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Sample records for acanthosis nigricans

  1. Acantosis Nigricans Maligna Acanthosis Nigricans Maligna

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    C Vallejo

    2011-06-01

    Full Text Available Es un síndrome paraneoplásico poco común, que se asocia a tumores gastrointestinales, más frecuentemente a adenocarcinoma gástrico (69%. Afecta a ambos sexos y no tiene prevalencia por grupo étnico alguno. Clínicamente son lesiones hiperpigmentadas, hipertróficas y verrucosas en zonas de flexión y en la mayoría de los casos, las mucosas también resultan afectadas con lesiones papilomatosas. Habitualmente desaparecen con la eliminación del tumor o reaparecen con la recurrencia o metástasis.Acanthosis nigricans malignant is an uncommon paraneoplastic syndrome associated with gastrointestinal tumors, frequently gastric adenocarcinoma, affects both sexes and it has not prevalence by race. Clinically, lesions are hyperpigmented, hypertrophic and verrucous in flexion areas and in most cases the mucous membranes are also affected with papillomatous lesions. Tipically disappears with the removal of the tumor and reappears when recurrence or metastasis occur.

  2. A case of acanthosis nigricans coexisting with mycosis fungoides.

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    Cheng, Esther; Roy, David B; Magro, Cynthia M

    2015-07-01

    Acanthosis nigricans maligna (ANM) is a paraneoplastic phenomenon most commonly associated with visceral adenocarcinoma, in particular, gastric adenocarcinoma. Clinically, AMN is characterized by rapidly progressive symmetrical skin thickening and hyperpigmentation of the intertriginous areas with peripheral acrochorda. The diagnosis is made by a detailed medical work-up for occult malignancies with particular emphasis on endocrinological diseases. We report a 67-year-old man that presented clinically with acanthosis nigricans, in which a subsequent diagnosis of mycosis fungoides was made.

  3. Oral malignant acanthosis nigricans associated with endometrial adenocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Han-Wen Chu; Jing-Min Li; Guan-Fu Chen; Jing-Yuan Ma

    2014-01-01

    We report a 59-year-old patient with malignant acanthosis nigricans associated with metastasis of endometrial carcinoma. The patient presented papillomatosis lesions that appeared to be benign on multiple skins of body folds, particularly on lips. The lesions in lips and axilla had histological characteristic appearances of acanthosis nigricans, while the masses in abdomen and pelvis were metastasis endometrial adenocarcinoma. The article highlights the importance of biopsy and histopathological diagnosis in presumed benign lesions and the role of doctors in screening for body internal tumors.

  4. Acanthosis Nigricans among Northern Plains American Indian Children

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    Brown, Blakely; Noonan, Curtis; Bentley, Bonnie; Conway, Kathrene; Corcoran, Mary; FourStar, Kris; Gress, Shannon; Wagner, Sharon

    2010-01-01

    The purpose of this study is to present cross-sectional and prospective data on acanthosis nigricans (AN) prevalence in the context of other risk factors for diabetes including high body mass index (BMI), abnormal blood pressure (BP), physical inactivity and family history of diabetes among Northern Plains American Indian (AI) children.…

  5. Observing Anthropometric and Acanthosis Nigrican Changes among Children Over Time

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    Law, Jennifer; Northrup, Karen; Wittberg, Richard; Lilly, Christa; Cottrell, Lesley

    2013-01-01

    This study assessed the anthropometrics and acanthosis nigricans (AN) in a sample of 7,337 children at two assessments. Four groups of children were identified based on the presence of AN at both time points: those who never had the marker, those who gained the marker, those who lost the marker, and those who maintained the marker. Group…

  6. Acral acanthosis nigricans in a case of scleroderma

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    Mahendra M Kura

    2015-01-01

    Full Text Available Acanthosis nigricans (AN is a dermatosis characterized by velvety, papillomatous, brownish-black, hyperkeratotic plaques, typically on the intertriginous surfaces and neck. The majority (80% of AN occurs idiopathically or in benign conditions such as endocrinopathies like diabetes mellitus, polycystic ovary syndrome; metabolic syndrome and/or heritable disease. Malignancy-associated AN is rare. AN may rarely be associated with autoimmune diseases including systemic lupus erythematosus, due to antibodies to the insulin receptor, so-called type B insulin resistance. Here we report a case of AN in a case of diffuse progressive systemic sclerosis without evidence of insulin resistance.

  7. Hyperandrogenism-Insulin Resistance-Acanthosis Nigricans Syndrome

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    A. H. Dédjan

    2015-01-01

    Full Text Available Introduction. Female hyperandrogenism is a frequent motive of consultation. It is revealed by hirsutism, acne or seborrhea, and disorders in menstruation cycle combined or not with virilisation signs. Several etiologies are incriminated but the hyperandrogenism-insulin resistance-acanthosis nigricans syndrome is rare. Observation. A 20-year-old girl, having had a five-year-old secondary amenorrhea. The exam revealed a patient, normotensive with a body mass index at 30 kg/m2 and a waist measurement of 120 cm, a severe hirsutism assessed to be 29 according to Ferriman Gallwey scale, virilisation signs of male morphotype, clitoridic hypertrophy and frontal alopecia, and an acanthosis nigricans behind the neck, in the armpits and elbows. The assessment carried out revealed testosteronemia at 1.28 ng/mL, which is more than twice the upper norm of the laboratory. Imaging studies were negative for both ovarian and adrenal masses. The retained diagnosis is HAIR-AN syndrome probably related to ovarian hyperthecosis and she was provided with androcur 50 mg/day and estradiol pills 2 mg/day and under hygiene-dietetic conditions. Conclusion. This case proves that HAIR-AN syndrome could be responsible for severe hyperandrogenism with virilisation signs. It must be retained after discarding the tumoral causes and when there are signs of insulin resistance.

  8. Acanthosis Nigricans associated with clear-cell renal cell carcinoma

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    Narvaez, Margarita Rosa Aveiga; Reis, Paola Vasconcellos Soares; Gomes, Augusto Cesar Marins; Paraskevopoulos, Daniela Kallíope de Sá; Santana, Frederico; Fugita, Oscar Eduardo Hidetoshi

    2016-01-01

    Acanthosis nigricans (AN), an entity recognized since the 19th century, is a dermatopathy associated with insulin-resistant conditions, endocrinopathies, drugs, chromosome abnormalities and neoplasia. The latter, also known as malignant AN, is mostly related to abdominal neoplasms. Malignant AN occurs frequently among elderly patients. In these cases, the onset is subtle, and spreading involves the flexural regions of the body, particularly the axillae, palms, soles, and mucosa. Gastric adenocarcinoma is the most frequent associated neoplasia, but many others have been reported. Renal cell carcinoma (RCC), although already reported, is rarely associated with malignant AN. The authors report the case of a woman who was being treated for depression but presented a long-standing and marked weight loss, followed by darkening of the neck and the axillary regions. Physical examination disclosed a tumoral mass in the left flank and symmetrical, pigmented, velvety, verrucous plaques on both axillae, which is classical for AN. The diagnostic work-up disclosed a huge renal mass, which was resected and further diagnosed as a RCC. The post-operative period was uneventful and the skin alteration was evanescent at the first follow-up consultation. The authors call attention to the association of AN with RCC. PMID:27284539

  9. A case of squamous cell carcinoma of lung presenting with paraneoplastic type of acanthosis nigricans

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    Subhasis Mukherjee

    2011-01-01

    Full Text Available A 70-years-old male presented with blackening of both hands and face for last six months which was progressive and attended dermatology outpatients department. Dermatologist opined the skin lesions as acanthosis nigricans. He was referred to our department to evaluate for any underlying internal malignancy as he was a smoker. His chest X-ray revealed right sided hilar prominence with a mid zone cavity with fluid level. Fibreoptic bronchoscopy was done, there was one ulcerative growth in right middle lobe bronchus. Biopsy from the ulcer revealed probable squamous cell carcinoma. CT scan of thorax was also done and CT guided FNAC of Rt lung lesion yielded non small cell carcinoma. His skin lesions were also biopsied and diagnosis of acanthosis nigricans was confirmed. Here we report a case of acanthosis nigricans associated with non-small cell cancer of lung.

  10. A Comparison of Blood Pressure, Body Mass Index, and Acanthosis Nigricans in School-Age Children

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    Otto, Debra E.; Wang, Xiaohui; Tijerina, Sandra L.; Reyna, Maria Elena; Farooqi, Mohammad I.; Shelton, Margarette L.

    2010-01-01

    The purpose of this retrospective quantitative study was to examine the relationships among acanthosis nigricans (AN), body mass index (BMI), blood pressure (BP), school grade, and gender in children attending elementary school located in South West Texas. Data were collected by attending school district nurses. Researchers reviewed 7,026…

  11. Hypochondroplasia, Acanthosis Nigricans, and Insulin Resistance in a Child with FGFR3 Mutation: Is It Just an Association?

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    Manal Mustafa

    2014-01-01

    Full Text Available FGFR3 mutations cause wide spectrum of disorders ranging from skeletal dysplasias (hypochondroplasia, achondroplasia, and thanatophoric dysplasia, benign skin tumors (epidermal nevi, seborrhaeic keratosis, and acanthosis nigricans, and epithelial malignancies (multiple myeloma and prostate and bladder carcinoma. Hypochondroplasia is the most common type of short-limb dwarfism in children resulting from fibroblast growth factor receptor 3 (FGFR3 mutation. Acanthosis nigricans might be seen in severe skeletal dysplasia, including thanatophoric dysplasia and SADDAN syndrome, without a biochemical evidence of hyperinsulinemia. Insulin insensitivity and acanthosis nigricans are uncommonly seen in hypochondroplasia patients with FGFR3 mutations which may represent a new association. We aim to describe the association of hypochondroplasia, acanthosis nigricans, and insulin resistance in a child harboring FGFR3 mutation. To our knowledge, this is the first case report associating the p.N540 with acanthosis nigricans and the second to describe hyperinsulinemia in hypochondroplasia. This finding demonstrates the possible coexistence of insulin insensitivity and acanthosis nigricans in hypochondroplasia patients.

  12. Acanthosis Nigricans Associated with an Adrenocortical Tumor in a Pediatric Patient

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    Elizabeth Isaacoff

    2013-01-01

    Full Text Available Malignant acanthosis nigricans (AN is a rare paraneoplastic syndrome seen primarily in adults with an underlying diagnosis of gastrointestinal adenocarcinoma. Malignant AN is characterized by hyperpigmentation and velvety hyperplasia of the epidermis. This condition is generally not associated with tumors in pediatric populations or in the adrenal gland. We present a case of malignant AN in a pediatric patient with a nonmalignant, functional adrenocortical tumor.

  13. A Longitudinal Study of Overweight, Elevated Blood Pressure, and Acanthosis Nigricans among Low-Income Middle School Students

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    Kopping, Dana; Nevarez, Holly; Goto, Keiko; Morgan, Irene; Frigaard, Martin; Wolff, Cindy

    2012-01-01

    This longitudinal study examined the rates of overweight, elevated blood pressure, acanthosis nigricans, and their associated factors in third through fifth grade students over 4 years. Participants consisted of 279 students who participated in health screenings in 2002 and 2006. Hispanic students had significantly higher rates of overweight and…

  14. Is Acanthosis Nigricans a Reliable Indicator for Risk of Type 2 Diabetes in Obese Children and Adolescents?: A Systematic Review

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    Abraham, Cilymol; Rozmus, Cathy L.

    2012-01-01

    Obesity and type 2 diabetes is becoming a major health problem affecting children and adolescents in the United States. This article reviews the current literature examining the association between the presence of acanthosis nigricans (AN) and risk for developing type 2 diabetes mellitus (T2DM) in obese children and adolescents. Ethnicity, family…

  15. Syndrome of insulin resistance with acanthosis nigricans, acral hypertrophy and muscle cramps in an adolescent - a rare diagnosis revisited.

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    Ghosh, Urmi; Thomas, Maya; Mathai, Sarah

    2014-12-01

    The authors report a 14-y-old boy with insulin resistance, acanthosis nigricans, acral hypertrophy and muscle cramps. While there was a dramatic response of the muscle cramps to phenytoin therapy, some other features of metabolic syndrome did not respond to phenytoin therapy alone.

  16. Acanthosis Nigricans

    Science.gov (United States)

    ... eds. Diabetes Mellitus and other endocrine diseases. In: Fitzpatrick's Dermatology in General Medicine. 8th ed. New York, ... eds. Cutaneous manifestations of internal malignant disease. In: Fitzpatrick's Dermatology in General Medicine. 8th ed. New York, ...

  17. Hyperandrogenism-insulin resistance-acanthosis nigricans syndrome with PCOS and Hashimoto's thyroiditis: case report

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    Amarnath Kulkarni

    2015-09-01

    Full Text Available Female hyperandrogenism is a frequent motive of consultation. It is revealed by hirsutism, acne or seborrhea, and disorders in menstruation cycle combined or not with virilisation signs. Several etiologies are incriminated but the hyperandrogenism-insulin resistance-acanthosis nigricans syndrome is rare. A 21-year female, having had a three-year-old secondary amenorrhea, known case of hypothyroidism since 4 years on medication. The exam revealed a patient, hypertensive with blood pressure at 170/110 mmHg with a Body Mass Index (BMI at 40.08 (Obese Class-3, as per WHO 2004 and a waist measurement of 106cm, a severe hirsutism assessed to be 27 according to Ferriman and Gallwey scale, acanthosis nigricans behind the neck and elbows. The assessment carried out revealed testosteronemia at 1.07 ng/mL, which is more than twice the upper normal of the laboratory. Imaging studies revealed enlarged right adrenal gland, hepatomegaly with fatty infiltration of grade-1 also bilateral polycystic ovaries. The retained diagnosis is HAIR-AN syndrome with polycystic ovaries, hypertension, type-II diabetes mellitus, hypothyroidism since last 4-years and dyslipidemia and was provided with metformin 500 mg thrice daily, spironolactone 25 mg twice daily, atorvastatin 20 mg once daily, telmisartan 20 mg once daily with continuation of eltroxin 50 Mcg for hypothyroidism. To our knowledge this is the first case report of HAIR-AN syndrome in 21 year old female associated with Hashimoto's thyroiditis, dyslipidaemia, hypertension and type-2-diabetes and this case also highlights about early diagnosis and management of HAIR-AN Syndrome with PCOS and Hashimoto's thyroiditis which could help prevent long-term sequalae such as cardiovascular disease and endometrial cancer and with the advent of knowledge and availability of health resources we can prevent long-term adverse effects (threefold on health of women. This woman should be observed for these ailments in later life

  18. Social Anxiety, Depression and Self-Esteem in Obese Adolescent Girls with Acanthosis Nigricans

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    Pirgon, Özgür; Sandal, Gonca; Gökçen, Cem; Bilgin, Hüseyin; Dündar, Bumin

    2015-01-01

    Objective: To assess the impact of acanthosis nigricans (AN) on depression symptoms, related quality of life and self-esteem scores in obese adolescent girls. Methods: Fifty-nine obese adolescent girls (mean age: 13.19±1.3 years, age range: 12-17 years, mean body mass index: 29.89±3.30) were enrolled in this study. The obese adolescent girls were divided into two groups based on presence or absence of AN. Non-obese healthy adolescents constituted the control group (30 girls, mean age: 13.5±1.4 years). All subjects were evaluated using the Children’s Depression Inventory (CDI), the State-Trait Anxiety Inventory for Children (STAI-C), and the modified Rosenberg Self-Esteem Scale (SES). Higher scores indicated more severe depression and anxiety, as well as low self-esteem status. Results: The AN and non-AN obese groups showed significantly higher CDI, STAI-C and SES scores than the control group, and the two obese groups demonstrated no significant differences for these scores. The AN obese group with higher total testosterone levels (>50 ng/dL) had higher scores for SES (2.55±1.8 vs. 1.42±1.2; p=0.03) than the AN obese group with low total testosterone levels. SES scores significantly correlated with total testosterone levels (r=0.362; p=0.03) and fasting insulin (r=0.462; p=0.03) in the AN obese group. Conclusion: Higher SES scores (low self-esteem status) were determined in obese adolescents with acanthosis and were related to hyperandrogenism. This study also showed that a high testosterone level may be one of the important indicators of low self-esteem status in obese girls with AN. PMID:25800478

  19. The Associations of Serum Uric Acid with Obesity-Related Acanthosis nigricans and Related Metabolic Indices

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    Rampersad, Sharvan; You, Hui; Sheng, Chunjun; Yang, Peng; Cheng, Xiaoyun; Bu, Le

    2017-01-01

    Objective. Recent studies have shown that hyperuricemia (HUA) is associated with hypertension, dyslipidemia, insulin resistance, and metabolic syndrome (MetS). We aimed to examine the relationship of serum UA with Acanthosis nigricans (AN) and related metabolic indices in obese patients. Methods. A cross-sectional study with 411 obese patients recruited from our department was analyzed in this study. Weight, body mass index (BMI), UA, lipid profile, liver function, and renal function were measured in all participants. Oral glucose tolerance tests were performed, and serum glucose, insulin, and C peptide were measured at 0, 30, 60, 120, and 180 min. Results. AN group had higher serum UA levels than OB group. Circulating UA levels were associated with BMI, dyslipidemia, hypertension, IR, and AN. In logistic regression analyses (multivariable‐adjusted), a high serum UA level was associated with high odds ratios (ORs) (95% confidence interval [CI]) for AN in females (ORs = 3.00 and 95% CI [1.02–8.84]) and males (ORs = 6.07 and 95% CI [2.16–17.06]) in the highest quartile (Q4) of serum UA. Conclusions. Serum UA levels were positively associated with multiple metabolic abnormalities including obesity, hypertension, hyperglycemia, hyperlipidemia, and AN and may be an important risk factor in the development of AN; further evidences in vitro and in vivo are needed to investigate the direct or indirect relationship.

  20. Quantitative skin color measurements in acanthosis nigricans patients: colorimetry and diffuse reflectance spectroscopy.

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    Pattamadilok, Bensachee; Devpura, Suneetha; Syed, Zain U; Agbai, Oma N; Vemulapalli, Pranita; Henderson, Marsha; Rehse, Steven J; Mahmoud, Bassel H; Lim, Henry W; Naik, Ratna; Hamzavi, Iltefat H

    2012-08-01

    Tristimulus colorimetry and diffuse reflectance spectroscopy (DRS) are white-light skin reflectance techniques used to measure the intensity of skin pigmentation. The tristimulus colorimeter is an instrument that measures a perceived color and the DRS instrument measures biological chromophores of the skin, including oxy- and deoxyhemoglobin, melanin and scattering. Data gathered from these tools can be used to understand morphological changes induced in skin chromophores due to conditions of the skin or their treatments. The purpose of this study was to evaluate the use of these two instruments in color measurements of acanthosis nigricans (AN) lesions. Eight patients with hyperinsulinemia and clinically diagnosable AN were seen monthly. Skin pigmentation was measured at three sites: the inner forearm, the medial aspect of the posterior neck, and anterior neck unaffected by AN. Of the three, measured tristimulus L*a*b* color parameters, the luminosity parameter L* was found to most reliably distinguish lesion from normally pigmented skin. The DRS instrument was able to characterize a lesion on the basis of the calculated melanin concentration, though melanin is a weak indicator of skin change and not a reliable measure to be used independently. Calculated oxyhemoglobin and deoxyhemoglobin concentrations were not found to be reliable indicators of AN. Tristimulus colorimetry may provide reliable methods for respectively quantifying and characterizing the objective color change in AN, while DRS may be useful in characterizing changes in skin melanin content associated with this skin condition.

  1. Critical comparison of diffuse reflectance spectroscopy and colorimetry as dermatological diagnostic tools for acanthosis nigricans: a chemometric approach.

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    Devpura, Suneetha; Pattamadilok, Bensachee; Syed, Zain U; Vemulapalli, Pranita; Henderson, Marsha; Rehse, Steven J; Hamzavi, Iltefat; Lim, Henry W; Naik, Ratna

    2011-06-01

    Quantification of skin changes due to acanthosis nigricans (AN), a disorder common among insulin-resistant diabetic and obese individuals, was investigated using two optical techniques: diffuse reflectance spectroscopy (DRS) and colorimetry. Measurements were obtained from AN lesions on the neck and two control sites of eight AN patients. A principal component/discriminant function analysis successfully differentiated between AN lesion and normal skin with 87.7% sensitivity and 94.8% specificity in DRS measurements and 97.2% sensitivity and 96.4% specificity in colorimetry measurements.

  2. Prenatal and postnatal presentation of severe achondroplasia with developmental delay and acanthosis nigricans (SADDAN) due to the FGFR3 Lys650Met mutation.

    NARCIS (Netherlands)

    Zankl, A.; Elakis, G.; Susman, R.D.; Inglis, G.; Gardener, G.; Buckley, M.F.; Roscioli, T.

    2008-01-01

    We present prenatal and postnatal features of a patient with severe achondroplasia with developmental delay and acanthosis nigricans (SADDAN). Mutation analysis confirmed the clinical diagnosis by detecting the FGFR3 Lys650Met mutation. This case, one of only six with molecular analysis reported in

  3. Acanthosis nigricans and insulin resistance in overweight children and adolescents Acantose nigricans e resistência insulínica em crianças e adolescentes com excesso de peso

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    Caroline Evelin Nascimento Kluczynik

    2012-08-01

    Full Text Available BACKGROUND: Studies have suggested an association between the presence of acanthosis nigricans (AN and the development of diabetes. OBJECTIVE: To investigate the association between AN and insulin resistance (IR in overweight children and adolescents receiving care at the Center for Childhood Obesity, Campina Grande, PB. METHODS: This cross-sectional study was conducted between April 2009 and April 2010 including 194 individuals of 2 to 18 years of age receiving care within the Brazilian national health network. The presence of acanthosis nigricans was verified and anthropometric measurements were taken. The following tests were performed: insulin, triglycerides, HDL-cholesterol, glucose and homeostasis model of assessment - insulin resistance (HOMA-IR. Statistical analyses were performed using the SPSS software program, version 17.0. RESULTS: There was a greater prevalence of females (66%, brown-skinned individuals (63.4%, adolescents (61.3% and severely obese individuals (66.5%. Acanthosis nigricans was identified in 58.2% and IR in 42.7% of the participants. Acanthosis nigricans was associated with being non-white (p = 0.003, with being an adolescent (p = 0.003 and with IR (p = 0.001. Non-white individuals, adolescents and those with insulin resistance were 5.4, 2.47 and 2.66 times more likely to have acanthosis nigricans, respectively. CONCLUSION: The results of this study indicate a need to train healthcare professionals to identify acanthosis nigricans, since this condition is associated with IR. Identifying acanthosis nigricans in childhood permits the safe and timely treatment of cardiometabolic disorders through careful monitoring and appropriate treatment.FUNDAMENTOS: Estudos sugerem haver associação entre a presença de Acantose Nigricans e o desenvolvimento do diabetes. OBJETIVO: Verificar a associação entre Acantose Nigricans e Resistência Insulínica (RI em crianças e adolescentes com excesso de peso, atendidos no Centro de

  4. Acanthosis nigricans as an indicator of insulin resistance in Chilean adult population Acantosis nigricans como un indicador de insulinoresistencia en población chilena adulta

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    A.C. Pinheiro

    2011-10-01

    Full Text Available Background: Insulin resistance (IR is associated with a higher risk of multiple diseases and its early detection would allow to minimize the associated risk; the presence of acanthosis nigricans (AN it´s associated to the presence of IR. Objective: To evaluate the sensibility and specificity of AN to diagnose IR in a group of Chilean patients. Methods: We designed a cross-sectional study and it was included subjects that were attended at the Center for the Attention of Metabolic Diseases at the Faculty of Medicine, University of Chile. Sixty subjects (18-60 years age were included. It was determined BMI and diagnosed AN and skin phototype; blood samples were taken and calculated the HOMA-IR. The normality of the variables where analyzed by Kolmogorov-Smirnov test. There were used χ² and the diagnostic concordance between AN and IR was determined using the Kappa index and Pearson´s correlation. Sensibility, specificity, positive and negative predictive value were calculated and accepted p Introducción: La presencia de resistencia a la insulina (RI se asocia a un mayor riesgo de padecer diversas enfermedades y su detección precoz permitiría minimizar el riesgo asociado; la presencia de acantosis nigricans (AN se asocia a la presencia de RI. Objetivo: Evaluar la sensibilidad y especificidad de la AN para diagnosticar RI en un grupo de pacientes chilenos. Método: Se realizó un estudio transversal y fueron incluidos sujetos atendidos por el Centro para Atención de Enfermedades Metabólicas, Facultad de Medicina, Universidad de Chile. En sesenta sujetos (18-60 años fueron determinados IMC, presencia de AN y fototipo de piel; muestras de plasma fueron tomadas para el cálculo del índice HOMA-IR. Fue analizada la normalidad de las variables con el test de Kolmogorov-Smirnov y utilizado χ² ; la concordancia diagnóstica entre AN y RI fue determinada con índice Kappa y correlación de Pearson. Fueron calculados sensibilidad, especificidad

  5. Acanthosis nigricans em mulheres obesas de uma população miscigenada: um marcador de distúrbios metabólicos Acanthosis nigricans in obese women in a mixed-race population: a marker of metabolic disturbances

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    Leila Maria Batista Araújo

    2002-10-01

    Full Text Available FUNDAMENTOS:Acanthosis nigricans (AN tem sido associada a diversos distúrbios metabólicos e endócrinos. OBJETIVOS: O objetivo deste estudo é avaliar a freqüência das co-morbidades da síndrome metabólica em mulheres obesas de uma população miscigenada com AN, comparada a um grupo sem AN. CASUÍSTICA E MÉTODOS: Foram estudadas 481 mulheres, consecutivamente admitidas em um ambulatório de obesidade (388 com AN e 93 sem AN e submetidas ao teste de tolerância à glicose oral, excetuando-se 20, que já se sabiam diabéticas. RESULTADOS: A distribuição segundo a raça indicou 34,5% de brancas, 38,9% de pardas e 26,6% de negras. A freqüência global de AN foi de 80,7%, sendo fortemente maior nas negras versus brancas (90,6 % e 66,9%, p=0,000000 e negras versus pardas (86% e 90,6%, p= 0,000006. Foi também maior nas pardas versus brancas (86% e 66,9%, pBACKGROUND: Acanthosis nigricans (AN has been associated with various metabolic and endocrine disturbances. OBJECTIVE: TThe objective of this study is to evaluate the frequency of metabolic syndrome clusters in a group of obese mixed-race women with AN as compared to a group without AN. SUBJECTS AND METHODS: 481 women consecutively admitted to an outpatient obesity clinic were studied: 388 with AN, and 93 without AN. Except for 20 diabetic patients, all patients were submitted to an oral glucose tolerance test (75 g. RESULTS: The skin color distribution was 34.5% white, 38.9% mulatto and 26.6% black. The global frequency of AN was 80.7%. AN frequency was significantly higher in black versus white and black versus mulatto (90.6 % and 66.9%, p = 0.000006. It was also higher in mulatto versus white (86% and 66.9%, p<0,02. The AN group was younger (35 + 10 years versus 38 + 10 years, p < 0,01 and heavier (41 + 6 kg/m² versus 39 + 6 kg/m², p<0.01. It consisted of larger waist circumference and higher frequency android obesity, with type 2 diabetes (11% versus 4.3%, p=0.05, higher fasting

  6. FGFR3 mutations and the skin: report of a patient with a FGFR3 gene mutation, acanthosis nigricans, hypochondroplasia and hyperinsulinemia and review of the literature

    DEFF Research Database (Denmark)

    Blomberg, M; Jeppesen, E M; Skovby, F

    2010-01-01

    developed acanthosis nigricans. The report of a K650Q mutation in the FGFR3 gene in a similar case prompted us to conduct a point mutation analysis. The K650Q mutation was confirmed, but in contrast to the previous case, we additionally report findings of hyperinsulinemia. In the recent literature...... keratoses and epidermal nevi, but also other benign skin tumors and a single case of a squamous cell carcinoma. In addition, an overview of the FGFR3 point mutations in relation to each cutaneous element is given. Based on the current knowledge, it seems likely that these cutaneous lesions have a common......Fibroblast growth factor receptor 3 (FGFR3) gene mutations in the germline are well-known causes of skeletal syndromes. Somatic FGFR3 mutations have been found in malignant neoplasms and more recently in several cutaneous elements. We present a 14-year-old girl with mild hypochondroplasia who...

  7. FGFR3 mutations and the skin: report of a patient with a FGFR3 gene mutation, acanthosis nigricans, hypochondroplasia and hyperinsulinemia and review of the literature

    DEFF Research Database (Denmark)

    Blomberg, M; Jeppesen, E M; Skovby, F;

    2010-01-01

    Fibroblast growth factor receptor 3 (FGFR3) gene mutations in the germline are well-known causes of skeletal syndromes. Somatic FGFR3 mutations have been found in malignant neoplasms and more recently in several cutaneous elements. We present a 14-year-old girl with mild hypochondroplasia who...... developed acanthosis nigricans. The report of a K650Q mutation in the FGFR3 gene in a similar case prompted us to conduct a point mutation analysis. The K650Q mutation was confirmed, but in contrast to the previous case, we additionally report findings of hyperinsulinemia. In the recent literature......, an increasing number of different cutaneous elements have been found to harbor mutations of FGFR3, suggesting that FGFR3 plays a role in the pathogenesis of these elements. We review the present literature, describing studies in which FGFR3 mutations have been investigated in skin lesions: primarily seborrheic...

  8. A descriptive study of facial acanthosis nigricans and its association with body mass index, waist circumference and insulin resistance using HOMA2 IR

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    Shyam Verma

    2016-01-01

    Full Text Available Introduction: The term facial acanthosis nigricans (FAN lacks definition of precise clinical and histopathological features. We present a descriptive study of patients with FAN to define pigmentary patterns and estimate the prevalence of obesity and insulin resistance in these cases. Materials and Methods: It is a prospective study that included all patients with classical AN of the neck and/or other areas with facial acanthosis nigricans described as brown-to-black macular pigmentation with blurred ill-defined margins, found on the zygomatic and malar areas. The body mass index (BMI and waist circumference (WC of the included patients were used as parameters of obesity.Homeostatic Model of Assessment of Insulin Resistance (HOMA2 IR was used as a parameter to evaluate insulin resistance. Histopathological features of the 6 skin biopsies that were possible were reviewed. Results: Among the 102 included individuals, the patterns of facial pigmentation seen in addition to the classic pattern involving zygomatic and malar areas were a hyperpigmented band on the forehead in 59.80%, periorbital darkening in 17.64%, perioral darkening in 12.74%, and generalized darkening in 9.8% of cases. 85.29% of the males and 100% of the females were found to be obese. Varying degrees of insulin resistance was noted in 82.34% of the individuals. Six biopsies available for evaluation showed changes such as mild epidermal hyperplasia with prominent basal melanin, however, without the typical papillomatosis seen in AN of the flexures. Conclusion: We document an increased prevalence of obesity and insulin resistance in patients presenting with FAN and its presentations in addition to the classical description. We propose that FAN can be considered a cutaneous marker of insulin resistance and that HOMA2 IR can serve as a parameter of insulin resistance in such cases.

  9. Association between acanthosis nigricans and metabolic syndrome in children with obesity%肥胖儿童黑色棘皮病与脂肪因子和代谢综合征的关系

    Institute of Scientific and Technical Information of China (English)

    谭新睿; 张美真; 李敏; 肖延风

    2015-01-01

    ObjectiveTo investigate adipokines levels in obese children with acanthosis nigricans (AN) and to explore the relationship between AN and metabolic syndrome (MS).MethodsA cross-sectional study was performed on 109 obese children and 47 age- and gender-matched normal controls. The obese children were divided into two groups with AN and without AN. Serum levels of adiponectin, leptin, TNF-α and retinol-binding protein 4 (RBP4) were measured using ELISA. Multiple logistic regression analysis was performed to estimate the association of clinical parameters with MS.ResultsWaist-hip ratio,systolic blood pressure, triglyceride, fasting insulin and insulin resistance index (HOMA-IR) were signiifcantly higher in obese children with AN than in those without AN and normal controls (P<0.05). The obese children with AN and without AN had lower adiponectin levels than normal controls (P<0.05), on the contrary, the obese children with AN had higher leptin levels than those without AN and normal controls (P<0.05). Multiple logistic regression analysis revealed that AN (OR=3.469, 95%CI: 1.518-7.929) and BMI (OR=7.108, 95%CI:2.359-21.416) were independent risk factors for MS.ConclusionsAs a visible marker of insulin resistance, AN is associated with abnormal adipokines secretion. Reducing the incidence of AN and losing weight may prevent obesity associated MS.%目的:了解肥胖伴黑色棘皮病(AN)患儿代谢特点及血清脂肪因子水平,探讨AN对代谢综合征的预测价值。方法将2012年2月至2013年6月收治的109名肥胖儿童分为伴AN组(n=44)和无AN组(n=65),同期选取年龄、性别相匹配的健康儿童做为对照组(n=47)。采用ELISA法检测血脂联素、瘦素、肿瘤坏死因子α和视黄醇结合蛋白4的水平,并对代谢综合征的危险因素进行多元logistic回归分析。结果肥胖伴AN组腰臀比、收缩压、血甘油三酯、空腹胰岛素、胰岛素抵抗指数均较肥胖

  10. Clinical Study on Acanthosis Nigricans and Non-Alcoholic Fatty Liver Disease in Obese Children%肥胖儿童黑棘皮伴非酒精性脂肪性肝病的临床探析

    Institute of Scientific and Technical Information of China (English)

    金亚男; 杨箐岩; 刘戈力; 郑荣秀

    2012-01-01

    Objective: To explore the significance of detecting acanthosis nigricans (AN) and non-alcoholic fatty liver disease (NAFLD) in early diagnosis of metabolic disorders in obese children. Methods: According to the presence of AN and/ or NAFLD, 132 ohese children were divided into four groups: children with AN and NAFLD(group A), children with AN but without NAFLD (group B), children with NAFLD but without AN (group C) and children without AN and NAFLD (group D). The values of body mass index (BMI), waist circumference (WC), fasting plasma glucose (FPG), triglyceride (TG), high density lipoprotein (HDL), systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting insulin (FINS) and homeostasis model of insulin resistance (HOMA-IR) were detected and compared between 4 groups. The prevalence of metabolic syndrome (MS) were also compared between the four groups. Results: (1) The values of BMI, WC, FINS and HOMA-IR were significantly higher in group A than those of group B, group C and group D. The level of HDL was significantly lower in group A than that of B, C and D groups. The level of DBP was higher in group A than that of group C and group D. The levels of FPG and TG were significantly higher in group A than those of B and D groups (P < 0.05 or P < 0.01). (2) The level of DBP was significantly higher in group B compared with that of group D. The values of FPG and TG were significantly higher in group C than those of group D. The values of WC, FINS and HOMA-IR were significantly higher in group B and group C than those of group D(P < 0.05 or P < 0.01). (3) The detection rates of prevalence of MS were 19 (45.24%), 5(16.13%), 5(16.67%) and 0 in group A, group B, group C and group D, respectively. The differences were significant between gcoups(X2= 22.539.P < 0.001). Conclusion: We should pay more attention to the obese children with AN and NAFLD because of more serious metabolic disorders in them.%目的:探讨黑棘皮(AN)结合非酒精性脂肪性肝

  11. A case report of confluent and reticulated papillomatosis with acanthosis nigricans:easilly confused with pityriasis versicolor%易与花斑糠疹混淆的融合性网状乳头瘤病合并黑棘皮病1例

    Institute of Scientific and Technical Information of China (English)

    文京华; 李伯埙; 黄小平

    2011-01-01

    融合性网状乳头瘤病为一种少见的原因不明的皮肤病,不易诊断.多见于青少年,好发于躯干部和腋窝,为褐色鳞屑性斑片或斑块.临床易误诊而被延误治疗.报告1例融合性网状乳头瘤病合并黑棘皮病,曾被多次拟诊为"花斑糠疹",有较典型的临床表现和组织病理改变.口服及外用异维A酸治疗有效.%Confluent and reticulate papillomatosis (CRP) with unknown etiology is uncommonly happened and easilly misdiagnosed. CRP occurs predominantly in young adults and teenagers with brown scaling patches and plaques affected the upper trunk and axillae. A case of malignant confluent and reticulated papillomatosis with acanthosis nigricans is reported. The patient had typical clinical manifestations and histopathologic changes. Oral and topical administration of isotretinoin showed therapeutic efficiency.

  12. 对黑棘皮病家系胰岛素受体基因变异的研究%A Study on Exon 17 and 20 of the Insulin Receptor Gene Variations in Patients with Acanthosis Nigricans and Their Close Relatives

    Institute of Scientific and Technical Information of China (English)

    沈捷; 丁国宪; 陈家伟; 庄旻; 王华; 夏红; 马向华

    2003-01-01

    Objective: To explore the relationship between the insulin resistance and the defects or mutatwns or mutatwns in insulin receptor ( InsR)gene. Methods: Using the single-strand conformation polymorphism( SSCP ), mutations and polymorphisms were detected in nine patients with acanthosis nigricans (AN) and their first degree relatives in exon 17 and 20 of InsR gene. The polymorphisms and mutations were confirmed by DNA direct sequencing. Results: Fourteen variant SSCP patterns were detected. Direct sequencing revealed seven point mutations and six silent polymorphisms. Five of the mutations appeared not to be mentioned in the previous literature. These mutations were all locatedwithin the domain of tyrokinase in InsR. Conclusion: It seem to us that almost all the AN patients with severe insulin resistance in this study have mutations in InsR tyrokinase domain.%目的:为了解胰岛素抵抗产生机制.方法:对患有黑棘皮病的患者应用单链构型多态性(SSCP)和DNA直接测序手段,进行了胰岛素受体基因17、20外显子的检测工作.结果:在9个黑棘皮病家系中(9名病人23位一级亲),共14个异常SSCP图型出现,经直接测序确认,其中有7个是错义突变,6个为沉默多态性,在7个错义突变中,5个为国际上首次报道(已在第5届中日糖尿病会议及第16届国际糖尿病联合会大会报告)结论:胰岛素受体基因突变致胰岛素抵抗.

  13. 黑棘皮病与青少年肥胖%Acanthosis nigerians and adolescent obesity

    Institute of Scientific and Technical Information of China (English)

    张志佳; 曲伸

    2013-01-01

    黑棘皮病是青少年肥胖患者中常见的皮肤病变,是青少年肥胖患者体内严重胰岛素抵抗的皮肤标志.在肥胖患者中,高胰岛素血症、炎性反应因子、异常分泌的脂肪细胞因子可促进皮肤组织内的成纤维细胞及角蛋白细胞的增殖,从而促进黑棘皮病的发生.另外,基因多态性同样在这一过程中发挥重要作用.然而青少年肥胖患者黑棘皮病的治疗尚无特效方法,通过饮食、运动及药物改善内胰岛素敏感性可能有效.%Acanthosis nigricans is a common cutaneous disorder in adolescent patients with obesity,which is a cutaneous marker of severe insulin resistance in this population.In obese patients,hyperinsulinemia,inflammatory factors and abnormal secretion of adipocytokines can promote cellular proliferation of fibroblasts and keratinocytes in the cutaneous tissue,thereby facilitate the development of acanthosis nigricans.In addition,gene polymorphism also plays an important role in this process.However,there is no effective therapy of acanthosis nigricans in adolescent patients with obesity.Improvement of the insulin sensitivity through diet,sport and medication may have benefit effects on acanthosis nigricans.

  14. Isolamento reprodutivo entre Australorbis glabratus e A. nigricans Reproductive isolation between Australorbis glabratus and A. nigricans

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    W. Lobato Paraense

    1955-12-01

    Full Text Available Foram feitas provas de cruzamento entre espécimes de uma população de A. glabratus e de outra de A. nigricans, utilizando o fator de albinismo como mercador genético. O resultado dessas provas revelou completo isolamento reprodutivo entre ambas, permitindo separá-las como boas espécies de acôrdo com o critério biológico.Crossing experiments were made between specimens from one population of A. glabratus and another of A. nigricans, the factor of albinism having been used as genetic marker. The results of those experiments revealed a complete reproductive isolation between both population, thus indicating their specific distinctness according to the biological criterion.

  15. The response of filamentous fungus Rhizopus nigricans to flavonoids.

    Science.gov (United States)

    Slana, Marko; Zigon, Dušan; Makovec, Tomaž; Lenasi, Helena

    2011-08-01

    The saprophytic fungus Rhizopus nigricans constitutes a serious problem when thriving on gathered crops. The identification of any compounds, especially natural ones, that inhibit fungal growth, may therefore be important. During its life cycle, Rhizopus nigricans encounters many compounds, among them the flavonoids, plant secondary metabolites that are involved in plant defense against pathogenic microorganisms. Although not being a plant pathogen, Rhizopus nigricans may interact with these compounds in the same way as plant pathogens--in response to the fungitoxic effect of flavonoids the fungi transform them into less toxic metabolites. We have studied the interaction of R. nigricans with some flavonoids. Inhibition of hyphal spreading (from 3% to 100%) was observed by 300 μM flavones, flavanones and isoflavones, irrespective of their basic structure, oxidized or reduced C-ring, and orientation of the B-ring. However, a hydrophobic A-ring was important for the toxicity. R. nigricans transformed some of the flavonoids into glucosylated products. Recognition of substrates for glucosylating enzyme(s) did not correlate with their fungitoxic effect but depended exclusively on the presence of a free -OH group in the flavonoid A-ring and of a hydrophobic B-ring. Although the fungus produced glucosyltransferase constitutively, an additional amount of the enzyme was induced by the substrate flavonoid. Moreover, effective detoxification was shown to require the presence of glucose.

  16. Anti-anxiety Activity Studies of Various Extracts of Pulsatilla nigricans Stoerck

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    Sandeep Goyal

    2010-10-01

    Full Text Available Pulsatilla nigricans Stoerck (Ranunculaceae has been traditionally used in nervousness, sadness, mild restlessness and mental unrest. Yet, the plant has never been subjected to systematic biological investigation. Therefore, the present investigation has been designed to evaluate anti-anxiety activity of P. nigricans. In the present investigation, petroleum ether (60-80°C, chloroform, methanol, and water extracts of P. nigricans aerial parts were evaluated for anti-anxiety activity in mice using elevated plus-maze apparatus. Among all the extracts, only methanol extract exhibited significant anti-anxiety activity at the dose of 200 mg/kg with respect to control as well as standard (diazepam, 2 mg/kg. As phytochemical screening of methanol extract showed presence of polyphenols, i.e., flavonoids and tannins, thus, these constituents might be responsible for anxiolytic potential of P. nigricans.

  17. "Prevalencia de Acantosis Nigricans, Enfermedad Cardiovascular, Sindrome del Ovario Poliquistico en Pacientes con Acantosis Nigricans Acral (ANA). Área de Consulta Externa del Hospital Luis Vernaza en el Periodo Julio del 2010 hasta junio del 2011"

    OpenAIRE

    Navarro Moncayo, Ana María

    2012-01-01

    Objetivo: Determinar la prevalencia de acantosis nigricans, enfermedad cardiovascular, y síndrome de ovario poliquístico en pacientes con acantosis nigricans acral. Metodología: Estudio observacional, descriptivo, transversal, que incluyo 64 pacientes de cualquier sexo atendidos en el área de consulta externa de dermatología del hospital Luis Vernaza, en el periodo de Julio del 2010 hasta Junio del 2011, con acantosis nigricans acral, que se realizaron toda la batería de exámenes y a los c...

  18. Nursing care in childcare services: Acantose nigricans as a marker for metabolic risk

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    Caroline Evelin Nascimento Kluczynik Vieira

    2013-12-01

    Full Text Available OBJECTIVE: to analyze the association between the presence of Acantose nigricans and metabolic changes in overweight adolescents, so as to ascertain the relevance of the identification of this marker in the nursing consultation. METHOD: a cross-sectional study undertaken between April 2009 and April 2010 with 118 adolescents who were service users of the Center for Child Obesity in Campina Grande in the Brazilian State of Paraíba (PB. The presence of Acantose nigricans, and the subjects' anthropometric measurements, were investigated. The following exams were made: insulin, triglycerides, HDL-Cholesterol, Glucose and the homeostatic model of assessment (HOMA-IR. RESULTS: there was association between the presence of Acantose nigricans and participants with insulin resistance (p=0.008, metabolic syndrome (p=0.031, elevated triglycerides (p=0.045 and altered HDL (p=0.002. CONCLUSIONS: the suggestion is supported that the detection/identification of Acantose nigricans may be used in the nursing consultation as a tool for identifying overweight adolescents with greater risk of metabolic changes.

  19. Calcivirus (SMSV-5) infection in experimentally inoculated Opaleye fish (Girella nigricans).

    Science.gov (United States)

    Smith, A W; Skilling, D E; Prato, C M; Bray, H L

    1981-01-01

    At 15 degrees C, San Miguel sea lion virus infected fish (Girella nigricans), producing 10(7).6 TCID50 per gram of spleen, replicated in Vero cells (10(8) TCID50/gm) and retained viability after 14 days exposure to salt water (10(5) TCID50/ml dropped to 10(2).

  20. Natural growth, otolith shape and diet analyses of Odontesthes nigricans Richardson (Atherinopsidae) from southern Patagonia

    Science.gov (United States)

    Lattuca, M. E.; Lozano, I. E.; Brown, D. R.; Renzi, M.; Luizon, C. A.

    2015-12-01

    Age and growth, otolith shape and diet of Odontesthes nigricans were analysed in order to provide an insight into the life history of the species and furthermore, to assess their possible use as a tool for discriminating silverside populations from the South Atlantic Ocean (Punta María) and Beagle Channel waters (Varela Bay). The age and growth analysis was performed by counting daily increments and annual marks in sagittae otoliths. Length-at-age data of individuals Gompertz model (SLt = 6.22 exp 2.45 [1-exp (-0.02t)]), which provided an excellent description of the pattern of daily growth for O. nigricans juveniles from Varela Bay. The spawning period was also assessed through back-calculation of hatching dates and it extended from November to February. The count of annual marks in larger individuals identified 7 year classes (0+ to 6+) in Varela Bay and 6 year classes (0+ to 5+) in Punta María. The von Bertalanffy growth model explained more than 95% of the growth patterns observed in O. nigricans from Varela Bay (SLt = 245.49 [1 - exp -0.24(t+0.46)]) and Punta María (SLt = 345.09 [1 - exp -0.15(t+0.31)]). Particularly, k and SL∞ varied significantly between sampling sites; reaching Punta María a larger SL∞ value with a lower k. Otolith shape variation was also explored using elliptical Fourier analysis and it showed significant differences between Varela Bay and Punta María populations. Furthermore, gut content analysis characterized O. nigricans as an invertebrate predator, being benthic organisms the most important components of its diet, which also showed significant site dependence. The use of all these analyses contributed to a holistic approach which maximized the likelihood of correctly identifying both O. nigricans populations in the southernmost limit of the species distribution.

  1. Diet of the blue marlin, Makaira nigricans, off the south coast of Portugal

    OpenAIRE

    Veiga, P; Xavier, J. C.; Assis, C. A.; Erzini, Karim

    2011-01-01

    The blue marlin, Makaira nigricans (Istiophoridae), is a large, top predator with a worldwide distribution whose feeding ecology is still unknown in the northeast Atlantic. The stomach contents of 24 Atlantic blue marlin, caught by the Big Game fishing fleet (between 2007 and 2010) off the south coast of Portugal, were studied. All marlin fed exclusively on pelagic fish, with a total of 180 prey items recorded, belonging to 6 identified species. The most important family in the blue marlin di...

  2. Analysis of High Risk Factors for Metabolic Syndrome in Obese Children with Acanthosis Nigricans%肥胖伴黑棘皮病儿童代谢综合征的高危因素

    Institute of Scientific and Technical Information of China (English)

    杨凌; 苏慧敏; 周忠蜀; 张知新

    2008-01-01

    目的 探讨肥胖伴黑棘皮病儿童代谢综合征(MS)的高危因素.方法 2006年11月-2007年9月在本院儿科就诊25例肥胖伴黑棘皮病儿童(病例组).男15例,女10例;年龄8.4~16.0岁,平均10.6岁;体质量(72.11±17.66)kg;身高(155±14)cm.32例身高别体质量正常的健康儿童为健康对照组.男18例,女14例;年龄7.6~15.8岁,平均9.8岁.比较二组儿童体质量指数(BMI)、胆固醇(12HO)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、血压、空腹和葡萄糖耐量试验(OGTT)2 h血糖、胰岛素和稳态模型评估法胰岛素抵抗指数(HOMA-IR)的差异,并对所有儿童进行肝脏超声波检查.采用SPSS 12.0软件进行统计学分析.结果 病例组儿童BMI、TG、LDL-C、收缩压和舒张压均显著高于健康对照组(Pa0.05).病例组患儿中超声诊断脂肪肝发生率为84%,健康对照组儿童肝脏B超检查均未见异常.结论 肥胖伴黑棘皮病儿童BMI增加、胰岛素抵抗、血脂紊乱和血压增高是MS的危险因素,密切随访监测此类患儿有助于早期发现MS.积极治疗肥胖症,阻断儿童血糖、血脂代谢紊乱的发生,有助于减少儿童2型糖尿病和心血管疾病的危险性.

  3. Structure, histochemistry, ultrastructure and seasonal variations of the male prostatic complex in the black Myotis bat, Myotis nigricans (Chiroptera: Vespertilionidae).

    Science.gov (United States)

    Negrin, Ana C; Beguelini, Mateus R; Puga, Cintia C I; Christante, Caroline M; Bueno, Larissa M; Morielle-Versute, Eliana; Vilamaior, Patrícia S L; Taboga, Sebastião R

    2014-10-01

    Chiroptera are one of the most diverse orders of mammals and a unique group within Mammalia that posses a wide geographic distribution and considerable variability in reproductive strategies. The aims of the present study were to characterise the male prostatic complex of the bat Myotis nigricans (Vespertilionidae) and evaluate seasonal variations in the prostatic complex of M. nigricans specifically. Twenty-three sexually mature specimens (four sample groups: winter, spring, summer and autumn) were subjected to macroscopic, microscopic, morphometric and ultrastructural analyses. The reproductive accessory glands of M. nigricans were found to be composed of a multilobed complex associated with the urethra and a pair of inguinal bulbourethral glands. The complex was composed of three bilobed prostatic regions (ventral, dorsolateral and dorsal) with no ampullary gland and seminal vesicles. This pattern of lobulation is very similar to that described for the prostate of rodents; however, it differs from that of other mammals and even other families of bats (e.g. Phyllostomidae and Molossidae). Each prostatic region in M. nigricans has unique and distinctive characteristics, which synchronise to establish the main reproductive peak of the species in summer. The data also indicated an asynchrony in the activity of primary and secondary reproductive organs in the annual reproductive cycle of M. nigricans in São Paulo State, Brazil.

  4. A new species of Myotis from the Islas Tres Marias, Nayarit, Mexico, with comments on variation in Myotis nigricans

    Science.gov (United States)

    Bogan, Michael A.

    1978-01-01

    A new Myotis is described from the Islas Tres Marias, Nayarit, Mexico. the new species is distinct from related taxa n the adjacent Mexican mainland (M. californicus, M. leibii, and M. carteri), although most closely related to M. carteri as shown by univariate and canonical variates analyses. An analysis of six groups of M. nigricans from Middle and South America supports the elevation of M. nigricans carteri to specific status, confirms the distinctness of M. nigricus extremus, but fails to substantiate subspecific status for bats from Columbia and Ecuador, recent recognized as M. n. punensis.

  5. Biotransformations of Imbricatolic Acid by Aspergillus niger and Rhizopus nigricans Cultures

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    Cristina Theoduloz

    2007-05-01

    Full Text Available Microbial transformation of imbricatolic acid (1 by Aspergillus niger afforded 1α-hydroxyimbricatolic acid (2, while transformation with Rhizopus nigricans yielded 15-hydroxy-8,17-epoxylabdan-19-oic acid (3. When the diterpene 1 was added to a Cunninghamella echinulata culture, the main products were the microbial metabolites mycophenolic acid (4 and its 3-hydroxy derivative 5. All the structures were elucidated by spectroscopic methods. The cytotoxicity of these compounds towards human lung fibroblasts and AGS cells was assessed. While 4 and 5 showed low cytotoxicity, with IC50 values > 1000 μM against AGS cells and fibroblasts, 1α-hydroxyimbricatolic acid (2 presented moderate toxicity towards these targets, with IC50 values of 307 and 631 μM, respectively. The structure of 2 is presented for the first time.

  6. Isolamento do vírus rábico de morcego insetívoro Myotis nigricans Isolation of the rabies virus in a specimen of the insectiverous bat Myotis nigricans

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    Luzia F. A. Martorelli

    1995-04-01

    Full Text Available Relata-se um caso de infecção natural de raiva em morcego insetívoro Myotis nigricans, no Município de Ribeirão Pires, Grande São Paulo (Brasil. O diagnóstico realizou-se através das técnicas de imunofluorescência e inoculação intracerebral em camundongos, do tecido nervoso e da musculatura da região interescapular do morcego.The following is a case study of the natural infection by the rabies virus of an insectiverous bat belonging to the species Myotis nigricans in the municipality of Ribeirão Pires, Greater S. Paulo. Diagnosis was made by means of immunofluorescence and intracerebral innoculation of mice with nervous and intrascapular muscular tissues.

  7. Size and shape variability in the skull of Myotis nigricans (Schinz, 1821 (Chiroptera: Vespertilionidae from two geographic areas in Brazil

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    R. Bornholdt

    Full Text Available We present a quantitative analysis of sexual dimorphism and geographic variation in the skull of Myotis nigricans (Schinz, 1821 assessed by geometric morphometrics. Differences in size and shape of skulls were investigated using 30 landmarks plotted on two-dimensional images of lateral and ventral views. Results of geometric morphometrics revealed sexual dimorphism in the centroid size of the skull in both views. Females were larger than males. Nevertheless, there was no sexual dimorphism in skull shape of M. nigricans. Geographic variation was detected in size and shape of the skull. South Brazilian specimens were significantly larger than Ceará specimens only in the lateral view. Differences in skull shape were statistically significant in both views: specimens from South Brazil were brevirostri and presented a more expanded skull in the posterior region while Ceará specimens were longirostri and do not present any expansion in the brain case. Ecological factors for these phenomena are discussed in the text.

  8. Habitat Fragmentation in Arid Zones: A Case Study of Linaria nigricans Under Land Use Changes (SE Spain)

    Science.gov (United States)

    Peñas, Julio; Benito, Blas; Lorite, Juan; Ballesteros, Miguel; Cañadas, Eva María; Martinez-Ortega, Montserrat

    2011-07-01

    Habitat fragmentation due to human activities is one of the most important causes of biodiversity loss. In Mediterranean areas the species have co-evolved with traditional farming, which has recently been replaced for more severe and aggressive practices. We use a methodological approach that enables the evaluation of the impact that agriculture and land use changes have for the conservation of sensitive species. As model species, we selected Linaria nigricans, a critically endangered plant from arid and semiarid ecosystems in south-eastern Spain. A chronosequence of the evolution of the suitable habitat for the species over more than 50 years has been reconstructed and several geometrical fragmentation indices have been calculated. A new index called fragmentation cadence (FC) is proposed to quantify the historical evolution of habitat fragmentation regardless of the habitat size. The application of this index has provided objective forecasting of the changes of each remnant population of L. nigricans. The results indicate that greenhouses and construction activities (mainly for tourist purposes) exert a strong impact on the populations of this endangered species. The habitat depletion showed peaks that constitute the destruction of 85% of the initial area in only 20 years for some populations of L. nigricans. According to the forecast established by the model, a rapid extinction could take place and some populations may disappear as early as the year 2030. Fragmentation-cadence analysis can help identify population units of primary concern for its conservation, by means of the adoption of improved management and regulatory measures.

  9. Structure of polysaccharides from mycelium and culture medium of Phellinus nigricans using submerged fermentation

    Institute of Scientific and Technical Information of China (English)

    LI Xia; JIAO LiLi; ZHANG Xu; TIAN WenMin; CHEN Shan; ZHANG LiPing

    2008-01-01

    Two water-soluble polysaccharides, PNW1 and PNM1, were respectively isolated from the mycelium and its culture medium of Phellinus nigricans using submerged fermentation before determining their effects on inhibiting the growth of transplantable tumors in mice. The results of the pharmaceutical experiments showed that oral administration of PNW1 and PNM1 (at a dose of 400 mg/kg) inhibited the growth of tumor of mice-transplanted Sarcoma 180 in vivo. Moreover, a higher inhibition ratio of PNW1 (74.70%) was obtained as compared with PNM1 (55.84%). The averaged molecular weight of PNW1 and PNM1 was determined to be 33 and 29 kD, respectively. Both PNW1 and PNM1 were consisted of glu-cose, galactose, mannose, arabinose and fucose. The major structural features of PNW1 and PNM1 were elucidated using partial acid hydrolysis, periodate oxidation, Smith degradation,13C-NMR, me-thylation and GC-MS. On the basis of these results, the repeating units of PNW1 and PNM1 were estab-lished.

  10. Structure of polysaccharides from mycelium and culture medium of Phellinus nigricans using submerged fermentation

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Two water-soluble polysaccharides, PNW1 and PNM1, were respectively isolated from the mycelium and its culture medium of Phellinus nigricans using submerged fermentation before determining their effects on inhibiting the growth of transplantable tumors in mice. The results of the pharmaceutical experiments showed that oral administration of PNW1 and PNM1 (at a dose of 400 mg/kg) inhibited the growth of tumor of mice-transplanted Sarcoma 180 in vivo. Moreover, a higher inhibition ratio of PNW1 (74.70%) was obtained as compared with PNM1 (55.84%). The averaged molecular weight of PNW1 and PNM1 was determined to be 33 and 29 kD, respectively. Both PNW1 and PNM1 were consisted of glu- cose, galactose, mannose, arabinose and fucose. The major structural features of PNW1 and PNM1 were elucidated using partial acid hydrolysis, periodate oxidation, Smith degradation, 13C-NMR, me- thylation and GC-MS. On the basis of these results, the repeating units of PNW1 and PNM1 were estab- lished.

  11. Purification, partial characterization and antitumor effect of an exopolysaccharide from Rhizopus nigricans.

    Science.gov (United States)

    Yu, Wenqian; Chen, Guochuang; Zhang, Pengying; Chen, Kaoshan

    2016-01-01

    In this study, a homogeneous exopolysaccharide (EPS1-1) was purified from the fermentation broth of Rhizopus nigricans. EPS1-1 was composed of glucose, mannose, galactose and fructose in the molar ratio of 5.89:3.64:3.20:1.00 with weight average molecular weight of 9.7×10(3)g/mol. EPS1-1 could significantly inhibit proliferation of human colorectal carcinoma HCT-116 cells in vitro. EPS1-1 also induced S phase cell cycle arrest and increased sub-G0/G1 population, a hallmark of apoptosis. The results of morphological characterization and flow cytometry showed that EPS1-1 induced apoptotic cell death in HCT-116 cells. EPS1-1 caused dissipation of mitochondrial membrane potential, accumulation of reactive oxygen species, up-regulation of Bax and p53 mRNA expression and down-regulation of Bcl-2 mRNA expression, which suggested that mitochondrial pathway was involved in the EPS1-1-induced apoptosis. These findings bring new insights into the potential use of EPS1-1 as antitumor drug against human colorectal carcinoma.

  12. Ecohydrological analysis of a groundwater influenced blanket bog: occurrence of Schoenus nigricans in Roundstone Bog, Connemara, Ireland

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    A.P. Grootjans

    2016-04-01

    Full Text Available Since the late 1960s, the occurrence of Schoenus nigricans in Irish blanket bogs has been attributed to inputs of salt spray to the blanket bogs, due to their proximity to the coast and the predominant westerly winds from the Atlantic Ocean. To test this hypothesis we carried out an ecohydrological field study at a large blanket bog in the western part of Connemara, Ireland. We described peat profiles in two transects and sampled pore water from peat at different depths. The water samples were analysed and their macro-ionic composition was used to locate possible inputs of calcareous groundwater to the system. We found clear evidence for inflow of calcareous groundwater at various sites and depths. Inflow of rather base-rich groundwater was indicated by high values of electrical conductivity (EC, high contents of calcium and bicarbonate, and high pH of the pore water. The peat profiles contained macro-remains of reed (Phragmites australis, in most cases only in deeper layers of peat, but at one location throughout the profile. This is another indication that the blanket bog was a groundwater-fed fen for quite some time. We conclude that the occurrence of S. nigricans in the blanket bog studied could be well explained by the hypothesis that S. nigricans is a relic from former more base-rich conditions. Relatively high base saturation could have persisted due to the prevailing groundwater flow in the upper layers preventing decalcification or other loss of cations from the whole soil profile including the topsoil.

  13. Isolamento do vírus rábico de morcego insetívoro Myotis nigricans

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    Luzia F. A. Martorelli

    1995-04-01

    Full Text Available Relata-se um caso de infecção natural de raiva em morcego insetívoro Myotis nigricans, no Município de Ribeirão Pires, Grande São Paulo (Brasil. O diagnóstico realizou-se através das técnicas de imunofluorescência e inoculação intracerebral em camundongos, do tecido nervoso e da musculatura da região interescapular do morcego.

  14. Isolamento do vírus rábico de morcego insetívoro Myotis nigricans

    Directory of Open Access Journals (Sweden)

    Martorelli Luzia F. A.

    1995-01-01

    Full Text Available Relata-se um caso de infecção natural de raiva em morcego insetívoro Myotis nigricans, no Município de Ribeirão Pires, Grande São Paulo (Brasil. O diagnóstico realizou-se através das técnicas de imunofluorescência e inoculação intracerebral em camundongos, do tecido nervoso e da musculatura da região interescapular do morcego.

  15. The effect of high dilutions of Pulsatilla nigricans on the vigour of soybean seeds subjected to accelerated aging=O efeito de altas diluições de Pulsatilla nigricans no vigor de sementes de soja submetidas ao envelhecimento acelerado

    Directory of Open Access Journals (Sweden)

    Bruno Reis

    2012-04-01

    Full Text Available The aim of this study was to evaluate the effect of high dilutions of Pulsatilla nigricans in dinamisations 6, 12, 18, 24 and 30 CH on the vigour of soybean seeds subjected to accelerated aging. The experiment was conducted according to a randomised design with 6 treatments and 10 replicates. The treatments consisted of dinamisations 6, 12, 18, 24 and 30 CH and a distilled-water control. After the treatments, the seeds were subjected to accelerated aging (48h at 42ºC in a growth chamber (25 ± 2ºC. The study evaluated the germination, the length of primary roots and shoots, the fresh weight of roots and shoots and the enzymatic activity of peroxidase (POX-EC1.11.1.7. The variables were analysed by ANOVA, and the means were compared using the Scott-Knott test (p = 0.05. The germination and the fresh weight of roots and shoots of the seedlings treated with Pulsatilla nigricans were higher than the water control, except that CH 30 did not significantly increase the fresh weight of shoots. The dinamisations 6, 24 and 30 CH produced a lower primary root length compared with the control. The dinamisations 12, 18 and 30 CH yielded a greater length of shoots. The total length of seedlings was reduced by the high dilutions 6 and 24 CH.O objetivo do presente trabalho foi de avaliar o efeito de altas diluições de Pulsatilla nigricans nas dinamizações 6, 12, 18, 24 e 30 CH, no vigor de sementes de soja submetidas ao envelhecimento acelerado. O experimento foi conduzido em delineamento inteiramente casualizado com 6 tratamentos e 10 repetições. Os tratamentos foram constituídos das dinamizações 6, 12, 18, 24 e 30 CH e testemunha com água destilada. Após aplicação dos tratamentos, as sementes foram submetidas ao teste de envelhecimento acelerado (48h a 42ºC, em câmara de crescimento (25 ± 2ºC. Avaliou-se a germinação, o comprimento da raiz primária e da parte aérea, massa fresca da raiz e da parte aérea e atividade enzimática da

  16. Producción de un biofertilizante a partir de un aislamiento de Azotobacter nigricans obtenido en un cultivo de Stevia rebaudiana Bert

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    Daniel Borda-Molina

    2009-04-01

    Full Text Available Bio-fertilizer production from an isolate of Azotobacter nigricans obtained from a plantation of Stevia rebaudiana Bert. Objective.To isolate nitrogen fixing bacteria to be used in a fertilization regime of an organic agriculture program. Materials and methods. Theisolation of nitrogen fixing bacteria was done in an Ashby-benzoate medium from soil of a Stevia rebaudiana plantation. Isolates identifiedas Azotobacter nigricans were evaluated by their growth kinetics and the strain with the fastest growth was used for the production of abiofertilizer by discontinuous fermentation. The preliminary evaluation of the biofertilizer was done by its inoculation into t hree ridges ofa plantation of S. rebaudiana and yield determination was based upon biomass production and glycoside concentration. Results. Twoisolates (A5 and A6 were identified as A. nigricans based on their phenotypic and genotypic characterization. Isolate A5 was selected forpreparing the biofertilizer because it showed a better stability, pigmentation, a faster growth rate (0.1405 h-1 exponential phase of 18 hoursand an average IAA production of 38.4 mg/ml after 150 hours. The bio-fertilizer was obtained in milk medium with a cell concentration of4x1012 CFU/ml. Conclusions. The preliminary field evaluation showed a positive correlation between the increase of the glycosideconcentration in the leaves of S. rebaudiana and a higher production of biomass in response to the bio-fertilizer application.

  17. Impact of the processes of testicular regression and recrudescence in the prostatic complex of the bat Myotis nigricans (Chiroptera: Vespertilionidae).

    Science.gov (United States)

    Beguelini, Mateus R; Góes, Rejane M; Rahal, Paula; Morielle-Versute, Eliana; Taboga, Sebastião R

    2015-07-01

    Myotis nigricans is a species of vespertilionid bat, whose males show two periods of total testicular regression during the annual reproductive cycle in the northwest São Paulo State, Brazil. Thus, the aim of this study was to investigate the impact of total testicular regression on the prostatic morphophisyology and its regulation. The prostatic complex (PC) of animals from the four periods of the reproductive cycle (active, regressing, regressed, and recrudescence) was analyzed by different histological, morphometric, and immunohistochemical procedures to characterize its variations, analyze its hormonal regulation and evaluate whether the prostate is affected by the processes of testicular regression and recrudescence. The results indicated a decrease in the prostatic parameters from the active to regressed periods, which are related to decreases in the testicular production of testosterone and in the prostatic expression of androgen receptor (AR), estrogen receptor α (ERα) and aromatase. However, in regressed-recrudescence periods, the prostatic expression of AR, ERα and aromatase increased, indicating the reactivation of the PC. Despite this, the PC appears to have a slower reactivation and seems not to follow the testicular recrudescence in morphological and morphometric terms. With these data, we demonstrate that the prostatic physiology is directly affected by total testicular regression and conclude that it is regulated by testosterone and estrogen, via the production of testosterone by the testes, its conversion to dihydrotestosterone by 5α-redutase and to estrogen by aromatase, and the activation/deactivation of AR and ERα in epithelial cells, which regulate cell expression and proliferation.

  18. Lead Biosorption by Self-Immobilized Rhizopus nigricans Pellets in a Laboratory Scale Packed Bed Column: Mathematical Model and Experiment

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    Adela Kogej

    2010-01-01

    Full Text Available The biosorption of lead ions from aqueous solution on a self-immobilized Rhizopus nigricans biomass has been studied. Experiments were performed in a laboratory scale packed bed column at different liquid flow rates and biosorbent bed heights. Recorded experimental breakthrough curves were compared to those predicted by a mathematical model, which was developed to simulate a packed bed biosorption process by a soft, self-immobilized fungal biosorbent. In the range of examined experimental conditions, the biomass characteristics such as pellet porosity and biosorption capacity substantially affected the predicted response curve. General correlations for the estimation of the intra-pellet effective diffusivity, the external mass transfer coefficient, as well as axial dispersion were successfully applied in this biological system with specific mechanical properties. Under the experimental conditions, mass transfer is controlled by the external film resistance, while the intra-pellet mass transfer resistance, as well as the effect of axial dispersion, can be neglected. A new parameter α, the fraction of active biomass, with an average value of α=0.7, was introduced to take into account the specific biomass characteristics, and consequently the observed non-ideal liquid flow through the bed of fungal pellets.

  19. Reconciling patterns of inter-ocean molecular variance from four classes of molecular markers in blue marlin (Makaira nigricans).

    Science.gov (United States)

    Buonaccorsi, V P; McDowell, J R; Graves, J E

    2001-05-01

    Different classes of molecular markers occasionally yield discordant views of population structure within a species. Here, we examine the distribution of molecular variance from 14 polymorphic loci comprising four classes of molecular markers within approximately 400 blue marlin individuals (Makaira nigricans). Samples were collected from the Atlantic and Pacific Oceans over 5 years. Data from five hypervariable tetranucleotide microsatellite loci and restriction fragment length polymorphism (RFLP) analysis of whole molecule mitochondrial DNA (mtDNA) were reported and compared with previous analyses of allozyme and single-copy nuclear DNA (scnDNA) loci. Temporal variance in allele frequencies was nonsignificant in nearly all cases. Mitochondrial and microsatellite loci revealed striking phylogeographic partitioning among Atlantic and Pacific Ocean samples. A large cluster of alleles was present almost exclusively in Atlantic individuals at one microsatellite locus and for mtDNA, suggesting that, if gene flow occurs, it is likely to be unidirectional from Pacific to Atlantic oceans. Mitochondrial DNA inter-ocean divergence (FST) was almost four times greater than microsatellite or combined nuclear divergences including allozyme and scnDNA markers. Estimates of Neu varied by five orders of magnitude among marker classes. Using mathematical and computer simulation approaches, we show that substantially different distributions of FST are expected from marker classes that differ in mode of inheritance and rate of mutation, without influence of natural selection or sex-biased dispersal. Furthermore, divergent FST values can be reconciled by quantifying the balance between genetic drift, mutation and migration. These results illustrate the usefulness of a mitochondrial analysis of population history, and relative precision of nuclear estimates of gene flow based on a mean of several loci.

  20. Fidelity and breeding probability related to population density and individual quality in black brent geese Branta bernicla nigricans

    Science.gov (United States)

    Sedinger, J.S.; Chelgren, N.D.; Ward, D.H.; Lindberg, M.S.

    2008-01-01

    1. Patterns of temporary emigration (associated with non-breeding) are important components of variation in individual quality. Permanent emigration from the natal area has important implications for both individual fitness and local population dynamics. 2. We estimated both permanent and temporary emigration of black brent geese (Branta bernicla nigricans Lawrence) from the Tutakoke River colony, using observations of marked brent geese on breeding and wintering areas, and recoveries of ringed individuals by hunters. We used the likelihood developed by Lindberg, Kendall, Hines & Anderson 2001 (Combining band recovery data and Pollock's robust design to model temporary and permanent emigration. Biometrics, 57, 273-281) to assess hypotheses and estimate parameters. 3. Temporary emigration (the converse of breeding) varied among age classes up to age 5, and differed between individuals that bred in the previous years vs. those that did not. Consistent with the hypothesis of variation in individual quality, individuals with a higher probability of breeding in one year also had a higher probability of breeding the next year. 4. Natal fidelity of females ranged from 0.70 ?? 0.07-0.96 ?? 0.18 and averaged 0.83. In contrast to Lindberg et al. (1998), we did not detect a relationship between fidelity and local population density. Natal fidelity was negatively correlated with first-year survival, suggesting that competition among individuals of the same age for breeding territories influenced dispersal. Once females nested at the Tutakoke River, colony breeding fidelity was 1.0. 5. Our analyses show substantial variation in individual quality associated with fitness, which other analyses suggest is strongly influenced by early environment. Our analyses also suggest substantial interchange among breeding colonies of brent geese, as first shown by Lindberg et al. (1998).

  1. Survival and breeding advantages of larger Black Brant (Branta bernicla nigricans) goslings: Within- and among-cohort variation

    Science.gov (United States)

    Sedinger, J.S.; Chelgren, N.D.

    2007-01-01

    We examined the relationship between mass late in the first summer and survival and return to the natal breeding colony for 12 cohorts (1986-1997) of female Black Brant (Branta bernicla nigricans). We used Cormack-Jolly-Seber methods and the program MARK to analyze capture-recapture data. Models included two kinds of residuals from regressions of mass on days after peak of hatch when goslings were measured; one based on the entire sample (12 cohorts) and the other based only on individuals in the same cohort. Some models contained date of peak of hatch (a group covariate related to lateness of nesting in that year) and mean cohort residual mass. Finally, models allowed survival to vary among cohorts. The best model of encounter probability included an effect of residual mass on encounter probability and allowed encounter probability to vary among age classes and across years. All competitive models contained an effect of one of the estimates of residual mass; relatively larger goslings survived their first year at higher rates. Goslings in cohorts from later years in the analysis tended to have lower first-year survival, after controlling for residual mass, which reflected the generally smaller mean masses for these cohorts but was potentially also a result of population-density effects additional to those on growth. Variation among cohorts in mean mass accounted for 56% of variation among cohorts in first-year survival. Encounter probabilities, which were correlated with breeding probability, increased with relative mass, which suggests that larger goslings not only survived at higher rates but also bred at higher rates. Although our findings support the well-established linkage between gosling mass and fitness, they suggest that additional environmental factors also influence first-year survival. ?? The American Ornithologists' Union, 2007.

  2. Ultrastructural characteristics of the spermatogenesis during the four phases of the annual reproductive cycle of the black myotis bat, Myotis nigricans (Chiroptera: Vespertilionidae).

    Science.gov (United States)

    Beguelini, Mateus R; Taboga, Sebastião R; Morielle-Versute, Eliana

    2013-10-01

    Myotis nigricans is an endemic species of vespertilionid bat, from the Neotropical region, that resembles temperate zone bats in their reproductive cycle; presenting an annual reproductive cycle with two periods of testicular regression, which are not linked to the apoptotic process and seems to be not directly linked to any seasonal abiotic variation. Thus, this study aimed to ultrastructurally evaluate their reproductive cycle. The process of testicular regression could be divided into four periods: active; regressing; regressed and recrudescence; with all presenting distinct characteristics. The active period was similar to that of other bats, presenting the complete occurrence of spermatogenesis, with three main types of spermatogonia (A(d), A(p), and B) and 12 steps in spermatid differentiation; however, it differed in having the outer dense fibers 1, 5, 6, and 9 larger than the others. These three types of spermatogonia undergo considerable morphologic changes from regressing to the regressed period, and in the recrudescence, they return to the basic morphology, which reactivates spermatogenesis. In conclusion, our study described the process of spermatogenesis, the ultrastructure of the spermatozoa and the distinct morphologic variations in the ultrastructure of the testicular cells of M. nigricans during the four different periods of its annual reproductive cycle.

  3. Impact of the Processes of Total Testicular Regression and Recrudescence on the Epididymal Physiology of the Bat Myotis nigricans (Chiroptera: Vespertilionidae).

    Science.gov (United States)

    Beguelini, Mateus R; Góes, Rejane M; Rahal, Paula; Morielle-Versute, Eliana; Taboga, Sebastião R

    2015-01-01

    Myotis nigricans is a species of vespertilionid bat, whose males show two periods of total testicular regression within the same annual reproductive cycle in the northwest São Paulo State, Brazil. Studies have demonstrated that its epididymis has an elongation of the caudal portion, which stores spermatozoa during the period of testicular regression in July, but that they had no sperm during the regression in November. Thus, the aim of this study was to analyze the impact of the total testicular regression in the epididymal morphophysiology and patterns of its hormonal regulation. The results demonstrate a continuous activity of the epididymis from the Active to the Regressing periods; a morphofunctional regression of the epididymis in the Regressed period; and a slow recrudescence process. Thus, we concluded that the processes of total testicular regression and posterior recrudescence suffered by M. nigricans also impact the physiology of the epididymis, but with a delay in epididymal response. Epididymal physiology is regulated by testosterone and estrogen, through the production and secretion of testosterone by the testes, its conduction to the epididymis (mainly through luminal fluid), conversion of testosterone to dihydrotestosterone by the 5α-reductase enzyme (mainly in epithelial cells) and to estrogen by aromatase; and through the activation/deactivation of the androgen receptor and estrogen receptor α in epithelial cells, which regulate the epithelial cell morphophysiology, prevents cell death and regulates their protein expression and secretion, which ensures the maturation and storage of the spermatozoa.

  4. Impact of the Processes of Total Testicular Regression and Recrudescence on the Epididymal Physiology of the Bat Myotis nigricans (Chiroptera: Vespertilionidae.

    Directory of Open Access Journals (Sweden)

    Mateus R Beguelini

    Full Text Available Myotis nigricans is a species of vespertilionid bat, whose males show two periods of total testicular regression within the same annual reproductive cycle in the northwest São Paulo State, Brazil. Studies have demonstrated that its epididymis has an elongation of the caudal portion, which stores spermatozoa during the period of testicular regression in July, but that they had no sperm during the regression in November. Thus, the aim of this study was to analyze the impact of the total testicular regression in the epididymal morphophysiology and patterns of its hormonal regulation. The results demonstrate a continuous activity of the epididymis from the Active to the Regressing periods; a morphofunctional regression of the epididymis in the Regressed period; and a slow recrudescence process. Thus, we concluded that the processes of total testicular regression and posterior recrudescence suffered by M. nigricans also impact the physiology of the epididymis, but with a delay in epididymal response. Epididymal physiology is regulated by testosterone and estrogen, through the production and secretion of testosterone by the testes, its conduction to the epididymis (mainly through luminal fluid, conversion of testosterone to dihydrotestosterone by the 5α-reductase enzyme (mainly in epithelial cells and to estrogen by aromatase; and through the activation/deactivation of the androgen receptor and estrogen receptor α in epithelial cells, which regulate the epithelial cell morphophysiology, prevents cell death and regulates their protein expression and secretion, which ensures the maturation and storage of the spermatozoa.

  5. Reproductive and biochemical changes in obese and non obese polycystic ovary syndrome women

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    Manal Ibrahim Mahmoud

    2015-03-01

    Conclusions: Overweight and obese PCOS women had significantly higher age of menarche, abortion and menstrual disturbances. Also, signs of hyperandrogenism, acanthosis nigricans were more encountered among them with higher levels of fasting glucose, fasting insulin, FSH, LH and testosterone.

  6. Tinea Versicolor

    Science.gov (United States)

    ... partners Español Donate Diseases and treatments Acne and rosacea Bumps and growths Color problems Contagious skin diseases ... SPOT Skin Cancer™ Diseases and treatments Acne and rosacea Bumps and growths Color problems Acanthosis nigricans Melasma ...

  7. Androgen Excess Disorders in Women: The Severe Insulin-Resistant Hyperandrogenic Syndrome, HAIR-AN

    OpenAIRE

    Rager, Kristin M.; Hatim A. Omar

    2006-01-01

    HAIR-AN syndrome (hyperandrogenism, insulin resistance, acanthosis nigricans) is a subset of the polycystic ovary syndrome, where the patients demonstrate severe insulin resistance. It is theorized that both genetic and environmental factors, such as obesity, give rise to the development of HAIR-AN. Diagnosis is primarily clinical, with laboratory values lending further support. Treatment is aimed at decreasing insulin resistance, regulating ovulation, and decreasing acne, acanthosis nigrican...

  8. Documentation for the Computer Assisted Diagnostic Program for Dental Pain

    Science.gov (United States)

    1989-04-10

    palate) Amyloidosis *Sarcoidosis *Verruca vulgaris, mIultiple lesions Focal dermal hypcplasia syndrume Darier’s disease *Acanthosis nigricans * Crohn ’ s ...mouth) Mouth breathing *Diabetes (uncontrolled) *Weener s granulomatosis *Cyclic neutropenia *Cushing’s syrom *Yellow fever Scurvy Vitamin A deficiency...vulgaris, *Verucou carcincna *Xoplick spots (neasles) Verzucoas x0nthcmna Epidemid cyst Lyzrlioepithelial cyst Acanthosis nigricans *ai~er I s disease C-4

  9. Androgen Excess Disorders in Women: The Severe Insulin-Resistant Hyperandrogenic Syndrome, HAIR-AN

    Directory of Open Access Journals (Sweden)

    Kristin M. Rager

    2006-01-01

    Full Text Available HAIR-AN syndrome (hyperandrogenism, insulin resistance, acanthosis nigricans is a subset of the polycystic ovary syndrome, where the patients demonstrate severe insulin resistance. It is theorized that both genetic and environmental factors, such as obesity, give rise to the development of HAIR-AN. Diagnosis is primarily clinical, with laboratory values lending further support. Treatment is aimed at decreasing insulin resistance, regulating ovulation, and decreasing acne, acanthosis nigricans, and hirsutism.

  10. Modelling several morphometric relationships of swordfish (Xiphias gladius, black marlin (Makaira indica and blue marlin (Makaira nigricans caught from Indonesian longliners in the eastern Indian Ocean

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    Bram Setyadji

    2016-08-01

    Full Text Available This paper presents several equations for converting among measures of size (length and weight for swordfish (Xiphias gladius, black marlin (Makaira indica and blue marlin (Makaira nigricans caught by Indonesian longliners in the Indian Ocean. The equations use for transforming non-standard measurement i.e. eye fork length (EFL and pectoral fork length (PFL to standard measurement, lower jaw fork length (LJFL. The paper also discussed about the length-weight relationship, including converting from non-standard length (EFL and PFL to round weight. The result showed that both PFL and EFL were positively related to LJFL but there were no significant differences existed between females and males among length measures for swordfish, blue marlin, and black marlin (ANCOVA, P>0.05. All regression equation models were considered to be valid (P<0.01 with coefficient of determinations (r2 ranged from 0.81-0.99. Allometric growth pattern was statistically observed for all swordfish (b=2.94, r2=0.94, black marlin (b=3.12; r2=0.90 and blue marlin (b=3.30; r2=0.91.

