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Sample records for abies grandis cdna

  1. Dynamics of frost resistance in various provenances of Abies grandis Lindl.

    Directory of Open Access Journals (Sweden)

    Adam Dolnicki

    2014-01-01

    Full Text Available The frost resistance of one year old needles and shoots of Abies grandis (seven provenances and Abies alba (one provenance was determined using Dexter's method since early autumn till late spring (September-May. Material was collected from trees over ten year old, planted in Beskid Sądecki Range near Krynica, 800 m above sea level. In this study it was found, that in early autumn (September A. grandis develops weaker frost hardiness than Abies alba. In the late autumn and in winter, provenances coming from region II (according to Müller, and especially provenance 12016, are equal in respect to frost hardiness of shoots and needles to Abies alba. Among the provenances from Müller's region I exceptional frost hardiness is displayed by provenance 12005, which is also characterized by fast growth. In spring, Abies alba flushes earlier than A. grandis, which makes it more susceptible to late frosts.

  2. Secondary resin production increases with vigor of Abies grandis inoculated with Trichosporium symbioticum in northeastern Oregon.

    Science.gov (United States)

    Gregory M. Filip; Erik Christiansen; Catherine A. Parks

    1989-01-01

    Thirty grand fir (Abies grandis (Dougl. ex D. Don) Lindl.) trees were artificially inoculated with the fungal symbiont, Trichosporium symbioticum Wright, to simulate attack by the fir engraver beetle, Scolytus ventralis LeConte. Fifteen months after treatment, secondary resin production, necrotic lesion...

  3. Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme.

    Science.gov (United States)

    Peters, R J; Flory, J E; Jetter, R; Ravn, M M; Lee, H J; Coates, R M; Croteau, R B

    2000-12-19

    The oleoresin secreted by grand fir (Abies grandis) is composed of resin acids derived largely from the abietane family of diterpene olefins as precursors which undergo subsequent oxidation of the C18-methyl group to a carboxyl function, for example, in the conversion of abieta-7,13-diene to abietic acid. A cDNA encoding abietadiene synthase has been isolated from grand fir and the heterologously expressed bifunctional enzyme shown to catalyze both the protonation-initiated cyclization of geranylgeranyl diphosphate to the intermediate (+)-copalyl diphosphate and the ionization-dependent cyclization of (+)-copalyl diphosphate, via a pimarenyl intermediate, to the olefin end products. Abietadiene synthase is translated as a preprotein bearing an N-terminal plastidial targeting sequence, and this form of the recombinant protein expressed in Escherichia coli proved to be unsuitable for detailed structure-function studies. Since the transit peptide-mature protein cleavage site could not be determined directly, a truncation series was constructed to delete the targeting sequence and prepare a "pseudomature" form of the enzyme that resembled the native abietadiene synthase in kinetic properties. Both the native synthase and the pseudomature synthase having 84 residues deleted from the preprotein converted geranylgeranyl diphosphate and the intermediate (+)-copalyl diphosphate to a nearly equal mixture of abietadiene, levopimaradiene, and neoabietadiene, as well as to three minor products, indicating that this single enzyme accounts for production of all of the resin acid precursors of grand fir. Kinetic evaluation of abietadiene synthase with geranylgeranyl diphosphate and (+)-copalyl diphosphate provided evidence for two functionally distinct active sites, the first for the cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate and the second for the cyclization of (+)-copalyl diphosphate to diterpene end products, and demonstrated that the rate

  4. Red de coexpresión de 320 genes de Tectona grandis Relacionados con procesos de estrés abiótico y xilogénesis

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    Vladimir Camel

    2017-01-01

    Full Text Available Tectona grandis es un árbol maderable de importancia económica en bosques tropicales y subtropicales. Mediante este estudio, se identificaron familias de factores de transcripción (FTs y genes codificantes para enzima, diferencialmente expresados en el xilema del tallo, implicados en la regulación de la respuesta a estrés abiótico y xilogénesis en T. grandis . Así, fue analizada la distribución evolutiva de 19 genes codificantes para FTs de T. grandis mediante análisis filogenéticos. También, fue utilizada la minería de bases de datos y publicaciones para identificar 320 genes de Arabidopsis thaliana (ortólogos a T. grandis como soporte experimental y predictivo. Como resultados, se encontraron FTs de las familias bZIP, MYB, NAC, ER, b HLH , NuY y genes que codifican enzimas. Así mismo, se logró analizar el interactoma de T. grandis encontrando correlaciones de Pearson significativas para genes que regulan vías metabólicas de fenilpropanoides y estrés abiótico. Además, la red de coexpresión reveló nodos y aristas entre los genes TgRAP1, TgMyB1, TgHSF1, TgMyB3, TgNAC1, TgTsiid1, TgLieTFs1, TgNuy3, TgRAP2 y TgNuy4 . En particular, los análisis de ontología génica mostraron 31 genes de respuesta a estrés abiótico, principalmente TgHShT1, TgHSF1 y TgHSF2 como correguladores. Además, se encontró que el regulador maestro TgNAC1 , está involucrado en la corregulación de otros factores de transcripción.

  5. Cloning, sequencing and expression of cDNA encoding growth ...

    Indian Academy of Sciences (India)

    Unknown

    317. 2.4 cDNA sequencing and analysis. The nucleotide sequence of the cloned H. fossilis GH. cDNA was determined by Sanger's dideoxy chain termi- nation method, using Perkin Elmer bigdye terminator kit in an ABI Prism 377 automated DNA sequencer. All other computational analysis of the GH cDNA was done using.

  6. Hea õpetamise grandi saajad

    Index Scriptorium Estoniae

    2016-01-01

    Õpetamise uurimist alustavad grandi toel Andrus Org, Riina Oruaas, Natalja Zagura (humanitaar- ja kunstide valdkond), Stefano Braghiroli, Aet Kiisla, Tiiu Taur (sotsiaalteadused), Marje Oona, Oivi Uibo, Daisy Volmer (meditsiinitead.), Svetlana Ganina, Natalja Lepik ja Raivo Raid (loodus- ja täppistead.)

  7. (TECTONA GRANDIS LEAF POWDER

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    Yash Mishra

    2015-01-01

    Full Text Available In this study, the adsorption potential of Teak (Tectona grandis leaf powder (TLP toremove Methylene blue (MB and Malachite Green (MG dye molecules from aqueoussolution was investigated. Batch experiments were conducted to evaluate the influenceof operational parameters such as, pH (2−9, adsorbent dosage (1−7 g/L, contact time(15−150 minutes and initial dye concentration (20−120 mg/L at stirring speed of 150rpm for the adsorption of MB and MG on TLP. Maximum removal efficiency of 98.4%and 95.1% was achieved for MB and MG dye, respectively. The experimentalequilibrium data were analysed using Langmuir, Freundlich and Temkin isothermmodels and it was found that, it fitted well to the Freundlich isotherm model. Thesurface structure and morphology of the adsorbent was characterized using scanningelectron microscopy (SEM and the presence of functional groups and its interactionwith the dye molecules were analysed using Fourier transform infrared spectroscopy(FTIR. Based on the investigation, it has been demonstrated that the teak leaf powderhas good potential for effective adsorption of methylene blue and malachite green dye.

  8. Conociendo a Aby Warburg

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    Luis Eugenio Campos

    2014-04-01

    Full Text Available Aby Warburg es la oculta figura creadora de los estudios de iconología. Personaje indiscutido, ha sido retomado en los últimos años a partir de la reedición de sus obras y de numerosos estudios que posicionan su figura intelectual y los impactos que tuvo su método de aproximación a las imágenes, específicamente aquellos que hablaban del renacimiento del paganismo. Conceptos como pathosformeln, sobrevivencias de la antigüedad, accesorios en movimiento y el análisis histórico, psicológico y contextual de la producción cultural de las imágenes, son hoy característicos de una todavía novedosa manera de entender el estudio de la iconología.

  9. The genome of Eucalyptus grandis

    Energy Technology Data Exchange (ETDEWEB)

    Myburg, Alexander A.; Grattapaglia, Dario; Tuskan, Gerald A.; Hellsten, Uffe; Hayes, Richard D.; Grimwood, Jane; Jenkins, Jerry; Lindquist, Erika; Tice, Hope; Bauer, Diane; Goodstein, David M.; Dubchak, Inna; Poliakov, Alexandre; Mizrachi, Eshchar; Kullan, Anand R. K.; Hussey, Steven G.; Pinard, Desre; van der Merwe, Karen; Singh, Pooja; van Jaarsveld, Ida; Silva-Junior, Orzenil B.; Togawa, Roberto C.; Pappas, Marilia R.; Faria, Danielle A.; Sansaloni, Carolina P.; Petroli, Cesar D.; Yang, Xiaohan; Ranjan, Priya; Tschaplinski, Timothy J.; Ye, Chu-Yu; Li, Ting; Sterck, Lieven; Vanneste, Kevin; Murat, Florent; Soler, Marçal; Clemente, Hélène San; Saidi, Naijib; Cassan-Wang, Hua; Dunand, Christophe; Hefer, Charles A.; Bornberg-Bauer, Erich; Kersting, Anna R.; Vining, Kelly; Amarasinghe, Vindhya; Ranik, Martin; Naithani, Sushma; Elser, Justin; Boyd, Alexander E.; Liston, Aaron; Spatafora, Joseph W.; Dharmwardhana, Palitha; Raja, Rajani; Sullivan, Christopher; Romanel, Elisson; Alves-Ferreira, Marcio; Külheim, Carsten; Foley, William; Carocha, Victor; Paiva, Jorge; Kudrna, David; Brommonschenkel, Sergio H.; Pasquali, Giancarlo; Byrne, Margaret; Rigault, Philippe; Tibbits, Josquin; Spokevicius, Antanas; Jones, Rebecca C.; Steane, Dorothy A.; Vaillancourt, René E.; Potts, Brad M.; Joubert, Fourie; Barry, Kerrie; Pappas, Georgios J.; Strauss, Steven H.; Jaiswal, Pankaj; Grima-Pettenati, Jacqueline; Salse, Jérôme; Van de Peer, Yves; Rokhsar, Daniel S.; Schmutz, Jeremy

    2014-06-11

    Eucalypts are the world s most widely planted hardwood trees. Their broad adaptability, rich species diversity, fast growth and superior multipurpose wood, have made them a global renewable resource of fiber and energy that mitigates human pressures on natural forests. We sequenced and assembled >94% of the 640 Mbp genome of Eucalyptus grandis into its 11 chromosomes. A set of 36,376 protein coding genes were predicted revealing that 34% occur in tandem duplications, the largest proportion found thus far in any plant genome. Eucalypts also show the highest diversity of genes for plant specialized metabolism that act as chemical defence against biotic agents and provide unique pharmaceutical oils. Resequencing of a set of inbred tree genomes revealed regions of strongly conserved heterozygosity, likely hotspots of inbreeding depression. The resequenced genome of the sister species E. globulus underscored the high inter-specific genome colinearity despite substantial genome size variation in the genus. The genome of E. grandis is the first reference for the early diverging Rosid order Myrtales and is placed here basal to the Eurosids. This resource expands knowledge on the unique biology of large woody perennials and provides a powerful tool to accelerate comparative biology, breeding and biotechnology.

  10. Starvation-induced morphological responses of the boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae)

    Science.gov (United States)

    Status of the boll weevil, Anthonomus grandis grandis Boheman, as a pest of cotton (Gossypium spp.) in the United States has diminished because of progress by eradication programs. However, this pest remains of critical importance in South America, and intractable populations in extreme South Texas ...

  11. Potential of AbiS as defence mechanism determined by conductivity measurement

    DEFF Research Database (Denmark)

    Holubová, Jitka; Josephsen, Jytte

    2007-01-01

    Aim: To compare pH and conductivity used in the determination of growth in reconstituted skim milk (RSM), to determine whether the presence of one or two plasmids in Lactococcus lactis had any influence on growth, and whether AbiS improved bacteriophages resistance of L. lactis. Methods and Results...... either AbiS1 or the restriction modification system LlaBIII was present. Conclusions: The earliest detection of growth was observed by measuring pH, rather than conductance. The plasmid-encoded AbiS system has a potential to be used as a phage resistance mechanisms in L. lactis during milk fermentations...

  12. Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1).

    Science.gov (United States)

    Ro, Dae-Kyun; Bohlmann, Jörg

    2006-08-01

    Diterpene resin acids are prominent defense compounds against insect pests and pathogens in conifers. Biochemical and molecular analyses in grand fir (Abies grandis), Norway spruce (Picea abies), and loblolly pine (Pinus taeda) have identified two classes of genes and enzymes that generate much of the structural diversity of terpenoid defense compounds: The terpenoid synthases (TPS) and cytochrome P450 monooxgenases (P450). Using a single substrate, geranylgeranyl diphosphate, families of single-product and multi-product diterpene synthases generate an array of cyclic diterpene olefins. These diterpenes are converted to diterpene resin acids by activity of one or more P450 enzymes. A few conifer diterpene synthases have previously been cloned and characterized in grand fir and in Norway spruce. We have also previously shown that the loblolly pine P450 abietadienol/abietadienal oxidase (PtAO) catalyzes multiple oxidations of several diterpene alcohols and aldehydes. Conifer diterpene synthases are thought to function in plastids while P450s can also be localized to plastids or to the endoplasmic reticulum (ER). Here, we show that a loblolly pine cDNA (PtTPS-LAS) encodes a typical multi-product conifer diterpene synthase that forms levopimaradiene, abietadiene, palustradiene, and neoabietadiene similar to the grand fir abietadiene synthase and Norway spruce levopimaradiene/abietadiene synthase. Subcellular targeting of PtTPS-LAS and PtAO to plastids and ER, respectively, was shown with green fluorescent fusion protein expression in tobacco cells. These data suggest that enzymes for conifer diterpene resin acid biosynthesis are localized to at least two different subcellular compartments, plastids and ER, requiring efficient transport of intermediates and secretion of diterpene resin acids into the extracelluar space.

  13. Micropropagation of Eucalyptus grandis and nitens using tissue culture techniques

    Energy Technology Data Exchange (ETDEWEB)

    Furze, M.J.; Creswell, C.F.

    1985-01-01

    Experiments with nodal explants of E. grandis and E. nitens seedlings and E. grandis coppice shoots showed that a large number of plants can be produced from a single explant using micropropagation. The percentage of micropropagated shoots that formed roots was about 90% for E. grandis and 80% for E. nitens. For both species, about 90% of the rooted shoots survived after hardening off. 9 references.

  14. Regeneration and transformation of Eucalyptus grandis

    OpenAIRE

    Esteki, Leila

    2013-01-01

    Dissertação de mestrado em Biologia Molecular, Biotecnologia e Bioempreendedorismo em Plantas Eucalyptus grandis is the most widely used species in planted forests in tropical and subtropical areas. The traits of interest underlying Eucalyptus breeding programs concern productivity and wood quality for the pulp and paper industry, as well as biotic and abiotic stress tolerance. The development of an efficient transformation protocol is necessary to explore eucalypt resources th...

  15. Pharmacological activity of Abies pindrow.

    Science.gov (United States)

    Singh, R K; Bhattacharya, S K; Acharya, S B

    2000-11-01

    The widely known tree Abies pindrow (Talisapatra) (family: Pinaceae), famous for its diverse clinical uses in Ayurvedic medicines, was investigated to rationalise some of the ancient claims. The petroleum ether (PE), benzene (BE), chloroform (CE), acetone (AE) and ethanol (EE) extracts of A. pindrow leaf were found to have mast cell stabilizing action in rats. The EE, AE and BE extracts offered bronchoprotection against histamine challenge in guinea-pigs. The BE, CE and PE extracts had protective role in aspirin-induced ulcer in rats. The results suggest that while terpenoids, flavonoids, glycosides and steroids are involved in mast cell protection, terpenoids and flavonoids are brochoprotective against histamine-induced bronchospasm. The ulcer protective action of PE, BE and CE fractions of A. pindrow may be attributable to steroids contents only because though all the extracts tested positive for glycosides, the extracts EE and AE did not have any ulcer protective role.

  16. Normalizing cDNA libraries.

    Science.gov (United States)

    Bogdanov, Ekaterina A; Shagina, Irina; Barsova, Ekaterina V; Kelmanson, Ilya; Shagin, Dmitry A; Lukyanov, Sergey A

    2010-04-01

    The characterization of rare messages in cDNA libraries is complicated by the substantial variations that exist in the abundance levels of different transcripts in cells and tissues. The equalization (normalization) of cDNA is a helpful approach for decreasing the prevalence of abundant transcripts, thereby facilitating the assessment of rare transcripts. This unit provides a method for duplex-specific nuclease (DSN)-based normalization, which allows for the fast and reliable equalization of cDNA, thereby facilitating the generation of normalized, full-length-enriched cDNA libraries, and enabling efficient RNA analyses. (c) 2010 by John Wiley & Sons, Inc.

  17. Growth and yield models for Eucalyptus grandis grown in Swaziland ...

    African Journals Online (AJOL)

    The aim of this study was to develop a stand-level growth and yield model for short-rotationEucalyptus grandis grown for pulp wood production at Piggs Peak in Swaziland. The data were derived from a Nelder 1a spacing trial established with E. grandis clonal cuttings in 1998 and terminated in 2005. Planting density ...

  18. Clonal differences in log end splitting in Eucalyptus grandis in ...

    African Journals Online (AJOL)

    This paper discusses the juvenile–mature correlation of log end splitting among Eucalyptus grandis clones from two trials and how differences in splitting relate to differences in wood density, pith-to-bark gradient and growth rate. Two approximately 20-year-old Eucalyptus grandis clonal trials at Bergvliet plantation were ...

  19. cDNA library preparation.

    Science.gov (United States)

    Kooiker, Maarten; Xue, Gang-Ping

    2014-01-01

    The construction of full-length cDNA libraries allows researchers to study gene expression and protein interactions and undertake gene discovery. Recent improvements allow the construction of high-quality cDNA libraries, with small amounts of mRNA. In parallel, these improvements allow for the incorporation of adapters into the cDNA, both at the 5' and 3' end of the cDNA. The 3' adapter is attached to the oligo-dT primer that is used by the reverse transcriptase, whereas the 5' adapter is incorporated by the template switching properties of the MMLV reverse transcriptase. This allows directional cloning and eliminates inefficient steps like adapter ligation, phosphorylation, and methylation. Another important step in the construction of high-quality cDNA libraries is the normalization. The difference in the levels of expression between genes might be several orders of magnitude. Therefore, it is essential that the cDNA library is normalized. With a recently discovered enzyme, duplex-specific nuclease, it is possible to normalize the cDNA library, based on the fact that more abundant molecules are more likely to reanneal after denaturation compared to rare molecules.

  20. Normalized cDNA libraries

    Science.gov (United States)

    Soares, Marcelo B.; Efstratiadis, Argiris

    1997-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  1. Morphology of immatures of Euschistus (Mitripus grandis (Insecta: Hemiptera: Pentatomidae

    Directory of Open Access Journals (Sweden)

    Larissa Donida Biasotto

    2013-06-01

    Full Text Available Immatures are important sources of information about characters, thus potentially improving classification and phylogenies. Euschistus (Mitripus grandis Rolston, 1978 occurs in southern and southeastern Brazil. There are no references in the literature about this species other than the original description and distribution records. Our aim is to describe the egg and five nymphal instars of E. (M. grandis, addressing the ontogeny of the external scent efferent system. Specimens of E. (M. grandis were collected on Solanum pseudocapsicum Linnaeus (Solanaceae in São Francisco de Paula, Rio Grande do Sul, Brazil, and reared in the laboratory. The egg of E. (M. grandis is white and barrel-shaped, chorion translucent bearing spines connected by thin sheets forming polygons. Aero-mycropilar processes translucent and slightly clubbed. The nymphs bear anterior dorsal abdominal scent glands (DAG with slit-like ostiole continued by a short groove; from the second instar onward median and posterior DAGs with rounded ostiole bearing closing cuticular valve. Spout peritreme flat and curved anteriorly, evaporatorium network-shaped surrounding ostiole plus peritrema; alveoli surface with trabeculae. Nymphs of E. (M. grandis can be distinguished from other species of Euschistus by the color pattern and morphological features. The most striking morphological characteristic of 4th and 5th instar nymphs of E. (M. grandis is the broad, deflected projection of humeral angles.

  2. Sargent's fir hybrid: Abies amabilis x lasicarpa

    Science.gov (United States)

    William B. Critchfield

    1977-01-01

    On a short trip into the northern Olympic Mountains of Washington in the summer of 1896, Professor Charles Sprague Sargent found a fir tree that he thought might be a natural hybrid between Abies amabilis (Dougl.) Forbes and A. lasiocarpa (Hook.) Nutt. The founder and Director of the Arnold Arboretum, Sargent was generally...

  3. Interviewing Children with Acquired Brain Injury (ABI)

    Science.gov (United States)

    Boylan, Anne-Marie; Linden, Mark; Alderdice, Fiona

    2009-01-01

    Research into the lives of children with acquired brain injury (ABI) often neglects to incorporate children as participants, preferring to obtain the opinions of the adult carer (e.g. McKinlay et al., 2002). There has been a concerted attempt to move away from this position by those working in children's research with current etiquette…

  4. Zeuxine grandis Seidenf. (Orchidaceae - an Addition to the Orchid Flora of India

    Directory of Open Access Journals (Sweden)

    Avishek Bhattacharjee

    2010-09-01

    Full Text Available Zeuxine grandis Seidenf., is being reported for the first time from India. We provide line drawing and color photo of specimen of Z. grandis in support of our treatment and to facilitate identification of the species.

  5. Development of height-volume relationships in second growth Abies grandis for use with aerial LiDAR

    Science.gov (United States)

    Wade T. Tinkham; Alistair M. S. Smith; David L. R. Affleck; Jarred D. Saralecos; Michael J. Falkowski; Chad M. Hoffman; Andrew T. Hudak; Michael A. Wulder

    2016-01-01

    Following typical forest inventory protocols, individual tree volume estimates are generally derived via diameter-at-breast-height (DBH)-based allometry. Although effective, measurement of DBH is time consuming and potentially a costly element in forest inventories. The capacity of airborne light detection and ranging (LiDAR) to provide individual tree-level...

  6. Embryogenic culture initiation and somatic embryo development in hybrid firs (Abies alba x Abies cephalonica, and Abies alba x Abies numidica).

    Science.gov (United States)

    Salajova, T; Jasik, J; Kormutak, A; Salaj, J; Hakman, I

    1996-03-01

    Embryogenic cultures were established from silver fir (Abies alba Mill.) female megagametliophytes with developing embryos and from excised mature embryos after pollination with Abies cephalonica Lond. or Abies numidica DeLann pollea The frequency of embryogenic callus formation was dependent on genotype, collection time, medium and explants used. The embryogenic callus initiation potential of megagamethophytes with developing embryos in both hybrids was higher in early July and dropped as the zygotic embryos matured. Excised cotyledonary embryos were less suitable for induction of embryogenic cultures. SH medium supplemented with 1mg/l BAP was the most efficient for callus induction and maintenance. Cultures were composed of early somatic embryos with an embryonal mass formed of highly cytoplasmic cells, rich in cell organelles and a suspensor built up by vacuolated, strongly elongated cells. Maturation of embryos was detected with the formation of bipolar structures with shoot and root apices. Nutrition reserves were observed in cells of embryos cultured on DCR medium containing 1 or 10 mg/l ABA. Cotyledon formation, hypocotyl elongation and low frequency germination occured following transfer of the embryos to the same medium without ABA.

  7. Seed viability constants for Eucalyptus grandis Constantes de viabilidade para sementes de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Jussara Bertho Fantinatti

    2007-01-01

    Full Text Available This work aimed to analyse Eucalyptus grandis W.Hill ex Maiden seed behaviour, under controlled deterioration, and to estimate viability equation constants for the species. Seeds were harvested in the growing season of 1999, and the moisture contents were adjusted from 11.3% to a range between 1.2 and 18.1% at 25ºC. The subsamples were sealed into laminate aluminium-foil packets, for storage in incubators at 40, 50 and 65±0.5ºC. The seeds presented orthodox performance, in which the constants for predicting seed longevity of E. grandis were K E = 9.661, C W = 6.467, C H = 0.03498 and C Q = 0.0002330. The usual and inverse relationship between water content and seed longevity was also observed. The lowest moisture content limit for application of the viability equation at 65ºC was 4.9%, estimated under hygroscopic equilibrium with 23% of relative humidity in the storage environment.Este trabalho teve como objetivos verificar o desempenho de sementes de Eucalyptus grandis W.Hill ex Maiden, após a deterioração em condições controladas, e obter as constantes da equação de viabilidade. As sementes foram colhidas na safra de 1999, e a umidade foi ajustada de 11,3% para valores entre 1,2 e 18,1% a 25ºC As subamostras foram acondicionadas em embalagens de alumínio termossoldadas, armazenadas a 40, 50 e 65±0,5ºC. As sementes apresentaram um desempenho ortodoxo em relação ao armazenamento. As constantes para a predição da longevidade foram K E = 9,661, C W = 6,467, C H = 0,03498 e C Q = 0,0002330. Foi observada a relação inversa entre teor de água e longevidade. O limite inferior de grau de umidade, calculado para aplicação da equação a 65ºC, foi de 4,9%, estimativa obtida sob equilíbrio higroscópico com umidade relativa de 23% no ambiente de armazenamento.

  8. Stomatal characteristics of Eucalyptus grandis clonal hybrids in ...

    African Journals Online (AJOL)

    This study describes the stomatal response occurring during water stress and subsequent recovery of three Eucalyptus grandis clonal hybrids. The aim was to investigate the degree to which stomatal conductance (gs) and stomatal density differ between the clonal hybrids across seasons and in response to water stress.

  9. Water relations of Eucalyptus nitens x Eucalyptus grandis : is there ...

    African Journals Online (AJOL)

    Water relations of Eucalyptus nitens x Eucalyptus grandis : is there interclonal variation in response to experimentally imposed water stress? ... Southern Forests: a Journal of Forest Science ... However, water stress reduced shoot hydraulic conductance and stem hydraulic conductivity with significant interclonal effects.

  10. Realized gain from breeding Eucalyptus grandis in Florida

    Science.gov (United States)

    George Meskimen

    1983-01-01

    E. grandis is in the fourth generation of selection in southwest Florida. The breeding strategy combines a provenance trial, genetic base population, seedling seed orchard, and progeny test in a single plantation where all families are completely randomized in single-tree plots. That planting configuration closely predicted the magnitude of genetic...

  11. Simultaneous growth and yield models for Eucalyptus grandis (Hill ...

    African Journals Online (AJOL)

    Simultaneous stand-level growth and yield models for Eucalyptus grandis in Zimbabwe were developed from Correlated Curve Trend (CCT) and Nelder wheel experiments replicated on five different sites. Nonlinear three-stage least squares method was used to simultaneously fit prediction and projection equations for ...

  12. Cafe Grandis kõlab lautomuusika / Anneli Remme

    Index Scriptorium Estoniae

    Remme, Anneli, 1968-

    2008-01-01

    Corelli Musicu salongiõhtutel festivali "Fiesta de la Guitarra" raames esineb trio koosseisus Stewart McCoy (lautomängija), Robert Staak (lautomängija) ja Maria Staak (laulja), esinejatest. Esitatakse renessansiaja autorite lautoduette ja -laule. Kontsert 8. nov. Pärnu hotellis sarjas "Café Grandi muusikasalong"

  13. Biology of Anastrepha grandis (Diptera: Tephritidae) in Different Cucurbits.

    Science.gov (United States)

    Bolzan, Anderson; Nava, Dori E; Garcia, Flávio R M; Valgas, Ricardo A; Smaniotto, Giovani

    2015-06-01

    Anastrepha grandis (Macquart) (Diptera: Tephritidae) is one of the main pests of cucurbits in Brazil. Losses occur due to the damage caused to the fruits and the embargo on exports, as A. grandis is considered a quarantine pest in countries that import Brazilian cucurbits. This study aimed to evaluate the development of A. grandis in hosts of the Cucurbitaceae family. The hosts used were stem squash (Cucurbita pepo L.), squash (Cucurbita moschata Duchesne), chayote [Sechium edule (Jacq.) Swartz], mini watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai], Spanish melon (Cucumis melo L.), hybrid squash "Tetsukabuto" (C. moschata×Cucurbita maxima Duchesne), and salad cucumber (Cucumis sativus L.). We evaluated the viability and duration of egg-to-pupa period, pupal weight, sex ratio, and average number of pupae per fruit under controlled conditions of temperature, relative humidity, and photophase. The preoviposition and oviposition periods, fecundity, fertility, and longevity of females were determined for adults. Hosts of the genus Cucurbita provided a better development of A. grandis in comparison with other hosts, and presented a greater number of insects on fruit as well as higher infestation rate. Fecundity and longevity were also higher for females that developed in hosts of the genus Cucurbita, although values of these biological parameters varied between stem squash, squash, hybrid squash "Tetsukabuto." © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  14. Killing Eucalyptus grandis cut stumps after multiple coppice rotations ...

    African Journals Online (AJOL)

    isopropylamine salt, 192 g l-1) were applied to a basal frill, the cut surface, or the foliage of coppice regrowth of E. grandis stumps. ... The application of herbicides to a basal frill killed 90 % of the stumps and required the least follow-up control.

  15. Effects of Tectona grandis (teak) plantation on soil microorganisms ...

    African Journals Online (AJOL)

    Colonies of the microorganisms were examined, counted and identified with microscopes and biochemical tests. Results showed significant differences of micro-organisms counts (bacteria, P=0.01) between Tectona grandis plantation and natural forest while no significant difference was observed between the months of ...

  16. Diagnosing foliar nutrient dynamics of Eucalyptus grandis in ...

    African Journals Online (AJOL)

    Fertilisation is one of the most cost-effective methods of increasing and maintaining the productivity of Eucalyptus grandis plantations in South Africa. This silvicultural practice can be optimised by using the foliar nutrient ratios measured in plants at maximum growth as a guideline for fertiliser application. The foliar nutrient ...

  17. Shoot and root morphogenesis from Eucalyptus grandis x urophylla ...

    African Journals Online (AJOL)

    Eucalyptus grandis x urophylla plantlets were regenerated via indirect organogenesis. Histological assessment of their development focused on identifying the calli, the differentiation of shoots from the calli and the shoot-root junction from the nascent shoots. Vascular tissue formation within the callus preceded that of ...

  18. Soil carbon estimation from eucalyptus grandis using canopy spectra

    African Journals Online (AJOL)

    Mapping soil fertility parameters, such as soil carbon (C), is fundamentally important for forest management and research related to forest growth and climate change. This study seeks to establish the link between Eucalyptus grandis canopy spectra and soil carbon using raw and continuum-removed spectra. Canopy-level ...

  19. Genetic improvement of Eucalyptus grandis using breeding seedling ...

    African Journals Online (AJOL)

    Eucalyptus grandis is commercially important in Zimbabwe and a breeding program has been in progress since 1962. A classical breeding strategy was used initially but, in 1981, the Multiple Population Breeding Strategy (MPBS) was implemented and the concept of the Breeding Seedling Orchard (BSO) became central to ...

  20. Prospects of Tectona Grandis as a Feedstock for Biodiesel

    International Nuclear Information System (INIS)

    Sarin, Amit; Singh, Meetu; Sharma, Neerja; Singh, N. P.

    2017-01-01

    The limited availability of fossil fuels has encouraged the need of replacement fuels of renewable nature. Among the renewable fuels, biodiesel produced from oil seeds and food wastes has been favored by the majority of researchers. In this study, Tectona Grandis seed oil has been investigated as a non-edible feedstock for biodiesel. The oil content of seed is 43% which makes it suitable for commercial production of biodiesel. The synthesis of biodiesel from T. Grandis oil was done with transesterification reaction giving high percentage yield of biodiesel which reached to 89%. The T. Grandis biodiesel was subjected to determine various physicochemical parameters by standard testing methods and found in agreement with the ASTM D-6751 and EN-14214 standards. The fatty-acid methyl ester composition for the biodiesel is composed of 42.71% oleic acid, 13.1% palmitic acid, and 31.51% linoleic acid. The biodiesel showed low oxidation stability which is attributed to high percentage of unsaturation. To address this issue, synthetic antioxidants were added to increase its resistance towards oxidation. By considering all the parameters, the present study reveals that T. Grandis seed oil is reliable for the production of biodiesel with encouraging probability in future.

  1. Prospects of Tectona Grandis as a Feedstock for Biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Sarin, Amit, E-mail: amit.sarin@yahoo.com [Department of Physical Sciences, I.K. Gujral Punjab Technical University, Kapurthala (India); Singh, Meetu [Department of Applied Sciences, I.K. Gujral Punjab Technical University, Kapurthala (India); Sharma, Neerja [PG Department of Physics and Electronics, DAV College, Amritsar (India); Singh, N. P. [Department of Planning and External Development, I.K. Gujral Punjab Technical University, Kapurthala (India)

    2017-10-26

    The limited availability of fossil fuels has encouraged the need of replacement fuels of renewable nature. Among the renewable fuels, biodiesel produced from oil seeds and food wastes has been favored by the majority of researchers. In this study, Tectona Grandis seed oil has been investigated as a non-edible feedstock for biodiesel. The oil content of seed is 43% which makes it suitable for commercial production of biodiesel. The synthesis of biodiesel from T. Grandis oil was done with transesterification reaction giving high percentage yield of biodiesel which reached to 89%. The T. Grandis biodiesel was subjected to determine various physicochemical parameters by standard testing methods and found in agreement with the ASTM D-6751 and EN-14214 standards. The fatty-acid methyl ester composition for the biodiesel is composed of 42.71% oleic acid, 13.1% palmitic acid, and 31.51% linoleic acid. The biodiesel showed low oxidation stability which is attributed to high percentage of unsaturation. To address this issue, synthetic antioxidants were added to increase its resistance towards oxidation. By considering all the parameters, the present study reveals that T. Grandis seed oil is reliable for the production of biodiesel with encouraging probability in future.

  2. Assessment of wood density of seven clones of Eucalyptus grandis ...

    African Journals Online (AJOL)

    With the objective of evaluating the correlation of wood basic density with age in seven Eucalyptus grandis clones planted in Brazil, five trees in each clone were sampled at the ages of 0, 5, 1, 5, 2, 5, 3, 5, 4, 5 and 7, 5 years. The analysis of these samples showed that the intraclonal variation of the basic density (except for 0, ...

  3. The potential of young, green finger-jointed Eucalyptus grandis ...

    African Journals Online (AJOL)

    Drying will occur naturally while the lumber is fixed within the roof truss structure. The objectives of this study were (1) to investigate the strength and stiffness variation of the finger-jointed E. grandis product in both the green and dry state for different age and dimension lumber, (2) to investigate the variation in density, warp ...

  4. Dependence of rate of germination of teak ( Tectona grandis ) seeds ...

    African Journals Online (AJOL)

    A study was conducted to determine suitable sources of teak (Tectona grandis) seeds and methods of treating the seeds to promote higher rate of germination, with the objective to supply large quantities of seedlings for developing commercial teak plantations in Ghana. The field work involved seed collection, seed pericarp ...

  5. Antidiabetic properties of the methanolic extract of Bridelia grandis ...

    African Journals Online (AJOL)

    Jane

    2011-03-28

    Mar 28, 2011 ... Bridelia grandis is used in most parts of tropical Africa for the treatment of diabetes. The anti-diabetic properties of the methanolic stem bark extract of this plant was studied in ob/ob and db/db mice. The plant extract induced hypoglycaemic effects of long duration in the ob/ob mice, while its effects were.

  6. Annotation and Re-Sequencing of Genes from De Novo Transcriptome Assembly of Abies alba (Pinaceae

    Directory of Open Access Journals (Sweden)

    Anna M. Roschanski

    2013-01-01

    Full Text Available Premise of the study: We present a protocol for the annotation of transcriptome sequence data and the identification of candidate genes therein using the example of the nonmodel conifer Abies alba. Methods and Results: A normalized cDNA library was built from an A. alba seedling. The sequencing on a 454 platform yielded more than 1.5 million reads that were de novo assembled into 25 149 contigs. Two complementary approaches were applied to annotate gene fragments that code for (1 well-known proteins and (2 proteins that are potentially adaptively relevant. Primer development and testing yielded 88 amplicons that could successfully be resequenced from genomic DNA. Conclusions: The annotation workflow offers an efficient way to identify potential adaptively relevant genes from the large quantity of transcriptome sequence data. The primer set presented should be prioritized for single-nucleotide polymorphism detection in adaptively relevant genes in A. alba.

  7. Annotation and re-sequencing of genes from de novo transcriptome assembly of Abies alba (Pinaceae).

    Science.gov (United States)

    Roschanski, Anna M; Fady, Bruno; Ziegenhagen, Birgit; Liepelt, Sascha

    2013-01-01

    We present a protocol for the annotation of transcriptome sequence data and the identification of candidate genes therein using the example of the nonmodel conifer Abies alba. • A normalized cDNA library was built from an A. alba seedling. The sequencing on a 454 platform yielded more than 1.5 million reads that were de novo assembled into 25149 contigs. Two complementary approaches were applied to annotate gene fragments that code for (1) well-known proteins and (2) proteins that are potentially adaptively relevant. Primer development and testing yielded 88 amplicons that could successfully be resequenced from genomic DNA. • The annotation workflow offers an efficient way to identify potential adaptively relevant genes from the large quantity of transcriptome sequence data. The primer set presented should be prioritized for single-nucleotide polymorphism detection in adaptively relevant genes in A. alba.

  8. Antibacterial, antioxidant and cell proliferative properties of Coccinia grandis fruits

    Directory of Open Access Journals (Sweden)

    Prashant Sakharkar

    2017-06-01

    Full Text Available Objective: Little knowledge is available on the antimicrobial and antioxidant properties of Coccina grandis fruits and no study has reported on its cell proliferative property. The aim of this study was to examine the antimicrobial, antioxidant and cell proliferative property of fruits of C. grandis. Material and Methods: Fruits of C. grandis were extracted using water; ethanol and acetone by cold and hot Soxhlet extraction. The antibacterial activities of the extracts were tested against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa using the modified Kirby-Bauer diffusion method and compared against erythromycin. The antioxidant property was determined using Cayman's antioxidant assay; whereas cell proliferation/cytotoxic properties were evaluated using the Cell Titer 96 Aqueous One Solution Cell MTS assay with MDA-MB 321 breast cancer cells. Data were analyzed for correlation and differences using unpaired student's t-test and one-way ANOVA. A p value of Results: Both cold and hot ethanol and acetone extracts of C. grandis fruits showed some degree of bacterial growth inhibition. Acetone extracts exhibited higher antibacterial activity. Both ethanol extracts showed antioxidant property when compared with standard Trolox. In contrary to cytotoxicity, all four extracts showed cell proliferation compared to controls at different concentrations. However, acetone extracts exhibited greater cell proliferation compared to ethanol extracts and cold extracts performed better than the hot extracts. Conclusion: C. grandis fruits exhibited some degree of antimicrobial, antioxidant and cell proliferative properties. Further investigation is warranted to isolate, confirm and characterize phytochemicals that are responsible for the medicinal properties observed.

  9. Somatic Embryogenesis of Abies cephalonica Loud.

    Science.gov (United States)

    Krajňáková, Jana; Häggman, Hely

    2016-01-01

    Greek fir (Abies cephalonica Loudon) belongs to the Mediterranean fir species and is widely distributed in the mountains of Central and Southern Greece. Considering a climatic scenario, infestation by pathogens or insects and fire episodes, it has been proposed that Mediterranean firs could be in danger in some parts of their present range but, on the other hand, could also replace other species in more northern zones with temperate humid climates (e.g., silver fir, Abies alba Mill.). As fir species are generally highly productive and therefore important for commercial forestry, they have traditionally been involved in conventional tree improvement programs. A lot of effort has been put into the development of vegetative propagation methods for firs, in order to rapidly gain the benefits of traditional breeding to be utilized in reforestation. The present paper provides up to date information on protocols for somatic embryogenesis (i.e., the most promising in vitro method for vegetative propagation) of Greek fir. Moreover, the protocols for cryopreservation and long-term storage of embryogenic material are described as well.

  10. Comparison between oscillometric- and Doppler-ABI in elderly individuals

    Directory of Open Access Journals (Sweden)

    Takahashi I

    2013-03-01

    Full Text Available Ikuno Takahashi,1,3 Kyoji Furukawa,2 Waka Ohishi,1 Tetsuya Takahashi,3 Masayasu Matsumoto,3 Saeko Fujiwara11Department of Clinical Studies, 2Department of Statistics, Radiation Effects Research Foundation (RERF, Hiroshima, Japan; 3Department of Clinical Neuroscience and Therapeutics, Hiroshima University, Hiroshima, JapanAbstract: Peripheral arterial disease (PAD generally remains under-recognized, mainly due to the specialized technical skills required to detect the low values of the ankle-brachial index (ABI. As a simpler and faster alternative to the standard method using continuous-wave Doppler ultrasound, we evaluated automated oscillometric ABI measurement by VP-2000 with an elderly cohort of 113 subjects (age range, 61 to 88 years. The standard deviation in ABIs measured by the Doppler method was statistically greater than that measured by the oscillometric method for each of the two legs (P < 0.001. Correlations in ABIs between the two methods were 0.46 for the left leg and 0.61 for the right leg; this result appears to have been caused by interobserver variation in the Doppler ABI measurements. While the trend showing greater differences between average oscillometric- and Doppler-ABIs was significant at the lower ABI ranges, there was little indication of differences in measurements having an average ABI > 1.1. The difference between the methods was suggestively larger in subjects who were smokers than in non-smokers (P = 0.09, but the difference was not affected by other potential atherosclerotic risk factors, including age at examination (P > 0.50. A larger difference at lower ABIs led to better PAD detection by the Doppler method compared to the oscillometric method (sensitivity = 50%, specificity = 100%, although the overall agreement was not small (Cohen's Kappa = 0.65. Our findings indicate that oscillometric devices can provide more accurate estimation of the prevalence of PAD in elderly individuals than the conventional Doppler

  11. A book on Silver fir (Abies alba in Romania

    Directory of Open Access Journals (Sweden)

    Mercurio R

    2005-01-01

    Full Text Available The contents of a new book on Silver fir (Abies alba in Romania is presented, which describes with special emphasis the characteristics of Silver fir forest in Southern Carpathians

  12. Long-Term Effects of Exotic Tree Species ( Tectona grandis Linn. F ...

    African Journals Online (AJOL)

    Long-Term Effects of Exotic Tree Species ( Tectona grandis Linn. F.) on the Status of Extractable Micronutrients in the ... The study therefore implied that Tectona grandis has an extractive property on micronutrient particularly on soils that are low in these nutrients. Nigerian Journal of Soil and Environmental Research Vol.

  13. In vitro antioxidant activities of the fractions of Coccinia grandis l. leaf ...

    African Journals Online (AJOL)

    ... flavonoid compounds present in the fractions. The results obtained in the present study indicate that the leaves of C. grandis are a potential source of natural antioxidant. Keywords:Coccinia grandis (Cucurbitaceae), free radicals, antioxidant, pro-oxidant. African Journal of Traditional and Complementary Medicine Vol.

  14. [Study on heavy metals in growing area soils and young fruit of Citrus grandis 'Tomentosa'].

    Science.gov (United States)

    Zhong, Jihongi; Lin, Lanwen; Tan, Jun; Lin, Li; Huang, Lanzhen; Liao, Guanrong

    2005-05-01

    To determine the status of heavy metals contamination in growing area soils and young fruits of Citrus grandis. Application of Nemerow's index for heavy metals assessment of soil and young fruit of Citrus grandis, as well as according to national environmental quality standard for soils and green trade standards of importing & exporting medicinal plants & preparations. The heavy metals content of most of the growing area soils of Citrus grandis rates as first, and of the rest ones rates as second. The heavy metals content in young fruits of Citrus grandis is within the limitation of quantity of criteria. The environmental quality of growing area soils of Citrus grandis in Huazhou City is totally in good.

  15. ECONOMIC ROTATION OF Eucalyptus grandis PLANTATIONS FOR PULP PRODUCTION

    Directory of Open Access Journals (Sweden)

    Thais Cunha Ferreira

    2004-07-01

    Full Text Available The objectives of the research were: to determine the economic impact of several minimum diameter and length of logs in economic rotation age, economic feasibility of Eucalyptus grandis plantation for cellulose production; to determine the economic loss of cutting the stand before or after the optimal economic rotation age. A biometric model for making wood volume prognosis was developed using data of a trial of Eucalyptus grandis plantation envisaging pulp production. Eucalyptus grandis stands of 19 and 103 months old, in the spacing 3 x 2 and 3 x 3 m in site index of 30; 28; 26 and 24 m were used. Theprognosis started at the age zero, considering logs of 2.5; 2.8; 4.0 and 6.0 m of length for minimum diameter varying from 4 to 10 cm, in intervals of 2 cm. Net Present Worth (VPL was used the economic criterion, considering an infinite horizon and a cost relation including reestablishment, yearly maintenance, logging and wood transportation costs. The main conclusions were: increases in the minimum diameter and or in logs length increase the rotation age; harvesting the stands in ages different from the optimal one cause large economic loss mainly in the better sites; the economic loss is larger if the harvest is made before the optimal economic rotation than if it is make after; economic feasibility increases when the minimum diameter is smaller and when the length of the logs is shorter. Any way, before making any decision it is necessary to take into account possible technical restrictions and effect on harvest and transportation costs caused by changer in the length of logs and in the size of the minimum commercial diameter.

  16. Grandi Eventi: indicatori di classificazione e incidenza sui sistemi urbani

    Directory of Open Access Journals (Sweden)

    Giuseppe Mazzeo

    2008-08-01

    Full Text Available I grandi eventi sono ritenuti una opportunità per le città in quanto sono uno straordinario catalizzatore di investimenti mirati alla trasformazione della città. L’azione dei grandi eventi non è confinata solo nel periodo di svolgimento dell’evento stesso ma si estende ad un periodo molto più ampio, prima e, soprattutto, dopo il suo termine. Nell’organizzazione delle manifestazioni più recenti le trasformazioni urbane hanno interessato in modo sempre maggiore parti già in precedenza urbanizzate, al punto che l’evento diviene l’occasione per trasformare la città costruita e per modificarne il profilo. Il paper approfondisce l’argomento della classificazione degli eventi e del loro impatto sul sistema urbano sviluppandosi in tre parti. Nella prima si analizzano due tipologie di grandi eventi (le esposizioni internazionali e i giochi olimpici con l’obiettivo di pervenire ad una definizione condivisa. Nella seconda parte si identificano i più importanti fattori ed indicatori per l’analisi di questa specifica categoria di avvenimenti. Infine, nella terza parte, si approfondiscono i fattori connessi all’impatto sul sistema urbano e all’organizzazione della sua mobilità. In particolare, viene approfondito il concetto di “effetto pulsar”, ossia gli effetti moltiplicativi sulla evoluzione urbana dipendenti dalla organizzazione di eventi multipli in parallelo o in sequenza. Uno degli elementi più negativi connessi alla organizzazione di un grande evento è la dispersione nel tempo dei benefici acquisiti grazie ad esso; per evitare ciò è necessario che l’evento sia seguito da politiche strategiche continue in modo da preservare i vantaggi acquisiti.

  17. New naphthalene derivative from the leaves of Cassia grandis L.

    Science.gov (United States)

    Trinh, Pham Thi Nhat; Luan, Ngoc Quoc; Tri, Mai Dinh; Khanh, Vu Duy; An, Nguyen Huu; Minh, Phan Nhat; An, Pham Ngoc; Thuy, Nguyen Thi Le; Phung, Nguyen Kim Phi; Dung, Le Tien

    2017-08-01

    Cassia grandis is used for the treatment of skin disorders, back pain, aches, etc. in Vietnamese traditional medicine. In this paper, phytochemicals of the leaves were investigated. A new naphthalene derivative (1, cassgranon D) and, seven known compounds rutin (2), afzelin (3), quercitrin (4), epicatechin (5), (-)-epiafzelechin (6), isoquercitrin (7) and aloe emodin (8) were isolated from the ethyl acetate and methanol extracts. Their structures were elucidated by spectral evidences (UV, IR, MS, 1 H, 13 C, DEPT, HSQC and HMBC NMR), as well as by comparing with published data.

  18. Annotation and re-sequencing of genes from de novo transcriptome assembly of Abies alba (Pinaceae)1

    Science.gov (United States)

    Roschanski, Anna M.; Fady, Bruno; Ziegenhagen, Birgit; Liepelt, Sascha

    2013-01-01

    • Premise of the study: We present a protocol for the annotation of transcriptome sequence data and the identification of candidate genes therein using the example of the nonmodel conifer Abies alba. • Methods and Results: A normalized cDNA library was built from an A. alba seedling. The sequencing on a 454 platform yielded more than 1.5 million reads that were de novo assembled into 25149 contigs. Two complementary approaches were applied to annotate gene fragments that code for (1) well-known proteins and (2) proteins that are potentially adaptively relevant. Primer development and testing yielded 88 amplicons that could successfully be resequenced from genomic DNA. • Conclusions: The annotation workflow offers an efficient way to identify potential adaptively relevant genes from the large quantity of transcriptome sequence data. The primer set presented should be prioritized for single-nucleotide polymorphism detection in adaptively relevant genes in A. alba. PMID:25202477

  19. An efficient procedure to stably introduce genes into an economically important pulp tree (Eucalyptus grandis x Eucalyptus urophylla).

    Science.gov (United States)

    Tournier, Vincent; Grat, Sabine; Marque, Christiane; El Kayal, Walid; Penchel, Ricardo; de Andrade, Gisele; Boudet, Alain-Michel; Teulières, Chantal

    2003-08-01

    Regeneration problems are one of the main limitations preventing the wider application of genetic engineering strategies to the genus Eucalyptus. Seedlings from Eucalyptus grandis x Eucalyptus urophylla were selected according to their regeneration (adventitious organogenesis) and transformation capacity. After in vitro cloning, the best genotype of 250 tested was transformed via Agrobacterium tumefaciens. A cinnamyl alcohol dehydrogenase (CAD) antisense cDNA from Eucalyptus gunnii was transferred, under the control of the 35S CaMV promoter with a double enhancer sequence, into a selected genotype. According to kanamycin resistance and PCR verification, 120 transformants were generated. 58% were significantly inhibited for CAD activity, and nine exhibited the highest down-regulation, ranging from 69 to 78% (22% residual activity). Southern blot hybridisation showed a low transgene copy number, ranging from 1 to 4, depending on the transgenic line. Northern analyses on the 5-16 and 3-23 lines (respectively one and two insertion sites) demonstrated the antisense origin of CAD gene inhibition. With respectively 26 and 22% of residual CAD activity, these two lines were considered as the most interesting and transferred to the greenhouse for further analyses.

  20. PCR-based cDNA library construction: general cDNA libraries at the level of a few cells.

    OpenAIRE

    Belyavsky, A; Vinogradova, T; Rajewsky, K

    1989-01-01

    A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers complementary to oligo(dA) and oligo(dG) ends of the cDNA. For insertion of the cDNA into a vector a controlled trimming of the 3' ends of the cDNA by Klenow enzyme was used. Starting from 10 J558L ...

  1. Differential Gene Expression Reveals Candidate Genes for Drought Stress Response in Abies alba (Pinaceae.

    Directory of Open Access Journals (Sweden)

    David Behringer

    Full Text Available Increasing drought periods as a result of global climate change pose a threat to many tree species by possibly outpacing their adaptive capabilities. Revealing the genetic basis of drought stress response is therefore implemental for future conservation strategies and risk assessment. Access to informative genomic regions is however challenging, especially for conifers, partially due to their large genomes, which puts constraints on the feasibility of whole genome scans. Candidate genes offer a valuable tool to reduce the complexity of the analysis and the amount of sequencing work and costs. For this study we combined an improved drought stress phenotyping of needles via a novel terahertz water monitoring technique with Massive Analysis of cDNA Ends to identify candidate genes for drought stress response in European silver fir (Abies alba Mill.. A pooled cDNA library was constructed from the cotyledons of six drought stressed and six well-watered silver fir seedlings, respectively. Differential expression analyses of these libraries revealed 296 candidate genes for drought stress response in silver fir (247 up- and 49 down-regulated of which a subset was validated by RT-qPCR of the twelve individual cotyledons. A majority of these genes code for currently uncharacterized proteins and hint on new genomic resources to be explored in conifers. Furthermore, we could show that some traditional reference genes from model plant species (GAPDH and eIF4A2 are not suitable for differential analysis and we propose a new reference gene, TPC1, for drought stress expression profiling in needles of conifer seedlings.

  2. Differential Gene Expression Reveals Candidate Genes for Drought Stress Response in Abies alba (Pinaceae).

    Science.gov (United States)

    Behringer, David; Zimmermann, Heike; Ziegenhagen, Birgit; Liepelt, Sascha

    2015-01-01

    Increasing drought periods as a result of global climate change pose a threat to many tree species by possibly outpacing their adaptive capabilities. Revealing the genetic basis of drought stress response is therefore implemental for future conservation strategies and risk assessment. Access to informative genomic regions is however challenging, especially for conifers, partially due to their large genomes, which puts constraints on the feasibility of whole genome scans. Candidate genes offer a valuable tool to reduce the complexity of the analysis and the amount of sequencing work and costs. For this study we combined an improved drought stress phenotyping of needles via a novel terahertz water monitoring technique with Massive Analysis of cDNA Ends to identify candidate genes for drought stress response in European silver fir (Abies alba Mill.). A pooled cDNA library was constructed from the cotyledons of six drought stressed and six well-watered silver fir seedlings, respectively. Differential expression analyses of these libraries revealed 296 candidate genes for drought stress response in silver fir (247 up- and 49 down-regulated) of which a subset was validated by RT-qPCR of the twelve individual cotyledons. A majority of these genes code for currently uncharacterized proteins and hint on new genomic resources to be explored in conifers. Furthermore, we could show that some traditional reference genes from model plant species (GAPDH and eIF4A2) are not suitable for differential analysis and we propose a new reference gene, TPC1, for drought stress expression profiling in needles of conifer seedlings.

  3. Natural and synthetic oviposition stimulants forCatolaccus grandis (Burks) females.

    Science.gov (United States)

    Guerra, A A; Martinez, S; Sonia Del Rio, H

    1994-07-01

    Oviposition behavior was elicited fromCatolaccus grandis (Burks) (Hymenoptera: Pteromalidae) females, an ectoparasitoid of the boll weevil,Anthonomus grandis Boheman (Coleoptera: Curculionidae), by smears of freshly cut cotton bolls or smears of extracts prepared with boll weevil damaged or undamaged cotton boll tissues. Oviposition behavior was also elicited fromC. grandis females by smears made withn-pentane,n-hexane,n-heptane, and isooctane. This is the first report of oviposition behavior elicited for any parasitoid by these short-chain saturated hydrocarbons (alkanes), introducing a new concept on the chemical mediation of parasitoid behavior during host selection. Oviposition behavior was also elicited fromC. grandis females by volatiles emanating from an artificial diet devoid of insect components that was specifically developed for the in vitro rearing of ectoparasitoids. The possible use of a synergistic combination ofn-hexane and diet to optimize the mechanized production of noncontaminated eggs is also discussed.

  4. Contact Eczema Caused by True Teak (Tectona grandis)

    Science.gov (United States)

    Krogh, H. K.

    1964-01-01

    A follow-up study of a previous epidemiological investigation in a furniture factory concerning allergic contact eczema caused by true teak (Tectona grandis) clearly shows that a good educational campaign associated with specific and general prophylactic measures are able to control occupational dermatoses. At the first investigation 20·5% of the workers had allergic contact eczema and/or severe itching whereas only 8·3% suffered from the same complaints on re-examination. The different prophylactic measures previously recommended had been taken. Patch tests with various teak extracts were made on 13 workers sensitized to teak; these showed that lapachol (a derivative of naphthoquinone) is one of the sensitizing agents in teak wood. Furthermore, allergic reactions were obtained in seven of these workers when Jacaranda wood dust containing lapachol was used as the test substance. PMID:14106137

  5. Combining ability of elite clones of Eucalyptus grandis and Eucalyptus urophylla with Eucalyptus globulus

    OpenAIRE

    Bison,Odair; Ramalho,Magno Antonio Patto; Rezende,Gabriel Dehon Sampaio Peçanha; Aguiar,Aurélio Mendes; Resende,Marcos Deon Vilela de

    2007-01-01

    In Brazil, eucalyptus breeding programs for cellulose production has used two species, Eucalyptus grandis and Eucalyptus urophylla. Nevertheless, it would be useful to introgress alleles from other species to improve wood quality and volume. The objective of this research was to evaluate the hybrid potential of elite clones of E. grandis and E. urophylla from the Aracruz Celulose Company S. A. with Eucalyptus globulus clones. To do so, six elite clones were crossed with ten E. globulus clones...

  6. How wind affects growth in treeline Picea abies

    Czech Academy of Sciences Publication Activity Database

    Kašpar, J.; Hošek, Jiří; Treml, V.

    2017-01-01

    Roč. 127, č. 2 (2017), s. 1-12 ISSN 1664-2201 Institutional support: RVO:68378289 Keywords : height increment * picea abies * radial growth * thigmomorphogenesis * tree rings Subject RIV: GK - Forestry OBOR OECD: Forestry Impact factor: 2.281, year: 2016 https://link.springer.com/content/pdf/10.1007%2Fs00035-017-0186-x.pdf

  7. Notes Toward A Biography Of Ibn Abi Tahir Tayfur | Tourawa ...

    African Journals Online (AJOL)

    Notes Toward A Biography Of Ibn Abi Tahir Tayfur. SM Tourawa. Abstract. No Abstract. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL · News. OTHER RESOURCES.

  8. Resistance to Phytophthora cinnamomi in the Genus Abies

    Science.gov (United States)

    John Frampton; Fikret Isik; Mike Benson; Jaroslav Kobliha; Jan Stjskal

    2012-01-01

    A major limiting factor for the culture of true firs as Christmas trees is their susceptibility to Oomycete species belonging to the genus Phytophthora. In North Carolina alone, the Fraser fir (Abies fraseri [Pursh] Poir.) Christmas tree industry loses 6 to 7 million dollars annually to root rot primarily caused by ...

  9. Genetics and evolution of the Mediterranean Abies species

    Energy Technology Data Exchange (ETDEWEB)

    Parducci, Laura [Swedish Univ. of Agricultural Sciences, Umeaa (Sweden). Dept. of Forest Genetics and Plant Physiology

    2000-07-01

    This thesis summarizes and discusses results of five separate studies in which molecular techniques have been used to study the genetic variability and evolution of the Abies taxa occurring in the Mediterranean region. In particular, the investigation focused on the rare species Abies nebrodensis (Lojac.) Mattei, endemic to the island of Sicily, and the three neighbouring species A. alba (Mill.), A. cephalonica (Laud.) and A. numidica (De Lann.). The main aim of the studies was to determine the amount and distribution of the genetic variability within and among Mediterranean taxa of Abies, at both the nuclear and chloroplast levels, in order to elucidate their origin and evolution and to shed light on the taxonomic position of A. nebrodensis. In studies I, II and V allozyme markers were used to provide information on the level and distribution of genetic variation among and within natural populations of A. alba, A. cephalonica, A. nebrodensis and A. numidica and to estimate the outcrossing rate within A. alba. In studies III and IV, DNA markers from the chloroplast genome were developed and employed at the intra- and inter-specific levels to estimate the degree of cpDNA variation in the genus and to derive inferences concerning species relationships. Two different approaches were used: the first involved a comparative restriction-site analysis of ten different amplified chloroplast DNA fragments and the second involved the analysis of six chloroplast hypervariable repetitive simple-sequence repeats (cpSSRs or microsatellites). Analyses of both allozyme and cpDNA data indicated that A. nebrodensis differs from the neighbouring Abies species and justified its classification as a separate species. Based on results from studies III, IV and V we suggested that A. nebrodensis originated during the Messinian crises of the latest part of the Miocene, through hybridisation between A. alba and A. numidica. During the warmer period of the Holocene A. nebrodensis became more

  10. Lectin cDNA and transgenic plants derived therefrom

    Science.gov (United States)

    Raikhel, Natasha V.

    2000-10-03

    Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

  11. Foliar morphological and physiological plasticity in Picea abies and Abies alba saplings along a natural light gradient.

    Science.gov (United States)

    Grassi, G; Bagnaresi, U

    2001-08-01

    The role of morphological versus physiological foliar plasticity in the capacity for, and mechanisms of, photosynthetic acclimation was assessed in Picea abies (L.) Karst. and Abies alba Mill. saplings in a forest gap-understory light gradient (relative irradiance, RI, ranging from 0.02 to 0.32). The species investigated showed a similar foliar morphological plasticity along the light gradient, at both the needle level (through alteration in leaf dry mass per area) and the shoot level (through alteration in the silhouette area ratio, e.g., shoot silhouette to projected needle area ratio). In both species chlorophyll (Chl) concentration on a mass basis decreased at increasing RI, but was independent of RI when expressed on an area basis. In contrast, leaf N concentration on a mass basis was independent of RI, but was positively influenced by RI when expressed on an area basis. The parameters describing photosynthetic performance at low light (dark respiration rate, apparent quantum yield and light compensation point) suggest that Abies alba was better suited to maintain a positive carbon balance in shaded conditions. By contrast, parameters describing biochemical capacity at high light (maximum electron transport rate, Jmax and maximum ribulose-1,5-biphosphate carboxylation capacity, Vcmax) indicate that only Picea abies was capable of acclimating physiologically to high photosynthetic photon flux densities (PPFDs) by increasing nitrogen partitioning to Rubisco and Vcmax/mass by increasing RI. These results support the hypothesis that interspecific differences in nitrogen partitioning within the photosynthetic apparatus may provide a mechanistic basis for species separation along a light gradient. The differences in photosynthetic plasticity observed are likely to influence regeneration patterns and habitat breadth of the species investigated. The limited ability of Abies alba saplings to exploit high-light conditions may be a competitive disadvantage in large

  12. Cloning, sequencing and expression of cDNA encoding growth ...

    Indian Academy of Sciences (India)

    Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3′ region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length ...

  13. The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein.

    Science.gov (United States)

    Zhao, Wenming; Guan, Chunmei; Feng, Jian; Liang, Yan; Zhan, Ni; Zuo, Jianru; Ren, Bo

    2016-07-01

    In Arabidopsis, the phytohormone abscisic acid (ABA) plays a vital role in inhibiting seed germination and in post-germination seedling establishment. In the ABA signaling pathway, ABI5, a basic Leu zipper transcription factor, has important functions in the regulation of seed germination. ABI5 protein localizes in nuclear bodies, along with AFP, COP1, and SIZ1, and was degraded through the 26S proteasome pathway. However, the mechanisms of ABI5 nuclear body formation and ABI5 protein degradation remain obscure. In this study, we found that the Arabidopsis CROWDED NUCLEI (CRWN) proteins, predicted nuclear matrix proteins essential for maintenance of nuclear morphology, also participate in ABA-controlled seed germination by regulating the degradation of ABI5 protein. During seed germination, the crwn mutants are hypersensitive to ABA and have higher levels of ABI5 protein compared to wild type. Genetic analysis suggested that CRWNs act upstream of ABI5. The observation that CRWN3 colocalizes with ABI5 in nuclear bodies indicates that CRWNs might participate in ABI5 protein degradation in nuclear bodies. Moreover, we revealed that the extreme C-terminal of CRWN3 protein is necessary for its function in the response to ABA in germination. Our results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination. © 2015 Institute of Botany, Chinese Academy of Sciences.

  14. cDNA sequence quality data - Budding yeast cDNA sequencing project | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us Budding yeast cDNA sequencing project cDNA sequence quality data Data detail Data name cDNA sequence quality... data DOI 10.18908/lsdba.nbdc00838-003 Description of data contents Phred's quality score. P...tion Download License Update History of This Database Site Policy | Contact Us cDNA sequence quality

  15. Estimativa do tempo de vaporização de toras de Eucalyptus grandis Steaming times estimates for Eucalyptus grandis logs

    Directory of Open Access Journals (Sweden)

    Fred Willians Calonego

    2006-06-01

    Full Text Available O objetivo deste estudo foi adequar o modelo geral de determinação do tempo de vaporização de toras, proposto por Steinhagen et al. (1980, para a madeira de Eucalyptus grandis. Para tanto, foram coletadas toras de 20 a The aim this study was to adjust the general model for determining log steaming time, proposed by Steinhagen et al. (1980, for Eucalyptus grandis wood. In order to do so, logs with diameter from 20 to <25, 25 to <30 and 30 to <35 cm were collected from 14 trees of Eucalyptus grandis derived from the `Horto Florestal' nursery, Manduri, São Paulo. A thermocouple was inserted into each log near its center. The logs were steamed during 20 hours at 90ºC and 100% relative humidity. A data logger recorded the temperatures during the thermal treatment. It was concluded that the Steinhagen et al. (1980 model cannot be directly used for this species in study and corrections factors are proposed for the utilization of the general model for log steaming time, developed by Steinhagen et al. (1980, for Eucalyptus grandis wood.

