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Sample records for abnormal laboratory results

  1. Pretest expectations strongly influence interpretation of abnormal laboratory results and further management

    NARCIS (Netherlands)

    Houben, P.H.; Weijden, G.D.E.M. van der; Winkens, B.; Winkens, R.A.G.; Grol, R.P.T.M.

    2010-01-01

    BACKGROUND: Abnormal results of diagnostic laboratory tests can be difficult to interpret when disease probability is very low. Although most physicians generally do not use Bayesian calculations to interpret abnormal results, their estimates of pretest disease probability and reasons for ordering d

  2. Pretest expectations strongly influence interpretation of abnormal laboratory results and further management

    Directory of Open Access Journals (Sweden)

    Winkens Ron AG

    2010-02-01

    Full Text Available Abstract Background Abnormal results of diagnostic laboratory tests can be difficult to interpret when disease probability is very low. Although most physicians generally do not use Bayesian calculations to interpret abnormal results, their estimates of pretest disease probability and reasons for ordering diagnostic tests may - in a more implicit manner - influence test interpretation and further management. A better understanding of this influence may help to improve test interpretation and management. Therefore, the objective of this study was to examine the influence of physicians' pretest disease probability estimates, and their reasons for ordering diagnostic tests, on test result interpretation, posttest probability estimates and further management. Methods Prospective study among 87 primary care physicians in the Netherlands who each ordered laboratory tests for 25 patients. They recorded their reasons for ordering the tests (to exclude or confirm disease or to reassure patients and their pretest disease probability estimates. Upon receiving the results they recorded how they interpreted the tests, their posttest probability estimates and further management. Logistic regression was used to analyse whether the pretest probability and the reasons for ordering tests influenced the interpretation, the posttest probability estimates and the decisions on further management. Results The physicians ordered tests for diagnostic purposes for 1253 patients; 742 patients had an abnormal result (64%. Physicians' pretest probability estimates and their reasons for ordering diagnostic tests influenced test interpretation, posttest probability estimates and further management. Abnormal results of tests ordered for reasons of reassurance were significantly more likely to be interpreted as normal (65.8% compared to tests ordered to confirm a diagnosis or exclude a disease (27.7% and 50.9%, respectively. The odds for abnormal results to be interpreted as

  3. Mean Abnormal Result Rate: Proof of Concept of a New Metric for Benchmarking Selectivity in Laboratory Test Ordering.

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    Naugler, Christopher T; Guo, Maggie

    2016-04-01

    There is a need to develop and validate new metrics to access the appropriateness of laboratory test requests. The mean abnormal result rate (MARR) is a proposed measure of ordering selectivity, the premise being that higher mean abnormal rates represent more selective test ordering. As a validation of this metric, we compared the abnormal rate of lab tests with the number of tests ordered on the same requisition. We hypothesized that requisitions with larger numbers of requested tests represent less selective test ordering and therefore would have a lower overall abnormal rate. We examined 3,864,083 tests ordered on 451,895 requisitions and found that the MARR decreased from about 25% if one test was ordered to about 7% if nine or more tests were ordered, consistent with less selectivity when more tests were ordered. We then examined the MARR for community-based testing for 1,340 family physicians and found both a wide variation in MARR as well as an inverse relationship between the total tests ordered per year per physician and the physician-specific MARR. The proposed metric represents a new utilization metric for benchmarking relative selectivity of test orders among physicians. © American Society for Clinical Pathology, 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  4. Abnormal Cervical Cancer Screening Test Results

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    ... FREQUENTLY ASKED QUESTIONS FAQ187 GYNECOLOGIC PROBLEMS Abnormal Cervical Cancer Screening Test Results • What is cervical cancer screening? • What causes abnormal cervical cancer screening test results? • ...

  5. Genetically modified laboratory mice with sebaceous glands abnormalities.

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    Ehrmann, Carmen; Schneider, Marlon R

    2016-12-01

    Sebaceous glands (SG) are exocrine glands that release their product by holocrine secretion, meaning that the whole cell becomes a secretion following disruption of the membrane. SG may be found in association with a hair follicle, forming the pilosebaceous unit, or as modified SG at different body sites such as the eyelids (Meibomian glands) or the preputial glands. Depending on their location, SG fulfill a number of functions, including protection of the skin and fur, thermoregulation, formation of the tear lipid film, and pheromone-based communication. Accordingly, SG abnormalities are associated with several diseases such as acne, cicatricial alopecia, and dry eye disease. An increasing number of genetically modified laboratory mouse lines develop SG abnormalities, and their study may provide important clues regarding the molecular pathways regulating SG development, physiology, and pathology. Here, we summarize in tabulated form the available mouse lines with SG abnormalities and, focusing on selected examples, discuss the insights they provide into SG biology and pathology. We hope this survey will become a helpful information source for researchers with a primary interest in SG but also as for researchers from unrelated fields that are unexpectedly confronted with a SG phenotype in newly generated mouse lines.

  6. The psychosocial impact of an abnormal cervical smear result.

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    Drolet, Mélanie; Brisson, Marc; Maunsell, Elizabeth; Franco, Eduardo L; Coutlée, François; Ferenczy, Alex; Fisher, William; Mansi, James A

    2012-10-01

    Data on the impact of abnormal cervical smear results on health-related quality of life (HrQoL) are scarce. We aimed to (i) prospectively assess the HrQoL of women who were informed of an abnormal smear result; (ii) identify predictors of greater negative psychosocial impact of an abnormal result; and (iii) prospectively estimate the quality-adjusted life-years (QALYs) lost following an abnormal result. Between 08/2006 and 08/2008, 492 women with an abnormal result and 460 women with a normal result, frequency matched for age and clinic, were recruited across Canada. HrQoL was measured at recruitment and 4 and 12 weeks later with the EuroQol, Short Form-12, short Spielberg State-Trait Anxiety Inventory (STAI) and HPV Impact Profile. Three blocks of potential predictors of higher psychosocial impact were tested by hierarchical modeling: (i) socio-demographics; (ii) sexual activity; and (iii) smear result severity, communication, and understanding. Receiving an abnormal result significantly increased anxiety (STAI mean difference between both groups = 8.3). Initial anxiety decreased over time for the majority of women. However, 35% of women had clinically meaningful anxiety at 12 weeks (i.e. STAI scores ≥0.5 standard deviation of the controls). These women reported a lower socio-economic level, did not completely understand the information about their result and perceived themselves at higher risk of cancer. QALY lost following an abnormal result were between 0.007 and 0.009. Receiving an abnormal smear has a statistically significant and clinically meaningful negative impact on mental health. However, this negative impact subsides after 12 weeks for the majority of women. Copyright © 2011 John Wiley & Sons, Ltd.

  7. Real-Time Microbiology Laboratory Surveillance System to Detect Abnormal Events and Emerging Infections, Marseille, France.

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    Abat, Cédric; Chaudet, Hervé; Colson, Philippe; Rolain, Jean-Marc; Raoult, Didier

    2015-08-01

    Infectious diseases are a major threat to humanity, and accurate surveillance is essential. We describe how to implement a laboratory data-based surveillance system in a clinical microbiology laboratory. Two historical Microsoft Excel databases were implemented. The data were then sorted and used to execute the following 2 surveillance systems in Excel: the Bacterial real-time Laboratory-based Surveillance System (BALYSES) for monitoring the number of patients infected with bacterial species isolated at least once in our laboratory during the study periodl and the Marseille Antibiotic Resistance Surveillance System (MARSS), which surveys the primary β-lactam resistance phenotypes for 15 selected bacterial species. The first historical database contained 174,853 identifications of bacteria, and the second contained 12,062 results of antibiotic susceptibility testing. From May 21, 2013, through June 4, 2014, BALYSES and MARSS enabled the detection of 52 abnormal events for 24 bacterial species, leading to 19 official reports. This system is currently being refined and improved.

  8. Abnormalities occurring during female gametophyte development result in the diversity of abnormal embryo sacs and leads to abnormal fertilization in indica/japonica hybrids in rice.

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    Zeng, Yu-Xiang; Hu, Chao-Yue; Lu, Yong-Gen; Li, Jin-Quan; Liu, Xiang-Dong

    2009-01-01

    Embryo sac abortion is one of the major reasons for sterility in indica/japonica hybrids in rice. To clarify the causal mechanism of embryo sac abortion, we studied the female gametophyte development in two indica/japonica hybrids via an eosin B staining procedure for embryo sac scanning using confocal laser scanning microscope. Different types of abnormalities occurred during megasporogenesis and megagametogenesis were demonstrated. The earliest abnormality was observed in the megasporocyte. A lot of the chalazal-most megaspores were degenerated before the mono-nucleate embryo sac stage. Disordered positioning of nucleus and abnormal nucellus tissue were characteristics of the abnormal female gametes from the mono-nucleate to four-nucleate embryo sac stages. The abnormalities that occurred from the early stage of the eight-nucleate embryo sac development to the mature embryo sac stage were characterized by smaller sizes and wrinkled antipodals. Asynchronous nuclear migration, abnormal positioning of nucleus, and degeneration of egg apparatus were also found at the eight-nucleate embryo sac stage. The abnormalities that occurred during female gametophyte development resulted in five major types of abnormal embryo sacs. These abnormal embryo sacs led to abnormal fertilization. Hand pollination using normal pollens on the spikelets during anthesis showed that normal pollens could not exclude the effect of abnormal embryo sac on seed setting.

  9. Abnormalities Occurring during Female Gametophyte Development Result in the Diversity of Abnormal Embryo Sacs and Leads to Abnormal Fertilization in indicaljaponica Hybrids in Rice

    Institute of Scientific and Technical Information of China (English)

    Yu-Xiang Zeng; Chao-Yue Hu; Yong-Gen Lu; Jin-Quan Li; Xiang-Dong Liu

    2009-01-01

    Embryo sac abortion is one of the major masons for sterility in indicaljaponica hybrids In rice. To clarify the causal mechanism of embryo sac abortion, we studied the female gametophyte development in two indicaljaponica hybrids via an eosin B staining procedure for embryo sac scanning using confocal laser scanning microscope. Different types of abnormalities occurred during megasporogenesis and megagamatogenesis were demonstrated. The earliest abnormality was observed in the megasporocyte. A lot of the chalazal-most megaspores were degenerated before the mono-nucleate embryo sac stage. Disordered positioning of nucleus and abnormal nucallus tissue were characteristics of the abnormal female gametes from the mono-nucleate to four-nucleate embryo sac stages. The abnormalities that occurred from the early stage of the eight-nucleate embryo sac development to the mature embryo sac stage were characterized by smaller sizes and wrinkled antipodals. Asynchronous nuclear migration, abnormal positioning of nucleus, and degeneration of egg apparatus were also found at the eight-nucleate embryo sac stage. The abnormalities that occurred during female gametophyte development resulted in five major types of abnormal embryo sacs. These abnormal embryo sacs led to abnormal fertilization. Hand pollination using normal pollens on the spikelets during anthesis showed that normal pollens could not exclude the effect of abnormal embryo sac on seed setting.

  10. Assessing the prevalence distribution of abnormal laboratory tests in patients with simple febrile seizure

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    Parsa Yousefichaijan

    2015-01-01

    Full Text Available Introduction: Febrile seizure is an important issue in pediatric practice. Even some pediatricians do not have a proper approach to febrile seizure, making the sick child undergo complex laboratory tests or invasive procedures or even long-term treatment with anticonvulsant drugs. In spite of multiple studies, many controversies have still remained about the significance of febrile seizure. The goal of this study is to assess the prevalence distribution of routinely requested laboratory tests results in simple febrile seizure. Materials and Methods: In a descriptive study, 549 patients with simple febrile seizure were studied. The routine lab tests including complete blood count, electrolyte, urine analysis, and cerebrospinal fluid (CSF analysis had already been performed for all patients and the results had been recorded in their medical data. These results were collected and statistically analyzed through SPSS software. Results: About 58.7% of our cases were male. Most of the cases were 12-24 months old and the mean body temperature of them was 38.2°C. 99.3% of blood sugar tests, 98% of blood calcium tests, 100% and 99.5% of sodium, and potassium tests, respectively, 100% of blood creatinine, 96.9% of blood urea nitrogen, and 99.1% of urine analysis tests were normal. CSF analysis was done in only 49 cases and the results were normal in all of them. Conclusion: The percentage of abnormal laboratory test results was not statistically significant in febrile seizure and shows that performing all these tests in all patients with simple febrile seizure as routine is not necessary.

  11. Assessing the prevalence distribution of abnormal laboratory tests in patients with simple febrile seizure.

    Science.gov (United States)

    Yousefichaijan, Parsa; Dorreh, Fatemeh; Abbasian, Ladan; Pakniyat, Abdol Ghader

    2015-01-01

    Febrile seizure is an important issue in pediatric practice. Even some pediatricians do not have a proper approach to febrile seizure, making the sick child undergo complex laboratory tests or invasive procedures or even long-term treatment with anticonvulsant drugs. In spite of multiple studies, many controversies have still remained about the significance of febrile seizure. The goal of this study is to assess the prevalence distribution of routinely requested laboratory tests results in simple febrile seizure. In a descriptive study, 549 patients with simple febrile seizure were studied. The routine lab tests including complete blood count, electrolyte, urine analysis, and cerebrospinal fluid (CSF) analysis had already been performed for all patients and the results had been recorded in their medical data. These results were collected and statistically analyzed through SPSS software. About 58.7% of our cases were male. Most of the cases were 12-24 months old and the mean body temperature of them was 38.2°C. 99.3% of blood sugar tests, 98% of blood calcium tests, 100% and 99.5% of sodium, and potassium tests, respectively, 100% of blood creatinine, 96.9% of blood urea nitrogen, and 99.1% of urine analysis tests were normal. CSF analysis was done in only 49 cases and the results were normal in all of them. The percentage of abnormal laboratory test results was not statistically significant in febrile seizure and shows that performing all these tests in all patients with simple febrile seizure as routine is not necessary.

  12. Long-Term Evaluation of Abnormal Behavior in Adult Ex-laboratory Chimpanzees (Pan troglodytes Following Re-socialization

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    Karl Crailsheim

    2013-01-01

    Full Text Available Adverse rearing conditions are considered a major factor in the development of abnormal behavior. We investigated the overall levels, the prevalence and the diversity of abnormal behavior of 18 adult former laboratory chimpanzees, who spent about 20 years single caged, over a two-year period following re-socialization. According to the onset of deprivation, the individuals were classified as early deprived (EDs, mean: 1.2 years or late deprived (LDs, mean: 3.6 years. The results are based on 187.5 hours of scan sampling distributed over three sample periods: subsequent to re-socialization and during the first and second year of group-living. While the overall levels and the diversity of abnormal behavior remained stable over time in this study population, the amplifying effects of age at onset of deprivation became apparent as the overall levels of abnormal behavior of EDs were far above those of LDs in the first and second year of group-living, but not immediately after re-socialization. The most prevalent abnormal behaviors, including eating disorders and self-directed behaviors, however, varied in their occurrence within subjects across the periods. Most important, the significance of social companionship became obvious as the most severe forms of abnormal behavior, such as dissociative and self-injurious behaviors declined.

  13. Lopinavir/ritonavir dosing during pregnancy in Brazil and maternal/infant laboratory abnormalities

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    Mario Ferreira Peixoto

    2011-06-01

    Full Text Available OBJECTIVES: To describe laboratory abnormalities among HIV-infected women and their infants with standard and increased lopinavir/ritonavir (LPV/r dosing during the third trimester of pregnancy. METHODS: We evaluated data on pregnant women from NISDI cohorts (2002-2009 enrolled in Brazil, who received at least 28 days of LPV/r during the third pregnancy trimester and gave birth to singleton infants. RESULTS: 164 women received LPV/r standard dosing [(798/198 or 800/200 mg/day (Group 1] and 70 increased dosing [(> 800/200 mg/day (Group 2]. Group 1 was more likely to have advanced clinical disease and to use ARVs for treatment, and less likely to have CD4 counts > 500 cells/mm³. Mean plasma viral load was higher in Group 2. There were statistically significant, but not clinically meaningful, differences between groups in mean AST, ALT, cholesterol, and triglycerides. The proportion of women with Grade 3 or 4 adverse events was very low, with no statistically significant differences between groups in severe adverse events related to ALT, AST, total bilirubin, cholesterol, or triglycerides. There were statistically significant, but not clinically meaningful, differences between infant groups in ALT and creatinine. The proportion of infants with Grade 3 or 4 adverse events was very low, and there were no statistically significant differences in severe adverse events related to ALT, AST, BUN, or creatinine. CONCLUSION: The proportions of women and infants with severe laboratory adverse events were very low. Increased LPV/r dosing during the third trimester of pregnancy appears to be safe for HIV-infected women and their infants.

  14. Effect of a laboratory result pager on provider behavior in a neonatal intensive care unit.

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    Samal, L; Stavroudis, Ta; Miller, Re; Lehmann, Hp; Lehmann, Cu

    2011-01-01

    A computerized laboratory result paging system (LRPS) that alerts providers about abnormal results ("push") may improve upon active laboratory result review ("pull"). However, implementing such a system in the intensive care setting may be hindered by low signal-to-noise ratio, which may lead to alert fatigue. To evaluate the impact of an LRPS in a Neonatal Intensive Care Unit. Utilizing paper chart review, we tallied provider orders following an abnormal laboratory result before and after implementation of an LRPS. Orders were compared with a predefined set of appropriate orders for such an abnormal result. The likelihood of a provider response in the post-implementation period as compared to the pre-implementation period was analyzed using logistic regression. The provider responses were analyzed using logistic regression to control for potential confounders. The likelihood of a provider response to an abnormal laboratory result did not change significantly after implementation of an LRPS. (Odds Ratio 0.90, 95% CI 0.63-1.30, p-value 0.58) However, when providers did respond to an alert, the type of response was different. The proportion of repeat laboratory tests increased. (26/378 vs. 7/278, p-value = 0.02). Although the laboratory result pager altered healthcare provider behavior in the Neonatal Intensive Care Unit, it did not increase the overall likelihood of provider response.

  15. Real causes of apparent abnormal results in heavy ion reactions

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    Mandaglio G.

    2015-01-01

    Full Text Available We study the effect of the static characteristics of nuclei and dynamics of the nucleus-nucleus interaction in the capture stage of reaction, in the competition between quasifission and complete fusion processes, as well as the angular momentum dependence of the competition between fission and evaporation processes along the de-excitation cascade of the compound nucleus. The results calculated for the mass-asymmetric and less mass-asymmetric reactions in the entrance channel are analyzed in order to investigate the role of the dynamical effects on the yields of the evaporation residue nuclei. We also discuss about uncertainties at the extraction of such relevant physical quantities as Γn/Γtot ratio or also excitation functions from the experimental results due to the not always realistic assumptions in the treatment and analysis of the detected events. This procedure can lead to large ambiguity when the complete fusion process is strongly hindered or when the fast fission contribution is large. We emphasize that a refined multiparameter model of the reaction dynamics as well as a more detailed and checked data analysis are strongly needed in heavy-ion collisions.

  16. Mutant laboratory mice with abnormalities in hair follicle morphogenesis, cycling, and/or structure: an update.

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    Nakamura, Motonobu; Schneider, Marlon R; Schmidt-Ullrich, Ruth; Paus, Ralf

    2013-01-01

    Human hair disorders comprise a number of different types of alopecia, atrichia, hypotrichosis, distinct hair shaft disorders as well as hirsutism and hypertrichosis. Their causes vary from genodermatoses (e.g. hypotrichoses) via immunological disorders (e.g. alopecia areata, autoimmune cicatrical alopecias) to hormone-dependent abnormalities (e.g. androgenetic alopecia). A large number of spontaneous mouse mutants and genetically engineered mice develop abnormalities in hair follicle morphogenesis, cycling, and/or hair shaft formation, whose analysis has proven invaluable to define the molecular regulation of hair growth, ranging from hair follicle development, and cycling to hair shaft formation and stem cell biology. Also, the accumulating reports on hair phenotypes of mouse strains provide important pointers to better understand the molecular mechanisms underlying human hair growth disorders. Since numerous new mouse mutants with a hair phenotype have been reported since the publication of our earlier review on this matter a decade ago, we present here an updated, tabulated mini-review. The updated annotated tables list a wide selection of mouse mutants with hair growth abnormalities, classified into four categories: Mutations that affect hair follicle (1) morphogenesis, (2) cycling, (3) structure, and (4) mutations that induce extrafollicular events (for example immune system defects) resulting in secondary hair growth abnormalities. This synthesis is intended to provide a useful source of reference when studying the molecular controls of hair follicle growth and differentiation, and whenever the hair phenotypes of a newly generated mouse mutant need to be compared with existing ones. Copyright © 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

  17. Abnormal ovarian cancer screening test result: women's informational, psychological and practical needs.

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    Ryan, Patricia Y; Graves, Kristi D; Pavlik, Edward J; Andrykowski, Michael A

    2007-01-01

    Considerable effort has been devoted to the identification of cost-effective approaches to screening for ovarian cancer (OC). Transvaginal ultrasound (TVS) is one such screening approach. Approximately 5-7% of routine TVS screening tests yield abnormal results. Some women experience significant distress after receipt of an abnormal TVS screening test. Four focus groups provided in-depth, qualitative data regarding the informational, psychological, and practical needs of women after the receipt of an abnormal TVS result. Through question and content analytic procedures, we identified four themes: anticipation, emotional response, role of the screening technician, and impact of prior cancer experiences. Results provide initial guidance toward development of interventions to promote adaptive responses after receipt of an abnormal cancer screening test result.

  18. Laboratory results of the AOF system testing

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    Kolb, Johann; Madec, Pierre-Yves; Arsenault, Robin; Oberti, Sylvain; Paufique, Jérôme; La Penna, Paolo; Ströbele, Stefan; Donaldson, Robert; Soenke, Christian; Suárez Valles, Marcos; Kiekebusch, Mario; Argomedo, Javier; Le Louarn, Miska; Vernet, Elise; Haguenauer, Pierre; Duhoux, Philippe; Aller-Carpentier, Emmanuel; Valenzuela, Jose Javier; Guerra, Juan Carlos

    2016-07-01

    For two years starting in February 2014, the AO modules GRAAL for HAWK-I and GALACSI for MUSE of the Adaptive Optics Facility project have undergone System Testing at ESO's Headquarters. They offer four different modes: NGS SCAO, LGS GLAO in the IR, LGS GLAO and LTAO in the visible. A detailed characterization of those modes was made possible by the existence of ASSIST, a test bench emulating an adaptive VLT including the Deformable Secondary Mirror, a star simulator and turbulence generator and a VLT focal plane re-imager. This phase aimed at validating all the possible components and loops of the AO modules before installation at the actual VLT that comprises the added complexity of real LGSs, a harsher non-reproducible environment and the adaptive telescope control. In this paper we present some of the major results obtained and challenges encountered during the phase of System Tests, like the preparation of the Acquisition sequence, the testing of the Jitter loop, the performance optimization in GLAO and the offload of low-order modes from the DSM to the telescope (restricted to the M2 hexapod). The System Tests concluded with the successful acceptance, shipping, installation and first commissioning of GRAAL in 2015 as well as the acceptance and shipping of GALACSI, ready for installation and commissioning early 2017.

  19. Result of China National Accreditation for Laboratories Achieving Good Credit

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ With the efforts of the past 10 years, the accreditation system for laboratories, which is complete in range, integrate on function and normative on operation, has been set up in China, and has achieved good reputation home and abroad. The result of laboratories accreditation is widely admitted and used in the international. Since the China national accreditation system for laboratories entering the international multilateral mutual recognition arrangement, the accreditation for laboratories has been playing an increasingly important role in the international trade. The testing result provided by the accreditation laboratories is required by many international-famous enterprises when they purchase in China, and there have been 37 economic systems admitting the result of China national accreditation for laboratories. More and more governmental departments require using accreditation for laboratories in the administrative management and law enforcement.

  20. Drug-Related Hospital Visits and Admissions Associated with Laboratory or Physiologic Abnormalities-A Systematic-Review.

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    Kerry Wilbur

    Full Text Available Countless studies have demonstrated that many emergency-room visits and hospital admissions are drug-related and that a significant proportion of these drug-related visits (DRVs are preventable. It has not been previously studied which DRVs could be prevented through enhanced monitoring of therapy. The objective of the study was to determine the incidence of DRVs attributed to laboratory or physiologic abnormalities. Three authors independently performed comprehensive searches in relevant health care databases using pre-determined search terms. Articles discussing DRV associated with poisoning, substance abuse, or studied among existing in-patient populations were excluded. Study country, year, sample, design, duration, DRV identification method, proportion of DRVs associated with laboratory or physiologic abnormalities and associated medications were extracted. The three authors independently assessed selected relevant articles according to the Strengthening the reporting of observational studies in epidemiology (STROBE as applicable according to the studies' methodology. The initial literature search yielded a total of 1,524 articles of which 30 articles meeting inclusion criteria and reporting sufficient laboratory or physiologic data were included in the overall analysis. Half employed prospective methodologies, which included both chart review and patient interview; however, the overwhelming majority of identified studies assessed only adverse drug reactions (ADRs as a drug-related cause for DRV. The mean (range prevalence of DRVs found in all studies was 15.4% (0.44%-66.7% of which an association with laboratory or physiologic abnormalities could be attributed to a mean (range of 29.4% (4.3%-78.1% of cases. Most laboratory-associated DRVs could be linked to immunosuppressant, antineoplastic, anticoagulant and diabetes therapy, while physiologic-associated DRVs were attributed to cardiovascular therapies and NSAIDs. Significant proportions

  1. Cerebrospinal fluid abnormalities in patients with syphilis: association with clinical and laboratory features.

    Science.gov (United States)

    Marra, Christina M; Maxwell, Clare L; Smith, Stacy L; Lukehart, Sheila A; Rompalo, Anne M; Eaton, Molly; Stoner, Bradley P; Augenbraun, Michael; Barker, David E; Corbett, James J; Zajackowski, Mark; Raines, Charles; Nerad, Judith; Kee, Romina; Barnett, Scott H

    2004-02-01

    To define clinical and laboratory features that identify patients with neurosyphilis. Subjects (n=326) with syphilis but no previous neurosyphilis who met 1993 Centers for Disease Control and Prevention criteria for lumbar puncture underwent standardized history, neurological examination, venipuncture, and lumbar puncture. Neurosyphilis was defined as a cerebrospinal fluid (CSF) white blood cell count >20 cells/ microL or reactive CSF Venereal Disease Research Laboratory (VDRL) test result. Sixty-five subjects (20.1%) had neurosyphilis. Early syphilis increased the odds of neurosyphilis in univariate but not multivariate analyses. In multivariate analyses, serum rapid plasma reagin (RPR) titer > or =1 : 32 increased the odds of neurosyphilis 10.85-fold in human immunodeficiency virus (HIV)-uninfected subjects and 5.98-fold in HIV-infected subjects. A peripheral blood CD4+ T cell count < or =350 cells/ microL conferred 3.10-fold increased odds of neurosyphilis in HIV-infected subjects. Similar results were obtained when neurosyphilis was more stringently defined as a reactive CSF VDRL test result. Serum RPR titer helps predict the likelihood of neurosyphilis. HIV-induced immune impairment may increase the risk of neurosyphilis.

  2. Abnormal computerized dynamic posturography findings in dizzy patients with normal ENG results.

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    Sataloff, Robert T; Hawkshaw, Mary J; Mandel, Heidi; Zwislewski, Amy B; Armour, Jonathan; Mandel, Steven

    2005-04-01

    The complexities of the balance system create difficulties for professionals interested in testing equilibrium function objectively. Traditionally, electronystagmography (ENG) has been used for this purpose, but it provides information on only a limited portion of the equilibrium system. Computerized dynamic posturography (CDP) is less specific than ENG, but it provides more global insight into a patient's ability to maintain equilibrium under more challenging environmental circumstances. CD Palso appears to be valuable in obtaining objective confirmation of an abnormality in some dizzy patients whose ENG findings are normal. Our review of 33 patients with normal ENG results and abnormal CDP findings suggests that posturography is useful for confirming or quantifying a balance abnormality in some patients whose complaints cannot be confirmed by other tests frequently used by otologists.

  3. 1ST-TRIMESTER SCREENING FOR FETAL CHROMOSOMAL-ABNORMALITIES - PRELIMINARY-RESULTS

    NARCIS (Netherlands)

    VANLITH, JMM

    1991-01-01

    We have started a multicentre trial to study the possibilities of first-trimester maternal serum screening for fetal chromosomal abnormalities. Maternal blood samples were obtained before 13 weeks of gestation. We present the preliminary results of the first 950 patients on alpha-fetoprotein (AFP).

  4. What do women think about abnormal smear test results? A qualitative interview study.

    NARCIS (Netherlands)

    Lagro-Janssen, A.L.M.; Schijf, C.P.T.

    2005-01-01

    The aim of the study is a report of Dutch women's views on abnormal cervical smear test results and the consequences thereof. Twenty-seven women with recent PAP III in their history were interviewed in the context of this qualitative study. GPs often do not inform patients beforehand about the ways

  5. Prevalence of renal and hepatobiliary disease, laboratory abnormalities, and potentially toxic medication exposures among persons with COPD

    Directory of Open Access Journals (Sweden)

    Mapel DW

    2013-03-01

    Full Text Available Douglas W Mapel,1 Jenõ P Marton21Lovelace Clinic Foundation, Albuquerque, New Mexico, NM, USA; 2Health Economics and Outcomes Research, Pfizer Inc, New York, NY, USABackground: The purpose of this study was to describe the prevalence of renal and hepatic disease, related laboratory abnormalities, and potentially hepatotoxic and nephrotoxic medication use in a population-based cohort of persons with chronic obstructive pulmonary disease (COPD.Methods: This was a retrospective case-control cohort analysis of COPD patients enrolled in one regional health system for at least 12 months during a 36-month study period (n = 2284. Each COPD patient was matched by age and gender to up to three persons not diagnosed with COPD (n = 5959.Results: The mean age for cases and controls was 70.3 years, and 52.5% were women. The COPD cohort had significantly higher prevalences (cases/100 of acute, chronic, and unspecified renal failure as compared with controls (1.40 versus 0.59, 2.89 versus 0.79, and 1.09 versus 0.44, respectively. Among the cases, 31.3% had at least one renal or urinary tract diagnosis during the study period, as compared with 21.1% of controls. COPD cases also had more gallbladder disease (2.76 versus 1.63 and pancreatic disease (1.40 versus 0.60, but not hepatic disease. COPD patients were more likely to have at least one serum creatinine level (5.1 versus 2.1 or liver aspartate aminotransferase level (4.5 versus 2.7 that was more than twice the upper limit of normal. COPD patients had prescription fills for an average of 17.6 potentially nephrotoxic and 27.4 hepatotoxic drugs during the study period, as compared with 13.6 and 19.9 for the controls (P value for all comparisons < 0.01.Conclusion: COPD patients have a substantially increased prevalence of renal, gallbladder, and pancreatic diseases, as well as abnormal renal and hepatic laboratory values, but not diagnosed liver disease. COPD patients are also more likely to be prescribed

  6. Results of Laboratory Testing of Advanced Power Strips: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Earle, L.; Sparn, B.

    2012-08-01

    This paper describes the results of a laboratory investigation to evaluate the technical performance of advanced power strip (APS) devices when subjected to a range of home entertainment center and home office usage scenarios.

  7. Spectral Karyotyping for identification of constitutional chromosomal abnormalities at a national reference laboratory

    Directory of Open Access Journals (Sweden)

    Anguiano Arturo

    2012-01-01

    Full Text Available Abstract Spectral karyotyping is a diagnostic tool that allows visualization of chromosomes in different colors using the FISH technology and a spectral imaging system. To assess the value of spectral karyotyping analysis for identifying constitutional supernumerary marker chromosomes or derivative chromosomes at a national reference laboratory, we reviewed the results of 179 consecutive clinical samples (31 prenatal and 148 postnatal submitted for spectral karyotyping. Over 90% of the cases were requested to identify either small supernumerary marker chromosomes (sSMCs or chromosomal exchange material detected by G-banded chromosome analysis. We also reviewed clinical indications of those cases with marker chromosomes in which chromosomal origin was identified by spectral karyotyping. Our results showed that spectral karyotyping identified the chromosomal origin of marker chromosomes or the source of derivative chromosomal material in 158 (88% of the 179 clinical cases; the identification rate was slightly higher for postnatal (89% compared to prenatal (84% cases. Cases in which the origin could not be identified had either a small marker chromosome present at a very low level of mosaicism (

  8. Investigation of newborns with abnormal results in a newborn screening program for four lysosomal storage diseases in Brazil

    Directory of Open Access Journals (Sweden)

    Heydy Bravo

    2017-09-01

    Full Text Available Lysosomal storage diseases (LSDs are genetic disorders, clinically heterogeneous, mainly caused by defects in genes encoding lysosomal enzymes that degrade macromolecules. Several LSDs already have specific therapies that may improve clinical outcomes, especially if introduced early in life. With this aim, screening methods have been established and newborn screening (NBS for some LSDs has been developed. Such programs should include additional procedures for the confirmation (or not of the cases that had an abnormal result in the initial screening. We present here the methods and results of the additional investigation performed in four babies with positive initial screening results in a program of NBS for LSDs performed by a private laboratory in over 10,000 newborns in Brazil. The suspicion in these cases was of Mucopolysaccharidosis I - MPS I (in two babies, Pompe disease and Gaucher disease (one baby each. One case of pseudodeficiency for MPS I, 1 carrier for MPS I, 1 case of pseudodeficiency for Pompe disease and 1 carrier for Gaucher disease were identified. This report illustrates the challenges that may be encountered by NBS programs for LSDs, and the need of a comprehensive protocol for the rapid and precise investigation of the babies who have an abnormal screening result.

  9. Influence of diet on the results of laboratory tests

    Directory of Open Access Journals (Sweden)

    Kinga Lis

    2013-12-01

    Full Text Available Blood and urine laboratory tests are necessary to diagnose the state of the patient. These tests are also helpful in the assessment of diet and nutritional status of the organism. It is recommended that both blood and urine for laboratory tests be collected in the morning, from fasting patients after an overnight rest. These conditions are defined as the standard conditions for collection of material for laboratory testing. Before testing, patients should follow their natural diet and avoid physical exertion, night work, long-distance travel, as well as consumption of alcohol and drugs. They should also reduce the consumption of synthetic vitamins and herbal remedies and other dietary supplements. Medications should be limited to those that are absolutely necessary. All of these factors can affect the results of laboratory tests.

  10. Results of nocturnal penile tumescence studies are abnormal in sexually functional diabetic men.

    Science.gov (United States)

    Nofzinger, E A; Reynolds, C F; Jennings, J R; Thase, M E; Frank, E; Yeager, A; Kupfer, D J

    1992-01-01

    Nocturnal penile tumescence (NPT) studies are commonly used in the assessment of sexual dysfunction in diabetic men. While much of the evidence in favor of its use has come from the observation of markedly abnormal NPT in impotent diabetic men, little research has focused on the quality of nocturnal erections in sexually functional diabetics. Ten diabetic men who reported normal daytime sexual function were studied with 4 nights of polysomnography, including NPT assessment. They had significantly diminished NPT profiles when compared with that of an age-matched, nondiabetic, healthy control group. Without controlling for the effect of diabetes on NPT, between 70% and 90% of sexually functional diabetics had NPT profiles in a range that would be classified as indicative of organic sexual dysfunction for a man presenting for evaluation of sexual dysfunction. The finding of NPT abnormalities in a diabetic man should not be taken as evidence for irreversible sexual dysfunction. Rather, the condition of diabetes appears to result in NPT abnormalities, regardless of the adequacy of daytime sexual function.

  11. Magnetic resonance imaging of sacroiliitis in early seronegative spondylarthropathy. Abnormalities correlated to clinical and laboratory findings

    DEFF Research Database (Denmark)

    Puhakka, K B; Jurik, A G; Schiøttz-Christensen, Berit

    2004-01-01

    OBJECTIVE: To compare a new MRI scoring system of the sacroiliac joints (SIJs) in early spondylarthropathy (SpA) with clinical and laboratory parameters. METHODS: Forty-one patients (24 males, 17 females) with a median age of 26 yr and a median duration of inflammatory low back pain of 19 months...

  12. Fibrinogen geneva II: a new congenitally abnormal fibrinogen alpha chain (Gly17Asp) with a review of similar mutations resulting in abnormal knob A.

    Science.gov (United States)

    Casini, Alessandro; De Maistre, Emmanuel; Casini-Stuppi, Virginie; Fontana, Pierre; Neerman-Arbez, Marguerite; de Moerloose, Philippe

    2014-04-01

    Congenital dysfibrinogenemias are characterized by biosynthesis of a structurally abnormal fibrinogen molecule that exhibits reduced functional levels compared with the level of fibrinogen antigen. To date a large number of mutations have been identified in patients with dysfibrinogenemia. Mutations occurring at the thrombin cleavage site (Arg16-Gly17 in the mature alpha-chain) at the amino-terminal end of the fibrinogen alpha chain are a common cause of the disease. These mutations causing abnormal fibrin polymerization are associated with different phenotypes. Here, we report the identification of a novel heterozygous missense mutation of Glycine 17 (Gly17Asp) in a female patient with mild bleeding manifestations, and compare it with other previously reported mutations also resulting in abnormal knob A.

  13. Correlation of bleeding pattern with endometrial histopathologic results in perimenopausal women with abnormal uterine bleeding

    Directory of Open Access Journals (Sweden)

    Zehra Yilmaz

    2015-06-01

    Full Text Available Background: Abnormal Uterine Bleeding (AUB is referred as bleeding outside of normal menstruation pattern and it is the most common gynecological problem for women of all ages. This study was evaluated the correlation of menstrual bleeding patterns and endometrial histopathological findings in perimenopausal women. Methods: This study was done on perimenopausal aged women presented with AUB for the last 6 months at a gynecology clinic of a tertiary medical center. Only the patients with isolated endometrial causes of AUB were selected for study. A total of 313 cases were included in the study. Abnormal bleeding patterns of the patients were recorded and endometrial sampling was performed to all women. AUB was classified as menorrhagia, metrorrhagia, menometrorrhagia, polymenorrhea, intermenstrual bleeding, and histopathological findings were classified as Proliferative Endometrium (PE, Secretory Endometrium (SE, Disordered Proliferative Pattern (DPP, Endometrial Polyp (EP, Chronic Endometritis (CE, Endometrial Hyperplasia (EH, and Endometrial Adenocarcinoma (CA. Results: The most common bleeding pattern was menorrhagia (45.0% and the most common histopathological finding was PE+SE (52.0% in our study. PE+SE and endometrial hyperplasia without atypia were found more common in menorrhagia group. The most histopathological findings were found PE+SE in menometrorrhagia and polymenorrhea group (P 0.05. Conclusions: We concluded that although menometrorrhagia and polymenorrhea were significantly more associated with PE+SE, intermenstrual bleeding was significantly more associated with EP and CE. It is noteworthy that endometrial hyperplasia without atypia is significantly higher in patients with menorrhagia which is the most common abnormal bleeding pattern in perimenopausal aged women. [Int J Reprod Contracept Obstet Gynecol 2015; 4(3.000: 547-550

  14. Helium at White Dwarf Photospheric Conditions: Preliminary Laboratory Results

    Science.gov (United States)

    Schaeuble, M.; Falcon, R. E.; Gomez, T. A.; Winget, D. E.; Montgomery, M. H.; Bailey, J. E.

    2017-03-01

    We present preliminary results of an experimental study exploring helium at photospheric conditions of white dwarf stars. These data were collected at Sandia National Laboratories' Z-machine, the largest x-ray source on earth. Our helium results could have many applications ranging from validating current DB white dwarf model atmospheres to providing accurate He pressure shifts at varying temperatures and densities. In a much broader context, these helium data can be used to guide theoretical developments in new continuum-lowering models for two-electron atoms. We also discuss future applications of our updated experimental design, which enables us to sample a greater range of densities, temperatures, and gas compositions.

  15. Helium at white dwarf photospheric conditions: preliminary laboratory results

    CERN Document Server

    Schaeuble, Marc; Gomez, Thomas A; Winget, Don E; Montgomery, Michael H; Bailey, James E

    2016-01-01

    We present preliminary results of an experimental study exploring helium at photospheric conditions of white dwarf stars. These data were collected at Sandia National Laboratories' Z-machine, the largest x-ray source on earth. Our helium results could have many applications ranging from validating current DB white dwarf model atmospheres to providing accurate He pressure shifts at varying temperatures and densities. In a much broader context, these helium data can be used to guide theoretical developments in new continuum-lowering models for two-electron atoms. We also discuss future applications of our updated experimental design, which enables us to sample a greater range of densities, temperatures, and gas compositions.

  16. Follow-up of abnormal or inadequate test results in the Danish Cervical Cancer Screening Program

    DEFF Research Database (Denmark)

    Kristiansen, Bettina Kjær

    2014-01-01

    Denmark has a higher incidence of cervical cancer than other Nordic countries, although all Danish women (aged 23–65) are screened regularly to identify possible cervical dysplasia or asymptomatic invasive cancer. Annually 40 000 women receives an abnormal or inadequate test result and a follow......-up recommendation. However problems with delayed follow-up may threaten the effectiveness of the Danish Cervical Cancer Screening Program, as 20% of women are delayed and dysplasia potentially can progress into cancer. Delayed follow-up is found in situations where women either consciously or unconsciously postpone...... will be of great importance to the future organisation of cervical and colorectal cancer screening programmes in Denmark, but will also have international interest because of their similar challenges....

  17. Response to an abnormal ovarian cancer-screening test result: test of the social cognitive processing and cognitive social health information processing models.

    Science.gov (United States)

    Andrykowski, Michael A; Pavlik, Edward J

    2011-04-01

    All cancer screening tests produce a proportion of abnormal results requiring follow up. Consequently, the cancer-screening setting is a natural laboratory for examining psychological and behavioural response to a threatening health-related event. This study tested hypotheses derived from the social cognitive processing and cognitive-social health information processing models in trying to understand response to an abnormal ovarian cancer (OC) screening test result. Women (n = 278) receiving an abnormal screening test result a mean of 7 weeks earlier were assessed prior to a repeat screening test intended to clarify their previous abnormal result. Measures of disposition (optimism, informational coping style), social environment (social support and constraint), emotional processing, distress, and benefit finding were obtained. Regression analyses indicated greater distress was associated with greater social constraint and emotional processing and a monitoring coping style in women with a family history of OC. Distress was unrelated to social support. Greater benefit finding was associated with both greater social constraint and support and greater distress. The primacy of social constraint in accounting for both benefit finding and distress was noteworthy and warrants further research on the role of social constraint in adaptation to stressful events.

  18. COMPILATION OF LABORATORY SCALE ALUMINUM WASH AND LEACH REPORT RESULTS

    Energy Technology Data Exchange (ETDEWEB)

    HARRINGTON SJ

    2011-01-06

    This report compiles and analyzes all known wash and caustic leach laboratory studies. As further data is produced, this report will be updated. Included are aluminum mineralogical analysis results as well as a summation of the wash and leach procedures and results. Of the 177 underground storage tanks at Hanford, information was only available for five individual double-shell tanks, forty-one individual single-shell tanks (e.g. thirty-nine 100 series and two 200 series tanks), and twelve grouped tank wastes. Seven of the individual single-shell tank studies provided data for the percent of aluminum removal as a function of time for various caustic concentrations and leaching temperatures. It was determined that in most cases increased leaching temperature, caustic concentration, and leaching time leads to increased dissolution of leachable aluminum solids.

  19. Loss of maintenance DNA methylation results in abnormal DNA origin firing during DNA replication

    Energy Technology Data Exchange (ETDEWEB)

    Haruta, Mayumi [Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan); Shimada, Midori, E-mail: midorism@med.nagoya-cu.ac.jp [Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan); Nishiyama, Atsuya; Johmura, Yoshikazu [Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan); Le Tallec, Benoît; Debatisse, Michelle [Institut Curie, Centre de Recherche, 26 rue d’Ulm, CNRS UMR 3244, 75248 ParisCedex 05 (France); Nakanishi, Makoto, E-mail: mkt-naka@med.nagoya-cu.ac.jp [Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan)

    2016-01-22

    The mammalian maintenance methyltransferase DNMT1 [DNA (cytosine-5-)-methyltransferase 1] mediates the inheritance of the DNA methylation pattern during replication. Previous studies have shown that depletion of DNMT1 causes a severe growth defect and apoptosis in differentiated cells. However, the detailed mechanisms behind this phenomenon remain poorly understood. Here we show that conditional ablation of Dnmt1 in murine embryonic fibroblasts (MEFs) resulted in an aberrant DNA replication program showing an accumulation of late-S phase replication and causing severely defective growth. Furthermore, we found that the catalytic activity and replication focus targeting sequence of DNMT1 are required for a proper DNA replication program. Taken together, our findings suggest that the maintenance of DNA methylation by DNMT1 plays a critical role in proper regulation of DNA replication in mammalian cells. - Highlights: • DNMT1 depletion results in an abnormal DNA replication program. • Aberrant DNA replication is independent of the DNA damage checkpoint in DNMT1cKO. • DNMT1 catalytic activity and RFT domain are required for proper DNA replication. • DNMT1 catalytic activity and RFT domain are required for cell proliferation.

  20. Results from laboratory and field testing of nitrate measuring spectrophotometers

    Science.gov (United States)

    Snazelle, Teri T.

    2015-01-01

    Five ultraviolet (UV) spectrophotometer nitrate analyzers were evaluated by the U.S. Geological Survey (USGS) Hydrologic Instrumentation Facility (HIF) during a two-phase evaluation. In Phase I, the TriOS ProPs (10-millimeter (mm) path length), Hach NITRATAX plus sc (5-mm path length), Satlantic Submersible UV Nitrate Analyzer (SUNA, 10-mm path length), and S::CAN Spectro::lyser (5-mm path length) were evaluated in the HIF Water-Quality Servicing Laboratory to determine the validity of the manufacturer's technical specifications for accuracy, limit of linearity (LOL), drift, and range of operating temperature. Accuracy specifications were met in the TriOS, Hach, and SUNA. The stock calibration of the S::CAN required two offset adjustments before the analyzer met the manufacturer's accuracy specification. Instrument drift was observed only in the S::CAN and was the result of leaching from the optical path insert seals. All tested models, except for the Hach, met their specified LOL in the laboratory testing. The Hach's range was found to be approximately 18 milligrams nitrogen per liter (mg-N/L) and not the manufacturer-specified 25 mg-N/L. Measurements by all of the tested analyzers showed signs of hysteresis in the operating temperature tests. Only the SUNA measurements demonstrated excessive noise and instability in temperatures above 20 degrees Celsius (°C). The SUNA analyzer was returned to the manufacturer at the completion of the Phase II field deployment evaluation for repair and recalibration, and the performance of the sensor improved significantly.

  1. pitx2 Deficiency results in abnormal ocular and craniofacial development in zebrafish.

    Directory of Open Access Journals (Sweden)

    Yi Liu

    Full Text Available Human PITX2 mutations are associated with Axenfeld-Rieger syndrome, an autosomal-dominant developmental disorder that involves ocular anterior segment defects, dental hypoplasia, craniofacial dysmorphism and umbilical abnormalities. Characterization of the PITX2 pathway and identification of the mechanisms underlying the anomalies associated with PITX2 deficiency is important for better understanding of normal development and disease; studies of pitx2 function in animal models can facilitate these analyses. A knockdown of pitx2 in zebrafish was generated using a morpholino that targeted all known alternative transcripts of the pitx2 gene; morphant embryos generated with the pitx2(ex4/5 splicing-blocking oligomer produced abnormal transcripts predicted to encode truncated pitx2 proteins lacking the third (recognition helix of the DNA-binding homeodomain. The morphological phenotype of pitx2(ex4/5 morphants included small head and eyes, jaw abnormalities and pericardial edema; lethality was observed at ∼6-8-dpf. Cartilage staining revealed a reduction in size and an abnormal shape/position of the elements of the mandibular and hyoid pharyngeal arches; the ceratobranchial arches were also decreased in size. Histological and marker analyses of the misshapen eyes of the pitx2(ex4/5 morphants identified anterior segment dysgenesis and disordered hyaloid vasculature. In summary, we demonstrate that pitx2 is essential for proper eye and craniofacial development in zebrafish and, therefore, that PITX2/pitx2 function is conserved in vertebrates.

  2. pitx2 Deficiency results in abnormal ocular and craniofacial development in zebrafish.

    Science.gov (United States)

    Liu, Yi; Semina, Elena V

    2012-01-01

    Human PITX2 mutations are associated with Axenfeld-Rieger syndrome, an autosomal-dominant developmental disorder that involves ocular anterior segment defects, dental hypoplasia, craniofacial dysmorphism and umbilical abnormalities. Characterization of the PITX2 pathway and identification of the mechanisms underlying the anomalies associated with PITX2 deficiency is important for better understanding of normal development and disease; studies of pitx2 function in animal models can facilitate these analyses. A knockdown of pitx2 in zebrafish was generated using a morpholino that targeted all known alternative transcripts of the pitx2 gene; morphant embryos generated with the pitx2(ex4/5) splicing-blocking oligomer produced abnormal transcripts predicted to encode truncated pitx2 proteins lacking the third (recognition) helix of the DNA-binding homeodomain. The morphological phenotype of pitx2(ex4/5) morphants included small head and eyes, jaw abnormalities and pericardial edema; lethality was observed at ∼6-8-dpf. Cartilage staining revealed a reduction in size and an abnormal shape/position of the elements of the mandibular and hyoid pharyngeal arches; the ceratobranchial arches were also decreased in size. Histological and marker analyses of the misshapen eyes of the pitx2(ex4/5) morphants identified anterior segment dysgenesis and disordered hyaloid vasculature. In summary, we demonstrate that pitx2 is essential for proper eye and craniofacial development in zebrafish and, therefore, that PITX2/pitx2 function is conserved in vertebrates.

  3. Maternal vitamin B12 deficiency and abnormal cell-free DNA results in pregnancy

    NARCIS (Netherlands)

    Schuring-Blom, Heleen; Lichtenbelt, Klaske; van Galen, Karin; Elferink, Martin; Weiss, Marjan; Vermeesch, Joris Robert; Page-Christiaens, Lieve

    2016-01-01

    What's Already Known about this Topic? Prenatal testing with cell-free DNA may incidentally identify maternal genetic anomalies and malignancies. What does this Study Add? Profound vitamin B12 deficiency with intramedullary hemolysis may cause abnormal genomic patterns that can be detected by

  4. EM techniques for archaeological laboratory experiments: preliminary results

    Science.gov (United States)

    Capozzoli, Luigi; De Martino, Gregory; Giampaolo, Valeria; Raffaele, Luongo; Perciante, Felice; Rizzo, Enzo

    2015-04-01

    The electromagnetic techniques (EM) are based on the investigation of subsoil geophysical parameters and in the archaeological framework they involve in studying contrasts between the buried cultural structures and the surrounding materials. Unfortunately, the geophysical contrast between archaeological features and surrounding soils sometimes are difficult to define due to problems of sensitivity and resolution both related on the characteristic of the subsoil and the geophysical methods. For this reason an experimental activity has been performed in the Hydrogeosite laboratory addressed on the assessment of the capability of geophysical techniques to detect archeological remains placed in the humid/saturated subsoil. At Hydrogeosite Laboratory of CNR-IMAA, a large scale sand-box is located, consisting on a pool shape structures of 230m3 where archaeological remains have been installed . The remains are relative to a living environment and burial of Roman times (walls, tombs, roads, harbour, etc.) covered by sediments. In order to simulate lacustrine and wetland condition and to simulate extreme events (for example underwater landslide, fast natural erosion coast, etc.) the phreatic level was varied and various acquisitions for the different scenarios were performed. In order to analyze the EM behavior of the buried small archaeological framework, ground penetrating radar (GPR) and electrical resistivity tomographies were performed. With GPR, analysis in time domain and frequency domain were performed and coupled to information obtained through resistivity analysis with the support of numerical simulations used to compare the real data with those modeled. A dense grid was adopted for 400 and 900 MHz e-m acquisitions in both the directions, the maximum depth of investigation was limited and less than 3 meters. The same approach was used for ERT acquisition where different array are employed, in particular 3D configuration was used to carry out a 3D resistivity

  5. Solving the MCAO partial illumination issue and laboratory results

    Science.gov (United States)

    Santhakumari, K. K. R.; Arcidiacono, C.; Bertram, T.; Berwein, J.; Herbst, T. M.; Ragazzoni, R.

    2016-07-01

    Telescopes or instruments equipped with Multi-Conjugate Adaptive Optics (MCAO) provide uniform turbulence correction over a wide Field of View (FoV), thereby overcoming the problems of isoplanatism and enabling previously challenging science. LINC-NIRVANA (LN), the German-Italian near-infrared high-resolution imager for the Large Binocular Telescope (LBT), has an advanced and unique MCAO module, which uses the Optical Co-addition of Layer- Oriented Multiple-FoV Natural Guide Star approach to MCAO with pyramid wavefront sensing. The layer-oriented wavefront correction can be performed by conjugating the Deformable Mirrors (DM) and the respective Wavefront Sensors (WFS) to the corresponding atmospheric layers. LN corrects for the aberrations in two different layers. The ground layer, conjugated to the telescope pupil 100m above LBT, is corrected by the Ground-layer Wavefront Sensors (GWS) driving the LBT adaptive secondary mirrors, and a higher layer 7.1km above the telescope is corrected by the High-layer Wavefront Sensors (HWS) driving a pair of Xinetics DMs on the LN bench. At the ground layer, the footprints of the stars overlap completely and every star footprint illuminates the entire pupil-plane. However, for a higher layer, the footprints do not overlap completely and each star illuminates a different region of the conjugated plane. Lack of stars, therefore, results in some regions in this "meta-pupil"-plane not being illuminated, implying no information regarding the aberrations in these areas. The optimum way of correcting the high layer, given this limited information, is the crux of the "partial illumination issue". In this paper, we propose a solution for this issue and discuss laboratory results from the aligned LN bench in the lab. Currently, LN has completed the re-integration and re-alignment at LBT. In early June 2016, we tested our partial illumination algorithm in the instrument's final configuration in the LBT mountain lab, using simulated stars

  6. Results of Laboratory Testing of Advanced Power Strips

    Energy Technology Data Exchange (ETDEWEB)

    Earle, L. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Sparn, B. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2012-08-01

    Presented at the ACEEE Summer Study on Energy Efficiency in Buildings on August 12-17, 2012, this presentation reports on laboratory tests of 20 currently available advanced power strip products, which reduce wasteful electricity use of miscellaneous electric loads in buildings.

  7. Electric earthquake precursors: from laboratory results to field observations.

    Science.gov (United States)

    Vallianatos, F.; Triantis, D.; Tzanis, A.; Anastasiadis, C.; Stavrakas, I.

    2003-04-01

    The possibility of electrical earthquake precursors (EEP) has long been appreciated, but it has proven difficult to construct a solid theory to describe their generation and expected characteristics, or proven techniques to identify and discriminate true precursors from noise. To this end, a large number of laboratory experiments have been conducted, which have demonstrated the generation of transient electric potential prior to rupture in both dry and wet rock specimens. The principal mechanisms proposed to explain these observations are the piezo-electric and electrokinetic effects. The often quoted in the literature piezoelectric effect, cannot explain why non-piezoelectric rock specimens can also generate precursory electric phenomena. Streaming potential can be generated in many kinds of saturated rocks, but the fracturing of dry rocks also produces transient electric effects. Therefore, these two mechanisms may not be the basic contributors to the precursory phenomena observed in the laboratory (and to possible EEP signals). Herein we present a series of laboratory experiments on the microfracturing electrification of dry marble samples under stress, and discuss their possible relationship to field observations of purported EEP. The marble samples were subjected to constant and variable stress-rate uniaxial compression, in both the elastic and the plastic region. During the experiment, pressure stimulated currents (PSC) were observed, which show a clear dependence on the stress rate and mechanical state of the material (the Young modulus), decreasing in frequency and intensity with increasing damage during repeated loading cycles. Explanation of these observations is attempted with a theoretical model involving microfracturing and the motion of charged edge dislocations (MCD) as the primary electrification mechanism, after Tzanis and Vallianatos (Seismo Electromagnetics: Lithosphere-Atmosphere-Ionosphere Coupling, TERRAPUB, 2002). The PSC waveforms and mode

  8. Analysis of patterns of patient compliance after an abnormal Pap smear result: the influence of demographic characteristics on patient compliance.

    Science.gov (United States)

    Rojas, Christine; Zhou, Ming K; Khamis, Harry J; Amesse, Lawrence

    2013-07-01

    This study aimed to determine population characteristics that correlate to suboptimal follow-up after an abnormal cervical cytology result. Nonpregnant women, ages 21 to 65 years, with newly diagnosed abnormal cervical cytology result between January 2009 and January 2012 at an urban clinic were eligible for inclusion in this retrospective chart review. Cervical cytology data and demographic characteristics such as age, ethnicity, employment, marital and smoking status, health insurance and number of pregnancies were abstracted from electronic medical record. A log-linear model was used to determine which factors influenced patient compliance. Of the total of 206 women, 78 (37.9%) had optimal follow-up and 128 (62.1%) had suboptimal follow-up. The 3 variables that were statistically significant in influencing patient follow-up after adjusted analyses included severity of cytology result (p = .0013), ethnicity (p = .02), and employment status (p = .0159). The risk ratio for optimal follow-up for those with severe cytology result was 1.81; for the non-whites, 1.77; and for the employed, 1.53. Ethnicity, severity of cervical cytology result, and employment status play an important role in patient follow-up after an abnormal cervical cytology result. Detecting trends in our patient population that influence adherence to follow-up will help health care providers formulate strategies that target this problem.

  9. Prevalence of extracranial venous abnormalities: results from a sample of 586 multiple sclerosis patients.

    Science.gov (United States)

    Simka, M; Latacz, P; Ludyga, T; Kazibudzki, M; Swierad, M; Janas, P; Piegza, J

    2011-01-01

    The aim of this study was to assess the prevalence of chronic cerebrospinal venous insufficiency in an unselected cohort of multiple sclerosis (MS) patients. A total of 586 patients with clinically defined MS underwent catheter venography of the internal jugular veins, brachiocephalic veins and azygos vein. The following findings were regarded as pathologic: no outflow, slowed outflow, reversal of flow direction, prestenotic dilation accompanied by impaired outflow, outflow through collaterals, intraluminal structures obstructing the vein, hypoplasia, agenesia or significant narrowing of the vein. Venous abnormalities were found in 563 patients (96.1%). Lesions in one vein were found in 43.5%, in two veins in 49.5%, and in three veins in 3.1% of patients. Venous pathologies in the right internal jugular vein were found in 64.0% of patients, in the left internal jugular vein in 81.7%, in the left brachiocephalic vein in 1.0%, and in the azygos vein in 4.9%. Venous pathologies were found to be highly associated with MS, yet the clinical relevance of this phenomenon remains to be established.

  10. Reduction of mitoferrin results in abnormal development and extended lifespan in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Yaguang Ren

    Full Text Available Iron is essential for organisms. It is mainly utilized in mitochondria for biosynthesis of iron-sulfur clusters, hemes and other cofactors. Mitoferrin 1 and mitoferrin 2, two homologues proteins belonging to the mitochondrial solute carrier family, are required for iron delivery into mitochondria. Mitoferrin 1 is highly expressed in developing erythrocytes which consume a large amount of iron during hemoglobinization. Mitoferrin 2 is ubiquitously expressed, whose functions are less known. Zebrafish with mitoferrin 1 mutation show profound hypochromic anaemia and erythroid maturation arrests, and yeast with defects in MRS3/4, the counterparts of mitoferrin 1/2, has low mitochondrial iron levels and grows poorly by iron depletion. Mitoferrin 1 expression is up-regulated in yeast and mouse models of Fiedreich's ataxia disease and in human cell culture models of Parkinson disease, suggesting its involvement in the pathogenesis of diseases with mitochondrial iron accumulation. In this study we found that reduced mitoferrin levels in C. elegans by RNAi treatment causes pleiotropic phenotypes such as small body size, reduced fecundity, slow movement and increased sensitivity to paraquat. Despite these abnormities, lifespan was increased by 50% to 80% in N2 wild type strain, and in further studies using the RNAi sensitive strain eri-1, more than doubled lifespan was observed. The pathways or mechanisms responsible for the lifespan extension and other phenotypes of mitoferrin RNAi worms are worth further study, which may contribute to our understanding of aging mechanisms and the pathogenesis of iron disorder related diseases.

  11. Reduction of mitoferrin results in abnormal development and extended lifespan in Caenorhabditis elegans.

    Science.gov (United States)

    Ren, Yaguang; Yang, Su; Tan, Guoqiang; Ye, Wei; Liu, Danhui; Qian, Xu; Ding, Zhongying; Zhong, Yuhong; Zhang, Jingrui; Jiang, Dandan; Zhao, Yuhong; Lu, Jianxin

    2012-01-01

    Iron is essential for organisms. It is mainly utilized in mitochondria for biosynthesis of iron-sulfur clusters, hemes and other cofactors. Mitoferrin 1 and mitoferrin 2, two homologues proteins belonging to the mitochondrial solute carrier family, are required for iron delivery into mitochondria. Mitoferrin 1 is highly expressed in developing erythrocytes which consume a large amount of iron during hemoglobinization. Mitoferrin 2 is ubiquitously expressed, whose functions are less known. Zebrafish with mitoferrin 1 mutation show profound hypochromic anaemia and erythroid maturation arrests, and yeast with defects in MRS3/4, the counterparts of mitoferrin 1/2, has low mitochondrial iron levels and grows poorly by iron depletion. Mitoferrin 1 expression is up-regulated in yeast and mouse models of Fiedreich's ataxia disease and in human cell culture models of Parkinson disease, suggesting its involvement in the pathogenesis of diseases with mitochondrial iron accumulation. In this study we found that reduced mitoferrin levels in C. elegans by RNAi treatment causes pleiotropic phenotypes such as small body size, reduced fecundity, slow movement and increased sensitivity to paraquat. Despite these abnormities, lifespan was increased by 50% to 80% in N2 wild type strain, and in further studies using the RNAi sensitive strain eri-1, more than doubled lifespan was observed. The pathways or mechanisms responsible for the lifespan extension and other phenotypes of mitoferrin RNAi worms are worth further study, which may contribute to our understanding of aging mechanisms and the pathogenesis of iron disorder related diseases.

  12. Male infertility in China: laboratory finding for AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China.

    Science.gov (United States)

    Wang, Rui-Xue; Fu, Chao; Yang, Ya-Ping; Han, Rong-Rong; Dong, Yuan; Dai, Ru-Lin; Liu, Rui-Zhi

    2010-07-01

    To investigate the frequencies of AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China. Moreover, to compare the prevalence of these abnormalities with other countries and regions in the world. 305 infertile men were enrolled. A complete semen analysis and reproductive hormones were measured according to standard methods. Multiplex polymerase chain reaction (PCR) amplification using nine specific sequence-tagged sites (STS) were used to detect AZF microdeletions. Karyotype analyses were performed on peripheral blood lymphocytes with standard G-banding. Of the 305 infertile men, 28 (9.2%) had AZF microdeletions and 26 (8.5%) had chromosomal abnormalities. The most frequent microdeletions were in the AZFc+d, followed by AZFc, AZFb+c+d and AZFa. A total of 19 patients (82.6%) had Klinefelter's syndrome (47, XXY) in the azoospermic group. The freqencies of AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China were comparable with infertile men from other countries and regions. However, there was a slightly higher prevalence rate of AZF microdeletions in oligozoospermic patients than reported in previous studies.

  13. Loss of the BRCA1-interacting helicase BRIP1 results in abnormal mammary acinar morphogenesis.

    Directory of Open Access Journals (Sweden)

    Kazuhiro Daino

    Full Text Available BRIP1 is a DNA helicase that directly interacts with the C-terminal BRCT repeat of the breast cancer susceptibility protein BRCA1 and plays an important role in BRCA1-dependent DNA repair and DNA damage-induced checkpoint control. Recent studies implicate BRIP1 as a moderate/low-penetrance breast cancer susceptibility gene. However, the phenotypic effects of BRIP1 dysfunction and its role in breast cancer tumorigenesis remain unclear. To explore the function of BRIP1 in acinar morphogenesis of mammary epithelial cells, we generated BRIP1-knockdown MCF-10A cells by short hairpin RNA (shRNA-mediated RNA interference and examined its effect in a three-dimensional culture model. Genome-wide gene expression profiling by microarray and quantitative RT-PCR were performed to identify alterations in gene expression in BRIP1-knockdown cells compared with control cells. The microarray data were further investigated using the pathway analysis and Gene Set Enrichment Analysis (GSEA for pathway identification. BRIP1 knockdown in non-malignant MCF-10A mammary epithelial cells by RNA interference induced neoplastic-like changes such as abnormal cell adhesion, increased cell proliferation, large and irregular-shaped acini, invasive growth, and defective lumen formation. Differentially expressed genes, including MCAM, COL8A1, WIPF1, RICH2, PCSK5, GAS1, SATB1, and ELF3, in BRIP1-knockdown cells compared with control cells were categorized into several functional groups, such as cell adhesion, polarity, growth, signal transduction, and developmental process. Signaling-pathway analyses showed dysregulation of multiple cellular signaling pathways, involving LPA receptor, Myc, Wnt, PI3K, PTEN as well as DNA damage response, in BRIP1-knockdown cells. Loss of BRIP1 thus disrupts normal mammary morphogenesis and causes neoplastic-like changes, possibly via dysregulating multiple cellular signaling pathways functioning in the normal development of mammary glands.

  14. Mars Science Laboratory: Results From Bradbury Landing to Glenelg

    Science.gov (United States)

    Grotzinger, John; Blake, Dave; Crisp, Joy; Edgett, Ken; Gellert, Ralf; Gomez Elvira, Javier; Hassler, Don; Mahaffy, Paul; Malin, Mike; Mitrofanov, Igor; Meyer, Michael; Vasavada, Ashwin; Wiens, Roger; MSL Science Team

    2013-04-01

    The Mars Science Laboratory rover, Curiosity, analyzed rocks, soils, and the atmosphere between Bradbury Landing and the contact with a light-toned, fractured , high-thermal inertia unit ~500 meters to the east ("Glenelg"). A number of in-place outcrops were encountered along this traverse that allows a simple stratigraphy to be con-structed. A variety of siliciclastic sedimentary rocks are present in the section, possibly also including minor basaltic volcanics. At several localities en route to Glenelg, Curiosity observed conglomeratic bedrock containing rounded pebbles ranging in size from 5-40 mm, forming beds at least 5 cm thick with locally well-developed planar stratification; this, plus grain-supported and imbricated clast fabrics suggest transport in aqueous flows with depths of 0.1-0.8 m, and velocities of 14-63 cm/sec. These conglomerates were likely derived from the Gale crater rim and transported down the Peace Vallis channel network; ChemCam data suggest the presence of feldspar and basaltic composition rock fragments as pebbles. APXS and ChemCam data show the out-of-place rock, "Jake Matijevic", to have an evolved, alkaline composi-tion similar to nepheline-normative muegerites, and suggestive of high pressure partial melting of the mantle. Other, stratigraphically in-place rocks show basanitic composition, with high K2O, low SiO2, and high FeO. Between Sols 56 and 110 Curiosity studied the "Rocknest" eolian deposit which was selected for scooping and eventual delivery to CheMin and SAM. The APXS composition of this deposit is consistent with average Mars soils encountered by previous missions (SO3 + Cl ~6 wt.%). Scooped samples delivered to CheMin reveal the presence of forsterite, pigeonite, augite, plagioclase, and several trace minerals including quartz, anhydrite, magnetite, hematite and illmenite. SAM analysis of the scooped soil yielded four different Evolved Gas Analysis (EGA) experiments depending on the temperature at which evolved gases

  15. Translating laboratory compaction test results to field scale

    NARCIS (Netherlands)

    Roholl, J.A.; Thienen-Visser, K. van; Breunese, J.N.

    2016-01-01

    In recent studies on the surface subsidence caused by hydrocarbon recovery of the Groningen gas field, the predicted subsidence is overestimated if results of compaction experiments are not corrected by an empirical `upscaling factor'. In order to find an explanation for this `upscaling factor', an

  16. Tomography and calibration for Raven: from simulations to laboratory results

    Science.gov (United States)

    Jackson, Kate; Correia, Carlos; Lardière, Olivier; Andersen, Dave; Bradley, Colin; Pham, Laurie; Blain, Célia; Nash, Reston; Gamroth, Darryl; Véran, Jean-Pierre

    2014-07-01

    This paper discusses static and dynamic tomographic wave-front (WF) reconstructors tailored to Multi-Object Adaptive Optics (MOAO) for Raven, the first MOAO science and technology demonstrator recently installed on an 8m telescope. We show the results of a new minimum mean- square error (MMSE) solution based on spatio-angular (SA) correlation functions, which extends previous work in Correia et al, JOSA-A 20131 to adopt a zonal representation of the wave-front and its associated signals. This solution is outlined for the static reconstruction and then extended for the use of stand-alone temporal prediction and as a prediction model in a pupil plane based Linear Quadratic Gaussian (LQG) algorithm. We have fully tested our algorithms in the lab and compared the results to simulations of the Raven system. These simulations have shown that an increase in limiting magnitude of up to one magnitude can be expected when prediction is implemented and up to two magnitudes when the LQG is used.

  17. Mineralogical Results from the Mars Science Laboratory Rover Curiosity

    Science.gov (United States)

    Blake, David Frederick.

    2017-01-01

    NASA's CheMin instrument, the first X-ray Diffractometer flown in space, has been operating on Mars for nearly five years. CheMin was first to establish the quantitative mineralogy of the Mars global soil (1). The instrument was next used to determine the mineralogy of a 3.7 billion year old lacustrine mudstone, a result that, together with findings from other instruments on the MSL Curiosity rover, documented the first habitable environment found on another planet (2). The mineralogy of this mudstone from an ancient playa lake was also used to derive the maximum concentration of CO2 in the early Mars atmosphere, a surprisingly low value that calls into question the current theory that CO2 greenhouse warming was responsible for the warm and wet environment of early Mars. CheMin later identified the mineral tridymite, indicative of silica-rich volcanism, in mudstones of the Murray formation on Mt. Sharp. This discovery challenges the paradigm of Mars as a basaltic planet and ushers in a new chapter of comparative terrestrial planetology (3). CheMin is now being used to systematically sample the sedimentary layers that comprise the lower strata of Mt. Sharp, a 5,000 meter sequence of sedimentary rock laid down in what was once a crater lake, characterizing isochemical sediments that through their changing mineralogy, document the oxidation and drying out of the Mars in early Hesperian time.

  18. Follow-up of abnormal or inadequate test results in the Danish Cervical Cancer Screening Program

    DEFF Research Database (Denmark)

    Kristiansen, Bettina Kjær

    2014-01-01

    -up recommendation. However problems with delayed follow-up may threaten the effectiveness of the Danish Cervical Cancer Screening Program, as 20% of women are delayed and dysplasia potentially can progress into cancer. Delayed follow-up is found in situations where women either consciously or unconsciously postpone...... follow-up, or because of organizational aspects of the screening program, where communication regarding test results can fail either in content or with delay.This study will evaluate two interventions designed to increase follow-up: 1) A letter with the test result and potential recommendation for follow...

  19. Reshaping the laboratory results presentation layer: three interfaces for handheld devices.

    Science.gov (United States)

    Ehrler, Frederic; Brissaud, Marion; Wipfli, Rolf; Lovis, Christian

    2015-01-01

    The interpretation of laboratory results is a critical part of the clinical decision making process. The proper understanding of many clinical conditions depends on the identification of evidences in the laboratory reports. If the classic tabular presentation of laboratory results has demonstrated its efficiency since many years, the increase number of potential results, the increased complexity of cases and the time shortage to analyses cases raise the question of finding more efficient ways of displaying these results to clinicians. The presentation layer becomes even more crucial when it comes to small-sized interactive displays. In this work, we discuss three alternative graphical representations of laboratory results adapted to handheld devices.

  20. [An analysis of laboratory results of parameters of organ function in patients with heat stroke].

    Science.gov (United States)

    Ye, Jun; Mo, Weiming; Chen, Yan; Yang, Aiping

    2015-08-01

    To explore the role of parameters of organ function during heat stroke ( HS ) on the prognosis, and to form the treatment strategy through an analysis of parameters of organ function during HS. A retrospective study was conducted. Thirty-seven patients with HS ( HS group ) and 54 patients with mild-to-moderate stroke ( stroke group ) admitted to Zhejiang Xiaoshan Hospital from 2011 to 2014 were enrolled. The experimental results of organs function indicators for patients were recorded including: (1) cardiac markers: troponin I ( TnI ); (2) myocardium zymogram: creatine kinase ( CK ), MB isoenzyme of creatine kinase ( CK-MB ), lactate dehydrogenase ( LDH ), and aspartate aminotransferase ( AST ); (3) renal function indexes: blood urea nitrogen ( BUN ), uric acid ( UA ), and serum creatinine ( SCr ); (4) electrolyte: serum K(+), Na(+), and Cl(-); (5)coagulation function: prothrombin time ( PT ), international normalized ratio ( INR ), activated partial thromboplastin time ( APTT ), thrombin time ( TT ), fibrinogen ( FIB ), and D-dimer; (6) blood gas analysis: pH value, arterial partial pressure of carbon dioxide ( PaCO(2)), base excess ( BE ), standard bicarbonate ( SB ), and actual bicarbonate ( AB ); (7) routine blood test: blood platelet count ( PLT ); (8) hepatic function: alanine aminotransferase ( ALT ). Abnormal rates of laboratory parameters of 37 HS patients were statistically analyzed. Various laboratory parameters of organs function as well as the initial value and extreme value ( maximum or minimum value ) during treatment of CK and PLT in HS patients were compared between two groups. The abnormal rates of 37 HS patients were more than 70%, including incipient value of TnI, CK, LDH, AST, serum Na(+), ALT, D-dimer, PaCO(2), AB, maximum value of CK, and minimum value of PLT, the abnormal rates being 73.0%, 70.3%, 81.1%, 78.4%, 78.4%, 70.3%, 70.3%, 70.3%, 75.7%, 81.1%, 75.7%, respectively. The abnormal rates of other parameters were less than 70%. There were

  1. Results of an inter and intra laboratory exercise on the assessment of complex autosomal DNA profiles.

    Science.gov (United States)

    Benschop, Corina C G; Connolly, Edward; Ansell, Ricky; Kokshoorn, Bas

    2017-01-01

    The interpretation of complex DNA profiles may differ between laboratories and reporting officers, which can lead to discrepancies in the final reports. In this study, we assessed the intra and inter laboratory variation in DNA mixture interpretation for three European ISO17025-accredited laboratories. To this aim, 26 reporting officers analyzed five sets of DNA profiles. Three main aspects were considered: 1) whether the mixed DNA profiles met the criteria for comparison to a reference profile, 2) the actual result of the comparison between references and DNA profiling data and 3) whether the weight of the DNA evidence could be assessed. Similarity in answers depended mostly on the complexity of the tasks. This study showed less variation within laboratories than between laboratories which could be the result of differences between internal laboratory guidelines and methods and tools available. Results show the profile types for which the three laboratories report differently, which informs indirectly on the complexity threshold the laboratories employ. Largest differences between laboratories were caused by the methods available to assess the weight of the DNA evidence. This exercise aids in training forensic scientists, refining laboratory guidelines and explaining differences between laboratories in court. Undertaking more collaborative exercises in future may stimulate dialog and consensus regarding interpretation. For training purposes, DNA profiles of the mixed stains and questioned references are made available. Copyright © 2016 The Chartered Society of Forensic Sciences. Published by Elsevier Ireland Ltd. All rights reserved.

