WorldWideScience

Sample records for aaf

  1. An Analysis of AAFES and Its Relevance to the Future of the Army and Air Force

    Science.gov (United States)

    2009-06-12

    ecommerce activities. The Main Exchange store is typically referred to as the Post Exchange (PX) on Army installations or Base Exchange (BX) on Air...are funded entirely by APF. 70 AAFES operates an extensive catalog sales and ecommerce activity. Authorized AAFES patrons are able to purchase a...AAFES ecommerce and catalog activities are very competitive against similar competing companies, and include an auction site and ship to store feature

  2. Fort Campbell AAF, Clarksville, Kentucky. Revised Uniform Summary of Surface Weather Observations (RUSSWO)

    Science.gov (United States)

    1975-07-10

    FROM HOURLY OBSERVATIONS) 13806 FO~RT CA;*,PSFLL KY/ CAMPRELL AAF 43-45,t5-72 AUG STATION STATIONM ARC ?"Its MONTH (ALL V4,FATKER 1200-1400 CLASS... CAMPRELL AAF 44-45P50-72 APR STATKO, STATIO’ N E Y. "s ONT. PERCENTAGE FREQUENCY OF OCCURRENCE 12o-o (FROM HOURLY OBSERVATIONS) VISIBLITY (STATUTE MILES...T.,S 0EM Ate OM.CITE ( UDATA PRUCESS, b,"ACCh USAF ETAC CEILING VERSUS VISIBILITY AIR WEATHER SERVICE/MAC + 13806 FORT CAMPBELL KY/ CAMPRELL AAF 4"A

  3. Pharmacological and biomolecular investigations of a polyherbal formulation (AAF-6 for its antiasthmatic activity

    Directory of Open Access Journals (Sweden)

    Antony A

    2010-01-01

    Full Text Available We investigated the antiasthmatic activity of a herbal preparation from a combination of six Indian medicinal plants. The current study was undertaken to assess the antiasthmatic activity of a polyherbal formulation (AAF-6 using ovalbumin-induced asthmatic rats. The animals were divided into five groups: solvent control, ova sensitised control, ova sensitised plus Ketotifen (KT, ova sensitised + parallel treatment with AAF-6 and ova sensitised + post-treatment with AAF-6. Total and differential white blood cell count, total protein content, nitrite level, myeloperoxidase (MPO activity and mast cell stabilising activity were estimated. Intragastric administration of AAF-6 (100 mg/kg body weight significantly reduced total and differential white blood cell count, normalised the increased protein level, and decreased the nitrite level and MPO activity, and increased mast cell stabilising activity, when compared with ova sensitised control. Statistical analyses were done by one-way analysis of variance (ANOVA followed by Bonferroni post hoc test at a level of significance P<0.05. AAF-6 exhibited quite a competitive potential when compared with the standard drug KT, affording a possible alternative therapeutic agent in the treatment of asthma.

  4. Immunomodulatory effects of amino acid-based formulae (AAF) in gastrointestinal non-IgE mediated food allergy

    NARCIS (Netherlands)

    Hartog, Anita; Jones, Hannah E.; Harthoorn, Lucien F.; Garssen, Johan; Stephenson, Holly; Brunner, Katja; Köglmeier, Jutta; Shah, Neil; Bajaj-Elliot, Mona; Lindley, Keith J.

    2015-01-01

    RATIONALE: Management of non-IgE mediated food allergies in early childhood involves allergen avoidance by using extensively hydrolysed (eHF) or amino acid-based formulas (AAF). Clinical anecdote suggests that AAF relieve symptoms more effectively in some patients than using eHF or simple allergen a

  5. ENVIRONMENTAL TECHNOLOGY VERIFICATON: TEST REPORT OF CONTROL OF BIOAEROSOLS IN HVAC SYSTEMS AAF INTERNATIONAL DRIPAK 90/95%

    Science.gov (United States)

    The Environmental Technology Verification report discusses the technology and performance of the DriPak 90/95% air filter for dust and bioaerosol filtration manufactured by AAF International. The pressure drop across the filter was 104 Pa clean and 348 Pa dust loaded, and the fil...

  6. ENVIRONMENTAL TECHNOLOGY VERIFICATIONTEST REPORT OF CONTROL OF BIOAEROSOLS IN HVAC SYSTEMS AAF INTERNATIONAL BIOCEL I (TYPE SH)

    Science.gov (United States)

    The Environmental Technology Verification report discusses the technology and performance of the BioCel I (Type SH) air filter for dust and bioaerosol filtration manufactured by AAF International. The pressure drop across the filter was 236 Pa clean and 478 Pa dust loaded, and th...

  7. Bad Tolz AAF, Bad Tolz, Germany. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F

    Science.gov (United States)

    1972-01-26

    PERCENTAGE FREQUENCY OF WIND DIRECTION AND SPEED (FROM HOURLY OBSERVATIONS) 417.. BADL 01-2 OER MANY AAF ---67 STATION STATION kANS VI.$ No 1. ALL WEATHER 21...and aruual for all years Tcc , ... ase pab !atior.s rovide the caunalative percentae fre..ency to tenths of temperature by 5-degrea ’ahrcnheit

  8. Earth resources programs at the Langley Research Center. Part 1: Advanced Applications Flight Experiments (AAFE) and microwave remote sensing program

    Science.gov (United States)

    Parker, R. N.

    1972-01-01

    The earth resources activity is comprised of two basic programs as follows: advanced applications flight experiments, and microwave remote sensing. The two programs are in various stages of implementation, extending from experimental investigations within both the AAFE program and the microwave remote sensing program, to multidisciplinary studies and planning. The purpose of this paper is simply to identify the main thrust of the Langley Research Center activity in earth resources.

  9. FT Stewart AAF, Savannah, Georgia. Revised Uniform Summary of Surface Weather Observations (RUSSWO)

    Science.gov (United States)

    1979-04-18

    HOURLY OBSERVATIONS) 03875 FT STEYART AAF GA _______ 68-70,73-74,76 FEB S T A T IO N S T A T IO N A M [ EA Mt N N T H C’ ALL WIaTfIER 030-0500 CLAN...LI.T.) (A CONDITION SPEED I EA (KNTS) 1 .3 4.6 7. 1 11 16 17 21 22. 27 28-33 34-40 41 .47 48-55 -56 % WIND DIR. SPEED N 1.7 2.4 1.1 5.2 4.* NNE 1.2 1.9...90951 92t0 92,11 92:1 92:11 92:1 92:, 92:21 922 922 92,2 922 92:21.£3000 .68,2 8 .7 90,2.91.6 93.1 9392 93 2 93 2( 93 2 93,2 93 3 93s3 9313 93,.3 9.C3

  10. Deployment of ARM Aerial Facility (AAF) Scanning Mobility Particle Sizer Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Wang, J. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2016-03-31

    Atmospheric aerosols influence global climate by scattering and absorbing sunlight (direct effects) and by changing the microphysical structure, lifetime, and coverage of clouds (indirect effects). While it is widely accepted that aerosol indirect effects act to cool the Earth-atmosphere system by increasing cloud reflectivity and coverage, the magnitudes of the indirect effects are poorly quantified. One key aerosol property for understanding aerosol indirect effects is the ability of aerosol particles to form cloud droplets at atmospheric relevant supersaturations—i.e., cloud condensation nuclei (CCN) activity. For particles consisting of typical atmospheric inorganic compounds, their CCN activity is well understood and can be effectively predicted using Köhler theory based on physicochemical properties of the solute, such as its mass, molar volume, and activity coefficient. However, atmospheric aerosols often consist of hundreds of organic species, which can contribute ~20-90% to the total fine aerosol mass. Depending on their properties, organic species can significantly influence the ability of aerosol particles to act as CCN and form cloud droplets. This project focuses on the CCN activity of secondary organic aerosol compounds formed from key biogenic volatile organic compounds (VOCs) under representative conditions, and the relationship between the hygroscopicity and composition of organic aerosols. The ARM Aerial Facility (AAF) scanning mobility particles sizer (SMPS) was deployed during a ~ 6 week intensive measurement campaign, taking place June 15-July 31 2015 at the Pacific Northwest National Laboratory (PNNL) Environmental Simulation Chamber. The SMPS was operated with a CCN counter. Aerosol particles were first classified by the differential mobility analyzer inside the SMPS; the classified aerosol will then be simultaneously characterized by a condensation particle counter (part of the SMPS) and the CCN counter.

  11. Oval cell response is attenuated by depletion of liver resident macrophages in the 2-AAF/partial hepatectomy rat.

    Directory of Open Access Journals (Sweden)

    Shuai Xiang

    Full Text Available BACKGROUND/AIMS: Macrophages are known to play an important role in hepatocyte mediated liver regeneration by secreting inflammatory mediators. However, there is little information available on the role of resident macrophages in oval cell mediated liver regeneration. In the present study we aimed to investigate the role of macrophages in oval cell expansion induced by 2-acetylaminofluorene/partial hepatectomy (2-AAF/PH in rats. METHODOLOGY/PRINCIPAL FINDINGS: We depleted macrophages in the liver of 2-AAF/PH treated rats by injecting liposome encapsulated clodronate 48 hours before PH. Regeneration of remnant liver mass, as well as proliferation and differentiation of oval cells were measured. We found that macrophage-depleted rats suffered higher mortality and liver transaminase levels. We also showed that depletion of macrophages yielded a significant decrease of EPCAM and PCK positive oval cells in immunohistochemical stained liver sections 9 days after PH. Meanwhile, oval cell differentiation was also attenuated as a result of macrophage depletion, as large foci of small basophilic hepatocytes were observed by day 9 following hepatectomy in control rats whereas they were almost absent in macrophage depleted rats. Accordingly, real-time polymerase chain reaction analysis showed lower expression of albumin mRNA in macrophage depleted livers. Then we assessed whether macrophage depletion may affect hepatic production of stimulating cytokines for liver regeneration. We showed that macrophage-depletion significantly inhibited hepatic expression of tumor necrosis factor-α and interleukin-6, along with a lack of signal transducer and activator of transcription 3 phosphorylation during the early period following hepatectomy. CONCLUSIONS: These data indicate that macrophages play an important role in oval cell mediated liver regeneration in the 2-AAF/PH model.

  12. Participation of liver progenitor cells in liver regeneration: lack of evidence in the AAF/PH rat model.

    Science.gov (United States)

    Dusabineza, Ange-Clarisse; Van Hul, Noémi K; Abarca-Quinones, Jorge; Starkel, Peter; Najimi, Mustapha; Leclercq, Isabelle A

    2012-01-01

    When hepatocyte proliferation is impaired, liver progenitor cells (LPC) are activated to participate in liver regeneration. We used the 2-acetaminofluorene/partial hepatectomy (AAF/PH) model to evaluate the contribution of LPC to liver cell replacement and function restoration. Fischer rats subjected to AAF/PH (or PH alone) were investigated 7, 10 and 14 days post-hepatectomy. Liver mass recovery (LMR) was estimated, and the liver mass to body weight ratio calculated. We used serum albumin and bilirubin levels, and liver albumin mRNA levels to assess the liver function. LPC expansion was analyzed by cytokeratin 19 (CK19), glutathione S-transferase protein (GSTp) immunohistochemistry and by CK19, CD133, transforming growth factor-β1 and hepatocyte growth factor mRNA expression in livers. Cell proliferation was evaluated by Ki67 and BrdU immunostaining. Compared with PH alone where LMR was ∼100% 14 days post-PH, LMR was defective in AAF/PH rats (64.1±15.5%, P=0.0004). LPC expansion was scarce in PH livers (0.5±0.4% of CK19(+) area), but significant in AAF/PH livers (8.5±7.2% of CK19(+)), and inversely correlated to LMR (r(2)=0.63, PPH animals presented liver failure (low serum albumin and high serum bilirubin) 14 days post-PH. Compared with animals with preserved function, this was associated with a lower LMR (50±6.8 vs 74.6±9.4%, P=0.0005), a decreased liver to body weight ratio (2±0.3 vs 3.5±0.6%, P=0.001), and a larger LPC expansion such as proliferating Ki67(+) LPC covered 17.4±4.2% of the liver parenchyma vs 3.1±1.5%, (Plivers with preserved function. These observations suggest that, in this model, the efficient recovery of the liver function was ensured rather by the proliferation of mature hepatocytes than by the LPC expansion and differentiation into hepatocytes.

  13. ENVIRONMENTAL TECHNOLOGY VERIFICATION, TEST REPORT OF CONTROL OF BIOAEROSOLS IN HVAC SYSTEMS:AAF INTERNATIONAL, PERFECTPLEAT ULTRA, 175-102-863

    Science.gov (United States)

    The Environmental Technology Verification report discusses the technology and performance of the PerfectPleat Ultra 175-102-863 air filter for dust and bioaerosol filtration manufactured by AAF International. The pressure drop across the filter was 112 Pa clean and 229 Pa dust lo...

  14. Aggregative adherence fimbriae I (AAF/I) mediate colonization of fresh produce and abiotic surface by Shiga toxigenic enteroaggregative Escherichia coli O104:H4

    Science.gov (United States)

    The Shiga toxigenic Escherichia coli O104:H4 bares the characteristics of both enterohemorrhagic (EHEC) and enteroaggregative (EAEC) E. coli. It produces plasmid encoded aggregative adherence fimbriae I (AAF/I) which mediate cell aggregation and biofilm formation in human intestine and promote Shiga...

  15. Fort Huachuca, Libby AAF, Arizona. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    Science.gov (United States)

    1982-04-22

    AAF AZ 61-70 APP STATION STATIOq NA&W YEARS UNCT PAGE 1 0900-1100 MsI" IL. S. T.) T..p.WET &ULD TEMPERATURE DEPRESION (F) TOTAL I TTL "/ 0 1-2 53 -6 I 9...PSYCHROMETRIC SUMMARY P2 AP WEATHER SERVICE/"AC 722730 FT MUACI4UCA/LhSU VLAF AZ 6-0JUL 19 PAGE 1 c90o-11jo WET WULM TUMPERATUItE DEPRESION (P) _____TOTAL...STATIO’ STATION NAME YEARS MO.T. PAGE 1 09S0-11anHO0URS L. S. T.) WET BULB TEMPERATURE DEPRESION (F) TOTAL TOTAL ( (Fl 0 1-2 3.4 5.6 It7.8 10 11112 13

  16. Inhibition of Notch signaling affects hepatic oval cell response in rat model of 2AAF-PH

    Directory of Open Access Journals (Sweden)

    Darwiche H

    2011-09-01

    Full Text Available Houda Darwiche, Seh-Hoon Oh, Nicole C Steiger-Luther, Jennifer M Williams, Dana G Pintilie, Thomas D Shupe, Bryon E PetersenDepartment of Pathology, Immunology, and Laboratory Medicine, Program in Stem Cell Biology and Regenerative Medicine, College of Medicine, University of Florida, Gainesville, FL, USABackground and aims: Activation of the oval cell compartment occurs in the liver when hepatocytes are functionally compromised and/or unable to divide. Our goal was to investigate the systemic signals responsible for determining the efficiency of oval cell-mediated liver regeneration, focusing on the Notch signaling cascade.Methods: The established oval cell induction protocol of 2-acetylaminofluorine (2-AAF implantation followed by 70% surgical resection of the liver (partial hepatectomy, PH was employed in a rat model. This oval cell induction model was further combined with injections of a γ-secretase inhibitor (GSI XX to examine the effects of Notch inhibition on oval cell-aided regeneration of the liver.Results: Notch signaling was found to be upregulated at the peak of oval cell induction during 2AAF-PH alone. Treatment with GSI XX led to interruption of the Notch signal, as shown by a decrease in expression of Hes1. While there was a robust oval cell response seen at day 11 post-PH, there was a measurable delay in differentiation when Notch was inhibited. This was confirmed morphologically as well as by immunohistochemistry for the oval cell markers, α-fetoprotein, OV-6, and CK19. The hepatocytes seen at day 22 demonstrated an enhanced hepatocellular mitoinhibition index (p21Waf1/Ki67, suggestive of dysregulated proliferation and cell cycle progression. Moreover, these hepatocytes exhibited decreased expression of hepatocyte functional markers, such as cytochrome P450 and glucose-6-phosphatase-α.Conclusion: Taken together, these results identify the Notch signaling pathway as a potent regulator of differentiation and proliferation in

  17. The fermentation stress response protein Aaf1p/Yml081Wp regulates acetate production in Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Christopher J Walkey

    Full Text Available The production of acetic acid during wine fermentation is a critical issue for wineries since the sensory quality of a wine can be affected by the amount of acetic acid it contains. We found that the C2H2-type zinc-finger transcription factor YML081Wp regulated the mRNA levels of ALD4 and ALD6, which encode a cytosolic acetaldehyde dehydrogenase (ACDH and a mitochondrial ACDH, respectively. These enzymes produce acetate from acetaldehyde as part of the pyruvate dehydrogenase bypass. This regulation was also reflected in the protein levels of Ald4p and Ald6p, as well as total ACDH activity. In the absence of ALD6, YML081W had no effect on acetic acid levels, suggesting that this transcription factor's effects are mediated primarily through this gene. lacZ reporter assays revealed that Yml081wp stimulates ALD6 transcription, in large part from a GAGGGG element 590 base pairs upstream of the translation start site. The non-annotated ORF YML081W therefore encodes a transcription factor that regulates acetate production in Saccharomyces cerevisiae. We propose AAF1 as a gene name for the YML081W ORF.

  18. Vitamin K2-enhanced liver regeneration is associated with oval cell expansion and up-regulation of matrilin-2 expression in 2-AAF/PH rat model.

    Science.gov (United States)

    Lin, M; Sun, P; Zhang, G; Xu, X; Liu, G; Miao, H; Yang, Y; Xu, H; Zhang, L; Wu, P; Li, M

    2014-03-01

    Normal liver has a great potential of regenerative capacity after partial hepatectomy. In clinic, however, most patients receiving partial hepatectomy are usually suffering from chronic liver diseases with severely damaged hepatocyte population. Under these conditions, activation of hepatic progenitor cell (oval cell in rodents) population might be considered as an alternative mean to enhance liver functional recovery. Vitamin K2 has been shown to promote liver functional recovery in patients with liver cirrhosis. In this study, we explored the possibility of vitamin K2 treatment in activating hepatic oval cell for liver regeneration with the classic 2-acetamido-fluorene/partial hepatectomy (2-AAF/PH) model in Sprague-Dawley rats. In 2-AAF/PH animals, vitamin K2 treatment induced a dose-dependent increase of liver regeneration as assessed by the weight ratio of remnant liver versus whole body and by measuring serum albumin level. In parallel, a drastic expansion of oval cell population as assessed by anti-OV6 and anti-CK19 immunostaining was noticed in the periportal zone of the remnant liver. Since matrilin-2 was linked to oval cell proliferation and liver regeneration after partial hepatectomy, we assessed its expression at both the mRNA and protein levels. The results revealed a significant increase after vitamin K2 treatment in parallel with the expansion of oval cell population. Consistently, knocking down matrilin-2 expression in vivo largely reduced vitamin K2-induced liver regeneration and oval cell proliferation in 2-AAF/PH animals. In conclusion, these data suggest that vitamin K2 treatment enhances liver regeneration after partial hepatectomy, which is associated with oval cell expansion and matrilin-2 up-regulation.

  19. Insulin-like growth factor binding protein-3 is required for the regulation of rat oval cell proliferation and differentiation in the 2AAF/PHX model

    Directory of Open Access Journals (Sweden)

    Nicole C Steiger-Luther

    2010-02-01

    RNA (siRNA treated animals determined that expression of TGFβ family members, including TGF-βRII and Smads 2–4, were significantly downregulated compared to animals at day 9 post-PHx alone or animals that received negative control siRNA. In conclusion, IGFBP-3 may function as a potent chemoattractant of oval cells during specific types of liver regeneration and may be involved in regulating oval cell proliferation and differentiation in vivo via the TGF-β pathway.Keywords: hepatic stem cells, transforming growth factor-beta, N-2-acetylaminofluorene (2AAF, partial hepatectomy (PHx

  20. 1944 AAF 661 Aerial Photo Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Aerial photographs are retrievable on a frame by frame basis. The aerial photo inventory contains imagery from various sources that are now archived at the Earth...

  1. 1943 AAF 394 Aerial Photo Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Aerial photographs are retrievable on a frame by frame basis. The aerial photo inventory contains imagery from various sources that are now archived at the Earth...

  2. 1944 AAF 649 Aerial Photo Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Aerial photographs are retrievable on a frame by frame basis. The aerial photo inventory contains imagery from various sources that are now archived at the Earth...

  3. AAFES Gas Station at Creech Air Force Base Environmental Assessment

    Science.gov (United States)

    2009-07-01

    Reservation station. Nor was there any mention of carpooling or busing to relieve the costs of the commute. Should the project be carried forward...Indian Springs, as well as the intermediate station at the Snow Mountain Piute (sic) Reservation station. Nor was there any mention of carpooling or...meet the purpose and need for this action. A sizable percentage already arrives at Creech by bus or carpool . The purpose of this action is not

  4. Limited Surface Observations Climatic Summary (LISOCS), Bo Baker AAF, Germany.

    Science.gov (United States)

    1987-11-01

    29.1 19.? 19.7 S03 CI-31 I .6 .2 I?.S 22.3 10.1 30.1 ISO 12-19 I 1. .4 iS.i ;2.C 11.2 .2 11.9 SD9 15-17 I .2 .5 13.1 21.2 IS.* .3 10.1 373 18-20 5.b...71-78,80-6 HOKIN:t C HoURSILSiI: 0600-OSCO IN ICl GE GE GE GE GE GL GE GE GE GE GE GE GE GE 6E FEET If 160 ’o 0 Go %a ISO !2 79 2c 16 12 30 a 1 9 C...60 ifge ewsaae. a. 3NTO 1OR DA6 133IS TAIN1 PBAIONAL. C. 23110111 ftCPUazm SUING 331-OM3aIMAL O.65 WILL. 361 .PLOCi Is 13333 D. 34-33 wep336P1110

  5. Environmental Assessment: Construct New AAFES Shopping Center Shaw Air Force Base, South Carolina

    Science.gov (United States)

    2008-10-01

    Wildlife expected in these open water habitats includes stocked fish such as various sunfish, bullhead catfish , largemouth bass and birds such as...arrow-head West Indian meadow-beauty Wild petunia Amphibians Northern cricket frog Reptiles Eastern coral snake Mammals Rafinesque’s big-eared

  6. Fulda AAF, Germany. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F

    Science.gov (United States)

    1972-02-08

    SPEED (FROM HOURLY OBSERVATIONS) 355 Fi,&DA GERMiN ~IY 4AF b-~-Z______ STATON STATION MAN9 ICA.$ O.N ALL EATnE., 3" ,-.5 cuss NOUAS (L s 1 SPEEDN MEAN...30,1 33,1 3346 33,3 33 O 33, 34.7 1600 0 30,4 319,2 33,3 33,3 337. 239, 11000 1 4, 2 7,2 279 213 29,9 30.1 3,4 33.1 33.4 33,3 36 3,6 ’,>_20oo2. Zola

  7. Fort Devens AAF, Massachusetts Limited Surface Observations Climatic Summary (LISOCS). Parts A-F.

    Science.gov (United States)

    1985-07-01

    NUMBER OF OBSERVATIONS - 39 0 USAETAC u 64 0-8-5 OLA PRIvo10S EIIIONS OF Y 11.5 FORM ART OSOLIET II I * SLOSAL CLINATOLo6 BRANCH USAFETAC SURFACE WINDS...51.9 51.9 OLA ... .. LG1.5AZ.._3, 1_ 5- 2. ___-52 * . 52.3 - 32.3 * 2. S2.3--52.1__ 52.3 52.352.3.-S2 .2.1-- --3.$--2.3 GE 80001 52.6 55.8 55.8 55.8...los Oita 1 9 visa 970 18 962 1 L4 0. -9 fil75 A2. 2’t 16.1 Bola 11,SaI 1-A3 $0169.:2 6 665 9. 4 36, 9.5 968 6..9.1 GE Q01 69.2 6:o.3 A6 90.2 11-6 9.6 9y

  8. The High Road to Tokyo Bay. The AAF in the Asiatic-Pacific Theater

    Science.gov (United States)

    1994-01-01

    General MacArthur would continue westward across the northern coast of New Guinea and its adjacent islands to Morotai . The two offensive arms would...In September 1944 FEAF planes supported Allied amphibi- ous forces in taking Morotai , a small, but strategically important island north of Halmahera...between New Guinea and the Philip- pines. Once Morotai became an American air base, Kenney’s aircraft were in good position to strike the East Indies

  9. Michales AAF, Dugway, Utah. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A - F

    Science.gov (United States)

    1974-03-21

    21021 24023,5 1015,1 ?o S D 9 6t" 12 50o5 94 01 l 4,4 i~ 4 ,0 I 51406,641 s2i~lU5 1 953 * _ TOTAL OBSIM 647 J2hi.k14Ji6 441 716T 6I * *61~~AQ MFAN p~ at...1019 8110 5 ti p 91 010 aus 910090171010 7,101 011017 21021 A10 23 5 1015 0 23 S D 6l25,9lr 494)0 414 4 9421 6f4;7 i2 9t6261𔄂-; TOCTAL 0851 "_t s~7jI

  10. Legislation Relating to the AAF Personnel Program, 1939 to May 1944

    Science.gov (United States)

    1944-08-01

    activo or retired lists. The letter of the Secretary of War submitting the bill called attention to the faet that if the legielation were passed, it wuIA... activo duty service, Insurame Air Corps lwrsonzel, as weOll ex imbers Of other a3ms and brinches of t’h servioe, were riven the privileoe of seeurinZ

  11. 76 FR 14421 - Section 8 Housing Assistance Payments Program-Contract Rent Annual Adjustment Factors (AAFs...

    Science.gov (United States)

    2011-03-16

    ... rent for a comparable unassisted unit of similar quality, type and age in the market area. 42 U.S.C... the unassisted market. Since HUD pays the full amount of any rent increases for assisted tenants... discount.'' Turnover is lower in assisted properties than in the unassisted market, so the effect of...

  12. Marshall AAF, Manhattan, Kansas. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    Science.gov (United States)

    1980-02-25

    1.0_ 10.7 C.3 3.6 3.7 .0 .0 .0 .0 .0 .0 .0 3.6 Q.2 26.4 5.7, 2.7 3.0 .8 .0 .0 .0 .0 . 3.4 1.5 17.1 ,.0 21.7 17.5 TRAC [ . 0 . ,. .0 .0 .1 3.. It., 1.’ 7.2...11 13. 8. - / *,_ i_ I , ! ZII -i __ __ __ __ ___ __ __ __ __ __ . . 12 0 ’, E I 3 F A 6 7 1 I 0 3 1 I -:-. c . B P t e 1 • - t -- _ _ _ _ I , vtt

  13. Separate and Unequal: Race Relations in the AAF During World War 2

    Science.gov (United States)

    2000-01-01

    martialed the colonel. The Air Surgeon asked a consultant, Dr. Lawrence A. Kubie , who had been conducting a psychiatric study of black pilots at Selfridge...and Oscoda, to examine the colonel. Dr. Kubie went beyond his charter and made some interesting observations about racial conditions at the two...grew deeper and more charged as the war progressed. It followed that minor incidents appeared to provoke major racial disturbances. Kubie found rap

  14. Gray AAF, Fort Lewis, Lacey, Washington. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F

    Science.gov (United States)

    1979-02-23

    o. __,<_ WNW 4UBRO 4.5B . 9VAIOSI),, ___5_ 38__6 8.7 .4__ 110m_ _ ________ __ _____TOTA NUBE OF OBSERVAT---IOS 1 SWFEA JU 4 0 O PI..’ r ju...i _ _ _, _ _ SEW . .. .. _ _ I __ ___ _ _ ___ _ VESL I* .11 I ’ : __ __ __ 4 ) 1..d __ _ ! _____ ___,_ ______... Rl.O ,,3 TOTA- -izI NUBE OF...squares (ZX2), suns of values (ZX), meens (X), and standard deviations (ax). The number of obser- vations used in the computaCions for each elc.,er

  15. Fritzsche AAF, Fort Ord, Salinas, California. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    Science.gov (United States)

    1972-02-17

    0’- ,) 7,5 9b , 41 9- ’- , =’ "’"~~~~~ 993 99,3 99,8 9,I, 0~ . hS100 ’,It ’-s -7) 7771, *- ; 77 ’ .’ - 7 99-- 99 .~ dc~ g If2: 0 k 4.94, 1 9 , 7. C

  16. Final Environmental Assessment: Addressing An Army and Air Force Exchange Service (AAFES) Lifestyle Center at Eglin Air Force Base, Florida

    Science.gov (United States)

    2008-11-01

    Federal agencies that promotes environmental practices, including acquisition of biobased , environmentally preferable, energy-efficient, water...of the area; these items include beer cans, bottles, plastic , etc. Report Organization Following the introduction is a brief overview of the

  17. Fort Knox/Godman AAF, Kentucky. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    Science.gov (United States)

    1982-07-13

    88.6 88.6 88.6 83.6 88.6 88.6 ea .6 52.9 53. 58. 56. 58.2 60.2 64.1 65 67.2 69.C 69. 9 71 73.1 78.6 81.9 84.3 86. 88.7 89.6...ISSHT |T.«|I«ID«| 885 11. 1 77.6 i i • / < 0 i ’is j I ( J A L C • tlTAC A . EA 7 r2 2*c STATION...59.3 63.8 62.1 56.5 «2.9 33.9 26.7 «3.3 so 1«.<» 9313 . 1<»812. Pill. 377 9.«68 6.991 5.917 6.6«« 8 . 5051 Z. 83812

  18. Gray AAF/Fort Lewis, Washington. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    Science.gov (United States)

    1982-12-14

    with dust ad/or sand is included in this summary only when visibility is reduced to A- 3 / TLAL CLT vTCLC3Y DPOH " , AT-r, SEQvI’T/ AO STATJCN NAME , EA ;s...93.5 94 .. .. 95.I 95.9 96.5 97. .97.7 97.8 98 .1 98.2 , 99.,.,. TOTAk NU BER OF OSFVATION, - -- -- - - -- LCEILING VERSUS VISIBILITY T - S Rv. F EA ...88.6 88.6 868.6 88.6 88.6 88.6 88.6 88.6 R8.6 5 . _,𔃿, , 93.1 9 3. 1 .. 93o41. 3 9313 93.3 93.3 1 9𔃻.13 91.3 93.3 9.3-1A-2.lT -5.3 ;3.9 95.5 95.7 9S7

  19. Fort Campbell, Campbell AAF, Kentucky. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    Science.gov (United States)

    1982-12-01

    R SERVICE/MAC PERCENTAGE FREQUENCY OF WIND DIRECTION AND SPEED (FROM HOURLY OBSERVATIONS) ,4671 FT CAMPRELL KY 73-81 "A-’ ALL WEATHtR. SPEED MAN...34., AFETAC SURFACE WINDS A2 EATHR SEV1CL/MAC PERCENTAGE FREQUENCY OF WIND DIRECTION AND SPEED (FROM HOURLY OBSERVATIONS) 74f-71 FT CAMPRELL KY 73-81...SERVI11/mAC ’ 467 . FT CAMPRELL KY 73-31 Ju’. e PERCENTAGE FREQUENCY OF OCCURRENCE (FROM HOURLY OBSERVATIONS) VfSf bitT STAT JTE -ILES !Ci Nu, i I -o i > I

  20. Army Air Force Exchange Service Service (AAFES) Station Tyndall Air Force Base, Florida Final Tiered Environmental Assessment

    Science.gov (United States)

    2010-04-20

    Shoppette/ Gas Station at Tyndal l AFB. Florida. Th is project is located just inside the Main Gate on Ill inois Avenue (Figure 2). The Shoppette will...include a retai l store. class six, and a Blimpics. The proposed prc~jec t would consist of one bui lding. a canopied gas di spensing island and paved...ze two underground storag~: tanks f()r storage of tv.:o grades of unleaded fue l. Tank sizes ’"’·ould he 20.000 Regular Unleaded and 15.000 Premium

  1. Biggs AAF, El Paso, Texas. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F

    Science.gov (United States)

    1981-01-14

    BGSAF E AO 38 U -OOTN FRO HORL OLOBLECIMAOLOYIONS Hoti !S WEATHER CONDITLOONS MONTH CRMS I AD/OR jRAIN &,cRj ANDOR MAIL -B A’ D/OR WITH 015? NO. OF (L5 W...0_____ Fs 32_FJa067 F 73F 0 F -93 F Total . -,-.. ;. .." "I ~ q " -," " * ’f 41 v>"K~ ’ ’, ,Jim j - - ’ ArTA C PSYCHROMETRIC SUMMARY L. < el’ AT.Ft? SUIV

  2. Metabolism of 2-acetylaminofluorene by Shasta rainbow trout

    Energy Technology Data Exchange (ETDEWEB)

    Sikka, H.C.; Elmarakaby, S.A.; Steward, R.; Maslanka, R.; Shappell, N.; Kumar, S. [State University of New York, Buffalo, NY (United States); Devanaboyina, U.; Gupta, R.C. [Univ. of Kentucky, Lexington, KY (United States)

    1994-12-31

    In contrast to several mammalian species, Shasta strain rainbow trout (Oncorhynchus mykiss), is resistant to the hepatocarcinogenic effects of 2-acetylaminofluorene (AAF). In order to understand the mechanism underlying this resistance, the authors have investigated the in vitro metabolism of AAF by trout liver and examined the formation of AAF-DNA adducts in the liver of Shasta trout treated with AAF. The major AAF metabolites produced by trout liver microsomes were 7-hydroxy-AAF and 5-hydroxy-AAF which accounted for more than 95% of the total AAF metabolites. N-hydroxy-AAF was a minor metabolite representing about 1% of total AAF metabolites. The levels of N-hydroxy-AAF sulfotransferase and N-hydroxy-AAF acyltransferase, the cytosolic enzymes implicated in the metabolic activation of N-hydroxy-AAF to reactive intermediates capable of binding to cellular macromolecules were extremely low in trout liver. AAF exhibited a low degree of binding to the liver DNA of trout treated with 15 mg AAF/kg body wt. The total AAF-DNA adduct level reached a maximum 24 hours after treatment, persisted until 11 days and declined to nearly 20% of the maximum level after 18 days. N-deoxyguanosin-8-yl-2-aminofluorene was the major AAF-DNA adduct in the trout liver. The ability of trout liver to form relatively large amounts of detoxification products of AAF with little formation of activation products may partly explain the resistance of Shasta trout to AAF-induced hepatocarcinogenesis.

  3. The AAF in Northwest Africa. An Account of the Twelfth Air Force in the Northwest African Landing and the Battle for Tunisia

    Science.gov (United States)

    1992-01-01

    ones hastily laid out in wheat fields and sheep pastures. Supplies at first were a problem, but air service, quartermaster, and ordnance organizations...equipped men. Not only did the enemy have the advantage of being within ioo miles of his Sicilian bases, across a strip of water which he rigidly...bombers were strewing havoc among supply ships out of Naples. The enemy then took to sending ships out of Palermo and other Sicilian ports to make the run

  4. NCBI nr-aa BLAST: CBRC-OLAT-26-0097 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-26-0097 gb|AAF05731.1| putative ultraviolet sensitive opsin [Dimidiochrom...is compressiceps] gb|AAF05733.1| putative ultraviolet sensitive opsin [Metriaclima zebra] gb|AAF05734.1| putative ultra...violet sensitive opsin [Labeotropheus fuelleborni] gb|AAZ53204.1| putative ultraviolet sensitive visual pigment [Tramitichromis intermedius] AAF05731.1 1e-171 85% ...

  5. NCBI nr-aa BLAST: CBRC-OGAR-01-0515 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OGAR-01-0515 gb|AAF89359.1|AF172239_1 chemokine receptor CXCR4 [Nycticebus cou...cang] gb|AAF89360.1|AF172240_1 chemokine receptor CXCR4 [Nycticebus intermedius] gb|AAF89361.1|AF172241_1 chemokine receptor CXCR4 [Nycticebus pygmaeus] AAF89359.1 0.0 96% ...

  6. The Joint Staff/J-5 and A-AF CLIC (Center for Low Intensity Conflict) Planning and Policy in Low Intensity Conflict Conference held in Hampton, Virginia on 13-15 December 1988

    Science.gov (United States)

    1988-12-15

    that "LIC" is a strategy of conflict, where dilatory tactics are employed with increasing violence to wear down the opponent. As Jean - Paul Sartre put...Defense for Drug Policy and Enforcement; and Mr. Paul Mahlstedt, Aide to General Paul F. Gorman, USA (Retired) (Ballroom) 0830-1230 Reserve Affairs...General Paul F. Gorman, former Commander-in-Chief of U.S. Southern Command, and the principal architect of two of the three reports I’ve just cited

  7. Structural insight into host recognition by aggregative adherence fimbriae of enteroaggregative Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Andrea A Berry

    2014-09-01

    Full Text Available Enteroaggregative Escherichia coli (EAEC is a leading cause of acute and persistent diarrhea worldwide. A recently emerged Shiga-toxin-producing strain of EAEC resulted in significant mortality and morbidity due to progressive development of hemolytic-uremic syndrome. The attachment of EAEC to the human intestinal mucosa is mediated by aggregative adherence fimbria (AAF. Using X-ray crystallography and NMR structures, we present new atomic resolution insight into the structure of AAF variant I from the strain that caused the deadly outbreak in Germany in 2011, and AAF variant II from archetype strain 042, and propose a mechanism for AAF-mediated adhesion and biofilm formation. Our work shows that major subunits of AAF assemble into linear polymers by donor strand complementation where a single minor subunit is inserted at the tip of the polymer by accepting the donor strand from the terminal major subunit. Whereas the minor subunits of AAF have a distinct conserved structure, AAF major subunits display large structural differences, affecting the overall pilus architecture. These structures suggest a mechanism for AAF-mediated adhesion and biofilm formation. Binding experiments using wild type and mutant subunits (NMR and SPR and bacteria (ELISA revealed that despite the structural differences AAF recognize a common receptor, fibronectin, by employing clusters of basic residues at the junction between subunits in the pilus. We show that AAF-fibronectin attachment is based primarily on electrostatic interactions, a mechanism not reported previously for bacterial adhesion to biotic surfaces.

  8. ACCELERATION AMBIGUITY FUNCTION OF RADAR SIGNALS AND ITS APPLICATION

    Institute of Scientific and Technical Information of China (English)

    Zhao Hongzhong; Zhou Jianxiong; Li Weimin; Fu Qiang

    2003-01-01

    High acceleration of radar targets is analyzed using Acceleration Ambiguity Function (AAF). The acceleration resolution based on AAF is defined. The AAF and acceleration resolution of rectangle pulse signal are derivated and the conclusion that its acceleration resolution is in inverse proportion with the square of its duration is drawn. In the end, these conclusions are applied to the parameter designing and performance evaluation for a certain type of pulse Doppler radar.

  9. Identification of cell surface-exposed proteins involved in the fimbria-mediated adherence of enteroaggregative Escherichia coli to intestinal cells.

    Science.gov (United States)

    Izquierdo, Mariana; Navarro-Garcia, Fernando; Nava-Acosta, Raul; Nataro, James P; Ruiz-Perez, Fernando; Farfan, Mauricio J

    2014-04-01

    Fimbria-mediated adherence to the intestinal epithelia is a key step in enteroaggregative Escherichia coli (EAEC) pathogenesis. To date, four fimbriae have been described for EAEC; aggregative adherence fimbria II (AAF/II) is the most important adherence factor for EAEC prototype strain 042. Previously, we described results showing that extracellular matrix (ECM) components might be involved in the recognition of AAF/II fimbriae by intestinal cells. In this study, we sought to identify novel potential receptors on intestinal epithelial cells recognized by the AAF/II fimbriae. Purified AafA-dsc protein, the major subunit of AAF/II fimbriae, was incubated with a monolayer of T84 cells, cross-linked to the surface-exposed T84 cell proteins, and immunoprecipitated by using anti-AafA antibodies. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of cellular proteins bound to AafA-dsc protein identified laminin (previously recognized as a potential receptor for AAF/II) and cytokeratin 8 (CK8). Involvement of the major subunit of AAF/II fimbriae (AafA protein) in the binding to recombinant CK8 was confirmed by adherence assays with purified AAF/II fimbriae, AafA-dsc protein, and strain 042. Moreover, HEp-2 cells transfected with CK8 small interfering RNA (siRNA) showed reduced 042 adherence compared with cells transfected with scrambled siRNA as a control. Adherence of 042 to HEp-2 cells preincubated with antibodies against ECM proteins or CK8 was substantially reduced. Altogether, our results supported the idea of a role of CK8 as a potential receptor for EAEC.

  10. Comparisons of Stuttering Frequency during and after Speech Initiation in Unaltered Feedback, Altered Auditory Feedback and Choral Speech Conditions

    Science.gov (United States)

    Saltuklaroglu, Tim; Kalinowski, Joseph; Robbins, Mary; Crawcour, Stephen; Bowers, Andrew

    2009-01-01

    Background: Stuttering is prone to strike during speech initiation more so than at any other point in an utterance. The use of auditory feedback (AAF) has been found to produce robust decreases in the stuttering frequency by creating an electronic rendition of choral speech (i.e., speaking in unison). However, AAF requires users to self-initiate…

  11. Scientist and Engineer Career Patterns for Air Force Civilians and Officers

    Science.gov (United States)

    1999-04-01

    Wendy Campbell, Colonel T. Jan Cerveny, Mr. John Miner, and Mr. Scott Petroski . vii AU/ACSC/029/1999-04 Abstract Technology will play a critical role in...Commanding General of the Army Air Forces (AAF), General of the Army Henry Harley “Hap” Arnold, was integral in his decision to activate the AAF Scientific

  12. NCBI nr-aa BLAST: CBRC-OGAR-01-0612 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-152 88% ... ...CBRC-OGAR-01-0612 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  13. NCBI nr-aa BLAST: CBRC-CPOR-01-0920 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-139 79% ... ...CBRC-CPOR-01-0920 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  14. NCBI nr-aa BLAST: CBRC-OGAR-01-0618 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 3e-77 64% ... ...CBRC-OGAR-01-0618 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  15. NCBI nr-aa BLAST: CBRC-DNOV-01-2223 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-138 79% ... ...CBRC-DNOV-01-2223 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  16. NCBI nr-aa BLAST: CBRC-HSAP-17-0013 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-170 95% ... ...CBRC-HSAP-17-0013 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  17. NCBI nr-aa BLAST: CBRC-BTAU-01-1776 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-147 82% ... ...CBRC-BTAU-01-1776 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  18. NCBI nr-aa BLAST: CBRC-CPOR-01-0063 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-69 57% ... ...CBRC-CPOR-01-0063 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  19. NCBI nr-aa BLAST: CBRC-EEUR-01-0490 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-147 83% ... ...CBRC-EEUR-01-0490 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  20. NCBI nr-aa BLAST: CBRC-CJAC-01-0087 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 8e-91 64% ... ...CBRC-CJAC-01-0087 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  1. NCBI nr-aa BLAST: CBRC-CJAC-01-1044 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-122 80% ... ...CBRC-CJAC-01-1044 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  2. NCBI nr-aa BLAST: CBRC-PABE-18-0002 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-137 78% ... ...CBRC-PABE-18-0002 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  3. NCBI nr-aa BLAST: CBRC-FCAT-01-0270 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-139 78% ... ...CBRC-FCAT-01-0270 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  4. NCBI nr-aa BLAST: CBRC-BTAU-01-1761 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-149 84% ... ...CBRC-BTAU-01-1761 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  5. NCBI nr-aa BLAST: CBRC-BTAU-01-1764 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-149 83% ... ...CBRC-BTAU-01-1764 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  6. NCBI nr-aa BLAST: CBRC-BTAU-01-1765 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-150 84% ... ...CBRC-BTAU-01-1765 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  7. NCBI nr-aa BLAST: CBRC-PABE-18-0015 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-126 95% ... ...CBRC-PABE-18-0015 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  8. NCBI nr-aa BLAST: CBRC-HSAP-17-0002 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-126 76% ... ...CBRC-HSAP-17-0002 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  9. NCBI nr-aa BLAST: CBRC-CFAM-41-0069 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-150 85% ... ...CBRC-CFAM-41-0069 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  10. NCBI nr-aa BLAST: CBRC-CJAC-01-1241 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-106 68% ... ...CBRC-CJAC-01-1241 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  11. NCBI nr-aa BLAST: CBRC-ETEL-01-0768 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available tory receptor [Gorilla gorilla] gb|AAF03337.1| olfactory receptor [Gorilla gorilla] Q9TQX4 1e-156 88% ... ...CBRC-ETEL-01-0768 sp|Q9TQX4|OR1E2_GORGO Olfactory receptor 1E2 gb|AAF03336.1| olfac

  12. Gunshot-Induced Aorto-Left Atrial Fistula Diagnosed by Intraoperative Transesophageal Echocardiography.

    Science.gov (United States)

    Nandate, Koichiro; Krishnamoorthy, Vijay; McIntyre, Lisa K; Verrier, Edward D; Mackensen, G Burkhard

    2016-02-01

    Aorto-left atrial fistula (AAF) is rarely encountered in clinical practice, and the early diagnosis can be very challenging. This report describes a unique case of AAF caused by a gunshot injury and the pivotal role of transesophageal echocardiography for diagnosis and assessment.