  11. Two periods of total testicular regression are peculiar events of the annual reproductive cycle of the black Myotis bat, Myotis nigricans (Chiroptera: Vespertilionidae).

    Science.gov (United States)

    Beguelini, Mateus R; Góes, Rejane M; Taboga, Sebastião R; Morielle-Versute, Eliana

    2014-01-01

    Myotis nigricans presents few and controversial reproductive data, which indicate geographical variation in reproduction. Thus, this study aimed to evaluate the seasonal modifications in testicular and epididymal morphologies in a tropical environment, submitting these organs to morphometric and immunohistochemical analysis. The observations revealed that this species presents two peaks of spermatogenic activity followed by two periods of total testicular regression (a quiescent pre-pubertal-like morphology, where only Sertoli cells and spermatogonia could be observed), in the same annual reproductive cycle, which seem to be only indirectly influenced by abiotic factors. This testicular behaviour seems to be synchronised with the caput and corpus epididymidis, but not with the cauda epididymidis, which presents aspects of sperm storage in May-June. The control of this variation seems to be directly linked to the expression of the androgen receptor, since, throughout the year, it is high in periods of testicular recrudescence and low in periods of deactivation. It is not thought to be directly linked to apoptosis, which is more pronounced in periods of recrudescence than in periods of regression.

  12. Validación del Protocolo de Reproducción de Colossoma macropomum, Piaractus brachypomus y Prochilodus nigricans en Condiciones Controladas

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    Lorgio Verdi-Olivares

    2014-07-01

    Full Text Available Protocolos aplicados a las diferentes etapas de la reproducción  inducida: tratamiento hormonal, incubación, manejo de larvas, y manejo de alevines en los peces de consumo humano Colossoma macropomum (gamitana, Piaractus brachypomus (paco y Prochilodus nigricans (boquichico fueron evaluados en el marco del proyecto UNAP “Cátedra CONCYTEC en Acuicultura Tropical” con el propósito de establecer los adecuados procedimientos en la búsqueda de una óptima productividad acuícola y una mejor rentabilidad económica en Loreto. Con este objetivo se utilizaron como inductores: para gamitana, Pituitaria de Carpa con dosis total de 6,0 mg/kg en hembras y 2,0 mg/kg en machos; Para machos y hembras de paco, Conceptal en dosis total de 2,6 ml/kg en hembras y 1,0 ml/kg en machos; y para boquichico, Pituitaria de Carpa con dosis total de 4,0 mg/kg en hembras y 2,0 mg/kg en machos. El desove se produjo en 11±2 horas; la eclosión ocurre después de 22 horas a partir de la fecundación, con  tasas promedios superiores al 70% y tasas de sobrevivencia, aún cundo por debajo del 50%, garantiza una alta producción de alevines.

  13. Validación del Protocolo de Reproducción de Colossoma macropomum, Piaractus brachypomus y Prochilodus nigricans en Condiciones Controladas

    Directory of Open Access Journals (Sweden)

    Lorgio Verdi Olivares

    2014-06-01

    Full Text Available Protocolos aplicados a las diferentes etapas de la reproducción  inducida: tratamiento hormonal, incubación, manejo de larvas, y manejo de alevines en los peces de consumo humano Colossoma macropomum (gamitana, Piaractus brachypomus (paco y Prochilodus nigricans (boquichico fueron evaluados en el marco del proyecto UNAP “Cátedra CONCYTEC en Acuicultura Tropical” con el propósito de establecer los adecuados procedimientos en la búsqueda de una óptima productividad acuícola y una mejor rentabilidad económica en Loreto. Con este objetivo se utilizaron como inductores: para gamitana, Pituitaria de Carpa con dosis total de 6,0 mg/kg en hembras y 2,0 mg/kg en machos; Para machos y hembras de paco, Conceptal en dosis total de 2,6 ml/kg en hembras y 1,0 ml/kg en machos; y para boquichico, Pituitaria de Carpa con dosis total de 4,0 mg/kg en hembras y 2,0 mg/kg en machos. El desove se produjo en 11±2 horas; la eclosión ocurre después de 22 horas a partir de la fecundación, con tasas promedios superiores al 70% y tasas de sobrevivencia, aún cundo por debajo del 50%, garantiza una alta producción de alevines.

  14. THE ANALYSIS OF CATALASE AND PEROOXIDASE ACTIVITY IN SAPROPHYTIC FUNGUS RHIZOPUS NIGRICANS GROWN ON MEDIUM WHITH DIFERENT CONCENTRATION OF GRINDED CORN CARYOPSIS

    Directory of Open Access Journals (Sweden)

    Tamara Barbaneagra

    2010-09-01

    Full Text Available The purpose of this study was to assay catalase and peroxidase activity in the saprophytic fungus Rhizopus nigricans, grown on mediums containing grinded corn caryopsis, which, in our experiments have replaced carbon source – sucrose in composition of liquid culture medium Czapeck Dox, resulting in the final three experimental variants: V1 = 20 g/l, V2 = 30 g/l, V3 = 40 g/l, while the control variant composition remained unchanged. Measurements were made at three time intervals: 5 days and 10 days and 15 days after inoculation, using fungus mycelium and culture liquid. Determination of catalase activity was performed using Sinha method (Artenie Vl., et al., 2008, and determination of peroxidase was carried out on the basis of ortho-dianisidine method (Cojocaru D.C., 2009. The results showed significant deferens in dynamic of enzyme activity depending on the concentration of carbon source introduced into the medium and age of the fungus.

  15. Efecto de la alometría en la estimación de los parámetros de crecimiento individual del marlín azul Makaira nigricans.

    OpenAIRE

    Jakes Cota, Ulianov

    2013-01-01

    La edad y los parámetros de crecimiento individual (PCI) en peces de pico como el marlin azul Makaira nigricans, usualmente son estimados a partir de cortes transversales de las espinas (CTE) de la aleta dorsal. Para evitar estimaciones sesgadas dada la predominancia de peces grandes en las capturas muestreadas, se deben estimar longitudes a edades previas a su captura (RLs, longitudes retrocalculadas), bajo el supuesto teórico de crecimiento isométrico entre el radio (o diámetro) de los CTE ...

  16. Differential expression of aromatase, estrogen receptor alpha and 17β-HSD associated with the processes of total testicular regression and recrudescence in the bat Myotis nigricans (Chiroptera: Vespertilionidae).

    Science.gov (United States)

    Beguelini, Mateus R; Falleiros, Luiz R; Góes, Rejane M; Rahal, Paula; Morielle-Versute, Eliana; Taboga, Sebastião R

    2014-05-15

    Despite the worldwide distribution and many unique reproductive adaptations that bats present, many aspects of their reproductive hormonal regulation have not been adequately studied, especially in species that presented patterns of total testicular regression. Thus, this study aimed to evaluate the testicular expression of 17β-HSD type 1, aromatase and ERα in the bat Myotis nigricans, during the four periods of its reproductive cycle. Immunoreactivity for ERα was detected only in the cytoplasm of elongated spermatids and in the nuclei of spermatogonia and Sertoli cells. Expression of aromatase was observed in round and elongated spermatids and in Sertoli and Leydig cells. Immunoreactivity for 17β-HSD was restricted to the cytoplasm of Leydig cells. The three expression patterns varied significantly during the four periods of the reproductive cycle. Expression of ERα and aromatase in spermatids was continuous, while expression of ERα in spermatogonia occurred only in initial types (Ap). Expression of ERα and aromatase in Sertoli cells varied, with expression only in periods of spermatogenetic activities; and the same variation was observed for the expression of aromatase and 17β-HSD in Leydig cells. We, therefore, propose that the processes of total testicular regression and posterior recrudescence suffered by M. nigricans from September to January in the northwest of the São Paulo State of Brazil, are directly regulated by testosterone and estrogen. This occurs via the production of testosterone by 17β-HSD, its conversion into estrogen by aromatase, and activation/deactivation of Sertoli cells' AR and spermatogonia's ERα.

  17. Disease: H01228 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H01228 Insulin-resistant diabetes mellitus with acanthosis nigricans (IRAN); Type A... insulin resistance Insulin-resistant diabetes mellitus with acanthosis nigricans (IRAN) is an unusual cause...iagnosis and classification of diabetes mellitus. Diabetes Care 35 Suppl 1:S64-71 (2012) PMID:15232309 (desc...e; Endocrine disease hsa04520(3643) Adherens junction hsa04910(3643) Insulin signaling pathway hsa04930(3643) Type II diabetes mellit...us hsa04960(3643) Aldosterone-regulated sodium reabsorpt

  18. Concentrations of cadmium, Cobalt, Lead, Nickel, and Zinc in Blood and Fillets of Northern Hog Sucker (Hypentelium nigricans) from streams contaminated by lead-Zinc mining: Implications for monitoring

    Science.gov (United States)

    Schmitt, C.J.; Brumbaugh, W.G.; May, T.W.

    2009-01-01

    Lead (Pb) and other metals can accumulate in northern hog sucker (Hypentelium nigricans) and other suckers (Catostomidae), which are harvested in large numbers from Ozark streams by recreational fishers. Suckers are also important in the diets of piscivorous wildlife and fishes. Suckers from streams contaminated by historic Pb-zinc (Zn) mining in southeastern Missouri are presently identified in a consumption advisory because of Pb concentrations. We evaluated blood sampling as a potentially nonlethal alternative to fillet sampling for Pb and other metals in northern hog sucker. Scaled, skin-on, bone-in "fillet" and blood samples were obtained from northern hog suckers (n = 75) collected at nine sites representing a wide range of conditions relative to Pb-Zn mining in southeastern Missouri. All samples were analyzed for cadmium (Cd), cobalt (Co), Pb, nickel (Ni), and Zn. Fillets were also analyzed for calcium as an indicator of the amount of bone, skin, and mucus included in the samples. Pb, Cd, Co, and Ni concentrations were typically higher in blood than in fillets, but Zn concentrations were similar in both sample types. Concentrations of all metals except Zn were typically higher at sites located downstream from active and historic Pb-Zn mines and related facilities than at nonmining sites. Blood concentrations of Pb, Cd, and Co were highly correlated with corresponding fillet concentrations; log-log linear regressions between concentrations in the two sample types explained 94% of the variation for Pb, 73-83% of the variation for Co, and 61% of the variation for Cd. In contrast, relations for Ni and Zn explained <12% of the total variation. Fillet Pb and calcium concentrations were correlated (r = 0.83), but only in the 12 fish from the most contaminated site; concentrations were not significantly correlated across all sites. Conversely, fillet Cd and calcium were correlated across the range of sites (r = 0.78), and the inclusion of calcium in the fillet

  19. Dermatologic manifestation of hyperandrogenism: a retrospective chart review.

    Science.gov (United States)

    Clark, Charlotte M; Rudolph, Jennifer; Gerber, Donald A; Glick, Sharon; Shalita, Alan R; Lowenstein, Eve J

    2014-01-01

    Several studies have described a wide spectrum of hyperandrogenism diseases, many of which are difficult to distinguish from each other. In order to better understand diseases of hyperandrogenism, the authors performed a retrospective study of the cutaneous features and metabolic findings in women with hyperandrogenism. A retrospective chart analysis compiled by three dermatologists in both academic and private settings was performed, including patients presenting with > or = 2 manifestations of hyperandrogenism. Relevant dermatologic and associated manifestations and laboratory and imaging study findings were reviewed. Moderate to severe acne was the most common manifestation. Other common manifestations that patients first presented with include hirsutism, acanthosis nigricans, androgenic alopecia, and skin tags. Oligomenorrhea was the most common systemic presenting sign. Statistical analysis of various clinical markers revealed correlations with hyperandrogenemia. Acanthosis nigricans and hirsutism were found to be useful clinical markers for hyperandrogenism, whereas androgenic alopecia was not. This study provides some insights into the presentation and diverse manifestations seen in hyperandrogenism.

  20. Berardinelli-Seip congenital lipodystrophy in two siblings

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    T Rao

    2014-01-01

    Full Text Available Berardinelli-Seip congenital lipodystrophy (BSCL is a very rare autosomal recessive disorder characterized by various dermatological and systemic manifestations such as lipoatrophy, hypertriglyceridemia, hepatomegaly, acanthosis nigricans, and acromegaloid features. BSCL type 2 is more common and severe, with onset in the neonatal period or in early infancy. The locus for BSCL2 has been identified on chromosome 11q13. Early recognition and differentiation from other congenital generalized lipodystrophies help in the initiation of appropriate preventive and therapeutic measures such as lifestyle modification and pharmacotherapy that helps postpone the onset of metabolic syndrome. We report BSCL type 2 in two siblings with several cutaneous manifestations like acanthosis nigricans, hypertrichosis, prominent subcutaneous veins, and increased lanugo hair.

  1. RABSON-MENDENHALL SYNDROME: A CASE REPORT

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    Mohammad Reza ALAEI

    2010-06-01

    Full Text Available Rabson-Mendenhall syndrome is a rare genetic disorder characterized by growth retardation, dysmorphisms, lack of subcutaneous fat, acanthosis nigricans, enlarged genitalia, hirsutism, dysplastic dentition, coarse facial features, paradoxical fasting hypoglycemia, postprandial hyperglycemia, extreme hyperinsulinemia and pineal hyperplasia. Herein, we described a 10- year-old girl with physical features of the Rabson-Mendenhall syndrome that was presented with polyuria. To our knowledge, this is the first report of the Rabson-Mendenhall syndrome from Iran.

  2. Diabetes mellitus and the skin*

    Science.gov (United States)

    Mendes, Adriana Lucia; Miot, Helio Amante; Haddad Junior, Vidal

    2017-01-01

    Several dermatoses are routinely associated with diabetes mellitus, especially in patients with chronic disease. This relationship can be easily proven in some skin disorders, but it is not so clear in others. Dermatoses such necrobiosis lipoidica, granuloma annulare, acanthosis nigricans and others are discussed in this text, with an emphasis on proven link with the diabetes or not, disease identification and treatment strategy used to control those dermatoses and diabetes. PMID:28225950

  3. [Dowling-Degos disease].

    Science.gov (United States)

    Fernández-Redondo, V; Losada, A; Zulaica, A; Cebreiro, C; Cervantes, R; Toribio, J

    1990-01-01

    We report two cases of Dowling-Degos' disease, one affecting the vulvovaginal semimucose, and one associated to a terminal malignant neoplasia. We remark the importance of this knowledge in order to distinguish it from malignant acanthosis nigricans or other patterns of melanosis or lentiginosis when located within mucose or semimucose, specially at the genitals. All cases associated to malignant neoplasia described are emphasized. However Dowling-Degos' disease is considered to be a nonparaneoplasic genodermatoses, this association should be taken into account.

  4. High sensitivity C - reactive protein (hs-CRP) and clinical characteristics, endocrine, metabolic profile in Indian women with PCOS: a correlation

    OpenAIRE

    Sunita Jaiprakash Ramanand; Jaiprakash B Ramanand; Girish T. Raparti; Ravi R Ghanghas; Nimish R. Halasawadekar; Praveenkumar T. Patil; Mayur P. Pawar; Mayur P. Shinde

    2014-01-01

    Background: Role of hs-CRP was studied in PCOS women. Methods: Correlation between serum hs-CRP and endocrine, metabolic profile was studied in 30 healthy women and 88 PCOS women. In PCOS women correlation between hs-CRP and clinical characteristics viz obesity, infertility, acne, hirsutism, acanthosis nigricans (AN) was also studied. Serum levels of hs-CRP, Luteinizing hormone (LH), Follicle stimulating hormone (FSH), LH:FSH ratio, Testosterone (Testo), fasting insulin, fasting blood gluc...

  5. Type 2 diabetes, polycystic ovary syndrome and the insulin resistance syndrome in adolescents: Are they one big iceberg?

    OpenAIRE

    Dean, Heather J.; Sellers, Elizabeth AC

    2002-01-01

    Obesity in children may cause overt clinical disease in childhood. The complex endocrine and metabolic changes of obesity and insulin resistance in adolescents result in hyperinsulinemia, dyslipidemia, hypertension, steatohepatitis, glucose intolerance, type 2 diabetes, acanthosis nigricans and ovarian hyperandrogenemia, commonly known as polycystic ovarian syndrome (PCOS). Type 2 diabetes and PCOS in adolescents are new endocrine diseases in this age group that require unique approaches to d...

  6. Skin manifestations of acromegaly - a study of 34 cases.

    Science.gov (United States)

    Arya, K R; Krishna, K; Chadda, M

    1997-01-01

    The common dermatological manifestations seen in 34 cases of acromegaly were changes in facial appearance, enlargement of hands and feet, intolerance to heat and sweating, carpal tunnel syndrome, hirsutism, acrochordons and acanthosis nigricans. The mean estimated age of onset was 32.8 years in males and 31.7 years in females, while the mean age at the time of diagnosis was 38.6 years and 36.1 years for males and females respectively, with a slight male preponderance noted.

  7. Biochemical and immunological investigations on hypothyroidism in dogs.

    OpenAIRE

    Gosselin, S J; Capen, C. C.; Martin, S. L.; Targowski, S P

    1980-01-01

    Circulating antibody titers (1:20 to 1:2560) against thyroglobulin were demonstrated in 48% of pet dogs with hypothyroidism by the chromic chloride passive hemagglutination test. Four of six dogs with acanthosis nigricans (1:20) and one of six male dogs with hyperestrogenism (1:40) had low titers of antibody against thyroglobulin whereas clinically normal pet dogs and dogs with other selected endocrinopathies (hypoadrenocorticism, cortisol-excess, diabetes mellitus) or obesity were consistent...

  8. Comparative study of clinical profile of lean and obese polycystic ovary syndrome women

    Directory of Open Access Journals (Sweden)

    Akshaya S.

    2016-08-01

    Conclusions: More than half of women with PCOS were obese. The clinical features like menstrual disturbances, infertility, hirsutism, acne and acanthosis nigricans were present in most of PCOS women irrespective of weight. It is noteworthy that even modest weight loss through diet interventions and increased physical activity has favourable effects on reproductive outcome in PCOS. [Int J Reprod Contracept Obstet Gynecol 2016; 5(8.000: 2530-2533

  9. Cardiometabolic Risk among African-American Women: A Pilot Study

    Science.gov (United States)

    Appel, Susan J.; Oster, Robert A.; Floyd, Natalie A.; Ovalle, Fernando

    2010-01-01

    Objective To determine the associations of the Homeostatic Model of Assessment-insulin resistance (HOMA-ir), acanthosis nigricans, high sensitivity C-reactive protein (hs-CRP), and plasminogen activator inhibitor-1 (PAI-1) with two of the commonly used definitions of the metabolic syndrome (Adult Treatment Panel III {ATP III} and International Diabetes Federation {IDF}) among reproductive age healthy free living African-American women. Methods A pilot study with a cross-sectional design examined 33 African-American women aged 20 to 46 (mean 31.24, +/- 7.25), for the presence of metabolic syndrome determined by ATP III and IDF criteria, insulin resistance (HOMA-ir and/or acanthosis nigricans), degree of inflammation (hs-CRP) and presence of dysfibrinolysis (PAI-1). Results HOMA-ir identified insulin resistance in 27 (81.8%) of the women, whereas the presence of acanthosis nigricans indicated that 16 (48 %) of these women manifested insulin resistance. Metabolic syndrome was found in 7 women (21.2 %) by ATP III or 9 (27.3 %) by IDF criteria. Bivariate correlations showed associations between HOMA-ir and waist circumference, body mass index (BMI), acanthosis nigricans, the ATP III and IDF definitions for metabolic syndrome. PAI-1 was significantly correlated with waist circumference, BMI, fasting glucose, HOMA-ir, and ATP III. Both HOMA-ir and PAI-1 were significantly and negatively correlated with HDL-C. hs-CRP was significantly correlated with BMI and 2-hour post glucose. Conclusion Both dysfibrinolysis (PAI-1 levels) and insulin resistance (HOMA-ir) when individually regressed on the ATP III definition of metabolic syndrome explained 32 % and 29% of the respective variance. The addition of HOMA-ir measurement may significantly improve early recognition of cardiometabolic risk among reproductive age African-American women who have not yet met the criteria for the ATP III or IDF definitions of the metabolic syndrome. Likewise, acanthosis nigricans is potentially a

  10. 变黑白头翁提取物降低亚砷酸盐对小鼠精细胞的毒性%Dihydroxy-isosteviol methyl ester of Pulsatilla nigricans extract reduces arsenic-induced DNA damage in testis cells of male mice: its toxicity, drug-DNA interaction and signaling cascades

    Institute of Scientific and Technical Information of China (English)

    Asmita Samadder; Jayeeta Das; Sreemanti Das; Durba Das; Arnab De; Kakali Bhadra; Anisur Rahman Khuda-Bukhsh

    2012-01-01

    目的:研究变黑白头翁提取物降低亚砷酸盐对小鼠精细胞毒性的作用.方法:给予实验小鼠亚砷酸钠(20 mg/kg每日),并分别在第30、60、90天对小鼠进行检测.亚砷酸盐中毒小鼠被分为2组,其中一组给予变黑白头翁提取物(35 mg/kg),另一组给予85%乙醇.通过检测小鼠精细胞凋亡标志蛋白CYP1A1、p53及caspase 3的活性,确定细胞及DNA的损伤情况,并测定睾丸毒性标志物的水平.通过圆二色光谱仪及熔化温度数据检测变黑白头翁提取物与DNA的相互作用情况.结果:亚砷酸盐中毒小鼠的所有细胞凋亡标志蛋白及睾丸毒性标志物的水平均有所上升,而经变黑白头翁提取物治疗的小鼠上述各项指标均降低或恢复至正常水平.变黑白头翁提取物与DNA相互作用,引起了DNA结构和构象的变化.结论:变黑白头翁提取物可作为砷中毒引起的生殖功能损害的治疗药物给予研究和开发.%OBJECTIVE:To evaluate the ameliorative efficacy of dihydroxy-isosteviol methyl ester (DIME) of Pulsatilla nigricans extract in arsenic-induced DNA damage in testis cells of mice.METHODS:The mice were treated with sodium arsenite (SA) solution intragastrically at a dose of 20 mg/kg per day and examined at 30,60,and 90 dafter treatment.We divided SA-intoxicated mice into two sub-groups:one fed with DIME at a dose of 35 mg/kg and the other with 85% alcohol.We analyzed the expressions of apoptotic signal proteins like CYP1A1,p53 and caspase 3,ascertained the level of cellular and DNA damage and estimated the level of testicular-toxicity biomarkers.We studied the interaction of DIME with calf thymus DNA as target through circular dichroism spectra and melting temperature profiles.RESULTS:We observed an elevation in all apoptotic and toxicity biomarkers leading to cellular and DNA damage in the SA-intoxicated mice which showed significant inhibition or reversal on administration of DIME.Results also showed that

  11. Evaluation of Obesity-Associated Dermatoses in Obese and Overweight Individuals

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    Nurhan Döner

    2011-09-01

    Full Text Available Background and Design: Although the effects of obesity on the skin are known, there are no adequate studies on this subject. To evaluate obesity-related dermatoses in obese and overweight people and to compare the obtained results with those of individuals with body mass index (BMI lower than 25 kg/m2.Material and methods: A total of 500 (mean age: 43.6±12.8; 318 female, 182 male volunteer patients aged 18 years and older, who were treated in inpatient clinics except dermatology, and their relatives were included in the study. The subjects were grouped according to BMI: less than 25 kg/m2 (controls, n = 120, 25-29.9 kg/m2 (overweights, n=130, and greater than 30 kg/m2 (obeses, n=250. The participants were examined dermatologically and all known skin diseases associated with obesity were noted. Results: The total number of dermatoses was statistically higher in obeses and overweights than in controls, as well as in obeses than in overweights. The five most frequently seen dermatoses in all groups were: varicose vein, tinea pedis, striae distensae, acrochordon and onychomycosis, respectively. There were statistically significant differences: between obeses and controls with regard to varicose vein, tinea pedis, striae distensae, acrochordon, onychomycosis, acanthosis nigricans and intertrigo; between overweights and controls with respect to varicose vein, tinea pedis, striae distensae, acrochordon and onychomycosis; between obeses and overweights with regard to striae distensae, acanthosis nigricans and onychomycosis. A positive correlation was detected between BMI and degree of plantar hyperkeratosis in all participants; between BMI and hirsutism score, severity of androgenetic alopecia (AGA and cellulite in women. Conclusion: While acanthosis nigricans and AGA in females were associated with obesity, the other dermatoses were related with not only with obesity but also with increasing weight gain.

  12. Evaluation of Skin Findings in Adult Obese Dermatology Outpatients

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    Hilal

    2011-12-01

    Full Text Available Background: The relationship between systemic diseases and obesity is well-known, but the dermatological effects of obesity are ignored. In the literature, a few articles have appeared concerning the skin findings related to obesity. Our objective was to evaluate the skin findings in obese patients who applied to dermatology outpatient clinic and to compare obese and non-obese outpatients. Material and Method: A total 640 patients (300-in study group, 340-in control group were included in the study. All subjects were asked questions about socio-demographic features and presence of coexisting systemic diseases. Body mass index was calculated, dermatologic examination was performed, and diagnosis was recorded. Data were evaluated statistically. Results: Three-hundred obese patients (study group; 229 females, 71 males, mean age: 45.89 years and 340 non-obese outpatients (control group; 160 females, 180 males, mean age: 38.99 years were included in our study. There were more females in the study group and the mean age was higher than the control group. Most common skin findings of obese patients were: Skin tag (53.3%, plantar hyperkeratosis (29.6%, fungal infections (25.3%, striae (20.3% and acanthosis nigricans (15.3%. Fungal infections, intertrigo, varicose veins and keratosis pilaris were more common in females than males in the study group. Within the study group, patients with skin tag, acanthosis nigricans and plantar hyperkeratosis had higher BMI.Conclusion: Skin tag, plantar hyperkeratosis, fungal infections, striae and acanthosis nigricans were found to be the most common skin diseases in obese patients in our study. Studies related with pathophysiologic features of the skin of obese patients will give more information about the development and treatment of these diseases. (Turk­derm 2011; 45: 184-7

  13. RABSON-MENDENHALL SYNDROME: A CASE REPORT

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    Mohammad Reza ALAEI

    2010-07-01

    Full Text Available Rabson-Mendenhall syndrome is a rare genetic disorder characterized by growth retardation, dysmorphisms, lack of subcutaneous fat, acanthosis nigricans, enlarged genitalia, hirsutism, dysplastic dentition, coarse facial features, paradoxical fasting hypoglycemia, postprandial hyperglycemia, extreme hyperinsulinemia and pineal hyperplasia. Herein, we described a 10- year-old girl with physical features of the Rabson-Mendenhall syndrome that was presented with polyuria. To our knowledge, this is the first report of the Rabson-Mendenhall syndrome from Iran.Keywords:Diabetes, Hirsutism, Insulin resistance, Clitoromegaly

  14. Insulin resistant diabetes mellitus without the presence of insulin antibodies. A case report.

    Science.gov (United States)

    Páv, J; Srámková, J; Matys, Z

    1976-07-01

    A case of a 26-year old woman suffering from an insulin resistant diabetes mellitus for 14 years is described. Acanthosis nigricans was diagnosed in the patient's second year and the syndrome of Stein-Leventhal at the age of 15. Diabetes could not be properly controlled either with the daily dosis of insulin as high as 1140 U or with peroral tolbutamide. Fasting serum IRI concentrations were elevated, the secretoric response to the stimulation by glucose or tolbutamide was substantial and protracted. The hypoglycemic response to the i.v. application of commercial insulin was insignificant. Serum growth hormone levels were normal. No presence of insulin antibodies in the serum was detected.

  15. Skin manifestations of acromegaly - a study of 34 cases

    Directory of Open Access Journals (Sweden)

    Arya Kavita

    1997-01-01

    Full Text Available The common dermatological manifestations seen in 34 cases of acromegaly were changes in facial appearance, enlargement of hands and feet, intolerance to heat and sweating, carpal tunnel syndrome, hirsutism, acrochordons and acanthosis nigricans. The mean estimated age of onset was 32.8 years in males and 31.7 years in females, while the mean age at the time of diagnosis was 38.6 years and 36.1 years for males and females respectively, with a slight male preponderance noted.

  16. Berardinelli-Seip syndrome in a 6-year-old boy

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    Babu Priya

    2008-01-01

    Full Text Available A 6-year-old boy presented with abnormal habitus since birth, delayed language development, history of frequent falls since 9 months, and fever since 1 week. He was found to have hyperandrogenic features, generalized paucity of fat, generalized muscular overdevelopment, and brownish pigmentation over the flexural creases. Skin biopsy demonstrated features suggestive of acanthosis nigricans with an absence of subcutaneous tissue. After further investigation, a diagnosis of Berardinelli-Seip syndrome with bilateral pneumonia and generalized tonic clonic seizures was made. Clinical features, histopathology, differential diagnosis, and prognosis of this rare disorder have been discussed.

  17. POLYCYSTIC OVARIAN DISEASE: A DERMATOLOGIST’S VIEWPOINT

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    Hassan Iffat

    2011-04-01

    Full Text Available Polycystic ovary syndrome(PCOS is a common condition characterized by menstrual abnormalities and clinical or biochemical features of hyperandrogenism. Features of PCOS may manifest at any age, ranging from childhood (premature puberty, teenage years (hirsutism, menstrual irregularities, early adulthood and middle life (infertility, glucose intolerance to later life (diabetes mellitus and cardiovascular disease. Cutaneous manifestations of PCOS are protean and include hirsutism, acne, androgenic alopecia and acanthosis nigricans. Treatment of cutaneous manifestations of PCOS requires a coordinated team approach. A combination of drug therapy, counseling and cosmetic procedures can maximize the results.

  18. Polycystic ovary syndrome: a review for dermatologists: Part I. Diagnosis and manifestations.

    Science.gov (United States)

    Housman, Elizabeth; Reynolds, Rachel V

    2014-11-01

    Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women who are of reproductive age. The pathogenesis involves several associated hormonal pathways that culminate in metabolic, reproductive, and cardiovascular effects. The hallmark features of hyperandrogenism and hyperinsulinemia have systemic long-term implications. Dermatologists frequently evaluate and manage the cutaneous manifestations of PCOS (ie, acanthosis nigricans, hirsutism, acne, and alopecia), and therefore play a key role in its diagnosis and management. In part I of this continuing medical education article, we review the definition, etiology, pathogenesis, and clinical features of PCOS.

  19. Autoimmune Hypoglycemia in a Patient with Characterization of Insulin Receptor Autoantibodies

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    Suk Chon

    2011-02-01

    Full Text Available BackgroundType B insulin resistance syndrome is a manifestation of autoantibodies to the insulin receptor that results in severe hyperglycemia and acanthosis nigricans. However, the mechanisms by which these autoantibodies induce hypoglycemia are largely unknown. In this paper, we report the case of patient with type B insulin resistance syndrome who presented with frequent severe fasting hypoglycemia and acanthosis nigricans.MethodsTo evaluate the mechanism of hypoglycemia, we measured the inhibition of insulin binding to erythrocytes and IM9 lymphocytes in a sample of the patient's dialyzed serum before and after immunosuppressive therapy.ResultsIn the patient's pre-treatment serum IgG, the binding of 125I-insulin to erythrocytes was markedly inhibited in a dose-dependent manner until the cold insulin level reached 10-9 mol/L. We also observed dose-dependent inhibition of insulin binding to IM9 lymphocytes, which reached approximately 82% inhibition and persisted even when diluted 1:20. After treatment with glucocorticoids, insulin-erythrocyte binding activity returned to between 70% and 80% of normal, while the inhibition of insulin-lymphocyte binding was reduced by 17%.ConclusionWe treated a patient with type B insulin resistance syndrome showing recurrent fasting hypoglycemia with steroids and azathioprine. We characterized the patient's insulin receptor antibodies by measuring the inhibition of insulin binding.

  20. Correlation of skin changes with hormonal changes in polycystic ovarian syndrome: A cross-sectional study clinical study

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    B Vijaya Gowri

    2015-01-01

    Full Text Available Background: Polycystic ovarian syndrome (PCOS is a heterogenous collection of signs and symptoms that when gathered, form a spectrum of disorder with disturbance of reproductive, endocrine and metabolic functions. Aim: The aim of this study is to correlate the skin manifestations with hormonal changes and to know the incidence and prevalence of skin manifestations in patients with PCOS. Materials and Methods: A total of 40 patients with PCOS were examined during 1 year time period from May 2008 P to May 2009. Detailed clinical history was taken from each patient. PCOS was diagnosed on the basis of ultrasonography. Hormonal assays included fasting blood sugar, postprandial blood sugar, follicle-stimulating hormone, luteinizing hormone, thyroid stimulating hormone, dehydroepiandrostenedione, prolactin, free testosterone, fasting lipid profile and sex hormone binding globulin. The results obtained were statistically correlated. Results: In our study, the prevalence of cutaneous manifestations was 90%. Of all the cutaneous manifestations acne was seen in highest percentage (67.5%, followed by hirsutism (62.5%, seborrhea (52.5%, androgenetic alopecia (AGA (30%, acanthosis nigricans (22.5% and acrochordons (10%. Fasting insulin levels was the most common hormonal abnormality seen in both acne and hirsutism, whereas AGA was associated with high testosterone levels. Conclusion: The prevalence of cutaneous manifestations in PCOS was 90%. Hirsutism, acne, seborrhea, acanthosis nigricans and acrochordons were associated with increased levels of fasting insulin, whereas AGA showed higher levels of serum testosterone.

  1. Coexistencia de acromegaloidismo y cáncer de pulmón Coexistence of acromegaloidism and lung carcinoma

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    Graciela Stalldecker

    2010-06-01

    Full Text Available El acromegaloidismo es una entidad en la cual se encuentran presentes algunos rasgos acromegálicos sin evidencia bioquímica de hipersecreción de hormona de crecimiento (GH, siendo la resistencia insulínica una de las posibles etiologías. Se presenta un paciente con rasgos acromegálicos, proceso neoplásico en pulmón izquierdo, acantosis nigricans (AN generalizada y marcada resistencia a la insulina (IR. Se descartó acromegalia ante la presencia de una IGF1 normal y GH Acromegaloidism is a condition characterized by features of acromegaly without biochemical evidence of excessive growth hormone (GH production. Insulin resistance is one of the possible etiologies. We report the case of a patient with acromegalic features, a left lung neoplastic process, generalized acanthosis nigricans and severe insulin resistance. Normal IGF 1 and GH inhibition below 1 ng/ml by the oral glucose tolerance test ruled out the diagnosis of acromegaly. Acanthosis nigricans (AN may be present as a paraneoplastic syndrome or may be associated to benign pathology in which insulin resistance is involved. Acromegaloidism has been reported in patients with severe insulin resistance as we are describing here. It is possible that very high insulin levels can stimulate excessive acral growth acting through insulin receptors that retain the ability to mediate anabolic effects, or alternatively through the IGF1 receptor. We found no data of acromegaloidism as a paraneoplastic syndrome. To summarize, we report a case of severe insulin resistance, pseudoacromegaly and AN, coexisting with a lung carcinoma. Acromegaloidism and AN appear to be due to insulin resistance, even though in this patient we cannot exclude that AN could be a paraneoplastic syndrome.

  2. Cellular polymorphism and nitrogenase activity of Azotobacter nigricans During Kerosene removal

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    Garcia-Esquivel, G.; Rodriguez-Casasola, T.; Ferrera-Cerrato, R.; Rodriguez-Vazquez, R.; Calva-Calva, G.; Esparza-Garcia, F.

    2009-07-01

    It has been reported that certain plants grow in soils contaminated with hydrocarbons developing nitrogen-fixing bacteria. for example in the rhizosphere of beams plants grown in soil contaminated with kerosene it was found the presence of Azotobacter which removed kerosene and produced polymorphic cells when they grew in nitrogen free cultures. (Author)

  3. Effects of climatic factors on height growth components in Austrian black pine. [Pinus nigra nigricans, Arn

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    Guyon, J.P.

    1986-01-01

    Weekly (or biweekly) leader shoot growth was assessed over 3 growing seasons (1982-1984) and annual shoot length was measured over 6 years (1979-1984) for a 30 seedlings (age: 11 years) sampled in a naturally regenerated stand of black pine, located at Mont Ventoux in southern France. The number of internodes (stem units) was assessed over the same period (1979-1984). Annual shoot length of a given year appeared as mainly controlled by the number of internodes initiated during the summer of the previous year. Investigations of climatic factors taking place during the assumed initiation stage, have shown a significant correlation between the annual leader shoot length and the cummulated rainfall of June, July and September of the previous year. By contrast, the correlations between the temperature factors during the assumed time of initiation and number of initiated internodes of the same year were not significant. The weekly shoot growth was significantly related to the average of minimum air temperature of the same period, at the time of height growth, that is to say April and May. Finally the possibility of integrating these results into growth models is discussed.

  4. A study on the cutaneous manifestations of diabetes mellitus

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    Neerja Puri

    2012-04-01

    Full Text Available The cutaneous manifestations of diabetes mellitus are varied. We conducted a study of fifty patients having diabetes mellitus coming from the department of dermatology and medicine. The commonest cutaneous feature of diabetes were pyodermas seen in 40% patients, dermatophytosis seen in 36% patients, pruritis diabetic thick skin seen in 20 % patients, diabetic dermopathy seen in 16% patients, diabetic bulla and rubeosis seen in 8% patients each and meralgia paraesthetica and diabetic foot seen in 4% patients each. About the associations of diabetes mellitus, achrchordons were seen in 8% patients, vitiligo and perforating dermatoses were seen in 6% patients each, granuloma annulare, eruptive xanthomas, acanthosis nigricans, necrobiosis lipoidica and oral lichen planus were seen in 4 % patients each and xanthelasma was seen in 2% patients.

  5. Nonalcoholic Fatty Liver Disease in Pediatrics.

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    Duncan, Martin; Zong, Wenjing; Biank, Vincent F; Hageman, Joseph R

    2016-02-01

    A 16-year-old Hispanic girl with an elevated body mass index in an otherwise normal state of health presented for her well-child examination. She had signs of metabolic syndrome and insulin resistance including increased waist circumference and acanthosis nigricans. Laboratory results revealed elevated transaminases with otherwise normal hepatic function. Based on the physical examination and laboratory results, she was diagnosed with nonalcoholic fatty liver disease (NAFLD). After further evaluation, she eventually underwent a liver biopsy. The biopsy revealed nonalcoholic steatohepatitis (NASH) with stage 2 fibrosis. This article reviews the definition of NAFLD and NASH, an increasingly prevalent cause of pediatric chronic liver disease associated with obesity and metabolic syndrome. The article also outlines the epidemiology, risk factors, and natural history of NAFLD, which may help identify and prevent high-risk pediatric patients from progressing to irreversible liver disease. Understanding the diagnostic and treatment options offers the best chance at preventing and reversing the early stages of this disease.

  6. Metformin - For the dermatologist

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    Aditya Kumar Bubna

    2016-01-01

    Full Text Available Metformin though primarily an antidiabetic drug, has found to play an important role in a number of cutaneous disorders. Because of its role in improving hyperinsulinemia, it has proven beneficial in hormonal acne, hidradenitis suppurativa (HS and acanthosis nigricans. Its antiandrogenic properties further serve as an add-on to the conventional management of hirsutism associated with polycystic ovarian syndrome. Very recently, systemic usage of metformin for psoriasis and cutaneous malignancies has shown promising results. Interestingly, metformin has also been topically used in hyperpigmentary disorders with pertinent levels of improvement and happens to be the most recent addition to the list of dermatologic indications. Though an oral hypoglycemic agent to begin with, metformin today has proven to be a boon for dermatologists.

  7. Metformin - For the dermatologist.

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    Bubna, Aditya Kumar

    2016-01-01

    Metformin though primarily an antidiabetic drug, has found to play an important role in a number of cutaneous disorders. Because of its role in improving hyperinsulinemia, it has proven beneficial in hormonal acne, hidradenitis suppurativa (HS) and acanthosis nigricans. Its antiandrogenic properties further serve as an add-on to the conventional management of hirsutism associated with polycystic ovarian syndrome. Very recently, systemic usage of metformin for psoriasis and cutaneous malignancies has shown promising results. Interestingly, metformin has also been topically used in hyperpigmentary disorders with pertinent levels of improvement and happens to be the most recent addition to the list of dermatologic indications. Though an oral hypoglycemic agent to begin with, metformin today has proven to be a boon for dermatologists.

  8. Treatment of Abnormal Glucose Regulation and Huge Ovarian Cysts with High Dose Insulin Glargine in an Infant with Leprechaunism - Case Report

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    Ayşe Yasemin Çelik

    2010-12-01

    Full Text Available Introduction: Leprechaunism is a rare autosomal recessive disorder caused by mutations in the insulin receptor gene. In this report; we present a 75 days old infant with leprecahunism treated by high dose insulin glargine.Case Report: Yetmiş day old girl was diagnosed as leprechaunism because of the hyperglycemia, ketoacidosis and dysmorphic appearance. Huge cysts with multiple septa were determined in her ovaries. High dose insulin glargine were adjusted to achieve target blood glucose regulation. Huge ovarian cysts resolved by this treatment.Conclusion: Leprechaunism is characterized by intra-uterine and postnatal growth restriction, lipo-atrophy, characteristic facial features, severe acanthosis nigricans, abnormal glucose homeostasis, clitoromegaly and hirsutism. It is usually fatal within the 1st year of life because of diabetic ketoacidosis or recurrent infections. (Journal of Current Pediatrics 2010; 8: 119-22

  9. Metabolic syndrome and skin: Psoriasis and beyond

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    Tanmay Padhi

    2013-01-01

    Full Text Available Metabolic syndrome (Met S is a clustering of risk factors comprising of abdominal obesity, dyslipidemia, elevated blood pressure, and abnormal glucose tolerance. The prevalence of Met S has been increasing in the last few years throughout the world. Psoriasis has consistently been associated with Met S as well as its various components. However, the association is no longer limited to psoriasis alone. Various dermatological conditions such as lichen planus, androgenetic alopecia, systemic lupus erythematosus, skin tags, acanthosis nigricans, and even cutaneous malignancies have also been found to be associated with this syndrome. Though chronic inflammation is thought to be the bridging link, the role of oxidative stress and endocrine abnormalities has recently been proposed in bringing them together.

  10. Berardinelli–Seip syndrome type 2 – An Egyptian child

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    Rabah M. Shawky

    2015-04-01

    Full Text Available We report a 2.5 year old male, first in order of birth of first cousin consanguineous parents with the typical features of Berardinelli–Seip congenital lipodystrophy 2 (BSCL2 since birth with moderate mental retardation. He had generalized lipodystrophy with various dermatologic and systemic manifestations. The patient looked older than his age with the loss of buccal pad of fat, hypertrichosis mainly on the back and lower limbs, thick scalp hair, mild prognathism, large hands and feet with prominent joints and muscular hypertrophy. Acanthosis nigricans was evident over the neck and both axillae inspite of the normal level of sugar and insulin. The abdomen was markedly prominent with mild hepatosplenomegaly and enlarged external genitals. Echo-cardiography demonstrated cardiac hypertrophy. Triglyceride level was high with reduced high density lipoproteins (HDL.

  11. Acne-associated syndromes: models for better understanding of acne pathogenesis.

    Science.gov (United States)

    Chen, W; Obermayer-Pietsch, B; Hong, J-B; Melnik, B C; Yamasaki, O; Dessinioti, C; Ju, Q; Liakou, A I; Al-Khuzaei, S; Katsambas, A; Ring, J; Zouboulis, C C

    2011-06-01

    Acne, one of the most common skin disorders, is also a cardinal component of many systemic diseases or syndromes. Their association illustrates the nature of these diseases and is indicative of the pathogenesis of acne. Congenital adrenal hyperplasia (CAH) and seborrhoea-acne-hirsutism-androgenetic alopecia (SAHA) syndrome highlight the role of androgen steroids, while polycystic ovary (PCO) and hyperandrogenism-insulin resistance-acanthosis nigricans (HAIR-AN) syndromes indicate insulin resistance in acne. Apert syndrome with increased fibroblast growth factor receptor 2 (FGFR2) signalling results in follicular hyperkeratinization and sebaceous gland hypertrophy in acne. Synovitis-acne-pustulosis-hyperostosis-osteitis (SAPHO) and pyogenic arthritis-pyoderma gangrenosum-acne (PAPA) syndromes highlight the attributes of inflammation to acne formation. Advances in the understanding of the manifestation and molecular mechanisms of these syndromes will help to clarify acne pathogenesis and develop novel therapeutic modalities.

  12. Polycystic ovarian syndrome

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    Nina Madnani

    2013-01-01

    Full Text Available Polycystic ovarian syndrome (PCOS is a "multispeciality" disorder suspected in patients with irregular menses and clinical signs of hyperandrogenism such as acne, seborrhoea, hirsutism, irregular menses, infertility, and alopecia. Recently, PCOS has been associated with the metabolic syndrome. Patients may develop obesity, insulin resistance, acanthosis nigricans, Type 2 diabetes, dyslipidemias, hypertension, non-alcoholic liver disease, and obstructive sleep apnoea. Good clinical examination with hematological and radiological investigations is required for clinical evaluation. Management is a combined effort involving a dermatologist, endocrinologist, gynecologist, and nutritionist. Morbidity in addition includes a low "self image" and poor quality of life. Long term medications and lifestyle changes are essential for a successful outcome. This article focuses on understanding the normal and abnormal endocrine functions involved in the pathogenesis of PCOS. Proper diagnosis and management of the patient is discussed.

  13. Feeding of blue marlin Makaira nigricans off Mazatlan, Sinaloa, Mexico Alimentación del marlin azul Makaira nigricans del área de Mazatlán, Sinaloa, México

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    Leonardo Abitia-Cárdenas

    2010-01-01

    Full Text Available We analyzed the stomach contents of 52 blue marlins caught between October 2002 and October 2004 by the sport-fishing fleet of Mazatlan, Sinaloa, in the gulf of California, Mexico. Blue marlin feed on 15 food items. According to the index of relative importance (IRI, the most important prey were the frigate or bullet mackerel Auxis spp. (52% and jumbo squid Dosidicus gigas (30%.Se analiza el contenido estomacal de 52 marlines azules capturados por la flota de pesca deportiva que operó en el área de Mazatlan, Sinaloa, en el golfo de California, México, durante el periodo octubre 2002 a octubre 2004. El marlin azul se alimenta de 15 categorías alimenticias (ítemes, de las cuales las más importantes de acuerdo al índice de importancia relativa (IRI, fueron el pez melva Auxis spp. (52% y el calamar gigante Dosidicus gigas (30%.

  14. Variation in body mass dynamics among sites in Black Brant Branta bernicla nigricans supports adaptivity of mass loss during moult

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    Fondell, Thomas F.; Flint, Paul L.; Schmutz, Joel A.; Schamber, Jason L.; Nicolai, Christopher A.

    2013-01-01

    Birds employ varying strategies to accommodate the energetic demands of moult, one important example being changes in body mass. To understand better their physiological and ecological significance, we tested three hypotheses concerning body mass dynamics during moult. We studied Black Brant in 2006 and 2007 moulting at three sites in Alaska which varied in food availability, breeding status and whether geese undertook a moult migration. First we predicted that if mass loss during moult were simply the result of inadequate food resources then mass loss would be highest where food was least available. Secondly, we predicted that if mass loss during moult were adaptive, allowing birds to reduce activity during moult, then birds would gain mass prior to moult where feeding conditions allowed and mass loss would be positively related to mass at moult initiation. Thirdly, we predicted that if mass loss during moult were adaptive, allowing birds to regain flight sooner, then across sites and groups, mass at the end of the flightless period would converge on a theoretical optimum, i.e. the mass that permits the earliest possible return to flight. Mass loss was greatest where food was most available and thus our results did not support the prediction that mass loss resulted from inadequate food availability. Mass at moult initiation was positively related to both food availability and mass loss. In addition, among sites and years, variation in mass was high at moult initiation but greatly reduced at the end of the flightless period, appearing to converge. Thus, our results supported multiple predictions that mass loss during moult was adaptive and that the optimal moulting strategy was to gain mass prior to the flightless period, then through behavioural modifications use these body reserves to reduce activity and in so doing also reduce wing loading. Geese that undertook a moult migration initiated moult at the highest mass, indicating that they were more than able to compensate for the energetic cost of the migration. Because Brant frequently change moult sites between years in relation to breeding success, the site-specific variation in body mass dynamics we observed suggests individual plasticity in moult body mass dynamics.

  15. [IMPACT OF SIX COOKING TECHNIQUES ON FATTY ACID COMPOSITION OF MARLIN (MAKAIRA NIGRICANS) AND HAKE (MERLUCCIUS PRODUCTUS)].

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    Castro-González, María Isabel; Carrillo-Domínguez, Silvia

    2015-09-01

    Introducción: el consumo frecuente de pescado aporta beneficios a la salud por su contenido en ácidos grasos n-3. Generalmente se consume cocinado pero la cocción mejora o perjudica la concentración de ácidos grasos(AG), pues son susceptibles a oxidarse por las temperaturas y tiempos de cocción. Objetivos: analizar el efecto de seis técnicas de cocción sobre los lípidos totales (LT) y AG de marlín y merluza y seleccionar la que favorece una mejor conservación de AG de importancia para la salud. Métodos: se sometieron los filetes a diferentes técnicas de cocción: vapor (VA), empapelado papel-aluminio (EA), empapelado hoja-plátano (EP), horno-gas (HG), horno-microondas (HM) y sofrito (SF). Los AG se identificaron por cromatografía de gases/FID. Resultados y discusión: Marlín: el SF aumentó la concentración de LT y el HM la disminuyó. Estadísticamente, los AGS, AGP y EPA + DHA aumentaron con EA, los AGM disminuyeron con EP y aumentaron en el SF. Merluza: el SF aumentó la concentración en todos los grupos de AG, mientras que el VA la disminuyó. Por los valores de AGS, índices de aterogenicidad (IA), trombogenicidad (IT), susceptibilidad a la peroxidación y relación hipocolesterolémica/ hipercolesterolémica de los ácidos grasos (HH) encontrados, se sugiere que la merluza es un pescado con mayores beneficios para la salud, independientemente de la técnica que se aplique. Por su contenido en EPA + DHA el marlín parece ser una excelente opción si se cocina en papel aluminio. La técnica de sofrito en ambas especies disminuyó el IA, IT y aumentó significativamente la HH, sin embargo la relación n3/n6 disminuyó. La técnica menos conveniente para ambas especies resultó ser el VA.

  16. 肥胖儿童尿酸水平与心血管代谢异常及非酒精性脂肪肝的关系%The association between uric acid levels and cardiovascular metabolic disorders and non - alcohol fatty liver disease among obese children

    Institute of Scientific and Technical Information of China (English)

    闫银坤; 米杰; 侯冬青; 段佳丽; 孙颖; 程红; 赵小元; 黄贵民; 丁文清; 刘琴

    2015-01-01

    Objective To observe the prevalence of hyperuricemia(HUA)among obese children,and to ex-plore the association between uric acid(UA)levels and cardiometabolic risk factors,acanthosis nigricans and non - al-cohol fatty liver disease(NAFLD). Methods By using representative sampling method,1 753 obese children aged 6 -17 years old from 18 schools in 3 districts of Beijing(Xicheng,Haidian,Miyun)were selected to participate in the clini-cal examinations,including anthropometric measurements(height,weight)and blood pressure. Serum biochemical pa-rameters were assessed,including fasting plasma glucose(FPG),total cholesterol(TC),triglyceride(TG),high - densi-ty lipoprotein cholesterol(HDL - C),low - density lipoprotein cholesterol(LDL - C)and UA. Acanthosis nigricans and B - model ultrasonography of the liver were conducted. Results The prevalence of hypertension,impaired fasting glu-cose,dyslipidemia,acanthosis nigricans,and NAFLD among these 1 753 obese children was 33. 6%(589 cases), 66. 5%(1 156 cases),54. 3%(943 cases),23. 3%(408 cases),and 17. 0%(298 cases),respectively. The preva-lence of HUA was 40. 70%(714 / 1 753 cases),with 50. 17%(581 / 1 158 cases)in boys and 22. 34%(133 / 595 ca-ses)in girls. There was a significant increase in body mass index,systolic blood pressure,diastolic blood pressure, FPG,TG and LDL - C with the increase of UA,but there was a decrease in HLD - C with the increase of UA(all P ﹤0. 05). In boys,the adjusted odds ratios( OR)and 95% CI of the highest quartile of UA for hypertension,impaired fasting glucose,dyslipidemia,acanthosis nigricans,and NAFLD were 1. 16(0. 77 - 1. 74),1. 34(0. 90 - 1. 99),1. 29 (0. 89 - 1. 87),1. 89(1. 17 - 3. 04),and 1. 71(1. 03 - 2. 84),respectively;in girls,the adjusted OR and 95% CI of the highest quartile of UA for hypertension,impaired fas-ting glucose,dyslipidemia,acanthosis nigricans,and NAFLD was 0. 70(0. 40 - 1. 24),0. 60(0. 40 - 1. 00),1. 69(1. 04 - 2. 70),1. 67(0. 80 - 3. 49),and 1. 33(0. 48 - 3. 66),re

  17. As principais alterações dermatológicas em pacientes obesos Major dermatological changes in obese patients

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    Rosimeri da Silva Castanho Mendonça

    2011-03-01

    Full Text Available INTRODUÇÃO: A obesidade é considerada grave doença crônica que vem atingindo proporções mundiais gerando transtornos sérios para a saúde pública. No Brasil, a prevalência desta doença denota aumento progressivo em relação ao número de pessoas com sobrepeso e/ou obesidade. Apesar disso, as manifestações dermatológicas da obesidade têm sido pouco estudadas. OBJETIVO: Abordar as dermatoses que mais acometem pacientes obesos e seu tratamento, principalmente na aplicação adequada da fisioterapia dermatofuncional. MÉTODO: Revisão da literatura, entre 2000 e 2010, de artigos indexados nas bases Medline/Pubmed, Scielo e Lilacs, livros e monografias das bibliotecas da Universidade de Fortaleza e Universidade Federal do Ceará, Fortaleza, CE, Brasil, cruzando os descritores obesidade, dermatose e fisioterapia. Foram reconhecidas como mais importantes as seguintes situações patológicas: estrias, celulite, acanthosis nigricans, acrocórdons, flacidez, úlcera e impetigo. Entre as formas de tratamento deuse destaque à atuação da fisioterapia dermatofuncional. CONCLUSÃO: O tratamento das dermatoses por meio da fisioterapia dermatofuncional é possível podendo trazer consigo resultados que satisfaçam a expectativa dos pacientes e proporcionando-lhes melhor qualidade de vida.INTRODUCTION: Obesity is a serious chronic disease that has reached global proportions causing problems to public health. In Brazil, the prevalence of this disease shows a progressive increase in the number of people with overweight or obesity. Nevertheless, the skin manifestations have not been well studied. OBJECTIVE: To analyse the dermatosis most affecting obese patients and its treatment, particularly in proper application of dermatological therapy. METHOD: Was made a literature review between 2000 and 2010 of articles published in Medline/ PubMed, SciELO and Lilacs, books and monographs of the libraries of the University of Fortaleza and the Federal

  18. Prevalence of insulin resistance and its association with metabolic syndrome criteria among Bolivian children and adolescents with obesity

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    Rodriguez Susana

    2008-08-01

    Full Text Available Abstract Background Obesity is a one of the most common nutritional disorder worldwide, clearly associated with the metabolic syndrome, condition with implications for the development of many chronic diseases. In the poorest countries of Latin America, malnourishment is still the most prevalent nutritional problem, but obesity is emerging in alarming rates over the last 10 years without a predictable association with metabolic syndrome. The objective of our study was to determine the association between insulin-resistance and components of the metabolic syndrome in a group of Bolivian obese children and adolescents. The second objective was determining the relation of acanthosis nigricans and insulin-resistance. Methods We studied 61 obese children and adolescents aged between 5 and 18 years old. All children underwent an oral glucose tolerance test and fasting blood sample was also obtained to measure insulin, HDL, LDL and triglycerides serum level. The diagnosis of metabolic syndrome was defined according to National Cholesterol Education Program-Adult Treatment Panel (NCEP-ATP III criteria adapted for children. Results Metabolic syndrome was found in 36% of the children, with a higher rate among males (40% than females (32.2% (p = 0.599. The prevalence of each of the components was 8.2% in impaired glucose tolerance, 42.6% for high triglyceride level, 55.7% for low levels of high-density lipoprotein cholesterol, and 24.5% for high blood pressure. Insulin resistance (HOMA-IR > 3.5 was found in 39.4% of the children, with a higher rate in males (50% than females (29%. A strong correlation was found between insulin resistance and high blood pressure (p = 0.0148 and high triglycerides (p = 0.002. No statistical significance was found between the presence of acanthosis nigricans and insulin resistance. Conclusion Metabolic syndrome has a prevalence of 36% in children and adolescent population in the study. Insulin resistance was very common among

  19. Clinical features and detection of some hormones in the patients with polycystic ovary syndrome%多囊卵巢综合征临床特征和某些内分泌激素的检测

    Institute of Scientific and Technical Information of China (English)

    冯金鸽; 朱威; 申艳; 孟焱

    2012-01-01

    Objective: To compare the differences in dermatologic and metabolic features in polycystic ovary syndrome (PCOS) between the patients with obesity and those without obesity. Methods: A total of 116 cases of PCOS were divided into obese and non- obese groups according to the body mass index. Each of women underwent physical assessment including acne, hirsutism, seborrhea, androgenic alopecia and acanthosis nigricans. Correlation between the dermatologic features and metabolic parameters were analyzed with multivariate logistic regression models. Results: The prevalence rates of acne, hirsutism, seborrhea, androgenic alopecia and acanthosis nigricans were 5.2% vs40.5%, 29.3% vs37.0%, 14.7% vs22.4%, 14.7% vs24.1 and 1.7% vs4.3% in obesity and non - obesity groups, respectively. PCOS women with obesity had a higher risk of developing oligomenorrhea, a longer menses and a higher testosterone level. Conclusions: PCOS patients with obesity have more dermatologic disorders and ovulation dysfunction.%目的:比较肥胖和非肥胖性多囊卵巢综合征(PCOS)患者的皮肤病学特征和性激素水平的差异.方法:依据BMI标准将患者分为肥胖组(41例)和非肥胖组(75例),对各组中每个妇女进行体质评估,包括体型、痤疮、多毛症、皮脂溢出、雄激素性脱发和黑棘皮病.logistic多元回归分析皮肤病学特征与激素和代谢参数的相关性.结果: 肥胖和非肥胖组痤疮的发生率分别为5.2%和40.5%,多毛症为29.3%和37.0%,皮脂溢出为14.7%和22.4%,雄激素性脱发为14.7%和24.1%,黑棘皮病为1.7%和4.3%.与非肥胖组比较,肥胖组PCOS月经减少、经期延长,睾酮水平明显增高.结论:肥胖PCOS患者会出现更多的皮肤症状和排卵障碍.

  20. 补肾活血祛痰方治疗肥胖型与非肥胖型多囊卵巢综合征的疗效研究%Study on Clinical Effects of Kidney-invigorating Blood-activating Phlegm-eliminating Prescription in Treating Polycystic Ovary Syndrome of Obesity Type and Non-obesity Type

    Institute of Scientific and Technical Information of China (English)

    王针织; 周丽虹; 俞超芹; 韩洁; 翟东霞

    2014-01-01

    目的:观察补肾活血祛痰方治疗肥胖型与非肥胖型多囊卵巢综合征(Polycystic ovary syn-drome,PCOS)的临床疗效。方法:依据 PCOS标准,收集肥胖型PCOS患者与非肥胖型PCOS患者共52例,采用补肾活血祛痰方进行治疗。观察患者治疗前后月经周期、排卵、肥胖、多毛、黑棘皮症、痤疮等临床症状及血睾酮(Testosterone,T)、胰岛素(Insulin,INS)、黄体生成激素(Luteoinizing hormone,LH)、卵泡刺激素(Fol-licle stimulating hormone,FSH)等变化情况。结果:补肾活血祛痰方既能改善患者肥胖、多毛、黑棘皮症、痤疮等临床症状(P<0.05),又能降低患者的血T、INS和LH水平(P<0.05)。结论:补肾活血祛痰方可降低PCOS患者的血T水平,改善患者胰岛素抵抗状态,对PCOS有较好的治疗效果。%Objective:To observe clinical effects of kidney-invigorating blood-activating phlegm-eliminating prescription in treating polycystic ovary syndrome (PCOS) of obesity type and non-obesity type. Methods:According to the standard of PCOS, 52 cases were collected and treated with kidney-invigorating blood-activating phlegm-eliminating prescription. Changes of testosterone (T), insulin (INS), luteinizing hormone (LH), follicle stimulating hormone (FSH) and clinical symptoms such as menstrual cycle, ovulation, obesity, hairiness, acanthosis nigricans, acne and others of both groups were observed before and after treating. Results: Kidney-invigorating blood-activating phlegm-eliminating prescription could not only improve clinical symptoms including obesity, hairiness, acanthosis nigricans and acnes of the patients, but also decrease the levels of blood T, INS and LH (P<0.05). Conclusion:Kidney-invigorating blood-activating phlegm-eliminating prescription could decrease the level of blood T, improve insulin resistance of the patients and it is effective in treating PCOS.

  1. Markers of insulin resistance and sedentary lifestyle are predictors of preeclampsia in women with adverse obstetric results.

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    Hoirisch-Clapauch, S; Benchimol-Barbosa, P R

    2011-12-01

    Some thrombophilias and severe preeclampsia may increase the risk for preterm deliveries and fetal death due to placental insufficiency. Our objective was to evaluate clinical and laboratory data as predictors of preeclampsia in a population of mothers with 3rd trimester fetal losses or preterm deliveries. In a longitudinal retrospective study, 54 consecutive women (age range: 16 to 39 years) with normotensive pregnancies were compared to 79 consecutive women with preeclampsia (age range: 16 to 43 years). Weight accrual rate (WAR) was arbitrarily defined as weight gain from age 18 years to the beginning of pregnancy divided by elapsed years. Independent predictors of preeclampsia were past history of oligomenorrhea, WAR >0.8 kg/years, pre-pregnancy or 1st trimester triglyceridemia >150 mg/dL, and elevated acanthosis nigricans in the neck. In a multivariate logistic regression model, two or more predictors conferred an odds ratio of 15 (95%CI [5.9-37]; P < 0.001) to develop preeclampsia (85% specificity, 73% sensitivity, c-statistic of 81 ± 4%; P < 0.0001). Clinical markers related to insulin resistance and sedentary lifestyles are strong independent predictors of preeclampsia in mothers with 3rd trimester fetal losses or preterm deliveries due to placental insufficiency. Women at risk for preeclampsia in this particular population might benefit from measures focused on overcoming insulin resistance.

  2. Prevalence and Clinical Predictors of Insulin Resistance in Reproductive-Aged Thai Women with Polycystic Ovary Syndrome

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    Thanyarat Wongwananuruk

    2012-01-01

    Full Text Available Objectives. To determine the prevalence of insulin resistance (IR and its predictors in reproductive-aged Thai women with polycystic ovary syndrome (PCOS. Methods. A cross-sectional study was conducted from May 2007 to January 2009. Participants were 250 Thai women with PCOS. Information regarding medical history and physical examination and results of 75 gram OGTT were recorded. Results. The overall prevalence of IR was 20.0%, comprising the prevalence of impaired fasting glucose, impaired glucose tolerance, and diabetic mellitus of 3.2%, 13.6%, and 5.6%, respectively. Multiple logistic regression analysis showed that the independent predictors for IR were age of ≥30 years old, waist circumference (WC of ≥80 cm, presence of acanthosis nigricans (AN, and dyslipidemia with odds ratios (95% confidence interval of 2.14 (1.01–4.52, 3.53 (1.28–9.75, 2.63 (1.17–5.88, and 3.07 (1.16–8.11, respectively. Conclusion. The overall prevalence of IR in reproductive-aged Thai women with PCOS is 20.0%. Age ≥30 years old, WC ≥80 cm, the presence of AN, and dyslipidemia are the significant clinical predictors.

  3. Paraneoplastic hypertrichosis lanuginosa acquisita: uncommon or overlooked?

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    Slee, P H T J; van der Waal, R I F; Schagen van Leeuwen, J H; Tupker, R A; Timmer, R; Seldenrijk, C A; van Steensel, M A M

    2007-12-01

    Acquired hypertrichosis lanugo-type or hypertrichosis lanuginosa acquisita (HLA) is often associated with metabolic and endocrine disorders and use of certain drugs. The occurrence of HLA with malignancy was first noted in 1865, and it has since been described in 56 patients as a paraneoplastic syndrome both in women and in men. Sometimes HLA occurs concurrent with acanthosis nigricans, papillary hypertrophy of the tongue, and glossitis. The predominance of female cases is striking. Malignancy-associated HLA seems to occur especially in the age group 40-70 years. In women with HLA the most frequent malignancy is colorectal cancer, followed in order by lung cancer and breast cancer; in men lung cancer is the malignancy most frequently associated with HLA, followed by colorectal cancer. In 3 years we saw 10 patients with HLA, in whom the malignancy was usually metastasized. Only one patient had local disease; after removal of the primary tumour it took 2 years before the lanugo hair recurred. The aetiology of the syndrome is not clear: no specific hormonal or biochemical abnormalities have been identified as yet. The difference between hirsutism and lanugo-type hypertrichosis is discussed. It is stressed that the appearance of lanugo-type hypertrichosis in body areas previously perceived by patients as 'hairless' is highly indicative of internal malignancy.

  4. The skin landscape in diabetes mellitus. Focus on dermocosmetic management

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    Piérard, Gérald E; Seité, Sophie; Hermanns-Lê, Trinh; Delvenne, Philippe; Scheen, André; Piérard-Franchimont, Claudine

    2013-01-01

    Background Some relationships are established between diabetes mellitus (DM) and a series of cutaneous disorders. Specific dermatoses are markers for undiagnosed DM. Other disorders represent supervening complications in an already treated DM patient. Objective To review the information about dermocosmetic care products and their appropriate use in the management and prevention of dermatoses related to DM. Method The peer-reviewed literature and empiric findings are covered. Owing to the limited clinical evidence available for the use of dermocosmetics, a review of the routine practices and common therapies in DM-related dermatoses was conducted. Results Some DM-related dermatoses (acanthosis nigricans, pigmented purpuric dermatosis) are markers of macrovascular complications. The same disorders and some others (xerosis, Dupuytren’s disease) have been found to be more frequently associated with microangiopathy. Other skin diseases (alopecia areata, vitiligo) were found to be markers of autoimmunity, particularly in type 1 DM. Unsurprisingly, using dermocosmetics and appropriate skin care has shown objective improvements of some DM-related dermatoses, such effects improve the quality of life. The most common skin manifestations of DM fall along continuum between “dry skin,” xerosis, and acquired ichthyosis, occurring predominately on the shins and feet. Dermocosmetic products improve the feeling of well-being for DM patients. PMID:23696712

  5. Clinicopathological correlation of acquired hyperpigmentary disorders

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    Anisha B Patel

    2013-01-01

    Full Text Available Acquired pigmentary disorders are group of heterogenous entities that share single, most significant, clinical feature, that is, dyspigmentation. Asians and Indians, in particular, are mostly affected. Although the classic morphologies and common treatment options of these conditions have been reviewed in the global dermatology literature, the value of histpathological evaluation has not been thoroughly explored. The importance of accurate diagnosis is emphasized here as the underlying diseases have varying etiologies that need to be addressed in order to effectively treat the dyspigmentation. In this review, we describe and discuss the utility of histology in the diagnostic work of hyperpigmentary disorders, and how, in many cases, it can lead to targeted and more effective therapy. We focus on the most common acquired pigmentary disorders seen in Indian patients as well as a few uncommon diseases with distinctive histological traits. Facial melanoses, including mimickers of melasma, are thoroughly explored. These diseases include lichen planus pigmentosus, discoid lupus erythematosus, drug-induced melanoses, hyperpigmentation due to exogenous substances, acanthosis nigricans, and macular amyloidosis.

  6. 儿童肥胖与2型糖尿病相关疾病的研究进展

    Institute of Scientific and Technical Information of China (English)

    祁磊; 肖延风

    2007-01-01

    儿童肥胖与2型糖尿病(type 2 diabetes mellitus,T2DM)的发病密切相关,是T2DM最重要的危险因子.T2DM早期可出现一系列相关的症状和/或疾病,包括:胰岛素抵抗(insulin resistance,IR)、糖耐量减低(impaired glucose tolerance,IGT)、脂代谢紊乱、代谢综合征(metabolic syndrome,MS)、黑色棘皮病(acanthosis nigricans,AN)等,肥胖在这些疾病的发生发展中起了重要作用.本文从以上五个方面介绍了儿童肥胖与T2DM相关疾病的研究进展,为预防和降低肥胖儿童T2DM发病率提供了病因学参考.

  7. The skin function: a factor of anti-metabolic syndrome

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    Zhou Shi-Sheng

    2012-04-01

    Full Text Available Abstract The body’s total antioxidant capacity represents a sum of the antioxidant capacity of various tissues/organs. A decrease in the body’s antioxidant capacity may induce oxidative stress and subsequent metabolic syndrome, a clustering of risk factors for type 2 diabetes and cardiovascular disease. The skin, the largest organ of the body, is one of the major components of the body’s total antioxidant defense system, primarily through its xenobiotic/drug biotransformation system, reactive oxygen species-scavenging system, and sweat glands- and sebaceous glands-mediated excretion system. Notably, unlike other contributors, the skin contribution is variable, depending on lifestyles and ambient temperature or seasonal variations. Emerging evidence suggests that decreased skin’s antioxidant and excretory functions (e.g., due to sedentary lifestyles and low ambient temperature may increase the risk for metabolic syndrome. This review focuses on the relationship between the variability of skin-mediated detoxification and elimination of exogenous and endogenous toxic substances and the development of metabolic syndrome. The potential role of sebum secretion in lipid and cholesterol homeostasis and its impact on metabolic syndrome, and the association between skin disorders (acanthosis nigricans, acne, and burn and metabolic syndrome are also discussed.

  8. Correlation of Lipid Profile and Risk of Developing Type 2 Diabetes Mellitus in 10-14 Year Old Children

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    Nusrath M. Habiba

    2016-09-01

    Full Text Available Background/Aims: The role of lipid profile in predicting the risk of Type 2 diabetes mellitus (T2DM in children is not clearly established. Our aim is to screen non-diabetic children aged 10-14 years for risk of developing T2DM and evaluate the association of abnormal lipids and socioeconomic status (SES. Methods: Data on race/ethnicity, family history, body mass index percentile, blood pressure and presence of neck pigmentation (acanthosis nigricans were collected from 149 non-diabetic children. Using these factors, children were classified into low risk (3 risk factors groups. Logistic regression model and chi-square tests were used to evaluate the association of blood lipid profile and demographic variables. Independent t-test was used to compare the ratio of Total Cholesterol (TC and High Density Lipids (HDL with T2DM risk. Results: 60% of children were at high risk for developing T2DM. HDL (pp=0.02 and TC/HDL ratio (pConclusions: The significant determinants associated with high risk group were modifiable factors providing an opportunity for early intervention and prevention.

  9. A STUDY ON PATTERN OF SKIN DISEASES AMONG CHILDREN PRESENTING TO RIMS, KADAPA, A. P.

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    Vamseedhar

    2015-08-01

    Full Text Available BACKGROUND: Little information is available about the prevalence of skin conditions among children in the general population in India. Low socioeconomic status, malnutrition, overcrowding and poor standards of hygiene are important factors accounting for the distribut ion of skin diseases in developing countries such as India. AIM OF THE STUDY: To study prevalence of skin diseases among children presenting to paediatric OPD. METERIALS AND METHODS: A predesigned and pretested questionnaire was used to gather information about socio - demographic profile, personal history, social history and all the children were thoroughly examined by detailed clinical examination depending on signs and symptoms. Suspected cases were subjected to bacterial cultures, KoH examination was done . RESULTS: In the present study, pyoderma was the most common frequent skin disorder accounting to 11.2% followed by Scabies 10.6%, Papular urtricaria 8.8%, Impetigo 6.8% and the least prevalent were Measles 0.8%, Acanthosis nigricans 0.4% and Psoriasis 0. 2%. CONCLUSION: In the present study pyoderma, utericaria and scabies were highly prevalent among children’s. Personal hygine, health education and periodic deworming measures will prevent majority of skin diseases. KEYWORDS: S kin diseases, C hildren, P yode rma, S cabies.

  10. Genealogy, natural history, and phenotype of Alström syndrome in a large Acadian kindred and three additional families.

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    Marshall, J D; Ludman, M D; Shea, S E; Salisbury, S R; Willi, S M; LaRoche, R G; Nishina, P M

    1997-12-12

    We describe a large Acadian kindred including 8 Alstrom Syndrome (AS) patients, with an age range of 4 to 26 at the time of clinical assessment. The affected subjects come from 5 nuclear families within this kindred. The phenotype includes early childhood retinopathy, progressive sensorineural hearing loss, truncal obesity, and acanthosis nigricans. In addition, hyperinsulinemia and hypertriglyceridemia with normal cholesterol levels were observed in most affected individuals tested. Non-insulin dependent diabetes mellitus and growth retardation appear to be age-related manifestations that occur post-adolescence. Younger affected children are not overtly hyperglycemic and are normal or above average height for age. Although the AS patients in kindred 1 presumably carry the same mutation, many manifestations of the disease are variable. For example, of the 8 children in the Acadian kindred, 4 have scoliosis, 2 have had infantile cardiomyopathy, 2 are hypothyroid, 1 has had hepatic dysfunction and is hypertensive, and 4 have developed asthma. Seven subjects described in this kindred exhibit developmental delay. One additional manifestation not described widely in the literature, advanced bone age, was observed in all subjects tested. The clinical data from this large Acadian kindred, together with information obtained from 4 additional AS patients in 3 unrelated kindreds, confirm and extend clinical observations previously described. In addition, the Acadian kindred with multiple affected individuals, probably arising from a common founder, should allow for identification of the chromosomal localization of a gene causing AS.

  11. What every physician should know about polycystic ovary syndrome.

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    Rosenfield, Robert L

    2008-01-01

    Polycystic ovary syndrome (PCOS) is the most common endocrine cause of hirsutism, acne, and pattern alopecia. It is a heterogeneous syndrome of hyperandrogenic anovulation that is typically due to intrinsic ovarian dysfunction, which is often aggravated by insulin-resistant hyperinsulinemia with its risks of diabetes mellitus and metabolic syndrome and their complications. Because there are many pitfalls to androgen assays, evaluation for hyperandrogenemia is suggested in women with moderate or severe hirsutism or hirsutism equivalents, menstrual irregularity, acanthosis nigricans, or intractable obesity. An endocrinologic work-up is necessary to rule out other hyperandrogenic disorders that require specific therapy (e.g., virilizing tumors, nonclassic congenital adrenal hyperplasia, hyperprolactinemia, and Cushing's syndrome). Ultrasonography helps in the differential diagnosis and may demonstrate the polycystic ovaries that have recently been vetted as an alternative to oligo-anovulation as a diagnostic criterion. Management of PCOS is determined by symptomatology. For those women not desiring pregnancy, the most common therapies are oral contraceptive pills, antiandrogens (contraindicated in the absence of adequate contraception), and insulin-lowering treatments (which have little effect on hirsutism).

  12. The Alström syndrome: is it a rare or unknown disease?

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    Maffei, Pietro; Munno, Vincenzo; Marshall, Jan D; Scandellari, Cesare; Sicolo, Nicola

    2002-01-01

    The Alström syndrome is a rare, autosomal recessive disorder characterized by retinal degeneration, obesity, progressive hearing impairment, non-insulin-dependent diabetes mellitus and kidney and heart failure. Mental retardation is absent and the extremities are normal. The Alström syndrome gene located on chromosome 2, has been recently identified. The Alström syndrome involves multiple organ systems with a complex interaction between pathways. Phenotypic expression varies considerably, even within sibships. Manifestations observed in some, but not all, Alström syndrome patients include acanthosis nigricans, alopecia, short stature, scoliosis, kyphosis, hyperostosis frontalis interna, muscle dystonia, advanced bone age and subcapsular cataract. Other metabolic and endocrinological abnormalities have been described: hypothyroidism, hypogonadism, diabetes insipidus, growth hormone deficiency, hyperuricemia and hyperlipidemia. In the final stages of the disease, affected individuals exhibit progressive chronic nephropathy with eventual kidney failure. The most frequent causes of death include hepatic dysfunction and congestive heart failure secondary to dilated cardiomyopathy. We have summarized our personal clinical data and the information from the scientific literature on the topic in order to provide an up-to-date review on the Alström syndrome.

  13. Clinical characteristics of polycystic ovary syndrome in Indian women

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    Sunita J Ramanand

    2013-01-01

    Full Text Available Background: Polycystic ovary syndrome (PCOS is common diagnosis in women presenting with infertility. All the dimensions of PCOS have not been completely explored. Many studies have tried to characterize the exact presentation of the disease. In this study we studied clinical features of PCOS in Indian women to characterize different phenotypes of this syndrome. Prevalence of acanthosis nigricans (AN as surrogate marker of insulin resistance, obesity, hirsutism and hypothyroidism in PCOS women have been simultaneously studied. Materials and Methods: Present work is a non comparative cross-sectional open label study carried out over a period of 18 months in an endocrinology hospital in western Maharashtra, India. Results and Conclusion: Authors conclude that PCOS occurs both in obese and non-obese women; AN and hirsutism occur in equal proportion of patients. AN is correlated with obesity. Hormonal dysfunctions in PCOS manifested together or independently. PCOS women can be sub grouped based on clinical features suggestive of endocrinological malfunctions and can be investigated accordingly for selection of appropriate treatment modalities.

  14. A Case of Congenital Adrenal Hyperplasia Mimicking Cushing's Syndrome

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    Kim, Hye Jeong; Kang, Mira; Kim, Jae Hyeon; Kim, Sun Wook; Chung, Jae Hoon; Min, Yong-Ki; Lee, Moon-Kyu; Kim, Kwang-Won

    2012-01-01

    Congenital adrenal hyperplasia (CAH) is characterized by decreased adrenal hormone production due to enzymatic defects and subsequent rise of adrenocorticotrophic hormone that stimulates the adrenal cortex to become hyperplastic, and sometimes tumorous. As the pathophysiology is basically a defect in the biosynthesis of cortisol, one may not consider CAH in patients with hypercortisolism. We report a case of a 41-yr-old man with a 4 cm-sized left adrenal tumorous lesion mimicking Cushing's syndrome who was diagnosed with CAH. He had central obesity and acanthosis nigricans involving the axillae together with elevated 24-hr urine cortisol level, supporting the diagnosis of Cushing's syndrome. However, the 24-hr urine cortisol was suppressed by 95% with the low dose dexamethasone suppression test. CAH was suspected based on the history of precocious puberty, short stature and a profound suppression of cortisol production by dexamethasone. CAH was confirmed by a remarkably increased level of serum 17-hydroxyprogesterone level. Gene mutation analysis revealed a compound heterozygote mutation of CYP21A2 (I173N and R357W). PMID:23166432

  15. Type 2 diabetes mellitus in children--an increasing health problem in Mexico.

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    Cruz, Miguel; Torres, Margarita; Aguilar-Herrera, Blanca; Pérez-Johnston, Rocío; Guzmán-Juárez, Nora; Aranda, Martha; Kumate, Jesús

    2004-02-01

    The incidence of type 2 diabetes mellitus (DM2) in children has increased worldwide and is commonly associated with overweight. Forty-four children with DM2 were studied by clinical histories, anthropometric measurements, and biochemical analysis. Homeostasis model assessment (HOMA-IR) and quantitative insulin sensitivity check index (QUICKI) were determined to evaluate insulin resistance. Only five patients presented normal body mass index (BMI); the remainder were overweight, and 76% had acanthosis nigricans. Laboratory results yielded hyperglycemia, elevated glycosylated hemoglobin, insulin and C-peptide. Elevated HOMA-IR and decreased QUICKI values suggest insulin resistance. No significant difference was found between sexes, although overweight in girls had more influence over blood pressure and lipid levels (p <0.05). Time from diagnosis and HOMA-IR yielded relevant values (p = 0.010). Laboratory results, QUICKI, and HOMA-IR values suggested that these patients present DM2 and decreased insulin sensitivity. We recommend prevention of overweight and sedentary life-style.

  16. Cardiovascular risk factors and events in women with androgen excess.

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    Macut, D; Antić, I B; Bjekić-Macut, J

    2015-03-01

    Androgen excess (AE) was approximated to be present in 7% of the adult population of women. Polycystic ovary syndrome (PCOS) is the most prevalent among them, followed by idiopathic hirsutism (IH), congenital adrenal hyperplasia (CAH), hyperandrogenic insulin-resistant acanthosis nigricans (HAIRAN) syndrome, and androgen-secreting neoplasms (ASNs). Increased cardiovascular risk was implicated in women with AE. Serum testosterone independently increases risk for cardiovascular disease (CVD), and correlates even with indices of subclinical atherosclerosis in various populations of postmenopausal women. Hyperandrogenism in PCOS is closely related to the aggravation of abdominal obesity, and together with insulin resistance forming the metabolic core for the development of CVD. However, phenotypic variability of PCOS generates significant influence on the cardiometabolic risks. Numerous risk factors in PCOS lead to 5-7 times higher risk for CVD and over 2-fold higher risk for coronary heart disease and stroke. However, issue on the cardiometabolic risk in postmenopausal women with hyperandrogenic history is still challenging. There is a significant overlapping in the CVD characteristics of women with PCOS and variants of CAH. Relevant clinical data on the prevalence and cardiometabolic risk and events in women with IH, HAIRAN syndrome or ASNs are scarce. The effects of various oral contraceptives (OCs) and antiandrogenic compounds on metabolic profile are varying, and could be related to the selected populations and different therapy regiments mainly conducted in women with PCOS. It is assumed relation of OCs containing antiandrogenic progestins to the increased risk of cardiovascular and thromboembolic events.

