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Sample records for abacates persia americana

  1. Experimental study of convective coefficient of mass transfer of avocado (Persia americana Mill.)

    Alves, Suerda Bezerra; Luiz, Marcia Ramos; Amorim, Joselma Araujo de; Gusmao, Rennam Pereira de; Gurgel, Jose Mauricio [Universidade Federal da Paraiba (LES/UFPB), Joao Pessoa, PB (Brazil). Lab. de Energia Solar

    2010-07-01

    Most of all energy consumed worldwide comes from fossil fuels derived from petroleum. With the petroleum crisis in the 70 were sought new energy sources, among them renewable. One such source is biodiesel energy, organic matter originated from animal and/or vegetable. Among the various plant species is the avocado (Persia americana Mill.) showing great potential in the production of petroleum extracted from the pulp and the alcohol removed from the seed. The main obstacle for obtaining the petroleum is the high humidity found in the pulp, being necessary to the drying process, which involves the transfer of heat and mass. The aim of this study was to use the mathematical model represented by Newton's Law of Cooling to simulate the mass transfer on the surface of the avocado pulp during the drying process. The equation of the mathematical model was solved numerically and the method of least squares was identified convective coefficient of Mass Transfer. The dryer used in the experimental process was operated with air flow in the vertical, air flow average fixed 3m/s and temperatures of 50, 60 and 70 deg C. The scheme of the dryer used in the research is composed of the following equipment: centrifugal fan, which drives the air-drying; valve, which allows control of airflow; electrical resistance, used for heating air; the drying chamber, where enables measurement of temperature and relative humidity; support for smaller trays; trays smaller, where the samples of the pulp of the avocado are placed; exit of the air of drying for the environment. The result presented shows the ratio of moisture content as a function of temperature over time, where it is possible to also observe that how much bigger the temperature of drying, greater will be the convective coefficient of mass transfer of the avocado. (author)

  2. Preliminary in vitro antisickilng properties of crude juice extracts of Persia Americana, Citrus sinensis, Carica papaya and Ciklavit®.

    Iweala, E E J; Uhegbu, F O; Ogu, G N

    2009-12-30

    The antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely Persia americana, Citrus sinensis, and Carica papaya were investigated in vitro alongside a new drug preparation called Ciklavit® that has antisickling activity. Four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoholic extracts were prepared and their antisickling effects on sickle cell trait (HbAS) and sickle cell disease (HbSS) blood samples checked alongside Ciklavit®. Blood samples were stabilized using normal saline and the antisickling effects were checked by counting the number of sickle cells remaining after incubation of the blood samples with the crude fruit extracts and Ciklavit® for twenty-four hours. The results showed that Ciklavit® produced a sustained reduction in the number of sickle cells in both HbAS and HbSS blood samples. Also the alkaline and alcoholic extracts of P. americana and C. papaya produced significant reduction in the number of sickle cells.

  3. Quality of fresh-cut avocado (Persea americana Mill. stored under different temperatures Qualidade de abacates (Persea americana Mill. minimally processados armazenados sob diferentes temperaturas

    Ana Carla Marques Pinheiro

    2009-08-01

    Full Text Available The goal of this work was to evaluate the effect of different storage temperatures on the quality maintenance of fresh-cut 'Fortuna' avocado (Persea americana Mill.. The fruit was selected, washed and sanitized with sodium hypochlorite solution (190 ppm total residual chlorinee for 15 minutes. After that, the tip was cut and the fruit was also cut in halves, peeled and the pit removed, manually. Then the halves were immersed in 0.5% calcium chlorite + 0.5% cysteine solution for 2 minutes, to avoid darkening and packed in rigid plastic covered with 20 micrometer thick plastic wrap (PVC film with a permeability to O2, CO2 and water vapor of 15,000, 80,000 and 390 cm³/m²/day, respectively. The product was stored under 3 different temperatures 0, 5, 10ºC and evaluated at 5 different storege periods of 0, 2, 3, 5 and 6 days, in a randomized design in a 3x5 factorial. The storage of fresh-cut avocado at 5ºC is the best alternative because it determines better maintenance of quality of the avocado. These presented lower darkening of pulp, better scores for appearance and acceptability, lower softening and polyphenoloxidase activity, in comparison to those stored at 0 and 10ºC. The fresh-cut avocados stored at 5ºC presented a shelf life of 5 days, based, mainly on, appearance, score 7.3, sited between the concepts "like moderately" and "like very much" on a hedonic scale of 9 points and 86% of acceptability, at the end of 5 days of storage.Objetivou-se avaliar o efeito de diferentes temperaturas de armazenamento na manutenção da qualidade de abacates 'Fortuna' (Persea americana Mill. minimally processados. Os frutos foram lavados e sanificados em solução de hipoclorito de sódio (190 ppm de cloro residual total por 15 minutos. Posteriormente, efetuou-se a retirada da extremidade onde se insere o pedúnculo, o corte em metades, retirada do caroço e casca, manualmente. Em seguida, as metades foram imersas em solução com cloreto de cálcio 0

  4. Efeito do abacate (Persea americana Mill) variedade hass na lipidemia de ratos hipercolesterolêmicos

    Salgado,Jocelem Mastrodi; BIN, Camila; Mansi,Débora Niero; Souza, Adriana de

    2008-01-01

    Os fitonutrientes podem ser encontrados em muitas frutas, sendo o abacate o mais rico em beta-sitosterol (fitoesterol) e glutationa. O fitoesterol é uma substância de origem vegetal cuja estrutura é muito similar a do colesterol e seu mecanismo de ação envolve a inibição intestinal de absorção do colesterol e diminuição da síntese de colesterol hepático. O efeito é exercido sobre os valores de colesterol plasmático total e LDL, sem afetar os níveis de HDL e triglicérides sanguíneos. O present...

  5. Tolerância a baixas temperaturas de cultivares de abacate (Persea americana Mill.

    Soares Nilberto Bernardo

    2002-01-01

    Full Text Available Em experimento realizado no Núcleo de Agronomia do Sudoeste/IAC, em Capão Bonito (SP, observou-se o comportamento de 13 cultivares de abacate com seis anos de idade: Geada, Pollock e Simmonds (raça antilhana; Antoniolli, Breda, Campinas, Ermor, Jumbo, Margarida, Ouro verde, Reis e Solano (híbridos de antilhana x guatemalense e Fuerte (híbrido de mexicana x guatemalense em relação às baixas temperaturas. As temperaturas do ar registradas no posto meteorológico do local foram: -2,8degreesC; -0,4degreesC; -0,2degreesC e -1,8degreesC, respectivamente, nos dias 17; 18; 20 e 21 de julho de 2000. A cultivar Fuerte mostrou-se mais tolerante às baixas temperaturas, não apresentando injúrias nas plantas nem queda de frutos; as cultivares híbridas de antilhana x guatemalense apresentaram diferenças de tolerância ao frio; enquanto as antilhanas foram severamente injuriadas.

  6. Cinética de inativação da polifenoloxidase e peroxidase de abacate (Persea americana Mill. Kinetic of inactivation of polyphenoloxidase and peroxidase of avocado (Persea americana Mill.

    Rúbia Caroline Luíz

    2007-12-01

    Full Text Available Extratos enzimáticos foram preparados a partir da polpa de abacate (Persea americana Mill. dos cultivares Quintal, Fortuna e Choquete, em dois estádios de maturação (verde e maduro. A polpa de abacate (150,00g foi homogeneizada em liquidificador, com 300 mL de solução tampão fosfato de sódio 100mM em pH 7,4 para polifenoloxidase (PPO e pH 6,0 para peroxidase (POD. A peroxidase ionicamente ligada foi extraída usando solução de NaCl 1,0M, em tampão fosfato de sódio 100mM pH 6,0. Para o estudo da estabilidade térmica, os extratos foram submetidos a temperaturas de 60, 65, 70, 75 e 80ºC e por períodos que variaram de 1 à 10 minutos e a atividade enzimática foi determinada por espectrofotometria (lambda= 395nm para PPO e lambda= 460nm para POD. Pode-se observar que o declínio rápido da atividade das enzimas era maior nos primeiros quatro minutos e após esse período, com o aumento da temperatura e do tempo, a atividade continuou diminuindo, porém de forma mais lenta. A energia de ativação na faixa de temperatura estudada para as porções termolábil e termorresistente, apresentou valores dentro da faixa 12-100 kcal/mol.Enzymatic extracts were prepared from the avocado pulp (Persea americana Mill., using the cultivars Quintal, Fortuna and Choquete, in two different maturation stages (green and mature avocados. The avocado pulp (150,00g was homogenized in blender with 300 mL of sodium phosphate buffer 100mM at pH 7.4 for polyphenoloxidase (PPO and pH 6.0 for peroxidase (POD. The peroxidase ionically bound was extracted using NaCl solution 1.0M in sodium phosphate buffer 100mM pH 6.0. For the study of the thermal stability the extracts were submitted at temperatures of 60, 65, 70, 75 and 80ºC for periods that had a variation from 1 to 10 minutes and the enzymatic activity was determined using a spectrophotometer (lambda= 395nm for PPO and lambda= 460nm for POD. It was possible to observe a fast decline of the enzyme activity in

  7. Métodos para atrair a abelha Apis mellifera L. em cultura de abacate (Persea americana Mill. Methods to attract honeybee Apis mellifera L. to avocado tree (Persea americana Mill

    Darclet Terezinha Malerbo Souza

    2002-04-01

    Full Text Available O presente experimento teve como objetivo avaliar métodos de atração da abelha Apis mellifera L. (Hymenoptera, Apidae em duas variedades de abacate (Persea americana Mill.. Os atrativos utilizados foram extratos de Cymbopogon citratus, Ocimum basilicum, Lippia alba, folha de Citrus sp, folha de Eucaliptus sp. e o eugenol e o linalol (SIGMA. Os tratamentos utilizados foram: coberto; descoberto pulverizado (DP; descoberto com tubos e descoberto não pulverizado (DNP. Observou-se que a atratividade das substâncias testadas desapareceu minutos após a sua aplicação, utilizando ou não a glicerina, em ambas as variedades. A pulverização dos extratos de falsa melissa, folhas de eucalipto e folhas de laranja apresentaram um aumento no número de abelhas Apis mellifera, na variedade Quintal. Os dados mostraram que a freqüência das abelhas A. mellifera foi maior na variedade Quintal comparada à variedade Fortuna. Isto pode ter ocorrido devido à maior concentração de açúcares do néctar de suas flores. Observou-se que as abelhas A. mellifera preferiram visitar as flores do abacateiro da variedade Quintal, tanto para néctar quanto para coleta de pólen, comparada à variedade Fortuna. Com relação aos frutos, nenhuma das características apresentou diferença significativa entre os tratamentos, em ambas as variedades. Entretanto, observou-se que na variedade Quintal os frutos decorrentes dos tratamentos DP e DNP foram mais pesados, mais compridos e com maior espessura da polpa, comparados ao único fruto obtido do tratamento coberto. Os produtos testados em tubos não foram eficientes para atrair a abelha Apis mellifera, em ambas as variedades.The present experiment was carried out to evaluate some methods to attract honeybee Apis mellifera L. (Hymenoptera, Apidae to two avocado varieties (Persea americana Mill.. Extracts of Cymbopogon citratus, Ocimum basilicum, Lippia alba, Citrus sp leaf, Eucalyptus sp leaf, the eugenol and linalol

  8. Efeito do abacate (Persea americana Mill variedade hass na lipidemia de ratos hipercolesterolêmicos Effect of the hass avocado (American Persea Mill on hipercolesterolemic rats

    Jocelem Mastrodi Salgado

    2008-12-01

    Full Text Available Os fitonutrientes podem ser encontrados em muitas frutas, sendo o abacate o mais rico em beta-sitosterol (fitoesterol e glutationa. O fitoesterol é uma substância de origem vegetal cuja estrutura é muito similar a do colesterol e seu mecanismo de ação envolve a inibição intestinal de absorção do colesterol e diminuição da síntese de colesterol hepático. O efeito é exercido sobre os valores de colesterol plasmático total e LDL, sem afetar os níveis de HDL e triglicérides sanguíneos. O presente trabalho teve como objetivo analisar a influência do consumo de farinha de abacate variedade Hass nos níveis séricos de colesterol total, HDL, LDL, triglicérides, colesterol hepático e excretado. Estes parâmetros foram investigados em ratos Wistar hipercolesterolêmicos durante 30 e 60 dias de experimento. Ao final de 30 dias, a dieta com 15% de abacate mostrou ser mais efetiva para reduzir os níveis de colesterol total e LDL e aumentar o HDL em comparação ao controle. Foi observado que para o teor de colesterol excretado a melhor dieta foi a de 25% de abacate, pois quanto maior a concentração de abacate, maior a excreção de colesterol. A dieta com 15% e 25% de abacate, foram as que mais influenciaram nos níveis de colesterol hepático.Phytonutrients can be found in fruits, and the avocado contains four times more beta-sitosterol (phytosterol and it is one of the best glutathione sources. The phytosterol is a vegetable substance whose structure is very similar to the cholesterol one and its action mechanism involves the intestinal inhibition of cholesterol absorption and the synthesis of hepatic cholesterol. The effect has an influence on the values of total plasmatic cholesterol and LDL without affecting the levels of HDL and triglycerides. The present work had the objective to analyze the influence of the consumption of the Hass avocado on the levels of total cholesterol, HDL, LDL, triglycerides, and hepatic and excreted

  9. Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado seed extracts Composição química, toxicidade, atividade larvicida e antifúngica de extratos de semente de Persea americana (abacate

    João Jaime Giffoni Leite

    2009-04-01

    Full Text Available The present study had the aim of testing the hexane and methanol extracts of avocado seeds, in order to determine their toxicity towards Artemia salina, evaluate their larvicidal activity towards Aedes aegypti and investigate their in vitro antifungal potential against strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis through the microdilution technique. In toxicity tests on Artemia salina, the hexane and methanol extracts from avocado seeds showed LC50 values of 2.37 and 24.13mg mL-1 respectively. Against Aedes aegypti larvae, the LC50 results obtained were 16.7mg mL-1 for hexane extract and 8.87mg mL-1 for methanol extract from avocado seeds. The extracts tested were also active against all the yeast strains tested in vitro, with differing results such that the minimum inhibitory concentration of the hexane extract ranged from 0.625 to 1.25mg L-¹, from 0.312 to 0.625mg mL-1 and from 0.031 to 0.625mg mL-1, for the strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis, respectively. The minimal inhibitory concentration for the methanol extract ranged from 0.125 to 0.625mg mL-1, from 0.08 to 0.156mg mL-1 and from 0.312 to 0.625mg mL-1, for the strains of Candida spp., Cryptococcus neoformans and Malassezia pachydermatis, respectively.O presente estudo teve como objetivo testar os extratos hexânico e metanólico das sementes do abacate, a fim de determinar sua toxicidade em Artemia salina, avaliar a atividade larvicida frente ao Aedes aegypti, bem como verificar o potencial antifúngico in vitro contra cepas de Candida spp, Cryptococcus neoformans e Malassezia pachydermatis, através da técnica de microdiluição. Os extratos hexânico e metanólico das sementes de abacate apresentaram no teste de toxicidade frente à Artemia salina, valores de LC50 2,37 e 24,13mg L-1, respectivamente; contra as larvas do Aedes aegypti os resultados obtidos foram LC50 16,7mg L-1 para o extrato hexânico e 8,87mg

  10. COMPOSIÇÃO DE ÁCIDOS GRAXOS DO ÓLEO DO MESOCARPO E DA SEMENTE DE CULTIVARES DE ABACATE (PERSEA AMERICANA, MILL. DA REGIÃO DE RIBEIRÃO PRETO, SP

    Gisele Lucia MASSAFERA

    2010-11-01

    Full Text Available

    O objetivo deste trabalho foi identificar e determinar os ácidos graxos dos óleos do mesocarpo e da semente de abacates da região de Ribeirão Preto – SP. Para realização do trabalho foram utilizados frutos de abacateiros (Persea americana, Mill. cultivares Fortuna, Ouro Verde e Princesa. O óleo foi obtido através de extração com solvente a quente (Soxhlet e após a esterificação, as amostras foram injetadas em cromatógrafo a gás. No óleo do mesocarpo o ácido graxo que apresentou maior concentração foi o ácido oléico, com teores entre 31,77 e 50,30%, seguido pelo ácido palmítico, com teores entre 20,73 e 32,54%. Os teores de ácidos graxos do óleo do mesocarpo mostraram-se diferentes da semente e variaram entre os cultivares. No óleo do mesocarpo o ácido graxo que apresentou maior concentração foi o ácido oléico, com teores entre 31,77 e 50,30%, seguido pelo ácido palmítico, com teores entre 20,73 e 32,54%. No óleo da semente, o ácido graxo que apresentou maior concentração foi o ácido oléico com teores entre 11,69 e 35,83%, seguido pelo ácido linoléico cujos teores variaram entre 23,28 e 29,98%. No presente estudo pudemos concluir que há uma variabilidade nas concentrações de ácidos graxos entre os diferentes cultivares e também entre a semente e o mesocarpo de cada cultivar.

  11. O óleo de abacate (Persea americana Mill como matéria-prima para a indústria alimentícia The avocado oil (Persea americana Mill as a raw material for the food industry

    Jocelem Mastrodi Salgado

    2008-12-01

    Full Text Available Hábitos de vida saudáveis e uma dieta balanceada aliados ao alto consumo de frutas e vegetais estão associados à redução do risco de doenças e à manutenção da saúde. O óleo de abacate possui em sua composição substâncias bioativas capazes de prevenir e controlar as dislipidemias. Como existem poucas pesquisas científicas avaliando o potencial deste óleo para o consumo humano, o presente trabalho estudou os processos de extração e refino do óleo de abacate. Para tanto, procedeu-se à separação da polpa das outras partes da fruta. A polpa fresca foi seca em estufa, e posteriormente moída para a obtenção de um farelo. O óleo obtido do farelo foi extraído e caracterizado. Os resultados mostraram que os processos de extração e refino do óleo a partir da variedade Margarida são tecnicamente viáveis, o que o torna excelente matéria-prima para a indústria alimentícia. Além disso, possui um perfil de ácidos graxos e esteróis muito semelhante ao perfil do azeite de oliva, podendo desta forma, substituir o óleo de soja e ser utilizado juntamente com o azeite de oliva nos óleos mistos, oferecendo ao consumidor brasileiro um produto de qualidade superior e com menor custo.Healthy life habits and an adequate diet, together with a high intake of fruits and vegetables, are associated with reducing the risk of diseases and maintaining health. The avocado oil has bioactive substances that can prevent and control the hyperlipidemia. Since there is little scientific research evaluating the oil potential for human consumption, the present work studied the extraction and refining process of the avocado oil. Firstly, the pulp was extracted from the fruit. The fresh pulp was dried in a stove and then grounded for obtaining the bran. The oil obtained from de bran was extracted and characterized. The results showed that the extraction and refining process of the Margarida avocado variety is technically feasible, and it is an

  12. The history of anatomy in Persia.

    Shoja, Mohammadali M; Tubbs, R Shane

    2007-04-01

    The study of human anatomy can be found throughout the rich history of Persia. For thousands of years, morphological descriptions derived from this part of the world have contributed to and have helped form our current anatomical knowledge base. In this article we review the major influential Persian periods and the individuals who have contributed to the development of anatomy. We have divided the history of Persia into five eras: (1) the period of the Elamites, Medes, early Persians and Babylonians (10th millennium to 6th century BC); (2) following the establishment of the Persian Empire (6th century BC) to the 7th century AD; (3) after the Islamic conquest of Persia to the ascendency of Baghdad (7th to 13th century AD); (4) from the Mongol invasion of Persia to the foundations of modern anatomy (13th to 18th century AD); and (5) modern Persia/Iran (18th century AD to present). Evidence indicates that human dissection was commonplace in the first era, which led to a disciplined practice of surgery in the centuries leading to the foundation of the Persian Empire. By the emergence of Zoroastrianism in the Persian Empire, the microcosm theory was widely used to understand internal anatomy in relation to the external universe. The world's first cosmopolitan university and hospital were built in Gondishapur, south-western Persia, in the third century AD. Greek and Syriac knowledge influenced the second era. With the gradual ruin of Gondishapur and the foundation of Baghdad following the Islamic conquest of Persia (637-651 AD), a great movement took place, which led to the flourishing of the so-called Middle Age or Islamic Golden Age. Of the influential anatomists of this period, Mesue (777-857 AD), Tabbari (838-870 AD), Rhazes (865-925 AD), Joveini (?-983 AD), Ali ibn Abbas (930-994 AD), Avicenna (980-1037 AD) and Jorjani (1042-1137 AD) all hailed from Persia. There is evidence in the Persian literature as to the direct involvement of these scholars in human

  13. Armazenamento refrigerado de abacates 'Hass'

    Juliana Rodrigues Donadon

    2012-12-01

    Full Text Available Este trabalho objetivou determinar a ocorrência de injúria pelo frio em abacates 'Hass'. Na primeira Etapa, os abacates foram armazenados a 5ºC (77% UR, 10ºC (79% UR e 15ºC (79% UR por até 21 dias. Aos 7; 14 e 21dias de armazenamento, um lote com 36 frutos foi levado ao ambiente (22°C e 77% UR para amadurecimento. Testou-se também o armazenamento a 2°C (74% UR e 4°C (77% UR por até 45 dias, sendo que aos 15; 30 e 45 dias, um lote com a mesma quantidade de frutos foi levado ao ambiente, mantido a 22°C e 77% UR. Frutos armazenados ao ambiente, desde o início, foram considerados testemunha. Avalaram-se a aparência e a luminosidade da polpa. Os frutos armazenados a 10°C e 15°C não apresentaram sintomas de injúrias pelo frio, que foram observados nos frutos armazenados a 2°C, por períodos superiores a 15 dias, e nos mantidos a 4°C, por 30 dias. Quando levados ao ambiente, os frutos armazenados a 2°C conservaram aparência aceitável para o consumo (nota = 3 por até10 dias, e os armazenados a 4°C, por até 12 dias. Na segunda Etapa, analisaram-se a intensidade respiratória e a atividade das enzimas peroxidase (POD, polifenoloxidase (PPO, poligalacturonase (PG e pectinametilesterase (PME nos frutos armazenados a 2ºC, por 45 dias, em comparação com os testemunha. A atividade das enzimas associadas ao escurecimento, POD e PPO, assim como das associadas ao amaciamento da polpa, PME e PG, foi inibida pelo armazenamento a 2ºC, mas readquiriram atividade mais elevada que nos frutos-testemunha, quando foram transferidos ao ambiente, indicando estímulo devido aos danos pelo frio.

  14. The use of Gamma radiation of Cobalt-60 to control avocado moth Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) and its effects on the quality of the fruit of Persea americana (Miller) (Lauraceae).; Uso da radiacao gama do Cobalto-60, para controlar a broca-do-abacate Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) e seus efeitos na qualidade do fruto de Persea americana (Miller) (Lauraceae)

    Silva, Lilian Karla Figueira da

    2004-07-01

    This work aimed to evaluate the influence of Gamma radiation of Cobalt-60, in the avocado moth Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) and its effects on the quality of the fruit Persea americana (Miller) (Lauraceae). For this research, insects were irradiated in ali phases of their life cycle with doses varying from O to 600 Gy and they were maintained at the temperature of 25 +- 2 deg C, humidity of 70 +- 10% and photo phase of 14 h. The species was raised on natural diet, avocado seeds. The cultivar fruits Geada were irradiated with doses that varied from 0 to 150 Gy, maintained for 15 days at room temperature (20 a 35 deg C and humidity of 70 - 80 %) and 30 days at a temperature of 10 deg C, humidity of 40 - 60 %. The chemical-physics and sensorial analyses were carried out. According to the obtained results, it was verified that the lethal dose of gamma radiation to S. catenifer eggs, was of 75 Gy; for caterpillars and pupas was of 300 Gy. The sterile-dose for upcoming adults from irradiated S. catenifer eggs was of 25 Gy; for upcoming adults from irradiated caterpillars, it was of 100 Gy; for adults coming from irradiated pupas was of 150 Gy and for irradiated adults was of 200 Gy. The irradiation in the avocado fruit, maintained at room temperature for 7 days of storage, caused change in the coloration of the fruit (dark spots and yellowish coloration) and more firmness. The sensorial characteristics were kept and the irradiated fruit was the chosen one as favorite for tasting. The irradiated fruits that were kept at 10 deg C, obtained an increase in the storage period, without changing their chemical physics characteristics. The coloration of the fruits was kept, more firmness and a subtle acidity taste increase, being effective in the conservation of the fruits and in the maintenance of their sensorial characteristics. The use of the gamma radiation as treatment quarantine of S. catenifer it was efficient, should be treated them with

  15. Inibição do amadurecimento de abacate com 1-metilciclopropeno

    Kluge Ricardo Alfredo

    2002-01-01

    Full Text Available O abacate (Persea americana é um fruto climatérico que apresenta alta taxa respiratória e alta produção de etileno após a colheita, o que o torna altamente perecível. Por isso, o controle do amadurecimento desse fruto é fundamental para o aumento da vida útil de prateleira. Abacates 'Quintal' foram tratados com o bloqueador da ação do etileno 1-metilciclopropeno (1-MCP nas concentrações de 0, 30, 90 e 270 nL L-1 durante 12 horas a 24masculineC e armazenados sob condições ambientais (24masculineC por nove dias. Diariamente foram analisadas as variáveis coloração da casca e polpa, firmeza de polpa, porcentagem de frutos maduros (firmeza <= 8 Newtons, porcentagem de podridões, taxa respiratória e produção de etileno. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 4 x 10, com quatro repetições de quatro frutos. O 1-MCP reteve o desenvolvimento da coloração da casca e polpa e proporcionou maior firmeza de polpa e menor incidência de podridão nos frutos. Frutos não tratados apresentaram início de amadurecimento após quatro dias a 24masculineC, enquanto nos frutos tratados com 270 nL L-1 de 1-MCP o amadurecimento iniciou após sete dias. Frutos tratados com 1-MCP (270 nL L-1 apresentaram menor taxa respiratória e menor produção de etileno. Os resultados revelam que a aplicação de 1-MCP em abacates é satisfatória para retardar o amadurecimento dos frutos e aumentar a vida de prateleira.

  16. Avicenna's treatise on otology in Medieval Persia.

    Hamidi, Sanaz; Sajjadi, Hamed; Boroujerdi, Amin; Golshahi, Bahare; Djalilian, Hamid R

    2008-12-01

    Avicenna (AD 980-1037) was one of the best-known physicians of his time. Born in Persia, he gained notoriety both in the local and international scenes. Avicenna's best-known book, Canon of Medicine, was the standard textbook of medicine for more than five centuries in Europe and the Persian Empire. Because no English translation of the entire Canon of Medicine exists, the authors have recently translated the treatise on otologic diseases from Persian into English. Correlation was made with the Arabic text as well to ensure accuracy. This study provides a review of Avicenna's significant contributions to the concepts in anatomy and the pathophysiology of ear diseases. Herbal and nonherbal medications used in the treatment of otologic disorders are also discussed.

  17. Determinação enzimática de dopamina em formulações farmacêuticas utilizando sistema de análise por injeção em fluxo com extrato bruto de abacate (Persea americana

    Lupetti Karina Omuro

    2003-01-01

    Full Text Available In this work, a spectrophotometric flow injection analysis system using a crude extract of avocado (Persea americana as a source of polyphenol oxidase to dopamine determination was developed. The substrates and enzyme concentrations from 2.4x10-7 to 5.3x10-4 mol L-1 and 28 to 332 units mL-1 were evaluated, respectively. In addition, the FIA parameters such as sample loop (50 to 500 mL, flow rate (1.4 to 4.3 mL min-1 and reactor length (100 to 500 cm were also evaluated in a 0.1 mol L-1 phosphate buffer solution (pH 7.0. Dopamine solution concentrations were determined using 277 units mL-1 enzyme solution, 400 mL enzyme loop, 375 mL sample loop, 2.2 mL min-1 flow rate and a reactor of 350 cm. The analytical curve showed a linearity from 5.3x10-5 to 5.3x10-4 mol L-1 dopamine with a detection limit of 1.3x10-5 mol L-1. The analytical frequency was 46 h-1 and the RSD lower than 0.5% for 5.3x10-4 mol L-1 dopamine solution (n=10. A paired t-test showed that all results obtained for dopamine in commercial formulations using the proposed flow injection procedure and a spectrophotometric procedure agree at the 95% confidence level.

  18. I fiumi e le acque: i paesaggi fluenti della Persia

    Homa Behbahani

    2015-11-01

    Full Text Available The both grandiose chains of mountains of Persia, the Alborz Mountains, which form a circle round the south shore of Caspian Sea, and the Zagres chain, along the NW-S E direction, look onto the vast Dasht-è-Kavir. Desert. A lot of impressive landscapes, civilizations and human settlements (extraordinary villages and towns were originated along the fluvial valleys, where the snows and the waters sloped down from the mountains flow into the rivers before disappearing in the desert. The ancient Persia is still present and manifest itself in the contemporary landscapes of these remote regions where the populations are still well rooted into such a dynamical and flowing life environment. Here waters, plants and fruits provide the fundamental resources and support sedentary and semi nomad people who live together in this ‘steady and flowing realm’: the first in traditional villages, the second along the valleys. Semi nomad tribes and their animals follow their rhythmic seasonal itineraries and meet every season the sedentary people in their village sharing in peace their common steady and flowing realm. Their history is continuously becoming, weaved by the hands of each woman and by the wisdom of the whole nomad group as a complex marvellous carpet, and it is enriched by the seasonal meetings with the sedentary populations in the villages. At the present this intact wonderful condition is menaced of destruction. A big dam could be built at the end of this valley. 

  19. Qualidade do abacate 'HASS' frigoarmazenado submetido a atmosferas modificadas ativas

    Rogério Lopes Vieites

    2014-06-01

    Full Text Available O objetivo deste trabalho foi avaliar a conservação do abacate 'Hass'frigoarmazenado submetido a atmosferas modificadas ativas. Os frutos foram acondicionados em embalagem de náilon+polietileno e submetidos à injeção de mistura de gases, constituindo os tratamentos: I - mistura gasosa do ambiente (21,0 kPa de O2+0,03 kPa de CO2; II - 4,0 kPa de O2+5,0kPa de CO2 ; III - 4,0 kPa de O2+6,0 kPa de CO2 ; IV - 4,0 kPa de O2+7,0 kPa de CO2, e V- 4,0 kPa de O2+8,0 kPa de CO2. As embalagens contendo os frutos foram armazenadas em câmara frigorífica, a uma temperatura de 10±1ºC e umidade relativa de 90±5%, durante 25 dias, sendo as análises realizadas a cada 5 dias. As análises realizadas foram perda de massa, atividade respiratória, potencial hidrogeniônico (pH, firmeza, acidez titulável (AT, sólidos solúveis (SS e atividade das enzimas pectinametilesterase (PME e poligalacturonase (PG. O tratamento em que os frutos foram submetidos à pressão parcial de 4,0 kPa de O2+7,0 kPa de CO2 foi o mais efetivo na conservação dos frutos, principalmente pela menor perda de massa e baixa produção de CO2.

  20. Armazenamento refrigerado de abacates 'Hass' Cold storage of 'Hass' avocado

    Juliana Rodrigues Donadon

    2012-12-01

    Full Text Available Este trabalho objetivou determinar a ocorrência de injúria pelo frio em abacates 'Hass'. Na primeira Etapa, os abacates foram armazenados a 5ºC (77% UR, 10ºC (79% UR e 15ºC (79% UR por até 21 dias. Aos 7; 14 e 21dias de armazenamento, um lote com 36 frutos foi levado ao ambiente (22°C e 77% UR para amadurecimento. Testou-se também o armazenamento a 2°C (74% UR e 4°C (77% UR por até 45 dias, sendo que aos 15; 30 e 45 dias, um lote com a mesma quantidade de frutos foi levado ao ambiente, mantido a 22°C e 77% UR. Frutos armazenados ao ambiente, desde o início, foram considerados testemunha. Avalaram-se a aparência e a luminosidade da polpa. Os frutos armazenados a 10°C e 15°C não apresentaram sintomas de injúrias pelo frio, que foram observados nos frutos armazenados a 2°C, por períodos superiores a 15 dias, e nos mantidos a 4°C, por 30 dias. Quando levados ao ambiente, os frutos armazenados a 2°C conservaram aparência aceitável para o consumo (nota = 3 por até10 dias, e os armazenados a 4°C, por até 12 dias. Na segunda Etapa, analisaram-se a intensidade respiratória e a atividade das enzimas peroxidase (POD, polifenoloxidase (PPO, poligalacturonase (PG e pectinametilesterase (PME nos frutos armazenados a 2ºC, por 45 dias, em comparação com os testemunha. A atividade das enzimas associadas ao escurecimento, POD e PPO, assim como das associadas ao amaciamento da polpa, PME e PG, foi inibida pelo armazenamento a 2ºC, mas readquiriram atividade mais elevada que nos frutos-testemunha, quando foram transferidos ao ambiente, indicando estímulo devido aos danos pelo frio.This study aimed to determine the occurrence of chilling injury in 'Hass' avocado. In the first step, the avocados were stored at 5ºC (77% RH, 10°C (79% RH, and 15ºC (79% RH for up to 21 days. At 7, 14, and 21 days of storage, one lot with 36 fruits was taken to the environment (22°C and 77% RH for maturation. Storage of fruit at 2°C (74% RH and 4

  1. A Note on the 'Hellenic League against Persia' and the Sanctuary of Zeus at Nemea

    Nielsen, Thomas Heine

    2007-01-01

      The present note discusses why 'The Hellenic League against Persia' made no thank-offering to Zeus at Nemea after its defeat of Xerxes' invasion, even though it did make monumental thank-offerings at the three other great Panhellenic sanctuaries at Delphi, Isthmia and Olympia. It has been argued...... of the neutral stance taken by both Kleonai and Argos during the invasion - which, if accepted, will also mean that the close relations between Kleonai and Argos began prior to 480 BC....

  2. ESTABILIDADE DO GUACAMOLE REFRIGERADO ELABORADO COM ABACATE ‘HASS’ E DIFERENTES FORMULAÇÕES

    Érica Regina DAIUTO

    2012-04-01

    Full Text Available O abacate é um fruto nutritivo com qualidades que viabilizam o aumento de seu consumo. O abacate processado é uma alternativa, mas, um desafio, não somente pelas dificuldades tecnológicas em se manter a qualidade do produto como também agradar o paladar do consumidor ainda não habituado a esta forma de consumo. O objetivo desta pesquisa foi avaliar a estabilidade do guacamole elaborado com diferentes formulações sendo conservado sob refrigeração, para obter um produto com características e qualidade de fresco. O guacamole foi elaborado com abacate ‘Hass’, sendo realizados três tratamentos que diferiram pela adição de limão, ácido ascórbico e ácido cítrico. O produto foi acondicionado em embalagens de nylon+polietileno com e sem aplicação de vácuo e armazenado sob refrigeração (4o C±1. Avaliação dos parâmetros sensoriais, cor, pH e acidez foi realizada aos 0, 3, 7 e 10 dias de armazenamento. A formulação que continha apenas limão foi a mais instável quanto aos parâmetros avaliados durante o armazenamento refrigerado e as amostras permaneceram aceitáveis ao consumo até o sétimo dia. O produto de abacate pode preservar seu aspecto natural e fresco elaborado com ácido cítrico e ascórbico, sendo armazenado sob refrigeração em embalagens de nylon+polietileno.

  3. Characterisation of five candidate genes within the ETEC F4ab/ac candidate region in pigs

    Jacobsen, Mette Juul; Cirera Salicio, Susanna; Joller, David

    2011-01-01

    of possible causative mutations. A total of 34 polymorphisms were identified in either coding regions or their flanking introns. The genotyping data for two of those were found to perfectly match the genotypes at the ETEC F4ab/ac locus, a G to C polymorphism in intron 11 of TFRC and a C to T silent....../ac resistant and ETEC F4ab/ac susceptible animals in any of the tested tissues. CONCLUSIONS: None of the identified polymorphisms are obvious causative mutations for ETEC F4ab/ac susceptibility, as they have no impact on the level of the overall mRNA expression nor predicted to influence the composition...

  4. Emergence of whooping cough: notes from three early epidemics in Persia.

    Aslanabadi, Arash; Ghabili, Kamyar; Shad, Kaveh; Khalili, Majid; Sajadi, Mohammad M

    2015-12-01

    Whooping cough is a relatively new infectious disease afflicting human beings, compared with other infectious diseases, and is undergoing a resurgence despite decades of vaccination. The oldest known epidemic is thought to be the Paris outbreak of 1578. In this Historical Review, we describe three epidemics of whooping cough in Persia, which although arising roughly one century before the Paris outbreak, have not been examined in detail. A great amount of epidemiological detail was reported that not only distinguishes the various stages and complications of whooping cough, but also reveals unique immunological aspects of this disease. The first of these epidemics is the oldest recorded whooping cough epidemic. On the basis of epidemiological features, we propose that this whooping cough epidemic was the first to have taken place in Persia and might have been part of the first pandemic. This theory pushes back the date of first documented emergence of whooping cough by almost a century, which matches molecular data about its spread. Here, we discuss features of these early epidemics in relation to their initial emergence, potential origins, and spread to Europe.

  5. Sleep paralysis in medieval Persia – the Hidayat of Akhawayni (? –983 AD

    Golzari SE

    2012-06-01

    Full Text Available Samad EJ Golzari,1 Kazem Khodadoust,5 Farid Alakbarli,6 Kamyar Ghabili,2 Ziba Islambulchilar,3 Mohammadali M Shoja,1 Majid Khalili,1 Feridoon Abbasnejad,1 Niloufar Sheikholeslamzadeh,7 Nasrollah Moghaddam Shahabi,4 Seyed Fazel Hosseini,2 Khalil Ansarin11Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences; 2Medical Philosophy and History Research Center, Tabriz University of Medical Sciences; 3Department of Pharmaceutics, Tabriz University of Medical Sciences; 4Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 5Azerbaijan National Academy of Sciences; 6Institute of Manuscripts of the Azerbaijan National Academy of Sciences, Baku, Azerbaijan; 7Faculty of Law, Central Tehran Branch, Islamic Azad University, Tehran, IranAbstract: Among the first three manuscripts written in Persian, Akhawayni's Hidayat al-muta`allemin fi al-tibb was the most significant work compiled in the 10th century. Along with the hundreds of chapters on hygiene, anatomy, physiology, symptoms and treatments of the diseases of various organs, there is a chapter on sleep paralysis (night-mare prior to description and treatment of epilepsy. The present article is a review of the Akhawayni's teachings on sleep paralysis and of descriptions and treatments of sleep paralysis by the Greek, medieval, and Renaissance scholars. Akhawayni's descriptions along with other early writings provide insight into sleep paralysis during the Middle Ages in general and in Persia in particular.Keywords: sleep paralysis, night-mare, Akhawayni, Persia

  6. Agave Americana Leaf Fibers

    Ashish Hulle

    2015-02-01

    Full Text Available The growing environmental problems, the problem of waste disposal and the depletion of non-renewable resources have stimulated the use of green materials compatible with the environment to reduce environmental impacts. Therefore, there is a need to design products by using natural resources. Natural fibers seem to be a good alternative since they are abundantly available and there are a number of possibilities to use all the components of a fiber-yielding crop; one such fiber-yielding plant is Agave Americana. The leaves of this plant yield fibers and all the parts of this plant can be utilized in many applications. The “zero-waste” utilization of the plant would enable its production and processing to be translated into a viable and sustainable industry. Agave Americana fibers are characterized by low density, high tenacity and high moisture absorbency in comparison with other leaf fibers. These fibers are long and biodegradable. Therefore, we can look this fiber as a sustainable resource for manufacturing and technical applications. Detailed discussion is carried out on extraction, characterization and applications of Agave Americana fiber in this paper.

  7. [Codependence in Péter Hajnóczy's novella Death rode out of Persia].

    Marjai, Kamilla

    2015-01-01

    It is assumed that in connection with literature the artistic product generates interaction between the creator and the recipient, as in the all other cases of art. The formation of a writing leads to the birth of the reading, while the reader creates a new quality weaving artistic literature into a subjective context, this is how the so-called "personal reading" springs into existence. The Death rode out of Persia (A halal kilovagolt Perzsiabol) written by Peter Hajnoczy - which is about the everyday life of a man suffering from alcoholism -facilitates not only the formation of a subjective reading based on emotional impressions, but also provokes the formation of an analytic reading which reflects on the phenomena of addiction and the loss of control. This kind of focused analysis claims to let the particular art product and the professional literature of addictology meet on the field of alcoholism and codependence.

  8. Sleep paralysis in medieval Persia - the Hidayat of Akhawayni (?-983 AD).

    Golzari, Samad Ej; Khodadoust, Kazem; Alakbarli, Farid; Ghabili, Kamyar; Islambulchilar, Ziba; Shoja, Mohammadali M; Khalili, Majid; Abbasnejad, Feridoon; Sheikholeslamzadeh, Niloufar; Shahabi, Nasrollah Moghaddam; Hosseini, Seyed Fazel; Ansarin, Khalil

    2012-01-01

    Among the first three manuscripts written in Persian, Akhawayni's Hidayat al-muta'allemin fi al-tibb was the most significant work compiled in the 10th century. Along with the hundreds of chapters on hygiene, anatomy, physiology, symptoms and treatments of the diseases of various organs, there is a chapter on sleep paralysis (night-mare) prior to description and treatment of epilepsy. The present article is a review of the Akhawayni's teachings on sleep paralysis and of descriptions and treatments of sleep paralysis by the Greek, medieval, and Renaissance scholars. Akhawayni's descriptions along with other early writings provide insight into sleep paralysis during the Middle Ages in general and in Persia in particular.

  9. Characterisation of five candidate genes within the ETEC F4ab/ac candidate region in pigs

    Bertschinger Hans U

    2011-06-01

    Full Text Available Abstract Background Enterotoxigenic Escherichia coli (ETEC that express the F4ab and F4ac fimbriae is a major contributor to diarrhoea outbreaks in the pig breeding industry, infecting both newborn and weaned piglets. Some pigs are resistant to this infection, and susceptibility is inherited as a simple dominant Mendelian trait. Indentifying the genetics behind this trait will greatly benefit pig welfare as well as the pig breeding industry by providing an opportunity to select against genetically susceptible animals, thereby reducing the number of diarrhoea outbreaks. The trait has recently been mapped by haplotype sharing to a 2.5 Mb region on pig chromosome 13, a region containing 18 annotated genes. Findings The coding regions of five candidate genes for susceptibility to ETEC F4ab/ac infection (TFRC, ACK1, MUC20, MUC4 and KIAA0226, all located in the 2.5 Mb region, were investigated for the presence of possible causative mutations. A total of 34 polymorphisms were identified in either coding regions or their flanking introns. The genotyping data for two of those were found to perfectly match the genotypes at the ETEC F4ab/ac locus, a G to C polymorphism in intron 11 of TFRC and a C to T silent polymorphism in exon 22 of KIAA0226. Transcriptional profiles of the five genes were investigated in a porcine tissue panel including various intestinal tissues. All five genes were expressed in intestinal tissues at different levels but none of the genes were found differentially expressed between ETEC F4ab/ac resistant and ETEC F4ab/ac susceptible animals in any of the tested tissues. Conclusions None of the identified polymorphisms are obvious causative mutations for ETEC F4ab/ac susceptibility, as they have no impact on the level of the overall mRNA expression nor predicted to influence the composition of the amino acids composition. However, we cannot exclude that the five tested genes are bona fide candidate genes for susceptibility to ETEC F4ab/ac

  10. The first outbreak of brucellosis in the region of Šabac

    Marković-Denić Ljiljana

    2010-01-01

    Full Text Available Background/Aim. In Serbia brucellosis is a primary disease of the animals in the southern parts of the country. The aim of this study was to describe the first outbreak of human and animal brucellosis in the region of Šabac, Serbia. Methods. An epidemiological investigation was conducted to identify a source of outbreak and the ways of transmission of brucellosis infection in human population. A descriptive and analytical epidemiological methods (cohort study were used. Additional data included monthly reports of the infectious diseases from the Institutes of Public Health and data from the Veterinary Specialistic Institute in Šabac. The serological tests for human brucellosis cases were performed in the Laboratory of the Military Medical Academy; laboratory confirmation of animal brucellosis cases was obtained from the reference laboratory of the Faculty of Veterinary Medicine, Belgrade. Results. Twelve cases of brucellosis were recorded from February 9 to September 1, 2004. Total attack rate was 8.1% (7.5% of males, 14.2% of females. Relative risk (RR of milk consumption was 8.9 (95% confidence interval: 1.63-13.38, and RR for direct contact with animals was 14 (95% confidence interval: 3.5-55.6. The prevalence of seropositive animals in 33 villages of the Mačva region accounted for 0.8%. Regarding animal species, sheep were predominant - 264 (95.7%. Out of a total number of seropositive animals, ELISA results were positive in 228 (88.7% of them. Conclusion. As contact epidemics generally last longer, it is probable that the implemented measures of outbreak control did reduce the length of their duration.

  11. Leishmaniose tegumentar americana

    Gontijo Bernardo

    2003-01-01

    Full Text Available A leishmaniose tegumentar americana permanence endêmica em vastas áreas da América Latina. Os agentes causadores da doença são a L. (Viannia braziliensis, L. (L. mexicana, L. (V. panamensis, e outras espécies relacionadas. A apresentação clínica da doença varia dentro de um espectro amplo, incluindo úlceras cutâneas múltiplas ou única, leishmaniose cutânea difusa e lesões mucosas. Os principais reservatórios da L. (V. braziliensis e da L. (Viannia spp. são os pequenos roedores silvestres. A doença acomete mais freqüentemente os trabalhadores que invadem as florestas tropicais ou moram próximo a elas. O período de incubação varia de duas semanas a vários meses. As lesões cutâneas constituem úlceras rasas, circulares com bordas elevadas e bem definidas e com o assoalho da úlcera de aspecto granular. Nas infecções pela L. (V. braziliensis a linfoadenopatia regional geralmente precede o surgimento das úlcerações por uma a doze semanas. O diagnóstico definitivo depende da identificação de amastigotas em tecido ou promastigotas em meios de cultura. Os anticorpos anti-leishmania podem ser identificados no soro utilizando-se as técnicas de ELISA, imunofluorescência e testes de aglutinação mas os títulos revelam-se baixos na maioria dos casos. A intradermorreação de Montenegro torna-se positiva durante a evolução da doença. Os antimoniais pentavalentes continuam sendo as drogas de escolha no tratamento da leishmaniose. A anfotericina B encontra indicação nos casos mais graves ou nos indivíduos que não respondem ao tratamento com os antimoniais. A imunoterapia e a imunoprofilaxia constituem alternativas promissoras no tratamento e profilaxia da leishmaniose tegumentar americana.

  12. Capacidade antioxidante e qualidade pós-colheita de abacate 'Fuerte'

    Rogério Lopes Vieites

    2012-06-01

    Full Text Available A capacidade antioxidante e a qualidade pós-colheita do abacate 'Fuerte' foram avaliadas. Os frutos foram mantidos sob temperatura ambiente (24 ± 1 ºC e sob refrigeração em 10 ± 1 ºC e 90±5% UR e avaliados durante 15 dias. Determinaram-se a perda de massa e a taxa respiratória. As características físico-químicas avaliadas foram acidez total titulável (AT, sólidos solúveis (SS, ratio, pH, firmeza, atividade da enzima polifenoloxidase (PPO, cor (L, a* e b*, fenólicos totais e atividade antioxidante (AA por DPPH·. Nas duas condições de armazenamento, o pico respiratório ocorreu no 9º dia.Os frutos mantidos sob temperatura refrigerada apresentaram menor perda de massa e maior firmeza em relação àqueles em temperatura ambiente. O comportamento respiratório refletiu na redução dos valores de AT e no conteúdo de SS que diminuíram a partir do pico de respiração. Os valores de pH aumentaram como consequência da diminuição da AT. Os valores de luminosidade mantiveram-se elevados, observando-se diminuição do componente de cor verde na polpa com o armazenamento. A atividade da PPO diminuiu com o armazenamento. Os valores de AA variaram de 17,6 a 68,7%. O conteúdo de fenólicos totais diminuiu após o pico respiratório, e valores crescentes de AA foram observados mesmo após o 9º dia.

  13. Polymorphisms of three gene-derived STS on Pig chromosome 13q41 are associated with susceptibility to enterotoxigenic Escherichia coli F4ab/ac in pigs

    HUANG Xiang; REN Jun; YAN XueMing; PENG QiuLing; TANG Huan; ZHANG Bo; JI HuaYuan; YANG ShuJin; HUANG LuSheng

    2008-01-01

    Neonatal diarrhea caused by enterotoxigenic Escherichia coli (ETEC) F4 is a common and serious disease, resulting in significant economical loss in the pig industry. The locus encoding ETEC F4 re-ceptor has been mapped to pig chromosome (SSC) 13q41, and one of the most significantly linked markers is S0075. In this study, we selected three genes including SLC12A8, MYLKand KPNA1 from a chromosomal region flanking S0075 on SSC13 to develop pig specific sequence tagged sites (STS). Seven single nucleotide polymorphisms were identified in the three pig STS using DNA of four full-sib susceptible and resistant animals in a White Duroc × Erhualian intercross. All grandparents, parents and 755 offspring in the intercross were genotyped for three polymorphisms, including SLC12A8 g.159AG, MYLK g.1673AG and KPNA1g.306AG. Family-based transmission disequilibrium test (TDT) revealed that.all polymorphisms and the corresponding haplotypes are significantly associated with ETEC F4ab/ac (especially F4ac) brush border adhesion phenotypes, indicating that these polymor-phism are in linkage disequlibrium with causal mutation(s) of the gene encoding ETEC F4ab/ac receptor. Our results strengthen the evidence for the involvement of SSC13q41 in high acquiring risk of ETEC F4ab/ac infection, and provide novel .polymorphic markers for fine mapping of the ETEC F4ab/ac re-ceptor locus.

  14. [Contact dermatitis from Agave americana].

    de la Cueva, Pablo; González-Carrascosa, Mateo; Campos, Minia; Leis, Vicente; Suárez, Ricardo; Lázaro, Pablo

    2005-10-01

    Numerous plant species and their derivatives can cause skin reactions through a variety of mechanisms: irritative contact dermatitis, allergic contact dermatitis, contact urticaria and photodermatitis. We present a case of irritative contact dermatitis after exposure to the sap of Agave americana. The skin symptoms in this case have only been described on rare occasions; although this condition usually presents with a papulovesicular rash, in this patient it appeared as purpuric lesions in the contact area.

  15. Ewingella Americana: An Emerging True Pathogen

    Syed Hassan

    2012-01-01

    Full Text Available Infections caused by Ewingella americana have been rarely reported in the literature. Most of the cases that have been reported were among the immunocompromised patients. We report a case of E. americana causing osteomyelitis and septic arthritis of the shoulder joint in a previous intravenous drug abuser. The causative pathogen was identified by synovial fluid analysis and culture.

  16. ABAC策略语义表示和决策方法%Semantic representation and enforcement methods of ABAC policies

    周加根; 叶春晓; 罗娟

    2013-01-01

    To solve the semantic presentation and enforcement problems of ABAC policies in the open system environment, a method using ontology to define policies is proposed. This method is defined on the basis of a map from ABAC policy model to description logic definitions. Also, it uses SWRL rules to define relations in the system. Based on the policy ontology, a frame-work utilizing close world reasoning and individual realization reasoning service to generate decisions of access request is pro-posed. The correctness of policy enforcement method is proved through its soundness and completeness, and an experiment is showed to verify the feasibility of these methods in a real application.%为解决开放式系统环境中基于属性的访问控制(Attribute-Based Access Control,ABAC)策略语义层次上的表示和决策问题,提出了ABAC策略的本体表示方法。该方法基于ABAC策略模型到描述逻辑定义的映射,使用语义Web规则语言(SWRL)处理系统内部关系定义。在此基础上,提出了基于封闭世界和实例实现推理的策略决策框架。最后从可靠性和完备性两方面说明了决策方法的正确性,验证实验表明了方法在实际应用中的适用性。

  17. Los efectivos del último ejército de la Persia Sasánida (572-642. Una solución desde las fuentes

    Soto Chica, José

    2015-12-01

    Full Text Available The question of the number of troops that existed in the Sassanids’ armies during the 6th and 7th century has received various answers. Answers that, paradoxically, have not taken into account the specific and multiple information that provide the sources of this period. From our side in this paper, we address this question taking into account all the sources, whether these are Byzantine, Oriental, Persian or Perso-Islamic, and we try to show that the careful study of the data and the critical comparison permits us to evaluate with extreme precision the military power of the Sassanian Persia and to establish a comparative basis to shed new light on the late conflicts between Sassanid Persia and the Justinian’s or Heraclius’ Byzantium as well as those with the Arab armies of early Islam.La cuestión del número de efectivos con que contaron los ejércitos sasánidas de los siglos VI y VII ha recibido diferentes respuestas. Respuestas que, paradójicamente, no han tenido en cuenta las precisas y múltiples informaciones que al respecto proporcionan las fuentes del periodo. Por nuestra parte y en este trabajo, abordamos dicha cuestión teniendo en cuenta la totalidad de las fuentes, ya sean estas bizantinas, orientales, persas o persoislámicas y tratamos de mostrar que el atento estudio de los datos y su confrontación crítica, permite evaluar con suma precisión el poderío militar de la Persia sasánida y establecer así una base comparativa que arroje nueva luz sobre los últimos conflictos de la Persia sasánida con el Bizancio Justinianeo y Heraclida y con los ejércitos árabes del primer Islam.

  18. Marker-assisted selection on E. coli F4ab/ac resistance and the effect on neonatal survival in piglets

    Jørgensen, Claus Bøttcher; Anderson, Susan I.; Cirera, Susanna;

    Enterotoxigenic Escherichia coli (ETEC) that express the F4ab or F4ac fimbriae (formerly known as K88ab/ac) are major causes of diarrhea and death in neonatal and young pigs. A locus controlling susceptibility towards ETEC F4ab/ac has previously been mapped to pig chromosome 13q41. A number...... to the genomic sequence of pig mucin 4 we also report on more than 15 SNPs discovered in the porcine MUC4 sequence. Collaboration with the Danish Slaughterhouses on genotyping of the intron 7 SNP of Mucin 4 in 6.373 breeding boars (3.432 Landrace and 2.941 Large White) and analysis of their offspring has...... revealed data of relevance to neonatal survival. Analysis on offspring from 6.373 animals show a highly significant (P

  19. Polifenoles en Myristicaceae americanas (I Polifenoles en Myristicaceae americanas (I

    Aguirre Gálviz Luis Enrique

    1988-06-01

    Full Text Available

    The ocurrence and distribution of phenolic acids and flovonoid agIycones in 122 samples from 22 species of American Myristicaceae, other than Colombian, were studied. The samples were obtained from herbarium specimens in order to establish a qualitative comparison against fresh samples, pointing out to the possibility of studying such compounds directly on botanical voucher specimens for the purpose of phytochemical and cbemmotaxonomic discussions.

    Se estudió1a distribución de agliconas de ácidos fenólicos y flavonoides en 122 muestras de 22 especies de Myristicaceae americanas no colombianas. Las muestras eran provenientes de especímenes de herbario, para comparación cualitativa con muestras frescas, indicando la utilidad de estudios de tales compuestos realizados directamente sobre especímenes botánicos en investigaciones quimiotaxonómicas y fotoquímicas.

  20. STUDY ON ACCESS CONTROL FOR WEB SERVICES BASED ON ABAC%基于ABAC的Web Services访问控制研究

    夏春涛; 杨艳丽; 曹利峰

    2012-01-01

    为解决Web Services访问控制问题,分析了传统访问控制模型在Web Services应用中的不足,给出了面向Web Services 的基于属性的访问控制模型ABAC(Attribute Based Access Control)的定义,设计了ABAC访问控制架构,并利用可扩展的访问控制标记语言XACML( eXtensible Access Control Markup Language)实现了细粒度的Web Services访问控制系统.系统的应用有效保护了Web Services资源.%To deal with access control for web services, the problem of application of traditional access control model in web services is analysed, then the definition of web services-oriented attribute-based access control ( ABAC) model is presented, and the architecture of ABAC is designed. Furthermore, the fine-grained access control system for web services is implemented with XACML, the application of the system has effectively protected the resources of web services.

  1. ELABORAÇÃO, COMPOSIÇÃO QUÍMICA E AVALIAÇÃO SENSORIAL DE SOBREMESAS LÁCTEAS ACHOCOLATADAS COM ABACATE

    Nathalia SOLER

    2011-01-01

    Full Text Available O aproveitamento de frutas tropicais na elaboração de produtos lácteos tem sido estudado no intuito da valorização destes e na tentativa de agregar maior valor nutricional a tais preparações. O objetivo deste trabalho foi desenvolver sobremesas lácteas achocolatadas (SLA com diferentes proporções de abacate e determinar a sua composição centesimal e de compostos antioxidantes, além de veri- fi car a aceitação e atributos destas formulações. Elaboraramse três formulações contendo 28,8% de abacate (FA, 36,3% (FB, e 41,7% (FC. Para o preparo das formulações utilizouse como ingredientes: abacate, açúcar, achocolatado, creme de leite e leite. As análises químicas realizadas foram: composição centesimal, determinação de fenólicos, fl avonoides e antocianinas totais. Os testes sensoriais compreenderam: teste de aceitação com escala hedônica de 9 pontos; teste de preferência por ordenação, e análise de atributos sensoriais. Na composição nutricional a FC apresentou os maiores teores de lipídios, fl avonoides e antocianinas em relação às demais (p<0,05. FA e FB obtiveram as maiores notas no teste de aceitação (7,26 e 6,57 respectivamente e a FA recebeu as maiores médias de preferência (p<0,05. A formulação FA recebeu as maiores notas nos atributos: cor, brilho, aparência, cheiro/aroma e textura. A inclusão de abacate nas SLA promoveu o aumento dos componentes químicos avaliados, principalmente de lipídios e substâncias antioxidantes. Além disso, as SLA podem ser elaboradas com até 36,3% de abacate, pois possuem boa aceitação, e atributos sensoriais favoráveis ao seu consumo.

  2. Linkage and comparative mapping of the locus controlling susceptibility towards E. COLI F4ab/ac diarrhoea in pigs.

    Jørgensen, C B; Cirera, S; Anderson, S I; Archibald, A L; Raudsepp, T; Chowdhary, B; Edfors-Lilja, I; Andersson, L; Fredholm, M

    2003-01-01

    In 1995, Edfors-Lilja and coworkers mapped the locus for the E. COLI K88ab (F4ab) and K88ac (F4ac) intestinal receptor to pig chromosome 13 (SSC13). Using the same family material we have refined the map position to a region between the microsatellite markers Sw207 and Sw225. Primers from these markers were used to screen a pig BAC library and the positive clones were used for fluorescent in situ hybridization (FISH) analysis. The results of the FISH analysis helped to propose a candidate gene region in the SSC13q41-->q44 interval. Shotgun sequencing of the FISH-mapped BAC clones revealed that the candidate region contains an evolutionary breakpoint between human and pig. In order to further characterise the rearrangements between SSC13 and human chromosome 3 (HSA3), detailed gene mapping of SSC13 was carried out. Based on this mapping data we have constructed a detailed comparative map between SSC13 and HSA3. Two candidate regions on human chromosome 3 have been identified that are likely to harbour the human homologue of the gene responsible for susceptibility towards E. COLI F4ab/ac diarrhoea in pigs.

  3. Caracterização da agressividade e atividade enzimática de isolados de Colletotrichum spp. associados à antracnose do abacate

    Hugo José Tozze Júnior

    Full Text Available RESUMO A antracnose é a principal doença pós-colheita do abacate. O presente trabalho teve como objetivos caracterizar a agressividade e a atividade enzimática de 30 isolados de Colletotrichum spp. de abacate. Abacates ‘Fuerte’ foram inoculados com disco de BDA contendo estruturas de cada isolado e após sete dias mensuraram-se os diâmetros das lesões. Dois grupos de isolados foram distinguidos quanto à agressividade pelo teste de teste de Scott-Knott (p=0,05, um com lesões entre 34,0 e 38,2 mm e outro com lesões entre 38,7 e 44,0 mm de diâmetro. Para caracterização enzimática, avaliaram-se as áreas dos halos formados em substratos específicos para detecção de amilase, celulase, lacase, lipase, pectato liase e protease e a atividade da enzima catalase. Os isolados não produziram amilase suficiente para formação de halo em meio de cultura. Todos os isolados produziram catalase, pectato liase e protease, enquanto 46% produziram lacase e 97% tiveram as atividades da celulase e lipase detectadas. Distinguiram-se dois grupos de isolados para as enzimas catalase e pectato liase, cinco grupos para a celulase, seis grupos para a lacase e sete grupos para as enzimas lipase e protease, pelo teste de teste de Scott-Knott (p=0,05. Não houve relação entre as atividades enzimáticas e a agressividade dos isolados.

  4. Métodos de proteção de mudas de abacate contra geada em diferentes cultivares Frost protection covers methods for avocado in different cultivars

    Ubirajara Ribeiro Mindêllo Neto

    2004-08-01

    Full Text Available Este trabalho foi realizado na EMBRAPA Transferência de Tecnologia/SNT, localizado em Canoinhas-SC, Planalto Norte Catarinense. O objetivo foi avaliar a tolerância ao frio de oito cultivares de abacate (Choquette, Vitória, Herculano, Quintal, Linda, Tonnage, Fuerte e Ryan plantadas em maio de 2003, utilizando dois métodos de proteção da muda contra geadas (galhos de eucalipto e galhos de eucalipto + sombrite. Em setembro do mesmo ano, estes materiais que protegiam as mudas, foram retirados e foram atribuídas notas para avaliar o grau de dano nas plantas. Não houve diferença entre as cultivares de abacate em relação à tolerância ao frio, independentemente do tipo de proteção. Entretanto, comparados os dois métodos avaliados, a utilização de galhos de eucalipto associado ao uso de sombrite foi mais eficiente na proteção de plantas jovens de abacate quando comparado ao uso de galhos de eucalipto.The experiment was carried out at EMBRAPA Technology Transfer Office at Canoinhas, Santa Catarina State, Northern Highland area. The aim was to evaluate the tolerance for low temperature in eight varieties of avocado (Choquette, Vitória, Herculano, Quintal, Linda, Tonnage, Fuerte and Ryan planted in May of 2003 using two methods of covers for avocado maiden trees (eucalyptus branches and eucalyptus branches plus sombrite. In September, in the same year, the covers were removed and ranked by scores to evaluate the degree of frost injury on plants. There were no difference between avocado varieties in relation of covers methods. However, comparing the two covers methods, the use of eucalyptus branches plus sombrite was better than the use of eucalyptus branches, in young plants of avocado.

  5. [Dermatitis from contact with Agave americana].

    Golan, H; Landau, M; Goldberg, I; Brenner, S

    2000-10-01

    Various plants induce dermatitis in man. There have been only a few published cases of contact dermatitis caused by Agave americana (AA). We report intentional exposure to AA in a soldier seeking sick leave, and review our previously reported cases. Treatment with oral antihistamines and topical saline compresses resulted in subsidence of the systemic symptoms within 24 h and regression of cutaneous manifestations in 7-10 days. Physicians should be alert to the possibility of self-inflicted contact dermatitis induced by exposure to plants, especially to A. americana. Systemic signs may accompany the cutaneous lesions.

  6. Caracterización de algunos rasgos vegetativos y reproductivos en variedades de aguacate, Persea americana Mill.

    Escandón Gil Gonzalo

    1989-12-01

    Full Text Available En Palmira (Colombia entre 1984 y 1988 se caracterizó la colección de aguacate, Persia americana Mili. Se halló la ecuación de regresión AF = 89.25 + 0.60 (La -144, R2= 98.58 %, que permite estimar el área foliar (AF con el producto largo por ancho de las hojas (A. Las variedades Mayapan, Oriente 1, Oriente 3, Simonds y los materiales 2, 3, 4, 5 y 6 presentaron épocas de floración definidas, Trinidad y Material 2 presentaron floraciones bajas pero continuas. El porcentaje de flores que llegó a frutos osciló entre 0.03 % para Oriente 3 y 0.57 % para Trinidad. La mayor pérdida de estructuras reproductivas ocurrió en los primeros días de floración. El modelo logístico no describió correctamente el desarrollo de los frutos, por esto se utilizó el modelo de línea recta por fases. Los materiales 2 y 5 presentaron 105mayores pesos de frutos (621 y 626 g; el de menor peso fue Booth 8. La pérdida de peso del fruto durante la maduración fluctuó entre 3.5 Y 12.8 % para el material 2 y Oriente 3 respectivamente. Los materiales de mayor aceptación por la presentación del fruto y sabor fueron los materiales 1, 2 y 5.A collection of avocado, Persea americana Mill, was characterized in Palmira (Colombia between1984 and 1988. It was found that regretion equation AF = 89.25 + 0.60 (LA-144, R2 = 98.58 %, allows to estimate the foliar area with the product length x width of the leaves. The Mayapan, Oriente 1, Oriente 3, Simmonds and Materia.1 2,3,4,5,6, varieties showed defined blooming time, Trinidad and Material 2 showed low and continue blooming times. The percentajes of flowers that matured in fruit fluctuated between 0.03 % of Oriente 3 and 0.57 % for Trinidad. The greater loss proportion of reproductive structures occurred during the first days of flowering. The logistic model did not describe the right developments of fruits, that is why a straight line model by stages was used. Material 2 and 5 showed the greatest fruit weights during

  7. Constituents of Agave americana and Agave barbadensis.

    Tinto, W F; Simmons-Boyce, J L; McLean, S; Reynolds, W F

    2005-09-01

    An investigation of Agave americana and Agave barbadensis resulted in the isolation of a new homoisoflavanoid, 7-hydroxy-3-(4-methoxybenzyl)-chroman (3), together with known compounds 7-hydroxy-3-(4-methoxybenzyl)-chroman-4-one (1), 5,7-dihydroxy-3-(4-methoxybenzyl)-chroman-4-one (2), cantalasaponin-1 (4), and 2-hydroxy-butanedioic acid-1-methyl ester (5).

  8. Mechanism and control of Genipa americana seed germination

    Queiroz, S.E.E.; Silva, da E.A.A.; Davide, A.C.; Jose, A.C.; Silva, A.T.; Fraiz, A.C.R.; Faria, J.M.R.; Hilhorst, H.W.M.

    2012-01-01

    Genipa americana (Rubiaceae) is important for restoration of riparian forest in the Brazilian Cerrado. The objective was to characterize the mechanism and control of germination of G. americana to support uniform seedling production. Morphology and morphometrics of seeds, embryo and endosperm were a

  9. Food and Migration: the Abacería Central Market of Gràcia (Barcelona Le Marché Central de l’Abaceria à Gracia (Barcelone: mixages et métissages des goûts

    F.Xavier Medina

    2010-12-01

    Full Text Available Local food markets are, aside from being places for provisioning, spaces for meeting and social interaction. This article engages in an analysis of the social and commercial relations exhibited in the geographical area of the Abacería Central Market, in the neighborhood of Gràcia (Barcelona: a neighborhood that, over the last two decades, has seen a significant rise in the number of residents arriving from other countries (primarily Latin America, Africa and Eastern Europe. The Abacería Central Market and the synergy created among the different market stalls and businesses in the market’s immediate vicinity (whether these are ethnic or not define a material and social space in the neighborhood that is key for understanding the social relations that are established at the local level.Les marchés locaux d'alimentation sont des espaces de rencontre et d'interaction sociale, outre leur fonction d'approvisionnement. Cet article propose une analyse des liens sociaux et commerciaux qui se déploient dans l'enceinte du marché de l'Abacería, dans le quartier barcelonais de Gracia; c'est un quartier qui, durant les deux dernières décennies, a connu une augmentation significative du nombre de résidents en provenance principalement d'Amérique latine, d' Afrique et d'Europe de l'Est. Le marché central de l'Abacería établit des synergies entre les différent étals, ainsi qu'avec les commerces avoisinants (de caractère ethnique ou non qui définissent le quartier comme espace matériel et social et s'avèrent être fondamentaux pour comprendre comment s'agencent les rapports sociaux, à l'échelon local.

  10. Infrared thermography applied to rhea (Rhea americana

    Jânio Lopes Torquato

    2015-04-01

    Full Text Available Infrared thermography is an innovative tool that has been used in several studies involving animal. This tool used to study heat stress, thermoregulation, behavior, disease diagnosis, mainly in the animal production and animal welfare. Wild birds are animals that get tense with the presence of man in his environment, causing changes in its thermal physiology and behavior. In the Brazilian semiarid, high temperatures cause further stress the birds, in studies involving animal welfare and animal production is important to use procedures and tools that do not cause stress to the animal, hence the implementation of infrared thermography. The Rhea americana is a wild bird, native to Brazil, which is gaining market share because of its meat is healthy and other products that are extracted from it. Little is known about the thermal physiology and behavior of this ratita, it is essential to improve poultry facilities in order to provide a pleasant environment and increase production. Birds and other ratites as the ostrich, emu, kiwi and cassowary use some body areas to dissipate body heat, the areas are: legs, beak and neck. All these body areas used by birds to dissipate heat were found using infrared thermography. Being a ratita, Rhea Americana can use the same body regions to reduce heat stress. So, infrared thermography is critical to the thermoregulatory studies of rheas in hot environments.

  11. Nota Científica: Caracterização físico-química do óleo de abacate extraído por centrifugação e dos subprodutos do processamento

    Roseli Aparecida FERRARI

    2015-03-01

    Full Text Available O abacate é originário da América e considerado uma das plantas mais produtivas por unidade de área cultivada. Frutas com altos níveis de lipídios podem ser matéria-prima importante para a extração de óleo com substâncias bioativas que podem reduzir o risco de doenças. Este trabalho teve como objetivo a extração de óleo de abacate utilizando processo mecânico, semelhante ao empregado no azeite de oliva. À polpa triturada foi adicionada água numa proporção de 1:1, e a mistura foi mantida durante 40-60 minutos a 45-50 °C. Óleo, água e sólidos foram separados por centrifuga de três fases e o óleo filtrado. A composição físico-química do abacate e dos subprodutos do processamento foi determinada utilizando a metodologia oficial da AOCS e constou da determinação dos teores de proteínas, umidade, cinzas e lipídios (óleo. A caracterização de ácidos graxos do óleo de abacate foi determinada por cromatografia em fase gasosa. Os índices de saponificação e de iodo do óleo de abacate foram calculados com base na composição em ácidos graxos. O óleo apresentou entre 0,5 e 0,6% de ácidos graxos livres; 5,95 a 11,89 meq kg–1 de peróxidos; 0,09-0,16% de umidade; 0,96-1,94% de matéria insaponificável e 74,69-88,16 mg kg–1 de clorofila.

  12. International Recovery Plan : Whooping Crane (Grus americana) : Third Revision

    US Fish and Wildlife Service, Department of the Interior — In the United States, the whooping crane (Grus americana) was listed as threatened with extinction in 1967 and endangered in 1970 – both listings were...

  13. Final Critical Habitat for the Whooping crane (Grus americana)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where the final critical habitat for Whooping crane (Grus americana) occur based on the description provided in the...

  14. A New Pregnane Glycoside from Fermented Leaves of Agave americana

    2002-01-01

    A new minor pregnane glycoside was isolated from the fermented leaves of Agave americana. Its structure was elucidated as (20S)-5α-pregnane-3β, 20-diol 20-O-β-D-glucopyrano- side (1) by spectral methods.

  15. Psicologia Latino-Americana: desafios e possibilidades

    Camilla Veras Pessoa da Silva

    Full Text Available A comemoração dos 50 anos da regulamentação da profissão da Psicologia no Brasil sinaliza o recente e o contínuo esforço das profissionais psicólogas e psicólogos em contextualizar o saber psicológico com a realidade e as condições de vida da população latino-americana. Atualmente, a Psicologia passa por um processo de reformulação crítica que considera as peculiaridades históricas, culturais, políticas, econômicas e sociais na constituição subjetiva dos sujeitos. Dessa forma, propõe o alinhamento epistemológico desse saber com os demais países da América Latina devido às vivências similares de colonização ibérico-católica, de modernização tardia e de exploração dos recursos naturais e humanos do continente. Contudo, a construção de uma psicologia latino-americana enfrenta desafios, a exemplo das diferenças entre os países, da multiplicidade cultural e dos interesses geopolíticos das nações. O objetivo do presente estudo foi compreender o processo de formação e de construção epistemológica da Psicologia histórico-cultural buscando, na literatura analisada, as principais possibilidades e desafios da integração da Psicologia com os demais países latino-americanos. De qualquer forma, observa-se que, ao se dedicar ao atendimento das demandas populacionais e à implicação no processo de transformação social, são oferecidas à Psicologia diversas possibilidades de atuação.

  16. 古波斯萨珊王朝珍贵文物《鎏金银壶》的仿制%A Model After Precious Cultural Relic《Silver pot gilt with gold》from a Certain Ancient Persia Dynasty

    刘军荣; 胡春良

    2013-01-01

    对古波斯萨珊王朝的手工艺杰作《鎏金银壶》的仿制进行简要介绍,特别是浇冒口设计、制壳、合金冶炼、后加工及表面处理的工艺控制与操作。%It has been briefly introduced a model after a handcraft masterpiece《Silver pot gilt with gold》from a certain ancient Persia dynasty, especially with technology control and operation as pouring gate and riser design, shell forming, alloy melting, post-processing and superficial treatment.

  17. A Rússia Americana The American Russia

    João Marcelo Ehlert Maia

    2005-08-01

    Full Text Available Este artigo procura analisar interpretações do Brasil assentadas na idéia de que seríamos uma "Rússia Americana". Para tanto, analiso as contribuições de Gilberto Freyre, Otávio Velho e Vicente Licínio Cardoso, e apresento brevemente o caso russo, a partir das obras clássicas de Franco Venturi e Isaiah Berlin. Argumento ao longo do texto que essa percepção de que o Brasil teria uma geografia social "russa" implica a produção de um americanismo específico, que encontra sua maior expressão no pensamento de Vicente Licínio Cardoso e em suas idéias que conjugam fordismo e arielismo.This article seeks to analyse Brazil's interpretations founded on the idea that we would be an "American Russia". in order to do so, I analyse the contributions of Gilberto Freyre, Otávio Velho and Vicente Licínio Cardoso, and I briefly present the russian case, using the works of Franco Venturi and Isaiah Berlin. I suggest that this perception implies the production of an especific type of americanism, wich finds its greater expression on the unusual combination between fordism and arielism made by Vicente Licínio.

  18. Assimilate partitioning in avocado, Persea americana

    Finazzo, S.; Davenport, T.L.

    1986-04-01

    Assimilate partitioning is being studied in avocado, Persea americana cv. Millborrow in relation to fruit set. Single leaves on girdled branches of 10 year old trees were radiolabeled for 1 hr with 13..mu..Ci of /sup 14/CO/sub 2/. The source leaves were sampled during the experiment to measure translocation rates. At harvest the sink tissues were dissected and the incorporated radioactivity was measured. The translocation of /sup 14/C-labelled compounds to other leaves was minimal. Incorporation of label into fruitlets varied with the tissue and the stage of development. Sink (fruitlets) nearest to the labelled leaf and sharing the same phyllotaxy incorporated the most /sup 14/C. Source leaves for single non-abscising fruitlets retained 3X more /sup 14/C-labelled compounds than did source leaves for 2 or more fruitlets at 31 hrs. post-labelling. Export of label decreased appreciably when fruitlets abscised. If fruitlets abscised within 4 days of labeling then the translocation pattern was similar to the pattern for single fruitlets. If the fruitlet abscised later, the translocation pattern was intermediate between the single and double fruitlet pattern.

  19. Propolis and vegetable wax in the conservation of Hass avocadoPrópolis e cera vegetal na conservação de abacate Hass

    Ricardo de Oliveira Orsi

    2012-08-01

    Full Text Available The aim of this work was to evaluate the quality of Hass avocado submitted to application of propolis and vegetable wax. The selected fruits were submitted to the treatments (1 without wax and without propolis, (2 with vegetable wax, (3 propolis alcoholic extract 100%, (4 wax and etanol 70%, (5 propolis alcoholic extract 2% and wax, (6 propolis alcoholic extract 4% and wax, (7 propolis alcoholic extract 6% and wax, (8 propolis alcoholic extract 8% and wax, and (9 propolis alcoholic extract 10% and wax. The fruits were maintained under refrigeration (10 ± 1 ºC and 90 ± 5% relative humidity, being appraised every three days for 15 days and more 3 days of commercialization simulation at room temperature (23± 1 ºC and 18% relative humidity.The evaluation parameters were the weight loss, respiratory activity, pH, firmness, tritable acidity (AT, soluble solids (SS, and ratio. The results of weigth loss, respiratory activity and firmness were the most significant to show the difference among the treatments. ‘Hass’ avocado fruits submitted the application of propolis associated the vegetable wax present smaller weight loss, CO2 production and firmness in relation to the control fruits. The fruits of the treatment with propolis alcoholic extract 2% and wax presented the most promising results. Objetivou-se neste trabalho avaliar a aplicação de própolis e cera vegetal na qualidade pós-colheita do abacate ‘Hass’. Os frutos selecionados foram submetidos aos tratamentos (1 sem cera e sem própolis, (2 com cera vegetal, (3 extrato alcoólico de própolis 100%, (4 cera e etanol 70%, (5 extrato alcoólico de própolis 2% e cera, (6 extrato alcoólico de própolis 4% e cera, (7 extrato alcoólico de própolis 6% e cera, (8 extrato alcoólico de própolis 8% e cera e (9 extrato alcoólico de própolis 10% e cera. Os frutos foram mantidos sob refrigeração (10 ± 1 ºC e 90 ± 5% umidade relativa, sendo avaliados a cada três dias durante 15 dias

  20. Analysis of sesquiterpene distributions in leaves, branches, and trunks of avocado (Persea americana Mill.)

    Avocado is a commercially valuable fruit crop cultivated in tropical and subtropical climates throughout the world. Taxonomists recognize three horticultural races of avocado, consisting of Mexican (Persea americana var. drymifolia), Guatemalan (P. americana var. guatemalensis), and West Indian (P. ...