  16. E de Eucalyptus grandis W. Hill ex Maiden

    Directory of Open Access Journals (Sweden)

    Mara Rubia Silva

    2005-01-01

    Full Text Available This work had the objective of study in patterns of the “speckle” as function of the wood texture of Bowdichia virgilioides (sucupira, Swietenia macrophylla (mogno, Balfourodendron riedelianum (paumarfim and Eucalyptus grandis wood. The He-Ne laser 10 mW was used to illuminate the wood samples and a digital camera captured the result of the interaction of the wood with the laser light. The results obtained from image analysis were compared with the pattern obtained by microscopic methodology. The captured images show the intensities of each pixel, varying between 0 and 255 in the gray scale. From the images, a central area of 16x16 pixels was removed and analyzed by the application of the convolution function and its Fourier Transform, resulting in the power density spectrum. The results showed that the information obtained by the power density spectrum of the “speckle” space variation allow classing samples of woods with different texture.

  17. Th, Pa and U isotopes in an echinoderm, Encope grandis

    International Nuclear Information System (INIS)

    Omura, Akio; Ku, Teh-Lung.

    1979-01-01

    The application of 230 Th and 231 Pa growth methods to the hard tissues of living things, which are effective for the radiometric age measurement for latter Quaternary period, has been limited to certain corals, therefore it has been scarcely utilized in other areas than coral reefs. Reef coral fossils (Porites) were obtained from terrace deposits of Magdalena Island in Southern Baja California, and the methods were applied to them. At the time, the isotope compositions of Th, Pa and U in the shells of echinoderm Encope Grandis and of the living samples were examined. The estimated ages were in agreement with those of coral. It suggests that the reliable 230 Th and 231 Pa ages of sea-urchin fossils were presented for the first time and that the method is applicable to such fossils only if the conditions can be met. The results are highly significant, since the method may be used in other areas than coral reefs. (J.P.N.)

  18. Efficient and new method for Tectona grandis in vitro regeneration

    Directory of Open Access Journals (Sweden)

    Evandro V. Tambarussi

    2017-04-01

    Full Text Available The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1 and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1 resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.

  19. ECONOMIC EVALUATION OF Eucalyptus grandis PLANTATION FOR CELLULOSE PRODUCTION

    Directory of Open Access Journals (Sweden)

    Antonio Donizette de Oliveira

    2008-03-01

    Full Text Available The aims of this research were: to analyze the economic feasibility of planting eucalyptus for producing wood pulp,considering various site index and two spacings; to analyze the economic effects regarding the profitability of the forest activity indifferent distances from the industry and changes on discount rate, wood price, transportation costs, minimum profitable diameter oflogs and the length of the logs. A biometric model for making wood volume prognosis was developed, using data of a trial ofEucalyptus grandis stands 19 and 103 months old. The prognosis started at the age zero, considering logs of 2.5 and 6.0 m of lenghtand the minimum diameter varying from 4 to 10 cm, in intervals of 2 cm. Net Present Worth (NPW was used as the economic decisioncriterium, considering an infinite horizon. The main conclusions were: reducing the minimum profitable diameter and the length ofthe logs are good strategies to increase wood utilization and profit; plantations located in less productive lands are economicallyunfeasible; the cost of transportation has significant effect on the profitability of the forest activity and must be analyzed carefully atthe moment of defining the location of new plantations; small variations on wood sales price may cause big alterations on theprofitability of the forest activity, suggesting that the improvement of the wood quality together with other decisions that may increasewood price are alternatives that may render the plantations in less productive areas profitable.

  20. Sewage sludge as substrate for Tectona grandis L. seedlings production

    Directory of Open Access Journals (Sweden)

    Daniele Rodrigues Gomes

    2013-03-01

    Full Text Available The sewage sludge is a waste from sewage treatment plants, which can be used in formulations of substrates for seedling production. This study aimed to evaluate the effects of different proportions of sewage sludge, soil and commercial substrate on the growth characteristics of Tectona grandis seedlings in tubes of 120 cm³ volume capacity. The sewage sludge used came from the STP's Cachoeiro de Itapemirim / ES. The seedlings were grown in the forest nursery / CCA / UFES. The statistical design used in the experiment was completely randomized design (CRD with six treatments and five replications. The treatments constituted of sewage sludge : commercial substrate: soil (v: v: v, which corresponded to 20:70:10 (T1, 40:50:10 (T2, 60:30:10 (T3, 80 : 10:10 (T4, 90:0:10 (T5 and the control treatment with 0:90:10 (T6. All variables assessed were significant at the 5% level of probability. The control (T6 showed results statistically equal to or lower than the treatments T3 with 60% of sewage sludge concentration (T3. It follows then that the use of sewage sludge in seedling production is feasible and promising, however, used in a proper proportion.

  1. ABI3 mediates dehydration stress recovery response in Arabidopsis thaliana by regulating expression of downstream genes.

    Science.gov (United States)

    Bedi, Sonia; Sengupta, Sourabh; Ray, Anagh; Nag Chaudhuri, Ronita

    2016-09-01

    ABI3, originally discovered as a seed-specific transcription factor is now implicated to act beyond seed physiology, especially during abiotic stress. In non-seed plants, ABI3 is known to act in desiccation stress signaling. Here we show that ABI3 plays a role in dehydration stress response in Arabidopsis. ABI3 gene was upregulated during dehydration stress and its expression was maintained during subsequent stress recovery phases. Comparative gene expression studies in response to dehydration stress and stress recovery were done with genes which had potential ABI3 binding sites in their upstream regulatory regions. Such studies showed that several genes including known seed-specific factors like CRUCIFERIN1, CRUCIFERIN3 and LEA-group of genes like LEA76, LEA6, DEHYDRIN LEA and LEA-LIKE got upregulated in an ABI3-dependent manner, especially during the stress recovery phase. ABI3 got recruited to regions upstream to the transcription start site of these genes during dehydration stress response through direct or indirect DNA binding. Interestingly, ABI3 also binds to its own promoter region during such stress signaling. Nucleosomes covering potential ABI3 binding sites in the upstream sequences of the above-mentioned genes alter positions, and show increased H3 K9 acetylation during stress-induced transcription. ABI3 thus mediates dehydration stress signaling in Arabidopsis through regulation of a group of genes that play a role primarily during stress recovery phase. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  2. Validation of early GOES-16 ABI on-orbit geometrical calibration accuracy using SNO method

    Science.gov (United States)

    Yu, Fangfang; Shao, Xi; Wu, Xiangqian; Kondratovich, Vladimir; Li, Zhengping

    2017-09-01

    The Advanced Baseline Imager (ABI) onboard the GOES-16 satellite, which was launched on 19 November 2016, is the first next-generation geostationary weather instrument in the west hemisphere. It has 16 spectral solar reflective and emissive bands located in three focal plane modules (FPM): one visible and near infrared (VNIR) FPM, one midwave infrared (MWIR), and one longwave infrared (LWIR) FPM. All the ABI bands are geometeorically calibrated with new techniques of Kalman filtering and Global Positioning System (GPS) to determine the accurate spacecraft attitude and orbit configuration to meet the challenging image navigation and registration (INR) requirements of ABI data. This study is to validate the ABI navigation and band-to-band registration (BBR) accuracies using the spectrally matched pixels of the Suomi National Polar-orbiting Partnership (SNPP) Visible Infrared Imaging Radiometer Suite (VIIRS) M-band data and the ABI images from the Simultaneous Nadir Observation (SNO) images. The preliminary results showed that during the ABI post-launch product test (PLPT) period, the ABI BBR errors at the y-direction (along the VIIRS track direction) is smaller than at the x-direction (along the VIIRS scan direction). Variations in the ABI BBR calibration residuals and navigation difference to VIIRS can be observed. Note that ABI is not operational yet and the data is experimental and still under testing. Effort is still ongoing to improve the ABI data quality.

  3. Effect of thermal modification on the physical properties of juvenile and mature woods of Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Fred Willians Calonego

    Full Text Available This study aimed to evaluate the effect of thermal treatment on the physical properties of juvenile and mature woods of Eucalyptus grandis. Boards were taken from 30-year-old E. grandis trees. The boards were thermally modified at 180 °C in the Laboratory of Wood Drying and Preservation at UNESP, Botucatu, Sao Paulo state, Brazil. The results showed that thermal modification caused: (1 decrease of 6.8% in the density at 0% equilibrium moisture content of mature wood; (2 significant decreases of 14.7% and 35.6% in the maximum volumetric swellings of juvenile and mature woods, respectively; (3 significant decreases of 13.7% and 21.3% in the equilibrium moisture content of juvenile and mature woods, respectively. The influence of thermal modification in juvenile wood was lower than in mature wood and caused greater uniformity in the physical variations between these types of wood in E. grandis.

  4. Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina In vitro multiplication of Eucalyptus grandis under BAP pulse

    Directory of Open Access Journals (Sweden)

    Wirifran Fernandes de Andrade

    2006-12-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos do estímulo com benzilaminopurina (BAP na multiplicação in vitro de Eucalyptus grandis. Foram avaliadas as interações entre concentrações de BAP (0, 200, 400 e 600 mg L-1, tempo de exposição (1, 2 e 3 horas, pH (3 e 5,8 da solução e alterações morfológicas dos explantes. Semanalmente, foi determinada a massa da matéria fresca dos explantes. Aos 21 dias de cultura, foram avaliados: o número de brotações por tratamento, o número de brotações obtidas por explante (taxa de multiplicação, e foi realizado o resgate das plântulas para avaliação histológica. O pH não apresentou interação com os demais fatores estudados. Os tratamentos com BAP a 200 mg L-1, durante 1 e 2 horas, apresentaram-se como os mais indicados na multiplicação do E. grandis. Houve intensificação da divisão celular, no parênquima cortical e no procâmbio, representada pelo surgimento de meristemóides, em resposta aos tratamentos com 200 mg L-1 de BAP, durante 1 e 2 horas. O estímulo com BAP na multiplicação in vitro de E. grandis é viável.The objective of this work was to evaluate the effect of pulse on in vitro multiplication of Eucalyptus grandis. Interactions were evaluated among BAP concentrations (0, 200, 400 and 600 mg L-1, exposure time (1, 2 and 3 hours, pH values (3 and 5.8, and explant morphological changes. Fresh weight of the explants was determined weekly. At 21 days of culture, valuations were made of the number of shootings per treatment, number of shootings obtained per explant (multiplication rate, and seedlings rescue was accomplished for histological analysis. The pH values did not present any interaction with the other factors. The most significant treatments on E. grandis shoot multiplications were 200 mg L-1 of 6-BAP during 1 and 2 hours. There was an intensification of cell division in the cortical parenchyma and procambium, represented by the arising of meristems in

  5. Cooperation of three WRKY-domain transcription factors WRKY18, WRKY40, and WRKY60 in repressing two ABA-responsive genes ABI4 and ABI5 in Arabidopsis.

    Science.gov (United States)

    Liu, Zhi-Qiang; Yan, Lu; Wu, Zhen; Mei, Chao; Lu, Kai; Yu, Yong-Tao; Liang, Shan; Zhang, Xiao-Feng; Wang, Xiao-Fang; Zhang, Da-Peng

    2012-11-01

    Three evolutionarily closely related WRKY-domain transcription factors WRKY18, WRKY40, and WRKY60 in Arabidopsis were previously identified as negative abscisic acid (ABA) signalling regulators, of which WRKY40 regulates ABI4 and ABI5 expression, but it remains unclear whether and how the three transcription factors cooperate to regulate expression of ABI4 and ABI5. In the present experiments, it was shown that WRKY18 and WRKY60, like WRKY40, interact with the W-box in the promoters of ABI4 and ABI5 genes, though the three WRKYs have their own preferential binding domains in the two promoters. WRKY18 and WRKY60, together with WRKY40, inhibit expression of the ABI5 and/or ABI4 genes, which is consistent with their negative roles in ABA signalling. Further, genetic evidence is provided that mutations of ABI4 and ABI5 genes suppress ABA-hypersensitive phenotypes of the null mutant alleles of WRKY18 and WRKY60 genes, demonstrating that ABI4 and ABI5 function downstream of these two WRKY transcription factors in ABA signalling. A working model of cooperation of the three WRKYs in repressing ABI4 and ABI5 expression is proposed, in which the three WRKYs antagonize or aid each other in a highly complex manner. These findings help to understand the complex mechanisms of WRKY-mediated ABA signal transduction.

  6. Modification of static bending strength properties of Eucalyptus grandis heat-treated wood

    OpenAIRE

    Cademartori,Pedro Henrique Gonzalez de; Schneid,Eduardo; Gatto,Darci Alberto; Beltrame,Rafael; Stangerlin,Diego Martins

    2012-01-01

    The present study describes the effect of thermal rectification on physical and mechanical properties of Eucalyptus grandis wood at different levels of temperature and time. Samples of Eucalyptus grandis wood (10 × 10 × 200 mm) were heat-treated at 180, 200, 220 and 240 °C during 4 and 8 hours. The mechanical properties of heat-treated and untreated samples were determined by static bending tests. The physical properties were determined by weight loss and swelling tests. The results showed th...

  7. Anthraquinones and naphtoquinones from the bark of Tectona grandis (Verbenaceae) reforestation specimen

    OpenAIRE

    Moreira, Rafael Y.O.; Arruda, Mara S.P.; Arruda, Alberto C.; Santos, Lourivaldo S.; Müller, Adolfo H.; Guilhon, Giselle M.S.P.; Santos, Alberdan S.; Terezo, Evaristo

    2006-01-01

    O fracionamento do extrato hexânico do caule de um espécime de reflorestamento de Tectona grandis (Verbenaceae), através de procedimentos fitoquímicos clássicos, levou ao isolamento das naftoquinonas lapachol e desidro-a-lapachona e das antraquinonas tectoquinona e obtusifolina. As estruturas das substâncias foram caracterizadas através da análise de métodos espectrométricos de RMN. Este é o primeiro estudo fitoquímico de um espécime de reflorestamento de Tectona grandis, no Brasil, sendo o o...

  8. Effect of antibiotic ceftriaxone on elimination of ABI and GV3101 strains of Agrobacterium tumefaciens

    Directory of Open Access Journals (Sweden)

    Gorbatyuk I. R.

    2015-12-01

    Full Text Available Aim. To find out, at which concentration the antibiotic ceftriaxone of β-lactam group causes the elimination of ABI and GV3101 strains of Agrobacterium tumefaciens. Methods. The disc diffusion method. Results. Antibiotic ceftriaxone was used for the cell elimination of the Agrobacterium tumefaciens ABI strain for the first time. The same zones of inhibition were observed when using the 400 mg/l ceftriaxone and 500 mg/l cefotaxime solutions for both Agrobacterium strains (ABI and GV3101 studied. Conclusions. Ceftriaxone inhibits the Agrobacterium growth more effectively than cefotaxime. The ceftriaxone concentration for elimination of ABI and GV3101 Agrobacterium tumefaciens strains is 400 mg/l.

  9. Activation and Transfer of the Chromosomal Phage Resistance Mechanism AbiV in Lactococcus lactis

    DEFF Research Database (Denmark)

    Haaber, J.; Moineau, S.; Hammer, Karin

    2009-01-01

    AbiV is a chromosomally encoded phage resistance mechanism that is silent in the wild-type phage-sensitive strain Lactococcus lactis subsp. cremoris MG1363. Spontaneous phage-resistant mutants of L. lactis MG1363 were analyzed by reverse transcriptase PCR and shown to express AbiV. This expression...... was related to a reorganization in the upstream region of abiV. Transfer of abiV between two lactococcal strains, most likely by conjugation, was also demonstrated. To our knowledge, this is the first report of natural transfer of a chromosomally encoded phage resistance mechanism....

  10. Henri Frankfort, Aby Warburg and “Mythopoeic Thought”

    Directory of Open Access Journals (Sweden)

    Paul Taylor

    2011-12-01

    Full Text Available This is the text of a lunchtime lecture given at the Warburg Institute in the spring of 2004. It formed part of a series concerned with past Warburg scholars, and was devoted to the work of Henri Frankfort, Director of the Institute from 1949 to 1954. The first third of the lecture was a rapid account of his career; the remainder of the lecture was an analysis and criticism of the concept of ‘primitive thinking’ in the work of Frankfort and Aby Warburg.

  11. Dynamic base-age invariant site index models for Tectona grandis ...

    African Journals Online (AJOL)

    Data from 27 remeasured sample plots were used to evaluate dynamic base-age invariant site index models for teak (Tectona grandis) forests in Karnataka, India. The data were obtained in observational field studies covering a wide range of sites in Karnataka and provided up to three interval measurements per plot.

  12. Antibacterial, antioxidant and cell proliferative properties ofCoccinia grandis fruits.

    Science.gov (United States)

    Sakharkar, Prashant; Chauhan, Balwantsinh

    2017-01-01

    Little knowledge is available on the antimicrobial and antioxidant properties of Coccina grandis fruits and no study has reported on its cell proliferative property. The aim of this study was to examine the antimicrobial, antioxidant and cell proliferative property of fruits of C. grandis . Fruits of C . grandis were extracted using water; ethanol and acetone by cold and hot Soxhlet extraction. The antibacterial activities of the extracts were tested against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa using the modified Kirby-Bauer diffusion method and compared against erythromycin. The antioxidant property was determined using Cayman's antioxidant assay; whereas cell proliferation/cytotoxic properties were evaluated using the Cell Titer 96 Aqueous One Solution Cell MTS assay with MDA-MB 321 breast cancer cells. Data were analyzed for correlation and differences using unpaired student's t-test and one-way ANOVA. A p value of fruits showed some degree of bacterial growth inhibition. Acetone extracts exhibited higher antibacterial activity. Both ethanol extracts showed antioxidant property when compared with standard Trolox. In contrary to cytotoxicity, all four extracts showed cell proliferation compared to controls at different concentrations. However, acetone extracts exhibited greater cell proliferation compared to ethanol extracts and cold extracts performed better than the hot extracts. C. grandis fruits exhibited some degree of antimicrobial, antioxidant and cell proliferative properties. Further investigation is warranted to isolate, confirm and characterize phytochemicals that are responsible for the medicinal properties observed.

  13. Intercropping Acacia mangium stimulates AMF colonization and soil phosphatase activity in Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Daniel Bini

    Full Text Available ABSTRACT: Arbuscular mycorrhizal fungi (AMF are very important to plant nutrition, mostly in terms of acquisition of P and micronutrients. While Acacia mangium is closely associated with AMF throughout the whole cycle, Eucalyptus grandis presents this symbiosis primarily at the seedling stage. The aim of this study was to evaluate the dynamics of AMF in these two tree species in both pure and mixed plantations during the first 20 months after planting. We evaluated the abundance, richness and diversity of AMF spores, the rate of AMF mycorrhizal root colonization, enzymatic activity and soil and litter C, N and P. There was an increase in AMF root colonization of E. grandis when intercropped with A. mangium as well as an increase in the activity of acid and alkaline phosphatase in the presence of leguminous trees. AMF colonization and phosphatase activities were both involved in improvements in P cycling and P nutrition in soil. In addition, P cycling was favored in the intercropped plantation, which showed negative correlation with litter C/N and C/P ratios and positive correlation with soil acid phosphatase activity and soil N and P concentrations. Intercropping A. mangium and E. grandis maximized AMF root colonization of E. grandis and phosphatase activity in the soil, both of which accelerate P cycling and forest performance.

  14. Above-ground dry matter accumulation by Eucalyptus grandis and its relation to standard meteorological data

    International Nuclear Information System (INIS)

    Byrne, G.F.

    1989-01-01

    The relationship between the rate of increase of biomass in some stands of Eucalyptus grandis, growing near Coffs Harbour, N.S.W., Australia, is explored in terms of estimated evapotranspiration and radiation interception, and related to a similar previous study of Pinus radiata. A possible role of method of planting, site slope and site aspect in biomass increase is also discussed

  15. Ozone bleaching of South African Eucalyptus grandis kraft pulps containing high levels of hexenuronic acids

    CSIR Research Space (South Africa)

    Andrew, JE

    2013-08-01

    Full Text Available was a preliminary investigation into the use of ozone in bleaching sequences for kraft pulps produced from South African Eucalyptus grandis wood chips, which typically contained high amounts of hexenuronic acids (HexA). The objective of the study...

  16. Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using ...

    African Journals Online (AJOL)

    Given the current demand for timber from forest species, there is a need to develop new strategies for the mass propagation of eucalyptus. Therefore, this study aimed to determine the effect of different doses of Standak Top® and CoMo Raiz® on the establishment of Eucalyptus grandis W. Hill ex Maiden from seeds in vitro.

  17. Modelo empirico integral de una plantacion de Eucalyptus grandis en Concordia, Entre Rios

    Science.gov (United States)

    Jorge Frangi; Carolina Perez; Juan Goya; Natalia Teson; Marcelo Barrera; Marcelo Arturi

    2016-01-01

    The Argentinian Mesopotamia is the core of fast-growing tree species plantations of the country. Eucalyptus grandis plantations constitute 90 % of the forested area with Eucalyptus spp. in NE Entre Rios. Based on previous studies on structural and functional features, a comprehensive model is here proposed on emergence of new properties linked to matter and ecosystem...

  18. In vitro antioxidant activities of the fractions of Coccinia grandis l. leaf ...

    African Journals Online (AJOL)

    The present study was aimed at investigating the antioxidant activities of the various fractions of the hydromethanolic extract of the leaves of Coccinia grandis L. Voigt. (Cucurbitaceae). The antioxidant activities of the fractions have been evaluated by using nine in vitro assays and were compared to standard antioxidants ...

  19. Tectona grandis L. f (the teak tree; Hindi: Sagallll or Segllll) (~r ...

    Indian Academy of Sciences (India)

    Tectona grandis L. f (the teak tree; Hindi: Sagallll or Segllll) (~r Verhell({Ceae is a large deciduous tree cllitivatedfor its timber. Leaves are large; flmvers are small, white, svveet-scented and borne on highly branched inflorescences. Fruit is hard and enveloped by bladder-like cal.vx. The timber is one of the best for all wood ...

  20. High-resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly.

    Science.gov (United States)

    Bartholomé, Jérôme; Mandrou, Eric; Mabiala, André; Jenkins, Jerry; Nabihoudine, Ibouniyamine; Klopp, Christophe; Schmutz, Jeremy; Plomion, Christophe; Gion, Jean-Marc

    2015-06-01

    Genetic maps are key tools in genetic research as they constitute the framework for many applications, such as quantitative trait locus analysis, and support the assembly of genome sequences. The resequencing of the two parents of a cross between Eucalyptus urophylla and Eucalyptus grandis was used to design a single nucleotide polymorphism (SNP) array of 6000 markers evenly distributed along the E. grandis genome. The genotyping of 1025 offspring enabled the construction of two high-resolution genetic maps containing 1832 and 1773 markers with an average marker interval of 0.45 and 0.5 cM for E. grandis and E. urophylla, respectively. The comparison between genetic maps and the reference genome highlighted 85% of collinear regions. A total of 43 noncollinear regions and 13 nonsynthetic regions were detected and corrected in the new genome assembly. This improved version contains 4943 scaffolds totalling 691.3 Mb of which 88.6% were captured by the 11 chromosomes. The mapping data were also used to investigate the effect of population size and number of markers on linkage mapping accuracy. This study provides the most reliable linkage maps for Eucalyptus and version 2.0 of the E. grandis genome. © 2014 CIRAD. New Phytologist © 2014 New Phytologist Trust.

  1. ABI5 Is a Regulator of Seed Maturation and Longevity in Legumes.

    Science.gov (United States)

    Zinsmeister, Julia; Lalanne, David; Terrasson, Emmanuel; Chatelain, Emilie; Vandecasteele, Céline; Vu, Benoit Ly; Dubois-Laurent, Cécile; Geoffriau, Emmanuel; Signor, Christine Le; Dalmais, Marion; Gutbrod, Katharina; Dörmann, Peter; Gallardo, Karine; Bendahmane, Abdelhafid; Buitink, Julia; Leprince, Olivier

    2016-11-01

    The preservation of our genetic resources and production of high-quality seeds depends on their ability to remain viable and vigorous during storage. In a quantitative trait locus analysis on seed longevity in Medicago truncatula, we identified the bZIP transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5). Characterization of Mt-abi5 insertion mutant seeds revealed that both the acquisition of longevity and dormancy were severely impaired. Using transcriptomes of developing Mt-abi5 seeds, we created a gene coexpression network and revealed ABI5 as a regulator of gene modules with functions related to raffinose family oligosaccharide (RFO) metabolism, late embryogenesis abundant (LEA) proteins, and photosynthesis-associated nuclear genes (PhANGs). Lower RFO contents in Mt-abi5 seeds were linked to the regulation of SEED IMBIBITION PROTEIN1 Proteomic analysis confirmed that a set of LEA polypeptides was reduced in mature Mt-abi5 seeds, whereas the absence of repression of PhANG in mature Mt-abi5 seeds was accompanied by chlorophyll and carotenoid retention. This resulted in a stress response in Mt-abi5 seeds, evident from an increase in α-tocopherol and upregulation of genes related to programmed cell death and protein folding. Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes. © 2016 American Society of Plant Biologists. All rights reserved.

  2. Ankle-brachial index (ABI), abdominal aortic calcification (AAC), and coronary artery calcification (CAC): the Jackson heart study.

    Science.gov (United States)

    Tullos, Bobby W; Sung, Jung Hye; Lee, Jae Eun; Criqui, Michael H; Mitchell, Marc E; Taylor, Herman A

    2013-04-01

    To examine the associations of peripheral atherosclerosis, assessed by the ABI at baseline with the extent of AAC and with CAC measured by MDCT at follow-up examination in the Jackson Heart Study cohort. Four categories of ABI: 1.40. Presence of CAC/AAC was defined as scoring above the 75th percentile among participants with non-zero CT calcium scores. We conducted multivariable log-binomial models for this analysis examining the relationship between ABI and the presence of CAC or AAC using normal ABI (1.0 ≤ ABI ≤ 1.39) as the reference group. We estimated prevalence ratios adjusted for age, smoking, HTN, DM, BMI, LDL, HDL, CRP, systolic and diastolic blood pressure, and use of lipid-lowering medication. There were 2,398 patients in this analysis (women: 65 %, average age 55 years). AAC scores were not significantly different between sex. CAC scores were significantly higher in males than females regardless of ABI groups. The prevalence of significant AAC was 1.7 times higher for ABI AAC prevalence did not differ between subjects with ABI > 1.40 compared to those with normal ABI. The prevalence of the significant CAC was higher for ABI 1.40 compared to those with normal ABI. Lower ABI was significantly associated with the extent of AAC and CAC in this cohort. ABI can provide clinicians with an inexpensive additional tool to assess vascular health and cardiovascular risk without exposing the patient to ionizing radiation.

  3. Governo del territorio e grandi trasformazioni nella regione urbana milanese

    Directory of Open Access Journals (Sweden)

    Fulvia Pinto

    2010-02-01

    Full Text Available  Lo studio analizza le grandi trasformazioni in atto nella “regione urbana” milanese, esaminando gli strumenti, le strategie e le politiche seguite. Per “regione urbana” si intende un territorio che non fa riferimento a relazioni gerarchiche di tipo centro-periferico, bensì determina relazioni multi direzionali, a differenza dell’area metropolitana che viene definita in base a relazioni di dipendenza (economica, sociale, funzionale, ecc. di agglomerati esterni rispetto ad un capoluogo. La regione urbana milanese è uno dei poli economici e produttivi più attivi a livello nazionale ed europeo, tuttavia tale territorio è caratterizzato da una ridotta qualità urbana e ambientale e da fenomeni di polarizzazione e di frammentazione sociale in aumento, che hanno ripercussioni negative sulle prospettive di sviluppo dell'area. I più recenti strumenti di pianificazione dell’area milanese fanno riferimento alla coesione territoriale, cercando di disegnare una nuova struttura per il territorio frammentato e diffuso, consolidando il policentrismo della regione urbana, favorendo il riconoscimento di aggregazioni intermedie, al cui interno ricercare integrazione del sistema dei servizi, identità e appartenenze di area. Per attivare una politica di riqualificazione a livello urbano e territoriale, risulta fondamentale seguire forme di coordinamento tra i vari livelli di pianificazione. Particolare attenzione viene posta al problema della mobilità che richiede strategie particolari non solo del traffico quale effetto indotto, ma anche delle cause che lo producono, con riferimento specifico alla distribuzione sul territorio delle diverse attività, ad un corretto equilibrio tra insediamenti residenziali, produttivi e terziari. È necessario, dunque, individuare strumenti e procedure, capaci di perseguire un equilibrio dinamico tra i flussi attratti dalle attività insediate e i flussi “sostenibili” dalle infrastrutture di trasporto

  4. Aby Warburg, Images and Exhibitions. Aby Warburg, Bilderreihen und Ausstellungen edited by Uwe Fleckner and Isabelle Woldt, Akademie Verlag, 2012

    Directory of Open Access Journals (Sweden)

    Matthew Rampley

    2012-12-01

    Full Text Available This article reviews the latest volume in the collected works of Aby Warburg published by Akademie Verlag. The volume consists of exhibitions and plates of images Warburg compiled to illustrate lectures in the period between 1925-1929. The review focuses on two key issues raised by the publication: the light it casts on the Mnemosyne Atlas Warburg was working on at the same time, and, in particular, how it helps shape perceptions of the broader intellectual direction of Warburg's thinking in the final half decade of his life.

  5. The complete chloroplast genome sequence of Abies nephrolepis (Pinaceae: Abietoideae

    Directory of Open Access Journals (Sweden)

    Dong-Keun Yi

    2016-06-01

    Full Text Available The plant chloroplast (cp genome has maintained a relatively conserved structure and gene content throughout evolution. Cp genome sequences have been used widely for resolving evolutionary and phylogenetic issues at various taxonomic levels of plants. Here, we report the complete cp genome of Abies nephrolepis. The A. nephrolepis cp genome is 121,336 base pairs (bp in length including a pair of short inverted repeat regions (IRa and IRb of 139 bp each separated by a small single copy (SSC region of 54,323 bp (SSC and a large single copy region of 66,735 bp (LSC. It contains 114 genes, 68 of which are protein coding genes, 35 tRNA and four rRNA genes, six open reading frames, and one pseudogene. Seventeen repeat units and 64 simple sequence repeats (SSR have been detected in A. nephrolepis cp genome. Large IR sequences locate in 42-kb inversion points (1186 bp. The A. nephrolepis cp genome is identical to Abies koreana’s which is closely related to taxa. Pairwise comparison between two cp genomes revealed 140 polymorphic sites in each. Complete cp genome sequence of A. nephrolepis has a significant potential to provide information on the evolutionary pattern of Abietoideae and valuable data for development of DNA markers for easy identification and classification.

  6. cDNA structure, genomic organization and expression patterns of ...

    African Journals Online (AJOL)

    Visfatin was a newly identified adipocytokine, which was involved in various physiologic and pathologic processes of organisms. The cDNA structure, genomic organization and expression patterns of silver Prussian carp visfatin were described in this report. The silver Prussian carp visfatin cDNA cloned from the liver was ...

  7. cDNA structure, genomic organization and expression patterns of ...

    African Journals Online (AJOL)

    use

    2011-11-23

    Nov 23, 2011 ... Visfatin was a newly identified adipocytokine, which was involved in various physiologic and pathologic processes of organisms. The cDNA structure, genomic organization and expression patterns of silver Prussian carp visfatin were described in this report. The silver Prussian carp visfatin. cDNA cloned ...

  8. Thinking Allowed: Use of Egocentric Speech after Acquired Brain Injury (ABI)

    Science.gov (United States)

    Rees, Sian A.; Skidmore, David

    2011-01-01

    This paper explores the use of thinking aloud made by young people who have sustained a severe acquired brain injury (ABI). The phenomenon is compared with the concepts of egocentric speech and inner speech before the form of thinking aloud by pupils with ABI is examined. It is suggested that by using thinking aloud, this group of pupils is able…

  9. Prevalence and association of oral candidiasis with dysphagia in individuals with acquired brain injury (ABI)

    DEFF Research Database (Denmark)

    Odgaard, Lene; Nielsen, Jørgen Feldbæk; Kothari, Mohit

    Objective: To describe the prevalence of oral candidiasis (OC) in individuals with acquired brain injury (ABI) and to evaluate the association of OC with improvement in dysphagia. Design: Longitudinal observational study. Methods: Individuals with ABI admitted to a rehabilitation centre were...

  10. Katastroofide järel heldelt lubatud abi pole alati kohale jõudnud / Kaivo Kopli

    Index Scriptorium Estoniae

    Kopli, Kaivo

    2005-01-01

    ÜRO peasekretär Kofi Annan kutsus üles täitma lubadusi anda abi Lõuna-Aasia tsunamis kannatanud riikidele. Artiklis tuuakse näiteid juhtumite kohta, kus looduskatastroofi järel on riikide abi jäänud lubatust väiksemaks

  11. Molecular cloning of lupin leghemoglobin cDNA

    DEFF Research Database (Denmark)

    Konieczny, A; Jensen, E O; Marcker, K A

    1987-01-01

    Poly(A)+ RNA isolated from root nodules of yellow lupin (Lupinus luteus, var. Ventus) has been used as a template for the construction of a cDNA library. The ds cDNA was synthesized and inserted into the Hind III site of plasmid pBR 322 using synthetic Hind III linkers. Clones containing sequences...... specific for nodules were selected by differential colony hybridization using 32P-labeled cDNA synthesized either from nodule poly(A)+ RNA or from poly(A)+ RNA of uninfected root as probes. Among the recombinant plasmids, the cDNA gene for leghemoglobin was identified. The protein structure derived from...... its nucleotide sequence was consistent with known amino acid sequence of lupin Lb II. The cloned lupin Lb cDNA hybridized to poly(A)+ RNA from nodules only, which is in accordance with the general concept, that leghemoglobin is expressed exclusively in nodules. Udgivelsesdato: 1987-null...

  12. Seasonal fluctuation in concentrations and content of sugar, starch and nutrient in nordmanns fir (Abies nordmannia) Christmas tree

    DEFF Research Database (Denmark)

    Pedersen, Lars Bo; Christensen, Claus Jerram

    Seasonal fluctuation in concentrations and content of sugar, starch and nutrient in nordmanns fir (Abies nordmannia) Christmas tree......Seasonal fluctuation in concentrations and content of sugar, starch and nutrient in nordmanns fir (Abies nordmannia) Christmas tree...

  13. Bifunctional cis-Abienol Synthase from Abies balsamea Discovered by Transcriptome Sequencing and Its Implications for Diterpenoid Fragrance Production*

    Science.gov (United States)

    Zerbe, Philipp; Chiang, Angela; Yuen, Macaire; Hamberger, Björn; Hamberger, Britta; Draper, Jason A.; Britton, Robert; Bohlmann, Jörg

    2012-01-01

    The labdanoid diterpene alcohol cis-abienol is a major component of the aromatic oleoresin of balsam fir (Abies balsamea) and serves as a valuable bioproduct material for the fragrance industry. Using high-throughput 454 transcriptome sequencing and metabolite profiling of balsam fir bark tissue, we identified candidate diterpene synthase sequences for full-length cDNA cloning and functional characterization. We discovered a bifunctional class I/II cis-abienol synthase (AbCAS), along with the paralogous levopimaradiene/abietadiene synthase and isopimaradiene synthase, all of which are members of the gymnosperm-specific TPS-d subfamily. The AbCAS-catalyzed formation of cis-abienol proceeds via cyclization and hydroxylation at carbon C-8 of a postulated carbocation intermediate in the class II active site, followed by cleavage of the diphosphate group and termination of the reaction sequence without further cyclization in the class I active site. This reaction mechanism is distinct from that of synthases of the isopimaradiene- or levopimaradiene/abietadiene synthase type, which employ deprotonation reactions in the class II active site and secondary cyclizations in the class I active site, leading to tricyclic diterpenes. Comparative homology modeling suggested the active site residues Asp-348, Leu-617, Phe-696, and Gly-723 as potentially important for the specificity of AbCAS. As a class I/II bifunctional enzyme, AbCAS is a promising target for metabolic engineering of cis-abienol production. PMID:22337889

  14. [Construction of chicken embryo fibroblasts cDNA expression library].

    Science.gov (United States)

    Liu, Wei; Gao, Yu-long; Gao, Hong-lei; Wang, Xiao-mei; Xu, Xiu-hong

    2007-06-01

    Chicken embryo fibroblast (CEF) is a primary cellular material to research the infectious bursal disease virus (IBDV). Constructing the cDNA expression library of CEF is the foundation to research cell tropism and find cell receptors of IBDV from CEF. In order to achieve that purpose, a high-quality cDNA expression library of CEF was constructed by Gateway technology, which could avoid using the restriction enzyme for cloning to solve technical limitation of roution method. The mRNA was extracted from chicken embryonic fibroblast. Moreover, single-strand cDNA and double-strand cDNA were synthesized by using biotin-conjugated Oligo (dT) primer in turn. The double-strand cDNA was ligated Adapter and then purified by the cDNA Size Fractionation Columns. After BP recombination reaction, a cDNA entry library was constructed with a titer of 1 x 10(6) cfu/mL, total clones of 1.2 x 10(7) cfu and an average insertion size of about 2243 bp. After LR recombination reaction, the cDNA entry library was transformed into expression library which took on a titer of 5 x 10(5) cfu/mL, total clones of 5.5 x 10(6) cfu and an average insertion size of about 2411bp. The results indicate that the constructed cDNA expression library performs a remarkable high value in both recombination rate and library coverage. As a result, the cDNA expression library, with its good quality, may facilitate to identify the receptors associated with the resistance against IBDV in chicken embryonic fibroblast and to cast new light on the mechanism of cellular tropism. Moreover, it may also provide data of chicken embryonic fibroblast in transcription level and may be helpful to study its biological functions.

  15. [The construction of rapid amplification of cDNA ends cDNA libraries from human fetal bone and joint].

    Science.gov (United States)

    Liang, X; Gong, Y; Liu, Q; Li, J; Chen, B; Guo, C

    2001-02-01

    To construct rapid amplification cDNA ends(RACE) cDNA libraries from human fetal bone and joint and provide resources for isolation of bone- and joint-specific development-related genes. Total RNA of bone and joint were extracted with the modified single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. The double-stranded end-blunted cDNA were synthesized using TaKaRa's cDNA synthesis kit and ligated to cassette adaptors. All of the cDNA molecules were amplified by a pair of common primers. A protocol for RACE cDNA library construction from bone and joint was established and two RACE cDNA libraries from human fetal bone and joint were successfully constructed. The protocol of RACE cDNA library construction from limited materials proved to be simple and efficient and the library was suitable for RACE to isolate tissue-specific genes.

  16. Streamflow responses to afforestation with Eucalyptus grandis and Pinus patula and to felling in the Mokobulaan experimental catchments, South Africa

    CSIR Research Space (South Africa)

    Scott, DF

    1997-12-10

    Full Text Available The reductions in streamflow following the afforestation of grassland with Eucalyptus grandis and Pinus patula in the Mokobulaan research catchments on the Mpumalanga escarpment, and the subsequent response in streamflow to clearfelling...

  17. Cloning of the human androgen receptor cDNA

    International Nuclear Information System (INIS)

    Govindan, M.V.; Burelle, M.; Cantin, C.; Kabrie, C.; Labrie, F.; Lachance, Y.; Leblanc, G.; Lefebvre, C.; Patel, P.; Simard, J.

    1988-01-01

    The authors discuss how in order to define the functional domains of the human androgen receptor, complementary DNA (cDNA) clones encoding the human androgen receptor (hAR) have been isolated from a human testis λgtll cDNA library using synthetic oligonnucleotide probes, homologous to segments of the human glucocorticoid, estradiol and progesterone receptors. The cDNA clones corresponding to the human glucocorticoid, estradiol and progesterone receptors were eliminated after cross-hybridization with their respective cDNA probes and/or after restriction mapping of the cDNA clones. The remaining cDNA clones were classified into different groups after analysis by restriction digestion and cross-hybridization. Two of the largest cDNA clones from each group were inserted into an expression vector in both orientations. The linearized plasmids were used as templates in in vitro transcription with T7 RNA polymerase. Subsequent in vitro translation of the purified transcripts in rabbit reticulocyte lysate followed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) permitted the characterization of the encoded polyeptides. The expressed proteins larger than 30,000 Da were analyzed for their ability to bind tritium-labelled dihydrotestosterone ([ 3 H] DHT) with high affinity and specificity

  18. [Characteristics of Stipa grandis growth and reproductive biomass allocation at different hill position of Xilingol grassland, Inner Mongolia].

    Science.gov (United States)

    Gao, Hui; Gao, Yu-bao; Liu, Hai-ying; Liu, Jing-ling

    2009-09-01

    An investigation was conducted on the vegetative and reproductive biomass allocation of Stipa grandis at the hill-top and hill-foot of Xilingol grassland, and relatedness- and factor analyses were made to study the effects of hill position on the S. grandis seed biomass. Significant differences were observed in the vegetative and reproductive growth of S. grandis plants at the hill-top and hill-foot. At hill-foot, the height and number of non-flowering S. grandis ramets increased by 24.69% and 35.37%, respectively, the number of flowering ramets increased by 102.97%, the vegetative, reproductive, and total biomass increased by 44.14%, 95.59% and 47.45%, respectively, and the 100-grain mass increased by 25.00%, as compared with those at hill-top. The seed biomass of S. grandis at hill-top was mainly affected by soil moisture content, while that at hill-foot was mainly affected by soil pH. Hill position induced the differentiation of soil moisture and N contents, and made the S. grandis at the fertile hill-foot allocating more energy to its sexual reproduction.

  19. Cooperation of three WRKY-domain transcription factors WRKY18, WRKY40, and WRKY60 in repressing two ABA-responsive genes ABI4 and ABI5 in Arabidopsis

    OpenAIRE

    Liu, Zhi-Qiang; Yan, Lu; Wu, Zhen; Mei, Chao; Lu, Kai; Yu, Yong-Tao; Liang, Shan; Zhang, Xiao-Feng; Wang, Xiao-Fang; Zhang, Da-Peng

    2012-01-01

    Three evolutionarily closely related WRKY-domain transcription factors WRKY18, WRKY40, and WRKY60 in Arabidopsis were previously identified as negative abscisic acid (ABA) signalling regulators, of which WRKY40 regulates ABI4 and ABI5 expression, but it remains unclear whether and how the three transcription factors cooperate to regulate expression of ABI4 and ABI5. In the present experiments, it was shown that WRKY18 and WRKY60, like WRKY40, interact with the W-box in the promoters of ABI4 a...

  20. Molecular cloning of lupin leghemoglobin cDNA

    DEFF Research Database (Denmark)

    Konieczny, A; Jensen, E O; Marcker, K A

    1987-01-01

    Poly(A)+ RNA isolated from root nodules of yellow lupin (Lupinus luteus, var. Ventus) has been used as a template for the construction of a cDNA library. The ds cDNA was synthesized and inserted into the Hind III site of plasmid pBR 322 using synthetic Hind III linkers. Clones containing sequences...... its nucleotide sequence was consistent with known amino acid sequence of lupin Lb II. The cloned lupin Lb cDNA hybridized to poly(A)+ RNA from nodules only, which is in accordance with the general concept, that leghemoglobin is expressed exclusively in nodules. Udgivelsesdato: 1987-null...

  1. The Composition, Antioxidant and Antibacterial Activities of Cold-Pressed and Distilled Essential Oils of Citrus paradisi and Citrus grandis (L. Osbeck

    Directory of Open Access Journals (Sweden)

    Ming-Chiu Ou

    2015-01-01

    Full Text Available The chemical composition and functional activities of cold-pressed and water distilled peel essential oils of Citrus paradisi (C. paradisi and Citrus grandis (L. Osbeck (C. grandis were investigated in present study. Yields of cold-pressed oils were much higher than those of distilled oils. Limonene was the primary ingredient of essential oils of C. paradisi (cold 92.83%; distilled 96.06% and C. grandis (cold 32.63%; distilled 55.74%. In addition, C. grandis oils obtained were rich in oxygenated or nitrogenated compounds which may be involved in reducing cardiovascular diseases or enhancing sleep effectiveness. The order of free radical scavenging activities of 4 citrus oils was distilled C. paradisi oil > cold-pressed C. paradisi oil > distilled C. grandis oil > cold-pressed C. grandis oil. Cold-pressed C. grandis oil exhibited the lowest activity in all antioxidative assays. The order of antimicrobial activities of 4 citrus oils was distilled C. grandis oil, cold-pressed C. paradisi oil > distilled C. paradisi oil > cold-pressed C. paradisi oil. Surprisingly, distilled C. grandis oil exhibited better antimicrobial activities than distilled C. paradisi oil, especially against Escherichia coli and Salmonella enterica subsp. The results also indicated that the antimicrobial activities of essential oils may not relate to their antioxidative activities.

  2. Modification of static bending strength properties of Eucalyptus grandis heat-treated wood

    Directory of Open Access Journals (Sweden)

    Pedro Henrique Gonzalez de Cademartori

    2012-12-01

    Full Text Available The present study describes the effect of thermal rectification on physical and mechanical properties of Eucalyptus grandis wood at different levels of temperature and time. Samples of Eucalyptus grandis wood (10 × 10 × 200 mm were heat-treated at 180, 200, 220 and 240 °C during 4 and 8 hours. The mechanical properties of heat-treated and untreated samples were determined by static bending tests. The physical properties were determined by weight loss and swelling tests. The results showed that modulus of elasticity, modulus of rupture, weight loss, volumetric swelling and linear swelling were affected significantly by the thermal rectification. However, the length of exposure influenced just weight loss, while the temperature influenced all the studied properties of heat-treated wood. More significant modifications with treatments at a temperature of 200 °C or higher were found in the properties of heat-treated wood.

  3. MYCOBIOTA ASSOCIATED WITH Eucalyptus grandis HILL EX MAIDEN LUMBER DURING AIR DRYING

    Directory of Open Access Journals (Sweden)

    João Basílio Mesquita

    2010-08-01

    Full Text Available This work’s main objective was to follow the air-drying of Eucalyptus grandis lumber, carried out in Lavras, MG, and to identify the mycobiota associated with the lumber at various phases of drying. The lumbers were obtained from eight 3.0 meter long logs from 27 year-old trees, planted in an experimental area of UFLA. Results showed that the air-drying of Eucalyptus grandis, started in January, demanded 158 days to achieve moisture content around 12.5 %. The moisture content-time curve was represented by a logarithmic equation with coefficient of determination equal to 98.3 %. The highest occurrence of fungi that cause surface mold and stain was observed at the beginning of the drying. The highest frequencies of fungi were verified at the beginning of the drying for Penicillium spp and Pestalotiopsis sp. The fungus Lentinus lepideus occurred with higher frequency at the end of the drying.

  4. Assessment of the antidiabetic potential of Cassia grandis using an in vivo model

    Directory of Open Access Journals (Sweden)

    Sandesh R Lodha

    2010-01-01

    Full Text Available The aqueous and ethanolic extracts of C. grandis (Family: Leguminosae were evaluated for antidiabetic activity by a glucose tolerance test, in normal rats and alloxan-induced diabetic rats. The aqueous and ethanolic extracts showed that they significantly lowered the blood glucose levels to normal in the glucose tolerance test. In alloxan-induced diabetic rats the maximum reduction in blood glucose was observed after three hours, at a dose level of 150 mg/kg of body weight. The percentage of protection given by the aqueous and ethanolic extracts was 32.72 and 46.42%, respectively. In the long-term treatment of alloxan-induced diabetic rats, the degree of protection was determined by measuring the blood glucose, cholesterol, and triglycerides on the tenth day. Both the extracts showed a significant antidiabetic activity comparable to that of glibenclamide. These results showed that the Cassia grandis possessed significant antidiabetic activity.

  5. Callogenesis in stem explants of Eucalyptus grandis (Hill ex Maiden showing resilience in oxide calluses

    Directory of Open Access Journals (Sweden)

    Regina Paula Willemen Pereira

    Full Text Available Callogenesis was achieved using growth regulators at different concentrations to obtain the best characteristics regarding the calluses texture aspect with the main objective to produce friable calluses from stem explants of Eucalyptus grandis. Stem segments of approximately 1 cm were excised from 30-day seedlings. The treatments were prepared based on the MS medium added with TDZ alone or combined with AIA; 2,4-D; ANA. Each treatment consisted of five repetitions with five explants per plate. After 210 days of in vitro culture, the calluses were evaluated visually and by manipulation for texture. The explants that did not produce calluses were those exposed to treatments with 2,4-D 50.0 µM; TDZ 3.0 µM and in absence of a growth regulator (control.The best protocols for maintenance of friable calluses of stem explants of Eucalyptus grandis under the conditions performed in this work were those using only TDZ.

  6. Molecular cloning and characterization of a cDNA encoding ...

    African Journals Online (AJOL)

    enoh

    2012-03-29

    Nanjing) co., Ltd. The nucleotide sequences of these primers are as follows: ..... Ebizuka Y (2000). Molecular cloning and characterization of a cDNA for Glycyrrhiza glabra cycloartenol synthase. Biol. Pharm. Bull. 23(2):231-234.

  7. Method for construction of normalized cDNA libraries

    Science.gov (United States)

    Soares, Marcelo B.; Efstratiadis, Argiris

    1998-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.

  8. Screening of cDNA libraries on glass slide microarrays.

    Science.gov (United States)

    Berger, Dave K; Crampton, Bridget G; Hein, Ingo; Vos, Wiesner

    2007-01-01

    A quantitative screening method was developed to evaluate the quality of cDNA libraries constructed by suppression subtraction hybridization (SSH) or other enrichment techniques. The SSH technique was adapted to facilitate screening of the resultant library on a small number of glass slide microarrays. A simple data analysis pipeline named SSHscreen using "linear models for microarray data" (limma) functions in the R computing environment was developed to identify clones in the cDNA libraries that are significantly differentially expressed, and to determine if they were rare or abundant in the original treated sample. This approach facilitates the choice of clones from the cDNA library for further analysis, such as DNA sequencing, Northern blotting, RT-PCR, or detailed expression profiling using a custom cDNA microarray. Furthermore, this strategy is particularly useful for studies of nonmodel organisms for which there is little genome sequence information.

  9. COMPARISON BETWEEN AND WITHIN FAMILY SELECTIONS WITH THE COMBINED SELECTION IN PROGENY TESTS OF Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Ildeu Soares Martins

    2005-03-01

    Full Text Available This study used six experiments involving half-sub families of Eucalyptus grandis, which were carried out byCelulose Nipo Brasileira S. A. (CENIBRA at six locations. Data on circumference at breast height and plant height weremeasured. Selection methods among and within families were compared with combined selection. The combined selection wassuperior to selection among and within families due to higher estimates of genetic gains, and also for selecting a larger numberof families for recombination.

  10. A new species of Notiphilides from Amazonia, with a redescription of N. grandis (Chilopoda, Geophilomorpha, Oryidae).

    Science.gov (United States)

    Calvanese, Victor C; Brescovit, Antonio D

    2017-02-15

    Notiphilides grandis Brölemann, 1905 is here redescribed based on the female holotype and specimens recently collected in the type locality, Manaus, state of Amazonas, Brazil, and the male is described for the first time. Notiphilides amazonica sp. n. is described based only on female specimens from Peru and Brazil. We provide a key and distribution map for the known species of Notiphilides.

  11. A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress.

    Directory of Open Access Journals (Sweden)

    Qingqing Xie

    Full Text Available Manganese (Mn is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification.

  12. Effect of lapachol, a naphthaquinone isolated from Tectona grandis, on experimental peptic ulcer and gastric secretion.

    Science.gov (United States)

    Goel, R K; Pathak, N K; Biswas, M; Pandey, V B; Sanyal, A K

    1987-02-01

    Lapachol, a naphthaquinone isolated from the roots of Tectona grandis given at a dose of 5 mg kg-1 p.o. twice daily for 3 days was found to have an anti-ulcerogenic effect on subsequently induced experimental gastric and duodenal ulcers in rats and guinea-pigs. Its action appears to be associated with an effect on the protein content of gastric juice, and it reversed aspirin-induced changes in peptic activity, protein and sialic acid.

  13. Genetic mapping in Eucalyptus urophylla and Eucalyptus grandis using RAPD markers.

    Science.gov (United States)

    Verhaegen, D; Plomion, C

    1996-12-01

    Two single-tree linkage maps were constructed for Eucalyptus urophylla and Eucalyptus grandis, based on the segregation of 480 random amplified polymorphic DNA (RAPD) markers in a F1 interspecific progeny. A mixture of three types of single-locus segregations were observed: 244 1:1 female, 211 1:1 male, and 25 markers common to both, segregating 3:1. Markers segregating in the 1:1 ratio (testcross loci) were used to establish separate maternal and paternal maps, while markers segregating in the 3:1 ratio were used to identify homology between linkage groups of the two species maps. An average of 2.8 polymorphic loci were mapped for each arbitrary decamer primer used in the polymerase chain reaction. The mean interval size beween framework markers on the maps was 14 cM. The maps comprised 269 markers covering 1331 cM and 236 markers covering 1415 cM, in 11 linkage groups, for E. urophylla (2n = 2x = 22) and E. grandis (2n = 2x = 22), respectively. A comparative mapping analysis with two other E. urophylla and E. grandis linkage maps showed that RAPDs could be reliable markers for establishing a consensus species map. RAPD markers were automatically and quantitatively scored with an imaging analyzer. They were classified into four categories based on their optical density. A fragment intensity threshold is proposed to optimize the selection of reliable RAPD markers for future mapping experiments. Key words : genetic linkage map, Eucalyptus urophylla, Eucalyptus grandis, random amplified polymorphic DNA, RAPD, automated data collection.

  14. A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress.

    Science.gov (United States)

    Xie, Qingqing; Li, Zhenji; Yang, Limin; Lv, Jing; Jobe, Timothy O; Wang, Qiuquan

    2015-01-01

    Manganese (Mn) is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM) X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry) lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification.

  15. The oropharyngeal morphology in the semiaquatic giant Asian pond turtle, Heosemys grandis, and its evolutionary implications.

    Directory of Open Access Journals (Sweden)

    Monika Lintner

    Full Text Available The oropharynx as a functional entity plays a fundamental role in feeding. Transitions from aquatic to terrestrial lifestyles in vertebrates demanded major changes of the oropharynx for the required adaptations to a different feeding environment. Extant turtles evolved terrestrial feeding modes in three families (testudinids, emydids, geoemydids-independently from other amniotes-and are therefore important model organisms to reconstruct morpho-functional changes behind aquatic-terrestrial transitions. In this study we hypothesized that the oropharyngeal morphology in semiaquatic turtles of the geoemydid family shows parallels to testudinids, the only purely terrestrial extant lineage. We provide an in-depth description of the oropharynx in the semiaquatic geoemydid Heosemys grandis by using a combination of micro computed tomography (micro-CT and subsequent digital in situ 3-D reconstruction, scanning electron microscopy (SEM, and histology. We show that H. grandis has a large tongue with rough papillose surface and well-developed lingual muscles. The attachment sites of the lingual muscles on the hyolingual skeleton and their courses within the tongue are nearly identical with testudinids. The hyolingual skeleton itself is mainly cartilaginous and shows distinct-but compared to testudinids rather small-anterior extensions of the hyoid body and hypoglossum. Oral glands are well developed in H. grandis but are smaller and simpler than in testudinids. Similarly, oropharyngeal keratinization was minimal and found only in the anterior palate, regions close to the beak, and tongue tip. We conclude that H. grandis shows distinct oropharyngeal morpho-functional adaptations for a terrestrial lifestyle but still retains characters typical for aquatic forms. This makes this species an important example showing the oropharyngeal adaptations behind aquatic-terrestrial transitions in turtles.

  16. Constructing and detecting a cDNA library for mites.

    Science.gov (United States)

    Hu, Li; Zhao, YaE; Cheng, Juan; Yang, YuanJun; Li, Chen; Lu, ZhaoHui

    2015-10-01

    RNA extraction and construction of complementary DNA (cDNA) library for mites have been quite challenging due to difficulties in acquiring tiny living mites and breaking their hard chitin. The present study is to explore a better method to construct cDNA library for mites that will lay the foundation on transcriptome and molecular pathogenesis research. We selected Psoroptes cuniculi as an experimental subject and took the following steps to construct and verify cDNA library. First, we combined liquid nitrogen grinding with TRIzol for total RNA extraction. Then, switching mechanism at 5' end of the RNA transcript (SMART) technique was used to construct full-length cDNA library. To evaluate the quality of cDNA library, the library titer and recombination rate were calculated. The reliability of cDNA library was detected by sequencing and analyzing positive clones and genes amplified by specific primers. The results showed that the RNA concentration was 836 ng/μl and the absorbance ratio at 260/280 nm was 1.82. The library titer was 5.31 × 10(5) plaque-forming unit (PFU)/ml and the recombination rate was 98.21%, indicating that the library was of good quality. In the 33 expressed sequence tags (ESTs) of P. cuniculi, two clones of 1656 and 1658 bp were almost identical with only three variable sites detected, which had an identity of 99.63% with that of Psoroptes ovis, indicating that the cDNA library was reliable. Further detection by specific primers demonstrated that the 553-bp Pso c II gene sequences of P. cuniculi had an identity of 98.56% with those of P. ovis, confirming that the cDNA library was not only reliable but also feasible.

  17. EFFECT OF FERTILIZATION ON MECHANICAL PROPERTIES OF THE WOOD OF Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Israel Luiz de Lima

    2011-09-01

    Full Text Available The effect of the fertilization in the amount and quality of the produced wood is one of the questions to be considered in the research of the Eucalyptus grandis. The present work aimed to evaluate the fertilization effect in the mechanical properties of Eucalyptus grandis. The population of Eucalyptus grandis was 21 years old and was managed under the system of selective thinning, with application of fertilizers. The factors used in this study were: presence or absence of fertilizers, two positions of log and five radial positions. The influences of the factors and of their combinations were evaluated regarding to compression strength, shear strength, modulus of rupture and modulus of elasticity in static banding. The compressive strength and the modulus of elasticity had been influenced by the factors: fertilizer and radial positions of the log. There was also an increase in the direction of the pith-bark in all studied properties. A good positive relationship was found to exist among the compression strength, the shear, the modulus of rupture and the modulus of elasticity with radial position.

  18. Evaluation of Chemical Composition and Biological Activities of Citrus pseudolimon and Citrus grandis Peel Essential Oils

    International Nuclear Information System (INIS)

    Sajid, A.; Hanif, M.A.; Shahid, M.

    2016-01-01

    Essential oils and their volatile constituents are used extensively to prevent and treat human diseases. In the past decades, worldwide demand for citrus essential oils has greatly increased. Citrus essential oils containing 85-99 percent volatile and 1-15 percent non-volatile components. Essential oils from Citrus pseudolimon and Citrus grandis peels were extracted through steam distillation and characterized by GC-MS. C. pseudolimon has thirty six and C. grandis has thirty three total components; limonene 47.07 percent and 71.48 percent was the major component in both oils respectively. Antioxidant activity was checked by 2, 2-diphenyl-1-picrylhydrazyl radical assay and β-carotene/linoleic acid bleaching test. Both oils have modest activity. The antimicrobial potential was assessed against different bacterial and fungus strains. C. pseudolimon oil possessed strong activity against all tested strains while C. grandis has moderate activity. The antitumor activity was evaluated by potato disc assay, C. pseudolimon showed 81.25 inhibition. Hence the essential oils could have a great potential in pharmaceutical industry. (author)

  19. Combining ability of elite clones of Eucalyptus grandis and Eucalyptus urophylla with Eucalyptus globulus

    Directory of Open Access Journals (Sweden)

    Odair Bison

    2007-03-01

    Full Text Available In Brazil, eucalyptus breeding programs for cellulose production has used two species, Eucalyptus grandis and Eucalyptus urophylla. Nevertheless, it would be useful to introgress alleles from other species to improve wood quality and volume. The objective of this research was to evaluate the hybrid potential of elite clones of E. grandis and E. urophylla from the Aracruz Celulose Company S. A. with Eucalyptus globulus clones. To do so, six elite clones were crossed with ten E. globulus clones in a half-diallel mating design. The resulting hybrid combinations as well as the four check clones were evaluated in randomized complete block experiments with single plant plots and 40 replicates from September to October 2001 at three Brazilian sites, Aracruz and São Mateus in the Espírito Santo state and Caravelas in Bahia State. Two years later the circumference at breast height (CBH and the wood density (WD were measured. The means were submitted to diallel analysis according to the Griffing method (1956, adapted by Geraldi and Miranda Filho (1988. Although the number of clones involved was small, the crossings of elite clones of E. grandis and E. urophylla with clones of E. globulus were promising, especially for wood quality gains.

  20. Development and assessment of microarray-based DNA fingerprinting in Eucalyptus grandis.

    Science.gov (United States)

    Lezar, Sabine; Myburg, A A; Berger, D K; Wingfield, M J; Wingfield, B D

    2004-11-01

    Development of improved Eucalyptus genotypes involves the routine identification of breeding stock and superior clones. Currently, microsatellites and random amplified polymorphic DNA markers are the most widely used DNA-based techniques for fingerprinting of these trees. While these techniques have provided rapid and powerful fingerprinting assays, they are constrained by their reliance on gel or capillary electrophoresis, and therefore, relatively low throughput of fragment analysis. In contrast, recently developed microarray technology holds the promise of parallel analysis of thousands of markers in plant genomes. The aim of this study was to develop a DNA fingerprinting chip for Eucalyptus grandis and to investigate its usefulness for fingerprinting of eucalypt trees. A prototype chip was prepared using a partial genomic library from total genomic DNA of 23 E. grandis trees, of which 22 were full siblings. A total of 384 cloned genomic fragments were individually amplified and arrayed onto glass slides. DNA fingerprints were obtained for 17 individuals by hybridizing labeled genome representations of the individual trees to the 384-element chip. Polymorphic DNA fragments were identified by evaluating the binary distribution of their background-corrected signal intensities across full-sib individuals. Among 384 DNA fragments on the chip, 104 (27%) were found to be polymorphic. Hybridization of these polymorphic fragments was highly repeatable (R2>0.91) within the E. grandis individuals, and they allowed us to identify all 17 full-sib individuals. Our results suggest that DNA microarrays can be used to effectively fingerprint large numbers of closely related Eucalyptus trees.

  1. Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis

    Directory of Open Access Journals (Sweden)

    Gomes José E

    2011-09-01

    Full Text Available Abstract Background The cotton boll weevil (Anthonomus grandis is a serious insect-pest in the Americas, particularly in Brazil. The use of chemical or biological insect control is not effective against the cotton boll weevil because of its endophytic life style. Therefore, the use of biotechnological tools to produce insect-resistant transgenic plants represents an important strategy to reduce the damage to cotton plants caused by the boll weevil. The present study focuses on the identification of novel molecules that show improved toxicity against the cotton boll weevil. In vitro directed molecular evolution through DNA shuffling and phage display screening was applied to enhance the insecticidal activity of variants of the Cry8Ka1 protein of Bacillus thuringiensis. Results Bioassays carried out with A. grandis larvae revealed that the LC50 of the screened mutant Cry8Ka5 toxin was 3.15-fold higher than the wild-type Cry8Ka1 toxin. Homology modelling of Cry8Ka1 and the Cry8Ka5 mutant suggested that both proteins retained the typical three-domain Cry family structure. The mutated residues were located mostly in loops and appeared unlikely to interfere with molecular stability. Conclusions The improved toxicity of the Cry8Ka5 mutant obtained in this study will allow the generation of a transgenic cotton event with improved potential to control A. grandis.

  2. Gas Exchange Characteristics in Tectona grandis L. Clones under Varying Concentrations of CO2 Levels

    Directory of Open Access Journals (Sweden)

    S. Saravanan

    2014-08-01

    Full Text Available The Institute of Forest Genetics and Tree Breeding, Coimbatore, India functioning under the Indian Council of Forestry Research and Education, Dehara Dun, has a long term systematic tree improvement program for Tectona grandis aimed to enhancing productivity and screening of clones for site specific. In the process, twenty clones of T. grandis L. were studied for the physiological parameters and water use efficiency with reference to the elevated CO2 levels. CO2 enrichment studies in special chambers help in understanding the changes at individual level, and also at physiological, biochemical and genetic level. It also provides valuable information for establishing plantations at different geographic locations. Considerable variations were observed when the selected 20 clones of T. grandis were subjected to physiological studies under elevated CO2 conditions (600 and 900 mol mol-1. Eight clones exhibited superior growth coupled with favorable physiological characteristics including high photosynthetic rate, carboxylation and water use efficiency under elevated CO2 levels. Clones with minimal variation in physiological characteristics under elevated levels of CO2 suggest their ability to overcome physiological stresses and adapt to varying climatic conditions.