  2. Genetic ablation of Dicer in adult forebrain neurons results in abnormal tau hyperphosphorylation and neurodegeneration

    DEFF Research Database (Denmark)

    Hébert, Sébastien S; Papadopoulou, Aikaterini S; Smith, Pascal

    2010-01-01

    Type III RNase Dicer is responsible for the maturation and function of microRNA (miRNA) molecules in the cell. It is now well-documented that Dicer and the fine-tuning of the miRNA gene network are important for neuronal integrity. However, the underlying mechanisms involved in neuronal death...... demonstrate that miRNAs belonging to the miR-15 family are potent regulators of ERK1 expression in mouse neuronal cells and co-expressed with ERK1/2 in vivo. Finally, we show that miR-15a is specifically downregulated in Alzheimer's disease brain. In summary, these results support the hypothesis that changes...... in the miRNA network may contribute to a neurodegenerative phenotype by affecting tau phosphorylation....

  3. Loss of maintenance DNA methylation results in abnormal DNA origin firing during DNA replication.

    Science.gov (United States)

    Haruta, Mayumi; Shimada, Midori; Nishiyama, Atsuya; Johmura, Yoshikazu; Le Tallec, Benoît; Debatisse, Michelle; Nakanishi, Makoto

    2016-01-22

    The mammalian maintenance methyltransferase DNMT1 [DNA (cytosine-5-)-methyltransferase 1] mediates the inheritance of the DNA methylation pattern during replication. Previous studies have shown that depletion of DNMT1 causes a severe growth defect and apoptosis in differentiated cells. However, the detailed mechanisms behind this phenomenon remain poorly understood. Here we show that conditional ablation of Dnmt1 in murine embryonic fibroblasts (MEFs) resulted in an aberrant DNA replication program showing an accumulation of late-S phase replication and causing severely defective growth. Furthermore, we found that the catalytic activity and replication focus targeting sequence of DNMT1 are required for a proper DNA replication program. Taken together, our findings suggest that the maintenance of DNA methylation by DNMT1 plays a critical role in proper regulation of DNA replication in mammalian cells.

  4. Aid in the detection of myocardial perfusion abnormality utilizing SPECT atlas and images registration: preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Padua, Rodrigo Donizete Santana de [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Div. de Cardiologia]. E-mail: rodrigo_dsp@hcrp.fmrp.usp.br; Oliveira, Lucas Ferrari de [Universidade Federal de Pelotas (UFPel), RS (Brazil). Inst. de Fisica e Matematica. Dept. de Tecnologia da Informacao; Marques, Paulo Mazzoncini de Azevedo [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Centro de Ciencias das Imagens e Fisica Medica; Groote, Jean-Jacques Georges Soares de [Instituto de Ensino Superior COC, Ribeirao Preto, SP (Brazil). Lab. of Artifical Intelligence and Applications; Castro, Adelson Antonio de [Universidade de Sao Paulo (USP), Ribeirao Preto, SP, (Brazil). Faculdade de Medicina; Ana, Lauro Wichert [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Centro de Ciencias das Imagens e Fisica Medica; Simoes, Marcus Vinicius [Universidade de Sao Paulo (USP), Ribeirao Preto, SP, (Brazil). Faculdade de Medicina. Divisao de Cardiologia

    2008-11-15

    To develop an atlas of myocardial perfusion scintigraphy and evaluating its applicability in computer-aided detection of myocardial perfusion defects in patients with ischemic heart disease. The atlas was created with rest-stress myocardial perfusion scintigraphic images of 20 patients of both genders with low probability of coronary artery disease and considered as normal by two experienced observers. Techniques of image registration and mathematical operations on images were utilized for obtaining template images depicting mean myocardial uptake and standard deviation for each gender and physiological condition. Myocardial perfusion scintigraphy images of one male and one female patient were aligned with the corresponding atlas template image, and voxels with myocardial uptake rates two standard deviations below the mean voxel value of the respective region in the atlas template image were highlighted on the tomographic sections and confirmed as perfusion defects by both observe. The present study demonstrated the creation of an atlas of myocardial perfusion scintigraphy with promising results of this tool as an aid in the detection of myocardial perfusion defects. However, further prospective validation with a more representative sample is recommended. (author)

  5. NKCC1-deficiency results in abnormal proliferation of neural progenitor cells of the lateral ganglionic eminence

    Directory of Open Access Journals (Sweden)

    Ana Cathia Magalhães

    2016-08-01

    Full Text Available The proliferative pool of neural progenitor cells is maintained by exquisitely controlled mechanisms for cell cycle regulation. The Na-K-Cl cotransporter NKCC1 is important for regulating cell volume and the proliferation of different cell types in vitro. NKCC1 is expressed in ventral telencephalon of embryonic brains suggesting a potential role in neural development of this region. The ventral telencephalon is a major source for both interneuron and oligodendrocyte precursor cells. Whether NKCC1 is involved in the proliferation of these cell populations remains unknown. In order to assess this question, we monitored several markers for neural, neuronal, and proliferating cells in wild-type and NKCC1 knockout mouse brains. We found that NKCC1 was expressed in neural progenitor cells from the lateral ganglionic eminence (LGE at E12.5. Mice lacking NKCC1 expression displayed reduced PH3-labeled mitotic cells in the ventricular zone and reduced cell cycle reentry. Accordingly, we found a significant reduction of Sp8-labeled immature interneurons migrating from the dorsal LGE in NKCC1-deficient mice at a later developmental stage. Interestingly, at E14.5, NKCC1 regulated also the formation of Olig2-labeled oligodendrocyte precursor cells. Collectively, these findings show that NKCC1 serves in vivo as a modulator of the cell cycle decision in the developing ventral telencephalon at the early stage of neurogenesis. These results present a novel mechanistic avenue to be considered in the recent proposed involvement of chloride transporters in a number of developmentally related diseases such as epilepsy, autism, and schizophrenia.

  6. Loss of Rab27 function results in abnormal lung epithelium structure in mice.

    Science.gov (United States)

    Bolasco, Giulia; Tracey-White, Dhani C; Tolmachova, Tanya; Thorley, Andrew J; Tetley, Teresa D; Seabra, Miguel C; Hume, Alistair N

    2011-03-01

    Rab27 small GTPases regulate secretion and movement of lysosome-related organelles such as T cell cytolytic granules and platelet-dense granules. Previous studies indicated that Rab27a and Rab27b are expressed in the murine lung suggesting that they regulate secretory processes in the lung. Consistent with those studies, we found that Rab27a and Rab27b are expressed in cell types that contain secretory granules: alveolar epithelial type II (AEII) and Clara cells. We then used Rab27a/Rab27b double knockout (DKO) mice to examine the functional consequence of loss of Rab27 proteins in the murine lung. Light and electron microscopy revealed a number of morphological changes in lungs from DKO mice when compared with those in control animals. In aged DKO mice we observed atrophy of the bronchiolar and alveolar epithelium with reduction of cells numbers, thinning of the bronchiolar epithelium and alveolar walls, and enlargement of alveolar airspaces. In these samples we also observed increased numbers of activated foamy alveolar macrophages and granulocyte containing infiltrates together with reduction in the numbers of Clara cells and AEII cells compared with control. At the ultrastructural level we observed accumulation of cytoplasmic membranes and vesicles in Clara cells. Meanwhile, AEII cells in DKO accumulated large mature lamellar bodies and lacked immature/precursor lamellar bodies. We hypothesize that the morphological changes observed at the ultrastructural level in DKO samples result from secretory defects in AEII and Clara cells and that over time these defects lead to atrophy of the epithelium.

  7. Loss of PiT-1 results in abnormal endocytosis in the yolk sac visceral endoderm.

    Science.gov (United States)

    Wallingford, Mary C; Giachelli, Cecilia M

    2014-08-01

    PiT-1 protein is a transmembrane sodium-dependent phosphate (Pi) transporter. PiT-1 knock out (KO) embryos die from largely unknown causes by embryonic day (E) 12.5. We tested the hypothesis that PiT-1 is required for endocytosis in the embryonic yolk sac (YS) visceral endoderm (VE). Here we present data supporting that PiT-1 KO results in a YS remodeling defect and decreased endocytosis in the YS VE. The remodeling defect is not due to an upstream cardiomyocyte requirement for PiT-1, as SM22αCre-specific KO of PiT-1 in the developing heart and the YS mesodermal layer (ME) does not recapitulate the PiT-1 global KO phenotype. Furthermore, we find that high levels of PiT-1 protein localize to the YS VE apical membrane. Together these data support that PiT-1 is likely required in YS VE. During normal development maternal immunoglobulin (IgG) is endocytosed into YS VE and accumulates in the apical side of the VE in a specialized lysosome termed the apical vacuole (AV). We have identified a reduction in PiT-1 KO VE cell height and a striking loss of IgG accumulation in the PiT-1 KO VE. The endocytosis genes Tfeb, Lamtor2 and Snx2 are increased at the RNA level. Lysotracker Red staining reveals a loss of distinct AVs, and yolk sacs incubated ex vivo with phRODO Green Dextran for Endocytosis demonstrate a functional loss of endocytosis. As yolk sac endocytosis is controlled in part by microautophagy, but expression of LC3 had not been examined, we investigated LC3 expression during yolk sac development and found stage-specific LC3 RNA expression that is predominantly from the YS VE layer at E9.5. Normalized LC3-II protein levels are decreased in the PiT-1 KO YS, supporting a requirement for PiT-1 in autophagy in the YS. Therefore, we propose the novel idea that PiT-1 is central to the regulation of endocytosis and autophagy in the YS VE.

  8. 49 CFR 40.183 - What information do laboratories report to MROs regarding split specimen results?

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false What information do laboratories report to MROs regarding split specimen results? 40.183 Section 40.183 Transportation Office of the Secretary of... § 40.183 What information do laboratories report to MROs regarding split specimen results? (a) As...

  9. 48 CFR 1511.011-80 - Data standards for the transmission of laboratory measurement results.

    Science.gov (United States)

    2010-10-01

    ... standards for the transmission of laboratory measurement results. The contracting officer shall insert the... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Data standards for the transmission of laboratory measurement results. 1511.011-80 Section 1511.011-80 Federal Acquisition...

  10. Risk Stratification using Human Papillomavirus Testing among Women with Equivocally Abnormal Cytology: Results from a State-wide Surveillance Program

    Science.gov (United States)

    Gage, Julia C; Hunt, William C; Schiffman, Mark; Katki, Hormuzd A; Cheung, Li C; Cuzick, Jack; Myers, Orrin; Castle, Philip E; Wheeler, Cosette M.

    2016-01-01

    Background Clinical guidelines for cervical cancer screening have incorporated comparative risks of cervical intraepithelial neoplasia grade 3 or cancer (CIN3+) for various screening outcomes to determine management. Few cohorts are large enough to distinguish CIN3+ risks among women with minor abnormalities vs. negative cytology because of low incidence. The New Mexico HPV Pap Registry offers a unique opportunity to evaluate cervical cancer screening in a diverse population across a broad-spectrum of health service delivery. Methods Kaplan-Meier and logistic-Weibull survival models were used to estimate cumulative risks of CIN3+ among women aged 21–64 who were screened in New Mexico between 2007–2011 with negative, equivocal or mildly abnormal cytology, i.e., atypical squamous cells of undetermined significance (ASC-US; with or without HPV triage), or low-grade squamous intraepithelial lesions. Results We identified 452,045 women meeting the selection criteria. The 3-year CIN3+ risks for women with negative, ASC-US and LSIL cytology were: 0.30%, 2.6%, and 5.2%, respectively. HPV triage of ASC-US stratified 3-year CIN3+ risks were 0.72% for HPV-negative and 7.7% for HPV-positive. Risks tended to decline after age 30 for all screening results. Conclusions In this state-wide population-based cohort, cytology and HPV triage of ASC-US stratified women’s CIN3+ risk into similar patterns observed previously, suggesting the validity of screening guidelines for diverse populations in the United States (U.S.). Absolute risk estimates should be compared across other large populations. Impact Strategies for HPV triage of ASC-US derived from clinical trials are upheld in large clinical practice settings and across diverse screening populations in the U.S.. PMID:26518316

  11. Infectious titres of sheep scrapie and bovine spongiform encephalopathy agents cannot be accurately predicted from quantitative laboratory test results.

    Science.gov (United States)

    González, Lorenzo; Thorne, Leigh; Jeffrey, Martin; Martin, Stuart; Spiropoulos, John; Beck, Katy E; Lockey, Richard W; Vickery, Christopher M; Holder, Thomas; Terry, Linda

    2012-11-01

    It is widely accepted that abnormal forms of the prion protein (PrP) are the best surrogate marker for the infectious agent of prion diseases and, in practice, the detection of such disease-associated (PrP(d)) and/or protease-resistant (PrP(res)) forms of PrP is the cornerstone of diagnosis and surveillance of the transmissible spongiform encephalopathies (TSEs). Nevertheless, some studies question the consistent association between infectivity and abnormal PrP detection. To address this discrepancy, 11 brain samples of sheep affected with natural scrapie or experimental bovine spongiform encephalopathy were selected on the basis of the magnitude and predominant types of PrP(d) accumulation, as shown by immunohistochemical (IHC) examination; contra-lateral hemi-brain samples were inoculated at three different dilutions into transgenic mice overexpressing ovine PrP and were also subjected to quantitative analysis by three biochemical tests (BCTs). Six samples gave 'low' infectious titres (10⁶·⁵ to 10⁶·⁷ LD₅₀ g⁻¹) and five gave 'high titres' (10⁸·¹ to ≥ 10⁸·⁷ LD₅₀ g⁻¹) and, with the exception of the Western blot analysis, those two groups tended to correspond with samples with lower PrP(d)/PrP(res) results by IHC/BCTs. However, no statistical association could be confirmed due to high individual sample variability. It is concluded that although detection of abnormal forms of PrP by laboratory methods remains useful to confirm TSE infection, infectivity titres cannot be predicted from quantitative test results, at least for the TSE sources and host PRNP genotypes used in this study. Furthermore, the near inverse correlation between infectious titres and Western blot results (high protease pre-treatment) argues for a dissociation between infectivity and PrP(res).

  12. Prevalence and prognostic significance of ECG abnormalities in HIV-infected patients: results from the Strategies for Management of Antiretroviral Therapy study

    DEFF Research Database (Denmark)

    Soliman, Elsayed Z; Prineas, Ronald J; Roediger, Mollie P

    2011-01-01

    BACKGROUND: It remains debated whether to include resting electrocardiogram (ECG) in the routine care of human immunodeficiency virus (HIV)-infected patients. METHODS: This analysis included 4518 HIV-infected patients (28% women and 29% blacks) from the Strategies for Management of Antiretroviral...... Therapy study, a clinical trial aimed to compare 2 HIV treatment strategies. ECG abnormalities were classified using the Minnesota Code. Cox proportional hazards analysis was used to examine the association between baseline ECG abnormalities and incident cardiovascular disease (CVD). RESULTS: More than...... half of the participants (n = 2325, or 51.5%) had either minor or major ECG abnormalities. Minor ECG abnormalities (48.6%) were more common than major ECG abnormalities (7.7%). During a median follow-up of 28.7 months, 155 participants (3.4%) developed incident CVD. After adjusting for the study...

  13. 48 CFR 1552.211-80 - Data standards for the transmission of laboratory measurement results.

    Science.gov (United States)

    2010-10-01

    ... Texts of Provisions and Clauses 1552.211-80 Data standards for the transmission of laboratory... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Data standards for the transmission of laboratory measurement results. 1552.211-80 Section 1552.211-80 Federal Acquisition...

  14. Gender, Race, and Diet Affect Platelet Function Tests in Normal Subjects Contributing to a High Rate of Abnormal Results

    Science.gov (United States)

    Miller, Connie H.; Rice, Anne S.; Garrett, Katherine; Stein, Sidney F.

    2015-01-01

    Summary To assess sources of variability in platelet function tests in normal subjects, 64 healthy young adults were tested on 2–6 occasions at 2 week intervals using 4 methods: platelet aggregation (AGG) in platelet-rich plasma (PRP) in the Bio/Data PAP-4 Aggregometer (BD) and Chrono-Log Lumi-Aggregometer (CL); and AGG in whole blood (WB) in the CL and Multiplate Platelet Function Analyzer (MP), with ATP release (REL) in CL-PRP and CL-WB. Food and medication exposures were recorded prospectively for 2 weeks prior to each blood draw. At least one AGG abnormality was seen in 21% of 81 drug-free specimens with CL-PRP, 15% with CL-WB, 13% with BD-PRP, and 6% with MP-WB, increasing with inclusion of REL to 28% for CL-PRP and 30% for CL-WB. Epinephrine AGG and REL were significantly reduced in males (P<0.0001). Ristocetin AGG and collagen and thrombin REL were significantly reduced in Blacks (P<0.0001). One-third of specimens drawn following flavonoid-rich food exposures had aberrant results, compared to 8.5% of specimens without such exposures (P=0.0035). PRP tests had less intra-individual variation than WB tests. Gender, race, diet, and test system affected results of platelet function testing in healthy subjects, suggesting caution when interpreting the results of platelet function testing in patients. PMID:24617520

  15. Compendium of Test Results of Recent Single Event Effect Tests Conducted by the Jet Propulsion Laboratory

    Science.gov (United States)

    McClure, Steven S.; Allen, Gregory R.; Irom, Farokh; Scheick, Leif Z.; Adell, Philippe C.; Miyahira, Tetsuo F.

    2010-01-01

    This paper reports heavy ion and proton-induced single event effect (SEE) results from recent tests for a variety of microelectronic devices. The compendium covers devices tested over the last two years by the Jet Propulsion Laboratory.

  16. A mutation in NFkB interacting protein 1 results in cardiomyopathy and abnormal skin development in wa3 mice.

    Science.gov (United States)

    Herron, Bruce J; Rao, Cherie; Liu, Shanming; Laprade, Lisa; Richardson, James A; Olivieri, Emily; Semsarian, Chris; Millar, Sarah E; Stubbs, Lisa; Beier, David R

    2005-03-01

    We have identified waved 3 (wa3), a novel recessive mutation that causes abnormalities of the heart and skin. The cardiac defect results in a severe and rapidly progressive dilated cardiomyopathy. We identified the gene mutated in these mice, which we call NFkB interacting protein1 (Nkip1), using positional cloning. Nkip1 is expressed in skin, heart and vascular endothelium and shares homology with a small family of proteins that play a role in the regulation of transcription factors. A C-terminal fragment of this protein was previously identified as the RelA associated inhibitor (RAI). We show that the full-length protein is larger than previously described, and we confirm that it interacts with NFkB in vivo. Expression analysis of genes known to be regulated by NFkB revealed that Intercellular adhesion molecule 1 (Icam1) expression is consistently elevated in mutant mice. This result suggests that wa3 mutant mice represent a potentially important model for the analysis of the role of inflammatory processes in heart disease.

  17. Factors predictive of abnormal results for computed tomography of the head in horses affected by neurologic disorders: 57 cases (2001-2007).

    Science.gov (United States)

    Sogaro-Robinson, Cristina; Lacombe, Véronique A; Reed, Stephen M; Balkrishnan, Rajesh

    2009-07-15

    To determine neurologic indications associated with abnormal results for computed tomography (CT) imaging of the head of horses affected by neurologic disorders. Retrospective case series. 57 horses. Signalment, history, clinical abnormalities, and clinicopathologic findings were obtained from medical records of horses examined because of neurologic disorders, and precontrast and postcontrast CT images of the head were reviewed. Data were analyzed by use of univariate and multivariate logistic regression. For a horse with abnormal mentation, odds of having abnormal results for CT imaging of the head was 30 times (95% confidence interval [CI], 2.36 to 374.63) the odds for a similar horse without abnormal mentation. For a horse with cranial nerve deficits, odds of having abnormal results for CT imaging of the head was 11 times (95% CI, 1.00 to 127.96) the odds for a similar horse without cranial nerve deficits. For a horse with seizure-like activity, odds of having abnormal results for CT imaging of the head was 0.05 times (95% CI, 0 to 0.90) the odds for a similar horse without seizures. These results suggested that alterations in consciousness and cranial nerve deficits were strong predictors of abnormal CT findings for the head of affected horses. Thus, CT can be a useful complementary diagnostic test in horses with these neurologic deficits. In contrast, alternative diagnostic tests (eg, electroencephalography and magnetic resonance imaging) should be considered in horses with seizure-like activity that do not have head trauma or cranial nerve deficits.

  18. Comparison of pencil-type ionization chamber calibration results and methods between dosimetry laboratories.

    Science.gov (United States)

    Hourdakis, Costas J; Büermann, Ludwig; Ciraj-Bjelac, Olivera; Csete, Istvan; Delis, Harry; Gomola, Igor; Persson, Linda; Novak, Leos; Petkov, Ivailo; Toroi, Paula

    2016-01-01

    A comparison of calibration results and procedures in terms of air kerma length product, PKL, and air kerma, K, was conducted between eight dosimetry laboratories. A pencil-type ionization chamber (IC), generally used for computed tomography dose measurements, was calibrated according to three calibration methods, while its residual signal and other characteristics (sensitivity profile, active length) were assessed. The results showed that the "partial irradiation method" is the preferred method for the pencil-type IC calibration in terms of PKL and it could be applied by the calibration laboratories successfully. Most of the participating laboratories achieved high level of agreement (>99%) for both dosimetry quantities (PKL and K). Estimated relative standard uncertainties of comparison results vary among laboratories from 0.34% to 2.32% depending on the quantity, beam quality and calibration method applied. Detailed analysis of the assigned uncertainties have been presented and discussed.

  19. Towards harmonisation of critical laboratory result management - review of the literature and survey of australasian practices.

    Science.gov (United States)

    Campbell, Ca; Horvath, Ar

    2012-11-01

    Timely release and communication of critical test results may have significant impact on medical decisions and subsequent patient outcomes. Laboratories therefore have an important responsibility and contribution to patient safety. Certification, accreditation and regulatory bodies also require that laboratories follow procedures to ensure patient safety, but there is limited guidance on best practices. In Australasia, no specific requirements exist in this area and critical result reporting practices have been demonstrated to be heterogeneous worldwide.Recognising the need for agreed standards and critical limits, the AACB started a quality initiative to harmonise critical result management throughout Australasia. The first step toward harmonisation is to understand current laboratory practices. Fifty eight Australasian laboratories responded to a survey and 36 laboratories shared their critical limits. Findings from this survey are compared to international practices reviewed in various surveys conducted elsewhere. For the successful operation of a critical result management system, critical tests and critical limits must be defined in collaboration with clinicians. Reporting procedures must include how critical results are identified; who can report and who can receive critical results; what is an acceptable timeframe within which results must be delivered or, if reporting fails, what escalation procedures should follow; what communication channels or systems should be used; what should be recorded and how; and how critical result procedures should be maintained and evaluated to assess impact on outcomes.In this paper we review the literature of current standards and recommendations for critical result management. Key elements of critical result reporting are discussed in view of the findings of various national surveys on existing laboratory practices, including data from our own survey in Australasia. Best practice recommendations are made that laboratories

  20. Psychosocial impact of alternative management policies for low-grade cervical abnormalities: results from the TOMBOLA randomised controlled trial.

    Directory of Open Access Journals (Sweden)

    Linda Sharp

    Full Text Available BACKGROUND: Large numbers of women who participate in cervical screening require follow-up for minor cytological abnormalities. Little is known about the psychological consequences of alternative management policies for these women. We compared, over 30-months, psychosocial outcomes of two policies: cytological surveillance (repeat cervical cytology tests in primary care and a hospital-based colposcopy examination. METHODS: Women attending for a routine cytology test within the UK NHS Cervical Screening Programmes were eligible to participate. 3399 women, aged 20-59 years, with low-grade abnormal cytology, were randomised to cytological surveillance (six-monthly tests; n = 1703 or initial colposcopy with biopsies and/or subsequent treatment based on colposcopic and histological findings (n = 1696. At 12, 18, 24 and 30-months post-recruitment, women completed the Hospital Anxiety and Depression Scale (HADS. A subgroup (n = 2354 completed the Impact of Event Scale (IES six weeks after the colposcopy episode or first surveillance cytology test. Primary outcomes were percentages over the entire follow-up period of significant depression (≥ 8 and significant anxiety (≥ 11; "30-month percentages". Secondary outcomes were point prevalences of significant depression, significant anxiety and procedure-related distress (≥ 9. Outcomes were compared between arms by calculating fully-adjusted odds ratios (ORs for initial colposcopy versus cytological surveillance. RESULTS: There was no significant difference in 30-month percentages of significant depression (OR = 0.99, 95% CI 0.80-1.21 or anxiety (OR = 0.97, 95% CI 0.81-1.16 between arms. At the six-week assessment, anxiety and distress, but not depression, were significantly less common in the initial colposcopy arm (anxiety: 7.9% vs 13.4%; OR = 0.55, 95% CI 0.38-0.81; distress: 30.6% vs 39.3%, OR = 0.67 95% CI 0.54-0.84. Neither anxiety nor depression differed between arms at subsequent time

  1. A gain-of-function mutation in the sodium channel gene Scn2a results in seizures and behavioral abnormalities.

    Science.gov (United States)

    Kearney, J A; Plummer, N W; Smith, M R; Kapur, J; Cummins, T R; Waxman, S G; Goldin, A L; Meisler, M H

    2001-01-01

    The GAL879-881QQQ mutation in the cytoplasmic S4-S5 linker of domain 2 of the rat brain IIA sodium channel (Na(v)1.2) results in slowed inactivation and increased persistent current when expressed in Xenopus oocytes. The neuron-specific enolase promoter was used to direct in vivo expression of the mutated channel in transgenic mice. Three transgenic lines exhibited seizures, and line Q54 was characterized in detail. The seizures in these mice began at two months of age and were accompanied by behavioral arrest and stereotyped repetitive behaviors. Continuous electroencephalogram monitoring detected focal seizure activity in the hippocampus, which in some instances generalized to involve the cortex. Hippocampal CA1 neurons isolated from presymptomatic Q54 mice exhibited increased persistent sodium current which may underlie hyperexcitability in the hippocampus. During the progression of the disorder there was extensive cell loss and gliosis within the hippocampus in areas CA1, CA2, CA3 and the hilus. The lifespan of Q54 mice was shortened and only 25% of the mice survived beyond six months of age. Four independent transgenic lines expressing the wild-type sodium channel were examined and did not exhibit any abnormalities. The transgenic Q54 mice provide a genetic model that will be useful for testing the effect of pharmacological intervention on progression of seizures caused by sodium channel dysfunction. The human ortholog, SCN2A, is a candidate gene for seizure disorders mapped to chromosome 2q22-24.

  2. Analytical performances of food microbiology laboratories - critical analysis of 7 years of proficiency testing results.

    Science.gov (United States)

    Abdel Massih, M; Planchon, V; Polet, M; Dierick, K; Mahillon, J

    2016-02-01

    Based on the results of 19 food microbiology proficiency testing (PT) schemes, this study aimed to assess the laboratory performances, to highlight the main sources of unsatisfactory analytical results and to suggest areas of improvement. The 2009-2015 results of REQUASUD and IPH PT, involving a total of 48 laboratories, were analysed. On average, the laboratories failed to detect or enumerate foodborne pathogens in 3·0% of the tests. Thanks to a close collaboration with the PT participants, the causes of outliers could be identified in 74% of the cases. The main causes of erroneous PT results were either pre-analytical (handling of the samples, timing of analysis), analytical (unsuitable methods, confusion of samples, errors in colony counting or confirmation) or postanalytical mistakes (calculation and encoding of results). PT schemes are a privileged observation post to highlight analytical problems, which would otherwise remain unnoticed. In this perspective, this comprehensive study of PT results provides insight into the sources of systematic errors encountered during the analyses. This study draws the attention of the laboratories to the main causes of analytical errors and suggests practical solutions to avoid them, in an educational purpose. The observations support the hypothesis that regular participation to PT, when followed by feed-back and appropriate corrective actions, can play a key role in quality improvement and provide more confidence in the laboratory testing results. © 2015 The Society for Applied Microbiology.

  3. Variability of Creatinine Measurements in Clinical Laboratories: Results from the CRIC Study

    Science.gov (United States)

    Joffe, Marshall; Hsu, Chi-yuan; Feldman, Harold I.; Weir, Matthew; Landis, J.R.; Hamm, L. Lee

    2010-01-01

    Objectives Estimating equations using serum creatinine (SCr) are often used to assess glomerular filtration rate (GFR). Such creatinine (Cr)-based formulae may produce biased estimates of GFR when using Cr measurements that have not been calibrated to reference laboratories. In this paper, we sought to examine the degree of this variation in Cr assays in several laboratories associated with academic medical centers affiliated with the Chronic Renal Insufficiency Cohort (CRIC) Study; to consider how best to correct for this variation, and to quantify the impact of such corrections on eligibility for participation in CRIC. Variability of Cr is of particular concern in the conduct of CRIC, a large multicenter study of subjects with chronic renal disease, because eligibility for the study depends on Cr-based assessment of GFR. Methods A library of 5 large volume plasma specimens from apheresis patients was assembled, representing levels of plasma Cr from 0.8 to 2.4 mg/dl. Samples from this library were used for measurement of Cr at each of the 14 CRIC laboratories repetitively over time. We used graphical displays and linear regression methods to examine the variability in Cr, and used linear regression to develop calibration equations. We also examined the impact of the various calibration equations on the proportion of subjects screened as potential participants who were actually eligible for the study. Results There was substantial variability in Cr assays across laboratories and over time. We developed calibration equations for each laboratory; these equations varied substantially among laboratories and somewhat over time in some laboratories. The laboratory site contributed the most to variability (51% of the variance unexplained by the specimen) and variation with time accounted for another 15%. In some laboratories, calibration equations resulted in differences in eligibility for CRIC of as much as 20%. Conclusions The substantial variability in SCr assays

  4. EEG Abnormalities Are Associated With Poorer Depressive Symptom Outcomes With Escitalopram and Venlafaxine-XR, but Not Sertraline: Results From the Multicenter Randomized iSPOT-D Study.

    Science.gov (United States)

    Arns, Martijn; Gordon, Evian; Boutros, Nash N

    2017-01-01

    Rationale Limited research is available on electrophysiological abnormalities such as epileptiform EEG or EEG slowing in depression and its association with antidepressant treatment response. Objectives We investigated the association between EEG abnormalities and antidepressant treatment response in the international Study to Predict Optimized Treatment in Depression (iSPOT-D). Methods Of 1008 participants with major depressive disorder randomized to escitalopram, sertraline, or venlafaxine-XR, 622 completed 8 weeks of treatment per protocol. The study also recruited 336 healthy controls. Treatment response was established after 8 weeks using the 17-item Hamilton Rating Scale for Depression (HRSD17). The resting-state EEG was assessed at baseline with eyes closed. EEG abnormalities including epileptiform activity, EEG slowing, and alpha peak frequency (APF) were scored for all subjects, blind to treatment outcome. Results Patients and controls did not differ in the occurrence of EEG abnormalities. Furthermore, in the per protocol sample the occurrence of epileptiform EEG and EEG slowing (as a combined marker) were associated with a reduced likelihood of responding to escitalopram (P = .019; odds ratio [OR] = 3.56) and venlafaxine-XR (P = .043; OR = 2.76), but not sertraline (OR = 0.73). The response rates for this "any EEG abnormality" groups versus the "no-abnormality" group were 33% and 64% for escitalopram and 41% and 66% for venlafaxine-XR, respectively. A slow APF was associated with treatment response only in the sertraline group (P = .21; d = .027). Conclusions EEG abnormalities are associated with nonresponse to escitalopram and venlafaxine-XR, but not sertraline, whereas a slow APF is associated to response for sertraline only.

  5. Expressing hNF-LE397K results in abnormal gaiting in a transgenic model of CMT2E

    Science.gov (United States)

    Dale, Jeffrey M.; Villalon, Eric; Shannon, Stephen G.; Barry, Devin M.; Markey, Rachel M.; Garcia, Virginia B.; Garcia, Michael L.

    2012-01-01

    Charcot-Marie-Tooth disease (CMT) is the most commonly inherited peripheral neuropathy. CMT disease signs include distal limb neuropathy, abnormal gaiting, exacerbation of neuropathy, sensory defects, and deafness. We generated a novel line of CMT2E mice expressing a hNF-LE397K transgene, which displayed muscle atrophy of the lower limbs without denervation, proximal reduction in large caliber axons, and decreased nerve conduction velocity. In this study, we demonstrated that hNF-LE397K mice developed abnormal gait of the hind limbs. The identification of severe gaiting defects in combination with previously observed muscle atrophy, reduced axon caliber, and decreased nerve conduction velocity suggests that hNF-LE397K mice recapitulate many of clinical signs associated with CMT2E. Therefore, hNF-LE397K mice provide a context for potential therapeutic intervention. PMID:22288874

  6. Laboratory test result interpretation for primary care doctors in South Africa.

    Science.gov (United States)

    Vanker, Naadira; Faull, Norman H B

    2017-01-01

    Challenges and uncertainties with test result interpretation can lead to diagnostic errors. Primary care doctors are at a higher risk than specialists of making these errors, due to the range in complexity and severity of conditions that they encounter. This study aimed to investigate the challenges that primary care doctors face with test result interpretation, and to identify potential countermeasures to address these. A survey was sent out to 7800 primary care doctors in South Africa. Questionnaire themes included doctors' uncertainty with interpreting test results, mechanisms used to overcome this uncertainty, challenges with appropriate result interpretation, and perceived solutions for interpreting results. Of the 552 responses received, the prevalence of challenges with result interpretation was estimated in an average of 17% of diagnostic encounters. The most commonly-reported challenges were not receiving test results in a timely manner (51% of respondents) and previous results not being easily available (37%). When faced with diagnostic uncertainty, 84% of respondents would either follow-up and reassess the patient or discuss the case with a specialist, and 67% would contact a laboratory professional. The most useful test utilisation enablers were found to be: interpretive comments (78% of respondents), published guidelines (74%), and a dedicated laboratory phone line (72%). Primary care doctors acknowledge uncertainty with test result interpretation. Potential countermeasures include the addition of patient-specific interpretive comments, the availability of guidelines or algorithms, and a dedicated laboratory phone line. The benefit of enhanced test result interpretation would reduce diagnostic error rates.

  7. Auditory localisation of conventional and electric cars : laboratory results and implications for cycling safety

    NARCIS (Netherlands)

    Stelling-Konczak, A. Hagenzieker, M.P. Commandeur, J.J.F. Agterberg, M.J.H. & Wee, B. van

    2016-01-01

    When driven at low speeds, cars operating in electric mode have been found to be quieter than conventional cars. As a result, the auditory cues which pedestrians and cyclists use to assess the presence, proximity and location oncoming traffic may be reduced, posing a safety hazard. This laboratory s

  8. Auditory localisation of conventional and electric cars : laboratory results and implications for cycling safety.

    NARCIS (Netherlands)

    Stelling-Konczak, A. Hagenzieker, M.P. Commandeur, J.J.F. Agterberg, M.J.H. & Wee, B. van

    2016-01-01

    When driven at low speeds, cars operating in electric mode have been found to be quieter than conventional cars. As a result, the auditory cues which pedestrians and cyclists use to assess the presence, proximity and location oncoming traffic may be reduced, posing a safety hazard. This laboratory s

  9. Results of measurements at a laboratory condensation heat exchanger with a corrugated minichanel tube

    Directory of Open Access Journals (Sweden)

    Jan Hrubý

    2012-04-01

    Full Text Available This article present a short selection of results obtained from measurements done at a laboratory condensation heat-exchanger with a corrugated mini-channel tube. It also touches a little the metering method and design of the heat-exchanger.

  10. Auditory localisation of conventional and electric cars : laboratory results and implications for cycling safety.

    NARCIS (Netherlands)

    Stelling-Konczak, A. Hagenzieker, M.P. Commandeur, J.J.F. Agterberg, M.J.H. & Wee, B. van

    2016-01-01

    When driven at low speeds, cars operating in electric mode have been found to be quieter than conventional cars. As a result, the auditory cues which pedestrians and cyclists use to assess the presence, proximity and location oncoming traffic may be reduced, posing a safety hazard. This laboratory

  11. Admission Laboratory Results to Enhance Prediction Models of Postdischarge Outcomes in Cardiac Care.

    Science.gov (United States)

    Pine, Michael; Fry, Donald E; Hannan, Edward L; Naessens, James M; Whitman, Kay; Reband, Agnes; Qian, Feng; Schindler, Joseph; Sonneborn, Mark; Roland, Jaclyn; Hyde, Linda; Dennison, Barbara A

    Predictive modeling for postdischarge outcomes of inpatient care has been suboptimal. This study evaluated whether admission numerical laboratory data added to administrative models from New York and Minnesota hospitals would enhance the prediction accuracy for 90-day postdischarge deaths without readmission (PD-90) and 90-day readmissions (RA-90) following inpatient care for cardiac patients. Risk-adjustment models for the prediction of PD-90 and RA-90 were designed for acute myocardial infarction, percutaneous cardiac intervention, coronary artery bypass grafting, and congestive heart failure. Models were derived from hospital claims data and were then enhanced with admission laboratory predictive results. Case-level discrimination, goodness of fit, and calibration were used to compare administrative models (ADM) and laboratory predictive models (LAB). LAB models for the prediction of PD-90 were modestly enhanced over ADM, but negligible benefit was seen for RA-90. A consistent predictor of PD-90 and RA-90 was prolonged length of stay outliers from the index hospitalization.

  12. Quality-assurance results for routine water analysis in US Geological Survey laboratories, water year 1991

    Science.gov (United States)

    Maloney, T.J.; Ludtke, A.S.; Krizman, T.L.

    1994-01-01

    The US. Geological Survey operates a quality- assurance program based on the analyses of reference samples for the National Water Quality Laboratory in Arvada, Colorado, and the Quality of Water Service Unit in Ocala, Florida. Reference samples containing selected inorganic, nutrient, and low ionic-strength constituents are prepared and disguised as routine samples. The program goal is to determine precision and bias for as many analytical methods offered by the participating laboratories as possible. The samples typically are submitted at a rate of approximately 5 percent of the annual environmental sample load for each constituent. The samples are distributed to the laboratories throughout the year. Analytical data for these reference samples reflect the quality of environmental sample data produced by the laboratories because the samples are processed in the same manner for all steps from sample login through data release. The results are stored permanently in the National Water Data Storage and Retrieval System. During water year 1991, 86 analytical procedures were evaluated at the National Water Quality Laboratory and 37 analytical procedures were evaluated at the Quality of Water Service Unit. An overall evaluation of the inorganic (major ion and trace metal) constituent data for water year 1991 indicated analytical imprecision in the National Water Quality Laboratory for 5 of 67 analytical procedures: aluminum (whole-water recoverable, atomic emission spectrometric, direct-current plasma); calcium (atomic emission spectrometric, direct); fluoride (ion-exchange chromatographic); iron (whole-water recoverable, atomic absorption spectrometric, direct); and sulfate (ion-exchange chromatographic). The results for 11 of 67 analytical procedures had positive or negative bias during water year 1991. Analytical imprecision was indicated in the determination of two of the five National Water Quality Laboratory nutrient constituents: orthophosphate as phosphorus and

  13. Randomization in laboratory procedure is key to obtaining reproducible microarray results.

    Directory of Open Access Journals (Sweden)

    Hyuna Yang

    Full Text Available The quality of gene expression microarray data has improved dramatically since the first arrays were introduced in the late 1990s. However, the reproducibility of data generated at multiple laboratory sites remains a matter of concern, especially for scientists who are attempting to combine and analyze data from public repositories. We have carried out a study in which a common set of RNA samples was assayed five times in four different laboratories using Affymetrix GeneChip arrays. We observed dramatic differences in the results across laboratories and identified batch effects in array processing as one of the primary causes for these differences. When batch processing of samples is confounded with experimental factors of interest it is not possible to separate their effects, and lists of differentially expressed genes may include many artifacts. This study demonstrates the substantial impact of sample processing on microarray analysis results and underscores the need for randomization in the laboratory as a means to avoid confounding of biological factors with procedural effects.

  14. Prescription Patterns and Mineral Metabolism Abnormalities in the Cinacalcet Era: Results from the MBD-5D Study

    Science.gov (United States)

    Fukagawa, Masafumi; Fukuma, Shingo; Onishi, Yoshihiro; Yamaguchi, Takuhiro; Hasegawa, Takeshi; Akizawa, Tadao; Kurokawa, Kiyoshi

    2012-01-01

    Summary Background and objectives Prescription patterns for hemodialysis patients with secondary hyperparathyroidism have varied widely since market introduction of cinacalcet. This study examined associations between prescription patterns and subsequent laboratory values. Design, setting, participants, & measurements Using a Mineral and Bone Disorder Outcomes Study for Japanese CKD Stage 5D Patients subcohort, 1716 prevalent hemodialysis patients (4048 sets for repeated measures between January 2008 and July 2009) with an intact parathyroid hormone (iPTH) level >180 pg/ml who used intravenous vitamin D receptor activator (VDRA) without cinacalcet were selected. Prescription patterns were defined based on cinacalcet administration (starting or not) and VDRA dosage change (decreased [25%]). Proportion differences (PDs) were determined for decreasing iPTH levels by at least one category (<180, 180–299, 300–499, and ≥500 pg/ml) and for achieving target phosphorus (3.5–6.0 mg/dl) and calcium (8.4–10.0 mg/dl) levels, adjusting for potential confounders. Results The starting cinacalcet and increased VDRA patterns were associated with decreasing iPTH levels (PD, 0.25 and 0.13; 95% confidence intervals [95% CIs], 0.19–0.31 and 0.09–0.17, respectively); combination use had an additive association (PD, 0.34; 95% CI, 0.20–0.42). The starting cinacalcet and decreased VDRA combination was associated with simultaneously achieving target phosphorus (PD, 0.12; 95% CI: 0.04–0.20) and calcium (PD, 0.09; 95% CI, 0.01–0.17) levels. Conclusions Certain combinations of cinacalcet and VDRA were associated with decreasing iPTH and achieving targets for phosphorus and calcium. Combinations may prove advantageous versus VDRA alone in managing secondary hyperparathyroidism. PMID:22822017

  15. Test Results From The Idaho National Laboratory Of The NASA Bi-Supported Cell Design

    Energy Technology Data Exchange (ETDEWEB)

    C Stoots; J O' Brien; T Cable

    2009-11-01

    The Idaho National Laboratory has been researching the application of solid-oxide fuel cell technology for large-scale hydrogen production. As a result, the Idaho National Laboratory has been testing various cell designs to characterize electrolytic performance. NASA, in conjunction with the University of Toledo, has developed a new cell concept with the goals of reduced weight and high power density. This paper presents results of the INL's testing of this new solid oxide cell design as an electrolyzer. Gas composition, operating voltage, and other parameters were varied during testing. Results to date show the NASA cell to be a promising design for both high power-to-weight fuel cell and electrolyzer applications.

  16. Risk assessment models in genetics clinic for array comparative genomic hybridization: Clinical information can be used to predict the likelihood of an abnormal result in patients.

    Science.gov (United States)

    Marano, Rachel M; Mercurio, Laura; Kanter, Rebecca; Doyle, Richard; Abuelo, Dianne; Morrow, Eric M; Shur, Natasha

    2013-03-01

    Array comparative genomic hybridization (aCGH) testing can diagnose chromosomal microdeletions and duplications too small to be detected by conventional cytogenetic techniques. We need to consider which patients are more likely to receive a diagnosis from aCGH testing versus patients that have lower likelihood and may benefit from broader genome wide scanning. We retrospectively reviewed charts of a population of 200 patients, 117 boys and 83 girls, who underwent aCGH testing in Genetics Clinic at Rhode Island hospital between 1 January/2008 and 31 December 2010. Data collected included sex, age at initial clinical presentation, aCGH result, history of seizures, autism, dysmorphic features, global developmental delay/intellectual disability, hypotonia and failure to thrive. aCGH analysis revealed abnormal results in 34 (17%) and variants of unknown significance in 24 (12%). Patients with three or more clinical diagnoses had a 25.0% incidence of abnormal aCGH findings, while patients with two or fewer clinical diagnoses had a 12.5% incidence of abnormal aCGH findings. Currently, we provide families with a range of 10-30% of a diagnosis with aCGH testing. With increased clinical complexity, patients have an increased probability of having an abnormal aCGH result. With this, we can provide individualized risk estimates for each patient.

  17. Psychosocial Determinants of Mammography Follow-up after Receipt of Abnormal Mammography Results in Medically Underserved Women

    Science.gov (United States)

    Fair, Alecia Malin; Wujcik, Debra; Lin, Jin-Mann Sally; Zheng, Wei; Egan, Kathleen M.; Grau, Ana M.; Champion, Victoria L.; Wallston, Kenneth A.

    2010-01-01

    This article targets the relationship between psychosocial determinants and abnormal screening mammography follow-up in a medically underserved population. Health belief scales were modified to refer to diagnostic follow-up versus annual screening. A retrospective cohort study design was used. Statistical analyses were performed examining relationships among sociodemographic factors, psychosocial determinants, and abnormal mammography follow-up. Women with lower mean internal health locus of control scores (3.14) were two times more likely than women with higher mean internal health locus of control scores (3.98) to have inadequate follow-up (OR = 2.53, 95% CI = 1.12–5.36). Women with less than a high school education had lower cancer fatalism scores than women who had completed high school (47.5 vs. 55.2, p-value = .02) and lower mean external health locus of control scores (3.0 vs. 5.3) (p-valueconcepts is warranted. PMID:20173286

  18. [Resistance of gonococci in the Netherlands; results of a survey of medical microbiology laboratories].

    Science.gov (United States)

    van Loo, I H M; Spaargaren, J; van de Laar, M J W

    2005-05-28

    To collect information about the incidence ofgonorrhoea and gonococcal resistance in the Netherlands. A questionnaire was sent to 39 medical microbiology laboratories to obtain information on current diagnostics and the susceptibility testing method, and on the number of positive results and the susceptibility pattern of gonococcal isolates in 2002 and 2003 (up to and including November). 32 laboratories participated in this survey. 13 laboratories used culture alone and 19 laboratories used culture and/or a molecular test. Gonorrhoea was diagnosed 2,666 times in 2002 and 2,190 times in 2003, with an incidence of 33.5 and 27.0 per 100,000 inhabitants, respectively. The rate of resistance to beta-lactam antibiotics (penicillin and amoxicillin) was 12.2% and 10.7% in 2002 and 2003, respectively, and the rates of resistance to tetracycline were 18.5% and 20.6%. An increase in the resistance to quinolones was observed from 6.6% in 2002 to 9.5% in 2003. Resistance to cephalosporins was low (0.5% in 2002 and 1.2% in 2003). Furthermore, regional differences in susceptibility were found within the Netherlands. The observed gonococcal incidence and resistance form the basis for a gonorrhoea prevention and treatment programme in the Netherlands.

  19. Coal-water-slurry evaluation. Volume 2. Laboratory and combustion test results. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Daley, R.D.; Farthing, G.A.; Vecci, S.J.

    1984-02-01

    This is the second of three volumes describing a multi-phase coal-water slurry (CWS) test program. This volume presents the results of laboratory, atomization, and combustion tests which were performed on six slurries and their parent coals. The objectives of these tests was to establish laboratory test procedures for evaluating CWS fuels, to investigate relationships between laboratory properties and CWS combustion and handling characteristics and to develop preliminary guidelines for CWS specifications. These tests showed that the preparation processes and chemical additives used by the slurry manufacturers had a significant effect on viscosity and atomization properties. The most important factor for good combustion performance was droplet size, but droplet size did not correlate with viscosity measured at low shear rates in the laboratory tests. It was also found that some slurries had greater fouling potential than their parent coals due to the use of sodium-containing additives. Tests were also conducted to determine whether the slurries could be transported and stored without coal settling. These tests showed that little settling occurred during either transportation or storage for at least three weeks. 98 figures, 27 tables.

  20. Immunostaining Results of Growth Hormone Secreting Adenomas and Their Correlation with Laboratory Findings

    Directory of Open Access Journals (Sweden)

    Mazhar Müslüm Tuna

    2016-09-01

    Full Text Available Purpose: Depending on improvements in immunohistochemical and ultrastructural analyses, hormonal immunoreactivity are more commonly detected in pituitary adenomas. In this study, we investigated the correlation between immunohistochemical results and clinical and laboratory findings. Material and Method: The records of 110 patients with acromegaly who were operated at our hospital were retrospectively evaluated. Of the patients, 43 were excluded because of missing data. All patients were clinically evaluated preoperatively and had pituitary hormone profile. Results: Our study population was consisted of 42 females and 25 males. While there were 30 patients with growth hormone staining alone, plurihormonal staining was determined at the remaining 37 patients. Three of 14 patients were diagnosed with thyrotropin-secreting pituitary adenoma according to clinical and findings laboratory, preoperatively. Except these cases, there were no hormone-secreting adenomas even if positive cellular immunreactivity existed. Discussion: Our data suggest that immunohistochemical results may not be correlated with the signs of hormone hypersecretion as evident by symptoms or laboratory results in patients with acromegaly.

  1. Laboratory test result interpretation for primary care doctors in South Africa

    Directory of Open Access Journals (Sweden)

    Naadira Vanker

    2017-02-01

    Full Text Available Background: Challenges and uncertainties with test result interpretation can lead to diagnostic errors. Primary care doctors are at a higher risk than specialists of making these errors, due to the range in complexity and severity of conditions that they encounter.Objectives: This study aimed to investigate the challenges that primary care doctors face with test result interpretation, and to identify potential countermeasures to address these.Methods: A survey was sent out to 7800 primary care doctors in South Africa. Questionnaire themes included doctors’ uncertainty with interpreting test results, mechanisms used to overcome this uncertainty, challenges with appropriate result interpretation, and perceived solutions for interpreting results.Results: Of the 552 responses received, the prevalence of challenges with result interpretation was estimated in an average of 17% of diagnostic encounters. The most commonly-reported challenges were not receiving test results in a timely manner (51% of respondents and previous results not being easily available (37%. When faced with diagnostic uncertainty, 84% of respondents would either follow-up and reassess the patient or discuss the case with a specialist, and 67% would contact a laboratory professional. The most useful test utilisation enablers were found to be: interpretive comments (78% of respondents, published guidelines (74%, and a dedicated laboratory phone line (72%.Conclusion: Primary care doctors acknowledge uncertainty with test result interpretation. Potential countermeasures include the addition of patient-specific interpretive comments, the availability of guidelines or algorithms, and a dedicated laboratory phone line. The benefit of enhanced test result interpretation would reduce diagnostic error rates. 

  2. Results of Laboratory and Industrial Tests of Periodic-Type Gas Generators

    Science.gov (United States)

    Karp, I. N.; P‧yanykh, K. E.; Antoshchuk, T. A.; Lysenko, A. A.

    2015-05-01

    Results of laboratory and industrial tests of periodic-type gas generators burning various solid biofuels have been presented. The tests were carried out with the aim of obtaining producer gas which could totally or partly replace natural gas in power equipment burning gaseous fuel. The energy and environmental characteristics of a boiler unit burning a mixture of producer gas and natural gas have been assessed.

  3. Results from laboratory tests of the two-dimensional Time-Encoded Imaging System.

    Energy Technology Data Exchange (ETDEWEB)

    Marleau, Peter; Brennan, James S.; Brubaker, Erik; Gerling, Mark D; Le Galloudec, Nathalie Joelle

    2014-09-01

    A series of laboratory experiments were undertaken to demonstrate the feasibility of two dimensional time-encoded imaging. A prototype two-dimensional time encoded imaging system was designed and constructed. Results from imaging measurements of single and multiple point sources as well as extended source distributions are presented. Time encoded imaging has proven to be a simple method for achieving high resolution two-dimensional imaging with potential to be used in future arms control and treaty verification applications.

  4. Labtracker+, a medical smartphone app for the interpretation of consecutive laboratory results: an external validation study.

    Science.gov (United States)

    Hilderink, Judith M; Rennenberg, Roger J M W; Vanmolkot, Floris H M; Bekers, Otto; Koopmans, Richard P; Meex, Steven J R

    2017-09-01

    When monitoring patients over time, clinicians may struggle to distinguish 'real changes' in consecutive blood parameters from so-called natural fluctuations. In practice, they have to do so by relying on their clinical experience and intuition. We developed Labtracker+, a medical app that calculates the probability that an increase or decrease over time in a specific blood parameter is real, given the time between measurements. We presented patient cases to 135 participants to examine whether there is a difference between medical students, residents and experienced clinicians when it comes to interpreting changes between consecutive laboratory results. Participants were asked to interpret if changes in consecutive laboratory values were likely to be 'real' or rather due to natural fluctuations. The answers of the study participants were compared with the calculated probabilities by the app Labtracker+ and the concordance rates were assessed. Medical students (n=92), medical residents from the department of internal medicine (n=19) and internists (n=24) at a Dutch University Medical Centre. Concordance rates between the study participants and the calculated probabilities by the app Labtracker+ were compared. Besides, we tested whether physicians with clinical experience scored better concordance rates with the app Labtracker+ than inexperienced clinicians. Medical residents and internists showed significantly better concordance rates with the calculated probabilities by the app Labtracker+ than medical students, regarding their interpretation of differences between consecutive laboratory results (p=0.009 and p<0.001, respectively). The app Labtracker+ could serve as a clinical decision tool in the interpretation of consecutive laboratory test results and could contribute to rapid recognition of parameter changes by physicians. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial

  5. Discordant results from reverse sequence syphilis screening--five laboratories, United States, 2006-2010.

    Science.gov (United States)

    2011-02-11

    CDC recommends syphilis serologic screening with a nontreponemal test, such as the rapid plasma reagin (RPR) or Venereal Disease Research Laboratory (VDRL) test, to identify persons with possible untreated infection; this screening is followed by confirmation using one of several treponemal tests. Recently, the availability of automatable treponemal enzyme and chemiluminescence immunoassays (EIA/CIA) has led some laboratories to adopt a reverse sequence of screening in which a treponemal EIA/CIA is performed first, followed by testing of reactive sera with a nontreponemal test. To better understand the performance of reverse sequence screening for syphilis, CDC analyzed data from five laboratories that used reverse sequence screening during 2006-2010. This report describes the results of that analysis, which indicated that among sera reactive on initial screening with a treponemal EIA/CIA, 56.7% had a nonreactive RPR test. Among these discordant sera, 31.6% also were nonreactive by treponemal testing using Treponema pallidum particle agglutination (TP-PA) or fluorescent treponemal antibody absorbed (FTA-ABS) tests. Among discordant sera, the rate of nonreactive confirmatory treponemal tests was 2.9 times higher in a population with low prevalence of syphilis, suggesting that the low-prevalence population had a higher percentage of false-positive test results. Although CDC continues to recommend the traditional algorithm with reactive nontreponemal tests confirmed by treponemal testing, in this report CDC offers additional recommendations if reverse sequence syphilis screening is used.

  6. Clinical results of stereotactic hellium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Levy, R.P.; Fabrikant, J.I.; Lyman, J.T.; Frankel, K.A.; Phillips, M.H.; Lawrence, J.H.; Tobias, C.A.

    1989-12-01

    The first therapeutic clinical trial using accelerated heavy-charged particles in humans was performed at Lawrence Berkeley Laboratory (LBL) for the treatment of various endocrine and metabolic disorders of the pituitary gland, and as suppressive therapy for adenohypophyseal hormone-responsive carcinomas and diabetic retinopathy. In acromegaly, Cushing's disease, Nelson's syndrome and prolactin-secreting tumors, the therapeutic goal in the 433 patients treated has been to destroy or inhibit the growth of the pituitary tumor and control hormonal hypersecretion, while preserving a functional rim of tissue with normal hormone-secreting capacity, and minimizing neurologic injury. An additional group of 34 patients was treated for nonsecreting chromophobe adenomas. This paper discusses the methods and results of stereotactic helium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory. 11 refs.

  7. Intercomparison of in vivo monitoring systems in Europe. Results from Risoe National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Lauridsen, B.; Soegaard-Hansen, J.

    1996-12-01

    This report contains the contribution from Risoe National Laboratory to the European project: `Intercomparison of in Vivo Monitoring Systems in Europe`. The whole-body counter at Risoe and the measurement on a phantom used as an intercalibration object in the project is described. In four case studies, prepared by the project coordinator, intakes of radionuclides and resulting doses are calculated. These calculations are based on informations on the radioactive materials taken into the body, routes of intake and on body contents of radionuclides from simulated single or multiple whole-body measurement. The answer from Risoe National Laboratory to two questionnaires - one on the whole-body counting facility and calibration methods and one on the legal requirements is the country - is listed. (au).

  8. Are laboratory derived toxicity results informative for field situations? Case study on earthworm populations contaminated with heavy metals

    NARCIS (Netherlands)

    Klok, T.C.; Thissen, J.

    2009-01-01

    The relevance of laboratory tests on toxicants for field situations is often disputed given that laboratory tests are conducted under, next to the toxicant stress, optimal conditions which are not expected in field situations. In this paper we confront the results of laboratory tests on growth,

  9. Linking accelerated laboratory and outdoor exposure results for PV polymeric materials: a mechanistic study of EVA

    Science.gov (United States)

    Gu, Xiaohong; Pang, Yongyan; Lin, Chiao-Chi; Liu, Kaipeng; Nguyen, Tinh; Chin, Jaonnie W.

    2013-09-01

    Linking accelerated laboratory test to field performance for predicting the service life of polymeric materials are being investigated at NIST using the reliability-based methodology. Based on this methodology, a successful linkage between the laboratory and field exposure data for a model polymeric material has been made. Recently, this methodology, for the first time, was introduced to the lifetime assessment of PV polymeric materials. In this paper, a mechanistic study of the degradation of three unstabilized model ethylene vinyl acetate (EVA) systems---uncured EVA, cured EVA and laminated EVA---was carried out under accelerated laboratory exposure and outdoor exposure. The NIST SPHERE (Simulated Photodegradation via High Energy Radiant Exposure) was used for the accelerated laboratory tests, and the outdoor exposure was conducted in Gaithersburg, Maryland. Simultaneous multiple stresses, including temperature, relative humidity and UV radiation, were applied individually or in combination during SPHERE exposure. The effects of the environmental factors on the main degradation mechanisms of different EVA systems were investigated. The results showed that the UV radiation was the most important factor for the degradation of EVA and a synergistic effect occurred between UV radiation and relative humidity. A slower degradation rate was observed for the laminated system as a result of limited diffusion of O2 and H2O into EVA. It was also found that the substantial chemical changes of the uncured EVA system did not yield yellowing, which was dramatically different from the peroxide cured EVA system. Additionally, the chemical degradation modes of the three EVA systems exposed outdoors appeared to be similar to those exposed to the SPHERE. The implication of this work to the current test standards was discussed.

  10. Meiotic abnormalities

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-12-31

    Chapter 19, describes meiotic abnormalities. These include nondisjunction of autosomes and sex chromosomes, genetic and environmental causes of nondisjunction, misdivision of the centromere, chromosomally abnormal human sperm, male infertility, parental age, and origin of diploid gametes. 57 refs., 2 figs., 1 tab.

  11. High acceptability for cell phone text messages to improve communication of laboratory results with HIV-infected patients in rural Uganda: a cross-sectional survey study

    Directory of Open Access Journals (Sweden)

    Siedner Mark J

    2012-06-01

    Full Text Available Abstract Background Patient-provider communication is a major challenge in resource-limited settings with large catchment areas. Though mobile phone usership increased 20-fold in Africa over the past decade, little is known about acceptability of, perceptions about disclosure and confidentiality, and preferences for cell phone communication of health information in the region. Methods We performed structured interviews of fifty patients at the Immune Suppression Syndrome clinic in Mbarara, Uganda to assess four domains of health-related communication: a cell phone use practices and literacy, b preferences for laboratory results communication, c privacy and confidentiality, and d acceptability of and preferences for text messaging to notify patients of abnormal test results. Results Participants had a median of 38 years, were 56% female, and were residents of a large catchment area throughout southwestern Uganda. All participants expressed interest in a service to receive information about laboratory results by cell phone text message, stating benefits of increased awareness of their health and decreased transportation costs. Ninety percent reported that they would not be concerned for unintended disclosure. A minority additionally expressed concerns about difficulty interpreting messages, discouragement upon learning bad news, and technical issues. Though all respondents expressed interest in password protection of messages, there was also a strong desire for direct messages to limit misinterpretation of information. Conclusions Cell phone text messaging for communication of abnormal laboratory results is highly acceptable in this cohort of HIV-infected patients in rural Uganda. The feasibility of text messaging, including an optimal balance between privacy and comprehension, should be further studied.

  12. Integrating monitor alarms with laboratory test results to enhance patient deterioration prediction.

    Science.gov (United States)

    Bai, Yong; Do, Duc H; Harris, Patricia Rae Eileen; Schindler, Daniel; Boyle, Noel G; Drew, Barbara J; Hu, Xiao

    2015-02-01

    Patient monitors in modern hospitals have become ubiquitous but they generate an excessive number of false alarms causing alarm fatigue. Our previous work showed that combinations of frequently co-occurring monitor alarms, called SuperAlarm patterns, were capable of predicting in-hospital code blue events at a lower alarm frequency. In the present study, we extend the conceptual domain of a SuperAlarm to incorporate laboratory test results along with monitor alarms so as to build an integrated data set to mine SuperAlarm patterns. We propose two approaches to integrate monitor alarms with laboratory test results and use a maximal frequent itemsets mining algorithm to find SuperAlarm patterns. Under an acceptable false positive rate FPRmax, optimal parameters including the minimum support threshold and the length of time window for the algorithm to find the combinations of monitor alarms and laboratory test results are determined based on a 10-fold cross-validation set. SuperAlarm candidates are generated under these optimal parameters. The final SuperAlarm patterns are obtained by further removing the candidates with false positive rate>FPRmax. The performance of SuperAlarm patterns are assessed using an independent test data set. First, we calculate the sensitivity with respect to prediction window and the sensitivity with respect to lead time. Second, we calculate the false SuperAlarm ratio (ratio of the hourly number of SuperAlarm triggers for control patients to that of the monitor alarms, or that of regular monitor alarms plus laboratory test results if the SuperAlarm patterns contain laboratory test results) and the work-up to detection ratio, WDR (ratio of the number of patients triggering any SuperAlarm patterns to that of code blue patients triggering any SuperAlarm patterns). The experiment results demonstrate that when varying FPRmax between 0.02 and 0.15, the SuperAlarm patterns composed of monitor alarms along with the last two laboratory test results

  13. Thyroid function testing in primary care: overused and under-evidenced? A study examining which clinical features correspond to an abnormal thyroid function result.

    Science.gov (United States)

    Werhun, Alexander; Hamilton, William

    2015-04-01

    Diagnostic testing is increasing in primary care, including for thyroid disease. This study examined which clinical features were associated with an abnormal thyroid stimulating hormone (TSH) result. This was a cross-sectional study in one general practice of 16,487 patients in Exeter, Devon, UK. We examined the primary care records relating to every TSH test taken in the year from August 2012, and extracted symptoms and/or the indication for testing. Associations with an abnormal result were tested using multivariable logistic regression. A cohort study was then performed of 100 patients newly recorded with each of the six features associated with an abnormal test result in the cross-sectional study, and the proportions tested for TSH and the results of that testing identified. Two thousand thirty-five patients (12% of the practice population) had TSH testing in the year. Of these 35 (1.7%) had a TSH >4.5 mIU/l, suggesting hypothyroidism, and 7 (0.3%) had TSH <0.01 mIu/l suggesting hyperthyroidism. Features associated with an abnormal TSH were: pregnancy, odds ratio 41 (95% confidence interval 9.3-180), constipation 9.7 (2.1-45), palpitations 23 (3.4-150), hair loss, 21 (2.0-230), weight gain, 18 (1.6-190) and diarrhoea, 13 (1.2-130); in separate analyses only pregnancy and constipation were associated with a raised TSH, and the remaining four features with a low TSH. The diagnostic yield of thyroid disease in this study was 2.1% suggests testing could be better targeted without missing diagnoses. The symptoms associated with thyroid disease differ from those generally reported. This may represent fewer patients presenting with advanced disease. © The Author 2015. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  14. Electrical triggering of earthquakes: results of laboratory experiments at spring-block models

    Science.gov (United States)

    Novikov, Victor A.; Okunev, Vladimir I.; Klyuchkin, Vadim N.; Liu, Jing; Ruzhin, Yuri Ya.; Shen, Xuhui

    2017-05-01

    Recently published results of field and laboratory experiments on the seismic/acoustic response to injection of direct current (DC) pulses into the Earth crust or stressed rock samples raised a question on a possibility of electrical earthquake triggering. A physical mechanism of the considered phenomenon is not clear yet in view of the very low current density (10-7-10-8 A/m2) generated by the pulsed power systems at the epicenter depth (5-10 km) of local earthquakes occurred just after the current injection. The paper describes results of laboratory "earthquake" triggering by DC pulses under conditions of a spring-block model simulated the seismogenic fault. It is experimentally shown that the electric triggering of the laboratory "earthquake" (sharp slip of a movable block of the spring-block system) is possible only within a range of subcritical state of the system, when the shear stress between the movable and fixed blocks obtains 0.98-0.99 of its critical value. The threshold of electric triggering action is about 20 A/m2 that is 7-8 orders of magnitude higher than estimated electric current density for Bishkek test site (Northern Tien Shan, Kirghizia) where the seismic response to the man-made electric action was observed. In this connection, the electric triggering phenomena may be explained by contraction of electric current in the narrow conductive areas of the faults and the corresponding increase in current density or by involving the secondary triggering mechanisms like electromagnetic stimulation of conductive fluid migration into the fault area resulted in decrease in the fault strength properties.

  15. Hepatitis E virus infection in Brazil: results of laboratory-based surveillance from 1998 to 2013

    Directory of Open Access Journals (Sweden)

    Ana Maria Passos-Castilho

    2015-01-01

    Full Text Available INTRODUCTION: Data on hepatitis E virus (HEV in Brazil are limited. We analyzed 15 years of HEV surveillance data in a major clinical laboratory in São Paulo, Brazil. METHODS: The seroprevalence of HEV of 2,271 patients subjected to anti-HEV tests from 1998 to 2013 were analyzed. RESULTS: HEV seroprevalence was 2.1%, and the anti-HEV IgM positivity rate was 4.9%. Six hepatitis E patients were identified. CONCLUSIONS: HEV seroprevalence and detection rates appear to have increased in recent years. Hepatitis E should be investigated further and included in the differential diagnosis of hepatitis in Brazil.

  16. Hepatitis E virus infection in Brazil: results of laboratory-based surveillance from 1998 to 2013

    OpenAIRE

    Ana Maria Passos-Castilho; Anne de Sena; Mônica Renata Reinaldo; Celso Francisco Hernandes Granato

    2015-01-01

    INTRODUCTION: Data on hepatitis E virus (HEV) in Brazil are limited. We analyzed 15 years of HEV surveillance data in a major clinical laboratory in São Paulo, Brazil. METHODS: The seroprevalence of HEV of 2,271 patients subjected to anti-HEV tests from 1998 to 2013 were analyzed. RESULTS: HEV seroprevalence was 2.1%, and the anti-HEV IgM positivity rate was 4.9%. Six hepatitis E patients were identified. CONCLUSIONS: HEV seroprevalence and detection rates appear to have increased in recent y...

  17. First laboratory results with the LINC-NIRVANA high layer wavefront sensor

    Science.gov (United States)

    Zhang, Xianyu; Gaessler, Wolfgang; Conrad, Albert R.; Bertram, Thomas; Arcidiacono, Carmelo; Herbst, Thomas M.; Kuerster, Martin; Bizenberger, Peter; Meschke, Daniel; Rix, Hans-Walter; Rao, Changhui; Mohr, Lars; Briegel, Florian; Kittmann, Frank; Berwein, Juergen; Trowitzsch, Jan; Schreiber, Laura; Ragazzoni, Roberto; Diolaiti, Emiliano

    2011-08-01

    In the field of adaptive optics, multi-conjugate adaptive optics (MCAO) can greatly increase the size of the corrected field of view (FoV) and also extend sky coverage. By applying layer oriented MCAO (LO-MCAO) [4], together with multiple guide stars (up to 20) and pyramid wavefront sensors [7], LINC-NIRVANA (L-N for short) [1] will provide two AO-corrected beams to a Fizeau interferometer to achieve 10 milliarcsecond angular resolution on the Large Binocular Telescope. This paper presents first laboratory results of the AO performance achieved with the high layer wavefront sensor (HWS). This sensor, together with its associated deformable mirror (a Xinetics-349), is being operated in one of the L-N laboratories. AO reference stars, spread across a 2 arc-minute FoV and with aberrations resulting from turbulence introduced at specific layers in the atmosphere, are simulated in this lab environment. This is achieved with the Multi-Atmosphere Phase screen and Stars (MAPS) [2] unit. From the wavefront data, the approximate residual wavefront error after correction has been calculated for different turbulent layer altitudes and wind speeds. Using a somewhat undersampled CCD, the FWHM of stars in the nearly 2 arc-minute FoV has also been measured. These test results demonstrate that the high layer wavefront sensor of LINC-NIRVANA will be able to achieve uniform AO correction across a large FoV.

  18. Evaluation of a laboratory system intended for use in physicians' offices. I. Reliability of results produced by trained laboratory technologists.

    Science.gov (United States)

    Belsey, R; Goitein, R K; Baer, D M

    1987-07-17

    The accuracy and precision of the Kodak DT-60 tabletop chemistry analyzer were evaluated in the clinical laboratory at the Portland (Ore) Veterans Administration Medical Center, and its operational throughout and cost were estimated. All DT-60 tests that were studied exhibited clinically acceptable precision and, except for the glucose method, accuracy. The accuracy of the glucose method was indeterminate with the available data. Throughput under field conditions was found to be less than half of the manufacturer's claim. The estimated supply cost per test could vary from $1.20 to $5.49 per test, depending on the test type and the number of assays expected to be performed daily. The instrument seems to be accurate, precise, and generally reliable when operated by professional medical technologists.

  19. Detection of pathogenic protozoa in the diagnostic laboratory: result reproducibility, specimen pooling, and competency assessment.

    Science.gov (United States)

    Libman, M D; Gyorkos, T W; Kokoskin, E; Maclean, J D

    2008-07-01

    Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and which is based on the blinded resubmission of selected clinical samples, using concordance between the reports of the initial and resubmitted specimen as an indicator. Specimens preserved in sodium acetate-acetic acid-formalin can be stored for several months for use in such a program. The presence of multiple protozoa in one specimen does not affect concordance. Some dilution of specimens occurs in this process, and this may explain poor concordance when specimens with low protozoal concentrations are resubmitted. Evaluation of this tool in a large parasitology laboratory revealed concordance rates for pathogenic protozoa (Entamoeba histolytica/Entamoeba dispar, Giardia lamblia, and Dientamoeba fragilis) of about 80%, which may be considered for use as a benchmark value. We also used this tool to demonstrate that when pairs of specimens from one patient are pooled to create a single specimen, concordance between the results of the individual and pooled specimens is high.

  20. Detection of Pathogenic Protozoa in the Diagnostic Laboratory: Result Reproducibility, Specimen Pooling, and Competency Assessment▿

    Science.gov (United States)

    Libman, M. D.; Gyorkos, T. W.; Kokoskin, E.; MacLean, J. D.

    2008-01-01

    Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and which is based on the blinded resubmission of selected clinical samples, using concordance between the reports of the initial and resubmitted specimen as an indicator. Specimens preserved in sodium acetate-acetic acid-formalin can be stored for several months for use in such a program. The presence of multiple protozoa in one specimen does not affect concordance. Some dilution of specimens occurs in this process, and this may explain poor concordance when specimens with low protozoal concentrations are resubmitted. Evaluation of this tool in a large parasitology laboratory revealed concordance rates for pathogenic protozoa (Entamoeba histolytica/Entamoeba dispar, Giardia lamblia, and Dientamoeba fragilis) of about 80%, which may be considered for use as a benchmark value. We also used this tool to demonstrate that when pairs of specimens from one patient are pooled to create a single specimen, concordance between the results of the individual and pooled specimens is high. PMID:18448690

  1. Testbeam and laboratory test results of irradiated 3D CMS pixel detectors

    Energy Technology Data Exchange (ETDEWEB)

    Bubna, Mayur [Purdue University, Department of Physics, West Lafayette, IN 47907-1396 (United States); Purdue University, School of Electrical and Computer Engineering, West Lafayette, IN 47907-1396 (United States); Alagoz, Enver, E-mail: enver.alagoz@cern.ch [Purdue University, Department of Physics, West Lafayette, IN 47907-1396 (United States); Cervantes, Mayra; Krzywda, Alex; Arndt, Kirk [Purdue University, Department of Physics, West Lafayette, IN 47907-1396 (United States); Obertino, Margherita; Solano, Ada [Istituto Nazionale di Fisica Nucleare, Sezione di Torino, 10125 Torino (Italy); Dalla Betta, Gian-Franco [INFN Padova (Gruppo Collegato di Trento) (Italy); Dipartimento di Ingegneria e Scienzadella Informazione, Universitá di Trento, I-38123 Povo di Trento (Italy); Menace, Dario; Moroni, Luigi [Istituto Nazionale di Fisica Nucleare, Sezione di Milano Bicocca (Italy); Universitá degli Studi di Milano Bicocca, 20126 Milano (Italy); Uplegger, Lorenzo; Rivera, Ryan [Fermi National Accelerator Laboratory, Batavia, IL 60510-0500 (United States); Osipenkov, Ilya [Texas A and M University, Department of Physics, College Station, TX 77843-4242 (United States); Andresen, Jeff [Fermi National Accelerator Laboratory, Batavia, IL 60510-0500 (United States); Bolla, Gino; Bortoletto, Daniela [Purdue University, Department of Physics, West Lafayette, IN 47907-1396 (United States); Boscardin, Maurizio [Centro per i Materiali e i Microsistemi Fondazione Bruno Kessler (FBK), Trento, I-38123 Povo di Trento (Italy); Marie Brom, Jean [Strasbourg IPHC, Institut Pluriedisciplinaire Hubert Curien, F-67037 Strasbourg Cedex (France); Brosius, Richard [State University of New York at Buffalo (SUNY), Department of Physics, Buffalo, NY 14260-1500 (United States); Chramowicz, John [Fermi National Accelerator Laboratory, Batavia, IL 60510-0500 (United States); and others

    2013-12-21

    The CMS silicon pixel detector is the tracking device closest to the LHC p–p collisions, which precisely reconstructs the charged particle trajectories. The planar technology used in the current innermost layer of the pixel detector will reach the design limit for radiation hardness at the end of Phase I upgrade and will need to be replaced before the Phase II upgrade in 2020. Due to its unprecedented performance in harsh radiation environments, 3D silicon technology is under consideration as a possible replacement of planar technology for the High Luminosity-LHC or HL-LHC. 3D silicon detectors are fabricated by the Deep Reactive-Ion-Etching (DRIE) technique which allows p- and n-type electrodes to be processed through the silicon substrate as opposed to being implanted through the silicon surface. The 3D CMS pixel devices presented in this paper were processed at FBK. They were bump bonded to the current CMS pixel readout chip, tested in the laboratory, and testbeams carried out at FNAL with the proton beam of 120 GeV/c. In this paper we present the laboratory and beam test results for the irradiated 3D CMS pixel devices. -- Highlights: •Pre-irradiation and post-irradiation electrical properties of 3D sensors and 3D diodes from various FBK production batches were measured and analyzed. •I–T measurements of gamma irradiated diodes were analyzed to understand leakage current generation mechanism in 3D diodes. •Laboratory measurements: signal to noise ratio and charge collection efficiency of 3D sensors before and after irradiation. •Testbeam measurements: pre- and post-irradiation pixel cell efficiency and position resolution of 3D sensors.

