Tanaka, T; Yamanaka, S; Takinami, K
For saccharification of cellulose, an acetolysis method using assimilable acid with a microorganism was applied. Based on this method, a new method which gave totally assimilable products was established. The rigid crystalline structure of cellulose was disrupted by acetolysis with 2-2.5 times as much acetic anhydride as cellulose on a weight basis and 1 N sulfuric acid as a catalyst. Then for cleavage of O-acetyl ester and glycosidic bonds, the resulting amorphous acetolysate of cellulose could easily be hydrolyzed by heating in 1 N sulfuric acid at 120/sup 0/C for 1-1.5 h without over-disruption of glucose. Ninety-eight % of the cellulose used was recovered in the form of hydrolysate having about 30% saccharide concentration. The hydrolysate obtained was composed of 74% glucose, 13% cellobiose and 11% mono-O-acetyl glucose on a weight basis.
Asperger, S. (Research Center of the Croatian Academy of Sciences and Arts, Zagreb (Croatia)); Kukric, Z.; Sutic, D. (Sarajevo Univ. (Yugoslavia). Faculty of Natural Sciences and Mathematics); Saunders, W.H. Jr. (Rochester Univ., NY (United States). Dept. of Chemistry)
Acetolysis and formolysis of dideuterioferrocenylmethyl benzoate exhibit large secondary deuterium kinetic isotope effects and an abnormal temperature dependence. In the presence of LiClO[sub 4], which prevents the reversion from solvent-separated to contact ion-pairs, K[sub H]/K[sub D] at 25 [sup o]C amount to 1.53 [+-] 0.02 (acetolysis) and 1.48 [+-] 0.03 (formolysis). In the presence of LiClO[sub 4] the ratios of Arrhenius pre-exponential factors, A[sub H]/A[sub D], are significantly less than unity and amount to 0.49 [+-] 0.01 (acetolysis) and 0.38 [+-] 0.04 (formolysis). In the absence of LiClO[sub 4] the A[sub H]/A[sub D] ratios are much smaller (0.02 both in acetolysis and formolysis). We suggest that these surprisingly low values result from a change in rate-determining step over the temperature range, from formation of the solvent-separated ion-pair at low temperatures to reaction of the dissociated carbocation with solvent at the highest temperatures. Whether tunnelling plays any role in these solvolyses is discussed. (Author).
Kobayashi, H; Shibata, N; Mitobe, H; Ohkubo, Y; Suzuki, S
Structural analysis of the phosphomannan isolated from yeast-form cells of a pathogenic yeast, Candida albicans J-1012 strain, was conducted. Treatment of this phosphomannan (Fr. J) with 10 mM HCl at 100 degrees C for 60 min gave a mixture of beta-1,2-linked manno-oligosaccharides, from tetraose to biose plus mannose, and an acid-stable mannan moiety (Fr. J-a), which was then acetolyzed by means of an acetolysis medium, 100:100:1 (v/v) mixture of (CH3CO)2O, CH3COOH, and H2SO4, at 40 degrees C for 36 h in order to avoid cleavage of the beta-1,2 linkage. The resultant manno-oligosaccharide mixture was fractionated on a column of Bio-Gel P-2 to yield insufficiently resolved manno-oligosaccharide fractions higher than pentaose and lower manno-oligosaccharides ranging from tetraose to biose plus mannose. The higher manno-oligosaccharide fraction was then digested with the Arthrobacter GJM-1 alpha-mannosidase in order to cleave the enzyme-susceptible alpha-1,2 and alpha-1,3 linkages, leaving manno-oligosaccharides containing the beta-1,2 linkage at their nonreducing terminal sites, Manp beta 1----2Manp alpha 1----2Manp alpha 1----2Manp alpha 1----2Man, Manp beta 1----2Manp beta 1----2Manp alpha 1----2Manp alpha 1---- 2Manp alpha 1----2Man, and Manp beta 1----2Manp beta 1----2Manp beta 1----2Manp alpha 1---- 2Manp alpha 1----2Manp alpha 1----2Man. However, the result of acetolysis of Fr. J-a by means of a 10:10:1 (v/v) mixture of (CH3CO)2O, CH3COOH, and H2SO4 at 40 degrees C for 13 h was significantly different from that obtained by the mild acetolysis method; i.e., the amount of mannose was apparently larger than that formed by the mild acetolysis method. In summary, a chemical structure for Fr. J as a highly branched mannan containing 14 different branching moieties was proposed.
Cell wall mannans were isolated from 8 different Candida species and separated in oligosaccharides by partial acetolysis. After gel chromatography specific acetolysis patterns were obtained. The 13 C NMR spectra of mannans and oligosaccharides were recorded. Signals at delta = 93.1 - 105.4 were assigned to certain chemical structures. Both the spectral patterns and the acetolysis patterns of the yeast mannans can be used for the discrimination of related yeasts. (author)
1. A simple technique for acetolysis of small quantities of polliniferous (herbarium) material is described and notes on pollen photomicrography are presented. 2. Pollen grains of Sarawakodendron and six related genera, consisting of twenty-nine mostly Malesian species, have been examined and
Eda, S; Kato, K
The structure of an arabinoxyloglucan, separated from the hemicellulosic polysaccharides of the midrib of the leaves of Nicotiana tabacum, was investigated by methylation analyses before and after mild acid hydrolysis, acetolysis and cellulase-degradation. The arabinoxyloglucan consists of L-arabinose, D-xylose and D-glucose in a molar ratio of 13:33:54, and has a backbone of ..beta..-(1..-->..4)-linked D-glucopyranosyl residues. Some of the glucopyranosyl residues are attached at the 6 position by single ..cap alpha..-D-xylopyranosyl and ..cap alpha..-L-arabinofuranosyl-(1..-->..2)-..cap alpha..-D-xylopyranosyl side chains.
Ebráilon Masetto; Maria Luisa Lorscheitter
This paper presents the results of a qualitative palynological analysis of a 140 cm-thick section of Holocene sediments from a paleolagoon, representing the last 2600 years, taken from an outcrop at Hermenegildo Beach (33º42'S; 53º18'W), located in the municipality of Santa Vitória do Palmar, in the state of Rio Grande do Sul, Brazil. Samples were treated with hydrochloric acid, hydrofluoric acid and potassium hydroxide, after which they were subjected to acetolysis and mounted on glycerin-co...
