne disease. The aim of this study was to encapsulate Beauveria bassiana as viable storage and have the capability to kill larvae of Aedes aegypti, Anopheles sp. and Culex sp. Mosquito larvae obtained from laboratory reared at health research laboratory; Loka Litbang P2B2 Ciamis. The treatments made in this study were the formu-lation of cornstarch and controls for comparison. This study showed potential formulation of cornstarch encapsulation as a biolarvacidal. Cornstarch formulations proven to be succeed in maintaining fungus viability, however, the pathogenicity of the microcapsule still not effective to kill Aedes, Culex and Anopheles larvae. Keywords: encapsulation, Beauveria bassiana, Ae.aegypti, Anopheles sp., Culex sp. Abstrak. Pembuatan formula bioinsektisida yang optimal sebagai salah satu alternatif untuk pengen-dalian nyamuk vektor perlu di kembangkan. Sediaan mikoinsektisida yang dibuat harus dapat memper-tahankan viabilitas jamur B. bassiana sehingga masih efektif pada saat penggunaannya. Salah satu cara yang digunakan untuk menjaga kestabilan sediaan mikoinsektisida yang berdampak langsung pada via-bilitas jamur adalah dengan menerapkan metode enkapsulasi. Penelitian ini bertujuan untuk meng-hasilkan sediaan mikokapsul dari Beauvaria bassiana melalui proses enkapsulasi menggunakan maizena yang memiliki kapabilitas tinggi sebagai penyimpan B. bassiana dan efektif dalam membunuh larva dan telur Aedes aegypti, Anopheles sp. dan Culex sp. Semua larva uji berasal dari insektarium laboratorium penelitian kesehatan Loka litbang P2B2 Ciamis. Pembuatan enkapsulasi dimulai dengan kultur dan pema-nenan B. bassiana, uji viabilitas, proses enkapsulasi serta uji larvasida di laboratorium. Uji dilakukan dengan satu perlakuan dan satu kontrol untuk

  7. Effectivity of the Earthworms Pheretima hupiensis, Eudrellus sp. and Lumbricus sp. on the Organic Matter Decomposition Process

    Directory of Open Access Journals (Sweden)

    Ea Kosman Anwar

    2009-05-01

    Full Text Available The earthworms are the one of soil fauna component in soil ecosystem have an important role in organic matter decomposition procces. The earthworm feed plant leaf and plant matter up to apart and dissolved. Earthworm metabolisms produce like faeces that mixed with decomposed organic matter mean vermicompost. The vermicompost fertility varies because of some kind of earthworm differ in “niche” and attitude. The experiment was to study the effectivity of earthworm on organic matter decomposition which has been conducted in Soil Biological and Healthy Laboratory and Green House of Soil Research Institute Bogor, during 2006 Budget Year. The three kind of earthworms i.e Pheretima hupiensis, Lumbricus sp. and Eudrellus sp. combined with three kind of organic matter sources i.e rice straw, trash and palm oil plant waste (compost heap. The result shows that the Lumbricus sp. are the most effective decomposer compared to Pheretima hupiensis and Eudrellus sp. and the organic matter decomposed by Lumbricus sp. as followed: market waste was decomposed of 100%, palm oil empty fruit bunch (compost heap 95.8 % and rice straw 84.9%, respectively. Earthworm effectively decreased Fe, Al, Mn, Cu dan Zn.

  8. UJI KUALITATIF DAN KUANTITATIF EKSTRAK Sargassum sp. DAN Gracilaria sp. SEBAGAI INHIBITOR BIO-KOROSI PADA BAJA KARBON

    Directory of Open Access Journals (Sweden)

    Isriyanti Affifah

    2016-07-01

    Full Text Available Korosi atau perkaratan logam merupakan proses oksidasi suatu logam dengan udara atau elektrolit. Udara atau elektrolit tersebut akan mengalami reduksi, sehingga proses korosi merupakan proses elektrokimia. Pada penelitian sebelumnya diketahui bahwa korosi yang disebabkan mikroorganisme pengoksidasi besi (Thiobacillus ferooxidans memiliki peranan yang cukup signifikan terhadap kerugian ekonomi bagi industri. Lapisan biofilm yang dihasilkan mikroorganisme pada permukaan logam dapat mengubah karakteristik elektrokimia permukaan logam tersebut dan dapat menginduksi terjadinya korosi. Untuk mengatasi masalah tersebut, pada penelitian ini dilakukan ekstraksi Sargassum sp. dan Gracilaria sp. yang diduga efektif menginhibisi pertumbuhan mikroba pengoksidasi besi (Thibacillus ferooxidans yang biasanya terdapat di bangunan bawah laut. Hasil ekstraksi Sargassum sp. dan Gracilaria sp. menggunakan pelarut metanol-kloroform (1:1 memberikan yield terhadap berat basah sebesar 44,5% dan 36,5%. Ekstrak tersebut diuji bioaktivitasnya terhadap pertumbuhan T. ferooxidans secara kualitatif (kasat mata dan kuantitatif (metode weight-loss. Melalui kurva pertumbuhan diketahui bahwa T. ferooxidans mampu tumbuh sampai hari ke-7 dan mengalami fasa stasioner pada hari ke-8. Analisis metode weight-loss dilakukan menggunakan coupon dengan luas permukaan 3,6 cm2. Hasil analisis menunjukkan bahwa ekstrak Gracilaria sp mampu menginhibisi 29,3% lebih efektif daripada biocide komersial.

  9. Anatomia comparativa dos nervos da pelve de macacos-prego (Sapajus sp

    Directory of Open Access Journals (Sweden)

    Ediana Vasconcelos da Silva

    2017-01-01

    Full Text Available Macacos-prego (Sapajus sp, inesperadamente, compartilham com chimpanzés comportamentos como alta cognição e memória, uso de ferramentas com o bipedalismo intermitente, tolerância social. No entanto, sua anatomia ainda é pouco estudada. Para verificar a hipótese com qual espécie e/ou grupo de primatas os macacos-prego compartilham mais características, o objetivo deste trabalho foi estudar os nervos pélvicos do Sapajus e compará-los com dados da literatura anatômica sobre os seres humanos, chimpanzés e babuínos, considerando aspectos como origem, trajetória e estruturas inervadas. Foi observado que existem grandes variações nos nervos pélvicos entre os primatas estudados aqui, quais sejam, 1 o problema da posição anatômica, i.e., alguns primatologistas consideram a posição anatômica humana para os primatas, outros consideram a posição anatômica animal, e a opção por um ou outro não é clara nos textos; 2 o problema dos membros pélvicos em primatas não humanos serem lateralizados e semi-fletidos em relação aos seres humanos modernos; 3 o problema da ausência, nos seres humanos modernos, de alguns músculos da coxa em relação aos outros primatas como o escansório e o iliosquiofemoral; e 4] o problema da diferença do número de vértebras nos primatas estudados aqui, inclusive com diferenças para a mesma espécie citadas por diferentes autores tanto para chimpanzés como para macacos-prego.

  10. Biosorption of chromium by mangrove-derived Aplanochytrium sp ...

    African Journals Online (AJOL)

    The microbial dried biomass of Thraustochytrids is used as bioadsorbent for the removal of the chromium in aqueous solution. In this investigation, three species of Thraustochydrids namely Aplanochytrium sp., Thraustochytrium sp. and Schizochytrium sp. were tested for the efficiency of chromium accumulation by culturing ...

  11. A new p-hydroxybenzoic acid derivative from an endophytic fungus Penicillium sp. of Nerium indicum.

    Science.gov (United States)

    Ma, Yang-Min; Qiao, Ke; Kong, Yang; Guo, Lin-Xin; Li, Meng-Yun; Fan, Chao

    2017-12-01

    A new p-hydroxybenzoic acid derivative named 4-(2'R, 4'-dihydroxybutoxy) benzoic acid (1) was isolated from the fermentation of Penicillium sp. R22 in Nerium indicum. The structure was elucidated by means of spectroscopic (HR-ESI-MS, NMR, IR, UV) and X-ray crystallographic methods. The antibacterial and antifungal activity of compound 1 was tested, and the results showed that compound 1 revealed potent antifungal activity against Colletotrichum gloeosporioides, Alternaria alternata, and Alteranria brassicae with MIC value of 31.2 μg/ml.

  12. Trichoderma sp. dalam Pengendalian Penyakit Layu Fusarium pada Tanaman Tomat

    OpenAIRE

    Novita, Trias

    2013-01-01

    Penelitian ini bertujuan untuk mengetahui peran Trichoderma sp dalam pengendalianpenyakit layu fusarium pada tanaman tomat. Penelitian dilaksanakan di Rumah Kaca FakultasPertanian Universitas Jambi, perlakuannya terdiri dari : t0 = tanpa Trichoderma sp; t1 = 25 gTrichoderma sp/8 kg media; t2 = 50 g Trichoderma sp/8 kg media; t3 = 75 g Trichoderma sp/8 kgmedia; dan t4 = 100 g Trichoderma sp /8 kg media. Hasil penelitian menunjukkan bahwa Trichodermasp berperan dalam mengendalikan penyakit layu...

  13. Penggunaan Jamur Antagonis Trichoderma sp. dan Gliocladium sp. untuk Mengendalikan Penyakit Layu (Fusarium oxysporum) pada Tanaman Bawang Merah (Allium ascalonicum L.)

    OpenAIRE

    Ramadhina, Arie

    2015-01-01

    Arie Ramadhina, 2012. The Use of Antagonism Fungus of Trichoderma sp and Gliocladium sp. for Controlling Wilt (Fusarium oxysporum) in Red Onion Plants (Allium ascolanicum). Supervised by Lisnawita and Lahmuddin Lubis. The aim of the research was to know the effectiveness of antagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants. The research was performed in the green-house at the faculty of Agriculture, USU, from February until May, 2012. The researc...

  14. Intoxicação experimental em coelhos por Mascagnia sp (Malpighiaceae procedente do Estado de Santa Catarina Experimental poisoning in rabbits by Mascagnia sp (Malpighiaceae collected in the State of Santa Catarina, southern Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Hubinger Tokarnia

    1998-04-01

    Full Text Available As folhas novas ou a brotação e as folhas maduras de Mascagnia sp, arbusto escandente da família Malpighiaceae, procedente de Santa Catarina, planta comprovadamente tóxica a bovinos, foram administradas sob forma de pó suspenso em água por sonda gástrica, até poucas semanas após a sua colheita, a 45 coelhos adultos. A planta colhida em três municípios do Estado de Santa Catarina, dessecada na sombra a temperatura ambiente, demonstrou possuir toxidez também para essa espécie animal. Porém a sua toxidez para coelhos foi muito variável. Os experimentos realizados não permitem concluir que a procedência da planta, o seu estado de evolução ou a época do ano da colheita, sejam fatores que influenciam a sua toxidez. Já em relação ao quadro clínico, os achados de necropsia e histopatológicos, a planta teve um comportamento muito constante. Os coelhos mostraram os primeiros sintomas de intoxicação entre 3 horas e 24h49min após o começo da administração da planta. A evolução do quadro clínico foi de 1 a 4 minutos. Os sintomas consistiram sempre em que o animal subitamente começava a fazer movimentos desordenados, geralmente violentos; finalmente ficava caído, tinha respiração dispnéica com movimentos respiratórios cada vez mais espaçados, dava alguns gritos e logo morria. Os achados de necropsia se resumiram em congestão hepática e esplenomegalia; o fígado ao corte tinha a lobulação nítida, às vezes com o centro dos lóbulos mais claro. Os exames histopatológicos revelaram alterações regressivas e circulatórias no fígado, rim e baço. A planta moída guardada em vidros hermeticamente fechados com tampa plástica e à temperatura ambiente, submetida a experimentos em outros 22 coelhos 1 ano ou mais após sua colheita, demonstrou ter perdido variavelmente em toxidez.Freshly dried and ground young leaves or sprouts and mature leaves of Mascagnia sp, a creeping shrub of the Malpighiaceae family from Santa

  15. Programmēšanas spēle "PyDrone"

    OpenAIRE

    Siliņš, Viesturs

    2010-01-01

    Darbā “Programmēšanas spēle “PyDrone”” ir dokumentēta atvērtā koda programmas PyDrone izstrāde. PyDrone ir programmēšanas spēle, kas paredzēta izklaidei un izglītošanai, spēles veidā iepazīstinot cilvēkus ar programmēšanu. Programma izstrādāta, izmantojot Python programmēšanas valodu. Programma izstrādāta, izmantojot spējo metodiku programmizstrādes principus. Atslēgvārdi: Python, spējās metodikas, programmēšanas spēle.

  16. Associacao entre exposicao ao material particulado e internacoes por doencas respiratorias em criancas

    Directory of Open Access Journals (Sweden)

    Ana Cristina Gobbo Cesar

    2013-12-01

    Full Text Available O objetivo desse estudo foi estimar a associação entre exposição a material particulado com menos de 2,5 micra de diâmetro aerodinâmico e internações por doenças respiratórias em crianças. Foi realizado estudo ecológico de séries temporais com indicadores diários de internação por doenças respiratórias, em crianças de zero a dez anos de idade, residentes em Piracicaba, SP, entre 1º de agosto de 2011 e 31 de julho de 2012. Utilizou-se modelo aditivo generalizado da regressão de Poisson. Os riscos relativos foram RR = 1,008; IC95% 1,001;1,016 para o lag 1 e RR = 1,009; IC95% 1,001;1,017 para o lag 3. O incremento de 10 μg/m 3 de material particulado com menos de 2,5 micra de diâmetro implicou aumento no risco relativo entre 7,9 e 8,6 pontos percentuais. Concluiu-se que a exposição ao material particulado com menos de 2,5 micra de diâmetro aerodinâmico esteve associada às internações por doenças respiratórias em crianças.

  17. Babesia peircei sp. nov. from the jackass penguin

    African Journals Online (AJOL)

    1992-01-09

    Jan 9, 1992 ... An avian piroplasm, Babesia peircei sp. nov. is described from the jackass penguin Spheniscus demersus. Morphological differences between Babesia peircei sp. nov. and the other valid Babesia spp. are discussed together with the possible vectors. 'n Voal-piroplasma, Babesia peircei sp. nov. afkomstig ...