  1. Aspectos quimiotaxonómicos en Myristicaceae Americanas Aspectos quimiotaxonómicos en Myristicaceae Americanas

    Aguirre Gálviz Luis Enrique

    1990-06-01

    Full Text Available

    Se hace un análisis del patrón de distribución de agliconas de flavonoides y ácidos hidroxiaromáticos en 187 muestras de 33 especies de Myristicaceae americanas y, junto con la información morfo1ógica disponible, se presenta un enfoque biosistemático para el conocimiento taxonómico de la familia.

    A survey of the distribution of flavonoid aglycones and related bydroxy-aromatic acids in 187 samples of 33 species of American Myristicaceae is carried out. This survey, together with the available morfological information, provides a biosynthetic approach to the taxonomy of the family.

  2. Characterization and development mechanism of Apios americana tuber starch

    Apios americana is a wild legume-bearing plant with edible tubers. Domestication of Apios is in progress because of the superior nutritional value and health benefits of the tuber. Objectives of this study were to: 1) characterize physicochemical properties of Apios-tuber starch; and 2) understand d...

  3. Teaching German-Americana with Assistance from the Web.

    Shea, Robert J.; Hoyt, Giles R.

    1998-01-01

    Argues that the World Wide Web can assist in teaching about German-Americana in German-language instruction, and discusses some basic Web page uses to find and organize literary texts, syllabi, course outlines, images and realia, and information about people, organizations, events, and places. Some of the most useful German-American resources are…

  4. Hypoglycemic and hypocholesterolemic potential of Persea americana leaf extracts.

    Brai, Bartholomew I C; Odetola, A A; Agomo, P U

    2007-06-01

    The effect of aqueous and methanolic leaf extracts of Persea americana on plasma glucose, total cholesterol, low-density lipoprotein cholesterol (LDL-CHOL), and high-density lipoprotein cholesterol (HDL-CHOL) in rats was investigated. Albino rats were fed a diet containing 20% groundnut oil, 0.5% cholesterol, and 0.25% cholic acid to induce hypercholesterolemia. They were then treated daily with aqueous or methanolic extract of P. americana leaf (10 mg/kg of body weight) for 8 weeks. There were no significant (P > .05) differences in the overall body weight gain of the hypercholesterolemic rats compared to normal control. Liver to body weight ratio, plasma glucose, total cholesterol (T-CHOL), and LDL-CHOL levels were significantly (P americana induced reductions in plasma glucose (16% and 11%,respectively), T-CHOL (8% and 5%, respectively), and LDL-CHOL (19% and 20%, respectively) in the treated rats compared to the hypercholesterolemic controls. Also, plasma HDL-CHOL concentrations increased by 85% and 68%, respectively, in the aqueous and methanolic extract-treated rats compared to the hypercholesterolemic controls. These results suggest that aqueous and methanolic leaf extracts of P. americana lower plasma glucose and influence lipid metabolism in hypercholesterolemic rats with consequent lowering of T-CHOL and LDL-CHOL and a restoration of HDL-CHOL levels. This could represent a protective mechanism against the development of atherosclerosis.

  5. Parasites of Periplaneta americana linnaeus "domestic cockroach" from Ica.

    Mary Fernádez B.

    2014-06-01

    Full Text Available 244 specimens of Periplaneta americana Linnaeus from 13 localities of Ica were studied. Nematodes and protozoa were identified. They are Lophomonas blattarum, Leptomonas sp., Leidynema appendiculatum and Hammerschmidtiella diesingi. Also, parasites of man were found, three of which are pathogenic: Giardia lamblia, Blastocystis hominis and Cryptosporidium sp.

  6. Three new hecogenin glycosides from fermented leaves of Agave americana.

    Jin, Jian-Ming; Liu, Xi-Kui; Yang, Chong-Ren

    2003-06-01

    Eight steroidal compounds, including three new hecogenin glycosides, agamenosides D-F, were isolated from the fermented leaves of Agave americana. The structures of the new steroidal saponins were elucidated by spectroscopic data and chemical methods. The activity of the isolated compounds on deformations of mycelia germinated from conidia of Pyricularia oaryzae P-2b was evaluated.

  7. Tratamento da leishmaniose tegumentar americana pelo niridazol

    Miroslau Constante Baranski

    1970-08-01

    Full Text Available Foi empregue o niridazol um derivado do nitrotiazol, em doze pacientes com leishmaniose tegumentar americana. Em todos êles, o diagnóstico clínico da doença foi confirmado pela biópsia das lesões e em oito dêles também pela positividade da intradetermorreação de Montenegro. Oito pacientes tinham lesões mucosas metastáticas em atividade e lesões cutâneas iniciais cicatrizadas há tempo mais ou menos longo. Dois apresentavam concomitância de lesões cutâneas e mucosas em atividade, e dois tinham lesões cutâneas exclusivas. A duração da doença variou de 2 meses a 32 anos. A posologia diária do niridazol foi uniformememente de 25 mg/kg de pêso corpóreo. O medicamento foi administrado por via oral, em duas tomadas diárias, sempre com o doente internado em hospital. Quando a tolerância o permitia, o paciente recebia cinco séries de tratamento de 10 dias de duração cada, intercaladas por períodos áe suspensão da droga de 10 dias entre uma série e outra. Isso foi possível em 10 dos 12 pacientes. O tratamento foi bem tolerado em 5 doentes e de tolerância regular em 4. Mal tolerado em um paciente pela ocorrência de alucinações e excitação mental e interrompido pela péssima tolerância em dois doentes devido ao aparecimento de convulsões generalizadas com perda da consciência. O tratamento acompanhou-se de grande incidência, de efeitos colaterais. A ocorrência de para-efeitos mais intensos não foi devida às más condições hepáticas. Provas de função hepática. hemogramas, estudos bioquímicos do sangue e exames de urina, realizados antes, durante e após o tratamento, não revelaram alterações significativas. Biópsias hepáticas por punção com agulha em dez doentes, prévias e posteriores ao tratamento, não detectaram lesões hepáticas que pudessem ser atribuídas à medicação. O seguimento dos doentes prolongou-se pelo prazo de dois a 36 meses apos o tratamento. Alterações eletrocardiogr

  8. Enfermedad de Chagas o Tripanosomiasis Americana.

    Felipe Guhl

    2000-08-01

    Full Text Available

    Situación Actual de Colombia.

    La enfermedad de Chagas o tripanosomiasis americana es una enfermedad parasitaria crónica causada por un protozoario flagelado el Trypanosoma cruzi, descrito por primera vez por Carlos Chagas, médico brasileño, a comienzos de este siglo y en su honor se denominó la enfermedad que lleva su nombre.

    Este parásito normalmente se transmite al ser humano a través de insectos triatomíneos estrictamente hematófagos de la familia Reduviidae, en el momento en que perforan la piel para succionar la sangre que los alimenta.

    Sin embargo, no se inocula directamente por intermedio de las estructuras bucales del insecto en el momento de la picadura como en el caso de las tripanosomiasis africanas, si no que se deposita pasivamente en la piel a través de las heces del insecto, penetrando en el cuerpo por la herida que causa la picadura u otras abrasiones de la piel o de la mucosa. El T. cruzi, también puede transmitirse por infección congénita, por transfusión de sangre contaminada o por el transplante de órganos contaminados. El ciclo vital del parásito es largo y complejo y su desarrollo tiene varias etapas, tanto en el vector triatomineo como en el huésped vertebrado .

    La Enfermedad de Chagas constituye una amenaza permanente para casi la cuarta parte de toda la población de América Latina. Si bien la enfermedad se encuentra presente en toda América Central y del Sur, sus manifestaciones y características epidemiológicas son altamente variables entre una y otra zona endémica. Existe una gran diversidad en las tasas de prevalencia, formas de transmisión, características parasitarias, patología clínica, vectores y reservorios.

    Más que cualquier otra enfermedad parasitaria, la enfermedad de Chagas se relaciona con el desarrollo económico y social de la población: los insectos triatomineos y las enfermedades que ellos transmiten existirán mientras en

  9. Identificação de espécies de Fusicoccum causadoras de podridão em frutos de abacate

    Ana Carolina Firmino

    2016-03-01

    Full Text Available ABSTRACT The Fusicoccum genus of fungi are known to cause stem-end rot in various fruit plants, such as mango, guava, peach and avocado. Several species of this fungus are reported attacking avocado (Persea americana in several countries. Based on this information, the present study aimed to identify species of Fusicoccum associated with rot in avocado fruits in the State of São Paulo. Samples were collected (fruits with rot symptoms from regions of Bauru, Bernadino de Campos and Piraju. All isolates obtained had its pathogenicity confirmed by inoculation of healthy avocado fruits. After confirming its pathogenicity, these isolates had their DNA extracted and the ITS-5.8S rDNA region was amplified. After editing, these sequences were used to search for similar sequences in the NCBI. Eleven samples were identified as Neofusicoccum parvumand others were identified as Botryosphaeria dothidea(F. aesculi. Both species were found in all regions of collection.

  10. Production of plant virus inhibitor by Phytolacca americana suspension culture.

    Misawa, M; Hayashi, M; Tanaka, H

    1975-09-01

    The inhibitory activity of tobacco mosaic virus (TMV) infection was assayed with the extracts of various callus tissues derived from the intact plants. Phytolacca americana callus was selected as a producer of the virus inhibitor and its cultural conditions in suspension were examined for cell growth and the inhibitor production. A modified liquid medium containing twofold concentrations of all components in that of Murashige and Skoog plus2,4-D (1.0 mg/liter) and sucrose (6%), but without any vitamins and glycine was chosen for production of higher levels of the inhibitor. TMV infections in tobacco, bean, and tomato plants were markedly inhibited by the introduction of the disrupted whole broth of suspension cultured P. americana.

  11. Aspectos quimiotaxonómicos en myristicaceae americanas

    Aguirre Gálviz, Luis Enrique

    2011-01-01

    Se hace un análisis del patrón de distribución de agliconas de flavonoides y ácidos hidroxiaromáticos en 187 muestras de 33 especies de Myristicaceae americanas y, junto con la información morfo1ógica disponible, se presenta un enfoque biosistemático para el conocimiento taxonómico de la familia.

  12. A new steroidal saponin from the leaves of Agave americana.

    Yokosuka, A; Mimaki, Y; Kuroda, M; Sashida, Y

    2000-05-01

    A new bisdesmosidic spirostanol saponin, along with three known saponins, were isolated from Agave americana (Agavaceae). The structure of the new saponin was elucidated as (25R)-3 beta,6 alpha-dihydroxy-5 alpha-spirostan-12-one 3,6-di-O-beta-D-glucopyranoside. Among the isolated saponins, hecogenin tetraglycoside showed cytotoxic activity against HL-60 human promyelocytic leukemia cells with an IC50 value of 4.3 micrograms/mL.

  13. Bioactive compounds and antioxidant potential fruit of Ximenia americana L.

    Almeida, Maria Lucilania Bezerra; Freitas, Wallace Edelky de Souza; de Morais, Patrícia Lígia Dantas; Sarmento, José Dárcio Abrantes; Alves, Ricardo Elesbão

    2016-02-01

    The caatinga ecoregion in northeast Brazil presents a wide variety in plant species. However, the potential of these species as a source of energy, carbohydrates, vitamins, minerals and bioactive properties beneficial to health is still unknown. Among these species we can find the wild plum (Ximenia americana). Due to its various phytotherapeutic properties and absence of studies on the chemical composition of the fruit this article aimed to evaluate the bioactive compounds and antioxidant potential of the X. americana in different stages of maturation. The fruits of X. americana showed considerable amounts of bioactive compounds, as well as antioxidant activity and antioxidant enzymes. The fruits at green maturity stage showed higher content of yellow flavonoids (22.07 mg/100g), anthocyanins (1.92 mg/100 g), polyphenols (3051.62 mg/100 g), starch (4.22%), antioxidant activity (489.40 g fruit/g DPPH and 198.77 μmol Trolox/g) and activity of antioxidant enzymes; the antioxidant activity allocated to the fruit was shown to be related to the contents of extractable polyphenols, yellow flavonoids, total anthocyanins and antioxidant enzymes.

  14. Desempenho da tangerineira 'Span Americana' em diferentes porta-enxertos

    Simone Rodrigues da Silva

    2013-12-01

    Full Text Available Este trabalho objetivou avaliar o crescimento vegetativo, a produção e a qualidade de frutos da tangerina 'Span Americana' em diferentes porta-enxertos, nas condições edafoclimáticas de Bebedouro-SP. O plantio foi realizado em junho de 2003, em espaçamento de 6,0 m x 3,0 m, sendo utilizada irrigação por gotejamento a partir de 2006. Os porta-enxertos avaliados foram: citranges [Citrus sinensis (L. Osbeck × Poncirus trifoliata L. Raf] 'Carrizo' e 'Troyer', tetraploides; trifoliatas (P. trifoliata 'Davis A' e 'Flying Dragon'; limão Volkameriano Catania 2 (C. volkameriana Tenn. et Pasq., HRS 849 [(C. aurantium L. cv. 'Smooth Flat Seville' x P. trifoliata cv. 'Argentina'], tangelo 'Orlando' (C. reticulata Blanco × C. paradisi Macf. e limão 'Cravo' (C. limonia Osbeck. Foram avaliadas a produção acumulada, a eficiência produtiva e a precocidade de entrada em produção, no período de 2007 a 2009. Avaliaram-se, também, as dimensões das plantas e a taxa média de crescimento das plantas no período de 2005 a 2008, além da qualidade dos frutos em 2006 e 2007. Em pomares irrigados de tangerineira 'Span Americana', os porta-enxertos trifoliata 'Davis A' e HRS 849 apresentam desempenho horticultural satisfatório. Para plantio em alta densidade, a melhor performance da tangerineira 'Span Americana' é obtida com a utilização dos porta-enxertos trifoliata 'Flying Dragon' e citranges 'Troyer' e 'Carrizo'.

  15. La esclavitud americana y las Partidas de Alfonso X

    Lucena Salmoral, Manuel

    1995-01-01

    El autor expone de manera sorprendente la relación entre la esclavitud americana en 1808, en los albores de la Independencia y su coincidencia con un conjunto de leyes como fueron las Partidas de Alfonso X, hechas siete siglos antes. Dentro del marco de las Partidas, que fueron el único Código Negro que la América española tuvo en su vida colonial, se centra en la servidumbre del hombre como mal antinatural y la manumisión de los siervos. The author exposes in an amazing way the relationsh...

  16. Componentes volátiles de mamey (mammea americana L.)

    Alicia Lucía Morales; William Arguello; Gustavo García; Iván Herrera

    2010-01-01

    Los componentes volátiles del aroma de mamey (Mammea americana L), fueron extraídos utilizando el método de destilación por arrastre con vapor-extracción simultánea con solvente orgánico. El extracto fue prefraccionado por cromatografía en columna en silica gel con gradiente discontinuo Pentano: Éter etílico para obtener tres fracciones que fueron analizadas por CGAR y CGAR-EM. Se detectaron 34 compuestos, de los cuales fueron identificados 22, siendo los componentes mayoritarios: Furfural (7...

  17. Componentes volátiles de mamey (mammea americana l.)

    Morales, Alicia Lucía; Arguello, William; García, Gustavo; Herrera, Iván

    2010-01-01

    Los componentes volátiles del aroma de mamey (Mammea americana L), fueron extraídos utilizando el método de destilación por arrastre con vapor-extracción simultánea con solvente orgánico. El extracto fue prefraccionado por cromatografía en columna en silica gel con gradiente discontinuo Pentano: Éter etílico para obtener tres fracciones que fueron analizadas por CGAR y CGAR-EM. Se detectaron 34 compuestos, de los cuales fueron identificados 22, siendo los componentes mayoritarios: Furfural (7...

  18. Heterobilharzia americana Infection and Glomerulonephritis in a Dog.

    Ruth, Jeffrey

    2010-01-01

    Schistosomiasis is an uncommonly reported disease that usually causes weight loss, anemia, and gastrointestinal signs. A 6-year-old, neutered male dog developed membranoproliferative glomerulonephritis concurrent with infection with the trematode parasite Heterobilharzia americana. At presentation, the dog had proteinuria, hypoalbuminemia, hyperglobulinemia, and anemia. Diagnosis was based upon the histopathological appearance of the kidney. Clinical signs, biochemical and hematological abnormalities, and proteinuria resolved following treatment with fenbendazole and praziquantel. Fecal examination by saline sedimentation, miracidia hatching, or Heterobilharzia polymerase chain reaction assay may be indicated when examining a dog that is presented with unexplained glomerulonephritis and is from an endemic area.

  19. Leishmaniose tegumentar americana associada à AIDS: relato de quatro casos

    Sampaio Raimunda Nonata Ribeiro; Salaro Cristina Paula; Resende Paula; Paula Carmen Déa Ribeiro de

    2002-01-01

    A co-infecção leishmaniose tegumentar americana e AIDS é de relato recente na literatura, observando-se diversidade quanto ao comportamento clínico e imunológico destes pacientes. Relatamos quatro casos acompanhados no Hospital Universitário de Brasília, com diagnóstico de infecção por parasitas do gênero Leishmania e pelo vírus da imunodeficiência humana, ilustrando diferentes apresentações clínicas, evoluções e respostas terapêuticas.

  20. Isocoumarins from American cockroach (Periplaneta americana) and their cytotoxic activities.

    Luo, Shi-Lin; Huang, Xiao-Jun; Wang, Ying; Jiang, Ren-Wang; Wang, Lei; Bai, Liang-Liang; Peng, Qun-Long; Song, Cai-Lu; Zhang, Dong-Mei; Ye, Wen-Cai

    2014-06-01

    Four new isocoumarins (1-4), along with three known ones (5-7), were isolated from the 70% ethanol extract of the whole body of the traditional Chinese insect medicine, American cockroach (Periplaneta americana). The structures with absolute configurations of new compounds were elucidated by extensive spectroscopic methods in combination with X-ray diffraction experiment and CD analyses. Compounds 3-5 showed significant cytotoxic activities in HepG2 and MCF-7 cells with IC50 values in the ranges 6.41-23.91 μM and 6.67-39.07 μM, respectively.

  1. Nongastrointestinal helminths in marten (Martes americana) from Ontario, Canada.

    Seville, R S; Addison, E M

    1995-10-01

    Six species of nongastrointestinal nematodes were recovered from 405 marten, (Martes americana), examined from six areas in Ontario, Canada in 1992 to 1993. Three species (Crenosoma petrowi, Eucoleus aerophilus, Filaroides martis) were found in the respiratory tract, one in the urinary bladder (Pearsonema plica), one in the kidney (Dioctophyme renale), and one in the musculature (Trichinella sp. larvae). This is the first report of F. martis and P. plica from this host. In addition a specimen of Dracunculus insignis collected from a marten pelt was received. Based on our results, martens are primary definitive hosts for few nongastrointestinal nematodes. Animals in more southern areas had greater species richness than those from higher latitudes.

  2. Purpuric irritant contact dermatitis induced by Agave americana.

    Cherpelis, B S; Fenske, N A

    2000-10-01

    The sap of Agave americana, a popular ornamental plant, may cause irritant contact dermatitis. This rare eruption is typically vesiculopapular; however, a new purpuric variant with evidence of leukocytoclastic vasculitis has recently been reported. We report an additional case of a purpuric eruption associated with severe constitutional symptoms further supporting a possible vasculitic component. Both cases resulted from direct exposure to sap propelled by a chainsaw. We speculate that oxalic acid crystals, which are recognized systemic toxins, are embedded in the skin with resulting oxalism, which may result in vascular damage.

  3. The phytochemical and pharmacological profile of Persea americana Mill

    Mohammad Yasir

    2010-01-01

    Full Text Available Over the past decades, herbal medicine has become a thing of global significance with medicinal and economic implications. Wide spread use of herbs throughout the globe has raised serious concerns over its quality, safety, and efficacy. Thus, exact scientific assessment has become a precondition for acceptance of herbal health claims. Persea americana Mill. (avocado is a tree, native to central America, cultivated in tropical and subtropical climates around the world, belonging to the family Lauraceae, is widely used in Ayurveda and evidence-based phototherapy. There are 3 principal races or groups of avocado: Mexican, Guatemalan, and West Indian named for the areas where they were originally cultivated. The plant is used in traditional medicine for the treatment of various ailments, such as monorrhagia, hypertension, stomach ache, bronchitis, diarrhea, and diabetes. Peptone, b-galactoside, glycosylated abscisic acid, alkaloids, cellulose, polygalacto urease, polyuronoids, cytochrome P-450, and volatile oils are reported to be present in this plant. Biotechnologic approaches show that modified MS medium supplemented with 1.0 mg benzyladenine/L, 0-1mg Indole Butyric Acid/L, 0.1 mg Gibberalic Acid 3/L was optimum for adventitious shoot development. In the present review, an effort has been made to study the different aspects of P. americana Mill.

  4. The phytochemical and pharmacological profile of Persea americana Mill.

    Yasir, Mohammad; Das, Sattwik; Kharya, M D

    2010-01-01

    Over the past decades, herbal medicine has become a thing of global significance with medicinal and economic implications. Wide spread use of herbs throughout the globe has raised serious concerns over its quality, safety, and efficacy. Thus, exact scientific assessment has become a precondition for acceptance of herbal health claims. Persea americana Mill. (avocado) is a tree, native to central America, cultivated in tropical and subtropical climates around the world, belonging to the family Lauraceae, is widely used in Ayurveda and evidence-based phototherapy. There are 3 principal races or groups of avocado: Mexican, Guatemalan, and West Indian named for the areas where they were originally cultivated. The plant is used in traditional medicine for the treatment of various ailments, such as monorrhagia, hypertension, stomach ache, bronchitis, diarrhea, and diabetes. Peptone, b-galactoside, glycosylated abscisic acid, alkaloids, cellulose, polygalacto urease, polyuronoids, cytochrome P-450, and volatile oils are reported to be present in this plant. Biotechnologic approaches show that modified MS medium supplemented with 1.0 mg benzyladenine/L, 0-1mg Indole Butyric Acid/L, 0.1 mg Gibberalic Acid 3/L was optimum for adventitious shoot development. In the present review, an effort has been made to study the different aspects of P. americana Mill.

  5. 'Gloriosa': cultivar de alface americana tropicalizada 'Gloriosa': crisphead tropicalized cultivar

    Fernando C. Sala

    2008-09-01

    Full Text Available 'Gloriosa' é uma cultivar de alface (Lactuca sativa L. americana tropicalizada e resistente a Thielaviopsis basicola. Foi selecionada a partir de variantes da cultivar Lucy Brown, pelo método genealógico. Progênies S1 a S6 foram selecionadas para resistência a T. basicola, características hortícolas, tolerância ao pendoamento precoce e adaptação ao cultivo de verão. Apresenta ciclo precoce de 40 a 50 dias pós-transplante, pendoamento lento, cabeças grandes e três camadas de folhas externas. Possui tolerância a Xanthomonas campestris pv. vitians e suporta o cultivo nos períodos de elevadas temperaturas e pluviosidade. Trata-se da primeira cultivar de alface americana tropicalizada no Brasil.'Gloriosa' is a tropicalized crisphead lettuce (Lactuca sativa L. resistant to Thielaviopsis basicola. It was derived by selection from cv. Lucy Brown variants by pedigree method. Progenies S1 to S6 were selected for T. basicola resistance, desirable horticulture traits, slowbolting and adaptation for Brazilian summer crop. It presents a precocious cycle within 40 to 50 days of being planted, slowbolting, large head and three layers of frame levels. It is tolerant to Xanthomonas campestris pv. vitians and is able to be grown under high temperature and rainy summer in southern of Brazil. It is the first crisphead lettuce type developed for tropical regions of Brazil.

  6. Heterobilharzia americana infection and congestive heart failure in a llama (Lama glama).

    Corapi, W V; Eden, K B; Edwards, J F; Snowden, K F

    2015-05-01

    The schistosome Heterobilharzia americana infects several mammalian species in the southeastern United States, including horses, but infections have not been reported in camelids. This is a report of H. americana infection in a 6-year-old llama with extensive cardiac pathology and congestive heart failure. Parasite-induced granulomas were widely disseminated and included overwhelming involvement of the lungs and liver. Microscopic lesions in the heart included myofiber degeneration and necrosis, with extensive replacement fibrosis. Polymerase chain reaction amplification and sequencing confirmed the presence of H. americana in the lungs.

  7. Efficacy of entomopathogenic hypocrealean fungi against Periplaneta americana.

    Hubner-Campos, Rayssa Fátima; Leles, Renan Nunes; Rodrigues, Juscelino; Luz, Christian

    2013-12-01

    The American cockroach Periplaneta americana, one of the worlds' most important urban insect pests was tested with entomopathogenic fungi. Most promising Metarhizium anisopliae, Metarhizium robertsii and Beauveria bassiana killed nymphs (≥ 81.7% mortality, 25 days after treatment), and these fungi developed on all dead insects. Other fungi tested were less virulent (Metarhizium frigidum and Purpureocillium lilacinum) or avirulent (Isaria cateniobliqua, Isaria farinosa, Simplicillium lanosoniveum, Sporothrix insectorum and Tolypocladium cylindrosporum). Intrageneric and intraspecific variability of fungal activity was detected. Adults were highly susceptible, and oothecae proved to be more resistant than nymphs and adults to infection with M. anisopliae IP 46. Findings of the study underscore the potential of fungi as biocontrol agents against this pest.

  8. Componentes volátiles de mamey (mammea americana L.

    Alicia Lucía Morales

    2010-07-01

    Full Text Available Los componentes volátiles del aroma de mamey (Mammea americana L, fueron extraídos utilizando el método de destilación por arrastre con vapor-extracción simultánea con solvente orgánico. El extracto fue prefraccionado por cromatografía en columna en silica gel con gradiente discontinuo Pentano: Éter etílico para obtener tres fracciones que fueron analizadas por CGAR y CGAR-EM. Se detectaron 34 compuestos, de los cuales fueron identificados 22, siendo los componentes mayoritarios: Furfural (7281 ^ig/kg y E-Famesol (2145 ng/kg

  9. Pretreatment of Agave americana stalk for enzymatic saccharification.

    Yang, Qiang; Pan, Xuejun

    2012-12-01

    Agave americana is one of commonly grown agave species but currently less valuable because its large flower stalk cannot be used for producing alcoholic beverage. In the present study, the stalk was pretreated with dilute acid (DA), sulfite (SPORL), and sodium hydroxide (NaOH) to preliminarily assess its potential as feedstock for bioethanol production. The changes of cell wall components during the pretreatments, enzymatic digestibility of the pretreated stalks, and the adsorption of cellulases on the substrates were investigated. Results indicated that the pretreatments significantly improved the enzymatic digestibility of the agave stalk. SPORL pretreatment gave higher substrate and sugar yields, while NaOH pretreated stalk had better digestibility under the investigated conditions. The better hydrolysability of NaOH-pretreated stalk was attributed to low lignin and hemicellulose content and high affinity to cellulases.

  10. Two New Steroidal Glycosides from Fermented Leaves of Agave americana

    JianMingJIN; XiKuiLIU; 等

    2002-01-01

    Two new spirostanol glycosides named agamenoside A and B, ere isolated from the fermented leaves of Agave americana. Their structures were elucidated as (23S,25R)-5α-spirostan-3β,6α,23-triol 3-O-α-L-rhamnopyranosyl-(1→3)-β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(1) and (25R)-5α-spiro-stan-3β,6α-diol 3-O-β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyra-nosyl-(1→4)-β-D-galactopyranoside(2) by a combination of chemical and spectral methods.

  11. Two New Steroidal Glycosides from Fermented Leaves of Agave americana

    2002-01-01

    Two new spirostanol glycosides named agamenoside A and B, were isolated from the fcrmcnted leaves of Agave americana. Their structures were elucidated as (23S, 25R)-5α-spirostan-3β, 6α, 23-triol 3-O-α-L-rhamnopyranosyl-(1→3)- β-D-glucopyranosyl-(l→2)-[β-D-xylopyranosyl-( 1 →3)]-β-D-glucopyranosyl-( l →4)-β-D-galactopyranoside (1) and (25R)-5α-spiro stan-3β, 6α-diol 3-O-β-D-glucopyranosyl-(l→2)-[ β-D-xylopyranosyl-(l→3)]- β-D-glucopyra nosyl-(1→4)- β-D-galactopyranoside (2) by a combination of chemical and spectral methods.

  12. Properties of peroxisomal and mitochondrial citrate synthase from Agave americana.

    Segovia, J L; Zafra, M F; Alejandre, M J; García-Peregrín, E

    1982-09-01

    Adenine nucleotides were tested as effectors of peroxisomal and mitochondrial citrate synthase from Agave americana leaves in the presence of different concentrations of acetyl-CoA and oxalacetate substrates. ATP inhibited both enzyme activities but with a different inhibition profile. 1.0-7.5 mM ADP did not inhibit the peroxisomal citrate synthase in the presence of high substrate concentrations, while the mitochondrial enzyme was strongly inhibited by 1.0 mM ADP in the same conditions. Likewise, a different pattern was obtained with AMP on both peroxisomal and mitochondrial activities. The rate of citrate formation as function of acetyl-CoA and oxalacetate concentration was also studied in both fractions. Maximal velocity was highest in the peroxisomal fraction, whether acetyl-CoA or oxalacetate were the variable substrates. These differences indicate that peroxisomal and mitochondrial citrate synthases seem to be two different isoenzymes.

  13. Swimming black-crowned night-herons (Nycticorax nycticorax) Kleptoparasitize American coots (Fulica americana)

    Graves, Gary R.

    2015-01-01

    I observed black-crowned night-herons (Nycticorax nycticorax) swimming and kleptoparasitizing American coots (Fulica americana) at an artificial lake in Pinal County, Arizona. This appears to be the first record of interspecific kleptoparasitism by a swimming ardeid.......I observed black-crowned night-herons (Nycticorax nycticorax) swimming and kleptoparasitizing American coots (Fulica americana) at an artificial lake in Pinal County, Arizona. This appears to be the first record of interspecific kleptoparasitism by a swimming ardeid....

  14. A Case of Ventilator-Associated Pneumonia Caused by Ewingella americana : First Report from Turkey

    Kenan Ecemiş

    2014-09-01

    Full Text Available Ewingella americana is a very rare cause of serious infections, especially in immunocompromised patients. We report a case of ventilator-associated pneumonia (VAP in an intensive care unit patient, who was treated successfully with ceftriaxone. To the best of our knowledge, this is the first case of VAP due to Ewingella americana in the English literature.J Microbiol Infect Dis 2014; 4(3: 118-120

  15. Modelos de masculinidad en la literatura chino-americana contemporánea

    Soria Somoza, Carolina

    2016-01-01

    [Resumen] Modelos de masculinidad en la literatura chino-americana contemporánea Esta tesis doctoral estudia los modelos de masculinidad presentes en la literatura chino-americana contemporánea. Para ello, analizo las obras de autores tan representativos como Frank Chin, Louis Chu, David Henry Hwang, Gish Jen, Ha Jin y Maxine Hong Kingston. Mi principal objetivo es examinar las diferencias entre aquellos modelos que resulten obsoletos y estereotípicos de aquellos que puedan ...

  16. The avocado fruit borer, Stenoma catenifer (wals. (Lepidoptera: elachistidae: egg and damage distribution and parasitism A broca-do-abacate, Stenoma catenifer (wals. (Lepidoptera: elachistidae: distribuição de ovos e de danos e parasitismo

    Celso Luiz Hohmann

    2003-12-01

    Full Text Available The avocado fruit borer, Stenoma catenifer (Wals. has been a limiting factor in growing avocados over the last years in many Brazilian states. This is a result of the lack of safe and feasible management practices to minimize the fruit borer damage. The aim of this study was to obtain information on the pest biology and ecology as well as on the role of natural enemies to define strategies to control the pest. Samples were taken biweekly and consisted of 20 fruits collected randomly (10 from the upper half and 10 from the lower half of the plant in ten plants, cv. Margarida, in a commercial avocado grove in Arapongas and Cambé regions, PR, from October/2001 to September/2002. Laboratory determinations of the percentage of damaged fruit per plant region, location and number of bored fruit sites, and the number and location of the fruit borer eggs, including parasitized ones, were performed. The results showed that S. catenifer preferred to oviposit and attack fruits located on the upper half of the trees. The majority of the eggs were laid on the fruit pedicel whereas the damage was mainly located on the lower half of the fruits. Trichogrammatids were the most constant and abundant parasitoids found in both localities throughout the study period.A broca-do-abacate, Stenoma catenifer (Lepidoptera: Elachistidae, continua sendo fator limitante para o cultivo do abacate em vários estados do Brasil, nos últimos anos. Isso se deve a falta de métodos seguros e viáveis para reduzir os prejuízos causados pela praga. Com o intuito de obter informações sobre a sua bioecologia e ação de inimigos naturais, para auxiliar na elaboração de estratégias de controle, realizaram-se coletas quinzenais de 20 frutos ao acaso (10 da metade superior e 10 da metade inferior em 10 plantas, em pomar comercial, cv. Margarida, nos municípios de Arapongas e Cambé, PR, durante os meses de outubro/2001 a setembro/2002. Em laboratório determinaram-se a porcentagem

  17. Reproductive parameters of the southern stingray Dasyatis americana in southern gulf of Mexico Parámetros reproductivos de la raya látigo americana Dasyatis americana en el sur del golfo de México

    Edith Ramírez-Mosqueda

    2012-07-01

    Full Text Available The southern stingray Dasyatis americana (Hildebrand & Schroeder, 1928 is the most landed elasmobranch by small-scale fleets in southern gulf of Mexico. However, little is known of its life history parameters in this region. In this study, a total of 900 specimens were collected from February 2006 to December 2008 to determine the reproductive parameters needed for population assessments by means of ecological risk assessments or demographic analysis. Results suggested that females of D. americana reproduce annually, with a gestation of 7-8 months. The reproductive cycle of females is asynchronous, with ovulation and parturition occurring throughout the year. Females and males matured at 764 and 517 mm disc width (DW50 respectively. D. americana has one of the highest fecundity among dasyatids, from 2 to 7 embryos, with a sex ratio of embryos of 1:1. A linear relationship between maternal DW and fecundity was estimated, the larger females contain more embryos. The status of the population of D. americana is a cause of concern in the southern gulf of Mexico due to its high frequency of capture in artisanal fisheries and its apparently low biological productivity.La raya látigo americana Dasyatis americana (Hildebrand & Schroeder, 1928, conocida como balá en México, es el elasmobranquio más capturado y desembarcado por flotas artesanales en el sur del golfo de México. Sin embargo, se conoce poco de sus parámetros de historia de vida en esta región. En este estudio, fueron analizados 900 ejemplares entre febrero 2006 y diciembre 2008 para determinar los parámetros reproductivos necesarios para evaluaciones poblacionales por medio de análisis demográficos o evaluaciones de riesgo ecológico. Los resultados sugieren que las hembras de D. americana se reproducen anualmente, con una gestación de 7-8 meses aproximadamente. El ciclo reproductivo de las hembras es asincrónico, con la ovulación y alumbramiento ocurriendo a lo largo de todo el

  18. Protoplast-to-plant regeneration of American elm (Ulmus americana).

    Jones, A M P; Shukla, M R; Biswas, G C G; Saxena, P K

    2015-05-01

    This study describes a protocol for regeneration of plants from cell suspension-derived protoplasts of American elm (Ulmus americana). Efficient protoplast isolation was achieved from a two-phase culture system through the incorporation of 100 μM 2-aminoindan-2-phosphonic acid, with a yield of approximately 2 × 10(6) protoplasts/ml packed cell volume. Isolated protoplasts failed to survive in liquid or alginate bead culture systems but initiated and continued to divide when embedded in low melting point agarose beads. Protoplast-derived callus proliferated and differentiated into shoot buds in response to 10 or 20 μM thidiazuron. Differentiated buds elongated and continued to proliferate on elm shoot medium supplemented with 3.0 μM GA3. The protoplast-derived shoots rooted and acclimatized to greenhouse conditions and continued to grow. This system provides the first protoplast-to-plant regeneration system for American elm and provides a framework for the development of protoplast fusion or genome editing technologies.

  19. Paul Strand y las paradojas de la modernidad americana

    Vicente Sánchez Biosca

    2010-06-01

    Full Text Available As contribuições de Strand para o cinema estão estreitamente relacionadas com seus trabalhos fotográficos da época e mesmo com um olhar que, apesar de suas múltiplas diferenças, possui muitos pontos em comum. Se em Manhatta (1920, Strand (em colaboração com Sheeler oferece uma síntese da natureza e da modernidade, em Redes (1934 o que predomina, em contrapartida, é a experiência da arte muralista mexicana e a influência de Eisenstein (em suas andanças por México são os fatores que determinam seus esforços na construção de uma épica dos operários. Mas sem dúvida, o projeto mais ambicioso de Strand (ao mando da produtora Frontier Films foi Native Land, o filme que atendeu às demandas do American Popular Front e as moldou nas palavras dos fundadores da pátria americana.

  20. Pequeno surto de leishmaniose tegumentar americana em Macuco (Cordeiro - RJ

    Jacqueline A. Menezes

    1974-06-01

    Full Text Available Por ocasião de um pequeno surto de leishmaniose tegumentar americana em localidade do município de Cordeiro (RJ foram documentados 14 casos. Entre os fatos que chamaram a atenção dos autores estão a alta ocorrência em crianças (57%, a freqüência do componente linfangítico (40%, a tendência à cicatrização espontânea e a distribuição peri-domiciliar dos casos. Um inquérito pela intradermoreação de Montenegro na área de maior concentração de casos, realizado numa fazendo e numa escola, mostrou 17% de reações positivas, 17% de duvidosas e 66% de reações negativas, num total de 117 indivíduos testados. Não foram encontrados casos antigos cicatrizados, o que sugere um foco recente. Discute-se o significado das reações duvidosas.

  1. Avocado (Persea americana) seed as a source of bioactive phytochemicals.

    Dabas, Deepti; Shegog, Rachel M; Ziegler, Gregory R; Lambert, Joshua D

    2013-01-01

    The pulp of avocado (Persea americana, Lauraceae) has been reported to have beneficial cardiovascular health effects. Avocado oil is used for dermatological applications and its unsaponifiable portion is reported to have beneficial effects against osteoarthritis. Although the seed represents a considerable percentage of the total fruit, scientific research on the phytochemistry and biological effects of avocado seeds is in the nascent stages,. Currently, the seed represents an under-utilized resource and a waste issue for avocado processors. There is ethno-pharmacological information on the use of seeds for the treatment of health-related conditions, especially in South American countries where avocados are endemic and currently grown on a large scale. Current research has shown that avocado seeds may improve hypercholesterolemia, and be useful in the treatment of hypertension, inflammatory conditions and diabetes. Seeds have also been found to possess insecticidal, fungicidal, and anti-microbial activities. The avocado seeds and rich in phenolic compounds, and these may play a role in the putative health effects. Historically, extracts of avocado seeds were also used as ink for writing and research in our laboratory has explored the potential colorant properties of a polyphenol oxidase-produced colored avocado seed extract. Here, we review the currently-available data on the bioactivity and other functional properties of avocado seeds. We discuss the strength of the available data, the putative active compounds, and potential directions for future studies.

  2. Relationships of bradyrhizobia from the legumes Apios americana and Desmodium glutinosum.

    Parker, M A

    1999-11-01

    Multilocus enzyme electrophoresis, partial 23S rRNA sequences, and nearly full-length 16S rRNA sequences all indicated high genetic similarity among root-nodule bacteria associated with Apios americana, Desmodium glutinosum, and Amphicarpaea bracteata, three common herbaceous legumes whose native geographic ranges in eastern North America overlap extensively. A total of 19 distinct multilocus genotypes (electrophoretic types [ETs]) were found among the 35 A. americana and 33 D. glutinosum isolates analyzed. Twelve of these ETs (representing 78% of all isolates) were either identical to ETs previously observed in A. bracteata populations, or differed at only one locus. Within both 23S and 16S rRNA genes, several isolates from A. americana and D. glutinosum were either identical to A. bracteata isolates or showed only single nucleotide differences. Growth rates and nitrogenase activities of A. bracteata plants inoculated with isolates from D. glutinosum were equivalent to levels found with native A. bracteata bacterial isolates, but none of the three A. americana isolates tested had high symbiotic effectiveness on A. bracteata. Phylogenetic analysis of both 23S and 16S rRNA sequences indicated that both A. americana and D. glutinosum harbored rare bacterial genotypes similar to Bradyrhizobium japonicum USDA 110. However, the predominant root nodule bacteria on both legumes were closely related to Bradyrhizobium elkanii.

  3. Dispersal of invasive Phytolacca americana seeds by birds in an urban garden in China.

    Li, Ning; Yang, Wen; Fang, Shubo; Li, Xinhai; Liu, Zhanchen; Leng, Xin; An, Shuqing

    2017-01-01

    Although seed dispersal is a key process determining the regeneration and spread of invasive plant populations, few studies have explicitly addressed the link between dispersal vector behavior and seedling recruitment to gain insight into the invasion process within an urban garden context. We evaluated the role of bird vectors in the dispersal of pokeweed (Phytolacca americana), a North American herb that is invasive in urban gardens in China. Fruiting P. americana attracted both generalist and specialist bird species that fed on and dispersed its seeds. The generalist species Pycnonotus sinensis and Urocissa erythrorhyncha were the most frequent dispersers. Seedling numbers of P. americana were strongly associated with the perching behavior of frugivorous birds. If newly recruited bird species use seedling-safe perching sites, the P. americana will regenerate faster, which would enhance its invasive potential. Based on our observations, we conclude that the 2 main bird vectors, P. sinensis and U. erythrorhyncha, provide potential effective dispersal agents for P. americana. Our results highlight the role of native birds in seed dispersal of invasive plants in urban gardens.

  4. Susceptibility of Porphyromonas gingivalis and Streptococcus mutans to Antibacterial Effect from Mammea americana

    Alejandra Herrera Herrera

    2014-01-01

    Full Text Available The development of periodontal disease and dental caries is influenced by several factors, such as microorganisms of bacterial biofilm or commensal bacteria in the mouth. These microorganisms trigger inflammatory and immune responses in the host. Currently, medicinal plants are treatment options for these oral diseases. Mammea americana extracts have reported antimicrobial effects against several microorganisms. Nevertheless, this effect is unknown against oral bacteria. Therefore, the aim of this study was to evaluate the antibacterial effect of M. americana extract against Porphyromonas gingivalis and Streptococcus mutans. For this, an experimental study was conducted. Ethanolic extract was obtained from seeds of M. americana (one oil phase and one ethanolic phase. The strains of Porphyromonas gingivalis ATCC 33277 and Streptococcus mutans ATCC 25175 were exposed to this extract to evaluate its antibacterial effect. Antibacterial activity was observed with the two phases of M. americana extract on P. gingivalis and S. mutans with lower MICs (minimum inhibitory concentration. Also, bactericidal and bacteriostatic activity was detected against S. mutans, depending on the concentration of the extract, while on M. americana extract presented only bacteriostatic activity against P. gingivalis. These findings provide important and promising information allowing for further exploration in the future.

  5. Study on Flowering, Cultivation, and Reproduction of Agave americana Var. Marginata Hort. (Agavaceae)%Study on Flowering, Cultivation, and Reproduction of Agave americana Var. Marginata Hort. (Agavaceae)

    Fangnong LIU; Shicheng PENG; Lianren LIU

    2012-01-01

    Agave americana Var. Marginata Hort. (Agavaceae) is a perennial herb. This paper summarized its classification, distribution, morphological characteristics, habit, flowering and fruiting, cultivation and reproduction, as well as multiple uses in daily life, which provided references for the exploitation and utilization of Agave americana Vat. Marginata Hort. (Agavaceae).

  6. Leishmaniose tegumentar americana no Estado do Acre, Brasil

    Silva Natal S da

    1999-01-01

    Full Text Available OBJETIVO: Apresentar levantamento estatístico descritivo da leishmaniose tegumentar americana (LTA no Estado do Acre. MÉTODOS: Os dados obtidos foram extraídos dos formulários da "Campanha Contra a Leishmaniose", no período de janeiro de 1992 a dezembro de 1997. As variáveis estudadas foram submetidas a análise estatística descritiva. RESULTADOS: O total de casos registrados foi de 2.557. Foi adotada a divisão do Acre em meso e microrregiões para apresentação dos resultados. A maior prevalência foi na microrregião de Brasiléia (231,8 casos/10.000 hab.. A forma clínica predominante foi cutânea (84,05%. A maior ocorrência foi no sexo masculino (71,02%. Portadores com idade de até 24 anos corresponderam a 50% dos casos. Há uma predominância nas ocupações rurais. O exame clínico foi usado para diagnosticar 83,97% dos casos. A maior média de tempo de espera para procurar tratamento médico foi registrada na mesorregião do Vale do Juruá (10,37 meses. CONCLUSÃO: Os altos índices de LTA cutâneo e cutâneo-mucosa encontrados sugerem a necessidade de serem feitas pesquisas sobre os reflexos psicossociais e para identificar fatores que influem na demora do tratamento dos casos.

  7. Fecal corticoid monitoring in whooping cranes (Grus americana) undergoing reintroduction

    Hartup, Barry K.; Olsen, Glenn H.; Czekala, Nancy M.

    2005-01-01

    We used radioimmunoassay to determine fecal corticoid concentrations and assess potential stress in 10 endangered whooping cranes (Grus americana) undergoing reintroduction to the wild. Fecal samples were collected shortly after hatching at a captive facility in Maryland, during field training in Wisconsin, and throughout a human-led migration to Florida. After a 14-day decline following hatching, fecal corticoid concentrations stabilized at baseline levels for the duration of the captive period, despite exposure to potentially stressful stimuli. Shipment of the cranes to the field training site was correlated with an eight- to 34-fold increase in fecal corticoid concentrations, which returned to baseline levels within 1 week. Increases were positively correlated with age but not body weight at the time of shipping. Fecal corticoid concentrations during the training period increased slightly and exhibited greater variation than levels observed at the captive facility, but were well within expected norms based on previous studies. Fecal corticoid concentrations increased twofold following premigration physical examinations and placement of radiotransmitters, and persisted for up to 4 days before they returned to baseline levels. Though fecal corticoid concentrations and variation during the migration period were similar to training levels, there was an overall decline in fecal corticoid concentrations during the artificial migration. Acute stressors, such as capture, restraint, and severe storms, were associated with stress responses by the cranes that varied in accordance with lasting physical or psychological stimuli. The overall reintroduction process of costume-rearing, ultralight aircraft habituation, training, and artificial migration was not associated with elevations in fecal corticoid concentrations suggestive of chronic stress.

  8. Cardiovascular effects of Persea americana Mill (Lauraceae) (avocado) aqueous leaf extract in experimental animals.

    Ojewole, J A O; Kamadyaapa, D R; Gondwe, M M; Moodley, K; Musabayane, C T

    2007-01-01

    The cardiovascular effects of Persea americana Mill (Lauraceae) aqueous leaf extract (PAE) have been investigated in some experimental animal paradigms. The effects of PAE on myocardial contractile performance was evaluated on guinea pig isolated atrial muscle strips, while the vasodilatory effects of the plant extract were examined on isolated portal veins and thoracic aortic rings of healthy normal Wistar rats in vitro. The hypotensive (antihypertensive) effect of the plant extract was examined in healthy normotensive and hypertensive Dahl salt-sensitive rats in vivo. P americana aqueous leaf extract (25-800 mg/ml) produced concentration-dependent, significant (p americana leaf could be used as a natural supplementary remedy in essential hypertension and certain cases of cardiac dysfunctions in some rural Africa communities.

  9. Anticonvulsant effect of Persea americana Mill (Lauraceae) (Avocado) leaf aqueous extract in mice.

    Ojewole, John A O; Amabeoku, George J

    2006-08-01

    Various morphological parts of Persea americana Mill (Lauraceae) (avocado) are widely used in African traditional medicines for the treatment, management and/or control of a variety of human ailments, including childhood convulsions and epilepsy. This study examined the anticonvulsant effect of the plant's leaf aqueous extract (PAE, 50-800 mg/kg i.p.) against pentylenetetrazole (PTZ)-, picrotoxin (PCT)- and bicuculline (BCL)-induced seizures in mice. Phenobarbitone and diazepam were used as reference anticonvulsant drugs for comparison. Like the reference anticonvulsant agents used, Persea americana leaf aqueous extract (PAE, 100-800 mg/kg i.p.) significantly (p Persea americana leaf aqueous extract possesses an anticonvulsant property, and thus lends pharmacological credence to the suggested ethnomedical uses of the plant in the management of childhood convulsions and epilepsy.

  10. Fruit characterization of Venezuelan avocado varieties of medium oil content Caracterização de variedades venezuelanas de abacate com teor médio de óleo

    Vicente Manuel Gómez-López

    2000-12-01

    Full Text Available The objective of the present paper was to characterize some avocado fruit varieties to increase the information pool for this fruit. Twelve avocado varieties of medium oil content (8.09-11.12% from a Venezuelan orchard: Wilson Popenoe, Figueroa 1, Waldin, Puebla, Schaff, Celia, Araira FM, Adolfo, Esencia de la Vega, Lawhon, Winslowson, and Lujo were characterized for pulp oil and moisture; weight (whole fruit, seed, pulp, and peel; length, width, and fruit shape; peel characteristics (roughness, color and hand peeling; and ripeness time. Differences in oil content, moisture content and fruit weight were found when compared with results reported by other authors. The variety Puebla was the lightest (107 g and the smallest (7.90 x 4.62 cm, while Wilson Popenoe presented the highest pulp proportion (82%. Most of the varieties were ovate, with rough green peel and easy to hand peel. Peak ripeness of some varieties was at 3-12 days. Potential yields of two varieties were higher that the Venezuelan average.O objetivo do presente trabalho foi caracterizar algumas variedades de abacate para aumentar o banco de informações desta fruta. Doze variedades de teor médio de óleo (8,09 - 11,12% de um pomar da Venezuela: Wilson Popenoe, Figueroa 1, Waldin, Puebla, Schaff, Celia, Araira FM, Adolfo, Esencia de la Veja, Lawhon, Winslowson e Lujo, foram caracterizadas em relação à umidade e teor de óleo da polpa; pesos (fruta inteira, semente, polpa, casca; comprimento, largura e forma da fruta; carcterísticas da casca (rugosidade, cor, descascamento manual; tempo de maturação. Diferenças de umidade, teor de óleo e peso foram encontradas em relação a resultados de outros autores. A variedade Puebla foi a mais leve (107 g e a menor (7,90 x 4,632 cm, enquanto a Wilson Popenoe apresentou a maior proporção de polpa (82%. A maioria das variedades é oval, com pele verde e rugosa, de descascamento manual fácil. Produtividades potenciais de duas

  11. Gerenciamento de resultados e remuneração dos executivos nas companhias latino-americanas

    Santana, Alex Fabiano Bertollo

    2015-01-01

    O objetivo deste estudo foi verificar se as formas de remuneração dos executivos das Companhias Latino-Americanas influenciam o gerenciamento de resultados contábeis. Realizou-se uma análise empírica de 48 empresas, de 4 diferentes países latino-americanos em um período de 5 anos (entre 2002 e 2006), perfazendo um total de 240 observações. Para verificar a ocorrência de gerenciamento de resultados nas companhias latino-americanas, foi adotado o modelo Kang e Sivaramakrishnan (1995). Os testes...

  12. Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americana Mill., c.v. Hass

    Eduardo Padilla-Camberos

    2013-01-01

    Full Text Available The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products. Persea americana seeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract of Persea americana was evaluated with respect to its genotoxic potential through micronucleus assay in rodents. The frequency of micronuclei in groups of animals treated with avocado seed extract showed no differences compared to the negative control (vehicle; therefore, it is considered that the avocado seed extract showed no genotoxic activity in the micronucleus test.

  13. Acute toxicity and genotoxic activity of avocado seed extract (Persea americana Mill., c.v. Hass).

    Padilla-Camberos, Eduardo; Martínez-Velázquez, Moisés; Flores-Fernández, José Miguel; Villanueva-Rodríguez, Socorro

    2013-01-01

    The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products. Persea americana seeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract of Persea americana was evaluated with respect to its genotoxic potential through micronucleus assay in rodents. The frequency of micronuclei in groups of animals treated with avocado seed extract showed no differences compared to the negative control (vehicle); therefore, it is considered that the avocado seed extract showed no genotoxic activity in the micronucleus test.

  14. El Admirarse como forma de enfrentar la nueva realidad americana

    Álvarez Moreno, Raúl

    2004-12-01

    Full Text Available The article is a study of the use of admiratio to display the new American reality at the end of the fourteenth and into the fifteenth century. After exploring the semantic wealth of the term and defining it, we will present it as the first stage of the cognitive process of the human being, whose object will be Nature, understood as a whole entity in which men are included. The depiction of the admirable thing as a part of the cognitive process will also have its place in this study. But it is under the renovation that proposed the Renaissance, when admiratio turned into a poetic function related to that of movere, solving America as inventio the problem of verisimilitude inherited from the Middle Ages. Then, this concept will be used by our authors, not only to reflect a stage of knowledge, but with a clearly rhetorical function: to create a specific state of mind in the reader in order to obtain something from him.

    El artículo analiza el uso de la admiratio para presentar la nueva realidad americana a finales del siglo XV y durante el XVI. Tras explorar la riqueza semántica del término y definirlo, se parte del mismo, como el primer estadio del proceso cognitivo del ser humano, cuyo objeto será una naturaleza, entendida como totalidad que incluye al hombre. La plasmación de lo admirable como parte del proceso cognitivo tiene también su lugar en el estudio. Pero será la transformación que supone el Renacimiento, la que convertirá a la admiratio en una función poética relacionada con el movere, y a la que América como inventio solucionaba el problema de la verosimilitud heredado de la Edad Media. El concepto será usado por nuestros autores, no ya para reflejar una fase del conocimiento, sino con una función claramente retórica: provocar un estado de ánimo en el lector para conseguir algo de él.

  15. Description of a new coccid (Hemiptera, Coccidae on avocado (Persea americana Mill. from Colombia, South America

    Takumasa Kondo

    2010-04-01

    Full Text Available A new soft scale insect, Bombacoccus aguacatae Kondo, gen. n. and sp. n. (Hemiptera: Coccidae collected on the branches and twigs of avocado, Persea americana Mill. (Lauraceae in Colombia, is described and illustrated based on the adult female. An updated taxonomic key to closely related genera of the Toumeyella-group is provided.

  16. Molecular Characterization and Genetic Structure in Avocado (Persea americana Mill.) Using Simple Sequence Repeat (SSR) Markers

    Avocado (Persea americana Mill.) is an economically important tropical fruit native to Mesoamerica. It belongs to the Lauraceae family and is subdivided in three horticultural races (Guatemalan, Mexican, and West Indian) based primarily on ecological adaptation, botanical and physiological traits. T...

  17. Race, Interracial Admixture and Genetic Differentiation of Avocado (Persea americana Mill)

    Avocado (Persea americana Mill.) is a major tropical fruit crop native to Mesoamerica and domesticated around 8000 to 7000 BC. It is a member of the Laureace family and currently classified into three subspecies or races: Guatemalan (G), Mexican (M) and West Indian (WI) according to their ecological...

  18. The Scirtothrips perseae species-group (Thysanoptera), with one new species from avocado, Persea americana.

    Mound, Laurence A; Hoddle, Mark S

    2016-02-12

    Following recent molecular studies on avocado thrips, a new species is described from Costa Rica, Ecuador, and Colombia from the young leaves of avocado, Persea americana. Scirtothrips hansoni sp.n. is closely related to the Californian pest, S. perseae, and also to S. astrictus from Costa Rica that remains known from a single female. An illustrated key to these three species is provided.

  19. APPLICATION OF PROPICONAZOLE IN MANAGEMENT OF LAUREL WILT DISEASE IN AVOCADO (Persea americana Mill.) TREES

    Laurel wilt is a vascular disease of Lauraceous plants caused by a fungus (Raffaelea spp.) that is carried by a recently introduced, nonnative ambrosia beetle (Xyleborus glabratus). The disease is devastating to Persea species including redbay (Persea borbonia) and avocado (Persea americana) trees i...

  20. Applications of Microencapsulated Bifidobacterium Longum with Eleutherine Americana in Fresh Milk Tofu and Pineapple Juice

    Atchara N Phoem; Suphitchaya Chanthachum; Supayang P Voravuthikunchai

    2015-01-01

    Bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. Microencapsulation of B. longum with Eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. Microencapsulated B. longum with the extract and oligosaccharides extract in the food products showed better ...

  1. Chemotaxonomical researches in higher plants XII. Researches concerning the pigments and glucides of Phytolacca Americana L.

    Gavrila NEAMTU

    1979-08-01

    Full Text Available The paper presents the results of biochemical analyses of Phytolacca Americana plants grown in the Agrobotanical Garden Cluj-Napoca. Carotenoid pigments (neoxanthin, violaxanthin, zeaxanthin, lutein, cryptoxanthin, alpha-carotene and beta-carotene chlorophyll pigments (a,b and glycoside pigments (betalaines, quercetin have been analysed in fruits and leaves respectively.

  2. Isolation and characterization of an utero-active compound from Agave americana.

    Basilio, C M; Seyler, L; Bernstein, J; Castro de la Mata, R

    1989-12-01

    Crude extracts of Agave americana contain two utero-active compounds. One of these, tentatively named "Fraction B", has been purified to chromatographic homogeneity. Its pharmacological actions are similar to those of acetylcholine. However its chromatographic and electrophoretic mobilities are different. Some chemical properties of fraction B are compatible with the structure of an acyl derivative of choline different from acetylcholine.

  3. Phytobezoar from the stem ("quiote") of the cactus Agave americana: report of case.

    Villarreal, R; Martinez, O; Berumen, U

    1985-11-01

    Agave americana is a cactus growing abundantly in Mexico. Its cooked stem ("quiote") yields by mastication a sweet juice which is swallowed, while the fibers ("bagazo") are spit out. That is the way Mexicans are taught to chew quiote since their early childhood, and it accounts for the rarity of bezoars from this origin. One of such cases is reported herein.

  4. A Vitória Democrata e a Política Externa Americana

    GUNTHER RUDZIT

    2010-12-01

    Full Text Available O presente artigo busca analisar os impactos que a vitória do partido democrata nas eleições parlamentares de meio de mandato, realizadas em novembro, exercerão sobre a contestada política externa norte-americana levada a cabo pelo presidente Bush.

  5. Two New Spirostanol Steroidal Sapogenins from Fermented Leaves of Agave americana

    2003-01-01

    Two new spirostanol sapogenins named agavegenin A and B were isolated from the fermented leaves of Agave americana L.Their structures were elucidated as (23S, 25R)-5α- spirostan-3β, 6α, 11α, 23-tetraol (1) and (23S, 25S)-5α-spirostan-3β, 23, 27-triol (2) by spectral methods.

  6. Developmental ecdysteroid titers and DNA puffs in larvae of two sciarid species, Rhynchosciara americana and Rhynchosciara milleri (Diptera: Sciaridae).

    Soares, M A M; Hartfelder, K; Tesserolli de Souza, J M; Stocker, A J

    2015-10-01

    Ecdysteroid titers, developmental landmarks and the presence of prominent amplifying regions (DNA puffs) have been compared during late larval to pupal development in four groups of Rhynchosciara americana larvae and in R. americana and Rhynchosciara milleri. Three prominent DNA puffs (B2, C3 and C8) expand and regress sequentially on the rising phase of the 20-hydroxyecdysone (20E) titer in R. americana as a firm, cellular cocoon is being constructed. A sharp rise in 20E coincides with the regression of these puffs. The shape of the 20E curve is similar in R. milleri, a species that does not construct a massive cocoon, but the behavior of certain DNA puffs and their temporal relationship to the curve differs. Regions corresponding to B2 and C3 can be identified in R. milleri by banding pattern similarity with R. americana chromosomes and, in the case of B2, by hybridization to an R. americana probe. A B2 puff appears in R. milleri as the 20E titer rises but remains small in all gland regions. A puff similar to the R. americana C3 puff occurs in posterior gland cells of R. milleri (C3(Rm)) after the B2 puff, but this site did not hybridize to R. americana C3 probes. C3(Rm) incorporated (3)H-thymidine above background, but showed less post-puff DNA accumulation than C3 of R. americana. R. americana C8 probes hybridized to a more distal region of the R. milleri C chromosome that did not appear to amplify or form a large puff. These differences can be related to developmental differences, in particular differences in cocoon construction between the two species.

  7. Management of tremor in medieval Persia.

    Zargaran, Arman; Zarshenas, Mohammad M; Mehdizadeh, Alireza; Mohagheghzadeh, Abdolali

    2013-01-01

    Tremor has been described in traditional systems of medicine throughout history. Persian medicine was one of those systems in medieval times and in it neurology and neurosurgery were also widely practiced and accepted. Based on the main Persian medical manuscripts, the current study focuses on the medieval concept of tremor as an important neurological disorder in order to clarify the development of neurology. Accordingly, three main approaches to the control and treatment of tremor in traditional Persian medicine are considered. First is lifestyle modification. The administration of simple medicines is the second, and the last is the application of compound medicines. Our study shows how much was known about tremor in traditional Persian medicine.

  8. Evaluation of Piper aduncum Linn. Essential Oil (Fam:Piperaceae against Periplaneta americana(L.

    I Ling A

    2009-12-01

    Full Text Available Background: The efficacy of Piper aduncum essential oil was evaluated against Periplaneta americana adults and nymphs in the laboratory.Methods: The plant essential oil at varying concentrations ranging between 10,000 to 80,000 ppm were placed inside glass beakers, rolled horizontally to ensure the essential oil covers all sides of the beakers and exposed to adults and nymphs of P. americana. Resigen (R 1ppm was used as positive control and distilled water as negative control. The LT50 and LT90 was obtained using Log Probit programme.Results: Exposure of essential oil to females P. americana at concentrations between 10,000 to 80,000 ppm indicated the LT50 and LT90 values between 5.31 h-189.19 h and 14.90 h-2105.31 h, respectively. Treatment with the same con­centrations against males P. americana ,the LT50 and LT90 were 2.08 h-181.73 h and 5.4 h-8460.51 h, respectively. Treatment against the nymphal stage with the same range of concentrations indicated the LT50 and LT 90 of 4.68 h-381.02 h and 28.71 h-5313.36 h, respectively.The nymphs and males were more susceptible than the females cock­roaches. Treatment with Resigen (R at 1ppm indicated much lower LT 50 and LT 90 values of 2.54 h-9.47 h for the fe­males, 1.47 h-4.22 h for the males and 4.69 h-8.92 h for the nymphs.The negative control indicated no mortality for all stages of the cockroach.Conclusion: Piper aduncum essential oil can be used as an alternative natural product for controlling the cockroach Peri­patetic americana.

  9. Identification of two capa cDNA transcripts and detailed peptidomic characterization of their peptide products in Periplaneta americana

    Susanne Neupert

    2014-06-01

    Full Text Available The first CAPA peptides of insects were identified from Periplaneta americana using extracts of 8000 abdominal perisympathetic organs. The corresponding gene of cockroaches and other basal insects, however, remained undiscovered. Here we identified two capa cDNA transcripts of P. americana. Single cell mass spectrometry of capa neurons in abdominal ganglia yielded a complete coverage of the precursor sequences of both transcripts; with intermediates of precursor processing more prominent in somata than in perisympathetic organs. The processing of the CAPA precursor in P. americana is compared with that in the beetle Tribolium castaneum which was re-evaluated in this study.

  10. Postharvest of ‘Fuerte’ and ‘Hass’ avocados: physical and chemical characteristics, damages and control of diseases/ Pós-colheita de abacates ‘Fuerte’ e ‘Hass’: características físicas e químicas, danos e controle de doenças

    Maria Cecília Arruda

    Full Text Available Postharvest disease is considered an important cause of avocado depreciation during its commercialization. This work aimed to evaluate the postharvest damages and the physical chemical characteristics of avocados ‘Fuerte’ and ‘Hass’, processed at the packinghouse, and to evaluate the effect of products for postharvest disease control. The characteristics skin color, firmness, titratable acidity and soluble solids content and the incidence of the postharvest damages were evaluated periodically in avocados sampled in three different stages in a packinghouse (arrival, pallet and processed fruits after storage for 30 days at 5ºC. For diseases control, ‘Hass’ avocado were treated by immersion with the following products: azoxystrobin, benzalkonium chloride, chlorine dioxide, Ecolife®, sodium hypochlorite, imazalil, prochloraz and thiabendazole. In general, the highest physical chemical changes were observed in fruits collected at their arrival to the packinghouse and in processed fruits after stored for 30 days at 15 days of storage at 25°C. The rots incidence was 56,7% in ‘Fuerte’ and 75,7% in ‘Hass’. Lower incidence of rots was observed in fruits sampled at the pallet and a higher incidence in the processed fruits, after refrigerated storage. Anthracnose was the most important disease in both cultivars. The mechanical injuries increased with the processing stages; however, they did not influence the incidence of the rots. Procloraz and imazalil were the most efficient fungicides in reducing the incidence of rots. Doença pós-colheita é considerada uma importante causa de desvalorização do abacate por ocasião da comercialização. Este trabalho objetivou avaliar os danos pós-colheita e as características físicas e químicas de abacates ‘Fuerte’ e ‘Hass’, beneficiados em packinghouse, e o efeito de produtos no controle pós-colheita das podridões. As características cor da casca, firmeza, acidez titulável e s

  11. Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.

    Neyser De La Torre-Ruiz; Víctor Manuel Ruiz-Valdiviezo; Clara Ivette Rincón-Molina; Martha Rodríguez-Mendiola; Carlos Arias-Castro; Federico Antonio Gutiérrez-Miceli; Héctor Palomeque-Dominguez; Reiner Rincón-Rosales

    2016-01-01

    ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRN...

  12. Pathogenicity of Aspergillus westerdijkiae to females and oothecae of Periplaneta Americana

    Mariah Valente Baggio

    2016-01-01

    Full Text Available ABSTRACT: Cockroach control is performed by the application of chemical insecticides which exert high selective pressure on populations and introduces synthetic substances in the environment, motivating the search for other methods of control such as entomopathogenic fungi. The objectives of this study were to investigate the pathogenicity of the JAB 42 Aspergillus westerdijkiae to females and oothecae of Periplaneta americana and to demonstrate its mechanism of action on oothecae. Suspensions containing 106 to 108 conidia/ml were used to infect females and oothecae. Mortality and other variables such as scanning electron microscopy were used to demonstrate the mechanism of action of the fungus. The isolated JAB 42 A. westerdijkiae is pathogenic to oothecae of P. americana, with low capacity to kill females. Adhesion, germination, penetration and extrusion of the fungus on the cockroach oothecae were observed.

  13. Leishmaniose tegumentar americana: apresentação pouco comum American tegumentary leishmaniasis: an unusual presentation

    Antonio Carlos Martins Guedes

    2008-10-01

    Full Text Available Relata-se caso de leishmaniose tegumentar americana com lesões proeminentes na face. Os critérios diagnósticos incluíram dados clínicos e epidemiológicos, intradermorreação de Montenegro, identificação de Leishmania pela reação em cadeia da polimerase e resposta clínica ao tratamento. A importância do relato se deve ao fato de se tratar de caso incomum de leishmaniose tegumentar americana.We report the case of a patient with American cutaneous leishmaniasis and prominent lesions on the face. Diagnostic criteria included clinical and epidemiological data, Montenegro's skin test, identification of Leishmania by means of polymerase chain reaction and clinical response to treatment. Our report is important to call attention to an unusual presentation of American cutaneous leishmaniasis.

  14. Secondary metabolites from the unripe pulp of Persea americana and their antimycobacterial activities.

    Lu, Ying-Chen; Chang, Hsun-Shuo; Peng, Chien-Fang; Lin, Chu-Hung; Chen, Ih-Sheng

    2012-12-15

    The fruits of Persea americana (Avocado) are nowadays used as healthy fruits in the world. Bioassay-guided fractionation of the active ethyl acetate soluble fraction has led to the isolation of five new fatty alcohol derivatives, avocadenols A-D (1-4) and avocadoin (5) from the unripe pulp of P. americana, along with 12 known compounds (6-17). These structures were elucidated by spectroscopic analysis. Among the isolates, avocadenol A (1), avocadenol B (2), (2R,4R)-1,2,4-trihydroxynonadecane (6), and (2R,4R)-1,2,4-trihydroxyheptadec-16-ene (7) showed antimycobacterial activity against Mycobacterium tuberculosis H(37)R(V)in vitro, with MIC values of 24.0, 33.8, 24.9, and 35.7 μg/ml, respectively.

  15. Applications of microencapsulated Bifidobacterium longum with Eleutherine americana in fresh milk tofu and pineapple juice.

    Phoem, Atchara N; Chanthachum, Suphitchaya; Voravuthikunchai, Supayang P

    2015-04-01

    Bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. Microencapsulation of B. longum with Eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. Microencapsulated B. longum with the extract and oligosaccharides extract in the food products showed better survival than free cells under adverse conditions. Sensory analysis demonstrated that the products containing co-encapsulated bacterial cells were more acceptable by consumers than free cells. Pineapple juice prepared with co-encapsulated cells had lower values for over acidification, compared with the juice with free cells added. This work suggested that microencapsulated B. longum with E. americana could enhance functional properties of fresh milk tofu and pineapple juice.

  16. Applications of Microencapsulated Bifidobacterium Longum with Eleutherine Americana in Fresh Milk Tofu and Pineapple Juice

    Atchara N. Phoem

    2015-04-01

    Full Text Available Bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. Microencapsulation of B. longum with Eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. Microencapsulated B. longum with the extract and oligosaccharides extract in the food products showed better survival than free cells under adverse conditions. Sensory analysis demonstrated that the products containing co-encapsulated bacterial cells were more acceptable by consumers than free cells. Pineapple juice prepared with co-encapsulated cells had lower values for over acidification, compared with the juice with free cells added. This work suggested that microencapsulated B. longum with E. americana could enhance functional properties of fresh milk tofu and pineapple juice.

  17. Vasorelaxant action of aqueous extract of the leaves of Persea americana on isolated thoracic rat aorta.

    Owolabi, Mbang A; Jaja, Smith I; Coker, Herbert A B

    2005-09-01

    The present study investigated the vasorelaxant action of the aqueous leaves extract of Persea americana on isolated rat aorta. The results showed that the extract produced significant vasorelaxation and that the effect is dependent on the synthesis or release of endothelium-derived relaxing factors (EDRFs) as well as the release of prostanoid. The extract also reduced vasoconstriction probably by inhibiting Ca2+ influx through calcium channels.

  18. Optimization of Insoluble and Soluble Fibres Extraction from Agave americana L. Using Response Surface Methodology

    Amine Bouaziz; Manel Masmoudi; Amel Kamoun; Souhail Besbes

    2014-01-01

    Experimental design methodology was used to determine significant factors affecting the extraction yield of soluble and insoluble fibres from Agave americana L. and in second time to find optimum conditions leading to the highest yield. Results clearly indicated that the temperature, the powder to water (P/W) ratio, and the agitation speed were the most important factors influencing fibres extraction yield which increased with temperature, P/W ratio, and agitation speed. Ionic strength affect...

  19. Phenotypic Variations in the Foliar Chemical Profile of Persea americana Mill. cv. Hass.

    García-Rodríguez, Yolanda Magdalena; Torres-Gurrola, Guadalupe; Meléndez-González, Claudio; Espinosa-García, Francisco J

    2016-12-01

    The Hass avocado tree Persea americana cv. Hass was derived from a single hybrid tree of P. americana var. drymifolia and P. americana var. guatemalensis, and it is propagated clonally by grafting. This cultivar is the most widely planted in the world but its profile of secondary metabolites has been studied rarely despite of its importance in plant protection. We illustrate the variability of the volatilome of mature leaves by describing the average chemical composition and the phenotypic variability found in 70 trees. Contrary to the uniformity expected in the Hass cultivar, high variability coefficients were found for most of the 36 detected foliar volatile compounds; furthermore we found six chemotypes grouping the foliar phenotypes of the sampled trees using hierarchical cluster analysis. About 48% of trees were grouped in one chemotype; five chemotypes grouped the remaining trees. The compounds that determined these chemotypes were: estragole, α-farnesene, β-caryophyllene, germacrene D, α-cubebene and eugenol. This striking variation in a cultivar propagated clonally is discussed in terms of somatic mutation.

  20. Pro-apoptotic effect of Persea americana var. Hass (avocado) on Jurkat lymphoblastic leukemia cells.

    Bonilla-Porras, Angelica R; Salazar-Ospina, Andrea; Jimenez-Del-Rio, Marlene; Pereañez-Jimenez, Andres; Velez-Pardo, Carlos

    2013-11-05

    Abstract Context: Therapy for leukemia has a limited efficacy. There is a need to search for alternative anti-leukemia therapies. Persea americana Mill var. Hass (Lauraceae) is a tropical fruit (avocado) that might be used against cancer. Objective: To investigate whether P. americana induces death in Jurkat lymphoblastic leukemia cells. Materials and methods: Four ethanol extracts (0.1, 0.5, 1, 2 and 5 mg/mL) from avocado fruit (endocarp, whole seed, seed and leaves) were analyzed against Jurkat cells. Hydrogen peroxide generation by oxidation of 2',7'-dichlorodihydrofluorescein diacetate to the fluorescent compound 2',7'-dichlorfluorescein assay, acridine orange/ethidium bromide staining, flow cytometry analysis of annexin-V/7-amino-actinomycin, mitochondrial membrane potential and immunocytochemistry detection of transcription factor p53, caspase-3 and apoptosis-inducing factor (AIF) were evaluated. Results: Endocarp, seed, whole seed, and leaf (0.1 mg/mL) extracts induced significant apoptosis in Jurkat cells (p americana extracts function as a pro-apoptotic compound. Leukemic cells are eliminated through an oxidative stress mechanism. This study contributes to the understanding of the molecular mechanism of the avocado and its therapeutic action on leukemia.

  1. One pot phytosynthesis of gold nanoparticles using Genipa americana fruit extract and its biological applications

    Kumar, Brajesh, E-mail: krmbraj@gmail.com [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador); Smita, Kumari; Cumbal, Luis [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador); Camacho, Javier [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador); Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología, C.P. 07360 Mexico City (Mexico); Hernández-Gallegos, Elisabeth; Guadalupe Chávez-López, María de [Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología, C.P. 07360 Mexico City (Mexico); Grijalva, Marcelo; Andrade, Kleber [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador)

    2016-05-01

    In this article, rapid one pot synthesis of gold nanoparticles (GNPs) using an eco-friendly extract of Genipa americana L. fruit is described. Electrospray ionization mass spectrometry (ESI-MS) and Fourier transform infrared (FTIR) spectroscopic studies demonstrated that small molecules such as genipin, genipaol, geniposide and ranolazine can act as reducer as well as stabilizers. The monodispersed, spherical GNPs were further characterized by UV–vis spectroscopy at λ{sub max} = 535 nm, transmission electron microscopy (TEM), dynamic light scattering (DLS) and X-ray diffraction (XRD) analysis. This synthetic approach offers a greener and alternate route to the preparation of GNPs free from toxic chemical components and stable for 6–7 months under room temperature. The green synthesized GNPs showed weak antioxidant efficacy against 1,1-diphenyl-2-picrylhydrazyl and no cytotoxicity against A-549 and HeLa human cancer cell lines, from lung and cervix. This study opens a new industrial scope of G. americana fruit in nanoscience and as surface modified GNPs can be developed into a successful drug carrier for future pharmaceutical products. - Highlights: • Gold nanoparticles can be synthesized by Genipa americana fruit extract. • TEM-DLS analysis confirmed that the average particle size is 30.4 ± 14.9 nm. • Nanoparticles showed weak antioxidant and no cytotoxicity activity.

  2. Plant growth-promoting rhizobacteria affect the growth and nutrient uptake of Fraxinus americana container seedlings.

    Liu, Fangchun; Xing, Shangjun; Ma, Hailin; Du, Zhenyu; Ma, Bingyao

    2013-05-01

    Plant growth-promoting rhizobacteria (PGPR) are important catalysts that regulate the functional properties of agricultural systems. However, there is little information on the effect of PGPR inoculation on the growth and nutrient accumulation of forest container seedlings. This study determined the effects of a growth medium inoculated with PGPR on the nutrient uptake, nutrient accumulation, and growth of Fraxinus americana container seedlings. PGPR inoculation with fertilizer increased the dry matter accumulation of the F. americana aerial parts with delayed seedling emergence time. Under fertilized conditions, the accumulation time of phosphorous (P) and potassium (K) in the F. americana aerial parts was 13 days longer due to PGPR inoculation. PGPR increased the maximum daily P and K accumulations in fertilized seedlings by 9.31 and 10.44 %, respectively, but had little impact on unfertilized ones. Regardless of fertilizer application, the root exudates, namely sugars, amino acids, and organic acids significantly increased because of PGPR inoculation. PGPR inoculation with fertilizer increased the root, shoot, and leaf yields by 19.65, 22.94, and 19.44 %, respectively, as well as the P and K contents by 8.33 and 10.60 %, respectively. Consequently, the N, P, and K uptakes increased by 19.85, 31.97, and 33.95 %, respectively. Hence, PGPR inoculation with fertilizer can be used as a bioenhancer for plant growth and nutrient uptake in forest container seedling nurseries.