Marcos Gonçalves Ferreira
Full Text Available We applied an "adapted" protocol for collecting and processing pollen grains in the pollen analysis of the post-emergence residue of Melipona (Melikerria interrupta Latreille. The study was conducted at the Sant'Ana honey farm, located on the banks of the Solimões River, in the municipality of Manacapuru, in the state of Amazonas, Brazil, where a colony was monitored in October and November 2010. From that colony, 10 samples of post-emergence residue were collected. Unlike in the acetolysis method, there was no need to expose pollen grains to an acidic medium, because pollen loses its content during the larval digestive process. We identified 32 pollen types, from 19 botanical families, plus three undetermined pollen types. The most representative family was Fabaceae (Mimosoideae, with eight pollen types, Mimosa guilandinae being the most common species. Only the pollen of Miconia (Melastomataceae, with 74.10%, was classified as a common pollen. We also found that the pollen of Mimosa pudica (Fabaceae: Mimosoideae retained its content, indicating that not all resources furnished by workers are utilized by the larvae. The protocol applied here, despite omitting the acetolysis process, was efficient, providing full details of pollen contained in post-emergence residue.
Full Text Available Pollen morphology of 20 species belonging to Onobrychis sect. Heliobrychis was studied in Iran. The pollen grains from the herbarium specimens were prepared by acetolysis method and then 8 quantitative characters were studied by light microscopy. Data analysis was carried out by cluster analysis and principal component analysis methods. Pollen grains of the sections were 3-colpate with reticulate ornamentation. Results of the study showed that although the pollen grains in the section were comparatively homogenous, but had a considerable variation in the studied quantitative characters. However among the studied characters, equatorial and polar diameters of pollen grains were the most significant characters. The members of the section can be divided into 3 groups based on these characters.
UJI VIABILITAS DAN PERKEMBANGAN SERBUK SARI BUAH NAGA PUTIH (HYLOCEREUS UNDATUS (HAW. BRITTON & ROSE, MERAH (HYLOCEREUS POLYRHIZUS (WEB. BRITTON & ROSE DAN SUPER MERAH (HYLOCEREUS COSTARICENSIS (WEB. BRITTON & ROSE SETELAH PENYIMPANAN
NI KADEK YUNITA SARI
Full Text Available The aim of the research was to determine pollen viability, pollen tube length and pollen development of white, red and super red dragon fruit after storage at different temperatures and times. The method used to test pollen viability was hanging drop technique and to observe the development of pollen used acetolysis techniques. The results showed viability and pollen tube length of white, red and super red dragon fruit after storage at temperature of 10° C and -20° C for 1, 2 and 3 weeks decreased (66% and 25%, tended to increase (2% after 4 weeks. Viability and pollen tube length decreased (100% after storage at 30° C for 4 weeks. Pollen development of white, red and super red dragon fruit after storage at 30°C, 10°C and -20°C for 1 to 4 weeks showed the majority of pollen consists of uninucleat and binucleat.
Full Text Available This paper presents the results of a qualitative palynological analysis of a 140 cm-thick section of Holocene sediments from a paleolagoon, representing the last 2600 years, taken from an outcrop at Hermenegildo Beach (33º42'S; 53º18'W, located in the municipality of Santa Vitória do Palmar, in the state of Rio Grande do Sul, Brazil. Samples were treated with hydrochloric acid, hydrofluoric acid and potassium hydroxide, after which they were subjected to acetolysis and mounted on glycerin-coated slides for light microscopy analysis. Among the 48 palynomorphs identified were 25 fungi, eight algae, three bryophytes, and 12 pteridophytes. Brief descriptions and illustrations of each palynomorph are presented, together with ecological data from the organism of origin when possible. Our findings will serve as reference material for paleoenvironmental studies in the coastal plain of southern Brazil.
André Rodrigo Rech
Full Text Available Bees and angiosperms established a mutualistic relationship along the evolutionary time. The aim of this study is to contribute for the understanding of this relation analyzing pollen stored by stingless bees colonies distributed along the Rio Negro. Fourteen species of Meliponini from the genera Partamona, Scaura, and Trigona were studied with regard to the content of pollen pots. The pollen material was removed from the pollen pots, homogenized, and prepared according to the usual acetolysis technique. The overlap of the trophic niche and the grouping of species by similarity of niches was calculated. The identification revealed 78 pollen types belonging to 36 families, being 37 types attractive and 16 considered as promoters of a temporary specialization event. With the results, it was possible to indicate a list of important plants for meliponiculture in the Amazon.
Mohd-Arrabe' , A. B.; Noraini, Talip Noraini [School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia)
Rhizophora L. are common mangrove genus in Peninsular Malaysia, it contains 3 species and 1 hybrid (R. apiculata Blume, R. mucronata Lam., R. stylosa Griff., R. x lamarckii Montrouz). This genus has some unique adaptation towards extreme environment. Rhizophora has looping aerial stilt-root and uniformly viviparous. The aim of this study is to investigate the variation in the pollen morphology of Rhizophora that can be related to their habitat. Methods include in this study is pollen observation under light and acetolysis method under scanning electron microscope. Pollen type of Rhizophora species studied except hybrid species is classified tricolporate, shape spheroidal based on ratio of length polar axis/ length of equatorial axis (1.03 - 1.09). The exine ornamentation is perforate-reticulate for R. apiculata and R. mucronata, while R. stylosa is perforate. For the only hybrid in Peninsular Malaysia, R. x lamarckii (R. apiculata x R. stylosa) differs from others, tricolpate with the absence of porate, shape is subprolate and exine ornamentation is reticulate and striate in equatorial region. Pollenkitt is present due to the salty and extreme environment. This may enhance the volume of pollenkitt present surrounding the pollen grains in Rhizophora for protection and adaptation purposes. Based on these findings, it is evident that pollen morphology is somehow related to its natural habitat.