  2. XPC branch-point sequence mutations disrupt U2 snRNP binding, resulting in abnormal pre-mRNA splicing in xeroderma pigmentosum patients.

    Science.gov (United States)

    Khan, Sikandar G; Yamanegi, Koji; Zheng, Zhi-Ming; Boyle, Jennifer; Imoto, Kyoko; Oh, Kyu-Seon; Baker, Carl C; Gozukara, Engin; Metin, Ahmet; Kraemer, Kenneth H

    2010-02-01

    Mutations in two branch-point sequences (BPS) in intron 3 of the XPC DNA repair gene affect pre-mRNA splicing in association with xeroderma pigmentosum (XP) with many skin cancers (XP101TMA) or no skin cancer (XP72TMA), respectively. To investigate the mechanism of these abnormalities we now report that transfection of minigenes with these mutations revealed abnormal XPC pre-mRNA splicing that mimicked pre-mRNA splicing in the patients' cells. DNA oligonucleotide-directed RNase H digestion demonstrated that mutations in these BPS disrupt U2 snRNP-BPS interaction. XP101TMA cells had no detectable XPC protein but XP72TMA had 29% of normal levels. A small amount of XPC protein was detected at sites of localized ultraviolet (UV)-damaged DNA in XP72TMA cells which then recruited other nucleotide excision repair (NER) proteins. In contrast, XP101TMA cells had no detectable recruitment of XPC or other NER proteins. Post-UV survival and photoproduct assays revealed greater reduction in DNA repair in XP101TMA cells than in XP72TMA. Thus mutations in XPC BPS resulted in disruption of U2 snRNP-BPS interaction leading to abnormal pre-mRNA splicing and reduced XPC protein. At the cellular level these changes were associated with features of reduced DNA repair including diminished NER protein recruitment, reduced post-UV survival and impaired photoproduct removal.

  3. First results of the IGEX dark matter experiment at the Canfranc Underground Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Cebrian, S.; Morales, A.; Aalseth, C.E.; Avignone, F.T.; Brodzinski, R.L.; Garcia, E.; Gonzalez, D.; Hensley, W.K.; Irastorza, I.G.; Kirpichnikov, I.V.; Klimenko, A.A.; Miley, H.S.; Morales, J.; Ortiz de Solorzano, A.; Osetrov, S.B.; Pogosov, V.S.; Puimedon, J.; Reeves, J.H.; Sarsa, M.L.; Scopel, S.; Smolnikov, A.A.; Tamanyan, A.G.; Vasenko, A.A.; Vasiliev, S.I.; Villar, J.A

    2001-04-01

    The enriched {sup 76}Ge double-beta decay detectors from the International Germanium EXperiment (IGEX), operating in the Canfranc Underground Laboratory with an overbuden of 2450 m.w.e., were recently upgraded to use them also in a search for WIMPs. This paper presents a description of the experiment and the data analysis as well as a new exclusion plot, {sigma}(m), derived from the IGEX data for WIMP-nucleon spin-independent interaction. To obtain this result, 30 days of data from one 2-kg IGEX detector, with an energy threshold E{sub thr} {approx} 4 keV, have been considered. These results improve the exclusion limits derived from other conventional ionization germanium experiments in the {approx} 50 GeV DAMA region.

  4. Does a dynamic chair increase office workers' movements? - Results from a combined laboratory and field study.

    Science.gov (United States)

    Grooten, Wilhelmus J A; Äng, Björn O; Hagströmer, Maria; Conradsson, David; Nero, Håkan; Franzén, Erika

    2017-04-01

    Dynamic chairs have the potential to facilitate movements that could counteract health problems associated with sedentary office work. This study aimed to evaluate whether a dynamic chair can increase movements during desk-based office work. Fifteen healthy subjects performed desk-based office work using a dynamic office chair and compared to three other conditions in a movement laboratory. In a field study, the dynamic office chair was studied during three working days using accelerometry. Equivocal results showed that the dynamic chair increased upper body and chair movements as compared to the conventional chair, but lesser movements were found compared to standing. No differences were found between the conditions in the field study. A dynamic chair may facilitate movements in static desk-based office tasks, but the results were not consistent for all outcome measures. Validation of measuring protocols for assessing movements during desk-based office work is warranted. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. An unusual case of coccidiosis in laboratory-reared pheasants resulting from a breach in biosecurity.

    Science.gov (United States)

    Gerhold, R W; Williams, S M; Fuller, A L; McDougald, L R

    2010-09-01

    An outbreak of coccidiosis in laboratory-reared Chinese ring-necked pheasants (Phasianus colchicus) resulted in high morbidity and moderate mortality. The outbreak was associated with a breach in biosecurity caused by the cleaning of a sewer line with a mechanical device, resulting in extensive splattering of fecal material throughout the "clean room" where birds were held prior to use in coccidiosis experiments. Mortality and morbidity in the affected birds were seen exactly 5 days after the incident, after birds had been moved to another room for experimental use, corresponding closely with the known prepatent or preclinical period of Eimeria phasiani and Eimeria colchici. Gross lesions in the affected birds varied from dehydration to intestinal and ventricular hemorrhage. Microscopic examination confirmed a diagnosis of severe intestinal coccidiosis. This report underscores the ease of contamination of experimental birds leading to coccidiosis outbreaks during breaches of management and biosecurity.

  6. Some causes of inter-laboratory variation in the results of comet assay.

    Science.gov (United States)

    Sirota, Nikolai P; Zhanataev, Aliy K; Kuznetsova, Elena A; Khizhnyak, Eugenii P; Anisina, Elena A; Durnev, Andrei D

    2014-08-01

    We performed an inter-laboratory study to determine the variation of comet assay results and to identify its possible reasons. An exchange of slides between Labs in different stages of the comet assay protocol was performed. Because identical slides, durations of alkali treatment and electrophoresis, and similar electric field strengths (2.0 V/cm and 2.14 V/cm) were used, we concluded that the observed inter-laboratory difference in the results is directly associated with the electrophoresis step. In Lab 1, mouse bone marrow cells were exposed to methyl methanesulfonate at concentrations of 10, 25 and 50 μM for 3 h at 37 °C. In Lab 2, cells the same as in Lab 1 were immobilized in LMA on slides and exposed to X-rays at doses of 3-8 Gy. We found that the transportation of slides after lysis or electrophoresis step, as well as different dyes used for scoring did not produce any significant effect on the results. No substantial difference in the data was also revealed when various software packages were used for image analysis. The temperature of the alkaline solution was shown to increase during electrophoresis and, besides, the temperature heterogeneity of the solution took place in the area of the platform, with a maximum in the middle of the chamber. The temperature heterogeneity could affect the rate of conversion of alkali labile sites into single stranded breaks. Thus, it was clearly indicated that real temperature variations during the alkali treatment and electrophoresis were an essential factor in the variability of the results between our Labs.

  7. Performance of the Aachen Acoustic Laboratory and results from comparative studies in water and ice

    Science.gov (United States)

    Heinen, Dirk; Paul, Larissa; Wiebusch, Christopher

    2013-05-01

    To investigate acoustic ice properties under laboratory conditions and to test the thermoacoustic model, the Aachen Acoustic Laboratory (AAL) was founded as a part of the activities of the acoustic working group (SPATS) within the IceCube collaboration. The goal of SPATS is to evaluate the possibility of acoustic detection of ultra high-energy neutrinos in the Antarctic ice. The AAL provides a test facility setup with a proper infrastructure to study acoustics and thermoacoustics in a large volume of water and ice. The control of the freezing process, the ice quality, the temperature monitoring at different phases of the medium and the laser-based thermoacoustic sound generation are the key features of the setup. The AAL setup provides the possibility for the characterization of a wide range of acoustic transducers, sensor/transmitter calibration, study of the thermoacoustic sound generation, study of the acoustic properties of the ice, water as well as the water/ice interfaces, and research and development of new types of acoustic transducers (PVDF-based) as an alternative to standard PZTs. In this document, the different parts of the AAL setup are described, results on the commissioning from the laser based thermoacoustic sound generation are presented and the performance of an absolute sensor calibration method in water and ice are discussed.

  8. Intermediate-Scale Laboratory Experiments of Subsurface Flow and Transport Resulting from Tank Leaks

    Energy Technology Data Exchange (ETDEWEB)

    Oostrom, Martinus; Wietsma, Thomas W.

    2014-09-30

    Washington River Protection Solutions contracted with Pacific Northwest National Laboratory to conduct laboratory experiments and supporting numerical simulations to improve the understanding of water flow and contaminant transport in the subsurface between waste tanks and ancillary facilities at Waste Management Area C. The work scope included two separate sets of experiments: •Small flow cell experiments to investigate the occurrence of potential unstable fingering resulting from leaks and the limitations of the STOMP (Subsurface Transport Over Multiple Phases) simulator to predict flow patterns and solute transport behavior under these conditions. Unstable infiltration may, under certain conditions, create vertically elongated fingers potentially transporting contaminants rapidly through the unsaturated zone to groundwater. The types of leak that may create deeply penetrating fingers include slow release, long duration leaks in relatively permeable porous media. Such leaks may have occurred below waste tanks at the Hanford Site. •Large flow experiments to investigate the behavior of two types of tank leaks in a simple layered system mimicking the Waste Management Area C. The investigated leaks include a relatively large leak with a short duration from a tank and a long duration leak with a relatively small leakage rate from a cascade line.

  9. Evaluation of a laboratory system intended for use in physicians' offices. II. Reliability of results produced by health care workers without formal or professional laboratory training.

    Science.gov (United States)

    Belsey, R; Vandenbark, M; Goitein, R K; Baer, D M

    1987-07-17

    The Kodak DT-60 tabletop chemistry analyzer was evaluated with standardized protocols to determine the system's precision and accuracy when operated by four volunteers (a secretary, a licensed practical nurse, and two family medicine residents) in a simulated office laboratory. The variability of the results was found to be significantly greater than the variability of results produced by medical technologists who analyzed the same samples during the same study period with another DT-60 placed in the hospital laboratory. The source(s) of increased variance needs to be identified so the system can be modified or new control procedures can be developed to ensure the reliability of results used in patient care. Prospective purchasers, manufacturers, and patients need this kind of objective information about the reliability of results produced by systems intended for use in physicians' office laboratories.

  10. Psychological impact, support and information needs for women with an abnormal Pap smear: comparative results of a questionnaire in three European countries

    Directory of Open Access Journals (Sweden)

    Jorge Anna

    2011-05-01

    Full Text Available Abstract Background Extensive information on cervical cancer is currently available. Its effectiveness in reducing anxiety in women receiving abnormal Pap tests is not clear. We investigated current practices of communicating abnormal Pap results to evaluate women's reactions and determine the sources of information they use subsequently. Methods A self-administered questionnaire-based study was performed in 1475 women in France, Spain and Portugal who had received an abnormal Pap smear result in the 12 months prior to completing the questionnaire. Questions covered methods of communication of the result, emotional reactions, support received (from the physician and entourage, and information sources, using pre-specified check box options and rating scales. Data were analyzed by country. Results Pap test results were mostly communicated by phone to Spanish women (76%, while physician letters were common in France (59% and Portugal (36%. Frequent reactions were anxiety, panic and stress, which were less common in Spanish women than their French and Portuguese counterparts. After discussing with their physician, half of the participants were worried, despite rating highly the psychological support received. Over 90% of women in each country discussed their results with family or friends. Partners provided a high level of support. Overall, the abnormal diagnosis and consequences had a low to medium impact on daily, professional and family life and their relationships with their partner. Impact was higher in Spanish women than the French or Portuguese. Information on the diagnosis and its treatment was rated average, and nearly 80% of participants wanted more information, notably French women. Preferred sources were the physician and the Internet. Conclusions Women expressed a strong wish for more information about cervical cancer and other HPV-related diseases, and that their physician play a major role in its provision and in support. There was a

  11. Laboratory and test beam results from a large-area silicon drift detector

    CERN Document Server

    Bonvicini, V; Giubellino, P; Gregorio, A; Idzik, M; Kolojvari, A A; Montaño-Zetina, L M; Nouais, D; Petta, C; Rashevsky, A; Randazzo, N; Reito, S; Tosello, F; Vacchi, A; Vinogradov, L I; Zampa, N

    2000-01-01

    A very large-area (6.75*8 cm/sup 2/) silicon drift detector with integrated high-voltage divider has been designed, produced and fully characterised in the laboratory by means of ad hoc designed MOS injection electrodes. The detector is of the "butterfly" type, the sensitive area being subdivided into two regions with a maximum drift length of 3.3 cm. The device was also tested in a pion beam (at the CERN PS) tagged by means of a microstrip detector telescope. Bipolar VLSI front-end cells featuring a noise of 250 e/sup -/ RMS at 0 pF with a slope of 40 e/sup -//pF have been used to read out the signals. The detector showed an excellent stability and featured the expected characteristics. Some preliminary results will be presented. (12 refs).

  12. The preliminary results of fast neutron flux measurements in the DULB laboratory at Baksan

    CERN Document Server

    Abdurashitov, J N; Kalikhov, A V; Shikhin, A A; Yants, V E; Zaborskaia, O S; Klimenko, A A; Osetrov, S B; Smolnikov, A A; Vasilev, S I

    2000-01-01

    One of the main sources of a background in underground physics experiments (such as the investigation of solar neutrino flux, neutrino oscillations, neutrinoless double beta decay, and the search for annual and daily Cold Dark Matter particle flux modulation) are fast neutrons originating from the surrounding rocks. The measurements of fast neutron flux in the new DULB Laboratory situated at a depth of 4900 m w.e. in the Baksan Neutrino Observatory have been performed. The relative neutron shielding properties of several commonly available natural materials were investigated too. The preliminary results obtained with a high-sensitive fast neutron spectrometer at the level of sensitivity of about 10^(-7) neutron/ (cm^2 sec) are presented and discussed.

  13. Post-depositional changes in snow isotope content: preliminary results of laboratory experiments

    Directory of Open Access Journals (Sweden)

    A. A. Ekaykin

    2009-10-01

    Full Text Available Isotopic content of the snow and firn thickness is assumed to be altered significantly due to the post-depositional (PD mass- and isotope exchange with the atmospheric water vapor. If so, these effects should be accounted for in the ice core-based isotope-temperature paleo-reconstructions. In order to study the intensity of the PD processes we set up a series of laboratory experiments. In this paper we describe in detail the experimental technique and briefly overview preliminary results. It is shown that the PD modifications in the upper layer of snow thickness are noticeably strong even under such a low temperature as −35°C (the value typical for the Central Antarctic summer. It is demonstrated that the PD isotopic changes in snow can be approximated as a linear function of the relative mass loss due to snow sublimation. Possible applications for improving the isotope-temperature paleo-reconstructions are shortly discussed.

  14. Results from Sandia National Laboratories/Lockheed Martin Electromagnetic Missile Launcher (EMML).

    Energy Technology Data Exchange (ETDEWEB)

    Lockner, Thomas Ramsbeck; Skurdal, Ben (Lockheed Martin MS2, Baltimore, MD); Gaigler, Randy (Lockheed Martin MS2, Baltimore, MD); Basak, L (Lockheed Martin MS2, Baltimore, MD); Root, G (Lockheed Martin MS2, Baltimore, MD); Aubuchon, Matthew S.; Turman, Bobby N.; Floyd, Mendel D. (Lockheed Martin MS2, Baltimore, MD)

    2005-05-01

    Sandia national laboratories (SNL) and lockheed martin MS2 are designing an electromagnetic missile launcher (EMML) for naval applications. The EMML uses an induction coilgun topology with the requirement of launching a 3600 lb. missile up to a velocity of 40 m/s. To demonstrate the feasibility of the electromagnetic propulsion design, a demonstrator launcher was built that consists of approximately 10% of the propulsion coils needed for a tactical design. The demonstrator verified the design by launching a 1430 lb weighted sled to a height of 24 ft in mid-December 2004 (Figure 1). This paper provides the general launcher design, specific pulsed power system component details, system operation, and demonstration results.

  15. Area Monitoring Dosimeter Program for the Pacific Northwest National Laboratory: Results for CY 2005

    Energy Technology Data Exchange (ETDEWEB)

    Bivins, Steven R.; Stoetzel, Gregory A.

    2006-06-21

    Pacific Northwest National Laboratory (PNNL) established an area monitoring dosimeter program in accordance with Article 514 of the Department of Energy (DOE) Radiological Control Manual (RCM) in January 1993. This program is to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological Buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a)(1)-(4) and Article 511.1 of the PNNL Radiological Control Program Description, personnel dosimetry shall be provided to (1) radiological workers who are likely to receive at least 100 mrem annually, and (2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years 1993-2005 confirm that personnel dosimetry is not needed for individuals located in areas monitored by the program

  16. Area Monitoring Dosimeter Program for the Pacific Northwest National Laboratory: Results for CY 2001

    Energy Technology Data Exchange (ETDEWEB)

    Bivins, Steven R.; Stoetzel, Gregory A.

    2002-07-08

    Pacific Northwest National Laboratory (PNNL) established an area monitoring dosimeter program in accordance with Article 514 of the U.S. Department of Energy (DOE) Radiological Control Manual (RCM) in January 1993. This program is to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological Buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a) (1)-(4) and Article 511.1 of the PNNL Radiological Control Program Description, personnel dosimetry shall be provided to 1) radiological workers who are likely to receive at least 100 mrem annually, and 2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years 1993-2001 confirm that personnel dosimetry is not needed for individuals located in areas monitored by the program.

  17. Area Monitoring Dosimeter Program for the Pacific Northwest National Laboratory: Results for CY 2006

    Energy Technology Data Exchange (ETDEWEB)

    Bivins, Steven R.; Stoetzel, Gregory A.

    2007-07-19

    Pacific Northwest National Laboratory (PNNL) established an area monitoring dosimeter program in accordance with Article 514 of the Department of Energy (DOE) Radiological Control Manual (RCM) in January 1993. This program is to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological Buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a)(1)-(4) and Article 511.1 of the PNNL Radiological Control Program Description, personnel dosimetry shall be provided to 1) radiological workers who are likely to receive at least 100 mrem annually, and 2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years 1993-2005 confirm that personnel dosimetry is not needed for individuals located in areas monitored by the program.

  18. Area Monitoring Dosimeter Program for the Pacific Northwest National Laboratory: Results for CY 2000

    Energy Technology Data Exchange (ETDEWEB)

    Bivins, Steven R.; Stoetzel, Gregory A.

    2001-07-05

    Pacific Northwest National Laboratory (PNNL) established an area monitoring dosimeter program in accordance with Article 514 of the Department of Energy (DOE) Radiological Control Manual (RCM) in January 1993. This program is to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological Buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a) (1)-(4) and Article 511.1 of the DOE Standard Radiological Control, personnel dosimetry shall be provided to 1) radiological workers who are likely to receive at least 100 mrem annually, and 2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years 1993-2000 confirm that personnel dosimetry is not needed for individuals located in areas monitored by the program.

  19. Hepatitis E virus infection in Brazil: results of laboratory-based surveillance from 1998 to 2013

    Directory of Open Access Journals (Sweden)

    Ana Maria Passos-Castilho

    2015-08-01

    Full Text Available INTRODUCTION:Data on hepatitis E virus (HEV in Brazil are limited. We analyzed 15 years of HEV surveillance data in a major clinical laboratory in São Paulo, Brazil.METHODS:The seroprevalence of HEV of 2,271 patients subjected to anti-HEV tests from 1998 to 2013 were analyzed.RESULTS:HEV seroprevalence was 2.1%, and the anti-HEV IgM positivity rate was 4.9%. Six hepatitis E patients were identified.CONCLUSIONS:HEV seroprevalence and detection rates appear to have increased in recent years. Hepatitis E should be investigated further and included in the differential diagnosis of hepatitis in Brazil.

  20. Effectiveness of an electronic health record-based intervention to improve follow-up of abnormal pathology results: a retrospective record analysis.

    Science.gov (United States)

    Laxmisan, Archana; Sittig, Dean F; Pietz, Kenneth; Espadas, Donna; Krishnan, Bhuvaneswari; Singh, Hardeep

    2012-10-01

    On March 11, 2009, the Veterans Health Administration (VA) implemented an electronic health record (EHR)-based intervention that required all pathology results to be transmitted to ordering providers by mandatory automated notifications. We examined the impact of this intervention on improving follow-up of abnormal outpatient pathology results. We extracted pathology reports from the EHR of 2 VA sites. From 16,738 preintervention and 17,305 postintervention reports between 09/01/2008 and 09/30/2009, we randomly selected about 5% and evaluated follow-up outcomes using a standardized chart review instrument. Documented responses to the alerted report (eg, ordering follow-up tests or referrals, notifying patients, and prescribing/changing treatment) were recorded. Primary outcome measures included proportion of timely follow-up responses (within 30 d) and median time to direct response for abnormal reports. Of 816 preintervention and 798 postintervention reports reviewed, 666 (81.6%) and 688 (86.2%) were abnormal. Overall, there was no apparent intervention effect on timely follow-up (69% vs. 67.1%; P=0.4) or median time to direct response (8 vs. 8 d; P=0.7). However, logistic regression uncovered a significant intervention effect (preintervention odds ratio, 0.7; 95% confidence interval, 0.5-1.0) after accounting for site-specific differences in follow-up, with a lower likelihood of timely follow-up at one site (odds ratio, 0.4; 95% confidence interval, 0.2-0.7). An electronic intervention to improve test result follow-up at 2 VA institutions using the same EHR was found effective only after accounting for certain local contextual factors. Aggregating the effect of EHR interventions across different institutions and EHRs without controlling for contextual factors might underestimate their potential benefits.

  1. Complex conductivity results to silver nanoparticles in partically saturated laboratory columns

    Data.gov (United States)

    U.S. Environmental Protection Agency — Laboratory complex conductivity data from partially saturated sand columns with silver nanoparticles. This dataset is not publicly accessible because: It involves...

  2. Congenital oval or round window anomaly with or without abnormal facial nerve course: surgical results for 15 ears.

    NARCIS (Netherlands)

    Thomeer, H.G.; Kunst, H.P.; Verbist, B.M.; Cremers, C.W.R.J.

    2012-01-01

    OBJECTIVES: To describe the audiometric results in a consecutive series of patients with congenital ossicular aplasia (Class 4a) or dysplasia of the oval and/or round window (Class 4b), which might include a possible anomalous course of the facial nerve. STUDY DESIGN: Retrospective chart study.

  3. Measurement of advective soil gas flux: Results of field and laboratory experiments with CO2

    Energy Technology Data Exchange (ETDEWEB)

    Amonette, James E.; Barr, Jonathan L.; Erikson, Rebecca L.; Dobeck, Laura M.; Barr, Jamie L.; Shaw, Joseph A.

    2013-10-01

    We modified our multi-channel, steady-state flow-through (SSFT), soil-CO2 flux monitoring system to include an array of inexpensive pyroelectric non-dispersive infrared detectors for full-range (0-100%) coverage of CO2 concentrations without dilution, and a larger-diameter vent tube. We then conducted field testing of this system from late July through mid-September 2010 at the Zero Emissions Research and Technology (ZERT) project site located in Bozeman, MT, and subsequently, laboratory testing at the Pacific Northwest National Laboratory (PNNL) in Richland, WA using a flux bucket filled with dry sand. In the field, an array of twenty-five SSFT and three non-steady-state (NSS) flux chambers was installed in a 10x4 m area, the long boundary of which was directly above a shallow (2-m depth) horizontal injection well located 0.5 m below the water table. Two additional chambers (one SSFT and one NSS) were installed 10 m from the well for background measurements. Volumetric soil moisture sensors were installed at each SSFT chamber to measure mean levels in the top 0.15 m of soil. A total flux of 52 kg CO2 d-1 was injected into the well for 27 d and the efflux from the soil was monitored by the chambers before, during, and for 27 d after the injection. Overall, the results were consistent with those from previous years, showing a radial efflux pattern centered on a known “hot spot”, rapid responses to changes in injection rate and wind power, evidence for movement of the CO2 plume during the injection, and nominal flux levels from the SSFT chambers that were up to 6-fold higher than those measured by adjacent NSS chambers. Soil moisture levels varied during the experiment from moderate to near saturation with the highest levels occurring consistently at the hot spot. The effects of wind on measured flux were complex and decreased as soil moisture content increased. In the laboratory, flux bucket testing with the SSFT chamber showed large measured-flux enhancement

  4. Standardisation of environmental enrichment for laboratory mice and rats: Utilisation, practicality and variation in experimental results

    NARCIS (Netherlands)

    Baumans, V.; Loo, P.L.P. van; Pham, T.M.

    2010-01-01

    Rats and mice are the most commonly used species as laboratory animal models of diseases in biomedical research. Environmental factors such as cage size, number of cage mates and cage structure such as environmental enrichment can affect the physiology and behavioural development of laboratory anima

  5. Area Monitoring Dosimeter Program for the Pacific Northwest National Laboratory: Results for CY 1998

    Energy Technology Data Exchange (ETDEWEB)

    SR Bivins; GA Stoetzel

    1999-06-17

    In January 1993, Pacific Northwest National Laboratory (PNNL) established an area monitoring dosimeter program in accordance with Article 514 of the Department of Energy (DOE) Radiological Control Manual (RCM). The purpose of the program was to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological Buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a)(1)-(4) and Article 511.1 of the RCM, personnel dosimetry shall be provided to (1) radiological workers who are likely to receive at least 100 mrem annually and (2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years 1993-1997 confirmed that personnel dosimetry was not needed for individuals located in areas monitored by the program. A total of 97 area thermoluminescent dosimeters (TLDs) were placed in PNNL facilities during calendar year 1998. The TLDs were exchanged and analyzed quarterly. All routine area monitoring TLD results were less than 50 mrem annually after correcting for worker occupancy. The results support the conclusion that personnel dosimeters are not necessary for staff, declared pregnant workers, minors, or members of the public in these monitored areas.

  6. Area monitoring dosimeter program for the Pacific Northwest National Laboratory: Results for CY 1996

    Energy Technology Data Exchange (ETDEWEB)

    Bivins, S R; Stoetzel, G A

    1997-06-01

    In January 1993, Pacific Northwest National Laboratory (PNNL) established an area monitoring dosimeter program in accordance with Article 514 of the Department of Energy (DOE) Radiological Control Manual (RCM). The purpose of the program was to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a) (1)-(3) and Article 511.1 of the RCM, personnel dosimetry shall be provided to (1) radiological workers who are likely to receive at least 100 mrem annually and (2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years 1993, 1994, and 1995 confirmed that personnel dosimetry was not needed for individuals located in areas monitored by the program. A total of 108 area TLDs were placed in PNNL facilities during CY 1996. The TLDs were exchanged and analyzed quarterly. All routine area monitoring TLD results were less than 50 mrem annually after correcting for worker occupancy. The results support the conclusion that personnel dosimeters are not necessary for staff, declared pregnant workers, minors, or members of the public in these monitored areas.

  7. Area monitoring dosimeter program for the Pacific Northwest National Laboratory: Results for CY 1997

    Energy Technology Data Exchange (ETDEWEB)

    Bivins, S.R.; Stoetzel, G.A.

    1998-07-01

    In January 1993, Pacific Northwest National Laboratory (PNNL) established an area monitoring dosimeter program in accordance with Article 514 of the US Department of Energy (DOE) Radiological Control Manual (RCM). The purpose of the program was to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological Buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a) (1)--(3) and Article 511.1 of the RCM, personnel dosimetry shall be provided to (1) radiological workers who are likely to receive at least 100 mrem annually, and (2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years (CY) 1993--1996 confirmed that personnel dosimetry was not needed for individuals located in areas monitored by the program. A total of 93 area thermoluminescent dosimeters (TLDs) were placed in PNNL facilities during CY 1997. The TLDs were exchanged and analyzed quarterly. All routine area monitoring TLD results were less than 50 mrem annually after correcting for worker occupancy. The results support the conclusions that personnel dosimeters are not necessary for staff, declared pregnant workers, minors, or members of the public in these monitored areas.

  8. A splice site mutation in laminin-α2 results in a severe muscular dystrophy and growth abnormalities in zebrafish.

    Directory of Open Access Journals (Sweden)

    Vandana A Gupta

    Full Text Available Congenital muscular dystrophy (CMD is a clinically and genetically heterogeneous group of inherited muscle disorders. In patients, muscle weakness is usually present at or shortly after birth and is progressive in nature. Merosin deficient congenital muscular dystrophy (MDC1A is a form of CMD caused by a defect in the laminin-α2 gene (LAMA2. Laminin-α2 is an extracellular matrix protein that interacts with the dystrophin-dystroglycan (DGC complex in membranes providing stability to muscle fibers. In an N-ethyl-N-nitrosourea mutagenesis screen to develop zebrafish models of neuromuscular diseases, we identified a mutant fish that exhibits severe muscular dystrophy early in development. Genetic mapping identified a splice site mutation in the lama2 gene. This splice site is highly conserved in humans and this mutation results in mis-splicing of RNA and a loss of protein function. Homozygous lama2 mutant zebrafish, designated lama2(cl501/cl501, exhibited reduced motor function and progressive degeneration of skeletal muscles and died at 8-15 days post fertilization. The skeletal muscles exhibited damaged myosepta and detachment of myofibers in the affected fish. Laminin-α2 deficiency also resulted in growth defects in the brain and eye of the mutant fish. This laminin-α2 deficient mutant fish represents a novel disease model to develop therapies for modulating splicing defects in congenital muscular dystrophies and to restore the muscle function in human patients with CMD.

  9. Leukocyte abnormalities.

    Science.gov (United States)

    Gabig, T G

    1980-07-01

    Certain qualitative abnormalities in neutrophils and blood monocytes are associated with frequent, severe, and recurrent bacterial infections leading to fatal sepsis, while other qualitative defects demonstrated in vitro may have few or no clinical sequelae. These qualitative defects are discussed in terms of the specific functions of locomotion, phagocytosis, degranulation, and bacterial killing.

  10. Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities.

    Science.gov (United States)

    Mahoney, My G; Sadowski, Sara; Brennan, Donna; Pikander, Pekka; Saukko, Pekka; Wahl, James; Aho, Heikki; Heikinheimo, Kristiina; Bruckner-Tuderman, Leena; Fertala, Andrzej; Peltonen, Juha; Uitto, Jouni; Peltonen, Sirkku

    2010-04-01

    Desmoplakin (DP) anchors the intermediate filament cytoskeleton to the desmosomal cadherins and thereby confers structural stability to tissues. In this study, we present a patient with extensive mucocutaneous blisters, epidermolytic palmoplantar keratoderma, nail dystrophy, enamel dysplasia, and sparse woolly hair. The patient died at the age of 14 years from undiagnosed cardiomyopathy. The skin showed hyperplasia and acantholysis in the mid- and lower epidermal layers, whereas the heart showed extensive fibrosis and fibrofatty replacement in both ventricles. Immunofluorescence microscopy showed a reduction in the C-terminal domain of DP in the skin and oral mucosa. Sequencing of the DP gene showed undescribed mutations in the maternal and paternal alleles. Both mutations affected exon 24 encoding the C-terminal domain. The paternal mutation, c.6310delA, leads to a premature stop codon. The maternal mutation, c.7964 C to A, results in a substitution of an aspartic acid for a conserved alanine residue at amino acid 2655 (A2655D). Structural modeling indicated that this mutation changes the electrostatic potential of the mutated region of DP, possibly altering functions that depend on intermolecular interactions. To conclude, we describe a combination of DP mutation phenotypes affecting the skin, heart, hair, and teeth. This patient case emphasizes the importance of heart examination of patients with desmosomal genodermatoses.

  11. Instability of surface lenticular vortices: results from laboratory experiments and numerical simulations

    Science.gov (United States)

    Lahaye, Noé; Paci, Alexandre; Smith, Stefan Llewellyn

    2016-04-01

    We examine the instability of lenticular vortices -- or lenses -- in a stratified rotating fluid. The simplest configuration is one in which the lenses overlay a deep layer and have a free surface, and this can be studied using a two-layer rotating shallow water model. We report results from laboratory experiments and high-resolution direct numerical simulations of the destabilization of vortices with constant potential vorticity, and compare these to a linear stability analysis. The stability properties of the system are governed by two parameters: the typical upper-layer potential vorticity and the size (depth) of the vortex. Good agreement is found between analytical, numerical and experimental results for the growth rate and wavenumber of the instability. The nonlinear saturation of the instability is associated with conversion from potential to kinetic energy and weak emission of gravity waves, giving rise to the formation of coherent vortex multipoles with trapped waves. The impact of flow in the lower layer is examined. In particular, it is shown that the growth rate can be strongly affected and the instability can be suppressed for certain types of weak co-rotating flow.

  12. Students' Progression in Monitoring Anomalous Results Obtained in Inquiry-Based Laboratory Tasks

    Science.gov (United States)

    Crujeiras-Pérez, Beatriz; Jiménez-Aleixandre, Maria Pilar

    2017-07-01

    This paper examines students' engagement in monitoring anomalous results across a 2-year longitudinal study with 9th and 10th graders (14-15 and 15-16 years of age). The context is a set of five inquiry-based laboratory tasks, requiring students to plan and carry out investigations. The study seeks to examine students' interpretation of data, in particular anomalous results generated by them during the process of solving the tasks, and their ability to monitor them. Data collected include video and audio recordings as well as students' written products. For the analysis, two rubrics were developed drawing on Chinn and Brewer (Cognition and Instruction, 19, 323-393, 2001) and Hmelo-Silver et al. (Science Education, 86, 219-243, 2002). The findings point to a pattern of progress in students' responses across the 2 years: (a) responses revealing a low capacity of monitoring due to not recognizing the data as anomalous or recognizing it as anomalous but being unable to explain their causes are more frequent in the first tasks and (b) responses revealing an improved capacity of monitoring are more frequent in the last tasks. The factors influencing students' regulation of their performances, as the requirement of planning, and specific scaffolding based on activity theory are discussed.

  13. Providing critical laboratory results on time, every time to help reduce emergency department length of stay: how our laboratory achieved a Six Sigma level of performance.

    Science.gov (United States)

    Blick, Kenneth E

    2013-08-01

    To develop a fully automated core laboratory, handling samples on a "first in, first out" real-time basis with Lean/Six Sigma management tools. Our primary goal was to provide services to critical care areas, eliminating turnaround time outlier percentage (TAT-OP) as a factor in patient length of stay (LOS). A secondary goal was to achieve a better laboratory return on investment. In 2011, we reached our primary goal when we calculated the TAT-OP distribution and found we had achieved a Six Sigma level of performance, ensuring that our laboratory service can be essentially eliminated as a factor in emergency department patient LOS. We also measured return on investment, showing a productivity improvement of 35%, keeping pace with our increased testing volume. As a result of our Lean process improvements and Six Sigma initiatives, in part through (1) strategic deployment of point-of-care testing and (2) core laboratory total automation with robotics, middleware, and expert system technology, physicians and nurses at the Oklahoma University Medical Center can more effectively deliver lifesaving health care using evidence-based protocols that depend heavily on "on time, every time" laboratory services.

  14. Electromagnetic (EM) earthquake precursor transmission and detection regarding experimental field and laboratory results.

    Science.gov (United States)

    Jones, Kenneth B., II; Saxton, Patrick

    2016-04-01

    Aside from understanding the animal kingdom reacting to a per-earthquake signal, a transmission source is apparent. The focus of this investigation is an electromagnetic emission approach and detection capable of becoming both practical and reliable to other plausible earthquake precursors. To better determine this method, several prototype magnetometers were devised and built with each successive version improving upon the next. Two twin (prototype #2) antennae were deployed to field settings outside the NE Texas town of Timpson, TX back in February, 2013 and very recent laboratory tests using the most refined (prototype #4) experimental antenna for detecting unconfined, granitic block fracturing. Field testing encompassed the small NE Texas town of Timpson, TX, which endured an earthquake phenomenon (May, 2012 - September, 2013). A rare sequence of events was strictly attributed to hydraulic fracturing activity in the immediate area all for hydrocarbon capture; thus, a chance to detect and record man-made earthquake activity. By swiveling two directional antennae at three locations, one mobile, the antennae could 'zero' in on a signal source until its pattern was well established and mapped, accordingly. Three signals were detected, two strong and one moderately strong, each with epicenter implications several kilometers from known seismological sites. Six months later, two M4s and a M2.4 earthquake hit over the 2013 Labor Day weekend. Hydraulic pump pressure increased deep Earth pore pressure, reduced friction, and displaced opposing tectonic stresses causing rock to fracture. This was the last earthquake sequence in the Timpson area, due to personal involvement and area citizens in contact with their state representatives. Well and drilling operations have since moved 40-50 miles SE of Timpson, TX and rare earthquake activity has now occurred there. Laboratory testing was next performed using cored granitic blocks and the latest, improved antenna with an

  15. Role of Organic Acids in Bioformation of Kaolinite: Results of Laboratory Experiments

    Science.gov (United States)

    Bontognali, T. R. R.; Vasconcelos, C.; McKenzie, J. A.

    2012-04-01

    Clay minerals and other solid silica phases have a broad distribution in the geological record and greatly affect fundamental physicochemical properties of sedimentary rocks, including porosity. An increasing number of studies suggests that microbial activity and microbially produced organic acids might play an important role in authigenic clay mineral formation, at low temperatures and under neutral pH conditions. In particular, early laboratory experiments (Linares and Huertas, 1971) reported the precipitation of kaolinite in solutions of SiO2 and Al2O3 with different molar ratios SiO2/Al2O3, together with fulvic acid (a non-characterized mixture of many different acids containing carboxyl and phenolate groups) that was extracted from peat soil. Despite many attempts, these experiments could not be reproduced until recently. Fiore et al. (2011) hypothesized that the non-sterile fulvic acid might have contained microbes that participated in the formation of kaolinite. Using solutions saturated with Si and Al and containing oxalate and/or mixed microbial culture extracted from peat-moss soil, they performed incubation experiments, which produced kaolinite exclusively in solutions containing oxalate and microbes. We proposed to test the role of specific organic acids for kaolinite formation, conducting laboratory experiments at 25˚C, with solutions of sodium silicate, aluminum chloride and various organic compounds (i.e. EDTA, citric acid, succinic acid and oxalic acid). Specific organic acids may stabilize aluminum in octahedral coordination positions, which is crucial for the initial nucleation step. In our experiments, a poorly crystalline mineral that is possibly a kaolinite precursor formed exclusively in the presence of succinic acid. In experiments with other organic compounds, no incorporation of Al was observed, and amorphous silica was the only precipitated phase. In natural environments, succinic acid is produced by a large variety of microbes as an

  16. IMAGE Project: Results of Laboratory Tests on Tracers for Supercritical Conditions.

    Science.gov (United States)

    Brandvoll, Øyvind; Opsahl Viig, Sissel; Nardini, Isabella; Muller, Jiri

    2016-04-01

    The use of tracers is a well-established technique for monitoring dynamic behaviour of water and gas through a reservoir. In geothermal reservoirs special challenges are encountered due to high temperatures and pressures. In this work, tracer candidates for monitoring water at supercritical conditions (temperature > 374°C, pressure ca 218 bar), are tested in laboratory experiments. Testing of tracers at supercritical water conditions requires experimental set-ups which tolerate harsh conditions with respect to high temperature and pressure. In addition stringent HES (health, environment and safety) factors have to be taken into consideration when designing and performing the experiments. The setup constructed in this project consists of a pressure vessel, high pressure pump, instrumentation for pressure and temperature control and instrumentation required for accurate sampling of tracers. In order to achieve accurate results, a special focus has been paid to the development of the tracer sampling technique. Perfluorinated cyclic hydrocarbons (PFCs) have been selected as tracer candidates. This group of compounds is today commonly used as gas tracers in oil reservoirs. According to the literature they are stable at temperatures up to 400°C. To start with, five PFCs have been tested for thermal stability in static experiments at 375°C and 108 bar in the experimental setup described above. The tracer candidates will be further tested for several months at the relevant conditions. Preliminary results indicate that some of the PFC compounds show stability after three months. However, in order to arrive at conclusive results, the experiments have to be repeated over a longer period and paying special attention to more accurate sampling procedures.

  17. Comparative assay of fluorescent antibody test results among twelve European National Reference Laboratories using various anti-rabies conjugates

    DEFF Research Database (Denmark)

    Robardet, E.; Andrieu, S.; Rasmussen, Thomas Bruun

    2013-01-01

    Twelve National Reference Laboratories (NRLs) for rabies have undertaken a comparative assay to assess the comparison of fluorescent antibody test (FAT) results using five coded commercial anti-rabies conjugates (Biorad, Bioveta, Fujirebio, Millipore, and SIFIN conjugates). Homogenized positive...

  18. Prevalence of asymptomatic urinary abnormalities among adolescents

    Directory of Open Access Journals (Sweden)

    Mohamed Fouad

    2016-01-01

    Full Text Available To determine the prevalence of asymptomatic urinary abnormalities in adolescents, first morning clean mid-stream urine specimens were obtained from 2500 individuals and examined by dipstick and light microscopy. Adolescents with abnormal screening results were reexamined after two weeks and those who had abnormal results twice were subjected to systemic clinical examination and further clinical and laboratory investigations. Eight hundred and three (32.1% individuals had urinary abnormalities at the first screening, which significantly decreased to 345 (13.8% at the second screening, (P <0.001. Hematuria was the most common urinary abnormalities detected in 245 (9.8% adolescents who had persistent urine abnormalities; 228 (9.1% individuals had non glomerular hematuria. The hematuria was isolated in 150 (6% individuals, combined with leukocyturia in 83 (3.3% individuals, and combined with proteinuria in 12 (0.5% individuals. Leukocyturia was detected in 150 (6% of all studied adolescents; it was isolated in 39 (1.6% individuals and combined with proteinuria in 28 (1.1% of them. Asymp- tomatic bacteriuria was detected in 23 (0.9% of all studied adolescents; all the cases were females. Proteinuria was detected in 65 (2.6% of all the studied adolescents; 45 (1.8% indivi- duals had <0.5 g/day and twenty (0.8% individuals had 0.5-3 g/day. Asymptomatic urinary abnormalities were more common in males than females and adolescents from rural than urban areas (P <0.01 and (P <0.001, respectively. The present study found a high prevalence of asymptomatic urinary abnormalities among adolescents in our population.

  19. The role of laboratory dissection training in neurosurgical residency: results of a national survey.

    Science.gov (United States)

    Kshettry, Varun R; Mullin, Jeffrey P; Schlenk, Richard; Recinos, Pablo F; Benzel, Edward C

    2014-11-01

    Work hour restrictions and current quality, financial, and legal concerns have reduced resident operative volume and autonomy. Although laboratory (cadaveric or animal) dissection has a rich history in neurosurgery, its current role in resident training is unclear. Recent literature suggests educators have looked to simulation to accelerate the learning curve of acquiring neurosurgical technical skills. The purpose of this study was to determine the prevalence, characteristics, and extent of laboratory dissection in neurosurgical residency programs in the United States. A survey was sent to program directors of all 100 neurosurgical residency programs in the United States. Response rate was 65%. Most programs (93.8%) incorporate laboratory dissection into resident training. Most programs have 1-3 (36.1%) or 4-6 (39.3%) sessions annually. Residents in postgraduate years 2-6 (85.2%-93.4%) most commonly participate. The most common topics are cranial approaches (100%), spinal approaches (88.5%), spine instrumentation (80.3%), and endoscopy (50.8%). Thirty-one (47.7%) programs use artificial physical model or virtual reality simulators; the most common simulators are endoscopy (15.4%), microvascular anastomosis (13.8%), and endovascular (10.8%). Only 8 programs (13.1%) formally grade dissection skills. Educators (95.4%) believe laboratory dissection is an integral component of training and no respondent believed simulation could currently provide greater educational benefit than laboratory dissection. Most (89.2%) respondents would support a national "suggested" dissection curriculum and manual. In neurosurgical resident education, laboratory dissection is widely used; however, significant variation exists. Nonetheless, program directors believe laboratory dissection plays an integral role in neurosurgical training and is currently associated with greater educational benefit than simulation. Copyright © 2014 Elsevier Inc. All rights reserved.

  20. Shaping the PSF to nearly top-hat profile: CHEOPS laboratory results

    Science.gov (United States)

    Magrin, Demetrio; Farinato, Jacopo; Umbriaco, Gabriele; Kumar Radhakrishnan Santhakumari, Kalyan; Bergomi, Maria; Dima, Marco; Greggio, Davide; Marafatto, Luca; Ragazzoni, Roberto; Viotto, Valentina; Munari, Matteo; Pagano, Isabella; Scandariato, Gaetano; Scuderi, Salvatore; Piotto, Giampaolo; Beck, Thomas; Benz, Willy; Broeg, Christopher; Cessa, Virginie; Fortier, Andrea; Piazza, Daniele

    2014-08-01

    Spreading the PSF over a quite large amount of pixels is an increasingly used observing technique in order to reach extremely precise photometry, such as in the case of exoplanets searching and characterization via transits observations. A PSF top-hat profile helps to minimize the errors contribution due to the uncertainty on the knowledge of the detector flat field. This work has been carried out during the recent design study in the framework of the ESA small mission CHEOPS. Because of lack of perfect flat-fielding information, in the CHEOPS optics it is required to spread the light of a source into a well defined angular area, in a manner as uniform as possible. Furthermore this should be accomplished still retaining the features of a true focal plane onto the detector. In this way, for instance, the angular displacement on the focal plane is fully retained and in case of several stars in a field these look as separated as their distance is larger than the spreading size. An obvious way is to apply a defocus, while the presence of an intermediate pupil plane in the Back End Optics makes attractive to introduce here an optical device that is able to spread the light in a well defined manner, still retaining the direction of the chief ray hitting it. This can be accomplished through an holographic diffuser or through a lenslet array. Both techniques implement the concept of segmenting the pupil into several sub-zones where light is spread to a well defined angle. We present experimental results on how to deliver such PSF profile by mean of holographic diffuser and lenslet array. Both the devices are located in an intermediate pupil plane of a properly scaled laboratory setup mimicking the CHEOPS optical design configuration.

  1. Thermo-Hydro-Mechanical properties of MX-80. Results from advanced laboratory tests

    Energy Technology Data Exchange (ETDEWEB)

    Dueck, Ann; Nilsson, Ulf (Clay Technology AB, Lund (Sweden))

    2010-12-15

    General Highly compacted bentonite is proposed as the buffer material in the Swedish concept for disposal of nuclear waste. The saturated homogenized bentonite is expected to fully act as a buffer material between the waste canister and the surrounding bedrock. Material models describing the thermo-hydro-mechanical (THM) behaviour of the buffer material have been created with the purpose to simulate and predict the behaviour in a repository both before and after water saturation. The material models of water saturated and water unsaturated buffer material are complicated and contain a number of parameters that need to be determined. The present report is a compilation of results concerning thermo-hydro-mechanical laboratory tests on saturated and unsaturated buffer material. The main purpose of the report is to supply modelling groups with available results for improving models and determine parameters that can be used for the THM modelling of the behaviour of the buffer.Retention curves The relation between water content and relative humidity has been determined in a number of test series for some specific conditions, e.g. different initial water contents. Two methods have been used; the sorption balance method and a method with jars as desiccators. The majority of the results were derived from tests where RH was controlled and the response of the bentonite samples was measured. The results are given as water content versus relative humidity in diagrams and in tabular form. Volume change The volume change of water unsaturated bentonite specimens has been investigated by compression tests and swelling/shrinkage tests for some specific stress and moisture paths. The constant relative humidity was generated by the vapour equilibrium technique combined with an air circulation system. Measured stresses, deformation and relative humidity are presented versus time in diagrams and the final values at different stages are also presented in tabular form. Moisture transport

  2. Real life turnaround time of blood cultures in the clinical microbiology laboratory: results of the first Italian survey, May 2015

    Directory of Open Access Journals (Sweden)

    Fabio Arena

    2016-10-01

    Full Text Available Background and aims: Blood culture (BC results are essential to guide antimicrobial chemotherapy for patients with sepsis. However, BC is a time-consuming exam, which can take several days. Reducing BCs turn around time (TAT could impact on multiple outcome parameters and TAT monitoring is an important tool for measurement of microbiology laboratory performance. The aim of this study was to provide an overview of BC TATs among Italian microbiology laboratories. Materials and methods: Five laboratories collected and recorded, for a month period, date and time of the BC processing events. Cumulative TATs were analysed using the GraphPad software. Results: Participating laboratories reported data from 302 sepsis episodes. The median time from when the BC system produced a positive signal until Gram-stain results were reported was 7.6 hours. A rapid molecular identification and antimicrobial susceptibility testing (AST was performed in 26.5% of BCs. Mean TAT for identification report was significantly lower when a molecular approach was adopted (12 vs. 28.7 hours, P<0.001. Similarly, results of the molecular AST were obtained more than 24 hours in advance compared with phenotypic AST (mean 13.2 vs. 47.6, P<0.001. TATs from BC positivity of laboratories opened 7 days/week were not significantly lower than those of laboratories opened 6 days/week. Conclusions: BC is a time-consuming exam, however, molecular identification and AST methods can drastically reduce time to results. The lack of difference between TATs observed for laboratories working 7 days/week and 6 days/week, coupled with a high rate of BCs turning positive during the night enable to conclude that the most urgent measure to reduce TATs is the expansion of laboratory regular duty hours.

  3. Chemical characterization of clay SRM by X-ray fluorescence – results comparison from different laboratories

    Directory of Open Access Journals (Sweden)

    Carlos Roberto Appoloni

    2009-03-01

    Full Text Available Two Energy Dispersive X-ray Fluorescence equipments have been compared in order to verify its performance for clay characterization and potential application to the study in archaeometric field and industry. Two clay standard reference materials (SRM, IPT-42 and IPT-51 and one IAEA intercomparison sample were analyzed by two different methodologies and equipments. Asentamiento Universitario Zapala laboratory has a Shimadzu EDX-800HS bench top equipment and 13 elements from S to Zr were quantified in the standards. Applied Nuclear Physics Laboratory has a portable EDXRF system. It was possible to quantify K, Ti and Fe and qualitatively to identify Mn, Rb, Zn and Zr.

  4. X-ray diffraction results from mars science laboratory: Mineralogy of rocknest at Gale crater

    NARCIS (Netherlands)

    Bish, D.L.; Blake, D.F.; Vaniman, D.T.; Chipera, S.J.; Morris, R.V.; Ming, D.W.; Treiman, A.H.; Sarrazin, P.; Morrison, S.M.; Downs, R.T.; Achilles, C.N.; Yen, A.S.; Bristow, T.F.; Crisp, J.A.; Morookian, J.M.; Farmer, J.D.; Rampe, E.B.; Stolper, E.M.; Spanovich, N.; MSL Science Team, the

    2013-01-01

    The Mars Science Laboratory rover Curiosity scooped samples of soil from the Rocknest aeolian bedform in Gale crater. Analysis of the soil with the Chemistry and Mineralogy (CheMin) x-ray diffraction (XRD) instrument revealed plagioclase (~An57), forsteritic olivine (~Fo62), augite, and pigeonite, w

  5. Model-Based Reasoning in the Physics Laboratory: Framework and Initial Results

    Science.gov (United States)

    Zwickl, Benjamin M.; Hu, Dehui; Finkelstein, Noah; Lewandowski, H. J.

    2015-01-01

    We review and extend existing frameworks on modeling to develop a new framework that describes model-based reasoning in introductory and upper-division physics laboratories. Constructing and using models are core scientific practices that have gained significant attention within K-12 and higher education. Although modeling is a broadly applicable…

  6. Melatonin prevents abnormal mitochondrial dynamics resulting from the neurotoxicity of cadmium by blocking calcium-dependent translocation of Drp1 to the mitochondria.

    Science.gov (United States)

    Xu, Shangcheng; Pi, Huifeng; Zhang, Lei; Zhang, Nixian; Li, YuMing; Zhang, Huiliang; Tang, Ju; Li, Huijuan; Feng, Min; Deng, Ping; Guo, Pan; Tian, Li; Xie, Jia; He, Mindi; Lu, Yonghui; Zhong, Min; Zhang, Yanwen; Wang, Wang; Reiter, Russel J; Yu, Zhengping; Zhou, Zhou

    2016-04-01

    Cadmium (Cd) is a persistent environmental toxin and occupational pollutant that is considered to be a potential risk factor in the development of neurodegenerative diseases. Abnormal mitochondrial dynamics are increasingly implicated in mitochondrial damage in various neurological pathologies. The aim of this study was to investigate whether the disturbance of mitochondrial dynamics contributed to Cd-induced neurotoxicity and whether melatonin has any neuroprotective properties. After cortical neurons were exposed to 10 μM cadmium chloride (CdCl2 ) for various periods (0, 3, 6, 12, and 24 hr), the morphology of their mitochondria significantly changed from the normal tubular networks into punctuated structures within 3 hr. Following this pronounced mitochondrial fragmentation, Cd treatment led to signs of mitochondrial dysfunction, including excess reactive oxygen species (ROS) production, decreased ATP content, and mitochondrial membrane potential (▵Ψm) loss. However, 1 mM melatonin pretreatment efficiently attenuated the Cd-induced mitochondrial fragmentation, which improved the turnover of mitochondrial function. In the brain tissues of rats that were intraperitoneally given 1 mg/kg CdCl2 for 7 days, melatonin also ameliorated excessive mitochondrial fragmentation and mitochondrial damage in vivo. Melatonin's protective effects were attributed to its roles in preventing cytosolic calcium ([Ca(2+) ]i ) overload, which blocked the recruitment of Drp1 from the cytoplasm to the mitochondria. Taken together, our results are the first to demonstrate that abnormal mitochondrial dynamics is involved in cadmium-induced neurotoxicity. Melatonin has significant pharmacological potential in protecting against the neurotoxicity of Cd by blocking the disbalance of mitochondrial fusion and fission.

  7. LABORATORY TESTING RESULTS ANALYSIS OF GIARDIASIS PREVALENCE AMONG DIFFERENT POPULATION GROUPS IN THE NENETS AUTONOMOUS DISTRICT

    Directory of Open Access Journals (Sweden)

    N. S. Bobyreva

    2015-01-01

    Full Text Available The Nenets Autonomous District (NAD refers to the territories of the Russia’s Far North. The country is the only region of Russia, where there are still no roads. The extreme weather conditions, as well as residential patterns in the NAD reduce the availability of specialized medical care, as indigenous people and other nationalities living in the district, which requires the use of health care management specific forms. The article presents data on giardiasis studies amongst different categories of the NAD population, obtained as a result of the population surveys in remote regions of the Russian Federation Arctic zone. Samples studied were serum and faecal emulsion. The study used the following laboratory methods: for serum — Giardia lamblia antibody screening method using ELISA kit, emulsion stool — native smear microscopy analysis, the method of enrichment using disposable concentrators «Parasep» immunological diagnostic methods based on the detection of specific lamblia antigen in faeces using ELISA kits. For statistics was used descriptive statistics analysis (mean values, the percentage error of the mean, the construction of the trend line with program SPSS 20.00, Excel 2010, the method of analysis of official statistical reporting hospitals survey on giardiasis, data of Rospotrebnadzor (Russian Sanitary service for NAD and data from own research from 2002 to 2013. Just in the process of research carried out research on the 10 356 giardiasis, registered diseases by 3470 cases of giardiasis, accounting for 99.14% of the total number of protozoonosises registered and 37.4% of all registered in the NAD parasitosis. The comparison was made among the population of the county towns, home to both the indigenous people, nomadic, and representatives of other ethnic groups, sedentary with urban population of the city of Naryan-Mar. The article analyzes the incidence rates for giardiasis according to district official statistical reporting

  8. [Diagnosis of infections caused by Clostridium difficile in the Czech Republic: availability, possibilities, and interpretation of laboratory results].

    Science.gov (United States)

    Krůtová, M; Nyč, O

    2015-06-01

    To assess the availability of the laboratory diagnosis of infections caused by C. difficile in the Czech Republic (CR), including the range of tests used, possible combinations, and adequate interpretation of model results. Data were collected through a web questionnaire survey with the participation of representatives of 61 public and private microbiological laboratories. The questionnaire addressed the use of diagnostic test kits and culture media in the diagnosis of C. difficile infection (CDI). In addition, the respondents were asked to interpret a glutamate dehydrogenase (GDH) positive and, at the same time, toxin A/B negative result, without or with laboratory confirmation if available. In the CR, the most commonly used test in the diagnosis of CDI is the C. DIFF Quik Chek Complete® test (Alere) for the detection of GDH and A/B toxins, as reported by 50 (82%) laboratories. Anaerobic culture is performed by 43 (70.5%) laboratories, 21 (48.8%) of which use selective agar (Oxoid). Direct detection of DNA of toxigenic C. difficile is feasible in 17 (27.9%) laboratories, with most of them (15 laboratories) using the closed system Xpert® C. difficile (Cepheid). The diagnosis based only on the detection of GDH and A/B toxins is used by 13 (21.3%) laboratories. Two (3.3%) laboratories detect A/B toxins alone and three (4.9%) laboratories carry out the detection of A/B toxins followed by anaerobic culture. A three step scheme: detection of GDH and A/B toxins with subsequent anaerobic culture is used by 26 (42.6%) laboratories. The detection of GDH and A/B toxins along with a PCR assay are provided by three (4.9%) laboratories. A complete diagnostic scheme for CDI (detection of GDH and A/B toxins, direct detection of DNA, and aerobic culture) is feasible in 14 (23%) laboratories. This questionnaire survey study identified 24 different testing algorithms to be in use within the study period (April to July 2014) in the CR. Five (8.2%) laboratories have no highly

  9. Cold Climate Foundation Retrofit Experimental Hygrothermal Performance. Cloquet Residential Research Facility Laboratory Results

    Energy Technology Data Exchange (ETDEWEB)

    Goldberg, Louise F. [NorthernSTAR, St. Paul, MN (United States); Harmon, Anna C. [NorthernSTAR, St. Paul, MN (United States)

    2015-04-09

    This project was funded jointly by the National Renewable Energy Laboratory (NREL) and Oak Ridge National Laboratory (ORNL). ORNL focused on developing a full basement wall system experimental database to enable others to validate hygrothermal simulation codes. NREL focused on testing the moisture durability of practical basement wall interior insulation retrofit solutions for cold climates. The project has produced a physically credible and reliable long-term hygrothermal performance database for retrofit foundation wall insulation systems in zone 6 and 7 climates that are fully compliant with the performance criteria in the 2009 Minnesota Energy Code. These data currently span the period from November 10, 2012 through May 31, 2014 and are anticipated to be extended through November 2014. The experimental data were configured into a standard format that can be published online and that is compatible with standard commercially available spreadsheet and database software.

  10. Costs and benefits of cold acclimation in field released Drosophila – Associating laboratory and field results.

    DEFF Research Database (Denmark)

    Overgaard, Johannes; Sørensen, Jesper Givskov; A. Hoffmann, Ary

    2008-01-01

    Physiological and evolutionary responses to thermal variation are often investigated under controlled laboratory conditions. However, this approach may fail to account for the complexity of natural environments. Here we investigated the costs and benefits of developmental or adult cold acclimation...... temperatures where cold acclimated flies were up to 36 times less likely to find a resource under warm conditions. These costs were not detected in standard laboratory tests but indicate that physiological acclimation may improve fitness only over a narrow set of thermal conditions while it may have...... that the ability to locate a field resource has a genetic basis with a high heritability since only round of selection on parental flies (F0) revealed clear differences in the ability of offspring (F1 and F2) to locate field resources at cold temperatures. Again we found a poor association between field...

  11. Prevalence of asymptomatic urinary abnormalities among adolescents.

    Science.gov (United States)

    Fouad, Mohamed; Boraie, Maher

    2016-05-01

    To determine the prevalence of asymptomatic urinary abnormalities in adolescents, first morning clean mid-stream urine specimens were obtained from 2500 individuals and examined by dipstick and light microscopy. Adolescents with abnormal screening results were reexamined after two weeks and those who had abnormal results twice were subjected to systemic clinical examination and further clinical and laboratory investigations. Eight hundred and three (32.1%) individuals had urinary abnormalities at the first screening, which significantly decreased to 345 (13.8%) at the second screening, (P adolescents who had persistent urine abnormalities; 228 (9.1%) individuals had non glomerular hematuria. The hematuria was isolated in 150 (6%) individuals, combined with leukocyturia in 83 (3.3%) individuals, and combined with proteinuria in 12 (0.5%) individuals. Leukocyturia was detected in 150 (6%) of all studied adolescents; it was isolated in 39 (1.6%) individuals and combined with proteinuria in 28 (1.1%) of them. Asymptomatic bacteriuria was detected in 23 (0.9%) of all studied adolescents; all the cases were females. Proteinuria was detected in 65 (2.6%) of all the studied adolescents; 45 (1.8%) individuals had adolescents from rural than urban areas (P adolescents in our population.

  12. The preliminary results of fast neutron flux measurements in the DULB laboratory at Baksan

    OpenAIRE

    2000-01-01

    One of the main sources of a background in underground physics experiments (such as the investigation of solar neutrino flux, neutrino oscillations, neutrinoless double beta decay, and the search for annual and daily Cold Dark Matter particle flux modulation) are fast neutrons originating from the surrounding rocks. The measurements of fast neutron flux in the new DULB Laboratory situated at a depth of 4900 m w.e. in the Baksan Neutrino Observatory have been performed. The relative neutron sh...

  13. The Results of a Laboratory Feasibility Study for the Biological Treatment of Umatilla Groundwater

    Science.gov (United States)

    2012-01-01

    Amendments. Amendment Supplier Contact Phone Address Lactose Columbia River Processing Roy Dugan 541·481-3770 79588 Rippee Road Whey Columbia River...degradation. RDX, on the other hand, was more difficult to treat. The best amendments were molasses, corn syrup, emulsified oil (EOS), lactose, and whey ...Environmental Processes and Engineering Division (EPED), Environmental Laboratory (EL). Dr. Fiona Crocker and Dr. Mansour Zakakhani provided in-house review

  14. Results of Laboratory Scale Fracture Tests on Rock/Cement Interfaces

    Energy Technology Data Exchange (ETDEWEB)

    Um, Wooyong; Jung, Hun Bok

    2012-06-01

    A number of pure cement and cement-basalt interface samples were subjected to a range of compressive loads to form internal fractures. X-ray microtomography was used to visualize the formation and growth of internal fractures in three dimensions as a function of compressive loads. This laboratory data will be incorporated into a geomechanics model to predict the risk of CO2 leakage through wellbores during geologic carbon storage.

  15. Factors controlling short-term soil microbial response after laboratory heating. Preliminary results

    Science.gov (United States)

    Jiménez-Compán, Elizabeth; Jiménez-Morillo, Nicasio; Jordán, Antonio; Bárcenas-Moreno, Gema

    2015-04-01

    Soil microbial response after fire is controlled by numerous variables which conclude with a mosaic of results depending on organic carbon alterations or pH fire-induced changes. This fact has complicated the studies focused on post-fire microbial response, compiling high variability of opposite result in the bibliography. Soil laboratory heating cannot emulate a real wildfire effect on soil but lead us the possibility to control several variables and it is a valid tool to clarify the relative weight of different factors controlling microbial response after soil heating. In this preliminary study different heated treatments were applied to unaltered forest soil samples, obtaining 4 different heating treatments to simulate a range of fire intensities: unaltered-control (UH), and soil heated at 300, 450 and 500 °C. In order to isolate possible nutrient availability or pH heating-induced changes, different culture media were prepared using soil:water extract from each heating treatments and adding different supplements to obtain the total of 11 different culture media: unheated soil without supplements (UH-N-), unheated soil with nutrient supplement (UH-N+), soil heated at 300 °C without supplements (300-N-), soil heated at 300 °C with nutrient supplement (300-N+), soil heated at 300 °C with nutrient supplement and pH-buffered (300-N+pH); soil heated at 450 °C without supplements (450-N-), soil heated at 450 °C with nutrient supplement (450-N+), soil heated at 450 °C with nutrient supplement and pH-buffered (450-N+); soil heated at 500 °C without supplements (500-N-), soil heated at 500 °C with nutrient supplement (500-N+), soil heated at 500 °C with nutrient supplement and pH-buffered (500-N+). Each media was inoculated with different dilutions of a microbial suspension from the original unaltered soil, and the abundance of viable and cultivable microorganisms were measured by plate count method. In addition, the analysis of heating-induced soil organic

  16. Comparison of blood gas, electrolyte and metabolite results measured with two different blood gas analyzers and a core laboratory analyzer.

    Science.gov (United States)

    Uyanik, Metin; Sertoglu, Erdim; Kayadibi, Huseyin; Tapan, Serkan; Serdar, Muhittin A; Bilgi, Cumhur; Kurt, Ismail

    2015-04-01

    Blood gas analyzers (BGAs) are important in assessing and monitoring critically ill patients. However, the random use of BGAs to measure blood gases, electrolytes and metabolites increases the variability in test results. Therefore, this study aimed to investigate the correlation of blood gas, electrolyte and metabolite results measured with two BGAs and a core laboratory analyzer. A total of 40 arterial blood gas samples were analyzed with two BGAs [(Nova Stat Profile Critical Care Xpress (Nova Biomedical, Waltham, MA, USA) and Siemens Rapidlab 1265 (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA)) and a core laboratory analyzer [Olympus AU 2700 autoanalyzer (Beckman-Coulter, Inc., Fullerton, CA, USA)]. The results of pH, pCO₂, pO₂, SO₂, sodium (Na⁺), potassium (K⁺), calcium (Ca⁺²), chloride (Cl⁻), glucose, and lactate were compared by Passing-Bablok regression analysis and Bland-Altman plots. The present study showed that there was negligible variability of blood gases (pCO₂, pO₂, SO₂), K⁺ and lactate values between the blood gas and core laboratory analyzers. However, the differences in pH were modest, while Na⁺, Cl⁻, Ca²⁺ and glucose showed poor correlation according to the concordance correlation coefficient. BGAs and core laboratory autoanalyzer demonstrated variable performances and not all tests met minimum performance goals. It is important that clinicians and laboratories are aware of the limitations of their assays.

  17. Variability of ethics education in laboratory medicine training programs: results of an international survey.

    Science.gov (United States)

    Bruns, David E; Burtis, Carl A; Gronowski, Ann M; McQueen, Matthew J; Newman, Anthony; Jonsson, Jon J

    2015-03-10

    Ethical considerations are increasingly important in medicine. We aimed to determine the mode and extent of teaching of ethics in training programs in clinical chemistry and laboratory medicine. We developed an on-line survey of teaching in areas of ethics relevant to laboratory medicine. Reponses were invited from directors of training programs who were recruited via email to leaders of national organizations. The survey was completed by 80 directors from 24 countries who directed 113 programs. The largest numbers of respondents directed postdoctoral training of scientists (42%) or physicians (33%), post-masters degree programs (33%), and PhD programs (29%). Most programs (82%) were 2years or longer in duration. Formal training was offered in research ethics by 39%, medical ethics by 31%, professional ethics by 24% and business ethics by 9%. The number of reported hours of formal training varied widely, e.g., from 0 to >15h/year for research ethics and from 0 to >15h for medical ethics. Ethics training was required and/or tested in 75% of programs that offered training. A majority (54%) of respondents reported plans to add or enhance training in ethics; many indicated a desire for online resources related to ethics, especially resources with self-assessment tools. Formal teaching of ethics is absent from many training programs in clinical chemistry and laboratory medicine, with heterogeneity in the extent and methods of ethics training among the programs that provide the training. A perceived need exists for online training tools, especially tools with self-assessment components. Copyright © 2014 Elsevier B.V. All rights reserved.

  18. Guinea pig maximization tests with formaldehyde releasers. Results from two laboratories

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Boman, A; Hamann, K

    1984-01-01

    The guinea pig maximization test was used to evaluate the sensitizing potential of formaldehyde and 6 formaldehyde releasers (Forcide 78, Germall 115, Grotan BK, Grotan OX, KM 200 and Preventol D2). The tests were carried out in 2 laboratories (Copenhagen and Stockholm), and although we intended...... the procedures to be the same, discrepancies were observed, possibly due to the use of different animal strains, test concentrations and vehicles. The sensitizing potential was in general found to be stronger in Stockholm compared to Copenhagen: formaldehyde sensitized 50% of the guinea pigs in Copenhagen and 95...

  19. Model-based reasoning in the physics laboratory: Framework and initial results

    Science.gov (United States)

    Zwickl, Benjamin M.; Hu, Dehui; Finkelstein, Noah; Lewandowski, H. J.

    2015-12-01

    [This paper is part of the Focused Collection on Upper Division Physics Courses.] We review and extend existing frameworks on modeling to develop a new framework that describes model-based reasoning in introductory and upper-division physics laboratories. Constructing and using models are core scientific practices that have gained significant attention within K-12 and higher education. Although modeling is a broadly applicable process, within physics education, it has been preferentially applied to the iterative development of broadly applicable principles (e.g., Newton's laws of motion in introductory mechanics). A significant feature of the new framework is that measurement tools (in addition to the physical system being studied) are subjected to the process of modeling. Think-aloud interviews were used to refine the framework and demonstrate its utility by documenting examples of model-based reasoning in the laboratory. When applied to the think-aloud interviews, the framework captures and differentiates students' model-based reasoning and helps identify areas of future research. The interviews showed how students productively applied similar facets of modeling to the physical system and measurement tools: construction, prediction, interpretation of data, identification of model limitations, and revision. Finally, we document students' challenges in explicitly articulating assumptions when constructing models of experimental systems and further challenges in model construction due to students' insufficient prior conceptual understanding. A modeling perspective reframes many of the seemingly arbitrary technical details of measurement tools and apparatus as an opportunity for authentic and engaging scientific sense making.

  20. Cold Climate Foundation Retrofit Experimental Hygrothermal Performance: Cloquet Residential Research Facility Laboratory Results

    Energy Technology Data Exchange (ETDEWEB)

    Goldberg, Louise F. [NorthernSTAR, St. Paul, MN (United States); Harmon, Anna C. [NorthernSTAR, St. Paul, MN (United States)

    2015-04-01

    Thermal and moisture problems in existing basements create a unique challenge because the exterior face of the wall is not easily or inexpensively accessible. This approach addresses thermal and moisture management from the interior face of the wall without disturbing the exterior soil and landscaping. the interior and exterior environments. This approach has the potential for improving durability, comfort, and indoor air quality. This project was funded jointly by the National Renewable Energy Laboratory (NREL) and Oak Ridge National Laboratory (ORNL). ORNL focused on developing a full basement wall system experimental database to enable others to validate hygrothermal simulation codes. NREL focused on testing the moisture durability of practical basement wall interior insulation retrofit solutions for cold climates. The project has produced a physically credible and reliable long-term hygrothermal performance database for retrofit foundation wall insulation systems in zone 6 and 7 climates that are fully compliant with the performance criteria in the 2009 Minnesota Energy Code. The experimental data were configured into a standard format that can be published online and that is compatible with standard commercially available spreadsheet and database software.

  1. Chlorophyll fluorescence of epiphytic lichens under acid, ammonium and fungicide stress - a comparison of laboratory and field results

    Energy Technology Data Exchange (ETDEWEB)

    Luebke, K.; Tremp, H. [Hohenheim Univ., Stuttgart (Germany). Inst. for Landscape and Plant Ecology

    2002-07-01

    Fungicide treatment (dithiocarbamate) caused a significant reduction of the quantum yield of photochemical energy conversion of the tested lichen species Parmelia sulcata and Parmelia subrudecta under laboratory conditions. Especially P. subrudecta showed a rapid decrease of photosynthetic capacity in between one week and was more susceptible to fungicide stress compared to Parmelia sulcata. Over the time span of four weeks, sulphuric acid and ammonium sulphate induced no significant changes of fluorescence parameters. At natural stands (lichens on apple tree bark) the same fungicide was applied. Here no vitality-decline (visual verification, yield parameter) was detectable. So results from laboratory are not transferable on lichen living under natural habitat conditions, because removing lichens from tree bark causes additional stress to species. Results obtained from ecotoxicological laboratory experiments should be carefully proven and if possible completed and verified under more realistic background situations. (orig.)

  2. Inter-laboratory consistency and variability in the buccal micronucleus cytome assay depends on biomarker scored and laboratory experience: results from the HUMNxl international inter-laboratory scoring exercise.