  18. Pertumbuhan Chlorella sp. pada beberapa konsentrasi limbah batubara (The growth rate of the Chlorella sp. at different concentrations of coal waste water

    Directory of Open Access Journals (Sweden)

    Zerli Selvika

    2016-12-01

    Full Text Available Chlorella sp. is a single-celled microalga that mostly grows in marine waters. Chlorella sp. can grow in heavy polluted waters and therefore it has potency as a bioremediation agent. This study aimed was to analyze the effect of coal on the growth of Chlorella sp. in plant isolation media and the quality of water in plant isolation media for Chlorella sp. The complete randomized design with 4 treatments of coal concentration was used in this study. Four concentration concentrations were tested namely, 0 ppt, 1 ppt, 3 ppt and 5 ppt. The results revealed that coal with different concentrations gave no significant effect on the growth of Chlorella sp. (p> 0.05. The density among the concentrations of 0 ppt, 1 ppt, 3 ppt and 5 ppt were not significantly different. In addition, the coal concentration gave no significant effect on temperature, salinity and potential hydrogen (pH (p>0.05. The Chlorella sp. can grow in the polluted water by coal, and therefore this alga can be used as potential organisms for bioremediation of coal waste. Chlorella sp. merupakan mikroalga bersel satu yang banyak tumbuh di perairan laut. Chlorella sp. dapat tumbuh di perairan yang tercemar berat sehingga berpotensi sebagai bioremediator. Penelitian ini bertujuan untuk menganalisis pengaruh konsentrasi batubara terhadap pertumbuhan Chlorella sp. dan kualitas air pada media kultur Chlorella sp. Metode yang digunakan dalam penelitian ini adalah metode eksperimen skala laboratorium. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 4 perlakuan konsentrasi batubara 0 ppt, 1 ppt, 3 ppt dan 5 ppt. Hasil penelitian menunjukkan bahwa batubara dengan konsentrasi yang berbeda tidak berpengaruh nyata terhadap laju pertumbuhan Chlorella sp (P>0,05. Kepadatan antara konsentrasi 0 ppt, 1 ppt, 3 ppt dan 5 ppt tidak terlalu jauh berbeda. Konsentrasi batubara juga tidak berpengaruh nyata terhadap parameter suhu, salinitas dan derajat keasaman (pH (p>0,05. Chlorella sp

  19. Prevalência de dor associada ao transporte de material escolar

    Directory of Open Access Journals (Sweden)

    Windsor Ramos da Silva Júnior

    2014-10-01

    Full Text Available http://dx.doi.org/10.5007/1980-0037.2014v16n6p680 O objetivo do trabalho foi verificar a prevalência de dor associada ao transporte de material escolar por estudantes universitários. Realizou-se pesquisa transversal, quantitativa, descritiva-analítica, na qual foram avaliados 373 estudantes universitários da Universidade Estadual da Paraíba, entre fevereiro e setembro de 2012. As informações foram coletadas através de questionário sobre dados sociodemográficos, acadêmicos, transporte do material escolar e presença de dor, sendo ainda realizada a medição de dados antropométricos e pesagem de todos os volumes transportados pelo indivíduo. Os dados foram analisados de forma descritiva e inferencial através do software SPSSÒ 17.0. Utilizou-se modelo de Regressão Logística Hierárquica Binária, pelo método Backward LR, no qual o poder de influência das variáveis preditoras – divididas em blocos – foi testado na presença de dor musculoesquelética. Constatou-se uma prevalência de dor de 82,84%, com média geral de 5,21 pontos na Escala Visual Analógica (EVA para avaliação de dor. Nas mulheres, a influência exercida pela massa relativa dos volumes sobre a presença de dor foi 45,1% maior que entre os homens (OR = 0,689; IC95% = 0,503 – 0,942 para cada 1% de incremento. O tempo de carga, por sua vez, aumentou em 22,9% a probabilidade da presença de dor, a cada 15 minutos decorridos (OR = 1,229; IC95% = 1,090 – 1,386. Verificou-se alta prevalência de dor relacionada ao transporte de material escolar e a influência preditora de variáveis como peso relativo da carga transportada e tempo de transporte desse material, especialmente nos indivíduos do sexo feminino.

  20. Cdc20 mediates D-box-dependent degradation of Sp100

    International Nuclear Information System (INIS)

    Wang, Ran; Li, Ke-min; Zhou, Cai-hong; Xue, Jing-lun; Ji, Chao-neng; Chen, Jin-zhong

    2011-01-01

    Highlights: ► Cdc20 is a co-activator of APC/C complex. ► Cdc20 recruits Sp100 and mediates its degradation. ► The D-box of Sp100 is required for Cdc20-mediated degradation. ► Sp100 expresses consistently at both the mRNA and protein levels in cell cycle. -- Abstract: Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Sp100 is a PML-NB scaffold protein, which localizes to nuclear particles during interphase and disperses from them during mitosis, participates in viral resistance, transcriptional regulation, and apoptosis. However, its metabolism during the cell cycle has not yet been fully characterized. We found a putative D-box in Sp100 using the Eukaryotic Linear Motif (ELM) predictor database. The putative D-box of Sp100 was verified by mutational analysis. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Only an overexpressed D-box deletion mutant of Sp100 accumulated in HEK293 cells that also overexpressed Cdc20. Cdc20 knockdown by cdc20 specific siRNA resulted in increased Sp100 protein levels in cells. Furthermore, we discovered that the Cdc20 mediated degradation of Sp100 is diminished by the proteasome inhibitor MG132, which suggests that the ubiquitination pathway is involved in this process. However, unlike the other Cdc20 substrates, which display oscillating protein levels, the level of Sp100 protein remains constant throughout the cell cycle. Additionally, both overexpression and knockdown of endogenous Sp100 had no effect on the cell cycle. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process. These findings expand our knowledge of both Sp100 and Cdc20 as well as their role in ubiquitination.

  1. Condicionantes sociodemográficos e sexuais do conhecimento, atitude e prática de presidiárias quanto ao uso de preservativos

    OpenAIRE

    Nicolau, Ana Izabel Oliveira; Pinheiro, Ana Karina Bezerra

    2012-01-01

    Objetivou-se avaliar o conhecimento, a atitude e a prática de presidiárias quanto ao uso do preservativo masculino e feminino, como medida preventiva às DSTs/HIV e suas relações com aspectos sociodemográficos e sexuais. Pesquisa quantitativa, avaliativa, do tipo Inquérito Conhecimento, Atitude e Prática, envolvendo 155 presidiárias. A coleta de dados realizou-se de janeiro a março de 2010 na penitenciária feminina do Estado do Ceará. Das mulheres investigadas, 35 (22,6%) tinham conhecimento a...

  2. TurboSP and the Topological Trigger

    CERN Document Server

    Belavin, Vladislav

    2016-01-01

    TurboSP was originally proposed as an alternative to Full stream in LHCb data flow. TurboSP is a data flow strategy which not only selects events that should be preserved, like in Full stream, but also provides selective persistence. This is achieved by saving candidates and subset of the reconstruction. During this summer project we investigated the physics viability of using TurboSP with the topological lines and found out a possibility to reduce the number of kept tracks per event by two times while keeping a ratio of fully picked up interesting decay modes on $\\sim 97 \\%$ level.

  3. Lisina digestível para frangos de corte machos entre 12 e 22 dias de idade

    Directory of Open Access Journals (Sweden)

    P Takeara

    2010-12-01

    Full Text Available Avaliou-se nível de lisina digestível para 1050 frangos de corte dos 12 aos 22 dias de idade. O delineamento experimental foi inteiramente ao acaso, com cinco tratamentos, sete repetições e 30 aves por unidade experimental. Os tratamentos foram: 1,05; 1,10; 1,15; 1,20 e 1,25% de lisina digestível. Avaliaram-se ganho de peso, consumo de ração, conversão alimentar, composição e deposição de nutrientes corporais. Foram constatados efeitos quadráticos de lisina digestível no consumo de ração e resposta linear ascendente no peso da carcaça. Na composição química da carcaça, houve resposta quadrática do nível de lisina na concentração de proteína. As taxas de deposição proteica, deposição de água, da carcaça e do corpo total tiveram aumento linear em resposta ao acréscimo de lisina na dieta. O aumento da concentração de lisina, todavia, coincidiu com a redução da matéria mineral nas vísceras e sangue e no corpo total. Considerado o desempenho, o nível 1,1% de lisina digestível atendeu às necessidades do frango de corte entre o 12º e o 22º dia de idade. Consideradas a composição química e as taxas de deposição dos nutrientes corporais, a demanda pelo aminoácido digestível torna-se igual ou maior que 1,25%

  4. Interaction of uranium with Pleurotus sp

    International Nuclear Information System (INIS)

    Ohnuki, Toshihiko; Sakamoto, Fuminori; Kozaki, Naofumi; Ozaki, Takuro; Samadfam, Mohammad

    2002-01-01

    Uptake of uranium by higher fungi, such as mushroom is little elucidated. We have studied the interaction of uranium with Pleurotus sp. (a mushroom) in pure culture over a wide range of U concentration (50-3000 mg/L). The Pleurotus sp. was cultured in two different media. One was rice bran medium, and the other was agar (yeast extract, peptone and dextrose) medium. The uptake of uranium in Pleurotus sp. was examined by alpha ray autoradiography (A,A), X-ray fluorescence spectroscopy (XRF) and scanning microcopy (SEM) equipped with EDS. In the agar medium, the higher uranium concentration gave lower growth of mycelia, and no fruiting body was observed. In the rice bran medium, the fruiting body was grown at U concentrations up to 1000 mg/L. The AA and XRF analysis showed that uranium taken up in the fruiting body was below the detection limit. The SEM-EDS analysis indicated that U was distributed in the limited region and was not transported to the mycelia far from U containing medium. It is concluded that uranium affects the growth of Pleurotus sp., and little uranium is taken up by Pleurotus sp. during the growth of both mycelia and fruiting body. (author)

  5. Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans against Vibrio sp. Isolated From Mud Crab (Scylla sp.

    Directory of Open Access Journals (Sweden)

    Imra Imra

    2016-12-01

    Full Text Available Nipah (Nypa fruticans is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 µg/mL than nipah’s fruit extract (415 µg/mL. This activity to be classified to the strong antioxidant activity (IC50<50 µg/mL. The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 µg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.

  6. Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans against Vibrio sp. Isolated From Mud Crab (Scylla sp.

    Directory of Open Access Journals (Sweden)

    Imra Imra

    2017-02-01

    Full Text Available AbstractNipah (Nypa fruticans is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 μg/mL than nipah’s fruit extract (415 μg/mL. This activity to be classified to the strong antioxidant activity (IC50<50 μg/mL. The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 μg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.

  7. Microfibril-associated Protein 4 Binds to Surfactant Protein A (SP-A) and Colocalizes with SP-A in the Extracellular Matrix of the Lung

    DEFF Research Database (Denmark)

    Schlosser, Anders; Thomsen, Theresa H.; Shipley, J. Michael

    2006-01-01

    for phagocytes. Here we describe the molecular interaction between the extracellular matrix protein microfibril-associated protein 4 (MFAP4) and SP-A. MFAP4 is a collagen-binding molecule containing a C-terminal fibrinogen-like domain and a N-terminal located integrin-binding motif. We produced recombinant MFAP4......-A composed of the neck region and carbohydrate recognition domain of SP-A indicating that the interaction between MFAP4 and SP-A is mediated via the collagen domain of SP-A. Monoclonal antibodies directed against MFAP4 and SP-A were used for immunohistochemical analysis, which demonstrates that the two...... molecules colocalize both on the elastic fibres in the interalveolar septum and in elastic lamina of pulmonary arteries of chronically inflamed lung tissue. We conclude, that MFAP4 interacts with SP-A via the collagen region in vitro, and that MFAP4 and SP-A colocates in different lung compartments...

  8. Introduction to the SP theory of intelligence

    OpenAIRE

    Wolff, J Gerard

    2018-01-01

    This article provides a brief introduction to the "Theory of Intelligence" and its realisation in the "SP Computer Model". The overall goal of the SP programme of research, in accordance with long-established principles in science, has been the simplification and integration of observations and concepts across artificial intelligence, mainstream computing, mathematics, and human learning, perception, and cognition. In broad terms, the SP system is a brain-like system that takes in "New" infor...

  9. TRATAMENTO DA CASCA DE QUEIJO CANASTRA COM RESINA E SEUS EFEITOS DURANTE A MATURAÇÃO E NA QUALIDADE COMO FORMA DE MELHORAR O ASPECTO E AGREGAR VALOR AO PRODUTO

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Costa Paiva

    2012-08-01

    aspecto global, cor, textura, consistência e sabor. Quanto às características microbiológicas dos queijos com e sem aplicação de resina na casca, após 15 dias de maturação, a diferença das contagens microbiológicas entre as quatro estações do ano não chegou a um ciclo logarítmico, exceto para mesófilos aeróbios e Staphylococcus aureus. Os coeficientes de variação referentes aos parâmetros microbiológicos dos queijos maturados por 15 dias, em relação às quatro estações do ano, apresentaram-se relativamente baixos, exceto para contagens de Staphylococcus aureus em queijos com e sem aplicação de resina na casca, e para contagem de coliformes totais em queijos sem aplicação de resina na casca. Após 15 dias de maturação, observou-se ausência de Listeria sp. e Salmonella sp. nos queijos com e sem aplicação de resina na casca, nas quatro estações do ano. Nos queijos com aplicação de resina na casca houve menor crescimento de mofos durante a maturação (45 dias em relação àqueles sem aplicação de resina, reduzindo-se as perdas com raspagens ("toalete" dos queijos durante esse período.

  10. Comparative study on the degradation of dibutyl phthalate by two newly isolated Pseudomonas sp. V21b and Comamonas sp. 51F

    Directory of Open Access Journals (Sweden)

    Vinay Kumar

    2017-09-01

    Full Text Available Dibutyl phthalate is (DBP the top priority toxicant responsible for carcinogenicity, teratogenicity and endocrine disruption. This study demonstrates the DBP degradation capability of the two newly isolated bacteria from municipal solid waste leachate samples. The isolated bacteria were designated as Pseudomonas sp. V21b and Comamonas sp. 51F after scanning electron microscopy, transmission electron microscopy, Gram-staining, antibiotic sensitivity tests, biochemical characterization, 16S-rRNA gene identification and phylogenetic studies. They were able to grow on DBP, benzyl butyl phthalate, monobutyl phthalate, diisodecyl phthalate, dioctyl phthalate, and protocatechuate. It was observed that Pseudomonas sp. V21b was more efficient in DBP degradation when compared with Comamonas sp. 51F. It degraded 57% and 76% of the initial DBP in minimal salt medium and in DBP contaminated samples respectively. Kinetics for the effects of DBP concentration on Pseudomonas sp. V21b and Comamonas sp. 51F growth was also evaluated. Stoichiometry for DBP degradation and biomass formation were compared for both the isolates. Two major metabolites diethyl phthalate and monobutyl phthalates were identified using GC–MS in the extracts. Key genes were amplified from the genomes of Pseudomonas sp. V21b and Comamonas sp. 51F. DBP degradation pathway was also proposed.

  11. Pilot cultivation of the green alga Monoraphidium sp producing a high content of polyunsaturated fatty acids in a low-temperature environment

    Czech Academy of Sciences Publication Activity Database

    Řezanka, Tomáš; Nedbalová, Linda; Lukavský, Jaromír; Střížek, A.; Sigler, Karel

    2017-01-01

    Roč. 22, MAR 2017 (2017), s. 160-165 ISSN 2211-9264 R&D Projects: GA ČR GA14-00227S; GA TA ČR TE01020080 Institutional support: RVO:61388971 ; RVO:67985939 Keywords : Monoraphidium sp. * Microalgae * Pilot cultivation Subject RIV: EE - Microbiology, Virology; EF - Botanics (BU-J) OBOR OECD: Microbiology; Plant sciences, botany (BU-J) Impact factor: 3.994, year: 2016

  12. Sp1/Sp3 and DNA-methylation contribute to basal transcriptional activation of human podoplanin in MG63 versus Saos-2 osteoblastic cells

    Directory of Open Access Journals (Sweden)

    Puri Christina

    2007-03-01

    Full Text Available Abstract Background Podoplanin is a membrane mucin that, among a series of tissues, is expressed on late osteoblasts and osteocytes. Since recent findings have focussed on podoplanin's potential role as a tumour progression factor, we aimed at identifying regulatory elements conferring PDPN promoter activity. Here, we characterized the molecular mechanism controlling basal PDPN transcription in human osteoblast-like MG63 versus Saos-2 cells. Results We cloned and sequenced 2056 nucleotides from the 5'-flanking region of the PDPN gene and a computational search revealed that the TATA and CAAT box-lacking promoter possesses features of a growth-related gene, such as a GC-rich 5' region and the presence of multiple putative Sp1, AP-4 and NF-1 sites. Reporter gene assays demonstrated a functional promoter in MG63 cells exhibiting 30-fold more activity than in Saos-2 cells. In vitro DNase I footprinting revealed eight protected regions flanked by DNaseI hypersensitive sites within the region bp -728 to -39 present in MG63, but not in Saos-2 cells. Among these regions, mutation and supershift electrophoretic mobility shift assays (EMSA identified four Sp1/Sp3 binding sites and two binding sites for yet unknown transcription factors. Deletion studies demonstrated the functional importance of two Sp1/Sp3 sites for PDPN promoter activity. Overexpression of Sp1 and Sp3 independently increased the stimulatory effect of the promoter and podoplanin mRNA levels in MG63 and Saos-2 cells. In SL2 cells, Sp3 functioned as a repressor, while Sp1 and Sp3 acted positively synergistic. Weak PDPN promoter activity of Saos-2 cells correlated with low Sp1/Sp3 nuclear levels, which was confirmed by Sp1/Sp3 chromatin immunoprecipitations in vivo. Moreover, methylation-sensitive Southern blot analyses and bisulfite sequencing detected strong methylation of CpG sites upstream of bp -464 in MG63 cells, but hypomethylation of these sites in Saos-2 cells. Concomitantly

  13. Sp(2) covariant quantisation of general gauge theories

    Energy Technology Data Exchange (ETDEWEB)

    Vazquez-Bello, J L

    1994-11-01

    The Sp(2) covariant quantization of gauge theories is studied. The geometrical interpretation of gauge theories in terms of quasi principal fibre bundles Q(M{sub s}, G{sub s}) is reviewed. It is then described the Sp(2) algebra of ordinary Yang-Mills theory. A consistent formulation of covariant Lagrangian quantisation for general gauge theories based on Sp(2) BRST symmetry is established. The original N = 1, ten dimensional superparticle is considered as an example of infinitely reducible gauge algebras, and given explicitly its Sp(2) BRST invariant action. (author). 18 refs.