CARACTERIZACIÓN CUALITATIVA DEL POLEN RECOLECTADO POR Apis mellifera L EN TRES APIARIOS DEL MUNICIPIO DE POPAYÁN QUALITATIVE CHARACTERIZATION OF THE GATHERED POLLEN FOR Apis mellifera L IN THREE APIARIES IN THE MUNICIPALITY OF POPAYAN
NELSON JOSÉ VIVAS
Full Text Available Durante los meses de Junio, Julio, Agosto y Septiembre de año 2006, se realizó un estudio de melisopalinología, en tres veredas del municipio de Popayán, con el fin de determinar la flora apícola predominante en la zona, se analizaron los tipos polínicos recolectados por las abejas Apis mellifera y los botones florales recolectados en campo durante la época de estudio mediante el método de Acetolisis láctica; de cuarenta tipos polínicos recolectados, mediante microscopía se identificaron 24 en el polen de las colmenasDuring months of June, July, August September in 2006, a study of melisopalinology was developed in three places near Popayán, aiming to determine the predominant apian flora in the study zone. The pollen types recollected by Apis mellifera and the flower blooms collected on the field during the study were analyzed using the Lactic Acetolysis technique. From the 40 types of pollen collected, by means of microscopy only 24 were identified among the pollen found in the beehives
Full Text Available Sensitivity of three Scenedesmus strains exposed to aqueous fuel-oil extract (AFOE is strongly strain-dependent S. quadricauda is the most resistant, S. armatus moderately tolerant whereas the most sensitive appears to be S. microspina. The sensitivity of tested species increases parallel with decreasing of cell size and cell number in coenobium. The values of the cell surface/cell volumes ratios only partly explain the above relationships. Electron microscope investigations reveal that the sensitivity may depend on cell wall structure of the strains. Cell wall of all here investigated strains is built of two layers: the inner so-called cellulosic layer and the outer one showing a three-laminar structure (TLS. The latter contains an acetolysis-resistant biopolymer (ARB. These two layers are similar in thickness in the three strains tested, but the surface of Scenedesmus is covered with various epistructures that are characteristic of strains. Some of them as the tightly fitting warty layer of S. armatus and especially the loosely fitting reticulate layer of S. quadricauda may contribute to lower permeability of cell wall. The structure of the rosettes also appears to be correlated with the sensitivity of strains. Presence of invaginations of plasmalemma in areas under rosettes indicates their role in transport processes inside/outside the cells.
Amirul-Aiman, A. J.; Noraini, T.; Nurul-Aini, C. A. C.; Idris, S.; Suhaniza, R.
Firmiana malayana also known as "Bullocks eye or Mata Lembu" in Malaysia and can be found along riverbanks and open forests in Peninsular Malaysia and seldom planted in populated areas. The flowers of the Firmiana malayana are vivid orange in colour, on tassels up to 12 cm long. Usually this species will shed its leaves after a dry period and remains bare for six to eight weeks. The objective of this study is to determine the pollen morphological characteristics of the Firmiana malayana in order to add more information on the species under the family of Sterculiaceae in Malaysia. Methods for this study includes acetolysis technique for the pollens and viewed under light microscope and scanning electron microscope. Results shown that the pollens of the species Firmiana malayana appeared to be monad and dyad with tricolporate class with both porate and colpus present. The shape of this species is prolate with P/E index of 1.49. This species was considered as medium-size pollens as the pollens ranges from 26 - 36 µm. The ornamentation of the pollen is reticulate where the ornamentation is network-like pattern formed by exine elements of lumen and murus. Based on the results obtained, pollen morphology is a great tool that can aid in plant identification and classification as well having taxonomic values.
Dórea, Marcos da C; Dos Santos, Francisco de A R; Lima, Luciene C de L E; Figueroa, Luís E R
A new treatment protocol was developed to analyze pollen residues found in nests of Centris tarsata Smith harvested from nest-traps. The study area was located in the Canudos Biological Station in the municipality of Canudos (09 masculine56'34'S; 38 masculine59'17'W), in the northeastern micro-region of Bahia State, Brazil. The local vegetation is an open caatinga (deciduous dryland vegetation), the regional climate is semi-arid, the average annual temperature is 24.1 masculineC, and the annual regional rainfall rate is 454 mm. Ten nests of C. tarsata were collected in trap-nests during the first semester of 2004. Pollen analysis from the nests required the development of a new methodology that combined techniques of palynological sediment analysis with the more common pollinic analysis by acetolysis. Microscopic analyses employed optical microscopy techniques. The pollinic spectrum of the samples from C. tarsata indicated the presence of 17 pollen types from seven plant families, which were present in assemblage of five to eleven pollen types, pointed to the plants used by bees to feed on their offspring. The most represented plant families were Leguminosae (49.3%) and Solanaceae (43.2%). The most frequent pollen types in the samples were from Solanum paniculatum (43.8%) and Senna rizzini (32.1%). The protocol developed provides a new tool for diet assessment of Centris and other groups of solitary bees.
Sodré, Geni da S; Marchini, Luís C; Carvalho, Carlos A L de; Moreti, Augusta C de C C
Knowledge about the botanical source of honey is very important for the beekeeper while it indicates adequate and abundant supply sources of nectar and pollen for the bees, thus contributing toward improved yield. The present study means to identify the pollen types occurring in 58 samples of honey produced in two states of the northeastern region of Brazil, Piauí (38 samples) and Ceará (20 samples), and to verify the potential of the honey plants during the months of February to August. The samples were obtained directly from beekeepers in each state and analyzed at the Apiculture Laboratory of the Entomology Section of Escola Superior de Agricultura "Luiz de Queiroz", USP, Piracicaba, State of São Paulo, Brazil. The pollen analysis was performed using the acetolysis method. The samples were submitted to both a qualitative and a quantitative analysis. The dominant pollen in the State of Ceará is from Mimosa caesalpiniaefolia, M. verrucosa, Borreria verticillata, Serjania sp., and a Fabaceae pollen type, while in the State of Piauí it is from Piptadenia sp., M. caesalpiniaefolia, M. verrucosa, Croton urucurana and Tibouchina sp.
Larissa Silva Souza
Full Text Available Regional-level studies of floral resources used by social bees for honey production can contribute to the improvement of management strategies for bee pastures and colonies, by identifying the most visited flowers and thus characterizing the various geographical origins of honey. The objective of this study was to investigate, through pollen analysis, the types of pollen and nectar sources used by the uruçu bee (Melipona scutellaris L. in the North Coast of Bahia. Honey samples were taken monthly from five colonies in an apiary from August 2010 to July 2011. Pollen analysis of honey was performed by using the acetolysis method, followed by qualitative and quantitative analysis of pollen grains. Fifty pollen types belonging to 40 genera and 17 families were identified. The results indicate predominance of pollen types belonging to the families Fabaceae and Myrtaceae, which suggests that the bees preferred foraging from trees and shrubs. These plants should be included in regional reforestation projects in order to improve management of this bee species and honey production.