  3. High-Throughput Plasmid cDNA Library Screening

    Energy Technology Data Exchange (ETDEWEB)

    Wan, Kenneth H.; Yu, Charles; George, Reed A.; Carlson, JosephW.; Hoskins, Roger A.; Svirskas, Robert; Stapleton, Mark; Celniker, SusanE.

    2006-05-24

    Libraries of cDNA clones are valuable resources foranalysing the expression, structure, and regulation of genes, as well asfor studying protein functions and interactions. Full-length cDNA clonesprovide information about intron and exon structures, splice junctionsand 5'- and 3'-untranslated regions (UTRs). Open reading frames (ORFs)derived from cDNA clones can be used to generate constructs allowingexpression of native proteins and N- or C-terminally tagged proteins.Thus, obtaining full-length cDNA clones and sequences for most or allgenes in an organism is critical for understanding genome functions.Expressed sequence tag (EST) sequencing samples cDNA libraries at random,which is most useful at the beginning of large-scale screening projects.However, as projects progress towards completion, the probability ofidentifying unique cDNAs via EST sequencing diminishes, resulting in poorrecovery of rare transcripts. We describe an adapted, high-throughputprotocol intended for recovery of specific, full-length clones fromplasmid cDNA libraries in five days.

  4. Cloning of the cDNA for human 12-lipoxygenase

    International Nuclear Information System (INIS)

    Izumi, T.; Hoshiko, S.; Radmark, O.; Samuelsson, B.

    1990-01-01

    A full-length cDNA clone encoding 12-lipoxygenase was isolated from a human platelet cDNA library by using a cDNA for human reticulocyte 15-lipoxygenase as probe for the initial screening. The cDNA had an open reading frame encoding 662 amino acid residues with a calculated molecular weight of 75,590. Three independent clones revealed minor heterogeneities in their DNA sequences. Thus, in three positions of the deduced amino acid sequence, there is a choice between two different amino acids. The deduced sequence from the clone plT3 showed 65% identity with human reticulocyte 15-lipoxygenase and 42% identity with human leukocyte 5-lipoxygenase. The 12-lipoxygenase cDNA recognized a 3.0-kilobase mRNA species in platelets and human erythroleukemia cells (HEL cells). Phorbol 12-tetradecanoyl 13-acetate induced megakaryocytic differentiation of HEL cells and 12-lipoxygenase activity and increased mRNA for 12-lipoxygenase. The identity of the cloned 12-lipoxygenase was assured by expression in a mammalian cell line (COS cells). Human platelet 12-lipoxygenase has been difficult to purify to homogeneity. The cloning of this cDNA will increase the possibilities to elucidate the structure and function of this enzyme

  5. Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination.

    Science.gov (United States)

    Bi, Chao; Ma, Yu; Wu, Zhen; Yu, Yong-Tao; Liang, Shan; Lu, Kai; Wang, Xiao-Fang

    2017-05-01

    It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines. Compared with Col-0, the seeds of abi5 mutants showed more sensitive to 3-AT during seed germination, while the seeds of ABI5-overexpression transgenic lines showed more insensitive. H 2 O 2 showed the same effect on seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines as 3-AT. These results suggest that ROS is involved in the seed germination mediated by ABI5. Further, we observed that T-DNA insertion mutants of the three catalase members in Arabidopsis displayed 3-AT-insensitive or -hypersensitive phenotypes during seed germination, suggesting that these catalase members regulate ROS homeostasis in a highly complex way. ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASE expression and catalase activity. Furthermore, we showed that ABI5 directly binds to the CAT1 promoter and activates CAT1 expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. RNA-sequencing analysis indicates that the transcription of the genes involved in ROS metabolic process or genes responsive to ROS stress is impaired in abi5-1 seeds. Additionally, expression changes in some genes correlative to seed germination were showed due to the change in ABI5 expression under 3-AT treatment. Together, all the findings suggest that ABI5 regulates seed germination at least partly by affecting ROS homeostasis.

  6. The ABA-INSENSITIVE-4 (ABI4) transcription factor links redox, hormone and sugar signaling pathways.

    Science.gov (United States)

    Foyer, Christine H; Kerchev, Pavel I; Hancock, Robert D

    2012-02-01

    The cellular reduction-oxidation (redox) hub processes information from metabolism and the environment and so regulates plant growth and defense through integration with the hormone signaling network. One key pathway of redox control involves interactions with ABSCISIC ACID (ABA). Accumulating evidence suggests that the ABA-INSENSITIVE-4 (ABI4) transcription factor plays a key role in transmitting information concerning the abundance of ascorbate and hence the ability of cells to buffer oxidative challenges. ABI4 is required for the ascorbate-dependent control of growth, a process that involves enhancement of salicylic acid (SA) signaling and inhibition of jasmonic acid (JA) signaling pathways. Low redox buffering capacity reinforces SA- JA- interactions through the mediation of ABA and ABI4 to fine-tune plant growth and defense in relation to metabolic cues and environmental challenges. Moreover, ABI4-mediated pathways of sugar sensitivity are also responsive to the abundance of ascorbate, providing evidence of overlap between redox and sugar signaling pathways.

  7. Portugal palus ELilt abi põlengutega võitlemiseks / Kajar Kase

    Index Scriptorium Estoniae

    Kase, Kajar

    2005-01-01

    Sellel aastal on Portugali metsatulekahjudes hukkunud juba 15 inimest ja hetkel on kahjustatud 140 000 hektarit metsa. Appi on tõtanud Prantsusmaa, Saksamaa, Itaalia, Hispaania ja Hollandi helikopterid ja lennukid. Kaart: Abi Portugalile

  8. NOAA GOES-R Series Advanced Baseline Imager (ABI) Level 1b Radiances

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Advanced Baseline Imager (ABI) instrument samples the radiance of the Earth in sixteen spectral bands using several arrays of detectors in the instrument’s...

  9. Genome-wide mapping of histone H3 lysine 4 trimethylation in Eucalyptus grandis developing xylem.

    Science.gov (United States)

    Hussey, Steven G; Mizrachi, Eshchar; Groover, Andrew; Berger, Dave K; Myburg, Alexander A

    2015-05-10

    Histone modifications play an integral role in plant development, but have been poorly studied in woody plants. Investigating chromatin organization in wood-forming tissue and its role in regulating gene expression allows us to understand the mechanisms underlying cellular differentiation during xylogenesis (wood formation) and identify novel functional regions in plant genomes. However, woody tissue poses unique challenges for using high-throughput chromatin immunoprecipitation (ChIP) techniques for studying genome-wide histone modifications in vivo. We investigated the role of the modified histone H3K4me3 (trimethylated lysine 4 of histone H3) in gene expression during the early stages of wood formation using ChIP-seq in Eucalyptus grandis, a woody biomass model. Plant chromatin fixation and isolation protocols were optimized for developing xylem tissue collected from field-grown E. grandis trees. A "nano-ChIP-seq" procedure was employed for ChIP DNA amplification. Over 9 million H3K4me3 ChIP-seq and 18 million control paired-end reads were mapped to the E. grandis reference genome for peak-calling using Model-based Analysis of ChIP-Seq. The 12,177 significant H3K4me3 peaks identified covered ~1.5% of the genome and overlapped some 9,623 protein-coding genes and 38 noncoding RNAs. H3K4me3 library coverage, peaking ~600 - 700 bp downstream of the transcription start site, was highly correlated with gene expression levels measured with RNA-seq. Overall, H3K4me3-enriched genes tended to be less tissue-specific than unenriched genes and were overrepresented for general cellular metabolism and development gene ontology terms. Relative expression of H3K4me3-enriched genes in developing secondary xylem was higher than unenriched genes, however, and highly expressed secondary cell wall-related genes were enriched for H3K4me3 as validated using ChIP-qPCR. In this first genome-wide analysis of a modified histone in a woody tissue, we optimized a ChIP-seq procedure suitable

  10. Lignin distribution in waterlogged archaeological Picea abies (L.) Karst degraded by erosion bacteria

    DEFF Research Database (Denmark)

    Pedersen, Nanna Bjerregaard; Schmitt, Uwe Schmitt; Koch, Gerald

    2014-01-01

    The lignin distribution in poles of waterlogged archaeological Picea abies (L.) Karst, which was decayed by erosion bacteria (EB) under anoxic conditions for approximately 400 years, was topochemically identified by transmission electron microscopy (TEM) and high resolution UV-microspectrophotome......The lignin distribution in poles of waterlogged archaeological Picea abies (L.) Karst, which was decayed by erosion bacteria (EB) under anoxic conditions for approximately 400 years, was topochemically identified by transmission electron microscopy (TEM) and high resolution UV...

  11. Comparisons between two economically valuable forest species (Eucalyptus grandis and Pinus taeda in relation to seed behaviour under controlled deterioration

    Directory of Open Access Journals (Sweden)

    Jussara Bertho Fantinatti

    2011-02-01

    Full Text Available The objectives of this work were to analyze seed behaviour under controlled deterioration and estimate viability equations for forest species Eucalyptus grandis and Pinus taeda. Desired moisture content levels were achieved from initial values after either rehydration over water or drying over silica gel, both at 25 ºC. Seed sub samples with 8 moisture contents each for E. grandis (1.2 to 18.1%, initial value of 11.3% and P. taeda (1.5 to 19.5%, initial value of 12.9% were sealed in laminate aluminium-foil packets and stored in incubators maintained at 40, 50 and 65 ºC. The seeds from these species exhibited true orthodox and sub-orthodox storage behaviour, respectively, however E. grandis showed higher seed storability, probably due to a different seed chemical composition. Lowest moisture content limits estimated for application of the viability equations at 65 ºC were 4.9 and 4.1 mc for E. grandis and P. taeda, on equilibrium with ±20% RH. The viability equation estimated quantified the response of seed longevity to storage environment well with K E = 9.661 and 8.838; C W = 6.467 and 5.981; C H = 0.03498 and 0.10340; C Q = 0.0002330 and 0.0005476, for E. grandis and P. taeda, respectively.

  12. MODIFICACIÓN AL PROCESO DE PRODUCCIÓN MASIVA DE LOS PARASITOIDES Catolaccus grandis Y Catolaccus hunteri

    Directory of Open Access Journals (Sweden)

    Marco Antonio Reyes-Rosas

    2010-01-01

    Full Text Available El presente estudio se realizó con el objetivo de modificar la tecnología de producción masiva de Catolaccus grandis y Catolaccus hunteri, himenópteros parasitoides de Anthonomus grandis el picudo del algodonero en Tamaulipas, México en el 2002. Se determinó la eficiencia del papel toalla para el secado de manos, en el sellamiento de cápsulas moldeadas en cera de hidrocarburos y poliolefina (LCHP, donde se confina al huésped, y evaluar la efectividad del gorgojo del garbanzo Callosobruchus maculatus como hospedante facticio para la cría de ambos parasitoides. Hubo producción masiva de C. grandis y C. hunteri utilizando C. maculatus como huésped y el papel toalla fue efectivo para sellar los orificios de las cápsulas moldeadas en LCHP. La producción masiva de C. grandis y C. hunteri mediante C. maculatus, contribuyó a incrementar la seguridad y facilidad del proceso, además de una significativa reducción de costos. El papel toalla en el sellado de los orificios de las cápsulas moldeadas en LCHP, redujo en un 50% por m2 los costos del proceso, sin afectar la eficiencia del parasitismo y emergencia de C. grandis.

  13. cDNA library Table - KAIKOcDNA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us KAIKOcDNA... cDNA library Table Data detail Data name cDNA library Table DOI 10.18908/lsdba.nbd...c00951-005 Description of data contents List of Bombyx mori cDNA libraries. Data file File name: kaiko_cdna_...iption Registered library name Registered name of the partial cDNA library Library synonym Another name for cDNA... Download License Update History of This Database Site Policy | Contact Us cDNA library Table - KAIKOcDNA | LSDB Archive ...

  14. Study on construction of cDNA library of the treated changliver cell and quality analysis

    OpenAIRE

    Juntang, Lin; Pramanik, Jogenananda; Congrui, Wang; Huiyong, Zhang; Huigen, Feng; Baosheng, Yang; Yuchang, Li; Cunshuan, Xu

    2004-01-01

    The study aims to construct cDNA library of Changliver cell by SMART (switching mechanism at 5′ end of RNA transcript) technique and analyze its quality. cDNA of Changliver cell was made with RT-PCR and LD-PCR (long-distance PCR), the cDNA library was constructed with SMART cDNA library construction kit. Through testing, the high quality cDNA library containing whole long cDNA of Changliver cell had been constructed. The titer of the amplified cDNA library was 4.5 × 1010 pfu/ml and the averag...

  15. cDNA library construction of two human Demodexspecies.

    Science.gov (United States)

    Niu, DongLing; Wang, RuiLing; Zhao, YaE; Yang, Rui; Hu, Li; Lei, YuYang; Dan, WeiChao

    2017-06-01

    The research of Demodex, a type of pathogen causing various dermatoses in animals and human beings, is lacking at RNA level. This study aims at extracting RNA and constructing cDNA library for Demodex. First, P. cuniculiand D. farinaewere mixed to establish homogenization method for RNA extraction. Second, D. folliculorumand D. breviswere collected and preserved in Trizol, which were mixed with D. farinaerespectively to extract RNA. Finally, cDNA library was constructed and its quality was assessed. The results indicated that for D. folliculorum& D. farinae, the recombination rate of cDNA library was 90.67% and the library titer was 7.50 × 104 pfu/ml. 17 of the 59 positive clones were predicted to be of D. folliculorum; For D. brevis& D. farinae, the recombination rate was 90.96% and the library titer was 7.85 x104 pfu/ml. 40 of the 59 positive clones were predicted to be of D. brevis. Further detection by specific primers demonstrated that mtDNA cox1, cox3and ATP6 detected from cDNA libraries had 96.52%-99.73% identities with the corresponding sequences in GenBank. In conclusion, the cDNA libraries constructed for Demodexmixed with D. farinaewere successful and could satisfy the requirements for functional genes detection.

  16. Second-strand cDNA synthesis: classical method

    International Nuclear Information System (INIS)

    Gubler, U.

    1987-01-01

    The classical scheme for the synthesis of double-stranded cDNA as it was reported in 1976 is described. Reverse transcription of mRNA with oligo(dT) as the primer generates first strands with a small loop at the 3' end of the cDNA (the end that corresponds to the 5' end of the mRNA). Subsequent removal of the mRNA by alkaline hydrolysis leaves single-stranded cDNA molecules again with a small 3' loop. This loop can be used by either reverse transcriptase or Klenow fragment of DNA polymerase I as a primer for second-strand synthesis. The resulting products are double-stranded cDNA molecules that are covalently closed at the end corresponding to the 5' end of the original mRNA. Subsequent cleavage of the short piece of single-stranded cDNA within the loop with the single-strand-specific S 1 nuclease generate open double-stranded molecules that can be used for molecular cloning in plasmids or in phage. Useful variations of this scheme have been described

  17. GPR survey for fir (Abies alba) and spruce (Picea abies) root systems in different location in Western Carpathians Mts. (Beskidy Mts., Poland)

    Science.gov (United States)

    Szynkiewicz, Adam

    2017-04-01

    The aim of the GPR research was a non-invasive inspection of the root systems arrangement of selected trees (fir Abies alba and spruce Picea abies), in the Beskid Śląski Landscaped Park and Żywiecki Landscaped Park (Carpathian Mountains, Poland). Field research has been done using RAMAC/GPR with 800 MHz shielded antennas. The survey was conducted by linear profiling to a depth of 2 m. The survey was carried out around the designated trees in 6 meters x 6 meters grids. Base points for X (S-N) and Y (W-E) axis were set in corners of each grid. Parallel GPR traverses were conducted within each study area, at intervals of 0.20 m. The maps of research areas show GPR sections, with + 0.1 m error and the other existing trees and stumps. GPR data analysis was carried out in 2D and 3D system. The survey result in the following conclusions: 1) fir (Abies alba), has a "vertical" root system type (withthe roots dominance at depths of 0.2 - 0.8 meter), concentrically away from the tree trunk at a distance of about 1 m to about 2 m. 2) spruce (Picea abies), has a "cloud" root systems type (at a depth of 10 - 100 cm), with a few clear, thicker roots extending from the trunk.

  18. Tolerance of Anadenanthera peregrina to Eucalyptus camaldulensis and Eucalyptus grandis essential oil as condition for mixed plantation

    Directory of Open Access Journals (Sweden)

    Neimar de Freitas Duarte

    2012-06-01

    Full Text Available With the purpose of selecting the species of woody Caatinga for mixed plantations with Eucalyptus spp., the allelophatic effects of E. camaldulensis and E. grandis essential oil were studied on the growth activities of Anadenanthera peregrina. The plants were closed in glass chambers in the presence of volatile oil of E. camaldulensis or E. grandis at the concentration of 13 nl.cm-3. The number of leaves, height and diameter at soil lever were compared before, immediately after and after 30 days. Chlorophyll a and b, carotenoids and dry mass were evaluated after the treatment application. There was no inhibitory effect of E. camaldulensis and E. grandis oils on A. peregrina. E. camaldulensis, which was more adapted to semi-arid conditions, was planted in mixture stands with two native legume species, inoculated with Rhizobium and arbuscular mycorrhizal fungi. E. camaldulensis did not inhibit native species growth after two years of cultivation.

  19. cDNA libraries for virus-induced gene silencing.

    Science.gov (United States)

    Todd, Andrea T; Liu, Enwu; Page, Jonathan E

    2010-01-01

    Virus-induced gene silencing (VIGS) exploits endogenous plant antiviral defense mechanisms to posttranscriptionally silence the expression of targeted plant genes. VIGS is quick and relatively easy to perform and therefore serves as a powerful tool for high-throughput functional genomics in plants. Combined with the use of subtractive cDNA libraries for generating a collection of VIGS-ready cDNA inserts, VIGS can be utilized to screen a large number of genes to determine phenotypes resulting from the knockdown/knockout of gene function. Taking into account the optimal insert design for VIGS, we describe a methodology for producing VIGS-ready cDNA libraries enriched for inserts relevant to the biological process of interest.

  20. Procedure for normalization of cDNA libraries

    Science.gov (United States)

    Bonaldo, Maria DeFatima; Soares, Marcelo Bento

    1997-01-01

    This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.

  1. Correlações entre caracteres silvicuturais durante o crescimento de Eucalyptus grandis Hill ex Maiden. Correlations between forestry characters during Eucalyptus grandis Hill ex Maiden growth.

    Directory of Open Access Journals (Sweden)

    Léo ZIMBACK

    2011-06-01

    Full Text Available A espécie Eucalyptus grandis é a mais comumente cultivada em estandes comerciais no Brasil e no mundo. A espécie é plantada como cultivar e também na forma de plantios clonais de seus híbridos interespecíficos. Este trabalho é um estudo da variabilidade genética em progênies de polinização livre de Eucalyptus grandis (Hill Maiden, oriundas de pomar de sementes por muda, cuja procedência é originária de Coff’s Harbour – Austrália, de propriedade da empresa Duratex S.A., localizado na Fazenda Morro D’Ouro, no município de Botucatu. Teve como objetivo analisar as correlações entre diferentes caracteres nas diferentes idades, visando subsidiar a seleção precoce em programas de melhoramento para essa espécie. Os ensaios foram instalados em dois locais, Angatuba–SP e Lençóis Paulista–SP, em fevereiro de 1988. O delineamento experimental utilizado foi o de blocos casualizados, com dez repetições, seis plantas por parcela, ao espaçamento de 3 x 2 m e totalizando 76 progênies. Os caracteres avaliados foram: (1 diâmetro à altura do peito – (DAP; altura total e volume de madeira. As avaliações foram feitas em quatro anos iniciando a partir do segundo ano em Lençóis Paulista (local 2 até o quinto ano, sendo que em Angatuba (local 1 foram realizadas avaliações anuais do segundo ao sexto ano. Os resultados mostraram que as correlações entre os caracteres de crescimento apresentaram valores elevados (0,69 a 0,99, tanto genéticos como fenotípicos, facilitando os procedimentos de seleção no avanço das gerações de melhoramento, podendo ser realizada desde o terceiro ano de idade com maior peso para DAP e volume em um índice de seleção.The species Eucalyptus grandis (Hill Maiden is the most commercial tree cultivated in Brazil and throughout the world. It is planted as varieties and also as interspecific hybrid clonal plantings. This work is a study of genetic variation in open pollinated progenies, a

  2. Identification of molecular markers linked to early flowering in Eucalyptus grandis. Identificação de marcadores moleculares relacionados ao florescimento precoce em Eucalyptus grandis.

    Directory of Open Access Journals (Sweden)

    Ana Paula Cazerta FARRO

    2012-12-01

    Full Text Available Flowering time is an important trait for tree breeding because it determines the speed of generation turnover and therefore the rapidity of genetic gains, and it is of particular interest in Eucalyptus species. In this work, we used simple sequence repeats (SSRs, random amplified polymorphic DNA (RAPD analysis and specific markers for flowering to evaluate early flowering segregation in a full-sibling family of Eucalyptus grandis and to identify molecular markers associated with the control of flowering time. A cross between a normal flowering tree (wild-type and early flowering tree resulted in 118 progeny with a 1:1 Mendelian segregation ratio for flowering time (χ2 = 0.5424, P > 0.05, which suggested the action of one main gene in a locus named Early flowering in Eucalyptus grandis (PFEg. The SSR marker EMBRA 02 was related to the QTL PFEg, and identified this region as a candidate for trait control. These maps may be used as the basis for a study in which can be inserted new markers in an attempt to find more loci related to early flowering characteristic on eucalyptus. O tempo de florescimento é uma importante característica para o melhoramento genético de árvores, pois este determina o prazo para o surgimento de uma nova geração e a rapidez para o ganho genético de uma determinada espécie, como por exemplo, Eucalyptusgrandis. Neste estudo foram utilizados marcadores moleculares do tipo microssatélites, Random Amplified Polymorphism DNA – RAPD, além de marcadores específicos para avaliar a segregação do florescimento precoce em uma família de irmãos-germanos de E. grandis. Foi também verificado se esses marcadores estão associados ao controle do tempo de florescimento na espécie. A progênie de 118 indivíduos avaliada foi originada do cruzamento entre uma árvore de florescimento normal e outra de florescimento precoce. A segregação da característica de florescimento precoce apresentou uma razão de 1:1 (χ2 = 0

  3. Restriction landmark cDNA scanning (RLCS): a novel cDNA display system using two-dimensional gel electrophoresis.

    Science.gov (United States)

    Suzuki, H; Yaoi, T; Kawai, J; Hara, A; Kuwajima, G; Wantanabe, S

    1996-01-01

    We have developed a new method, designated restriction landmark cDNA scanning (RLCS), which displays many cDNA species quantitatively and simultaneously as two-dimensional gel spots. In this method cDNA species of uniform length were prepared for each mRNA species using restriction enzymes. After the restriction enzyme sites were radiolabeled as landmarks, the labeled fragments were subjected to high resolution two-dimensional gel electrophoresis. In analyses of cDNA samples from adult mouse liver and brain (cerebral cortex, cerebellum and brain stem) we detected approximately 500 and >1000 discrete gel spots respectively of various intensities at a time. The spot patterns of the three brain regions were very similar, although not identical, but were quite different from the pattern for the liver. RNA blot hybridization analysis using several cloned spot DNAs as probes showed that differences in intensity of the spots among RLCS profiles correlated well with expression levels of the corresponding mRNA species in the brain regions. Because the spots and their intensities reflect distinct mRNA species and their expression level respectively, the RLCS is a novel cDNA display system which provides a great deal of information and should be useful for systematic documentation of differentially expressed genes. PMID:8628652

  4. Delphinella Shoot Blight on Abies lasiocarpa Provenances in Norway

    Directory of Open Access Journals (Sweden)

    Venche Talgø

    2015-12-01

    Full Text Available Delphinella shoot blight (Delphinella abietis attacks true firs (Abies spp. in Europe and North America. Especially subalpine fir (A. lasiocarpa, one of the main Christmas tree species in Norway, is prone to the disease. The fungus kills current year needles, and in severe cases entire shoots. Dead needles become covered with black fruiting bodies, both pycnidia and pseudothecia. Delphinella shoot blight has mainly been a problem in humid, coastal regions in the northwestern part of Southern Norway, but, probably due to higher precipitation in inland regions during recent years, heavy attacks were found in 2011 in a field trial with 76 provenances of subalpine fir in Southeastern Norway. However, the amount of precipitation seemed less important once the disease had established in the field. Significant differences in susceptibility between provenances were observed. In general, the more bluish the foliage was, the healthier the trees appeared. The analysis of provenance means indicated that, at least for the southern range, the disease ratings were correlated with foliage color. This study also includes isolation, identification, a pathogenicity test, a seed test and electron microscopy of the wax layer on the needles. The fungus was identified based on the morphology of spores and by sequencing the Internal Transcribed Spacer (ITS regions of the ribosomal DNA. Koch’s postulates were fulfilled. The fungus was found present on newly harvested seeds and may therefore spread via international seed trade. When comparing the wax layers on green and blue needles, those of the latter were significantly thicker, a factor that may be involved in disease resistance.

  5. Mean canopy stomatal conductance responses to water and nutrient availabilities in Picea abies and Pinus taeda.

    Science.gov (United States)

    Ewers, B E; Oren, R; Phillips, N; Strömgren, M; Linder, S

    2001-08-01

    We compared sap-flux-scaled, mean, canopy stomatal conductance (GS) between Picea abies (L.) Karst. in Sweden and Pinus taeda (L.) in North Carolina, both growing on nutritionally poor soils. Stomatal conductance of Picea abies was approximately half that of Pinus taeda and the sensitivity of GS in Picea abies to vapor pressure deficit (D) was lower than in Pinus taeda. Optimal fertilization increased leaf area index (L) two- and threefold in Pinus taeda and Picea abies, respectively, regardless of whether irrigation was increased. Although it increased L, fertilization did not increase GS in Picea abies unless irrigation was also provided. In Pinus taeda growing on coarse, sandy soils, the doubling of L in response to fertilization reduced GS sharply unless irrigation was also provided. The reduction in GS with fertilization in the absence of irrigation resulted from the production of fine roots with low saturated hydraulic conductivity. When Pinus taeda received both fertilization and irrigation, the increase in L was accompanied by a large increase in GS. In Pinus taeda, a reference GS (defined as GS at D = 1 kPa; GSR) decreased in all treatments with decreasing volumetric soil water content (theta). In Picea abies, theta varied little within a treatment, but overall, GSR declined with theta, reaching lowest values when drought was imposed by the interception of precipitation. Despite the large difference in GS both between Picea abies and Pinus taeda and among treatments, stem growth was related to absorbed radiation, and stem growth response to treatment reflected mostly the changes in L.

  6. Phylogeography of SW Mediterranean firs: different European origins for the North African Abies species.

    Science.gov (United States)

    Sánchez-Robles, Jose M; Balao, Francisco; Terrab, Anass; García-Castaño, Juan L; Ortiz, María A; Vela, Errol; Talavera, Salvador

    2014-10-01

    The current distribution of Western Mediterranean Abies species is a result of complex geodynamic processes and climatic oscillations that occurred in the past. Abies sect. Piceaster offers a good study model to explore how geo-climatic oscillations might have influenced its expansion and diversification on both sides of the W Mediterranean basin. We investigated the genetic variation within and among nine populations from five Abies species by molecular markers with high and low mutation rates and contrasting inheritance (AFLP and cpSSR). Analyses revealed the opening of the Strait of Gibraltar as an effective barrier against gene flow between the Southern Iberian (A. pinsapo) and North African (A. marocana and A. tazaotana) firs. The A. pinsapo populations in Spain and likewise those of the A. marocana - A. tazaotana population complex were not differentiated, and no evidence was found to distinguish A. tazaotana at the species level. Diversification of Abies across North Africa could occur by way of at least two vicariant events from Europe, in the west, giving rise to the A. marocana - A. tazaotana complex, and in the east, giving A. numidica. Secondary contacts among species from Abies sect. Piceaster (A. pinsapo and A. numidica), and with A. alba (Abies sect. Abies) are also indicated. However, there is a closer relationship between the Algerian fir (A. numidica) and the North Mediterranean widespread A. alba, than with the Moroccan firs (A. marocana and A. tazaotana) or the Southern Iberian (A. pinsapo). We also discuss the distribution range of these taxa in its paleogeological and paleoclimatic context, and propose that part of the modern geography of the South-Western Mediterranean firs might be traced back to the Tertiary. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. Identification and Characterization of the Phage Gene sav, Involved in Sensitivity to the Lactococcal Abortive Infection Mechanism AbiV

    DEFF Research Database (Denmark)

    Haaber, Jakob Brandt Borup; Rousseau, G. M.; Hammer, Karin

    2009-01-01

    Lactococcus lactis phage mutants that are insensitive to the recently characterized abortive infection mechanism AbiV were isolated and analyzed in an effort to elucidate factors involved in the sensitivity to AbiV. Whole-genome sequencing of the phage mutants p2.1 and p2.2 revealed mutations...... effect. Conserved, evolutionarily related regions in SaV polypeptides of different phage groups are likely to be responsible for the AbiV-sensitive phenotype and the toxicity....

  8. Green synthesis of silver nanoparticles mediated by Coccinia grandis and Phyllanthus emblica: a comparative comprehension

    Science.gov (United States)

    Nayagam, Vasanth; Gabriel, Melchias; Palanisamy, Kumaravel

    2018-04-01

    Fruit extracts also have the potentiality to synthesize silver nanoparticles, which serve as antimicrobial agent in the biological field. At present, the field of biomedical largely depends on the biosynthesized NPs to fight against the multi-drug-resistant pathogens. The fruit residue of Coccinia grandis and Phyllanthus emblica are employed for synthesizing AgNPs by green method. The NPs are further subjected to UV, FTIR, SEM, and XRD measurements. The ten different pathogens were tested against the AgNPs synthesized. The same were tested for early growth of some seed variety too, so as to check the advantages of AgNPs. The UV spectrum analysis showed 442 nm and 423 nm, respectively, and FTIR peaks for the functional group that is responsible for the conversion of NPs were observed at 1640.02 for N-H bond amines (Coccinia grandis) and at 1637.45 for N-H bond amines (Phyllanthus emblica). The SEM results also illustrated that AgNPs are spherical in shape. The XRD patterns indicate the crystalline nature of the AgNPs formed with both these plants. The antimicrobial assay of AgNPs from Coccinia grandis shows maximum zone of inhibition (14 mm) for Vibrio cholerae whereas the AgNPs from Phyllanthus emblica show maximum inhibition at distinct points, namely for Staphylococcus aureus, Vibrio cholerae, Salmonella typhi, and Proteus mirabilis (12 mm). Seed germination initiated by AgNPs is quiet effective and healthier compared to the water-induced seeds. Hence, biogenic AgNPs have various applications in favour of human society.

  9. Evaluation of Antibacterial Activities of Citrus limon, Citrus reticulata, and Citrus grandis Against Pathogenic Bacteria

    Directory of Open Access Journals (Sweden)

    Sholeh Saeb

    2016-11-01

    Full Text Available Background: Microorganisms resistant to most antibiotics are rapidly spreading, and there is an urgent and continuous need for novel antimicrobial compounds. The genus Citrus belongs to the family Rutaceae has many biologically active secondary metabolites. Objectives: The purpose of this study was to evaluate antimicrobial activity of essential oil and extract of Lemon (Citrus limon, Mandarin (Citrus reticulata and Pummelo (Citrus grandis against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella typhi. Materials and Methods: The fresh Citrus leaves were shade-dried and powdered. Antimicrobial metabolites were extracted from them by 80% methanol for extract and using a Clevenger-type apparatus for essential oil. Eight different concentrations of the each leaf extract and essential oil were prepared. The antimicrobial susceptibility assay of Citrus leaves metabolites were subjected against four bacterial strains by agar disc diffusion and E-test method. Results: In this study, minimum inhibitory concentrations (MIC of different Citrus leaf extracts were determined against all four food-borne pathogens. The C. grandis leaf essential oil had potent antimicrobial activity against all four pathogens, and the C. limon leaf essential oil was effective on Gram-positive bacteria. S. typhi was resistant against two leaves essential oils. Conclusions: The results showed that there was no antimicrobial activity effect in all extracts on tested bacteria. In this study, the antibacterial effect of essential oil of Citrus leaves on four strains of pathogenic microorganisms was confirmed. The C. grandis leaf essential oil had the most powerful antimicrobial properties, suggesting its potential application as natural preservative in foods or an effective medicine against different pathogenic microbes. Key words: Antibacterial activity, E-test, Citr

  10. Antraquinonas e naftoquinonas do caule de um espécime de reflorestamento de Tectona grandi (Verbenaceae)

    OpenAIRE

    Moreira,Rafael Y.O.; Arruda,Mara S.P.; Arruda,Alberto C.; Santos,Lourivaldo S.; Müller,Adolfo H.; Guilhon,Giselle M.S.P.; Santos,Alberdan S.; Terezo,Evaristo

    2006-01-01

    O fracionamento do extrato hexânico do caule de um espécime de reflorestamento de Tectona grandis (Verbenaceae), através de procedimentos fitoquímicos clássicos, levou ao isolamento das naftoquinonas lapachol e desidro-a-lapachona e das antraquinonas tectoquinona e obtusifolina. As estruturas das substâncias foram caracterizadas através da análise de métodos espectrométricos de RMN. Este é o primeiro estudo fitoquímico de um espécime de reflorestamento de Tectona grandis, no Brasil, sendo o o...

  11. PROPRIEDADES TECNOLÓGICAS DA MADEIRA DE Tectona grandis L.f. PROVENIENTE DO VALE DO RIO DOCE, MINAS GERAIS

    OpenAIRE

    MOTTA, J. P.

    2011-01-01

    MOTTA, Javan Pereira. Propriedades tecnológicas da madeira de teca (Tectona grandis L.f.) proveniente do Vale do Rio Doce, Minas Gerais. 2011. Dissertação (Mestrado em Ciências Florestais) Universidade Federal do Espírito Santo, Alegre-ES, Orientador: Prof. Dr. José Tarcísio da Silva Oliveira. Coorientador: Prof. Dr. Juarez Benigno Paes. O objetivo deste trabalho foi determinar as propriedades tecnológicas da madeira de teca (Tectona grandis L.f.) de árvores co...

  12. Kajian Beberapa Sifat Dasar Kayu Ekaliptus (Eucalyptus grandis) Umur 5 Tahun

    OpenAIRE

    Hutagalung, Frans Januari

    2011-01-01

    This study aimed to evaluate the nature of anatomical, physical, and mechanical eucalyptus wood (Eucalyptus grandis), age 5 years vertically and horizontally. The nature of eucalyptus wood anatomy shows that shaped pores solitary and multiple 2-3, eucalyptus stems reddish brown wood, eucalyptus wood looks dull and the direction of the fiber straight to wavy. Eucalyptus wood fiber length average 1103.53 μ, fiber diameter 282.4 μ, lumen diameter 188 μ, and fiber wall thickness 47.2 μ. The physi...

  13. Field response of predatorRhizophagus grandis to prey frass and synthetic attractants.

    Science.gov (United States)

    Wainhouse, D; Beech-Garwood, P A; Howell, R S; Kelly, D; Orozco, M P

    1992-10-01

    A lure based on the proportional composition of monoterpenes inD. micans larval frass and deployed in Theysohn slot traps was highly attractive toR. grandis released in the field. The relative response to frass and lure was consistent over a range of doses, and behavior close to traps baited with either lure or frass appeared to be similar. The monoterpenes, formulated with antioxidant, appear to be stable over several weeks when released from proprietary reservoir and wick "air fresheners." The lure may be of value in monitoring predator populations.

  14. EFFECT OF ARBUSCULAR MYCORRHIZA FUNGI INOCULATION ON TEAK (Tectona grandis Linn. F AT CIKAMPEK, WEST JAVA

    Directory of Open Access Journals (Sweden)

    Ragil S.B. Irianto

    2005-07-01

    Full Text Available The aim of this study was to identify the effect of Arbuscular Mycorhiza Fungi (AMF on the early growth of teak (Tectona grandis Linn. F plantation. Teak seedlings were inoculated with Glomus aggregatum or Mycofer (mixing of four Arbuscular Mycorrhiza Fungi (AMF : G. margarita, G. manihotis, G. etunicatum and Acalospora spinosa at the time of transplantation. At  three months old the seedlings were planted in Cikampek experimental forest. Results showed that application of G. aggregatum or mycofer to teak could accelerate height and diameter growth by up to 61%and4 7%, respectively, after three months in the field.

  15. Annual increments, specific gravity and energy of Eucalyptus grandis by gamma-ray attenuation technique

    International Nuclear Information System (INIS)

    Rezende, M.A.; Guerrini, I.A.; Ferraz, E.S.B.

    1990-01-01

    Specific gravity annual increments in volume, mass and energy of Eucalyptus grandis at thirteen years of age were made taking into account measurements of the calorific value for wood. It was observed that the calorific value for wood decrease slightly, while the specific gravity increase significantly with age. The so-called culmination age for the Annual Volume Increment was determined to be around fourth year of growth while for the Annual Mass and Energy Increment was around the eighty year. These results show that a tree in a particular age may not have a significant growth in volume, yet one is mass and energy. (author)

  16. MANEJO DE RESIDUOS DE Eucalyptus grandis Hill ex Maiden EN SITIOS CONTRASTANTES

    OpenAIRE

    Ana Maria Lupi; Teresa Boca; Maria De Los Angeles Garcia; Diana Diaz; Luciana Ingaramo

    2017-01-01

    En la región NE de Argentina existe poca información sobre el impacto que tienen las diferentes alternativas de manejo de residuos de la cosecha forestal sobre las propiedades edáficas. En este estudio se analizó el efecto a corto plazo de sistemas de manejo de residuos de la cosecha de Eucalyptus grandis Hill ex Maiden, sobre la concentración del carbono orgánico total del suelo (COT) y la fracción particulada (COP), su estado estructural y la conductividad hidráulica saturada. El estu...

  17. Allozyme, chloroplast DNA and RAPD markers for determining genetic relationships between Abies alba and the relic population of Abies nebrodensis.

    Science.gov (United States)

    Vicario, F; Vendramin, G G; Rossi, P; Liò, P; Giannini, R

    1995-06-01

    Allozyme, chloroplast (cpDNA) and random amplified polymorphic DNA (RAPD) markers have been used to estimate genetic and taxonomic relationships among different populations of Abies alba and the relic population of A. nebrodensis. Twelve isozyme gene loci, as well as restriction fragment length polymorphism (RFLP) at cpDNA spacer regions between t-RNA genes were analysed. Moreover, a set of 60 random sequence 10-mer primers were tested. Over all isozyme loci, evident differences in allele frequencies among A. nebrodensis and A. alba populations were found, particularly at 2 loci, phosphoglucose isomerase (Pgi-a) and shikimate dehydrogenase (Skd-a). More than 10% of the total genetic diversity was due to differences among populations. High values of genetic distances among populations were also found. Out of the 60 primers tested, 12 resulted in a polymorphic banding pattern both within and among populations. A total of 84 RAPD fragments were produced by the 12 selected primers. A phenogram of relationships among populations was constructed based on RAPD band sharing: the differentiation of the A. nebrodensis population was evident. The analysis of molecular variance (AMOVA) was used to apportion the variation among individuals within populations and among populations. There was considerable variation within each population: even so, genetic divergence was found among populations. This pattern of genetic variation was very different from that reported for inbred species. Identical cpDNA amplification and restriction patterns were observed among all the individuals sampled from the populations. Taken together, the results of allozyme and RAPDs show a clear differentiation among A. nebrodensis and A. alba populations and provide support for their classification into two different taxonomic groups.

  18. Screening of Methanol Extract and Ethyl Acetate Fraction of Abies webbiana Lindl. for Neuropharmacological Activities.

    Science.gov (United States)

    Parkash, O; Kumar, D; Kumar, S

    2015-01-01

    Despite a long traditional of use of Abies webbiana Lindl. (Talispatra; family-Pinaceae) in the treatment of mental disorders, the plant has not been investigated systematically to validate its traditional claims. Thus, the present investigation was undertaken with an objective to investigate neuropharmacological activities of methanol extract of Abies webbiana aerial parts and its ethyl acetate fraction. Properly identified aerial parts were defatted with petroleum ether and then extracted with methanol in a Soxhlet apparatus. Ethyl acetate fraction was prepared by partitioning methanol extract with ethyl acetate using standard procedure. In acute toxicity study, no mortality was observed in animals after oral administration of 2 g/kg dose of methanol extract. The methanol extract (200 or 400 mg/kg, p.o.) and ethyl acetate fraction (25 or 50 mg/kg, p.o.) were evaluated for antianxiety, anticonvulsant, antidepressant, sedative, antistress and analgesic activities using well established models. The methanol extract and ethyl acetate fraction of Abies webbiana aerial parts exhibited significant antianxiety, anticonvulsant, antidepressant, sedative, antistress and analgesic activities with respect to control. Preliminary phytochemical screening showed presence of flavonoids in bioactive ethyl acetate fraction of Abies webbiana aerial parts. It is finally concluded that flavonoids are the bioactive constituents responsible for most of neuropharmacological activities of Abies webbiana.

  19. Cloning, sequencing and expression of cDNA encoding growth ...

    Indian Academy of Sciences (India)

    Unknown

    cell embryo and the expression was monitored continuously. The expression shown here is in developing embryo and freshly hatched fish. The intensity of green colour indicate the strong expression of EGFP in all the tissues of the embryo/fry. The expression of EGPF indicates the co-expression of catfish GH cDNA and the ...

  20. cDNA cloning, structural analysis, SNP detection and tissue ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 96; Issue 2. cDNA cloning, structural analysis, SNP detection and tissue ... Abstract. Insulin-like growth factor 1 (IGF1) plays an important role in growth, reproduction, foetal development and cell proliferation. The present study was conducted to clone and sequence the ...

  1. Cloning and characterization of cDNA encoding xyloglucan ...

    African Journals Online (AJOL)

    Jane

    2011-08-22

    Aug 22, 2011 ... construction and restructuring of xyloglucan cross-links, thereby controlling the mechanical properties of cell wall. We cloned complete cDNA of an ..... are marked by horizontal lines. The conserved cysteine residues (amino acids 220, 229, 274 and 288 in P. glaucum) are marked by vertical blue arrows.

  2. cDNA, genomic sequence cloning and overexpression of ribosomal ...

    African Journals Online (AJOL)

    RPS16 of eukaryote is a component of the 40S small ribosomal subunit encoded by RPS16 gene and is also a homolog of prokaryotic RPS9. The cDNA and genomic sequence of RPS16 was cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription-polymerase chain ...

  3. cDNA cloning, structural analysis, SNP detection and tissue ...

    Indian Academy of Sciences (India)

    THOMAS NAICY

    [Naicy T., Venkatachalapathy T., Aravindakshan T., Raghavan K. C., Mini M. and Shyama K. 2017 cDNA cloning, structural analysis, SNP detection and tissue expression profile of the IGF1 gene in Malabari and Attappady Black goats of India. J. Genet. 96, xx–xx]. Introduction. Insulin-like growth factor 1 (IGF1), an important ...

  4. Construction of yeast surface-displayed cDNA libraries.

    Science.gov (United States)

    Bidlingmaier, Scott; Liu, Bin

    2011-01-01

    Using yeast display, heterologous protein fragments can be efficiently displayed at high copy levels on the Saccharomyces cerevisiae cell wall. Yeast display can be used to screen large expressed protein libraries for proteins or protein fragments with specific binding properties. Recently, yeast surface-displayed cDNA libraries have been constructed and used to identify proteins that bind to various target molecules such as peptides, small molecules, and antibodies. Because yeast protein expression pathways are similar to those found in mammalian cells, human protein fragments displayed on the yeast cell wall are likely to be properly folded and functional. Coupled with fluorescence-activated cell sorting, yeast surface-displayed cDNA libraries potentially allow the selection of protein fragments or domains with affinity for any soluble molecule that can be fluorescently detected. In this report, we describe protocols for the construction and validation of yeast surface-displayed cDNA libraries using preexisting yeast two-hybrid cDNA libraries as a starting point.

  5. A BIOINFORMATIC STRATEGY TO RAPIDLY CHARACTERIZE CDNA LIBRARIES

    Science.gov (United States)

    A Bioinformatic Strategy to Rapidly Characterize cDNA LibrariesG. Charles Ostermeier1, David J. Dix2 and Stephen A. Krawetz1.1Departments of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, & Institute for Scientific Computing, Wayne State Univer...

  6. Contenido de nutrientes en las raices finas y el mantillo de rodales de Eucalyptus grandis de diferente edad en la Mesopotomia Argentina [Fine roots and litter nutrient content of Eucalyptus grandis stands presenting different ages in Mesopotomia Argentina

    Science.gov (United States)

    C. Perez; J. Frangi; J.F. Goya; A. Luy; M. Arturi; NO-VALUE

    2013-01-01

    Entre Ríos province is an important center of Eucalyptus spp. plantations in Argentina. It was hypothesized that fine root biomass and litter mass increased with age increasing in plantations. Five, seven and seventeen year old stands of Eucalyptus grandis were sampled. All of them were first rotation stands. We estimated the mass of litter and fine roots (

  7. Primeiro registro de Oligonychus yothersi (McGregor (Acari: Tetranychidae em Eucalyptus grandis Hill ex Maiden no Brasil First record of Oligonychus yothersi (McGregor (Acari: Tetranychidae on Eucalyptus grandis Hill ex Maiden in Brazil

    Directory of Open Access Journals (Sweden)

    Fabrício Fagundes Pereira

    2005-08-01

    Full Text Available Relata-se a infestação de um ácaro-vermelho em mudas clonais de Eucalyptus grandis Hill ex Maiden, mantidas em casa de vegetação no município de Martinho Campos, Minas Gerais. O ácaro foi observado na parte superior das folhas que exibiam sinais de sucção de seiva e bronzeamento. Essas injúrias causaram desenvolvimento anormal e morte de plantas. O ácaro foi identificado como Oligonychus yothersi (McGregor (Acari: Tetranychidae, e isso representa o primeiro registro dessa espécie em mudas clonais de E. grandis no Brasil.An infestation of the red spider mite was reported in clone seedlings of Eucalyptus grandis Hill ex Maiden under greenhouse conditions, in the municipality of Martinho Campos, Minas Gerais State. The spider mite was found on the leaf upper faces with signs of sap suction and bronzing. Such injuries caused abnormal development and plant death. The spider mite was identified as Oligonychus yothersi (McGregor (Acari: Tetranychidae. This is the first record of O. yothersi on E. grandis seedlings in Brazil.

  8. Exploiting Measurement Uncertainty Estimation in Evaluation of GOES-R ABI Image Navigation Accuracy Using Image Registration Techniques

    Science.gov (United States)

    Haas, Evan; DeLuccia, Frank

    2016-01-01

    In evaluating GOES-R Advanced Baseline Imager (ABI) image navigation quality, upsampled sub-images of ABI images are translated against downsampled Landsat 8 images of localized, high contrast earth scenes to determine the translations in the East-West and North-South directions that provide maximum correlation. The native Landsat resolution is much finer than that of ABI, and Landsat navigation accuracy is much better than ABI required navigation accuracy and expected performance. Therefore, Landsat images are considered to provide ground truth for comparison with ABI images, and the translations of ABI sub-images that produce maximum correlation with Landsat localized images are interpreted as ABI navigation errors. The measured local navigation errors from registration of numerous sub-images with the Landsat images are averaged to provide a statistically reliable measurement of the overall navigation error of the ABI image. The dispersion of the local navigation errors is also of great interest, since ABI navigation requirements are specified as bounds on the 99.73rd percentile of the magnitudes of per pixel navigation errors. However, the measurement uncertainty inherent in the use of image registration techniques tends to broaden the dispersion in measured local navigation errors, masking the true navigation performance of the ABI system. We have devised a novel and simple method for estimating the magnitude of the measurement uncertainty in registration error for any pair of images of the same earth scene. We use these measurement uncertainty estimates to filter out the higher quality measurements of local navigation error for inclusion in statistics. In so doing, we substantially reduce the dispersion in measured local navigation errors, thereby better approximating the true navigation performance of the ABI system.

  9. Estimate of the density of Eucalyptus grandis W. Hill ex Maiden using near infrared spectroscopy

    Directory of Open Access Journals (Sweden)

    Silviana Rosso

    2013-12-01

    Full Text Available This study aimed to analyze use of near infrared spectroscopy (NIRS to estimate wood density of Eucalyptus grandis. For that, 66 27-year-old trees were logged and central planks were removed from each log. Test pieces 2.5 x 2.5 x 5.0 cm in size were removed from the base of each plank, in the pith-bark direction, and subjected to determination of bulk and basic density at 12% moisture (dry basis, followed by spectral readings in the radial, tangential and transverse directions using a Bruker Tensor 37 infrared spectrophotometer. The calibration to estimate wood density was developed based on the matrix of spectra obtained from the radial face, containing 216 samples. The partial least squares regression to estimate bulk wood density of Eucalyptus grandis provided a coefficient of determination of validation of 0.74 and a ratio performance deviation of 2.29. Statistics relating to the predictive models had adequate magnitudes for estimating wood density from unknown samples, indicating that the above technique has potential for use in replacement of conventional testing.

  10. Insights into temperature modulation of the Eucalyptus globulus and Eucalyptus grandis antioxidant and lignification subproteomes.

    Science.gov (United States)

    de Santana Costa, Marília Gabriela; Mazzafera, Paulo; Balbuena, Tiago Santana

    2017-05-01

    Eucalyptus grandis and Eucalyptus globulus are among the most widely cultivated trees, differing in lignin composition and plantation areas, as E. grandis is mostly cultivated in tropical regions while E. globulus is preferred in temperate areas. As temperature is a key modulator in plant metabolism, a large-scale proteome analysis was carried out to investigate changes in the antioxidant system and the lignification metabolism in plantlets grown at different temperatures. Our strategy allowed the identification of 3111 stem proteins. A total of 103 antioxidant proteins were detected in the stems of both species. Hierarchical clustering revealed that alterations in the antioxidant proteins are more prominent when Eucalyptus seedlings were exposed to high temperature and that the superoxide isoforms coded by the gene Eucgr.B03930 are the most abundant antioxidant enzymes induced by thermal stimulus. Regarding the lignin biosynthesis, our proteomics approach resulted in the identification of 13 of the 17 core proteins involved in this metabolism, corroborating with gene predictions and the proposed lignin toolbox. Quantitative analyses revealed significant differences in 8 protein isoforms, including the ferulate 5-hydroxylase isoform F5H1, a key enzyme in catalyzing the synthesis of sinapyl alcohol, and the cinnamyl alcohol dehydrogenase isoform CAD2, the last enzyme in monolignol biosynthesis. Data are available via ProteomeXchange with identifier PXD005743. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Use of glass transition temperature for stabilization of board's cracks of Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Fred W. Calonego

    2010-09-01

    Full Text Available The Eucalyptus grandis logs temperatures were determined and correlated with the board's cracks during steaming. Thermocouples were inserted in the logs center, registering their temperatures during steaming at 90"C. The logs were sawed and the board's cracks measured. It was concluded that: (1 the logistic S-shaped curve explains the logs temperature variation; (2 the logs with diameter of 20 to As temperaturas em toras de Eucalyptus grandis, durante a vaporização, foram determinadas e correlacionadas com as rachaduras das tábuas. Nos centros das toras foram inseridos termopares e registradas suas temperaturas durante a vaporização à 90"C. As toras foram desdobradas e as rachaduras das tábuas mensuradas. Concluiu-se que: (1 o modelo estatístico sigmoidal logístico explica a variação da temperatura nas toras; (2 as toras com 20 a <25, 25 a <30 e 30 a <35 cm de diâmetro apresentaram, respectivamente, 84,2"C, 73,1"C e 45,8"C ao final da vaporização; e (3 as rachaduras foramsignificativamente menores nas toras que atingiram a temperatura de transição vítrea.

  12. Determination of chemical elements in Eucalyptus grandis, manured with Ballad's, by neutrons activation analysis

    International Nuclear Information System (INIS)

    Mateus, Natalina de Fatima; Madi Filho, Tufic

    2007-01-01

    The biosolid is a mud resulting from the biological treatment of wasted liquids. It is considered as a profitable alternative and important to minimize the environmental impact generated by the sewage thrown in to sanitary lands, in forest cultures like the Eucalyptus grandis. The objective of this work was to detect which chemical elements are present in Eucalyptus grandis samples, fertilized with different quantities of biosolid. The eucalyptuses of Estacao Experimental de Ciencias Florestais of Itatinga were planted in March of 1998 and collected with five years old. The used biosolid was produced by Station of Treatment of Sewer of Barueri - SP, classified as kind B. For the determination of the presence and quantity of chemical elements in the eucalyptus samples, an analysis technique by neutronic activation (NAA) was used followed by gamma rays spectroscopy. The samples were irradiated in the Nuclear Reactor IEA-R1 of IPEN-SP, followed by the measure of induced gamma rays activity, using a Detector HPGe. The presence, mainly of Br, Mn, Na and K, was detected in all analyzed samples. (author)

  13. Determination of heavy metals in Eucalyptus grandis, manured with biosolid, by neutrons activation analysis

    International Nuclear Information System (INIS)

    Mateus, Natalina de Fatima; Yamashita, Marcelo Takeshi; Madi Filho, Tufic

    2007-01-01

    The biosolid is a mud resulting from the biological treatment of wasted liquids. It is considered as a profitable alternative and important to minimize the environmental impact generated by the sewage thrown in the sanitary lands. The utilization of biosolid in forest cultures, as the Eucalyptus grandis, is of great economic and scientific interest, because it promotes not only the use of sewage residues, but also a fertilization prices reduction. The objective of this work was to detect the presence of heavy metals in Eucalyptus grandis sample fertilized with different quantities of biosolid. For the experiment, we used the plantation of Estacao Experimental de Ciencias Florestais of Itatinga, linked to ESALQ of Universidade de Sao Paulo - USP. The eucalyptus were planted in March of 1998 and collect with five years old. The used biosolid was produced by ETE of Barueri - SP, classified as kind B. The samples were prepared in Universidade Estadual Paulista of Itapeva. For the determination of heavy metals presence in eucalyptus samples, an analysis technique by neutronic activation (NAA) was used followed by gamma rays spectroscopy. The samples were irradiated in the Nuclear Reactor IEA-R1 of IPEN-SP, followed by the measure of induced gamma rays activity, using a Detector HPGe. The presence, mainly of Br, Mn, Na and K, was detected in all analyzed samples. (author)

  14. Antimicrobial activity of protease inhibitor from leaves of Coccinia grandis (L.) Voigt.

    Science.gov (United States)

    Satheesh, L Shilpa; Murugan, K

    2011-05-01

    Antimicrobial activity of protease inhibitor isolated from Coccinia grandis (L.) Voigt. has been reported. A 14.3 kDa protease inhibitor (PI) was isolated and purified to homogeneity by ammonium sulfate precipitation (20-85% saturation), sephadex G-75, DEAE sepharose column and trypsin-sepharose affinity chromatography from the leaves of C. grandis. The purity was checked by reverse phase high performance liquid chromatography. PI exhibited marked growth inhibitory effects on colon cell lines in a dose-dependent manner. PI was thermostable and showed antimicrobial activity without hemolytic activity. PI strongly inhibited pathogenic microbial strains, including Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris, Eschershia coli, Bacillus subtilis and pathogenic fungus Candida albicans, Mucor indicus, Penicillium notatum, Aspergillus flavus and Cryptococcus neoformans. Examination by bright field microscopy showed inhibition of mycelial growth and sporulation. Morphologically, PI treated fungus showed a significant shrinkage of hyphal tips. Reduced PI completely lost its activity indicating that disulfide bridge is essential for its protease inhibitory and antifungal activity. Results reported in this study suggested that PI may be an excellent candidate for development of novel oral or other anti-infective agents.

  15. Light intensity and type of container on producing Cassia grandis L. f. seedlings

    Directory of Open Access Journals (Sweden)

    Caio C. P. Leal

    2015-10-01

    Full Text Available ABSTRACTThis study aimed to determine the effects of the luminosity and type of container on producing Cassia grandis seedlings. Thus, in a substrate composed by topsoil + wood powder (1:1 the seedlings were grown into plastic tubets containing 0.3 dm3 of such substrate, or into plastic pots containing 1.0 dm3 of the same substrate, and subjected to 50 and 25% shading or full sunlight. The assessments were performed every two weeks by measuring plant height and stem diameter, during eight weeks period. At the end of this period, the leaf area, dry mass of shoots and roots, the ratio between height of plant/diameter of stem, and the Dickson quality index were also assessed. The experiment was carried out in a completely randomized design with treatments arranged in a 3 x 2 factorial [3 luminosity levels (50, 25% shading and full sunlight x 2 types of containers (plastic tubets and clay pots] with four replicates of 10 seedlings each. Means were compared by Tukey test at 0.05 probability level and adjustments for the non-linear regression polynomial curves for the assessed characteristics were computed in function of time. Shadings of 25 and 50% were the best alternatives to luminosity; and the tubets were the most suitable containers for production of Cassia grandis seedlings.

  16. The GOES-R ABI Wild Fire Automated Biomass Burning Algorithm

    Science.gov (United States)

    Hoffman, J.; Schmidt, C. C.; Prins, E. M.; Brunner, J. C.

    2011-12-01

    The global Wild Fire Automated Biomass Burning Algorithm (WF_ABBA) at the Cooperative Institute for Meteorological Satellite Studies (CIMSS) provides fire detection and characterization using data from a global constellation of geostationary satellites, currently including GOES, MTSAT, and Meteosat. CIMSS continues to enhance the legacy of the WF_ABBA by adapting the algorithm to utilize the advanced spatial, spectral, and temporal capabilities of GOES-R ABI. A wide range of simulated ABI data cases have been generated and processed with the GOES-R fire detection and characterization algorithm. Simulated cases included MODIS derived projections as well as model derived simulations that span a variety of satellite zenith angles and ecosystems. The GOES-R ABI fire product development focuses on active fire detection and sub-pixel characterization, including fire radiative power (FRP) and instantaneous fire size and temperature. With the algorithm delivered to the system contractor, the focus has moved to developing innovative new validation techniques.

  17. Species variation in susceptibility to the fungus Neonectria neomacrospora in the genus Abies

    DEFF Research Database (Denmark)

    Nielsen, Ulrik Braüner; Xu, Jing; Nielsen, Knud Nor

    2017-01-01

    ; significant differences were seen between taxa, that is, species, and between some species and their subspecies. The Greek fir, Abies cephalonica, was the only species without damage. An inoculation experiment on detached twigs with mycelium plugs from a N. neomacrospora culture showed that all species could......The fungus Neonectria neomacrospora has recently caused an epidemic outbreak in conifer species within the genus Abies in Denmark and Norway. Christmas tree producers in Europe and North America rely, to a large extent, on Abies species. The damage caused by N. neomacrospora, including dead shoot...... be infected. The damage observed in the inoculation experiment could explain 30% of the variation in the field observations based on species mean values. The epidemic outbreak and the high number of species susceptible to this fungus indicate that N. neomacrospora requires attention in the cultivation...

  18. Differential regulation of macropinocytosis by Abi1/Hssh3bp1 isoforms.

    Directory of Open Access Journals (Sweden)

    Patrycja M Dubielecka

    2010-05-01

    Full Text Available Macropinocytosis, which is a constitutive cellular process of fluid and macromolecule uptake, is regulated by actin cytoskeleton rearrangements near the plasma membrane. Activation of Rac1, which is proposed to act upstream of the actin polymerization regulatory Wave 2 complex, has been found to correlate with enhanced macropinocytosis. One of the components of the Wave 2 complex is Abi1. Multiple, alternatively spliced isoforms of Abi1 are expressed in mammalian cells, but the functional significance of the various isoforms is unknown.Here, using flow cytometric assay analysis for Alexa Fluor 647, we demonstrate that Abi1 isoforms 2 and 3 differentially regulate macropinocytosis. LNCaP cells expressing isoform 3 had increased macropinocytic uptake that correlated with enhanced cell spreading and higher Rac1 activation in comparison to cells expressing isoform 2. Isoform 2 expressing cells had decreased macropinocytic uptake, but demonstrated greater sensitivity to Rac1 activation. Moreover, more isoform 2 was localized within the cytoplasm in comparison to isoform 3, which was more associated with the plasma membrane. Activated Rac1 was found to specifically bind to a site in exon 10 of isoform 2 in vitro. Because of alternative mRNA splicing, exon 10 is absent from isoform 3, precluding similar binding of activated Rac1. Both isoforms, however, bound to inactive Rac1 through the same non-exon 10 site. Thus, Abi1 isoform 3-containing Wave 2 complex exhibited a differential binding to activated vs. inactive Rac1, whereas isoform 2-containing Wave 2 complex bound activated or inactive Rac1 comparably.Based on these observations, we postulate that Abi1 isoforms differentially regulate macropinocytosis as a consequence of their different relative affinities for activated Rac1 in Wave 2 complex. These findings also raise the possibility that isoform-specific roles occur in other Abi1 functions.

  19. Evaluating variations of physiology-based hyperspectral features along a soil water gradient in Eucalyptus grandis plantation

    CSIR Research Space (South Africa)

    Cho, Moses A

    2010-04-01

    Full Text Available Remote sensing is viewed as a cost-effective alternative to intensive field surveys in assessing site factors that affect growth of Eucalyptus grandis over broad areas. The objective of this study was to assess the utility of hyperspectral remote...

  20. Evaluating variations of physiology-based hyperspectral features along a soil water gradient in a Eucalyptus grandis plantation

    NARCIS (Netherlands)

    Cho, M.A.; Aardt, van J.; Main, R.; Majeke, B.

    2010-01-01

    Remote sensing is viewed as a cost-effective alternative to intensive field surveys in assessing site factors that affect growth of Eucalyptus grandis over broad areas. The objective of this study was to assess the utility of hyperspectral remote sensing to discriminate between site qualities in E.

  1. Interspecific hybridization and inbreeding effect in seed from a Eucalyptus grandis x E. urophylla clonal orchard in Brazil

    Directory of Open Access Journals (Sweden)

    Campinhos Eduardo N.

    1998-01-01

    Full Text Available We used allozyme markers to estimate the amount of natural hybridization between Eucalyptus grandis and E. urophylla in a 7.4-hectare commercial hybrid-seed orchard planted in Espírito Santo, Brazil. This orchard was planted in 1982 using a honeycomb design, with each hexagonal plot containing one E. grandis tree surrounded by six E. urophylla trees. There were 267 replicated hexagonal plots in the orchard. Seeds were harvested from the E. grandis clone only. The multilocus outcrossing rate estimated for the E. grandis clone averaged 70.2%, ranging from 33.0 to 99.0% among individual trees. Contaminant pollination, inferred from progeny genotypes containing alleles not present in the seven parental clones, accounted for 14.4% of the hybrid seed. Contaminant pollen was attributed to neighboring eucalyptus stands isolated from the orchard by a 400-m wide belt of native forest. Inbred and hybrid progenies were identified by their allozyme genotypes and transplanted to the field. Field growth of inbred progeny was 30% lower than that of hybrid plants at two and three years of age.

  2. Bleaching of kraft pulps produced from green liquor pre-hydrolyzed South African Eucalyptus grandis wood chips

    CSIR Research Space (South Africa)

    Andrew, JE

    2014-01-01

    Full Text Available The effect of hemicellulose pre-extraction of South African Eucalyptus grandis wood chips using green liquor, on subsequent kraft pulping and bleaching processes was studied. This was done in the context of a biorefinery mill producing both ethanol...

  3. Variation in water-use efficiency and delta-C-13 levels in eucalyptus-grandis clones

    CSIR Research Space (South Africa)

    Olbrich, BW

    1993-10-01

    Full Text Available This study aimed to determine whether the delta-C-13 levels in the foliage and twigs of four Eucalyptus grandis clones were related to their water use efficiency (WUE). This relationship has previously been demonstrated in a number of herbaceous...

  4. Macromycetes in communities of Abies alba on its range border in central Poland

    Directory of Open Access Journals (Sweden)

    Jarosłaaw Szkodzik

    2014-08-01

    Full Text Available The paper presents results of mycocoenological examinations conducted in four nature reserves with Abies alba in Central Poland (Lubiaszów, Kruszewiec, Łaznów and Gałków in 1997-1999. A list of 288 fungi accompanying silver fir communities close to the northern limit of the occurrence of the species, including 38 species identified in associations with Abies alba in Poland for the first time, is given. Statistical analysis has shown that a relationship exists between macromycete diversity, tree stand structure and plant communities such as Tilio-Carpinetum, Luzulo pilosaee-Fagetum, Pino-Quercetum abietetosum.

  5. [Free sugars, cyclitols and organic acids in the cambial sap of Pinus sylvestris L., Picea Abies Karst. and Abies alba Mill].