  14. Sp(2) covariant quantisation of general gauge theories

    International Nuclear Information System (INIS)

    Vazquez-Bello, J.L.

    1994-11-01

    The Sp(2) covariant quantization of gauge theories is studied. The geometrical interpretation of gauge theories in terms of quasi principal fibre bundles Q(M s , G s ) is reviewed. It is then described the Sp(2) algebra of ordinary Yang-Mills theory. A consistent formulation of covariant Lagrangian quantisation for general gauge theories based on Sp(2) BRST symmetry is established. The original N = 1, ten dimensional superparticle is considered as an example of infinitely reducible gauge algebras, and given explicitly its Sp(2) BRST invariant action. (author). 18 refs

  15. Avaliação da atividade antifúngica de extratos de gengibre e eucalipto in vitro e em fibras de bananeira infectadas com Helminthosporium sp = Evaluation of antifungal activity in vitro of ginger and eucalypt extracts on banana fibers infected with Helminthosporium sp

    Directory of Open Access Journals (Sweden)

    Edvirgem Rodrigues

    2006-01-01

    Full Text Available O presente trabalho teve como objetivo avaliar a fungitoxicidade dos extratos brutos aquosos (EBA de gengibre (Zingiber officinalis e eucalipto (Corymbia citriodora sobre o fungo Helminthosporium sp. Este patógeno foi identificado nas fibras do pseudocaule da bananeira, as quais são utilizadas na fabricação de artesanato local. Foram avaliados o crescimento micelial e esporulação do fungo in vitro, além do crescimento da colônia fúngica nas fibras tratadas com os extratos das plantas. As fibras receberam os seguintes tratamentos:preventivo (P, curativo (C e T0, sendo que neste último, a inoculação e o tratamento com os EBAs foram realizados simultaneamente. Os resultados in vitro mostraram que, dos extratostestados, eucalipto foi mais eficiente na redução do crescimento micelial e produção de esporos do fungo. Com relação ao tratamento in vivo, verificou se que apenas as fibras tratadas preventivamente com o EBA de eucalipto não foram afetadas por Helminthosporium sp.The present work aimed to evaluate the fungitoxicity of ginger (Zingiber officinalis and eucalypt’s (Corymbia citriodora aqueouscrude extracts (ACE concerning the Helminthosporium sp fungus. This pathogen was identified in the fibers of banana pseudostem, used in local handicraft production. The fungus mycelial growth and sporulation were evaluated in vitro, as well as the growth of funguscolony on banana fibers treated with these plant extracts. The fibers received the following treatments: preventative, curative and T0 (the inoculation process and the treatments with the ACEs were used at the same time. The in vitro results showed that the eucalypt crude extract was the most efficient in mycelial growth and in reduction of spores production. As for the in vivo treatment, it was observed that fibers, preventatively treated with eucalypt ACE, were not affected by Helminthosporium sp.

  16. Optisk scanning af spørgeskemaer

    DEFF Research Database (Denmark)

    Paulsen, Aksel

    / 1000 felter= 0.370 (95% CI: 0.160-0.729), (p= 0.020)). Der var ingen statistisk forskel mellem optisk scanning (fejl/ 1000 felter= 0.046 (95% CI: 0.001-0.258)) og manuel dobbelt indtastning (p=1.000). Konklusioner: Optisk scanning er et godt alternativ til manuel dobbelt indtastning for spørgeskema med......Formål og baggrund: Patient rapporterede outcomes i form af spørgeskemaer bruges i øgende grad i sundhedssektoren både i klinisk praksis og forskning. Ofte bruges spørgeskemaer i papirformat. Manuel dobbelt indtastning er defineret som guld standard for overføring af data til et elektronisk format......, men processen er tidkrævende. Optisk scanning af spørgeskemaer med automatisk registrering af svar kan være et alternativ, men videre validering af metoden er nødvendig. Design: 200 patienter blev tilfældigt udvalgt fra en kohorte på 5777 patienter der tidligere havde svaret på to forskellige...

  17. O letramento e a organização da informação digital aliados ao aprendizado ao longo da vida

    Directory of Open Access Journals (Sweden)

    Sonia Boeres

    2018-03-01

    Full Text Available O letramento digital é fruto de pensamento crítico e busca potencializar os métodos de estudo pela tecnologia da informação. A questão de pesquisa aqui tratada é compreender como o letramento digital pode contribuir para o aprendizado ao longo da vida. O objetivo é traçar um paralelo entre o letramento e a informação digital e o aprendizado ao longo da vida. Como metodologia fez-se uma revisão de literatura, nacional e internacional, sobre o processo de ensino e aprendizagem, letramento informacional, digital e aprendizado ao longo da vida. Esta pesquisa é de natureza descritiva e base qualitativa. Os principais resultados observados foram que o letramento digital leva a variadas práticas de leitura e escrita por meio das tecnologias da informação. Estas trazem novas possibilidades para educadores e educandos, profissionais e usuários, transcendendo o simples ato de decodificar sinais ou pesquisar na Internet. Ser letrado digitalmente leva à compreensão de que a tecnologia da informação vai além de novas formas de acesso à informação, mas também a novos processos cognitivos e formas de conhecimento. Chegando-se a esse patamar, cabe o árduo e prazeroso trabalho de manter-se atualizado ao longo do tempo, por meio de um esforço pessoal, que envolvem cursos e treinamentos como fonte de aprendizagem e desenvolvimento de trabalho em conjunto com especialistas. Em suma, há que se manter atualizado, acompanhando a evolução tecnológica, e para tanto, ser destemido das tecnologias e do uso das mídias digitais.

  18. SHEAR STRENGTH IN THE GLUE LINE OF Eucalyptus sp. AND Pinus sp.WOOD

    Directory of Open Access Journals (Sweden)

    Juliana Jerásio Bianche

    Full Text Available ABSTRACT To evaluate the adhesive efficiency on the union of glued joints in a particular temperature and humidity conditions for a specified time the adhesive must be submitted to specific load tests, such as shear in the glue line. The objective of this study was to evaluate the shear strength in the glue line of Eucalyptus sp and Pinus sp.woods. Five adhesives (castor oil, sodium silicate, modified silicate, , PVA and resorcinol-formaldehyde, three weights (150 g/m2, 200 g/m2, and 250 g/m2 and two species (Eucalyptus sp. and Pinus sp. of wood were used. Twelve specimens were obtained from each repetition per treatment, corresponding to 108 specimens that were conditioned at a temperature of 23 ± 1°C and relative humidity of 50 ± 2%. The interaction between the weight and type of adhesive was significant for the shear strength in the glue line of eucalyptus wood. However, no interaction between the weight and the adhesive was found for pinus, only the isolated from the adhesive effect. Chemical bonds originated in the polymerization of resorcinol-formaldehyde adhesives and castor bi-component conferred upon these adhesives the greatest resistance in the glue line. Castor and resorcinol-formaldehyde adhesives showed the highest shear strength values in the line of glue and wood failure. Castor adhesive presented satisfactory performance for bonding of eucalyptus and pine woods.

  19. Caracterización ecológica de lombrices nativas (Pheretima sp., Eudovoscolex sp. y Periscolex sp. bajo diferentes usos del suelo (Guaviare, Colombia Ecological characterization of nature earthworm (Pheretima sp., Eudovoscolex sp. y Periscolex sp. under different soil uses (Guaviare, Colombia

    Directory of Open Access Journals (Sweden)

    Fuentes Baca Anamaría

    1998-12-01

    Full Text Available Se caracterizaron las poblaciones de lombrices de tierr.a de las especies Pheretimasp., Eudovoscolexsp. y Periscolex sp., presentes en un Typic Dystropept del municipio El Retorno (Guaviare, Colombia, bajo ocho tipos de usos (pradera sin pastoreo, pradera con semipastoreo, arreglo silvopastoril, cultivos de chontaduro y cacao socolado, rastrojo de cinco años, rastrojo de segundo crecimiento y bosque, utilizando 10 unidades de muestreo por cobertura y evaluando parámetros de abundancia, densidad y biomasa en relación con algunas características físico-químicas del suelo. Los resultados mostraron diferencias significativas entre las cantidades totales de huevos y adultos en el sistema silvopastoril vs. las praderas y en la pradera con semipastoreo vs. pradera sin pastoreo y de adultos en la cobertura chontaduro (Bactrys gasipaes - kudzu vs. cacao (Theobroma cacao. En varios casos, las respuestas de biomasa y densidad no fueron coincidentes. Mientras que en bosque natural las especies se distribuyen por igual, en las demás coberturas se presentan diferentes patrones de distribución. Se encontraron correlaciones entre biomasa, densidad y abundancia de lombrices con los contenidos de fósforo y magnesio y con la densidad aparente del suelo.
    Earthworm populations of Pheretima sp., Eudovoscolex sp. and Periscolex sp found in Typic Dystropepts from El Retorno (Guaviare, Colombia were evaluated for eight different soil uses. Ten experimental units were used to evaluate abundance, density and biomass, as related to soil physico-chemical characteristics. Results showed significant differences between total amount of eggs and adults under forest pasture combinet use vs grazing, and, grazing plus partially grazing vs non-pasture grazing, and, adults in chontaduro (Bactrys gasipaes - Kudzu vs cacao (Theobroma cacao covering. Biomass and densities answers did not show similar results while in undisturbed forest the species have the same pattern

  20. Kinetics of petroleum oil biodegradation by a consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.

    Directory of Open Access Journals (Sweden)

    L. Kachieng’a

    2017-09-01

    Full Text Available Petroleum oil is a complex mixture of substances, the majority of which are hydrocarbons; the latter represent an extremely important and heterogeneous group of compounds that find their way into water resources by anthropogenic or natural ways. The majority of toxic hydrocarbon components of petroleum are biodegradable, where bioremediation using microbial species has become an integral process for the restoration of oil-polluted areas. In this study, three bioremediation processes, namely natural attenuation, nutrient supplementation by adding glucose and biostimulation by adding Tween® 80, were carried out in various petroleum hydrocarbon concentrations in polluted water media using a consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.. A first-order kinetics model was fitted to the biodegradation data to evaluate the biodegradation rate and to determine the corresponding half-life time. First-order kinetics satisfactorily described the biodegradation of the petroleum-based contaminants under abiotic conditions. The results showed an increase in the percentage removal of petroleum oil at the lower petroleum concentrations and a gradual percentage decrease in removing petroleum oil residues occurred when there was an increase in the initial concentrations of the petroleum oil: 39%, 27%, 22%, 12%, 10% for various petroleum oil concentrations of 50, 100, 150, 200, 250 mg/L, respectively. A similar trend was also observed in the glucose-supplemented culture media where the reduction was 45% and 78% for petroleum concentrations of 250 mg/L and 50 mg/L, respectively. Biodegradation of between 33 and 90% was achieved at a Tween® 80 concentration of between 50 mg/L and 250 mg/L. The degradation rate constants for the natural attenuation process ranged between ≥0 to ≤0.50, ≥0 to ≤0.35, ≥0 to ≤0.25, ≥0 to ≤ 0.14 and ≥ 0 to ≤0.11 for petroleum oil concentrations varying from 50, 100, 150

  1. Melatonin enhances lipid production in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions via a multi-level mechanism.

    Science.gov (United States)

    Zhao, Yongteng; Li, Dafei; Xu, Jun-Wei; Zhao, Peng; Li, Tao; Ma, Huixian; Yu, Xuya

    2018-07-01

    In this study, melatonin (MT) promoted lipid accumulation in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions. The lipid accumulation increased 1.22- and 1.36-fold compared with a nitrogen-starved medium and a normal BG-11 medium, respectively. The maximum lipid content was 51.38%. The reactive oxygen species (ROS) level in the presence of melatonin was lower than that in the control group, likely because of the high antioxidant activities. The application of melatonin upregulated the gibberellin acid (GA) production and rbcL and accD expression levels but downregulated the abscisic acid (ABA) content and pepc expression levels. These findings demonstrated that exogenous melatonin could further improve the lipid production in Monoraphidium sp. QLY-1 by regulating antioxidant systems, signalling molecules, and lipid biosynthesis-related gene expression under nitrogen deficiency conditions. Copyright © 2018 Elsevier Ltd. All rights reserved.

  2. OPERA, an automatic PSF reconstruction software for Shack-Hartmann AO systems: application to Altair

    Science.gov (United States)

    Jolissaint, Laurent; Veran, Jean-Pierre; Marino, Jose

    2004-10-01

    When doing high angular resolution imaging with adaptive optics (AO), it is of crucial importance to have an accurate knowledge of the point spread function associated with each observation. Applications are numerous: image contrast enhancement by deconvolution, improved photometry and astrometry, as well as real time AO performance evaluation. In this paper, we present our work on automatic PSF reconstruction based on control loop data, acquired simultaneously with the observation. This problem has already been solved for curvature AO systems. To adapt this method to another type of WFS, a specific analytical noise propagation model must be established. For the Shack-Hartmann WFS, we are able to derive a very accurate estimate of the noise on each slope measurement, based on the covariances of the WFS CCD pixel values in the corresponding sub-aperture. These covariances can be either derived off-line from telemetry data, or calculated by the AO computer during the acquisition. We present improved methods to determine 1) r0 from the DM drive commands, which includes an estimation of the outer scale L0 2) the contribution of the high spatial frequency component of the turbulent phase, which is not corrected by the AO system and is scaled by r0. This new method has been implemented in an IDL-based software called OPERA (Performance of Adaptive Optics). We have tested OPERA on Altair, the recently commissioned Gemini-North AO system, and present our preliminary results. We also summarize the AO data required to run OPERA on any other AO system.