VANESSA R. MATOS
Full Text Available Twenty-two propolis samples produced by Apis mellifera L. in an area of the Semiarid region the the State of Bahia (Agreste of Alagoinhas, Brazil, were palynologically analyzed and quantified regarding their levels of total phenolic compounds. These samples were processed using the acetolysis technique with the changes suggested for use with propolis. We found 59 pollen types belonging to 19 families and 36 genera. The family Fabaceae was the most representative in this study with nine pollen types, followed by the family Asteraceae with seven types. The types Mikania and Mimosa pudica occurred in all samples analyzed. The types Mimosa pudica and Eucalyptus had frequency of occurrence above 50% in at least one sample. The highest similarity index (c. 72% occurred between the samples ER1 and ER2, belonging to the municipality of Entre Rios. Samples from the municipality of Inhambupe displayed the highest (36.78±1.52 mg/g EqAG and lowest (7.68 ± 2.58 mg/g EqAG levels of total phenolic compounds. Through the Spearman Correlation Coefficient we noticed that there was a negative linear correlation between the types Mimosa pudica (rs = -0.0419 and Eucalyptus (rs = -0.7090 with the profile of the levels of total phenolic compounds of the samples.
Teke, H.I.; Binzet, R.
In this study, the morphological, anatomical and palynological characters of Onosma discedens Hausskn. ex. Bornm., O. nana DC. and O. sorgerae var. subglabriflora Teppner were investigated. All the examined species are endemic to Anatolia, Turkey. The root cortex of examined species is multilayered. The raphide crystals are observed in epidermal cells and basal hair cells on both side of epidermis in O. sorgerae var. subglabriflora. Leaves are isobilateral (=equifacial) in O. nana and O. sorgerae var. subglabriflora, whereas leaves are dorsiventral(=bifacial) in O. discedens. Stomatas are observed on both surfaces (=amfistomatic) in all examined species. Stomata index is 9.25 of upper epidermis and 10.71 of lower epidermis in O. discedens, 9.61 of upper epidermis and 10 of lower epidermis in O. nana and 9.67 of upper epidermis and 13.63 of lower epidermis in O. sorgerae var. subglabriflora. The pollen grains are heteropolar, trisyncolporatae and sphaeroidal shape. In O. discedens, sphaeroidal (P/E: 1.13 W) and subprolate in Acetolysis and in O. nana, subprolate in shape and in O. sorgerae var. subglabriflora. Amb is three angular in all examined Onosma species. Sculpture is scabrate-rugulate in all species. Nutlet ornamentation is rugose. (author)
Mohd-Arrabe', A. B.; Noraini, Talip Noraini
Rhizophora L. are common mangrove genus in Peninsular Malaysia, it contains 3 species and 1 hybrid (R. apiculata Blume, R. mucronata Lam., R. stylosa Griff., R. x lamarckii Montrouz). This genus has some unique adaptation towards extreme environment. Rhizophora has looping aerial stilt-root and uniformly viviparous. The aim of this study is to investigate the variation in the pollen morphology of Rhizophora that can be related to their habitat. Methods include in this study is pollen observation under light and acetolysis method under scanning electron microscope. Pollen type of Rhizophora species studied except hybrid species is classified tricolporate, shape spheroidal based on ratio of length polar axis/ length of equatorial axis (1.03 - 1.09). The exine ornamentation is perforate-reticulate for R. apiculata and R. mucronata, while R. stylosa is perforate. For the only hybrid in Peninsular Malaysia, R. x lamarckii (R. apiculata x R. stylosa) differs from others, tricolpate with the absence of porate, shape is subprolate and exine ornamentation is reticulate and striate in equatorial region. Pollenkitt is present due to the salty and extreme environment. This may enhance the volume of pollenkitt present surrounding the pollen grains in Rhizophora for protection and adaptation purposes. Based on these findings, it is evident that pollen morphology is somehow related to its natural habitat
Full Text Available This study was carried out in the Parque Municipal das Araucárias, located in the municipality of Guarapuava (PR from May (06 to April (07. Its aim was to investigate which plants are used by Megachile (Moureapis sp in provisioning of larvae, and to verify if this bee is oligolectic or polilectic. The collection of the plants was carried out during the bees activity period and in an area covering a 500 m radius from where the nests had been built. A sample of pollens from founded nests and from flowers was used to make permanent slides using the acetolysis method and with the aid of a light microscope, all pollen grains were examined in order to identify the plants used by this bee. A total of 80 pollen grain slides, from 16 nests were analysed. Although 99 plants were collected close to the bee's nesting site, in nest slides, the pollen of Ludwigia peruviana (36% and Ludwigia sericea (63.6% (Onagraceae accounted for 99.6% of the total amount of pollen collected. With these results we are able to conclude that Megachile (Moureapis sp is an oligolectic species and that these plants species are their important pollen source.
Maria Selma Alves Silva Diamantino
Full Text Available The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L. and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%. For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate, magnesium sulfate and potassium nitrate. The pollen grains had the following characteristics: medium size, isopolar and subspheroidal shape, radial symmetry, circular ambit, 3-colporate, elongated endoapertures, tectate exine and granulated sexine. The acetocarmine dye overestimated pollen viability. The media M5 and M8 were the most efficient at promoting the germination of pollen grains. The studied genotypes had high levels of viability and can therefore be used as male parents in genetic improvement programs.
Fidalgo, Adriana de O; Kleinert, Astrid de M P
We describe the environment effects on the amount and quality of resources collected by Melipona rufiventris Lepeletier in the Atlantic Forest at Ubatuba city, São Paulo state, Brazil (44º48'W, 23º22'S). Bees carrying pollen and/or nectar were captured at nest entrances during 5 min every hour, from sunrise to sunset, once a month. Pollen loads were counted and saved for acetolysis. Nectar was collected, the volume was determined and the total dissolved solids were determined by refractometer. Air temperature, relative humidity and light intensity were also registered. The number of pollen loads reached its maximum value between 70% and 90% of relative humidity and 18ºC and 23ºC; for nectar loads this range was broader, 50-90% and 20-30ºC. The number of pollen loads increased as relative humidity rose (rs = 0.401; P < 0.01) and high temperatures had a strong negative influence on the number of pollen loads collected (rs = -0.228; P < 0.01). The number of nectar loads positively correlated with temperature (rs = 0.244; P < 0.01) and light intensity (rs = 0.414; P < 0.01). The percentage of total dissolved solids (TDS) on nectar loads positively correlated with temperature and light intensity (rs = 0.361; P < 0.01 and rs = 0.245; P < 0.01), negatively correlated with relative humidity (rs = -0.629; P < 0.01), and it increased along the day. Most nectar loads had TDS between 11% and 30%, with an average of 24.7%. The volume measures did not show any pattern. Important pollen sources were Sapindaceae, Anacardiaceae, Rubiaceae, Arecaceae, Solanaceae and Myrtaceae; nectar sources were Sapindaceae, Fabaceae, Rubiaceae, Arecaceae and Solanaceae.