  3. Contribuição ao estudo da Tripanosomiase Americana

    Bichat de Almeida Rodrigues

    1942-01-01

    ano (época do estío. 6 Em plena mata, n’uma toca de tamanduá (T. tetradactylus foram encontradas larvas, ninfas e adultos de P. geniculatus. A casa situada mais próximo desse foco, foi a que apresentou maior infestação (22 exemplares e exclusiva para essa espécie. Em uma toca de macaco da noite (P. flavus, foi encontrada uma larva de Triatomídeo. Este foco também ficava próximo à casa acima referida. Em toca de P. flavus foi também achado um exemplar adulto de Panstrongylus refotuberculatus. 7 Amostras de S. cruzi isoladas de animais silvestres, mostraram fraco poder infectante. A amostra isolada do cão, embora infectando facilmente os animais de laboratório, pelos estudos biométricos feito por DIAS e FREITAS, afasta-se das amostras humanas típicas. 8 São discutidos os resultados acima referidos e, pelos hábitos dos transmissores, pela predominância de depositários silvestres do parasito, conclue-se pela natureza silvestre da Tripanosomiase Americana no local estudado. Se bem que não tenham sido verificadas infecções humanas, dado o encontro de um cão parasitado – infecção esta que se pode ter verificado pelo meio normal da transmissão da moléstia – admite-se a possibilidade do aparecimento de casos humanos nessa região. Ressalta-se a confirmação que tais resultados parecem trazer á hipótese de CARLOS CHAGAS, que pensava ser esta doença primitivamente silvestre, com posterior adaptação aos animais domésticos e ao homem.1 The epidemiology of Chagas’ disease was studied for the first time in a systematized work, in a district of the region of the Amazon estuary, whose ecological conditions differ from those encountered in other zones where similar researches have been made. 2 At the place chosen for the studies – Aurá – a locality about 10 kms from Belem (State of Pará, no human infection by S. cruzi has been found, either by blood examination or xenodiagnosis, both having been tried on all the inhabitants (117 people

  4. Detecção de Salmonella Anatum em ema (Rhea americana Detection of Salmonella Anatum in the Greater Rhea (Rhea americana

    Rosecler Alves Pereira

    2008-06-01

    Full Text Available Para pesquisa de Salmonella spp. foram coletadas amostras de fígado e conteúdo cecal de 70 emas (Rhea americana abatidas no Rio Grande do Sul - Brasil. Uma colônia morfológica e bioquimicamente compatível com Salmonella spp., isolada de uma amostra de fígado, foi sorotipada como Salmonella Anatum. Considerando-se o alto potencial zoonótico deste microrganismo, destaca-se a relevância do controle microbiológico efetivo em frigoríficos que abatem espécies silvestres, assim como no produto final.In aiming to investigate the Salmonella spp. presence in one slaughterhouse in Rio Grande do Sul - Brazil, liver and cecum samples from 70 Greater Rhea (Rhea americana were collected. One Salmonella-like colonie was serologically typed and identified as Salmonella Anatum. Considering the high zoonotical potential of this microorganism, an effective microbiological control of wild animal slaughterhouses and the final product is needed.

  5. Herança e ligação em locos de isoenzimas em Genipa americana L. Inheritance and linkage in isozymes loci of Genipa americana L.

    Alexandre Magno SEBBENN

    2004-06-01

    Full Text Available A herança e a ligação foram estudadasem quatro locos polimórficos (6pghd-1, Pgi-2,Mdh-1 e Mdh-2 de três sistemas isoenzimáticos deGenipa americana, analisados por eletroforesehorizontal em gel de amido, usando tecidoscoletados de quinze progênies de polinização aberta,procedentes de Moji-Guaçu, Estado de São Paulo.Esses locos segregaram de dois a três alelos.A segregação nesses locos foi homogênea entreárvores. Contudo, existem desvios significativos dasegregação esperada 1:1 para algumas árvores emalguns locos. Desvios significativos da razão desegregação esperada 1:1 foram detectados noslocos Mdh-2 e 6pgdh-1. Não foram detectadasevidências de ligação entre nenhum dos pares delocos avaliados em G. americana e esses podemser utilizados sem restrição para estudos do sistemade reprodução, diversidade e estrutura genética depopulações da espécie.Inheritance and linkage for fourpolymorphic loci (6pghd-1, Pgi-2, Mdh-1 andMdh-2 from three enzyme systems in Genipaamericana were analyzed by horizontal starch gelelectrophoresis using tissues collected from fiftyopen-pollinated families in Moji-Guaçu, São PauloState. These allozyme loci have segregated fromtwo to four alleles per polymorphic locus. Genesegregation on those loci was homogeneous amongtrees. However, there were significant deviationsfrom the expected segregation ratios on some lociand some individual trees. In addition, Mdh-2 and6pgdh-1 loci have shown significant deviationsfrom the 1:1 expected segregation ratio. Linkagedisequilibrium evidences were not detected betweenany two pairs of loci from G. americana and thosecan be used with no restriction to study the matingsystem, genetic diversity, and genetic structure onthe species.

  6. Physical and chemical characterization of the pulp of different varieties of avocado targeting oil extraction potential Caracterização física e química da polpa de diferentes variedades de abacate visando o potencial para extração de óleo

    Edinéia Dotti Mooz

    2012-06-01

    Full Text Available The aim of this study was to evaluate the physicochemical properties of avocado pulp of four different varieties (Avocado, Guatemala, Dickinson, and Butter pear and to identify which has the greatest potential for oil extraction. Fresh avocado pulp was characterized by moisture, protein, fat, ash, carbohydrates and energy contents were determined. The carotenoids and chlorophyll contents were determined by the organic solvent extraction method. The results showed significant differences in the composition of the fruit when varieties are compared. However, the striking feature in all varieties is high lipid content; Avocado and Dickinson are the most suitable varieties for oil extraction, taking into account moisture content and the levels of lipids in the pulp. Moreover, it could be said that the variety Dickinson is the most affected by the parameters evaluated in terms of overall quality. Chlorophyll and carotenoids, fat-soluble pigments, showed a negative correlation with respect to lipids since it could be related to its function in the fruit. The varieties Avocado and Dickinson are an alternative to oil extraction having great commercial potential to be exploited thus avoiding waste and increasing farmers’ income.O objetivo deste estudo foi avaliar as características físico-químicas da polpa de abacate de quatro diferentes variedades (Avocado, Guatemala, Dicson e Manteiga, visando identificar qual possui maior potencial para extração de óleo. A polpa de abacate fresca foi caracterizada através da determinação dos teores de umidade, proteína, lipídios, cinzas, carboidratos e valor energético. Os teores de carotenoides e clorofila foram determinados através do método de extração com solvente orgânico. Os resultados mostram diferenças significativas na composição dos frutos, quando se confrontam as variedades. No entanto, a característica marcante em todas as variedades é o alto teor de lipídios, sendo as variedades

  7. Simple Y-autosomal incompatibilities cause hybrid male sterility in reciprocal crosses between Drosophila virilis and D. americana.

    Sweigart, Andrea L

    2010-03-01

    Postzygotic reproductive isolation evolves when hybrid incompatibilities accumulate between diverging populations. Here, I examine the genetic basis of hybrid male sterility between two species of Drosophila, Drosophila virilis and D. americana. From these analyses, I reach several conclusions. First, neither species carries any autosomal dominant hybrid male sterility alleles: reciprocal F(1) hybrid males are perfectly fertile. Second, later generation (backcross and F(2)) hybrid male sterility between D. virilis and D. americana is not polygenic. In fact, I identified only three genetically independent incompatibilities that cause hybrid male sterility. Remarkably, each of these incompatibilities involves the Y chromosome. In one direction of the cross, the D. americana Y is incompatible with recessive D. virilis alleles at loci on chromosomes 2 and 5. In the other direction, the D. virilis Y chromosome causes hybrid male sterility in combination with recessive D. americana alleles at a single QTL on chromosome 5. Finally, in contrast with findings from other Drosophila species pairs, the X chromosome has only a modest effect on hybrid male sterility between D. virilis and D. americana.

  8. Utilização da microscopia eletrônica de varredura como ferramenta de avaliação da estrutura do tecido de abacate 'quintal' após danos mecânicos Use of scanning electronic microscopy as tool of the tissue structure evaluation of quintal ' avocado tissue structure after mechanical injuries

    Juliana Sanches

    2007-04-01

    Full Text Available Avaliaram-se os efeitos de injúrias mecânicas, por impacto, compressão e corte nas estruturas celulares de abacates 'Quintal'. Na injúria por impacto, eles foram deixados cair, em queda livre, de uma altura de 2,00 m, sobre os lados opostos, de sua região equatorial; na compressão, foram colocados sob um peso de 117,6 N, por 24 horas, o que provocou duas lesões opostas, no sentido longitudinal; e os cortes foram aplicados em número de quatro, longitudinalmente, com 40 mm de comprimento e 4 mm de profundidade, nos lados opostos dos frutos. Após 5 dias de armazenamento, sob condições de ambiente (25ºC e 60% UR, procedeu-se à verificação das estruturas celulares através de microscopia eletrônica de varredura. A microscopia eletrônica de varredura indicou que os abacates injuriados por compressão e impacto, embora sem lesões aparentemente visíveis, apresentavam desordem celular em suas estruturas.This work aimed to evaluate the effects of mechanical injuries, by impact, compression and cut in the cellular structures of 'Quintal' avocados. In the injury by impact, they were submitted, by free fall, to a height of 2.00 m, in opposed sides of their equatorial area; in the compression, they were submitted by a weight of 117.6 N for 24 hours, and it provoked two opposite lesions in the longitudinal area; and the cuts were longitudinally applied at number of four, with 40 mm of length and 4 mm of depth in the opposed sides of the fruits. After 5 days of storage, under the laboratory conditions (25ºC and 60% RH, the verification of the cellular structures was proceeded through scanning electron microscopy, which indicated that the injuried avocados by compression and impact, although without apparently visible lesions, presented cellular disorder in their structures.

  9. Polifenoles en Myristicaceae americanas II. Distribución en especies colombianas Polifenoles en Myristicaceae americanas II. Distribución en especies colombianas

    Aguirre Gálviz Luis Enrique

    1988-06-01

    Full Text Available

    The distribution of phenolic acids and flavonoid aglycones in 65 samples of 11 species of Colombian Myristicaceae was studied in order to complete the data obtained from other samples of American Myristicaceae. The occurrence and distribution of these compounds could be used for chemotaxonomic discusions of the family.

    Se estudió la distribución de polifenoles en 65 muestras de material de herbario de II especies de Myristicaceae coleccionadas en Colombia, con el objeto de complementar los datos obtenidos en otras muestras de Myristicaceae americanas. El patrón de distribución de estos compuestos podría servir para una discusión quimiotaxonómica de la familia.

  10. Cloning of a chitinase gene from Ewingella americana, a pathogen of the cultivated mushroom, Agaricus bisporus

    P.W. Inglis

    2000-09-01

    Full Text Available We have isolated a gene encoding a chitinase (EC 3.2.1.14 from Ewingella americana, a recently described pathogen of the mushroom Agaricus bisporus. This gene, designated chiA (EMBL/Genbank/DDBJ accession number X90562, was cloned by expression screening of a plasmid-based E. americana HindIII genomic library in Escherichia coli using remazol brilliant violet-stained carboxymethylated chitin incorporated into selective medium. The chiA gene has a 918-bp ORF, terminated by a TAA codon, with a calculated polypeptide size of 33.2 kDa, likely corresponding to a previously purified and characterised 33-kDa endochitinase from E. americana. The deduced amino acid sequence shares 33% identity with chitinase II from Aeromonas sp. No. 10S-24 and 7.8% identity with a chitinase from Saccharopolyspora erythraeus. Homology to other chitinase sequences was otherwise low. The peptide sequence deduced from chiA lacks a typical N-terminal signal sequence and also lacks the chitin binding and type III fibronectin homology units common to many bacterial chitinases. The possibility that this chitinase is not primarily adapted for the environmental mineralisation of pre-formed chitin, but rather for the breakdown of nascent chitin, is discussed in the context of mushroom disease.O gene que codifica uma quitinase (EC 3.2.1.14 foi isolado de Ewingella americana, recentemente descrita como patógeno do cogumelo Agaricus bisporus. Este gene, denominado chiA (EMBL/Genebank/DDBJ número de acesso X9061, foi clonado e selecionado a partir de livraria genômica construída por digestão do DNA de E. americana com HindIII e ligação em plasmídio de expressão em E. coli, utilizando meio seletivo contendo quitina carboximetilada, corada com "remazol brilliant violet'' para seleção de clones. O gene chiA apresenta uma ORF de 918 bp, código terminador TAA, tendo o tamanho do polipeptídeo sido calculado como 33,2 kDa, o qual corresponde ao tamanho de 33 kDa da endoquitinase

  11. Vascular riffle flora of Appalachian streams: the ecology and effects of acid mine drainage on Justificia americana (L. ) Vahl

    Koryak, M.; Reilly, R.J.

    1984-06-01

    Justicia americana is a stout-based colonial plant, abundant in most of the larger, low to moderate gradient streams of the upper Ohio River basin. The distribution of J. americana is related to acid drainage from bituminous coal mining operations in the upper Ohio River drainage basin. Possible fluvial and biological consequences of the colonization or absence of Justicia are considered. Luxuriant growths were noted on gravel bars and riffles of larger, unpolluted streams in the basin. Acid mine drainage severely depresses the growth of the plant, leaving gravel shoals and riffles in the acid streams either barren or dominated by other emergent species. Particular among these new species is Elecocharis acicularis. The elimination of J. americana from suitable habitat adversely affects channel morphology, substrate composition, general aesthetic quality and aquatic stream life in the region. 16 references, 2 figures, 3 tables.

  12. Flavonoids from Tilia americana with anxiolytic activity in plus-maze test.

    Herrera-Ruiz, Maribel; Román-Ramos, Rubén; Zamilpa, Alejandro; Tortoriello, Jaime; Jiménez-Ferrer, J Enrique

    2008-07-23

    The aerial parts of Tilia americana var. mexicana (Schltdl) Hardin (Tiliaceae) have been widely used in Mexican traditional medicine to relieve sleeplessness, headache, and nervous excitement. The anxiolytic effect of four extracts and several flavonoid fractions from the bracts of Tilia americana subsp.mexicana, var. mexicana (Schltdl) Hardin or Tilia mexicana (Tiliaceae) was studied. Administration of 100mg/kg of n-hexane, ethyl acetate, and aqueous extracts to elevated plus-maze (EPM)-exposed mice displayed no anxiolytic effect; however, identical doses of methanol extract was able to increase the time percentage that mice spent in the EPM's open arms, as well as the percentage of crossings in the EPM's arms. The dose-response curve produced by methanol extract showed anxiolytic activity since 25mg/kg; animals showed no motor activity alteration in the open field test (OFT). Methanol extract was subjected to a bioassay-guided fractionation to obtain four ascendant polarity fractions (F1-F4) which were administrated at 100mg/kg. Data results indicate that F1 displayed the main anxiolytic effect. The purification of F1 produced a rich flavonoid anxiolytic mixture (F1C). This fraction was purified by RP-18 open chromatographic column to obtain four polar descent fractions: F1C(1), F1C(2), F1C(3), and F1C(4), respectively. Tiliroside was the major ingredient from the active fraction. High performance liquid chromatography analysis indicated that F1C was constituted principally of tiliroside, quercetin, quercitrin, kaempherol, and their glycosides. These results supported the use of Tilia americana in Mexican traditional medicine as well as the anxiolytic effect of a rich flavonoid fraction without affect motor activity.

  13. Antioxidant and acetylcholinesterase inhibition properties of Amorpha fruticosa L. and Phytolacca americana L.

    Dimitrina Zh Zheleva-Dimitrova

    2013-01-01

    Full Text Available Background: Amorpha fruticosa L. and Phytolacca americana L. are native plants for North America, but invasive for Central Europe and the Mediterranean areas. Previous investigation reported DPPH radical scavenging activity of A. fruticosa seeds from Mississippi river basin and P. americana berries from Iran. The aim of the present study was to investigate methanol extracts from leaves and fruits of these invasive species growing in Bulgaria for radical scavenging and acetylcholinesterase inhibitory potential. Materials and Methods: Antioxidant activity was investigated using DPPH and ABTS free radicals; FRAP assay and inhibition of lipid peroxidation in linoleic acid system by FTC. Modified Ellman′s colorimetric method was carried out to quantify acetylcholinesterase inhibition potential. In addition, the quantities of total polyphenols, flavonoids, and hydroxycinnamic derivatives were determinated using Folin-Chiocalteu reagent, AlCl 3 , and Na 2 MoO 4 , respectively. Results: The highest concentrations of total polyphenols and flavonoids were found in A. fruticosa leaves (786.70±1.78 mg/g dry extract and 32.19±0.29 mg/g dry extract, respectively. A. fruticosa fruit was found to be the most enriched in total hydroxycinnamic derivatives (153.55±1.11 mg/g dry extract and demonstrated the highest antioxidant activity: DPPH, IC 50 9.83 μg/mL; ABTS, IC 50 2.90 μg/mL; FRAP , 642.95±3.95 μg TE/mg de, and acetylcholinesterase inhibitory activity, 48.86±0.55% (2 mg/mL. Conclusions: Phytolacca americana leaves and Amorpha fruticosa could be useful in therapy of free radical pathologies and neurodegenerative disorders.

  14. THE PAINT FORMULATION IN LAMBDACYHALOTHRIN USAGE AS P. americana COCKROACH CONTROL MEASURES

    Lulus Susanti

    2014-06-01

    Full Text Available ABSTRAKKecoa adalah salah satu jenis serangga yang dianggap kotor dan merupakan seranggavektor. Scbagian besar kecoa yang ditemukan di sekitar pemukiman adalah Periplanetaamericana, Periplaneta brunea, Periplaneta australisea, Blatella germanica, Supellalongipalpa, Neostylopiga rhombifoUa dan Nauphaeta cinerea. Beberapa kecoa juga dapatmenularkan virus, bakteria, worm, protozoit dan jamur.Pengcndalian kecoa secara fogging dengan menggunakan insektisida masih kurangefektif dan praktis, sehingga dibutuhkan alternatif pengcndali. Dalam rangka untuk mencarimetode alternatif pengcndalian kecoa, maka akan dilakukan percobaan menggunakan cat kayuyang ditambahkan dengan insektisida.Dalam penelitian ini, insektisida cat kayu menggunakan bahan aktif Lambdacyhalothrin10WP. Pertama-tama dibuat beberapa solusi dari cat insektisida dalam dosis banyak. Kemudiancat papan kayu lapis. Setelah kcring sekitar satu hari, kemudian melakukan pengujian bioassayterhadap kecoa {Periplaneta americana . Hasil penelitian ini menunjukkan bahwa kayuinsektisida cat dengan dosis minimal 1,591% efektif untuk membunuh kecoa hingga 95%.Kata kunci: kecoa, cat berinsektisida, lambdacyhalothrinABSTRATCCockroaches are still considered to be disgusting insects and vector. Mostlycockroaches that are found around the resident, such as Periplaneta americana, Periplanetabrunea,Periplaneta australisea, Blatella germanica, Supella longipalpa, NeostylopigarhombifoUa and Nauphaeta cinerea. There are can transmit virus, bakteria, worm, protozoit andfungi.The methods for cocroaches control by using the fogging with insecticide is still lesseffective and practical, so it will need a controlling alternative. In order to find the alternativemethods for control the cockroach, it will conduct an experiments using impregnated paint woodby insecticide.In this research, insecticide paint wood using an active materials Lambdacyhalothrin10WP. For the first, it make some solution from insecticide paint in

  15. Midgut Transcriptome of the Cockroach Periplaneta americana and Its Microbiota: Digestion, Detoxification and Oxidative Stress Response.

    Jianhua Zhang

    Full Text Available The cockroach, Periplaneta americana, is an obnoxious and notorious pest of the world, with a strong ability to adapt to a variety of complex environments. However, the molecular mechanism of this adaptability is mostly unknown. In this study, the genes and microbiota composition associated with the adaptation mechanism were studied by analyzing the transcriptome and 16S rDNA pyrosequencing of the P. americana midgut, respectively. Midgut transcriptome analysis identified 82,905 unigenes, among which 64 genes putatively involved in digestion (11 genes, detoxification (37 genes and oxidative stress response (16 genes were found. Evaluation of gene expression following treatment with cycloxaprid further revealed that the selected genes (CYP6J1, CYP4C1, CYP6K1, Delta GST, alpha-amylase, beta-glucosidase and aminopeptidase were upregulated at least 2.0-fold at the transcriptional level, and four genes were upregulated more than 10.0-fold. An interesting finding was that three digestive enzymes positively responded to cycloxaprid application. Tissue expression profiles further showed that most of the selected genes were midgut-biased, with the exception of CYP6K1. The midgut microbiota composition was obtained via 16S rDNA pyrosequencing and was found to be mainly dominated by organisms from the Firmicutes phylum, among which Clostridiales, Lactobacillales and Burkholderiales were the main orders which might assist the host in the food digestion or detoxification of noxious compounds. The preponderant species, Clostridium cellulovorans, was previously reported to degrade lignocellulose efficiently in insects. The abundance of genes involved in digestion, detoxification and response to oxidative stress, and the diversity of microbiota in the midgut might provide P. americana high capacity to adapt to complex environments.

  16. Visual pigments, oil droplets, lens, and cornea characterization in the whooping crane (Grus americana)

    Porter, Megan L.; Kingston, Alexandra C. N.; McCready, Robert; Cameron, Evan G.; Hofmann, Christopher M.; Suarez, Lauren; Olsen, Glenn H.; Cronin, Thomas W.; Robinson, Phyllis R.

    2014-01-01

    Vision has been investigated in many species of birds, but few studies have considered the visual systems of large birds and the particular implications of large eyes and long-life spans on visual system capabilities. To address these issues we investigated the visual system of the whooping crane, Grus americana (Gruiformes: Gruidae). G. americana (an endangered species) is one of only two North American crane species and represents a large, long-lived bird where ultraviolet sensitivity may be degraded by chromatic aberrations and entrance of ultraviolet light into the eye could be detrimental to retinal tissues. To investigate the whooping crane visual system we used microspectrophotometry to determine the absorbance spectra of retinal oil droplets and to investigate if the ocular media (i.e., the lens and cornea) absorbs UV light. In vitro expression and reconstitution was used to determine the absorbance spectra of rod and cone visual pigments. The rod visual pigments had wavelengths of peak absorbance (λmax) at 500 nm, while the cone visual pigments λmax values were determined to be 404 nm (SWS1), 450 nm (SWS2), 499 nm (RH2), and 561 nm (LWS), similar to other characterized bird visual pigment absorbance values. The oil droplet cutoff wavelength (λcut) values similarly fell within ranges recorded from other avian species: 576 nm (R-type), 522 nm (Y-type), 506 nm (P-type), and 448 nm (C-type). We confirm that G. americana has a violet-sensitive visual system, although based on the λmax of the SWS1 visual pigment (404 nm) may also have some ability for UV sensitivity.

  17. Literatura marginal americana: do épico confessional ao niilismo erótico

    Lainister de Oliveira Esteves

    2011-08-01

    Full Text Available O objetivo deste artigo é analisar como a prosa ficcional norte americana do século XX se define a partir de um modelo de herói marginal. Autores como Henry Miller; Jack Kerouac e Charles Bukowski dramatizam um tipo de heroísmo baseado na representação de temas como liberdade e redenção que permite pensar como determinados textos se encontram na configuração de uma suposta linhagem literária.

  18. Pollen, Tapetum, and Orbicule Development in Colletia paradoxa and Discaria americana (Rhamnaceae

    M. Gotelli

    2012-01-01

    Full Text Available Tapetum, orbicule, and pollen grain ontogeny in Colletia paradoxa and Discaria americana were studied with transmission electron microscopy (TEM. The ultrastructural changes observed during the different stages of development in the tapetal cells and related to orbicule and pollen grain formation are described. The proorbicules have the appearance of lipid globule, and their formation is related to the endoplasmic reticulum of rough type (ERr. This is the first report on the presence of orbicules in the family Rhamnaceae. Pollen grains are shed at the bicellular stage.

  19. Effects of ripening on rheological properties of avocado pulp (Persea americana mill. Cv. Hass)

    Osorio, F.; Roman, A.; Ortiz, J.

    2015-04-01

    Avocado (Persea americana Mill) Hass variety is the most planted in Chile with a greater trade prospect. The aim of this study was to investigate the effect of maturity on rheological properties of Chilean Avocado Hass pulp. Fresh unripe avocados were washed and peeled, cut and stored at 3 different times; a portion was treated at 5°C and the other was treated at 20°C until it reached 2 lb puncture pressure. During maturation changes would develop due to temperature and time, with internal cellular structure changes. Preliminary results of the rheological characteristics of avocado puree show a Bingham plastic behavior.

  20. Synthesis and configuration of the nonadecenetriol isolated from seeds of Persea americana.

    Yan, Xin; Zhang, Shao-Min; Wu, Yikang; Gao, Po

    2011-10-07

    In an effort to establish the relative as well as absolute configuration of the trypanocidally active natural nonadec-6-en-1,2,4-triol isolated from Persea americana, the (2S,4R), (2S,4S), and (2R,4R) isomers were synthesized. The stereogenic centers taken from enantiopure chiral epoxy building blocks derived from inexpensive and readily available D-glucolactone. The (2R,4R) isomer gave (1)H and (13)C NMR as well as specific rotation in excellent consistence with those reported for the natural triol.

  1. Effects of simulated acid rain on leaf anatomy and micromorphology of Genipa americana L. (Rubiaceae

    Bruno Francisco Sant'Anna-Santos

    2006-03-01

    Full Text Available Experiments were conducted in order to characterize the injuries on leaf structure and micromorphology of G. americana and evaluate the degree of susceptibility of this species to simulated acid rain. Plants were exposed to acid rain (pH 3.0 for ten consecutive days. Control plants were submitted only to distilled water (pH 6.0. Leaf tissue was sampled and fixed for light and scanning electron microscopy. Necrotic interveinal spots on the leaf blade occurred. Epidermis and mesophyll cells collapse, hypertrophy of spongy parenchyma cells, accumulation of phenolic compounds and starch grains were observed in leaves exposed to acid rain. The micromorphological analysis showed, in necrotic areas, plasmolized guard cells and cuticle rupture. Epidermal and mesophyll cells alterations occurred before symptoms were visualized in the leaves. These results showed the importance of anatomical data for precocious diagnosis injury and to determine the sensitivity of G. americana to acid rain.Experimentos foram conduzidos para avaliar o grau de susceptibilidade e determinar as injúrias causadas pela chuva ácida simulada na anatomia e micromorfologia foliar de Genipa americana. Plantas foram expostas à chuva com pH 3,0 durante 10 dias consecutivos. No tratamento controle utilizou-se apenas água destilada (pH 6,0. Amostras foliares foram coletadas e fixadas para microscopia de luz e eletrônica de varredura. Foram observados nas folhas expostas à chuva ácida: necroses pontuais intervenais, colapso das células do mesofilo e da epiderme; hipertrofia do parênquima lacunoso e acúmulo de compostos fenólicos e grãos de amido. A análise micromorfológica evidenciou, nas áreas necrosadas, plasmólise das células-guarda e ruptura da cutícula e da crista estomática. Alterações anatômicas ocorreram antes que sintomas visuais fossem observados nas folhas. Estes resultados comprovam a importância de dados anatômicos na diagnose precoce da injúria e na

  2. Mycotoxin-induced disease in captive whooping cranes (Grus americana) and sandhill cranes (Grus canadensis)

    Olsen, G.H.; Carpenter, J.W.; Gee, G.F.; Thomas, N.J.; Dein, F.J.

    1995-01-01

    In 1987, an epizootic in cranes at the Patuxent Wildlife Research Center, Laurel, Maryland, USA, caused illness in 80% of 300 captive whooping cranes (Grus americana) and sandhill cranes (Grus canadensis) and death of 15 of these cranes. Gross pathology findings were inconclusive and consisted of dehydration, atrophy of fat, renal insufficiency, and small spleens. Extensive testing resulted in isolation of Fusarium sp. mold from constituents of the grain-based diet. Low levels of two mycotoxins, T2 (1-2 ppm) and deoxynivalenol (0.4 ppm), were isolated from the pelleted feed.

  3. Chemical composition and fatty acid profile of rhea (Rhea americana) meat

    Romanelli,Pedro Fernando; Trabuco, Elizeu [UNESP; Scriboni, Andreia Borges; Visentainer, Jesui Vergilio; Souza,Nilson Evelazio de

    2008-01-01

    The purpose of this work was to determine the proximate composition and fatty acid profiles of the Gastrocnemius pars interna intramuscular fat (IMF) of rhea (Rhea americana) thighs. The birds were bred in captivity, fed with balanced feed (Nutriavestruz Crescimento - Purina) and kept in a pen with grass ad lib. The birds of both sexes used in the research weighed 23 kg on average and were aged about twelve (12) months old. They were subjected to hydric diet (12 h) before slaughtering by elec...

  4. O REMEMORAR NA NARRATIVA IBERO-AMERICANA CONTEMPORÂNEA: LITERATURA E CINEMA

    Diogo, Rita de Cássia Miranda; Universidade do Estado do Rio de Janeiro

    2013-01-01

    No presente trabalho nos propomos a analisar a narrativaibero-americana contemporânea em suas versões literária e cinematográfica apartir da articulação dos conceitos benjaminianos em torno da noção de “perdada tradição”. Entendemos o pensamento de Walter Benjamin como constelações,cujos diferentes conceitos ao mesmo tempo que gravitam em torno da ideia de“perda da tradição”, remetem-se mutuamente. Como corpus literário, selecionamos uma obra do escritor espanhol JulioLlamazares (1997), A chu...

  5. Observation on the host parasitoid interaction between Periplaneta americana, the American cockroach and Tetrastichus hagenowii, an oothecal parasitoid

    R. Srinivasan

    1994-09-01

    Full Text Available Investigations were carried out on the host parasitoid interaction between Periplaneta americana, the American cockroach and Tetrastichus hagenowii, an oothecal parasitoid. This gregarious female parasitoid infected and or oviposited in only one host and caused 100 por cento mortality of the infected host. However, increase in parasitoid density decreased the progeny production and influenced the sex ratio. The progenies produced were male biased. When host preference was tested by offering oothecae of different species of cockroaches, T. hagenowii showed a predilection towards the oothecae of P. americana, suggestings its host specificity.

  6. Observation on the host parasitoid interaction between Periplaneta americana, the American cockroach and Tetrastichus hagenowii, an oothecal parasitoid.

    Srinivasan, R; Panicker, K N

    1994-01-01

    Investigations were carried out on the host parasitoid interaction between Periplaneta americana, the American cockroach and Tetrastichus hagenowii, an oothecal parasitoid. This gregarious female parasitoid infected and or oviposited in only one host and caused 100% mortality of the infected host. However, increase in parasitoid density decreased the progeny production and influenced the sex ratio. The progenies produced were male biased. When host preference was tested by offering oothecae of different species of cockroaches. T. hagenowii showed a predilection towards the oothecae of P. americana, suggesting its host specificity.

  7. Effect of certain entomopathogenic fungi on oxidative stress and mortality of Periplaneta americana.

    Chaurasia, Abhilasha; Lone, Yaqoob; Wani, Owais; Gupta, U S

    2016-02-01

    The present paper reports the effects of Metarhizium anisopliae, Isaria fumosoroseus and Hirsutella thompsonaii on Periplaneta americana. I. fumosoroseus and H. thompsonaii were cultured at 28±1°C on potato carrot agar and M. anisopliae was cultured at 28±1°C on potato dextrose agar for 14days. Conidial suspensions of fungi were given to cockroaches through different routes. M. anisopliae shows high virulence against adult cockroaches and mortality ranges from 38.65% to 78.36% after 48h. I. fumosoroseus and H. thompsonii show less virulence compared to M. anisopliae. We also investigated the effect of these three fungi on the activity of lactate dehydrogenase, lipid peroxidation and catalase in different tissues of the insect to gain an understanding of the different target site. The result suggested that the activity of lactate dehydrogenase, catalase and level of malondialdehyde varies in different organs and through different routes of exposure. Based on mortality percentages, all tested fungi had high potentials for biocontrol agents against P. americana. Our study reveals for the first time that I. fumosoroseus and H. thompsonaii fungal infections initiate oxidative stress in the midgut, fat body, whole body and hemolymph of cockroach thereby suggesting them to be the target organs for oxidative damage.

  8. Effect of Agave americana and Agave salmiana Ripeness on Saponin Content from Aguamiel (Agave Sap).

    Leal-Díaz, Ana María; Santos-Zea, Liliana; Martínez-Escobedo, Hilda Cecilia; Guajardo-Flores, Daniel; Gutiérrez-Uribe, Janet Alejandra; Serna-Saldivar, Sergio Othón

    2015-04-22

    Steroidal saponins have shown beneficial health effects. Agave spp. leaves and rhizomes are sources of these compounds, but their presence has not been reported in the aguamiel. Aguamiel is the sweet edible sap from mature agave, and its quality is influenced by the plant ripening stage. The purpose of this research was to identify and quantitate saponins in aguamiel from Agave americana and Agave salmiana at two ripening stages. Saponins and sapogenins were identified with HPLC/ESI-MS/TOF and quantitated with HPLC/ELSD. Results proved the presence of saponins derived from kammogenin, manogenin, gentrogenin, and hecogenin. The saponin content in aguamiel from immature A. salmiana was 2-fold higher (478.3 protodioscin equivalents (PE) μg/g aguamiel (DM)) compared with A. americana (179.0 PE μg/g aguamiel (DM)). In both species, saponin content decreased when plants reached sexual maturity. This should be considered before evaluating the effects of Agave spp. as a source of bioactive saponins.

  9. De Novo Transcriptome Analysis and Detection of Antimicrobial Peptides of the American Cockroach Periplaneta americana (Linnaeus.

    In-Woo Kim

    Full Text Available Cockroaches are surrogate hosts for microbes that cause many human diseases. In spite of their generally destructive nature, cockroaches have recently been found to harbor potentially beneficial and medically useful substances such as drugs and allergens. However, genomic information for the American cockroach (Periplaneta americana is currently unavailable; therefore, transcriptome and gene expression profiling is needed as an important resource to better understand the fundamental biological mechanisms of this species, which would be particularly useful for the selection of novel antimicrobial peptides. Thus, we performed de novo transcriptome analysis of P. americana that were or were not immunized with Escherichia coli. Using an Illumina HiSeq sequencer, we generated a total of 9.5 Gb of sequences, which were assembled into 85,984 contigs and functionally annotated using Basic Local Alignment Search Tool (BLAST, Gene Ontology (GO, and Kyoto Encyclopedia of Genes and Genomes (KEGG database terms. Finally, using an in silico antimicrobial peptide prediction method, 86 antimicrobial peptide candidates were predicted from the transcriptome, and 21 of these peptides were experimentally validated for their antimicrobial activity against yeast and gram positive and -negative bacteria by a radial diffusion assay. Notably, 11 peptides showed strong antimicrobial activities against these organisms and displayed little or no cytotoxic effects in the hemolysis and cell viability assay. This work provides prerequisite baseline data for the identification and development of novel antimicrobial peptides, which is expected to provide a better understanding of the phenomenon of innate immunity in similar species.

  10. Toxicogenetic study of Persea americana fruit pulp oil and its effect on genomic instability.

    Nicolella, Heloiza Diniz; Neto, Francisco Rinaldi; Corrêa, Mariana Beltrame; Lopes, Danillo Henrique; Rondon, Edilaura Nunes; Dos Santos, Luiz Felipe Ribeiro; de Oliveira, Pollyanna Francielli; Damasceno, Jaqueline Lopes; Acésio, Nathália Oliveira; Turatti, Izabel Cristina Casanova; Tozatti, Marcos Gomide; Cunha, Wilson Roberto; Furtado, Ricardo Andrade; Tavares, Denise Crispim

    2017-03-01

    Persea americana Mill., commonly known as avocado, is a tree native to Central America that is widely used as a food source and for the treatment of diseases. This plant has various biological properties such as analgesic, anti-inflammatory and total cholesterol-lowering activity. In view of its pharmacological potential, we conducted a toxicogenetic study of the fruit pulp oil of P. americana (PAO) and investigated its influence on genotoxicity induced by methyl methanesulfonate (MMS) and doxorubicin. V79 cells and Swiss mice were used for the assays. The results showed no genotoxic effects of PAO in the in vitro or in vivo test systems. However, the highest PAO dose tested led to an increase in the levels of aspartate aminotransferase, indicating hepatic/tissue damage. This effect may be related to high concentrations of palmitic acid, the main component of PAO. Furthermore, PAO was effective in reducing the chromosome damage induced by MMS and doxorubicin. These results contribute to the safety assessment of PAO as a medicinal plant for human use.

  11. Fatty acid compositions of seed oils of Haematostaphis barteri and Ximenia americana.

    Eromosele, C O; Eromosele, I C

    2002-05-01

    The fatty acid compositions of the seed oils of Haematostaphis barteri (blood plum) and Ximenia americana (Wild olive) plants were determined by the Gas Chromatography Mass Spectroscopy (GC-MS) technique. H. barteri contained six fatty acids with oleic (69.35%) and stearic (15.40%) the most abundant unsaturated and saturated fatty acids, respectively. Unsaturated higher fatty acids, namely Eicosadienoic (6.92%) and Erucic acid (2.74%) were detected and the total unsaturation for the oil was 79.01%. For X. americana, 10 fatty acids were identified of which seven were unsaturated yielding a total unsaturation of 92.42%. The oil contained essential fatty acids that is, Linoleic (1.34%), Linolenic (10.31%), Arachidonic (0.60%) and varying levels of unsaturated higher fatty acids, namely, Eicosatrienoic (3.39%), Erucic (3.46%) and Nervonic (1.23%) acids. The level of Oleic acid (72.09%) in the oil was close to the value for H. barteri.

  12. Characterization of an Invertebrate-Type Dopamine Receptor of the American Cockroach, Periplaneta americana

    Britta Troppmann

    2014-01-01

    Full Text Available We have isolated a cDNA coding for a putative invertebrate-type dopamine receptor (Peadop2 from P. americana brain by using a PCR-based strategy. The mRNA is present in samples from brain and salivary glands. We analyzed the distribution of the PeaDOP2 receptor protein with specific affinity-purified polyclonal antibodies. On Western blots, PeaDOP2 was detected in protein samples from brain, subesophageal ganglion, thoracic ganglia, and salivary glands. In immunocytochemical experiments, we detected PeaDOP2 in neurons with their somata being located at the anterior edge of the medulla bilaterally innervating the optic lobes and projecting to the ventro-lateral protocerebrum. In order to determine the functional and pharmacological properties of the cloned receptor, we generated a cell line constitutively expressing PeaDOP2. Activation of PeaDOP2-expressing cells with dopamine induced an increase in intracellular cAMP. In contrast, a C-terminally truncated splice variant of this receptor did not exhibit any functional property by itself. The molecular and pharmacological characterization of the first dopamine receptor from P. americana provides the basis for forthcoming studies focusing on the significance of the dopaminergic system in cockroach behavior and physiology.

  13. Anti-inflammatory activity of aqueous extracts and steroidal sapogenins of Agave americana.

    Peana, A T; Moretti, M D; Manconi, V; Desole, G; Pippia, P

    1997-06-01

    Lyophilized aqueous extracts obtained from Agave americana L (Agavaceae) collected in the north of Sardinia were characterized with regard to their steroidal sapogenin content. Extracts of A. americana and genins isolated from them were evaluated for anti-inflammatory properties by testing their effects on carrageenin-induced edema. The effect of orally administered genins on gastric mucous membranes was also assessed. Lyophilized extracts administered by the intraperitoneal route at doses equivalent to 200 and 300 mg/kg of fresh plant starting material, showed good anti-inflammatory activity. Doses of genins (total steroidal sapogenins, hecogenin and tigogenin) equivalent to the amount in the lyophilized extracts produced an antiedentatous effect which was much stronger and more efficacious than that obtained with an i.p. administration of 5 mg/kg of indomethacin or dexamethasone 21-phosphate at a dose equivalent to the molar content of hecogenin administered. At the doses used to evaluate the anti-inflammatory activity, the genins did not have any harmful effect on the gastric mucous membranes. Lesions occurred when significantly higher doses of hecogenin were given, but gastric damage was still less than that caused by the drugs used for comparative purposes.

  14. The Complex of Scolytinae Inhabiting Persea borbonia and Persea americana in Florida: Possible Interactions with Other Species

    A diverse number of Scolytinae (Coleoptera: Curculionidae) have been found inhabiting Persea borbonia and P. americana in Florida during 2009 and 2010. They include the exotic redbay ambrosia beetle, Xyleborus glabratus, vector of Raffaelea lauricola, X. volvulus, X. ferrugineus, Xylosandrus crassiu...

  15. Fusarium symbionts of an ambrosia beetle (Euwallacea sp.) in southern Florida are pathogens of avocado, Persea americana

    Fusarium dieback, a destructive disease of avocado (Persea americana), was reported in California and Israel in 2012. It is associated with an ambrosia beetle, Euwallacea sp., and damage caused by an unnamed symbiont of the beetle in Clade 3 of the Fusarium solani species complex (FSSC) designated p...

  16. A Comunidade Sul-Americana de Nações no contexto da política externa do Brasil

    Carlos Ribeiro Santana

    2011-02-01

    Full Text Available O presente artigo tem como finalidade realizar um balanço sobre o significado da I Reunião de Chefes de Estado da Comunidade Sul-Americana de Nações, no que se refere à política externa.

  17. Seed rescue from photoperiod sensitive American Joint Vetch (Aeschynomene americana L.) accessions using hydroponic cloning and aeroponics

    American joint vetch, Aeschynomene Americana L. is a self-pollinated tropical pasture legume and the USDA, ARS, PGRCU curates 137 accessions from the United States, S. America, Mexico, Central America, and Zambia. Many accessions in this collection are photoperiod sensitive due to their typical flow...

  18. Reunión y escritura femeninas en los contextos de las independencias americanas

    Patricia Martínez i Álvarez

    2010-10-01

    Full Text Available En los ámbitos de las relaciones de género y la representación de la diferencia sexual femenina, las mujeres –en el contexto de los procesos de emancipación y de construcción de los nuevos Estados en América Latina en el siglo XIX– llevaron a cabo proyectos propios de significación, al margen de los proyectos políticos que había en el escenario. El objetivo de la investigación que sustenta el artículo, es dar lectura, a la luz de los sentidos que las mujeres les dieron, a textos y contextos de aquel momento. Se recurre a fragmentos de dos de las obras literarias más importantes de finales del siglo XIX, para afirmar una historia de las mujeres que invita al final de la colonialidad americana.

  19. La epidemiología, una propuesta para explicar la despoblación americana

    Malvido, Elsa

    2003-04-01

    Full Text Available This paper discusses American depopulation during the 16th century in the light of modern epidemiology, concluding that contagious diseases introduced in New Spain by the Spanish Conquest reduced in many ways its native inhabitants as much as 90 per cent, as Borah and Cook’s studies claimed.

    El asunto sobre la despoblación americana se discute a través de elementos tomados de la epidemiología moderna, y sin ningún interés antieuropeo, se puede concluir que debido a las pandemias, epidemias y endemias, resultado de la conquista Castellana, la población de la Nueva España se redujo en un siglo hasta un 90%, como lo habían propuesto Borah y Cook en otros estudios.

  20. Impact of deltamethrin on cockroaches (Periplaneta americana and its residue on environment

    Endang Puji Astuti

    2015-01-01

    Full Text Available AbstrakLatar belakang: Penggunaan insektisida secara intensif tidak hanya memberikan dampak pada spesies target, namun juga pada spesies non-target dan lingkungan. Pada penelitian ini dilakukan penilaian pengaruh residu deltametrin terhadap kecoa (Periplanetaamericana, ikan lele (Clarias batrachus, dan nila (Oreochromis niloticus. Selain itu laju infiltrasi residu pada tipe tanah dengan komposisi tanah yang berbeda juga diukur. Metode: Percobaan dilakukan terhadap kecoa yang dikembangbiakan  di laboratorium di Institut Pertanian Bogor. Perlakuan dengan tiga ulangan dan lima taraf konsentrasi deltamethrin: 0.8%, 0.4%, 0.2%, 0.1% and 0.05% (v/v dilakukan untuk mengukur pengaruh residu terhadap kecoa selama 24 dan 48 jam. Penentuan LC  dilakukan dengan analisis Probit. Konsentrasi letal yang didapatkan kemudiandiujikan kepada ikan lele dan nila dengan metode semprot. Untuk perbandingan, uji efek organofosfat dengan konsentrasi 1 ppm dan 10 ppm dilakukan melalui metode soaking kepada dua jenis ikan tersebut. Laju infiltrasi residu pada tiga tipe komposisi tanah diukur menggunakan lysimeter. Hasil: Hasil penelitian menunjukkan bahwa LC50 residu deltamethrin terhadap kecoa tercapai pada konsentrasi 0,2% pada 24-jam perlakuan. Lima puluh persen ikan nila mati pada perlakuan deltamethrin0,2% selama 24 jam. Laju infiltrasi residu lebih tinggi pada jenis tanah berpasir (5 ml/menit dibandingkan jenis tanah yang didominasi tanah liat. Kesimpulan: Selain memiliki efek mematikan pada kecoa, Deltamethrin 0,2% mencemari tanah dan air, serta membunuh ikan nila (O. niloticus. (Health Science Indones 2014;2:94-9Kata kunci: deltamethrin, efek residu, P. americana, O. niloticus, tanahAbstractBackground: Intensive use of chemical insecticides not only affect the targetspecies, but also non-target species and environment. In this study, we examined residual effect of deltamethrin on cockroaches (Periplaneta americana, catfishes (Clarias batrachus and nile

  1. Two glucosylated abscisic acid derivates from avocado seeds (Persea americana Mill. Lauraceae cv. Hass).

    del Refugio Ramos, María; Jerz, Gerold; Villanueva, Socorro; López-Dellamary, Fernando; Waibel, Reiner; Winterhalter, Peter

    2004-04-01

    Phytochemical investigation of avocado seed material (Persea americana Mill., Lauraceae) resulted in the isolation of two glucosylated abscisic acid derivates. One of these was not known as a natural product and can be regarded as a potential 'missing link' in abscisic acid metabolism in plants. After fractionation by high-speed countercurrent chromatography, and multiple steps of column chromatography, structures were elucidated by 1D-, 2D-NMR, electrospray-MS to be the novel beta-d-glucoside of (1'S,6'R)-8'-hydroxyabscisic acid, and (1'R,3'R,5'R,8'S)-epi-dihydrophaseic acid beta-d-glucoside. Absolute configuration was determined by circulardichroism, optical rotation, and by NOE experiments.

  2. Structural analysis of fructans from Agave americana grown in South Africa for spirit production.

    Ravenscroft, Neil; Cescutti, Paola; Hearshaw, Meredith A; Ramsout, Ronica; Rizzo, Roberto; Timme, Elizabeth M

    2009-05-27

    Fructans isolated from Agave americana grown in South Africa are currently used for spirit production. Structural studies on water-soluble fructans were performed to facilitate the development of other applications including its use as a prebiotic. Acid hydrolysis followed by HPAEC-PAD analysis confirmed that the fructan was composed of glucose and fructose, and size analysis by HPAEC-PAD and size exclusion chromatography indicated that the saccharides have a DP range from 6 to 50. An average DP of 14 was estimated by (1)H NMR analysis. Linkage analysis and ESI-MS studies suggest that A. americana has a neofructan structure consisting of a central sucrose to which (2 → 1)- and (2 → 6)-linked β-D-Fruf chains are attached. The (2 → 1)-linked units extend from C-1 of Fru and C-6 of glucose, whereas the (2 → 6)-linked β-D-Fruf units are attached to C-6 of the central Fru. This structure accounts for the presence of equimolar amounts of 1,6-linked Glu and 1,2,6-linked Fru found in linkage analysis and the multiplicity of the NMR signals observed. Detailed ESI-MS studies were performed on fructan fractions: native, periodate oxidized/reduced, and permethylated oligomers. These derivatizations introduced mass differences between Glc and Fru following oxidation and between 1,2-, 1,6-, 2,6-, and 1,2,6-linked units after methylation. Thus, ESI-MS showed the presence of a single Glc per fructan chain and that it is predominantly internal, rather than terminal as found in inulin. These structural features were confirmed by the use of 1D and 2D NMR experiments.

  3. Monitoramento de fitoplâncton e microcistina no reservatório da UHE Americana Monitoring phytoplankton and microcistyn at the Americana reservoir

    R.A.R. Ferreira

    2005-06-01

    Full Text Available Este trabalho foi realizado na UHE Americana, pertencente à Companhia Paulista de Força e Luz, e faz parte de um projeto de pesquisa e desenvolvimento realizado em conjunto com a Faculdade de Ciências Agronômicas - UNESP, de Botucatu. As amostragens de água foram realizadas nos meses de fevereiro, abril, junho e outubro de 2004. As características analisadas foram: temperatura da água, pH, oxigênio dissolvido, condutividade, nitrogênio total, nitrito, nitrato, amônia, fósforo total, fosfato, fosfato inorgânico, juntamente com análise qualitativa e quantitativa da comunidade fitoplanctônica e a toxicidade. O reservatório apresentou valores elevados de fósforo total, variando de 18 a 509 µg L-1; fosfato, de 4 a 463 µg L-1; nitrogênio total, de 0,99 a 17,25 mg L-1; e nitrato, de 0,26 a 15,29 mg L-1. Para a comunidade fitoplanctônica foram encontrados 103 táxons em todo o período amostrado; a maior riqueza foi encontrada no ponto P06, e a maior pobreza de táxons, nos pontos localizadas no corpo central do reservatório (P02, P03, P04 e P05. A maior concentração de cianofícea ocorreu em abril de 2004: 5.375.175 ind. L-1. As espécies que apresentaram as maiores densidades foram Microcystis aeruginosa, Anabaena spiroides, Microcystis sp. e Pseudoanabaena mucicola; a maior densidade foi apresentada por Anabaena spiroides, com 4.178.084 ind. L-1. Nos meses de junho e outubro a classe Cryptophyceae teve uma grande contribuição para a densidade total. Apesar da grande densidade de cianobactérias, os valores de toxicidade ficaram abaixo do limite permitido pela Portaria nº 1.469.This work was carried out at the Americana Reservoir, owned by Companhia Paulista de Força e Luz, and was part of a joint R&D project with Faculdade de Ciências Agronômicas - Botucatu - São Paulo - Brazil. Water sampling was collected in February, April, June and October 2004. The following characteristics were analyzed: water temperature, p

  4. Estudo de estabilidade sob armazenamento da carne de ema (Rhea americana Study of the great rhea (Rhea americana meat stability under storage

    Amanda V. Pereira

    2006-06-01

    Full Text Available Utilizou-se o músculo Iliotibialis lateralis da sobrecoxa da ema (Rhea americana para a realização das análises de estabilidade da carne sob armazenamento em refrigeração e congelamento. Na avaliação da carga microbiológica da carne foram realizadas análises de Coliformes Totais, Coliformes Fecais (Escherichia coli e psicrotróficos. No período de zero a 12 dias de refrigeração determinou-se o pH da carne e a degradação protéica através da análise de Nitrogênio Volátil Total (NVT. Durante o congelamento (período de 60 dias avaliou-se o pH e a oxidação lipídica pela análise do número de TBA da carne armazenada. As amostras sob refrigeração e congelamento mostraram-se adequadas ao consumo, não apresentando contaminação microbiológica durante todo o período de estocagem. Os valores de Nitrogênio Volátil Total (NVT da carne sob refrigeração tiveram uma evolução insignificante (4% entre o tempo zero e o 12º dia de estocagem. Durante o congelamento ocorreu um aumento significativo do número de TBA nas amostras, com variações de 1,5-2,5 mg de SRATB/1.000 g amostra. Os valores de pH da carne de ema armazenada sob refrigeração e congelamento mantiveram-se praticamente estáveis em torno de 5,8.The Great Rhea (Rhea americana thigh muscle Iliotibialis lateralis was used to perform analyses of the meat stability under refrigeration and freezing storage. Analyses on fecal (Escherichia coli, total coliforms and psycrotrophic organisms were performed for the evaluation of the microbiological load at the meat. The meat pH and protein degradation have been determined by Total Volatile Basic Nitrogen (TVBN analyses from time zero to the 12th day of refrigeration. During the freezing period (60 days, the pH and lipidic oxidation have evaluated through the analyses of TBARs number in the stored meat. The samples under refrigeration and freezing were adequate to consumption and they not presented microbiological

  5. Rendimentos do abate e composição da carne de ema (Rhea americana Slaughtering yield and composition of great rhea meat (Rhea americana

    Amanda Vieira Pereira

    2006-09-01

    Full Text Available O objetivo deste trabalho foi estudar o desempenho zootécnico da ema (Rhea americana, rendimentos do abate, composição química e análise sensorial da carne. Onze animais foram submetidos a um sistema de confinamento, recebendo ração balanceada à vontade e com acesso a um piquete. Antes do abate as emas permaneceram em jejum hídrico por 12 h, foram pesadas e submetidas ao processamento: insensibilização, sangria, depenagem manual, esfola e evisceração. As emas apresentaram peso vivo médio, no momento do abate, de 19,393 kg, desempenho médio de 529,82 de consumo de ração/dia, 53,20 g de ganho de peso/dia e 9,44 de conversão alimentar. Em relação ao peso de carcaça, mostraram rendimento médio de 63,33% de carne, 5,09% de gordura e 22% de ossos. A composição centesimal média da carne de ema foi de 22,81% de proteína, 1,59% de lipídios, 1,62% de cinzas e de 74,72% de umidade. O conteúdo de colágeno variou de 0,94% a 1,12% e o teor de colesterol foi de 65,44 mg por 100 g de carne. Pela análise sensorial, verificou-se que a carne de ema processada foi bem aceita pelos provadores. Assim, admite-se que a carne de ema pode ser consumida como fonte de proteína animal com baixo teor de lipídios.The aim of this project is to study the zootechnical performance of rhea (Rhea americana, including slaughtering yields, chemical composition and sensorial analysis of its meat. Eleven birds were kept under confinement and each had a balanced ration on demand and access to run. Before slaughtering, the rhea were not allowed to drink water for 12 h, they were weighed and underwent the following: stunning, bleeding, manual feather plucking, skinning, and evisceration. The average rhea weight at the time of slaughtering was 19.393 kg, their average performance for food intake 529.82 g ration/day, weight gain of 53.20 g/day, and 9.44 food conversion. Relative to the carcass weight, the average meat yield was 63.33%, whereby 5.09% was fat

  6. Cloning of PaAtg8 and roles of autophagy in adaptation to starvation with respect to the fat body and midgut of the Americana cockroach, Periplaneta americana.

    Park, Moon Soo; Takeda, Makio

    2014-05-01

    Starvation, in particular amino acid deprivation, induces autophagy in trophocytes (adipocytes), the major component of the fat body cell types, in the larvae of Drosophila melanogaster. However, the fat body of cockroach has two additional cell types: urocytes depositing uric acid in urate vacuoles as a nitrogen resource and mycetocytes harboring an endosymbiont, Blattabacterium cuenoti, which can synthesize amino acids from the metabolites of the stored uric acid. These cells might complement the roles of autophagy in recycling amino acids in the fat body or other organs of cockroaches under starvation. We investigate the presence of autophagy in tissues such as the fat body and midgut of the American cockroach, Periplaneta americana, under starvation by immunoblotting with antibody against Atg8, a ubiquitin-like protein required for the formation of autophagosomes and by electron microscopy. Corresponding changes in acid phosphatase activity were also investigated as representing lysosome activity. Starvation increased the level of an autophagic marker, Atg8-II, in both the tissues, extensively stimulating the formation of autophagic compartments in trophocytes of the fat body and columnar cells of the midgut for over 2 weeks. Acid phosphatase showed no significant increase in the fat body of the starved cockroaches but was higher in the midgut of the continuously fed animals. Thus, a distinct autophagic mechanism operates in these tissues under starvation of 2 weeks and longer. The late induction of autophagy implies exhaustion of the stored uric acid in the fat body. High activity of acid phosphatase in the midgut of the fed cockroaches might represent enhanced assimilation and not an autophagy-related function.

  7. Sensorial, biochemical and microbiological evaluations of guacamole, an avocado based product, under cold storage and added with ascorbic acidAvaliações sensoriais, bioquímicas e microbiológicas do guacamole, um produto à base de abacate, sob armazenamento a frio e com adição de ácido ascórbico

    Lídia Raquel de Carvalho

    2011-07-01

    Full Text Available The elaboration of avocado products for commercialization keeping their characteristics of fresh product has been limited. The cut avocado darkens quickly and their sensorial characteristics are modified with the storage. In the present research, the sensorial parameters, microbiological stability, and peroxidase and polyphenoloxidase activity were evaluated in guacamole added with ascorbic acid and conserved under low temperature, by using avocado variety Hass. Products were conditioned in polyethylene+nylon packages with and without vacuum application; then, they were subjected to the slow and fast freezing (-18ºC and stored in freezer (-18ºC. Evaluations were performed at the moment of elaboration of the product (t0 and at 3, 7 and 30 days post-storage. At t30, samples were kept under refrigeration (4 ±1oC and evaluated at 3, 5 and 7 days. After the 30 days of storage, –18 ºC under freezing, followed by thawing and keeping at 4±1ºC for 7 days, the notes for the sensorial parameters decreased. The peroxidase activity was totally inhibited in the elaborated product and the polifenol oxidase activity considerably decreased in the guacamole (20.07 mM catechol/g fresh matter relative to those in the fruit (58.31 mM catechol/g fresh matter, however with no significant variation during storage (at -18ºC. The samples were microbiologically stable under the conditions of the present study. The addition of ascorbic acid contributed to the conservation of the frozen avocado product by decreasing the enzymatic activity. However, the sensorial parameters are prejudiced under thawing and storage at 4 ±1oC.A elaboração de produtos de abacate para comercialização mantendo suas características de produto fresco é limitada, já que o fruto cortado escurece rapidamente e suas características sensoriais são modificadas com o armazenamento. Na presente pesquisa, avaliaram-se os parâmetros sensoriais, estabilidade microbiológica e atividade de

  8. Comparative Growth Inhibitory Assay Of The Methanol Extract Of The Leaf And Seed Of Persea americana Mill (Lauraceae

    Ayinde B.A

    2013-03-01

    Full Text Available Persea americana is one of the recipes used by traditional healers in parts of Edo State of Nigeria for the treatment of cancer. This claim was investigated using predictive bench-top assay method of germinating seeds radicle of guinea corn (Sorghum bicolor. The powdered leaves and seeds were extracted by Soxhlet using methanol. The growth inhibitory effect was examined using radicle lengths of germinating seeds of Sorghum bicolor at 1-30 mg/ml. The methanol extract of the leaf of Persea americana was subjected to solvent partitioning. Phytochemical screening showed the presence of saponins, flavonoids, tannins and cardiac glycosides with no traces of anthracene derivatives. The aqueous fraction was more active than the chloroform fraction and completely inhibited the germination of the seeds at 20mg/ml. The results suggest the probable use of the plant in preparing recipes for tumor-related ailments. However, using appropriate human cell lines will further justify this claim.

  9. Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.

    De La Torre-Ruiz, Neyser; Ruiz-Valdiviezo, Víctor Manuel; Rincón-Molina, Clara Ivette; Rodríguez-Mendiola, Martha; Arias-Castro, Carlos; Gutiérrez-Miceli, Federico Antonio; Palomeque-Dominguez, Héctor; Rincón-Rosales, Reiner

    2016-01-01

    The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (pagave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.

  10. IN VITRO CYTOTOXICITY STUDY OF AGAVE AMERICANA, STRYCHNOS NUX-VOMICA AND ARECA CATECHU EXTRACTS USING MCF-7 CELL LINE

    Chetan C Anajwala; Patel, Rajesh M.; Sanjay L Dakhara; Jitesh K Jariwala

    2010-01-01

    Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water...

  11. Análise institucionalista da integração sul-americana = Institutionalist analysis of South American integration

    Mallmann, Maria Izabel

    2010-01-01

    Full Text Available A integração sul-americana é aqui identificada pelos compromissos regionais assumidos ao longo da década de 1990; lançados durante a 7ª Cúpula do Grupo do Rio, de outubro de 1993, seguidos pelas Cúpulas de presidentes da América do Sul (2000, 2002, 2004, pela criação da Iniciativa para a Integração da Infraestrutura Regional Sul-Americana (IIRSA, em 2000, da Comunidade Sul-Americana das Nações (Casa, em 2005 e da União das Nações Sul-Americanas (Unasul, em 2007. Analisa-se o processo tendo como parâmetro as condições e mecanismos apontados por estudos institucionalistas como essenciais para o sucesso de um processo de integração como, por exemplo, a simetria regional e a estabilidade interna, dentre outras. Constata-se que essas duas são as condições mais problemáticas no caso sul-americano, uma vez que interpõem obstáculos ao avanço nas demais. Um balanço do estudo realizado até aqui permite estimar que o processo de integração tende a ser mais longo e errático do que seria desejável, uma vez que se encontra no que poderia ser entendida como fase inicial de implementação de mecanismos e de geração das condições básicas necessárias

  12. Phylogenomic analysis of Odyssella thessalonicensis fortifies the common origin of Rickettsiales, Pelagibacter ubique and Reclimonas americana mitochondrion.

    Kalliopi Georgiades

    Full Text Available BACKGROUND: The evolution of the Alphaproteobacteria and origin of the mitochondria are topics of considerable debate. Most studies have placed the mitochondria ancestor within the Rickettsiales order. Ten years ago, the bacterium Odyssella thessalonicensis was isolated from Acanthamoeba spp., and the 16S rDNA phylogeny placed it within the Rickettsiales. Recently, the whole genome of O. thessalonicensis has been sequenced, and 16S rDNA phylogeny and more robust and accurate phylogenomic analyses have been performed with 65 highly conserved proteins. METHODOLOGY/PRINCIPAL FINDINGS: The results suggested that the O. thessalonicensis emerged between the Rickettsiales and other Alphaproteobacteria. The mitochondrial proteins of the Reclinomonas americana have been used to locate the phylogenetic position of the mitochondrion ancestor within the Alphaproteobacteria tree. Using the K tree score method, nine mitochondrion-encoded proteins, whose phylogenies were congruent with the Alphaproteobacteria phylogenomic tree, have been selected and concatenated for Bayesian and Maximum Likelihood phylogenies. The Reclinomonas americana mitochondrion is a sister taxon to the free-living bacteria Candidatus Pelagibacter ubique, and together, they form a clade that is deeply rooted in the Rickettsiales clade. CONCLUSIONS/SIGNIFICANCE: The Reclinomonas americana mitochondrion phylogenomic study confirmed that mitochondria emerged deeply in the Rickettsiales clade and that they are closely related to Candidatus Pelagibacter ubique.

  13. IN VITRO CYTOTOXICITY STUDY OF AGAVE AMERICANA, STRYCHNOS NUX-VOMICA AND ARECA CATECHU EXTRACTS USING MCF-7 CELL LINE

    Anajwala Chetan C.

    2010-06-01

    Full Text Available Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water. By trypan blue dye exclusion method, Viability of MCF-7 and Vero cell lines were 85.50 and 81.13%, respectively. IC50 value of methanol extract of Agave americana leaves and aqueous extract of Areca catechu fruits were found to be 545.9 & 826.1 µg/ml by SRB assay and 775.1 & 1461µg/ml by MTT assay, respectively, against MCF-7 cell line. From cytotoxicity study data by SRB and MTT assay, it revealed that methanol extract of Agave americana and aqueous extract of Areca catechu are potent cytotoxic.

  14. Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.

    Neyser De La Torre-Ruiz

    Full Text Available ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA, solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (p < 0.05 on plant growth and the sugar content of A. americana, showing that these native plant growth-promoting bacteria are a practical, simple, and efficient alternative to promote the growth of agave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.

  15. Chemical identification, emission pattern and function of male-specific pheromones released by a rarely swarming locust, Schistocerca americana.

    Stahr, Christiane; Svatoš, Aleš; Seidelmann, Karsten

    2013-01-01

    Pheromones serve key functions in the biology of swarming locusts. However, research has focused largely on the mass-swarming desert locust, Schistocerca gregaria. We extended these investigations to the pheromonal profile of the rarely swarming American bird grasshopper, S. americana (Drury). The headspace of mature gregarious S. americana males contained three characteristic electroantennogram-active components: (Z)-3-nonen-1-ol, (Z)-2-octen-1-ol, and nonanal. These substances were accompanied by aromatics such as phenol that are also released by females and immatures. Male-specific pheromone components were released independently from epidermal gland cells, with the highest emission rate being for (Z)-3-nonen-1-ol from the abdomen and legs. The emission of the major compound, (Z)-3-nonen-1-ol, is stress-sensitive, and coincides with sexual maturity and crowding. The emission pattern strongly supports a role of (Z)-3-nonen-1-ol in the reproductive biology of S. americana. The pheromone is involved in courtship-inhibition and is used as mate assessment pheromone in cryptic female choice. In double mating experiments, females choose sperm of males with high (Z)-3-nonen-1-ol emission. Furthermore the pheromone accelerated maturation of immature adults and supports synchronization of sexual development.

  16. Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars.

    Raymond Chia, Teck Wah; Dykes, Gary A

    2010-07-01

    The epicarp and seed of Persea Americana Mill. var. Hass (Lauraceae), Persea Americana Mill. var. Shepard, and Persea americana Mill. var Fuerte cultivars of mature avocados (n = 3) were ground separately and extracted with both absolute ethanol and distilled water. Extracts were analyzed for antimicrobial activity using the microtiter broth microdilution assay against four Gram-positive bacteria, six Gram-negative bacteria, and one yeast. Antimicrobial activity against two molds was determined by the hole plate method. The ethanol extracts showed antimicrobial activity (104.2-416.7 microg/mL) toward both Gram-positive and Gram-negative bacteria (except Escherichia coli), while inhibition of the water extracts was only observed for Listeria monocytogenes (93.8-375.0 microg/mL) and Staphylococcus epidermidis (354.2 microg/mL). The minimum concentration required to inhibit Zygosaccharomyces bailii was 500 microg/mL for the ethanol extracts, while no inhibition was observed for the water extracts. No inhibition by either ethanol or water extracts was observed against Penicillium spp. and Aspergillus flavus.

  17. Acute Toxicity Investigation and Anti-diarrhoeal Effect of the Chloroform-Methanol Extract of the Leaves of Persea americana.

    Christian E, Odo; Okwesili Fc, Nwodo; Parker E, Joshua; Okechukwu Pc, Ugwu

    2014-01-01

    Persea americana is a plant used by traditional medicine practitioners to treat ailments including diarrhoea and diabetes mellitus in Nigeria. Hence, the chloroform and the methanol fractions of the chloroform-methanol extract of the leaves of P. americana were evaluated for their acute toxicity as well as anti-diarrhoeal effects in Wistar rats to substantiate this claim. The chloroform and methanol fractions [at graded doses of 100 and 200 mg/Kg body weight (b.w) of each] were studied for their anti-diarrhoeal effects in terms of the reductions in the wetness of faeces and the frequency of defaecation of castor oil-induced diarrhoea. To understand the mechanism of their anti-diarrhoeal effects, their actions were further evaluated on castor oil-induced enteropooling (intestinal fluid accumulation). The median lethal dose (LD50) of the methanol fraction was found to be less than 5000 mg/Kg b.w. At the two doses, the chloroform and the methanol fractions showed dose-dependent significant (p americana possesses significant anti-diarrhoeal effect and may be a potent source of anti-diarrhoeal drug(s) in future.

  18. Biochemical and molecular modulation of CCl4-induced peripheral and central damage by Tilia americana var. mexicanaextracts.

    Coballase-Urrutia, Elvia; Cárdenas-Rodríguez, Noemí; González-García, María Carolina; Núñez-Ramírez, Eithan; Floriano-Sánchez, Esaú; González-Trujano, María Eva; Fernández-Rojas, Berenice; Pedraza-Chaverrí, José; Montesinos-Correa, Hortencia; Rivera-Espinosa, Liliana; Sampieri, Aristides Iii; Carmona-Aparicio, Liliana

    2017-03-01

    Around the world, species from the genus Tilia are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous extracts (100 mg/kg, i.p.) obtained from the leaves of Tilia americana var. mexicana on CCl4-induced liver and brain damage in the rat. Protection was observed in the liver and brain (cerebellum, cortex and cerebral hemispheres) by measuring the activity of antioxidant enzymes and levels of malondialdehyde (MDA) using spectrophotometric methods. Biochemical parameters were also assessed in serum samples from the CCl4-treated rats. The T. americana var. mexicana leaf extracts provided significant protection against CCl4-induced peripheral and central damage by increasing the activity of antioxidant enzymes, diminishing lipid peroxidation, and preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-globulin (γ-GLOB), serum albumin (ALB), total bilirubin (BB), creatinine (CREA) and creatine kinase (CK), relative to the control group. Additionally, we correlated gene expression with antioxidant activity in the experimental groups treated with the organic and aqueous Tilia extracts and observed a non-statistically significant positive correlation. Our results provide evidence of the underlying biomedical properties of T. americana var. mexicana that confer its neuro- and hepatoprotective effects.

  19. Biochemical and molecular modulation of CCl4-induced peripheral and central damage by Tilia americana var. mexicana

    Elvia Coballase-Urrutia

    2017-03-01

    Full Text Available Around the world, species from the genus Tilia are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous extracts (100 mg/kg, i.p. obtained from the leaves of Tilia americana var. mexicana on CCl4-induced liver and brain damage in the rat. Protection was observed in the liver and brain (cerebellum, cortex and cerebral hemispheres by measuring the activity of antioxidant enzymes and levels of malondialdehyde (MDA using spectrophotometric methods. Biochemical parameters were also assessed in serum samples from the CCl4-treated rats. The T. americana var. mexicana leaf extracts provided significant protection against CCl4-induced peripheral and central damage by increasing the activity of antioxidant enzymes, diminishing lipid peroxidation, and preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP, γ-globulin (γ-GLOB, serum albumin (ALB, total bilirubin (BB, creatinine (CREA and creatine kinase (CK, relative to the control group. Additionally, we correlated gene expression with antioxidant activity in the experimental groups treated with the organic and aqueous Tilia extracts and observed a non-statistically significant positive correlation. Our results provide evidence of the underlying biomedical properties of T. americana var. mexicana that confer its neuro- and hepatoprotective effects.

  20. Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana.

    Chen, Yan; He, Ming; Li, Zhao-Qun; Zhang, Ya-Nan; He, Peng

    2016-06-09

    Periplaneta americana is a notorious urban pest prevalent in human habitats; very little is known about its chemosensory mechanism. Employing the advanced next-generation sequencing technique, in the present study, we conducted transcriptome sequencing and analysis of the antennae of the adult males and females as well as their mouthparts using an Illumina platform. This resulted in the discovery of a huge number of the members of all major known chemosensory receptor families in P. americana, including 96 odorant receptors (ORs), 53 ionotropic receptors (IRs), and 33 gustatory receptors (GRs). Tissue expression profiles showed most of them mainly expressed in antennae and phylogenetic analysis demonstrated the expansion in the clade distinguishing them from other functionally well-known Lepidoptera species. A high percentage of chemosensory receptor genes (ORs in particular) showing female antenna bias in mRNA expression was observed. Our results provide a basis for further investigations on how P. americana coordinates its chemosensory receptor genes in chemical communication with environments, and for development of novel pest management approaches.

  1. In vitro cytotoxicity study of agave americana, strychnos nuxvomica and areca catechu extracts using mcf-7 cell line.

    Anajwala, Chetan C; Patel, Rajesh M; Dakhara, Sanjay L; Jariwala, Jitesh K

    2010-04-01

    Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nuxvomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water. By trypan blue dye exclusion method, Viability of MCF-7 and Vero cell lines were 85.50 and 81.13%, respectively. IC(50) value of methanol extract of Agave americana leaves and aqueous extract of Areca catechu fruits were found to be 545.9 & 826.1 μg/ml by SRB assay and 775.1 & 1461pg/ml by MTT assay, respectively, against MCF-7 cell line. From cytotoxicity study data by SRB and MTT assay, it revealed that methanol extract of Agave americana and aqueous extract of Areca catechu are potent cytotoxic.

  2. In vitro cytotoxicity study of agave Americana, strychnos Nuxvomica and Areca catechu extracts using MCF-7 cell line

    Chetan C Anajwala

    2010-01-01

    Full Text Available Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water. By trypan blue dye exclusion method, Viability of MCF-7 and Vero cell lines were 85.50 and 81.13%, respectively. IC 50 value of methanol extract of Agave americana leaves and aqueous extract of Afeca catechu fruits were found to be 545.9 & 826.1 μg/ml by SRB assay and 775.1 & 1461pg/ml by MTT assay, respectively, against MCF-7 cell line. From cytotoxicity study data by SRB and MTT assay, it revealed that methanol extract of Agave americana and aqueous extract of Areca catechu are potent cytotoxic.

  3. La responsabilidad social en la Corporación Universitaria Americana

    Nohora Barrios Castro

    2009-07-01

    Full Text Available ResumenLa propuesta de este ensayo es interpretar la forma como se desenvuelve la educación en la Corporación UniversitariaAmericana, donde se espera que los futuros profesionales actúen con responsabilidad social, estrategia queconsidero indispensable tanto para el incremento del capital social como del desarrollo sostenible donde finalmentese logrará una disminución en los altos índices de pobreza actuales. Acelerar el crecimiento y el desarrollo social,en concreto, se logra desarrollando acciones e interactuando en y con los grupos, y nos referimos al capital socialdonde las redes sociales, las organizaciones, los valores compartidos y la capacidad de actuar sinérgicamenteconstituye el escenario donde se encuentra la Corporación Universitaria Americana donde lo más importante delproceso es que se aprenda a actuar en forma socialmente responsable y ética, esto es cuando se instituyen unconjunto de prácticas obligatorias y voluntarias orientadas a promover la satisfacción de las necesidades socialesde sus integrantes y las de los miembros de su comunidad.Palabras Claves:Responsabilidad Social. Desarrollo Social. Capital Humano.Abstract.The proposal of this essay is to interpret the way in which the education at the American Universitarian Corporationis unfolding, where it is expected that the future professionals perform with social responsibility; strategy thatI consider indispensable as much to the increment of the social capital as to the sustainable development wherefinally will be attain a reduction in the high indexes of present poverty. To acelerate the growth and the social development,in concrete, is attain developing actions and interacting, in and with the groups, and we are refering to thesocial capital where the social networks, the organizations, the shared values, and the capacity to act synergicallyconstitutes the stage where is find the American Universitarian Corporation. Where the most important part of theprocess

  4. Um olhar sistêmico sobre a crise norte-americana

    CHRISTIAN CARVALHO GANZERT

    Full Text Available RESUMO No contexto contemporâneo, a economia global pode ser descrita como um sistema baseado em agentes que, quando associados, manifestam seus próprios meios e fins. A crise das hipotecas subprime nos Estados Unidos é um bom exemplo das implicações desse tipo de relacionamento. Ela está diretamente relacionada a poderosos laços de realimentação, compostos por uma série de variáveis que amplificaram o fato de as famílias americanas terem se endividado em ritmo maior do que a sua distribuição de riqueza, prejudicada pela externalização da produção. Há atualmente uma nova estrutura na economia internacional, em que as ex-nações subdesenvolvidas estão mais bem posicionadas no cenário macroeconômico e têm maior poder do que antes - uma consequência do necessário rearranjo sistêmico. Diante dessa complexa dinâmica, a função de inteligência que deveria controlar e garantir a estabilidade sistêmica falhou em perceber a dinâmica e os impactos da nova cultura atrelada ao comportamento do capital contemporâneo, para evitar a perda da hegemonia. Observando-se ex post facto, por meio de um enfoque sistêmico, a crise econômica americana pode ser definida como tendo suas raízes nas premissas de todo o arcabouço cultural da globalização que acabou por conduzir à externalização da produção. Assim, o contexto sistêmico desta análise aponta que a cultura, como importante gerador e amplificador, deve ser o principal foco dos intentos de análise do atual contexto socioeconômico global.

  5. Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes

    Ricardo José Gunski

    1998-06-01

    Full Text Available Sequential banding analysis (Giemsa-C-banding-Ag NOR of chromosomes of the common rhea (Rhea americana was performed. Metaphases were obtained by peripheral blood lymphocyte culture and monolayer embryo cell culture. The diploid chromosome number was 80, different from the 2n = 82 in previous reports. Macrochromosome pairs 1, 2 and 5 were submetacentric and pair 3, subacrocentric. The 4th pair was acrocentric and all of the microchromosomes appeared to be acrocentric, with the exception of a clearly metacentric pair which was fully heterochromatic. The Z was slightly larger than the W, both being acrocentric and C-band negative. Nucleolar organizer regions were observed in the secondary constriction of a microchromosome pair. Correct identification of the NOR-bearing pair was possible only by sequential analyses, Giemsa staining followed by the Ag-NOR technique.Foram efetuadas análises seqüenciais de bandeamento cromossômico (Giemsa-banda-C-AgNOR em material da espécie Rhea americana (ema com o objetivo de identificar os cromossomos portadores de regiões organizadoras de nucléolos e confirmar o cariótipo desta espécie. As metáfases foram obtidas de culturas de leucócitos e de células de embrião. O número diplóide de cromossomos, determinado pela análise de metáfases oriundas de 19 espécimes, foi de 80 (2n = 80, NF = 95, o que difere da literatura. Os pares de macrocromossomos números 1, 2 e 5 eram submetacêntricos e o par 3 era sub-acrocêntrico, confirmado pelo bandeamento C. O par 4 era acrocêntrico, bem como todos os microcromossomos, com exceção de um metacêntrico inteiramente heterocromático. O cromossomo Z era ligeiramente maior que o W, sendo ambos acrocêntricos e banda-C negativos. A região organizadora de nucléolos foi observada na constrição secundária de um par de microcromossomos. A correta identificação do par portador da NOR só foi possível com a utilização da análise seqüencial de colora

  6. Tuberculosis en Rhea Americana y Gallus Sp. en cautiverio Tuberculosis in ratites and ornamental birds

    M.C Jorge

    2007-12-01

    Full Text Available La tuberculosis aviar tiene, distribución mundial y afecta a las aves domésticas y silvestres. El agente etiológico es Mycobacterium avium subsp. avium. El potencial zoonótico de esta enfermedad ha adquirido relevancia con la pandemia de HIV por ello todas las maniobras que involucren la manipulación de microorganismos viables, deben ser llevadas a cabo con adecuadas medidas de bioseguridad. La fuente primaria de infección es el ambiente contaminado con este agente. La presentación clínica es variable y el método definitivo para confirmar la infección es el aislamiento. El objetivo fue identificar mediante histopatología, bacteriología y biología molecular la etiología de lesiones compatibles con tuberculosis en una raza ornamental de Gallus sp. (sedosa del Japón y Rhea americana (ñandú. En la necropsia se observaron granulomas que presentaron centro caseonecrótico no mineralizado con bacilos ácido-alcohol resistentes en la histopatología. Se confirmó la presencia de M. avium subsp. avium por bacteriología y por PCR en las cepas aisladas. Esto permitió arribar al diagnóstico etiológico combinando técnicas y describir por primera vez en la Argentina un caso de tuberculosis en ñandú.Avian tuberculosis is worldwide distributed and affects domestic and wild birds. Aetiological agent is Mycobacterium avium subsp. avium. Its zoonotic potential has acquired importance since HIV pandemia, that is why the manipulation of viable microorganism should be done with careful biosecurity measures. Primary source of infection is the environment contaminated with these microbes. Clinical signs are not uniform and the confirmation of the disease is by bacteriologycal isolation. The purpose was to identify by histopathologycal, bacteriologycal and molecular biology methods the aetiology from tuberculosis like lesions found in an ornamental race of Gallus sp. (Japanese Silkie and Rhea americana. At post-mortem examination granulomas were

  7. Cultura e literatura latino-americanas na França (1922-1923

    Dilma Castelo Branco Diniz

    2011-11-01

    Full Text Available Resumo: Este trabalho tem por objetivo mostrar a recepção e divulgação da cultura e da literatura latino-americanas na França, realizadas através da Revue de L’Amérique Latine, nos anos de 1922 e 1923, com artigos sobre personalidades latino-americanas da política, das artes e das letras. Notícias a respeito de livros, jornais e revistas da América Latina, bem como uma crônica sobre a vida dos “Américains” em Paris fazem parte constante dos números da revista. Em razão dos limites deste estudo, dou prioridade às notícias sobre o Brasil e os brasileiros.Palavras-chave: Literatura brasileira; França; Revue de L’Amérique Latine.Résumé: Ce travail a pour but montrer la réception et la divulgation de la culture et de la littérature latino-américaines en France, réalisées à travers la Revue de l’Amérique Latine, dans les années 1922 et 1923, avec des articles concernant quelques personnalités latino-américaines de la politique, des arts et des lettres. Des nouvelles de livres, de journaux et de revues de l’Amérique Latine, et aussi une chronique sur la vie des Américains à Paris sont publiés regulièrement dans les números de la revue. En raison des limites de cette étude, j’aborde, en priorité, les nouvelles sur le Brésil et le Brésiliens.Mots-clés: Littérature brésilienne; France; Revue de L’Amérique Latine.Keywords: Brazilian literature; France; Revue de L’Amérique Latine.