  17. Black hairy tongue syndrome.

    Science.gov (United States)

    Gurvits, Grigoriy E; Tan, Amy

    2014-08-21

    Black hairy tongue (BHT) is a benign medical condition characterized by elongated filiform lingual papillae with typical carpet-like appearance of the dorsum of the tongue. Its prevalence varies geographically, typically ranging from 0.6% to 11.3%. Known predisposing factors include smoking, excessive coffee/black tea consumption, poor oral hygiene, trigeminal neuralgia, general debilitation, xerostomia, and medication use. Clinical presentation varies but is typically asymptomatic, although aesthetic concerns are common. Differential diagnosis includes pseudo-BHT, acanthosis nigricans, oral hairy leukoplakia, pigmented fungiform papillae of the tongue, and congenital melanocytic/melanotic nevi/macules. Clinical diagnosis relies on visual observation, detailed history taking, and occasionally microscopic evaluation. Treatment involves identification and discontinuation of the offending agent, modifications of chronic predisposing factors, patient's re-assurance to the benign nature of the condition, and maintenance of adequate oral hygiene with gentle debridement to promote desquamation. Complications of BHT (burning mouth syndrome, halitosis, nausea, gagging, dysgeusia) typically respond to therapy. Prognosis is excellent with treatment of underlying medical conditions. BHT remains an important medical condition which may result in additional burden on the patient and health care system and requires appropriate prevention, recognition and treatment.

  18. FGFR2 abnormalities underlie a spectrum of bone, skin, and cancer pathologies.

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    Katoh, Masaru

    2009-08-01

    Fibroblast growth factor receptor (FGFR)2 is regulated on the basis of the balance of FGFs, heparan-sulfate proteoglycans, FGFR2 isoforms, endogenous inhibitors, and microRNAs. FGFR2 signals cross-talk with hedgehog, bone morphogenetic protein, and other regulatory networks. Some cases of congenital skeletal disorders with an FGFR2 mutation show skin phenotypes, including acne, cutis gyrata, and acanthosis nigricans. Gain-of-function mutations or variations of human FGFR2 occur in estrogen receptor-positive breast cancer, diffuse-type gastric cancer, and endometrial uterine cancer. Oral administration of AZD2171 or Ki23057 inhibits in vivo proliferation of cancer cells with aberrant FGFR2 activation in rodent therapeutic models. However, loss-of-function mutations of FGFR2 are reported in human melanoma. Conditional Fgfr2b knockout in the rodent epidermis leads to increased macrophage infiltration to the dermis and adipose tissue, epidermal thickening accompanied by basal-layer dysplasia and parakeratosis, and the promotion of chemically induced squamous-cell carcinoma. Dysregulation of FGFR2 results in a spectrum of bone and skin pathologies and several types of cancer.

  19. Você conhece esta síndrome? Do you know this syndrome?

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    Júnia Bicalho de Sousa

    2006-02-01

    Full Text Available A lipodistrofia generalizada congênita (síndrome de Berardinelli-Seip, doença autossômica recessiva, caracteriza-se por escassez do tecido subcutâneo. A falta de tecido adiposo propicia disfunção metabólica dos lípides e carboidratos, resistência periférica à insulina, hipertrigliceridemia e hipermetabolismo. Outros achados são acantose nigricante, acromegalia, hepatomegalia e alterações musculares, ósseas, cardiovasculares e neurológicas. Relata-se o caso de paciente com essa síndrome, cujo diagnóstico foi realizado em um serviço de dermatologia.Congenital generalized lipodystrophy (Berardinelli-Seip syndrome is a recessive autossomic disease characterized by absence of subcutaneous tissue. The fat tissue absence leads to metabolic dysfunction of lipids and carbohydrates, peripheral insulin resistance and increased seric levels of triglycerides and also a higher metabolic rate. Other findings are acanthosis nigricans, acromegaly, hepatomegaly and muscular, bony, cardiovascular and neurological abnormalities. A case of a patient with this syndrome is reported, whose diagnosis was made in a dermatology ambulatory.

  20. Predicting Metabolic Syndrome in Obese Children and Adolescents: Look, Measure and Ask

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    Nicola Santoro

    2013-02-01

    Full Text Available Objective: To verify in obese children whether or not the presence of i high waist-to-height ratio (WHtR, ii family history for type 2 diabetes (T2D and iii acanthosis nigricans (AN, singularly or together, might predict the occurrence of metabolic syndrome or prediabetes. Methods. 1,080 Italian obese children (567 females were enrolled. Blood pressure, fasting plasma glucose, insulin, and lipids were measured, and oral glucose tolerance test (OGTT was performed. The WHtR was calculated, family history for T2D was assessed, and the presence of AN was noticed. The odds ratios for showing metabolic syndrome and/or prediabetes according to the presence of these features were calculated. Results: The prevalence of metabolic syndrome was 29.2%. AN (OR1.81; p = 0.002 and WHtR higher than 0.60 (OR 2.24; p Conclusions: Three simple actions, i.e., looking at the patient, asking about T2D family history, and measuring WHtR, may represent a powerful tool in the hands of pediatricians to identify obese children with high cardiovascular and metabolic risk.

  1. Hirsutism: Clinico-investigative profile of 50 Indian patients

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    Sharma Nand

    2008-01-01

    Full Text Available Background: Despite worldwide prevalence of hirsutism studies on hirsutism in Indian patients are not many. Aims: This retrospective study was carried out to assess the clinico-investigative profile of patients presenting with hirsutism. Materials and Methods: Medical records of 82 hirsutism patients diagnosed consecutively during July 2005 to October 2007 were analyzed. Results: The complete data of 50 patients aged between 13 and 47 years were available. Fifty percent patients were aged 20 to 30 years. The average F-G score was 10.3 ± 2.46. Associated signs of hyperandrogenism were acne (64%, oligomenorrhea or menstrual irregularities (36%, androgenetic alopecia (16%, acanthosis nigricans (6% and seborrhea (4%. Polycystic ovaries were detected in 30% patients and 22% patients had elevated serum free testosterone levels. Family history of hirsutism was present in 18% patients. Conclusion: Hirsutism in Indian patients is not uncommon. Adolescent patients appear to be more concerned about hirsutism as compared to those in the older age group who were more often worried of late onset acne. All patients, however, were more concerned for facial hair than those on other body areas signifying that facial hair need to be given higher than current value in F-G score.

  2. A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy

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    Damien Galant

    2016-04-01

    Full Text Available ZMPSTE24 encodes the only metalloprotease, which transforms prelamin into mature lamin A. Up to now, mutations in ZMPSTE24 have been linked to Restrictive Dermopathy (RD, Progeria or Mandibulo-Acral Dysplasia (MAD. We report here the phenotype of a patient referred for severe metabolic syndrome and cardiomyopathy, carrying a mutation in ZMPSTE24. The patient presented with a partial lipodystrophic syndrome associating hypertriglyceridemia, early onset type 2 diabetes, and android obesity with truncal and abdominal fat accumulation but without subcutaneous lipoatrophy. Other clinical features included acanthosis nigricans, liver steatosis, dilated cardiomyopathy, and high myocardial and hepatic triglycerides content. Mutated fibroblasts from the patient showed increased nuclear shape abnormalities and premature senescence as demonstrated by a decreased Population Doubling Level, an increased beta-galactosidase activity and a decreased BrdU incorporation rate. Reduced prelamin A expression by siRNA targeted toward LMNA transcripts resulted in decreased nuclear anomalies. We show here that a central obesity without subcutaneous lipoatrophy is associated with a laminopathy due to a heterozygous missense mutation in ZMPSTE24. Given the high prevalence of metabolic syndrome and android obesity in the general population, and in the absence of familial study, the causative link between mutation and phenotype cannot be formally established. Nevertheless, altered lamina architecture observed in mutated fibroblasts are responsible for premature cellular senescence and could contribute to the phenotype observed in this patient.

  3. High blood pressure in school children: prevalence and risk factors

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    Rivers Patrick A

    2006-11-01

    Full Text Available Abstract Background The purpose of this study was to determine the prevalence of high blood pressure (HBP and associated risk factors in school children 8 to 13 years of age. Methods Elementary school children (n = 1,066 were examined. Associations between HBP, body mass index (BMI, gender, ethnicity, and acanthosis nigricans (AN were investigated using a school based cross-sectional study. Blood pressure was measured and the 95th percentile was used to determine HBP. Comparisons between children with and without HBP were utilized. The crude and multiple logistic regression adjusted odds ratios were used as measures of association. Results Females, Hispanics, overweight children, and children with AN had an increased likelihood of HBP. Overweight children (BMI ≥ 85th percentile and those with AN were at least twice as likely to present with HBP after controlling for confounding factors. Conclusion Twenty one percent of school children had HBP, especially the prevalence was higher among the overweight and Hispanic group. The association identified here can be used as independent markers for increased likelihood of HBP in children.

  4. Markers of insulin resistance and sedentary lifestyle are predictors of preeclampsia in women with adverse obstetric results

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    S. Hoirisch-Clapauch

    2011-12-01

    Full Text Available Some thrombophilias and severe preeclampsia may increase the risk for preterm deliveries and fetal death due to placental insufficiency. Our objective was to evaluate clinical and laboratory data as predictors of preeclampsia in a population of mothers with 3rd trimester fetal losses or preterm deliveries. In a longitudinal retrospective study, 54 consecutive women (age range: 16 to 39 years with normotensive pregnancies were compared to 79 consecutive women with preeclampsia (age range: 16 to 43 years. Weight accrual rate (WAR was arbitrarily defined as weight gain from age 18 years to the beginning of pregnancy divided by elapsed years. Independent predictors of preeclampsia were past history of oligomenorrhea, WAR >0.8 kg/years, pre-pregnancy or 1st trimester triglyceridemia >150 mg/dL, and elevated acanthosis nigricans in the neck. In a multivariate logistic regression model, two or more predictors conferred an odds ratio of 15 (95%CI [5.9-37]; P < 0.001 to develop preeclampsia (85% specificity, 73% sensitivity, c-statistic of 81 ± 4%; P < 0.0001. Clinical markers related to insulin resistance and sedentary lifestyles are strong independent predictors of preeclampsia in mothers with 3rd trimester fetal losses or preterm deliveries due to placental insufficiency. Women at risk for preeclampsia in this particular population might benefit from measures focused on overcoming insulin resistance.

  5. Acne - a potential skin marker of internal disease.

    Science.gov (United States)

    Pace, Joseph L

    2015-01-01

    Polycystic ovary syndrome (PCOS) is the most prevalent endocrine disorder in adult women. Hyperandrogenism is the crux of the pathogenesis of both acne and hirsutism, the most frequent clinical presentations of the syndrome. The chronic anovulation that may occur, often but not always associated with enlarged cystic ovaries, has long been recognized as an important feature of PCOS. In recent years major changes have occurred with regard to PCOS: Although management of the common cutaneous manifestations, mainly acne, hirsutism, alopecia, and acanthosis nigricans, remains strictly within the realm of daily dermatologic practice, the pendulum is shifting toward greater awareness of the longer-term systemic implications of PCOS, with emphasis on the unique opportunity and privileged position of the dermatologist to diagnose this potentially serious problem at an early stage, when effective long-term treatment can be instituted. Patients need to be advised that PCOS cannot be cured but can be controlled. Management should involve a multidisciplinary team with emphasis on lifestyle change, insulin sensitizing agents, androgen blockers, and attention to specific cutaneous manifestations.

  6. Dermatologic manifestations of polycystic ovary syndrome.

    Science.gov (United States)

    Lee, Amy T; Zane, Lee T

    2007-01-01

    Polycystic ovary syndrome (PCOS) affects 5-10% of reproductive-aged women and is one of the most common endocrine disorders in women. The disorder is commonly characterized by elevated levels of androgen and insulin. Women with PCOS may present with a range of signs and symptoms, and face increased risks of reproductive, metabolic, cardiovascular, psychologic, and neoplastic sequelae, particularly if the condition is left unrecognized or untreated. The clinical definition of PCOS has changed in recent years and includes as one of its cardinal criteria the dermatologic manifestations of hyperandrogenism, chiefly hirsutism, acne vulgaris, and androgenetic alopecia. Acanthosis nigricans, a cutaneous sign of hyperinsulinemia, may also be present. These dermatologic features may provide early clinical clues to recognition of PCOS, and treatment of these cutaneous conditions may improve the patient's quality of life and psychologic well-being. The effects of androgen on pilosebaceous units in the skin can vary by anatomic location, producing pathophysiologic effects on hair growth and differentiation, sebaceous gland size and activity, and follicular keratinization. Treatment modalities may include hormonal therapy intended to modulate androgen production and action as well as non-hormonal therapies directed toward specific dermatologic conditions.

  7. Navigating Adolescence with a Chronic Health Condition: A Perspective on the Psychological Effects of HAIR-AN Syndrome on Adolescent Girls

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    Kimberly K. McClanahan

    2006-01-01

    Full Text Available HAIR-AN syndrome is a subphenotype of polycystic ovary syndrome and is characterized by acne, obesity, hirsutism, and acanthosis nigricans. It usually manifests in early adolescence, a time of significant developmental change in females across physical, cognitive, social, and emotional domains. We contend that adolescent development for females is difficult, even in the best of circumstances, and having a chronic health condition, like HAIR-AN syndrome, will likely impact the afflicted individual’s development and psychological well-being. While many researchers have discussed the long-term health effects of HAIR-AN and similar disorders, little has been written about the potential psychological sequelae of HAIR-AN on the adolescent girl. We discuss the normal developmental sequence for adolescent girls across early, middle, and late adolescence; discuss common mental health problems that adolescents experience; define HAIR-AN syndrome and its clinical manifestations; and discuss its likely psychological impact on adolescent girls. We also make suggestions for future clinical interventions and research in the area of HAIR-AN syndrome and its psychological sequelae.

  8. Clinical Profiles, Occurrence, and Management of Adolescent Patients with HAIR-AN Syndrome

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    Hatim A. Omar

    2004-01-01

    Full Text Available The syndrome of hyperandrogenism, insulin resistance, and acanthosis nigricans (HAIR-AN is a subphenotype of the polycystic ovary syndrome. It is one of the most common causes of menstrual problems, hyperandrogenic symptoms, and insulin resistance among young women. Review of clinical data in an outpatient adolescent clinic showed that of the 1,002 young women (ages 10—21 years attending the clinic over a 2-year period, 50 (5% were diagnosed with HAIR-AN syndrome. Mean age of the patients was 15.5, initial mean weight at diagnosis was 94.5 kg, and the mean BMI was 33.33 kg/m2. Patients were treated with a weight-stabilization and -reduction program, oral contraceptive pills, and in most cases metformin. Of the patients, 80% were compliant with the follow-up and treatment regimen, 60% maintained or reduced their weight, 95% had regular menstrual cycles, and in most patients, the acne and/or hirsutism were the same or better than at the start of treatment. We conclude that HAIR-AN syndrome is a common disease in young women and multifaceted, aggressive treatment appears to be effective in reducing the severity of symptoms and preventing further consequences.

  9. O impacto da obesidade sobre os componentes da síndrome metabólica e as adipocitoquinas em crianças pré-púberes Impact of obesity on metabolic syndrome components and adipokines in prepubertal children

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    Isabel R. Madeira

    2009-06-01

    Full Text Available OBJETIVO: Verificar o impacto da obesidade sobre os componentes da síndrome metabólica e sobre os níveis de adipocitoquinas em crianças pré-púberes. MÉTODOS: Estudo transversal comparando 30 crianças obesas, 31 com sobrepeso e 33 eutróficas, oriundas do ambulatório de pediatria geral de um hospital universitário, quanto às médias de glicose, lipídios séricos, insulina, HOMA-IR (homeostasis model assessment-insulin resistance, relação glicose/insulina, adiponectina e leptina. Compararam-se as frequências de acantose nigricans e das alterações de cintura, pressão arterial, glicose, lipídios séricos e insulina. Avaliou-se a correlação entre escore z de índice de massa corporal (IMC e adipocitoquinas. RESULTADOS: Houve diferença nas médias dos obesos, quanto a HDL-colesterol e adiponectina, e nas dos eutróficos, quanto a insulina, HOMA-IR, relação glicose/insulina e leptina (p OBJECTIVE: To verify the impact of obesity on metabolic syndrome components and adipokine levels in prepubertal children. METHODS: This cross-sectional study compared 30 obese, 31 overweight and 33 eutrophic children attending a university hospital-based outpatient pediatric clinic. Parameters assessed included glucose, serum lipids, insulin, homeostasis model assessment-insulin resistance (HOMA-IR, glucose/insulin relation, adiponectin, and leptin. We compared the frequency of acanthosis nigricans and changes in waist, blood pressure, glucose, serum lipids, and insulin. The correlation between body mass index (BMI z score and adipokines was evaluated. RESULTS: Among obese children, there was a difference in the mean values of HDL cholesterol and adiponectin, whereas among the eutrophic children, there was a difference in the mean values of insulin, HOMA-IR, glucose/insulin relation, and leptin (p < 0.001. A difference was also observed regarding the frequency of acanthosis nigricans and alteration in waist and HDL cholesterol (p < 0.005 in the

  10. [Habitat use patterns of the Black Brant Branta bernicla nigricans (Anseriformes: Anatidae) in natural and artificial areas of Guerrero Negro, Baja California Sur, Mexico].

    Science.gov (United States)

    Cedillo, Israel Martínez; Carmona, Roberto; Ward, David H; Danemann, Gustavo D

    2013-06-01

    The Black Brant is a common inhabitant of the Western Artic American tundra, which migrates to Southern Pacific coasts during the winter season. Approximately, 31000 birds (31%) constitute the Mexican population of Brants at Guerrero Negro, Ojo de Liebre, and Exportadora de Sal lagoon complex; nevertheless, there is little information about the distribution patterns and zone usage. At Guerrero Negro Lagoon (GNL), Ojo de Liebre Lagoon (OLL, both natural sites), and at Exportadora de Sal (ESSA, artificial site) we determined by monthly censuses (from November 2006 to April 2007, 08:00-16:00h) and observed: (1) season and site effects on population structure (age groups), and (2) the tide level relationship with the abundance and proportion of feeding birds. Within a total of 150 observation hours and 98 birds, our results showed a general 0.68 proportion of adults, that was higher in winter than in spring. The statistics analysis showed no effects by site on the proportion of feeding birds, but we observed a temporal decrease at ESSA and at GNL. In contrast the proportion of feeding birds at OLL was constant. We observed an increase in the juveniles between winter and spring. This increase is related with the differential migration, which mentions that the juveniles are the last to leave the wintering area. In winter the relations of the tide level with the abundance of Brant were: direct at ESSA, inverse at OLL and no relation found at GNL. In spring, no relation was observed in the sites. The proportion of Brants feeding at OLL (the site with the higher abundance) was independent of the tide level. This is related with two possible behaviors of the geese: (1) they can move through the lagoon and take advantage of the tidal lag, which is up to four hours; and (2) they can modify their feeding strategies, more on floating eelgrass (Zostera marina).

  11. Lipodistrofia generalizada congênita Congenital generalized lipodystrophy

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    Paulo P. Figueiredo Filho

    2004-08-01

    Full Text Available OBJETIVO: Apresentar as principais características clínicas e bioquímicas da lipodistrofia generalizada congênita, desordem rara e pouco conhecida dos pediatras. DESCRIÇÃO: Nos ambulatórios de Doenças Nutricionais e de Endocrinologia do Serviço de Pediatria do Hospital das Clínicas da UFMG, foram identificados oito pacientes com lipodistrofia generalizada congênita. As características clínicas comuns a todos os casos foram hipertrofia muscular, lipoatrofia generalizada e aparência acromegálica. Manifestações clínico-laboratoriais associadas incluíram acantose nigricans em cinco pacientes, hepatoesplenomegalia em seis, hipertrigliceridemia com baixas concentrações de HDL em sete, hipertrofia cardíaca em um e diabetes melito secundário em dois pacientes. Todos os pacientes estão em controle clínico e dietético, visando à correção ou prevenção dos distúrbios metabólicos. COMENTÁRIOS: As características fenotípicas da lipodistrofia generalizada congênita são bem identificadas, possibilitando o diagnóstico clínico na maioria dos casos. Trata-se de uma síndrome rara que ilustra a importância do funcionamento normal do tecido adiposo para a maioria dos processos metabólicos vitais do organismo. O seu melhor conhecimento poderá abrir novos horizontes em estudos de doenças mais prevalentes como o diabetes melito e a obesidade.OBJECTIVE: To present the major clinical and biochemical characteristics of congenital generalized lipodystrophy. DESCRIPTION: Eight infants with congenital generalized lipodystrophy were identified at the Endocrine and Nutritional Pediatric Disease Outpatient Clinics at Hospital de Clínicas, Universidade Federal de Minas Gerais (UFMG. Clinical manifestations common to all patients included muscle hypertrophy, generalized lipoatrophy, and acromegalic physical appearance. Acanthosis nigricans was identified in five patients, hepatosplenomegaly in six, hypertriglyceridemia and low levels

  12. Diabetes melito do tipo 2 na infância e adolescência: revisão da literatura Type 2 diabetes in children and adolescents: literature review

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    Monica Gabbay

    2003-06-01

    Full Text Available OBJETIVO: análise crítica dos estudos sobre a epidemiologia, fisiopatologia, diagnóstico e tratamento do DM2 no jovem. MÉTODOS: revisão da literatura nos últimos 10 anos, através de pesquisa no banco de dados Medline, utilizando os termos "diabetes do tipo 2 no jovem". RESULTADOS: a fisiopatologia do DM2 no jovem é semelhante à do adulto, e compreende tanto a resistência à ação da insulina como uma alteração na função da célula beta-pancreática. O antecedente familiar para DM2, a presença de obesidade, a acanthosis nigricans, o peptídeo C de jejum superior a 0,6 ng/ml, a ausência de auto-anticorpos antiilhotas pancreáticas, em combinações variáveis, são pontos importantes para o diagnóstico desse tipo de DM. Cinco a 25% dos jovens com esse tipo de DM podem apresentar cetoacidose no diagnóstico. Nesses pacientes, o tratamento inicial com insulina é possível de ser descontinuado durante a evolução. A aderência à dieta e ao exercício físico são os elementos mais importantes do tratamento destes adolescentes. CONCLUSÃO: como a obesidade nos jovens tem aumentado, tanto em países industrializados como nos países em industrialização, o DM2 no jovem pode ser considerado um problema emergente também na nossa população.OBJECTIVE: the objective of this manuscript was to perform a critical review of epidemiology, pathophysiology, diagnosis e treatment of T2DM in youth. SOURCES OF DATA: this review is based on the relevant literature published. The sources available for the authors were integrated with sources identified through Medline database. The key words used for searching were "Type 2 Diabetes in the Youth" in the last ten years. SUMMARY OF THE FINDINGS: the pathophysiology (altered beta-cell function and insulin resistance of T2DM in youth is similar to adult's pathophysiology. Familiar Type 2 diabetes history, presence of obesity, acanthosis nigricans, high fasting plasma C-peptide levels and absence of

  13. Cutaneous manifestations of endocrine disorders: a guide for dermatologists.

    Science.gov (United States)

    Jabbour, Serge A

    2003-01-01

    diabetes mellitus include necrobiosis lipoidica diabeticorum, diabetic dermopathy, scleredema adultorum and acanthosis nigricans.

  14. Skin, a mirror reflecting diabetes mellitus: A longitudinal study in a tertiary care hospital in Gujarat

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    Roshni Vahora

    2013-01-01

    Full Text Available Context: Diabetes mellitus (DM is the most common of the endocrine disorders. Mucocutaneous manifestations of diabetes mellitus are many and vary from trivial to life-threatening. Sometimes, mucocutaneous disorders may herald the onset of diabetes. Aims: To study the pattern of mucocutaneous manifestations in diabetics and role of it in diagnosing diabetes mellitus and its complications. Settings and Design: It was a longitudinal observational study of patients having diabetes with skin complaints attending skin outdoor department or admitted in wards for any reason in a tertiary care hospital. Materials and Methods: Total 300 patients were included in the study. Detailed history, clinical examination, and relevant investigations were done to diagnose the mucocutaneous disorders, diabetes, and diabetic complications. Statistical Analysis Used: The data was analyzed by using Epi info software. Results: Demographic profile shown majority of cases (78.66% in more than 40 years of age with almost equal male and female preponderance. Mucocutaneous manifestations as presenting feature of diabetes were observed in 21.67% cases. Infections were most common in 119 (39.66% cases, followed by acanthosis nigricans in 46 (15.33% cases. Various associated complications like hypertension, retinopathy, hyperlipidemia, coronary artery disease, neuropathy, nephropathy, and diabetic ketoacidosis were observed in 160 (53.3%. Conclusions: Skin is the mirror, which reflects internal diseases; this aptly applies to skin and diabetes mellitus. Through awareness about cutaneous manifestations of DM, dermatologist can not only take credit for detecting DM but also facilitate early diagnosis of systemic complications of DM. This is immensely beneficial to patients in long run.

  15. Correlation between serum adiponectin and clinical characteristics, biochemical parameters in Indian women with polycystic ovary syndrome

    Science.gov (United States)

    Ramanand, Sunita J.; Ramanand, Jaiprakash B.; Ghongane, Balasaheb B.; Patwardhan, Milind H.; Patwardhan, Varsha M.; Ghanghas, Ravi; Halasawadekar, Nimish R.; Patil, Praveenkumar

    2014-01-01

    Background: Polycystic ovary syndrome (PCOS) is a common disorder. PCOS women are at a high risk for insulin resistance and metabolic syndrome (MS). Adiponectin is positively related to insulin sensitivity. It has a preventive role in atherogenesis and MS. The present work was conducted to study the correlation between serum adiponectin levels and clinical characteristics and biochemical parameters in PCOS patients. Materials and Methods: A prospective study in 49 newly diagnosed (as per Rotterdam criteria) Indian PCOS women was conducted. PCOS women were clinically examined and investigated for biochemical parameters. Results: The mean serum adiponectin was 12 ± 9.4 μg/mL (range 0.47-45). Hypoadiponectinemia (serum adiponectin <4 μg/mL) was present in 22% patients. Age and adiponectin correlated significantly and inversely (r = −0.42, P = 0.027). Overweight/obese patients had lower mean adiponectin levels than normal weight (11.62 ± 9.5 vs 13.58 ± 9.5, P = 0.56). It was significantly lower in patients with acanthosis nigricans (AN) as compared with those without AN (8.4 ± 5.9 vs 15 ± 11, P = 0.038). Hirsute patients showed lower mean adiponectin levels than nonhirsute (10 ± 7.3 vs 13 ± 10, P = 0.57). A positive, insignificant correlation was observed between serum adiponectin and cholesterol, low-density lipoprotein, follicle stimulating hormone (FSH), thyroid stimulating hormone, levels. A negative insignificant correlation existed between serum adiponectin and luteinizing hormone (LH), LH: FSH ratio, prolactin, dehydroepiandrosterone, testosterone, triglyceride, high-density lipoprotein, fasting blood glucose, fasting insulin, and Homeostasis Model Assessment. Conclusion: Hypoadiponectinemia is present in one-fifth of women with PCOS. Adiponectin levels decrease as age advances. Low levels of adiponectin possibly contributes to the development of dermal manifestation (AN) of insulin resistance. PMID:24741521

  16. Pediatric non-alcoholic fatty liver disease: Recent solutions, unresolved issues, and future research directions

    Science.gov (United States)

    Clemente, Maria Grazia; Mandato, Claudia; Poeta, Marco; Vajro, Pietro

    2016-01-01

    Non-alcoholic fatty liver disease (NAFLD) in children is becoming a major health concern. A “multiple-hit” pathogenetic model has been suggested to explain the progressive liver damage that occurs among children with NAFLD. In addition to the accumulation of fat in the liver, insulin resistance (IR) and oxidative stress due to genetic/epigenetic background, unfavorable lifestyles, gut microbiota and gut-liver axis dysfunction, and perturbations of trace element homeostasis have been shown to be critical for disease progression and the development of more severe inflammatory and fibrotic stages [non-alcoholic steatohepatitis (NASH)]. Simple clinical and laboratory parameters, such as age, history, anthropometrical data (BMI and waist circumference percentiles), blood pressure, surrogate clinical markers of IR (acanthosis nigricans), abdominal ultrasounds, and serum transaminases, lipids and glucose/insulin profiles, allow a clinician to identify children with obesity and obesity-related conditions, including NAFLD and cardiovascular and metabolic risks. A liver biopsy (the “imperfect” gold standard) is required for a definitive NAFLD/NASH diagnosis, particularly to exclude other treatable conditions or when advanced liver disease is expected on clinical and laboratory grounds and preferably prior to any controlled trial of pharmacological/surgical treatments. However, a biopsy clearly cannot represent a screening procedure. Advancements in diagnostic serum and imaging tools, especially for the non-invasive differentiation between NAFLD and NASH, have shown promising results, e.g., magnetic resonance elastography. Weight loss and physical activity should be the first option of intervention. Effective pharmacological treatments are still under development; however, drugs targeting IR, oxidative stress, proinflammatory pathways, dyslipidemia, gut microbiota and gut liver axis dysfunction are an option for patients who are unable to comply with the recommended

  17. Integrative medicine:the guide line in women's reproductive health and the treatment of gynecological disease

    Institute of Scientific and Technical Information of China (English)

    Yu jin

    2004-01-01

    Integrative medicine follows the principles in TCM that nature and human beings being correspondent with its holistic and dialectic views and explores the integrating point with theories and scientific data in modern medicine. During study on 16 gynecological diseases, the deficiency. In patients with pubertal uterine bleeding, ovulation is induced by acupuncture via decrease in central β-endorphin (β-EP) levels, which leads to the cure of anorexia nervosa by acupuncture via decrease in serum cortisol levels in early 1980s. These results in TCM lead to the idea that neuro-endocrine system is directly related to metabolic system which is also proved to be true in the treatment of polycystic ovary syndrome (PCOS) with or without hyperinsulinemia by two formulas (Yu's Tonifying Ricipe and Tian Gui Recipe,TGR) respectively according to differential diagnosis in TCM. Ovulation resulted with decrease in obesity, acanthosis nigricans, serum androgen, insulin, leptin and hyperthalamic neuropeptide Y (NPY), proopiomalanocortin (POMC) levels based on scientific data from both patients and an animal modal. It suggests that metabolic network, and the incidence of obesity, hypertension, diabetes and cancers of the endometrium and breast may be reduced among patients with PCOS in their future life while these recipes being taken long period. For perimenopausal syndrome, formula Geng Nian Chun (GNC)without increase in serum estrogen levels. In aging rats, it was found that GNC increased the ER and ERmRNA expressions in the ovarian axis and the spleen accompanying with increase in central β-EP, serotonin (5-HT) and peripheral interlutin-2 (IL-2) levels in aging rats without changes of estrogen levels. These data may refer to the release of symptoms in women with postmenopausal syndome. It suggests that GNC up-regulates the fading of neuro-endocrine-immune network. Prevention, alleviation of osteoporosis, cardiovascular disease and Alzheimer disease may be expected in aging

  18. Triglyceride to HDL-C Ratio is Associated with Insulin Resistance in Overweight and Obese Children.

    Science.gov (United States)

    Iwani, Nur Ahmad Kamil Zati; Jalaludin, Muhammad Yazid; Zin, Ruziana Mona Wan Mohd; Fuziah, Md Zain; Hong, Janet Yeow Hua; Abqariyah, Yahya; Mokhtar, Abdul Halim; Wan Nazaimoon, Wan Mohamud

    2017-01-06

    The purpose of this study was to investigate the usefulness of triglyceride to hdl-c ratio (TG:HDL-C) as an insulin resistance (IR) marker for overweight and obese children. A total of 271 blood samples of obese and overweight children aged 9-16 years were analysed for fasting glucose, lipids and insulin. Children were divided into IR and non-insulin resistance, using homeostasis model assessment (HOMA). The children were then stratified by tertiles of TG: HDL-C ratio. The strength between TG:HDL-C ratio and other parameters of IR were quantified using Pearson correlation coefficient (r). Odds ratio was estimated using multiple logistic regression adjusted for age, gender, pubertal stages and IR potential risk factors. Children with IR had significantly higher TG:HDL-C ratio (2.48) (p = 0.01). TG:HDL-C ratio was significantly correlated with HOMA-IR (r = 0.104, p HDL-C ratio showed significant increase in mean insulin level (p = 0.03), HOMA-IR (p = 0.04) and significantly higher number of children with acanthosis nigricans and metabolic syndrome. The odds of having IR was about 2.5 times higher (OR = 2.47; 95% CI 1.23, 4.95; p = 0.01) for those in the highest tertiles of TG:HDL-C ratio. Hence, TG:HDL-C may be a useful tool to identify high risk individuals.

  19. Correlation between serum adiponectin and clinical characteristics, biochemical parameters in Indian women with polycystic ovary syndrome

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    Sunita J Ramanand

    2014-01-01

    Full Text Available Background: Polycystic ovary syndrome (PCOS is a common disorder. PCOS women are at a high risk for insulin resistance and metabolic syndrome (MS. Adiponectin is positively related to insulin sensitivity. It has a preventive role in atherogenesis and MS. The present work was conducted to study the correlation between serum adiponectin levels and clinical characteristics and biochemical parameters in PCOS patients. Materials and Methods: A prospective study in 49 newly diagnosed (as per Rotterdam criteria Indian PCOS women was conducted. PCOS women were clinically examined and investigated for biochemical parameters. Results : The mean serum adiponectin was 12 ± 9.4 μg/mL (range 0.47-45. Hypoadiponectinemia (serum adiponectin <4 μg/mL was present in 22% patients. Age and adiponectin correlated significantly and inversely (r = −0.42, P = 0.027. Overweight/obese patients had lower mean adiponectin levels than normal weight (11.62 ± 9.5 vs 13.58 ± 9.5, P = 0.56. It was significantly lower in patients with acanthosis nigricans (AN as compared with those without AN (8.4 ± 5.9 vs 15 ± 11, P = 0.038. Hirsute patients showed lower mean adiponectin levels than nonhirsute (10 ± 7.3 vs 13 ± 10, P = 0.57. A positive, insignificant correlation was observed between serum adiponectin and cholesterol, low-density lipoprotein, follicle stimulating hormone (FSH, thyroid stimulating hormone, levels. A negative insignificant correlation existed between serum adiponectin and luteinizing hormone (LH, LH: FSH ratio, prolactin, dehydroepiandrosterone, testosterone, triglyceride, high-density lipoprotein, fasting blood glucose, fasting insulin, and Homeostasis Model Assessment. Conclusion: Hypoadiponectinemia is present in one-fifth of women with PCOS. Adiponectin levels decrease as age advances. Low levels of adiponectin possibly contributes to the development of dermal manifestation (AN of insulin resistance.

  20. The skin landscape in diabetes mellitus. Focus on dermocosmetic management

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    Piérard GE

    2013-05-01

    Full Text Available Gérald E Piérard,1 Sophie Seité,2 Trinh Hermanns-Lê,3 Philippe Delvenne,3 André Scheen,4 Claudine Piérard-Franchimont3 1Laboratory of Skin Bioengineering and Imaging (LABIC, University of Liège, Liège, Belgium; 2La Roche-Posay Pharmaceutical Laboratories, Asnières, France; 3Department of Dermatopathology, Unilab Lg, Liège University Hospital, Liège, Belgium; 4Department of Diabetology, Nutrition and Metabolic Diseases, and Clinical Pharmacology Unit, Liège University Hospital, Liège, Belgium Background: Some relationships are established between diabetes mellitus (DM and a series of cutaneous disorders. Specific dermatoses are markers for undiagnosed DM. Other disorders represent supervening complications in an already treated DM patient. Objective: To review the information about dermocosmetic care products and their appropriate use in the management and prevention of dermatoses related to DM. Method: The peer-reviewed literature and empiric findings are covered. Owing to the limited clinical evidence available for the use of dermocosmetics, a review of the routine practices and common therapies in DM-related dermatoses was conducted. Results: Some DM-related dermatoses (acanthosis nigricans, pigmented purpuric dermatosis are markers of macrovascular complications. The same disorders and some others (xerosis, Dupuytren's disease have been found to be more frequently associated with microangiopathy. Other skin diseases (alopecia areata, vitiligo were found to be markers of autoimmunity, particularly in type 1 DM. Unsurprisingly, using dermocosmetics and appropriate skin care has shown objective improvements of some DM-related dermatoses, such effects improve the quality of life. The most common skin manifestations of DM fall along continuum between "dry skin," xerosis, and acquired ichthyosis, occurring predominately on the shins and feet. Dermocosmetic products improve the feeling of well-being for DM patients. Keywords: diabetes

  1. Dermatologic Diseases Presenting with Pigmentation Disorders in Children: A Single Center Experience

    Directory of Open Access Journals (Sweden)

    Ayse Akbas

    2015-12-01

    Full Text Available Background: To determine the incidence and demographic characteristics of skin diseases presenting with hyperpigmentation in children applying to the dermatology department. Methods: A total of 2815 children between the ages of 0 to 16 who applied to the dermatology clinic with the complaints of hyperpigmentation disorders were evaluated. The age, gender, socioeconomic status, place of residence and demographic characteristics of children with abnormal pigmentation skin lesions were investigated. Results: A total of 2815 children applied to the dermatology clinic during the study period. Of these patients 1491 were female (53% and 1324 were male (47%. Of these 266 (9.4% were diagnosed with skin disorders presenting with hyperpigmention. The causes of hyperpigmentation in these patients were was follows; pityriasis alba (2.6%, melanocytic nevus (2.1%, vitiligo (1.8% postinflammatory hyperpigmentation (0.5%, and postinflammatory hypopigmentation (0.5%. According to the gender, p.alba, vitiligo, Becker nevus, acanthosis nigricans, tuberosclerosis and albinism were seen more in boys and nevus depigmentosus ephelis, postinflammatory hyperpigmentation/hypopigmentation and melanocytic nevus were seen more in the girls. Pitriyazis versicolor was seen equally in both genders. According to age groups, melanocytis nevus were found to be more frequent between the ages of 0-2 and 12-16, whereas pityrsasis versicolor was more frequent in ages 12-16 and P. alba in the 3-11 age group. Conclusions: There are quite a substantial number of pigmentation diseases occurring in children. Early diagnosis and treatment are important because although these diseases mainly cause cosmetic problems, they can decrease the quality of life. Here, we attempted to define the demographic characteristics of diseases presenting with disorders in pigmentation in children.

  2. Androgens and women's health.

    Science.gov (United States)

    Redmond, G P

    1998-01-01

    Androgenic disorders are those conditions in women characterized by excessive androgen action. They are the most common endocrinopathy of women, affecting from 10% to 20%. Signs are: persistent acne, hirsutism and androgenic alopecia, which is the female equivalent of male pattern baldness. A subgroup, those traditionally labeled as having polycystic ovary syndrome (PCOS), additionally have anovulation, as well as menstrual abnormalities and, often, obesity. Although women with androgenic disorders usually present themselves for help with the skin or menstrual changes, there are other important implications regarding their health. Women with PCOS have varying degrees of insulin resistance, and an increased incidence of Type II diabetes mellitus, as well as unfavorable lipid patterns. The presence of these risk factors is suggested by upper segment obesity, darkening of the skin, and the other skin changes that make up acanthosis nigricans. Diagnosis involves measurement of circulating androgens (of which free testosterone is most important), together with prolactin and FSH when menstrual dysfunction is present. Many women with androgenic skin changes have normal serum androgen levels, suggesting increased end organ sensitivity to androgens. Others have hyperandrogenism (of ovarian or adrenal origin). Treatment is usually successful in controlling acne, reducing hirsutism and stabilizing, or partially reversing, androgenic alopecia. Pharmacological approaches involve suppressing androgen levels, for example, the use of an appropriate oral contraceptive, or antagonizing androgen action with several medications that have this activity. Unfortunately, most women with androgenic disorders are frustrated in their efforts to obtain medical help. Understanding androgenic disorders will enable the physician to significantly help the majority of women with these conditions.

  3. A clinical study of dermatoses in diabetes to establish its markers

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    Dependra Kumar Timshina

    2012-01-01

    Full Text Available Background: Cutaneous manifestations of diabetes mellitus generally appear subsequent to the development of the disease, but they may be the first presenting signs and in some cases they may precede the primary disease manifestation by many years. Aims : T0 he aim of our study was to study the spectrum of dermatoses in diabetics, to know the frequency of dermatoses specific to diabetes mellitus (DM, and to establish the mucocutaneous markers of DM. Material and Methods: The study was conducted at a diabetic clinic and our department between September 2008 and June 2010. Two hundred and twenty-four diabetic patients were included in the study group and those with gestational diabetes were excluded. Healthy age- and sex-matched individuals were taken as controls. Results: The male to female ratio was 1 : 1.21. Type 2 DM was seen in 89.7% and type 1 DM in 10.3% of the patients. Dermatoses were seen in 88.3% of the diabetics compared to 36% in non-diabetic controls (P<0.05. Cutaneous infections were the most common dermatoses followed by acanthosis nigricans and xerosis in diabetics. Type 2 DM was found to have an increased risk of complications than type 1 DM. Complications of diabetes were seen in 43.7% of the diabetic cases. Diabetic dermopathy, loss of hair over the legs, diabetic foot ulcer, and so on, were found to be the cutaneous markers of DM in our group of cases. Conclusion: Dermatoses were more common in diabetics than non-diabetics. Cutaneous infections formed the largest group of dermatoses in DM.

  4. Bilateral cataracts in a 6-yr-old with new onset diabetes: a novel presentation of a known INS gene mutation.

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    Wasserman, Halley; Hufnagel, Robert B; Miraldi Utz, Virginia; Zhang, Kejian; Valencia, C Alexander; Leslie, Nancy D; Crimmins, Nancy A

    2016-11-01

    The prevalence of diabetes-related cataracts during childhood is less than 1%. When cataracts occur, it is often in adolescent females with prolonged symptoms and significant hyperglycemia. Cataracts are not a classic feature of monogenic diabetes. We report a case of a 6-yr-old, previously healthy Caucasian male, who presented with bilateral acquired cataracts and was subsequently diagnosed with new onset diabetes. Additional symptoms at presentation included a several year history of polyuria and polydipsia, mild hepatomegaly, and short stature. Pertinent negatives include acanthosis nigricans, lipoatrophy, deafness, muscle weakness, or neuropathy. HbA1c was significantly elevated at diagnosis (>14%, 129.5 mmol/mol) without evidence of ketosis. Autoantibody testing was negative. Features of Mauriac syndrome (short stature, hepatomegaly) as well as acquired cataracts indicated long-standing hyperglycemia with sufficient insulin production to prevent ketone production and development of diabetic ketoacidosis. Whole exome sequencing was conducted and a de novo heterozygous mutation in the INS gene (c.94G>A; p.Gly32Ser) was identified. INS gene mutations are common causes of permanent neonatal diabetes but rare causes of antibody-negative diabetes in children. Importantly, INS gene mutations have not been previously associated with acquired cataracts. Knowledge of a monogenic cause of diabetes allows clinicians to tailor counseling and screening of diabetes-related comorbidities. In summary, this case highlights the need to consider testing for monogenic diabetes, specifically INS gene mutations, in pediatric patients with antibody-negative diabetes, especially if complications of prolonged hyperglycemia are present at diagnosis.

  5. Skin Manifestations of Insulin Resistance: From a Biochemical Stance to a Clinical Diagnosis and Management.

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    González-Saldivar, Gloria; Rodríguez-Gutiérrez, René; Ocampo-Candiani, Jorge; González-González, José Gerardo; Gómez-Flores, Minerva

    2017-03-01

    Worldwide, more than 1.9 billion adults are overweight, and around 600 million people suffer from obesity. Similarly, ~382 million individuals live with diabetes, and 40-50% of the global population is labeled at "high risk" (i.e., prediabetes). The impact of these two chronic conditions relies not only on the burden of illnesses per se (i.e., associated increased morbidity and mortality), but also on their increased cost, burden of treatment, and decreased health-related quality of life. For this review a comprehensive search in several databases including PubMed (MEDLINE), Ovid EMBASE, Web of Science, and Scopus was conducted. In both diabetes and obesity, genetic, epigenetic, and environmental factors overlap and are inclusive rather than exclusive. De facto, 70-80% of the patients with obesity and virtually every patient with type 2 diabetes have insulin resistance. Insulin resistance is a well-known pathophysiologic factor in the development of type 2 diabetes, characteristically appearing years before its diagnosis. The gold standard for insulin resistance diagnosis (the euglycemic insulin clamp) is a complex, invasive, costly, and hence unfeasible test to implement in clinical practice. Likewise, laboratory measures and derived indexes [e.g., homeostasis model assessment of insulin resistance (HOMA-IR-)] are indirect, imprecise, and not highly accurate and reproducible tests. However, skin manifestations of insulin resistance (e.g., acrochordons, acanthosis nigricans, androgenetic alopecia, acne, hirsutism) offer a reliable, straightforward, and real-time way to detect insulin resistance. The objective of this review is to aid clinicians in recognizing skin manifestations of insulin resistance. Diagnosing these skin manifestations accurately may cascade positively in the patient's health by triggering an adequate metabolic evaluation, a timely treatment or referral with the ultimate objective of decreasing diabetes and obesity burden, and improving the

  6. The evaluation and management of hirsutism.

    Science.gov (United States)

    Azziz, Ricardo

    2003-05-01

    Hirsutism is the presence of terminal (coarse) hairs in females in a male-like pattern, affecting between 5% and 15% of women, depending on definition. Hirsutism has a significant negative impact on psychosocial development and is usually a sign of an underlying endocrine abnormality-namely, androgen excess. The most common cause of androgen excess is the polycystic ovary syndrome (PCOS), with 21-hydroxylase-deficient nonclassic adrenal hyperplasia, the hyperandrogenic insulin-resistant acanthosis nigricans syndrome, androgen-secreting tumors, and androgenic drug intake occurring less frequently. However, although 70-80% of patients with androgen excess demonstrate hirsutism, this sign may be less prevalent among women of Asian extraction. Conversely, not all hirsute patients have evidence of detectable androgen excess, as 5-15% of these women have "idiopathic hirsutism," with normal ovulatory function and androgen levels. There is a strong familial predilection for hirsutism, primarily because the underlying endocrine disorders (eg, PCOS) and the factors regulating the development of hair growth (eg, androgen receptor activity, 5alpha-reductase activity) have a strong genetic component. The diagnostic evaluation of the potentially hirsute patient first involves confirming the presence of hirsutism and then excluding associated or etiological abnormalities and disorders (eg, ovulatory dysfunction, adrenal hyperplasia, diabetes, thyroid hormone abnormalities). Treatment should be undertaken using combination therapy, to possibly include 1) hormonal suppression (oral contraceptives, long-acting gonadotropin-releasing hormone analogues, and insulin sensitizers), 2) peripheral androgen blockade (spironolactone, flutamide, cyproterone acetate, or finasteride), and 3) mechanical/cosmetic amelioration and destruction of the unwanted hairs (electrology and, potentially, laser hair removal). The application of eflornithine hydrochloride 13.9% topical cream may also be

  7. Pediatric non-alcoholic fatty liver disease: Recent solutions, unresolved issues, and future research directions.

    Science.gov (United States)

    Clemente, Maria Grazia; Mandato, Claudia; Poeta, Marco; Vajro, Pietro

    2016-09-28

    Non-alcoholic fatty liver disease (NAFLD) in children is becoming a major health concern. A "multiple-hit" pathogenetic model has been suggested to explain the progressive liver damage that occurs among children with NAFLD. In addition to the accumulation of fat in the liver, insulin resistance (IR) and oxidative stress due to genetic/epigenetic background, unfavorable lifestyles, gut microbiota and gut-liver axis dysfunction, and perturbations of trace element homeostasis have been shown to be critical for disease progression and the development of more severe inflammatory and fibrotic stages [non-alcoholic steatohepatitis (NASH)]. Simple clinical and laboratory parameters, such as age, history, anthropometrical data (BMI and waist circumference percentiles), blood pressure, surrogate clinical markers of IR (acanthosis nigricans), abdominal ultrasounds, and serum transaminases, lipids and glucose/insulin profiles, allow a clinician to identify children with obesity and obesity-related conditions, including NAFLD and cardiovascular and metabolic risks. A liver biopsy (the "imperfect" gold standard) is required for a definitive NAFLD/NASH diagnosis, particularly to exclude other treatable conditions or when advanced liver disease is expected on clinical and laboratory grounds and preferably prior to any controlled trial of pharmacological/surgical treatments. However, a biopsy clearly cannot represent a screening procedure. Advancements in diagnostic serum and imaging tools, especially for the non-invasive differentiation between NAFLD and NASH, have shown promising results, e.g., magnetic resonance elastography. Weight loss and physical activity should be the first option of intervention. Effective pharmacological treatments are still under development; however, drugs targeting IR, oxidative stress, proinflammatory pathways, dyslipidemia, gut microbiota and gut liver axis dysfunction are an option for patients who are unable to comply with the recommended lifestyle

  8. Skin findings in overweight and obese individuals

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    Hülya Nazik

    2016-06-01

    Full Text Available Background and Design: The aim of the present study is to compare dermatoses detected in overweight and obese individuals with the data obtained from individuals with body mass index (BMI below 25.0 kg/m2 and to emphasize the effects of obesity on skin health. Material and methods: The study was performed with 510 volunteer participants aged above 18 years, who were admitted to a policlinic. One hundred fifty individuals with normal weight who had a BMI below 25.0 kg/m2 constituted the control group, 130 individuals with BMI between 25.0-29.9 kg/m2 constituted the overweight group, and 230 individuals with BMI above 30.0 kg/m2 constituted the obese group. A detailed dermatological examination was performed and the data was recorded. Results: A total of 510 participants, 194 males and 316 females, were included in the study. The mean age was 32.05±10.9, 44.91±13.4, and 39.78±16.4 in the control, overweight, and obese groups, respectively. The most common dermatoses in the overweight and obese groups were striae distensae in 316 individuals (62%, plantar hyperkeratosis in 249 individuals (48.8%, dystrophic cellulitis in 216 individuals (42.4%, acrochordon in 204 individuals (40%, acanthosis nigricans 135 (26.4%, varicose veins in 134 individuals (26.3%, and keratosis pilaris in 108 individuals (21.2%. Conclusion: Several dermatoses are more frequently seen in obese and overweight individuals when compared with normal weight individuals due to insulin resistance and mechanical effects.

  9. Biochemical and immunological investigations on hypothyroidism in dogs.

    Science.gov (United States)

    Gosselin, S J; Capen, C C; Martin, S L; Targowski, S P

    1980-04-01

    Circulating antibody titers (1:20 to 1:2560) against thyroglobulin were demonstrated in 48% of pet dogs with hypothyroidism by the chromic chloride passive hemagglutination test. Four of six dogs with acanthosis nigricans (1:20) and one of six male dogs with hyperestrogenism (1:40) had low titers of antibody against thyroglobulin whereas clinically normal pet dogs and dogs with other selected endocrinopathies (hypoadrenocorticism, cortisol-excess, diabetes mellitus) or obesity were consistently negative. Circulating immune complexes evaluated by the mastocytoma cell-assay were present in the sera of 20% of pet dogs with hypothyroidism but were absent in clinically normal dogs. Although variations in dose significantly altered the quantitative response of the thyroid gland to thyrotropin the qualitative pattern of response was similar for T3 but not T4 in clinically normal laboratory beagles. The peak increases for serum triiodothyronine and thyroxine were observed either at eight (0.1 and 0.2 I.U bTSH/5 lbs) or 12 (1.0 I.U. bTYSH/5 lbs) hours postthyrotropin. Dogs with naturally occurring hypothyroidism had a decreased serum T3 and T4 at baseline and eight hours postthyrotropin (1.0 I.U. bTSH/5 lbs) compared to clinically normal pet dogs, laboratory beagles and dogs with other clinical endocrinopathies. The consistent lack of a significant increase of serum T3 and T4 in response to thyrotropin was necessary for the separation of certain hypothyroid from euthyroid pet dogs in which the baseline level of thyroid hormones were equivocal.

  10. Advances in SAHA syndrome%SAHA综合征研究进展

    Institute of Scientific and Technical Information of China (English)

    张添; 管海宏; 车敦发

    2012-01-01

    SAHA综合征是女性患者雄激素功能过强而引起的一组皮肤症候群,主要临床特征为皮脂溢、痤疮、多毛及雄激素脱发.该综合征可在一系列疾病导致的外周雄激素水平增高基础上发生,也可由毛囊皮脂腺组织对正常循环雄激素水平过于敏感的应答导致.临床分为特发型,卵巢型,肾上腺型,高催乳素型和高雄激素-胰岛素抵抗-黑棘皮型.应在明确病因和临床类型的基础上个体化治疗.%As a group of cutaneous symptoms caused by excessive androgen in women,SAHA syndrome is clinically characterized mainly by seborrhea,acne,hirsutism and androgenetic alopecia.The syndrome occurs either on the basis of high levels of circulating free androgens caused by a series of disorders,or due to the hypersensitive response of pilosebaceous apparaus to normal levels of circulating androgen.Clinically,it is classified into idiopathic,ovarian,adrenal,hyperprolactinemic SAHA and HAIR-AN(hyperandrogenism,insulin resistance,acanthosis nigricans)syndrome.Individualized treatment should be taken on the basis of defined etiology and clinical pattern.

  11. Polycystic ovary syndrome: Is obesity a sine qua non? A clinical, hormonal, and metabolic assessment in relation to body mass index

    Directory of Open Access Journals (Sweden)

    Pikee Saxena

    2012-01-01

    Full Text Available Objective : To determine the proportion of polycystic ovarian syndrome (PCOS patients who have normal body mass index (BMI and to compare the clinical, hormonal, and metabolic profile between lean and overweight patients of PCOS. Materials and Methods: One hundred consecutive infertile women with PCOS were studied and divided into lean (BMI between 18.5 and 23 and overweight (BMI ≥ 23. Metabolic and hormonal profile (serum FSH, LH, testosterone, prolactin, TSH on days 2-3 of menstrual cycle; serum progesterone premenstrually; serum insulin-fasting and 2 hours postglucose, glucose tolerance test, and fasting serum lipid profile was performed along with pelvic sonogropahy; and clinical features, viz. waist hip ratio, hirsutism, acne, acanthosis nigricans, and clitoromegaly were recorded. Results: 42% of the PCOS subjects had normal BMI. Average age, hirsutism (80.9% vs. 89.7%, irregular cycles (92.8% vs. 96.6%, acne (9.5% vs. 15.5%, clitoromegaly (2.3% vs. 3.4%, endometrial thickness >4 mm (9.5% vs. 15.5%, and hormonal profile were similar in the lean and overweight PCOS groups. Family history of diabetes (9.5% vs. 24.1%, abnormal glucose tolerance test (GTT (4.7% vs. 10.3%, deranged lipid profile (14.2% vs. 31%, and 2-hour postprandial insulin levels were higher in the overweight PCOS (P < 0.05. Insulin resistance was observed in 83.3% of lean PCOS but was still lower than 93.1% seen in overweight PCOS (P < 0.05. Conclusion: 42% of the PCOS had normal BMI, but clinical and hormonal profile was similar to PCOS patients with elevated BMI (overweight/obese. However, insulin resistance is observed in 83.3% of lean PCOS. Family history of diabetes, impaired GTT, deranged lipid profile, and insulin resistance were more prevalent in overweight PCOS.

  12. Anormalidades cardiovasculares e metabólicas em pacientes com a síndrome de Berardinelli-Seip Anormalidades cardiovasculares y metabólicas en pacientes con el síndrome de Berardinelli-Seip Cardiometabolic abnormalities in patients with berardinelli-Seip syndrome

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    Antonio Guedes do Rêgo

    2010-01-01

    Full Text Available FUNDAMENTO: A síndrome de Berardinelli-Seip (SBS ou lipodistrofia generalizada congênita acomete, frequentemente, o aparelho cardiovascular e também promove anormalidades metabólicas que envolvem os metabolismos glicídico e lipídico. OBJETIVO: Avaliar a prevalência das anormalidades cardiovasculares e metabólicas em portadores da SBS. MÉTODOS: Vinte e dois pacientes do Estado do Rio Grande do Norte (Brasil, com diagnóstico da SBS, foram submetidos a avaliação clínica, eletrocardiograma de repouso, ecodopplercardiograma, radiografia de tórax, eletrocardiografia dinâmica de 24 horas, teste ergométrico e análise laboratorial. RESULTADOS: Os pacientes eram, predominantemente, adultos jovens, sendo a maioria do sexo feminino. A totalidade da amostra apresentou resistência à insulina, acanthosis nigricans e HDL-colesterol diminuído. A presença de esplenomegalia, hepatomegalia, diabetes mellitus tipo II e triglicérides elevados era constante. A síndrome metabólica foi caracterizada na maioria dos pacientes, com predominância no sexo feminino e com um alto grau de consanguinidade paterna. A hipertensão arterial sistêmica e a pré-hipertensão foram encontradas em mais da metade dos pacientes (77,3%. O ecodopplercardiograma mostrou a presença de hipertrofia concêntrica do ventrículo esquerdo (50%, hipertrofia excêntrica do ventrículo esquerdo (4,5% e geometria normal do ventrículo esquerdo (45,5%. Elevada taxa de arritmia foi evidenciada no holter, tais como extrassístoles ventriculares, extrassístoles supraventriculares e taquicardia supraventricular sustentada. A incompetência cronotrópica (54,5% foi observada no teste ergométrico. CONCLUSÃO: Anormalidades cardiovasculares e metabólicas foram observadas em elevada prevalência em indivíduos jovens e assintomáticos com SBS. Esses achados apontam para a necessidade de acompanhamento cardiológico sistemático e de medidas preventivas nesse grupo de risco

  13. Double diabetes in Saudi Arabia: A new entity or an underestimated condition

    Science.gov (United States)

    Braham, Rim; Alzaid, Aus; Robert, Asirvatham Alwin; Mujammami, Muhammad; Ahmad, Rania Ahmad; Zitouni, Monther; Sobki, Samia Hasan; Al Dawish, Mohamed Abdulaziz

    2016-01-01

    AIM To determine the clinical and biological characteristics of double diabetes (DD) among young people in Saudi Arabia. METHODS This was a retrospective descriptive chart review study including 312 young newly diagnosed diabetic patients (aged 12-20 years), whom were admitted over a five year period (January 2009 to December 2013). Family history of diabetes mellitus (DM) (first degree), physical body mass index (BMI), acanthosis nigricans, history of auto-immune disease and laboratory information for glycosylated hemoglobin, basal C peptide level and diabetes autoantibody response (anti-GAD, anti-IA2 and anti-ICA) were collected from medical report. A mean follow-up of 3 years for these patients was performed. RESULTS Patients were categorized into 4 groups, based on the autoantibody response (Ab+ or Ab-) and C-peptide secretion (β+ for fasting level 0.4-2.1 ng/mL and β- if < 0.4 ng/mL). Group1 (type 1a): Ab+ β- (21%), group 2 (type 1b): Ab- β- (9%), group 3 (DD): Ab+ β+ (31%) and group 4 (classic type 2 DM): Ab- β+ (39%). The mean age of the DD patients in our study was 15.1 ± 6.4 years. A total of 41% of the study population presented with diabetic ketoacidosis and 61% of the study population presented with positive family history of DM. The mean BMI was 26.8 kg/m2 with 64% of overweight or obese patients. Ninety two percent of the patients were started on insulin at the time of diagnosis. During a mean follow-up of 3 years, only 32% of the patients with DD required insulin and 78% were on metformin alone or with insulin. CONCLUSION Our findings enable us to arrive at the conclusion that almost one-third of the young Saudi diabetic patients reveal atypical forms of DM (double diabetes) expressing features resulting from both T1D and T2D. PMID:28031780

  14. 魏子孝“外病内治”皮肤病经验探析%Prof. Wei Zixiao's Experiences in Skin Diseases Based on“Treating the In-ternal for the Disorder on the External”

    Institute of Scientific and Technical Information of China (English)

    邵鑫; 张广德; 魏子孝

    2015-01-01

    Skin disease is the common clinical manifestation or combined disorder in endocrinal dis-eases,manifested as acne,chloasma,diabetic eczema,acanthosis nigricans,skin pruritus,bullous disease, pretibial myxedema,etc. Prof. Wei Zixiao believes that skin disease is the outward manifestation of general disease on the skin and the local skin disorders can induce general disorders. They are interacted and mani-fested each other and caused by the imbalance of yin and yang,and the disturbance of zangfu qi and blood. In the treatment of skin disease,Prof. Wei stresses on“treating the internal for the disorder on the external”. Balancing yin and yang,overall regulation,determining the treating principle by syndrome differentiation, strengthening the antipathogenic qi and eliminating the pathogens are the main thoughts on the treatment of skin disease. And the good efficacy has been achieved.%皮肤病是内分泌疾病常见的临床表现或伴发疾病,临床常见有痤疮、黄褐斑,糖尿病伴发湿疹、黑棘皮病、皮肤瘙痒症、大疱病、胫前黏液水肿等。魏子孝教授认为,皮肤病是人体全身性疾病在皮肤上的表现,同时局部的皮肤病变又可引起全身性病变,二者互为表现又相互影响,是体内阴阳平衡失调、脏腑气血紊乱的表现,对于皮肤病的治疗,魏师强调“外病内治”,其以阴阳平衡、整体调节、辨证论治、扶正祛邪为主要临证思想辨治皮肤病,取得了良好效果。

  15. [Primary lipodystrophies].

    Science.gov (United States)

    Capeau, J; Magré, J; Lascols, O; Caron, M; Béréziat, V; Vigouroux, C

    2007-02-01

    Primary lipodystrophies represent a heterogeneous group of very rare diseases with a prevalence of less than 1 case for 100.000, inherited or acquired, caracterized by a loss of body fat either generalized or localized (lipoatrophy). In some forms, lipoatrophy is associated with a selective hypertrophy of other fat depots. Clinical signs of insulin resistance are often present: acanthosis nigricans, signs of hyperandrogenism. All lipodystrophies are associated with dysmetabolic alterations with insulin resistance, altered glucose tolerance or diabetes and hypertriglyceridemia leading to a risk of acute pancreatitis. Chronic complications are those resulting from diabetes involving the retina, kidney and nerves, cardiovascular complications and steatotic liver lesions that could result in cirrhosis. Genetic forms of generalized lipodystrophy (or Berardinelli-Seip syndrome) result, in most cases, from recessive mutations in one of two genes: either BSCL2 coding seipin or BSCL1 coding AGPAT2, an acyl-transferase involved in triglyceride synthesis. Acquired generalized lipodystrophy (Lawrence syndrome) is of unknown origin but is sometimes associated with signs of autoimmunity. Partial lipodystrophies can be familial with dominant transmission. Heterozygous mutations have been identified in the LMNA gene encoding nuclear lamin A/C belonging to the nuclear lamina, or in PPARG encoding the adipogenic transcription factor PPARgamma. Some less typical lipodystrophies, associated with signs of premature aging, have been linked to mutations in LMNA or in the ZMPSTE24 gene encoding the protease responsible for the maturation of prelamin A into lamin A. Acquired partial lipodystrophy (Barraquer-Simons syndrome) is characterized by cephalothoracic fat loss. Its aetiology is unknown but mutations in LMNB2, encoding the lamina protein lamin B2, could represent susceptibility factors. Highly active antiretroviral treatments for HIV infection are currently the most frequent cause

  16. Prevalence of insulin resistance in obese adolescents

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    Aman B. Pulungan

    2013-01-01

    Full Text Available Background Childhood obesity is a global health problem, with the prevalence is differed in each country and affected by many factors, such as lifestyle and physical activity. Insulin resistance (IR as a basic mechanism of several metabolic diseases in obesity, is related with metabolic syndrome (MetS along with its long term complications, such as type 2 diabetes mellitus (T2DM. Several factors are known to be associated with IR, and the presence of acanthosis nigricans (AN has an important meaning in predicting IR.Objectives To assess the prevalence of IR, MetS in obese adolescents and its potentially associated factors, such as gender, signs of AN, and family history of metabolic diseases.Methods A cross-sectional study was performed in obese adolescents, aged 12-15 years, over a two-month period. Fasting blood glucose, insulin, and lipid profiles were measured. Oobesity was defined using body mass index (BMI. Insulin resistance was quantified by the homeostasis model assessment for IR (HOMA-IR. Metabolic syndrome was defined according to the International Diabetes Federation (IDF 2007 criteria.Results Of 92 obese adolescents, IR was found in 38% of subjects, with females predominating (57.2%. Signs of AN were seen in 71.4% of subjects and a positive family history of metabolic diseases was found in 82.8% of subjects, including family history of obesity, type 2 diabetes mellitus (T2DM, and hypertension. Less than 10% of subjects were considered to be in a prediabetic state, and none had T2DM. No statistical significance was found between gender, family history, or signs of AN and IR (P>0.05. Metabolic syndromes was found in 19.6% of subjects, with the following prevalences for each component: 34.8% for hypertension, 78.3% for central obesity, 8.7% for impaired fasting glucose (IFG, 22.8% for low levels of HDL, and 21.7% for high triglyceride levels. A strong correlation was found between IR and IFG with OR=5.69 (95%CI 1.079 – 29.993, P=0

  17. Prevalence of insulin resistance in obese adolescents

    Directory of Open Access Journals (Sweden)

    Aman B. Pulungan

    2013-05-01

    Full Text Available Background Childhood obesity is a global health problem, with the prevalence is differed in each country and affected by many factors, such as lifestyle and physical activity. Insulin resistance (IR as a basic mechanism of several metabolic diseases in obesity, is related with metabolic syndrome (MetS along with its long term complications, such as type 2 diabetes mellitus (T2DM. Several factors are known to be associated with IR, and the presence of acanthosis nigricans (AN has an important meaning in predicting IR. Objectives To assess the prevalence of IR, MetS in obese adolescents and its potentially associated factors, such as gender, signs of AN, and family history of metabolic diseases. Methods A cross-sectional study was performed in obese adolescents, aged 12-15 years, over a two-month period. Fasting blood glucose, insulin, and lipid profiles were measured. Oobesity was defined using body mass index (BMI. Insulin resistance was quantified by the homeostasis model assessment for IR (HOMA-IR. Metabolic syndrome was defined according to the International Diabetes Federation (IDF 2007 criteria. Results Of 92 obese adolescents, IR was found in 38% of subjects, with females predominating (57.2%. Signs of AN were seen in 71.4% of subjects and a positive family history of metabolic diseases was found in 82.8% of subjects, including family history of obesity, type 2 diabetes mellitus (T2DM, and hypertension. Less than 10% of subjects were considered to be in a prediabetic state, and none had T2DM. No statistical significance was found between gender, family history, or signs of AN and IR (P>0.05. Metabolic syndromes was found in 19.6% of subjects, with the following prevalences for each component: 34.8% for hypertension, 78.3% for central obesity, 8.7% for impaired fasting glucose (IFG, 22.8% for low levels of HDL, and 21.7% for high triglyceride levels. A strong correlation was found between IR and IFG with OR=5.69 (95%CI 1.079 – 29.993, P=0

  18. The evaluation of the impact of age, skin tags, metabolic syndrome, body mass index, and smoking on homocysteine, endothelin-1, high-sensitive C-reactive protein, and on the heart

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    Omar Soliman El Safoury

    2013-01-01

    Full Text Available Background: Skin tags (STs are small, pedunculated skin-colored or brown papules that occur around any site where skin folds occur. The literature is short of comprehensive and controlled clinical studies aimed to evaluate the atherogenic risk factors in patients with STs. Aim of Work: The aim of this study is to evaluate the impact of age, STs, metabolic syndrome (METs, body mass index (BMI, and smoking on homocysteine (Hcy, endothelin-1 (ET-1, high-sensitive C-reactive protein (Hs-CRP, and on cardiovascular diseases. Materials and Methods: This study included 30 cardiac patients with STs, 30 non-cardiac patients with STs, and 30 healthy controls with neither heart disease nor STs. History of smoking, measurement of height, weight, BMI, waist circumference (WC, blood pressure, STs number, color, acanthosis nigricans, estimation of serum level of fasting glucose, triglycerides (TGs, cholesterol, high-dense lipoproteins (HDL, Hcy, ET-1, Hs-CRP, and the presence of the METs were elicited in the three groups. Results: Regarding the Hcy, ET-1, and Hs-CRP, the cardiac-STs group showed the highest levels and the control group showed the least ( P < 0.001. The percents of patients with METs were 56.7% in the cardiac-STs, 40% in the non-cardiac-STs, and 0% in the control group ( P < 0.001. Mean BMI exceeded the limit of obesity in the cardiac-STs group (30.9 ± 3.9 and the non-cardiac-STs group (32.6 ± 6 and was normal in the control group (24.7 ± 2.8. Hyperpigmented STs were present in 66.7% of the cardiac-STs group. Multivariate regression analysis for the independent effectors on Hcy level were the presence of STs ( P < 0.001, METs ( P = 0.001, and BMI ( P = 0.024. Regarding ET-1, the effectors were the presence of STs and METs ( P = 0.032. For Hs-CRP, effectors were the presence of STs ( P < 0.001 and smoking ( P = 0.040. Multivariate logistic regression of the predictors of cardiac disease showed that the independent predictors of the occurrence

  19. Diagnosis and treatment of type 2 diabetes in children and adolescents%儿童及青少年2型糖尿病的诊断和治疗

    Institute of Scientific and Technical Information of China (English)

    赵莎; 马向华; 倪娟

    2013-01-01

    儿童及青少年糖尿病一直是威胁儿童及青少年健康成长的重要危险因素,目前儿童及青少年糖尿病中2型糖尿病占很大一部分.对儿童及青少年2型糖尿病的诊断主要是与1型糖尿病的鉴别.儿童及青少年2型糖尿病多伴有肥胖、多囊卵巢综合征(PCOS)、黑棘皮病等,可以通过临床症状、空腹血糖、口服葡萄糖耐量试验(OGTT)、胰岛素自身抗体和C肽水平来诊断儿童及青少年2型糖尿病.儿童及青少年2型糖尿病与1型糖尿病相比,更早、更易出现糖尿病微血管并发症.目前的治疗方案主要有糖尿病健康教育、饮食控制、运动疗法、口服药物和胰岛素治疗.%Diabetes has been an important risk factor which threats the health of children and adolescents.Type 2 diabetes accounted for a large part in the amount of children and adolescents with diabetes.Diag-nosis of type 2 diabetes in children and adolescents is mainly to identify from type 1 diabetes mellitus.Many children and adolescents with type 2 diabetes usually have problem with obesity,polycystic ovary syndrome (PCOS) or acanthosis nigricans,etc.The diagnosis of children and adolescents with type 2 diabetes can through the clinical symptoms,fasting glucose,oral glucose tolerance test (OGTT),insulin autoantibodies and C-peptide levels.Compared with type 1 diabetes,children and adolescents with type 2 diabetes develop micro-vascular complications more easily and earlier.The treatment of children and adolescents with type 2 diabetes includes diabetic health education,diet control,exercise,oral drugs and insulin.

  20. Insulin resistance in adolescents with Down syndrome: a cross-sectional study

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    Beserra Izabel CR

    2005-06-01

    Full Text Available Abstract Background The prevalence of diabetes mellitus is higher in individuals with Down syndrome (DS than in the general population; it may be due to the high prevalence of obesity presented by many of them. The aim of this study was to evaluate the insulin resistance (IR using the HOMA (Homeostasis Model Assessment method, in DS adolescents, describing it according to the sex, body mass index (BMI and pubertal development. Methods 15 adolescents with DS (8 males and 7 females were studied, aged 10 to 18 years, without history of disease or use of medication that could change the suggested laboratory evaluation. On physical examination, the pubertal signs, acanthosis nigricans (AN, weight and height were evaluated. Fasting plasma glucose and insulin were analysed by the colorimetric method and RIA-kit LINCO, respectively. IR was calculated using the HOMA method. The patients were grouped into obese, overweight and normal, according to their BMI percentiles. The EPIINFO 2004 software was used to calculate the BMI, its percentile and Z score. Results Five patients were adults (Tanner V or presence of menarche, 9 pubertal (Tanner II – IV and 1 prepubertal (Tanner I. No one had AN. Two were obese, 4 overweight and 9 normal. Considering the total number of patients, HOMA was 1.7 ± 1.0, insulin 9.3 ± 4.8 μU/ml and glucose 74.4 ± 14.8 mg/dl. The HOMA values were 2.0 ± 1.0 in females and 1.5 ± 1.0 in males. Considering the nutritional classification, the values of HOMA and insulin were: HOMA: 3.3 ± 0.6, 2.0 ± 1.1 and 1.3 ± 0.6, and insulin: 18.15 ± 1.6 μU/ml, 10.3 ± 3.5 μU/ml and 6.8 ± 2.8 μU/ml, in the obese, overweight and normal groups respectively. Considering puberty, the values of HOMA and insulin were: HOMA: 2.5 ± 1.3, 1.4 ± 0.6 and 0.8 ± 0.0, and insulin: 13.0 ± 5.8 μU/ml, 7.8 ± 2.9 μU/ml and 4.0 ± 0.0 μU/ml, in the adult, pubertal and prepubertal groups respectively. Conclusion The obese and overweight, female and

  1. Type 2 diabetes mellitus in children and adolescents.

    Science.gov (United States)

    Dileepan, Kavitha; Feldt, M Max

    2013-12-01

    On the basis of strong research evidence and consensus, type 1 diabetes mellitus (DM) remains the most common form of DM in children and adolescents. The incidence of type 2 DM in the pediatric population is rapidly increasing because of the obesity epidemic, and minority groups are disproportionately affected. (2) (10) (19) On the basis of some research evidence and consensus, it can be challenging to initially differentiate between type 2 DM and type 1 DM clinically because of the increased prevalence of obesity, the complex interplay of autoimmunity and obesity, and common symptoms at presentation. (1) (10) (19) Significant evidence and consensus support a genetic basis for the development of type 2 DM in children. Physicians should routinely screen at risk children older than age 10 years for DM. Screening criteria include obesity, a family history of type 2 DM, a minority racial or ethnic background, acanthosis nigricans, or other diseases associated with insulin resistance, including polycystic ovary syndrome, hypertension, or dyslipidemia. (1) (10) (18) (19) On the basis of consensus, diagnosis of type 2 DM can be confirmed by an elevated fasting blood glucose level greater than 126 mg/dl (7.0 mmol/L), an elevated 2-hour plasma glucose greater than 200 mg/dL (11.1 mmol/L) on an oral glucose tolerance test, an elevated random blood glucose greater than 200 mg/dL (11.1 mmol/L), or a hemoglobin A1c level greater than 6.5% with suggestive symptoms. (10) According to strong research evidence and consensus, once the diagnosis has been made, treatment should be based on the acuity of presentation and should focus on lifestyle modification and on normalizing hyperglycemia to minimize complications. Metformin is currently first-line treatment for type 2 DM in children and adolescents older than age 10 years who present nonacutely. (18) (19) Strong research evidence and consensus demonstrate that because type 2 DM has an insidious onset, microvascular and

  2. Association of obesity and overweight with the prevalence of insulin resistance, pre-diabetes and clinical–biochemical characteristics among infertile Mexican women with polycystic ovary syndrome: a cross-sectional study

    Science.gov (United States)

    Reyes-Muñoz, Enrique; Ortega-González, Carlos; Martínez-Cruz, Nayeli; Arce-Sánchez, Lidia; Estrada-Gutierrez, Guadalupe; Moran, Carlos; Sánchez-Serrano, Ana Paola; Higareda-Sánchez, Rodolfo; de la Jara-Díaz, Julio Francisco

    2016-01-01

    Objective To study the association of obesity and overweight with the prevalence of insulin resistance (IR), pre-diabetes and clinical–biochemical characteristics among infertile Mexican women with polycystic ovary syndrome (PCOS). Design Retrospective cross-sectional study. Setting Level-three medical institution, an infertility clinic in Mexico City. Participants We included infertile Mexican women with diagnosis of PCOS according to the Rotterdam criteria: group 1 (n=83), normal weight (body mass index (BMI) 18.5–24.9 kg/m2); group 2 (n=217), overweight (BMI 25–29.9 kg/m2); and group 3 (n=238), obese (BMI≥30 kg/m2). Primary and secondary outcome measures IR was determined by homeostatic model assessment (HOMA) >2.5 and pre-diabetes by fasting glucose between 5.6 and 6.9 mmol/L and/or glucose value between 7.8 and 11 mmol/L at 2 hours during an oral glucose tolerance test. We compared clinical–biochemical characteristics among groups. Results Prevalence of IR for groups 1, 2 and 3 was 19.3%, 56.2% and 78.2%; overweight and obesity increase the IR OR (CI 95%) to 5.3 (2.9 to 9.8) and 14.9 (8.0 to 28), respectively. Prevalence of pre-diabetes for groups 1, 2 and 3 was 7.2%, 17.5% and 31.5%; overweight and obesity increase the pre-diabetes OR (CI 95%) to 2.7 (1.1 to 6.7) and 5.9 (2.4 to 14), respectively. Acanthosis nigricans was more frequent in group 3 than group 1. Free Androgen Index (FAI) and thyroid-stimulating hormone (TSH) levels were lower in group 1 than in groups 2 and 3. Progesterone and sex hormone-binding globulin (SHBG) levels were higher in group 1 than in groups 2 and 3. Dehydroepiandrosterone sulfate (DHEA-S) was higher in group 1 than group 3. Conclusions Obese and overweight infertile Mexican women with PCOS, attending to an infertility clinic, have a higher prevalence of IR and pre-diabetes compared with normal-weight women with PCOS. Therapeutic interventions should include those that improved metabolic functioning prior to

  3. Study on the facial and body terminal hair growth in women in Guangdong by using modified Ferriman-Gallwey scoring system%改良Ferriman-Gallwey评分系统用于广东省育龄期汉族妇女体毛评价的分析

    Institute of Scientific and Technical Information of China (English)

    赵晓苗; 倪仁敏; 黄佳; 黄丽丽; 杜尚明; 马梦君; 林淡钰; 杨冬梓

    2013-01-01

    (12/293),正常毛发妇女分别为25.3%(681/2695)、16.2%(437/2695)、1.9% (51/2695),两者分别比较,差异均有统计学意义(P<0.05).结论 (1) mFG评分系统评价广东省育龄期汉族妇女的体毛生长程度随着年龄增加而降低.(2)mFG≥5分为多毛症诊断界值.(3)多毛症妇女痤疮、月经紊乱及多囊卵巢发生率明显升高.%Objective To study growth of facial and body terminal hair of women in Guangdong province and its relationship with age,menstrual irregularities and polycystic ovary,and determine normative cut-off score of modified Ferriman and Gallwey (mFG).Methods A cross-sectional study was conducted on 2988 women at age of 20-45 years from 16 communities of two urban and two rural regions in Guangdong province from June 2008 to July 2009.Terminal body hair growth was assessed by using the modified Ferriman and Gallwey (mFG) scoring system.The normative cut-off value of mFG were calculated by using the K-means cluster analysis (K =2).Those women were classified into following groups,including 982 women at group of ages of 20-years,765 women at group of 26-years,597 women at group of 31-years,384 women at group of 36-years,260 women at group of 41-45 years.Due to absence or errors of medical records,some cases were excluded from this study.Based on menses irregularities (MI),polycystic ovaries (PCO),there were 488 cases in MI group,2413 cases in normal menses group,568 cases in PCO group,and 2207 cases in non-PCO group finally.The incidences of acne,MI,acanthosis nigricans,and polycystic ovaries were also analyzed in all the hirsute groups.Results (1) among 2988 women,it was observed 149women (5%) with mFG ≥7,314 women (10.5%) with ≥ 5,747 women with mFG≥2.(2) Cluster analysis identified an mFG score of 5 as the cut-off value that define abnormal hirsute in the total population and all the sub-groups with/without MI or PCO; (3) Based on age classification,it was found that increased age was associated

  4. A new species of Myotis (Chiroptera: Vespertilionidae) from Suriname

    Science.gov (United States)

    Moratelli, Ricardo; Wilson, Don E.; Gardner, Alfred; Fisher, Robert D.; Gutiérrez, Eliécer E.

    2016-01-01

    We describe a new species of bat in the genus Myotis (Vespertilionidae: Myotinae) from the district of Sipaliwini, Suriname. The new species (Myotis clydejonesi sp. nov.), known from a single specimen, is sister to a clade of M. nigricans (Schinz) from southern South America, but differs from all Neotropical species of Myotis in qualitative and quantitative morphological characters and in its cytochrome-b gene sequence. Our findings also indicate that M. nigricans remains composite and provide support for restricting M. nigricans (sensu stricto) to southern South America.