Full Text Available The biological study of wild non-Apis bees can provide useful information that may help with the pollination of food crops and native plants in areas where the keeping of honey bee colonies is restricted or affected by CCD. Here, we describe the nesting biology of the Oriental large carpenter bee, Xylocopa (Biluna nasalis Westwood, 1838. An aggregation of more than 80+ bamboo nests of X. nasalis was discovered in Suan Pheung district, Ratch Buri province, Thailand on the 25th of May 2012. We collected 27 nests from the site to dissect, measure the external and internal nest architecture, and analyze the pollen composition of the pollen masses. X. nasalis constructs linear unbranched nests with nest entrance mostly located at the open-end of the bamboo culms. The nest length and the branch diameter of the nest entrance (excluding nesting edge are 25.40 ± 6.95 cm and 17.94 ± 6.00 mm, and the maximum number of provisioned cells is 8. A biased sex ratio of 8♀: 1♂ is reported, with up to 7 adults inhabiting in a single nest. 29 pollen types were identified from 14 pollen masses using an acetolysis method and visualization under both light microscope and scanning electron microscope. 13 pollen types were considered as major pollen sources (contribute ≥ 1% in total pollen volume; however, only 10 can be identified to family and generic levels. The dominant pollen sources are of the families Elaeagnaceae (Elaeagnus cf. latifolia, Euphorbiaceae (Croton, Fabaceae (Senna siamea and Cassia, Fagaceae (Lithocarpus and Castanopsis, and Lythraceae (Trapa which are mostly native to the region of Southeast Asia. The nesting architectural details should prove to be beneficial to beekeepers and researchers who are interested in trapping and studying X. nasalis, and the polylectic behavior of X. nasalis can be highly valuable for future crop pollination strategies, particularly for plants that require sonication of their poricidal anthers.
Maillet, Sebastien; Milhau, Bruno; Vreulx, Michel; Posada, Luis-Carlos Sánchez De
Asturian ostracods of the Givetian carbonate Candás Formation are documented for the first time from the Peran-Perlora and Carranques reference sections. More than 1,200 specimens were extracted from 44 samples by means of the hot acetolysis method. In all, 75 taxa are described herein, of which 21 are formally described and one, Evlanella peranensis Maillet n. sp., is new. All the taxa are marine benthic and belong to the Eifelian Mega-Assemblage. The assemblages recognized are representative of semi-restricted to shallow open-marine palaeoenvironments above the storm wave base. The stratigraphical distribution of the taxa shows a strong faunal renewal in the top of the Candás Formation. Long-ranging taxa found at the base of the formation, of which many are known from the base of the Middle Devonian, disappear within the base of the member C and are replaced above, around the Middle/Upper Givetian boundary, by more cosmopolitan taxa characteristic of the Frasnian. The lower half of the member C is also characterized both by unstable environments and occurrence of some short-ranging opportunistic ostracod taxa. This renewal within shallow water ostracod communities is probably a consequence of the global Taghanic Biocrisis, leading world-widely to extinctions in several faunal groups. Faunal affinities with Givetian ostracod taxa reported in other areas of the world reflect the commonly accepted palaeogeographical patterns. Close relations between the Cantabrian Zone (NW-Spain), the Armorican Massif (W-France), the Mouthoumet Massif (S-France) and North Africa (Morocco and Algeria) suggest a narrow oceanic space between the western European terranes and the northern Gondwanan margin that involves an advanced phase of closure of the Medio-European Ocean.
Marcos da Costa Dórea
Full Text Available This paper aims to identify the botanical origin of pollen loads collected by Apis mellifera L. in Canavieiras municipality, Bahia state. It provides a list of polliniferous plant species from the Atlantic Forest biome that are important for the development of regional apiculture. Using the acetolysis method, 35 bee-pollen samples were analyzed qualitatively and quantitatively. Results showed that pollen types Elaeis (23.99%, Mimosa pudica (22.78% and Cecropia (13.68% were the most abundant among the samples. These also showed the highest relative frequencies of the material studied and were important pollen sources for bees in the study area.O presente trabalho teve por objetivo identificar a origem botânica das bolotas de pólen coletadas pelas abelhas Apis mellifera L. no município de Canavieiras, Bahia, gerando uma lista de plantas poliníferas pertencentes ao bioma Mata Atlântica e importantes para o desenvolvimento da apicultura regional. Foram analisadas palinologicamente 35 amostras de pólen apícola, utilizando-se o processo de acetólise para, em seguida, serem feitas análises qualitativas e quantitativas. Os resultados revelaram que os tipos polínicos Elaeis (23,99%, Mimosa pudica (22,78% e Cecropia (13,68% foram os mais abundantes no pólen apícola analisado. Estes tipos polínicos também apresentaram as maiores frequências relativas de todo o material estudado e representam importantes plantas fontes de pólen para as abelhas na área estudada.