  8. Effect of an admixture from Agave americana on the physical and mechanical properties of plaster

    Ochoa, J. C.

    2013-03-01

    Full Text Available Physical and mechanical properties of a plaster paste added with an organic admixture, of the leaves of Agave americana, were studied. Plastic consistency behavior was evaluated and the water/gypsum(w/g ratio was determined for each dosage of the admixture. Admixtur eeffect on setting was evaluated too. The chemical transformation of the hemihydrated form to gypsum (dihydrated form was studied using a novel technique based on a moisture analyzer by halogen light. Flexural and compressive strengths were measured. The results show that ,for the same consistency, accordingly mechanical strengths were improved too. The setting times were increased which would enhance the application time of plaster and would reduce plastic shrinkage, common problems in this type of material. The changes in these physical properties not substantially affect the final mechanical strengths.

    Se estudiaron algunas propiedades físicas y mecánicas de pastas de yeso de construcción adicionadas con un aditivo de origen orgánico, producto de las hojas de la planta Agave americana. Se evaluó el comportamiento plástico de la pasta mediante ensayos de consistencia y se determinó, para cada dosificación, su relación agua/ yeso (a/y. Se evaluó la incidencia del aditivo en los tiempos de fraguado. La transformación química del hemihidrato a yeso dihidrato se estudió mediante una novedosa técnica basada en un analizador de humedad por luz halógena. Se midieron las resistencias mecánicas a flexo-tracción y a compresión. Los resultados del estudio muestran que, para una misma consistencia, se mejoran las resistencias mecánicas. Los tiempos de fraguado de la pasta se aumentaron lo que ayudaría mejorar los tiempos de aplicación del yeso y a disminuir las retracciones plásticas. Las modificaciones de estas propiedades físicas no afectan considerablemente las resistencias mecánicas finales.

  9. Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL. λ using the prophage induction test (SOS inductest

    Juliana Brandstetter Vilar

    2009-09-01

    Full Text Available Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest. To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013, LB(1/2(malt/amp, seeded into plates with the matches, and incubated for 24 h at 37 °C. After this period, the total number of colonies and the number of plaques were counted to evaluate C. americana cytotoxicity and genotoxicity, respectively. Our results showed that although the extract of "lixeira" did not modify the survival of bacteria (p > 0.05, it caused a significant increase in prophage λ induction, especially at the higher concentrations (pCuratella americana L., comumente conhecida como "lixeira" no Brasil, é utilizada em medicina popular para tratamento de úlceras e inflamações. O presente trabalho teve como objetivo avaliar o potencial citotóxico e genotóxico do extrato etanólico das cascas de C. americana utilizando o Induteste SOS. Para avaliar a citotoxicidade da planta, depois de tratadas com diferentes concentrações do extrato, culturas de E. coli WP2s(λ foram diluνdas em tampão M9 e semeadas em placas LB. Para avaliar a genotoxicidade da planta, a cepa lisogênica WP2s(λ de E. coli foi tratada com diferentes concentrações do extrato. Em seguida, esta foi adicionada à cepa indicadora (RJF013 e ambas foram semeadas em placas em meio LB(1/2(malt(amp. Todas as culturas foram incubadas por 24

  10. Fast and Powerful: Biomechanics and Bite Forces of the Mandibles in the American Cockroach Periplaneta americana

    Weihmann, Tom; Reinhardt, Lars; Weißing, Kevin; Siebert, Tobias; Wipfler, Benjamin

    2015-01-01

    Knowing the functionality and capabilities of masticatory apparatuses is essential for the ecological classification of jawed organisms. Nevertheless insects, especially with their outstanding high species number providing an overwhelming morphological diversity, are notoriously underexplored with respect to maximum bite forces and their dependency on the mandible opening angles. Aiming for a general understanding of insect biting, we examined the generalist feeding cockroach Periplaneta americana, characterized by its primitive chewing mouth parts. We measured active isometric bite forces and passive forces caused by joint resistance over the entire mandibular range with a custom-built 2D force transducer. The opening angle of the mandibles was quantified by using a video system. With respect to the effective mechanical advantage of the mandibles and the cross-section areas, we calculated the forces exerted by the mandible closer muscles and the corresponding muscle stress values. Comparisons with the scarce data available revealed close similarities of the cockroaches’ mandible closer stress values (58 N/cm2) to that of smaller specialist carnivorous ground beetles, but strikingly higher values than in larger stag beetles. In contrast to available datasets our results imply the activity of faster and slower muscle fibres, with the latter becoming active only when the animals chew on tough material which requires repetitive, hard biting. Under such circumstances the coactivity of fast and slow fibres provides a force boost which is not available during short-term activities, since long latencies prevent a specific effective employment of the slow fibres in this case. PMID:26559671

  11. De la independencia a la natural barbarie americana. Una lectura del Facundo de Domingo F. Sarmiento

    Maura Brighenti

    2010-01-01

    Full Text Available Se presenta una lectura del "Facundo" de Domingo F. Sarmiento y, en particular, de su más general visión de la historia y de la realidad argentina y latinoamericana a partir del esquema dicotómico de civilización y barbarie que tendrá mucha suerte en las narraciones de los procesos del modernización latinoamericanos por lo menos hasta la segunda mitad del siglo XX. A través del análisis de algunos entre los múltiples niveles narrativos del "Facundo" se quiere mostrar como tal imagen dicotómica se va cristalizando en el texto. Partiendo del espacio infinito y vacío de la pampa y pasando por una antropología del gaucho - de su manera de vivir y de conducir la guerra - Sarmiento conduce el lector hasta la forma típicamente americana de despotismo, el caudillismo, llegado en Argentina a su epopeya final con el régimen de Juan M. de Rosas. En la última parte se intenta injertar el esquema dicotómico del Facundo al interior de la más general reflexión del Occidente sobre la modernidad y sus alteridades, a través por un lado del prisma orientalista y por el otro de las mismas fuentes del pensamiento moderno europeo y en particular de Locke y Hobbes.

  12. Light and scanning electron microcopy study of the tongue in Rhea americana.

    Carlesso Santos, Tatiana; Yuri Fukuda, Katia; Plácido Guimarães, Juliana; Franco Oliveira, Moacir; Angelica Miglino, Maria; Watanabe, Li-Sei

    2011-01-01

    Morphological characteristics of the tongue were studied in adult rhea (Rhea americana). The lingual surface and the surface of epithelium-connective tissue interface of rhea tongue were examined macroscopically and by light and scanning electron microscopy. The rhea tongue revealed a triangular aspect, without adjustment of the inferior bill formation, occupying approximately ¼ of the length of the oral cavity. Lingual papilla-like structures were not observed over the lingual surface. The tongue mucosa was composed of a thick non-keratinized stratified squamous epithelium in the dorsal and ventral part, supported by a connective tissue core. The submucosa contained numerous glands with cytoplasmic granules, and luminal secretion was positive for histochemical reaction to Alcian Blue in pH 2.5 and PAS, and negative to Alcian Blue in pH 0.5. Despite the rudimentary characteristic of the tongue in rhea, our results suggest an important role of tongue secretions in food lubrication and humidification during the swallowing process, based on the enormous quantity of lingual glands in the submucosa and the histochemical characteristics of their secretions.

  13. The response of dietary stressed Periplaneta americana to chronic intake of pure aflatoxin B.

    Llewellyn, G C; Sherertz, P C; Mills, R R

    1976-04-01

    In general these studies seem to indicate that adult male P. americana are not particularly sensitive, toxicologically, to aflatoxin B1, even when maintained on a marginally inadequate diet containing a low level of sucrose and no protein. Also they may be capable of detecting low levels of aflatoxin B1 in their diet (12 mug/ml) and seem not to concentrate aflatoxin B1 in their bodies. Even in dietary stressed conditions adult male American cockroaches showed a very limited potential as a bioassay organism for this toxin. Actually it appears that they may be quite resistant to the toxin. Currently there is no definite answer as to the advantages or disadvantages of insufficient dietary proteins or even carbohydrates providing protection against this toxin. The results show that the toxin would not be an effective cockroach-killing agent and thus could not serve as a bioassay system. However, this insect could serve as a model system in further investigating the mode of action and possible detoxification of aflatoxin B1.

  14. Educação em saúde: ensinando sobre a leishmaniose tegumentar americana

    Claudia Maria Antunes Uchôa

    2004-08-01

    Full Text Available Os conhecimentos sobre a circulação da leishmaniose tegumentar americana (LTA, assim como sua percepção pela população local, são de grande valia para o estabelecimento de campanhas de controle, pois mobiliza a comunidade em ações sanitárias. Este trabalho teve como objetivo esclarecer alunos e professores do ensino fundamental (1ª a 4ª série das Escolas Municipais de Maricá sobre a LTA, por meio de palestras, cartazes e cartilhas com atividades, visando à conscientização do grupo. Em sete Escolas Municipais, foram ministradas palestras e atividades a 1.314 alunos do ensino fundamental e 56 professores. Foram também distribuídas cartilhas aos alunos com atividades para a fixação do assunto. Após dois meses, uma escola foi eleita para avaliação (Escola E, sendo que, dos 213 alunos que assistiram a palestra, 198 responderam ao questionário, 178 (89,9% lembravam da doença, e 125 (63,1% responderam corretamente como ocorria a transmissão. Assim, crianças e professores informados podem funcionar como difusores da informação em Saúde na sua comunidade, sendo capazes de atuar de forma relevante no controle de endemias.

  15. Effects of phosphine on the neural regulation of gas exchange in Periplaneta americana.

    Woodman, James D; Haritos, Victoria S; Cooper, Paul D

    2008-04-01

    Phosphine is used for fumigating stored commodities, however an understanding of the physiological response to phosphine in insects is limited. Here we show how the central pattern generator for ventilation in the central nervous system (CNS) responds to phosphine and influences normal resting gas exchange. Using the American cockroach, Periplaneta americana, that perform discontinuous gas exchange (DGE) at rest, we simultaneously measure ventilatory nervous output from the intact CNS, VCO(2) and water loss from live specimens. Exposure to 800 ppm phosphine at 25 degrees C for 2 h (n=13) during recording did not cause any mortality or obvious sub-lethal effects. Within 60 s of introducing phosphine into the air flow, all animals showed a distinct CNS response accompanied by a burst release of CO(2). The initial ventilatory response to phosphine displaced DGE and was typically followed by low, stable and continuous CO(2) output. CNS output was highest and most orderly under normoxic conditions during DGE. Phosphine caused a series of ventilatory CNS spikes preceding almost complete cessation of CNS output. Minimal CNS output was maintained during the 2 h normoxic recovery period and DGE was not reinstated. VCO(2) was slightly reduced and water loss significantly lower during the recovery period compared with those rates prior to phosphine exposure. A phosphine narcosis effect is rejected based on animals remaining alert at all times during exposure.

  16. Fast and Powerful: Biomechanics and Bite Forces of the Mandibles in the American Cockroach Periplaneta americana.

    Tom Weihmann

    Full Text Available Knowing the functionality and capabilities of masticatory apparatuses is essential for the ecological classification of jawed organisms. Nevertheless insects, especially with their outstanding high species number providing an overwhelming morphological diversity, are notoriously underexplored with respect to maximum bite forces and their dependency on the mandible opening angles. Aiming for a general understanding of insect biting, we examined the generalist feeding cockroach Periplaneta americana, characterized by its primitive chewing mouth parts. We measured active isometric bite forces and passive forces caused by joint resistance over the entire mandibular range with a custom-built 2D force transducer. The opening angle of the mandibles was quantified by using a video system. With respect to the effective mechanical advantage of the mandibles and the cross-section areas, we calculated the forces exerted by the mandible closer muscles and the corresponding muscle stress values. Comparisons with the scarce data available revealed close similarities of the cockroaches' mandible closer stress values (58 N/cm2 to that of smaller specialist carnivorous ground beetles, but strikingly higher values than in larger stag beetles. In contrast to available datasets our results imply the activity of faster and slower muscle fibres, with the latter becoming active only when the animals chew on tough material which requires repetitive, hard biting. Under such circumstances the coactivity of fast and slow fibres provides a force boost which is not available during short-term activities, since long latencies prevent a specific effective employment of the slow fibres in this case.

  17. Isolation and Purification of an Antibacterial Protein from Immune Induced Haemolymph of American Cockroach, Periplaneta americana

    Hamid Reza Basseri

    2016-10-01

    Full Text Available Background: Antimicrobial peptides play a role as effectors substances in the immunity of vertebrate and inverte­brate hosts. In the current study, antimicrobial peptide was isolated from the haemolymph of the American cock­roach, Periplaneta americana.Methods: Micrococcus luteus as Gram-positive bacteria and Escherichia coli as Gram-negative bacteria were candi­date for injection. Induction was done by injecting both bacteria into the abdominal cavity of two groups of cock­roaches separately. The haemolymphs were collected 24 hours after post injection and initially tested against both bacteria. Subsequently, the immune induced haemolymph was purified by high performance liquid chromatography (HPLC to separate the proteins responsible for the antibacterial activity.Results: The non-induced haemolymph did not show any activity against both bacteria whereas induced haemo­lymph exhibited high activity against M. luteus but did less against E. coli. Two fractions showed antibacterial activ­ity against M. luteus. Finally the molecular weight of the isolated antibacterial proteins were determined as 72 kDa and 62 kDa using SDS-PAGE.Conclusion: Induced haemolymph of American cockroaches has the ability to produce peptides to combat against Gram-positive bacteria when an immune challenge is mounted. Further work has to be done to sequence of the pro­tein, which it would be advantageous.

  18. “Ser devorado no duele”. Th. W. Adorno y la experiencia americana

    Maiso, Jordi

    2009-10-01

    Full Text Available The present text aims to fathom Theodor W. Adorno’s american experience as a deciding learning process in the development of his Critical Theory. As a consequence of his New York exile years and his participance on the Princeton Radio Research Project, Adorno not only gets in touch with the proceeding of the culture industry and sets down a resolute theoretical approach to radio music, but also realizes a shift has taken place on the social function of theory which enables him a poignant self-reflection on the situation of the emigrated intellectual.El presente texto pretende desentrañar la experiencia americana de Theodor W. Adorno como un proceso de aprendizaje decisivo en la formulación de su Teoría Crítica. A partir de su exilio en Nueva York y de su participación en el Princeton Radio Research Project, Adorno no sólo adquiere un conocimiento directo del funcionamiento de la industria de la cultura y formula una decisiva aproximación teórica a la música radiofónica, sino que también toma conciencia de un cambio en la función social de la teoría que le permite llevar a cabo una aguda autorreflexión sobre la situación del intelectual emigrado.

  19. As meditações americanas de Keyserling: um cosmopolitismo nas incertezas do tempo

    Daniel Faria

    2013-12-01

    Full Text Available Hoje esquecida, a obra de Keyserling teve grande sucesso nas décadas de 1920 e 1930, com êxito editorial e repercussão entre intelectuais ao redor do mundo. No Brasil, visitado pelo conde em 1929, Keyserling foi citado em textos de Oswald e Mário de Andrade, comentários de Alceu Amoroso Lima e Lindolfo Collor, entre outros. Mário de Andrade chegou a dizer que a obra de Keyserling era a chave para a interpretação de Macunaíma.. O tema de sua obra era a decadência ocidental frente à pluralidade dos tempos históricos nas civilizações do mundo. O oriente lento, o progresso europeu e o primitivismo americano formariam uma harmonia mundial das temporalidades. Neste artigo nos deteremos nas reflexões de Keyserling e seus interlocutores, tendo em vista a questão dos mal-entendidos subjacentes a um projeto de paz mundial, baseado numa interpretação exotizante acerca das diferenças culturais, especialmente as relacionadas a uma suposta identidade sul-americana.

  20. Simultaneous analysis of glycolipids and phospholids molecular species in avocado (Persea americana Mill) fruit.

    Pacetti, Deborah; Boselli, Emanuele; Lucci, Paolo; Frega, Natale G

    2007-05-25

    The molecular species of phospholipids (PLs) and glycolipids (GLs) were simultaneously characterized in the pulp and almond of the avocado fruit (Persea americana Mill) of four varieties by means of high performance liquid chromatography-electrospray ionisation ion-trap tandem mass spectrometry. In the pulp, the predominant species of monoglycosyldiglycerides (MGD) were m/z 796.6 (oleic/linolenic and linoleic/linoleic acids) and m/z 800.4 (stearic/linoleic and oleic/oleic acids). One of the main diglycosyldiglycerides (DGD) both in the pulp and almond was m/z 958.5 (oleic/linolenic); however, the pulp was also rich of m/z 962.4 (oleic/oleic), whereas in the almond, m/z 934.5 (palmitic/linoleic and palmitoleic/oleic) and m/z 960.5 (oleic/linoleic and stearic/linolenic) were more abundant. In the almond, the main PL classes (phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI)) contained always palmitic/linoleic acids. Alpha-linolenic acid was contained as MGD (linolenic/linolenic) and DGD (linolenic/linolenic), more present in the pulp than in the almond. The major molecular species of glycocerebrosides (GCer) in the pulp and almond carried hydroxy-palmitic acid (C(16h:0))/4,8-sphyngadienine (d(18:2)).

  1. Anti Lithiasis Activity of Avocado (Persea americana Mill Leaves Extract in White Male Rats

    IETJE WIENTARSIH

    2012-03-01

    Full Text Available In Indonesia, avocado leaves have been used as traditional medicines for diureticum to cure urolithiasis. This research was to determine anti lithiasis activity of avocado leaves (Persea americana Mill extract on white male rats nefrolithiasis model induced by ethylene glycol. Ethanol extraction method was used to get extract of avogadro leaves. Twenty adult male white rats were divided into 4 different induction treatments i.e. aquadest, ethylene glycol 0.75% and ammonium chloride 2%, and extract of avocado leaves with different levels of 100 and 300 mg/kg bw respectively. Their body weight was measured daily to determine their growth ratio. And at the end of the trial, the kidney was analyzed its calcium level and inhibitory activity to formation of calcium oxalate crystals. The results showed that the amount of calcium level in the kidney of rats treated with extract of avogadro leaves was significantly decreased than that of rats treated with ethylene glycol 0.75% and ammonium chloride 2% (P < 0.05. The extract avocado leaves as a herbal remedy can be recommended as a phytotherapeutic agent especially for preventive action for urolithiasis diseases.

  2. Persea americana Mill. Seed: Fractionation, Characterization, and Effects on Human Keratinocytes and Fibroblasts.

    Ramos-Jerz, Maria Del R; Villanueva, Socorro; Jerz, Gerold; Winterhalter, Peter; Deters, Alexandra M

    2013-01-01

    Methanolic avocado (Persea americana Mill., Lauraceae) seed extracts were separated by preparative HSCCC. Partition and HSCCC fractions were principally characterized by LC-ESI-MS/MS analysis. Their in vitro influence was investigated on proliferation, differentiation, cell viability, and gene expression on HaCaT and normal human epidermal keratinocytes (NHEK) and normal human dermal fibroblasts (NHDF). The methanol-water partition (M) from avocado seeds and HSCCC fraction 3 (M.3) were mostly composed of chlorogenic acid and its isomers. Both reduced NHDF but enhanced HaCaT keratinocytes proliferation. HSCCC fraction M.2 composed of quinic acid among chlorogenic acid and its isomers inhibited proliferation and directly induced differentiation of keratinocytes as observed on gene and protein level. Furthermore, M.2 increased NHDF proliferation via upregulation of growth factor receptors. Salidrosides and ABA derivatives present in HSCCC fraction M.6 increased NHDF and keratinocyte proliferation that resulted in differentiation. The residual solvent fraction M.7 contained among low concentrations of ABA derivatives high amounts of proanthocyanidins B1 and B2 as well as an A-type trimer and stimulated proliferation of normal cells and inhibited the proliferation of immortalized HaCaT keratinocytes.

  3. Nucleotide diversity and linkage disequilibrium in wild avocado (Persea americana Mill.).

    Chen, Haofeng; Morrell, Peter L; de la Cruz, Marlene; Clegg, Michael T

    2008-01-01

    Resequencing studies provide the ultimate resolution of genetic diversity because they identify all mutations in a gene that are present within the sampled individuals. We report a resequencing study of Persea americana, a subtropical tree species native to Meso- and Central America and the progenitor of cultivated avocado. The sample includes 21 wild accessions from Mexico, Costa Rica, Ecuador, and the Dominican Republic. Estimated levels of nucleotide polymorphism and linkage disequilibrium (LD) are obtained from fully resolved haplotype data from 4 nuclear loci that span 5960 nucleotide sites. Results show that, although avocado is a subtropical tree crop and a predominantly outcrossing plant, the overall level of genetic variation is not exceptionally high (nucleotide diversity at silent sites, pi(sil) = 0.0102) compared with available estimates from temperate plant species. Intralocus LD decays rapidly to half the initial value within about 1 kb. Estimates of recombination rate (based on the sequence data) show that the rate is not exceptionally high when compared with annual plants such as wild barley or maize. Interlocus LD is significant owing to substantial population structure induced by mixing of the 3 botanical races of avocado.

  4. Persea americana (avocado): bringing ancient flowers to fruit in the genomics era.

    Chanderbali, André S; Albert, Victor A; Ashworth, Vanessa E T M; Clegg, Michael T; Litz, Richard E; Soltis, Douglas E; Soltis, Pamela S

    2008-04-01

    The avocado (Persea americana) is a major crop commodity worldwide. Moreover, avocado, a paleopolyploid, is an evolutionary "outpost" among flowering plants, representing a basal lineage (the magnoliid clade) near the origin of the flowering plants themselves. Following centuries of selective breeding, avocado germplasm has been characterized at the level of microsatellite and RFLP markers. Nonetheless, little is known beyond these general diversity estimates, and much work remains to be done to develop avocado as a major subtropical-zone crop. Among the goals of avocado improvement are to develop varieties with fruit that will "store" better on the tree, show uniform ripening and have better post-harvest storage. Avocado transcriptome sequencing, genome mapping and partial genomic sequencing will represent a major step toward the goal of sequencing the entire avocado genome, which is expected to aid in improving avocado varieties and production, as well as understanding the evolution of flowers from non-flowering seed plants (gymnosperms). Additionally, continued evolutionary and other comparative studies of flower and fruit development in different avocado strains can be accomplished at the gene expression level, including in comparison with avocado relatives, and these should provide important insights into the genetic regulation of fruit development in basal angiosperms.

  5. Transcriptional signatures of ancient floral developmental genetics in avocado (Persea americana; Lauraceae).

    Chanderbali, André S; Albert, Victor A; Leebens-Mack, Jim; Altman, Naomi S; Soltis, Douglas E; Soltis, Pamela S

    2009-06-02

    The debate on the origin and evolution of flowers has recently entered the field of developmental genetics, with focus on the design of the ancestral floral regulatory program. Flowers can differ dramatically among angiosperm lineages, but in general, male and female reproductive organs surrounded by a sterile perianth of sepals and petals constitute the basic floral structure. However, the basal angiosperm lineages exhibit spectacular diversity in the number, arrangement, and structure of floral organs, whereas the evolutionarily derived monocot and eudicot lineages share a far more uniform floral ground plan. Here we show that broadly overlapping transcriptional programs characterize the floral transcriptome of the basal angiosperm Persea americana (avocado), whereas floral gene expression domains are considerably more organ specific in the model eudicot Arabidopsis thaliana. Our findings therefore support the "fading borders" model for organ identity determination in basal angiosperm flowers and extend it from the action of regulatory genes to downstream transcriptional programs. Furthermore, the declining expression of components of the staminal transcriptome in central and peripheral regions of Persea flowers concurs with elements of a previous hypothesis for developmental regulation in a gymnosperm "floral progenitor." Accordingly, in contrast to the canalized organ-specific regulatory apparatus of Arabidopsis, floral development may have been originally regulated by overlapping transcriptional cascades with fading gradients of influence from focal to bordering organs.

  6. Microsatellite markers in avocado (Persea americana Mill.): genealogical relationships among cultivated avocado genotypes.

    Ashworth, V E T M; Clegg, M T

    2003-01-01

    Twenty-five microsatellite markers uniquely differentiated 35 avocado cultivars and two wild relatives. Average heterozygosity was high (60.7%), ranging from 32% in P. steyermarkii to 84% in Fuerte and Bacon. In a subset of 15 cultivars, heterozygosity averaged 63.5% for microsatellites, compared to 41.8% for restriction fragment length polymorphisms (RFLPs). A neighbor-joining tree, according to average shared allele distances, consisted of three clusters likely corresponding to the botanical races of avocado and intermediate clusters uniting genotypes of presumably racially hybrid origin. Several results were at odds with existing botanical assignments that are sometimes rendered difficult by incomplete pedigree information, the complexity of the hybrid status (multiple backcrossing), or both. For example, cv. Harvest clustered with the Guatemalan race cultivars, yet it is derived from the Guatemalan x Mexican hybrid cv. Gwen. Persea schiedeana grouped with cv. Bacon. The rootstock G875 emerged as the most divergent genotype in our data set. Considerable diversity was found particularly among accessions from Guatemala, including G810 (West Indian race), G6 (Mexican race), G755A (hybrid Guatemalan x P. schiedeana), and G875 (probably not P. americana). Low bootstrap support, even upon exclusion of (known) hybrid genotypes from the data matrix, suggests the existence of ancient hybridization or that the botanical races originated more recently than previously thought.

  7. Bitter-tasting and kokumi-enhancing molecules in thermally processed avocado (Persea americana Mill.).

    Degenhardt, Andreas Georg; Hofmann, Thomas

    2010-12-22

    Sequential application of solvent extraction and RP-HPLC in combination with taste dilution analyses (TDA) and comparative TDA, followed by LC-MS and 1D/2D NMR experiments, led to the discovery of 10 C(17)-C(21) oxylipins with 1,2,4-trihydroxy-, 1-acetoxy-2,4-dihydroxy-, and 1-acetoxy-2-hydroxy-4-oxo motifs, respectively, besides 1-O-stearoyl-glycerol and 1-O-linoleoyl-glycerol as bitter-tasting compounds in thermally processed avocado (Persea americana Mill.). On the basis of quantitative data, dose-over-threshold (DoT) factors, and taste re-engineering experiments, these phytochemicals, among which 1-acetoxy-2-hydroxy-4-oxo-octadeca-12-ene was found with the highest taste impact, were confirmed to be the key contributors to the bitter off-taste developed upon thermal processing of avocado. For the first time, those C(17)-C(21) oxylipins exhibiting a 1-acetoxy-2,4-dihydroxy- and a 1-acetoxy-2-hydroxy-4-oxo motif, respectively, were discovered to induce a mouthfulness (kokumi)-enhancing activity in sub-bitter threshold concentrations.

  8. Effect of a novel oil extraction method on avocado (Persea americana Mill) pulp microstructure.

    Ortiz, M Alicia; Dorantes, A Lidia; Gallndez, M Juvencio; Cardenas, S Elizabeth

    2004-01-01

    Avocado (Persea americana Mill) is an oil-rich fruit, the pulp containing up to 33% of the oil. It is rich in monounsaturated fatty acids, and has nutritional properties similar to olive oil. However, there is no widespread commercial method for oil recovery from avocado pulp. The aim of this study is to contribute to the limited knowledge about the micro- and ultrastructure of avocado. It presents a micro- and ultrastructural study of avocado pulp before and after three different oil recovery methods, in order to relate the quality and yielding of the oil to the cellular changes in the pulp. This study was made using light, scanning electron, and electron transmission microscopy. The microwave-squeezing method yielded 67% of the oil, preserved the shape of the cell by causing only a slight modification, and gave the best quality oil. Hexane extraction yielded 59%, causing the idioblastic oil cells to become irregularly shaped and rough-surfaced. Acetone extraction yielded 12%, and deformed the cellular wall while the oil remained inside, giving a poor quality oil. On the basis of these results, the microwave-squeezing method is suggested as a new option for oil recovery from avocadopulp. This method could be adapted for industrial processing.

  9. Estudios sobre Trypanosomiasis americana en el Perú. Observaciones en el departamento de Ica

    Víctor M Ayulo Robles

    1946-01-01

    Full Text Available Hemos comprobado la presencia de Triatomas en las ciudades de Ica y Nazca. Los Triatomas encontrados en esas localidades son de la especie del Triatoma Infestans. Las observaciones del contenido intestinal de los Triatomas capturados en ambas ciudades reveló que ellos no se encuentran infestados; lo que hace sospechar que estos insectos procedan de la Ciudad de Arequipa. Los exámenes de sangre en fresco, gota gruesa y frotis coloreados por el método de Giemsa en 174 personas y 32 animales, así como las pruebas de Desviación del Complemento (Reacción de GUERREIRO Y MACHADO e intradermo-reacción en las 174 personas, fueron negativas; lo que demuestra que actualmente no existe la Enfermedad de Chagas en el Departamento de Ica. Dada la presencia de Triatomas en Ica y Nazca y la existencia de la Enfermedad de Chagas en Vitor, Sihuas y Quishuarani del Departamento de Arequipa no se excluye la posibilidad de que posteriormente se haga presente la Trypanosomiasis americana, también, en esas zonas.

  10. MISSIONÁRIAS PROTESTANTES AMERICANAS (1870 – 1920: GÊNERO, CULTURA, HISTÓRIA

    Eliane Moura da Silva

    2011-01-01

    Full Text Available Esse artigo trata as relações entre religião, cultura e papéis de gênero entre 1870 e 1920 tendo como objeto de estudo as missionárias protestantes americanas durante colonialismo e imperialismo. Trabalhando com a perspectiva das relações entre gênero, cultura e religião têm como objetivo apresentar algumas reflexões teóricas sobre a história cultural das religiões e de gênero. Parte-se do pressuposto analítico de que as missões cristãs em geral, e as protestantes em particular, foram mais do que colonizações culturais: a mesma missão que converteu o Outro trouxe o Outro para dentro de si e construíram novas culturas e fronteiras e relações entre gênero e religião.

  11. Plantio direto de alface americana sobre plantas de cobertura dessecadas ou roçadas

    Andréia Cristina Silva Hirata

    2014-06-01

    Full Text Available O objetivo deste trabalho foi avaliar o plantio direto de alface americana no verão sobre plantas de cobertura dessecadas ou roçadas em cultivos sucessivos. O trabalho foi desenvolvido no município de Álvares Machado, São Paulo, Brasil. O delineamento experimental foi em blocos ao acaso, dispostos em parcelas subdivididas, com quatro repetições. O fator da parcela principal foram dois manejos das plantas de cobertura (dessecadas com herbicida ou roçadas e as subparcelas, seis espécies de plantas de cobertura (Cajanus cajan cv. IAPAR 43, Crotalaria spectabilis, Crotalaria juncea, Mucuna pruriens, Pennisetum glaucum e vegetação natural, além da testemunha sem plantas de cobertura. O manejo roçado apresentou desempenho inferior no primeiro cultivo da alface, todavia não diferiu do manejo químico no segundo cultivo. A cobertura do solo com mucuna apresentou maior produtividade da alface no primeiro cultivo devido ao excesso de palha das demais coberturas, o qual prejudicou o estabelecimento da alface. No segundo cultivo não houve diferenças entre as coberturas. A roçagem de plantas de cobertura é uma opção viável para plantio direto de alface sem herbicidas.

  12. GIBBERELLINS, FUNGICIDES AND STORAGE EFFECTS ON THE GERMINATION OF Genipa americana L. (RUBIACEAE SEEDS

    Fábio de Almeida Vieira

    2006-06-01

    Full Text Available The aim of this paper was to verify the effect of different doses of gibberellic acid (GA3 (0, 250, 500, 750 and 1000 µg.L-1, of fungicides of the groups chemical benzimidazol (0, 25, 50 and 100 g.L-1 and ditiocarbamato (0, 1,25, 2,50 and 5,00 g.L-1 on seed germination. Viability of those seeds was evaluated through germination tests at 0, 15, 30 and 60 days. The experiment was carried out in greenhouse. The experimental design was fully randomized one, with five replicates per treatment. The traits evaluated were emergence and index of emergence speed. The treatment with GA3 didn't provide significant so much differences among the germination rates as well as for the emergence speed. It was verified that the use of the fungicides in smaller concentrations (25 g.L-1 of benzimidazol and 1,25 g.L-1 of ditiocarbamato promoted a better germination speed. The seeds of G. americana possess viability period relatively short, with germination absence 60 days period of storage, and it could be associated to the humidity tenors presented by the seeds in this period.

  13. Expression of a Ripening-Related Avocado (Persea americana) Cytochrome P450 in Yeast.

    Bozak, K R; O'keefe, D P; Christoffersen, R E

    1992-12-01

    One of the mRNAs that accumulates during the ripening of avocado (Persea americana Mill. cv Hass) has been previously identified as a cytochrome P450 (P450) monooxygenase and the corresponding gene designated CYP71A1. In this report we demonstrate that during ripening the accumulation of antigenically detected CYP71A1 gene product (CYP71A1) correlates with increases in total P450 and two P450-dependent enzyme activities: para-chloro-N-methylaniline demethylase, and trans-cinnamic acid hydroxylase (tCAH). To determine whether both of these activities are derived from CYP71A1, we have expressed this protein in yeast (Saccharomyces cerevisiae) using a galactose-inducible yeast promoter. Following induction, the microsomal fraction of transformed yeast cells undergoes a large increase in P450 level, attributable almost exclusively to the plant CYP71A1 protein. These membranes exhibit NADPH-dependent para-chloro-N-methylaniline demethylase activity at a rate comparable to that in avocado microsomes but have no detectable tCAH. These results demonstrate both that the CYP71A1 protein is not a tCAH and that a plant P450 is fully functional upon heterologous expression in yeast. These findings also indicate that the heterologous P450 protein can interact with the yeast NADPH:P450 reductase to produce a functional complex.

  14. Persea americana Mill. Seed: Fractionation, Characterization, and Effects on Human Keratinocytes and Fibroblasts

    Maria del R. Ramos-Jerz

    2013-01-01

    Full Text Available Methanolic avocado (Persea americana Mill., Lauraceae seed extracts were separated by preparative HSCCC. Partition and HSCCC fractions were principally characterized by LC-ESI-MS/MS analysis. Their in vitro influence was investigated on proliferation, differentiation, cell viability, and gene expression on HaCaT and normal human epidermal keratinocytes (NHEK and normal human dermal fibroblasts (NHDF. The methanol-water partition (M from avocado seeds and HSCCC fraction 3 (M.3 were mostly composed of chlorogenic acid and its isomers. Both reduced NHDF but enhanced HaCaT keratinocytes proliferation. HSCCC fraction M.2 composed of quinic acid among chlorogenic acid and its isomers inhibited proliferation and directly induced differentiation of keratinocytes as observed on gene and protein level. Furthermore, M.2 increased NHDF proliferation via upregulation of growth factor receptors. Salidrosides and ABA derivatives present in HSCCC fraction M.6 increased NHDF and keratinocyte proliferation that resulted in differentiation. The residual solvent fraction M.7 contained among low concentrations of ABA derivatives high amounts of proanthocyanidins B1 and B2 as well as an A-type trimer and stimulated proliferation of normal cells and inhibited the proliferation of immortalized HaCaT keratinocytes.

  15. Optimization of Insoluble and Soluble Fibres Extraction from Agave americana L. Using Response Surface Methodology

    Amine Bouaziz

    2014-01-01

    Full Text Available Experimental design methodology was used to determine significant factors affecting the extraction yield of soluble and insoluble fibres from Agave americana L. and in second time to find optimum conditions leading to the highest yield. Results clearly indicated that the temperature, the powder to water (P/W ratio, and the agitation speed were the most important factors influencing fibres extraction yield which increased with temperature, P/W ratio, and agitation speed. Ionic strength affected significantly soluble fibre extraction yield and was the most important factor among nonsignificant ones influencing insoluble fibres extraction yield. Then, a Box-Behnken design was carried out to maximise fibres extraction. Selected optimal conditions were temperature: 90°C; P/W ratio: 0.1625; agitation speed: 400 rpm; and ionic strength: 1.5 g/L. These conditions yielded 93.02% and 80.46% of insoluble and soluble fibres, respectively. Concentrates showed high fibres purity and good functional properties.

  16. Four new steroid constituents from the waste residue of fibre separation from Agave americana leaves.

    Jin, Jian-Ming; Zhang, Ying-Jun; Yang, Chong-Ren

    2004-06-01

    Three new steroidal saponins, named agamenosides H-J (1-3), and a new cholestane steroid agavegenin D (4) were isolated from the waste residue of fibre separation from Agave americana leaves, together with six known steroids. Structures of the new compounds 1-4 were deduced to be (22S,23S,24R,25S)-24-[(beta-D-glucopyranosyl)oxy]-5alpha-spirostane-3beta,6alpha,23-triol 6-O-beta-D-glucopyranoside (1), (22S,23S,24R,25S)-5alpha-spirostane-3beta,23,24-triol 24-O-beta-D-glucopyranoside (2), (22S,23S,25R,26S)-23,26-epoxy-5alpha-furostane-3beta,22,26-triol 26-O-beta-D-glucopyranoside (3), and (22S,25S)-5alpha-cholestane-3beta,16beta,22,26-tetrol (4), respectively, by means of spectroscopic analysis, including extensive 1D and 2D NMR data, and the results of hydrolytic cleavage.

  17. Genomics-assisted characterization of a breeding collection of Apios americana, an edible tuberous legume

    Belamkar, Vikas; Farmer, Andrew D.; Weeks, Nathan T.; Kalberer, Scott R.; Blackmon, William J.; Cannon, Steven B.

    2016-01-01

    For species with potential as new crops, rapid improvement may be facilitated by new genomic methods. Apios (Apios americana Medik.), once a staple food source of Native American Indians, produces protein-rich tubers, tolerates a wide range of soils, and symbiotically fixes nitrogen. We report the first high-quality de novo transcriptome assembly, an expression atlas, and a set of 58,154 SNP and 39,609 gene expression markers (GEMs) for characterization of a breeding collection. Both SNPs and GEMs identify six genotypic clusters in the collection. Transcripts mapped to the Phaseolus vulgaris genome–another phaseoloid legume with the same chromosome number–provide provisional genetic locations for 46,852 SNPs. Linkage disequilibrium decays within 10 kb (based on the provisional genetic locations), consistent with outcrossing reproduction. SNPs and GEMs identify more than 21 marker-trait associations for at least 11 traits. This study demonstrates a holistic approach for mining plant collections to accelerate crop improvement. PMID:27721469

  18. Mechanical properties of tracheal tubes in the American cockroach (Periplaneta americana)

    Webster, Matthew R.; De Vita, Raffaella; Twigg, Jeffrey N.; Socha, John J.

    2011-09-01

    Insects breathe using an extensive network of flexible air-filled tubes. In some species, the rapid collapse and reinflation of these tubes is used to drive convective airflow, a system that may have bio-inspired engineering applications. The mechanical behavior of these tracheal tubes is critical to understanding how they function in this deformation process. Here, we performed quasi-static tensile tests on ring sections of the main thoracic tracheal trunks from the American cockroach (Periplaneta americana) to determine the tracheal mechanical properties in the radial direction. The experimental findings indicate that the stress-strain relationships of these tracheal tubes exhibit some nonlinearities. The elastic modulus of the linear region of the stress-strain curves tubes was found to be 1660 ± 512 MPa. The ultimate tensile strength, ultimate strain and toughness were found to be 23.7 ± 7.33 MPa, 2.0 ± 0.7% and 0.207 ± 0.153 MJ m-3, respectively. This study is the first experimental quantification of insect tracheal tissue, and represents a necessary step toward understanding the mechanical role of tracheal tubes in insect respiration.

  19. A figura do gaúcho e a identidade cultural latino-americana

    Freitas, Letícia Fonseca Richthofen de

    2004-01-01

    Full Text Available Este trabalho tem por objetivo examinar, a partir da análise de reportagens de jornais de Porto Alegre – Zero Hora e Correio do Povo – publicadas durante as comemorações da Semana Farroupilha de 2003, a figura do gaúcho como um dos ícones da identidade sul-rio-grandense, considerando-a uma das possíveis identidades latino-americanas. A análise considera o papel pedagógico exercido pela mídia no sentido de instituir verdades e produzir subjetividades, ensinando determinadas maneiras de se ser gaúcho. O referencial teórico da pesquisa está situado no campo dos Estudos Culturais, cujos conceitos-chave são justamente cultura, identidade, sistemas de significação e poder. O hibridismo cultural da identidade gaúcha, num embate com as identidades nacionais (brasileira e uruguaia, assim como o destaque dado às crianças, em uma perspectiva pedagógica, são discutidos em três reportagens publicadas no mês de setembro de 2003 nos jornais citados

  20. Potencial alelopático de plantas de acapu (Vouacapoua americana: efeitos sobre plantas daninhas de pastagens Allelopathic potential of "acapu" (Vouacapoua americana plants: effects on pasture weed

    A.P.S. Souza Filho

    2000-01-01

    Full Text Available Extratos aquosos de folhas e cascas de plantas de acapu (Vouacapoua americana foram preparados nas concentrações de 0, 1, 2, 3 e 5% (v/v, visando identificar e caracterizar a atividade potencialmente alelopática dessa espécie. Analisaram-se os efeitos dos extratos sobre a germinação de sementes e o alongamento da raiz primária das plantas daninhas malícia (Mimosa pudica e malva (Urena lobata. Os bioensaios de germinação foram desenvolvidos em condições de 25 ºC e fotoperíodo de 12 horas. Para os bioensaios de alongamento da raiz primária, as condições estabelecidas foram de 25 ºC e fotoperíodo de 24 horas. Os resultados obtidos indicaram variações de respostas em função da fonte do extrato aquoso, do parâmetro analisado e da concentração do extrato. As reduções observadas tanto na germinação como no alongamento da raiz primária foram crescentes com o aumento da concentração do extrato, sendo os efeitos mais intensos observados na concentração de 5%. Independentemente da espécie receptora e do parâmetro analisado, o extrato preparado a partir das cascas do acapu evidenciou maior atividade alelopática inibitória. O alongamento da raiz primária foi o parâmetro mais sensível aos efeitos potencialmente alelopáticos do que a germinação das sementes. Comparativamente, cascas e folhas apresentaram diferenças em relação às classes de substâncias químicas. Nas cascas foram encontradas cumarinas que não estavam presentes nas folhas, as quais, por sua vez, apresentaram esteróides e triterpenóides, que não foram identificados nas cascas do acapu.Aqueous extracts from leaves and bark of "Acapu" plants were prepared in concentration levels of 0, 1, 2, 3 and 5% to identify and characterize potential allelopathic activity of such plants. The extract effects on seed germination and primary root elongation of pasture weeds, such as Mimosa pudica and Urena lobata, were analyzed. The germination bioassays were

  1. La inscripción de la identidad en la obra literaria: Análisis comparativo de “Lejana” de Julio Cortázar y La muerte salió cabalgado de Persia de Péter Hajnóczy

    Zsuzsanna Szalai

    2010-07-01

    Full Text Available Le nom de Julio Cortázar est incontestablement lié à l’idée de la rénovation du roman espagnol. L’auteur hongrois Péter Hajnóczy (1942-1981 est peut-être moins mondialement connu, mais sa technique narrative inspirée du style de Faulkner mérite l’attention. Dans la perspective de notre interprétation, un procédé technique très caractéristique des deux écrivains est envisagé : la multiplication, un instrument de réflexions et de projections. Nous analyserons parallèlement « Lejana » de Cortázar et A halál kilovagolt Perzsiából (1979 de Hajnóczy afin d’étudier la multiplication de l’identité, des espaces, du temps, des niveaux textuels, etc. Notre fil conducteur sera l’étude de la métalittérature : au cours de la lecture, nous étudierons la naissance du texte littéraire à travers l’analyse de la position des deux auteurs implicites, de leurs doutes et de leurs difficultés. Enfin, nous examinerons la relation interne entre le dénouement et la structure de chacune des œuvres. Comme il s’agit, en un certain sens, de deux histoires autobiographiques, leur dénouement promet d’être problématique.The name of Julio Cortázar undoubtedly imposes the idea of the Hispanic novel’s renovation. The Hungarian author Péter Hajnóczy (1942-1981 is perhaps less known worldwide, but his Faulkner style narrative technique deserves more attention. In the focus of the present interpretation, a very characteristic technical process of the two writers is featured: the multiplication, an instrument of reflections and projections. We analyse abreast “Far” (“Lejana” of Cortázar and Death Rode Out of Persia (1979 of Hajnóczy to discover the multiplication of identity, spaces, time and text levels, etc. Our principal line will be the study of the metaliterature: during the reading we will continue the birth of the literary text, observe doubts and difficulties of the two represented implicit authors

  2. Mito e sociedade na literatura latino-americana: Neruda, Carpentier, Bolaño

    Pedro Dolabela Chagas

    2013-09-01

    Full Text Available Resumo: Discussão sobre a problemática identitária na literatura latino-americana: a sua associação oitocentista à representação da natureza e da paisagem social; a sua longa duração entre os séculos XIX e XX; a sua politização pela dicotomia entre a América Latina e o Centro representado pela Europa e pelos EUA. A construção da identidade como produção de mitos: como construção de passados úteis, valoração positiva ou negativa do presente, projeção de futuros possíveis. A presença do mito em Canto geral, de Pablo Neruda; a sua recusa em 2666, de Roberto Bolaño; discussão sobre as razões desta recusa: a concentração no presente em detrimento da projeção do futuro. Localização de um tensionamento embrionário do mito identitário em Os passos perdidos, de Alejo Carpentier: discussão sobre os termos de orientação deste tensionamento e a diferença que ele produzia em relação à história anterior do romance latino-americano. Sugestão do fechamento recente da época histórica dominada pela problemática identitária na narrativa latino-americana.Palavras-chave: Pablo Neruda; Alejo Carpentier; Roberto Bolaño; romance latino-americano; mito e identidade. Abstract: The problematic of identity representation in Latin- American literature: its 19th Century association to the representation of nature and the social landscape; its long duration in the 19th and 20th Centuries; its politicization through the dichotomy between Latin America and a Center represented by Europe and the USA. Identity construction as a production of myths: as a construction of usable pasts, as a positive or negative evaluation of the present, as a projection of possible futures. The presence of myth in Pablo Neruda’s Canto general; its rejection in Roberto Bolaño’s 2666; the possible reasons of this rejection: a concentration in the present in detriment of the future. Location of an embrionary tensioning of the identity myths

  3. Symbiotic essential amino acids provisioning in the American cockroach, Periplaneta americana (Linnaeus under various dietary conditions

    Paul A. Ayayee

    2016-05-01

    Full Text Available Insect gut microbes have been shown to provide nutrients such as essential amino acids (EAAs to their hosts. How this symbiotic nutrient provisioning tracks with the host’s demand is not well understood. In this study, we investigated microbial essential amino acid (EAA provisioning in omnivorous American cockroaches (Periplaneta americana, fed low-quality (LQD and comparatively higher-quality dog food (DF diets using carbon stable isotope ratios of EAAs (δ13CEAA. We assessed non-dietary EAA input, quantified as isotopic offsets (Δ13C between cockroach (δ13CCockroach EAA and dietary (δ13CDietary EAA EAAs, and subsequently determined biosynthetic origins of non-dietary EAAs in cockroaches using 13C-fingerprinting with dietary and representative bacterial and fungal δ13CEAA. Investigation of biosynthetic origins of de novo non-dietary EAAs indicated bacterial origins of EAA in cockroach appendage samples, and a mixture of fungal and bacterial EAA origins in gut filtrate samples for both LQD and DF-fed groups. We attribute the bacteria-derived EAAs in cockroach appendages to provisioning by the fat body residing obligate endosymbiont, Blattabacterium and gut-residing bacteria. The mixed signatures of gut filtrate samples are attributed to the presence of unassimilated dietary, as well as gut microbial (bacterial and fungal EAAs. This study highlights the potential impacts of dietary quality on symbiotic EAA provisioning and the need for further studies investigating the interplay between host EAA demands, host dietary quality and symbiotic EAA provisioning in response to dietary sufficiency or deficiency.

  4. A cultura de belezas americanas: gestão de pessoas, discurso e sujeito

    Fabio Bittencourt Meira

    Full Text Available O discurso organizacional parece incapaz de induzir comportamentos alinhados com as exigências e necessidades da empresa. A literatura sobre gestão de pessoas aponta essa dissociação entre discurso e prática como um paradoxo. Este artigo propõe ir além, ao considerar que ele é, na verdade, signo de uma contradição constitutiva do campo a partir da qual as ações gerenciais são produzidas. A não coincidência entre discurso e prática é a regra que propicia a regeneração e reiteração incessante do que é comunicado. Técnicas renovadas de treinamento mostram que a integração dos indivíduos é vista como um problema de aprendizagem, dependente do ensino de modos de sentir e perceber suas sensações e experiências, o que vai muito além da fronteira do discurso, apontando outros limites e antagonistas. Espera-se que os indivíduos sejam sujeitos de sua própria sujeição. Nessa perspectiva, a operação e o problema da gestão de pessoas residem na necessidade de produzir simultaneamente a completa sujeição e o sujeito pleno. Os referenciais teóricos do discurso organizacional e da teoria linguística fundamentam a crítica às práticas inovadoras de gestão de pessoas. Um dos personagens do filme Beleza americana ajuda a reordenar a reflexão anterior, propiciando a retomada do problema segundo a nova chave, com a passagem do discurso ao sujeito.

  5. Aerobic Bacterial Community of American Cockroach Periplaneta americana,a Step toward Finding Suitable Paratransgenesis Candidates

    Sanaz Akbari

    2015-10-01

    Full Text Available Background: Cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. Identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. Here the bacterial microbiota of Periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate.Methods: Midgut of specimens were dissected and cultivated in different media. The bacterial isolates were then identified using the phenotypic and 16S-rRNA sequencing methods.Results: The analytical profile index (API kit showed presence of 11 bacterial species including: Escherichia coli, Shigella flexineri, Citrobacter freundii, E. vulneris, Enterobacter cloacae, Yersinia pseudotuberculosis, Y.intermedia, Leclericia adecarboxylata, Klebsiella oxytoca, K. planticola, and Rahnella aquatilis in the cockroach midguts. The first three species are potentially symbiotic whereas others are transient. The conventional platingmethod revealed presence of only four isolates of Salmonella, E. coli, and Proteus which in three cases mismatched with API and 16S-rRNA genotyping. The API correctly identified the four isolates as Shigella flexneri, Citrobacter freundii, and E. coli (n= 2. 16S-rRNA sequence analysis confirmed the API results; however the C. freundii sequencewas identical with C. murliniae indicating lack of genetic variation in the gene between these two closely related species.Conclusion: A low number of potentially symbiotic bacteria were found in the American cockroach midguts. Among them Enterobacter cloacae is a potential candidate for paratransgenesis approach whereas other bacteria are pathogens and are not useful for the approach. Data analysis showed that identification levels increase from the conventional to API and to genotyping respectively.

  6. Leishmaniasis tegumentaria americana: encuesta epidemiológica en una comunidad indígena

    Martha Wolff

    1988-01-01

    Full Text Available Se realizó un estudio epidemiológico sobre Leishmaniasis tegumentarla americana (LTA en 809 personas del Resguardo Indígena de San Andrés de Sotavento, Departamento de Córdoba, foco endémico tanto de Leishmaniasis cutánea como de Leishmaniasis visceral. La distribución por sexo de la población encuestada fue de 355 (43,9% hombres y 454 (56, 1% mujeres; sus índices alérgicos fueron de 36,9% y 40,5% respectivamente. La positividad de la Intradermorreacción de Montenegro ascendió en personas de ambos sexos, a medida que aumentó la edad. El índice parasitario fue de 0.37%. La especie de Lutzomyia más frecuente en el intra y peridomicilio fue la Lu. evansi que mostró comportamiento antropofílico. Se encontró una alta exposición de la población general al vector dada la facilidad de penetración de los flebotomineos alintradomicilio, por el tipo de construcción de las viviendas; además, al no existir servicios sanitarios, acueductos ni alcantarillados Intradomiciliarios, todo el núcleo familiar debe desplazarse al peridomicilio para la disposición de excretas y en busca del agua. Otros hallazgos fueron el gran desconocimiento sobre la etiología y tratamiento de la Leishmaniasis y las pésimas condiciones sociales y económicas de la región.

  7. Leishmaniose tegumentar americana no Distrito Federal Mucocutaneous leishmaniasis in the Federal District

    Raimunda Nonata Ribeiro Sampaio

    1999-10-01

    Full Text Available Relatamos aqui 11 casos de leishmaniose tegumentar americana (LTA em pacientes que residem no DF e que não saíram da sua área durante um tempo que variou de seis meses a dois anos antes do início da doença. Seis dos 11 pacientes, residem na cidade satélite de Planaltina. Todos têm a intradermorreação de Montenegro positiva. Dez deles têm presença de leishmânia nas lesões. Nas lesões de dois pacientes foram identificadas, pelo método de anticorpos monoclonais, Leishmania (V braziliensis. Nove deles, foram tratados com antimonial pentavalente e dois com pentamidina. Houve duas ocorrências de recidiva, ambas, após o uso do antimonial. Constatada a presença de vetores e de pacientes infectados no Distrito Federal, acredita-se que possa estar ocorrendo infecção por leishmânia em Brasília e em suas áreas periurbanas.In the present study, we report 11 cases of mucocutaneous leishmaniasis (MCL in patients living in the Federal District who had not left their area of residence for a period ranging from six months to two years before the onset of the disease. Six of the eleven patients live in the suburban town of Planaltina. All showed a positive Leishmanin intradermal reaction. Ten of them presentedt the parasite in their lesions. Leishmania (V braziliensi was identified in the lesions of two patients by the monoclonal antibody method. Nine patients were treated with pentavalent antimoy and two with pentamidine. Relapse occurred in two cases after treatment with antimony. In view of the detection of vectors and infected patients in the Federal District, it is probable that Leishmania infection is occurring in Brasilia and its suburban areas.

  8. Nonlinear cable properties of the giant axon of the cockroach Periplaneta americana.

    Segev, I; Parnas, I

    1985-05-01

    The steady state nonlinear properties of the giant axon membrane of the cockroach Periplaneta americana were studied by means of intracellular electrodes. The resistivity of this membrane markedly decreases in response to small subthreshold depolarizations. The specific slope resistance is reduced by twofold at 5 mV depolarization and by a factor of 14 at 20 mV depolarization. As a result, the spatial decay, V(X), of depolarizing potentials is enhanced when compared with the passive (exponential) decay. This enhancement is maximal at a distance of 1-1.5 mm from a point of subthreshold (0-20 mV) depolarizing perturbation. At that distance, the difference between the actual potential and the potential expected in the passive axon is approximately 30%. The effects of membrane rectification on V(X) were analyzed quantitatively with a novel derivation based on Cole's theorem, which enables one to calculate V(X) directly from the input current-voltage (I0-V) relation of a long axon. It is shown that when the experimental I0-V curve is replotted as (I0Rin)-1 against V (where Rin is the input resistance at the resting potential), the integral between any two potentials (V1 greater than V2) on this curve is the distance, in units of the resting space constant, over which V1 attenuates to V2. Excellent agreement was found between the experimental V(X) and the predicted value based solely on the input I0-V relation. The results demonstrate that the rectifying properties of the giant axon membrane must be taken into account when the electrotonic spread of even small subthreshold potentials is studied, and that, in the steady state, this behavior can be extracted from measurements at a single point. The effect of rectification on synaptic efficacy is also discussed.

  9. Terpenoid variations within and among half-sibling avocado trees, Persea americana Mill. (Lauraceae).

    Niogret, Jerome; Epsky, Nancy D; Schnell, Raymond J; Boza, Edward J; Kendra, Paul E; Heath, Robert R

    2013-01-01

    Chemical analyses were conducted to determine the qualitative and quantitative differences in monoterpenes and sesquiterpenes in plant material from avocado trees, Persea americana Mill. (Lauraceae). The initial study analyzed plant material sampled from the trunk to the leaves through different branch diameters to quantify proximo-distal spatial differences within a tree. All trees were seedlings initiated from a single maternal tree. Two-way analysis of variance was conducted on 34 chemicals that comprised at least 3% of the total chemical content of at least one tree and/or location within a tree. There were significant interactions between genotype and location sampled for most chemicals. Parentage analysis using microsatellite molecular markers (SSR's) determined that the four trees had three fathers and that they represented two full-siblings and two half-sibling trees. Descriptive discriminant analysis found that both genotype and location within a tree could be separated based on chemical content, and that the chemical content from full-siblings tended to be more similar than chemical content from half-siblings. To further explore the relationship between genetic background and chemical content, samples were analyzed from leaf material from 20 trees that included two sets of full-sibling seedling trees, the maternal tree and the surviving paternal tree. Descriptive discriminant analysis found good separation between the two full-sibling groups, and that the separation was associated with chemistry of the parental trees. Six groups of chemicals were identified that explained the variation among the trees. We discuss the results in relation to the discrimination process used by wood-boring insects for site-selection on host trees, for tree selection among potential host trees, and the potential use of terpenoid chemical content in chemotaxonomy of avocado trees.

  10. Antidiabetic principles ofLoranthus micranthus Linn. parasitic onPersea americana

    Patience Ogoamaka Osadebe; Edwin Ogechukwu Omeje; Sylvester Chukwuemeka Nworu; Charles Okechukwu Esimone; Philip Felix Uzor; Ernest kenechukwu David; John Uchechukwu Uzoma

    2010-01-01

    Objective:To explore antidiabetic principles of the Eastern Nigeria mistletoe,Loranthus micranthus Linn. parasitic on Persea Americana.Methods:The weakly acidic fraction of the aqueous methanol extract of the leaves ofLoranthus micranthus (Linn.) was isolated and tested for its antidiabetic activities. The isolation of the weakly acidic fraction was carried out following established physico-chemical based procedures. Furthermore, alloxan-induced diabetic rats were treated intraperitoneally (ip) with250 mg/kg and400 mg/kg of the weakly acidic fraction, glibenclamide10mg/kg (positive control) and 2 mg/kg of3 % v/v tween20 (negative control). The sugar levels of the treated and untreated animals were determined by withdrawing the blood at regular intervals and testing them with an automated glucometer. The phytochemical analysis of the acidic fraction was carried out using standard procedures. Chromatographic techniques were employed in the subsequent isolation and purification of the constituents of the weakly acidic fraction.Results:It was shown that the maximum effect of the weakly acidic fraction was obtained at24 hours after administration and was found to be statistically comparable with that of the positive control. The phytochemical analysis revealed the presence of steroids, terpenoids, alkaloids, flavonoids, glycosides, carbohydrates, saponins, and acidic compounds in the crude extract and carbohydrates, flavonoids, terpenoids and oil in the weakly acidic fraction. Further purification of the weakly acidic fraction of the methanol extract using thin layer chromatography shows that toluene : methanol : diethyl amine (3:1:1) and chloroform: methanol: diethyl amine (9:1:1) are the best solvent system for the isolation of the various components of the weakly acidic fraction of the crude methanol extract ofLoranthus micranthus.Conclusions:The present study has led to the conclusion that the weakly acidic fraction of the plant under study has the potent

  11. Terpenoid variations within and among half-sibling avocado trees, Persea americana Mill. (Lauraceae.

    Jerome Niogret

    Full Text Available Chemical analyses were conducted to determine the qualitative and quantitative differences in monoterpenes and sesquiterpenes in plant material from avocado trees, Persea americana Mill. (Lauraceae. The initial study analyzed plant material sampled from the trunk to the leaves through different branch diameters to quantify proximo-distal spatial differences within a tree. All trees were seedlings initiated from a single maternal tree. Two-way analysis of variance was conducted on 34 chemicals that comprised at least 3% of the total chemical content of at least one tree and/or location within a tree. There were significant interactions between genotype and location sampled for most chemicals. Parentage analysis using microsatellite molecular markers (SSR's determined that the four trees had three fathers and that they represented two full-siblings and two half-sibling trees. Descriptive discriminant analysis found that both genotype and location within a tree could be separated based on chemical content, and that the chemical content from full-siblings tended to be more similar than chemical content from half-siblings. To further explore the relationship between genetic background and chemical content, samples were analyzed from leaf material from 20 trees that included two sets of full-sibling seedling trees, the maternal tree and the surviving paternal tree. Descriptive discriminant analysis found good separation between the two full-sibling groups, and that the separation was associated with chemistry of the parental trees. Six groups of chemicals were identified that explained the variation among the trees. We discuss the results in relation to the discrimination process used by wood-boring insects for site-selection on host trees, for tree selection among potential host trees, and the potential use of terpenoid chemical content in chemotaxonomy of avocado trees.

  12. Pistil starch reserves at anthesis correlate with final flower fate in avocado (Persea americana.

    María Librada Alcaraz

    Full Text Available A common observation in different plant species is a massive abscission of flowers and fruitlets even after adequate pollination, but little is known as to the reason for this drop. Previous research has shown the importance of nutritive reserves accumulated in the flower on fertilization success and initial fruit development but direct evidence has been elusive. Avocado (Persea americana is an extreme case of a species with a very low fruit to flower ratio. In this work, the implications of starch content in the avocado flower on the subsequent fruit set are explored. Firstly, starch content in individual ovaries was analysed from two populations of flowers with a different fruit set capacity showing that the flowers from the population that resulted in a higher percentage of fruit set contained significantly more starch. Secondly, in a different set of flowers, the style of each flower was excised one day after pollination, once the pollen tubes had reached the base of the style, and individually fixed for starch content analysis under the microscope once the fate of its corresponding ovary (that remained in the tree was known. A high variability in starch content in the style was found among flowers, with some flowers having starch content up to 1,000 times higher than others, and the flowers that successfully developed into fruits presented significantly higher starch content in the style at anthesis than those that abscised. The relationship between starch content in the ovary and the capacity of set of the flower together with the correlation found between the starch content in the style and the fate of the ovary support the hypothesis that the carbohydrate reserves accumulated in the flower at anthesis are related to subsequent abscission or retention of the developing fruit.

  13. Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.).

    Backer, Robert; Mahomed, Waheed; Reeksting, Bianca J; Engelbrecht, Juanita; Ibarra-Laclette, Enrique; van den Berg, Noëlani

    2015-01-01

    The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1) forms an integral part of the salicylic acid (SA) pathway in plants and is involved in cross-talk between the SA and jasmonic acid/ethylene (JA/ET) pathways. Therefore, NPR1 is essential to the effective response of plants to pathogens. Avocado (Persea americana) is a commercially important crop worldwide. Significant losses in production result from Phytophthora root rot, caused by the hemibiotroph, Phytophthora cinnamomi. This oomycete infects the feeder roots of avocado trees leading to an overall decline in health and eventual death. The interaction between avocado and P. cinnamomi is poorly understood and as such limited control strategies exist. Thus uncovering the role of NPR1 in avocado could provide novel insights into the avocado - P. cinnamomi interaction. A total of five NPR1-like sequences were identified. These sequences were annotated using FGENESH and a maximum-likelihood tree was constructed using 34 NPR1-like protein sequences from other plant species. The conserved protein domains and functional motifs of these sequences were predicted. Reverse transcription quantitative PCR was used to analyze the expression of the five NPR1-like sequences in the roots of avocado after treatment with salicylic and jasmonic acid, P. cinnamomi infection, across different tissues and in P. cinnamomi infected tolerant and susceptible rootstocks. Of the five NPR1-like sequences three have strong support for a defensive role while two are most likely involved in development. Significant differences in the expression profiles of these five NPR1-like genes were observed, assisting in functional classification. Understanding the interaction of avocado and P. cinnamomi is essential to developing new control strategies. This work enables further classification of these genes by means of functional annotation and is a crucial step in understanding the role of NPR1 during P. cinnamomi infection.

  14. Pistil starch reserves at anthesis correlate with final flower fate in avocado (Persea americana).

    Alcaraz, María Librada; Hormaza, José Ignacio; Rodrigo, Javier

    2013-01-01

    A common observation in different plant species is a massive abscission of flowers and fruitlets even after adequate pollination, but little is known as to the reason for this drop. Previous research has shown the importance of nutritive reserves accumulated in the flower on fertilization success and initial fruit development but direct evidence has been elusive. Avocado (Persea americana) is an extreme case of a species with a very low fruit to flower ratio. In this work, the implications of starch content in the avocado flower on the subsequent fruit set are explored. Firstly, starch content in individual ovaries was analysed from two populations of flowers with a different fruit set capacity showing that the flowers from the population that resulted in a higher percentage of fruit set contained significantly more starch. Secondly, in a different set of flowers, the style of each flower was excised one day after pollination, once the pollen tubes had reached the base of the style, and individually fixed for starch content analysis under the microscope once the fate of its corresponding ovary (that remained in the tree) was known. A high variability in starch content in the style was found among flowers, with some flowers having starch content up to 1,000 times higher than others, and the flowers that successfully developed into fruits presented significantly higher starch content in the style at anthesis than those that abscised. The relationship between starch content in the ovary and the capacity of set of the flower together with the correlation found between the starch content in the style and the fate of the ovary support the hypothesis that the carbohydrate reserves accumulated in the flower at anthesis are related to subsequent abscission or retention of the developing fruit.

  15. Phenolic compound profiles and antioxidant capacity of Persea americana Mill. peels and seeds of two varieties.

    Kosińska, Agnieszka; Karamać, Magdalena; Estrella, Isabel; Hernández, Teresa; Bartolomé, Begoña; Dykes, Gary A

    2012-05-09

    Avocado processing by the food and cosmetic industries yields a considerable amount of phenolic-rich byproduct such as peels and seeds. Utilization of these byproducts would be favorable from an economic point of view. Methanolic (80%) extracts obtained from lyophilized ground peels and seeds of avocado (Persea americana Mill.) of the Hass and Shepard varieties were characterized for their phenolic compound profiles using the HPLC-PAD technique. The structures of the identified compounds were subsequently unambiguously confirmed by ESI-MS. Compositional analysis revealed that the extracts contained four polyphenolic classes: flavanol monomers, proanthocyanidins, hydroxycinnamic acids, and flavonol glycosides. The presence of 3-O-caffeoylquinic acid, 3-O-p-coumaroylquinic acid, and procyanidin A trimers was identified in seeds of both varieties. Intervarietal differences were apparent in the phenolic compound profiles of peels. Peels of the Shepard variety were devoid of (+)-catechin and procyanidin dimers, which were present in the peels of the Hass variety. Peels of both varieties contained 5-O-caffeoylquinic acid and quercetin derivatives. The differences in the phenolic profiles between varietals were also apparent in the different antioxidant activity of the extracts. The peel extracts had a higher total phenolic compound content and antioxidant activity when compared to the seed extracts. The highest TEAC and ORAC values were apparent in peels of the Haas variety in which they amounted to 0.16 and 0.47 mmol Trolox/g DW, respectively. No significant (p > 0.05) differences were apparent between the TEAC values of seeds of the two varieties but the ORAC values differed significantly (p < 0.05). Overall these findings indicate that both the seeds and peel of avocado can be utilized as a functional food ingredient or as an antioxidant additive.

  16. A Novel Use for Potassium Alum as Controlling Agent Against Periplaneta americana (Dictyoptera: Blattidae).

    Salama, Elham M

    2015-12-01

    In this work, we present our results on the use of potassium alum as an environmentally friendly insecticide. This compound has the potential to rid our homes, schools, hotels, restaurants, and ships of cockroach infestations. This compound is environmentally friendly and has no hazardous effects on plant, animal, or human ecosystems. Alum was approved for medical use a long time ago. In our laboratory, we developed a novel method using potassium alum as an environmentally friendly insecticide to kill the most common cockroach in the subtropical region, Periplaneta americana (L.). Adult and nymph-staged cockroaches were left to feed on potassium alum per individual insect after a period of food deprivation. The mortality was recorded as LT50. The younger nymphs the third and early fourth instars died within 4 d of feeding after consuming an average of 0.3 mg per individual insect. Gravid females were highly susceptible to alum toxicity and experienced a higher mortality rate, with an average of 3 mg per individual female. The oothecae of the normal untreated females were 8.1 mm long and 4.13 mm wide and weighed 94 mg. The eggs laid by the treated gravid females were underweight and exhibited a dwarfism shape, and these eggs did not hatched if the females consumed the potassium alum before laying eggs. The results revealed that the adult male and female cockroaches have to consume 1 mg and 2.7 mg, respectively, of potassium alum to kill 100% of them after 1 month of ingestion. The potassium alum had to be ingested by the cockroaches to affect mortality. The effect of potassium alum was attributed to chronic toxicity and not acute toxicity. The potential applications of this novel technique will be discussed.

  17. Coccidian Parasites and Conservation Implications for the Endangered Whooping Crane (Grus americana.

    Miranda R Bertram

    Full Text Available While the population of endangered whooping cranes (Grus americana has grown from 15 individuals in 1941 to an estimated 304 birds today, the population growth is not sufficient to support a down-listing of the species to threatened status. The degree to which disease may be limiting the population growth of whooping cranes is unknown. One disease of potential concern is caused by two crane-associated Eimeria species: Eimeria gruis and E. reichenowi. Unlike most species of Eimeria, which are localized to the intestinal tract, these crane-associated species may multiply systemically and cause a potentially fatal disease. Using a non-invasive sampling approach, we assessed the prevalence and phenology of Eimeria oocysts in whooping crane fecal samples collected across two winter seasons (November 2012-April 2014 at the Aransas National Wildlife Refuge along the Texas Gulf coast. We also compared the ability of microscopy and PCR to detect Eimeria in fecal samples. Across both years, 26.5% (n = 328 of fecal samples were positive for Eimeria based on microscopy. Although the sensitivity of PCR for detecting Eimeria infections seemed to be less than that of microscopy in the first year of the study (8.9% vs. 29.3%, respectively, an improved DNA extraction protocol resulted in increased sensitivity of PCR relative to microscopy in the second year of the study (27.6% and 20.8%, respectively. The proportion of positive samples did not vary significantly between years or among sampling sites. The proportion of Eimeria positive fecal samples varied with date of collection, but there was no consistent pattern of parasite shedding between the two years. We demonstrate that non-invasive fecal collections combined with PCR and DNA sequencing techniques provides a useful tool for monitoring Eimeria infection in cranes. Understanding the epidemiology of coccidiosis is important for management efforts to increase population growth of the endangered whooping

  18. Pistil Starch Reserves at Anthesis Correlate with Final Flower Fate in Avocado (Persea americana)

    Alcaraz, María Librada; Hormaza, José Ignacio; Rodrigo, Javier

    2013-01-01

    A common observation in different plant species is a massive abscission of flowers and fruitlets even after adequate pollination, but little is known as to the reason for this drop. Previous research has shown the importance of nutritive reserves accumulated in the flower on fertilization success and initial fruit development but direct evidence has been elusive. Avocado (Persea americana) is an extreme case of a species with a very low fruit to flower ratio. In this work, the implications of starch content in the avocado flower on the subsequent fruit set are explored. Firstly, starch content in individual ovaries was analysed from two populations of flowers with a different fruit set capacity showing that the flowers from the population that resulted in a higher percentage of fruit set contained significantly more starch. Secondly, in a different set of flowers, the style of each flower was excised one day after pollination, once the pollen tubes had reached the base of the style, and individually fixed for starch content analysis under the microscope once the fate of its corresponding ovary (that remained in the tree) was known. A high variability in starch content in the style was found among flowers, with some flowers having starch content up to 1,000 times higher than others, and the flowers that successfully developed into fruits presented significantly higher starch content in the style at anthesis than those that abscised. The relationship between starch content in the ovary and the capacity of set of the flower together with the correlation found between the starch content in the style and the fate of the ovary support the hypothesis that the carbohydrate reserves accumulated in the flower at anthesis are related to subsequent abscission or retention of the developing fruit. PMID:24167627

  19. Foraging behavior of redheads (Aythya americana) wintering in Texas and Louisiana

    Woodin, M.C.; Michot, T.C.

    2006-01-01

    Redheads, Aythya americana, concentrate in large numbers annually in traditional wintering areas along the western and northern rim of the Gulf of Mexico. Two of these areas are the Laguna Madre of Texas and Chandeleur Sound of Louisiana. We collected data on 54,340 activities from 103 redhead flocks in Texas and 51,650 activities from 57 redhead flocks in Louisiana. Males and females fed similarly, differing neither in levels of feeding (percent of all birds in flock that were feeding) (p>0.90) nor in percentages of birds feeding by diving, tipping, dipping, or gleaning from the surface (p>0.10). The foraging level of redheads in the upper Laguna Madre region was relatively constant throughout two winters. Foraging of redheads in early winter in Louisiana was significantly greater than redhead foraging in the upper Laguna Madre, but by late winter, foraging by redheads in Louisiana had declined to the same level as that shown by redheads foraging in the upper Laguna Madre. The overall foraging level of redheads from Chandeleur Sound was greater (41%) than that of redheads in the upper Laguna Madre (26%), yet it was quite similar to the 46% foraging level reported for redheads from the lower Laguna Madre. Redheads in the upper Laguna Madre region of Texas fed more by diving than did those in the Chandeleur Sound and the lower Laguna Madre. Diving increased in frequency in late winter. Greater reliance by redheads on diving in January and February indicates that the birds altered their foraging to feed in deeper water, suggesting that the large concentrations of redheads staging at this time for spring migration may have displaced some birds to alternative foraging sites. Our results imply that the most likely period for food resources to become limiting for wintering redheads is when they are staging in late winter. ?? Springer 2006.

  20. Functional analysis of Scr during embryonic and post-embryonic development in the cockroach, Periplaneta americana.

    Hrycaj, Steven; Chesebro, John; Popadić, Aleksandar

    2010-05-01

    The cockroach, Periplaneta americana represents a basal insect lineage that undergoes the ancestral hemimetabolous mode of development. Here, we examine the embryonic and post-embryonic functions of the hox gene Scr in Periplaneta as a way of better understanding the roles of this gene in the evolution of insect body plans. During embryogenesis, Scr function is strictly limited to the head with no role in the prothorax. This indicates that the ancestral embryonic function of Scr was likely restricted to the head, and that the posterior expansion of expression in the T1 legs may have preceded any apparent gain of function during evolution. In addition, Scr plays a pivotal role in the formation of the dorsal ridge, a structure that separates the head and thorax in all insects. This is evidenced by the presence of a supernumerary segment that occurs between the labial and T1 segments of RNAiScr first nymphs and is attributed to an alteration in engrailed (en) expression. The fact that similar Scr phenotypes are observed in Tribolium but not in Drosophila or Oncopeltus reveals the presence of lineage-specific variation in the genetic architecture that controls the formation of the dorsal ridge. In direct contrast to the embryonic roles, Scr has no function in the head region during post-embryogenesis in Periplaneta, and instead, strictly acts to provide identity to the T1 segment. Furthermore, the strongest Periplaneta RNAiScr phenotypes develop ectopic wing-like tissue that originates from the posterior region of the prothoracic segment. This finding provides a novel insight into the current debate on the morphological origin of insect wings.

  1. Isolation and partial characterization of a protease from Agave americana variegata.

    Du Toit, P J

    1976-05-13

    A new protease was isolated from an extract of leaves of Agave americana variegata. The protease (EC 3.4.-) was purified 565-fold with a yield of 39.5%. The 43.8 mg enzyme had a specific activity of 0.44 units/mg. According to electrophoretic, ultracentrifugal and other physical characterizations the enzyme was homogeneous. The enzyme had a MR of 57000, a S20,W-value of 4.37 S, a D20, W-value of 6.8-7.0 - 10(-7) cm2sec-1, a Stokes radius of 3.18 nm, a partial specific volume of 0.735 cm3g-1, a frictional ration of 1.25, a molecular absorbancy index at 280 nm of 5.773-10(4), an isoelectric point of 5.25 and contained 8-10% carbohydrate. The enzyme contained no cysteine. Agave protease could hydrolyze a variety of protein substrates although it did have a restricted specificity. It is not a sulphhydryl protease but seems to be an alkaline "serine" protease with an optimum pH of 7.8-8.0 Agave protease had marked esterolytic activity and with Cbz-Tyr-ONp had an apparent Michaelis constant of 0.0345 -10(-3) M and a V of 1.24 mol substrate/mol enzyme per sec. The enzyme did not need metal ions for optimal activity, monovalent cations did not influence its kinetic parameters, but it was inhibited by cobalt, pC1HgBzO- and TosPheCH2C1. With respect to its primary specificity, as well as its pH-dependence there was a resemblance with chymotrypsin, although the rate of hydrolysis of Agave protease is much lower.

  2. Isolation and characterization of a cDNA encoding a membrane bound acyl-CoA binding protein from Agave americana L. epidermis.

    Guerrero, Consuelo; Martín-Rufián, M; Reina, José J; Heredia, Antonio

    2006-01-01

    A cDNA encoding an acyl-CoA binding protein (ACBP) homologue has been cloned from a cDNA library made from mRNA isolated from epidermis of young leaves of Agave americana L. The derived amino acid sequence reveals a protein corresponding to the membrane-associated form of ACBPs only previously described in Arabidopsis and rice. Northern blot analysis showed that the A. americana ACBP gene is mainly expressed in the epidermis of mature zone of the leaves. The epidermis of A. americana leaves have a well developed cuticle with the highest amounts of the cuticular components waxes, cutin and cutan suggesting a potential role of the protein in cuticle formation.