    Science.gov (United States)

    Kretz, A

    1973-03-01

    Samples of cambial sap from each of the three coniferous species Pinus sylvestris L., Picea Abies Karst. and Abies alba Mill. were taken at the time the trees were coming into bud and analysed for low molecular weight carbohydrates, cyclitols and organic acids. They all contained the same free sugars and cyclitols, but in markedly different proportions. Quantitative analyses were carried out for glucose, fructose, sucrose, raffinose, myo-inositol, D-inositol, pinitol, sequoyitol and coniferin.The three main components of the organic acid fractions-quinic acid, shikimic acid and malic acid-were determined quantitatively. The amount of quinic acid greatly exceeded the amount of all the other acids in all three species. (14)C-labelled quinic acid applied to the cut ends of Pinus sylvestris needles was transported to the twig. There was no conversion of quinic acid to shikimic acid over short periods of time.

  6. Nutrients in foliage and wet deposition of nitrate, ammonium and sulfate in washing tree top in Abies religiosa forests

    Science.gov (United States)

    E.R Peña-Mendoza; A. Gómez-Guerrero; Mark Fenn; P. Hernández de la Rosa; D. Alvarado Rosales

    2016-01-01

    The nutritional content and tree top in the forests are evaluated of Abies religiosa, San Miguel Tlaixpan (SMT) and Rio Frio (RF), State of Mexico. The work had two parts. In the first, the nutritional content was evaluated in new foliage (N, P, K, Ca and Mg) in Abies religiosa trees, in periods of spring, summer and winter, in...

  7. Effect of abscisic acid on stomatal opening in isolated epidermal strips of abi mutants of Arabidopsis thaliana

    NARCIS (Netherlands)

    Roelfsema, MRG; Prins, HBA

    1995-01-01

    Abscisic acid-insensitive mutants of Arabidopsis thaliana L. var. Landsberg erecta were selected for their decreased sensitivity to ABA during germination. Two of these mutants, abi-1 and abi-2, display a wilty phenotype as adult plants, indicating disturbed water relations. Experiments were

  8. Brotación in vitro de yemas de teca (Tectona grandis L. f. Sprouting buds in vitro teak (Tectona grandis L. f.

    Directory of Open Access Journals (Sweden)

    Fabiana Rojas Parajeles

    2012-11-01

    Full Text Available La teca (Tectona grandis L.f. es una especie maderable exótica introducida a Costa Rica para la siembra de plantaciones comercial por la alta demanda de su madera, su rápido crecimiento y su alta calidad. Inicialmente se utilizó la semilla como único material de siembra y la pobre calidad de muchas de las plantaciones impulsó el inicio de programas de mejora genética. La propagación clonal tomó mucha importancia en estos programas y el cultivo in vitro se convirtió en una herramienta valiosa para la propagación masiva de los árboles élites. Por lo anterior, este trabajo se enfocó en evaluar el efecto de varias concentraciones de dos reguladores de crecimiento, bencilaminopurina (BA y ácido indolacético (AIB, solos y en combinación, en la brotación de yemas dormantes y formación de callos de teca. Tanto el análisis estadístico como la observación visual mostraron que el tratamiento que consistió de 0,005 mg/l de AIA fue el mejor para incrementar la brotación de las yemas y para disminuir la formación de callo.Teak (Tectona grandis L. f. is an exotic timber species introduced to Costa Rica for commercial plantations due to the high demand for its wood, rapid growth and high-quality. Initially the seed was use as the only planting material, but the poor quality of many of the plantations resulted in the initiation of genetic improvement programs. The introduction of clonal propagation in these programs and the establishment of in vitro culture techniques became import tools for mass propagation of the selected elite trees. On the foregoing, this work focused on assessing the effect of several concentrations of two growth regulators, benzylaminopurine (BA and indole acetic acid (AIB, alone and in combination, in the budding of dormantes buds and callus formation of teak. Both the statistical analysis and the visual observation showed that the treatment consisted of 0.005 mg/l of IAA was the best to increase the budding of the

  9. Organogênese de explante foliar de clones de Eucalyptus grandis x E. urophylla Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones

    Directory of Open Access Journals (Sweden)

    Elisa Cristina Soares de Carvalho Alves

    2004-05-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos dos reguladores de crescimento TDZ [1-fenil-3-(1,2,3-tia-diazol-5-iluréia], BAP (6-benzilaminopurina e ANA (ácido naftalenoacético no desempenho da propagação in vitro por organogênese de explante foliar de três clones híbridos de Eucalyptus grandis x Eucalyptus urophylla. Houve resposta diferenciada dos clones quanto a intensidade, textura e coloração dos calos, em razão dos tratamentos com os reguladores de crescimento. Os melhores resultados de calejamento dos três genótipos foram observados nos tratamentos com a combinação dos reguladores de crescimento TDZ (0,5 mg L-1 e ANA (0,1 mg L-1, obtendo-se 100% de calejamento no explante foliar. Os piores resultados de calejamento foram observados nos tratamentos com a combinação dos reguladores de crescimento BAP (0,1 mg L-1 e ANA (0,1 mg L-1. Em relação à regeneração, a melhor resposta foi obtida com 1,0 mg L-1 BAP em que 8% dos calos formados a partir de explantes foliares regeneraram gemas, com número médio destas formadas por calo igual a 4,2.The aim of this work was to evaluate the effects of growth regulators TDZ [1-phenil-3-(1,2,3-thiadiazol-5-yl urea], BAP (6-benzilaminopurine e NAA (Naphthalene acetic acid on the in vitro propagation by organogenesis from foliar explants of Eucalyptus grandis x E. urophylla. Depending on the clone used, there were singular responses to growth regulators treatment regarding callusing intensity, texture and color. The best results of the three genotypes used were observed with the TDZ (0.5 mg L-1 and NAA (0.1 mg L-1 treatment, where 100% of the foliar explants presented callus. The worst results were observed with the BAP (0.1 mg L-1 and NAA (0.1 mg L-1 treatment. Subsequently, considering the regeneration process, the best response was achieved with 1.0 mg L-1 BAP, in which 8% of the calli regenerated buds, with an average of 4.2 buds per explant.

  10. Arabinogalactan-protein epitope Gal4 is differentially regulated and localized in cell lines of hybrid fir (Abies alba x Abies cephalonica) with different embryogenic and regeneration potential.

    Science.gov (United States)

    Samaj, Jozef; Salaj, Terézia; Matúsová, Radoslava; Salaj, Ján; Takác, Tomás; Samajová, Ol'ga; Volkmann, Dieter

    2008-02-01

    Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that highly embryogenic cell line AC78 of hybrid fir (Abies alba x Abies cephalonica) differs from very low-embryogenic cell line AC77 in the abundance, subcellular localization and deposition of subset of secreted AGPs. A specific AGP epitope containing Gal residues and reacting to Gal4 antibody is secreted and deposited into ECMSN, which covers the surface of the embryogenic cells showing high embryogenic and regeneration capacity in the cell line AC78. On the other hand, this Gal4 AGP epitope was not secreted and/or found on the surface of meristematic cells showing low embryogenic and regeneration capacity in the cell line AC77, as well as on the surface of non-embryogenic suspensor cells and callus cells in both cell lines AC77 and AC78. As a positive control, we have used another AGP epitope LM2 (containing glucuronic acid) showing no significant differences in these two Abies hybrid lines. This study defines specific AGPs containing beta-(1-->6)-galactotetraosyl group as a first molecular component of ECMSN covering embryogenic cells in gymnosperms.

  11. Revisión taxonómica de Alytes grandis Brunner (Amphibia, Anura

    Directory of Open Access Journals (Sweden)

    Sanchiz, B.

    1986-12-01

    Full Text Available The holotype and only existing remain referred to Alytes grandis Brunner, 1957, presumably a discoglossid frog from the German Pleistocene, is examined and compared with other living and fossil Palaearctic anurans. The analysis rejects its validity as an independent extinct species, as The material is clearly within the known variability of living Rana temporaria Linnaeus, 1758, of which it should be considered merely a sinonym.

    Se examina el holotipo y único resto atribuido de Alytes grandis Brunner, 1957, un supuesto discoglósido del Pleistoceno alemán, comparándose con otros anuros actuales y fósiles del Paleártico. El análisis permite desechar su pertenencia a una especie extinta independiente, siendo en cambio atribuible con toda claridad a la viviente Rana temporaria Linnaeus, 1758, de la que debe considerarse sinónimo.
    Zum systematischen Status von Alytes grandis Brunner, 1957 (Amphibia, Anura. Der bislang einzig bekannte und von Brunner (1957 beschriebene Rest zu Alytes grandis aus pleistozänen Ablagerungen der Breitenberghöhle bei Gössweinstein (Fränk, Alb wird beschrieben und aufgrund ausführlicher Vergleichsuntersuchungen der rezenten Art Rana temporaria Linnaeus, 1758, zugeordnet, Alytes grandis Brunner, 1957, ist somit als synonyrn zu Rana temporaria zu betrachten.

  12. Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae and Cross-Amplification in Related Mediterranean Species

    Directory of Open Access Journals (Sweden)

    Laura Navarro-Sampedro

    2012-11-01

    Full Text Available Twelve nuclear microsatellite primers (nSSR were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454 data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.

  13. Nuclear microsatellite primers for the endangered relict fir, Abies pinsapo (Pinaceae) and cross-amplification in related Mediterranean Species.

    Science.gov (United States)

    Sánchez-Robles, Jose M; Balao, Francisco; García-Castaño, Juan L; Terrab, Anass; Navarro-Sampedro, Laura; Talavera, Salvador

    2012-11-05

    Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.

  14. Molecular cloning of growth hormone encoding cDNA of Indian ...

    Indian Academy of Sciences (India)

    A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in ...

  15. cDNA cloning and characterization of a mannose-binding lectin from ...

    Indian Academy of Sciences (India)

    Using RNA extracted from Zingiber officinale rhizomes and primers designed according to the conservative regions of monocot mannose-binding lectins, the full-length cDNA of Z. officinale agglutinin (ZOA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zoa was 746 bp and contained a ...

  16. Isolation and characterization of a Coffea canephora ERF-like c-DNA

    African Journals Online (AJOL)

    A cDNA corresponding to an ERF gene has been isolated from a Coffea canephora fruit cDNA library. The cDNA was 1,317 nucleotides long and has an open reading frame of 987 bp. The predicted polypeptide showed a great similitude with equivalent proteins from others plant species. The binding domain shows 98.3% ...

  17. Molecular cloning of growth hormone encoding cDNA of Indian

    Indian Academy of Sciences (India)

    A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in ...

  18. cDNA cloning and characterization of a mannose-binding lectin

    Indian Academy of Sciences (India)

    Using RNA extracted from Zingiber officinale rhizomes and primers designed according to the conservative regions of monocot mannose-binding lectins, the full-length cDNA of Z. officinale agglutinin (ZOA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zoa was 746 bp and contained a ...

  19. cDNA library information - Dicty_cDB | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available (C) 5) sexually fusion-competent KAX3 cells (Gamete phase) (F) cDNA library construction method How to const...dir) 2) Full-length cDNA libraries (oligocapped method)(fl) 3) Gamete-specific subtraction library (sub) cDNA library construction

  20. [CDNA cloning of human leptin and its expression].

    Science.gov (United States)

    Jia, Zhen-Yu; Fu, Xiao-Min; Jin, Ai-Hua; Cao, Jiang

    2003-07-01

    To clone cDNA of human leptin gene and obtain leptin protein for future study on leptin binding proteins. The cDNA of human leptin with 6 x his-tag was cloned by over-hang extension PCR protocol using human genomic DNA as template, and subcloned into in vitro expression vector pIVEX2.3MCS, and the fusion protein was expressed in vitro by Rapid Translation System (RTS) (RTS500 cycle primer Kit and RTS500 ProteoMaster of Roche company). The apparent molecular weight(19.46 kD) and the immuno-specificity of the fusion protein were confirmed by SDS-PAGE and Western blot, and the expressed fusion protein stayed mainly in the supernatant of the reaction mixture in soluble form. This work provides us solid basis for further study on new leptin-associated proteins.

  1. Construction of cDNA library of Pyrocystis lunula (Pyrophyta)

    Science.gov (United States)

    Sui, Zhenghong; Kowallik, Klaus V.

    2004-10-01

    Complementary DNA library of a dinoflagellate Pyrocystis lunula was constructed for the purpose of expression sequence tags analysis. The RNA isolated from this alga was about 20µgg-1 net cells, and the band intensity ratio of 28S/18 S in electrophoresis pattern was nearly 1 to 1. Different cDNA/vector molar ratios were exploited in the ligating reaction to be optimized. The clones produced by cDNA/vector molar ratio of 3.75 to 1 were desirable, most of whose inserts were longer than 300 bp. The recombinants insert length of the unfractionation cDNA library was largely shorter than 500 bp. However, in the fractionation library made from high molecule weight cDNA parts, over seventy percent of the recombinants contained inserts longer than 1 kb, some of which were even longer than 3 kb. Operating concerns were discussed at the end.

  2. Migration and dispersal of Anthonomus grandis (Coleoptera: Curculionidae in South America

    Directory of Open Access Journals (Sweden)

    Teodoro STADLER

    2007-01-01

    Full Text Available El presente estudio sobre la dispersión de Anthonomus grandis Boheman, el picudo del algodonero, en Argentina, Brasil, Paraguay y Bolivia, explora las características ecológicas y fisiológicas que han permitido a este insecto dispersarse y establecerse exitosamente en América del Sur. La plasticidad fenotípica de A. grandis se caracteriza por un tiempo de desarrollo flexible, ciclo de vida multivoltino con generaciones superpuestas, la capacidad de alimentarse con polen de diversas familias botánicas así como de otras fuentes de alimento y por su habilidad para migrar y dispersarse con la ayuda del viento. Todo esto hace de esta especie una plaga clave para el cultivo del algodón. Los cultivos de cítricos en Misiones, Argentina, son posibles sitios para la hibernación de esta especie. En esta región fueron capturadas grandes cantidades de individuos prediapausantes, provenientes de algodonales en post-cosecha en Paraguay, atraídos probablemente por compuestos volátiles de cítricos cultivados en la zona. La quiescencia facultativa que atraviesan los adultos ante condiciones adversas, conlleva a un retraso en el desarrollo que se relaciona con las condiciones desfavorables. Esto sugiere que la hibernación en A. grandis puede ser definida como «oligopausa», una forma intermedia de diapausa. Desde su introducción en Brasil en 1983 y hasta el 2006, el picudo se ha dispersado en dirección sudoeste hacia Argentina, a una velocidad promedio de 61 km año-1. Sin embargo, le ha insumido aproximadamente diez años cruzar 250 km, desde Paraguay hacia el centro de la zona algodonera de Argentina. Este progreso más lento se debe probablemente a las acciones llevadas a cabo en el marco del programa de erradicación del picudo del algodonero, por parte del gobierno de Argentina. La llegada del picudo al área central de cultivo de algodón en la Argentina, así como a otras áreas de cultivo en Paraguay y Argentina, confirma el hecho de que

  3. Biomass of clone of Eucalyptus grandis x urophylla for producing briquettes; Biomassa de clone de Eucalyptus grandis x Eucalyptus urophylla para producao de briquetes

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Eder Aparecido; Oguri, Guilherme [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas], e-mail: os_garcias@fca.unesp.br; Lancas, Kleber Pereira [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Engenharia Rural; Guerra, Saulo Philipe Sebastiao [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Gestao e Tecnologia Agroindustrial

    2011-07-01

    The aim of this work was conducted to address forest biomass energy for briquette producing. In an area of dystrophic soil, seedlings of clones of Eucalyptus grandis x E. urophylla were planted in 2008, considering factors spacing and fertilization. The first dosage of fertilizer was 70 g/plant of NPK 6-30-6 and total coverage of 110 g/plant of NPK 20-0-20 with B and Zn. The spacing was 2.8x0.5 m, 2.8x1.0 m, 2.8x1.5 m, 2.8x2.0 m and 2.8x2.5 m. At 18 months, tree samples were collected to evaluate the basic density of wood (BDW), dry biomass of stem, branches and leaves. An assessment of the economic viability of each treatment was based on the sale of briquettes. BDW spacing of 2.8x1.0 m was 0.464 kg/m{sup 3}. The largest biomass of the stem occurred in 2.8x0.5 m spacing, with dosage 3, but economically unviable. The dry biomass of branches was only affected by dosage, reaching 17.68 t/ha in the third dose. Only fertilization was significant for leaf biomass. The highest income in the spacing was 2.8 x1.5 m with dosage 2. (author)

  4. Construction of cDNA libraries in vaccinia virus.

    Science.gov (United States)

    Smith, Ernest S; Shi, Shuying; Zauderer, Maurice

    2004-01-01

    Poxvirus expression vectors have gained widespread use for expression of foreign proteins and as delivery vehicles for vaccine antigens. We have developed a novel method using the poxvirus as a library vector for functional selection of specific cDNA. Poxviruses have several unique and useful properties as a library vector. Most importantly, because poxviruses are packaged into fully infectious particles in the cell cytoplasm, specific recombinants can be readily recovered even from a very small number of selected cells. Moreover, in contrast to libraries constructed in retrovirus or plasmid-based vectors, recombinant vaccinia virus can be efficiently recovered even from cells that have been induced to undergo apoptosis or cessation of cell growth. In the past, the major obstacle in this application to poxviruses has been the low frequency with which recombinants can be generated. The most commonly used method to construct recombinant poxvirus is homologous recombination. The frequency of recombinants derived in this manner is of the order of 0.1%, sufficient to recover a recombinant of a purified DNA clone in a transfer plasmid, but far too low to permit construction of a representative cDNA library. We have developed a method that generates nearly 100% recombinant vaccinia viruses at good titer. We have termed this method trimolecular recombination. cDNA libraries of as many as 107 or more independent viral recombinants can be constructed by trimolecular recombination. For the first time, large, diverse, and representative cDNA libraries can be screened in a vaccinia virus-based expression vector.

  5. cDNA cloning, structural analysis, SNP detection and tissue ...

    Indian Academy of Sciences (India)

    THOMAS NAICY

    E-mail: naicy@kvasu.ac.in. and conception rate ... transformed into DH5α strain of Escherichia coli and the clones harbouring ... Primer pairs for caprine IGF1 and GAPDH were designed using Primer3 software (table 1). RTq-PCR was conducted in a 25 μL reaction volume containing 50 ng of cDNA and 2× Max- ima SYBR ...

  6. CDNA encoding a polypeptide including a hevein sequence

    Science.gov (United States)

    Raikhel, Natasha V.; Broekaert, Willem F.; Chua, Nam-Hai; Kush, Anil

    1995-03-21

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

  7. cDNA library generation from ribonucleoprotein particles.

    Science.gov (United States)

    Rederstorff, Mathieu; Hüttenhofer, Alexander

    2011-02-01

    Most, if not all, known noncoding RNAs (ncRNAs) are associated with RNA binding proteins, thus forming ribonucleoprotein particles or RNPs. Here we describe a protocol for the generation of a specialized cDNA library from RNPs, thereby increasing the proportion of functional ncRNA species in the library. To that end, cellular extracts are fractionated on 10-30% glycerol gradients. Subsequently, RNP-derived ncRNAs are isolated and 3'-tailed by cytidine triphosphate and poly(A) polymerase; this is followed by 5' adapter ligation by T4 RNA ligase. Reverse transcription of ncRNAs into cDNAs is carried out with an oligo-d(G) anchor primer. The generated cDNA libraries are subsequently submitted to high-throughput sequencing. This RNP selection procedure increases the probability of the presence of biologically relevant ncRNA species in the library compared with libraries generation methods that use size-selected, protein-devoid ncRNAs. The protocol enables the generation of deep-sequencing-compatible cDNA libraries that code for functional ncRNAs within 1 week.

  8. cDNA encoding a polypeptide including a hevein sequence

    Energy Technology Data Exchange (ETDEWEB)

    Raikhel, N.V.; Broekaert, W.F.; Chua, N.H.; Kush, A.

    2000-07-04

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74--79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

  9. In Vitro antioxidant activity of extracts from the leaves of Abies ...

    African Journals Online (AJOL)

    Traditionally, the leaves of Abies pindrow Royle are employed as an ayurvedic remedy for fever, hypoglycaemic, respiratory and inflammatory conditions. In this study, dichloromethane, methanol and acetone extracts of A. pindrow leaves were analysed for their phytochemical content and in vitro antioxidant activities.

  10. Abiotic Factors and Yushania Influences on Abies Forest Composition in Taiwan

    Directory of Open Access Journals (Sweden)

    Cheng-Tao Lin

    2014-09-01

    Full Text Available Abies kawakamii forests are generally distributed above 3,000 m in Taiwanese high mountains. The community data used in our analysis were derived from the database of the National Vegetation Diversity Inventory and Mapping Project of Taiwan (NVDIMP, and environmental data were obtained from the WorldClim and NVDIMP databases. We used non-metric multidimensional scaling (NMDS to identify vegetation composition of Abies communities and the structural equation models (SEMs were used to examine the complex relationships between environmental factors and vegetation composition. The results of ordination showed the most important factors determining species composition of Abies forests involved habitat rockiness, heat load index, warmth index and summer and winter. SEM results approved the warmth index and winter precipitation were the main drivers determining the latent variable—climate, which significantly affect the overstory composition of Abies communities. The relative frequency of Yushania niitakayamensis also had a minor effect on the overstory. However, the relative frequency of Yushania had strong and significant effects on the understory flora and it was also strongly correlated with rock ratio and winter precipitation. Moreover, the overstory displayed a negative but insignificant coefficient on the understory composition, and this might be attributed to the fragile and high heterogeneous habitat in Taiwanese high mountain areas.

  11. Abies religiosa habitat prediction in climatic change scenarios and implications for monarch butterfly conservation in Mexico

    Science.gov (United States)

    Cuauhtemoc Saenz-Romero; Gerald E. Rehfeldt; Pierre Duval; Roberto A. Lindig-Cisneros

    2012-01-01

    Abies religiosa (HBK) Schl. & Cham. (oyamel fir) is distributed in conifer-dominated mountain forests at high altitudes along the Trans-Mexican Volcanic Belt. This fir is the preferred host for overwintering monarch butterfly (Danaus plexippus) migratory populations which habitually congregate within a few stands now located inside a Monarch Butterfly Biosphere...

  12. Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

    Czech Academy of Sciences Publication Activity Database

    Bříza, Jindřich; Pavingerová, Daniela; Vlasák, Josef; Niedermeierová, Hana

    2013-01-01

    Roč. 60, č. 3 (2013), s. 395-400 ISSN 0001-527X R&D Projects: GA MZe QH71290; GA ČR(CZ) GAP502/11/1471 Institutional support: RVO:60077344 Keywords : Cry3A gene modification * Picea abies * Agrobacterium tumefaciens Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.389, year: 2013

  13. Pakistan vajab abi - kas maailma tõesti ei huvita? / Urmas Jaagant

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2010-01-01

    Pakistan saab igal aastal suurt rahvusvahelist abi. Mitmed riigid on üleujutustes Pakistani toetanud nüüdki kümnete miljonite dollaritega, kuid riikide tahe annetada on erinev, sest mitte iga abidollarit ei suunata Pakistanis sinna, kus seda tegelikult vajatakse

  14. Allozyme variation and possible phylogenetic implications in Abies cephalonica Loudon and some related eastern Mediterranean firs

    Science.gov (United States)

    B. Fady; M. T. Conkle

    1993-01-01

    A total of 22 loci were assayed in several populations of Abies cephalonica, A. borisii regis, A. bornmuelleriana and A. alba using horizontal starch gel electrophoresis. Within and between-population diversity were analyzed as well as between-species diversity. Mean expected heterozygosity was...

  15. Exogenous putrescine affects endogenous polyamine levels and the development of Picea abies somatic embryos

    Czech Academy of Sciences Publication Activity Database

    Vondráková, Zuzana; Eliášová, Kateřina; Vágner, Martin; Martincová, Olga; Cvikrová, Milena

    2015-01-01

    Roč. 75, č. 2 (2015), s. 405-414 ISSN 0167-6903 R&D Projects: GA MŠk(CZ) LD13050 Institutional support: RVO:61389030 Keywords : Exogenous putrescine * Somatic embryogenesis * Picea abies Subject RIV: ED - Physiology Impact factor: 2.333, year: 2015

  16. [Genetic control of Silver fir isozymes (Abies alba Mill.) of the Ukrainian Carpathian Mountains].

    Science.gov (United States)

    Korshikov, I I; Morozova, N N; Pirko, Ia V

    2003-01-01

    Genetic control of GOT, GDH, DIA, MDH, ME, SOD, FDH, ADH, ACP, LAP enzymes has been studied in the seed megagametophytes of Silver fir (Abies alba Mill.) from four natural populations of the Ukrainian Carpathian mountains. The distinct electrophoretic division has been obtained for the 21 loci products. The analysis of allele segregation in the heterozygous trees confirms monogenic inheritance of the revealed variants.

  17. Plant communities of silver fir (Abies alba) forests in southeastern Bohemia

    Czech Academy of Sciences Publication Activity Database

    Boublík, Karel; Zelený, D.

    2007-01-01

    Roč. 27, - (2007), s. 73-90 ISSN 0722-494X R&D Projects: GA ČR(CZ) GA206/05/0020 Institutional research plan: CEZ:AV0Z60050516 Keywords : Abies alba * coniferous forests * Czech Republic Subject RIV: EF - Botanics

  18. Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies.

    Science.gov (United States)

    Siljak-Yakovlev, S.; Cerbah, M.; Coulaud, J.; Stoian, V.; Brown, S. C.; Zoldos, V.; Jelenic, S.; Papes, D.

    2002-02-01

    Two closely related spruces, Picea abies and Picea omorika, a Balkan paleoendemic species, often share habitats, yet never hybridize in nature. The present study adresses their characteristics such as nuclear DNA content, base composition, heterochromatin and rDNA pattern. The genome size of P. abies was 10% larger than that of P. omorika when assessed by flow cytometry, respectively 2C=37.2 pg and 33.8 pg; although when estimated as total chromosome length it was virtually the same. The heterochromatin Chromomycin-A (CMA)/ DAPI fluorochrome banding patterns of both P. abies and P. omorikaare given here for the first time. Simultaneous FISH (fluorescent in situ hybridization) using 18S-26S and 5S rDNA probes revealed 16 18S rDNA sites in P. omorika, 12 18S rDNA sites in P. abies, and a single 5S rDNA locus in both species. The genomes have about 41% GC. The number and position of CMA/DAPI bands and rDNA loci provide good chromosome markers to clarify the karyotypes of the two species.

  19. Isolation and characterization of microsatellite markers in Fraser fir (Abies fraseri)

    Science.gov (United States)

    S.A. Josserand; K.M. Potter; G. Johnson; J.A. Bowen; J. Frampton; C.D. Nelson

    2006-01-01

    We describe the isolation and characterization of 14 microsatellite loci from Fraser fir (Abies fraseri). These markers originated from cloned inserts enriched for DNA sequences containing tandem di- and tri-nucleotide repeats. In total, 36 clones were selected, sequenced and evaluated. Polymerase chain reaction (PCR) primers for 14 of these...

  20. Polyamines in embryogenic cultures of Norway spruce (Picea abies) and red spruce (Picea rubens)

    Science.gov (United States)

    Rakesh Minocha; Haarald Kvaalen; Subhash C. Minocha; Stephanie Long

    1993-01-01

    Embryogenic cultures of red spruce (Picea rubens Sarg.) and Norway spruce (Picea abies (L.) Karst.) were initiated from dissected mature zygotic embryos. The tissues were grown on either proliferation medium or maturation medium. On proliferation medium, the embryogenic tissue continued to produce early stage somatic embryos (...

  1. Complex bud architecture and cell-specific chemical patterns enable supercooling of Picea abies bud primordial

    Science.gov (United States)

    Bud primordia of Picea abies, despite a frozen shoot, stay ice free down to -50 °C by a mechanism termed supercooling whose biophysical and biochemical requirements are poorly understood. Bud architecture was assessed by 3D-reconstruction, supercooling and freezing patterns by infrared video thermog...

  2. Fractionation of organic substances from the South African Eucalyptus grandis biomass by a combination of hot water and mild alkaline treatments

    CSIR Research Space (South Africa)

    Johakimu, Jonas K

    2015-09-01

    Full Text Available An alternative way of fractionating lignocellulose biomass into its individual components, hemicelluloses, lignin and cellulose, was investigated. South African Eucalyptus grandis wood chips were fractionated using a combination of hot water...

  3. Wood properties of Eucalyptus grandis and Pinus caribaea estimated by colorimetry

    Directory of Open Access Journals (Sweden)

    Patrícia Gomes Ribeiro Amorim

    2013-09-01

    Full Text Available The use of non-destructive technologies has been very effective in characterization and evaluation of wood quality. This study aimed to characterize the technologically wood of Pinus caribaea and Eucalyptus grandis by Colorimetry. The study was conducted at the University of Brasilia (UNB in Physics and Engineering Laboratory the Laboratory of Forest Products/LPF - Brazilian Forest Service (SFB. Species were subjected to standard tests of basic density, shrinkage and bending. For the colorimetric determination of the parameters it was used a spectrophotometer according to the methodology adopted by Camargos (1999. The radial surface of the wood is most suitable for the collecting of the colorimetric parameters and prediction of properties. Property best estimated by this technique was the density, followed by modulus of elasticity (MOE and modulus of rupture (MOR.

  4. EFFECT OF THE STEAMING ON THE DRYING OF Eucalyptus grandis BOARDS

    Directory of Open Access Journals (Sweden)

    Raphael Nogueira Rezende

    2015-03-01

    Full Text Available The objective of this work was to evaluate the effect of the steaming on the drying rate and drying quality of Eucalyptus grandis boards. For this purpose, wood from an experimental plantation of the Federal University of Lavras, Minas Gerais State, Brazil, with 24 years of age was used. Trees were felled and sectioned in logs and the logs were sawn by a tangential system. Half of the boards volume were steamed during 3 hours at 90ºC of temperature and 100% of relative humidity after the heating of the drying process. The other half was not steamed (control. The boards were dried in the dry-kiln and the resulting defects from the drying process and drying rate were determined. The results indicated that the steaming was effective in increase of the drying rate in 15% and decrease of the drying defects of 20 to 52%.

  5. A Multivariate Study on Genetic Variation in Teak (Tectona grandis (L.))

    DEFF Research Database (Denmark)

    Kjær, Erik Dahl; Siegismund, Hans Redlef; Suangtho, V.

    1996-01-01

    , but there were also substantial variation within ecological-geographical defined regions. The differentiation between provenances from Laos were found to be less than the variation within Thailand, West India and Indonesia. A much less pronounced differentiation between populations was found in allozyme markers......Genetic differentiation between populations of teak (Tectona grandis (L.)) was examined in 9 quantitative characters and 10 allozyme loci. Large differences between populations were revealed in the quantitative traits. Regional patterns were revealed by multivariate analysis of the data....... The fixation index (FST) was only 4%, and no clear geographical pattern was found in the allozyme data. However, some differences were found in the level of diversity. An assignment procedure based on allozyme markers did not prove efficient in differentiating between populations, but this was probably...

  6. Genetic resources of teak (Tectona grandis Linn. f.)—strong genetic structure among natural populations

    DEFF Research Database (Denmark)

    Hansen, Ole Kim; Changtragoon, Suchitra; Ponoy, Bundit

    2015-01-01

    Twenty-nine provenances of teak (Tectona grandis Linn. f.) representing the full natural distribution range of the species were genotyped with microsatellite DNA markers to analyse genetic diversity and population genetic structure. Provenances originating from the semi-moist east coast of India...... of genetic diversity supports the hypothesis that teak has its centre of origin in India, from where it spread eastwards. The analysis of molecular variance (AMOVA) gave an overall highly significant F st value of 0.227—population pairwise F st values were in the range 0.01–0.48. Applying the G......″st differentiation parameter, the estimated overall differentiation was 0.632, implying a strong genetic structure among populations. A neighbour-joining (NJ) tree, using the pairwise population matrix of G″st values as input, contained three distinct groups: (1) the eight provenances from Thailand and Laos, (2...

  7. Chemical Composition of Essential Oil from the Peel of Chinese Torreya grandis Fort

    Directory of Open Access Journals (Sweden)

    Tao Feng

    2011-01-01

    Full Text Available The composition of the peel essential oil of Torreya grandis fort obtained by cold pressing and steam distillation was determined by GC and GC/MS. 62 constituents accounting for 99.6% of the total pressed oil were identified while 59 compounds accounting for 99.4% of the steam distilled oil were identified. Limonene (35.6–37.1%, α-pinene (20.1–24.1%, and δ-carene (3.3–3.9 were the major constituents. Others include γ-carene (3.8-3.9%, germacrene D (2.5–2.9%, and β-farnesene (2.7-2.8%.

  8. TECHNOLOGICAL CHARACTERISTICS OF THE WOOD OF Eucalyptus grandis W.Hill ex Maiden AND Eucalyptus cloeziana F. Muell, AS A SUPPLY FOR THE FURNITURE INDUSTRY

    OpenAIRE

    Joaquim Carlos Gonçalez; Lílian de Cássia S. Breda; João Francisco M. Barros; Denízia Gonçalves Macedo; Gerard Janin; Alexandre Florian da Costa; Ailton Teixeira do Vale

    2010-01-01

    This work was carried out at Universidade de Brasília at the Wood Products of Forestry Laboratory (IBAMA, Brasília, DF, Brazil). Two species of eucalypt wood (Eucalyptus grandis W.Hill ex Maiden and Eucalyptus cloeziana) were studied to supply the furniture making industry. The wood of Eucalyptus grandis W.Hill ex Maiden shows physical properties (density and retractibility) and mechanical properties (static bending and hardness) very suitable for the industry of wood furniture. Such characte...

  9. Antioxidant capacity and larvicidal activity of essential oil and extracts from Lippia grandis

    Directory of Open Access Journals (Sweden)

    Evelyn Ivana T. Damasceno

    2011-02-01

    Full Text Available The leaves and thin branches of Lippia grandis Schauer, Verbenaceae, are used for flavoring of food in the Brazilian Amazon, as substitute for oregano. In this study the constituents of the essential oil were identified and the antioxidant capacity and larvicidal activity of the oil and methanol extract and its sub-fractions were evaluated. A sensory evaluation was determined in view of absence of toxicity. The oil showed a yield of 2.1% and its main constituents were thymol (45.8%, p-cymene (14.3%, γ-terpinene (10.5%, carvacrol (9.9% and thymol methyl ether (4.8%, totalizing 85%. The DPPH radical scavenging activity showed values for the EC50 between 9.0 and 130.5 µg mL-1 and the TEAC/ABTS values varied from 131.1 to 336.0 mg TE/g, indicating significant antioxidant activity for the plant. The total phenolic content ranged from 223.0 to 761.4 mg GAE/g, contributing to the antioxidant activity observed. The crude extracts inhibited the bleaching of β-carotene and the oil showed the greatest inhibition (42.5%. The oil (LgO, 7.6±2.4 µg mL-1 showed strong larvicidal activity against the brine shrimp bioassay. The sensory evaluation was highly satisfactory in comparison to oregano. The results are very promising for the use of L. grandis in seasoning and antioxidant products.

  10. Temperature effects on wood anatomy, wood density, photosynthesis and biomass partitioning of Eucalyptus grandis seedlings.

    Science.gov (United States)

    Thomas, D S; Montagu, K D; Conroy, J P

    2007-02-01

    Wood density, a gross measure of wood mass relative to wood volume, is important in our understanding of stem volume growth, carbon sequestration and leaf water supply. Disproportionate changes in the ratio of wood mass to volume may occur at the level of the whole stem or the individual cell. In general, there is a positive relationship between temperature and wood density of eucalypts, although this relationship has broken down in recent years with wood density decreasing as global temperatures have risen. To determine the anatomical causes of the effects of temperature on wood density, Eucalyptus grandis W. Hill ex Maiden seedlings were grown in controlled-environment cabinets at constant temperatures from 10 to 35 degrees C. The 20% increase in wood density of E. grandis seedlings grown at the higher temperatures was variously related to a 40% reduction in lumen area of xylem vessels, a 10% reduction in the lumen area of fiber cells and a 10% increase in fiber cell wall thickness. The changes in cell wall characteristics could be considered analogous to changes in carbon supply. Lumen area of fiber cells declined because of reduced fiber cell expansion and increased fiber cell wall thickening. Fiber cell wall thickness was positively related to canopy CO2 assimilation rate (Ac), which increased 26-fold because of a 24-fold increase in leaf area and a doubling in leaf CO2 assimilation rate from minima at 10 and 35 degrees C to maxima at 25 and 30 degrees C. Increased Ac increased seedling volume, biomass and wood density; but increased wood density was also related to a shift in partitioning of seedling biomass from roots to stems as temperature increased.

  11. Evaluation of Larvicidal Activity of Essential Oil from Leaves of Coccinia grandis against Three Mosquito Species

    Directory of Open Access Journals (Sweden)

    Shahid Iqbal Mohammed

    2017-06-01

    Full Text Available Background: To study the chemical constituents and larvicidal activity of essential oil extracted from the leaves of Coccinia grandis against three mosquito species.Methods: Essential oil was extracted by hydro distillation using clevenger apparatus and was analyzed for chemical constituents by gas chromatography-mass spectrophotometry (GC-MS. Larvicidal activity was recorded after 12 and 24h of post-exposure against three mosquito species, Anopheles stephensi, Aedes aegypti and Culex quinquefascia­tus. Dead larvae were identified when they failed to move after probing with a needle in the siphon or cervical re­gion. The LC50 and LC90 values for three mosquito larvae were calculated by Probit analysis.Results: The GC-MS analysis revealed that essential oil contains 23 different constituents. Out of these 23 constitu­ents, major constituents identified were n-tetracosane (39.18%, n-eicosane (30.04%, tetratriacotane (2.97%, 7-oc­tadecanal (2.81%, and tricosane (2.31%. Essential oil from leaves of Coccinia grandis exhibited significant larvi­cidal activity against An. stephensi with LC50 and LC90 values 39.41ppm and 123.24ppm, respectively. This was fol­lowed by Ae. aegypti and Cx. quinquefasciatus with LC50 and LC90 values of 48.20ppm, 131.84ppm and 52.80ppm, 135.48ppm, respectively after 24h of exposure.Conclusion: The results could be useful in developing a cost effective, ecofriendly, region specific and practical strategy for the control of mosquito vectors.

  12. [cDNA library construction from panicle meristem of finger millet].

    Science.gov (United States)

    Radchuk, V; Pirko, Ia V; Isaenkov, S V; Emets, A I; Blium, Ia B

    2014-01-01

    The protocol for production of full-size cDNA using SuperScript Full-Length cDNA Library Construction Kit II (Invitrogen) was tested and high quality cDNA library from meristematic tissue of finger millet panicle (Eleusine coracana (L.) Gaertn) was created. The titer of obtained cDNA library comprised 3.01 x 10(5) CFU/ml in avarage. In average the length of cDNA insertion consisted about 1070 base pairs, the effectivity of cDNA fragment insertions--99.5%. The selective sequencing of cDNA clones from created library was performed. The sequences of cDNA clones were identified with usage of BLAST-search. The results of cDNA library analysis and selective sequencing represents prove good functionality and full length character of inserted cDNA clones. Obtained cDNA library from meristematic tissue of finger millet panicle represents good and valuable source for isolation and identification of key genes regulating metabolism and meristematic development and for mining of new molecular markers to conduct out high quality genetic investigations and molecular breeding as well.

  13. Is Prey Specificity Constrained by Geography? Semiochemically Mediated Oviposition in Rhizophagus grandis (Coleoptera: Monotomidae) with Its Specific Prey, Dendroctonus micans (Coleoptera: Curculionidae: Scolytinae), and with Exotic Dendroctonus species.

    Science.gov (United States)

    Dohet, Loïc; Grégoire, Jean-Claude

    2017-08-01

    Examples of totally specific predators are rare, and the mechanisms underlying this specificity are often poorly understood. In Eurasia, the Monotomid beetle Rhizophagus grandis is found only in the galleries of its prey, the bark beetle Dendroctonus micans. The specificity of R. grandis relies on kairomones which female predators use to adjust their oviposition to the number of prey larvae available in a gallery. Yet these chemical signals are still largely unknown. The North American D. punctatus and D. valens, which are not sympatric with R. grandis but have a similar ecology as D. micans, could also elicit predator oviposition, which would suggest that specificity in this predator-prey system is constrained by geography. In order to further identify these determinants of specificity, we used artificial oviposition boxes to compare the oviposition level of R. grandis in the presence of larvae of each of the three prey species. We jointly used sequential dynamic headspace extractions and gas chromatography coupled with mass spectrometry to investigate oviposition stimuli associated with each prey species and potential oviposition inhibitors emitted by the predator. We further assessed potential stimuli with the analysis of emissions from D. micans larvae reared alone. Overall, we identified and quantified 67 compounds, mostly terpenes. Several robust candidate stimulants or inhibitors of R. grandis' oviposition were identified. The three prey species elicited similar oviposition levels in R. grandis, which suggests that this predator could form new associations outside of its native range.

  14. [Construction and identification of the expression library of album pollen allergens cDNA].

    Science.gov (United States)

    Zhang, Jie; Sun, Xiu-zhen; Yan, Hong; Zhang, Ni; Feng, Xiang-li

    2011-05-01

    To construct and identify the express library of album pollen allergens cDNA. Total RNA were extracted from the album pollen with TRIzol reagent and the mRNA was isolate for the amplify followed. A double stranded cDNA (ds cDNA) was synthesized using primers containing Xho I and Poly(dT) sequence by ZAP Express®cDNA synthesis kit. The ds cDNA was modified and purified by gel chromatography, and then the cDNA fragment with the length of more than 400 bp containing sticky ends was obtained. The cDNA fragment was ligated with Uni-ZAP XR vector and subsequently treated with in vitro packaging using phage by ZAP-cDNA express GigapackIII Gold cloning kit. The express library of album pollen cDNA was constructed by in vitro packaging. The recombination rate and the lengths of fragments inserted of the cDNA library were detected by polymerase chain reaction. The titer and the recombination rate of cDNA expression library constructed were 9.7×10(5) and 100%, respectively. The capacity of the library was 4.85 Pfu. The average length of cDNA fragments inserted was about 1.0 kb. Based on the capacity of cDNA expression library constructed and the length of cDNA insertion fragments, the cDNA expression library constructed is qualified to screening target cDNA clone, laying the foundation for preparation of gene recombinant allergen pollen vaccine.

  15. Estimation of strength and stiffness of Eucalyptus grandis wood by ultrasound waves Estimativa da resistência e rigidez da madeira de Eucalyptus grandis por meio de onda ultrassonora

    Directory of Open Access Journals (Sweden)

    Magnos Alan Vivian

    2012-03-01

    Full Text Available

    This study aimed to evaluate the use of dynamic elastic constant obtained in non-destructive testing, as a parameter to estimate the mechanical properties of stiffness and resistance of Eucalyptus grandis. For this we used an emitter of ultrasonic waves, which had its sensitivity evaluated by comparison with the values obtained in the destructive test of static bending. The results show that the dynamic elastic constant can be used as non-destructive inference of the stiffness and resistance of Eucalyptus grandis, though with reservations, because the adjustments observed were relatively low, with R²adj. 0.25 and 0.39, respectively.

     

    doi: 10.4336/2012.pfb.32.69.109

    O presente estudo objetivou avaliar o uso da constante elástica dinâmica obtida no ensaio não destrutivo, como parâmetro para estimativa das propriedades mecânicas de rigidez e resistência da madeira de Eucalyptus grandis. Para isto, utilizou-se um emissor de ondas ultrassônicas, o qual teve sua sensibilidade avaliada através da comparação com os valores obtidos no ensaio destrutivo de flexão estática. Os resultados evidenciam que a constante elástica dinâmica pode ser utilizada para inferência não destrutiva da rigidez e resistência da madeira de Eucalyptus grandis, entretanto com ressalvas, pois os ajustes observados foram relativamente baixos, com R²aj. de 0,25 e 0,39, respectivamente.

     

    doi: 10.4336/2012.pfb.32.69.109

  16. Hydraulic traits are associated with the distribution range of two closely related Mediterranean firs, Abies alba Mill. and Abies pinsapo Boiss.

    Science.gov (United States)

    Peguero-Pina, José Javier; Sancho-Knapik, Domingo; Cochard, Hervé; Barredo, Gonzalo; Villarroya, Dido; Gil-Pelegrín, Eustaquio

    2011-10-01

    Abies alba and Abies pinsapo are two closely related fir species that occur in the Iberian Peninsula under very different environmental conditions. Abies alba proliferates in the humid European mountains, including the Spanish Pyrenees. In contrast, A. pinsapo is a relict species that occurs in some restricted areas of the Mediterranean mountain ranges in Spain and Morocco, which experience intense summer drought periods. To cope with the high atmospheric evaporative demand during summer, A. pinsapo may either have a high resistance to xylem cavitation or develop a very efficient conducting system to reduce the soil-to-leaf water potential gradient. To investigate such hypotheses, we measured (i) the xylem vulnerability to cavitation for different populations, and (ii) several anatomical and hydraulic parameters indicating xylem sufficiency for -supplying water to the shoot in two contrasting populations of both species. Our results show that the resistance to cavitation was not different between species or populations. However, hydraulic conductivity (K(h)), specific hydraulic conductivity (K(s)), leaf-specific conductivity (LSC) and whole-shoot hydraulic conductance (K(shoot)) were higher in A. pinsapo, indicating a higher efficiency of water transport, which should contribute to maintaining its xylem tension below the threshold for rapidly increasing cavitation. The higher K(s) in A. pinsapo was largely a result of its wider tracheids, suggesting that this species may be much more vulnerable to freeze-thaw-induced cavitation than A. alba. This is consistent with the absence of A. pinsapo in northern mountain ranges with cooler winters. These physiological differences could partly explain the niche segregation and the geographical separation of these two firs.

  17. Eficiência das minicepas e microcepas na produção de propágulos de clones de Eucalyptus grandis Efficiency of ministumps and microstumps of Eucalyptus grandis clones for minicutting and microcutting production

    Directory of Open Access Journals (Sweden)

    Miranda Titon

    2003-10-01

    Full Text Available No presente estudo objetivou-se avaliar a eficiência dos jardins clonais nas técnicas de microestaquia e miniestaquia de quatro clones de Eucalyptus grandis, quanto a sobrevivência, vigor e capacidade produtiva das microcepas e minicepas em coletas sucessivas de microestacas e miniestacas. As microcepas utilizadas foram oriundas de mudas rejuvenescidas por micropropagação, mediante subcultivos in vitro, e as minicepas, de miniestacas oriundas de plantas propagadas pelo método da estaquia convencional (macroestaquia. As minicepas e as microcepas apresentaram desempenho similar, tendo sido observado, para ambas, elevada taxa de sobrevivência, vigor e capacidade produtiva de material para propagação vegetativa.The objective of this work was to evaluate the efficiency of clonal gardens in the microcutting and minicutting techniques of four Eucalyptus grandis clones regarding the survival, vigor and productive capacity of the microstumps and ministumps in successive collections of microcuttings and minicuttings. The microstumps were obtained from rejuvenated tissue explants through subculture in vitro, and the ministumps by minicuttings derived from conventional rooted cuttings. The ministumps and microstumps of E. grandis exhibited similar performance, i.e., high survival rate, vigor and productive capacity of vegetative propagation material.

  18. Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments

    NARCIS (Netherlands)

    Hakvoort, T. B.; Spijkers, J. A.; Vermeulen, J. L.; Lamers, W. H.

    1996-01-01

    We have developed a fast and general method to obtain an enriched, full-length cDNA expression library with subtractively enriched cDNA fragments. The procedure relies on RecA-mediated triple-helix formation of single-stranded cDNA fragments with a double-stranded cDNA plasmid library. The complexes

  19. Toward a cDNA map of the human genome

    Energy Technology Data Exchange (ETDEWEB)

    Korenberg, J.R.; Chen, X.N. [Cedars-Sinai Research Institute, Los Angeles, CA (United States); Adams, M.D.; Venter, J.C. [Institute for Genomic Research, Gaithersburg, MD (United States)

    1995-09-20

    Advances in the Human Genome Project are shaping the strategies for identifying the 50,000-100,000 human genes. High-resolution genetic maps of the human genome combined with sequencing herald an era of rapid regional definition of disease genes. However, only once their chromosomes band location is known will the systematic partial sequencing of thousands of random cDNA clones provide the reagents for the rapid assessment of the genes responsible for the inherited disorders. We now present an approach to the rapid determination of map position and therefore to the creation of a transcribed map of the human genome. Sensitive fluorescence in situ hybridization has been combined with high-resolution chromosome banding and random cDNA sequencing to 41 cDNAs with an average insert size of < 2 kb to single human chromosome bands. The results provide 15 new genes, with database and functional information, as candidates for human disease. These include the large extracellular single-related kinase (HUMERK), the ERK activator kinase (PRKMK1), a new member of the RAS oncogene family, protein phosphotase 2 regulatory subunit B alpha isoform (PPP2R2A), and a novel human gene with very high homology to a plant membrane transport family. Further, an analysis of expressed genes associated with pseudogenes showed that by using these techniques, it is possible to detect accurately the transcribed locus within a multigene or processed pseudogene family in most cases. These findings suggest that direct cDNA mapping using fluorescence in situ hybridization provides an accurate and rapid approach to the definition of a transcribed map of the human genome. This low-cost, high-resolution (205 Mb) mapping greatly enhances the speed with which these genes can be subsequently assigned to contigs. This assignment provides a necessary first step in understanding the relationship of the genes to both acquired and inherited human diseases. 16 refs., 1 fig., 3 tabs.

  20. Micobiota associada à madeira serrada de Eucalyptus grandis Hill ex Maiden durante a secagem ao ar livre

    Directory of Open Access Journals (Sweden)

    João Basílio Mesquita

    2006-01-01

    Full Text Available This work’s main objective was to follow the air-drying of Eucalyptus grandis lumber, carried out in Lavras, MG, and to identify the mycobiota associated with the lumber at various phases of drying. The lumbers were obtained from eight 3.0 meter long logs from 27 year-old trees, planted in an experimental area of UFLA. Results showed that the air-drying of Eucalyptus grandis, started in January, demanded 158 days to achieve moisture content around 12.5 %. The moisture content-time curve was represented by a logarithmic equation with coefficient of determination equal to 98.3 %. The highest occurrence of fungi that cause surface mold and stain was observed at the beginning of the drying. The highest frequencies of fungi were verified at the beginning of the drying for Penicillium spp and Pestalotiopsis sp. The fungus Lentinus lepideus occurred with higher frequency at the end of the drying.

  1. Antraquinonas e naftoquinonas do caule de um espécime de reflorestamento de Tectona grandi (Verbenaceae

    Directory of Open Access Journals (Sweden)

    Rafael Y.O. Moreira

    Full Text Available O fracionamento do extrato hexânico do caule de um espécime de reflorestamento de Tectona grandis (Verbenaceae, através de procedimentos fitoquímicos clássicos, levou ao isolamento das naftoquinonas lapachol e desidro-a-lapachona e das antraquinonas tectoquinona e obtusifolina. As estruturas das substâncias foram caracterizadas através da análise de métodos espectrométricos de RMN. Este é o primeiro estudo fitoquímico de um espécime de reflorestamento de Tectona grandis, no Brasil, sendo o objetivo principal deste trabalho a comprovação da presença de tectoquinona em espécimes cultivados.

  2. Oxidative stability of cnicken thigh meat after treatment of abies alba essential oil

    Directory of Open Access Journals (Sweden)

    Adriana Pavelková

    2015-12-01

    Full Text Available In the present work, the effect of the Abies alba essential oil in two different concentrations on oxidative stability of chicken thigh muscles during chilled storage was investigated. In the experiment were chickens of hybrid combination Cobb 500 after 42 days of the fattening period slaughtered.  All the broiler chickens were fed with the same feed mixtures and were kept under the same conditions. The feed mixtures were produced without any antibiotic preparations and coccidiostatics. After slaughtering was dissection obtained fresh chicken thigh with skin from left half-carcass which were divided into five groups (n = 5: C - control air-packaged group; A1 - vacuum-packaged experimental group; A2 - vacuum-packaged experimental group with ethylenediaminetetraacetic acid (EDTA solution 1.50% w/w; A3 - vacuum-packaged experimental group with Abies alba oil 0.10% v/w and A4 - vacuum-packaged experimental group with Abies alba oil 0.20% v/w. The Abies alba essential oil was applicate on ground chicken things and immediately after dipping, each sample was packaged using a vacuum packaging machine and storage in refrigerate at 4 ±0.5 °C. Thiobarbituric acid (TBA value expressed in number of malondialdehyde was measured in the process of first storage day of 1st, 4th, 8th, 12th and 16th day after slaughtering and expressed on the amount of malondialdehyde (MDA in 1 kg sample. The treatments of chicken things with Abies alba essential oil show statistically significant differences between all testing groups and control group, where higher average value of MDA measured in thigh muscle of broiler chickens was in samples of control group (0.4380 mg.kg-1 compared to experimental groups A1 (0.124 mg.kg-1, A2 (0.086 mg.kg-1, A3 (0.082 mg.kg-1 and A4 (0.077 mg.kg-1 after 16-day of chilled storage. Experiment results show that the treatment of chicken thigh with Abies alba essential oil positively influenced on the reduction of oxidative processes in thigh

  3. Construction and characterization of a normalized cDNA library.

    OpenAIRE

    Soares, M B; Bonaldo, M F; Jelene, P; Su, L; Lawton, L; Efstratiadis, A

    1994-01-01

    We have developed a simple procedure based on reassociation kinetics that can reduce effectively the high variation in abundance among the clones of a cDNA library that represent individual mRNA species. For this normalization, we used as a model system a library of human infant brain cDNAs that were cloned directionally into a phagemid vector and, thus, could be easily converted into single-stranded circles. After controlled primer extension to synthesize a short complementary strand on each...

  4. Activation of mRNA translation by phage protein and low temperature: the case of Lactococcus lactis abortive infection system AbiD1

    Directory of Open Access Journals (Sweden)

    Ehrlich S Dusko

    2009-01-01

    Full Text Available Abstract Background Abortive infection (Abi mechanisms comprise numerous strategies developed by bacteria to avoid being killed by bacteriophage (phage. Escherichia coli Abis are considered as mediators of programmed cell death, which is induced by infecting phage. Abis were also proposed to be stress response elements, but no environmental activation signals have yet been identified. Abis are widespread in Lactococcus lactis, but regulation of their expression remains an open question. We previously showed that development of AbiD1 abortive infection against phage bIL66 depends on orf1, which is expressed in mid-infection. However, molecular basis for this activation remains unclear. Results In non-infected AbiD1+ cells, specific abiD1 mRNA is unstable and present in low amounts. It does not increase during abortive infection of sensitive phage. Protein synthesis directed by the abiD1 translation initiation region is also inefficient. The presence of the phage orf1 gene, but not its mutant AbiD1R allele, strongly increases abiD1 translation efficiency. Interestingly, cell growth at low temperature also activates translation of abiD1 mRNA and consequently the AbiD1 phenotype, and occurs independently of phage infection. There is no synergism between the two abiD1 inducers. Purified Orf1 protein binds mRNAs containing a secondary structure motif, identified within the translation initiation regions of abiD1, the mid-infection phage bIL66 M-operon, and the L. lactis osmC gene. Conclusion Expression of the abiD1 gene and consequently AbiD1 phenotype is specifically translationally activated by the phage Orf1 protein. The loss of ability to activate translation of abiD1 mRNA determines the molecular basis for phage resistance to AbiD1. We show for the first time that temperature downshift also activates abortive infection by activation of abiD1 mRNA translation.

  5. René Descartes, the Robots and the Soul in the debate between Tommaso Ceva and Guido Grandi

    Directory of Open Access Journals (Sweden)

    Alfredo Serrai

    2016-05-01

    Full Text Available Descartes outlined human intelligence as a divine gift, albeit in a system governed by universal laws of physics and mathematics. In the eighteenth century the camaldolite Guido Grandi and Tommaso Ceva, both mathematicians, had a dispute about the interpretation of the Cartesian model of human intelligence. This case provides an opportunity to retrace the path of a speculative techno-philosophical question concerning the human possibility to equipping robots with the ability to experience a self-consciousness.

  6. Development of general biomass allometric equations for Tectona grandis Linn.f. and Eucalyptus camaldulensis Dehnh. plantations in Thailand

    Directory of Open Access Journals (Sweden)

    Waraporn Ounban

    2016-01-01

    Full Text Available Some common, general biomass allometric equations were developed and tested for estimating the stem and aboveground biomass (AGB of Tectona grandis and Eucalyptus camaldulensis plantations. In total, 84 datasets for T. grandis and 94 datasets for E. camaldulensis were gathered from published papers. The general allometric equations were then developed and the slopes and elevations were tested using ANCOVA. Spacing of 2 m × 4 m, 2 m × 8 m, 3 m × 3 m and 4 m × 4 m for T. grandis and 2 m × 3 m, 2 m × 4 m, 2 m × 8 m and 3 m × 3 m for E. camaldulensis were used as control factors. The results confirmed that diameter at breast height (D and total height (H were the best parameters for biomass estimation, of which the simple combination D2H produced the best estimation. The general allometric equations which gave the best fit (p  0.05. The range of D and H was 4.4–41.2 cm and 5.5–31.0 m, respectively, for T. grandis and 0.5–19.8 cm and 1.7–26.0 m, respectively, for E. camaldulensis.

  7. [Construction and Identification of the cDNA Expression Library for Human Esophageal Cancer Cells].

    Science.gov (United States)

    Zhang, Zhe; Wu, Xiang-Yu; Feng, Lu; Huang, Shang-Ke; Luo, Min-Na; Shao, Shan; Zhao, Xin-Han

    2016-09-01

    To construct a cDNA phage expression library for human esophageal cancer cells. After the total RNA were obtained from esophageal cancer cells, the mRNA were separated with magnetic beads adsorption method, and the single-strand and double-strand cDNA were synthesized through reverse transcription. With the undesirable cDNA fragments removed, the remaining cDNA (linked with Eco R1 aptamer and phosphorylated its 5'end) combined with the carrier of T7 Select10-3b. The recombinant phage were packaged in vitro for preliminary cDNA library. PCR was used to identify the size of inserted cDNA. The constructed original cDNA phage expression library for human esophageal cancer cells was consisted of 2.01×10⁶ pfu/mL bacteriophages with a recombination rate of 100%. The length of the inserted cDNA fragments were range from 300 bp to 1 500 bp. The cDNA phage expression library of human esophageal cell is successfully constructed to meet the currently recognized standards, and can be well used to screen cDNA-cloned genes of human esophageal cancer antigens by serological analysis of recombinantly expressed cDNA clone (SEREX).

  8. Investigating inhibition of microbes inducing microbiologically-influenced-corrosion by Tectona grandis based Fe-nanoparticle material

    Science.gov (United States)

    Okeniyi, Joshua Olusegun; Omotosho, Olugbenga Adeshola; Inyang, Michael Anietie; Okeniyi, Elizabeth Toyin; Nwaokorie, Ikechi Thaddeus; Adidi, Emmanuel Amanogho; Owoeye, Taiwo Felicia; Nwakudu, Kelechukwu Chinedu; Akinlabu, Deborah Kehinde; Gabriel, Olanrewaju Oyewale; Taiwo, Olugbenga Samson; Awotoye, Olufisayo Adebola

    2017-02-01

    In this paper, inhibition of microbes inducing microbiologically-influenced-corrosion (MIC) of metals by Tectona grandis based Fe (iron) Nanoparticle material was investigated. For this, extract was obtained from the leaf of Tectona grandis and this was employed as precursor for synthesizing the Fe-nanoparticle material. From this, the synthesized plant extract based nanoparticle material was characterized using scanning electron microscopy and energy dispersive spectroscopy (SEM+EDS) instrument. The developed Fe bio-nanoparticle material was then employed for sensitivity and/or resistance study application against different strains of microbes that are known to induce microbiologically-influenced-corrosion, in metallic materials, and for this, microbial growth inhibition effect was compared with that from a commercial antibiotic employed as control. Results showed that the Tectona grandis based Fe-nanoparticle exhibited good inhibition effects on the growth of many of the MIC inducing microbes investigated. Sensitivity measures of zone of inhibition against the growth of MIC inducing microbial strains either outperformed or compares well with that obtained from the commercial antibiotic control, in the study. These results indicate positive prospect on the suitability of Fe bio-nanoparticle for corrosion inhibition applications for the protection of metals against microbiological corrosion influencing environment.

  9. Modifying solubility of polymeric xylan extracted from Eucalyptus grandis and sugarcane bagasse by suitable side chain removing enzymes.

    Science.gov (United States)

    Gomes, Katiana R; Chimphango, Annie F A; Görgens, Johann F

    2015-10-20

    α-l-Arabinofuranosidase (AbfB) and novel α-d-glucuronidase (Agu1B) enzymes were applied for selective hydrolysis of beechwood (Fagus sylvatica) xylan (Sigma-Aldrich) as well as xylans extracted from Eucalyptus grandis and sugarcane (Saccharum officinarum L.) bagasse, leading to precipitation of these carbohydrate biopolymers. Hemicellulose extraction was performed with two mild-alkali methods, Höije and Pinto. Precipitation occurred after removal of 67, 40 and 16% 4-O-methyl-d-glucuronic acid (MeGlcA) present in polymeric xylans from beechwood, E. grandis (Pinto) and E. grandis (Höije), respectively. Precipitation was maximized at Agu1B levels of 3.79-7.53mg/gsubstrate and hemicellulose concentrations of 4.5-5.0% (w/v). Polymeric xylan from sugarcane bagasse precipitated after removal of 48 and 22% of arabinose and MeGlcA, respectively, at optimal AbfB and Agu1B dosages of 9.0U/g and 6.4mg/g, respectively. Both the purity of polymeric xylans and structure thereof had a critical impact on the propensity for precipitation, and morphology of the resulting precipitate. Nano-to micro-meter precipitates were produced, with potential for carbohydrate nanotechnology applications. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Spectral characterisation, antiviral activities, in silico ADMET and molecular docking of the compounds isolated from Tectona grandis to chikungunya virus.

    Science.gov (United States)

    K, Sangeetha; Purushothaman, Indu; S, Rajarajan

    2017-03-01

    Chikungunya infection is treated symptomatically with antipyretics and anti-inflammatory drugs without any specific antiviral drug till date. The lack of an approved antiviral drug and the emergence of virulent strains after 2006 epidemics emphasize the need for the development of potential antiviral drugs to Chikungunya virus. Hence, we studied the antiviral activity of the extracts and compounds isolated from Tectona grandis leaves to both the Asian and East central South African strains of Chikungunya virus. Five compounds were isolated from the ethanolic extract of Tectona grandis by bioactivity guided fractionation followed by Spectral Characterisation through GC-MS and NMR spectroscopy and investigated for the antiviral activity. Also in silico ADMET and Molecular Docking of the characterised compounds against the structural and non structural proteins of Chikungunya virus were performed. The characterised compound Benzene-1-carboxylic acid hexadeconate was effective at IC 50 3.036μg/ml (7.5μM) and 76.46μg/ml (189.02μM) to Asian and ECSA strain of CHIKV respectively. The compound showed desirable pharmacokinetic properties and significant molecular interactions with the E1 protein of Chikungunya virus by in silico analysis. Thus Benzene-1-carboxylic acid-2-hexadeconate isolated from Tectona grandis was found to be a promising drug candidate to both the Asian and ECSA strains of Chikungunya virus with high selectivity indices in comparison to the reference RNA antiviral drug Ribavirin. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  11. Evaluation of the present genetic conservation efforts in Pinus sylvestris, Picea abies, Quercus spp., Fagus sylvatica, and Pinus pinaster

    NARCIS (Netherlands)

    Kramer, K.

    2015-01-01

    Information on genetic diversity and gene conservation activities were combined with climatic data to evaluate the present genetic conservation efforts in Pinus sylvestris, Picea abies, Quercus spp., Fagus sylvatica, and Pinus pinaster. Combinations of climatic variables explained much of the

  12. High level of variation at Abies alba chloroplast microsatellite loci in Europe.

    Science.gov (United States)

    1999-07-01

    Based on two polymorphic chloroplast microsatellites that had been previously identified and sequence characterized in the genus Abies, genetic variation was studied in a total of 714 individuals from 17 European silver fir (Abies alba Mill.) populations distributed all over the natural range. We found eight and 18 different length variants at each locus, respectively, which combined into 90 different haplotypes. Genetic distances between most populations were high and significant. There is also evidence for spatial organization of the distribution of haplotypes, as shown by permutation tests, which demonstrate that genetic distances increase with spatial distances. A large heterogeneity in levels of diversity across populations was observed. Furthermore, there is good congruence in the levels of allelic richness of the two loci across populations. The present organization of levels of allelic richness across the range of the species is likely to have been shaped by the distribution of refugia during the last glaciation and the subsequent recolonization processes.