  5. 儿童青少年糖尿病临床特征及初步分型探讨%Clinical characteristics and primary classification for diabetes in children and adolescents

    Institute of Scientific and Technical Information of China (English)

    范元硕; 罗建华; 于瑞萍; 刘波; 龙本丹

    2013-01-01

    Objective To investigate the clinical characteristics and primary classification of diabetes in children and adolescents. Methods According to fasting C-peptide level, 36 children and adolescents with diabetes were divided into two groups: decreased C-peptide group (fasting C-peptide level<0.60 μg/L, n=24) and normal C-peptide group (fasting C-peptide level≥0.60 μg/L, n=12). Clinical characteristics were compared between two groups. Results Compared with decreased C-peptide group, normal C-peptide group had significantly higher proportion of acanthosis nigricans, family history of type 2 diabetes, metabolic syndrome and overweight/obesity (P< 0.01 or P<0.05). The levels of body mass index, waist circumference, fasting C-peptide, postprandial C-peptide and A C-peptide in normal C-peptide group were significantly higher than those in decreased C-peptide group (all P<0.01). The rate of positive glutamic acid decarboxylase antibody/protein tyrosine phosphate antibody, concurrent ketosis/ke-toacidosis and underweight in normal C-peptide group was significantly lower than that in decreased C-peptide group. (P<0.01 or P<0.05) Normal C-peptide group showed significantly lower levels in random blood glucose and β hy-droxybutyric acid than those in decreased C-peptide group (P<0.01 or P<0.05). Conclusion According to fasting C-peptide level, children and adolescents with diabetes can be primary classified. Decreased C-peptide group is more likely to be classified as type 1 diabetes, and normal C-peptide group is more likely to be classified as type 2 diabetes.%目的 探讨儿童青少年糖尿病患者的临床特征和初步分型.方法 根据空腹C肽水平将36例儿童青少年糖尿病患者分为C肽降低组(空腹C肽<0.60 μg/L,24例)和C肽正常组(空腹C肽≥0.60 μg/L,12例),比较两组患者的临床资料.结果 C肽正常组黑棘皮症、2型糖尿病家族史、代谢综合征及超重/肥胖者的比例明显高于C肽降低组(P<0.01

  6. Cutaneous manifestations of internal malignancies in a tertiary health care hospital of a developing country Manifestações cutâneas de doenças malignas em um hospital terciário de um país em desenvolvimento

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    Alex G Ortega-Loayza

    2010-10-01

    Full Text Available In a public hospital in Lima, Peru, 24 patients with 16 types of paraneoplastic dermatoses were identified by data collection. The most frequent dermatosis was dermatomyositis (four patients. The other dermatoses were malignant acanthosis nigricans, palmoplantar keratoderma, bullous dermatoses, lymphomatoid papulosis, edematous scarring vasculitic panniculitis, Norwegian scabies, primary systemic amyloidosis, necrolytic migratory erythema, infective dermatitis, pancreatic panniculitis, generalized pruritus, Lesser-Trelat syndrome, and acquired ichthyosis. Most of these paraneoplastic dermatoses were diagnosed before (45.8% or at the time of (38.5% the diagnosis of the underlying malignancy. The most frequent underlying malignancies were lymphoma, adenocarcinomas of the upper digestive tract, and malignant neoplasms of the pancreas. The average age of the patients was 47.0 ± 16.9 years and the length of the disease since diagnosis was 13.7 months. The mortality rate was 75%. Paraneoplastic dermatoses are rare dermatologic entities that are difficult to diagnose. Surveillance is also hampered when patients do not have easy access to health care centers due to financial and geographical issues. However, when identified, they might facilitate the early diagnosis of an associated tumor and contribute to increase the surveillance of patients.Em um hospital público em Lima, Peru, 24 pacientes com 16 tipos de dermatoses paraneoplásicas foram identificados por meio de coleta de dados. A dermatose mais frequente foi dermatomiosite (quatro pacientes. As outras dermatoses foram acantose maligna, queratodermia palmoplantar, dermatoses bolhosas, papulose linfomatóide, cicatriz edematosa, paniculite e vasculite, escabiose norueguesa, amiloidose sistêmica primária, eritema necrolítico migratório, dermatite infecciosa, paniculite pancreática, prurido generalizado, sinal de Leser-Trelat e ictiose adquirida. Grande parte dessas dermatoses foi

  7. Investigation of glucose metabolism and obesity in school-age children in Zhengzhou city%郑州地区学龄儿童肥胖特征及糖代谢的调查分析

    Institute of Scientific and Technical Information of China (English)

    袁慧娟; 赵志刚; 汪艳芳; 李德霞; 苏永; 张会峰; 王遂军; 田睿

    2012-01-01

    Objective To investigate the status of children adiposity at school age, and to analysis their related factors. Methods We advertised through the press, and recruited obese children aged 7~14y to our hospital- All the children meeting the diagnostic criteria for obesity filled out the unifiable form and had a physical examinations and laboratory tests. Results The prevalence of diabetes, impaired glucose regulation, insulin resistance,benign acanthosis nigricans and hypertension were 2. 4% ,20. 3% ,43.1 % ,80. 1%,35. 9% respectively among adolescents with adiposity in Zhengzhou city. By multiple linear stepwise regression analysis, we found that birth weight and waist circumference were related to body mass index(P<0. 01), Meanwhile sex,age,waist, fasting plasma glucose were related to fasting serum insulin level (P<0.01). Conclusions The prevalence of glucose dysregulation, insulin resistance and hypertension performance among obese children were increased in Henan province. The degree of obesity in children tended to increase with increasing age gradually. Strengthened screening and management of obese children are very helpful to prevent the high incidence of metabolic disease after being adults .%目的 了解郑州地区学龄儿童肥胖症的患病情况并分析相关影响因素. 方法 通过报纸招募7~14岁肥胖儿童来我院检查,对于符合肥胖症标准的422例儿童填写统一设计的表格并进行体检和实验室检查. 结果 郑州地区学龄肥胖症的儿童,糖尿病、IGR、IR、良性黑棘皮病、高血压的患病率分别为2.4%、20.3%、43.1%、80.1%、35.9%.多元线性逐步回归分析发现,出生时体重和WC与BMI相关(P<0.01),性别、年龄、WC、FPG与FIns水平相关(P<0.01). 结论 郑州地区肥胖儿童的糖调节障碍、IR、高血压的患病率高.儿童的肥胖程度呈现随年龄增加逐渐加重趋势.加强对肥胖儿童的筛查与管理,对防止成年后代谢疾病的高发具有重要意义.

  8. Research progress of polycystic ovary syndrome in adolescents%青春期多囊卵巢综合征的研究进展

    Institute of Scientific and Technical Information of China (English)

    王婷; 柏海燕; 师娟子

    2014-01-01

    多囊卵巢综合征( PCOS)是育龄期女性常见的内分泌疾病,主要表现为月经稀发、痤疮、多毛、肥胖、黑棘皮症等,其一旦确诊意味着远期发生不孕、子宫内膜增生/肿瘤、代谢综合征、2型糖尿病和心血管疾病的风险增高。 PCOS的发病从青春期开始,严重危害着青春期女性身体健康的同时也给其造成了一定的心理问题。青春期PCOS的发病机制中,胰岛素抵抗及高雄激素起至关重要的作用,青春期PCOS的诊断标准在临床上已具有较大的认可度,但仍需要进一步证实,治疗干预则首先从调整生活方式做起,辅以药物治疗,而患病人群心理干预的重要性也逐渐为研究者所重视。该文主要针对青春期PCOS的最新研究成果展开讨论,从发病机制的研究进展、诊断标准的变迁、目前的治疗策略进行逐项分析。%Polycystic ovary syndrome (PCOS) is the most common endocrine disorder for reproductive-age women.It is characterized by menstrual dysfunction, acne, hirsutism, obesity and acanthosis nigricans.Once it is diagnosed, the risk will increase in the following aspects such as infertility , endometrial hyperplasia/carcinoma, metabolic syndrome , type 2 diabetes and cardiovascular disease in far future.PCOS symptoms often start from adolescence .It is harmful to the adolescent physical health as well as mental health .Insulin resistance and hyperandrogenemia play key roles in the pathogenesis of adolescent PCOS .The diagnostic criteria of the adolescent PCOS have been accepted widely in clinic practice , but the criteria still need to be confirmed .The first step of the treatment is the change of lifestyle combined with drug treatment , and the importance of psychotherapy gradually attracts the attention of researchers .This article mainly summarized and discussed the latest researches of adolenscent PCOS on the progress in pathogenesis , changes of diagnostic

  9. Crianças e adolescentes obesos: dois anos de acompanhamento interdisciplinar Obese children and adolescents: two years of interdisciplinary follow-up

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    Mariana Porto Zambon

    2008-06-01

    at mean age of 4.3 years when they started the weight gain. All patients were followed at the Obesity Out-Patient Clinic of the Clinical Hospital of Campinas University. Out of the 150 patients, 128 returned once and weight, height, body mass index (BMI measurements and Z scores were obtained. Laboratory exams were colleted: hemoglobin, lipids, fasting glucose and fasting insulin. Glucose/insulin ratio was calculated. Patients were divided in two groups, if they returned or not to the clinic. Difference between both groups were analyzed regarding age, gender, city of origin, age when the weight gain started, acanthosis nigricans, weight, height, BMI Z score and proportion of altered laboratory exams. The difference in BMI Z score in relation to the first visit was also analyzed. RESULTS: Among the 128 patients, 57% continued to be followed in the clinic and 114 returned at least once. Comparing groups, patients from Campinas fail more to return. There were no differences in the clinical, anthropometric and laboratory variables. Both groups showed lower mean BMI Z score in the return visit compared to the first visit. CONCLUSIONS: The obese children and adolescents from the follow-up clinic showed a reduction in the mean BMI Z score similar to published data. The rate of dropping follow-up was high, but no variable could explain it, except for the patient origin.

  10. Bite force and feeding kinematics in the eastern North Pacific Kyphosidae.

    Science.gov (United States)

    Moran, Clinton Joseph; Ferry, Lara

    2014-04-01

    Some fishes that feed on attached food items possess an intramandibular joint (IMJ), which is thought to increase maximum gape and facilitate contact between the tooth-bearing surface and the substrate. However, the mechanical consequences of using an IMJ to remove attached food items from the substrate are still poorly understood. We examined the most prominent eastern North Pacific kyphosid, the scraper: Girella nigricans and two other kyphosids, Medialuna californiensis and Hermosilla azurea, which occupy similar habitats. Of the three species, G. nigricans had the highest theoretical bite force per unit length. We examined the feeding mechanics of G. nigricans in two different feeding scenarios: a scraping behavior elicited on a block of brine shrimp gelatin and a picking behavior elicited on Ulva sp. We measured cranial elevation, lower jaw rotation, premaxillary protrusion, premaxillary rotation, gape maximum, and intramandibular rotation. Ulva treatments produced significantly greater cranial rotation, when compared to gelatin treatments. Gelatin treatments were associated with greater lower jaw rotation and larger gape. Premaxillary rotation and premaxillary protrusion did not differ between treatments. Intramandibular rotation occurred only when G. nigricans physically contacted the gelatin, suggesting the IMJ is a passive joint with no associated musculature. We also noted that G. nigricans do not appear to use suction to draw food into the mouth. The lack of suction and the presence of the IMJ suggest that the jaws of G. nigricans are specialized for maximizing jaw force when scraping.

  11. Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias

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    Fernanda Visioli

    2012-06-01

    Full Text Available OBJECTIVES: The aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias. MATERIAL AND METHODS: This was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias. excisional biopsies were performed and the patients remain under clinical follow-up. The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias. Paraffin-embebeded sections were immunostained for p53 and p21WAF1. Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells. A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers. Groups were compared with ANOVA (p0.05. CONCLUSIONS: Our findings failed to differentiate the non-dysplastic lesions by means of p53 and p21WAF1 immunostaining, notwithstanding similar profiles between non-dysplastic and dysplastic leukoplakias were observed.

  12. Environmental and Health Effects Review for Obscurant Fog Oil

    Science.gov (United States)

    1993-09-01

    clothing) can break down the protective defences of the skin barrier, causing inflammation, acanthosis, and eczema (Liss-Suter et al. 1978a; Smith et...34Pulmonary Pathology from Inhalation of a Complex Mineral Oil Mist in Dogs , Rats, Mice and Gerbils." Amer. Ind. Hyg. Assoc. J. 39:393-399. Sullivan, J. D

  13. 青春期多囊卵巢综合征患者临床、内分泌代谢紊乱特征及诊断初探%Clinical features, reproductive endocrine and metabolic abnormalities of adolescent girls with polycystic ovary syndrome

    Institute of Scientific and Technical Information of China (English)

    赵赟; 徐晶晶; 朱铭伟; 林金芳

    2010-01-01

    Objective To investigate the clinical features,reproductive endocrine and metabolic abnormalities in adolescent girls with polycystic ovary syndrome (PCOS).Methods A total of 325 adolescent girls with normal menstruation,18 obese (OB-CON) and 307 non-abese (NOB-CON),were enrolled as controls from multiple middle schools in Shanghai,China.A total of 167 adolescent girls with PCOS,90 obese (OB-PCOS) and 77 non-obese (NOB-PCOS),were also recruited.All cases were evaluated for their clinical manifestations, reproductive endocrine and metabolic parameters.Hyperandrogenism was determined by serum testosterone (T),free androgen index (FAI) and dehydroepiandrosterone sulfate (DHEA-S).Insulin sensitivity was measured by fasting insulin (FINS) and homeostasis model assessment of insulin resistance (HOMA-IR).Results (1) Menarche Was significantly earlier in adolescent PCOS than in controls.The incidence of obesity Was significantly higher in adolescent PCOS than that in control group.(2) T,FAI and DHEA-S were significantly higher in adolescent PCOS group than those in control group.FAI Was higher in OB-PCOS group than in NOB-PCOS group.LH and LH/FSH were higher in PCOS groups than those in controls.LH and LH/FSH were also much higher in NOB-PCOS group than those in OB-PCOS group.(3) HOMA-IR and FINS were significantly higher in PCOS group than those in control group. Incidence of acanthosis nigricans,FINS,HOMA-IR and triglyceride were significantly higher in OB-PCOS group than those in NOB-PCOS group.(4) 95.21% PCOS girls presented with an ultrasonic morphological evidence of polycystic ovarian.Conclusion The essential features of adolescent PCOS are an earlier onset of menarche,a persistent menstrual disorder over 2 years after menarche,a higher incidence of obesity,marked hyperandrogenism and insulin resistance and disorderly gonadotropine secretion in comparison with control subjects.Hyperandrogenism and insulin resistance are much more severe in obese adolescent PCOS

  14. Prevalência de alterações metabólicas em crianças e adolescentes com sobrepeso e obesidade: uma revisão sistemática Prevalence of metabolic changes in children and adolescents: a systematic review

    Directory of Open Access Journals (Sweden)

    Lúcia Gomes Rodrigues

    2011-06-01

    Health database. The inclusion criteria were: metabolic syndrome prevalence data in children and adolescents with overweight and obes-ity, and publications in Portuguese, English, Spanish and French. The exclusion criteria were review articles and short communications, investigations enrolling participants with genetic, endocrine and immunologic diseases, primary hy-pertension, and acanthosis nigricans. DATA SYNTHESIS: The review afforded 1.226 abstracts, being 65 selected to be read, 46 of them matched the afore-mentioned selection criteria and could be retrieved. They were published between 2003 and 2009 and represented five geographic regions: North America (33%, South America (20%, Central America (4%, Asia (30% and Europe (13%. The metabolic syndrome reported prevalence ranged from 2.1 to 58.3%. The adopted criteria diverged among the studies, 26 of them used the same components (neutral fat, HDL, glucose, waist circumference and blood pressure, with a median prevalence of 31.2%, without agreement on the chosen cut-off points. In the remaining studies, metabolic syndrome definition included glucose oral tolerance, body mass index, serum cholesterol, and HOMA-IR index. CONCLUSIONS: The metabolic syndrome prevalence among children and adolescents with obesity or overweight reported in the literature showed a wide variability. There was heterogeneity, regarding born the variables chosen to define the presence of metabolic syndrome and their respective cut-off points.

  15. Mosquito studies on the Isla de la Juventud, Cuba, at the beginning of rain period.

    Science.gov (United States)

    Ryba, J; Fuentes, O; Danielová, V; Fernández, A

    1984-01-01

    A total of 8418 mosquitoes belonging to 16 species were collected in 11 localities of the Isla de la Juventud. The most abundant species was Aedes taeniorhynchus (92% of specimens collected). The species Wyeomyia vanduzeei and Mansonia nigricans were encountered on the island for the first time.

  16. Ice break-up and snow-melt as spring indices of reproductive effort and population size of Pacific brant at the Kashunuk River, Yukon-Kuskokwim Delta, Alaska

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Yukon-Kuskokwim Delta (YKD) is the most southerly (60° to 62°N) and the only sub-Arctic breeding area of Pacific brant (Branta bemicla nigricans) supporting an...

  17. Searching chromosomal landmarks in Indian lentils through EMA-based Giemsa staining method.

    Science.gov (United States)

    Jha, Timir Baran; Halder, Mihir

    2016-09-01

    Lentil is one of the oldest protein-rich food crop with only one cultivated and six wild species. India is one important cultivator, producer and consumer of lentils and possesses a large number of germplasms. All species of lentil show 2n = 14 chromosomes. The primary objective of the present paper is to search chromosomal landmarks through enzymatic maceration and air drying (EMA)-based Giemsa staining method in five Indian lentil species not reported elsewhere at a time. Additionally, gametic chromosome analysis, tendril formation and seed morphology have been studied to ascertain interspecific relationships in lentils. Chromosome analysis in Lens culinaris, Lens orientalis and Lens odemensis revealed that they contain intercalary sat chromosome and similar karyotypic formula, while Lens nigricans and Lens lamottei showed presence of terminal sat chromosomes not reported earlier. This distinct morphological feature in L. nigricans and L. lamottei may be considered as chromosomal landmark. Meiotic analysis showed n = 7 bivalents in L. culinaris, L. nigricans and L. lamottei. No tendril formation was observed in L. culinaris, L. orientalis and L. odemensis while L. nigricans and L. lamottei developed very prominent tendrils. Based on chromosomal analysis, tendril formation and seed morphology, the five lentil species can be separated into two distinct groups. The outcome of this research may enrich conventional and biotechnological breeding programmes in lentil and may facilitate an easy and alternative method for identification of interspecific hybrids.

  18. Odonatos asociados al curso superior y medio del río Suquía, Córdoba, Argentina

    Directory of Open Access Journals (Sweden)

    Adriana I. ZAPATA

    2016-01-01

    Full Text Available Se presenta la primera lista de la odonatofauna regional de Córdoba con 24 especies. Acanthagrion lancea Selys, Telebasis willinki Fraser (Zygopte- ra: Coenagrionidae, Erythrodiplax nigricans (Rambur, E. umbrata (Linnaeus, Mi- athyria marcella (Selys in Sagra, Orthemis nodiplaga Karsch, Pantala flavencens (Fabricius y P. hymenaea (Say (Anisoptera: Libellulidae constituyen primeros re- gistros de la provincia.

  19. Diet of two sympatric insectivores bats (Chiroptera: Vespertilionidae in the Cerrado of Central Brazil Dieta de duas espécies simpátricas de morcegos insetívoros no Cerrado do Brasil Central

    Directory of Open Access Journals (Sweden)

    Ludmilla M. S. Aguiar

    2008-03-01

    Full Text Available We examined food habits of Vespertilionidae bats Myotis nigricans (Schinz, 1821 and Eptesicus furinalis (d'Orbigny, 1847 by fecal analysis in cerrado sensu stricto and gallery forests, within APA - Gama-Cabeça-de-Veado, Brasília, Distrito Federal, Brazil. Out of 20 fecal samples collected, seven were of Eptesicus furinalis and 13 of Myotis nigricans. The diet of E. furinalis included six orders of insects: Coleoptera (5/7 by items presence, Lepidoptera and Hymenoptera (3/7, Diptera, Hemiptera and Homoptera (1/7. The diet of M. nigricans included all the main orders consumed by E. furinalis (6/13, 4/13, 4/13, 3/13, 1/13, and 4/13 respectively and one other order: Orthoptera (1/13. Homoptera, Diptera and Orthoptera were collected only in bats captured in gallery forest. There is 80% of overlap in the diet of these two species. Predation on species of Scarabeidae, Hesperiidae, Sphingidae and Saturniidae families confirms bats potential as biological control agents of pests in agricultural ecosystems.Foi examinado o hábito alimentar das espécies de Vespertilionidae Myotis nigricans (Schinz, 1821 e Eptesicus furinalis (d'Orbigny, 1847 por meio de análise de amostras fecais coletadas em animais capturados em área de cerrado sensu stricto e matas de galeria, na APA - Gama-Cabeça-de-Veado, Brasília, Distrito Federal, Brasil. Um total de 20 amostras fecais foi analisado, sendo sete de E. furinalis e 13 de M. nigricans. A dieta de E. furinalis incluiu seis ordens de insetos: Coleoptera (5/7 (presença na amostra total, Lepidoptera e Hymenoptera (3/7, Diptera, Hemiptera e Homoptera (1/7. A dieta de M. nigricans incluiu todas as ordens consumidas por E. furinalis (6/13, 4/13, 4/13, 3/13, 1/13, and 4/13 respectively e uma ordem a mais: Orthoptera (1/13. Homoptera, Diptera e Orthoptera só foram amostrados para morcegos capturados em mata de galeria. Há 80% de sobreposição na dieta destas duas espécies. A predação sobre espécies das fam

  20. Angiokeratoma of fordyce in a children

    Directory of Open Access Journals (Sweden)

    Ömer Çalka

    2014-12-01

    Full Text Available Angiokeratomas are benign tumors characterized by epidermal hyperkeratosis, acanthosis and multiple dilated blood vessels in the papillary dermis. Angiokeratoma of Fordyce is one of five types in the group of the angiokeratomas, which occurs on the scrotum, penis or vulva. It is usually observed in young adults or elderly men. A 6-year-old boy presented to the dermatology department because of papular and erythematous lesions on his scrotum and penis. These lesions were found at birth and were asymptomatic. There was a history of occasional bleeding on trauma from the lesions. Histological evaluation of a skin biopsy specimen showed hyperkeratosis and acanthosis of the epidermis and multiple dilated thin-walled vessels in the papillary dermis. Based on the clinical, histopathological and dermoscopic findings, the patient was diagnosed with Fordyce angiokeratoma. Herein, we report a case of angiokeratomas of Fordyce, which is very rare in childhood and the dermoscopic findings

  1. Dermatophilosis (Cutaneous streptothricosis) in Kafue lechwe (Kobus leche kafuensis).

    Science.gov (United States)

    Pandey, G S; Mweene, A; Suzuki, A K; Nambota, A; Kaji, T

    1994-10-01

    Extensive dermatitis caused by Dermatophilus congolensis was identified in two kafue lechwe (Kobus leche kafuensis) in Lochinvar National Park of Zambia. The lesions were characterized by thickening of the skin, crusts, and nodfule formation. Almost all parts of the body were affected. Histologically there was an exudative dermatitis with acanthosis, parakeratosis, hyperkeratosis, and an exudate rich in neutrophils. This is the first known report of dermatophilosis in lechwe.

  2. Study on clinical characteristics of metabolic-based obesity classification%基于代谢分类的不同肥胖表型的临床特点分析

    Institute of Scientific and Technical Information of China (English)

    王兴纯; 黄玥晔; 孙航; 曹涵; 陈佳奇; 卜乐; 曲伸

    2015-01-01

    97.14) μmol/L,bothP<0. 05]. Patients in IMO group had acanthosis nigricans, but their glucose level was relatively normal. Conclusion The metabolic-based obese diagnosis is essential for understanding the obesity etiology and providing individualized treatment.

  3. Mixed culture optimization for marigold flower ensilage via experimental design and response surface methodology.

    Science.gov (United States)

    Navarrete-Bolaños, José Luis; Jiménez-Islas, Hugo; Botello-Alvarez, Enrique; Rico-Martínez, Ramiro

    2003-04-09

    Endogenous microorganisms isolated from the marigold flower (Tagetes erecta) were studied to understand the events taking place during its ensilage. Studies of the cellulase enzymatic activity and the ensilage process were undertaken. In both studies, the use of approximate second-order models and multiple lineal regression, within the context of an experimental mixture design using the response surface methodology as optimization strategy, determined that the microorganisms Flavobacterium IIb, Acinetobacter anitratus, and Rhizopus nigricans are the most significant in marigold flower ensilage and exhibit high cellulase activity. A mixed culture comprised of 9.8% Flavobacterium IIb, 41% A. anitratus, and 49.2% R. nigricans used during ensilage resulted in an increased yield of total xanthophylls extracted of 24.94 g/kg of dry weight compared with 12.92 for the uninoculated control ensilage.

  4. Examination of mycological samples by means of the scanning electron microscope

    Directory of Open Access Journals (Sweden)

    M. Thibaut

    1973-04-01

    Full Text Available Three species of Siphomycetes: Rhizopus arhizus, Rhizopus equinus and Rhizopus nigricans, as well as a Septomycete: Emericella nidulans, have been examined by means of a scanning electron microscope. Among the difjerent Rhizopus, this technique showed differences in the appearance of the sporangia. In Emericella nidulans, scanning microscopy enábled one to ascertain that the "Hull cells" were completely hollow and also demonstrated the ornemented aspect of the ascospores.Três espécies de Sifomicetas: Rhizopus arhizus, Rhizopus equinus, Rhizopus nigricans e um Septomiceta: Emericella nidulans foram examinados em microscopia de exploração. Esta técnica mostrou detalhes não evidenciáveis ao poder de resolução do microscópio óptico, demonstrando ser útil para o diagnóstico em micologia.

  5. Pedo-ecological conditions of rare plant species in Romania. Tofieldia calyculata L. Wahlbg.

    Directory of Open Access Journals (Sweden)

    Vasile MICLAUS

    1979-08-01

    Full Text Available The paper present data's on the pedologycal conditions which preserved the endangered species Tofieldia calyculata L. Wahlbg. on a restricted territory in Valea Morii, near Cluj-Napoca, Transylvania, Romania. Valea Morii represents an isolated point on the distribution map of the species in Europe, and the single point where this species have been preserved until now in Romania. Tofieldia calyculata is integrated hare mainly in communities dominated by Schoenus nigricans.

  6. Geographic variation in the damselfish-red alga cultivation mutualism in the Indo-West Pacific

    Directory of Open Access Journals (Sweden)

    Watanabe Katsutoshi

    2010-06-01

    Full Text Available Abstract Background On coral reefs, damselfish defend their territories from invading herbivores and maintain algal turfs, from which they harvest filamentous algae. In southern Japan, intensive weeding of indigestible algae by Stegastes nigricans results in overgrowth by one filamentous alga, Polysiphonia sp. 1. Because this alga is highly susceptible to grazing and is competitively inferior to other algae, it survives only within the protective territories of this fish species, suggesting an obligate mutualism between damselfish and their cultivated alga. The wide distribution of damselfish species through the Indo-Central Pacific raises the question of whether this species-specific mutualism is maintained throughout the geographic range of the fish. To address this question, from all 18 damselfish species we conducted comprehensive surveys of algal flora within their territories throughout the Indo-West Pacific, and identified species of Polysiphonia using morphological examination and gene sequencing data. Results Several species of the genus Polysiphonia were observed as a major crop in territories throughout the geographic range of S. nigricans. Polysiphonia sp. 1 occurred only in territories of S. nigricans in central areas of the Indo-Pacific. However, its occurrence was low from the Great Barrier Reef and Mauritius. In contrast, other indigenous Polysiphonia species, which formed a clade with Polysiphonia sp. 1, occurred in the territories of fishes from Egypt, Kenya, and the Maldives. The other Polysiphonia species in the clade only inhabited damselfish territories and were never found elsewhere. Conclusions Cultivation mutualism between the damselfish S. nigricans and algae of Polysiphonia was maintained throughout the Indo-West Pacific, although algal crop species and the mode of cultivation (e.g., presence/absence of selective weeding, the species composition of algal turfs varied among localities. This finding implies that

  7. Novelties on taxonomy and nomenclature of Spanish vascular hybrids

    Directory of Open Access Journals (Sweden)

    Crespo, Manuel B.

    1990-05-01

    Full Text Available The nomenclature of some Iberian hybrids is revised and several new combinations are proposed: Centaurea X subdecurrens var. segobricensis, C. X subdecurrens notbosubsp. albuferae, Cistus X hybridus nothosubsp. grandiflorus var. secallianus. C. X ledon var. recognitus, C. X nigricans var. longifolius, C. X nigricans nothosubsp. grosii and Onopordum X humile var. turolensis. Besides, a new nothosubspecies: Sideritis x paui nothosubsp. enguerana (S. hirsuta X S. incana subsp. virgata, is described for the mountains of Valencia Province (E of Spain.

    Se revisa la nomenclatura de algunos híbridos vasculares ibéricos, proponiéndose algunas nuevas combinaciones: Centaurea X subdecurrens var. segobricensis, C. X subdecurrens notbosubsp. albuferae, Cistus X hybridus nothosubsp. grandiflorus var. secallianus. C. X ledon var. recognitus, C. X nigricans var. longifolius, C. X nigricans nothosubsp. grosii y Onopordum X humile var. turolensis. Además, se describe una nueva notosubespecie de las montañas de la provincia de Valencia: Sideritis X paui nothosubsp. enguerana (S. hirsuta X S. incana subsp. virgata.

  8. Examination of mycological samples by means of the scanning electron microscope

    Directory of Open Access Journals (Sweden)

    M. Thibaut

    1973-04-01

    Full Text Available Three species of Siphomycetes: Rhizopus arhizus, Rhizopus equinus and Rhizopus nigricans, as well as a Septomycete: Emericella nidulans, have been examined by means of a scanning electron microscope. Among the difjerent Rhizopus, this technique showed differences in the appearance of the sporangia. In Emericella nidulans, scanning microscopy enábled one to ascertain that the "Hull cells" were completely hollow and also demonstrated the ornemented aspect of the ascospores.

  9. Comparative ecology of widely distributed pelagic fish species in the North Atlantic: Implications for modelling climate and fisheries impacts

    DEFF Research Database (Denmark)

    Trenkel, V.M.; Huse, G.; MacKenzie, Brian

    2014-01-01

    scombrus), capelin (Mallotus villosus), blue whiting (Micromesistius poutassou), and horse mackerel (Trachurus trachurus), which have distributions extending beyond the continental shelf and predominantly occur on both sides of the North Atlantic. We also include albacore (Thunnus alalunga), bluefin tuna...... (Thunnus thynnus), swordfish (Xiphias gladius), and blue marlin (Makaira nigricans), which, by contrast, show large-scale migrations at the basin scale. We focus on the links between life history processes and the environment, horizontal and vertical distribution, spatial structure and trophic role. Many...

  10. Common descent of B chromosomes in two species of the fish genus Prochilodus (Characiformes, Prochilodontidae).

    Science.gov (United States)

    Voltolin, T A; Pansonato Alves, J C; Senhorini, J A; Foresti, F; Camacho, J P M; Porto-Foresti, F

    2013-01-01

    To ascertain the origin of B chromosomes in 2 fish species of the genus Prochilodus, i.e. P. lineatus and P. nigricans, we microdissected them and generated B-specific DNA probes. These probes were used to perform chromosome painting in both species and in 3 further ones belonging to the same genus (P. argenteus, P. brevis and P. costatus). Both probes hybridized with the B chromosomes in P. lineatus and P. nigricans, but with none of the chromosomes in the 5 species. This indicates that the B chromosomes have low similarity with DNAs located in the A chromosomes and suggests the possibility that the B chromosomes in the 2 species have a common origin. The most parsimonious explanation would imply intergeneric hybridization in an ancestor of P. lineatus and P. nigricans yielding the B chromosome as a byproduct, which remained in these 2 species after their phylogenetic origin, but was perhaps lost in other Prochilodus species. This hypothesis predicts that B chromosomes are old genomic elements in this genus, and this could be tested once a species from a relative genus would be found showing homology of its A chromosomes with the B-probes employed here, through a comparison of B chromosome DNA sequences with those in the A chromosomes of this other species.

  11. Focal epithelial hyperplasia: Case report.

    Science.gov (United States)

    Puriene, Alina; Rimkevicius, Arunas; Gaigalas, Mindaugas

    2011-01-01

    The purpose of the present article is to present a 15 year-old patient with focal epithelial hyperplasia and to review the references on the subject-related etiological, pathological, diagnostic and treatment aspects. Focal epithelial hyperplasia is a rare human papilloma virus (HPV) related to oral lesion with very low frequency within our population. Surgical treatment with a biopsy was performed, acanthosis and parakeratosis are consistent histopathological features, since the patient had no history of sexual contact and HIV infection, the virus was probably acquired from environmental sources.

  12. Eumycetoma Treated With Ketoconazole

    Directory of Open Access Journals (Sweden)

    Mittal Radha Rani

    1997-01-01

    Full Text Available A 43 year old female had progressive indolent, firm, tender, nodular swellings with multiple sinuses discharging pus containing black granules on left foot and ankle since 3 years. Smear of granule showed dark brown irregular mass with hyphae towards the periphery. Superimposed E. coli infection was treated with cefadroxyl 500 mg B.D Histopathologically marked acanthosis of epidermis , dense collection of mononuclear PMNL’s, plasma cells around granules of maduramycosis were seen. X-ray foor showed osteolytic lesions and culture was negative. Satisfactory and progressive improvement occurred after 7 weeks of therapy with ketoconozole 200 mg.

  13. Reticulated acanthoma with sebaceous differentiation. Lack of association with Muir-Torre syndrome.

    Science.gov (United States)

    Haake, Dana L; Minni, John P; Nowak, Michael; Abenoza, Pascual; Nousari, Carlos H

    2009-06-01

    We hereby report a case of a reticulated acanthoma with sebaceous differentiation (RASD), a rare and often mislabeled benign lesion that is characterized by epidermal acanthosis and clusters of sebocytes in a reticulated seborrheic keratosis-like pattern. The presence of multiple sebaceous tumors, most notably cystic sebaceous adenomas and keratoacanthomas, has been associated with Muir-Torre syndrome (MTS). Although very rare, cases of RASD have been reported with MTS, which potentially offers profound clinical significance to this neoplasm. This case further supports the lack of association of MTS with RASD.

  14. Comparative histopathology of scabies versus nodular scabies

    Directory of Open Access Journals (Sweden)

    Mittal R

    1997-01-01

    Full Text Available Comparative histopathology was studied in 25 cases of scabies versus 25 cases of nodular scabies which were selected from Dermato-Venereology out patients. Salient differences observed were that in scabies lifting of stratum corneum at places was seen in all 100% cases, spongiosis in 100%, spongiotic vesicles in 28%, burrows in 56%, mite in 40% and vasculitis in 28% whereas in nodular scabies acanthosis was seen in 100%, pseudo epitheliomatous hyperplasia in 8%, burrows in 48%, mite in 24% and vasculitis in 84%. In nodular scabies, dermal infiltrate in 32% cases was arranged as lymphoid follicles with admixture of plasma cells and eosinophils.

  15. A Case of Verruciform Periungual Fibroma%疣状甲周纤维瘤1例

    Institute of Scientific and Technical Information of China (English)

    高爱英; 金新东; 于小兵; 江瑛

    2013-01-01

    A 29-years-old female presented with periungual tumor on the left great toe for 7 years. The pathologic features consist of acanthosis, hyperkeratosis of epidermis, vascular proliferation and proliferation of dermal collagen fibers. Periungual fibroma was diagnosed.%患者女,29岁.左足拇趾的甲根部肿块7年.手术切除术物组织病理示:表皮角化过度,棘层肥厚,真皮胶原纤维和血管增生.诊断:甲周纤维瘤.

  16. Mange caused by Sarcoptes scabiei (Acari: Sarcoptidae) in wild raccoon dogs, Nyctereutes procyonoides, in Kanagawa Prefecture, Japan.

    Science.gov (United States)

    Takahashi, M; Nogami, S; Misumi, H; Maruyama, S; Shiibashi, T; Yamamoto, Y; Sakai, T

    2001-04-01

    Parasitological and histopathological examinations were performed in 25 raccoon dogs (Nyctereutes procyonoides) obtained in Kanagawa Prefecture, Japan, all of which were found to be heavily infected with Sarcoptes scabiei. The mites detected on these raccoon dogs were morphologically indistinguishable from the human species, and no Demodex mites were detected. Histopathological examinations showed prominent hyperkeratosis and acanthosis with eczema, and numerous burrows containing mites were observed in the epidermis. The enzootic dermatitis of wild raccoon dogs in recent years was clearly demonstrated to be caused by S. scabiei in the present study.

  17. Epidermization in the esophageal mucosa: unusual epithelial changes clearly detected by Lugol's staining.

    Science.gov (United States)

    Nakanishi, Y; Ochiai, A; Shimoda, T; Yamaguchi, H; Tachimori, Y; Kato, H; Watanabe, H; Hirohashi, S

    1997-05-01

    A 58-year-old Japanese man with superficial esophageal cancer accompanied by unusual epithelial changes, including esophageal mucosal epidermization, is reported. Staining with Lugol's iodine clearly showed irregular unstained lesions, which could not be seen clearly macroscopically, in the resected specimen. Histologic examination of the irregular unstained areas showed definite granular and horny layers regarded as epidermization, acanthosis with slight nuclear enlargement, and epithelial atrophy. The immunohistochemical staining patterns of keratins in the epidermized and atrophic lesions were similar to those in the epidermis, and the keratin staining patterns of the acanthotic lesion were similar to those of the oral epithelium.

  18. Cowden syndrome detected by FDG PET/CT in an endometrial cancer patient

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Yun Hee; Lee, Hye Kyung [Eulji University Hospital, Daejeon (Korea, Republic of); Park, Geon [Dept. of Radiology, The Catholic University of Korea, Daejeon Saint Mary' s Hospital, Daejeon (Korea, Republic of)

    2016-09-15

    Cowden syndrome (CS) is a rare autosomal dominant disorder characterized by multiple hamartomas in various tissues and cancers (breast, thyroid, and endometrium). We report CS of the esophagus and gastrointestinal tract that was incidentally detected by positron emission tomography/computed tomography (PET/CT) at postoperative surveillance in an endometrial cancer patient. PET/CT showed mildly increased FDG uptake along the entire esophagus and stomach. Upper GI endoscopy and histologic examination revealed glycogenic acanthosis of the esophagus and several hundred gastric polyps. In our case, increased FDG uptake of the esophageal wall contributed to the diagnosis of CS.

  19. Oral melanoacanthosis (melanoachantoma): report of a case and review of the literature.

    Science.gov (United States)

    Contreras, Elisa; Carlos, Román

    2005-01-01

    Oral melanoacanthosis (MA) is a rare pigmented mucosal lesion that is considered the counterpart of cutaneous melanoacanthoma. Microscopically the superficial epithelium shows mild to moderate acanthosis, spongiosis and prominent dendritic melanin producing melanocytes, which are present throughout the spinous keratinocytes. Reported cases show predilection for black females and the most common locations in decreasing frequency are buccal mucosa, lip, palate and gingiva. Although its pathogenesis remains uncertain, its clinical behavior is suggestive of a reactive origin. The clinical appearance of oral MA is non diagnostic and therefore biopsy is mandatory to differentiate from other melanocytic lesions, including melanoma.

  20. Identification of fungal diseases associated with imported wheat in Iranian silos.

    Science.gov (United States)

    Okhovvat, S M; Zakeri, Z

    2003-01-01

    In this study, imported wheat varieties used for cookies and bread making were evaluated for the presence of fungal diseases in the silos. Grain samples were taken and cultured on nutrient agar medium and sterile papers impregnated with nutrient. The results showed the presence of pathogenic fungi such as Ulocladium sp., Cladosporium sp., Alternaria sp., Rhizopus nigricans, Penicillium sp. and Trichothecium sp. in varieties from Australian, Mucor sp., R. nigricans, Fusarium sp., A. triticum, Helminthosporium sp. and Penicillium sp. from Argentina, Alternaria sp., Ulocladium sp., Penicillium sp., Aspergillus sp., Mucor mucedo, R. nigricans, Fusorium sp., Curvularia triticola, U. clamydosporium and C. tritici from Kazakistan varieties stored in Karaj silos or unloading trains. It is noteworthy to mention that Fusarium sp., Helminthosporium sp., Alternaria sp., A. tritici, A. triticola and U. clamydosporium are phytopathogenic fungi that often cause serious diseases on crops, produce lots of spores that are widely disseminated across the field and grow and reproduce in plant residues and diseased or wounded plant tissues and mature grains particularly under moist conditions. If in case, farmers try to use contaminated wheat grains that are distributed among them for flour, for cultivation purposes, it is highly probable that new fungal strains and species will be introduced in the areas where wheat production has never been threatened before. Fungal disease such as Indian smut or rusts is not native to Iran but are considered quarantine diseases. In addition, high incidence of contamination due to the presence of mycotoxins produced by Penicillium sp. and Aspergillus sp. in foreign wheat cultivars, could result in serious toxicity and illness in humans and birds.

  1. Do biopesticides affect the demographic traits of a parasitoid wasp and its biocontrol services through sublethal effects?

    Directory of Open Access Journals (Sweden)

    Antonio Biondi

    Full Text Available Pesticide risk assessments are usually based on short-term acute toxicity tests, while longer-term population dynamic related traits, critical to the success of biological control and Integrated Pest Management (IPM programs, are often overlooked. This is increasingly important with respect to new biopesticides that frequently cause no short-term acute effects, but that can induce multiple physiological and behavioral sublethal effects, leading to a decrease in population growth and ecosystem services. In this study we assessed the lethal and sublethal effects of six biopesticides [abamectin, azadirachtin, Bacillus thuringiensis, borax plus citrus oil (Prev-Am®, emamectin benzoate, and spinosad], used in tomato crops to control the invasive pest Tuta absoluta (Lepidoptera: Gelechiidae, on adults and pupae of the parasitoid Bracon nigricans (Hymenoptera: Braconidae. Data on female survival and production of female offspring were used to calculate population growth indexes as a measure of population recovery after pesticide exposure. Spinosad caused 100% and 80% mortality in exposed adults (even 10 d after the treatment and pupae, respectively. Although most of the biopesticides had low levels of acute toxicity, multiple sublethal effects were observed. The biocontrol activity of both females that survived 1-h and 10-d old residues, and females that emerged from topically treated pupae was significantly affected by the application of the neurotoxic insecticides emamectin benzoate and abamectin. Furthermore, very low B. nigricans demographic growth indices were estimated for these two insecticides, indicating potential local extinction of the wasp populations. Among the tested products, Bt proved to be the safest for B. nigricans adults and pupae. Our findings emphasize that acute toxicity assessment alone cannot fully predict the actual impact of pesticides on non-target parasitoids. Thus, sublethal effects related to the species specific life

  2. New geographic records of Hamlets, Hypoplectrus spp. (Serranidae), in the Caribbean Sea

    Science.gov (United States)

    Williams, E.H.; Bunkley-Williams, L.; Rogers, C.S.; Fenner, R.

    2006-01-01

    The exact number of species of hamlets, Hypoplectrus spp., in the Caribbean is controversial and the geographic distributions of these species/forms are poorly documented. We report Curac??ao, Netherlands Antilles, as a new locality for the Barred Hamlet, H. puella (Cuvier), and Shy Hamlet, H. guttavarius (Poey); and St. John and St. Thomas, U.S. Virgin Islands, for the Tan Hamlet, Hypoplectrus sp. The Black Hamlet, H. nigricans (Poey), has previously been reported from Curac??ao, but we did not see it there.

  3. Diabetes tipo 2 en niños. Reporte de caso

    OpenAIRE

    Pinto, Miguel; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia Lima.; Seclén, Segundo; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Lima.; Cabello, Emilio; Unidad de Endocrinología Pediátrica, Hospital Nacional Cayetano Heredia. Lima.

    2011-01-01

    Se reporta el caso de una niña de 14 años con antecedentes de obesidad, dislipidemia y diabetes que es referida a nuestro servicio por polidipsia, poliuria y sobrepeso. La paciente tenía el antecedente familiar de ambos padres con diagnóstico de diabetes tipo 2. El examen físico mostró obesidad central y acantosis nigricans severa en los pliegues cutáneos. Los análisis de laboratorio mostraron hiperglicemia, hiperinsulinemia y pép...

  4. Biogenic amine content, histamine-forming bacteria, and adulteration of pork in tuna sausage products.

    Science.gov (United States)

    Kung, Hsien-Feng; Tsai, Yung-Hsiang; Chang, Shih-Chih; Hong, Tang-Yao

    2012-10-01

    Twenty-five tuna sausage products were purchased from retail markets in Taiwan. The rates of occurrence of biogenic amines, histamine-forming bacteria, and adulteration by pork and poultry were determined. The average content of various biogenic amines in all tested samples was less than 2.0 mg/100 g (sausage. The fish species in the tuna sausage samples were identified as Thunnus albacares for 22 samples (88%), Thunnus alalunga for 1 sample (4%), and Thunnus thynnus for 1 sample (4%), whereas the remaining sample was identified as Makaira nigricans (blue marlin).

  5. The identity of Myotis punensis (Chiroptera: Vespertilionidae

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    Ricardo Moratelli

    2011-02-01

    Full Text Available Currently twelve species of Myotis Kaup, 1829 (Vespertilionidae: Myotinae are recognized from South America, with several other named taxa regarded as synonyms, among them Myotis punensis J.A. Allen, 1914. This name was first regarded as a junior synonym of Myotis albescens (É. Geoffroy, 1806 and subsequently of Myotis nigricans (Schinz, 1821. To address the taxonomic status of the holotype of M. punensis, we compared it to all South American species in the genus. The fringe of hairs on the trailing edge of the uropatagium, the fur color, and external and skull dimensions all suggest M. punensis should be treated as a junior synonym of M. albescens

  6. Surgeons and suture zones: Hybridization among four surgeonfish species in the Indo-Pacific with variable evolutionary outcomes

    KAUST Repository

    DiBattista, Joseph

    2016-04-30

    Closely related species can provide valuable insights into evolutionary processes through comparison of their ecology, geographic distribution and the history recorded in their genomes. In the Indo-Pacific, many reef fishes are divided into sister species that come into secondary contact at biogeographic borders, most prominently where Indian Ocean and Pacific Ocean faunas meet. It is unclear whether hybridization in this contact zone represents incomplete speciation, secondary contact, an evolutionary dead-end (for hybrids) or some combination of the above. To address these issues, we conducted comprehensive surveys of two widely-distributed surgeonfish species, Acanthurus leucosternon (N = 141) and A. nigricans (N = 412), with mtDNA cytochrome b sequences and ten microsatellite loci. These surgeonfishes are found primarily in the Indian and Pacific Oceans, respectively, but overlap at the Christmas and Cocos-Keeling Islands hybrid zone in the eastern Indian Ocean. We also sampled the two other Pacific members of this species complex, A. achilles (N = 54) and A. japonicus (N = 49), which are known to hybridize with A. nigricans where their ranges overlap. Our results indicate separation between the four species that range from the recent Pleistocene to late Pliocene (235,000 to 2.25 million years ago). The Pacific A. achilles is the most divergent (and possibly ancestral) species with mtDNA dcorr ≈ 0.04, whereas the other two Pacific species (A. japonicus and A. nigricans) are distinguishable only at a population or subspecies level (ΦST = 0.6533, P < 0.001). Little population structure was observed within species, with evidence of recent population expansion across all four geographic ranges. We detected sharing of mtDNA haplotypes between species and extensive hybridization based on microsatellites, consistent with later generation hybrids but also the effects of allele homoplasy. Despite extensive introgression, 98% of specimens had concordance between mt

  7. Surgeons and suture zones: Hybridization among four surgeonfish species in the Indo-Pacific with variable evolutionary outcomes.

    Science.gov (United States)

    DiBattista, Joseph D; Whitney, Jonathan; Craig, Matthew T; Hobbs, Jean-Paul A; Rocha, Luiz A; Feldheim, Kevin A; Berumen, Michael L; Bowen, Brian W

    2016-08-01

    Closely related species can provide valuable insights into evolutionary processes through comparison of their ecology, geographic distribution and the history recorded in their genomes. In the Indo-Pacific, many reef fishes are divided into sister species that come into secondary contact at biogeographic borders, most prominently where Indian Ocean and Pacific Ocean faunas meet. It is unclear whether hybridization in this contact zone represents incomplete speciation, secondary contact, an evolutionary dead-end (for hybrids) or some combination of the above. To address these issues, we conducted comprehensive surveys of two widely-distributed surgeonfish species, Acanthurus leucosternon (N=141) and A. nigricans (N=412), with mtDNA cytochrome b sequences and ten microsatellite loci. These surgeonfishes are found primarily in the Indian and Pacific Oceans, respectively, but overlap at the Christmas and Cocos-Keeling Islands hybrid zone in the eastern Indian Ocean. We also sampled the two other Pacific members of this species complex, A. achilles (N=54) and A. japonicus (N=49), which are known to hybridize with A. nigricans where their ranges overlap. Our results indicate separation between the four species that range from the recent Pleistocene to late Pliocene (235,000-2.25million years ago). The Pacific A. achilles is the most divergent (and possibly ancestral) species with mtDNA dcorr≈0.04, whereas the other two Pacific species (A. japonicus and A. nigricans) are distinguishable only at a population or subspecies level (ΦST=0.6533, P<0.001). Little population structure was observed within species, with evidence of recent population expansion across all four geographic ranges. We detected sharing of mtDNA haplotypes between species and extensive hybridization based on microsatellites, consistent with later generation hybrids but also the effects of allele homoplasy. Despite extensive introgression, 98% of specimens had concordance between mtDNA lineage and

  8. Mildew fungi found in termites (Reticulitermes lucifugus and their nests

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    A. Wójcik

    2015-04-01

    Full Text Available This paper presents the results of observation of mould growth in laboratory colonies of termites. It also attempts to determine the species of mould fungi present in the research laboratory and the main colonies and their entomopathogenic for the termites. The following four species were found in test termite colonies: Trichoderme viride, Mucor himeralis, Rhizopus nigricans, Aspergillus sp., Aspergillus flavus, Alternaria sp., Penicylium verucosum and Fusarium sp. were recognisable in test colonies with domestic and exotic wood. Morphological observations of the fungi were carried out using a microscope with a 40x magnification. The growth of mould fungi in test containers caused death of whole termite colonies.

  9. Sludy of the Dermatophytes in the Students Houses of Minia University, Egypt

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    S. N. Maghazy

    2014-08-01

    Full Text Available A survey of dermatophytes and other fungi was carried out in 100 air - dust samples from bedrooms and dinning halls of male and female student resident houses. By hair baiting technique the common dermatophytes were obtained namely Microsporum canis, M. gypseum and Trichophyton mtntagrophytes. Also five species of Chrysasporium were isolated in the following order of dominance C. tropicum, C. keratinophilum, C. indicum, C. pannicola and C. quecnslandicum. By dilution plate method, 37 species representing 20 genera of which Aspergilus niger, A. flavus, Rhizopus nigricans, Penicillium chrysogenum and Cladosporium cladosporioides were most frequently isolated.

  10. Molecular phylogenetics of the Neotropical fish family Prochilodontidae (Teleostei: Characiformes).

    Science.gov (United States)

    Melo, Bruno F; Sidlauskas, Brian L; Hoekzema, Kendra; Frable, Benjamin W; Vari, Richard P; Oliveira, Claudio

    2016-09-01

    Migratory detritivores of the characiform family Prochilodontidae occur throughout the freshwaters of much of South America. Prochilodontids often form massive populations and many species achieve substantial body sizes; a combination that makes them one of the most commercially important fish groups on the continent. Their economic significance notwithstanding, prochilodontids have never been the subject of a comprehensive molecular phylogenetic analysis. Using three mitochondrial and three nuclear loci spanning all prochilodontid species, we generated a novel phylogenetic hypothesis for the family. Our results strongly support monophyly of the family and the three included genera. A novel, highly supported placement of Ichthyoelephas sister to the clade containing Prochilodus and Semaprochilodus diverges from a previous morphological hypothesis. Most previously hypothesized interspecific relationships are corroborated and some longstanding polytomies within Prochilodus and Semaprochilodus are resolved. The morphologically similar P. brevis, P. lacustris, P. nigricans and P. rubrotaeniatus are embedded within what is herein designated as the P. nigricans group. Species limits and distributions of these species are problematic and the group clearly merits taxonomic revision.

  11. Intra- and interspecific aggression do not modulate androgen levels in dusky gregories, yet male aggression is reduced by an androgen blocker.

    Science.gov (United States)

    Vullioud, Philippe; Bshary, Redouan; Ros, Albert Frank Huascar

    2013-08-01

    Discussions about social behavior are generally limited to fitness effects of interactions occurring between conspecifics. However, many fitness relevant interactions take place between individuals belonging to different species. Our detailed knowledge about the role of hormones in intraspecific interactions provides a starting point to investigate how far interspecific interactions are governed by the same physiological mechanisms. Here, we carried out standardized resident-intruder (sRI) tests in the laboratory to investigate the relationship between androgens and both intra- and interspecific aggression in a year-round territorial coral reef fish, the dusky gregory, Stegastes nigricans. This damselfish species fiercely defend cultivated algal crops, used as a food source, against a broad array of species, mainly food competitors, and thus represent an ideal model system for comparisons of intra-and interspecific territorial aggression. In a first experiment, resident S. nigricans showed elevated territorial aggression against intra- and interspecific intruders, yet neither elicited a significant increase in androgen levels. However, in a second experiment where we treated residents with flutamide, an androgen receptor blocker, males but not females showed decreased aggression, both towards intra- and interspecific intruders. Thus androgens appear to affect aggression in a broader territorial context where species identity of the intruder appears to play no role. This supports the idea that the same hormonal mechanism may be relevant in intra- and interspecific interactions. We further propose that in such a case, where physiological mechanisms of behavioral responses are found to be context dependent, interspecific territorial aggression should be considered a social behavior.

  12. C11 α-hydroxylation of C21 Steroidal Aglycones in Cynanchum Aurieulatum by Microbial Simultaneously Transformation%白首乌C21甾苷元C11α-羟化的两种微生物同步转化

    Institute of Scientific and Technical Information of China (English)

    李于善; 贺艳

    2009-01-01

    目的 对白首乌C21甾苷元告达庭甾苷元和开德甾苷元(Ⅱ)实现C11 α-羟基化,要求副产物少,收率高.方法 采用黑根霉(Rhizopus nigricans)和犁头霉(Absidia sp.)两种微生物在水-正丁醇双相体系中同步转化的方法,羟化反应中控制值pH在5.0~6.0,于30℃温度下振荡培养70 h.结果 产物经IR、MS、NMR分析确认是C11 α-羟基化了的告达庭甾苷元(Ⅲ)和开德甾苷元(Ⅲ),副产物少,收率达70.1%.结论 用黑根霉(Rhizopus nigricans)和犁头霉(Absidia sp.)两种微生物在水-正丁醇双相体系中同步转化的方法是实现C11 α-羟基化的有效方法.

  13. Oral focal epithelial hyperplasia: report of 3 cases with human papillomavirus DNA sequencing analysis.

    Science.gov (United States)

    Gültekin, S E; Tokman Yildirim, Benay; Sarisoy, S

    2011-01-01

    Focal epithelial hyperplasia (FEH), or Heck's disease, is a benign proliferative viral infection of the oral mucosa that is related to Human Papil-lomavirus (HPV), mainly subtypes 13 and 32. Although this condition is known to exist in numerous populations and ethnic groups, the reported cases among Caucasians are relatively rare. It presents as asymptomatic papules or nodules on the oral mucosa, gingiva, tongue, and lips. Histopathologically, it is characterized by parakeratosis, epithelial hyperplasia, focal acanthosis, fusion, and horizontal outgrowth of epithelial ridges and the cells named mitozoids. The purpose of this case report was to present 3 cases of focal epithelial hyperplasia in a pediatric age group. Histopathological and clinical features of cases are discussed and DNA sequencing analysis is reported in which HPV 13, HPV 32, and HPV 11 genomes are detected.

  14. Atopic dermatitis. Findings of skin biopsies.

    Science.gov (United States)

    Piloto Valdés, L; Gómez Echevarría, A H; Valdés Sánchez, A F; Ochoa Ochoa, C; Chong López, A; Mier Naranjo, G

    1990-01-01

    Twenty-eight adult patients with a clinical diagnosis of atopic dermatitis (according to the criteria of Hanifin and Lobitz) were studied at the Allergy Outpatient Service, the Dermatology Service and the Pathological Anatomy Service of the Hermanos Ameijeiras Clinical Surgical Hospital, from January to September 1986. The patients were submitted to a quantification of total serum IgE by means of the ELISA enzymatic ultramicromethod, developed at the Radioimmunoassay National Center, and skin biopsies were carried out by means of the paraffin and direct immunofluorescence methods. The most frequent histopathological findings were acanthosis, espongiosis, parakeratosis and exocitosis, as a chronic inflammatory infiltrate, mainly composed of lymphocytes, mast cells and eosinophils. In the skin direct immunofluorescence method we found depots of IgE in all the patients, having no relation in intensity to total serum IgE values.

  15. Diesel oil-induced alopecia in two cats.

    Science.gov (United States)

    Declercq, Jan; De Bosschere, Hendrik

    2009-04-01

    Two cats were presented for acute onset of rapidly progressive, bilaterally symmetrical hair loss of the ventrum and limbs. Alopecia occurred within 2 weeks after accidental skin exposure to diesel oil. The remaining hair epilated easily in affected areas. Denuded skin was strikingly dry and had adherent scale. Erythema and demarcation between affected and normal skin by a liquid-line were present in one case. Above this line, the hair could not be removed. The head and the footpads were not involved. Systemic signs were not observed. Both cats made a complete recovery without treatment. Histological examination in one cat revealed severe orthokeratotic hyperkeratosis, mild to moderate acanthosis, follicular keratosis, a moderate dermal infiltrate of mast cells and an almost complete absence of sebaceous glands. If skin contact with diesel oil occurs in a cat, thorough washing of skin and haircoat after soaking in prue vegetable oil is recommended.

  16. Diffuse intestinal ganglioneuromatosis an uncommon manifestation of Cowden syndrome.

    Science.gov (United States)

    Herranz Bachiller, Maria Teresa; Barrio Andrés, Jesus; Pons, Fernando; Alcaide Suárez, Noelia; Ruiz-Zorrilla, Rafael; Sancho Del Val, Lorena; Lorenzo Pelayo, Sara; De La Serna Higuera, Carlos; Atienza Sánchez, Ramon; Perez Miranda, Manuel

    2013-02-15

    Diffuse intestinal ganglioneuromatosis is a hamartomatous polyposis characterized by a disseminated, intramural or transmural proliferation of neural elements involving the enteric plexuses. It has been associated with MEN II, neurofibromatosis type 1 and hamartomatous polyposis associated with phosphatase and tensin homolog mutation. We report the case of a female patient with a history of a breast and endometrial tumor who presented in a colonoscopy performed for rectal bleeding diffuse ganglioneuromatosis, which oriented the search for other characteristic findings of Cowden syndrome given the personal history of the patient. The presence of an esophagogastric polyposis was also noted. Cowden syndrome is characterized by skin lesions, but it is rarely diagnosed by these lesions, because they are usually overlooked. Intestinal polyposis is not a major diagnostic criterion but it is very useful for early diagnosis. The combination of colonic polyposis and glucogenic acanthosis should orient the diagnosis to Cowden syndrome.

  17. Melanoacanthoma: Uncommon presentation of an uncommon condition

    Directory of Open Access Journals (Sweden)

    Resham J Vasani

    2013-01-01

    Full Text Available Melanoacanthoma is very rare variant of seborrheic keratosis presenting as a deeply pigmented benign proliferation of melanocytes and keratinocytes usually presenting over the head, neck and trunk of elderly people. A sixty-two-years-old male was presented with a solitary slow growing asymptomatic hyperpigmented verrucous outgrowth with cerebriform surface measuring 15 cm by 8 cm present over the left inguinal region extending on to the scrotum since past 8 years. There was no associated lymphadenopathy. The histopathology revealed hyperkeratosis, papillomatosis, acanthosis with presence of melanocytes at all levels of epidermis with abundant melanin giving the diagnosis of melanoacanthoma. The patient further underwent surgical excision of the lesion. The case is being reported for its rarity, unusual location, massive size and clinical resemblance to a verrucous carcinoma.

  18. Leukokeratosis nicotina glossi-smokers' tongue.

    Science.gov (United States)

    Farman, A G; Van Wyk, C W

    1977-12-01

    "Leukokeratosis nicotina glossi" or "smokers' tongue" is a homogeneous leukoplakia with evenly distributed pin-point hemispherical depressions. Histologically, there is a loss of glossal papillae, hyperkeratosis, acanthosis and the formation of large drop-shaped rete pegs with central clefting and occasional parakeratotic plugging. Mitotic activity and atypia are not marked and there is no evidence of Candida species infection. In some respects the lesion histologically resembles verrucous carcinoma but, unlike that condition, papillomatosis is not clinically noticeable and an invasive "leading edge" is not apparent. All but one of the subjects in which the lesion was seen were men, all had concurrent leukokeratosis nicotina palati and two gave histories of laryngeal carcinoma.

  19. Histopathological study of the mite biting (Dermanyssus gallinae in poultry skin

    Directory of Open Access Journals (Sweden)

    Rahim Hobbenaghi

    2012-09-01

    Full Text Available The red mite of poultry, Dremanyssus gallinae, is the most important hematophagous ectoparasite of poultry. In this study, pathologic changes of its biting on the poultry skin have been investigated. Thirty-two (Control = 16 and Treatment = 16 four weeks old Ross broilers (308 were infested with the mite on skin of hock joins. Samples were collected after 1, 24, 72 hours and 10 days. The skin samples were fixed in 10% buffered formalin and histological sections were prepared using routine Hematoxylin & Eosin staining method. Results showed that in all cases, except within first hour of infestation, lymphocytic infiltration was always a constant pathologic feature. Necrosis of feather's follicles was a prominent pathologic feature ensued due to vascular disturbances and resulted in loss of feather. Hyperkeratosis, parakeratosis and acanthosis were observed after 72 hours. These findings reveal that mite biting induces local epidermal hyperplasia.

  20. Histopathological study of the mite biting (Dermanyssus gallinae) in poultry skin.

    Science.gov (United States)

    Hobbenaghi, Rahim; Tavassoli, Mousa; Alimehr, Manochehr; Shokrpoor, Sara; Ghorbanzadeghan, Mohammad

    2012-01-01

    The red mite of poultry, Dremanyssus gallinae, is the most important hematophagous ectoparasite of poultry. In this study, pathologic changes of its biting on the poultry skin have been investigated. Thirty-two (Control = 16 and Treatment = 16) four weeks old Ross broilers (308) were infested with the mite on skin of hock joins. Samples were collected after 1, 24, 72 hours and 10 days. The skin samples were fixed in 10% buffered formalin and histological sections were prepared using routine Hematoxylin & Eosin staining method. Results showed that in all cases, except within first hour of infestation, lymphocytic infiltration was always a constant pathologic feature. Necrosis of feather's follicles was a prominent pathologic feature ensued due to vascular disturbances and resulted in loss of feather. Hyperkeratosis, parakeratosis and acanthosis were observed after 72 hours. These findings reveal that mite biting induces local epidermal hyperplasia.

  1. Keratinization Disorders and Genetic Aspects in Palmar and Plantar Keratodermas.

    Science.gov (United States)

    Stypczyńska, Ewa; Placek, Waldemar; Zegarska, Barbara; Czajkowski, Rafał

    2016-06-01

    Palmoplantar keratoderma (PPK) is a heterogeneous group of hereditary and acquired disorders characterized by abnormal thickening of the palms and soles. There are three clinical patterns: diffuse, focal, and punctuate. Palmoplantar keratodermas can be divided into the following functional subgroups: disturbed gene functions in structural proteins (keratins), cornified envelope (loricrin, transglutaminase), cohesion (plakophilin, desmoplakin, desmoglein 1), cell-to-cell communication (connexins) and transmembrane signal transduction (cathepsin C). Unna-Thost disease is the most common variety of hereditary PPK. Mutations in keratin 1 have been reported in Unna-Thost disease. We report 12 cases in which Unna-Thost disease was diagnosed. Genealogical study demonstrated that the genodermatosis was a familial disease inherited as an autosomal dominant disorder. Dermatological examination revealed yellowish hyperkeratosis on the palms and soles. Oral mucosa, teeth, and nails remained unchanged. Histopathological examination of the biopsy sample taken from the soles of the patients showed orthokeratotic keratosis, hypergranulosis, and acanthosis without epidermolysis.

  2. Seronegative necrolytic acral erythema: A distinct clinical subset?

    Directory of Open Access Journals (Sweden)

    Panda S

    2010-01-01

    Full Text Available A patient was referred to us with asymptomatic, erythematous, nonitchy, scaly lesions present bilaterally on the dorsa of his feet and toes since the last 2 months. Both the legs had pitting edema as well. There were hyperkeratosis, focal parakeratosis, acanthosis and scattered spongiosis in the epidermis, and proliferation of capillaries with perivascular infiltration of lymphomononuclear cells in the dermis. There was no serological evidence of hepatitis C virus. Laboratory investigations revealed hypoalbuminemia and low-normal serum zinc. On clinicopathological correlation, we made a diagnosis of necrolytic acral erythema (NAE. The lesions responded dramatically to oral zinc sulfate and topical clobetasol propionate within 3 weeks with disappearance of edema and scaling and only a minimal residual erythema. This is the first reported case of NAE from Eastern India. NAE with negative serology for hepatitis C may be viewed as a distinct subset of the condition that had been originally described.

  3. Linear and whorled nevoid hypermelanosis complicated with inflammatory linear verrucous epidermal nevus and ichthyosis vulgaris.

    Science.gov (United States)

    Lu, Yan; Zhu, Wen-Yuan

    2007-11-01

    We report a 17-year-old girl who presented with linear and whorled melanosis following Blaschko lines mainly on her trunk. Ichthyosiform lesions and linear scaling erythemas were observed respectively on her lower limbs and the dorsa of her hands, left knee, ankle and foot. No abnormality was found in systemic examination and blood tests. A biopsy specimen of pigmentation of her back showed there was increased pigmentation within the basal keratinocytes, with focal incontinentia pigmenti. Notable lymphangiectasis could also be seen in the mid part of dermis, which had never previously been described. The biopsy of scaling erythemas of her hand showed dominant hyperkeratosis with focal parakeratosis, acanthosis and papillomatous hyperplasia in the epidermis. The diagnosis of linear and whorled nevoid hypermelanosis (LWNH) complicated with inflammatory linear verrucous epidermal nevus and ichthyosis vulgaris was made in accordance with clinical and pathological manifestations. LWNH ought to be differentiated from incontinentia pigmenti and hypomelanosis of Ito.

  4. Confluent And Reticulate Papillomatosis

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    Thappa Devinder Mohan

    2002-01-01

    Full Text Available A 17 year old South Indian female born of consanguineous marriage presented with a widespread non-itchy eruption over scalp, face, below breasts and flexural aspects of limbs including axillae, inguinal regions and popliteal fossae since 3 months of age. The eruption consisted of individual small, flat, brownish black hyperkeratotic papules, which at some places, coalesced to from a reticulate pattern. Histopathological examination of biopsies from the axilla and forearm showed typical hyperkeratosis and papillomatosis without acanthosis. The dermis demonstrated a mild perivascular infiltrate. On the basis of the clinical and histopathological findings, a diagnosis of confluent and reticulate papillomatosis of Gourgerot and Carteaud was made. Partial response to doxycline in our case point towards this being a keratinization disorder where microflora may be playing an exacerbating rather than a primary role.

  5. Hereditary epidermolytic palmoplantar keratoderma (Vörner type) in a family with Ehlers-Danlos syndrome.

    Science.gov (United States)

    Mofid, M Z; Costarangos, C; Gruber, S B; Koch, S E

    1998-05-01

    We describe a kindred in whom epidermolytic palmoplantar keratoderma occurred in association with Ehlers-Danlos syndrome type III (benign hypermobility syndrome). This kindred consisted of 27 members of four generations, 14 of whom had palmoplantar keratoderma (PPK). Of those who had palmoplantar keratoderma, 6 had Ehlers-Danlos type III (EDS II). The proband presented with diffuse, symmetrical hyperkeratotic plaques that were yellow and sharply demarcated, covering the entire palms and soles, in addition to marked large and small joint flexibility and skin hyperextensibility. A biopsy specimen from the palm revealed features of epidermolytic hyperkeratosis with acanthosis. To our knowledge, this is the first report of PPK in a family with Ehlers-Danlos syndrome. Linkage analysis of these two clinical traits showed that the genes responsible for PPK and EDS III are not closely linked, and therefore are not immediately adjacent. However, linkage at greater genetic distances could not be excluded.

  6. Granuloma inguinale mimicking as squamous cell carcinoma of penis

    Directory of Open Access Journals (Sweden)

    Abhishek Pilani

    2014-01-01

    Full Text Available Granuloma inguinale (GI is an acquired chronic, slowly progressive, mildly contagious disease of venereal origin, characterized by granulomatous ulceration of the genitalia and neighboring sites, with little or no tendency to spontaneous healing caused by Klebsiella (Calymmatobacterium granulomatis. A 55-year-old male presented with fissured, foul smelling, fungating growth over prepuce with phimosis mimicking squamous cell carcinoma (SCC without lymphadenopathy. It started with painless papulonodular showed pseudoepitheliomatous hyperplasia, infiltration in dermis, acanthosis and vacuolated macrophages suggestive of GI and not showing any histopathological features of SCC. Patient was successfully treated by giving cotrimoxazole twice a day for 21 days. Here, we presented a case of GI mimicking SCC of penis, which was diagnosed on basis of histopathology and treated with excision followed by medical therapy with cotrimoxazole.

  7. Nevo epidérmico manifestando-se como hiperceratose do mamilo e aréola Verrucous epidermal nevus manifesting as nipple and areola hyperkeratosis

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    Roberto Rheingantz da Cunha Filho

    2006-08-01

    Full Text Available Relata-se caso raro de nevo epidérmico na aréola mamária direita de menina de 13 anos. Segundo a classificação proposta por Levy-Franckel, essa variante é a hiperceratose do mamilo e aréola do tipo I, quando associada a nevo verrucoso. A histologia mostrou papilomatose, acantose e hiperceratose. Foram realizadas duas sessões de crioterapia sem melhora. Foi obtido bom resultado por meio de shaving e eletrocauterização.A rare case of a 13-year-old female patient with epidermal verrucous nevus on the right areola is reported. According to the Levy-Franckel classification, this variant is a type I nipple and areola hyperkeratosis, when associated to verrucous nevus. Histopathological examination showed papillomatosis, acanthosis and hyperkeratosis. Cryotherapy yielded unsatisfactory results after two sessions. A good result was obtained with shaving and electrocauterization.

  8. Condylomatous genital lesions in cynomolgus macaques from Mauritius.

    Science.gov (United States)

    Harari, Ariana; Wood, Charles E; Van Doorslaer, Koenraad; Chen, Zigui; Domaingue, Marie Claire; Elmore, David; Koenig, Patricia; Wagner, Janice D; Jennings, Ryan N; Burk, Robert D

    2013-08-01

    Genital condyloma-like lesions were observed on male and female cynomolgus macaque monkeys (Macaca fascicularis) originating from the island of Mauritius. Cytobrush and/or biopsy samples were obtained from lesions of 57 affected macaques. Primary histologic features included eosinophilic, neutrophilic, and lymphoplasmacytic penile and vulvar inflammation, epidermal hyperplasia with acanthosis, and increased collagenous stroma. Polymerase chain reaction-based assays to amplify viral DNA revealed the presence of macaque lymphocryptovirus (LCV) DNA but not papillomavirus or poxvirus DNA. Subsequent DNA analyses of 3 genomic regions of LCV identified isolates associated with lesions in 19/25 (76%) biopsies and 19/57 (33%) cytology samples. Variable immunolabeling for proteins related to the human LCV Epstein Barr Virus was observed within intralesional plasma cells, stromal cells, and epithelial cells. Further work is needed to characterize the epidemiologic features of these lesions and their association with LCV infection in Mauritian-origin macaques.

  9. Establishment of Mus Skin Photo-damage Model by 8-MOP plus UVA Irradiation

    Institute of Scientific and Technical Information of China (English)

    LIANG Hong; LI Jiawen; ZHANG Li

    2007-01-01

    To establish a simple and reliable animal model of skin photo-damage, 20 mice were treated with 8-MOP and exposed to UVA (UVA 320-400 nm) for 24 h. After irradiation, the structure of the epidermis and dermis, collagen fibers, elastic fibers were observed by using HE staining and Weigert technique and compared with the normal controls. The acanthosis and epidemis proliferation with accompanying hyperkeratosis and parakeratosis were observed. Inflammatory infiltration was noted in the dermis. The elastic fibers became coarse, irregularly arranged and clustered, with their number increased. The collagen fibers showed obvious degeneration and some amorphous materials could also be observed. The blood vessels were irregularly dilated and vascular walls were thickened, with infiltration of inflammatory cells. It is concluded that murine photodamage model can be quickly, conveniently and reliably established by means of 8-MOP/UVA.

  10. An unusual clinical presentation of gingival melanoacanthoma

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    S. P. K. Kennedy Babu

    2013-01-01

    Full Text Available Gingival melanoacanthoma is a rare, benign pigmented lesion characterized clinically by sudden onset and rapid growth of a macular brown black lesion and histologically by acanthosis of superficial epithelium and proliferation of dendritic melanocytes. This article reports a previously undescribed case of pigmented unilateral diffuse gingival enlargement, which on histopathological examination proved to be melanoacanthoma. Intraoral examination revealed pigmented unilateral diffuse gingival enlargement in relation to second and third quadrants buccally, palatally/lingually. Based on these clinical findings, gingivectomy was performed and the excised tissue was sent for biopsy. Microscopic examination revealed acanthotic and parakeratotic surface epithelium with dendritic melanocytes distributed in basal and suprabasal layers of the epithelium. 1 year follow-up recall revealed no recurrence of lesion at the surgical sites. Our patient exhibits an unusual clinical presentation of melanoacanthoma of gingiva. Pigmented gingival overgrowth of recent origin and without any etiologic factors warrants histopathologic examination.

  11. Evaluation of clinical and laboratory markers of cardiometabolic risk in overweight and obese children and adolescents

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    Heloísa Marcelina da Cunha Palhares

    Full Text Available OBJECTIVE: This study analyzed the frequency of cardiometabolic risk markers and metabolic syndrome occurrence in overweight and obese children and adolescents. METHODS: The participants included 161 overweight (n=65 and obese (n=96 individuals aged between 5 and 19 years. Clinical markers were assessed (body mass index, body fat percentage, waist circumference, acanthosis, systolic and diastolic blood pressures, laboratory parameters [glucose, insulin, cholesterol (total and fractions and triglyceride levels and homeostasis model assessment of insulin resistance (HOMA-IR index] and leptin and adiponectin levels. The frequency of changes, odds ratios and correlations among markers were determined. Metabolic syndrome was assessed according to International Diabetes Federation criteria. RESULTS: A high frequency of acanthosis (51.6%; increased waist circumference (45.4%, systolic blood pressure / diastolic blood pressure (8.1% / 9.3%, glucose (10%, insulin (36.9% and HOMA-IR (44.3% values; and reduced high-density lipoprotein levels (47.2% were observed. Leptin levels were increased in 95% of obese and in 66% of overweight subjects. Adiponectin was decreased in 29.5% of obese and in 34% of overweight subjects. An odd ratio analysis revealed a greater probability of increased waist circumference (9.0, systolic blood pressure (4.1, triglyceride (2.3 and insulin (2.9 levels and HOMA-IR (3.0 in the obese group than in the overweight group. The clinical and laboratory parameters and leptin levels exhibited significant correlations, whereas adiponectin was negatively correlated with systolic blood pressure. The occurrence rate of metabolic syndrome was 13.6%. CONCLUSIONS: The high frequency of changes in clinical, laboratory and adipokine markers indicates the need for early interventions aimed at preventing cardiometabolic complications in adulthood.

  12. Oral discoid lupus erythematosus: A study of twenty-one cases

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    Amena M Ranginwala

    2012-01-01

    Full Text Available Aims: This study was undertaken to analyze the histopathological findings of oral discoid lupus erythematosus with conventional light microscopy for early diagnosis of the oral lesions that would aid in prompt treatment. Objectives: To find out the predominant age, sex, site and clinical features of oral discoid lupus erythematosus. To study the histopathological features of oral discoid lupus erythematosus. To study the alterations of basement membrane changes of oral discoid lupus erythematosus. Materials and Methods: Our study consisted 21 cases of diagnosed DLE with oral lesions. A detailed clinical proforma was used for thorough clinical examination and light microscopy was used for histopathological study of the incisional biopsy specimens. Statistical Analysis Used: The lesions were diagnosed on the histopathological criteria given by Gisslen et al. and was statistically analyzed using the Chi square test. Results: In the present study 9.52% patients had only oral lesions, while 90.47% patients had oral lesions along with skin lesions with the most common site of oral involvement being labial mucosa (76.19%, vermillion border (71.42% and buccal mucosa (42.85%. On clinical examination, white spots were present in 28.6%, ulcers in 19% and central erythema in 52.4% lesions. Histopathologically, atrophy was observed in 66.66% cases, acanthosis in 66.66% and acanthosis alternating with atrophy in 33.33% cases along with the basement membrane appearing thin and homogenous in 66.7% and partially destroyed in 81% cases with Periodic Acid Schiff stain. Conclusions: Thus, from this study it was found that a diagnosis of oral discoid lupus erythematosus was based on the combination of clinical and histopathological findings. Thus the dentist may be in an important position to establish the diagnosis with the aid of clinical and histopathological findings before the cutaneous lesions become apparent.

  13. Prediction of esophageal and gastric histology by macroscopic diagnosis during upper endoscopy in pediatric celiac disease

    Science.gov (United States)

    Boschee, Erin D; Yap, Jason Y K; Turner, Justine M

    2017-01-01

    AIM To determine the sensitivity of macroscopic appearance for predicting histological diagnosis at sites other than duodenum in pediatric celiac disease (CD). METHODS Endoscopic and histologic findings in pediatric patients undergoing upper endoscopy for first-time diagnosis of CD at Stollery Children’s Hospital from 2010-2012 were retrospectively reviewed. RESULTS Clinical charts from 140 patients were reviewed. Esophageal and gastric biopsies were taken in 54.3% and 77.9% of patients, respectively. Endoscopic appearance was normal in the esophagus and stomach in 75% and 86.2%. Endoscopic esophageal diagnoses were eosinophilic esophagitis (EE) (11.8%), esophagitis (7.9%), glycogenic acanthosis (1.3%) and non-specific abnormalities (3.9%). Endoscopic gastric diagnoses were gastritis (8.3%), pancreatic rest (0.9%), and non-specific abnormalities (4.6%). Histology was normal in 76.3% of esophageal and 87.2% of gastric specimens. Abnormal esophageal histology was EE (10.5%), esophagitis (10.5%), glycogenic acanthosis (1.3%) and non-specific (1.3%). Gastritis was reported in 12.8% of specimens. Sensitivity and specificity of normal endoscopy for predicting normal esophageal histology was 86.2% and 61.1%, and for normal gastric histology was 87.4% and 21.4%. CONCLUSION In the absence of macroscopic abnormalities, routine esophageal and gastric biopsy during endoscopy for pediatric CD does not identify major pathologies. These findings have cost and time saving implications for clinical practice. PMID:28216971

  14. Spontaneous atopic dermatitis-like symptoms in a/a ma ft/ma ft/J flaky tail mice appear early after birth.

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    Magdalini Kypriotou

    Full Text Available Loss-of-function mutations in human profilaggrin gene have been identified as the cause of ichthyosis vulgaris (IV, and as a major predisposition factor for atopic dermatitis (AD. Similarly, flaky tail (a/a ma ft/ma ft/J mice were described as a model for IV, and shown to be predisposed to eczema. The aim of this study was to correlate the flaky tail mouse phenotype with human IV and AD, in order to dissect early molecular events leading to atopic dermatitis in mice and men, suffering from filaggrin deficiency. Thus, 5-days old flaky tail pups were analyzed histologically, expression of cytokines was measured in skin and signaling pathways were investigated by protein analysis. Human biopsies of IV and AD patients were analyzed histologically and by real time PCR assays. Our data show acanthosis and hyperproliferation in flaky tail epidermis, associated with increased IL1β and thymic stromal lymphopoietin (TSLP expression, and Th2-polarization. Consequently, NFκB and Stat pathways were activated, and IL6 mRNA levels were increased. Further, quantitative analysis of late epidermal differentiation markers revealed increased Small proline-rich protein 2A (Sprr2a synthesis. Th2-polarization and Sprr2a increase may result from high TSLP expression, as shown after analysis of 5-days old K14-TSLP tg mouse skin biopsies. Our findings in the flaky tail mouse correlate with data obtained from patient biopsies of AD, but not IV. We propose that proinflammatory cytokines are responsible for acanthosis in flaky tail epidermis, and together with the Th2-derived cytokines lead to morphological changes. Accordingly, the a/a ma ft/ma ft/J mouse model can be used as an appropriate model to study early AD onset associated with profilaggrin deficiency.