  3. A produção da "cidade latino-americana" The production of "the Latin American city"

    Adrián Gorelik

    2005-06-01

    Full Text Available Este trabalho propõe uma análise histórica da categoria "cidade latino-americana" como construção cultural. O argumento central é que, durante o período compreendido entre o segundo pós-guerra e a década de 1970, a idéia de "cidade latino-americana" funcionou como uma categoria do pensamento social, como uma figura do imaginário intelectual e político em amplas regiões do continente. Este texto procura reconstruir os principais itinerários conceituais e ideológicos da categoria, suas funções políticas e institucionais na conjuntura específica da região. Esse modo de entender a categoria "cidade latino-americana" em sua configuração histórica específica, sugere o autor, pode oferecer uma via produtiva para os estudos culturais latino-americanos.This paper suggests a historical analysis of the category "Latin American city" as a cultural construct. The central argument is that, during the period between the post-war and the 1970's, the idea of a Latin American city became a category of social thought, as part of the intellectual and political imagination in several regions of the continent. The text tries to rebuild the main conceptual and ideological turns, their political and institutional functions within the specific regional circumstances. The article suggests that this approach to the "Latin American city" category in its precise historical configuration may be a productive path for Latin-American cultural studies.

  4. Susceptibilidad a insectos en selecciones y variedades de annona muricata l. y persea americana m. en Puerto Rico

    Irma Cabrera

    2001-01-01

    Full Text Available En Puerto Rico se han reportado varios insectos en las guanábanas (Annona muricata L. y los aguacates (Persea americana M, sin embargo los de mayor importancia son el barrenador de la semilla Bephratelloides cubensis (A. y Apate monacha (F. en A. muricata y Pseudacysta persea (F. en P. americana. El objetivo principal de esta investigación fue determinar la incidencia de B. cubensis y A. monacha en seis selecciones de guanábana y en siete variedades de aguacate. De las evaluaciones realizadas en A. muricata, se observó que las selecciones IV-10, VII-14 y VI-1 mostraron la mayor incidencia y la IV-16 mostró la menor incidencia de B. cubensis en relación al número de barrenos por fruta. En cuanto a la incidencia de A. monacha en A. muricata las selecciones IV-16, IV-2, IV-3, VII-14 presentaron la mayor incidencia de barrenos por árbol. En las evaluaciones realizadas en Persea americana se seleccionaron las variedades Ávila, Semil 34, Wilson Popenoe, Pollock, Candelaria, Semil 43 y Butler como las variedades comerciales más sembradas en Puerto Rico. Se observó que la variedad Avila obtuvo el mayor número de huevos, ninfas, hembras y machos de P. persea por hojas comparadas con las demás variedades. Respecto al número de hojas infestadas por árbol se encontró valores significativos (P≤0,01 en la variedad Avila. Resultando tener un 90% de las hojas dañadas. No se observó que las variedades evaluadas tuviese baja o ninguna preferencia a P. Persea

  5. O novo na sociologia latino-americana Novelty in Latin American sociology

    Lucio Oliver Costilla

    2005-12-01

    Full Text Available O artigo analisa varias caraterísticas atuais das ciências sociais latino-americanas em paralelo ao desenvolvimento da América Latina nos últimos anos. O ponto de partida é o sinal de que a combinação contraditória no continente latino-americano entre uma intelectualidade que tem um elevado nível cultural e a existência de movimentos sociais e políticos numa situação social explosiva, gera amplas possibilidades para o desenvolvimento da sociologia. A crise das ciências sociais acompanha estas mudanças significativas, na busca de novas perspectivas analíticas capazes de explicar o ritmo de mudanças contemporâneas. A rigor, no artigo, tenta-se demonstrar que o que a sociologia latino-americana tem como novo é um regresso ao pensamento crítico que a caracterizou no passado. Uma das mais importantes heranças da história sociológica latino-americana é o estudo e a classificação, desde uma perspectiva de totalidade, das estruturas sociais e dos processos sociopolíticos como fundamento para analisar o posicionamento dos atores e a dinâmica das instituições sociais. Assim, abre-se espaço para uma saudável tendência nas ciências sociais: superar o empirismo, reencontrando-se com a teoria. Uma necessidade fundante, hoje, para a América Latina é atualizar a sua procura de desenvolvimento: como estar à altura do mundo, como universalizar-se para viver o momento histórico atual com todas as potencialidades sociais possíveis, superando formas de produção e de vida atrasadas e caducas. E mais: como co-participar na crítica das novas formas modernas e pós-modernas, vislumbrando novos modelos de civilização, fazendo a crítica da democracia liberal e dos Estados nacionais de concorrência de latino-americanos, e impulsionando a recuperacão do público democrático perante a tendência ao autoritarismo estatal. Os aportes que a América Latina pode dar à crítica e à renovação da democracia política, à reforma

  6. Localização nervosa da blastomicose sul-americana

    Açucena Raphael

    1966-06-01

    Full Text Available Foram estudados sob os pontos de vista clínico-neurológico e liquorológico, 55 doentes que apresentavam formas variadas, tegumentares e viscerais, da blastomicose sul-americana; no seguimento, 46 dêsses casos foram submetidos a nôvo exame clínico-neurológico e 30 foram repuncionados para novos exames de LCR. Dos 55 pacientes estudados, 14 apresentavam sintomas ou sinais clínicos sugestivos de comprometimento do SNC e/ou de seus envoltórios; em 5 casos deste último grupo o exame do líquido cefalorraqueano forneceu dados que permitiam o diagnóstico etiológico. Dos 41 doentes que não apresentavam sinais ou alterações neurológicas, em um o exame do LCR forneceu dados de valor diagnóstico, inclusive a positividade da pesquisa micológica. Vinte e três doentes foram considerados normais do ponto de vista neurológico e 26 mostraram alterações clínicas e liqüóricas ocasionais e duvidosas quanto ao seu valor diagnóstico. De acôrdo com os resultados obtidos pelo exame do LCR, os doentes foram distribuídos em 3 grupos: 1 doentes com LCR normal; 2 doentes cujo LCR apresentava um conjunto de alterações atribuíveis à paracoccidioidose; 3 doentes cujo LCR apresentava alterações classificáveis como ocasionais ou duvidosas quanto ao seu valor para o diagnóstico etiológico. As alterações do LCR consideradas como sugestivas quanto ao diagnóstico etiológico consistiram em: a modificações quantitativas e qualitativas das frações protêicas, sendo mais freqüente o aumento do teor de gama-globulina; b positividade da reação de fixação do complemento com título acima de 3; c pleocitose, intensa e do tipo misto mas com predominância de linfócitos nos casos em que havia acometimento leptomeníngeo, discreta e de tipo linfomononuclear nos casos de provável localização parenquimatosa; d hipoglicorraquia que ocorreu apenas nos casos de localização leptomeníngea; e positividade do exame micológico. A presença de

  7. Characterization of water and sediment quality at the Americana reservoir related to the occurrence of aquatic plants

    E.D. Velini; E. Negrisoli; A.L. Cavenaghi; M.R. Corrêa; Bravin, L.F.N.; Marchi, S.R.; M.L.B. Trindade; Arruda,D.P.; Padilha, F.S.

    2005-01-01

    Este trabalho foi realizado na UHE Americana, pertencente à Companhia Paulista de Força e Luz, e faz parte de um projeto de pesquisa e desenvolvimento realizado em conjunto com a Faculdade de Ciências Agronômicas (UNESP) de Botucatu. Foram realizadas amostragens de água e sedimento nos meses de outubro e dezembro de 2003 e fevereiro, abril e junho de 2004. Selecionaram-se seis pontos de coleta no reservatório, sendo cinco a montante da barragem e um a jusante. Levantamentos de flora foram rea...

  8. Chemical Composition, Functional Properties, and Effect of Inulin from Tunisian Agave americana L. Leaves on Textural Qualities of Pectin Gel

    Bouaziz, Mohamed Ali; Rassaoui, Rabaa; Besbes, Souhail

    2014-01-01

    In this study, the chemical composition and functional properties of Agave americana L. (AA) leaves were determined. The Agave leaves powder had a high amount of total dietary fiber (38.40%), total sugars (45.83%), and protein (35.33%), with a relatively low content in ash (5.94%) and lipid (2.03%). The Agave leaves were exhibited with potential food application. The Agave inulin showed a significant difference compared with the commercial inulin as for aw (0.275 against 0.282), pH (5.53 agai...

  9. O romance como coletânea de contos: a redução nas literaturas contemporâneas latino-americanas

    MÜLLER, Gesine

    2016-01-01

    Resumo O romance como coletânea de contos experimenta um notável vigor nas literaturas latino-americanas aproximadamente desde 1989. Isto possivelmente se deve, entre outros fatores, à mudança de paradigma realizada em diversos graus pelos próprios autores do chamado boom da literatura latino-americana. O presente artigo compara as primeiras obras de representantes canônicos como Carlos Fuentes e Gabriel García Márquez com as respectivas obras tardias, observando em que medida ocorreu uma mud...

  10. Acute and sub-acute toxicological assessment of the aqueous seed extract of Persea americana mill (Lauraceae) in rats.

    Ozolua, Raymond I; Anaka, Ogochukwu N; Okpo, Stephen O; Idogun, Sylvester E

    2009-07-03

    The aqueous seed extract of Persea americana Mill (Lauraceae) is used by herbalists in Nigeria for the management of hypertension. As part of our on-going scientific evaluation of the extract, we designed the present study to assess its acute and sub-acute toxicity profiles in rats. Experiments were conducted to determine the oral median lethal dose (LD(50)) and other gross toxicological manifestations on acute basis. In the sub-acute experiments, the animals were administered 2.5 g/kg (p.o) per day of the extract for 28 consecutive days. Animal weight and fluid intake were recorded during the 28 days period. Terminally, kidneys, hearts, blood/sera were obtained for weight, haematological and biochemical markers of toxicity. Results show that the LD(50) could not be determined after a maximum dose of 10 g/kg. Sub-acute treatment with the extract neither affected whole body weight nor organ-to-body weight ratios but significantly increased the fluid intake (P americana is safe on sub-acute basis but extremely high doses may not be advisable.

  11. Cardiotoxicity of acetogenins from Persea americana occurs through the mitochondrial permeability transition pore and caspase-dependent apoptosis pathways.

    Silva-Platas, Christian; García, Noemí; Fernández-Sada, Evaristo; Dávila, Daniel; Hernández-Brenes, Carmen; Rodríguez, Dariana; García-Rivas, Gerardo

    2012-08-01

    Acetogenins are cell-membrane permeable, naturally occurring secondary metabolites of plants such as Annonaceae, Lauraceae and other related phylogenic families. They belong to the chemical derivatives of polyketides, which are synthesized from fatty acid precursors. Although acetogenins have displayed diverse biological activities, the anti-proliferative effect on human cancer cells has been widely reported. Acetogenins are inhibitors of complex I in the electron transport chain therefore they interrupt ATP synthesis in mitochondria. We tested a new acetogenins-enriched extract from the seed of Persea americana in order to investigate if any toxicity was induced on cardiac tissue and determine the involved mechanism. In isolated perfused heart we found that contractility was completely inhibited at an accumulative dose of 77 μg/ml. In isolated cardiomyocytes, the acetogenins-enriched extract induced apoptosis through the activation of the intrinsic pathway at 43 μg/ml. In isolated mitochondria, it inhibited complex I activity on NADH-linked respiration, as would be expected, but also induced permeability transition on succinate-linked respiration. Cyclosporine A, a known blocker of permeability transition, significantly prevented the permeability transition triggered by the acetogenins-enriched extract. In addition, our acetogenins-enriched extract inhibited ADP/ATP exchange, suggesting that an important element in phosphate or adenylate transport was affected. In this manner we suggest that acetogenins-enriched extract from Persea americana could directly modulate permeability transition, an entity not yet associated with the acetogenins' direct effects, resulting in cardiotoxicity.

  12. Composition and ultrastructure of the suberized cell wall of isolated crystal idioblasts from Agave americana L. leaves.

    Espelie, K E; Wattendorff, J; Kolattukudy, P E

    1982-07-01

    Styloid-calcium-oxalate-crystal-containing idioblasts possess an interior cell-wall layer which has a lamellar ultrastructure. Idioblasts were isolated by centrifugation of an Agave americana leaf homogenate through 2M sucrose. The aliphatic monomers of the polymeric material from an idioblast fraction were primarily ω-hydroxy acids (32%) and dicarboxylic acids (35%), with C18:1 dicarboxylic acid being the most dominant monomer (25%). Nitrobenzene oxidation of the idioblasts yielded syringaldehyde and vanillin in a ratio of 0.46:1. The major class of wax associated with the idioblasts was free fatty acids (34%). A major homologue of both the fatty acid and fatty alcohol fractions of this wax was C22. The hydrocarbon fraction of the wax had a broad chainlength distribution with a large amount of even-numbered (47%) and shorter-chain homologues. The ultrastructure, the composition of the aliphatic and aromatic components of the polymeric material as well as the composition of the wax show that the idioblast cell wall is suberized. The wax and cutin polymer of the epidermis of A. americana leaves were chemically characterized for comparative purposes.

  13. In vivo effects of ecdysterone on puff formation, and RNA and protein synthesis in the salivary glands of Rhynchosciara americana.

    Alvarenga, C A; Winter, C E; Stocker, A J; Pueyo, M T; Lara, F J

    1991-01-01

    1. Fourth-instar larvae of Rhynchosciara americana were injected with the insect molting hormone, ecdysterone, giving final hemolymph concentrations from 4.46 to 223 microM. 2. Induction of the DNA puff, B2b, in the proximal (S1) region of the salivary glands of Rhynchosciara americana by 22.6 microM ecdysterone, was accompanied by the production of an mRNA and a polypeptide with the same characteristics as B2b products produced during normal development. This mRNA and polypeptide were restricted to the proximal region of the gland, as is the B2b puff. 3. Synthesis of other poly(A)+RNAs was also stimulated in S1 by ecdysterone, and other puffs that appear during normal development were induced. However, rRNA production in S1 goes through a pattern of inhibition, followed by recovery when B2b is puffed, and subsequent inhibition. 4. Low molecular weight RNA, with a peak in the region of 4S, is stimulated after ecdysterone administration.

  14. Aprisionamento e libertação: duas Antígonas latino-americanas

    Eduardo Guerreiro Brito Losso

    2014-09-01

    Full Text Available O artigo apresenta uma análise estrutural comparativa de duas peças teatrais latino-americanas que reatualizam a Antígona de Sófocles: Antígona Vélez (1951, de Leopoldo Marechal (Argentina, e La pasión según Antígona Pérez (1968, de Luis Rafael Sánchez (Porto Rico. A partir de dados formais, o artigo reflete sobre as condições do confronto do indivíduo com os novos tipos de tiranias modernas. Antígona não é só um mártir ficcional: é um exemplo de como encontrar espaço para o desenvolvimento da autonomia individual.Abstract: The paper presents a comparative structural analysis of two Latin American plays that update Sophocles’ Antigone: Antígona Vélez (1951: Leopoldo Marechal (Argentina and La pasión según Antígona Pérez (1968, Luis Rafael Sánchez (Puerto Rico. From formal data, the paper reflects on the conditions of confrontation of the individual with new types of modern tyrannies. Antigone is not only a fictional martyr: is an example of how to find space for the development of individual autonomy.Keywords: Antigone; myth and modernity; autonomy; structural analysis; Latin American theatre Eduardo Guerreiro Brito Losso é professor adjunto de Teoria Literária do departamento de Ciência da Literatura da UFRJ desde 2014, tendo ocupado o mesmo cargo na UFRuralRJ de 2009 a 2014. Seus temas de pesquisa são: a elaboração de modos de viver na poesia e na filosofia, a secularização da mística na literatura moderna e a questão do valor estético no mercado da música pop. Trabalha a partir da Teoria Crítica a crítica da indústria cultural e relações entre estética e religião. Fez parte da organização dos livros “Diferencia minoritaria en Latinoamérica”, pela Georg Olms, 2008, e “O carnaval carioca de Mario de Andrade”, Azougue, de 2011.

  15. Linfadenopatias na leishmaniose tegumentar americana: considerações sobre dois casos

    Mário A. P. Moraes

    1993-09-01

    Full Text Available Em dois pacientes, com suspeita clínica de linfoma ou escrofuloderma - por apresentarem linfadenopatias cervicais importantes o estudo dos linfonodos removidos para exame histológico revelou um processo necrótico-granulomatoso e a presença de leishmanias (amastigotas no interior de macrófagos. Lesões cutâneas ou em membranas mucosas, características da leishmaniose tegumentar americana, não foram percebidas, embora um dos pacientes viesse posteriormente a desenvolvê-las, como provável efeito de tratamento para toxoplasmose. O achado fundamenta a suspeita de que os agentes da doença, após penetrarem no organismo do hospedeiro, podem alojar-se em órgãos do sistema monocítico-fagocitário, aí permanecendo durante muito tempo, talvez mesmo por toda a vida do indivíduo infectado. Eventualmente, sob a ação de fatores diversos, capazes de afetar a resistência do hospedeiro, migrariam os parasitos para a pele ou as mucosas, determinando lesões secundárias ou de reativação.Two patients, with cervical lymphadenopathies and a presumptive diagnosis of lymphoma or scrofula, were submitted to biopsies of the affected lymph nodes. Unexpectedfy, the histological picture revealed anecrotic- granulomatous reaction and the presence of leishmania (amastigotes in some vacuolated macrophages. The patients werefrom different endemic areas of leishmaniasis in Brazil, and had no perceptible cutaneous or mucosal lesions. Later, however, one of them developed such lesions, probably as an effect of a treatment for toxoplasmosis. These findings support the idea that the agents of the disease, once in the host organism, would invade the organs of the phagocitic-mononuclear system, there remaining for a long time, maybe for the rest of the host life. Eventualfy, under the action of several factors, that could modify the host resistance, the parasites migrate to the skin or the mucosal membranes, causing secondary or reactivating lesions.

  16. The rhizome of Reclinomonas americana, Homo sapiens, Pediculus humanus and Saccharomyces cerevisiae mitochondria

    Raoult Didier

    2011-10-01

    Full Text Available Abstract Background Mitochondria are thought to have evolved from eubacteria-like endosymbionts; however, the origin of the mitochondrion remains a subject of debate. In this study, we investigated the phenomenon of chimerism in mitochondria to shed light on the origin of these organelles by determining which species played a role in their formation. We used the mitochondria of four distinct organisms, Reclinomonas americana, Homo sapiens, Saccharomyces cerevisiae and multichromosome Pediculus humanus, and attempted to identify the origin of each mitochondrial gene. Results Our results suggest that the origin of mitochondrial genes is not limited to the Rickettsiales and that the creation of these genes did not occur in a single event, but through multiple successive events. Some of these events are very old and were followed by events that are more recent and occurred through the addition of elements originating from current species. The points in time that the elements were added and the parental species of each gene in the mitochondrial genome are different to the individual species. These data constitute strong evidence that mitochondria do not have a single common ancestor but likely have numerous ancestors, including proto-Rickettsiales, proto-Rhizobiales and proto-Alphaproteobacteria, as well as current alphaproteobacterial species. The analysis of the multichromosome P. humanus mitochondrion supports this mechanism. Conclusions The most plausible scenario of the origin of the mitochondrion is that ancestors of Rickettsiales and Rhizobiales merged in a proto-eukaryotic cell approximately one billion years ago. The fusion of the Rickettsiales and Rhizobiales cells was followed by gene loss, genomic rearrangements and the addition of alphaproteobacterial elements through ancient and more recent recombination events. Each gene of each of the four studied mitochondria has a different origin, while in some cases, multichromosomes may allow for

  17. The effects of captive rearing on the behavior of newly-released whooping cranes (Grus americana)

    Kreger, M.D.; Hatfield, J.S.; Estevez, I.; Gee, G.F.; Clugston, D.A.

    2005-01-01

    Rearing treatments used in captivity to prepare animals for reintroduction to the wild may have a profound effect on behavior and, possibly, affect their survival after reintroduction. This study examined the behaviors of captive-reared whooping cranes (Grus americana) upon their release in Florida to determine if rearing treatments may affect the behavior of the birds and how these affect their chances of survival in the wild. Individually tagged birds were observed at the rearing facility, the U.S. Geological Survey Patuxent Wildlife Research Center in Maryland, from hatch to 20 weeks of age and at the release site in Central Florida for up to 6 weeks post release. The rearing treatments were parent reared (PR), hand reared (HR), and hand reared with exercise (HRE). Observations at the rearing facility are described in a previous paper. At the release site, each bird was observed for 5 min every morning (0700?1000 h) and late afternoon (1500?1800 h) during the 6-week study period. Our results indicated that most of the time, the n = 34 birds were foraging (46.03 ? 1.48%), followed by nonvigilant (20.89 ? 0.73%), vigilant (19.21 ? 0.72%), or performing comfort behaviors (11.61 ? 1.28%). Data were analyzed using mixed models repeated measures ANOVA. There were no significant behavioral differences between HR and HRE birds. PR birds were found in larger groups than HR birds during the first 2 weeks post release and greater than HR and HRE birds afterwards. This may be interpreted as an antipredator strategy for birds that relied on parental guidance during rearing. HR and HRE birds foraged more than PR birds during the first 2 weeks post release and PR birds were more vigilant during the first 2 weeks post release. Across rearing treatments, the percentages of time spent foraging and engaged in vigilant behaviors during rearing were positively correlated with their behavior upon release. If any of these behaviors can be demonstrated to have relevance for the

  18. Caracterização física e química de frutos de abacate visando a seu potencial para extração de óleo Physical and chemical characterization of avocado fruits aiming its potencial for oil extraction

    João Shojiro Tango

    2004-04-01

    Full Text Available Vinte e quatro variedades de abacate provenientes da coleção de abacateiros, situada no Núcleo Experimental de Campinas e pertencente ao Centro de Fruticultura do Instituto Agronômico de Campinas (IAC, foram analisadas quanto às proporções dos componentes dos frutos (polpa, casca e caroço e quanto aos teores de lipídeos e umidade da polpa fresca. Determinaram-se, na maioria das variedades, o perfil de ácidos graxos dos óleos extraídos da polpa e, em algumas das variedades, a composição química dos caroços dos frutos. As variedades mais indicadas para serem cultivadas, visando à utilização industrial dos frutos para a extração de óleo, baseando-se nos teores de lipídeos encontrados na polpa fresca e estabelecidos acima de 18%, foram: Anaheim, Carlsbad, Collinson, Fuerte, Glória, Hass, Itzamna, Mayapan, Ouro Verde e Wagner. O período de colheita dessas variedades estendeu-se por sete meses, iniciando em maio e terminando em novembro. Houve correlações lineares negativas, altamente significativas, entre as proporções de polpa com caroço e casca dos frutos, entre os teores de umidade e lipídeos nas polpas e entre os teores de umidade e amido nos caroços dos frutos. Ocorreram grandes variações na composição de ácidos graxos constituintes do óleo das polpas e na composição química das sementes.Twenty-four avocado varieties from the germplasm collection of the Fruit Center of the Agronomic Institute of Campinas, were evaluated as a possible raw material for oil extraction. The varieties were analyzed concerning the proportions of fruit components (pulp, peel and seed and the amounts of moisture and fatty substances found in the fresh pulp. The fatty acid profile of the oils extracted from the pulp was determined in most of the varieties, and in some varieties also the fruit seeds chemical composition. The Anaheim, Carlsbad, Collinson, Fuerte, Glória, Hass, Itzamna, Mayapan, Ouro Verde and Wagner fruit varieties

  19. O papel da tradução na construção da identidade da literatura afro-americana no Brasil

    Lauro Maia AMORIM

    2013-10-01

    Full Text Available Este artigo discute o papel da tradução na construção da identidade da literatura afro-americana no Brasil, levando em consideração as relações entre o contexto sociocultural brasileiro, demarcado pela mestiçagem tanto biológica quanto cultural, e o modo peculiar com que a perspectiva da crítica literária representada pelos ensaios e traduções do crítico brasileiro Sérgio Milliet, produzidos entre as décadas de 40 e 60, aborda a poética afro-americana, com foco especial na poesia de Langston Hughes. No artigo discutem-se as diferenças entre os contextos brasileiro e norte-americano em relação aos discursos da miscigenação e da raça. Analisa-se em que medida Sérgio Milliet formulou uma identidade racializada para a poesia afro-americana em seus ensaios, reconstruindo-a, porém, por meio da tradução, na sua antologia Obras Primas da Poesia Universal, de forma menos racializada e de modo que a estética afro-americana pudesse soar menos dissonante e regional, e mais aliada ao princípio da universalidade que caracteriza a antologia, composta por renomados poetas brasileiros e estrangeiros.

  20. Remnant B-cell-stimulative and anti-oxidative effects of Persea americana fruit extract studied in rats introduced into streptozotocin - induced hyperglycaemic state.

    Rao, U S Mahadeva; Adinew, Bizuneh

    2011-01-01

    Insulin-stimulative and anti-oxidative effects of Persea americana fruit extract were evaluated using streptozotocin (STZ). Ethanol extract of P. americana in the concentration of 300 mg/kg body weight/rat /day was orally administered to rats introduced into STZ-induced hyperglycaemic state for a period of 30 days. After the treatment with avocado fruit extract, the elevated levels of blood glucose, glycosylated haemoglobin, blood urea and serum creatinine seen in the hyperglycaemic rats, reverted back to near normal. Similarly, significantly decreased plasma insulin and haemoglobin levels went back to near normal after the treatment, suggesting the insulin-stimulative effect of P. americana fruit. Determination of thiobarbituric acid reactive substances (TBARS), hydroperoxides and both enzymatic and non-enzymatic antioxidants, confirmed the anti-oxidative potential of avocado fruit extract which, in turn, might be responsible for its hypoglycaemic potential. Changes in activities of enzymes such as serum aspartate transaminase (AST), serum alanine transaminase (ALT), and serum alkaline phosphatase (ALP) seen in the control and experimental rats, revealed the tissue-protective nature of Persea americana fruits, while all of the analysed biochemical parameters were comparable to those obtained with gliclazide as a standard reference drug.

  1. Abundance in Persea americana of the Redbay Ambrosia Beetle, Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae), Vector of Laurel Wilt: A Case of Intra-guild Competition?

    The redbay ambrosia beetle, Xyleborus glabratus is a pest of plant species in the Lauraceae, including Persea borbonia, P. pallustris, P. americana, and others. Xyleborus glabratus infestation levels in P. borbonia maintain a high proportion compared to other species, such as Xylosandrus crassiuscu...

  2. EFICÁCIA DA FONOFORESE COM XIMENIA AMERICANA L. NA INFLAMAÇÃO DE TENDÃO DE RATOS

    Seânia Santos Leal

    Full Text Available RESUMO Introdução: Diversos recursos terapêuticos, como laser e ultrassom isolado, combinados com fármacos e fonoforese têm sido utilizados em casos de inflamação e reparo de tendão, sendo o ultrassom pulsado bastante eficaz em tendinites crônicas. Objetivo: Analisar a eficácia do uso da fonoforese com o extrato etanólico das cascas do caule da Ximenia americana L. na resolução do processo inflamatório crônico em Rattus norvegicus. Métodos: A amostra consistiu em 120 animais para análise da resposta inflamatória, utilizando-se como variáveis edema, força biomecânica, número de fibroblastos e análise histológica. Resultados: Nos 7° e no 14° dia, verificou-se diferença significativa entre o grupo ultrassom com gel do caule de Ximenia americana L. e o grupo ultrassom com placebo (p < 0,05 quanto à redução de edema, aumento da força máxima de ruptura, redução extremamente significativa da deformação máxima (p < 0,001, além de aumento dos fibroblastos. Na análise histológica, houve melhora do processo inflamatório inicial e aceleração do reparo tendíneo, com redução de células inflamatórias e com deposição de colágeno organizado com matriz extracelular densa. Conclusão: O ultrassom pulsado combinado com o gel do caule de Ximenia americana L. é uma forma terapêutica eficaz para a resolução do processo inflamatório crônico.

  3. Immunotherapy, immunochemotherapy and chemotherapy for American cutaneous leishmaniasis treatment Imunoterapia, imunoquimioterapia e quimioterapia no tratamento da leishmaniose tegumentar americana

    Wilson Mayrink

    2006-02-01

    Full Text Available The first choice of treatment for American cutaneous leishmaniasis is the pentavalent antimonial drug. Although it has been shown that this treatment is mostly effective and indicated, some disadvantages should be taken into account such as side effects, long term treatment inconveniences and counter-indication for patients suffering from cardiopathy, nephropathy; yet, aging, pregnancy and other conditions. With the advent of the vaccine anti-American cutaneous leishmaniasis as a prophylactic measure, studies on therapy using the vaccine associated or not with other drugs have been performed by many investigators and it is currently among the alternative treatments and prevention measures for American cutaneous leishmaniasis. In conclusion, the association between antimony and vaccine (immunochemotherapy showed the same cure rate when compared with the standard treatment (100% and it was also able to reduce the salt volume in 17.9% and treatment length from 87 to 62 days, decreasing side effects.O tratamento de primeira escolha para leishmaniose tegumentar americana é o antimonial pentavalente. Embora este tratamento seja na maioria das vezes efetivo e indicado, devem ser consideradas as desvantagens tais como efeitos colaterais, longa duração do tratamento e contra-indicação para cardiopatas, nefropatas, idosos, grávidas e outras condições. Com o advento da vacina antileishmaniose tegumentar americana para fins profiláticos e terapêuticos, associando-a ou não a outros fármacos, muitas pesquisas têm sido desenvolvidas, sendo a vacina a principal entre os atuais recursos no tratamento e prevenção da leishmaniose tegumentar americana. Em conclusão, a associação do antimônio com a vacina (imunoquimioterapia apresentou o mesmo índice de cura em relação ao tratamento padrão (100%, e ainda reduziu o volume do sal em 17,9% e o tempo de cura significativamente, de 87 para 62 dias; conseqüentemente, reduzindo os efeitos colaterais.

  4. CARACTERÍSTICAS DO FRUTO DA VARIEDADE SPAN AMERICANA (Citrus reticulata Blanco: UMA TANGERINA DO TIPO 'PONCÃ' DE MATURAÇÃO PRECOCE FRUIT CHARACTERISTICS OF THE SPAN AMERICANA (Citrus reticulata Blanco: AN EARLY RIPENING 'PONKAN' LIKE MANDARIN

    ROSE MARY PIO

    2001-08-01

    Full Text Available A tangerina-'Poncã' é bastante apreciada pelo consumidor brasileiro. No Estado de São Paulo, a maturação de seus frutos ocorre nos meses de abril a agosto. Com o mercado ávido por frutas de mesa e agravado pelo problema da ocorrência da clorose variegada dos citros (CVC, que afeta principalmente as variedades de laranjas-doces, tem havido um aumento considerável no plantio dessa variedade. Dessa forma, um excesso de produção, num mesmo período, faz com que os preços caiam, desestimulando assim os produtores. O Centro de Citricultura Sylvio Moreira (CCSM vem buscando outras alternativas no sentido de obter, durante o ano todo, esse tipo de tangerina. Assim sendo, foram realizados estudos de caracterização de frutos, em diversas épocas do ano, com o acompanhamento dos níveis de maturação, envolvendo acessos do Banco de Germoplasma do CCSM. Dentre as variedades estudadas, quanto à precocidade e qualidade dos frutos, destacou-se a Span Americana. A tangerina-'Poncã' tem seu período de maturação para as condições edafoclimáticas do CCSM, nos meses de maio e junho. Já a variedade Span Americana, que apresenta frutos similares à 'Poncã' tradicional, tem maturação bastante precoce, podendo ser iniciada a sua colheita no mês de março.The 'Ponkan' mandarin is highly appreciated by the Brazilian consumer. In the State of São Paulo, Brazil, its fruits mature from April through August. With the market very much in demand for table fruits and due to the problem of occurrence of citrus variegated chlorosis (CVC that affects mainly the sweet orange varieties there has been a considerable increase in planting of this variety. By this way, an over of production in a same period makes the prices drop, desistimulating the growers. The Centro de Citricultura Sylvio Moreira (CCSM is searching other alternatives in order to obtain mandarin fruits all the year. Therefore, fruit characteristics studies in different seasons of the year

  5. Sustitución de lardo por grasa vegetal en salchichas: incorporación de pasta de aguacate. Efecto de la inhibición del oscurecimiento enzimático sobre el color Substituição do toucinho por gordura vegetal em salsichas: adição da pasta de abacate. Efeito da inibição do escurecimiento enzimatico na cor

    Ubaldo Rueda-Lugo

    2006-06-01

    Full Text Available La sustitución de grasa animal es importante para mejorar la calidad nutricional de los alimentos de origen animal. Tradicionalmente, los embutidos contienen cantidades relativamente altas de grasas insaturadas, por lo que se ha buscado la sustitución parcial o total de estas con grasas o aceites de origen vegetal. El aprovechamiento del aguacate como fuente de grasa vegetal es una alternativa para este tipo de productos. La oxidación de este fruto es uno de los principales problemas durante la industrialización. En este trabajo se han añadido dos inhibidores del oscurecimiento enzimático, ácido ascórbico y eritorbato de sodio, para determinar el efecto de éstos sobre el color de salchichas de cerdo. Se han encontrado diferencias en la luminosidad de las muestras con respecto al tratamiento control, además de que este mismo parámetro se ha reducido con el tiempo de almacenamiento. No ha habido efecto significativo de los antioxidantes sobre las otras componentes del color. El uso de eritorbato de sodio en la elaboración de pasta de aguacate ha reducido el oscurecimiento de la pasta durante su congelación, sin afectar mayormente otros parámetros de color del producto terminado. La aceptación del producto por un grupo de consumidores ha sido aceptable.A substituição da gordura animal ou toucinho é importante para melhorar a qualidade nutricional dos alimentos de origem animal. Tradicionalmente, os embutidos contêm quantidades relativamente elevadas de gorduras insaturadas, e a substituição parcial ou total com gordura vegetal ou óleo é desejável. O abacate é fonte de gordura vegetal como uma alternativa para este tipo de produto. A oxidação desta fruta é um dos problemas principais durante a industrialização. Neste trabalho foram adicionados dois inibidores de escurecimento enzimatico, ácido ascórbico e eritorbato do sódio, para determinar o efeito destes na cor das salsichas. As diferenças na luminosidade das

  6. [The Unión Médica Hispano-Americana (1900) and its contribution to scientific internationalism].

    Olagüe de Ros, Guillermo

    2006-01-01

    The Unión Médica Hispano-Americana was founded during the XIII International Congress of Medicine of Paris in 1900. Its main aim was to strengthen the cultural and scientific ties among Spanish speaking countries. The first assembly took place in Madrid in the Spring of 1903. The most interesting paper presented was undoubtedly that of Manuel Tolosa Latour. He suggested creating a Diccionario Tecnológico Médico Hispano-Americano. The society had no continuity and neither did the dictionary. The reasons for this failure must be seen in the absence of economic support by political authorities in Spain and America, the organizational weakness of the Unión and the poverty of its action program, which was imbued with a triumphalist rhetoric, empty of content and highly paternalistic in its relations with the American world.

  7. Female gonadal hormones and reproductive behaviors as key determinants of successful reproductive output of breeding whooping cranes (Grus americana)

    Brown, Megan E; Converse, Sarah J.; Chandler, Jane N.; Shafer, Charles; Brown, Janine L; Keefer, Carol L; Songsasen, Nucharin

    2016-01-01

    Reproductive success of endangered whooping cranes (Grus americana) maintained ex situ is poor. As part of an effort to identify potential causes of poor reproductive success in a captive colony, we used non-invasive endocrine monitoring to assess gonadal and adrenal steroids of bird pairs with various reproductive outcomes and evaluated the relationships of hormones and behaviors to reproductive performance. Overall, reproductively successful (i.e., egg laying) females had significantly higher mean estrogen levels but lower mean progestogen concentrations than did unsuccessful females. Other hormones, including glucocorticoids and androgens, were not significantly different between successful and unsuccessful individuals. Observations of specific behaviors such as unison calling, marching, and the number of copulation attempts, along with overall time spent performing reproductive behaviors, were significantly higher in successful pairs. Our findings indicate that overall reproductive performance of whooping crane pairs is linked to female gonadal hormone excretion and reproductive behaviors, but not to altered adrenal hormone production.

  8. O conceito de prevenção no discurso da Organização Pan Americana da Saude

    Marcus Vinicius Campos

    2002-01-01

    O principal objetivo desta pesquisa é situar a construção e as transformações do conceito de prevenção no discurso da Organização Pan-Americana da Saúde (OPAS). Para isto, será adotada uma perspectiva histórica, analisando os últimos 50 anos do século passado (1950-2000), por meio da metodologia arqueológica de Michel Foucault. O levantamento da produção científica da OPAS baseia-se na análise documental dos periódicos: Boletín de la Oficina Sanitária Panamericana, Educación Médica y Salud e ...

  9. O conceito de prevenção no discurso da Organização Pan Americana da Saude

    Marcus Vinicius Campos

    2002-01-01

    Resumo: O principal objetivo desta pesquisa é situar a construção e as transformações do conceito de prevenção no discurso da Organização Pan-Americana da Saúde (OPAS). Para isto, será adotada uma perspectiva histórica, analisando os últimos 50 anos do século passado (1950-2000), por meio da metodologia arqueológica de Michel Foucault. O levantamento da produção científica da OPAS baseia-se na análise documental dos periódicos: Boletín de la Oficina Sanitária Panamericana, Educación Médica y...

  10. Meiotic pairing of B chromosomes, multiple sexual system, and Robertsonian fusion in the red brocket deer Mazama americana (Mammalia, Cervidae).

    Aquino, C I; Abril, V V; Duarte, J M B

    2013-09-13

    Deer species of the genus Mazama show significant inter- and intraspecific chromosomal variation due to the occurrence of rearrangements and B chromosomes. Given that carriers of aneuploidies and structural rearrangements often show anomalous chromosome pairings, we here performed a synaptonemal complex analysis to study chromosome pairing behavior in a red brocket deer (Mazama americana) individual that is heterozygous for a Robertsonian translocation, is a B chromosome carrier, and has a multiple sex chromosome system (XY₁Y₂). The synaptonemal complex in spermatocytes showed normal chromosome pairings for all chromosomes, including the autosomal and sex trivalents. The electromicrographs showed homology among B chromosomes since they formed bivalents, but they also appeared as univalents, indicating their anomalous behavior and non-Mendelian segregation. Thus, synaptonemal complex analysis is a useful tool to evaluate the role of B chromosomes and rearrangements during meiosis on the intraspecific chromosomal variation that is observed in the majority of Mazama species.

  11. Catechol conjugation with hemolymph proteins and their incorporation into the cuticle of the American cockroach, Periplaneta americana

    Bailey, W.D.; Kimbrough, T.D.; Mills, R.R. [Department of Biology, Virginia Commonwealth University, Richmond, VA 23284-2012 (United States)

    1999-01-01

    Newly ecdysed American cockroaches, Periplaneta americana (six to last instar)were injected with radioactive dopamine. In addition, the reinjection of radiolabeled protein of any size resulted in the incorporation of the label into the newly sclerotized cuticle. Hemolymph proteins were synthesized in vivo using [{sup 14}C]leucine and subsequently double labeled in vivo with [{sup 3}H]dopamine. After sclerotization (7 h post-ecdysis) the cuticle was extirpated, hydrolyzed and counted. An identical ratio of {sup 14}C to {sup 3}H was found in cuticle extracts as in the double-labeled hemolymph proteins, suggesting that the phenol-bound protein was incorporated in the cuticle unchanged. It appears that the catechol bound to the proteins exists as a {beta}-glucoside. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  12. Pauciconfibula subsolana n. sp. (Monogenea: Microcotylidae) from Morone americana (Perciformes: Percichthyidae) collected in fresh water in New Brunswick, Canada.

    Chisholm, L A; Beverley-Burton, M; McAlpine, D F

    1991-12-01

    Pauciconfibula subsolana n. sp. (Monogenea: Microcotylidae) is proposed for parasites found on the inner surface of the operculum of Morone americana collected in the freshwater reaches of the Saint John River, near Mactaquac, New Brunswick. Pauciconfibula subsolana is differentiated from other species in the genus by the 2 posterolateral clamp sclerites, each of which is composed of 2 distinct sections, and by the absence of appendages on the eggs. An amended generic diagnosis is provided for Pauciconfibula. Pseudoaspinatrium Mamaev, 1986, is considered a junior synonym of Pauciconfibula Dillon and Hargis, 1965, and the species previously assigned to Pseudoaspinatrium are transferred to Pauciconfibula as Pauciconfibula euzeti (Ktari, 1971) n. comb., Pauciconfibula gallieni (Euzet and Ktari, 1971) n. comb., and Pauciconfibula pogoniae (MacCallum, 1913) n. comb.

  13. Identification of avocado (Persea americana) pulp proteins by nano-LC-MS/MS via combinatorial peptide ligand libraries.

    Esteve, Clara; D'Amato, Alfonsina; Marina, María Luisa; García, María Concepción; Righetti, Pier Giorgio

    2012-09-01

    Avocado (Persea americana) proteins have been scarcely studied despite their importance, especially in food related allergies. The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries at pH 7.4 and under conditions mimicking reverse-phase capture at pH 2.2. The total number of unique gene products identified amounts to 1012 proteins, of which 174 are in common with the control, untreated sample, 190 are present only in the control and 648 represent the new species detected via combinatorial peptide ligand libraries of all combined eluates and likely represent low-abundance proteins. Among the 1012 proteins, it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein, a glucanase, and an isoflavone reductase like protein.

  14. Endoparasites of American marten (Martes americana: Review of the literature and parasite survey of reintroduced American marten in Michigan

    Maria C. Spriggs

    2016-12-01

    Full Text Available The American marten (Martes americana was reintroduced to both the Upper (UP and northern Lower Peninsula (NLP of Michigan during the 20th century. This is the first report of endoparasites of American marten from the NLP. Faeces from live-trapped American marten were examined for the presence of parasitic ova, and blood samples were obtained for haematocrit evaluation. The most prevalent parasites were Capillaria and Alaria species. Helminth parasites reported in American marten for the first time include Eucoleus boehmi, hookworm, and Hymenolepis and Strongyloides species. This is the first report of shedding of Sarcocystis species sporocysts in an American marten and identification of 2 coccidian parasites, Cystoisospora and Eimeria species. The pathologic and zoonotic potential of each parasite species is discussed, and previous reports of endoparasites of the American marten in North America are reviewed.

  15. Progreso Temporal del Daño por Trips (Insecta:Thysanoptera) en Aguacate (Persea americana Mill.)

    Graciela D. Ávila-Quezada; Daniel Téliz-Ortiz; Humberto Vaquera-Huerta; Héctor González-Hernández; Roberto Johansen-Naime

    2005-01-01

    Se estudió el progreso temporal del daño por trips (Insecta: Thysanoptera) en frutos de aguacate (Persea americana Mill.) cv. Hass en tres huertos en Michoacán, México. La incidencia final del daño fue alta, hasta 61%, principalmente en los huertos de las zonas altas como Zirosto y Tancítaro (2100 de altura), en los que también el Área Bajo la Curva de Progreso (ABCPD) del daño fue alta. El daño se manifestó en marzo y abril, con tasas de infección aparente de 0.05 a 0.15, descritas por el mo...

  16. Leaf anatomy and histochemistry of three species of Ficus sect. Americanae supported by light and electron microscopy.

    Araújo, Nathalia Diniz; Coelho, Victor Peçanha M; Ventrella, Marília Contin; Agra, Maria de Fátima

    2014-02-01

    In this work the leaf anatomy of three species of Ficus section Americanae (Miq.) Miq. from Brazil, whose leaves and latex are used in folk medicine is reported. The work was carried out using light and scanning electron microscopy in order to characterize these species and to evaluate their taxonomic significance, and also contribute to the quality control of their ethnodrugs. The three species (Ficus cyclophylla, Ficus elliotiana, and Ficus caatingae) showed hypostomatic leaves, anomocytic stomata, straight epidermal cell outlines, and a dorsiventral mesophyll. Some micro-morphological characters such as density and distribution of epicuticular waxes, glandular trichomes, the length and width of stomata, as well as the palisade of mesophyll and petiole outlines proved to be the most useful and distinctive characters for the separation of species. These may contribute as additional support for the taxonomy of the section and for the quality control of their ethnodrugs.

  17. A new coccidian, Isospora rheae sp. nov. (Apicomplexa, Eimeriidae, from Rhea americana (Aves, Rheidae from South America

    Samira S.M. Gallo

    2014-12-01

    Full Text Available A new species of coccidian (Protozoa: Apicomplexa: Eimeriidae obtained from rheas, Rhea americana, is reported in Brazil. Oocysts of Isospora rheae sp. nov. are spherical to subspheroidal, measuring 22.6 × 21.0 µm, and have a double and smooth wall that is approximately 1.7 µm thick. The micropyle, oocyst residuum and polar granule are absent. Sporocysts are slightly ovoid, measuring 13.9 × 9.6 µm. The Stieda body is flattened, the substieda body is pointed, irregular and wavy and the sporocyst residuum is composed of scattered granules of varying sizes. Sporozoites have an oblong refractile body and one nucleus. This is the first description of an isosporid coccidian infecting birds of the family Rheidae.

  18. Female gonadal hormones and reproductive behaviors as key determinants of successful reproductive output of breeding whooping cranes (Grus americana).

    Brown, Megan E; Converse, Sarah J; Chandler, Jane N; Shafer, Charles; Brown, Janine L; Keefer, Carol L; Songsasen, Nucharin

    2016-05-01

    Reproductive success of endangered whooping cranes (Grus americana) maintained ex situ is poor. As part of an effort to identify potential causes of poor reproductive success in a captive colony, we used non-invasive endocrine monitoring to assess gonadal and adrenal steroids of bird pairs with various reproductive outcomes and evaluated the relationships of hormones and behaviors to reproductive performance. Overall, reproductively successful (i.e., egg laying) females had significantly higher mean estrogen levels but lower mean progestogen concentrations than did unsuccessful females. Other hormones, including glucocorticoids and androgens, were not significantly different between successful and unsuccessful individuals. Observations of specific behaviors such as unison calling, marching, and the number of copulation attempts, along with overall time spent performing reproductive behaviors, were significantly higher in successful pairs. Our findings indicate that overall reproductive performance of whooping crane pairs is linked to female gonadal hormone excretion and reproductive behaviors, but not to altered adrenal hormone production.

  19. Estudo da Leishmaniose tegumentar americana no Estado do Mato Grosso, no período de 1994 a 1999

    Emilia Emiko Hayashi

    2004-01-01

    A leshmaniose tegumentar americana (LTA) é uma doença de ampla distribuição no continente, constituindo importante problema de saúde pública na América Latina. No Brasil, são notificados cerca de 35 mil casos novos a cada ano, com maior distribuição nas regiões Centro-Oeste, Norte e Nordeste. A doença têm uma forte associação com desmatamentos e o estabelecimento de contingentes populacionais em áreas recentemente desbravadas. A região Centro-Oeste do País, notadamente o estado do Mato Grosso...

  20. Endoparasites of American marten (Martes americana): Review of the literature and parasite survey of reintroduced American marten in Michigan.

    Spriggs, Maria C; Kaloustian, Lisa L; Gerhold, Richard W

    2016-12-01

    The American marten (Martes americana) was reintroduced to both the Upper (UP) and northern Lower Peninsula (NLP) of Michigan during the 20th century. This is the first report of endoparasites of American marten from the NLP. Faeces from live-trapped American marten were examined for the presence of parasitic ova, and blood samples were obtained for haematocrit evaluation. The most prevalent parasites were Capillaria and Alaria species. Helminth parasites reported in American marten for the first time include Eucoleus boehmi, hookworm, and Hymenolepis and Strongyloides species. This is the first report of shedding of Sarcocystis species sporocysts in an American marten and identification of 2 coccidian parasites, Cystoisospora and Eimeria species. The pathologic and zoonotic potential of each parasite species is discussed, and previous reports of endoparasites of the American marten in North America are reviewed.

  1. La vita americana del formaggio: la negoziazione dei valori di un prodotto artigianale in una società industriale

    Heather Paxson

    2015-10-01

    Full Text Available Dove si colloca l’artigianato in un sistema alimentare completamente industrializzato come quello americano? Nel rispondere a questo interrogativo, l’articolo esamina contenuti e significati del formaggio artigianale americano. Partendo dalla mia lunga ricerca etnografica sui casari americani, espongo l’idea che la produzione casearia artigianale americana possa efficacemente intendersi come un “prodotto non finito”, dal valore commerciale e sociale indeterminato e suscettibile di dibattito. Se da un lato i produttori artigiani americani ricavano una soddisfazione personale dal proprio lavoro, dall’altro temono che a lungo andare non trovino ritorno economico. Oltre a cercare di migliorare la pratica produttiva, i casari cercano di comunicare ai consumatori il valore del lavoro nel realizzare un prodotto di “qualità” per il quale vale la pena pagare un prezzo elevato rispetto a quello corrisposto per un prodotto da supermercato. Il valore del formaggio artigianale americano rimane oggetto di negoziazione.

  2. Tracking a genetic signal of extinction-recolonization events in a neotropical tree species: Vouacapoua americana Aublet in French Guiana.

    Dutech, Cyril; Maggia, Laurent; Tardy, Christophe; Joly, Hélène I; Jarne, Philippe

    2003-12-01

    Drier periods from the late Pleistocene and early Holocene have been hypothesized to have caused the disappearance of various rainforest species over large geographical areas in South America and restricted the extant populations to mesic sites. Subsequent improvement in climatic conditions has been associated with recolonization. Changes in population size associated with these extinction-recolonization events should have affected genetic diversity within species. However, these historical hypotheses and their genetic consequences have rarely been tested in South America. Here, we examine the diversity of the chloroplast and nuclear genomes in a Neotropical rainforest tree species, Vouacapoua americana (Leguminosae, Caesalpinioideae) in French Guiana. The chloroplast diversity was analyzed using a polymerase chain reaction-restriction fragment length polymorphism method (six pairs of primers) in 29 populations distributed over most of French Guiana, and a subset of 17 populations was also analyzed at nine polymorphic microsatellite loci. To determine whether this species has experienced extinction-recolonization, we sampled populations in areas supposedly not or only slightly affected by climatic changes, where the populations would not have experienced frequent extinction, and in areas that appear to have been recently recolonized. In the putatively recolonized areas, we found patches of several thousands of hectares homogeneous for chloroplast variation that can be interpreted as the effect of recolonization processes from several geographical origins. In addition, we observed that, for both chloroplast and nuclear genomes, the populations in newly recolonized areas exhibited a significantly smaller allelic richness than others. Controlling for geographic distance, we also detected a significant correlation between chloroplast and nuclear population differentiation. This result indicates a cytonuclear disequilibrium that can be interpreted as a historical signal

  3. Persea americana Glycolic Extract: In Vitro Study of Antimicrobial Activity against Candida albicans Biofilm and Cytotoxicity Evaluation

    Jesus, D.; Oliveira, J. R.; Oliveira, F. E.; Higa, K. C.; Junqueira, J. C.; Jorge, A. O. C.; Back-Brito, G. N.; Oliveira, L. D.

    2015-01-01

    This study evaluated the antifungal activity of Persea americana extract on Candida albicans biofilm and its cytotoxicity in macrophage culture (RAW 264.7). To determine the minimum inhibitory concentration (MIC), microdilution in broth (CLSI M27-S4 protocol) was performed. Thereafter, the concentrations of 12.5, 25, 50, 100, and 200 mg/mL (n = 10) with 5 min exposure were analyzed on mature biofilm in microplate wells for 48 h. Saline was used as control (n = 10). After treatment, biofilm cells were scraped off and dilutions were plated on Sabouraud dextrose agar. After incubation (37°C/48 h), the values of colony forming units per milliliter (CFU/mL) were converted to log10 and analyzed (ANOVA and Tukey test, 5%). The cytotoxicity of the P. americana extract was evaluated on macrophages by MTT assay. The MIC of the extract was 6.25 mg/mL and with 12.5 mg/mL there was elimination of 100% of planktonic cultures. Regarding the biofilms, a significant reduction (P < 0.001) of the biofilm at concentrations of 50 (0.580 ± 0.209 log10), 100 (0.998 ± 0.508 log10), and 200 mg/mL (1.093 ± 0.462 log10) was observed. The concentrations of 200 and 100 mg/mL were cytotoxic for macrophages, while the concentrations of 50, 25, and 12.5 mg/mL showed viability higher than 55%. PMID:26605376

  4. CARACTERIZAÇÃO QUÍMICA E FÍSICA DO JENIPAPO (Genipa americana L. ARMAZENADO

    A. P. da SILVA

    1998-01-01

    Full Text Available As características do jenipapo (Genipa americana L. produzido na região de Botucatu-SP, bem como sua conservação pós-colheita foram estudados quando armazenado sob temperatura ambiente e sob refrigeração (10ºC. Os frutos foram avaliados a cada 7 dias quanto à perda de peso, diâmetro, vitamina C, sólidos solúveis totais, acidez total titulável, pH e firmeza. Ao final do período de armazenamento (28 dias de conservação, os resultados mostraram que a temperatura de 10ºC foi adequada para a conservação do jenipapo e, que as características químicas e físicas dos frutos produzidos na região de Botucatu, são comparativamente semelhantes aos produzidos em outras regiões produtoras, com exceção do teor de sólidos solúveis totais que estão abaixo dos relatados.To study the chemical and physical characteristics of jenipap (Genipa americana L. produced in the Botucatu-SP region, and its post-harvest conservation fruits, were studied when stored at ambient temperature and under refrigeration. The fruits were evaluated every 7 days for weight loss, diameter, vitamin C, total soluble solids, titratable acidity, pH and texture (flesh firmness. At the end of the 4th week of storage the results showed that the temperature of 10ºC was adequate for the conservation of jenipap, and that the chemical and physical characteristcs of the fruits produced in the Botucatu-SP region are similar to those produced in the other regions, except for total soluble solids, which was lower.

  5. Persea americana Glycolic Extract: In Vitro Study of Antimicrobial Activity against Candida albicans Biofilm and Cytotoxicity Evaluation

    D. Jesus

    2015-01-01

    Full Text Available This study evaluated the antifungal activity of Persea americana extract on Candida albicans biofilm and its cytotoxicity in macrophage culture (RAW 264.7. To determine the minimum inhibitory concentration (MIC, microdilution in broth (CLSI M27-S4 protocol was performed. Thereafter, the concentrations of 12.5, 25, 50, 100, and 200 mg/mL (n=10 with 5 min exposure were analyzed on mature biofilm in microplate wells for 48 h. Saline was used as control (n=10. After treatment, biofilm cells were scraped off and dilutions were plated on Sabouraud dextrose agar. After incubation (37°C/48 h, the values of colony forming units per milliliter (CFU/mL were converted to log10 and analyzed (ANOVA and Tukey test, 5%. The cytotoxicity of the P. americana extract was evaluated on macrophages by MTT assay. The MIC of the extract was 6.25 mg/mL and with 12.5 mg/mL there was elimination of 100% of planktonic cultures. Regarding the biofilms, a significant reduction (P<0.001 of the biofilm at concentrations of 50 (0.580±0.209 log10, 100 (0.998±0.508 log10, and 200 mg/mL (1.093±0.462 log10 was observed. The concentrations of 200 and 100 mg/mL were cytotoxic for macrophages, while the concentrations of 50, 25, and 12.5 mg/mL showed viability higher than 55%.

  6. Persea americana Glycolic Extract: In Vitro Study of Antimicrobial Activity against Candida albicans Biofilm and Cytotoxicity Evaluation.

    Jesus, D; Oliveira, J R; Oliveira, F E; Higa, K C; Junqueira, J C; Jorge, A O C; Back-Brito, G N; Oliveira, L D

    2015-01-01

    This study evaluated the antifungal activity of Persea americana extract on Candida albicans biofilm and its cytotoxicity in macrophage culture (RAW 264.7). To determine the minimum inhibitory concentration (MIC), microdilution in broth (CLSI M27-S4 protocol) was performed. Thereafter, the concentrations of 12.5, 25, 50, 100, and 200 mg/mL (n = 10) with 5 min exposure were analyzed on mature biofilm in microplate wells for 48 h. Saline was used as control (n = 10). After treatment, biofilm cells were scraped off and dilutions were plated on Sabouraud dextrose agar. After incubation (37°C/48 h), the values of colony forming units per milliliter (CFU/mL) were converted to log10 and analyzed (ANOVA and Tukey test, 5%). The cytotoxicity of the P. americana extract was evaluated on macrophages by MTT assay. The MIC of the extract was 6.25 mg/mL and with 12.5 mg/mL there was elimination of 100% of planktonic cultures. Regarding the biofilms, a significant reduction (P < 0.001) of the biofilm at concentrations of 50 (0.580 ± 0.209 log10), 100 (0.998 ± 0.508 log10), and 200 mg/mL (1.093 ± 0.462 log10) was observed. The concentrations of 200 and 100 mg/mL were cytotoxic for macrophages, while the concentrations of 50, 25, and 12.5 mg/mL showed viability higher than 55%.

  7. In vitro evaluation of genotoxicity of avocado (Persea americana) fruit and leaf extracts in human peripheral lymphocytes.

    Kulkarni, Paresh; Paul, Rajkumar; Ganesh, N

    2010-07-01

    Persea americana is much sought after both for the nutritional value of its fruit and the medicinal values of its various plant parts. A chromosomal aberration assay was undertaken to evaluate the potential genotoxicity of crude extracts from avocado fruits and leaves. Chromosomal aberrations were observed in cultured human peripheral lymphocytes exposed to separately increasing concentrations of 50% methanolic extracts of Persea americana fruit and leaves. The groups exposed to leaf and fruit extracts, respectively, showed a concentration-dependent increase in chromosomal aberrations as compared to that in a control group. The mean percentage total aberrant metaphases at 100 mg/kg, 200 mg/kg, and 300 mg/kg concentrations of leaf extract were found respectively to be 58 ± 7.05, 72 ± 6.41, and 78 ± 5.98, which were significantly higher (p < 0.0001 each) than that in the control group (6 ± 3.39). The mean percentage total aberrant metaphases at 100 mg/kg, 200 mg/kg, and 300 mg/kg concentrations of fruit extract were found to be 18 ± 5.49, 40 ± 10.00, and 52 ± 10.20, respectively, which were significantly higher (p = 0.033, p < 0.0001, and p < 0.0001, respectively) than that for control (6 ± 3.39). Acrocentric associations and premature centromeric separation were the two most common abnormalities observed in both the exposed groups. The group exposed to leaf extracts also showed a significant number of a variety of other structural aberrations, including breaks, fragments, dicentrics, terminal deletion, minutes, and Robertsonian translocations. The group exposed to leaf extract showed higher frequency of all types of aberrations at equal concentrations as compared to the group exposed to fruit extract.

  8. Cockroaches (periplaneta americana and blattella germanica: reservoirs of multi drug resistant (MDR bacteria in Uyo, Akwa Ibom State

    E.P. Udoinyang

    2012-08-01

    Full Text Available Bacteriological examination of external surfaces and internal guts of Periplaneta americana and Blattela germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were determined using disc diffusion methods. Of the 317 bacteria isolated from the external surfaces and internal guts of P. americana, 275 (86.8% were Gram-negative bacilli, while 42 (13.2% were Gram-positive bacteria. Of the 204 bacterial isolates from B. germanica, 175 (85.8% were Gram negative, while 29 (14.2% were Gram positive bacteria. The bacteria isolated were Salmonella spp, Shigella spp, Staphylococcus aureus, coagulase negative Staphylococcus spp, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Citrobacter freundii, Morganella morganii, Proteus vulgaris, Proteus mirabilis, Enterobacter cloacae and Providencia spp. Among the Gram positive bacteria, only 75.8% S. aureus and 76.5% B. cereus were sensitive to Streptomycin and Gentamycin, while their resistance profiles to antibiotics in decreasing order were as follows: Chloramphenicol (41.7%, Amoxicillin (40.3%, Streptomycin (36.1%, Tetracycline (36.0%, Erythromycin (35.5%, Gentamicin (34.0%, Penicillin (34.6%, Cephalothin (27.8%, Sulfamethoxazole (23.4%, Ciprofloxacin (18.4% and Levofloxacin (17.7%. Less than 50% of E. cloacae and Providencia spp were resistant to Streptomycin, while ˂ 40% of P. vulgaris, K. pneumoniae, and P. aeruginosa were resistant to Chloramphenicol. Of the 353 (67.8% multi drug resistant bacteria, 121 (23.2% were resistant to 3 antibiotics, 232 (65.7% were resistant to 4-10 antibiotics. The antibiotic resistant Salmonella spp. and P. mirabilis had Multiple Antibiotic Resistance (MAR indexes ranging from 0.27 to 0.82. These findings suggest cockroaches in Uyo as potential vectors of medically important multiple drug-resistant bacteria.

  9. Effect of Eleutherine americana Merr. extract on enzymatic activity and enterotoxin production of Staphylococcus aureus in broth and cooked pork.

    Ifesan, Beatrice O T; Voravuthikunchai, Supayang P

    2009-01-01

    Crude ethanolic extract from the bulb of Eleutherine americana was investigated for its inhibitory activities against lipase and protease enzymes and enterotoxin production by Staphylococcus aureus. Eleven isolates that demonstrated high enzyme activity with three reference strains were selected to study the effect of extract on enzyme production. Exposure of the isolates to subminimal inhibitory concentrations, (1/2) minimum inhibitory concentration (MIC) (125 microg/mL), and (1/4)MIC (62.5 microg/mL) of the crude extract resulted in both partial and total inhibition of lipase and protease enzymes. About 15% of the 106 isolates were positive for enterotoxin production with staphylococcal enterotoxin A (11.3%), enterotoxin B (3.7%), and enterotoxin C (10.3%), and no enterotoxin D was produced. The production of staphylococcal enterotoxins A-D in the presence or absence of the crude extract was carried out. In the broth system, the extract reduced enterotoxin production at subminimal inhibitory concentrations compared with the control. At MIC, total enterotoxin inhibition was observed for enterotoxin C production, whereas synthesis of enterotoxins A, B, and D was totally eliminated at 2MIC. The food system study revealed that the extract could delay production of enterotoxins A, B, and C compared with the control. The extract at 2 mg/mL delayed production of toxins A and C for 8 and 4 h, while toxin B was not detected in the pork at 48 h. The ability of E. americana extract to inhibit lipase and protease enzymes and to delay enterotoxin production in food could present it as a novel food additive to combat the growth of S. aureus in food.

  10. Infecções experimentaes na Leishmaniose visceral americana Experimental infections in american visceral leishmaniasis

    Aristides Marques da Cunha

    1938-01-01

    Full Text Available Passando em revista as experiencias de infecção que effectuamos e que acabamos de relatar, vemos que, as culturas isoladas de casos de Leishmaniose visceral americana quer do homem quer do cão, são capazes de infectar hamsters, rhesus e cães de maneira identica ao que acontece com as outras formas de Kala-Azar. Notamos ainda que a evolução da doença e as lesões observadas nos animaes assim infectados, se assemelham ao que tem sido observado nessa enfermidade aproximando-a de maneira singular do Kala-Azar mediterraneo. É sobretudo no cão que a semelhança se torna mais patente. A infecção da pelle e as lesões por ella provocadas que observamos, não differem em nada das que tem sido descriptas na infecção natural do cão e já assignaladas tambem na infecção experimental desse animal, embora de maneira menos completa do que fazemos agora aqui. As lesões oculares com formação de placas de keratite, a infeccção massiça do intestino nos casos fataes de infecção e até mesmo as lesões do figado descriptas por Adler como peculiares á infecção experimental, (infiltração em torno da veia central do lobulo constituem outros tantos caracteres que mostram a completa analogia entre as infecções do cão que obtivemos e as já observadas no Kala-Azar mediterraneo. Além disso, a presença de Leishmanias na pelle, as vezes em grande numero e a constancia dessa localisação parasitaria, vem mostrar que o cão apresenta as condições necessarias para desempenhar o papel de depositario de virus tal como acontece no Kala-Azar mediterraneo, tanto mais que é elle encontrado naturalmente infectado, nas regiões em que grassa a doença. Mas não é só a infecção do cão que mostra essa semelhança; nos outros animaes tambem se verifica o mesmo facto e até pequenos signaes, taes como a tumefacção das patas assignalada nos hamsters infectados com Leishmania infantum, foram tambem observados aqui. Por outro lado, a reacção de

  11. Estudio eco-epidemiológico de la tripanosomiasis americana en el municipio de Amalfi (Antioquia

    J. Arboleda

    2000-02-01

    Full Text Available

    El departamento de Antioquia no ha sido considerado endémico para la enfermedad de Chagas, sin embargo en el nordeste antioqueño, probablemente ocasionado por la tala y quema excesivas y la creciente deforestación para establecer medios de producción agrícola y ganadera, en las áreas naturales, se ha observado la aparición de insectos triatominos al interior de viviendas rurales, alimentándose de sus habitantes.

    Esta situación motivó la realización de un estudio ecoepidemiológico de Tripanosomiasis americana en nueve veredas del municipio de Amalfi con el propósito de identificar las especies de triatominos presentes en la zona, el grado de domiciliación, el índice de infección natural por Trypanosoma cruzi, los porcentajes de infección de animales domésticos (caninos y de mamíferos silvestres, la determinación de la seroprevalencia humana y finalmente la identificación de las acciones del hombre que están llevando a los triatominos a las viviendas para refugio y alimentación.

    En las veredas estudiadas se capturaron 196 triatominos, consideradas especies silvestres: Panstrongylus geniculatus (49%, P. rufotuberculatus (47% y Triatoma dispar (3%. El índice de infestación domiciliaria fue 14.53 % y las veredas con mayor infestación fueron Montebello (32.73 % y La Gardenia (27.5 %.

    Igualmente teniendo en cuenta el total de insectos infectados naturalmente por T. cruzi, fueron estas dos veredas las que obtuvieron el mayor porcentaje de infección 43.75 % y 25.0 % respectivamente.

    En viviendas de la vereda La Gardenia se hallaron huevos y ninfas de P

  12. Photosynthetic Characteristics of Transgenic Bt (Cry1Ab/Ac) Rice Under Ozone Free Air Controlled Enrichment Conditions%开放式臭氧浓度升高条件下转Bt(Cry1Ab/Ac)基因水稻的光合特性

    李春华; 朱建国; 刘标; 曾青; 罗克菊; 唐昊冶; 刘钢; 张国安

    2012-01-01

    [目的]明确臭氧(O3)浓度升高对转Bt基因水稻(Oryza Sativa L.)光合特性的影响.[方法]利用中国农田开放式O3浓度升高(O3-FACE)研究平台,以Bt汕优63 (Bt-SY63)及汕优63 (SY63)为试验材料,采用盆栽种植,分别于处理26 d、47d和75d对其光合特性相关生理指标进行测定分析.[结果]随着O3处理时间的延长,Bt-SY63和SY63剑叶净光合速率(Pn)呈现下降趋势,与各自对照相比75d时分别下降21.1%和15.1%(P<0.01),气孔导度(Gs)和蒸腾速率(Tr)的变化趋势与Pn基本保持一致;PS Ⅱ最大光化学效率、PSⅡ实际光化学效率、光化学猝灭也表现出不同程度的下降;而两品种非光化学猝灭、吸收光能用于PS Ⅱ天线色素耗散部分的变化则是处理高于对照;叶绿素以及类胡萝卜素含量也呈下降趋势,Bt-SY63可溶性蛋白含量在后期处理高于对照,核酮糖-1,5-二磷酸羧化/加氧酶( Rubisco)的含量未显著减少.[结论]03浓度升高使Bt-SY63的光合特性相关指标都发生了不同程度的改变且变幅大于SY63,说明Bt-SY63对O3的响应较SY63敏感,在O3胁迫下,Bt-SY63各性状指标的波动性较大.%[Objective] The purpose of this study was to identify the effects on photosynthetic characteristics of transgenic Bt rice (Oryza saliva L.) under elevated O3 concentration during the period of May-October of 2010. [Method] Pot experiments were conducted with Bt-Shanyou 63 (hereinafter called Bt-SY63, a transgenic rice with Cry1Ab/Ac) and Shanyou 63 (hereinafter called SY63, a hybrid indica cultivar) at 26 d, 47 d and 75 d of ozone treatment, respectively, the indicators relating to the photosynthetic characteristic were measured under Ozone Free Air Controlled Enrichment Conditions (O3-FACE). [Result] The results showed that the net photosynthetic rate of flag leaves in Bt-SY63 and SY63, compared with the control, took on a significantly decline up to 21.1% and 15.1% on 75 d (P<0.01), respectively, by

  13. Desenvolvimento de equipamento para controle mecânico de plantas aquáticas na UHE de Americana-SP Development of equipment for mechanical control of aquatic plants at the Americana reservoir in Brazil

    L.F.N. Bravin

    2005-06-01

    Full Text Available Os objetivos deste trabalho foram desenvolver e avaliar o rendimento operacional de um equipamento para controle de plantas aquáticas, além de estabelecer procedimentos que permitissem a otimização dessa prática na UHE de Americana-SP. O equipamento constitui-se de uma esteira de margem (3,0 m de largura x 10,0 m de comprimento para captação e condução das plantas até um picador, que as fragmenta antes do descarte, facilitando assim sua decomposição e seu transporte. A análise realizada indicou que a capacidade operacional foi de aproximadamente 7,73 m³ h-1. Considerando-se a menor taxa de crescimento observada no histórico do reservatório, 2,27%, o sistema deveria permitir a remoção de aproximadamente 28 m³ h-1. Dessa forma, esse equipamento pode funcionar como um método auxiliar no controle das plantas aquáticas, não realizando um controle efetivo se empregado de forma isolada.The aim of this work was to develop and evaluate the operational capacity of a device for aquatic plant control, as well to establish procedures to improve plant control at the Americana reservoir in Brazil. Basically, the equipment consists of a shore conveyor (3.0 m wide x 10.0 m long for collecting and carrying the plants to a cutter (that breaks the plants before transportation, facilitating their decomposition and transport. The analysis showed that the operational capacity of the equipment was approximately 7.73 m³ h-1. Considering the lowest growth index observed in the reservoir description, 2.27%, the system should remove approximately 28 m³ h-1. This equipment can act as a support for aquatic plant control, without providing an effective control when used alone.

  14. La pena de muerte en la Convención Americana sobre Derechos Humanos y en la jurisprudencia de la Corte Interamericana

    Sergio García Ramírez

    2005-01-01

    En el presente artículo, el autor, a través del estudio de la Convención Americana sobre Derechos Humanos y la jurisprudencia de la Corte Interamericana y otros instrumentos internacionales sobre derechos humanos, discute los criterios y decisiones más importantes adoptadas por la Corte Interamericana de Derechos Humanos sobre la pena de muerte. El estudio de los diversos convenios, convenciones, tratados y jurisprudencia internacional, permite conocer el alcance y aplicabilidad de nuevas opi...

  15. Effect of frozen storage duration and cooking on physical and oxidative changes in M.Gastrocnemius pars interna and M.Iliofibularis of rhea americana

    Schmidt Filgueras, Renata; Gatellier, Philippe; ZAMBIAZI,Rui C.; Sante-Lhoutellier, Veronique

    2010-01-01

    This study was conducted to evaluate the effect of frozen storage time (30, 60, 90 or 180 days) and cooking (100 °C, 30 min) on the physical characteristics and oxidative stability of M. Gastrocnemius pars interna (GN) and M. Iliofiburalis (IF) of rhea americana. Physical parameters measured included thawing and cooking loss, colour parameters (L*a*b*), while oxidation was assessed by determining the TBA-RS, carbonyl and aromatic amino acid content. Prolonged frozen storage of rhea meat decre...

  16. Elementos del bloque constitucional del acceso a la jurisdicción y debido proceso proveniente de la convención americana de derechos humanos

    Humberto Nogueira Alcalá

    2004-01-01

    Full Text Available El presente artículo analiza el derecho de acceso a la jurisdicción y el debido proceso en sus dimensiones formales y sustanciales o materiales, a partir de las normas vigentes de la Convención Americana de Derechos Humanos y del bloque constitucional de derechos, poniendo acento en la doctrina y jurisprudencia emanada de la Corte Interamericana de Derechos Humanos

  17. [Genitalia of three species of Heilipus Germar (Coleoptera: Curculionidae) that damage avocado fruits (Persea americana Mill.) in Mexico and Costa Rica].

    Castañeda-Vildózola, Alvaro; Valdez-Carrasco, Jorge; Equihua-Martínez, Armando; González-Hernández, Héctor; Romero-Nápoles, Jesús; Solís-Aguilar, Juan F; Ramírez-Alarcón, Samuel

    2007-01-01

    The male and female genitaliae of three species of the genus Heilipus Germar (H. lauri Boheman, H. pittieri Barber and H. trifasciatus Fabricius) that damage avocado fruits (Persea americana Mill.) in Mexico and Costa Rica are described and illustrated. The aedeagus, spiculum gastrale, styli of 8th sternite are different in each one of the three species studied and can be used for specific identification.

  18. Alguns aspectos da anatomía-ecológica de Persea americana Mili (abacateiro e interpretações fisio-ecológicas

    Moacyr Eurípedes Medri

    1985-11-01

    Full Text Available In the present work are presented anatomical differences in leaves situaded in different strata on the same tree ofPersea Americana. Analysis of linear regression and tests of coeficience of correlation demonstrate that in respect tb the relationships of the anatomical parameters studied, there is much interdependence. The histological point of view compares such parameters as number and size of stomata, volume of mesophyll, area of functional xylem, phloem, supportive tissue and epidermic tissue, and on the other han different microclimatic positions, domonstrates that Persea Americana is an extremely plastic species with much adaptability; as plastic as Hevea brasiliensis Muelli. Arg and much more plastic than Bertholletia excelsa Humb. & Benpl.No presente trabalho são apresentadas as diferenças anatômicas em folhas situadas em diferentes estratos de uma mesma árvore de Persea americana MUI, mostrando através de análise de regressão linear, coeficiente de correlação conclusivos a, respeito das relações diretas e significativas no tocante aos parâmetros anatômicos estudados, evidenciando interdependências dos mesmos. Do ponto de vista histológico, onde se tomou como parâmetro o número e tamanho de estômatos e volume do mesófilo, área xilemática útil, floema, tecido mecânico e epidérmico, e de outro lado, condições microclimáticas diferentes, P. americana evidencia ser uma espécie bastante plástica, havendo uma relação direta se correlacionada ao seu amplo comportamento adaptativo, sendo tão plástica quanto Hevea brasiliensis Muell. Arg. (Medri, 1977 e muito mais que Bertholletia excelsa Humb. & Benpl. (Medri, 1978.

  19. Elementos del bloque constitucional del acceso a la jurisdicción y debido proceso proveniente de la convención americana de derechos humanos

    2004-01-01

    El presente artículo analiza el derecho de acceso a la jurisdicción y el debido proceso en sus dimensiones formales y sustanciales o materiales, a partir de las normas vigentes de la Convención Americana de Derechos Humanos y del bloque constitucional de derechos, poniendo acento en la doctrina y jurisprudencia emanada de la Corte Interamericana de Derechos Humanos

  20. La visión del otro. Breve historia de la Revista Española de Antropología Americana

    Sánchez Montañés, Emma

    2002-06-01

    Full Text Available Revista de Antropología Americana is one of the first journals of anthropology in Spain to address the discipline in its modern sense, both academically and professionally. Published by the Department of Americanist Anthropology at Complutense University of Madrid, the journal has since its inception been related to teaching and university research, as shown by the contents of its issues, the list of contributors to them and the extensive network of exchanges with other anthropology journals to which it belongs.

    La Revista de Antropología Americana es una de las pioneras en ocuparse en España de la antropología en un sentido moderno, tanto académica como profesionalmente. Editada desde sus comienzos por el Departamento de Antropología Americana de la Universidad Complutense, durante toda su historia ha mantenido la vinculación con la docencia y la investigación universitaria, no sólo en lo que se refiere a sus colaboradores y sus contenidos, sino también a través de la divulgación conseguida por su extensa red de intercambios con otras publicaciones similares.

  1. The Effect of Bio-Fertilizers on Plant Growth and Growth Rate of Grafted Avocado (Persea americana Mill.

    Lazarus Agus Sukamto

    2014-01-01

    Full Text Available Avocado (Persea americana Mill. is considered the most nutritious of all fruits. Avocado fruit contain high unsaturated fat, protein, and energy. It could be eaten fresh for food, drinks, cooking, and cosmetics. Recently, it has become a significant commodity in international trade. Indonesia is the 2nd avocado producing country, but only little quantity of avocado fruits could be exported. The farmers usually grow avocado plants from the seeds, without proper fertilizers in their backyards or small gardens. The problems could be solved by using grafted plants, proper fertilizers, and growing in a large scale of areas. This research was conducted to find out the effect of two liquid bio-fertilizers namely Mega Rhizo and Beyonic StarTmik on the plant growth and growth rate of grafted avocado plants. Some plant growths and growth rates of grafted avocado were influenced significantly by genotype accession, kind of bio-fertilizer, and weather (temperature.  Plant growth and growth rate of most avocado accessions were not significant differences to bio-fertilizer applications, but some avocado accessions on certain months were significant differently. Growth rate ranks of plant height based on accession were no. 10, 28, 13, 1, 5, 2, and 14 consecutively. Those of canopy width were no. 28, 10, 1, 2, 14, 5, and 13 consecutively. Those of trunk diameters were no. 28, 10, 2, 5, 1, 13, and 14 consecutively. All growth rate ranks based on bio-fertilizer were Mega Rhizo, Beyonic StarTmik, and control consecutively.

  2. Modelling Marten (Martes americana Movement Costs in a Boreal Forest: Effects of Grain Size and Thematic Resolution

    Ophélie Planckaert

    2012-01-01

    Full Text Available We investigated landscape resistance to movements of American marten (Martes americana based on snow-tracking data. We generated movement cost maps of the study area with different grain size, thematic resolution, and habitat-specific resistance to movements. We compared simulated tracks obtained from resistance maps to real tracks plotted along transects that we surveyed in winters 2004 to 2008 at the Montmorency Forest, Quebec, Canada. Simulated tracks were located at the intersection between least-cost paths simulated across the study area and transects. We used nearest-neighbour distances between simulated and real tracks to assess the performance of resistance maps and estimate landscape resistance parameters. Simulations with specified costs to movement for open areas, young forest, and mature forest performed better than simpler resistance scenarios, suggesting that resistance to marten movements differed among those landscape attributes that were considered. Simulations with a map grain size of 100 m performed significantly better than 5, 25, and 300 m, possibly because of gap crossing avoidance. Model performance (compared to null model was maximal when resistance to movement in open habitat was set to 20 times higher than in mature forest, but uncertainty around this estimate was large. This research demonstrates that presence-only (point data can be used to parameterize movements using spatially explicit modelling.