  13. Suppressive effect of herbal compound 861 on chemical hepatocarcinogenesis in rats

    Institute of Scientific and Technical Information of China (English)

    崔儒涛; 王宝恩; 张福奎; 陈光勇; 尹珊珊

    2003-01-01

    Objective To investigate the effects of herbal compound 861 (Cpd861) on hepatocarcinogenesis induced by diethylntrosamine and 2-acetylaminofluorene (DEN-AAF) in female Sprague Dawley rats.Methods Liver preneoplastic foci were induced using the DEN-AAF method in female Sprague Dawley rats, which were then treated with Cpd861. For quantitative assessment of liver preneoplastic foci, the placental form of glutathione-S-transferase (GST-P) positive foci were measured using immunohistochemical staining and image analysis. GST-P protein expression was measured by Western blotting, mRNA expression was assessed by Northern blotting.Results Treatment using DEN-AAF caused a significant decrease in body weight and increase in liver weight compared to the control group. Oral Cpd861 administration essentially prevented DEN-AAF-induced body weight loss and liver weight increase. When 2-AAF was followed by treatment with Cpd861, there was a decrease in the number of large foci as compared to 2-AAF alone. However, there were still considerable numbers of small mixed clear/vacuolated cell foci, some of which were positive for GST-P. Significant increase in GST-P protein and mRNA expression were observed in the DEN-AAF group, while treatment with Cpd861 inhibited the increase. The effect of Cpd861 on hepatocarcinogenesis occurred in a concentration-dependent manner. Conclusion Chinese herbal compound Cpd861 prevents hepatocarcinogenesis in DEN-AAF-induced liver preneoplastic lesions in rats.

  14. 77 FR 22340 - Section 8 Housing Assistance Payments Program-Annual Adjustment Factors, Fiscal Year 2012

    Science.gov (United States)

    2012-04-13

    ... Statistics Consumer Price Index (CPI) survey. These factors are applied at Housing Assistance Payment (HAP... no need to differentiate the AAF by use. A separate Federal Register Notice will be published that.... There are three categories of Section 8 programs that use the AAFs: Category 1--The Section 8...

  15. 78 FR 30322 - Section 8 Housing Assistance Payments Program-Annual Adjustment Factors, Fiscal Year 2013

    Science.gov (United States)

    2013-05-22

    ... Statistics Consumer Price Index (CPI) survey. These factors are applied at Housing Assistance Payment (HAP... there is no need to differentiate the AAF by use. A separate Federal Register Notice will be published... program. There are three categories of Section 8 programs that use the AAFs: Category 1: The Section 8...

  16. 维生素K2促进大鼠肝再生模型肝切除术后肝功能恢复的研究%Positive effect of vitamin K2 on recovery of liver function in liver regeneration rats model after hepatectomy

    Institute of Scientific and Technical Information of China (English)

    张谷裕; 刘国华; 林满洲; 李明意; 杨永光; 许浩

    2013-01-01

    Objective To investigate the positive effect of the vitamin K2 on recovery of liver function in the rat liver regeneration process. Methods 180 SD rats were randomly divided into six groups including five experimental groups: (1) 2AAF+VLVK2/PH, (2) 2AAF+LVK/PH, (3) 2AAF+MVK/PH, (4) 2AAF+HVK/ PH, (5) 2AAF+VHVK/PH, and control group: 2AAF/PH. The 70% partial hepatectomy (PH) were performed on the day 4, 8, 12, 16 and 20. The levels of ALT, AST, ALB in blood serum were examined. Results Rats in 2AAF/PH group had significantly higher ALT, AST and a markedly lower level of ALB than other experimental groups on the day 4, 8(P 0.05 ). Conclusion Vitamin K2 can promote the recovery of rats liver function, and when dose were lower than 20 mg/ kg, the effect was dose dependent.%目的:通过建立大鼠肝卵圆细胞增殖肝再生模型.探讨维生素K2对大鼠肝再生模型部分肝切除术后肝再生过程中肝功能恢复的影响.方法:180只SD雄性大鼠随机分为6组,实验组5组:(1)2AAF+VLVK2/PH,(2)2AAF+LVK2/PH,(3)2AAF+MVK2/PH,(4)2AAF+HVK2/PH,(5)2AAF+VHVK2/PH;对照组:2AAF/PH.分别于术后第4、8、12、16、20天采血检测血清天门冬氨酸氨基转移酶(AST)、血清丙氨酸氨基转移酶(ALT)、白蛋白(ALB)的动态变化情况.结果:2AAF/PH组第4、8天ALT、AST与5组维生素K2不同剂量实验组相比较明显升高(P0.05).结论:维生素K2对大鼠肝再生模型术后肝功能恢复有明显的改善作用;当剂量低于20 mg/kg时,肝功能恢复呈剂量依赖性.

  17. Analysis of heat pumps installed in family housing at Hunter Army Air Field

    Energy Technology Data Exchange (ETDEWEB)

    Parker, S.A.

    1994-08-01

    The US Army Forces Command (FORSCOM) tasked Pacific Northwest Laboratory (PNL) in support of the US Department of Energy (DOE) Federal Energy Management Program (FEMP) to conduct a postconstruction evaluation of the air-source heat pumps installed in family housing at Hunter Army Air Field (AAF). The objective of this project was to investigate and resolve concerns about an increase in energy costs at Hunter AAF following the installation of heat pumps in November 1992. After completing several analyses and a field inspection of the heat pumps in family housing at Hunter AAF, the following conclusions were made: the installation of air-source heat pumps reduced the annual energy cost in family housing by $46,672 in 1993; the heat pump thermostat controls in Hunter AAF family housing appear to be incorrectly installed; and the Hunter AAF electric utility bill increased 10% during the first 6 months of 1993 compared to the first 6 months of 1992.

  18. Bacterial dynamics and metabolite changes in solid-state acetic acid fermentation of Shanxi aged vinegar.

    Science.gov (United States)

    Li, Sha; Li, Pan; Liu, Xiong; Luo, Lixin; Lin, Weifeng

    2016-05-01

    Solid-state acetic acid fermentation (AAF), a natural or semi-controlled fermentation process driven by reproducible microbial communities, is an important technique to produce traditional Chinese cereal vinegars. Highly complex microbial communities and metabolites are involved in traditional Chinese solid-state AAF, but the association between microbiota and metabolites during this process are still poorly understood. In this study, we performed amplicon 16S rRNA gene sequencing on the Illumina MiSeq platform, PCR-denaturing gradient gel electrophoresis, and metabolite analysis to trace the bacterial dynamics and metabolite changes under AAF process. A succession of bacterial assemblages was observed during the AAF process. Lactobacillales dominated all the stages. However, Acetobacter species in Rhodospirillales were considerably accelerated during AAF until the end of fermentation. Quantitative PCR results indicated that the biomass of total bacteria showed a "system microbe self-domestication" process in the first 3 days, and then peaked at the seventh day before gradually decreasing until the end of AAF. Moreover, a total of 88 metabolites, including 8 organic acids, 16 free amino acids, and 66 aroma compounds were detected during AAF. Principal component analysis and cluster analyses revealed the high correlation between the dynamics of bacterial community and metabolites.

  19. The DOE ARM Aerial Facility

    Energy Technology Data Exchange (ETDEWEB)

    Schmid, Beat; Tomlinson, Jason M.; Hubbe, John M.; Comstock, Jennifer M.; Mei, Fan; Chand, Duli; Pekour, Mikhail S.; Kluzek, Celine D.; Andrews, Elisabeth; Biraud, S.; McFarquhar, Greg

    2014-05-01

    The Department of Energy Atmospheric Radiation Measurement (ARM) Program is a climate research user facility operating stationary ground sites that provide long-term measurements of climate relevant properties, mobile ground- and ship-based facilities to conduct shorter field campaigns (6-12 months), and the ARM Aerial Facility (AAF). The airborne observations acquired by the AAF enhance the surface-based ARM measurements by providing high-resolution in-situ measurements for process understanding, retrieval-algorithm development, and model evaluation that are not possible using ground- or satellite-based techniques. Several ARM aerial efforts were consolidated into the AAF in 2006. With the exception of a small aircraft used for routine measurements of aerosols and carbon cycle gases, AAF at the time had no dedicated aircraft and only a small number of instruments at its disposal. In this "virtual hangar" mode, AAF successfully carried out several missions contracting with organizations and investigators who provided their research aircraft and instrumentation. In 2009, AAF started managing operations of the Battelle-owned Gulfstream I (G-1) large twin-turboprop research aircraft. Furthermore, the American Recovery and Reinvestment Act of 2009 provided funding for the procurement of over twenty new instruments to be used aboard the G-1 and other AAF virtual-hangar aircraft. AAF now executes missions in the virtual- and real-hangar mode producing freely available datasets for studying aerosol, cloud, and radiative processes in the atmosphere. AAF is also engaged in the maturation and testing of newly developed airborne sensors to help foster the next generation of airborne instruments.

  20. Exploring microbial succession and diversity during solid-state fermentation of Tianjin duliu mature vinegar.

    Science.gov (United States)

    Nie, Zhiqiang; Zheng, Yu; Wang, Min; Han, Yue; Wang, Yuenan; Luo, Jianmei; Niu, Dandan

    2013-11-01

    Tianjin duliu mature vinegar was one of famous Chinese traditional vinegars. The unique flavor and taste of vinegar are mainly generated by the multitudinous microorganisms during fermentation. In this research, the composition and succession of microbial communities in the entire solid-state fermentation were investigated, including starter daqu and acetic acid fermentation (AAF). Molds and yeasts in daqu, including Aspergillus, Saccharomycopsis and Pichia, decreased in AAF. The bacterial compositions increased from four genera in daqu to more than 13 genera in AAF. Principal component analysis showed that Acetobacter, Gluconacetobacter, Lactobacillus and Nostoc were dominant bacteria that were correlated well with AAF process. In the early fermentation period, lactic acid bacteria (LAB) decreased while acetic acid bacteria and Nostoc increased rapidly with the accumulation of total acids. Then, the abundance and diversity of LAB increased (more than 80%), indicating that LAB had important influences on the flavor and taste of vinegar.

  1. Annual Adjustment Factors

    Data.gov (United States)

    Department of Housing and Urban Development — The Department of Housing and Urban Development establishes the rent adjustment factors - called Annual Adjustment Factors (AAFs) - on the basis of Consumer Price...

  2. Which type of forest management provides most ecosystem services?

    OpenAIRE

    Timo Pukkala

    2016-01-01

    Background: Forest ecosystems are increasingly seen as multi-functional production systems, which should provide, besides timber and economic benefits, also other ecosystem services related to biological diversity, recreational uses and environmental functions of forests. This study analyzed the performance of even-aged rotation forest management (RFM), continuous cover forestry (CCF) and any-aged forestry (AAF) in the production of ecosystem services. AAF allows both even-aged an...

  3. NCBI nr-aa BLAST: CBRC-OANA-01-2156 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-2156 gb|AAF87984.1|AF252554_1 CC chemokine receptor 5 [Callithrix jacc...hus] gb|AAK43361.1|AF177878_1 C-C chemokine receptor 5 [Callithrix jacchus] gb|AAN14530.1|AF452614_1 chemokine receptor CCR5 [Call...ithrix jacchus] gb|AAQ20011.1| CC chemokine receptor 5 [Callithrix jacchus] AAF87984.1 1e-145 69% ...

  4. NCBI nr-aa BLAST: CBRC-OANA-01-2157 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-2157 gb|AAF87984.1|AF252554_1 CC chemokine receptor 5 [Callithrix jacc...hus] gb|AAK43361.1|AF177878_1 C-C chemokine receptor 5 [Callithrix jacchus] gb|AAN14530.1|AF452614_1 chemokine receptor CCR5 [Call...ithrix jacchus] gb|AAQ20011.1| CC chemokine receptor 5 [Callithrix jacchus] AAF87984.1 1e-141 70% ...

  5. NCBI nr-aa BLAST: CBRC-CJAC-01-0968 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0968 gb|AAF87984.1|AF252554_1 CC chemokine receptor 5 [Callithrix jacc...hus] gb|AAK43361.1|AF177878_1 C-C chemokine receptor 5 [Callithrix jacchus] gb|AAN14530.1|AF452614_1 chemokine receptor CCR5 [Call...ithrix jacchus] gb|AAQ20011.1| CC chemokine receptor 5 [Callithrix jacchus] AAF87984.1 0.0 100% ...

  6. NCBI nr-aa BLAST: CBRC-TBEL-01-2360 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TBEL-01-2360 gb|AAF87984.1|AF252554_1 CC chemokine receptor 5 [Callithrix jacc...hus] gb|AAK43361.1|AF177878_1 C-C chemokine receptor 5 [Callithrix jacchus] gb|AAN14530.1|AF452614_1 chemokine receptor CCR5 [Call...ithrix jacchus] gb|AAQ20011.1| CC chemokine receptor 5 [Callithrix jacchus] AAF87984.1 1e-161 77% ...

  7. Tolerance to a new free amino acid-based formula in children with IgE or non-IgE-mediated cow’s milk allergy: a randomized controlled clinical trial

    Directory of Open Access Journals (Sweden)

    Berni Canani Roberto

    2013-02-01

    Full Text Available Abstract Background Amino acid-based formulas (Aaf are increasingly used in children with cow’s milk allergy (CMA. To be labeled hypoallergenic these formulas must demonstrate in clinical studies that they don’t provoke reactions in 90% of subjects with confirmed CMA with 95% confidence when given in prospective randomized, double-blind, placebo-controlled challenge (DBPCFC trials. The majority of available safety data on Aaf derived from patients with IgE-mediated CMA. Considering substantial differences in the immunologic mechanism and clinical presentation of non-IgE-mediated CMA it’s important to investigate the hypoallergenicity of these formulas also in these patients. We prospectively assessed the tolerance to a new commercially available Aaf in children affected by IgE- or non-IgE-mediated CMA. Methods Consecutive patients affected by IgE- or non-IgE-mediated CMA, aged ≤ 4 years, were enrolled. DBPCFC was carried out with increasing doses of the new Aaf (Sineall, Humana, Milan, Italy, using validated Aaf as placebo. Faecal concentrations of calprotectin (FC and eosinophilic cationic protein (ECP were monitored. Results Sixty patients (44 male, 73.3%, median age 37, 95%CI 34.5–39.6 months, IgE-mediated CMA 29, 48.3% were enrolled. At the diagnosis clinical symptoms were gastrointestinal (46.6%, cutaneous (36.6%, respiratory (23.3%, and systemic (10.0%. After DBPCFC with the new Aaf, no patient presented early or delayed clinical reactions. Faecal concentration of calprotectin and of ECP remained stable after the exposure to the new Aaf. Conclusions The new Aaf is well tolerated in children with IgE- or non-IgE-mediated CMA, and it could be used as a safe dietotherapy regimen for children with this condition. Trial registration The trial was registered in the ClinicalTrials.gov Protocol Registration System (ID number: NCT01622426.

  8. An experimental method for rapid growth of liver in spleen. The survival and proliferation of chemically induced preneoplastic hepatocytes in spleen.

    OpenAIRE

    Finkelstein, S. D.; Lee, G.; Medline, A.; Tatematsu, M; Makowka, L.; Farber, E.

    1983-01-01

    Cellular suspensions (2 x 10(6) cells) of isolated preneoplastic liver cells, obtained from carcinogen-treated rats, were injected in the spleens of syngeneic rats divided into groups on the basis of no treatment, partial hepatectomy (PH), and/or feeding regimens including 2-acetylaminofluorene (AAF). Recipient rats undergoing both PH and AAF showed significantly more rapid proliferation of the preneoplastic liver cell implant, compared with other treatment groups and control. The theoretic b...

  9. Amplified somatosensory and visual cortical projections to a core auditory area, the anterior auditory field, following early- and late-onset deafness.

    Science.gov (United States)

    Wong, Carmen; Chabot, Nicole; Kok, Melanie A; Lomber, Stephen G

    2015-09-01

    Cross-modal reorganization following the loss of input from a sensory modality can recruit sensory-deprived cortical areas to process information from the remaining senses. Specifically, in early-deaf cats, the anterior auditory field (AAF) is unresponsive to auditory stimuli but can be activated by somatosensory and visual stimuli. Similarly, AAF neurons respond to tactile input in adult-deafened animals. To examine anatomical changes that may underlie this functional adaptation following early or late deafness, afferent projections to AAF were examined in hearing cats, and cats with early- or adult-onset deafness. Unilateral deposits of biotinylated dextran amine were made in AAF to retrogradely label cortical and thalamic afferents to AAF. In early-deaf cats, ipsilateral neuronal labeling in visual and somatosensory cortices increased by 329% and 101%, respectively. The largest increases arose from the anterior ectosylvian visual area and the anterolateral lateral suprasylvian visual area, as well as somatosensory areas S2 and S4. Consequently, labeling in auditory areas was reduced by 36%. The age of deafness onset appeared to influence afferent connectivity, with less marked differences observed in late-deaf cats. Profound changes to visual and somatosensory afferent connectivity following deafness may reflect corticocortical rewiring affording acoustically deprived AAF with cross-modal functionality.

  10. Chemoprotective effect of Vernonia amygdalina Del. (Astereacea) against 2-acetylaminofluorene-induced hepatotoxicity in rats.

    Science.gov (United States)

    Adesanoye, Omolola A; Adekunle, Abiodun E; Adewale, Olusola B; Mbagwu, Abraham E; Delima, Adetutu A; Adefegha, Stephen A; Molehin, Olorunfemi R; Farombi, Ebenezer O

    2016-01-01

    Natural products possessing antioxidant properties play a very crucial role in ameliorating deleterious effects of reactive oxygen species. This study investigated the chemoprotective properties of methanolic extract of Vernonia amygdalina (MEVA) in an experimental model of hepatic oxidative damage induced by 2-acetylaminofluorene (2-AAF). Rats were divided into six groups. Groups 1 and 2 received saline and dimethyl sulfoxide, respectively, and served as controls. Group 3 received MEVA at a dose of 250 mg/kg, while groups 5 and 6 were pretreated for 14 days with MEVA at 250 mg/kg and 500 mg/kg doses before coadministration with 2-AAF at 100 mg/kg for another 7 days. 2-AAF was administered to group 4 for the last 7 days. Animals were killed 24 h after the last administration of 2-AAF. 2-AAF significantly (p induced marked hepatic damage as revealed by increased activities of serum enzymes such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and γ-glutamyl transferase and bilirubin concentration. 2-AAF also elicited decrease in the activities of antioxidant enzymes such as superoxide dismutase, catalase, glutathione-S-transferase, and glutathione peroxidase, depletion of reduced glutathione, and increase in malondialdehyde levels. The activities of glucose-6-phosphatase and 5'-nucleotidase were also depleted. MEVA at 250 mg/kg and 500 mg/kg significantly (p induced toxicity may be due to its antioxidant and free-radical scavenging activities, thus suggesting its usefulness as a possible chemoprophylactic agent.

  11. ISOLATION OF HEPATIC OVAL CELLS FROM DIFFERENT MODEL RATS INCLUDING DIABETIC RATS

    Institute of Scientific and Technical Information of China (English)

    LU Ying-li; YE Ting-ting; XIA Fang-zhen; WANG Ning-jian; YANG Hua; CHEN Yi

    2009-01-01

    Objective To acquire oval cells (progenitor stem cells) from adult rat liver of different models including diabetic rats. Methods Thirty Sprague-Dawley (SD) rats were divided into 5 groups randomly: control, 2-acetylaminofluorene (2-AAF), 2-AAF+partial hepatectomy (PH), 2-AAF+carbon tetrachloride (CCl4), and diabetic groups. As two-step collagenase perfusion protocol of Seglen, oval cells were isolated by Percoll density gradient centrifugation. Thy1.1 positive cells were sorted by flow cytometry, and then cultured in Dulbeccos minimum Eagles medium (DMEM). Immunofluorescence staining was applied to labelling Thy1.1. Results Different rates of Thy1.1 positive oval cells were found in different rat model groups: 0.5% in 2-AAF, 0.3% in 2-hAAF+PH, 0.2% in 2-AAF+CCl4 , 0.1% in diabetic, and 0.0% in control. Isolated cells adhered to plate with fusiform or polygon as epithelial cells. Conclusion Progenitor stem cells exist in injured liver tissue including those from diabetic rats.

  12. Alcohol-Adapted Anger Management Treatment: A Randomized Controlled Trial of an Innovative Therapy for Alcohol Dependence.

    Science.gov (United States)

    Walitzer, Kimberly S; Deffenbacher, Jerry L; Shyhalla, Kathleen

    2015-12-01

    A randomized controlled trial for an innovative alcohol-adapted anger management treatment (AM) for outpatient alcohol dependent individuals scoring moderate or above on anger is described. AM treatment outcomes were compared to those of an empirically-supported intervention, Alcoholics Anonymous Facilitation treatment (AAF). Clients in AM, relative to clients in AAF, were hypothesized to have greater improvement in anger and anger-related cognitions and lesser AA involvement during the 6-month follow-up. Anger-related variables were hypothesized to be stronger predictors of improved alcohol outcomes in the AM treatment condition and AA involvement was hypothesized to be a stronger predictor of alcohol outcomes in the AAF treatment group. Seventy-six alcohol dependent men and women were randomly assigned to treatment condition and followed for 6 months after treatment end. Both AM and AAF treatments were followed by significant reductions in heavy drinking days, alcohol consequences, anger, and maladaptive anger-related thoughts and increases in abstinence and self-confidence regarding not drinking to anger-related triggers. Treatment with AAF was associated with greater AA involvement relative to treatment with AM. Changes in anger and AA involvement were predictive of posttreatment alcohol outcomes for both treatments. Change in trait anger was a stronger predictor of posttreatment alcohol consequences for AM than for AAF clients; during-treatment AA meeting attendance was a stronger predictor of posttreatment heavy drinking and alcohol consequences for AAF than for AM clients. Anger-related constructs and drinking triggers should be foci in treatment of alcohol dependence for anger-involved clients.

  13. Cost-effectiveness of using an extensively hydrolyzed casein formula plus the probiotic Lactobacillus rhamnosus GG compared to an extensively hydrolyzed formula alone or an amino acid formula as first-line dietary management for cow’s milk allergy in the US

    Directory of Open Access Journals (Sweden)

    Ovcinnikova O

    2015-02-01

    Full Text Available Olga Ovcinnikova,1 Monica Panca,1 Julian F Guest1,2 1CATALYST Health Economics Consultants, Northwood, London, 2Faculty of Life Sciences and Medicine, King’s College, London, UK Objectives: The aim was to estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (eHCF plus the probiotic Lactobacillus rhamnosus GG (eHCF + LGG; Nutramigen LGG compared to an eHCF alone (Nutramigen and an amino acid formula (AAF; Neocate as first-line dietary management for cow’s milk allergy (CMA in the US. Methods: Using a cohort study design, the analysis was based on the case records of 136 eHCF-fed, 59 eHCF + LGG-fed, and 217 matched AAF-fed infants extracted from the Truven Health MarketScan® Commercial Claims Database (a nationally representative database of the commercially insured population of the US. Clinical outcomes and health care resource use (with corresponding costs at 2012 prices, following first-line dietary management with each formula, were estimated over 12 months from the start of feeding. Differences in infants’ outcomes and resource use between groups were adjusted for any differences in baseline covariates. Results: Infants were <6 months of age at presentation. Fifty-six percent of eHCF + LGG-fed infants were estimated to have been successfully managed by 9 months compared to 38% of eHCF-fed infants and 35% of AAF-fed infants (P<0.05 and P=0.003 respectively. Infants in the AAF group used significantly more health care resources and prescribed drugs than infants in the other two groups. The estimated cost of managing a CMA infant over the first 12 months following the start of feeding was $3,577, $3,781, and $6,255 for an eHCF + LGG-fed, eHCF-fed, and AAF-fed infant, respectively. Parents’ costs accounted for up to 10% of the total costs and the remainder was incurred by insurers. The analyses were robust to plausible changes in all variables. Conclusion: Using real world evidence, initial dietary

  14. Binding of aromatic amines to the rat hepatic Ah receptor in vitro and in vivo and the 8S and 4S estrogen receptor of rat uterus and rat liver

    Energy Technology Data Exchange (ETDEWEB)

    Cikryt, P.; Kaiser, T.; Gottlicher, M. (Univ. of Wuerzburg (West Germany))

    1990-08-01

    Studies on structurally related aromatic amines with different carcinogenic properties have shown that 2-acetylaminofluorene (2-AAF) and 2-acetylaminophenanthrene (AAP) inhibit the binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the Ah receptor in vitro. The apparent inhibitor constants (K{sub i}) are 2.3 {mu}M for 2-AAF and 2.7 {mu}M for AAP. In contrast, 4-acetylaminofluorene, an isomer of 2-AAF, and trans-4-acetylaminostilbene do not bind to the rat hepatic cytosolic Ah receptor. Pretreating female Wistar rats with 2-AAF or AAP leads to the induction of the P-450 isoenzymes that are under the control of the Ah receptor. Ornithine decarboxylase activity is induced by all aromatic amines tested irrespective of their Ah receptor affinity. The aromatic amines used as model compounds do not inhibit the binding of 17-{beta}-estradiol to the 8S and 4S estrogen receptor of rat uterus or rat liver in a competition assay analyzed using sucrose density gradient centrifugation. On the other hand, the aromatic amines bind to varying extents to another estrogen-binding protein of rat liver whose function and identity is still unknown. The study demonstrates that structurally related aromatic amines in their unmetabolized form interact differentially with a cellular target protein, the Ah receptor, in vitro as well as in vivo. However, a relationship between these effects and the postulated promoting properties of 2-AAF remains to be established.

  15. Participation of integrin α5β1 in the fibronectin-mediated adherence of enteroaggregative Escherichia coli to intestinal cells.

    Science.gov (United States)

    Izquierdo, Mariana; Alvestegui, Alejandra; Nataro, James P; Ruiz-Perez, Fernando; Farfan, Mauricio J

    2014-01-01

    Adherence to the intestinal epithelia is a key feature in enteroaggregative Escherichia coli (EAEC) infection. The aggregative adherence fimbriae (AAFs) are involved in EAEC interaction with receptors at the surface of intestinal cells. We and others have demonstrated that fibronectin is a receptor for AAF/II fimbriae. Considering that the major cellular receptor of fibronectin is integrin α5β1, in this study we evaluated the participation of this receptor in the fibronectin-mediated adherence of EAEC strain 042 to intestinal cells. We found that EAEC strain 042 has the ability to bind directly and indirectly to integrin α5β1; direct binding was not mediated by AAF/II fimbriae and indirect binding was mediated by AAF/II and fibronectin. Coimmunoprecipitation assays confirmed the formation of the complex AafA/fibronectin/integrin α5β1. To evaluate EAEC adherence to intestinal cells, T84 cells were incubated with fibronectin and an antibody that blocks the interaction region of integrin α5β1 to fibronectin, the RGD site. Under these conditions, we found the number of adherent bacteria to epithelial cells significantly reduced. Additionally, fibronectin-mediated adherence of EAEC strain 042 was abolished in HEp-2 cells transfected with integrin α5 shRNA. Altogether, our data support the involvement of integrin α5β1 in the fibronectin-mediated EAEC binding to intestinal cells.

  16. Participation of Integrin α5β1 in the Fibronectin-Mediated Adherence of Enteroaggregative Escherichia coli to Intestinal Cells

    Directory of Open Access Journals (Sweden)

    Mariana Izquierdo

    2014-01-01

    Full Text Available Adherence to the intestinal epithelia is a key feature in enteroaggregative Escherichia coli (EAEC infection. The aggregative adherence fimbriae (AAFs are involved in EAEC interaction with receptors at the surface of intestinal cells. We and others have demonstrated that fibronectin is a receptor for AAF/II fimbriae. Considering that the major cellular receptor of fibronectin is integrin α5β1, in this study we evaluated the participation of this receptor in the fibronectin-mediated adherence of EAEC strain 042 to intestinal cells. We found that EAEC strain 042 has the ability to bind directly and indirectly to integrin α5β1; direct binding was not mediated by AAF/II fimbriae and indirect binding was mediated by AAF/II and fibronectin. Coimmunoprecipitation assays confirmed the formation of the complex AafA/fibronectin/integrin α5β1. To evaluate EAEC adherence to intestinal cells, T84 cells were incubated with fibronectin and an antibody that blocks the interaction region of integrin α5β1 to fibronectin, the RGD site. Under these conditions, we found the number of adherent bacteria to epithelial cells significantly reduced. Additionally, fibronectin-mediated adherence of EAEC strain 042 was abolished in HEp-2 cells transfected with integrin α5 shRNA. Altogether, our data support the involvement of integrin α5β1 in the fibronectin-mediated EAEC binding to intestinal cells.

  17. Dynamics and diversity of microbial community succession in traditional fermentation of Shanxi aged vinegar.

    Science.gov (United States)

    Nie, Zhiqiang; Zheng, Yu; Du, Hongfu; Xie, Sankuan; Wang, Min

    2015-05-01

    The traditional fermentation of Shanxi aged vinegar (SAV), a well-known traditional Chinese vinegar, generally involves the preparation of starter daqu, starch saccharification, alcoholic fermentation (AF) and acetic acid fermentation (AAF). Dynamics and diversity of microbial community succession in daqu and other fermentation stages were investigated by denaturing gradient gel electrophoresis (DGGE). Results showed that eight bacterial genera and four fungal genera were found in daqu. However, Staphylococcus, Saccharopolyspora, Bacillus, Oceanobacillus, Enterobacter, Streptomyces, Eurotium, Monascus and Pichia in daqu were eradicated during AF. Four bacterial genera and three fungal genera were found in this stage. Weissella, Lactobacillus, Streptococcus, Saccharomyces, and Saccharomycopsis were the dominant microorganisms in the late stage of AF. During AAF, four bacterial genera and four fungal genera were found. Weissella, Streptococcus, Klebsiella, Escherichia, and Cladosporium gradually disappeared; the dominant microorganisms were Acetobacter, Lactobacillus, Saccharomycopsis, and Alternaria in the late stage of AAF. Alpha diversity metrics showed that fungal diversity in daqu was greater than that in AF and AAF. By contrast, bacterial diversity decreased from daqu to AF and increased in the first three days of AAF and then decreased. Hence, these results could help understand dynamics of microbial community succession in continuous fermentation of traditional Chinese vinegars.

  18. Performance analysis of acceleration resolution for radar signal

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Hongzhong; (赵宏钟); FU; Qiang; (付; 强)

    2003-01-01

    The high acceleration of moving targets has brought severe problems in radar signal processing, such as the decrease in output signal-noise-ratio and the deterioration of Doppler resolution. This paper presents an acceleration ambiguity function (AAF) for characterizing the acceleration effects and the acceleration resolution property in radar signal processing. The definition of the acceleration resolution based on AAF is also presented. Using AAF as an analyzing tool, some factors are derived including the loss factor of output SNR, the broadening factor of Doppler resolution, and the optimal accumulative time (OPT) caused by acceleration in linear-phase matched filtering. The convergent property of quadratic-phase matched-filter for searching for and estimating the acceleration is discussed. The results and conclusions are helpful for the quantitative analysis of the acceleration effects on signal processing, and for evaluation of the performance of acceleration in radar signal waveform design.

  19. Synthesis, characterization and antimicrobial studies of new bispyrazolines linked via 3-aryl ring with aliphatic chains

    Science.gov (United States)

    Yusuf, Mohamad; Jain, Payal

    2012-10-01

    The bispyrazolines 4a(a'-f') and 4b(a'-f') built around the aliphatic chains of varying lengths have been prepared by refluxing bischalcones 3a(a'-f') and 3b(a'-f') with phenyl hydrazine in alcoholic medium. The reactions of chalcones 2a and 2b with suitable 1,ω-dibromoalkanes in the presence of anhydrous K2CO3/dry acetone and Bu4N+I- (PTC) provided 3a(a'-f') and 3b(a'-f'), respectively. The antibacterial and antifungal activities of the synthesized compounds were evaluated against five bacterial and four fungal strains. The compounds 3ba', 3bc', 3bd', 3be', 3af', 4aa' and 4ba' showed better MIC (μg/mL) against the tested microorganisms.

  20. Comparison of occipitocervical and atlantoaxial fusion in treatment of unstable Jefferson fractures

    Directory of Open Access Journals (Sweden)

    Yong Hu

    2017-01-01

    Full Text Available Background: Controversy exists regarding the management of unstable Jefferson fractures, with some surgeons performing reduction and immobilization of the patient in a halo vest and others performing open reduction and internal fixation. This study compares the clinical and radiological outcome parameters between posterior atlantoaxial fusion (AAF and occipitocervical fusion (OCF constructs in the treatment of the unstable atlas fracture. Materials and Methods: 68 consecutive patients with unstable Jefferson fractures treated by AAF or OCF between October 2004 and March 2011 were included in this retrospective evaluation from institutional databases. The authors reviewed medical records and original images. The patients were divided into two surgical groups treated with either AAF ( n = 48, F/M 30:18 and OCF ( n = 20, F/M 13:7 fusion. Blood loss, operative time, Japanese Orthopaedic Association (JOA score, visual analog scale (VAS score, atlanto-dens interval, lateral mass displacement, complications, and the bone fusion rates were recorded. Results: Five patients with incomplete paralysis (7.4% demonstrated postoperative improvement by more than 1 grade on the American Spinal Injury Association impairment scale. The JOA score of the AAF group improved from 12.5 ± 3.6 preoperatively to 15.7 ± 2.3 postoperatively, while the JOA score of the OCF group improved from 11.2 ± 3.3 preoperatively to 14.8 ± 4.2 postoperatively. The VAS score of AAF group decreased from 4.8 ± 1.5 preoperatively to 1.0 ± 0.4 postoperatively, the VAS score of the OCF group decreased from 5.4 ± 2.2 preoperatively to 1.3 ± 0.9 postoperatively. Conclusions: The OCF or AAF combined with short-term external immobilization can establish the upper cervical stability and prevent further spinal cord injury and nerve function damage.

  1. Comparison of occipitocervical and atlantoaxial fusion in treatment of unstable Jefferson fractures

    Science.gov (United States)

    Hu, Yong; Yuan, Zhen-shan; Kepler, Christopher K; Dong, Wei-xin; Sun, Xiao-yang; Zhang, Jiao

    2017-01-01

    Background: Controversy exists regarding the management of unstable Jefferson fractures, with some surgeons performing reduction and immobilization of the patient in a halo vest and others performing open reduction and internal fixation. This study compares the clinical and radiological outcome parameters between posterior atlantoaxial fusion (AAF) and occipitocervical fusion (OCF) constructs in the treatment of the unstable atlas fracture. Materials and Methods: 68 consecutive patients with unstable Jefferson fractures treated by AAF or OCF between October 2004 and March 2011 were included in this retrospective evaluation from institutional databases. The authors reviewed medical records and original images. The patients were divided into two surgical groups treated with either AAF (n = 48, F/M 30:18) and OCF (n = 20, F/M 13:7) fusion. Blood loss, operative time, Japanese Orthopaedic Association (JOA) score, visual analog scale (VAS) score, atlanto-dens interval, lateral mass displacement, complications, and the bone fusion rates were recorded. Results: Five patients with incomplete paralysis (7.4%) demonstrated postoperative improvement by more than 1 grade on the American Spinal Injury Association impairment scale. The JOA score of the AAF group improved from 12.5 ± 3.6 preoperatively to 15.7 ± 2.3 postoperatively, while the JOA score of the OCF group improved from 11.2 ± 3.3 preoperatively to 14.8 ± 4.2 postoperatively. The VAS score of AAF group decreased from 4.8 ± 1.5 preoperatively to 1.0 ± 0.4 postoperatively, the VAS score of the OCF group decreased from 5.4 ± 2.2 preoperatively to 1.3 ± 0.9 postoperatively. Conclusions: The OCF or AAF combined with short-term external immobilization can establish the upper cervical stability and prevent further spinal cord injury and nerve function damage. PMID:28216748

  2. Measurement Capabilities of the DOE ARM Aerial Facility

    Science.gov (United States)

    Schmid, B.; Tomlinson, J. M.; Hubbe, J.; Comstock, J. M.; Kluzek, C. D.; Chand, D.; Pekour, M. S.

    2012-12-01

    The Department of Energy Atmospheric Radiation Measurement (ARM) Program is a climate research user facility operating stationary ground sites in three important climatic regimes that provide long-term measurements of climate relevant properties. ARM also operates mobile ground- and ship-based facilities to conduct shorter field campaigns (6-12 months) to investigate understudied climate regimes around the globe. Finally, airborne observations by ARM's Aerial Facility (AAF) enhance the surface-based ARM measurements by providing high-resolution in situ measurements for process understanding, retrieval algorithm development, and model evaluation that is not possible using ground-based techniques. AAF started out in 2007 as a "virtual hangar" with no dedicated aircraft and only a small number of instruments owned by ARM. In this mode, AAF successfully carried out several missions contracting with organizations and investigators who provided their research aircraft and instrumentation. In 2009, the Battelle owned G-1 aircraft was included in the ARM facility. The G-1 is a large twin turboprop aircraft, capable of measurements up to altitudes of 7.5 km and a range of 2,800 kilometers. Furthermore the American Recovery and Reinvestment Act of 2009 provided funding for the procurement of seventeen new instruments to be used aboard the G-1 and other AAF virtual-hangar aircraft. AAF now executes missions in the virtual- and real-hangar mode producing freely available datasets for studying aerosol, cloud, and radiative processes in the atmosphere. AAF is also heavily engaged in the maturation and testing of newly developed airborne sensors to help foster the next generation of airborne instruments. In the presentation we will showcase science applications based on measurements from recent field campaigns such as CARES, CALWATER and TCAP.

  3. Financing Nonappropriated Fund (NAF) Major Construction.

    Science.gov (United States)

    1985-03-09

    include Greyhound; Woolworth ; Howard Johnson; and the Army, Air Force Exchange Service (AAFES). The AAFES contract is for 185 units to be developed...J. W. Morris, Ltd ., from Delta Research Corp as a supplement to Delta’s Final Report, entitled Financing lonappropriated Fund, Major Construction...nofsso . - .iao 90 .oen .omo Ko er Ki Mot-.*. j. 1U.%c fto HOI..’ Z. J.Hl SENNETT. JR. Mdaf t 0ing April 12, 1985 Mr. Norman Howard J. W. Morris, Ltd

  4. Replacing a piece of loop-structure in the substrate-binding groove of Aspergillus usamii β-mannanase, AuMan5A, to improve its enzymatic properties by rational design.

    Science.gov (United States)

    Dong, Yun Hai; Li, Jian Fang; Hu, Die; Yin, Xin; Wang, Chun Juan; Tang, Shi Han; Wu, Min Chen

    2016-05-01

    To perfect the enzymatic properties of AuMan5A, a mesophilic glycoside hydrolase (GH) family 5 β-mannanase from Aspergillus usamii, its loop-structure substitution was carried out by rational design and followed by megaprimer PCR. Based on the structural analysis and enzymatic property comparison of various β-mannanases, a piece of loop-structure with seven amino acids between two β-strands (βD and βE) in the substrate-binding groove, named "Loop DE," was speculated to be correlative to the thermostability and catalytic efficiency of GH family 5 β-mannanases. Therefore, three AuMan5A's mutants, AuMan5A-Af, AuMan5A-An, and AuMan5A-Th, were designed by substituting a Loop DE sequence ((316)KSPDGGN(322)) of AuMan5A with the corresponding sequences of other three family 5 β-mannanases, respectively. Then, the mutant-encoding genes, Auman5A-Af, Auman5A-An, and Auman5A-Th, were constructed as designed theoretically and then expressed in Pichia pastoris GS115. The expressed recombinant AuMan5A-Af (re-AuMan5A-Af) displayed the temperature optimum (T opt) of 75 °C, T m value of 76.6 °C and half-life (t 1/2) of 480 min at 70 °C, which were 10 and 12.1 °C higher and 48-fold longer than those of re-AuMan5A, respectively. Its catalytic efficiency (k cat/K m) was 12.7-fold that of re-AuMan5A. What is more, the site-directed mutagenesis of D320G in AuMan5A-Af was performed. The T opt and t 1/2 of expressed re-AuMan5A-Af(D320G) decreased to 70 °C and 40 min, respectively, while its k cat/K m was only 35 % of that of re-AuMan5A-Af. These results demonstrated that the mutation of G320 (in AuMan5A) into D320 (in AuMan5A-Af) through Loop DE substitution was mainly responsible for the thermostability and catalytic efficiency improvement of AuMan5A-Af.

  5. Anticuerpos antifosfolípido en el lupus eritematoso sistémico

    OpenAIRE

    López Soto, Alfonso

    1990-01-01

    INTRODUCCION: El lupus eritematoso sistémico (LES) es una enfermedad autoinmune que se caracteriza por la producción de numerosos autoanticuerpos. Recientemente, se ha descrito un nuevo grupo denominado anticuerpos antifosfolípido (AAF), cuyo estudio ha despertado un gran interés por sus implicaciones clínicas y patogenéticas. Los AAF son un grupo heterogéneo de autoanticuerpos adquiridos de forma espontánea, que van dirigidos contra estructuras fosfolipídicas de las membranas celulares.La de...

  6. System Engineering Analysis of Squadron Officer College

    Science.gov (United States)

    2012-03-01

    intended to build upon previous levels. “PME conveys the broad body of knowledge and develops the habits of mind essential to the military professional’s...students thru the Army and Air Force Exchange Service (AAFES) snack bar. Managers are responsible for ensuring adequate support throughout the

  7. EST Table: BP121297 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BP121297 ceN-4665 10/09/28 44 %/215 aa ref|XP_001649323.1| n-acetylgalactosaminyltr...actosaminyltransferase#status:Confirmed#UniProt :O61397#protein_id:AAF60620.1 10/09/10 41 %/212

  8. 77 FR 8869 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License

    Science.gov (United States)

    2012-02-15

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change the Community of License AGENCY: Federal... the community of license: BBC BROADCASTING, INC, Station KPRI, Facility ID 21416, BP-20090226AAF, From..., BMPED-20120131ALI, From RIDGEVILLE, SC, To ST. GEORGE, SC; RADIO LICENSE HOLDING II, LLC, Station...

  9. NCBI nr-aa BLAST: CBRC-TGUT-05-0042 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-05-0042 ref|NP_001076121.1| potassium voltage-gated channel, delayed-rect...bunit 3) gb|AAF22833.1|AF209723_1 voltage-gated delayed-rectifier potassium channel Kv9.3 [Oryctolagus cuniculus] NP_001076121.1 0.0 85% ...

  10. NCBI nr-aa BLAST: CBRC-FRUB-02-0169 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0169 ref|NP_001076121.1| potassium voltage-gated channel, delayed-rect...bunit 3) gb|AAF22833.1|AF209723_1 voltage-gated delayed-rectifier potassium channel Kv9.3 [Oryctolagus cuniculus] NP_001076121.1 1e-134 55% ...

  11. NCBI nr-aa BLAST: CBRC-OGAR-01-0901 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OGAR-01-0901 ref|NP_001076121.1| potassium voltage-gated channel, delayed-rect...bunit 3) gb|AAF22833.1|AF209723_1 voltage-gated delayed-rectifier potassium channel Kv9.3 [Oryctolagus cuniculus] NP_001076121.1 1e-172 95% ...