  3. Biodegradation of hexavalent chromium (Cr+6) in wastewater using Pseudomonas sp. and Bacillus sp. bacterial strains

    Energy Technology Data Exchange (ETDEWEB)

    Qasim, Muhammad [Department of Chemical Engineering, American University of Sharjah (United Arab Emirates)

    2013-07-01

    The recovery of toxic metal compounds is a deep concern in all industries. Hexavalent chromium is particularly worrying because of its toxic influence on human health. In this paper, biodegradation of hexavalent chromium (Cr+6) present in wastewater has been studied using two different bacterial strains; Pseudomonas sp. and Bacillus sp. A chemostat (with and without recycle of cells) with 10 L liquid culture volume was used to study the substrate and the biomass cell concentrations with time. Also, the degree of substrate conversion was studied by the varying the dilution rate as an independent parameter. The dilution rate (ratio of feed flow rate to the culture volume) was varied by varying the feed volumetric rate from 110-170 mL/h for inlet hexavalent chromium concentrations of 70 mg/dm3. The results show that a chemostat with recycle gives a better performance in terms of substrate conversion than a chemostat without a recycle. Moreover, the degree of substrate conversion decreases as the dilution rate is increased. Also, Bacillus sp. was found to give higher conversions compared to pseudomonas sp.

  4. Analisis Fosfor pada Cacing Tanah (Megascolex sp. dan Fridericia sp.) Secara Spektrofotometri Sinar Tampak

    OpenAIRE

    Safira, Cut Shafa

    2015-01-01

    Earthworm is natural resource which can be used for medication due to its highly amount of minerals. One of these minerals is phosphorus. The aim of this research are to identify, determine and know the difference content of phosphorus in Megascolex sp. and Fridericia sp. Qualitative analysis shows positive results with addition of ammonium molybdate 4% and BaCl2 5%. Quantitative analysis was done using visible spectrophotometer with ascorbic acid method, measuring blu-colored molybdenum ...

  5. 22 CFR 171.22 - Appeals.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Appeals. 171.22 Section 171.22 Foreign Relations DEPARTMENT OF STATE ACCESS TO INFORMATION AVAILABILITY OF INFORMATION AND RECORDS TO THE PUBLIC Executive Order 12958 Provisions § 171.22 Appeals. Any denial of a mandatory declassification review request...

  6. Bacillus beijingensis sp. nov. and Bacillus ginsengi sp. nov., isolated from ginseng root.

    Science.gov (United States)

    Qiu, Fubin; Zhang, Xiaoxia; Liu, Lin; Sun, Lei; Schumann, Peter; Song, Wei

    2009-04-01

    Four alkaligenous, moderately halotolerant strains, designated ge09, ge10(T), ge14(T) and ge15, were isolated from the internal tissue of ginseng root and their taxonomic positions were investigated by using a polyphasic approach. Cells of the four strains were Gram-positive-staining, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains ge09 and ge10(T) formed one cluster and strains ge14(T) and ge15 formed another separate cluster within the genus Bacillus. 16S rRNA gene sequence similarities with type strains of other Bacillus species were less than 97 %. Levels of DNA-DNA relatedness among the four strains showed that strains ge09 and ge10(T) and strains ge14(T) and ge15 belonged to two separate species; the mean level of DNA-DNA relatedness between ge10(T) and ge14(T) was only 28.7 %. Their phenotypic and physiological properties supported the view that the two strains represent two different novel species of the genus Bacillus. The DNA G+C contents of strains ge10(T) and ge14(T) were 49.9 and 49.6 mol%, respectively. Strains ge10(T) and ge14(T) showed the peptidoglycan type A4alpha l-Lys-d-Glu. The lipids present in strains ge10(T) and ge14(T) were diphosphatidylglycerol, phosphatidylglycerol, a minor amount of phosphatidylcholine and two unknown phospholipids. Their predominant respiratory quinone was MK-7. The fatty acid profiles of the four novel strains contained large quantities of branched and saturated fatty acids. The predominant cellular fatty acids were iso-C(15 : 0) (42.5 %), anteiso-C(15 : 0) (22.2 %), anteiso-C(17 : 0) (7.3 %) and C(16 : 1)omega7c alcohol (5.7 %) in ge10(T) and iso-C(15 : 0) (50.7 %) and anteiso-C(15 : 0) (20.1 %) in ge14(T). On the basis of their phenotypic properties and phylogenetic distinctiveness, two novel species of the genus Bacillus are proposed, Bacillus beijingensis sp. nov. (type strain ge10(T) =DSM 19037(T) =CGMCC 1.6762(T)) and Bacillus ginsengi sp. nov. (type strain ge14

  7. Cdc20 mediates D-box-dependent degradation of Sp100

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Ran; Li, Ke-min; Zhou, Cai-hong; Xue, Jing-lun [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China); Ji, Chao-neng, E-mail: Chnji@fudan.edu.cn [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China); Chen, Jin-zhong, E-mail: kingbellchen@fudan.edu.cn [State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai (China)

    2011-12-02

    Highlights: Black-Right-Pointing-Pointer Cdc20 is a co-activator of APC/C complex. Black-Right-Pointing-Pointer Cdc20 recruits Sp100 and mediates its degradation. Black-Right-Pointing-Pointer The D-box of Sp100 is required for Cdc20-mediated degradation. Black-Right-Pointing-Pointer Sp100 expresses consistently at both the mRNA and protein levels in cell cycle. -- Abstract: Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Sp100 is a PML-NB scaffold protein, which localizes to nuclear particles during interphase and disperses from them during mitosis, participates in viral resistance, transcriptional regulation, and apoptosis. However, its metabolism during the cell cycle has not yet been fully characterized. We found a putative D-box in Sp100 using the Eukaryotic Linear Motif (ELM) predictor database. The putative D-box of Sp100 was verified by mutational analysis. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Only an overexpressed D-box deletion mutant of Sp100 accumulated in HEK293 cells that also overexpressed Cdc20. Cdc20 knockdown by cdc20 specific siRNA resulted in increased Sp100 protein levels in cells. Furthermore, we discovered that the Cdc20 mediated degradation of Sp100 is diminished by the proteasome inhibitor MG132, which suggests that the ubiquitination pathway is involved in this process. However, unlike the other Cdc20 substrates, which display oscillating protein levels, the level of Sp100 protein remains constant throughout the cell cycle. Additionally, both overexpression and knockdown of endogenous Sp100 had no effect on the cell cycle. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process. These findings expand

  8. Growth kinetics and biodeterioration of polypropylene microplastics by Bacillus sp. and Rhodococcus sp. isolated from mangrove sediment.

    Science.gov (United States)

    Auta, H S; Emenike, C U; Jayanthi, B; Fauziah, S H

    2018-02-01

    Interest in the biodegradation of microplastics is due to their ubiquitous distribution, availability, high persistence in the environment and deleterious impact on marine biota. The present study evaluates the growth response and mechanism of polypropylene (PP) degradation by Bacillus sp. strain 27 and Rhodococcus sp. strain 36 isolated from mangrove sediments upon exposure to PP microplastics. Both bacteria strains were able to utilise PP microplastic for growth as confirmed by the reduction of the polymer mass. The weight loss was 6.4% by Rhodococcus sp. strain 36 and 4.0% by Bacillus sp. strain 27 after 40days of incubation. PP biodegradation was further confirmed using Fourier-transform infrared spectroscopy and scanning electron microscopy analyses, which revealed structural and morphological changes in the PP microplastics with microbial treatment. These analyses showed that the isolates can colonise, modify and utilise PP microplastics as carbon source. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. The dynamics of the fungal aerospores Alternaria sp. and Cladosporium sp. in Parisian atmospheric air, in France

    Science.gov (United States)

    Brezoczki, V. M.

    2016-08-01

    The bioallergens occurring naturally in the atmospheric air are microorganisms, pollen grains, plant seeds, leaf and stem scrap, or their protein molecules. The presence of various airborne fungal spores determines a high allergenic potential for public health. This effect is due to the high number of produced spores, which under favourable meteorological conditions (dry weather and wind) reach the surrounding air. This paper traces the dynamics of two types of fungi, Alternaria sp and Cladosporium sp, fungi which can be found outdoors, in the surrounding air, as well as indoors, inside houses (especially the conidia of Cladosporium sp). The effects of these fungal spores on human health are varies, ranging from seasonal allergies (hay fever, rhinitis, sinusitis etc.) to sever afflictions of the respiratory system, onset of asthma, disfunctionalities of the nervous systems, of the immune system, zymoses etc. The monitoring of the dynamics of the aerospores Alternaria sp and Cladosporium sp was carried out between 2010 and 2013, over a period of 42 weeks during one calendar year, from February to the end of September, in the surrounding air in the French capital, Paris. The regional and global climate and meteorological conditions are directly involved in the occurrence and development of fungi colonies, the transportation and dispersion of fungal spores in the atmospheric air, as well as in the creation of the environment required for the interaction of chemical and biological components in the air. Knowledge of the dynamics of the studied fungal aerospores, coupled with climate and meteorological changes, offers a series of information on the magnitude of the allergenic potential these airborne spores can determine. Legal regulations in this domain set the allergen risk threshold for the Alternaria sp aerospores at 3500 ÷ 7000 spores/m3 air/week, and for the Cladosporium sp aerospores at 56,000 spores/m3 air/week. Besides these regulations there exist a series of

  10. Enhanced photocatalytic activity induced by sp 3 to sp 2 transition of carbon dopants in BiOCl crystals

    KAUST Repository

    Sun, Jianguo

    2017-09-19

    The insufficient light absorption and low quantum efficiency limit the photocatalytic performance of wide bandgap semiconductors. Here, we report a facile strategy to engineer the surface disordered defects of BiOCl nanosheets via carbon doping. The surface defects boost the light absorption and also the quantum yields, as the doped carbon atoms exhibit a transition from sp3 to sp2 hybridization at elevated temperature, corresponding to a change of assembly state from 3D cluster to 2D graphite-like structure. This transition results in an effective charge separation and thus one order of enhancement in photocatalytic activity toward phenol degradation under visible light. The current study opens an avenue to introduce sp3 to sp2 transition of carbon dopants for simultaneous increment of light absorption and quantum efficiency for application in photocatalysis and energy conversion.

  11. Atendimento domiciliar ao idoso: problema ou solução?

    Directory of Open Access Journals (Sweden)

    Ciro Augusto Floriani

    Full Text Available O atendimento domiciliar ao idoso tem se tornado um importante instrumento de assistência nos últimos anos, tanto nos países desenvolvidos quanto nos países em desenvolvimento. Vários aspectos éticos, sociais e operacionais têm sido negligenciados e a literatura nacional é escassa em relação a esta temática. A partir de revisão bibliográfica em atendimento domiciliar, este artigo enfoca, do ponto de vista bioético, os potenciais problemas advindos com a implantação dessa crescente e importante modalidade de atendimento. Conclui ser necessário um maior direcionamento ético na implantação do atendimento domiciliar, com políticas de proteção ao paciente, à família e ao cuidador, visando a aperfeiçoar a qualidade dos programas oferecidos.

  12. Fatores associados a baixa adesao ao tratamento medicamentoso em idosos

    Directory of Open Access Journals (Sweden)

    Noemia Urruth Leao Tavares

    2013-12-01

    Full Text Available OBJETIVO : Analisar fatores associados à baixa adesão ao tratamento medicamentoso em idosos. MÉTODOS : Estudo transversal de base populacional, com amostra representativa de 1.593 indivíduos com 60 anos ou mais, residentes na região urbana de Bagé, RS, em 2008. A amostragem foi realizada em múltiplos estágios. Os dados foram coletados em entrevistas individuais nos domicílios. Analisou-se a associação entre a baixa adesão referida ao tratamento medicamentoso mensurado pelo Brief Medication Questionnaire (BMQ e fatores demográficos, socioeconômicos, comportamentais e de saúde, assistência e prescrição. Foi utilizado modelo de regressão de Poisson para estimar as razões de prevalência bruta e ajustada, os respectivos intervalos de confiança de 95% e p-valor (teste de Wald. RESULTADOS : Cerca de 78,0% dos indivíduos referiram ter usado algum medicamento nos sete dias precedentes à entrevista. Desses, cerca de 1 / 3 foram considerados com baixa adesão ao tratamento. Os fatores significativamente associados à baixa adesão foram: idade (65 a 74 anos, não ter plano de saúde, ter que comprar (totalmente ou em parte os seus medicamentos, ter três ou mais morbidades, possuir incapacidade instrumental para a vida diária e usar três ou mais medicamentos. CONCLUSÕES : A utilização elevada de medicamentos, decorrente da alta prevalência de doenças crônico-degenerativas em idosos, e o acesso ao tratamento devem ser considerados pelos profissionais de saúde para adoção de estratégias que visem diminuir a baixa adesão ao tratamento, aumentando a resolutividade terapêutica e a qualidade de vida desses pacientes.

  13. AVALIAÇÃO DA EFICIÊNCIA DE ENXAGUATÓRIOS BUCAIS SOBRE A INATIVAÇÃO DE STREPTOCOCCUS SP. E CANDIDA ALBICANS IN VITRO

    Directory of Open Access Journals (Sweden)

    Ana Barros de Jesus

    2017-04-01

    Full Text Available A sobrevivência de micro-organismos patogênicos na cavidade bucal depende da habilidade de se aderirem ao dente e sintetizarem placas bacterianas, formadas a partir de resíduos da má higienização. Para evitar a formação das placas bacterianas, além da higienização mecânica, podem ser aplicados enxaguatórios bucais, que inibem o crescimento de patógenos. Ao considerar a grande diversidade de marcas de enxaguatórios bucais no mercado, o presente trabalho visou avaliar quatro das principais marcas, quanto à eficácia in vitro na inibição de Streptococcus sp. e Candida albicans. Para tanto, foram utilizados os princípios ativos Clorexidina 0,12%, Triclosan e Fluoreto de Sódio, Cloreto de Cetilpiridínio e Timol, empregado o método de difusão em ágar, para avaliação da formação do halo de inibição. Os melhores resultados obtidos na inibição dos micro-organismos testados foram Clorexidina 0,12% e Triclosan e Fluoreto de Sódio.

  14. Indirect Manganese Removal by Stenotrophomonas sp. and Lysinibacillus sp. Isolated from Brazilian Mine Water

    Directory of Open Access Journals (Sweden)

    Natália Rocha Barboza

    2015-01-01

    Full Text Available Manganese is a contaminant in the wastewaters produced by Brazilian mining operations, and the removal of the metal is notoriously difficult because of the high stability of the Mn(II ion in aqueous solutions. To explore a biological approach for removing excessive amounts of aqueous Mn(II, we investigated the potential of Mn(II oxidation by both consortium and bacterial isolates from a Brazilian manganese mine. A bacterial consortium was able to remove 99.7% of the Mn(II. A phylogenetic analysis of isolates demonstrated that the predominant microorganisms were members of Stenotrophomonas, Bacillus, and Lysinibacillus genera. Mn(II removal rates between 58.5% and 70.9% were observed for Bacillus sp. and Stenotrophomonas sp. while the Lysinibacillus isolate 13P removes 82.7%. The catalytic oxidation of Mn(II mediated by multicopper oxidase was not properly detected; however, in all of the experiments, a significant increase in the pH of the culture medium was detected. No aggregates inside the cells grown for a week were found by electronic microscopy. Nevertheless, an energy-dispersive X-ray spectroscopy of the isolates revealed the presence of manganese in Stenotrophomonas sp. and Lysinibacillus sp. grown in K medium. These results suggest that members of Stenotrophomonas and Lysinibacillus genera were able to remove Mn(II by a nonenzymatic pathway.