  3. DA LEGITIMIDADE À TIRANIA: TOCQUEVILLE E A ONIPOTÊNCIA DA MAIORIA NA DEMOCRACIA NORTE-AMERICANA

    José Reinaldo Felipe Martins Filho

    2013-06-01

    Full Text Available A proposta desse estudo consiste em discutir um dos mais iminentes problemas que concernem às repúblicas democráticas, qual seja: a tirania da maioria sobre as minorias. Para tal, tomaremos como referência as análises desenvolvidas por Alexis de Tocqueville em A Democracia na América, sobretudo no que se referem à onipotência da maioria na democracia norte-americana. Como um dos maiores perigos dos governos democráticos sempre estará a possibilidade de instauração do despotismo, não sob a força do império de um só, como no exemplo da monarquia, mas através da tirania exercida pela maioria. Trata-se, pois, de discutir os limites do poder popular e da força da maioria na democracia, frente ao risco de aniquilamento da liberdade individual e dos grupos minoritários que, sob a égide da homogeneidade social, são socialmente postos à margem dos direitos.

  4. La educación de las élites "americanas" en la España del siglo XVIII

    Álvaro Chaparro Sainz

    2012-07-01

    Full Text Available La llegada al trono de España de la dinastía borbónica trajo consigo reformas en el sistema administrativo y político. Como consecuencia de ello se produjeron cambios en las vías de reclutamiento de los hombres que debían servir al monarca. Creemos que la aparición de nuevas instituciones educativas, más técnicas y especializadas como los Seminarios de Nobles, hay que entenderla en este contexto. Las élites ubicadas en la península utilizaron estas instituciones para formar a sus descendientes, ¿y las élites americanas?, ¿enviaron también ellas a sus parientes a estas instituciones?, ¿utilizaron las mismas vías de reproducción social que las familias de la élite más próxima a la figura del Rey? Este trabajo busca profundizar en la educación de las élites indianas en España y conocer sus prácticas familiares este marco.

  5. A Política Externa Americana e seus críticos American Foreign Politics and its critics

    Ivani Vassoler Froelich

    2005-12-01

    Full Text Available Este artigo toma como referência analítica a teoria da estabilidade hegemônica como ponto de partida para o exame dos objetivos buscados por uma corrente crítica nos Estados Unidos que propõe um mudança de rumo na condução da atual política externa americana. Os conceitos de poder duro e de poder suave, e as implicações de ambos, são discutidos dentro do contexto das ações internacionais dos Estados Unidos, contrapondo as prioridades da Guerra Fria com os novos desafios da era pós-Guerra Fria, a partir dos atentados de 11 de Setembro e a guerra no Iraque.This article employs the theory of hegemonic stability as the analytical tool and the starting point for the examination of the goals sought by a stream of critics in the United States who are proposing changes in the conduction of the current American foreign policy. The concepts of hard power and soft power, and the implications of both, are discussed in the context of external actions of the United States, with a contrast between the Cold War priorities and the new post-Cold War challenges, in the aftermath of the September 11 attacks and the war in Iraq.

  6. Profiling LC-DAD-ESI-TOF MS method for the determination of phenolic metabolites from avocado (Persea americana).

    Hurtado-Fernández, Elena; Carrasco-Pancorbo, Alegría; Fernández-Gutiérrez, Alberto

    2011-03-23

    A powerful HPLC-DAD-ESI-TOF MS method was established for the efficient identification of the chemical constituents in the methanolic extracts of avocado (Persea americana). Separation and detection conditions were optimized by using a standard mix containing 39 compounds belonging to phenolic acids and different categories of flavonoids, analytes that could be potentially present in the avocado extracts. Optimum LC separation was achieved on a Zorbax Eclipse Plus C18 analytical column (4.6×150 mm, 1.8 μm particle size) by gradient elution with water+acetic acid (0.5%) and acetonitrile as mobile phases, at a flow rate of 1.6 mL/min. The detection was carried out by ultraviolet-visible absorption and ESI-TOF MS. The developed method was applied to the study of 3 different varieties of avocado, and 17 compounds were unequivocally identified with standards. Moreover, around 25 analytes were tentatively identified by taking into account the accuracy and isotopic information provided by TOF MS.

  7. PENGEMBANGAN FORMULASI MASKER EKSTRAK AIR DAUN ALPUKAT (Persea americana Mill SEBAGAI ANTIBAKTERI Staphylococcus aureus UNTUK PENGOBATAN JERAWAT

    Nur Ismiyati

    2014-05-01

    Full Text Available Skin problems are often on the spotlight, one of them is acne. Inflammation of acne can be triggered by the bacteria Propionibacterium acne, Staphylococcus epidermidis and Staphylococcus aureus. One of the herbs that can be used for acne treatment is the leaves of avocado (Persea americana. This study aims to determine the potential antibacterial activity of avocado leaf water extract on the growth of Staphylococcus aureus bacteria that causes acne and mask-making formulation preparation. Antibacterial activity test was carried out by the diffusion method and the observed inhibition zone that occured in a series of concentrations of avocado leaf water extract ( 15%, 17.5% , 35% , 50% , 75%. Antibacterial activity test was also conducted on some avocado leaf water extract mask formula (17.5% , 35% , 50% , 75% was evaluated the physical properties of the mask and irritation test was done. The results showed that the avocado leaf water extract mask formula had antibacterial activity greater than the extract form. The physical condition evaluation and irritation test showed that the best mask formulation was concentration of 35%.

  8. Isolation and chemical identification of lipid derivatives from avocado (Persea americana) pulp with antiplatelet and antithrombotic activities.

    Rodriguez-Sanchez, Dariana Graciela; Flores-García, Mirthala; Silva-Platas, Christian; Rizzo, Sheryl; Torre-Amione, Guillermo; De la Peña-Diaz, Aurora; Hernández-Brenes, Carmen; García-Rivas, Gerardo

    2015-01-01

    Platelets play a pivotal role in physiological hemostasis. However, in coronary arteries damaged by atherosclerosis, enhanced platelet aggregation, with subsequent thrombus formation, is a precipitating factor in acute ischemic events. Avocado pulp (Persea americana) is a good source of bioactive compounds, and its inclusion in the diet as a source of fatty acid has been related to reduced platelet aggregability. Nevertheless, constituents of avocado pulp with antiplatelet activity remain unknown. The present study aims to characterize the chemical nature of avocado constituents with inhibitory effects on platelet aggregation. Centrifugal partition chromatography (CPC) was used as a fractionation and purification tool, guided by an in vitro adenosine diphosphate (ADP), arachidonic acid or collagen-platelet aggregation assay. Antiplatelet activity was initially linked to seven acetogenins that were further purified, and their dose-dependent effects in the presence of various agonists were contrasted. This process led to the identification of Persenone-C (3) as the most potent antiplatelet acetogenin (IC₅₀=3.4 mM) among the evaluated compounds. In vivo evaluations with Persenone A (4) demonstrated potential protective effects against arterial thrombosis (25 mg kg⁻¹ of body weight), as coagulation times increased (2-fold with respect to the vehicle) and thrombus formation was attenuated (71% versus vehicle). From these results, avocado may be referred to as a functional food containing acetogenin compounds that inhibit platelet aggregation with a potential preventive effect on thrombus formation, such as those that occur in ischaemic diseases.

  9. Avocado fruit (Persea americana Mill) exhibits chemo-protective potentiality against cyclophosphamide induced genotoxicity in human lymphocyte culture.

    Paul, Rajkumar; Kulkarni, Paresh; Ganesh, Narayan

    2011-01-01

    Diets rich in fruits and vegetables have been associated with reduced risks for many types of cancers. Avocado (Persea americana Mill.) is a widely consumed fruit containing many cancer preventing nutrients, vitamins and phytochemicals. Studies have shown that phytochemicals extracted from the avocado fruit selectively induce cell cycle arrest, inhibit growth, and induce apoptosis in precancerous and cancer cell lines. Our recent studies indicate that phytochemicals extracted with 50% Methanol from avocado fruits help in proliferation of human lymphocyte cells and decrease chromosomal aberrations induced by cyclophosphamide. Among three concentrations (100 mg, 150 mg and 200 mg per Kg Body Weight), the most effective conc. of extract was 200 mg/Kg Body Wt. It decreased significant level of numerical and structural aberrations (breaks, premature centromeric division etc. up to 88%, p < 0.0001)), and accrocentric associtation within D & G group (up to 78%, p = 0.0008). These studies suggest that phytochemicals from the avocado fruit can be utilized for making active chemoprotective ingredient for lowering the side effect of chemotherapy like cyclophosphamide in cancer therapy.

  10. Impact of Laurel Wilt, Caused by Raffaelea lauricola, on Leaf Gas Exchange and Xylem Sap Flow in Avocado, Persea americana.

    Ploetz, Randy C; Schaffer, Bruce; Vargas, Ana I; Konkol, Joshua L; Salvatierra, Juanpablo; Wideman, Ronney

    2015-04-01

    Laurel wilt, caused by Raffaelea lauricola, is a destructive disease of avocado (Persea americana). The susceptibility of different cultivars and races was examined previously but more information is needed on how this host responds to the disease. In the present study, net CO2 assimilation (A), stomatal conductance of H2O (gs), transpiration (E), water use efficiency (WUE), and xylem sap flow rates were assessed in cultivars that differed in susceptibility. After artificial inoculation with R. lauricola, there was a close relationship between symptom development and reductions in A, gs, E, WUE, and mean daily sap flow in the most susceptible cultivar, 'Russell', and significantly greater disease and lower A, gs, E, WUE, and sap flow rates were usually detected after 15 days compared with the more tolerant 'Brogdon' and 'Marcus Pumpkin'. Significant differences in preinoculation A, gs, E, and WUE were generally not detected among the cultivars but preinoculation sap flow rates were greater in Russell than in Brogdon and Marcus Pumpkin. Preinoculation sap flow rates and symptom severity for individual trees were correlated at the end of an experiment (r=0.46), indicating that a plant's susceptibility to laurel wilt was related to its ability to conduct water. The potential management of this disease with clonal rootstocks that reduce sap flow rates is discussed.

  11. Diurnal time-activity budgets of redheads (Aythya americana) wintering in seagrass beds and coastal ponds in Louisiana and Texas

    Michot, T.C.; Woodin, M.C.; Adair, S.E.; Moser, E.B.

    2006-01-01

    Diurnal time-activity budgets were determined for wintering redheads (Aythya americana) from estuarine seagrass beds in Louisiana (Chandeleur Sound) and Texas (Laguna Madre) and from ponds adjacent to the Laguna Madre. Activities differed (pLaguna Madre or freshwater ponds. Redheads in Louisiana fed more than birds in Texas; this was partially because of weather differences (colder temperatures in Louisiana), but the location effect was still significant even when we adjusted the model for weather effects. Redheads in Louisiana showed increased resting and decreased feeding as winter progressed, but redheads in Texas did not exhibit a seasonal pattern in either resting or feeding. In Louisiana, birds maintained a high level of feeding activity during the early morning throughout the winter, whereas afternoon feeding tapered off in mid- to late-winter. Texas birds showed a shift from morning feeding in early winter to afternoon feeding in late winter. Males and females at both Chandeleur Sound and Laguna Madre showed differences in their activities, but because the absolute difference seldom exceeded 2%, biological significance is questionable. Diurnal time-activity budgets of redheads on the wintering grounds are influenced by water salinities and the use of dietary fresh water, as well as by weather conditions, tides, and perhaps vegetation differences between sites. The opportunity to osmoregulate via dietary freshwater, vs. via nasal salt glands, may have a significant effect on behavioral allocations. ?? Springer 2006.

  12. Cadmium and manganese accumulation in Phytolacca americana L. and the roles of non-protein thiols and organic acids.

    Gao, Lu; Peng, Kejian; Xia, Yan; Wang, Guiping; Niu, Liyuan; Lian, Chunlan; Shen, Zhenguo

    2013-01-01

    Phytolacca americana L. can accumulate large amounts of heavy metals in its aerial tissues, especially cadmium (Cd) and manganese (Mn). It has great potential for use in phytoextraction of metals from multi-metal-contaminated soils. This study was conducted to further investigate the Cd- and Mn-tolerance strategies of this plant. Concentrations of non-protein thiols (NPTs) and phytochelatins (PCs) in leaves and roots increased significantly as the concentration of Cd in solution increased. The molar ratios of PCs:soluble Cd ranged from 1.8 to 3.6 in roots and 8.1 to 31.6 in leaves, suggesting that the cellular response involving PC synthesis was sufficient to complex Cd ions in the cytosol, especially that of leaves. In contrast, excess Mn treatments did not result in a significant increase in NPT or PC concentrations in leaves or roots. Oxalic acid concentrations in leaves of plants exposed to 2 or 20 mM Mn reached 69.4 to 89.3 mg (0.771 to 0.992 mmol) g(-1) dry weight, respectively, which was approximately 3.7- to 8.6-fold higher than the Mn level in the 0.6 M HCl extract. Thus, oxalic acid may play an important role in the detoxification of Mn.

  13. Interplay between a Wnt-dependent organiser and the Notch segmentation clock regulates posterior development in Periplaneta americana

    John E. Chesebro

    2012-12-01

    Sequential addition of segments in the posteriorly growing end of the embryo is a developmental mechanism common to many bilaterians. However, posterior growth and patterning in most animals also entails the establishment of a ‘posterior organiser’ that expresses the Caudal and Wnt proteins and has been proposed to be an ancestral feature of animal development. We have studied the functional relationships between the Wnt-driven organiser and the segmentation mechanisms in a basal insect, the cockroach Periplaneta americana. Here, posteriorly-expressed Wnt1 promotes caudal and Delta expression early in development to generate a growth zone from which segments will later bud off. caudal maintains the undifferentiated growth zone by dampening Delta expression, and hence Notch-mediated segmentation occurs just outside the caudal domain. In turn, Delta expression maintains Wnt1, maintaining this posterior gene network until all segments have formed. This feedback between caudal, Wnt and Notch-signalling in regulating growth and segmentation seems conserved in other arthropods, with some aspects found even in vertebrates. Thus our findings not only support an ancestral Wnt posterior organiser, but also impinge on the proposals for a common origin of segmentation in arthropods, annelids and vertebrates.

  14. Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae).

    Oliveira, M L; Duarte, J M B

    2013-01-16

    We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20'S, 47°17'W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as "fresh" and 36 as "non-fresh". DNA was extracted using the QIAamp(®) DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples. Five microsatellite loci were amplified by PCR; success in amplification varied according to locus size and sample age. Successful amplifications were achieved in 10/16 of the fresh and in 13/36 of the non-fresh samples; a negative correlation (R = -0.82) was found between successful amplification and locus size. Amplification of the amelogenin locus was successful in 22 of the 52 samples. The difficulty of amplifying nuclear loci in DNA samples extracted from feces collected in the field was evident. Some methodological improvements, including collecting fresh samples, selecting primers for shorter loci and quantifying the extracted DNA by real-time PCR, are suggested to increase amplification success in future studies.

  15. Chemical Composition, Functional Properties, and Effect of Inulin from Tunisian Agave americana L. Leaves on Textural Qualities of Pectin Gel

    Mohamed Ali Bouaziz

    2014-01-01

    Full Text Available In this study, the chemical composition and functional properties of Agave americana L. (AA leaves were determined. The Agave leaves powder had a high amount of total dietary fiber (38.40%, total sugars (45.83%, and protein (35.33%, with a relatively low content in ash (5.94% and lipid (2.03%. The Agave leaves were exhibited with potential food application. The Agave inulin showed a significant difference compared with the commercial inulin as for aw (0.275 against 0.282, pH (5.53 against 5.98, ash (2.89% against 1.19%, protein (3.46% against 1.58%, water holding capacity (2.42% against 1.59%, solubility (73 g/L against 113 g/L, and emulsion capacity (14.48% against 21.42%, respectively. The textural properties of Agave inulin-pectin mixed gels were examined using instrumental Texture Profile Analysis (TPA. Firmness of the prepared Agave inulin-pectin mixed gels was lower than the pectin gel (0.3554 N against 5.7238 N, resp.. This reduction of firmness showed a synergetic effect between pectin and inulin. These results suggested a positive interaction between Agave inulin and pectin to decrease the firmness of mixed gels and open a good alternative to obtain value added products from this resource.

  16. Aspectos eco-epidemiológicos da leishmaniose tegumentar americana no Município de Campinas

    Antônio Ângelo Corte

    1996-12-01

    Full Text Available Os autores descrevem um surto de leishmaniose tegumentar americana ocorrido no período de fevereiro de 1993 a setembro de 1994, nos Distritos de Sousas e Joaquim Egídio, Município de Campinas, SP. Ocorreram 25 casos, sete na área rural, nove na área peri-urbana e nove na urbana. Verifica-se uma mobilidade da ocorrência dos casos em direção rural-urbana. Em relação ao sexo, houve predomínio do masculino (60% sobre o feminino (40%, com 44% dos casos concentrados na faixa etária de 11 e trinta anos. Quanto à ocupação, 48% correspondem a estudantes, empregadas domésticas e donas-de-casa. Em 92% dos casos, a doença manifestou-se com lesão única, predominantemente localizadas nos membros inferiores e superiores. O surgimento desses casos na área está provavelmente associado à expansão urbana ocorrida nas décadas de 70-80 e acelerada mais recentemente.

  17. Aspectos eco-epidemiológicos da leishmaniose tegumentar americana no Município de Campinas

    Corte Antônio Ângelo

    1996-01-01

    Full Text Available Os autores descrevem um surto de leishmaniose tegumentar americana ocorrido no período de fevereiro de 1993 a setembro de 1994, nos Distritos de Sousas e Joaquim Egídio, Município de Campinas, SP. Ocorreram 25 casos, sete na área rural, nove na área peri-urbana e nove na urbana. Verifica-se uma mobilidade da ocorrência dos casos em direção rural-urbana. Em relação ao sexo, houve predomínio do masculino (60% sobre o feminino (40%, com 44% dos casos concentrados na faixa etária de 11 e trinta anos. Quanto à ocupação, 48% correspondem a estudantes, empregadas domésticas e donas-de-casa. Em 92% dos casos, a doença manifestou-se com lesão única, predominantemente localizadas nos membros inferiores e superiores. O surgimento desses casos na área está provavelmente associado à expansão urbana ocorrida nas décadas de 70-80 e acelerada mais recentemente.

  18. Behavioural responses of captive-born greater rheas Rhea americana Linnaeus (Rheiformes, Rheidae submitted to antipredator training Respostas comportamentais de emas cativas Rhea americana Linnaeus (Rheiformes, Rheidae submetidas a treinamentoanti-predação

    Cristiano S. de Azevedo

    2006-03-01

    Full Text Available Human activities have been diminishing greater rhea Rhea americana (Linnaeus, 1758 populations throughout their natural distribution. The reintroductions of captive-born greater rheas have been tried but without success; since the individuals reintroduced were killed by predators. Captive-born animals that have been isolated from predators for many generations can lose their predator recognition abilities. To enhance the survival rates of the reintroduced animals, researchers are now using antipredator training techniques. We studied the response of 15 zoo-borne greater rheas to antipredator training. The animals were divided into three groups: two test groups and one control group. We ran 15 antipredator tests and four control testes with each group. Antipredator tests consisted of pairing a taxidermized predator model or a real predator (domestic dog with a simulation of a capture procedure. Control tests consisted of presenting the predator model (jaguar to the birds, after training but not associating it with an aversive event and recording behavioural responses. All tests were video-recorded and analysed a posteriori. Results showed that the trained rheas responded appropriately to the predators, becoming more vigilant and that there was considerable individual differences in response to antipredator training. The results demonstrated that antipredator training is effective and therefore an invaluable tool for reintroduction projects involving greater rheas. Furthermore, the methods employed in this research project should be applicable to other species of flightless birds.As atividades humanas têm diminuído as populações de emas Rhea americana (Linnaeus, 1758 por toda sua área de distribuição. Reintroduções de emas nascidas em cativeiro têm sido tentadas, mas sem sucesso, uma vez que os indivíduos reintroduzidos são mortos por predadores. Animais nascidos em cativeiro que foram isolados de seus predadores por várias gera

  19. Caracterización química foliar de los árboles de aguacate criollo (Persea americana var. drymifolia en los bancos de germoplasma de Michoacán, México Foliar chemical caracterization of the creole avocado trees (Persea americana var. drymifolia in the germplasm banks from Michoacán, Mexico

    Cintia A. Rincón-Hernández

    2011-06-01

    Full Text Available Persea americana var. drymifolia (aguacate criollo mexicano se emplea como portainjerto en huertos de aguacate Hass y es fuente de genes de resistencia a plagas y patógenos para éste y otros cultivares. El aguacate criollo mexicano se está sustituyendo por cultivares comerciales o perdiendo por la destrucción de ecosistemas. Por eso, se estudiaron 250 árboles (52 accesiones de 14 estados de México; 247 de criollo mexicano y 3 híbridos de éste con P.shiedeana, P. americana var. americana y P. americana var. guatemalensis, plantados en los bancos de germoplasma en Michoacán. Se analizó la composición química mediante cromatografía de gases-masas. Hubo alta variabilidad química entre los árboles del banco de germoplasma con los 64 compuestos detectados. El análisis de cúmulos mostró 2 grandes grupos químicos con 100% disimilitud y 22 grupos a 10% de disimilitud. Los híbridos se agruparon de manera diferente a los árboles con características típicas de criollo y difieren en su altitud de origen. Corroboramos que los volátiles foliares sirven como marcadores para diferenciar los criollos típicos de sus híbridos y que mucha variación atribuida a la variedad criolla proviene de hibridaciones en zonas de contacto de esta variedad con otras variedades o especies de Persea.Persea americana var. drymifolia (mexican creole avocado is used as rootstock in Hass avocado orchards and it is a source of genes for resistance to pests and pathogens for this and other cultivars. The Mexican varieties are being replaced by commercial cultivars or lost by the destruction of ecosystems. Therefore, we studied 250 avocado trees of 52 accessions from 14 Mexican states; 247 mexican creole avocado and 3 hybrids of that tree with P. shiedeana, P. americana var. americana or P. americana var. guatemalensis, planted at germplasm banks, Michoacán. Foliar chemical composition was analyzed by GC-MS. High chemical variability among the trees was found

  20. Las plantas americanas en la obra de Charles de l'Écluse: primeras citas en las cartas de Juan de Castañeda

    Ramón-Laca Menéndez de Luarca, Luis

    1999-06-01

    Full Text Available The Flemish botanist Charles de l'Écluse's works contain abundant references to American plants, for example the avocado (Persea americana Mill., the sweet potato [Ipomoea batatas (L. Lam.], the potato (Solanum tuberosum L. and the tobáceo (Nicotiana tabacum L.. Although some of these plants were observed by L'Écluse himself during his expedition to the Iberian Península between 1564 and 1565, the majority of the American plants quoted in his works are related with shipments received from several agents around Europe. In the case of Spain, L'Écluse obtained seeds from Seville thanks to the help of Simón de Tovar and Juan de Castañeda. The latter offers in his letters important innovations: Annona cherimola Mill., Indigofera añil LJI. tinctoria L., Annona muricata L., Mammea americana L.. Annona reticulata L., Dioscorea sp., Aloysia citrodora Palau or Manihot spj M. esculenta Crantz. In most of the cases the mention of these plants represente the first record of cultivation in the Old World. A percentage of this material was used by L'Écluse in his Exoticorum libri decem, published in Antwerp in l605.Las obras del botánico flamenco Charles de l'Écluse contienen infinidad de referencias a plantas americanas, destacando entre ellas el aguacate (Persea americana Mill., las batatas [Ipomoea batatas (L. Lam.], las patatas (Solanum tuberosum L. o el tabaco (Nicotiana tabacum L. . Algunas de ellas fueron observadas por L'Écluse durante la expedición llevada a cabo por la Península Ibérica entre 1564 y 1565. La mayor parte de plantas americanas citadas en sus obras están relacionadas sin embargo con envíos realizados por diversos corresponsales repartidos por Europa. En el caso de España, L'Écluse recibió semillas desde Sevilla gracias a Simón de Tovar y Juan de Castañeda. Este último ofrece en sus cartas importantes novedades, como el "anón" (Annona cherimola Mill., el "añil" (Indigofera añil L./I. tinctoria L., la "guan

  1. Los cariotipos de Cologania grandiflora y Erythrina americana (Leguminosae- Papilionoideae-Phaseoleae de la Reserva Ecológica del Pedregal de San Ángel, México Karyotypes of Cologania grandiflora and Erythrina americana (Leguminosae-Papilionoideae- Phaseoleae of Reserva Ecológica del Pedregal de San Ángel, Mexico

    Fernando Tapia-Pastrana

    2011-09-01

    Full Text Available Se analizaron citogenéticamente células provenientes de meristemos radiculares de 2 leguminosas, Cologania grandiflora y Erythrina americana, que en la actualidad están incluidas en la flora de la Reserva Ecológica del Pedregal de San Ángel, México, D. F., mediante una técnica de extendido en superficie y secado al aire para determinar los números cromosómicos somáticos. Por vez primera se obtuvieron la morfología cromosómica y otras características cuantitativas de los cariotipos en C. grandiflora (2n= 44= 26m +18sm y en E. americana (2n= 42= 36m + 4sm +2st sat, primeras también en ambos géneros. Cologania y Erythrina se reconocen como poliploides estabilizados y el hallazgo de un único par de cromosomas con satélites (dominancia nucleolar en las especies estudiadas aquí, favorece la opinión de un origen alopoliploide para estos taxa.Meristematic root cells from Cologania grandiflora and Erythrina americana from Reserva Ecológica del Pedregal de San Ángel, Distrito Federal, Mexico, were analyzed cytogenenetically using a surface-spreading and air-drying method. The somatic chromosome numbers were determined. Chromosome morphology and others quantitative features of the karyotypes obtained for first time in C. grandiflora (2n= 44= 26m + 18sm and E. americana (2n= 42= 36m + 4sm + 2st sat and also the first in both genera. Cologania and Erythrina are recognized as stabilized polyploids and the finding of just one pair of chromosomes with satellites (nucleolar dominance in the species analyzed here supports the view of allopolyploid origin of these taxa.

  2. Emprego da associação sulfametoxazol + trimetoprim no tratamento da paracoccidioidose (blastomicose Sul-Americana

    P. N. Pedrosa

    1974-06-01

    Full Text Available Acentuando a necessidade da continuação das pesquisas terapêuticas em pacientes com paracoccidioidose (blastomicose sul-americana, os autores apresentam 23 pacientes submetidos a diferentes esquemas terapêuticos, sendo 14 virgens de tratamento e 9 com uso prévio de uma ou mais drogas. A associação sulfametoxazol + trimetoprim ( SMZ + TMP foi empregada em 5 pacientes virgens de tratamento e 9 sulfa-resistentes. Outros sete foram submetidos ao esquema clássico com sulfadoxina. Os pacientes que não responderam aos dois esquemas anteriores, com exceção de dois casos inicialmente graves, receberam anfotericina B. A avaliação clínica, radiológica, micológica e sorológica a longo prazo não demonstrou vantagens no emprego de SMZ + TMP em substituição aos sulfamídicos, nos pacientes virgens de tratamento. Entretanto, a associação SMZ + TMP parece ser uma opção válida nos casos sulfa-resistentes, onde teria primazia, considerando-se a toxicidade e necessidade de controle em regime hospitalar da anfotericina B. Ressaltam ainda a boa tolerância clínica e laboratorial da associação SMZ + TMP em cursos terapêuticos prolongados de até 2 anos, quando empregadas em baixas doses de manutenção.

  3. Supplemental feeding for ecotourism reverses diel activity and alters movement patterns and spatial distribution of the southern stingray, Dasyatis americana.

    Corcoran, Mark J; Wetherbee, Bradley M; Shivji, Mahmood S; Potenski, Matthew D; Chapman, Demian D; Harvey, Guy M

    2013-01-01

    Southern stingrays, Dasyatis americana, have been provided supplemental food in ecotourism operations at Stingray City Sandbar (SCS), Grand Cayman since 1986, with this site becoming one of the world's most famous and heavily visited marine wildlife interaction venues. Given expansion of marine wildlife interactive tourism worldwide, there are questions about the effects of such activities on the focal species and their ecosystems. We used a combination of acoustic telemetry and tag-recapture efforts to test the hypothesis that human-sourced supplemental feeding has altered stingray activity patterns and habitat use at SCS relative to wild animals at control sites. Secondarily, we also qualitatively estimated the population size of stingrays supporting this major ecotourism venue. Tag-recapture data indicated that a population of at least 164 stingrays, over 80% female, utilized the small area at SCS for prolonged periods of time. Examination of comparative movements of mature female stingrays at SCS and control sites revealed strong differences between the two groups: The fed animals demonstrated a notable inversion of diel activity, being constantly active during the day with little movement at night compared to the nocturnally active wild stingrays; The fed stingrays utilized significantly (pstingrays showed a high degree of overlap in their core activity spaces compared to wild stingrays which were largely solitary in the spaces utilized (72% vs. 3% overlap respectively). Supplemental feeding has strikingly altered movement behavior and spatial distribution of the stingrays, and generated an atypically high density of animals at SCS which could have downstream fitness costs for individuals and potentially broader ecosystem effects. These findings should help environmental managers plan mitigating measures for existing operations, and develop precautionary policies regarding proposed feeding sites.

  4. Supplemental feeding for ecotourism reverses diel activity and alters movement patterns and spatial distribution of the southern stingray, Dasyatis americana.

    Mark J Corcoran

    Full Text Available Southern stingrays, Dasyatis americana, have been provided supplemental food in ecotourism operations at Stingray City Sandbar (SCS, Grand Cayman since 1986, with this site becoming one of the world's most famous and heavily visited marine wildlife interaction venues. Given expansion of marine wildlife interactive tourism worldwide, there are questions about the effects of such activities on the focal species and their ecosystems. We used a combination of acoustic telemetry and tag-recapture efforts to test the hypothesis that human-sourced supplemental feeding has altered stingray activity patterns and habitat use at SCS relative to wild animals at control sites. Secondarily, we also qualitatively estimated the population size of stingrays supporting this major ecotourism venue. Tag-recapture data indicated that a population of at least 164 stingrays, over 80% female, utilized the small area at SCS for prolonged periods of time. Examination of comparative movements of mature female stingrays at SCS and control sites revealed strong differences between the two groups: The fed animals demonstrated a notable inversion of diel activity, being constantly active during the day with little movement at night compared to the nocturnally active wild stingrays; The fed stingrays utilized significantly (p<0.05 smaller 24 hour activity spaces compared to wild conspecifics, staying in close proximity to the ecotourism site; Fed stingrays showed a high degree of overlap in their core activity spaces compared to wild stingrays which were largely solitary in the spaces utilized (72% vs. 3% overlap respectively. Supplemental feeding has strikingly altered movement behavior and spatial distribution of the stingrays, and generated an atypically high density of animals at SCS which could have downstream fitness costs for individuals and potentially broader ecosystem effects. These findings should help environmental managers plan mitigating measures for existing

  5. Aprender versus ensinar: Charles Sanders Peirce e a universidade americana do final do século XIX

    Lauro Frederico Barbosa da Silveira

    1982-12-01

    Full Text Available A produção científica e filosófica de Charles Sanders PEIRCE (1839-1914, exigindo como critério para o trabalho intelectual e para a conduta da vida do pensador o absoluto rigor na construção dos conceitos e a estrita verificação experimental, teve por conseqüência desvincular o trabalho científico e filosófico de qualquer função apologética. A afirmação de que todo conhecimento do mundo da experiência e mesmo daquele elaborado pela matemática é intrinsecamente provável e falível se opôs a todo e qualquer dogmatismo e mesmo ao "a priori" de tradição Kantiana. O interesse pela teoria evolucionista e a coerência inabalável da filosofia e das atitudes de PEIRCE, como professor e pesquisador, encontraram profunda resistência no meio universitário e editorial de seu tempo. Num momento de grave crise na Universidade norte-americana, decorrente das transformações econômicas e políticas ocorridas com a guerra da Secessão (1861-1865, o posicionamento de PEIRCE contribuiu muito provavelmente para sua demissão como professor das Universidades de Harvard e de John Hopkins; para dificultar a publicação de seus escritos e para seu total isolamento nos últimos anos de vida.

  6. Reproductive characteristics of captive greater rhea (Rhea americana males reared in the state of São Paulo, Brazil

    PAA Góes

    2010-03-01

    Full Text Available Rheas (Rhea americana belongs to the ratite group. Considering the commercial significance of this birds, some techniques, such as semen collection, were standardized. In this study, 107 male rheas (3 to 4 years of age reared in commercial farms in the state of São Paulo, Brazil, were used. Semen was collected during the breeding and off-breeding seasons of 2001, 2002, and 2003. Bird hierarchical behavior was observed. Birds were restrained performed using a box and a black hood. Semen was collected by digital pressure on the base of the phallus, which size was measured, and the presence or absence of spiral shape was observed. Immediately after collection, semen samples were evaluated for volume, motility, sperm concentration, and morphology. In a limited number of birds, blood samples were collected to measure testosterone levels. Among the 69 birds studied during the breeding season, 44 presented large phalluses, out of which 26 showed spiral shape. The method of semen collection was efficient. The following semen parameter results were obtained: volume (0.68 ±0.14 ml, motility (61.11±11.54%, sperm concentration (3.29±1.33 x10(9 sptz/ml, and number of spermatozoa per ejaculate (2.40±1.38x10(9 sptz/ml. Morphological abnormalities were analyzed and recorded. Testosterone levels were statistically different (p = 0.0161 between the breeding and non-breeding season (53.28±18.41 ng/ml and 5:57±3.81 ng/ml, respectively. Variations in phallus size were also found between the breeding and non-breeding seasons. Larger phalluses and higher testosterone levels were correlated with dominant behavior. The results of the present experiment confirmed that it is possible to collect semen from rheas, allowing the future use of biotechnologies such as artificial insemination.

  7. Tratamento da Leishmaniose tegumentar americana, com lesão em mucosa, por meio do isotionato de pentamidina

    Valdir Sabbaga Amato

    1996-10-01

    Full Text Available Dez pacientes com leishmaniose tegumentar americana, acometidos de lesão em mucosa, foram tratados por meio do isotionato de pentamidina na dose de 4mg/kg, em dias alternados, por via endovenosa. A posologia média correspondeu a 2.140mg. A cicatrização das lesões ocoireu em 9 (90% dos pacientes que completaram o tratamento. Não houve recidiva no período de acompanhamento de 1 a 24 meses (média de 7, 7 meses. Uma paciente interrompeu o tratamento, antes da cicatrização da lesão, por ter desenvolvido diabetes melito. Em 3 (30% pacientes, o exame de sangue mostrou aumento da uréia e da creatinina e leucopenia, corrigido pelo espaçamento da administração do medicamento. O isotionato de pentamidina é eficiente na cicatrização das lesões, mas hã necessidade de melhor avaliação de seu valor na prevenção das recidivas.Ten patients with mucosal lesions caused by American tegumental leishmaniasis were treated with pentamidine isothianate at the dose 4 mg/kg on alternate days by the intravenous route. The mean posology was 2,140mg. Healing of the lesions occurred in 9 (90% of the patients who completed treatment. There was no recurrence during a follow- up time of 1 to 24 months (mean, 7,7 months. One patient discontinued treatment before healing of the lesion because he developed diabetes mellitus. In 3 (30%patients, blood exams showed increased urea and creatinine levels and leucopenia, which were corrected by increasing the interval between administrations of the drug. Pmtamidine isothianate is efficient in bringing about cicatrization of the lesions but needs further evaluation in terms of its value in preventing recurrence.

  8. Aspectos epidemiológicos da leishmaniose tegumentar americana em Varzelândia, Minas Gerais, Brasil

    Adriana Guimarães Nunes

    Full Text Available Para caracterizar uma área endêmica de leishmaniose tegumentar americana susceptível de intervenção com candidato à vacina anti-leishmania, foi iniciado em 1999 estudo longitudinal em área rural do Município de Varzelândia, Minas Gerais, Brasil. Inicialmente, foram cadastrados 1.253 indivíduos distribuídos em 246 domicílios, dos quais, 1.170 concordaram em participar do trabalho. Desses, 593 (50,6% eram masculinos e 662 (56,5%, menores de 21 anos. A intradermorreação de Montenegro foi realizada em 1.120 indivíduos, lida em 1.020 dos casos, e observada reatividade em 282 (27,6% deles. Anticorpos anti-leishmania foram testados mediante imunofluorescência indireta e teste ELISA em 970 (82,9% indivíduos, obtendo-se positividade, respectivamente, em 127 (13,1% e 170 (17,5% para os dois testes. Na avaliação inicial, foram observados 297 (25,4% indivíduos com cicatrizes cutâneas semelhantes às deixadas pela doença. Desses, 282 realizaram a intradermorreação que foi positiva em168 (59,61%. A prevalência de casos de leishmaniose tegumentar no início do estudo foi de 5,8% e, depois do primeiro ano, observou-se incidência anual de 4,6% de casos. As características epidemiológicas observadas nesse local sugerem tratar-se de uma área endêmica de colonização antiga.

  9. Anticonvulsant and antioxidant effects of Tilia americana var. mexicana and flavonoids constituents in the pentylenetetrazole-induced seizures.

    Cárdenas-Rodríguez, Noemí; González-Trujano, María Eva; Aguirre-Hernández, Eva; Ruíz-García, Matilde; Sampieri, Aristides; Coballase-Urrutia, Elvia; Carmona-Aparicio, Liliana

    2014-01-01

    Tilia genus is commonly used around the world for its central nervous system properties; it is prepared as tea and used as tranquilizing, anticonvulsant, and analgesic. In this study, anticonvulsant activity of the Tilia americana var. mexicana inflorescences and leaves was investigated by evaluating organic and aqueous extracts (100, 300, and 600 mg/kg, i.p.) and some flavonoids in the pentylenetetrazole-induced seizures in mice. Moreover, antioxidant effect of these extracts and flavonoids was examined in an in vitro study by using spectrophotometric technique. Significant activity was observed in the methanol extract from inflorescences. An HPLC analysis of the methanol extract from inflorescences and leaves of Tilia allowed demonstrating the respective presence of some partial responsible flavonoid constituents: quercetin (20.09 ± 1.20 μg/mg and 3.39 ± 0.10 μg/mg), rutin (3.52 ± 0.21 μg/mg and 8.94 ± 0.45 μg/mg), and isoquercitrin (1.74 ± 0.01 μg/mg and 1.24 ± 0.13 μg/mg). In addition, significant but different antioxidant properties were obtained among the flavonoids and the extracts investigated. Our results provide evidence of the anticonvulsant activity of Tilia reinforcing its utility for central nervous system diseases whose mechanism of action might involve partial antioxidant effects due to the presence of flavonoids.

  10. Anticonvulsant and Antioxidant Effects of Tilia americana var. mexicana and Flavonoids Constituents in the Pentylenetetrazole-Induced Seizures

    Noemí Cárdenas-Rodríguez

    2014-01-01

    Full Text Available Tilia genus is commonly used around the world for its central nervous system properties; it is prepared as tea and used as tranquilizing, anticonvulsant, and analgesic. In this study, anticonvulsant activity of the Tilia americana var. mexicana inflorescences and leaves was investigated by evaluating organic and aqueous extracts (100, 300, and 600 mg/kg, i.p. and some flavonoids in the pentylenetetrazole-induced seizures in mice. Moreover, antioxidant effect of these extracts and flavonoids was examined in an in vitro study by using spectrophotometric technique. Significant activity was observed in the methanol extract from inflorescences. An HPLC analysis of the methanol extract from inflorescences and leaves of Tilia allowed demonstrating the respective presence of some partial responsible flavonoid constituents: quercetin (20.09 ± 1.20 μg/mg and 3.39 ± 0.10 μg/mg, rutin (3.52 ± 0.21 μg/mg and 8.94 ± 0.45 μg/mg, and isoquercitrin (1.74 ± 0.01 μg/mg and 1.24 ± 0.13 μg/mg. In addition, significant but different antioxidant properties were obtained among the flavonoids and the extracts investigated. Our results provide evidence of the anticonvulsant activity of Tilia reinforcing its utility for central nervous system diseases whose mechanism of action might involve partial antioxidant effects due to the presence of flavonoids.

  11. Anticonvulsant and Antioxidant Effects of Tilia americana var. mexicana and Flavonoids Constituents in the Pentylenetetrazole-Induced Seizures

    Cárdenas-Rodríguez, Noemí; González-Trujano, María Eva; Aguirre-Hernández, Eva; Ruíz-García, Matilde; Sampieri, Aristides; Coballase-Urrutia, Elvia; Carmona-Aparicio, Liliana

    2014-01-01

    Tilia genus is commonly used around the world for its central nervous system properties; it is prepared as tea and used as tranquilizing, anticonvulsant, and analgesic. In this study, anticonvulsant activity of the Tilia americana var. mexicana inflorescences and leaves was investigated by evaluating organic and aqueous extracts (100, 300, and 600 mg/kg, i.p.) and some flavonoids in the pentylenetetrazole-induced seizures in mice. Moreover, antioxidant effect of these extracts and flavonoids was examined in an in vitro study by using spectrophotometric technique. Significant activity was observed in the methanol extract from inflorescences. An HPLC analysis of the methanol extract from inflorescences and leaves of Tilia allowed demonstrating the respective presence of some partial responsible flavonoid constituents: quercetin (20.09 ± 1.20 μg/mg and 3.39 ± 0.10 μg/mg), rutin (3.52 ± 0.21 μg/mg and 8.94 ± 0.45 μg/mg), and isoquercitrin (1.74 ± 0.01 μg/mg and 1.24 ± 0.13 μg/mg). In addition, significant but different antioxidant properties were obtained among the flavonoids and the extracts investigated. Our results provide evidence of the anticonvulsant activity of Tilia reinforcing its utility for central nervous system diseases whose mechanism of action might involve partial antioxidant effects due to the presence of flavonoids. PMID:25197430

  12. Biodegradação e toxicidade de corantes têxteis e efluentes da Estação de Tratamento de Águas Residuárias de Americana, SP

    Priscila Maria Dellamatrice

    2006-01-01

    As indústrias têxteis da região de Americana geram grande quantidade de efluentes e resíduos sólidos com grande carga de corantes que são naturalmente de difícil degradação. Assim o objetivo do presente trabalho foi avaliar a possibilidade de biodegradação dos corantes têxteis RBBR, índigo e preto enxofre, e dos resíduos resultantes da Estação de Tratamento de Esgoto da cidade de Americana, SP, (DAE Americana), a qual trata 400 l s-1 de esgoto municipal junto com efluentes de 43 indústrias tê...

  13. Ações afirmativas na Educação Superior: o que acadêmicos brasileiros podem aprender da experiência americana = Affirmative action in Higher Education: what Brazilian academics can learn from the American experience

    Somers, Patricia

    2009-01-01

    Full Text Available Este artigo discute a história das ações afirmativas na educação dos Estados Unidos, da filosofia das ações afirmativas e do mérito do discurso para a educação, bem como reflete sobre a experiência americana. Refere-se, também, ao acesso à Educação Superior de jovens provenientes de classes minoritárias, brasileira e americana, examinado a importância da conexão da educação e da qualidade de vida. Chama atenção, ainda, para as relações que podem ser feitas pela educação brasileira, considerando a experiência americana

  14. Imagen de América Latina en "La Ilustración Española y Americana", en el siglo XIX

    Lic. Vivian Jara

    1998-01-01

    Full Text Available 'La Ilustración Española y Americana' es, probablemente, la más sobresaliente publicación semanal que se conserva de la época, una de las de mayor tirada y de mayor difusión, tanto en el Viejo como en el Nuevo Continente, y probablemente también una de las de mayor perdurabilidad en el tiempo. Se publicaba en Madrid y era distribuida a todos los países de habla hispana. Para realizar este trabajo la autora trabajó con los ejemplares de La Ilustración Española y Americana desde 1876 y hasta 1881, inclusive, y con los Almanaques de La Ilustración conservados en la Biblioteca de la Universidad de La Laguna. El trabajo consistió en el relevamiento de imágenes de y/o referidas a América Latina. Con tal fin y bajo la dirección y tutoría del profesor Adrián Alemán de Armas, se elaboró una ficha de investigación que condensó los aspectos esenciales de dichas imágenes. Asimismo, se realizaron simultáneamente observaciones acerca de algunos aspectos más sobresalientes, en los artículos referidos a América Latina. Estos son los primeros resultados de dichas observaciones. Por supuesto, aún queda mucho por releer, re-analizar y elaborar. En principio, hemos tratado de determinar, desde una perspectiva cuantitativa, el porcentual de espacio asignado a América Latina, en las páginas de La Ilustración Española y Americana.

  15. ALTERNATIVAS MICROBIOLÓGICAS PARA EL MANEJO DE Phytophthora cinnamomi Rands., EN Persea americana Mill. BAJO CONDICIONES DE CASA-MALLA

    Joaquín G. Ramírez Gil

    2014-01-01

    Full Text Available Para la producción de aguacate en Colombia es necesario establecer alternativas de manejo amigables con el medio ambiente y de bajo costo para el control de la enfermedad conocida como marchitez, cuyo principal agente causal es Phytophthora cinnamomi. El uso de microorganismos del suelo, es una opción viable para mejorar la nutrición y sanidad en Persea americana, buscando ser más competitivos en aras de afrontar los tratados de libre comercio y poder aprovechar el potencial exportador que presenta este frutal. Entendiendo la complejidad del sistema de la microbiota del suelo, consecuencia de su gran diversidad y de las distintas relaciones ecológicas que lo gobiernan, este trabajo estuvo encaminado a evaluar en condiciones de invernadero el efecto de cepas de Trichoderma sp. , Glomus fasciculatum y una cepa de Pseudomonas sp., en el desarrollo de plántulas de P. americana , sin inocular e inoculadas con P. cinnamomi. Los resultados encontrados en este trabajo sugieren que G. fasciculatum y Pseudomonas sp., solos o en combinación, favorecen el desarrollo de plántulas de aguacate, mientras que Trichoderma sp. presentó los mejores resultados en la reducción del progreso de la enfermedad de marchitez en plantas inoculadas con P. cinnamomi . Los microorganismos del suelo presentan un enorme potencial para el desarrollo y protección contra patógenos en P. americana , pero se hace necesario entender todas las relaciones para poder potenciar su uso como biofertilizantes y agentes de biocontrol.

  16. Study on Extraction Process of Periplaneta americana oil%美洲大蠊药材中油脂的提取工艺考察

    满红霞; 那凯歌; 谭巧云; 肖培云; 杨永寿

    2016-01-01

    目的:考察美洲大蠊药材中油脂的提取工艺以及皂化值的测定,为今后美洲大蠊药材中油脂的测定和开发利用提供依据。方法:以石油醚为溶剂,采用正交实验设计考察物料比、提取温度、提取时间3因素对油脂的提取率和皂化值的影响。结果:采用索氏提取法,物料比为1:15,温度80℃,时间2 h提取率最高。结论:验证性实验结果表明,该方法操作简便、结果准确,可作为美洲大蠊药材中油脂的提取方法。%〔Abstract〕Objective: Studying the extraction process of Periplaneta americana oil and determining the saponification value, to provide scientific basis for the future oil determination,exploitation and utilization. Methods:With petroleum ether as the solvent, the effects of extraction material ratio, temperature and time on the extraction rate and the saponification value were investigated by orthogonal experiment. Results: When using the method of cable extraction, the optimum conditions for Periplaneta americana lipid extraction were: extraction ratio of material to solvent: 1:15, temperature: 80 ℃, time: 2 h. Conclusion: Verification experimental results show that the method is simple and accurate, which can be used as a method for the extraction of Periplaneta americana oil.

  17. Tratamiento contable de las transacciones separadas en las combinaciones de negocios Proceso armonizador de la normativa contable internacional (IFRS) y americana (FAS)

    Montserrat Manzaneque Lizano; Elena Merino Madrid; Regino Banegas Ochovo

    2013-01-01

    Los cambios introducidos en la FAS 141(R) (2007) americana y en la IFRS 3 (R) (2008), con el fin de armonizar los aspectos contables de las operaciones referentes a la combinación de negocios, han puesto de manifiesto la existencia de determinadas transacciones que no forman parte de la propia combinación y a las que se denomina transacciones separadas, cuya delimitación de la combinación de negocios queda dispersa en ambas normas, por lo que en ocasiones resulta poco clarificador. Así, este ...

  18. Clasificación Sachs-Hornbostel de instrumentos musicales: una revisión y aplicación desde la perspectiva americana

    Pérez de Arce,José; Gili,Francisca

    2013-01-01

    El sistema de clasificación de instrumentos musicales ideado por Curt Sachs y Erich Moritz von Horbonstel en 1914 es universalmente usado hasta hoy. En este artículo se analizan las razones de esta permanencia y se revisa en general la historia de los distintos criterios usados para clasificar los instrumentos musicales. Igualmente se explica, de acuerdo con nuestra experiencia, el porqué el sistema de Sachs-Hornbostel nos parece el más apropiado para la realidad americana, con énfasis en la ...

  19. Estudos comportamentais de flebotomíneos (Diptera : Psychodidae : Phlebotominae) em área endêmica de Leishmaniose Tegumentar Americana no estado do Paraná, Brasil

    Gonçalves, André Luiz

    2014-01-01

    Orientadora : Profª Drª Edilene Alcântara de Castro Co-orientadora : Drª Nataly Araújo de Souza Tese (doutorado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Ciencias Biológicas (Microbiologia, Parasitologia e Patologia Básica). Defesa: Curitiba, 29/11/2014 Inclui referências : f. 75-108 Resumo: Algumas espécies de protozoários do gênero Leishmania Ross, 1903 são causadores da Leishmaniose Tegumentar Americana (LTA) no Novo Mundo, in...

  20. Obtención e identificación de barrenadores del tallo del aguacate (persea americana mill.) y de los hongos entomopatógenos asociados.

    Perales Rosas, Miguel Angel

    2016-01-01

    El aguacate (Persea americana Mill.) es una planta originaria de Mesoamérica. Actualmente, México es el primer productor y exportador mundial de este fruto. El barrenador del tallo del aguacate es una plaga que afecta a este cultivo y para su control se emplean plaguicidas químicos; una alternativa de manejo pueden ser los hongos entomopatógenos. Por lo anterior, el objetivo de este trabajo fue obtener e identificar insectos barrenadores del tallo de aguacate y los hongos entom...

  1. Leishmaniose tegumentar americana associada à AIDS: relato de quatro casos American cutaneous leishmaniasis associated with HIV/AIDS: report of four clinical cases

    Raimunda Nonata Ribeiro Sampaio; Cristina Paula Salaro; Paula Resende; Carmen Déa Ribeiro de Paula

    2002-01-01

    A co-infecção leishmaniose tegumentar americana e AIDS é de relato recente na literatura, observando-se diversidade quanto ao comportamento clínico e imunológico destes pacientes. Relatamos quatro casos acompanhados no Hospital Universitário de Brasília, com diagnóstico de infecção por parasitas do gênero Leishmania e pelo vírus da imunodeficiência humana, ilustrando diferentes apresentações clínicas, evoluções e respostas terapêuticas.The co-infection American cutaneous leishmaniasis and AID...

  2. Efeito do cultivo orgânico e convencional sobre a vida-de-prateleira de alface americana (Lactuca sativa L.) minimamente processada

    Mello Júlio César; Dietrich Renato; Meinert Elza Maria; Teixeira Evanilda; Amante Edna Regina

    2003-01-01

    A alface americana (Lactuca sativa), minimamente processada, foi avaliada quanto aos efeitos dos diferentes sistemas de cultivo (orgânico e convencional), sobre a vida-de-prateleira. A alface minimamente processada foi embalada em sacos de polietileno de baixa densidade (PEBD), armazenada em refrigerador a 4masculineC e avaliada até o final da vida-de-prateleira, estabelecida por métodos sensoriais. As avaliações foram realizadas durante a estocagem, nas condições pré-estabelecidas. Nos tempo...

  3. The taruca (Hippocamelus antisensis) and the red brocket deer (Mazama americana) as intermediate hosts of Taenia hydatigena in Peru, morphological and molecular evidence.

    Gomez-Puerta, Luis A; Pacheco, Joel; Gonzales-Viera, Omar; Lopez-Urbina, Maria T; Gonzalez, Armando E

    2015-09-15

    In the present report metacestodes were collected from the mesentery of a taruca (Hippocamelus antisensis) and from the omentum of a red brocket deer (Mazama americana) in Peru. Various metacestodes parameters, including rostellar hook characteristics, were measured. Molecular analysis was performed to amplify the mitochondrial cytochrome c oxidase subunit 1 gene from metacestode isolates. Metacestodes were identified as T. hydatigena by morphology and molecular methods. This constitutes the first molecular detection of T. hydatigena metacestodes in the taruca and the red brocket deer and demonstrates that these animal species are natural intermediate hosts for this parasite.

  4. Clima social escolar y rendimiento académico en los niños de sexto grado del Colegio Adventista Americana De Juliaca, 2014

    Huanca Cutipa, Maritza

    2014-01-01

    Un apropiado clima social escolar es muy importante para que haya un buen aprendizaje, el presente estudio tiene el objetivo de establecer la relación entre el clima social escolar y el rendimiento académico en los estudiantes de sexto grado de primaria del Colegio Adventista Americana de Juliaca en el año 2014. Es un estudio no experimental, con un tipo descriptivo, correlacional, transeccional, se trabajó con una muestra de 47 niños de sexto grado de las secciones A y B...

  5. La revolución Americana: una revuelta desde y contra Inglaterra. Un ensayo sobre sus orígenes ideológicos.

    García Portela, Laura

    2014-01-01

    La Revolución Americana constituye un hito en la Historia contemporánea occidental cuyo modelo institucional ha ejercido una influencia que llega hasta nuestros días, pues pasa por ser la primera democracia contemporánea. Este ensayo indaga en las corrientes ideológicas que se mezclaron en el discurso de los revolucionarios, con la intención de ponderar las diferentes fuentes empleadas por ellos: el pensamiento de raíz religiosa del “Gran Despertar”, la Ilustración francesa y el pensamiento r...

  6. Leishmaniose tegumentar americana: estudo clínico, epidemiológico e laboratorial realizado no Hospital Universitário de Campo Grande, Mato Grosso do Sul, Brasil

    Murback, Nathalia Dias Negrão; Hans Filho, Günter; Nascimento,Roberta Ayres Ferreira do; Nakazato, Katia Regina de Oliveira; Dorval, Maria Elizabeth Moraes Cavalheiros

    2011-01-01

    FUNDAMENTOS: Leishmaniose Tegumentar Americana é zoonose de manifestações clínicas variadas, em expansão no Brasil, sendo o estado de Mato Grosso do Sul importante área endêmica. OBJETIVOS: Avaliar clínica, epidemiológica e laboratorialmente pacientes com Leishmaniose Tegumentar Americana , atendidos no Hospital Universitário Maria Aparecida Pedrossian, Universidade Federal de Mato Grosso do Sul, Brasil (HU/UFMS). MÉTODOS: Trata-se de um estudo observacional do tipo transversal com abor...

  7. A crise financeira americana de 2008: um exame comparativo da explicação da Hipótese da Instabilidade Financeira de Minsky e da Teoria Austríaca do Ciclo Econômico

    Mendes, Lucas

    2016-01-01

    O objetivo deste estudo é realizar uma análise comparativa entre as teorias do economista americano Hyman Minsky e da Teoria Austríaca do Ciclo Econômico (TACE), em relação ao seu poder explicativo para a grande crise financeira americana de 2007-8, inaugurada com o estouro de uma bolha imobiliária. Com esse objetivo em mente, iniciamos descrevendo um conjunto de variáveis políticas e econômicas relevantes que conduziram a economia americana à grande crise. Feito isso, expomos a Hipótese da I...

  8. Consumo de alimentos dos grupos que compõem a pirâmide alimentar americana por idosos brasileiros: uma revisão = Food intake of the groups part of the American food pyramid by Brazilian elderly: a review

    Deon, Rúbia Garcia

    2015-01-01

    Introdução: A pirâmide alimentar adaptada para a população idosa americana é um guia alimentar que destaca a importância da alimentação equilibrada para o envelhecimento bem sucedido Objetivo: Apresentar uma revisão literária sobre as evidências do consumo de alimentos dos grupos que compõem a pirâmide alimentar americana por idosos brasileiros Materiais e M étodos: Foram incluídos artigos científicos (identificados no LILACS e Medline/PubMed), dissertações e teses (identificadas no por...

  9. Euforia e pessimismo: os ciclos de ativos, crédito e investimento da economia americana após 1982

    Marcos Antonio Cintra

    2007-11-01

    Full Text Available A partir da década de 1980, as finanças americanas passaram por profundas transformações com o predomínio crescente dos mercados de capitais (market led finance. A forma de gestão da riqueza e do sistema de crédito infligiu dinâmica específica ao preço dos ativos, com repercussões nas decisões de gasto (consumo e investimento dos agentes econômicos. Busca-se, aqui, discutir a evolução patrimonial desses agentes nos últimos três ciclos de crescimento da economia americana, sob o comando dos ciclos de ativos e de crédito, chamados financial led.The US finance underwent deep changes as from the 1980s when market led finance increasingly prevailed. The wealth and credit management forced a specific dynamics upon asset prices and reverberated throughout spending decisions (consumption and investment of economic agents. This paper deals with the net worth evolution of these agents in the recent three growth cycles of the US economy under the command of asset and credit cycles, the so-called financial-led cycles.

  10. Analysis of expressed sequence tags (ESTs) from avocado seed (Persea americana var. drymifolia) reveals abundant expression of the gene encoding the antimicrobial peptide snakin.

    Guzmán-Rodríguez, Jaquelina J; Ibarra-Laclette, Enrique; Herrera-Estrella, Luis; Ochoa-Zarzosa, Alejandra; Suárez-Rodríguez, Luis María; Rodríguez-Zapata, Luis C; Salgado-Garciglia, Rafael; Jimenez-Moraila, Beatriz; López-Meza, Joel E; López-Gómez, Rodolfo

    2013-09-01

    Avocado is one of the most important fruits in the world. Avocado "native mexicano" (Persea americana var. drymifolia) seeds are widely used in the propagation of this plant and are the primary source of rootstocks globally for a variety of avocado cultivars, such as the Hass avocado. Here, we report the isolation of 5005 ESTs from the 5' ends of P. americana var. drymifolia seed cDNA clones representing 1584 possible unigenes. These avocado seed ESTs were compared with the avocado flower EST library, and we detected several genes that are expressed either in both tissues or only in the seed. The snakin gene, which encodes an element of the innate immune response in plants, was one of those most frequently found among the seed ESTs, and this suggests that it is abundantly expressed in the avocado seed. We expressed the snakin gene in a heterologous system, namely the bovine endothelial cell line BVE-E6E7. Conditioned media from transfected BVE-E6E7 cells showed antimicrobial activity against strains of Escherichia coli and Staphylococcus aureus. This is the first study of the function of the snakin gene in plant seed tissue, and our observations suggest that this gene might play a protective role in the avocado seed.

  11. Effect of hydroalcoholic fruit extract of Persea americana Mill. on high fat diet induced obesity: A dose response study in rats.

    Monika, Padmanabhan; Geetha, Arumugam

    2016-06-01

    The fruits of Persea Americana Mill., commonly known as Avocado, are traditionally consumed for various health benefits including weight reduction. Here, we studied the effect of hydroalcoholic fruit extract of Persea americana (HAEPA) on high fat diet (HFD) induced obesity in rats. Obesity was induced in male Sprague Dawley rats by feeding HFD for 14 wk. The hypolipidemic effect was evaluated by co-administering 25, 50, 100 and 200 mg/kg body wt. of HAEPA. There was a significant increase in weight gain, body mass index (BMI), blood lipids, low density lipoproteins (LDL), lipid peroxides (LPO) and serum transaminases in HFD fed rats. HFD+HAEPA fed rats showed a significant decrease in blood lipids, LPO, liver lipids and increase in antioxidant status when compared to HFD control rats. The activity of lipid metabolic key enzymes such as fatty acid synthase and HMG CoA reductase in liver were also found to be decreased significantly in HAEPA co-administered rats. Lipoprotein lipase activity was found increased in HFD+HAEPA rats. Among the 4 doses studied, 100 mg of HAEPA/kg body wt. exhibited optimum hypolipidemic activity. Histopathological observations in liver and visceral adipose tissue added more evidence for the lipid lowering effect of HAEPA. It can be concluded that avocado fruit extract can act as hypolipidemic agent probably by modulating the activities of HMG CoA reductase and fatty acid synthase in liver.

  12. 龙舌兰发酵叶汁中的一个新甾体皂苷%A New Steroidal Saponin from Fermented Leaves of Agave americana

    金建明; 刘锡葵; 杨崇仁

    2002-01-01

    A new steroidal saponin and two known steroidal comp ounds were isolated from fermented leaves of Agave americana.The structure o f the new steroidal saponin was elucidated as tigogenin 3-O-α-L-rhamnopyran osyl-(1→3)-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→ 3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside by spectros copic data and chemical method.%从龙舌兰(Agave americana)的发酵叶汁中分离到一个新甾体皂苷.结合波谱和化学方法,新化合物的结构鉴定为替告皂苷元3-O-α-L-鼠李吡喃糖基-(1→3)-β-D-葡萄吡喃糖基-(1→2)-[β-D-葡萄吡喃糖基-(1→3)]-β-D-葡萄吡喃糖基-(1→4)-β-D-半乳吡喃糖苷.

  13. Green synthesis, characterisation and biological evaluation of AgNPs using Agave americana, Mentha spicata and Mangifera indica aqueous leaves extract.

    Ahmad, Bashir; Shireen, Farah; Bashir, Shumaila; Khan, Ibrar; Azam, Sadiq

    2016-10-01

    The current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (AgNPs) of Agave americana, Mentha spicata and Mangifera indica leaves and to screen them for biological activities. The ultraviolet-visible spectroscopic analysis revealed that λ-max for AgNPs range from 350-500 nm. All AgNPs possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in X-ray diffraction measurements and supported by scanning electron microscopy data. The size of the nano-particles was confirmed by transmission electron microscopy (30-150 nm). Mass loss at variable temperatures was evaluated by simultaneous thermogravimetric and differential thermal analysis revealed reduction in mass and activity of compounds was notified by temperature increase from 200 to 800 °C, thus concluding it as thermally sensitive compounds. A. americana AgNPs showed significant (96%) activity against Methicillin resistant Staphylococcus aureus, Escherichia coli (95%) and Fusarium oxysporum (89%). Good antioxidant activity was shown by M. spicata AgNPs at 300 µl (79%). M. indica AgNPs showed significant phytotoxic activity (88%) at highest concentration. No haemagglutination reaction was observed for the test samples. The above results revealed that AgNPs synthesized from selected plant species possesses significant antimicrobial and phytotoxic effect.

  14. Hematological and morphometric differences of blood cells from rheas, Rhea americana (Struthioniformes: Rheidae on two conservation farms

    S. S. M. Gallo

    Full Text Available Abstract Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espírito Santo (ES, and the other in São Carlos, São Paulo (SP, were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained. The results from the two farms analyzed were compared by means of the t test. There were differences in the parameters of the erythrocyte series between the two flocks because of a process of hypochromic macrocytic anemia observed among the rheas in ES. The values for the erythrocyte series and indexes were: RBC, 2.81 ± 0.15 x106/μl; PCV, 44.20 ± 2.86%; Hb, 12.12 ± 0.74 g/dL; MCV, 15.75 ± 0.89 fL; MCH, 43.18 ± 1.82 pg; and MCHC, 27.44 ± 0.80 g/dL. The values for the leukocyte series were: WBC, 12,072 ± 4116 /μL; heterophils, 64.10 ± 9.90%; eosinophils, 2.05 ± 2.06%; monocytes, 6.40 ± 2.99%; lymphocytes, 26.93 ± 9.62%; and basophils, 0.52 ± 1.27%. These can all be suggested as references for rheas reared in Brazil. Statistical differences were observed in erythrocyte length measurements, which were higher in the rheas in SP than in those in ES. Regarding width, the heterophils, lymphocytes and monocytes of the birds in ES were statistically greater than those of the birds in SP. It was concluded that rheas may exhibit morphometric alterations to blood cells and differences in blood elements according to the type of management to which they are subjected.

  15. Inhibition of phenylpropanoid biosynthesis increases cell wall digestibility, protoplast isolation, and facilitates sustained cell division in American elm (Ulmus americana

    Jones A Maxwell P

    2012-05-01

    Full Text Available Abstract Background Protoplast technologies offer unique opportunities for fundamental research and to develop novel germplasm through somatic hybridization, organelle transfer, protoclonal variation, and direct insertion of DNA. Applying protoplast technologies to develop Dutch elm disease resistant American elms (Ulmus americana L. was proposed over 30 years ago, but has not been achieved. A primary factor restricting protoplast technology to American elm is the resistance of the cell walls to enzymatic degradation and a long lag phase prior to cell wall re-synthesis and cell division. Results This study suggests that resistance to enzymatic degradation in American elm was due to water soluble phenylpropanoids. Incubating tobacco (Nicotiana tabacum L. leaf tissue, an easily digestible species, in aqueous elm extract inhibits cell wall digestion in a dose dependent manner. This can be mimicked by p-coumaric or ferulic acid, phenylpropanoids known to re-enforce cell walls. Culturing American elm tissue in the presence of 2-aminoindane-2-phosphonic acid (AIP; 10-150 μM, an inhibitor of phenylalanine ammonia lyase (PAL, reduced flavonoid content, decreased tissue browning, and increased isolation rates significantly from 11.8% (±3.27 in controls to 65.3% (±4.60. Protoplasts isolated from callus grown in 100 μM AIP developed cell walls by day 2, had a division rate of 28.5% (±3.59 by day 6, and proliferated into callus by day 14. Heterokaryons were successfully produced using electrofusion and fused protoplasts remained viable when embedded in agarose. Conclusions This study describes a novel approach of modifying phenylpropanoid biosynthesis to facilitate efficient protoplast isolation which has historically been problematic for American elm. This isolation system has facilitated recovery of viable protoplasts capable of rapid cell wall re-synthesis and sustained cell division to form callus. Further, isolated protoplasts survived

  16. Polyuronides in Avocado (Persea americana) and Tomato (Lycopersicon esculentum) Fruits Exhibit Markedly Different Patterns of Molecular Weight Downshifts during Ripening.

    Huber, D. J.; O'Donoghue, E. M.

    1993-01-01

    Avocado (Persea americana) fruit experience a rapid and extensive loss of firmness during ripening. In this study, we examined whether the chelator solubility and molecular weight of avocado polyuronides paralleled the accumulation of polygalacturonase (PG) activity and loss in fruit firmness. Polyuronides were derived from ethanolic precipitates of avocado mesocarp prepared using a procedure to rapidly inactivate endogenous enzymes. During ripening, chelator (cyclohexane-trans-1,2-diamine tetraacetic acid [CDTA])-soluble polyuronides increased from approximately 30 to 40 [mu]g of galacturonic acid equivalents (mg alcohol-insoluble solids)-1 in preripe fruit to 150 to 170 [mu]g mg-1 in postclimacteric fruit. In preripe fruit, chelator-extractable polyuronides were of high molecular weight and were partially excluded from Sepharose CL- 2B-300 gel filtration media. Avocado polyuronides exhibited marked downshifts in molecular weight during ripening. At the postclimacteric stage, nearly all chelator-extractable polyuronides, which constituted from 75 to 90% of total cell wall uronic acid content, eluted near the total volume of the filtration media. Rechromatography of low molecular weight polyuronides on Bio-Gel P-4 disclosed that oligomeric uronic acids are produced in vivo during avocado ripening. The gel filtration behavior and pattern of depolymerization of avocado polyuronides were not influenced by the polyuronide extraction protocol (imidazole versus CDTA) or by chromatographic conditions designed to minimize interpolymeric aggregation. Polyuronides from ripening tomato (Lycopersicon esculentum) fruit extracted and chromatographed under conditions identical with those used for avocado polyuronides exhibited markedly less rapid and less extensive downshifts in molecular weight during the transition from mature-green to fully ripe. Even during a 9-d period beyond the fully ripe stage, tomato fruit polyuronides exhibited limited additional depolymerization and

  17. Epizootic vacuolar myelinopathy of the central nervous system of bald eagles (Haliaeetus leucocephalus) and American coots (Fulica americana)

    Thomas, N.J.; Meteyer, C.U.; Sileo, L.

    1998-01-01

    Unprecedented mortality occurred in bald eagles (Haliaeetus leucocephalus) at DeGray Lake, Arkansas, during the winters of 1994-1995 and 1996-1997. The first eagles were found dead during November, soon after arrival from fall migration, and deaths continued into January during both episodes. In total, 29 eagles died at or near DeGray Lake in the winter of 1994-1995 and 26 died in the winter of 1996-1997; no eagle mortality was noted during the same months of the intervening winter or in the earlier history of the lake. During the mortality events, sick eagles were observed overflying perches or colliding with rock walls. Signs of incoordination and limb paresis were also observed in American coots (Fulica americana) during the episodes of eagle mortality, but mortality in coots was minimal. No consistent abnormalities were seen on gross necropsy of either species. No microscopic findings in organs other than the central nervous system (CNS) could explain the cause of death. By light microscopy, all 26 eagles examined and 62/77 (81%) coots had striking, diffuse, spongy degeneration of the white matter of the CNS. Vacuolation occurred in all myelinated CNS tissue, including the cerebellar folia and medulla oblongata, but was most prominent in the optic tectum. In the spinal cord, vacuoles were concentrated near the gray matter, and occasional swollen axons were seen. Vacuoles were uniformly present in optic nerves but were not evident in the retina or peripheral or autonomic nerves. Cellular inflammatory response to the lesion was distinctly lacking. Vacuoles were 8-50 microns in diameter and occurred individually, in clusters, or in rows. In sections stained by luxol fast blue/periodic acid-Schiff stain, the vacuoles were delimited and transected by myelin strands. Transmission electron microscopy revealed intramyelinic vacuoles formed in the myelin sheaths by splitting of one or more myelin lamellae at the intraperiodic line. This lesion is characteristic of

  18. Consumo de alimentos dos grupos que compõem a pirâmide alimentar americana por idosos brasileiros: uma revisão = Food intake of the groups part of the American food pyramid by Brazilian elderly: a review

    Deon, Rúbia Garcia

    2015-01-01

    Conclusão: As evidências apontam que o consumo, por idosos brasileiros, dos alimentos integrantes dos grupos da pirâmide alimentar adaptada para a população idosa americana, em geral, não está adequado

  19. Incidence and growth of Salmonella enterica on the peel and pulp of avocado (Persea americana) and custard apple (Annona squamosa).

    Rezende, Ana Carolina B; Crucello, Juliana; Moreira, Rafael C; Silva, Beatriz S; Sant'Ana, Anderson S

    2016-10-17

    The aim of this study was to assess the incidence and to estimate the growth kinetic parameters (maximum growth rate, μ; lag time, λ; and maximum population, κ) of Salmonella on the peel and pulp of avocado (Perseaamericana var. americana) and custard apple (Annona squamosa L.) as affected by temperature (10-30°C). The incidence of Salmonella was assessed on the peel and pulp of the fruits (n=200 of each fruit), separately, totalizing 800 analyses. Only three samples of custard apple pulp were positive for Salmonella enterica and the three isolates recovered belonged to serotype S. Typhimurium. Salmonella was not recovered from avocado and custard apple peels and from avocado pulp. Generally, the substrate (pulp or peel) of growth did not affect μ values of S. enterica (p>0.05). Very similar μ values were found for S. enterica inoculated in custard apple and avocado. S. enterica presented the highest λ in the peel of the fruits. The growth of S. enterica resulted in larger λ in custard apple in comparison to avocado. For example, the λ of S. enterica in the pulp of custard apple and avocado were 47.0±0.78h and 10.0±3.78h, respectively. The lowest values of κ were obtained at the lower storage temperature conditions (10°C). For instance, κ values of 3.7±0.06log CFU/g and 2.9±0.03log CFU/g were obtained from the growth of S. enterica in avocado and custard apple pulps at 10°C (p<0.05), respectively. On the other hand, at 30°C, κ values were 6.5±0.25log CFU/g and 6.5±0.05log CFU/g, respectively. Significantly higher κ were obtained from the growth of S. enterica in the pulp than in the peel of the fruits (p<0.05). For instance, the growth of S. enterica in the pulp of avocado led to a κ value of 6.5±0.25log CFU/g, while in the peel led to a κ value of 4.6±0.23log CFU/g (p<0.05). In general, growth kinetic parameters indicated that avocado comprises a better substrate than custard apple for the growth of S. enterica. The square root model

  20. Immunocytochemical demonstration of proopiomelanocortin- and other opioid-related substances and a CRF-like peptide in the gut of the american cockroach, Periplaneta americana L.

    Schols, D; Verhaert, P; Huybrechts, R; Vaudry, H; Jégou, S; De Loof, A

    1987-01-01

    Using the peroxidase-antiperoxidase technique, we showed the presence of peptides which are immunologically resembling mammalian corticotropin releasing hormone (CRF)-, adrenocorticotropic hormone (ACTH)-, beta-endorphin (beta-END)-, alpha-melanocyte stimulating hormone (alpha-MSH)-, methionine-enkephalin (met-ENK)- and leucine enkephalin (leu-ENK)- like immunoreactivity in hundreds to thousands of endocrine cells and nerve fibers in the midgut of the American cockroach Periplaneta americana. In the cockroach hindgut no immunoreactive cell bodies could be observed, although nerve fibers were clearly noticed to be recognized by antisera to CRF, ACTH1-24, ACTH11-24 and beta-END. Nothing is exactly known as to the function(s) of the demonstrated materials, but one can speculate that these numerous immunoreactive cells, might have important paracrine and/or endocrine functions in the insect physiology.

  1. Comparação dos procedimentos de "imprint " e escarificação no diagnóstico da Leishmaniose Tegumentar Americana

    Mello, Cintia Xavier de

    2011-01-01

    A leishmaniose tegumentar americana (LTA) é uma doença infecciosa, causada por parasitas do gênero Leishmania, que apresenta características complexas em diferentes aspectos. O diagnóstico, sempre que possível, deve ser feito com base em evidências epidemiológicas, aspecto clínico e exames laboratoriais. Para pesquisa direta do parasito, são utilizados os procedimentos de escarificação e “imprint”, sendo a escarificação o método mais rápido, de menor custo e de fácil execução. ...

  2. Descripción de Thelastoma domesticus sp. nov. (Oxyurida, Thelastomatidae parásita de ninfas Periplaneta americana (Blattodea, Blattidae en Argentina

    Nora B. Camino

    2008-03-01

    Full Text Available Thelastoma domesticus sp. nov. se halló parasitando a ninfas de Periplaneta americana (Linnaeus, 1758 en la ciudad de La Plata, Província de Buenos Aires, Argentina, en el presente trabajo se describe e ilustra. Esta especie nueva se caracteriza por tener en la boca un estoma simple de paredes gruesas, fuertemente cuticularizada formando tres pares de nodulos en dos filas, sin dientes, el poro excretor es posterior, se ubica al comienzo del intestino, el macho presenta una distribución particular de las papilas genitales: un par grande de papilas preanales y tres pares posanales agrupadas, de las cuales el segundo par se encuentra desplazado lateralmente, configurando así con las otras papilas posanales un círculo.

  3. Leishmaniose tegumentar americana na Ilha Grande. Rio de Janeiro: V. Observações sobre a biologia dos transmissores em condiçoes naturais

    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Durante um surto de Leishmaniose Tegumentar Americana (LTA na Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, foram coletados 4.192 exemplares de flebotomíneos em ambiente domiciliar e silvestre, com isca animal e outros abrigos naturais, durante o período de janeiro de 1976 a abril de 1977. Entre o total de 11 espécies coletadas encontraram-se 2.493 exemplares de Lutzomyia intermedia, 1.594 de Lutzomyia migonei e 105 exemplares de outras espécies. A L. intermedia apresentou densidade mais elevada no domicílio humano, enquanto a L. migonei apresentou coletas mais rendosas com iscas animais e galinheiros. O sinantropismo e a elevada densidade no ambiente domiciliar incriminam as espécies L. intermedia e L. migonei como as prováveis vetoras da LTA na Ilha Grande.

  4. Trypanocidal constituents in plants 5. Evaluation of some Mexican plants for their trypanocidal activity and active constituents in the seeds of Persea americana.

    Abe, Fumiko; Nagafuji, Shinya; Okawa, Masafumi; Kinjo, Junei; Akahane, Hiroshige; Ogura, Tetsuya; Martinez-Alfaro, Miguel Angel; Reyes-Chilpa, Ricardo

    2005-07-01

    Crude extracts of Mexican medicinal plants were screened for trypanocidal activity against Trypanosoma cruzi, which is the etiological agent for Chagas' disease, one of the most serious protozoan diseases in Latin America. There were 71 kinds of methanolic and other organic extracts from 65 plants, which were newly examined by a preliminary screening test to observe immobilization of epimastigotes and trypomastigotes of T. cruzi in vitro. The MeOH extract of seeds of Persea americana (avocado) showed moderate activity against epimastigotes. In order to identify the principal compounds for the activity, the MeOH extract was subjected to bioassay-guided fractionation. From the active fractions, six 1,2,4-trihydroxyheptadecane derivatives and two 1,2,4-trihydroxynonadecane derivatives including a new one were isolated. These compounds showed moderate activity against epimastigotes and trypomastigotes.

  5. Aprendendo com os erros dos outros: o que a história da ciência política americana tem para nos contar

    Feres Jr. João

    2000-01-01

    Este artigo pretende mostrar que a análise da história da Ciência Política americana pode nos ajudar a pensar criticamente as Ciências Sociais no Brasil. A versão historiográfica dominante, representada por John Gunnell e outros, ignora o papel ideológico e antidemocrático que a Ciência Política behaviorista assumiu durante a Guerra Fria, enquanto histórias mais críticas são mantidas à margem da disciplina. Essa situação espelha o arranjo institucional da própria Ciência Política, que promove...