  12. NCBI nr-aa BLAST: CBRC-ETEL-01-0884 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-0884 ref|NP_001076121.1| potassium voltage-gated channel, delayed-rect...bunit 3) gb|AAF22833.1|AF209723_1 voltage-gated delayed-rectifier potassium channel Kv9.3 [Oryctolagus cuniculus] NP_001076121.1 0.0 92% ...

  13. NCBI nr-aa BLAST: CBRC-EEUR-01-1046 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-1046 ref|NP_001076121.1| potassium voltage-gated channel, delayed-rect...bunit 3) gb|AAF22833.1|AF209723_1 voltage-gated delayed-rectifier potassium channel Kv9.3 [Oryctolagus cuniculus] NP_001076121.1 0.0 91% ...

  14. FAA (Federal Aviation Administration) Aviation Forecasts, Fiscal Years 1990-2001

    Science.gov (United States)

    1990-03-01

    Chico, CA (CIC) San Luis Obispo, CA (SBP) Chino, CA (CNO) Santa Ana, CA (SNA) Concord, CA (CCR) Santa Barbara, CA (SBA) El Monte, CA (EMT) Santa Maria ...San Juan International, PR (SJU) San Antonio Stinson, TX (SSF) San Juan Isla Grande, PR (SIG) Tomball D. W. Hooks, TX (DWH) Kwajalein AAF, WK (KWA

  15. FAA Aviation Forecasts, Fiscal Years 1994-2005

    Science.gov (United States)

    1994-03-01

    Concord, CA (CCR) Santa Barbara, CA (SBA) El Monte, CA (EMT) Santa Maria Public, CA (SMX) H-1 FAA TOWERED AIRPORTS Santa Monica, CA (SMO) Sanford, Fl...Juan Isla Grande, PR (SIG) Kwajalein AAF, WK (KWA) Pago Pago International, AS (TUT) H-6 APPENDIX I CONTRACT TOWERS 1. Flagstaff, Arizona (FLG) 2

  16. Influence of nucleotide excision repair on N-hydroxy-2-acetylaminofluorene-induced mutagenesis studied in λlacZ-transgenic mice

    NARCIS (Netherlands)

    Frijhoff, A.F.W.; Krul, C.A.M.; Vries, A. de; Kelders, M.C.J.M.; Weeda, G.; Steeg, H. van; Baan, R.A.

    1998-01-01

    To study the influence of nucleotide excision repair (NER) on mutagenesis in vivo, ERCC1+/-, XPA-/-, and wild-type (ERCC1+/+ and XPA+/+, respectively) λlacZ-transgenic mice were treated i.p. with N-hydroxy-2-acetylaminofluorene (N-OH-AAF) and lacZ mutant frequencies were determined in liver. No sign

  17. 78 FR 33019 - Proposed Amendment of Class E Airspace; Commerce, TX

    Science.gov (United States)

    2013-06-03

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Commerce, TX AGENCY... action proposes to amend Class E airspace at Commerce, TX. Additional controlled airspace is necessary to accommodate new Standard Instrument Approach Procedures (SIAPs) at Commerce Municipal Airport (AAF). The...

  18. Hepatoprotective Potential of Some Local Medicinal Plants against 2-Acetylaminoflourene-Induced Damage in Rat

    Science.gov (United States)

    Adetutu, Adewale; Olorunnisola, Olubukola S.

    2013-01-01

    The in vivo micronucleus assay was used to examine the anticlastogenic effects of crude extracts of Bridelia ferruginea, Vernonia amygdalina, Tridax procumbens, Ocimum gratissimum, and Lawsonia inermis in Wistar albino rats. Extracts of doses of 100 mg/kg body weight were given to rats in five groups for seven consecutive days followed by a single dose of 2-AAF (0.5 mmol/kg body weight). The rats were sacrificed after 24 hours and their bone marrow smears were prepared on glass slides stained with Giemsa. The micronucleated polychromatic erythrocyte cells (mPCEs) were thereafter recorded. The hepatoprotective effects of the plant extracts against 2-AAF-induced liver toxicity in rats were evaluated by monitoring the levels of alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), and histopathological analysis. The results of the 2-AAF-induced liver toxicity experiments showed that rats treated with the plant extracts (100 mg/kg) showed a significant decrease in mPCEs as compared with the positive control. The rats treated with the plant extracts did not show any significant change in the concentration of ALP and GGT in comparison with the negative control group whereas the 2-AAF group showed a significant increase (P extracts also showed protective effects against histopathological alterations. This study suggests that the leaf extracts have hepatoprotective potential, thereby justifying their ethnopharmacological uses. PMID:24163694

  19. NCBI nr-aa BLAST: CBRC-AGAM-04-0125 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-04-0125 ref|NP_525103.2| bunched CG5461-PA, isoform A [Drosophila melanog...aster] sp|Q24523|BUN2_DROME Protein bunched, class 2 isoform (Protein shortsighted) gb|AAF53201.2| CG5461-PA, isoform A [Drosophila melanogaster] NP_525103.2 6e-62 37% ...

  20. NCBI nr-aa BLAST: CBRC-AGAM-01-0058 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-01-0058 ref|NP_525103.2| bunched CG5461-PA, isoform A [Drosophila melanog...aster] sp|Q24523|BUN2_DROME Protein bunched, class 2 isoform (Protein shortsighted) gb|AAF53201.2| CG5461-PA, isoform A [Drosophila melanogaster] NP_525103.2 2e-14 32% ...

  1. NCBI nr-aa BLAST: CBRC-DSIM-03-0004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-03-0004 ref|NP_274421.1| hypothetical protein NMB1408 [Neisseria meningit...idis MC58] gb|AAF41770.1| hypothetical protein NMB1408 [Neisseria meningitidis MC58] NP_274421.1 0.62 21% ...

  2. NCBI nr-aa BLAST: CBRC-LAFR-01-1399 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1399 ref|NP_274062.1| hypothetical protein NMB1028 [Neisseria meningit...idis MC58] gb|AAF41427.1| conserved hypothetical protein [Neisseria meningitidis MC58] NP_274062.1 1.9 31% ...

  3. Stuttering in Adults: The Acoustic Startle Response, Temperamental Traits, and Biological Factors

    Science.gov (United States)

    Alm, Per A.; Risberg, Jarl

    2007-01-01

    The purpose of this study was to investigate the relation between stuttering and a range of variables of possible relevance, with the main focus on neuromuscular reactivity, and anxiety. The explorative analysis also included temperament, biochemical variables, heredity, preonset lesions, and altered auditory feedback (AAF). An increased level of…

  4. InterProScan Result: AU006297 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available AU006297 AU006297_3_ORF1 AAF0FB40F5602A55 PANTHER PTHR12898 THYROID HORMONE RECEPTO...R-ASSOCIATED PROTEIN COMPLEX 100 KDA COMPONENT (TRAP100) (THYROID HORMONE RECEPTOR ASSOCIATED PROTEIN 4) 1.4e-39 T IPR021429 unintegrated ...

  5. Tarnished Victory: Divided Command in the Pacific and its Consequences in the Naval Battle for Leyte Gulf

    Science.gov (United States)

    2009-06-12

    requests for help. Halsey was unaware how 46 significantly MacArthur’s edict that all Seventh Fleet message traffic pass through Manus degraded...75, USSBS No. 378, 1945. http://ibiblio.org/hyperwar/AAF/USSBS/IJO/IJO-75.html. 16 June 2009. Vego, Milan . The Battle for Leyte, 1944: Allied and

  6. 34 CFR 464.42 - What limit applies to purchasing computer hardware and software?

    Science.gov (United States)

    2010-07-01

    ... computer hardware and software? Not more than ten percent of funds received under any grant under this part may be used to purchase computer hardware or software. (Authority: 20 U.S.C. 1208aa(f)) ... 34 Education 3 2010-07-01 2010-07-01 false What limit applies to purchasing computer hardware...

  7. Analysis of Bacterial Diversity During Acetic Acid Fermentation of Tianjin Duliu Aged Vinegar by 454 Pyrosequencing.

    Science.gov (United States)

    Peng, Qian; Yang, Yanping; Guo, Yanyun; Han, Ye

    2015-08-01

    The vinegar pei harbors complex bacterial communities. Prior studies revealing the bacterial diversity involved were mainly conducted by culture-dependent methods and PCR-DGGE. In this study, 454 pyrosequencing was used to investigate the bacterial communities in vinegar pei during the acetic acid fermentation (AAF) of Tianjin Duliu aged vinegar (TDAV). The results showed that there were 7 phyla and 24 families existing in the vinegar pei, with 2 phyla (Firmicutes, Protebacteria) and 4 families (Lactobacillaceae, Acetobacteracae, Enterobacteriaceae, Chloroplast) predominating. The genus-level identification revealed that 9 genera were the relatively stable, consistent components in different stages of AAF, including the most abundant genus Lactobacillus followed by Acetobacter and Serratia. Additionally, the bacterial community in the early fermentation stage was more complex than those in the later stages, indicating that the accumulation of organic acids provided an appropriate environment to filter unwanted bacteria and to accelerate the growth of required ones. This study provided basic information of bacterial patterns in vinegar pei and relevant changes during AAF of TDAV, and could be used as references in the following study on the implementation of starter culture as well as the improvement of AAF process.

  8. Numbers Matter: Post-2014 Afghan National Security Force End Strength

    Science.gov (United States)

    2013-03-01

    Afghan Border Police ( ABP ), and the Afghan National Civil Order Police (ANCOP).16 Two other Afghan security organizations, which did not factor...Air Force (AAF), Afghan Border Police ( ABP ), and Afghan Uniform Police (AUP), is also a significant challenge to the legitimacy of the ANSF with the

  9. Summary of Independent Assessment of the Afghan National Security Forces

    Science.gov (United States)

    2014-02-01

    Afghan National Police (ANP), which are the Afghan Border Police ( ABP ), Afghan National Civil Order Police (ANCOP), Afghan Uniform Police (AUP...intentionally left blank 43 Acronyms AACP Afghan Anti-Crime Police AAF Afghan Air Force ABP Afghan Border Police ALP Afghan

  10. Gene : CBRC-DSIM-04-0021 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-04-0021 3R A Opsins OPS1_DROME 0.0 98% ref|NP_524407.1| neither inactivat...toreceptor cells opsin) (Neither inactivation nor afterpotential E protein) gb|AAA28733.1| opsin gb|AAF55712

  11. Cockayne syndrome: varied requirement of transcription-coupled nucleotide excision repair for the removal of three structurally different adducts from transcribed DNA.

    Directory of Open Access Journals (Sweden)

    Nataliya Kitsera

    Full Text Available Hereditary defects in the transcription-coupled nucleotide excision repair (TC-NER pathway of damaged DNA cause severe neurodegenerative disease Cockayne syndrome (CS, however the origin and chemical nature of the underlying DNA damage had remained unknown. To find out, to which degree the structural properties of DNA lesions determine the extent of transcription arrest in human CS cells, we performed quantitative host cell reactivation analyses of expression vectors containing various synthetic adducts. We found that a single 3-(deoxyguanosin-N2-yl-2-acetylaminofluorene adduct (dG(N2-AAF constitutes an unsurmountable obstacle to transcription in both CS-A and CS-B cells and is removed exclusively by the CSA- and CSB-dependent pathway. In contrast, contribution of the CS proteins to the removal of two other transcription-blocking DNA lesions - N-(deoxyguanosin-8-yl-2-acetylaminofluorene (dG(C8-AAF and cyclobutane thymine-thymine (TT dimer - is only minor (TT dimer or none (dG(C8-AAF. The unique properties of dG(N2-AAF identify this adduct as a prototype for a new class of DNA lesions that escape the alternative global genome repair and could be critical for the CS pathogenesis.

  12. Auditory Masking Effects on Speech Fluency in Apraxia of Speech and Aphasia: Comparison to Altered Auditory Feedback

    Science.gov (United States)

    Jacks, Adam; Haley, Katarina L.

    2015-01-01

    Purpose: To study the effects of masked auditory feedback (MAF) on speech fluency in adults with aphasia and/or apraxia of speech (APH/AOS). We hypothesized that adults with AOS would increase speech fluency when speaking with noise. Altered auditory feedback (AAF; i.e., delayed/frequency-shifted feedback) was included as a control condition not…

  13. NCBI nr-aa BLAST: CBRC-CFAM-22-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-22-0005 ref|NP_058623.1| polycystic kidney disease 2-like 2 [Mus musculus...] sp|Q9JLG4|PK2L2_MOUSE Polycystic kidney disease 2-like 2 protein (Polycystin-L2) gb|AAF65621.1|AF182033_1 polycystic

  14. NCBI nr-aa BLAST: CBRC-FCAT-01-0217 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-0217 ref|NP_001027996.1| opioid receptor, mu 1 [Macaca mulatta] sp|Q9M...YW9|OPRM_MACMU Mu-type opioid receptor (MOR-1) gb|AAF97249.2|AF286024_1 mu opioid receptor [Macaca mulatta] NP_001027996.1 5e-35 87% ...

  15. NCBI nr-aa BLAST: CBRC-RMAC-04-0055 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-04-0055 ref|NP_001027996.1| opioid receptor, mu 1 [Macaca mulatta] sp|Q9M...YW9|OPRM_MACMU Mu-type opioid receptor (MOR-1) gb|AAF97249.2|AF286024_1 mu opioid receptor [Macaca mulatta] NP_001027996.1 0.0 99% ...

  16. NCBI nr-aa BLAST: CBRC-CREM-01-0003 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-0003 ref|NP_051147.1| NADH dehydrogenase subunit 2 [Cafeteria roenberg...ensis] gb|AAF05798.1|AF193903_21 NADH dehydrogenase subunit 2 [Cafeteria roenbergensis] NP_051147.1 1e-04 27% ...

  17. NCBI nr-aa BLAST: CBRC-TTRU-01-1294 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1294 ref|NP_051157.1| ATP synthase F0 subunit 6 [Cafeteria roenbergens...is] gb|AAF05808.1|AF193903_31 ATP synthase F0 subunit 6 [Cafeteria roenbergensis] NP_051157.1 0.057 29% ...

  18. NCBI nr-aa BLAST: CBRC-DNOV-01-0972 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0972 ref|NP_051157.1| ATP synthase F0 subunit 6 [Cafeteria roenbergens...is] gb|AAF05808.1|AF193903_31 ATP synthase F0 subunit 6 [Cafeteria roenbergensis] NP_051157.1 0.001 29% ...

  19. NCBI nr-aa BLAST: CBRC-DNOV-01-2150 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2150 ref|NP_051152.1| ribosomal protein S4 [Cafeteria roenbergensis] g...b|AAF05803.1|AF193903_26 ribosomal protein S4 [Cafeteria roenbergensis] NP_051152.1 0.86 27% ...

  20. NCBI nr-aa BLAST: CBRC-DNOV-01-2064 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2064 ref|NP_051147.1| NADH dehydrogenase subunit 2 [Cafeteria roenberg...ensis] gb|AAF05798.1|AF193903_21 NADH dehydrogenase subunit 2 [Cafeteria roenbergensis] NP_051147.1 0.009 24% ...

  1. NCBI nr-aa BLAST: CBRC-CBRE-01-1180 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CBRE-01-1180 ref|NP_051147.1| NADH dehydrogenase subunit 2 [Cafeteria roenberg...ensis] gb|AAF05798.1|AF193903_21 NADH dehydrogenase subunit 2 [Cafeteria roenbergensis] NP_051147.1 2e-05 24% ...

  2. AcEST: BP918121 [AcEST

    Lifescience Database Archive (English)

    Full Text Available tr|Q9LS51|Q9LS51_ARATH Gb|AAF24608.1 (Putative uncharacterized p... 175 9e-43 tr|A7UDM4|A7UDM4_MAIZE CFM2 al...ternative polyadenylation form 2 (... 171 1e-41 tr|A7UDM2|A7UDM2_MAIZE CFM2 (Frag

  3. Dicty_cDB: Contig-U05675-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 799158 ) PPTI537TF Solanum tuberosum RHPOTKEY BAC ends Sol... 50 0.070 1 ( CU603906 ) Theobroma cacao, mRNA ...um berghei strain ANKA cDNA clone:MG01826... 44 4.3 1 ( CU595687 ) Theobroma cacao, mRNA sequence (KZ0AAF10Y

  4. NCBI nr-aa BLAST: CBRC-OCUN-01-0083 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-0083 ref|YP_665648.1| NADH dehydrogenase subunit 5 [Nephroselmis oliva...cea] gb|AAF03175.1|AF110138_7 NADH dehydrogenase subunit 5 [Nephroselmis olivacea] YP_665648.1 1e-81 47% ...

  5. NCBI nr-aa BLAST: CBRC-DDIS-10-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-10-0001 ref|YP_665678.1| ATP synthase F0 subunit 6 [Nephroselmis olivacea...] gb|AAF03205.1|AF110138_37 ATP synthase F0 subunit 6 [Nephroselmis olivacea] YP_665678.1 8e-46 44% ...

  6. NCBI nr-aa BLAST: CBRC-PTRO-21-0010 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-21-0010 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 1e-07 45% ...

  7. NCBI nr-aa BLAST: CBRC-RNOR-17-0004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-17-0004 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 5e-22 41% ...

  8. NCBI nr-aa BLAST: CBRC-DRER-05-0036 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DRER-05-0036 ref|NP_297473.1| hypothetical protein XF0180 [Xylella fastidiosa ...9a5c] gb|AAF82993.1|AE003872_4 hypothetical protein XF_0180 [Xylella fastidiosa 9a5c] NP_297473.1 2e-07 38% ...

  9. NCBI nr-aa BLAST: CBRC-DRER-26-0217 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DRER-26-0217 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 1e-29 50% ...

  10. NCBI nr-aa BLAST: CBRC-DRER-18-0003 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DRER-18-0003 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 3e-27 51% ...

  11. NCBI nr-aa BLAST: CBRC-PMAR-01-0517 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0517 ref|NP_297916.1| hypothetical protein XF0626 [Xylella fastidiosa ...9a5c] gb|AAF83436.1|AE003908_4 hypothetical protein XF_0626 [Xylella fastidiosa 9a5c] NP_297916.1 2e-22 50% ...

  12. NCBI nr-aa BLAST: CBRC-RMAC-06-0012 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-06-0012 ref|NP_297473.1| hypothetical protein XF0180 [Xylella fastidiosa ...9a5c] gb|AAF82993.1|AE003872_4 hypothetical protein XF_0180 [Xylella fastidiosa 9a5c] NP_297473.1 2e-09 34% ...

  13. NCBI nr-aa BLAST: CBRC-LAFR-01-2065 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-2065 ref|NP_297864.1| hypothetical protein XF0574 [Xylella fastidiosa ...9a5c] gb|AAF83384.1|AE003904_5 hypothetical protein XF_0574 [Xylella fastidiosa 9a5c] NP_297864.1 0.25 37% ...

  14. NCBI nr-aa BLAST: CBRC-RNOR-04-0033 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0033 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 1e-31 58% ...

  15. NCBI nr-aa BLAST: CBRC-OANA-01-2205 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-2205 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 5e-16 44% ...

  16. NCBI nr-aa BLAST: CBRC-CJAC-01-0495 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0495 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 7e-13 34% ...

  17. NCBI nr-aa BLAST: CBRC-XTRO-01-0119 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0119 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 0.039 32% ...

  18. NCBI nr-aa BLAST: CBRC-OLAT-12-0010 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-12-0010 ref|NP_298556.1| 1,4-beta-cellobiosidase [Xylella fastidiosa 9a5c...] gb|AAF84076.1|AE003960_10 1,4-beta-cellobiosidase [Xylella fastidiosa 9a5c] NP_298556.1 4e-17 52% ...

  19. NCBI nr-aa BLAST: CBRC-PMAR-01-0652 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0652 ref|NP_297916.1| hypothetical protein XF0626 [Xylella fastidiosa ...9a5c] gb|AAF83436.1|AE003908_4 hypothetical protein XF_0626 [Xylella fastidiosa 9a5c] NP_297916.1 5e-11 36% ...

  20. NCBI nr-aa BLAST: CBRC-TSYR-01-0731 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TSYR-01-0731 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 1e-14 50% ...

  1. NCBI nr-aa BLAST: CBRC-PHAM-01-1773 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-1773 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 1e-15 41% ...

  2. NCBI nr-aa BLAST: CBRC-RNOR-02-0049 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-02-0049 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 8e-21 48% ...

  3. NCBI nr-aa BLAST: CBRC-PHAM-01-1727 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-1727 ref|NP_298108.1| endo-1,4-beta-glucanase [Xylella fastidiosa 9a5c...] gb|AAF83628.1|AE003921_8 endo-1,4-beta-glucanase [Xylella fastidiosa 9a5c] NP_298108.1 3e-48 68% ...

  4. NCBI nr-aa BLAST: CBRC-RNOR-05-0256 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-05-0256 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 5e-20 48% ...

  5. NCBI nr-aa BLAST: CBRC-SARA-01-0590 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0590 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 8e-19 51% ...

  6. NCBI nr-aa BLAST: CBRC-FCAT-01-0356 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-0356 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 0.001 30% ...

  7. NCBI nr-aa BLAST: CBRC-PABE-17-0004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-17-0004 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 3e-14 42% ...

  8. NCBI nr-aa BLAST: CBRC-RMAC-20-0035 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-20-0035 ref|NP_297344.1| hypothetical protein XF0051 [Xylella fastidiosa ...9a5c] gb|AAF82864.1|AE003859_5 hypothetical protein XF_0051 [Xylella fastidiosa 9a5c] NP_297344.1 3e-20 51% ...

  9. Research data supporting "Photon recycling in lead-iodide perovskite solar cells"

    OpenAIRE

    Pazos-Outón, Luis M.; Szumilo, Monika; Lamboll, Robin; Richter, Johannes M.; Crespo-Quesada, Micaela; Abdi - Jalebi, Mojtaba; Beeson, Harry J.; Vrucinic, Milan; Alsari, Mejd; Snaith, Henry J.; Ehrler, Bruno; Friend, Richard H.; Deschler, Felix

    2016-01-01

    Data for the figures presented in the manuscript. These research data support “Photon recycling in lead-iodide perovskite solar cells” published in “Science” (http://dx.doi.org./10.1126/science.aaf1168) This work was supported by the EPSRC [grant number EP/M005143/1] and Winton Programme for the Physics of Sustainability.

  10. 32 CFR 552.152 - Prohibited practices.

    Science.gov (United States)

    2010-07-01

    ... wines, malt beverages and distilled spirits. (1) By military personnel in uniform during duty hours... by section II of AR 215-2, and at Army/Air Force Exchange Service (AAFES) eating establishments which...) Relationships between service members of different rank or sex which involve or reasonably give the...

  11. Gene : CBRC-CJAC-01-0709 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0709 UN A Chemokines and chemotactic factors receptors CCR1_HUMAN 0.0 ...90% gb|AAF36453.1|AF127528_1 chemokine receptor [Callithrix jacchus] 0.0 100% METSATTEDYDMITEFDYSDATPCHKANER

  12. Restrained Policy and Careless Execution: Allied Strategic Bombing on the Netherlands in the Second World War

    Science.gov (United States)

    2011-05-19

    Nederland 1919- 1945. (Series). Den Haag, The Netherlands: Instituut voor Nederlandse Geschiedenis, 2004. AAF Historical Division, United States Airforce...food and money to the crew of a downed British Wellington. Source: A. Korthals Altes, Luchtgevaar: Luchtaanvallen op Nederland , 1940- 1945...of the Liberation of Europe (New York: Free Press, 2008). 6Chris van der Heijden, Grijs Verleden: Nederland en de Tweede Wereldoorlog (Amsterdam

  13. NCBI nr-aa BLAST: CBRC-AGAM-01-0071 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-01-0071 ref|NP_476722.1| shotgun CG3722-PA [Drosophila melanogaster] sp|Q...24298|CADE_DROME DE-cadherin precursor (Protein shotgun) gb|AAF46659.1| CG3722-PA [Drosophila melanogaster] NP_476722.1 0.0 40% ...

  14. NCBI nr-aa BLAST: CBRC-AGAM-01-0070 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-01-0070 ref|NP_476722.1| shotgun CG3722-PA [Drosophila melanogaster] sp|Q...24298|CADE_DROME DE-cadherin precursor (Protein shotgun) gb|AAF46659.1| CG3722-PA [Drosophila melanogaster] NP_476722.1 0.0 42% ...

  15. An Analysis of Turnover Intentions: A Reexamination of Air Force Civil Engineering Company Grade Officers

    Science.gov (United States)

    2012-03-01

    Air Force Exchange Service (AAFES), shopping privileges at the commissary (military grocery store), and free access to gym facilities. Mitchell et al...of sex on intent and actual job changes. Human Relations, 46, 987-1006. Werbel, J. D., & Bedeian, A. G. (1989). Intended turnover as a function of

  16. NCBI nr-aa BLAST: CBRC-DDIS-02-0178 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-02-0178 sp|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprotein precursor [Contains: Denti...n phosphoprotein (Dentin phosphophoryn) (DPP); Dentin sialoprotein (DSP)] gb|AAF42472.1|AF163151_1 dentin sialophosphoprotein precursor [Homo sapiens] Q9NZW4 1e-119 32% ...

  17. NCBI nr-aa BLAST: CBRC-PMAR-01-0431 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0431 sp|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprotein precursor [Contains: Denti...n phosphoprotein (Dentin phosphophoryn) (DPP); Dentin sialoprotein (DSP)] gb|AAF42472.1|AF163151_1 dentin sialophosphoprotein precursor [Homo sapiens] Q9NZW4 1e-157 47% ...

  18. NCBI nr-aa BLAST: CBRC-HSAP-04-0040 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-04-0040 sp|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprotein precursor [Contains: Denti...n phosphoprotein (Dentin phosphophoryn) (DPP); Dentin sialoprotein (DSP)] gb|AAF42472.1|AF163151_1 dentin sialophosphoprotein precursor [Homo sapiens] Q9NZW4 0.0 93% ...

  19. NCBI nr-aa BLAST: CBRC-RMAC-05-0016 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-05-0016 sp|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprotein precursor [Contains: Denti...n phosphoprotein (Dentin phosphophoryn) (DPP); Dentin sialoprotein (DSP)] gb|AAF42472.1|AF163151_1 dentin sialophosphoprotein precursor [Homo sapiens] Q9NZW4 0.0 83% ...

  20. Dissecting Choral Speech: Properties of the Accompanist Critical to Stuttering Reduction

    Science.gov (United States)

    Kiefte, Michael; Armson, Joy

    2008-01-01

    The effects of choral speech and altered auditory feedback (AAF) on stuttering frequency were compared to identify those properties of choral speech that make it a more effective condition for stuttering reduction. Seventeen adults who stutter (AWS) participated in an experiment consisting of special choral speech conditions that were manipulated…

  1. World War 2 in Alaska: A Historic and Resources Management Plan. Volume 1. A History of World War 2 in Alaska and Management Plan

    Science.gov (United States)

    1987-05-01

    Hitchcock , Inc. Golodoff, Innokenty 1966 The Last Days of Attu Village. Alaska Sportsman 32:8-9. *Goldberg, Alfred 1955 "AAF Aircraft of World War II...Navy, under the influence of nineteenth century theoretician Admiral Alfred Thayer Mahan, was dedicated to large battleships, to the exclusion of other

  2. Investigation of the interfacial reaction between metal and fluorine-contained polyimides

    Science.gov (United States)

    Yang, Ching-Yu; Chen, J. S.; Hsu, S. L. C.

    2005-07-01

    In this work, thin metal films (Cr and Ta) were deposited on fluorine-contained polyimides, 6FDA-BisAAF, and 6FDA-PPD. The chemical states of the metal/polyimide samples were characterized by using x-ray photoelectron spectroscopy (XPS). XPS analysis reveals that metal-C, C-O, and metal-O bondings are present in metallized 6FDA-BisAAF and 6FDA-PPD. C-F bonds are observed in bare 6FDA-BisAAF and 6FDA-PPD however, they are not seen in the metallized samples. Disappearance of the C-F bonding is attributed to the disruption of CF3 side groups from the main chains of 6FDA-BisAAF and 6FDA-PPD when the chains are exposed to the plasma during the metal deposition. Nevertheless, the disruption of CF3 side groups also creates sites for the formation of metal-C or C-O bondings, which provide a positive adhesion strength at the metal/polyimide interface, as revealed by the tape test.

  3. NCBI nr-aa BLAST: CBRC-GGOR-01-0320 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGOR-01-0320 ref|NP_001003054.1| prostaglandin E2 receptor EP4 subtype [Canis lupus... familiaris] gb|AAF00470.1|AF177934_1 prostaglandin E2 receptor EP4 subtype [Canis lupus familiaris] NP_001003054.1 3e-91 87% ...

  4. NCBI nr-aa BLAST: CBRC-MEUG-01-1975 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-1975 ref|NP_001003054.1| prostaglandin E2 receptor EP4 subtype [Canis lupus... familiaris] gb|AAF00470.1|AF177934_1 prostaglandin E2 receptor EP4 subtype [Canis lupus familiaris] NP_001003054.1 0.0 78% ...

  5. NCBI nr-aa BLAST: CBRC-PCAP-01-0700 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PCAP-01-0700 ref|NP_001003054.1| prostaglandin E2 receptor EP4 subtype [Canis lupus... familiaris] gb|AAF00470.1|AF177934_1 prostaglandin E2 receptor EP4 subtype [Canis lupus familiaris] NP_001003054.1 8e-84 79% ...

  6. NCBI nr-aa BLAST: CBRC-TTRU-01-1259 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1259 ref|NP_059467.1| NADH dehydrogenase subunit 6 [Paragonimus westerman...i] gb|AAF73395.1|AF219379_9 NADH dehydrogenase subunit 6 [Paragonimus westermani] NP_059467.1 0.076 27% ...

  7. NCBI nr-aa BLAST: CBRC-DNOV-01-0366 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0366 ref|NP_059467.1| NADH dehydrogenase subunit 6 [Paragonimus westerman...i] gb|AAF73395.1|AF219379_9 NADH dehydrogenase subunit 6 [Paragonimus westermani] NP_059467.1 0.75 24% ...

  8. NCBI nr-aa BLAST: CBRC-MDOM-04-0426 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MDOM-04-0426 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 1e-154 49% ...

  9. NCBI nr-aa BLAST: CBRC-PVAM-01-0255 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PVAM-01-0255 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 2e-79 33% ...

  10. NCBI nr-aa BLAST: CBRC-MEUG-01-0354 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-0354 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 1e-140 34% ...

  11. NCBI nr-aa BLAST: CBRC-MMUR-01-1375 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUR-01-1375 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 3e-84 34% ...

  12. NCBI nr-aa BLAST: CBRC-MEUG-01-1857 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-1857 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 4e-12 31% ...

  13. NCBI nr-aa BLAST: CBRC-PCAP-01-1243 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PCAP-01-1243 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 3e-40 22% ...

  14. NCBI nr-aa BLAST: CBRC-TTRU-01-0247 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0247 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 4e-38 33% ...

  15. NCBI nr-aa BLAST: CBRC-OPRI-01-0084 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0084 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 3e-69 27% ...

  16. NCBI nr-aa BLAST: CBRC-MEUG-01-0364 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-0364 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 5e-37 31% ...

  17. NCBI nr-aa BLAST: CBRC-MEUG-01-0827 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-0827 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 1e-138 42% ...

  18. NCBI nr-aa BLAST: CBRC-GGOR-01-0777 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGOR-01-0777 ref|NP_523475.3| salivary gland secretion 1 [Drosophila melanogaster] gb|AAF50957.4| saliv...ary gland secretion 1 [Drosophila melanogaster] NP_523475.3 1e-38 25% ...

  19. Cognitive Radio for Emergency Networks

    NARCIS (Netherlands)

    Zhang, Qiwei; Kokkeler, A.B.J.; Smit, G.J.M.

    2006-01-01

    In the scope of the Adaptive Ad-hoc Freeband (AAF) project, an emergency network built on top of Cognitive Radio is proposed to alleviate the spectrum shortage problem which is the major limitation for emergency networks. Cognitive Radio has been proposed as a promising technology to solve todayâ?~B

  20. A Proof-of-concept demonstrator for Cognitive Radio

    NARCIS (Netherlands)

    Heskamp, Marnix; Hoeksema, Fokke; Schiphorst, Roel; Slump, Kees

    2005-01-01

    The Adaptive Ad-hoc Free Band Wireless Communications (AAF) project is researching the possibility of using Software Defined Radio for wireless networks that needs to operate in an unknown or even hostile radio environment. Each node in the network needs to be cognitive of nearby transceivers in ord

  1. Kitchen Appliance Upgrades Improve Water Efficiency at DOD Exchange Facilities: Best Management Practice Case Study #11: Commercial Kitchen Equipment (Brochure)

    Energy Technology Data Exchange (ETDEWEB)

    2011-09-01

    The Exchange, formerly the Army and Air Force Exchange Service (AAFES), is a joint military activity and the U.S. Department of Defense?s (DOD) oldest and largest retailer. The Exchange is taking a leadership role in water efficiency improvements in their commercial kitchens by integrating water efficiency concepts into the organization?s overall sustainability plan and objectives.

  2. Relative cost-effectiveness of using an extensively hydrolyzed casein formula in managing infants with cow’s milk allergy in Brazil

    Science.gov (United States)

    Guest, Julian F; Yang, Ariana C; Oba, Jane; Rodrigues, Maraci; Caetano, Rosane; Polster, Lilian

    2016-01-01

    Objective To estimate the cost-effectiveness of three alternative dietetic strategies for cow’s milk allergy in Brazil: 1) using an extensively hydrolyzed casein formula (eHCF; Nutramigen) as a first-line formula, but switching to an amino acid formula (AAF) if infants remain symptomatic; 2) using an AAF as a first-line formula and then switching to an eHCF after 4 weeks once infants are symptom-free, but switching back to an AAF if infants become symptomatic; and 3) using an AAF as a first-line formula and keeping all infants on that formula. The analysis was conducted from the perspective of the Brazilian public health care system, Sistema Único de Saude. Methods Decision modeling was used to estimate the probability of immunoglobulin E (IgE)-mediated and non-IgE-mediated allergic infants developing tolerance to cow’s milk by 12 months from starting a formula. The models also estimated the Sistema Único de Saude cost (at 2013/2014 prices) of managing infants over 12 months after starting a formula, as well as the relative cost-effectiveness of each of the dietetic strategies. Results The probability of developing tolerance to cow’s milk by 12 months from starting a formula was higher among infants with either IgE-mediated or non-IgE-mediated allergy who were initially fed with an eHCF, compared with those who were initially fed with an AAF. The total health care cost of initially feeding an eHCF to cow’s milk allergic infants was less than that of initially feeding both IgE-mediated and non-IgE-mediated infants with an AAF. Conclusion Within the study’s limitations, using an eHCF instead of an AAF for the first-line management of newly-diagnosed infants with cow’s milk allergy affords a cost-effective use of publicly funded resources, since it improves the outcome for less cost. PMID:27799804

  3. Anti-inflammatory effect of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl sitosterol] from Agave angustifolia on ear edema in mice.

    Science.gov (United States)

    Hernández-Valle, Elizabeth; Herrera-Ruiz, Maribel; Salgado, Gabriela Rosas; Zamilpa, Alejandro; Ocampo, Martha Lucia Arenas; Aparicio, Antonio Jiménez; Tortoriello, Jaime; Jiménez-Ferrer, Enrique

    2014-09-29

    In Mexico Agave angustifolia has traditionally been used to treat inflammation. The aim of this study was to measure the anti-inflammatory effect of the extract of A. angustifolia, the isolation and identification of active compounds. From the acetone extract two active fractions were obtained, (AsF13 and AaF16). For the characterization of pharmacological activity, the acute inflammatory model of mouse ear edema induced with TPA was used. The tissue exposed to TPA and treatments were subjected to two analysis, cytokine quantification (IL-1β, IL-6, IL-10 and TNF-α) and histopathological evaluation. The active fraction (AaF16) consisted principally of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranpsyl] sitosterol. In AaF13 fraction was identified β-sitosteryl glucoside (2) and stigmasterol (3). The three treatments tested showed a concentration-dependent anti-inflammatory effect (AaAc Emax = 33.10%, EC50 = 0.126 mg/ear; AaF13 Emax = 54.22%, EC50 = 0.0524 mg/ear; AaF16 Emax = 61.01%, EC50 = 0.050 mg/ear). The application of TPA caused a significant increase on level of IL-1β, IL-6 and TNFα compared with basal condition, which was countered by any of the experimental treatments. Moreover, the experimental treatments induced a significant increase in the levels of IL-4 and IL-10, compared to the level observed when stimulated with TPA. Therefore, the anti-inflammatory effect of Agave angustifolia, is associated with the presence of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl] sitosterol.

  4. Anti-Inflammatory Effect of 3-O-[(6'-O-Palmitoyl-β-D-glucopyranosyl Sitosterol] from Agave angustifolia on Ear Edema in Mice

    Directory of Open Access Journals (Sweden)

    Elizabeth Hernández-Valle

    2014-09-01

    Full Text Available In Mexico Agave angustifolia has traditionally been used to treat inflammation. The aim of this study was to measure the anti-inflammatory effect of the extract of A. angustifolia, the isolation and identification of active compounds. From the acetone extract two active fractions were obtained, (AsF13 and AaF16. For the characterization of pharmacological activity, the acute inflammatory model of mouse ear edema induced with TPA was used. The tissue exposed to TPA and treatments were subjected to two analysis, cytokine quantification (IL-1β, IL-6, IL-10 and TNF-α and histopathological evaluation. The active fraction (AaF16 consisted principally of 3-O-[(6'-O-palmitoyl-β-D-glucopyranpsyl] sitosterol. In AaF13 fraction was identified β-sitosteryl glucoside (2 and stigmasterol (3. The three treatments tested showed a concentration-dependent anti-inflammatory effect (AaAc Emax = 33.10%, EC50 = 0.126 mg/ear; AaF13 Emax = 54.22%, EC50 = 0.0524 mg/ear; AaF16 Emax = 61.01%, EC50 = 0.050 mg/ear. The application of TPA caused a significant increase on level of IL-1β, IL-6 and TNFα compared with basal condition, which was countered by any of the experimental treatments. Moreover, the experimental treatments induced a significant increase in the levels of IL-4 and IL-10, compared to the level observed when stimulated with TPA. Therefore, the anti-inflammatory effect of Agave angustifolia, is associated with the presence of 3-O-[(6'-O-palmitoyl-β-D-glucopyranosyl] sitosterol.

  5. Prevalence of enteroaggregative Escherichia coli and its virulence-related genes in a case-control study among children from north-eastern Brazil.

    Science.gov (United States)

    Lima, Ila Fernanda Nunes; Boisen, Nadia; Quetz, Josiane da Silva; Havt, Alexandre; de Carvalho, Eunice Bobo; Soares, Alberto Melo; Lima, Noélia Leal; Mota, Rosa Maria Salani; Nataro, James P; Guerrant, Richard Littleton; Lima, Aldo Ângelo Moreira

    2013-05-01

    Enteroaggregative Escherichia coli (EAEC) is an important agent that causes endemic and epidemic diarrhoeal diseases worldwide. Several EAEC virulence-related genes (VRGs) have been described but their role in the clinical outcome of infection is not completely defined. This study investigated the prevalence of EAEC and potential associations of its VRGs with risk of or protection from diarrhoeal diseases in children from urban communities in north-eastern Brazil. The case-control study included 166 children, who had their stools evaluated for the EAEC diagnostic genes (aaiC and aatA) using PCR. Positive samples were further analysed by multiplex PCR and identified 18 VRGs. EAEC was found in the same proportion in both groups (41%). The plasmid-borne gene encoding a hexosyltransferase homologue (capU) was the most frequently detected (89.6%), followed by dispersin protein (aap, 58.2%) and EAEC HilA homologue (eilA, 57.8%). The AAF/III fimbrial subunit (agg3A) gene was observed at lower frequency (1.5%). Plasmid-encoded toxin (pet) or AAF/II fimbrial subunit (aafA) was associated significantly with disease. AAF/IV fimbrial subunit (agg4A) or hypothetical plasmid-encoded haemolysin (orf61) was detected significantly more in controls than in children with diarrhoea. In addition, one set of genes in combination, aaiC and agg3/4C but lacking agg4A and orf61, was associated with diarrhoea cases; and another one, orf61 in the absence of pet and aafA, was correlated with control children. These data confirm a high prevalence, endemicity and heterogeneity of EAEC strains in the developing urban areas of north-eastern Brazil. Statistical correlation between cases and controls was seen with either isolated or combined sets of genes, suggesting that the pathophysiology of EAEC infection involves a complex and dynamic modulation of several VRGs.

  6. Prevalence of enteroaggregative Escherichia coli and its virulence-related genes in a case–control study among children from north-eastern Brazil

    Science.gov (United States)

    Boisen, Nadia; Quetz, Josiane da Silva; Havt, Alexandre; de Carvalho, Eunice Bobo; Soares, Alberto Melo; Mota, Rosa Maria Salani; Nataro, James P.; Guerrant, Richard Littleton

    2013-01-01

    Enteroaggregative Escherichia coli (EAEC) is an important agent that causes endemic and epidemic diarrhoeal diseases worldwide. Several EAEC virulence-related genes (VRGs) have been described but their role in the clinical outcome of infection is not completely defined. This study investigated the prevalence of EAEC and potential associations of its VRGs with risk of or protection from diarrhoeal diseases in children from urban communities in north-eastern Brazil. The case–control study included 166 children, who had their stools evaluated for the EAEC diagnostic genes (aaiC and aatA) using PCR. Positive samples were further analysed by multiplex PCR and identified 18 VRGs. EAEC was found in the same proportion in both groups (41 %). The plasmid-borne gene encoding a hexosyltransferase homologue (capU) was the most frequently detected (89.6 %), followed by dispersin protein (aap, 58.2 %) and EAEC HilA homologue (eilA, 57.8 %). The AAF/III fimbrial subunit (agg3A) gene was observed at lower frequency (1.5 %). Plasmid-encoded toxin (pet) or AAF/II fimbrial subunit (aafA) was associated significantly with disease. AAF/IV fimbrial subunit (agg4A) or hypothetical plasmid-encoded haemolysin (orf61) was detected significantly more in controls than in children with diarrhoea. In addition, one set of genes in combination, aaiC and agg3/4C but lacking agg4A and orf61, was associated with diarrhoea cases; and another one, orf61 in the absence of pet and aafA, was correlated with control children. These data confirm a high prevalence, endemicity and heterogeneity of EAEC strains in the developing urban areas of north-eastern Brazil. Statistical correlation between cases and controls was seen with either isolated or combined sets of genes, suggesting that the pathophysiology of EAEC infection involves a complex and dynamic modulation of several VRGs. PMID:23429698

  7. Regulatory effect of connexin/gap junction intercellular communication on proliferation of rat hepatic oval cells%连接蛋白/缝隙连接细胞间通讯对大鼠肝卵圆细胞增殖调控作用

    Institute of Scientific and Technical Information of China (English)

    傅华群; 李学东; 王开阳; 蒋星星; 巢映辉

    2011-01-01

    BACKGROUND: Gap junction intercellular communication (GJIC) mediated by connexin (CX) is the pivotal pattern ofcommunication in multicellular organism.OBJECTIVE: To observe the regulatory effect of CX/GJIC on hepatic oval cell proliferation.METHODS: Wistar rats were assigned to four groups. Control group received normal diet. Animal models of hepatic oval cell proliferation were established in the 2-acetylaminofluorene/ partial hepatectomy (2 -AAF/PH) group according to modified Solt-Farber method. Phenobarbital group received phenobarbital drinking for 7 days, and on day 8, models were established as the 2 -AAF/PH group. Phenobarbital drinking lasted till the end of the test. Panax notoginseng saponins (PNS) group received PNS 25 mg/kg daily by intraperitoneal injection during model establishment as the 2-AAF/PH group, lasting till the end of the test.RESULTS AND CONCLUSION: The patterns of CX expression were different in spatial and temporal in the rat liver after 2-AAF/PH, decreased and then recovered. The CX43 protein was expressed in hepatic oval cells, and they were increased at first and then to recover. GJIC was inhibited by altered the spatial and temporal expression patterns of CX in the rats liver after 2-AAF/PH (with phenobarbital) that can accelerate the proliferation of hepatic oval cells. GJIC was increased by enhancing the expression level of CX in the rats liver after2 -AAF/PH (with PNS) that can accurately activate hepatic oval cells proliferation. The peak of hepatic oval cells pro liferation was decreased, delayed and lasted long time. To decrease the GJIC in the rat liver of 2-AAF/PH that can accelerate and enhance the proliferation of hepatic oval cells, whereas to increase the GJIC can decrease,delay and last long time the proliferation of hepatic oval cells. These suggest that the proliferation and differentiation of hepatic oval cells were modulated by CX/GJIC.%背景:缝隙连接蛋白介导的缝隙连接细胞间通讯(gap junction

  8. Growth-related alterations during liver carcinogenesis: Effect of promoters

    Energy Technology Data Exchange (ETDEWEB)

    Seglen, P.O.; Gerlyng, P. (Norwegian Radium Hospital, Oslo (Norway))

    1990-08-01

    Bromodeoxyuridine labeling of DNA, binuclearity counting, and flow cytometric analysis of isolated hepatocytes and hepatocyte nuclei has been used to assess heptocellular growth patterns related to liver carcinogenesis. Three growth patterns can be distinguished. Mononucleating growth is observed during liver regeneration and after treatment with the tumor promoter 2-acetylaminofluorene (2-AAF) and its analogue 4-AAF. In this growth mode binucleation does not occur, resulting in a decrease in the fraction of binucleated cells. Binucleating growth is observed during normal liver development and after treatment with compounds such as phenobarbital, characterized by progressive polyploidization and maintenance of a binucleated cell fraction. Diploid growth is the growth pattern of neoplastic liver hepatocytes. Most of the cells in neoplastic lesions (foci, nodules, and carcinomas) are diploid, in contrast to the normal liver. Diploid tumor cells have a much higher proliferative activity than tetraploid tumor cells, suggesting that the latter may posses a limited growth potential that makes abrogation of binucleation proliferatively advantageous.