    Science.gov (United States)

    Bolognesi, Claudia; Knasmueller, Siegfried; Nersesyan, Armen; Roggieri, Paola; Ceppi, Marcello; Bruzzone, Marco; Blaszczyk, Ewa; Mielzynska-Svach, Danuta; Milic, Mirta; Bonassi, Stefano; Benedetti, Danieli; Da Silva, Juliana; Toledo, Raphael; Salvadori, Daisy Maria Fávero; Groot de Restrepo, Helena; Filipic, Metka; Hercog, Klara; Aktas, Ayça; Burgaz, Sema; Kundi, Michael; Grummt, Tamara; Thomas, Philip; Hor, Maryam; Escudero-Fung, Maria; Holland, Nina; Fenech, Michael

    2017-03-01

    The buccal micronucleus cytome (BMNcyt) assay in uncultured exfoliated epithelial cells from oral mucosa is widely applied in biomonitoring human exposures to genotoxic agents and is also proposed as a suitable test for prescreening and follow-up of precancerous oral lesions. The main limitation of the assay is the large variability observed in the baseline values of micronuclei (MNi) and other nuclear anomalies mainly related to different scoring criteria. The aim of this international collaborative study, involving laboratories with different level of experience, was to evaluate the inter- and intra-laboratory variations in the BMNcyt parameters, using recently implemented guidelines, in scoring cells from the same pooled samples obtained from healthy subjects (control group) and from cancer patients undergoing radiotherapy (treated group). The results indicate that all laboratories correctly discriminated samples from the two groups by a significant increase of micronucleus (MN) and nuclear bud (NBUD) frequencies and differentiated binucleated (BN) cells, associated with the exposure to ionizing radiation. The experience of the laboratories was shown to play an important role in the identification of the different cell types and nuclear anomalies. MN frequency in differentiated mononucleated (MONO) and BN cells showed the greatest consistency among the laboratories and low variability was also detected in the frequencies of MONO and BN cells. A larger variability was observed in classifying the different cell types, indicating the subjectivity in the interpretation of some of the scoring criteria while reproducibility of the results between scoring sessions was very good. An inter-laboratory calibration exercise is strongly recommended before starting studies with BMNcyt assay involving multiple research centers. © The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions

  3. Test Results From The Idaho National Laboratory 15kW High Temperature Electrolysis Test Facility

    Energy Technology Data Exchange (ETDEWEB)

    Carl M. Stoots; Keith G. Condie; James E. O' Brien; J. Stephen Herring; Joseph J. Hartvigsen

    2009-07-01

    A 15kW high temperature electrolysis test facility has been developed at the Idaho National Laboratory under the United States Department of Energy Nuclear Hydrogen Initiative. This facility is intended to study the technology readiness of using high temperature solid oxide cells for large scale nuclear powered hydrogen production. It is designed to address larger-scale issues such as thermal management (feed-stock heating, high temperature gas handling, heat recuperation), multiple-stack hot zone design, multiple-stack electrical configurations, etc. Heat recuperation and hydrogen recycle are incorporated into the design. The facility was operated for 1080 hours and successfully demonstrated the largest scale high temperature solid-oxide-based production of hydrogen to date.

  4. X-ray diffraction results from Mars Science Laboratory: mineralogy of Rocknest at Gale crater.

    Science.gov (United States)

    Bish, D L; Blake, D F; Vaniman, D T; Chipera, S J; Morris, R V; Ming, D W; Treiman, A H; Sarrazin, P; Morrison, S M; Downs, R T; Achilles, C N; Yen, A S; Bristow, T F; Crisp, J A; Morookian, J M; Farmer, J D; Rampe, E B; Stolper, E M; Spanovich, N

    2013-09-27

    The Mars Science Laboratory rover Curiosity scooped samples of soil from the Rocknest aeolian bedform in Gale crater. Analysis of the soil with the Chemistry and Mineralogy (CheMin) x-ray diffraction (XRD) instrument revealed plagioclase (~An57), forsteritic olivine (~Fo62), augite, and pigeonite, with minor K-feldspar, magnetite, quartz, anhydrite, hematite, and ilmenite. The minor phases are present at, or near, detection limits. The soil also contains 27 ± 14 weight percent x-ray amorphous material, likely containing multiple Fe(3+)- and volatile-bearing phases, including possibly a substance resembling hisingerite. The crystalline component is similar to the normative mineralogy of certain basaltic rocks from Gusev crater on Mars and of martian basaltic meteorites. The amorphous component is similar to that found on Earth in places such as soils on the Mauna Kea volcano, Hawaii.

  5. Early Results on Radioactive Background Characterization for Sanford Laboratory and DUSEL Experiments

    CERN Document Server

    Mei, D -M; Thomas, K; Gray, F

    2009-01-01

    Measuring external sources of background for a deep underground laboratory at the Homestake Mine is an important step for the planned low-background experiments. The naturally occurring $\\gamma$-ray fluxes at different levels in the Homestake Mine are studied using NaI detectors and Monte Carlo simulations. A simple algorithm is developed to convert the measured $\\gamma$-ray rates into $\\gamma$-ray fluxes. A good agreement between the measured and simulated $\\gamma$-ray fluxes is achieved with the knowledge of the chemical composition and radioactivity levels in the rock. The neutron fluxes and $\\gamma$-ray fluxes are predicted by Monte Carlo simulations for different levels including inaccessible levels that are under construction for the planned low background experiments.

  6. Comparison of a laboratory spectrum of Eu-152 with results of simulation using the MCNP code

    Energy Technology Data Exchange (ETDEWEB)

    Rodenas, J. [Departamento de Ingenieria Quimica y Nuclear, Universidad Politecnica de Valencia, Apartado 22012, E-46071 Valencia (Spain); Gallardo, S. [Departamento de Ingenieria Quimica y Nuclear, Universidad Politecnica de Valencia, Apartado 22012, E-46071 Valencia (Spain)], E-mail: sergalbe@iqn.upv.es; Ortiz, J. [Laboratorio de Radiactividad Ambiental, Universidad Politecnica de Valencia, Apartado 22012, E-46071 Valencia (Spain)

    2007-09-21

    Detectors used for gamma spectrometry must be calibrated for each geometry considered in environmental radioactivity laboratories. This calibration is performed using a standard solution containing gamma emitter sources. Nevertheless, the efficiency curves obtained are periodically checked using a source such as {sup 152}Eu emitting many gamma rays that cover a wide energy range (20-1500 keV). {sup 152}Eu presents a problem because it has a lot of peaks affected by True Coincidence Summing (TCS). Two experimental measures have been performed placing the source (a Marinelli beaker) at 0 and 10 cm from the detector. Both spectra are simulated by the MCNP 4C code, where the TCS is not reproduced. Therefore, the comparison between experimental and simulated peak net areas permits one to choose the most convenient peaks to check the efficiency curves of the detector.

  7. Direction-sensitive dark matter search results in a surface laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Miuchi, Kentaro [Cosmic-Ray Group, Department of Physics, Graduate School of Science, Kyoto University Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)], E-mail: miuchi@cr.scphys.kyoto-u.ac.jp; Hattori, Kaori; Kabuki, Shigeto; Kubo, Hidetoshi; Kurosawa, Shunsuke; Nishimura, Hironobu; Okada, Yoko; Takada, Atsushi; Tanimori, Toru; Tsuchiya, Ken' ichi; Ueno, Kazuki [Cosmic-Ray Group, Department of Physics, Graduate School of Science, Kyoto University Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan); Sekiya, Hiroyuki; Takeda, Atsushi [Kamioka Observatory, ICRR, University of Tokyo Higashi-Mozumi, Kamioka cho, Hida 506-1205 (Japan)

    2007-10-11

    We developed a three-dimensional gaseous tracking device and performed a direction-sensitive dark matter search in a surface laboratory. By using 150 Torr carbon-tetrafluoride (CF{sub 4}) gas, we obtained a sky map drawn with the recoil directions of the carbon and fluorine nuclei, and set the first limit on the spin-dependent WIMP (Weakly Interacting Massive Particles)-proton cross section by a direction-sensitive method. Thus, we showed that a WIMP-search experiment with a gaseous tracking device can actually set limits. Furthermore, we demonstrated that this method will potentially play a certain role in revealing the nature of dark matter when a low-background large-volume detector is developed.

  8. Model-Based Reasoning in the Upper-Division Physics Laboratory: Framework and Initial Results

    CERN Document Server

    Zwickl, Benjamin M; Finkelstein, Noah; Lewandowski, H J

    2014-01-01

    Constructing and using models are core scientific practices that have gained significant attention within K-12 and higher education. Although modeling is a broadly applicable process, within physics education, it has been preferentially applied to the iterative development of broadly applicable principles (e.g., Newton's laws of motion in introductory mechanics). We review and extend existing frameworks on modeling to develop a new framework that more naturally describes model-based reasoning in upper-division physics labs. A significant feature of the new framework is that measurement tools (in addition to the physical system being studied) are subjected to the process of modeling. Think-aloud interviews were used to document examples of model-based reasoning in the laboratory and refine the modeling framework. The interviews showed how students productively applied similar facets of modeling to the physical system and measurement tools: construction, prediction, interpretation of data, identification of mod...

  9. Evaluation of selected martensitic stainless steels for use in downhole tubular expansion - Results of a laboratory study

    Energy Technology Data Exchange (ETDEWEB)

    Mack, Robert [Shell International E and P, b.v. Kessler Park 1, Postbus 60, 2280 AB Rijswijk (Netherlands)

    2004-07-01

    A laboratory program was performed to evaluate the potential of selected martensitic stainless steels for downhole cladding applications. The evaluation of the effects of tubular expansion on mechanical properties, defects, and resistance to environmentally assisted cracking demonstrated that some steels were acceptable for the intended application. The results were used to qualify and select the stainless steel for the intended sweet cladding applications. (authors)

  10. Laboratory and field testing results of the LMT/GTM primary surface actuators

    Science.gov (United States)

    Smith, David R.; Souccar, Kamal; Montalvo, Gabriela; Arteaga Magaña, César; Hernández Rebollar, José Luis; Olmos Tapia, Arak; Gallieni, Daniele; Lazzarini, Paolo; Fumi, Pierluigi; Anaclerio, Enzo

    2016-07-01

    With the final installation of the two outermost rings of the primary surface of the Large Millimeter Telescope/ Gran Telescopio Milimétrico (LMT/GTM), the project is also upgrading the primary surface actuators. There are commercial actuators that can approach the required operational accuracy and stroke, but the combination of the size and load requirements ultimately required a customized design. The new actuators fit within the volume constraints imposed by the tighter interior angles in the outer rings and are designed to support the operational and survival loading conditions even for the largest surface segments. Laboratory testing confirmed that the actuators should meet the precision, repeatability, load, and lifetime requirements. However, the LMT/GTM is at a particularly difficult site for electromechanical systems. The high altitude has the usual effect of reducing cooling effectiveness for the drives and motors, and the ambient temperature hovers near freezing. Since there is a significant amount of precipitation during some times of the year, there are frequent freeze/thaw cycles. The constant formation and either sublimation or melting of ice, along with the associated high humidity, has been a challenge for the environmental protection of many devices at the LMT/GTM. Because there are a total of 720 primary surface actuators in the system, it is particularly important that the actuators, their local drive control boxes, and their cable connections be able to meet its specifications even under the site conditions. To confirm the suitability of the actuators, the LMT/GTM procured an initial set of sixteen actuators for testing at the site. After laboratory testing, the actuators were installed into the outer two rings of the telescope and cycled during the early winter months of the 2015-16 scientific observing season. Because of the continuing installation activities in these two rings, they are not illuminated by the receivers, so field testing

  11. Carcinogenicity of residual fuel oils by nonbiological laboratory methods: annotated bibliography. Part I. Laboratory methods of analysis. Part II. Analysis results

    Energy Technology Data Exchange (ETDEWEB)

    Cichorz, R. S.

    1976-04-09

    Recent emphases have been directed by Federal government regulatory agencies and other research groups on the carcinogenic effects of certain aromatic hydrocarbon components in naturally occurring petroleum products. These are used in plant operations, and underline the importance of evaluating environments. Since Rocky Flats Plant uses large quantities of fuel oil, the author was prompted to undertake a search of the chemical literature. Articles and accounts of studies were reviewed on nonbiological laboratory methods for determining the carcinogenicity of residual fuel oils and related high-boiling petroleum fractions. The physical and chemical methods involve the separation or measurement (or both) of polynuclear aromatic constituents which generally are responsible for the carcinogenic effects. Thus, the author suggests that the total carcinogenic activity of any petroleum product may not be due to a specific potent carcinogen, but rather to the cumulative effect of several individually weak carcinogens. The literature search is presented as an annotated bibliography, current as of January 1, 1975, and includes significant parts of the studies along with the total number of other references found when the citation was examined in its entirety. Part I deals with laboratory chemical and physical methods of determining carcinogenicity or polynuclear aromatic hydrocarbons (or both) in residual fuel oils and contains ten entries. Part II includes the results of testing specific fuel oils for carcinogenic constituents and contains eleven entries. An author index and subject categories are included.

  12. Laboratory spectroscopy of Mars Analogue materials and latest field results from Iceland and Eifel

    Science.gov (United States)

    Offringa, Marloes; Foing, Bernard H.

    2016-07-01

    We have established a collection of samples, and measured them in the laboratory towards a spectrometric database that could be used as a reference for future orbital or in situ measurements. We are using systematically for all samples UV-VIS and NIR reflectance spectrometers, and sporadically a Fourier Transform Infrared (FTIR) spectrometer, an X-Ray Fluorescence (XRF) spectrometer and a Raman laser spectrometer on control samples. We also used a documented set of Moon-Mars relevant minerals curated at VU Amsterdam, as well as samples retrieved from Mars analogue campaigns in Utah (Foing et al., 2011, 2016), Iceland (Mid-Atlantic ridge spreading and magma-ice interaction), La Réunion hot spot volcano and Eifel volcanic region (mixed hotspot and melt-ascent through crust fractures) from recent campaigns in 2015 and 2016.. We discuss samples spectral diagnostics of volcanic processes and hydrous alterations that can inform recent or upcoming measurements from Mars orbit or in situ rovers. Acknowledgements: we thank Dominic Doyle for ESTEC optical lab support, Euan Monaghan (Leiden U) for FTIR measurement support, Wim van Westrenen for access to VU samples, Oscar Kamps (Utrecht U), Aidan Cowley (EAC) and Matthias Sperl (DLR) for support discussions

  13. Preliminary results of orthotopic en bloc uterus and ovary transplantation in the laboratory rat.

    Science.gov (United States)

    Motoc, A; Jiga, L; Ionac, M; Raica, M; Motoc, M; Chiovschi, S

    2003-01-01

    A new experimental model of whole uterus and ovary transplantation in the laboratory rat was achieved. The main goals of this study were concerned with developing and standardizing the microsurgical technique of uterus transplantation in rats and observing the particular cellular patterns of acute allograft rejection at the level of the transplanted graft. Thirty-five orthotopic uterus transplantations were performed. An additional 20 female rats were used for dissection training sessions. Recipients were euthanasied at 24 hours, 48 hours and 72 hours. Immediate postoperative survival was 100%. Patency of the microsurgical anastomoses, checked at 24 hours, was 100%. At 72 hours thrombosis occurred in all anastomoses. The explanted uterine grafts were fixed in formaline and analyzed under light microscopy and specific imunohistochemical analysis. The acute allograft rejection has a particular cellular reaction pattern, probably due to the unique diversity of the tissues that compose it. Inflammatory cells like LTCD8+, LBCD20+ and mastocytes tend to agglomerate in the vicinity of nervous and vascular structures, showing no signs of lymphoid tissue disposition like in typical acute rejection. Uterus transplantation in rats has proven to be a valid experiment that allows us to express hope that by further research on transplantation of the uterus gynecologists will be able to introduce an adapted technique in the treatment of specific cases of human female infertility.

  14. Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014.

    Science.gov (United States)

    Van der Auwera, Gert; Bart, Aldert; Chicharro, Carmen; Cortes, Sofia; Davidsson, Leigh; Di Muccio, Trentina; Dujardin, Jean-Claude; Felger, Ingrid; Paglia, Maria Grazia; Grimm, Felix; Harms, Gundel; Jaffe, Charles L; Manser, Monika; Ravel, Christophe; Robert-Gangneux, Florence; Roelfsema, Jeroen; Töz, Seray; Verweij, Jaco J; Chiodini, Peter L

    2016-12-08

    Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were reported. The overall error rate of strains placed in the wrong complex or species was 8.5%. Various reasons for incorrect typing were identified. The study shows there is considerable room for improvement and standardisation of Leishmania typing. The use of well validated standard operating procedures is recommended, covering testing, interpretation, and reporting guidelines. Application of the internal transcribed spacer 1 of the rDNA array should be restricted to Old World samples, while the heat-shock protein 70 gene and the mini-exon can be applied globally. This article is copyright of The Authors, 2016.

  15. Results of external review Sandia National Laboratories microelectronics and photonics program (October 2002).

    Energy Technology Data Exchange (ETDEWEB)

    Peercy, Paul S. (University of Wisconsin, Madison, WI); Myers, David R.

    2003-10-01

    The US Department of Energy requires a periodic 'self assessment' of Sandia's Microsystems Program. An external panel review of this program is held approximately every 18 months, and the report from the external review panel serves as the basis for the DOE 'self assessment.' The review for this fiscal year was held on September 30-October 1, 2002 at Sandia National Laboratories, Albuquerque, NM. The panel was comprised of experts in the fields of microelectronics, photonics and microsystems from universities, industry and other Government agencies. A complete list of the panel members is shown as Appendix A to the attached report. The review assesses four areas: relevance to national needs and agency mission; quality of science technology and engineering; performance in the operation of a major facility; and program performance management and planning. Relevance to national needs and agency mission was rated as 'outstanding.' The quality of science, technology, and engineering was rated as 'outstanding.' Operation of a major facility was noted as 'outstanding,' while the category of program performance, management, and planning was rated as 'outstanding.' Sandia's Microsystems Program received an overall rating of 'outstanding' [the highest possible rating]. The attached report was prepared by the panel in a format requested by Sandia to conform with the performance criteria for the DOE self assessment.

  16. Initial results from a laboratory emulation of weak gravitational lensing measurements

    CERN Document Server

    Seshadri, Suresh; Goodsall, Timothy; Fucik, Jason; Hirata, Christopher M; Rhodes, Jason; Rowe, Barnaby; Smith, Roger

    2013-01-01

    Weak gravitational lensing observations are a key science driver for the NASA Wide Field Infrared Survey Telescope (WFIRST). To validate the performance of the WFIRST infrared detectors, we have performed a laboratory emulation of weak gravitational lensing measurements. Our experiments used a custom precision projector system to image a target mask composed of a grid of pinholes, emulating stellar point sources, onto a 1.7 micron cut-off Teledyne HgCdTe/H2RG detector. We used a 880nm LED illumination source and f/22 pupil stop to produce undersampled point spread functions similar to those expected from WFIRST. We also emulated the WFIRST image reconstruction strategy, using the IMage COMbination (IMCOM) algorithm to derive oversampled images from dithered, undersampled input images. We created shear maps for this data and computed shear correlation functions to mimic a real weak lensing analysis. After removing only 2nd order polynomial fits to the shear maps, we found that the correlation functions could b...

  17. Patient navigation and time to diagnostic resolution: results for a cluster randomized trial evaluating the efficacy of patient navigation among patients with breast cancer screening abnormalities, Tampa, FL.

    Directory of Open Access Journals (Sweden)

    Ji-Hyun Lee

    Full Text Available The objective of this study was to evaluate a patient navigation (PN program that attempts to reduce the time between a breast cancer screening abnormality and definitive diagnosis among medically underserved populations of Tampa Bay, Florida.The Moffitt Patient Navigation Research Program conducted a cluster randomized design with 10 primary care clinics. Patients were navigated from time of a breast screening abnormality to diagnostic resolution. This paper examined the length of time between breast abnormality and definitive diagnosis, using a shared frailty Cox proportional hazard model to assess PN program effect.1,039 patients were eligible for the study because of an abnormal breast cancer screening/clinical abnormality (494 navigated; 545 control. Analysis of PN effect by two time periods of resolution (0-3 months and > 3 months showed a lagged effect of PN. For patients resolving in the first three months, the adjusted Hazard Ratio (aHR was 0.85 (95% Confidence Interval [CI]: 0.64-1.13 suggesting that PN had no effect on resolution time during this period. Beyond three months, however, navigated patients resolved more quickly to diagnostic resolution compared with the control group (aHR 2.8, 95%CI: 1.30-6.13. The predicted aHR at 3 months was 1.2, which was not statistically significant, while PN had a significant positive effect beyond 4.7 months.PN programs may increase the timeliness of diagnostic resolution for patients with a breast cancer-related abnormality. PN did not speed diagnostic resolution during the initial three months of follow up but started to reduce time to diagnostic resolution after three months and showed a significant effect after 4.7 months.ClinicalTrials.gov NCT00375024.

  18. Groundwater monitoring at three Oak Ridge National Laboratory inactive waste impoundments: results after one year

    Energy Technology Data Exchange (ETDEWEB)

    Francis, C. W.; Stansfield, R. G.

    1986-10-01

    To determine if the migration of potential contaminants from three inactive waste impoundments at Oak Ridge National Laboratory poses a threat to groundwater quality, at least one upgradient groundwater monitoring well and threee downgradient monitoring wells were installed at each impoundment in early 1985. These three unlined impoundments, formerly used to collect and, in some instances, treat wastewater are: the 3513 impoundment; the Old Hydrofracture Facility (OHF) impoundment; and the Homogeneous Reactor Experimnt No. 2 impoundment. Groundwater samples were collected quarterly for one year. Analyses were conducted for the groundwater protection parameters promulgated by the Resource Conservation and Recovery Act. The groundwater samples were also analyzed for polychlorinated biphenyls, copper, nickel, zinc, /sup 90/Sr, /sup 137/Cs, and tritium. The contaminants found most often to affect groundwater quality at all three waste impoundments were radionuclides. For example, mean concentrations of gross beta and gross alpha activity exceeded drinking water limits at all three sites. The gross beta limit was exceeded at the 3513 and OHF impoundments by either /sup 90/Sr or tritium levels. At the 3513 impoundment, there was substantial evidence that the downgradient groundwater has been contaminated by chromium and lead and possibly by halogenated organic compounds. At the OHF impoundment, the mean level of tritium measured in the upgradient well (about 91,000 Bq/L as compared with 80,000 Bq/L in the downgradient wells) indicated that the groundwater quality has been affected by the radioactive wastes buried in the low-level radioactive waste burial ground solid waste storage area-5 upgradient of the impoundment. Testing for groundwater contamination, disclosed statistically significant contamination at all three sites.

  19. Results of the Dyslipidemia International Study (DYSIS-Middle East: clinical perspective on the prevalence and characteristics of lipid abnormalities in the setting of chronic statin treatment.

    Directory of Open Access Journals (Sweden)

    Saud N Al Sifri

    Full Text Available BACKGROUND: Therapeutic intervention with low-density lipoprotein cholesterol-lowering agents known as statins has been demonstrated to reduce cardiovascular risk. However, many patients on statin treatment have persistent dyslipidemia and remain at a high risk of cardiovascular disease. Therefore, the objective of this study was to assess the frequency of lipid abnormalities in patients receiving chronic statin treatment. METHODS: As part of an international, cross-sectional, observational study, DYSIS-Middle East enrolled 2,182 patients in the United Arab Emirates (UAE, Saudi Arabia, Lebanon and Jordan. All patients were over 45 years of age and had been on statin treatment for at least three months. Data on demographics, lipid parameters and cardiovascular risk profile were recorded. Cardiovascular risk was defined according the guidelines of the European Society of Cardiology. RESULTS: The majority of patients (82.6% were classified as being at very high risk of cardiovascular events, and 61.8% of all patients did not attain LDL-C target levels. Low high-density lipoprotein cholesterol levels and elevated triglyceride levels were noted in 55.5% and 48.5% of patients, respectively. Multivariate logistical regression modeling indicated that factors independently associated with LDL-C levels not being at goal were lifestyle choices, diabetes mellitus, ischemic heart disease, and blood pressure ≥ 140/90 mmHg. CONCLUSIONS: Almost two-thirds of statin-treated patients in the United Arab Emirates, Saudi Arabia, Lebanon and Jordan had inadequately controlled lipid levels. More comprehensive surveillance, awareness and treatment regimens, as well as modification of lifestyle choices, is necessary to halt the rise in cardiovascular disease-related mortality.

  20. Morphological abnormalities in Japanese red pine (Pinus densiflora) at the territories contaminated as a result of the accident at Fukushima Dai-Ichi Nuclear Power Plant.

    Science.gov (United States)

    Yoschenko, Vasyl; Nanba, Kenji; Yoshida, Satoshi; Watanabe, Yoshito; Takase, Tsugiko; Sato, Natsumi; Keitoku, Koji

    2016-12-01

    Our research, carried out in 2014-2016 at eight sites in the radioactive contaminated territories of Fukushima Prefecture, showed that the young trees of Japanese red pine (Pinus densiflora) are sensitive to radiation. Irradiation induced cancellation of the apical dominance in this species. The effect is similar to that observed in young trees of Scots pine growing in the Chernobyl zone. At the same time, we did not observed any morphological abnormalities in mature trees of Japanese red pine. The probability of cancelling the apical dominance in Japanese red pine increased to 0.11 and 0.14 in the two less irradiated populations, and to 0.5 and 0.9 at sites were the absorbed dose rates were approximately 14 and 25 μGy h(-1), respectively. Most of the observed abnormalities appeared in the second whorl after the beginning of exposure. No new abnormalities were observed in the fifth whorl. This temporal pattern is similar to those reported for Scots pine in Chernobyl and for Japanese fir in Fukushima. Additional detailed studies are necessary for interpretation of the observed temporal pattern and, in general, for explanation of the mechanism of formation of the morphological abnormalities.

  1. Deletion of Hemojuvelin, an Iron-Regulatory Protein, in Mice Results in Abnormal Angiogenesis and Vasculogenesis in Retina Along With Reactive Gliosis

    OpenAIRE

    2014-01-01

    Deletion of Hemojuvelin (HJV) in mice is associated with excessive iron accumulation and morphologic changes in the retina. In this study, we find that HJV knockout mice have abnormal retinal angiogenesis and vasculogenesis along with robust reactive gliosis involving Müller cells and microglia.

  2. The clinical features, laboratory findings, treatment and follow-up results of patients with morphea

    Directory of Open Access Journals (Sweden)

    Nehir Parlak

    2013-12-01

    Full Text Available Objective: Morphea, also known as localized scleroderma, is a rare skin disease of unknown pathogenesis, characterized by fibrosis in the skin and subcutaneous tissue. In this study, we aim to evaluate the demographic features, clinical characteristics, laboratory findings, and response to treatment in patients diagnosed with morphea. Materials and Methods: The findings of fifty eight patients diagnosed with morphea were retrospectively evaluated between 1995-2011. All patients' clinical symptoms, concomitant diseases, symptoms, immunological features and presence of peripheral eosinophilia were investigated. Treatment methods, response to therapy of 40 patients whose treatment continued for 2-12 months were examined. Fourty nine patients (84.5% were female and 9 patients (15.5% were male of 58 patients who were diagnosed with morphea. The mean age of patients was 42.33±18.44 years (range: 7-75 years. Diagnosis was made histopathologically in all cases. Borrelia antibodies were negative in all patients enrolling the study. Thirty six patients (62.1% had plaque type, 17 patients (29.3% had generalized type, 3 patients (5.2% had mixed type (linear + plaque and 2 patients (3.4% had linear type of morphea. ANA was found to be positive in 12 (26.2% of 46 patients. Considering the relationship between the clinical types of morphea with ANA, 38.5% of plaque type, 53.8% of generalized type, 7.7% of mixed type patients showed ANA positivity. ANA positivity was statistically significant in patients with generalized morphea (p=0.027. Peripheral eosinophilia was detected in one case in whom lesions were generalized (2.1%. Colchicine therapy was given to 23 cases. Complete and partial response rates are 47.8% and 26.1%, respectively. However, 17.4% of patients remained stable and progression was noted in 8.7% of the cases. Conclusion: In conclusion, plaque type morphea is the most common type of morphea. ANA positivity was statistically significant in

  3. Public health consequences of a false-positive laboratory test result for Brucella--Florida, Georgia, and Michigan, 2005.

    Science.gov (United States)

    2008-06-01

    Human brucellosis, a nationally notifiable disease, is uncommon in the United States. Most human cases have occurred in returned travelers or immigrants from regions where brucellosis is endemic, or were acquired domestically from eating illegally imported, unpasteurized fresh cheeses. In January 2005, a woman aged 35 years who lived in Nassau County, Florida, received a diagnosis of brucellosis, based on results of a Brucella immunoglobulin M (IgM) enzyme immunoassay (EIA) performed in a commercial laboratory using analyte specific reagents (ASRs); this diagnosis prompted an investigation of dairy products in two other states. Subsequent confirmatory antibody testing by Brucella microagglutination test (BMAT) performed at CDC on the patient's serum was negative. The case did not meet the CDC/Council of State and Territorial Epidemiologists' (CSTE) definition for a probable or confirmed brucellosis case, and the initial EIA result was determined to be a false positive. This report summarizes the case history, laboratory findings, and public health investigations. CDC recommends that Brucella serology testing only be performed using tests cleared or approved by the Food and Drug Administration (FDA) or validated under the Clinical Laboratory Improvement Amendments (CLIA) and shown to reliably detect the presence of Brucella infection. Results from these tests should be considered supportive evidence for recent infection only and interpreted in the context of a clinically compatible illness and exposure history. EIA is not considered a confirmatory Brucella antibody test; positive screening test results should be confirmed by Brucella-specific agglutination (i.e., BMAT or standard tube agglutination test) methods.

  4. Chromosome Abnormalities

    Science.gov (United States)

    ... are two kinds of cell division, mitosis and meiosis. Mitosis results in two cells that are duplicates ... make up our body are made and replaced. Meiosis results in cells with half the number of ...

  5. Prioritization methodology using hazard analysis results at Los Alamos National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Sasser, M.K.; Hall, M.; Stack, D. [Los Alamos National Lab., NM (United States); Brooks, D.G. [Arizona State Univ. (United States)

    1995-09-01

    Risk management activities, such as prioritizing risk-reducing projects, often are commissioned for facilities as special tasks supported by special task forces and conducted independently of other on-going risk assessment and risk management activities. Many DOE facilities have completed hazard analyses (HAs) as part of their efforts to upgrade their SARs to meet the new DOE standard that was issued in 1994. Although a complete SAR would contain more resource allocation information than the HA, the HA usually is completed before the SAR. This paper describes how SAR results, and particularly HA results, can be used directly to support managers` risk-based prioritization of project funding. This can reduce the time to conduct prioritization modeling, increase the quality of the results, and, perhaps most importantly, integrate the results into the on-going risk management activities of the site.

  6. Compendium of Recent Test Results of Single Event Effects Conducted by the Jet Propulsion Laboratory

    Science.gov (United States)

    Allen, Gregory R.; Guertin, Steven M.; Scheick, Leif Z.; Irom, Farokh; Zajac, Stephanie

    2012-01-01

    This paper reports heavy ion, proton, and laser induced single event effects results for a variety of microelectronic devices targeted for possible use in NASA spacecrafts. The compendium covers devices tested within the years of 2010 through 2012.

  7. Flue gas conditioning for improved particle collection in electrostatic precipitators. First topical report, Results of laboratory screening of additives

    Energy Technology Data Exchange (ETDEWEB)

    Durham, M.D.

    1993-04-16

    Several tasks have been completed in a program to evaluate additives to improve fine particle collection in electrostatic precipitators. Screening tests and laboratory evaluations of additives are summarized in this report. Over 20 additives were evaluated; four were found to improve flyash precipitation rates. The Insitec particle analyzer was also evaluated; test results show that the analyzer will provide accurate sizing and counting information for particles in the size range of {le} 10 {mu}m dia.

  8. BAY 81-8973, a full-length recombinant factor VIII: results from an International comparative laboratory field study.

    Science.gov (United States)

    Kitchen, S; Beckmann, H; Katterle, Y; Bruns, S; Tseneklidou-Stoeter, D; Maas Enriquez, M

    2016-05-01

    BAY 81-8973 is a full-length, unmodified, recombinant human factor VIII (FVIII) with the same primary amino acid sequence as sucrose-formulated recombinant FVIII but produced with certain more advanced manufacturing technologies. This global laboratory study evaluated variability in measurement of BAY 81-8973 using one-stage and chromogenic assays compared with antihaemophilic factor (recombinant) plasma/albumin-free method (rAHF-PFM; Advate(®) ) under assay conditions routinely used in clinical laboratories. BAY 81-8973 or rAHF-PFM was spiked into FVIII-deficient plasma at 0.043 (low), 0.375 (medium) and 0.865 (normal) IU mL(-1) . Participating laboratories analysed blinded samples and normal plasma in triplicate using their routine assay, reagents and standards. Results were analysed for intra- and interlaboratory variability. Forty-one laboratories in 11 countries participated in the study. One-stage assay and chromogenic assays were used by 40 and 10 laboratories, respectively; 9 laboratories used both assays. Intralaboratory variability was <11% for both assays and both products at all concentrations. Interlaboratory variability was highest at the low concentration in the chromogenic and one-stage assay for BAY 81-8973 (60.0% and 33.7%, respectively) and rAHF-PFM (51.0% and 30.8%) and was lowest at the normal concentration (BAY 81-8973, 5.4% and 14.0%; rAHF-PFM, 5.8% and 12.4%), which was similar to the plasma control (6.6% and 10.3%). The chromogenic:one-stage assay ratio ranged from 0.95 (low concentration) to 1.10 (normal concentration) for BAY 81-8973 and 0.96-1.18 for rAHF-PFM. BAY 81-8973 can be accurately measured in plasma using the one-stage and chromogenic assays routinely used in clinical laboratories without a product-specific standard. © 2016 The Authors. Haemophilia Published by John Wiley & Sons Ltd.

  9. Influence of clinical and laboratory variables on faecal antigen ELISA results in dogs with canine parvovirus infection.

    Science.gov (United States)

    Proksch, A L; Unterer, S; Speck, S; Truyen, U; Hartmann, K

    2015-06-01

    False negative faecal canine parvovirus (CPV) antigen ELISA results in dogs with CPV infection are common, but the factors that lead to these false negative results are still unknown. The aim of this study was to investigate whether dogs with a false negative faecal CPV antigen ELISA result have milder clinical signs and laboratory changes, a lower faecal virus load, higher faecal and serum CPV antibody titres and a faster recovery than dogs with a positive result. Eighty dogs with CPV infection, confirmed by the presence of clinical signs and a positive faecal CPV polymerase chain reaction (PCR), were assigned to two groups according to their faecal antigen ELISA result. Time until presentation, severity of symptoms, laboratory parameters, faecal virus load, faecal and serum antibody titres, and CPV sequencing data were compared between both groups. In 38/80 dogs that were hospitalised until recovery, the time to recovery, mortality, and the course of the disease were compared between dogs with positive and negative faecal antigen ELISA results. Of the 80 dogs included, 41 (51.3%) had a false negative faecal antigen ELISA result. ELISA-negative dogs had a significantly shorter time until presentation, lower frequency of defaecation, lower faecal virus load, and higher serum antibody concentrations than ELISA-positive dogs. Laboratory changes, CPV shedding, and outcomes were not associated with faecal antigen ELISA results. In conclusion, low faecal CPV load and antibodies binding to CPV antigen in faeces are likely to be important reasons for false negative faecal antigen ELISA results. Dogs with clinical signs of CPV infection should be retested by faecal PCR.

  10. Results of Single-Event Latchup Measurements Conducted by the Jet Propulsion Laboratory

    Science.gov (United States)

    Irom, Farokh; Miyahira, Tetsuo F.

    2010-01-01

    This paper reports recent single-event latchup (SEL) results for a variety of microelectronic devices that include OpAmp, Voltage Reference, Motor Controller, Switch Mode Controller, Resolver-to-Digital Converter and Analog-to-Digital Converter. The data was collected to evaluate these devices for possible use in NASA spacecraft.

  11. The USAF Phillips Laboratory sodium-sulfur battery technology program: Results and status

    Science.gov (United States)

    Rainbow, Marc E.; Somerville, Andrew

    1996-01-01

    Tests performed on NaS batteries are reported. The results of safety and abuse testing, shock and vibration tests, cell failure on warm-up, freeze thaw, overtemperature conditions, electrolyte fracture, overdischarge, and short circuit tests are presented along with GEO and LEO cycle tests and the status of the NaS cell flight tests.

  12. Enhancements in Glovebox Design Resulting from Laboratory-Conducted FIre Tests

    Energy Technology Data Exchange (ETDEWEB)

    Brooks, Kriston P.; Wunderlich, Gregory M.; Mcentire, James R.; Richmond, William G.

    2013-06-14

    The primary mission of the Pit Disassembly and Conversion Facility (PDCF) Project was to disassemble nuclear weapons pits and convert the resulting special nuclear materials to a form suitable for further disposition. Because of the nature of materials involved, the fundamental system which allowed PDCF to perform its mission was a series of integrated and interconnected gloveboxes which provided confinement and containment of the radioactive materials being processed. The high throughput planned for PDCF and the relatively high neutron and gamma radiation levels of the pits required that gloveboxes be shielded to meet worker dose limits. The glovebox shielding material was required to contain high hydrogen concentrations which typically result in these materials being combustible. High combustible loadings created design challenges for the facility fire suppression and ventilation system design. Combustible loading estimates for the PDCF Plutonium (Pu) Processing Building increased significantly due to these shielding requirements. As a result, the estimates of combustible loading substantially exceeded values used to support fire and facility safety analyses. To ensure a valid basis for combustible loading contributed by the glovebox system, the PDCF Project funded a series of fire tests conducted by the Southwest Research Institute on door panels and a representative glovebox containing Water Extended Polyester (WEP) radiological shielding to observe their behavior during a fire event. Improvements to PDCF glovebox designs were implemented based on lessons learned during the fire test. In particular, methods were developed to provide high levels of neutron shielding while maintaining combustible loading in the glovebox shells at low levels. Additionally, the fire test results led to design modifications to mitigate pressure increases observed during the fire test in order to maintain the integrity of the WEP cladding. These changes resulted in significantly

  13. Results of S5 CH tests with an ARL (Aerospace Research Laboratory) cascade

    Science.gov (United States)

    Fourmaux, A.

    1984-01-01

    As part of a research agreement between Iowa State University of Science and Technology (ISU) and ONERA, a blade cascade designed and already tested in the United States was manufactured and tested in France. The ONERA cascade wind tunnel and mock-up are described. Attention is focused on defining the upstream conditions of the cascade. Experimental results are presented, as well as comparisons with tests conducted by Detroit Diesel Allison (DDA) in the United States.

  14. Field and laboratory testing for amitraz-tolerant varroa populations. How comparable are their results?

    OpenAIRE

    Pires, Sância; Pereira, Óscar; Murilhas, António

    2007-01-01

    Portuguese beekeepers have frequently used amitraz (Apivar, Acadrex) in an attempt to cope with Varroa. As a result of beekeepers’ claims of an alarming decrease in amitraz field treatment efficacy, a nation-wide screening project was launched to ascertain the merit of those claims and, eventually, introduce changes in the way state institutions were interacting with beekeeping associations and beekeepers. Approximately 4.000 colonies were field-tested (following a similar approach to the ...

  15. Analysis and Treatment of Abnormal Chromatographic Result Fault in 220kV Main Transformer%一台220kV主变色谱异常故障的分析及处理

    Institute of Scientific and Technical Information of China (English)

    孔祥凤; 李进

    2012-01-01

    The abnormal chromatographic result in 220kV main transformer is analyzed. The fault position is diagnosed based on electric test results.%对220kV主变色谱异常结果进行了分析,结合电气试验对故障的位置进行了判断。

  16. Area Monitoring Dosimeter Program for the Pacific Northwest National Laboratory: Results for CY 1999

    Energy Technology Data Exchange (ETDEWEB)

    Bivins, Steven R.; Stoetzel, Gregory A.

    2000-09-19

    In January 1993, PNNL established an area monitoring dosimeter program in accordance with Article 514 of the DOE Radiological Control Manual. This program was to minimize the number of areas requiring issuance of personnel dosimeters and to demonstrate that doses outside Radiological Buffer Areas are negligible. In accordance with 10 CFR Part 835.402 (a)(1)-(4) and Article 511.1 of the DOE Standard Radiological Control, personnel dosimetry shall be provided to 1) radiological workers who are likely to receive at least 100 mrem annually and 2) declared pregnant workers, minors, and members of the public who are likely to receive at least 50 mrem annually. Program results for calendar years 1993-1998 confirmed that personnel dosimetry was not needed for individuals located in areas monitored by the program. A total of 123 area thermoluminescent dosimeters (TLDs) were placed in PNNL facilities during calendar year 1999. The TLDs were exchanged and analyzed quarterly. All routine area monitoring TLD results were less than 50 mrem annually after correcting for worker occupancy. The results support the conclusion that personnel dosimeters are not necessary for staff, declared pregnant workers, minors, or members of the public in these monitored areas.

  17. Database dictionary for the results of groundwater tracer tests using tritiated water, conducted at the Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, B.K. [Univ. of Tennessee, Knoxville, TN (United States). Dept. of Civil Engineering; Huff, D.D. [Oak Ridge National Lab., TN (United States). Environmental Sciences Div.

    1997-05-01

    In 1977, the United States Geological Survey (USGS) conducted two tracer tests at the Oak Ridge National Laboratory (ORNL) using tritiated water to study the relative importance of bedding-plane openings on shallow groundwater flow. Through a cooperative agreement between the USGS and the US Department of Energy (DOE), the data were made available to researchers at the Oak Ridge National Laboratory (ORNL), who organized the data into a data management format. The results of these groundwater tracer tests have been compiled into a collection of four SAS data sets. This report documents these SAS data sets, including their structure, methodology, and content. The SAS data sets include information on precipitation, tritium, water levels, and well construction for wells at or near ORNL radioactive waste burial grounds 4, 5, and 6.

  18. The Titan Haze Simulation Experiment: Latest Laboratory Results and Dedicated Plasma Chemistry Model

    Science.gov (United States)

    Sciamma-O'Brien, Ella; Raymond, Alexander; Mazur, Eric; Salama, Farid

    2017-06-01

    In Titan’s atmosphere, a complex organic chemistry occurs between its main constituents, N2 and CH4, and leads to the production of larger molecules and solid aerosols.Here, we present the latest results on the gas and solid phase analyses in the Titan Haze Simulation (THS) experiment, developed on the NASA Ames COSmIC simulation chamber. The THS is a unique experimental platform that allows us to simulate Titan’s atmospheric chemistry at Titan-like temperature (200K) by cooling down N2-CH4-based mixtures in a supersonic expansion before inducing the chemistry by plasma. Because of the accelerated gas flow in the expansion, the residence time of the gas in the active plasma region is less than 3 µs. This results in a truncated chemistry that enables us to monitor the first and intermediate steps of the chemistry as well as specific chemical pathways when adding, in the initial gas mixture, heavier molecules that have been detected as trace elements on Titan[1].We discuss the results of recent Mid-infrared (MIR) spectroscopy[2] and X-ray Absorption Near Edge Structure spectroscopy studies of THS Titan tholins produced in different gas mixtures (with and without acetylene and benzene). Both studies have shown the presence of nitrogen chemistry, and differences in the level and nature of the nitrogen incorporation depending on the initial gas mixture. A comparison of THS MIR spectra to VIMS data has shown that the THS aerosols produced in simpler mixtures, i.e., that contain more nitrogen and where the N-incorporation is in isocyanide-type molecules instead of nitriles, are more representative of Titan’s aerosols.In addition, a new model has been developed to simulate the plasma chemistry in the THS. Electron impact and chemical kinetics equations for more than 120 species are followed. The calculated mass spectra are in good agreement with the experimental THS mass spectra[1], confirming that the short residence time in the plasma cavity limits the growth of

  19. Distribution of Rare Earth Metals in Technogenic Wastes of Energy Enterprises (Results of the Laboratory Studies)

    OpenAIRE

    Alexandr Ivanovich Khanchuk; Aleksandr Alekseevich Yudakov; Mikhail Azaryevich Medkov; Leonid Nikolayevich Alekseyko; Andrey Vasilyevich Taskin; Sergey Igorevich Ivannikov

    2016-01-01

    The results of the research interaction between ash and slag samples from Vladivostok TPP’s landfills saturated with underburning and ammonium hydrodifluoride were given. It was found out that the reactions of the main components of a concentrate with NH4HF2 are flowing with creation of complex ammonium fluoro-metalate. It is shown that the distribution of REM (rare earth metals) between foam and heavier products is going during the flotation process of carbon-containing ash and slag samples ...

  20. Clinical results of stereotactic helium-ion radiosurgery of the pituitary gland at Lawrence Berkeley Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Levy, R.P.; Fabrikant, J.I.; Lyman, J.T.; Frankel, K.A.; Phillips, M.H.; Lawrence, J.H.; Tobias, C.A.

    1989-12-01

    The first therapeutic clinical trial using accelerated heavy-charged particles in humans was performed for the treatment of various endocrine and metabolic disorders of the pituitary gland, and as suppressive therapy for adenohypophyseal hormone-responsive carcinomas and diabetic retinopathy. Since then, over 800 patients have received stereotactically-directed plateau-beam heavy-charged particle pituitary irradiation at this institution. In acromegaly, Cushing's disease, Nelson's syndrome and prolactin-secreting tumors, the therapeutic goal in the 433 patients treated has been to destroy or inhibit the growth of the pituitary tumor and control hormonal hypersecretion, while preserving a functional rim of tissue with normal hormone-secreting capacity, and minimizing neurologic injury. An additional group of 34 patients was treated for nonsecreting chromophobe adenomas. This paper discusses the methods and results of these treatments. 11 refs.

  1. Distribution of Rare Earth Metals in Technogenic Wastes of Energy Enterprises (Results of the Laboratory Studies

    Directory of Open Access Journals (Sweden)

    Alexandr Ivanovich Khanchuk

    2016-12-01

    Full Text Available The results of the research interaction between ash and slag samples from Vladivostok TPP’s landfills saturated with underburning and ammonium hydrodifluoride were given. It was found out that the reactions of the main components of a concentrate with NH4HF2 are flowing with creation of complex ammonium fluoro-metalate. It is shown that the distribution of REM (rare earth metals between foam and heavier products is going during the flotation process of carbon-containing ash and slag samples without significant concentrating. It is shown that the water leaching of fluoridated product lets transfer silicone, aluminum and iron salts into solution and concentrate rare earth elements in insoluble residue in the form of complex salts of NaLnF4 general formula. We propose a schematic diagram of hydrodifluoride recycling of carbon-containing sample, which provides concentrating of REM with incomplete separation of macro-components.

  2. Description of buffer tests in 2005 - 2007. Results of laboratory tests

    Energy Technology Data Exchange (ETDEWEB)

    Sanden, Torbjoern; Boergesson, Lennart; Dueck, Ann; Goudarzi, Reza; Loennqvist, Margareta; Nilsson, Ulf; Aakesson, Mattias (Clay Technology AB, Lund (Sweden))

    2008-12-15

    The horizontal design for the deposition of nuclear waste in granitic rock has been ongoing since 2002. Clay Technology has contributed with studies that mainly concern the behaviour and design of the bentonite buffer material. The work described in this report was a part of the design subproject and was conducted from 2005 up to mid-2007. The results of the work and the increased general understanding of the behaviour of the buffer in KBS-3H have led to two main designs. BD (Basic Design) and DAWE (Drainage Artificial Watering and air Evacuation). Several significant uncertainties related to the behaviour of distance blocks and buffer materials were identified. The most important issues to be resolved were included in an extensive buffer test plan and this report presents the work carried. The critical issues (an issue is defined as critical if there is clear uncertainty in fulfilling the design basis) to be resolved to produce viable designs were: 1. Humidity-induced swelling. This process may cause cracking and subsequent loss of bentonite as the debris falls on to the floor. There is also the possibility that the blocks could swell and come into contact with the rock wall. Both these processes will lead to a hindering of the free water flow on the tunnel floor in DAWE and may subsequently result in the erosion of bentonite material from the tunnel. This is not expected to be an issue in the BD owing to the small buffer-rock gap engineered into the design. 2. Erosion of of filling blocks and buffer. This process will lead either to a loss of material from the emplacement drift if it takes place before a hydraulic plug is built or to redistribution of bentonite in the emplacement drift if it takes place afterwards. Localized erosion may be harmful for both design alternatives if it results in a substantial loss or redistribution of material. 3. Artificial wetting of distance blocks. Both design alternatives include artificial water filling of the gap between the

  3. Abnormal Uterine Bleeding FAQ

    Science.gov (United States)

    ... FREQUENTLY ASKED QUESTIONS FAQ095 GYNECOLOGIC PROBLEMS Abnormal Uterine Bleeding • What is a normal menstrual cycle? • When is bleeding abnormal? • At what ages is abnormal bleeding more ...

  4. Chromosome abnormalities in Indonesian patients with short stature

    Directory of Open Access Journals (Sweden)

    Paramayuda Chrysantine

    2012-08-01

    Full Text Available Abstract Background Short stature is associated with several disorders including wide variations of chromosomal disorders and single gene disorders. The objective of this report is to present the cytogenetic findings in Indonesian patients with short stature. Methods G-banding and interphase/metaphase FISH were performed on short stature patients with and without other clinical features who were referred by clinicians all over Indonesia to our laboratory during the year 2003–2009. Results The results of chromosomal analysis of ninety seven patients (mean age: 10.7 years old were collected. The group of patients with other clinical features showed sex chromosome abnormalities in 45% (18/40 and autosomal abnormalities in 10% (4/40, whereas those with short stature only, 42.1% (24/57 had sex chromosome abnormalities and 1.75% (1/57 had autosomal abnormalities. The autosomal chromosomal abnormalities involved mostly subtelomeric regions. Results discrepancies between karyotype and FISH were found in 10 patients, including detection of low-level monosomy X mosaicism in 6 patients with normal karyotype, and detection of mosaic aneuploidy chromosome 18 in 1 patient with 45,XX,rob(13;14(q10;q10. Statistical analysis showed no significant association between the groups and the type of chromosomal abnormalities. Conclusion Chromosome abnormalities account for about 50% of the short stature patients. Wide variations of both sex and autosomal chromosomes abnormalities were detected in the study. Since three out of five patients had autosomal structural abnormalities involving the subtelomeric regions, thus in the future, subtelomeric FISH or even a more sensitive method such as genomic/SNP microarray is needed to confirm deletions of subtelomeric regions of chromosome 9, 11 and 18. Low-level mosaicism in normal karyotype patients indicates interphase FISH need to be routinely carried out in short stature patients as an adjunct to karyotyping.

  5. Abnormalities of gonadal differentiation.

    Science.gov (United States)

    Berkovitz, G D; Seeherunvong, T

    1998-04-01

    Gonadal differentiation involves a complex interplay of developmental pathways. The sex determining region Y (SRY) gene plays a key role in testis determination, but its interaction with other genes is less well understood. Abnormalities of gonadal differentiation result in a range of clinical problems. 46,XY complete gonadal dysgenesis is defined by an absence of testis determination. Subjects have female external genitalia and come to clinical attention because of delayed puberty. Individuals with 46,XY partial gonadal dysgenesis usually present in the newborn period for the valuation of ambiguous genitalia. Gonadal histology always shows an abnormality of seminiferous tubule formation. A diagnosis of 46,XY true hermaphroditism is made if the gonads contain well-formed testicular and ovarian elements. Despite the pivotal role of the SRY gene in testis development, mutations of SRY are unusual in subjects with a 46,XY karyotype and abnormal gonadal development. 46,XX maleness is defined by testis determination in an individual with a 46,XX karyotype. Most affected individuals have a phenotype similar to that of Klinefelter syndrome. In contrast, subjects with 46,XX true hermaphroditism usually present with ambiguous genitalia. The majority of subjects with 46,XX maleness have Y sequences including SRY in genomic DNA. However, only rare subjects with 46,XX true hermaphroditism have translocated sequences encoding SRY. Mosaicism and chimaerism involving the Y chromosome can also be associated with abnormal gonadal development. However, the vast majority of subjects with 45,X/46,XY mosaicism have normal testes and normal male external genitalia.

  6. Heap leach studies on the removal of uranium from soil. Report of laboratory-scale test results

    Energy Technology Data Exchange (ETDEWEB)

    Turney, W.R.J.R.; York, D.A.; Mason, C.F.V.; Chisholm-Brause, C.J.; Dander, D.C.; Longmire, P.A.; Morris, D.E.; Strait, R.K.; Brewer, J.S.

    1994-05-01

    This report details the initial results of laboratory-scale testing of heap leach that is being developed as a method for removing uranium from uranium-contaminated soil. The soil used was obtained from the site of the Feed Materials Production Center (FMPC) near the village of Fernald in Ohio. The testing is being conducted on a laboratory scale, but it is intended that this methodology will eventually be enlarged to field scale where, millions of cubic meters of uranium-contaminated soil can be remediated. The laboratory scale experiments show that, using carbonate/bicarbonate solutions, uranium can be effectively removed from the soil from initial values of around 600 ppM down to 100 ppM or less. The goal of this research is to selectively remove uranium from the contaminated soil, without causing serious changes in the characteristics of the soil. It is also hoped that the new technologies developed for soil remediation at FEMP will be transferred to other sites that also have uranium-contaminated soil.

  7. Analysis of environmental contamination resulting from catastrophic incidents: part 1. Building and sustaining capacity in laboratory networks.

    Science.gov (United States)

    Magnuson, Matthew; Ernst, Hiba; Griggs, John; Fitz-James, Schatzi; Mapp, Latisha; Mullins, Marissa; Nichols, Tonya; Shah, Sanjiv; Smith, Terry; Hedrick, Elizabeth

    2014-11-01

    Catastrophic incidents, such as natural disasters, terrorist attacks, and industrial accidents, can occur suddenly and have high impact. However, they often occur at such a low frequency and in unpredictable locations that planning for the management of the consequences of a catastrophe can be difficult. For those catastrophes that result in the release of contaminants, the ability to analyze environmental samples is critical and contributes to the resilience of affected communities. Analyses of environmental samples are needed to make appropriate decisions about the course of action to restore the area affected by the contamination. Environmental samples range from soil, water, and air to vegetation, building materials, and debris. In addition, processes used to decontaminate any of these matrices may also generate wastewater and other materials that require analyses to determine the best course for proper disposal. This paper summarizes activities and programs the United States Environmental Protection Agency (USEPA) has implemented to ensure capability and capacity for the analysis of contaminated environmental samples following catastrophic incidents. USEPA's focus has been on building capability for a wide variety of contaminant classes and on ensuring national laboratory capacity for potential surges in the numbers of samples that could quickly exhaust the resources of local communities. USEPA's efforts have been designed to ensure a strong and resilient laboratory infrastructure in the United States to support communities as they respond to contamination incidents of any magnitude. The efforts include not only addressing technical issues related to the best-available methods for chemical, biological, and radiological contaminants, but also include addressing the challenges of coordination and administration of an efficient and effective response. Laboratory networks designed for responding to large scale contamination incidents can be sustained by applying

  8. Stroke prevention strategies in patients with atrial fibrillation and heart valve abnormalities: perceptions of 'valvular' atrial fibrillation: results of the European Heart Rhythm Association Survey.

    Science.gov (United States)

    Potpara, Tatjana S; Lip, Gregory Y H; Larsen, Torben B; Madrid, Antonio; Dobreanu, Dan; Jędrzejczyk-Patej, Ewa; Dagres, Nikolaos

    2016-10-01

    The purpose of this European Heart Rhythm Association (EHRA) Survey was to assess the perceptions of 'valvular' atrial fibrillation (AF) and management of AF patients with various heart valve abnormalities in daily clinical practice in European electrophysiology (EP) centres. Questionnaire survey was sent via the Internet to the EHRA-EP Research Network Centres. Of the 52 responding centres, 42 (80.8%) were university hospitals. Choosing the most comprehensive definition of valvular AF, a total of 49 centres (94.2%) encountered a mechanical prosthetic heart valve and significant rheumatic mitral stenosis, 35 centres (67.3%) also considered bioprosthetic valves, and 25 centres (48.1%) included any significant valvular heart disease, requiring surgical repair in the definition of valvular AF. Only three centres (5.8%) would define valvular AF as the presence of any (even mild) valvular abnormality. None of the centres would use non-vitamin K antagonist oral anticoagulants (NOACs) in AF patients with mechanical prosthetic valves, only 5 centres (9.8%) would use NOACs in patients with significant mitral stenosis, 17 centres (32.7%) would consider the use of NOACs in patients with bioprosthetic valves, and 21 centres (41.2%) would use NOACs in patients with a non-recent transcatheter valve replacement/implantation, while 13 centres (25.5%) would never consider the use of NOACs in AF patients with even mild native heart valve abnormality. Our survey showed marked heterogeneity in the definition of valvular AF and thromboprophylactic treatments, with the use of variable NOACs in patients with valvular heart disease other than prosthetic heart valves or significant mitral stenosis, indicating that this term may be misleading and should not be used.

  9. Sandia National Laboratories results for the 2010 criticality accident dosimetry exercise, at the CALIBAN reactor, CEA Valduc France.

    Energy Technology Data Exchange (ETDEWEB)

    Ward, Dann C.

    2011-09-01

    This document describes the personal nuclear accident dosimeter (PNAD) used by Sandia National Laboratories (SNL) and presents PNAD dosimetry results obtained during the Nuclear Accident Dosimeter Intercomparison Study held 20-23 September, 2010, at CEA Valduc, France. SNL PNADs were exposed in two separate irradiations from the CALIBAN reactor. Biases for reported neutron doses ranged from -15% to +0.4% with an average bias of -7.7%. PNADs were also exposed on the back side of phantoms to assess orientation effects.

  10. First trimester Down's syndrome screening shows high detection rate for trisomy 21, but poor performance in structural abnormalities--regional outcome results.

    Science.gov (United States)

    Rissanen, Anne; Niemimaa, Marko; Suonpää, Mikko; Ryynänen, Markku; Heinonen, Seppo

    2007-01-01

    To evaluate whether first trimester screening markers are altered in pregnancies affected both by other chromosomal defects than trisomy 21 and structural anomalies and whether it is possible to detect these pregnancies by combined ultrasound and biochemical screening test. Altogether 4,776 singleton pregnancies underwent first trimester screening. Of them, 3,101 women were screened using a combination of maternal serum free hCG, pregnancy-associated plasma protein A and nuchal translucency and 1,361 women with first trimester biochemistry without ultrasound. Nuchal translucency screening was performed between the 11th and 13+6th gestational weeks, and biochemistry 1-2 weeks earlier. Using a fixed cut-off rate of 1:250 for Down's syndrome, the detection rate of trisomies 21, 18 and 13 were 92, 67 and 0%, respectively. All open defects, 85% of cardiac defects and other minor defects were not detected in first trimester screening. Majority of these structural abnormalities occurred in women under 35 years of age. First trimester Down's syndrome screening is effective in trisomy screening, but its performance in structural abnormalities is low, when used as a part of routine clinical practice. We conclude that it is too early to drop second trimester screening ultrasound entirely from antenatal care programs if a high detection rate is to be achieved also in structural defects.

  11. The study of the karyotype analysis results of 358 couples with abnormal pregnancy history%358例不良孕产史夫妇的染色体核型分析

    Institute of Scientific and Technical Information of China (English)

    应香朵; 程启航

    2011-01-01

    目的 探讨具有不良孕产史夫妇的不良孕产史与染色体核型异常的关系.方法 采用外周血淋巴细胞培养技术,对358例具有不良孕产史的夫妇进行常规G显带核型分析.结果 358例不良孕产史夫妇中,共检出异常染色体核型39例,异常率为10.89%.其中随体变异13例,9号染色体臂间倒位9例,副缢痕的增长8例,相互易位7例,数目异常2例.染色体异常在男女发生的比例相当.结论 染色体核型异常是导致不良孕产史的重要原因之一,对不良孕产史夫妇双方进行细胞遗传学检查,提供优生咨询,再孕指导与监测,能够有效防止患儿出生,提高出生人口素质.%Objective: To investigate the relationship of abnormal pregnancy history and karyotype abnormality in 358 couples.Methods: The G - banding Patterns of the Chromosomes in 358 couples with abnormal pregnancy history were studied by Culture of Peripheral Blood Lymphocytes. Results: 39 karyotype abnormalities were detected in 358 couples, abnomal karyotype rate was 10. 89%.Among 39 cases, 13 had chromosome satellile varaiations, 9 had pericentric inversion 9, 8 had extended secondary constriction, 7 had balanced translocations, 2 had abnormal chromosome number. The abnomal karyotype rate was same between men and women.Conclusion: The karyotype abnormality is one of the important reasons of abnormal pregnancy. Cytogenetical study, eugenic advice,pregnancy guide and monitoring for couples with abnormal pregnancy history is useful to population quality.

  12. Historical return on investment and improved quality resulting from development and mining of a hospital laboratory relational database.

    Science.gov (United States)

    Brimhall, Bradley B; Hall, Timothy E; Walczak, Steven

    2006-01-01

    A hospital laboratory relational database, developed over eight years, has demonstrated significant cost savings and a substantial financial return on investment (ROI). In addition, the database has been used to measurably improve laboratory operations and the quality of patient care.

  13. Scoring life insurance applicants' laboratory results, blood pressure and build to predict all-cause mortality risk.

    Science.gov (United States)

    Fulks, Michael; Stout, Robert L; Dolan, Vera F

    2012-01-01

    Evaluate the degree of medium to longer term mortality prediction possible from a scoring system covering all laboratory testing used for life insurance applicants, as well as blood pressure and build measurements. Using the results of testing for life insurance applicants who reported a Social Security number in conjunction with the Social Security Death Master File, the mortality associated with each test result was defined by age and sex. The individual mortality scores for each test were combined for each individual and a composite mortality risk score was developed. This score was then tested against the insurance applicant dataset to evaluate its ability to discriminate risk across age and sex. The composite risk score was highly predictive of all-cause mortality risk in a linear manner from the best to worst quintile of scores in a nearly identical fashion for each sex and decade of age. Laboratory studies, blood pressure and build from life insurance applicants can be used to create scoring that predicts all-cause mortality across age and sex. Such an approach may hold promise for preventative health screening as well.

  14. Results from an inter-laboratory comparison of pneumococcal serotype-specific IgG measurement and critical parameters that affect assay performance.

    Science.gov (United States)

    Balloch, A; Licciardi, P V; Leach, A; Nurkka, A; Tang, M L K

    2010-02-01

    Quantitation of specific IgG to polysaccharides (serotypes) of Streptococcus pneumoniae provides the basis for evaluating vaccine efficacy. Different enzyme-linked immunosorbent assay (ELISA) methods are used internationally, making comparisons between laboratories difficult. We undertook an inter-laboratory comparison between two international laboratories performing serotype-specific IgG ELISAs using a panel of well-characterized serum samples: the Murdoch Childrens Research Institute Pneumococcal Laboratory (Melbourne, Australia) and the Vaccine Immunology Laboratory, National Public Health Institute (Helsinki, Finland). While good agreement was found for the inter-laboratory comparison for most serotypes, differences in ELISA methodology influenced specific IgG measurement. Therefore, use of the World Health Organization (WHO)-based ELISA methods for measurement of serotype-specific IgG is reliable, accurate and provides consistent results between international laboratories.

  15. Laboratory Tests

    Science.gov (United States)

    Laboratory tests check a sample of your blood, urine, or body tissues. A technician or your doctor ... compare your results to results from previous tests. Laboratory tests are often part of a routine checkup ...

  16. Early Recognition and Treatment of Sepsis After the Addition of Lactate to the Laboratory's Critical Result Call List.

    Science.gov (United States)

    Kuttab, Hani I; Sterk, Ethan; Rech, Megan A; Nghiem, Trac; Bahar, Burak; Kahn, Stephen

    2016-09-11

    Screening of patients with sepsis is needed to increase recognition and allow for earlier interventions. There is no consensus on whether the addition of lactate to the critical result laboratory's call list should be a standard practice. This was a retrospective cohort study that compared management and outcomes of patients with sepsis having lactate ≥4 mmol/L before (group 1) and after (group 2) the addition of a critical result threshold of lactate of ≥4 mmol/L to the critical result laboratory's call list and its effects on time to antibiotics and intravenous fluids (IVFs). One hundred twenty-one patients were included. Lactate was higher in group 1 (7.0 ± 4.3 vs 5.6 ± 2.0, P = 0.03). More patients in group 2 received hydrocortisone (1.9% vs 22.4%, P = .001). Hospital mortality, 30-day mortality, and 90-day mortality were significantly lower in group 2 (59.3% vs 32.8%, P = .003; 68.5% vs 37.3%, P ≤ .001; 68.5% vs 41.8%, P = .002). There were no significant differences in total volume of IVFs (2400.8 ± 1720.0 vs 2483.7 ± 2155.7, P = 0.83), time to start IVFs (184.0 ± 283.2 vs 115.6 ± 190.5, P = 0.27), or antibiotics (184.8 ± 187.1 vs 133.7 ± 137.4, P = 0.16). Addition of lactate to the critical result laboratory's call list did not lead to a statistically significant improvement in time to IVFs or antibiotics, although the average time to antibiotics and IVFs decreased by 51.1 and 68.4 minutes, respectively. Hospital mortality, 30-day mortality, and 90-day mortality were lower in group 2, which may be, in part, due to increased recognition of severe sepsis by critical result notification and earlier intervention. © The Author(s) 2016.

  17. Evaluation of Tuberculosis Laboratory results in Çanakkale Onsekiz Mart University Research and Education Hospital for 2009-2011

    Directory of Open Access Journals (Sweden)

    Nilgun ÖZBEY

    2012-08-01

    Full Text Available Objective: Tuberculosis microbiological laboratorydiagnosis was firstly started in year 2009, in MicrobiologyLaboratory of Onsekiz Mart University Research andEducation Hospital in Çanakkale. We aimed at thisstudy to present our laboratory data and to evaluatethe methods which were used for the diagnosis ofmicobacteria.Method: Samples sent to our laboratory fortuberculosis culture were stained by Ehrlich-Ziehl-Neelsen (EZN method and evaluated microscopically.After processing of samples, each sample was inoculatedto Löwenstein-Jensen medium (LJ and BACTEC MGIT960 (Mycobacteria Growth Indicator Tube, BectonDickinson, USA liquid based medium. If suspectedgrowth was detected, Mycobacterium tuberculosiscomplex (MTBC typing was made and if requestedantituberculosis drug susceptibility for streptomycin(STR, isoniazid (INH, rifampicin (RF and ethambutol(ETM tested. Samples from normally sterile body sitescultured directly, others were firstly decontaminatedand concentrated.Results: During the study period 1.048 samplesfrom 667 patient has been processed. Seventy eightsamples (7.44% from 54 patients were found positive by BACTEC MGIT system: 71 of them MTBC and sevenof them were mycobacteria other than tuberculosis(MOTT. By LJ medium 64 MTBC and 4 MOTT strain,totally 68 mycobacterium were isolated. Mean timefor detecting positive culture by MGIT 960 was 11.8 days(± 7.45 SD. With EZN stain, 49 samples were detectedas acido resistant bacilli and only 42 (86% of them werepositive by culture. Antituberculosis drug susceptibilty wasevaluated at isolates of 25 from 54 patients. A resistanceto at least one of the drugs were detected in six isolates.It is found that five isolates were resistant to STR, threewere resistant to INH and one was resistant to ETM. Threeisolates were resistant to both STR and INH. Rifampicinresistance was not detected in MTBC.Conclusion: With this study we presented firsttuberculosis laboratory findings from our province

  18. Evaluation of Outcome- Prenatal Diagnosis Indication and Results Suitability in Families Referred to our Laboratory For Prenatal Diagnosis

    Directory of Open Access Journals (Sweden)

    Ayşegül Türkyılmaz

    2007-01-01

    Full Text Available Since our aim is to establish the importance, necessity and concept of prenatal diagnosis in our region and supply routine service at a stage which we admit as a transitional period for application, all of the materials of amniocentesis, cordocentesis and corion villi sample referred to laboratories were evaluated without refusal.When we examined prenatal diagnoses of these specimens, we found Down Risk (according to triple test result in 164 specimens (%34, fetal anomaly risk in 122 (%25, advanced age in 69 (%14 poor-obstetric anamnesis in 27(%5, Down Syndrome- infant history in 20 (%4, family request in 17, and habitual abortus (%3 etc. in specimens. Lymphocyte Culture prepared in duplicate for each specimen and chromosome were obtained from total of ten slides for each specimen. Slides were stained with Giemsa Banding Technic (GTG Banding. Total (10x481 4810 slides were evaluated for diagnosis.There were no false positive and false negative results.

  19. EDiFiSE full-FPGA adaptive optics: first laboratory results using the IACAT optical ground support equipment

    Science.gov (United States)

    Chulani, Haresh M.; Martín, Yolanda; Fuensalida, Jesús J.; Rodríguez-Ramos, Luis F.; Echeandía, Carlos; Puga, Marta; Alonso, Angel

    2016-07-01

    This paper reviews the EDiFiSE (Equalized and Diffraction-limited Field Spectrograph Experiment) full-FPGA (Field Programmable Gate Array) adaptive optics (AO) system and presents its first laboratory results. EDiFiSE is a prototype equalized integral field unit (EIFU) spectrograph for the observation of high-contrast systems in the Willian Herschel Telescope (WHT). Its AO system comprises two independent parallel full-FPGA control loops, one for tip-tilt and one for higher order aberrations. Xilinx's Virtex-4 and Virtex-5 FPGA's fixed point arithmetic and their interfacing with the rest of the AO components and the user have been adequately dealt with, and a very deterministic system with a negligible computational delay has been obtained. The AO system has been recently integrated in laboratory and verified using the IACAT (IAC Atmosphere and Telescope) optical ground support equipment. Closed loop correction bandwidths of 65 Hz for the tip-tilt and 25 Hz for higher order aberrations are obtained. The system has been tested in the visible range for the WHT with a 9 x 9 subpupil configuration, low star magnitude, wind speeds up to 10 m/s and Fried parameter down to 18 cm, and a resolution below the EIFU's fiber section has been obtained.

  20. Elevated blood lead in California adults, 1987: results of a statewide surveillance program based on laboratory reports.

    Science.gov (United States)

    Maizlish, N; Rudolph, L; Sutton, P; Jones, J R; Kizer, K W

    1990-08-01

    California medical laboratories that test for blood lead are required to report results exceeding 1.21 mumols/L (25 micrograms/dl). Between April and December 1987, the California Department of Health Services received 3,077 blood lead reports from 34 laboratories for 1,293 civilian, non-institutionalized adults. Approximately 1 percent of all reports exceeded 3.87 mumols/L (80 micrograms/dl), 7 percent exceeded 2.42 mumols/L (50 micrograms/dl), and 21 percent exceeded 1.93 mumols/L (40 micrograms/dl). Individuals tested were overwhelmingly male (94 percent), disproportionately Hispanic surnamed (44 percent), and most often residents of Los Angeles County (81 percent). Workers in lead smelting, battery manufacturing, and brass foundries accounted for nearly 80 percent of reports. Construction, radiator repair, pottery and ceramics manufacturing, and gun firing ranges accounted for the remainder. All adults with reports of greater than or equal to 2.90 mumols/L who were contacted reported on occupational exposure. Approximately half were not in routine medical monitoring programs. Despite OSHA standards, elevated blood lead with the potential for serious acute and chronic lead poisoning in California adults remains a significant public health and major occupational health concern.

  1. Use of laboratory tests to guide initiation of autologous hematopoietic progenitor cell collection by apheresis: results from the multicenter hematopoietic progenitor cell collection by Apheresis Laboratory Trigger Survey.

    Science.gov (United States)

    Makar, Robert S; Padmanabhan, Anand; Kim, Haewon C; Anderson, Christina; Sugrue, Michele W; Linenberger, Michael

    2014-10-01

    Limited literature describes the value of laboratory "triggers" to guide collection of peripheral blood (PB) hematopoietic progenitor cells (HPCs) by apheresis [HPC(A)]. We used a web-based survey to determine which parameters are used to initiate autologous HPC(A) collection in adult and pediatric patients and to identify common practice patterns. Members of the AABB Cellular Therapy Product Collection and Clinical Practices Subsection and the American Society for Apheresis HPC Donor Subcommittee drafted and developed relevant survey questions. A web link to the survey was distributed by electronic newsletter or email. Responses from 67 programs that perform autologous HPC(A) collections, including academic medical centers (n = 46), blood centers (n = 10), community hospitals (n = 5), and a variety of other medical institutions (n = 6), were analyzed. Ninety-three percent (62/67) of programs used a laboratory parameter to initiate HPC(A) collection. In both adult (40/54, 74%) and pediatric (29/38, 76%) patients, the PB CD34+ cell count was the most common parameter used to initiate HPC(A) collection. The median PB CD34+ trigger value was 10/μL for both patient populations. Among centers routinely using the PB CD34+ cell count to initiate apheresis, 51% (22/43) first sent the test before the patient presented for collection. Although more than 90% of centers used a laboratory test to trigger apheresis in cytokine-mobilized (44/48) or chemomobilized patients (50/53), only 57% (30/53) used a laboratory trigger if the patient was mobilized with granulocyte colony-stimulating factor plus plerixafor. Forty-two percent (21/50) of programs that routinely measured the PB CD34+ count before collection and discontinued further HPC(A) collection based on product CD34+ cell yield also stopped if the PB CD34+ value before apheresis was considered too low to proceed. Most programs use the PB CD34+ cell count to trigger autologous HPC(A) collection. Some centers also use this

  2. Supplementing claims data with outpatient laboratory test results to improve confounding adjustment in effectiveness studies of lipid-lowering treatments

    Directory of Open Access Journals (Sweden)

    Schneeweiss Sebastian

    2012-11-01

    Full Text Available Abstract Background Adjusting for laboratory test results may result in better confounding control when added to administrative claims data in the study of treatment effects. However, missing values can arise through several mechanisms. Methods We studied the relationship between availability of outpatient lab test results, lab values, and patient and system characteristics in a large healthcare database using LDL, HDL, and HbA1c in a cohort of initiators of statins or Vytorin (ezetimibe & simvastatin as examples. Results Among 703,484 patients 68% had at least one lab test performed in the 6 months before treatment. Performing an LDL test was negatively associated with several patient characteristics, including recent hospitalization (OR = 0.32, 95% CI: 0.29-0.34, MI (OR = 0.77, 95% CI: 0.69-0.85, or carotid revascularization (OR = 0.37, 95% CI: 0.25-0.53. Patient demographics, diagnoses, and procedures predicted well who would have a lab test performed (AUC = 0.89 to 0.93. Among those with test results available claims data explained only 14% of variation. Conclusions In a claims database linked with outpatient lab test results, we found that lab tests are performed selectively corresponding to current treatment guidelines. Poor ability to predict lab values and the high proportion of missingness reduces the added value of lab tests for effectiveness research in this setting.

  3. Statistical comparison of the results from six analytical chemistry laboratories of the mercury content of muscle tissue of two species of sharks.

    Science.gov (United States)

    Walker, T I

    1977-01-01

    Statistical tests were carried out on the results of chemical analysis for total mercury concentrations of replicate samples of muscle tissue of school shark Galeorhinus australis (Macleay) and gummy shark Mustelus antarcticus Guenther from six independent analytical laboratories. These tests showed that one laboratory produced results 9% below the overall average of all results, another 1% below average while the other four were all 5% above average. Moreover, one laboratory had significantly lower scatter of results than the others, and the percentage scatter (standard error expressed as a percentage of the mean) in two of the laboratories tended to diminish as the magnitude of the results increased. Correction for what were concluded to be wild points indicated that the scatter for all laboratories was below 14%.

  4. Statistical comparison of the results from six analytical chemistry laboratories of the mercury content of muscle tissue of two species of sharks

    Energy Technology Data Exchange (ETDEWEB)

    Walker, T.I.

    1977-01-01

    Statistical tests were carried out on the results of chemical analysis for total mercury concentrations of replicate samples of muscle tissue of school shark Galeorhinus australis (Macleay) and gummy shark Mustelus antarcticus Guenther from six independent analytical laboratories. These tests showed that one laboratory produced results 9% below the overall average of all results, another 1% below average while the other four were all 5% above average. Moreover, one laboratory had significantly lower scatter of results than the others, and the percentage scatter (standard error expressed as a percentage of the mean) in two of the laboratories tended to diminish as the magnitude of the results increased. Correction for what were concluded to be wild points indicated that the scatter for all laboratories was below 14%.