  15. Spontaneous atopic dermatitis-like symptoms in a/a ma ft/ma ft/J flaky tail mice appear early after birth.

    Science.gov (United States)

    Kypriotou, Magdalini; Boéchat, Cloé; Huber, Marcel; Hohl, Daniel

    2013-01-01

    Loss-of-function mutations in human profilaggrin gene have been identified as the cause of ichthyosis vulgaris (IV), and as a major predisposition factor for atopic dermatitis (AD). Similarly, flaky tail (a/a ma ft/ma ft/J) mice were described as a model for IV, and shown to be predisposed to eczema. The aim of this study was to correlate the flaky tail mouse phenotype with human IV and AD, in order to dissect early molecular events leading to atopic dermatitis in mice and men, suffering from filaggrin deficiency. Thus, 5-days old flaky tail pups were analyzed histologically, expression of cytokines was measured in skin and signaling pathways were investigated by protein analysis. Human biopsies of IV and AD patients were analyzed histologically and by real time PCR assays. Our data show acanthosis and hyperproliferation in flaky tail epidermis, associated with increased IL1β and thymic stromal lymphopoietin (TSLP) expression, and Th2-polarization. Consequently, NFκB and Stat pathways were activated, and IL6 mRNA levels were increased. Further, quantitative analysis of late epidermal differentiation markers revealed increased Small proline-rich protein 2A (Sprr2a) synthesis. Th2-polarization and Sprr2a increase may result from high TSLP expression, as shown after analysis of 5-days old K14-TSLP tg mouse skin biopsies. Our findings in the flaky tail mouse correlate with data obtained from patient biopsies of AD, but not IV. We propose that proinflammatory cytokines are responsible for acanthosis in flaky tail epidermis, and together with the Th2-derived cytokines lead to morphological changes. Accordingly, the a/a ma ft/ma ft/J mouse model can be used as an appropriate model to study early AD onset associated with profilaggrin deficiency.

  16. A retrospective study of oral lichen planus in paediatric population

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    Kabita Chatterjee

    2012-01-01

    Full Text Available Aim: Well documented cases of oral lichen planus, a cell mediated immune condition is infrequently reported in paediatric population. This study was undertaken to obtain epidemiological data retrospectively and also to explore the possibility of any association that might exist among the clinical and histopathological features in paediatric patients suffering from oral lichen planus. Subjects and Methods: A retrospective study was carried out on 22 patients, younger than 18 years with clinical and histopathological diagnosis of oral lichen planus over a period of 14 years. The clinical characteristics and histopathological features were observed. The statistical analysis of the data was performed using Statistical Analysis Software (SAS, Version 9.1. Results: Analysis of data of 22 patients revealed that the average age of patient is 15.18 years with equal male and female predilection. The most common site is buccal mucosa (50% and most frequent clinical form is erosive (63.64%. Focusing on the histopathological findings, parakeratosis was found in 86.36% of the cases, acanthosis in 63.64% of cases, moderate basal cell degeneration was identified in 63.64% of cases and dense lymphocytic infiltration at juxtaepithelial connective tissue region was found in 59.09% of cases. Conclusions: Oral lichen planus in paediatric population is rare and appeared between 8 to 18 years of age. There is no significant gender predominance. The most common clinical form is erosive, manifesting mainly in buccal mucosa. Histopathological findings characteristic of oral lichen planus in paediatric patients include parakeratosis, acanthosis, liquefaction degeneration of basal cells and lymphocytic infiltration in the subepithelial layer.

  17. Bat flies (Diptera: Streblidae, Nycteribiidae) parasitic on bats (Mammalia: Chiroptera) at Parque Estadual da Cantareira, São Paulo, Brazil: parasitism rates and host-parasite associations.

    Science.gov (United States)

    Bertola, Patrícia Beloto; Aires, Caroline Cotrim; Favorito, Sandra Elisa; Graciolli, Gustavo; Amaku, Marcos; Pinto-da-Rocha, Ricardo

    2005-02-01

    A total of 443 bat flies belonging to the families Nycteribiidae and Strelidae, were collected on 22 species of bats (Molossidae, Phyllostomidae, and Vespertilionidae) from Parque Estadual da Cantareira (São Paulo, Brazil), between January, 2000 and January, 2001. Eighteen new occurrences of bat flies were recorded on Anoura geoffroyi (Anastrebla caudiferae), Glossophaga soricina (A. caudiferae), Sturnira lilium (Trichobius phyllostomae, T. furmani, and Paraeuctenodes similis), Artibeus lituratus (A. caudiferae), A. fimbriatus (Megistopoda proxima), A. obscurus (Metelasmus pseudopterus), Myotis nigricans (M. proxima, M. aranea, Paratrichobius longicrus), M. ruber (Anatrichobius passosi, Joblingia sp.), M. levis (A. passosi), M. albescens (A. passosi, Basilia andersoni), and Histiotus velatus (M. aranea). Seven new occurrences were recorded for the state of São Paulo, increasing the range for T. tiptoni, T. furmani, M. proxima, Aspidoptera falcata, A. caudiferae, A. modestini and B. andersoni. The relationships between parasitism and host sex, reproductive stage, age hyperparasitism by fungi are discussed.

  18. Presencia de moluscos tropicales de la provincia panameña en la costa central del Perú y su relación con los eventos "El Niño"

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    Carlos Paredes

    2014-06-01

    Full Text Available Se reporta el hallazgo de moluscos cuya distribución conocida se encuentra dentro de los límites de la Provincia Panameña, en diversas localidades de la Provincia Peruana entre los años 1972 y 1994, período en el que se presentaron varios eventos "El Niño". Fue posible determinar 15 especies de moluscos que ampliaron su distribución: Pteria stema, Orobitella peruviana, Cryptornya califomica, Cerithium (T stercusmuscarum, Malea ringens, Hexaplex brassica, Thais styllmanberryi, T (M. triangularis, T (T callaoensis, Acanthina brevidentata, Purpura pansa, Neorapana muricata, Anachis (C. nigricans, Cancellaria (C. decussata y C. (B. chrysostorna. Se postula una variabilidad en los límites de las provincias biogeográficas de la costa peruana, controlada por el ENSO.

  19. Prevalence of dermatologic disorders in 15 patients with acromegaly Prevalência de alterações dermatológicas em 15 pacientes acromegálicos

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    Mariângela Resende

    2012-02-01

    Full Text Available Acromegaly is a chronic, insidious and rare disease, caused by hyper secretion of the growth hormone, which metabolic and trophic effects commonly result in cutaneous manifestations, sometimes preceding other clinical symptoms. The authors have assessed 15 patients with acromegaly and found dermatologic lesions in all of them, mainly skin thickening, acrochordons, epidermoid cysts, pseudoacanthosis nigricans, seborrheic keratosis, melanocytic naevi and lentiginous spots.Acromegalia é doença crônica rara, insidiosa, decorrente da hipersecreção de hormônio do crescimento, cujos efeitos tróficos e metabólicos frequentemente incorrem em manifestações cutâneas, que podem ser precoces. Os autores avaliaram 15 pacientes portadores de acromegalia e evidenciaram alterações dermatológicas em todos, principalmente espessamento da pele, acrocórdons, cistos epidérmicos, pseudoacantose nigricante, queratoses seborreicas, nevos melanocíticos e manchas lentiginosas.

  20. ANTIFUNGAL AND CYTOTOXIC ACTIVITIES OF FIVE TRADITIONALLY USED INDIAN MEDICINAL PLANTS

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    Adhikarimayum Haripyaree

    2013-02-01

    Full Text Available Hexane, Methanol and Distilled water extracts of five Indian Medicinal plants viz., Mimosa pudica L, Vitex trifolia Linn, Leucas aspera Spreng, Centella asiatica (L Urban and Plantago major Linn belonging to different families were subjected to preliminary antimicrobial screening against six standard organisms viz., Ceratocystis paradoxa, Aspergillus niger, Penicillium citrinum, Macrophomina phaseoli, Trichoderma viride and Rhizopus nigricans. To evaluate antifungal activity agar well diffusion method was used. In addition LD50 of the same plant extracts were determined by using Range test on Mus musculus for cytotoxic activity. Methanolic extract of M. pudica showed the highest and significant inhibitory effect against some fungal species. Again, methanolic extract of M. pudica displayed the greatest cytotoxic activity.

  1. Bat flies (Diptera: Streblidae, Nycteribiidae parasitic on bats (Mammalia: Chiroptera at Parque Estadual da Cantareira, São Paulo, Brazil: parasitism rates and host-parasite associations

    Directory of Open Access Journals (Sweden)

    Patrícia Beloto Bertola

    2005-02-01

    Full Text Available A total of 443 bat flies belonging to the families Nycteribiidae and Strelidae, were collected on 22 species of bats (Molossidae, Phyllostomidae, and Vespertilionidae from Parque Estadual da Cantareira (São Paulo, Brazil, between January, 2000 and January, 2001. Eighteen new occurrences of bat flies were recorded on Anoura geoffroyi (Anastrebla caudiferae, Glossophaga soricina (A. caudiferae, Sturnira lilium (Trichobius phyllostomae, T. furmani, and Paraeuctenodes similis, Artibeus lituratus (A. caudiferae, A. fimbriatus (Megistopoda proxima, A. obscurus (Metelasmus pseudopterus, Myotis nigricans (M. proxima, M. aranea, Paratrichobius longicrus, M. ruber (Anatrichobius passosi, Joblingia sp., M. levis (A. passosi, M. albescens (A. passosi, Basilia andersoni, and Histiotus velatus (M. aranea. Seven new occurrences were recorded for the state of São Paulo, increasing the range for T. tiptoni, T. furmani, M. proxima, Aspidoptera falcata, A. caudiferae, A. modestini and B. andersoni. The relationships between parasitism and host sex, reproductive stage, age hyperparasitism by fungi are discussed.

  2. A method for both mass and individual rearing of fungivorous astigmatid mites (Acari).

    Science.gov (United States)

    Okabe, K; Oconnor, B M

    2001-01-01

    Several species of common fungi were assessed as food for fungivorous astigmatid mites. Hypocrea nigricans, Botrytis cinerea and Flammulina velutipes were generally good food sources for most mites examined. Fungal mycelia growing on PDA (potato dextrose agar) medium were not only nutritionally adequate but the system also maintained high humidity through the water-based agar medium. Among acarid mites, most species of Rhizoglyphinae could be reared easily with the method. Although filter-feeding histiostomatid mites do not feed directly on hyphae, some species were successfully maintained with the same method through multiple generations. Presumably, these mites obtained sufficient nutrition from the agar medium and fungal metabolites leaching into it. Most species ultimately produced dispersing heteromorphic deutonymphs on these media. Individual mites were also maintained in isolation within glass rings on fungal colonies. Using this technique, we were able to compare developmental periods, fecundity and survival periods of mites reared under different conditions.

  3. Nuevos registros de Odonata y Ephemeroptera para el noroeste de Argentina

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    José S. RODRÍGUEZ

    2014-01-01

    Full Text Available Se presentan nuevos registros de 14 especies de Odonata y dos especies de Ephemeroptera, incluyendo dos registros nuevos de Argentina: Nephepeltia leonardina Rácenis (Anisoptera: Libellulidae y Alloretochus peruanicus (Soldán (Ephemeroptera: Caenidae. Las distribuciones de Aphylla theodorina (Navás (Anisoptera: Gomphidae y Caenis tenella (Navás (Ephemeroptera: Caenidae se extienden hasta las Yungas (ambas previamente conocidas para el NE de Argentina. También se presentan nuevos registros geográficos de: Neoneura confundens Wasscher & Van’t Bosch (Coenagrionidae; Anax amazili (Burmeister (Aeshnidae; Brachymesia furcata (Hagen, Erythemis plebeja (Burmeister, Erythrodiplax melanorubra Borror, E. nigricans (Rambur, Macrothemis imitans imitans Karsch, M. musiva Calvert, Miathyria marcella (Selys y Micrathyria hesperis Ris (Libellulidae; Progomphus complicatus Selys y Progomphus joergenseni Ris (Gomphidae.

  4. TEMA 11-2016: Síndrome de Ovario Poliquistico

    OpenAIRE

    Borbón Cordero, María Fernanda; Robles Quirós, Fernán

    2016-01-01

    El síndrome de ovario poliquístico afecta 5% a 10% de las mujeres en edad fértil. Es un síndrome heterogéneo caracterizado por anovulación crónica hiperandrogénica, secundaria a una disfunción ovárica intrínseca. Aunque las pacientes afectadas se ven muy perturbadas por las manifestaciones cutáneas, que incluyen acné, hirsutismo, alopecia y acantosis nigricans (1-2), elsíndrome de ovario poliquístico puede tener implicaciones mucho más serias que las encontradas en la piel; con frecuencia cau...

  5. The phytogeographic note on the orchids flora of Vietnam: a case study from the Hon Ba Nature Reserve, Central Vietnam

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    Vuong Ba Truong

    2016-06-01

    Full Text Available The survey of the Orchid flora at Hon Ba Nature Reserve, where was a part of the famous Annamite Range in Vietnam, had been achieved from March to December 2014. A total of 106 species in 4 life forms were accounted i.e. epiphyte (67 species, terrestrial (20 species, lithophyte (13 species and mycoheterotrophic (6 species. Among those, there were two newly recorded species to the Annamite Range i.e. Lecanorchis nigricans Honda and Bulbophyllum dasystachys J.J.Verm., P. Thavipoke & J. Phelps. The most diverse genera were Dendrobium, Bulbophyllum, Liparis and Paphiopedilum respectively. The result area revealed that the Annamite Range performed as a “cross road” of 4 floristic regions in Asia, i.e. the Himalayan Range, the Indo-China Mainland and Myanmar, the Sino-Japanese and the Malesian Region.

  6. Mesure des pertes de croissance radiale sur quelques especes de Pinus dues a deux defoliateurs forestiers. I. Cas de la processionnaire du pin en region mediterraneenne

    OpenAIRE

    Laurent-Hervouet, N.

    1986-01-01

    L’impact des défoliations dues à la processionnaire du pin Thaumetopoea pityocampa Schiff.) sur l’accroissement radial des pins a été étudié grâce à la dendrochronologie au Mont Ventoux sur les pins noirs d’Autriche (Pinus nigra Arn. ssp. nigricans Host.) et dans la vallée du Niolo en Corse sur les pins laricio (Pinus nigra Arn. ssp. laricio Poiret). Les témoins, non attaqués dans les sites étudiés sont constitués par des cèdres (Cedrus atlantica Manetti) au Ventoux et par des pins mari...

  7. Coexistencia de acromegaloidismo y cáncer de pulmón

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    Graciela Stalldecker

    2010-06-01

    Full Text Available El acromegaloidismo es una entidad en la cual se encuentran presentes algunos rasgos acromegálicos sin evidencia bioquímica de hipersecreción de hormona de crecimiento (GH, siendo la resistencia insulínica una de las posibles etiologías. Se presenta un paciente con rasgos acromegálicos, proceso neoplásico en pulmón izquierdo, acantosis nigricans (AN generalizada y marcada resistencia a la insulina (IR. Se descartó acromegalia ante la presencia de una IGF1 normal y GH < 1 ng/ml a los 120 min en el test de tolerancia oral a la glucosa (TTOG. La acantosis nigricans puede presentarse como síndrome paraneoplásico (SPN o puede estar asociada a enfermedad benigna en la cual está involucrada la insulinorresistencia (IR. El acrome-galoidismo está descripto en pacientes con IR grave como el caso que estamos presentando, postulándose que niveles muy elevados de insulina podrían estimular el crecimiento tisular o acral actuando a través del receptor de insulina que conservaría el mecanismo de mediar efectos anabólicos, o alternativamente a través del receptor de IGF1. No encontramos en la literatura la presencia de acromegaloidismo como SPN. En conclusión, presentamos un paciente con IR grave, pseudoacromegalia y AN, coexistiendo con un cáncer de pulmón. Tanto el acromegaloidismo como la AN parecen ser consecuencia de la marcada hiperinsulinemia. Aún así, no podemos descartar que la AN pudiera corresponder a un SPN.

  8. [The study of profile of hypersensitivity to pollen and fungal allergens in the Moscow region].

    Science.gov (United States)

    Akhapkina, I G; Krakhanenkova, S N; Dobronravova, E V; Shushpanova, E N

    2014-05-01

    The profile of hypersensitivity to pollen and fungal allergens is an important element of common pattern of immune diseases needed for development of effective pharmaceuticals. The purpose of the study was to analyze the rate of detection of combined forms of hypersensitivity to pollen and fungal allergens (pollen of birch, hazel, cocksfoot, wormwood, fungi A. alternata, C. herbarum, R. nigricans, P. notatum, C. albicans, A. fumigatus) in the Moscow region on the basis of data of scarification skin samples. The mono-sensibilization was established in 23.36% of all cases of hypersensitivity. At that, among leading allergens turned out A. alternata and cocksfoot pollen (6.54% and 4.67%), followed by allergens of wormwood pollen, P. notatum, R. nigricans, birch pollen and C. albicans (3.74%, 3.74%, 1.87%, 1.87% and 0.93% correspondingly). The polysensibilization was established in 51.40% of cases. Besides, the combined hypersensitivity to pollen allergens of plants (20.26%) and to pollen and fungal allergens (20.56%) occurred more frequently In the group of patients with polysensibilization predominated combined allergic reactions to pollen allergens and A. alternata allergens (36.36%). On the whole, most frequently occurred sensitization to allergens of birch, hazel, cocksfoot, wormwood and A. alternata allergen (76.14%, 69.32%, 57.95%, 55.68%, 39.77% and 56.82% correspondingly). In the Moscow region predominate combined forms of hypersensitivity to two and more pollen and fungal allergens. The polysensitization to pollen allergens and A. alternata allergen occurs more frequently.

  9. Feeding ecology of pelagic fish larvae and juveniles in slope waters of the Gulf of Mexico.

    Science.gov (United States)

    Wells, R J D; Rooker, J R

    2009-11-01

    Stable isotope ratios of carbon (delta13C) and nitrogen (delta15N) were used to investigate feeding patterns of larval and early juvenile pelagic fishes in slope waters of the Gulf of Mexico. Contribution of organic matter supplied to fishes and trophic position within this pelagic food web was estimated in 2007 and 2008 by comparing dietary signatures of the two main producers in this ecosystem: phytoplankton [based on particulate organic matter (POM)] and Sargassum spp. Stable isotope ratios of POM and pelagic Sargassum spp. were significantly different from one another with delta13C values of POM depleted by 3-6 per thousand and delta15N values enriched by 2 relative to Sargassum spp. Stable isotope ratios were significantly different among the five pelagic fishes examined: blue marlin Makaira nigricans, dolphinfish Coryphaena hippurus, pompano dolphinfish Coryphaena equiselis, sailfish Istiophorus platypterus and swordfish Xiphias gladius. Mean delta13C values ranged almost 2 among fishes and were most depleted in I. platypterus. In addition, mean delta15N values ranged 4-5 with highest mean values found for both C. hippurus and C. equiselis and the lowest mean value for M. nigricans during both years. Increasing delta13C or delta15N with standard length suggested that shifts in trophic position and diet occurred during early life for several species examined. Results of a two-source mixing model suggest approximately an equal contribution of organic matter by both sources (POM=55%; pelagic Sargassum spp.=45%) to the early life stages of pelagic fishes examined. Contribution of organic matter, however, varied among species, and sensitivity analyses indicated that organic source estimates changed from 2 to 13% for a delta(13)C fractionation change of +/-0.25 per thousand or a delta15N fractionation change of +/-1.0 per thousand relative to original fractionation values.

  10. Abundância e frugivoria da quiropterofauna (Mammalia, chiroptera de um fragmento no noroeste do Estado do Paraná, Brasil - doi: 10.4025/actascibiolsci.v32i3.5351 Chiropterofauna abundance and frugivory in a forest remnant in northwestern Paraná State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5351

    Directory of Open Access Journals (Sweden)

    Janaina Gazarini

    2010-09-01

    Full Text Available A abundância e a frugivoria de morcegos que compõem a taxocenose em uma área de mata ripária, à margem esquerda do rio Ivaí, foram foco do presente estudo. O Recanto Marista possui 57,6 hectares, dos quais 40,8 são cobertos por Floresta Estacional Semidecidual, situado no município de Doutor Camargo, região Noroeste do Estado do Paraná. Foram realizadas 14 noites de capturas de morcegos de maio de 2007 a janeiro de 2008, com redes-neblina (7 x 2,5 m, totalizando 13.475 m² h de esforço amostral, distribuído em 72h de esforço. Foram capturados 193 indivíduos, representantes de dez espécies, pertencentes a duas famílias: Phyllostomidae (Artibeus lituratus, Sturnira lilium, Carollia perspicillata, Artibeus cf. fimbriatus, Artibeus planirotris, Desmodus rotundus e Pygoderma bilabiatum e Vespertilionidae (Myotis nigricans, Eptesicus sp. e Lasiurus blossevillii. Um representante da família Molossidae (Molossus rufus foi encontrado morto no solo. Foram consumidos frutos pertencentes às famílias Moraceae (Ficus guaranitica, Ficus insipida, Ficus sp. e Maclura tinctoria, Solanaceae (Solanum aspero-lanatum e Solanum sp. , Piperaceae (Piper aduncum, Piper amalago e Piper sp. e Urticaceae (Cecropia pachystachya e Cecropia sp..This study aims to evaluate the abundance and frugivory of bats from the Recanto Marista, a small riparian forest remnant in the margins of the Ivaí river. The Recanto Marista has 57.6 ha, of which 40.8 ha are covered by semideciduous seasonal forest and is located in the Doutor Camargo municipality. Collections were conducted from May 2007 to January 2008 using mist nets (7 x 2.5 m totaling 13,475 m² h and comprising about 72 hours. Ten species were found pertaining to two families, Phyllostomidae (Artibeus lituratus, Sturnira lilium, Carollia perspicillata, Artibeus cf. fimbriatus, Artibeus planirotris, Desmodus rotundus and Pygoderma bilabiatum and Vespertilionidae (Myotis nigricans, Eptesicus sp. and Lasiurus

  11. Inhibition Mould of Distillation Extracts from Seven Species of Natural Plants%小茴香等7种植物蒸馏提取物的抑霉菌效果

    Institute of Scientific and Technical Information of China (English)

    王建清; 杨艳; 金政伟; 赵亚珠

    2011-01-01

    以存在于水果的常见霉菌中的灰霉、黑根霉、交链孢、青霉和黑曲霉为研究对象,采用滤纸片扩散法和时倍稀释法,对小茴香、柠檬草、孜然、广藿香、肉桂叶、桉树叶和鼠尾草这7种植物的蒸馏提取物有效抑菌成分(精油)进行抑菌筛选研究.结果表明:小茴香抑菌效果最好且得率高,为1.90%,对青霉、黑根霉最低抑菌浓度(MIC)均为3.2μL/mL,灰霉、黑曲霉和交链孢MIC为6.4μL/mL;其次为肉桂叶,对交链孢和灰霉有很强抑菌效果,9d内抑菌率为100%.%By filter paper diffusion method and dilution method,the inhibition characters of Foeniculum vulgare Mill,Cymbopogon citratus , Cuminum cyminum L., Pogostemon cablin Benth, Cinnamomum cassia Presl. , Eucalyptus leaves and Salvia japonica Thunb. essential oil extracted by steam distillation were studied. Common Botrytis cinerea,Rhizopus nigricans ,Alternaria.Penicillium citrinum,Aspergillus niger in the mould existing in fruits were chosed as research objects.The results show that Foeniculum vulgare Mill is the most effective bacteriostatic agent and has the highest extraction productivity which is 1.90%. Minimal inhibitory concentration of Foeniculum vulgare Mill effect on Penicillium citrinum and Rhizopus nigricans is 3.2 μL/mL. Minimal inhibitory concentration of Foeniculum vulgare Mill effect on Botrytis cinerea,Aspergillus niger,Alternaria is 6.4 μL/mL. The latter is Cinnamomum cassia Presl,especially to Botrytis cinerea and Alternaria has the best effect. The Inhibitory rate was 100% in 9 d.

  12. Fungi Diversity of Rhizosphere Soil with Loquat Root Rot Disease in Yunnan Province%云南蒙自枇杷根腐病植株根际土壤真菌多样性研究

    Institute of Scientific and Technical Information of China (English)

    杨若鹏; 郑肖兰; 田学军; 李建平; 鲁海菊

    2012-01-01

    摘要摸清蒙自枇杷根腐病病株根际土壤真菌区系分布.为有效可持续防控枇杷根腐病提供理论依据。采用五点取样法采集土壤样品,稀释平板法在PDA培养基上分离纯化,获得114株枇杷根腐病病株根际土壤真菌,经显微镜形态鉴定。109株真菌鉴定为以下24种: A bs idia corymbifera, A bsidia cylindrospora, A bsidia spinosa,Acremonium cerealis, Acremonium murorum, Aspergillus niger, Cladosporium cladosporioides, Colletotrichum dematium, Fusarium flocciferum, Mucor circineUoides f. janssenii, Mucor circinelloides f. wsitanicus, PeniciUium atrovenetum, Penicillium canescens, PeniciUium chrysogenum, Penicillium corylophilum, Penicillium daleae, Penicillium digitatum, Penicillium nigricans, Penicillium oxalicum, Penicillium saceulum, Penicillium variabile,Pythium intermedium, Trichoderma strigosum, Verticillium fungicola,分属于11个属.其中青霉属(Penicillium)是优势属,另外5株真菌属于担子菌。%To establish the species and distribution of fungi of Loquat rhizosphere soil which had rot root disease at Mengzi in Yunnan Province and to provide theoretical basis for efficient control to disease of the root rot; Soil specimen were collected with the 5-point method. The fungi strains were isolated by the plate of gradient dilution with PDA medium. The strains were identified by morphology with microscope. The strains respectively belong to A bsidia corymbifera, A bsidia cylindrospora, A bsidia spinosa, A cremonium cerealis, Acremonium murorum, Aspergillus niger, Cladosporium cladosporioides, Colletotrichum dematium, Fusarium flocciferum, Mucor circinelloides f. janssenii, Mucor circineUoides f. wsitanicus, Penicillium atrovenetum, Penicillium canescens, Penicillium chrysogenum, Penicillium corylophilum, PeniciUium daleae, Penicillium digitatum, Penicillium nigricans, PeniciUium oxalicum, Penicillium saceulum, PeniciUium variabile, Pythium intermedium, Trichoderma strigosum

  13. Abundância e frugivoria da quiropterofauna (Mammalia, chiroptera de um fragmento no noroeste do Estado do Paraná, Brasil = Chiropterofauna abundance and frugivory in a forest remnant in northwestern Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    João Eduardo Cavalcanti Brito

    2010-07-01

    Full Text Available A abundância e a frugivoria de morcegos que compõem a taxocenose em uma área de mata ripária, à margem esquerda do rio Ivaí, foram foco do presente estudo. O Recanto Marista possui 57,6 hectares, dos quais 40,8 são cobertos por Floresta Estacional Semidecidual, situado no município de Doutor Camargo, região Noroeste do Estado do Paraná. Foram realizadas 14 noites de capturas de morcegos de maio de 2007 a janeiro de 2008, com redesneblina (7 x 2,5 m, totalizando 13.475 m² h de esforço amostral, distribuído em 72h de esforço. Foram capturados 193 indivíduos, representantes de dez espécies, pertencentes a duas famílias: Phyllostomidae (Artibeus lituratus, Sturnira lilium, Carollia perspicillata, Artibeus cf. fimbriatus, Artibeus planirotris, Desmodus rotundus e Pygoderma bilabiatum e Vespertilionidae (Myotis nigricans, Eptesicus sp. e Lasiurus blossevillii. Um representante da família Molossidae (Molossus rufus foi encontrado morto no solo. Foram consumidos frutos pertencentes às famílias Moraceae (Ficus guaranitica, Ficus insipida, Ficus sp. e Maclura tinctoria, Solanaceae (Solanum aspero-lanatum e Solanum sp., Piperaceae (Piper aduncum, Piper amalago e Piper sp. e Urticaceae (Cecropia pachystachya e Cecropia sp..This study aims to evaluate the abundance and frugivory of bats from the Recanto Marista, a small riparian forest remnant in the margins of the Ivaí river. The Recanto Marista has 57.6 ha, of which 40.8 ha are covered by semideciduous seasonal forest and is located in the Doutor Camargo municipality. Collections were conducted from May 2007to January 2008 using mist nets (7 x 2.5 m totaling 13,475 m² h and comprising about 72 hours. Ten species were found pertaining to two families, Phyllostomidae (Artibeus lituratus, Sturnira lilium, Carollia perspicillata, Artibeus cf. fimbriatus, Artibeus planirotris, Desmodus rotundus and Pygoderma bilabiatum and Vespertilionidae (Myotis nigricans, Eptesicus sp. and Lasiurus

  14. Morcegos capturados no município de Belo Horizonte, 1999-2003 Bats found in the city of Belo Horizonte, MG, 1999-2003

    Directory of Open Access Journals (Sweden)

    L.V. De Knegt

    2005-10-01

    Full Text Available Entre os anos de 1999 e 2001, realizaram-se 18 capturas de morcegos em Belo Horizonte, sendo uma na estação seca e uma na estação chuvosa em cada regional administrativa. Foram capturados 316 exemplares, com predominância de Artibeus lituratus (Olfers, 1818, 55,1%, Platyrrhinus lineatus (Geoffroy, 1810, 24,4% e Glossophaga soricina (Pallas, 1766, 10,1%, além de Sturnira lilium (Geoffroy, 1810, Phyllostomus discolor (Wagner, 1843, Carollia perspicillata (Linnaeus, 1758, Myotis nigricans (Schinz, 1821 e Anoura caudifer (Geoffroy, 1818, totalizando oito espécies, pertencentes às famílias Phyllostomidae e Vespertilionidae. Observou-se concentração de partos próximos à estação chuvosa, coincidindo com a maior disponibilidade de alimento para os períodos de prenhez e lactação. Partos esporádicos ocorreram durante todo o ano. Entre 2002 e 2003, foi feita uma busca em diversos tipos de abrigos nas mesmas regionais. Os abrigos foram registrados, e as espécies encontradas foram somadas às já observadas na primeira parte deste estudo, bem como às citadas em diversas fontes bibliográficas e disponíveis em coleções acadêmicas, gerando um relato atualizado das espécies de morcegos já encontrados no município.A total of 316 bats were trapped using mist nets during 18 nights at nine strategic locations in Belo Horizonte between 1999 and 2001. The most predominant species were Artibeus lituratus (Olfers, 1818, 55.1%, Platyrrhinus lineatus (Geoffroy, 1810, 24.4%, and Glossophaga soricina (Pallas, 1766, 10.1%. Other trapped species were: Sturnira lilium (Geoffroy, 1810, Phyllostomus discolor (Wagner, 1843, Carollia perspicillata (Linnaeus, 1758, Myotis nigricans (Schinz, 1821 and Anoura caudifer (Geoffroy, 1818, resulting in a total of eight species belonging the families Phyllostomidae and Vespertilionidae. All species bred predominantly during the wet season, probably because of the need for food availability to support energetic

  15. Avaliação do desembarque pesqueiro efetuado em Manacapuru, Amazonas, Brasil Evaluation of the Manacapuru fishing landings, Amazonas State, Brazil

    Directory of Open Access Journals (Sweden)

    Cristiano Gonçalves

    2008-01-01

    Full Text Available A atividade comercial pesqueira na Amazônia Central é predominantemente direcionada para Manaus, porém o perfil das atividades pesqueiras efetuadas nos demais centros também é fundamental para o planejamento do setor. Neste contexto, o presente trabalho visa caracterizar o perfil da produção pesqueira que abastece a cidade de Manacapuru, um dos principais centros urbanos da Amazônia Central. Os desembarques ocorreram a partir de canoas a remo, canoas motorizadas, barcos de pesca e recreios. A média mensal de pescado desembarcado foi de 175,36 ± 39,50 t em 2001 e de 172,13 ± 18,88 t em 2002, não apresentando diferença significativa entre anos (P>0,05. Dos 35 nomes específicos comuns registrados, observa-se que curimatã (Prochilodus nigricans, jaraquis (Semaprochilodus spp., cubiu (Anodus spp., mapará (Hypophthalmus spp., e tambaqui (Colossoma macropomum foram os itens mais importantes nos dois anos, e piramutaba (Brachyplatystoma vaillantii em 2002. Sete sub-regiões foram visitadas pela frota pesqueira, destacando-se Baixo-Solimões e o rio Purus.The fishing commercial activity in Central Amazonia is mainly addressed for Manaus, even so the characteristics of the fishing activities directed to other important urban centers in the region are also fundamental for the planning of the sector. In this context, the present work seeks to characterize the profile of the fishing production that lands in the city of Manacapuru, one of the main urban centers of Central Amazon. Fish landings were done from non-motorized canoes, motorized canoes, fishing ships and pleasure boats. The monthly average of landed fish was of 175,36 ± 39,50 ton in 2001 and of 172,13 ± 18,88 ton in 2002, not presenting significant difference among years (P>0,05. Of the 35 registered common species names, it is observed that curimatã (Prochilodus nigricans, jaraquis (Semaprochilodus spp., cubiu (Anodus spp., mapará (Hypophthalmus spp., and tambaqui (Colossoma

  16. Seasonal and spatial dynamics of a ciliate assemblage in a warm-monomictic Lake Alchichica (Puebla, Mexico Dinámica temporal y espacial de la comunidad de ciliados en un lago monomíctico-cálido Alchichica (Puebla, México

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    Miroslav Macek

    2008-08-01

    Full Text Available The distribution of the ciliate assemblage was surveyed along a depth gradient in the maar crater, athalassohaline, warm monomictic Lake Alchichica (Puebla, Mexico from June 2003 to October 2006 (monthly. DAPI staining was employed to count ciliates while the Quantitative Protargol Staining was used for their identification. Peritrichs often numerically dominated the ciliate assemblage; a maximum of 54 cells ml-1 (Rhabdostyla sp. was observed in the surface layer at the end of the mixing period, during the development of diatoms (Cyclotella alchichicana, cyanobacterial (Nodularia sp. bloom and its decay. Minute spirotrichs (particularly Halteria grandinella and a haptorid, Belonophrya pelagica occasionally dominated the epilimnion while mixotrophic Euplotes cf. daidaleos and Pelagothrix sp. were important round the oxycline along with haptorids, particularly Phialina sp. Scuticociliates Cyclidium glaucoma, Uronema nigricans, and anaerobic ciliates Isocyclidium globosum and Caenomorpha sp. dominated within the hypolimnetic assemblages.De junio de 2003 a diciembre de 2006 se estudió mensualmente la distribución de la comunidad de ciliados en el gradiente de profundidades del Lago Alchichica (Puebla, México, un lago cráter, tipo maar, atalasohalino de comportamiento monomíctico-cálido. La tinción de DAPI fue empleada para el recuento de los ciliados y la tinción de protargol cuantitativa para su identificación. Frecuentemente los ciliados perítricos dominaron numéricamente la comunidad. Al final del periodo de estratificación y durante el desarrollo de las diatomeas (Cyclotella alchichicana y el florecimiento y posterior decaimiento de las cianobacterias filamentosas (Nodularia sp., se observó un máximo de 54 cél. ml-¹ de Rhabdostyla sp. en la superficie del lago. Los espirotricos pequeños (particularmente Halteria grandinella y los haptóridos como Belonophrya pelagica, dominaron ocasionalmente en el epilimnion, mientras que los

  17. A pesca comercial na bacia do rio Madeira no estado de Rondônia, Amazônia brasileira The Commercial fisheries of the Madeira river basin in the Rondônia state, brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Carolina Rodrigues da Costa Doria

    2012-03-01

    Full Text Available Este estudo caracteriza quali e quantitativamente a atividade pesqueira comercial na bacia do rio Madeira, afluente do rio Amazonas, no trecho entre Guajará-Mirim e Porto Velho, estado de Rondônia. No período de janeiro a dezembro/2004, foram registrados 460 t, correspondendo 935 viagens. A análise dos dados oriundos do monitoramento dos desembarques demonstrou que a pesca na região tem caráter artesanal de pequena escala, destacando a maior participação das canoas motorizadas (131 unidades do que barcos pesqueiros (45 unidades; capacidade média: 3.000kg na frota pesqueira. Os peixes migradores jaraqui (Semaprochilodus spp., dourada (Brachyplatystoma rousseauxii, sardinha (Triportheus spp., jatuarana/matrinxã (Brycon amazonicus e B. cephalus, curimatã (Prochilodus nigricans e filhote (Brachyplatystoma filamentosum se destacaram na composição das espécies desembarcadas. As informações técnicas geradas são importantes para subsidiar ações de ordenamento pesqueiro, bem como para avaliar futuras variações que possam ocorrer na atividade frente aos impactos dos empreendimentos hidrelétricos em construção na região.This study presents qualitative and quantitative information about commercial fishery in the basin of the Madeira River, tributary of the Amazon River, describing the fishing activity in the segment between Guajará-Mirim and Porto Velho, in Rondônia State. From January to December/2004, 219 fishermen and 935 trips were registered, corresponding to the capture of 460 t of fish. Data from fish landings demonstrate that fisheries in the region are small-scaled and point to a higher participation of small motorized canoes (130 units than of fishing boats (45 units; average capacity: 3000 kg in the fishing fleet. Migratory species like jaraqui (Semaprochilodus spp., dourada (Brachyplatystoma rousseauxii, sardinha (Triportheus spp., jatuarana/matrinxã (Brycon amazonicus e B. cephalus, curimatã (Prochilodus nigricans

  18. Epidemiología de la diabetes mellitus en la infancia y adolescencia: tipo 1, tipo 2 y ¿diabetes "doble"?

    Directory of Open Access Journals (Sweden)

    Ingrid M. Libman

    2009-09-01

    Full Text Available Durante las últimas décadas, el aporte de la epidemiología a través de la estandarización de los métodos y las definiciones y la colaboración internacional ha permitido una mejor estimación de la incidencia y prevalencia de la diabetes mellitus (DM en la infancia y la adolescencia, así como la detección de probables factores de riesgo para los distintos tipos de la enfermedad, lo que orientaría al desarrollo de medidas preventivas y terapéuticas y la mayor comprensión de la magnitud del problema. Se ha observado un aumento global y amplias diferencias entre distintas regiones en la incidencia de la diabetes tipo 1 (DM1, al igual que un incremento en la frecuencia de la diabetes tipo 2 (DM2 en ciertas etnias asociado a un aumento de la obesidad. Se ha descripto la existencia de la llamada DM "doble" o "híbrida", caracterizada por la asociación de un fenotipo de DM2 (obesidad, acantosis nigricans con manifestaciones de autoinmunidad anti células beta.During the last decades, the contribution of epidemiology through the standardization of methods and definitions and international collaboration allowed a better estimation of the incidence and prevalence of DM in children and adolescents, as well as the detection of likely risk factors for the different types of the disease, which would allow the development of preventive and therapeutic measures and a better understanding of the magnitude of the problem. A global increase and wide differences in the incidence of type 1 diabetes have been observed among regions, as well as an increase in the frequency of type 2 diabetes in some ethnic groups, associated to a growing prevalence of obesity. The existence of what has been named "double" or "hybrid" DM has also been described, characterized by a phenotype of DM2 (obesity and acantosis nigricans associated with manifestations of autoimmunity.

  19. Hubungan Resistensi Insulin dengan Gambaran Klinis Sindrom Ovarium Polikistik

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    Meliza Wahyuni

    2015-09-01

    Full Text Available Abstrak Sindrom Ovarium Polikistik (SOPK merupakan kelainan endokrin dan metabolik pada wanita usia reproduksi. SOPK merupakan kumpulan gejala dari amenore, oligomenore, infertilitas, obesitas, hirsutisme, acne, alopesia, dan akantosis nigrikan. Resistensi insulin diyakini sebagai salah satu penyebab tersering dari SOPK melalui berbagai mekanisme. Tujuan penelitian ini adalah untuk mengetahui hubungan resistensi insulindengan gambaran klinis SOPK. Penelitian ini dilakukan pada pasien SOPK dengan menggunakan studi cross sectional dengan pendekatan retrospektif, yaitu mengumpulkan kejadian masa lalu dari tahun 2009 - 2011, jumlah sampel 105 orang. Analisis statistik yang digunakan adalah uji chi-square. Hasil penelitian didapatkan 33,3% penderita SOPK mengalami resistensi insulin. Berdasarkan gambaran klinis 35,23% amenore, 64,77% oligomenore, 72,04% infertilitas, 50,5% obesitas, 0,95% hirsutisme, acne 20%, alopesia dan akantosis nigrikan0%. Dari 33,3% SOPK dengan resistensi insulin 40% amenore, 60% oligomenore, 71,9% infertilitas, 77,14% obesitas, dan 0% hirsutisme. Berdasarkan hasil uji statistik ditemukan hubungan bermakna antara resistensi insulin dengan obesitas (p<0,05 dan tidak ditemukan hubungan bermakna antara resistensi insulin dengan infertilitas, hirsutisme, dan acne (p>0,05.Kata Kunci: SOPK, resistensi insulin, gambaran klinisAbstract Polycystic Ovary Syndrome (PCOS is an endocrine and metabolic disorders that is common in reproductive-aged women. PCOS is a group of symptoms, such as amenorrhea, oligomenorrhea, infertility, obesity, hirsutism, acne, alopecia, and achanthosis nigricans. Insulin resistance is believed to be one of the most common causes of PCOS through a various mechanisms. The objective of this study was to find out the relationship between insulin resistance and clinical manifestation of PCOS. This research was done in patients with PCOS using cross sectional study with retrospective approach. Data was collected from

  20. Warty Carcinoma Penis: An Uncommon Variant

    Science.gov (United States)

    Ghosh, Arnab; Shrestha, Santosh; Ghartimagar, Dilasma; Narasimhan, Raghavan; Talwar, OP

    2017-01-01

    Penile carcinoma frequency varies widely in different parts of the world and comprises 1–10% of all the malignancies in males. Majority of the cases of penile carcinoma are squamous cell carcinoma of penis comprising 60% to 70% of all cases. Warty carcinoma of penis is an unusual neoplasm and a variant of penile squamous cell carcinoma comprising 5%–10% of all the variants. The other histological variants include basaloid, verrucous, papillary, sarcomatous, mixed, and adenosquamous carcinoma. The various histological entities with an exophytic papillary lesions including warty carcinoma are together referred to as the “verruciform” group of neoplasms. The warty carcinoma has to be differentiated from these lesions and is typically distinguished by histological features of hyperkeratosis, arborescent papillomatosis, acanthosis, and prominent koilocytosis with nuclear pleomorphism. We present a case of 65-year-old male with growth measuring 6 × 4 cm in the penis who underwent total penectomy and was diagnosed as warty carcinoma penis. PMID:28154768

  1. A Case of Nodular Localized Primary Cutaneous Amyloidosis%结节性皮肤淀粉样变性1例

    Institute of Scientific and Technical Information of China (English)

    王晓川; 曹萍; 郝建华

    2012-01-01

    患者女,48岁.右胸部丘疹和结节4年.皮损组织病理示:真皮乳头、网状层、皮下组织及血管周围见大小不一均质性团块样物质沉积,刚果红染色示:橙红色阳性.诊断:结节性皮肤淀粉样变.%A 50-year-old male had been suffered from erythema in his face for 10 years. A verrucous mass developed on his upper lip 3 years ago, and enlarging rapidly in the last 4 months. Biopsy of erythema on his face showed hyperkeratosis, focal parakeratosis, acanthosis, basal cell liquefaction and degeneration and dense dermal infiltration of inflammatory cells. Biopsy of the verrucous mass on the upper lip showed parakeratosis, irregular proliferation and hypertrophy with acantholysis, a large number of vacuolated cells, showing the formation of squa-mous nests and a few dyskeratosis cells, which didn't break through the basement membrane,dermal vascular hyperplasia and massive infiltration of lymphocytes around. The patient was diagnosed as verrucous carcinoma of the upper lip and verruca valgaris on hands developing in discoid lupus erythenmatosus.

  2. Paederus fuscipes dermatitis. A histopathological study.

    Science.gov (United States)

    Borroni, G; Brazzelli, V; Rosso, R; Pavan, M

    1991-10-01

    Paederus fuscipes (PF) dermatitis is a self-healing blistering disorder of the skin caused by a small insect belonging to genus Paederus, family Staphylinidae, order Coleoptera. Crushing PF on the skin causes acute dermatitis within 24 hours, corresponding in shape and dimensions to the area affected by the substance released (pederin). The acute vesicular lesions become crusted and scaly within a few days and heal completely in 10-12 days, with a transitory postinflammatory hypercromic patch. Twenty consecutive cases of PF dermatitis at different stages were studied histologically by routine light microscopy. The pederin causes a spectrum of histopathologic changes ranging from acute epidermal necrosis and blistering in acute stages, to marked acanthosis with mitotic figures in the late stages. PF dermatitis is an entomological model of irritant contact dermatitis, having histopathologic features of intraepidermal and subepidermal blistering, epidermal necrosis and acantholysis. The presence of some acantholytic foci, relatively far from the foci of clinically involved skin, in four of the cases considered suggests a possible role of pederin in inducing acantholysis indirectly. Acantholysis is probably caused by the release of epidermal proteases.

  3. Verrucous Spitz Nevus in a Japanese Female

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    Rie Honda

    2013-11-01

    Full Text Available A 23-year-old female presented with a reddish-brown dome-shaped hyperkeratotic nodule of 11 × 10 mm on the left lower leg. Dermoscopic examination demonstrated a prominent whitish scaly area with ring-like appearance, pinkish-white structureless areas, a few milia-like cysts, dotted and glomerular vessels, and light brown globules. The lesion was completely excised under the diagnosis of verruca vulgaris or dermatofibroma. Histopathological examination revealed a well-circumscribed symmetric lesion with hyperkeratosis, acanthosis, and pseudohorn cysts. The lesion was composed of spindle-shaped and epithelioid melanocytes with large cytoplasm arranged in confluent nests surrounded by stromal fibrosis. Mitotic figures and Kamino bodies were absent. Moderate proliferation of capillaries was found in the papillary dermis. Immunohistochemical staining with melan-A or S-100 was positive for tumor cells, but the staining with HMB-45 was negative. Melan-A staining was weaker in the deeply situated cells than in the superficial ones, which is known as stratification. Finally, we made a diagnosis of verrucous Spitz nevus based on these findings. We should have been aware of the entity of verrucous Spitz nevus as a variant of Spitz nevus and its dermoscopic features in order to reach a correct diagnosis before excision.

  4. Cutaneous Leiomyoma: Novel Histologic Findings for Classification and Diagnosis

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    Kambiz Kamyab Hesari

    2013-01-01

    Full Text Available Smooth muscle tumors rather benign or malignant can arise wherever the muscular tissue presents but cutaneous leiomyoma is one of the rare benign tumors of the which even the diagnostic criteria from the malignant type of the tumor is still in doubt. This study was aimed to compare the subtypes of cutaneous leiomyoma from different histologic aspects in order to find unique criteria for better classification and diagnosis. The six year data base of our center was reviewed and 25 patients with cutaneous leiomyoma were included in this study. Of 25 patients, 5 were female and 20 were male. 5 patients had angioleiomyoma (ALM and 20 had pilar leiomyoma (PLM. ALM had following characteristics: dilated vascular canals intermingled with compact smooth muscle bundles; well circumscribe counter and myxoid and hyaline changes through the tumor. In contrast, PLMs had following histologic features: poor defined outline, entrapped hair follicles and eccrine glands, acanthosis and elongated rete ridges with hyperpigmentation and smooth muscle bundles which are interdigitated with elongated rete ridges. Here we introduced some distinct histological features for each subtype of the cutaneous leiomyoma which can lead to create novel criteria for classification and diagnosis of the lesion.

  5. A Rare Case of Plantar Epithelioma Cuniculatum Arising from a Wart

    Science.gov (United States)

    Ray, Rahul; Bhagat, Aditi; Vasudevan, Biju; Sridhar, Jandhyala; Madan, Renu; Ray, Manjusha

    2015-01-01

    A 68-year-old man, a known case of hypertension, coronary artery disease and old cardiovascular accident with right-sided hemiplegia, came with the chief complaints of a large cauliflower like growth with pus discharge on the left heel since 15 years. The patient had sustained a penetrating injury by a thorn on the left heel region few days before the lesion appeared. Dermatological examination revealed a single verrucous lesion measuring 7 × 7 cm on the left heel region associated with discharge of foul smelling cheesy material. There was also a enlarged right inguinal lymph node which was non-tender, firm, measuring 2 cm in diameter with normal overlying skin. X-ray left ankle was done which showed some soft tissue swelling. A skin biopsy showed hyperkeratosis, acanthosis and parakeratosis. Elongated rete ridges with keratinocyte hyperplasia, forming a large mass pressing on the underlying dermis were seen. There was formation of multiple large keratin filled invaginations and crypts. No atypical cells were seen. Based on history, clinical examination and investigations, a diagnosis of epithelium cuniculatum type of verrucous squamous cell carcinoma was made. A wide excision with a flap cover was performed in consultation with the oncosurgeon and the excision sample was sent for histopathological re-examination, which confirmed the diagnosis of epithelioma cuniculatum. PMID:26538697

  6. Dietary influences of evening primrose and fish oil on the skin of essential fatty acid-deficient guinea pigs.

    Science.gov (United States)

    Chapkin, R S; Ziboh, V A; McCullough, J L

    1987-08-01

    There have been reports that certain dietary lipids are capable of regulating cellular inflammation and hyperproliferation. To investigate further the role of dietary manipulation involving gamma-linolenic acid (18:3n-6) and eicosapentaenoic acid (20:5n-3) on hyperproliferative cellular components, the effects of orally administered primrose oil (containing 18:3n-6) and menhaden fish oil (containing 20:5n-3) were tested in a cutaneous system using the essential fatty acid (EFA)-deficient guinea pig fed a hydrogenated coconut oil (HCO) diet. The effects of the dietary crossover regimen were determined on epidermal 1) morphology, 2) DNA synthesis, 3) delta 6- and delta 5-desaturase activities and 4) fatty acid composition of skin and liver lipids. Our results demonstrated that dietary fish oil lacked the capacity to reverse the signs of epidermal hyperproliferation, acanthosis and hypergranulosis that are characteristic of EFA deficiency. In contrast, primrose oil feeding reversed the histological and biochemical signs of hyperproliferation. These results suggest that dietary fish oil, which contains largely the 20:5n-3 fatty acid, lacks EFA-functional properties in the skin. In addition, substitution of HCO with primrose or fish oil after 6 wk revealed incorporation of 18:3n-6 and 20:5n-3 into epidermal lipids, respectively. The significance of these altered epidermal fatty acid profiles is discussed.

  7. Focal Epithelial Hyperplasia (Heck's Disease) in a 57-Year-Old Brazilian Patient: A Case Report and Literature Review.

    Science.gov (United States)

    de Castro, Luciano Alberto; de Castro, Joao Gabriel Leite; da Cruz, Alexandre Duarte Lopes; Barbosa, Bruno Henrique de Sousa; de Spindula-Filho, Jose Vieira; Costa, Mauricio Barcelos

    2016-04-01

    Focal epithelial hyperplasia (FEH), or Heck's disease, is a rare disease of the oral mucosa associated with infection by some subtypes of human papilloma virus, especially subtypes 13 or 32. The disease is predominantly found in children and adolescents with indigenous heritage, but other ethnic groups can be affected worldwide. To the best of the authors' knowledge, it has not been reported in Brazil's elderly population. This article describes a case of FEH in a 57-year-old Brazilian patient presenting since childhood, with multiple lesions in the lips, buccal mucosa and tongue. The solitary tongue lesion underwent excisional biopsy and the histopathological analysis showed parakeratosis, acanthosis, rete pegs with a club-shaped appearance, koilocytosis and the presence of mitosoid cells. These microscopic findings in conjunction with clinical presentation were sufficient to establish the accurate diagnosis of FEH. Polymerase chain reaction (PCR) was performed, but no one human papillomavirus (HPV) subtype could be identified. Clinicians must be aware of this rare oral disease, which can even affect elderly patients, as we described here. Treatment may be indicated in selected cases due to esthetic and/or functional problems.

  8. Focal Epithelial Hyperplasia (Heck’s Disease) in a 57-Year-Old Brazilian Patient: A Case Report and Literature Review

    Science.gov (United States)

    de Castro, Luciano Alberto; de Castro, Joao Gabriel Leite; da Cruz, Alexandre Duarte Lopes; Barbosa, Bruno Henrique de Sousa; de Spindula-Filho, Jose Vieira; Costa, Mauricio Barcelos

    2016-01-01

    Focal epithelial hyperplasia (FEH), or Heck’s disease, is a rare disease of the oral mucosa associated with infection by some subtypes of human papilloma virus, especially subtypes 13 or 32. The disease is predominantly found in children and adolescents with indigenous heritage, but other ethnic groups can be affected worldwide. To the best of the authors’ knowledge, it has not been reported in Brazil’s elderly population. This article describes a case of FEH in a 57-year-old Brazilian patient presenting since childhood, with multiple lesions in the lips, buccal mucosa and tongue. The solitary tongue lesion underwent excisional biopsy and the histopathological analysis showed parakeratosis, acanthosis, rete pegs with a club-shaped appearance, koilocytosis and the presence of mitosoid cells. These microscopic findings in conjunction with clinical presentation were sufficient to establish the accurate diagnosis of FEH. Polymerase chain reaction (PCR) was performed, but no one human papillomavirus (HPV) subtype could be identified. Clinicians must be aware of this rare oral disease, which can even affect elderly patients, as we described here. Treatment may be indicated in selected cases due to esthetic and/or functional problems. PMID:26985258

  9. Facial porokeratosis.

    Science.gov (United States)

    Carranza, Dafnis C; Haley, Jennifer C; Chiu, Melvin

    2008-01-01

    A 34-year-old man from El Salvador was referred to our clinic with a 10-year history of a pruritic erythematous facial eruption. He reported increased pruritus and scaling of lesions when exposed to the sun. He worked as a construction worker and admitted to frequent sun exposure. Physical examination revealed well-circumscribed erythematous to violaceous papules with raised borders and atrophic centers localized to the nose (Figure 1). He did not have lesions on the arms or legs. He did not report a family history of similar lesions. A biopsy specimen was obtained from the edge of a lesion on the right ala. Histologic examination of the biopsy specimen showed acanthosis of the epidermis with focal invagination of the corneal layer and a homogeneous column of parakeratosis in the center of that layer consistent with a cornoid lamella (Figure 2). Furthermore, the granular layer was absent at the cornoid lamella base. The superficial dermis contained a sparse, perivascular lymphocytic infiltrate. No evidence of dysplasia or malignancy was seen. These findings supported a diagnosis of porokeratosis. The patient underwent a trial of cryotherapy with moderate improvement of the facial lesions.

  10. Inhibition of Keratinocyte Differentiation by the Synergistic Effect of IL-17A, IL-22, IL-1α, TNFα and Oncostatin M

    Science.gov (United States)

    Rabeony, Hanitriniaina; Petit-Paris, Isabelle; Garnier, Julien; Barrault, Christine; Pedretti, Nathalie; Guilloteau, Karline; Jegou, Jean-François; Guillet, Gérard; Huguier, Vincent; Lecron, Jean-Claude; Bernard, François-Xavier; Morel, Franck

    2014-01-01

    Keratinocyte differentiation program leading to an organized epidermis plays a key role in maintaining the first line of defense of the skin. Epidermal integrity is regulated by a tight communication between keratinocytes and leucocytes, particularly under cytokine control. Imbalance of the cytokine network leads to inflammatory diseases such as psoriasis. Our attempt to model skin inflammation showed that the combination of IL-17A, IL-22, IL-1α, OSM and TNFα (Mix M5) synergistically increases chemokine and antimicrobial-peptide expression, recapitulating some features of psoriasis. Other characteristics of psoriasis are acanthosis and down-regulation of keratinocyte differentiation markers. Our aim was to characterize the specific roles of these cytokines on keratinocyte differentiation, and to compare with psoriatic lesion features. All cytokines decrease keratinocyte differentiation markers, but IL-22 and OSM were the most powerful, and the M5 strongly synergized the effects. In addition, IL-22 and OSM induced epidermal hyperplasia in vitro and M5 induced epidermal thickening and decreased differentiation marker expression in a mouse model, as observed in human psoriatic skin lesions. This study highlights the precise role of cytokines in the skin inflammatory response. IL-22 and OSM more specifically drive epidermal hyperplasia and differentiation loss while IL-1α, IL-17A and TNFα were more involved in the activation of innate immunity. PMID:25010647

  11. Acromegaly presenting as hirsuitism: Uncommon sinister aetiology of a common clinical sign

    Directory of Open Access Journals (Sweden)

    Rajesh Jain

    2012-01-01

    Full Text Available Hirsuitism though not uncommon (24%, is not considered to be a prominent feature of acromegaly because of its lack of specificity and occurrence. Hirsuitism is very common in women of reproductive age (5-7% and has been classically associated with polycystic ovarian syndrome (PCOS. Twenty-eight year lady with 3 year duration of hirsuitism (Modified Ferriman Gallwey score-24/36 , features of insulin resistance (acanthosis, subtle features of acromegaloidism (woody nose and bulbous lips was diagnosed to have acromegaly in view of elevated IGF-1 (1344 ng/ml; normal: 116-358 ng/ml, basal (45.1 ng/ml and post glucose growth hormone (39.94 ng/ml and MRI brain showing pituitary macroadenoma. Very high serum androstenedione (>10 ng/ml; normal 0.5-3.5 ng/ml, elevated testosterone (0.91 ng/ml, normal <0.8 and normal dehydroepiandrosterone sulphate (DHEAS (284 mcg/dl, normal 35-430 mcg/dl along with polycystic ovaries on ultrasonography lead to diagnosis of associated PCOS. She was also diagnosed to have diabetes. This case presentation intends to highlight that hirsuitism may rarely be the only prominent feature of acromegaly. A lookout for subtle features of acromegaly in all patients with hirsuitism and going for biochemical evaluation (even at the risk of investigating many patients of insulin resistance and acromegloidism may help us pick up more patients of acromegaly at an earlier stage thus help in reducing disease morbidity.

  12. Skin Barrier Function Is Not Impaired and Kallikrein 7 Gene Polymorphism Is Frequently Observed in Korean X-linked Ichthyosis Patients Diagnosed by Fluorescence in Situ Hybridization and Array Comparative Genomic Hybridization.

    Science.gov (United States)

    Lee, Noo Ri; Yoon, Na Young; Jung, Minyoung; Kim, Ji-Yun; Seo, Seong Jun; Wang, Hye-Young; Lee, Hyeyoung; Sohn, Young Bae; Choi, Eung Ho

    2016-08-01

    X-linked ichthyosis (XLI) is a recessively inherited ichthyosis. Skin barrier function of XLI patients reported in Western countries presented minimally abnormal or normal. Here, we evaluated the skin barrier properties and a skin barrier-related gene mutation in 16 Korean XLI patients who were diagnosed by fluorescence in situ hybridization and array comparative genomic hybridization analysis. Skin barrier properties were measured, cytokine expression levels in the stratum corneum (SC) were evaluated with the tape stripped specimen from skin surface, and a genetic test was done on blood. XLI patients showed significantly lower SC hydration, but normal basal trans-epidermal water loss and skin surface pH as compared to a healthy control group. Histopathology of ichthyosis epidermis showed no acanthosis, and levels of the pro-inflammatory cytokines in the corneal layer did not differ between control and lesional/non-lesional skin of XLI patients. Among the mutations in filaggrin (FLG), kallikrein 7 (KLK7), and SPINK5 genes, the prevalence of KLK7 gene mutations was significantly higher in XLI patients (50%) than in controls (0%), whereas FLG and SPINK5 prevalence was comparable. Korean XLI patients exhibited unimpaired skin barrier function and frequent association with the KLK7 gene polymorphism, which may differentiate them from Western XLI patients.

  13. Clinical studies of pigmented lesions in human skin by using a multiphoton tomograph

    Science.gov (United States)

    Balu, Mihaela; Kelly, Kristen M.; Zachary, Christopher B.; Harris, Ronald M.; Krasieva, Tatiana B.; König, Karsten; Tromberg, Bruce J.

    2013-02-01

    In vivo imaging of pigmented lesions in human skin was performed with a clinical multiphoton microscopy (MPM)-based tomograph (MPTflex, JenLab, Germany). Two-photon excited fluorescence was used for visualizing endogenous fluorophores such as NADH/FAD, keratin, melanin in the epidermal cells and elastin fibers in the dermis. Collagen fibers were imaged by second harmonic generation. Our study involved in vivo imaging of benign melanocytic nevi, atypical nevi and melanoma. The goal of this preliminary study was to identify in vivo the characteristic features and their frequency in pigmented lesions at different stages (benign, atypical and malignant) and to evaluate the ability of in vivo MPM to distinguish atypical nevi from melanoma. Comparison with histopathology was performed for the biopsied lesions. Benign melanocytic nevi were characterized by the presence of nevus cell nests at the epidermal-dermal junction. In atypical nevi, features such as lentiginous hyperplasia, acanthosis and architectural disorder were imaged. Cytological atypia was present in all the melanoma lesions imaged, showing the strongest correlation with malignancy. The MPM images demonstrated very good correlation with corresponding histological images, suggesting that MPM could be a promising tool for in vivo non-invasive pigmented lesion diagnosis, particularly distinguishing atypical nevi from melanoma.

  14. IL-23 induces atopic dermatitis-like inflammation instead of psoriasis-like inflammation in CCR2-deficient mice.

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    Shannon K Bromley

    Full Text Available Psoriasis is an immune-mediated chronic inflammatory skin disease, characterized by epidermal hyperplasia and infiltration of leukocytes into the dermis and epidermis. IL-23 is expressed in psoriatic skin, and IL-23 injected into the skin of mice produces IL-22-dependent dermal inflammation and acanthosis. The chemokine receptor CCR2 has been implicated in the pathogenesis of several inflammatory diseases, including psoriasis. CCR2-positive cells and the CCR2 ligand, CCL2 are abundant in psoriatic lesions. To examine the requirement of CCR2 in the development of IL-23-induced cutaneous inflammation, we injected the ears of wild-type (WT and CCR2-deficient (CCR2(-/- mice with IL-23. CCR2(-/- mice had increased ear swelling and epidermal thickening, which was correlated with increased cutaneous IL-4 levels and increased numbers of eosinophils within the skin. In addition, TSLP, a cytokine known to promote and amplify T helper cell type 2 (Th2 immune responses, was also increased within the inflamed skin of CCR2(-/- mice. Our data suggest that increased levels of TSLP in CCR2(-/- mice may contribute to the propensity of these mice to develop increased Th2-type immune responses.

  15. Surgical management of gingival overgrowth associated with Cowden sydrome: a case report and current understanding.

    Science.gov (United States)

    Feitosa, Daniela da Silva; Santamaria, Mauro Pedrine; Casati, Márcio Zaffalon; Sallum, Enilson Antonio; Nociti Júnior, Francisco Humberto; de Toledo, Sérgio

    2011-05-01

    Cowden syndrome, also known as multiple hamartoma syndrome, is a rare autosomal dominant disorder characterized by multiple hamartomas and a high risk of development of malignancy. Oral findings, such as papillomatous lesions and fibromas, are common features; however, a periodontal phenotype has not been reported previously. Therefore, this report presents a case of gingival overgrowth associated with Cowden syndrome, its successful surgical management, and the 12-month follow-up results. Additionally, we discuss the implications for clinicians. A 23-year-old woman was referred to the Department of Periodontics, Piracicaba Dental School, presenting with generalized gingival overgrowth. A detailed dental and medical history and clinical examination confirmed the systemic diagnosis of Cowden syndrome. Histology, radiographs, and clinical data document the entire clinical approach and follow-up. Clinically, there were minor signs of recurrence of gingival overgrowth in a 12-month period after gingivectomy; however, papular lesions reappeared in keratinized gingiva immediately after healing. No signs of bone loss related to the systemic condition were observed radiographically. Histologically, a dense connective tissue with a moderate chronic inflammatory infiltrate and epithelial acanthosis, which is characteristic of gingival hyperplasia, were demonstrated. Gingival overgrowth may occur as an oral phenotype related to Cowden syndrome and can be successfully treated by means of external bevel gingivectomy, followed by regular maintenance therapy, contributing to the patient's well-being, both functionally and esthetically.

  16. Type 2 diabetes in children: Clinical aspects and risk factors.

    Science.gov (United States)

    Rao, P V

    2015-04-01

    A strong link between obesity, insulin resistance, and metabolic syndrome has been reported with development of a new paradigm to type 2 diabetes mellitus (T2DM), with some evidence suggesting that beta-cell dysfunction is present before the onset of impaired glucose tolerance. Differentiating type 1 diabetes mellitus (T1DM) from T2DM is actually not very easy and there exists a number of overlapping characteristics. The autoantibody frequencies of seven antigens in T1DM patients may turn out to be actually having T2DM patients (pre-T2DM). T2DM patients generally have increased C-peptide levels (may be normal at time of diagnosis), usually no auto-antibodies, strong family history of diabetes, obese and show signs of insulin resistance (hypertension, acanthosis, PCOS). The American Academy of Paediatrics recommends lifestyle modifications ± metformin when blood glucose is 126-200 mg/dL and hemoglobin A1c (HbA1c) 200 mg/dL and HbA1c >8.5, with or without ketosis. Metformin is not recommended if the patient is ketotic, because this increases the risk of lactic acidosis. Metformin is currently the only oral hypoglycemic that has been approved for use in children. Knowing these subtle differences in mechanism, and knowing how to test patients for which mechanism (s) are causing their diabetes mellitus, may help us eventually tailor treatment programs on an individual basis.

  17. Type 2 diabetes in children: Clinical aspects and risk factors

    Directory of Open Access Journals (Sweden)

    P V Rao

    2015-01-01

    Full Text Available A strong link between obesity, insulin resistance, and metabolic syndrome has been reported with development of a new paradigm to type 2 diabetes mellitus (T2DM, with some evidence suggesting that beta-cell dysfunction is present before the onset of impaired glucose tolerance. Differentiating type 1 diabetes mellitus (T1DM from T2DM is actually not very easy and there exists a number of overlapping characteristics. The autoantibody frequencies of seven antigens in T1DM patients may turn out to be actually having T2DM patients (pre-T2DM. T2DM patients generally have increased C-peptide levels (may be normal at time of diagnosis, usually no auto-antibodies, strong family history of diabetes, obese and show signs of insulin resistance (hypertension, acanthosis, PCOS. The American Academy of Paediatrics recommends lifestyle modifications ± metformin when blood glucose is 126-200 mg/dL and hemoglobin A1c (HbA1c 200 mg/dL and HbA1c >8.5, with or without ketosis. Metformin is not recommended if the patient is ketotic, because this increases the risk of lactic acidosis. Metformin is currently the only oral hypoglycemic that has been approved for use in children. Knowing these subtle differences in mechanism, and knowing how to test patients for which mechanism (s are causing their diabetes mellitus, may help us eventually tailor treatment programs on an individual basis.

  18. 阴茎疣状黄瘤1例%A case of Verrucous Xanthoma of Penis

    Institute of Scientific and Technical Information of China (English)

    胡新红; 张衍国; 王欢; 屈慧明

    2012-01-01

    A 50-year-old male's penile foreskin appeared a small ulcer after trauma 1 year ago and scabbed, healed.The local area appeared a small pink papule, growing up to a verrucous isolated goitre which was 1. 0cm X0.5cm, transparent and granular, state clearly, with no discharge. Hislopathological examination showed;epidermis papilloma-like proliferation, parakeratosis, hyperkeratosis, acanthosis, dermal papilla filled with alot of foam cells. Diagnosis; verrucous xanthoma of penis.%患者男,50岁.1年前,阴茎包皮外伤后出现一小溃疡,愈合后局部出现淡红色米粒大丘疹,逐渐增至1.0cm×0 5cm大的淡红色疣状孤立性肿物,呈透明颗粒状,界清,无分泌物.皮损组织病理示:表皮乳头瘤样增生,角化不全,角化过度,棘层肥厚,真皮乳头内见大量泡沫细胞.诊断:疣状黄瘤.

  19. De Novo 17q24.2-q24.3 microdeletion presenting with generalized hypertrichosis terminalis, gingival fibromatous hyperplasia, and distinctive facial features.

    Science.gov (United States)

    Afifi, Hanan H; Fukai, Ryoko; Miyake, Noriko; Gamal El Din, Amina A; Eid, Maha M; Eid, Ola M; Thomas, Manal M; El-Badry, Tarek H; Tosson, Angie M S; Abdel-Salam, Ghada M H; Matsumoto, Naomichi

    2015-10-01

    Generalized hypertrichosis is a feature of several genetic disorders, and the nosology of these entities is still provisional. Recent studies have implicated chromosome 17q24.2-q24.3 microdeletion and the reciprocal microduplication in a very rare form of congenital generalized hypertrichosis terminalis (CGHT) with or without gingival hyperplasia. Here, we report on a 5-year-old Egyptian girl born to consanguineous parents. The girl presented with CGHT and gingival hyperplasia for whom we performed detailed clinical, pathological, and molecular studies. The girl had coarse facies characterized by bilateral epicanthic folds, thick and abundant eyelashes, a broad nose, full cheeks, and lips that constituted the distinctive facial features for this syndrome. Biopsy of the gingiva showed epithelial marked acanthosis and hyperkeratosis with hyperplastic thick collagen bundles and dense fibrosis in the underlying tissues. Array analysis indicated a 17q24.2-q24.3 chromosomal microdeletion. We validated this microdeletion by real-time quantitative PCR and confirmed a perfect co-segregation of the disease phenotype within the family. In summary, this study indicates that 17q24.2-q24.3 microdeletion caused CGHT with gingival hyperplasia and distinctive facies, which should be differentiated from the autosomal recessive type that lacks the distinctive facies.

  20. Phenytoin induced drug rash with eosinophilia and systemic symptoms syndrome: a case report

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    Yogesh Devaraj

    2016-06-01

    Full Text Available DRESS syndrome (drug rash with eosinophilia and systemic symptoms is a severe adverse drug reaction characterised by rash, fever, lymphadenopathy and internal organ involvement. Although the death rate can reach 10%, rapid diagnosis and prompt withdrawal of the offending drug is the key to limit morbidity and mortality. The potential role of corticosteroids remains controversial. We report a case of a 45-year-old male patient who suffered a head injury, for which he was prescribed phenytoin. Five weeks later he developed features of DRESS syndrome including facial and peri-orbital oedema, generalized erythematous, maculo-papular rash, conjunctivitis, inguinal lymphadenopathy, leucocytosis, eosinophilia and elevated liver enzymes. Skin biopsy revealed acanthosis and spongiosis of epidermis and dense inflammatory cell infiltrate comprising eosinophils and lymphocytes. In this case, causalty assessment using Naranjo adverse drug reaction probability scale showed that phenytoin was a probable cause for the adverse drug reaction (score-7. Phenytoin was immediately stopped and patient was treated with systemic corticosteroids. The patient improved dramatically within the next few days. Early recognition of symptoms of DRESS and immediate withdrawal of the offending drug followed by prompt treatment with corticosteroids and other supportive measures will ensure quick recovery and will avoid fatal outcomes. [Int J Basic Clin Pharmacol 2016; 5(3.000: 1148-1151

  1. Gingival pyogenic granuloma developing during isotretinoin treatment

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    Engin Şenel

    2014-12-01

    Full Text Available Pyogenic granuloma (PG is a rare side effect of isotretinoin therapy. Oral pyogenic granuloma developing during isotretinoin treatment has not been reported so far. A 22-old-male patient, who had been given oral isotretinoin treatment for severe nodular acne, was admitted with the complaint of a painless and red nodule in his lower gum at the end of the second month of the treatment. The patient did not report any history of trauma or dental treatment. Dermatologic examination revealed a hemorrhagic nodule measuring 1.5x1 cm in size in the lower gums of the cutting and canine teeth. The lesion was excised completely. Histopathological examination disclosed acanthosis and hyperkeratosis in epidermis, subepidermal vascular proliferation, edema, and sparsely scattered inflammatory cell groups. The mechanism by which isotretinoin causes pyogenic granulomas is not exactly known. It should be considered that this rare side effect can be gingival in patients taking isotretinoin and the regular oral examination should not be neglected.

  2. Demodicose bovina no Estado da Paraíba Bovine demodicosis in the state of Paraíba, northeastern Brazil

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    João Luiz H. Faccini

    2004-09-01

    Full Text Available Casos de demodicose bovina foram observados em um rebanho da raça Sindhi no período de dezembro 1989 a janeiro 1992. Ambas as formas, localizada e generalizada, foram diagnosticadas. Este é o primeiro relato da forma generalizada no Brasil. Nos dois primeiros anos, a demodicose foi diagnosticada somente em animais Cases of bovine demodicosis caused by Demodex bovis were reported in a Sindhi herd from December 1989 to January 1992. Both localized and generalized forms were diagnosed. This is the first report of the generalized form in Brazil. In the first two years, demodicosis was diagnosed only in cattle 2 years old, respectively. Clinical signs varied from a few small nodules to a thickened skin with soft large nodules in the localized and generalized forms, respectively. Main microscopic features of the nodules in the generalized form consisted of acanthosis with hyperqueratosis, chronic sebaceous adenitis, subcutaneous muscular necrosis, focal cellular degeneration of the epidermis basal layer and presence of large number of mites inside the lumen of dilated hair follicles. In addition, a chronic perifoliculitis was observed, characterized by lymphoplasmocytic infiltrate which also contained macrophages and neutrophils. It is suggested that poor nutrition and stress due to prolonged drought probably contributed to the increase of susceptibility of the herd to mite infestation.

  3. Hiperceratose focal acral associada à hipocromia de dermatóglifos Focal acral hyperkeratosis with hypochromic dermatoglyphics

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    Roberto Rheingantz da Cunha Filho

    2008-10-01

    Full Text Available Hiperceratose focal acral é dermatose rara caracterizada por pápulas ceratóticas acrais que afetam preferencialmente as superfícies marginais das mãos e dos pés, pertencendo ao grupo das hiperceratoses marginais. Apresentamos variante inédita de mulher de 45 anos de idade, branca, dona-de-casa, sem história familiar, com lesões ceratóticas papulosas localizadas nos pés e hipocromia de dermatóglifos na região afetada. Histologicamente apresentou hiperortoceratose, desnível da epiderme (degrau, acantose e hipergranulose. Os tratamentos com ceratolíticos tópicos foram ineficientes, de forma semelhante aos casos descritos na literatura atual.Focal acral hyperkeratosis is a rare condition, characterized by acral keratotic papules that preferably affect hand and foot surfaces, and are included in the spectrum of marginal papular keratodermas. We report an unpublished variant of this condition, the case of a 45-year-old Caucasian woman with acral keratotic papular lesions and hypochromic dermatoglyphics in the feet. Histological examination revealed orthohyperkeratosis, depression of the epidermis, acanthosis and hypergranulosis. Topical treatments with keratolytics were ineffective, similarly to previously described cases.

  4. Huriez syndrome with superadded dermatophyte infection

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    Trupti Surana

    2016-01-01

    Full Text Available Palmoplantarkeratodermas (PPKs are a heterogeneous group of hereditary and acquired disorders with underlying gene defects, and characterized by hyperkeratosis of palms and soles with or without other ectodermal and systemic abnormalities. Huriez syndrome is a rare autosomal dominant transgradient type of PPK with high frequency of squamous cell carcinoma in the affected skin. We hereby describe a case of a very rare autosomal dominant PPK in a 40-year-old male patient presenting since birth with PPK extending onto the dorsal aspects of hands and feet with peeling of the skin. The complaints were associated with sclerodactyly, hyperhidrosis, and nail abnormalities. Also superadded dermatophyte infection was observed involving abdomen. No history of loss of any digit. No mucosal, dental, or any systemic involvement was present. No sign of malignancy was noted. Baseline investigations, including ultrasonography of abdomen were normal. Histological findings were nonspecific with only orthohyperkeratosis and acanthosis. Diagnosis was mainly done on clinical grounds. The patient is better with oral retinoids and topical emollients and keratolytics along with antifungal treatment for dermatophyte infection. He is under follow up.

  5. 小汗腺血管瘤样错构瘤1例%A Case of Eccrine Angionmatous Hamartoma

    Institute of Scientific and Technical Information of China (English)

    陈柏叡; 翁立强; 黄卫东

    2013-01-01

    患儿男,3岁2个月.左大腿出现皮疹伴疼痛1年.皮肤科情况:左股伸侧多个丘疹、结节,融合成境界清楚斑块,可见散在毛发分布,按压疼痛明显,无明显多汗.皮损组织病理示:表皮角化过度伴灶性角化不全,棘层呈轻度乳头瘤样增生,真皮浅中层血管成簇、密集增生,深层大量小汗腺腺体和腺管增生.诊断:小汗腺血管瘤样错构瘤.%A three-year and two-month-old boy developed eruptions on the left thigh with pain for 1 year. Several papules and nodules melt into a demarcated plaque in the extensor side of the left thigh, with scattered hairs on, with pain, without hyperhidrosis. Histopathological examination showed hyperkeratosis with focal parakerato-sis and some papillomatous hyperplasia acanthosis in the epidermis; proliferation of clustering capillaries in the middle dermis; increased numbers of large eccrine sweat coils and vessels in the deep dermis. The diagnosis of this patient was eccrine angionmatous hamartoma.

  6. 毛囊角化病1例%A Case of Keratosis Follicularis

    Institute of Scientific and Technical Information of China (English)

    李玉湘; 傅强; 张秀春; 杜丽霞

    2011-01-01

    患者男,38岁,面部及四肢、躯干密集毛囊性丘疹20余年,皮损表现为密集针头及米粒大小褐色毛囊性丘疹,表面油腻性污痂,双侧腹股沟疣状增生.皮损组织病理示:角化过度伴灶状角化不全及角栓形成,棘层增生肥厚,呈疣状增生,表皮上部棘刺松解,可见圆体和谷粒.真皮浅层有少量淋巴细胞为主的炎症浸润.诊断:毛囊角化病.%A 38-year-old male patient presented with needle-size or grain-size and brown follicular papules on the face.trunk and four limbs,greasy crusts on the surface.There was verrucous hyperplasia in the double inguinal grooves.Histopathological exaqmination showed hypergranulosis,spolly parakeratosis and follicular keratotic plugging sphere.acanthosis,papillomatosis and elongation of the rete ridges.There were released of thoms in the upper epidermis and fissure and lacuna could be seen.Superficial perivascular infiltration of lymphocytes in the dermis was observed.This patient was diagnosed as keratosis follicularis.

  7. A Case of Chronic Familial Benign Pemphigus Misdiagnosed as Chronic Eczema%慢性家族性良性天疱疮误诊为慢性湿疹1例

    Institute of Scientific and Technical Information of China (English)

    徐永康; 孙彩虹

    2012-01-01

    A 62-year-old female presented with erythema, papules and plaques and rugae on her trunk and limbs with itching twenty years before. She had been clinically treated as chronic eczema in different hospitals, without obvious improvement hislopathological examination:focal parakeralosis acanthosis. There were focal dyskera-totic cell and mild released of thorns in the epidermis. Perivascular infiltration of focal inflammatory cells in dermis was observed. This patient was diagnosed as familial benign chronic pemphigus.%患者女,62岁.躯干、四肢皮肤皱褶处红斑、丘疹、斑块伴瘙瘁20余年.先后在多家医院均按慢性湿疹治疗,无明显疗效.皮损组织病理示:表皮灶状角化不全,棘层增生肥厚,可见灶状角化不良细胞和轻度的棘刺松解现象.真皮血管周围灶状炎症细胞浸润.诊断:慢性家族性良性天疱疮.

  8. A case of Hypereosinophilic Syndrome Accompanied with Lung Cancer%嗜酸细胞增多性皮炎伴肺癌1例

    Institute of Scientific and Technical Information of China (English)

    宋文婷; 赵广

    2012-01-01

    患者女,60岁,躯干、四肢皮肤干燥、脱屑伴瘙痒1年余,加重1月.近5月出现咳嗽、咳痰,加重伴胸闷、憋气一周.皮肤组织病理:表皮内水肿,棘层肥厚,皮突延长,真皮血管周围见大量嗜酸性粒细胞.胸部CT:肺恶性肿瘤.胸部肿物病理示:低分化腺癌,局灶伴鳞癌分化.诊断:嗜酸细胞增多性皮炎;肺癌.%Female patient, 60 years old, presented with a one-year history of skin dryness and peeling with pruritus on the trunk and four limbs, worsing for one month. In recent 5 months,the patient have been suffered from worsing cough and expectoration for one week accompanied with dyspnea. Pathological examination showed epidermis with dropsy, acanthosis and elongation of the rete ridge. Infiltration of dense eosinophils surrounding of vessels were seen in the dermis. Chest CT showed lung cancer. Histopathology showed poorly differen-tiated adenocareinoma with focal differentiation of squamous cell carcinoma. Diagnosis of eosinophilia dermatitis accompanied with lung cancer was made.

  9. Localized bullous pemphigoid: report of a case with an immunofluorescence and electron microscopical studies on the lesional distribution of 180-KD bullous pemphigoid antigen, beta 4 integrin, and type VII collagen.

    Science.gov (United States)

    Kitajima, Y; Suzuki, M; Johkura, Y; Yaoita, H

    1993-07-01

    A 67-year-old woman with a left-sided hemiplegia had localized bullous pemphigoid demonstrating typical clinical lesions on the left pretibial skin and the radial-side skin of the right forearm. The histology showed a subepidermal blister with extensive hyperkeratosis, hypergranulosis, and acanthosis. Direct immunofluorescence revealed distinct linear deposits of IgG and C3 at the dermo-epidermal junction in the perilesional skin and in the roof of the blisters, but few deposits in nonlesional skin. Electron microscopy revealed separation in the lamina lucida. Indirect immunofluorescence of type VII collagen showed its localization in the blister floor. The distribution of the 180-KD bullous pemphigoid antigen (BPA) and beta 4 integrin, hemidesmosomal transmembrane proteins, were studied in the lesional skin by indirect immunofluorescence. Both 180-KD BPA and beta 4 integrin were localized in the blister roof. By immunoelectron microscopy, beta 4 integrin was detected in small groups on the cell surface facing the blister cavity. Since the epitope of the monoclonal antibody to 180-KD BPA used here is known to be localized at a distance of 20 to 50 nm from the membrane surface and this epitope retained in the blister roof, it appears that the blister was produced in the deep lamina lucida. The lesions were cleared with topical 0.05% clobetasole propionate ointment.