  9. Topography of acoustic response characteristics in the auditory cortex of the Kunming mouse

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Topography of acoustic response characteristics in the auditory cortex (AC) of the Kunming (KM) mouse has been examined by using microelectrode recording techniques.Based on best-frequency (BF) maps,both the primary auditory field (AⅠ) and the anterior auditory field (AAF) are tonotopically organized with a counter running frequency gradient.Within an isofrequency stripe,the width of the frequency-threshold curves of single neurons increases,and minimum threshold (MT) decreases towards more ventral locations.BFs in AⅠand AAF range from 4 to 38 kHz.Auditory neurons with BFs above 40 kHz are located at the rostrodorsal part of the AC.The findings suggest that the KM mouse is a good model suitable for auditory research.

  10. Repair of DNA lesions induced by ultraviolet irradiation and aromatic amines in normal and repair-deficient human lymphoblastoid cell lines

    DEFF Research Database (Denmark)

    Stevnsner, Tinna; Frandsen, Henrik; Autrup, Herman

    1995-01-01

    A host cell reactivation (HCR) assay was employed to study the capacity of a normal and three repair-deficient human lymphoblastoid cell lines to repair DNA damage induced by UV irradiation and the aromatic amines 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and N-acetyl-2-aminofluorene....... In the XP-D cell line, which had practically no DNA repair capacity, AAF adducts had a more potent inhibitory effect on gene expression than UV and PhIP adducts. When corrected for this inhibitory effect, the wild-type, XP-C and CS-B cell lines repaired low levels of AAF and UV adducts with similar...

  11. Gene flow, subspecies composition, and dengue virus-2 susceptibility among Aedes aegypti collections in Senegal.

    Directory of Open Access Journals (Sweden)

    Massamba Sylla

    Full Text Available BACKGROUND: Aedes aegypti, the "yellow fever mosquito", is the primary vector to humans of the four serotypes of dengue viruses (DENV1-4 and yellow fever virus (YFV and is a known vector of Chikungunya virus. There are two recognized subspecies of Ae. aegypti sensu latu (s.l.: the presumed ancestral form, Ae. aegypti formosus (Aaf, a primarily sylvan mosquito in sub-Saharan Africa, and Ae. aegypti aegypti (Aaa, found globally in tropical and subtropical regions typically in association with humans. The designation of Ae. aegypti s.l. subspecies arose from observations made in East Africa in the late 1950s that the frequency of pale "forms" of Ae. aegypti was higher in populations in and around human dwellings than in those of the nearby bush. But few studies have been made of Ae. aegypti s.l. in West Africa. To address this deficiency we have been studying the population genetics, subspecies composition and vector competence for DENV-2 of Ae. aegypti s.l. in Senegal. METHODS AND FINDINGS: A population genetic analysis of gene flow was conducted among 1,040 Aedes aegypti s.l. from 19 collections distributed across the five phytogeographic regions of Senegal. Adults lacking pale scales on their first abdominal tergite were classified as Aedes aegypti formosus (Aaf following the original description of the subspecies and the remainder were classified as Aedes aegypti aegypti (Aaa. There was a clear northwest-southeast cline in the abundance of Aaa and Aaf. Collections from the northern Sahelian region contained only Aaa while southern Forest gallery collections contained only Aaf. The two subspecies occurred in sympatry in four collections north of the Gambia in the central Savannah region and Aaa was a minor component of two collections from the Forest gallery area. Mosquitoes from 11 collections were orally challenged with DENV-2 virus. In agreement with the early literature, Aaf had significantly lower vector competence than Aaa. Among pure Aaa

  12. Aggregate Resources Study, Cave and Steptoe Valleys, Nevada.

    Science.gov (United States)

    1981-09-25

    Quartzite Creek Range 8 CV-AS Bgan Range Do Dolomite 9 CV-A9 Cave Valley Ls Limestone 10 Cv-Xf0- Egan Range Qtz Quartzite 11 CV-All Egan Range LS...Limestone 12 CV-A12 Cave Valley Aaf a Sandy Gravel G-GM 13 CV-A13 Bgan Range Vii Quartz Latite 14 CV-A14 Cave Valley Ls Limestone 7r 7 FIELD OBSERVATIONS...SO-A2 Bgan Range Vu Dacitic Ash-flow Tuff 25 SO-A3 Steptoe Aalf Sandy Gravel GP-GM Valley 26 SO-A4 Steptoe Aafs Sandy Gravel GE-GM Valley 27 S0-A5

  13. Main: 1OGP [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1OGP シロイヌナズナ Arabidopsis Arabidopsis thaliana (L.) Heynh. Sulfite Oxidase Name=F1c9...BL; AB071965; BAC10904.1; -.|EMBL; AC010797; AAF03458.1; -.|EMBL; AY133863; AAM91797.1; -.|EMBL; AF360247; AAK25957.1; -.|PDB; 1OGP..., R-Free: 0.26617 Length: 393 AA, Molecular weight: 43329 Da MPGIRGPSEYSQEPPRHPSL...KVNAKEPFNAEPPRSALVSSYVTPVDLFYKRNHGPIPIVDHLQSYSVTLTGLIQNPRKLFIKDIRSLPKYNVTATLQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWG...GAKLADVLELVGIPKLTASTNLGARHVEFVSVDRCKEENGGPYKASITLSQATNPEADVLLAYEMNGETLNRDHGFPLRVVVPGVIGARSVKWLDSINVIAEESQGFF

  14. EST Table: BY929840 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BY929840 E_EL_ovS0_01F10_R_0 10/09/28 89 %/127 aa ref|NP_477114.1| noisette [Drosop...hila melanogaster] sp|O46106.1|NOI_DROME RecName: Full=Splicing factor 3A subunit 3; AltName: Full=Protein noise...tte emb|CAA11045.1| noisette [Drosophila melanogaster] gb|AAF51999.1| noisette [Drosophila melanogaster

  15. EST Table: BY929895 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BY929895 E_EL_ovS0_02E03_R_0 10/09/28 70 %/178 aa ref|NP_477114.1| noisette [Drosop...hila melanogaster] sp|O46106.1|NOI_DROME RecName: Full=Splicing factor 3A subunit 3; AltName: Full=Protein noise...tte emb|CAA11045.1| noisette [Drosophila melanogaster] gb|AAF51999.1| noisette [Drosophila melanogaster

  16. EST Table: CK499375 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK499375 rswbb0_009754.y1 11/12/09 n.h 10/09/29 88 %/124 aa ref|NP_477114.1| noiset...te [Drosophila melanogaster] sp|O46106.1|NOI_DROME RecName: Full=Splicing factor 3A subunit 3; AltName: Full=Protein noise...tte emb|CAA11045.1| noisette [Drosophila melanogaster] gb|AAF51999.1| noisette [Drosophila mel

  17. NCBI nr-aa BLAST: CBRC-DYAK-02-0062 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-02-0062 ref|NP_725630.1| vegetable CG6657-PA, isoform A [Drosophila melan...ogaster] ref|NP_524685.2| vegetable CG6657-PB, isoform B [Drosophila melanogaster] sp|Q9V7W1|PIGV_DROME GPI ...mannosyltransferase 2 (GPI mannosyltransferase II) (GPI-MT-II) (Protein vegetable) gb|AAF57928.1| CG6657-PA,

  18. NCBI nr-aa BLAST: CBRC-AGAM-02-0019 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-02-0019 ref|NP_725630.1| vegetable CG6657-PA, isoform A [Drosophila melan...ogaster] ref|NP_524685.2| vegetable CG6657-PB, isoform B [Drosophila melanogaster] sp|Q9V7W1|PIGV_DROME GPI ...mannosyltransferase 2 (GPI mannosyltransferase II) (GPI-MT-II) (Protein vegetable) gb|AAF57928.1| CG6657-PA,

  19. EUCOM Keystone: Connecting Across Services Enabling Timely Horizontal & Vertical Integration, Product Reference Guide, Revision 1

    Science.gov (United States)

    2015-09-01

    geospatial data anywhere in the world. Keystone implants the Google Earth KML (Keyhole Markup Language) publishing interface to consume the Keystone Work...Product sharing. Client type Google Earth KML interface Data Format Consume Keystone Work Product XML data format Communication Flow...School Liaison o Clinic o AAFES (facilities include: PX, clothing store, laundromat, car care center, and school cafeteria) o Child and Youth

  20. NCBI nr-aa BLAST: CBRC-AGAM-03-0016 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-03-0016 ref|NP_477376.1| CG4585-PA [Drosophila melanogaster] dbj|BAA32689....1| unnamed protein product [Drosophila melanogaster] dbj|BAA32692.1| unnamed protein product [Drosophila me...lanogaster] gb|AAF47081.1| CG4585-PA [Drosophila melanogaster] gb|AAL28300.1| GH20310p [Drosophila melanogaster] NP_477376.1 1e-132 58% ...

  1. NCBI nr-aa BLAST: CBRC-DMEL-02-0084 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-02-0084 ref|NP_477376.1| CG4585-PA [Drosophila melanogaster] dbj|BAA32689....1| unnamed protein product [Drosophila melanogaster] dbj|BAA32692.1| unnamed protein product [Drosophila me...lanogaster] gb|AAF47081.1| CG4585-PA [Drosophila melanogaster] gb|AAL28300.1| GH20310p [Drosophila melanogaster] NP_477376.1 0.0 100% ...

  2. Altered Sensory Feedbacks in Pianist's Dystonia: the altered auditory feedback paradigm and the glove effect

    Directory of Open Access Journals (Sweden)

    Felicia Pei-Hsin Cheng

    2013-12-01

    Full Text Available Background: This study investigates the effect of altered auditory feedback (AAF in musician's dystonia (MD and discusses whether altered auditory feedback can be considered as a sensory trick in MD. Furthermore, the effect of AAF is compared with altered tactile feedback, which can serve as a sensory trick in several other forms of focal dystonia. Methods: The method is based on scale analysis (Jabusch et al. 2004. Experiment 1 employs synchronization paradigm: 12 MD patients and 25 healthy pianists had to repeatedly play C-major scales in synchrony with a metronome on a MIDI-piano with 3 auditory feedback conditions: 1. normal feedback; 2. no feedback; 3. constant delayed feedback. Experiment 2 employs synchronization-continuation paradigm: 12 MD patients and 12 healthy pianists had to repeatedly play C-major scales in two phases: first in synchrony with a metronome, secondly continue the established tempo without the metronome. There are 4 experimental conditions, among them 3 are the same altered auditory feedback as in Experiment 1 and 1 is related to altered tactile sensory input. The coefficient of variation of inter-onset intervals of the key depressions was calculated to evaluate fine motor control. Results: In both experiments, the healthy controls and the patients behaved very similarly. There is no difference in the regularity of playing between the two groups under any condition, and neither did AAF nor did altered tactile feedback have a beneficial effect on patients’ fine motor control. Conclusions: The results of the two experiments suggest that in the context of our experimental designs, AAF and altered tactile feedback play a minor role in motor coordination in patients with musicians' dystonia. We propose that altered auditory and tactile feedback do not serve as effective sensory tricks and may not temporarily reduce the symptoms of patients suffering from MD in this experimental context.

  3. NCBI nr-aa BLAST: CBRC-ATHA-01-0017 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ATHA-01-0017 ref|NP_564207.1| unknown protein [Arabidopsis thaliana] gb|AAF871...60.1|AC002423_25 T23E23.1 [Arabidopsis thaliana] dbj|BAD42966.1| unknown protein [Arabidopsis thaliana] dbj|...BAD43185.1| unknown protein [Arabidopsis thaliana] dbj|BAD44677.1| unknown protein [Arabidopsis thaliana] NP_564207.1 0.0 100% ...

  4. NCBI nr-aa BLAST: CBRC-AGAM-01-0022 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-01-0022 ref|NP_523974.3| fear-of-intimacy CG6817-PA [Drosophila melanogas...ter] sp|Q9VSL7|FOI_DROME Zinc transporter foi precursor (Protein fear-of-intimacy) (Protein kastchen) gb|AAF50401.3| CG6817-PA [Drosophila melanogaster] NP_523974.3 1e-111 44% ...

  5. Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections

    Science.gov (United States)

    Vidal, Maricel; Kruger, Eileen; Durán, Claudia; Lagos, Rosanna; Levine, Myron; Prado, Valeria; Toro, Cecilia; Vidal, Roberto

    2005-01-01

    We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx1, stx2, and eae), enteropathogenic (eae and bfp), enterotoxigenic (stII and lt), enteroinvasive (virF and ipaH), enteroaggregative (aafII), and diffuse adherent (daaE) Escherichia coli in stool samples. PMID:16208019

  6. Quantitative map of multiple auditory cortical regions with a stereotaxic fine-scale atlas of the mouse brain

    OpenAIRE

    Hiroaki Tsukano; Masao Horie; Ryuichi Hishida; Kuniyuki Takahashi; Hirohide Takebayashi; Katsuei Shibuki

    2016-01-01

    Optical imaging studies have recently revealed the presence of multiple auditory cortical regions in the mouse brain. We have previously demonstrated, using flavoprotein fluorescence imaging, at least six regions in the mouse auditory cortex, including the anterior auditory field (AAF), primary auditory cortex (AI), the secondary auditory field (AII), dorsoanterior field (DA), dorsomedial field (DM), and dorsoposterior field (DP). While multiple regions in the visual cortex and somatosensory ...

  7. An Optimization of the Maintenance Assets Distribution Network in the Argentine Air Force

    Science.gov (United States)

    2015-03-26

    contractor who performs the required maintenance. Additionally, each weapon 7 system has assigned depot warehouses for parts. Both the depot shops...stock, the local warehouse places an order to the assigned Logistic Unit through the SIL, which is the information technology system established in... management and decentralized execution, continuous improvement and maximum system efficiency to achieve the goal with the least investment of resources (AAF

  8. Bronze Civilization Shines Asia Art Festival

    Institute of Scientific and Technical Information of China (English)

    Hu Chunliang

    2009-01-01

    @@ The 11th Asia Arts Festival (AAF) was held from August 18 through 26, 2009 in Ordos, a city in the northern China's Inner Mongolia autonomous region. This event was jointly sponsored by the Ministry of Culture of the People's Republic of China and the People's Government of Inner Mongolia Autonomous Region, and organized by the Ordos Municipal People's Government, Culture Department of Inner Mongolia Autonomous Region and China Arts and Entertainment Group.

  9. Evaluation of Support Provided to Mobilized Army National Guard and U.S. Army Reserve Units

    Science.gov (United States)

    2005-08-05

    with family members. CENTCOM and the Space and Naval Warfare Systems Command have significantly improved access to communications with family as the...contract with Bahrain Maritime and Mercantile International, beginning in August 2002 through December 2003.10 Seven Seas Shiphandlers became the...Switching Network (DSN) • MWR phone banks through the Army and Air Force Exchange System (AAFES) • Internet cafes (provided by the Navy Space and Naval

  10. Use of ARM Mobile Facility (AMF) Data to Study Aerosol Indirect Effects in China

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zhanqing [Univ. of Maryland, College Park, MD (United States)

    2012-12-19

    General goals: 1) Facilitating the deployment of the ARM Mobile Facility (AMF) and Ancillary Facility (AAF) in China in 2008, 2) Processing, retrieving, improving and analyzing observation data from ground-based, air-borne and space-borne instruments; 3) Conducting a series of studies to gain insights into the direct and indirect effects of these aerosols on radiation, clouds, and precipitation using both

  11. The commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coli

    Directory of Open Access Journals (Sweden)

    Fletcher Jonathan N

    2009-12-01

    Full Text Available Abstract Background The roles of diffusely-adherent Escherichia coli (DAEC and enteroaggregative E. coli (EAEC in disease are not well understood, in part because of the limitations of diagnostic tests for each of these categories of diarrhoea-causing E. coli. A HEp-2 adherence assay is the Gold Standard for detecting both EAEC and DAEC but DNA probes with limited sensitivity are also employed. Results We demonstrate that the daaC probe, conventionally used to detect DAEC, cross-reacts with a subset of strains belonging to the EAEC category. The cross hybridization is due to 84% identity, at the nucleotide level, between the daaC locus and the aggregative adherence fimbriae II cluster gene, aafC, present in some EAEC strains. Because aaf-positive EAEC show a better association with diarrhoea than other EAEC, this specific cross-hybridization may have contributed to an over-estimation of the association of daaC with disease in some studies. We have developed a discriminatory PCR-RFLP protocol to delineate EAEC strains detected by the daaC probe in molecular epidemiological studies. Conclusions A PCR-RFLP protocol described herein can be used to identify aaf-positive EAEC and daaC-positive DAEC and to delineate these two types of diarrhoeagenic E. coli, which both react with the daaC probe. This should help to improve current understanding and future investigations of DAEC and EAEC epidemiology.

  12. Use of iron and bio-oil wastes to produce highly dispersed Fe/C composites for the photo-Fenton reaction.

    Science.gov (United States)

    de Mendonça, Fernanda Gomes; Rosmaninho, Marcelo Gonçalves; da Fonseca, Philipe Xavier; Soares, Ricardo Reis; Ardisson, José Domingos; Tristão, Juliana Cristina; Lago, Rochel Montero

    2016-04-07

    This work describes the synthesis, characterization, and application of an active heterogeneous photo-Fenton system obtained from two different wastes, i.e., laterite (an iron mining waste) and the acid aqueous fraction (AAF) from bio-oil production. AAF with high acidity (ca. 3 molH+ L(-1)) and organic concentration (25 wt.%) obtained from biomass flash pyrolysis was used for the efficient extraction of Fe(3+) from laterite waste. After extraction, the mixture Fe(3+)/AAF was dried and treated at different temperatures, i.e., 500, 650, and 800 °C, to obtain Fe/C reactive composites. Mössbauer, XRD, TG, elemental analyses, and SEM/EDS showed the presence of highly disperse Fe oxide nanoparticles at 500 and 650 °C and Fe(0) particles in the material obtained at 800 °C with carbon contents varying from 74 to 80 %. The three composites were tested as heterogeneous catalysts in the photo-Fenton reaction for the oxidation of the model dye contaminant methylene blue, showing high activities at neutral pH.

  13. Effects of altered auditory feedback across effector systems: production of melodies by keyboard and singing.

    Science.gov (United States)

    Pfordresher, Peter Q; Mantell, James T

    2012-01-01

    We report an experiment that tested whether effects of altered auditory feedback (AAF) during piano performance differ from its effects during singing. These effector systems differ with respect to the mapping between motor gestures and pitch content of auditory feedback. Whereas this action-effect mapping is highly reliable during phonation in any vocal motor task (singing or speaking), mapping between finger movements and pitch occurs only in limited situations, such as piano playing. Effects of AAF in both tasks replicated results previously found for keyboard performance (Pfordresher, 2003), in that asynchronous (delayed) feedback slowed timing whereas alterations to feedback pitch increased error rates, and the effect of asynchronous feedback was similar in magnitude across tasks. However, manipulations of feedback pitch had larger effects on singing than on keyboard production, suggesting effector-specific differences in sensitivity to action-effect mapping with respect to feedback content. These results support the view that disruption from AAF is based on abstract, effector independent, response-effect associations but that the strength of associations differs across effector systems.

  14. Exploring flavour-producing core microbiota in multispecies solid-state fermentation of traditional Chinese vinegar.

    Science.gov (United States)

    Wang, Zong-Min; Lu, Zhen-Ming; Shi, Jin-Song; Xu, Zheng-Hong

    2016-05-31

    Multispecies solid-state fermentation (MSSF), a natural fermentation process driven by reproducible microbiota, is an important technique to produce traditional fermented foods. Flavours, skeleton of fermented foods, was mostly produced by microbiota in food ecosystem. However, the association between microbiota and flavours and flavour-producing core microbiota are still poorly understood. Here, acetic acid fermentation (AAF) of Zhenjiang aromatic vinegar was taken as a typical case of MSSF. The structural and functional dynamics of microbiota during AAF process was determined by metagenomics and favour analyses. The dominant bacteria and fungi were identified as Acetobacter, Lactobacillus, Aspergillus, and Alternaria, respectively. Total 88 flavours including 2 sugars, 9 organic acids, 18 amino acids, and 59 volatile flavours were detected during AAF process. O2PLS-based correlation analysis between microbiota succession and flavours dynamics showed bacteria made more contribution to flavour formation than fungi. Seven genera including Acetobacter, Lactobacillus, Enhydrobacter, Lactococcus, Gluconacetobacer, Bacillus and Staphylococcus were determined as functional core microbiota for production of flavours in Zhenjiang aromatic vinegar, based on their dominance and functionality in microbial community. This study provides a perspective for bridging the gap between the phenotype and genotype of ecological system, and advances our understanding of MSSF mechanisms in Zhenjiang aromatic vinegar.

  15. The Herbal Compound “Diwu Yanggan” Modulates Liver Regeneration by Affecting the Hepatic Stem Cell Microenvironment in 2-Acetylaminofluorene/Partial Hepatectomy Rats

    Directory of Open Access Journals (Sweden)

    Bin-Bin Zhao

    2015-01-01

    Full Text Available Ethnopharmacological Relevance. “Diwu Yanggan” (DWYG has been reported to regulate liver regeneration, modulate the immune response, ameliorate liver injury, kill virus, ameliorate liver fibrosis, and suppress hepatic cancer. However, its mechanisms are still unknown. Objectives. To investigate the effects of DWYG on oval cell proliferation in 2-AAF/PH rats and determine its mechanism. Methods. Wistar rats were randomly distributed into normal group, sham group, vehicle group, and DWYG group. Hepatic pathological changes were examined by H&E staining. The oval cell markers CD34, AFP, CK-19 and hematopoietic cell markers CD45, Thy1.1, and hepatocyte marker ALB were examined with immunohistochemistry. The percentage of CD34/CD45 double-positive cells in bone marrow was detected by flow cytometry. Cytokine levels were measured with the Bio-plex suspension array system. Results. DWYG significantly increased the survival rates of 2-AAF/PH rats and promoted liver regeneration. Furthermore, DWYG increased the ratio of CD34/CD45 double-positive cells on days 10 and 14. In addition, DWYG gradually restored IL-1, GRO/KC, and VEGF levels to those of the normal group. Conclusions. DWYG increases 2-AAF/PH rat survival rates, suppresses hepatic precarcinoma changes, and restores hepatic tissue structure and function. DWYG may act by modulating the hepatic microenvironment to support liver regeneration.

  16. Serum Potassium Levels Inversely Correlate with D-Dimer In Patients with Acute-Onset Atrial Fibrillation

    Energy Technology Data Exchange (ETDEWEB)

    Cervellin, Gianfranco, E-mail: gcervellin@ao.pr.it; Bonfanti, Laura; Picanza, Alessandra; Lippi, Giuseppe [1Academic Hospital of Parma (Italy)

    2015-03-15

    D-dimer values are frequently increased in patients with atrial fibrillation (AF) compared to subjects in sinus rhythm. Hypokalemia plays a role in several cardiovascular diseases, but little is known about the association with AF. D-dimer values are frequently increased in patients with atrial fibrillation (AF) compared with subjects in sinus rhythm. Hypokalemia plays a role in several cardiovascular diseases, but little is known about the association with AF. The aim of this study was to investigate correlations between D-dimer and serum potassium in acute-onset AF (AAF). To investigate the potential correlation between the values of serum potassium and D-dimer in patients with AAF, we retrospectively reviewed clinical and laboratory data of all emergency department visits for AAF in 2013. Among 271 consecutive AAF patients with D-dimer assessments, those with hypokalemia (n = 98) had significantly higher D-dimer values than normokalemic patients (139 versus 114 ng/mL, p = 0.004). The rate of patients with D-dimer values exceeding the diagnostic cut-off was higher in the group of patients with hypokalemia than in those with normal serum potassium (26.5% versus 16.2%; p = 0.029). An inverse and highly significant correlation was found between serum potassium and D-dimer (r = −0.21; p < 0.001), even after adjustments for age and sex (beta coefficient −94.8; p = 0.001). The relative risk for a positive D-dimer value attributed to hypokalemia was 1.64 (95% CI, 1.02 to 2.63; p = 0.040). The correlation remained statistically significant in patients free from antihypertensive drugs (r = −0.25; p = 0.018), but not in those taking angiotensin-receptor blockers, angiotensin-converting enzyme inhibitors, or diuretics. The inverse correlation between values of potassium and D-dimer in patients with AAF provides important and complementary information about the thromboembolic risk of these patients.

  17. Relative cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG in managing infants with cow’s milk allergy in Poland

    Science.gov (United States)

    Guest, Julian F; Weidlich, Diana; Kaczmarski, Maciej; Jarocka-Cyrta, Elzbieta; Kobelska-Dubiel, Natalia; Krauze, Agnieszka; Sakowska-Maliszewska, Iwona; Zawadzka-Krajewska, Anna

    2016-01-01

    Objective To estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (eHCF) containing the probiotic Lactobacillus rhamnosus GG (eHCF + LGG; Nutramigen LGG) as an initial treatment for cow’s milk allergy compared with eHCF alone and amino acid formulas (AAF) in Poland from the perspective of the Polish National Health Fund (Narodowy Fundusz Zdrowia [NFZ]) and parents. Methods Decision modeling was used to estimate the probability of cow’s milk allergic infants developing tolerance to cow’s milk by 18 months. The model also estimated the cost to the NFZ and parents (Polish Zloty [PLN] at 2013–2014 prices) for managing infants over 18 months after starting one of the formulas as well as the relative cost-effectiveness of each of the formulas. Results The probability of developing tolerance to cow’s milk by 18 months was higher among infants who were fed eHCF + LGG (0.82) compared with those fed eHCF alone (0.53) or an AAF (0.22). An infant who is initially managed with eHCF + LGG is expected to consume fewer health care resources than infants managed with the other formulas. Hence, the estimated total health care cost incurred by the NFZ for initially feeding infants with eHCF + LGG (PLN 5,693) was less than that of feeding infants with eHCF alone (PLN 7,749) or an AAF (PLN 24,333). However, the total cost incurred by parents for initially feeding infants with an AAF (PLN 815) was marginally less than that of feeding with eHCF + LGG (PLN 993), which was less than that of feeding with eHCF alone (PLN 1,226). Conclusion Using eHCF + LGG instead of eHCF alone or an AAF for first-line management of newly diagnosed infants with cow’s milk allergy affords a cost-effective use of NFZ-funded resources, since it improves outcome for less cost. Whether eHCF + LGG would be viewed as being cost-effective by parents is dependent on their willingness to pay an additional cost for additional tolerance acquisition to cow’s milk. PMID:27418845

  18. Two-Column Aerosol Project (TCAP) Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Berg, Larry K [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-05-01

    This study included the deployment of the Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility’s Mobile Facility (AMF), ARM Mobile Aerosol Observing System (MAOS) and the ARM Aerial Facility (AAF). The study was a collaborative effort involving scientists from DOE national laboratories, NOAA, NASA, and universities. The AAF and MAOS were deployed for two approximately month-long Intensive Operational Periods (IOPs) conducted in June 2012 and February 2013. Seasonal differences in the aerosol chemical and optical properties observed using the AMF, AAF, and MAOS are presented in this report. The total mass loading of aerosol is found to be much greater in the summer than in the winter, with the difference associated with greater amounts of organic aerosol. The mass fraction of organic aerosol is much reduced in the winter, when sulfate is the dominant aerosol type. Surprisingly, very little sea-salt aerosol was observed in the summer. In contrast, much more sea salt aerosol was observed in the winter. The mass loading of black carbon is nearly the same in both seasons. These differences lead to a relative increase in the aerosol light absorption in the winter and an associated decrease in observed single-scattering albedo. Measurements of aerosol mixing state were made using a single-particle mass spectrometer, which showed that the majority of the summertime aerosol consisted of organic compounds mixed with various amounts of sulfate. A number of other findings are also summarized in the report, including: impact of aerosol layers aloft on the column aerosol optical depth; documentation of the aerosol properties at the AMF; differences in the aerosol properties associated with both columns, which are not systematic but reflect the complicated meteorological and chemical processes that impact aerosol as it is advected away from North America; and new instruments and data-processing techniques for measuring both aerosol and

  19. 游离氨基酸配方粉应用于婴儿湿疹的临床观察

    Institute of Scientific and Technical Information of China (English)

    顾丽; 周慧恩

    2013-01-01

    Objective To investigate the free amino acid formula powder (AAF) on infantile eczema clinical prevention and milk protein allergy (CMA) of infant growth and development impact. Methods From May 2012 to February 2013 the child care center treatment of eczema age 2~6 months of artificial feeding. 70 cases were randomly divided into normal infant group (CMF) in 48 cases and free amino acid formula group (AAF) in 22 cases, avoid milk, soy products, in June Toseuchi Fukasuke food. On two groups of infants intervention for second weeks, 4 weeks, 12 weeks visiting, body weight monitoring, sleep time survey and peripheral blood. Results In AAF group, 18 cases of eczema relieved 2 weeks. 4 weeks, 20 cases of eczema in complete remission, sleep time increased an average of 1.5 hours/day, weight average growth of 0.8 kg /4 week. 12 weeks without recurrence in 19 cases of eczema, sleep an average of 16.3 hours / day, average weight gain of 0.76 kg /4 weeks, peripheral blood eosinophils <5%21 cases. Conclusion AAF can continue to ease the baby eczema, support CMA of infant growth and development. Eczema baby recommend the use of free amino acid formula powder.%  目的探讨游离氨基酸配方粉(AAF)对婴儿湿疹的临床防治及牛奶蛋白过敏(CMA)婴儿的生长发育的影响.方法选择2012年5月~2013年2月儿童保健中心就诊的患湿疹的年龄2~6月龄人工喂养儿70例随机分为普通婴儿配方组(CMF)48例和游离氨基酸配方组(AAF)22例,回避奶、大豆制品,6月龄内不添加辅食.对两组婴儿干预第2周,4周,12周进行访视,体重监测,睡眠时间调查及外周血检测.结果 AAF组2周18例湿疹明显缓解.4周20例湿疹完全缓解,睡眠时间平均提高1.5 h/d,体重平均增长0.8 kg/4周.12周19例湿疹无复发,睡眠时间平均16.3 h/d,体重平均增重0.76 kg/4周,外周血嗜酸性细胞<5%21例.结论 AAF可以持续缓解婴儿湿疹,支持CMA婴儿的生长发育.湿疹婴儿建议使用游离氨基酸配方粉.

  20. Which type of forest management provides most ecosystem services?

    Directory of Open Access Journals (Sweden)

    Timo Pukkala

    2016-04-01

    Full Text Available Background: Forest ecosystems are increasingly seen as multi-functional production systems, which should provide, besides timber and economic benefits, also other ecosystem services related to biological diversity, recreational uses and environmental functions of forests. This study analyzed the performance of even-aged rotation forest management (RFM, continuous cover forestry (CCF and any-aged forestry (AAF in the production of ecosystem services. AAF allows both even-aged and uneven-aged management schedules. The ecosystem services included in the analyses were net present value, volume of harvested timber, cowberry and bilberry yields, scenic value of the forest, carbon balance and suitability of the forest to Siberian jay. Methods: Data envelopment analysis was used to derive numerical efficiency ratios for the three management systems. Efficiency ratio is the sum of weighted outputs (ecosystem services divided by the sum of weighted inputs. The linear programing model proposed by Charnes, Cooper and Rhodes was used to derive the weights for calculating efficiency scores for the silvicultural systems. Results and conclusions: CCF provided more ecosystem services than RFM, and CCF was more efficient than RFM and AAF in the production of ecosystem services. Multi-objective management provided more ecosystem services (except harvested timber than single-objective management that maximized economic profitability. The use of low discount rate (resulting in low cutting level and high growing stock volume led to better supply of most ecosystems services than the use of high discount rate. RFM where NPV was maximized with high discount rate led to particularly poor provision of most ecosystem services. In CCF the provision of ecosystem services was less sensitive to changes in discount rate and management objective than in RFM. Keywords: Data envelopment analysis, Production efficiency, Multi-objective management, Multi-functional forestry

  1. Characterization and enrichment of hepatic progenitor cells in adult rat liver

    Institute of Scientific and Technical Information of China (English)

    Ai-Lan Qin; Xia-Qiu Zhou; Wei Zhang; Hong Yu; Qin Xie

    2004-01-01

    AIM: To detect the markers of oval cells in adult rat liver and to enrich them for further analysis of characterization in vitro.METHODS: Rat model for hepatic oval cell proliferation was established with 2-acetylaminofluorene and two third partial hepatectomy (2-AAF/PH). Paraffin embedded rat liver sections from model (11 d after hepatectomy) and control groups were stained with HE and OV6, cytokeratin19 (CK19),albumin, alpha fetoprotein (AFP), connexin43, and c-kit antibodies by immunohistochemistry. Oval cell proliferation was measured with BrdU incorporation test. C-kit positive oval cells were enriched by using magnetic activated cell sorting (MACS) .The sorted oval cells were cultured in a low density to observe colony formation and to examine their characterization in vitroby immunocytochemistry and RT-PCR. RESULTS: A 2-AAF/PH model was successfully established to activate the oval cell compartment in rat liver. BrdU incorporation test of oval cell was positive. The hepatic oval cells coexpressed oval cell specific marker OV6, hepatocytemarker albumin and cholangiocyte-marker CK19. They also expressed AFP and connexin 43. C-kit, one hematopoietic stem cell receptor, was expressed in hepatic oval cells at high levels. By using c-kit antibody in conjunction with MACS,we developed a rapid oval cell isolation protocol. The sorted cells formed colony when cultured in vitro. Cells in the colony expressed albumin or CK19 or coexpressed both and BrdU incorporation test was positive. RT-PCR on colony showed expression of albumin and CK19 gene.CONCLUSION: Hepatic oval cells in the 2-AAF/PH model had the properties of hepatic stem/progenitor cells. Using MACS, we established a method to isolate oval cells. The sorted hepatic oval cells can form colony in vitro which expresses different combinations of phenotypic markers and genes from both hepatocytes and cholangiocyte lineage.

  2. Parvalbumin immunoreactivity in the auditory cortex of a mouse model of presbycusis.

    Science.gov (United States)

    Martin del Campo, H N; Measor, K R; Razak, K A

    2012-12-01

    Age-related hearing loss (presbycusis) affects ∼35% of humans older than sixty-five years. Symptoms of presbycusis include impaired discrimination of sounds with fast temporal features, such as those present in speech. Such symptoms likely arise because of central auditory system plasticity, but the underlying components are incompletely characterized. The rapid spiking inhibitory interneurons that co-express the calcium binding protein Parvalbumin (PV) are involved in shaping neural responses to fast spectrotemporal modulations. Here, we examined cortical PV expression in the C57bl/6 (C57) mouse, a strain commonly studied as a presbycusis model. We examined if PV expression showed auditory cortical field- and layer-specific susceptibilities with age. The percentage of PV-expressing cells relative to Nissl-stained cells was counted in the anterior auditory field (AAF) and primary auditory cortex (A1) in three age groups: young (1-2 months), middle-aged (6-8 months) and old (14-20 months). There were significant declines in the percentage of cells expressing PV at a detectable level in layers I-IV of both A1 and AAF in the old mice compared to young mice. In layers V-VI, there was an increase in the percentage of PV-expressing cells in the AAF of the old group. There were no changes in percentage of PV-expressing cells in layers V-VI of A1. These data suggest cortical layer(s)- and field-specific susceptibility of PV+ cells with presbycusis. The results are consistent with the hypothesis that a decline in inhibitory neurotransmission, particularly in the superficial cortical layers, occurs with presbycusis.

  3. Evaluation of a repeated dose liver micronucleus assay in rats treated with two genotoxic hepatocarcinogens, dimethylnitrosamine and 2-acetylaminofluorene: the possibility of integrating micronucleus tests with multiple tissues into a repeated dose general toxicity study.

    Science.gov (United States)

    Takashima, Rie; Takasawa, Hironao; Kawasako, Kazufumi; Ohyama, Wakako; Okada, Emiko; Narumi, Kazunori; Fujiishi, Yohei; Wako, Yumi; Yasunaga, Katsuaki; Hattori, Akiko; Kawabata, Masayoshi; Nakadate, Kiyoko; Nakagawa, Munehiro; Hamada, Shuichi

    2015-03-01

    As part of a collaborative study by the Collaborative Study Group for Micronucleus Test (CSGMT) of the Mammalian Mutagenicity Study Group (MMS) in the Japanese Environmental Mutagen Society (JEMS), the present study evaluated the effectiveness of the repeated dose liver micronucleus (RDLMN) assay. Two genotoxic hepatocarcinogens, dimethylnitrosamine (DMN) and 2-acetylaminofluorene (2-AAF), were administered orally to male rats (6 weeks old at the initial dosing) once daily for 14 and 28 days to evaluate the micronucleus (MN) inducibility in the liver. In addition, these chemicals were evaluated for MN inducibility in the bone marrow (BM) and gastrointestinal (GI) tract, i.e. glandular stomach and colon of the same animals used in the RDLMN assay. As a result, both chemicals produced positive results in the liver, although a weak positive response was given by 2-AAF. DMN gave negative results in the tissues other than the liver. 2-AAF produced positive responses in the BM and glandular stomach, and a prominent response was particularly observed in the glandular stomach, which is directly exposed to the test chemicals by gavage. The present results suggest that the RDLMN assay is a useful method for detecting genotoxic hepatocarcinogens, and that it is especially effective for evaluating test chemicals, such as DMN, undetectable by the BM and GI tract MN assay. Moreover, the results in this investigation indicate that the use of multiple tissues in the study integrating the MN tests is more effective than using a single tissue, for detection of the MN induction produced by chemical exposure to rats, and helps to determine the characteristics of the test chemicals.

  4. Dietary administration of Nexrutine inhibits rat liver tumorigenesis and induces apoptotic cell death in human hepatocellular carcinoma cells

    Directory of Open Access Journals (Sweden)

    Shamshad Alam

    2015-01-01

    Full Text Available Epidemiological studies suggested that plant-based dietary supplements can reduce the risk of liver cancer. Nexrutine (NX, an herbal extract from Phellodendronamurense, has been shown to have anti-inflammatory, anti-microbial and anti-tumor activities. In the present study, we have shown the anti-tumor potential of NX against Solt-Farber model with elimination of PH, rat liver tumor induced by diethylnitrosoamine (DEN as carcinogen and 2-acetylaminofluorene (2-AAF as co-carcinogen. The elucidation of mechanistic pathways was explored in human liver cancer cells. Dietary intake of NX significantly decreased the cell proliferation and inflammation, as well as increased apoptosis in the liver sections of DEN/2-AAF-treated rats. Moreover, NX (2.5–10 μg/ml exposure significantly decreased the viability of liver cancer cells and modulated the levels of Bax and Bcl-2 proteins levels. NX treatment resulted in increased cytochrome-c release and cleavage of caspases 3 and 9. In addition, NX decreased the expression of CDK2, CDK4 and associated cyclins E1 and D1, while up-regulated the expression of p21, p27 and p53 expression. NX also enhanced phosphorylation of the mitogen-activated protein kinases (MAPKs ERK1/2, p38 and JNK1/2. Collectively, these findings suggested that NX-mediated protection against DEN/2-AAF-induced liver tumorigenesis involves decrease in cell proliferation and enhancement in apoptotic cell death of liver cancer cells.

  5. Effects of different mitogens on intrasplenic liver tissue transplants in comparison to orthotopic liver.

    Science.gov (United States)

    Lupp, Amelie; Lucas, Norma; Tralls, Manuela; Fuchs, Udo; Danz, Manfred

    2003-06-01

    Ectopic liver cell transplants, when compared to orthotopic liver, can serve as a tool to study topic influences on liver cell differentiation, multiplication, function and responsiveness to xenobiotics. The aim of the present study was to evaluate, if characteristic effects of mitogens are exerted in both liver and intrasplenic liver cell transplants in a similar manner. Fetal liver tissue suspensions were transplanted into the spleens of adult male syngenic rats. Four months later, transplant recipients and controls were treated with fluorene (FEN), fluorenone (FON), 2-acetylaminofluorene (AAF), N-nitrosodibenzylamine (NDBA) or the solvent 48 hours before sacrifice. The following parameters were assessed within livers and spleens: mitotic activity of hepatocytes, glycogen content, cytochrome P450 (P450) isoforms expression, P450 mediated monooxygenase functions, tissue content of lipid peroxides (LPO) and of reduced and oxidized glutathione (GSH; GSSG). In both orthotopic livers and intrasplenic transplants FEN, FON or NDBA administration increased the mitotic activity of the hepatocytes. Treatment with the mitogens caused a distinct and characteristic induction of the P450 isoforms expression and of the respective monooxygenase functions in the livers and (with certain differences) also in the transplants. FEN and FON slightly increased, AAF and NDBA reduced liver glycogen content. The latter effect was also seen in the transplants. NDBA administration caused a slight increase in tissue LPO content in livers, but not in spleens. Additionally, AAF or NDBA treatment led to an elevation of liver (but not of spleen) GSH and GSSG concentrations. The results of the present investigation show that characteristic effects of mitogens on orthotopic liver occur with certain differences also in ectopic liver cell transplants.

  6. The burden of musculoskeletal conditions.

    Directory of Open Access Journals (Sweden)

    Clémence Palazzo

    Full Text Available OBJECTIVE: Despite the burden of rheumatic and musculoskeletal diseases (RMDs, these conditions probably deserve more attention from public health authorities in several countries including developed ones. We assessed their contribution to disability. METHODS: Data on disabilities associated with RMDs were extracted from the national 2008-2009 Disability-Health Survey of 29,931 subjects representative of the population in France. We used the core set of disability categories for RMDs of the World Health Organization's International Classification of Functioning, Disability and Health for analysis. Diagnosis and disabilities were self-reported. We assessed the risk of disability associated with RMDs using odds ratios (ORs and the societal impact of RMDs using the average attributable fraction (AAF. RESULTS: Overall 27.7% (about 17.3 million people (95% CI 26.9-28.4% of the population reported having RMDs. The most prevalent RMDs were low back pain (12.5%, 12.1-13.1 and osteoarthritis (12.3%, 11.8-12.7. People reporting osteoarthritis were more disabled in walking (adjusted OR 1.9, 1.7-2.2 than those without. People reporting inflammatory arthritis were more limited in activities of daily living (from 1.4, 1.2-1.8 for walking to 2.1, 1.5-2.9 for moving around. From a societal perspective, osteoarthritis was the main contributor to activity limitations (AAF 22% for walking difficulties. Changing jobs was mainly attributed to neck pain (AAF 13% and low back pain (11.5%. CONCLUSION: RMDs are highly prevalent and significantly affect activity limitations and participation restrictions. More effort is needed to improve care and research in this field.