  13. Abies semenovii B. Fedtsch. at the Peter the Great Botanical Garden

    Directory of Open Access Journals (Sweden)

    Tkachenko Kirill

    2016-12-01

    Full Text Available Abies semenovii B. Fedtsch. (Pinaceae is an extremely rare flora species of the Central Asia (Kirghizia; it has been cultivated at the Peter the Great Botanical Garden of the Komarov Botanical Institute of the Russian Academy of Sciences (RAS since 1949, where it was first introduced into general cultivation. Since 2000, upon reaching the age of 43 years, the seed reproduction of the plants is being marked. An X-ray test proved seeds, collected in 2014, to be filled and full. In spring 2015, first time in the 67 years of cultivating this specie in St. Petersburg area, first young crops were received. Abies semenovii – a cold hard and decorative tree – has to be introduced into the gardening of St. Petersburg and shall be promoted into the Karelia and further to the northern regions of the European part of the Russian Federation.

  14. Metabolite changes in conifer buds and needles during forced bud break in Norway spruce (Picea abies) and European silver fir (Abies alba).

    Science.gov (United States)

    Dhuli, Priyanka; Rohloff, Jens; Strimbeck, G Richard

    2014-01-01

    Environmental changes such as early spring and warm spells induce bud burst and photosynthetic processes in cold-acclimated coniferous trees and consequently, cellular metabolism in overwintering needles and buds. The purpose of the study was to examine metabolism in conifers under forced deacclimation (artificially induced spring) by exposing shoots of Picea abies (boreal species) and Abies alba (temperate species) to a greenhouse environment (22°C, 16/8 h D/N cycle) over a 9 weeks period. Each week, we scored bud opening and collected samples for GC/MS-based metabolite profiling. We detected a total of 169 assigned metabolites and 80 identified metabolites, comprising compounds such as mono- and disaccharides, Krebs cycle acids, amino acids, polyols, phenolics, and phosphorylated structures. Untargeted multivariate statistical analysis based on PCA and cluster analysis segregated samples by species, tissue type, and stage of tissue deacclimations. Similar patterns of metabolic regulation in both species were observed in buds (amino acids, Krebs cycle acids) and needles (hexoses, pentoses, and Krebs cycle acids). Based on correlation of bud opening score with compound levels, distinct metabolites could be associated with bud and shoot development, including amino acids, sugars, and acids with known osmolyte function, and secondary metabolites. This study has shed light on how elevated temperature affects metabolism in buds and needles of conifer species during the deacclimation phase, and contributes to the discussion about how phenological characters in conifers may respond to future global warming.

  15. Light acclimation of photosynthesis in two closely related firs (Abies pinsapo Boiss. and Abies alba Mill.): the role of leaf anatomy and mesophyll conductance to CO2.

    Science.gov (United States)

    Peguero-Pina, José Javier; Sancho-Knapik, Domingo; Flexas, Jaume; Galmés, Jeroni; Niinemets, Ülo; Gil-Pelegrín, Eustaquio

    2016-03-01

    Leaves growing in the forest understory usually present a decreased mesophyll conductance (gm) and photosynthetic capacity. The role of leaf anatomy in determining the variability in gm among species is known, but there is a lack of information on how the acclimation of gm to shade conditions is driven by changes in leaf anatomy. Within this context, we demonstrated that Abies pinsapo Boiss. experienced profound modifications in needle anatomy to drastic changes in light availability that ultimately led to differential photosynthetic performance between trees grown in the open field and in the forest understory. In contrast to A. pinsapo, its congeneric Abies alba Mill. did not show differences either in needle anatomy or in photosynthetic parameters between trees grown in the open field and in the forest understory. The increased gm values found in trees of A. pinsapo grown in the open field can be explained by occurrence of stomata at both needle sides (amphistomatous needles), increased chloroplast surface area exposed to intercellular airspace, decreased cell wall thickness and, especially, decreased chloroplast thickness. To the best of our knowledge, the role of such drastic changes in ultrastructural needle anatomy in explaining the response of gm to the light environment has not been demonstrated in field conditions. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  16. Metabolite changes in conifer buds and needles during forced bud break in Norway spruce (Picea abies and European silver fir (Abies alba

    Directory of Open Access Journals (Sweden)

    Priyanka eDhuli

    2014-12-01

    Full Text Available Environmental changes such as early spring and warm spells induce bud burst and photosynthetic processes in cold-acclimated coniferous trees and consequently, cellular metabolism in overwintering needles and buds. The purpose of the study was to examine metabolism in conifers under forced deacclimation (artificially induced spring by exposing shoots of Picea abies (boreal species and Abies alba (temperate species to a greenhouse environment (22°C, 16/8 h D/N cycle over a nine week period. Each week, we scored bud opening and collected samples for GC/MS–based metabolite profiling. We detected a total of 169 assigned metabolites and 80 identified metabolites, comprising compounds such as mono- and disaccharides, Krebs cycle acids, amino acids, polyols, phenolics and phosphorylated structures. Untargeted multivariate statistical analysis based on PCA and cluster analysis segregated samples by species, tissue type, and stage of tissue deacclimations. Similar patterns of metabolic regulation in both species were observed in buds (amino acids, Krebs cycle acids and needles (hexoses, pentoses, and Krebs cycle acids. Based on correlation of bud opening score with compound levels, distinct metabolites could be associated with bud and shoot development, including amino acids, sugars and acids with known osmolyte function, and secondary metabolites. This study has shed light on how elevated temperature affects metabolism in buds and needles of conifer species during the deacclimation phase, and contributes to the discussion about how phenological characters in conifers may respond to future global warming.

  17. Characterization of a cDNA encoding cottonseed catalase.

    Science.gov (United States)

    Ni, W; Turley, R B; Trelease, R N

    1990-06-21

    A 1.7 kb cDNA clone was isolated from our lambda gt11 library constructed from poly(A) RNA of 24-h-old cotyledons. The cDNA encodes a full-length catalase peptide (492 amino acid residues). The calculated molecular mass is 56,800, similar to that determined for purified enzyme (57,000 SDS-PAGE). Among higher plant catalases, this cotton catalase shows the highest amino acid sequence identity (85%) to the subunit of homotetrameric maize CAT 1, a developmental counterpart to the homotetrameric CAT A isoform of cotton seeds. Comparison of sequences from cotton, sweet potato, maize CAT 1, and yeast with bovine catalase revealed that the amino acid residues and regions that are involved in catalytic activity and/or required to maintain basic catalase structure, are highly conserved. The C-terminus region, which has the lowest nucleotide sequence identity between plant and mammalian catalases, does not terminate with a tripeptide, S-K/R/H-L, a putative targeting signal for peroxisomal proteins.

  18. Infectious Maize rayado fino virus from Cloned cDNA.

    Science.gov (United States)

    Edwards, Michael C; Weiland, John J; Todd, Jane; Stewart, Lucy R

    2015-06-01

    A full-length cDNA clone was produced from a U.S. isolate of Maize rayado fino virus (MRFV), the type member of the genus Marafivirus within the family Tymoviridae. Infectivity of transcripts derived from cDNA clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors Dalbulus maidis and Graminella nigrifrons that transmit the virus in a persistent-propagative manner. Infection of maize plants through vascular puncture inoculation of seed with transcript RNA resulted in the induction of fine stipple stripe symptoms typical of those produced by wild-type MRFV and a frequency of infection comparable with that of the wild type. Northern and Western blotting confirmed the production of MRFV-specific RNAs and proteins in infected plants and protoplasts. An unanticipated increase in subgenomic RNA synthesis over levels in infected plants was observed in protoplasts infected with either wild-type or cloned virus. A conserved cleavage site motif previously demonstrated to function in both Oat blue dwarf virus capsid protein and tymoviral nonstructural protein processing was identified near the amino terminus of the MRFV replicase polyprotein, suggesting that cleavage at this site also may occur.

  19. Comparison of Flavonoid Compounds in the Flavedo and Juice of Two Pummelo Cultivars (Citrus grandis L. Osbeck from Different Cultivation Regions in China

    Directory of Open Access Journals (Sweden)

    Mingxia Zhang

    2014-10-01

    Full Text Available The objective of this study was to investigate the effect of different cultivation regions on the pattern and content of flavonoids in two pummelo cultivars (C. grandis L. Osbeck in China. Results showed that similar patterns of flavonoids were observed in the flavedo or juice of each pummelo cultivar from these cultivation regions, whereas the individual flavonoid content showed unique characteristics. Naringin, the predominant flavanone glycoside, showed the highest content in both flavedo and juice of C. grandis “Guanximiyu” from the Pinghe of Fujian (FJ cultivation region compared with the Dapu of Guangdong (GD and Nanbu of Sichuan (SC regions. However, its content in the flavedo of C. grandis “Shatianyu” from the Pingle of Guangxi (GX was significantly lower than in the GD and SC regions. Vicenin-2 appeared to be the dominant flavone C-glycoside in the flavedo of both cultivars, and the lowest content was observed in the flavedo of C. grandis “Guanximiyu” from the SC region. However, C. grandis “Shatianyu” contained the highest content of vicenin-2 in the flavedo from SC region. Similarly, the predominant flavone O-glucoside, rhoifolin, showed the highest content in C. grandis “Guanximiyu” from the GD and FJ regions, whereas C. grandis “Shatianyu” in SC region showed the highest content of rhoifolin. Cluster analysis suggested that genotype played a primary role in determining the flavonoid profiles of pummelo cultivars, whereas regional differences significantly affected the flavonoid distribution of pummelo cultivars potentially via affecting the direction of flavonoid accumulation in pummelo.

  20. Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species

    OpenAIRE

    Sánchez Robles, José Manuel; Talavera Lozano, Salvador; García Castaño, Juan Luis; Terrab Benjelloun, Anass; Navarro Sampedro, Laura; Balao Robles, Francisco J.

    2012-01-01

    Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other ...

  1. Chrysophycean stomatocysts from Morskie Oko and Żabie Oko lakes in the Tatra National Park, Poland

    Directory of Open Access Journals (Sweden)

    Jolanta Cabała

    2011-01-01

    Full Text Available Sixteen chrysophycean stomatocysts are reported from the lakes Morskie Oko and Żabie Oko in the Tatra National Park, Poland. Of these, six morphotypes are new to Poland, and two morphotypes plus one forma are new to science. These stomatocysts are illustrated with SEM micrographs and described according to International Statospore Working Group (ISWG guidelines. The comparison of stomatocyst community between Morskie Oko and Żabie Oko lakes is given.

  2. Large-scale transcriptional profiling of lignified tissues in Tectona grandis.

    Science.gov (United States)

    Galeano, Esteban; Vasconcelos, Tarcísio Sales; Vidal, Mabel; Mejia-Guerra, Maria Katherine; Carrer, Helaine

    2015-09-15

    Currently, Tectona grandis is one of the most valuable trees in the world and no transcript dataset related to secondary xylem is available. Considering how important the secondary xylem and sapwood transition from young to mature trees is, little is known about the expression differences between those successional processes and which transcription factors could regulate lignin biosynthesis in this tropical tree. Although MYB transcription factors are one of the largest superfamilies in plants related to secondary metabolism, it has not yet been characterized in teak. These results will open new perspectives for studies of diversity, ecology, breeding and genomic programs aiming to understand deeply the biology of this species. We present a widely expressed gene catalog for T. grandis using Illumina technology and the de novo assembly. A total of 462,260 transcripts were obtained, with 1,502 and 931 genes differentially expressed for stem and branch secondary xylem, respectively, during age transition. Analysis of stem and branch secondary xylem indicates substantial similarity in gene ontologies including carbohydrate enzymes, response to stress, protein binding, and allowed us to find transcription factors and heat-shock proteins differentially expressed. TgMYB1 displays a MYB domain and a predicted coiled-coil (CC) domain, while TgMYB2, TgMYB3 and TgMYB4 showed R2R3-MYB domain and grouped with MYBs from several gymnosperms and flowering plants. TgMYB1, TgMYB4 and TgCES presented higher expression in mature secondary xylem, in contrast with TgMYB2, TgHsp1, TgHsp2, TgHsp3, and TgBi whose expression is higher in young lignified tissues. TgMYB3 is expressed at lower level in secondary xylem. Expression patterns of MYB transcription factors and heat-shock proteins in lignified tissues are dissimilar when tree development was evaluated, obtaining more expression of TgMYB1 and TgMYB4 in lignified tissues of 60-year-old trees, and more expression in TgHsp1, TgHsp2, Tg

  3. Genetic diversity of Spanish fir (Abies pinsapo Boiss.) populations by means of megagametophyte storage proteins

    OpenAIRE

    Martín, María Angela; Alvarez, Juan Bautista; Martín, Luís Miguel

    2010-01-01

    International audience; The Spanish fir (Abies pinsapo Boiss.) is a conifer endemic to southern Spain and belongs to a Mediterranean basin group of firs considered to be relict. Megagametophyte storage proteins are a useful tool for the evaluation of genetic variability due to the haploid nature of megagametophytes in conifers.* In this paper, we report for the first time, the usefulness of megagametophyte storage proteins marker in A. pinsapo as a first investigation of the level of genetic ...

  4. Composition of essential oil from seeds and cones of Abies alba.

    Science.gov (United States)

    Wajs, Anna; Urbańska, Justyna; Zaleśkiewicz, Ewa; Bonikowski, Radosław

    2010-08-01

    The volatile composition of Abies alba Mill. seeds and cone scales has been studied, leading to the determination of 90 volatile constituents. The major component of the seed essential oil was (-)-limonene (about 70%), while that of the cone scale oil was a-pinene (57%). Monoterpene hydrocarbons were predominant in both oils, but the quantitative and qualitative composition of the volatile compounds was specific for each part of the tree.

  5. Influence of ultrasounds on some mechanical properties of fir wood (Abies alba Mill). Microscopic sections

    Science.gov (United States)

    Parpala, V.; Pastirnac, A.; Paraschiv, N.

    1974-01-01

    It was found that as a result of ultrasonic treatment fir wood (Abies alba Mill) shows the effects of homogenization, and the modulus of elasticity for static flexure drops on an average by 50% for 45 min of treatment. The drop is more pronounced for test pieces with 8 to 12 annual rings. Study of microscopic sections disclosed that early wood with one annual ring undergoes the most powerful effect.

  6. Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

    OpenAIRE

    Teresa Hazubska-Przybył; Krystyna Bojarczyk

    2011-01-01

    Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. brewenana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. brewenana) to 23.75% (P. omorika and P. pungens 'Glauca'). The frequency of embryogenic tissue initiation was strongly affected by medium ...

  7. Chemodiversity of the Essential Oil from Leaves of Abies nebrodensis (Lojac.) Mattei.

    Science.gov (United States)

    Schicchi, Rosario; Geraci, Anna; Rosselli, Sergio; Maggio, Antonella; Bruno, Maurizio

    2017-02-01

    Abies nebrodensis (Lojac.) Mattei (Pinaceae) is a species occurring in a very small population only in a restricted area of Sicily. Its taxonomic classification as different species has been object of discussion. In this work the chemical composition of the essential oil from the leaves is presented for the first time and compared to the essential oils from other euroasiatic species reported in literature. Peculiar characteristics of the essential oil of A. nebrodensis are highlighted. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  8. Massive decline of Cystoseira abies-marina forests in Gran Canaria Island (Canary Islands, eastern Atlantic

    Directory of Open Access Journals (Sweden)

    José Valdazo

    2017-12-01

    Full Text Available Brown macroalgae within the genus Cystoseira are some of the most relevant “ecosystem-engineers” found throughout the Mediterranean and the adjacent Atlantic coasts. Cystoseira-dominated assemblages are sensitive to anthropogenic pressures, and historical declines have been reported from some regions. In particular, Cystoseira abies-marina, thriving on shallow rocky shores, is a key species for the ecosystems of the Canary Islands. In this work, we analyse changes in the distribution and extension of C. abies-marina in the last decades on the island of Gran Canaria. This alga dominated the shallow rocky shores of the entire island in the 1980s; a continuous belt extended along 120.5 km of the coastline and occupied 928 ha. In the first decade of the 21st century, fragmented populations were found along 52.2 km of the coastline and occupied 12.6 ha. Today, this species is found along 37.8 km of the coastline and occupies only 7.4 ha, mainly as scattered patches. This regression has been drastic around the whole island, even in areas with low anthropogenic pressure; the magnitude of the decline over time and the intensity of local human impacts have not shown a significant correlation. This study highlights a real need to implement conservation and restoration policies for C. abies-marina in this region.

  9. Final Scientific/Technical report for "ABI8: Prototype of a novel signaling factor"

    Energy Technology Data Exchange (ETDEWEB)

    Finkelstein, Ruth R. [Univ. of California, Santa Barbara, CA (United States)

    2013-02-21

    The Arabidopsis thaliana ABSCISIC ACID-INSENSITIVE8 locus encodes a highly conserved plant-specific protein that mediates abscisic acid (ABA) and sugar responses essential for growth. Although initial database comparisons revealed no domains of predictable function, it has recently been re-annotated as a member of the Glycosyltransferase family A. However, this function has not been demonstrated experimentally and no specific substrates have been identified. Mutations affecting ABI8 are near-lethal due to pleiotropic yet specific effects including altered ABA signaling, sugar transport, cell wall synthesis, root meristem maintenance, vascular patterning, and male sterility. Because the predicted sequence initially provided no clues, we used a guilt by association strategy to address function of this protein by determining its subcellular localization and identifying interacting proteins. Our studies showed that ABI8 is localized to the endomembrane system and may interact with proteins implicated in Golgi trafficking, lignification, and stress signaling. We found that the root meristem arrest reflects decreased auxin accumulation and resulting decreases in regulators required for meristem identity, all of which can be rescued by added glucose. Further studies showed that this glucose-dependence reflects reduced glucose uptake as well as the decreased expression of sugar-mobilizing enzymes. This work suggests that ABI8 may regulate trafficking of membrane proteins such as auxin transporters and cellulose synthase, but this hypothesis has not yet been tested. The altered gene expression is likely to be a secondary or later effect of this pleiotropic mutation.

  10. Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana

    Directory of Open Access Journals (Sweden)

    Teresa Hazubska-Przybył

    2011-01-01

    Full Text Available Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. brewenana to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. brewenana to 23.75% (P. omorika and P. pungens 'Glauca'. The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram and sucrose concentration (10-20 g-1"1. A lower frequency was obtained in Picea omorika (10% when megagametophytes (endosperms with immature zygotic embryos were used as explants. No emryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%. The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.

  11. Preparation of full-length cDNA libraries: focus on metazoans.

    Science.gov (United States)

    Harada, Masako; Hayashizaki, Yoshihide

    2009-01-01

    Critical steps in a cDNA library preparation include efficient cDNA synthesis, selection of full-length cDNAs, normalizing their abundance, and the subtraction of redundant transcripts. The use of trehalose and sorbiol stabilizes the activity of the reverse transcriptase leading to efficient cDNA synthesis and the cap-trapping method is used for efficient full-length cDNA selection. Through the incorporation of additional normalization and subtraction steps that eliminate the size bias and expressed gene frequency, it is possible to attain cDNA libraries that include larger or rarely expressed genes. This chapter describes an efficient method to construct a full-length cDNA library, with a focus on metazoan samples.

  12. Feeding ecology of the copepod Lucicutia aff. L. grandis near the lower interface of the Arabian Sea oxygen minimum zone

    Science.gov (United States)

    Gowing, Marcia M.; Wishner, Karen F.

    Feeding ecology of the calanoid copepod Lucicutia aff. L. grandis collected in the Arabian Sea at one station during the Spring Intermonsoon and during the Southwest Monsoon of 1995 was studied with transmission electron microscopy of gut-contents. Highest abundances of these animals occurred from ˜400 to 1100 m, near the lower interface of the oxygen minimum zone and at the inflection point where oxygen starts to increase. We expected that their gut-contents would include particles and cells that had sunk relatively undegraded from surface waters as well as those from within the oxygen minimum zone, and that gut-contents would differ between the Spring Intermonsoon and the more productive SW Monsoon. Overall, in both seasons Lucicutia aff. L. grandis was omnivorous, and consumed a variety of detrital particles, prokaryotic and eukaryotic autotrophs, gram-negative bacteria including metal-precipitating bacteria, aggregates of probable gram-positive bacteria, microheterotrophs, virus-like particles and large virus-like particles, as well as cuticle and cnidarian tissue. Few significant differences in types of food consumed were seen among life stages within or among various depth zones. Amorphous, unidentifiable material was significantly more abundant in guts during the Spring Intermonsoon than during the late SW Monsoon, and recognizable cells made up a significantly higher portion of gut-contents during the late SW Monsoon. This is consistent with the Intermonsoon as a time when organic material is considerably re-worked by the surface water microbial loop before leaving the euphotic zone. In both seasons Lucicutia aff. L. grandis had consumed what appeared to be aggregates of probable gram-positive bacteria, similar to those we had previously found in gut-contents of several species of zooplankton from the oxygen minimum zone in the eastern tropical Pacific. By intercepting sinking material, populations of Lucicutia aff. L. grandis act as a filter for carbon

  13. CARACTERÍSTICAS TECNOLÓGICAS DA MADEIRA DE ÁRVORES MATRIZES DE Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Denis L. G. Fernandes

    2004-06-01

    Full Text Available O objetivo do presente trabalho foi caracterizar árvores matrizes de Eucalyptus grandis em relação a características tecnológicas da madeira. Foram selecionadas 63 árvores matrizes, pertencentes a um povoamento comercial localizado no litoral norte do Rio Grande do Sul. As árvores foram abatidas e, com base nos dados dendrométricos, calculou-se o volume comercial com e sem casca, o fator de forma, a conicidade e a relação altura/diâmetro. Quanto à madeira, foi analisada a massa específica básica, os percentuais volumétricos de cerne, alburno e casca, as rachaduras de topo das toras e das tábuas, os empenamentos e os defeitos visuais das tábuas, tais como nós e bolsa de resina. A massa específica básica, cuja média inclui a madeira dessa espécie entre as moderadamente leves a pesadas, mostrou uma tendência decrescente entre o DAP e 25% da altura comercial e, a partir daí, crescente até 100% da mesma. O percentual volumétrico de cerne apresentou valores médios crescentes desde a base até 25% da altura comercial e, a partir daí, diminuiu até 100% da altura comercial, sendo que a média geral foi de 75,7%. O comprimento médio das rachaduras de topo em tábuas de Eucalyptus grandis foi de 63%. A alta porcentagem de rachaduras de topo foi atribuída à posição de retirada das tábuas, próxima à medula. Já a variabilidade das rachaduras de topo encontrada para as toras (CV=60% possibilita a seleção de árvores com menor tendência em apresentar esse defeito.

  14. RNA-Seq reveals complex genetic response to deepwater horizon oil release in Fundulus grandis

    Directory of Open Access Journals (Sweden)

    Garcia Tzintzuni I

    2012-09-01

    Full Text Available Abstract Background The release of oil resulting from the blowout of the Deepwater Horizon (DH drilling platform was one of the largest in history discharging more than 189 million gallons of oil and subject to widespread application of oil dispersants. This event impacted a wide range of ecological habitats with a complex mix of pollutants whose biological impact is still not yet fully understood. To better understand the effects on a vertebrate genome, we studied gene expression in the salt marsh minnow Fundulus grandis, which is local to the northern coast of the Gulf of Mexico and is a sister species of the ecotoxicological model Fundulus heteroclitus. To assess genomic changes, we quantified mRNA expression using high throughput sequencing technologies (RNA-Seq in F. grandis populations in the marshes and estuaries impacted by DH oil release. This application of RNA-Seq to a non-model, wild, and ecologically significant organism is an important evaluation of the technology to quickly assess similar events in the future. Results Our de novo assembly of RNA-Seq data produced a large set of sequences which included many duplicates and fragments. In many cases several of these could be associated with a common reference sequence using blast to query a reference database. This reduced the set of significant genes to 1,070 down-regulated and 1,251 up-regulated genes. These genes indicate a broad and complex genomic response to DH oil exposure including the expected AHR-mediated response and CYP genes. In addition a response to hypoxic conditions and an immune response are also indicated. Several genes in the choriogenin family were down-regulated in the exposed group; a response that is consistent with AH exposure. These analyses are in agreement with oligonucleotide-based microarray analyses, and describe only a subset of significant genes with aberrant regulation in the exposed set. Conclusion RNA-Seq may be successfully applied to feral and

  15. Solid-phase cDNA library construction, a versatile approach.

    OpenAIRE

    Roeder, T

    1998-01-01

    A rapid and versatile method for cDNA library construction was developed. It is based on conventional cDNA library synthesis including all enzymatic steps usually required, but is performed on a solid support. The cDNA is immobilised via a biotin residue to streptavidin coupled magnetic beads, which allows rapid and easy to perform changes of buffers and enzymes. Therefore, it combines speed (library construction within a single day) with high quality libraries, making it ideally suited for m...

  16. [Software development in data analysis and mining for cDNA microarray].

    Science.gov (United States)

    Wu, Bin; Wang, Jianguo; Wang, Miqu

    2007-12-01

    Data analysis and mining is a key issue to microarray technology and is usually implemented through software development. This paper summarizes the state-of-art software development in cDNA microarray data analysis and mining. The updated software developments are discussed in three stages: data inquisition from cDNA microarray tests, statistical treatment of cDNA data and data mining from gene network.

  17. Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA

    DEFF Research Database (Denmark)

    Kristensen, Torsten; Schousboe, Inger; Boel, Espen

    1991-01-01

    Human β2-glycoprotein (β2gpI) cDNA was isolated from a liver cDNA library and sequenced. The cDNA encoded a 19-residue hydrophobic signal peptide followed by the mature β2gpI of 326 amino acid residues. In liver and in the hepatoma cell line HepG2 there are two mRNA species of about 1.4 and 4.3 kb...

  18. Durabilidad natural de madera de Eucalyptus grandis Hill ex Maiden de plantaciones de rápido crecimiento

    Directory of Open Access Journals (Sweden)

    Silvia Böthig

    2011-05-01

    Full Text Available Una de las especies forestales de rápido crecimiento cultivadas en Uruguay de mayor importancia económica es el Eucalyptus grandis. Trabajos anteriores reportan propiedades físicas y mecánicas de la madera juvenil y adulta proveniente de plantaciones de esta especie en diferentes regiones del país. Sin embargo, dado que no se dispone de datos científicos nacionales sobre su durabilidad natural, en este trabajo fue evaluada madera de E. grandis obtenida de dos plantaciones de 16 años de edad con semilla del mismo origen, de dos sitios, Rivera y Río Negro. Se estudió la durabilidad natural del duramen externo e interno siguiendo los métodos de la norma EN 350-1, tomando Populus deltoides x euroamericana cv I-214 como especie de referencia. Se realizaron ensayos de laboratorio para determinar la resistencia a la descomposición fúngica (Gloeophyllum trabeum, Trametes versicolor y Serpula lacrymans y a las termites (Reticulitermes spp. siguiendo las normas EN 113 y EN 118, respectivamente. Se realizaron ensayos de campo de estacas, de doble capa y cámara fúngica, los cuales aún están en curso, por lo que en el presente trabajo se presentan resultados parciales. Los perfiles radiales de densidad básica revelaron que la madera del duramen externo no era adulta, sino madera de transición. En general, el duramen de E. grandis mostró una mayor durabilidad que el híbrido Populus.El E. grandis se clasificó como moderadamente o seriamente atacado por Reticulitermes spp. Según EN 350-1, la madera juvenil de E. grandis puede considerarse como “moderadamente durable” ante la pudrición parda provocada por G. trabeum, mientras que la madera de transición puede describirse como “durable”. La madera de transición mostró en relación a la madera juvenil una mayor resistencia al G. trabeum, una susceptibilidad levemente menor a las termites y mejor desempeño en el campo luego de 17 meses de exposición. El sitio de la plantación no

  19. 7 CFR 301.92-2 - Restricted, regulated, and associated articles; lists of proven hosts and associated plant taxa.

    Science.gov (United States)

    2010-01-01

    ... Phytophthora ramorum: Abies concolor White fir Abies grandis Grand fir Abies magnifica Red fir Acer circinatum... yew Taxus x media Yew Torreya californica California nutmeg Toxicodendron diversilobum Poison oak...

  20. Functional cloning using pFB retroviral cDNA expression libraries.

    Science.gov (United States)

    Felts, Katherine A; Chen, Keith; Zaharee, Kim; Sundar, Latha; Limjoco, Jamie; Miller, Anna; Vaillancourt, Peter

    2002-09-01

    Retroviral cDNA expression libraries allow the efficient introduction of complex cDNA libraries into virtually any mitotic cell type for screening based on gene function. The cDNA copy number per cell can be easily controlled by adjusting the multiplicity of infection, thus cell populations may be generated in which >90% of infected cells contain one to three cDNAs. We describe the isolation of two known oncogenes and one cell-surface receptor from a human Burkitt's lymphoma (Daudi) cDNA library inserted into the high-titer retroviral vector pFB.

  1. WOOD BASIC DENSITY EFFECT OF Eucalyptus grandis x Eucalyptus urophylla CLONES ON BLEACHED PULP QUALITY

    Directory of Open Access Journals (Sweden)

    Sheila Rodrigues dos Santos

    2010-08-01

    Full Text Available The study analyzed the wood basic density effect in two Eucalyptus grandis x Eucalyptus urophylla hybrid clones (440 kg/m3 e 508 kg/m3 on bleached pulp quality (fiber dimensions and physical-mechanical properties. The woods performance on pulping, bleaching and beating results were analyzed. The Kraft pulping was carried out in forced circulation digester in order to obtain 17±1 kappa number targets. The pulps were bleached to 90±1 using delignification oxygen and D0EOPD1 bleaching sequence. Bleached pulp of low basic density clone showed, significantly, lowest revolutions number in the PFI mill to reach tensile index of 70 N.m/g, low Schopper Riegler degree and generated sheets with higher values to bulk and opacity. These characteristics and properties allow concluding that bleached pulp of low basic density clone was the most indicated to produce printing and writing sheets. The bleached pulp of high basic density clone showed higher values of bulk and capillarity Klemm and lower water retention value when analyzed without beating. The bleached pulp of high basic density clone showed more favorable characteristics to the production of tissue papers.

  2. Estimates of genetic parameters of wood traits for sawn timber production in Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Paulo Eduardo Telles dos Santos

    2004-01-01

    Full Text Available In this study, the breeding perspectives of 41 open-pollinated progenies of Eucalyptus grandis were evaluated based on their wood traits. The progenies were distributed in two experiments in a randomized complete block design, with three replicates and linear plots containing six plants each. The traits were assessed at eight years of age. Two trees from each plot were selected for this assessment based on better growth, stem form and phytosanity. Significant differences in basic density, sapwood/heartwood ratio, bowing, specific gravity, parallel compression and static bending were detected among the progenies. These traits were potentially promising for breeding programs, with heritability coefficients that varied from 0.34 to 0.61 on a progeny mean basis. There was no genetic variation in the moisture content, board end-splitting, log volume under bark, log eccentricity, bark content, crooking, and shear strength of the progenies. Intermediate to highly significant genetic correlations were detected among the physical and mechanical properties, as well as between pairs of traits such as basic density and log end-splitting, basic density and bowing, specific gravity and bowing, sapwood/heartwood ratio and bowing, log volume and bowing, and log volume and log end-splitting. These results show that the levels of growth stress in trees can be reduced by selection using indirect traits such as the sapwood/heartwood ratio and bowing.

  3. EFFECT OF Eucalyptus grandis WOOD REFINING ON THE FIBER MORFHOLOGY AND ON MDF PANELS PROPERTIES

    Directory of Open Access Journals (Sweden)

    Ugo Leandro Belini

    2008-09-01

    Full Text Available The utilization of eucalypts wood for MDF panels producing is recent in Brazil and presents larger perspective of increase, in relationship to pine wood, due to the shorter harvesting cycle and integral use of the bark in the productive process, etc,. The present work evaluated the anatomical structure and properties of MDF panels manufactured at laboratory with Eucalyptus grandis fibers obtained in three treatments. It were evaluated the variations in heating time, digester and refining pressure and specific energy. MDF panels obtained in critical refining condition showed darker fibers and cell walls transversely broken and lower resistance to internal bond, surface soundness, withdrawal of screws and swelling in thickness. MDF panels obtained after less intensive refining condition showed better physical properties. Better refining conditions increase the bulk density and hinder the adhesive recover and fiber interlace, with worse physical and mechanics properties. Fiber morphology was evaluated through stereoscope and scanning electronic microscopic (SEM, establishing relationships between chips refining treatments and MDF panels properties.

  4. Estudo da compatibilidade entre a madeira e as cascas de Eucalyptus grandis e cimento portland.

    Directory of Open Access Journals (Sweden)

    Fábio Akira Mori

    2010-08-01

    Full Text Available As características dos painéis cimento-madeira podem ser prejudicadas em virtude da incompatibilidade química do cimento com alguns materiais lignocelulósicos que podem inibir a "pega" do cimento. Porém, este efeito pode ser minimizado com tratamentos químicos dos materiais. As diferentes espécies de Eucalyptus podem ser promissoras como matéria-prima na produção desse tipo de painel, principalmente os resíduos gerados na forma de cascas. O objetivo deste trabalho foi avaliar a compatibilidade química da madeira e cascas tratadas e não-tratadas quimicamente de Eucalyptus com o cimento Portland. O tratamento químico das cascas foi realizado com solução de hidróxido de sódio. Os resultados obtidos mostraram que a madeira de Eucalyptus grandis apresentou uma aptidão moderada ao cimento, as cascas tratadas, alta aptidão e as cascas não-tratadas, aptidão extremamente baixa. Verificou-se a influência positiva do tratamento químico nas cascas, possibilitando futuramente sua incorporação na manufatura de painéis cimento-madeira.

  5. Wood quality of Eucalyptus grandis dominant and average trees submitted to drying

    Directory of Open Access Journals (Sweden)

    Joel Telles de Souza

    2012-03-01

    Full Text Available This study determined, in trees of dominant and average diameter, the influence of air seasoning followed by kiln drying in the sawn wood quality of 14 years old stand of Eucalyptus grandis W. Hill ex Maiden, located in Capivari do Sul municipality, state of Rio Grande do Sul. The sampling of trees was performed after a pilot inventory on the basis of the dominant and average diameter at breast height (DBH. After cutting down the trees, there were selected the logs located between the DBH and 25% of commercial height. The logs were then sawn using into boards that were submitted to air seasoning from green up to 18% of moisture content, followed by kiln drying until 13% of moisture content. End checking and board crook were evaluate after sawing, following air seasoning and kiln drying. The results indicate that, both for dominant and average trees, end checking and board crook were not influenced by different wood process stage. However, the contrast between dominant and average trees shows that the magnitude of defects is higher in the average trees.

  6. Dominant Height Model for Site Classification of Eucalyptus grandis Incorporating Climatic Variables

    Directory of Open Access Journals (Sweden)

    José Roberto Soares Scolforo

    2013-01-01

    Full Text Available This study tested the effects of inserting climatic variables in Eucalyptus grandis as covariables of a dominant height model, which for site index classification is usually related to age alone. Dominant height values ranging from 1 to 12 years of age located in the Southeast region of Brazil were used, as well as data from 19 automatic meteorological stations from the area. The Chapman-Richards model was chosen to represent dominant height as a function of age. To include the environmental variables a modifier was included in the asymptote of the model. The asymptote was chosen since this parameter is responsible for the maximum value which the dominant height can reach. Of the four environmental variables most responsible for database variation, the two with the highest correlation to the mean annual increment in dominant height (mean monthly precipitation and temperature were selected to compose the asymptote modifier. Model validation showed a gain in precision of 33% (reduction of the standard error of estimate when climatic variables were inserted in the model. Possible applications of the method include the estimation of site capacity in regions lacking any planting history, as well as updating forest inventory data based on past climate regimes.

  7. Structure and function of the digestive system of solen grandis dunker

    Science.gov (United States)

    Xiuzhen, Sheng; Wenbin, Zhan; Sulian, Ren

    2003-10-01

    Structure and function of the digestive system of a bivalve mollusc, Solen grandis, were studied using light microscopy and histochemical methods. The wall of digestive tube consists of four layers: the mucosal epithelium, connective tissue, muscular and fibrosa or serosa (only in the portion of rectum) from the inner to the outer. The ciliated columnar epithelial cells, dispersed by cup-shaped mucous cells, rest on a thin base membrane. There are abundant blood spaces in connective tissue layer. The digestive diverticula are composed of multi-branched duct and digestive tubules. The digestive tubules are lined with digestive and basophilic secretory cells, and surrounded by a layer of smooth muscle fibers and connective tissues. Activities of acid and alkaline phosphatases, esterase and lipase are detected in the digestive cells, and the epithelia of stomach and intestine, suggesting that these cells are capable of intracellular digesting of food materials and absorbing. Besides, acid phosphatase and esterase activities are present in the posterior portion of esophagus. Phagocytes are abundant in blood spaces and the lumens of stomach and intestine, containing brown granules derived from the engulfed food materials. The present work indicates that phagocytes play important roles in ingestion and digestion of food materials, which is supported as well by the activities of acid phosphatase, esterase and lipase detected in blood spaces.

  8. Leaf area index estimation of Eucalyptus grandis W.Hill. in plantations

    Directory of Open Access Journals (Sweden)

    Dubal Papamija-Muñoz

    2012-12-01

    Full Text Available We estimated leaf area index (LAI in Eucalyptus grandis W.Hill. plantations in four farms in the Smurfit Kappa Carton de Colombia (SKCC with three farms located in the city of Popayan (Cauca and one located in the municipality of Restrepo (Valle del Cauca. Each farm had three fertilized and three unfertilized plots with 64 individuals in each. We used three methods, Plant Canopy Analyzer 2000 (PCA 2000, flat photograph PIPEcv software and a destructive method, which was generated using a mathematical model. The first two methods were measured bimonthly for a year and the final method required trees being cut to measure their diameter. Estimation of leaf area index was 2.01 for PCA 2000, 3.12 for PIPEcv and 2.83 for the mathematical model. These values correspond to the average and range of leaf area indices obtained for each method on all farms. Statistically the three methodologies developed in this study were not closely related.

  9. PARTICLEBOARDS PRODUCED WITH Eucalyptus grandis W. Hill ex Maiden POLYSTYRENE AND POLYETHYLENE THEREPHTHALATE PARTICLES

    Directory of Open Access Journals (Sweden)

    Antônio da Silva Maciel

    2004-06-01

    Full Text Available This research investigated the properties of wood particleboards, containing particles of polystyrene (PS and polyethylene therephthalate (PET. Particleboards were produced with 0%, 25% or 50% of polystyrene and 100%, 75% or 50% of Eucalyptus grandis particles. Additional boards were produced with two amounts of PET/PS (5/20% or 10/40% and 75% or 50% wood particles. As binding agents three amounts (0%, 4% or 6% of urea-formaldehyde or phenol-formaldehyde adhesive and three amounts of polystyrene in toluene solution (0%, 4% or 6% were used. One-layer boards, measuring approximately 400,0 x 400,0 x 10,0 mm with density approximately equal to 0,60 g/cm3, were produced. Internal bond, modulus of rupture and elasticity, screw withdrawal, as well water absorption and thickness swelling, after 24 hours of immersion were determined. All mechanical boards’ properties were superior to the established by ANSI/A 208.1-1993 standards. All wood/plastic particleboards absorbed more water then those observed in commercial boards. However the observed thickness swelling was quite similar to the values of commercial wood particleboards. Boards in which the solution of polystyrene was applied were, in general, presented the best values for all properties.

  10. Applying Sewage Sludge to Eucalyptus grandis Plantations: Effects on Biomass Production and Nutrient Cycling through Litterfall

    International Nuclear Information System (INIS)

    Da Silva, P.H.M.; Poggiani, F.; Laclau, J.P.

    2011-01-01

    In most Brazilian cities sewage sludge is dumped into sanitary landfills, even though its use in forest plantations as a fertilizer and soil conditioner might be an interesting option. Sewage sludge applications might reduce the amounts of mineral fertilizers needed to sustain the productivity on infertile tropical soils. However, sewage sludge must be applied with care to crops to avoid soil and water pollution. The aim of our study was to assess the effects of dry and wet sewage sludges on the growth and nutrient cycling of Eucalyptus grandis plantations established on the most common soil type for Brazilian eucalypt plantations. Biomass production and nutrient cycling were studied over a 36-month period in a complete randomized block design. Four experimental treatments were compared: wet sewage sludge, dry sludge, mineral fertilizer, and no fertilizer applications. The two types of sludges as well as mineral fertilizer increased significantly the biomass of Eucalyptus trees. Wood biomass productions 36 months after planting were similar in the sewage sludge and mineral fertilization treatments (about 80 tons ha - '1) and 86 % higher than in the control treatment. Sewage sludge application also affected positively leaf litter production and significantly increased nutrient transfer among the components of the ecosystem.

  11. Laser light interaction for texture evaluation of native woods and Eucalyptus grandis W. Hill ex Maiden

    International Nuclear Information System (INIS)

    Silva, M.R.; Mori, F.A.; Rabelo, G.F.; Braga Junior, R.A.

    2005-01-01

    This work had the objective of study in patterns of the ''speckle'' as function of the wood texture of Bowdichia virgilioides check for this species in other resources (sucupira), Swietenia macrophylla check for this species in other resources (mogno), Balfourodendron riedelianum check for this species in other resources (paumarfim) and Eucalyptus grandis wood. The He-Ne laser 10 mW was used to illuminate the wood samples and a digital camera captured the result of the interaction of the wood with the laser light. The results obtained from image analysis were compared with the pattern obtained by microscopic methodology. The captured images show the intensities of each pixel, varying between 0 and 255 in the gray scale. From the images, a central area of 16x16 pixels was removed and analyzed by the application of the convolution function and its Fourier Transform, resulting in the power density spectrum. The results showed that the information obtained by the power density spectrum of the ''speckle'' space variation allow classing samples of woods with different texture [pt

  12. Hygroscopicity of wood from Eucalyptus grandis and Pinus taeda subjected to thermal treatment

    Directory of Open Access Journals (Sweden)

    Karina Soares Modes

    2013-03-01

    Full Text Available This study aimed to investigate the effect of the thermal treatment technique under two conditions—autoclave combined with electric oven and electric oven only—on the hygroscopic properties of wood from Pinus taeda L. and Eucalyptus grandis W. Hill ex Maiden, comparing them with results obtained for untreated wood. Three trees at age 25 years were sampled, with boards being removed at DBH level in order to make specimens 2.5 x 2.5 x 5.0 cm in size in the radial, tangential and axial direction respectively. In the combined treatment, specimens were subjected to thermal treatment in an autoclave set at 130°C/± 3°C and kgf/cm² pressure for 3 hours, then to a conditioning period, followed by heat in an electric oven set at 160°C/±1°C for the same time. Properties being evaluated included water absorption rate, volumetric swelling, water repellency effectiveness and anti-swelling efficiency. Results revealed that the thermal treatments being adopted were effective in reducing hygroscopicity and in increasing the dimensional stability of both species, revealing also that the combined autoclave and electric oven treatment provides best results.

  13. LOG BREAKDOWN OF Eucalyptus grandis WITH CHAINSAW, PART 1 – OPERATIONAL PERFORMANCE ANALYSISi

    Directory of Open Access Journals (Sweden)

    Djeison Cesar Batista

    2013-08-01

    Full Text Available http://dx.doi.org/10.5902/1980509810558The aim of this work was to analyze the operational performance of chainsaw log breakdown of Eucalyptus grandis W.Hill, taking into account small scale use, e.g., in small farms, using yield and efficiency as evaluation tools. The specific aim was to analyze the behavior of yield and efficiency in five diametric classes: 20.0-25.9cm, 26.0-28.9 cm, 29.0-31.9 cm, 32.0-34.9 cm and 35.0-41.0 cm. According to the results obtained, the chainsaw log breakdown had a satisfactory operational behavior, with average yield and efficiency of 58,5% and 0.55m³/operator/h, respectively. However, the feasibility of such technique is restricted to small scale use. Diametric class 3 (29.0-31.9 cm presented the best yield (66.6% because it was the one that presented the best adequacy between log diameter and products. It is suggested for further researches the development of specific sawing diagrams per diametric class, in addition to the reduction of class ranges, aiming the yield increase. The average breakdown time spent per log was the same for classes 3, 4 and 5, resulting that the latter presented the best efficiency, 0.82 m³/operator/h.

  14. EFEITO DO DESBASTE NAS RACHADURAS DE TOPO DAS TORAS DE Eucalyptus grandis W. Hill ex Maiden

    Directory of Open Access Journals (Sweden)

    Rômulo Trevisan

    2014-01-01

    Full Text Available The study was undertaken to evaluate the effect of thinning in the end splits of the logs of the Eucalyptus grandis at 18 years of age. There was analyzed a thinning experiment, located in the northern coast of Rio Grande do Sul State, Brazil, installed in randomized blocks with four repetitions. The seven treatments were defined according to the number of thinnings applied and ranged from zero to six interventions. The trees were selected, in each treatment, on the basis of the Assmann’s dominant diameter and the central diameter of tree, being referred to as dominant and central trees, respectively. After cutting down the selected the logs were located between the DBH (diameter at breast height and 25% positions, 25% and 50%; 50% and 75%; 75% and 100% of the commercial height, for the end split study. The results indicate that the average end splits of the logs in the dominant and central trees was higher at the second log sampled, suggesting that sectioning should be carried in conjunction with techniques that minimize the growth stress, especially when this is performed near the half of the commercial height. The thinning induced variation in the logs end split, both for dominant and central trees; however, there was no clear positive or negative trend, leading to the conclusion that changes in growth rate did not af fect these parameters uniformly.

  15. Química e colorimetria da madeira de Eucalyptus grandis W. Mill ex Maiden termorretificada

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    Antônio José Vinha Zanuncio

    2014-08-01

    Full Text Available A termorretificação visa melhorar as características para agregar valor à madeira através da aplicação de calor. O objetivo foi avaliar as alterações químicas e colorimétricas da madeira termorretificada de Eucalyptus grandis submetida a 170, 200 e 230 ºC por 3, 5 e 7 h. Os teores de extrativos, lignina, holocelulose, composição elementar e os parâmetros colorimétricos foram determinados. Os teores de extrativos aumentaram nos tratamentos a 170 °C e diminuíram a partir de 200 °C, enquanto o teor de lignina total aumentou e o de holocelulose diminuiu a partir de 200 °C. O teor de carbono aumentou e o de oxigênio diminuiu a 230 °C por 5 e 7 h. A termorretificação reduziu a claridade, matriz vermelho (a* e matriz amarelo (b* da madeira de eucalipto em todos os tratamentos.

  16. RELATIONSHIP BETWEEN THE ANATOMY AND DRYING IN Eucalyptus grandis X Eucalyptus urophylla WOOD

    Directory of Open Access Journals (Sweden)

    Antônio José Vinha Zanuncio

    Full Text Available ABSTRACT Drying is an important step to using wood and anatomical characteristics influence this process. Thus, the objective of this work was to evaluate the relationship between anatomy and wood drying. Samples with 2 x 2 x 4 cm were obtained from eight Eucalyptus urophylla x Eucalyptus grandis clones at 1.3 m height to evaluate the anatomy and drying in climate chamber during 15 days. Cell wall fraction was established as the parameter that best correlated with drying, the Pearson correlation coefficient between this parameter and moisture was -0.8986, -0.6580, 0.9216, 0.8743, 0.7131 and 0.8727 for saturated wood, and after 1, 2.5, 5, 10 and 15 days of drying, respectively. The frequency and vessel size, as well as the height and width of the rays showed low relation with wood drying. Wood anatomy, mainly the cell wall fraction, influences moisture losses and should be considered in wood drying programs.

  17. Effect of fertilization on cell size in wood of Eucalyptus grandis Hill Ex Maiden

    Directory of Open Access Journals (Sweden)

    Luiz Santini Junior

    2010-12-01

    Full Text Available The use of fertilization in forest stands results in yield gains, yet little attention has been directed to its potential effects on the quality of wood produced. Information is scarce about the effect of fertilization on anatomical structures of older Eucalyptus wood. This work aims to study the effect of fertilization on tissue cell size of wood from a Eucalyptus grandis stand at age 21 years, the management system of which is based on selective thinning and fertilizer application at the start of the thinning season. Factors to consider include: presence or absence of fertilizers, two log positions and five radial (pith to bark positions. Results led to the conclusion that fertilization significantly influenced only vessel frequency. Vessel elements length was influenced by tree height. Fiber length, fiber diameter, fiber wall thickness, vessel elements length, vessel diameter and vessel frequency were influenced by the radial position of the sample in relation to the log. A positive correlation was observed between fiber length, fiber diameter, fiber wall thickness, vessel elements length, vessel diameter, ray width and radial position, while a negative correlation was observed between ray frequency and radial position.

  18. Características de vigas laminadas coladas confeccionadas com madeira de teca (Tectona grandis

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    Full Text Available O objetivo desta pesquisa foi avaliar a influência do adesivo, das gramaturas e dos tempos de prensagem nas propriedades mecânicas de vigas laminadas coladas, confeccionadas com madeira de teca (Tectona grandis. As vigas foram produzidas com cinco lamelas, sendo duas da classe de módulo de elasticidade dinâmico, classe 1 - MOEd C1 nas faces, e três da classe 2 - MOEd C2 no miolo. Para a colagem, utilizou-se o adesivo à base de resorcina-fenol-formaldeído (RFF, com gramaturas de 350 e 450 g.m- 2, e tempos de prensagem de 5 e 8 horas. Para o adesivo à base de emulsão polimérica de isocianato (EPI, a colagem foi realizada com gramaturas de 180 e 220 g.m- 2, e tempos de prensagem de 3 e 4 horas. Os resultados das propriedades mecânicas das vigas indicaram que não houve uma relação bem definida dos efeitos das variáveis de estudo nas propriedades de flexão estática. As vigas coladas com o adesivo RFF tiveram maior resistência da linha de cola em comparação às vigas coladas com o adesivo EPI.

  19. Cellulose nanocrystal from pomelo (C. Grandis osbeck) albedo: Chemical, morphology and crystallinity evaluation

    International Nuclear Information System (INIS)

    Zain, Nor Fazelin Mat; Yusop, Salma Mohamad; Ahmad, Ishak

    2013-01-01

    Citrus peel is one of the under-utilized waste materials that have potential in producing a valuable fibre, which are cellulose and cellulose nanocrystal. Cellulose was first isolated from pomelo (C. Grandis Osbeck) albedo by combination of alkali treatment and bleaching process, followed by acid hydrolysis (65% H 2 SO 4 , 45 °C, 45min) to produce cellulose nanocrystal. The crystalline, structural, morphological and chemical properties of both materials were studied. Result reveals the crystallinity index obtained from X-ray diffraction for cellulose nanocrystal was found higher than extracted cellulose with the value of 60.27% and 57.47%, respectively. Fourier transform infrared showed that the chemical treatments removed most of the hemicellulose and lignin from the pomelo albedo fibre. This has been confirmed further by SEM and TEM for their morphological studies. These results showed that cellulose and cellulose nanocrystal were successfully obtained from pomelo albedo and might be potentially used in producing functional fibres for food application

  20. Anthraquinone effect on kraft cooking curve for the wood of Eucalyptus grandis x Eucalyptus urophylla hybrid

    Directory of Open Access Journals (Sweden)

    Magnos Alan Vivian

    2017-12-01

    Full Text Available This study aimed to evaluate the effects of anthraquinone addition in the cooking curve, with different loads of reagents, through the kraft pulping process, of Eucalyptus grandis x Eucalyptus urophylla wood, to infer about the process parameters. The wood was transformed into chips and classified to conduct the kraft cooking curves. The applied loads were from 10% to 24% of active alkali, with an anthraquinone charge of 0.05% (on dry wood. We evaluated raw and screened yield, reject content, kappa number (delignification level and residual active alkali. We observed that the addition of anthraquinone did not affect the process raw yield, but increased the screened yield by reducing reject content, especially for low loads of active alkali (10% and 12%. Another effect observed was the reduction of the kappa number with the addition of anthraquinone, which indicates the increase of the wood delignification rate. This is reinforced by the lower residual active alkali, which implies greater use of reagents in the process.

  1. Applying Sewage Sludge to Eucalyptus grandis Plantations: Effects on Biomass Production and Nutrient Cycling through Litterfall

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Müller da Silva

    2011-01-01

    Full Text Available In most Brazilian cities sewage sludge is dumped into sanitary landfills, even though its use in forest plantations as a fertilizer and soil conditioner might be an interesting option. Sewage sludge applications might reduce the amounts of mineral fertilizers needed to sustain the productivity on infertile tropical soils. However, sewage sludge must be applied with care to crops to avoid soil and water pollution. The aim of our study was to assess the effects of dry and wet sewage sludges on the growth and nutrient cycling of Eucalyptus grandis plantations established on the most common soil type for Brazilian eucalypt plantations. Biomass production and nutrient cycling were studied over a 36-month period in a complete randomized block design. Four experimental treatments were compared: wet sewage sludge, dry sludge, mineral fertilizer, and no fertilizer applications. The two types of sludges as well as mineral fertilizer increased significantly the biomass of Eucalyptus trees. Wood biomass productions 36 months after planting were similar in the sewage sludge and mineral fertilization treatments (about 80 tons ha−1 and 86% higher than in the control treatment. Sewage sludge application also affected positively leaf litter production and significantly increased nutrient transfer among the components of the ecosystem.

  2. STORAGE TIME EFFECT ON MINI-CUTTINGS ROOTING IN Tectona grandis LINN F. CLONES

    Directory of Open Access Journals (Sweden)

    Yorleny Badilla

    2017-08-01

    Full Text Available ABSTRACT The study aimed to evaluate the influence of storage length on Tectona grandis mini-cuttings survival and rooting. A factorial arrangement (4 x 7 was utilized, based on four clones (Carapá, Ipê, GU5 and TB7 and seven time intervals from mini-cuttings harvesting until final sowing (0, 1, 2, 4, 8, 12 and 16 hours. A randomized block design with three replicates and 16 mini-cuttings per experimental unit was utilized. Survival and rooting rates were evaluated after greenhouse culture (30 days after sowing and after shadow house culture (40 days after sowing; as well as height, collar diameter, aerial and root biomass 55 days after sowing. No significant differences were observed in survival and rooting rates among time intervals in teak mini-cuttings preparation from these four clones. However differences among clones were registered for rooting rate, suggesting a genotypic effect. Survival and rooting rates were very high after greenhouse culture (93% and 90% respectively, as well as survival after culture in a shadow-house (88%.

  3. The GC/MS Analysis of Volatile Components Extracted by Different Methods from Exocarpium Citri Grandis

    Directory of Open Access Journals (Sweden)

    Zhisheng Xie

    2013-01-01

    Full Text Available Volatile components from Exocarpium Citri Grandis (ECG were, respectively, extracted by three methods, that is, steam distillation (SD, headspace solid-phase microextraction (HS-SPME, and solvent extraction (SE. A total of 81 compounds were identified by gas chromatography-mass spectrometry including 77 (SD, 56 (HS-SPME, and 48 (SE compounds, respectively. Despite of the extraction method, terpenes (39.98~57.81% were the main volatile components of ECG, mainly germacrene-D, limonene, 2,6,8,10,14-hexadecapentaene, 2,6,11,15-tetramethyl-, (E,E,E-, and trans-caryophyllene. Comparison was made among the three methods in terms of extraction profile and property. SD relatively gave an entire profile of volatile in ECG by long-time extraction; SE enabled the analysis of low volatility and high molecular weight compounds but lost some volatiles components; HS-SPME generated satisfactory extraction efficiency and gave similar results to those of SD at analytical level when consuming less sample amount, shorter extraction time, and simpler procedure. Although SD and SE were treated as traditionally preparative extractive techniques for volatiles in both small batches and large scale, HS-SPME coupled with GC/MS could be useful and appropriative for the rapid extraction and qualitative analysis of volatile components from medicinal plants at analytical level.

  4. Genetic control of Eucalyptus urophylla and E. grandis resistance to canker caused by Chrysoporthe cubensis.

    Science.gov (United States)

    da Silva Guimarães, Lúcio Mauro; de Resende, Marcos Deon Vilela; Lau, Douglas; Rosse, Leonardo Novaes; Alves, Alexandre Alonso; Alfenas, Acelino Couto

    2010-07-01

    Chrysophorte cubensis induced canker occurs in nearly all tropical and subtropical regions where eucalypts are planted, causing losses in both wood quality and volume productivity, especially so in the warmer and more humid regions of Brazil. The wide inter and intra-specific genetic variability of resistance to canker among Eucalyptus species facilitates the selection of resistant plants. In this study, we evaluated resistance to this pathogen in five Eucalyptus grandis (G) and 15 E. urophylla (U) trees, as well as in 495 individuals from 27 progenies derived from crosses between the trees. In the field, six-months-old test seedlings were inoculated with C. cubensis. Lesion length in the xylem and bark was measured eight months later. The results demonstrated that xylem lesions could preferentially be used for the selection of resistant clones. Eight trees (7 U and 1 G) were susceptible, and the remainder (8 U and 4 G) resistant. Individual narrow and broad sense heritability estimates were 17 and 81%, respectively, thereby suggesting that canker resistance is quantitative and highly dependent on dominance and epistasis.

  5. Effects of steaming treatment on crystallinity and glass transition temperature of Eucalyptuses grandis × E. urophylla

    Science.gov (United States)

    Kong, Lulu; Zhao, Zijian; He, Zhengbin; Yi, Songlin

    To investigate the effects of steaming treatment on crystallinity and glass transition temperature, samples of Eucalyptuses grandis × E. urophylla with moisture content of 50%, 70%, and 90% were steamed in saturated steam at 100 °C for 2, 4, 6, and 8 h. The degree of crystallinity (CrI) and glass transition temperature (Tg) were measured via X-ray diffraction and dynamic mechanical analysis, respectively. Results revealed a crystallinity degree of Eucalyptus of 29.9%-34.2%, and a glass transition temperature of 80-94 °C with moisture contents of steamed samples of 20%. Furthermore, steaming was revealed to have an obvious effect on crystallization and glass transition. Values of CrI and Tg showed similar changing characteristics: increasing initially, followed by a decrease with increasing steaming time, reaching a maximum at 2 h. Water within the wood seemed to promote crystallization and glass transition during steaming. All steamed samples tested in this study reached glass transition temperature after 50 min of steaming, and the residual growth stress was released.

  6. Métodos de captura para formigas em pré-plantio de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Jardel Boscardin

    Full Text Available O presente trabalho objetivou avaliar três métodos de coleta de formigas em uma área de pré-plantio de Eucalyptus grandis em Santa Maria-RS. Foram realizadas amostragens de março a agosto de 2011, utilizando-se armadilha de solo (AS, isca atrativa à base de patê de fígado de frango (IA e extração por Funil de Berlese (FB, sendo seis repetições por tratamento em cada data de coleta. Os tratamentos constituíram-se da aplicação de glifosato, tendo sido realizados controles das plantas infestantes: na linha e na entrelinha de plantio (T1; em faixa de um metro paralela à linha de plantio e de um metro na parte central da entrelinha, sem controle (T2; em faixa de meio metro paralela à linha de plantio (T3, e a testemunha, sem controle de plantas infestantes (T4. A partir dos resultados obtidos, os métodos demonstraram ser complementares entre si, sugerindo a utilização de mais de um método na captura de formigas.

  7. Volatile constituents of redblush grapefruit (Citrus paradisi) and pummelo (Citrus grandis) peel essential oils from Kenya.

    Science.gov (United States)

    Njoroge, Simon Muhoho; Koaze, Hiroshi; Karanja, Paul Nyota; Sawamura, Masayoshi

    2005-12-14

    The volatile constituents of cold-pressed peel essential oils of redblush grapefruit (Citrus paradisi Macfadyen forma Redblush) and pummelo (Citrus grandis Osbeck) from the same locality in Kenya were determined by GC and GC-MS. A total of 67 and 52 compounds, amounting to 97.9 and 98.8% of the two oils, respectively, were identified. Monoterpene hydrocarbons constituted 93.3 and 97.5% in the oils, respectively, with limonene (91.1 and 94.8%), alpha-terpinene (1.3 and 1.8%), and alpha-pinene (0.5%) as the main compounds. Sesquiterpene hydrocarbons constituted 0.4% in each oil. The notable compounds were beta-caryophyllene, alpha-cubebene, and (E,E)-alpha-farnesene. Oxygenated compounds constituted 4.2 and 2.0% of the redblush grapefruit and pummelo oils, respectively, out of which carbonyl compounds (2.0 and 1.3%), alcohols (1.4 and 0.3%), and esters (0.7 and 0.4%) were the major groups. Heptyl acetate, octanal, decanal, citronellal, and (Z)-carvone were the main constituents (0.1-0.5%). Perillene, (E)-carveol, and perillyl acetate occurred in the redblush grapefruit but were absent from the pummelo oil. Nootkatone, alpha- and beta-sinensal, methyl-N-methylanthranilate, and (Z,E)-farnesol were prominent in both oils.

  8. Effect of controlled irrigation on physiological and biometric characteristics in teak (Tectona grandis seedlings

    Directory of Open Access Journals (Sweden)

    Sneha C.

    2012-08-01

    Full Text Available Effect of controlled irrigation in physiological and biometric characteristics in teak seedlings is monitored at Forestry College in Kerala, India. Six month old seedlings of Teak (Tectona grandis L. f. were raised in polybags. Irrigation was done once in a week. Daily evapotranspiration was calculated and treatments IW/ET=1, IW/ET=0.6, IW/ET=0.3 were irrigated with 100, 60 and 30 per cent of cumulative evapotranspiration. A control without irrigation (IW/ET=0 was also maintained. Physiological as well as biometric observations were carried out at regular intervals. Canopy air temperature difference (CATD was measured using a hand held infrared thermometer and it was observed that seedlings from well watered treatments (IW/ET=1 and 0.6 showed negative CATD whereas treatments IW/ET=0.3 and IW/ET=0 showed positive CATD throughout the growing period. Seedlings in the treatment IW/ET= 0 and 0.3 showed a significant reduction in relative chlorophyll content, seedling height, collar diameter, number of leaves, total dry weight and relative growth rate whereas in IW/ET=1 and 0.6 these parameters were higher and there was no significant difference observed between these two well watered treatment. In the case of root shoot length ratio and root shoot biomass ratio IW/ET=0 was superior over other three treatments.

  9. Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce (Picea abies).

    Science.gov (United States)

    Phillips, Michael A; Walter, Michael H; Ralph, Steven G; Dabrowska, Paulina; Luck, Katrin; Urós, Eva Maria; Boland, Wilhelm; Strack, Dieter; Rodríguez-Concepción, Manuel; Bohlmann, Jörg; Gershenzon, Jonathan

    2007-10-01

    Conifers produce terpenoid-based oleoresins as constitutive and inducible defenses against herbivores and pathogens. Much information is available about the genes and enzymes of the late steps of oleoresin terpenoid biosynthesis in conifers, but almost nothing is known about the early steps which proceed via the methylerythritol phosphate (MEP) pathway. Here we report the cDNA cloning and functional identification of three Norway spruce (Picea abies) genes encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS), which catalyzes the first step of the MEP pathway, and their differential expression in the stems of young saplings. Among them are representatives of both types of plant DXS genes. A single type I DXS gene is constitutively expressed in bark tissue and not affected by wounding or fungal application. In contrast, two distinct type II DXS genes, PaDXS2A and PaDXS2B, showed increased transcript abundance after these treatments as did two other genes of the MEP pathway tested, 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and 4-hydroxyl 3-methylbutenyl diphosphate reductase (HDR). We also measured gene expression in a Norway spruce cell suspension culture system that, like intact trees, accumulates monoterpenes after treatment with methyl jasmonate. These cell cultures were characterized by an up-regulation of monoterpene synthase gene transcripts and enzyme activity after elicitor treatment, as well as induced formation of octadecanoids, including jasmonic acid and 12-oxophytodienoic acid. Among the Type II DXS genes in cell cultures, PaDXS2A was induced by treatment with chitosan, methyl salicylate, and Ceratocystis polonica (a bark beetle-associated, blue-staining fungal pathogen of Norway spruce). However, PaDXS2B was induced by treatment with methyl jasmonate and chitosan, but was not affected by methyl salicylate or C. polonica. Our results suggest distinct functions of the three DXS genes in primary and defensive terpenoid metabolism in Norway

  10. Expression analysis of a ''Cucurbita'' cDNA encoding endonuclease

    International Nuclear Information System (INIS)

    Szopa, J.