  6. Inquérito sorológico sobre leishmaniose tegumentar americana em cães errantes no Estado de São Paulo, Brasil

    Elisa San Martin M Savani

    1999-12-01

    Full Text Available Inquérito sorológico para leishmaniose tegumentar americana em 973 cães errantes, capturados próximos a áreas verdes do Município de São Paulo, por meio da reação de imunofluorescência indireta, não demonstrou soros reagentes. No entanto, casos humanos autóctones dessa zoonose ocorridos no município indicam a circulação do parasita no ambiente. A existência de vetores e a aparente ausência de cães infectados nas áreas pesquisadas sugerem que o cão errante desempenhe papel irrelevante na disseminação do protozoário.

  7. Apropiación y alienación: consideraciones hermenéutico-existenciales a propósito de la Pastoral americana de Philip Roth

    Germán Darío Vélez López

    2013-01-01

    Full Text Available Partiendo de una formulación básica de algunos elementos relevantes de la hermenéutica fenomenológica de Heidegger, el presente artículo propone una lectura de la obra del escritor norteamericano Philip Roth titulada Pastoral americana, que lo hizo acreedor al premio Pulitzer en 1989. El propósito cen- tral de esa lectura consiste en mostrar de qué manera fenómenos existenciales elementales como estar-lejos (Wegsein, estar-despierto (Wachsein, estar-ahí (Dasein permiten una articulación propia del sentido de la obra de Roth en la que ésta manifiesta su particular compromiso existencial.

  8. La Unión Médica Hispano-Americana (1900) y su contribución al internacionalismo científico

    Olagüe de Ros, Guillermo

    2006-01-01

    En 1900, y en el curso del XIII Congreso Internacional de Medicina de París, se fundó la Unión Médica Hispano-Americana, cuyo objetivo básico era reforzar los lazos culturales y científicos entre los países de habla española. La primera asamblea de la Unión se celebró en Madrid en la primavera de 1903. De las comunicaciones presentadas la más interesante, sin duda, fue la Manuel Latosa Latour, que propuso la confección de un Diccionario Tecnológico Médico Hispano-Americano. Ni la Sociedad cie...

  9. Fruit flies (Diptera: Tephritidae associated to native fruit of Spondias spp. (Anacardiaceae and Ximenia americana L. (Olacaceae and their parasitoids in the State of Piaui, Brazil

    Almerinda Amélia Rodrigues Araújo

    2014-09-01

    Full Text Available This work aims to identify the species of fruit flies and their parasitoids associated to native fruit of Spondias spp. (caja S. mombin L., umbu-caja Spondias sp., umbu S. tuberosa Arr. Câm. and wild plum Ximenia americana L., in the State of Piaui, Brazil. Samples (63 of fruits were collected from November 2009 to July 2010, totalizing 4,495 fruits and 46,906 kg. It was possible to obtain 10,617 puparia, from which 4,497 tephritids and 1,118 braconid parasitoids emerged. Regarding Spondias spp., the highest occurrence was Anastrepha obliqua (Macquart, with 100% for umbu and umbu-caja. Caja presented an average of 99.52% of A. obliqua, 0.46% of Anastrepha fraterculus (Wied. and 0.97% of Ceratitis capitata (Wied.. Wild plum percentages were 97.83% for A. alveata Stone and 2.17% for A. fraterculus. Infestation rates were 429.2, 178.4, 158.9 and 43.3 puparia/kg in umbu-caja, caja, wild plum and umbu, respectively. Pupal viability was 77.8%, 69.3%, 52.5% and 41.1% to umbu, wild plum, umbu-caja and caja, respectively. By analyzing the sample parasitoids, the percentage was 21.39% for the Doryctobracon areolatus (Szépligeti species and 78.61% for Opius bellus Gahan. For the first time, it was recorded in Brazil X. americana as a host to A. alveata, as well as D. aleolatus and O. bellus as parasitoids of A. obliqua and A. alveata in Piaui.

  10. Bioactivity-guided isolation of beta-sitosterol and some fatty acids as active compounds in the anxiolytic and sedative effects of Tilia americana var. mexicana.

    Aguirre-Hernández, Eva; Rosas-Acevedo, Hortensia; Soto-Hernández, Marcos; Martínez, Ana Laura; Moreno, Julia; González-Trujano, Ma Eva

    2007-09-01

    Tilia species have been used as anxiolytics for many years. In a previous study anxiolytic-like effects of a hexane extract of Tilia americana var. mexicana inflorescences were observed in experimental models in mice. To get additional insights into the neuroactive actions of this particular Tilia species, in this study we report a bioactivity guided-fractionation of the extract and separation by column chromatographic methods to isolate three fatty acids and a triterpene identified as beta-sitosterol as major constituents. Our results revealed that the crude extract at 10 and 30 mg/kg I. P. and some pooled fractions at the same dosages potentiated sodium pentobarbital-induced sleeping time and caused a significant increase in the time spent at the open-arm sides in the plus-maze test. A reduction in the exploratory behavioral pattern manifested as ambulatory activity, as well as head dipping and rearing tests was also observed. Further fractionation and purification yielded four major fractions containing fatty acids and beta-sitosterol as the active compounds. A dose-response curve of beta-sitosterol in the range 1 to 30 mg/kg doses indicated that this compound produced an anxiolytic-like action from 1 to 10 mg/kg and a sedative response when the dose was increased to 30 mg/kg, these effects resemble those produced by diazepam (0.1 mg/kg). Our results suggest that hexane extract of Tilia americana var. mexicana produces depressant actions on the central nervous system, at least in part, because of the presence of beta-sitosterol and some fatty acids that remain to be identified.

  11. EL CONTEXTO, LAS TÉCNICAS Y LAS CONSECUENCIAS DE LA INTERPRETACIÓN DE LA CONVENCIÓN AMERICANA DE LOS DERECHOS HUMANOS

    Laurence Burgorgue-Larsen

    2014-01-01

    Full Text Available Desde su creación, la Corte Interamericana siempre ha privilegiado la finalidad pro- homine de la Convención Americana sobre Derechos Humanos. Para entender este fenómeno que le permite interpretar su texto de referencia de manera constructiva, este artículo examina el contexto en el cual ésta evoluciona. Por ende, después de haber introducido el tema (I Parte, los contextos jurídicos (el contenido de la Convención como tal, pero también político (el estado de la violencia instituciona- lizada en América Latina y sociológico (la composición de la Corte están descritos para entender las razones que pusieron, muy temprano, la Corte Interamericana a privilegiar de manera regular, cuando no sistemática, la interpretación más protectora de la Convención (II Parte. Una vez presentado el panorama general del contexto al cual la Corte está confrontada, son las técnicas de interpretación como tales las que están presentadas. Tienen como objeto interpretar los derechos protegidos de manera extensiva y tienen como consecuencias ampliar el ámbito de aplicación de la Convención Americana (III Parte. Son, al final del análisis, las consecuencias de tal metodología las que están analizadas tanto al nivel político (la reacción de unos cuantos Estados como al nivel jurídico e institucional (la recepción del control de convencionalidad en los Estados Parte y el aumento de las disidencias en el propio seno de la Corte (IV Parte.

  12. La exposición histórico-americana de Madrid de 1892 y la ¿ausencia? de México

    Ramírez Losada, Dení

    2009-08-01

    Full Text Available The Fourth Centennial of the Discovery of America was celebrated in 1892. Together with the rest of the festivities, the Historical American Exposition of Madrid is considered a landmark not only for its political, cultural and scientific relevance, which, to this day, have not been studied in their entirety, but as well for the implications that resulted for Mexico. This article is meant to show, on the one side, the singularity of such festivities, and, on the other, the fact that Mexico’s participation constituted an important historical moment for Mexican scientific anthropology. This research was essentially carried out based on the numerous documentation found at the Archivo General de la Administración, Alcalá de Henares.

    En 1892 se celebró en España el IV Centenario del Descubrimiento de América. En el marco de este festejo destacó, sin duda, la Exposición Histórico Americana de Madrid no sólo por su enorme relevancia política, cultural y científica que, hasta ahora, se ha estudiado poco, sino por todo aquello que significó para México en particular. El artículo centra su interés en destacar, por un lado, la singularidad de los festejos colombinos y, por el otro, mostrar que la participación de México en la Exposición Histórico-Americana constituyó todo un hito científico en la antropología mexicana. La investigación se llevó a cabo, fundamentalmente, sobre la copiosa documentación encontrada en el Archivo General de la Administración, Alcalá de Henares.

  13. Isolation, characterization, and localization of AgaSGNH cDNA: a new SGNH-motif plant hydrolase specific to Agave americana L. leaf epidermis.

    Reina, José J; Guerrero, Consuelo; Heredia, Antonio

    2007-01-01

    GDSL and SGNH hydrolases are lipases involved in a wide range of functions, behaving in many cases as bifunctional enzymes. In this work, the isolation and characterization of AgaSGNH, a cDNA encoding a member of the SGNH-hydrolase superfamily from young leaf epidermis of the monocot Agave americana L., is reported. The protein possesses a typical signal peptide at its N-terminus that allows its secretion to the epidermis cell wall, as verified by immunolocalization experiments. In addition, the AgaSGNH sequence contains a His-Leu-Gly-Ala-Glu (HLGAE) motif which is similar to that observed in other plant acyltransferases. Expression levels by northern blot and in situ localization of the corresponding mRNA, as well as the immunolocalization of the protein in Agave young leaves indicate that the protein is specifically present in the epidermal cells. The detailed study performed in different parts of the Agave leaf confirms two aspects: first, the expression of AgaSGNH is limited to the epidermis, and second, the maximum mRNA levels are found in the epidermis of the youngest zones of the leaf which are especially active in cutin biosynthesis. These levels dramatically decrease in the oldest zone of the leaf, where the presence of AgaSGNH mRNA is undetectable, and the biosynthesis of different cuticle components is severely reduced. These data could be compatible with the hypothesis that AgaSGNH could carry out both the hydrolysis and the transfer, from an activated acyl-CoA to a crescent cutin in Agave americana leaves and, therefore, be involved in the still unknown mechanism of plant cutin biosynthesis.

  14. INFORME GENERAL DE LA 56ava REUNION ANUAL DE LA SOCIEDAD AMERICANA DE ENDODONCIA REALIZADA EN LA CIUDAD DE ATLANTA-GEORGIA ENTRE EL 21 Y EL 24 DE ABRIL DE 1999.

    Chavez de Paz Villanueva, Luis Esuardo

    2014-01-01

    El programa de la quicuagésima sexta reunión anual de la Sociedad Americana de Endodoncia fue, como todos los años,el evento académico mas importante encuanto a endodoncia se refiere y a la cual tuve el honor de asistir por segundo año consecutivo. El programa de la quicuagésima sexta reunión anual de la Sociedad Americana de Endodoncia fue, como todos los años,el evento académico mas importante encuanto a endodoncia se refiere y a la cual tuve el honor de asistir por segundo año consecuti...

  15. As famílias na abordagem Minskyana: aspectos e desdobramentos do endividamento das famílias americanas no século XX e início do XXI

    EVERTON S. T. ROSA

    2016-03-01

    Full Text Available RESUMO Este artigo busca retratar a inserção financeira das famílias e suas implicações para a dinâmica do sistema econômico, tendo em vista a importância assumida pelo endividamento das famílias norte-americanas nas últimas décadas, em particular, na crise de 2007 nos EUA. Frente às transformações da economia norte-americana e de seu sistema financeiro, é proposto o resgate das contribuições da economia monetária de Keynes e da visão financeira de Minsky para compreender de forma adequada o comportamento das famílias, tanto na dimensão dos fluxos de renda e gastos, quanto nas decisões sobre estoques de ativos e passivos sujeitos à volatilidade e incerteza.

  16. Aspectos epidemiológicos da Leishmaniose Tegumentar Americana no município de Juína, Mato Grosso, Brasil = Epidemiological aspects of American Cutaneous Leishmaniasis in the city of Juína, Mato Grosso state, Brazil

    França, Eduardo Luzia

    2009-01-01

    Conclusões: a análise dos dados revelou que a Leishmaniose Tegumentar Americana é uma parasitose importante no município de Juína. Os dados sugerem relação com a atividade laboral e um perfil de transmissão predominantemente silvestre, o que deve ser levado em conta ao definir medidas de controle da doença na região

  17. Synthèses enzymatiques de néoglucoconjugués catalysées par l'alpha-glucosidase purifiée de la blatte Periplaneta americana (Linnaeus

    Kamenan A.

    2005-01-01

    Full Text Available Enzymatic synthesis of neoglucoconjugates by purified α-glucosidase from cockroach Periplaneta americana (Linnaeus. Cockroach Periplaneta americana (Linnaeus contains in his digestive tract an acid (pH 5,0 and mesophile (50°C α-glucosidase. This enzyme, purified to homogeneity, hydrolyses highly maltose, sucrose and p-nitrophenyl-α-Dglucopyranoside. The ability of α-glucosidase from cockroach purified to homogeneity to catalyse transglucosylation reactions was tested using maltose and saccharose as glucosyl donors and 2-phenylethanol and phenol as acceptors. The experimental conditions were optimized in relation to the time course of the reaction, pH and concentrations of glucosyl donors and acceptors. The yields in transglucosylation reactions at 37 °C were very high and could attain 67% and 48% with 2-phenylethanol and phenol respectively as glucosyl acceptors. This α-glucosidase hydrolyzed the products formed. It seems that the products formed were the phenylethyl-α-D-glucoside and phenyl-α-D-glucoside. These results suggest that α- glucosidase from cockroach is an exoglucosidase which catalyse the splitting of the α-glucosyl residue from the non reducing terminal of the substrate to liberate α-glucose. This comportment indicates that this enzyme operated by a mechanism involving the retention of the anomeric configuration. On the basis of this work, α-glucosidase from P. americana appears to be a valuable tool for the preparation of α-neoglucoconjugates.

  18. Effects of Persea americana Mill (Lauraceae) ["Avocado"] ethanolic leaf extract on blood glucose and kidney function in streptozotocin-induced diabetic rats and on kidney cell lines of the proximal (LLCPK1) and distal tubules (MDBK).

    Gondwe, M; Kamadyaapa, D R; Tufts, M A; Chuturgoon, A A; Ojewole, J A O; Musabayane, C T

    2008-01-01

    Extracts of Persea americana Mill (Lauraceae) ("Avocado") have been traditionally used to treat hypertension and diabetes mellitus. Accordingly, we studied the hypoglycaemic and renal function effects of P. americana leaf ethanolic extracts (PAE) in STZ-induced diabetic rats. Oral glucose tolerance responses to various doses of PAE were monitored in fasted rats following a glucose load. Rats treated with deionized water or standard hypoglycaemic drugs acted as untreated and treated positive controls, respectively. Acute renal effects of PAE were investigated in anesthetized rats challenged with 0.077 M NaCl after a 3.5-h equilibration for 4 h comprising 1 h control, 1.5 h treatment and 1.5 h recovery periods. PAE was added to the infusate during the treatment period. Hepatic glycogen concentration was measured after 6 weeks of daily treatment with PAE. PAE induced dose-dependent hypoglycaemic responses in STZ-induced diabetic rats while subchronic PAE treatment additionally increased hepatic glycogen concentrations. Acute PAE infusion decreased urine flow and electrolyte excretion rates, whilst subchronic treatment reduced plasma creatinine and urea concentrations. These results indicate not only the basis of the ethnomedicinal use of P. americana leaf extract in diabetes management, but also of need for further studies to identify and evaluate the safety of PAE's bioactive compounds.

  19. Externalidades e mudanças da regulamentação ambiental para a suinocultura norte-americana: é possível no caso brasileiro?

    Celso Leonardo Weydmann

    2005-06-01

    Full Text Available Neste trabalho objetiva-se descrever a relação entre externalidade e regulação ambiental com base no desenvolvimento recente da suinocultura norte-americana para avaliar a possibilidade de uma reforma na legislação que permita um controle ambiental mais rigoroso dos dejetos da suinocultura brasileira. A análise evidenciou que se a reforma fosse feita aos moldes da norte-americana teria que ser uma iniciativa do governo federal para definir metas nacionais de padrões polutivos com base na consolidação de regras estaduais. Recursos públicos seriam necessários para fiscalizar e punir poluidores, e para financiar os produtores na aquisição dos insumos ambientais. Por outro lado, as pressões da sociedade estariam restritas à atuação de organizações isoladas de pesquisa e extensão. A conclusão é de que no curto-prazo não há condições econômicas, sociais e institucionais que apontem para a realização de reforma.This paper describes the relationship between externalities and environmental regulation in the USA hog sector to verify the possibility to increase the stringency of regulation concerning externalities caused by hog manure in Brazil. The analysis shows that if the regulation reform in Brazil is going to follow the one in the USA it should be a government iniciative through the definition of national polluting standards based on hog manure rules already in place by the Brazilian states. Public funds would be required to enforce and punish polluters, and also to support farmers to acquire environmental inputs. On the other side, social pressure would be confined from the action of isolated groups related to environment, agriculture and farmer's work extension. The main conclusion is that there are no economic, social and institutional factors indicating the feasibility of such reform in the short run.

  20. Nonhost status of commercial Persea americana 'Hass' to Anastrepha ludens, Anastrepha obliqua, Anastrepha serpentina, and Anastrepha striata (Diptera: Tephritidae) in Mexico.

    Aluja, Martín; Díaz-Fleischer, Francisco; Arredondo, José

    2004-04-01

    The objective of this study was to determine the host status in Mexico of commercially cultivated and marketed avocado, Persea americana (Mill.), 'Hass' to Anastrepha ludens (Loew), Anastrepha obliqua (Macquart), Anastrepha serpentina (Wiedemann), and Anastrepha striata (Schiner) (Diptera: Tephritidae). Experiments in Michoacán, Mexico, were carried out in six orchards located at three altitudes above sea level during two times (August-October 2001 and April-June 2002). They included choice ('Hass' avocado plus natural host) and no-choice foraging behavior tests on trees under field cages; no-choice, forced infestation trials on caged, fruit-bearing branches in the field, and with individual fruit under laboratory conditions; infestation trials using 'Hass' avocados left unprotected over 1 and 7 d on the ground of orchards; studies to ascertain depth of oviposition and determine egg hatchability; and experiments to determine susceptibility by using time elapsed since removal of fruit from tree as the experimental variable. We trapped adult Anastrepha (n = 7,936) in all orchards and dissected fruit (n = 7,695) from orchards and packing houses (n = 1,620) in search of eggs or larvae. Most (96.7%) A. ludens, A. obliqua, A. striata, and A. serpentina adults were captured in low-elevation orchards. No eggs or larvae were detected in any of the fruit from foraging behavior studies or dissected fruit from orchards or packing houses. Of 5,200 mature, intact fruit on trees in the field forcibly exposed to no-choice female oviposition activity (five females/fruit), we only found four fruit infested by A. ludens but no adults emerged. 'Hass' avocados only became marginally susceptible to attack by A. ludens (but not A. obliqua, A. serpentina, and A. striata) 24 h after being removed from the tree. Fruit placed on the ground in orchards (n = 3,600) were occasionally infested by Neosilba batesi (Curran) (Diptera: Lonchaeidae), a decomposer, but not Anastrepha spp. Based on our

  1. A literatura latino-americana e as alegorias nacionais de Concierto Barroco = Latin-American Literature and national allegories present in the “novella” Concierto Barroco

    Maryllu Oliveira Caixeta

    2011-01-01

    Full Text Available A identidade problemática da América Latina reflete-se na recorrência do tema na literatura realizada nessa parte do continente. As imagens de Caliban, do antropófago e a atribuição de um sentido barroco à experiência latino americana caracterizam uma parte expressiva da literatura em questão. O Concierto Barroco de Carpentier exemplifica, por meio do personagem Amo, as dificuldades da elite crioula em encontrar uma origem nobre para si na Europa de seus avós e mesmo de identificar-se com ela. O personagem escravo músico Filomeno naturaliza a fabulação, assim como o Amo, e transita abusadamente entre as hierarquias, alegorizando o sentido barroco da identidade em questão. O jazz é o produto crioulo, antropofágico e barroco de negros cuja origem apagada na história foi substituída pela fabulação nos spirituals, num caminho musical que renovou a música moderna nomundo todo. É significativo que Carpentier tenha criado por meio de Filomeno a unidade da experiência americana, cuja fronteira é o México. The problematical identity of Latin America is reflected on the recurrence of the theme in the literature created in this part of the continent. The images of Caliban and anthropophagy and the attribution of a Baroque meaning to the Latin American experience characterize an expressive part of such literature. By means of thecharacter Amo, Carpentier's Concierto Barroco exemplifies the difficulties of the Creole elite in finding a noble origin for themselves in their ancestors' Europe, and in identifying themselves with it. The slave musician Filomeno and Amo naturalizes narrative, whereas Filomeno provocatively transits between the hierarchies, turning the Baroque meaning of identity into an allegory. Jazz is a Creole, anthropophagic and Baroque product whose erased origin was replaced by the fabled narrative in the Spirituals that renewed modernmusic. It is important to emphasize that Carpentier created, by means of Filomeno

  2. DESAFIOS DA PRODUÇÃO AGRÍCOLA CAMPONESA NOS ASSENTAMENTOS DE REFORMA AGRÁRIA Assentamento Milton Santos – Americana/SP

    Larissa Mies Bombardi

    2009-12-01

    Full Text Available  Este artigo aborda a Experiência de Extensão em Geografia Agrária coordenada pelas Profas Dras Larissa Mies Bombardi e Sidneide Manfredini, e realizada em conjunto com um grupo de alunos do Depto. De Geografia da USP. Tem como finalidade colaborar com as práticas agrícolas e a comercialização que se iniciam no Assentamento Milton Santos localizado nos municípios de Americana e Cosmopólis – São Paulo. Este assentamento apresenta peculiaridades que se colocam como um grande desafio a sua efetiva implantação. Concebido nos moldes de Comuna da Terra, é constituído em sua quase totalidade por famílias de origem urbana, está localizado em uma região monocultora de cana-de-açúcar e dista cerca de 10km das áreas urbanas dos municípios aos quais está afeto (Americana, Cosmópolis e Paulínia. À medida que se conseguiu agregar competências diversas ao grupo inicialmente constituído exclusivamente por geógrafos, com alunos e professores da Escola Superior de Agricultura Luiz de Queiroz, da Engenharia Sanitária da Escola Politécnica e da Faculdade de Arquitetura da USP, o projeto foi se ampliando e redirecionando seus objetivos iniciais. A implementação de sistemas agrícolas otimizados, que condicionariam a efetiva implantação do assentamento, torna imprescindível o resgate da cultura camponesa, essencialmente nos elementos que concorrem para a apreensão e apropriação da paisagem, tanto quanto dos conhecimentos científicos embutidos nas tecnologias a serem empregadas, ou dos limites impostos pela legislação que regem o licenciamento ambiental dos projetos de reforma agrária. As ações deste grupo de trabalho passaram a se dar no sentido da intermediação na apropriação destes conhecimentos, numa perspectiva de gestão participativa que venha a concorrer para a estruturação da tessitura social deste coletivo, levando os assentados a se perceberem como sujeitos no processo de produção deste novo espaço

  3. Os mexicanos-americanos e a nação americana: resposta ao professor Huntington Mexican-Americans and the American nation: a response to professor Huntington

    Edward Telles

    2006-11-01

    Full Text Available Este artigo baseia-se em uma palestra dada na Texas A&M University em 10 de dezembro de 2005, em resposta a uma palestra anterior do professor Samuel P. Huntington. Os dados obtidos pela ciência social são usados para analisar a tese de Huntington de que os mexicanos estão sobrecarregando as fronteiras americanas. O artigo analisa ainda a evidência de que os mexicanos-americanos estão sendo assimilados culturalmente, mas, em termos econômicos, não são tão bem-sucedidos quanto os descendentes dos primeiros imigrantes europeus; e examina os fatores que diferenciam, e provavelmente continuarão a fazê-lo, a trajetória de incorporação dos mexicanos-americanos. Por fim, recomenda que a opinião pública americana e os políticos façam opções fundamentadas a respeito dos níveis desejáveis de imigração e das pessoas que devem ser aceitas, aperfeiçoem por meio da educação as oportunidades econômicas dos imigrantes e fomentem um futuro multilinguístico e multiétnico para o país.This essay is based on a talk I delivered at Texas A&M University on December 10, 2005, in response to an earlier lecture at the university by Professor Samuel P. Huntington. It relies on social science evidence to first address Huntington's contention that Mexicans are overwhelming American borders. It then turns to evidence that Mexican Americans are in fact assimilating culturally but still have been less economically successful than the descendants of earlier European immigrants. The essay examines factors that have differentiated the Mexican American trajectory of incorporation and are likely to continue to do so. Finally, it calls for the American public and policy makers to make well-informed choices about what levels of immigration are desirable and who should be admitted, to improve immigrants' economic opportunities through education, and to embrace a multilingual and multiethnic future for the country.

  4. Human-machine Inter-permeability in Americana%《美国万花筒》中的人机互渗现象

    刘岩

    2016-01-01

    评论界对唐·德里罗第一部小说《美国万花筒》的主人公大卫·贝尔的主体身份问题有两种截然相反的看法。文章从贝尔身份问题的二重性切入,结合学界有关人机/技关系问题的讨论,通过分析主人公大卫·贝尔与小说中占据重要位置的电视和摄影机等现代媒介机器的关系特征,认为贝尔的主体身份问题之所以引起两种对立的解读,是因为后现代文化环境下人机互动变得深刻复杂,并进而提出《美国万花筒》捕捉并呈现了人机关系发展的一个新阶段—人机互渗,理解和把握后现代文化环境下人机互渗现象是理解德里罗小说另类主体重建的前提。%There are double-readings about the subjectivity of David Bell the hero of Don DeLillo’s first novel Americana. It is held in this article that the double-readings result from the profound and complicated interplay between human and technology/machine against the backdrop of postmodern cultural environment. Based on the discussions about the essence of the relationship between human and machine, the article makes an analysis of such media machine as television and camera which function as more than mere material objects and concludes that DeLillo captures in Americana a kind of phenomenal human-machine inter-permeability, a new development of human-machine relationship. The new human-machine relationship is the premise on which the postmodern subjectivity is to be reconstructed against the postmodern society.

  5. Atividade antioxidante e quantiï¬ cação de compostos bioativos dos frutos de abricó (Mammea americana)
    Antioxidant activity and bioactive compounds of the abricó fruits (Mammea americana)

    A. C. C. BRAGA; Silva, A.E.; A. C. A. PELAIS; C. M. G. BICHARA; D. R. POMPEU

    2010-01-01

    O presente trabalho apresenta a atividade antioxidante, a quantifi cação de compostos bioativos (carotenóides totais – CT, polifenóis totais – PT e fl avanóis totais – FT), e a caracterização física e centesimal dos frutos de abricó (Mammea americana). Foram realizadas análises de cor (sistema L*, a* e b*), atividade antioxidante (Oxigen Radical Absorbance Capacity – ORAC – e Trolox Equivalent Antioxidant Capacity – TEAC), de CT (método espectr...

  6. Tratamiento contable de las transacciones separadas en las combinaciones de negocios. Proceso armonizador de la normativa contable internacional (IFRS y americana (FAS

    Montserrat Manzaneque Lizano

    2013-01-01

    Full Text Available Los cambios introducidos en la FAS 141(R (2007 americana y en la IFRS 3 (R (2008, con el fin de armonizar los aspectos contables de las operaciones referentes a la combinación de negocios, han puesto de manifiesto la existencia de determinadas transacciones que no forman parte de la propia combinación y a las que se denomina transacciones separadas, cuya delimitación de la combinación de negocios queda dispersa en ambas normas, por lo que en ocasiones resulta poco clarificador. Así, este trabajo pretende facilitar al lector la comprensión de su tratamiento contable abordando la problemática de identificar las transacciones que se producen, y las que no, como consecuencia de la combinación de negocios, el tratamiento contable de esta disyuntiva y su efecto sobre el reconocimiento y valoración del fondo de comercio (cuenta de crédito mercantil.

  7. Bases das escolas Européia Norte e Americana, perante a cultura contábil e a proposta neopatrimonialista

    Antônio Lopes de Sá

    2002-11-01

    Full Text Available Como entender a matéria contábil, o método que se emprega para estudá-la, o respeito às tradições históricas como conquistas já feitas, as grandes finalidades desse conhecimento, em suma, como possuir uma visão realista e verdadeira sobre os fenômenos da riqueza individualizada é o que caracteriza uma "escola científica de Contabilidade". Diversos desses núcleos se formaram e ainda se formam, não só no setor contábil, mas, em todas as ciências, sempre em torno de lideranças, quer de pensadores, quer de grupos interessados em manipular idéias. Algumas escolas, todavia, basearam-se apenas no consenso de grupos e se ataram a um raciocínio pragmático. Por serem diferentes as concepções e os efeitos do tratamento que se tem dado à Contabilidade, as principais distinções de entendimento encontram-se, na atualidade, entre as escolas norte-americanas, pragmáticas e as européias, científicas. No Brasil a corrente científica do neopatrimonialismo oferece novas propostas em suas bases, mas, mantendo a fidelidade com as raízes latinas do conhecimento contábil.

  8. Leishmaniose tegumentar americana: flebotomíneos em área de transmissão no Município de Uberlândia, MG

    Lemos Jureth Couto

    2005-01-01

    Full Text Available De maio de 2000 a janeiro de 2001, foram realizadas 4 capturas de flebotomíneos em área de transmissão da leishmaniose tegumentar americana na bacia do rio Araguari, no Município de Uberlândia, MG, com duração de 15 horas consecutivas cada, para observar o comportamento dos insetos. A primeira captura foi em maio de 2000 (frio e úmido, a segunda em junho de 2000 ( frio e seco, a terceira em outubro de 2000 (quente e seco e a quarta em janeiro de 2001 (quente e chuvoso. Utilizaram-se para capturar os flebótomos armadilhas Center on Disease Control e Shannon. Foram capturados e identificados 6551 flebótomos, sendo 1990 machos e 4561 fêmeas distribuídos em 2 gêneros (Lutzomyia e Brumptomyia e 8 espécies. A Lutzomyia intermedia predominou com o maior número de espécimens (6531, representando 99,7% dos flebótomos capturados. Nas quatro capturas observou-se a preferência de Lutzomyia intermedia pelo mês que precede o período chuvoso (outubro, com temperaturas e umidade relativa do ar altas.

  9. Apios americana Medik Extract Alleviates Lung Inflammation in Influenza Virus H1N1- and Endotoxin-Induced Acute Lung Injury.

    Sohn, Sung-Hwa; Lee, Sang-Yeon; Cui, Jun; Jang, Ho Hee; Kang, Tae-Hoon; Kim, Jong-Keun; Kim, In-Kyoung; Lee, Deuk-Ki; Choi, Seulgi; Yoon, Il-Sub; Chung, Ji-Woo; Nam, Jae-Hwan

    2015-12-28

    Apios americana Medik (hereinafter Apios) has been reported to treat diseases, including cancer, hypertension, obesity, and diabetes. The therapeutic effect of Apios is likely to be associated with its anti-inflammatory activity. This study was conducted to evaluate the protective effects of Apios in animal models of acute lung injury induced by lipopolysaccharide (LPS) or pandemic H1N1 2009 influenza A virus (H1N1). Mice were exposed to LPS or H1N1 for 2-4 days to induce acute lung injury. The treatment groups were administered Apios extracts via oral injection for 8 weeks before LPS treatment or H1N1 infection. To investigate the effects of Apios, we assessed the mice for in vivo effects of Apios on immune cell infiltration and the level of pro-inflammatory cytokines in the bronchoalveolar lavage (BAL) fluid, and histopathological changes in the lung. After induction of acute lung injury, the numbers of neutrophils and total cells were lower in the Apios-treated groups than in the non-Apios-treated LPS and H1N1 groups. The Apios groups tended to have lower levels of tumor necrosis factor-a and interleukin-6 in BAL fluid. In addition, the histopathological changes in the lungs were markedly reduced in the Apios-treated groups. These data suggest that Apios treatment reduces LPS- and H1N1-induced lung inflammation. These protective effects of Apios suggest that it may have therapeutic potential in acute lung injury.

  10. Distribuição geográfica da leishmaniose tegumentar americana no Estado do Espírito Santo - Brasil

    Paulo Augusto Sessa

    1985-12-01

    Full Text Available Com base nos registros de 730 pacientes portadores de Leishmaniose Tegumentar Americana (LTA referentes ao período de janeiro de 1972 a dezembro de 1982, os autores realizaram um estudo da distribuição geográfica dessa parasitose no Estado do Espírito Santo. Os pacientes procediam de 36 dos 53 municípios que compõem o Estado, destacando-se Viana e Cariacica como os de maior prevalência, somando juntos 442 casos (60,54%, com 259 destes residindo numas poucas localidades, contínuas entre si, formando uma área endêmica, com transmissão ocorrendo provavelmente no peri e intra-domicílio. Esta área de alta endemicidade, pertencente principalmente ao vale do rio Formate, estende-se também ao município de Domingos Martins através da localidade de Biriricas. Nos demais municípios a LTA caracterizou- se como uma doença profissional de ocorrência antiga no Estado.

  11. Etnogerontología. La posición de la Sociedad Americana de Geriatría sobre etnogeriatría.

    Herman F. Curiel

    2009-10-01

    Full Text Available Las minorías de ancianos en los Estados Unidos tendrán un crecimiento acelerado en las próximas décadas. Incluyen ancianos de grupos étnicos heterogéneos como negros, hispanos, asiáticos e indígenas americanos. El Comité de Etnogeriatría de la Sociedad Americana de Geriatría, SAG, trabaja en aspectos relacionados con la salud y el bienestar de tales minorías. Se informa la Declaración de Posición en Etnogeriatría de la SAG. La SAG promueve la sensibilidad multicultural, la educación y la investigación interdisciplinaria sobre los factores étnicos que afectan el envejecimiento, la salud y la aparición de enfermedades en las personas mayores. Las minorías de ancianos tienen una alta prevalencia de cuadros clínicos patológicos en edades tempranas que afectan la funcionalidad y calidad de vida de estas personas. También hay barreras lingüísticas o culturales, pobreza, y bajo nivel educativo que disminuyen el acceso al cuidado de la salud. La SAG apoya conocer y superar esos factores que influyen sobre la salud de las minorías de ancianos en América.

  12. A new species of Syncuaria Gilbert, 1927 (Nematoda: Acuarioidea: Acuariidae) in the wood stork, Mycteria americana L. (Aves: Ciconiiformes: Ciconiidae) from the Area de Conservacion Guanacaste, Costa Rica.

    Zhang, Luping; Brooks, Daniel R; Causey, Douglas

    2003-10-01

    Syncuaria mycteriae n. sp. (Nematoda: Acuarioidea) was collected under the lining of the gizzard of a wood stork, Mycteria americana L., from the Area de Conservacion Guanacaste, Costa Rica. The new species can be distinguished from all known species of Syncuaria by having irregular dotted ornamentations on the caudal alae of males, a complex distal end of the left spicule comprising 3 protuberances, and a spicule ratio of 1:9.3. Preliminary phylogenetic analysis of 11 Syncuaria spp. based on 9 morphological characters produced 2 equally parsimonious cladograms with a consistency index of 85%, differing only in the placement of S. hargilae. The phylogenetic analysis suggests that the new species is the sister species of S. leptoptili, whose male members have a single protuberance on the left spicule. Furthermore, the analysis suggests that the plesiomorphic host group for the genus is Ciconiiformes, specifically Ciconiidae (host for 5 species), with 2 species occurring in Threskiornithidae (also Ciconiiformes), possibly as a result of cospeciation, and 2 species each occurring in Pelecaniformes and Podicipediformes, resulting from 4 episodes of speciation by host switching.

  13. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus

    Jaquelina Julia Guzmán-Rodríguez

    2013-01-01

    Full Text Available Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP represent attractive control agents. Mexican avocado (Persea americana var. drymifolia is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp encoding a protein (78 aa homologous with plant defensins (>80%. We expressed the defensin PaDef cDNA (pBME3 in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%. Also, S. aureus viability was inhibited from 50 μg/mL total protein (27–38% but was more evident at 100 μg/mL (52–65%. This inhibition was higher than the effect showed by polyclonal population (~23%. Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

  14. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Suárez-Rodríguez, Luis M.; Salgado-Garciglia, Rafael; Rodríguez-Zapata, Luis C.; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E.

    2013-01-01

    Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 μg/mL total protein (27–38%) but was more evident at 100 μg/mL (52–65%). This inhibition was higher than the effect showed by polyclonal population (~23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens. PMID:24319695

  15. Vigilância de leishmaniose visceral americana em cães de área não endêmica, São Paulo

    Savani Elisa San Martin Mouriz

    2003-01-01

    Full Text Available Realizou-se inquérito sorológico em cães domiciliados e errantes do Município de São José do Rio Preto, SP, para identificar animais infectados e detectar a possibilidade de transmissão da leishmaniose visceral americana. De novembro de 1998 a junho de 2000, 2.104 amostras de soros foram testadas por meio da reação de imunofluorescência indireta, empregando-se como antígeno formas promastigotas de Leishmania (L. chagasi. Observaram-se 2.092 amostras não reagentes e 12 reagentes. Dos cães com sorologia positiva foi possível realizar raspados de lesão em três animais. O material fixado em lâminas foi corado por Giemsa e, em apenas um, foram encontradas formas amastigotas características de Leishmania sp. Este resultado indica a necessidade de manutenção da vigilância sorológica canina e entomológica no município de São José do Rio Preto, a fim de detectar, precocemente, qualquer alteração na epidemiologia local.

  16. Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: I. investigação epidemiolôgica clínica e laboratorial

    Nelson A. Araújo Filho

    1981-12-01

    Full Text Available Os autores descrevem pela primeira vez a ocorrência de uma epidemia de Leishmaniose Tegumentar Americana (LTAI na localidade de Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, situada a 17 Km do continente. Em uma população de 453 pessoas examinadas, foram encontrados 33 casos de LTA, sendo 13 lesões em atividade e 20 cicatrizes. Entre os 33 casos, 30 eram autóctones da área de estudo. A doença ocorria há mais de 10 anos na Praia Vermelha, e, na Ilha Grande, há mais de 30 anos, sob a forma de casos esporádicos. Na Praia Vermelha, no período de 1974 a 1976, ocorreu uma epidemia com 27 casos da doença. A L TA, na Praia Vermelha, assume características de transmissão em ambiente domiciliar, atingindo indivíduos indistintamente, sem relação com sexo, idade ou profissão. Predominou a forma ulcerada, em extremidades do corpo com ocorrência de cura espontânea das lesões e não comprometimento das mucosas; um hamster entre 10 inoculados com material de lesão de caso humano desenvolveu, após 4 meses, lesão de focinho com resultados positivos para LTA.

  17. Thermal stability of oils added with avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) leaf extracts during the French potatoes frying.

    Jiménez, Paula; García, Paula; Bustamante, Andrés; Barriga, Andrés; Robert, Paz

    2017-04-15

    Effect of the addition of avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) hydroalcoholic leaf extracts (AHE and OHE, respectively) on thermal stability of canola oil (CO) and high oleic sunflower oil (HOSO) during French potatoes frying at 180°C was studied. The extracts were characterized by the total phenolic content, phenol chromatographic profiles and antioxidant activity. B-type trimer procyanidins were the major phenolic compounds identified in AHE. OHE showed higher phenol content, antioxidant activity regarding AHE. CO+OHE and HOSO+OHE decreased the formation of polar compounds and showed an anti-polymeric effect with respect to oils without extracts, whereas AHE extract showed a prooxidant effect on HOSO. Therefore, OHE showed an antioxidant effect on HOSO and CO under the studied conditions. In addition, all systems (CO+AHE, HOSO+AHE, CO+OHE and HOSO+OHE) increased the retention of tocopherols. These results demonstrate the potential utility of OHE as natural antioxidant for oils.

  18. Stem Bark Extract and Fraction of Persea americana (Mill. Exhibits Bactericidal Activities against Strains of Bacillus cereus Associated with Food Poisoning

    David A. Akinpelu

    2014-12-01

    Full Text Available The study investigates the in vitro antibacterial potentials of stem bark extracts of Persea americana on strains of Bacillus cereus implicated in food poisoning. The crude stem bark extracts and butanolic fraction at a concentration of 25 mg/mL and 10 mg/mL, respectively, exhibited antibacterial activities against test isolates. The zones of inhibition exhibited by the crude extract and the fraction ranged between 10 mm and 26 mm, while the minimum inhibitory concentration values ranged between 0.78 and 5.00 mg/mL. The minimum bactericidal concentrations ranged between 3.12 mg/mL–12.5 mg/mL and 1.25–10 mg/mL for the extract and the fraction, respectively. The butanolic fraction killed 91.49% of the test isolates at a concentration of 2× MIC after 60 min of contact time, while a 100% killing was achieved after the test bacterial cells were exposed to the butanolic fraction at a concentration of 3× MIC after 90 min contact time. Intracellular protein and potassium ion leaked out of the test bacterial cells when exposed to certain concentrations of the fraction; this is an indication of bacterial cell wall disruptions by the extract’s butanolic fraction and, thus, caused a biocidal effect on the cells, as evident in the killing rate test results.

  19. Avocado (Persea americana Mill.) phenolics, in vitro antioxidant and antimicrobial activities, and inhibition of lipid and protein oxidation in porcine patties.

    Rodríguez-Carpena, Javier-Germán; Morcuende, David; Andrade, María-Jesús; Kylli, Petri; Estévez, Mario

    2011-05-25

    The first aim of the present work (study 1) was to analyze ethyl acetate, 70% acetone, and 70% methanol extracts of the peel, pulp, and seed from two avocado (Persea americana Mill.) varieties, namely, 'Hass' and 'Fuerte', for their phenolic composition and their in vitro antioxidant activity using the CUPRAC, DPPH, and ABTS assays. Their antimicrobial potential was also studied. Peels and seeds had higher amounts of phenolics and a more intense in vitro antioxidant potential than the pulp. Peels and seeds were rich in catechins, procyanidins, and hydroxycinnamic acids, whereas the pulp was particularly rich in hydroxybenzoic and hydroxycinnamic acids and procyanidins. The total phenolic content and antioxidant potential of avocado phenolics was affected by the extracting solvent and avocado variety. The avocado materials also displayed moderate antimicrobial effects against Gram-positive bacteria. Taking a step forward (study 2), extracts (70% acetone) from avocado peels and seeds were tested as inhibitors of oxidative reactions in meat patties. Avocado extracts protected meat lipids and proteins against oxidation with the effect on lipids being dependent on the avocado variety.

  20. Determination of changes in the metabolic profile of avocado fruits (Persea americana) by two CE-MS approaches (targeted and non-targeted).

    Contreras-Gutiérrez, Paulina K; Hurtado-Fernández, Elena; Gómez-Romero, María; Ignacio Hormaza, José; Carrasco-Pancorbo, Alegría; Fernández-Gutiérrez, Alberto

    2013-10-01

    A CZE method with two different MS detection conditions (MRM and Full Scan) was developed to determine qualitative and quantitative changes in the metabolic profile of avocado fruits (Persea americana). LODs in MRM approach were found between 20.1 and 203.0 ppb for abscisic acid and perseitol, respectively, whilst in Full Scan, varied within the range 0.22–1.90 ppm for the same metabolites. The RSDs for reproducibility test did not exceed 11.45%. The two MS approaches were used to quantify 10 metabolites (phenolic acids, flavonoids, a carbohydrate, an organic acid, a vitamin and a phytohormone) in 18 samples of avocado at different ripening states, and the achieved results were compared. Perseitol, quinic, chlorogenic, trans-cinnamic, pantothenic and abscisic acids, as well as epicatechin and catechin decreased during the ripening process, whereas ferulic and p-coumaric acids showed the opposite trend. Moreover, some other unknown compounds whose concentration changed largely during ripening were also studied by MS/MS and QTOF MS to get a tentative identification.

  1. The defensin from avocado (Persea americana var. drymifolia) PaDef induces apoptosis in the human breast cancer cell line MCF-7.

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Salgado-Garciglia, Rafael; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2016-08-01

    Antimicrobial peptides (AMPs) are cytotoxic to cancer cells; however, mainly the effects of AMPs from animals have been evaluated. In this work, we assessed the cytotoxicity of PaDef defensin from avocado (Persea americana var. drymifolia) on the MCF-7 cancer cell line (a breast cancer cell line) and evaluated its mechanism of action. PaDef inhibited the viability of MCF-7 cells in a concentration-dependent manner, with an IC50=141.62μg/ml. The viability of normal peripheral blood mononuclear cells was unaffected by this AMP. Additionally, PaDef induced apoptosis in MCF-7 cells in a time-dependent manner, but did not affect the membrane potential or calcium flow. In addition, PaDef IC50 induced the expression of cytochrome c, Apaf-1, and the caspase 7 and 9 genes. Likewise, this defensin induced the loss of mitochondrial Δψm and increased the phosphorylation of MAPK p38, which may lead to MCF-7 apoptosis by the intrinsic pathway. This is the first report of an avocado defensin inducing intrinsic apoptosis in cancer cells, which suggests that it could be a potential therapeutic molecule in the treatment of cancer.

  2. Biochemical analysis of reactive oxygen species production and antioxidative responses in unripe avocado (Persea americana Mill var Hass) fruits in response to wounding.

    Castro-Mercado, E; Martinez-Diaz, Y; Roman-Tehandon, N; Garcia-Pineda, E

    2009-03-01

    We analyzed the production of reactive oxygen species (ROS) and of detoxifying enzymes and enzymes of the ascorbate (ASC) acid cycle in avocado fruit (Pesea Americana Mill cv Hass) in response to wounding. The levels of superoxide anion (O(2-), hydroxyl radicals (OH.) and hydrogen peroxide (H(2)O(2)) increased at 15 min and 2 and 15 h post-wounding. Peroxidase (POD) activity had increased to high levels 24 h after wounding; in contrast, catalase and superoxide dismutase (SOD) levels hat decreased significantly at 24 h post-treatment. Basic POD was the major POD form induced, and the levels of at least three apoplastic POD isozymes -increased following wounding. Using specific inhibitors, we characterized one MnSOD and two CuZnSOD isozymes. CuZnSOD activities decreased notably 12 h after treatment. The activities of dehydroascorbate reductase and glutathione reductase increased dramatically following the wounding treatment, possibly as a means to compensate for the redox changes due to ROS production.

  3. Stem bark extract and fraction of Persea americana (Mill.) exhibits bactericidal activities against strains of bacillus cereus associated with food poisoning.

    Akinpelu, David A; Aiyegoro, Olayinka A; Akinpelu, Oluseun F; Okoh, Anthony I

    2014-12-30

    The study investigates the in vitro antibacterial potentials of stem bark extracts of Persea americana on strains of Bacillus cereus implicated in food poisoning. The crude stem bark extracts and butanolic fraction at a concentration of 25 mg/mL and 10 mg/mL, respectively, exhibited antibacterial activities against test isolates. The zones of inhibition exhibited by the crude extract and the fraction ranged between 10 mm and 26 mm, while the minimum inhibitory concentration values ranged between 0.78 and 5.00 mg/mL. The minimum bactericidal concentrations ranged between 3.12 mg/mL-12.5 mg/mL and 1.25-10 mg/mL for the extract and the fraction, respectively. The butanolic fraction killed 91.49% of the test isolates at a concentration of 2× MIC after 60 min of contact time, while a 100% killing was achieved after the test bacterial cells were exposed to the butanolic fraction at a concentration of 3× MIC after 90 min contact time. Intracellular protein and potassium ion leaked out of the test bacterial cells when exposed to certain concentrations of the fraction; this is an indication of bacterial cell wall disruptions by the extract's butanolic fraction and, thus, caused a biocidal effect on the cells, as evident in the killing rate test results.

  4. Effect of different extraction methods on fatty acids, volatile compounds, and physical and chemical properties of avocado (Persea americana Mill.) oil.

    Moreno, Alicia Ortiz; Dorantes, Lidia; Galíndez, Juvencio; Guzmán, Rosa I

    2003-04-09

    Because Mexico is the number one producer of avocados in the world, this fruit has potential as a source for oil extraction. It is appropriate to further investigate the detailed changes that the oil undergoes when different extraction methods are applied. This research paper presents the study of the physical and chemical changes, the fatty acids profile, the trans fatty acid content, and the identification of volatile compounds of the oils from avocado pulp (Persea americana Mill.), obtained by four different extraction methods. The method with the greatest extraction yield was the combined microwave-hexane method. The amount of trans fatty acids produced in the microwave-squeezing treatment was <0.5 g/100 g. On the other hand, the amounts of trans fatty acids produced with the hexane and acetone treatments were 0.52 and 0.87 g/100 g, respectively. The method that caused the slightest modification to the oil quality was a novel combined extraction method of microwave-squeezing proposed by the authors.

  5. Antibacterial activity of defensin PaDef from avocado fruit (Persea americana var. drymifolia) expressed in endothelial cells against Escherichia coli and Staphylococcus aureus.

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Suárez-Rodríguez, Luis M; Salgado-Garciglia, Rafael; Rodríguez-Zapata, Luis C; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2013-01-01

    Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 μg/mL total protein (27-38%) but was more evident at 100 μg/mL (52-65%). This inhibition was higher than the effect showed by polyclonal population (~23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

  6. Enterobacteriáceas en partes externas del estadío adulto de Periplaneta americana “cucaracha” capturadas en el mercado Modelo, Iquitos, Perú

    Xandrix Augusto Ruiz Cabezas

    2015-06-01

    Full Text Available Este estudio, determinó la presencia de Enterobacteriáceas en partes externas del estadío adulto de Periplaneta americana “cucaracha” capturadas en el mercado Modelo de la ciudad de Iquitos. Fueron colectados un total de 68 “cucarachas”, y luego cada una fue depositada en un frasco estéril y trasladadas vivas para el análisis respectivo al laboratorio de Investigación de la Facultad de Ciencias Biológicas; después del aislamiento, las colonias observadas fueron sometidas a pruebas de identificación bioquímica tales como: Prueba de oxidasa, utilización de glucosa, lactosa y sacarosa (agar TSI, pruebas VP-RM, indol, hidrólisis de la úrea. La prevalencia de Enterobacteriáceas se determinó utilizando el método estadístico de distribución de frecuencias y la prueba no paramétrica de Chi cuadrado para prueba de hipótesis. De las 68 cucarachas analizadas se aislaron 118 colonias caracterizadas como lactosa positiva y lactosa negativa, representando el 71 % y 29 % respectivamente de las muestras analizadas.  Finalmente, se aisló e identificó 5 cepas bacterianas, categorizadas en 5 géneros: Providencia (25%, Edwardsiella (9%, Citrobacter (14%, Enterobacter (31% y Klebsiella (21%.

  7. Detecção sorológica e microbiológica de Salmonella spp. em emas (Rhea americana

    R.A. Pereira

    2012-08-01

    Full Text Available The fast agglutination screening test, using S. Typhimurium as the antigen, was compared with the standard bacterial method to identify rheas (Rhea americana contaminated with Salmonella spp.at slaughter.Seventy birds were serologically tested for Salmonella enterica Pullorum using a commercial antigen.Of these, 66 were also submitted to a macroagglutination test, using a strain of Salmonella Typhimurium isolated from rheas.All birds did not react with the commercial S. Pullorum antigen, but 37 were positive for the FAST-ST.The isolation of Salmonella spp. was verified in 66 (94.2% birds. 85.7% were found in liver samples, 60% in feces and 42.3% in cloacal swabs.A total of 16.6% were identified as being S. enterica enterica rugosa, 35.9% as S. Typhimurium, 46.5% as S.Newport and 0.9% as S. Anatum. An insignificant concordance between the results of bacterial isolation and the serological response was observed (k=0.016.The detection of Salmonella spp. by bacteriological and serological methods in samples from rheas must be deemed important, because birds without a clinical manifestation can be significant sources of salmonellas in food infections.

  8. Informação e conhecimento: análise da rede apl têxtil de americana/sp-Brasil

    Cibele Roberta Sugahara

    2012-08-01

    Full Text Available This paper reports on the analysis knowledge building process in the context of networked companies, more specifically in the American Textile Network APL/SP-Brazil (Local Productive Arrangement. To do so, a case study was used to analyze the information exchange for the development of joint activities in the Textile Network APL that incorporates several links of the textile supply chain (Spinning, Processing, Weaving and Tailoring, located in the surrounding of Americana,Sao Paulo. Based on earlier studies regarding the process of knowledge construction developed by Nonaka and Takeuchi (1997, a questionnaire with 7 (seven questions was applied to the experts making up the APL Textile network. The study involved 37 participant companies out of 51. From this instrument, it was possible to collect data to support the research regarding information exchange and knowledge sharing for the development of joint activities among the case study members. The work highlights the importance of sharing tacit knowledge that allows reconstructing and exploiting knowledge more broadly. That is why the externalization of tacit knowledge into explicit knowledge is also required in a networked environment.

  9. Genetic status of the wood stork (Mycteria americana) from the southeastern United States and the Brazilian Pantanal as revealed by mitochondrial DNA analysis.

    Lopes, I F; Tomasulo-Seccomandi, A M; Bryan, A L; Brisbin, I L; Glenn, T C; Del Lama, S N

    2011-08-30

    The wood stork (Mycteria americana) is a colonial wading bird that inhabits the Neotropical region from the southeastern United States (US) to northern Argentina. The species is considered to be endangered in the US due to degradation of its foraging and breeding habitat. In other parts of its range, such as in the Brazilian Pantanal region, breeding populations of this species appear to be stable. We compared the levels of genetic variability and population structuring of the US and the Pantanal breeding populations using mitochondrial DNA (mtDNA) control region sequences. Twenty-seven haplotypes were identified among 88 wood stork samples collected from eight breeding colonies in the US and eight in the Pantanal. Patterns indicative of heteroplasmy were observed in 35.3% of the mtDNA sequences that were examined. Significantly higher levels of haplotype diversity were observed in the Pantanal samples compared to those from the US, suggesting that during the last century, demographic declines or a recent evolutionary bottleneck reduced the levels of mtDNA variability of the US population. Analyses of genetic structuring revealed non-significant genetic differentiation between these regions, indicating that either the populations were only recently separated or that gene flow continues to occur at low levels. Haplotype network analysis indicated low current levels of gene flow between populations that were closely related in the past.

  10. El Observador español en Londres, un periódico fernandino contra la emancipación Americana

    Hernández González, Manuel

    1999-08-01

    Full Text Available This article studies the apparition, contents and repercussion of El Observador Español in London, a pro-absolutist review published in the capital city of Great Britain in response to the support given by the public opinion to the American emancipation. His director was Miguel Cabral de Morona, who had been critical of the Spanish conquest and a radical liberal in the Cádiz of the Cortes. This project was doomed to fail, but it was contested by important writers favourable to the American independence.

    Este artículo estudia la creación, difusión, contenidos y repercusión del Observador español en Londres, un periódico fernandino editado en la capital británica con el objetivo de contrarrestar la opinión pública favorable a la emancipación americana. Redactado por el madeirense Miguel Cabral de Noroña, antiguo crítico de la colonización española y liberal radical en el Cádiz de las Cortes, su proyecto estaba condenado de antemano al fracaso, pero mereció las réplicas de significados voceros de la causa emancipadora.

  11. An HPLC-ELSD Method for the Determination of Triterpenes in Sorbus decora and Sorbus americana Bark Used by the Eeyou Istchee Cree First Nation.

    Saleem, Ammar; Liu, Rui; Guerrero-Analco, José A; Bailie, Anna; Foster, Brian; Cuerrier, Alain; Johns, Timothy; Haddad, Pierre S; Arnason, John Thor

    2016-09-01

    Sorbus decora and Sorbus americana are used traditionally as medicine by the Eeyou Istchee Cree First Nation of the James Bay region of Quebec, Canada. Because the ethanol extracts of the bark and the isolated terpenes of these plants have shown promising in vivo antidiabetic effects, an analytical method was developed and validated by RP-HPLC-ELSD for the identification and quantification of eight lupane- and ursane-type terpenes. The extraction method reproducibly recovered the compounds above 70 % and the chromatographic separation of betulin, 23-hydroxy-betulin, 23,28-dihydroxylupan-20(29)-ene-3β-caffeate, betulinic acid, α-amyrin, uvaol, 3β,23,28-trihydroxy-12-ursene, and 23,28-dihydroxyursan-12-ene-3β-caffeate was achieved within 27 min by linear gradient. The method produced highly reproducible quantitative data at interday and intraday levels. The limits of detection were in the ng level on-column with remarkable range and linearity. The target compounds were present at mg levels in the populations, collected from inland (Mistissini and Nemaska) and costal (Waskagnish and Chisasibi) Cree communities of northern Quebec. A triterpene, 23-hydroxybetulin, was the most abundant, while betulinic acid and uvaol were minor constituents. Overall, HPLC-ELSD analyses produced very similar profiles and contents of the eight compounds in the plants collected from four geographic locations. The developed HPLC-ELSD method can be used as a targeted analysis of triterpenes in these medicinal plants.

  12. Inovação tecnológica e cargas de trabalho dos profissionais de saúde: revisão da literatura latino-americana

    Denise Elvira Pires de Pires

    2010-06-01

    Full Text Available O intenso processo de inovação tecnológica, na atualidade, provoca modificações no mundo do trabalho que podem afetar as cargas de trabalho e a saúde dos trabalhadores. Neste contexto, o presente estudo tem por objetivo identificar a produção do conhecimento latino-americano que trata da influência da inovação tecnológica nas cargas de trabalho dos profissionais de saúde. Realizou-se uma revisão integrativa, seleção de artigos, teses ou dissertações, publicados em periódicos indexados na base de dados LILACS nos últimos cinco anos. Foram encontradas onze publicações, as quais foram analisadas conforme procedência, tipo de estudo, objetivos das pesquisas, metodologia empregada, referencial teórico utilizado e resultados. Os resultados evidenciaram concentração das publicações no Brasil, pesquisas com abordagem qualitativa, realizadas eminstituições públicas e no ambiente hospitalar. Conclui-se que os temas inovação tecnológica e cargas de trabalho estão presentes na literatura latino-americana, no entanto não existem estudos que abordem o impacto do uso de novas tecnologias nas cargas de trabalho dos profissionais do setor.

  13. Colour, lipid and protein stability of Rhea americana meat during air- and vacuum-packaged storage: influence of muscle on oxidative processes.

    Filgueras, R S; Gatellier, P; Aubry, L; Thomas, A; Bauchart, D; Durand, D; Zambiazi, R C; Santé-Lhoutellier, V

    2010-11-01

    Physicochemical characteristics and oxidative stability during storage were determined in Gastrocnemius pars interna (GN) and Iliofiburalis (IF) muscles of Rhea americana. Glycolytic potential (GP) and pH decline of muscles were measured within the first 24 h post mortem. Colour, lipid and protein stability were determined during storage of meat, i.e. 5 days under air-packaging at 4°C, or 28 days under vacuum-packaging at 4°C. In parallel, anti-oxidant status of muscles was estimated by measuring α-tocopherol content and anti-oxidant enzyme activities (superoxide dismutase and catalase), while pro-oxidant status was evaluated by determining haeminic iron and long chain fatty acids (especially polyunsaturated fatty acids). The ultimate pH was similar in both muscles, but the GP value was significantly higher in IF than in GN muscle. Haeminic iron and alpha-tocopherol content differed between muscles, with 30% more haeminic iron (ppackaging, lipid and protein oxidation of rhea muscles increased up to 275% and 30%, respectively. This increase was more rapidly and marked in IF muscle. The IF also showed high level of metmyoglobin accumulation after 3 days of storage (47%) and was rejected by 1 consumer out of 2 in sensorial analysis. Under vacuum-packaging, both muscles showed a high stability of colour and no oxidation of lipids and proteins.

  14. A third type of raphide crystal in the plant kingdom: Six-sided raphides with laminated sheaths in Agave americana L.

    Wattendorff, J

    1976-01-01

    Raphides in leaves of Agave americana L. have six-sided cross sections. Each crystal tapers off to a point at both ends. It is enveloped in a 100 nm thick sheath which, in cross section, shows lamellae with periods of 6-9 nm. No polysaccharides could be detected in the sheaths with the Thiéry reaction. Dissolution of a raphide in acid occurs slowly from both ends leaving the crystal sheath visible in the light microscope. The raphide cell walls contain a layer that in glutaraldehyde-fixed tissue reacts neither with the Thiéry stain nor with potassium permanagnate. Its morphology resembles the "isotropic layer" of Chafe and Chauret (Protoplasma 80, 129-147, 1974) but no lignification could be shown as yet. Though up to now only raphides with four-sided or H-shaped cross sections have been observed by electron microscopy, we suggest that many raphides described as "rounded" in light microscopy might in fact be six-sided.

  15. Leishmaniose tegumentar americana: casuística hospitalar no Rio de Janeiro American tegumentary Leishmaniasis: hospitalized cases in Rio de Janeiro

    Nurimar C. Fernandes

    2004-08-01

    Full Text Available FUNDAMENTOS: A leishmaniose tegumentar americana distribui-se amplamente no Brasil, e o Estado do Rio de Janeiro (capital e interior constitui área endêmica onde o vetor é encontrado dentro e ao redor das habitações. OBJETIVOS: Análise prospectiva de 48 casos de leishmaniose tegumentar americana atendidos no Hospital Universitário Clementino Fraga Filho/UFRJ, no período de 1990 a 2002. MÉTODOS: Todos os pacientes foram submetidos à biópsia de pele ou mucosa, teste de Montenegro e exame otorrinolaringológico; SbV (10 a 20mg/kg, no total de 10, 30 e 90 doses. foi empregado em 44 pacientes; em quatro casos, anfotericina B (0,5mg/kg/dose até dose cumulativa de 30mg/kg. RESULTADOS: 28 homens e 20 mulheres na faixa etária de 10 a 89 anos, dos quais 38 (79,1% infectados no Rio de Janeiro, apresentaram úlcera de membro inferior e comprometimento de mucosa nasal como manifestações mais freqüentes; 41 casos (85,4% foram reatores à intradermorreação de Montenegro (5mm; 17 casos (35,4% foram positivos para o achado de amastigotas em macrófagos no infiltrado inflamatório dérmico; predominou o processo inflamatório crônico granulomatoso; cura clínica foi observada em 47 casos; um caso evoluiu para óbito no décimo dia de tratamento. CONCLUSÕES: O estudo da leishmaniose tegumentar (HUCFF/UFRJ no período 1990/2002 evidenciou padrão conhecido nos aspectos epidemiológicos, clínicos e de resposta terapêutica ao antimonial (SbV e anfotericina B.BACKGROUND: American tegumentary Leishmaniasis is widely found in Brazil; the state of Rio de Janeiro (capital and hinterland is an endemic area where the vector is found inside and outside houses. OBJECTIVES: prospective study of 48 cases attended at the Teaching Hospital Clementino Fraga Filho - UFRJ, between 1990 - 2002. METHODS: All patients were submitted to skin or mucosa biopsy, Montenegro skin test and otorhinolaryngologic examination; SbV (10 - 20 mg/kg with 10, 30 and 90 doses was

  16. Science of breeding and heredity from ancient Persia to modern Iran.

    Kariminejad, Mohammad H; Khorshidian, Ardeshir

    2012-01-01

    About 1700 years BC, the prophet Zoroaster declared equal right for women and men to choose their "own ways." There is much evidence that ancient Persians believed in the equal contribution of women and men toward producing a child, and all its hereditary characteristics.Even more surprising are the phrases in Vandidad book, which were gathered by Mobedans in the Mad dynasty about egg extraction (gametes) from animal reproductive organs (gonads) and their storage for future conception.Centuries later, Western philosopher beliefs in regard to reproduction were contrary to Persian knowledge. The Greek philosophers believed that man's water (semen) contains all human characteristics, and the female uterus is only responsible for nurturing and development of fetus. After detection of the ovum (de Graaf 2(nd) half 17 century) Malpigy proposed the preformation theory (ovist) which means there is a miniature human inside ovum, that grows after Semen has entered the uterus and grow into a well-developed fetus. This hypothesis was later delegated to spermatozoa. These contradictory and inappropriate beliefs were subject to discussions and dispute, until C.E. Wolf demonstrated that the embryo is a product of the fertilization of ovum by spermatozoa.800 years prior this the sage Ferdowsi "The Great Iranian Poet" explains nicely the equal participation of man and woman in the production of the fetus and transmission of characters.After the renaissance and especially in recent years, tremendous achievements have been made in unraveling biological secrets of reproduction. There was no work o n genetics in Iran until 1936, when a genetic course was added to the biology curriculum in related colleges and universities; Iranian Genetics Society was founded in 1966, initiating a steady movement in this field.Although there was an inevitable gap during the revolution and war in our country, now there is great effort by researchers to eliminate the gap and bring us into the mainstream of world science, and development in biomedical sciences in the third millennium.

  17. Science of breeding and heredity from ancient Persia to modern Iran

    Mohammad H Kariminejad

    2012-01-01

    Although there was an inevitable gap during the revolution and war in our country, now there is great effort by researchers to eliminate the gap and bring us into the mainstream of world science, and development in biomedical sciences in the third millennium.

  18. 古波斯文明%The Civilization of Ancient Persia

    2004-01-01

    @@ Comparatively little is known of the Persians before the sixth century B.C. Up to that time they appear to have led an obscure and peaceful existence on the eastern shore of the Persian Gulf. Their homeland afforded only modest advantages. On the east it was hemmed in by high mountains, and its coast line was destitute of harbors. The fertile valleys of the interior, however, were capable of providing a generous subsistence for a limited population. Save for the development of an elaborate religion, the people had made little progress. They had no system of writing, but they did have a spoken language closely related to Sanskrit and to the languages of ancient and modern Europe. It is for this reason alone and not because of race that they are accurately referred to as an Indo-European people. At the dawn of their history they were not an independent nation but were vassals1 of Medes, a kindred2 people who ruled over a great empire north and east of the Tigris River.

  19. Surgeons and surgery from ancient Persia (5,000 years of surgical history).

    Zargaran, Arman; Fazelzadeh, Afsoon; Mohagheghzadeh, Abdolali

    2013-08-01

    The development of surgery is indebted to scientists and surgeons from various civilizations throughout history. The present study considers surgery in the ancient Persian civilization. It highlights aspects of the subject, such as findings of the first trephinated skulls in Iran; surgeons' social class (kareto baēšaza in the Avestan language-dating back 3,000 years); surgical operations such as cesarean section and procedures to treat breast cancer; and the use of anesthetic compounds and surgical practice in the military. It is hoped that this catalogue of historical evidence of surgical practice in ancient Persian civilization will contribute to the history of surgery, as an important field in medical science.

  20. Leishmaniose tegumentar americana na Ilha Grande. Rio de Janeiro: V. Observações sobre a biologia dos transmissores em condiçoes naturais

    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Durante um surto de Leishmaniose Tegumentar Americana (LTA na Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, foram coletados 4.192 exemplares de flebotomíneos em ambiente domiciliar e silvestre, com isca animal e outros abrigos naturais, durante o período de janeiro de 1976 a abril de 1977. Entre o total de 11 espécies coletadas encontraram-se 2.493 exemplares de Lutzomyia intermedia, 1.594 de Lutzomyia migonei e 105 exemplares de outras espécies. A L. intermedia apresentou densidade mais elevada no domicílio humano, enquanto a L. migonei apresentou coletas mais rendosas com iscas animais e galinheiros. O sinantropismo e a elevada densidade no ambiente domiciliar incriminam as espécies L. intermedia e L. migonei como as prováveis vetoras da LTA na Ilha Grande.During an outbreak of mucocutaneous leishmaniasis in Praia Vermelha, Ilha Grande (Great Island in the county of Angra dos Reis, State of Rio de Janeiro, 4.192 species of Phlebotomus were collected in domestic and wild environment with the use of animal baits and other natural shelters from January 1976 to April 1977. In a total of 11 species collected, 2.493 samples of Lutzomyia intermedia, 1.594 of Lutzomyia migonei and 105 samples of other species were found. The Lutzomyia intermedia presented a higher density in human dwellings, while the Lutzomyia migonei presented better results with animal baits and chicken-coops. The sinantropism and high density found in the houses incriminate the species L. intermedia and L. migonei as the probable vectors of Mucocutaneous Leishmaniasis in Ilha Grande.

  1. Aspectos sorológicos e epidemiológicos da leishmaniose tegumentar americana canina em Maricá, Rio de Janeiro, Brasil

    Uchôa Claudia Maria Antunes

    2001-01-01

    Full Text Available Foram estudados 261 cães (134 moradias do Município de Maricá, RJ, Brasil, visando avaliar a resposta sorológica e infecção ativa para leishmaniose tegumentar americana (LTA. Oito cães apresentaram lesões sugestivas, sendo o isolamento positivo em 3. Pelo ELISA, 24,5% (64/261 apresentaram reatividade (sensibilidade = 66% e especificidade = 76%, estando associado ao isolamento em 2 e 0,4% (1/261 pela imunofluorescência indireta (RIFI sem associação com isolamento. Para a redução de reações inespecíficas no ELISA, utilizou-se um segundo critério para obtenção do cutoff (sensibilidade = 33% e especificidade = 93%, obtendo positividade de 6,9% (18/261 associando-se ao isolamento em um animal. Sete pessoas apresentaram cicatrizes de LTA e uma lesão ativa em tratamento. A não associação da infecção ativa dos cães à sorologia pela IFI e o grande número de resultados inespecíficos encontrado pelo ELISA, restringe o uso isolado destas no diagnóstico precoce da LTA. O encontro de lesões ativas confirma a circulação recente da Leishmania em Maricá, indicando a necessidade de estudos nesta região.

  2. Effect of cold storage on larval and adult Anastrepha ludens (Diptera: Tephritidae) viability in commercially ripe, artificially infested Persea americana 'Hass'.

    Aluja, M; Díaz-Fleischer, F; Arredondo, J; Valle-Mora, J; Rull, J

    2010-12-01

    Commercially ripe 'Hass' avocados, Persea americana Mill, artificially exposed to wild Anastrepha ludens (Loew) (Diptera: Tephritidae) females 24 h after harvest were placed in a cold storage facility to determine the effect of low temperature on larval survival and adult viability. Fruit were left for 3, 6, 9, and 12 d in a cold room at 5 degrees C followed by a 20-25-d period at ambient temperature to allow for larval development and pupation. Hass avocados and grapefruit, Citrus paradisi Macfadyen, maintained at ambient temperature served as controls. Overall, only 0.23% of the Hass avocados and 19.30% of the grapefruit were infested. The number of infested fruit increased with decreasing exposure time to cold. Puparia from cold-treated Hass avocados were significantly smaller than those stemming from cold-treated grapefruit. Hass avocados exposed for 12 d to 5 degrees C yielded no puparia, and those exposed for 6 and 9 d yielded 22 and two puparia, respectively, but no adults. Although Hass avocados exposed to cold temperature for 3 d yielded adults that reached sexual maturity (N = 16), females laid inviable eggs. Grapefruit exposed to cold for 12 d yielded normal-sized puparia (but no adults), whereas those exposed over 9 d yielded females able to lay viable eggs. We conclude that exposing fruit to cold storage after packing and during transport represents an effective risk-mitigating procedure in the highly improbable event that a gravid A. ludens female might lay eggs in a commercially ripe Hass avocado that had been left unprotected in a packinghouse.

  3. Dietas artificiais para a criação de larvas e adultos da mosca-das-frutas sul-americana

    Adrise Medeiros Nunes

    2013-10-01

    Full Text Available O objetivo deste trabalho foi adequar as dietas artificiais para o desenvolvimento dos estágios de larva e adulto da mosca-das-frutas sul-americana (Anastrepha fraterculus. Para o estágio larval, foram testadas as seguintes dietas: D1, original, com 10 g de ágar; D2, modificada, com 3,6 g de ágar; e, D3, modificada, com bagaço seco de cana-de-açúcar. Para os adultos, foram testadas quatro dietas: A, levedura de cerveja + mel (2:1; B, açúcar refinado + extrato de levedura + gérmen de trigo cru (3:1:1; C, extrato de soja + açúcar mascavo + gérmen de trigo cru (3:1:1; e D, levedura seca de cervejaria + mel (2:1. Avaliaram-se os parâmetros biológicos de duração do período ovo-pupa, duração e viabilidade do estágio de pupa, massa média de pupas, razão sexual e duração e viabilidade do período ovo-adulto. O desenvolvimento larval em D1 e D2 foi semelhante e indicou que a criação de larvas pode ser realizada com 1/3 da quantidade de ágar da utilizada em D1. A utilização do bagaço seco de cana-de-açúcar, na dieta artificial, afetou negativamente o desenvolvimento larval. As dietas artificiais com levedura de cerveja + mel e com açúcar refinado + extrato de levedura + gérmen de trigo cru são as mais adequadas para a criação de adultos.

  4. A new record for American Bullfrog (Lithobates catesbeianus in San Juan, Argentina Nuevo registro de rana toro americana (Lithobates catesbeianus en San Juan, Argentina

    Eduardo Sanabria

    2011-03-01

    Full Text Available We report a new record of Lithobates catesbeianus (American bullfrog from Argentina. L. catesbeianus was first introduced to San Juan Province 11 years ago in Calingasta Department, where the habitat is pre-cordilleran. The new record is for Zonda Department, San Juan Province, in the Monte desert region. Here, L. catesbeianus uses artificial ponds for reproduction and tadpole development. These ponds receive water from an irrigation system that connects the whole agriculture land in the region. The tadpoles use the irrigation canals to move among ponds. We suggest that legislation should be established to prevent future invasions and to achieve sustainable management of the wild American bullfrog populations in San Juan. Prevention of future invasion and management of established populations of this species requires the cooperation of numerous stake holders.Se presenta un nuevo registro de Lithobates catesbeianus (rana toro americana en Argentina. L. catesbeianus fue introducida por primera vez a la provincia de San Juan hace 11 años en el Departamento Calingasta, donde el hábitat es pre-cordillerano. El nuevo registro es para el Departamento Zonda en la provincia de San Juan, en el desierto del Monte. En este sitio, L. catesbeianus usa estanques artificiales para la reproducción y desarrollo del renacuajo. Los estanques reciben agua de un sistema de riego que conecta todas las tierras de la agricultura en la región. Los renacuajos utilizan los canales de riego para moverse entre los estanques. Sugerimos que se establezcan leyes para prevenir invasiones futuras y para lograr un manejo integrado de las poblaciones silvestres de rana toro que se encuentran en San Juan. La prevención de futuras invasiones y el manejo de las poblaciones establecidas de esta especie requieren la cooperación de numerosas entidades tanto gubernamentales como privadas.

  5. Colocalization of allatotropin and tachykinin-related peptides with classical transmitters in physiologically distinct subtypes of olfactory local interneurons in the cockroach (Periplaneta americana).

    Fusca, Debora; Schachtner, Joachim; Kloppenburg, Peter

    2015-07-01

    In the insect antennal lobe different types of local interneurons mediate complex excitatory and inhibitory interactions between the glomerular pathways to structure the spatiotemporal representation of odors. Mass spectrometric and immunohistochemical studies have shown that in local interneurons classical neurotransmitters are likely to colocalize with a variety of substances that can potentially act as cotransmitters or neuromodulators. In the antennal lobe of the cockroach Periplaneta americana, gamma-aminobutyric acid (GABA) has been identified as the potential inhibitory transmitter of spiking type I local interneurons, whereas acetylcholine is most likely the excitatory transmitter of nonspiking type IIa1 local interneurons. This study used whole-cell patch clamp recordings combined with single-cell labeling and immunohistochemistry to test if the GABAergic type I local interneurons and the cholinergic type IIa1 local interneurons express allatotropin and tachykinin-related neuropeptides (TKRPs). These are two of the most abundant types of peptides in the insect antennal lobe. GABA-like and choline acetyltransferase (ChAT)-like immunoreactivity were used as markers for GABAergic and cholinergic neurons, respectively. About 50% of the GABA-like immunoreactive (-lir) spiking type I local interneurons were allatotropin-lir, and ∼ 40% of these neurons were TKRP-lir. About 20% of nonspiking ChAT-lir type IIa1 local interneurons were TKRP-lir. Our results suggest that in subpopulations of GABAergic and cholinergic local interneurons, allatotropin and TKRPs might act as cotransmitters or neuromodulators. To unequivocally assign neurotransmitters, cotransmitters, and neuromodulators to identified classes of antennal lobe neurons is an important step to deepen our understanding of information processing in the insect olfactory system.

  6. Casos de leishmaniose tegumentar americana por Leishmania (viannia braziliensis nos municípios de cosmópolis e Indaiatuba - região de Campinas, estado de São Paulo, Brasil

    Selma Giorgio

    1996-10-01

    Full Text Available Com o objetiiro de identificar a espécie de Leishmania envolvida nas afecções cutâneas de indivíduos residentes nos municípios de Cosmópolis e Indaiatuba, região de Campinas, Estado de São Paulo, correlacionamos dados clínicos, histopatológicos e testes de bibridização in situ. Os expressivos índices de incidência de leishmaniose tegumentar americana obsewados nesses municípios nos levaram a iniciar esses estudos. Nesse trabalho, apresentamos os dados relativos a seis indivíduos. As características das lesões, ulceradas, de dificil cicatrização e presentes em locais expostos do corpo sugeriram quadros de leishmaniose tegumentar americana. Os testes de intradermoreação de Montenegro, positivos em cinco dos seis pacientes analisados também reforçaram a suspeita de leishmaniose tegumentar americana. Os padrões histopatológicos, como reação exsudativa celular e granulomatosa e a dificuldade de isolamento dos parasitas obtidos de biópsias de lesões são compatíveis com aqueles descritos para Leishmania (Viannia braziliensis. Em testes de bibridização in situ do DNA do cinetoplasto de amastigotas das lesões dos seis pacientes, obseivamos que quatro deles apresentavam sinais de bibridização com a sonda de L. (Viannia braziliensis, confirmando as suspeitas de que a Leishmania responsável pelas afecções cutâneas nos pacientes analisados era do subgenêro Viannia e do complexo braziliensis.

  7. Elevated CO2 increases Cs uptake and alters microbial communities and biomass in the rhizosphere of Phytolacca americana Linn (pokeweed) and Amaranthus cruentus L. (purple amaranth) grown on soils spiked with various levels of Cs.