  7. Alkaline comet assay in liver and stomach, and micronucleus assay in bone marrow, from rats treated with 2-acetylaminofluorene, azidothymidine, cisplatin, or isobutyraldehyde.

    Science.gov (United States)

    Kraynak, A R; Barnum, J E; Cunningham, C L; Ng, A; Ykoruk, B A; Bennet, B; Stoffregen, D; Merschman, M; Freeland, E; Galloway, S M

    2015-07-01

    As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM) initiative international validation study of the in vivo rat alkaline comet assay (comet assay), we examined the ability of the assay to determine the genotoxicity of 2-acetylaminofluorene (AAF), azidothymidine (AZT), cisplatin (CPN), and isobutyraldehyde (IBA) in liver and glandular stomach of male Sprague-Dawley rats. Rats were given oral doses of test compound or control once daily for three days. High dose levels were approximately maximum tolerated doses and were based on preliminary range-finding studies. Tissues were harvested 3h after the final dose (48h after the initial dose). A bone marrow micronucleus assay (MN) was also conducted on the rats treated with AZT, CPN, and IBA. Acute toxic effects of treatment were determined primarily through histomorphologic analysis of liver and stomach but also by body weight and serum liver enzyme changes. The comet assay was conducted on fresh tissue preparations but frozen samples from two studies were also assayed. Statistically significant dose-related differences in comet % DNA in tail were found in liver and stomach for the genotoxin AZT and in liver for the genotoxin CPN, but not in liver or stomach for the non-genotoxin IBA. Statistically significant differences in % DNA in tail were measured in liver for the low and mid dose of the genotoxin AAF, but not the high dose. The comet assays of frozen liver suspensions from CPN- and AAF-treated rats yielded comparable results to the assays of fresh preparations. There were no indications of significant toxicity induced by any treatment. The micronucleus assay was positive for CPN and AZT and negative for IBA. In conclusion, the in vivo comet assay is capable of detecting genotoxic effects of a variety of chemicals and may fill an important role in the genotoxicity test battery.

  8. Expression of Matrilin-2 in the proliferating liver oval cells of rats%大鼠肝卵圆细胞中matrilin-2的表达及其意义

    Institute of Scientific and Technical Information of China (English)

    陈中; 晏建军; 黄亮; 严以群

    2010-01-01

    目的 探讨大鼠肝脏再生过程中肝卵圆细胞matrilin-2的表达及其意义.方法 利用大鼠部分肝切除(PH)或2-乙酰氨基芴灌胃+/部分肝切除(2-AAF/PH)建立大鼠肝脏再生模型,并设正常对照组.应用免疫组织化学和RT-PCR方法研究肝脏组织中matrilin-2表达情况.结果 免疫组化发现正常对照组及PH组肝脏组织中只有极少量的matrilin-2表达位于胆管血管周围及肝窦状隙内,而在2-AAF/PH模型中可见matrilin-2表达位于门静脉周围的肝窦状隙内,RT-PCR发现2-AAF/PH组肝卵圆细胞中matrilin-2mRNA高表达,而PH组及正常对照组成熟肝细胞中无matrilin-2mRNA表达.结论 matrilin-2是肝卵圆细胞在肝脏再生过程中产生的重要的细胞外基质蛋白,与肝卵圆细胞的增殖分化过程有密切关系.

  9. Remarkable heterogeneity displayed by oval cells in rat and mouse models of stem cell-mediated liver regeneration

    DEFF Research Database (Denmark)

    Jelnes, Peter; Santoni-Rugiu, Eric; Rasmussen, Morten

    2007-01-01

    -collidin (DDC) diet; and N-acetyl-paraaminophen (APAP). Reproducibly, oval cells showing reactivity for cytokeratins (CKs), muscle pyruvate kinase (MPK), the adenosine triphosphate-binding cassette transporter ABCG2/BCRP1 (ABCG2), alpha-fetoprotein (AFP), and delta-like protein 1/preadipocyte factor 1 (Dlk....../Pref-1) were induced in rat liver treated according to the AAF/PHx and CDE but not the DDC protocol. In mouse liver, the CDE, DDC, and APAP protocols all induced CKs and ABCG2-positive oval cells. However, AFP and Dlk/Pref-1 expression was rarely detected in oval cells. CONCLUSION: Our results delineate...

  10. NCBI nr-aa BLAST: CBRC-MDOM-01-0295 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MDOM-01-0295 sp|Q9TTC0|ENV_KORV RecName: Full=Envelope glycoprotein; AltName: Full...=Env polyprotein; Contains: RecName: Full=Surface protein; Short=SU; AltName: Full=Glycoprotein 70; Shor...t=gp70; Contains: RecName: Full=Transmembrane protein; Short=TM; AltName: Full=Envelope protein p15E; Contains: RecName: Full...=R-peptide; AltName: Full=p2E; Flags: Precursor gb|AAF15099.1| envelope protein [Phascolarctos cinereus] Q9TTC0 3e-56 38% ...

  11. NCBI nr-aa BLAST: CBRC-MDOM-02-0179 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MDOM-02-0179 sp|Q9TTC0|ENV_KORV RecName: Full=Envelope glycoprotein; AltName: Full...=Env polyprotein; Contains: RecName: Full=Surface protein; Short=SU; AltName: Full=Glycoprotein 70; Shor...t=gp70; Contains: RecName: Full=Transmembrane protein; Short=TM; AltName: Full=Envelope protein p15E; Contains: RecName: Full...=R-peptide; AltName: Full=p2E; Flags: Precursor gb|AAF15099.1| envelope protein [Phascolarctos cinereus] Q9TTC0 2e-65 38% ...

  12. NCBI nr-aa BLAST: CBRC-DMEL-02-0033 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-02-0033 ref|NP_610712.1| CG13189-PA [Drosophila melanogaster] gb|AAM11370.1| LD29234p [Drosophila... melanogaster] gb|AAF58606.2| CG13189-PA [Drosophila melanogaster] emb|CAL26844.1| CG13189 [Drosophila... melanogaster] emb|CAL26845.1| CG13189 [Drosophila melanogaster] emb|CAL26846.1| CG13189 [Drosophila... melanogaster] emb|CAL26847.1| CG13189 [Drosophila melanogaster] emb|CAL26848.1| CG13189 [Drosop...hila melanogaster] emb|CAL26849.1| CG13189 [Drosophila melanogaster] emb|CAL26850.1| CG13189 [Drosophila

  13. NCBI nr-aa BLAST: CBRC-DSIM-02-0032 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-02-0032 ref|NP_610712.1| CG13189-PA [Drosophila melanogaster] gb|AAM11370.1| LD29234p [Drosophila... melanogaster] gb|AAF58606.2| CG13189-PA [Drosophila melanogaster] emb|CAL26844.1| CG13189 [Drosophila... melanogaster] emb|CAL26845.1| CG13189 [Drosophila melanogaster] emb|CAL26846.1| CG13189 [Drosophila... melanogaster] emb|CAL26847.1| CG13189 [Drosophila melanogaster] emb|CAL26848.1| CG13189 [Drosop...hila melanogaster] emb|CAL26849.1| CG13189 [Drosophila melanogaster] emb|CAL26850.1| CG13189 [Drosophila

  14. NCBI nr-aa BLAST: CBRC-DYAK-02-0031 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-02-0031 ref|NP_610712.1| CG13189-PA [Drosophila melanogaster] gb|AAM11370.1| LD29234p [Drosophila... melanogaster] gb|AAF58606.2| CG13189-PA [Drosophila melanogaster] emb|CAL26844.1| CG13189 [Drosophila... melanogaster] emb|CAL26845.1| CG13189 [Drosophila melanogaster] emb|CAL26846.1| CG13189 [Drosophila... melanogaster] emb|CAL26847.1| CG13189 [Drosophila melanogaster] emb|CAL26848.1| CG13189 [Drosop...hila melanogaster] emb|CAL26849.1| CG13189 [Drosophila melanogaster] emb|CAL26850.1| CG13189 [Drosophila

  15. Compound list: acetamidofluorene [Open TG-GATEs

    Lifescience Database Archive (English)

    Full Text Available acetamidofluorene AAF 00144 ftp://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/...Human/in_vitro/acetamidofluorene.Human.in_vitro.Liver.zip ftp://ftp.biosciencedbc.jp/archive/open-tggates/LA...TEST/Rat/in_vitro/acetamidofluorene.Rat.in_vitro.Liver.zip ftp://ftp.biosciencedbc.jp/archive/open-tggates/L...ATEST/Rat/in_vivo/Liver/Single/acetamidofluorene.Rat.in_vivo.Liver.Single.zip ftp...://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/Rat/in_vivo/Liver/Repeat/acetamidofluorene.Rat.in_vivo.Liver.Repeat.zip ...

  16. NCBI nr-aa BLAST: CBRC-MDOM-02-0202 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MDOM-02-0202 sp|Q9TTC0|ENV_KORV RecName: Full=Envelope glycoprotein; AltName: ...Full=Env polyprotein; Contains: RecName: Full=Surface protein; Short=SU; AltName: Full=Glycoprotein 70; Short=gp70; Contains: RecName...: Full=Transmembrane protein; Short=TM; AltName: Full=Envelope protein p15E; Contains: RecName...: Full=R-peptide; AltName: Full=p2E; Flags: Precursor gb|AAF15099.1| envelope protein [Phascolarctos cinereus] Q9TTC0 8e-65 35% ...

  17. NCBI nr-aa BLAST: CBRC-RNOR-04-0073 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0073 ref|NP_005622.1| smoothened [Homo sapiens] sp|Q99835|SMO_HUMAN Smoothened... homolog precursor (SMO) (Gx protein) gb|AAB41788.1| smoothened [Homo sapiens] gb|AAC24863.1| Gx pro...tein [Homo sapiens] gb|AAD17202.1| smoothened [Homo sapiens] gb|AAF31757.1| Gx protein [Homo sapiens] gb|AAH09989.1| Smoothened... homolog (Drosophila) [Homo sapiens] gb|EAL24102.1| smoothened... homolog (Drosophila) [Homo sapiens] gb|EAW83715.1| smoothened homolog (Drosophila) [Homo sapiens] gb|ABM87646.1| smoothened

  18. NCBI nr-aa BLAST: CBRC-HSAP-07-0071 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-07-0071 ref|NP_005622.1| smoothened [Homo sapiens] sp|Q99835|SMO_HUMAN Smoothened... homolog precursor (SMO) (Gx protein) gb|AAB41788.1| smoothened [Homo sapiens] gb|AAC24863.1| Gx pro...tein [Homo sapiens] gb|AAD17202.1| smoothened [Homo sapiens] gb|AAF31757.1| Gx protein [Homo sapiens] gb|AAH09989.1| Smoothened... homolog (Drosophila) [Homo sapiens] gb|EAL24102.1| smoothened... homolog (Drosophila) [Homo sapiens] gb|EAW83715.1| smoothened homolog (Drosophila) [Homo sapiens] gb|ABM87646.1| smoothened

  19. NCBI nr-aa BLAST: CBRC-OLAT-23-0009 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-23-0009 ref|NP_005622.1| smoothened [Homo sapiens] sp|Q99835|SMO_HUMAN Smoothened... homolog precursor (SMO) (Gx protein) gb|AAB41788.1| smoothened [Homo sapiens] gb|AAC24863.1| Gx pro...tein [Homo sapiens] gb|AAD17202.1| smoothened [Homo sapiens] gb|AAF31757.1| Gx protein [Homo sapiens] gb|AAH09989.1| Smoothened... homolog (Drosophila) [Homo sapiens] gb|EAL24102.1| smoothened... homolog (Drosophila) [Homo sapiens] gb|EAW83715.1| smoothened homolog (Drosophila) [Homo sapiens] gb|ABM87646.1| smoothened

  20. EST Table: FS921386 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS921386 E_FL_fwgP_01I18_F_0 10/09/28 97 %/134 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  1. EST Table: FS764644 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS764644 E_FL_fcaL_37H05_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  2. EST Table: FS842989 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS842989 E_FL_fner_16M03_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  3. EST Table: FY022596 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY022596 bmte3n16 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  4. EST Table: FS928196 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS928196 E_FL_fwgP_21J11_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  5. EST Table: FY746893 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY746893 E_FL_famL_31P20_F_0 11/11/04 96 %/145 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  6. EST Table: FS921370 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS921370 E_FL_fwgP_01I01_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  7. EST Table: FS854752 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS854752 E_FL_fner_50B01_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  8. EST Table: BP183201 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BP183201 NRPG1564 10/09/28 96 %/138 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  9. EST Table: BP182611 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BP182611 NRPG0830 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  10. EST Table: FS770740 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS770740 E_FL_fcaL_20C19_F_0 10/09/28 96 %/126 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  11. EST Table: FS724687 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS724687 E_FL_bmmt_02I04_F_0 10/09/28 95 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  12. EST Table: FY037430 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY037430 rbmte15e13 11/11/04 95 %/140 aa ref|NP_731941.1| effete [Drosophila melano...gaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae s...igase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_...Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA...44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  13. EST Table: BP116656 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BP116656 brS-0376 10/09/28 96 %/137 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  14. EST Table: FS914458 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS914458 E_FL_fufe_32F14_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  15. EST Table: FS910892 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS910892 E_FL_fufe_21J10_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  16. EST Table: FY001836 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY001836 bmov6c22 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  17. EST Table: FY007508 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY007508 bmov22a23 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanog...aster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae st...gase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_0...ull=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA4...4453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  18. EST Table: FS888901 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS888901 E_FL_ftes_43M06_F_0 10/09/28 96 %/129 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  19. EST Table: CK564115 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK564115 rswpb0_007532.y1 10/09/29 95 %/147 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  20. EST Table: FS926059 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS926059 E_FL_fwgP_15D23_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  1. EST Table: FY002576 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY002576 bmov8e12 11/11/04 96 %/140 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  2. EST Table: DC546726 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DC546726 E_FL_phe-_08B12_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  3. EST Table: FS934542 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS934542 E_FL_fwgP_40F12_F_0 10/09/28 96 %/132 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  4. EST Table: FS730301 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS730301 E_FL_bmmt_18H21_F_0 10/09/28 96 %/137 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  5. EST Table: FS922206 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS922206 E_FL_fwgP_03P08_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  6. EST Table: FS921934 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS921934 E_FL_fwgP_03C18_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  7. EST Table: DC439132 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DC439132 E_FL_epM-_26M03_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  8. EST Table: FS767178 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS767178 E_FL_fcaL_45D05_F_0 10/09/28 95 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  9. EST Table: FY746477 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY746477 E_FL_famL_30H07_F_0 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  10. EST Table: FS844631 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS844631 E_FL_fner_21F09_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  11. EST Table: BP183034 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BP183034 NRPG1355 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  12. EST Table: DC549481 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DC549481 E_FL_phe-_17O18_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  13. EST Table: CK561889 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK561889 rswpb0_004790.y1 10/09/29 95 %/147 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  14. EST Table: FS907418 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS907418 E_FL_fufe_11C03_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  15. EST Table: CK515926 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK515926 rswjb0_003472.y1 10/09/29 98 %/114 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  16. EST Table: FS731357 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS731357 E_FL_bmmt_30H20_F_0 10/09/28 96 %/132 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  17. EST Table: CK545790 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK545790 rswhb0_016945.y1 10/09/29 98 %/115 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  18. EST Table: BJ984989 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BJ984989 E_FL_mxg-_15D09_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  19. EST Table: FS815382 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS815382 E_FL_fmgV_45L03_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  20. EST Table: CK540988 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK540988 rswhb0_004772.y1 10/09/29 96 %/147 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  1. EST Table: FY002588 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY002588 bmov8e24 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanoga...ster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae str...ase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_00...ll=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44...453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  2. EST Table: FS913089 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS913089 E_FL_fufe_28C17_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  3. EST Table: CN376997 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CN376997 rzhswab0_005805 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophila m...elanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gamb...ein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] re...ame: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete em...b|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  4. EST Table: DC549650 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DC549650 E_FL_phe-_18I10_F_0 10/09/28 96 %/141 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  5. EST Table: FS837861 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS837861 E_FL_fner_02F03_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  6. EST Table: FS761384 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS761384 E_FL_fcaL_17F15_F_0 10/09/28 95 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  7. EST Table: FS773230 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS773230 E_FL_fcaL_27N20_F_0 10/09/28 96 %/126 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  8. EST Table: FS928060 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS928060 E_FL_fwgP_21D05_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  9. EST Table: FY031713 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY031713 bmte29g06 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanog...aster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae st...gase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_0...ull=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA4...4453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  10. EST Table: FS793905 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available ic process) 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanogaster] ref|NP_001119686.1| effet... (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_001605481.1| PREDICTED: similar t...biquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA44453....1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effete [Drosophila melanogaster] gb...1| ACYPI000078 [Acyrthosiphon pisum] gb|EEZ99199.1| effete [Tribolium castaneum

  11. EST Table: FY003211 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY003211 bmov10a13 11/11/04 96 %/130 aa ref|NP_731941.1| effete [Drosophila melanog...aster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae st...gase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_0...ull=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA4...4453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  12. EST Table: FY744721 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY744721 E_FL_famL_24O14_F_0 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  13. EST Table: BY923442 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BY923442 E_EL_ovS0_29G01_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  14. EST Table: FY026679 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY026679 bmte15e13 11/11/04 95 %/140 aa ref|NP_731941.1| effete [Drosophila melanog...aster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae st...gase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_0...ull=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA4...4453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  15. EST Table: FS855337 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS855337 E_FL_fner_51L11_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  16. EST Table: FS731992 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS731992 E_FL_bmmt_25E22_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  17. EST Table: FS778561 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS778561 E_FL_fcaL_28A16_R_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  18. EST Table: BB989849 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BB989849 E_ET_psgV_29E04_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  19. EST Table: FS905112 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS905112 E_FL_fufe_04A19_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  20. EST Table: FY742279 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY742279 E_FL_famL_17J07_F_0 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  1. EST Table: FS882093 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS882093 E_FL_ftes_23E12_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  2. EST Table: CK559299 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK559299 rswpb0_001251.y1 10/09/29 94 %/147 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  3. EST Table: FS790966 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS790966 E_FL_ffbm_05G22_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  4. EST Table: FY744371 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY744371 E_FL_famL_23O08_F_0 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  5. EST Table: FS768239 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS768239 E_FL_fcaL_48G23_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  6. EST Table: CK559439 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK559439 rswpb0_001478.y1 10/09/29 93 %/146 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  7. EST Table: FS933726 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS933726 E_FL_fwgP_37O22_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  8. EST Table: FS916471 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS916471 E_FL_fufe_38G19_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  9. EST Table: FS846106 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS846106 E_FL_fner_25J11_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  10. EST Table: FS841812 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS841812 E_FL_fner_13H05_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  11. EST Table: FY025849 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY025849 bmte12p03 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophila melanog...aster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae st...gase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_0...ull=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA4...4453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  12. EST Table: CK492797 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK492797 rswab0_011746.y1 10/09/29 96 %/134 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  13. EST Table: FS845554 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS845554 E_FL_fner_24A05_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  14. EST Table: FS930619 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS930619 E_FL_fwgP_28L15_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  15. EST Table: FS760205 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS760205 E_FL_fcaL_13K08_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  16. EST Table: CK563612 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK563612 rswpb0_006842.y1 10/09/29 95 %/147 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  17. EST Table: CK538872 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK538872 rswhb0_001272.y1 10/09/29 96 %/147 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  18. EST Table: FS928407 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS928407 E_FL_fwgP_22C24_F_0 10/09/28 95 %/101 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  19. EST Table: CK538925 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK538925 rswhb0_001332.y1 10/09/29 95 %/145 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  20. EST Table: FS762613 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS762613 E_FL_fcaL_31C14_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  1. EST Table: FS848137 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS848137 E_FL_fner_31G19_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  2. EST Table: FS732667 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS732667 E_FL_bmmt_27C17_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  3. EST Table: FS844536 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS844536 E_FL_fner_21B05_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  4. EST Table: FS919626 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS919626 E_FL_fufe_47O19_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  5. EST Table: FS761611 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS761611 E_FL_fcaL_28A16_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  6. EST Table: FS906757 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS906757 E_FL_fufe_09C11_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  7. EST Table: FS911376 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS911376 E_FL_fufe_23A20_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  8. EST Table: FS930718 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS930718 E_FL_fwgP_29A09_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  9. EST Table: CK490163 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK490163 rswab0_008141.y1 10/09/29 96 %/135 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  10. EST Table: DC543892 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DC543892 E_FL_dpe-_23H20_F_0 10/09/28 96 %/141 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  11. EST Table: FS799832 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS799832 E_FL_fmgV_02F05_F_0 10/09/28 93 %/133 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  12. Modeling RDX Reduction within Iron Bed Reactors

    Science.gov (United States)

    2013-09-01

    a) of an individual particle and volume of water (Vw) along with the definition of void fraction or porosity. This relationship is ( )6 1s w p aa...f = fraction of domain that is mobile or m/ where m is the mobile or effective porosity x = distance along the longitudinal axis of the...upwind differencing for advection (the third term on the right-hand side of Equation 25). A central difference was used for the dispersion term (the

  13. General Plan-Based Environmental Impact Analysis Process Environmental Assessment Volume 1

    Science.gov (United States)

    2009-09-01

    Base, Florida September 2009 iii ACRONYMS AND ABBREVIATIONS % percent °F degrees Fahrenheit µg/m3 micrograms per cubic meter AAFES Army and...Storage Shed 2011 449 0 12,710 (0.29) (0.33) Demolition CE Storage Shed 2011 450 0 12,710 (0.29) (0.21) Demolition CE Hazard Storage/Shed 2011 451 0...mean temperature for Tyndall AFB is 69 degrees Fahrenheit (°F). The average mean temperature during the summer months is 81°F, with record extremes

  14. Main: 1H1Y [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1H1Y イネ Rice Oryza sativa L. D-Ribulose-5-Phosphate 3-Epimerase Oryza Sativa Molecu...V. 326 127 2003 3-Epimerase, Oxidative Pentose Phosphate Pathway, Isomerase SWS:Q9SE42,Q9SE42|EMBL; AF189365; AAF01048.1; -.|PDB; 1H...1Y; X-ray; A/B=1-228.|PDB; 1H1Z; X-ray; A/B=1-228.|Gramene; Q9SE42; -.|GO; GO:001685...ELIQSIKAKGMRPGVSLRPGTPVEEVFPLVEAENPVELVLVMTVEPGFGGQKFMPEMMEKVRALRKKYPSLDIEVDGGLGPSTIDVAASAGANCIVAGSSIFGAAEPGEVISALRKSVEGSQNKS rice_1H1Y.jpg ...

  15. Estudio de una cohorte de pacientes con trombosis de vena retiniana

    OpenAIRE

    Rosa Salazar, Vladimir

    2012-01-01

    Objetivos: analizar la eficacia de una serie de pruebas complementarias realizadas en pacientes con TVR, comparando nuestros resultados con los de la literatura y con un grupo control. Material y Métodos: se han estudiado a 50 pacientes con TVR y 50 pacientes control. Al grupo TVR se les realizó historia clínica exhaustiva, analítica completa, anticuerpos antifosfolípidos (AAF), electrocardiograma (ECG) y ecografía de troncos supraaórticos (TSA). A los controles se les realizó el mismo es...

  16. Multiple Microcomputer Control Algorithm.

    Science.gov (United States)

    1979-09-01

    INDEX, , EAADDOf - LUPO -CZKRO FLAG$ LAPP duFF JUIORD699 2170 0044 0@tlFPOIGOOE00oo00oooSO0o4000 ulISA ALU FLAG$ RCAILWORD CONY 2100 0048...5. E.R(YLL*~~IOFLAGS r6TCH CONY 13.0 aOCI 0t0000000(0006000743C19SCoo AaLU*C.P.C .A.A.f - LUPO -CURO rLAOS.AI.L LAWS CONY 3370 ALU.A(O.V, OUR -4 40.A0

  17. A Real Time Experimental Set Up to Analyse Automatic Actuation of a Fire Sprinkler Using a Shape Memory Alloy (NiTinol)

    OpenAIRE

    Mathew, Y. Ras; Babu, B. Ganesh

    2015-01-01

    A fire sprinkler system has to detect fire and actuate the sprinklers to discharge water automatically to put out the fire as soon as possible. Currently, when a fusible bulb fire sprinkler is exposed to fire, it opens and discharges water automatically but it is not able to stop the discharge of water when the fire is putout. This paper describes a newly designed Automatically Actuated Fire Sprinkler (AAFS) integrated with a NiTinol spring in which the Shape Memory Effect (SME) is utilized t...

  18. Gene : CBRC-CJAC-01-0968 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0968 UN A Chemokines and chemotactic factors receptors CCR5_CALHU 0.0 ...99% gb|AAF87984.1|AF252554_1 CC chemokine receptor 5 [Callithrix jacchus] gb|AAK43361.1|AF177878_1 C-C chemo...kine receptor 5 [Callithrix jacchus] gb|AAN14530.1|AF452614_1 chemokine receptor CCR5 [Callithrix jacchus] g...b|AAQ20011.1| CC chemokine receptor 5 [Callithrix jacchus] 0.0 100% MDYQVSSPIYDID

  19. Procedures, Requirements and Challenges Associated with Analysis of Environmental Samples for Chemical Warfare Material (CWM)

    Science.gov (United States)

    2012-03-29

    TN • Edwards AFB , CA • Ogden, UT • Ft. McClellan , AL • Camp Sibert, AL • Brooksville AAF, FL • Cleveland Plant, OH • Holloman AFB , NM • Lowry BGR...0.064) 19 (0.018) 100 (0.053) Approved for Public Release CONUS Projects • England AFB , LA • Redstone Arsenal, AL • Spring Valley DC • Memphis Depot...Deseret Chemical Depot, UT • Rocky Mt Arsenal, CO • Dover AFB , DE • Bluegrass, KY• Newport, IN • Dugway, UT Approved for Public Release OCONUS Projects

  20. An Evaluation of the Navy Resale System’s Operating and Financial Statements.

    Science.gov (United States)

    1984-03-01

    i-:’ -.- - ;-..:....’. .. :.’.-....’-.’.’..-. .. . - ,- ,-1 1- . ompanies JCPenny Woolworth May Carter AAFES Name NHawley Withheld Type Hale...column for a last year year-to-date percentage of sales. E. BRANCH STORE REPORTS The J. C. Penny Company, F. W. Woolworth Company, and the Army and Air...each period of the year that is completed. 2. F. W. Woolworth Company The F. W. Woolworth Company’s operating state- ment, Exhibit 3-10, is made up of

  1. EST Table: FY008691 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY008691 bmov25g06 11/11/04 95 %/143 aa ref|NP_731941.1| effete [Drosophila melanog...aster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae st...gase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_0...ull=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA4...4453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  2. EST Table: BP179876 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BP179876 ovS308A12f 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophila melano...gaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gambiae s...igase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] ref|XP_...Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete emb|CAA...44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  3. EST Table: CK487304 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CK487304 rswab0_004459.y1 10/09/29 96 %/147 aa ref|NP_731941.1| effete [Drosophila ...melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles gam...tein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum] r...Name: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effete e...mb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  4. EST Table: DC546062 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DC546062 E_FL_phe-_05J15_F_0 10/09/28 96 %/142 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  5. EST Table: FY748686 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY748686 E_FL_famL_39M09_F_0 11/11/04 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  6. EST Table: FS797693 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS797693 E_FL_ffbm_25B19_F_0 10/09/28 95 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  7. EST Table: FS724601 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS724601 E_FL_bmmt_02E06_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  8. EST Table: FS928084 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS928084 E_FL_fwgP_21E05_F_0 10/09/28 96 %/147 aa ref|NP_731941.1| effete [Drosophi...la melanogaster] ref|NP_001119686.1| effete [Acyrthosiphon pisum] ref|XP_310998.2| AGAP000145-PA [Anopheles ...protein ligase) (Ubiquitin carrier protein) (Protein effete) [Tribolium castaneum...AltName: Full=Ubiquitin-protein ligase; AltName: Full=Ubiquitin carrier protein; AltName: Full=Protein effet...e emb|CAA44453.1| ubiquitin-conjugating enzyme [Drosophila melanogaster] gb|AAF55093.1| effet

  9. NCBI nr-aa BLAST: CBRC-DRER-26-0538 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DRER-26-0538 ref|NP_229856.1| hemolysin secretion ATP-binding protein, putative [Vibrio cholera...e O1 biovar eltor str. N16961] ref|ZP_01681061.1| hemolysin secretion ATP-binding protein, putative [Vibrio cholera...e V52] ref|ZP_01971238.1| hemolysin secretion ATP-binding protein, putative [Vibrio cholera...e NCTC 8457] ref|ZP_01974256.1| hemolysin secretion ATP-binding protein, putative [Vibrio cholera...e B33] gb|AAF93375.1| hemolysin secretion ATP-binding protein, putative [Vibrio cholera

  10. Alcohol attributable hospitalisations and costs in Ireland, 2000-2004.

    LENUS (Irish Health Repository)

    Martin, J

    2011-05-01

    The aim of this study was to calculate the number and costs of hospital bed-days due to alcohol use in Ireland over the five year period 2000 to 2004. Age and sex specific Irish alcohol-attributable-fractions (AAFs) were developed by combining international risk estimates with Irish consumption data where available; where not available international AAFs were used. These were applied to national datasets to count the number and costs of bed-days wholly caused and prevented by alcohol and that proportion of bed-days that were partially caused and prevented by alcohol. Between 2000 and 2004, alcohol was estimated to have caused 3,428,973 (10.3%) and prevented 529,239 (1.6%) of hospital bed-days, giving a net number of bed-days due to alcohol of 2,899,734 (8.7%). Over this period the hospital inpatient costs attributed to the negative effects of alcohol were 953,126,381 euros, the costs attributed to hospitalisations prevented were 147,968,164 euros; giving net costs of alcohol-attributed bed-days of 805,158,217 euros. Chronic conditions accounted for 3,262,408 (95%) hospital bed-days due to the harmful effects of alcohol. Conditions not wholly due to alcohol accounted for 2,297,412 (67%) hospital bed-days due to the harmful effects of alcohol. The negative impacts of alcohol were greater than previously thought and spread across the whole population.

  11. Induction of a heat shock response (HSP 72) in rat embryos exposed to selected chemical teratogens.

    Science.gov (United States)

    Mirkes, P E; Doggett, B; Cornel, L

    1994-02-01

    A monoclonal antibody to the 72 kD heat shock protein (HSP 72), Western blot analysis and 2-D gel electrophoresis/autoradiography were used to determine whether selected chemical teratogens induced the synthesis and accumulation of HSP 72 in postimplantation rat embryos exposed in vitro. The chemical teratogens studied include N-Acetoxy-2-acetylaminofluorene (N-Ac-AAF), cadmium chloride (CAD), cyclophosphamide (CP), sodium arsenite (AS), and sodium salicylate (SAL). Exposures to test chemicals were selected that produced obvious embryotoxicity characterized by abnormal development and growth retardation. Of the five chemical teratogens studied, AS and SAL induced the synthesis and accumulation of HSP 72 in day 10 rat embryos. The kinetics of HSP 72 accumulation, however, differed between AS- and SAL-treated embryos. Maximal levels of HSP 72 were observed 24 hours after AS exposure and 10 hours after SAL exposure. N-Ac-AAF, CD, and CP induced obvious embryotoxicity; however, none of these chemical teratogens induced HSP 72 at any of the timepoints assayed. Although only a small sample of chemical teratogens was studied, our results suggest that the heat shock response, characterized by the synthesis and accumulation of HSP 72, is not a general biomarker for chemical teratogens.

  12. Encoding of temporal information by timing, rate, and place in cat auditory cortex.

    Directory of Open Access Journals (Sweden)

    Kazuo Imaizumi

    Full Text Available A central goal in auditory neuroscience is to understand the neural coding of species-specific communication and human speech sounds. Low-rate repetitive sounds are elemental features of communication sounds, and core auditory cortical regions have been implicated in processing these information-bearing elements. Repetitive sounds could be encoded by at least three neural response properties: 1 the event-locked spike-timing precision, 2 the mean firing rate, and 3 the interspike interval (ISI. To determine how well these response aspects capture information about the repetition rate stimulus, we measured local group responses of cortical neurons in cat anterior auditory field (AAF to click trains and calculated their mutual information based on these different codes. ISIs of the multiunit responses carried substantially higher information about low repetition rates than either spike-timing precision or firing rate. Combining firing rate and ISI codes was synergistic and captured modestly more repetition information. Spatial distribution analyses showed distinct local clustering properties for each encoding scheme for repetition information indicative of a place code. Diversity in local processing emphasis and distribution of different repetition rate codes across AAF may give rise to concurrent feed-forward processing streams that contribute differently to higher-order sound analysis.

  13. Some ozone advanced oxidation processes to improve the biological removal of selected pharmaceutical contaminants from urban wastewater.

    Science.gov (United States)

    Espejo, Azahara; Aguinaco, Almudena; Amat, Ana M; Beltrán, Fernando J

    2014-01-01

    Removal of nine pharmaceutical compounds--acetaminophen (AAF), antipyrine (ANT), caffeine (CAF), carbamazepine (CRB), diclofenac (DCF), hydrochlorothiazide (HCT), ketorolac (KET), metoprolol (MET) and sulfamethoxazole (SMX)-spiked in a primary sedimentation effluent of a municipal wastewater has been studied with sequential aerobic biological and ozone advanced oxidation systems. Combinations of ozone, UVA black light and Fe(III) or Fe3O4 constituted the chemical systems. During the biological treatment (hydraulic residence time, HRT = 24 h), only AAF and CAF were completely eliminated, MET, SMX and HCT reached partial removal rates and the rest of compounds were completely refractory. With any ozone advanced oxidation process applied, the remaining pharmaceuticals disappear in less than 10 min. Fe3O4 or Fe(III) photocatalytic ozonation leads to 35% mineralization compared to 13% reached during ozonation alone after about 30-min reaction. Also, biodegradability of the treated wastewater increased 50% in the biological process plus another 150% after the ozonation processes. Both untreated and treated wastewater was non-toxic for Daphnia magna (D. magna) except when Fe(III) was used in photocatalytic ozonation. In this case, toxicity was likely due to the ferryoxalate formed in the process. Kinetic information on ozone processes reveals that pharmaceuticals at concentrations they have in urban wastewater are mainly removed through free radical oxidation.

  14. Lesion bypass in yeast cells: Pol η participates in a multi-DNA polymerase process

    Science.gov (United States)

    Bresson, Anne; Fuchs, Robert P.P.

    2002-01-01

    Replication through (6–4)TT and G-AAF lesions was compared in Saccharomyces cerevisiae strains proficient and deficient for the RAD30-encoded DNA polymerase η (Pol η). In the RAD30 strain, the (6–4)TT lesion is replicated both inaccurately and accurately 60 and 40% of the time, respectively. Surprisingly, in a rad30Δ strain, the level of mutagenic bypass is essentially suppressed, while error-free bypass remains unchanged. Therefore, Pol η is responsible for mutagenic replication through the (6–4)TT photoproduct, while another polymerase mediates its error-free bypass. Deletion of the RAD30 gene also reduces the levels of both accurate and inaccurate bypass of AAF lesions within two different sequence contexts up to 8-fold. These data show that, in contrast to the accurate bypass by Pol η of TT cyclobutane dimers, it is responsible for the mutagenic bypass of other lesions. In conclusion, this paper shows that, in yeast, translesion synthesis involves the combined action of several polymerases. PMID:12110599

  15. POLYADHESINS: AN ARMORY OF GRAM--NEGATIVE PATHOGENS FOR PENETRATION THROUGH THE IMMUNE SHIELD

    Directory of Open Access Journals (Sweden)

    V. P. Zav’yalov

    2013-08-01

    Full Text Available The rapid emergence of treatment-resistant bacterial pathogens has become a major threat to public health. The outbreak of new Shiga-toxin–producing Escherichia coli O104H4 infection occured in Germany in 2011 illustrates this problem. To colonize host tissues, pathogenic bacteria express surface adhesive organelles. The German strain uses aggregative adherence fimbriae I (AAF/I to anchor to the intestinal mucosa and induce inflammation. AAF/I belong to the family of chaperone/usher assembled fimbrial polyadhesins. Polyadhesins are functioning as an armory for penetration through the host immune shield. The polyadhesin-binding to the target cells triggers subversive signal by aggregation of host cell receptors that allow pathogens to mislead and evade immune defense. Their binding is orchestrated with the type III secretion system, which is extremally important for bacterial virulency. Polyadhesins also are involved in biofilm formation making bacteria more resistant to immune response. Because of this, the polyadhesins are potential targets for immune countermeasures against bacterial infections, in particular for anti-adhesion therapy with antibodies to polyadhesins as one of alternatives to antibiotic therapy.

  16. A selective tropism of transfused oval cells for liver

    Institute of Scientific and Technical Information of China (English)

    Jian-Zhi Chen; Hai Hong; Jin Xiang; Ling Xue; Guo-Qiang Zhao

    2003-01-01

    AIM: To explore the biological behaviors of hepatic oval cells after transfused into the circulation of experimental animals.METHODS: Oval cells from male SD rat were transfused into the circulation of a female rat which were treated by a 2-AAF/CCl4 program, through caudal vein. Sex-determining gene sry which located on Y chromosome was examined by PCR and in situ hybridization technique in liver, kidney and spleen of the experimental animals, respectively.RESULTS: The results of the cell-transplant experiment showed that the srygene was detectable only in the liver but not in spleen and kidney of the experimental rats, and no signals could be detected in the control animals. It can be also morphologically proved that some exogenous cells had migrated into the parenchyma of the liver and settled there.CONCLUSION: The result means that there are exogenous cells located in the liver of the experimental animal and the localization is specific to the liver. This indicates that some "signal molecules" must exist in the circulation of the rats treated by 2-AAF/CCl4. These "signal molecules" might play an important role in specific localization and differentiation of transfused oval cells.

  17. Comparison of clastogen-induced gene expression profiles in wild-type and DNA repair-deficient Rad54/Rad54B cells

    Directory of Open Access Journals (Sweden)

    van Benthem Jan

    2010-01-01

    Full Text Available Abstract Background Previously we found that Rad54/Rad54B cells are more sensitive towards mitomycin C (MMC as compared to wild-type (WT cells. This difference in sensitivity was absent upon exposure to other clastogens like bleomycin (BLM and γ-radiation. In order to get further insight into possible underlying mechanisms, gene expression changes in WT and Rad54/Rad54B MEFs (mouse embryonic fibroblasts after exposure to the clastogens MMC and BLM were investigated. Exposures of these cells to mutagens (N-ac-AAF and ENU and vehicle were taken as controls. Results Most exposures resulted in an induction of DNA damage signaling and apoptosis genes and a reduced expression of cell division genes in cells of both genotypes. As expected, responses to N-ac-AAF were very similar in both genotypes. ENU exposure did not lead to significant gene expression changes in cells of both genotypes, presumably due to its short half-life. Gene expression responses to clastogens, however, showed a genotype-dependent effect for BLM and MMC. MMC treated Rad54/Rad54B MEFs showed no induction of p53-signaling, DNA damage response and apoptosis as seen for all the other treatments. Conclusion These data support our finding that different types of clastogens exist and that responses to these types depend on the DNA repair status of the cells.

  18. 旋毛虫肌幼虫cDNA文库的免疫筛选及初步分析%Immunoscreening and Analysis of Muscle Larvae cDNA Library of Trichinella spiralis

    Institute of Scientific and Technical Information of China (English)

    付宝权; 原丽红; 张亚兰; 吴秀萍; 李莲瑞; 卢强; 刘明远; 陈启军; P.Boireau

    2004-01-01

    用旋毛虫感染猪血清对旋毛虫肌幼虫cDNA文库进行了免疫筛选,从1.6×105个重组噬茵体中筛选出21个阳性克隆.阳性克隆的测序结果表明:有9个克隆为未曾报道过的新基因;有9个克隆不含有开放阅读框架(ORF),与旋毛虫线粒体DNA同源,编码核糖体大亚基RNA;3个克隆为旋毛虫已知基因,其中克隆MI12,34与Serine protease inhibitor[Trichinella spiralis](AAF63473)同源性为99%,克隆MI42与Hypothetical OFR 17.20[Trichinella spiralis](AAB48489)同源性为99%,与21 kDExcretory/secretory protein[Trichinellapseudospiralis](AAF79206)同源性为90%,这为进一步研究基因重组抗原奠定了基础.

  19. Formulation and sensory evaluation of Prosopis alba (Algarrobo) pulp cookies with increased iron and calcium dialyzabilities.

    Science.gov (United States)

    Bernardi, C; Drago, S; Sabbag, N; Sanchez, H; Freyre, M

    2006-03-01

    Prosopis alba (algarrobo) is an important indigenous specie, which fruits are used as food and feed since ancient times. Cookies containing algarrobo pulp (AP) with increased iron and calcium availabilities were formulated and sensory evaluated. AP is preferred as food ingredient because of its high sugar content and pleasant flavour. Formulated cookies mean proximal composition was 8.9 g/100 g protein, 7.2 g/100 g dietary fiber, 25 g/100 g total sugar, and 18.5 g/100 g crude fat with iron and calcium contents 30 ppm and 340 ppm, respectively. Ascorbic (AA) and citric (CA) acids at different mM acid: mM Fe were added in order to increase mineral availabilities being evaluated by an in vitro method. Those ratios were 5:1 and 10:1 for AA:Fe whereas for CA:Fe were 50:1 and 100:1 and combinations of them. After chosen the best AA:Fe and CA:Fe ratios (5:1 and 50:1, respectively), sensory evaluation with trained sensory panel and a consumer acceptability test with one hundred and seventy untrained judges were carried out. Acceptability test showed that 77.65% of the people ( 50 years old 15.89%) tasting final formulated cookies indicated that they "like very much" or "moderately like" and there were not consumers rejecting them.

  20. Use of ascorbic and citric acids to increase dialyzable iron from vinal (Prosopis ruscifolia) pulp.

    Science.gov (United States)

    Bernardi, C; Freyre, M; Sambucetti, M E; Pirovani, M E

    2004-01-01

    Vinal (Prosopis ruscifolia) is an ecologically important wild leguminous tree that grows spontaneously in Argentine deforested lands, the fruit of which is consumed by humans and animals. Because considerable iron content with low to intermediate availability has been previously reported in vinal pulps, its enhancement would be of interest. Iron availability was determined as iron dialyzability using an in vitro technique. Response surface methodology was used to evaluate iron availability increase after adding ascorbic and/or citric acids to vinal pulp at different mM acid/mM Fe ratios. Those ratios ranged from 0.05:1 to 9.95:1 and from 0.5:1 to 99.5:1 for ascorbic acid/Fe (AA:Fe) and citric acid/Fe (CA:Fe), respectively. The obtained second- and first-order polynomial equations showed that AA:Fe and CA:Fe molar ratios linear terms had a significant effect on iron dialyzability increase (P iron availability to a maximum of 4.6 times. Additional confirmatory experiments were made adding the same organic acids to different vinal pulps and to a traditional cake prepared with vinal pulp called "patay." There were no significant differences (p > 0.05) between predicted values obtained by the model and experimental results.