  10. Rhinoentomophthoromycosis due to Conidiobolus coronatus. A case report and an overview of the disease in India.

    Science.gov (United States)

    Chowdhary, Anuradha; Randhawa, H S; Khan, Z U; Ahmad, S; Khanna, G; Gupta, R; Chakravarti, A; Roy, P

    2010-09-01

    Rhinoentomophthoromycosis due to Conidiobolus coronatus is a rare, chronic, granulomatous disease, occurring mainly in tropical Africa, South and Central America and south-east Asia, including India. We report a case of rhinoentomophthoromycosis in a 30-year-old male farmer, a resident of Gorakhpur city in Uttar Pradesh, which was diagnosed by histopathology and isolation C. coronatus in culture. The patient presented with a swollen nose with obstruction that had progressed slowly over one year. His nasal swelling was bilateral, diffuse, mildly tender, erythematous, non-pitting, with mucosal crusting and hypertrophy of inferior turbinates but no regional lympha-denopathy. A contrast-enhanced computed tomography (CECT) scan revealed bilateral pan-sinusitis with nasoethmoid polyposis. Culture of tissue from the nasal biopsy on Sabouraud glucose agar yielded multiple colonies of a mold with satellite smaller colonies at periphery. The isolate demonstrated the macroscopic and microscopic morphologic characteristics of C. coronatus. Its identity was further confirmed by direct DNA sequencing of internal transcribed spacer (ITS) and D1/D2 regions of rDNA. Haemotoxylin and eosin stained tissue sections of the skin biopsy revealed irregular epidermal acanthosis, marked inflammatory and granulomatous reaction with sparse, non-septate hyphae. The patient was treated successfully with a combination therapy of oral saturated potassium iodide solution, itraconazole, and intravenous infusion of amphotericin B. An overview of rhinoentomophthoromycosis cases reported to-date in India is presented.

  11. Pseudoepitheliomatous Hyperplasia Treated by Photodynamic Therapy with Variable Irradiation Dose and Concentration of Photosensitizer

    Science.gov (United States)

    Jiao, Bin; Long, Heather Ann

    2011-01-01

    Abstract Objective: The aim of this study was to test the effectiveness of photodynamic therapy (PDT) in treating pseudoepitheliomatous hyperplasia (PEH) after skin wounding. Background Data: PEH is a difficult-to-treat extreme-degree acanthosis characterized by proliferation of the epithelium. Topical PDT offers an effective and non-invasive treatment for intraepithelial neoplasia and inflammatory dermatosis. These disorders and PEH show the same histological features: epidermal hyperplasia. To our knowledge, there have been no clinical trials published about therapeutic responses of PDT for PEH. Materials and Methods: After application of 10–30% methyl aminolaevulinate (MAL) emulsion, each lesion was irradiated with 633-nm red light at a total dose of 113–339 J/cm2. Therapeutic response was assessed by clinical examination at 3 months. Results: Only 4 of 16 lesions clinically showed a minimal response. No response was observed in 12 of the 16 lesions, either with different cumulative doses or different concentrations of MAL. Conclusion: PEH after skin wounding responds poorly to the topical MAL-PDT. Besides removal of underlying diseases, surgical excision is still the recommended first option. PMID:20969441

  12. Cathepsin L deficiency as molecular defect of furless: hyperproliferation of keratinocytes and pertubation of hair follicle cycling.

    Science.gov (United States)

    Roth, W; Deussing, J; Botchkarev, V A; Pauly-Evers, M; Saftig, P; Hafner, A; Schmidt, P; Schmahl, W; Scherer, J; Anton-Lamprecht, I; Von Figura, K; Paus, R; Peters, C

    2000-10-01

    Lysosomal cysteine proteinases of the papain family are involved in lysosomal bulk proteolysis, major histocompatibility complex class II mediated antigen presentation, prohormone processing, and extracellular matrix remodeling. Cathepsin L (CTSL) is a ubiquitously expressed major representative of the papain-like family of cysteine proteinases. To investigate CTSL in vivo functions, the gene was inactivated by gene targeting in embryonic stem cells. CTSL-deficient mice develop periodic hair loss and epidermal hyperplasia, acanthosis, and hyperkeratosis. The hair loss is due to alterations of hair follicle morphogenesis and cycling, dilatation of hair follicle canals, and disturbed club hair formation. Hyperproliferation of hair follicle epithelial cells and basal epidermal keratinocytes-both of ectodermal origin-are the primary characteristics underlying the mutant phenotype. Pathological inflammatory responses have been excluded as a putative cause of the skin and hair disorder. The phenotype of CTSL-deficient mice is reminiscent of the spontaneous mouse mutant furless (fs). Analyses of the ctsl gene of fs mice revealed a G149R mutation inactivating the proteinase activity. CTSL is the first lysosomal proteinase shown to be essential for epidermal homeostasis and regular hair follicle morphogenesis and cycling.

  13. Histologic classification of penile intraepithelial neoplasia.

    Science.gov (United States)

    Velazquez, Elsa F; Chaux, Alcides; Cubilla, Antonio L

    2012-05-01

    Penile squamous cell carcinomas (SCCs) and their corresponding precancerous lesions can be classified in 2 major groups: human papillomavirus (HPV) related and HPV unrelated. In the former (warty and basaloid SCC), there is a predominance of undifferentiated basaloid cells. In the latter (eg, usual, papillary, and verrucous SCC), the predominant cell is larger with abundant eosinophilic cytoplasm. Based on these morphologic features, a new term, "penile intraepithelial neoplasia" (PeIN), was proposed. PeIN was further subclassified into differentiated and undifferentiated, with the latter being subdivided into basaloid, warty, and warty-basaloid subtypes. Macroscopically, PeIN subtypes are indistinguishable. Microscopically, differentiated PeIN is characterized by acanthosis, parakeratosis, enlarged keratinocytes with abundant "pink" cytoplasm (abnormal maturation), and hyperchromatic cells in the basal layer. In basaloid PeIN the epithelium is replaced by a monotonous population of uniform, small, round, and basophilic cells. Warty PeIN is characterized by a spiky surface, prominent atypical parakeratosis, and pleomorphic koilocytosis. Warty-basaloid PeIN show features of both warty and basaloid PeIN. There is a significant association of subtypes of PeIN with specific variants of invasive SCCs. This is a simple and reproducible nomenclature for penile precancerous lesions based on cell type and differentiation. It takes into account the similarities between vulvar and penile pathology and the hypothesis of a bimodal pathway of penile cancer progression.

  14. New pathologic entities in penile carcinomas: an update of the 2004 world health organization classification.

    Science.gov (United States)

    Chaux, Alcides; Velazquez, Elsa F; Barreto, José E; Ayala, Enrique; Cubilla, Antonio L

    2012-05-01

    Most primary malignant tumors of the penis are squamous cell carcinomas (SCC) of the usual type. In recent years several variants, each with distinctive clinicopathologic features, have been described. Pseudohyperplastic carcinoma and carcinoma cuniculatum are both low-grade, extremely well-differentiated SCC variants characterized by an indolent clinical course and good prognosis. The former, which may be confused with pseudoepitheliomatous hyperplasia, preferentially affects the inner foreskin mucosa of elderly men and the latter is a verruciform tumor with an endophytic, burrow-like pattern of growth. Pseudoglandular carcinoma (featuring solid tumor nests with extensive central acantholysis simulating glandular lumina) and clear cell carcinoma (human papillomavirus [HPV]-related tumors composed of periodic acid-Schiff positive clear cells) are aggressive tumors with a high incidence of inguinal nodal metastases. Papillary carcinomas are HPV-unrelated verruciform tumors composed of complex papillae with acanthosis, hyper- and parakeratosis, absence of koilocytes, irregular fibrovascular cores, and jagged tumor base. Finally, in warty-basaloid carcinomas areas of warty (condylomatous) and basaloid carcinomas coexist in the same tumor, either separated or intermingled, giving the tumor a variegated appearance. In this review special emphasis is given to the differential diagnosis of these special variants with a discussion of the possible implications for clinical management.

  15. A Man with an Umbilicated Papule of the Hand: What Is Your Diagnosis?

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    Deba P. Sarma

    2010-01-01

    Full Text Available Introduction. Ecthyma contagiosum is a zoonotic disease caused by the parapoxvirus that causes “sore mouth” in sheep and goats and orf in human. Case Presentation. A 61-year-old sheep farmer presented with a painful non-pruritic lesion on the left hand that had been present for approximately 5 weeks. Physical examination demonstrated a 1 cm pearly, umbilicated papule with raised borders. A biopsy showed an asymmetrical nodule with parakeratotic crust and acanthosis with thin epidermal strands extending deeply in the underlying dermis. Marked edema, capillary proliferation and extensive lymphocytic infiltration was also present. One red intranuclear inclusion was identified in an epidermal keratinocyte. A diagnosis of human orf (ecthyma contagiosum was made. Conclusion. Infected sheep and freshly vaccinated sheep or goats are the reservoir for human infection. After an incubation period of 3–7 days, parapoxvirus infections produce 1–3 painful lesions measuring 1-2 cm in diameter. The natural history of the disease is complete resolution and no treatment is indicated. Prevention of echthyma contagiosum in ruminants through vaccination is thought to be the best way to control infection.

  16. Assessment of dermal exposure and histopathologic changes of different sized nano-silver in healthy adult rabbits

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    Koohi, Mohammad kazem; Hejazy, Marzie [Toxicology division, Basic science department, Faculty of Veterinary Medicine, University of Tehran, Qareeb Street, Azadi Av. PO Box: 14155-6453, Tehran (Iran, Islamic Republic of); Asadi, Farzad [Biochemistry division, Basic science department, Faculty of Veterinary Medicine, University of Tehran, Qareeb Street, Azadi Av. PO Box: 14155-6453, Tehran (Iran, Islamic Republic of); Asadian, Peyman, E-mail: mkkoohi@ut.ac.ir [Clinical pathology department, Faculty of Veterinary Medicine, University of Khorramabad (Iran, Islamic Republic of)

    2011-07-06

    The purpose of this study is to evaluate the dermal toxicity (Irritation/Corrosion) of three sizes of nanosilver particles (10, 20 and 30 nm) during 3 min, 1 and 4 hours according to the OECD/OCDE guideline Histopathological effects in secondary organs from liver, kidney, heart, spleen and brain 14 day post dermal administration are also reported. 10 and 20 nm Ag nanoparticles treated group showed well defined dermal erythema and oedema. Histopathological findings of 10 and 20 nm (4 hours exposure) on 14-day post dermal administration showed hyperkeratosis, acanthosis, hair-filled follicles and papillomatosis in an irregular epidermis, fibrosis, hyperemia, erythema, intracellular oedema and hyalinisation of collagen in dermis of skin. Liver revealed midzonal and periacinar necrosis, portal mononuclear infiltration, liver fatty change, liver congestion and hyperemic central vein. Splenic red pulp congestion and white pulp hyperreactivity, splenic trabeculae and sinusoidal congestion and hyaline change were found in spleen. Fatty degeneration in some cardiovascular cells and subendocardial hemorrhage without inflammation was perceived. Picnotic appearance of pyramidal neurons in the brain cortex, gliosis and mild perineuronal oedema ischemic cell change and hyperemic meninges was observed in brain. Our research concluded that dermal exposure to lesser sizes of silver nanoparticles is more disastrous than greater ones.

  17. LIKEN PLANUS

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    Herman Cipto

    2015-08-01

    Full Text Available Lichen planus (LP is an inflammation skin and mucous membrane disease that has characteristic clinical and pathologic features. Typical lesion of LP are small, flat-topped, polygonal, violaceous papules on the skin or reticular, white patch or plaque on mucous membrane. Nail involvement is manifested by longitudinal ridging, thinning and distal splitting and hair involvement is atrophic cicatrical alopesia. There was an increased risk of developing squamous cell carcinoma in the leasion of mucous membrane of oral cavity. Pathology of LP is the prototype of lichenoid interface dermatitis, which shows damage to the basal cell layer and a band-like dental lymphocytic infiltrate in close approximation to the epidermis. Other pathologic findings are compact orthokeratosis, wedge-shape hypergranulosis and irregular acanthosis. The treatment of LP in the main is largely symptomatic like antipruritic for itching. Therapies to suppress immune responses are commonly effective in treating LP, such as corticosteroid and cyclosporine topical or systemic. Other treatment are psoralene ultra violet A (PUVA or steroids.

  18. A clinico-pathological study of 70 cases of pemphigus

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    Arya S

    1999-01-01

    Full Text Available A clinicopalhological study of 70 cases of pemphigus observed over a span of four and a half years from January 1992 to June 1996 at the Sir J.J. Group of Hospitals and Grant Medical College, Mumbai is reported. Pemphigus vulgaris constituted the single largest group of 43 cases, followed by pemphigus foliaceus (25 cases and pemphigus vegetans (2 cases. Majority of the cases were seen in the age group of 21-60 years, with a slight male predominance. The youngest patient was 14 years while the eldest was aged 75 years. Mucosal involvement was seen in 31 cases of pemphigus vulgaris, as opposed to only 5 cases of pemphigus foliaceus. Flaccid bullae were present in 100% cases. Pruritus was complained of in 14 cases, though it was more common in pemphigus vegetans and vulgaris. Salient histopathological features of pemphigus vulgaris observed were (I intraepidermal suprabasal blisters (35 cases, (2 presence of acantholytic cells (40 cases, (3 "Row of tombstone appearance" (I8 cases and (4 acantholysis involving follicular sheath (20 cases. Main histopathological features of pemphigus foliaceus were (1 subcorneal blister (15 case, (2 acantholysis (24 cases and (3 bulla cavity containing inflammatory infiltrate (12 cases. Both cases of pemphigus vegetans showed hyperkeratosis, papillomatosis and irregular acanthosis with intra-epidermal eosinophilic abscesses besides suprabasal lacunae.

  19. Buschke-Löwenstein Tumour: Successful Treatment with Minimally Invasive Techniques

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    Estefânia Correia

    2015-01-01

    Full Text Available We report a case of an 80-year-old female who presented with a four-year history of a growing mass in the perianal area with pain and bleeding during defaecation. Clinical examination revealed a locally destructive, cauliflower-like, verrucous mass measuring 10 × 12 cm in diameter. Histologic findings revealed a moderate degree of dysplasia of the epithelium with koilocytosis atypia, acanthosis, and parakeratosis, features that are consistent with Buschke-Löwenstein tumour. Polymerase-chain-reaction assay for human papillomavirus (HPV showed an infection with HPV type 11. Full-thickness excision of involved skin was undertaken by cryotherapy and electrocautery over five months. The entire wound was left open to heal by secondary intention. After 3 years of follow-up, the patient has not experienced a recurrence, with excellent functional results, but the cosmetic results were satisfactory. These minimally invasive techniques can be safer and more cost-effective than surgery and the General Practitioner can play a key role in diagnosis.

  20. Caso para diagnóstico Case for diagnosis

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    Patricia Erica Christofoletti Daldon

    2010-10-01

    Full Text Available Paciente do sexo masculino, negro, 13 anos, apresenta há dois anos lesões pruriginosas, pápulonodulares nos antebraços, associadas a edema do lábio inferior, fotofobia, conjuntivite e pterígio. O exame histopatológico do lábio inferior revelou acantose, espongiose e infiltrado inflamatório perivascular superficial, composto por linfócitos, plasmócitos e eosinófilos, compatível com o diagnóstico de prurigo actínico. As lesões regrediram com o uso de talidomida 100 mg/dia.A 13-year-old black boy had pruritic papular and nodular lesions on his forearms associated to edema of the lower lip, photophobia, conjunctivitis and pterygium. Skin biopsy of the lower lip revealed acanthosis, spongiosis with dermal perivascular mononuclear cell infiltration composed by lymphocytes, plasma cells and eosinophils consistent with actinic prurigo. Lesions improved considerably with the use of thalidomide 100mg/ day.

  1. Mercury Bioaccumulation Response to Recent Hg Pollution Abatement in an Oceanic Predatory Fish, Blue Marlin, Versus the Response in a Coastal Predatory Species, Bluefish, in the Western North Atlantic Ocean

    Science.gov (United States)

    Barber, R. T.; Cross, F. A.

    2015-12-01

    The consumption of marine fish, especially predatory species high in the food chain, is the major route through which people in developed countries are exposed to mercury. Recent work on a coastal species, bluefish (Pomatomus saltatrix), determined that the mercury concentration in fish from the U. S. Mid-Atlantic coast decreased 43% from 1972 to 2011. This mercury decline in a coastal marine fish parallels the mercury decline in many freshwater fish in the U.S. and Canada during the same time period. The result heightens interest in determining whether or not there has been any change in mercury concentration in oceanic predatory fish species, that is, fish that are permanent residents of the open ocean, during the past four decades. To answer this question we compared mercury analyses we made in the 1970s on tournament-caught blue marlin (Makaira nigricans) with those we made from 1998 to 2013. This comparison indicates that from the 1970s to 2013 mercury concentration in blue marlin caught in the western North Atlantic Ocean off the U.S. east coast has declined about 45%, a decline that is remarkably similar to the decline reported in coastal bluefish. These results suggest that a large area of the western North Atlantic Ocean is responding to reductions in emissions of mercury in the U.S. and Canada with reduced mercury bioaccumulation in predatory fish.

  2. Anxiolytic effect of the homeopathic complex Tepeex®

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    Ezequiel Paulo Viriato

    2009-09-01

    Full Text Available Aims: Homeopathic complex Tepeex® is a compound of Actaea racemosa 4cH, Natrum muriaticum 2cH, Pulsatilla nigricans 3cH, Chamomilla 3cH and Sepia succus5cH. This study evaluated the effect of Tepeex® in pre-clinical models of depression and anxiety. Methods: the following tests were performed: elevated plus maze test (EPM; forced swimming test (FST; open field test (OFT and Rotarod test (RRT. Results: In EPM, animals treated with Tepeex® on days 20 and 30 stayed longer in the open arms of the maze than the control group (p < 0.05, Dunnett test. In FST, treatment with Tepeex® did not increase swimming time compared to the control group in any phase of treatment. In OFT, crossing increased significantly with treatment with amfepramone, and also with 30-day treatment with Tepeex® (p < 0.05, Dunnette test. In RRT, treatment with amfepramone significantly reduced latency time. 30-day treatment with Tepeex® did not affect motor coordination when compared to the control group. Conclusion: results suggest that homeopathic complex Tepeex® has anxiolytic properties without affecting motor coordination.

  3. Mass dynamics of wintering Pacific Black Brant: Body, adipose tissue, organ, and muscle masses vary with location

    Science.gov (United States)

    Mason, D.D.; Barboza, P.S.; Ward, D.H.

    2007-01-01

    We compared body size and mass of the whole body, organs, adipose tissue, and muscles of adult Pacific Black Brant (Branta bernicla nigricans (Lawrence, 1846)) collected concurrently in Alaska and Baja California during the fall, winter, and spring of 2002–2003. Head and tarsal lengths of males were similar between sites and slightly larger for females in Alaska than in Baja California. Brant appear to operate under similar physiological bounds, but patterns of nutrient allocation differ between sites. Birds wintering in Alaska lost similar amounts of adipose tissue during early winter as birds in Baja California gained during late winter before migration. Masses of the body, adipose tissue, and flight muscles during mid-winter were similar between sites. Seasonal adipose tissue deposition may, therefore, equally favor winter residency or long-distance migration. Gonad and liver masses increased in late winter for birds in Alaska but not for those in Baja California, suggesting birds wintering in Baja may delay reproductive development in favor of allocating reserves needed for migration. Phenotypic flexibility allows Brant to use widely divergent wintering sites. The wintering location of Brant likely depends more upon changes in environmental conditions and food availability, than upon physiological differences between the two wintering populations.

  4. Yield of Fumaric Acid from Different Carbohydrates by Stationary Surface Culture Method

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    Km. Maya Saijwani

    1968-07-01

    Full Text Available Studies on the yield of fumaric acid by Rhizopus arrhizus and Bhizopus nigricans using glucose, sucrose, xylose,cane molassess and bagasse hydrolysate either alone or in combination with glucose were undertaken. Thirty three and 26 per cently yield of fumaric acid were obtained from 12 and 10 per cent glucose media respectively, in 15 days at 33 degree calcius by stationary surface culture method. Higher yields of fumaric acid were obtained from glucose using R. arrhizus. Ability of this organism to produce fumaric acid from media containing (i 50:50 glucose and deionized bagasse,hydrolysate (ii deionized bagasse hydrolysate, and (iii clarified cane molasses was investigated. A 7 per cent yield of fumaric acid was obtained from 50:50 glucose and deionized bagasse hydrolysate medium. In the case of xylose good growth of the fungus was obtained but no fumaric acid was produced. These studies have indicated that R. arrhizus is not able to utilize pentose sugars.

  5. Bioremediation of aflatoxins by some reference fungal strains.

    Science.gov (United States)

    El-Shiekh, Hussein H; Mahdy, Hesham M; El-Aaser, Mahmoud M

    2007-01-01

    Aspergillus parasiticus RCMB 002001 (2) producing four types of aflatoxins B1, B2, G1, and G2 was used in this study as an aflatoxin-producer. Penicillium griseofulvum, P. urticae, Paecilomyces lilacinus, Trichoderma viride, Candida utilis, Saccharomyces cerevisiae as well as a non-toxigenic strain of Aspergillus flavus were found to be able to exhibit growth on aflatoxin B1-containing medium up to a concentration of 500 ppb. It was also found that several fungal strains exhibited the growth in co-culture with A. parasiticus, natural aflatoxins producer, and were able to decreased the total aflatoxin concentration, resulting in the highest inhibition percentage of 67.2% by T viride, followed by P. lilacinus, P. griseofulvum, S. cerevisiae, C. utilis, P. urticae, Rhizopus nigricans and Mucor rouxii with total aflatoxin inhibition percentage of 53.9, 52.4, 52, 51.7, 44, 38.2 and 35.4%, respectively. The separation of bioremediation products using GC/MS revealed that the toxins were degraded into furan moieties.

  6. An optimization study of solid-state fermentation: xanthophylls extraction from marigold flowers.

    Science.gov (United States)

    Luis, Navarrete-Bolaños José; Hugo, Jiménez-Islas; Enrique, Botello-Alvarez; Ramiro, Rico-Martínez; Octavio, Paredes-López

    2004-09-01

    Marigold flowers are the main natural source of xanthophylls, and marigold saponified extract is used as an additive in several food and pharmaceutical industries. In this work, the use of a solid-state fermentation (ensilage) process for increasing the yield of xanthophylls extracted from fermented marigold flowers was examined. The process consisted of a mixed culture of three microorganisms (Flavobacterium IIb, Acinetobacter anitratus, and Rhizopus nigricans), part of the normal microbiota associated with the marigold flower. These microorganisms had been previously isolated, and were identified as relevant for the ensilage process due to their capacity to produce cellulolytic enzymes. Based on experimental design strategies, optimum operation values were determined for aeration, moisture, agitation, and marigold-to-inoculum ratio in the proposed solid-state fermentation equipment, leading to a xanthophylls yield of 17.8-g/kg dry weight. The optimum achieved represents a 65% increase with respect to the control. HPLC analysis indicated conservation of extracted oleoresin. Based on the experimental results, interactions were identified that could be associated with the heat and mass-transfer reactions taking place within the bioreactor. The insight gained allows conditions that limit growth and metabolic activity to be avoided.

  7. 海南省菠萝蜜主要病虫害识别与防治%Control of Jackfruit Main Pests and Diseases in Hainan Province

    Institute of Scientific and Technical Information of China (English)

    刘爱勤; 桑利伟; 孙世伟; 谭乐和; 苟亚峰; 吴刚

    2012-01-01

    分别介绍了目前在海南省菠萝蜜上发生的6种主要病虫害,炭疽病、花果软腐病、蒂腐病和榕八星天牛、桑粒肩天牛及黄翅绢野螟,以及6种病虫害的症状、病原或害虫、发生规律及防治措施,为促进菠萝蜜产业健康可持续发展提供技术参考。%There were six kinds of Jackfruit serious diseases that included jackfruit anthracnose, jackfruit Diplodia artocarp rot, jackfi'uit Rhizopus nigricans fi'uit rot, jackfi'uit Batocera rubus L., jackfruit Apriona germari Hope and jackfruit D. caescdis Walker. In this paper the symptom, pathogen, occurring rule and control measures were introduced to these diseases.

  8. Species richness and abundance of bats in fragments of the stational semidecidual forest, Upper Paraná River, southern Brazil

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    H. Ortêncio-Filho

    Full Text Available The Upper Paraná River floodplain is inserted in a region of the Mata Atlântica biome, which is a critical area to preserve. Due to the scarcity of researches about the chiropterofauna in this region, the present study investigated species richness and abundance of bats in remnants from the stational semidecidual forest of the Upper Paraná River, southern Brazil. Samplings were taken every month, from January to December 2006, using 32 mist nets with 8.0 x 2.5 m, resulting in 640 m²/h and totaling a capture effort of 87,040 m²/h. In order to estimate the species richness, the following estimators were employed Chao1 and Jack2. During the study, a total of 563 individuals belonging to 17 species (Artibeus planirostris, Artibeus lituratus, Carollia perspicillata, Platyrrhinus lineatus, Sturnira lilium, Artibeus fimbriatus, Myotis nigricans, Desmodus rotundus, Artibeus obscurus, Noctilio albiventris, Phylostomus discolor, Phylostomus hastatus, Chrotopterus auritus, Lasiurus ega, Chiroderma villosum, Pygoderma bilabiatum and Lasiurus blossevillii were captured. The estimated richness curves tended to stabilize, indicating that most of the species were sampled. Captured species represented 10% of the taxa recorded in Brazil and 28% in Paraná State, revealing the importance of this area for the diversity of bats. These findings indicate the need to determine actions aiming to restrict human activities in these forest fragments, in order to minimize anthropogenic impacts on the chiropterofauna.

  9. Structure of a bat assemblage (Mammalia, Chiroptera in Serra do Caraça Reserve, South-east Brazil

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    Falcão Fábio de C.

    2003-01-01

    Full Text Available The Serra do Caraça Reserve is situated in the southern portion of the Espinhaço Mountain Range, and contains areas of "campos de altitude", "cerrado" and atlantic forest. This study had as its objective the registering of the bats species that occur in the reserve. The data collection was carried out in one year through monthly samplings, using mist nets set on trails, and also through hand capture. A total of 246 individuals were collected (0.72 bats/net-hour, distributed across 15 species, belonging to the families Phyllostomidae (83.0%; nine species, Vespertilionidae (12.5%; three species and Molossidae (4.5%; three species. The most abundant species were Sturnira lilium (E. Geoffroy, 1810 (n = 121, 60.5%, Myotis nigricans (Schinz, 1821 (n = 21, 10.5% and Platyrrhinus lineatus (E. Geoffroy, 1810 (n = 10, 5.0%, and less represented were Lasiurus blossevilli (Lesson y Garnot, 1826 (n = 2, 1.0%, Eumops perotis (Schinz, 1821 (n = 2, 1.0% e Vampyressa pusilla (Wagner, 1843 (n = 1, 0.5%. The richness of species found and the non-occurrence of phyllostomines in the reserve could be indicative of some level of forest disturbance.

  10. Annotated check list of the Pyraloidea (Lepidoptera) of America North of Mexico.

    Science.gov (United States)

    Scholtens, Brian G; Solis, M Alma

    2015-01-01

    An annotated check list of Pyraloidea of North America north of Mexico is presented, including 861 Crambidae and 681 Pyralidae with 1542 total species. It includes all new species described, tropical species with new records in the United States, and species introduced from Europe and Asia since 1983. The Notes section provides the seminal citations, data and/or commentary to all changes since 1983 for easy and future reference. In addition, this list proposes seven new generic combinations, the transfer of a phycitine species, Salebria nigricans (Hulst), to Epipaschiinae and its syn. n. with Pococera fuscolotella (Ragonot), and three new records for the United States. Purposefully, no new taxa are described here, but we found a gradual increase of 10% in the number of species described since 1983. Finally, we also include a list of thirteen species not included or removed from the MONA list. Many higher-level changes have occurred since 1983 and the classification is updated to reflect research over the last 30 years, including exclusion of Thyrididae and Hyblaeidae from the superfamily and recognition of Crambidae and Pyralidae as separate families. The list includes multiple changes to subfamilies based on morphology such as the synonymization of the Dichogamini with the Glaphyriinae, but also incorporating recent molecular phylogenetic results such as the synonymization of the Evergestinae with the Glaphyriinae.

  11. [7alpha-hydroxylation of steroid 5-olefins by mold fungi].

    Science.gov (United States)

    Andriushina, V G; Druzhinina, A V; Iaderets, V V; Stytsenko, T S; Voĭshvillo, N E

    2010-01-01

    Hydroxylation activity of the mold fungi belonging to the orders Dothideales, Hypocreales, and Mucorales towards delta(5)-3beta-hydroxysteroids was studied. The fungi Bipolaris sorokiniana, Fusarium sp., and Rhizopus nigricans were able to introduce hydroxy group at position 7alpha; however, this ability was detected only at a low substrate load and with a low yield. A 7alpha-hydroxylase activity of the Curvularia lunata VKPM F-981 culture was shown for the first time. It was demonstrated that the studied strain was capable of stereo- and regioselective transformations of androstane 5-olefins at a load not less than 2 g/l. Conversion of pregnane steroids by this culture yielded both 7alpha and 11beta-hydroxy derivatives. The introduction of 7alpha-hydroxy group by this strain occurred concurrently with enzymatic hydrolysis of ester groups, which proceeded under mild conditions to give the corresponding alcohols in the cases of both 3-acetate of delta(5)-androstenes and mono- and triacetates of delta(5)-pregnenes.

  12. Colony size, sex ratio and cohabitation in roosts of Phyllostomus hastatus (Pallas (Chiroptera: Phyllostomidae

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    LM. Costa

    Full Text Available Phyllostomus hastatus bat is species broadly distributed over the Neotropical region, which uses as diurnal roosts caves, hollow trees, palm leaves and human buildings. Thirteen diurnal roosts of P. hastatus were analysed from 1990 to 2009 in several localities of Rio de Janeiro State, regarding environment (rural, urban or protected area, type of roost (hollow tree, basement or roof, sex ratio and cohabitation. A nocturnal roost was also analysed. Sex ratio of P. hastatus varied considerably among roosts what may be explained by the fact this species can roost alone, in couples, in harems or in groups of bachelor males. Phyllostomus hastatus was observed in cohabitation with three other species: Molossus rufus, Molossus molossus and Myotis nigricans. Due to the frequency of cohabitation observed between P. hastatus and species of the genus Molossus, one or more advantages for the members of this association may be expected. The simultaneous usage of a feeding roost by a group of bachelor males is unknown information in the literature, and may suggest that this kind of group may interact with each other even when away from their diurnal roosts.

  13. Ultrastructure of spermatogenesis in the short-tailed fruit bat, Carollia perspicillata (Chiroptera: Phyllostomidae: Carollinae).

    Science.gov (United States)

    Beguelini, Mateus R; Bueno, Larissa M; Caun, Dianelli L; Taboga, Sebastião R; Morielle-Versute, Eliana

    2014-01-01

    Among species of the Chiroptera, spermatogenesis and the fully differentiated spermatozoa differ in morphological and ultrastructural detail. This study therefore aimed to ultrastructurally characterize the spermatogenesis and the spermatozoa of Carollia perspicillata (Phyllostomidae) and compare the process with other species of bats and mammals. The differentiation of spermatogonia is similar to other bats and to Primates, with three main spermatogonia types: Ad, Ap, and B. Meiotic divisions proceed similarly to those of most mammals and spermiogenesis is clearly divided into 12 steps, in the middle of the range of developmental steps for bats (9-16 steps). The process of acrosome formation is similar to that found in Platyrrhinus lineatus, with the acrosome formed by two different types of proacrosomal vesicles. The ultrastructure of the spermatozoon is similar to other bats already described and resembles the typical mammalian sperm model; however, its morphology differs from other mammals such as marsupials and rodents, on account of a simpler spermatozoon head morphology, which indicates a pattern that is more closely related to the sperm cells of humans and other primates. Our data demonstrated that spermatogenesis in C. perspicillata presents great ultrastructural similarities to P. lineatus. This pattern is not surprising, because both species belong to the same family (Phyllostomidae); however, it is observed that C. perspicillata presents some characteristics that are more closely related to phylogenetically distant species, such as Myotis nigricans (Vespertilionidae), which is a fact that deserves attention.

  14. The Sponge Community of a Subtidal Area with Hydrothermal Vents: Milos Island, Aegean Sea

    Science.gov (United States)

    Pansini, M.; Morri, C.; Bianchi, C. N.

    2000-11-01

    Sponges were sampled by SCUBA diving at six subtidal rocky sites, three of which were close to hydrothermal vents, a common feature on the sea-floor off the south-east coast of Milos. Twenty-five species (2 Calcarea and 23 Demospongiae) were found, few compared with the 589 recorded for the Mediterranean, but an important addition to the scant information on the sponge fauna of the Aegean Sea. The number of species found at vent sites was consistently higher than that found at non-vent sites, but no vent-obligate species could be identified. However, Geodia cydonium and three species of Cliona ( C. copiosa, C. nigricans and C. rhodensis) showed a tendency to colonize vent areas. The former might take advantage of increased silica availability, the latter of the enhanced deposition of carbonates near vents. Substratum cover by sponges (estimated from wire-framed photographs of 0·7 m 2), varied greatly both among and within sites, mostly according to slope. Most sponge species preferred vertical to overhanging, shaded substrata. Proximity to vents seemed to have little or no influence on sponge cover, notwithstanding a primary effect on species diversity.

  15. Demographics of the spawning aggregations of four catostomid species in the Savannah River, South Carolina and Georgia, USA

    Science.gov (United States)

    Grabowski, T.B.; Ratterman, N.L.; Isely, J.J.

    2008-01-01

    Differences in the life history strategies employed by otherwise ecologically similar species of a fish assemblage may be an important factor in the coexistence of these species and is an essential consideration in the conservation and management of these assemblages. We collected scales to determine age and growth of four species of the catostomid assemblage (northern hogsucker Hypentelium nigricans, spotted sucker Minytrema melanops, notchlip redhorse Moxostoma collapsum and robust redhorse Moxostoma robustum) of the Savannah River, Georgia-South Carolina in spring 2004 and 2005. Robust redhorse was the largest species; reaching sexual maturity at an older age and growing faster as a juvenile than the other species. Spotted sucker did not achieve the same size as robust redhorse, but reached sexual maturity at younger ages. Notchlip redhorse was intermediate between the abovementioned two species in age at maturity and size. Northern hogsucker was the smallest species of the assemblage and reached the sexual maturity at the age of three. Both robust redhorse and spotted sucker were sexually dimorphic in size-at-age. The range of life history strategies employed by Savannah River catostomids encompasses the range of life history strategies exhibited within the family as a whole. ?? 2007 Blackwell Munksgaard.

  16. Spatial and temporal segregation of spawning habitat by catostomids in the Savannah River, Georgia and South Carolina, U.S.A.

    Science.gov (United States)

    Grabowski, T.B.; Isely, J.J.

    2007-01-01

    Spawning aggregations of five species of catostomids were observed on the two mid-channel gravel bars of the Savannah River, Georgia and South Carolina, in 2004 and 2005 to assess the degree of spatial and temporal overlap in the use of this habitat and determine the habitat preferences leading to segregation. Spawning catostomids showed a considerable amount of temporal overlap in their use of these mid-channel gravel bars. The observed temporal overlap was consistent between 2004 and 2005 and corresponded to temperatures at which species were present. The distribution of catostomids was not uniform at the upstream gravel bar. Carpsuckers Carpiodes sp., spotted sucker Minytrema melanops and robust redhorse Moxostoma robustum both demonstrated some spatial overlap with notchlip redhorse Moxostoma collapsum; however, their overall distributions were different from one another. Northern hogsucker Hypentelium nigricans was present across the gravel bars, apparently as an egg predator. Spawning catostomids segregated based on flow, depth, slope and substratum size. Whether due to limited habitat availability or changes in the timing of reproduction due to altered cues, temporal and spatial overlap occurs between spawning catostomids despite the apparent partitioning of available spawning habitat. It is unclear, however, if this overlap results in excessive mortality in the early life-history stages of these species. Results suggest spatial overlap among catostomid species was minimized due to species spawning in areas within a narrow range of conditions. Intraspecific interactions such as nest site superimposition or disturbance may be a concern. ?? 2007 The Fisheries Society of the British Isles.

  17. Recombination in the eggs and sperm in a simultaneously hermaphroditic vertebrate.

    Science.gov (United States)

    Theodosiou, L; McMillan, W O; Puebla, O

    2016-12-14

    When there is no recombination (achiasmy) in one sex, it is in the heterogametic one. This observation is so consistent that it constitutes one of the few patterns in biology that may be regarded as a 'rule' and Haldane (Haldane 1922 J. Genet. 12, 101-109. (doi:10.1007/BF02983075)) proposed that it might be driven by selection against recombination in the sex chromosomes. Yet differences in recombination rates between the sexes (heterochiasmy) have also been reported in hermaphroditic species that lack sex chromosomes. In plants-the vast majority of which are hermaphroditic-selection at the haploid stage has been proposed to drive heterochiasmy. Yet few data are available for hermaphroditic animals, and barely any for hermaphroditic vertebrates. Here, we leverage reciprocal crosses between two black hamlets (Hypoplectrus nigricans, Serranidae), simultaneously hermaphroditic reef fishes from the wider Caribbean, to generate high-density egg- and sperm-specific linkage maps for each parent. We find globally higher recombination rates in the eggs, with dramatically pronounced heterochiasmy at the chromosome peripheries. We suggest that this pattern may be due to female meiotic drive, and that this process may be an important source of heterochiasmy in animals. We also identify a large non-recombining region that may play a role in speciation and local adaptation in Hypoplectrus.

  18. Vegetation of eastern Unalaska Island, Aleutian Islands, Alaska

    Science.gov (United States)

    Talbot, Stephen S.; Schofield, Wilfred B.; Talbot, Sandra L.; Daniëls, Fred J. A.

    2010-01-01

    Plant communities of Unalaska Island in the eastern Aleutian Islands of western Alaska, and their relationship to environmental variables, were studied using a combined Braun-Blanquet and multivariate approach. Seventy relevés represented the range of structural and compositional variation in the matrix of vegetation and landform zonation. Eleven major community types were distinguished within six physiognomic–ecological groups: I. Dry coastal meadows: Honckenya peploides beach meadow, Leymus mollis dune meadow. II. Mesic meadows: Athyrium filix-femina – Aconitum maximum meadow, Athyrium filix-femina – Calamagrostis nutkaensis meadow, Erigeron peregrinus – Thelypteris quelpaertensis meadow. III. Wet snowbed meadow: Carex nigricans snowbed meadow. IV. Heath: Linnaea borealis – Empetrum nigrum heath, Phyllodoce aleutica heath, Vaccinium uliginosum – Thamnolia vermicularis fellfield. V. Mire: Carex pluriflora – Plantago macrocarpa mire. VI. Deciduous shrub thicket: Salix barclayi – Athyrium filix-femina thicket. These were interpreted as a complex gradient primarily influenced by soil moisture, elevation, and pH. Phytogeographical and syntaxonomical analysis of the plant communities indicated that the dry coastal meadows, most of the heaths, and the mire vegetation belonged, respectively, to the widespread classes Honckenyo–Elymetea, Loiseleurio–Vaccinietea, and Scheuchzerio–Caricetea, characterized by their circumpolar and widespread species. Amphi-Beringian species were likely diagnostic of amphi-Beringian syntaxa, many of these yet to be described.

  19. Dados ecológicos dos quirópteros da Reserva Volta Velha, Itapoá, Santa Catarina, Brasil Ecological data of Chiroptera from Reserva Volta Velha, Itapoá, Santa Cantarina, Brazil

    Directory of Open Access Journals (Sweden)

    Elenise A. Bastos Sipinski

    1995-09-01

    Full Text Available A study on the ecology of Chiroptera was made at the Reserve of Volta Velha on the Northern coast of Santa Catarina. This study aimed at knowing the frequency of the individuals colleeted in each season, part of their feeding diet and their time of activity as well as their breeding activity. The investigation was carried out from March, 1990 to February, 1991 by means of periodical captures which ocurred during four days each month totalizing 144 hours of net use. One hundred and thirty-five individuals belonging to 15 different species were caught. Sturnira lilium (Geoffroy, 1810, Artibeus lituratus (Olfers, 1818 and Myotis nigricans (Schinz, 1821 were captured in the highest number throughout the seasons of the year. Fruit-eating bats confirm their feeding preference for Cecropiaceae, Solanaceae. Myrtaceae and Piperaceae. The births occurred mostly in seasons other than the dry. Around 1:15 hours after sunset was the time when the highest numher of individuals were collected in the nets.

  20. Quirópteros do Parque Estadual da Pedra Branca, Rio de Janeiro, Brasil (Mammalia, Chiroptera Bats from Pedra Branca State Park, Rio de Janeiro, Brazil (Mammalia, Chiroptera

    Directory of Open Access Journals (Sweden)

    Daniela Dias

    2002-12-01

    Full Text Available A survey of chiropteran fauna was conducted during the period from March 1994 to May 1998 in Pedra Branca State Park, Rio de Janeiro, Rio de Janeiro State. A total of 681 specimens of 24 species were recorded: Chrotopterus auritus (Peters, 1856; Micronycteris megalotis (Gray, 1842; Micronycteris minuta (Gervais, 1856; Mimmon bennettii (Gray, 1838; Phyllostomus hastatus (Pallas, 1767; Tonatia bidens (Von Spix, 1823; Lonchophylla bokermanni Sazima, Vizotto & Taddei, 1978; Lonchophylla mordax Thomas, 1903; Anoura caudifera (E. Geoffroy, 1818; Glosso-phaga soricina (Pallas, 1766; Carollia perspicillata (Linnaeus, 1758; Artibeus fimbriatus Gray, 1838; Artibeus lituratus (Olfers, 1818; Artibeus obscuras Schinz, 1821; Chiroderma doriae Thomas, 1891; Platyrrhinus lineatus (E. Geoffroy, 1810; Platyrrhinus recifinus (Thomas, 1901; Sturnira lilium (E. Geoffroy, 1810; Vampyressa pusilla (Wagner, 1843; Desmodus rotundus (E. Geoffroy, 1810; Diphylla ecaudata Von Spix, 1823; Eptesicus brasiliensis (DesMarest, 1819; Myotis nigricans (Schinz, 1821 and Molossus molossus (Palas, 1766. One external (forearm length and 13 cranial meansurements were studied for 23 species. The meansurements of male and female specimens were treated separately. Comments about some taxonomic respects for some species studied are also included.

  1. Phytochemical investigation and antimicrobial activity of Caesalpinia bonduc (linn Roxb seeds

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    V. Subramani

    2014-06-01

    Full Text Available The aim of the study was to investigate phytochemical properties, antimicrobial activity and trace metal concentrations of Caesalpinia bonducella. The phytochemical screening of the extracts of leaves of C. bonducella revealed the presence of bioactive compounds such as Steroid, Triterpenoids, Reducing Sugar(A, Reducing Sugar(B, Sugars, Flavonoids, Saponin, Amino acids with absence of Alkaloids, Phenolic Compounds, Catachins, Tannins, Anthroquinones.  The ethanol solvent was used for extraction and was used to screen the antimicrobial activity of C. bonducella leaves against certain pathogens by disc diffusion method. In the antimicrobial study, in bacteria, the test sample was most effective against Escherichia coli NCIM 2931 (B2 while smaller effect was noticed from Staphylococcus aureus NCIM 5021 (B3. In fungi, this was effective against Epidermophyton floccosum var. nigricans MTCC 613 (F2 whereas smaller effect was observed in Candida glabrata MTCC 3984 (F1. All the microbial strains depict higher sensitivity to the higher concentration (1.2 mg / disc for the test sample when compared to the positive control except bacterial strains such as Bacillus subtilis NCIM 2920 (B1 and Staphylococcus aureus NCIM 5021 (B3.  The result was supported the view that C. bonducella is a potent antimicrobial agent compared with the conventional antibiotic. The concentrations of trace metals in plants were not cross the standard level. Hence, it is signified that Aloe vera plant extract is safe to be used as an antimicrobial agent.

  2. Enzootic Arbovirus Surveillance in Forest Habitat and Phylogenetic Characterization of Novel Isolates of Gamboa Virus in Panama.

    Science.gov (United States)

    Eastwood, Gillian; Loaiza, Jose R; Pongsiri, Montira J; Sanjur, Oris I; Pecor, James E; Auguste, Albert J; Kramer, Laura D

    2016-04-01

    Landscape changes occurring in Panama, a country whose geographic location and climate have historically supported arbovirus transmission, prompted the hypothesis that arbovirus prevalence increases with degradation of tropical forest habitats. Investigations at four variably degraded sites revealed a diverse array of potential mosquito vectors, several of which are known vectors of arbovirus pathogens. Overall, 675 pools consisting of 25,787 mosquitoes and representing 29 species from nine genera (collected at ground and canopy height across all habitats) were screened for cytopathic viruses on Vero cells. We detected four isolates of Gamboa virus (family:Bunyaviridae; genus:Orthobunyavirus) from pools of Aedeomyia squamipennis captured at canopy level in November 2012. Phylogenetic characterization of complete genome sequences shows the new isolates to be closely related to each other with strong evidence of reassortment among the M segment of Panamanian Gamboa isolates and several other viruses of this group. At the site yielding viruses, Soberanía National Park in central Panama, 18 mosquito species were identified, and the predominant taxa included A. squamipennis,Coquillettidia nigricans, and Mansonia titillans.

  3. Culicidae (Diptera selection of humans, chickens and rabbits in three different environments in the province of Chaco, Argentina

    Directory of Open Access Journals (Sweden)

    Marina Stein

    2013-08-01

    Full Text Available Studies were conducted to determine the selection of humans, chickens and rabbits by Culicidae in three different environments in the province of Chaco, Argentina. Mosquitoes were collected fortnightly using cylindrical metal traps containing animal bait (chickens and rabbits. The mosquitoes were collected between June 2001-May 2002. During the same period and with the same frequency, mosquitoes biting the human operators of the traps were collected during the first 15 min of exposure within different time intervals: from 09:00 am-11:00 am, 01:00 pm-03:00 pm, 05:00 pm-07:00 pm and 09:00 pm-10:00 pm. A total of 19,430 mosquitoes of 49 species belonging to 10 genera were collected. Culex species mainly selected chicken bait and Wyeomyia species selected rabbit bait. Ochlerotatus and Psorophora species were more abundant in rabbit-baited traps. Anopheles triannulatus, Coquillettidia nigricans, Ochlerotatus scapularis, Mansonia titillans and Psorophora albigenu showed a strong attraction for human bait. The Anopheles, Coquillettidia, Culex and Mansonia species were more active between 05:00 pm-09:00 pm, while Ochlerotatus, Psorophora, Haemagogus and Wyeomyia were most active from 09:00 am-07:00 pm. This study provides additional information about the biology and ecology of arbovirus vectors in Chaco.

  4. A NEW SPECIES OF VOLUCELLA GEOFFROY, 1762 FROM CHINA (DIPTERA,SYRPHISAE)%中国蜂蚜蝇属一新种记述(食蚜蝇科,蜂蚜蝇族)

    Institute of Scientific and Technical Information of China (English)

    霍科科; 余治家; 王新辅

    2009-01-01

    记述采自中国宁夏回族自治区六盘山自然保护区蜂蚜蝇属1新种:六盘山蜂蚜蝇Volucdla livpanshanensis sp. nov..新种近似Volucella thompsoni Choi et al.,2006.新种雄性复眼接缝长,约等于额长,腹部第3背板前缘具狭横斑,第4背板后缘向前凹入,使生殖后节背面可见;雌性复眼被毛极稀疏,腹部第3背板前缘具黄白色狭横带.模式标本保存于陕西理工学院动植物标本馆.黑蜂蚜蝇V.nigricans和黄盾蜂蚜蝇V.pellucens tabanoides为宁夏首次记录.文中给出新种描述、特征图和种团分类检索表.%In the paper, a new species of Volucella Geoffroy, 1762 is described from Liupanshan Natural Reserve, Ningxia Hui Autonomous Region: Volucella livpanshanensis sp. nov. The type specimens are held in the Museum of Animal and Botany, Shaanxi University of Technology. During the investigation Volucella nigricans and Volucella pellucens tabanoides are recorded for the first from Ningxia Hni Autonomous Region.

  5. Diversity of mycorrhizal fungi of terrestrial orchids: compatibility webs, brief encounters, lasting relationships and alien invasions.

    Science.gov (United States)

    Bonnardeaux, Yumiko; Brundrett, Mark; Batty, Andrew; Dixon, Kingsley; Koch, John; Sivasithamparam, K

    2007-01-01

    The diversity of mycorrhizal fungi associated with an introduced weed-like South African orchid (Disa bracteata) and a disturbance-intolerant, widespread, native West Australian orchid (Pyrorchis nigricans) were compared by molecular identification of the fungi isolated from single pelotons. Molecular identification revealed both orchids were associated with fungi from diverse groups in the Rhizoctonia complex with worldwide distribution. Symbiotic germination assays confirmed the majority of fungi isolated from pelotons were mycorrhizal and a factorial experiment uncovered complex webs of compatibility between six terrestrial orchids and 12 fungi from Australia and South Africa. Two weed-like (disturbance-tolerant rapidly spreading) orchids - D. bracteata and the indigenous Australian Microtis media, had the broadest webs of mycorrhizal fungi. In contrast, other native orchids had relatively small webs of fungi (Diuris magnifica and Thelymitra crinita), or germinated exclusively with their own fungus (Caladenia falcata and Pterostylis sanguinea). Orchids, such as D. bracteata and M. media, which form relationships with diverse webs of fungi, had apparent specificity that decreased with time, as some fungi had brief encounters with orchids that supported protocorm formation but not subsequent seedling growth. The interactions between orchid mycorrhizal fungi and their hosts are discussed.

  6. Changes in abundance and spatial distribution of geese molting near Teshekpuk Lake, Alaska: Interspecific competition or ecological change?

    Science.gov (United States)

    Flint, P.L.; Mallek, E.J.; King, R.J.; Schmutz, J.A.; Bollinger, K.S.; Derksen, D.V.

    2008-01-01

    Goose populations molting in the Teshekpuk Lake Special Area of the National Petroleum Reserve-Alaska have changed in size and distribution over the past 30 years. Black brant (Branta bernicla nigricans) are relatively stable in numbers but are shifting from large, inland lakes to salt marshes. Concurrently, populations of greater white-fronted geese (Anser albifrons frontalis) have increased seven fold. Populations of Canada geese (Branta canadensis and/or B. hutchinsii) are stable with little indication of distributional shifts. The lesser snow goose (Anser caerulescens caerulescens) population is proportionally small, but increasing rapidly. Coastline erosion of the Beaufort Sea has altered tundra habitats by allowing saltwater intrusion, which has resulted in shifts in composition of forage plant species. We propose two alternative hypotheses for the observed shift in black brant distribution. Ecological change may have altered optimal foraging habitats for molting birds, or alternatively, interspecific competition between black brant and greater white-fronted geese may be excluding black brant from preferred habitats. Regardless of the causative mechanism, the observed shifts in species distributions are an important consideration for future resource planning. ?? 2007 Springer-Verlag.

  7. Ciliates and their picophytoplankton-feeding activity in a high-altitude warm-monomictic saline lake.

    Science.gov (United States)

    Pestová, Dana; Macek, Miroslav; Elena Martínez Pérez, María

    2008-02-01

    The impact of feeding on autotrophic picoplankton (APP) on the ciliate composition of the assemblage was surveyed monthly along a depth gradient in the maar crater, athalassohaline, warm monomictic Lake Alchichica (Puebla, Mexico) from June 2003 to December 2005. Numbers of APP were evaluated from their autofluorescence. DAPI staining and the Fluorescently Labeled Bacteria technique were employed to count ciliates and estimate their feeding rates. A total of 38 taxa of ciliates have been identified using Quantitative Protargol Staining. Peritrichs followed by minute spirotrichs (particularly Halteria grandinella) often numerically dominated the ciliate assemblage and emerged as the most efficient APP feeders. A maximum of 54 ciliate cells ml(-1) was observed in the surface layer at the end of the mixing period, during the development of diatoms (Cyclotella alchichicana), the cyanobacterial bloom (Nodularia sp.) and its decay. Vorticellids (Pelagovorticella natans, Vorticella sp.) had the highest APP uptake (median 130 APP cil(-1) h(-1)). Mixotrophic Euplotes cf. daidaleos were important APP grazers near the oxycline. Scuticociliates (Cyclidium glaucoma, Uronema nigricans and an anaerobic cf. Isocyclidium globossum), were numerically dominant within the hypolimnetic assemblages and did not ingest APP. Generally, APP were not an important food source for the majority of the ciliate assemblage, being positively selected by a few species during the APP decay in aerobic and microaerobic conditions.

  8. Artisanal fisheries of the Xingu River basin in Brazilian Amazon.

    Science.gov (United States)

    Isaac, V J; Almeida, M C; Cruz, R E A; Nunes, L G

    2015-08-01

    The present study characterises the commercial fisheries of the basin of the Xingu River, a major tributary of the Amazon River, between the towns of Gurupá (at the mouth of the Amazon) and São Félix do Xingu. Between April, 2012, and March, 2014, a total of 23,939 fishing trips were recorded, yielding a total production of 1,484 tons of fish, harvested by almost three thousand fishers. The analysis of the catches emphasizes the small-scale and artisanal nature of the region's fisheries, with emphasis on the contribution of the motorised canoes powered by "long-tail" outboard motors. Larger motorboats operate only at the mouth of the Xingu and on the Amazon. Peacock bass (Cichla spp.), croakers (Plagioscion spp.), pacu (a group containing numerous serrasalmid species), aracu (various anostomids), and curimatã (Prochilodus nigricans) together contributed more than 60% of the total catch. Mean catch per unit effort was 18 kg/fisher-1.day-1, which varied among fishing methods (type of vessel and fishing equipment used), river sections, and time of the year. In most cases, yields varied little between years (2012 and 2013). The technical database provided by this study constitutes an important resource for the regulation of the region's fisheries, as well as for the evaluation of future changes resulting from the construction of the Belo Monte dam on the Xingu River.

  9. Diurnal observations on the behavioral ecology of Gymnothorax moringa (Cuvier) and Muraena miliaris (Kaup) on a Caribbean coral reef

    Science.gov (United States)

    Abrams, R. W.; Abrams, M. D.; Schein, M. W.

    1983-09-01

    Activities of muraenids, primarily Gymnothorax moringa and Muraena miliaris, were observed on a Caribbean coral reef with a view to further understanding their role in the reef ecosystem. Other muraenid species included in the total of 198 sightings were Echidna catenata, Enchelycore nigricans, and an unidentified brown moray. The five species were unequally distributed among three basic habitats (sand, coral head, reef rock) available on the reef. Nine particular holes accounted for 52.5% of the total sightings, although hundreds of other seemingly appropriate sites were available. The eels (except M. miliaris) were transient with respect to given holes and particular sections of the reef. While some G. moringa were sighted in the same holes for several consecutive days, M. miliaris individuals remained in the same coral heads throughout the 6-week study period. Muraenids observed in this study showed high tolerances for and were tolerated by other fishes (including other morays) and invertebrates on the reef. They appeared to be opportunistic, roving predators and were not strictly nocturnal. Distinct behavioral interactions and displays between muraenids and reef fish were observed.

  10. Gross mismatch between thermal tolerances and environmental temperatures in a tropical freshwater snail: climate warming and evolutionary implications.

    Science.gov (United States)

    Polgar, Gianluca; Khang, Tsung Fei; Chua, Teddy; Marshall, David J

    2015-01-01

    The relationship between acute thermal tolerance and habitat temperature in ectotherm animals informs about their thermal adaptation and is used to assess thermal safety margins and sensitivity to climate warming. We studied this relationship in an equatorial freshwater snail (Clea nigricans), belonging to a predominantly marine gastropod lineage (Neogastropoda, Buccinidae). We found that tolerance of heating and cooling exceeded average daily maximum and minimum temperatures, by roughly 20°C in each case. Because habitat temperature is generally assumed to be the main selective factor acting on the fundamental thermal niche, the discordance between thermal tolerance and environmental temperature implies trait conservation following 'in situ' environmental change, or following novel colonisation of a thermally less-variable habitat. Whereas heat tolerance could relate to an historical association with the thermally variable and extreme marine intertidal fringe zone, cold tolerance could associate with either an ancestral life at higher latitudes, or represent adaptation to cooler, higher-altitudinal, tropical lotic systems. The broad upper thermal safety margin (difference between heat tolerance and maximum environmental temperature) observed in this snail is grossly incompatible with the very narrow safety margins typically found in most terrestrial tropical ectotherms (insects and lizards), and hence with the emerging prediction that tropical ectotherms, are especially vulnerable to environmental warming. A more comprehensive understanding of climatic vulnerability of animal ectotherms thus requires greater consideration of taxonomic diversity, ecological transition and evolutionary history.

  11. Carryover effects associated with winter location affect fitness, social status, and population dynamics in a long-distance migrant

    Science.gov (United States)

    Sedinger, James S.; Schamber, Jason L.; Ward, David H.; Nicolai, Christopher A.; Conant, Bruce

    2011-01-01

    We used observations of individually marked female black brant geese (Branta bernicla nigricans; brant) at three wintering lagoons on the Pacific coast of Baja California—Laguna San Ignacio (LSI), Laguna Ojo de Liebre (LOL), and Bahía San Quintín (BSQ)—and the Tutakoke River breeding colony in Alaska to assess hypotheses about carryover effects on breeding and distribution of individuals among wintering areas. We estimated transition probabilities from wintering locations to breeding and nonbreeding by using multistratum robust-design capture-mark-recapture models. We also examined the effect of breeding on migration to wintering areas to assess the hypothesis that individuals in family groups occupied higher-quality wintering locations. We used 4,538 unique female brant in our analysis of the relationship between winter location and breeding probability. All competitive models of breeding probability contained additive effects of wintering location and the 1997–1998 El Niño–Southern Oscillation (ENSO) event on probability of breeding. Probability of breeding in non-ENSO years was 0.98 ± 0.02, 0.68 ± 0.04, and 0.91 ± 0.11 for females wintering at BSQ, LOL, and LSI, respectively. After the 1997–1998 ENSO event, breeding probability was between 2% (BSQ) and 38% (LOL) lower than in other years. Individuals that bred had the highest probability of migrating the next fall to the wintering area producing the highest probability of breeding.

  12. Taxonomic review of the Sebastes pachycephalus complex (Scorpaeniformes: Scorpaenidae).

    Science.gov (United States)

    Kai, Yoshiaki; Nakabo, Tetsuji

    2013-01-01

    A taxonomic review of the Sebastes pachycephalus complex established the existence of two valid species, S. pachycephalus and S. nudus. Similarities between them include: cranium armed dorsally with robust preocular, supraocular, postocular, and parietal spines; interorbital space concave; lower jaw lacking scales, shorter than upper jaw; thickened rays in ventral half of pectoral fin; dorsal fin usually with 13 spines and 12 soft-rays; pored lateral line scales 27-35 (usually 29-33). However, S. pachycephalus is distinguishable from the latter in having minute scales below the entire dorsal-fin spine base (vs. lacking minute scales below first to fifth or variously to the posteriormost spine in the latter), dark spots scattered on the dorsal, anal and caudal fins (vs. no distinct dark spots), and lacking distinct colored markings on the dorsum (vs. yellow or reddish-brown markings present). Although both species occur off the southern Korean Peninsula and in the Bohai and Yellow Seas, in Japanese waters, the former is distributed from northern Honshu Is. southward to southern Kyushu Is., whereas the latter extends from southern Hokkaido southward along the Pacific coast of Japan to Kanagawa, and along the Sea of Japan coast to northern Kyushu Is., including the Seto Inland Sea. Sebastes nigricaus, S. nigricans, and S. latus are confirmed as junior synonyms of S. pachycephalus, and S. chalcogrammus as junior synonym of S. nudus, based on the examination of type specimens.

  13. Fishing top predators indirectly affects condition and reproduction in a reef-fish community.

    Science.gov (United States)

    Walsh, S M; Hamilton, S L; Ruttenberg, B I; Donovan, M K; Sandin, S A

    2012-03-01

    To examine the indirect effects of fishing on energy allocation in non-target prey species, condition and reproductive potential were measured for five representative species (two-spot red snapper Lutjanus bohar, arc-eye hawkfish Paracirrhites arcatus, blackbar devil Plectroglyphidodon dickii, bicolour chromis Chromis margaritifer and whitecheek surgeonfish Acanthurus nigricans) from three reef-fish communities with different levels of fishing and predator abundance in the northern Line Islands, central Pacific Ocean. Predator abundance differed by five to seven-fold among islands, and despite no clear differences in prey abundance, differences in prey condition and reproductive potential among islands were found. Body condition (mean body mass adjusted for length) was consistently lower at sites with higher predator abundance for three of the four prey species. Mean liver mass (adjusted for total body mass), an indicator of energy reserves, was also lower at sites with higher predator abundance for three of the prey species and the predator. Trends in reproductive potential were less clear. Mean gonad mass (adjusted for total body mass) was high where predator abundance was high for only one of the three species in which it was measured. Evidence of consistently low prey body condition and energy reserves in a diverse suite of species at reefs with high predator abundance suggests that fishing may indirectly affect non-target prey-fish populations through changes in predation and predation risk.

  14. Pranked by Audubon: Constantine S. Rafinesque's description of John James Audubon's imaginary Kentucky mammals

    Science.gov (United States)

    Woodman, Neal

    2016-01-01

    The North American naturalist Constantine S. Rafinesque spent much of the year 1818 engaged in a solo journey down the Ohio River Valley to explore parts of what was then the western United States. Along the way, he visited a number of fellow naturalists, and he spent more than a week at the Henderson, Kentucky, home of artist and ornithologist John James Audubon. During the succeeding two years, Rafinesque published descriptions of new species that resulted from his expedition, including eleven species of fishes that eventually proved to have been invented by Audubon as a prank on the credulous naturalist. Less well known are a number of “wild rats” described by Rafinesque that include one recognized species (Musculus leucopus) and ten other, imaginary “species” fabricated by Audubon (Gerbillus leonurus, G. megalops, Spalax trivittata, Cricetus fasciatus, Sorex cerulescens, S. melanotis, Musculus nigricans, Lemmus albovittatus, L. talpoides, Sciurus ruber). Rafinesque's unpublished sketches of these animals provide important insight regarding the supposed nature of the animals invented by Audubon and ultimately published by Rafinesque.

  15. Modified method for external attachment of transmitters to birds using two subcutaneous anchors

    Science.gov (United States)

    Lewis, T.L.; Flint, P.L.

    2008-01-01

    Of the transmitter attachment techniques for birds, the subcutaneous anchor provides a secure attachment that yields relatively few secondary effects. However, the use of subcutaneous anchors has been limited by transmitter size and retention time. Using a modified method of attachment that utilized two subcutaneous anchors, we deployed 69 GPS transmitters, plus 13 VHF transmitters that were similar in size and weight to GPS models, on Pacific Black Brant (Branta bernicla nigricans). Prior to our study, only harnesses were used for attaching GPS transmitters on birds, mainly because GPS transmitters are too large for other external attachment techniques and implantation in the body cavity attenuates the GPS signal. Thus, to increase the size capacity of anchor attachment and to avoid the well-documented negative effects of harnesses on behavior and survival, we added a second anchor at the transmitter's posterior end. The double-anchor attachment technique was quickly and easily accomplished in the field, requiring bird handling times of killed by hunters (3-6 mo after deployment) retained their GPS transmitters. For studies involving the use of relatively large transmitters, the double-anchor method appears to provide a viable alternative for external attachment. ?? 2008 Association of Field Ornithologists.

  16. ESTABILIDADE QUÍMICA E MICROBIOLÓGICA DE "MINCED FISH" DE PEIXES AMAZÔNICOS DURANTE O CONGELAMENTO

    Directory of Open Access Journals (Sweden)

    JESUS Rogério Souza de

    2001-01-01

    Full Text Available Foi estudada a estabilidade química e microbiológica de "minced fish" produzidos, em condições industriais, com espécies de peixes da Amazônia: aracú-comum (Schizodon fasciatus, branquinha-comum (Potamorhina latior, branquinha-de-cabeça-lisa (P. altamazonica, curimatã (Prochilodus nigricans, jaraqui-de-escama-fina (Semaprochilodus taeniurus, jaraqui-de-escama-grossa (S. insignis, mapará (Hypophthalmus edentatus, pacú-comum (Metynnis hypsauchen, pacú-manteiga (Mylossoma duriventre e pirapitinga (Piaractus brachypomum, durante 150 dias sob congelamento a -18±1°C e -36±1°C. Com base no pH, nitrogênio das bases voláteis totais (N-BVT, substâncias reagentes ao ácido tiobarbitúrico (TBARS e contagens de aeróbios mesófilos a 35°C e psicrotróficos a 7°C, e de coliformes totais e fecais (NMP, os "minced fish" obtidos a partir das espécies de peixes estudadas e de misturas de espécies (aracú+curimatã+pirapitinga; jaraqui+branquinha mantiveram-se em condições de consumo, durante o período experimental. Os resultados obtidos são altamente promissores sob o ponto de vista tecnológico.

  17. Biotransformation of sclareolide by filamentous fungi: cytotoxic evaluations of the derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Cano, Arturo [Universidad Nacional Autonoma de Mexico, D.F. (Mexico). Facultad de Estudios Superiores Zaragoza; Ramirez-Apan, Maria Teresa; Delgado, Guillermo, E-mail: delgado@unam.m [Universidad Nacional Autonoma de Mexico, D.F. (Mexico)

    2011-07-01

    Sclareolide (1) was incubated with eight different species of filamentous fungi conventionally used for bio-oxidations. Compound 1 was metabolized with Aspergillus niger in medium A to yield 3-ketosclareolide (2) and 3b-hydroxysclareolide (4), while in medium B (containing major number of nutrients with respect to medium A), compounds 2, 4, 3{alpha},6{beta}-dihydroxysclareolide (16), 1-ketosclareolide (17), 3-keto-15-hydroxysclareolide (18) and 3{beta},15-dihydroxysclareolide (19) were obtained. The biotransformation products 16-19 were found to be new substances. Fermentation of 1 with Cunninghamella blackesleeana using medium A afforded 2 and 4, while using medium B yielded 2, 4, 16 and 17. Compounds 2, 4 and 17 were also obtained with Curvularia lunata. Biotransformation of 1 with Beauveria bassiana yielded 4 in satisfactory yield, with Rhizopus oligosporus and Mucor miehei afforded 2 and 4, while with R. nigricans and Fusarium moliniforme yielded 2, 4 and 16. Cytotoxic evaluation of 1 and the obtained products against selected human cancer cell lines (U251, PC-3, K562, HCT-15, MCF-7 and SKUL-1) indicated that 16 (3{alpha},6{beta}-dihydroxysclareolide) displayed moderate cytotoxic (IC{sub 50} < 100 {mu}M) against U251, PC-3, HCT-15 and MCF-7. (author)

  18. Shaping of the fungal communities isolated from yellow lupin seeds (Lupinus luteus L. throughout storage time

    Directory of Open Access Journals (Sweden)

    Bożena Cwalina-Ambroziak

    2012-12-01

    Full Text Available The object of the experiment were seeds of two traditional cultivars of yellow lupin (Juno and Amulet cultivated in 1999 in two crop-rotation with 20% and 33% yellow lupine contribution. The quantitative and qualitative composition of the fungal community colonizing the seeds were determined in the laboratory conditions after 0.5-, 1.5- and 2.5-year of storage time. In total 1077 fungal colonies were isolated from the lupin seeds. Fungi representing the species of Penicillium - 29.3%, Alternaria alternata - 26.7% and Rhizopus nigricans - 12.7% were isolated most widely. Among the fungi pathogenic to lupin, the species of Colletotrichum gloeosporioides (16.3% isolates was dominant. The crop rotation with 20% lupin reduced the number of fungal colonies colonizing the seeds including the pathogens from the species of C. gloeosporioides. Seed disinfection decreased the total number of fungal colonies isolated from both cultivars. Higher number of C. gloeosporioides isolates was found in the combination with disinfected seeds. More fungal colonies were obtained from seeds of cv. Amulet than from those of cv. Juno. The storage duration had an effect on the population and the composition of species of fungi isolated from seeds of yellow lupine. With longer storage population of Penicillium spp. and Rhizopus spp. increased, whereas the population of C. gloeosporioides decreased.

  19. Interactions among cooling, fungicide and postharvest ripening temperature on peaches

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez-Trujillo, J. Pablo; Cano, Antonio; Artes, Francisco [Postharvest and refrigeration Lab., Dept. of Food Science and Technology, CEBAS-CSIC, Murcia (Spain)

    2000-07-01

    Peach fruit (Prunus persica L. cv. 'Miraflores') harvested at the firm-ripe stage, treated or not with 2 g l{sup -1} iprodione, were cooled or not at 1{sup o}C and ripened at 15 or 20{sup o}C and 95% RH for 10 days. During ripening, weight loss, fungal development and changes in quality parameters (firmness, soluble solids content, titratable acidity, pH and ground and flesh color), and carbon dioxide and ethylene production were monitored. Cooling alone or combined with iprodione avoided Rhizopus nigricans decay during ripening at either ripening temperatures. A skin damage not previously reported on fungicide treated peach was observed at 20{sup o}C. Cooled fruit ripened at 15{sup o}C showed an anomalous respiration rate and ethylene production after the climacteric peak, a loss of firmness and a drop in titratable acidity after 7 days of storage, and reduced endo-polygalacturonase activity in presence of continuous pectinmethylesterase activity during the first week. Cooling before ripening at 20{sup o}C led to the best flavor without excessive total losses. These results helped in the optimization of warming cycles during cold storage used to avoid chilling injuries development on peaches. (Author)

  20. Integrating conjugative elements as vectors of antibiotic, mercury, and quaternary ammonium compound resistance in marine aquaculture environments.

    Science.gov (United States)

    Rodríguez-Blanco, Arturo; Lemos, Manuel L; Osorio, Carlos R

    2012-05-01

    The presence of SXT/R391-related integrating conjugative elements (ICEs) in bacterial strains isolated from fish obtained from marine aquaculture environments in 2001 to 2010 in the northwestern Iberian Peninsula was studied. ICEs were detected in 12 strains taxonomically related to Vibrio scophthalmi (3 strains), Vibrio splendidus (5 strains), Vibrio alginolyticus (1 strain), Shewanella haliotis (1 strain), and Enterovibrio nigricans (2 strains), broadening the known host range able to harbor SXT/R391-like ICEs. Variable DNA regions, which confer element-specific properties to ICEs of this family, were characterized. One of the ICEs encoded antibiotic resistance functions in variable region III, consisting of a tetracycline resistance locus. Interestingly, hot spot 4 included genes providing resistance to rifampin (ICEVspPor2 and ICEValPor1) and quaternary ammonium compounds (QACs) (ICEEniSpa1), and variable region IV included a mercury resistance operon (ICEVspSpa1 and ICEEniSpa1). The S exclusion group was more represented than the R exclusion group, accounting for two-thirds of the total ICEs. Mating experiments allowed ICE mobilization to Escherichia coli strains, showing the corresponding transconjugants' rifampin, mercury, and QAC resistance. These results show the first evidence of ICEs providing rifampin and QAC resistances, suggesting that these mobile genetic elements contribute to the dissemination of antimicrobial, heavy metal, and QAC resistance determinants in aquaculture environments.

  1. Bat flight with bad wings: is flight metabolism affected by damaged wings?

    Science.gov (United States)

    Voigt, Christian C

    2013-04-15

    Infection of North American bats with the keratin-digesting fungus Geomyces destructans often results in holes and ruptures of wing membranes, yet it is unknown whether flight performance and metabolism of bats are altered by such injuries. I conducted flight experiments in a circular flight arena with Myotis albescens and M. nigricans individuals with an intact or ruptured trailing edge of one of the plagiopatagial membranes. In both species, individuals with damaged wings were lighter, had a higher aspect ratio (squared wing span divided by wing area) and an increased wing loading (weight divided by wing area) than conspecifics with intact wings. Bats with an asymmetric reduction of the wing area flew at similar speeds to conspecifics with intact wings but performed fewer flight manoeuvres. Individuals with damaged wings showed lower metabolic rates during flight than conspecifics with intact wings, even when controlling for body mass differences; the difference in mass-specific metabolic rate may be attributable to the lower number of flight manoeuvres (U-turns) by bats with damaged wings compared with conspecifics with intact wings. Possibly, bats compensated for an asymmetric reduction in wing area by lowering their body mass and avoiding flight manoeuvres. In conclusion, it may be that bats suffer from moderate wing damage not directly, by experiencing increased metabolic rate, but indirectly, by a reduced manoeuvrability and foraging success. This could impede a bat's ability to gain sufficient body mass before hibernation.

  2. Zinc deficiency (hypozincemia in local Iraqi cattle

    Directory of Open Access Journals (Sweden)

    Kamal M. Alsaad,

    2011-07-01

    Full Text Available Clinical, hematological, pathological and some biochemical parameters have been studied in local cattle and calves affected naturally with hypozincemia in Mosul, Iraq. The study was conducted on 78 local Iraqi cattle and calves, among these animals, 30 calves were less than six months of age and 38 animals were more than three years old. Ten clinical healthy cattle of different ages were used as control. Affected cattle showed signs of alopecia in different body regions (73.6%, abnormal skin (rough, thickened, wrinkled, cracked and with dandruff (73.6%, paleness of mucous membranes (47.3%, intermittent diarrhoea (39.4%, decreased milk production (31.5% and loss of appetite (26.3%, whereas affected calves showed alopecia in various body regions (90%, abnormal skin (83.3%, decreased growth rate (53.3%, swelling of joints and stiff gait (43.3% and pica (36.6%. No significant difference has been detected in body temperature, whereas respiratory and heart rates were significantly increased in affected animals in comparison with control. Statistical analysis showed significant decrease in the total erythrocytes (TRBCs, hemoglobin (HB and packed cell volume (PCV in diseased cattle and calves and macrocytic normochromic type of anemia was found. The results also indicated significant decrease in lymphocytes and platelets counts, however significant increase was encountered in platelets volume, platelets distribution width, prothrombine time and activated partial thromboplastine time in diseased animals. The biochemical results revealed significant decrease in serum zinc and fibrinogen and haptoglobin level was higher in diseased cattle and calves. Microscopic lesions of the skin of zinc deficient cattle and calves were in the form of epidermal hyperplasia, parakeratosis, hyperkeratosis, acanthosis and the formation of thickened adherent scale.

  3. Immunohistochemical expression of GLUT-1 and Ki-67 in chronic plaque psoriasis.

    Science.gov (United States)

    Abdou, Asmaa G; Maraee, Alaa H; Eltahmoudy, Mohamed; El-Aziz, Reem A

    2013-10-01

    Many inflammatory mediators and other biological markers are upregulated in psoriatic lesions; some of these alterations also persist in nonlesional skin. Glucose is the major source of energy for cells, and glucose transporter 1 is the most common glucose transporter in humans (GLUT-1). The present study aimed at evaluating the pattern of expression of GLUT-1 and Ki-67 in psoriatic skin (involved and uninvolved) and correlating their expression with the clinicopathological parameters in the studied patients. This study was carried out on 30 patients presented with chronic plaque psoriasis and 10 apparently healthy volunteers as a control group. GLUT-1 was not expressed in epidermis of normal skin, whereas it was expressed in 76.6% of uninvolved and 86.7% of involved skin of psoriatic patients, where both the latter differed significantly regarding the intensity (P = 0.001) and localization (P = 0.001) of GLUT-1 expression. The percentage of Ki-67 expression did not differ significantly between involved and uninvolved skin of psoriatic patients, but they were higher than that of normal skin of control group. Nucleolar pattern of Ki-67 expression was significantly associated with male sex (P = 0.05), marked parakeratosis (P = 0.01), and marked angiogenesis (P = 0.05). GLUT-1 expression was associated with degree of acanthosis and percentage of Ki-67 expression. From this study, GLUT-1 is upregulated in psoriatic epidermis and may be involved in facilitation of keratinocyte proliferation in psoriasis. Nucleolar pattern of Ki-67 is an indicator of progressive keratinocyte proliferation in psoriasis.

  4. Focal epithelial hyperplasia in a human immuno-deficiency virus patient treated with laser surgery.

    Science.gov (United States)

    Galanakis, Alexandros; Palaia, Gaspare; Tenore, Gianluca; Vecchio, Alessandro Del; Romeo, Umberto

    2014-07-16

    Focal epithelial hyperplasia (FEH), or Heck's disease, is a rare disease of the oral mucosa; it is mostly found in children or young adults who are immunosuppressed and who live in regions with low socioeconomic status. It is characterized by asymptomatic papules on the oral mucosa, gingiva, tongue, and lips. Healing can be spontaneous, and treatment is indicated if there are aesthetic or functional complications. Human papillomavirus, especially genotypes 13 and 32, has been associated with FEH and is detected in the majority of lesions. Histopathologically, FEH is characterized by parakeratosis, epithelial hyperplasia, focal acanthosis, and fusion and horizontal outgrowth of epithelial ridges. A 37-year-old male patient was referred to the Department of Oral and Maxillofacial Sciences at the Sapienza University of Rome, complaining of numerous exophytic lesions in his mouth. He stated that the lesions were not painful but he had experienced occasional bleeding after incidental masticatory trauma. He had received no previous treatment for the oral lesions. His medical history revealed that he was human immuno-deficiency virus positive and was a smoker with numerous, asymptomatic oral papules clinically and histologically corresponding to FEH. The labial and buccal mucosa were especially affected by lesions. Surgical treatment was performed using a 532-nm potassium titanyl phosphate laser (SmartLite, Deka, Florence, Italy) in continuous mode with a 300 μm fiber and power of 1.4 W (power density 1980.22 W/cm(2)). After anesthesia without vasoconstrictors, the lesions were tractioned with sutures or an Allis clamp and then completely excised. The lesions were preserved in 10% formalin for histological examination, which confirmed the clinical diagnosis of FEH. In this case, the laser allowed excellent control of bleeding, without postoperative sutures, and optimal wound healing.

  5. Significance of clinicopathological correlation in psoriasis

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    Gopal Ambadasrao Pandit

    2015-01-01

    Full Text Available Context: Psoriasis affects about 1.5% to 3% of world′s population. Other papulosquamous dermatoses are Pityriasis rosea, Lichen planus, Seborrheic dermatitis, Pityriasis rubra pilaris and Parapsoriasis. Drug eruptions, tinea corporis, and secondary syphilis may also have papulosquamous morphology. Because all papulosquamous disorders are characterized by scaling papules, clinical confusion may result during their diagnosis. Separation of each of these becomes important because the treatment and prognosis for each tends to be disease-specific. Aim: To study the pattern of clinical and histopathological features of psoriasis of the skin with clinicopathological correlation. Material and methods: The present study of 42 cases of psoriasis of the skin was carried out in the Department of Pathology of a tertiary care centre from December 2009 to October 2011. In this study, the patients which were clinically diagnosed as psoriasis of skin, before starting the treatment and attending the outdoor skin department were selected. Histopathological findings were interpreted in light of clinical details. Results: Out of 42 cases of psoriasis 24 (57.14% were males, 18 (42.86% were females with male to female ratio of 1.33:1. Mean age was 34.45 years. Maximum number of cases 22 (52.38% were encountered in 3rd and 4th decade of life. Histopathological findings: parakeratosis, acanthosis, suprapapillary thinning, Munro microabscesses and hypogranulosis were noted in most of the cases. Conclusion: Histopathology serves as a diagnostic tool and rules out other lesions which mimic psoriasis. The most accurate diagnosis is the one that most closely correlates with clinical outcome and helps to direct the most appropriate clinical intervention.

  6. Atopic dermatitis-like disease and associated lethal myeloproliferative disorder arise from loss of Notch signaling in the murine skin.

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    Alexis Dumortier

    Full Text Available BACKGROUND: The Notch pathway is essential for proper epidermal differentiation during embryonic skin development. Moreover, skin specific loss of Notch signaling in the embryo results in skin barrier defects accompanied by a B-lymphoproliferative disease. However, much less is known about the consequences of loss of Notch signaling after birth. METHODOLOGY AND PRINCIPAL FINDINGS: To study the function of Notch signaling in the skin of adult mice, we made use of a series of conditional gene targeted mice that allow inactivation of several components of the Notch signaling pathway specifically in the skin. We demonstrate that skin-specific inactivation of Notch1 and Notch2 simultaneously, or RBP-J, induces the development of a severe form of atopic dermatitis (AD, characterized by acanthosis, spongiosis and hyperkeratosis, as well as a massive dermal infiltration of eosinophils and mast cells. Likewise, patients suffering from AD, but not psoriasis or lichen planus, have a marked reduction of Notch receptor expression in the skin. Loss of Notch in keratinocytes induces the production of thymic stromal lymphopoietin (TSLP, a cytokine deeply implicated in the pathogenesis of AD. The AD-like associated inflammation is accompanied by a myeloproliferative disorder (MPD characterized by an increase in immature myeloid populations in the bone marrow and spleen. Transplantation studies revealed that the MPD is cell non-autonomous and caused by dramatic microenvironmental alterations. Genetic studies demontrated that G-CSF mediates the MPD as well as changes in the bone marrow microenvironment leading to osteopenia. SIGNIFICANCE: Our data demonstrate a critical role for Notch in repressing TSLP production in keratinocytes, thereby maintaining integrity of the skin and the hematopoietic system.