  15. Benz[a]anthracene Biotransformation and Production of Ring Fission Products by Sphingobium sp. Strain KK22

    Science.gov (United States)

    Kunihiro, Marie; Ozeki, Yasuhiro; Nogi, Yuichi; Hamamura, Natsuko

    2013-01-01

    A soil bacterium, designated strain KK22, was isolated from a phenanthrene enrichment culture of a bacterial consortium that grew on diesel fuel, and it was found to biotransform the persistent environmental pollutant and high-molecular-weight polycyclic aromatic hydrocarbon (PAH) benz[a]anthracene. Nearly complete sequencing of the 16S rRNA gene of strain KK22 and phylogenetic analysis revealed that this organism is a new member of the genus Sphingobium. An 8-day time course study that consisted of whole-culture extractions followed by high-performance liquid chromatography (HPLC) analyses with fluorescence detection showed that 80 to 90% biodegradation of 2.5 mg liter−1 benz[a]anthracene had occurred. Biodegradation assays where benz[a]anthracene was supplied in crystalline form (100 mg liter−1) confirmed biodegradation and showed that strain KK22 cells precultured on glucose were equally capable of benz[a]anthracene biotransformation when precultured on glucose plus phenanthrene. Analyses of organic extracts from benz[a]anthracene biodegradation by liquid chromatography negative electrospray ionization tandem mass spectrometry [LC/ESI(−)-MS/MS] revealed 10 products, including two o-hydroxypolyaromatic acids and two hydroxy-naphthoic acids. 1-Hydroxy-2- and 2-hydroxy-3-naphthoic acids were unambiguously identified, and this indicated that oxidation of the benz[a]anthracene molecule occurred via both the linear kata and angular kata ends of the molecule. Other two- and single-aromatic-ring metabolites were also documented, including 3-(2-carboxyvinyl)naphthalene-2-carboxylic acid and salicylic acid, and the proposed pathways for benz[a]anthracene biotransformation by a bacterium were extended. PMID:23686261

  16. Morphology and Phylogeny of a New Species of Anaerobic Ciliate, Trimyema finlayi n. sp., with Endosymbiotic Methanogens.

    Science.gov (United States)

    Lewis, William H; Sendra, Kacper M; Embley, T Martin; Esteban, Genoveva F

    2018-01-01

    Many anaerobic ciliated protozoa contain organelles of mitochondrial ancestry called hydrogenosomes. These organelles generate molecular hydrogen that is consumed by methanogenic Archaea, living in endosymbiosis within many of these ciliates. Here we describe a new species of anaerobic ciliate, Trimyema finlayi n. sp., by using silver impregnation and microscopy to conduct a detailed morphometric analysis. Comparisons with previously published morphological data for this species, as well as the closely related species, Trimyema compressum , demonstrated that despite them being similar, both the mean cell size and the mean number of somatic kineties are lower for T. finlayi than for T. compressum , which suggests that they are distinct species. This was also supported by analysis of the 18S rRNA genes from these ciliates, the sequences of which are 97.5% identical (6 substitutions, 1479 compared bases), and in phylogenetic analyses these sequences grouped with other 18S rRNA genes sequenced from previous isolates of the same respective species. Together these data provide strong evidence that T. finlayi is a novel species of Trimyema , within the class Plagiopylea. Various microscopic techniques demonstrated that T. finlayi n. sp. contains polymorphic endosymbiotic methanogens, and analysis of the endosymbionts' 16S rRNA gene showed that they belong to the genus Methanocorpusculum , which was confirmed using fluorescence in situ hybridization with specific probes. Despite the degree of similarity and close relationship between these ciliates, T. compressum contains endosymbiotic methanogens from a different genus, Methanobrevibacter . In phylogenetic analyses of 16S rRNA genes, the Methanocorpusculum endosymbiont of T. finlayi n. sp. grouped with sequences from Methanomicrobia, including the endosymbiont of an earlier isolate of the same species, ' Trimyema sp.,' which was sampled approximately 22 years earlier, at a distant (∼400 km) geographical location

  17. Morphology and Phylogeny of a New Species of Anaerobic Ciliate, Trimyema finlayi n. sp., with Endosymbiotic Methanogens

    Directory of Open Access Journals (Sweden)

    William H. Lewis

    2018-02-01

    Full Text Available Many anaerobic ciliated protozoa contain organelles of mitochondrial ancestry called hydrogenosomes. These organelles generate molecular hydrogen that is consumed by methanogenic Archaea, living in endosymbiosis within many of these ciliates. Here we describe a new species of anaerobic ciliate, Trimyema finlayi n. sp., by using silver impregnation and microscopy to conduct a detailed morphometric analysis. Comparisons with previously published morphological data for this species, as well as the closely related species, Trimyema compressum, demonstrated that despite them being similar, both the mean cell size and the mean number of somatic kineties are lower for T. finlayi than for T. compressum, which suggests that they are distinct species. This was also supported by analysis of the 18S rRNA genes from these ciliates, the sequences of which are 97.5% identical (6 substitutions, 1479 compared bases, and in phylogenetic analyses these sequences grouped with other 18S rRNA genes sequenced from previous isolates of the same respective species. Together these data provide strong evidence that T. finlayi is a novel species of Trimyema, within the class Plagiopylea. Various microscopic techniques demonstrated that T. finlayi n. sp. contains polymorphic endosymbiotic methanogens, and analysis of the endosymbionts’ 16S rRNA gene showed that they belong to the genus Methanocorpusculum, which was confirmed using fluorescence in situ hybridization with specific probes. Despite the degree of similarity and close relationship between these ciliates, T. compressum contains endosymbiotic methanogens from a different genus, Methanobrevibacter. In phylogenetic analyses of 16S rRNA genes, the Methanocorpusculum endosymbiont of T. finlayi n. sp. grouped with sequences from Methanomicrobia, including the endosymbiont of an earlier isolate of the same species, ‘Trimyema sp.,’ which was sampled approximately 22 years earlier, at a distant (∼400 km

  18. Fermentation of Azolla sp. leaves and the utilization as a feed ingredient of tilapia Oreochromis sp.

    Directory of Open Access Journals (Sweden)

    Nur Bambang Priyo Utomo

    2015-03-01

    Full Text Available ABSTRACTThe objective of this study was to study the effect of incubation period on the nutritional content of Azolla sp. meal fermented by Trichoderma harzianum, and its optimum supplementation level in the feed of tilapia Oreochromis sp. In incubation period treatments, fermentation of Azolla meal was performed in two, six, eight, and ten days (AF2, AF6, AF8, AF10 using Trichoderma harzianum as the fermentor. The fish used in this study was tilapia Oreochromis sp. with an average weight of 10.59±1.29 g. The design of the feeding treatments was repeletting commercial feed with Azolla leaves by with different supplementation levels, i.e. 0% (A/control, 30% (B, 60% (C, and 90% (D. Faecal collection for digestibility measurement was conducted for 15 days and fish growth rate was observed for 40 days. Azolla meal fermented for two days (AF2 showed the best results among the other treatments with a crude fiber decrease of 37.19% and protein increase of 38.65%. The results of this study indicate that fermentation can increase the nutritional quality of Azolla meal and its most optimal supplementation level in the diet of tilapia is 30%.Keywords: crude fiber, Azolla sp., tilapiaABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh lama waktu fermentasi daun mata lele Azolla sp. menggunakan kapang Trichoderma harzianum serta dosis optimal dalam pakan ikan nila Oreochromis sp. Proses fermentasi tepung daun mata lele dilakukan selama dua, enam, delapan, dan sepuluh hari (AF2, AF6, AF8, AF10. Ikan uji pada penelitian ini menggunakan ikan nila Oreochromis sp. dengan bobot rata-rata 10,59±1,29 g yang ditebar sebanyak 6 ekor/akuarium berukuran 50×45×30 cm3. Sebagai pakan perlakuan yakni repeletting daun mata lele dengan pakan komersil pada tingkat suplementasi 0% (A/kontrol, 30% (B, 60% (C, dan 90% (D. Pemeliharaan ikan uji dan pengumpulan data dilakukan dengan mengumpulkan feses ikan untuk uji ketercernaan selama 15 hari dan mengamati pertumbuhan

  19. Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria.

    Science.gov (United States)

    Doronina, Nina V; Trotsenko, Yuri A; Kuznetsov, Boris B; Tourova, Tatjana P; Salkinoja-Salonen, Mirja S

    2002-05-01

    Two aerobic, pink-pigmented, facultatively methylotrophic bacteria, strains F20T and RXM(T), are described taxonomically. On the basis of their phenotypic and genotypic properties, the isolates are proposed as novel species of the genus Methylobacterium, Methylobacterium suomiense sp. nov. (type strain F20T = VKM B-2238T = NCIMB 13778T) and Methylobacterium lusitanum sp. nov. (type strain RXMT = VKM B-2239T = NCIMB 13779T).

  20. Diaphite, a new type of surface with mixed sp{sup 2}-sp{sup 3} hybridization for adsorption and functionalization

    Energy Technology Data Exchange (ETDEWEB)

    Radosinski, Lukasz, E-mail: lukasz.radosinski@pwr.edu.pl [Wroclaw University of Science and Technology, Chemistry Department, Group of Bioprocess and Biomedical Engineering, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw (Poland); Formalik, Filip [Wroclaw University of Science and Technology, Chemistry Department, Group of Bioprocess and Biomedical Engineering, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw (Poland); Olejniczak, Adam [Department of Spectroscopy of Excited States, Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw (Poland); Radosz, Andrzej [Wroclaw University of Science and Technology, Faculty of Fundamental Problems of Technology, Department of Quantum Technologies, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw (Poland)

    2017-05-15

    Highlights: • Recent discoveries show a possibility of photoinduced formation of a structure of mixed sp{sub 2}-sp{sub 3} hybridization out of graphite and graphene–diaphite. • Ab initio and molecular mechanics calculations indicate that the surface exhibits large enhancement of binding energy due to favorable sp{sub 3} like hybridization. • The binding energy varies upon configuration of occupied adsorption sites and the adsorption sites form a regular bed-like matrix. - Abstract: We theoretically study a new carbon phase with mixed sp{sup 2}-sp{sup 3} bond hybridization called diaphite. Using ab initio calculations and the adaptive intermolecular reactive bond order (AIREBO) potential approach, we show that the surface of this structure exhibits enhanced adsorption capabilities. Specifically, using hydrogen as a test adsorbate, we calculate that the chemical binding energy, depending on the configuration of hydrogen atoms, varies from 2.08 to 2.9 eV. Furthermore, the adsorption sites form a regular matrix; thus, we postulate that this new stable carbon phase may be a universal matrix for functionalization.

  1. Diversity and antimicrobial susceptibility of oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican crops.

    Science.gov (United States)

    Rodríguez, C; Wachlin, A; Altendorf, K; García, F; Lipski, A

    2007-12-01

    To ameliorate the identification, evaluate the diversity, and determine the antimicrobial sensitivity of 19 oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican crops. Phenotypical, chemotaxonomical, and molecular data allocated most isolates to the species Sten. maltophilia and Ser. marcescens. The API profiles, antimicrobial resistance patterns (ATB system), and BOX-polymerase chain reaction (PCR) genomic fingerprints of isolates of Stenotrophomonas sp. exhibited a higher degree of heterogeneity than those obtained for the isolates of Serratia sp. The former group of bacteria exhibited multiresistance to antimicrobials. In contrast, isolates of Serratia sp. were sensitive to the majority of the drugs tested. Changes in the results of the antibiograms throughout incubation, which indicate an induction of tolerance, were observed for isolates of both the species. Minimum inhibitory concentration of oxytetracycline, determined using E-test stripes, were rather elevated. The occurrence of two species of opportunistic pathogens in crop-associated materials poses a risk to consumers in the community. The phenotypic and genotypic data presented could support epidemiologist and physicians dealing with infections caused by environmental strains of these taxa.

  2. Isolation of C11 Cyclopentenones from Two Didemnid Species, Lissoclinum sp. and Diplosoma sp.

    Directory of Open Access Journals (Sweden)

    Katsuhiro Ueda

    2009-12-01

    Full Text Available A series of new C11 cyclopentenones 1-7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8-12. The structures of 1-7 were elucidated by spectroscopic analyses. Cytotoxicity of the isolated compounds was evaluated against three human cancer cell lines (HCT116, A431 and A549.

  3. Green Decision Making: Sustainable Transport and Systemic Planning (SP)

    DEFF Research Database (Denmark)

    Leleur, Steen

    and infrastructure assessment and on the methodology and process of systemic planning (SP). SP theory development has interchanged with practical application and testing of the SP approach in a large number of cases. The word systemic in SP indicates that complex planning problems and provision of decision support...... in today’s strategic planning needs a focus on what may be addressed as systemic insights in balance with more conventional, systematically-based findings where causal linkages can be modelled and made use of. In practice this means that SP is based on a study-specific combination of hard (quantitative......) and soft (qualitative) operations research (OR) methods; especially the latter have a function as regards knowledge generation that relates to obtaining systemic insights. Furthermore, SP applies a process that drives group-based learning forward. The group should be formed with the different stakeholder...

  4. A superhard sp3 microporous carbon with direct bandgap

    Science.gov (United States)

    Pan, Yilong; Xie, Chenlong; Xiong, Mei; Ma, Mengdong; Liu, Lingyu; Li, Zihe; Zhang, Shuangshuang; Gao, Guoying; Zhao, Zhisheng; Tian, Yongjun; Xu, Bo; He, Julong

    2017-12-01

    Carbon allotropes with distinct sp, sp2, and sp3 hybridization possess various different properties. Here, a novel all-sp3 hybridized tetragonal carbon, namely the P carbon, was predicted by the evolutionary particle swarm structural search. It demonstrated a low density among all-sp3 carbons, due to the corresponding distinctive microporous structure. P carbon is thermodynamically stable than the known C60 and could be formed through the single-walled carbon nanotubes (SWCNTs) compression. P carbon is a direct bandgap semiconductor displaying a strong and superhard nature. The unique combination of electrical and mechanical properties constitutes P carbon a potential superhard material for semiconductor industrial fields.

  5. The complexity of national identity Construction in Curaçao, Dutch Caribbean

    Directory of Open Access Journals (Sweden)

    Rose Mary Allen

    2010-10-01

    Full Text Available Curaçao, one of the five islands of the Dutch Caribbean federation called the Netherlands Antilles, currently stands at a crossroad of constitutional reform. In the context of these changes at the political level, it is logical that national identities are being redefined and repositioned. The developments in the fashioning of national identities in Curaçao deserve attention locally but also regionally. How do people in Curaçao construct national identities in daily life? How is a national collective constructed on the one hand, and how are differences with other collectivities constructed on the other hand? The historical context of constitutional changes that have occurred over the years is considered as well as an examination of the present discourse on constitutional reform. These identity issues are then placed within a wider Caribbean context.  Resumen: La complejidad de la construcción de la identidad nacional en Curaçao,en el Caribe holandés Curaçao, una de las cinco islas de la federación caribeña neerlandesa llamada Antillas Holandesas, se encuentra hoy en día en una encrucijada de reformas constitucionales. En el contexto de estos cambios a nivel político, es lógico que las identidades nacionales sean redefinidas y reposicionadas. Los desarrollos en la formulación de identidades nacionales en Curaçao merecen atención local, y también regional. ¿Cómo construye la gente en Curaçao identidades nacionales en la vida cotidiana? ¿Cómo, por un lado, se construye el colectivo nacional y, por otro, cómo se construyen las diferencias con otras colectividades? En este análisis se estudia el contexto histórico de los cambios constitucionales que han ocurrido en el curso del tiempo y se realiza igualmente una revisión del actual discurso sobre la reforma constitucional. Estos problemas de identidad son analizados entonces en un contexto caribeño más amplio.