  1. Relative cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG in managing infants with cow's milk allergy in Poland

    Directory of Open Access Journals (Sweden)

    Guest JF

    2016-06-01

    Full Text Available Julian F Guest,1,2 Diana Weidlich,1 Maciej Kaczmarski,3 Elzbieta Jarocka-Cyrta,4 Natalia Kobelska-Dubiel,5 Agnieszka Krauze,6 Iwona Sakowska-Maliszewska,7 Anna Zawadzka-Krajewska8 1Catalyst Health Economics Consultants, Northwood, Middlesex, 2Faculty of Life Sciences and Medicine, King’s College, London, UK; 3Department of Pediatrics, Gastroenterology, and Allergology, Medical University of Bialystok, Białystok, 4Uniwersytet Warmińsko‑Mazurski, Wydział Nauk Medycznych, Katedra Pediatrii Klinicznej, Olsztyn, 5Department of Pediatric Gastroenterology and Metabolic Diseases, Poznań University of Medical Sciences, Poznań, 6Klinika Pneumonologii i Alergologii Wieku Dziecięcego, Warsaw, 7Poradnia Gastroenterologiczna Centrum Pediatrii, Sosnowiec, 8Department of Pediatric Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland Objective: To estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (eHCF containing the probiotic Lactobacillus rhamnosus GG (eHCF + LGG; Nutramigen LGG as an initial treatment for cow’s milk allergy compared with eHCF alone and amino acid formulas (AAF in Poland from the perspective of the Polish National Health Fund (Narodowy Fundusz Zdrowia [NFZ] and parents. Methods: Decision modeling was used to estimate the probability of cow’s milk allergic infants developing tolerance to cow’s milk by 18 months. The model also estimated the cost to the NFZ and parents (Polish Zloty [PLN] at 2013–2014 prices for managing infants over 18 months after starting one of the formulas as well as the relative cost-effectiveness of each of the formulas. Results: The probability of developing tolerance to cow’s milk by 18 months was higher among infants who were fed eHCF + LGG (0.82 compared with those fed eHCF alone (0.53 or an AAF (0.22. An infant who is initially managed with eHCF + LGG is expected to consume fewer health care resources than infants managed with the other

  2. A study of oval cell proliferation kinetics in the rat 2-acethlaminofluorene/partial hepatectomy model%大鼠2-乙酰胺基芴/部分肝切除模型中卵圆细胞增殖动力学的初步研究

    Institute of Scientific and Technical Information of China (English)

    项帅; 陈孝平; 张伟; 张斌豪; 梁慧芳

    2010-01-01

    目的 研究大鼠肝脏卵圆细胞增殖模型中卵圆细胞增殖率的变化,进一步了解卵圆细胞生物学特性.方法 采用2-乙酰胺基芴/部分肝切除(2-AAF/PH)的方法建立大鼠肝卵圆细胞增殖模型.免疫荧光双标技术检测肝切除术后不同时间点肝脏石蜡切片中卵圆细胞标志物上皮细胞黏附分子和增殖标志物增殖细胞核抗原的共表达情况,对阳性细胞进行定量计数分析;并采用荧光双标和天狼猩红染色观察此过程中肝星状细胞激活和肝脏胶原沉积的情况.结果 术后第2天门静脉区开始出现少量卵圆细胞,到第9天数量达到高峰,此后数量逐步下降.此过程中卵圆细胞的增殖率逐步下降,从第2天的(91.3±1.6)%下降到第12天的(53.6±4.4)%,差异有统计学意义(P<0.01).激活的肝星状细胞一直伴随卵圆细胞增殖并向肝实质延伸,分泌的胶原形成细胞外基质包绕小管样结构的卵圆细胞.结论 2-AAF/PH模型中卵圆细胞数量达到高峰前,其增殖率已经开始逐渐下降.肝星状细胞可能通过分泌细胞外基质和多种因子严格调控卵圆细胞的增殖.%Objective To investigate the changes of oval cell proliferation rate in the rat 2-acethlaminofluorene/partial hepatectomy(2-AAF/PH)model.Methods Livers were collected from 2-AAF/PH rats at different time points after hepatectomy.Paraffin sections were investigated by double immunofluorescent staining with confocal microscopy for oval cell marker epithelial cell adhesion molecule and proliferative index proliferating cell nuclear antigen,or epithelial cell adhesion molecule and alphasmooth muscle actin Deposition of matrix in liver tissue was detected by sirius red staining.Results Response of ductular oval cells could be observed in portal area at 2 days after PH,and the number of oval cells reached its peak at 9 days and then gradually declined.Oval cell proliferation rate decreased from (91.3±1.6)% at 2 days after PH

  3. Synthesis and Properties of High Energetic Compound 3,3'-Dinitro-4,4'-azofuroxan%3,3’-二硝基-4,4’-偶氮氧化呋咱的合成及性能

    Institute of Scientific and Technical Information of China (English)

    何金选; 卢艳华; 雷晴; 曹一林

    2011-01-01

    以丙二酸单酰肼单钾盐为原材料,经硝化和哑硝化反应“一锅法”合成了4-氨基-3-叠氮羰基氧化呋咱(AN-FO),然后通过ANFO合成出3,3 ’-二氨基-4,4’-偶氮氧化呋咱(DAAFO),DAAFO在双氧水/浓硫酸溶液中氧化为DNAFO.用元素分析、IR、MS和DSC-TG对其结构进行了表征.结果表明,丙二酸单肼单钾盐在25~30℃硝化30min,然后在-22±2℃亚硝化60min,生成3,4-二叠氮羰基氧化呋咱(AAF).AAF在80℃环氧六烷/水溶液中异构化重排得到ANFO,DAAFO在30℃经硝酸/硫酸氧化转化为DNAFO.DNAFO的密度为2.002 g/cm3,生成焓为640.30 kJ/mol.%4-Amino-3-azidocarbonylfuroxan (ANFO) was first synthesized via nitration and nitrosation in "one-pot", using malonic acid monohydrazide monopotassium salt as starting material. Then 3, 3'-diamino-4, 4'-azofuroxan (DAAFO)was prepared using ANFO. Finally,DAAFO were directly oxidized to DNAFO by hydrogen peroxide/sul-furic acid solution. The structures of these compounds were characterized by elementary analyses, FTIR, MS and DSC-TG. The reaction condition for ANFO and DNAFO was found to be: malonic acid monohydrazide monopotassium salt was nitrated at 25 - 30℃ for 30 min, and nitrosated at - 22±2℃ for 60 min, in which furoxan-3,4-dicar-boxylic acid diazide (AAF) was synthesized. ANFO was obtained by isomerization of AAF at 80℃ in dioxane/H2O. DNAFO was obtained by oxidating the DAAFO by HNO3/H2SO4 at 30℃. The density and enthalpy of formation of DNAFO are 2. 002 g/cm3 ,and 640. 30 kJ/mol, respectively.

  4. Comet assay evaluation of six chemicals of known genotoxic potential in rats.

    Science.gov (United States)

    Hobbs, Cheryl A; Recio, Leslie; Streicker, Michael; Boyle, Molly H; Tanaka, Jin; Shiga, Atsushi; Witt, Kristine L

    2015-07-01

    As a part of an international validation of the in vivo rat alkaline comet assay (comet assay) initiated by the Japanese Center for the Validation of Alternative Methods (JaCVAM) we examined six chemicals for potential to induce DNA damage: 2-acetylaminofluorene (2-AAF), N-nitrosodimethylamine (DMN), o-anisidine, 1,2-dimethylhydrazine dihydrochloride (1,2-DMH), sodium chloride, and sodium arsenite. DNA damage was evaluated in the liver and stomach of 7- to 9-week-old male Sprague Dawley rats. Of the five genotoxic carcinogens tested in our laboratory, DMN and 1,2-DMH were positive in the liver and negative in the stomach, 2-AAF and o-anisidine produced an equivocal result in liver and negative results in stomach, and sodium arsenite was negative in both liver and stomach. 1,2-DMH and DMN induced dose-related increases in hedgehogs in the same tissue (liver) that exhibited increased DNA migration. However, no cytotoxicity was indicated by the neutral diffusion assay (assessment of highly fragmented DNA) or histopathology in response to treatment with any of the tested chemicals. Therefore, the increased DNA damage resulting from exposure to DMN and 1,2-DMH was considered to represent a genotoxic response. Sodium chloride, a non-genotoxic non-carcinogen, was negative in both tissues as would be predicted. Although only two (1,2-DMH and DMN) out of five genotoxic carcinogens produced clearly positive results in the comet assay, the results obtained for o-anisidine and sodium arsenite in liver and stomach cells are consistent with the known mode of genotoxicity and tissue specificity exhibited by these carcinogens. In contrast, given the known genotoxic mode-of-action and target organ carcinogenicity of 2-AAF, it is unclear why this chemical failed to convincingly increase DNA migration in the liver. Thus, the results of the comet assay validation studies conducted in our laboratory were considered appropriate for five out of the six test chemicals.

  5. 肝细胞生成素Cn促进肝干/祖细胞增殖的功能研究%Effect of hepatopoietin Cn on liver stem cells

    Institute of Scientific and Technical Information of China (English)

    李永锋; 刘勇; 常菁; 刘鹏飞; 高慧英; 周旭; 卢俊; 崔春萍

    2015-01-01

    目的:检测肝细胞刺激因子肝细胞生成素Cn( hepatopoietin Cn,HPPCn)作用肝祖细胞的功能。方法利用MTT法、AnnexinⅤ和PI荧光染色法分别检测WB-F344细胞增殖及其抗凋亡能力,Transwell法检测细胞迁移能力。建立小鼠2AAF-部分肝切除(partial hepatectomy)模型,分析高表达HPPCn对肝干/祖细胞增殖和迁移的影响。结果重组HPPCn蛋白能促进WB-F344细胞的增殖及细胞迁移,低浓度HPPCn能抑制WB-F344细胞的凋亡,并激活WB-F344细胞中SphK1、Erk1/2以及Stat3信号通路。动物模型检测结果显示,肝特异HPPCn转基因小鼠部分肝切除后,肝卵圆细胞较对照组明显增多,肝再生能力增强。结论 HPPCn能促进肝干/祖细胞增殖和存活,并通过促进肝卵圆细胞增生,提高肝再生能力。%Objective To investigate the effect of hepatopoietin Cn(HPPCn) on liver stem cells.Methods In this study, WB-F344 cell line was used, and MTT and flow cytometry assay were conducted to determine cell proliferation and apoptosis.Transwell assay was used to test the migration of WB-F344 cells.A 2AAF-partial hepatectomy(PH) mouse model was used to observe the effect of HPPCn on liver stem cell proliferation in vivo.Results HPPCn enhanced WB-F344 cell proliferation and migration and activated the SphK1, Erk and Stat3 signal pathways.The analysis of the 2AAF-PH mouse model showed that oval cells in the experimental group far outnumbered those in control and the regeneration of the liver was improved post PH.Conclusion HPPCn can increase the liver stem cell proliferation and survival while promoting the regenenation of the liver by augmenting oval cell proliferation.

  6. 龙葵碱对人肝癌HepG2细胞N-乙酰基转移酶活性的影响%Effect of solanine on N-acetyltransferase activity in HepG2 cell

    Institute of Scientific and Technical Information of China (English)

    高世勇; 季字彬

    2008-01-01

    目的 探讨龙葵碱对HepG2细胞Ⅳ-乙酰基转移酶(NAT)活性的影响.方法 采用HPLC方法,以2-氨基芴(2-AF)为底物,以2-AF被NAT乙酰化为2-乙酰氨基芴(2-AAF)的量来反应NAT的活性.结果 龙葵碱能显著降低HepG2完整细胞NAT的活性;龙葵碱能够降低HepG2细胞质内NAT的活性,作用具有剂量依赖性.结论 龙葵碱通过抑制HepG2细胞NAT的活性发挥细胞毒作用.

  7. Sprawozdanie z 65th Anniversary Annual Scientific Meeting of American Academy of Forensic Sciences w Waszyngtonie, USA

    Directory of Open Access Journals (Sweden)

    2014-10-01

    Full Text Available W dniach 18-23.02.2013 roku w Waszyngtonie odbyła się organizowana corocznie konferencja naukowa American Academy of Forensic Sciences (AAFS. Tym razem było to już 65. jubileuszowe spotkanie. Motto konferencji brzmiało: „Nauki sądowe: oparte na obserwacji i doświadczeniu, rozwinięte przez edukację i badania”. Miejscem obrad było centrum konferencyjne w hotelu Marriott Wardman Park, składające się z kilku obszernych sal konferencyjnych mogących łącznie pomieścić około 2000 uczestników, z których około 900 prezentowało swoje prace w formie referatów lub posterów.

  8. OLYMPEX Counterflow Spectrometer and Impactor Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Poellot, Michael [Univ. of North Dakota, Grand Forks, ND (United States)

    2016-07-01

    The U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility’s ARM Aerial Facility (AAF) Counterflow Spectrometer and Impactor (CSI) probe was flown on the University of North Dakota Cessna Citation research aircraft during the Olympic Mountain Experiment (OLYMPEX). The field campaign took place from November 12 through December 19, 2015, over the Olympic Mountains and coastal waters of Washington State as part of a National Aeronautics and Space Administration (NASA) Global Precipitation Measurement (GPM) validation campaign. The CSI was added to the Citation instrument suite to support the NASA Aerosol-Cloud Ecosystem (ACE) satellite program and flights of the NASA Lockheed Earth Resources (ER-2) aircraft. ACE funded extra ER-2 flights to focus on clouds that are weakly precipitating, which are also of interest to the DOE Atmospheric System Research (ASR) program.

  9. NCBI nr-aa BLAST: CBRC-STRI-01-2545 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2545 ref|NP_005622.1| smoothened precursor [Homo sapiens] sp|Q99835|SM...O_HUMAN RecName: Full=Smoothened homolog; Short=SMO; AltName: Full=Gx protein; Flags: Precursor gb|AAB41788.1| smoothened... [Homo sapiens] gb|AAC24863.1| Gx protein [Homo sapiens] gb|AAD17202.1| smoothened [Homo sapien...s] gb|AAF31757.1| Gx protein [Homo sapiens] gb|AAH09989.1| Smoothened homolog (Dr...osophila) [Homo sapiens] gb|EAL24102.1| smoothened homolog (Drosophila) [Homo sapiens] gb|EAW83715.1| smoothened

  10. Precipitation and Hydrology Experiment Counter-Flow Spectrometer and Impactor Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Poellot, Michael [University of North Dakota

    2016-03-01

    The U.S. Department of Energy (DOE)’s Atmospheric Radiation Measurement (ARM) Climate Research Facility Aerial Facility (ARM AAF) counter-flow spectrometer and impactor (CSI) probe was flown on the University of North Dakota Cessna Citation research aircraft during the Integrated Precipitation and Hydrology Experiment (IPHEX). The field campaign took place during May and June of 2014 over North Carolina and its coastal waters as part of a National Aeronautics and Space Administration (NASA) Global Precipitation Measurement validation campaign. The CSI was added to the Citation instrument suite to support the involvement of Jay Mace through the NASA Advanced Composition Explorer (ACE) satellite program and flights of the NASA ER-2 aircraft, which is a civilian version of the Air Force’s U2-S reconnaissance platform. The ACE program funded extra ER-2 flights to focus on clouds that are weakly precipitating, which are also of interest to the Atmospheric System Research program sponsored by DOE.

  11. OPPORTUNICTIC ENTEROBACTERIACAE AS THE CAUSE OF THE ACUTE DIARRHEA AND GUT DISBIOSIS

    Directory of Open Access Journals (Sweden)

    S. A. Egorova

    2011-01-01

    Full Text Available Abstract. Strains of Klebsiella pneumonia isolated from faces were studied to detect virulence factors of “classical” enteric pathogens causing acute diarrhea (Shigella, Salmonella, diarreagenic Escherichia coli. We haven’t detect in Klebsiella the genes encode different virulence factors: the abilities to adherence (sfa, afa, aaf/1, eae, invasion (ipaH, ial, production of heat-lable toxins (elt, LT II, heat-stable toxins (st1, st2 and Shiga toxins (stx1, stx2. Klebsiella spp. is well-known pathogen of the opportunistic infections of urinary tract, blood, wound, respiratory tract. But this bacteria hasn’t virulence factors of diarreagenic Enterobacteriaceae and can’t cause acute diarrhea and other gut pathology.

  12. Dicty_cDB: SSM649 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2 3 BU496934 |BU496934.1 PfESToab60h01.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA ... |BU498261.1 PfESToab94h09.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium f...2 2e-22 3 BI815642 |BI815642.1 PfESToaa31a10.y1 Plasmodium falciparum 3D7 asexual...smodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to SW:R...e cds. 115 2e-31 2 CA758831 |AAF60289.1, mRNA sequence. 64 1e-27 3 BU495396 |BU495396.1 PfESToab72d08.y1 Pla

  13. Binding of the human nucleotide excision repair proteins XPA and XPC/HR23B to the 5R-thymine glycol lesion and structure of the cis-(5R,6S) thymine glycol epimer in the 5′-GTgG-3′ sequence: destabilization of two base pairs at the lesion site

    OpenAIRE

    Brown, Kyle L.; Roginskaya, Marina; Zou, Yue; Altamirano, Alvin; Basu, Ashis K.; Stone, Michael P.

    2009-01-01

    The 5R thymine glycol (5R-Tg) DNA lesion exists as a mixture of cis-(5R,6S) and trans-(5R,6R) epimers; these modulate base excision repair. We examine the 7:3 cis-(5R,6S):trans-(5R,6R) mixture of epimers paired opposite adenine in the 5′-GTgG-3′ sequence with regard to nucleotide excision repair. Human XPA recognizes the lesion comparably to the C8-dG acetylaminoflourene (AAF) adduct, whereas XPC/HR23B recognition of Tg is superior. 5R-Tg is processed by the Escherichia coli UvrA and UvrABC p...

  14. Clinical and echocardiographic features of aorto-atrial fistulas

    Directory of Open Access Journals (Sweden)

    Ananthasubramaniam Karthik

    2005-01-01

    Full Text Available Abstract Aorto-atrial fistulas (AAF are rare but important pathophysiologic conditions of the aorta and have varied presentations such as acute pulmonary edema, chronic heart failure and incidental detection of the fistula. A variety of mechanisms such as aortic dissection, endocarditis with pseudoaneurysm formation, post surgical scenarios or trauma may precipitate the fistula formation. With increasing survival of patients, particularly following complex aortic reconstructive surgeries and redo valve surgeries, recognition of this complication, its clinical features and echocardiographic diagnosis is important. Since physical exam in this condition may be misleading, echocardiography serves as the cornerstone for diagnosis. The case below illustrates aorto-left atrial fistula formation following redo aortic valve surgery with slowly progressive symptoms of heart failure. A brief review of the existing literature of this entity is presented including emphasis on echocardiographic diagnosis and treatment.

  15. Prototype Repository - Sensor data report (period 100917-110101) Report no 24

    Energy Technology Data Exchange (ETDEWEB)

    Goudarzi, Reza [Clay Technology AB, Lund (Sweden)

    2012-08-15

    The Prototype Repository Test consists of two sections. The installation of the first Section of Prototype Repository was made during summer and autumn 2001 and Section 2 was installed in spring and summer 2003. At the end of November 2010 stared the dismantling of the outer section. This report presents data from measurements in the Prototype Repository during the period 2001-09-17-2011-01-01. The report is organized so that the actual measured results are shown in Appendix 1-10, where Appendix 8 deals with measurements of canister displacements (by AITEMIN), Appendix 9 deals with geo-electric measurements in the backfill (by GRS), Appendix 10 deals with stress and strain measurement in the rock (by AaF) and Appendix 11 deals with measurement of water pressure in the rock (by VBB/VIAK). The main report and Appendix 1-7 deal with the rest of the measurements.

  16. Audiometria de altas frequências no diagnóstico complementar em audiologia: uma revisão da literatura nacional High-frequency audiometry in audiological complementary diagnosis: a revision of the national literature

    Directory of Open Access Journals (Sweden)

    Karlin Fabianne Klagenberg

    2011-03-01

    Full Text Available A audiometria de altas frequências (AAF é um exame audiológico importante na detecção precoce de perdas auditivas por lesões na base do ducto coclear. Nos últimos anos, a sua utilização foi facilitada pelo fato de os audiômetros comercializados passarem a incorporar frequências superiores a 8 kHz. Porém, existem diferenças relacionadas aos equipamentos utilizados, às metodologias empregadas e/ou aos resultados e interpretação. Assim, o objetivo deste artigo foi analisar a produção científica nacional sobre a aplicação clínica com AAF, para compreender sua utilização atual. Foram pesquisados textos publicados e indexados nas bases de dados LILACS, SciELO e Medline, num período de tempo de dez anos, utilizando como descritor "audiometria de altas frequências/high-frequency audiometry". Encontraram-se 24 artigos científicos nacionais utilizando AAF, cuja população avaliada, em sua maioria, apresentava de 18 a 50 anos de idade; 13 dos estudos determinaram os limiares utilizando como referência decibel nível de audição (dBNA; alguns estudos realizaram a comparação dos limiares auditivos tonais entre grupos para definir a normalidade; os autores relataram diferenças significativas nos limiares auditivos de altas frequências entre as idades. A AAF é utilizada na clínica audiológica para identificação precoce de alterações auditivas e no acompanhamento da audição de sujeitos expostos a drogas ototóxicas e/ou agentes otoagressores.High-frequency audiometry (HFA is an important audiological test for early detection of hearing losses caused by leasions in the base of the cochlear duct. In recent years, its use was facilitated because audiometers began to identify frequencies higher than 8 kHz. However, there are differences related to the equipment used, the methodologies followed, and/or to the results and their interpretation. Therefore, the aim of this study was to analyze the national scientific production

  17. NCBI nr-aa BLAST: CBRC-CFAM-27-0055 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available te receptor type 2 member 8 (T2R8) (Taste receptor family B member 5) (TRB5) gb|AAF...43907.1|AF227134_1 candidate taste receptor T2R8 [Homo sapiens] gb|AAH69084.1| TAS2R8 protein [Homo sapiens] gb|AAH69551.1| Taste... receptor, type 2, member 8 [Homo sapiens] gb|AAH98353.1| Taste... receptor, type 2, member 8 [Homo sapiens] gb|AAH96701.1| Taste receptor, type 2, member 8 [Homo sapiens] gb|AAH96735.1| Taste...CBRC-CFAM-27-0055 ref|NP_076407.1| taste receptor, type 2, member 8 [Homo sapiens] sp|Q9NYW2|TA2R8_HUMAN Tas

  18. NCBI nr-aa BLAST: CBRC-HSAP-12-0009 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available te receptor type 2 member 8 (T2R8) (Taste receptor family B member 5) (TRB5) gb|AAF...43907.1|AF227134_1 candidate taste receptor T2R8 [Homo sapiens] gb|AAH69084.1| TAS2R8 protein [Homo sapiens] gb|AAH69551.1| Taste... receptor, type 2, member 8 [Homo sapiens] gb|AAH98353.1| Taste... receptor, type 2, member 8 [Homo sapiens] gb|AAH96701.1| Taste receptor, type 2, member 8 [Homo sapiens] gb|AAH96735.1| Taste...CBRC-HSAP-12-0009 ref|NP_076407.1| taste receptor, type 2, member 8 [Homo sapiens] sp|Q9NYW2|TA2R8_HUMAN Tas

  19. Dicty_cDB: Contig-U15709-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available moea batatas... 54 0.022 1 ( AU034760 ) Dictyostelium discoideum slug cDNA, clone SLE286. 54 0.022 1 ( CU595956 ) Theobroma cacao,... mRNA sequence (KZ0AAF2YG05FM1). 54 0.022 1 ( CU585219 ) Theobroma cacao, mRNA sequenc...yte cDNA, clone c-64. 46 5.4 1 ( AM742886 ) Cucumis melo subsp. melo EST, clone 46d_12-B05-M13R. 46 5.4 1 ( ...CU585220 ) Theobroma cacao, mRNA sequence (KZ0AAB4YM24FM1). 46 5.4 1 ( BI783235 ) kh48g09.y1 Ascaris suum ma

  20. Protection by beverages, fruits, vegetables, herbs, and flavonoids against genotoxicity of 2-acetylaminofluorene and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in metabolically competent V79 cells.

    Science.gov (United States)

    Edenharder, R; Sager, J W; Glatt, H; Muckel, E; Platt, K L

    2002-11-26

    Chinese hamster lung fibroblasts, genetically engineered for the expression of rat cytochrome P450 dependent monooxygenase 1A2 and rat sulfotransferase 1C1 (V79-rCYP1A2-rSULT1C1 cells), were utilized to check for possible protective effects of beverages of plant origin, fruits, vegetables, and spices against genotoxicity induced by 2-acetylaminofluorene (AAF) or 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Antigenotoxic activities of juices from spinach and red beets against AAF could be monitored with similar effectivity by the HPRT-mutagenicity test (IC(50)=0.64%; 2.57%) and alkaline single cell gel electrophoresis (comet assay; IC(50)=0.12%; 0.89%) which detects DNA strand breaks and abasic sites. Applying the comet assay, genotoxicity of PhIP could, however, be demonstrated only in the presence of hydroxyurea and 1-[beta-D-arabinofuranosyl]cytosine, known inhibitors of DNA repair synthesis. As expected, AAF and PhIP were unable to induce any genotoxic effects in the parent V79 cells. Genotoxic activity of PhIP was strongly reduced in a dose-related manner by green tea and red wine, by blueberries, blackberries, red grapes, kiwi, watermelon, parsley, and spinach, while two brands of beer, coffee, black tea, rooibos tea, morellos, black-currants, plums, red beets, broccoli (raw and cooked), and chives were somewhat less active. One brand of beer was only moderately active while white wine, bananas, white grapes, and strawberries were inactive. Similarly, genotoxicity of AAF was strongly reduced by green, black, and rooibos tea, red wine, morellos, black-currants, kiwi, watermelon, and spinach while plums, red beets, and broccoli (raw) were less potent. Broccoli cooked exerted only moderate and white wine weak antigenotoxic activity. With respect to the possible mechanism(s) of inhibition of genotoxicity, benzo[a]pyrene-7,8-dihydrodiol (BaP-7,8-OH) and N-OH-PhIP were applied as substrates for the CYP1A family and for rSULT 1C1, respectively. Morellos

  1. Signal analysis and processing for random binary phase coded pulse radar

    Institute of Scientific and Technical Information of China (English)

    孙光民; 刘国岁; 顾红

    2004-01-01

    The application of the random binary phase coded signal in the CW radar system has been limited by the difficulty to isolate the tranmission and reception signal. In order to make use of the random binary phase coded signal, the random binary phase coded pulse radar (RBPC-PR) system has been studied. First, the average ambiguity function (AAF) of the RBPC-PR signal has been analyzed. Then, a statistical method of reducing the range sidelobe (RSL) is presented. Finally, a signal processing scheme of the RBPC-PR is developed. The simulation results show that by using the scheme, the jamming immunity of the system, the resolution and accuracy of distance and velocity have been improved, and the distance and velocity vagueness caused by periods can also be removed. The RSL can be reduced over 30dB by the statistical average method, thus the probability ambiguity caused by random noise can be avoided.

  2. Historical Trends in Graduate Research and Training of Diplomates of the American Board of Forensic Anthropology.

    Science.gov (United States)

    Bethard, Jonathan D

    2017-01-01

    The history of forensic anthropology has been documented by numerous scholars. These contributions have described the work of early pioneers in the field and have described important milestones, such as the founding of the Physical Anthropology Section of the American Academy of Forensic Sciences (AAFS) in 1972 and the American Board of Forensic Anthropology (ABFA) in 1977. This paper contributes to the growing literature on the history of forensic anthropology by documenting the academic training of all individuals who have been granted diplomate status by the ABFA (n = 115). Doctoral dissertation titles were queried to discern broad patterns of research foci. A total of 39 doctoral granting institutions have trained diplomates and 77.3% of board-certified forensic anthropologists wrote dissertations involving skeletal biology, bioarchaeology, or forensic anthropology. Board-certified forensic anthropologists are a broadly trained group of professionals with far-reaching anthropological interests and expertise.

  3. Deeper insights into PCBs in orcas

    DEFF Research Database (Denmark)

    Dietz, Rune; Eulaers, Igor; Desforges, Jean-Pierre;

    2016-01-01

    Having read the recent article by Jepson and Law (DOI: 10.1126/science.aaf9075) we want to emphasise the worrying nature of the fact that at present many marine apex predators, including killer whales, remain highly polluted with PCBs despite world-wide initiatives over past decades to restrict...... the production and use of these compounds. Our research shows that, among a cocktail of industrial pollutants, PCBs are the primary driver of reproductive, immunotoxic and carcinogenic effects. While environmental PCB concentrations were indeed observed declining after legal mitigation, large body burdens remain...... in many top predators, especially in the North Atlantic. Moreover, both intentional and unintentional production of PCBs, as well the use and recycling of PCB-containing equipment, are contemporary primary and secondary sources. The Stockholm Convention therefore urges its ratifying parties to cease using...

  4. NCBI nr-aa BLAST: CBRC-HSAP-10-0036 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-10-0036 ref|NP_000672.2| alpha-2A-adrenergic receptor [Homo sapiens] sp|P...08913|ADA2A_HUMAN Alpha-2A adrenergic receptor (Alpha-2A adrenoceptor) (Alpha-2A adrenoreceptor) (Alpha-2AAR) (Alpha-2 adrenerg...ic receptor subtype C10) gb|AAF91441.1|AF281308_1 alpha 2A adrenergic receptor [Homo sapi...ens] gb|AAG00447.2| adrenergic receptor alpha-2A [Homo sapiens] gb|AAK26743.1| alpha-2A adrenerg...ic receptor [Homo sapiens] gb|AAK51162.1| alpha-2A adrenergic receptor [Homo sapiens] gb|AAH35047.1| Adrenerg

  5. NCBI nr-aa BLAST: CBRC-CJAC-01-0597 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0597 ref|NP_000672.2| alpha-2A-adrenergic receptor [Homo sapiens] sp|P...08913|ADA2A_HUMAN Alpha-2A adrenergic receptor (Alpha-2A adrenoceptor) (Alpha-2A adrenoreceptor) (Alpha-2AAR) (Alpha-2 adrenerg...ic receptor subtype C10) gb|AAF91441.1|AF281308_1 alpha 2A adrenergic receptor [Homo sapi...ens] gb|AAG00447.2| adrenergic receptor alpha-2A [Homo sapiens] gb|AAK26743.1| alpha-2A adrenerg...ic receptor [Homo sapiens] gb|AAK51162.1| alpha-2A adrenergic receptor [Homo sapiens] gb|AAH35047.1| Adrenerg

  6. NCBI nr-aa BLAST: CBRC-FCAT-01-0073 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-0073 ref|NP_000672.2| alpha-2A-adrenergic receptor [Homo sapiens] sp|P...08913|ADA2A_HUMAN Alpha-2A adrenergic receptor (Alpha-2A adrenoceptor) (Alpha-2A adrenoreceptor) (Alpha-2AAR) (Alpha-2 adrenerg...ic receptor subtype C10) gb|AAF91441.1|AF281308_1 alpha 2A adrenergic receptor [Homo sapi...ens] gb|AAG00447.2| adrenergic receptor alpha-2A [Homo sapiens] gb|AAK26743.1| alpha-2A adrenerg...ic receptor [Homo sapiens] gb|AAK51162.1| alpha-2A adrenergic receptor [Homo sapiens] gb|AAH35047.1| Adrenerg

  7. NCBI nr-aa BLAST: CBRC-PTRO-11-0035 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-11-0035 ref|NP_000672.2| alpha-2A-adrenergic receptor [Homo sapiens] sp|P...08913|ADA2A_HUMAN Alpha-2A adrenergic receptor (Alpha-2A adrenoceptor) (Alpha-2A adrenoreceptor) (Alpha-2AAR) (Alpha-2 adrenerg...ic receptor subtype C10) gb|AAF91441.1|AF281308_1 alpha 2A adrenergic receptor [Homo sapi...ens] gb|AAG00447.2| adrenergic receptor alpha-2A [Homo sapiens] gb|AAK26743.1| alpha-2A adrenerg...ic receptor [Homo sapiens] gb|AAK51162.1| alpha-2A adrenergic receptor [Homo sapiens] gb|AAH35047.1| Adrenerg

  8. NCBI nr-aa BLAST: CBRC-PABE-11-0036 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-11-0036 ref|NP_000672.2| alpha-2A-adrenergic receptor [Homo sapiens] sp|P...08913|ADA2A_HUMAN Alpha-2A adrenergic receptor (Alpha-2A adrenoceptor) (Alpha-2A adrenoreceptor) (Alpha-2AAR) (Alpha-2 adrenerg...ic receptor subtype C10) gb|AAF91441.1|AF281308_1 alpha 2A adrenergic receptor [Homo sapi...ens] gb|AAG00447.2| adrenergic receptor alpha-2A [Homo sapiens] gb|AAK26743.1| alpha-2A adrenerg...ic receptor [Homo sapiens] gb|AAK51162.1| alpha-2A adrenergic receptor [Homo sapiens] gb|AAH35047.1| Adrenerg

  9. AcEST: DK948158 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9FHA6|Q9FHA6_ARATH Gb|AAF64534.1 (Putative uncharacterized p... 64 8e-09 tr|Q6TF27|Q6TF27_SOLCH Rapid alkali...ARATH At4g13950 OS=Arabidopsis thaliana PE=4 SV=1 49 3e-04 tr|B6U664|B6U664_MAIZE Rapid... alkalinization factor 1 OS=Zea mays... 49 3e-04 tr|B6TU33|B6TU33_MAIZE Rapid alkalinization factor 1 ...T +C A GPV+PY+RGCS IT C R Sbjct: 81 YISYGALSANRVPCPPRS-GRSYYTHNCFRARGPVHPYSRGCSSITRCRR 129 >tr|Q6TF27|Q6TF27_SOLCH Rapid

  10. Two novel EHEC/EAEC hybrid strains isolated from human infections.

    Directory of Open Access Journals (Sweden)

    Rita Prager

    Full Text Available The so far highest number of life-threatening hemolytic uremic syndrome was associated with a food-borne outbreak in 2011 in Germany which was caused by an enterohemorrhagic Escherichia coli (EHEC of the rare serotype O104:H4. Most importantly, the outbreak strain harbored genes characteristic of both EHEC and enteroaggregative E. coli (EAEC. Such strains have been described seldom but due to the combination of virulence genes show a high pathogenicity potential. To evaluate the importance of EHEC/EAEC hybrid strains in human disease, we analyzed the EHEC strain collection of the German National Reference Centre for Salmonella and other Bacterial Enteric Pathogens (NRC. After exclusion of O104:H4 EHEC/EAEC strains, out of about 2400 EHEC strains sent to NRC between 2008 and 2012, two strains exhibited both EHEC and EAEC marker genes, specifically were stx2 and aatA positive. Like the 2011 outbreak strain, one of the novel EHEC/EAEC harbored the Shiga toxin gene type stx2a. The strain was isolated from a patient with bloody diarrhea in 2010, was serotyped as O59:H-, belonged to MLST ST1136, and exhibited genes for type IV aggregative adherence fimbriae (AAF. The second strain was isolated from a patient with diarrhea in 2012, harbored stx2b, was typed as Orough:H-, and belonged to MLST ST26. Although the strain conferred the aggregative adherence phenotype, no known AAF genes corresponding to fimbrial types I to V were detected. In summary, EHEC/EAEC hybrid strains are currently rarely isolated from human disease cases in Germany and two novel EHEC/EAEC of rare serovars/MLST sequence types were characterized.

  11. RACORO Extended-Term Aircraft Observations of Boundary-Layer Clouds

    Energy Technology Data Exchange (ETDEWEB)

    Vogelmann, A. M.; McFarquhar, Greg; Ogren, John A.; Turner, David D.; Comstock, Jennifer M.; Feingold, G.; Long, Charles N.; Jonsson, Haf; Bucholtz, Anthony; Collins, Donald R.; Diskin, G. S.; Gerber, H.; Lawson, Paul; Woods, Roy; Andrews, Elizabeth; Yang, Hee-Jung; Chiu, Christine J.; Hartsock, Daniel; Hubbe, John M.; Lo, Chaomei; Marshak, A.; Monroe, Justin; McFarlane, Sally A.; Schmid, Beat; Tomlinson, Jason M.; Toto, Tami

    2012-06-30

    A first-of-a-kind, extended-term cloud aircraft campaign was conducted to obtain an in-situ statistical characterization of boundary-layer clouds needed to investigate cloud processes and refine retrieval algorithms. Coordinated by the Atmospheric Radiation Measurement (ARM) Aerial Facility (AAF), the Routine AAF Clouds with Low Optical Water Depths (CLOWD) Optical Radiative Observations (RACORO) field campaign operated over the ARM Southern Great Plains (SGP) site from 22 January to 30 June 2009, collecting 260 h of data during 59 research flights. A comprehensive payload aboard the Center for Interdisciplinary Remotely-Piloted Aircraft Studies (CIRPAS) Twin Otter aircraft measured cloud microphysics, solar and thermal radiation, aerosol properties, and atmospheric state parameters. Proximity to the SGP's extensive complement of surface measurements provides ancillary data that supports modeling studies and enables evaluating a variety of surface retrieval algorithms. The five-month duration enabled sampling a range of conditions associated with the seasonal transition from winter to summer. Although about two-thirds of the cloud flights occurred in May and June, boundary-layer cloud fields were sampled under a variety of environmental and aerosol conditions, with about 75% of the flights occurring in cumulus and stratocumulus. Preliminary analyses show how these data are being used to analyze cloud-aerosol relationships, determine the aerosol sizes that are responsible for nucleating cloud drops, characterize the horizontal variability of the cloud radiative impacts, and evaluate air-borne and surface-based cloud property retrievals. We discuss how conducting an extended-term campaign requires a simplified operating paradigm that is different from that used for typical, short-term, intensive aircraft field programs.

  12. Effect of Boschniakia rossica on expression of GST-P, p53 and p21(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats.

    Science.gov (United States)

    Yin, Zong-Zhu; Jin, Hai-Ling; Yin, Xue-Zhe; Li, Tian-Zhu; Quan, Ji-Shu; Jin, Zeng-Nan

    2000-12-01

    AIM:To investigate the effect of Boschniakia rossica (BR) extract on expression of GST-P, p53 and p21(ras) proteins in early stage of chemical hepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS:The expression of tumor marker-placental form glutathione S-transferase (GST-P), p53 and p21(ras) proteins were investigated by immunohisto-chemical techniques and ABC method. Anti-inflammatory activities of BR were studied by xylene and croton oil-induced mouse ear edema, carrageenin, histamine and hot scald-induced rat pow edema, adjuvant-induced rat arthritis and cotton pellet induced mouse granuloma formation methods.RESULTS:The 500mg/kg of BR-H2O extract frac-tionated from BR-Methanol extract had inhibitory effect on the formation of DEN-induced GST-P-positive foci in rat liver (GST-P staining was 78% positive in DEN+AAF group vs 20% positive in DEN+AAF+BR group, Panti-inflamatory effect in xylene and crotonoil induced mouse ear edema (inhibitory rates were 26%-29% and 35%-59%, respectively). BR H(2)O extract exhibited inhibitory effect in carrageenin, histamine and hot scald-induced hind paw edema and adjuvant-induced arthritis in rats and cotton pellet-induced granuloma formation in mice.CONCLUSION:BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis.Both CH(2)Cl(2) and H(2)O extracts from BR exerted anti-inflammatory effect in rats and mice.

  13. Importance of quantification of local site effects based on wave propagation in seismic microzonation

    Indian Academy of Sciences (India)

    S Kumar; J P Narayan

    2008-11-01

    This paper presents the three most important aspects of seismic microzonation namely prediction of fundamental frequency (F0) of soil deposit, aggravation factor (aggravation factor is simply the extra spectral amplification due to complex 2D site effects over the 1D response of the soil column) and the spatial variability of the ground motion caused by the basin-edge induced Love waves. The predicted F0 of single, double and three-soil-layered models revealed that the available empirical relations to predict the F0 of layered soil deposits are inadequate. We recommend the use of analytical or numerical methods to predict such an important parameter based on wave propagation effects. An increase of amplitude of Love wave, strain level and average aggravation factor (AAF) with increase of impedance contrast was obtained. Based on the trend of rate of decrease of AAF and maximum strain with offset from the basin-edge, we can qualitatively infer that the effects of induced Love wave may reduce to a negligible value after a traveled distance of 6.5–10.0 (where is the wavelength corresponding to the 0 of soil layer). The obtained increase of strain level with the decrease of distance between two receiver points used for the computation of strain reflects that structures having spatial extent smaller than the may suffer damage due to the basin-edge induced surface waves. The fast rate of decrease of strain with the offset from the strong lateral discontinuity (SLD)/basin-edge may be attributed to the dispersive nature of Love wave. We can incorporate the increased spectral amplification due to the induced surface waves in the form of aggravation factor but till date we have no effective way to incorporate the effects of developed strain by induced surface waves in seismic microzonation or in building codes.

  14. Possibility of analytical finding of glycerol caused by self-catheterization in doping control.

    Science.gov (United States)

    Okano, Masato; Nishitani, Yasunori; Kageyama, Shinji

    2014-01-01

    Glycerol is listed on the World Anti-Doping Agency (WADA) prohibited list as a masking agent principally because the administration of glycerol increases plasma volume and decreases the concentration of haemoglobin and the value of haematocrit in blood. Glycerol is a naturally occurring substance; therefore, the threshold is set as 1.0 mg/mL in the WADA technical document (WADA TD2013DL). In a WADA-accredited doping control laboratory, three doping control urine specimens collected from impaired athletes were determined to contain a high concentration of glycerol (>1.0 mg/mL); two of these specimens were considered adverse analytical findings (AAFs). Self-catheterization is necessary for athletes with neurological disorders such as neurogenic bladder dysfunction. We conducted a simple simulation of self-catheterization as an experimental test using urethral catheters with an antiseptic agent containing glycerol to confirm the influence of this antiseptic agent on the quantitative value of glycerol in doping control analysis. Some users employ a catheter with glycerol solution (ca. 1 mL) to avoid pain during use. The urine sample passed through such a catheter exhibited a glycerol concentration (4.94 mg/mL) greater than the threshold level. In September 2014, the threshold for glycerol will change from 1.0 to 4.3 mg/mL (WADA TD2014DL); however, a possibility exists for the quantitative value of glycerol in doping control analysis to exceed the threshold because of the use of an antiseptic agent containing glycerol for self-catheterization. The AAF for glycerol for impaired athletes, particularly those who participate in Paralympic sports, should account for the use of a catheter with glycerol.

  15. Effect of Oenanthe Javanica Extract on Antioxidant Enzyme in the Rat Liver

    Directory of Open Access Journals (Sweden)

    Choong-Hyun Lee

    2015-01-01

    Full Text Available Background: Oenanthe javanica (O. javanica has been known to have high antioxidant properties via scavenging reactive oxygen species. We examined the effect of O. javanica extract (OJE on antioxidant enzymes in the rat liver. Methods: We examined the effect of the OJE on copper, zinc-superoxide dismutase (SOD1, manganese superoxide dismutase (SOD2, catalase (CAT, and glutathione peroxidase (GPx in the rat liver using immunohistochemistry and western blot analysis. Sprague-Dawley rats were randomly assigned to three groups; (1 normal diet fed group (normal-group, (2 diet containing ascorbic acid (AA-fed group (AA-group as a positive control, (3 diet containing OJE-fed group (OJE-group. Results: In this study, no histopathological finding in the rat liver was found in all the experimental groups. Numbers of SOD1, SOD2, CAT, and GPx immunoreactive cells and their protein levels were significantly increased in the AA-fed group compared with those in the normal-group. On the other hand, in the OJE-group, numbers of SOD1, SOD2, CAT, and GPx immunoreactive cells in the liver were significantly increased by about 190%, 478%, 685%, and 346%, respectively, compared with those in the AA-group. In addition, protein levels of SOD1, SOD2, CAT, and GPx in the OJE-group were also significantly much higher than those in the AA-group. Conclusion: OJE significantly increased expressions of SOD1 and SOD2, CAT, and GPx in the liver cells of the rat, and these suggests that significant enhancements of endogenous enzymatic antioxidants by OJE might be a legitimate strategy for decreasing oxidative stresses in the liver.

  16. Knowledge about Sounds – Context-Specific Meaning Differently Activates Cortical Hemispheres, Auditory Cortical Fields and Layers in House Mice

    Directory of Open Access Journals (Sweden)

    Diana B. Geissler

    2016-03-01

    Full Text Available Activation of the auditory cortex (AC by a given sound pattern is plastic, depending, in largely unknown ways, on the physiological state and the behavioral context of the receiving animal and on the receiver's experience with the sounds. Such plasticity can be inferred when house mouse mothers respond maternally to pup ultrasounds right after parturition and naïve females have to learn to respond. Here we use c-FOS immunocytochemistry to quantify highly activated neurons in the AC fields and layers of seven groups of mothers and naïve females who have different knowledge about and are differently motivated to respond to acoustic models of pup ultrasounds of different behavioral significance. Profiles of FOS-positive cells in the AC primary fields (AI, AAF, the ultrasonic field (UF, the secondary field (AII, and the dorsoposterior field (DP suggest that activation reflects in AI, AAF, and UF the integration of sound properties with animal state-dependent factors, in the higher-order field AII the news value of a given sound in the behavioral context, and in the higher-order field DP the level of maternal motivation and, by left-hemisphere activation advantage, the recognition of the meaning of sounds in the given context. Anesthesia reduced activation in all fields, especially in cortical layers 2/3. Thus, plasticity in the AC is field-specific preparing different output of AC fields in the process of perception, recognition and responding to communication sounds. Further, the activation profiles of the auditory cortical fields suggest the differentiation between brains hormonally primed to know (mothers and brains which acquired knowledge via implicit learning (naïve females. In this way, auditory cortical activation discriminates between instinctive (mothers and learned (naïve females cognition.