    Song, Ningning; Zhang, Ximei; Wang, Fangli; Zhang, Changbo; Tang, Shirong

    2012-10-01

    General concern about increasing global atmospheric CO(2) levels owing to the ongoing fossil fuel combustion and elevated levels of radionuclides in the environment, has led to growing interest in the responses of plants to interactive effects of elevated CO(2) and radionuclides in terms of phytoremediation and food safety. To assess the combined effects of elevated CO(2) and cesium contamination on plant biomass, microbial activities in the rhizosphere soil and Cs uptake, Phytolacca americana Linn (pokeweed, C3 specie) and Amaranthus cruentus L. (purple amaranth, C4 specie) were grown in pots of soils containing five levels of cesium (0, 100, 300, 500 and 1000 mg Cs kg(-1)) under two levels of CO(2) (360 and 860 μL L(-1), respectively). Shoot and root biomass of P. americana and Amaranthus crentus was generally higher under elevated CO(2) than under ambient CO(2) for all treatments. Both plant species exhibited higher Cs concentration in the shoots and roots under elevated CO(2) than ambient CO(2). For P. americana grown at 0, 100, 300, 500 and 1000 mg Cs kg(-1), the increase magnitude of Cs concentration due to elevated CO(2) was 140, 18, 11, 34 and 15% in the shoots, and 150, 20, 14, 15 and 19% in the roots, respectively. For A. cruentus, the corresponding value was 118, 28, 21, 14 and 17% in the shoots, and 126, 6, 11, 17 and 22% in the roots, respectively. Higher bioaccumulation factors were noted for both species grown under elevated CO(2) than ambient CO(2). The populations of bacteria, actinomycetes and fungi, and the microbial C and N in the rhizosphere soils of both species were higher at elevated CO(2) than at ambient CO(2) with the same concentration of Cs. The results suggested that elevated CO(2) significantly affected plant biomass, Cs uptake, soil C and N concentrations, and community composition of soil microbes associated with P. americana and A. cruentus roots. The knowledge gained from this investigation constitutes an important advancement in

  8. Estilos de comunicación de los padres y su relación con la conducta de los estudiantes del sexto grado del nivel primario del Colegio Adventista Americana de la ciudad de Juliaca, 2012.

    Quispe Gutierrez, Edy Eucler

    2014-01-01

    La presente investigación corresponde a un diseño transeccional, de tipo descriptivo, correlacional. El objetivo principal es determinar la relación entre los estilos de comunicación de los padres y la conducta de los estudiantes del sexto grado del nivel primario del Colegio Adventista Americana de Juliaca, 2012, se trabajó con 97 alumnos y 97 padres de familia. Para medir los estilos de comunicación se utilizó el Inventario de Estilos de Comunicación que se basa ...

  9. Ocorrência de Aprostocetus hagenowii (Hymenoptera, Eulophidae, parasitoide de ootecas da barata americana, no Rio Grande do Sul Occurrence of Aprostocetus hagenowii (Hymenoptera, Eulophidae, parasitoid of the american cockroach oothecae at Rio Grande do Sul

    Marcial Corrêa Cárcamo

    2009-12-01

    Full Text Available Relata-se a ocorrência do parasitoide Aprostocetus hagenowii (Ratzeburg, 1952 (Hymenoptera: Eulophidae em ooteca de Periplaneta americana Linnaeus, 1758 (Blattodea: Blattidae, no extremo Sul do Brasil. As ootecas foram coletadas no mês de dezembro de 2007, no campus da Universidade Federal de Pelotas (31°48'34"S, 52°25'42"O, Município do Capão do Leão, Rio Grande do Sul. Após a coleta, as ootecas foram acondicionadas individualmente em tubos de ensaio, sendo posteriormente mantidas em câmara climatizada a 25°C, com umidade relativa =70%, até a eclosão das ninfas ou emergência dos parasitoides. Uma das ootecas estava parasitada e apresentou 89 parasitoides (79 fêmeas e 10 machos; a referida ocorrência constitui o primeiro registro para o Sul do Brasil. O conhecimento das regiões de ocorrência dos inimigos naturais de P. americana é de grande importância para se traçar uma estratégia de controle das populações desse blatódeo.It is reported the occurrence of the parasitoid Aprostocetus hagenowii (Ratzeburg, 1952 (Hymenoptera: Eulophidae in oothecae of Periplaneta americana Linnaeus, 1758 (Blattodea: Blattidae at the extreme southern Brazil. The oothecae were collected in December of 2007 at the campus of the Universidade Federal de Pelotas (31°48'34"S, 52°25'42"W, city of Capão do Leão, Rio Grande do Sul. After the collection the oothecae were placed individually in glass vials maintained in acclimatized chamber at 25°C, with relative air humidity =70% until the eclosion of the nymphs or the emergence of the parasitoids. The infested ootheca presented 89 parasitoids (79 females and 10 males. The referred occurrence represents the first report to southern Brazil. Knowing the regions of occurrence of the natural enemies of P. americana is of great importance when developing a control strategy to the populations of the blatod.

  10. [Dissemination of German medicine in Spain and Latin America: the "Revista Médica de Hamburgo" and the "Revista Médica Germano-Ibero-Americana" (1920-1933)].

    Sá, Magali Romero; Cândido da Silva, André Felipe

    2010-01-01

    This article presents the development of the journals "Revista Médica de Hamburgo" and "Revista Médica Germano-Ibero-Americana," which were created to promote and disseminate the German science among the medical community in Latin America and Spain between the two World Wars. Shaken by the loss of Germany's colonies in Africa, the difficulties faced due to post-war economy, and the restrictions imposed by the armistice, the Germans sought to restore their cultural and scientific prestige through such initiative.

  11. Forma mucosa da Leishmaniose Tegumentar Americana : estudo histopatológico e imuno-histoquímico de casos do Hospital Universitário de Brasília

    2009-01-01

    A Leishmaniose Tegumentar Americana (LTA) é uma doença parasitária endêmica, principalmente na região Centro-Oeste. A Leishmania é um parasita que através de seus antígenos secretados e não secretados desencadeiam, respectivamente, uma resposta imune predominantemente celular (inata e adquirida) e em menor proporção humoral. Na resposta inata participam o sistema complemento, polimorfonucleares, macrófagos e células natural killer numa resposta inicial. Desta resposta inicial, há produção de ...

  12. Guerra i persuasió. La justificació de l’acció militar en la retòrica presidencial nord-americana: de Vietnam a Afganistan

    2013-01-01

    El tema d'aquesta tesi és l'ús del discurs polític coma eina per influir en l'opinió pública, en concret la retòrica presidencial de guerra nord-americana i els recursos argumentatius que els presidents utilitzen en els seus discursos orals per persuadir els ciutadans de la necessitat de dur el país a la guerra. S'ha estudiat la retòrica presidencial per justificar la guerra del Vietnam i la guerra contra el terror (Afganistan i Iraq), partint de tres hipòtesis: 1) que existeix una continu!...

  13. Histopatología, fisiología y calidad postcosecha de frutos de aguacate (Persea americana Mill.) cv. 'Hass' infectados con el avocado sunblotch viroid (ASBVd) y diagnóstico de la enfermedad.

    Vallejo Pérez, Moisés Roberto

    2011-01-01

    Frutos de aguacate (Persea americana Mill) cv. ‘Hass’ se cosecharon de árboles infectados con el Avocado sunblotch viroid (ASBVd) en Tingambato, Michoacán, considerando tres condiciones fenotípicas: árboles asintomáticos vigorosos (A), árboles de vigor regular (AR) y árboles con síntomas en frutos (S). Se determinó la presencia del ASBVd mediante RT-PCR e hibridación Northern con ribosondas complementarias al viroide. La presencia del ASBVd se detectó en el exocarpio y mesocarpio de frutos de...

  14. 金边龙舌兰一种新病害的病原菌鉴定%Pathogen identification of a new disease on Agave americana L. var. marginata Hort

    黄玖利; 张荣意; 谭志琼

    2008-01-01

    对海南省金边龙舌兰(Agave americana L. var.marginata Hort)的一种新病害进行了症状描述和病原菌鉴定,结果表明:第一病原是肺腐镰刀菌[Fusarium solani(Mart.Sacc.]和燕麦镰刀菌[Fusarium avenaceum(Fr.)Sacc.],第二病原是果胶杆菌(Pectobacterium sp.),可增强茄腐镰刀菌对金边龙舌兰的致病性.

  15. La traducción al español de la prosa nativo-americana: estudio crítico de la (re)creación transcultural de la identidad indígena estadounidense

    Herrero López, Isis

    2013-01-01

    [ES]Debido a la complejidad socio-artística de Estados Unidos, el mundo académico español apenas ha explorado las producciones literarias de las comunidades nativo-americanas. mucho menos desde un punto de vista traductológico. Esto supone que las traducciones disponibles en nuestro país no han sido estudiadas críticamente hasta la fecha, un hecho que esta Tesis Doctoral compensa a través de un análisis multidisciplinar y multifocal. A lo largo de la Tesis Doctoral, se ha observado la li...

  16. Detecção de DNA de Leishmania braziliensis em pacientes de leishmaniose tegumentar americana Detección de DNA de Leishmania braziliensis en pacientes de leishmaniose tegumentaria americana Detection of Leishmania braziliensis DNA in American tegumentary leishmaniasis patients

    Leila Martins

    2010-06-01

    Full Text Available Foi realizado diagnóstico para leishmaniose tegumentar americana a partir de sangue de pacientes residentes em dois municípios endêmicos do estado de Pernambuco. O DNA de 119 amostras de sangue foi extraído e submetido a reação em cadeia da polimerase. Utilizaram-se primers do minicírculo do DNA do cinetoplasto (kDNA de Leishmania braziliensis, circulante em Pernambuco, cuja seqüência-alvo gera um fragmento de 750 pares de bases. No total 58 (48,7% indivíduos apresentaram amplificação positiva e 61 (51,3% negativa. Das amostras positivas para a PCR, 37 (≅ 64% pertenciam a indivíduos tratados e sem lesão. Conclui-se que a técnica de PCR é eficaz para identificar o DNA de leishmânia em material de biópsias e em sangue venoso.Fue realizado diagnóstico para leishmaniosis tegumentaria americana a partir de sangre de pacientes residentes en dos municipios endémicos del estado de Pernambuco (Noreste de Brasil. El DNA de 119 muestras de sangre fue extraído y sometido a la reacción en cadena de la polimerasa. Se utilizaron primers del minicírculo del DNA del cinetoplasto (kDNA de Leishmania braziliensis, circulante en Pernambuco, cuya secuencia blanco genera un fragmento de 750 pares de bases. En total 58 (48,7% individuos presentaron amplificación positiva y 61 (51,3% negativa. De las muestras positivas para la PCR, 37 (≅64% pertenecían a individuos tratados y sin lesión. Se concluyó que la técnica de la PCR es eficaz para identificar el DNA de Leishmania en material de biopsias y en sangre venosa.Diagnostic tests for American tegumentary leishmaniasis were performed on blood samples of patients living in two endemic municipalities in the state of Pernambuco, Northeastern Brazil. DNA was extracted from 119 samples and used as template for polymerase chain reaction (PCR analysis. The tests used primers specific for the kinetoplast mini-circle DNA (kDNA of Leishmania braziliensis, a species circulating in Pernambuco, which

  17. Caracterização química das plantas aquáticas coletadas no reservatório de Salto Grande (Americana-SP Chemical characterization of aquatic plants assessed in Salto Grande reservoir (Americana-SP, Brazil

    D. Martins

    2003-01-01

    Full Text Available O objetivo deste trabalho foi caracterizar a constituição química das espécies de plantas aquáticas Brachiaria arrecta, Eichhornia crassipes, Pistia stratiotes e Salvinia auriculata - encontradas no reservatório da usina hidrelétrica de Salto Grande, em Americana-SP - de forma a fornecer subsídios para futuras avaliações sobre o comportamento da biomassa dessas espécies em local de descarte ou no próprio reservatório. As amostras de plantas foram coletadas no dia 16.4.2002, sendo desidratadas em estufa de circulação forçada de ar a 60 ºC. B. arrecta apresentou os menores teores médios de macro e micronutrientes e o maior teor médio de elementos pesados na matéria seca, em relação às demais espécies. A relação C/N das espécies E. crassipes, P. stratiotes e S. auriculata apresentou valores próximos. Não foi detectada, em nenhuma das espécies estudadas, a presença dos elementos molibdênio, prata, chumbo e mercúrio.This research aimed to describe the chemical components of the following aquatic plants: Brachiaria arrecta, Eichhornia crassipes, Pistia stratiotes and Salvinia auriculata, found in a reservoir formed by Salto Grande power plant in Americana-SP, Brazil, to provide a basis for future evaluations of the biomass behavior of these species at waste points or in the reservoir. Plant samples were collected on 04/16/2002. B. arrecta showed the lowest medium contents of macro and micronutrients and the highest medium contents of heavy metal in the dry matter, compared to the other aquatic species. The C/N relationship of E. crassipes, P. stratiotes and S. auriculata showed similar contents. Molybdenum, silver, lead and mercury were not detected in any of the species studied.

  18. Estudo da degradação da biomassa de três espécies de plantas aquáticas no reservatório da UHE de Americana-SP Study on the degradation of three aquatic weeds at the Americana-SP reservoir in Brazil

    E. Negrisoli

    2006-06-01

    Full Text Available O estudo de decomposição de plantas aquáticas foi realizado na UHE de Americana-CPFL, com o objetivo de avaliar a taxa de degradação de plantas na própria água do reservatório. Foram consideradas como variáveis a profundidade (superfície, 3,5 e 7,0 m, as espécies de plantas aquáticas (Eichhornia crassipes, Brachiaria subquadripara e Pistia stratiotes e o tipo de processamento a que estas foram submetidas (planta inteira, planta picada manualmente e com aplicação de fogo. Este estudo indicou que as espécies E. crassipes e P. stratiotes foram rapidamente decompostas no reservatório, ao contrário de B. subquadripara. Um outro fator importante observado foi a similaridade na decomposição das plantas, independentemente da profundidade testada. Essas diferenças devem ser consideradas na elaboração de planos de manejo e na previsão do impacto ambiental de programas de controle de plantas aquáticas.A study on aquatic weed decomposition was carried out at the Americana-SP reservoir in Brazil, to compare degradation rate of the plants in the reservoir's water itself. Three variables were considered as treatments: depth (surface, 3.5 and 7.0 m; aquatic weed species (Eichhornia crassipes, Brachiaria subquadripara, and Pistia stratiotes and type of handling the aquatic weeds (whole plant, hand cut plant, and burnt plant. The results indicated that the species E. crassipes and P. stratiotes had a fast decomposition in the reservoir, unlike B. subquadripara. These differences must be considered in the elaboration of management plans and prediction of environmental impact of aquatic plant control programs. The most positive aspect of the study was to demonstrate that decomposition at 3.5m and 7.0 m of depth had practically the same speed observed on the surface.

  19. Leishmaniose tegumentar americana: estudo clínico, epidemiológico e laboratorial realizado no Hospital Universitário de Campo Grande, Mato Grosso do Sul, Brasil American cutaneous leishmaniasis: clinical, epidemiological and laboratory studies conducted at a university teaching hospital in Campo Grande, Mato Grosso do Sul, Brazil

    Nathalia Dias Negrão Murback; Günter Hans Filho; Roberta Ayres Ferreira do Nascimento; Katia Regina de Oliveira Nakazato; Maria Elizabeth Moraes Cavalheiros Dorval

    2011-01-01

    FUNDAMENTOS: Leishmaniose Tegumentar Americana é zoonose de manifestações clínicas variadas, em expansão no Brasil, sendo o estado de Mato Grosso do Sul importante área endêmica. OBJETIVOS: Avaliar clínica, epidemiológica e laboratorialmente pacientes com Leishmaniose Tegumentar Americana , atendidos no Hospital Universitário Maria Aparecida Pedrossian, Universidade Federal de Mato Grosso do Sul, Brasil (HU/UFMS). MÉTODOS: Trata-se de um estudo observacional do tipo transversal com abordagem ...

  20. Aspectos clínicos e diagnósticos da leishmaniose tegumentar americana em militares simultaneamente expostos à infecção na Amazônia

    Guerra Jorge Augusto de Oliveira

    2003-01-01

    Full Text Available Os treinamentos militares na selva amazônica constituem importantes fatores na incidência de leishmaniose tegumentar americana na região. Este trabalho descreve 48 casos de leishmaniose tegumentar americana em pacientes militares nos quais a doença se manifestou após operação de treinamento na Amazônia. O período de incubação médio foi de 27,6 dias. Houve predomínio de lesões nos membros superiores, face e pescoço. A maioria dos indivíduos apresentou duas lesões, sendo as úlceras as manifestações mais freqüentes. O diagnóstico foi realizado pelo exame de escarificação das lesões, sendo confirmado pela demonstração do parasito em 43 (89,6% casos.

  1. ESTATUS DEL NO NACIDO EN LA CONVENCIÓN AMERICANA: UN EJERCICIO DE INTERPRETACIÓN Status of the unborn in the American convención: an interpretive exercise

    Álvaro Paúl Díaz

    2012-01-01

    Full Text Available Este estudio interpreta el ambiguo artículo 4.1 de la Convención Americana sobre Derechos Humanos, que dispone que el derecho a la vida deberá protegerse, "en general, a partir del momento de la concepción". Al hacerlo, toma en cuenta diversos sistemas de interpretación, y tiene presente lo registrado en los trabajos preparatorios de la Convención. Asimismo, este estudio analiza lo que la Comisión Interamericana ha resuelto en esta materia, evaluando el valor de tales decisiones. Este artículo concluye que, si bien una de las posibles interpretaciones de la Convención Americana sostiene que ella toleraría ciertas legislaciones nacionales que permitan el aborto en circunstancias excepcionales, ella declara la personalidad del nasciturus.This study interprets the ambiguous Article 4(1 of the American Convention on Human Rights, which establishes that life shall be protected "in general, from the moment of conception". When doing so, it pays attention to different interpretive systems, and takes into account what is recorded in the travaux préparatoires of the Convention. Likewise, this study analyzes what the Inter-American Commission has determined on this issue, and assesses the value of those decisions. This article concludes that, even though one of the possible interpretations of the American Convention affirms that it would tolerate domestic legislations providing for abortion in exceptional circumstances, it declares the unborn's personhood.

  2. A religião civil americana na construção de Superman e dos super-heróis = The american civil religion in the Superman and super heroes construction

    Flor, Ricardo Bruno

    2013-01-01

    Full Text Available O presente artigo visa verificar e explicar a influência da religião civil americana, como entendida por Catroga e Bellah, sobre o processo que originou os super-heróis, mais especificamente o personagem Superman; focando-se em como a crença na frontier (enquanto articulação da crença no manifest destiny ajudou a construir as características excepcionais do personagem marcando os super-heróis a partir dele. São abordados, tanto na literatura americana como em suas histórias em quadrinhos (tirinhas e revistas, os gêneros western, policial-detetivesco e aventura (aventura espacial, selvagem e outras variações, bem como os peculiares personagens pré-super-heroicos e as primeiras histórias do próprio Superman. O objetivo é mostrar como as características dos super-heróis e do próprio Superman foram inseridas e mantidas nesses gêneros ficcionais pela influência da crença na frontier, explicando a origem do personagem (e dos super-heróis como uma construção gradual

  3. Dissemination of German Medicine in Spain and Latin America:The Revista Médica de Hamburgo and the Revista Médica Germano-Ibero-Americana (1920-1933

    Romero Sá, Magali

    2010-06-01

    Full Text Available This article presents the development of the journals Revista Médica de Hamburgo and Revista Médica Germano-Ibero-Americana, which were created to promote and disseminate the German science among the medical community in Latin America and Spain between the two World Wars. Shaken by the loss of Germany’s colonies in Africa, the difficulties faced due to post-war economy, and the restrictions imposed by the armistice, the Germans sought to restore their cultural and scientific prestige through such initiative.

    El trabajo aborda la trayectoria de la Revista Médica de Hamburgo (RMH y la Revista Médica Germano-Ibero-Americana (RMGIA, publicaciones creadas para promover y difundir la ciencia alemana entre las comunidades médicas de América Latina y España en el contexto de entreguerras. Sacudidos por la pérdida de las colonias en África, por las dificultades enfrentadas por la economía y por las condiciones impuestas por el armisticio, los alemanes pretendían con esa iniciativa recuperar el prestigio cultural y científico.

  4. Localização encefálica da blastomicose sul-americana. Considerações a propósito de 9 casos

    Walter C. Pereira

    1965-06-01

    Full Text Available Lesões encefálicas produzidas pelo Paracoccidioides brasiliensis têm sido registradas na literatura com relativa freqüência. Os autores chamam a atenção para a dificuldade diagnóstica dêstes processos que, na quase totali- dade, constituem-se de achados cirúrgicos ou de necropsia. A síndrome de hipertensão intracraniana é a manifestação mais comum, levando quase sempre ao diagnóstico de tumor intracraniano. Os exames complementares, inclusive o líquido cefalorraqueano, são pobres em dados específicos; o encontro do parasita, assim como a positividade das reações de fixação de complemento e de precipitação nesse humor, são raramente verificadas. A natureza da afecção só tem sido suspeitada quando existem lesões paracocci-dióicas conhecidas em outros órgãos. Nove casos de blastomicose sul-americana com localização encefálica são estudados. Sete foram submetidos a intervenções cirúrgicas, dêstes, 3 tiveram boa evolução, sendo os únicos que sobreviveram de tôda a série. Nos três casos foi empregada, em épocas diversas depois da intervenção cirúrgica, anfotericina B pelas vias intravenosa ou intratecal. Os autores consideram a excição cirúrgica dos granulomas encefálicos imprescindível, mormente quando existem sinais de compressão bem localizadas. Após o ato cirúrgico a administração de anfotericina B, pelas vias intravenosa ou intratecal, deve ser prescrita a fim de evitar possíveis recidivas da moléstia.

  5. Reduction of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes with electrolyzed oxidizing water on inoculated hass avocados (Persea americana var. Hass).

    Rodríguez-Garcia, O; González-Romero, V M; Fernández-Escartín, E

    2011-09-01

    This study was intended to evaluate the bactericidal effect of electrolyzed oxidizing water (EOW) and chlorinated water on populations of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes inoculated on avocados (Persea americana var. Hass). In the first experiment, inoculated avocados were treated with a water wash applied by spraying tap water containing 1 mg/liter free chlorine for 15 s (WW); WW treatment and then spraying sodium hypochlorite in water containing 75 mg/liter free chlorine for 15 s (Cl75); WW treatment and then spraying alkaline EOW for 30 s (AkEW) and then spraying acid EOW (AcEW) for 15 s; and spraying AkEW and then AcEW. In another experiment, the inoculated avocados were treated by spraying AkEW and then AcEW for 15, 30, 60, or 90 s. All three pathogen populations were lowered between 3.6 and 3.8 log cycles after WW treatment. The application of Cl75 did not produce any further reduction in counts, whereas AkEW and then AcEW treatment resulted in significantly lower bacterial counts for L. monocytogenes and E. coli O157:H7 but not for Salmonella. Treatments with AkEW and then AcEW produced a significant decrease in L. monocytogenes, Salmonella, and E. coli O157:H7 populations, with estimated log reductions of 3.9 to 5.2, 5.1 to 5.9, and 4.2 to 4.9 log CFU/cm², respectively. Spraying AcEW for more than 15 s did not produce any further decrease in counts of Salmonella or E. coli O157:H7, whereas L. monocytogenes counts were significantly lower after spraying AcEW for 60 s. Applying AkEW and then AcEW for 15 or 30 s seems to be an effective alternative to reduce bacterial pathogens on avocado surfaces.

  6. O papel da dramaturgia na leitura do imaginário cultural: reinterpretando a identidade latino-americana de um ponto de vista francês

    Geraldo Pontes Júnior

    2011-11-01

    Full Text Available Resumo: Análise de textos interpretativos do imaginário cultural latino-americano, do ensaio ao teatro, avaliando seus horizontes de abordagem, como forma de ponderar a capacidade de o processo criativo da escritura dramaturgia estabelecer uma interseção com a perspectiva de estudos culturais que os ensaios podem representar. Leitura comparativa da colonização mexicana (e, mais amplamente falando, latino-americana, através dos textos de Octavio Paz (O labirinto da solidão, de Eduardo Subirats (A penúltima visão do paraíso e do dramaturgo francês Michel Azama (Aztèques.Palavras-chave: Literatura comparada; literatura mexicana; Octavio Paz; O labirinto da solidão; Eduardo Subirats; A penúltima visão do paraíso; literatura francesa; Michel Azama; Aztecas.Résumé: Analyse de textes interprétatifs de l’imaginaire culturel latino-américain, de l’essai au théâtre, dans une évaluation de leurs horizons d’approche comme moyen de pondérer l’efficace du processus créatif de l’écriture dramaturgique dans études cuturelles que les essais peuvent représenter. Lecture comparative de la colonisation mexicaine (et, pour parler plus amplement, latino-américaine à travers les textes, en version brésilienne, d’Octavio Paz (O labirinto da solidão, d’Eduardo Subirats (A penúltima visão do paraíso et de l’oeuvre du dramaturge français Michel Azama (Aztèques.Mots-clés: Littérature comparée; littérature mexicaine; Octavio Paz; Laberinto de la soledad; Eduardo Subirats; Una última visión del paraíso; littérature française; Michel Azama; Aztèques.Keywords: Comparative literature; Mexican literature; Octavio Paz; Laberinto de la soledad; Eduardo Subirats; Una última visión del paraíso; French literature; Michel Azama; Aztèques.

  7. Comportamento de cultivares de alface tipo americana em Boa Esperança Performance of crisphead lettuce cultivars in Boa Esperança, Brazil

    Jony E. Yuri

    2002-06-01

    Full Text Available Os experimentos foram conduzidos em estufas (túnel alto no município de Boa Esperança (MG, para avaliar cultivares de alface do grupo americana ("Crisphead lettuce", em duas épocas de plantio (outubro e março. As cultivares (Cassino; Legacy; Lucy Brown; Lorca; Lady e Raider foram plantadas em delineamento de blocos casualizados com quatro repetições. Para a primeira época (outubro, a produção comercial variou de 29,3 t.ha-1 a 37,6 t.ha-1 e circunferência da cabeça de 40,07 a 42,95 cm, não apresentando diferenças significativas entre cultivares. Na segunda época de plantio (março, sobressaíram-se as cultivares Raider, Cassino, Lady, Legacy e Lorca com produção comercial de 42,6; 41,6; 39,3; 37,3 e 32,5 t.ha-1, respectivamente. Quanto à circunferência de cabeça comercial, as cultivares Lady, Raider, Lucy Brown e Cassino, foram superiores às demais cultivares, com 48,15; 46,69; 45,90 e 45,67 cm respectivamente, sem diferirem significativamente entre si.The experiment was carried out at green house, in Boa Esperança, Brazil, to evaluate lettuce cultivars (Cassino; Legacy; Lucy Brown; Lorca; Lady and Raider, crisphead group, in two planting seasons (October and March. The experimental design was of randomized blocks with four replications. For the first season (October, the commercial yield, varied from 29.3 t.ha-1 to 37.6 t.ha-1 and head circumference from 40.07 to 42.95 cm, without significant difference among cultivars. At the second planting season (March, cvs. Raider, Cassino, Lady, Legacy and Lorca presented a commercial yield of 42.6; 41.6; 39.3; 37.3 and 32.5 t.ha-1. Cvs. Lady, Raider Lucy Brown and Cassino, were superior to the other cultivars, without significant differences among themselves, with 48.15; 46.69; 45.90; and 45.67 cm respectively.

  8. Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: I. investigação epidemiolôgica clínica e laboratorial

    Nelson A. Araújo Filho

    1981-12-01

    Full Text Available Os autores descrevem pela primeira vez a ocorrência de uma epidemia de Leishmaniose Tegumentar Americana (LTAI na localidade de Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, situada a 17 Km do continente. Em uma população de 453 pessoas examinadas, foram encontrados 33 casos de LTA, sendo 13 lesões em atividade e 20 cicatrizes. Entre os 33 casos, 30 eram autóctones da área de estudo. A doença ocorria há mais de 10 anos na Praia Vermelha, e, na Ilha Grande, há mais de 30 anos, sob a forma de casos esporádicos. Na Praia Vermelha, no período de 1974 a 1976, ocorreu uma epidemia com 27 casos da doença. A L TA, na Praia Vermelha, assume características de transmissão em ambiente domiciliar, atingindo indivíduos indistintamente, sem relação com sexo, idade ou profissão. Predominou a forma ulcerada, em extremidades do corpo com ocorrência de cura espontânea das lesões e não comprometimento das mucosas; um hamster entre 10 inoculados com material de lesão de caso humano desenvolveu, após 4 meses, lesão de focinho com resultados positivos para LTA.The authors described for the first time the appearance of an epidemic of Cutaneous Leishmaniasis in the small village of Praia Vermelha, Ilha Grande (Creat Island, county of Angra dos Reis, State of Rio de Janeiro, situated at 17 Km from the continent. 453 persons were examined and 33 cases of Cutaneous Leishmaniasis were found of which 13 were cases of active iesions and 20 of scars. Of the 33 cases, 30 were issued from the area under examination. The disease has existed in Praia Vermelha for more than 10 years and in the Ilha Grande for more than 30 as sporadic cases. In Praia Vermelha between 1974 and 1976, an epidemic occured when 27 cases of the disease were registered. The Cutaneous Leishmaniasis in Praia Vermelha has characteristics of home transmission affecting people without discrimination of sex, age or occupation. The ulcerated form appeared

  9. Phytolacca americana from contaminated and noncontaminated soils of South Korea: Effects of elevated temperature, CO2 and simulated acid rain on plant growth response

    Kim, Y.-O.; Rodriguez, R.J.; Lee, E.J.; Redman, R.S.

    2008-01-01

    Chemical analyses performed on the invasive weed Phytolacca americana (pokeweed) growing in industrially contaminated (Ulsan) and noncontaminated (Suwon) sites in South Korea indicated that the levels of phenolic compounds and various elements that include some heavy metals (Al, As, B, Cd, Co, Cu, Fe, Mn, Ni, Pb, and Zn) were statistically higher in Ulsan soils compared to Suwon soils with Al being the highest (>1,116 mg/l compared to 432 mg/l). Analysis of metals and nutrients (K, Na, Ca, Mg, Cl, NH4, N, P, S) in plant tissues indicated that accumulation occurred dominantly in plant leaves with Al levels being 33.8 times higher in Ulsan plants (PaU) compared to Suwon plants (PaS). The ability of PaU and PaS to tolerate stress was evaluated under controlled conditions by varying atmospheric CO2 and temperature and soil pH. When grown in pH 6.4 soils, the highest growth rate of PaU and PaS plants occurred at elevated (30??C) and non-elevated (25??C) temperatures, respectively. Both PaU and PaS plants showed the highest and lowest growth rates when exposed to atmospheric CO2 levels of 360 and 650 ppm, respectively. The impact of soil pH (2-6.4) on seed germination rates, plant growth, chlorophyll content, and the accumulation of phenolics were measured to assess the effects of industrial pollution and global-warming-related stresses on plants. The highest seed germination rate and chlorophyll content occurred at pH 2.0 for both PaU and PaS plants. Increased pH from 2-5 correlated to increased phenolic compounds and decreased chlorophyll content. However, at pH 6.4, a marked decrease in phenolic compounds, was observed and chlorophyll content increased. These results suggest that although plants from Ulsan and Suwon sites are the same species, they differ in the ability to deal with various stresses. ?? 2008 Springer Science+Business Media, LLC.

  10. 鳄梨组织培养繁殖体系优化研究%Regeneration system establishment of Persea americana

    何碧珠; 吴沙沙; 兰思仁

    2016-01-01

    To meet the need of Persea americana tissue culture, biotechnology was used to provide substantial good quality tissue culture seedlings, which is significant for the industry development and applic ation. Taking stem segments of mutant strains of P. americana as explants, optimum medium for sterile strains establishment, latent bud germination, bud multiplication, and rooting were selected. The results revealed that:the optimum medium of sterile strains establishment was WPM+1.0 mg•L-16 ̄BA+0.3 mg•L-1 NAA+0.5 mg•L-1ZT+2% sugar+0.2% activated carbon+0.65% agar; the optimum medium of latent bud germination was WPM+2.0 mg•L-16 ̄BA+0.5 mg•L-1NAA+1.0 mg•L-1ZT+2% sugar+0.2% activated carbon+0.65% agar, with a bud induction rate to 54%; the optimum medium of bud multiplication was WPM+3.0 mg•L-16 ̄BA+0.3 mg•L-1NAA+1.0 mg•L-1ZT+2% sugar+0.2%activated carbon+0.65% agar, with a reproduction rate up to 3.70;the optimum medium of root culture was 1/2 WPM+0.5 mg•L-1 IBA+0.5 mg•L-1 NAA+2% sugar+0.2% activated carbon+0.65% agar, with a rooting rate of 90%. The survival rate was 96% after transplanted for 60 d. The culture formula in the research can produce complete plantlet and gain lots of high quality seedlings in a short term.%为满足市场需求,以鳄梨饱满茎段为外植体采用组织培养方法进行优质种苗繁育,对于该种的产业发展和应有具有重要意义。试验筛选了无菌株体系建立、诱导潜伏芽萌发、丛生芽继代增殖和生根培养较适培养基配方。结果表明:诱导鳄梨无菌株系建立较适培养基为WPM+1.0 mg•L-16 ̄BA+0.3 mg•L-1NAA+0.5 mg•L-1ZT+2%蔗糖+0.2%活性炭+0.65%琼脂;诱导潜伏芽萌发较适培养基为WPM+2.0mg•L-16 ̄BA+0.5mg•L-1NAA+1.0 mg•L-1ZT+2%蔗糖+0.2%活性炭+0.65%琼脂,诱导出芽率达到54%;丛生芽继代增殖培养较适培养基为WPM+3.0 mg•L-16 ̄BA+0.3 mg•L-1

  11. Shyness and boldness in greater rheas Rhea americana Linnaeus (Rheiformes, Rheidae: the effects of antipredator training on the personality of the birds Timidez e coragem em emas Rhea americana Linnaeus (Rheiformes, Rheidae: os efeitos do treinamento anti-predação na personalidade das aves

    Cristiano S. de Azevedo

    2006-03-01

    Full Text Available The shy-bold continuum is an axis of behavioural variation for some species, but the consequences of shyness and boldness in antipredatory behaviour is unknown. Bold animals have the tendency to be predated first after release in comparison to shy animals, who naturally avoid the predators. Antipredatory training has been used to enhance the defence behaviours of naive animals by various researchers around the world. For greater rheas, Rhea americana (Linnaeus, 1758, this kind of study is pioneer. In this study we have investigated if there are relationships between personality and performance of greater rheas in antipredatory training. We also investigated if the training procedures influenced the behaviour of the birds when presented to novel objects. Fifteen zoo-borne greater rheas were studied and 16 personality tests were run, being eight before the application of antipredator training, and eight after the training. We presented to the birds four novel objects (ball, box, bag and person and recorded their behaviour and the distance of the birds in relation to the objects. Results showed that the birds behave boldly before training and shyly after it. The antipredator training modified significantly the behaviour of the rheas, making them more careful about novel situations. Personalities affected the behaviour of the birds during antipredator training. The study of the animal personalities can be an useful tool in reintroduction programs since it helps to choose the animals with the highest chance of survival to reintroduce.O contínuo timidez-coragem é um eixo de variação comportamental para algumas espécies, mas as conseqüências da timidez e coragem no comportamento anti-predação não são conhecidas. Animais corajosos tendem a ser predados primeiramente após a reintrodução em comparação com os animais tímidos, que naturalmente evitam os predadores. O treinamento anti-predação tem sido usado para aumentar os comportamentos

  12. Casos de leishmaniose tegumentar americana por Leishmania (viannia braziliensis nos municípios de cosmópolis e Indaiatuba - região de Campinas, estado de São Paulo, Brasil

    Selma Giorgio

    1996-10-01

    Full Text Available Com o objetiiro de identificar a espécie de Leishmania envolvida nas afecções cutâneas de indivíduos residentes nos municípios de Cosmópolis e Indaiatuba, região de Campinas, Estado de São Paulo, correlacionamos dados clínicos, histopatológicos e testes de bibridização in situ. Os expressivos índices de incidência de leishmaniose tegumentar americana obsewados nesses municípios nos levaram a iniciar esses estudos. Nesse trabalho, apresentamos os dados relativos a seis indivíduos. As características das lesões, ulceradas, de dificil cicatrização e presentes em locais expostos do corpo sugeriram quadros de leishmaniose tegumentar americana. Os testes de intradermoreação de Montenegro, positivos em cinco dos seis pacientes analisados também reforçaram a suspeita de leishmaniose tegumentar americana. Os padrões histopatológicos, como reação exsudativa celular e granulomatosa e a dificuldade de isolamento dos parasitas obtidos de biópsias de lesões são compatíveis com aqueles descritos para Leishmania (Viannia braziliensis. Em testes de bibridização in situ do DNA do cinetoplasto de amastigotas das lesões dos seis pacientes, obseivamos que quatro deles apresentavam sinais de bibridização com a sonda de L. (Viannia braziliensis, confirmando as suspeitas de que a Leishmania responsável pelas afecções cutâneas nos pacientes analisados era do subgenêro Viannia e do complexo braziliensis.A study was earned out to identify Leishmania species involved in skin lesions of patients from Cosmõpolis and Indaiatuba, State of São Paulo, Brazil. The epidemiological data of cutaneous leishmaniasis in two cities suggested a epidemic situation in 1994. The lesions were clinically characteristic of cutaneous leishmaniasis and five out six patients responded positively to Montenegro's intradermal test. The histopathology of skin lesions were characterized by two patterns: exsudative- cellular reaction and exsudative

  13. A conexão americana: Mattoso Câmara e o círculo lingüístico de Nova Iorque The American connection: Mattoso Câmara and the New York linguistic circle

    Cristina Altman

    2004-01-01

    Full Text Available Quando se pensa nas conexões entre as lingüísticas brasileira e norte-americana, a primeira referência que nos vem à mente é o Círculo Lingüístico de Nova Iorque, onde Roman Jakobson (1896-1982 e Claude Lévi-Strauss (n. 1908 - recém chegado do Brasil, onde permanecera entre 1935 e 1939 - se encontraram e discutiram suas idéias. Este encontro excepcional obscureceu outros contatos entre a lingüística brasileira e a norte-americana, entre eles, aquele ocorrido entre Jakobson e o lingüista brasileiro Joaquim Mattoso Câmara Jr. (1904-1980, que teve conseqüências bem maiores, ao menos no que diz respeito à implementação da lingüística estrutural no Brasil e na América do Sul nos anos 50 e 60. O impacto de Jakobson e da lingüística norte-americana no trabalho de Mattoso Câmara e sua subseqüente influência no desenvolvimento da lingüística brasileira são o foco do presente artigo.When mention is made of Brazil in connection with American linguistics, it usually amounts to a reference to the Linguistic Circle of New York, where Roman Jakobson (1896-1982 and Claude Lévi-Strauss (b. 1908 - who had recently come from Brazil where he stayed from 1935 until 1939 - met and exchanged ideas. This singular event has cast a shadow on other contacts between Brazil and American linguistics, among which the one between Jakobson and the Brazilian linguist Joaquim Mattoso Câmara (1904-1970 was much more consequential, at least in what concerns the implementation of structural linguistics in Brazil and in South America during the 1950s and the 1960s. The impact of Jakobson and of American Linguistics on the work of Mattoso Câmara and its subsequent influence on developments of Brazilian linguistics are the focus of the article.

  14. The Bridges of Americana County

    Christiansen, Steen Ledet

    2011-01-01

    Siden Mystic River har Clint Eastwood cementeret sin status som Hollywood kæledægge og ikon som filmskaber. Inden da, var Clint Eastwood dog et ikon som skuespiller primært for sine roller i Sergio Leones westerns, samt som Harry Callahan i Dirty Harry filmene. Det er netop som ikon, at Clint Eas...

  15. Histopathology of human American cutaneous leishmaniasis before and after treatment Histopatologia de leishmaniose tegumentar americana humana, antes e após o tratamento

    A.C.C. Botelho

    1998-02-01

    cicatriz poderá ser de grande valia. Este trabalho propõe caracterizar o padrão histopatológico de casos humanos de leishmaniose tegumentar americana, em 32 pacientes do município de Caratinga-MG, antes e após o tratamento com os seguintes métodos terapêuticos: 1 leishvacin + glucantime; 2 leishvacin + BCG associado ao glucantime; 3 glucantime; 4 leishvacin + BCG. Foram colhidos fragmentos das lesões de todos os pacientes, através de biópsias, antes e após o tratamento, com diagnóstico de cura. Após análise das lâminas, as preparações foram descritas, do ponto de vista histopatológico, e agrupadas levando em conta a prevalência e a significância do elemento característico. Tal processo resultou na classificação: 1. reação exsudativa; 2. reação exsudativa giganto-celular; 3. reação exsudativa produtiva; 4. reação exsudativa produtiva giganto-celular; 5. reação exsudativa produtiva necrótica; 6. reação necrótica exsudativa; 7. reação produtiva exsudativa; 8. reação produtiva giganto-celular; 9. reação produtiva exsudativa giganto-celular; 10. reação produtiva exsudativa giganto-celular granulomatosa; 11. reação produtiva e 12. reação produtiva cicatricial (cura histopatológica. Observamos após tal análise, que nem sempre a cura clínica coincide com a cura histopatológica.

  16. Leishmaniose tegumentar americana: histórico, epidemiologia e perspectivas de controle American cutaneous leishmaniasis: history, epidemiology and prospects for control

    Sergio de Almeida Basano

    2004-09-01

    Full Text Available A Leishmaniose Tegumentar Americana (LTA é uma doença causada por protozoários do gênero Leishmania, transmitida ao homem pela picada de mosquitos flebotomíneos (Ordem Diptera; Família Psychodidae; Sub-Família Phlebotominae. No Brasil existem atualmente 6 espécies de Leishmania responsáveis pela doença humana, e mais de 200 espécies de flebotomíneos implicados em sua transmissão. Trata-se de uma doença que acompanha o homem desde tempos remotos e que tem apresentado, nos últimos 20 anos, um aumento do número de casos e ampliação de sua ocorrência geográfica, sendo encontrada atualmente em todos os Estados brasileiros, sob diferentes perfis epidemiológicos. Estima-se que, entre 1985 e 2003, ocorreram 523.975 casos autóctones, a sua maior parte nas regiões Nordeste e Norte do Brasil. Neste estudo, são discutidos aspectos relacionados ao tratamento e ao controle dessa doença, assim como também as dificuldades para a implementação dessas medidas. São apontadas alternativas que passam pela estruturação dos serviços de saúde, com respeito ao diagnóstico, no desenvolvimento de drogas de aplicação tópica ou por via oral, no desenvolvimento de vacinas, no controle diferenciado de vetores e no aprofundamento de estudos relacionados à biologia celular do parasita.American Cutaneous Leishmaniasis (ACL is an infectious disease transmitted by the bite of phlebotomines mosquitos (Order Diptera: Family Psychodidae: Sub-Family Phlebotominae and caused by protozoa from the genus Leishmania (ROSS 1903. In Brazil, there are six different species of Leishmania and more than 200 different species of phlebotomines. It's a disease that has been afflicting human beings for many centuries, and in Brazil, in the past two decades, there has been an important increase in the number of cases and also in its geographical distribution. Presently, ACL cases are registered in all Brazilian states under three different epidemiological

  17. Comportamento de cultivares de alface americana em Santo Antônio do Amparo Evaluation of crisphead lettuce cultivars in Santo Antônio do Amparo, Brazil

    Jony E. Yuri

    2005-12-01

    Full Text Available Com o objetivo de avaliar o comportamento de cultivares de alface do tipo americana em duas épocas de cultivo, foram conduzidos dois experimentos em Santo Antônio do Amparo (MG, de setembro a dezembro de 1998 e fevereiro a maio de 1999 em condições de túnel. Utilizou-se o delineamento de blocos ao acaso com seis cultivares (Cassino; Legacy; Lucy Brown; Lorca; Lady e Raider e quatro repetições. As avaliações de massa fresca total e comercial, circunferência e comprimento do caule da cabeça comercial foram realizadas quando as plantas apresentaram cabeça bem formada e compacta. Na primeira época de plantio, sobressaíram-se na característica massa fresca total as cultivares Lady (820,4 g planta-1 e Lucy Brown (790,7 g planta-1 sem, contudo, diferirem da cultivar Lorca (626,6 g planta-1. Para massa fresca comercial destacaram-se as cultivares Lady (620,0 g planta-1 e Lucy Brown (559,3 g planta-1 com maiores rendimentos. A circunferência da cabeça comercial variou de 36,3 a 47,2 cm e o comprimento do caule de 3,3 a 4,5 cm. Para a segunda época de cultivo não observou-se diferenças significativas entre as cultivares para massa fresca total e comercial. No entanto, todas as cultivares apresentaram massa fresca comercial acima de 850 g planta-1. A circunferência da cabeça variou de 45,4 a 53,4 cm e o comprimento de caule de 3,7 a 4,9 cm. Maiores rendimentos em termos de massa fresca foram obtidos na segunda época de plantio.The behavior of crisphead lettuce cultivars at two planting periods was evaluated in two experiments carried in Santo Antônio do Amparo, Minas Gerais State, Brazil, from September to December 1998 and February to May 1999, under a plastic tunnel conditions. The experimental design was a randomized complete block with six treatments (cv Cassino; Legacy; Lucy Brown; Lorca; Lady and Raider with four replications. The evaluations of total and marketable fresh weight, circumference and stem length of the marketable

  18. Epidemiologia e quadro clínico do acidente por cascavel sul-americana (Crotalus durissus Epidemiology and clinical features of South American rattlesnakes (Crotalus durissus envenomation

    Miguel Tanús Jorge

    1992-08-01

    Full Text Available Foram estudados 249 casos de acidentes por cascavel sul-americana (Crotalus durissus atendidos no HVB-IB, São Paulo, Brasil, de 1974 a 1990. Os acidentes foram mais comuns no período da tarde, nos meses de janeiro a abril e de outubro a dezembro. Dentre cem serpentes classificadas quanto à subespécie 99 eram C. d. terrificus. Pertenciam ao sexo masculino 80,7% dos pacientes. Os membros inferiores e superiores foram picados em, respectivamente, 66,4% e 29,2% dos casos. As manifestações clínicas mais freqüentes foram dor (61,0% e edema (55,0% no local da picada, ptose palpebral (75,9%, escurecimento da urina atribuível à mioglobinúria (38,6% e mialgia (36,1%. Nove pacientes foram submetidos a diálise devido a insuficiência renal aguda (3,6%, três apresentavam insuficiência respiratória que motivou intubação e/ou traqueostomia e um apresentou acidente vascular cerebral isquêmico. A alteração da coagulação sanguínea ocorreu em 48,1% dos pacientes. Oito amostras de sangue colhidas horas após a picada mostraram leucocitose sendo 6 com desvio a esquerda e, nos dias subseqüentes, tendência à normalização do número de leucócitos e aparecimento de eosinofilia. Atividade sérica da creatinoquinase apresentou-se aumentada em 20 dentre 21 pacientes, sendo maior no final das primeiras 24 horas após a picada, chegando a 2.377 vezes o valor de referência. A letalidade foi de 0,8%.Under study were two hundred and forty nine cases of accidents caused by South American rattlesnakes (Crotalus durissus admitted to the Vital Brasil Hospital, São Paulo, Brasil, from 1974 to 1990. The accidents were more common in the afternoon, from January to April and from October to December. Ninety nine out of 100 snakes classified as subspecies were C. d. terrificus. Most of the patients were males (80.7%. The inferior and superior limbs were bitten in 66.4% and 29.2% of the cases, respectively. The more frequent clinical manifestations were

  19. Active packaging for fresh-cut iceberg lettuce (Lactuca sativa L. / Embalagem ativa para alface americana (Lactuca sativa L. minimamente processada

    Fábio Yamashita

    2010-09-01

    Full Text Available The minimal processing of horticultural products endears the raw material and is convenient to the consumer due to the facility for preparing and consuming. The objective of this work was to develop an active packaging for fresh-cut iceberg lettuce (Lactuca sativa L. to increase its shelf life. Minimally processing methodology was defined and the product quality was determined by sensorial, physico-chemical and microbiological analysis. The lettuce tree was selected, trimmed, pre-washed, soaked in sanitized water (100ppm of active chlorine for 15 minutes and centrifuged. The lettuce leafs were packed in polypropylene pots with a sachet containing 1-methylcyclopropene (1-MCP and sealed with biodegradable starch film. Soon after the processing, the product was stored at 4oC for 12 days. Periodically samples were evaluated in terms of appearance and purchase intention, soluble solids and vitamin C contents, pH, texture, color, weight loss and total count of mesophilic and psicotrofic microorganisms, lactic bacteria, mould and yeast. Using 1-MCP in sachet format did not increase the lettuce shelf life but the minimal processing combined with packaging sealed with biodegradable film is practicable as the product showed good sensorial acceptance, low microbiological counts and shelf life of 5 days at 4oC.O processamento mínimo de produtos hortícolas agrega valor à matéria-prima e são convenientes ao consumidor, devido à facilidade de preparo e consumo. O objetivo do trabalho foi desenvolver uma embalagem ativa para alface americana (Lactuca sativa L. minimamente processada visando o aumento da vida útil. Foi definida uma metodologia de processamento mínimo e a qualidade do produto foi acompanhada através de avaliação sensorial, físico-química e microbiológica. As cabeças de alface foram desfolhadas, selecionadas, pré-lavadas, sanificadas (100 ppm de cloro ativo por 15 minutos e centrifugadas. As folhas foram acondicionadas em potes de

  20. Composto homeopático reduz a liberação de ânion superóxido pelas células mononucleares de ema (Rhea americana

    W.R Bertoldo

    2011-10-01

    Full Text Available The action of the "Stress Factor Ostrich (Arenales - Fauna and Flora" was tested in the release of superoxide anion by cells in the peripheral blood of rhea (Rhea americana. Sixteen samples of 0.5mL of venous blood were collected through the jugular vein in the morning and placed in heparinized tubes. The leukocytes were separated into polymorphonuclear (PMN and mononuclear (MN. The production of superoxide anion by phagocytes of peripheral blood was determined using the chromogen ferricytochrome C. There was a reduction of superoxide by MN cells in the presence of "Stress Factor Ostrich" indicating a positive influence of product against oxidative stress. Furthermore, future researches, such as the evaluation of other reactive oxygen intermediates and antioxidant enzymes, researches.

  1. Do GIZ ao AVA: a importância de um centro virtual de ensino de língua e cultura hispano-americana para discentes da rede pública de ensino da cidade de Salvador-Bahia

    Elissandro dos Santos Santana

    2013-01-01

    Este trabalho é fruto de um projeto de intervenção e de lei em torno das contribuições de um Centro Virtual de Ensino Língua e Cultura Hispano-americana para discentes da rede pública de ensino da cidade do Salvador, Bahia. Para tanto, optou-se por uma pesquisa bibliográfica, por meio do levantamento de fontes primárias e secundárias, com vistas à fundamentação necessária para a consubstanciação de um plano de caráter interventivo que culminou em um trabalho de conclusão de especialização em ...

  2. Las libertades de pensamiento y expresión, de asociación y reunión en la Convención Americana de Derechos Humanos y la jurisprudencia de la Corte Interamericana

    Francisco José Eguiguren Praeli

    2012-12-01

    Full Text Available La Convención Americana de Derechos Humanos (o Pacto de San José contem­pla, dentro de los derechos protegidos, a las libertades de pensamiento y expresión (artículo 13, de asociación (artículo 16 y de reunión (artículo 15. La Corte Interamericana de Derechos Humanos ha establecido que estas libertades son una trilogía que se constituye como pilar básico de sustento para la democracia, dado que permiten efectivizar la participación política plural de todos los grupos sociales en defensa de sus intereses1. Por ello ha interpretado que estas libertades, así como los derechos políticos, «son de importancia fundamental dentro del Sistema Interamericano por estar estrechamente interrelacionados para posibilitar, en conjunto, el juego democrático

  3. 垂序商陆幼苗及其过渡区维管系统的解剖学研究%Anatomical Studies on the Vegetative Organs and Transition Vascular System of Phytolacca Americana Seedling

    晏升禄; 崔丽; 徐爽; 李祖任; 胡楠; 廖海民

    2012-01-01

    采用常规石蜡制片手段对垂序商陆幼苗进行形态解剖学研究.结果表明:垂序商陆幼苗类型为木兰型.根的初生结构由表皮、皮层和中柱组成,四原型;茎由表皮、皮层和维管柱构成,维管柱包括维管束、髓和髓射线3部分;子叶叶肉分化不明显,初生叶栅栏组织和海绵组织较为发达;幼苗过渡区维管系统属于“根—下胚轴—子叶”形式,过渡区位于下胚轴基部.%An anatomical study was conducted on the vegetative organ of Phytolacca Americana by the common plant paraffin section. The results showed that the seedling type of Phytolacca Americana was Magnolia Type. The root was diarch, which consisted of epidermis, cortex and stele. The stem consisted of epidermis, cortex and vascular cylinder. The vascular cylinder contained vascular bundle, pith and pith ray. The differentiation of cotyledon mesophyll was not obvious, however, the palisade tissue and the spongy tissue of primary leaf was well-developed. Transition vascular system was"root-hypocotyl-cotyledon" form and the transition zone was formed in the base of the hypocotyl.

  4. Utilização da reação de imunofluorescência indireta no acompanhamento da terapêutica da leishmaniose tegumentar americana'

    Wilson Jacinto Silva de Souza

    1982-09-01

    Full Text Available Foram estudados 17 indivíduos cujas idades variaram de 2 a 78 anos, procedentes da área endêmica de Jacarepaguá (RJ, com diagnóstico clínico, epidemiológico, imunológico e parasitológico de leishmaniose tegumentar americana. Todos foram tratados pelo antimoniato de N-metilglucamina na dose de 60 mg/kg/dia em três séries de 10 dias de duração, com 10 dias de intervalo entre cada série e submetidos à coleta e sangue venoso para a reação de imunofluorescência indireta (IF-IgG antes, durante e após o medicamento. Estes 17 indivíduos foram reatores a intradermorreação de Montenegro (= 5 mm e soro-reagentes (= 1:45 na IF-1gG, sendo que em 6 deles (31,7% foi demonstrado ou isolado Leishmania braziliensis. A positividade da IF-IgG antes do tratamento foi de 76,4% (13 casos com título = 1:90. As médias geométricas das recíprocas dos títulos antes da primeira série do antimonial (89,9, durante o tratamento (63,6 a 29,3 e 10, 30 e 120 dias após medicação, mostraram uma graduação nitidamente decrescente (14,9;2,1 e 1,2, respectivamente. Todos tiveram suas lesões cicatrizadas ao final do tratamento sendo maior o número de cicatrizações após a 2°série. Somente 120 dias após a terapêutica, foram observados títulos abaixo de 1:45 na quase todalidade dos pacientes (16 casos - 94,1% sugerindo que, na utilização da IF-IgG como controle de cura, faz-se necessário um acompanhamento sorológico de no mínimo 3 a 4 meses.Seventeen persons, from 2 to 78 years of age, with clinical, epidemiological,immunological and parasitological diagnosis of american tegumentary leishmaniasis, were studied. All came from the endemic area of Jacarepaguá, a suburb of the city of Rio de Janeiro. The patients were treated with n-methylglucanine antimonate, using a dosage of 60 mg/kg/day, in three series of ten days duration each series, with intervals of ten days between each series, venous blood samples having been taken for the indirect

  5. Molluscicidal effect of Phytolacca americana Linn leaf against Oncomelania hupensis and its acute toxicity%垂序商陆叶灭螺活性及其毒性的初步研究

    房伟伟; 陈钧; 韩邦兴; 李晨光; 杨学娟; 张春晓; 姚虎

    2011-01-01

    Objective To study the molluscicidal activity, the influence on glycogen content of Oncomelania hupensis and the acute toxicity to zebra fish of the extract from Phytolacca americana Linn leaf. Methods The different polar factions of the extract of Phytolacca americana Linn leaf were separated by using the systemic solvent segregation method, and then the molluscicidal activity of the fractions was detected according to the Laboratory Final MiUuscicides Screening Method issued by WHO. The glycogen content of soft tissues of Oncomelania hupensis treated by the ethyl acetate polar fraction was determined by the anthrone method. Finally, the acute toxicity of the ethyl acetate polar fraction to non-targets was studied with zebra fish. Results The ethyl acetate polar fraction was the best active components against the snails. Its 48 h LC? And LC? Were 6.0 mg/100 ml and 26.1 mg/ 100 ml, respectively. The glycogen content of soft tissues of the snails decreased by 20% after treated with the fraction. The fish treated by the concentration of LC? (48 h) of the ethyl acetate polar fraction survived for 12 h. Conclusion The Phytolacca americana linn leaf possesses an adequate molluscicidal activity and a significant acute toxicity to the zebra fish.%目的 探讨垂序商陆叶杀灭钉螺效果、对钉螺糖原的影响及其对斑马鱼的急性毒性.方法 用系统分离法分离垂序商陆叶的各极性部位,采用WHO推荐的"杀螺剂实验室终筛法"中的浸泡法进行浸杀灭螺试验,筛选垂序商陆叶的灭螺活性部位;用蒽酮比色法测定灭螺活性部位对钉螺糖原的影响;测定垂序商陆叶极性部位对斑马鱼的急性毒性.结果 垂序商陆叶灭螺活性部位集中在乙酸乙酯极性部位,对钉螺48 h LC_50和LC_90值分别为6.0 mg/100 ml和26.1 mg/100 ml;垂序商陆叶乙酸乙酯极性部位可使钉螺糖原含量降低20%;在近LC_50浓度(48 h)的垂序商陆叶乙酸乙酯极性部

  6. Comportamento produtivo e econômico da alface americana em função de diferentes lâminas de água Economic and productive behavior of production of crisphead lettuce under different irrigation depths

    Joaquim A. de Lima Júnior

    2011-11-01

    Full Text Available Visando às dificuldades encontradas pelo produtor no cultivo da alface (Lactuta sativa L. americana, cv. Laureau, especificamente quanto à falta de informações técnicas sobre quantidade de água a aplicar, objetivou-se estudar o efeito de diferentes lâminas de água sobre o comportamento produtivo e econômico da alface americana. O experimento foi realizado na Universidade Federal de Lavras, no período de outubro a dezembro de 2008, em casa de vegetação. O delineamento experimental foi em blocos casualizados, com cinco tratamentos e quatro repetições. Os tratamentos foram constituídos de cinco fatores de evaporação, correspondentes a 0,30, 0,60, 0,90, 1,20 e 1,50 EVm, baseados na lâmina evaporada de um minitanque. Os resultados permitiram concluir que a máxima produtividade total e comercial, 65.918 kg ha-1 e 35.544 kg ha-1, foram estimadas com a aplicação de lâminas equivalentes a 152,1 e 155 mm, respectivamente, correspondentes ao fator de reposição de 92%; considerando o preço do fator água (R$ 0,67 mm-1 e o preço da alface americana (R$ 0,90 kg-1, a lâmina economicamente ótima foi 154 mm, identificando que a eficiência técnica do experimento foi significativamente igual à máxima eficiência econômica.With the aim of minimizing the difficulties faced by the producers on cultivating the crisphead lettuce (Lactuta sativa L., specifically those related to the lack of technical information about the quantity of water to be applied, a study was conducted to determine the economic and productive behavior of crisphead lettuce yielding characteristics. The experiment was carried out at the Universidade Federal de Lavras, from October to December 2008 in greenhouse. A randomized block design with five treatments and four repetitions was adopted. The treatments, consisting of the five respective evaporation factors: 0.30, 0.60, 0.90, 1.20 and 1.50 EVm, were done according to an evaporated depth of a reduced pan. The

  7. 美洲大蠊醇提取物的木瓜蛋白酶酶解工艺%Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain

    罗廷顺; 马芳芳; 高孟婷; 吴少辉; 刘光明; 赵昱; 张成桂

    2012-01-01

    目的:优选木瓜蛋白酶对美洲大蠊醇提取物的酶解工艺.方法:以水解度为指标,通过单因素和正交试验,分别考察酶解温度、酶解时间、pH、加酶量、底物浓度对木瓜蛋白酶酶解美洲大蠊醇提取物的影响.结果:木瓜蛋白酶酶解美洲大蠊醇提取物的最佳工艺条件为酶解温度55℃,时间3h,pH 7.0,加酶量8400 U·mg-1,底物浓度0.5%.在此条件下,水解度可达9.87%.酶解后的样品相对分子质量变小,相对分子质量分布变宽.结论:优选方法稳定,操作简便,为进一步研究美洲大蠊提供依据和基础.%Objective: To study on enzymatic technology of ethanol extract from Periplaneta americana by papain and screen out optimum enzymatic technology. Method: Taking degree of hydrolysis as index, effect of enzymatic temperature, enzymatic time, pH, the amount of papain and substrate concentration on papain were investigated by single factor experiment and orthogonal test Result; Optimum enzymatic technology conditions of papain was determined as follows: enzymatic temperature 55 X, , enzymatic time 3 h, pH 7. 0, the amount of papain 8 400 U ?mg ', substrate concentration 0. 5%. Under these conditions, degree of hydrolysis could reach to 9. 87%. Molecular weight of sample were smaller and molecular weight distribution were wider after enzymolysis. Conclusion: This optimized method was stable, easy to operate, and provided basis and foundation to further study of P. americana.

  8. Efeito da salinidade da água de irrigação na produção da alface americana Effect of irrigation water salinity on american lettuce production

    Eliezer Santurbano Gervásio

    2000-04-01

    Full Text Available Com o objetivo de se verificar o efeito de diferentes concentrações de sais da água de irrigação na produção da alface americana e sua acumulação no extrato saturado durante um ciclo de cultivo, instalou-se o presente experimento em casa de vegetação, no Departamento de Engenharia da Universidade Federal de Lavras, MG. Utilizou-se o delineamento inteiramente casualizado, com cinco repetições, sendo testados seis níveis de salinidade de água (condutividade elétrica de 0,18, 1,0, 1,5, 3,0, 4,5 e 6,0 dS m-1. Os resultados obtidos mostraram que o consumo de água pelas plantas, durante o ciclo de produção, diminuiu com níveis crescentes de salinidade da água de irrigação e que o aumento da salinidade proporcionou decréscimos em todas as variáveis vegetativas avaliadas. A máxima produção comercial da alface americana foi obtida quando a condutividade elétrica do extrato saturado atingiu o valor limite de 0,2 dS m-1. Para cada aumento unitário desta condutividade além do limite citado, houve uma redução de 17% na sua produção comercial.With the objective of verifying the effect of different concentrations of salts in the irrigation water on the production of the American lettuce, as well as its accumulation in the saturation extract during a cultivation cycle, one experiment was conducted under greenhouse conditions in the Department of Engineering of the Federal University of Lavras. The statistical design adapted was completely randomized, with 6 levels of water salinity (electric conductivity of 0.18, 1.0, 1.5, 3.0, 4.5 and 6.0 dS m-1 and 5 replications. The results obtained showed that the consumption of water for the plants during the production cycle decreased with increasing levels of salinity of irrigation water, and that the increase of the salinity caused a reduction in appraised vegetative variables. The maximum commercial production of the American lettuce was obtained when the electrical conductivity of

  9. Atividade antioxidante e quantifi cação de compostos bioativos dos frutos de abricó (Mammea americana
    Antioxidant activity and bioactive compounds of the abricó fruits (Mammea americana

    A. C. C. BRAGA

    2010-09-01

    Full Text Available

    O presente trabalho apresenta a atividade antioxidante, a quantifi cação de compostos bioativos (carotenóides totais – CT, polifenóis totais – PT e fl avanóis totais – FT, e a caracterização física e centesimal dos frutos de abricó (Mammea americana. Foram realizadas análises de cor (sistema L*, a* e b*, atividade antioxidante (Oxigen Radical Absorbance Capacity – ORAC – e Trolox Equivalent Antioxidant Capacity – TEAC, de CT (método espectrofotométrico, de PT (método de Folin-Ciocalteu e de FT (método DMACA. Os frutos apresentaram massa de 463g±153g, diâmetros longitudinal e transversal de, respectivamente, 100±12 e 105±10 mm. Os parâmetros de cor foram: 61,20±7,61 (L*, 13,83±2,39 (a* e 45,92±6,65 (b*. Para a composição centesimal (em g/100g de fruto fresco obteve-se: 14,89g±0,19g de sólidos totais, 1,10g±0,06g de lipídeos, 2,02g±0,55g de proteínas e 0,23g±0,02g de resíduo mineral fi xo, 3,47±0,08 de pH e 12±0,01 ºBrix de sólidos solúveis. Os valores ORAC, TEAC, CT, PT e FT foram, respectivamente: 30,97 μMolET/100g (±2,30, 11,82 μMolET/100g (±1,40, 7,55 mg/100g (±0,78, 25,41 mgEAG/100g (±2,30 e 2,61 mgEC/100g (±0,73. Os resultados das análises físicas evidenciam um fruto de grande variabilidade em função do seu tamanho e coloração. Além disso, destaca-se como fonte de pró-vitamina A, apesar de demonstrar reduzida atividade antioxidante e baixo teor de polifenóis e flavanóis totais.

  10. Alguns dados sobre a leishmaniose visceral americana e doença de Chagas no Nordeste Brasileiro (Relatório de uma excursão realizada nos Estados do Ceará, Pernambuco e Baía

    R. Pondé

    1942-01-01

    Full Text Available Antes de nossas pesquisas, nas zonas emque trabalhamos, haviam sido asinalados cinco casos vivos de Leishmaniose Visceral Americana, três no Ceará e dois em Pernambuco. Alem desses, oito tinham sido evidenciados por viscerotomia na Baía, município de Jacobina. Já se tinha conhecimento tambem, de um caso de Doença de Chagas no Ceará. Na confecção do presente trabalho visitamos regiões dos Estados do Ceará, Pernambuco e Baía, onde realizamos rápidos inquéritos epidemiológicos sobre as duas protozooses. No Ceará, região do Cariri, encontramos dois novos casos de Leishmaniose Visceral Americana, cujo diagnóstico foi confirmado pelo exame de esfregaços de polpa esplênica, e cinco de Doença de Chagas, evidenciandos pelos xenodiagnóstico. Foram capturados Flebotomus longipalpis Lutz & Neiva, F. villelai Mangabeira., F. limai Fonseca e F. nordestinus Mangabeira, assim com Panstrongylus megistus (Burm., 13.5% dos quais infectados. Examinamos animais domésticos (cães e gatos e silvestres com resultado negativo. Em Pernambuco, município de Exú, fizemos um inquérito sobre os dois casos de Leishmaniose Visceral Americana anteriormente assinalados, não encontrando outros. foram capturados F. longipalpis Lutz & Neiva, alem de Panstrongylus magistus (Burm. e Triatoma brasiliensis Neiva; nenhum deles estava infectado. Dos animais domésticos foram autopsiados três cães sendo encontrado um com leishmanias no fígado e no baço. O exame de 43 animais silvestres nada revelou. Na Baía, município de Jacobina, encontramos, na zona periférica da cidade, um caso de Leishmaniose visceral Americana e, em outras localidades do município, três outros casos, cujos diagnósticos foram confirmados pelo exame de esfregaços de polpa esplênica; encontramos, também dois casos de Doença de Chagas, evidenciados pelo xenodiagnóstico. Foram capturados F. evandroi Costa Lima, F. lenti Mangabeira e F. longipalpis Lutz & Neiva; Panstrongylus

  11. El lenguaje de las crónicas de Indias: entre la expresión del imperio español y la expresión americana

    Moreno Blanco Juan

    1995-06-01

    Full Text Available

    El presente artículo busca discernir matices de expresión en la Crónica de Indias, a partir de la consideración del autor de éstas como individualidad de conciencia en su época. Tal individualidad se plasma en la continuidad de la expresión de una lengua o en la innovación en la expresión de la misma. Se postula la posibilidad de establecer diferencias entre las diversas crónicas a partir de la mentalidad del autor, que es captada en su aprehensión de la realidad americana, y la del otro, el americano, nuevo en la cosmovisión europea. Tres matices se distinguen: la crónica que expresa una inercia de la mentalidad del autor europeo y reproduce la opacidad en el conocimiento y la expresión del otro; la crónica que da cabida a la expresión del otro - creando un texto que accede a la policulturalidad - y, por último, la crónica que confronta ideas oficiales de España e inaugura un pensamiento crítico y original en la lengua de esa nación.

  12. Manifestações estomatológicas, contagem de linfócitos T-CD4+ e carga viral de crianças brasileiras e norte-americanas infectadas pelo HIV

    GRANDO Liliane Janete

    2002-01-01

    Full Text Available A prevalência de manifestações estomatológicas associadas à infecção pelo HIV foi avaliada em 184 crianças de ambos os sexos, da faixa etária de zero a 13 anos de idade, atendidas nos Ambulatórios de AIDS Pediátrica do Hospital São Lucas (HSL da PUCRS, e do Hospital de Clínicas de Porto Alegre (HCPA da UFRGS, ambos em Porto Alegre, RS, Brasil e no Centro Pediátrico para Doenças Infecciosas do Hospital Universitário e Departamento de Odontopediatria da Faculdade de Odontologia da Universidade Estadual de Nova Iorque (SUNY em Stony Brook, Nova Iorque, EUA, no período de janeiro de 1999 a maio de 2000. A freqüência de crianças brasileiras (72,73% e norte-americanas (53,66% com manifestações estomatológicas associadas à infecção pelo HIV foi estatisticamente significativa, com destaque para linfadenopatia cérvico-facial, aumento de volume das parótidas, candidíase eritematosa, petéquias, gengivite, xerostomia e queilite angular. As crianças com manifestações estomatológicas apresentaram contagens médias de linfócitos T-CD4+ próximas à normalidade e carga viral alta.

  13. Impacto de la aplicación de los criterios de la asociación americana de tiroides en el diagnóstico de hipotiroidismo en gestantes de Vigo, España

    Marta Pombar-Pérez

    2013-07-01

    Full Text Available Con el objetivo de determinar el impacto de la aplicación de los criterios de la Asociación Americana de Tiroides (ATA en el diagnóstico de hipotiroidismo en gestantes del área sanitaria de Vigo en España, se analizó la concentración sérica de tirotropina (TSH, tiroxina libre (T4L y anticuerpos antitiroideos, comparando la frecuencia de gestantes diagnosticadas de hipotiroidismo aplicando los criterios utilizados en el laboratorio de hormonas del Hospital Xeral y los criterios de referencia propuestos por la ATA. La asunción de dichos criterios implicaría un aumento de un 29,6% de pacientes diagnosticadas de hipotiroidismo. La aplicación de los criterios ATA tuvo una repercusión en la medición de la concentración de TSH en gestantes lo que sugiere una evaluación de los valores de referencia de TSH en función de la población y los métodos de diagnóstico locales

  14. Impacto de la aplicación de los criterios de la asociación americana de tiroides en el diagnóstico de hipotiroidismo en gestantes de Vigo, España

    Marta Pombar-Pérez

    Full Text Available Con el objetivo de determinar el impacto de la aplicación de los criterios de la Asociación Americana de Tiroides (ATA en el diagnóstico de hipotiroidismo en gestantes del área sanitaria de Vigo en España, se analizó la concentración sérica de tirotropina (TSH, tiroxina libre (T4L y anticuerpos antitiroideos, comparando la frecuencia de gestantes diagnosticadas de hipotiroidismo aplicando los criterios utilizados en el laboratorio de hormonas del Hospital Xeral y los criterios de referencia propuestos por la ATA. La asunción de dichos criterios implicaría un aumento de un 29,6% de pacientes diagnosticadas de hipotiroidismo. La aplicación de los criterios ATA tuvo una repercusión en la medición de la concentración de TSH en gestantes lo que sugiere una evaluación de los valores de referencia de TSH en función de la población y los métodos de diagnóstico locales

  15. Uma análise crítica da abordagem dos Determinantes Sociais da Saúde a partir da medicina social e saúde coletiva latino-americana

    Elis Borde

    Full Text Available O enfoque dos Determinantes Sociais da Saúde tem tido ampla difusão e foi aparentemente bem acolhido globalmente. Este artigo assume uma visão crítica desse enfoque, buscando sistematizar as críticas principalmente provenientes de debates no interior da medicina social e saúde coletiva latino-americana, que se articularam ao redor da diferenciação entre Determinantes Sociais da Saúde e a determinação social dos processos saúde-doença. Pretendemos examinar estas diferenças para problematizar a aparente unanimidade retórica em prol da equidade. Ainda que a abordagem dos Determinantes Sociais da Saúde marque um enorme avanço na mobilização pela equidade em saúde, em nossa avaliação ela não consegue ser mais do que um avanço incompleto.

  16. Do GIZ ao AVA: a importância de um centro virtual de ensino de língua e cultura hispano-americana para discentes da rede pública de ensino da cidade de Salvador-Bahia

    Elissandro dos Santos Santana

    2013-05-01

    Full Text Available Este trabalho é fruto de um projeto de intervenção e de lei em torno das contribuições de um Centro Virtual de Ensino Língua e Cultura Hispano-americana para discentes da rede pública de ensino da cidade do Salvador, Bahia. Para tanto, optou-se por uma pesquisa bibliográfica, por meio do levantamento de fontes primárias e secundárias, com vistas à fundamentação necessária para a consubstanciação de um plano de caráter interventivo que culminou em um trabalho de conclusão de especialização em Metodologia do ensino de língua espanhola. Ao longo do texto são abordados os seguintes tópicos: o contexto histórico-social e leis no tangente ao ensino de língua estrangeira no Brasil, a importância do turismo e qualificação de profissionais em língua espanhola para a cidade do Salvador e as possíveis contribuições de um Centro Virtual de ensino de espanhol.

  17. Reiterando o pacto: história, teologias políticas cristãs e a religião civil americana em uma era de multiculturalismo e império

    Bruno Reinhardt

    2011-01-01

    Full Text Available Há 40 anos, em plena Guerra Fria, Robert Bellah concluiu seu célebre artigo sobre a religião civil americana com a seguinte pergunta: como conceber a aplicabilidade legítima dos valores-chave que suportam o imaginário nacional americano para além dos confins territoriais do seu Estado-nação? O presente artigo quer revisitar esta questão criticamente à luz de incursões regulatórias recentes dos EUA sobre o campo da liberdade religiosa no globo. Primeiramente, reviso a história da relação entre secularismo, identidade nacional e cristianismo nos EUA. A seguir, analiso as articulações político-religiosas que dão origem ao International Freedom of Religion Act (IRFA, em 1998. Por fim, tento destacar como este exercício pode fornecer contribuições mais gerais para o estudo da relação entre religião, nacionalismo e poder secular na contemporaeidade, com ênfase na relação entre estes e o princípio de soberania do estado de direito.

  18. Enfoques conceptuales y propuestas metodológicas para el estudio de las interacciones entre el medio ambiente y la salud: aplicación a un programa de investigación sobre la tripanosomiasis americana

    Romaña Cristina

    2003-01-01

    Full Text Available Las modificaciones del paisaje causadas por las actividades y migraciones humanas contribuyen en hacer emerger o re-emerger enfermedades tropicales, particularmente aquellas cuya transmisión se realiza por insectos vectores que a menudo deben escapar a las condiciones cambiantes ambientales, adaptándose y modificando sus redes tróficas, su morfología, hasta su genotipo. Obtener una mejor comprensión de las relaciones entre los factores ecológicos y humanos y las antropozoonosis es vital para poder identificar variables que permitan cartografiar el riesgo para las poblaciones humanas. Este es el objetivo del programa de investigación Ecología del Paisaje, Dinámica de los Agro-Ecosistemas y Complejos Eco-Patógenos: La Definición del Riesgo Eco-Epidemiológico en la Tripanosomiasis Americana que se desarrolla en diferentes ecosistemas del Brasil. Los autores describen las bases conceptuales y metodológicas del programa e insisten sobre el rol del método eco-epidemiológico para el estudio de la estructura y el funcionamiento de los focos naturales y antropizados de la infección. Modelizar su dinámica espacial y temporal permite concebir nuevos útiles de predicción y de vigilancia.

  19. Investigation of the Reasons for the Unique Growth and Development of Agave Species (Agave sisalana and Agave americana Crop Plants at the Southern, Central, North Western and Eastern Parts of Tigray, Ethiopia

    Kinfemichael Geressu Asfaw

    2011-07-01

    Full Text Available Composite topsoil samples from 16 study sites were taken and then their physical as well as chemical attributes were determined at the National Soil Testing Center (NSTC. Moreover, plant attributes such as Leaf Length (LL, Leaf Biomass (LB, Fiber Length (FL and Tensile Strength (TS were measured. Data analysis was carried out using JMP5 (version 5.0 and SPSS (version 12.0 statistical softwares. The one way analysis of variance (ANOVA for Leaf Length (LL, Leaf Biomass (LB, and Tensile Strength (TS showed statistical significance with respect to the study sites ( p0.05 with respect of blocks. On the other hand, the one way ANOVA for both soil chemical and physical attributes with respect to blocks found statistically insignificant (p>0.05. The rainfall, sunshine, and temperature situations as well as the soil chemical and physical attributes being inline with the requirements of Agave species could be reasons for the unique growth and development of A. americana and A. sisalana crop plants in the study area.

  20. Leishmaniose tegumentar americana em centro de treinamento militar localizado na Zona da Mata de Pernambuco, Brasil American cutaneous leishmaniasis in military training unit localized in Zona da Mata of Pernambuco State, Brazil

    Sinval P. Brandão-Filho

    1998-12-01

    Full Text Available Um surto de leishmaniose tegumentar americana ocorreu em 1996 em unidade de treinamento militar situada na Zona da Mata de Pernambuco, com o registro de 26 casos humanos. Um inquérito epidemiológico foi realizado através da realização de levantamento entomológico e da aplicação do Teste de Montenegro. Lutzomyia choti apresentou predominância de 89,9% dos flebótomos identificados. De 545 homens que participaram de treinamentos no período, 24,1% (incluindo os casos clínicos foram positivo para o Teste de Montenegro.An outbreak of American cutaneous leishmaniasis has been occurred in military training unit localized in 'Zona da Mata' of Pernambuco State, Brazil, where were registered 26 human cases. An epidemiological survey was carried out by entomological investigation and Montenegro skin test (MST. Lutzomyia choti presents predominance (89.9% in sandflies identified. Out of 545 men who realized training activities were 24.12% positive to MST.