    1995-01-01

    The nuclear matrices of plant cell nuclei display intrinsic nuclease activity which consists in nicking supercoiled DNA. A cDNA encoding a 32 kDa endonuclease has been cloned and sequenced. The nucleotide and deduced amino-acid sequences show high homology to known 14-3-3-protein sequences from other sources. The amino-acid sequence shows agreement with consensus sequences for potential phosphorylation by protein kinase A and C and for calcium, lipid and membrane-binding sites. The nucleotide-binding site is also present within the conserved part of the sequence. By Northern blot analysis, the differential expression of the corresponding mRNA was detected; it was the strongest in sink tissues. The endonuclease activity found on DNA-polyacrylamide gel electrophoresis coincided with mRNA content and was the highest in tuber. (author). 22 refs, 6 figs

  11. Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

    International Nuclear Information System (INIS)

    Han, J.H.; Stratowa, C.; Rutter, W.J.

    1987-01-01

    The authors have cloned a full-length putative rat pancreatic lysophospholipase cDNA by an improved mRNA isolation method and cDNA cloning strategy using [ 32 P]-labelled nucleotides. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long and relatively rare cDNA clone containing the entire sequence from the cap site at the 5' end to the poly(A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid composition, and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase

  12. Non-compressible ABIs are associated with an increased risk of major amputation and major adverse cardiovascular events in patients with critical limb ischemia.

    Science.gov (United States)

    Singh, Gagan D; Armstrong, Ehrin J; Waldo, Stephen W; Alvandi, Bejan; Brinza, Ellen; Hildebrand, Justin; Amsterdam, Ezra A; Humphries, Misty D; Laird, John R

    2017-06-01

    Ankle-brachial indices (ABIs) are important for the assessment of disease burden among patients with peripheral artery disease. Although low values have been associated with adverse clinical outcomes, the association between non-compressible ABI (ncABI) and clinical outcome has not been evaluated among patients with critical limb ischemia (CLI). The present study sought to compare the clinical characteristics, angiographic findings and clinical outcomes of those with compressible (cABI) and ncABI among patients with CLI. Consecutive patients undergoing endovascular evaluation for CLI between 2006 and 2013 were included in a single center cohort. Major adverse cardiovascular events (MACE) were then compared between the two groups. Among 284 patients with CLI, 68 (24%) had ncABIs. These patients were more likely to have coronary artery disease ( p=0.003), diabetes ( pcABI. Rates of infrapopliteal disease were similar between the two groups ( p=0.10), though patients with ncABI had lower rates of iliac ( p=0.004) or femoropopliteal stenosis ( p=0.003). Infrapopliteal vessels had smaller diameters ( p=0.01) with longer lesions ( p=0.05) among patients with ncABIs. After 3 years of follow-up, ncABIs were associated with increased rates of mortality (HR 1.75, 95% CI: 1.12-2.78), MACE (HR 2.04, 95% CI: 1.35-3.03) and major amputation (HR 1.96, 95% CI: 1.11-3.45) when compared to patients with cABIs. In conclusion, ncABIs are associated with higher rates of mortality and adverse events among those undergoing endovascular therapy for CLI.

  13. First occurrence of Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) preying on defoliating caterpillars of oil palm in the state of Para, Brazil; Primeira ocorrencia de Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) predando lagartas desfolhadoras do dendezeiro no estado do Para, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Rafael C.; Lemos, Walkymario P.; Muller, Antonio A. [EMBRAPA Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia], e-mail: rafaufra@yahoo.com.br, e-mail: wplemos@cpatu.embrapa.br; Muller, Antonio A. [Embrapa Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia; Bernardino, Aline S.; Buecke, Joel [Grupo Agropalma S/A., Tailandia, PA (Brazil)

    2010-01-15

    The oil palm Elaeis guineensis is usually attacked by pests, particularly, defoliating caterpillars. Between 2004 and 2006 a stinkbug predator (Asopinae) was registered preying on caterpillars of Brassolis sophorae L., Opsiphanes invirae Hubner (Lepidoptera: Nymphalidae) and Sibine spp. (Lepidoptera: Limacodidae), reducing their populations in commercial oil palm plantations in the State of Para, Brazil. Specimens of the natural enemy were collected, mounted, and identified as Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae), corresponding to the first report of the occurrence of this stinkbug attacking defoliating caterpillars of oil palm in Brazil. (author)

  14. Construction and analysis of a cDNA library from yellow-fruit ginseng

    African Journals Online (AJOL)

    The total RNA was isolated from yellow-fruit ginseng (Panax ginseng C.A. Meyer) leaf tissue. A cDNA library of panax ginseng leaves was constructed by using pDNR-LIB vector according to the SMART cDNA library construction kit protocol. We obtained 378 high quality sequences (GenBank accession number: ...

  15. cDNA table - RPD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...ile URL: ftp://ftp.biosciencedbc.jp/archive/rpd/LATEST/rpd_cdna.zip File size: 15 KB Simple search URL http:...age About This Database Database Description Download License Update History of This Database Site Policy | Contact Us cDNA table - RPD | LSDB Archive ...

  16. [Construction of cDNA library of Magnaporthe grisea with magnetic bead].

    Science.gov (United States)

    Feng, Xu; Xiaoli, Wu; Dewen, Qiu

    2008-06-01

    We constructed cDNA library of Magnaporthe grisea. The good quality cDNA library could facilitate finding proteinaceous elicitors of M. grisea, and elucidating the mechanisms of the M. grisea--rice interaction. The Oligo(dT) combined with the magnetic bead was used to extract mRNA from total RNA of Magnaporthe grisea and as primers to synthesize the first-strand cDNA. Terminal transferase introduced PolyA into 3'terminal of the first cDNA strand, then the PolyA was used for amplifying the second-strand cDNA. Restriction enzyme and adapter were avoided in this research, which could solve technical limitation of the traditional method. Because all reactions were done in one centrifuge tube, this process could reduce the risk of cDNA loss and cross-contamination. The primers designed in this research could clone the amplified cDNAs into expression vector in a desirable orientation. The cDNA library constructed had a high titer of 8.9 x 10(6) cfu/mL, and contained a total clones of 8.9 x 10(7) cfu, with an average inserts size of about 1380 bp. Constructing cDNA library with magnetic bead was a highly efficient method using only small amount of experimental materials within a short period.

  17. Development of a simple and powerful method, cDNA AFLP-SSPAG ...

    African Journals Online (AJOL)

    Differential cDNAs were easily obtained from silver stained cDNA-AFLP separated on polyacylamide gels. The cDNA was then reamplified, cloned and fragments were sequenced. Sequenced clones were used as probes in northern dot blot analyses and library screening. Full-length cDNA was cloned from a library ...

  18. Genomic and cDNA cloning of a novel mouse lipoxygenase gene

    NARCIS (Netherlands)

    Willems van Dijk, K.; Steketee, K.; Havekes, L.; Frants, R.; Hofker, M.

    1995-01-01

    A novel 12- and 15-lipoxygenase related gene was isolated from a mouse strain 129 genomic phage library in a screen with a human 15-lipoxygenase cDNA probe. The complete genomic sequence revealed 14 exons and 13 introns covering 7.3 kb of DNA. The splice junctions were verified from the cDNA

  19. Construction of full-length cDNA library of white flower Salvia ...

    African Journals Online (AJOL)

    In order to screen and isolate secondary metabolite biosynthesis related gene, we construct a cDNA library of white flower Salvia miltiorrhiza bge. f.alba. High quality of total RNA was successfully isolated from roots of white flower S. miltiorrhiza using modified CTAB method. Double strand cDNA was cloned into pDNR-LIB ...

  20. Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA

    International Nuclear Information System (INIS)

    Permutt, M.A.; Koranyi, L.; Keller, K.; Lacy, P.E.; Scharp, D.W.; Mueckler, M.

    1989-01-01

    Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-K m , low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. The authors isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus

  1. Cloning and sequence analysis of H. contortus HC58cDNA gene ...

    African Journals Online (AJOL)

    Phylogenetic analysis revealed close evolutionary proximity of the protein sequence to counterpart sequences in the cathepsin B like proteases, suggesting that HC58cDNA was a member of the papain family. Keywords:Haemonchus contortus, HC58cDNA, cathepsin B like protease, papain family. Kenya Veterinarian Vol.

  2. [A novel vector for construction of a cDNA library].

    Science.gov (United States)

    Fedchenko, V I; Kaloshin, A A; Medvedev, A E

    2010-01-01

    A new original vector pEM-(dT)40(f+) has been prepared. It can be used for cDNA library construction from polyadenylated mRNA, isolated from various sources. The pGEM-(dT)40f(+) is initially transformed into single stranded and then into a linear form and its (dT)40 tail at 3'-end is used as the vector-primer for synthesis of the first strand cDNA. The use of a synthetic oligonucleotide complementary to the vector and recombinant DNA results in vector cyclization and synthesis of the second strand cDNA. This approach significantly simplifies cDNA library construction, it does not require PCR reaction (which can induce artifact mutations in cDNA sequences) and restrictase treatment.

  3. Selection of Reference Genes for qRT-PCR Analysis of Gene Expression in Stipa grandis during Environmental Stresses.

    Directory of Open Access Journals (Sweden)

    Dongli Wan

    Full Text Available Stipa grandis P. Smirn. is a dominant plant species in the typical steppe of the Xilingole Plateau of Inner Mongolia. Selection of suitable reference genes for the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR is important for gene expression analysis and research into the molecular mechanisms underlying the stress responses of S. grandis. In the present study, 15 candidate reference genes (EF1 beta, ACT, GAPDH, SamDC, CUL4, CAP, SNF2, SKIP1, SKIP5, SKIP11, UBC2, UBC15, UBC17, UCH, and HERC2 were evaluated for their stability as potential reference genes for qRT-PCR under different stresses. Four algorithms were used: GeNorm, NormFinder, BestKeeper, and RefFinder. The results showed that the most stable reference genes were different under different stress conditions: EF1beta and UBC15 during drought and salt stresses; ACT and GAPDH under heat stress; SKIP5 and UBC17 under cold stress; UBC15 and HERC2 under high pH stress; UBC2 and UBC15 under wounding stress; EF1beta and UBC17 under jasmonic acid treatment; UBC15 and CUL4 under abscisic acid treatment; and HERC2 and UBC17 under salicylic acid treatment. EF1beta and HERC2 were the most suitable genes for the global analysis of all samples. Furthermore, six target genes, SgPOD, SgPAL, SgLEA, SgLOX, SgHSP90 and SgPR1, were selected to validate the most and least stable reference genes under different treatments. Our results provide guidelines for reference gene selection for more accurate qRT-PCR quantification and will promote studies of gene expression in S. grandis subjected to environmental stress.

  4. Blood Sugar Lowering Effect of Coccinia grandis (L. J. Voigt: Path for a New Drug for Diabetes Mellitus

    Directory of Open Access Journals (Sweden)

    M. A. A. K. Munasinghe

    2011-01-01

    Full Text Available Background. Role of herbs in the management and control of diabetes has emerged fast over the years. We assessed the efficacy of Coccinia grandis (locally known as Ken, Kovakka leaves as a hypoglycemic agent. Methods. Double-blind phase I clinical trial was conducted at the general hospital and a private hospital in Matara in August 2009. All the participants were given a common meal for dinner, and they maintained a 10-hour fasting period. Sixty-one healthy volunteers were given a meal containing 20 g of leaves of Coccinia grandis which was mixed with a measured amount of scraped coconut and table salt for breakfast, and other 61 were given the placebo meal which also contained scraped coconut and salt. Glucose tolerance test was performed blindly for the two groups. Mixed factorial design analysis of variance and student's t-test were applied. Results. Overall blood sugar levels of the experimental group were also significantly lower than those of the control group (F(1,117 5.56, <0.05. Increase in the blood sugar levels from fasting to one hour (F(1,117 6.77, <0.05 and two hours (F(1,117 5.28, <0.05 postprandially was statistically significant for participants who were in the control group than those of in the experimental group. The mean difference of postprandial blood sugar levels (mg/dL after one hour (20.2, 95% confidence interval, 4.81 to 35.5 and two hours (11.46, 95% confidence interval; 1.03 to 21.9 was statistically significant between the two groups. Conclusions. Coccinia grandis has a blood sugar lowering effect. However further studies are needed to validate our findings.

  5. Effects of an inducible aiiA gene on disease resistance in Eucalyptus urophylla × Eucalyptus grandis.

    Science.gov (United States)

    Ouyang, L J; Li, L M

    2016-08-01

    N-acyl-homoserine lactones (AHLs) are metabolites of mostly gram-negative bacteria and are critical signaling molecules in bacterial quorum-sensing systems. At threshold concentrations, AHLs can activate the expression of pathogenic genes and induce diseases. Therefore, reducing AHL concentrations is a key point of disease control in plants. AHL-lactonase, which is expressed by aiiA, is widespread in Bacillus sp and can hydrolyze AHLs. In the present study, we cloned aiiA from Bacillus subtilis by PCR. A plant expression vector of aiiA was constructed and name Pcam-PPP3-aiiA, in which expression of aiiA was controlled by the pathogen-inducible plant promoter PPP3. The recombinant plasmid was transferred into Eucalyptus × urophylla × E. grandis by an Agrobacterium-mediated transformation. PCR and Southern blotting showed that aiiA was successfully integrated into the E. urophylla × E. grandis genome and its expression was induced by Ralstonia solanacearum 12 h after inoculation, as shown by reverse transcription-PCR. The transcription efficacy of aiiA increased 43.88-, 30.65-, and 18.95-fold after inoculation with R. solanacearum, Erwinia carotovora ssp. zeae (Sabet) and Cylindrocladium quinqueseptatum, respectively as shown by RT-real-time PCR. Transgenic E.urophylla × E.grandis expressing the AIIA protein exhibited significantly enhanced disease resistance compared to non-transgenic plants by delaying the onset of wilting and reducing the disease index.

  6. Length variation in the internal transcribed spacers of ribosomal DNA in Picea abies and related species.

    Science.gov (United States)

    Karvonen, P; Szmidt, A E; Savolainen, O

    1994-12-01

    The structure and variation of nuclear ribosomal DNA (rDNA) units of Picea abies, (L.) Karst. was studied by restriction mapping and Southern hybridization. Conspicuous length variation was found in the internal transcribed spacer (ITS) region of P. abies, although the length of this region is highly conserved both within and among most of the plant species. Two types of ITS variants (A and B), displaying a size difference of 0.5 kb in the ITS2 region, were present within individuals of P. abies from Sweden, Central Europe and Siberia. A preliminary survey of 14 additional Eurasian and North American species of Picea suggested that length variation in the ITS region is widespread in this genus. Alltogether three length variants (A, B and C) were identified. Within individuals of eight Picea species, two length variants were present within the genome (combinations of A and B variants in P. glehnii, P. maximowiczii, P. omorika, P. polita and P. sitchensis and variants B and C in P. jezoensis, P. likiangensis and P. spinulosa). Within individuals from five species, however only one rDNA variant was present in their genome (variant A in P. aurantiaca, P. engelmannii, P. glauca, P. koraiensis and P. koyamai; variant B in P. bicolor). The ITS length variation will be useful as a molecular marker in evolutionary studies of the Picea species complex, whose phylogeny is controversial. The presence of intraindividual variation in, and shared polymorphism of the, ITS length variants raises questions about the occurrence of interspecific hybridization during the evolutionary history of Picea.

  7. Early testing of adaptedness to temperature and water availability in Pinus sylvestris and Picea abies

    International Nuclear Information System (INIS)

    Sonesson, Johan

    2000-01-01

    Long-term climate changes have been evident in the past. In the future an increase in the rate of climate change is predicted owing to man-made emissions. Studies of adaptedness to different climatic conditions are of great importance for the design of appropriate breeding and gene conservation programmes. This thesis presents studies of adaptedness to temperature and water availability in Pinus sylvestris and Picea abies and explores the possibilities of utilising differences in adaptedness to obtain juvenile-mature (J-M) correlations strong enough for efficient early testing. Offspring from clones in two Swedish Pinus sylvestris seed orchards and one Picea abies seed orchard were grown in growth chambers for one and two growth periods respectively. Two temperature regimes and two irrigation regimes were applied in a factorial design. Both species expressed high phenotypic plasticity and additive variance for height growth and biomass traits. This implies that these populations should be able to adapt both to short-term and to long-term climate changes. Genotype by environment (GxE) interaction indicated strong differences in adaptedness to temperature and low differences in adaptedness to water availability. Parent rank changes between treatments indicated that climate change could seriously alter the ranking of clones in breeding populations and thus decrease the genetic gain obtained in previous selections. Differences in stability among parents suggested that culling of unstable genotypes could be a way to reduce the negative effects of GxE interaction. Genetic correlations between growth chamber and 14-30 year old field progeny trials with the same parents were mainly weak for both species. The correlations were improved by the drought treatment in the Picea abies experiment suggesting that further development of early testing methods for this species should include treatments with limiting water availability

  8. E3B1/ABI-1 Isoforms Are Down-Regulated in Cancers of Human Gastrointestinal Tract

    Directory of Open Access Journals (Sweden)

    Rafia A. Baba

    2012-01-01

    Full Text Available The expression of E3B1/ABI-1 protein and its role in cancer progression and prognosis are largely unknown in the majority of solid tumors. In this study, we examined the expression pattern of E3B1/ABI-1 protein in histologically confirmed cases of esophageal (squamous cell carcinoma and adenocarcinoma, gastro-esophageal junction, colorectal cancers and corresponding normal tissues freshly resected from a cohort of 135 patients, by Western Blotting and Immunofluorescence Staining. The protein is present in its phosphorylated form in cells and tissues. Depending on the extent of phosphorylation it is either present in hyper-phosphorylated (M. Wt. 72 kDa form or in hypo-phosphorylated form (M. Wt. 68 kDa and 65 kDa. A thorough analysis revealed that expression of E3B1/ABI-1 protein is significantly decreased in esophageal, gastro-esophageal junction and colorectal carcinomas irrespective of age, gender, dietary and smoking habits of the patients. The decrease in expression of E3B1/ABI-1 was consistently observed for all the three isoforms. However, the decrease in the expression of isoforms varied with different forms of cancers. Down-regulation of E3B1/ABI-1 expression in human carcinomas may play a critical role in tumor progression and in determining disease prognosis.

  9. Wood density in commercial plantations of eucalyptus in the Mogi-Guacu region (Sao Paulo). [E. grandis, E. urophylla; E. saligna

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, C.A.; de Freitas, M.; Ferreira, M.

    1979-01-01

    Studies were made on samples of 3-7 yr old Eucalyptus saligna, 3-7 yr old Eucalyptus grandis and 7-8 yr old Eucalyptus urophylla (all of Rio Claro, Brazil, seed source) and 3-5 yr old Eucalyptus saligna and 2-6 yr old Eucalyptus grandis of Coff's Harbour, NSW, seed source. Between-tree variation was high and greater than variation between plantations at the same locality. Annual volume increment was 10-30 steres/ha for Brazilian material, 30-46 steres/ha for NSW material. 11 references.

  10. Comparison of deterministically predicted genetic gains with those realised in a South African Eucalyptus grandis breeding program

    CSIR Research Space (South Africa)

    Verryn, SD

    2009-06-01

    Full Text Available . These assumptions include those of the Hardy-Weinberg population (Hardy 1908; Weinberg 1908), i.e.: • Applies to a large population • Absence of selection • Random mating, i.e. no inbreeding/ selfing The CSIR E.grandis F3 breeding population was in part... al. 2000). It was assumed that the coefficient of relationship was 0.3, on the basis of a study (Verryn 1993) that estimated that there is approximately 20% inbreeding in the open pollinated population (see also Griffin et al. 1987; Griffin...

  11. Eradication of the cotton boll weevil (Anthonomus grandis) in the United States - A successful multi-regional approach

    International Nuclear Information System (INIS)

    Cunningham, Gary L.; Grefenstette, William J.

    2000-01-01

    The cotton boll weevil, Anthonomus grandis Boheman, is believed to have entered the US from Mexico and was first detected in South Texas in 1892. Since that time, the pest has spread throughout most of the nation's cotton-producing areas and has become the industry's number one nemesis. More than US$13 billion in economic losses have occurred since its introduction, with recent annual expenditures of more than US$300 million for control costs alone. Although the weevil has been eradicated from over 4 million acres, its presence in non-programme areas continues to dictate production practices within the mid-south, Texas and Oklahoma

  12. ELi abi ettevõtjale lükkub osaliselt edasi / Silva Männik

    Index Scriptorium Estoniae

    Männik, Silva, 1974-

    2004-01-01

    Eesti ettevõtjatele lubatud Euroopa Liidu tõukefondide raha taotlemine Ettevõtluse Arendamise Sihtasutuses viibib majandusministeeriumis vastuvõtmata määruste tõttu. Diagrammid: Struktuurifondidest saadava abi kõrval peab Eesti liikmemaksu tasuma; EAS plaanis jagada tänavu ligi 800 miljonit krooni struktuurifondide raha. Nimekiri: Põhiosa euroabist läheb töötajate arengusse ja transporti. 5 prioriteeti. Vt. samas: Raha venimine kõige hullem. AS-i E-Arsenal juht Jüri Tümanok rahade viibimisest

  13. Modelling initial mortality of Abies religiosa in a crown fire in Mexico

    OpenAIRE

    Temiño Villota, Salomé; Rodríguez Trejo, Dante A.; Molina Terrén, Domingo; Ryan, Kevin C.

    2016-01-01

    Aim of study: The objectives of this work were to determine which morphological and fire severity variables may help explain the mortality of adult Abies religiosa (Kunth) Schltdl. & Cham., to model the probability of this species after being affected by crown fire, and to obtain more elements to classify the sacred fir in terms of fire resistance. This type of studies are relevant to estimate the impact of crown fires on the climax forests that forms this species.Area of study: The burne...

  14. Effects of irrigation, fertilization and drought on the occurrence of Lophodermium piceae in Picea abies needles

    Energy Technology Data Exchange (ETDEWEB)

    Lehtijaervi, Asko; Barklund, Pia [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Mycology and Pathology

    1999-08-01

    Effects of irrigation, drought and fertilization on the frequency of the fungal endophyte Lophodermium piceae in green needles was assessed in a 30-year-old experimental stand of Picea abies in southern Sweden. Frequencies of needles with L. piceae were lower in irrigation and ammonium sulphate fertilization treatments than in the control. Drought treatment frequencies were similar to the control. Needles were susceptible to colonization for at least 3 years; colonization increased with needle age. The results indicate that the increased availability of water to the root system as well as ammonium sulphate fertilization indirectly delays colonization of needles by L. piceae 21 refs, 3 figs, 2 tabs

  15. The anatomical structure of the common fir (Abies alba Mili. bark. I. Development of bark tissues

    Directory of Open Access Journals (Sweden)

    W. O. Golinowski

    2015-01-01

    Full Text Available Qualitative and quantitative changes in bark tissues of the fir (Abies alba connected with the long-term cambium activity were investigated: 1 the development of secondary phloem elements forming the annual ring in different-aged parts of the stem was studied in detail; 2 the terms of formation of the phloem elements were established as well as the duration of the periods of their differentiation and functioning; 3 observations were made of the changes occurring in nonconducting phloem and primary cortex tissues; 4 the course of development of the periderm was studied.

  16. Preliminary notes on the population structure of Heterobasidion abietinum from Abies pinsapo in Spain

    Directory of Open Access Journals (Sweden)

    N. Luchi

    2009-01-01

    Full Text Available A molecular study using M13 minisatellite amplifi cation was carried out to ascertain the origin of the fungal isolates of Heterobasidion abietinum collected from Abies pinsapo in Andalucia (Spain. Isolates were compared with those from A. alba in neighboring countries. Analysis of molecular variance (AMOVA detected signifi cant differences between H. abietinum isolates from A. pinsapo and isolates from A. alba in France and Italy. Variance within the population from A. pinsapo was lower. The genetic differentiation of the fungus reflects differences in the distribution of the host species such as A. pinsapo and A. alba.

  17. The afterlife of antiquity and modern art: Aby Warburg on Manet

    Directory of Open Access Journals (Sweden)

    Dimitrios Latsis

    2015-12-01

    Full Text Available Aby Warburg’s manuscript on Édouard Manet – unpublished during his lifetime and presented here for the first time in English – constitutes one of his rare, substantial commentaries on nineteenth century art. Using “Le Déjeuner sur l’herbe” as a departure point, Warburg proceeds from his customary meticulous investigation of the central motif’s “visual archeology,” to a larger reflection on the evolution of the representation of nature in art and the image of antiquity that modernity has created for itself.

  18. PHYSICAL AND MECHANICAL PROPERTIES OF LVL PANELS MADE FROM Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Hernando Alfonso Lara Palma

    2011-09-01

    Full Text Available This paper mainly aimed to evaluate the physical and mechanical properties of LVL panels made from Eucalyptus grandis, from reforestation at the region of Sengés, in Paraná state, Brazil. LVL panels were manufactured using 23 veneers (2,4mm thick each one in commercial dimension of 2.500 mm long and 1,200 mm wide. The properties of static bending were analyzed (strength and rigidity in beams of the LVL, in the flatwise and edgewise positions. The properties of compression parallel to grain and shear parallel in the plans L-X and L-Y and density in this LVL panels were also analyzed according to ASTM-D 5456/4761 and ASTM-D 198 codes. The mean values to flatwise bending MOE and MOR were 13114 MPa and 88.76 MPa, respectively, and for edgewise bending MOE and MOR were 15871 MPa and 88.63 MPa, respectively. The density (12% of the LVL panels and of the veneers were 690 kg/m3 and 649 kg/m3. The mean values to parallel compression MOE and MOR were 16856 MPa and 58.05 MPa, respectively. The mean values of the maximum resistance to shear parallel in the plans L-X and L-Y were 5.96 MPa and 591 MPa, respectively. All these values reached partially or they passed the medium limits of reference (normative codes, researches and commercial catalogs established for LVL panels and original solid wood, attesting overall the quality of those panels produced with this wood.

  19. Nutrient concentration age dynamics of teak (Tectona grandis L.f.) plantations in Central America

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez-Moya, J.; Murillo, R.; Portuguez, E.; Fallas, J. L.; Rios, V.; Kottman, F.; Verjans, J. M.; Mata, R.; Alvarado, A.

    2013-05-01

    Aim of study. Appropriate knowledge regarding teak (Tectona grandis L.f.) nutrition is required for a better management of the plantations to attain high productivity and sustainability. This study aims to answer the following questions: How can it be determined if a teak tree suffers a nutrient deficiency before it shows symptoms? Are nutrient concentration decreases in older trees associated with age-related declines in forest productivity? Area of study. Costa Rica and Panama. Material and Methods. Nutrient concentration in different tree tissues (bole, bark, branches and foliage) were measured at different ages using false-time-series in 28 teak plantations Research highlights. Foliar N concentration decreases from 2.28 in year 1 to 1.76% in year 19. Foliar Mg concentration increases from 0.23 in year 1 to 0.34% in year 19. The foliar concentrations of the other nutrients are assumed to be constant with tree age: 1.33% Ca, 0.88% K, 0.16% P, 0.12% S, 130 mg kg{sup -}1 Fe, 43 mg kg{sup -}1 Mn, 11 mg kg{sup -}1 Cu, 32 mg kg{sup -}1 Zn and 20 mg kg{sup -}1 B. The nutrient concentration values showed can be taken as a reference to evaluate the nutritional status of similar teak plantations in the region. The concentrations of K, Mg and N could be associated with declines in teak plantation productivity as the plantation becomes older. Whether age-related changes in nutrient concentrations are a cause or a consequence of age-related declines in productivity is an issue for future research with the aim of achieving higher growth rates throughout the rotation period. (Author) 35 refs.

  20. PRODUCTIVITY AND COSTS OF PROCESSOR WORKING IN STANDS OF Eucalyptus grandis Hill ex Maiden

    Directory of Open Access Journals (Sweden)

    Bernardo Carlos Tarnowski

    2009-09-01

    Full Text Available In the present work a time study was conducted with the objective of adjusting equations to estimate the time of activities, productivity, operational costs and the production of the processor used in a harvest operation of stands of Eucalyptus grandis in plain topography in the state of Bahia, Brazil. The operational cycle of the processor consisted of the time spent to process a tree, and was divided in to stages, which were assessed using the methotodology of single activity times. The sampling unit was the operational cycle of the machine. The statistical analysis was based on regression analysis considering the selection procedure “stepwise”. With the adjusted equations it was possible to estimate the productivity of the machine taking into account the of tree diameter. Considering an operational efficiency of 70 % under the circumstances of the study, the productivity of the processor was 25,8 m3 cc/h, the operational costs 47,90 US$/h and the production costs 1,86 US$/m3 cc. On the basis of the obtained results it can be concluded that the time of tree processing has varied directly according to the diameter increase diameter; the preparation time, contrary to the processing time, only shows a weak correlation with tree diameter; productivity of the processor is directly proportional to tree diameter, when expressed in volume and inversely proportional when expressed in tree number; the costs per cubic meter of wood processed varies inversely with of increased diameter; from the operational costs, fixed costs had the highest proportion followed by the variable costs, administrative costs and costs for manpower; the production costs of the processor decreased exponentially with increasing tree diameter.

  1. Nutrient concentration age dynamics of teak (Tectona grandis L.f. plantations in Central America

    Directory of Open Access Journals (Sweden)

    J. Fernandez Moya

    2013-04-01

    Full Text Available Aim of study. Appropriate knowledge regarding teak (Tectona grandis L.f. nutrition is required for a better management of the plantations to attain high productivity and sustainability. This study aims to answer the following questions: How can it be determined if a teak tree suffers a nutrient deficiency before it shows symptoms? Are nutrient concentration decreases in older trees associated with age-related declines in forest productivity?Area of study. Costa Rica and Panama.Material and Methods. Nutrient concentration in different tree tissues (bole, bark, branches and foliage were measured at different ages using false-time-series in 28 teak plantationsResearch highlights. Foliar N concentration decreases from 2.28 in year 1 to 1.76% in year 19. Foliar Mg concentration increases from 0.23 in year 1 to 0.34% in year 19. The foliar concentrations of the other nutrients are assumed to be constant with tree age: 1.33% Ca, 0.88% K, 0.16% P, 0.12% S, 130 mg kg-1 Fe, 43 mg kg-1 Mn, 11 mg kg-1 Cu, 32 mg kg-1 Zn and 20 mg kg-1 B. The nutrient concentration values showed can be taken as a reference to evaluate the nutritional status of similar teak plantations in the region. The concentrations of K, Mg and N could be associated with declines in teak plantation productivity as the plantation becomes older. Whether age-related changes in nutrient concentrations are a cause or a consequence of age-related declines in productivity is an issue for future research with the aim of achieving higher growth rates throughout the rotation period.Key words: forest nutrition; age-related decline in productivity; nutrient foliar concentration; nutrient bole concentration; resorption.

  2. PRODUÇÃO DE Eucalyptus grandis Hill ex Maiden EM DIFERENTES INTENSIDADES DE DESBASTE

    Directory of Open Access Journals (Sweden)

    Gerson Mainardi

    1998-12-01

    Full Text Available O efeito de diferentes intensidades de desbaste sobre a produção Eucalyptus grandis Hill ex Maiden implantado no ano de 1981, em espaçamento inicial de 3,0 x 2,0 metros, na região de Cachoeira do Sul, Rio Grande do Sul, foi estudado com delineamento de blocos ao acaso, com duas repetições e quatro tratamentos: testemunha, sem desbastes, manutenção de 40%, 50% e 60% da área basal da testemunha, constando-se diferença significativa entre os tratamentos, ao nível de 5% de probabilidade. A produção máxima (756,3 m3cc/ha foi obtida na testemunha, sem desbaste, que não diferiu estatísticamente de 639,6 m3cc/ha, obtido no tratamento com manutenção de 60% da área basal da testemunha, porém diferiu dos tratamentos com manutenção de 50% e 40% da área basal da testemunha. A redução da área basal incrementou a concentração das freqüências nas maiores classes de diâmetro, deslocando a distribuição para a direita, porém com perdas expressivas de produção total e acréscimo significativo no diâmetro. A manutenção de 60% da área basal da testemunha permitiu as menores perdas de produção, cerca de 15,4%, e um ganho de 6,4cm ou 23,9% em incremento diamétrico das árvores remanescentes quando comparado com a testemunha, sem desbaste.

  3. Crioconservación de semillas de teca (Tectona grandis L.F

    Directory of Open Access Journals (Sweden)

    Ana Hine Gómez

    2013-01-01

    Full Text Available La teca (Tectona grandis L.f es un árbol tropical con gran demanda por la alta calidad de su madera y rápido crecimiento. Los programas de mejoramiento genético para esta especie han dado como resultado semillas de mejor calidad y, al mismo tiempo, el incremento en la calidad de las plantaciones. Con el objetivo de conservar la diversidad genética y garantizar la materia prima para los programas de mejoramiento y reproducción futura, las semillas de teca son mantenidas en bancos de semillas convencionales bajo condiciones de temperatura que oscilan entre 4o y –20oC. Sin embargo, hay otras modalidades que pueden emplearse para complementar la conservación de este valioso germoplasma. La crioconservación o almacenamiento en nitrógeno liquido (NL, -196oC presenta importantes ventajas sobre otras técnicas de conservación, como la posibilidad de almacenamiento por tiempo indefinido y en condiciones de alta estabilidad genética. En este estudio se evaluó la sobrevivencia y regeneración de plantas después del congelamiento de las semilas en nitrógeno líquido (NL, con el uso de la técnica de desecación y congelamiento rápido. La metodología fue evaluada tanto en semillas aisladas del endocarpo (semillas como en semillas rodeadas por el endocarpo (semillas con endocarpo, que permite observar tasas de germinación de 84% y 70% respectivamente, a los 28 días en cultivo, después de la descongelación.

  4. Combined Effects of Deepwater Horizon Crude Oil and Environmental Stressors on Fundulus grandis Embryos.

    Science.gov (United States)

    Rodgers, Maria L; Jones, Elizabeth R; Klinkhamer, Christopher; Mahapatra, Cecon T; Serafin, Jennifer; Bosker, Thijs; Perkins, Christopher; Griffitt, Robert J; De Guise, Sylvain; Sepúlveda, Maria S

    2018-04-16

    In this study, we examined how sensitivity to oil changes in combination with environmental stressors in Fundulus grandis embryos. We exposed embryos (< 24 hours post fertilization) to a range of High Energy Water Accommodated Fraction (HEWAF) concentrations (0-50 ppb total polycyclic aromatic hydrocarbons) made from Macondo oil in conjunction with various environmental conditions (temperature: 20, 30°C; salinity: 3, 7, 30 practical salinity units (PSU); DO: 2, 6 mg/L). Endpoints included mortality, hatching rates, and expression of cytochrome p450 1a and 1c (cyp1a, cyp1c) in hatched larvae. There was 100% mortality for all fish under the 2 ppm dissolved oxygen (DO) regimes. For the 6 mg/L DO treatments, mortality and mean lethal time were generally higher in the 30°C treatments versus the 20°C treatments. Oil increased mortality in fish exposed to the highest concentration in the 20-3-6 (°C-PSU-mg/L), 25-7-6, and 30-30-6 conditions. Hatching was driven by environmental conditions, with oil exposure only having a significant impact on hatching in the 25-7-6 and 30-30-6 groups at the highest HEWAF exposure. Expression of cyp1a was upregulated in most treatment groups versus the controls, with cyp1c expression exhibiting a similar pattern. These data suggest interactive effects among temperature, salinity, and PAHs, highlighting a need to further assess the effects of oil exposure under various environmental conditions. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  5. Development and evaluation of teak (Tectona grandis L.f. taper equations in northern Thailand

    Directory of Open Access Journals (Sweden)

    Andrew J. Warner

    2016-09-01

    Full Text Available Taper refers to the general decrease in the regular outline of a solid body from its base to its tip. Taper models are used to estimate the volume and value of wood products from harvesting trees. Teak (Tectona grandis L.f. is highly valued as one of the world's most preferred timbers and a teak taper equation is required to inform optimal harvesting strategies given the limited plantation resource available in Thailand. Teak taper equations were developed and evaluated based on 331 sample trees collected in 2014 from eight plantations in northern Thailand aged from 10 to 46 yr using two taper model formulations—the Kozak variable-exponent taper model and the Goodwin cubic polynomial model comprising hyperbolic and parabolic terms. Variants based on both model types were fitted using nonlinear regression analysis with diameter at breast height, total tree height and height of girth measurement as the independent variables to estimate diameter underbark at the nominated height. Goodness-of-fit and leave-one-out cross validation with lack-of-fit statistical testing combined with extensive graphical analysis of residuals were used to select the best model. A Goodwin model variant (named FIO-teak1 as the first plantation teak taper model known to be published in Thailand provided the best estimates of volume and diameter underbark. A simple case study confirmed that FIO-teak1 in combination with the Farm Forestry Toolbox software package could assist teak plantation managers in decision making associated with optimizing log grade value based on standing tree inventory data.

  6. Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis fruits in Vietnam.

    Directory of Open Access Journals (Sweden)

    Ivana Puglisi

    Full Text Available Two distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis, a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of the rDNA and the cytochrome oxidase subunit 1 (COI as barcode genes, one of the two taxa was provisionally named as Phytophthora sp. prodigiosa, being closely related to but distinct from P. insolita, a species in Phytophthora Clade 9, while the other one, was closely related to but distinct from the Clade 2 species P. meadii and was informally designated as Phytophthora sp. mekongensis. Isolates of P. sp. prodigiosa and P. sp. mekongensis were also obtained from necrotic fibrous roots of Volkamer lemon (C. volkameriana rootstocks grafted with 'King' mandarin (Citrus nobilis and from trees of pomelo, respectively, in other provinces of the Mekong River Delta, indicating a widespread occurrence of both Phytophthora species in this citrus-growing area. Koch's postulates were fulfilled via pathogenicity tests on fruits of various Citrus species, including pomelo, grapefruit (Citrus x paradisi, sweet orange (Citrus x sinensis and bergamot (Citrus x bergamia as well as on the rootstock of 2-year-old trees of pomelo and sweet orange on 'Carrizo' citrange (C. sinensis 'Washington Navel' x Poncirus trifoliata. This is the first report of a Phytophthora species from Clade 2 other than P. citricola and P. citrophthora as causal agent of fruit brown rot of Citrus worldwide and the first report of P. insolita complex in Vietnam. Results indicate that likely Vietnam is still an unexplored reservoir of Phytophthora diversity.

  7. Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam.

    Science.gov (United States)

    Puglisi, Ivana; De Patrizio, Alessandro; Schena, Leonardo; Jung, Thomas; Evoli, Maria; Pane, Antonella; Van Hoa, Nguyen; Van Tri, Mai; Wright, Sandra; Ramstedt, Mauritz; Olsson, Christer; Faedda, Roberto; Magnano di San Lio, Gaetano; Cacciola, Santa Olga

    2017-01-01

    Two distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis), a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of the rDNA and the cytochrome oxidase subunit 1 (COI) as barcode genes, one of the two taxa was provisionally named as Phytophthora sp. prodigiosa, being closely related to but distinct from P. insolita, a species in Phytophthora Clade 9, while the other one, was closely related to but distinct from the Clade 2 species P. meadii and was informally designated as Phytophthora sp. mekongensis. Isolates of P. sp. prodigiosa and P. sp. mekongensis were also obtained from necrotic fibrous roots of Volkamer lemon (C. volkameriana) rootstocks grafted with 'King' mandarin (Citrus nobilis) and from trees of pomelo, respectively, in other provinces of the Mekong River Delta, indicating a widespread occurrence of both Phytophthora species in this citrus-growing area. Koch's postulates were fulfilled via pathogenicity tests on fruits of various Citrus species, including pomelo, grapefruit (Citrus x paradisi), sweet orange (Citrus x sinensis) and bergamot (Citrus x bergamia) as well as on the rootstock of 2-year-old trees of pomelo and sweet orange on 'Carrizo' citrange (C. sinensis 'Washington Navel' x Poncirus trifoliata). This is the first report of a Phytophthora species from Clade 2 other than P. citricola and P. citrophthora as causal agent of fruit brown rot of Citrus worldwide and the first report of P. insolita complex in Vietnam. Results indicate that likely Vietnam is still an unexplored reservoir of Phytophthora diversity.

  8. Construction and characterization of a normalized cDNA library.

    Science.gov (United States)

    Soares, M B; Bonaldo, M F; Jelene, P; Su, L; Lawton, L; Efstratiadis, A

    1994-09-27

    We have developed a simple procedure based on reassociation kinetics that can reduce effectively the high variation in abundance among the clones of a cDNA library that represent individual mRNA species. For this normalization, we used as a model system a library of human infant brain cDNAs that were cloned directionally into a phagemid vector and, thus, could be easily converted into single-stranded circles. After controlled primer extension to synthesize a short complementary strand on each circular template, melting and reannealing of the partial duplexes at relatively low C0t, and hydroxyapatite column chromatography, unreassociated circles were recovered from the flow through fraction and electroporated into bacteria, to propagate a normalized library without a requirement for subcloning steps. An evaluation of the extent of normalization has indicated that, from an extreme range of abundance of 4 orders of magnitude in the original library, the frequency of occurrence of any clone examined in the normalized library was brought within the narrow range of only 1 order of magnitude.

  9. Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma

    OpenAIRE

    Liu, Yong-Bo; Wei, Zhao-Xia; Li, Li; Li, Hang-Sheng; Chen, Hui; Li, Xiao-Wen

    2003-01-01

    AIM: To construct subtracted cDNA libraries of human vascular endothelial cells (VECs) related to gastrocarcinoma using suppression substractive hybridization (SSH) and to analyze cDNA libraries of gastrocarcinoma and VECs in Cancer Gene Anatomy Project (CGAP) database.

  10. Characterization of variable EST SSR markers for Norway spruce (Picea abies L.

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    Spiess Nadine

    2011-10-01

    Full Text Available Abstract Background Norway spruce is widely distributed across Europe and the predominant tree of the Alpine region. Fast growth and the fact that timber can be harvested cost-effectively in relatively young populations define its status as one of the economically most important tree species of Northern Europe. In this study, EST derived simple sequence repeat (SSR markers were developed for the assessment of putative functional diversity in Austrian Norway spruce stands. Results SSR sequences were identified by analyzing 14,022 publicly available EST sequences. Tri-nucleotide repeat motifs were most abundant in the data set followed by penta- and hexa-nucleotide repeats. Specific primer pairs were designed for sixty loci. Among these, 27 displayed polymorphism in a testing population of 16 P. abies individuals sampled across Austria and in an additional screening population of 96 P. abies individuals from two geographically distinct Austrian populations. Allele numbers per locus ranged from two to 17 with observed heterozygosity ranging from 0.075 to 0.99. Conclusions We have characterized variable EST SSR markers for Norway spruce detected in expressed genes. Due to their moderate to high degree of variability in the two tested screening populations, these newly developed SSR markers are well suited for the analysis of stress related functional variation present in Norway spruce populations.

  11. Modelling initial mortality of Abies religiosa in a crown fire in Mexico

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    Salomé Temiño-Villota

    2016-04-01

    Full Text Available Aim of study: The objectives of this work were to determine which morphological and fire severity variables may help explain the mortality of adult Abies religiosa (Kunth Schltdl. & Cham., to model the probability of this species after being affected by crown fire, and to obtain more elements to classify the sacred fir in terms of fire resistance. This type of studies are relevant to estimate the impact of crown fires on the climax forests that forms this species.Area of study: The burned forest was located in the southern Mexico City, borough.Material and methods: Morphological variables and fire severity indicators were collected for 335 Abies religiosa trees burned by a mixed severity fire. Logistic regression was used to analyze data and develop models that best explained tree mortality.Main results: Survival was 26.9%. The models for height (p≤0.0001, diameter at breast height (p=0.0082, crown length (p≤0.0001 and crown base height (p≤0.0001 were significant, with a negative relationship between each one of these variables and probability of mortality. The significant severity variables were lethal scorch height (p≤0.0001 and crown kill (p≤ 0.0001, which have a direct relationship with probability of mortality.Highlights: This species is moderately fire-resistant. Crown kill ≥ 70% markedly increases mortality. Silvicultural activities such as pruning, thinning and fuel management can reduce the risk of crown fires.

  12. Modelling initial mortality of Abies religiosa in a crown fire in Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Temiño-Villota, S.; Rodríguez-Trejo, D.A.; Molina Terrén, D.M.; Ryan, K.

    2016-07-01

    Aim of the study: The objectives of this work were to determine which morphological and fire severity variables may help explain the mortality of adult Abies religiosa (Kunth) Schltdl. & Cham., to model the probability of this species after being affected by crown fire, and to obtain more elements to classify the sacred fir in terms of fire resistance. This type of studies are relevant to estimate the impact of crown fires on the climax forests that forms this species. Area of study: The burned forest was located in the southern Mexico City, borough. Material and methods: Morphological variables and fire severity indicators were collected for 335 Abies religiosa trees burned by a mixed severity fire. Logistic regression was used to analyze data and develop models that best explained tree mortality. Main results: Survival was 26.9%. The models for height (p≤0.0001), diameter at breast height (p=0.0082), crown length (p≤0.0001) and crown base height (p≤0.0001) were significant, with a negative relationship between each one of these variables and probability of mortality. The significant severity variables were lethal scorch height (p≤0.0001) and crown kill (p≤ 0.0001), which have a direct relationship with probability of mortality. Highlights: This species is moderately fire-resistant. Crown kill ≥ 70% markedly increases mortality. Silvicultural activities such as pruning, thinning and fuel management can reduce the risk of crown fires. (Author)

  13. Worm infestations and development of autoimmunity in children - The ABIS study.

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    Johnny Ludvigsson

    Full Text Available Worm infestations influence the immune system and may therefore decrease the risk for autoimmune diseases. The aim of the study was to determine whether children who have developed autoimmune disease were less likely to have had worm infestations in childhood. The ABIS-study is a prospective population-based cohort study of children born in southeast Sweden 1997/99. 17.055 children participated. As of June 2014 116 individuals had developed Type 1 diabetes, 181 celiac disease, and 53 Juvenile Rheumatoid Arthritis. The parents answered questions on worm infestations when the children were 1, 5 and 8 years of age. The ABIS registry was connected to the National Registry of Drug Prescriptions, and national registries for diagnosis of the studied diseases. We found no differences in incidence of worm infestations at 1, 5 or 8 years of age between children who developed autoimmune disease(s or healthy controls. At 8 years in total 20.0% of the general child population had experienced a worm infestation; children who developed Type 1 diabetes, 21,3%, celiac disease 19,5% and JRA 18,8%. There was no difference in prescriptions of drugs for treatment of worm infestations between those who had and who had not developed Type 1 diabetes, celiac disease, Juvenile Rheumatoid Arthritis. We found no associations indicating that worm infestations in childhood does not play a role in the development of autoimmune diseases in Sweden.

  14. The Andalusian Bipolar Family (ABiF) Study: Protocol and sample description.

    Science.gov (United States)

    Guzman-Parra, Jose; Rivas, Fabio; Strohmaier, Jana; Forstner, Andreas; Streit, Fabian; Auburger, Georg; Propping, Peter; Orozco-Diaz, Guillermo; González, Maria José; Gil-Flores, Susana; Cabaleiro-Fabeiro, Francisco Javier; Del Río-Noriega, Francisco; Perez-Perez, Fermin; Haro-González, Jesus; de Diego-Otero, Yolanda; Romero-Sanchiz, Pablo; Moreno-Küstner, Berta; Cichon, Sven; Nöthen, Markus M; Rietschel, Marcella; Mayoral, Fermin

    2017-06-12

    Here, we present the first description of the Andalusian Bipolar Family (ABiF) Study. This longitudinal investigation of families from Andalusia, Spain commenced in 1997 with the aim of elucidating the molecular genetic causes of bipolar affective disorder. The cohort has since contributed to a number of key genetic findings, as reported in international journals. However, insight into the genetic underpinnings of the disorder in these families remains limited. In the initial 1997-2003 study phase, 100 multiplex bipolar disorder and other mood disorder families were recruited. The ongoing second phase of the project commenced in 2013, and involves follow-up of a subgroup of the originally recruited families. The aim of the follow-up investigation is to generate: i) longitudinal clinical data; ii) results from detailed neuropsychological assessments; and iii) a more extensive collection of biomaterials for future molecular biological studies. The ABiF Study will thus generate a valuable resource for future investigations into the aetiology of bipolar affective disorder; in particular the causes of high disease loading within multiply affected families. We discuss the value of this approach in terms of new technologies for the identification of high-penetrance genetic factors. These new technologies include exome and whole genome sequencing, and the use of induced pluripotent stem cells or model organisms to determine functional consequences. Copyright © 2017 SEP y SEPB. Publicado por Elsevier España, S.L.U. All rights reserved.

  15. Polyphenolic content and antioxidant activity of an extractive fraction from Abies alba bark.

    Science.gov (United States)

    Vasincu, Al; Creţu, Elena; Geangalău, Ioana; Amalinei, Roxana Laura Mihăilescu; Miron, Anca

    2013-01-01

    The wood of mature Abies alba Mill. (silver fir) trees is used in construction and furniture industries, the bark being the main waste product. This study aimed to investigate possible therapeutic applications of Abies alba bark waste. A raw extract and four extractive fractions were obtained from silver fir bark. They were also studied regarding their polyphenolic content (Folin-Ciocalteu assay). The ethyl acetate extractive fraction was further studied regarding its ability to scavenge free radicals (DPPH, superoxide anion and hydroxyl radicals) and to chelate ferrous ions. The ethyl acetate fraction contained the highest amount of polyphenols (47.72 +/- 0.38 g gallic acid equivalents/100 g). Its DPPH scavenging and ferrous ions chelating abilities (EC50 = 7.9 +/- 0.1 and 1.56 +/- 0.05 microg/ml) were comparable to those of the positive controls, catechin (EC50 = 7.10 +/- 0.05 microg/ml) and EDTA-Na2 (EC50 = 1.27 +/- 0.01 microg/ml), respectively. It also scavenged superoxide anion and hydroxyl radicals with EC50 values of 53.30 +/- 5.91 and 63.12 +/- 1.78 microg/ml, respectively. The ability of the ethyl acetate fraction to scavenge free radicals and chelate ferrous ions justifies further studies on the possible therapeutic applications of its antioxidant potential.

  16. Improvement of grafting procedures for the ornamental species: II. Abies concolor [(Gord. & Glend. Lindl

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2012-06-01

    Full Text Available The achieved results concerning the grafting silver-fir - Abies concolor [(Gord. & Glend. Lindl] scions on white-fir (Abies alba Mill. rootstocks are reporting in this article. The double-side-veneer grafting method and the plastic tape and the ecological CeraltinŽ wax were applied in four experimental variants. The side-veneer-grafting method and the classic materials, such as raffia and the hot wax were used at the two controls involved in this experiment. The grafting success expressed in percents, were transformed in arcsin square root of percent values, and a two-way analysis of variance was performed. Highly significant (p < 0.001 statistical differences were found between grafting variants, including controls. The Duncan Multiple Range Test showed that the four experimental grafting variants were highly significantly (p < 0.01 be-tter than the two controls. The grafting success of the best experimental variant has surpassed the two controls by 129 and 153%, respectively. Consequently, the double-side-veneer grafting method, the new developed plastic tape and the ecological CeraltinŽ wax have contributed to this grafting success owing to which they are recommended to be used for grafting silver-fir ornamental trees.

  17. Ectomycorrhizal fungal assemblages of Abies alba Mill. outside its native range in Poland.

    Science.gov (United States)

    Rudawska, Maria; Pietras, Marcin; Smutek, Iwona; Strzeliński, Paweł; Leski, Tomasz

    2016-01-01

    Abies alba (Mill.) is an important forest tree species, native to the mountainous regions of Europe but has been also widely introduced in the lowlands outside its native range. Like most forest tree species, A. alba forms obligate mutualisms with ectomycorrhizal (ECM) fungi. This investigation sought to examine ECM fungal communities of A. alba when the species grows 400 km north of its native range in the region of Pomerania in Poland. We surveyed for ECM fungi by sampling live roots from four mature forest stands where the A. alba component ranged from 20 to 100%. Ectomycorrhizal fungal symbionts were identified based on morphotyping and sequencing of the internal transcribed spacer (ITS) of nuclear ribosomal DNA (rDNA). Thirty-five ECM fungal taxa were distinguished on root tips of A. alba from all tested stands with 22 to 27 ECM fungal taxa in the individual stand. The diversity and similarity metrics revealed a lack of statistical differences in the structure of the ECM fungal community between stands varying in overstory tree composition. Cenococcum geophilum was the most common fungal species at all investigated A. alba stands, with an abundance of 50 to 70%. The ECM community was characterized by the lack of Abies-specific fungal symbionts and a rich and diverse suite of host-generalist mycobionts that seem to be sufficient for successful growth and development of A. alba outside of its native range.

  18. Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers

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    Kovačević Dragan

    2013-01-01

    Full Text Available Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%. The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus. [Projekat Ministarstva nauke Republike Srbije, br.173029

  19. The dependence of water potential in shoots of Picea abies on air and soil water status

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    A. Sellin

    Full Text Available Where there is sufficient water storage in the soil the water potential (Ψx in shoots of Norway spruce [Picea abies (L. Karst.] is strongly governed by the vapour pressure deficit of the atmosphere, while the mean minimum values of Ψx usually do not drop below –1.5 MPa under meteorological conditions in Estonia. If the base water potential (Ψb is above –0.62 MPa, the principal factor causing water deficiency in shoots of P. abies may be either limited soil water reserves or atmospheric evaporative demand depending on the current level of the vapour pressure deficit. As the soil dries the stomatal control becomes more efficient in preventing water losses from the foliage, and the leaf water status, in turn, less sensitive to atmospheric demand. Under drought conditions, if Ψb falls below –0.62 MPa, the trees' water stress is mainly caused by low soil water availability. Further declines in the shoot water potential (below –1.5 MPa can be attributed primarily to further decreases in the soil water, i.e. to the static water stress.Key words. Hydrology (evapotranspiration · plant ecology · soil moisture.

  20. Fine roots in stands of Fagus sylvatica and Picea abies along a gradient of soil acidification

    International Nuclear Information System (INIS)

    Braun, Sabine; Cantaluppi, Leonardo; Flueckiger, Walter

    2005-01-01

    Root length of naturally grown young beech trees (Fagus sylvatica L.) was investigated in 26 forest plots of differing base saturation and nitrogen deposition. The relative length of finest roots (<0.25 mm) was found to decrease in soils with low base saturation. A similar reduction of finest roots in plots with high nitrogen deposition was masked by the effect of base saturation. The formation of adventitious roots was enhanced in acidic soils. The analysis of 128 soil profiles for fine roots of all species present in stands of either Fagus sylvatica L., Picea abies [Karst.] L. or both showed a decreased rooting depth in soils with ≤20% base saturation and in hydromorphic soils. For base rich, well drained soils an average rooting depth of 108 cm was found. This decreased by 28 cm on acidic, well drained soils. The results suggest an effect of the current soil acidification in Switzerland and possibly also of nitrogen deposition on the fine root systems of forest trees. - Fine root length of Fagus sylvatica and fine root depth in stands of Fagus sylvatica and/or Picea abies were impaired in soils with low base saturation

  1. Parameters Influencing Tracheostomy Decannulation in Patients Undergoing Rehabilitation after severe Acquired Brain Injury (sABI).

    Science.gov (United States)

    Perin, Cecilia; Meroni, Roberto; Rega, Vincenzo; Braghetto, Giacomo; Cerri, Cesare Giuseppe

    2017-10-01

    Introduction  Tracheostomy weaning in patients who suffered a severe acquired brain injury is often a challenge and decannulation failures are not uncommon. Objective  Our study objective is to describe the decannulation failure rate in patients undergoing rehabilitation following a severe acquired brain injury (sABI); to describe the factors associated with a successful tube weaning. Methods  We conduct a retrospective analysis of charts, consecutively retrieved considering a 3-year window. Variables analyzed were: age, sex, body mass index (BMI), Glasgow Coma Scale (GCS), cause of hospitalization (stroke, trauma, cardiac arrest), date of the pathological event, gap between the index event and the first day of hospitalization, duration of Neurorehabilitation Ward hospitalization, comorbidities, chest morphological alteration, kind of tracheostomy tube used (overall dimension, cap, fenestration), SpO2, presentation and quantification of pulmonary secretion, maximal inspiratory pressure (MIP) and maximal expiratory pressure (MEP), respiratory frequency and pattern, cardiac frequency, presence of spontaneous cough, cough strength, and blood gas analysis. Results  We analyzed 45 tracheostomised sABI patients following stroke, trauma, or cardiac arrest. The weaning success percentage was higher in Head Trauma patients and in patients presenting positive spontaneous cough. Failures seem to be associated with presence of secretions and anoxic brain damage. GCS seemed not related to the decannulation outcome. Conclusions  Parameters that could be used as positive predictors of weaning are: mean expiratory pressure, presence of spontaneous cough, and cough strength. Provoked cough and GCS were not predictive of weaning success.

  2. cDNA amplification by SMART-PCR and suppression subtractive hybridization (SSH)-PCR.

    Science.gov (United States)

    Hillmann, Andrew; Dunne, Eimear; Kenny, Dermot

    2009-01-01

    The comparison of two RNA populations that differ from the effects of a single-independent variable, such as a drug treatment or a specific genetic defect, can identify differences in the abundance of specific transcripts that vary in a population-dependent manner. There are a variety of methods for identifying differentially expressed genes, including microarray, SAGE, qRT-PCR, and DDGE. This protocol describes a potentially less sensitive yet relatively easy and cost-effective alternative that does not require prior knowledge of the transcriptomes under investigation and is particularly applicable when minimal levels of starting material, RNA, are available. RNA input can often be a limiting factor when analyzing RNA from, for example, rigorously purified blood cells. This protocol describes the use of SMART-PCR to amplify cDNA from sub-microgram levels of RNA. The amplified cDNA populations under comparison are then subjected to suppression subtractive hybridization (SSH-PCR), a technique that couples subtractive hybridization with suppression PCR to selectively amplify fragments of differentially expressed genes. The final products are cDNA populations enriched for significantly over-represented transcripts in either of the two input RNA preparations. These cDNA populations may then be cloned to make subtracted cDNA libraries and/or used as probes to screen subtracted cDNA, global cDNA, or genomic DNA libraries.

  3. In vivo antitussive activity of Coccinia grandis against irritant aerosol and sulfur dioxide-induced cough model in rodents

    Directory of Open Access Journals (Sweden)

    Shakti Prasad Pattanayak and Priyashree Sunita

    2009-12-01

    Full Text Available Coccinia grandis (Cucurbitaceae has extensively used to get relief from asthma and cough by the indigenous people of India. The antitussive effect of aerosols of two different concentrations (2.5%, 5% w/v of methanol extract of C. grandis fruits were tested by counting the numbers of coughs produced due to aerosols of citric acid, 10 min after exposing the male guinea pigs to aerosols of test solutions for 7 min. In another set of experiment methanol extract was investigated for its therapeutic efficacy on a cough model induced by sulfur dioxide gas in mice. The results showed significant reduction of cough number obtained in the presence of both concentrations of methanol extract as that of the prototype antitussive agent codeine phosphate. Also, methanol extract exhibited significant antitussive effect at 100, 200 and 400 mg/kg, per orally by inhibiting the cough by 20.57, 33.73 and 56.71% within 90 min of performing the experiment respectively.

  4. Photosynthetic and anatomical responses of Eucalyptus grandis leaves to potassium and sodium supply in a field experiment.

    Science.gov (United States)

    Battie-Laclau, Patricia; Laclau, Jean-Paul; Beri, Constance; Mietton, Lauriane; Muniz, Marta R Almeida; Arenque, Bruna Cersózimo; DE Cassia Piccolo, Marisa; Jordan-Meille, Lionel; Bouillet, Jean-Pierre; Nouvellon, Yann

    2014-01-01

    Although vast areas in tropical regions have weathered soils with low potassium (K) levels, little is known about the effects of K supply on the photosynthetic physiology of trees. This study assessed the effects of K and sodium (Na) supply on the diffusional and biochemical limitations to photosynthesis in Eucalyptus grandis leaves. A field experiment comparing treatments receiving K (+K) or Na (+Na) with a control treatment (C) was set up in a K-deficient soil. The net CO2 assimilation rates were twice as high in +K and 1.6 times higher in +Na than in the C as a result of lower stomatal and mesophyll resistance to CO2 diffusion and higher photosynthetic capacity. The starch content was higher and soluble sugar was lower in +K than in C and +Na, suggesting that K starvation disturbed carbon storage and transport. The specific leaf area, leaf thickness, parenchyma thickness, stomatal size and intercellular air spaces increased in +K and +Na compared to C. Nitrogen and chlorophyll concentrations were also higher in +K and +Na than in C. These results suggest a strong relationship between the K and Na supply to E. grandis trees and the functional and structural limitations to CO2 assimilation rates. © 2013 John Wiley & Sons Ltd.

  5. Amenizante Orgânico e Eucalyptus grandis para Fitoestabilização de Solo Contaminado com Cobre

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    Rudinei De Marco

    2017-04-01

    Full Text Available RESUMO Este trabalho avaliou a utilização de turfa como amenizante e Eucalyptus grandis como técnica de fitoestabilização para remediar solo contaminado com cobre. O trabalho foi conduzido sob delineamento inteiramente casualizado em arranjo fatorial (2 × 6, sendo sem e com adição de turfa (200 mL L-1 de solo e seis doses de cobre adicionadas ao solo (0 mg kg-1, 60 mg kg-1, 120 mg kg-1, 180 mg kg-1, 240 mg kg-1, 300 mg kg-1 de solo, com seis repetições. Avaliaram-se a altura das mudas, o diâmetro do colo, a massa seca radicular e aérea, a área superficial específica, os teores e a quantidade acumulada de cobre no sistema radicular e na parte aérea e o índice de translocação. A adição de turfa possibilitou efeito amenizante da contaminação por cobre e favoreceu o crescimento das mudas. As mudas de Eucalyptus grandis apresentam elevado acúmulo de cobre nas raízes, o que permite indicá-la como promissora para fins de fitoestabilização de solos contaminados com cobre.

  6. A study of the bonding of wood from Eucalyptus grandis W. Hill ex Maiden using resorcinol-formaldehyde adhesive

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    Vanessa Cristina do Sacramento Albino

    2010-12-01

    Full Text Available This work aims to evaluate the strength of joints bonded with thermosetting resorcinol-formaldehyde adhesive, as obtained from three radial and three longitudinal positions relative to the log in wood of Eucalyptus grandis W. Hill ex Maiden (Myrtaceae at age 18 years, and also to correlate it with basic density. The adhesive spread rate was 250 g/m2 using double lines and adding hardener at a rate of one part of hardener to five parts of adhesive. Moisture content in the wood sheets at the time of bonding was 12%. Bonded joints were prepared according to standard ASTM D 2339-98. Basic density values showed an increasing tendency in the pith-to-bark direction while no significant variation was noted in the base-to-top direction. Higher mean values of shear strength and smaller percentages of wood failure were obtained from joints originated in positions close to the bark and to the log base. Overall, the bonding behavior of resorcinol-formaldehyde adhesive in Eucalyptus grandis was found to vary considerably in different positions relative to the log.

  7. [Craniometrical study of the species complex of Meriones shawii-grandis (Mammalia: Rodentia) in Morocco, in Algeria and in Tunisia].

    Science.gov (United States)

    Djelaila, Yassine; Denys, Christiane; Stoetzel, Emmanuelle; Cornette, Raphaël; Lalis, Aude; Adamou-Djerbaoui, Malika; Boukhemza, Mohamed

    2018-01-01

    In North Africa, the rodents of the species complex Meriones shawii-grandis have a considerable ecological, economic and epidemiological importance. Until now, the systematics of these species was subject to discussion due to the presence of populations displaying high morphological variability. By means of an approach of traditional morphometrics based on cranial distances and by using the method of the log shape-ratio, we attempt to characterize morphologically these two taxa. The results show significant differences in size and shape between the specimens of Morocco, on the one hand, and those of Algeria and Tunisia, on the other hand. The samples of Morocco that have been molecularly typed and attributed to M. grandis have larger tooth rows and narrower skulls, as well as relatively small tympanic bullae. On the other hand, those of Algeria and Tunisia assigned to M. shawii are characterized by small tooth rows and wide skulls with well-developed tympanic bullae. The morphological distance is relatively strong between both clades (79.5%), which corresponds to the molecular distance. However, the discriminant analysis performed after molecularly-typed specimens allows the correct classification of only 91.8% of the individuals. Copyright © 2017 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.

  8. α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark

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    Ping Jiang

    2017-01-01

    Full Text Available This study evaluated the inhibitory effects of different extracts and fractions from Eucalyptus. grandis × urophylla bark (EB against α-glucosidase and α-amylase enzyme activities. The ethyl acetate extract (EB-E showed the highest activity among others. Seven fractions were derived from EB-E; among them EB-E-7 showed the highest significant inhibition of both enzymes, with IC50 of 1.40±0.18 and 1.72±0.12 μg/mL, respectively. EB-E and its active fraction EB-E-7 showed highest contents of total phenolics: 178.79±4.68 and 920.4±5.46 mg GAEag−1, respectively. HPLC-MS analysis of EB-E-7 revealed the presence of ellagic acid, quercetin-glucuronide, quercetin-3-α-rhamnopyranoside, and ellagic acid rhamnoside as major compounds, together with smaller concentrations of myricetin-rhamnoside, isorhamnetin-hexoside, myricetin-3-α-arabinofuranoside, and isorhamnetin. Therefore, the phenolic compounds from Eucalyptus grandis × E. urograndis bark potently inhibited α-amylase and α-glucosidase activity, having potential in prevention of hyperglycemia.