  17. Altered expression of cytochrome P450 and possible correlation with preneoplastic changes in early stage of rat hepatocarcinogenesis

    Institute of Scientific and Technical Information of China (English)

    Lin-lin LIU; Li-kun GONG; Xin-ming QI; Yan CAI; Hui WANG; Xiong-fei WU; Ying XIAO; Jin REN

    2005-01-01

    Aim: Correlation of cytochrome P450 (CYPs) with preneoplastic changes in the early stage of hepatocarcinogenesis is still unclear. To detect the expression of carcinogen-metabolizing related microsomal P450 enzymes, namely the CYP1A1,CYP1A2, CYP2B 1/2, CYP2E1, and CYP3A, we performed the medium-term bioassay of Ito's model in Sprague-Dawley rats. Methods: The amount and activity of CYP were assessed by biochemical and immunohistochemical methods in week 8.The correlation between CYP expression and microsomal oxidative stress was investigated by comparing the generation of microsomal lipid peroxidation in the presence or absence of specific CYP inhibitor. Results: In the DEN-2-AAF and 2-AAF alone groups, the expression of CYP1A1 and CYP2E1 were up-regulated and the expression of CYP2B 1/2 and CYP1A2 were quite the contrary. Strong staining of CYP2E1 and CYP2B1/2 was found around the centrolobular vein and weak staining in the altered hepatic foci revealed by immunohistochemical procedure.There was no significant change in the activity of CYP3A among the 4 groups.Altered hepatic tissue bore more microsomal NADPH (nicotinamide adenine dimucleotide phosphate,reduced form)-dependent lipid peroxidation than normal tissue. And the difference among the 4 groups disappeared when CYP2E1 was inhibited. More microsomal lipid peroxidation was generated when incubated with CYP1A inhibitor α-naphthoflavone. Conclusion: CYP altered their expression levels and these alterations can play important roles in the alteration of cell redox status of preneoplastic tissue in the early stage of hepatocarcinogenesis.

  18. Allergic proctocolitis refractory to maternal hypoallergenic diet in exclusively breast-fed infants: a clinical observation

    Directory of Open Access Journals (Sweden)

    Frediani Simone

    2011-07-01

    Full Text Available Abstract Background Allergic proctocolitis (APC in exclusively breast-fed infants is caused by food proteins, deriving from maternal diet, transferred through lactation. In most cases a maternal cow milk-free diet leads to a prompt resolution of rectal bleeding, while in some patients a multiple food allergy can occur. The aim of this study was to assess whether the atopy patch test (APT could be helpful to identify this subgroup of patients requiring to discontinue breast-feeding due to polisensitization. Additionally, we assessed the efficacy of an amino acid-based formula (AAF when multiple food allergy is suspected. amino acid-based formula Methods We have prospectively enrolled 14 exclusively breast-fed infants with APC refractory to maternal allergen avoidance. The diagnosis was confirmed by endoscopy with biopsies. Skin prick tests and serum specific IgE for common foods, together with APTs for common foods plus breast milk, were performed. After a 1 month therapy of an AAF all patients underwent a follow-up rectosigmoidoscopy. Results Prick tests and serum specific IgE were negative. APTs were positive in 100% infants, with a multiple positivity in 50%. Sensitization was found for breast milk in 100%, cow's milk (50%, soy (28%, egg (21%, rice (14%, wheat (7%. Follow-up rectosigmoidoscopy confirmed the remission of APC in all infants. Conclusions These data suggest that APT might become a useful tool to identify subgroups of infants with multiple gastrointestinal food allergy involving a delayed immunogenic mechanism, with the aim to avoid unnecessary maternal dietary restrictions before discontinuing breast-feeding.

  19. PREPARATION AND EVALUATION OF VITAMIN E ADJUVANTED OIL EMULSIFIED INFECTIOUS BRONCHITIS EXPERIMENTAL VACCINE

    Directory of Open Access Journals (Sweden)

    S. ALI, M. ARSHAD, M. SIDDIQUE AND M. ASHRAF

    2007-10-01

    Full Text Available The present study was conducted to prepare oil emulsified (OE infectious bronchitis (IB experimental vaccines. The vaccines were prepared using the vaccinal strain H-120 Infectious Bonchitis virus (IBV. The virus was cultivated in 9-day old embryonated eggs via allantoic cavity route. Allantoic-amniotic fluid (AAF was collected and inactivated with formalin @ 0.12%. Water in oil emulsion was prepared by adding one part of AAF to four parts of mineral oil containing water phase (Tween 80 and oil phase (Span 80 surfactants. Hydrophile lypohile balance (HLB of the emulsion was maintained at 7.0. Two oil emulsified experimental vaccines were prepared. Vaccine-I was prepared without vitamin E and Vaccine-II with vitamin E (300 mg/ml. A total of 120 day-old broiler breeder chickens were divided into 4 groups, A, B, C, and D, each having 30 birds. At the age of 21 days, experimental Vaccine-I, experimental vaccine-II and commercial IB killed (H-120 vaccine were inoculated @ 0.5 ml in the birds of groups A, B and C, respectively. Group D was maintained as nonvaccinated control. Efficacy of the vaccines was evaluated on the basis of humoral immune response (haemagglutination inhibition antibody titres against IB in the four groups. The seven weeks cumulative mean antibody titres (CMT of each group were calculated. The highest CMT was observed in group B (130, followed by group C (69, group A (58 and group D (17. Statistical analysis showed that haemagglutination inhibition (HI antibody titres in group B (vaccine- II were significantly higher than those of groups A, B and C (P< 0.05.

  20. Microfinance, wage employment and housework: a gender analysis.

    Science.gov (United States)

    Khan, M R

    1999-08-01

    This paper examines the loan-use pattern of women involved in wage employment and their benefits from such loans in Bangladesh. The effects of wage employment on gender relations and how these women balance loan use, wage employment, and housework were also explored. The study was conducted among women enrolled in the Ayesha Abed Foundation (AAF) of the Bangladesh Rural Advancement Committee (BRAC) in Jamalpur district, central Bangladesh. The AAF was established to generate employment and income for poor rural women. Data were gathered through survey, interviews, and focus-group discussions. Findings revealed that women wage earners avail themselves of the BRAC loans for consumption, asset accumulation, land purchase, and other productive purposes. About 53% of their loans were used by others and only 34 out of 341 women in the sample actually used the loans themselves. The loans were repaid by sewing or subsistence work, mainly in the subcenters (52%), through income from rickshaw pulling by their husbands (24%), and by selling vegetables, eggs, or milk. Furthermore, findings showed that the household work of women wage earners is generally taken up by other women in the family and has resulted in more men taking part in household responsibilities. In conclusion, wage employment plays an important factor in the promotion of the economic and social empowerment of women. Economic empowerment is observed in the greater degree of control women have over the money they earn. Social empowerment is manifested in the expanding mobility of women, whereby they are able to interact with other women and generate support systems.

  1. Effect of Oenanthe Javanica Extract on Antioxidant Enzyme in the Rat Liver

    Institute of Scientific and Technical Information of China (English)

    Choong-Hyun Lee; Joon-Ha Park; Jeong-Hwi Cho; In-Hye Kim; Ji-Hyeon Ahn; Jae-Chul Lee; Bai Hui Chen

    2015-01-01

    Background:Oenanthe javanica (O.javanica) has been known to have high antioxidant properties via scavenging reactive oxygen species.We examined the effect of O.javanica extract (OJE) on antioxidant enzymes in the rat liver.Methods:We examined the effect of the OJE on copper,zinc-superoxide dismutase (SOD1),manganese superoxide dismutase (SOD2),catalase (CAT),and glutathione peroxidase (GPx) in the rat liver using immunohistochemistry and western blot analysis.Sprague-Dawley rats were randomly assigned to three groups;(1) normal diet fed group (normal-group),(2) diet containing ascorbic acid (AA)-fed group (AA-group) as a positive control,(3) diet containing OJE-fed group (OJE-group).Results:In this study,no histopathological finding in the rat liver was found in all the experimental groups.Numbers of SOD1,SOD2,CAT,and GPx immunoreactive cells and their protein levels were significantly increased in the AA-fed group compared with those in the normal-group.On the other hand,in the OJE-group,numbers of SOD1,SOD2,CAT,and GPx immunoreactive cells in the liver were significantly increased by about 190%,478%,685%,and 346%,respectively,compared with those in the AA-group.In addition,protein levels of SOD 1,SOD2,CAT,and GPx in the OJE-group were also significantly much higher than those in the AA-group.Conclusion:OJE significantly increased expressions of SOD 1 and SOD2,CAT,and GPx in the liver cells of the rat,and these suggests that significant enhancements of endogenous enzymatic antioxidants by OJE might be a legitimate strategy for decreasing oxidative stresses in the liver.

  2. Knowledge About Sounds-Context-Specific Meaning Differently Activates Cortical Hemispheres, Auditory Cortical Fields, and Layers in House Mice.

    Science.gov (United States)

    Geissler, Diana B; Schmidt, H Sabine; Ehret, Günter

    2016-01-01

    Activation of the auditory cortex (AC) by a given sound pattern is plastic, depending, in largely unknown ways, on the physiological state and the behavioral context of the receiving animal and on the receiver's experience with the sounds. Such plasticity can be inferred when house mouse mothers respond maternally to pup ultrasounds right after parturition and naïve females have to learn to respond. Here we use c-FOS immunocytochemistry to quantify highly activated neurons in the AC fields and layers of seven groups of mothers and naïve females who have different knowledge about and are differently motivated to respond to acoustic models of pup ultrasounds of different behavioral significance. Profiles of FOS-positive cells in the AC primary fields (AI, AAF), the ultrasonic field (UF), the secondary field (AII), and the dorsoposterior field (DP) suggest that activation reflects in AI, AAF, and UF the integration of sound properties with animal state-dependent factors, in the higher-order field AII the news value of a given sound in the behavioral context, and in the higher-order field DP the level of maternal motivation and, by left-hemisphere activation advantage, the recognition of the meaning of sounds in the given context. Anesthesia reduced activation in all fields, especially in cortical layers 2/3. Thus, plasticity in the AC is field-specific preparing different output of AC fields in the process of perception, recognition and responding to communication sounds. Further, the activation profiles of the auditory cortical fields suggest the differentiation between brains hormonally primed to know (mothers) and brains which acquired knowledge via implicit learning (naïve females). In this way, auditory cortical activation discriminates between instinctive (mothers) and learned (naïve females) cognition.

  3. Pharmaceuticals and iodinated contrast media in a hospital wastewater: A case study to analyse their presence and characterise their environmental risk and hazard.

    Science.gov (United States)

    Mendoza, A; Aceña, J; Pérez, S; López de Alda, M; Barceló, D; Gil, A; Valcárcel, Y

    2015-07-01

    This work analyses the presence of twenty-five pharmaceutical compounds belonging to seven different therapeutic groups and one iodinated contrast media (ICM) in a Spanish medium-size hospital located in the Valencia Region. Analysis of the target compounds in the hospital wastewater was performed by means of solid phase extraction (SPE) followed by liquid chromatography-tandem mass spectrometry analysis (HPLC-MS/MS). A screening level risk assessment combining the measured environmental concentrations (MECs) with dose-response data based on Predicted No Effect Concentration (PNEC) was also applied to estimate Hazard Quotients (HQs) for the compounds investigated. Additionally, the environmental hazard associated to the various compounds measured was assessed through the calculation of the Persistence, Bioaccumulation and Toxicity (PBT) Index, which categorizes compounds according to their environmentally damaging characteristics. The results of the study showed the presence of twenty-four out of the twenty-six compounds analysed at individual concentrations ranging from 5 ng L(-1) to 2 mg L(-1). The highest concentrations corresponded to the ICM iomeprol, found at levels between 424 and 2093 μg L(-1), the analgesic acetaminophen (15-44 μg L(-1)), the diuretic (DIU) furosemide (6-15 μg L(-1)), and the antibiotics (ABIs) ofloxacin and trimethoprim (2-5 μg L(-1)). The lowest levels corresponded to the anti-inflammatory propyphenazone, found at concentrations between 5 and 44 ng L(-1). Differences in terms of concentrations of the analysed compounds have been observed in all the therapeutic groups when comparing the results obtained in this and other recent studies carried out in hospitals with different characteristics from different geographical areas and in different seasons. The screening level risk assessment performed in raw water from the hospital effluent showed that the analgesics and anti-inflammatories (AAFs) acetaminophen, diclofenac, ibuprofen and

  4. Relative cost-effectiveness of an extensively hydrolyzed casein formula containing the probiotic Lactobacillus rhamnosus GG in managing infants with cow's milk allergy in Italy

    Directory of Open Access Journals (Sweden)

    Guest JF

    2015-06-01

    Full Text Available Julian F Guest,1,2 Monica Panca,1 Olga Ovcinnikova,1 Rita Nocerino3 1CATALYST Health Economics Consultants, Northwood, Middlesex, UK; 2Faculty of Life Sciences and Medicine, King's College, London, UK; 3Department of Translational Medical Science, Pediatric Section, University of Naples 'Federico II', Naples, Italy Objective: To estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (eHCF containing the probiotic Lactobacillus rhamnosus GG, (eHCF + LGG; Nutramigen LGG as first-line management for cow's milk allergy (CMA compared with eHCF alone, soy-based formulae (SBF, hydrolyzed rice formulae (HRF, and amino acid formulae (AAF in Italy, from the perspective of the Italian National Health Service (INHS and parents. Methods: Decision modeling was used to estimate the probability of infants developing tolerance to cow's milk by 18 months, based on an observational study dataset. The model also estimated the cost (at 2012/2013 prices of health care resource use funded by the INHS and formulae paid for by parents over 18 months after starting a formula, as well as the relative cost-effectiveness of each of the formulae. Results: The probability of developing tolerance to cow's milk by 18 months was higher among infants with either IgE-mediated or non-IgE-mediated allergy who were fed eHCF + LGG compared to those fed one of the other formulae. The total health care cost of initially feeding infants with eHCF + LGG was less than that of feeding infants with one of the other formulae. Hence, eHCF + LGG affords the greatest value for money to both the INHS and parents of infants with either IgE-mediated or non-IgE-mediated CMA. Conclusion: Using eHCF + LGG instead of eHCF, SBF, HRF, or an AAF for first-line management of newly diagnosed infants with CMA in Italy affords a cost-effective use of publicly funded resources, and is cost-effective from the parents' perspective, since it improves outcome for less cost. A randomized

  5. Response to the society of toxicology task force re-examination of the ED01 study.

    Science.gov (United States)

    Kodell, R L; Gaylor, D W; Greenman, D L; Littlefield, N A; Farmer, J H

    1983-01-01

    This communication has re-examined and justified certain of the NCTR's analyses and recommendations from the ED01 Study, which were either misunderstood or misinterpreted by the SOT Task Force. In addition, we have shown that some of the Task Force's own analyses and interpretations are subject to review on scientific grounds. The Task Force's rejection of the linear extrapolation method recommended by the NCTR was stated because of a suspected force-fitting of a linear model to data, an approach that is not part of the NCTR procedure. In suspecting a protective effect of 2-AAF against bladder tumors, the Task Force used an inappropriate model that overpredicted the background bladder tumor rate in control mice. Contrary to the Task Force's belief, a failure to account adequately for time to tumor response was more characteristic of analyses performed by the Task Force rather than those performed by the NCTR. The Task Force's questioning of the multistage model for risk assessment was based on its use of inappropriate, crude tumor data rather than upon NCTR's use of the multistage model with time-adjusted tumor data. The Hartley-Sielken model did not fit the ED01 tumor data as well as the Task Force had presumed. In a risk extrapolation comparison by the Task Force, a coarse time partition of the ED01 data that had been questioned by the Task Force actually produced more stable results than a finer partition proposed by the Task Force. Another problem in the Task Force report concerns the change of protocol. Instead of resulting in a loss of strength as anticipated by the Task Force, the change of protocol during the ED01 Study resulted in an increase in information as alluded to by the Task Force. If the Task Force's proposal for restricting the length of feeding studies had been followed in the ED01 Study, most of the dose related tumor information would not have been obtained. Also, the Task Force's belief that low doses of 2-AAF had some effect on the

  6. The role of SDF-1/CXCR4 interaction and electron microscopic study of hepatic oval cells in rat%大鼠肝卵圆细胞的电镜观察及SDF-1/CXCR4轴作用研究

    Institute of Scientific and Technical Information of China (English)

    曾三平; 焦兴元; 黄晓明; 胡以则; 杜军

    2007-01-01

    目的 本研究通过应用AMD3100封闭卵圆细胞表面CXCR4受体,从而起到抑制趋化因子SDF-1的生物活性,观察大鼠肝卵圆细胞的生长及SDF-1,CXCR4 mRNA表达情况,探讨SDF-1/CXCR4轴在肝卵圆细胞激活、增殖、分化中所起的作用.方法 建立卵圆细胞增殖模型,分为四组,分别为:2-AAF/PH组,AMD3100/PH组,2-AAF/AMD3100/PH组,PH组,重(150±20)g的Wistar大鼠予2-AAF灌喂,联合2/3肝切除,制作卵圆细胞模型,并通过尾静脉注射AMD3100阻断SDF-1的生物学作用,分别在术后的第3、5、7、10、14、21天6个时间点各处死6只大鼠,取肝脏标本行CXCR4和SDF-1 RT-PCR,检测卵圆细胞CXCR4及SDF-1 mRNA的表达情况,并取第10天肝脏透射电镜检查,观察卵圆细胞的超微结构.结果 给予AMD3100抑制SDF-1作用后,肝脏卵圆细胞数量减少,细胞膜受体CXCR4表达下调,同时SDF-1表达也呈下调趋势.结论 抑制SDF-1活性可以在一定程度上减少卵圆细胞的增殖,SDF-1可以通过自分泌或旁分泌途径激活并促进肝卵圆细胞增殖.

  7. Establishment and evaluation of a model of short-term carcinogenesis for C57BL/6 mice%小鼠原发性肝癌短期诱导模型的建立和评价

    Institute of Scientific and Technical Information of China (English)

    刘勇; 赛岩; 刘冬梅; 刘杨; 文川; 范礼斌; 崔春萍

    2011-01-01

    Objective To establish a primary hepatoma model of mice in one month. Methods Physical injury and toxicity were induced in the liver of C57BL/6 mice through DEN intraperitoneal injection, liver regeneration was provoked with 30% partial hepatectomy (PH) ,2AAF was used to inhibit cell division of hepatic parenchymal cells to make sure that DEN toxicity would directly affect the liver oval cells to induce the mutation of the liver oval cells. Results 8(8/12) mice survived in the experimental group. The survival of each group accessed by RIA, IHC and WB indicated that almost each parameter or index was obviously changed. Conclusion According to the parameters appraised,the model of short-term carcinogenesis for C57BL/6 has been established successfully.%目的 用一个月的时间建立小鼠原发性肝癌模型.方法 用二乙基亚硝胺(DEN)通过腹腔注射的方式对C57BL/6小鼠进行肝脏物理损伤和毒性诱导,再经过30%部分肝切除(PH)刺激肝再生,同时辅以二乙酰基芴(2-AAF)抑制肝实质细胞分裂,使DEN的毒性诱导作用直接作用于肝脏卵圆细胞(OVC),诱导突变.结果 模型组存活8只(8/12),将存活的各组小鼠通过放射免疫分析(RIA)、免疫组化(IHC)和免疫印迹(WB)方法鉴定短期诱导小鼠原发性肝癌建模是否成功,结果发现原发性肝癌的绝大多数指标明显改变.结论 鉴定结果表明采用化学损伤结合部分肝切除方法,经过一个月时间成功诱导并建立了C57BL/6小鼠的原发性肝癌模型.

  8. Diagnosis and treatment progress of children′s food allergy correlative persistent diarrhea%儿童食物过敏相关性迁延慢性腹泻诊治进展

    Institute of Scientific and Technical Information of China (English)

    谢晓丽

    2015-01-01

    Food allergy ( FA ) often leads to protracted diarrhea and chronic diarrhea, which influence children′s nutrition and growth seriously and early diagnosis and treatment is particularly important. Its pathogenesis includes IgE mediated,not IgE mediated,and mix. Mainly manifestations include:food protein mediated rectum colitis, food proteins mediated enterocolitis syndrome,food proteins mediated bowel disease,and coeliac disease ( CD) . Diagnosis should be asked for details of the history. Diet avoidance and open food challenge tests help determine the diagnosis. Treatment mainly for suspicious foods to avoid,breastfeeding should be continue,mothers need to avoid food allergies, artificial feeding should be changed to extensively hydrolysed protein formula( eHF) or amino acid-based formula( AAF) feeding. Try not to use of antibiotics,and to give support therapy. Early diagnosis and treatment,correct nutritional gui-dance can prevent serious complications,reduce its incidence.%食物过敏( FA)常导致迁延慢性腹泻,严重影响儿童营养及生长发育,早期诊断和治疗尤其重要。其发病机制分为IgE介导、非IgE介导及IgE和非IgE共同介导。 FA相关性迁延慢性腹泻主要表现为食物蛋白性直肠结肠炎、食物蛋白性小肠结肠炎综合征、食物蛋白性肠病、麦胶样肠病( CD)。诊断需详细询问病史,饮食回避和开放性食物激发试验有助于明确诊断。治疗主要是针对可疑食物进行回避,母乳喂养儿继续母乳喂养,母亲需回避过敏食物,人工喂养儿需改为深度水解蛋白配方奶粉( eHF)或氨基酸配方奶粉( AAF)喂养。尽量不使用抗生素,并给予对症支持治疗。早期诊断和治疗、正确的营养指导可防止严重的并发症,降低其再发生率。

  9. Hepatic non-parenchymal cells and extracellular matrix participate in oval cell-mediated liver regeneration

    Institute of Scientific and Technical Information of China (English)

    Wei Zhang; Xiao-Ping Chen; Wan-Guang Zhang; Feng Zhang; Shuai Xiang; Han-Hua Dong; Lei Zhang

    2009-01-01

    AIM: To elucidate the interaction between nonparenchymal cells, extracellular matrix and oval cells during the restituting process of liver injury induced by partial hepatectomy (PH). METHODS: We examined the localization of oval cells, non-parenchymal cells, and the extracellular matrix components using immunohistochemical and double immunofluorescent analysis during the proliferation and differentiation of oval cells in N-2-acetylaminofluorene (2-AAF)/PH rat model. RESULTS: By day 2 after PH, small oval cells began to proliferate around the portal area. Most of stellate cells and laminin were present along the hepatic sinusoids in the periportal area. Kupffer cells and fibronectin markedly increased in the whole hepatic lobule. From day 4 to 9, oval cells spread further into hepatic parenchyma, closely associated with stellate cells, fibronectin and laminin. Kupffer cells admixed with oval cells by day 6 and then decreased in the periportal zone. From day 12 to 15, most of hepatic stellate cells (HSCs), laminin and fibronectin located around the small hepatocyte nodus, and minority of them appeared in the nodus. Kupffer cells were mainly limited in the pericentral sinusoids. After day 18, the normal liver lobule structures began to recover.CONCLUSION: Local hepatic microenvironment may participate in the oval cell-mediated liver regeneration through the cell-cell and cell-matrix interactions.

  10. Biomass Burning Observation Project (BBOP) Final Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Kleinman, LI [Brookhaven National Lab. (BNL), Upton, NY (United States); Sedlacek, A. J. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2016-01-01

    The Biomass Burning Observation Project (BBOP) was conducted to obtain a better understanding of how aerosols generated from biomass fires affect the atmosphere and climate. It is estimated that 40% of carbonaceous aerosol produced originates from biomass burning—enough to affect regional and global climate. Several biomass-burning studies have focused on tropical climates; however, few campaigns have been conducted within the United States, where millions of acres are burned each year, trending to higher values and greater climate impacts because of droughts in the West. Using the Atmospheric Radiation Measurement (ARM) Aerial Facility (AAF), the BBOP deployed the Gulfstream-1 (G-1) aircraft over smoke plumes from active wildfire and agricultural burns to help identify the impact of these events and how impacts evolve with time. BBOP was one of very few studies that targeted the near-field time evolution of aerosols and aimed to obtain a process-level understanding of the large changes that occur within a few hours of atmospheric processing.

  11. Antimutagenic properties of Mangifera indica L. stem bark extract and evaluation of its effects on hepatic CYP1A1.

    Science.gov (United States)

    Morffi, Janet; Rodeiro, Idania; Hernández, Sandra Luz; González, Leonora; Herrera, Jose; Espinosa-Aguirre, J Javier

    2012-09-01

    Mangifera indica stem bark extract (MSBE) is a Cuban natural product which has shown strong antioxidant properties. In this work, the antimutagenic effect of MSBE was tested against 10 well-known mutagens/carcinogens in the Ames test in the absence or presence of metabolic fraction (S9). The chemical mutagens tested included: cyclophosphamide, mitomycin C, bleomycin, cisplatin, dimethylnitrosamine (DMNA), benzo[a]pyrene (BP), 2-acetylaminofluorene (2-AAF), sodium azide, 1-nitropyrene (1-NP) and picrolonic acid. Protective effects of the extract were also evaluated by comparing the efficiency of S9 fraction obtained from rats treated during 28 days with oral doses of MSBE (50-500 mg/kg) with that obtained from rats treated with vehicle (control) to activate bleomycin and cyclophosphamide in the Ames test. MSBE concentrations between 50 and 500 μg/plate significantly reduced the mutagenicity mediated by all the chemicals tested with the exception of sodium azide. Higher mutagenicity was found when bleomycin and cyclophosphamide (CP) were activated by control S9 than by MSBE S9. In addition, inhibition of CYP1A1 microsomal activity was observed in the presence of MSBE (10-20 μg/ml). We can conclude that besides its potent antioxidant activity previously reported, MSBE may also exert a chemoprotective effect due to its capacity to inhibit CYP activity.

  12. Dissociation Dynamics of XPC-RAD23B from Damaged DNA Is a Determining Factor of NER Efficiency.

    Directory of Open Access Journals (Sweden)

    Benjamin Hilton

    Full Text Available XPC-RAD23B (XPC plays a critical role in human nucleotide excision repair (hNER as this complex recognizes DNA adducts to initiate NER. To determine the mutagenic potential of structurally different bulky DNA damages, various studies have been conducted to define the correlation of XPC-DNA damage equilibrium binding affinity with NER efficiency. However, little is known about the effects of XPC-DNA damage recognition kinetics on hNER. Although association of XPC is important, our current work shows that the XPC-DNA dissociation rate also plays a pivotal role in achieving NER efficiency. We characterized for the first time the binding of XPC to mono- versus di-AAF-modified sequences by using the real time monitoring surface plasmon resonance technique. Strikingly, the half-life (t1/2 or the retention time of XPC in association with damaged DNA shares an inverse relationship with NER efficiency. This is particularly true when XPC remained bound to clustered adducts for a much longer period of time as compared to mono-adducts. Our results suggest that XPC dissociation from the damage site could become a rate-limiting step in NER of certain types of DNA adducts, leading to repression of NER.

  13. Phototoxic and modulatory effects of natural products from the skin of Rhinella jimi (Stevaux, 2002

    Directory of Open Access Journals (Sweden)

    Samuel V. Brito

    2012-02-01

    Full Text Available The skin of amphibians possesses a large diversity of biologically active compounds that are associated with the natural defenses of these animals against pathogens. Five different extracts and fractions were obtained from the skin of Rhinella jimi: methanol extract (ME, methanol fractions (MF, chloroform extract of methanol extract (CF, aqueous alkaloid fraction (AAF and aqueous non-alkaloid fraction (ANAF. All fractions were evaluated with respect to their antibiotic modifying activity in standard bacterial strains and multiresistant clinical isolates. Antagonism was detected with kanamycin and gentamicin when combined with substances obtained from the skin of R. jimi. Phototoxic activity was observed in the methanol and chlorophorm fractions, as well as the aqueous non-alkaloid fraction. The antagonistic action was apparently associated with the protection afforded by the bacterial populations that inhabit the skin of this amphibian, preventing colonization by pathogenic fungi. The phototoxic activity demonstrated by natural products from the skin of R. jimi showed an interruption of the bacterial growth after UV exposure. This could indicate an antibacterial effect activated by the UV light, opening a path for carrying the attack by pathogenic fungi, causing the disease related with the amphibian decline.

  14. Adherence and virulence genes of Escherichia colifrom children diarrhoea in the Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Najla Benevides-Matos

    2015-03-01

    Full Text Available The bacterial pathogen most commonly associated with endemic forms of childhood diarrhoea is Escherichia coli. Studies of epidemiological characteristics of HEp-2 cell-adherent E. coli in diarrhoeal disease are required, particularly in developing countries. The aim of this study was evaluate the presence and significance of adherent Escherichia coli from diarrhoeal disease in children. The prevalence of LA, AA, and DA adherence patterns were determined in HEp-2 cells, the presence of virulence genes and the presence of the O serogroups in samples obtained from 470 children with acute diarrhoea and 407 controls in Porto Velho, Rondônia, Brazil. E. coli isolates were identified by PCR specific for groups of adherent E. coli. Out of 1,156 isolates obtained, 128 (11.0% were positive for eae genes corresponding to EPEC, however only 38 (29.6% of these amplified bfpAgene. EAEC were isolated from 164 (14.1% samples; of those 41(25%, 32 (19% and 16 (9.7% amplified eagg, aggA or aafA genes, respectively and aggA was significantly associated with diarrhoea (P = 0.00006. DAEC identified by their adhesion pattern and there were few isolates. In conclusion, EAEC was the main cause of diarrhoea in children, especially when the aggA gene was present, followed by EPEC and with a negligible presence of DAEC.

  15. Adherence and virulence genes of Escherichia coli from children diarrhoea in the Brazilian Amazon.

    Science.gov (United States)

    Benevides-Matos, Najla; Pieri, Fabio A; Penatti, Marilene; Orlandi, Patrícia P

    2015-03-01

    The bacterial pathogen most commonly associated with endemic forms of childhood diarrhoea is Escherichia coli . Studies of epidemiological characteristics of HEp-2 cell-adherent E. coli in diarrhoeal disease are required, particularly in developing countries. The aim of this study was evaluate the presence and significance of adherent Escherichia coli from diarrhoeal disease in children. The prevalence of LA, AA, and DA adherence patterns were determined in HEp-2 cells, the presence of virulence genes and the presence of the O serogroups in samples obtained from 470 children with acute diarrhoea and 407 controls in Porto Velho, Rondônia, Brazil. E. coli isolates were identified by PCR specific for groups of adherent E. coli . Out of 1,156 isolates obtained, 128 (11.0%) were positive for eae genes corresponding to EPEC, however only 38 (29.6%) of these amplified bfpA gene . EAEC were isolated from 164 (14.1%) samples; of those 41(25%), 32 (19%) and 16 (9.7%) amplified eagg , aggA or aafA genes, respectively and aggA was significantly associated with diarrhoea ( P = 0.00006). DAEC identified by their adhesion pattern and there were few isolates. In conclusion, EAEC was the main cause of diarrhoea in children, especially when the aggA gene was present, followed by EPEC and with a negligible presence of DAEC.

  16. Oenanthe javanica extract increases immunoreactivities of antioxidant enzymes in the rat kidney

    Institute of Scientific and Technical Information of China (English)

    Hyun-Jin Tae; Joon Ha Park; Jeong-Hwi Cho; In Hye Kim; Ji Hyeon Ahn; Jae Chul Lee; Jong-Dai Kim

    2014-01-01

    Background Oenanthe javanica is an aquatic perennial herb originated from East Asia.Nowadays,the effects of Oenanthe javanica have been proven in various disease models.Studies regarding the antioxidant effect of Oenanthe javanica in the kidney are still unclear.Methods This study was therefore performed to investigate the effect of the Oenanthe javanica extract (OJE) in the rat kidney using immunohistochemistry for antioxidant enzymes,copper,zinc-superoxide dismutase (SOD1),manganese superoxide dismutase (SOD2),catalase (CAT) and glutathione peroxidase (GPx).Sprague-Dawley rats were randomly assigned to three groups:(1) normal diet fed-group (normal-group),(2) diet containing ascorbic acid (AA)-fed group (AA-group) as a positive control,(3) diet containing OJE-fed group (OJE-group).AA and OJE were supplied during 28 days.Results The side-effects were not observed in all the groups.Immunoreactivities of SOD1,SOD2,CAT and GPx were easily detected in the distal tubules of the kidney,and their immunoreactivities in the AA-and OJE-groups were increased to about 1.4-1.5 times and 2 times,respectively,compared with those in the normal-group.Conclusion OJE significantly increased expressions of SOD1 & 2,CAT and GPx immunoreactivities in the distal tubules of the rat kidney,and this finding suggests that significant enhancements of endogenous enzymatic antioxidants by OJE treatment may be a legitimate strategy for decreasing oxidative stresses in the kidney.

  17. Requirement of Rad5 for DNA Polymerase ζ-Dependent Translesion Synthesis in Saccharomyces cerevisiae

    Science.gov (United States)

    Pagès, Vincent; Bresson, Anne; Acharya, Narottam; Prakash, Satya; Fuchs, Robert P.; Prakash, Louise

    2008-01-01

    In yeast, Rad6–Rad18-dependent lesion bypass involves translesion synthesis (TLS) by DNA polymerases η or ζ or Rad5-dependent postreplication repair (PRR) in which error-free replication through the DNA lesion occurs by template switching. Rad5 functions in PRR via its two distinct activities—a ubiquitin ligase that promotes Mms2–Ubc13-mediated K63-linked polyubiquitination of PCNA at its lysine 164 residue and a DNA helicase that is specialized for replication fork regression. Both these activities are important for Rad5's ability to function in PRR. Here we provide evidence for the requirement of Rad5 in TLS mediated by Polζ. Using duplex plasmids carrying different site-specific DNA lesions—an abasic site, a cis–syn TT dimer, a (6-4) TT photoproduct, or a G-AAF adduct—we show that Rad5 is needed for Polζ-dependent TLS. Rad5 action in this role is likely to be structural, since neither the inactivation of its ubiquitin ligase activity nor the inactivation of its helicase activity impairs its role in TLS. PMID:18757916

  18. Differential Modification of Cortical and Thalamic Projections to Cat Primary Auditory Cortex Following Early- and Late-Onset Deafness.

    Science.gov (United States)

    Chabot, Nicole; Butler, Blake E; Lomber, Stephen G

    2015-10-15

    Following sensory deprivation, primary somatosensory and visual cortices undergo crossmodal plasticity, which subserves the remaining modalities. However, controversy remains regarding the neuroplastic potential of primary auditory cortex (A1). To examine this, we identified cortical and thalamic projections to A1 in hearing cats and those with early- and late-onset deafness. Following early deafness, inputs from second auditory cortex (A2) are amplified, whereas the number originating in the dorsal zone (DZ) decreases. In addition, inputs from the dorsal medial geniculate nucleus (dMGN) increase, whereas those from the ventral division (vMGN) are reduced. In late-deaf cats, projections from the anterior auditory field (AAF) are amplified, whereas those from the DZ decrease. Additionally, in a subset of early- and late-deaf cats, area 17 and the lateral posterior nucleus (LP) of the visual thalamus project concurrently to A1. These results demonstrate that patterns of projections to A1 are modified following deafness, with statistically significant changes occurring within the auditory thalamus and some cortical areas. Moreover, we provide anatomical evidence for small-scale crossmodal changes in projections to A1 that differ between early- and late-onset deaf animals, suggesting that potential crossmodal activation of primary auditory cortex differs depending on the age of deafness onset.

  19. Integration of XRootD into the cloud infrastructure for ALICE data analysis

    CERN Document Server

    Kompaniets, Mikhail; Svirin, Pavlo; Yurchenko, Volodymyr; Zarochentsev, Andrey

    2015-01-01

    Cloud technologies allow easy load balancing between different tasks and projects. From the viewpoint of the data analysis in the ALICE experiment, cloud allows to deploy software using Cern Virtual Machine (CernVM) and CernVM File System (CVMFS), to run different (including outdated) versions of software for long term data preservation and to dynamically allocate resources for different computing activities, e.g. grid site, ALICE Analysis Facility (AAF) and possible usage for local projects or other LHC experiments.We present a cloud solution for Tier-3 sites based on OpenStack and Ceph distributed storage with an integrated XRootD based storage element (SE). One of the key features of the solution is based on idea that Ceph has been used as a backend for Cinder Block Storage service for OpenStack, and in the same time as a storage backend for XRootD, with redundancy and availability of data preserved by Ceph settings. For faster and easier OpenStack deployment was applied the Packstack solution, which is ba...

  20. Relationships of Entrainment Rate with Dynamical and Thermodynamic Properties in Shallow Convection

    Science.gov (United States)

    Lu, C.; Liu, Y.; Zhang, G. J.; Wu, X.; Endo, S.; Cao, L.; Li, Y.; Guo, X.

    2015-12-01

    This work examines the relationships of entrainment rate to vertical velocity, buoyancy, turbulent dissipation rate by applying stepwise principal component regression to observational data from shallow cumulus clouds collected during the Routine AAF [Atmospheric Radiation Measurement (ARM) Aerial Facility] Clouds with Low Optical Water Depths (CLOWD) Optical Radiative Observations (RACORO) field campaign over the ARM Southern Great Plains (SGP) site near Lamont, Oklahoma. The cumulus clouds during the RACORO campaign simulated using a large eddy simulation (LES) model are also examined with the same approach. The analysis shows that combination of multiple variables can better represent entrainment rate in both the observations and LES than the single-variable fitting equations and the three commonly used parameterizations. A new parameterization is thus presented that relates entrainment rate to vertical velocity, buoyancy and dissipation rate; the effects of treating clouds as ensembles and humid shells surrounding cumulus clouds on the new parameterization are discussed. Physical mechanisms underlying the relationships of entrainment rate to vertical velocity, buoyancy and dissipation rate are also explored. Furthermore, the effects of relative humidity in the entrained dry air on the above relationships are discussed; a possible physical mechanism for the effects is explored.

  1. Interaction of enteroaggregative Escherichia coli with salad leaves.

    Science.gov (United States)

    Berger, Cedric N; Shaw, Robert K; Ruiz-Perez, Fernando; Nataro, James P; Henderson, Ian R; Pallen, Mark J; Frankel, Gad

    2009-08-01

    Enteroaggregative Escherichia coli (EAEC) are important human pathogens. However, their environmental reservoir is unknown. As fresh salad leaves are increasingly recognized as an important environmental vector for human pathogens, we investigated leaf attachment capability of EAEC strains. We found that binding of clinical EAEC isolates to leaves from Eruca vesicaria (commonly known as rocket or arugula) can be divided into high, moderate and low adherent phenotypes. Using the prototype EAEC strain 042 to investigate the underlining mechanisms involved in leaf attachment, we found small attached bacterial aggregates over the entire leaf surface and dense bacterial attachment to the guard cell of the stomata. An aaf 042 mutant lost the ability to bind the epidermis while retaining stomatal adherence. In contrast, a fliC 042 mutant retained the ability to bind the epidermis but lost stomatal tropism. These results show that multiple adherence factors are involved in the interaction of EAEC with leaves, that EAEC uses similar colonization factors to bind mucosal and leaf surfaces and that fresh produce might be an important reservoir of EAEC strains.

  2. Summary of Meteorological Observations, Surface (SMOS), New River, North Carolina

    Science.gov (United States)

    1988-12-01

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  3. Expression characteristics and specific antibody reactivity of diverse cathepsin F members of Paragonimus westermani.

    Science.gov (United States)

    Ahn, Chun-Seob; Na, Byoung-Kuk; Chung, Dong-Ll; Kim, Jeong-Geun; Kim, Jin-Taek; Kong, Yoon

    2015-02-01

    Paragonimiasis, caused by the lung fluke Paragonimus, is a major food-borne helminthic disease. Differential diagnosis of paragonimiasis from tuberculosis and other infectious granulomas in the lung is a prerequisite to proper management of patients. Cysteine proteases of Paragonimus westermani (PwCPs) invoke specific antibody responses against patient sera, while antibody capturing activity of different PwCPs has not been comparatively analyzed. In this study, we observed the expressional regulation of 11 species of different PwCPs (PwCP1-11). We expressed recombinant PwCPs and assessed diagnostic reliability employing sera from patients with P. westermani (n=138), other trematodiases (n=80), cestodiases (n=60) and pulmonary tuberculosis (n=20), and those of normal controls (n=20). PwCPs formed a monophyletic clade into cathepsin F and showed differential expression patterns along with developmental stages of worm. Bacterially expressed recombinant PwCPs (rPwCPs) exhibited variable sensitivity of 38.4-84.5% and specificity of 87.2-100% in diagnosing homologous infection. rPwCPs recognized specific antibodies of experimental cat sera as early as 3 or 6weeks after infection. Patient sera of fascioliasis, Schistosomiasis japonicum and clonorchiasis demonstrated weak cross-reactions. Our results demonstrate that diverse PwCPs of the cathepsin F family participate in inducing specific antibody responses. Most P. westermani cathepsin F, except for PwCP2 (AAF21461), which showed negligible antibody responses, might be applicable for paragonimiasis serodiagnosis.

  4. Metagenomics reveals flavour metabolic network of cereal vinegar microbiota.

    Science.gov (United States)

    Wu, Lin-Huan; Lu, Zhen-Ming; Zhang, Xiao-Juan; Wang, Zong-Min; Yu, Yong-Jian; Shi, Jin-Song; Xu, Zheng-Hong

    2017-04-01

    Multispecies microbial community formed through centuries of repeated batch acetic acid fermentation (AAF) is crucial for the flavour quality of traditional vinegar produced from cereals. However, the metabolism to generate and/or formulate the essential flavours by the multispecies microbial community is hardly understood. Here we used metagenomic approach to clarify in situ metabolic network of key microbes responsible for flavour synthesis of a typical cereal vinegar, Zhenjiang aromatic vinegar, produced by solid-state fermentation. First, we identified 3 organic acids, 7 amino acids, and 20 volatiles as dominant vinegar metabolites. Second, we revealed taxonomic and functional composition of the microbiota by metagenomic shotgun sequencing. A total of 86 201 predicted protein-coding genes from 35 phyla (951 genera) were involved in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of Metabolism (42.3%), Genetic Information Processing (28.3%), and Environmental Information Processing (10.1%). Furthermore, a metabolic network for substrate breakdown and dominant flavour formation in vinegar microbiota was constructed, and microbial distribution discrepancy in different metabolic pathways was charted. This study helps elucidating different metabolic roles of microbes during flavour formation in vinegar microbiota.

  5. Auditory cortical areas activated by slow frequency-modulated sounds in mice.

    Directory of Open Access Journals (Sweden)

    Yuusuke Honma

    Full Text Available Species-specific vocalizations in mice have frequency-modulated (FM components slower than the lower limit of FM direction selectivity in the core region of the mouse auditory cortex. To identify cortical areas selective to slow frequency modulation, we investigated tonal responses in the mouse auditory cortex using transcranial flavoprotein fluorescence imaging. For differentiating responses to frequency modulation from those to stimuli at constant frequencies, we focused on transient fluorescence changes after direction reversal of temporally repeated and superimposed FM sweeps. We found that the ultrasonic field (UF in the belt cortical region selectively responded to the direction reversal. The dorsoposterior field (DP also responded weakly to the reversal. Regarding the responses in UF, no apparent tonotopic map was found, and the right UF responses were significantly larger in amplitude than the left UF responses. The half-max latency in responses to FM sweeps was shorter in UF compared with that in the primary auditory cortex (A1 or anterior auditory field (AAF. Tracer injection experiments in the functionally identified UF and DP confirmed that these two areas receive afferent inputs from the dorsal part of the medial geniculate nucleus (MG. Calcium imaging of UF neurons stained with fura-2 were performed using a two-photon microscope, and the presence of UF neurons that were selective to both direction and direction reversal of slow frequency modulation was demonstrated. These results strongly suggest a role for UF, and possibly DP, as cortical areas specialized for processing slow frequency modulation in mice.