  5. [REPORTING CRITICAL LAB RESULTS, A CHALLENGE FOR THE LAB AND THE PHYSICIAN - A SUMMARY OF FOUR YEARS OF EXPERIENCE IN MEIR MEDICAL CENTER LABORATORIES].

    Science.gov (United States)

    Rashid, Gloria; Goldman, Jacob; Weinstein, Doron; Tohami, Tali; Neumark, Eran; Weiss, Eli

    2015-08-01

    Critical laboratory results require prompt reporting to the attending physician, as they may indicate that a patient is in a life-threatening condition. Although this important subject has been covered in many publications, it needs more attention from our healthcare organizations, which have no official policy on the subject. Matching expectations between the doctor and the laboratory needs to be better defined. The aim of this work was to inform the community of doctors and laboratories about the multiple problems concerning the reporting of critical laboratory results, to create a platform for exchanging views and ideas, and to build an extensive infrastructure for developing a unified plan to address this important issue. We present the results of four years of experience of reporting critical laboratory values at the Meir Medical Center Laboratories. The idea leading this work was to present the relatively low rate of critical results reported by the laboratories in 2010, sharing the problems discovered while investigating the situation in depth, and presenting the solutions that enabled us to obtain the desired results within four years. Gradual implementation of these improvements resulted in critical value reporting increasing from 55% in 2010 to 95% currently. We suggest a model for improving critical laboratory values reporting based on our 4-year experience, which emphasizes: (1) The importance of selecting proper tests and values for critical results; (2) The significance of using technology and computerized measures to support the process; and (3) Developing quick procedures for monitoring and controlling the process.

  6. Results and Perspectives for Laboratory Search of Weakly Interacting Sub-eV Particles with the OSQAR Experiment

    CERN Document Server

    Pugnat, P.; Schott, M.; Husek, T.; Sulc, M.; Deferne, G.; Duvillaret, L.; Finger, M., Jr.; Finger, M.; Flekova, L.; Hosek, J.; Jary, V.; Jost, R.; Kral, M.; Kunc, S.; Macuchova, K.; Meissner, K.A.; Morville, J.; Romanini, D.; Siemko, A.; Slunecka, M.; Vitrant, G.; Zicha, J.

    2014-01-01

    Recent intensive theoretical and experimental studies highlight the possibility of new fundamental particle physics beyond the standard model that can be probed by sub-eV energy experiments. The OSQAR photon regeneration experiment looks for Light Shining through a Wall (LSW) from the quantum oscillation of optical photons into Weakly Interacting Sub-eV Particles (WISPs), like axion or axion-like particles (ALPs), in a 9 T transverse magnetic field over the unprecedented length of 2 x 14.3 m. No excess of events has been detected over the background. The di-photon couplings of possible new light scalar and pseudo-scalar particles can be constrained in the massless limit to be less than 8.0 x 10-8 GeV-1. These results are very close to the most stringent laboratory constraints obtained for the coupling of WISPs to two photons. Plans for further improving the sensitivity of the OSQAR experiment are presented.

  7. Design and preliminary test results of the 40 MW power supply at the National High Magnetic Field Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Boenig, H.J. [Los Alamos National Lab., NM (United States); Bogdan, F.; Morris, G.C. [ABB Drives Inc., New Berlin, WI (United States); Ferner, J.A.; Schneider-Muntau, H.J. [National High Magnetic Field Lab., Tallahassee, FL (United States); Rumrill, R.H.; Rumrill, R.S. [Alpha Scientific Electronics Inc., Hayward, CA (United States)

    1993-11-01

    Four highly stabilized, steady-state, 10 MW power supplies have been installed at the National High Magnetic Field Laboratory in Tallahassee, FL. Each supply consists of a 12.5 kV vacuum circuit breaker, two three-winding, step-down transformers, a 24-pulse rectifier with interphase reactors and freewheeling diodes, and a passive and an active filter. Two different transformer tap settings allow dc supply output voltages of 400 and 500 V. The rated current of a supply is 17 kA and each supply has a one hour overload capability of 20 kA. The power supply output bus system, including a reversing switch at the input and 2 {times} 16 disconnect switches at the output, connects each supply to 16 different magnet cells. The design of the power supply is described and preliminary test results with a supply feeding a 10 MW resistive load are presented.

  8. Laboratory testing results of kinetics and processing technology of the polymetallic sulphide concentrate Blagojev Kamen – Serbia

    Directory of Open Access Journals (Sweden)

    Milorad Ćirković

    2016-06-01

    Full Text Available This work presents the laboratory testing results of kinetics the oxidation process and sample processing of the sulphide polymetallic concentrate Blagojev Kamen. The aim of investigation is recovery of these types of raw material, present in large quantities in the peripheral parts of already used primary mineral deposits of copper, because of their high economic potential due to the content of a large number of metals and especially precious metals. Characterization of this raw material is based on the chemical analyses, XRD results, DTA analysis, etc. For these investigations, the sulphide concentrate with the following content was used in %: Cu – 2.3; Fe – 19.8; S – 27.19; Zn – 9.13; As – 0.167; Pb – 15.63; SiO2 – 17.93; CaO – 0.97; Al2O3 – 1.43; Ag – 480 g/t; Au – 659 g/t. Kinetic investigations of oxidation processes were carried out under the isothermal conditions within the temperature range of 400 to 625 oC. The Sharp's model was used for determination the kinetics parameters, and determined values of activation energy are 67 kJ/mole for the first period, and 47 kJ/mole for the second period. Pyrometallurgical treatment of this type of polymetallic concentrate, in the laboratory conditions, was carried out using the oxidative roasting and, then the reduction smelting was done in the Taman's furnace. Gold from 90.5 to 97.95% and silver from 77.28 to 93.37% are moved into the raw lead (smelting product. Gold from 1.1 to 3.92% and silver from 4.35 to 8.42% are moved into the polymetallic copper matte. Gold from 0.58 to 1.6% and silver from 2.45 to 6.82% are moved into the slag.

  9. Zero-Gravity Atmospheric Cloud Physics Experiment Laboratory engineering concepts/design tradeoffs. Volume 1: Study results

    Science.gov (United States)

    Greco, R. V.; Eaton, L. R.; Wilkinson, H. C.

    1974-01-01

    The work is summarized which was accomplished from January 1974 to October 1974 for the Zero-Gravity Atmospheric Cloud Physics Laboratory. The definition and development of an atmospheric cloud physics laboratory and the selection and delineation of candidate experiments that require the unique environment of zero gravity or near zero gravity are reported. The experiment program and the laboratory concept for a Spacelab payload to perform cloud microphysics research are defined. This multimission laboratory is planned to be available to the entire scientific community to utilize in furthering the basic understanding of cloud microphysical processes and phenomenon, thereby contributing to improved weather prediction and ultimately to provide beneficial weather control and modification.

  10. Genetic antimicrobial susceptibility testing in Gram-negative sepsis - impact on time to results in a routine laboratory.

    Science.gov (United States)

    Kommedal, Øyvind; Aasen, Johanne Lind; Lindemann, Paul Christoffer

    2016-07-01

    Diagnostic testing of positive blood cultures is among the most critical tasks performed by clinical microbiology laboratories, and the total analysis time from sampling to results should be kept as short as possible. By providing identification of pelleted bacteria directly from positive blood-cultures, MALDI-TOF MS opens for relatively low-complex species-adjusted genetic susceptibility testing from the same bacterial pellet. In our lab routine, we prospectively evaluated a rapid in-house real-time PCR targeting the most common aminoglycoside and cephalosporin resistance genes in Escherichia coli and Klebsiella pneumoniae and measured time to preliminary susceptibility reporting for 138 samples. The results were compared to direct phenotypic susceptibility testing with interpretation after 6 h and overnight incubation respectively. Results from the genetic susceptibility testing were available for 69.5% (96/138) of the positive blood cultures within 24 h after sample collection. No phenotypic susceptibility results were available at this time. Compared to overnight direct susceptibility testing, the average time from sample collection to preliminary susceptibility reporting was reduced with 43%, from 45 h and 5 min to 25 h and 44 min, providing an earlier adjustment of antimicrobial therapy for 12 patients. Minor logistic adjustments have the potential to save yet another 4 h.

  11. Night traffic and health: Results of laboratory and field studies; Naechtlicher Verkehrslaerm und Gesundheit: Ergebnisse von Labor- und Feldstudien

    Energy Technology Data Exchange (ETDEWEB)

    Maschke, C. [Technische Univ. Berlin (Germany). Inst. fuer Technische Akustik; Ising, H. [Umweltbundesamt, Berlin (Germany). Inst. fuer Wasser-, Boden-, und Lufthygiene; Arndt, D. [Robert Koch-Institut, Medizinische Diagnostik - Umweltmedizin, Berlin (Germany)

    1995-04-01

    The present article summarizes the results obtained in the Berlin laboratory and field studies on `noise-induced stress reactions`. Unaccustomed air-traffic noise at night leads to elevated epinephrine and cortisol levels. If road traffic noise at night rises above the accustomed level, this leads to enhanced renal cortisol secretion. Noise at night deserves to be termed a stressor because it is able to activate both the hypothalamic-pituitary-adrenocortical and the hypothalamic-adrenomedullar control loop. Elevation of epinephrine secretion was more distinct in the air-traffic field study than it was in the laboratory study. The relative elevation of cortisol secretion in the field study was of the same order as that of epinephrine secretion. Noise-induced noctural elevation of epinephrine secretion seems only to occur in the case of unaccustomed noise levels. If exposure to noise is prolonged, it subsides in an adaptive process and gives way to an elevated cortisol secretion. Elevation of both cortisol and epinephrine secretion is indicative of a stressing traffic noise situation. Air-traffic noise was found to impair subjective sleep quality and lead to a rise in epinephrine secretion both in the laboratory and the field study. No correlation was found between noise emission from low-flying aircraft and epinephrine or cortisol secretion in the field study. Neither the field nor the laboratory study showed a correlation between the number of night flights and catecholamine secretion. Cortisol secretion remained equally unaffected by the number of flights. The present results confirm the hypothesis that air and road traffic noise cause bodily distress. Chronic exposure to noise at night must therfore be regarded as a health hazard. (orig./MG) [Deutsch] Ungewohnter naechtlicher Fluglaerm fuehrt zu einer erhoehten Adrenalin-und Cortisolausscheidung. Wird die Intensitaet eines gewohnten naechtlichen Strassenverkehrslaerms erhoeht, ist eine vermehrte renale

  12. CO2 efflux from a calcareous Mojave Desert soil: isotopic results from a laboratory and field study

    Science.gov (United States)

    Verburg, P.; Stevenson, B.

    2011-12-01

    Soil inorganic carbon (SIC) represents a substantial C pool in arid ecosystems. The contribution of the SIC pool to net ecosystem C flux is poorly understood but has gained attention because there have been reports of anomalous C fluxes in some arid environments. In the context of climate change, altered precipitation patterns and changes in soil pCO2 values (from changes in vegetation density, plant water use efficiency, and belowground respiration) could potentially affect SIC storage in some ecosystems. The stable carbon isotope values of organic and inorganic carbon (e.g. carbonates) can differ substantially and may be useful in determining whether PIC influences C fluxes. However, variable rates of heterotrophic and root respiration and diffusion of atmospheric CO2 into the soil as well as the variation and complexity of the CaCO3-CO2-H2O system at different soil depths can complicate interpretation of isotopic data. We monitored soil CO2 concentrations and CO2 efflux from irrigated and non-irrigated plots in a calcareous soil at the Mojave Global Change Facility (MGCF). The site is on the northern part of the Mojave Desert with a mean annual precipitation of 71 mm and vegetation characterized by a Larrea tridentata, Lycium spp., Ambrosia dumosa plant community. We used a Keeling plot approach to determine source δ13C values from effluxed CO2-13C in a laboratory incubation experiment and from direct field measurements of soil CO2-13C. Data from the laboratory incubation experiment suggested that there was a contribution of PIC on effluxed CO2-13C in a closed system, but results from the field measurements were much more difficult to interpret and did not support a large contribution of SIC to CO2 fluxes in these soils. We discuss the usefulness of isotopic measure of CO2 on CO2 efflux in the context of the MGCF experiment.

  13. Results of the eurofoods trial on between-laboratory variation in the analysis of macronutrients in foods

    NARCIS (Netherlands)

    Hollman, Peter C. H.; Katan, M.B.

    1987-01-01

    In order to determine the influence of laboratory procedures on nutrient values in different food tables and data banks, an interlaboratory study was set up. Nineteen laboratories participated, and received well-homogenized samples of egg powder, full-fat milk powder, whole rye and wheat meal,

  14. Results of the Eurofood trials on between-laboratory variation in the analysis of macronutrient in foods.

    NARCIS (Netherlands)

    Hollman, P.C.H.; Katan, M.B.

    1987-01-01

    In order to determine the influence of laboratory procedures on nutrient values in different food tables and data banks, an interlaboratory study was set up. Nineteen laboratories participated, and received well-homogenized samples of egg powder, full-fat milk powder, whole rye and wheat meal, biscu

  15. [Influenza surveillance in nine consecutive seasons, 2003-2012: results from National Influenza Reference Laboratory, Istanbul Faculty Of Medicine, Turkey].

    Science.gov (United States)

    Akçay Ciblak, Meral; Kanturvardar Tütenyurd, Melis; Asar, Serkan; Tulunoğlu, Merve; Fındıkçı, Nurcihan; Badur, Selim

    2012-10-01

    Influenza is a public health problem that affects 5-20% of the world population annually causing high morbidity and mortality especially in risk groups. In addition to determining prevention and treatment strategies with vaccines and antivirals, surveillance data plays an important role in combat against influenza. Surveillance provides valuable data on characteristics of influenza activity, on types, sub-types, antigenic properties and antiviral resistance profile of circulating viruses in a given region. The first influenza surveillance was initiated as a pilot study in 2003 by now named National Influenza Reference Laboratory, Istanbul Faculty of Medicine. Surveillance was launched at national level by Ministry of Health in 2004 and two National Influenza Laboratories, one in Istanbul and the other in Ankara, have been conducting surveillance in Turkey. Surveillance data obtained for nine consecutive years, 2003-2012, by National Influenza Reference Laboratory in Istanbul Faculty of Medicine have been summarized in this report. During 2003-2012 influenza surveillance seasons, a total of 11.077 nasal swabs collected in viral transport medium were sent to the National Influenza Reference Laboratory, Istanbul for analysis. Immun-capture ELISA followed by MDCK cell culture was used for detection of influenza viruses before 2009 and real-time RT-PCR was used thereafter. Antigenic characterizations were done by hemagglutination inhibition assay with the reactives supplied by World Health Organization. Analysis of the results showed that influenza B viruses have entered the circulation in 2005-2006 seasons, and have contributed to the epidemics at increasing rates every year except in the 2009 pandemic season. Influenza B Victoria and Yamagata lineages were cocirculating for two seasons. For other seasons either lineage was in circulation. Antigenic characterization revealed that circulating B viruses matched the vaccine composition either partially or totally for only

  16. Abnormal Uterine Bleeding

    Science.gov (United States)

    ... first few months of a normal pregnancy. Some birth control pills or the intrauterine device (IUD) can also cause ... this type can significantly reduce abnormal bleeding. Like birth control pills, sometimes IUDs can actually cause abnormal bleeding. Tell ...

  17. Urine - abnormal color

    Science.gov (United States)

    ... medlineplus.gov/ency/article/003139.htm Urine - abnormal color To use the sharing features on this page, please enable JavaScript. The usual color of urine is straw-yellow. Abnormally colored urine ...

  18. A comparison of results from two mycology laboratories for the diagnosis of onychomycosis: a study of 85 cases in a geriatric population.

    Science.gov (United States)

    Scherer, William P; Scherer, Michael D

    2004-01-01

    An investigative study was performed to compare the results from two mycology laboratories for the diagnosis of onychomycosis in a geriatric population and to determine the possible pharmacologic treatments based on the two laboratories' results. In this study, 85 cases of suspected onychomycosis involving men and women 65 years and older from a nursing home setting in South Florida were used. Samples were taken from the hallux toenail and sent to two different mycology laboratories for fluorescent potassium hydroxide preparation and microscopic examination of a fungal culture. Of the 85 cases studied, the two mycology laboratories reported similar potassium hydroxide preparation results for 58.8% of the patients and similar fungal culture results for genus and species identification for 37.6% of the patients. When the potassium hydroxide preparation and fungal culture results were combined, the two mycology laboratories reported similar results for only 27.1% of the patients. As a result of the two mycology laboratories' findings, the possible US Food and Drug Administration-approved pharmacologic treatments may differ for 43.5% of the patients studied. The discrepancy between the two independent laboratories leaves physicians to question the reproducibility of fluorescent potassium hydroxide preparation and fungal culture analysis in a geriatric patient population for the diagnosis of onychomycosis.

  19. On Regularity of Abnormal Subriemannian Geodesics

    CERN Document Server

    Tan, Kanghai

    2012-01-01

    We prove the smoothness of abnormal minimizers of subriemannian manifolds of step 3 with a nilpotent basis. We prove that rank 2 Carnot groups of step 4 admit no strictly abnormal minimizers. For any subriemannian manifolds of step less than 7, we show all abnormal minimizers have no corner type singularities, which partly generalize the main result of Leonardi-Monti.

  20. First results from the DarkSide-50 dark matter experiment at Laboratori Nazionali del Gran Sasso

    Energy Technology Data Exchange (ETDEWEB)

    Agnes, P.; Alexander, T.; Alton, A.; Arisaka, K.; Back, H. O.; Baldin, B.; Biery, K.; Bonfini, G.; Bossa, M.; Brigatti, A.; Brodsky, J.; Budano, F.; Cadonati, L.; Calaprice, F.; Canci, N.; Candela, A.; Cao, H.; Cariello, M.; Cavalcante, P.; Chavarria, A.; Chepurnov, A.; Cocco, A. G.; Crippa, L.; D' Angelo, D.; D' Incecco, M.; Davini, S.; De Deo, M.; Derbin, A.; Devoto, A.; Di Eusanio, F.; Di Pietro, G.; Edkins, E.; Empl, A.; Fan, A.; Fiorillo, G.; Fomenko, K.; Forster, G.; Franco, D.; Gabriele, F.; Galbiati, C.; Goretti, A.; Grandi, L.; Gromov, M.; Guan, M. Y.; Guardincerri, Y.; Hackett, B.; Herner, K.; Hungerford, E. V.; Ianni, Al.; Ianni, An.; Jollet, C.; Keeter, K.; Kendziora, C.; Kidner, S.; Kobychev, V.; Koh, G.; Korablev, D.; Korga, G.; Kurlej, A.; Li, P. X.; Loer, B.; Lombardi, P.; Love, C.; Ludhova, L.; Luitz, S.; Ma, Y. Q.; Machulin, I.; Mandarano, A.; Mari, S.; Maricic, J.; Marini, L.; Martoff, C. J.; Meregaglia, A.; Meroni, E.; Meyers, P. D.; Milincic, R.; Montanari, D.; Monte, A.; Montuschi, M.; Monzani, M. E.; Mosteiro, P.; Mount, B.; Muratova, V.; Musico, P.; Nelson, A.; Odrowski, S.; Okounkova, M.; Orsini, M.; Ortica, F.; Pagani, L.; Pallavicini, M.; Pantic, E.; Papp, L.; Parmeggiano, S.; Parsells, R.; Pelczar, K.; Pelliccia, N.; Perasso, S.; Pocar, A.; Pordes, S.; Pugachev, D.; Qian, H.; Randle, K.; Ranucci, G.; Razeto, A.; Reinhold, B.; Renshaw, A.; Romani, A.; Rossi, B.; Rossi, N.; Rountree, S. D.; Sablone, D.; Saggese, P.; Saldanha, R.; Sands, W.; Sangiorgio, S.; Segreto, E.; Semenov, D.; Shields, E.; Skorokhvatov, M.; Smirnov, O.; Sotnikov, A.; Stanford, C.; Suvorov, Y.; Tartaglia, R.; Tatarowicz, J.; Testera, G.; Tonazzo, A.; Unzhakov, E.; Vogelaar, R. B.; Wada, M.; Walker, S.; Wang, H.; Wang, Y.; Watson, A.; Westerdale, S.; Wojcik, M.; Wright, A.; Xiang, X.; Xu, J.; Yang, C. G.; Yoo, J.; Zavatarelli, S.; Zec, A.; Zhu, C.; Zuzel, G.

    2015-04-01

    We report the first results of DarkSide-50, a direct search for dark matter operating in the underground Laboratori Nazionali del Gran Sasso (LNGS) and searching for the rare nuclear recoils possibly induced by weakly interacting massive particles (WIMPs). The dark matter detector is a Liquid Argon Time Projection Chamber with a (46.4±0.7) kg active mass, operated inside a 30 t organic liquid scintillator neutron veto, which is in turn installed at the center of a 1 kt water Cherenkov veto for the residual flux of cosmic rays. We report here the null results of a dark matter search for a View the MathML source exposure with an atmospheric argon fill. This is the most sensitive dark matter search performed with an argon target, corresponding to a 90% CL upper limit on the WIMP-nucleon spin-independent cross section of 6.1×10-44 cm2 for a WIMP mass of 100 Gev/c2

  1. First Results from the DarkSide-50 Dark Matter Experiment at Laboratori Nazionali del Gran Sasso

    CERN Document Server

    Agnes, P; Alton, A; Arisaka, K; Back, H O; Baldin, B; Biery, K; Bonfini, G; Bossa, M; Brigatti, A; Brodsky, J; Budano, F; Cadonati, L; Calaprice, F; Canci, N; Candela, A; Cao, H; Cariello, M; Cavalcante, P; Chavarria, A; Chepurnov, A; Cocco, A G; Crippa, L; D'Angelo, D; D'Incecco, M; Davini, S; De Deo, M; Derbin, A; Devoto, A; Di Eusanio, F; Di Pietro, G; Edkins, E; Empl, A; Fan, A; Fiorillo, G; Fomenko, K; Forster, G; Franco, D; Gabriele, F; Galbiati, C; Goretti, A; Grandi, L; Gromov, M; Guan, M Y; Guardincerri, Y; Hackett, B; Herner, K; Hungerford, E V; Ianni, Al; Ianni, An; Jollet, C; Keeter, K; Kendziora, C; Kidner, S; Kobychev, V; Koh, G; Korablev, D; Korga, G; Kurlej, A; Li, P X; Loer, B; Lombardi, P; Love, C; Ludhova, L; Luitz, S; Ma, Y Q; Machulin, I; Mandarano, A; Mari, S; Maricic, J; Marini, L; Martoff, C J; Meregaglia, A; Meroni, E; Meyers, P D; Milincic, R; Montanari, D; Montuschi, M; Monzani, M E; Mosteiro, P; Mount, B; Muratova, V; Musico, P; Nelson, A; Odrowski, S; Okounkova, M; Orsini, M; Ortica, F; Pagani, L; Pallavicini, M; Pantic, E; Papp, L; Parmeggiano, S; Parsells, R; Pelczar, K; Pelliccia, N; Perasso, S; Pocar, A; Pordes, S; Pugachev, D; Qian, H; Randle, K; Ranucci, G; Razeto, A; Reinhold, B; Renshaw, A; Romani, A; Rossi, B; Rossi, N; Rountree, S D; Sablone, D; Saggese, P; Saldanha, R; Sands, W; Sangiorgio, S; Segreto, E; Semenov, D; Shields, E; Skorokhvatov, M; Smirnov, O; Sotnikov, A; Stanford, C; Suvorov, Y; Tartaglia, R; Tatarowicz, J; Testera, G; Tonazzo, A; Unzhakov, E; Vogelaar, R B; Wada, M; Walker, S; Wang, H; Wang, Y; Watson, A; Westerdale, S; Wojcik, M; Wright, A; Xiang, X; Xu, J; Yang, C G; Yoo, J; Zavatarelli, S; Zec, A; Zhu, C; Zuzel, G

    2015-01-01

    We report the first results of DarkSide-50, a direct search for dark matter operating in the underground Laboratori Nazionali del Gran Sasso (LNGS) and searching for the rare nuclear recoils possibly induced by weakly interacting massive particles (WIMPs). The dark matter detector is a Liquid Argon Time Projection Chamber with a (46.4+-0.7) kg active mass, operated inside a 30 t organic liquid scintillator neutron veto, which is in turn installed at the center of a 1 kt water Cherenkov veto for the residual flux of cosmic rays. We report here the null results of a dark matter search for a (1422+-67) kg d exposure with an atmospheric argon fill. This is the most sensitive dark matter search performed with an argon target, corresponding to a 90% CL upper limit on the WIMP-nucleon spin-independent cross section of 6.1x10^-44 cm^2 for a WIMP mass of 100 GeV/c^2.

  2. First results from the DarkSide-50 dark matter experiment at Laboratori Nazionali del Gran Sasso

    Directory of Open Access Journals (Sweden)

    P. Agnes

    2015-04-01

    Full Text Available We report the first results of DarkSide-50, a direct search for dark matter operating in the underground Laboratori Nazionali del Gran Sasso (LNGS and searching for the rare nuclear recoils possibly induced by weakly interacting massive particles (WIMPs. The dark matter detector is a Liquid Argon Time Projection Chamber with a (46.4±0.7 kg active mass, operated inside a 30 t organic liquid scintillator neutron veto, which is in turn installed at the center of a 1 kt water Cherenkov veto for the residual flux of cosmic rays. We report here the null results of a dark matter search for a (1422±67 kgd exposure with an atmospheric argon fill. This is the most sensitive dark matter search performed with an argon target, corresponding to a 90% CL upper limit on the WIMP-nucleon spin-independent cross section of 6.1×10−44 cm2 for a WIMP mass of 100 Gev/c2.

  3. Chromosomal abnormalities in spontaneous abortion after assisted reproductive treatment

    Directory of Open Access Journals (Sweden)

    Kim You

    2010-11-01

    Full Text Available Abstract Background We evaluated cytogenetic results occurring with first trimester pregnancy loss, and assessed the type and frequency of chromosomal abnormalities after assisted reproductive treatment (ART and compared them with a control group. We also compared the rate of chromosomal abnormalities according to infertility causes in ICSI group. Methods A retrospective cohort analysis was made of all patients who were referred to the Genetics Laboratory of Fertility Center of CHA Gangnam Medical Center from 2005 to 2009 because of clinical abortion with a subsequent dilation and evacuation (D&E performed, and patients were grouped by type of conception as follows: conventional IVF (in vitro fertilization (n = 114, ICSI (intracytoplasmic sperm injection (n = 140, and control (natural conception or intrauterine insemination [IUI] (n = 128. Statistical analysis was performed using SPSS software. Results A total 406 specimens were referred to laboratory, ten abortuses were excluded, and in 14 cases, we did not get any spontaneous metaphase, chromosomal constitutions of 382 specimens were successfully obtained with conventional cytogenetic methods. Overall, 52.62% of the miscarriages were found to be cytogenetically abnormal among all patients, the frequency was 48.4% in the control group, 54.3% of miscarriages after ICSI and 55.3% after conventional IVF (p = 0.503. The most prevalent abnormalities were autosomal trisomy, however, nine (11.69% sex chromosome aneuploidy were noted in the ICSI group vs. four (6.45% and two (3.23% cases in the conventional IVF group and control group. We compared chromosomal abnormalities of miscarriages after ICSI according to infertility factor. 55.71% underwent ICSI due to male factors, 44.29% due to non-male factors. ICSI group having male factors showed significantly higher risk of chromosomal abnormalities than ICSI group having non-male factors (65.8% vs. 34.2%, p = 0.009, odds ratio = 1.529, 95% CI = 1

  4. Accuracy evaluation of a CAS system: laboratory protocol and results with 6D localizers, and clinical experiences in otorhinolaryngology.

    Science.gov (United States)

    Schmerber, S; Chassat, F

    2001-01-01

    The objective of the study reported in this article was to evaluate (1) localizer inaccuracies, one of the major sources of errors in Computer-Assisted Surgery (CAS) systems, and (2) the final errors obtained using surface-based registration in ear, nose, and throat (ENT) surgery. These objectives were met through (1) a technical evaluation of the accuracy and usability of several optical localizers under laboratory test conditions, and (2) a clinical measure of the global errors obtained when using a CAS system including one of the standard localizer systems (Flashpoint 5000) in Functional Endoscopic Sinus Surgery (FESS). The technical evaluation of localizers consisted of series of geometric tests on four commercial systems. Clinical evaluation included the development of a laboratory CAS system using a markerless, skin surface registration method. This was based on a standard optical digitizing system (Flashpoint 5000), which eliminates the need for the second CT scan, which is normally performed specifically to process the position of the fiducial markers. Global accuracy was then evaluated on 20 patients by subjective and visual comparison when placing a calibrated pointer on anatomical landmarks. The results of the technical study indicate that the four commercial systems tested have levels of inaccuracy deemed acceptable for most CAS applications, including ENT surgery. The clinical study obtained a registration and calibration accuracy of less than 1.5 mm in 89.2% (SD = 0.20 mm) of the cases studied. Our markerless skin surface points registration method is reliable, and allows patient head movements during the procedure. The accuracy tests performed show that this type of system can be used for ENT surgery with satisfaction. CAS systems enable the surgeon to have a more thorough understanding of the complicated anatomy of paranasal sinuses, and may be especially helpful in revision surgery when normal anatomic landmarks are lacking. Further studies are

  5. Surfactant control of gas transfer velocity along an offshore coastal transect: results from a laboratory gas exchange tank

    Science.gov (United States)

    Pereira, R.; Schneider-Zapp, K.; Upstill-Goddard, R. C.

    2016-07-01

    Understanding the physical and biogeochemical controls of air-sea gas exchange is necessary for establishing biogeochemical models for predicting regional- and global-scale trace gas fluxes and feedbacks. To this end we report the results of experiments designed to constrain the effect of surfactants in the sea surface microlayer (SML) on the gas transfer velocity (kw; cm h-1), seasonally (2012-2013) along a 20 km coastal transect (North East UK). We measured total surfactant activity (SA), chromophoric dissolved organic matter (CDOM) and chlorophyll a (Chl a) in the SML and in sub-surface water (SSW) and we evaluated corresponding kw values using a custom-designed air-sea gas exchange tank. Temporal SA variability exceeded its spatial variability. Overall, SA varied 5-fold between all samples (0.08 to 0.38 mg L-1 T-X-100), being highest in the SML during summer. SML SA enrichment factors (EFs) relative to SSW were ˜ 1.0 to 1.9, except for two values (0.75; 0.89: February 2013). The range in corresponding k660 (kw for CO2 in seawater at 20 °C) was 6.8 to 22.0 cm h-1. The film factor R660 (the ratio of k660 for seawater to k660 for "clean", i.e. surfactant-free, laboratory water) was strongly correlated with SML SA (r ≥ 0.70, p ≤ 0.002, each n = 16). High SML SA typically corresponded to k660 suppressions ˜ 14 to 51 % relative to clean laboratory water, highlighting strong spatiotemporal gradients in gas exchange due to varying surfactant in these coastal waters. Such variability should be taken account of when evaluating marine trace gas sources and sinks. Total CDOM absorbance (250 to 450 nm), the CDOM spectral slope ratio (SR = S275 - 295/S350 - 400), the 250 : 365 nm CDOM absorption ratio (E2 : E3), and Chl a all indicated spatial and temporal signals in the quantity and composition of organic matter in the SML and SSW. This prompts us to hypothesise that spatiotemporal variation in R660 and its relationship with SA is a consequence of compositional

  6. Electrolytes assessed by point-of-care testing - Are the values comparable with results obtained from the central laboratory?

    Directory of Open Access Journals (Sweden)

    Binila Chacko

    2011-01-01

    Full Text Available Background and Aims: When dealing with very sick patients, the speed and accuracy of tests to detect metabolic derangements is very important. We evaluated if there was agreement between whole blood electrolytes measured by a point-of-care device and serum electrolytes measured using indirect ion-selective electrodes. Materials and Methods: In this prospective study, electrolytes were analyzed in 44 paired samples drawn from critically ill patients. Whole blood electrolytes were analyzed using a point-of-care blood gas analyzer and serum electrolytes were analyzed in the central laboratory on samples transported through a rapid transit pneumatic system. Agreement was summarized by the mean difference with 95% limits of agreement (LOA and Lin′s concordance correlation (p c. Results: There was a significant difference in the mean (±standard deviation sodium value between whole blood and serum samples (135.8 ± 5.7 mmol/L vs. 139.9 ± 5.4 mmol/L, P < 0.001, with the agreement being modest (p c = 0.71; mean difference -4.0; 95% LOA -8.78 to 0.65. Although the agreement between whole blood and serum potassium was good (p c = 0.96, and the average difference small (-0.3; 95% LOA -0.72 to 0.13, individual differences were clinically significant, particularly at lower potassium values. For potassium values <3.0 mmol/L, the concordance was low (p c = 0.53 and the LOA was wide (1.0 to -0.13. The concordance for potassium was good (p c = 0.96 for values ≥3.0 (mean difference -0.2; 95% LOA -0.48 to 0.06. Conclusions: Clinicians should be aware of the difference between whole blood and serum electrolytes, particularly when urgent samples are tested at point of care and routine follow-up electrolytes are sent to the central laboratory. A correction factor needs to be determined at each center.

  7. Results from the second year of operation of the Federal Methanol Fleet at Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    West, B.H.; McGill (Oak Ridge National Lab., TN (USA)); Hillis, S.L. (Tennessee Univ., Knoxville, TN (USA))

    1990-09-01

    The Oak Ridge National Laboratory has completed its second year of operation of ten vehicles for the Federal Methanol Fleet Project; five of the vehicles are fueled with methanol. Over 56,000 miles were accumulated on the vehicles in the second year bringing the total to over 152,000 miles. Energy consumption for the methanol cars was slightly higher than that of the gasoline cars again this year, most likely as a result of shorter average trip lengths for the methanol gas. Iron and lead have accumulated at greater rates in the lubricating oil of the methanol cars. Driver's ratings of vehicles reflected some dissatisfaction with the cold-weather performance of the methanol cars, but the cars have no special provisions for cold weather starting, and the fuel vapor pressure has not been tailored to the season as at other test sites. Otherwise, drivers' opinions of the methanol cars have been favorable. 13 refs., 4 figs., 10 tabs.

  8. Review of the Usefulness of Various Rotational Seismometers with Laboratory Results of Fibre-Optic Ones Tested for Engineering Applications

    Directory of Open Access Journals (Sweden)

    Leszek R. Jaroszewicz

    2016-12-01

    Full Text Available Starting with descriptions of rotational seismology, areas of interest and historical field measurements, the fundamental requirements for rotational seismometers for seismological and engineering application are formulated. On the above basis, a review of all existing rotational seismometers is presented with a description of the principles of their operation as well as possibilities to fulfill formulated requirements. This review includes mechanical, acoustical, electrochemical and optical devices and shows that the last of these types are the most promising. It is shown that optical rotational seismometer based on the ring-laser gyroscope concept is the best for seismological applications, whereas systems based on fiber-optic gyroscopes demonstrate parameters which are also required for engineering applications. Laboratory results of the Fibre-Optic System for Rotational Events & Phenomena Monitoring using a small 1-D shaking table modified to generate rotational excitations are presented. The harmonic and time-history tests demonstrate its usefulness for recording rotational motions with rates up to 0.25 rad/s.

  9. Changes in the Chemistry of Groundwater Reacted with CO2: Comparison of Laboratory Results with the ZERT Field Pilot

    Science.gov (United States)

    Kharaka, Yousif K.; Thordsen, James J.; Abedini, Atosa A.; Beers, Sarah; Thomas, Burt

    2017-01-01

    As part of the ZERT program, sediments from two wells at the ZERT site, located in Bozeman, Montana, USA were reacted with a solution having the composition of local groundwater. A total of 50 water samples were collected from 7 containers placed for 15 days in a glove box with one atmosphere of CO2 to investigate detailed changes in the concentrations of major, minor and trace inorganic compounds, and to compare these with changes observed in groundwater at the ZERT site following CO2 injection. Laboratory results included rapid changes in pH (8.6 to 5.7), alkalinity (243 to 1295 mg/L as HCO3), electrical conductance (539 to 1822 μS/cm), Ca (28 to 297 mg/L), Mg (18 to 63 mg/L), Fe (5 to 43 μg/L) and Mn (2 to 837 μg/L) following CO2 injection. These chemical changes, which are in general agreement with those obtained from sampling the ZERT monitoring wells, could provide early detection of CO2 leakage into shallow groundwater. Dissolution of calcite, some dolomite and minor Mn-oxides, and desorption/ion exchange are likely the main geochemical processes responsible for the observed changes.

  10. First X-Ray Diffraction Results from Mars Science Laboratory: Mineralogy of Rocknest Aeolian Bedform at Gale Crater

    Science.gov (United States)

    Bish, D. L.; Blake, D. F.; Vaniman, D. T.; Chipera, S. J.; Sarrazin, P.; Morris, R. V.; Ming, D. W.; Treiman, A. H.; Downs, R. T.; Morrison, S. M.; Yen, A. S.; Achilles, C. N.; Morookian, J. M.; Farmer, J. D.; Crisp, J. A.; Rampe, E. B.; Stolper, E. M.; DesMarais, D. J.; Spanovich, N.; Anderson, R. C.

    2013-01-01

    Numerous orbital and landed observations of the martian surface suggest a reasonably uniform martian soil composition, likely as a result of global aeolian mixing [1, 2]. Chemical data for martian soils are abundant [e.g., 2, 3], and phase information has been provided by lander thermal emission and Moessbauer spectroscopic measurements [3, 4, 5, 6]. However, until now no X-ray diffraction (XRD) data were available for martian soil nor has XRD ever been used on another body apart from Earth. XRD is generally considered the most definitive method for determining the crystalline phases in solid samples, and it is the method of choice for determining mineralogy. CheMin s first XRD analysis on Mars coincided with the 100th anniversary of the discovery of X-ray diffraction by von Laue. Curiosity delivered scooped samples of loose, unconsolidated material ("soil") acquired from an aeolian bedform at the Rocknest locality to instruments in the body of the rover (the laboratory). Imaging shows that the soil has a range of particle sizes, of 1-2 mm and smaller, presumably representing contributions from global, regional, and local sources.

  11. In situ vitrification demonstration at Pit 1, Oak Ridge National Laboratory. Volume 1: Results of treatability study

    Energy Technology Data Exchange (ETDEWEB)

    Spalding, B.P.; Naney, M.T.; Cline, S.R.; Bogle, M.A. [Oak Ridge National Lab., TN (United States). Environmental Sciences Div.; Tixier, J.S. [Pacific Northwest National Lab., Richland, WA (United States)

    1997-12-01

    A treatability study was initiated in October 1993 to apply in situ vitrification (ISV) to at least two segments of Oak Ridge National Laboratory (ORNL) seepage Pit 1 by the end of fiscal year (FY) 1995. This treatability study was later extended to include all of Pit 1 and was performed to support a possible Interim Record of Decision or removal action for closure of one or more of the seepage pits and trenches beginning as early as FY 1997. This treatability study was carried out to establish the field-scale technical performance of ISV for (1) attaining the required depth, nominally 15 ft, to incorporate source contamination within and beneath the pits; (2) demonstrating field capability for the overlap of melt settings which will be necessary to achieve fused, melted segments of the source contamination; (3) demonstrating off-gas handling technology for accommodating and minimizing the volatilization of {sup 137}Cs; (4) demonstrating adequate site characterization techniques to predict ISV melting kinetics, processing temperatures, and product durability; and (5) promoting public acceptance of ISV technology by demonstrating its safety, implementability, site impacts, and air emissions and by coordinating the treatability study within the regulatory closure process. In April 1996 an expulsion of an estimated 10% of the 196 Mg (216 tons) melt body occurred resulting in significant damage to ISV equipment and, ultimately, led to an indefinite suspension of further ISV operations at Pit 1. This report summarizes the technical accomplishments and status of the project in fulfilling these objectives through September 1997.

  12. Results of the Interlaboratory Exercise CSN/CIEMAT-02 Among Environmental Radioactivity Laboratories (Sea Fish); Resultados del Ejercicio Interlaboratorios de Radiactividad Ambiental CSN/CIEMAT-02 (Fauna Marina)

    Energy Technology Data Exchange (ETDEWEB)

    Romero gonzalez, M. L.

    2003-07-01

    The document describes the outcome of the CSN/CIEMAT-02 interlaboratory test comparison among environmental radioactivity laboratories. The exercise was organised according to the ISO-43 and the ISO/IUPAC/AOAC Harmonized Protocol for the proficiency testing of analytical laboratories. The test sample was a reference materials provided by the IAEA-MEL (IAE Marine Environmental Laboratory, Monaco), a sea fish containing environmental levels of U-238, U-234, K-40, Pb-210, Ra-226, Sr-90, Cs-137, Co-60, Pu-(239+240), Am-241 and Tc-99. The results of the exercise were computed for 32 participating laboratories, and their analytical performance was assessed using the z-score approach. A raised percentage of satisfactory laboratory performance has been obtained for all the analysis, being the best performance in gamma measurements. The laboratories have made an effort to calculate the combined uncertainty of the radiochemical determinations. Most of the laboratories have demonstrated its competence in performing the study analysis and also the adequate measuring capability of their detection equipment even in conditions close to detection limits. The study has shown the capacity of participant laboratories to perform radioactive determinations in environmental sea fish samples with satisfactory quality levels. (Author) 6 refs.

  13. Los Alamos Scientific Laboratory energy-related history, research, managerial reorganization proposals, actions taken, and results. History report, 1945--1979

    Energy Technology Data Exchange (ETDEWEB)

    Hammel, E.F.

    1997-03-01

    This report documents the development of major energy-related programs at the Los Alamos Scientific Laboratory between 1945 and 1979. Although the Laboratory`s primary mission during that era was the design and development of nuclear weapons and most of the Laboratory`s funding came from a single source, a number of factors were at work that led to the development of these other programs. Some of those factors were affected by the Laboratory`s internal management structure and organization; others were the result of increasing environmental awareness within the general population and the political consequences of that awareness; still others were related to the increasing demand for energy and the increasing turmoil in the energy-rich Middle East. This report also describes the various activities in Los Alamos, in Washington, and in other areas of the world that contributed to the development of major energy-related programs at Los Alamos. The author has a unique historical perspective because of his involvement as a scientist and manager at the Los Alamos Scientific Laboratory during the time period described within the report. In addition, in numerous footnotes and references, he cites a large body of documents that include the opinions and perspectives of many others who were involved at one time or another in these programs. Finally the report includes a detailed chronology of geopolitical events that led to the development of energy-related programs at Los Alamos.

  14. Cause analysis for unsatisfactory results in proficiency testing activities: a case study of Brazilian calibration laboratories accredited under ISO/IEC 17025:2005⋆

    Directory of Open Access Journals (Sweden)

    Silva M.A.F.

    2013-01-01

    Full Text Available This work presents the results of a survey carried out among Brazilian calibration laboratories accredited under ISO/IEC 17025:2005 with the objective to identify how these laboratories investigate the root causes of unsatisfactory results in proficiency testing. The survey was coordinated by the Brazilian accreditation body, the General Coordination for Accreditation (Cgcre, of the Institute of Metrology, Quality and Technology (Inmetro.

  15. Chromosomal abnormalities in spontaneous abortion after assisted reproductive treatment.

    Science.gov (United States)

    Kim, Ji Won; Lee, Woo Sik; Yoon, Tae Ki; Seok, Hyun Ha; Cho, Jung Hyun; Kim, You Shin; Lyu, Sang Woo; Shim, Sung Han

    2010-11-03

    We evaluated cytogenetic results occurring with first trimester pregnancy loss, and assessed the type and frequency of chromosomal abnormalities after assisted reproductive treatment (ART) and compared them with a control group. We also compared the rate of chromosomal abnormalities according to infertility causes in ICSI group. A retrospective cohort analysis was made of all patients who were referred to the Genetics Laboratory of Fertility Center of CHA Gangnam Medical Center from 2005 to 2009 because of clinical abortion with a subsequent dilation and evacuation (D&E) performed, and patients were grouped by type of conception as follows: conventional IVF (in vitro fertilization) (n = 114), ICSI (intracytoplasmic sperm injection) (n = 140), and control (natural conception or intrauterine insemination [IUI]) (n = 128). Statistical analysis was performed using SPSS software. A total 406 specimens were referred to laboratory, ten abortuses were excluded, and in 14 cases, we did not get any spontaneous metaphase, chromosomal constitutions of 382 specimens were successfully obtained with conventional cytogenetic methods. Overall, 52.62% of the miscarriages were found to be cytogenetically abnormal among all patients, the frequency was 48.4% in the control group, 54.3% of miscarriages after ICSI and 55.3% after conventional IVF (p = 0.503). The most prevalent abnormalities were autosomal trisomy, however, nine (11.69%) sex chromosome aneuploidy were noted in the ICSI group vs. four (6.45%) and two (3.23%) cases in the conventional IVF group and control group. We compared chromosomal abnormalities of miscarriages after ICSI according to infertility factor. 55.71% underwent ICSI due to male factors, 44.29% due to non-male factors. ICSI group having male factors showed significantly higher risk of chromosomal abnormalities than ICSI group having non-male factors (65.8% vs. 34.2%, p = 0.009, odds ratio = 1.529, 95% CI = 1.092-2.141). There is no increased risk of

  16. CHROMOSOMAL ABNORMALITIES IN PATIENTS WITH RECURRENT MISCARRIAGE

    Directory of Open Access Journals (Sweden)

    Daniela Mierla

    2012-06-01

    Full Text Available Chromosomal abnormalities are involved in the etiology of recurrent spontaneous pregnancy loss and sub-fertility. The purpose of this study was to determine the frequency and contribution of chromosomal abnormalities in recurrent miscarriages. The results obtained and literature review are helpful in understanding the importance of cytogenetics analysis of female infertility. To investigate the distribution of chromosomal abnormalities in the Romanian population with recurrent miscarriage, karyotype analysis by G-banding was performed from peripheral blood in 967 women infertility. Results: Chromosomal abnormalities were found to 79 women (8,17%. The percentage of chromosomal abnormalities in the studied population correlates with the data in the literature. Chromosomal abnormalities could play the important role in etiology of infertility and are more frequently detected in this group of patients compared to general population. In the infertile couples balanced chromosomal abnormalities are the main cause of spontaneous abortions.

  17. The DOSIS -Experiment onboard the Columbus Laboratory of the International Space Station -Overview and first mission results

    Science.gov (United States)

    Reitz, Guenther; Berger, Thomas; Kürner, Christine; Burmeister, Sünke; Hajek, Michael; Bilski, Pawel; Horwacik, Tomasz; Vanhavere, Filip; Spurny, Frantisek; Jadrnickova, Iva; Pálfalvi, József K.; O'Sullivan, Denis; Yasuda, Nakahiro; Uchihori, Yukio; Kitamura, Hisashi; Kodaira, Satoshi; Yukihara, Eduardo; Benton, Eric; Zapp, Neal; Gaza, Ramona; Zhou, Dazhuang; Semones, Edward; Roed, Yvonne; Boehme, Matthias; Haumann, Lutz

    Besides the effects of the microgravity environment, and the psychological and psychosocial problems encountered in confined spaces, radiation is the main health detriment for long dura-tion human space missions. The radiation environment encountered in space differs in nature from that on earth, consisting mostly of high energetic ions from protons up to iron, resulting in radiation levels far exceeding the ones encountered on earth for occupational radiation workers. Accurate knowledge of the physical characteristics of the space radiation field in dependence on the solar activity, the orbital parameters and the different shielding configurations of the International Space Station ISS is therefore needed. The DOSIS (Dose Distribution inside the ISS) experiment, under the project and science lead of DLR, aims for the spatial and tempo-ral measurement of the radiation field parameters inside the European Columbus laboratory onboard the International Space Station. This goal is achieved by applying a combination of passive (Thermo-and Optical luminescence detectors and Nuclear track etch detectors) and active (silicon telescope) radiation detectors. The passive radiation detectors -so called pas-sive detector packages (PDP) are mounted at eleven positions within the Columbus laboratory -aiming for a spatial dose distribution measurement of the absorbed dose, the linear energy transfer spectra and the dose equivalent with an average exposure time of six months. Two active silicon telescopes -so called Dosimetry Telescopes (DOSTEL 1 and DOSTEL 2) together with a Data and Power Unit (DDPU) are mounted within the DOSIS Main Box at a fixed loca-tion beneath the European Physiology Module (EPM) rack. The DOSTEL 1 and DOSTEL 2 detectors are positioned at a 90 angle to each other for a precise measurement of the temporal and spatial variation of the radiation field, especially during crossing of the South Atlantic Anomaly (SAA). The DOSIS hardware was launched with the

  18. LabPush: a pilot study of providing remote clinics with laboratory results via short message service (SMS in Swaziland, Africa.

    Directory of Open Access Journals (Sweden)

    Wen-Shan Jian

    Full Text Available BACKGROUND: Turnaround time (TAT is an important indicator of laboratory performance. It is often difficult to achieve fast TAT for blood tests conducted at clinics in developing countries. This is because clinics where the patient is treated are often far away from the laboratory, and transporting blood samples and test results between the two locations creates significant delay. Recent efforts have sought to mitigate this problem by using Short Message Service (SMS to reduce TAT. Studies reporting the impact of this technique have not been published in scientific literature however. In this paper we present a study of LabPush, a system developed to test whether SMS delivery of HIV related laboratory results to clinics could shorten TAT time significantly. METHOD: LapPush was implemented in six clinics of the Kingdom of Swaziland. SMS results were sent out from the laboratory as a supplement to normal transport of paper results. Each clinic was equipped with a mobile phone to receive SMS results. The laboratory that processes the blood tests was equipped with a system for digital input of results, and transmission of results via SMS to the clinics. RESULTS: Laboratory results were received for 1041 different clinical cases. The total number of SMS records received (1032 was higher than that of paper records (965, indicating a higher loss rate for paper records. A statistical comparison of TAT for SMS and paper reports indicates a statistically significant improvement for SMS. Results were more positive for more rural clinics, and an urban clinic with high workload. CONCLUSION: SMS can be used to reduce TAT for blood tests taken at clinics in developing countries. Benefits are likely to be greater at clinics that are further away from laboratories, due to the difficulties this imposes on transport of paper records.

  19. LabPush: A Pilot Study of Providing Remote Clinics with Laboratory Results via Short Message Service (SMS) in Swaziland, Africa

    Science.gov (United States)

    Jian, Wen-Shan; Hsu, Min-Huei; Sukati, Hosea; Syed-Abdul, Shabbir; Scholl, Jeremiah; Dube, Nduduzo; Hsu, Chun-Kung; Wu, Tai-jung; Lin, Vera; Chi, Tex; Chang, Peter; Li, Yu-Chuan

    2012-01-01

    Background Turnaround time (TAT) is an important indicator of laboratory performance. It is often difficult to achieve fast TAT for blood tests conducted at clinics in developing countries. This is because clinics where the patient is treated are often far away from the laboratory, and transporting blood samples and test results between the two locations creates significant delay. Recent efforts have sought to mitigate this problem by using Short Message Service (SMS) to reduce TAT. Studies reporting the impact of this technique have not been published in scientific literature however. In this paper we present a study of LabPush, a system developed to test whether SMS delivery of HIV related laboratory results to clinics could shorten TAT time significantly. Method LapPush was implemented in six clinics of the Kingdom of Swaziland. SMS results were sent out from the laboratory as a supplement to normal transport of paper results. Each clinic was equipped with a mobile phone to receive SMS results. The laboratory that processes the blood tests was equipped with a system for digital input of results, and transmission of results via SMS to the clinics. Results Laboratory results were received for 1041 different clinical cases. The total number of SMS records received (1032) was higher than that of paper records (965), indicating a higher loss rate for paper records. A statistical comparison of TAT for SMS and paper reports indicates a statistically significant improvement for SMS. Results were more positive for more rural clinics, and an urban clinic with high workload. Conclusion SMS can be used to reduce TAT for blood tests taken at clinics in developing countries. Benefits are likely to be greater at clinics that are further away from laboratories, due to the difficulties this imposes on transport of paper records. PMID:23028543

  20. Evaluation of major feed resources in crop-livestock mixed farming systems, southern Ethiopia: Indigenous knowledge versus laboratory analysis results

    Directory of Open Access Journals (Sweden)

    Deribe Gemiyo Talore

    2015-10-01

    Full Text Available Evaluation of major feed resources was conducted in four crop-livestock mixed farming systems of central southern Ethiopia, with 90 farmers, selected using multi-stage purposive and random sampling methods. Discussions were held with focused groups and key informants for vernacular name identification of feed, followed by feed sampling to analyse chemical composition (CP, ADF and NDF, in-vitro dry matter digestibility (IVDMD, and correlate with indigenous technical knowledge (ITK. Native pastures, crop residues (CR and multi-purpose trees (MPT are the major feed resources, demonstrated great variations in seasonality, chemical composition and IVDMD. The average CP, NDF and IVDMD values for grasses were 83.8 (ranged: 62.9–190, 619 (ranged: 357–877 and 572 (ranged: 317–743 g kg^(−1 DM, respectively. Likewise, the average CP, NDF and IVDMD for CR were 58 (ranged: 20–90, 760 (ranged: 340–931 and 461 (ranged: 285–637g kg^(−1 DM, respectively. Generally, the MPT and non-conventional feeds (NCF, Ensete ventricosum and Ipomoea batatas possessed higher CP (ranged: 155–164 g kg^(−1 DM and IVDMD values (611–657 g kg^(−1 DM while lower NDF (331–387 g kg^(−1 DM and ADF (321–344 g kg^(−1 DM values. The MPT and NCF were ranked as the best nutritious feeds by ITK while crop residues were the least. This study indicates that there are remarkable variations within and among forage resources in terms of chemical composition. There were also complementarities between ITK and feed laboratory results, and thus the ITK need to be taken into consideration in evaluation of local feed resources.

  1. Oxygen and chlorine isotopic fractionation during perchlorate biodegradation: Laboratory results and implications for forensics and natural attenuation studies

    Science.gov (United States)

    Sturchio, N.C.; Böhlke, J.K.; Beloso, A.D.; Streger, S.H.; Heraty, L.J.; Hatzinger, P.B.

    2007-01-01

    Perchlorate is a widespread environmental contaminant having both anthropogenic and natural sources. Stable isotope ratios of O and Cl in a given sample of perchlorate may be used to distinguish its source(s). Isotopic ratios may also be useful for identifying the extent of biodegradation of perchlorate, which is critical for assessing natural attenuation of this contaminant in groundwater. For this approach to be useful, however, the kinetic isotopic fractionations of O and Cl during perchlorate biodegradation must first be determined as a function of environmental variables such as temperature and bacterial species. A laboratory study was performed in which the O and Cl isotope ratios of perchlorate were monitored as a function of degradation by two separate bacterial strains (Azospira suillum JPLRND and Dechlorospirillum sp. FBR2) at both 10??C and 22??C with acetate as the electron donor. Perchlorate was completely reduced by both strains within 280 h at 22??C and 615 h at 10??C. Measured values of isotopic fractionation factors were ??18O = -36.6 to -29.0??? and ??37Cl = -14.5 to -11.5???, and these showed no apparent systematic variation with either temperature or bacterial strain. An experiment using 18O-enriched water (??18O = +198???) gave results indistinguishable from those observed in the isotopically normal water (??18O = -8.1???) used in the other experiments, indicating negligible isotope exchange between perchlorate and water during biodegradation. The fractionation factor ratio ??18O/??37Cl was nearly invariant in all experiments at 2.50 ?? 0.04. These data indicate that isotope ratio analysis will be useful for documenting perchlorate biodegradation in soils and groundwater. The establishment of a microbial fractionation factor ratio (??18O/??37Cl) also has significant implications for forensic studies. ?? 2007 American Chemical Society.

  2. Ectomycorrhizal inoculum potential of northeastern US forest soils for American chestnut restoration: results from field and laboratory bioassays.

    Science.gov (United States)

    Dulmer, Kristopher M; Leduc, Stephen D; Horton, Thomas R

    2014-01-01

    American chestnut (Castanea dentata) was once a dominant overstory tree in eastern USA but was decimated by chestnut blight (Cryphonectria parasitica). Blight-resistant chestnut is being developed as part of a concerted restoration effort to bring this heritage tree back. Here, we evaluate the potential of field soils in the northern portion of the chestnut's former range to provide ectomycorrhizal (EM) fungus inoculum for American chestnut. In our first study, chestnut seedlings were grown in a growth chamber using soil collected from three sites dominated by red oak (Quercus rubra) as inoculum and harvested after 5 months. Of the 14 EM fungi recovered on these seedlings, four species dominated in soils from all three sites: Laccaria laccata, a Tuber sp., Cenococcum geophilum, and a thelephoroid type. Seedlings grown in the nonsterilized soils were smaller than those growing in sterilized soils. In the second study, chestnut seedlings were grown from seed planted directly into soils at the same three sites. Seedlings with intermingling roots of established trees of various species were harvested after 5 months. Seventy-one EM fungi were found on the root tips of the hosts, with 38 occurring on chestnut seedlings. Multiple versus single host EM fungi were significantly more abundant and frequently encountered. The fungi observed dominating on seedlings in the laboratory bioassay were not frequently encountered in the field bioassay, suggesting that they may not have been active in mycelial networks in the field setting but were in the soils as resistant propagules that became active in the bioassay. These results show that soil from red oak stands can be used to inoculate American chestnut with locally adapted ectomycorrhizal fungi prior to outplanting, a relatively cost effective approach for restoration efforts.

  3. Memetics clarification of abnormal behavior

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: Biological medicine is hard to fully and scientifically explain the etiological factor and pathogenesis of abnormal behaviors; while, researches on philosophy and psychology (including memetics) are beneficial to better understand and explain etiological factor and pathogenesis of abnormal behaviors. At present, the theory of philosophy and psychology is to investigate the entity of abnormal behavior based on the views of memetics.METHODS: Abnormal behavior was researched in this study based on three aspects, including instinctive behavior disorder, poorly social-adapted behavior disorder and mental or body disease associated behavior disorder. Most main viewpoints of memetics were derived from "The Meme Machine", which was written by Susan Blackmore. When questions about abnormal behaviors induced by mental and psychological diseases and conduct disorder of teenagers were discussed, some researching achievements which were summarized by authors previously were added in this study, such as aggressive behaviors, pathologically aggressive behaviors, etc.RESULTS: The abnormal behaviors mainly referred to a part of people's substandard behaviors which were not according with the realistic social environment, culture background and the pathologic behaviors resulted from people's various psychological diseases. According to the theory of "meme", it demonstrated that the relevant behavioral obstacles of various psychological diseases, for example, the unusual behavior of schizophrenia, were caused, because the old meme was destroyed thoroughly but the new meme was unable to establish; psychoneurosis and personality disorder were resulted in hard establishment of meme; the behavioral obstacles which were ill-adapted to society, for example, various additional and homosexual behaviors, were because of the selfish replications and imitations of "additional meme" and "homosexual meme"; various instinct behavioral and congenital intelligent obstacles were not significance

  4. Alkaline phosphatase activity: new assay for the Reflotron system. Results of the evaluation in eight clinical laboratories.

    Science.gov (United States)

    Schumann, G; Dominick, H C; Hellmann, D; Klauke, R; Möckesch, M; Stekel, H; von Schenck, H; Kraft, M; Nagel, R; Hänseler, E

    2001-01-01

    A new reagent carrier, Reflotron ALP, has been developed for the Reflotron system, allowing easy and rapid measurement (in less than 3 minutes) of alkaline phosphatase (ALP) activity in capillary blood, venous blood, heparinized plasma or serum. The evaluation of the analytical performance of the assay was carried out at eight clinical laboratories. The study of the imprecision using the measurements in human samples resulted in coefficients of variation ranging from 1.3% to 4.6% (within-run) and from 3.2% to 4.0% (day-to-day). The analytical specificity of the Reflotron ALP assay agrees well with ALP methods using a N-methyl-D-glucamine buffer solution. The calibration of the Reflotron ALP assay, however, is related to the reference intervals for ALP methods using a diethanolamine buffer solution. Method comparisons were performed with the ALP method on Hitachi instruments using diethanolamine buffer. Reflotron ALP measurements in blood and plasma in 157 randomly selected split samples showed excellent agreement (slope: 0.99; intercept: 0.7 U/l; median bias: 2.3%; median difference from the comparison method: -0.3%). Specimens from pregnant women and adolescents were excluded from this study. Differing values were obtained in a method comparison using 48 samples containing predominantly the ALP bone isoform (slope: 0.81; intercept: 31.5 U/l; median bias: 5.7%; median difference from the comparison method: -12.2%). Regression analysis of the results from 21 sera with prevailing placental ALP gave a slope of 1.51, and an intercept of -41.1 U/l (median bias: 8.6%; median difference from the comparison method: 35.6%). Reflotron ALP was compared with three different wet chemistry procedures using different buffer compounds: N-methyl-D-glucamine or diethanolamine or 2-amino-2-methyl-1-propanol. In samples containing predominantly ALP isoforms not of liver origin, the measurements with N-methyl-D-glucamine buffer gave the best fit with respect to Reflotron. In an

  5. A Rare Stapes Abnormality

    Directory of Open Access Journals (Sweden)

    Hala Kanona

    2015-01-01

    Full Text Available The aim of this study is to increase awareness of rare presentations, diagnostic difficulties alongside management of conductive hearing loss and ossicular abnormalities. We report the case of a 13-year-old female reporting progressive left-sided hearing loss and high resolution computed tomography was initially reported as normal. Exploratory tympanotomy revealed an absent stapedius tendon and lack of connection between the stapes superstructure and footplate. The footplate was fixed. Stapedotomy and stapes prosthesis insertion resulted in closure of the air-bone gap by 50 dB. A review of world literature was performed using MedLine. Middle ear ossicular discontinuity can result in significant conductive hearing loss. This can be managed effectively with surgery to help restore hearing. However, some patients may not be suitable or decline surgical intervention and can be managed safely conservatively.

  6. Visualization Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Evaluates and improves the operational effectiveness of existing and emerging electronic warfare systems. By analyzing and visualizing simulation results...

  7. The DOSIS -Experiment onboard the Columbus Laboratory of the International Space Station -First Mission Results from the Active DOSTEL Instruments

    Science.gov (United States)

    Burmeister, Soenke; Berger, Thomas; Beaujean, Rudolf; Boehme, Matthias; Haumann, Lutz; Kortmann, Onno; Labrenz, Johannes; Reitz, Guenther

    Besides the effects of the microgravity environment, and the psychological and psychosocial problems encountered in confined spaces, radiation is the main health detriment for long dura-tion human space missions. The radiation environment encountered in space differs in nature from that on earth, consisting mostly of high energetic ions from protons up to iron, resulting in radiation levels far exceeding the ones encountered on earth for occupational radiation workers. Accurate knowledge of the physical characteristics of the space radiation field in dependence on the solar activity, the orbital parameters and the different shielding configurations of the International Space Station ISS is therefore needed. For the investigation of the spatial and temporal distribution of the radiation field inside the European COLUMBUS module the DLR experiment DOSIS (Dose Distribution Inside the ISS) was launched on July 15th 2009 with STS-127 to the ISS. The experimental package was transferred from the Space Shuttle into COLUMBUS on July 18th. It consists in a first part of a combination of passive detector packages (PDP) distributed at 11 locations inside the European Columbus Laboratory. The second part are two active radiation detectors (DOSTELs) with a DDPU (DOSIS Data and Power Unit) in a nomex pouch (DOSIS MAIN BOX) mounted at a fixed location beneath the European Physiology Module (EPM) inside COLUMBUS. After the successful installation the active part has been activated on the 18th July 2009. Each of the DOSTEL units consists of two 6.93 cm PIPS silicon detectors forming a telescope with an opening angle of 120. The two DOSTELs are mounted with their telescope axis perpendicular to each other to investigate anisotropies of the radiation field inside the COLUMBUS module especially during the passes through the South Atlantic Anomaly (SAA) and during Solar Particle Events (SPEs). The data from the DOSTEL units are transferred to ground via the EPM rack which is activated

  8. Use of a United States-based laboratory as a hematopathology reference center for a developing country: logistics and results.

    Science.gov (United States)

    Deetz, C O; Scott, M G; Ladenson, J H; Seyoum, M; Hassan, A; Kreisel, F H; Nguyen, T T; Frater, J L

    2013-02-01

    With proper logistical support and sponsorship, a laboratory in an industrialized nation might be able to act as a reference laboratory for clinicians based in a developing country. We built on previous experience in the clinical laboratory to see whether a specialized histopathology service (hematopathology) could be provided to a developing country without the expertise or experience to do it in country. Over an 13-year period, 582 cases from 579 individuals were analyzed. Principal pathologic findings included acute leukemia in 84 cases (14%), dyspoiesis in one or more of the hematopoietic lineages in 65 cases (11%, including three cases with high-grade myelodysplasia), 23 cases (4%) with findings suspicious for a chronic myeloproliferative disorder, 35 cases (6%) with findings suspicious for a lymphoproliferative disorder, and infectious organisms (presumably Leishmania in most instances) in 9 (1%) of cases. Specimens from 45 cases (8%) were unsatisfactory owing to extreme hemodilution and/or specimen degeneration. With proper support, a medical laboratory in an industrialized nation may serve as a reference facility for a developing nation. The use of existing infrastructure may be remarkably effective to achieve optimal turnaround time. Although the lack of ancillary studies and follow-up biopsies limit the ability to achieve a definitive diagnosis in many cases, this must be viewed in the context of the limited ability to diagnose or manage hematopoietic neoplasia in developing nations. © 2012 Blackwell Publishing Ltd.

  9. Evolutionary loss of the rdar morphotype in Salmonella as a result of high mutation rates during laboratory passage.

    Science.gov (United States)

    Davidson, Carla J; White, Aaron P; Surette, Michael G

    2008-03-01

    Rapid evolution of microbes under laboratory conditions can lead to domestication of environmental or clinical strains. In this work, we show that domestication due to laboratory passage in rich medium is extremely rapid. Passaging of wild-type Salmonella in rich medium led to diversification of genotypes contributing to the loss of a spatial phenotype, called the rdar morphotype, within days. Gene expression analysis of the rdar regulatory network demonstrated that mutations were primarily within rpoS, indicating that the selection pressure for scavenging during stationary phase had the secondary effect of impairing this highly conserved phenotype. If stationary phase was omitted from the experiment, radiation of genotypes and loss of the rdar morphotype was also demonstrated, but due to mutations within the cellulose biosynthesis pathway and also in an unknown upstream regulator. Thus regardless of the selection pressure, rapid regulatory changes can be observed on laboratory timescales. The speed of accumulation of rpoS mutations during daily passaging could not be explained by measured fitness and mutation rates. A model of mutation accumulation suggests that to generate the observed accumulation of sigma 38 mutations, this locus must experience a mutation rate of approximately 10(-4) mutations/gene/generation. Sequencing and gene expression of population isolates indicated that there were a wide variety of sigma 38 phenotypes within each population. This suggests that the rpoS locus is highly mutable by an unknown pathway, and that these mutations accumulate rapidly under common laboratory conditions.

  10. Improved accuracy and robustness of NAA results in a large throughput laboratory by systematic evaluation of internal quality control data

    NARCIS (Netherlands)

    Koster-Ammerlaan, M.J.J.; Bode, P.

    2009-01-01

    The laboratory for INAA in Delft processes several thousands of samples per year for multielement determinations in a variety of matrices. Samples are measured on different spectrometers (using well-type and coaxial detectors), by different persons and using many different analytical protocols. All

  11. Results of the Interlaboratory Exercise CNS/CIEMAT-04 Among Environmental Radioactivity Laboratories (Aqueous Solution); Resultados del Ejercicio Interlaboratorios de Radiactividad Ambiental CSN/CIEMAT-04 (Solucion Acuosa)

    Energy Technology Data Exchange (ETDEWEB)

    Romero Gonzalez, M. L.; Barrera Izquierdo, M.

    2004-07-01

    The document describes the outcome of the CSN/CIEMAT-04 interlaboratory test comparison among environmental radioactivity laboratories. The exercise was organised according to the ISO-43 and the ISO/IUPAC/AOAC Harmonised Protocol for the proficiency testing of analytical laboratories. Following the issue of the European Community Drinking Water Directive 98/83/EC concerning the quality of water for human consumption, the last inter-comparison exercise was organised by using a water sample, in an attempt to evaluate the performance of the laboratories analysing the required radioactivity parameters (H-3, gross alpha and beta activity and residual beta). The sample (a synthetic drinking water), was prepared at the National Laboratory for Ionising Radiation's Standards (CIEMAT), and contained the following radionuclides ''241 Am, ''239+240 Pu, ''90Sr, ''137 Cs, ''3 H y ''40 K. The results of the exercise were computed for 38 participating laboratories, and their analytical performance was assessed using the z-score approach. Robust statistics of the participant's results was applied to obtain the median and standard deviation, including suspected outliers. The exercise has revealed and homogeneous behaviour of laboratories, being statistical parameters from the results close to the reference values. A raised percentage os satisfactory laboratory performance has been obtained for gross alpha, gross beta and residual beta: 85, 97 and 87% respectively. The study has shown that participant laboratories perform radioactive determinations in drinking water samples with satisfactory quality levels. (Author) 16 refs.

  12. [Heterogenous abnormality polymorphism of gene PDGFRB in myeloid neoplasms and its clinical characteristics].

    Science.gov (United States)

    Wang, Quan-Shun; Gao, Li; Jing, Yu; Zhu, Hai-Yan; Yang, Hua; Yu, Li

    2012-04-01

    Myeloid neoplasms with eosinophilia and abnormalities of PDGFRB gene are a new kind of myeloid disorders in the revised 2008 WHO classification. Out of detected 2000 cases of myeloid cell abnormalities in our hospital, 12 cases of myeloid neoplasms with eosinophilia and abnormalities of PDGFRB were found. This study was purposed to summarize and analyze the clinical and laboratorial characteristics of the 12 cases with PDGFRB gene abnormalities. The results indicated that among 12 cases of myeloid neoplasms with PDGFRB abnormalities, 5 cases with TEL/PDGFRB fusion gene, 2 cases with HEPI/PDGFRB, 1 case with PDGFRB mutation, 1 case with RABAPTIN-5/PDGFRB, 1 case with GIT2/PDGFRB, 1 case with TP53/PDGFRB, 1 case with WDR43/PDGFRB fusion gene were detected, showing the polymorphism of PDGFRB gene abnormalities. Among this kind of myeloid neoplasm patients, almost all patients manifested monocytosis and eosinophilia in different degree, the thrombocytosis mainly was observed in atypical myeloid neoplasms, acute leukemia, chromic myelo-monocytic leukemia patients. The treatment with imatinib mesylate for this kind of patients was effective in some cases. It is concluded that the myeloid neoplasms with PDGFRB gene abnormalities are a kind of heterogenetic myeloid neoplasms, their gene abnormal types and clinical manifestations show polymorphism too. The monocytosis and eosinophilia appear in this kind myeloid neoplasms which may be treated with tyrosine kinase inhibitors such as imatinib mesylate.

  13. SERUM CHEMISTRY ABNORMALITIES IN CHILDREN WITH UNPROVOKED SEIZURES

    Directory of Open Access Journals (Sweden)

    J. Akhondian MD

    2009-01-01

    Full Text Available ObjectiveMost children brought to the emergency department (ED for evaluation of seizures undergo an extensive laboratory workup. Since results are usually negative, the value of such routine laboratory workups has been questioned. A group of children with unprovoked seizures was prospectively studied to determine the diagnostic values of routine serum chemistries and to identify risk factors predictive of abnormal findings.Materials & MethodsAll patients evaluated at the ED of the Ghaem hospital during a consecutive 12 months period between Jan 2004 through Jan 2005 were studied. We collected data for patient's demographics, details of the history of present illness (including vomiting, diarrhea, apnea, medication use, past history of seizures, family history of seizures, metabolic disorders or other chronic medical illnesses, neonatal history and neurological examination as well as nutritional status, type and time of seizure. The role of abnormal serum chemistries as a seizure trigger factor was assessed in patients with a history of seizure.ResultsA total of 210 patients (mean age 19.2 months with unprovoked seizures were evaluated. Twenty- three serum abnormalities were noted in the patients (12 cases of hyponatremia, 7 of hypoglycemia, 4 of hypokalemia, 4 of uremia. The incidence of abnormal serum biochemical values was higher in patients with a first seizure, younger patients, and those with gastrointestinal symptoms.ConclusionAccording to the present study, one can conclude that in children younger than 2 years and having no structural CNS abnormality, electrolyte and glucose screening is recommended only for a first unprovoked seizure, when gastrointestinal symptoms or symptoms suggesting electrolyte disturbances are present.Keywords:Unprovoked, Seizure, Biochemistry, Children

  14. SERUM CHEMISTRY ABNORMALITIES IN CHILDREN WITH UNPROVOKED SEIZURES

    Directory of Open Access Journals (Sweden)

    J. Akhondian MD,

    2007-02-01

    Full Text Available ObjectiveMost children brought to the emergency department (ED for evaluation of seizures undergo an extensive laboratory workup. Since results are usually negative, the value of such routine laboratory workups has been questioned. A group of children with unprovoked seizures was prospectively studied to determine the diagnostic values of routine serum chemistries and to identify risk factors predictive of abnormal findings.Materials & MethodsAll patients evaluated at the ED of the Ghaem hospital during a consecutive 12 months period between Jan 2004 through Jan 2005 were studied. We collected data for patient's demographics, details of the history of present illness (including vomiting, diarrhea, apnea, medication use, past history of seizures, family history of seizures, metabolic disorders or other chronic medical illnesses, neonatal history and neurological examination as well as nutritional status, type and time of seizure. The role of abnormal serum chemistries as a seizure trigger factor was assessed in patients with a history of seizure.ResultsA total of 210 patients (mean age 19.2 months with unprovoked seizures were evaluated. Twenty- three serum abnormalities were noted in the patients (12 cases of hyponatremia, 7 of hypoglycemia, 4 of hypokalemia, 4 of uremia. The incidence of abnormal serum biochemical values was higher in patients with a first seizure, younger patients, and those with gastrointestinal symptoms.ConclusionAccording to the present study, one can conclude that in children younger than 2 years and having no structural CNS abnormality, electrolyte and glucose screening is recommended only for a first unprovoked seizure, when gastrointestinal symptoms or symptoms suggesting electrolyte disturbances are present.

  15. Brine formation via deliquescence by salts found near Don Juan Pond, Antarctica: Laboratory experiments and field observational results

    Science.gov (United States)

    Gough, R. V.; Wong, J.; Dickson, J. L.; Levy, J. S.; Head, J. W.; Marchant, D. R.; Tolbert, M. A.