  7. Chronic graft-versus-host disease in the rat radiation chimera: I. clinical features, hematology, histology, and immunopathology in long-term chimeras

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    Beschorner, W.E.; Tutschka, P.J.; Santos, G.W.

    1982-04-01

    The clinical features, pathology, and immunopathology of chronic graft-versus-host disease (GVHD) developing in the long-term rat radiation chimera are described. At 6 to 12 months post-transplant, the previously stable ACI/LEW chimeras developed patchy to diffuse severe hair loss and thickened skin folds, and had microscopic features resembling scleroderma, Sjogren's syndrome, and chronic hepatitis. Skin histology showed dermal inflammation and acanthosis with atrophy of the appendages, with progression to dermal sclerosis. The liver revealed chronic hepatitis with bile duct injury and proliferation and periportal piecemeal necrosis. The tongue had considerable submucosal inflammation, muscular necrosis, and atrophy and arteritis. The serous salivary glands, lacrimal glands, and bronchi had lymphocytic inflammation and injury to duct, acinar, and mucosal columnar epithelium. The thymus had lymphocyte depletion of the medulla with prominent epithelium. The spleen and lymph nodes had poorly developed germinal centers but increased numbers of plasma cells. IgM was observed along the basement membrane and around the basal cells of the skin and tongue and along the basement membrane of the bile ducts. IgM was present also in the arteries of the tongue. Immunoglobulins eluted from the skin, cross-reacted with the bile duct epithelium and usually with both ACI and Lewis skin. Increased titers of speckled antinuclear antibodies were present in the serum of rats with chronic (GVHD). Chronic GVHD in the long-term rat radiation chimera is very similar to human chronic GVHD and is a potentially excellent model for autoimmune disorders including scleroderma, Sjorgren's syndrome, and chronic hepatitis.

  8. Causative Role of Ureaplasma Urealyticum and other Sexually Transmitted Infections in the Urethral Meatus Polyp Development in Women

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    Tatyana S. Taranina

    2013-09-01

    Full Text Available The objective of this study was the investigation of the influence of ureaplasmal infection on the development of urethral meatus polyps in women. The article presents the results of the examination of women with chronic cystitis and urethritis over a 0.5- to 5-year duration, complicated by the presence of urethral meatus polyps and associated with concomitant Ureaplasma urealyticum and other sexually transmitted infections (STI. This was based on the culture analysis of the cervical and urethral content, and PCR-diagnostics of STI, as well as a complex pathomorphologic study of the resected polyps, including electron microscopy. In this study, 98 women between 45 and 60 years (52.5±4.9 years were examined, who had undergone radiowave resection of the polyps: 52 women were infected by STI, including Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Chlamydia trachomatis and Trichomonas vaginalis, while the remaining 46 women had been diagnosed as not having STI. According to the culture results in the women with STI, U. urealyticum was identified as a monoinfection in 69% of cases, while in the remaining 31% of cases it was evident in the form of mixed infections, mainly in association with Mycoplasma hominis (17.5% and Trichomonas vaginalis (13.5%. Pathomorphological examination of the urethral meatus polyps of the women with U. urealyticum and other STI demonstrated the proliferative character of the remodeling of the surface epithelium with hyperplasia, acanthosis, and keratinization of the stratified squamous epithelium and synchronous changes in the underlying connective tissue - impaired microcirculation and the diffuse inflammatory cell infiltrates with transepithelial leukopedesis. Using electron microscopy in the fibroblasts and plasma cells of the resected polyps the markers of U. urealyticum were detected in patients with negative results of the bacteriological diagnostic methods.

  9. Expression of NF-κB and IL-6 in oral precancerous and cancerous lesions: An immunohistochemical study

    Science.gov (United States)

    Nikitakis, Nikolaos; Sfakianou, Aikaterini; Avgoustidis, Dimitrios; Sklavounou-Andrikopoulou, Alexandra

    2016-01-01

    Background The purpose of this study was to evaluate the immunohistochemical expression of NF-κB and IL-6 in oral premalignant and malignant lesions and to investigate their possible correlation with the presence of subepithelial inflammation. Material and Methods Thirty two oral premalignant lesions, clinically compatible with leukoplakia or erythroplakia, were investigated. Microscopically, 11 of them showed hyperkeratosis and acanthosis (epithelial hyperplasia) and 21 showed dysplasia of varying degrees. Nine cases of OSCC and four control cases of normal oral mucosa were also included in the study. Immunohistochemical staining with NF-κB (p65) and IL-6 was performed. IL-6 and nuclear NF-κB staining were assessed as positive or negative. For cytoplasmic localization of NF-κB, a total score combining intensity and percentage of positive epithelial cells was additionally calculated. The presence of inflammation was also recorded. Results Intensity and total scores for NF-κΒ cytoplasmic immunostaining showed a statistically significant gradual increase from normal mucosa to OSCC (p=0.012 and p=0.026 respectively). Non-statistically significant increased NF-κΒ nuclear localization was detected in dysplasias and OSCCs. Positive statistical correlation was detected between the presence of inflammation and IL-6 expression (p=0.015). No correlation between NF-κΒ and IL-6 was detected. Conclusions NF-κΒ is activated in the early stages of oral carcinogenesis. IL-6 may have an NF-κΒ-independent role, possibly through regulation of the inflammatory response. Key words:NF-κB, IL-6, immunohistochemistry, oral squamous cell carcinoma, oral precancerous lesion. PMID:26595830

  10. Th17 and regulatory T cells contribute to the in situ immune response in skin lesions of Jorge Lobo's disease.

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    Kanashiro-Galo, Luciane; Pagliari, Carla; Barboza, Tania Cristina; de Brito, Arival Cardoso; Xavier, Marilia Brasil; de Oliveira, Clivia Maria Moraes; Unger, Deborah Aben Athar; Sotto, Mirian Nacagami; Quaresma, Juarez Antonio Simões; Duarte, Maria Irma Seixas

    2016-01-01

    Jorge Lobo's disease (JLD) is a chronic granulomatous mycosis described in various Latin American countries. The main objective of the present study was to investigate the possible role of Th17 and Foxp3+ Treg cells in the pathogenesis of Jorge Lobo's disease. Human skin biopsies were submitted to an immunohistochemistry protocol to detect Foxp3, interleukin (IL)-1beta, CD25, IL-6, IL-17, and IL-23. The epidermis presented acanthosis, hyperkeratosis, and frequent presence of fungi. The dermis presented inflammatory infiltrate comprising macrophages, lymphocytes, epithelioid and multinucleated cells, and an intense number of fungi. Foxp3+ Treg cells and IL-17+ cells were visualized in lymphocytes in the inflammatory infiltrate. IL-1, IL-2R (CD25), IL-6, and IL-23 were visualized in the dermis, intermingled with fungal cells, permeating or participating of the granuloma. Following IL-17, the most prominent cytokine was IL-6. IL-23 and cells expressing CD25 were present in fewer number. The comparative analysis between IL-17 and Foxp3 demonstrated a statistically significant increased number of IL-17+ cells. Th17 cells play a role in the immune response of JLD. IL-1beta and IL-6 added to the previously described increased number of TGF-beta would stimulate such pattern of response. Th17 cells could be present as an effort to modulate the local immune response; however, high levels of a Th17 profile could overcome the role of Treg cells. The unbalance between Treg/Th17 cells seems to corroborate with the less effective immune response against the fungus.

  11. Loss of normal profilaggrin and filaggrin in flaky tail (ft/ft) mice: an animal model for the filaggrin-deficient skin disease ichthyosis vulgaris.

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    Presland, R B; Boggess, D; Lewis, S P; Hull, C; Fleckman, P; Sundberg, J P

    2000-12-01

    Flaky tail (gene symbol ft) is an autosomal recessive mutation in mice that results in a dry, flaky skin, and annular tail and paw constrictions in the neonatal period. Previous studies demonstrated that the ft mutation maps to the central region of mouse chromosome 3, in the vicinity of the epidermal differentiation complex, a gene locus that includes many nonkeratin genes expressed in epidermis. In this study we report a detailed characterization of the flaky tail mouse. Affected homozygous ft/ft mice exhibit large, disorganized scales on tail and paw skin, marked attenuation of the epidermal granular layer, mild acanthosis, and orthokeratotic hyperkeratosis. Biochemical analysis demonstrated that ft/ft mice lacked normal high molecular profilaggrin (approximately 500 kDa), and instead expressed a lower molecular weight form of profilaggrin (220 kDa) that is not proteolytically processed to profilaggrin intermediates or filaggrin. Mutant mice lacked the large, irregular F-type keratohyalin granules that contain profilaggrin, and filaggrin was absent from the cornified layers of ft/ft epidermis. The expression of epidermal keratins was unchanged, whereas the cornified envelope proteins involucrin and loricrin were increased in ft/ft epidermis. Cultured ft/ft keratinocytes also synthesized reduced amounts of profilaggrin mRNA and protein, demonstrating that the defect in profilaggrin expression is intrinsic to epidermal cells. These findings demonstrate that flaky tail mice express an abnormal profilaggrin polypeptide that does not form normal keratohyalin F-granules and is not proteolytically processed to filaggrin. We propose that the absence of filaggrin, and in particular the hygroscopic, filaggrin-derived amino acids that are thought to function in epidermal hydration, underlies the dry, scaly skin characteristic of ft/ft mice. This animal model provides a tool for understanding the role of filaggrin in normal epidermal function and may provide insight into

  12. A case of follicular mucinosis in children%儿童毛囊黏蛋白病

    Institute of Scientific and Technical Information of China (English)

    王冲; 梁建华; 刘桂英; 许俊龙; 贾爱华

    2011-01-01

    报告1例儿童毛囊黏蛋白病.患儿男,14岁.因眉间皮肤起浸润性红斑伴眉毛部分脱落半年余就诊.皮肤科检查:眉间偏左侧一约2.5 cm × 3.0 cm浸润性红斑,其上覆少许片状鳞屑,皮损累及处眉毛缺失.皮损组织病理检查:表皮轻度角化不全,棘层增生,真皮毛囊上皮水肿、破坏,少许淋巴细胞移入,胶原间血管壁增厚,周围淋巴细胞及嗜酸性粒细胞浸润;阿新蓝染色示毛囊上皮内较多黏液样物质沉积.诊断:毛囊黏蛋白病.给予复方倍他米松注射液局部封闭治疗后,皮损消退.%A case of follicular mucinosis is reported.A 14-year-old boy presented with half a year history of facial pink infiltrated plaque associated with loss of hair.Histopathologic examination showed parakeratosis.acanthosis in the epidermis, there was a mild lymphocytic infiltration in the dermis around and within the affected follicles.Inflammatory lymphocytic infiltrate , admixed with several eosinophils, located around the vessels.Alcian blue stain was positive within the follicular structures.A diagnosis of follicular mucinosis was finally made.He was successfully treated with intralesional compound betamethasone injection.

  13. Polyomavirus-associated Trichodysplasia spinulosa involves hyperproliferation, pRB phosphorylation and upregulation of p16 and p21.

    Science.gov (United States)

    Kazem, Siamaque; van der Meijden, Els; Wang, Richard C; Rosenberg, Arlene S; Pope, Elena; Benoit, Taylor; Fleckman, Philip; Feltkamp, Mariet C W

    2014-01-01

    Trichodysplasia spinulosa (TS) is a proliferative skin disease observed in severely immunocompromized patients. It is characterized by papule and trichohyalin-rich spicule formation, epidermal acanthosis and distention of dysmorphic hair follicles overpopulated by inner root sheath cells (IRS). TS probably results from active infection with the TS-associated polyomavirus (TSPyV), as indicated by high viral-load, virus protein expression and particle formation. The underlying pathogenic mechanism imposed by TSPyV infection has not been solved yet. By analogy with other polyomaviruses, such as the Merkel cell polyomavirus associated with Merkel cell carcinoma, we hypothesized that TSPyV T-antigen promotes proliferation of infected IRS cells. Therefore, we analyzed TS biopsy sections for markers of cell proliferation (Ki-67) and cell cycle regulation (p16ink4a, p21waf, pRB, phosphorylated pRB), and the putatively transforming TSPyV early large tumor (LT) antigen. Intense Ki-67 staining was detected especially in the margins of TS hair follicles, which colocalized with TSPyV LT-antigen detection. In this area, staining was also noted for pRB and particularly phosphorylated pRB, as well as p16ink4a and p21waf. Healthy control hair follicles did not or hardly stained for these markers. Trichohyalin was particularly detected in the center of TS follicles that stained negative for Ki-67 and TSPyV LT-antigen. In summary, we provide evidence for clustering of TSPyV LT-antigen-expressing and proliferating cells in the follicle margins that overproduce negative cell cycle regulatory proteins. These data are compatible with a scenario of TSPyV T-antigen-mediated cell cycle progression, potentially creating a pool of proliferating cells that enable viral DNA replication and drive papule and spicule formation.

  14. Cutaneous injury-related structural changes and their progression following topical nitrogen mustard exposure in hairless and haired mice.

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    Neera Tewari-Singh

    Full Text Available To identify effective therapies against sulfur mustard (SM-induced skin injuries, various animals have been used to assess the cutaneous pathology and related histopathological changes of SM injuries. However, these efforts to establish relevant skin injury endpoints for efficacy studies have been limited mainly due to the restricted assess of SM. Therefore, we employed the SM analog nitrogen mustard (NM, a primary vesicating and bifunctional alkylating agent, to establish relevant endpoints for efficient efficacy studies. Our published studies show that NM (3.2 mg exposure for 12-120 h in both the hairless SKH-1 and haired C57BL/6 mice caused clinical sequelae of toxicity similar to SM exposure in humans. The NM-induced cutaneous pathology-related structural changes were further analyzed in this study and quantified morphometrically (as percent length or area of epidermis or dermis of skin sections in mice showing these lesions. H&E stained skin sections of both hairless and haired mice showed that NM (12-120 h exposure caused epidermal histopathological effects such as increased epidermal thickness, epidermal-dermal separation, necrotic/dead epidermis, epidermal denuding, scab formation, parakeratosis (24-120 h, hyperkeratosis (12-120 h, and acanthosis with hyperplasia (72-120 h. Similar NM exposure in both mice caused dermal changes including necrosis, edema, increase in inflammatory cells, and red blood cell extravasation. These NM-induced cutaneous histopathological features are comparable to the reported lesions from SM exposure in humans and animal models. This study advocates the usefulness of these histopathological parameters observed due to NM exposure in screening and optimization of rescue therapies against NM and SM skin injuries.

  15. Bloch-Siemens syndrome:a case report and literature review%色素失禁症一例并文献复习

    Institute of Scientific and Technical Information of China (English)

    王彦; 王丽华; 曲燕; 孟现广; 杨秀莉

    2016-01-01

    We herein report a two-month old girl. She had rashes and blisters on trunk and limbs at birth. Dermatological examination revealed reticulate pigmented spots scattered on trunk and extremities. Some dark red papules and blisters in different size were located on both interfemus and wrists. The bilsters had thick walls and the Nikolsky's sign was negative. Most of the lesions were distributed in a splash manner. His-topathologically, the hyperkeratesis, acanthosis and epidermal vesicles filled with eosinophils were found in epidermis, and a small number of lymphocytes and eosinophils infiltration in the superficial dermis capillary were reported. Based on the clinical and histological findings, the final diagnosis was neonatal Bloch-Sie-mens syndrome.%患儿,女,2个月。因躯干及四肢皮疹2个月就诊。皮肤科检查:躯干及四肢皮肤弥漫分布网状色素沉着斑。双股内侧、手腕部见暗红色大小不一丘疹、水疱,水疱壁厚,尼氏征阴性,部分水疱结痂。皮疹呈泼溅状分布。组织病理示:表皮角化过度,棘层增厚,表皮内见多房性水疱,疱液中可见大量嗜酸性粒细胞。真皮浅层毛细血管周围可见少量淋巴细胞及嗜酸性粒细胞浸润。诊断:新生儿色素失禁症。

  16. Role of human papilloma virus in oral leukoplakia

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    Anuja Bhargava

    2016-01-01

    Full Text Available Background: Controversy surrounds regarding the role of human papillomavirus (HPV in oral precancerous and cancerous lesions in India where smokeless, tobacco consumption is rampant. Aims: The present study was carried out with an aim to investigate the presence and type of HPV infection in oropharyngeal leukoplakia and to determine the association of HPV positivity with various patient and lesion characteristics. Settings and Design: Prospective case series. Materials and Methods: A total of fifty confirmed cases of oral leukoplakia (OL, aged 18–55 years were enrolled in the study. Specimens were obtained from the site of lesion. All the specimens were subjected to histopathological analysis and HPV-16, 18 detection was done using real-time polymerase chain reaction technique. Results: Mean age of patients was 34.00 ± 8.85. Majority were males (62%, from middle social class (78% and were educated up to graduation or above (54%. All the patients had one or more adverse habits (betel, chewing tobacco, smoking and alcohol with mean duration of disease was 3.53 ± 3.05 years. Five patients (10% had diabetes. Burning sensation (86% and trismus (36% were the reported complaints. Maximum were Stage II (50%, sized 2–4 cm2 (60%, homogenous (70%. Floor of mouth and tongue were most commonly involved sites (50%. Majority had mild dysplasia (58%. Acanthosis (66%, hyperplasia (62%, koliocytosis (40%, and keratosis (98% were quite common. HPV (16/18 could not be detected in any specimen. Conclusions: The present study highlighted the fact that association of HPV with oral leukoplakia seems to be overestimated and needs to be reexamined with consensus Human papilloma virus (HPV primers to detect HPV types with more valid empirical relationships.

  17. Warty (condylomatous) squamous cell carcinoma of the penis: a report of 11 cases and proposed classification of 'verruciform' penile tumors.

    Science.gov (United States)

    Cubilla, A L; Velazques, E F; Reuter, V E; Oliva, E; Mihm, M C; Young, R H

    2000-04-01

    Within the spectrum of penile squamous cell carcinomas, those that we descriptively refer to collectively as the "verruciform" lesions are particularly difficult to subclassify. In a review of 50 such tumors, we found 11 distinctive neoplasms with condylomatous features conforming to the appearance of so-called "warty (condylomatous) carcinoma." The average patient age was 55 years and the average duration of disease was 19 months. The primary tumor involved multiple anatomic sites (glans, coronal sulcus, and foreskin) in seven cases and a single site (glans or foreskin) in four cases. Grossly, white to gray cauliflower-like tumors typically measuring approximately 5 cm were noted. Histologically the tumors were mainly papillomatous with acanthosis and hyperkeratosis. The papillae had prominent fibrovascular cores. The most conspicuous microscopic findings were striking nuclear atypia of koilocytotic type and clear cytoplasm. The interface between tumor and stroma was irregular in the majority of cases; deep invasion of corpus cavernosum was noted in five cases. The differential diagnosis included verrucous carcinoma, low-grade papillary squamous cell carcinoma, not otherwise specified, and giant condyloma acuminatum. Among other differences, the first two lesions show no koilocytotic changes and the last lacks malignant features and irregular stromal invasion. Metastatic spread occurred in two patients; both are alive with evidence of recurrent disease 12 and 72 months after initial diagnosis. A third patient was alive with recurrent disease 12 months after diagnosis. Five patients were free of disease 8, 12, 24, 52, and 108 months after diagnosis. Three patients were lost to follow up. Warty (condylomatous) carcinomas of the penis are morphologically distinctive verruciform neoplasms with features of human papillomavirus-related lesions and should be distinguished from other verruciform tumors so that differences in behavior, if any, between these tumors will

  18. A case of multiple lichen planus-like keratoses with junctional melanocytic proliferation%多发性扁平苔藓样角化症伴交界区黑素细胞增生一例

    Institute of Scientific and Technical Information of China (English)

    关杨; 陶小华; 张芸; 莫衍石

    2011-01-01

    报告1例多发性扁平苔藓样角化症伴交界区黑素细胞增生.患者男,83岁.四肢紫红色扁平丘疹、斑块逐渐增多1年,无服药史.组织病理检查显示,表皮角化过度伴角化不全,棘层肥厚,可见坏死的角质形成细胞,基底细胞灶性液化,真皮浅层带状淋巴细胞及少数浆细胞和嗜酸粒细胞浸润,皮损边缘可见日光性黑子样改变.免疫组化染色,苔藓样浸润处Mela-A阳性黑素细胞明显增多,细胞体积大呈星状.%A case of multiple lichen planus-like keratoses with junctional melanocytic proliferation is reported.An 83-year-old male patient complained of multiple purple red flat papules and plaques on the extremities for 1 year with no medication history.Histopathology examination showed epidermal hyperkeratosis,focal parakeratosis, acanthosis, necrosis of keratinocytes, focal liquefaction of basal cells, bandlike infiltration of lymphocytes, eosinophils and few plasma cells in superficial dernis.Solar lentigo-like pathological changes were observed at the edge of the lesions.Immunohistochemical study revealed an obvious increase in the count of large, star-shaped, Mela-A-positive melanocytes in the lichenoid infiltrates.

  19. Clinical and histopathologic study of benign lichenoid keratosis on the face.

    Science.gov (United States)

    Kim, Han Su; Park, Eun Joo; Kwon, In Ho; Kim, Kwang Ho; Kim, Kwang Joong

    2013-10-01

    Benign lichenoid keratosis is a cutaneous entity that consists of a nonpruritic papule or slightly indurated plaque that is histologically characterized by a band-like inflammatory infiltrate with interface involvement. The purpose of this study was to investigate the clinical and histopathologic features of benign lichenoid keratosis localized on the face. Fourteen benign lichenoid keratosis patients diagnosed clinically and histopathologically in our clinic during the 10-year period from 2002 to 2012 were studied. Thirteen female and 1 male patients were included. The mean age at diagnosis was 46.5 years. The color of most of the lesions was brown (10 cases, 71%). The cheek was the most commonly involved area (10 cases, 71%). All of the lesions were single. There were 9 (64%) flat lesion cases and 5 (36%) raised lesion cases. Most patients denied having any symptoms; 3 had mild pruritus. The histopathological findings indicated that all the cases exhibited lichenoid inflammatory infiltrate obscuring the dermal-epidermal junction and vacuolar alteration of basal cell layer. The lesions showed focal parakeratosis (79%), melanophages (79%), hyperkeratosis (71%), and necrotic keratinocytes (71%). Solar elastosis (50%) and acanthosis (43%) were also seen frequently. Diagnosis of benign lichenoid keratosis should be made by a combination of clinical manifestations and histopathological findings. In particular, benign lichenoid keratosis should be considered if a middle-aged patient presents a solitary asymptomatic brown lesion on the face. We think benign lichenoid keratosis may be a specific disorder rather than the inflammatory stage of regressing solar lentigines, large cell acanthoma or reticulated seborrheic keratosis.

  20. Lichenoid Dermatologic Toxicity From Immune Checkpoint Blockade Therapy: A Detailed Examination of the Clinicopathologic Features.

    Science.gov (United States)

    Tetzlaff, Michael T; Nagarajan, Priyadharsini; Chon, Susan; Huen, Auris; Diab, Adi; Omar, Pacha; Aung, Phyu P; Torres-Cabala, Carlos A; Mays, Steven R; Prieto, Victor G; Curry, Jonathan L

    2017-02-01

    Immunotherapy targeting the programmed cell death 1 (PD-1) receptor has demonstrated tremendous promise in the treatment of advanced solid tumors. Dermatologic toxicities, however, are an emerging consequence of this therapy and have been clearly associated with immune checkpoint blockade antibodies. Distinctive clinical and histologic subtypes of dermatologic toxicity secondary to immunotherapy are emerging and include rare autoimmune bullous reactions (eg, bullous pemphigoid) and lichenoid eruptions. We report three patients who developed lichenoid dermatitis while receiving anti-PD-1 antibody therapy. The mean time to onset of lichenoid dermatologic toxicity was 42 days (range: 1-75 days) from initiation of anti-PD-1 antibody therapy. Lesions most frequently presented on the extremities and trunk as pustules, papules, and plaques. The face was not commonly involved. Of the five skin biopsies examined, all demonstrated dense band-like lymphocytic infiltrate, hyperkeratosis, hypergranulosis, saw-tooth rete ridge pattern, and dyskeratosis. Acanthosis was a feature in all of the skin biopsies, and in one, epidermal hyperplasia was prominent. In several skin biopsies, histologic features supporting a lichenoid drug eruption were present, including parakeratosis, spongiosis, periadnexal/perivascular inflammation, and eosinophils. Furthermore, the histologic features varied in skin biopsy specimens taken from the same patient at different sites, supporting a drug reaction. All patients' skin lesions improved with use of steroids: two were treated with topical steroids and one with systemic steroids. Recognition of the histopathologic patterns of dermatologic toxicities resulting from immune checkpoint blockade therapy will become increasingly important for ensuring appropriate management of dermatologic toxicities and optimal patient care.

  1. 丘疹性蕈样肉芽肿一例%Papular mycosis fungoides: a case report

    Institute of Scientific and Technical Information of China (English)

    王一玲; 刘泽虎; 陈书悦; 郑俊惠; 乔刚; 沈宏; 许爱娥

    2011-01-01

    患者男,27岁,因左胸部、左腋窝丘疹3年就诊,无自觉症状,皮损持续不退.组织病理检查示棘层轻度增生肥厚,皮突延长,真皮浅层苔藓样淋巴样细胞浸润,部分移人表皮,形成Pautrier微脓肿.免疫组化示LCA(+)、CD45RO(+)、CD3(+)、CD4(+)、CD8散在(+)、CD20(-)、CD68(-),CD30(-).皮肤激光共聚焦显微镜显示棘层中散在有轻度折光的椭圆或圆形细胞影,直径4~8 μm.诊断:丘疹性蕈样肉芽肿.治疗:外用丙酸氟替卡松乳膏、异维A酸凝胶,窄波紫外线照射,丘疹变软,色泽变淡.%A 27-year-old man presented with a 3-year history of persistent asymptomatic papules on the left chest and axirlary fossa. Multiple skin biopsies were performed and histopathology revealed mild acanthosis, extension of the dermal papilla, lichenoid lymphoid infiltrates in upper dermis. Some lymphoid cells migrated into the epidermis and formed Pautrier's microabscesses. Immunohistochemistry revealed that the infiltrating cells were positive for LCA, CD45RO, CD3, CD4 and CD8 (scattered), but negative for CD68 or CD30. Cutaneous laser confocal microscopy showed the shadow of scattered, oval or round, slightly refractive cells measuring 4-8 pm in diameter. A diagnosis of papular mycosis fungoides was made. The papules were softened with the lightening of lesional color after treatment with narrow-band ultraviolet B, topical fluticasone propionate cream and isotretinoin gel.

  2. CLINICOPATHOLOGICAL STUDY OF LICHENOID REACTIONS: A RETROSPECTIVE ANALYSIS

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    Ravikant

    2015-04-01

    Full Text Available BACKGROUND: Lichenoid dermatose s refer to various, clinically different inflammatory dermatoses which share in common, various essential lichenoid histologic features. AIMS AND OBJECTIVE: In this study we have analysed the different clinicopathological aspects in lichenoid reactions, as prompt diagnosis of these lesions can greatly influence the morbidity associated with the disease. STUDY DESIGN: This was a 14 month retrospective analysis of cases presenting to the department of dermatology from June 2013 till July 2014. MATERIALS AND METHODS: All new patients diagnosed with lichenoid reaction clinically and histopathologically were included in the study. INCLUSION CRITERIA: All newly diagnosed cases of Lichenoid tissue reactions within the time period mentioned above . EXCLUSION CRITERI A: Patients whose adequate records were unavailable Statistical Analysis: The SPSS, version 13 software was used to statistically analyse the data. RESULTS: A total of 66 cases were diagnosed as lichenoid reactions, 51 were lichen planus and 15 had licheno id eruptions. Males (53% were more commonly affected than female s (47%, with lower limbs (69. 69% being the most common site of predilection. Oral lesions were seen among eleven patients. Histopathologically, the most consistent findings with lichenoid r eaction was the presence of a lymphocytic infiltrate followed by vacuolar degeneration of basal cell layer, hyperkeratosis, hypergranulosis, acanthosis, pigment incontinence and a band like infiltrate over the basal layer. CONCLUSION: In our study, Licheno id reactions were more common among adults from the 2 nd to 4 th decade and can show ed a male preponderance, with the most frequent archetype being lichen planus. Thus this study emphasises on the need of histological analysis in various clinically similar c ases of lichenoid dermatoses in order to arrive at a definitive diagnosis.

  3. Zinc-responsive acral hyperkeratotic dermatosis-A novel entity or a subset of some well-known dermatosis?

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    Arghyaprasun Ghosh

    2015-01-01

    Full Text Available Background: We are reporting a series of interesting cases, which presented to us with psoriasiform lesions distributed over the acral regions of the body. The cases are unusual because they were resistant to conventional treatment modalities like topical corticosteroids, tacrolimus and oral methotrexate but showed significant improvement on oral zinc therapy. Materials and Methods: Ten patients with characteristic clinical features of distinctive hyperkeratotic plaque in the acral areas, who were resistant to treatment by different modalities including potent topical steroids and oral methotrexate, were included for detailed investigations. A proper history was taken and relevant laboratory investigations were done which included blood count, urine, liver function, renal function, hepatitis-C virus serology and serum zinc levels. Patients were followed up every 2 weeks. Histopathological examinations of the lesional tissue were done at baseline and after 6 weeks of therapy. Patients were given oral zinc daily and no other treatment during the 6 weeks course. Results: All our patients were non-reactive to hepatitis-C. Of the ten patients only one patient (10% showed low titer of serum zinc, another (10% showed higher zinc level, while the rest of the patients had normal zinc level. Five of our patients had chronic renal failure, one had Grave′s disease and the remaining had no associated systemic illness. Histopathology mostly showed hyperkeratosis, acanthosis, prominent granular layer, spongiosis and dermal infiltrate. After 6 weeks of follow up, all patients showed rapid and remarkable therapeutic response with zinc. Conclusions: We here report a series of patients, discernible because of their uniform clinical presentation of acral hypekeratotic plaques and in showing a noticeable response to zinc. Clinical, histopathological and laboratory investigations were done to rule out diseases of similar morphology including psoriasis, acral

  4. NTP Toxicology and Carcinogenesis Studies of Diethanolamine (CAS No. 111-42-2) in F344/N Rats and B6C3F1 Mice (Dermal Studies).

    Science.gov (United States)

    1999-07-01

    Diethanolamine is widely used in the preparation of diethanolamides and diethanolamine salts of long-chain fatty acids that are formulated into soaps and surfactants used in liquid laundry and dishwashing detergents, cosmetics, shampoos, and hair conditioners. Diethanolamine is also used in textile processing, in industrial gas purification to remove acid gases, as an anticorrosion agent in metalworking fluids, and in preparations of agricultural chemicals. Aqueous diethanolamine solutions are used as solvents for numerous drugs that are administered intravenously. Diethanolamine was selected for evaluation because its large-scale production and pattern of use indicate the potential for widespread human exposure. Male and female F344/N rats and B6C3F1 mice received dermal applications of diethanolamine in 95% ethanol for 2 years. Genetic toxicology studies were performed in Salmonella typhimurium, L5178Y mouse lymphoma cells, cultured Chinese hamster ovary cells, and B6C3F1 mouse peripheral blood erythrocytes. RATS: Groups of 50 male rats were administered 0, 16, 32, or 64 mg diethanolamine/kg body weight in ethanol dermally for 2 years. Groups of 50 female rats were administered 0, 8, 16, or 32 mg/kg in ethanol dermally for 2 years. Survival, Body Weights, and Clinical Findings Survival of vehicle control and dosed male and female rats was similar. Mean body weights of 64 mg/kg males were less than those of the vehicle controls beginning week 8, and mean body weights of females were generally similar to those of the vehicle control group. The only clinical finding attributed to diethanolamine administration was irritation of the skin at the site of application. Pathology Findings: Minimal to mild nonneoplastic lesions occurred at the site of application in the epidermis of dosed male and female rats. The incidence of acanthosis in 64 mg/kg males, the incidences of hyperkeratosis in 32 and 64 mg/kg males and in all dosed female groups, and the incidences of exudate

  5. 多菌种两步法联合发酵饲料的研究%Multi-strain co-fermented feed by two-step method

    Institute of Scientific and Technical Information of China (English)

    刘桂香; 史瑛; 赵建新; 张灏; 陈卫

    2016-01-01

    试验研究了利用米曲霉、根霉、啤酒酵母、枯草芽孢杆菌、植物乳杆菌两步法联合发酵饲料的最佳工艺条件。试验结果显示,发酵饲料的最佳接种量为霉菌(黑根霉∶米曲米为1∶1)1%、枯草芽孢杆菌1%、啤酒酵母6%、植物乳杆菌1%;最佳料水比为1∶0.7;最佳工艺为在发酵初始将所有菌种接种到灭菌饲料中,在30℃下保温保湿发酵24 h,然后升温至37℃继续通风发酵24 h,封袋,进入厌氧发酵阶段,25℃发酵6 d。最终发酵饲料粗蛋白质质量分数为32%,比发酵前增加了59%,α-淀粉酶活力103 U/g,蛋白酶活力123 U/g,乳酸菌为4.7×1011 CFU/g,枯草芽孢杆菌为1.3×1010 CFU/g,酵母菌为1.8×104 CFU/g,为富蛋白质、富益生菌以及高酶活性的芳香适口的饲料。%The optimum process conditions of multi-strain(Aspergillus oryzae ,Rhizopus nigricans ,Saccharomyces cerevisi-ae ,Bacillus subtilis and Lactobacillus )co-fermented feed by two-step method were studied.The experiment results showed that the optimal inoculation amount of mould(Aspergillus oryzae ∶Rhizopus nigricans =1∶1)was 1%,Saccharomyces cere-visiae 1%,Bacillus subtilis 1% and Lactobacillus 1%.The ratio of material to water was 1∶0.7.All kinds of strains were in-oculumed to the sterile steamed feed at the beginning of fermention,and were kept at 30℃ for 12 h,then at 37℃ for ventilation fermentation for 24 h,and then sealed up for anaerobic fermentation,and kept at 25℃ for 6 days.The crude protein content of the fermented feed increased by 59% and reached 32%.The α-amylase activity reached 103 U/g and protease activity reached 123 U/g.The number of Lactobacillus reached 4.7×10 1 1 CFU/g,Bacillus subtilis reached 1.3 ×10 1 0 CFU/g and Saccharo-myces cerevisiae reached 1.8×10 4 CFU/g.The product exbihited the characteristics of both protein feed and probiotics and en-zyme activity with aromatherapy scent and good palatability.

  6. [Concentration of allergic fungi spores in the air of flats in Lódź].

    Science.gov (United States)

    Krawczyk, P; Kowalski, M L; Ochecka-Szymańska, A

    1999-01-01

    The real contribution of moulds to the pathogenesis of allergic diseases remains unknown, although positive skin prick tests and/or specific serum IgE to moki allergens can be detected in 1-5% of atopic patients. A significant problem in assesment of exposure to mould allergens, resulting with difficulty in standarization of methods. The aim of this work was to assess the concentration of spores of 8 mould species in flats inhabited by peoples who Bont show any symptoms of allergy. The Open Petri Dish (OPD) method involving sedimentation of participles contained in the column of air over the dish was used to assess the number of spores in 1 m3 of indoor atmospheres. All colonies were counted, but only 8 mould species implicated in inhaled allergy were identified, ie.: Alternaria tenuis, Cladosporium herbarum, Helminthosporum halodes, Pullularia pullulans, Penicillium notatam, Rhizopus nigricans, Mucor mucedo, Aspergillus fumigatus. The tests were carried out in 10 flats located in various quarters of the cify of Lodź during three consecutive days of September 1995 between 5:00 pm and 6:04 pm. In analyzing the percentage of spores of each of the eight mould species tested we determined that, independent of fiat and test day, C. herbarum predominated. It is good agreement with the observations of other authors who report that among large quantities of fungi that are detected in late summer, usually C. herbarum spores dominate. This is the season when the incidence of the Cladosporium spores in the atmospheric air increases. Spores of H. halodes were detected least frequently. Our study demonstrated the presence of substantial amounts of mould spores in indoor air of houses in Lódź. The spores belong to species with documented allergenicity, suggesting that they may play a role in development of allergic sensitization in susceptible subjects.

  7. Reconstruction of historic sea ice conditions in a sub-Arctic lagoon

    Science.gov (United States)

    Petrich, Chris; Tivy, Adrienne C.; Ward, David H.

    2014-01-01

    Historical sea ice conditions were reconstructed for Izembek Lagoon, Bering Sea, Alaska. This lagoon is a crucial staging area during migration for numerous species of avian migrants and a major eelgrass (Zostera marina) area important to a variety of marine and terrestrial organisms, especially Pacific Flyway black brant geese (Branta bernicla nigricans). Ice cover is a common feature of the lagoon in winter, but appears to be declining, which has implications for eelgrass distribution and abundance, and its use by wildlife. We evaluated ice conditions from a model based on degree days, calibrated to satellite observations, to estimate distribution and long-term trends in ice conditions in Izembek Lagoon. Model results compared favorably with ground observations and 26 years of satellite data, allowing ice conditions to be reconstructed back to 1943. Specifically, periods of significant (limited access to eelgrass areas) and severe (almost complete ice coverage of the lagoon) ice conditions could be identified. The number of days of severe ice within a single season ranged from 0 (e.g., 2001) to ≥ 67 (e.g., 2000). We detected a slight long-term negative trend in ice conditions, superimposed on high inter-annual variability in seasonal aggregate ice conditions. Based on reconstructed ice conditions, the seasonally cumulative number of significant or severe ice days correlated linearly with mean air temperature from January until March. Further, air temperature at Izembek Lagoon was correlated with wind direction, suggesting that ice conditions in Izembek Lagoon were associated with synoptic-scale weather patterns. Methods employed in this analysis may be transferable to other coastal locations in the Arctic.

  8. Lipodistrofia parcial familiar tipo 1: ¿Un síndrome subdiagnosticado o raro?

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    Jimena Soutelo

    2015-02-01

    Full Text Available La lipodistrofia parcial familiar de tipo1 (LPF 1 es un síndrome caracterizado por la pérdida parcial de grasa subcutánea en extremidades con distribución incrementada de la misma en rostro, cuello y tronco. Es una identidad familiar aunque hay casos espontáneos. Hasta ahora no se conoce mutación responsable. Se debe realizar diagnóstico diferencial con el síndrome de Cushing. Es un síndrome poco frecuente y en oportunidades se llega al diagnóstico cuando los pacientes presentan complicaciones cardiovasculares o afectación pancreática como consecuencia de una grave alteración metabólica. Se presenta el caso de una paciente de 45 años con diabetes mellitus desde los 20 años de edad, mal control glucémico (HbA1c: 11.7% e hipertrigliceridemia (TG: 3000 mg/dl, índice de masa corporal (IMC: 38, extremidades adelgazadas, pérdida de grasa subcutánea en glúteos, sobreelevación de pliegue por encima de los mismos, venas prominentes en miembros inferiores, cara de luna llena y marcada acantosis nigricans, hipertensión (TA: 150/100 mm Hg y medidas de pliegues subcutáneos disminuidos. El dosaje de leptina fue 16.8 mg/ml. El estudio genético para gen LMNA fue negativo. Se instauraron medidas de cambio de estilo de vida, tratamiento con fenofibrato, insulina premezcla 50/50 y enalapril, obteniéndose una franca mejoría clínica, de la HbA1c (7.8% y de los TG (243 mg/dl.

  9. Effects of El Niño on distribution and reproductive performance of Black Brant

    Science.gov (United States)

    Sedinger, J.S.; Ward, D.H.; Schamber, J.L.; Butler, W.I.; Eldridge, W.D.; Conant, B.; Voelzer, J.F.; Chelgren, N.D.; Herzog, M.P.

    2006-01-01

    Climate in low-latitude wintering areas may influence temperate and high-latitude breeding populations of birds, but demonstrations of such relationships have been rare because of difficulties in linking wintering with breeding populations. We used long-term aerial surveys in Mexican wintering areas and breeding areas in Alaska, USA, to assess numbers of Black Brant (Branta bernicla nigricans; hereafter brant) on their principal wintering and breeding area in El Niño and non-El Niño years. We used Pollock's robust design to directly estimate probability of breeding and apparent annual survival of individually marked brant at the Tutakoke River (TR) colony, Alaska, in each year between 1988 and 2001. Fewer brant wintered in Mexico during every El Niño event since 1965. Fewer brant were observed on the principal breeding area following each El Niño since surveys began in 1985. Probability of breeding was negatively related to January sea surface temperature along the subtropical coast of North America during the preceding winter. Between 23% (five-year-olds or older) and 30% (three-year-olds) fewer brant nested in 1998 following the strong El Niño event in the winter of 1997–1998 than in non-El Niño years. This finding is consistent with life history theory, which predicts that longer-lived species preserve adult survival at the expense of reproduction. Oceanographic conditions off Baja California, apparently by their effect on Zostera marina (eelgrass), strongly influence winter distribution of brant geese and their reproduction (but not survival), which in turn affects ecosystem dynamics in Alaska.

  10. Tracing carbon flow through coral reef food webs using a compound-specific stable isotope approach

    KAUST Repository

    McMahon, Kelton

    2015-11-21

    Coral reefs support spectacularly productive and diverse communities in tropical and sub-tropical waters throughout the world’s oceans. Debate continues, however, on the degree to which reef biomass is supported by new water column production, benthic primary production, and recycled detrital carbon (C). We coupled compound-specific stable C isotope ratio (δ13C) analyses with Bayesian mixing models to quantify C flow from primary producers to coral reef fishes across multiple feeding guilds and trophic positions in the Red Sea. Analyses of reef fishes with putative diets composed primarily of zooplankton (Amblyglyphidodon indicus), benthic macroalgae (Stegastes nigricans), reef-associated detritus (Ctenochaetus striatus), and coral tissue (Chaetodon trifascialis) confirmed that δ13C values of essential amino acids from all baseline C sources were both isotopically diagnostic and accurately recorded in consumer tissues. While all four source end-members contributed to the production of coral reef fishes in our study, a single-source end-member often dominated dietary C assimilation of a given species, even for highly mobile, generalist top predators. Microbially reworked detritus was an important secondary C source for most species. Seascape configuration played an important role in structuring resource utilization patterns. For instance, Lutjanus ehrenbergii showed a significant shift from a benthic macroalgal food web on shelf reefs (71 ± 13 % of dietary C) to a phytoplankton-based food web (72 ± 11 %) on oceanic reefs. Our work provides insights into the roles that diverse C sources play in the structure and function of coral reef ecosystems and illustrates a powerful fingerprinting method to develop and test nutritional frameworks for understanding resource utilization.

  11. Characterization of free nitrogen fixing bacteria of the genus Azotobacter in organic vegetable-grown Colombian soils

    Directory of Open Access Journals (Sweden)

    Diego Javier Jiménez

    2011-09-01

    Full Text Available With the purpose of isolating and characterizing free nitrogen fixing bacteria (FNFB of the genus Azotobacter, soil samples were collected randomly from different vegetable organic cultures with neutral pH in different zones of Boyacá-Colombia. Isolations were done in selective free nitrogen Ashby-Sucrose agar obtaining a recovery of 40%. Twenty four isolates were evaluated for colony and cellular morphology, pigment production and metabolic activities. Molecular characterization was carried out using amplified ribosomal DNA restriction analysis (ARDRA. After digestion of 16S rDNA Y1-Y3 PCR products (1487pb with AluI, HpaII and RsaI endonucleases, a polymorphism of 16% was obtained. Cluster analysis showed three main groups based on DNA fingerprints. Comparison between ribotypes generated by isolates and in silico restriction of 16S rDNA partial sequences with same restriction enzymes was done with Gen Workbench v.2.2.4 software. Nevertheless, Y1-Y2 PCR products were analysed using BLASTn. Isolate C5T from tomato (Lycopersicon esculentum grown soils presented the same in silico restriction patterns with A. chroococcum (AY353708 and 99% of similarity with the same sequence. Isolate C5CO from cauliflower (Brassica oleracea var. botrytis grown soils showed black pigmentation in Ashby-Benzoate agar and high similarity (91% with A. nigricans (AB175651 sequence. In this work we demonstrated the utility of molecular techniques and bioinformatics tools as a support to conventional techniques in characterization of the genus Azotobacter from vegetable-grown soils.

  12. 240 Mold Sensitization in Chronic Rhinosinusitis Patients

    Science.gov (United States)

    Gawlik, Radoslaw; Czecior, Eugeniusz

    2012-01-01

    Background It is estimated that about 10% of the population have IgE antibodies to common inhalant molds. Exposure to fungal allergens could be linked to the presence and persistence of asthma, rhinitis and atopic dermatitis. Mold sensitization is a risk factor for development and deterioration of upper airway allergy, especially chronic rhinosinusitis. We addressed the incidence of mold allergy measured as specific IgE to molds and skin prick tests in chronic sinusitis patients. We assessed prevalence of allergic reactions to mould among surgery treated chronic sinusitis patients. Methods A group of 28 chronic sinusitis patients after surgery were included into the study. Routine medical examination, skin prick tests with common inhaled allergens and extended mold panel (Alternaria alternate, Cladosporium herbarium, Aspergilus fumigatus, Candida albicans, Mucor mucedo, Botrytis cinerea, Rhisopus nigricans, Penicilliumi notatum, Fusarum moniliforme Pullularia pullulans (Allergopharma, Germany), tIgE, asIgE measurement were performed (Phadia, Sweden). All investigated patients were consulted by laryngologist and mycological examination was performed. Results We found that sensitization to at least one allergen was present in 43.8(14/32) of sinusitis patients. The most prevalent was sensitization to house dust mite Dermatophagoides pt., found in 21.8 % (7/32) patients. Positive results of skin prick tests with Candida albicans we observed in 18.8% (6/32), with Alternaria alternate in 15,6% (5/32), Cladosporium herbarium in 6,3% (2/32), Aspergilus fumigatus in 3,13 % (1/32). None of investigated patients presented sensitization to other mold allergens. Microbiological methods demonstrated fungal infection only in 2 patients. Conclusions Almost half of chronic sinusitis patients presented sensitization to at least one allergen. Fungal allergy is relatively rare in chronic sinusitis patients.

  13. O que é melhor para manter a riqueza de espécies de morcegos (Mammalia, Chiroptera: um fragmento florestal grande ou vários fragmentos de pequeno tamanho? What is better for maintaining the richness of bat (Mammalia, Chiroptera species: a large forest fragment or many small fragments?

    Directory of Open Access Journals (Sweden)

    Nelio Roberto dos Reis

    2003-06-01

    Full Text Available This study was carried out with the objective of evaluating if the size of forest fragments affects the diversity of bat species. In order to do that, seven fragments were studied in Londrina, Paraná: five small fragments, whose areas varied between 1 and 10 ha; a fragment which is considered medium-sized (Parque Municipal Arthur Thomas - 85,47 ha.; and a large fragment (Parque Estadual Mata dos Godoy - 680 ha.. Thirty three species were collected. Ten species were common to all three types of fragments: Chrotopterus auritus (Peters, 1856, Carollia perspicillata (Linnaeus, 1758, Artibeus fimbriatus Gray, 1838, Artibeus lituratus (Olfers, 1818, Platyrrhinus lineatus (E. Geoffroy, 1810, Pygoderma bilabiatum (Wagner, 1843, Sturnira lilium (E. Geoffroy, 1810, Vampyressa pusilla (Wagner, 1843, Eptesicus brasiliensis (Desmarest, 1819, and Myotis nigricans (Schinz, 1821. Eight species were only found in the large fragment: Noctilio albiventris Desmarest, 1818, Glossophaga soricina (Pallas, 1766, Uroderma bilobatum Peters, 1866, Diaemus youngi (Jentink,1893, Diphylla ecaudata Spix, 1823, Eptesicus furinalis (d'Orbigny, 1847, Histiotus velatus (I. Geoffroy, 1824 and Myotis levis (I. Geoffroy, 1824. Five were only found in the small fragments: Noctilio leporinus (Linnaeus, 1758, Phyllostomus discolor Wagner, 1843, Chiroderma villosum Peters, 1860, Eptesicus sp. e Rogheessa tumida H. Allen, 1866. Chiroderma doriae, which is threatened by extinction, was captured in the large fragment and in one of the small fragments; M. ruber, also threatened by extinction, was captured in the medium-sized and large fragments. We believe that the major cause for the loss of organic diversity is not rational exploitation, but the destruction of habitats, a result of the expansion of irrational human activities.

  14. Bat-species richness in the Pantanal floodplain and its surrounding uplands.

    Science.gov (United States)

    Alho, C J R; Fischer, E; Oliveira-Pissini, L F; Santos, C F

    2011-04-01

    We studied the bat fauna of the Pantanal floodplain and its surrounding plateaus in Mato Grosso do Sul, Brazil, based on the scientific collection at Universidade Anhanguera-Uniderp and on the Projeto Morcegos do Pantanal data bank at UFMS, comprising 9,037 captures of 56 species recorded from 1994 to 2007. The Pantanal surveys were carried out in the Nhecolândia, Aquidauana, Miranda, and Paraguai sub-regions; the uplands surveys took place in the Maracaju, Bodoquena, and Urucum formations. Bat specimens were mist-netted over 376 nights in 35 sites, predominantly near fruiting trees, bat shelters, and forest patches. In the floodplain 46 species were recorded (n = 6,292 individuals), and 44 species were found in the uplands (n = 2,745 individuals). Six families were recorded: Phyllostomidae (30 species), Molossidae (12 species), Verpertilionidae (nine species) Noctilionidae (two species), Emballorunidae (two species) and Mormoopidae (one species). The bat fauna was predominantly composed of insectivore (32) and frugivore (15) species. The frugivorous Artibeus planirostris (n = 3,101 individuals) was the commonest species in floodplain and uplands. Other common species were Myotis nigricans (n = 762), Molossus molossus (n = 692), Noctilio albiventris (n = 681), Platyrrhinus lineatus (n = 633), Sturnira lilium (n = 461), Carollia perspicillata (n = 451), Glossophaga soricina (n = 436), Artibeus lituratus (n = 320), and Desmodus rotundus (n = 281). In the floodplain there were three insectivores among the most common species, contrasting with the uplands dominated by the frugivores. The diversity for the 35 sites assembled (H' = 2.5) is comparable to that recorded for tropical forests. The bat fauna presented here represents 34% of the Brazilian bat species, and 62% of species reported for the Upper Paraguay River Basin. Additionally, five species are reported for the first time in Mato Grosso do Sul.

  15. Can Homeopathy Bring Additional Benefits to Thalassemic Patients on Hydroxyurea Therapy? Encouraging Results of a Preliminary Study

    Directory of Open Access Journals (Sweden)

    Antara Banerjee

    2010-01-01

    Full Text Available Several homeopathic remedies, namely, Pulsatilla Nigricans (30th potency, Ceanothus Americanus (both mother tincture and 6th potency and Ferrum Metallicum (30th potency selected as per similia principles were administered to 38 thalassemic patients receiving Hydroxyurea (HU therapy for a varying period of time. Levels of serum ferritin (SF, fetal hemoglobin (HbF, hemoglobin (Hb, platelet count (PC, mean corpuscular volume (MCV, mean corpuscular hemoglobin concentration (MCHC, mean corpuscular hemoglobin (MCH, white blood cell (WBC count, bilirubin content, alanine amino transferase (ALT, aspartate amino transferase (AST and serum total protein content of patients were determined before and 3 months after administration of the homeopathic remedies in combination with HU to evaluate additional benefits, if any, derived by the homeopathic remedies, by comparing the data with those of 38 subjects receiving only HU therapy. Preliminary results indicated that there was a significant decrease in the SF and increase in HbF levels in the combined, treated subjects. Although the changes in other parameters were not so significant, there was a significant decrease in size of spleen in most patients with spleenomegaly and improvement in general health conditions along with an increased gap between transfusions in most patients receiving the combined homeopathic treatment. The homeopathic remedies being inexpensive and without any known side-effects seem to have great potentials in bringing additional benefits to thalassemic patients; particularly in the developing world where blood transfusions suffer from inadequate screening and fall short of the stringent safety standards followed in the developed countries. Further independent studies are encouraged.

  16. The effect of vitamin D replacement therapy on insulin resistance and androgen levels in women with polycystic ovary syndrome.

    Science.gov (United States)

    Selimoglu, H; Duran, C; Kiyici, S; Ersoy, C; Guclu, M; Ozkaya, G; Tuncel, E; Erturk, E; Imamoglu, S

    2010-04-01

    Insulin resistance (IR) is one of the common features of the polycystic ovary syndrome (PCOS), and recent studies indicate the possible role of vitamin D in the pathogenesis of IR and glucose metabolism. Aim of this study was aimed to determine the effect of vitamin D replacement therapy on glucose metabolism, insulin, and androgen levels in obese, insulin-resistant women with PCOS. Eleven women with PCOS were included in the study. Mean age of the patients was 23.6+/-5.7 yr, body mass index 33.9+/-5.1 kg/m(2). Six patients (54.5%) had acantosis nigricans and 10 (90.9%) oligoamenorrhea. The mean Ferriman Gallwey score was 14.1+/-4.6. Only 2 women were within the normal limits of vitamin D levels as >20 ng/ml. Three weeks after the administration of the single dose of 300,000 units of vitamin D3 orally, 25-hydroxyvitamin D3 significantly increased from 16.9+/-16 ng/ml to 37.1+/-14.6 ng/ml (p: 0.027) and only 2 women were detected to have vitamin D3 levels <20 ng/ml. Although glucose and insulin levels were decreased non-significantly, homeostasis model assessment (HOMA)-IR significantly decreased from 4.41+/-1.38 to 3.67+/-1.48 (p: 0.043). No significant alterations were witnessed at the levels of DHEAS, total and free testosterone, androstenedione. No correlation was found between vitamin D with HOMA and other hormonal parameters. In conclusion, women with PCOS have mostly insufficient vitamin D levels, and vitamin D replacement therapy may have a beneficial effect on IR in obese women with PCOS.

  17. Mercury in tunas and blue marlin in the North Pacific Ocean.

    Science.gov (United States)

    Drevnick, Paul E; Brooks, Barbara A

    2017-03-06

    Models and data from the North Pacific Ocean indicate that mercury concentrations in water and biota are increasing in response to (global or hemispheric) anthropogenic mercury releases. In the present study, we provide an updated record of mercury in yellowfin tuna (Thunnus albacares) caught near Hawaii that confirms an earlier conclusion that mercury concentrations in these fish are increasing at a rate similar to that observed in waters shallower than 1000 m. We also compiled and reanalyzed data from bigeye tuna (Thunnus obesus) and blue marlin (Makaira nigricans) caught near Hawaii in the 1970s and 2000s. Increases in mercury concentrations in bigeye tuna are consistent with the trend found in yellowfin tuna, in both timing and magnitude. The data available for blue marlin do not allow for a fair comparison among years, because mercury concentrations differ between sexes for this species, and sex was identified (or reported) in only 3 of 7 studies. Also, mercury concentrations in blue marlin may be insensitive to modest changes in mercury exposure, because this species appears to have the ability to detoxify mercury. The North Pacific Ocean is a region of both relatively high rates of atmospheric mercury deposition and capture fisheries production. Other data sets that allow temporal comparisons in mercury concentrations, such as pacific cod (Gadus macrocephalus) in Alaskan waters and albacore tuna (Thunnus alalunga) off the US Pacific coast, should be explored further, to aid in understanding human health and ecological risks and to develop additional baseline knowledge for assessing changes in a region expected to respond strongly to reductions in anthropogenic mercury emissions. Environ Toxicol Chem 2016;9999:1-10. © 2017 SETAC.

  18. Larval assemblages of large and medium-sized pelagic species in the Straits of Florida

    Science.gov (United States)

    Richardson, David E.; Llopiz, Joel K.; Guigand, Cedric M.; Cowen, Robert K.

    2010-07-01

    Critical gaps in our understanding of the distributions, interactions, life histories and preferred habitats of large and medium-size pelagic fishes severely constrain the implementation of ecosystem-based, spatially structured fisheries management approaches. In particular, spawning distributions and the environmental characteristics associated with the early life stages are poorly documented. In this study, we consider the diversity, assemblages, and associated habitat of the larvae of large and medium-sized pelagic species collected during 2 years of monthly surveys across the Straits of Florida. In total, 36 taxa and 14,295 individuals were collected, with the highest diversity occurring during the summer and in the western, frontal region of the Florida Current. Only a few species (e.g. Thunnus obesus, T. alalunga, Tetrapturus pfluegeri) considered for this study were absent. Small scombrids (e.g. T. atlanticus, Katsuwonus pelamis, Auxis spp.) and gempylids dominated the catch and were orders of magnitude more abundant than many of the rare species (e.g. Thunnus thynnus,Kajikia albida). Both constrained (CCA) and unconstrained (NMDS) multivariate analyses revealed a number of species groupings including: (1) a summer Florida edge assemblage (e.g. Auxis spp., Euthynnus alleterattus, Istiophorus platypterus); (2) a summer offshore assemblage (e.g. Makaira nigricans, T. atlanticus, Ruvettus pretiosus, Lampris guttatus); (3) an ubiquitous assemblage (e.g. K. pelamis, Coryphaena hippurus, Xiphias gladius); and (4) a spring/winter assemblage that was widely dispersed in space (e.g. trachipterids). The primary environmental factors associated with these assemblages were sea-surface temperature (highest in summer-early fall), day length (highest in early summer), thermocline depth (shallowest on the Florida side) and fluorescence (highest on the Florida side). Overall, the results of this study provide insights into how a remarkable diversity of pelagic species

  19. Tracing carbon flow through coral reef food webs using a compound-specific stable isotope approach.

    Science.gov (United States)

    McMahon, Kelton W; Thorrold, Simon R; Houghton, Leah A; Berumen, Michael L

    2016-03-01

    Coral reefs support spectacularly productive and diverse communities in tropical and sub-tropical waters throughout the world's oceans. Debate continues, however, on the degree to which reef biomass is supported by new water column production, benthic primary production, and recycled detrital carbon (C). We coupled compound-specific stable C isotope ratio (δ(13)C) analyses with Bayesian mixing models to quantify C flow from primary producers to coral reef fishes across multiple feeding guilds and trophic positions in the Red Sea. Analyses of reef fishes with putative diets composed primarily of zooplankton (Amblyglyphidodon indicus), benthic macroalgae (Stegastes nigricans), reef-associated detritus (Ctenochaetus striatus), and coral tissue (Chaetodon trifascialis) confirmed that δ(13)C values of essential amino acids from all baseline C sources were both isotopically diagnostic and accurately recorded in consumer tissues. While all four source end-members contributed to the production of coral reef fishes in our study, a single-source end-member often dominated dietary C assimilation of a given species, even for highly mobile, generalist top predators. Microbially reworked detritus was an important secondary C source for most species. Seascape configuration played an important role in structuring resource utilization patterns. For instance, Lutjanus ehrenbergii showed a significant shift from a benthic macroalgal food web on shelf reefs (71 ± 13 % of dietary C) to a phytoplankton-based food web (72 ± 11 %) on oceanic reefs. Our work provides insights into the roles that diverse C sources play in the structure and function of coral reef ecosystems and illustrates a powerful fingerprinting method to develop and test nutritional frameworks for understanding resource utilization.

  20. Change in abundance of pacific brant wintering in alaska: evidence of a climate warming effect?

    Science.gov (United States)

    Ward, D.H.; Dau, C.P.; Lee, T.; Sedinger, J.S.; Anderson, B.A.; Hines, J.E.

    2009-01-01

    Winter distribution of Pacific Flyway brant (Branta bernicla nigricans) has shifted northward from lowtemperate areas to sub-Arctic areas over the last 42 years. We assessed the winter abundance and distribution of brant in Alaska to evaluate whether climate warming may be contributing to positive trends in the most northern of the wintering populations. Mean surface air temperatures during winter at the end of the Alaska Peninsula increased about 1??C between 1963 and 2004, resulting in a 23% reduction in freezing degree days and a 34% decline in the number of days when ice cover prevents birds from accessing food resources. Trends in the wintering population fluctuated with states of the Pacific Decadal Oscillation, increasing during positive (warm) phases and decreasing during negative (cold) phases, and this correlation provides support for the hypothesis that growth in the wintering population of brant in Alaska is linked to climate warming. The size of the wintering population was negatively correlated with the number of days of strong northwesterly winds in November, which suggests that the occurrence of tailwinds favorable for migration before the onset of winter was a key factor in whether brant migrated from Alaska or remained there during winter. Winter distribution of brant on the Alaska Peninsula was highly variable and influenced by ice cover, particularly at the heavily used Izembek Lagoon. Observations of previously marked brant indicated that the Alaska wintering population was composed primarily of birds originating from Arctic breeding colonies that appear to be growing. Numbers of brant in Alaska during winter will likely increase as temperatures rise and ice cover decreases at high latitudes in response to climate warming. ?? The Arctic Institute of North America.

  1. Temporal and spatial shifts in habitat use by Black Brant immediately following flightless molt

    Science.gov (United States)

    Lewis, Tyler L.; Flint, Paul L.; Schmutz, Joel A.; Derksen, Dirk V.

    2010-01-01

    Each year thousands of Pacific Black Brant (Branta bernicla nigricans) undergo flightless wing molt in the Teshekpuk Lake Special Area (TLSA), Alaska, in two distinct habitats: inland, freshwater lakes and coastal, brackish wetlands. Brant lose body mass during wing molt and likely must add reserves upon regaining flight to help fuel their 2,500 km migration to autumn staging areas. We characterized movements and habitat use by Brant during post-molt (the period immediately following the recovery of flight) by (1) marking individual Brant with GPS (global positioning system) transmitters, and (2) conducting a series of replicate aerial surveys. Individuals molting in inland habitats promptly abandoned their molt wetland during the post-molt and moved into coastal habitats. Consequently, inland habitats were nearly deserted by early August when Brant had regained flight, a decrease of >5,000 individuals from the flightless period of early July. Conversely, coastal molting Brant largely remained in coastal habitats during the post-molt and many coastal wetlands were occupied by large flocks (>1,000 birds). Our results indicate that inland, freshwater wetlands were less suitable post-molt habitats for Brant, while coastal wetlands were preferred as they transitioned from flightless molt. The immediacy with which Brant vacated inland habitats upon regaining flight suggests that food may be limiting during molt and they are not selecting inland molt sites strictly for food resources, but rather a balance of factors including predator avoidance and acquisition of protein for feather growth. Our data clearly demonstrate that patterns of habitat use by Brant in the TLSA change over the course of the molt season, an important consideration for management of future resource development activities in this area.

  2. Study of biological enzymes' inhibition of filamentous fungi on the Crosslake Bridge ruins%生物酶对跨湖桥遗址丝状真菌抑菌作用的研究

    Institute of Scientific and Technical Information of China (English)

    吴健; 楼卫

    2016-01-01

    确定萧山跨湖桥遗址空气、土壤、字幕墙和独木舟侵蚀丝状真菌的种类,探讨生物酶对侵蚀丝状真菌的有效抑菌浓度,为消除生物危害提供参考依据.采用经典方法对侵蚀丝状真菌进行分离、鉴定,并对生物酶的有效抑菌浓度进行研究.空气中主要的丝状真菌有:黑曲霉(Aspergillus niger)、土曲霉(Aspergillus terreus)、展青霉(Penicillium patulum)和圆弧青霉(Penicillium cyclopium).土壤中主导菌为黑曲霉(Aspergillus niger)、土曲霉(Aspergillus terreus)、聚多曲霉(Aspergillus sydowii)、杂色曲霉(Aspergillus versicolor)、圆弧青霉(Penicillium cyclopium)、展青霉(Penicillium patulum)、黑青霉(Penicillium nigricans)、宛氏拟青霉(Paecilomyces varioti和枝孢霉(Cladosporium).字幕墙侵染菌为杂色曲霉(Aspergillus versicolor).独木舟侵蚀菌为宛氏拟青霉(Paecilomyces varioti).不同的丝状真菌对生物酶的感应性/耐受性有很大不同.单一的几丁质酶对丝状真菌的生长发育有抑制作用,但几丁质酶和葡聚糖酶的复合协同作用更能有效地抑制丝状真菌的生长发育.

  3. Distribution and habitat associations of billfish and swordfish larvae across mesoscale features in the Gulf of Mexico.

    Directory of Open Access Journals (Sweden)

    Jay R Rooker

    Full Text Available Ichthyoplankton surveys were conducted in surface waters of the northern Gulf of Mexico (NGoM over a three-year period (2006-2008 to determine the relative value of this region as early life habitat of sailfish (Istiophorus platypterus, blue marlin (Makaira nigricans, white marlin (Kajikia albida, and swordfish (Xiphias gladius. Sailfish were the dominant billfish collected in summer surveys, and larvae were present at 37.5% of the stations sampled. Blue marlin and white marlin larvae were present at 25.0% and 4.6% of the stations sampled, respectively, while swordfish occurred at 17.2% of the stations. Areas of peak production were detected and maximum density estimates for sailfish (22.09 larvae 1000 m(-2 were significantly higher than the three other species: blue marlin (9.62 larvae 1000 m(-2, white marlin (5.44 larvae 1000 m(-2, and swordfish (4.67 larvae 1000 m(-2. The distribution and abundance of billfish and swordfish larvae varied spatially and temporally, and several environmental variables (sea surface temperature, salinity, sea surface height, distance to the Loop Current, current velocity, water depth, and Sargassum biomass were deemed to be influential variables in generalized additive models (GAMs. Mesoscale features in the NGoM affected the distribution and abundance of billfish and swordfish larvae, with densities typically higher in frontal zones or areas proximal to the Loop Current. Habitat suitability of all four species was strongly linked to physicochemical attributes of the water masses they inhabited, and observed abundance was higher in slope waters with lower sea surface temperature and higher salinity. Our results highlight the value of the NGoM as early life habitat of billfishes and swordfish, and represent valuable baseline data for evaluating anthropogenic effects (i.e., Deepwater Horizon oil spill on the Atlantic billfish and swordfish populations.

  4. Quantification of diagenetic overprint processes deduced from fossil carbonate shells and laboratory-based hydrothermal alteration experiments

    Science.gov (United States)

    Griesshaber, Erika; Casella, Laura; Mavromatis, Vasileios; Dietzel, Martin; Immenhauser, Adrian; Schmahl, Wolfgang

    2016-04-01

    Benthic and nektonic marine biogenic carbonate archives represent the foundation of numerous studies aiming at reconstructions of past climate dynamics and environmental change. However, living organisms are not in thermodynamic equilibrium and create local chemical environments where physiologic processes such as biomineralization takes place. After the death of the organism the former physiologic disequilibrium conditions are not sustained any more and all biological tissues are altered by equilibration according to the surrounding environment: diagenesis. With increasing diagenetic alteration, the biogenic structure and fingerprint fades away and is replaced by inorganic features. Thus, recrystallization of organism-specific microstructure is a clear indicator for diagenetic overprint. Microstructural data, which mirror recrystallization, are of great value for interpreting geochemical proxies for paleo-environment reconstruction. Despite more than a century of research dealing with carbonate diagenesis, many of the controlling processes and factors are only understood in a qualitative manner. One of the main issues is that diagenetically altered carbonates are usually present as the product of a complex preceding diagenetic pathway with an unknown number of intermediate steps. In this contribution we present and discuss laboratory based alteration experiments with the aim to investigate time-series data sets in a controlled manner. We conducted hydrothermal alteration experiments with modern Arctica islandica (bivalvia) and Notosaria nigricans (brachiopoda) in order to mimic diagenetic overprint. We explore first the potential of electron backscattered diffraction (EBSD) measurements together with statistical data evaluation as a tool to quantify diagenetic alteration of carbonate skeletons. Subsequently, we compare microstructural patterns obtained from experimentally altered shell material with those of fossil specimens that have undergone variable degrees of

  5. Microflora en semillas de frijol

    Directory of Open Access Journals (Sweden)

    Jos\\u00E9 B. Membre\\u00F1o

    2001-01-01

    Full Text Available Microflora en semillas de frijol (Phaseolus vulgaris L.. Se estudió la microflora bacteriana presente en semillas de frijol y su relación con Xanthomonas campestris pv. phaseoli (Xcp, en 118 genotipos procedentes de VIDAC-98, INTA- Nicaragua, TARS-USDA e Isabela-P.R. Se utilizaron cinco métodos de aislamiento: semilla desinfectada con hipoclorito de sodio, semilla en caldo nutritivo refrigerada por una hora, dispersión de 0,1 ml de suspensión de semillas en medio sólido, siembra líquida de 1 ml de suspensión y semilla en caldo nutritivo, agitado y refrigerado por 24 horas. Se aislaron 104 colonias amarillas de 41 genotipos. Treinta y seis colonias fueron KOH positivo (Gram negativo, 68 negativo (Gram positivo y 34 hidrolizaron almidón. Las colonias de pigmentación amarilla resultaron no patogénicas bajo condiciones de invernadero. Estas se identificaron con el sistema BIOLOG como: Pantoea agglomerans (25, Xanthomonas campestris (2, Enterobacter agglomerans (2, Sphingomonas paucimobilis (2, Pseudomonas fluorescens y Flavimonas oryzihabitans. En adición, los genotipos portaron colonias con pigmentación distinta a la amarilla. En las pruebas de antagonismo se identificaron colonias con actividad de deoxyribonucleasa y de antibiosis a Xcp. De éstas, 15 colonias inhibieron a Xcp significativamente. Se identificaron los hongos Rhizoctonia solani, Penicillium spp., Fusarium spp., Aspergillus flavus, Rhizopus nigricans y Macrophomina phaseolina en un 52,9 % del total de genotipos evaluados

  6. Inhibition of erythrocytes δ-aminolevulinic acid dehydratase (ALAD) activity in fish from waters affected by lead smelters

    Science.gov (United States)

    Schmitt, Christopher J.; Caldwell, Colleen A.; Olsen, Bill; Serdar, Dave; Coffey, Mike

    2002-01-01

    We assessed the effects on fish of lead (Pb) released to streamsby smelters located in Trail, BC (Canada), E. Helena, MT, Herculaneum, MO, and Glover, MO. Fish were collected by electrofishing from sites located downstream of smelters and from reference sites. Blood from each fish was analyzed for δ-aminolevulinic acid dehydratase (ALAD) activity and hemoglobin (Hb), and samples of blood, liver, or carcass were analyzed for Pb, zinc (Zn), or both. Fish collected downstreamof all four smelters sites had elevated Pb concentrations, decreased ALAD activity, or both relative to their respectivereference sites. At E. Helena, fish from the downstream site also had lower Hb concentrations than fish from upstream. Differences among taxa were also apparent. Consistent with previous studies, ALAD activity in catostomids (Pisces: Catostomidae-northern hog sucker,Hypentelium nigricans;river carpsucker, Carpiodes carpio; largescale sucker, Catostomus macrocheilus; and mountain sucker, C. platyrhynchus) seemed more sensitive to Pb-induced ALADinhibition than the salmonids (Pisces: Salmonidae-rainbow trout,Oncorhynchus mykiss; brook trout,Salvelinus fontinalis) or common carp (Cyprinus carpio). Some of these differences may have resulted from differential accumulation of Zn, which was not measured at all sites. We detected noALAD activity in channel catfish (Ictaluruspunctatus) from either site on the Mississippi River at Herculaneum, MO. Our findings confirmed that Pb is releasedto aquatic ecosystems by smelters and accumulated by fish, andwe documented potentially adverse effects of Pb in fish. We recommend that Zn be measured along with Pb when ALAD activityis used as a biomarker and the collection of at least 10 fish ofa species at each site to facilitate statistical analysis.

  7. Pseudoepitheliomatous hyperplasia - an unusual reaction following tattoo: report of a case and review of the literature.

    Science.gov (United States)

    Cui, Wei; McGregor, Douglas H; Stark, Steven P; Ulusarac, Ozlem; Mathur, Sharad C

    2007-07-01

    A 59-year-old woman presented with an itchy and uncomfortable raised lesion at a tattoo site (Fig. 1) on the lateral aspect of the left leg, just above the ankle. The tattoo had been placed 2 years before her presentation and the tattoo site was sun exposed. Immediately after she had the tattoo, she noticed redness of the skin. After a week, a pruritic and red scaly nodule developed that continued to gradually enlarge until her presentation. The patient had tried topical vitamin A and D ointment with no relief. The patient also had tattoos on the arms without any noticeable skin changes. The patient reported that the tattoo procedure on her leg was more painful than that on her arms, and was performed by a different (and perhaps inexperienced) tattoo artist. The original tattoo contained red, green, and yellow pigments. A diagnosis of tattoo granuloma was considered; squamous cell carcinoma and fungal infection were included in the differential diagnosis. A punch biopsy was performed, followed by complete surgical excision of the lesion with a split-thickness skin graft from the right thigh. The skin excision specimen showed a 3 x 2.5-cm granular and pitted pink lesion with well-demarcated, somewhat irregular borders. The lesion was raised 0.5 cm above the skin surface. The lesion was present in the center of the original tattoo. Portions of the original tattoo with green and blue-green pigmentation were visible on either side of the lesion. No satellite lesions were identified. Microscopically, the raised lesion demonstrated striking pseudoepitheliomatous hyperplasia, with irregular acanthosis of the epidermis and follicular infundibula, hyperkeratosis, and parakeratosis (Fig. 2). Follicular plugging was present with keratin-filled cystic spaces. There was a brisk mononuclear inflammatory infiltrate in the dermis, composed primarily of lymphocytes, with admixed plasma cells and histiocytes. Giant cells were occasionally identified. Dermal pigment deposition was

  8. Schimmelpenning syndrome: a case report%Schimmelpenning综合征一例

    Institute of Scientific and Technical Information of China (English)

    王焱; 冯广东; 王晶; 陈浩; 张倩; 武新英; 薛昌敖; 方方; 孙建方

    2016-01-01

    A 17-year-old female patient presented with generalized brown verrucous proliferative skin lesions on the right side of the head,face and neck for 17 years.Ophthalmoscopy showed a hard cystic mass in the right lacrimal sac area with absence of right lower lacrimal punctum.Magnetic resonance imaging of the orbit revealed a quasi-circular mass compressing the ethmoid sinus and extraocular muscles in the right lacrimal sac area.Histopathological examination of skin lesions of the head and face revealed epidermal hyperkeratosis,acanthosis,papillomatous hyperplasia in the epidermis,numerous mature or immature sebaceous glands in the dermis,and ectopic apocrine sweat glands in some areas.A pathological diagnosis of naevus sebaceous was made.Verrucous vegetation was observed in the oral mucosa including the right side of the palate and tongue,and orthopanotomography showed poor development of the right side of upper and lower jaw bones as well as alveolar bones.Moreover,there were large areas of coffee-colored patches and brown spots on both upper extremities.The patient had average intelligence and a history of convulsion.She was diagnosed with Schimmelpenning syndrome.Lesions were partially removed by surgical excision.%患者女,17岁,右侧头面颈部泛发性褐色疣状增殖性皮损17年.右眼下睑泪囊区触及一质硬囊性肿物,右下泪小点缺如.眼眶核磁平扫:右眼泪囊区可见一类圆形肿物,压及筛窦及眼外肌.头面部皮损组织病理示:表皮角化过度,棘层增生肥厚,表皮乳头瘤样增生,真皮内可见大量成熟及不成熟的皮脂腺结构,部分见异位的大汗腺,诊断为皮脂腺痣.口腔右侧黏膜,包括右侧上腭及右半舌部见疣状赘生物;口腔全景片示右侧上下颌骨及牙槽骨发育欠佳.此外,双上肢可见大面积咖啡色斑片及褐色斑点.患者智力中等,既往有惊厥史.诊断:Schimmelpenning综合征.经皮肤外科手术后去除部分皮损.

  9. Endoscopic survey of esophageal cancer in a high-risk area of China

    Institute of Scientific and Technical Information of China (English)

    Xu-Jing Lu; Jun Hou; Zhi-Feng Chen; Cui-Lan Guo; Shao-Sen Li; Wen-Long Bai; Guo-Liang Jin; Yu-Xia Wang; Fan-Shu Meng; Feng Gao

    2004-01-01

    AIM: To characterize the histological types of esophageal and cardiac mucosa by endoscopic survey of a population in a high-risk area of esophageal cancer of China.METHODS: A selected cohort of residents in Cixian County during December 2001 and May 2002 was surveyed by using Lugol′s staining, followed by computer-based statistical analysis of the data with SPSS 10.0 software.RESULTS: Histologically, the detection rates of squamous epithelial acanthosis, squamous epithelial atrophy, and basal cell hyperplasia in the esophagus were 1.9% (38/2 013),0.1% (3/2 013) and 0.9% (18/2 013) respectively, and those of mild, moderate, and severe esophagitis were 34.9% (703/2 013), 1.6% (33/2 013) and 0.2% (2/2 013)respectively. Mild, moderate, and severe esophageal dysplasia were detected in 8.6% (172/2 013), 7.8% (157/2 013) and 2.6% (53/2 013) respectively in the selected population,whereas in situ carcinoma, intramucosal carcinoma, invasive squamous carcinoma of the esophagus in 2.5% (50/2 013),0.2% (4/2 013) and 0.7% (14/2 013) respectively. The detection rates of non-atrophic gastritis and atrophic gastritis of the cardia were 36.3% (730/2 013) and 11.5% (232/2 013) respectively,with mild and severe dysplasia of the cardia detected in 2.5% (51/2 013) and 0.8% (17/2 013), respectively, in this population; the rates of intramucosal adenocarcinoma and invasive adenocarcinoma of the cardia were 0.1% (3/2 013)and 0.8% (17/2 013) respectively. The detection rate of esophageal cancer at early stage was 79.4% (54/68). The survey rate (ratio of examined population to expected population) was 73.8% (2 013/2 725).CONCLUSION: Histologic types of the esophageal and cardiac mucosa were characterized by endoscopic survey in a high-risk population of esophageal cancer, which may help the early detection and treatment of esophageal and cardiac cancers and dysplasia, and reduce the mortality of such malignancies.

  10. Analysis of 24 Cases of Becker's Nevus%Becker痣24例分析

    Institute of Scientific and Technical Information of China (English)

    李钟洙; 黄一锦; 张召力; 廖永强; 张海萍

    2012-01-01

    目的 探讨Becker痣的临床特点、合并症和组织病理表现.方法 回顾性分析24例Becker痣临床和病理资料.结果 24例均为散发病例,发病年龄主要集中在10 ~ 20岁(83.33%),发病部位以躯干前部和肩胛部居多(45.83%),典型皮损为较大的单侧分布的多毛的色素沉着斑,组织病理主要表现是表皮不同程度角化过度和棘层肥厚,表皮突较规则地向下延伸,基底层黑素颗粒增多,真皮网状层常见较大的形态不规则的平滑肌纤维束,伴有并发症共5例(20.83%).结论 根据Becker痣典型皮损特点结合组织病理表现临床可以确诊,但是做为一种遗传相关的错构瘤可伴发其他皮肤肌肉骨骼异常,需仔细探明.%Objective To survey the Clinical features, complications and pathological manifestations of Becker's nevus. Methods The clinical and pathological materials of 24 cases of Becker' s nevus were analysed retrospectively. Results All of 24 cases occurred sporadically. The age of onset was mainly around 10 — 20 years old (83. 33% ). The lesions were located predominantly on the anterior trunk or on the scapular region (45.83% ). The typical primary lesion was large, unilateral hyperpigmented patch with hypertrichosis. The histopathology showed varying degrees of hyperkeratosis and acanthosis of the epidermis, regular elongation of the rete ridges,and basal layer hyperpigmentation ,usually larger sized and irregularly formed smooth muscle fiber scattered haphazardly in reticular dermis. Five cases were associated with different kinds of complication (20. 83% ). Conclusion Becker's nevus can be confirmed on the basis of clinical features and pathological examination. As a genetic-related hamartoma, however, Becker' s nevus may be accompanied with other cutaneous and musculoskeletal anomalies that need to be carefully proven.

  11. Effect of Probiotic Consortium on the Local Inflammatory Process in Chronic Periodontitis

    Directory of Open Access Journals (Sweden)

    Zhanagul Khasenbekova

    2014-01-01

    with a merger of the acanthosis outgrowths and a formation of the fibrotic nodules. Biochemical investigations did not show significant changes in the indicators. Conclusions: In the settings of the chronic periodontitis model, the use of gelatin-based probiotic consortium consisting of Lactobacillus casei subsp. pseudoplantarum, Lactobacillus caseisubsp.casei, L.fermentum, L. helveticus. at 2.5 x 109 CFU/ml viable cell numbers lead to the reduction of the local inflammatory manifestations of the periodontitis in 14 days of treatment. 

  12. 丘疹性红皮病%Papuloerythroderma

    Institute of Scientific and Technical Information of China (English)

    周乃慧; 陈爱明

    2012-01-01

    A 58-year-old woman has a 2-year history of pruritic eruption. Clinically, flat topped, red-to-brown and coalescing papules were presented on the back, buttocks and the extensor aspect of the upper limbs. The lesions spared the axillary, inguinal regions and cubital fossae and skin folds on the abdomen and sub-mammary area, resulting in the typical deck-chair' sign. The back showed a papular eruption following the Langer's line. The abnormal laboratory findings were lymphopenia and an elevated serum IgE level. The skin histopathology evidenced the hyperkeratosis and acanthosis in the epidermis, and a small number lymphocyte infiltration around the blood vessel in the upper dermis. Immunohistochemisty showed the positive staining of CD3, CD45RO, CD79a, KP-1, weakly positive staining of CD20, and negative staining of granzyme B and CD56. The diagnosis of papuloerythroderma is made.%报告1例丘疹性红皮病.患者女,58岁.因躯干、双上肢伸侧及臀部红褐色丘疹伴瘙痒2年就诊.皮肤科检查:躯干、双上肢伸侧及臀部可见较多红褐色的扁平丘疹,表面少许鳞屑,有融合趋向,呈红皮病样损害.实验室检查:外周血淋巴细胞减少,血清IgE增高.皮损组织病理学检查:角化过度,棘层肥厚,表皮突细长下延,真皮浅层血管周围少量淋巴细胞浸润.皮损免疫组化:CD3、CD45RO、CD79a及KP-1阳性,CD20弱阳性,granzyme B及CD56阴性.诊断:丘疹性红皮病.