Kevin C. Matteson
Full Text Available Many cucurbits, such as cucumbers, squashes and pumpkins, depend on pollinating bees in order to set fruit. However, fruit yield and progeny vigor in these plants generally decreases as heterospecific pollen deposition increases. We studied how the spatial area dedicated to cucumbers (Cucumis sativis, versus other flowering plants, influenced the deposition of conspecific and heterospecific pollen on cucumber plants in New York City community gardens. We also examined the effect of garden size on conspecific and heterospecific pollen deposition on cucumber plants. Female flowers were collected from potted cucumber plants that had been experimentally placed into the gardens, specifically for this study, or that were established in raised beds by members of the community garden. In the laboratory, pollen grains were isolated from the flower by acetolysis, and the number of heterospecific and conspecific cucumber pollen grains were quantified. Conspecific pollen deposition was positively and significantly associated with the size of a community garden, as well as with the area of each garden dedicated to non-cucumber, flowering plants (i.e. floral cover and the area of each garden dedicated to cucumber plants (i.e. cucumber cover. Although floral cover explained a greater proportion of the variance, cucumber cover had the strongest effect on conspecific pollen deposition. Heterospecific pollen deposition was positively and significantly related to garden area. However, no significant relationship was found between heterospecific pollen deposition and floral cover, or cucumber cover. Based upon these results, we hypothesize that floral cover positively impacts conspecific pollen deposition by attracting a greater number of pollinators into an urban garden, and that total cucumber area positively impacts conspecific pollen deposition when pollinators are locally foraging within a garden. We suggest that the arrangement of plants within a garden can
Palinotaxonomia de espécies de Apocynaceae ocorrentes na Restinga de Carapebus, Carapebus, RJ, Brasil Palynotaxonomy of the species of the Apocynaceae occur on the "restinga" of the Carapebus, Carapebus, Rio de Janeiro State, Brazil
Fábio de França Moreira
Müll. Arg.; Tabernaemontana flavicans Willd. ex Roem. & Schult.; T. laeta Mart. The pollen grains were treated by the lactical acetolysis method, measured, described and illustrated by light microscopy and scanning electronic microscopy. The results obtained showed that two large groups can be identified: colporate pollen grains - Aspidosperma parvifolium (5-6-colporate, psilate exine, rugulate only apocolpus, Himatanthus lancifolius (3-colporate, reticulate exine, Tabernaemontana flavicans (4-colporate, endocingulate endoaperture and T. laeta (3-4-colporate, rugulate exine; porate pollen grains - Forsteronia leptocarpa, the four species of Mandevilla, Prestonia coalita, Rhabdadenia pohlii. It was concluded that the genera could be separated by pollinical characters and within genera their species studied are heterogeneous palynologically.
Pólen de gimnospermas e angiospermas em sedimentos quaternários de duas matas com Araucária, planalto leste do Rio Grande do Sul, Brasil Gymnosperm and angiosperm pollen in Quaternary sediments from two Araucaria forests on the Rio Grande do Sul State eastern plateau, Brazil
Full Text Available Catálogos polínicos de material contido em sedimentos servem como referência em estudos de reconstituição paleoambiental dos últimos milênios. Nesse contexto, a análise palinológica de dois perfis sedimentares do interior de matas com Araucária do estado do Rio Grande do Sul foi realizada. São apresentadas descrições dos grãos de pólen e dados ecológicos dos respectivos táxons de gimnospermas e angiospermas. A análise envolveu amostras de dois perfis sedimentares do Quaternário Tardio de São Francisco de Paula (perfil 1: Alpes de São Francisco, 29º29'S-50º37'W, perfil 2: Banhado Amarelo, 29º18'S-50º08'W, coletados com o Amostrador de Hiller. O processamento químico das amostras seguiu o método padrão, com HCl, HF, KOH, acetólise e montagem das lâminas em gelatina-glicerinada. A análise foi realizada em microscopia óptica. A descrição de cada material é acompanhada de ilustrações. São apresentados palinomorfos de três gimnospermas e 65 angiospermas. A grande riqueza do espectro polínico mostra a potencialidade deste material em oferecer informações ambientais relevantes no estudo da gênese e dinâmica da mata com Araucária.Sedimentary pollen catalogues are aids in the study of paleoenvironmental reconstruction of the last millennia. In this context, palynological analysis of two sedimentary profiles from inside Araucaria forests of Rio Grande do Sul state was done. Descriptions of gymnosperm and angiosperm pollen and ecological data of the respective taxa are presented. The analysis involved samples of two sedimentary profiles from the Late Quaternary of São Francisco de Paula (profile 1: Alpes de São Francisco, 29º29'S-50º37'W, profile 2: Banhado Amarelo, 29º18'S-50º08'W, collected with a Hiller Sampler. Chemical processing of the samples followed standard methodology, using HCl, HF, KOH, acetolysis and slide mounts in glycerol-jelly. The analysis was done by light microscopy. All material is
Geni da S. Sodré
Full Text Available Knowledge about the botanical source of honey is very important for the beekeeper while it indicates adequate and abundant supply sources of nectar and pollen for the bees, thus contributing toward improved yield. The present study means to identify the pollen types occurring in 58 samples of honey produced in two states of the northeastern region of Brazil, Piauí (38 samples and Ceará (20 samples, and to verify the potential of the honey plants during the months of February to August. The samples were obtained directly from beekeepers in each state and analyzed at the Apiculture Laboratory of the Entomology Section of Escola Superior de Agricultura "Luiz de Queiroz", USP, Piracicaba, State of São Paulo, Brazil. The pollen analysis was performed using the acetolysis method. The samples were submitted to both a qualitative and a quantitative analysis. The dominant pollen in the State of Ceará is from Mimosa caesalpiniaefolia, M. verrucosa, Borreria verticillata, Serjania sp., and a Fabaceae pollen type, while in the State of Piauí it is from Piptadenia sp., M. caesalpiniaefolia, M. verrucosa, Croton urucurana and Tibouchina sp.O conhecimento da origem botânica do mel é de grande importância para o apicultor por indicar fontes adequadas e de abundante suprimento de néctar e pólen para as abelhas, contribuindo, desta forma, para uma melhor produção. O presente estudo teve como objetivo identificar os tipos polínicos em 58 amostras de méis produzidos em dois estados da região nordeste do Brasil, Piauí (38 amostras e Ceará (20 amostras, verificando o potencial das plantas apícolas durante os meses de fevereiro a agosto. As amostras foram obtidas diretamente de apicultores de cada Estado e analisadas no Laboratório de Apicultura do Setor de Entomologia da Escola Superior de Agricultura "Luiz de Queiroz", USP, Piracicaba, Estado de São Paulo. A análise polínica foi realizada utilizando-se o método da acetólise. As amostras foram
Talebi, S. M.