    2017-10-01

    The observed darkening of water tracks near Don Juan Pond (DJP) as well as the formation of wet patches elsewhere in the McMurdo Dry Valleys is attributed at least partially to deliquescence, a process by which salts absorb atmospheric water vapor and form brine, coupled with liquid-phase growth when the atmospheric relative humidity exceeds the water activity. Here we perform laboratory experiments to investigate the temperature and relative humidity conditions necessary for deliquescence to occur in calcium chloride-rich sediments collected from the DJP watershed. We use a Raman microscope equipped with an environmental cell to study both deliquescence and efflorescence (recrystallization) of the soluble salt component of DJP soils between -30 and +15 °C. In this temperature range, we find that the soluble salt component of the DJP sediments begins to deliquesce between 19 and 46% RH, slightly higher than the deliquescence relative humidity of the primary pure component, calcium chloride. We find a limited hysteresis between deliquescence and efflorescence, but much greater supersaturation of the salt brine can occur at temperatures above 0 °C. The relative humidity conditions were varied either slowly (over ∼8 h) to observe near-equilibrium phases or rapidly (over work can help predict when deliquescence could be actively occurring in the soils near Don Juan Pond and explain darkening of the salt pan after a high humidity period. In tandem with field data, our experimental results suggest that brines can be generated near Don Juan Pond via deliquescence frequently during the southern summer and autumn. Additionally, the soluble salts may persist in the aqueous phase continuously for several months during the southern summer. This work also suggests that salt deliquescence could be impacting the year-round hydrological cycle of the DJP watershed. Steep-sloped water tracks found near DJP have been suggested as a terrestrial analog for recurring slope lineae on

  16. Boron deficiency results in early repression of a cytokinin receptor gene and abnormal cell differentiation in the apical root meristem of Arabidopsis thaliana.

    Science.gov (United States)

    Abreu, Isidro; Poza, Laura; Bonilla, Ildefonso; Bolaños, Luis

    2014-04-01

    The development of Arabidopsis thaliana was dramatically altered within few hours following boron (B) deprivation. This effect was particularly evident in the apical root meristem. The essentiality of boron in plants has been clearly linked to its structural role in the cell wall, however the diversity and rapidity alterations of plant organogenesis when the micronutrient is absent suggest that B deficiency could also affect gene regulation during plant development. Therefore, the effect of B deficiency on cell elongation, apical root meristem cell division, and early differentiation of root tissues was investigated in A. thaliana seedlings. Dark-growth experiments indicated that hypocotyl elongation was inhibited 2 days after removing B, but apical root growth ceased almost immediately following B deprivation. Detection of cycline B1 by GUS staining of a promoter-reporter construct revealed that low B led to a reduced zone of cell division. The expression of CRE1/WOL/AHK4, encoding an integral membrane protein with histidine kinase domain that mediates cytokinin signaling and root xylem differentiation, was inhibited under B deficiency resulting in arrested xylem development at the protoxylem stage. Because the transition from cell division to cell differentiation in apical root meristems is controlled by cytokinins, this result support the hypothesis that signaling mechanisms during cell differentiation and organogenesis are highly sensitive to B deficiency, and together with previous reports that link the micronutrient with auxin or ethylene control of root architecture, suggests that B could play a role in regulation of hormone mediated early plant development signaling. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  17. Using a reference when defining an abnormal MRI reduces false-positive MRI results-a longitudinal study in two cohorts at risk for rheumatoid arthritis.

    Science.gov (United States)

    Boer, Aleid C; Burgers, Leonie E; Mangnus, Lukas; Ten Brinck, Robin M; Nieuwenhuis, Wouter P; van Steenbergen, Hanna W; Reijnierse, Monique; Huizinga, Tom W J; van der Helm van Mil, Annette H M

    2017-10-01

    The use of hand and foot MRI in the diagnostic process of RA has been advocated. Recent studies showed that MRI is helpful in predicting progression from clinically suspect arthralgia (CSA) to clinical arthritis, and from undifferentiated arthritis (UA) to RA. Symptom-free persons can also show inflammation on MRI. This study aimed to evaluate if MRI findings in symptom-free volunteers are relevant when defining a positive MRI. Two hundred and twenty-five CSA patients and two hundred and one UA patients underwent MRI of MCP, wrist and MTP joints at baseline and were followed for 1 year on progression to arthritis and RA, respectively, as reported previously. MRI was considered positive if ⩾ 1 joint showed inflammation (called uncorrected definition), or if ⩾ 1 joint had inflammation that was present in < 5% of persons of the same age category at the same location (called 5% corrected definition). Test characteristics were compared for both definitions. By using MRI data of symptom-free volunteers as reference, specificity of MRI-detected inflammation increased from 22 to 56% in CSA patients, and from 10 to 36% in UA patients. The sensitivity was not affected; it was 88 and 85% in CSA patients and 93 and 93% in UA patients. The accuracy also increased, from 32 to 60% in CSA patients and 22 to 44% in UA patients. The use of a reference population resulted in a substantial reduction of false-positive results, without influencing the sensitivity. Although common for other tests in medicine, this phenomenon is novel for MRI in the early detection of RA.

  18. Hydrogeological characterization based on the results of long term monitoring in the Mizunami Underground Research Laboratory Project

    Science.gov (United States)

    Takeuchi, R.; Ohyama, T.; Matsuoka, T.; Saegusa, H.; Takeuchi, S.

    2009-12-01

    The Mizunami Underground Research Laboratory (MIU) is now under construction by the Japan Atomic Energy Agency in the Cretaceous Toki granite in the Tono area of central Japan. Conceptual design of the MIU consists of two 1,000 m shafts (the Main Shaft and the Ventilation Shaft) and horizontal research galleries. The MIU project is a broad scientific study of the deep geological environment as a base for the research and development for geological disposal of nuclear wastes. One of the main goals is to establish comprehensive techniques for investigation, analysis and assessment of the deep geological environment in fractured crystalline rock. The project is being implemented in three overlapping phases: Surface-based Investigation (Phase I), Construction (Phase II) and Operation (Phase III), with a total duration of 20 years. In Phase I, surface investigations were carried out in a stepwise manner in order to obtain information of the geological environment at the site scale (about 2km square). Geological modeling and simulations of various kinds had been carried out in order to synthesize these investigation results. Two NNW-trending faults, which are important for hydrogeological characterization, are included in the model. One of the faults (fault A) strikes through the site in the immediate vicinity of the Main Shaft and another fault (fault B) strikes through the southern part of the MIU construction site. In Phase II, field investigations have been carried out in and around the MIU construction site. For hydrogeological characterization, long term monitoring of hydraulic pressure, surface tilt and self-potential have been carried out on surface and in the research galleries to obtain information on changes of groundwater flow due to shaft excavation. The results of the long term monitoring focused on fault A are as follows: - The hydraulic pressure responses are observed in surface boreholes in and around the MIU construction site and the galleries. The

  19. Deficits in Col5a2 Expression Result in Novel Skin and Adipose Abnormalities and Predisposition to Aortic Aneurysms and Dissections.

    Science.gov (United States)

    Park, Arick C; Phan, Noel; Massoudi, Dawiyat; Liu, Zhenjie; Kernien, John F; Adams, Sheila M; Davidson, Jeffrey M; Birk, David E; Liu, Bo; Greenspan, Daniel S

    2017-10-01

    Classic Ehlers-Danlos syndrome (cEDS) is characterized by fragile, hyperextensible skin and hypermobile joints. cEDS can be caused by heterozygosity for missense mutations in genes COL5A2 and COL5A1, which encode the α2(V) and α1(V) chains, respectively, of collagen V, and is most often caused by COL5A1 null alleles. However, COL5A2 null alleles have yet to be associated with cEDS or other human pathologies. We previously showed that mice homozygous null for the α2(V) gene Col5a2 are early embryonic lethal, whereas haploinsufficiency caused aberrancies of adult skin, but not a frank cEDS-like phenotype, as skin hyperextensibility at low strain and dermal cauliflower-contoured collagen fibril aggregates, two cEDS hallmarks, were absent. Herein, we show that ubiquitous postnatal Col5a2 knockdown results in pathognomonic dermal cauliflower-contoured collagen fibril aggregates, but absence of skin hyperextensibility, demonstrating these cEDS hallmarks to arise separately from loss of collagen V roles in control of collagen fibril growth and nucleation events, respectively. Col5a2 knockdown also led to loss of dermal white adipose tissue (WAT) and markedly decreased abdominal WAT that was characterized by miniadipocytes and increased collagen deposition, suggesting α2(V) to be important to WAT development/maintenance. More important, Col5a2 haploinsufficiency markedly increased the incidence and severity of abdominal aortic aneurysms, and caused aortic arch ruptures and dissections, indicating that α2(V) chain deficits may play roles in these pathologies in humans. Copyright © 2017 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

  20. CHROMOSOME ABNORMALITIES IN INFERTILITY

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    Mateja Smogavec

    2009-08-01

    Conclusions Chromosomal analysis is an important method in diagnostic procedures of infertility, because chromosomal abnormalities could play the important role in etiology of infertility and are more frequently detected in this group of patients compared to general population. In the infertile couples balanced chromosomal abnormalities are the main cause of spontaneous abortions. Sex chromosome aneuploidies are highly correlated to infertility of females and males.

  1. Hereditary urea cycle abnormality

    Science.gov (United States)

    ... vitro so the specific genetic cause is known. Teamwork between parents, the affected child, and doctors can help prevent severe illness. Alternative Names Abnormality of the urea cycle - hereditary; Urea cycle - hereditary abnormality Images Male urinary system Urea cycle References Lichter-Konecki ...

  2. Analysis of bias in measurements of potassium, sodium and hemoglobin by an emergency department-based blood gas analyzer relative to hospital laboratory autoanalyzer results.

    Directory of Open Access Journals (Sweden)

    Jian Bo Zhang

    Full Text Available The emergency departments (EDs of Chinese hospitals are gradually being equipped with blood gas machines. These machines, along with the measurement of biochemical markers by the hospital laboratory, facilitate the care of patients with severe conditions who present to the ED. However, discrepancies have been noted between the Arterial Blood Gas (ABG analyzers in the ED and the hospital laboratory autoanalyzer in relation to electrolyte and hemoglobin measurements. The present study was performed to determine whether the ABG and laboratory measurements of potassium, sodium, and hemoglobin levels are equivalent, and whether ABG analyzer results can be used to guide clinical care before the laboratory results become available.Study power analyses revealed that 200 consecutive patients who presented to our ED would allow this prospective single-center cohort study to detect significant differences between ABG- and laboratory-measured potassium, sodium, and hemoglobin levels. Paired arterial and venous blood samples were collected within 30 minutes. Arterial blood samples were measured in the ED by an ABL 90 FLEX blood gas analyzer. The biochemistry and blood cell counts of the venous samples were measured in the hospital laboratory. The potassium, sodium, and hemoglobin concentrations obtained by both methods were compared by using paired Student's t-test, Spearman's correlation, Bland-Altman plots, and Deming regression.The mean ABG and laboratory potassium values were 3.77±0.44 and 4.2±0.55, respectively (P<0.0001. The mean ABG and laboratory sodium values were 137.89±5.44 and 140.93±5.50, respectively (P<0.0001. The mean ABG and laboratory Hemoglobin values were 12.28±2.62 and 12.35±2.60, respectively (P = 0.24.Although there are the statistical difference and acceptable biases between ABG- and laboratory-measured potassium and sodium, the biases do not exceed USCLIA-determined limits. In parallel, there are no statistical differences and

  3. Analysis of bias in measurements of potassium, sodium and hemoglobin by an emergency department-based blood gas analyzer relative to hospital laboratory autoanalyzer results.

    Science.gov (United States)

    Zhang, Jian Bo; Lin, Ji; Zhao, Xiao Dong

    2015-01-01

    The emergency departments (EDs) of Chinese hospitals are gradually being equipped with blood gas machines. These machines, along with the measurement of biochemical markers by the hospital laboratory, facilitate the care of patients with severe conditions who present to the ED. However, discrepancies have been noted between the Arterial Blood Gas (ABG) analyzers in the ED and the hospital laboratory autoanalyzer in relation to electrolyte and hemoglobin measurements. The present study was performed to determine whether the ABG and laboratory measurements of potassium, sodium, and hemoglobin levels are equivalent, and whether ABG analyzer results can be used to guide clinical care before the laboratory results become available. Study power analyses revealed that 200 consecutive patients who presented to our ED would allow this prospective single-center cohort study to detect significant differences between ABG- and laboratory-measured potassium, sodium, and hemoglobin levels. Paired arterial and venous blood samples were collected within 30 minutes. Arterial blood samples were measured in the ED by an ABL 90 FLEX blood gas analyzer. The biochemistry and blood cell counts of the venous samples were measured in the hospital laboratory. The potassium, sodium, and hemoglobin concentrations obtained by both methods were compared by using paired Student's t-test, Spearman's correlation, Bland-Altman plots, and Deming regression. The mean ABG and laboratory potassium values were 3.77±0.44 and 4.2±0.55, respectively (P<0.0001). The mean ABG and laboratory sodium values were 137.89±5.44 and 140.93±5.50, respectively (P<0.0001). The mean ABG and laboratory Hemoglobin values were 12.28±2.62 and 12.35±2.60, respectively (P = 0.24). Although there are the statistical difference and acceptable biases between ABG- and laboratory-measured potassium and sodium, the biases do not exceed USCLIA-determined limits. In parallel, there are no statistical differences and biases

  4. External Quality Assurance System (EQAS) of the WHO Global Salmonella Surveillance and Laboratory Support Project (Global Salm-Surv) Results from 2001

    DEFF Research Database (Denmark)

    Petersen, A.; Aarestrup, Frank Møller; Evans, M.C.

    An international collaborative study on serotyping and antimicrobial susceptibility testing of eight Salmonella enterica strains was performed to enhance the capacity of national and regional reference laboratories in WHO Global Salm-Surv to conduct Salmonella serotyping and antimicrobial suscept...... of the quality control range specified by NCCLS guidelines....... susceptibility testing. A total of 103 laboratories in 60 countries participated. For serotyping, 78% of the results were correct. For susceptibility testing, 91% of the results were in agreement with the expected results. However, 17% of the performed tests with the E. coli ATCC 25922 reference strain were out...

  5. Kidney transplantation in abnormal bladder

    Directory of Open Access Journals (Sweden)

    Shashi K Mishra

    2007-01-01

    Full Text Available Structural urologic abnormalities resulting in dysfunctional lower urinary tract leading to end stage renal disease may constitute 15% patients in the adult population and up to 20-30% in the pediatric population. A patient with an abnormal bladder, who is approaching end stage renal disease, needs careful evaluation of the lower urinary tract to plan the most satisfactory technical approach to the transplant procedure. Past experience of different authors can give an insight into the management and outcome of these patients. This review revisits the current literature available on transplantation in abnormal bladder and summarizes the clinical approach towards handling this group of difficult transplant patients. We add on our experience as we discuss the various issues. The outcome of renal transplant in abnormal bladder is not adversely affected when done in a reconstructed bladder. Correct preoperative evaluation, certain technical modification during transplant and postoperative care is mandatory to avoid complications. Knowledge of the abnormal bladder should allow successful transplantation with good outcome.

  6. Results of the Interlaboratory Exercise CSN/CIEMAT-100 Among Environmental Radioactivity Laboratories (Soil); Resultados del Ejercicio Interlaboratorios de Radiactividad Ambiental CSN/CIEMAT-00 (Suelo)

    Energy Technology Data Exchange (ETDEWEB)

    Romero Gonzalez, M. L.

    2002-07-01

    The document describes the outcome of the CSN/CIEMAT-00 interlaboratory test comparison among environmental radioactivity laboratories. the exercise was organised according to the ISO-43 and the ISO/IUPAC/AOAC Harmonized Protocol for the proficiency testing of analytical laboratories. the test sample was a soil containing environmental levels of K-40, Ra-226, Ac-228, Sr-90, Cs-137, Cs-134, Pu (239-240) y Am-241. the Universidad Autonoma de Barcelona prepared the material and reported adequate statistical studies of homogeneity. The results of the exercise were computed for 30 participating laboratories, and their analytical performance was assessed using the u-score approach. A raised percentage of satisfactory laboratory performance has been obtained for all the analysis, being the best performance in gamma measurements. The exercise has drawn that several laboratories have difficulties in the evaluation of combined uncertainty, mainly in analysis involving radiochemical steps. The study has shown an homogeneous inter-laboratory behaviour, and the improvement achieved through subsequent exercises in the quality of the data they are producing. (Author) 10 refs.

  7. Abnormal insulin levels and vertigo.

    Science.gov (United States)

    Proctor, C A

    1981-10-01

    Fifty patients with unexplained vertigo (36) or lightheadedness (14) are evaluated, all of whom had abnormal ENGs and normal audiograms. Five hour insulin glucose tolerance tests were performance on all patients, with insulin levels being obtained fasting and at one-half, one, two, and three hours. The results of this investigation were remarkable. Borderline or abnormal insulin levels were discovered in 82% of patients; 90% were found to have either an abnormal glucose tolerance test or at least borderline insulin levels. The response to treatment in these dizzy patients was also startling, with appropriate low carbohydrate diets improving the patient's symptoms in 90% of cases. It is, therefore, apparent that the earliest identification of carbohydrate imbalance with an insulin glucose tolerance test is extremely important in the work-up of the dizzy patients.

  8. Thyroid abnormality in perimenopausal women with abnormal uterine bleeding

    Directory of Open Access Journals (Sweden)

    Prasanna Byna

    2015-11-01

    Full Text Available Background: AUB is a common but complicated clinical presentation and occurs in 15-20% of women between menarche to menopause and significantly affects the women's health. Women with thyroid dysfunction often have menstrual irregularities, infertility and increased morbidity during pregnancy. The objective of present study is to find the correlation between thyroid disorders and AUB in perimenopausal women attending gynecology OPD. Methods: In the present study, fifty five patients with AUB were included and were evaluated for the cause including thyroid abnormality. Thyroid function tests were done in all patients. Results: Among 55 patients, 12 patients were diagnosed as hypothyroidism and 7 as hyperthyroidism, women with AUB 36 (65.4% were euthyroid. Among 19 women with thyroid abnormality, heavy menstrual bleeding was seen in 8 (42% women, 6 (31.57% had polymenorrhagia, 5 (26.31% had oligomenorrhoea. The frequent menstrual abnormality in women with hypothyroidism (12 women was heavy menstrual bleeding in 5 (41.6% women, 3 (25% had oligomennorhoea, 4 (33.3% had polymenorrhagia. Out of 7 women with hyperthyroidism, 2 (28.57% had oligomenorrhoea, 3 (42.8% had heavy menstrual bleeding, 2 (28.57% had polymenorrhagia. In a total of 55 patients with AUB, 11 (20% had structural abnormalities in uterus and ovaries. 5 (9% had adenomyosis, 3 (5.4% had ovarian cysts, 3 (5.4% had fibroids. Conclusions: It is important to screen all women for thyroid abnormality who are presenting with AUB especially with non-structural causes of AUB. Correction of thyroid abnormalities also relieves AUB. This will avoid unnecessary hormonal treatment and surgery. [Int J Res Med Sci 2015; 3(11.000: 3250-3253

  9. In Vitro Preclinical Testing of Nonoxynol-9 as Potential Anti-Human Immunodeficiency Virus Microbicide: a Retrospective Analysis of Results from Five Laboratories

    Science.gov (United States)

    Beer, Brigitte E.; Doncel, Gustavo F.; Krebs, Fred C.; Shattock, Robin J.; Fletcher, Patricia S.; Buckheit, Robert W.; Watson, Karen; Dezzutti, Charlene S.; Cummins, James E.; Bromley, Ena; Richardson-Harman, Nicola; Pallansch, Luke A.; Lackman-Smith, Carol; Osterling, Clay; Mankowski, Marie; Miller, Shendra R.; Catalone, Bradley J.; Welsh, Patricia A.; Howett, Mary K.; Wigdahl, Brian; Turpin, Jim A.; Reichelderfer, Patricia

    2006-01-01

    The first product to be clinically evaluated as a microbicide contained the nonionic surfactant nonoxynol-9 (nonylphenoxypolyethoxyethanol; N-9). Many laboratories have used N-9 as a control compound for microbicide assays. However, no published comparisons of the results among laboratories or attempts to establish standardized protocols for preclinical testing of microbicides have been performed. In this study, we compared results from 127 N-9 toxicity and 72 efficacy assays that were generated in five different laboratories over the last six years and were performed with 14 different cell lines or tissues. Intra-assay reproducibility was measured at two-, three-, and fivefold differences using standard deviations. Interassay reproducibility was assessed using general linear models, and interaction between variables was studied using step-wise regression. The intra-assay reproducibility within the same N-9 concentration, cell type, assay duration, and laboratory was consistent at the twofold level of standard deviations. For interassay reproducibility, cell line, duration of assay, and N-9 concentration were all significant sources of variability (P 24 h) and short (<2 h) exposures of cells to N-9 showed variability, while assays with 4 to 8 h of N-9 exposure gave results that were not significantly different. This is the first analysis to compare preclinical N-9 toxicity levels that were obtained by different laboratories using various protocols. This comparative work can be used to develop standardized microbicide testing protocols that will help advance potential microbicides to clinical trials. PMID:16436731

  10. SeqReporter: automating next-generation sequencing result interpretation and reporting workflow in a clinical laboratory.

    Science.gov (United States)

    Roy, Somak; Durso, Mary Beth; Wald, Abigail; Nikiforov, Yuri E; Nikiforova, Marina N

    2014-01-01

    A wide repertoire of bioinformatics applications exist for next-generation sequencing data analysis; however, certain requirements of the clinical molecular laboratory limit their use: i) comprehensive report generation, ii) compatibility with existing laboratory information systems and computer operating system, iii) knowledgebase development, iv) quality management, and v) data security. SeqReporter is a web-based application developed using ASP.NET framework version 4.0. The client-side was designed using HTML5, CSS3, and Javascript. The server-side processing (VB.NET) relied on interaction with a customized SQL server 2008 R2 database. Overall, 104 cases (1062 variant calls) were analyzed by SeqReporter. Each variant call was classified into one of five report levels: i) known clinical significance, ii) uncertain clinical significance, iii) pending pathologists' review, iv) synonymous and deep intronic, and v) platform and panel-specific sequence errors. SeqReporter correctly annotated and classified 99.9% (859 of 860) of sequence variants, including 68.7% synonymous single-nucleotide variants, 28.3% nonsynonymous single-nucleotide variants, 1.7% insertions, and 1.3% deletions. One variant of potential clinical significance was re-classified after pathologist review. Laboratory information system-compatible clinical reports were generated automatically. SeqReporter also facilitated quality management activities. SeqReporter is an example of a customized and well-designed informatics solution to optimize and automate the downstream analysis of clinical next-generation sequencing data. We propose it as a model that may envisage the development of a comprehensive clinical informatics solution. Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  11. Does a possible laboratory observation of a frequency anisotropy of light result from a non-zero photon mass m?

    Science.gov (United States)

    Narlikar, J. V.; Pecker, J. C.; Vigier, J. P.

    1991-04-01

    Assuming a priori the existence of a non-zero photon rest-mass mγ>0 and justifying this assumption, we can predict the existence of an anisotropy in velocity and frequency of light in the direction of the apex of the 3 K background cosmic radiation field. Since this frequency shift can now be tested in the laboratory, it is important to improve the precision of these measurements in order to check our predictions. Its possible confirmation implies indeed the definition of an absolute evolution parameter in the rest frame Σ0 of this 3 K background microwave radiation.

  12. Shaking of pyroclastic cones and the formation of granular flows on their flanks: Results from laboratory experiments

    Science.gov (United States)

    Cagnoli, B.; Romano, G. P.; Ventura, G.

    2015-11-01

    We have carried out laboratory experiments to study the generation of granular flows on the slopes of pyroclastic cones that are experiencing volcanic tremor or tectonic earthquakes. These experiments are inspired by the occurrence of granular flows on the flanks of Mount Vesuvius during its 1944 eruption. Our laboratory model consists of sand cones built around a vibrating tube which represents a volcanic conduit with erupting magma inside. A video camera allows the study of the granular flow inception, movement and deposition. Although the collapse of the entire cone is obtained at a specific resonance frequency, single granular flows can be generated by all the vibration frequencies (1-16 Hz) and all the vibration amplitudes (0.5-1.5 mm) that our experimental apparatus has allowed us to adopt. We believe that this is due to the fact that the energy threshold to trigger the flows is small in value. Therefore, if this is true in nature as well, shaken pyroclastic cones are always potentially dangerous because they can easily generate flows that can strike the surrounding areas.

  13. Abnormal menstrual periods (image)

    Science.gov (United States)

    ... may have a variety of causes, such as endometrial hyperplasia, endometrial polyps, uterine fibroids, and abnormal thyroid or ... the endometrium becomes unusually thick it is called endometrial ... Hyperplasia may cause profuse or extended menstrual bleeding.

  14. "Jeopardy" in Abnormal Psychology.

    Science.gov (United States)

    Keutzer, Carolin S.

    1993-01-01

    Describes the use of the board game, Jeopardy, in a college level abnormal psychology course. Finds increased student interaction and improved application of information. Reports generally favorable student evaluation of the technique. (CFR)

  15. Chromosomal Abnormalities in ADHD

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2002-07-01

    Full Text Available The prevalence of fragile X syndrome, velocardiofacial syndrome (VCFS, and other cytogenetic abnormalities among 100 children (64 boys with combined type ADHD and normal intelligence was assessed at the NIMH and Georgetown University Medical Center.

  16. Chromosomal abnormalities and autism

    Directory of Open Access Journals (Sweden)

    Farida El-Baz

    2016-01-01

    Conclusion: Chromosomal abnormalities were not detected in the studied autistic children, and so the relation between the genetics and autism still needs further work up with different study methods and techniques.

  17. First results on low-mass WIMP from the CDEX-1 experiment at the China Jinping underground Laboratory

    CERN Document Server

    Zhao, W; Kang, K J; Cheng, J P; Li, Y J; Lin, S T; Bai, Y; Bi, Y; Chang, J P; Chen, N; Chen, Q H; Chen, Y H; Chuang, Y C; Deng, Z; Du, C; Du, Q; Gong, H; Hao, X Q; He, H J; He, Q J; Hu, X H; Huang, H X; Huang, T R; Jiang, H; Li, H B; Li, J M; Li, J; Li, X; Li, X Y; Li, Y L; Liao, H Y; Lin, F K; Liu, S K; Lv, L C; Ma, H; Mao, S J; Qin, J Q; Ren, J; Ruan, X C; Shen, M B; Singh, L; Singh, M K; Soma, A K; Su, J; Tang, C J; Tseng, C H; Wang, J M; Wang, L; Wang, Q; Wong, H T; Wu, S Y; Wu, W; Wu, Y C; Xianyu, Z Z; Xing, H Y; Xu, Y; Xu, X J; Xue, T; Yang, L T; Yang, S W; Yi, N; Yu, C X; Yu, H; Yu, X Z; Zeng, X H; Zeng, Z; Zhang, L; Zhang, Y H; Zhao, M G; Zhong, S N; Zhou, Z Y; Zhu, J J; Zhu, W B; Zhu, X Z; Zhu, Z H

    2013-01-01

    The China Dark matter Experiment collaboration reports the first experimental limit on WIMP dark matter from 14.6 kg-day of data taken with a 994 g p-type point-contact germanium detector at the China Jinping underground Laboratory where the rock overburden is more than 2400 m. The energy threshold achieved was 400 eVee. According to the 14.6 kg-day live data, we placed the limit of N= 1.75 * 10^{-40} cm^{2} at 90% confidence level on the spin-independent cross-section at WIMP mass of 7 GeV before differentiating bulk signals from the surface backgrounds.

  18. First results on low-mass WIMPs from the CDEX-1 experiment at the China Jinping underground laboratory

    Science.gov (United States)

    Zhao, W.; Yue, Q.; Kang, K. J.; Cheng, J. P.; Li, Y. J.; Lin, S. T.; Bai, Y.; Bi, Y.; Chang, J. P.; Chen, N.; Chen, N.; Chen, Q. H.; Chen, Y. H.; Chuang, Y. C.; Deng, Z.; Du, C.; Du, Q.; Gong, H.; Hao, X. Q.; He, H. J.; He, Q. J.; Hu, X. H.; Huang, H. X.; Huang, T. R.; Jiang, H.; Li, H. B.; Li, J. M.; Li, J.; Li, J.; Li, X.; Li, X. Y.; Li, Y. L.; Liao, H. Y.; Lin, F. K.; Liu, S. K.; Lü, L. C.; Ma, H.; Mao, S. J.; Qin, J. Q.; Ren, J.; Ren, J.; Ruan, X. C.; Shen, M. B.; Singh, L.; Singh, M. K.; Soma, A. K.; Su, J.; Tang, C. J.; Tseng, C. H.; Wang, J. M.; Wang, L.; Wang, Q.; Wong, H. T.; Wu, S. Y.; Wu, W.; Wu, Y. C.; Wu, Y. C.; Xianyu, Z. Z.; Xing, H. Y.; Xu, Y.; Xu, X. J.; Xue, T.; Yang, L. T.; Yang, S. W.; Yi, N.; Yu, C. X.; Yu, H.; Yu, X. Z.; Zeng, X. H.; Zeng, Z.; Zhang, L.; Zhang, Y. H.; Zhao, M. G.; Zhong, S. N.; Zhou, Z. Y.; Zhu, J. J.; Zhu, W. B.; Zhu, X. Z.; Zhu, Z. H.

    2013-09-01

    The China Dark Matter Experiment Collaboration reports the first experimental limit on weakly interacting massive particles (WIMPs) dark matter from 14.6 kg-days of data taken with a 994 g p-type point-contact germanium detector at the China Jinping underground laboratory where the rock overburden is more than 2400 m. The energy threshold achieved was 400 eVee. According to the 14.6 kg-day live data, we placed the limit of σχN=1.75×10-40cm2 at a 90% confidence level on the spin-independent cross section at a WIMP mass of 7 GeV before differentiating bulk signals from the surface backgrounds.

  19. Recent experimental results of effects of perfluoroalkyl substances in laboratory animals - Relation to current regulations and guidance values.

    Science.gov (United States)

    Lilienthal, Hellmuth; Dieter, Hermann H; Hölzer, Jürgen; Wilhelm, Michael

    2017-03-02

    The detection of perfluoroalkyl substances (PFAS) in surface and drinking water from various countries raised the attention to the presence of these chemicals in environmental probes and led to several regulatory actions to limit exposure in human beings. There was particular concern about perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), due to their former wide-spread use. Recently, several institutions published revisions of former regulatory or recommended maximum concentrations in drinking water and food, which are markedly lower than the former values. The present short overview describes the current regulations for PFAS and compares them with the outcome of several experimental studies in laboratory animals at low-level exposure to PFOA and PFOS. In addition, regulations for short-chain PFAS are presented which, due to lack of toxicological information, are evaluated according to the concepts of Threshold of Toxicological Concern (TTC) or the Health-related Indication Values (HRIV).

  20. Assaying Organochlorines in archived serum for a large, long-term cohort: Implications of combining assay results from multiple laboratories over time

    Science.gov (United States)

    Sholtz, Robert I.; McLaughlin, Katherine R.; Cirillo, Piera M.; Petreas, Myrto; Park, June-Soo; Wolff, Mary S.; Factor-Litvak, Pam; Eskenazi, Brenda; Krigbaum, Nickilou; Cohn, Barbara A.

    2011-01-01

    Conserving irreplaceable, archived serum samples may sometimes conflict with the objective of minimizing measurement error due to laboratory effects. We sought to determine whether we could successfully combine assay results for DDT-related compounds and polychlorinated biphenyls (PCBs) in serum from the same birth cohort obtained from different laboratories over time. Using the Child Health and Development Studies (CHDS) serum archive, we compared variability for assays of a quality control pool to variability for assays of subject serum. The quality control pool was created from native archived serum samples that were pooled, then aliquoted, blinded and inserted pair-wise into assay batches along with the subject serum for 5 studies using CHDS samples conducted over a 13 year period by three different laboratories. We found that the variability between laboratory and over time within laboratory was small relative to inter-individual variability for p,p′-DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane), p,p′-DDE (1,1′-dichloro-2,2′-bis(p-chlorophenyl)ethylene) and o,p′-DDT (1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl)-ethane). Results were also consistent for most PCB congeners which were detectable in 85% or more of samples. Our results suggest that it is possible to combine assays for DDT and PCB congeners measured at positive levels as they are accumulated for cohort subjects without risking meaningful misclassification due to variation stemming from laboratory or time period. This has significant implications for future study costs, conservation of irreplaceable archived samples and for leveraging past investments for future research. For PCB congeners with very low levels, findings caution against pooling of assays without further exploration. PMID:21333355

  1. Abnormal protein aggregationand neurodegenerativediseases

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Abnormal protein aggregation or amyloid is the major cause ofmany neurodegenerative disorders. The present review focuses on the correlation between sequence and structure features of proteins related to the diseases and abnormal protein aggregation. Recent progress has improved our knowledge on understand-ing the mechanism of amyloid formation. We suggest a nucleation model for ordered protein aggregation, which can also explain pathogenesis mechanisms of these neurodegenerative diseases in vivo.

  2. 染色体核型异常患者全基因组芯片扫描结果分析%Analysis on the results of chromosomal microarray in patients with chromosome abnormality

    Institute of Scientific and Technical Information of China (English)

    姚如恩; 傅启华; 余永国

    2014-01-01

    目的:利用全基因组芯片扫描技术对染色体核型检测结果异常的患者样本进行重复检测分析,验证并确认患者染色体的具体核型。方法利用基因分型芯片技术对9例临床表型皆为智力落后合并多发畸形,且核型结果异常的样本进行检测分析,比较两种技术之间结果相符程度,通过芯片平台结果来验证染色体核型技术的准确性,同时分析其临床适用性。结果染色体核型结果和全基因组芯片分析技术的结果完全符合的为2例Turner综合征患者,均为嵌合型;3例染色体核型结果阳性患者,全基因组芯片分析结果为阴性,其中2例为随体增加,1例为染色体内倒位;4例涉及染色体片段大小不同的缺失和复制的患者,核型结果和全基因组芯片结果差异较大,并且核型检测结果与患者实际核型相差较大。结论染色体核型技术在用于以往认定的适应症如智力落后、多发畸形的检测中,检测的准确性相对全基因组芯片技术较低,在明确定位染色体缺失和复制大小及位置的能力上有明显的不足,但对于检测染色体平衡性结构性变化的作用不能被芯片所取代。%Objective To analyze the results of patients with chromosome abnormality by chromosomal microarray, and validate and depict patient′s karyotype.Methods Genotyping array was applied to assess in 9 patients with hypophrenia and deformity.The samples with karyotype analysis abnormality were determined.Comparison between karyotyping and microarray analysis was considered to validate the clinical utility of chromosomal microarray analysis. Results Two Turner syndrome patients had completely concordant results from karyotype analysis and chromosomal microarray.Three patients showed abnormal karyotyping results (2 patients with excess trabant and 1 patients with chromosomal inversion) were detected negative with chromosomal microarray

  3. Abnormalities in eelpout Zoarces viviparus upon chemical exposure.

    Science.gov (United States)

    Brande-Lavridsen, Nanna; Korsgaard, Bodil; Dahllöf, Ingela; Strand, Jakob; Tairova, Zhanna; Bjerregaard, Poul

    2013-12-01

    Elevated frequencies of abnormal embryos in female eelpout Zoarces viviparus have been demonstrated in Danish, Swedish and German monitoring programmes at certain geographic locations with high levels of anthropogenic input. Pollutants present in areas with high malformation frequencies were selected and tested in a controlled laboratory experiment for their potential to induce abnormalities among eelpout embryos upon injection into pregnant eelpout. Tributyltin, 2,3,7,8-tetrachlorodibenzo-p-dioxin, pyrene, nonylphenol, 2,2',4,4'-tetrabromophenylether and heptadecafluorooctanesulfonic acid were tested, either individually or combined. Generally, the chemicals were transferred to eggs and/or embryos. Some of the exposures increased the proportion of broods with more than 10% abnormal or 5% malformed embryos, although the average percentages of abnormal development were not affected. Spinal, cranial and eye deformities were evident, similarly to what is seen in nature. Some of the exposures resulted in increased percentages of females with as well a low reproductive capacity as embryos with a low condition index.

  4. Advantages and limitations of anticipating laboratory test results from regression- and tree-based rules derived from electronic health-record data.

    Directory of Open Access Journals (Sweden)

    Fahim Mohammad

    Full Text Available Laboratory testing is the single highest-volume medical activity, making it useful to ask how well one can anticipate whether a given test result will be high, low, or within the reference interval ("normal". We analyzed 10 years of electronic health records--a total of 69.4 million blood tests--to see how well standard rule-mining techniques can anticipate test results based on patient age and gender, recent diagnoses, and recent laboratory test results. We evaluated rules according to their positive and negative predictive value (PPV and NPV and area under the receiver-operator characteristic curve (ROC AUCs. Using a stringent cutoff of PPV and/or NPV≥0.95, standard techniques yield few rules for sendout tests but several for in-house tests, mostly for repeat laboratory tests that are part of the complete blood count and basic metabolic panel. Most rules were clinically and pathophysiologically plausible, and several seemed clinically useful for informing pre-test probability of a given result. But overall, rules were unlikely to be able to function as a general substitute for actually ordering a test. Improving laboratory utilization will likely require different input data and/or alternative methods.

  5. HER2 status in gastric and gastroesophageal junction cancer assessed by local and central laboratories: Chinese results of the HER-EAGLE study.

    Directory of Open Access Journals (Sweden)

    Dan Huang

    Full Text Available Trastuzumab has been approved for human epidermal growth factor receptor 2 (HER2-positive advanced gastric and gastroesophageal junction cancers (GC and GJC in combination with chemotherapy. The aim of this HER2 early/advanced gastric epidemiology (HER-EAGLE study was to evaluate the frequency of HER2 over-expression and to evaluate agreement on HER2 status assessment in GC and GJC patients in local laboratories versus a central laboratory in China. Tumor samples from 734 GC or GJC patients who were enrolled at 11 different hospitals in China were examined. HER2 status was assessed by immunohistochemistry (IHC, and followed by dual-color silver-enhanced in Situ hybridization (DSISH in IHC 2+ cases. Clinicopathologic characteristics were collected from all of the patients. HER2-positive tumors were identified in 12.0% (88/734 of the GC and GJC cases. There were significantly higher rates of HER2 positivity in patients with GJC (GJC: 18.1%, GC: 9.7%, P=0.002, and intestinal-type cancers using the Lauren classification (intestinal: 23.6%, diffuse/mixed: 4.3%, P<0.0001. No significant difference in HER2 positivity was identified between resection and biopsy samples, or between early and advanced disease stages. The agreement between local laboratories and the central laboratory on HER2 status scoring was good (kappa=0.86. The main reason of HER2 status discordance between local and the central laboratories was IHC result mis-interpretation in local laboratories. These results suggest that IHC followed by DSISH testing is an accurate and cost-effective procedure in China.

  6. 不育男性Y染色体长度异常的精液质量%Effect of abnormality Y chromosome and sperm quality on the result of insisted reproductive techniques

    Institute of Scientific and Technical Information of China (English)

    谢伟; 秦雯; 莫定敢; 陈美佳; 吕福通; 覃爱平

    2016-01-01

    Objective To investigate the effect of abnormality Y chromosome and sperm quality on the clinical result of insisted reproductive technique (ART). Methods We retrospectively analyzed the effect of ab-normality Y chromosome and sperm quality on embryo quality/result of pregnancy of patients undergoing ART. In-fertile men with normality Y chromosome (n=436) and with abnormality Y chromosome (n =146) from Jan 2010 to Dec 2010 were enrolled in this study. The infertile men were divided into four groups: the normality Y chromo-some plus teratozoospermia group , the normality Y chromosome plus normal sperm quality group , the abnormality Y chromosome plus teratozoospermia group, the abnormality Y chromosome plus normal sperm quality group. Embryo quality and the results of clinic pregnancy outcome were analyzed among the four groups. Results Fertilization rate of the four groups was 73.4%, 69.0%, 70.9% and 68%, respectively (P < 0.05). No significant differences were found in thecleavage rate, optimal embryo rate and pregnancy outcome among the four groups. Conclusions Both abnormality Y chromosome length and teratozoospermia may be affect the fertility rate of the infertile men undergoing IVF/ICSI.%目的:了解在辅助生殖技术(insisted reproductive techniques,ART)中 Y 染色体长度异常患者的精液质量情况,并按不同精液质量分组,讨论其胚胎质量、临床妊娠结局及意义。方法:通过回顾性分析2010年1月1日至2010年12月31日在中心进行辅助生殖助孕的患者共2739周期,染色体检查682例,其中染色体正常436(63.93%),合并畸形精子症196例,染色体长度异常共146例(Y =22,139例;Y =18,7例),占5.33%,合并畸形精子症73例。将患者分为染色体长度正常组和染色体长度异常组,再分精子形态正常组、精子形态异常组2个亚组,共4组,即染色体长度正常且精子形态正常组(组1),染色体长度正

  7. Results of Laboratory Testing of 15 Cookstove Designs in Accordance with the ISO/IWA Tiers of Performance.

    Science.gov (United States)

    Still, Dean; Bentson, Samuel; Li, Haixi

    2015-03-01

    The widespread adoption and sustained use of modern cookstoves has the potential to reduce harmful effects to climate, health, and the well-being of approximately one-third of the world's population that currently rely on biomass fuel for cooking and heating. In an effort to understand and develop cleaner burning and more efficient cookstoves, 15 stove design and fuel/loading combinations were evaluated in the laboratory using the International Workshop Agreement's five-tiered (0-4) rating system for fuel use and emissions. The designs evaluated include rocket-type combustion chamber models including reduced firepower, sunken pots, and chimneys (three stoves); gasifier-type combustion chambers using prepared fuels in the form of wood pellets (four stoves); forced draft stoves with a small electric fan (five stoves); and a single insulated charcoal stove with preheated secondary air. It was found that a charcoal burning stove was the only stove to meet all the Tier 4 levels of performance. Achieving over 40% thermal efficiency at high power was made possible by reducing firepower and gaps around the pot, although batch-fed stoves generally do not "turn down" for optimal low power performance. While all stoves met Tier 4 for carbon monoxide, only stoves equipped with electrical fans reduced respirable particulate matter to Tier 4 levels. Finally, stoves with chimneys and integrated pots were fuel efficient and virtually eliminated indoor emissions. It is hoped that these design techniques will be useful in further development and evolution of high-performance cookstove designs.

  8. Congenital abnormalities in methylmercury poisoning

    Energy Technology Data Exchange (ETDEWEB)

    Gilani, S.H.

    1975-04-01

    This study was undertaken to determine the teratogenic potential of methylmercury on chick embryogenesis. Methylmercuric chloride was dissolved in sodium bicarbonate (0.2%) and administered to the chick embryos at doses ranging from 0.0009 to 0.010 mg per egg. The injections were made at days 2 and 3 on incubation (Groups A and B). All the embryos including controls were examined on the 7th day of incubation. Methylmercury poisoning was observed to be both embryolethal and teratogenic. Within the two groups, embryolethality was higher in Group A. The following congenital abnormalities were observed: exencephaly, shortened and twisted limbs, microphthalmia, shortened and twisted neck, beak abnormalities, everted viscera, reduced body size and hemorrhage all over the body. Exencephaly and limb abnormalities were very common. No differences in the incidence and types of gross abnormalities within both the groups (A and B) were noted. The incidence of malformations among the controls was low. The results of present investigation show that methylmercury poisoning is both embryolethal and teratogenic to early chick embryogenesis. (auth)

  9. Abnormal Raman spectral phenomenon of silicon nanowires

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The Raman spectra of two one-dimensional silicon nanowire samples with different excitation wavelengths were measured and an abnormal phenomenon was discovered that the Raman spectral features change with the wavelengths of excitation. Closer analysis of the crystalline structure of samples and the changes in Raman spectral features showed that the abnormal behavior is the result of resonance Raman scattering selection effect.

  10. An Abnormal Vibrational Mode of Torsion Pendulum

    Institute of Scientific and Technical Information of China (English)

    赵亮; 涂英; 顾邦明; 胡忠坤; 罗俊

    2003-01-01

    In the experiment for the determination of the gravitational constant G, we found an abnormal vibrational mode of the torsion pendulum. The abnormal mode disappeared as a magnetic damper was introduced to the torsion pendulum system. Our experimental results also show that the magnetic damper can be used to suppress the high frequency vibrational noises to torsion pendulums effectively.

  11. Evaluation of the results of acute viral gastroenteritis data in Refik Saydam National Public Health Agency, Virology Reference and Research Laboratory in 2009

    Directory of Open Access Journals (Sweden)

    Nurhan ALBAYRAK

    2011-01-01

    Full Text Available Objective: Norovirus, Rotavirus, Adenovirus and Astrovirus are responsible for most non-bacterial acute gastroenteritis. The incidence of these viral agents in Turkey is not well known. In this study, it was aimed to document the viral etiology of the stool samples which were send to Refik Saydam National Public Health Agency (RSNPHA, Virology Reference and Research Laboratory for investigation of viral acute gastroenteritis agents. Method: A total of 147 stool samples from 11 different provinces were send to the Virology Laboratory for Reference and Research of RSNPHA in 2009. Samples were collected from patients admitted because of acute gastroenteritis and from the cases with the signs of illness at different times of the year and sent by the Provincial Health Directorates to our laboratory. The samples were examined in the laboratory using the commercial multiplex real-time PCR kit for norovirus genotype I, norovirus genotype II, rotavirus, adenovirus and astrovirus. Results: 65 (44.2 % samples were found to be positive at least for one viral agent and 10 (6.8 % samples for more than one viral agent. Norovirus (particularly genotype II infections were detected as the most prevalent viral agent in acute gastroenteritis patients in this period. Rotavirus infections were determined as the second most common infection after norovirus infections. Adenovirus infections have been found to be the least prevalent agent in the laboratory. Conclusion: Results of this study showed that norovirus genotype II has been more commonly responsible for acute diarrhea than the other viral pathogens. The viral agents we have studied should be considered as pathogens that can be seen in all seasons. Viral factors should not be underestimated as the cause of acute gastroenteritis; additionally it should be noted that acute gastroenteritis could be caused by coinfection of viral agents.

  12. Laboratory Tests Turnaround Time in Outpatient and Emergency Patients in Nigeria: Results of A Physician Survey on Point of Care Testing

    Directory of Open Access Journals (Sweden)

    Bolodeoku J

    2017-05-01

    Full Text Available Laboratory analytical turnaround time is a well-recognised indicator of how well a laboratory is performing and is sometimes regarded as the benchmark for laboratory performance. Methods: Total 104 doctors in public and private health institutions in Nigeria, spread across all six geo-political zones participated in survey requesting information on their experience with laboratory turnaround times in emergency situations (emergency room, special care baby unit, intensive care unit, dialysis unit and outpatient situations (general medicine and diabetes. Results: The average turnaround time in hours was 5.12, 8.35, 7.32 and 8.33 for the emergency room, special care baby unit, intensive care unit and dialysis unit, respectively. For the outpatient situations, the average turnaround time in hours was 10.74 and 15.70 hours for the diabetes and general medical outpatients. The median range (hours and modal range (hours for: the emergency room was 2-4 and <2; the special care baby unit was 4-8 and 4-8; the intensive care unit was 2-4 and 2-4; the dialysis unit was 4-8 and 4-8. The median range (hours and modal range (hours for: the general outpatient clinic was 12-24 and 12-24; the diabetic clinic was 4-8 and 12-24 hours. Conclusion: These turnaround time results are quite consistent with published data from other countries. However, there is some measure of improvement that is required in some areas to reduce the laboratory turnaround in the emergency situations. This could be overcome with the introduction of more point of care testing devices into emergency units.

  13. [Hair shaft abnormalities].

    Science.gov (United States)

    Itin, P H; Düggelin, M

    2002-05-01

    Hair shaft disorders may lead to brittleness and uncombable hair. In general the hair feels dry and lusterless. Hair shaft abnormalities may occur as localized or generalized disorders. Genetic predisposition or exogenous factors are able to produce and maintain hair shaft abnormalities. In addition to an extensive history and physical examination the most important diagnostic examination to analyze a hair shaft problem is light microscopy. Therapy of hair shaft disorders should focus to the cause. In addition, minimizing traumatic influences to hair shafts, such as dry hair with an electric dryer, permanent waves and dyes is important. A short hair style is more suitable for such patients with hair shaft disorders.

  14. Communication of laboratory data and diagnostic test results to hospitalized patients: a study of preferences and recall

    Directory of Open Access Journals (Sweden)

    Athar MW

    2016-07-01

    Full Text Available Muhammad W Athar,1 Christine Mativo,1 Regina Landis,2 Scott M Wright1 1Department of Medicine, Johns Hopkins University School of Medicine, 2Division of Hospital Medicine, Johns Hopkins Bayview Medical Center, Baltimore, MD, USA Objective: To assess patients’ preferences with respect to different methods of receiving test results while they were hospitalized and to determine whether the different modes of communication of the test results were associated with better recall. Methods: Five discrete test results were shared with adult inpatients on general medicine service (blood pressure, white blood cell count, hematocrit, creatinine, and chest X-ray. The information was delivered by a physician in one of three ways: 1 verbally, 2 explained with a print out of the results, or 3 described while showing results on a computer monitor (electronic. The same physician returned within 3 hours to assess recall and satisfaction with the way patients received their results. Results: All the patients (100% receiving their results in written format were satisfied with the mode of communication as compared to electronic format (86% or verbally (79% (P=0.02. Fifty percent of patients in the computer format group could recall four or more test results at the follow-up, as compared to 43% in printed group and 24% who were informed of their results verbally (P=0.35. Conclusion: Patients most appreciated receiving test results in written form while in the hospital, and this delivery method was as good as any other method with respect to recall. Keywords: patient preference, patient satisfaction, patient-centered care

  15. 妊娠晚期血小板参数及凝血指标异常结果分析%Analysis on the abnormal results of platelet parameters and coagulation indexes during the third trimester of pregnancy

    Institute of Scientific and Technical Information of China (English)

    梁绮华; 肖燕青; 杨红玲

    2012-01-01

    目的:总结分析妊娠晚期导致孕妇血小板及凝血检测结果异常的原因、发生频率以及检测方法上要注意的问题.方法:以2008年9月~2009年8月间的住院孕妇作为研究对象,按妊娠并发症分类,以正常晚期妊娠妇女作为对照,分析血小板参数、凝血常规四项(PT、APTT、TT、FIB).结果:①妊娠高血压综合征(PIH)组和妊娠糖尿病(GDM)组的血小板平均体积(MPV)值高于对照组;②妊高征组血小板降低、MPV升高、凝血指标未见明显异常.③妊娠糖尿病组的PT、APIT、FIB与对照组比较有显著性差异.④妊娠晚期孕妇发生血小板减少的原因以妊娠生理性原因和重度妊高征为主(58.4%和20.7%),其他原因还包括:双胎妊娠、母儿血型不合、EDTA依赖性假性PLT减少.结论:多种妊娠相关性疾病可引起妊娠晚期孕妇的血小板减少,血小板参数异常,同时需要注意鉴别EDTA依赖性假性PLT减少,避免误诊.%Objectives To summarize and analyze the causes and frequencies of abnormal results of platelet detection and coagulation test, and the problems needing attention for detection methods among the pregnant women during the third trimester of pregnancy. Methods: The pregnant women who hospitalized in the hospital from September 2008 to August 2009 were selected as study objects , then they were classified according to pregnant complications; the normal pregnant women during the third trimester of pregnancy were selected as control group, the platelet parameters and four coagulation indexes, including prothrombin time (FT), activated partial thrombo-plastin time (APTT) , thrombin time (TT) and fibrinogen, were analyzed. Results; The mean platelet volumes (MPV) in hypertensive disorder complicating pregnancy ( HDCP) group and gestational diabetes mellitus (GDM) group were significantly higher than that in control group. In HDCP group, the count of platelet decreased, MPV increased, there was no

  16. Results of a European inter-laboratory comparison study on the determination of EU priority polycyclic aromatic hydrocarbons (PAHs) in edible vegetable oils.

    Science.gov (United States)

    Simon, Rupert; Gomez Ruiz, José Angel; von Holst, Christoph; Wenzl, Thomas; Anklam, Elke

    2008-06-01

    A collaborative study on the analysis for 15 + 1 EU priority PAHs in edible oils was organised to investigate the state-of-the-art of respective analytical methods. Three spiked vegetable oils, one contaminated native sunflower oil, and one standard solution were investigated in this study. The results of 52 laboratories using either high-performance liquid chromatography with fluorescence detection or gas chromatography with mass-selective detectors were evaluated by application of robust statistics. About 95% of the laboratories were able to quantify benzo[a]pyrene together with five other PAHs included in the commonly known list of 16 US-EPA PAHs. About 80% of the participants also quantified seven additional PAHs in most samples, two of which were benzo[b]fluoranthene and benzo[k]fluoranthene, which were also known from the EPA list. Only about 50% of the participants quantified cyclopenta[cd]pyrene, benzo[j]fluoranthene, and benzo[c]fluorene. The robust relative standard deviations of the submitted results without discrimination between the methods applied ranged between 100% for 5-methylchrysene in spiked olive oil and 11% for the same analyte in spiked sunflower oil. The results clearly showed that for these analytes the methods of analysis are not yet well established in European laboratories, and more collaborative trials are needed to promote further development and to improve the performances of the respective methods.

  17. Chromosomal abnormalities and autism

    African Journals Online (AJOL)

    Farida El-Baz

    2015-06-19

    Jun 19, 2015 ... Received 19 April 2015; accepted 11 May 2015 ... Methods: This cross sectional study was conducted at the Child Psychiatry Clinic, ... Males are affected more than females, only one case had ... communication, repetitive behavior, abnormal movement ... course, age, sex and consanguinity of the patients.

  18. Cortical Abnormalities in ADHD

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2003-12-01

    Full Text Available Grey-matter abnormalities at the cortical surface and regional brain size were mapped by high-resolution MRI and surface-based, computational image analytical techniques in a group of 27 children and adolescents with attention deficit hyperactivity disorder (ADHD and 46 controls, matched by age and sex, at the University of California at Los Angeles.

  19. Neurological abnormalities predict disability

    DEFF Research Database (Denmark)

    Poggesi, Anna; Gouw, Alida; van der Flier, Wiesje

    2014-01-01

    To investigate the role of neurological abnormalities and magnetic resonance imaging (MRI) lesions in predicting global functional decline in a cohort of initially independent-living elderly subjects. The Leukoaraiosis And DISability (LADIS) Study, involving 11 European centres, was primarily aimed...

  20. Lacrimal system abnormalities.

    Science.gov (United States)

    Moore, B D

    1994-03-01

    This report outlines several of the more important abnormalities of the lacrimal system in infants and young children. Although rare, alacrima can be a very difficult clinical problem to treat. The most common cause of alacrima is the Riley-Day syndrome. Nasolacrimal duct obstruction is a very common anomaly in children. The clinical appearance and treatment of this disorder are discussed.

  1. Most but not all laboratories can detect the recently emerged Neisseria gonorrhoeae porA mutants - results from the QCMD 2013 N. gonorrhoeae external quality assessment programme.

    Science.gov (United States)

    Luijt, D; Di Lorenzo, C; van Loon, A M; Unemo, M

    2014-02-27

    We describe the results of the Quality Control for Molecular Diagnostics 2013 Neisseria gonorrhoeae external quality assessment programme that included an N. gonorrhoeae strain harbouring an N. meningitidis porA gene which causes false-negative results in molecular diagnostic assays targeting the gonococcal porA pseudogene. Enhanced awareness of the international transmission of such gonococcal strains is needed to avoid false-negative results in both in-house and commercial molecular diagnostic assays used in laboratories worldwide, but particularly in Europe.

  2. Laboratory studies of H2SO4/H2O binary homogeneous nucleation from the SO2+OH reaction: evaluation of the experimental setup and preliminary results

    Directory of Open Access Journals (Sweden)

    F. R. Kameel

    2008-04-01

    Full Text Available We have developed a new laboratory nucleation setup to study binary homogeneous nucleation (BHN of sulphuric acid and water (H2SO4/H2O. Here we provide a detailed evaluation of this new experimental setup and also discuss our preliminary results by comparing with other laboratory studies. H2SO4 is produced from the SO2+OH →HSO3 reaction and OH radicals are produced from water vapor UV absorption. The residual H2SO4 concentrations ([H2SO4] are measured at the end of the fast flow nucleation reactor with a chemical ionization mass spectrometer. The measured BHN rates (J ranged from 0.02 and 550 cm−3 s−1 at the residual [H2SO4] from 108 to 1010 cm−3, a temperature of 288 K and relative humidity (RH from 6 to 23%; J increased with increasing [H2SO4] and RH. J also showed a power dependence on [H2SO4] with the exponential power of 3 to 8. These results are consistent with other laboratory studies under similar [H2SO4] and RH, but different from atmospheric field observations which showed that particle number concentrations are often linearly dependent on [H2SO4]. Both particle sizes and number concentrations increased with increasing [H2SO4], RH, and nucleation time, consistent with the predictions from nucleation theories. Particle growth rates were estimated between 28 to 127 nm h−1, much higher than those seen from atmospheric field observations, because of the higher [H2SO4] used in our study. While these experimental results demonstrate a validation of our laboratory setup, there are also technical difficulties associated with nucleation studies, including wall loss and H2SO4 measurements.

  3. Nail abnormalities in patients with vitiligo*

    Science.gov (United States)

    Topal, Ilteris Oguz; Gungor, Sule; Kocaturk, Ozgur Emek; Duman, Hatice; Durmuscan, Mustafa

    2016-01-01

    Background Vitiligo is an acquired pigmentary skin disorder affecting 0.1-4% of the general population. The nails may be affected in patients with an autoimmune disease such as psoriasis, and in those with alopecia areata. It has been suggested that nail abnormalities should be apparent in vitiligo patients. Objective We sought to document the frequency and clinical presentation of nail abnormalities in vitiligo patients compared to healthy volunteers. We also examined the correlations between nail abnormalities and various clinical parameters. Methods This study included 100 vitiligo patients and 100 healthy subjects. Full medical histories were collected from the subjects, who underwent thorough general and nail examinations. All nail changes were noted. In the event of clinical suspicion of a fungal infection, additional mycological investigations were performed. Results Nail abnormalities were more prevalent in the patients (78%) than in the controls (55%) (p=0.001). Longitudinal ridging was the most common finding (42%), followed by (in descending order): leukonychia, an absent lunula, onycholysis, nail bed pallor, onychomycosis, splinter hemorrhage and nail plate thinning. The frequency of longitudinal ridging was significantly higher in patients than in controls (p<0.001). Conclusions Nail abnormalities were more prevalent in vitiligo patients than in controls. Systematic examination of the nails in such patients is useful because nail abnormalities are frequent. However, the causes of such abnormalities require further study. Longitudinal ridging and leukonychia were the most common abnormalities observed in this study. PMID:27579738

  4. [CHROMOSOMAL ABNORMALITIES IN PATIENTS WITH INFERTILITY].

    Science.gov (United States)

    Pylyp, L Y; Spinenko, L O; Verhoglyad, N V; Kashevarova, O O; Zukin, V D

    2015-01-01

    To assess the frequency and structure of chromosomal abnormalities in patients with infertility, a retrospective analysis of cytogenetic studies of 3414 patients (1741 females and 1673 males), referred to the Clinic of reproductive medicine "Nadiya" from 2007 to 2012, was performed. Chromosomal abnormalities were detected in 2.37% patients: 2.79% in males and 1.95% in females. Balanced structural chromosomal abnormalities prevailed over numerical abnormalities and corresponded to 80.2% of all chromosomal abnormalities detected in the studied group. Sex chromosome abnormalities made up 23.5% of chromosomal pathology (19/81) and included gonosomal aneuploidies in 84% of cases (16/19) and structural abnormalities of chromosome Y in 16% of cases (3/19). The low level sex chromosome mosaicism was detected with the frequency of 0.55%. Our results highlight the importance of cytogenetic studies in patients seeking infertility treatment by assisted reproductive technologies, since an abnormal finding not only provide a firm diagnosis to couples with infertility, but also influences significantly the approach to infertility treatment in such patients.

  5. Brief overview of the creation of the synchrocyclotron at the hydrotechnical laboratory of the USSR Academy of Sciences and first results of physical studies: Review of archival documents

    Science.gov (United States)

    Kiselev, G. V.; Rusakovich, N. A.

    2012-07-01

    A review of the archive's documents on the main stages of the creation of the synchrocyclotron at the Hydrotechnical Laboratory (now known as the Dzhelepov Laboratory of Nuclear Problems) is presented. The review is based on minutes of the Special Committee and Scientific and Technical Council (NTS), which belongs to the First Chief Directorate (PGU) concerning the era of the Soviet atomic project (1945-1953). The analysis of these minutes has enabled us to prepare a chronicle of events that deal with the creation of the synchrocyclotron, as well as the discussion of the project and its place of construction, the research and development program, and so on. The minutes of two NTS meetings dated May 5 and 12, 1952 are devoted to discussing the results the research and development program in 1950-1951. The minutes of NTS meetings have not been published previously.

  6. Prototype integration of protein electrophoresis laboratory results in an information warehouse to improve workflow and data analysis.

    Science.gov (United States)

    Liu, Jianhua; Silvey, Scott A; Bissell, Michael G; Saltz, Joel H; Kamal, Jyoti

    2006-01-01

    This poster demonstrates our efforts to enhance workflow and clinical analysis of protein electrophoresis (PEP) data through integration with the Information Warehouse (IW) at The Ohio State University Medical Center (OSUMC). A new desktop application has been developed with the aim of enabling more efficient and accurate gel analysis by clinical pathologists. This tool gives the pathologists the ability to perform their analysis conveniently from anywhere on the OSUMC network along with the aid of numerical analysis algorithms, image enhancement techniques, and access to historical PEP results for the given patient.

  7. Results of low energy background measurements with the Liquid Scintillation Detector (LSD) of the Mont Blanc Laboratory

    Science.gov (United States)

    Aglietta, M.; Badino, G.; Bologna, G. F.; Castagnoli, C.; Fulgione, W.; Galeotti, P.; Saavedra, O.; Trinchero, G. C.; Vernetto, S.; Dadykin, V. L.

    1985-01-01

    The 90 tons liquid scintillation detector (LSD) is fully running since October 1984, at a depth of 5,200 hg/sq cm of standard rock underground. The main goal is to search for neutrino bursts from collapsing stars. The experiment is very sensitive to detect low energy particles and has a very good signature to gamma-rays from (n,p) reaction which follows the upsilon e + p yields n + e sup + neutrino capture. The analysis of data is presented and the preliminary results on low energy measurements are discussed.

  8. Different chromosome Y abnormalities in a case with short stature.

    Science.gov (United States)

    Balkan, Mahmut; Fidanboy, Mehmet; Özbek, M Nuri; Alp, M Nail; Budak, Turgay

    2012-12-01

    We report a case with different chromosome Y abnormalities. Case was an 11-year-old boy, who was diagnosed with short stature, referred to laboratory of human medical genetics laboratory for genetic evaluation. Chromosomal analysis of the case was carried out on peripheral blood lymphocyte culture. Classic cytogenetic analysis (G and C banding) was confirmed by using fluorescence in situ hybridization analysis (FISH) technique. Cytogenetic and FISH analysis showed a mosaic 46,X,i(Yq)/45,X/47,X,i(Yq)x2/47,XYY karyotype. Case, which was found interesting due to its rarity, is discussed with its clinical features and cytogenetic results, in the light of relevant source information. This case underlines the importance of karyotyping patients with unexplained short stature. This clinical report also will be helpful in defining the phenotypic range associated with these karyotypes.

  9. Results from nationwide hepatitis B serosurvey in Cambodia using simple and rapid laboratory test: implications for National Immunization Program.

    Science.gov (United States)

    Soeung, Sann Chan; Rani, Manju; Huong, Vu; Sarath, Savay; Kimly, Chea; Kohei, Toda

    2009-08-01

    Chronic liver infection and cancer in the western Pacific region is disproportionate to the population globally. This study provides the first nationwide estimates of hepatitis B surface antigen (HBsAg) seroprevalence in Cambodia among children five year of age. Using a simple and rapid test for HBsAg and multi-stage stratified cluster sampling design, we estimated HBsAg seroprevalence to be 3.5% (95% confidence interval = 2.4-4.8%) among five-year old children. Triangulating the results with other studies, we demonstrate the importance of interrupting perinatal transmission and one-time catch-up vaccination of older children born before nationwide introduction of vaccination for effective hepatitis B control in Cambodia and for reaching the disease control goal of less than 2% chronic infection rates among children > or = 5 years of age. The results demonstrate the feasibility of conducting nationwide serosurveys using simple and rapid tests to evaluate the impact of hepatitis B vaccination programs in lieu of standard enzyme-linked immunosorbent assays.

  10. First results from a Dark Matter search with liquid Argon at 87 K in the Gran Sasso Underground Laboratory

    CERN Document Server

    Benetti, P; Adamo, F; Baibussinov, B; Baldo-Ceolin, M; Belluco, M; Calaprice, F; Calligarich, E; Cambiaghi, M; Carbonara, F; Cavanna, F; Centro, Sandro; Cocco, A G; Di Pompeo, F; Ferrari, N; Fiorillo, G; Galbiati, C; Gallo, V; Grandi, L; Ianni, A; Mangano, G; Meng, G; Montanari, C; Palamara, O; Pandola, L; Pietropaolo, F; Raselli, G L; Rossella, M; Rubbia, C; Szelc, A M; Ventura, Sandro; Vignoli, C; 10.1016/j.astropartphys.2007.08.002

    2008-01-01

    A new method of searching for dark matter in the form of weakly interacting massive particles (WIMP) has been developed with the direct detection of the low energy nuclear recoils observed in a massive target (ultimately many tons) of ultra pure Liquid Argon at 87 K. A high selectivity for Argon recoils is achieved by the simultaneous observation of both the VUV scintillation luminescence and of the electron signal surviving columnar recombination, extracted through the liquid-gas boundary by an electric field. First physics results from this method are reported, based on a small 2.3 litre test chamber filled with natural Argon and an accumulated fiducial exposure of about 100 kg x day, supporting the future validity of this method with isotopically purified 40Ar and for a much larger unit presently under construction with correspondingly increased sensitivities.

  11. Plans and Preliminary Results of Fundamental Studies of Ice Crystal Icing Physics in the NASA Propulsion Systems Laboratory

    Science.gov (United States)

    Struk, Peter; Tsao, Jen-Ching; Bartkus, Tadas

    2017-01-01

    This paper describes plans and preliminary results for using the NASA Propulsion Systems Lab (PSL) to experimentally study the fundamental physics of ice-crystal ice accretion. NASA is evaluating whether this facility, in addition to full-engine and motor-driven-rig tests, can be used for more fundamental ice-accretion studies that simulate the different mixed-phase icing conditions along the core flow passage of a turbo-fan engine compressor. The data from such fundamental accretion tests will be used to help develop and validate models of the accretion process. This paper presents data from some preliminary testing performed in May 2015 which examined how a mixed-phase cloud could be generated at PSL using evaporative cooling in a warmer-than-freezing environment.

  12. Automated detection of external ventricular and lumbar drain-related meningitis using laboratory and microbiology results and medication data.

    Directory of Open Access Journals (Sweden)

    Maaike S M van Mourik

    Full Text Available OBJECTIVE: Monitoring of healthcare-associated infection rates is important for infection control and hospital benchmarking. However, manual surveillance is time-consuming and susceptible to error. The aim was, therefore, to develop a prediction model to retrospectively detect drain-related meningitis (DRM, a frequently occurring nosocomial infection, using routinely collected data from a clinical data warehouse. METHODS: As part of the hospital infection control program, all patients receiving an external ventricular (EVD or lumbar drain (ELD (2004 to 2009; n = 742 had been evaluated for the development of DRM through chart review and standardized diagnostic criteria by infection control staff; this was the reference standard. Children, patients dying <24 hours after drain insertion or with <1 day follow-up and patients with infection at the time of insertion or multiple simultaneous drains were excluded. Logistic regression was used to develop a model predicting the occurrence of DRM. Missing data were imputed using multiple imputation. Bootstrapping was applied to increase generalizability. RESULTS: 537 patients remained after application of exclusion criteria, of which 82 developed DRM (13.5/1000 days at risk. The automated model to detect DRM included the number of drains placed, drain type, blood leukocyte count, C-reactive protein, cerebrospinal fluid leukocyte count and culture result, number of antibiotics started during admission, and empiric antibiotic therapy. Discriminatory power of this model was excellent (area under the ROC curve 0.97. The model achieved 98.8% sensitivity (95% CI 88.0% to 99.9% and specificity of 87.9% (84.6% to 90.8%. Positive and negative predictive values were 56.9% (50.8% to 67.9% and 99.9% (98.6% to 99.9%, respectively. Predicted yearly infection rates concurred with observed infection rates. CONCLUSION: A prediction model based on multi-source data stored in a clinical data warehouse could accurately

  13. Laboratory evaluation of the Level TROLL 100 manufactured by In-Situ Inc.: results of pressure and temperature tests

    Science.gov (United States)

    Carnley, Mark V.; Fulford, Janice M.; Brooks, Myron H.

    2013-01-01

    The Level TROLL 100 manufactured by In-Situ Inc. was evaluated by the U.S. Geological Survey (USGS) Hydrologic Instrumentation Facility (HIF) for conformance to the manufacturer’s accuracy specifications for measuring pressure throughout the device’s operating temperature range. The Level TROLL 100 is a submersible, sealed, water-level sensing device with an operating pressure range equivalent to 0 to 30 feet of water over a temperature range of −20 to 50 degrees Celsius (°C). The device met the manufacturer’s stated accuracy specifications for pressure within its temperature-compensated operating range of 0 to 50 °C. The device’s accuracy specifications did not meet established USGS requirements for primary water-stage sensors used in the operation of streamgages, but the Level TROLL 100 may be suitable for other hydrologic data-collection applications. As a note, the Level TROLL 100 is not designed to meet USGS accuracy requirements. Manufacturer accuracy specifications were evaluated, and the procedures followed and the results obtained are described in this report. USGS accuracy requirements are routinely examined and reported when instruments are evaluated at the HIF.

  14. The Fourier-Kelvin Stellar Interferometer (FKSI): A Progress Report and Preliminary Results from Our Laboratory Testbed

    Science.gov (United States)

    Berry, Richard; Rajagopa, J.; Danchi, W. C.; Allen, R. J.; Benford, D. J.; Deming, D.; Gezari, D. Y.; Kuchner, M.; Leisawitz, D. T.; Linfield, R.

    2005-01-01

    The Fourier-Kelvin Stellar Interferometer (FKSI) is a mission concept for an imaging and nulling interferometer for the near-infrared to mid-infrared spectral region (3-8 microns). FKSI is conceived as a scientific and technological pathfinder to TPF/DARWIN as well as SPIRIT, SPECS, and SAFIR. It will also be a high angular resolution system complementary to JWST. The scientific emphasis of the mission is on the evolution of protostellar systems, from just after the collapse of the precursor molecular cloud core, through the formation of the disk surrounding the protostar, the formation of planets in the disk, and eventual dispersal of the disk material. FKSI will also search for brown dwarfs and Jupiter mass and smaller planets, and could also play a very powerful role in the investigation of the structure of active galactic nuclei and extra-galactic star formation. We report additional studies of the imaging capabilities of the FKSI with various configurations of two to five telescopes, studies of the capabilities of FKSI assuming an increase in long wavelength response to 10 or 12 microns (depending on availability of detectors), and preliminary results from our nulling testbed.

  15. Laboratory results and status update for Pathfinder, the LINC-NIRVANA NGS ground-layer AO subsystem

    Science.gov (United States)

    Kopon, Derek; Conrad, Al; Bertram, Thomas; Herbst, Tom; Kürster, Martin; Berwein, Juergen; Ragazzoni, Roberto; Farinato, Jacopo; Viotto, Valentina; Bergomi, Maria; Rohloff, Ralf-Rainer; Baumeister, Harald; De Bonis, Fulvio; Hofferbert, Ralph; Brunelli, Alessandro; Arcidiacono, Carmelo; Pott, Jörg-Uwe; Bizenberger, Peter; Briegel, Florian; Meschke, Daniel; Mohr, Lars; Zhang, Xianyu; Kittmann, Frank

    2013-12-01

    The full LINC-NIRVANA instrument will be one of the most complex ground-based astronomical systems ever built. It will consist of multiple subsystems, including two multi-conjugate ground layer AO systems (MCAO) that drive the LBT adaptive secondaries, two mid-high layer AO systems with their own Xynetics 349 actuator DM's, a fringe tracker, and a beam combiner. In order to mitigate risk, we take a modular approach to instrument testing and commissioning by decoupling these subsystems individually. The first subsystem tested on-sky will be one of the ground-layer AO systems, part of a test-bed known as the Pathfinder. The Pathfinder consists of a 12-star pyramid WFS that drives one of the LBT's adaptive secondaries, a support structure known as "The Foot," and the infrared test camera (IRTC), which is used for acquisition and alignment. The 12 guide stars are acquired by moveable arms called "star enlargers," each of which contains its own optical path. The Pathfinder will be shipped from MPIA in Heidelberg, Germany to the LBT mountain lab on Mt. Graham, Arizona in February. The system will be unpacked, assembled in the LBT clean room, and internally optically aligned. We present the results of our system tests, including star enlarger alignment and system alignment. We also present our immediate plans for on-sky closed loop tests on the LBT scheduled for early Fall. Because plans for all ELTs call for ground layer correction, the Pathfinder provides valuable preliminary information not only for the full LINC-NIRVANA system, but also for future advanced MCAO systems.

  16. Volatile Organic Compound (VOC Removal by Vapor Permeation at Low VOC Concentrations: Laboratory Scale Results and Modeling for Scale Up

    Directory of Open Access Journals (Sweden)

    Philippe Moulin

    2011-03-01

    Full Text Available Petroleum transformation industries have applied membrane processes for solvent and hydrocarbon recovery as an economic alternative to reduce their emissions and reuse evaporated components. Separation of the volatile organic compounds (VOCs (toluene-propylene-butadiene from air was performed using a poly dimethyl siloxane (PDMS/α-alumina membrane. The experimental set-up followed the constant pressure/variable flow set-up and was operated at ~21 °C. The membrane is held in a stainless steel module and has a separation area of 55 × 10−4 m². Feed stream was set to atmospheric pressure and permeate side to vacuum between 3 and 5 mbar. To determine the performance of the module, the removed fraction of VOC was analyzed by Gas Chromatography/Flame Ionization Detector (GC/FID. The separation of the binary, ternary and quaternary hydrocarbon mixtures from air was performed at different flow rates and more especially at low concentrations. The permeate flux, permeance, enrichment factor, separation efficiency and the recovery extent of the membrane were determined as a function of these operating conditions. The permeability coefficients and the permeate flux through the composite PDMS-alumina membrane follow the order given by the Hildebrand parameter: toluene > 1,3-butadiene > propylene. The simulated data for the binary VOC/air mixtures showed fairly good agreement with the experimental results in the case of 1,3-butadiene and propylene. The discrepancies observed for toluene permeation could be minimized by taking into account the effects of the porous support and an influence of the concentration polarization. Finally, the installation of a 0.02 m2 membrane module would reduce 95% of the VOC content introduced at real concentration conditions used in the oil industry.