  6. Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

    Directory of Open Access Journals (Sweden)

    Lenni Fitri

    2017-07-01

    Full Text Available Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 µg/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.

  7. Requirements Modeling with the Aspect-oriented User Requirements Notation (AoURN): A Case Study

    Science.gov (United States)

    Mussbacher, Gunter; Amyot, Daniel; Araújo, João; Moreira, Ana

    The User Requirements Notation (URN) is a recent ITU-T standard that supports requirements engineering activities. The Aspect-oriented URN (AoURN) adds aspect-oriented concepts to URN, creating a unified framework that allows for scenario-based, goal-oriented, and aspect-oriented modeling. AoURN is applied to the car crash crisis management system (CCCMS), modeling its functional and non-functional requirements (NFRs). AoURN generally models all use cases, NFRs, and stakeholders as individual concerns and provides general guidelines for concern identification. AoURN handles interactions between concerns, capturing their dependencies and conflicts as well as the resolutions. We present a qualitative comparison of aspect-oriented techniques for scenario-based and goal-oriented requirements engineering. An evaluation carried out based on the metrics adapted from literature and a task-based evaluation suggest that AoURN models are more scalable than URN models and exhibit better modularity, reusability, and maintainability.

  8. Role of Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 as Bioindicators and Immobilizers of Chromium in a Contaminated Natural Environment.

    Science.gov (United States)

    Millach, Laia; Solé, Antoni; Esteve, Isabel

    2015-01-01

    The aim of this work was to study the potential of the two phototrophic microorganisms, both isolated from Ebro Delta microbial mats, to be used as bioindicators and immobilizers of chromium. The results obtained indicated that (i) the Minimum Metal Concentration (MMC) significantly affecting Chlorophyll a intensity in Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 was 0.25 µM and 0.75 µM, respectively, these values being lower than those established by current legislation, and (ii) Scenedesmus sp. DE2009 was able to immobilize chromium externally in extracellular polymeric substances (EPS) and intracellularly in polyphosphate (PP) inclusions. Additionally, this microorganism maintained high viability, including at 500 µM. Based on these results, we postulate that Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 are good chromium-indicators of cytotoxicity and, further, that Scenedesmus sp. DE2009 plays an important role in immobilizing this metal in a contaminated natural environment.

  9. Role of Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 as Bioindicators and Immobilizers of Chromium in a Contaminated Natural Environment

    Directory of Open Access Journals (Sweden)

    Laia Millach

    2015-01-01

    Full Text Available The aim of this work was to study the potential of the two phototrophic microorganisms, both isolated from Ebro Delta microbial mats, to be used as bioindicators and immobilizers of chromium. The results obtained indicated that (i the Minimum Metal Concentration (MMC significantly affecting Chlorophyll a intensity in Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 was 0.25 µM and 0.75 µM, respectively, these values being lower than those established by current legislation, and (ii Scenedesmus sp. DE2009 was able to immobilize chromium externally in extracellular polymeric substances (EPS and intracellularly in polyphosphate (PP inclusions. Additionally, this microorganism maintained high viability, including at 500 µM. Based on these results, we postulate that Geitlerinema sp. DE2011 and Scenedesmus sp. DE2009 are good chromium-indicators of cytotoxicity and, further, that Scenedesmus sp. DE2009 plays an important role in immobilizing this metal in a contaminated natural environment.

  10. De restos a rastros: os sambaquis de Cubatão/SP e a construção de uma pré-história brasileira = From rests to traces: the shell mounds of Cubatão/SP and the building of a brazilian prehistory

    Directory of Open Access Journals (Sweden)

    Calazans, Marília Oliveira

    2013-01-01

    Full Text Available Os sambaquis são um testemunho fundamental da complexidade da ocupação do território sulamericano. Contraditoriamente, nunca ocuparam um papel central nos debates da Arqueologia brasileira, que se dedicou por muitas décadas a estabelecer grandes modelos de Pré-história. Restos acidentais da atividade humana, sítios enigmáticos, monumentos: as diferentes formas de conceber os sambaquis ao longo dos séculos pós-colonização atestam que, da especificidade destes sítios, emergem questões sociais, científicas e institucionais que permeiam toda a história da Arqueologia. Neste artigo, verificaremos a evolução deste debate por meio das pesquisas realizadas nos sambaquis da cidade de Cubatão/SP

  11. [A STUDY OF THE ISOLATED BACTERIOPHAGE ΦAB-SP7 ADSORPTION ON THE CELL SURFACE OF THE AZOSPIRILLUM BRASILENSE SP7].

    Science.gov (United States)

    Guliy, O I; Karavaeva, O A; Velikov, V A; Sokolov, O I; Pavily, S A; Larionova, O S; Burov, A M; Ignatov, O V

    2016-01-01

    The bacteriophage ΦAb-Sp7 was isolated from the cells of the Azospirillum brasilense Sp7. The morphology, size of the gram-negative colonies, and range of lytic activity against other strains and species of the genus Azospirillum was tested. The isolated phage DNA was examined using electrophoretic and restriction analysis, and the size of the genome were established. The electron microscopy. resuIts show that the phage (capsid) has a strand-like form. The electron microscopy study of the bacteriophage ΦAb-Sp7 adsorption on the A. brasilense Sp7 bacterial surface was performed.

  12. Prevalência de asma e fatores associados: estudo de base populacional em São Paulo, SP, 2008-2009

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    Clóvis Arlindo de Sousa

    2012-10-01

    Full Text Available OBJETIVO: Estimar a prevalência de asma em crianças e adolescentes e identificar fatores associados. MÉTODOS: Estudo transversal de base populacional com 1.185 crianças e adolescentes de ambos os sexos de São Paulo, SP, de 2008 a 2009. As informações foram coletadas por meio de entrevistas domiciliares e os participantes foram selecionados a partir de amostragem probabilística, estratificada por sexo e idade, e por conglomerados em dois estágios (setores censitários e domicílios. Foi realizada regressão múltipla de Poisson na análise ajustada entre o desfecho e variáveis sociodemográficas, econômicas, estilo de vida e condições de saúde. RESULTADOS: Dos entrevistados, 9,1% (IC95% 7,0;11,7 referiram asma. Após análise ajustada, identificaram-se os seguintes fatores independentemente associados ao agravo: idade (zero a quatro anos/15 a 19 RP = 3,18 (IC95% 1,20;8,42, idade (cinco a nove anos/15 a 19 RP = 6,37 (IC95% 2,64;15,39, idade (10 a 14 anos/15 a 19 RP = 4,51 (IC95% 1,95;10,40, alergia (sim/não RP = 2,22 (IC95% 1,24;4,00, rinite (sim/não RP = 2,13 (IC95% 1,22;3,73, problemas de saúde nos 15 dias prévios à entrevista (sim/não RP = 1,96 (IC95% 1,23;3,11, número de cômodos no domicílio (1 a 3/4 e mais RP = 1,67 (IC95% 1,05;2,66, e cor da pele (preta e parda/branca RP = 2,00 (IC95% 1,14;3,49. CONCLUSÕES: Os achados do presente estudo apontam a importância da asma associada à presença de rinite e alergia, idade entre cinco e nove anos, cor da pele preta e parda e moradia com menor número de cômodos. Os frequentes problemas de saúde podem ser considerados consequência dessa doença.

  13. Optimal control strategy to reduce the temporal wavefront error in AO systems

    NARCIS (Netherlands)

    Doelman, N.J.; Hinnen, K.J.G.; Stoffelen, F.J.G.; Verhaegen, M.H.

    2004-01-01

    An Adaptive Optics (AO) system for astronomy is analysed from a control point of view. The focus is put on the temporal error. The AO controller is identified as a feedback regulator system, operating in closed-loop with the aim of rejecting wavefront disturbances. Limitations on the performance of

  14. The Ashkenazi Jews of Curaçao, a trading minority

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    Eva Abraham-Van der Mark

    2000-07-01

    Full Text Available First describes the early Sephardi presence in Curaçao, the arrival of the Ashkenazi in the 20th c., and the relations between these 2 groups. Author goes on to discuss the Ashkenazis' economic success and the exodus of the 1980s. She asks whether the success and the exodus can be attributed to the characteristics of the group itself or whether conditions and developments in Curaçao account for economic fortune and the departure of the Ashkenazi.

  15. Quality control of Ling'ao nuclear power station civil construction

    International Nuclear Information System (INIS)

    Lu Buliang; Ye Changyuan

    2001-01-01

    Based on the quality control model adopted during Ling'ao Nuclear Power Station construction, the author briefly introduces quality control process of some main civil construction activities (reinforced concrete, steel liner, steel works and prestressing force) of nuclear power station, and makes some descriptions on non-conformance control of civil works. These quality control processes described come from the concrete practice during civil construction of Ling'ao Nuclear Power Station, and are based on Daya Bay Nuclear Power Station construction experience

  16. Gap junctional communication modulates gene transcription by altering the recruitment of Sp1 and Sp3 to connexin-response elements in osteoblast promoters

    Science.gov (United States)

    Stains, Joseph P.; Lecanda, Fernando; Screen, Joanne; Towler, Dwight A.; Civitelli, Roberto

    2003-01-01

    Loss-of-function mutations of gap junction proteins, connexins, represent a mechanism of disease in a variety of tissues. We have shown that recessive (gene deletion) or dominant (connexin45 overexpression) disruption of connexin43 function results in osteoblast dysfunction and abnormal expression of osteoblast genes, including down-regulation of osteocalcin transcription. To elucidate the molecular mechanisms of gap junction-sensitive transcriptional regulation, we systematically analyzed the rat osteocalcin promoter for sensitivity to gap junctional intercellular communication. We identified an Sp1/Sp3 containing complex that assembles on a minimal element in the -70 to -57 region of the osteocalcin promoter in a gap junction-dependent manner. This CT-rich connexin-response element is necessary and sufficient to confer gap junction sensitivity to the osteocalcin proximal promoter. Repression of osteocalcin transcription occurs as a result of displacement of the stimulatory Sp1 by the inhibitory Sp3 on the promoter when gap junctional communication is perturbed. Modulation of Sp1/Sp3 recruitment also occurs on the collagen Ialpha1 promoter and translates into gap junction-sensitive transcriptional control of collagen Ialpha1 gene expression. Thus, regulation of Sp1/Sp3 recruitment to the promoter may represent a potential general mechanism for transcriptional control of target genes by signals passing through gap junctions.

  17. Consumo de drogas psicoativas por adolescentes escolares de Assis, SP

    Directory of Open Access Journals (Sweden)

    José Luiz Guimarães

    2004-02-01

    Full Text Available Com o objetivo de quantificar o consumo das diferentes drogas psicoativas entre os estudantes da cidade de Assis, SP, e investigar as variáveis relacionadas com seu uso, foi aplicado um questionário que identificava dados sociodemográficos e padrão de uso não-médico de psicotrópicos em 20% dos estudantes das escolas públicas e privadas da cidade. Os maiores índices de consumo para o uso na vida foram os do álcool com 68,9% e o tabaco com 22,7%. As drogas mais utilizadas foram: solventes (10,0%; maconha (6,6%; ansiolíticos (3,8%; anfetamínicos (2,6%; cocaína (1,6% e anticolinérgicos (1,0%.

  18. Desempenho em Pastagens e Características de Carcaça da 16a Progênie dos Rebanhos Nelore, Guzerá e Caracu de Sertãozinho (SP

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    Razook Alexander George

    2002-01-01

    Full Text Available Quarenta e um machos inteiros dos rebanhos selecionados para peso aos 378 dias (P378, nascidos em 1996, foram terminados em pastagens de Panicum Maximum (Jacq., Panicum Maximum (Jaq cv. Tanzania 1 e Brachiaria brizantha (Hoschst Stapf cv. Marandu na Estação Experimental de Zootecnia de Sertãozinho (SP. As amostras, representando a média de P378 em cada rebanho, foram: 11 animais Nelore Seleção (NeS e 10 para cada um dos grupos Nelore Controle (NeC, Guzerá Seleção (GuS e Caracu (Ca. O abate ocorreu aos 824 dias de idade e condição corporal 7,6, em uma escala de 1 a 9. As médias mínimas e máximas ajustadas, para as principais características, considerando-se todos os grupos, foram: ganho de peso médio diário, 406 (NeC e 501 g (NeS; peso de abate (PAB, 446,8 (NeC e 544,3 kg (NeS ; peso de carcaça (PCAR, 249,8 (NeC e 309,7 kg (NeS; rendimento de carcaça (REND, 54,0 (GuS e 56,3% (NeC e NeS. No corte entre a 9feminine e 11feminine costelas : músculo 59,6 (NeC e 65,2% (Ca; gordura, 15,6 (Ca e 21,4% (NeC; osso, 18,9 (NeC e 20,2% (GuS; espessura de gordura (ESPGOR, 2,0 (Ca e 4,2 mm (NeC; área de olho de lombo (AOL, 65,6 (NeC e 71,1 cmsuperscript two (NeS e Ca; força de cisalhamento (FC, 4,5 (Ca e 6,6 kg (GuS e perdas totais no cozimento (PERDAS, 22,5 (NeC e 24,9% (GuS. A seleção para peso provocou, em NeS, maiores PAB e PCAR, sem interferir no REND, na composição da costela, FC e PERDAS na carne. Houve, porém, menor ESPGOR em relação à NeC. Os animais GuS apresentaram PAB e PCAR intermediários, entre NeS e Ca, e menor REND e os Ca maior proporção de músculo na costela e carne com maior maciez em relação ao Zebu.

  19. Gene cloning, overexpression, and characterization of a xylanase from Penicillium sp. CGMCC 1669.

    Science.gov (United States)

    Liu, Wanli; Shi, Pengjun; Chen, Qiang; Yang, Peilong; Wang, Guozeng; Wang, Yaru; Luo, Huiying; Yao, Bin

    2010-09-01

    A xylanase-encoding gene, xyn11F63, was isolated from Penicillium sp. F63 CGMCC1669 using degenerated polymerase chain reaction (PCR) and thermal asymmetric interlaced (TAIL)-PCR techniques. The full-length chromosomal gene consists of 724 bp, including a 73-bp intron, and encodes a 217 amino acid polypeptide. The deduced amino acid sequence of xyn11F63 shows the highest identity of 70% to the xylanase from Penicillium sp. strain 40, which belongs to glycosyl hydrolases family 11. The gene was overexpressed in Pichia pastoris, and its activity in the culture medium reached 516 U ml(-1). After purification to electrophoretic homogeneity, the enzyme showed maximal activity at pH 4.5 and 40 degrees C, was stable at acidic buffers of pH 4.5-9.0, and was resistant to proteases (proteinase K, trypsin, subtilisin A, and alpha-chymotrypsin). The specific activity, K (m), and V (max) for oat spelt xylan substrate was 7,988 U mg(-1), 22.2 mg ml(-1), and 15,105.7 micromol min(-1) mg(-1), respectively. These properties make XYN11F63 a potential economical candidate for use in feed and food industrial applications.

  20. Genome Sequence of the Lager-Brewing Yeast Saccharomyces sp. Strain M14, Used in the High-Gravity Brewing Industry in China.

    Science.gov (United States)

    Liu, Chunfeng; Li, Qi; Niu, Chengtuo; Zheng, Feiyun; Li, Yongxian; Zhao, Yun; Yin, Xiangsheng

    2017-10-26

    Lager-brewing yeasts are mainly used for the production of lager beers. Illumina and PacBio-based sequence analyses revealed an approximate genome size of 22.8 Mb, with a GC content of 38.98%, for the Chinese lager-brewing yeast Saccharomyces sp. strain M14. Based on ab initio prediction, 9,970 coding genes were annotated. Copyright © 2017 Liu et al.