  13. Effects of in Utero Exposure of C57BL/6J Mice to 2,3,7,8-Tetrachlorodibenzo-p-dioxin on Epidermal Permeability Barrier Development and Function

    Science.gov (United States)

    Muenyi, Clarisse S.; Carrion, Sandra Leon; Jones, Lynn A.; Kennedy, Lawrence H.; Slominski, Andrzej T.

    2014-01-01

    Background: Development of the epidermal permeability barrier (EPB) is essential for neonatal life. Defects in this barrier are found in many skin diseases such as atopic dermatitis. Objective: We investigated the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the development and function of the EPB. Methods: Timed-pregnant C57BL/6J mice were gavaged with corn oil or TCDD (10 μg/kg body weight) on gestation day 12. Embryos were harvested on embryonic day (E) 15, E16, E17, and postnatal day (PND) 1. Results: A skin permeability assay showed that TCDD accelerated the development of the EPB, beginning at E15. This was accompanied by a significant decrease in transepidermal water loss (TEWL), enhanced stratification, and formation of the stratum corneum (SC). The levels of several ceramides were significantly increased at E15 and E16. PND1 histology revealed TCDD-induced acanthosis and epidermal hyperkeratosis. This was accompanied by disrupted epidermal tight junction (TJ) function, with increased dye leakage at the terminal claudin-1–staining TJs of the stratum granulosum. Because the animals did not have enhanced rates of TEWL, a commonly observed phenotype in animals with TJ defects, we performed tape-stripping. Removal of most of the SC resulted in a significant increase in TEWL in TCDD-exposed PND1 pups compared with their control group. Conclusions: These findings demonstrate that in utero exposure to TCDD accelerates the formation of an abnormal EPB with leaky TJs, warranting further study of environmental exposures, epithelial TJ integrity, and atopic disease. Citation: Muenyi CS, Leon Carrion S, Jones LA, Kennedy LH, Slominski AT, Sutter CH, Sutter TR. 2014. Effects of in utero exposure of C57BL/6J mice to 2,3,7,8-tetrachlorodibenzo-p-dioxin on epidermal permeability barrier development and function. Environ Health Perspect 122:1052–1058; http://dx.doi.org/10.1289/ehp.1308045 PMID:24904982

  14. Outbreaks of vesicular disease caused by Vaccinia virus in dairy cattle from Goiás State, Brazil (2010-2012

    Directory of Open Access Journals (Sweden)

    Fabiano J.F. de Sant'Ana

    2013-07-01

    Full Text Available Cases of vesicular and exanthematic disease by Vaccinia virus (VACV have been reported in dairy herds of several Brazilian regions, occasionally also affecting humans. The present article describes eight outbreaks of vesicular disease caused by VACV in dairy herds of six counties of Goiás state, Midwestern Brazil (2010-2012, involving a total of 122 cows, 12 calves and 11 people. Dairy cows (3 to 9 years old were affected in all cases and calves (2 to 9 months old were affected in five outbreaks, presenting oral lesions. The morbidity ranged between 8 and 100% in cows, and 1.5 to 31% in calves. In the cows, the clinical signs started with vesicles (2-7mm, painful and coalescent papules (3-8 mm, which resulted in ulcers (5-25mm and scabs in teats, and, occasionally, in the muzzle. The clinical course lasted from 16 to 26 days. The histopathology of bovine skin samples revealed superficial perivascular inflammatory infiltrate of lymphocytes, plasma cells, neutrophils, macrophages and multifocal areas of acanthosis, spongiosis, hipergranulosis and parakeratotic or orthokeratotic hyperkeratosis with adjacent focally extensive ulcers. Eosinophilic inclusion bodies were noted in the cytoplasm of the keratinocytes. PCR to vgf gene of Orthopoxvirus was positive in samples collected from all outbreaks, and in some cases, genomic VACV sequences were identified by nucleotide sequencing of the PCR amplicons. Infectious virus was isolated in cell culture from scabs from one outbreak. Antibodies to Orthopoxvirus were detected in at least 3 or 4 animals in most outbreaks, by ELISA (outbreaks 1, 2, 3, 4, 5 and 7 or virus-neutralization (outbreak 6. Neutralizing titers ranging from 8 to 64 in outbreak 6. In all outbreaks, VACV infection was suspected based on the clinical and pathological findings and it was confirmed by laboratory tests. Upon the etiological confirmation, other agents associated with vesicular disease were discarded. In all outbreaks, at least

  15. Hiperplasia epitelial focal (doença de Heck em descendente de índios brasileiros: relato de caso Focal epithelial hyperplasia (Heck's disease in Brazilian indian descent: report of a case

    Directory of Open Access Journals (Sweden)

    Pedro Paulo de Andrade Santos

    2007-12-01

    Full Text Available A hiperplasia epitelial focal, ou doença de Heck, é uma enfermidade rara, benigna, que afeta a mucosa oral de crianças e adultos jovens de diversas regiões do mundo e em diferentes grupos étnicos, como indígenas e esquimós. Apresenta correlação com o papilomavírus humano (HPV no qual os tipos 13 e 32 têm sido consistentemente detectados nessas lesões. Este artigo relata um caso de uma paciente de 18 anos de idade, descendente de índios potiguares, que compareceu ao serviço de estomatologia da Universidade Federal do Rio Grande do Norte (UFRN, exibindo lesões bem definidas, arredondadas, planas, localizadas em cavidade oral, com tempo de evolução de aproximadamente dois anos. As lesões foram submetidas a biópsias incisionais, constatado-se no exame histopatológico alterações epiteliais, como acantose, cristas epiteliais em forma de "taco de golfe" além de células mitosóides. Esses achados histopatológicos foram compatíveis com a hipótese clínica de hiperplasia epitelial focal (doença de Heck.The focal epithelial hyperplasia or Heck's disease is a benign rare pathology, that affects children and young adults oral mucosal in many world regions, and different ethnic groups, for example Indians and Eskimos. Presents correlation with the subtypes 13 and 32 of human papillomavirus (HPV. This article report a case of an 18-year-old patient, descent of potiguar indian, attended in stomatology service of Federal University of Rio Grande do Norte (UFRN, presenting well defined lesions, round, plane, localized in oral cavity with an evolution of two years. The lesions were submitted to incisional biopsies, verifying in histopathologic exam, epithelial alterations, like acanthosis, epithelial projections in "parquet block of golf" beyond mitosoid cells. These histopathological findings were compatible with clinical hypothesis of focal epithelial hyperplasia (Heck's disease.

  16. 扁平苔藓样药疹1例%A Case of Lichenoid Drug Eruption

    Institute of Scientific and Technical Information of China (English)

    程燕; 陈晓玫; 汪盛

    2011-01-01

    A 77-year-old man was admitted in the hospital with 7 days history of itching crythema and papules all over the body .Two months before onset of skin lesions he had taken irbesartan,metoprolol,amlodipine besylate,atorvastain calcium tablets,aspirin,clopidogrel bisulfate and esomeprazle manesiumenteric-coated tablets for cardiovascular disease.Severn days ago ,the fava bean like erythema and papules scattered appeared or the whole body,expecially on the proximal end of limbs and the trunk with intense itching .Previously,no drug allergy happened to him.Histological examination showed hyperkeratosis,acanthosis,liquefaction of basal cells and a band-like lymphocytes infiltrate in the dermis.Cytoid bodies and cosinophils were scattered in the superficial dermis .According to the medical history ,clinical manifestations and histological presentation,a diagnosis of lichenoid drug eruption was established.%患者男,77岁.全身出现红斑和丘疹,伴瘙痒7天.2个月前,患者口服"厄贝沙坦、美托洛尔、苯磺酸氨氯地平片、阿托伐他汀钙、阿司匹林、硫酸氯吡格雷、埃索美拉唑镁肠溶片"等药物.7天前,全身出现散在分布的粟粒至蚕豆大红斑和丘疹,以躯干和四肢近心端为著,瘙痒剧烈.既往无药物过敏史.左小腿胫前新鲜水疱组织病理示:表皮角化过度,棘层肥厚,基底细胞空泡样变性,真皮浅层以淋巴细胞为主的带状浸润,可见嗜酸性粒细胞及胶样小体.诊断:扁平苔藓样药疹.

  17. Predicting effects of environmental change on a migratory herbivore

    Science.gov (United States)

    Stillman, R A; Wood, K A; Gilkerson, Whelan; Elkinton, E; Black, J. M.; Ward, David H.; Petrie, M.

    2015-01-01

    Changes in climate, food abundance and disturbance from humans threaten the ability of species to successfully use stopover sites and migrate between non-breeding and breeding areas. To devise successful conservation strategies for migratory species we need to be able to predict how such changes will affect both individuals and populations. Such predictions should ideally be process-based, focusing on the mechanisms through which changes alter individual physiological state and behavior. In this study we use a process-based model to evaluate how Black Brant (Branta bernicla nigricans) foraging on common eelgrass (Zostera marina) at a stopover site (Humboldt Bay, USA), may be affected by changes in sea level, food abundance and disturbance. The model is individual-based, with empirically based parameters, and incorporates the immigration of birds into the site, tidal changes in availability of eelgrass, seasonal and depth-related changes in eelgrass biomass, foraging behavior and energetics of the birds, and their mass-dependent decisions to emigrate. The model is validated by comparing predictions to observations across a range of system properties including the time birds spent foraging, probability of birds emigrating, mean stopover duration, peak bird numbers, rates of mass gain and distribution of birds within the site: all 11 predictions were within 35% of the observed value, and 8 within 20%. The model predicted that the eelgrass within the site could potentially support up to five times as many birds as currently use the site. Future predictions indicated that the rate of mass gain and mean stopover duration were relatively insensitive to sea level rise over the next 100 years, primarily because eelgrass habitat could redistribute shoreward into intertidal mudflats within the site to compensate for higher sea levels. In contrast, the rate of mass gain and mean stopover duration were sensitive to changes in total eelgrass biomass and the percentage of time

  18. Síndrome metabólico: definición, historia, criterios

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Pineda

    2008-03-01

    Full Text Available El síndrome metabólico es un conjunto de factores de riesgo para diabetes mellitus tipo 2 (DM2 y enfermedad cardiovascular, caracterizado por la presencia de resistencia a la insulina e hiperinsulinismo compensador asociados con trastornos del metabolismo de los carbohidratos y lípidos, cifras elevadas de presión arterial, y obesidad. Se hizo una revisión en Medline que arrojó 6,366 entradas para síndrome metabólico. Desde principios del siglo XX se describieron asociaciones de factores de riesgo. En 1988, Reaven observó que varios factores de riesgo (dislipidemia, hipertensión, hiperglicemia tendían a estar juntos. Este conjunto lo llamó síndrome X. El término síndrome metabólico como entidad diagnóstica con criterios definidos fue introducida por la OMS en 1998. La prevalencia del síndrome metabólico varía según factores como género, edad, etnia, pero se ubica entre 15% y 40%; es mayor en la población de origen hispano. Existen varios criterios para el diagnóstico de síndrome metabólico. El más conocido es del ATP III, donde se deben cumplir 3 ó más de los siguientes: perímetro abdominal elevado (>102 cm en hombres y >88 cm en mujeres, TG >150 mg/dl, HDL bajo (hombres 130/85 mm Hg, glicemia >110 mg/dl incluyendo diabetes mellitus. Se consideran otros factores como trombogénesis, inflamación, ácido úrico, estrés, cigarrillo, sedentarismo, edad, origen étnico, acantosis nigricans, síndrome de ovario poliquístico, microalbuminuria, hipotiroidismo primario, uso de inhibidores de proteasa para pacientes con VIH, exceso de glucocorticoides endógeno o exógeno.

  19. Mercury bioaccumulation and biomagnification in Ozark stream ecosystems

    Science.gov (United States)

    Schmitt, Christopher J.; Stricker, Craig A.; Brumbaugh, William G.

    2011-01-01

    Crayfish (Orconectes spp.), Asian clam (Corbicula fluminea), northern hog sucker (hog sucker; Hypentelium nigricans), and smallmouth bass (smallmouth; Micropterus dolomieu) from streams in southeastern Missouri (USA) were analyzed for total mercury (HgT) and for stable isotopes of carbon (δ13C), nitrogen (δ15N), and sulfur (δ34S) to discern Hg transfer pathways. HgT concentrations were generally lowest in crayfish (0.005-0.112 μg/g dw) and highest in smallmouth (0.093-4.041 μg/g dw), as was δ15N. HgT was also lower and δ15N was higher in all biota from a stream draining a more heavily populated historical lead-zinc mining area than from similar sites with mostly undeveloped forested watersheds. δ13C in biota was lowest at spring-influenced sites, reflecting CO2 inputs and temperature influences, and δ34S increased from south to north in all taxa. However, HgT was not strongly correlated with either δ13C or δ34S in biota. Trophic position (TP) computed from crayfish δ15N was lower in hog suckers (mean=2.8) than in smallmouth (mean=3.2), but not at all sites. HgT, δ13C, δ34S, and TP in hog suckers increased with total length (length) at some sites, indicating site-specific ontogenetic diet shifts. Changes with length were less evident in smallmouth. Length-adjusted HgT site means in both species were strongly correlated with HgT in crayfish (r2=0.97, Pr2=0.02, P>0.05). NCOVA and regression models incorporating only TP and, for hog suckers, length, accurately and precisely predicted HgT concentrations in both fish species from all locations. Although low compared to many areas of the USA, HgT (and therefore methylmercury) concentrations in smallmouth and hog suckers are sufficiently high to represent a threat to human health and wildlife. Our data indicate that in Ozark streams, Hg concentrations in crayfish are at least partly determined by their diet, with concentrations in hog suckers, smallmouth, and possibly other higher-level consumers largely

  20. Síndrome metabólico: definición, historia, criterios

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Pineda

    2009-11-01

    Full Text Available El síndrome metabólico es un conjunto de factores de riesgo para diabetes mellitus tipo 2 (DM2 y enfermedad cardiovascular, caracterizado por la presencia de resistencia a la insulina e hiperinsulinismo compensador asociados con trastornos del metabolismo de los carbohidratos y lípidos, cifras elevadas de presión arterial, y obesidad. Se hizo una revisión en Medline que arrojó 6,366 entradas para síndrome metabólico. Desde principios del siglo XX se describieron asociaciones de factores de riesgo. En 1988, Reaven observó que varios factores de riesgo (dislipidemia, hipertensión, hiperglicemia tendían a estar juntos. Este conjunto lo llamó síndrome X. El término síndrome metabólico como entidad diagnóstica con criterios definidos fue introducida por la OMS en 1998. La prevalencia del síndrome metabólico varía según factores como género, edad, etnia, pero se ubica entre 15% y 40%; es mayor en la población de origen hispano. Existen varios criterios para el diagnóstico de síndrome metabólico. El más conocido es del ATP III, donde se deben cumplir 3 ó más de los siguientes: perímetro abdominal elevado (>102 cm en hombres y >88 cm en mujeres, TG >150 mg/dl, HDL bajo (hombres 130/85 mm Hg, glicemia >110 mg/dl incluyendo diabetes mellitus. Se consideran otros factores como trombogénesis, inflamación, ácido úrico, estrés, cigarrillo, sedentarismo, edad, origen étnico, acantosis nigricans, síndrome de ovario poliquístico, microalbuminuria, hipotiroidismo primario, uso de inhibidores de proteasa para pacientes con VIH, exceso de glucocorticoides endógeno o exógeno.

  1. Preparation of ZnO/TiO2 heterostructure and its antibacterial activity%ZnO/TiO2异质复合材料的制备及抗菌性能研究

    Institute of Scientific and Technical Information of China (English)

    洪杰; 左施旖旎; 王光辉; 朱长俊; 李雷; 汪海东

    2016-01-01

    以钛酸四正丁酯、氯化锌、冰醋酸等为原料,采用水热法制备了ZnO/TiO2复合光催化剂。通过X-射线衍射仪( XRD)、扫描电子显微镜( SEM)、能谱仪( EDS)对所制备纳米复合材料的物相组成以及微观形貌进行了表征。采用牛津杯法以抑菌圈大小为评价指标,研究了不同工艺条件下制得的ZnO/TiO2复合材料对大肠杆菌、金黄色葡萄球菌、根霉、黑曲霉抗菌性能的影响。研究结果表明,重量比为1:10的ZnO/TiO2复合材料具有良好的抗菌性能。%The composite photocatalyst of ZnO/TiO2 were synthesized by the hydrothermal method using tetrabutyltitanate,zinc chlo-ride and aceticacid as raw materials. The effects of reactant concentration on the morphologyand structural were investigated by X-Ray Diffraction(XRD),Scanning Electron Microscope(SEM),Energy Dispersive Spectrometer(EDS). With the antibacterial circle size as the evaluation pointer by Oxford Cup method,the antibacterial properties of ZnO/TiO2 nanocomposite powders on Escherichi-a coli,Staphyloccocus aureus, Rhizopus nigricans and Aspergullus niger were studied which prepared in different process condi-tions,respectively. The results showed that the antibacterial properties of the ZnO/TiO2 nanocomposite powders was better than the others when the weight ratio of ZnO to TiO2 was 1∶10.

  2. Determinación del potencial promotor del crecimiento vegetal de Azotobacter spp. aislados de la rizósfera de malezas en cultivos de maíz (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Luis H. León

    2015-01-01

    Full Text Available Los fertilizantes químicos representan entre 20% y 30% de los costos de producción de un cultivo, utilizados correctamente incrementan la productividad y rentabilidad; sin embargo, cada año aumenta la cantidad de fertilizantes por aplicar, debido a la deficiencia de adsorción en el suelo y absorción por la planta. Siendo el maíz el tercer cultivo de importancia en Perú, con un impacto significativo en la actividad económica y social, en el 2014, solo el 40% del maíz ofertado correspondió a la producción nacional. En busca de alternativas para disminuir el uso de fertilizantes químicos se realizan investigaciones con denominadas rizobacterias promotoras del crecimiento vegetal (PGPR, por sus siglas en ingles. Se identificaron 37 malezas en cultivos de maíz procedentes de campos agrícolas de los distritos de Monsefú y Reque, Región Lambayeque, siendo dicotiledóneas predominantes con 68 % con respecto a monocotiledóneas con 32%. Las bacterias se aislaron de la rizósfera de malezas, obteniendo 305 cultivos puros de bacterias, de los cuales 133 cultivos puros (43,7% se identificaron como Azotobacter spp., investigándose su reacción bioquímica en reducción de nitratos, utilización de sacarosa, glucosa, maltosa, manitol, ramnosa, glicerol y sorbitol, identificándose A. vinelandii (58%, A. paspali (13%, A. armeniacus (8%, A. nigricans (8% y en 13 cultivos no se identificó la especie. Con los cultivos Azotobacter spp. se cuantificó hasta 36,03 ppm de nitrógeno fijado como amonio; 60,75 ppm de ácido indol acético y 6,06 ppm de fósforo solubilizado, se determinó actividad antagónica contra Fusarium verticillioides, proteolítica y quitinolítica y. El 20% de Azotobacter spp. no afectó la emergencia de maíz amarillo duro hibrido simple AGRI- 144, el 33 % la afectó positivamente y el 47% la afectó negativamente. A su vez, ningún Azotobacter spp. afectó la sobrevivencia. Demostrándose el potencial promotor del

  3. Study on Antimicrobial Activity of Extract from Opuntia Dillenii against Common Food Pollutant Bacteria%仙人掌提取物对常见食品污染菌的抑菌特性研究

    Institute of Scientific and Technical Information of China (English)

    豁银强; 郑建荣; 汤尚文; 吴进菊

    2012-01-01

    用水和95%乙醇2种溶剂提取仙人掌的有效成分,测试了2种提取物的抑菌圈直径,对乙醇提取物的最小抑菌浓度(MIC)进行了测定,研究了温度、pH、盐、糖对抑菌活性的影响。结果表明,仙人掌95%乙醇浸提物的抑菌效果优于水浸提物;仙人掌乙醇浸提液的最小抑菌浓度分别为:大肠杆菌3.125 mg/mL,沙门氏菌、金黄色葡萄球菌、枯草芽抱杆菌、黑根霉6.25 mg/mL,桔青霉12.5 mg/mL,毕赤酵母、啤酒酵母25 mg/mL,黑曲霉50 mg/mL;提取物热稳定性好,在pH4~6的范围内具有稳定的抑菌作用,提取物与盐、糖具有良好的协同抑菌作用。%Effective components of opuntia were extracted by water and ethanol as solvent,antibacterial zone diameters of extract were tested,the effects of temperature,pH,salt,sugar on inhibition activity were studied.Results indicate that antibacterial effect of 95% ethanol extract was better,the minimum inhibition concentration(MIC) of ethanol extract was as follows: 3.125 mg/mL against Escherichia coli,6.25 mg/mL against Salmonella,Staphylococcus aureus,Bacillus subtili,and Rhizopus nigricans,12.5 mg/mL against Penicillium citrinum,25 mg/mL against Saccharomyces cerevisiae and Pichia pastoris,50 mg/mL against Aspergillus niger.It was also proved that the extract has better thermal stability and it has stable antibacterial effect in pH4-6.Besides,the addition of sucrose or NaCl can improve the antimicrobial activity.

  4. Eight new species of Macvicaria Gibson and Bray, 1982 (Digenea: Opecoelidae from temperate marine fishes of Australia

    Directory of Open Access Journals (Sweden)

    Thelma Aken’Ova

    2008-07-01

    Full Text Available Nine morphologically distinct species of Macvicaria Gibson & Bray, 1982 were recovered from six families, ten genera and twelve species of marine fishes in the waters off the coast of Queensland, Western Australia and South Australia: M. shotteri n. sp. in Apogon fasciatus (type-host, Sillaginodes punctatus and Sillago bassensis from Moreton Bay (type-locality, off southern Western Australia and off Kangaroo Island, South Australia; M. mekistomorphe n. sp. in Sillago maculata from Moreton Bay; M. mutovitellina n. sp. in Dactylophora nigricans from off southern Western Australia; M. flexuomeatus n. sp. in Goniistius gibbosus (type-host and Cheilodactylus rubrolabiatus from southern Western Australia; M. vitellocopiosa n. sp. in Sillaginodes punctatus from off Kangaroo Island, South Australia; M. dextrocaula n. sp. in Notolabrus parilus (type-host and N. fucicola (Richardson (Labridae from off southern Western Australia (type-locality and Kangaroo Island, South Australia; M. heronensis Bray & Cribb, 1989 in Trachinotus coppingeri off Stradbroke Island, Queensland; M. adomeae n. sp. in Sillaginodes punctatus off Kangaroo Island, South Australia; M. kingscotensis n. sp. in Neoodax balteatus (type-host and Haletta semifasciata off Kangaroo Island, South Australia. We have used the following characters to distinguish between the species of Macvicaria: general body form; length/width ratio; size of cirrussac; length of forebody; post-testicular extent of caeca; egg size; and the position of the genital pore. Most of the species of Macvicaria showed interspecific differences in all of the characters enumerated, the most dramatic being in the position of the genital pore in one species. All 11 specimens recovered from 2 species of Notolabrus were found to have a dextral genital pore. This is the second report of amphitypy in the Opecoelidae. All of the host species, apart from Apogon fasciatus, are endemic to Australian or Australian and New Zealand

  5. The role of surface water redistribution in an area of patterned vegetation in a semi-arid environment, south-west Niger

    Science.gov (United States)

    Bromley, J.; Brouwer, J.; Barker, A. P.; Gaze, S. R.; Valentine, C.

    1997-11-01

    The surface hydrology of a semi-arid area of patterned vegetation in south-west Niger is described. In this region alternating bands of vegetation and bare ground aligned along the contours of a gently sloping terrain give rise to a phenomenon known as `brousse tigrée' (tiger bush). At the selected study site the vegetation bands are 10-30 m wide, separated by 50-100-m-wide bands of bare ground. Five species of shrub dominate, Guiera senegalensis, Combretum micranthum, C. nigricans, Acacia ataxacantha and A. macrostachya. Herbaceous vegetation is generally limited to the upslope edges of vegetation bands. A comprehensive field programme was undertaken to investigate the hydrology. Topographic, vegetation and surface feature surveys were carried out in conjunction with the measurement of rainfall, surface and subsurface hydraulic conductivity, particle size and soil moisture content. Four types of vegetation class are recognised, each tending to occupy a constant position relative to the others and to the regional slope. In a downslope direction the classes are: bare ground, grassy open bush, closed bush, bare open bush, bare ground etc. The nature of the ground surface is closely linked to the vegetation class. Over the bare, bare open and grassy open classes various types of surface crust are present with each type of crust tending to occupy a constant position on the regional slope relative to the vegetation class and other crust types. Below closed bush crusts are generally absent. The typical downslope sequence from the downslope boundary of a vegetation band is: structural (sieving) crust→erosion crust→(gravel crust)→sedimentation crust→microphytic sedimentation crust→no crust→sieving crust, etc. It is also shown that these crust types are dynamic and evolve from one to the other as hydrological conditions change. Hydraulic conductivities of surface crusts are low, typically falling within the range 10 -6-10 -7 m s -1. The presence of large

  6. 木耳emg1基因克隆与生物信息学分析%Cloning and Bioinformatics Analysis of emg1 Gene of Auricularia auricular-judae

    Institute of Scientific and Technical Information of China (English)

    方明; 姚方杰; 王晓娥; 陈影

    2013-01-01

    PCR products Aa-2 were amplified with degenerate primers designed according to the cDNA sequences of Auricularia nigricans and Auricularia delicata with the length of 1 075 bp.The E value of Aa-2 is 0 by blastn with A.delicata genome sequence of JGI databank and 1.82e-112 by blastx with Auricularia delicata emg 1 gene of JGI databank.775 bp are identical between the Aa-2 sequence and the Auricularia auricularia-judae emg1 gene,among which the genome was sequenced by our lab.The homology of part Aa-2 fragments to emg1 cDNA fragments of Sporisorium reilianum is 91% by blastn.The E value of Aa-2 fragments is 2.66e-43 compared with the emg1 superfamily protein sequence.The Aa-2 fragment was preliminarily identified as emg1 CDS.%以毛木耳和皱木耳的cDNA序列为模板设计简并引物并进行扩增,获得1条长度为1075 bp的序列Aa-2;与JGI数据库中皱木耳基因组Blastn比对E值为0;与编码emg1的氨基酸序列Blastx同源性比对的E值高达1.82e-112.与木耳全基因组序列中注释为emg1的核酸序列有775 bp的序列完全一致.经GenBank数据库Blastn同源比对,与玉米丝黑穗菌emg1蛋白cDNA的部分序列相似性达91%.经GenBank中Conserved domains搜索,Aa-2序列与emg1蛋白超家族序列同源比对的E值达2.66e-43,初步判定Aa-2的部分序列为emg1蛋白的编码序列.

  7. Low total mercury in Caiman yacare (Alligatoridae) as compared to carnivorous, and non-carnivorous fish consumed by Amazonian indigenous communities.

    Science.gov (United States)

    Rivera, S J; Pacheco, L F; Achá, D; Molina, C I; Miranda-Chumacero, G

    2016-11-01

    Mercury contamination in the River Beni basin is an important health risk factor, primarily for indigenous communities that live along the river. Among them are the Tacana, living in their original territory with sustainable use of their natural resources, consuming fish, Caiman yacare, and other riverine resources as their main source of protein. To assess mercury exposure to Tacana people, total mercury (THg) was evaluated in the muscle of seven commercial fish, and Caiman yacare (yacare caiman) during 2007 and 2008. THg was extracted by acid digestion and concentrations were determined by atomic absorption spectrometry. Mean mercury concentrations in C. yacare was 0.21 ± 0.22 μg g(-1)Hg w.w. (wet weight), which is lower than expected given its high trophic level, and its long life-span. It is possible that mercury in C. yacare is accumulated in other organs, not included in this study; but it is also possible that physiological mechanisms are involved that help caimans get rid of ingested mercury, or simply that C. yacare's diverse diet reduces THg accumulation. Carnivorous fishes (Pygocentrus nattereri, Pseudoplatystoma tigrinum, Zungaro zungaro, Plagioscion squamosissimus, and Leiarius marmoratus) had the highest total mercury concentrations, ranging from 0.35 to 1.27 μg g(-1)Hg w.w. moreover, most were above the limit recommended by WHO (0.5 μg g(-1)Hg w.w.); except for Leiarius marmuratus, which presented a mean of 0.353 ± 0.322 μg g(-1)Hg w.w. The two non-carnivorous fish species (Prochilodus nigricans, and Piaractus brachypomus) present mean concentrations of 0.099 ± 0.027, and 0.041 ± 0.019 μg g(-1)Hg w.w., respectively. Finally, recommendations on the consumption habits of Tacana communities are discussed.

  8. Análise histopatológica comparativa entre líquen plano oral e cutâneo Comparative histopathological analysis between oral and cutaneous lichen planus

    Directory of Open Access Journals (Sweden)

    Silvia Elena Navas-Alfaro

    2003-01-01

    ência rara no LPO. Acantose em dente de serra é achado pouco freqüente no LPO, porém vacuolização basal está sempre presente associada a infiltrado linfocitário em faixa; plasmócitos podem acompanhar linfócitos no LPO, mas estão ausentes do LPC.BACKGROUND: Lichen planus is a relatively common chronic inflammatory disease affecting skin and mucous membranes, separately or together. It frequently involves the oral cavity, with or without concomitant cutaneous compromise. Microscopically, while skin presents well characteristic aspects, oral mucosa can present variations related to local characteristics of the mucosa in the oral cavity. OBJECTIVES: To compare histopathological alterations of oral lichen planus and cutaneous lichen planus with the study of the inflammatory infiltrate components. METHODS: 59 biopsies (29 of OLP and 30 of CLP, formalin-fixed, paraffin-embedded were studied. Semi-quantitative analysis of both epidermal and dermal alterations was performed using the hematoxylin-eosin and periodic acid-Schiff stains. RESULTS: For CLP the classic aspects of orthokeratosis, wedge shaped hypergranulosis and saw tooth rete ridges were observed. Such aspects differ from those of OLP in which orthokeratosis and thickened granular layer happen less frequently; instead, acquisition of those layers is observed in places where they are usually absent. Basal cell vacuolar alteration and effacement of the epithelium-conjunctive limit prevailed in both locations (skin and oral mucosa and lymphocytes and histiocytes were the inflammatory cells present in all cases. CONCLUSIONS: Classic alterations as compact orthokeratosis, wedge shaped hypergranulosis, saw tooth rete ridges acanthosis and basal cell vacuolar alteration associates to lymphocytes in superficial band-like pattern are diagnostic of CLP. However, for OLP, the particular own characteristics of oral mucosa in the biopsied place should be considered; also, subtler alterations of horny (light orthokeratosis and

  9. 中西太平洋金枪鱼延绳钓主要渔获物垂直结构的初步研究%Vertical distribution of main species captured by tuna longline fishery in the Western and Central Pacific

    Institute of Scientific and Technical Information of China (English)

    栾松鹤; 戴小杰; 田思泉; 张艳波

    2015-01-01

    line,length of branch line,main line length between branch lines and so on.The data of fishing gear were used to calculate caught depth by catenary equation.The actual average depth of each hook was measured based on the depth data recoreded by TDR.Based on depth data of longline hook recorded by TDR and capture hook position of pelagic species,shoaling rate of longline hook and vertical distribution were analyzed.The results were as follows:the absolute shoaling rate range of longline hook was 0.49 -44.73 m,and the average absolute shoaling rate was 23.29 m.The relative shoaling rate rang of longline hook was 0.65%-16.69%, and the average relative shoaling rate was 8.87%.The depth ranges of 12 main species were different.The species with the deepest depth was blue marlin (Makaira nigricans),and the species with the shallowest depth was Great barracuda (Lampris guttatus ).Cluster analysis showed that there were three vertical distribution structures among 12 main species;the first class with the shallowest depth was common dolphinfish (Coryphaena hippurus),great barracuda(Sphyraena barracuda),wahoo(Acanthocybium solandri);the second class with deeper depth was escolar (Lepidocybium flavobrunneum ),pelagic stingray (Dasyatis violacea ), albacore tuna (Thunnus alalunga ),yellowfin tuna (Thunnus albacares ),slender mola (Ranzania laevis ), skipjack tuna (Katsuwonus pelamis );the third class with the deepest depth was bigeye tuna (Thunnus abesus),longnose lancetfish(Alepisaurus ferox),blue marlin(Makaira nigricans);the mean depth and depth distributions of bycatch species were significantly different from that of the targeted albacore tuna.Those results show that the theoretical depth of the catenary equation has a certain reference value in the Western and Central Pacific.Deploying longline hooks at appropriate depths can greatly improve catch of target species,and reduce bycatch of protected species and untargeted species.

  10. Vírus da raiva em quirópteros naturalmente infectados no Estado de São Paulo, Brasil Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Karin Corrêa Scheffer

    2007-06-01

    Full Text Available OBJETIVO: Identificar as espécies de morcegos envolvidas na manutenção do ciclo da raiva, verificar a distribuição do vírus da raiva em tecidos e órgãos de morcegos e os períodos de mortalidade dos camundongos inoculados. MÉTODOS: A positividade para o vírus da raiva foi avaliada por imunofluorescência direta em morcegos de municípios do Estado de São Paulo, de abril de 2002 a novembro de 2003. A distribuição do vírus nos morcegos foi avaliada pela inoculação de camundongos e infecção de células N2A, com suspensões a 20% preparadas a partir de fragmentos de diversos órgãos e tecidos, além de cérebro e glândula salivar. A mortalidade dos camundongos foi observada diariamente, após inoculação intracerebral. RESULTADOS: Dos 4.393 morcegos pesquisados, 1,9% foram positivos para o vírus da raiva, pertencentes a dez gêneros, com predomínio de insetívoros. A média do período máximo de mortalidade dos camundongos pós-inoculação a partir de cérebros e glândulas salivares de morcegos hematófagos foi de 15,33±2,08 dias e 11,33±2,30 dias; insetívoros, 16,45±4,48 dias e 18,91±6,12 dias; e frugívoros, 12,60±2,13 dias e 15,67±4,82 dias, respectivamente. CONCLUSÕES: As espécies infectadas com o vírus da raiva foram: Artibeus lituratus, Artibeus sp., Myotis nigricans, Myotis sp., Eptesicus sp., Lasiurus ega, Lasiurus cinereus, Nyctinomops laticaudatus, Tadarida brasiliensis, Histiotus velatus, Molossus rufus, Eumops sp. e Desmodus rotundus. A pesquisa de vírus em diferentes tecidos e órgãos mostrou-se que os mais apropriados para o isolamento foram cérebro e glândulas salivares.OBJECTIVE: To identify the species of bats involved in maintaining the rabies cycle; to investigate the distribution of the rabies virus in the tissues and organs of bats and the time taken for mortality among inoculated mice. METHODS: From April 2002 to November 2003, bats from municipalities in the State of São Paulo were

  11. Combate ao Desmodus rotundus rotundus (E. Geoffroy,1810 na região cárstica de Cordisburgo e Curvelo, Minas Gerais Combat of Desmodus rotundus rotundus (E. Geofroy, 1810 in the Cordisburgo and Curvelo carstic region, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    E.O. Almeida

    2002-04-01

    Full Text Available Com o objetivo de avaliar em uma região cárstica o tipo de refúgio, as espécies de morcegos, a população de animais domésticos, as associações interespecíficas nas coabitações com outros mamíferos silvestres suscetíveis à raiva e a eficácia da warfarina aplicada no dorso do Desmodus rotundus rotundus foi realizada uma pesquisa de maio de 1998 a março de 2000, nos municípios de Cordisburgo e Curvelo, Minas Gerais. Em 49 refúgios vistoriados, 29 naturais e 20 artificiais, localizados em 14 propriedades, encontrou-se o Desmodus rotundus rotundus em 18 abrigos naturais. Destes, 17 eram cavernas formadas pela dissolução ou abatimento de rocha calcária, típica do carste, e um era túnel escavado na terra pela ação das águas de um rio. As características geomorfológicas e de localização espacial foram registradas com base nas coordenadas geográficas, obtidas com auxílio de um sensor geográfico de posição. Nesses abrigos foram capturados e identificados 1457 morcegos de 14 espécies, sendo 640 Glossophaga soricina (Pallas, 1766, 566 Desmodus rotundus (E. Geoffroy, 1810, 73 Anoura geoffroyi (Gray 1838, 58 Trachops cirrhosus (Spix, 1823, 38 Diphylla ecaudata ecaudata (Spix, 1823, 23 Platyhrrinus lineatus (E. Geoffroy, 1810, 16 Lasiurus ega (Gervais, 1856, 14 Carollia perspicillata (Linnaeus, 1758, 13 Phyllostomus hastatus hastatus (Pallas, 1767, 9 Artibeus lituratus (Olfers, 1818, 3 Mimmon bennettii (Gray, 1838, 2 Myotis nigricans (Schinz, 1821, 1 Eptesicus brasiliensis (Desmarest, 1819 e 1 Pygoderma bilabiatum (Wagner, 1843. Não se conseguiu isolar ou detectar o vírus rábico no cérebro de 25 hematófagos selecionados e em 52 de outras espécies. A maioria desses abrigos também era usada por pacas (Agouti paca Linnaues, 1766, capivaras (Hydrochaeris hydrochaeris, Linnaues, 1766, guaxinins (Procyon cancrivorus, G. Cuvier, 1798 e raposas (Lycalopex vetulus, Lund, 1842 que são suscetíveis à raiva. Em 546 Desmodus

  12. Quantitative Classification of Epiphytic Lichen Communities in Tumur Peak National Natural Reserve%新疆托木尔峰国家级自然保护区树附生地衣群落数量分类1)

    Institute of Scientific and Technical Information of China (English)

    艾尼瓦尔·吐米尔; 阿不都拉·阿巴斯

    2015-01-01

    在托木尔峰国家级自然保护区(海拔2200~3000 m)设置25个20 m×20 m的样地,调查样地内树附生地衣种类、地衣盖度和生境指标。以地衣种盖度为指标,应用除趋势对应分析法( DCA)和双向指示种分析法( TWINSPAN)对树附生地衣群落进行数量分类。根据数量分类结果并综合生境特征,将保护区树附生地衣划分为4个群落类型。群落A:茎口果粉衣( Chaenotheca stemonea ( Ach.) Muell)+黑蜈蚣衣( Phaeophyscia nigricans ( Flk.) Moberg)+枪石蕊群落(Cladonia coniocraea (Floerke) Spreng.);群落B:黄烛衣(Candelaria concolor (Dicks.) Stein.)+粉粒树花( Ramalina pollinaria ( Westr.) Ach).+中国树花( R.sinensis Jatta.);群落C:柯贝尔副茶渍( Lecania koerberi-ana Lahm.)+金黄茶渍( Candelariella aurella ( Hoffm.) Zahlbr.)+粗糙猫耳衣( Lepot gium brebisonii Mont.apud Webb.);群落D:斑面蜈蚣衣( Physcia aiop lia ( Humb.) Furnr.)+类锈橙衣( Caloplaca ferrugineoides ( H.Magn))+污白雪花衣( Anophychia ulto hir coied s ( Vain.) Vain.)。群落B和群落C的相似性最高,相似性系数为0.918;群落C和群落D的相似性系数为0.649;群落A与群落D相似性系数最低,相似性系数为0.264。树附生地衣的分布与海拔的关系较密切,高海拔和低海拔地带树附生地衣群落物种组成具有显著性差异。%We set 25 quadrats of 20 m×20 m at different altitudes along an altitudinal gradient of 2 200-3 000 m in Tumur Peak National Natural Reserve in Xinjiang .We identified lichen species in each quadrat and measured the lichen coverage of the quadrat combined with several environmental indices .We used the detrended correspondence analysis ( DCA) and two-way indicator species analysis (TWINSPAN)methods to classify the epiphytic lichens communities

  13. Ectima contagioso em ovinos e caprinos no semi-árido da Paraíba Contagious echtyma in sheep and goats in the semiarid of Paraíba, Brazil

    Directory of Open Access Journals (Sweden)

    Jandui E. Nóbrega Jr

    2008-03-01

    Full Text Available Descrevem-se dez surtos de ectima contagioso em caprinos e dois em ovinos no semi-árido da Paraíba. Os animais jovens foram os mais afetados, mas em oito surtos animais adultos também foram afetados, principalmente cabras lactantes que apresentavam lesões no úbere. Em nenhum dos rebanhos afetados havia antecedentes de vacinação contra ectima contagioso. Histologicamente a epiderme apresentava acantose com hiperplasia pseudoepiteliomatosa e com múltiplos focos de degeneração hidrópica formando vesículas ou pústulas. Inclusões intracitoplasmáticas eosinofílicas foram observadas em queratinócitos. Havia restos celulares necróticos formando crostas, hiperqueratose e microabscessos intracorneais ou extensas áreas ulceradas. Na derme observou-se infiltrado inflamatório mononuclear difuso. Também foi registrado um caso humano em um pesquisador que tinha administrado planta por via oral a um caprino que, posteriormente, apresentou lesões de ectima contagioso. Neste caso, também foram observadas lesões características em uma biopsia e o vírus foi identificado por microscopia eletrônica. Conclui-se que a doença é endêmica no semi-árido e que é necessária a vacinação sistemática dos rebanhos para diminuir os prejuízos econômicos causados pela mesma. A utilização de luvas para o manuseio de animais afetados é necessária para evitar casos humanos da enfermidade.Ten outbreaks of contagious ecthyma in goats and two in sheep in the semiarid of Paraíba State are reported. Young animals were more frequently affected, but in 8 outbreaks adult animals were also affected, mainly lactating goats which developed udder lesions. None of the affected flocks had been vaccinated. Upon histologic examination the epidermis showed acanthosis with pseudoepitheliomatous hyperplasia and multifocal ballooning degeneration forming vesicles or pustules. Eosinophilic inclusion bodies were observed in keratinocytes. Crust of necrotic cells

  14. Evaluation of morphological changes of the skin after radiation-induced injury in Wistar rats;Avaliacao de alteracoes morfologicas da pele apos lesao radioinduzida em ratos Wistar

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Cherley Borba Vieira de

    2010-07-01

    irradiated area. The optical microscope verified the early development of lesions in the 5 days group. After 10 days of irradiation it was already possible to see evidences of epidermal healing under the crust formed by the lesion. At 15 days after irradiation the tissue beneath the lesion had reconstructed epidermis. It was also observed the characteristics of tissue healing. It was also seen a significant polymorphonuclear cells infiltration. After 25 days at the higher doses the lesions remained, which did not occur at the lower dose, where the irradiated area of the animals were already completely healed. After 100 days of irradiation at 40Gy dose the wound was healed with the emergence of a well defined healed tissue. At the 60Gy dose, the lesion persisted in some animals, and in those that healing occurred the epidermis was a hypertrophied (acanthosis). It was visualized a tissue with their morphological aspect completely mis characterized, where it's possible to view a necrotic tissue. The results of the scanning electron microscopy analysis corroborate with the in optical microscopy findings, where it's possible to visualize the distortion of collagen fibers at the higher dosages. The results indicate that the used doses induced an important skin inflammatory process, activating the immune system. This fact leads to an increase in the expression of TGF beta1, one of the responsible for the increased production of extracellular matrix (ECM) by various cell types, mainly fibroblasts in injured tissues. Besides the increased expression of ECM, the TGF beta1 also promotes the inhibition of the degradation processes of the same. The intense expression of cytokines in irradiated skin can trigger the fibrosis process; consequently, affect the homeostasis of this organ due to the accumulation of ECM. (author)

  15. 获得性指(趾)部纤维角化瘤和获得性甲周纤维角化瘤的临床和病理分析%The clinical and pathological analysis of 6 cases of acquired digital fibrokeratoma and acquired periungual fibrokeratoma

    Institute of Scientific and Technical Information of China (English)

    狄梅; 朱英

    2012-01-01

    目的 探讨获得性指(趾)部纤维角化瘤(ADF)和获得性甲周纤维角化瘤(APF)的关系.方法 对ADF和APF各3例的临床表现、组织病理学及治疗结果进行对比分析.结果 患者均为女性,以指趾部指状新生物为主诉就诊.病程0.5 ~10年.3例ADF损害为指部肤色外生性肿物,短指状,顶部圆钝,基底部皮肤呈衣领状包绕.3例APF分别发生在甲板与甲小皮之间及甲板上,长轴与侧甲沟走行一致,顶部角化,质硬,损害下甲板形成纵行凹陷.6例组织病理学检查均示表皮角化过度,棘层肥厚,真皮可见大量增生的胶原纤维及成纤维细胞,弹力纤维减少,其中APF增生胶原排列与损害长轴一致.ADF可行激光、手术等治疗,APF则应手术治疗.结论 ADF和APF关系密切,APF是ADF的亚型,但因临床表现、组织病理及治疗方法均有其特点,所以发生于甲部的ADF诊断为APF更好.%Objective To investigate the relation between acquired digital fibrokeratoma (ADF)and acquired periungual fibrokeratoma(APF).Methods Clinical features,histopathology and treatment of 3 cases of ADF and 3 cases of APF were studied.Results All patients were female with digital neoplasm without symptom for half to ten years.The lesion of ADF was skin color and digital shape neoplasm on finger with a round top and a collarette of slightly raised skin that encircles the base of the lesion.While the lesion of APF is firm,keratotic projection located beneath the eponychium or on nail plate that parallel to the lateral nail groove.The nail plate had a shallow concave depression under the lesion.The common histopathologic findings included connective tissue proliferation surrounded by an acanthosis band of epidermis with hyperkeratosis.The special staining showed thin and reduced elastic fibres in the dermis.Proliferation of connective tissue in APF was cylindriform parallel to the lesion.ADF could be treated by laser and surgery operation while APF should be

  16. The Mice Drawer System (MDS) Tissue Sharing Programme: effect of space conditions on skin metabolic activity and vascularization and potential impact of radiations in mice.

    Science.gov (United States)

    Nusgens, Betty; Lambert, Charles; Liu, Yi; Cancedda, Ranieri; Tavella, Sara; Ruggiu, Alessandra; Colige, Alain

    morphological and immunochemical analysis aim at investigating dermal and epidermal thickness, number and surface of blood vessels (CD31, VW factor, type IV collagen) and lymphatics (D2-40, Lyve), score of acanthosis and papillomatosis, proliferation index (Ki67), epidermal markers of differentiation and FXIIIa positive dendritic cells. Another fragment of skin is used for measuring water content, total collagen content (hydroxyproline) and total proteoglycans content (uronic acid). A differential extraction procedure allows to quantify the newly synthetized collagen and the progressively more heavily cross-linked colla-gen, the pattern of extraction reflecting the turn-over rate of collagen. Quantitative RT-PCR procedure is used to evaluate the expression of extracellular matrix components (fibrillar and FACIT collagens), the enzymes involved in their postranslational modifications (ADAMTS-2, -3, -14, BMP1, prolylhydroxylase, lysyloxydase), the MMPs and their physiologic activators and inhibitors, proteoglycans of the hyalectans family and the SLRP, hyaluronansynthase, ag-grecanases of the ADAMTS family, and vascular markers (CD31, VEGF-A, -C, -D, VEGF-R1, -R2, -R3, PlGF, NRP1 2, SEMA3). A special attention will be paid to the splice variants of VEGF-A. We recently discovered a new splice variant, VEGF111, lacking exons 5, 6 and 7, bio-logically active and resistant to proteolysis that is specifically induced by genotoxic agents such as radiations. Its expression might reflect an impact of radiations during the long stay of the animals in space environment. Additionally, a genome-wide analysis of gene expression will be performed using DNA microarrays (Affimetrix). Partial results of the analyses under way will be presented. http://www.nasa.gov/missionp ages/station/science/experiments/M DS.html

  17. Evaluation of anatomical and functional changes esophageal stump of patients with advanced megaesophagus submitted to subtotal laparoscopic esophagectomy Avaliação das alterações anatômicas e funcionais do coto esofágico de pacientes portadores de megaesôfago avançado submetidos à esofagectomia subtotal laparoscópica

    Directory of Open Access Journals (Sweden)

    Júverson Alves Terra Júnior

    2012-09-01

    Full Text Available PURPOSE: Evaluate anatomical and functional changes of the esophageal stump and gastric fundus of patients with advanced megaesophagus, submitted to laparoscopic subtotal esophagectomy. METHODS: Twenty patients with advanced megaesophagus, previously submitted to a videolaparoscopic subtotal esophagectomy , were evaluated. Were conducted: radiological evaluation of the stump esophagus with transposed stomach, electromanometric, endoscopic examination and histopathology of the esophageal stump and gastric fundus, without making gastric tube or pyloroplasty. RESULTS: It was observed that the average height and pressure of the anastomosis, in the electromanometric evaluation, were 23.45cm (±1.84cm and 7.55mmHg (±5.65mmHg. In patients with megaesophagus III, the pressure of the anastomosis was 10.91mmHg (±6.33mmHg, and pressure from the UES, 31.89mmHg (±14.64mm Hg, were significantly higher than those in grade IV. The pathological evaluation detected mild esophagitis in 35% of patients, moderate in 20% and acanthosis glicogenica in 45%. The examination of the gastric fundus showed that 50% of patients were infected with Helicobacter pylori. Chronic gastritis occurred in 95% of the patients. CONCLUSIONS: The laparoscopic esophagectomy shown to be effective in the treatment of advanced achalasia. The cervical level anastomosis protects the esophageal stump from the aggression resulted from gastric reflux after the esophagectomy.OBJETIVO: Avaliar as alterações anatômicas e funcionais do coto esofágico e fundo gástrico de pacientes com megaesôfago avançado, submetidos à esofagectomia subtotal laparoscópica. MÉTODOS: Vinte pacientes com megaesôfago avançado, previamente submetidos à esofagectomia subtotal videolaparoscópica, foram avaliados. Foram realizados: avaliação radiológica do coto esofágico com o estômago transposto, eletromanometria endoscopia e exame histopatológico do coto esofágico e fundo gástrico,sem fazer tubo g

  18. Leucoplasia pilosa oral: aspectos histopatológicos da fase subclínica Oral hairy leukoplakia: histopathologic features of the subclinical stage

    Directory of Open Access Journals (Sweden)

    Eliane Pedra DIAS

    2001-06-01

    characterization. The present study had the aim to describe the histopathological characteristics of subclinical hairy leukoplakia, as well as to carry out a comparative analysis between clinical and subclinical OHL. For that, 11 cases were analyzed - 5 biopsies from patients who presented with the lesion and 6 samples from the borders of tongues obtained in necropsies. The histopathological findings in subclinical OHL were: absence of parakeratosis and papillomatosis, mild acanthosis, ballooning cells and nuclear alterations. In situ hybridization and immunostaining were positive for EBV in the nuclear alterations identified in the histophatological analysis. Based on the identification of EBV in the nuclear alterations, it was possible to conclude that subclinical OHL, similarly to the clinical lesion, presents histopathological features that are specific and sufficient to establish the definitive diagnosis, regardless of the identification of the virus.

  19. Symmetric acral keratoderma: 9 case reports%对称性肢端角化病九例分析

    Institute of Scientific and Technical Information of China (English)

    张志扬; 郑晓晖

    2011-01-01

    Objective To investigate the relevant risk factors for,clinical manifestation and treatment responses of symmetric acral keratoderma in 9 patients.Methods Clinical data were collected from 9 outpatients with symmetric acral keratoderma.Skin lesions were photographed,tissue specimens were obtained from the lesions and subjected to fungal and histopathological examinations.The patients were given topical treatment.Results There were 8 males and 1 female in these patients with an average age of 28.2 years and age at onset of 24.6 years.The disease duration varied from 2 months to 16 years.The lesions were predominantly distributed on the dorsum of both hands and wrists symmetrically and circularly.Ankles were the third popular site involved.Generally,the lesions were aggravated in summer,spontaneously cured in winter.Most of the patients had a history of occupational contact with plastics or rubber.Some patients suffered from ichthyosis vulgaris.Microscopic examination for fungal element was negative.Histopathology revealed epidermal hyperkeratosis,acanthosis and mild papillomatosis.The urea and Vitamin E cream mixed with clobetasol propionate cream at a ratio of 1 ∶ 1 proved to be effective.Conclusions Ichthyosis vulgaris may be a risk factor for symmetric acral keratoderma.The whitening of lesions after short exposure to water may be a kind of physical phenomenon with the involvement of water and air.Topical steroids may serve as the first-line treatment choice for symmetric acral keratoderma.%目的 探讨9例对称性肢端角化病患者的相关危险因素、临床表现、治疗及疗效.方法 门诊收集9例对称性肢端角化病患者的临床资料.对皮损进行拍照,真菌镜检、组织病理检查.予外用药治疗并随访.结果 男8例,女1例,平均年龄28.2岁,平均发病年龄24.6岁,病程2个月至16年;皮损对称分布,双手背、腕全部累及,踝次之;手腕部皮损分布呈圈状;夏季重、冬秋季多数自愈;

  20. ANATOMIC-PATHOLOGICAL STUDY OF DIGITAL DERMATITIS WOUNDS IN CATTLE ESTUDO ANATOMOPATOLÓGICO DE LESÕES DE DERMATITE DIGITAL EM BOVINOS

    Directory of Open Access Journals (Sweden)

    Camila França de Paula Orlando

    2008-12-01

    Full Text Available This essay had the scope of characterizing anatomic-pathological aspects of digital dermatitis wounds in distinct development phases. The essay was carried out from August 2004 and November 2005, using 40 cows distributed in four groups of ten animals each. Group I (GI consisted of clinically healthy animals, and groups II (GII, III (GIII and IV (GIV, consisted of animals bearing wounds in initial, erosive and proliferative phases, respectively. In GII histological observations were corneal stratus’ thickening, necrosis, hiperplasia, acanthosis, espongiosis and hyperqueratosis. In GIII it was observed hyperemia, ulcers, granulation tissue, hemorrhage and, microscopically, corneal layer thickening, paraqueratosis and multifocal necrosis. In GIV lesions of verrucous aspect, presence of fur, papillary projections were observed macroscopically, and, at histological examination, destruction of corneal layer and epidermis along with necrosis in all samples from the group.  Concerning the microbiota, fungi were not observed in any group. As for bacteria, mixed flora was observed, especially spirochetes, in GII, GIII e GIV. Finally, it was concluded that histological alterations in distinct phases of digital dermatitis may commonly be present, differing in terms of severity. The presence of spiral microorganisms suggests their association with etiopathogenesis.

    KEY WORDS: Cattle, digital lesions erosives and proliferatives, histopathology. Este trabalho objetivou caracterizar os aspectos anatomopatológicos das lesões de dermatite digital em diferentes fases evolutivas. O estudo ocorreu entre agosto de 2004 e novembro de 2005, utilizando-se quarenta vacas alocadas em quatro grupos, contendo dez animais cada. O grupo I (GI foi composto por animais clinicamente saudáveis e os grupos II (GII, III (GIII e IV (GIV, com lesões na fase inicial, erosiva e proliferativa, respectivamente. No GII, à histologia observaram-se espessamento do

  1. Blau综合征一例%Blau syndrome: a case report and review of literature

    Institute of Scientific and Technical Information of China (English)

    陈宏; 常晓萍

    2008-01-01

    A 4-year-old girl presented with generalized papules, erythema, desquamation, subcuta-neousnodules around large joints, as well as a 3-year-history of progressive eye damage. Three months after her birth, papules developed on the lower limbs, and gradually spread to involve the whole body. Eye damage appeared after a high fever and she was diagnosed with keratoleukoma, old iritis, and posterior synechia. Physical examination revealed sharply demarcated corneal opacity, shallow anterior chamber, obscure irides, and posterior synechia; pupils were not round. No superficial lymphadenectasis was observed. Dermatological examination showed ichthyosis-like coarse skin with generalized papules, erythema and desquamation, increased wrinkles and absence of sweat. Skin temperature was higher than normal. There were soft, irregu-larly sized, non-tender, movable subcutaneous masses at the wrist, ankle and knee joints without inflamma-tion or warmth. Type-B uitrasonography of both eyes disclosed vitreous opacification, posterior detachment of vitreous, extravasation at the posterior vitreous membrane, and decreased intraocular pressure. Histopathology of lesions on the back, waist, and back of hands as well as subcutaneous nodules on the wrists showed epidermal acanthosis and infiltration with a large number of histiocytes, foam cells and multi-nucleated giant cells in the dermis. No abnormal findings were observed from other examinations. This case was diagnosed as Blau syndrome, which is an autosomal dominant inherited disease; currently, there is no effective therapy for it.%患儿女,4岁,全身皮肤弥漫性丘疹、红斑、脱屑,大关节处皮下结节伴进行性眼损害3年.患儿出生后3个月出现下肢丘疹,逐渐累及全身.一次高热后出现眼部病变,诊断为双角膜白斑,陈旧性虹膜炎,虹膜后粘连.体检:双角膜散在灰白色混浊,边界清,前房浅,虹膜斑不清,瞳孔不圆,有后粘连.全身浅表淋巴结不大.皮肤科检

  2. Investigation on the universality of antibiotic property of polysaccharide in lentinan%香菇多糖抗菌抗病毒普适性研究

    Institute of Scientific and Technical Information of China (English)

    侯爱萍; 张树梅

    2015-01-01

    目的:探讨香菇多糖对细菌、真菌和病毒的抑制作用。方法细菌(溶血性链球菌、金黄色葡糖球菌、鼠伤寒沙门氏菌、枯草芽孢杆菌、大肠杆菌、多杀性巴氏杆菌、痢疾志贺氏菌、伤寒杆菌、甲型副伤寒杆菌)和真菌(白色念珠菌、啤酒酵母、红色毛癣菌、青霉、绿色木霉、黑根霉、烟曲霉)做成含菌平板,放置含有一定浓度香菇多糖的滤纸片,培养后通过测量抑菌圈计算最低抑菌浓度。对病毒(流感病毒、呼吸道合胞病毒、腺病毒、柯萨奇病毒A、单纯疱疹病毒1型、轮状病毒、埃可病毒2型)的抑制作用通过多糖先和细胞作用及先和病毒作用两种方式。结果香菇多糖对9种供试细菌和7种病毒都有抑制作用,只对7种供试真菌中的3种有微弱抑制作用。结论香菇多糖对细菌和病毒具有普遍抑制作用,而对真菌无普遍抑制作用。%Objective To investigate the general inhibitive effect of lentinan on bacteria,fungi and viruses. Methods Double layer agar plates were cultured with the following bacterias:Hemolytic streptococcus,Staphylococcus aureus glucose, Salmonella typhimurium,Bacillus subtilis,E. Coli,Pasteurella multocida,Shigella dysentery,Salmonella typhi,Paratyphoid bacilli and following fungi:Candida albicans,Brewer's yeast,Trichophyton rubrum,Penicillium,Viride,Rhizopus nigricans,As-pergillus fumigates. Filter paper with known concentration lentinan was put on top of the agar plates. After culture for 24 hours the minimal concentration of polysaccharide which inhibits bacterial/fungal growth was calculated by measurement of the size of inhibitive ring. The inhibition on viruses( influenza virus,respiratory syncytial virus,adenovirus,coxsackie virus A,herpes simplex virus-1,rotavirus,ECHO virus-2)was investigated by binding of lentinan to either the host cells or di-rectly to viruses. Results Lentinan showed inhibitive effect on all nine

  3. Ecologia de populações de morcegos cavernícolas em uma região cárstica do sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Eleonora Trajano

    1984-01-01

    the same caves as D. rotundus, whereas Puripterus horrens and A. caudifer avoid them. Common species showed a «sunset-related» timing of flight activity. The emergence of the majority of the populations begins at dusk, but the activity peak at cave entrance varies in timing and duration. The strictly insectivorous species (Peropterix macrotis, Myotis nigricans and P. horrens and also A. caudifer leave the roost earlier, showing an emergence peak during the crepuscle; for the others, the peak occurs after darkening. A few species, such as D. rotundus and Lonchorhina aurita, emerge only after total darkness. The use of temporary, nocturnal roosts seems to be frequent in the Upper Ribeira, occurring during or after foraging activities; individuals of A. lituratus can use caves as ingesting places. C. perspicillata finishes its feeding activities earlier than the other common species, which indicates a high foraging efficiency. With few exceptions, bats of the Upper Ribeira do not carry their young during foraging. The supply of food for the hematophagous species is represented by small and medium sized domestic animals (poultry and pigs; wild animals may also be significant as prey.

  4. A study of waterbirds wintering in Mission Bay combining field methods and GIS

    Science.gov (United States)

    Handa, Lesley Mika

    natural area size. Favorable conditions occurred mid-winter for Eared Grebe (Podiceps nigricollis ) and Pacific Black Brant (Branta bernicla nigricans) as the height of the population appeared early in winter. Presence and activity of shorebirds were associated with tidal cycle with shorebirds foraging at low tide and non-foraging activities at high tide. Cooler water temperature was preferred across several bird groups including Black Brant which may be in response to eelgrass fluctuation. Overall, more bird abundance and biodiversity were present the second year compared to the first. Twenty-two waterbird species out 72 of the total species documented during the project used man-made resources during winter and this may be at an advantage in the future compared to more sensitive species as wetland loss occurs with climate change and the increase in urbanization in natural areas. Increasing wetland area through ongoing restoration and reproduction of wetland habitat of similar quality may maintain or increase species richness with a higher carrying capacity of waterbird numbers present today.

  5. 不同菌种发酵制备蚕豆酱的研究

    Institute of Scientific and Technical Information of China (English)

    刘莹; 胡茂丰; 刘素纯; 王巨涛; 吴章华

    2014-01-01

    The effect of A spergillusoryzae,A spergillusniger,R hizopusnigricans and Mucorm ucedo ferm entation on protease activity,am ylase activ-ity,nutritionalingredientsand O D 420nm value ofbroad bean paste w ere determ ined,and sensory evaluation of the paste w asconducted atthe 42 d.The results showed that,neutral and alkaline protease activity of A. oryzae was stronger than the otherthree m oulds,w hich w ere 546.84 U and 188.79 U , respectively.The am ino acid nitrogen contentferm ented by each moulds in order was A. oryzae, R. nigricans, A. nigerand M. mucedo, and the con-tentin A. oryzae-ferm ented broad bean paste was 1.381 g/100 g.The pastesferm ented by A. oryzae and A .nigerw ere darkerw ith higherO D 420nm val-ue, while the pastesferm ented by the othertw o muldsw ere lighterw ith low erO D 420nm value.The paste ferm ented by A. oryzae reached the first-level standard in color,flavorand taste,and it was the optimalmould in broad bean paste-making.%本试验研究米曲霉、黑曲霉、黑根霉、高大毛霉4种不同单一菌种分别发酵制得的蚕豆酱中蛋白酶、淀粉酶活力及主要成分、酱色O D 420nm值等指标在不同发酵时期的变化,并对发酵42 d的蚕豆酱进行感官评价。结果表明,米曲霉的中性及碱性蛋白酶活力较其他三种菌强,分别高达546.84 U 和188.79 U 。米曲霉发酵的蚕豆酱氨基酸态氮含量最高,可达1.381 g/100 g,根霉、黑曲霉次之,毛霉最少。米曲霉和黑曲霉制得酱的颜色深,酱色O D 420nm值高,另两种霉制得酱的颜色较浅。米曲霉发酵制得蚕豆酱在色泽、气味和口感上都达到一级标准,是制作蚕豆酱的良好发酵菌种。

  6. NTP Toxicology and Carcinogenesis of 1,2,3-Trichloropropane (CAS No. 96-18-4) in F344/N Rats and B6C3F1 Mice (Gavage Studies).

    Science.gov (United States)

    1993-08-01

    to chemical administration. The principal toxic lesions associated with the administration of 1,2,3-trichloropropane to rats were hepatocellular necrosis, karyomegaly, and biliary hyperplasia of the liver; renal tubule necrosis, regeneration, and karyomegaly of the kidney; and necrosis and inflammation of the nasal olfactory and respiratory epithelium. Groups of 20 male and 20 female mice received 1,2,3-trichloropropane in corn oil by gavage at doses of 8, 16, 32, 63, 125, or 250 mg/kg 5 days per week for up to 17 weeks; 30 male and 30 female mice received corn oil alone and served as controls. Sixteen male and seven female mice in the 250 mg/kg groups died by week 4. The final mean body weights and mean body weight gains of dosed mice were similar to those of the controls, except those of 250 mg/kg males, which were lower than those of controls. The principal toxic lesions associated with the administration of 1,2,3-trichloropropane were hepatocellular necrosis and karyomegaly of the liver; necrosis, regeneration, and hyperplasia of the bronchiolar epithelium in the lung; and acanthosis (hyperplasia) and hyperkeratosis of the forestomach epithelium. 2-Year Studies: Groups of 60 male and 60 female rats received 0, 3, 10, or 30 mg 1,2,3-trichloropropane/kg body weight in corn oil by gavage 5 days per week for up to 104 weeks. Selection of 30 mg/kg as the high dose in these studies was based on the following chemical-related effects in the 17-week studies: deaths and liver and kidney lesions at 125 and 250 mg/kg and reduced final mean body weights and mean body weight gains at 63 mg/kg or greater. Groups of 60 male and 60 female mice received 0, 6, 20, or 60 mg 1,2,3-trichloropropane/kg body weight in corn oil by gavage 5 days per week for up to 104 weeks. Selection of 60 mg/kg as the high dose was based on chemical-related deaths and lesions of the liver, lung, and forestomach at 125 and 250 mg/kg in the 17-week studies. 15-Month Interim Evaluations: Up to 10 rats and

  7. NTP Toxicology and Carcinogenesis Studies of o-Phenylphenol (CAS No. 90-43-7) Alone and with 7,12-Dimethylbenz(a)anthracene (CAS No. 57-97-6) in Swiss CD-1 Mice (Dermal Studies).

    Science.gov (United States)

    1986-03-01

    decrease in survival in the positive control group (DMBA/TPA). Skin neoplasms classified as squamous cell papillomas, squamous cell carcinomas, basal cell tumors, basal cell carcinomas, keratoacanthomas, or sebaceous adenomas occurred in mice dosed with DMBA/acetone, DMBA/o-phenylphenol, or DMBA/TPA alone. However, the incidence of skin neoplasms in mice dosed with DMBA/acetone (15/100) was similar to that in mice dosed with DMBA/o-phenylphenol (17/100). The incidence of skin neoplasms in male and female mice dosed with DMBA/TPA (52/100) was substantially greater than those in mice dosed with either DMBA/acetone or DMBA/o-phenylphenol. Similarly, the mean time of appearance of skin papillomas occurred much earlier in the DMBA/TPA groups than in the DMBA/acetone or DMBA/o-phenylphenol groups. All groups had nonneoplastic lesions consisting of inflammation, ulceration, hyperkeratosis, and acanthosis at the site of application. These lesions were present in the acetone vehicle control group and, to a larger extent, in the o-phenylphenol, DMBA/o-phenylphenol, and DMBA/TPA groups. No skin neoplasms were observed in male or female mice receiving o-phenylphenol or in the acetone vehicle control groups. Moreover, a complete histopathologic review revealed no other neoplasms at any other site at significantly increased incidences in the groups receiving o-phenylphenol compared with the acetone vehicle controls. There were also no tumor-enhancing (or tumor-inhibiting) effects of o-phenylphenol and DMBA given in combination. o-Phenylphenol was weakly mutagenic in strains TA1535 of Salmonella typhimurium only in the absence of rat liver S9; it was not mutagenic in strains TA1537, TA98, or TA100. It was mutagenic in the mouse lymphoma L5178/TK+/- assay in the presence or absence of Aroclor 1254-induced male F344 rat liver S9. o-Phenylphenol did not induce sex-linked recessive lethal mutations in Drosophila melanogaster. o-Phenylphenol induced sister-chromatid exchanges in Chinese

  8. Observaciones ecológicas sobre los peces Bocachico real (Prochilodus mariae eigenmann 1922 y el Bocachico cardumero (Suprasinelepichthys laticeps valenciennes 1849 del sistema del rio metica y algunos datos comparativos del Bocachico (Prochilodus r

    Directory of Open Access Journals (Sweden)

    Flórez A. Fabio

    1986-06-01

    Full Text Available In this investigation, comparative ecological data of three species of freshwater fish is presented. Two of them belong to the Metica river system, the Bocachico Real (Prochilodus mariae Eigenmann 1922 and the Bocachico Cardumero (Suprasinelepichthys laticeps Valenciennes 1849. The Prochilodus reticulatus magdalenae Steindachner 1818,
    Bocachico to the Magdalena river system. lt was observed that the spawning period coincides with periud of
    greatest rainfall. A decrease of the water superficial temperature is produced when the Metica's river meanders rains increase, such as it is sustained by Geisler, et al. (1973, for the genus Prochllodus in the Amazon river system.
    Differential distribution is given for species of Prochilodus as observed by Dahl, et al. (1963;, for Prochilodus r. magdalenae, and by Goulding, (1979for Prochilodus nigricans in Brazil. Induced reproduction of Prochilodus mariae was obtained, and its reproductive behaviour very similar ro the Prochllodus r. magdalenae (Solano, 1973. The ratios of lenght and weight of the two Metica river species are
    compared, and there is a significant correlation in the ratio of the gonad's weight to the lenght, weight of the gonad and rnaturlty index of Supraslnelepichthys latlceps. The greatest fertility average corresponds to Prochilodus r. magdalenae
    with 355.535 ovum, followed by Prochilodus mariae with 215.111 and Suprasinelepichlhys laticeps with 158.667.
    The condition factor was determined for males and females, as well as the gonad index, maturity index and ovum diameter for the female.
    Datos ecológicos comparativos de tres especies de peces de agua dulce se presentan en la investigación, el bocachico real (Prochilodus marlae Eigenmann 1922, el bocachico cardumero (Suprasinelepichthys laticeps Valenciennes 1849 que pertenecen al sistema del alto río Metica y el bocachico (Prochilodus reticulatus magdalanea Steindachner 1878 del río Cauca, afluente

  9. Diabetes y Enfermedad Coronaria

    Directory of Open Access Journals (Sweden)

    Alfredo Jácome Roca

    1997-06-01

    aterogénesis en la diabetes tipo II? ¿Vale la pena hacer también un control estricto de hiperglicemia en el adulto o es contraproducente una “insulinización”? Vamos por partes.

    En 1988 Reaven describió el síndrome X: resistencia a la insulina, hiperinsulinismo, hipertensión, hipertrigliceridemia y HDL bajo, pero en personas delgadas También se describió una forma poco común de resistencia severa a la insulina por deficiencia congénita o por anticuerpos al receptor insulínico, asociada a Acantosis nigricans.

    Posteriormente se le añadió al síndrome X otro componente: la obesidad, pero la abdominal, básicamente la “visceral” (grasa de los epiplones, más que la subcutánea. Dicha obesidad abdominal es un factor de riesgo tanto para DMNID como para enfermedad coronaria y se asocia a resistencia insulínica lo que lleva a hiperinsulinismo por hipersecreción, para sobrepasar la resistencia; este es un círculo vicioso pues por el mecanismo de la “regulación hacia abajo”, a más insulina menos número de receptores, 10que lleva a alguna forma de hiperglicemia o de “disglicernía”, un nuevo término que luego explicaremos

    La DMNID tiene un riesgo para enfermedad cardiovascular (ECV de 2 a 4 veces mayor que en la población general, riesgo independiente de otros factores clásicos. El problema es que frecuentemente en estos diabéticos tipo 11 hay una resistencia a la insulina (como ahora se llama al antiguo síndrome X con todo su rosario de obesidad abdominal, hipertensión y dislipidemia.

    La DMNID o tipo 11 y la ECV tienen antecedentes genéticos y ambientales comunes. El bajo peso al nacer y la baja ganancia de peso en el primer año se asocian a la aparición de ambas entidades, décadas más tarde; lo que liga a las dos enfermedades es la resistencia a la insulina, definida por la presencia de 2 o más de los siguientes problemas: hipertensión, diabetes o intolerancia a la glucosa, hipertrigliceridemia y HDL bajo

  10. Taxonomia da subfamília Corinninae (Araneae, Corinnidae nas regiões neotropical e neártica Taxonomy of the subfamily Corinninae (Araneae, Corinnidae in neotropical and neartic regions

    Directory of Open Access Journals (Sweden)

    Alexandre Bragio Bonaldo

    2000-11-01

    . O. Pickard-Cambridge, C. nigricans (C. L. Koch, C. plumatus (L. Koch, C. praeceps (F. O. Pickard-Cambridge, C. silvaticus (Chickering, C. uncatus (F. O. Pickard-Cambridge, Erendira luteomaculatta (Petrunkevitch, E. pallidoguttata (Simon, E. subsignata (Simon, Falconina albomaculosa (Schmidt, F. crassipalpis (Chickering, F. gracilis (Keyserling, Megalostrata raptrix (L. Koch, Paradiestus egregius (Simon, P. giganteus (Karsch, P. penicillatus (Mello-Leitão, P. vitiosus (Keyserling, Septentrinna bicalcarata (Simon, S. paradoxa (F. O. Pickard-Cambridge, S. retusa (F. O. Pickard-Cambridge, Simonestus pseudobulbolus (Caporiacco, S. robustus (Chickering, S. semiluna (F.O. Pickard-Cambridge, Stethorrhagus maculatus (L. Koch and Xeropigo smedigari (Caporiacco; from Diestus, Corinna alticeps (Keyserling, C. kochi (Simon, Simonestus occidentalis (Schenkel, S. separatus (Schmidt and S. validus (Simon; from Lausus, Corinna grandis (Simon and Abapeba sicarioides (Mello-Leitão; from Medmassa, Corinna andina (Simon and C. venezuelica (Caporiacco; from Megalostrata, Erendira atrox (Caporiacco and Erendira pictitorax (Caporiacco; from Parachemmis, Tupirinna trilineata (Chickering. Five combinations are restaured: Corinna aenea Simon, Creugas cinnamius Simon, Creugas gulosus Thorell, Falconina melloi (Schenkel, Paradiestus aurantiacus Mello-Leitão. Twenty five new synonymies are proposed: Diestus altifrons Mello-Leitão with Corinna nitens (Keyserling; Corinna tomentosa Simon, C. tridentina Mello-Leitão, Hypsinotus flavipes Keyserling, H. humilis Keyserling and Xeropigo scutulatus Simon with Xeropigo tridentiger (O. Pickard-Cambridge; Corinna cribosa Mello-Leitão and C. stigmatica Simon with Falconina gracilis (Keyserling; Corinna casueta Chickering with SIMONestus separatus (Schmidt; Corinna abnormis Petrunkevitch, C. antillana BRYANT, C. consobrina Simon, C. inornata Kraus, C. nervosa F. O. Pickard-Cambridge, C. wolleboeki Banks, Creugas cetratus Simon, C. senegalensis Simon

  11. First report of the land planarian Diversibipalium multilineatum (Makino & Shirasawa, 1983) (Platyhelminthes, Tricladida, Continenticola) in Europe.

    Science.gov (United States)

    Mazza, Giuseppe; Menchetti, Mattia; Sluys, Ronald; Solà, Eduard; Riutort, Marta; Tricarico, Elena; Justine, Jean-Lou; Cavigioli, Luca; Mori, Emiliano

    2016-01-26

    , GTR+I+G, was estimated to be the best with the software jModeltest 2.1.7 (Darriba et al. 2012; Guindon & Gascuel 2003), using the Akaike Information Criterion (AIC). MrBayes analyses were performed for 10-milion generation with sampling parameters every 103 and a 25% default burn-in value for the final trees. Convergence of the two runs (average standard deviation of split frequencies http://www.geneious.com, Kearse et al. 2012) and were found to be identical. These results indicate that the species introduced in both countries is the same, and most probably concerns the species D. multilineatum. The pathways of introduction of D. multilineatum are currently unknown, although a relationship between the horticultural trade and the introduction of alien land planarians is well known (Álvarez-Presas et al. 2014 and references therein). Here we report the first occurrence of individuals of D. multilineatum outside Asia. The GenBank sequence of D. multilineatum from South Korea is not yet supported by a published description of the specimen, while it is debatable whether South Korea should be considered part of the natural range of D. multilineatum, which only seems to include Japan. In the present paper, we consider the South Korean animal to be an introduced specimen. Soil moisture status, temperature, and food availability are considered to be the main factors determining the presence of terrestrial planarians (Boag et al. 1998); the microclimatic conditions of the Italian garden were similar to plant nurseries and greenhouses, while an abundance of food was available, such as isopods [Porcellionides pruinosus (Brandt, 1833)], oligochaetes [Dendrobaena attemsi (Michaelsen, 1902) and several juveniles of Lumbricus spp.] and gastropods [Cernuella cisalpina (Rossmassler, 1837), Cornu aspersum (O.F. Müller 1774), Deroceras reticulatum (O.F. Müller, 1774), Discus rotundatus (O.F. Müller, 1774), Limacus flavus (Linnaeus, 1758), Milax nigricans (Philippi, 1836

  12. Plausibility of the implausible: is it possible that ultra-high dilutions ‘without biological activity’ cause adverse effects?

    Directory of Open Access Journals (Sweden)

    Marcus Zulian Teixeira

    2013-06-01

    intermediate to high propensity to produce at least one of the five symptoms that define ALTE, when given in homeopathic dilutions. Two of these (Veratrum album and Cuprum metallicum have an intermediate-to-high propensity to produce three of the four possible ALTE symptoms”. The authors conclude that “it is unlikely that the ALTE following ingestion of GCB was a toxic reaction to any of the drug’s component”, proposing the ‘homeopathic theory’ (pathogenetic manifestations [15] as explanation for this linkage. In view of these results, it can be inferred that AEs caused by homeopathic medicines at potencies ≥ 6C ‘are more closely related to the imponderable effects of ultra-high dilutions than the toxic (allergic effects of substances’. Accordingly, other case reports cited in that review described serious AEs with potencies of this magnitude, including the occurrence of ‘drug rash with eosinophilia and severe pulmonary involvement’ after using Sedativ PC (complex homeopathic medicine with 6 ingredients in 6CH [16], and the occurrence of ‘heart disease and bladder cancer’ with a complex homeopathic medicine in very high potencies (Aconitum napellus, Barytacarbonica, Cantharis vesicatoria, Gambogia, Pulsatilla nigricans and Rhus toxicodendron at 1000c, M or 10M potencies [17], discarding any influence of ‘toxicity of substances’. Similarly, in a systematic review on information regarding adverse effects of homeopathic medicines including 19 reports of clinical trials, 19 case (or case series reports and 15 homeopathic pathogenetic trials, Dantas and Rampes [18] concluded that “the mean incidence of AEs of homeopathic medicines (ultra-high dilutions was greater than placebo (9.4/6.1 in controlled clinical trials”, including mostly headaches, tiredness, skin eruptions, dizziness, bowel dysfunction such as diarrhea or loose stools and, more frequently, aggravations of symptoms following the administration of homeopathic medicines. Countering

  13. Resúmenes del Segundo Congreso Colombiano y Segundo Simposio Latinoamericano, Iberoamericano y Colombiano sobre Gastroenterología, Hepatología y Nutrición Pediátrica

    Directory of Open Access Journals (Sweden)

    2007-03-01

    á, ColombiaIntroducción: La enfermedad hepática grasa no alcohólica (NAFLD, en inglés «Non-Alcoholic Fatty Liver Disease» en niños obesos y su progresión a cirrosis, falla hepática y hepatocarcinoma es una consecuencia subestimada. No existen estudios que determinen su frecuencia en nuestro medio en niños. Objetivo: Determinar la frecuencia de hígado graso no alcohólico (NAFL mediante ultrasonográfía en un grupo de niños obesos. Pacientes y métodos: estudio prospectivo, descriptivo y de corte transversal, de 19 niños obesos (IMC >p95 según tablas de la NCHS entre 8-17 años (mayor prevalencia de la Fundación Cardio-Infantil. La presencia de hígado graso no alcohólico fue evaluado mediante ultrasonografía, previa autorización escrita. La infiltración grasa fue clasificada en grados del I-III. El estudio fue aprobado por el comité de bioética. Resultados: Encontramos una frecuencia de hígado graso no alcohólico (NAFL de 36.8%, mayor en el sexo masculino (71% y en pacientes con alto IMC (>p97, con antecedentes familiares de sobrepeso y/o diabetes tipo 2 (68% y 37% respectivamente, presencia de Acantosis nigricans (37% y transaminasas elevadas (AST 85% y ALT 100%. Conclusiones: Se encontró una alta frecuencia (36.8% de hígado graso mediante la ultrasonografía en estos niños obesos. Esta detección temprana evitaría complicaciones y su progresión. Se requieren estudios más amplios para determinar el diagnóstico y tratamiento más apropiado en niños.La nutrición en el escenario escolarFajardo AM, Gómez IM, Mondragón G, Corredor D, Ortegón J, Tovar LFacultad de Enfermería, Universidad El Bosque, Bogotá, ColombiaDesde las políticas colombianas sobre salud al colegio, se trabajó desde abril a diciembre del 2006 en una fundación que acoge niñas entre los 6 y los 20 años de edad que fomenta el desarrollo de actividades de educación y capacitación no formal, el fortalecimiento de principios y valores para la vida, como asistencia