  17. Volatile Organic Compound (VOC) Removal by Vapor Permeation at Low VOC Concentrations: Laboratory Scale Results and Modeling for Scale Up.

    Science.gov (United States)

    Rebollar-Perez, Georgette; Carretier, Emilie; Lesage, Nicolas; Moulin, Philippe

    2011-03-03

    Petroleum transformation industries have applied membrane processes for solvent and hydrocarbon recovery as an economic alternative to reduce their emissions and reuse evaporated components. Separation of the volatile organic compounds (VOCs) (toluene-propylene-butadiene) from air was performed using a poly dimethyl siloxane (PDMS)/α-alumina membrane. The experimental set-up followed the constant pressure/variable flow set-up and was operated at ~21 °C. The membrane is held in a stainless steel module and has a separation area of 55 × 10-4 m². Feed stream was set to atmospheric pressure and permeate side to vacuum between 3 and 5 mbar. To determine the performance of the module, the removed fraction of VOC was analyzed by Gas Chromatography/Flame Ionization Detector (GC/FID). The separation of the binary, ternary and quaternary hydrocarbon mixtures from air was performed at different flow rates and more especially at low concentrations. The permeate flux, permeance, enrichment factor, separation efficiency and the recovery extent of the membrane were determined as a function of these operating conditions. The permeability coefficients and the permeate flux through the composite PDMS-alumina membrane follow the order given by the Hildebrand parameter: toluene > 1,3-butadiene > propylene. The simulated data for the binary VOC/air mixtures showed fairly good agreement with the experimental results in the case of 1,3-butadiene and propylene. The discrepancies observed for toluene permeation could be minimized by taking into account the effects of the porous support and an influence of the concentration polarization. Finally, the installation of a 0.02 m2 membrane module would reduce 95% of the VOC content introduced at real concentration conditions used in the oil industry.

  18. A rotating differential accelerometer for testing the equivalence principle in space: results from laboratory tests of a ground prototype

    Science.gov (United States)

    Nobili, A. M.; Bramanti, D.; Comandi, G. L.; Toncelli, R.; Polacco, E.

    2003-07-01

    We have proposed to test the equivalence principle (EP) in low Earth orbit with a rapidly rotating differential accelerometer (made of weakly coupled concentric test cylinders) whose rotation provides high frequency signal modulation and avoids severe limitations otherwise due to operation at room temperature [PhRvD 63 (2001) 101101]. Although the accelerometer has been conceived for best performance in absence of weight, we have designed, built and tested a variant of it at 1-g. Here we report the results of measurements performed so far. Losses measured with the full system in operation yield a quality factor only four times smaller than the value required for the proposed high accuracy EP test in space. Unstable whirl motions, which are known to arise in the system and might be a matter of concern, are found to grow as slowly as predicted and can be stabilized. The capacitance differential read-out (the mechanical parts, electronics and software for data analysis) is in all similar to what is needed in the space experiment. In the instrument described here the coupling of the test masses is 24 000 times stiffer than in the one proposed for flight, which makes it 24 000 times less sensitive to differential displacements. With this stiffness it should detect test masses separations of 1.5·10 -2 μm, while so far we have achieved only 1.5 μm, because of large perturbations—due to the motor, the ball bearings, the non-perfect verticality of the system—all of which, however, are absent in space. The effects of these perturbations should be reduced by 100 times in order to perform a better demonstration. Further instrument improvements are underway to fill this gap and also to reduce its stiffness, thus increasing its significance as a prototype of the space experiment.

  19. Liver abnormalities in pregnancy.

    Science.gov (United States)

    Than, Nwe Ni; Neuberger, James

    2013-08-01

    Abnormalities of liver function (notably rise in alkaline phosphatase and fall in serum albumin) are common in normal pregnancy, whereas rise in serum bilirubin and aminotransferase suggest either exacerbation of underlying pre-existing liver disease, liver disease related to pregnancy or liver disease unrelated to pregnancy. Pregnant women appear to have a worse outcome when infected with Hepatitis E virus. Liver diseases associated with pregnancy include abnormalities associated hyperemesis gravidarum, acute fatty liver disease, pre-eclampsia, cholestasis of pregnancy and HELLP syndrome. Prompt investigation and diagnosis is important in ensuring a successful maternal and foetal outcome. In general, prompt delivery is the treatment of choice for acute fatty liver, pre-eclampsia and HELLP syndrome and ursodeoxycholic acid is used for cholestasis of pregnancy although it is not licenced for this indication. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Nitrofurantoin and congenital abnormalities

    DEFF Research Database (Denmark)

    Czeizel, A.E.; Rockenbauer, M.; Sørensen, Henrik Toft;

    2001-01-01

    Objective: To study human teratogenic potential of oral nitrofurantoin treatment during pregnancy. Materials and Methods: Pair analysis of cases with congenital abnormalities and matched population controls in the population-based dataset of the Hungarian Case-Control Surveillance of Congenital...... or fetuses with Down’s syndrome (patient controls), 23 (2.8%) pregnant women were treated with nitrofurantoin. The above differences between population controls and cases may be connected with recall bias, because the case-control pair analysis did not indicate a teratogenic potential of nitrofurantoin use...... during the second and the third months of gestation, i.e. in the critical period for major congenital abnormalities. Conclusion: Treatment with nitrofurantoin during pregnancy does not present detectable teratogenic risk to the fetus....

  1. Comparison of species-level identification and antifungal susceptibility results from diagnostic and reference laboratories for bloodstream Candida surveillance isolates, South Africa, 2009-2010.

    Science.gov (United States)

    Naicker, Serisha D; Govender, Nevashan; Patel, Jaymati; Zietsman, Inge L; Wadula, Jeannette; Coovadia, Yacoob; Kularatne, Ranmini; Seetharam, Sharona; Govender, Nelesh P

    2016-11-01

    From February 2009 through August 2010, we compared species-level identification of bloodstream Candida isolates and susceptibility to fluconazole, voriconazole, and caspofungin between diagnostic and reference South African laboratories during national surveillance for candidemia. Diagnostic laboratories identified isolates to genus/species level and performed antifungal susceptibility testing, as indicated. At a reference laboratory, viable Candida isolates were identified to species-level using automated systems, biochemical tests, or DNA sequencing; broth dilution susceptibility testing was performed. Categorical agreement (CA) was calculated for susceptibility results of isolates with concordant species identification. Overall, 2172 incident cases were detected, 773 (36%) by surveillance audit. The Vitek 2 YST system (bioMérieux Inc, Marcy l'Etoile, France) was used for identification (360/863, 42%) and susceptibility testing (198/473, 42%) of a large proportion of isolates. For the five most common species (n = 1181), species-level identification was identical in the majority of cases (Candida albicans: 98% (507/517); Candida parapsilosis: 92% (450/488); Candida glabrata: 89% (89/100); Candida tropicalis: 91% (49/54), and Candida krusei: 86% (19/22)). However, diagnostic laboratories were significantly less likely to correctly identify Candida species other than C. albicans versus C. albicans (607/664, 91% vs. 507/517, 98%; P Candida species other than C. albicans, under-reported fluconazole resistance for C. parapsilosis and over-reported fluconazole resistance for C. albicans. © The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  2. A new laboratory-based surveillance system (Respiratory DataMart System) for influenza and other respiratory viruses in England: results and experience from 2009 to 2012.

    Science.gov (United States)

    Zhao, H; Green, H; Lackenby, A; Donati, M; Ellis, J; Thompson, C; Bermingham, A; Field, J; Sebastianpillai, P; Zambon, M; Watson, Jm; Pebody, R

    2014-01-23

    During the 2009 influenza A(H1N1) pandemic, a new laboratory-based virological sentinel surveillance system, the Respiratory DataMart System (RDMS), was established in a network of 14 Health Protection Agency (now Public Health England (PHE)) and National Health Service (NHS) laboratories in England. Laboratory results (both positive and negative) were systematically collected from all routinely tested clinical respiratory samples for a range of respiratory viruses including influenza, respiratory syncytial virus (RSV), rhinovirus, parainfluenza virus, adenovirus and human metapneumovirus (hMPV). The RDMS also monitored the occurrence of antiviral resistance of influenza viruses. Data from the RDMS for the 2009–2012 period showed that the 2009 pandemic influenza virus caused three waves of activity with different intensities during the pandemic and post pandemic periods. Peaks in influenza A(H1N1)pdm09 positivity (defined as number of positive samples per total number of samples tested) were seen in summer and autumn in 2009, with slightly higher peak positivity observed in the first post-pandemic season in 2010/2011. The influenza A(H1N1)pdm09 virus strain almost completely disappeared in the second postpandemic season in 2011/2012. The RDMS findings are consistent with other existing community-based virological and clinical surveillance systems. With a large sample size, this new system provides a robust supplementary mechanism, through the collection of routinely available laboratory data at minimum extra cost, to monitor influenza as well as other respiratory virus activity. A near real-time, daily reporting mechanism in the RDMS was established during the London 2012 Olympic and Paralympic Games. Furthermore, this system can be quickly adapted and used to monitor future influenza pandemics and other major outbreaks of respiratory infectious disease, including novel pathogens.

  3. Validating laboratory results in a national observational cohort study without field centers: the Reasons for Geographic and Racial Differences in Stroke cohort.

    Science.gov (United States)

    Gillett, Sarah R; Boyle, Rebekah H; Zakai, Neil A; McClure, Leslie A; Jenny, Nancy S; Cushman, Mary

    2014-11-01

    The REasons for Geographic and Racial Differences in Stroke (REGARDS) study is a prospective cohort of 30,239 Americans in the contiguous United States; the first of this scale to use home visits to obtain, process, and ship biologic samples to a core laboratory. Pre-analytical factors resulting from this study design may affect the results of some laboratory assays. We investigated the impact of REGARDS processing on a variety of analytes. In REGARDS, blood samples were processed in the field by technicians who were trained on standardized methods for phlebotomy and sample processing. Field processing included centrifugation using varying non-uniform equipment and shipping overnight on ice to the University of Vermont, where samples were re-centrifuged for 30,000 ×g-minutes and stored at -80 °C. We assessed the effects of REGARDS sample handling by processing split samples from 20 volunteers using either ideal procedures or simulated REGARDS procedures. Assays for 19 analytes for potential study in REGARDS were then run on both samples and results compared. Spearman correlation coefficients for analytes measured in ideal versus REGARDS processed samples ranged from 0.11 to 1.0. Thirteen of 19 analytes were highly correlated (>0.75), but platelet proteins were more variable. Simulation of non-optimal field processing and shipment to a central laboratory showed high variability in analytes released by platelets. The majority of other analytes produced valid results, but platelet contamination in REGARDS samples makes measurement of platelet proteins unadvisable in these samples. Future analytes considered by REGARDS or similar studies should undergo similar pilot testing. Copyright © 2014. Published by Elsevier Inc.

  4. Abnormal pressures as hydrodynamic phenomena

    Science.gov (United States)

    Neuzil, C.E.

    1995-01-01

    So-called abnormal pressures, subsurface fluid pressures significantly higher or lower than hydrostatic, have excited speculation about their origin since subsurface exploration first encountered them. Two distinct conceptual models for abnormal pressures have gained currency among earth scientists. The static model sees abnormal pressures generally as relict features preserved by a virtual absence of fluid flow over geologic time. The hydrodynamic model instead envisions abnormal pressures as phenomena in which flow usually plays an important role. This paper develops the theoretical framework for abnormal pressures as hydrodynamic phenomena, shows that it explains the manifold occurrences of abnormal pressures, and examines the implications of this approach. -from Author

  5. [Molecular abnormalities in lymphomas].

    Science.gov (United States)

    Delsol, G

    2010-11-01

    Numerous molecular abnormalities have been described in lymphomas. They are of diagnostic and prognostic value and are taken into account for the WHO classification of these tumors. They also shed some light on the underlying molecular mechanisms involved in lymphomas. Overall, four types of molecular abnormalities are involved: mutations, translocations, amplifications and deletions of tumor suppressor genes. Several techniques are available to detect these molecular anomalies: conventional cytogenetic analysis, multicolor FISH, CGH array or gene expression profiling using DNA microarrays. In some lymphomas, genetic abnormalities are responsible for the expression of an abnormal protein (e.g. tyrosine-kinase, transcription factor) detectable by immunohistochemistry. In the present review, molecular abnormalities observed in the most frequent B, T or NK cell lymphomas are discussed. In the broad spectrum of diffuse large B-cell lymphomas microarray analysis shows mostly two subgroups of tumors, one with gene expression signature corresponding to germinal center B-cell-like (GCB: CD10+, BCL6 [B-Cell Lymphoma 6]+, centerine+, MUM1-) and a subgroup expressing an activated B-cell-like signature (ABC: CD10-, BCL6-, centerine-, MUM1+). Among other B-cell lymphomas with well characterized molecular abnormalies are follicular lymphoma (BCL2 deregulation), MALT lymphoma (Mucosa Associated Lymphoid Tissue) [API2-MALT1 (mucosa-associated-lymphoid-tissue-lymphoma-translocation-gene1) fusion protein or deregulation BCL10, MALT1, FOXP1. MALT1 transcription factors], mantle cell lymphoma (cycline D1 [CCND1] overexpression) and Burkitt lymphoma (c-Myc expression). Except for ALK (anaplastic lymphoma kinase)-positive anaplastic large cell lymphoma, well characterized molecular anomalies are rare in lymphomas developed from T or NK cells. Peripheral T cell lymphomas not otherwise specified are a heterogeneous group of tumors with frequent but not recurrent molecular abnormalities

  6. The results of the "essential laboratory tests" applied to new outpatients--re-evaluation of diagnostic efficiencies of the test items.

    Science.gov (United States)

    Takemura, Y; Kobayashi, H; Kugai, N; Sekiguchi, S

    1996-06-01

    We have analyzed diagnostic efficiencies of the individual "Essential laboratory test" items when these tests were applied to 520 new outpatients in the division of comprehensive medicine in a teaching hospital. The integration of these test results with history-taking and physical examination resulted in 544 primary clinical diagnoses which corresponded to the patient's illness complained and in 361 additional diagnoses unrelated to their chief complaints but found by chance by the addition of the test results. Clinical usefulness of these test items were variable depending on the disease category, demonstrating a superior diagnostic efficiency in infectious or inflammatory diseases, liver and biliary tract diseases, hematological disorders or metabolic diseases such as hyperlipidemia and diabetes mellitus, but a lesser degree of usefulness in gastro-intestinal or neurogenic diseases. Urine urobilinogen could not establish its clinical usefulness because of extremely low diagnostic sensitivity even in liver diseases. The leukocyte differential count provided confirmatory information for infectious or inflammatory diseases and was helpful for the estimation of the etiologic nature of infectious diseases. This study failed to terminate a controversy for the adoption of sialic acid instead of erythrocyte sedimentation rate (ESR) in the "Essential laboratory test" items, since the former test showed lower sensitivity, even though higher specificity, in infectious or inflammatory status than ESR. Low albumin globulin ratio (A/G) revealed equivalent diagnostic sensitivity and specificity to the elevated levels in alpha 1 and/or alpha 2 globulin fractions in infectious or inflammatory status, being helpful for the evaluation of patient's general condition at a glance. Incidental analysis for diagnostic values of cholinesterase and random blood glucose for the detection of fatty liver and diabetes mellitus, respectively, suggested that these two tests may be included in

  7. Results of long-term performance and subsequent laboratory tests of RPCs of the L3 forward-backward muon spectrometer

    CERN Document Server

    Alviggi, M G; Carlino, G; Conventi, F; De Asmundis, R; Della Pietra, M; Della Volpe, D; Iengo, P; Paolucci, P; Patricelli, S; Piccolo, D; Sciacca, C; Sehkniadze, G

    2003-01-01

    The RPC chambers in the L3 forward-backward muon spectrometer have been operational from 1994 to the end of LEP running. After dismantling of the L3 detector some of the RPCs modules have been transported to Napoli where their performance has been re-measured with cosmic rays. Results of long-term performance in the LEP environment and of laboratory tests with cosmic rays will be presented. One of the 20 tested chambers, the one with the worst performances, was opened. The chamber was inspected with microscope and also a chemical analysis of the oil coating was performed. We will report in detail about this analysis.

  8. Safety of laboratory analyzers for infection testing - results of the market surveillance by the BfArM until end 2007

    Directory of Open Access Journals (Sweden)

    Siekmeier R

    2009-12-01

    quality control (8. Manufacturers issued corrective measures in 66 cases (90.4% from which 49 and 17 were related to laboratory analyzers and their consumables, respectively. Based on the underlying root causes of product failures these were predominantly customer information (48, recalls (40, software-updates (30 and design changes (9 in the product group of laboratory analyzers as well as customer information (16, recalls (12 and modifications of production and quality management (11 in the group of consumables. The results and experiences obtained since 1999 suggest that the system for post marketing surveillance of IVD is an established tool to ensure product safety, even though the current system can be further enhanced.

  9. Laboratory studies of H2SO4/H2O binary homogeneous nucleation from the SO2+OH reaction: evaluation of the experimental setup and preliminary results

    Directory of Open Access Journals (Sweden)

    M. Kulmala

    2008-08-01

    Full Text Available Binary homogeneous nucleation (BHN of sulphuric acid and water (H2SO4/H2O is one of the most important atmospheric nucleation processes, but laboratory observations of this nucleation process are very limited and there are also large discrepancies between different laboratory studies. The difficulties associated with these experiments include wall loss of H2SO4 and uncertainties in estimation of H2SO4 concentration ([H2SO4] involved in nucleation. We have developed a new laboratory nucleation setup to study H2SO4/H2O BHN kinetics and provide relatively constrained [H2SO4] needed for nucleation. H2SO4 is produced from the SO2+OH→HSO3 reaction and OH radicals are produced from water vapor UV absorption. The residual [H2SO4] were measured at the end of the nucleation reactor with a chemical ionization mass spectrometer (CIMS. Wall loss factors (WLFs of H2SO4 were estimated by assuming that wall loss is diffusion limited and these calculated WLFs were in good agreement with simultaneous measurements of the initial and residual [H2SO4] with two CIMSs. The nucleation zone was estimated from numerical simulations based on the measured aerosol sizes (particle diameter, Dp and [H2SO4]. The measured BHN rates (J ranged from 0.01–220 cm−3 s−1 at the initial and residual [H2SO4] from 108−1010 cm−3, a temperature of 288 K and relative humidity (RH from 11–23%; J increased with increasing [H2SO4] and RH. J also showed a power dependence on [H2SO4] with the exponential power of 3–8. These power dependences are consistent with other laboratory studies under similar [H2SO4] and RH, but different from atmospheric field observations which showed that particle number concentrations are often linearly dependent on [H2SO4]. These results, together with a higher [H2SO4] threshold (108–109 cm−3 needed to produce the unit J measured from the laboratory studies compared to the atmospheric conditions (106–107 cm−3, imply that H2SO4/H2O BHN alone is

  10. Chromosomal abnormalities in patients with sperm disorders

    Directory of Open Access Journals (Sweden)

    L. Y. Pylyp

    2013-02-01

    of sex chromosome aneuploidy and 2 cases of terminal deletion of Y chromosome. Klinefelter syndrome was detected in 67% of patients with azoospermia. A significant increase in the frequency of numerical chromosomal abnormalities was observed in a group of patients with azoospermia (P < 0.001. No differences were detected in the frequency of structural abnormalities in subgroups of patients. An increase in the frequency of chromosomal abnormalities with the decrease of sperm count was observed. Chromosomal abnormalities were detected with frequency 1.1% in a group of patients with normospermia, 1.9% in a group of patients with asthenozoospermia, 4.3% in patients with asthenoteratozoospermia, 6.5% in patients with oligoasthenozoospermia, 11.6% in patients with oligoasthenoteratozoospermia and 35% in a group of patients with azoospermia. Significant increase of the prevalence of chromosomal abnormalities was detected in subgroups of patients with azoospermia (P < 0.001 and oligozoospermia (P = 0.001 as compared to patients with normozoospermia. These results are considered to be criteria for selection of patients in need of cytogenetic studies before in vitro fertilization cycles because of the highest risk of chromosomal abnormalities detection.

  11. Feeling Abnormal: Simulation of Deviancy in Abnormal and Exceptionality Courses.

    Science.gov (United States)

    Fernald, Charles D.

    1980-01-01

    Describes activity in which student in abnormal psychology and psychology of exceptional children classes personally experience being judged abnormal. The experience allows the students to remember relevant research, become sensitized to the feelings of individuals classified as deviant, and use caution in classifying individuals as abnormal.…

  12. [Clinical characteristics of splenic marginal zone lymphoma with abnormal complete blood count].

    Science.gov (United States)

    Yang, Shen-Miao; Jiang, Qian; Jiang, Bin; Chen, Ding-Bao; Wang, Jing; Jiang, Hao; Lu, Jin; Lu, Xi-Jing; Bao, Li; Shi, Hong-Xia; Liu, Yan-Rong; Huang, Xiao-Jun

    2013-02-01

    The aim of this study was to investigate the clinical and laboratorial characteristics of splenic marginal zone lymphoma (SMZL) with an abnormal complete blood count (CBC). Data of 19 newly diagnosed SMZL patients with abnormal CBC were analyzed retrospectively. Seven patients were diagnosed by using splenic histology, 12 patients who did not undergo splenectomy were diagnosed on the basis of typical clinical presentation and cytologic, immunophenotypic and histologic characteristics of peripheral blood and bone marrow, according to SBLG guidelines. The results showed that leukocytosis (≥ 10.0×10(9)/L) was seen in 5 cases (26.3%); leukocytopenia (complete response was achieved in 9 out of 11 (81.8%) patients. It is concluded that SMZL with abnormal CBC has a higher incidence of cytopenia, bone marrow involvement and autoimmune phenomena. Therapeutic strategies consisting of Rituximab show a better efficacy.

  13. Bioassay Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Bioassay Laboratory is an accredited laboratory capable of conducting standardized and innovative environmental testing in the area of aquatic ecotoxicology. The...

  14. HYDROMECHANICS LABORATORY

    Data.gov (United States)

    Federal Laboratory Consortium — Naval Academy Hydromechanics Laboratory The Naval Academy Hydromechanics Laboratory (NAHL) began operations in Rickover Hall in September 1976. The primary purpose...

  15. HYDROMECHANICS LABORATORY

    Data.gov (United States)

    Federal Laboratory Consortium — Naval Academy Hydromechanics LaboratoryThe Naval Academy Hydromechanics Laboratory (NAHL) began operations in Rickover Hall in September 1976. The primary purpose of...

  16. Results of prenatal screening for fetal chromosome abnormality during the first trimester pregnancy in Guangzhou%广州市早孕期产前筛查胎儿染色体异常的结果分析

    Institute of Scientific and Technical Information of China (English)

    许遵鹏; 李蓓; 廖灿; 孙茜; 白雪; 李东至

    2014-01-01

    Objective To evaluate the efficiency of first trimester prenatal screening for fetal chromosome abnormality using maternal serum marker test and/or plus nuchal translucency (NT) in Guangzhou region.Methods The results of prenatal screening were retrospectively analyzed among 43 703 women with singleton pregnancies from January 2007 to September 2012.A total of 43 703 pregnancies between 9 and 13+6 weeks of pregnancy were collected and analyzed for maternal serum pregnancy-associated plasma protein A (PAPPA),free β-human chorionic gonadotropin (free β-hCG) with or without crownrump length (CRL).Nuchal translucency was measured by ultrasonographic scan between 11 and 13+6 weeks of pregnancy.Gestational age was estimated by ultrasonographic scan.The risk values of Down syndrome (DS) and trisomy 18 were calculated using the software Lifcycle.Comparing the difference between the combined screening (PAPPA,free β-hCG and NT) and serum marker screening (PAPPA and free β-hCG).Results Among the 43 703 pregnant women,screening showed that 1385 (3.17%) were Down syndrome positive and 55 (0.13%) were trisomy 18 positive.The final outcomes of pregnancy showed that 142 cases presented chromosomal abnormalities,of which 54 cases suffered from Down syndrome,13 had trisomy 18,and 75 had other chromosome abnormalities.The total detection rate of Down syndrome and trisomy 18 were 83.33% and 76.92%,respectively.The positive rate is lower,and the detection rate is higher in combined screening group than serum marker screening group.The median PAPPA MoM was lower and the median free β-hCG MoM and NT measured value was higher in Down syndrome pregnancies than control group.The median PAPPA and free β-hCG MoM were lower and the median NT measured value was higher in trisomy 18 pregnancies than control group.Conclusion The first trimester prenatal screening can effectively detect Down syndrome and trisomy 18 pregnancy.The combined screening method is superior to the serum

  17. Russia: An Abnormal Country

    Directory of Open Access Journals (Sweden)

    Steven Rosefielde

    2005-06-01

    Full Text Available Andrei Shleifer and Daniel Treisman recently rendered a summary verdict on the post Soviet Russian transition experience finding that the Federation had become a normal country with the west's assistance, and predicting that it would liberalize and develop further like other successful nations of its type. This essay demonstrates that they are mistaken on the first count, and are likely to be wrong on the second too. It shows factually, and on the norms elaborated by Pareto, Arrow and Bergson that Russia is an abnormal political economy unlikely to democratize, westernize or embrace free enterprise any time soon

  18. Abnormal ionization in sonoluminescence

    Science.gov (United States)

    Zhang, Wen-Juan; An, Yu

    2015-04-01

    Sonoluminescence is a complex phenomenon, the mechanism of which remains unclear. The present study reveals that an abnormal ionization process is likely to be present in the sonoluminescing bubble. To fit the experimental data of previous studies, we assume that the ionization energies of the molecules and atoms in the bubble decrease as the gas density increases and that the decrease of the ionization energy reaches about 60%-70% as the bubble flashes, which is difficult to explain by using previous models. Project supported by the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20120002110031) and the National Natural Science Foundation of China (Grant No. 11334005).

  19. Initial performance assessment of the disposal of spent nuclear fuel and high-level waste stored at Idaho National Engineering Laboratory. Volume 1, Methodology and results

    Energy Technology Data Exchange (ETDEWEB)

    Rechard, R.P. [ed.

    1993-12-01

    This performance assessment characterized plausible treatment options conceived by the Idaho National Engineering Laboratory (INEL) for its spent fuel and high-level radioactive waste and then modeled the performance of the resulting waste forms in two hypothetical, deep, geologic repositories: one in bedded salt and the other in granite. The results of the performance assessment are intended to help guide INEL in its study of how to prepare wastes and spent fuel for eventual permanent disposal. This assessment was part of the Waste Management Technology Development Program designed to help the US Department of Energy develop and demonstrate the capability to dispose of its nuclear waste. Although numerous caveats must be placed on the results, the general findings were as follows: Though the waste form behavior depended upon the repository type, all current and proposed waste forms provided acceptable behavior in the salt and granite repositories.

  20. The Past Five Years Conscription Examination Laboratory Test Results Review and Reflection in Our Region%我区近5年征兵体检实验室检验结果的回顾与思考

    Institute of Scientific and Technical Information of China (English)

    陈允

    2014-01-01

    目的:回顾分析广州市越秀区近5年来兵检实验室检查情况,为今后兵检工作提供参考。方法按照国防部征兵体检标准,使用广州军区配发专用试剂,对广州市越秀区市区应征青年进行(抗-HIV)、(HBsAg)、(ALT)、尿常规及吗啡/甲基安非他明(MOR/MAMP)进行严格检测。结果5年内参加征兵体检,其中抗-HIV均为阴性,HBsAg阳性率为2%,ALT异常率为9%,尿常规不合格率为20%,MOR/MAMP阳性率为0.6%。结论广州市越秀区应征男青年 HBsAg携带率较低,ALT及尿常规是实验室检查不合格的主要原因,检验结果的准确性取决于检测全过程,即分析前、分析中、分析后的质量控制。%Objective Retrospective analysis in yuexiu district of guangzhou in recent 5 years inspection laboratory conditions, inspection work to provide the reference for the future. Method According to the department of defense conscription examination standards, the use of guangzhou military area commands issued with special reagent, In yuexiu district of guangzhou city for the youth for human immunodeficiency virus (HIV) antibody (anti - HIV), Hepatitis b virus surface antigen (HBsAg), Alanine aminotransferase (ALT), / methyl amphetamines, routine urine and morphine (MOR/MAMP) for testing, In strict accordance with the reagent instruction to operate. Result Take part in the conscription examination, within five years. The anti - HIV negative, HBsAg positive rate is 2%, ALT abnormal rate was 9%, Routine urine not percent of pass is 20%, MOR/MAMP positive rate was 0.6%. Conclusion Young men in yuexiu district of guangzhou city services HBsAg carry rate is low, ALT and routine urine is a major cause of laboratory examination unqualified, The accuracy of the test results depends on the testing process, Before the analysis, after analysis, analysis of quality control.

  1. Test results of Salmonella sero- and phage typing by the National Reference Laboratories in the Member States of the European Union

    NARCIS (Netherlands)

    Raes M; Ward LR; Maas HME; Wannet WJB; Henken AM; MGB; PHLS/LEP; LIS

    2001-01-01

    The fifth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven) in collaboration with the Public Health Laboratory Services (PHLS, London). All 17 National Reference Laboratories for Salmonella (NRLs-Salmonella) and 1

  2. 2016 Results for Avian Monitoring at the TA-36 Minie Site, TA-39 Point 6, and TA-16 Burn Ground at Los Alamos National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Hathcock, Charles Dean [Los Alamos National Laboratory; Thompson, Brent E. [Los Alamos National Laboratory; Berryhill, Jesse Tobias [Los Alamos National Laboratory

    2017-01-23

    Los Alamos National Security, LLC (LANS) biologists in the Environmental Compliance and Protection Division at Los Alamos National Laboratory (LANL) initiated a multi-year program in 2013 to monitor avifauna at two open detonation sites and one open burn site on LANL property. Monitoring results from these efforts are compared among years and with avifauna monitoring conducted at other areas across LANL. The objectives of this study are to determine whether LANL firing site operations impact bird abundance or diversity. LANS biologists completed the fourth year of this effort in 2016. The overall results from 2016 continue to indicate that operations are not negatively affecting bird populations. Data suggest that community structure may be changing at some sites and this trend will continue to be monitored.

  3. The geochemical reactions resulting from CO{sub 2} injection into the Midale Formation, Weyburn oilfield : a laboratory experimental and modelling study

    Energy Technology Data Exchange (ETDEWEB)

    Azaroual, M.; Durst, P.; Czernichowski-Lauriol, I. [Bureau de Recherches Geologiques et Minieres, Orleans (France); Olsen, D.; Stentoft, N.; Springer, N. [Geological Survey of Denmark and Greenland (Denmark); Rochelle, C.A.; Pearce, J.; Bateman, K.; Birchall, D. [British Geological Survey, Nottingham (United Kingdom)

    2005-07-01

    One aspect of the International Energy Agency's (IEA) Weyburn CO2 Monitoring and Storage Project is the identification of geochemical changes that occur following the injection of carbon dioxide (CO{sub 2}) into the Midale Formation of the Weyburn Field in southern Saskatchewan. This paper summarized the fluid chemical and mineralogical changes noted during laboratory experiments on Midale core from the Weyburn Field. The reactions between injected CO{sub 2}, Midale lithologies and porewaters under temperatures and pressures representative of in-situ conditions were explored. The laboratory studies involved closed system batch experiments reacting samples of Midale reservoir rocks and cap rock with synthetic saline reservoir porewaters. Flow experiments were also performed based on both core flood approaches and column experiments. The duration of experiments ranged from 1 week to 6 months. Changes in fluid chemistry, mineralogy, porosity and permeability were measured. The results were then used to verify predictions from geochemical modelling activities. Experimental and theoretical studies of the effects of CO{sub 2} injection into the Midale lithologies showed variable amounts of carbonate mineral dissolution, some precipitation of gypsum, inhibition of anhydrite dissolution, and slow reactivity of alumino-silicate minerals. These indications helped develop an hypothesis and approaches to simulate the long term evolution of a one dimensional model representing CO{sub 2} injection into the host Midale Marly formation. . 3 refs., 4 figs.

  4. Prevalence and Determinants of True Thyroid Dysfunction Among Pediatric Referrals for Abnormal Thyroid Function Tests.

    Science.gov (United States)

    Lahoti, Amit; Klein, Jason; Schumaker, Tiffany; Vuguin, Patricia; Frank, Graeme

    2016-01-01

    Background/Aims. Abnormalities in thyroid function tests (TFTs) are a common referral reason for pediatric endocrine evaluation. However, a sizable proportion of these laboratory abnormalities do not warrant therapy or endocrine follow-up. The objectives of this study were (a) to evaluate the prevalence of true thyroid dysfunction among pediatric endocrinology referrals for abnormal TFTs; (b) to identify the historical, clinical, and laboratory characteristics that predict decision to treat. Methods. This was a retrospective chart review of patients evaluated in pediatric endocrinology office during a weekly clinic designated for new referrals for abnormal TFTs in 2010. Results. A total of 230 patients were included in the study. Median age at referral was 12 years (range = 2-18); 56% were females. Routine screening was cited as the reason for performing TFTs by 33% patients. Majority was evaluated for hypothyroidism (n = 206). Elevated thyroid-stimulating hormone was the most common referral reason (n = 140). A total of 41 out of 206 patients were treated for hypothyroidism. Conclusions. Prevalence of hypothyroidism was 20%. Thyroid follow-up was not recommended for nearly one third of the patients. Among all the factors analyzed, an elevated thyroid-stimulating hormone level and antithyroglobulin antibodies strongly correlated with the decision to treat (P < .005).

  5. Progress in automated extraction and purification of in situ {sup 14}C from quartz: Results from the Purdue in situ {sup 14}C laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Lifton, Nathaniel, E-mail: nlifton@purdue.edu [Department of Earth, Atmospheric, and Planetary Sciences, Purdue University, 550 Stadium Mall Drive, West Lafayette, IN 47907 (United States); Department of Physics and Astronomy and Purdue Rare Isotope Measurement Laboratory (PRIME Lab), Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907 (United States); Goehring, Brent, E-mail: bgoehrin@tulane.edu [Department of Earth, Atmospheric, and Planetary Sciences, Purdue University, 550 Stadium Mall Drive, West Lafayette, IN 47907 (United States); Wilson, Jim, E-mail: jim.wilson@aeonlaboratories.com [Aeon Laboratories, LLC, 5835 North Genematas Drive, Tucson, AZ 85704 (United States); Kubley, Thomas [Department of Physics and Astronomy and Purdue Rare Isotope Measurement Laboratory (PRIME Lab), Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907 (United States); Caffee, Marc [Department of Earth, Atmospheric, and Planetary Sciences, Purdue University, 550 Stadium Mall Drive, West Lafayette, IN 47907 (United States); Department of Physics and Astronomy and Purdue Rare Isotope Measurement Laboratory (PRIME Lab), Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907 (United States)

    2015-10-15

    Current extraction methods for in situ {sup 14}C from quartz [e.g., Lifton et al., (2001), Pigati et al., (2010), Hippe et al., (2013)] are time-consuming and repetitive, making them an attractive target for automation. We report on the status of in situ {sup 14}C extraction and purification systems originally automated at the University of Arizona that have now been reconstructed and upgraded at the Purdue Rare Isotope Measurement Laboratory (PRIME Lab). The Purdue in situ {sup 14}C laboratory builds on the flow-through extraction system design of Pigati et al. (2010), automating most of the procedure by retrofitting existing valves with external servo-controlled actuators, regulating the pressure of research purity O{sub 2} inside the furnace tube via a PID-based pressure controller in concert with an inlet mass flow controller, and installing an automated liquid N{sub 2} distribution system, all driven by LabView® software. A separate system for cryogenic CO{sub 2} purification, dilution, and splitting is also fully automated, ensuring a highly repeatable process regardless of the operator. We present results from procedural blanks and an intercomparison material (CRONUS-A), as well as results of experiments to increase the amount of material used in extraction, from the standard 5 g to 10 g or above. Results thus far are quite promising with procedural blanks comparable to previous work and significant improvements in reproducibility for CRONUS-A measurements. The latter analyses also demonstrate the feasibility of quantitative extraction of in situ {sup 14}C from sample masses up to 10 g. Our lab is now analyzing unknowns routinely, but lowering overall blank levels is the focus of ongoing research.

  6. Retrospective analysis of 55,769 HbA1c EQA results obtained from professional laboratories and medical offices participating in surveys organized by two European EQA centers over a nine-year period.

    Science.gov (United States)

    Morandi, Pierre-Alain; Deom, André; Kesseler, Dagmar; Cohen, Richard

    2011-01-01

    External Quality Assessment (EQA) is an essential tool for laboratories to monitor the performances of their analyses. It also allows a comparison of methods and types of laboratories (professional laboratories vs. medical offices). We, therefore, compared 55,769 HbA1c EQA results obtained between 1999 and 2008 by laboratories participating in EQA schemes organized by two European centers, Switzerland (center 1) and France (center 2). We used simple, nonparametrical statistics suited to EQA results to calculate the yearly and global precision performances. All the results, including the outliers, were included in the calculations. The best global precision performances were obtained by professional laboratories and medical offices using DCA POCT devices, followed by professional laboratories with the Integra, Hitachi, Cobas Mira, and HPLC groups of devices, and finally by both types of laboratories with the NycoCard POCT devices. When considering yearly precision performances, an overall improvement over time was observed for almost all diagnostic devices of center 1, whereas the trend was less clear for center 2. The HbA1c EQA results collected and analyzed over a 9-year period showed that the DCA POCT devices used either by professional laboratories or medical offices had better reproducibility than laboratory devices (other than POCT) and that a general improvement of yearly precision performances was observed, especially when frequent EQA schemes were organized. © 2011 Wiley-Liss, Inc.

  7. The K-meson form factor and charge radius: linking low-energy data to future high-energy Jefferson Laboratory results

    CERN Document Server

    Krutov, A F; Troitsky, V E

    2016-01-01

    Starting from a successful model of the $\\pi$-meson electromagnetic form factor, we calculate the similar form factor, f_K(Q^2), of the charged K meson for a wide range of the momentum transfer squared, Q^2. The only remaining free parameter is to be determined from the measurements of the K-meson charge radius, r_K. We fit this single parameter to the published data of the NA-7 experiment which measured f_K(Q^2) at Q^2->0 and determine our preferred range of r_K, which happens to be close to recent lattice results. Still, the accuracy in the determination of r_K is poor. However, future measurements of the K-meson electromagnetic form factor at Q^2<~5.5 GeV^2, scheduled in Jefferson Laboratory for 2017, will test our approach and will reduce the uncertainty in r_K significantly.

  8. Test results of Salmonella typing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories - Collaborative study VII on typing of Salmonella

    NARCIS (Netherlands)

    Korver H; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS

    2003-01-01

    Het Communautair Referentie Laboratorium voor Salmonella (CRL-Salmonella, Bilthoven, Nederland) organiseerde in samenwerking met Public Health Laboratory Services (PHLS), London, Verenigd Koninkrijk een zevende ringonderzoek aangaande de typering van Salmonella. Zeventien Nationale Referentie L

  9. Results of the Interlaboratory Exercise CNS/CIEMAT-2008 among Environmental Radioactivity Laboratories (Phosphogypsum); Evaluacion de la Intercomparacion CSN/CIEMAT-2008 entre Laboratorios Nacionales de Radiactividad Ambiental (Fosfoyeso)

    Energy Technology Data Exchange (ETDEWEB)

    Romero, M. L.; Barrera, M.; Valino, F.

    2010-05-27

    The document describes the outcome of the CSN/CIEMAT-2008 interlaboratory test comparison among environmental radioactivity laboratories. The exercise was organised according to the ISO-43 and the ISO/IUPAC/AOAC. Aphosphogypsum material was used as a test sample, in an attempt to evaluate the performance of the laboratories analyzing NORM (Naturally-Occurring Radioactive Materials). The analysis required were: U-238, Th-234, U-234, Th-230, Ra-226, Pb-214, Bi-214, Pb-210, Po-210, Th-232 and U-235, and also gross alpha and gross beta activities. Reference values have been established according to the method of consensus of expert laboratories, with four international laboratories of credited experience: IAEA Seibersdorf, IAEA MEL, IRSN-Orsay and Sta.Teresa ENEA. The results of the exercise were computed for 34 answering laboratories and their analytical performance was assessed using the z-score. Robust statistics of the participants results was applied to obtain the median and standard deviation, to achieve a more complete and objective study of the laboratories performance. The exercise has shown an homogeneous behaviour of laboratories, being statistical parameters from the results close to the assigned Reference Values. Participant laboratories have demonstrated their ability to determine natural radionuclides in phosphogypsum samples (NORM material) with a satisfactory quality level. The scheme has also allowed examining the capability of laboratories to determine the activities of natural radionuclides at the equilibrium. (Author) 10 refs.

  10. Cerro Grande Fire Impact to Water Quality and Stream Flow near Los Alamos National Laboratory: Results of Four Years of Monitoring

    Energy Technology Data Exchange (ETDEWEB)

    B.M. Gallaher; R.J. Koch

    2004-09-15

    In May 2000, the Cerro Grande fire burned about 7400 acres of mixed conifer forest on the Los Alamos National Laboratory (LANL), and much of the 10,000 acres of mountainside draining onto LANL was severely burned. The resulting burned landscapes raised concerns of increased storm runoff and transport of contaminants by runoff in the canyons traversing LANL. The first storms after the fire produced runoff peaks that were more than 200 times greater than prefire levels. Total runoff volume for the year 2000 increased 50% over prefire years, despite a decline in total precipitation of 13% below normal and a general decrease in the number of monsoonal thunderstorms. The majority of runoff in 2000 occurred in the canyons at LANL south of Pueblo Canyon (70%), where the highest runoff volume occurred in Water Canyon and the peak discharge occurred in Pajarito Canyon. This report describes the observed effects of the Cerro Grande fire and related environmental impacts to watersheds at and near Los Alamos National Laboratory (LANL) for the first four runoff seasons after the fire, from 2000 through 2003. Spatial and temporal trends in radiological and chemical constituents that were identified as being associated with the Cerro Grande fire and those that were identified as being associated with historic LANL discharges are evaluated with regard to impacts to the Rio Grande and area reservoirs downstream of LANL. The results of environmental sampling performed by LANL, the New Mexico Environment Department (NMED), and U.S. Geological Survey (USGS) after the Cerro Grande fire are included in the evaluation. Effects are described for storm runoff, baseflow, stream sediments, and area regional reservoir sediment.

  11. The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism.

    Science.gov (United States)

    Demyda-Peyrás, S; Anaya, G; Bugno-Poniewierska, M; Pawlina, K; Membrillo, A; Valera, M; Moreno-Millán, M

    2014-05-01

    Sex chromosome aberrations are known to cause congenital abnormalities and unexplained infertility in horses. Most of these anomalies remain undiagnosed because of the complexity of the horse karyotype and the lack of specialized laboratories that can perform such diagnoses. On the other hand, the utilization of microsatellite markers is a technique widely spread in horse breeding, mostly because of their usage in parentage tests. We studied the usage of a novel combination of diagnostic approaches in the evaluation of a very uncommon case of chromosomal abnormalities in a Spanish purebred colt, primarily detected using a commercial panel of short tandem repeat (STR) makers. Based on these results, we performed a full cytogenetic analysis using conventional and fluorescent in situ hybridization techniques with individual Equus caballus chromosome X and Equus caballus chromosome Y painting probes. We also tested the presence of two genes associated with the sexual development in horses and an extra novel panel of eight microsatellite markers specifically located in the sex chromosome pair. This is the first case report of a leukocyte chimerism between chromosomally normal (64,XY) and abnormal (63,X0) cell lines in horses. Our results indicate that the use of the short tandem repeat markers as a screening technique and as a confirmation utilizing cytogenetic techniques can be used as a very interesting, easy, and nonexpensive diagnostic approach to detect chromosomal abnormalities in the domestic horse.

  12. Would CLSI M53-A have helped in the diagnosis of HIV in Canada? Results of the performance of Canadian laboratories participating in a recent NLHRS proficiency testing panel containing HIV-1 antigen positive (antibody negative) and HIV-2 samples.

    Science.gov (United States)

    Kadivar, K; Malloch, L; Adonsou-Hoyi, Y; Ng, D; Lavoie, S; Pulido, K; Kim, J

    2013-09-01

    The Clinical and Laboratory Standards Institute recently published M53-A, Criteria for Laboratory Testing and Diagnosis of Human Immunodeficiency Virus (HIV) Infection; Approved Guideline (2011), which includes a state of the art algorithm for identifying HIV-1 acute and HIV-2 infections. To assess the ability of Canadian laboratories to detect these sample types and the impact of M53-A, the National Laboratory for HIV Reference Services distributed a special proficiency testing panel. HIVS425-2012Nov22 was sent to 42 laboratories across Canada. It contained one HIV negative sample (B), two HIV-1 positive samples (A and E), one HIV-2 positive sample (C) and one HIV-1/2 antibody negative-HIV-1 antigen positive sample (D). Data was collected and analyzed using DigitalPT; a standardized on-line tool. Forty-one laboratories returned results. Sample B (HIV negative) was identified by 95% of laboratories (39/41) and samples A and E (HIV-1 positive) by 98% (40/41). No laboratory identified sample C as HIV-2 positive, although 85% (35/41) detected reactivity prompting a referral for further testing. The remaining laboratories identified sample C as HIV-1 positive (4), indeterminate (1) or gave no final status (1). Sample D (HIV antibody negative-antigen positive) was correctly identified by two laboratories as HIV-1 antigen positive while 78% (32/41) detected reactivity, recommending further testing. One laboratory did not provide a final status. Alarmingly, six laboratories called this sample HIV negative. Although there is a high quality of HIV testing across Canada, introduction of the M53-A guideline would further improve the ability of laboratories to diagnose HIV-1 acute and HIV-2 infection. Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.

  13. Elevated prothrombin time on routine preoperative laboratory results in a healthy infant undergoing craniosynostosis repair: Diagnosis and perioperative management of congenital factor VII deficiency

    Directory of Open Access Journals (Sweden)

    Kareen L. Jones

    2016-01-01

    Conclusion: A thorough history and physical examination with a high clinical suspicion are vital in preventing hemorrhage during surgeries in children with coagulopathies. Abnormal preoperative lab values should always be confirmed and addressed before proceeding with high-risk surgery. A multidisciplinary discussion is essential to optimize the risk-benefit ratio during the perioperative period.

  14. Combined application of information theory on laboratory results with classification and regression tree analysis: analysis of unnecessary biopsy for prostate cancer.

    Science.gov (United States)

    Hwang, Sang-Hyun; Pyo, Tina; Oh, Heung-Bum; Park, Hyun Jun; Lee, Kwan-Jeh

    2013-01-16

    The probability of a prostate cancer-positive biopsy result varies with PSA concentration. Thus, we applied information theory on classification and regression tree (CART) analysis for decision making predicting the probability of a biopsy result at various PSA concentrations. From 2007 to 2009, prostate biopsies were performed in 664 referred patients in a tertiary hospital. We created 2 CART models based on the information theory: one for moderate uncertainty (PSA concentration: 2.5-10 ng/ml) and the other for high uncertainty (PSA concentration: 10-25 ng/ml). The CART model for moderate uncertainty (n=321) had 3 splits based on PSA density (PSAD), hypoechoic nodules, and age and the other CART for high uncertainty (n=160) had 2 splits based on prostate volume and percent-free PSA. In this validation set, the patients (14.3% and 14.0% for moderate and high uncertainty groups, respectively) could avoid unnecessary biopsies without false-negative results. Using these CART models based on uncertainty information of PSA, the overall reduction in unnecessary prostate biopsies was 14.0-14.3% and CART models were simplified. Using uncertainty of laboratory results from information theoretic approach can provide additional information for decision analysis such as CART. Copyright © 2012 Elsevier B.V. All rights reserved.

  15. Operational comparison of bubble (super heated drop) dosimetry results with routine albedo thermoluminescent dosimetry for a selected group of Pu-238 workers at Los Alamos National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Romero, L.L.; Hoffman, J.M.; Foltyn, E.M.; Buhl, T.E.

    1999-03-01

    This paper is an operational study that compares the use of albedo thermoluminescent dosimeters with bubble dosimeters to determine whether bubble dosimeters do provide a useful daily ALARA tool that can yield measurements close to the dose-of-record. A group of workers at the Los Alamos National Laboratory (LANL) working on the Radioactive Thermoelectric Generators (RTG) for the NASA Cassini space mission wore both bubble dosimeters and albedo dosimeters over a period from 1993 through 1996. The bubble dosimeters were issued and read on a daily basis and the data were used as an ALARA tool. The personnel albedo dosimeter was processed on monthly basis and used as the dose-of-record. The results of this study indicated that cumulative bubble dosimetry results agreed with whole-body albedo dosimetry results within about 37% on average. However it was observed that there is a significant variability of the results on an individual basis both month-to-month and from one individual to another.

  16. Satellite Control Laboratory

    DEFF Research Database (Denmark)

    Wisniewski, Rafal; Bak, Thomas

    2001-01-01

    The Satellite Laboratory at the Department of Control Engineering of Aalborg University (SatLab) is a dynamic motion facility designed for analysis and test of micro spacecraft. A unique feature of the laboratory is that it provides a completely gravity-free environment. A test spacecraft...... of the laboratory is to conduct dynamic tests of the control and attitude determination algorithms during nominal operation and in abnormal conditions. Further it is intended to use SatLab for validation of various algorithms for fault detection, accommodation and supervisory control. Different mission objectives...... can be implemented in the laboratory, e.g. three-axis attitude control, slew manoeuvres, spins stabilization using magnetic actuation and/or reaction wheels. The spacecraft attitude can be determined applying magnetometer measurements...

  17. Laboratory Investigation of Salmonella enterica serovar Poona Outbreak in California: Comparison of Pulsed-Field Gel Electrophoresis (PFGE) and Whole Genome Sequencing (WGS) Results

    Science.gov (United States)

    Kozyreva, Varvara K.; Crandall, John; Sabol, Ashley; Poe, Alyssa; Zhang, Peng; Concepción-Acevedo, Jeniffer; Schroeder, Morgan N.; Wagner, Darlene; Higa, Jeffrey; Trees, Eija; Chaturvedi, Vishnu

    2016-01-01

    Introduction: Recently, Salmonella enterica serovar Poona caused a multistate outbreak, with 245 out of 907 cases occurring in California. We report a comparison of pulsed-field gel electrophoresis (PFGE) results with whole genome sequencing (WGS) for genotyping of Salmonella Poona isolates. Methods: CA Salmonella Poona isolates, collected from July to August 2015, were genotyped by PFGE using XbaI restriction enzyme. WGS was done using Nextera XT library kit with 2x300 bp or 2x250 bp sequencing chemistry on the Illumina MiSeq Sequencer.  Reads were mapped to the de novo assembled serovar Poona draft genome (48 contigs, N50= 223,917) from the outbreak using CLCbio GW 8.0.2. The phylogenetic tree was generated based on hqSNPs calling. Genomes were annotated with CGE and PHAST online tools. In silico MLST was performed using the CGE online tool. Results: Human (14) and cucumber (2) Salmonella Poona isolates exhibited 3 possibly related PFGE patterns (JL6X01.0018 [predominant], JL6X01.0375, JL6X01.0778).  All isolates that were related by PFGE also clustered together according to the WGS. One isolate with a divergent PFGE pattern (JL6X01.0776) served as an outlier in the phylogenetic analysis and substantially differed from the outbreak clade by WGS. All outbreak isolates were assigned to MLST sequence type 447. The majority of the outbreak-related isolates possessed the same set of Salmonella Pathogenicity Islands with few variations. One outbreak isolate was sequenced and analyzed independently by CDC and CDPH laboratories; there was 0 SNP difference in results. Additional two isolates were sequenced by CDC and the raw data was processed through CDPH and CDC analysis pipelines. Both data analysis pipelines also generated concordant results.  Discussion: PFGE and WGS results for the recent CA Salmonella enterica serovar Poona outbreak provided concordant assignment of the isolates to the outbreak cluster. WGS allowed more robust determination of genetic

  18. Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI on typing of Salmonella

    NARCIS (Netherlands)

    Korver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; PHLS-Colindale/London; MGB; LIS

    2002-01-01

    Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing stud

  19. Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and by the EnterNet Laboratories

    NARCIS (Netherlands)

    Korver H; Maas HME; Mooijman KA; Ward LR; Mevius DJ; Wannet WJB; Henken AM; MGB; LIS; PHLS; CIDC

    2003-01-01

    The eighth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS, London, United Kingdom) and the Central Institute for Animal Disease

  20. Autoshaping of abnormal children.

    Science.gov (United States)

    Deckner, C W; Wilcox, L M; Maisto, S A; Blanton, R L

    1980-09-01

    Three experimentally naive abnormal children were exposed to a terminal operant contingency, i.e., reinforcement was delivered only if the children pressed a panel during intervals when it was lighted. Despite the absence of both successive approximation and manual shaping, it was found that each child began to respond discriminatively within a small number of trials. These data replicated previous animal studies concerned with the phenomena of autoshaping and signal-controlled responding. It was also found, however, that one type of autoshaping, the classical conditioning procedure, had a powerful suppressive effect on the discriminative responding. An experimental analysis that consisted procedure, had a powerful suppressive effect on discriminative responding. An experimental analysis that consisted of intrasubject reversal an multiple baseline designs established the internal validity of the findings. The finding of rapid acquisition of signal-controlled responding obtained with the initial procedure is suggessted to have practical significance. The disruptive effects of the classical form of autoshaping are discussed in terms of negative behavioral contrast.

  1. Communication and abnormal behaviour.

    Science.gov (United States)

    Crown, S

    1979-01-01

    In this paper the similarities between normal and abnormal behaviour are emphasized and selected aspects of communication, normal and aberrant, between persons are explored. Communication in a social system may be verbal or non-verbal: one person's actions cause a response in another person. This response may be cognitive, behavioural or physiological. Communication may be approached through the individual, the social situation or social interaction. Psychoanalysis approaches the individual in terms of the coded communications of psychoneurotic symptoms or psychotic behaviour; the humanist-existential approach is concerned more with emotional expression. Both approaches emphasize the development of individual identity. The interaction between persons and their social background is stressed. Relevant are sociological concepts such as illness behaviour, stigma, labelling, institutionalization and compliance. Two approaches to social interactions are considered: the gamesplaying metaphor, e.g. back pain as a psychosocial manipulation--the 'pain game'; and the 'spiral of reciprocal perspectives' which emphasizes the interactional complexities of social perceptions. Communicatory aspects of psychological treatments are noted: learning a particular metaphor such as 'resolution' of the problem (psychotherapy), learning more 'rewarding' behaviour (learning theory) or learning authenticity or self-actualization (humanist-existential).

  2. Abnormally dark or light skin

    Science.gov (United States)

    Hyperpigmentation; Hypopigmentation; Skin - abnormally light or dark ... Normal skin contains cells called melanocytes. These cells produce melanin , the substance that gives skin its color. Skin with ...

  3. An evaluation of the influence of measurement geometry on the uncertainties of photometric model results based on the laboratory measurements of particulate surfaces

    Science.gov (United States)

    Lv, Yunfeng; Sun, Zhongqiu

    2017-01-01

    Sunlight reflected by particulate surfaces carries important information about its physical properties. Modeling the reflectance of different types of particulate samples is an attractive field of study, so estimating the favorable measurement geometry for accurate inversion of photometric model parameters is necessary. This research examines the distribution of the bidirectional reflectance factor (BRF) with different particle sizes by multi-angular reflectance. Two types of particulate samples (one with low reflectance and the other with moderate reflectance) with particle sizes of 0.3, 0.45 and 0.9 mm were measured over a wide viewing range under the assumption of left-to-right symmetry of the BRF. Based on these measurements, we computed the reflectance of particulate surfaces by a photometric model and analyzed the influence of measurement geometry (different combinations of incident zenith angle, viewing zenith angle and azimuth angle) on the inverted parameters and the results modeled by the best-fit parameters. The results show that by using the measurements in the single azimuth (including the principal plane) to invert the model parameters, the difference between the modeled results and measured results will exceed the reflectance change caused by the samples' particle size; this difference is also found when we used the combined measurements at two different incident zenith angles. Including the measurements in the principal plane, an increase in the number of azimuth angles will improve the match between the modeled results and measurements. Our results also confirm that the single-scattering albedo is the only model parameter that could be empirically used to determine the particle sizes of our samples over a wide range of measurement directions. This study proposes several favorable combinations of measurement geometry and also appears to provide a promising empirical reference for the particulate surfaces similar to ours in future laboratory

  4. Preliminary experimental results of Sewage Sludge (SS) Co-digestion with Palm Oil Mill Effluent (POME) for Enhanced Biogas Production in Laboratory Scale Anaerobic Digester

    Science.gov (United States)

    Sivasankari, R.; Kumaran, P.; Normanbhay, Saifuddin; Halim Shamsuddin, Abd

    2013-06-01

    An investigation on the feasibility of co-digesting Sewage Sludge with Palm Oil Mill Effluent for enhancing the biogas production and the corresponding effect of the co-digestion substrate ratio on the biogas production has been evaluated. Anaerobic co-digestion of POME with SS was performed at ratios of 100:0, 70:30, 60:40 and 0:100 to find the optimum blend required for enhanced waste digestion and biogas production. Single stage batch digestion was carried out for 12 days in a laboratory scale anaerobic digester. Co-digestion of sludge's at the 70:30 proportion resulted in optimal COD and C: N ratio which subsequently recorded the highest performance with regards to biogas production at 28.1 L's compared to the 1.98 L's of biogas produced from digestion of SS alone. From the results obtained, it is evident that co-digestion of POME and SS is an attractive option to be explored for enhancement of biogas production in anaerobic digesters.

  5. Photometrics Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Purpose:The Photometrics Laboratory provides the capability to measure, analyze and characterize radiometric and photometric properties of light sources and filters,...

  6. Blackroom Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Enables evaluation and characterization of materials ranging from the ultraviolet to the longwave infrared (LWIR).DESCRIPTION: The Blackroom Laboratory is...

  7. Target Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — [Part of the ATLAS user facility.] The Physics Division operates a target development laboratory that produces targets and foils of various thickness and substrates,...

  8. Target Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — [Part of the ATLAS user facility.] The Physics Division operates a target development laboratory that produces targets and foils of various thickness and substrates,...

  9. Blackroom Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Enables evaluation and characterization of materials ranging from the ultraviolet to the longwave infrared (LWIR). DESCRIPTION: The Blackroom Laboratory is...

  10. Around the laboratories: Dubna: Physics results and progress on bubble chamber techniques; Stanford (SLAC): Operation of a very rapid cycling bubble chamber; Daresbury: Photographs of visitors to the Laboratory; Argonne: Charge exchange injection tests into the ZGS in preparation for a proposed Booster

    CERN Multimedia

    1969-01-01

    Around the laboratories: Dubna: Physics results and progress on bubble chamber techniques; Stanford (SLAC): Operation of a very rapid cycling bubble chamber; Daresbury: Photographs of visitors to the Laboratory; Argonne: Charge exchange injection tests into the ZGS in preparation for a proposed Booster

  11. Virtual Laboratories

    CERN Document Server

    Hut, P

    2006-01-01

    At the frontier of most areas in science, computer simulations play a central role. The traditional division of natural science into experimental and theoretical investigations is now completely outdated. Instead, theory, simulation, and experimentation form three equally essential aspects, each with its own unique flavor and challenges. Yet, education in computational science is still lagging far behind, and the number of text books in this area is minuscule compared to the many text books on theoretical and experimental science. As a result, many researchers still carry out simulations in a haphazard way, without properly setting up the computational equivalent of a well equipped laboratory. The art of creating such a virtual laboratory, while providing proper extensibility and documentation, is still in its infancy. A new approach is described here, Open Knowledge, as an extension of the notion of Open Source software. Besides open source code, manuals, and primers, an open knowledge project provides simul...

  12. Abnormal pressure in hydrocarbon environments

    Science.gov (United States)

    Law, B.E.; Spencer, C.W.

    1998-01-01

    Abnormal pressures, pressures above or below hydrostatic pressures, occur on all continents in a wide range of geological conditions. According to a survey of published literature on abnormal pressures, compaction disequilibrium and hydrocarbon generation are the two most commonly cited causes of abnormally high pressure in petroleum provinces. In young (Tertiary) deltaic sequences, compaction disequilibrium is the dominant cause of abnormal pressure. In older (pre-Tertiary) lithified rocks, hydrocarbon generation, aquathermal expansion, and tectonics are most often cited as the causes of abnormal pressure. The association of abnormal pressures with hydrocarbon accumulations is statistically significant. Within abnormally pressured reservoirs, empirical evidence indicates that the bulk of economically recoverable oil and gas occurs in reservoirs with pressure gradients less than 0.75 psi/ft (17.4 kPa/m) and there is very little production potential from reservoirs that exceed 0.85 psi/ft (19.6 kPa/m). Abnormally pressured rocks are also commonly associated with unconventional gas accumulations where the pressuring phase is gas of either a thermal or microbial origin. In underpressured, thermally mature rocks, the affected reservoirs have most often experienced a significant cooling history and probably evolved from an originally overpressured system.

  13. Systemic abnormalities in liver disease

    Institute of Scientific and Technical Information of China (English)

    Masami Minemura; Kazuto Tajiri; Yukihiro Shimizu

    2009-01-01

    Systemic abnormalities often occur in patients with liver disease. In particular, cardiopulmonary or renal diseases accompanied by advanced liver disease can be serious and may determine the quality of life and prognosis of patients. Therefore, both hepatologists and non-hepatologists should pay attention to such abnormalities in the management of patients with liver diseases.

  14. External Quality Assurance System (EQAS) of the WHO Global Salmonella Surveillance and Laboratory Support Project (Global Salm-Surv) Results from 2001

    DEFF Research Database (Denmark)

    Petersen, A.; Aarestrup, Frank Møller; Evans, M.C.

    An international collaborative study on serotyping and antimicrobial susceptibility testing of eight Salmonella enterica strains was performed to enhance the capacity of national and regional reference laboratories in WHO Global Salm-Surv to conduct Salmonella serotyping and antimicrobial suscept...

  15. Test results of Salmonella sero- and phage typing by the National Reference Laboratories in the Member States of the European Union

    NARCIS (Netherlands)

    Raes M; Ward LR; Maas HME; Wannet WJB; Henken AM; MGB; PHLS/LEP; LIS

    2001-01-01

    Het vijfde Salmonella typerings ringonderzoek is georganiseerd door het Communautair Referentie Laboratorium voor Salmonella (CRL-Salmonella, Bilthoven) in samenwerking met de Public Health Laboratory Services (PHLS, Londen). Alle 17 Nationale Referentie Laboratoria voor Salmonella (NRLs-Salmone

  16. Results of the Interlaboratory Exercise CNS/CIEMAT-05 among Environmental Radioactivity Laboratories (Vegetable Ash); Evaluacion de la Intercomparacion CSN/CIEMAT-2005 entre Laboratorios Nacionales Radiactividad Ambiental (Ceniza Vegetal)

    Energy Technology Data Exchange (ETDEWEB)

    Romero Gonzalez, M. L.; Barrera Izquierdo, M.; Valino Garcia, F.

    2006-07-01

    The document describes the outcome of the CSN/CIEMAT-05 interlaboratory test comparison among environmental radioactivity laboratories. The exercise was organised according to the ISO-43 and the IUPAC {sup I}nternational harmonised protocol for the proficiency testing of analytical chemistry laboratories{sup .} The exercise has been designed to evaluate the capability of national laboratories to determine environmental levels of radionuclides in vegetable ash samples. The sample has been prepared by the Environmental Radiation Laboratory, from the University of Barcelona, and it contains the following radionuclides: Sr-90, Pu-238, Am-241, Th-230, Pb-210, U-238, Ra-226, K-40, Ra-228, TI-208, Cs- 137 and Co-60. Reference values have been established TROUGH the kind collaboration of three international laboratories of recognized experience: IAEA MEL and IRSN-Orsay. The results of the exercise were computed for 35 participating laboratories and their analytical performance was assessed using the z-score approach. Robust statistics of the participant's results was applied to obtain the median and standard deviation, to achieve a more complete and objetiva study of the laboratories' performance. Some difficulties encountered to dissolve the test sample caused a lower response of analyses involving radiochemical separation, thus some laboratories couldn't apply their routine methods and no conclusions on PU-238, Am-241 and Th-230 performances have been obtained. The exercise has revealed an homogeneous behaviour of laboratories, being statistical parameters from the results close to the reference values. The study has shown that participant laboratories perform radioactive determinations in vegetable ash samples with satisfactory quality levels. (Author) 6 refs.

  17. Different chromosome Y abnormalities in a case with short stature

    OpenAIRE

    Balkan, Mahmut; Fidanboy, Mehmet; Özbek, M. Nuri; Alp, M. Nail; Budak, Turgay

    2012-01-01

    We report a case with different chromosome Y abnormalities. Case was an 11-year-old boy, who was diagnosed with short stature, referred to laboratory of human medical genetics laboratory for genetic evaluation. Chromosomal analysis of the case was carried out on peripheral blood lymphocyte culture. Classic cytogenetic analysis (G and C banding) was confirmed by using fluorescence in situ hybridization analysis (FISH) technique. Cytogenetic and FISH analysis showed a mosaic 46,X,i(Yq)/45,X/47,...

  18. Obstructive Sleep Apnea and Lipid Abnormalities

    Science.gov (United States)

    Karkinski, Dimitar; Georgievski, Oliver; Dzekova-Vidimliski, Pavlina; Milenkovic, Tatjana; Dokic, Dejan

    2017-01-01

    BACKGROUND: There has been a great interest in the interaction between obstructive sleep apnea (OSA) and metabolic dysfunction, but there is no consistent data suggesting that OSA is a risk factor for dyslipidemia. AIM: The aim of this cross-sectional study was to evaluate the prevalence of lipid abnormalities in patients suspected of OSA, referred to our sleep laboratory for polysomnography. MATERIAL AND METHODS: Two hundred patients referred to our hospital with suspected OSA, and all of them underwent for standard polysomnography. All patients with respiratory disturbance index (RDI) above 15 were diagnosed with OSA. In the morning after 12 hours fasting, the blood sample was collected from all patients. Blood levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL), were determined in all study patients. In the study, both OSA positive and OSA negative patients were divided according to the body mass index (BMI) in two groups. The first group with BMI ≤ 30 kg/m^2 and the second group with BMI > 30 kg/m^2. RESULTS: OSA positive patients with BMI ≤ 30 kg/m^2 had statistically significant higher levels of triglycerides and total cholesterol, and statistically significant lower level of HDL compared to OSA negative patients with BMI ≤ 30. There were no statistically significant differences in age and LDL levels between these groups. OSA positive patients with BMI > 30 kg/m^2 had higher levels of triglycerides, total cholesterol and LDL and lower levels of HDL versus OSA negative patients with BMI > 30 kg/m^2, but without statistically significant differences. CONCLUSION: OSA and obesity are potent risk factors for dyslipidemias. OSA could play a significant role in worsening of lipid metabolism in non-obese patients. But in obese patients, the extra weight makes the metabolic changes of lipid metabolism, and the role of OSA is not that very important like in non-obese patients. PMID

  19. Applicability and robustness of the hen's egg test for analysis of micronucleus induction (HET-MN): results from an inter-laboratory trial.

    Science.gov (United States)

    Greywe, Daniela; Kreutz, Jürgen; Banduhn, Norbert; Krauledat, Matthias; Scheel, Julia; Schroeder, Klaus R; Wolf, Thorsten; Reisinger, Kerstin

    2012-08-30

    The hen's egg test for analysis of micronucleus formation (HET-MN) was developed several years ago to provide an alternative test system to the in vivo micronucleus test. In order to assess its applicability and robustness, a study was carried out at the University of Osnabrueck (lab A) and at the laboratories of Henkel AG & Co. KGaA (lab B). Following transfer of the method to lab B, a range of test substances that had been pre-tested at lab A, were tested at Henkel: the genotoxins cyclophosphamide, dimethylbenz(a)anthracene, methotrexate, acrylamide, azorubin, N-nitroso-dimethylamine and the non-genotoxins, orange G and isopropyl myristate. In a second phase, additional compounds with known in vivo properties were examined in both labs: the non-genotoxin, ampicillin, the "irrelevant" positives, isophorone and 2,4-dichlorophenol ("irrelevant" means positive in standard in vitro tests, but negative in vivo), the clastogen p-chloroaniline, and the aneugens carbendazim and vinorelbine. All substances were correctly predicted in both labs with respect to their in vivo genotoxic properties, indicating that the HET-MN may have an improved predictivity compared with current standard in vitro test systems. The results support the promising role of the HET-MN assay as a supplement to existing test batteries.

  20. Prevalence and Genotype Distribution of HPV Infection in China: Analysis of 51,345 HPV Genotyping Results from China's Largest CAP Certified Laboratory.

    Science.gov (United States)

    Zeng, Zhengyu; Yang, Huaitao; Li, Zaibo; He, Xuekui; Griffith, Christopher C; Chen, Xiamen; Guo, Xiaolei; Zheng, Baowen; Wu, Shangwei; Zhao, Chengquan

    2016-01-01

    The prevalence of cervical Human Papillomavirus (HPV) infection varies greatly worldwide and data regarding HPV prevalence and genotypes in China are limited. HPV testing results were retrospectively examined at KingMed Diagnostics, the largest independent pathology laboratory in China, from January 2011 to June 2014. All testing was performed using the 26 HPV Genotyping Panel of Tellgenplex (TM) xMAP™ HPV DNA Test assay (TELLGEN, Shanghai, China). Overall prevalence, age-specific prevalence and genotype distributions were analyzed. A total of 51,345 samples were tested and the overall HPV prevalence was 26%, with 21.12% positive for high risk (HR) HPV and 8.37% positive for low risk HPV. 80% of HPV positive cases were positive for a single HPV type. The three most common HR HPV types detected were HPV-52, -16, and -58, in descending order. HPV-18 was only the 6(th) most common type. When women were divided into three age groups: HR HPV had the highest prevalence rate in women HR HPV genotypes also varied among these three age groups. To the best of our knowledge, this is largest routine clinical practice report of HPV prevalence and genotypes in a population of women having limited cervical cancer screening. HPV-52 was the most prevalent HR HPV type in this population of women followed by HPV-16 and HPV-58. The overall and age-specific prevalence and genotype distribution of HR HPV are different in this Chinese population compared to that reported from Western countries.

  1. A study on the uncertainty in the analytical results of uranium potentiometric titration method used by the Safeguards Laboratory of CNEN - Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Barros, Pedro Dionisio de; Araujo, Radier Mario S. de [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil). Coordenacao de Salvaguardas e Protecao Fisica. Lab. de Salvaguardas], E-mail: pedrodio@ird.gov.br, E-mail: radier@ird.gov.br; Dunley, Leonardo Souza [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil). Coordenacao de Salvaguardas e Protecao Fisica], E-mail: leonardo@cnen.gov.br

    2007-07-01

    Independent determination of uranium content in nuclear materials to assess operator's declarations is an important tool in the safeguards approaches applied to nuclear installations. This determination may be performed by nondestructive methods, generally done in the field using portable radiation detection systems, or destructive methods by chemical analysis when more accurate results are necessary. In Brazil, samples are taken during safeguards inspections and sent to Safeguards Laboratory-LASAL of the Brazilian Nuclear Energy Commission-CNEN, where analysis is performed. The method used by LASAL for determination of uranium in several forms of nuclear material is the Davies and Gray/NBL using a potentiometric titrator, which performs the titration of uranium IV by a standard solution of K{sub 2}Cr{sub 2}O{sub 7}. Except the titration itself, which is performed by the addition of a certain volume of K{sub 2}Cr{sub 2}O{sub 7} solution, all the proceedings for sample and standard solutions preparation are conducted in weigh basis, including the aliquanting process. Firstly, all the parameters that can interfere with the uncertainty were studied. In order to evaluate their contribution in the final uncertainty, the uncertainties of all steps of the analysis method were estimated and compiled. Sensitivity coefficients were calculated as necessary for the appropriate propagation of uncertainties. Therefore, the standard and the expanded uncertainty were obtained. The confidence interval used was 95.45% with a coverage factor (k) of about 2. (author)

  2. Microbiology of shallow subsurface aquifer and carbonate rocks studied by culture-dependent and culture-independent methods. Preliminary results on an underground laboratory, the LSBB, Rustrel, France

    Directory of Open Access Journals (Sweden)

    Galès Grégoire

    2016-01-01

    Full Text Available We investigated the microbiology of a shallow subsurface site, the LSBB, located near Avignon. This site lies in carbonate rocks, belonging to the Urgonian facies. Rock, concrete and water samples were collected and directly transferred to the laboratory. Studies of microorganisms as pure cultures are the only way to get their real physiological properties. Nevertheless, microbiologists cannot cultivate and isolate the majority of microorganisms for several reasons, one being our lack of understanding of their minimal needs. Molecular studies, e.g. extraction and sequencing of the total nucleic acids present in an environment provide phylogenetic and metabolic information on uncultivated microorganisms. We performed aerobic and anaerobic culture with various electron acceptors and donors, searching for heterotrophic, methanogenic, sulphate- nitrate- and FeIII- reducing Prokaryotes. We also performed DNA extractions and PCR amplification of ribosomal RNA genes, to test if our protocols were adapted to this environment. Our results show that the LSBB galleries are colonized by a low diversity microbiote, with a strong influence of anthropogenic activities. Further studies will link the microorganisms biodiversity and the petrophysic properties of rocks.

  3. Assessing effects of the e-Chasqui laboratory information system on accuracy and timeliness of bacteriology results in the Peruvian tuberculosis program.

    Science.gov (United States)

    Blaya, Joaquin A; Shin, Sonya S; Yagui, Martin J A; Yale, Gloria; Suarez, Carmen; Asencios, Luis; Fraser, Hamish

    2007-10-11

    We created a web-based laboratory information system, e-Chasqui to connect public laboratories to health centers to improve communication and analysis. After one year, we performed a pre and post assessment of communication delays and found that e-Chasqui maintained the average delay but eliminated delays of over 60 days. Adding digital verification maintained the average delay, but should increase accuracy. We are currently performing a randomized evaluation of the impacts of e-Chasqui.

  4. LabPush: a pilot study of providing remote clinics with laboratory results via short message service (SMS) in Swaziland, Africa - a qualitative study.