  1. Alterações anatomopatológicas renais em indivíduos com a síndrome da imunodeficiência adquirida

    Directory of Open Access Journals (Sweden)

    Laguna-Torres Victor Alberto

    1998-01-01

    Full Text Available As alterações anatomopatológicas renais foram estudadas em 119 casos de indivíduos com a síndrome da imunodeficiência humana adquirida (SIDA no Hospital Escola da Faculdade de Medicina do Triângulo Mineiro, Uberaba MG. A partir das amostras de rim fixadas em formol, foram confeccionadas lâminas e analisadas ao microscópio de luz. Dos 119 casos estudados, 67 tiveram diagnóstico de nefrite túbulo intersticial (NTI, sendo 18 inespecíficas, 2 xantogranulomatosas e encontrou-se agente infeccioso em 48: fungos em 28 (16 Cryptococcus sp, 9 Histoplasma sp, 1 Candida sp e 2 Paracoccidioides brasiliensis; bactérias em 18 (9 Mycobacterium sp; vírus em 6, Citomegalovírus. Em 43 havia necrose tubular aguda (NTA. Outros diagnósticos foram: nefrocalcinose (15,1% e hialinose arteriolar (22,7%. Encontrou-se também 2 casos com glomeruloesclerose segmentar focal (GESF e um caso de hiperplasia mesangial difusa. Houve predomínio da NTI, que pode ser devido às infecções oportunistas, predominando as fúngicas; a toxicidade por drogas ou ainda devido a possível ação direta do próprio vírus. A necrose tubular aguda (NTA, foi a segunda causa em freqüência, de acometimento renal da amostra. Concluiu-se que o envolvimento renal nos pacientes com SIDA apresenta um espectro variado de processos patológicos, principalmente relacionados com infecções oportunistas, o tratamento e os procedimentos para diagnósticos, e ainda as nefropatias associadas ao vírus da imunodeficiência humana (VIH.

  2. Protozoan parasites of Xiphophorus spp. (Poeciliidae and their relation with water characteristics Parasitos protozoários de Xiphophorus sp. (Poeciliidae e a relação deles com as características da água

    Directory of Open Access Journals (Sweden)

    F. Garcia

    2009-02-01

    Full Text Available This work investigated the relationship between water characteristics and infestation of protozoan parasites, Icthyophthirius multifiliis and Trichodina sp., in swordtails (Xiphophorus helleri and platy (Xiphophorus maculates collected in an ornamental fish farm in São Paulo, Brazil. Fish were monthly collected from ponds and tanks for one year. The prevalence rates in fish from tanks and ponds were, respectively, 34.2% and 22.5% for I. multifiliis, and 13% and 54.2% for Trichodina sp. Increased electric conductivity and pH provoked reduction of I. multifiliis infestation. Low oxygen concentration increased Trichodina sp. infestation. The use of salt to increase the electric conductivity of water was a method to control I. multifiliis. Low dissolved oxygen and the addition of organic fertilizer favored the reproduction of Trichodina sp.Investigou-se a relação entre as características da água e a infestação de protozoários parasitos, Icthyophthirius multifiliis e Trichodina sp., em peixe espada, Xiphophorus helleri e em plati, Xiphophorus maculatus, coletados em uma piscicultura de peixes ornamentais no Estado de São Paulo, Brasil. Os peixes foram coletados mensalmente, durante um ano, dos viveiros e das caixas de estocagem. A prevalência da infestação nos peixes das caixas e dos viveiros foram, respectivamente, 34,2% e 22,5% para I. multifiliis e 13% e 54% para Trichodina sp. A elevada condutividade elétrica e o pH da água reduziram a infestação por I. multifiliis. A baixa concentração de oxigênio resultou em aumento na infestação por Trichodina sp. O uso do sal, para aumentar a condutividade elétrica da água, consistiu em um método de controle de I. multifiliis. A redução do oxigênio dissolvido e a adição de fertilizante orgânico favoreceram a reprodução de Trichodina sp.

  3. Screening of biodiesel production from waste tuna oil (Thunnus sp.), seaweed Kappaphycus alvarezii and Gracilaria sp.

    Science.gov (United States)

    Alamsjah, Mochammad Amin; Abdillah, Annur Ahadi; Mustikawati, Hutami; Atari, Suci Dwi Purnawa

    2017-09-01

    Biodiesel has several advantages over solar. Compared to solar, biodiesel has more eco-friendly characteristic and produces lower greenhouse gas emissions. Biodiesel that is made from animal fats can be produced from fish oil, while other alternative sources from vegetable oils are seaweed Kappaphycus alvarezii and Gracilaria sp. Waste tuna oil (Thunnus sp.) in Indonesia is commonly a side product of tuna canning industries known as tuna precook oil; on the other hand, seaweed Gracilaria sp. and Kappaphycus alvarezii are commonly found in Indonesia's seas. Seaweed waste that was used in the present study was 100 kg and in wet condition, and the waste oil was 10 liter. The seaweed was extracted with soxhletation method that used n-hexane as the solvent. To produce biodiesel, trans esterification was performed on the seaweed oil that was obtained from the soxhletation process and waste tuna oil. Biodiesel manufactured from seaweed K. alvarezii obtained the best score in flash point, freezing point, and viscosity test. However, according to level of manufacturing efficiency, biodiesel from waste tuna oil is more efficient and relatively easier compared to biodiesel from waste K. alvarezii and Gracilaria sp.

  4. Potential topical natural repellent against Ae. aegypti, Culex sp. and Anopheles sp. mosquitoes

    Directory of Open Access Journals (Sweden)

    Dewi Nur Hodijah

    2014-08-01

    Full Text Available AbstrakLatar belakang:Minyak atsiri daun sirih diketahui mempunyai daya proteksi. Dibuatkan losion berdasarkan pengantar sediaan farmasi yang ditambahkan minyak atsiri daun nilam. Sediaan losion dipilih agar dapat menempel lebih lama di permukaan kulit. Tujuan penelitian ini untuk membandingkan daya proteksi antara losion dengan penambahan minyak nilam dan losion tanpa penambahan minyak nilam dibandingkan daya proteksi dengan DEET. Metode: Penelitian ini merupakan penelitian eksperimental laboratorium. Semua nyamuk uji berasal dari insektarium laboratorium penelitian kesehatan Loka litbang P2B2 Ciamis. Konsentrasi minyak atsiri daun sirih dalam losion adalah 4%; konsentrasi minyak nilam sebagai zat pengikat adalah 0,4%. Formula yang digunakan yaitu formula dasar yang ada pada pengantar sediaan farmasi. Uji repelensi dilakukan dengan menggunakan metoda yang direkomendasikan oleh Komisi pestisida.Hasil: Dihasilkan formulasi losion yang stabil dan masih memenuhi standar formulasi sediaan. Berdasarkan hasil, diperoleh data bahwa DEET dan losion hasil modifikasi memiliki rata-rata daya proteksi di atas 90% selama 6 jam terhadap nyamuk Ae.aegypti dan Culex sp. Kesimpulan: Penambahan minyak nilam pada losion sirih dapat meningkatkan daya proteksi terhadap hinggapan nyamuk Ae. aegypti dan Culex sp. (Health Science Indones 2014;1:44-8Kata kunci:repelen alamiah, minyak atsiri, daun sirih, daun nilam, Ae. aegypti, Culex sp.AbstractBackground: Betel leaf essential oil lotion has been known to have insect repellent properties. A lotion was made based on a pharmaceutical formula from a monograph where patchouli leaf essential oil was added. A lotion preparation was intended to enhance adherence of the formula on the surface of the skin. The purpose of this study was to compare protection percentage of lotion with patchouli oil and without patchouli oil lotion compared to DEET.Methods: This study is an experimental laboratory-based research. All mosquitoes

  5. Caracterización de tilapia roja (Oreochromis sp. con marcadores moleculares RAPD

    Directory of Open Access Journals (Sweden)

    Julieta Torres Jaramillo

    2010-04-01

    Full Text Available Se utilizó la técnica RAPD (amplificación al azar de ADN polimórfico para el estudio de la diversidad genética de Oreochromis sp. (tilapia roja en cinco piscícolas del Valle del Cauca (Colombia y en la determinación del nivel de introgresión de las especies parentales Oreochromis mosambicus, O. niloticus y O. aureus. Se evaluaron 25 cebadores, ocho fueron polimórficos y se obtuvieron 109 bandas. Los valores de heterocigosidad esperada (0.196 a 0.256 y la estructura genética (Gst = 0.22 para Oreochromis sp. indicaron un elevado grado de polimorfismo y alta estructuración genética. Estos resultados fueron consistente con el Fst = 0.268 (P < 0.0001 dado por el Amova y el Gst = 0.040 del análisis de correspondencia múltiple. Los valores de similitud genética, el análisis de grupo, el análisis de correspondencia múltiple y el nivel de introgresion, indicaron diferencias significativas (P<0.0001 en los niveles de introgresión. El bajo nivel de diferenciación genética entre poblaciones podría ser el resultado de peces con el mismo origen genético y la alta variación dentro de poblaciones se puede presentar por prácticas de manejo. La introgresión entre piscícolas es significativa para O. aureus, mientras que las especies O. niloticus y O. mosambicus se encuentran introgresadas de forma similar en las poblaciones de Oreochromis sp.

  6. Transcriptional Regulation of Frizzled-1 in Human Osteoblasts by Sp1.

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    Shibing Yu

    Full Text Available The wingless pathway has a powerful influence on bone metabolism and is a therapeutic target in skeletal disorders. Wingless signaling is mediated in part through the Frizzled (FZD receptor family. FZD transcriptional regulation is poorly understood. Herein we tested the hypothesis that Sp1 plays an important role in the transcriptional regulation of FZD1 expression in osteoblasts and osteoblast mineralization. To test this hypothesis, we conducted FZD1 promoter assays in Saos2 cells with and without Sp1 overexpression. We found that Sp1 significantly up-regulates FZD1 promoter activity in Saos2 cells. Chromatin immunoprecipitation (ChIP and electrophoretic mobility shift (EMSA assays identified a novel and functional Sp1 binding site at -44 to -40 from the translation start site in the FZD1 promoter. The Sp1-dependent activation of the FZD1 promoter was abolished by mithramycin A (MMA, an antibiotic affecting both Sp1 binding and Sp1 protein levels in Saos2 cells. Similarly, down-regulation of Sp1 in hFOB cells resulted in less FZD1 expression and lower alkaline phosphatase activity. Moreover, over-expression of Sp1 increased FZD1 expression and Saos2 cell mineralization while MMA decreased Sp1 and FZD1 expression and Saos2 cell mineralization. Knockdown of FZD1 prior to Sp1 overexpression partially abolished Sp1 stimulation of osteoblast differentiation markers. Taken together, our results suggest that Sp1 plays a role in human osteoblast differentiation and mineralization, which is at least partially mediated by Sp1-dependent transactivation of FZD1.

  7. Occurrence of Pyemotes sp. on Tuta absoluta (Meyrick

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    Carlos Romero Ferreira de Oliveira

    2007-11-01

    Full Text Available The aim of this work was to study the population reduction of the tomato leafminer moth, Tuta absoluta (Meyrick, by Pyemotes sp. in the laboratory. The mite became greenish when fed on caterpillars, pupae, and adults of T. absoluta. The caterpillars and adults of T. absoluta were quickly paralyzed by the mite venom. A single T. absoluta could host many Pyemotes sp, physogastric females, which were allowed to grow on the moth. Pyemotes sp. can be a new alternative for the biological control of T. absoluta. However, this possibility must be better understood before it could be recommended, because Pyemotes sp. could also cause dermatitis in the humans.O ácaro Pyemotes sp. (Acari: Pyemotidae é um parasita de várias espécies de insetos e exibe o processo de fisogastria na reprodução. É registrada a ocorrência de Pyemotes sp. sobre a traça-do-tomateiro, Tuta absoluta (Meyrick, que sofreu redução considerável de suas populações em condições de laboratório. Este ácaro foi observado parasitando larvas, pupas e adultos de T. absoluta e suas fêmeas fisogástricas apresentaram coloração esverdeada. As larvas e adultos de T. absoluta atacados por Pyemotes sp. exibiram paralisia em poucos minutos, devido à injeção de toxinas por parte do ácaro. Observou-se que um único indivíduo de T. absoluta pode suportar muitas fêmeas fisogástricas de Pyemotes sp., permitindo que outra geração do ácaro continuasse a se reproduzir. O ácaro Pyemotes sp. surge como uma possibilidade para o controle biológico de T. absoluta, mas sua utilização deve ser melhor avaliada já que é uma espécie causadora de dermatites em humanos.

  8. Prevention of White Spot Syndrome Virus Infection on Penaeus monodon by Immersion in CEPM Extract of Avicennia sp. and Sonneratia sp.

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    D. Wahjuningrum

    2007-01-01

    Full Text Available The quality and survival rate are still being the problem that hampers the productivity of black tiger shrimp, Penaeus monodon culture.  Impaired quality of larval shrimp and environmental conditions can confer shrimp be infected by diseases, including viruses such as white spot syndrome virus (WSSV.  Prevention of disease infection using chemicals can offer negative impacts on water, pathogen resistance and consumers.  This study was conducted to examine the efficacy of an alternative prevention compound as liquid mangrove extract (CEPM from Avicennia sp. and Sonneratia sp. By immersion in different dose of CEPM, i.e. 250, 500, 750 and 1000 ppm, the patogenicity of WSSV was found to be different.  Patogenicity of WSSV decreased after treatment by CEPM, hence this could be used to induce shrimp immunity.  Optimum dose of CEPM was 250 ppm, which could increased survival rate of shrimp after challenging by WSSV, up to 98.4% shrimp survived. Keywrods: WSSV, black tiger shrimp, extract, Avicennia sp., Sonneratia sp.   ABSTRAK Kualitas dan kelangsungan hidup merupakan masalah yang masih membatasi produktivitas budidaya udang windu Penaeus monodon. Kondisi udang dan kualitas lingkungan yang kurang baik dapat menyebabkan udang terserang penyakit, termasuk yang disebabkan oleh virus termasuk white spot syndrome virus (WSSV. Upaya pengendaliannya menggunakan bahan kimia secara berlebih dapat menimbulkan dampak negatif bagi lingkungan perairan, kesehatan konsumen dan menimbulkan resistensi patogen. Penelitian ini dilakukan untuk mengetahui efektivitas bahan alternatif berupa cairan ekstrak pohon mangrove (CEPM dari jenis Avicennia sp. dan Sonneratia sp. sebagai upaya pencegahan. Dengan perendaman beberapa konsentrasi yang berbeda (250, 500, 750 dan 1000 ppm penggunaan cairan ekstrak pohon mangrove (CEPM Avicennia sp. dan Sonneratia sp., memberikan pengaruh yang berbeda terhadap patogenitas WSSV dan udang uji pada setiap perlakuan. Tingkat

  9. DAN IDENTIFIKASI PATOGEN POTENSIAL YANG MENGINFEKSI IKAN RAINBOW (Melanotaenia sp.

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    Lili Sholichah

    2014-03-01

    Full Text Available Pemeliharaan ikan rainbow (Melanotaenia sp. di Balai Penelitian dan Pengembangan Budidaya Ikan Hias selalu terjadi kematian secara bertahap mulai calon induk hingga proses pemijahan. Hal ini terjadi berulang kali sehingga ketersediaan induk Melanotaenia sp. sangat terancam. Ikan ini berasal dari Papua yang diperoleh mengandalkan penangkapan di alam. Tujuan dari penelitian ini adalah untuk menginventarisir dan mengidentifikasi berbagai patogen (parasit, jamur, bakteri potensial yang menginfeksi ikan rainbow yang dipelihara di dalam akuarium berukuran 50 cm x 50 cm x 50 cm dengan sistem aliran air stagnan. Tiga jenis rainbow yang dipelihara yaitu: rainbow Sungai Salawati, asal Sungai Sawiat, dan asal Danau Kurumoi. Setiap ikan masing-masing berjumlah 100 ekor dipelihara di akuarium dengan penambahan batu karang dan tanpa penambahan karang (kontrol ke dalam akuarium. Ikan diberi pakan sekenyangnya berupa jentik nyamuk dan cacing rambut beku setiap pagi dan sore hari. Sampling dilakukan secara random sebulan sekali dan secara unrandom setiap ada kejadian ikan sakit. Gejala klinis ikan yang sakit sebagai berikut: ikan berenang di permukaan dan menggosok-gosokkan badan di dinding akuarium, nafsu makan berkurang, gerakan berputar-putar, warna memudar menjadi putih, penekanan warna hitam pada sirip punggung dan perut meningkat, pendarahan pada perut, lendir berlebihan dan sangat berbau, serta sisik berdiri/terbuka. Diagnosa dan deteksi penyakit awal berupa pengamatan parasit baik ektoparasit maupun endoparasit, pengamatan dan isolasi jamur pada media selektif jamur, dan isolasi bakteri dilakukan untuk mengetahui jenis-jenis patogen yang menginfeksi ketiga jenis ikan rainbow. Selanjutnya dilakukan uji histologi dan analisa DNA beberapa patogen. Hasil pengamatan diperoleh patogen berupa parasit (Ichthyophthirius sp., Dactylogyrus sp., Gyrodactylus sp., dan Trichodina sp. dan bakteri (Aeromonas hydrophila, Acinetobacter sp

  10. Impacts of varying light regimes on phycobiliproteins of Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 isolated from diverse habitats.