Full Text Available Linum mucronatum is a heterostylous species from sect. Syllinum with four subspecies in Iran. The present study examines palynological characteristics of the heterostylous subspecies of Linum mucronatum, pollen characters of brevistylous individuals (pins as well as longistylous individuals (thrums of these plants by scanning electron microscope and light microscope using the prolonged acetolysis procedure. Sixteen qualitative and quantitative characters were investigated. Pollen equatorial shapes varied between pin and thrum individuals of each subspecies with the exception of L. mucronatum subsp. assyriacum. Pollen sculptures varied between pin and thrum samples of each subspecies and were seen in the gemmate, clavate and baculate shapes. In addition, quantitative palynological characters differed between plants and ANOVA test showed significant variations for traits such as equatorial length, colpi width and apocolpium diameter. Hetrostylous individuals of each subspecies were separated from others in the UPGMA tree and also in the PCO and PCA plots. This study confirmed variations in pollen features between pin and thrum individuals of each subspecies.Linum mucronatum es una especie con heterostilia, que pertenece a la sección Syllinum del género Linum, y tiene cuatro subespecies en Irán. En el presente estudio se examinan las características palinológicas de las subespecies heterostilas de Linum mucronatum Bertol., así como los caracteres polínicos de individuos de los morfos brevistilo (pin y longistilo (thrum de estas plantas, mediante microscopía electrónica de scanning y microscopía óptica usando el método de acetolisis prolongada. Se estudiaron un total de 16 caracteres cualitativos y cuantitativos. La forma ecuatorial del polen varía entre los morfos pin y thrum en todas las subspecies, excepto en L. mucronatum subsp. assyriacum. La ornamentación también varía entre las muestras de morfos pin y thrum de cada subespecie
Anna Frida Hatsue Modro
Full Text Available Este estudo objetivou identificar a origem botânica das cargas de pólen coletadas por abelhas africanizadas. Em Piracicaba (SP, foram instaladas, durante as quatro estações do ano, cinco colmeias de A. mellifera com um coletor de pólen frontal em cada uma. A preparação palinológica foi pelo uso de acetólise, sendo identificados e contados aproximadamente 900 grãos de pólen por amostra. A partir da composição polínica, calculou-se a riqueza, os índices de diversidade e equitabilidade. Ao longo do ano, foram encontrados 81 tipos polínicos, pertencentes a 32 famílias botânicas sendo Fabaceae, Asteraceae e Malvaceae, as famílias com maior frequência de tipos polínicos (≥5 tipos polínicos, e Myrtaceae, a família com dois tipos polínicos (Eucalyptus sp. e Myrcia sp. entre os nove mais frequentes nas amostras (>10%. A maior riqueza de tipos polínicos foi no verão e a maior diversidade e equitabilidade, na primavera. Fabaceae, Asteraceae, Malvaceae e Myrtaceae são as famílias botânicas mais importantes como fontes poliníferas em Piracicaba - SP.This study aimed to identify the botanic origin of pollen loads collected by Africanized honeybees. In Piracicaba (SP, during four seasons of the year, five honeybee colonies were installed with a frontal pollen collector in each one. The palinological preparation was done by the use of acetolysis and identified and counted approximately 900 pollen grains per sample. From the pollinic composition, the richness, diversity indexes and equitability were calculated. Throughout the year, 81 pollinic types were found, belonging to 32 botanic families, being Fabaceae, Asteraceae and Malvaceae the ones with the biggest frequency of pollinic types (≥5 pollinic types and, Myrtaceae, the family with two pollinic types (Eucalyptus sp. and Myrcia sp., among the nine most common types in the samples (> 10%. The biggest richness of pollinic types was during summer, and the biggest diversity and
Cargas polínicas de abelhas polinizadoras de Byrsonima chrysophylla Kunth. (Malpighiaceae: fidelidade e fontes alternativas de recursos florais Pollen loads of pollinator bees of Byrsonima chrysophylla Kunth. (Malpighiaceae: fidelity and alternative sources of flower resources
Éville Karina Maciel Delgado Ribeiro
Full Text Available Byrsonima chrysophylla, conhecida popularmente como murici pitanga, é uma espécie arbustiva que ocorre em restinga. Dentre seus visitantes, além de abelhas da tribo Centridini, também se encontram com freqüência abelhas Xylocopini. Para verificação das espécies vegetais utilizadas pelas abelhas visitantes mais freqüentes, cargas polínicas de suas pernas posteriores (n = 5, bem como de ninhos naturais de Centris caxiensis (n = 7 foram analisados através de acetólise. Durante toda a floração, Byrsonima chrysophylla foi a principal fonte de pólen utilizada por suas abelhas visitantes. Comolia lythrarioides e Myrcia obtusa foram fontes secundárias de pólen, Mouriri guianensis foi fonte secundária de óleo e pólen, enquanto Anacardium microcarpum, Cuphea tenella, Matayba discolor e Phthirusa pyrifolia mostraram-se importantes fontes de néctar durante esse período, sendo o pólen detectado nas amostras, provavelmente aderido ao corpo das abelhas durante a coleta de néctar. Os resultados demonstram que as abelhas visitantes não misturaram os recursos polínicos durante o vôo para a coleta de pólen, apresentando fidelidade às flores de Byrsonima chrysophylla.Byrsonima chrysophylla, known as "murici pitanga", is a shrubby species occurring in 'restinga'. Among its visitors, bees of the Centridini tribe as well as Xylocopini bees are frequently found. To detect which plant species have been used by the most frequent visitors, pollen loads from the back legs (n = 5 and also from natural nests of Centris caxiensis (n = 7 were treated by acetolysis. During flowering of Byrsonima chrysophylla, this plant species was the main pollen source used by bees. Comolia lythrarioides and Myrcia sylvatica were secondary pollen sources, Mouriri guianensis was a secondary oil and pollen source, while Anacardium microcarpum, Cuphea tenella, Matayba discolor and Phthirusa pyrifolia were important nectar sources at this time. The pollen detected in
André Rodrigo Rech
Full Text Available Bees and angiosperms established a mutualistic relationship along the evolutionary time. The aim of this study is to contribute for the understanding of this relation analyzing pollen stored by stingless bees colonies distributed along the Rio Negro. Fourteen species of Meliponini from the genera Partamona, Scaura, and Trigona were studied with regard to the content of pollen pots. The pollen material was removed from the pollen pots, homogenized, and prepared according to the usual acetolysis technique. The overlap of the trophic niche and the grouping of species by similarity of niches was calculated. The identification revealed 78 pollen types belonging to 36 families, being 37 types attractive and 16 considered as promoters of a temporary specialization event. With the results, it was possible to indicate a list of important plants for meliponiculture in the Amazon.Abelhas e plantas estabeleceram ao longo do tempo evolutivo uma relação mutualística. Buscando contribuir para o entendimento dessa relação, foi analisado o pólen estocado por colônias de abelhas-sem-ferrão distribuídas ao longo do rio Negro. Foram estudados potes de pólen de 14 espécies de Meliponini dos gêneros Partamona, Scaura e Trigona. O material polínico foi retirado dos potes de pólen, homogeneizado e preparado segundo técnica usual de acetólise. Foram calculados a sobreposição de nicho trófico e o agrupamento das espécies pela similaridade de nichos. Foi identificado o total de 78 tipos polínicos, pertencentes a 36 famílias, sendo 37 destes, considerados atrativos, enquanto 16 foram promotores de eventos de especialização temporária. Com os resultados obtidos foi possível indicar uma lista de plantas de importância para a meliponicultura na Amazônia.