    Science.gov (United States)

    Hao, Wen-Rui; Hsu, Yi-Hsin; Chen, Kuan-Chen; Li, Hsien-Chang; Iqbal, Usman; Nguyen, Phung-Anh; Huang, Chih-Wei; Yang, Hsuan-Chia; Lee, Peisan; Li, Mei-Hsuan; Hlatshwayo, Sharoon Lungile; Li, Yu-Chuan Jack; Jian, Wen-Shan

    2015-01-01

    Developing countries are confronting a steady growth in the prevalence of the infectious diseases. Mobile technologies are widely available and can play an important role in health care at the regional, community, and individual levels. Although labs usually able to accomplish the requested blood test and produce the results within two days after receiving the samples, but the time for the results to be delivered back to clinics is quite variable depending on how often the motorbike transport makes trips between the clinic and the lab. In this study, we seek to assess factors facilitating as well as factors hindering the adoption of mobile devices in the Swazi healthcare through evaluating the end-users of the LabPush system. A qualitative study with semi-structured and in-depth one on one interviews were conducted over two month period July-August 2012. Purposive sampling was used; participants were those operating and using the LabPush system at the remote clinics, at the national laboratory and the supervisors of users at Swaziland. Interview questions were focused on perceived of ease of use and usefulness of the system. All interviews were recorded and then transcribed. This study had aimed its primary focus on reducing TAT, prompt patient care, reducing bouncing of patients and defaulting of patients which were challenges that the clinicians have always had. Therefore, the results revealed several barriers and facilitators to the adoption of mobile device by healthcare providers in the Swaziland. The themes Shortens TAT, Technical support, Patient-centered care, Mindset, Improved communication, Missing Reports, Workload, Workflow, Security of smart phone, Human error and Ownership are sorted by facilitators to barriers. Thus the end-users perspective, prompt patient care, reduced bouncing of patients, technical support, better communication, willing participant and social influence were facilitators of the adoption m-health in the Swazi healthcare. Copyright

  5. OPHTHALMOLOGIC ABNORMALITIES IN CHILDREN WITH IMPAIRED HEARING

    Directory of Open Access Journals (Sweden)

    Inderjit

    2014-02-01

    Full Text Available AIM: To determine the nature of ophthalmologic abnormalities in severe and profound grades of hearing impaired children and to treat visual impairment if any at the earliest . MATERIAL AND METHODS: Study was conducted on100 children in the age group of 5 - 14 years with severe and profound hearing loss visiting outpatient department of Ram Lal Eye and ENT hospital Govt. Medical College Amritsar and subjected to detailed ophthalmological examination. RESULTS: 100 children in the age group 5 - 14 years with hearing impairment were enrolled for t he study , 68 had profound and 32 had severe hearing loss . Visual disorders were found to be as high as 71%. Highest percentage was seen in children aged 7 years. Majority of them (50% had refractive error. Out of these 50 children , 28(56% had myopia , 10 (20% hypermetropia and 12(24% had astigmatism . The other ophthalmic abnormalities in our study were conjunctivitis 14(19.71% , fundus abnormalities and squint 11(15.49% , blepharitis 5 (7.04% , vitamin A deficiency 6 (8.04% , amblyopia 8 (11.26% , pupil disorder 3 (4.22% , cataract 3 (4.22% and heterochromia iridis 7 (9.85%. CONCLUSION : The high prevalence of ophthalmic abnormalities in deaf children mandate screening them for possible ophthalmic abnormalities. Early diagnosis and correction of visual d isturbances would go a long way in social and professional performance of these children.

  6. Do Laboratory Results Concerning High-Viscosity Glass-Ionomers versus Amalgam for Tooth Restorations Indicate Similar Effect Direction and Magnitude than that of Controlled Clinical Trials? - A Meta-Epidemiological Study.

    Directory of Open Access Journals (Sweden)

    Steffen Mickenautsch

    Full Text Available A large percentage of evidence concerning dental interventions is based on laboratory research. The apparent wealth of laboratory evidence is sometimes used as basis for clinical inference and recommendations for daily dental practice. In this study two null-hypotheses are tested: whether trial results from laboratory and controlled clinical trials concerning the comparison of high-viscosity glass-ionomer cements (HVGIC to amalgam for restorations placed in permanent posterior teeth have: (i similar effect direction and (ii similar effect magnitude.7 electronic databases were searched, as well as reference lists. Odds ratios (OR and Standardised Mean Differences (SMD with 95% Confidence intervals were computed for extracted dichotomous and continuous data, respectively. Pooled effect estimates for laboratory and clinical data were computed to test for effect direction. Odds ratios were converted into SMDs. SMDs from laboratory and clinical data were statistically compared to test for differences in effect magnitude. The analysed results were further investigated within the context of potential influencing or confounding factors using a Directed acyclic graph.Of the accepted eight laboratory and nine clinical trials, 13 and 21 datasets could be extracted, respectively. The pooled results of the laboratory datasets were highly statistically significant in favor of amalgam. No statistically significant differences, between HVGICs and amalgam, were identified for clinical data. For effect magnitude, statistically significant differences between clinical and laboratory trial results were found. Both null-hypotheses were rejected.Laboratory results concerning high-viscosity glass-ionomers versus amalgam for tooth restorations do not indicate similar effect direction and magnitude than that of controlled clinical trials.

  7. Abnormal fetal head shape: aetiology and management

    DEFF Research Database (Denmark)

    Petersen, Olav Bjørn; David, Anna; Thomasson, Louise

    2007-01-01

    Background: Abnormal head shape is an uncommon finding on prenatal ultrasound, often associated with breech presentation, spinabifida, aneuploidy or secondary to oligohydramnios or fetal position. Other aetiologies are rarer and may be more difficult to define. Objective: To determine the aetiolo...... incidence of genetic syndromes, in the absence of a clear diagnosis, referral to a tertiary centre and genetic input is advised as detection of subtle sonographic features may aid diagnosis, allowing for targeted molecular analysis. An algorithm for management will be proposed....... and define management pathways for fetuses with an abnormal skull shape. Methods: Our FMU databases were searched to ascertain all fetuses with an abnormal skull shape. Sonographic findings, diagnosis and outcome were reviewed. Results: Of the 370 cases identified, 31.6% were associated with spinabifida...

  8. Persistent Pain and Sensory Abnormalities after Abdominoplasty

    DEFF Research Database (Denmark)

    Presman, Benjamin; Finnerup, Kenneth; Andresen, Sven Robert

    2015-01-01

    University Hospital, Denmark. The questionnaire included questions about pain and sensory abnormalities located to the abdominal skin, and physical and psychological function; patient satisfaction with surgery was rated on a 4-point scale. RESULTS: One hundred seventy patients answered the questionnaire......%) patients. The majority of patients reported improvement on all physical and psychological factors. Patients with pain were more often disappointed with the surgery and unwilling to recommend the surgery. CONCLUSIONS: Overall, patients were satisfied with the procedure, although abnormal abdominal skin....... Fourteen patients (8.2%) reported pain within the past 7 days related to the abdominoplasty. Abnormal abdominal skin sensation was common and reported by 138 patients (81%). Sensory hypersensitivity was associated with the presence of persistent pain. Satisfaction with the procedure was reported by 149 (88...

  9. Temporal abnormalities in children with developmental dyscalculia.

    Science.gov (United States)

    Vicario, Carmelo Mario; Rappo, Gaetano; Pepi, Annamaria; Pavan, Andrea; Martino, Davide

    2012-01-01

    Recent imaging studies have associated Developmental dyscalculia (DD) to structural and functional alterations corresponding Parietal and the Prefrontal cortex (PFC). Since these areas were shown also to be involved in timing abilities, we hypothesized that time processing is abnormal in DD. We compared time processing abilities between 10 children with pure DD (8 years old) and 11 age-matched healthy children. Results show that the DD group underestimated duration of a sub-second scale when asked to perform a time comparison task. The timing abnormality observed in our DD participants is consistent with evidence of a shared fronto-parietal neural network for representing time and quantity.

  10. Declining total leucoyte count, an indication of transient abnormal myelopoisis

    Directory of Open Access Journals (Sweden)

    Yogesh K Yadav

    2012-01-01

    Full Text Available Neonates with Down syndrome have an increased predisposition to transient abnormal myelopoisis, a haematological abnormality which is characterized by uncontrolled proliferation of myeloblasts. The unique ability of this disorder to spontaneously enter in to a state of remission led to the creation of a new class in 2008 World Health Organization (W.H.O. classification of haemopoitic and lymphoid tumours. It has now been classified as transient abnormal myelopoisis (TAM. The cause of remission is still not clear although it is possibly linked to the abnormal expression of GATA-1 transcription factor and to a switch from hepatic haematopoesis to medullary haematopoesis. Simple laboratory monitoring by serial complete blood count and peripheral smear can give indication of this rare disorder. As very few cases of this disorder exist we share our experience and report a case of transient myeloproliferative disorder in a male newborn having Down syndrome.

  11. Satellite Control Laboratory

    DEFF Research Database (Denmark)

    Wisniewski, Rafal; Bak, Thomas

    2001-01-01

    The Satellite Laboratory at the Department of Control Engineering of Aalborg University (SatLab) is a dynamic motion facility designed for analysis and test of micro spacecraft. A unique feature of the laboratory is that it provides a completely gravity-free environment. A test spacecraft...... is suspended on an air bearing, and rotates freely in 3 degrees of freedom. In order to avoid any influence of the gravitational force the centre of mass of the satellite is placed in the geometric centre of the air bearing by an automatic balancing system. The test spacecraft is equipped with a three...... of the laboratory is to conduct dynamic tests of the control and attitude determination algorithms during nominal operation and in abnormal conditions. Further it is intended to use SatLab for validation of various algorithms for fault detection, accommodation and supervisory control. Different mission objectives...

  12. Abnormal traffic flow data detection based on wavelet analysis

    Directory of Open Access Journals (Sweden)

    Xiao Qian

    2016-01-01

    Full Text Available In view of the traffic flow data of non-stationary, the abnormal data detection is difficult.proposed basing on the wavelet analysis and least squares method of abnormal traffic flow data detection in this paper.First using wavelet analysis to make the traffic flow data of high frequency and low frequency component and separation, and then, combined with least square method to find abnormal points in the reconstructed signal data.Wavelet analysis and least square method, the simulation results show that using wavelet analysis of abnormal traffic flow data detection, effectively reduce the detection results of misjudgment rate and false negative rate.

  13. Performance of the CellaVision ® DM96 system for detecting red blood cell morphologic abnormalities

    Directory of Open Access Journals (Sweden)

    Christopher L Horn

    2015-01-01

    Full Text Available Background: Red blood cell (RBC analysis is a key feature in the evaluation of hematological disorders. The gold standard light microscopy technique has high sensitivity, but is a relativity time-consuming and labor intensive procedure. This study tested the sensitivity and specificity of gold standard light microscopy manual differential to the CellaVision ® DM96 (CCS; CellaVision, Lund, Sweden automated image analysis system, which takes digital images of samples at high magnification and compares these images with an artificial neural network based on a database of cells and preclassified according to RBC morphology. Methods: In this study, 212 abnormal peripheral blood smears within the Calgary Laboratory Services network of hospital laboratories were selected and assessed for 15 different RBC morphologic abnormalities by manual microscopy. The same samples were reassessed as a manual addition from the instrument screen using the CellaVision ® DM96 system with 8 microscope high power fields (×100 objective and a 22 mm ocular. The results of the investigation were then used to calculate the sensitivity and specificity of the CellaVision ® DM96 system in reference to light microscopy. Results: The sensitivity ranged from a low of 33% (RBC agglutination to a high of 100% (sickle cells, stomatocytes. The remainder of the RBC abnormalities tested somewhere between these two extremes. The specificity ranged from 84% (schistocytes to 99.5% (sickle cells, stomatocytes. Conclusions: Our results showed generally high specificities but variable sensitivities for RBC morphologic abnormalities.

  14. Neurobehavioural Correlates of Abnormal Repetitive Behaviour

    Directory of Open Access Journals (Sweden)

    R. A. Ford

    1991-01-01

    Full Text Available Conditions in which echolalia and echopraxia occur are reviewed, followed by an attempt to elicit possible mechanisms of these phenomena. A brief description of stereotypical and perseverative behaviour and obsessional phenomena is given. It is suggested that abnormal repetitive behaviour may occur partly as a result of central dopaminergic dysfunction.

  15. Imaging findings of sternal abnormalities

    Energy Technology Data Exchange (ETDEWEB)

    Franquet, T. [Dept. of Radiology, Hospital de Sant Pau, Universidad Autonoma de Barcelona (Spain); Gimenez, A. [Dept. of Radiology, Hospital de Sant Pau, Universidad Autonoma de Barcelona (Spain); Alegret, X. [Dept. of Radiology, Hospital de Sant Pau, Universidad Autonoma de Barcelona (Spain); Sanchis, E. [Dept. of Radiology, Hospital de Sant Pau, Universidad Autonoma de Barcelona (Spain); Rivas, A. [Dept. of Radiology, Hospital Vall d`Hebron, Universidad Autonoma de Barcelona (Spain)

    1997-05-01

    Radiographic findings in the sternal abnormalities are often nonspecific, showing appearances from a localized benign lesion to an aggressive lesion as seen with infections and malignant neoplasms. A specific diagnosis of sternal abnormalities can be suggested on the basis of CT and MR characteristics. Familiarity with the presentation and variable appearance of sternal abnormalities may aid the radiologist is suggesting a specific diagnosis. We present among others characteristic radiographic findings of hemangioma, chondrosarcoma, hydatid disease, and SAPHO syndrome. In those cases in which findings are not specific, cross-sectional imaging modalities may help the clinician in their management. (orig.)

  16. The identification and description of critical thinking behaviors in the practice of clinical laboratory science, Part 1: Design, implementation, evaluation, and results of a national survey.

    Science.gov (United States)

    Kenimer, Elizabeth A

    2002-01-01

    The ability to think critically has been identified as a pivotal attribute of health care professionals. The purpose of this study was to identify and describe critical thinking (CT) behaviors and observable events resulting from CT, important in the practice of clinical laboratory science. Medical, educational, and psychological literature related to the many behaviors characterized under the CT rubric has been focused by an operationalized definition positing CT to be a metaprocess that facilitates learning by interlinking the more basic processes associated with different learning orientations: behaviorist, cognitivist, humanist, and situated/contextual learning. A total of 65 CT behaviors were identified through a process including discovery (expert interviews, a focus group with practitioners) and refinement (literature comparisons, expert rankings/reviews, pilot survey). CT behaviors were characterized further through a national survey of practitioners (n = 1,571, > 50% of time in bench work). Using a 6-point scale, practitioners ranked the importance of the CT behaviors. Important CT behaviors were characterized as being not only cognitive in nature, but also behavioral, affective, and situated/contextual. These findings suggest a stronger relationship between CT behaviors and all aspects of practice than previously reported. Given that these behaviors span all learning domains, findings support the metaprocess view of CT. Implications are that CT behaviors cannot be learned or taught, apart from a discipline-related practice setting. This study contributes to learning theory related to transfer and how learning occurs and to literature describing survey methodology. It also provides a basis for measurement of CT in practice settings.

  17. Molecular characterization of a dechlorinating community resulting from in situ biostimulation in a trichloroethene-contaminated deep, fractured basalt aquifer and comparison to a derivative laboratory culture.

    Science.gov (United States)

    Macbeth, Tamzen W; Cummings, David E; Spring, Stefan; Petzke, Lynn M; Sorenson, Kent S

    2004-12-01

    Sodium lactate additions to a trichloroethene (TCE) residual source area in deep, fractured basalt at a U.S. Department of Energy site have resulted in the enrichment of the indigenous microbial community, the complete dechlorination of nearly all aqueous-phase TCE to ethene, and the continued depletion of the residual source since 1999. The bacterial and archaeal consortia in groundwater obtained from the residual source were assessed by using PCR-amplified 16S rRNA genes. A clone library of bacterial amplicons was predominated by those from members of the class Clostridia (57 of 93 clones), of which a phylotype most similar to that of the homoacetogen Acetobacterium sp. strain HAAP-1 was most abundant (32 of 93 clones). The remaining Bacteria consisted of phylotypes affiliated with Sphingobacteria, Bacteroides, Spirochaetes, Mollicutes, and Proteobacteria and candidate divisions OP11 and OP3. The two proteobacterial phylotypes were most similar to those of the known dechlorinators Trichlorobacter thiogenes and Sulfurospirillum multivorans. Although not represented by the bacterial clones generated with broad-specificity bacterial primers, a Dehalococcoides-like phylotype was identified with genus-specific primers. Only four distinct phylotypes were detected in the groundwater archaeal library, including predominantly a clone affiliated with the strictly acetoclastic methanogen Methanosaeta concilii (24 of 43 clones). A mixed culture that completely dechlorinates TCE to ethene was enriched from this groundwater, and both communities were characterized by terminal restriction fragment length polymorphism (T-RFLP). According to T-RFLP, the laboratory enrichment community was less diverse overall than the groundwater community, with 22 unique phylotypes as opposed to 43 and a higher percentage of Clostridia, including the Acetobacterium population. Bioreactor archaeal structure was very similar to that of the groundwater community, suggesting that methane is

  18. Abnormal Bleeding During Menopause Hormone Therapy: Insights for Clinical Management

    OpenAIRE

    2013-01-01

    Objective Our objective was to review the involved mechanisms and propose actions for controlling/treating abnormal uterine bleeding during climacteric hormone therapy. Methods A systemic search of the databases SciELO, MEDLINE, and Pubmed was performed for identifying relevant publications on normal endometrial bleeding, abnormal uterine bleeding, and hormone therapy bleeding. Results Before starting hormone therapy, it is essential to exclude any abnormal organic condition, identify women a...

  19. Conversion of results from ash leaching in laboratory to field conditions. Final report; Oeversaettning av resultat fraan asklaknings foersoek i laboratorium till faeltfoerhaallanden. Slutrapport

    Energy Technology Data Exchange (ETDEWEB)

    Westling, Olle; Larsson, Per-Erik [Swedish Environmental Research Inst., Goeteborg (Sweden)

    2004-09-01

    The leaching of different elements from stabilised wood ash used for recycling of nutrients to forest soil was investigated and compared in both laboratory and field experiments. The aim was to evaluate if a simplified laboratory method can be used to describe the leaching in field conditions in a realistic way. The laboratory leaching study was performed by mixing wood ash and distilled water, adjusted to pH 4.2. The leachate was poured off after 24 h and new solution added. The leachate was analysed for pH, acid neutralising capacity and base cations. This procedure, with daily change of solution, continued for 30 days, simulating the time of a forest rotation (80-100 years). The field study used wood ash in litter bags placed under the humus layer in a spruce forest with and without contact with mycorrhiza. Litterbags were removed and analysed after 6, 12, 18 and 30 months. The leaching of wood ash and crushed limestone were also compared in both the laboratory and the field study. The weight loss of wood ash and limestone after 30 days in the laboratory study (simulating a forest rotation) varied between 20 and 35%, indicating a very long time for complete weathering. The weight loss in the field study after 30 months (8-22%) also indicated a long weathering time. The leaching of wood ash during the 30-month field experiment showed a variation in the leaching pattern for different elements. The leaching of calcium increased in the beginning of the study, followed by a decrease. The leaching of magnesium was relatively linear in contrast to potassium, with a fast leaching of 35 to 40% of the original content after 6 months. The experiment with leaching of wood ash and crushed limestone during field conditions with and without contact with mycorrhiza showed no significant differences between the treatments. The comparison between laboratory and field leaching generally showed that the different methods describe the leaching rate of base cations in a similar way

  20. The Development of the Chemin Mineralogy Instrument and Its Deployment on Mars (and Latest Results from the Mars Science Laboratory Rover Curiosity)

    Science.gov (United States)

    Blake, David F.

    2014-01-01

    The CheMin instrument (short for "Chemistry and Mineralogy") on the Mars Science Laboratory rover Curiosity is one of two "laboratory quality" instruments on board the Curiosity rover that is exploring Gale crater, Mars. CheMin is an X-ray diffractometer that has for the first time returned definitive and fully quantitative mineral identifications of Mars soil and drilled rock. I will describe CheMin's 23-year development from an idea to a spacecraft qualified instrument, and report on some of the discoveries that Curiosity has made since its entry, descent and landing on Aug. 6, 2012, including the discovery and characterization of the first habitable environment on Mars.

  1. Estatísticas dos resultados das análises de laboratório de solo Statistical analysis of a soil testing laboratory results

    Directory of Open Access Journals (Sweden)

    Alberto Cargnelutti Filho

    1996-12-01

    Full Text Available O presente trabalho teve como objetivos estudar as estatísticas dos valores analíticos das análises de solo realizadas no Laboratório de Análise de Solos da Universidade Federal de Santa Maria (LAS/UFSM durante o período de 1988 à 1993 e obter valores como limites críticos que possibilitem um controle de qualidade na emissão dos laudos das análises de solo. Foram utilizadas as 21380 amostras de solos, provenientes de 162 municípios do Rio Grande do Sul. As análises estatísticas/oram realizadas para os 29 municípios que enviaram mais de 150 amostras de solo. Uma evolução distinta dos valores do teor de argila, pH em água, índice SMP, fósforo, potássio, teor de matéria orgânica, alumínio trocável e cálcio + magnésio trocável foi obtida nos diferentes municípios em função do tempo. As estatísticas obtidas, para estas variáveis, podem ser usadas para caracterizar a fertilidade dos solos destes municípios. Os resultados são dispersos e, em geral, não seguem uma distribuição de probabilidades conhecida. Os limites críticos (inferior e superior obtidos podem ser usados como parâmetros para o controle de qualidade nos laudos de análise de solo.The objectives of this research were to study the behaviour and evolution of soil testing results from 1988 to 1993 time period and to define the range for critical values to include in algorithms which may be used for soil testing reports. Results of 21380 soil samples analysis from Federal University of Santa Maria soil testing laboratory were used from 1988 to 1993 time period. The samples carne from 162 counties of Rio Grande do Sul state including 29 counties with more then 150 soil samples. The results for clay content, water pH, SMP Índex, P, K, organic matter and exchangeable Al and Ca+Mg for each county are dispersed and show unknown distribution probabilities. Higher and lower critical values were obteined to analyze quality control for routine soil testing labs.

  2. Analysis on laboratory examination results in old people aged 65 and above in a town of Chongqing%重庆市某乡镇65岁及以上老年人实验室体检结果分析

    Institute of Scientific and Technical Information of China (English)

    潘静

    2014-01-01

    目的:分析重庆市北碚区歇马镇65岁及以上老年人实验室体检结果,了解当地老年人的健康状况。方法对2013年在该院健康体检的1751例65岁及以上老年人的实验室检查结果(包括血、尿常规,肝、肾功能,血脂和血糖)进行分析。结果主要指标异常检出率由高到低依次为三酰甘油、血糖、胆固醇、尿素氮、肌酐、丙氨酸氨基转移酶异常;男性尿素氮、肌酐异常高于女性,女性胆固醇异常高于男性;胆固醇异常65~70岁、>70~80岁年龄组高于80岁以上年龄组,尿素氮、肌酐异常80岁以上年龄组高于65~70岁、>70~80岁年龄组,差异均有统计学意义(P<0.05)。结论通过对老年人定期开展健康体检和健康教育,可以降低慢性病的发病率,提高生活质量。%Objective To analyze the results of laboratory examination in old people aged 65 and above in Xiema town of Beibei district of Chongqing,so as to understand the health state of the local old people. Methods Analysis on laboratory exami-nation results of health check-up was conducted among 1 751 old people aged 65 and above in 2013 in the health center ,includ-ing blood,urine routine,liver and renal function,blood lipid and blood glucose. Results The main indicators of abnormal detec-tion rate from high to low were triglyceride,blood glucose,cholesterol,urea nitrogen,creatinine and alanine transaminase;the ab-normal detection rates of urea nitrogen and creatinine of the male were higher than those of the female ,but the detection rate of abnormal cholesterol of the female was higher than that of the male;the abnormal detection rate of cholesterol in the people with the age of 65-70 and>70-80 years was higher than that of the people with the age over 80 years ,and the abnormal detection rates of urine nitrogen and creatinine of the people with the age over 80 years were higher than those of the people with the age of 65-70 and>70

  3. Parsing abnormal grain growth in specialty aluminas

    Science.gov (United States)

    Lawrence, Abigail Kremer

    multivariate statistical tool called canonical correlation analysis was adopted to seek out relationships between a set of input variables and the abnormal character values. The input variables include the MgO, CaO, Na 2O, and SiO2 contents, the ratio of MgO:(CaO+SiO2), and the annealing time and temperature. The analysis was applied to 33 different samples and showed that the composition ratio and MgO content were the strongest processing variables. These variables are most closely related to the correlation between grain size and aspect ratio, the average magnitude of abnormality, and the variance in grain size. The physical implications of these relationships are explored for a number of samples with different abnormal grain growth behaviors. Several of the samples contained a beta"-alumina phase that is shown to have a dampening effect on abnormal grain growth. TEM investigation provides evidence that there is a grain boundary complexion with a different composition and structure than the second phase. A series of samples are compared after annealing for different times and are shown to have very different behaviors as a result of the second phase competing with complexions for control over the microstructure.

  4. Pregnancy Complications: Umbilical Cord Abnormalities

    Science.gov (United States)

    ... defects. These tests may include a detailed ultrasound, amniocentesis (to check for chromosomal abnormalities) and in some ... the provider may recommend additional tests, such as amniocentesis and a detailed ultrasound, to diagnose or rule ...

  5. Elevated frequency of abnormalities in barn swallows from Chernobyl.

    Science.gov (United States)

    Møller, A P; Mousseau, T A; de Lope, F; Saino, N

    2007-08-22

    Ever since the Chernobyl accident in 1986, that contaminated vast areas in surrounding countries with radiation, abnormalities and birth defects have been reported in human populations. Recently, several studies suggested that the elevated frequency of such abnormalities can be attributed to poverty and stress in affected human populations. Here, we present long-term results for a free-living population of barn swallows, Hirundo rustica, demonstrating the presence of 11 morphological abnormalities in populations around Chernobyl, but much less frequently in an uncontaminated Ukrainian control population and three more distant control populations. The presence of these abnormalities in barn swallows is associated with reduced viability. These findings demonstrate a link between morphological abnormalities and radiation in an animal population that cannot be attributed to poverty and stress. The most parsimonious hypothesis for abnormalities in animal and human populations alike is that the effects are caused by the same underlying cause, viz. radiation derived from the Chernobyl accident.

  6. Abnormal grain growth in undoped strontium and barium titanate

    Energy Technology Data Exchange (ETDEWEB)

    Baeurer, M., E-mail: m.baeurer@ikm.uka.de [Institut fuer Keramik im Maschinenbau, Universitaet Karlsruhe, Karlsruhe (Germany); Shih, S.-J.; Bishop, C. [Department of Materials, University of Oxford, Oxford (United Kingdom); Harmer, M.P. [Center for Advanced Materials and Nanotechnology, Lehigh University, Bethlehem, PA (United States); Cockayne, D. [Department of Materials, University of Oxford, Oxford (United Kingdom); Hoffmann, M.J. [Institut fuer Keramik im Maschinenbau, Universitaet Karlsruhe, Karlsruhe (Germany)

    2010-01-15

    Abnormal grain growth is a commonly observed phenomenon in perovskite materials. In order to study this phenomenon, grain growth experiments were conducted over a temperature range from 1425 to 1600 deg. C for the model system SrTiO{sub 3} to analyse the nucleation of abnormal grains and to identify the growth mechanism involved for normal and abnormal grains. Grain boundaries of normal and abnormal grains were investigated in quenched samples by high-resolution transmission electron microscopy and by energy-dispersive spectroscopy in a scanning transmission electron microscope. No amorphous film was observed at the grain boundaries for either normal or abnormal grains. Non-stoichiometry at the grain boundaries was identified as a possible reason for the differences in growth speed. The results are compared to the nucleation and growth of abnormal grains in BaTiO{sub 3}.

  7. How Does Structured Sparsity Work in Abnormal Event Detection?

    DEFF Research Database (Denmark)

    Ren, Huamin; Pan, Hong; Olsen, Søren Ingvor

    behavior. Otherwise, it should be detected as an abnormal frame. However, it is infeasible to apply structured sparsity algorithms directly in abnormal event detection, which are mainly due to two reasons: 1) abnormal event detection has a highly biased training data - only normal videos are used during...... the training, which is the due to the fact that abnormal videos are limited or even unavailable in advance in most video surveillance applications. As a result, there could be only one label in the training data which hampers supervised learning; 2) Even though there are multiple types of normal behaviors, how...... structure learned from the training data could have a high bias and ruin the precision of abnormal event detection. Therefore, we in the paper propose an algorithm to solve the abnormality detection problem by sparse representation, in which local structured sparsity is preserved in coefficients. To better...

  8. Establishing a cost-per-result of laboratory-based, reflex Cryptococcal antigenaemia screening (CrAg) in HIV+ patients with CD4 counts less than 100 cells/μl using a Lateral Flow Assay (LFA) at a typical busy CD4 laboratory in South Africa.

    Science.gov (United States)

    Cassim, Naseem; Schnippel, Kathryn; Coetzee, Lindi Marie; Glencross, Deborah Kim

    2017-01-01

    Cryptococcal meningitis is a major cause of mortality and morbidity in countries with high HIV prevalence, primarily affecting patients whose CD4 are results are positive. A laboratory-based reflexed CrAg screening approach, using a Lateral Flow Assay (LFA) on remnant EDTA CD4 blood samples, was piloted at three CD4 laboratories. This study aimed to assess the cost-per-result of laboratory-based reflexed CrAg screening at one pilot CD4 referral laboratory. CD4 test volumes from 2014 were extracted to estimate percentage of CD4 result was estimated using a bottom-up method, inclusive of test kits and consumables (reagents), laboratory equipment and technical effort costs. The ZAR/$ exchange of 14.696/$1 was used, where applicable. One-way sensitivity analyses on the cost-per-result were conducted for possible error rates (3%- 8%, reductions or increases in reagent costs as well as test volumes (ranging from -60% to +60%). The pilot CD4 laboratory performed 267000 CD4 tests in 2014; ~ 9.3% (27500) reported CD4result of $4.28 was reported, with reagents contributing $3.11 (72.8%), while technical effort and laboratory equipment overheads contributed $1.17 (27.2%) and $0.03 (result range of $3.84 to $6.03. A cost-per-result of $4.28 was established in a typical CD4 service laboratory to enable local budgetary cost projections and programmatic cost-effectiveness modelling. Varying reagent costs linked to currency exchange and varying test volumes in different levels of service can lead to varying cost-per-test and technical effort to manage workload, with an inverse relationship of higher costs expected at lower volumes of tests.

  9. The CheMin XRD on the Mars Science Laboratory Rover Curiosity: Construction, Operation, and Quantitative Mineralogical Results from the Surface of Mars

    Science.gov (United States)

    Blake, David F.

    2015-01-01

    The Mars Science Laboratory mission was launched from Cape Canaveral, Florida on Nov. 26, 2011 and landed in Gale crater, Mars on Aug. 6, 2012. MSL's mission is to identify and characterize ancient "habitable" environments on Mars. MSL's precision landing system placed the Curiosity rover within 2 km of the center of its 20 X 6 km landing ellipse, next to Gale's central mound, a 5,000 meter high pile of laminated sediment which may contain 1 billion years of Mars history. Curiosity carries with it a full suite of analytical instruments, including the CheMin X-ray diffractometer, the first XRD flown in space. CheMin is essentially a transmission X-ray pinhole camera. A fine-focus Co source and collimator transmits a 50µm beam through a powdered sample held between X-ray transparent plastic windows. The sample holder is shaken by a piezoelectric actuator such that the powder flows like a liquid, each grain passing in random orientation through the beam over time. Forward-diffracted and fluoresced X-ray photons from the sample are detected by an X-ray sensitive Charge Coupled Device (CCD) operated in single photon counting mode. When operated in this way, both the x,y position and the energy of each photon are detected. The resulting energy-selected Co Kalpha Debye-Scherrer pattern is used to determine the identities and amounts of minerals present via Rietveld refinement, and a histogram of all X-ray events constitutes an X-ray fluorescence analysis of the sample.The key role that definitive mineralogy plays in understanding the Martian surface is a consequence of the fact that minerals are thermodynamic phases, having known and specific ranges of temperature, pressure and composition within which they are stable. More than simple compositional analysis, definitive mineralogical analysis can provide information about pressure/temperature conditions of formation, past climate, water activity and the like. Definitive mineralogical analyses are necessary to establish

  10. Reliability of nucleic acid amplification methods for detection of Chlamydia trachomatis in urine: results of the first international collaborative quality control study among 96 laboratories

    NARCIS (Netherlands)

    R.P.A.J. Verkooyen (Roel); G.T. Noordhoek; P.E. Klapper; J. Reid; J. Schirm; G.M. Cleator; M. Ieven; G. Hoddevik

    2003-01-01

    textabstractThe first European Quality Control Concerted Action study was organized to assess the ability of laboratories to detect Chlamydia trachomatis in a panel of urine samples by nucleic acid amplification tests (NATs). The panel consisted of lyophilized urine samples, includ

  11. Occult intraspinal abnormalities and congenital scoliosis

    Directory of Open Access Journals (Sweden)

    Mohammad Ali Erfani

    2007-06-01

    Full Text Available

    BACKGROUND: Congenital scoliosis occurs because of either the failure of formation or the failure of segmentation or both. Evaluation of the incidence and the types of occult intraspinal abnormalities in congenital scoliosis is the subject of this study.

    METHODS: During a period of 29 years, 103 patients with congenital scoliosis were studied. MRI was used in 46 patients, myelography or CT myelography was used in 64 patients and both MRI and myelography or CT myelography were used in 7 patients for intraspinal abnormalities.

    RESULTS: In the MRI group, among the 46 patients, 19 patients (41.3% had intraspinal abnormalities consisting syringomyelia in 9 (19.5% diastematomyelia in 8 (17.4%, tethered cord syndrome in 6 (13%, low conus in 5 (10.8% and diplomyelia in 3 (6.5% of the patients. In the myelography group, among the 64 patients, 17 (26.5% had intraspinal abnormalities and diastematomyelia was the most common one found in 14 (21.8% patients.

    CONCLUSIONS: Intraspinal abnormalities are frequent in congenital scoliosis. Syringomyelia may be associated with congenital scoliosis. In congenital scoliosis, rib fusion may be an indicator of intraspinal abnormalities in MRI. A significant difference between clinical findings and intraspinal anomalies (P<0.05 was noted. Moreover, we believe that total spinal MRI with coronal, sagittal and axial views is a valuable tool in determining the intraspinal abnormalities in congenital scoliosis. This method is highly

  12. Sonographic Gallbladder Abnormality Is Associated with Intravenous Immunoglobulin Resistance in Kawasaki Disease

    Directory of Open Access Journals (Sweden)

    Chih-Jen Chen

    2012-01-01

    Full Text Available Objective. Kawasaki disease (KD is an acute systematic vasculitis in children which causes coronary arterial lesions and hydrops of gallbladder. Our objective is to correlate the clinical significance and influence on disease outcome of patients with gallbladder abnormalities in Kawasaki dissease. Methods. Children who met KD diagnosis criteria and were admitted for IVIG treatment were retrospectively enrolled for analysis. Patients with abdominal sonography were divided into 2 groups based on the absence (Group A, N=61 or presence (Group B, N=16 of gallbladder abnormalities (GBA, defined as hydrops or acalculous cholecystitis. Between the two groups, clinical features, demographic data (including admission days, coronary artery lesions, IVIG resistance, and laboratory data before/after IVIG treatment were collected for analysis. Results. The presence of sonographic gallbladder abnormalities is correlated with higher levels of serum CRP, GPT, and neutrophils. It also points to an increased number of IVIG resistance rates in group B. There was no significant statistical difference among clinical features, age, gender, admission days, or coronary artery lesions between the two groups. Conclusion. Sonographic gallbladder abnormalities are associated with higher CRP, GPT, neutrophil and IVIG resistance in KD. It can be used as a predictor of IVIG resistance in patients with KD.

  13. Positive Reinforcement Training Moderates Only High Levels of Abnormal Behavior in Singly Housed Rhesus Macaques

    Science.gov (United States)

    Baker, Kate C.; Bloomsmith, Mollie; Neu, Kimberly; Griffis, Caroline; Maloney, Margaret; Oettinger, Brooke; Schoof, Valérie A. M.; Martinez, Marni

    2010-01-01

    This study evaluated the application of positive reinforcement training (PRT) as an intervention for abnormal behaviors in singly housed laboratory rhesus macaques at 2 large primate facilities. Training involved basic control behaviors and body-part presentation. The study compared baseline behavioral data on 30 adult males and 33 adult females compared with 3 treatment phases presented in counterbalanced order: 6 min per week of PRT, 20 or 40 min per week of PRT, and 6 min per week of unstructured human interaction (HI). Within-subject parametric tests detected no main or interaction effects involving experimental phase. However, among a subset of subjects with levels of abnormal in the top quartile of the range (n = 15), abnormal behavior was reduced from 35% to 25% of samples with PRT but not with HI. These results suggest that short durations of PRT applied as enrichment for this species and in this context may not in itself be sufficient intervention for abnormal behavior because levels remained high. However, it may be appropriate as an adjunct to other interventions and may be best targeted to the most severely affected individuals. PMID:20183477

  14. Prevalence of pathogens of abnormal vaginal discharges in a Nigerian tertiary hospital

    Institute of Scientific and Technical Information of China (English)

    Samue Nwadioha; Julie O Egesie; Henry Emejuo; Elizabeth Iheanacho

    2010-01-01

    Objective:To investigate the prevalence of pathogens of abnormal vaginal discharges and to improve the current syndromic management of abnormal vaginal discharge. Methods:A prospective study of pathogens of abnormal vaginal discharge was carried out from December 2007 to December 2008. Samples of female genital swabs were collected from Obstetrics and Gynecology Units of Aminu Kano Teaching Hospital, Kano, Nigeria, and analyzed by microscopy, culture and sensitivity test in Medical Microbiology Laboratory of Aminu Kano Teaching Hospital. Results:Microorganisms were detected in 70%(1 400) of a total 2 000 female genital swabs studied. Candida species peaked with 42.0% (840), followed by Gardnerella vaginalis, a pathogen of bacteria vaginosis with 26.0%. The distribution of abnormal vaginal discharge was highest in young adults aged from 21 to 30 years. Conclusions:The commonest Microorganisms of infective vaginal discharge were Candida species, followed by Gardnerella vaginalis, a pathogen of bacterial vaginosis. Vaginal discharge was prevalent among young adults. We recommend prevention, early diagnosis and prompt treatment of infected female, especially among the young, sexually active group in order to reduce the menace of HIV transmission.

  15. Positive reinforcement training moderates only high levels of abnormal behavior in singly housed rhesus macaques.

    Science.gov (United States)

    Baker, Kate C; Bloomsmith, Mollie; Neu, Kimberly; Griffis, Caroline; Maloney, Margaret; Oettinger, Brooke; Schoof, Valerie A M; Martinez, Marni

    2009-01-01

    This study evaluated the application of positive reinforcement training (PRT) as an intervention for abnormal behaviors in singly housed laboratory rhesus macaques at 2 large primate facilities. Training involved basic control behaviors and body-part presentation. The study compared baseline behavioral data on 30 adult males and 33 adult females compared with 3 treatment phases presented in counterbalanced order: 6 min per week of PRT, 20 or 40 min per week of PRT, and 6 min per week of unstructured human interaction (HI). Within-subject parametric tests detected no main or interaction effects involving experimental phase. However, among a subset of subjects with levels of abnormal in the top quartile of the range (n = 15), abnormal behavior was reduced from 35% to 25% of samples with PRT but not with HI. These results suggest that short durations of PRT applied as enrichment for this species and in this context may not in itself be sufficient intervention for abnormal behavior because levels remained high. However, it may be appropriate as an adjunct to other interventions and may be best targeted to the most severely affected individuals.

  16. Hemostatic abnormalities in liver cirrhosis

    Directory of Open Access Journals (Sweden)

    Kendal YALÇIN

    2009-06-01

    Full Text Available In this study, 44 patients with liver cirrhosis were investigated for hemostatic parameters. Patients with spontaneous bacterial peritonitis, hepatocellular carcinoma, hepatorenal syndrome and cholestatic liver diseases were excluded. Patients were classified by Child-Pugh criterion and according to this 4 patients were in Class A, 20 in Class B and 20 in C. Regarding to these results, it was aimed to investigate the haematological disturbances in liver cirrhotic patients.In the result there was a correlation between activated partial thromboplastin time, serum iron, ferritin, transferrin, haptoglobin and Child-Pugh classification. Besides there was no correlation between prothrombin time, factor 8 and 9, protein C and S, anti-thrombin 3, fibrinogen, fibrin degradation products, serum iron binding capacity, hemoglobin, leukocyte, mean corpuscular volume and Child-Pugh classification.There were significant difference, in terms of AST, ferritin, haptoglobulin, sex and presence of ascites between groups (p0.05. In the summary, we have found correlation between hemostatic abnormalities and disease activity and clinical prognosis in patients with liver cirrhosis which is important in the management of these patients. This is also important for identification of liver transplant candidiates earlier.

  17. Computational Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — This laboratory contains a number of commercial off-the-shelf and in-house software packages allowing for both statistical analysis as well as mathematical modeling...

  18. Analytical Laboratories

    Data.gov (United States)

    Federal Laboratory Consortium — NETL’s analytical laboratories in Pittsburgh, PA, and Albany, OR, give researchers access to the equipment they need to thoroughly study the properties of materials...

  19. Geomechanics Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Geomechanics Laboratory allows its users to measure rock properties under a wide range of simulated service conditions up to very high pressures and complex load...

  20. Real-time Multiple Abnormality Detection in Video Data

    DEFF Research Database (Denmark)

    Have, Simon Hartmann; Ren, Huamin; Moeslund, Thomas B.

    2013-01-01

    Automatic abnormality detection in video sequences has recently gained an increasing attention within the research community. Although progress has been seen, there are still some limitations in current research. While most systems are designed at detecting specific abnormality, others which...... are capable of detecting more than two types of abnormalities rely on heavy computation. Therefore, we provide a framework for detecting abnormalities in video surveillance by using multiple features and cascade classifiers, yet achieve above real-time processing speed. Experimental results on two datasets...

  1. Craniofacial abnormalities among patients with Edwards Syndrome

    Directory of Open Access Journals (Sweden)

    Rafael Fabiano M. Rosa

    2013-09-01

    Full Text Available OBJECTIVE To determine the frequency and types of craniofacial abnormalities observed in patients with trisomy 18 or Edwards syndrome (ES. METHODS This descriptive and retrospective study of a case series included all patients diagnosed with ES in a Clinical Genetics Service of a reference hospital in Southern Brazil from 1975 to 2008. The results of the karyotypic analysis, along with clinical data, were collected from medical records. RESULTS: The sample consisted of 50 patients, of which 66% were female. The median age at first evaluation was 14 days. Regarding the karyotypes, full trisomy of chromosome 18 was the main alteration (90%. Mosaicism was observed in 10%. The main craniofacial abnormalities were: microretrognathia (76%, abnormalities of the ear helix/dysplastic ears (70%, prominent occiput (52%, posteriorly rotated (46% and low set ears (44%, and short palpebral fissures/blepharophimosis (46%. Other uncommon - but relevant - abnormalities included: microtia (18%, orofacial clefts (12%, preauricular tags (10%, facial palsy (4%, encephalocele (4%, absence of external auditory canal (2% and asymmetric face (2%. One patient had an initial suspicion of oculo-auriculo-vertebral spectrum (OAVS or Goldenhar syndrome. CONCLUSIONS: Despite the literature description of a characteristic clinical presentation for ES, craniofacial alterations may be variable among these patients. The OAVS findings in this sample are noteworthy. The association of ES with OAVS has been reported once in the literature.

  2. Eclampsia and abnormal QTc

    African Journals Online (AJOL)

    rected for heart rate (QTc), serum calcium, magnesium and potassium ... women with uncomplicated pregnancy. Results: .... supplementation has been found useful in the prevention ... some of the causes of high mortality in eclampsia. We.

  3. Cytogenetic Analysis for Suspected Chromosomal Abnormalities; A Five Years Experience

    Science.gov (United States)

    Karra, Vijay Kumar; Jindal, Ankur; Puppala, Madhavi; Singh, Pratiksha; Rawat, Kanchan; Kapoor, Seema

    2016-01-01

    Introduction Chromosomal abnormalities are the results of alterations in the number or structure of chromosomes causing significant human morbidity and mortality. They are responsible for a large proportion of miscarriages, developmental delay, disorders of sexual development, congenital malformations and mental retardation. Aim The aim of this study was to describe the prevalence of different chromosomal abnormalities in North Indian patients referred for cytogenetic analysis. Materials and Methods Total of 859 patients ranging from newborn to 37 years of age were referred to the division of genetics, Department of Paediatrics between 2010 and 2015, with a variety of clinical disorders; Down syndrome (DS), Turner’s syndrome (TS) and Klinefelter syndrome; amenorrhea; ambiguous sex and multiple congenital malformations. Chromosomal analysis was performed on lymphocyte culture according to standard methods. Results Of the 859 cases studied, 371 (43.1%) had chromosomal abnormalities. The most common autosomal abnormalities were DS 302 (81.4%) and sex chromosomal abnormalities were TS 51 (13.7%). Numerical abnormalities were accounted for 353 (41.0%) and structural abnormalities 18 (2.0%), respectively. Various other chromosomal anomalies were also reported. Conclusion We have reviewed the incidence and distribution of chromosomal abnormalities and found higher rate of chromosomal abnormalities 43.1% in the referred cases. Our data suggest that chromosomal analysis is important tool in the evaluation of genetic disorders and helps clinicians to provide accurate diagnosis and proper genetic counselling. PMID:27790464

  4. Abnormal cervical cytology and health care use

    DEFF Research Database (Denmark)

    Frederiksen, Maria Eiholm; Baillet, Miguel Vázquez-Prada; Dugué, Pierre-Antoine

    2015-01-01

    OBJECTIVE: This study aimed to assess the long-term use of health care services in women with abnormal cytology results compared to women with normal cytology results. METHODS: We did a nationwide population-based study, using women aged 23 to 59years participating in the national organized...... cervical cancer screening program. We included a study population of 40,153 women with abnormal cytology (exposed) and 752,627 women with normal cytology (non-exposed). We retrieved data from the Danish Civil Registration System, the Danish Pathology Data Bank, the National Health Service, the National......" the cytology result and for the 5-year period "after" the result. RESULTS: During the "before" period exposed women had more contacts to GPs, more contacts to psychologists/psychiatrist, and more hospital admissions than non-exposed women. In both exposed and non-exposed women, health care use increased from...

  5. Establishing and validating luminiscence dating procedures for archaoelogical remains in the geochronology laboratory of the University of A Coruña: first results

    Directory of Open Access Journals (Sweden)

    Sanjurjo Sánchez, Jorge

    2008-12-01

    Full Text Available Thermoluminescence dating (TL of archaeological material is a technique about 50 years old, but it is not much used in Spain. Recent methodological advances have improved the accuracy and precision of the method. The unit of Geochronology of the University Geological Institute “Isidro Parga Pondal” University of A Coruña, has set up recently a Luminescence laboratory. In order to test analytical dating procedures, medieval tiles from an archaeological site next to the Hercules Tower (A Coruña have been dated. The stratigraphical column was previously dated by 14C, so a good chronological control is available. Samples were analysed using two different analytical procedures: a classical one using a multialiquot approach (AD-TL and a recent one using a single aliquot procedure (SAR-TL. Our results show that both methods yield comparable paleodoses, being the SAR-TL the one with smaller error.

    La datación mediante termoluminiscencia (TL de material arqueológico es una técnica con unos 50 años de antigüedad, aunque no muy empleada en España. Avances metodológicos recientes han permitido aumentar la exactitud y precisión del método. La Unidad de Geocronología del Instituto Universitario de Xeoloxía “Isidro Parga Pondal” de la Universidad de A Coruña cuenta en la actualidad con un Laboratorio de Luminiscencia. Para poner a punto los procedimientos analíticos de datación, se han datado tejas medievales de una excavación cercana a la Torre de Hércules (A Coruña en la que la columna estratigráfica fue datada por medio de 14C. Las muestras fueron sometidas a dos procedimientos existentes en la literatura: uno clásico (AD-TL y otro de reciente desarrollo (SAR-TL. Los resultados muestran concordancia entre ambos métodos e incluso mejoras considerables obtenidas con el SAR-TL.

  6. 19. The HUman Micro Nucleus project. International Date Base Comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes. Ⅰ. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The first results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus assay in human lymphocytes and participating in the HUMN (HUman MicroNucleus project) international collaborative study are presented. The effects of laboratory protocol, scoring criteria, and host factors on baseline micronucleus(MN) frequency are evaluated, and a reference range of “normal” values against which future studies may be compared is provided. Primary data from historical records were submitted by 25 laboratories distributed in 16 countries. This resulted in a database of nearly 7000 subjects. Potentially significant differences were present in the methods used by participating laboratories, such as in the type of culture medium, the concentration of Cytochalasin-B, the percentage of fetal calf serum, and in the culture method. Differences in criteria for scoring MN were also evident. The overall median MN frequency in non-exposed(i.e., normal) subjects was 6.5‰ and the interquartile range was between 3‰ and 12‰. An increase in MN frequency with age was evident in all but two laboratories. The effect of gender, although not so evident in all databases, was also present, with females having a 19% higher level of MN (95% C.I.:14-24%). Statistical analyses were performed using random-effects models for correlated data. Our best model, which included exposure to genotoxic factors, host factors, methods, and scoring criteria, explained 75% of the total variance, with the largest contribution attributable to laboratory methods.

  7. Cytogenetic abnormalities and fragile-x syndrome in Autism Spectrum Disorder

    Directory of Open Access Journals (Sweden)

    Reddy Kavita S

    2005-01-01

    Full Text Available Abstract Background Autism is a behavioral disorder with impaired social interaction, communication, and repetitive and stereotypic behaviors. About 5–10 % of individuals with autism have 'secondary' autism in which an environmental agent, chromosome abnormality, or single gene disorder can be identified. Ninety percent have idiopathic autism and a major gene has not yet been identified. We have assessed the incidence of chromosome abnormalities and Fragile X syndrome in a population of autistic patients referred to our laboratory. Methods Data was analyzed from 433 patients with autistic traits tested using chromosome analysis and/or fluorescence in situ hybridization (FISH and/or molecular testing for fragile X syndrome by Southern and PCR methods. Results The median age was 4 years. Sex ratio was 4.5 males to 1 female [354:79]. A chromosome (cs abnormality was found in 14/421 [3.33 %] cases. The aberrations were: 4/14 [28%] supernumerary markers; 4/14 [28%] deletions; 1/14 [7%] duplication; 3/14 [21%] inversions; 2/14 [14%] translocations. FISH was performed on 23 cases for reasons other than to characterize a previously identified cytogenetic abnormality. All 23 cases were negative. Fragile-X testing by Southern blots and PCR analysis found 7/316 [2.2 %] with an abnormal result. The mutations detected were: a full mutation (fM and abnormal methylation in 3 [43 %], mosaic mutations with partial methylation of variable clinical significance in 3 [43%] and a permutation carrier [14%]. The frequency of chromosome and fragile-X abnormalities appears to be within the range in reported surveys (cs 4.8-1.7%, FRAX 2–4%. Limitations of our retrospective study include paucity of behavioral diagnostic information, and a specific clinical criterion for testing. Conclusions Twenty-eight percent of chromosome abnormalities detected in our study were subtle; therefore a high resolution cytogenetic study with a scrutiny of 15q11.2q13, 2q37 and Xp23

  8. Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization

    DEFF Research Database (Denmark)

    Kwasny, Dorota; Vedarethinam, Indumathi; Shah, Pranjul

    2012-01-01

    Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cy...

  9. Laboratory Activities

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Christopher F.; Serne, R. Jeffrey

    2008-01-17

    This chapter summarizes the laboratory activities performed by PNNL’s Vadose Zone Characterization Project in support of the Tank Farm Vadose Zone Program, led by CH2M HILL Hanford Group, Inc. The results of these studies are contained in numerous reports (Lindenmeier et al. 2002; Serne et al. 2002a, 2002b, 2002c, 2002d, 2002e; Lindenmeier et al. 2003; Serne et al. 2004a, 2004b; Brown et al. 2005, 2006a, 2007; Serne et al. 2007) and have generated much of the data reported in Chapter 22 (Geochemistry-Contaminant Movement), Appendix G (Geochemistry-Contaminant Movement), and Cantrell et al. (2007, SST WMA Geochemistry Data Package – in preparation). Sediment samples and characterization results from PNNL’s Vadose Zone Characterization Project are also shared with other science and technology (S&T) research projects, such as those summarized in Chapter 12 (Associated Science Activities).

  10. [Diagnosticum of abnormalities of plant meiotic division].

    Science.gov (United States)

    Shamina, N V

    2006-01-01

    Abnormalities of plant meiotic division leading to abnormal meiotic products are summarized schematically in the paper. Causes of formation of monads, abnormal diads, triads, pentads, polyads, etc. have been observed in meiosis with both successive and simultaneous cytokinesis.

  11. Analysis of the results obtained for proficiency testing schemes in laboratories for food control in Institute of Public Health, Skopje, FYROM

    OpenAIRE

    Kostik, Vesna; Memeti, Shaban; Mickov, Toni; Kiroska-Petreska, Evgenija

    2011-01-01

    Within the period of 9 years (2002 – 2010) in the laboratories for food testing in Institute of Public Health, Skopje, FYROM, 32 different proficiency tests were performed in 23 different matrixes as: coffee cream, cream, powdered milk, yoghurt butter; oily dressing, cola drink, cabbage puree , fresh cheese , orange juice, potable water, water real sample, animal feed cereal based, fish oil, maize flour, corn flakes, minced canned fish, graham flour, synthetic food of vegetable and animal ori...

  12. NOx abatement by fuel-lean reburning: Laboratory combustor and pilot-scale package boiler results. Report for June 1995--December 1997

    Energy Technology Data Exchange (ETDEWEB)

    Miller, C.A.; Touati, A.D.; Becker, J.; Wendt, J.O.L.

    1997-12-31

    The paper discusses two experimental studies related to the abatement of nitrogen oxides (NOx) by fuel-lean reburning. First, systematic tests in a 17-kW down-flow laboratory combustor, in which nitric oxide (NO) in the oxidant was destroyed in long, axial, methane/air diffusion flames, showed that substantial reduction of NO was possible under overall fuel-lean conditions.

  13. The Pea Seedling as a Model of Normal and Abnormal Morphogenesis

    Science.gov (United States)

    Kurkdjian, Armen; And Others

    1974-01-01

    Describes several simple and inexpensive experiments designed to facilitate the study of normal and abnormal morphogenesis in the biology laboratory. Seedlings of the common garden pea are used in the experiments, and abnormal morphogenesis (tumors) are induced by a virulent strain of the crown-gall organism, Agrobacterium tumefaciens. (JR)

  14. Renal abnormalities in patients with Kallmann syndrome.

    Science.gov (United States)

    Zenteno, J C; Méndez, J P; Maya-Núñez, G; Ulloa-Aguirre, A; Kofman-Alfaro, S

    1999-03-01

    To report experience in patients with Kallmann syndrome (KS) in whom urography was used to establish the type and frequency of renal anomalies associated with the disorder. Of 19 patients with KS, 15 had the X-linked recessive form of the disease, whereas the remaining four were sporadic. Each patient underwent intravenous pyelography (IVP) using a non-ionic, low osmolarity contrast medium. Of the 19 patients with KS, 10 had kidney abnormalities; four presented with unilateral renal agenesis and six had less severe forms of renal abnormality (renal malrotation in four and bilateral dilatation of the calyces and pelves in two). One of the patients with unilateral renal agenesis carried a deletion in KAL, the gene responsible for the X-linked type of KS. Three of the four patients with renal malrotation had a confirmed X-linked recessive form and one carried a point mutation in KAL. These results suggest that kidney abnormalities are more frequent and diverse in patients with KS than previously reported. They also indicate that defects in the KAL gene may contribute to abnormal renal development. However, a review of the literature revealed no close correlation between KAL mutations and kidney anomalies in the X-linked type of disease. Taken together, these data suggest that KAL mutations are not invariably associated with failure of renal development and that additional factors (epigenetic or local) may compensate for defects in the KAL protein.

  15. Virtual Laboratories

    Science.gov (United States)

    Hut, P.

    At the frontier of most areas in science, computer simulations playa central role. The traditional division of natural science into experimental and theoretical investigations is now completely outdated. Instead, theory, simulation, and experimentation form three equally essential aspects, each with its own unique flavor and challenges. Yet, education in computational science is still lagging far behind, and the number of text books in this area is minuscule compared to the many text books on theoretical and experimental science. As a result, many researchers still carry out simulations in a haphazard way, without properly setting up the computational equivalent of a well equipped laboratory. The art of creating such a virtual laboratory, while providing proper extensibility and documentation, is still in its infancy. A new approach is described here, Open Knowledge, as an extension of the notion of Open Source software. Besides open source code, manuals, and primers, an open knowledge project provides simulated dialogues between code developers, thus sharing not only the code, but also the motivations behind the code.

  16. Abnormal behavior in caged birds kept as pets.

    Science.gov (United States)

    van Hoek, C S; ten Cate, C

    1998-01-01

    There are a limited number of studies dealing with abnormal behavior in caged birds kept as pets. However, these studies demonstrate the presence of abnormal behavior in both songbirds and parrots. Ethological studies on these birds, as well as studies on domestic and zoo birds, indicate that inappropriate rearing and housing conditions may lead to behavioral abnormalities. Together these data indicate that behavioral abnormalities occur among both wild-caught and domesticated pet birds. The severity and magnitude of these abnormalities is probably underestimated, and there is a need for systematic studies on the nature, origin, variability, species-specificity, and reversibility of behavioral problems in pet birds. Abnormal behavior in caged birds may to some extent be prevented and reduced by environmental enrichment. However, most enrichment studies are anecdotal and not based on a thorough analysis of the behavioral abnormalities, which may lead to measures resulting in a reduction of symptoms rather than the underlying causes. Although it is likely that several of these problems could be reduced by modifying rearing and housing conditions, the current insights into the causal mechanisms underlying abnormal behavior of domesticated and wild-caught pet birds are limited, as are the insights into the possibilities of preventing or curing abnormal behavior.

  17. Abnormal Retained Earnings Around The World

    OpenAIRE

    Alves, Paulo; Silva,Paulo

    2017-01-01

    Using a firm-level survey database covering 50 countries we evaluate firms´ abnormal retained earnings. The results of our work indicate that firms located in emerging markets retain more earnings than firms from developed countries. On the other hand, firms located on common law based countries retain earnings above the expected and higher than firms placed on civil law based countries. A possible explanation, according to our results, can be seen in the economic growth that these countries ...

  18. Analysis on laboratory results characteristics of dengue fever%登革热实验室检测的结果分析

    Institute of Scientific and Technical Information of China (English)

    肖倩; 邱峰; 曾建明; 石文; 李有强; 张轩; 陈茶

    2016-01-01

    目的:分析登革热实验室检测病毒核酸、血清丙二醛(MDA)、血常规的特点。方法统计2014年9-10月广东省中医院收治的60例疑似登革热患者的临床及实验室检查结果,采用PCR荧光探针法检测患者的登革病毒核酸, ELISA法进行MDA检测,联合血常规检测进行结果分析。结果本次登革热流行临床表现典型,以高热、疲乏、骨骼肌肉酸痛、皮疹为多见。实验室检测结果为登革热核酸阳性数40例,登革热核酸阴性20例。血清MDA含量在登革热核酸阳性组高于阴性对照组[(2.13±5.41) mmol/L vs (0.99±0.70)mmol/L],男性阳性组高于男性对照组[(3.04±7.22)mmol/L vs (0.97±0.77)mmol/L],女性阳性组高于女性对照组[(1.02±0.68)mmol/L vs (1.01±0.64)mmol/L],但差异均无统计学意义(P>0.05)。白细胞减少21例,血小板减少20例。结论临床特征明显,白细胞、血小板减少为典型表现,开展MDA检测具有良好的应用前景。%Objective To study the feature of viral nucleic acid, serum malonaldehyde(MDA) and blood on dengue patients. Methods The clinical and laboratory results of 60 cases of suspected dengue patients were statistically analyzed. The dengue viral nucleic acid was detected by PCR; MDA was detected by ELISA and combined blood test was performed. Results The typical clinical manifestations of dengue epidemic were with high fever, fatigue, musculoskeletal pain and skin rash. 40 cases were positive for dengue nucleic detection and 20 cases were negative. The serum MDA content of the dengue fever group was higher than that of control group [(2.13±5.41)mmol/L vs (0.99±0.70)mmol/L], the serum MDA content of the male of the positive group was higher than that of control group [(3.04±7.22)mmol/L vs (0.97±0.77)mmol/L]; the serum MDA content of the female-positive group was higher than that of control group [(1.02

  19. Gait Analysis Laboratory

    Science.gov (United States)

    1976-01-01

    Complete motion analysis laboratory has evolved out of analyzing walking patterns of crippled children at Stanford Children's Hospital. Data is collected by placing tiny electrical sensors over muscle groups of child's legs and inserting step-sensing switches in soles of shoes. Miniature radio transmitters send signals to receiver for continuous recording of abnormal walking pattern. Engineers are working to apply space electronics miniaturization techniques to reduce size and weight of telemetry system further as well as striving to increase signal bandwidth so analysis can be performed faster and more accurately using a mini-computer.

  20. Head Impact Laboratory (HIL)

    Data.gov (United States)

    Federal Laboratory Consortium — The HIL uses testing devices to evaluate vehicle interior energy attenuating (EA) technologies for mitigating head injuries resulting from head impacts during mine/...

  1. 2010年饮用水中硫酸盐和铜质控结果分析%Analysis of inner - laboratory comparison results of sulfate and copper in drinking water in 2010

    Institute of Scientific and Technical Information of China (English)

    卢素格; 夏芳; 张榕杰

    2011-01-01

    目的:为了更好的提高全省疾控中心实验室在饮用水方面的检测水平.方法:依照《生活饮用水标准检验方法》(GB/T5750.5/6 - 2006)对应方法对合成水样中硫酸盐和铜进行质控考核.用Z比分数统计评价检测结果,分为满意、可疑和不满意3种.结果:此次对全省18个地市和55个县市疾控中心共73个实验室进行水质检验质量控制考核,硫酸盐测定结果满意的57家,占78.1%;结果可疑的8家,占11.0%;结果不满意的8家,占11.0%.铜测定结果满意的64家,占87.7%;结果可疑的8家,占11.0%.结果不满意的1家,占1.3%.结论:全省各级疾病预防控制中心硫酸盐、铜总体检测能力较高,少数实验室检测能力有待提高.%Objective: In order to well enhance the accuracy of laboratory detection result in drinking water. Methods: The sample was detected according to " Standard examination methods for drinking water" ( GB/T5750.5/6 - 2006) and the detection results were analyzed by "Z - score". Results: Detection results were provided by 73 laboratories of city and county centers for disease control and prevention. The detection results of sulfate were satisfactory in 57 laboratories (78. 1% ) , suspicious in 8 laboratories (11. 0% ) and dissatisfactory in 8 laboratories (11. 0% ) ; while the detection results of copper were satisfactory in 64 laboratories (87.1% ), suspicious in 8 laboratories (11.0%) and dissatisfactory inl laboratory (1. 3% ). Conclusion: The general testing capability on sulfate and copper is relatively high. However, there is still much room for improvement on testing quality level in several laboratories.

  2. Menstrual abnormalities in the adolescent abuse of the birth control pill.

    Science.gov (United States)

    Spence, J E

    1983-01-01

    This paper reviews the physiology of menstrual abnormalities in the adolescent and suggests a management protocol based on this pathophysiological framework. Initial discussion covers the physiology of puberty and menstruation. Menstrual abnormalities occur often in the adolescent. Excessive bleeding is the most dramatic symptom, but the most common complaints concern the frequency, duration, and variability of menstrual flow. Often the oral contraceptive (OC) pill is the panacea for the patient, her family, and the physician. This solution has several advantages. It is attractive, inexpensive, easy to use, and regulates the menstrual cycle. Although menstrual abnormalities are seemingly corrected, the treatment is not physiological. This use of the OC pill may mask the underlying problem and delay the diagnosis of a medical disorder and will interfere with the normal maturation of the hypothalamic-pituitary-ovarian axis, which is necessary for regular menses to occur. The diagnosis of anovulatory bleeding is one of exclusion. Both local and systemic problems can mimic dysfunctional bleeding. Pregnancy must always be considered but is often overlooked in the young teenager. A satisfactory history and physical examination, including a vaginal and/or rectal examination, is paramount to rule out other causes. Coagulation defects may play a significant role in adolescent bleeding abnormalities. Endocrine disorders, including thyroid disease, Cushing's syndrome, hypoprolactinemia, and acromegaly, can result in menstrual abnormalities. After a meticulous history and physical examination, any teenager presenting with abnormal vaginal bleeding should not exhibit any physical abnormality other than the signs and symptoms of blood loss. The initial laboratory tests need not be extensive but must include a hemoglobin estimation, blood smear, platelet count, bleeding time, prothrombin time, partial thromboplastin time, and thyroid function tests. Only the simplest

  3. Monitoring and modelling of thermo-hydro-mechanical processes - main results of a heater experiment at the Mont Terri underground rock laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Ingeborg, G.; Alheid, H.J. [BGR - Federal Institute for Geosciences and Natural Resources, Hannover (Germany); Jockwerz, N. [Gesellschaft fur Anlagen- und Reaktorsicherheit (GRS) - Final Repository Research Division, Braunschweig (Germany); Mayor, J.C. [ENRESA - Empresa Nacional des Residuos Radioactivos, Madrid (Spain); Garcia-Siner, J.L. [AITEMIN -Asociacion para la Investigacion y Desarrollo Industrial de los Recursos Naturales, Madrid, (Spain); Alonso, E. [CIMNE - Centre Internacional de Metodos Numerics en Ingenyeria, UPC, Barcelona (Spain); Weber, H.P. [NAGRA - National Cooperative for the Disposal of Radioactive Waste, Wettingen (Switzerland); Plotze, M. [ETHZ - Swiss Federal Institute of Technology Zurich, IGT, Zurich, (Switzerland); Klubertanz, G. [COLENCO Power Engineering Ltd., Baden (Switzerland)

    2005-07-01

    The long-term safety of permanent underground repositories relies on a combination of engineered and geological barriers, so that the interactions between the barriers in response to conditions expected in a high-level waste repository need to be identified and fully understood. Co-financed by the European Community, a heater experiment was realized on a pilot plant scale at the underground laboratory in Mont Terri, Switzerland. The experiment was accompanied by an extensive programme of continuous monitoring, experimental investigations on-site as well as in laboratories, and numerical modelling of the coupled thermo-hydro-mechanical processes. Heat-producing waste was simulated by a heater element of 10 cm diameter, held at a constant surface temperature of 100 C. The heater element (length 2 m) operated in a vertical borehole of 7 m depth at 4 to 6 m depth. It was embedded in a geotechnical barrier of pre-compacted bentonite blocks (outer diameter 30 cm) that were irrigated for 35 months before the heating phase (duration 18 months) began. The host rock is a highly consolidated stiff Jurassic clay stone (Opalinus Clay). After the heating phase, the vicinity of the heater element was explored by seismic, hydraulic, and geotechnical tests to investigate if the heating had induced changes in the Opalinus Clay. Additionally, rock mechanic specimens were tested in the laboratory. Finally, the experiment was dismantled to provide laboratory specimens of post - heating buffer and host rock material. The bentonite blocks were thoroughly wetted at the time of the dismantling. The volume increase amounted to 5 to 9% and was thus below the bentonite potential. Geo-electrical measurements showed no decrease of the water content in the vicinity of the heater during the heating phase. Decreasing energy input to the heater element over time suggests hence, that the bentonite dried leading to a decrease of its thermal conductivity. Gas release during the heating period occurred

  4. Analysis on abnormal results of personal dose monitoring among radiation workers in Nanjing military area command from 2010-2011%2010-2011年南京军区放射工作人员个人剂量监测异常结果分析

    Institute of Scientific and Technical Information of China (English)

    荣曙; 杨龙; 陈乐如; 毛应华; 金慧英; 赵国良; 朱乐明

    2012-01-01

    目的 对2010-2011年南京军区放射工作人员个人剂量监测中数据异常的原因进行总结分析.方法 采用个人剂量计,对个人剂量监测结果进行统计分析.结果 2010-2011年期间,全区共计125人次个人剂量异常,其中从事介入治疗工作的占57.6%,从事放射诊断工作的仅占2.4%.大部分异常剂量在5mSv以下,且56.8%的剂量异常并非实际受照.导致剂量异常的原因主要有剂量计佩戴不规范(50.4%)、工作量大、接触射线时间长(37.6%),以及操作不规范(4.8%)等.结论 加强防护知识培训,加大监督执法力度,提高个人防护意识,保证数据真实可靠,是下一步工作的重点.%[Objective]To summarize and analyze the abnormal reasons of personal dose monitoring data among radiation workers in Nanjing military area command from 2010-2011. [Methods]By using personal dosimeter, the results of personal dose monitoring were analyzed statistically. [Results]During 2010-2011, a total of 12S person/times were detected for abnormal personal dose in Nanjing military area command. Among them, 57.6% were people worked in department of interventional therapy, and only 2.4% worked in department of radiological diagnosis. Most of abnormal doses were less than SmSv, and 56.8% of the abnormal doses were not caused by the actual exposure. The main reasons for abnormal doses included; the application of personal dosimeters was not standardized (SO. 4%), the workload was heavy and the exposure time was too long (37.6% ), as well as the operation was not standard (4.8%). [Conclusion]It is important to strengthen training of knowledge about radiation protection, enhance the intensity of law enforcement, improve the consciousness about personal radiation protection, and ensure that the data is reliable.

  5. Age-related changes in laboratory values used in the diagnosis of anemia and iron deficiency.

    Science.gov (United States)

    Yip, R; Johnson, C; Dallman, P R

    1984-03-01

    Laboratory results from the Second National Health and Nutrition Examination Survey (NHANES II) were used to define age-related changes in laboratory values that are related to the diagnosis of anemia and iron deficiency. Analyses included Hb, hematocrit, red blood cell count, red cell indices, iron, total iron-binding capacity, transferrin saturation, and erythrocyte protoporphyrin. Computation of median values and 95% ranges by age and sex for each laboratory test were performed on 15,093 subjects between 1 and 74 yr of age who had complete laboratory data on venous blood, after excluding those subjects with an abnormality in one or more of three other laboratory tests. Age-related changes in laboratory measurements, such as those described herein, must be taken into consideration in order to optimize the identification of individuals with anemia and iron deficiency.

  6. Results Assessment of Intercomparison Exercise CSN/CIEMAT-2010 among Spanish National Laboratories of Environmental Radioactivity (Diet Ashes); Evaluacion de la Intercomparacion CSN/CIEMAT-2010 entre los Laboratorios Nacionales de Radiactividad Ambiental (Ceniza de Dieta)

    Energy Technology Data Exchange (ETDEWEB)

    Gasco, C.; Trinidad, J. A.; Llaurado, M.; Suarez, J. A.

    2012-06-08

    This report describes the results assessment of the intercomparison exercise among environmental radioactivity laboratories, organised by Spanish Regulatory Institution (CSN) and prepared and evaluated by UAB and CIEMAT respectively. The exercise has been carried out following the international standards ISO-43 and ISO/IUPAC that provide a useful guide to perform proficiency tests and inter-laboratories comparisons. The selected matrix for this year (2010) was a diet ash obtained from the ashing of a whole fresh diet (breakfast, lunch and dinner), that was enriched with artificial radionuclides (Cs-137, Co-60,Fe-55,Ni-63,Sr-90,Am-241,Pu-238,Pu-239,240 y C-14) and contained natural radionuclides (U-234, U-238, U-natural Th-230, Th-234, Ra-226, Ra-228, Pb-210, Pb-212, Pb-214, Bi-214, Ac-228, Tl-208, K-40) at environmental level of activity concentration. The z-score test was applied to determine how much the laboratories differ from the reference value. The reference value for this exercise was the median of the results from the different laboratories and their standard deviations to achieve a more complete and objective study of the laboratories performance. The participant laboratories have demonstrated a satisfactory quality level for measuring the natural and artificial radionuclides content in this matrix. The reference values obtained through the medians show a negative bias for Pb-210 and Th-234 when comparing to the given values of external qualified laboratories from ENEA and IRSN and positive one for K-40. (Author)

  7. Laboratory Building

    Energy Technology Data Exchange (ETDEWEB)

    Herrera, Joshua M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-03-01

    This report is an analysis of the means of egress and life safety requirements for the laboratory building. The building is located at Sandia National Laboratories (SNL) in Albuquerque, NM. The report includes a prescriptive-based analysis as well as a performance-based analysis. Following the analysis are appendices which contain maps of the laboratory building used throughout the analysis. The top of all the maps is assumed to be north.

  8. Laboratory Building.

    Energy Technology Data Exchange (ETDEWEB)

    Herrera, Joshua M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-03-01

    This report is an analysis of the means of egress and life safety requirements for the laboratory building. The building is located at Sandia National Laboratories (SNL) in Albuquerque, NM. The report includes a prescriptive-based analysis as well as a performance-based analysis. Following the analysis are appendices which contain maps of the laboratory building used throughout the analysis. The top of all the maps is assumed to be north.

  9. Dynamics Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Dynamics Lab replicates vibration environments for every Navy platform. Testing performed includes: Flight Clearance, Component Improvement, Qualification, Life...

  10. Psychology Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — This facility provides testing stations for computer-based assessment of cognitive and behavioral Warfighter performance. This 500 square foot configurable space can...

  11. Propulsion Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Propulsion Lab simulates field test conditions in a controlled environment, using standardized or customized test procedures. The Propulsion Lab's 11 cells can...

  12. Chemistry Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Purpose: To conduct fundamental studies of highway materials aimed at understanding both failure mechanisms and superior performance. New standard test methods are...

  13. Analytical Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Analytical Labspecializes in Oil and Hydraulic Fluid Analysis, Identification of Unknown Materials, Engineering Investigations, Qualification Testing (to support...

  14. Identifying the Most Sensitive and Specific Sign and Symptom Combinations for Cholera: Results from an Analysis of Laboratory-Based Surveillance Data from Haiti, 2012–2013

    Science.gov (United States)

    Lucien, Mentor Ali Ber; Schaad, Nicolas; Steenland, Maria W.; Mintz, Eric D.; Emmanuel, Rossignol; Freeman, Nicole; Boncy, Jacques; Adrien, Paul; Joseph, Gerard A.; Katz, Mark A.

    2015-01-01

    Since October 2010, over 700,000 cholera cases have been reported in Haiti. We used data from laboratory-based surveillance for diarrhea in Haiti to evaluate the sensitivity, specificity, and positive (PPV) and negative predictive values (NPV) of the cholera case definitions recommended by the World Health Organization (WHO). From April 2012 to May 2013, we tested 1,878 samples from hospitalized patients with acute watery diarrhea; 1,178 (62.7%) yielded Vibrio cholerae O1. The sensitivity and specificity of the WHO case definition for cholera in an epidemi