    Science.gov (United States)

    Kannaujiya, Vinod K; Sinha, Rajeshwar P

    2015-11-01

    The adaptability of cyanobacteria in diverse habitats is an important factor to withstand harsh conditions. In the present investigation, the impacts of photosynthetically active radiation (PAR; 400-700 nm), ultraviolet-B (UV-B; 280-315 nm), and PAR + UV-B radiations on two cyanobacteria viz., Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 inhabiting diverse habitats such as hot springs and rice fields, respectively, were studied. Cell viability was about 14 % in Nostoc sp. HKAR-2 and Nostoc sp. HKAR-11 after 48 h of UV-B exposure. PAR had negligible negative impact on the survival of both cyanobacteria. The continuous exposure of UV-B and PAR + UV-B showed rapid uncoupling, bleaching, fragmentation, and degradation in both phycocyanin (C-PC) and phycoerythrin (C-PE) subunits of phycobiliproteins (PBPs). Remarkable bleaching effect of C-PE and C-PC was not only observed with UV-B or PAR + UV-B radiation, but longer period (24-48 h) of exposure with PAR alone also showed noticeable negative impact. The C-PE and C-PC subunits of the rice field isolate Nostoc sp. HKAR-11 were severely damaged in comparison to the hot spring isolate Nostoc sp. HKAR-2 with rapid wavelength shifting toward shorter wavelengths denoting the bleaching of both the accessory light harvesting pigments. The results indicate that PBPs of the hot spring isolate Nostoc sp. HKAR-2 were more stable under various light regimes in comparison to the rice field isolate Nostoc sp. HKAR-11 that could serve as a good source of valuable pigments to be used in various biomedical and biotechnological applications.

  11. Impact of serum SP-A and SP-D levels on comparison and prognosis of idiopathic pulmonary fibrosis: A systematic review and meta-analysis.

    Science.gov (United States)

    Wang, Kai; Ju, Qing; Cao, Jing; Tang, Wenze; Zhang, Jian

    2017-06-01

    Idiopathic pulmonary fibrosis (IPF) has a poor prognosis in general; however, it is heterogeneous to detect relative biomarkers for predicting the disease progression. Serum biomarkers can be conveniently collected to detect and help to differentially diagnose IPF and predict IPF prognosis. This meta-analysis aimed to evaluate the use of serum surfactant proteins A and D (SP-A and SP-D) for differential diagnosis and prognosis of IPF. Relevant articles were searched in PubMed, Embase, and Chinese National Knowledge Infrastructure databases and reviewed by 2 independent readers. Standard mean difference (SMD) and 95% confidence interval (CI) were calculated to assess the difference in serum levels of SP-A/D among patients with IPF, when compared to patients with non-IPF interstitial lung disease (ILD), pulmonary infection, and healthy control. Hazard ratio (HR) and 95% CI were used to compare the relative risk of mortality. Twenty-one articles (totalling 1289 IPF patients) were included in final meta-analysis. Serum SP-A levels were significantly higher in patients with IPF than in patients with non-IPF ILD (SMD: 1.108 [0.584, 1.632], P infection (SMD: 1.320 [0.999, 1.640], P SMD: 2.802 [1.901, 3.702], P SMD: 0.459 [-0.000, 0.919], P = .050). Serum SP-D levels were significantly higher in patients with IPF than in patients with pulmonary infection (SMD: 1.308 [0.813, 1.803], P SMD: 2.235 [1.739, 2.731], P < .001). Risk of death in patients with IPF and elevated serum SP-A was increased 39% compared to patients with low SP-A groups. Elevated SP-D increased risk by 111% when compared to low SP-D. In acute exacerbation of IPF, serum SP-A/D were higher than those in stable stage. The comparisons and prognosis might be different in Asian and Caucasian patients. Serum SP-A/D detection might be useful for differential diagnosis and prediction of survival in patients with IPF.

  12. Genetic polymorphism of horse serum protein 3 (SP3).

    Science.gov (United States)

    Juneja, R K; Sandberg, K; Kuryl, J; Gahne, B

    1989-01-01

    Two-dimensional agarose gel (pH 8.6)-horizontal polyacrylamide gel (pH 9.0) electrophoresis of horse serum samples, followed by general protein staining, revealed genetic polymorphism of an unidentified protein tentatively designated serum protein 3 (SP3). The SP3 fractions appeared distinctly when a 14% concentration of acrylamide was used in the separation gels. The 2-D mobilities of SP3 fractions were quite similar to that of albumin. Family data were consistent with the hypothesis that the observed SP3 phenotypes were controlled by four co-dominant, autosomal alleles (D, F, I, S). Evidence was provided that the F allele can be further divided into two alleles (F1 and F2); the mobilities of F1 and F2 variants were very similar. Each of the SP3 alleles gave rise to one fraction and each of the heterozygous types showed two fractions. More than 600 horses representing five different breeds (Swedish Trotter, North-Swedish Trotter, Thoroughbred, Arab and Polish Tarpan) were typed for SP3, and allele frequency estimates were calculated. SP3 was highly polymorphic in all breeds studied.

  13. PENGGUNAAN PROTEASE ASPERGILLUS sp. DAN RHIZOPUS sp. DENGAN KONSENTRASI YANG BERBEDA DALAM TAHAPAN UNHAIRING TERHADAP KUALITAS FISIK DAN LIMBAH CAIR PADA PENYAMAKAN KULIT DOMBA

    Directory of Open Access Journals (Sweden)

    Yunus Syafie

    2013-10-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui aktivitas proteolitik yang dihasilkan jamur Aspergillus sp. dan Rhizopus sp. dalam tahapan unhairing (buang rambut pada proses penyamakan kulit domba serta pengaruh penggunaan dengan konsentrasi berbeda, terhadap kuat tarik, kemuluran, suhu kerut, dan kualitas limbah (pH, BOD, dan COD. Materi yang digunakan yaitu 15 lembar kulit domba awetan garam dibagi 2 bagian sepanjang garis lurus punggung sehingga diperoleh 30 lembar kulit, kulit dibagi secara acak menjadi 10 kelompok. Perlakuan terdiri dari dua belas kombinasi yaitu protease dari Aspergillus sp., Rhizopus sp. serta gabungan antara Aspergillus sp. dan Rhizopus sp. dengan konsentrasi protease 2% (P1, 2,5% (P2, 3% (P3, dan sebagai kontrol P0. Proses unhairing secara konvensional menggunakan bahan kimia Na2S (3% dan kapur Ca(OH2 6% dengan 3 ulangan. Sampel air limbah setelah proses unhairing diambil dan dibawa ke laboratorium untuk uji kualitas. Kulit diproses lebih lanjut menjadi kulit samak glazed. Data yang diperoleh dianalisis menggunakan Rancangan Acak Lengkap pola faktorial 3 x 4, apabila berbeda nyata dilakukan uji banding dengan uji Duncan’s new Multiple Range Test (DMRT. Hasil uji aktivitas proteolitik paling tinggi adalah gabungan antara protease dari Aspergillus sp. dan Rhizopus sp. yaitu sebesar 1.079,17 μM/ml/menit, sedangkan protease Aspergillus sp. dan Rhizopus sp. masing-masing memiliki aktivitas proteolitik sebesar 542,96 μM/ml/menit dan 392,89 μM/ml/menit. Hasil penelitian menunjukkan bahwa penggunaan protease dengan konsentrasi yang berbeda dapat memberikan efek yang positif terhadap kualitas fisik dan limbah cair proses unhairing kulit domba. Konsentrasi protease 2,5% dan 3% dapat meningkatkan nilai kuat tarik dan suhu kerut kulit domba serta menghasilkan kulit yang bersih tanpa ada rambut yang menempel dan struktur serabut kolagen terbuka. Perlakuan protease sangat potensial karena dapat menekan angka BOD dan COD limbah

  14. Possible linkage of SP6 transcriptional activity with amelogenesis by protein stabilization.

    Science.gov (United States)

    Utami, Trianna W; Miyoshi, Keiko; Hagita, Hiroko; Yanuaryska, Ryna Dwi; Horiguchi, Taigo; Noma, Takafumi

    2011-01-01

    Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis) are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis. However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis.

  15. Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization

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    Trianna W. Utami

    2011-01-01

    Full Text Available Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis. However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis.

  16. The nature of ethnic identity among the people of Curaçao

    NARCIS (Netherlands)

    Dijs, N.M. van der

    2011-01-01

    This qualitative research explores the nature of ethnic identity among the people of Curaçao. The questions that are of fundamental interest to this study are: how do the people of Curaçao perceive themselves ethnically, how do they experience their sense of belonging to an ethnic group and what

  17. Chaenorhinum semispeluncarum sp. nov. and C. yildirimlii sp. nov. (Scrophulariaceae) from east Anatolia, Turkey

    DEFF Research Database (Denmark)

    Yildirim, Hasan; Tan, Kit; Senol, Serdar

    2010-01-01

    Chaenorhinum semispeluncarum H. Yildirim, Kit Tan, S. Senol & A. Pirhan sp. nov. and C. yildirimlii Kit Tan, H. Yildirim, S. Senol & A. Pirhan sp. nov. (Scrophulariaceae, C. sect. Microrrhinum) from east Anatolia are described and illustrated. They are both narrow endemics related to the rare C. ...

  18. Production of microbial biomass protein by sequential culture fermentation of Arachniotus sp., and Candida utilis

    International Nuclear Information System (INIS)

    Ahmed, S.; Ahmad, F.; Hashmi, A.S.

    2010-01-01

    Sequential culture fermentation by Arachniotus sp. at 35 deg. C for 72 h and followed by Candida utilis fermentation at 35 deg. C for 72 h more resulted in higher production of microbial biomass protein. 6% (w/v) corn stover, 0.0075% CaCl/sub 2/.2H/sub 2/O, 0.005% MgSO/sub 4/.7H/sub 2/O, 0.01% KH/sub 2/PO/sub 4/, C:N ratio of 30:1 and 1% molasses gave higher microbial biomass protein production by the sequential culture fermentation of Arachniotus sp., and C. utilis. The mixed microbial biomass protein produced in the 75-L fermentor contained 16.41%, 23.51%, 10.9%, 12.11% and 0.12% true protein, crude protein, crude fiber, ash and RNA content, respectively. The amino acid profile of final mixed microbial biomass protein showed that it was enriched with essential amino acids. Thus, the potential utilization of corn stover can minimize the cost for growth of these microorganisms and enhance microbial biomass protein production by sequential culture fermentation. (author)

  19. Aplicabilidade de geotecnologias para subsidiar a gestão dos recursos hídricos em Americana (SP / Applicability of geo-technologies to subsidize hydric resources management in the city of Americana (SP

    Directory of Open Access Journals (Sweden)

    Debora Cristina Cantador

    2017-05-01

    Full Text Available Ao analisar as águas urbanas tendo como recorte territorial o município, vários aspectos precisam ser levados em consideração. Assim pode-se entender como se realiza a gestão e, principalmente, como se encontra a qualidade, a preservação e a conservação dos cursos d’água. Esses aspectos devem estar relacionados, principalmente, com as legislações existentes que servem de subsídios para os gestores. Além disso, entende-se que a dinâmica espacial também auxilia na compreensão e visualização de como os recursos hídricos e os elementos associados a eles se comportam de forma mais ampla. Neste trabalho, buscou-se abordar sobre que modo as geotecnologias podem contribuir como um instrumento importante para a análise da atual situação da gestão dos recursos hídricos, usando como área de estudo o município de Americana (SP.

  20. Herdabilidades para ganho de peso da desmama ao sobreano e perímetro escrotal ao sobreano e tendências genética e fenotípica para ganho de peso da desmama ao sobreano em bovinos Nelore-Angus Heritabilities for post-weaning daily gain and scrotal circumference at yearling age and genetic and phenotypic trends for post-weaning daily gain in Nellore-Angus crossbreds

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    Arione Augusti Boligon

    2006-08-01

    Full Text Available Com o objetivo de estimar herdabilidades para ganho de peso médio diário da desmama ao sobreano (GMDDS e para perímetro escrotal ao sobreano (PES e tendências genética e fenotípica para GMDDS, foram utilizados 47.668 registros de peso e de ganho de peso de uma população multirracial Nelore-Angus, coletados entre 1991 e 2001 em diversas regiões do Brasil. Os dados foram analisados pelo método REML e as estimativas das (covariâncias foram obtidas por meio de um modelo animal, no qual foram considerados fixos os efeitos da composição racial do animal (obtida pela concatenação do percentual da raça Nelore do próprio animal, de seu pai e de sua mãe e do grupo de contemporâneos pós-desmama (animais nascidos no mesmo rebanho, ano, época e pertencentes ao mesmo sexo e grupo de manejo e, como aleatórios, os efeitos genético aditivo direto e residual. A herdabilidade para PES foi estimada utlizando-se o mesmo modelo, acrescido dos efeitos fixos do peso e da idade do animal ao sobreano (covariáveis. As médias para idade nas pesagens foram 215 e 528 dias para a desmama e o sobreano, respectivamente. A herdabilidade estimada para GMDDS foi 0,44 ± 0,02 e para PES, 0,22 ± 0,08. A tendência genética anual predita para GMDDS foi decrescente até 1996 e crescente a partir desse período. A tendência fenotípica anual foi de 9,4 g/dia/ano.Data consisting of 47.668 records of a Nellore-Angus crossbred population, raised in several regions of Brazil, from 1991 to 2001, were used to estimate heritability for post-weaning daily gain (ADG and for scrotal circumference at yearling age (SCY using REML. Genetic and phenotypic trends for ADG were also estimated. The model used to estimate heritability and breeding values (BVs for ADG included the fixed effects of breed composition (defined by the percentage of contribution of the Nellore breed of the animal and his parents and the contemporary group after weaning (herd, year/season of birth