Renata de Castro Nunes
Full Text Available Study and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P, equatorial diameter (E, exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM. All genotypes showed high rate of pollen viability (> 89% for both stains. There were differences between genotypes in the following quantitative traits: polar axis, equatorial diameter, exine, endexine, ektexine, and P/E ratio. Pollen grains were characterized as small, monoporates, with circular and non-prominent apertures. In addition to helping distinction of pollen grains, morphometric differences can be used later to compare ploidy levels, thus assisting in breeding programs of the species.O estudo e a caracterização do grão de pólen são fundamentais para diferentes áreas, em especial, para a taxonomia, o melhoramento genético, a filogenia e a paleobotânica. Para genótipos de Lolium multiflorum Lam., naturalizados no Brasil, cultivares introduzidas ou populações de melhoramento, diploides e poliploides, não existem relatos sobre a caracterização morfopolínica. Nesse trabalho foram analisados dez gentótipos de azevém anual (L. multiflorum. A viabilidade dos grãos de pólen foi verificada por coloração com os corantes carmim propiônico e Alexander e a morfologia por meio da técnica de acetólise, sendo obtidas medidas do eixo polar (P, do diâmetro equatorial (E, e da espessura da exina, e análise dos grãos de pólen por microscopia eletrônica de
Palinomorfos de fungos e criptógamas em sedimentos quaternários de duas matas com Araucária, Planalto leste do Rio Grande do Sul, Brasil Palynomorphs of fungi and cryptogams in Quaternary sediments from two Araucaria forests, Eastern Plateau of Rio Grande do Sul, Brazil
HCl, HF, KOH, acetolysis and slide mounts in glycerol-jelly. Light microscopy was used in the analysis. Palynomorphs of eight fungi, six algae, five bryophytes and 19 pteridophytes are presented. The description of each palynomorph is followed by illustrations and, whenever possible, by ecological data from the original organism. Distinct palynomorphs preserved in the sediments reveal the potential of this material in providing relevant environmental information, in association with pollen grains, in the study of genesis and expansion of the Araucaria forest.
Luiz Junior Pereira Marques
Full Text Available Foi realizado o levantamento das plantas visitadas por Apis mellifera L. através de análise polínica no município de Santa Luzia do Paruá, Maranhão, localizado a noroeste do Estado, em região amazônica. A temperatura média anual varia entre 26 °C e 27 °C, com umidade relativa do ar anual entre 79 a 82% e precipitação pluviométrica entre 2000 a 2400 mm anuais. O estudo foi conduzido entre agosto de 2005 e julho de 2006 em três apiários da região, com coletas mensais de mel, pólen e plantas, que foram submetidas ao método de acetólise. Os tipos polínicos das amostras de mel foram identificados e contados para a determinação da freqüência relativa média. Nas amostras de pólen foi realizada apenas a identificação dos tipos polínicos por meio da comparação com os polens das plantas (botões florais depositados em coleção de referência. As principais famílias utilizadas por A. mellifera foram Amaranthaceae, Arecaceae, Asteraceae, Urticaceae, Euphorbiaceae, Lamiaceae, Fabaceae-Mimosoideae, Flacourtiaceae, Myrtaceae, Poaceae e Rubiaceae. Em amostras de mel, no período chuvoso destacaram-se os tipos polínicos de Mimosa pudica, Borreria e um tipo indeterminado da família Arecaceae. No período de safra foram representativos Hyptis e um tipo indeterminado de Asteraceae. Os tipos polínicos mais constantes nas amostras de pólen foram Euterpe oleracea, Asteraceae tipo 1, Mimosa pudica e Borreria.The plant species visited by Apis mellifera L. were surveyed by pollen analysis in Santa Luzia do Paruá municipality, located in northwestern Maranhão state. Natural vegetation is Amazon rainforest, mean annual temperature varies between 26°C and 27°C, relative air humidity between 79 and 82%, and total rainfall between 2000 and 2400 mm. This study was conducted from August 2005 to July 2006 in three apiaries of the region, with monthly sampling of honey, pollen and plants. The samples were analyzed by the acetolysis method
Plant succession in a forest on the Lower Northeast Slope of Serra Geral, Rio Grande do Sul, and Holocene palaeoenvironments, Southern Brazil Sucessão vegetal em uma floresta da Encosta Inferior do Nordeste da Serra Geral, Rio Grande do Sul, e os paleoambientes do Holoceno, Sul do Brasil
Márcia Grala Leal
Full Text Available The study of plant succession provides information on vegetation dynamics of a region and, therefore, improves our understanding of the natural trends of present ecosystems. With this objective, plant succession based on palynology of a sediment profile in a swamp forest was studied on the Lower Northeast Slope of Serra Geral, Rio Grande do Sul. Samples were treated with HCl, HF, KOH and acetolysis; slides were mounted in glycerol-jelly. For each sample a minimum of 500 grains of pollen + pteridophyte and bryophyte spores was counted and other palynomorphs were counted in parallel. Tilia and Tilia Graph software were used to construct percentage and concentration diagrams, with three 14C datings. The results show a local water reservoir in the beginning of the Holocene (± 9800 yrs. BP, creating a herbaceous plant marsh by hydrosere. The initial colonization of the marsh by trees occurred about 8800 yrs. BP, with tropical species from the Coastal Plain migrating in an east-west direction. Afterwards, there was a dry phase between 7000-5000 yrs. BP. The second appearance of regional tree species began at about 5000 yrs. BP, resulting in the present forest and in the west-east migration of tropical species from interior lowlands, like this one, to re-colonize the coast after the last marine regression.O estudo de sucessão vegetal permite obter informações sobre a dinâmica da vegetação de uma região e, portanto, compreender melhor os ecossistemas da atualidade e suas tendências naturais. Com este objetivo foi feito um estudo de sucessão vegetal, com base em Palinologia, em um perfil sedimentar do interior de uma floresta paludosa, porção baixa da Encosta Inferior do Nordeste da Serra Geral, Rio Grande do Sul. As amostras foram tratadas com HCl, HF, KOH e acetólise e as lâminas montadas em gelatina-glicerinada. Para cada amostra foi contado um número mínimo de 500 grãos de pólen + esporos de pteridófitos e briófitos, com uma