  9. Genome-wide characterization and expression profiling of the AUXIN RESPONSE FACTOR (ARF gene family in Eucalyptus grandis.

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    Hong Yu

    Full Text Available Auxin is a central hormone involved in a wide range of developmental processes including the specification of vascular stem cells. Auxin Response Factors (ARF are important actors of the auxin signalling pathway, regulating the transcription of auxin-responsive genes through direct binding to their promoters. The recent availability of the Eucalyptus grandis genome sequence allowed us to examine the characteristics and evolutionary history of this gene family in a woody plant of high economic importance. With 17 members, the E. grandis ARF gene family is slightly contracted, as compared to those of most angiosperms studied hitherto, lacking traces of duplication events. In silico analysis of alternative transcripts and gene truncation suggested that these two mechanisms were preeminent in shaping the functional diversity of the ARF family in Eucalyptus. Comparative phylogenetic analyses with genomes of other taxonomic lineages revealed the presence of a new ARF clade found preferentially in woody and/or perennial plants. High-throughput expression profiling among different organs and tissues and in response to environmental cues highlighted genes expressed in vascular cambium and/or developing xylem, responding dynamically to various environmental stimuli. Finally, this study allowed identification of three ARF candidates potentially involved in the auxin-regulated transcriptional program underlying wood formation.

  10. NITROGEN SOURCES AND DOSES ON GROWTH AND QUALITY OF SEEDLINGS OF Cassia grandis AND Peltophorum dubium 1

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    Cássia Barreto Soares

    2017-02-01

    Full Text Available ABSTRACT The demand for seedlings of Brazilian native tree species has increased due to the growing number of recovery projects for degraded areas. In order to obtain success in the revegetation, high quality seedlings should be used. Among the factors that affect the quality of seedlings there is nitrogen fertilization, since nitrogen (N is one of the nutrients required in the largest amount by plants. Thus, this study aimed to evaluate the response of Cassia grandis and Peltophorum dubium seedlings to N sources and doses. The treatments were represented by a factorial of three sources (ammonium nitrate, calcium nitrate and ammonium sulfate by five N doses (0, 50, 100, 150 and 200 mg dm-3, arranged in randomized complete block design, with four replicates. After 145 days of sowing, were obtained: height (H, collar diameter (CD, shoot dry matter (SDM, root dry matter (RDM, total dry matter (TDM, and calculated the dry matter mass ratio of shoot and root (SDM/RDM and the Dickson Quality Index (DQI. Nitrogen fertilization resulted in growth gains and improved the seedlings quality of both species. However, the sources and the source × doses interaction were not significant for all variables. For the studied substrate (Red-Yellow Latosol the recommended dose is 185 mg dm-3 of N for the production of Peltophorum dubium seedlings. For the production of Cassia grandis seedlings was not able to find the optimum dose of N.

  11. Tectona grandis leaf extract, free and associated with nanoemulsions, as a possible photosensitizer of mouse melanoma B16 cell.

    Science.gov (United States)

    de Menezes Furtado, Cydia; de Faria, Fernando Sergio Escocio Drumond Viana; Azevedo, Ricardo Bentes; Py-Daniel, Karen; Dos Santos Camara, Ana Lygia; da Silva, Jaqueline Rodriguez; de Holanda Oliveira, Everton; Rodriguez, Anselmo Fortunato Ruiz; Degterev, Igor Anatolievich

    2017-02-01

    Over the past six years we have been studying extracts from tropical, specially Amazon, plants, to search for new sensitizers for photodynamic therapy of cancer and infectious diseases. Tectona grandis is a genus of tropical hardwood trees in the mint family, Lamiaceae. That is native to south and southeast Asia, but since the end of the 20th century is also gaining ground in the Amazon. The present work aims to evaluate the photodynamic potential of hydro-alcoholic extract from Tectona grandis LF leaves (TGE) and the same extract prepared as the oil-water nanoemulsion (TGE-NE) against melanoma B16 F10 cells. The method for preparation of a stable nanoemulsion with ~20nm particles associated to the TGE (TGE-NE) was successfully developed. We have shown that both free and nanostructured presentations possess the ability to sensitize B16 F10 cells to red light of the LED in vitro. Photodynamic effect was observed for both TGE and TGE-NE because toxicity increased under illumination with red light. While TGE was highly toxic towards melanoma cells under illumination with red light of the LED, it also possessed significant dark toxicity towards both B16 F10 and murine fibroblast NIH3T3 cells. The TGE-NE showed reasonable photocytotoxicity and was much less toxic towards normal cells in the dark compared to free TGE. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. MICOBIOTA ASSOCIADA À MADEIRA SERRADA DE Eucalyptus grandis HILL EX MAIDEN DURANTE A SECAGEM AO AR LIVRE

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Trugilho

    2006-02-01

    Full Text Available O objetivo principal deste trabalho foi o de acompanhar a secagem ao ar livre da madeira serrada de Eucalyptus grandis conduzida na região de Lavras, MG e identificar a micobiota associada à madeira em diversas fases da secagem. As tábuas foram cortadas de oito toras de 3,0 m de comprimento, oriundas de três árvores, com 27 anos de idade, plantadas em área experimental da UFLA. Dos resultados, pôde-se concluir que a secagem da madeira de Eucalyptus grandis ao ar livre, iniciada em janeiro, consumiu 158 dias até atingir umidade próxima a 12,5%. A curva de secagem foi representada por uma equação logarítmica com coeficiente de determinação (R2 igual a 98,3%. Os fungos causadores de bolores superficiais e de manchas tiveram maior ocorrência no início da secagem. As mais altas freqüências de fungos, verificadas no início da secagem, foram observadas para Penicillium spp. e Pestalotiopsis sp. O fungo Lentinus lepideus ocorreu com maior freqüência no final da secagem.

  13. The Eucalyptus grandis R2R3-MYB transcription factor family: evidence for woody growth-related evolution and function.

    Science.gov (United States)

    Soler, Marçal; Camargo, Eduardo Leal Oliveira; Carocha, Victor; Cassan-Wang, Hua; San Clemente, Hélène; Savelli, Bruno; Hefer, Charles A; Paiva, Jorge A Pinto; Myburg, Alexander A; Grima-Pettenati, Jacqueline

    2015-06-01

    The R2R3-MYB family, one of the largest transcription factor families in higher plants, controls a wide variety of plant-specific processes including, notably, phenylpropanoid metabolism and secondary cell wall formation. We performed a genome-wide analysis of this superfamily in Eucalyptus, one of the most planted hardwood trees world-wide. A total of 141 predicted R2R3-MYB sequences identified in the Eucalyptus grandis genome sequence were subjected to comparative phylogenetic analyses with Arabidopsis thaliana, Oryza sativa, Populus trichocarpa and Vitis vinifera. We analysed features such as gene structure, conserved motifs and genome location. Transcript abundance patterns were assessed by RNAseq and validated by high-throughput quantitative PCR. We found some R2R3-MYB subgroups with expanded membership in E. grandis, V. vinifera and P. trichocarpa, and others preferentially found in woody species, suggesting diversification of specific functions in woody plants. By contrast, subgroups containing key genes regulating lignin biosynthesis and secondary cell wall formation are more conserved across all of the species analysed. In Eucalyptus, R2R3-MYB tandem gene duplications seem to disproportionately affect woody-preferential and woody-expanded subgroups. Interestingly, some of the genes belonging to woody-preferential subgroups show higher expression in the cambial region, suggesting a putative role in the regulation of secondary growth. © 2014 The Authors New Phytologist © 2014 New Phytologist Trust.

  14. Validation of use of a traditional remedy from Bridelia grandis (Pierre ex Hutch) stem bark against oral Streptococci.

    Science.gov (United States)

    Ngueyem, T A; Brusotti, G; Marrubini, G; Grisoli, P; Dacarro, C; Vidari, G; Finzi, P Vita; Caccialanza, G

    2008-10-30

    Bridelia grandis (Pierre ex Hutch) (Euphorbiaceae) is a medicinal plant traditionally used in Cameroon by pygmies Baka as a remedy for oral cavity affection. Bioassay-guided stem bark extracts were investigated for their in vitro antimicrobial properties as well as their phytochemical constituents. The first extraction was carried out according to the traditional use. Further extractions were carried out with solvents of different polarity such as methanol (MeOH), ethyl acetate (EtOAc) and mixtures of MeOH-H2O. The antimicrobial activity of the extracts against oral Streptococci was evaluated on the basis of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) by the macrodilution method; the bacterial surface hydrophobicity was also evaluated. Water, methanol and mixtures methanol-water extracts, exhibited antibacterial activity with MIC between 0.5 and 2mg/ml justifying the traditional use of Bridelia grandis stem bark for oral cavity affection. Preliminary phytochemical analysis was performed on the most active extract (methanol) using appropriate tests and well established analytical screening methods, such as TLC and RP-HPLC/DAD. The data obtained indicate that tannins constitute the chemical family responsible for the biological activity.

  15. [Construction of a cDNA library from liver tissue of rhesus monkey, Macaca mulatta].

    Science.gov (United States)

    Qin, Sheng-fang; Tan, Wei-dong; Chen, You-nan; Ding, Yang; Li, Sheng-fu; Li, Hong-xia; Wang, Li; Yang, Rong; Lu, Yan-rong

    2007-06-01

    To screen the target rhesus genes and give some basic genetic evidences to its value as one of the most important animal model in biomedical study, we constructed a cDNA expression library from liver tissue of a healthy rhesus monkey. With Trizol reagent, the total RNA was extracted from healthy rhesus liver tissue. By mutant Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT), the first-strand cDNA was synthesized from purified mRNA, and subsequently the second-strand cDNA was generated via E. coli DNA polymerase I . Then, the EcoR I adapter was added to the synthesized double-strand cDNA, which was subsequently digested by Xho I restriction enzyme and fractionated with CHROMA APIN-400 column. The fractionated cDNA fragments to be longer than 0. 5 kb were ligated into lambda ZAP express vector to form the phagemid cDNA recombinants, which were further packaged into the lambda ZAP cDNA library according to the standard protocol with phage lambda Gold packaging extract. In order to get more stable clones with larger quantity, the primary library was amplified through infecting the host strain XL1-Blue MRF'. Then, the library titre, recombinant rate and length of inserted cDNA were measured, respectively. The capacity of the primary stand or unamplified library was 1. 2X 10(6) pfu. The titers of the unamplified library or the amplified library was 1.1 X 10(6) mixture, pfu/mL or 7. 7 X 10(9) pfu/mL respectively, the percentages of recombinants were 99. 3% and 98. 2%, and the average lengths of the inserts were 2.0 kb and 2. 3 kb, respectively. An excellent cDNA expression library has been constructed successfully, which would lay solid foundation for transplantation study and pre-clinic evaluation of related drugs.

  16. [cDNA libraries construction and screening in gene expression profiling of disease resistance in wheat].

    Science.gov (United States)

    Luo, Meng; Kong, Xiu-Ying; Liu, Yue; Zhou, Rong-Hua; Jia, Ji-Zeng

    2002-09-01

    A wheat line, Bai Nong 3217/Mardler BC5F4 with resistance to powdery mildew, was used to construct a conventional cDNA library and a suppression subtractive hybridization (SSH) cDNA library from wheat leaves inoculated by Erysiphe graminis DC. Three hundred and eighty-seven non-redundant ESTs from the conventional cDNA library and 760 ESTs from the SSH cDNA library were obtained, and the ESTs similarity analysis using BLASTn and BLASTx were conducted by comparing these ESTs with sequences in GenBank. The results showed that the redundancy of some kinds of genes such as photosynthesis related genes and ribosome related genes was higher in the conventional cDNA library but the varieties and quantities of disease resistance genes were less than those in the SSH cDNA library. The SSH cDNA library was found to have obvious advantages in gene expression profiling of disease resistance such as simple library construction procedure, rich specific DRR (disease-resistance-related) genes and decreased sequencing amount. To acquire genes that were involved in the powdery mildew resistance of wheat, hybridization with high-density dots membranes was used to screen the two libraries. The result showed that the method was relatively simple in operation, and the membranes could be used for many times. But some problems also existed with this screening method. For instance, a large amount of mRNA and radioactive isotope were needed and the hybridization procedure must be repeated several times to obtain stable hybridization results. About 54.1% function-known ESTs in the SSH cDNA library were identified to be DRR genes by screening. There were 247 clones of the SSH cDNA library that had positive signal in the repeated hybridizations with the pathogen uninfected probe. The identified DRR genes distributed in the whole procedure of powdery mildew resistance, but mainly focused on the SAR (systemic of acquired resistance).

  17. The isolation and amplification of full length cDNA of oleosins from ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-03-29

    Mar 29, 2010 ... Subcloning of DNA by inserting into pCR® 4-TOPO vector was performed using TOPO TA Cloning® Kit for Sequencing. (Invitrogen, USA). The sequencing of plasmid clones was performed using ABI PRISMTM Dye Terminator Cycle Sequencing Ready. Reaction Kit (Applied Biosystems Inc., USA) and an ...

  18. Isolation of cDNA clones coding for human tissue factor: primary structure of the protein and cDNA

    International Nuclear Information System (INIS)

    Spicer, E.K.; Horton, R.; Bloem, L.

    1987-01-01

    Tissue factor is a membrane-bound procoagulant protein that activates the extrinsic pathway of blood coagulation in the presence of factor VII and calcium. λ Phage containing the tissue factor gene were isolated from a human placental cDNA library. The amino acid sequence deduced from the nucleotide sequence of the cDNAs indicates that tissue factor is synthesized as a higher molecular weight precursor with a leader sequence of 32 amino acids, while the mature protein is a single polypeptide chain composed of 263 residues. The derived primary structure of tissue factor has been confirmed by comparison to protein and peptide sequence data. The sequence of the mature protein suggests that there are three distinct domains: extracellular, residues 1-219; hydrophobic, residues 220-242; and cytoplasmic, residues 243-263. Three potential N-linked carbohydrate attachment sites occur in the extracellular domain. The amino acid sequence of tissue factor shows no significant homology with the vitamin K-dependent serine proteases, coagulation cofactors, or any other protein in the National Biomedical Research Foundation sequence data bank (Washington, DC)

  19. Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli.

    OpenAIRE

    Goddard, J M; Caput, D; Williams, S R; Martin, D W

    1983-01-01

    We have obtained cDNA clones that contain the entire coding region of the human purine-nucleoside phosphorylase (PNP; EC 2.4.2.1) mRNA. The cDNA sequences were generated by reverse transcription of PNP-enriched mRNA obtained by immunoadsorption of HeLa cell polyribosomes with monospecific antibody to human PNP. cDNA molecules that were close in length to PNP mRNA were separated by agarose gel electrophoresis and inserted into the Pst I site of the plasmid pBR322. Plasmid DNA from the pooled c...

  20. Generation of cDNA expression libraries enriched for in-frame sequences

    OpenAIRE

    Davis, Claytus A.; Benzer, Seymour

    1997-01-01

    Bacterial cDNA expression libraries are made to reproduce protein sequences present in the mRNA source tissue. However, there is no control over which frame of the cDNA is translated, because translation of the cDNA must be initiated on vector sequence. In a library of nondirectionally cloned cDNAs, only some 8% of the protein sequences produced are expected to be correct. Directional cloning can increase this by a factor of two, but it does not solve the frame problem. We have therefore deve...

  1. Generation of full-length cDNA libraries: focus on plants.

    Science.gov (United States)

    Seki, Motoaki; Kamiya, Asako; Carninci, Piero; Hayashizaki, Yoshihide; Shinozaki, Kazuo

    2009-01-01

    Full-length cDNAs are essential for the correct annotation of transcriptional units and gene products from genomic sequence data and for functional analysis of the genes. Full-length cDNA libraries are very important resources for isolation of the full-length cDNAs. The biotinylated cap trapper method using the trehalose-thermostabilized reverse transcriptase has been developed and has become an efficient method for construction of high-content full-length cDNA libraries. We have constructed full-length cDNA libraries from various plants and animals using this method. The protocol of the method is described in this chapter.

  2. Cloning and sequencing of dolphinfish (Coryphaena hippurus, Coryphaenidae) growth hormone-encoding cDNA.

    Science.gov (United States)

    Peduel, A D; Elizur, A; Knibb, W

    1994-01-01

    The cDNA encoding the preprotein growth hormone from the dolphinfish (Coryphaena hippurus) has been cloned and sequenced. The cDNA was derived by reverse transcription of RNA from the pituitary of a young fish using the method known as Rapid Amplification of cDNA Ends (RACE). An oligonucleotide primer corresponding to the 5' region of Pagrus major and the universal RACE primer enabled amplification using the Polymerase Chain Reaction (PCR). The dolphinfish and yellow-tail, Seriola quineqeradiata, are both members of the sub-order Percoidei (Perciforme) and their GH sequences show a high level of homology.

  3. Xylan-hydrolyzing enzyme system from Bacillus pumilus CBMAI 0008 and its effects on Eucalyptus grandis kraft pulp for pulp bleaching improvement.

    Science.gov (United States)

    Duarte, Marta C Teixeira; da Silva, Elizete Cristina; de Bulhões Gomes, Isabel Menezes; Ponezi, Alexandre Nunes; Portugal, Edilberto Princi; Vicente, João Roberto; Davanzo, Ednilson

    2003-05-01

    The extracellular productions of beta-xylanase, beta-xylosidase, beta-glucosidase, beta-mannanase, arabinosidase, alpha-glucuronidase, alpha-galactosidase and Fpase from Bacillus pumilus CBMAI 0008 were investigated with three different xylan sources as substrate. The enzymatic profiles on birchwood, Eucalyptus grandis and oat were studied at alkaline and acidic pH conditions. B. pumilus CBMAI 0008 grown on the three carbon sources produced mainly beta-xylanase. At pH 10, the levels of xylanase were 328, 160 and 136 U/ml, for birch, oat and E. grandis, respectively. beta-Mannanase production was induced on E. grandis (5 U/ml) and arabinofuranosidase on oat (5 U/ml). Although small quantities of alpha-glucuronidase had been produced at pH 10, activity at pH 4.8 was 1.5 U/ml, higher than observed for Aspergillus sp. in literature reports. Preliminary assays carried out on E. grandis kraft pulp from an industrial paper mill (RIPASA S.A. Celulose e Papel, Limeira, SP, Brazil) showed a reduction of 0.3% of chlorine use in the pulp treated with the enzymes, resulting in increased brightness, compared to conventional bleaching. The enzymes were more efficient if applied before the initial bleaching sequence, in a non-pre-oxygenated pulp.

  4. A technique to identify annual growth rings in Eucalyptus grandis using annual measurements of diameter at breast height and gamma ray densitometry

    CSIR Research Space (South Africa)

    Naidoo, Sasha

    2010-06-01

    Full Text Available of radial variation in wood density within the context of the growth history of a compartment (by means of annual PSP data), it was possible to corroborate the estimation of annual growth rings on density profiles of E. grandis, especially closer to the bark-end....

  5. Microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandis

    CSIR Research Space (South Africa)

    Barros, E

    2009-05-01

    Full Text Available of Eucalyptus grandis planting stock that exhibit preferred wood qualities is thus a priority of the South African forestry industry. The researchers used microarray-based DNA-amplified fragment length polymorphism (AFLP) analysis in combination with expression...

  6. 5'-end sequences of budding yeast full-length cDNA clones and quality scores - Budding yeast cDNA sequencing project | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us Budding yeast cDNA sequencing project 5'-end sequences of budding yeast full-length cDNA clones and quality... scores Data detail Data name 5'-end sequences of budding yeast full-length cDNA clones and quality...or-capping method, the sequence quality score generated by the Phred software, and links to SGD, dbEST and U...es. FASTA format. Quality Phred's quality score About This Database Database Desc...g yeast full-length cDNA clones and quality scores - Budding yeast cDNA sequencing project | LSDB Archive ...

  7. Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae.

    Science.gov (United States)

    Artico, Sinara; Ribeiro-Alves, Marcelo; Oliveira-Neto, Osmundo Brilhante; de Macedo, Leonardo Lima Pepino; Silveira, Sylvia; Grossi-de-Sa, Maria Fátima; Martinelli, Adriana Pinheiro; Alves-Ferreira, Marcio

    2014-10-04

    Cotton is a major fibre crop grown worldwide that suffers extensive damage from chewing insects, including the cotton boll weevil larvae (Anthonomus grandis). Transcriptome analysis was performed to understand the molecular interactions between Gossypium hirsutum L. and cotton boll weevil larvae. The Illumina HiSeq 2000 platform was used to sequence the transcriptome of cotton flower buds infested with boll weevil larvae. The analysis generated a total of 327,489,418 sequence reads that were aligned to the G. hirsutum reference transcriptome. The total number of expressed genes was over 21,697 per sample with an average length of 1,063 bp. The DEGseq analysis identified 443 differentially expressed genes (DEG) in cotton flower buds infected with boll weevil larvae. Among them, 402 (90.7%) were up-regulated, 41 (9.3%) were down-regulated and 432 (97.5%) were identified as orthologues of A. thaliana genes using Blastx. Mapman analysis of DEG indicated that many genes were involved in the biotic stress response spanning a range of functions, from a gene encoding a receptor-like kinase to genes involved in triggering defensive responses such as MAPK, transcription factors (WRKY and ERF) and signalling by ethylene (ET) and jasmonic acid (JA) hormones. Furthermore, the spatial expression pattern of 32 of the genes responsive to boll weevil larvae feeding was determined by "in situ" qPCR analysis from RNA isolated from two flower structures, the stamen and the carpel, by laser microdissection (LMD). A large number of cotton transcripts were significantly altered upon infestation by larvae. Among the changes in gene expression, we highlighted the transcription of receptors/sensors that recognise chitin or insect oral secretions; the altered regulation of transcripts encoding enzymes related to kinase cascades, transcription factors, Ca2+ influxes, and reactive oxygen species; and the modulation of transcripts encoding enzymes from phytohormone signalling pathways. These

  8. Systematics and evolution of the Meriones shawii/grandis complex (Rodentia, Gerbillinae) during the Late Quaternary in northwestern Africa: Exploring the role of environmental and anthropogenic changes

    Science.gov (United States)

    Stoetzel, Emmanuelle; Cornette, Raphaël; Lalis, Aude; Nicolas, Violaine; Cucchi, Thomas; Denys, Christiane

    2017-05-01

    Rodents of the Meriones shawii/grandis complex have been attested to in North Africa since the Middle Pleistocene and are abundant in archaeological sites. Today, they are widely spread and represent a major pest to local human populations. This complex, therefore, represents an accurate model for investigating the roles of climate change and human impact in shaping Quaternary faunal diversity and distribution. Many gray areas still exist regarding the systematics, ecology and geographical distribution of this complex, for both present and past populations. The purpose of this study is to compare modern genotyped and fossil Meriones specimens in order to 1) clarify the current systematics and distribution of the Meriones populations of the shawii/grandis complex, 2) document the taxonomic diversity in fossil Meriones from northwestern Africa, and 3) track their phenotypic and biogeographic evolution through time. To answer these questions we used geometric morphometrics on skulls (landmarks) and first upper molars (landmarks and sliding landmarks). We evidenced the existence of two morpho-groups within the M. shawii/grandis complex, with a clear geographic pattern (M. grandis in Morocco vs. M. shawii in Algeria and Tunisia). Currently only one morpho-group, attributed to M. grandis, seems to exist in Morocco, with a small overlap with M. shawii in the most eastern part of the country. However, according to fossil data, M. shawii was also present in Atlantic Morocco during the Late Pleistocene. We have also highlighted the impact of Holocene climate change and habitat anthropization on this arid adapted group. During the Middle Holocene, a major climatic event (last interglacial optimum) seems to have induced a demographic collapse in Moroccan populations and the disappearance of the shawii clade from Morocco (except in the most eastern areas). Both species then re-expanded, benefitting from the increasing aridity and the new ecological niche driven by agriculture

  9. Trophic discrimination factor of nitrogen isotopes within amino acids in the dobsonflyProtohermes grandis(Megaloptera: Corydalidae) larvae in a controlled feeding experiment.

    Science.gov (United States)

    Ishikawa, Naoto F; Hayashi, Fumio; Sasaki, Yoko; Chikaraishi, Yoshito; Ohkouchi, Naohiko

    2017-03-01

    The trophic discrimination factor (TDF) of nitrogen isotopes ( 15 N/ 14 N) within amino acids, between a stream-dwelling dobsonfly larva ( Protohermes grandis : Megaloptera; Corydalidae) and its diet (chironomid larvae), was determined in controlled feeding experiments. Last-instar larvae of P. grandis were collected from the Yozawa-gawa River, central Japan, and reared in the laboratory. After fed to satiation for 1 month, one group of larvae was each fed one living chironomid larva per day for 4 weeks, while a second group was starved for 8 weeks. The larvae were harvested at intervals and the nitrogen isotopic composition of glutamic acid ( δ 15 N Glu ) and phenylalanine ( δ 15 N Phe ) were determined to calculate TDF. The mean TDF of satiated and starved larvae were 7.1‰ ± 0.5‰ ( n  = 3) and 7.3‰ ± 0.5‰ ( n  =   5), respectively. Thus, the TDF for P. grandis larvae in this study was similar to that reported for other arthropods (approximately 7‰) and was independent of satiation or starvation. A previous study of wild P. grandis larvae, based on the δ 15 N Glu and δ 15 N Phe values, estimated its trophic position (TP) as approximately 2.0 ± 0.1 ( n  =   5), a low value close to that of algivores, although they are generally characterized as carnivores (usually accepted as TP ≥ 3). The TDF for P. grandis larvae suggests that their low TPs in nature were caused by incorporation of vascular plant-derived amino acids (with a different δ 15 N profile from that of algae) and not by an unusually low TDF or by the effects of the satiation/starvation on amino acid metabolism.

  10. Mouse tetranectin: cDNA sequence, tissue-specific expression, and chromosomal mapping

    DEFF Research Database (Denmark)

    Ibaraki, K; Kozak, C A; Wewer, U M

    1995-01-01

    regulation, mouse tetranectin cDNA was cloned from a 16-day-old mouse embryo library. Sequence analysis revealed a 992-bp cDNA with an open reading frame of 606 bp, which is identical in length to the human tetranectin cDNA. The deduced amino acid sequence showed high homology to the human cDNA with 76...... in human. Although additional minor bands of 1.5 and 3.3 kb were found in Northern blots, RT-PCR (reverse transcription polymerase chain reaction) analysis failed to provide evidence that these minor bands are products of the tetranectin gene. Finally, the genetic map location for this gene, Tna...

  11. Cloning and sequencing of complete τ-crystallin cDNA from ...

    Indian Academy of Sciences (India)

    Unknown

    length τ-crystallin cDNA from crocodilian lens and α-enolase from other tissues. ... human (Acc. No. NM_001428). The sequences were used to construct a phylogenetic tree depicting gene lineage, using the clustering program DNAML.

  12. Construction and characterization of a cDNA expression library from the endangered Hu sheep.

    Science.gov (United States)

    Hu, P-F; Li, X-C; Liu, H-K; Guan, W-J; Ma, Y-H

    2014-10-31

    Hu sheep is one of the most important species in China; it is also listed as one of the 78 nationally protected domestic animals by the Chinese government in 2000. The construction of cDNA expression library of Hu sheep is of great significance for protecting individual genomes, generating transgenic sheep, and conducting clinical research using cDNA from Hu sheep. In this study, the total RNA from the ear tissue of Hu sheep was extracted, and a cDNA expression library was constructed using the SMART(TM) technique. The titer of amplified cDNA library was 1.09 x 10(10) PFU/mL, the rate of recombination was above 91.6%, and the average size of fragments was 1.1 kb. This study has an important significance for the preservation of Hu sheep resources at the genome level.

  13. Mouse tetranectin: cDNA sequence, tissue-specific expression, and chromosomal mapping

    DEFF Research Database (Denmark)

    Ibaraki, K; Kozak, C A; Wewer, U M

    1995-01-01

    regulation, mouse tetranectin cDNA was cloned from a 16-day-old mouse embryo library. Sequence analysis revealed a 992-bp cDNA with an open reading frame of 606 bp, which is identical in length to the human tetranectin cDNA. The deduced amino acid sequence showed high homology to the human cDNA with 76......(s) of tetranectin. The sequence analysis revealed a difference in both sequence and size of the noncoding regions between mouse and human cDNAs. Northern analysis of the various tissues from mouse, rat, and cow showed the major transcript(s) to be approximately 1 kb, which is similar in size to that observed...

  14. cDNA - ASTRA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ing/transcriptional initiation regionss About This Database Database Description Download License Update History of This Database Site Policy | Contact Us cDNA - ASTRA | LSDB Archive ...

  15. Construction of primary and subtracted cDNA libraries from early embryos.

    Science.gov (United States)

    Rothstein, J L; Johnson, D; Jessee, J; Skowronski, J; DeLoia, J A; Solter, D; Knowles, B B

    1993-01-01

    By modifying current cDNA cloning and electroporation methods, large and representative murine cDNA libraries were synthesized from 10 to 100 ng mRNA isolated from unfertilized egg and preimplantation mouse embryos. High cloning efficiency is essential for complete representation of genes expressed in egg and preimplantation embryos and for the isolation of stage-specific genes using subtractive hybridization. Because the mouse embryo contains no more than 50 pg of poly(A)+ mRNA at any stage of preimplantation development, approximately 5000-10,000 embryos are required to obtain enough mRNA to synthesize libraries using current methods. To obtain a representative library that also includes rare transcripts, the size of the library should be at least 10(6) clones. The average percent conversion of mRNA to single-stranded cDNA was 20-40%, so that a cloning efficiency of nearly 2 x 10(8) cfu/microgram cDNA is required for such a cDNA library. No previous methods have provided directional cloning of cDNA into plasmids with these high efficiencies. The advent of electroporation methods for the introduction of nucleic acids into bacteria has made possible the use of standard plasmid vectors for high-efficiency cDNA cloning. Plasmid vectors are currently available that can accommodate the directional cloning of cDNA such that T7 and T3 RNA polymerase promoter sequences can be used to generate sense and anti-sense transcripts for subtractive hybridization and riboprobe synthesis. The cDNA libraries we derived using this methodology are a reusable and abundant source of genetic information about the control of preimplantation development. Specialized subtractive cDNA libraries enriched for genes expressed exclusively at a predetermined time in development give access to genes expressed in a stage-specific manner. The ability to construct new cDNA libraries from limited amounts of starting material ensures the provision of new and important resources for the identification

  16. Radioactive cDNA microarray (II): Gene expression profiling of antidepressant treatment by human cDNA microarray

    International Nuclear Information System (INIS)

    Lee, Ji Hye; Kang, Rhee Hun; Ham, Byung Joo; Lee, Min Su; Shin, Kyung Ho; Choe, Jae Gol; Kim, Meyoung Kon

    2003-01-01

    Major depressive disorder is a prevalent psychiatric disorder in primary care, associated with impaired patient functioning and well-being. Fluoxetine is a selective serotonin-reuptake inhibitors (SSRIs) and is a commonly prescribed antidepressant compound. Its action is primarily attributed to selective inhibition of the reuptake of serotonin (5-hydroxytryptamine) in the central nervous system. Objectives ; the aims of this study were two-fold: (1) to determine the usefulness for investigation of the transcription profiles in depression patients, and (2) to assess the differences in gene expression profiles between positive response group and negative response groups by fluoxetine treatment. This study included 53 patients with major depression (26 in positive response group with antidepressant treatment, 27 in negative response group with antidepressant treatment), and 53 healthy controls. To examine the difference of gene expression profile in depression patients, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 genes in total. Using 33p-labeled probes, this method provided highly sensitive gene expression profiles including brain receptors, drug metabolism, and cellular signaling. Gene transcription profiles were classified into several categories in accordance with the antidepressant gene-regulation. The gene profiles were significantly up-(22 genes) and down-(16 genes) regulated in the positive response group when compared to the control group. Also, in the negative response group, 35 genes were up-regulated and 8 genes were down-regulated when compared to the control group. Consequently, we demonstrated that radioactive human cDNA microarray is highly likely to be an efficient technology for evaluating the gene regulation of antidepressants, such as selective serotonin-reuptake inhibitors (SSRIs), by using high-throughput biotechnology

  17. Radioactive cDNA microarray (II): Gene expression profiling of antidepressant treatment by human cDNA microarray

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ji Hye; Kang, Rhee Hun; Ham, Byung Joo; Lee, Min Su; Shin, Kyung Ho; Choe, Jae Gol; Kim, Meyoung Kon [College of Medicine, Univ. of Korea, Seoul (Korea, Republic of)

    2003-07-01

    Major depressive disorder is a prevalent psychiatric disorder in primary care, associated with impaired patient functioning and well-being. Fluoxetine is a selective serotonin-reuptake inhibitors (SSRIs) and is a commonly prescribed antidepressant compound. Its action is primarily attributed to selective inhibition of the reuptake of serotonin (5-hydroxytryptamine) in the central nervous system. Objectives ; the aims of this study were two-fold: (1) to determine the usefulness for investigation of the transcription profiles in depression patients, and (2) to assess the differences in gene expression profiles between positive response group and negative response groups by fluoxetine treatment. This study included 53 patients with major depression (26 in positive response group with antidepressant treatment, 27 in negative response group with antidepressant treatment), and 53 healthy controls. To examine the difference of gene expression profile in depression patients, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 genes in total. Using 33p-labeled probes, this method provided highly sensitive gene expression profiles including brain receptors, drug metabolism, and cellular signaling. Gene transcription profiles were classified into several categories in accordance with the antidepressant gene-regulation. The gene profiles were significantly up-(22 genes) and down-(16 genes) regulated in the positive response group when compared to the control group. Also, in the negative response group, 35 genes were up-regulated and 8 genes were down-regulated when compared to the control group. Consequently, we demonstrated that radioactive human cDNA microarray is highly likely to be an efficient technology for evaluating the gene regulation of antidepressants, such as selective serotonin-reuptake inhibitors (SSRIs), by using high-throughput biotechnology.

  18. [Primary culture of cat intestinal epithelial cell and construction of its cDNA library].

    Science.gov (United States)

    Ye, L; Gui-Hua, Z; Kun, Y; Hong-Fa, W; Ting, X; Gong-Zhen, L; Wei-Xia, Z; Yong, C

    2017-04-12

    Objective To establish the primary cat intestinal epithelial cells (IECs) culture methods and construct the cDNA library for the following yeast two-hybrid experiment, so as to screen the virulence interaction factors among the final host. Methods The primary cat IECs were cultured by the tissue cultivation and combined digestion with collagenase XI and dispase I separately. Then the cat IECs cultured was identified with the morphological observation and cyto-keratin detection, by using goat anti-cyto-keratin monoclonal antibodies. The mRNA of cat IECs was isolated and used as the template to synthesize the first strand cDNA by SMART™ technology, and then the double-strand cDNAs were acquired by LD-PCR, which were subsequently cloned into the plasmid PGADT7-Rec to construct yeast two-hybrid cDNA library in the yeast strain Y187 by homologous recombination. Matchmaker™ Insert Check PCR was used to detect the size distribution of cDNA fragments after the capacity calculation of the cDNA library. Results The comparison of the two cultivation methods indicated that the combined digestion of collagenase XI and dispase I was more effective than the tissue cultivation. The cat IECs system of continuous culture was established and the cat IECs with high purity were harvested for constructing the yeast two-hybrid cDNA library. The library contained 1.1×10 6 independent clones. The titer was 2.8×10 9 cfu/ml. The size of inserted fragments was among 0.5-2.0 kb. Conclusion The yeast two-hybrid cDNA library of cat IECs meets the requirements of further screen research, and this study lays the foundation of screening the Toxoplasma gondii virulence interaction factors among the cDNA libraries of its final hosts.

  19. cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes.

    OpenAIRE

    Candeliere, G A; Rao, Y; Floh, A; Sandler, S D; Aubin, J E

    1999-01-01

    A cDNA fingerprinting strategy was developed to identify genes based on their differential expression pattern during osteoblast development. Preliminary biological and molecular staging of cDNA pools prepared by global amplification PCR allowed discrim-inating choices to be made in selection of expressed sequence tags (ESTs) to be isolated. Sequencing of selected ESTs confirmed that both known and novel genes can be isolated from any developmental stage of interest, e.g. from primitive progen...

  20. Construction and characteristics of 3-end enriched cDNA library from individual embryos of cattle.

    Science.gov (United States)

    Long, Jian-Er; He, Li-Qiang; Cai, Xia; Ren, Zhao-Rui; Huang, Shu-Zhen; Zeng, Yi-Tao

    2006-11-01

    To analyze stage-specific gene expression profiles of pre-implantation embryos and evaluate potential viability, techniques were adapted to generate 3-end enriched cDNA libraries from individual embryos of cattle based on RT-PCR methodology. The reproducibility of constructing a cDNA library was tested by five independent PCR experiments with specific primers for the presence of several rare genes such as DNMT1 (DNA methylation transferase 1), DNMT2, DNMT3A, Oct-4/3 (octmer-binding transcription factor), IFN-iota, IGF-2r (insulin like growth factor 2 receptor), and the housekeeping genes, H2A and beta-actin. Results indicated repeatability and that a proportion of expressed genes in the cDNA library from an individual embryo was not affected by limited PCR amplification. From the cDNA library, 134 clones were randomly selected for sequencing and showed that structure related elements accounted for 33.5% of transcripts and the energy- and metabolism-related genes were also an important component being 11.9% in the cDNA library. Approximately 14% of genes in the library were functionally unknown including greater than 5% of genes that were likely novel because there was no identity in Genbank. The frequency of structure-related genes such as beta-actin and ribosomal proteins in the cDNA library corresponded to other reports and suggested that the cDNA library constructed by RT-PCR might be proportional to the mRNA populations. The cDNA libraries constructed from different stage embryos will provide a powerful tool to explore novel genes relevant to embryogenesis, determine the profiling of stage-specific gene expression, and evaluate the potential viability of embryos.

  1. CDNA cloning, characterization and expression of an endosperm-specific barley peroxidase

    DEFF Research Database (Denmark)

    Rasmussen, Søren Kjærsgård; Welinder, K.G.; Hejgaard, J.

    1991-01-01

    A barley peroxidase (BP 1) of pI ca. 8.5 and M(r) 37000 has been purified from mature barley grains. Using antibodies towards peroxidase BP 1, a cDNA clone (pcR7) was isolated from cDNA expression library. The nucleotide sequence of pcR7 gave a derived amino acid sequence identical to the 158 C...

  2. Construction of an infectious cDNA clone of foot-and-mouth disease ...

    Indian Academy of Sciences (India)

    A stable, full-length cDNA clone of FMDV type O1BFS 1860 preceded by a bacteriophage T7 polymerase promoter was assembled in a plasmid vector pGEMR-7Zf(–). An ∼8.2 kb PCR product was amplified from the cDNA clone and a full-length RNA was generated from it by in vitro transcription. Transfection of BHK-21 ...

  3. cDNA cloning and mRNA expression of heat shock protein 70 gene ...

    African Journals Online (AJOL)

    In this study, the full-length heat shock protein 70 of Tegillarca granosa was cloned from cDNA library by rapid amplification of cDNA end (RACE). The open reading frame (ORF) of heat shock protein 70 was 1968 bp, and it encoded a protein of 655 amino acids with a predicted molecular weight of 71.48 kDa and an ...

  4. Cloning of cDNA encoding steroid 11β-hydroxylase (P450c11)

    International Nuclear Information System (INIS)

    Chua, S.C.; Szabo, P.; Vitek, A.; Grzeschik, K.H.; John, M.; White, P.C.

    1987-01-01

    The authors have isolated bovine and human adrenal cDNA clones encoding the adrenal cytochrome P-450 specific for 11β-hydroxylation (P450c11). A bovine adrenal cDNA library constructed in the bacteriophage λ vector gt10 was probed with a previously isolated cDNA clone corresponding to part of the 3' untranslated region of the 4.2-kilobase (kb) mRNA encoding P450c11. Several clones with 3.2-kb cDNA inserts were isolated. Sequence analysis showed that they overlapped the original probe by 300 base pairs (bp). Combined cDNA and RNA sequence data demonstrated a continuous open reading frame of 1509 bases. P450c11 is predicted to contain 479 amino acid residues in the mature protein in addition to a 24-residue amino-terminal mitochondrial signal sequence. A bovine clone was used to isolate a homologous clone with a 3.5-kb insert from a human adrenal cDNA library. A region of 1100 bp was 81% homologous to 769 bp of the coding sequence of the bovine cDNA except for a 400-bp segment presumed to be an unprocessed intron. Hybridization of the human cDNA to DNA from a panel of human-rodent somatic cell hybrid lines and in situ hybridization to metaphase spreads of human chromosomes localized the gene to the middle of the long arm of chromosome 8. These data should be useful in developing reagents for heterozygote detection and prenatal diagnosis of 11β-hydroxylase deficiency, the second most frequent cause of congenital adrenal hyperplasia

  5. Tõendite kogumine välisriikides ning välisriigi kohtutele tõendite kogumiseks abi osutamine / Maarja Torga

    Index Scriptorium Estoniae

    Torga, Maarja, 1984-

    2017-01-01

    Rahvusvahelistest kokkulepetest tõendite rahvusvahelisel kogumisel ja tõendite rahvusvahelise kogumise eri režiimidest. Tõendite kogumisest välisriigis Eesti kohtumenetluses ja Eesti kohtute kohustustest välisriigi kohtutele tõendite kogumiseks abi osutamisel

  6. Initial fungal colonizer affects mass loss and fungal community development in Picea abies logs 6 yr after inoculation

    Science.gov (United States)

    Daniel L. Lindner; Rimvydas Vasaitis; Ariana Kubartova; Johan Allmer; Hanna Johannesson; Mark T. Banik; Jan. Stenlid

    2011-01-01

    Picea abies logs were inoculated with Resinicium bicolor, Fomitopsis pinicola or left un-inoculated and placed in an old-growth boreal forest. Mass loss and fungal community data were collected after 6 yr to test whether simplification of the fungal community via inoculation affects mass loss and fungal community development. Three...

  7. Nuevos genes reguladores de la tolerancia a estrés abiótico en Arabidopsis.

    OpenAIRE

    MARTÍNEZ MACÍAS, FÉLIX

    2015-01-01

    Martínez Macías, F. (2015). Nuevos genes reguladores de la tolerancia a estrés abiótico en Arabidopsis [Tesis doctoral no publicada]. Universitat Politècnica de València. doi:10.4995/Thesis/10251/48560. TESIS

  8. CITOGENETICS EFFECTS INDUCED BY THE ASCORBIC ACID TREATMENT OF LARIX DECIDUA MILL. SSP. CARPATICA AND PICEA ABIES (L. KARST

    Directory of Open Access Journals (Sweden)

    Ioana Ieremia

    2006-08-01

    Full Text Available The paper present the influence of ascorbic acid upon the mitotic division of Larix decidua Mill ssp. carpatica and Picea abies (L. Karst. The treatment is applied of two variants, germinated seed in ascorbic acid (variantAand germinated seeds in disttilate water, than treated with ascorbic acid in 3 concentrations (variant B.

  9. Bark beetle Polygraphus proximus: a new aggressive far eastern invader on Abies species in Siberia and European Russia

    Science.gov (United States)

    Yuri Baranchikov; Evgeniy Akulov; Sergey. Astapenko

    2011-01-01

    Polygraphus proximus Brandford (Coleoptera: Scolytidae) is a common feeder on Far Eastern firs: Abies nephrolepis, A. hollophyll, and A. sachalinensis. Its native range occupies northeastern China, Korea, Japan, Kurile and Sakhalin Islands, and the southern part of the Russian Far East (Primorskiy and...

  10. Koolieeliku hirmudega toimetulek kui oma probleemidega toimetuleku õppimine ja lähedaste abi selles / Merle Taimalu

    Index Scriptorium Estoniae

    Taimalu, Merle

    2001-01-01

    Eestis ja Soomes 1993-1994.a. läbi viidud ühisuurimusest laste turvalisuse kohta, kus üheks eesmärgiks oli välja selgitada, kuidas koolieelikud oma hirmudega toime tulevad ja kuivõrd nad saavad ja kasutavad lähedaste täiskasvanute abi

  11. Pezicula neosporulosa sp. nov. (Helotiales, Ascomycota), an endophytic fungus associated with Abies spp. in China and Europe

    NARCIS (Netherlands)

    Yuan, Zhilin; Verkley, G.J.M.

    2015-01-01

    A new species of Pezicula, P. neosporulosa associated with Abies alba in the Netherlands and A. beshanzuensis in China is described, illustrated and compared to its sister species P. sporulosa, P. cinnamomea and P. eucrita. Morphologically, P. neosporulosa is shown to be similar to P. sporulosa both

  12. Age-class differences in shoot photosynthesis and water relations of Fraser fir (Abies fraseri), southern Appalachian Mountains, USA

    Science.gov (United States)

    Keith Reinhardt; Daniel M. Johnson; William K. Smith

    2009-01-01

    Fraser fir (Abies fraseri (Pursh) Poir.) is an endemic tree species found only in refugial mountain-top forests in the southern Appalachian Mountains, USA. Very few studies have investigated the ecophysiology of this species in its natural environment. We measured and compared photosynthetic gas exchange and water relations of understory germinant...

  13. Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression.

    Science.gov (United States)

    Yu, Lin-Hui; Wu, Jie; Zhang, Zi-Sheng; Miao, Zi-Qing; Zhao, Ping-Xia; Wang, Zhen; Xiang, Cheng-Bin

    2017-06-05

    Seed germination is a crucial checkpoint for plant survival under unfavorable environmental conditions. Abscisic acid (ABA) signaling plays a vital role in integrating environmental information to regulate seed germination. It has been well known that MCM1/AGAMOUS/DEFICIENS/SRF (MADS)-box transcription factors are key regulators of seed and flower development in Arabidopsis. However, little is known about their functions in seed germination. Here we report that MADS-box transcription factor AGL21 is a negative regulator of seed germination and post-germination growth by controlling the expression of ABA-INSENSITIVE 5 (ABI5) in Arabidopsis. The AGL21-overexpressing plants were hypersensitive to ABA, salt, and osmotic stresses during seed germination and early post-germination growth, whereas agl21 mutants were less sensitive. We found that AGL21 positively regulated ABI5 expression in seeds. Consistently, genetic analyses showed that AGL21 is epistatic to ABI5 in controlling seed germination. Chromatin immunoprecipitation assays further demonstrated that AGL21 could directly bind to the ABI5 promoter in plant cells. Moreover, we found that AGL21 responded to multiple environmental stresses and plant hormones during seed germination. Taken together, our results suggest that AGL21 acts as a surveillance integrator that incorporates environmental cues and endogenous hormonal signals into ABA signaling to regulate seed germination and early post-germination growth. Copyright © 2017 The Author. Published by Elsevier Inc. All rights reserved.

  14. Trehalose as a good candidate for enriching full-length cDNAs in cDNA library construction.

    Science.gov (United States)

    Chen, Lei; Cao, Lixue; Zhou, Longhai; Jing, Yudong; Chen, Zuozhou; Deng, Cheng; Shen, Yu; Chen, Liangbiao

    2007-01-10

    It has been reported that the disaccharide trehalose is capable of increasing the thermostability and thermoactivity of reverse transcriptase, and therefore improving the length of cDNA synthesis. However, no test has been done on how the disaccharide trehalose performs in the context of the entire cDNA synthesis processes, or whether it can seamlessly integrate into the commercially available cDNA synthesis kit. In this report, we optimized a protocol to incorporate trehalose in the Stratagene's cDNA library construction kit in order to demonstrate great improvement in cDNA's length (average length of 1.8 kb in the trehalose group versus 1.0 kb in the control). Sequence analysis of the cDNA clones showed that the addition of trehalose did not increase the error rate of the RT products but greatly increase the quantity of full-length in cDNA library.

  15. Construction of Infectious cDNA Clone of a Chrysanthemum stunt viroid Korean Isolate

    Science.gov (United States)

    Yoon, Ju-Yeon; Cho, In-Sook; Choi, Gug-Seoun; Choi, Seung-Kook

    2014-01-01

    Chrysanthemum stunt viroid (CSVd), a noncoding infectious RNA molecule, causes seriously economic losses of chrysanthemum for 3 or 4 years after its first infection. Monomeric cDNA clones of CSVd isolate SK1 (CSVd-SK1) were constructed in the plasmids pGEM-T easy vector and pUC19 vector. Linear positive-sense transcripts synthesized in vitro from the full-length monomeric cDNA clones of CSVd-SK1 could infect systemically tomato seedlings and chrysanthemum plants, suggesting that the linear CSVd RNA transcribed from the cDNA clones could be replicated as efficiently as circular CSVd in host species. However, direct inoculation of plasmid cDNA clones containing full-length monomeric cDNA of CSVd-SK1 failed to infect tomato and chrysanthemum and linear negative-sense transcripts from the plasmid DNAs were not infectious in the two plant species. The cDNA sequences of progeny viroid in systemically infected tomato and chrysanthemum showed a few substitutions at a specific nucleotide position, but there were no deletions and insertions in the sequences of the CSVd progeny from tomato and chrysanthemum plants. PMID:25288987

  16. Construction of a T7 Human Lung Cancer cDNA Library

    Directory of Open Access Journals (Sweden)

    Wentao YUE

    2008-10-01

    Full Text Available Background and objective Currently, only a limited numbers of tumor markers for non small lung cancer (NSCLC diagnosis, new biomarker, such as serum autoantibody may improve the early detection of lung cancer. Our objective is construction human lung squamous carcinoma and adenocarcinoma T7 phage display cDNA library from the tissues of NSCLC patients. Methods mRNA was isolated from a pool of total RNA extract from NSCLC tissues obtained from 5 adenocarcinomas and 5 squamous carcinomas, and then mRNA was reverse transcribed into double stranded cDNA. After digestion, the cDNA was inserted into T7Select 10-3 vector. The phage display cDNA library was constructed by package reaction in vitro and plate proliferation. Plaque assay and PCR were used to evaluate the library.Results Two T7 phage display cDNA library were established. Plaque assay show the titer of lung squamas carcinoma library was 1.8×106 pfu, and the adenocarcinoma library was 5×106 pfu. The phage titer of the amplified library were 3.2×1010 pfu/mL and 2.5×1010 pfu/mL. PCR amplification of random plaque show insert ratio were 100% (24/24 in adenocarcinoma library and 95.8% in human lung squamas carcinoma library (23/24. Insert range from 300 bp to 1 500 bp. Conclusion Two phage display cDNA library from NSCLC were constructed.

  17. [Construction of suppression subtracted cDNA library of deltamethrin-resistant Aedes albopictus].

    Science.gov (United States)

    Wu, Jia-hong; Zhao, Tong-yan; Dong, Yan-de

    2006-08-01

    To construct the suppression subtracted cDNA library of deltamethrin-resistant Aedes albopictus. Total RNA was extracted from the deltamethrin-resistant (R-lab) and -sensitive (S-lab) isolates, mRNA was obtained after purification. Double stranded cDNAs were synthesized after reverse transcription. Two subtractions were performed by suppression subtractive hybridization with S-lab as tester and R-lab as driver or S-lab as driver and R-lab as tester. Enriched different expressed cDNA was cloned into pMD18-T vector to construct subtractive libraries. The subtracted cDNA libraries contained 580 and 477 positive clones respectively. The PCR results of 150 clones picked randomly from each library showed that the positive ratio of constructed cDNA libraries was 93%, with a length of cDNA fragments ranged from 150bp to 750bp. The suppression subtracted cDNA library of deltamethrin-resistant Ae. albopictus is constructed.

  18. [Construction of the subtracted cDNA libraries related to artemisinin-resistance of Plasmodium berghei].

    Science.gov (United States)

    Bei, Zhu-chun; Wang, Jing-yan

    2004-06-01

    To construct the subtracted cDNA libraries related to artemisinin-resistance of Plasmodium berghei using suppression subtractive hybridization PCR (SSH PCR). Total RNA was extracted from the artemisinin-sensitive (NS) and artemisinin-resistant (AR) strains of Plasmodium berghei K173. The cDNA synthesis followed the protocol of super SMART cDNA synthesis kit. Taking the NS as driver, AR as tester and reverse, two subtractions were performed by SSH PCR. Enriched different expressed cDNA was cloned into pMD18-T vector to construct subtractive libraries. The subtracted cDNA libraries of NS-AR and AR-NS contained 395 and 506 positive clones respectively. The PCR results of 108 clones picked randomly from each library showed 100 and 104 positive inserts contained in the plasmids respectively, and distributing in 250-2000 bp. The successful construction of the subtracted cDNA libraries related to artemisinin-resistance of P. berghei enable us to identify the different expressed genes involved in the resistance mechanism.

  19. [cDNA library constructing and specific antigen expression of Streptomyces thermohydroscopicus].

    Science.gov (United States)

    Xu, Lei; Wang, Ling-ling; Liu, Shuo; Ling, Yuan; Ma, Lie; Wang, Qun; Zhang, Li-jiao; He, Xiao-yu; Zhao, Ming-jing; Wang, Xiao-ge

    2012-03-01

    To construct a cDNA library from Streptomyces thermohydroscopicus and screen genes with virulence, obtain the recombinant fusion virulence proteins by prokaryotic expression system. The Streptomyces thermohydroscopicus cDNA library was constructed by switching mechanism at 5'end of RNA transcript approach. A total of 1020 clones randomly selected from the cDNA library were sequenced and these expressed sequence tags (EST) were further analyzed for the screen of antigen-specific genes. The two candidate genes were subcloned into expression vector pET-28a. The recombinants were transformed into BL2 and proteins were expressed by the induction of isopropyl-β-D-1-thiogalactopyranoside (IPTG). A high-quality cDNA library from Streptomyces thermohydroscopicus was constructed and a set of 978 valid sequences were obtained. Clustering and assembly of these cDNA sequences resulted in 347 unique genes, among which 2 potential antigen-specific genes were highly allied with outer membrane lipoprotein (51%) and transferring-binding protein B (42%) from Actinobacillus pleuropneumoniae serotype (APP). The open reading frame (ORF) of the two candidate genes are 1554 bp and 726 bp, which coded two peptides with 517 and 241 amino acids, respectively. The molecular weights of the recombinant fusion proteins were 63 000 and 30 000. The cDNA library of Streptomyces thermohydroscopicus reached the quality requirement of gene library. EST database in the library would greatly facilitate further screening of virulence genes.

  20. [Pontoscolex corethrurus (Annelidae: Oligochaeta) soil quality indicator in Eucalyptus grandis (Myrtacea) sites with slash and burn management].

    Science.gov (United States)

    Uribe, Sheila; Huerta, Esperanza; Geissen, Violette; Mendoza, Manuel; Godoy, Roberto; Jarquín, Aarón

    2012-12-01

    Soil burning has been used in agricultural and forestry systems as a fundamental technique to clean the land and add some nutrients to the soil. In addition, earthworms are known to promote various soil functions since they contribute to aeration and organic matter and nutrients availability to other soil organisms. This study evaluated the effects of tropical forest crops management with presence-absence of Eucalyptus grandis on earthworm population in Huimanquillo, Tabasco, Mexico. Three sites (average area of 1-1.5ha each) with different management conditions were considered for soil and earthworm sampling (two depths and six replicates): without vegetation (SV) and recent slash-burned (38 days), forest crops of five years of production of E. grandis (Euc), and secondary vegetation of 15 years (Acah). Soil physico-chemical properties (apparent density, humidity, texture, pH, Ntot, OM, P, K, cationic capacity) were also evaluated, and earthworms were collected at the end of the rainy season (august-october 2007). We found that the sites soil is an acrisol acid, with pH 3.0-4.5 in the first 30cm depth. Organic matter content (OM) and total nitrogen (Ntot) in the recently burned sites were significantly lower (6-8% y 0.19-0.22%, respectively) than in sites with vegetation (OM=9-11%; el Ntot=0.27-0.33%). Only one species (P. corethrurus) was found in all the sampled areas, where most of the individuals were at juvenile stage (80%). The highest densities and biomass were found in Euc. treatment (166.4ind/m2 y 36.8g/m2) followed by Acah (138.7ind/m2 y 19.1g/m2 respectively), while the SV treatment showed of about an 80% reduced earthworm populations when compared to other treatments. Even though 15 years have passed over the secondary vegetation (Acah) still some perturbations were observed as the low abundance of the oligochaeta group. We concluded that the management used to culture E. grandis produces negative effects over the abundance and diversity of earthworms

  1. Photolysis of polycyclic aromatic hydrocarbons adsorbed on spruce [Picea abies (L.) Karst.] needles under sunlight irradiation

    International Nuclear Information System (INIS)

    Niu Junfeng; Chen Jingwen; Martens, D.; Quan Xie; Yang Fenglin; Kettrup, A.; Schramm, K.-W.

    2003-01-01

    Photolysis of PAHs on surfaces may determine their ultimate fate in the environment. - Photolysis of polycyclic aromatic hydrocarbons (PAHs) sorbed on surfaces of spruce [Picea abies (L.) Karst.] needles under sunlight irradiation was investigated. PAHs were produced by combustion of polyvinyl chloride (PVC), wood, high-density polyethylene (HDPE), and styrene in a stove. The factors of sunlight irradiation on the surfaces of spruce needles were taken into consideration when investigating the kinetic parameters. The photolysis of the 18 PAHs under study follows first-order kinetics. The photolysis half-lives range from 15 h for dibenzo(a,h)anthracene to 75 h for phenanthrene. Photolysis of some PAHs on surfaces of spruce needles may play an important role on the fate of PAHs in the environment

  2. Annual variation in pollen contamination and outcrossing in a Picea abies seed orchard

    Energy Technology Data Exchange (ETDEWEB)

    Pakkanen, Anne [Finnish Forest Research Inst., Laeyliaeinen (Finland). Haapastensyrjae Breeding Station; Nikkanen, Teijo [Finnish Forest Research Inst., Punkaharju (Finland). Punkaharju Research Station; Pulkkinen, Pertti [Helsinki Univ. (Finland). Dept. of Plant Biology

    2000-07-01

    A mature Norway spruce [Picea abies (L.) Karst.] seed orchard, established in southern Finland with 67 clones from northern Finland, was analysed in three different years in order to estimate the pollen contamination ratios. Allozyme-based paternity analysis revealed that the contamination rate was high, 69-71%, and did not differ between the years studied. It appears that, in areas where spruce is the dominant tree species, the contamination rate will be very high even in mature seed orchards. However, the contamination rate in the thinned parts of the orchard was significantly lower than that in the unthinned parts in two of the three years studied. The outcrossing rate was also high, 96 - 100% in all years, even though the ramets of each clone were planted using a clonal-row design, and there were no significant differences in the outcrossing rates between the different parts of the orchard.

  3. Biochemical Content of Cambium of Abies nephrolepis Eaten by Bears on the Far East of Russia

    Directory of Open Access Journals (Sweden)

    I. V. Seryodkin

    2017-01-01

    Full Text Available The peculiarity of bears behavior of stripping of bark is typical for all species. We have described the damage to trees, by Asiatic black bear (Ursus thibetanus and brown bear (U. arctos in Primorsky Krai and by brown bears on the Sakhalin Island during 1998–2015. In this study, we studied the damaged bark of the tree only in cases where it was clear that part of the cambium was eaten by bears. Cambium of species Abies nephrolepis is the most preferred for bear consumption in Primorsky Krai. We distinguished very large seasonal fluctuations in the amount of its consumption. The greatest interest of bears in this kind of food is in the summer time. We have analyzed the composition of the cambium of A. nephrolepis. These results suggest that the important purpose of the use of this kind of food is to restore and maintain the normal functioning of the intestines.

  4. Growing trees on completed sanitary landfills. [Nyssa sylvatica, Picea abies, Ginkgo biloba

    Energy Technology Data Exchange (ETDEWEB)

    Leone, I.A.; Gilman, E.F.; Flower, F.B.

    1983-01-01

    A 10-year old completed landfill in New Jersey consisting of 9 m (depth) of refuse covered with 15-25 cm of soil was cleared of debris and vegetation and covered with 30 cm of subsoil and 15-25 cm of topsoil. Nineteen coniferous and broadleaved species were planted on the landfill and on a control site in 1975, and trees were maintained and growth and condition monitored over 4 years. On the basis of shoot length and stem area increase, the most successful of the surviving trees were Nyssa sylvatica, Picea abies and Ginkgo biloba, in decreasing order of tolerance. Tolerance of landfill conditions appeared to be greatest in those species with low water requirements, a slow growth rate, high acid tolerance and a shallow root system. (Refs. 11).

  5. In vitro testing of defense reactions in zygotic and somatic embryos of Abies numidica

    Directory of Open Access Journals (Sweden)

    Jiří Hřib

    2011-01-01

    Full Text Available Defense of desiccated cotyledonary somatic embryos and mature zygotic embryos of Abies numidica was tested in vitro by dual cultures with tester, fungus Phaeolus schweinitzii. Both types of embryos expressed defense reactions manifested by inhibited growth of fungal tester towards the embryos. Mycelial growth was described by logistic sigmoid growth model with a single asymptote. Mutual comparisons of mycelial growth in presence of zygotic and somatic embryos showed significant differences in parameters of mycelium growth curves towards the embryos. Larger defense reactions were observed in zygotic embryos relative to somatic embryos and unlimited control cultivations without embryo. The possible role of auxin in the defense response of plant embryos is discussed.

  6. Antibacterial effects of home-made resin salve from Norway spruce (Picea abies).

    Science.gov (United States)

    Rautio, M; Sipponen, A; Peltola, R; Lohi, J; Jokinen, J J; Papp, A; Carlson, P; Sipponen, P

    2007-04-01

    Resin salve made from Norway spruce (Picea abies) is traditionally used in folk medicine to heal skin ulcers and infected wounds. Its antimicrobial properties were studied against certain human bacteria important in infected skin wounds. The sensitivity of the resin against Gram-positive and Gram-negative bacteria was studied in vitro by methods that are routinely used in microbiology laboratories. The resin salve exhibited a bacteriostatic effect against all tested Gram-positive bacteria but only against Proteus vulgaris of the Gram-negative bacteria. Interestingly, the resin inhibited the growth of bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococcus (VRE), both on agar plates and in culture media. The study demonstrated antimicrobial activity of the resin salve and provided objective evidence of its antimicrobial properties. It gives some explanations why the traditional use of home-made resin salve from Norway spruce is experienced as being effective in the treatment of infected skin ulcers.

  7. Response of Abies alba embryonal-suspensor mass to various carbohydrate treatments.

    Science.gov (United States)

    Schuller, A; Reuther, G

    1993-02-01

    Cultures of embryonal-suspensor mass of European silver fir (Abies alba) were screened for their response to various sucrose concentrations (29-175 mM) and to equimolar (100 or 200 mM) concentrations of glucose, fructose, galactose, sucrose, lactose and soluble starch. The higher concentrations were combined with 3.78 μM abscisic acid. While fructose was ineffective in promoting any somatic embryo maturation glucose, sucrose and galactose promoted early maturation stages. Somatic embryo development up to the torpedo stage was observed only on media with soluble starch or lactose. Lactose was superior to other carbohydrate treatments and led to more synchronized maturation and more normally structured embryos. In comparison with the pronounced carbohydrate effect abscisic acid at this concentration proved to be of less importance. A sequence of culture steps was established in order to stimulate differentiation and maturation.

  8. [Genetic variation and differentiation of Abies alba Mill. populations from Ukrainian Carpathians].

    Science.gov (United States)

    Korshikov, I I; Pirko, N N; Pirko, Ya V

    2005-03-01

    Using electrophoretic analysis of 11 enzyme systems, we studied the genetic structure and differentiation of eight natural populations of silver fir Abies alba Mill. in the Ukrainian Carpathians. Of 24 isozyme loci identified, 66.8% proved to be polymorphic. The mean numbers of alleles and genotypes per locus in the populations were respectively 3.1 and 4.5. Each A. alba tree was on average heterozygous at 15.9% of genes. In six populations, the genotypic distribution for all of the loci examined corresponded to Hardy-Weinberg proportions. The populations studied had low levels of subdivision (F(ST) = 0.018; GST = 0.019) and differentiation. Nei's genetic distances between the A. alba populations in the region ranged from 0.002 to 0.009, being on average 0.006.

  9. Sclerification in the bark tissues of common fir (Abies alba Mill.

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    Sławomir Janakowski

    2014-01-01

    Full Text Available The sclerification process in bark tissues of common fir (Abies alba Mill. has been described. The sclerification begins in 3 years old stems. Sclereids differentiate from cortical parenchyma cells and from secondary phloem parenchyma cells that do not contain phenolic deposits. The first single sclereids are formed at the interface of the cortex and nonfunctional phloem. Hereafter, a continuous layer of them is formed. Later, new sclereid layers are formed successively in nonfunctional secondary phloem and cortex. The consecutive layers are separated tangentially by phloem parenchyma cells, that accumulate large amounts of phenolic substances, and by compressed phloem cells. Laterally they are separated by phloem rays that except of some dislocations are continuous. Structural net of the cortical phloem ray cells and phloem parenchyma delineates the areas where the formations of sclereid layers occurs in nonfunctional secondary phloem. Older cortex contains more sclereid layers and the time period of their formation extends continuously.

  10. Mycocoenology in Abies alba Miller woods of central-southern Tuscany (Italy

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    Angela Laganà

    2014-01-01

    Full Text Available Numerous reports indicate that fir woods in central and northern Europe have recently been damaged by increasing pollution. It has been demonstrated that fungi can be good bioindicators of forest health status. In polluted areas the production of fruit bodies generally declines and the fungal biodiversity, especially of symbiotic species, is reduced. Here we report the results of a survey of the fungal and plant communities in woods of Abies alba Miller in central-southern Tuscany, already studied in the past. Certain changes were observed in the study areas, but they seem more likely to be due to other factors than pollution, such as the weather conditions, the age and natural evolution of the forests towards their climax.

  11. Differentiation of somatic embryos from protoplasts isolated from embryogenic suspension cultures of Abies alba L.

    Science.gov (United States)

    Hartmann, S; Lang, H; Reuther, G

    1992-10-01

    Embryogenic suspension cultures of Abies alba were established using an embryogenic suspensor mass culture originating from the zygotic embryo in immature seed explants (Schuller et al. 1989). Protoplasts were isolated from the suspension material. The protoplasts were immobilized in alginate layers in order to follow the development of single protoplasts. During the first days of protoplast culture a modified Kao and Michayluk (1975) medium proved to be necessary for subsequent divisions. The formation of proembryos succeeded within 2-3 weeks when subcultured with a modified Schenk and Hildebrandt (1972) liquid medium. Light, enhanced sugar concentration, and the addition of abscisic acid led to the formation of slightly green "torpedo"-shaped somatic embryos after 6-8 weeks from protoplast isolation.

  12. An experimental study on the effects of exhaust gas on spruce (Picea abies L. Karst.)

    Energy Technology Data Exchange (ETDEWEB)

    Hautala, E.L.; Holopainen, J.; Kaerenlampi, L. [Kuopio Univ. (Finland). Dept. of Ecology and Environmental Science; Surakka, J.; Ruuskanen, J. [Kuopio Univ. (Finland). Dept. of Environmental Sciences

    1995-12-31

    Motor vehicle exhausts are significant contributors to air pollution. Besides fine particles and inorganic gases, like CO, SO{sub 2} and NO{sub x}, exhaust gas contains a large group of aromatic hydrocarbon compounds, many of which are phytotoxic. In field studies, exhausts are found to have both direct and indirect harmful effects on roadside plants. However, only few experimental studies have been made about the effects of exhaust gas emissions on coniferous trees. The aim of this study was to survey the effects of exhausts on spruce (Picea abies L. Karst.) in standardized conditions. The concentrations of major exhaust gas components in the chamber atmosphere were detected simultaneously. The effects of exhaust on epistomatal waxes of first-year spruce needles are described. (author)

  13. The work of Ernst H. Gombrich on the Aby M. Warburg fragments

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    Katia Mazzucco

    2011-12-01

    Full Text Available On January 1, 1936, Ernst Gombrich arrived in London from Austria. His task was to reorganize the last of Warburg’s fragments and, in particular, to revise what was left of the incomplete mnemosyne atlas. The goal of this study and inventory was to collate Warburg’s uncompleted studies with the purpose of putting together an English publication of his collected work. In 1970, the only result of this publishing endeavour was included in the pages of the famous Aby Warburg. An intellectual biography. The history of the intellectual, as well as critical, relationship between Gombrich and Warburg is, then, a posthumous one that was started on British soil. Further marking this distance are certain material and non-material aspects of Gombrich’s work in London, including his relationship with other warburgian scholars.

  14. Sobre el caso Aby Warburg : La cura por el símbolo

    OpenAIRE

    Erbetta, Anahí E.; Morete, Amalia

    2015-01-01

    En el presente trabajo nos ocuparemos del caso del historiador de arte alemán Aby Warburg (1886-1929), reconocido por el estudio de la historia cultural a través de las artes visuales. Retiene nuestra atención desde una perspectiva psicoanalítica el desencadenamiento de una psicosis clínica a la edad de 52 años. Es internado en la clínica Bellevue, en Kreuzlingen, dirigida por el Dr. Binswanger, desde el año 1921 al año 1924. Si bien el motivo de internación reside en el riesgo de vida en que...

  15. Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla Organogênese e transformação genética transiente do híbrido Eucalyptus grandis × Eucalyptus urophylla

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    Giovana Bomfim de Alcantara

    2011-04-01

    Full Text Available The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium every five days. This study also aimed at evaluating factors affecting the genetic transformation of leaf explants with the uidA gene, via co-cultivation with Agrobacterium tumefaciens, such as the pre-culture of the explants on a specific medium, the duration of their co-culture with the bacteria and the addition of acetosyringone to the culture media. The best conditions for the expression of the uidA gene were two days of pre-culture of the leaf tissues, three days of co-culture with the bacteria and the addition of acetosyringone in pre- and co-culture media.O híbrido Eucalyptus grandis x Eucalyptus urophylla apresenta alta produtividade e potencial para indústrias de papel, celulose e fibras. A organogênese de explantes foliares de dois clones de E. grandis × E. urophylla foi estudada para verificar fatores como tempo de repicagem das plântulas matrizes em meio de multiplicação; tipo de explantes, folhas inteiras e de meias-folhas (porções basais e apicais e dos dias que os explantes foliares permaneceram em meio de regeneração. Foram observadas diferenças na capacidade organogênica dos dois clones testados. A parte basal das folhas, coletadas de brotações repicadas a cada 17

  16. Influência da morfologia das fibras na usinabilidade da madeira de Eucalyptus grandis Hill ex. Maiden Influence of fiber morphology on the wood workability of Eucalyptus grandis Hill ex. Maiden

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    José Reinaldo Moreira da Silva

    2005-06-01

    Full Text Available As dificuldades quanto ao uso da madeira do eucalipto, na forma sólida, estão baseadas na falta de conhecimentos de como trabalhar corretamente esse material. É preciso conhecer a estrutura da madeira e os parâmetros de usinagem para entender suas relações, que proporcionam os bons resultados em qualidade. O presente trabalho visou verificar as variações no sentido medula-casca de diferentes operações de usinagem e das dimensões das fibras da madeira de Eucalyptus grandis Hill ex. Maiden. A madeira foi proveniente de plantio comercial da Klabin Fabricadora de Papel e Celulose S.A., com 25 anos de idade. Os dados foram avaliados através de análises de variância e testes de médias. Além disso, realizaram-se as correlações de Pearson entre as operações de usinagem e as dimensões das fibras. Foram obtidos diferentes modelos de variação da medula para a casca. Nas operações de usinagem, pôde-se concluir que as condições de corte foram insuficientes para apresentar melhores qualidades das superfícies, podendo detacar as baixíssimas velocidades de corte, que produziram pré-clivagem. A madeira de Eucalyptus grandis apresentou resultados satisfatórios, mas inferiores aos do mogno e da imbúia. A utilização de operações como a moldura no topo (corte 90º-90º, moldura axial ";parada"; (corte 90º-0º e perfilagem axial sinuosa com faca plana (corte 90º-0º, contra as fibras permitem apreciar a usinabilidade da madeira, pois esta é usinada sob drásticas condições, podendo mostrar seu verdadeiro potencial.The difficulties to the use of eucalypt as solid wood are based on the lack of knowledge about the process of how to work correctly this material. It is necessary to know both the wood structure and wood workability parameters to better understand their relationships. Thus, this research aimed to identify the variation of wood machining property and the fiber morphology from pith to bark in 25-year-old Eucalyptus

  17. In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium=Efeitos do boro e cálcio na organogênese in vitro de Eucalyptus grandis

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    Gilvano Ebling Brondani

    2012-10-01

    Full Text Available The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The concentrations of boron and calcium in the culture medium influenced the in vitro organogenic control of Eucalyptus grandis. Reduced combinations of boron and calcium induced callus formation and dry matter accumulation in the explants. A boron concentration of 100% (1.10 mg L-1 combined with 100% (119.950 mg L-1 and 200% (239.900 mg L-1 of calcium, and 200% (2.20 mg L-1 of boron combined with 100% (119.950 mg L-1 of calcium allowed the induction of well-developed buds, which can be used for the regeneration of micro-plants.A organogênese in vitro de espécies lenhosas caracteriza-se como fator fundamental para a obtenção de mudas com interesse comercial, gerando aplicações práticas na área do melhoramento florestal. Adicionalmente, a nutrição mineral desempenha papel importante nas respostas de indução organogênica. Objetivou-se avaliar o efeito do boro e cálcio na organogênese de segmentos nodais oriundos de plântulas de Eucalyptus grandis germinadas in vitro. Segmentos nodais com 45 dias após a germinação foram utilizados como explantes. Os explantes foram submetidos a combinações de boro e cálcio em meio de cultura MS, visando induzir diferentes respostas organogênicas. Ao

  18. Efeito de quatro tipos de mel na longevidade e reprodução de Catolaccus grandis (Hymenoptera:pteromalidae Effect of four types of honey on longevity and reproduction of Catolaccus grandis (Hymenoptera:Pteromalidae

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    Paulo Alves Wanderley

    2004-08-01

    Full Text Available O trabalho teve como objetivo conhecer o melhor tipo de mel em relação à longevidade e fertilidade do parasitóide do bicudo-do-algodoeiro Catolaccus grandis. Adultos recém-emergidos foram transferidos para recipientes plásticos de 500mL, adaptados com tubo para fornecer água e umidade para os insetos. Um casal do parasitóide foi mantido em cada recipiente em câmara climatizada a 25±1°C, UR = 70±10% e fotoperíodo de 14h. O trabalho constou de 4 tratamentos: mel de flor de laranjeira; mel de flores de plantas silvestres; mel de cana-de-açúcar (todos produzidos por Apis mellifera e mel de flores silvestres produzido por abelha Jataí (Tetragonistica angustula, com 15 repetições cada. Adultos recém-emergidos do parasitóide receberam cinco larvas de Euscepes postfasciatus encapsuladas em parafilm. Três gotículas de mel foram colocadas sobre o parafilm. Calculou-se a longevidade de machos e fêmeas, número de ovos dia-1 fêmea-1 e número total de ovos fêmea-1 e construíram-se as tabelas de fertilidade. As melhores dietas para alimentar adultos de C. grandis foram o mel silvestre e o de laranjeira. A melhor fecundidade foi observada na dieta de mel de laranjeira (101,60 ovos fêmea-1 e os melhores resultados para aumento reprodutivo e populacional deste parasitóide foram obtidos com mel de laranja e com mel silvestre.The aim of this work was to investigate the best type of honey in relation to longevity and life fertility of Catolaccus grandis. Recent emerged adults were transferred to 500ml plastic recipients adapted to supply water and moisture to insects. A couple of parasite was kept for each recipient in climatic chamber at 25±1°C, RH = 70±10% and photofase 14h. The work had four treatments: orange, wild plant species honey, Jatai Tetragonistica angustula and sugar cane honey with fifteen replications each. Recent emerged adults received five Euscepes postfasciatus larvae, which were encapsulated in parafilm. Three

  19. Soil attributes and wood quality for pulp production in plantations of Eucalyptus grandis clone Atributos do solo e qualidade da madeira para produção de celulose em plantações clonais de Eucalyptus grandis

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    José Luiz Gava

    2008-01-01

    Full Text Available The soil attributes can affect the wood quality of eucalypt, which may result in considerable effect on cellulose production. This study evaluated the effect of different physical and chemical soil attributes on wood quality of Eucalyptus grandis for cellulose production. Five sites were selected at the Western Plateau of the State of São Paulo, planted with one clone of Eucalyptus grandis, with ages ranging between 6.5 and 7.0 years. Four soil types, with texture ranging from sandy to very clayey were found. At each site, three experimental plots were allocated with 100 trees each. Trees representative of each class frequency of diameter at breast height were harvested. Their biomass and wood components were characterized. The wood productivity and quality was affected by physical attributes of soil, mainly clay content, which is directly related to the amount of available water. Basic wood density did not changed at different soil types. Total lignin content decreased and holocellulose content exponentially increased as soil clay content increased (until about 350 to 400 g kg-1 of clay. The wood extractives content was not affected by soil attributes. Screened cellulose yield exponentially increased with soil clay content.Os atributos edáficos podem afetar a qualidade da madeira de eucalipto, o que pode resultar em considerável efeito sobre a produção de celulose. Este trabalho teve como objetivo avaliar o efeito de atributos físicos e químicos do solo na qualidade da madeira de Eucalyptus grandis usada para polpação celulósica. Foram selecionadas cinco áreas no planalto ocidental do Estado de São Paulo, Brasil, plantadas com um mesmo clone de Eucalyptus grandis, com idades variando entre 6,5 e 7,0 anos de idade. Quatro classes de solo, com textura arenosa a muito argilosa, foram encontradas. Em cada uma das cinco áreas, foram demarcadas, aleatoriamente, 3 parcelas com 100 plantas cada. Em cada parcela, foram colhidas

  20. Genetic diversity of Norway spruce [Picea abies (L. Karst.] in Romanian Carpathians

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    Raul Gheorghe Radu

    2014-07-01

    Full Text Available The genetic diversity of Romanian most important coniferous tree species, the Norway spruce, was estimated by means of allozyme markers. A total of 695 adult trees sampled from eleven populations grouped in six mountainous areas in the Romanian Carpathians were analyzed. In three metapopulations (Maramureş, Postăvar and Parâng, to evaluate the influence of altitudinal gradient on genetic diversity, samples were collected from populations located at high and low altitude. At other location (ApuseniMountains we compared the narrow-crown biotype (Picea abies var. columnaris and the pyramidal crown biotype (Picea abies var. pyramidalis and explored the genetic structure of peat bog ecotype. By analyzing 7 enzyme systems and 12 enzyme coding loci, a total of 38 allelic variants have been detected. The mean value of polymorphic loci for the six sites was 86.1%, ranging between 83.3% and 91.7% and the mean expected heterozygosity was 0.115, resulting in a moderate level of genetic diversity. The highest genetic diversity (He = 0.134 was found in the narrow-crown spruce population. Apuseni metapopulation showed the highest genetic diversity (He = 0.125, being the most valuable for conservation of genetic resources. The small value of fixation index (FST = 0.009 indicates a low genetic differentiation between the six sites and AMOVA test revealed a very high level of genetic diversity within population (99%. Comparative analysis of genetic parameters showed small differences between high and low altitude populations at each site, probably due to the neutral character of the markers analyzed and the effect of gene flow between gradiental populations.

  1. Radical Scavenging Activity of the Essential Oil of Silver Fir (Abies alba).

    Science.gov (United States)

    Yang, Seun-Ah; Jeon, Sang-Kyung; Lee, Eun-Jung; Im, Nam-Kyung; Jhee, Kwang-Hwan; Lee, Sam-Pin; Lee, In-Seon

    2009-05-01

    The essential oil of silver fir (Abies alba) is known to help respiratory system and have easing and soothing effect for muscle. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities of silver fir (Abies alba) essential oil. The composition of the oil was analyzed by GC-MS and bornyl acetate (30.31%), camphene (19.81%), 3-carene (13.85%), tricyclene (12.90%), dl-limonene (7.50%), alpha-pinene (2.87%), caryophyllene (2.18%), beta-phellandrene (2.13%), borneol (1.74%), bicyclo[2.2.1]hept-2-ene,2,3-dimethyl (1.64%) and alpha-terpinene (1.24%) were the major components in the oil. The results tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay indicated that the oil showed no cytotoxic effect, at concentrations of 1 and 5%, for as long as 24 and 3 h, respectively. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction (RC(50)) against DPPH radicals (2.7 +/- 0.63%) was lower than ABTS radicals (8.5 +/- 0.27%). The antibacterial activity of the oil was also evaluated using disc diffusion method against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Acinetobacter baumannii, Escherichia coli, and Vibrio parahaemolyticcus. The oil exhibited no antibacterial activity against all the bacterial strains tested except S. aureus of mild activity.

  2. Ecological requirements of Abies alba in the French Alps derived from dendro-ecological analysis

    Energy Technology Data Exchange (ETDEWEB)

    Rolland, C.; Michalet, R.; Desplanque, C.; Petetin, A.; Aime, S. [Univ. Joseph Fourier, Grenoble (France). Centre de Biologie Alpine

    1999-06-01

    We used dendro-ecological techniques to investigate fundamental relationships between climate and growth of Abies alba (silver fir) in eastern France. Seven Abies forests in the Trieves region of the French Alps were chosen to represent a wide range of ecological conditions based on the results of previous forest vegetation surveys. In each forest, four trees were sampled in each of five different stands with two cores per tree. These 280 cores were studied using two separate dendro-ecological methods: the pointer years method (based on extreme growth events), and correlation functions between tree ring-widths and monthly climatic data. Data from 11 meteorological stations were combined to provide a regional analysis of precipitation and minimum and maximum temperatures. The two dendro-ecological methods appear to be complementary, as the first technique emphasizes common and low intensity linear correlations between ring-widths and climatic variations, and the second method emphasizes extreme and unusual climatic events such as exceptionally cold or dry years. Across all sites, drought in the previous year was consistently correlated with a low growth rate; however, other climatic variables varied substantially among sites. For example, drought in the current year reduced growth more in the low elevation sites than in the high elevation sites and severe winter frost reduced growth the most in the high altitude sites and the driest site. Moreover, certain growth responses are better correlated with the age of the stands, the canopy closure and the floristic composition of the community than the abiotic factors, emphasizing the value of dendro-ecological sampling based on phytosociological units 63 refs, 4 figs, 1 tab

  3. Assessment of genetic diversity of silver fir (Abies alba Mill. in Serbia using SSR markers

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    Popović Vladan

    2017-01-01

    Full Text Available The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill. in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubočica Bare and Stara Planina; Dubočica Bare and Tara and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara. A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests. [[Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31070: The developments of technological procedures in forestry in order to attain an optimal forest cover percentage

  4. Effect of light regime on the natural regeneration of silver fir (Abies alba, Calabria, Southern Italy

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    Mercurio R

    2008-06-01

    Full Text Available Aim of this study is to analyse the effect of light environment on dynamics of natural regeneration of silver fir. The study areas were located in natural silver fir (Abies alba Mill. stands of Serra San Bruno Forests (Archiforo in the Calabrian Apennine. Measurements of the Photosynthetically Active Radiation (PAR - radiation in the 0.4-0.7 µm waveband were collected with a ceptometer (AccuPAR Decagon Devices, USA. PAR was measured monthly during the growing season. Measurements were taken: under canopy, inside natural gaps and in correspondence of escarps along forestry roads. After each measurement for each sampling point the PAR transmittance was calculated. Seedlings density was estimated in each position. Each of these seedlings was identified by species, total height was measured and the age was visually determined. Seedlings were distributed in three classes: (a class 0: to natural regeneration absence and height 10 seedlings m-2 and height between 0.30 and 1 m; (c class 2: to density > 10 seedlings m-2 and height > 1 m. Level of PAR transmittance was higher inside the gaps where a good seedlings density was observed. Further seedling density was higher in correspondence of escarps. Under canopy, seedlings were absent due to low levels of light (1.3 < T < 3.7. The results showed a diverse ecological behaviour to light environment of silver fir of Serra San Bruno compared with central-northern Abies alba population. Some comments about silvicultural treatment of silver fir stand in the Calabrian Apennine are provided, according to dynamics of natural regeneration.

  5. Compósitos de partículas de madeira de Eucalyptus grandis, polipropileno e polietileno de alta e baixa densidades Composites of Eucalyptus grandis wood, polypropylene, and high and low-density polyethylene particles

    Directory of Open Access Journals (Sweden)

    Emerson Gomes Milagres

    2006-06-01

    Full Text Available Este trabalho teve como objetivo determinar as propriedades de painéis fabricados com mistura de partículas de madeira de Eucalyptus grandis, polietileno de alta densidade, polietileno de baixa densidade e polipropileno. Empregaram-se duas formulações adesivas (uréia-formaldeído e uréia-formaldeído contendo 0,5% de epóxi. De modo geral, as propriedades dos painéis foram afetadas pela composição das partículas. Os painéis com melhores propriedades foram fabricados com 75% de partículas de madeira e 25% de partículas de polietileno de alta densidade. A adição de epóxi ao adesivo uréico aumentou os valores do módulo de ruptura, dureza Janka, e reduziu o inchamento, em espessura, de alguns painéis. As propriedades mecânicas da maioria dos painéis, exceto o módulo de elasticidade, ultrapassaram os valores mínimos estabelecidos na norma ANSI/A1-208/93.The objective of this work was to establish the properties of particleboards fabricated with blends of Eucalyptus grandis, low-density polyethylene, high-density polyethylene and polypropylene particles. Two adhesives formulations were used (urea-formaldehyde and urea-formaldehyde plus 0,5% of epoxy adhesive. Panel properties were affected by particle composition. Panels with best properties were fabricated with 75% of wood particles and 25% of high density polypropylene. The addition of epoxy to the urea-formaldehyde adhesive improved of the values of modulus of rupture, hardness and reduced the amount of thickness swelling. Except for the modulus of elasticity, board properties meet grade M-2 ANSI/A1-208/93 requirements.

  6. Efficacy Testing of H56 cDNA Tattoo Immunization against Tuberculosis in a Mouse Model.

    Science.gov (United States)

    Platteel, Anouk C M; Nieuwenhuizen, Natalie E; Domaszewska, Teresa; Schürer, Stefanie; Zedler, Ulrike; Brinkmann, Volker; Sijts, Alice J A M; Kaufmann, Stefan H E

    2017-01-01

    Tuberculosis (TB), caused by Mycobacterium tuberculosis ( Mtb ), remains a global threat. The only approved vaccine against TB, Mycobacterium bovis bacillus Calmette-Guérin (BCG), provides insufficient protection and, being a live vaccine, can cause disseminated disease in immunocompromised individuals. Previously, we found that intradermal cDNA tattoo immunization with cDNA of tetanus toxoid fragment C domain 1 fused to cDNA of the fusion protein H56, comprising the Mtb antigens Ag85B, ESAT-6, and Rv2660c, induced antigen-specific CD8 + T cell responses in vivo . As cDNA tattoo immunization would be safer than a live vaccine in immunocompromised patients, we tested the protective efficacy of intradermal tattoo immunization against TB with H56 cDNA, as well as with H56_E, a construct optimized for epitope processing in a mouse model. As Mtb antigens can be used in combination with BCG to boost immune responses, we also tested the protective efficacy of heterologous prime-boost, using dermal tattoo immunization with H56_E cDNA to boost BCG immunization in mice. Dermal H56 and H56_E cDNA immunization induced H56-specific CD4 + and CD8 + T cell responses and Ag85B-specific IgG antibodies, but did not reduce bacterial loads, although immunization with H56_E ameliorated lung pathology. Both subcutaneous and intradermal immunization with BCG resulted in broad cellular immune responses, with increased frequencies of CD4 + T effector memory cells, T follicular helper cells, and germinal center B cells, and resulted in reduced bacterial loads and lung pathology. Heterologous vaccination with BCG/H56_E cDNA induced increased H56-specific CD4 + and CD8 + T cell cytokine responses compared to vaccination with BCG alone, and lung pathology was significantly decreased in BCG/H56_E cDNA immunized mice compared to unvaccinated controls. However, bacterial loads were not decreased after heterologous vaccination compared to BCG alone. CD4 + T cells responding to Ag85B- and ESAT-6

  7. Gladiolus hybridus ABSCISIC ACID INSENSITIVE 5 (GhABI5) is an important transcription factor in ABA signaling that can enhance Gladiolus corm dormancy and Arabidopsis seed dormancy

    Science.gov (United States)

    Wu, Jian; Seng, Shanshan; Sui, Juanjuan; Vonapartis, Eliana; Luo, Xian; Gong, Benhe; Liu, Chen; Wu, Chenyu; Liu, Chao; Zhang, Fengqin; He, Junna; Yi, Mingfang

    2015-01-01

    The phytohormone abscisic acid (ABA) regulates plant development and is crucial for abiotic stress response. In this study, cold storage contributes to reducing endogenous ABA content, resulting in dormancy breaking of Gladiolus. The ABA inhibitor fluridone also promotes germination, suggesting that ABA is an important hormone that regulates corm dormancy. Here, we report the identification and functional characterization of the Gladiolus ABI5 homolog (GhABI5), which is a basic leucine zipper motif transcriptional factor (TF). GhABI5 is expressed in dormant vegetative organs (corm, cormel, and stolon) as well as in reproductive organs (stamen), and it is up-regulated by ABA or drought. Complementation analysis reveals that GhABI5 rescues the ABA insensitivity of abi5-3 during seed germination and induces the expression of downstream ABA response genes in Arabidopsis thaliana (EM1, EM6, and RD29B). Down-regulation of GhABI5 in dormant cormels via virus induced gene silence promotes sprouting and reduces the expression of downstream genes (GhLEA and GhRD29B). The results of this study reveal that GhABI5 regulates bud dormancy (vegetative organ) in Gladiolus in addition to its well-studied function in Arabidopsis seeds (reproductive organ). PMID:26579187

  8. SITE INDEX CURVES AND HYPSOMETRIC RELATIONSHIP FOR Eucalyptus grandis PLANTATIONS FOR THE CAMPOS GERAIS REGION, PARANA STATE

    Directory of Open Access Journals (Sweden)

    Fabiane Aparecida de Souza Retslaff

    2015-06-01

    Full Text Available The study aimed to fit mathematical models for the construction of Site Index curves and to estimate heights at different ages for Eucalyptus grandis in the Campos Gerais region, Parana State. The data used to fit the models came from permanent, temporary plots and pre-harvesting inventory, covering ages from 2.5 to 26.5 years. Several models were tested to represent the sites and the hypsometric relationship. The Site Index curves were constructed by the guide-curve method. For the Site Index, the Chapman-Richards model showed the best fit and precision statistics, generating 5 Site Index curves (range of 5 m with the Chapman-Richards model. The four hypsometric models tested showed satisfactory performance and similar statistics and the inclusion of the variables dominant height or site index did not substantially improve the goodness of fit statistics, but the residues were more homogeneous and closer to zero.

  9. EFFECT OF THINNING AND FERTILIZATION ON Eucalyptus grandis Hill ex-Maiden PITH DISPLACEMENT AND LOG END SPLITTlNG

    Directory of Open Access Journals (Sweden)

    Israel Luiz de Lima

    2007-06-01

    Full Text Available The aim of this study was to analyse the influence of thinning and fertilization on some log properties which influence sawn wood yield in a 21 year-old grown Eucalyptus grandis stand. Three selective thinning intensities (37, 50 and 75%, presence or absence of fertilizers on the beginning of the thinning, two diameter classes and two logs taken from two different vertical positions were taken as the study factors. The influence of the factors and their combination on tree growth stresses were indirectly evaluate through log end splitting and pith displacement in relation to its normal central position. Thinning, fertilization, diameter class and log position on the tree height did not have significant effect on pith displacement. The logs from fertilized plots presented higher log end splitting index than others.

  10. CANONICAL CORRELATION OF PHYSICAL AND CHEMICAL CHARACTERISTICS OF THE WOOD OF Eucalyptus grandis AND Eucalyptus saligna CLONES

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Trugilho

    2003-01-01

    Full Text Available The analysis of canonical correlation measures the existence and the intensity of the association between two groups of variables. The research objectified to evaluate thecanonical correlation between chemical and physical characteristics and fiber dimensional ofwood of Eucalyptus grandis and Eucalyptus saligna clones, verifying the interdependenceamong the groups of studied variables. The analysis indicated that the canonical correlationswere high and that in two cases the first and second pair were significant at the level of 1% ofprobability. The analysis of canonical correlation showed that the groups are notindependent. The intergroup associations indicated that the wood of high insoluble lignin contentand low ash content is associated with the high radial and tangential contraction and highbasic density wood.

  11. Biomass of Eucalyptus grandis coffs harbour, by growth-ring identification using the gamma-ray attenuation technique

    International Nuclear Information System (INIS)

    Rezende, M.A.

    1984-01-01

    Samples of 13-year old Eucalyptus grandis were analysed using the gamma-ray attenuation technique. The density was obtained through the profile, and it was then possible to determine the mean density for each annual ring of the samples. The period from formation of two consecutive late-wood rings, i.e., two high density peaks, was considered as one year of tree life. An attempt was made to determine the annual increments at breast-heigh diameter, per height unit, based on volume, mass and energy, trying to estimate the cutting age for each of these elements and the relationship between them. Emphasis was also put on the importance with which density variations, and core to cork calorific value, should be considered when determining the cutting age. (M.A.C.) [pt

  12. Características tecnológicas das madeiras de eucalyptus grandis w.hill ex maiden e eucalyptus cloeziana f. muell visando ao seu aproveitamento na indústria moveleira

    OpenAIRE

    Joaquim Carlos Gonçalez; Lílian de Cássia S. Breda; João Francisco M. Barros; Denízia Gonçalves Macedo; Gerard Janin; Alexandre Florian Da Costa; Ailton Teixeira do Vale

    2006-01-01

    This work was carried out at Universidade de Brasília at the Wood Products of Forestry Laboratory (IBAMA, Brasília, DF, Brazil). Two species of eucalypt wood (Eucalyptus grandis W.Hill ex Maiden and Eucalyptus cloeziana) were studied to supply the furniture making industry. The wood of Eucalyptus grandis W.Hill ex Maiden shows physical properties (density and retractibility) and mechanical properties (static bending and hardness) very suitable for the industry of wood furniture. S...

  13. MANEJO DE RESIDUOS DE Eucalyptus grandis Hill ex Maiden EN SITIOS CONTRASTANTES

    Directory of Open Access Journals (Sweden)

    Ana Maria Lupi

    2017-01-01

    Full Text Available En la región NE de Argentina existe poca información sobre el impacto que tienen las diferentes alternativas de manejo de residuos de la cosecha forestal sobre las propiedades edáficas. En este estudio se analizó el efecto a corto plazo de sistemas de manejo de residuos de la cosecha de Eucalyptus grandis Hill ex Maiden, sobre la concentración del carbono orgánico total del suelo (COT y la fracción particulada (COP, su estado estructural y la conductividad hidráulica saturada. El estudio se desarrolló en dos sitios de la Provincia de Entre Ríos, Argentina, en un suelo Aquic Hapluderts (S1 y en un Fluventic Hapludolls (S2. Se aplicaron los siguientes tratamientos: 1. Extracción total de residuos (ER, 2. Conservación de residuos (CR, 3. Quema de residuos-testigo (QR, 4. Doble adición de residuos (DAR. El muestreo de suelos en las profundidades 0-5 cm y 5-10 cm se realizó a los 18 (S1 y a los 22 (S2 meses de aplicar los tratamientos. En el corto plazo, el efecto de los tratamientos dependió del tipo de suelo y de la variable analizada. Los contrastes más importantes se debieron al efecto de las características intrínsecas del suelo sobre el tamaño de los agregados y la conductividad hidráulica saturada. La conservación de residuos no aumentó la concentración de COT ni modificó la calidad de la materia orgánica medida a partir de COP. El suelo Aquic Hapluderts presentó agregados más grandes, más estables y una mayor K sat en comparación con el Fluventic Hapludolls. En el Aquic Hapluderts se observó una mayor conductividad hidráulica saturada en los tratamientos con conservación de los residuos (DAR y CR, debido a que la cobertura preservaría el sistema poroso en la interfase suelo-atmosfera. El Fluventic Hapludolls fue menos estable y los tratamientos no modificaron significativamente el tamaño de los agregados ni la conductividad hidráulica saturada . Considerando que las características del sitio tienen efectos

  14. Potensi Kayu Perkakas dan Kayu Bakar Jenis Jati (Tectona grandis di Hutan Rakyat Desa Natah, Gunung Kidul

    Directory of Open Access Journals (Sweden)

    Ris Hadi Purwanto

    2009-07-01

    Full Text Available Potentials  of Merchantable Timber and Firewod of Teak (Tectona grandis in Natah Village Community Forest , Gunungkidul The potential of merchantable timber and firewod of teak (Tectona grandis in Desa Natah community forest was estimated by developing allometric equations method. To establish the allometric equation 350 sample trees were measured to determine the relationships between tree height (H and diameter breast height (D. Thirty trees of various sizes were cut to measure the merchantable timber and firewood volume. The raw merchantable timber volume of teak in the community forest was defined as the ligneous material contained in the bole and branches which both with a diameter of at least 10cm. The result showed that D (taken at about 1.3m above the ground was a good predictor of H with r2 over 0.9672. When D was combined with H, r2 was improved somewhat for the merchantable timber volume, suggesting the growth patterns of tree dimensions were colesy interdependent. A standing stock of the merchantable timber and firewood volume of teak in the community forest was then estimated based on the allometric relations. Proportions of the merchantable timber and firewood volume were 66.91% and 33.09% of total wood volume per tree, respectively. The potential of merchantable timber and firewood volume in these community forest were 13.501 m3/ha and 8.686 m3/ha, respectively, with a basal area of 1.887 m2/ha. Based on the basal area, Desa Natah community forests of teak could be classified into extremely sparse of stands category.

  15. EFEITO DA TERMORRETIFICAÇÃO NAS PROPRIEDADES MECÂNICAS DAS MADEIRAS DE Pinus taeda E Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Karina Soares Modes

    2017-01-01

    Full Text Available This study investigated the effect of thermal treatment, employed under two treatment conditions on mechanical properties of wood of Pinus taeda L. and Eucalyptus grandis W. Hill ex Maiden. From each species, three 25-year tress were sampled, and from each one, it was taken the first 2-meter long log, which was obtained from two boards of size 7,0 x 20,0 cm (thickness x width respectively diametrically opposite from where 30 specimens were saw for each treatment. At the first one, called the combined one, the woods were subjected to heat treatment by autoclaving at 130 / ± 3°C and pressure of 2 kgf / cm ² for 3 hours and, after a conditioning period, subjected to heat in an electric oven at 160 / ± 1°C for the same period. The second treatment consisted only of heat treatment in oven. It was also evaluated the pieces of wood without treatment (control. The mechanical properties were evaluated by means of tests for determining the modulus of elasticity and rupture in bending, maximum resistance to compression parallel to the grain and Janka hardness according to ASTM D 143 (1995, and the impact resistance according to ABNT NBR 7190 (1997. For Pinus taeda wood, it was observed that treatment in an oven gave the worst outcomes, both due to the reduction in the values of supported load of a greater number of mechanical properties evaluated, but also as compared to the lowest increments in resistance when it was observed an increase to the same ones with heat treatment. In Eucalyptus grandis, the combined treatment decreased the greatest number of mechanical properties of wood.

  16. INFLUÊNCIA DO VERMICOMPOSTO NA PRODUÇÃO DE MUDAS DE Eucalyptus grandis Hill ex Maiden

    Directory of Open Access Journals (Sweden)

    Mauro Valdir Schumacher

    2001-03-01

    Full Text Available Considerando a expressiva área de florestas plantadas com espécies do gênero Eucalyptus e o uso dessa madeira para múltiplos propósitos, sua cultura é de grande importância econômica para o Brasil. O uso de substratos com boas características físico-químicas constituem importante fator para a qualidade das mudas produzidas. Neste sentido, o objetivo do trabalho foi avaliar o desenvolvimento de diferentes doses de vermicomposto em tubetes com capacidade de receber aproximadamente 50 cm3 de substrato. O estudo foi conduzido em casa de vegetação com Eucalyptus grandis, no período de janeiro a abril de 1997. O substrato utilizado para produção das mudas foi o solo coletado no horizonte A (0-20 cm da Unidade de Mapeamento São Pedro (Argissolo Vermelho-Amarelo acrescido de diferentes doses de vermicomposto (0; 5,0; 10,0; 15,0 e 20,0 cm3 por tubete. O vermicomposto utilizado foi proveniente de produção de minhocas vermelhas da Califórnia (Eisenia foetida Savigny valendo-se de esterco de bovino. Após 100 dias de desenvolvimento, observou-se que as mudas de Eucalyptus grandis responderam de maneira distinta às doses aplicadas, não suportando misturas acima 15,0 cm3 (30% de vermicomposto por tubete.

  17. Influência do vermicomposto na produção de mudas de Eucalyptus grandis Hill ex Maiden.

    Directory of Open Access Journals (Sweden)

    Mauro Valdir Schumacher

    2010-08-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 Considerando a expressiva área de florestas plantadas com espécies do gênero Eucalyptus e o uso dessa madeira para múltiplos propósitos, sua cultura é de grande importância econômica para o Brasil. O uso de substratos com boas características físico-químicas constituem importante fator para a qualidade das mudas produzidas. Neste sentido, o objetivo do trabalho foi avaliar o desenvolvimento de diferentes doses de vermicomposto em tubetes com capacidade de receber aproximadamente 50 cm3 de substrato. O estudo foi conduzido em casa de vegetação com Eucalyptus grandis, no período de janeiro a abril de 1997. O substrato utilizado para produção das mudas foi o solo coletado no horizonte A (0-20 cm da Unidade de Mapeamento São Pedro (Argissolo Vermelho-Amarelo acrescido de diferentes doses de vermicomposto (0; 5,0; 10,0; 15,0 e 20,0 cm3 por tubete. O vermicomposto utilizado foi proveniente de produção de minhocas vermelhas da Califórnia (Eisenia foetida Savigny valendo-se de esterco de bovino. Após 100 dias de desenvolvimento, observou-se que as mudas de Eucalyptus grandis responderam de maneira distinta às doses aplicadas, não suportando misturas acima 15,0 cm3 (30% de vermicomposto por tubete.

  18. cDNA sequencing improves the detection of P53 missense mutations in colorectal cancer

    International Nuclear Information System (INIS)

    Szybka, Malgorzata; Kordek, Radzislaw; Zakrzewska, Magdalena; Rieske, Piotr; Pasz-Walczak, Grazyna; Kulczycka-Wojdala, Dominika; Zawlik, Izabela; Stawski, Robert; Jesionek-Kupnicka, Dorota; Liberski, Pawel P

    2009-01-01

    Recently published data showed discrepancies beteween P53 cDNA and DNA sequencing in glioblastomas. We hypothesised that similar discrepancies may be observed in other human cancers. To this end, we analyzed 23 colorectal cancers for P53 mutations and gene expression using both DNA and cDNA sequencing, real-time PCR and immunohistochemistry. We found P53 gene mutations in 16 cases (15 missense and 1 nonsense). Two of the 15 cases with missense mutations showed alterations based only on cDNA, and not DNA sequencing. Moreover, in 6 of the 15 cases with a cDNA mutation those mutations were difficult to detect in the DNA sequencing, so the results of DNA analysis alone could be misinterpreted if the cDNA sequencing results had not also been available. In all those 15 cases, we observed a higher ratio of the mutated to the wild type template by cDNA analysis, but not by the DNA analysis. Interestingly, a similar overexpression of P53 mRNA was present in samples with and without P53 mutations. In terms of colorectal cancer, those discrepancies might be explained under three conditions: 1, overexpression of mutated P53 mRNA in cancer cells as compared with normal cells; 2, a higher content of cells without P53 mutation (normal cells and cells showing K-RAS and/or APC but not P53 mutation) in samples presenting P53 mutation; 3, heterozygous or hemizygous mutations of P53 gene. Additionally, for heterozygous mutations unknown mechanism(s) causing selective overproduction of mutated allele should also be considered. Our data offer new clues for studying discrepancy in P53 cDNA and DNA sequencing analysis

  19. [Construction of phage display cDNA library from adult worms of Schistosoma japonicum].

    Science.gov (United States)

    Sun, Yi; Jia, Ren-chu; Liu, Jin-ming; Yuan, Chun-xiu; Shi, Yao-jun; Lu, Ke; Fu, Zhi-qiang; Sun, Huan; Cai, You-min; Lin, Jiao-jiao

    2007-10-01

    To screen protective antigen genes and construct the T7 phage display library from adult worms of Schistosoma japonicum. Total RNA was extracted from adult worms of S. japonicum by Trizol reagent anti mRNA was isolated from the total RNA. The ds cDNA was synthesized by reverse transcription using random primer. Directional EcoR I/ Hind III linkers were ligated into the ends of ds cDNA and the ds cDNA was digested with EcoR I anti Hind III, which resulted in ds cDNA with EcoR I and Hind III adhering ends. The digested ds cDNA fragments longer than 300 bp in length were fractionated and ligated into T7 Select 10-3b vector. After packaging in citro, the T7 Select 10-3b vector was transformed into BLT5403 to construct the T7 phage display cDNA library. Plaque assay and PCR were used to evaluate the library. Seven known objective genes of S. japonicum were screened by PCR to detect the representation of the library. Primary library capacity was 4.98 x 10(6) pfu, and the titer of amplified library was 3.85 x 10(11) pfu/mL. The PCR identification result of 96 clones picked at random showed that recombination rate was 93.8%, in which 95.6% inserted cDNA fragments were longer than 300 bp in length. All the seven known objective genes of S. japonicum were amplified from the library. The T7 phage display library from adult worms of Schistosoma japonicum was constructed.

  20. [Construction of cDNA expression library of unfed female Haemaphysalis longicornis and immuno-screening].

    Science.gov (United States)

    Chai, Hui-ping; Liu, Guang-yuan; Zhang, Lin; Gong, Zhen-li; Xie, Jun-ren; Tian, Zhan-cheng; Wang, Lu; Jia, Ning

    2009-02-28

    To construct a cDNA expression library from unfed female tick Haemaphysalis longicornis for screening and cloning potential antigenic genes. Total RNA was isolated from unfed female ticks, mRNA was purified and a library of oligo (dT) -primed cDNA with added directional EcoR I /Hind III linkers was constructed from the purified mRNA. The constructed cDNA was ligated to the EcoR I /Hind III arms of the lambda SCREEN vector. Pure phage stocks were harvested by plaque purification and converted to plasmid subclones by plating phage on host strain BM25.8. Recombinant plasmids that were subcloned to E. coli BM25.8 were isolated and transformed into E. coli JM109. Recombinant plasmids abstracted from JM109 were identified by PCR and sequencing. The recombinant phage DNA was packaged by using phage-marker packaging extracts, resulting in a primary cDNA library with a size of 1.8 x 10(6) pfu. Data showed 100% of the library were recombinant and the titer of the amplified library was 2.4 x 10(9) pfu/ml. Forty-two clones of encoding immunodominant antigens were obtained from the cDNA library. Sequence analysis revealed 12 unique cDNA sequences and the encoded putative proteins showed similarities to H. longicornis tropomyosin mRNA, Rhipicephalus annulatus unknown larval protein mRNA, chromosome 2R of Drosophila melanogaster, mitochondrial DNA of H. flava, clones HqL09 unkown mRNA and Hq05 mRNA of H. qinghaiensis, and myosin alkali light chain protein mRNA. The cDNA expression library from unfed female H. longicornis was successfully constructed and screening of protective genes may provide candidate antigens of the tick.

  1. Isolation and characterization of the murine alpha-L-iduronidase cDNA

    Energy Technology Data Exchange (ETDEWEB)

    Clarke, L.A.; Zhang, H. Nasir, J. [Univ. of British Columbia, Vancouver (Canada)] [and others

    1994-09-01

    Mucopolysaccharidosis I (MPS I) are a group of disorders caused by deficiency of the lysosomal enzyme alpha-L-iduronidase. The characterization of the human gene and the identification of mutations underlying MPS I in humans has led to the delineation of the molecular basis of this disorder. Model systems are now needed for the evaluation and development of therapeutics for this disorder. Both canine and feline models for MPS type I have been described but only the canine gene has been isolated and characterized. We report here the cloning and expression of the murine alpha-L-iduronidase cDNA. The murine cDNA was obtained by screening a mouse liver cDNA library with a probe from the human cDNA. The full length murine cDNA is 3120 base pairs in length and thus is considerably larger than both the human and canine transcripts. The increase in size is due to a 1.2 kb 3{prime} untranslated region in the murine cDNA that contains a CA dinucleotide repeat. Within the coding region the murine cDNA shows sequences. At the protein level the murine protein shows 77% similarity with the human protein and 75% similarity with the canine protein. There are significant differences in both the start and stop sites with the murine protein 9 amino acids shorter at both the N terminal signal peptide region and the C terminus. Expression of the murine cDNA in COS-1 cells resulted in a 20 fold increase in intracellular alpha-L-iduronidase activity as well as the detection of considerable enzyme activity in the culture medium. Comparison of the reported missense mutations underlying MPS I in humans (A75T, H82P, R89Q, L218P, P533R, Q310X, T366P) has shown conservation of these amino acid residues in the murine protein. The isolation of the murine iduronidase cDNA will now allow for the development of a murine model for MPS I.

  2. Digital analysis of cDNA abundance; expression profiling by means of restriction fragment fingerprinting

    Directory of Open Access Journals (Sweden)

    Regenbogen Johannes

    2002-03-01

    Full Text Available Abstract Background Gene expression profiling among different tissues is of paramount interest in various areas of biomedical research. We have developed a novel method (DADA, Digital Analysis of cDNA Abundance, that calculates the relative abundance of genes in cDNA libraries. Results DADA is based upon multiple restriction fragment length analysis of pools of clones from cDNA libraries and the identification of gene-specific restriction fingerprints in the resulting complex fragment mixtures. A specific cDNA cloning vector had to be constructed that governed missing or incomplete cDNA inserts which would generate misleading fingerprints in standard cloning vectors. Double stranded cDNA was synthesized using an anchored oligo dT primer, uni-directionally inserted into the DADA vector and cDNA libraries were constructed in E. coli. The cDNA fingerprints were generated in a PCR-free procedure that allows for parallel plasmid preparation, labeling, restriction digest and fragment separation of pools of 96 colonies each. This multiplexing significantly enhanced the throughput in comparison to sequence-based methods (e.g. EST approach. The data of the fragment mixtures were integrated into a relational database system and queried with fingerprints experimentally produced by analyzing single colonies. Due to limited predictability of the position of DNA fragments on the polyacrylamid gels of a given size, fingerprints derived solely from cDNA sequences were not accurate enough to be used for the analysis. We applied DADA to the analysis of gene expression profiles in a model for impaired wound healing (treatment of mice with dexamethasone. Conclusions The method proved to be capable of identifying pharmacologically relevant target genes that had not been identified by other standard methods routinely used to find differentially expressed genes. Due to the above mentioned limited predictability of the fingerprints, the method was yet tested only with

  3. Generation of cDNA expression libraries enriched for in-frame sequences.

    Science.gov (United States)

    Davis, C A; Benzer, S

    1997-03-18

    Bacterial cDNA expression libraries are made to reproduce protein sequences present in the mRNA source tissue. However, there is no control over which frame of the cDNA is translated, because translation of the cDNA must be initiated on vector sequence. In a library of nondirectionally cloned cDNAs, only some 8% of the protein sequences produced are expected to be correct. Directional cloning can increase this by a factor of two, but it does not solve the frame problem. We have therefore developed and tested a library construction methodology using a novel vector, pKE-1, with which translation in the correct reading frame confers kanamycin resistance on the host. Following kanamycin selection, the cDNA libraries contained 60-80% open, in-frame clones. These, compared with unselected libraries, showed a 10-fold increase in the number of matches between the cDNA-encoded proteins made by the bacteria and database protein sequences. cDNA sequencing programs will benefit from the enrichment for correct coding sequences, and screening methods requiring protein expression will benefit from the enrichment for authentic translation products.

  4. [Construction of a yeast two-hybrid cDNA library from the human testis].

    Science.gov (United States)

    Zheng, Ying; Zhang, Lu-Ping; Jia, Xiao-Qin; Wang, Hai-Yan

    2012-04-01

    To construct a human testis cDNA library for yeast two-hybrid screening. Human normal testis mRNA was purified from total RNA, and ds cDNA was synthesized and amplified using primers SMART III and CDS III oligo (dT) as the base of recombination. The purified PCR products and linearized plasmid pGADT7-Rec were co-transformed into the competent yeast Y187 and recombined by yeast homologous recombinase in the yeast cells to form an active cyclic plasmid. All the clones growing on the SD/-Leu plates were harvested to constitute a human testis cDNA library. We constructed a human testis cDNA library with high multiplication and adequate capacity, from which 2.0 x 10(6) recombinants were obtained. The amplified PCR fragments were between 0.3 kb and 4.0 kb in length. The yeast two-hybrid cDNA library of human testis was successfully constructed by the Clontech SMART method, which has prepared a ground for further studies on the molecular mechanism of spermatogenesis.

  5. [Construction of cDNA expression library of salivary gland from Boophilus microplus].

    Science.gov (United States)

    Tian, Zhan-Cheng; Liu, Guang-Yuan; Xie, Jun-Ren; Gong, Zhen-Li

    2008-10-30

    Total RNA were isolated from salivary gland dissected from partially engorged Boophilus microplus. The mRNA was purified. A library of oligo (dT)-primed cDNA with added directional EcoR I/Hind III linkers was constructed from the purified mRNA. The constructed cDNA was ligated to the EcoR I/Hind III arms of the lambda SCREEN vector. The recombinant phage DNA was packaged by phage-marker packaging extracts, resulting in a primary cDNA library with a size of 1.38x10(6) PFU. Data showed 100% of the library were recombinant and the titer of the amplified library was 2x10(9) PFU/ml. A partial cDNA encoding cytochrome oxidase C subunit II of B. microplus was screened from the expression library with rabbit serum against B. microplus salivary gland proteins. The results is suggested that the cDNA expression library has been constructed.

  6. Construction of equalized short hairpin RNA library from human brain cDNA.

    Science.gov (United States)

    Xu, Lei; Li, Jingqi; Liu, Li; Lu, Lixia; Gao, Jingxia; Li, Xueli

    2007-02-20

    Short hairpin RNA (shRNA) library is a powerful new tool for high-throughput loss-of-function genetic screens in mammalian cells. An shRNA library can be constructed from synthetic oligonucleotides or enzymatically cleaved natural cDNA. Here, we describe a new method for constructing equalized shRNA libraries from cDNA. First, enzymatically digested cDNA fragments are equalized by a suppression PCR-based method modified from suppression subtractive hybridization. The efficiency of equalization was confirmed by quantitative real-time PCR. The fragments are then converted into an shRNA library by a series of enzymatic treatments. With this new technology, we constructed a library from human brain cDNA. Sequence analysis showed that most of the randomly selected clones had inverted repeat sequences converted from different cDNA. After transfecting HEK 293T cells and detecting gene expression, three out of eight clones were demonstrated to significantly inhibit their target genes.

  7. Molecular cloning of goat 20alpha-hydroxysteroid dehydrogenase cDNA.

    Science.gov (United States)

    Jayasekara, Walimuni Samantha Nilanthi; Yonezawa, Tomohiro; Ishida, Maho; Yamanouchi, Keitaro; Nishihara, Masugi

    2004-06-01

    20Alpha-hydroxysteroid dehydrogenase (20alpha-HSD), which catalyzes the conversion of progesterone to its inactive form 20alpha-dihydroprogesterone, is expressed in murine placenta and has been suggested to play roles in maintaining pregnancy. To understand the role of 20alpha-HSD during pregnancy in the goat, as a first step, cloning and sequencing of 20alpha-HSD cDNA were performed. The full nucleotide sequence of 20alpha-HSD cDNA was determined on samples obtained from the corpus luteum at the luteal phase of the estrous cycle and the placenta in late pregnancy by RT-PCR and 3' and 5' RACE systems. Cloned 20alpha-HSD cDNA consisted of 1124 bp and belonged to the aldo-keto reductase superfamily. From the start codon to stop codon there were 323 amino acids, the same as in other species. To verify whether the protein derived from goat 20alpha-HSD cDNA had 20alpha-HSD activity, the cDNA was expressed by bacteria. Bacterially expressed goat 20alpha-HSD protein showed 20alpha-HSD enzyme activity. A tissue distribution study demonstrated that 20alpha-HSD was expressed in the placenta, but not in the adrenal gland, liver and spleen during pregnancy. The present study suggests that goat 20alpha-HSD is another member of the aldo-keto reductase superfamily and that it plays a role in the placenta during pregnancy.

  8. [Combining SSH and cDNA microarray for identification of lung cancer related genes].

    Science.gov (United States)

    Fan, Baoxing; Zhang, Kaitai; Da, Jiping; Xie, Ling; Wang, Shengqi; Wu, Dechang

    2003-04-20

    To screen and identify differentially expressed genes among lung cancer tissues, paracancerous pulmonary tissues and some other kinds of tumor tissues using suppression subtractive hybridization (SSH) and cDNA Microarray. One cDNA chip was made by gathering clones of three differentially expressed cDNA libraries which came from BEP2D cell lines during three different malignant transformed phases. Then the clones were hybridizated with cDNA probes which extracted from 15 cases of lung cancer tissues, 5 cases of paracancerous pulmonary tissues and 24 cases of other 8 kinds of tumor tissues respectively. Twenty-six cDNAs were obtained which expressed higher in lung cancer tissues than that in paracancerous pulmonary tissues. Thirty-one cDNAs expressed remarkably higher in paracancerous tissues than those in cancer tissues. Compared with other 8 kinds of tumors, paracancerous tissues had 63 overexpressed cDNAs and lung cancer tissues had 87 overexpressed cDNAs. The combination of SSH and cDNA microarray is rapid and effective for screening and identification of differentially expressed genes in different samples. It may be potentially useful for diagnosis of lung cancer to further study the differentially expressed genes among lung cancer tissues, paracancerous pulmonary tissues and other tumor tissues.

  9. Sequence of a cDNA encoding turtle high mobility group 1 protein.

    Science.gov (United States)

    Zheng, Jifang; Hu, Bi; Wu, Duansheng

    2005-07-01

    In order to understand sequence information about turtle HMG1 gene, a cDNA encoding HMG1 protein of the Chinese soft-shell turtle (Pelodiscus sinensis) was amplified by RT-PCR from kidney total RNA, and was cloned, sequenced and analyzed. The results revealed that the open reading frame (ORF) of turtle HMG1 cDNA is 606 bp long. The ORF codifies 202 amino acid residues, from which two DNA-binding domains and one polyacidic region are derived. The DNA-binding domains share higher amino acid identity with homologues sequences of chicken (96.5%) and mammalian (74%) than homologues sequence of rainbow trout (67%). The polyacidic region shows 84.6% amino acid homology with the equivalent region of chicken HMG1 cDNA. Turtle HMG1 protein contains 3 Cys residues located at completely conserved positions. Conservation in sequence and structure suggests that the functions of turtle HMG1 cDNA may be highly conserved during evolution. To our knowledge, this is the first report of HMG1 cDNA sequence in any reptilian.

  10. Genetic and environmental characterization of Abies alba Mill. populations at its western rear edge

    Directory of Open Access Journals (Sweden)

    Sancho-Knapik, D.

    2014-12-01

    Full Text Available The genetic structure of ten Abies alba populations at the western rear edge in the Spanish Pyrenees was characterized and compared with two German populations, looking for the role of climatic factors in the fir decline. Growth, defoliation, aridity and cumulative summer water deficit summer were also characterized. Spanish populations show a lower genetic diversity and a high genetic differentiation than German ones, mainly established by an East (higher diversity-West (lower diversity gradient. The three defoliated populations are the western ones, with higher summer aridity. Contrastingly, the southern population (Guara shows climatic variables close to western sites but without defoliation and with a higher genetic diversity, indicating a possible adaptation to the sub-Mediterranean conditions. Silver fir in the Spanish Pyrenees constitutes a “stable” rear edge because of their isolation, small sized and small genetic diversity. Western Pyrenean sites subjected to dryer conditions and presenting lower genetic diversity are prone to drought-induced mortality in the context of global warming.Se ha caracterizado la estructura genética de diez poblaciones de Abies alba en la retaguardia occidental de su distribución en el Pirineo español en comparación con dos poblaciones de Alemania, buscando la influencia de factores climáticos en el decaimiento del abeto. También se caracterizó crecimiento, defoliación, aridez y déficit acumulado de agua durante el verano. Las poblaciones españolas mostraron una menor diversidad genética y una mayor diferenciación genética que las poblaciones alemanas, establecida principalmente a través de un eje Este (mayor diversidad-Oeste (menor diversidad. Las tres poblaciones con defoliación son las del suroeste, con mayor aridez estival. Por el contrario, la población más meridional (Guara muestra variables climáticas cercanas a estas poblaciones del suroeste pero sin defoliación y con mayor

  11. Expressed sequence tags: normalization and subtraction of cDNA libraries expressed sequence tags\\ normalization and subtraction of cDNA libraries.

    Science.gov (United States)

    Soares, Marcelo Bento; de Fatima Bonaldo, Maria; Hackett, Jeremiah D; Bhattacharya, Debashish

    2009-01-01

    Expressed Sequence Tags (ESTs) provide a rapid and efficient approach for gene discovery and analysis of gene expression in eukaryotes. ESTs have also become particularly important with recent expanded efforts in complete genome sequencing of understudied, nonmodel eukaryotes such as protists and algae. For these projects, ESTs provide an invaluable source of data for gene identification and prediction of exon-intron boundaries. The generation of EST data, although straightforward in concept, requires nonetheless great care to ensure the highest efficiency and return for the investment in time and funds. To this end, key steps in the process include generation of a normalized cDNA library to facilitate a high gene discovery rate followed by serial subtraction of normalized libraries to maintain the discovery rate. Here we describe in detail, protocols for normalization and subtraction of cDNA libraries followed by an example using the toxic dinoflagellate Alexandrium tamarense.

  12. Flux de CO2 dans les lagunes (Aby et Ebrié) de la Côte d'Ivoire.

    OpenAIRE

    Koné, Yéfanlan José-Mathieu; Seu-Anoi, N.M.

    2010-01-01

    Les données de CO2 obtenues pendant deux campagnes dans les lagunes Aby et Ebrié montrent que ces écosystèmes sont très hétérogènes en terme de dynamique de CO2. Alors que la lagune Ebrié est sursaturée de CO2 vis-à-vis de l'atmosphère , la lagune Aby quant à elle est sous saturée en CO2 à cause d'une forte production phytoplanctonique caractérisée par des valeurs élevées de chlorophylle a dans la colonne d'eau. Ces résultats ne permettent pas par conséquent de conclure si les lagunes sont de...

  13. Ecological and morphological characteristics of pyramidal fir (Abies alba var. pyramidalis in the locality of Ogorijevac (the Pester plateau

    Directory of Open Access Journals (Sweden)

    Ratknić M.

    2013-01-01

    Full Text Available The paper presents environmental conditions and morphological characteristics of pyramidal fir (Abies alba var. pyramidalis in the locality of Ogorijevac in southwestern Serbia. Pyramidal fir trees grow in three localities in Europe, but only as individual trees. A site of pyramidal fir (Abies alba var. pyramidalis in the locality of Ogorijevac near the town of Sjenica in Serbia was first studied by Tošić in 1963, who described some characteristics of the locality in which the pyramidal fir occurs and some morphological characteristics of the trees. This paper provides a detailed description of the parent rock, soil, climate characteristics of the locality and morphological characteristics of the trees. [Projekat Ministarstva nauke Republike Srbije, br. 43007: Studying climate change and its influence on the environment: Impacts, adaptation and mitigation

  14. Optimal management and productivity of Eucalyptus grandis on former phosphate mined and citrus lands in central and southern Florida: influence of genetics and spacing

    Science.gov (United States)

    Kyle W. Fabbro; Donald L. Rockwood

    2016-01-01

    Eucalyptus short rotation woody crops (SRWC) with superior genotypes are promising in central and south Florida due to their fast growth, freeze resilience, coppicing ability, and site tolerance. Four Eucalyptus grandis cultivars, E.nergy™ G1, G2, G3, and/or G5, were established in 2009 at varying planting densities on a...

  15. Integrating remote sensing and ancillary data for regional ecosystem assessment: eucalyptus grandis agro-system in kwazulu-Natal, South Africa

    CSIR Research Space (South Africa)

    Cho, Moses A

    2009-07-01

    Full Text Available . Furthermore, they cannot usually be extended over regional scales of vegetation because of logistics and huge number of sample variations[3]. Second, it has long been established that environmental variables including climatic, edaphic and topographic..., site quality and topographic variables for estimating ecosystem state, - parameterise radiative transfer models (leaf and canopy reflectance models) for estimating E. grandis ecosystem state variables - and lastly, develop integrated models...

  16. Standardization of a protocol to obtain genomic DNA for the quantification of 5mC in epicormics buds of Tectona grandis L.

    OpenAIRE

    Elisa Quiala; Luis Valledor; Rodrigo Hazbun; Raúl Barbón; Manuel de Feria; Maité Chávez

    2008-01-01

    The present investigation was carried out with the objective of defining an extraction and purification method that it provided deoxyribonucleic acid (DNA) appropriate to determine the percentage of 5mC in the genomic DNA of epicormics buds of Tectona grandis L. During the standardization of the protocol four methods were compared: 1 -classic based on saline shock solution with CTAB (hexadecil trimetil ammonium bromide), 2 - Kit of extraction of DNA plants DNeasy Plant Mini Kit (QIAGEN) accor...

  17. ESTs, cDNA microarrays, and gene expression profiling: tools for dissecting plant physiology and development.

    Science.gov (United States)

    Alba, Rob; Fei, Zhangjun; Payton, Paxton; Liu, Yang; Moore, Shanna L; Debbie, Paul; Cohn, Jonathan; D'Ascenzo, Mark; Gordon, Jeffrey S; Rose, Jocelyn K C; Martin, Gregory; Tanksley, Steven D; Bouzayen, Mondher; Jahn, Molly M; Giovannoni, Jim

    2004-09-01

    Gene expression profiling holds tremendous promise for dissecting the regulatory mechanisms and transcriptional networks that underlie biological processes. Here we provide details of approaches used by others and ourselves for gene expression profiling in plants with emphasis on cDNA microarrays and discussion of both experimental design and downstream analysis. We focus on methods and techniques emphasizing fabrication of cDNA microarrays, fluorescent labeling, cDNA hybridization, experimental design, and data processing. We include specific examples that demonstrate how this technology can be used to further our understanding of plant physiology and development (specifically fruit development and ripening) and for comparative genomics by comparing transcriptome activity in tomato and pepper fruit.

  18. Construction and analysis of full-length and normalized cDNA libraries from citrus.

    Science.gov (United States)

    Marques, M Carmen; Perez-Amador, Miguel A

    2012-01-01

    We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of genes.

  19. Design and Screening of M13 Phage Display cDNA Libraries

    Directory of Open Access Journals (Sweden)

    Yuliya Georgieva

    2011-02-01

    Full Text Available The last decade has seen a steady increase in screening of cDNA expression product libraries displayed on the surface of filamentous bacteriophage. At the same time, the range of applications extended from the identification of novel allergens over disease markers to protein-protein interaction studies. However, the generation and selection of cDNA phage display libraries is subjected to intrinsic biological limitations due to their complex nature and heterogeneity, as well as technical difficulties regarding protein presentation on the phage surface. Here, we review the latest developments in this field, discuss a number of strategies and improvements anticipated to overcome these challenges making cDNA and open reading frame (ORF libraries more readily accessible for phage display. Furthermore, future trends combining phage display with next generation sequencing (NGS will be presented.

  20. [Screening of specifically expressed genes in amphioxus neurula by construction of a subtractive cDNA library].

    Science.gov (United States)

    Zhang, Lei; Yang, Yong-Jie; Zhang, Yan-Jun

    2010-12-01

    To screen specifically expressed genes in the development of nerve, muscle, and body axis of amphioxus, Branchiostoma belcheri tsingtauenese. A subtractive cDNA library was constructed from the 12-hour amphioxus neurula cDNA after subtractively hybridized with the 6-hour amphioxus gastrula cDNA. The total RNA was extracted from the 12-hour neurula and 6-hour gastrula, then reverse transcribed into cDNA. The 12-hour neurula cDNA was designated as the experimental group (the tester) and the 6-hour gastrula cDNA as the control group (the driver). The differentially expressed sequences were exponentially amplified using suppression PCR. Background was subtracted and differentially expressed sequences were further enriched. The PCR products were ligated to the T Vector. After transformation of the recombinant plasmid carrying inserted amphioxus cDNA into E.coli host cells, the cDNA library was constructed successfully. Two hundred randomly chosen positive clones were sequenced and some of neurula-specifically expressed genes were obtained. SSH is an effective method for searching differentially expressed genes. The subtractive cDNA library we generated provides a tool for further study of regulatory mechanisms of amphioxus early embryonic development.