  6. Research and application of the visualization system for the initial allocation of water rights%初始水权分配可视化系统研究与应用

    Institute of Scientific and Technical Information of China (English)

    黄俊铭; 解建仓; 阮宏勋; 张建龙; 王伟

    2009-01-01

    To use the MVC design pattern, AAF framework technology, knowledge map and components reuse techniques,researched the visualization system for the distribution of water rights. Taken the economic development and water resources in Wudinghe River Basin as the research object, combined with information technology, from the initial allocation of water rights needs, the paper formulated the visualization application system for the initial allocation of water rights. The results shown that the system can be quickly carried out the initial water right allocation and dynamical adjustment and provide a convenient, fast distribution system of water rights for the management department.%采用MVC设计模式、AAF框架技术、知识图以及组件复用技术研究水权分配的可视化应用系统.以无定河流域的经济发展和水资源概况为研究对象,结合信息化技术,从初始水权分配需求出发,制订流域初始水权分配的可视化应用系统.结果表明该系统的建立能够快速地对初始水权进行分配和动态调整,可为管理部门提供一种方便、快捷的水权分配系统.

  7. Integration of XRootD into the cloud infrastructure for ALICE data analysis

    Science.gov (United States)

    Kompaniets, Mikhail; Shadura, Oksana; Svirin, Pavlo; Yurchenko, Volodymyr; Zarochentsev, Andrey

    2015-12-01

    Cloud technologies allow easy load balancing between different tasks and projects. From the viewpoint of the data analysis in the ALICE experiment, cloud allows to deploy software using Cern Virtual Machine (CernVM) and CernVM File System (CVMFS), to run different (including outdated) versions of software for long term data preservation and to dynamically allocate resources for different computing activities, e.g. grid site, ALICE Analysis Facility (AAF) and possible usage for local projects or other LHC experiments. We present a cloud solution for Tier-3 sites based on OpenStack and Ceph distributed storage with an integrated XRootD based storage element (SE). One of the key features of the solution is based on idea that Ceph has been used as a backend for Cinder Block Storage service for OpenStack, and in the same time as a storage backend for XRootD, with redundancy and availability of data preserved by Ceph settings. For faster and easier OpenStack deployment was applied the Packstack solution, which is based on the Puppet configuration management system. Ceph installation and configuration operations are structured and converted to Puppet manifests describing node configurations and integrated into Packstack. This solution can be easily deployed, maintained and used even in small groups with limited computing resources and small organizations, which usually have lack of IT support. The proposed infrastructure has been tested on two different clouds (SPbSU & BITP) and integrates successfully with the ALICE data analysis model.

  8. Development of a Medium-term Animal Model Using gpt Delta Rats to Evaluate Chemical Carcinogenicity and Genotoxicity

    Science.gov (United States)

    Matsushita, Kohei; Kijima, Aki; Ishii, Yuji; Takasu, Shinji; Jin, Meilan; Kuroda, Ken; Kawaguchi, Hiroaki; Miyoshi, Noriaki; Nohmi, Takehiko; Ogawa, Kumiko; Umemura, Takashi

    2013-01-01

    In this study, the potential for development of an animal model (GPG46) capable of rapidly detecting chemical carcinogenicity and the underlying mechanisms of action were examined in gpt delta rats using a reporter gene assay to detect mutations and a medium-term rat liver bioassay to detect tumor promotion. The tentative protocol for the GPG46 model was developed based on the results of dose-response exposure to diethylnitrosamine (DEN) and treatment with phenobarbital over time following DEN administration. Briefly, gpt delta rats were exposed to various chemicals for 4 weeks, followed by a partial hepatectomy (PH) to collect samples for an in vivo mutation assay. The mutant frequencies (MFs) of the reporter genes were examined as an indication of tumor initiation. A single intraperitoneal (ip) injection of 10 mg/kg DEN was administered to rats 18 h after the PH to initiate hepatocytes. Tumor-promoting activity was evaluated based on the development of glutathione S-transferase placental form (GST-P)-positive foci at week 10. The genotoxic carcinogens 2-acetylaminofluorene (2-AAF), 2-amino-3-methylimidazo [4,5-f] quinolone (IQ) and safrole (SF), the non-genotoxic carcinogens piperonyl butoxide (PBO) and phenytoin (PHE), the non-carcinogen acetaminophen (APAP) and the genotoxic non-hepatocarcinogen aristolochic acid (AA) were tested to validate the GPG46 model. The validation results indicate that the GPG46 model could be a powerful tool in understanding chemical carcinogenesis and provide valuable information regarding human risk hazards. PMID:23723564

  9. Relationship of mouse body weight and food consumption/wastage to cage shelf level.

    Science.gov (United States)

    Greenman, D L; Bryant, P; Kodell, R L; Sheldon, W

    1983-12-01

    The relationships between the shelf level at which mice were housed and body weight and food consumption/wastage were examined by analyzing data obtained from the first 9 months of a carcinogenic dose-response study carried out in 24,192 female BALB/c mice using 2-acetylaminofluorene (2-AAF) as the carcinogen. Each cage, containing four mice, was maintained on a rack at a given shelf level throughout the course of the experiment to allow analysis of data by shelf level. The amount of food removed from each feeder and the body weight of each mouse was measured weekly. Average body weight gain during the first 12 weeks was lowest on the top shelf, highest on the next two shelves, and progressively lower as the shelf approached the lowest level on the rack. Body weights attained by week 36 of the experiment generally reflected this same pattern. Weekly food consumption/wastage, as measured by disappearance of food from the feeder, generally showed the converse shelf-level pattern as that shown by body weight gain. Removal of food from feeders was greatest on the top shelf, lowest on the next shelf, and progressively increased with each succeedingly lower shelf. Although it is not clear whether the food removal pattern was related more to food consumption or food wastage, the observation was reproducible.

  10. Joint Subcarrier Pairing and Resource Allocation for Cognitive Network and Adaptive Relaying Strategy

    KAUST Repository

    Soury, Hamza

    2012-05-01

    Recent measurements show that the spectrum is under-utilized by licensed users in wireless communication. Cognitive radio (CR) has been proposed as a suitable solution to manage the inefficient usage of the spectrum and increase coverage area of wireless networks. The concept is based on allowing a group of secondary users (SUs) to share the unused radio spectrum originally owned by the primary user (PUs). The operation of CR should not cause harmful interference to the PUs. In the other hand, relayed transmission increases the coverage and achievable capacity of communication systems and in particular in CR systems. In fact there are many types of cooperative communications, however the two main ones are decode-and-forward (DAF) and amplify-and-forward (AAF). Adaptive relaying scheme is a relaying technique by which the benefits of the amplifying or decode and forward techniques can be achieved by switching the forwarding technique according to the quality of the signal. In this dissertation, we investigate the power allocation for an adaptive relaying protocol (ARP) scheme in cognitive system by maximizing the end-to-end rate and searching the best carriers pairing distribution. The optimization problem is under the interference and power budget constraints. The simulation results confirm the efficiency of the proposed adaptive relaying protocol in comparison to other relaying techniques, and the consequence of the choice of the pairing strategy.

  11. STIL2 in India - Pilot study 2012

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-07-01

    Sweden and India have a bilateral agreement regarding energy issues and during the spring of 2011 a number of projects were launched by the Swedish Energy Agency. One of them was the implementation of STIL2 in India. The STIL2 in India report prepared by AaF consultant for the Swedish Energy Agency aims to launch a project 'Implementation of STIL2 in India' under BEE-STEM cooperation. BEE stands for the Indian Bureau of Energy Efficiency and STEM stands for the Swedish Energy Agency. The report presents the energy use in a specific building category on national level in India. The STIL2 project as such does not render any quantifiable energy savings, rather a baseline of the energy use with objective and harmonised data output. Reference values constitute a prerequisite for quantifying change over time and full scale STIL2 implementation provides a strong set of reference values. Since the data is presented on national level it allows to show estimated and calculated energy saving potentials for the whole building stock. These results can be used to support energy efficiency, monitor progress and as a base for policy development. This is the first project of several within the BEE-STEM cooperation.

  12. Adherence and virulence genes of Escherichia coli from children diarrhoea in the Brazilian Amazon

    Science.gov (United States)

    Benevides-Matos, Najla; Pieri, Fabio A.; Penatti, Marilene; Orlandi, Patrícia P.

    2015-01-01

    The bacterial pathogen most commonly associated with endemic forms of childhood diarrhoea is Escherichia coli . Studies of epidemiological characteristics of HEp-2 cell-adherent E. coli in diarrhoeal disease are required, particularly in developing countries. The aim of this study was evaluate the presence and significance of adherent Escherichia coli from diarrhoeal disease in children. The prevalence of LA, AA, and DA adherence patterns were determined in HEp-2 cells, the presence of virulence genes and the presence of the O serogroups in samples obtained from 470 children with acute diarrhoea and 407 controls in Porto Velho, Rondônia, Brazil. E. coli isolates were identified by PCR specific for groups of adherent E. coli . Out of 1,156 isolates obtained, 128 (11.0%) were positive for eae genes corresponding to EPEC, however only 38 (29.6%) of these amplified bfpA gene . EAEC were isolated from 164 (14.1%) samples; of those 41(25%), 32 (19%) and 16 (9.7%) amplified eagg , aggA or aafA genes, respectively and aggA was significantly associated with diarrhoea ( P = 0.00006). DAEC identified by their adhesion pattern and there were few isolates. In conclusion, EAEC was the main cause of diarrhoea in children, especially when the aggA gene was present, followed by EPEC and with a negligible presence of DAEC. PMID:26221098

  13. Joint sub-carrier pairing and resource allocation for cognitive networks with adaptive relaying

    KAUST Repository

    Soury, Hamza

    2013-11-09

    Relayed transmission in a cognitive radio (CR) environment could be used to increase the coverage and capacity of communication system that benefits already from the efficient management of the spectrum developed by CR. Furthermore, there are many types of cooperative communications, including decode-and-forward (DAF) and amplify-and-forward (AAF). In this paper, these techniques are combined in an adaptive mode to benefit from its forwarding advantages; this mode is called adaptive relaying protocol (ARP). Moreover, this work focuses on the joint power allocation in a cognitive radio system in a cooperative mode that operates ARP in multi-carrier mode. The multi-carrier scenario is used in an orthogonal frequency division multiplexing (OFDM) mode, and the problem is formulated to maximize the end-to-end rate by searching the best power allocation at the transmitters. This work includes, besides the ARP model, a sub-carrier pairing strategy that allows the relays to switch to the best sub-carrier pairs to increase the throughput. The optimization problem is formulated and solved under the interference and power budget constraints using the sub-gradient algorithm. The simulation results confirm the efficiency of the proposed adaptive relaying protocol in comparison to other relaying techniques. The results show also the consequence of the choice of the pairing strategy. 2013 Stolojescu-Crisan and Isar; licensee Springer.

  14. ARM-ACME V: ARM Airborne Carbon Measurements V on the North Slope of Alaska Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Biraud, Sebastien C [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2016-05-01

    Atmospheric temperatures are warming faster in the Arctic than predicted by climate models. The impact of this warming on permafrost degradation is not well understood, but it is projected to increase carbon decomposition and greenhouse gas production (CO2 and/or CH4) by arctic ecosystems. Airborne observations of atmospheric trace gases, aerosols and cloud properties in North Slopes of Alaska (NSA) are improving our understanding of global climate, with the goal of reducing the uncertainty in global and regional climate simulations and projections. From June 1 through September 15, 2015, AAF deployed the G1 research aircraft and flew over the North Slope of Alaska (38 flights, 140 science flight hours), with occasional vertical profiling over Prudhoe Bay, Oliktok point, Barrow, Atqasuk, Ivotuk, and Toolik Lake. The aircraft payload included Picarro and Los Gatos Research (LGR) analyzers for continuous measurements of CO2, CH4, H2O, and CO and N2O mixing ratios, and a 12-flask sampler for analysis of carbon cycle gases (CO2, CO, CH4, N2O, 13CO2, and trace hydrocarbon species). The aircraft payload also include measurements of aerosol properties (number size distribution, total number concentration, absorption, and scattering), cloud properties (droplet and ice size information), atmospheric thermodynamic state, and solar/infrared radiation.

  15. Survey of Forensic Document Examination Habit Areas: Degree of Use and Discriminatory Power

    Energy Technology Data Exchange (ETDEWEB)

    G Sperry; PA Manzolillo; RC Hanlan; RJ Muehlberger

    1999-09-07

    Beginning in 1998, the Pacific Northwest National Laboratory (PNL), US Postal Inspection Service Forensic Laboratory (USPIS), and the Data Fusion Laboratory, Drexel University (DFL) have been collaborating on a large scale research project ''Handwriting Individuality--Moving From Art to Science''. In April 1998 a survey was distributed to the community of forensic document examiners (FDEs) requesting input on the habit areas used and their utility in distinguishing handwriting. The information obtained from this survey was intended to provide the data necessary to select the criteria and begin the evaluation of the handwriting samples currently in the project. Preliminary results of the survey were made available to the community at the American Society of Questioned Document Examiners (ASQDE) meeting in August 1998 and the American Academy of Forensic Sciences (AAFS) meeting in February 1999. This report provides final documentation of the survey and its results. This survey has two objectives: (1) to compile a list of handwriting features and characteristics used by professional forensic document examiners in the examination and comparison of handwriting and (2) to gather information about the significance of these features and characteristics. These objectives are met by having the FDEs provide an indication of their experience in the frequency of habit area evaluation and the utility of the habit area for discrimination.

  16. 端粒酶在大鼠肝卵圆细胞中表达的意义%Expression and its significance of telomerase in rat hepatic oval cells

    Institute of Scientific and Technical Information of China (English)

    张伟; 陈孝平; 项帅; 张峰; 董汉华; 张磊; 陈琳; 张万广

    2009-01-01

    目的 检测大鼠肝卵圆细胞端粒酶活性,探讨端粒酶表达与卵圆细胞增殖分化的关系.方法 采用2-AAF/PH模型诱导大鼠卵圆细胞增殖,改良的胶原酶灌注结合密度梯度离心法分离,用细胞免疫荧光和电镜鉴定卵圆细胞.采用免疫组织化学、RT-PCR、荧光定量PCR等方法检测卵圆细胞端粒酶的表达,组间比较采用t检验分析其意义.结果 采用2-AAF/PH模型成功诱导大鼠卵圆细胞增殖.分离的卵圆细胞核大,卵圆形,胞质少,呈铺路石样生长.电镜发现细胞核质比大,胞质内细胞器少且发育不成熟.细胞免疫荧光结果表达OV-6、AFP、CK-19、albumin、c-kit.端粒酶逆转录酶(TERT)表达在门静脉周围增殖的卵圆细胞核内,随着卵圆细胞逐渐向肝细胞方向分化,TERT阳性细胞数逐渐减少.大鼠正常肝组织TERT mRNA表达水平最高,为LE-6卵圆细胞的2.27倍;分离的卵圆细胞TERT mRNA表达水平为LE.6卵圆细胞的1.26倍;采用2μg和4μg细胞提取物分析细胞的端粒酶活性,随着LE-6卵圆细胞传代次数的增加,由24代传至40代,端粒酶活性由△A=1.05、1.15降低到△A=0.25、0.45(t=17.74,12.38,P<0.05).结论 卵圆细胞具有端粒酶活性,端粒酶可能是卵圆细胞维持其增殖能力和多分化潜能的一个必要条件.%Objcctive To detect the telomerase activity in rat hepatic oval cells, and to explore the relationship between telomerase expression and the proliferation and differentiation of oval cells. Methods The 2-acetamidofluorene/partial bepatectomy (2-AAF/PH) rat model was used to induce the proliferation of oval cells. Oval cells were isolated by modified collagenase perfusion and gradient centrifugation. Electron microscope exami-nation and immunofluorescence were adopted to identify oval cells. Immunohistochemistry, RT-PCR and fluores-cence quantitative PCR were used to detect the expression of telomerase in oval cells. All the data collected were analyzed by

  17. Multiscale mapping of frequency sweep rate in mouse auditory cortex.

    Science.gov (United States)

    Issa, John B; Haeffele, Benjamin D; Young, Eric D; Yue, David T

    2017-02-01

    Functional organization is a key feature of the neocortex that often guides studies of sensory processing, development, and plasticity. Tonotopy, which arises from the transduction properties of the cochlea, is the most widely studied organizational feature in auditory cortex; however, in order to process complex sounds, cortical regions are likely specialized for higher order features. Here, motivated by the prevalence of frequency modulations in mouse ultrasonic vocalizations and aided by the use of a multiscale imaging approach, we uncover a functional organization across the extent of auditory cortex for the rate of frequency modulated (FM) sweeps. In particular, using two-photon Ca(2+) imaging of layer 2/3 neurons, we identify a tone-insensitive region at the border of AI and AAF. This central sweep region behaves fundamentally differently from nearby neurons in AI and AII, responding preferentially to fast FM sweeps but not to tones or bandlimited noise. Together these findings define a second dimension of organization in the mouse auditory cortex for sweep rate complementary to that of tone frequency.

  18. Reproductive Incompatibility Involving Senegalese Aedes aegypti (L) Is Associated with Chromosome Rearrangements

    Science.gov (United States)

    Dickson, Laura B.; Sharakhova, Maria V.; Timoshevskiy, Vladimir A.; Fleming, Karen L.; Caspary, Alex; Sylla, Massamba; Black, William C.

    2016-01-01

    Aedes aegypti, the primary vector of dengue, yellow fever and Zika flaviviruses, consists of at least two subspecies. Aedes aegypti (Aaa) is light in color, has pale scales on the first abdominal tergite, oviposits in artificial containers, and preferentially feeds on humans. Aedes aegypti formosus (Aaf), has a dark cuticle, is restricted to sub-Saharan Africa, has no pale scales on the first abdominal tergite and frequently oviposits in natural containers. Scale patterns correlate with cuticle color in East Africa but not in Senegal, West Africa where black cuticle mosquitoes display a continuum of scaling patterns and breed domestically indoors. An earlier laboratory study did not indicate any pre- or postzygotic barriers to gene flow between Aaa and Aaf in East Africa. However, similar attempts to construct F1 intercross families between Aaa laboratory strains and Senegal Ae. aegypti (SenAae) failed due to poor F1 oviposition and low F2 egg-to-adult survival. Insemination and assortative mating experiments failed to identify prezygotic mating barriers. Backcrosses were performed to test for postzygotic isolation patterns consistent with Haldane’s rule modified for species, like Aedes, that have an autosomal sex determining locus (SDL). Egg-pupal survival was predicted to be low in females mated to hybrid F1 males but average when a male mates with a hybrid F1 female. Survival was in fact significantly reduced when females mated to hybrid males but egg-pupal survival was significantly increased when males were mated to hybrid F1 females. These observations are therefore inconclusive with regards to Haldane’s rule. Basic cytogenetic analyses and Fluorescent In Situ Hybridization (FISH) experiments were performed to compare SenAae strains with the IB12 strain of Aaa that was used for genome sequencing and physical mapping. Some SenAae strains had longer chromosomes than IB12 and significantly different centromeric indices on chromosomes 1 and 3. DAPI staining

  19. Reproductive Incompatibility Involving Senegalese Aedes aegypti (L) Is Associated with Chromosome Rearrangements.

    Science.gov (United States)

    Dickson, Laura B; Sharakhova, Maria V; Timoshevskiy, Vladimir A; Fleming, Karen L; Caspary, Alex; Sylla, Massamba; Black, William C

    2016-04-01

    Aedes aegypti, the primary vector of dengue, yellow fever and Zika flaviviruses, consists of at least two subspecies. Aedes aegypti (Aaa) is light in color, has pale scales on the first abdominal tergite, oviposits in artificial containers, and preferentially feeds on humans. Aedes aegypti formosus (Aaf), has a dark cuticle, is restricted to sub-Saharan Africa, has no pale scales on the first abdominal tergite and frequently oviposits in natural containers. Scale patterns correlate with cuticle color in East Africa but not in Senegal, West Africa where black cuticle mosquitoes display a continuum of scaling patterns and breed domestically indoors. An earlier laboratory study did not indicate any pre- or postzygotic barriers to gene flow between Aaa and Aaf in East Africa. However, similar attempts to construct F1 intercross families between Aaa laboratory strains and Senegal Ae. aegypti (SenAae) failed due to poor F1 oviposition and low F2 egg-to-adult survival. Insemination and assortative mating experiments failed to identify prezygotic mating barriers. Backcrosses were performed to test for postzygotic isolation patterns consistent with Haldane's rule modified for species, like Aedes, that have an autosomal sex determining locus (SDL). Egg-pupal survival was predicted to be low in females mated to hybrid F1 males but average when a male mates with a hybrid F1 female. Survival was in fact significantly reduced when females mated to hybrid males but egg-pupal survival was significantly increased when males were mated to hybrid F1 females. These observations are therefore inconclusive with regards to Haldane's rule. Basic cytogenetic analyses and Fluorescent In Situ Hybridization (FISH) experiments were performed to compare SenAae strains with the IB12 strain of Aaa that was used for genome sequencing and physical mapping. Some SenAae strains had longer chromosomes than IB12 and significantly different centromeric indices on chromosomes 1 and 3. DAPI staining was

  20. Arctic Black Carbon Loading and Profile Using the Single-Particle Soot Photometer (SP2) Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Sedlacek, Arthur J [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2016-05-01

    s deployment within the Arctic Circle during the summer of 2015 (Deadhorse, Alaska [70° 12' 20" N, 148° 30' 42" W]), the truly unique opportunity presented itself to acquire profile data on BC loading at little additional cost. Since the SP2 is a particle-resolved measurement, the resulting data set provides refractory black carbon (rBC) mass loadings, size and mass distributions, and rBC-containing particle mixing state, all of which are expected to readily find value in the modeling community. As part of the ACME-V (http://www.arm.gov/campaigns/aaf2014armacmev) campaign, CO, CO2, and CH4 were also measured, providing the unique opportunity for carbon closure. We will also work closely with modelers who require such data and expect this collaboration will lead directly to a better understanding of the climate impacts of BC in the Arctic. The primary measurement objective was to acquire airborne data on the vertical and spatial distributions of refractory black carbon (rBC) loading, size and mass distribution, and particle mixing state. The primary scientific objective was to provide a targeted data set of rBC particle distributions to better understand and constrain the impact of black carbon radiative forcing in the cryosphere. The SP2-based data set during this campaign is available in the DOE-ARM archive (http://www.arm.gov/campaigns/aaf2015abclp).

  1. Metabolic activation of N-hydroxy arylamines, N-hydroxy heterocyclic amines and ring-hydroxymethyl polycyclic aromatic hydrocarbons by human sulfotransferases

    Energy Technology Data Exchange (ETDEWEB)

    Chou, H.C.

    1993-01-01

    Arylamines and polycyclic aromatic hydrocarbons (PAHs) are two major classes of chemical carcinogens. N-Hydroxylation of arylamines is regarded to be a necessary process for their mutagenicity and carcinogenicity, while alkyl-hydroxylation is the major metabolic pathway for alkyl-substituted PAHs. Evidence has been presented that sulfation of several N-hydroxy arylamines and hydroxymethyl PAHs is an important pathway leading to the formation of ultimate carcinogens in experiment animals. Sulfation of these chemicals forms putative sulfuric acid ester intermediates that can rearrange to electrophilic nitrenium or carbenium ions capable of forming covalent adducts with important cellular macromolecules. In order to study the metabolic activation by sulfotransferase(s) in various human tissue preparations an in vitro enzymatic assay was established. A metabolic phenotyping method was also developed for thermostable phenolsulfotransferase (TS-PST) in platelet homogenates (correlated with TS-PST activity in other tissues) based on a simple colorimetric assay using 2-naphthol as substrate. By using a PAPS-regenerating system to supply the activated sulfate and calf thymus DNA to trap the reactive metabolites, we found that N-hydroxy derivatives of the carcinogens, 4-aminobiphenyl (4-ABP), 4,4[prime]-methylene-bis(2-chloroaniline) (MOCA), 2-acetylaminofluorene (2-AAF), 2-aminofluorene (2-AF), 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP), and 2-amino-6-methyldipyrido [1,2-1:3[prime],2[prime]-d]imidazole (Glu-P-1) were metabolically activated by human TS-PST. On the other hand, three methyl-hydroxylated derivatives (7-OH, 12-OH, and 7,12-diOH) of 7, 12-dimethylbenz[a]anthracene (DMBA) were metabolically activated by human steroid sulfotransferase. Human sulfotransferase(s)-mediated activation of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) was not observed.

  2. Influence of serum from liver-damaged rats on differentiation tendency of bone marrow-derived stem cells

    Institute of Scientific and Technical Information of China (English)

    Hai Hong; Jian-Zhi Chen; Feng Zhou; Ling Xue; Guo-Qiang Zhao

    2004-01-01

    AIM: Recent studies in both rodents and humans indicated that bone marrow (BM)-derived stem cells were able to home to the liver after they were damaged and demonstrated plasticity in becoming hepatocytes. However, the question remains as to how these stem cells are activated and led to the liver and where the signals initiating the mechanisms of activation and differentiation of stem cells originate. The aim of this study was to investigate the influence of serum from liver-damaged rats on differentiation tendency of bone marrow-derived stem cells.METHODS: Serum samples were collected from rats treated with a 2-acetylaminofluorene (2-AAF)/carbon tetrachloride (CCl4) program for varying time points and then used as stimulators of cultured BM stem cells. Expression of M2- and L-type isozymes of rat pyruvate kinase, albumin as well as integrin-β1 were then examined by reverse transcription polymerase chain reaction (RT-PCR) to estimate the differentiation state of BM stem cells.RESULTS: Expression of M2-type isozyme of pyruvate kinase (M2-PK), a marker of immature hepatocytes, was detected in each group stimulated with experimental serum, but not in controls including mature hepatocytes, BM stem cells without serum stimulation, and BM stem cells stimulated with normal control serum. As a marker expressed in the development of liver, the expression signal of integrin-β1 was also detectable in each group stimulated with experimental serum. However, expression of L-type isozyme of pyruvate kinase (L-PK) and albumin, marker molecules of mature hepatocytes, was not detected in groups stimulated with experimental serum.CONCLUSION: Under the influence of serum from rats with liver failure, BM stem cells begin to differentiate along a direction to hepatocyte lineage and to possess some features of immature hepatocytes.

  3. Determining Best Estimates and Uncertainties in Cloud Microphysical Parameters from ARM Field Data: Implications for Models, Retrieval Schemes and Aerosol-Cloud-Radiation Interactions

    Energy Technology Data Exchange (ETDEWEB)

    McFarquhar, Greg [Univ. of Illinois, Urbana, IL (United States)

    2015-12-28

    We proposed to analyze in-situ cloud data collected during ARM/ASR field campaigns to create databases of cloud microphysical properties and their uncertainties as needed for the development of improved cloud parameterizations for models and remote sensing retrievals, and for evaluation of model simulations and retrievals. In particular, we proposed to analyze data collected over the Southern Great Plains (SGP) during the Mid-latitude Continental Convective Clouds Experiment (MC3E), the Storm Peak Laboratory Cloud Property Validation Experiment (STORMVEX), the Small Particles in Cirrus (SPARTICUS) Experiment and the Routine AAF Clouds with Low Optical Water Depths (CLOWD) Optical Radiative Observations (RACORO) field campaign, over the North Slope of Alaska during the Indirect and Semi-Direct Aerosol Campaign (ISDAC) and the Mixed-Phase Arctic Cloud Experiment (M-PACE), and over the Tropical Western Pacific (TWP) during The Tropical Warm Pool International Cloud Experiment (TWP-ICE), to meet the following 3 objectives; derive statistical databases of single ice particle properties (aspect ratio AR, dominant habit, mass, projected area) and distributions of ice crystals (size distributions SDs, mass-dimension m-D, area-dimension A-D relations, mass-weighted fall speeds, single-scattering properties, total concentrations N, ice mass contents IWC), complete with uncertainty estimates; assess processes by which aerosols modulate cloud properties in arctic stratus and mid-latitude cumuli, and quantify aerosol’s influence in context of varying meteorological and surface conditions; and determine how ice cloud microphysical, single-scattering and fall-out properties and contributions of small ice crystals to such properties vary according to location, environment, surface, meteorological and aerosol conditions, and develop parameterizations of such effects.In this report we describe the accomplishments that we made on all 3 research objectives.

  4. A participação dos ergonomistas nos projetos organizacionais

    Directory of Open Access Journals (Sweden)

    Marçal Jackson

    1999-12-01

    Full Text Available Neste texto, pretende-se apresentar, em resposta às condicionantes conceituais e metodológicas colocadas por Salerno, uma abordagem metodológica, a abordagem da atividade futura (AAF, que viabiliza a participação dos ergonomistas em projetos organizacionais. Nessa abordagem, que tem a Análise Ergonômica do Trabalho (AET como base, os ergonomistas não sustentam unicamente a posição clínica, característica da AET, mas assumem o papel de "atores do processo de projeto" a fim de cooperar com os diferentes atores da empresa: diretores, gerentes, engenheiros, trabalhadores. Todavia, a participação efetiva da Ergonomia depende de nova forma de conceber a organização, que deve ser abordada não somente através de sua dimensão estrutural, mas também através das "regulações sociais necessárias à manutenção e à construção da organização do trabalho".In response to Salerno's theoretical and methodological constraints to ergonomists participation in organisational design process, this paper proposes a new approach based in future work activity forecasting. Even if the Ergonomie Work Analysis (EWA is the underlying methodological tool, in this approach, ergonomists must assume a new role, as "actors of the design process", and co-operate with the others company actors: managers, engineers, foremen and workers. But to be more effective, ergonomie intervention needs a new framework of "organisation" issues. To be more effective, it will be argued that one must approach organisâtionnal design not only by its structural components but also by its inherent social interactions and adjustments.

  5. Evaluation of long-term surface-retrieved cloud droplet number concentration with in situ aircraft observations: ARM Cloud Droplet Number Concentration

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Kyo-Sun Sunny [Pacific Northwest National Laboratory, Richland Washington USA; Riihimaki, Laura [Pacific Northwest National Laboratory, Richland Washington USA; Comstock, Jennifer M. [Pacific Northwest National Laboratory, Richland Washington USA; Schmid, Beat [Pacific Northwest National Laboratory, Richland Washington USA; Sivaraman, Chitra [Pacific Northwest National Laboratory, Richland Washington USA; Shi, Yan [Pacific Northwest National Laboratory, Richland Washington USA; McFarquhar, Greg M. [Department of Atmospheric Sciences, University of Illinois at Urbana-Champaign, Urbana Illinois USA

    2016-03-06

    A new cloud-droplet number concentration (NDROP) value added product (VAP) has been produced at the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) site for the 13 years from January 1998 to January 2011. The retrieval is based on surface radiometer measurements of cloud optical depth from the multi-filter rotating shadow-band radiometer (MFRSR) and liquid water path from the microwave radiometer (MWR). It is only applicable for single-layered warm clouds. Validation with in situ aircraft measurements during the extended-term aircraft field campaign, Routine ARM Aerial Facility (AAF) CLOWD Optical Radiative Observations (RACORO), shows that the NDROP VAP robustly reproduces the primary mode of the in situ measured probability density function (PDF), but produces a too wide distribution, primarily caused by frequent high cloud-droplet number concentration. Our analysis shows that the error in the MWR retrievals at low liquid water paths is one possible reason for this deficiency. Modification through the diagnosed liquid water path from the coordinate solution improves not only the PDF of the NDROP VAP but also the relationship between the cloud-droplet number concentration and cloud-droplet effective radius. Consideration of entrainment effects rather than assuming an adiabatic cloud improves the values of the NDROP retrieval by reducing the magnitude of cloud-droplet number concentration. Aircraft measurements and retrieval comparisons suggest that retrieving the vertical distribution of cloud-droplet number concentration and effective radius is feasible with an improvement of the parameter representing the mixing effects between environment and clouds and with a better understanding of the effect of mixing degree on cloud properties.

  6. Radiative Energetics of Mineral Dust Aerosol over Zhangye China during the AMY 2008 Field Campaign

    Science.gov (United States)

    Hansell, R. A.; Tsay, S.; Ji, Q.; Hsu, C.; Bell, S.; Li, C.; Wang, C.

    2010-12-01

    In support of the DOE ARM program, NASA Goddard’s mobile ground-based laboratories (SMART-COMMIT) were deployed to Zhangye China (39.082°N; 100.276°E) from April-June 2008 as an ARM Ancillary Facility (AAF) to support the Asian Monsoon Year (AMY) field study. The primary objective at Zhangye, a semi-arid region located between the Taklimakan and Gobi deserts, was to both capture and characterize dust aerosol near the source region and to determine its direct aerosol radiative effects (DARE). To facilitate this study, the AAF’s Atmospheric Emitted Radiance Interferometer (AERI), a key instrument for spectrally characterizing the thermal IR, is employed to retrieve the daytime/nighttime dust IR aerosol optical thickness (AOT) for several notable dust events. Regional dust microphysical and mineralogy measurements are also used for developing a representative aerosol optical model of dust single-scattering properties. The retrieved AOT are then inputted into a 1-D radiative transfer model constrained by local measurements to evaluate dust’s local instantaneous shortwave (SW) and longwave (LW) DARE at the surface and top of the atmosphere (TOA) along with the heating rate profiles under cloud-free atmospheres. Comparisons of the SW DARE are made with a previous study over the same area using broadband data from thermal dome effect corrected pyranometers and the significance of the LW effects relative to the SW is examined. This study is part of an on-going effort to complete a global assessment of dust DARE for the major dust source regions of the world.

  7. Surface Measurements of dust/local aerosol properties over Northern China during 2008 China-US joined dust field campaign

    Science.gov (United States)

    Wang, X.; Huang, J.

    2009-12-01

    The objective of this study is to understand the detailed characteristics and underlying mechanisms of aerosol physical and optical parameters over China Loess Plateau and its potential impacts on the regional/global climate. In order to characterize the emission, transport, and removal of atmospheric pollutants emitted from East Asia, the 2008 China-US joined field campaign are conducted from late April to May 2008 focused specifically on the Asian direct measurements of dust and pollution transport, following the plume from the Northern China which from the Taklamakan desert and Gobi desert to the Eastern Pacific and into North America. Such measurements are crucial to understanding how the dust and the pollution plume (including black carbon) are modified as their age. Three sites involved this campaign, including one permanent site (Semi-Arid Climate & Environment Observatory of Lanzhou University (SACOL)) (located in Yuzhong, 35.95N/104.1E), one SACOL's Mobile Facility (SMF) (deployed in Jintai, 37.57N/104.23E) and the U.S. Department of Energy Atmospheric Radiation Measurements(ARM) Ancillary Facility (AAF mobile laboratories, SMART-COMMIT) (deployed in Zhangye, 39.08N/100.27E). Results indicate that the dust plumes are transported from the surface to a long distance from their sources have a significant influence on the air quality in the study area. The meteorological analysis indicates that these polluted layers are not from local sources during dust plume and this large-scale transport of dust and pollutants remains a major uncertainty in quantifying the global effect of emissions from Northern China.

  8. Holistic Interactions of Shallow Clouds, Aerosols, and Land-Ecosystems (HI-SCALE) Science Plan

    Energy Technology Data Exchange (ETDEWEB)

    Fast, JD [Pacific Northwest National Laboratory; Berg, LK [Pacific Northwest National Laboratory

    2015-12-01

    Cumulus convection is an important component in the atmospheric radiation budget and hydrologic cycle over the Southern Great Plains and over many regions of the world, particularly during the summertime growing season when intense turbulence induced by surface radiation couples the land surface to clouds. Current convective cloud parameterizations contain uncertainties resulting in part from insufficient coincident data that couples cloud macrophysical and microphysical properties to inhomogeneities in boundary layer and aerosol properties. The Holistic Interactions of Shallow Clouds, Aerosols, and Land-Ecosystems (HI-SCALE) campaign is designed to provide a detailed set of measurements that are needed to obtain a more complete understanding of the life cycle of shallow clouds by coupling cloud macrophysical and microphysical properties to land surface properties, ecosystems, and aerosols. HI-SCALE consists of 2, 4-week intensive observational periods, one in the spring and the other in the late summer, to take advantage of different stages and distribution of “greenness” for various types of vegetation in the vicinity of the Atmospheric Radiation and Measurement (ARM) Climate Research Facility’s Southern Great Plains (SGP) site as well as aerosol properties that vary during the growing season. Most of the proposed instrumentation will be deployed on the ARM Aerial Facility (AAF) Gulfstream 1 (G-1) aircraft, including those that measure atmospheric turbulence, cloud water content and drop size distributions, aerosol precursor gases, aerosol chemical composition and size distributions, and cloud condensation nuclei concentrations. Routine ARM aerosol measurements made at the surface will be supplemented with aerosol microphysical properties measurements. The G-1 aircraft will complete transects over the SGP Central Facility at multiple altitudes within the boundary layer, within clouds, and above clouds.

  9. Recurrence quantification analysis applied to spatiotemporal pattern analysis in high-density mapping of human atrial fibrillation.

    Science.gov (United States)

    Zeemering, Stef; Bonizzi, Pietro; Maesen, Bart; Peeters, Ralf; Schotten, Ulrich

    2015-01-01

    Spatiotemporal complexity of atrial fibrillation (AF) patterns is often quantified by annotated intracardiac contact mapping. We introduce a new approach that applies recurrence plot (RP) construction followed by recurrence quantification analysis (RQA) to epicardial atrial electrograms, recorded with a high-density grid of electrodes. In 32 patients with no history of AF (aAF, n=11), paroxysmal AF (PAF, n=12) and persistent AF (persAF, n=9), RPs were constructed using a phase space electrogram embedding dimension equal to the estimated AF cycle length. Spatial information was incorporated by 1) averaging the recurrence over all electrodes, and 2) by applying principal component analysis (PCA) to the matrix of embedded electrograms and selecting the first principal component as a representation of spatial diversity. Standard RQA parameters were computed on the constructed RPs and correlated to the number of fibrillation waves per AF cycle (NW). Averaged RP RQA parameters showed no correlation with NW. Correlations improved when applying PCA, with maximum correlation achieved between RP threshold and NW (RR1%, r=0.68, p <; 0.001) and RP determinism (DET, r=-0.64, p <; 0.001). All studied RQA parameters based on the PCA RP were able to discriminate between persAF and aAF/PAF (DET persAF 0.40 ± 0.11 vs. 0.59 ± 0.14/0.62 ± 0.16, p <; 0.01). RP construction and RQA combined with PCA provide a quick and reliable tool to visualize dynamical behaviour and to assess the complexity of contact mapping patterns in AF.

  10. Embryonic turkey liver: activities of biotransformation enzymes and activation of DNA-reactive carcinogens

    Energy Technology Data Exchange (ETDEWEB)

    Perrone, Carmen E.; Duan, Jian Dong; Jeffrey, Alan M.; Williams, Gary M. [New York Medical College, Department of Pathology, Valhalla (United States); Ahr, Hans-Juergen; Schmidt, Ulrich [Bayer AG, Institute of Toxicology, Wuppertal (Germany); Enzmann, Harald H. [Federal Institute for Drugs and Medical Devices, Bonn (Germany)

    2004-10-01

    Avian embryos are a potential alternative model for chemical toxicity and carcinogenicity research. Because the toxic and carcinogenic effects of some chemicals depend on bioactivation, activities of biotransformation enzymes and formation of DNA adducts in embryonic turkey liver were examined. Biochemical analyses of 22-day in ovoturkey liver post-mitochondrial fractions revealed activities of the biotransformation enzymes 7-ethoxycoumarin de-ethylase (ECOD), 7-ethoxyresorufin de-ethylase (EROD), aldrin epoxidase (ALD), epoxide hydrolase (EH), glutathione S-transferase (GST), and UDP-glucuronyltransferase (GLUT). Following the administration of phenobarbital (24 mg/egg) on day 21, enzyme activities of ECOD, EROD, ALD, EH and GLUT, but not of GST, were increased by two-fold or higher levels by day 22. In contrast, acute administration of 3-methylcholanthrene (5 mg/egg) induced only ECOD and EROD activities. Bioactivation of structurally diverse pro-carcinogens was also examined using {sup 32}P-postlabeling for DNA adducts. In ovoexposure of turkey embryos on day 20 of gestation to 2-acetylaminofluorene (AAF), 4,4'-methylenebis(2-chloroaniline) (MOCA), benzo[a]pyrene (BaP), and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) resulted in the formation of DNA adducts in livers collected by day 21. Some of the DNA adducts had {sup 32}P-postlabeling chromatographic migration patterns similar to DNA adducts found in livers from Fischer F344 rats exposed to the same pro-carcinogens. We conclude that 21-day embryonic turkey liver is capable of chemical biotransformation and activation of genotoxic carcinogens to form DNA adducts. Thus, turkey embryos could be utilized to investigate potential chemical toxicity and carcinogenicity. (orig.)

  11. Discovery of Point Mutations in the Voltage-Gated Sodium Channel from African Aedes aegypti Populations: Potential Phylogenetic Reasons for Gene Introgression.

    Directory of Open Access Journals (Sweden)

    Hitoshi Kawada

    2016-06-01

    Full Text Available Yellow fever is endemic in some countries in Africa, and Aedes aegpyti is one of the most important vectors implicated in the outbreak. The mapping of the nation-wide distribution and the detection of insecticide resistance of vector mosquitoes will provide the beneficial information for forecasting of dengue and yellow fever outbreaks and effective control measures.High resistance to DDT was observed in all mosquito colonies collected in Ghana. The resistance and the possible existence of resistance or tolerance to permethrin were suspected in some colonies. High frequencies of point mutations at the voltage-gated sodium channel (F1534C and one heterozygote of the other mutation (V1016I were detected, and this is the first detection on the African continent. The frequency of F1534C allele and the ratio of F1534C homozygotes in Ae. aegypti aegypti (Aaa were significantly higher than those in Ae. aegypti formosus (Aaf. We could detect the two types of introns between exon 20 and 21, and the F1534C mutations were strongly linked with one type of intron, which was commonly found in South East Asian and South and Central American countries, suggesting the possibility that this mutation was introduced from other continents or convergently selected after the introgression of Aaa genes from the above area.The worldwide eradication programs in 1940s and 1950s might have caused high selection pressure on the mosquito populations and expanded the distribution of insecticide-resistant Ae. aegypti populations. Selection of the F1534C point mutation could be hypothesized to have taken place during this period. The selection of the resistant population of Ae. aegypti with the point mutation of F1534C, and the worldwide transportation of vector mosquitoes in accordance with human activity such as trading of used tires, might result in the widespread distribution of F1534C point mutation in tropical countries.

  12. Areas of cat auditory cortex as defined by neurofilament proteins expressing SMI-32.

    Science.gov (United States)

    Mellott, Jeffrey G; Van der Gucht, Estel; Lee, Charles C; Carrasco, Andres; Winer, Jeffery A; Lomber, Stephen G

    2010-08-01

    The monoclonal antibody SMI-32 was used to characterize and distinguish individual areas of cat auditory cortex. SMI-32 labels non-phosphorylated epitopes on the high- and medium-molecular weight subunits of neurofilament proteins in cortical pyramidal cells and dendritic trees with the most robust immunoreactivity in layers III and V. Auditory areas with unique patterns of immunoreactivity included: primary auditory cortex (AI), second auditory cortex (AII), dorsal zone (DZ), posterior auditory field (PAF), ventral posterior auditory field (VPAF), ventral auditory field (VAF), temporal cortex (T), insular cortex (IN), anterior auditory field (AAF), and the auditory field of the anterior ectosylvian sulcus (fAES). Unique patterns of labeling intensity, soma shape, soma size, layers of immunoreactivity, laminar distribution of dendritic arbors, and labeled cell density were identified. Features that were consistent in all areas included: layers I and IV neurons are immunonegative; nearly all immunoreactive cells are pyramidal; and immunoreactive neurons are always present in layer V. To quantify the results, the numbers of labeled cells and dendrites, as well as cell diameter, were collected and used as tools for identifying and differentiating areas. Quantification of the labeling patterns also established profiles for ten auditory areas/layers and their degree of immunoreactivity. Areal borders delineated by SMI-32 were highly correlated with tonotopically-defined areal boundaries. Overall, SMI-32 immunoreactivity can delineate ten areas of cat auditory cortex and demarcate topographic borders. The ability to distinguish auditory areas with SMI-32 is valuable for the identification of auditory cerebral areas in electrophysiological, anatomical, and/or behavioral investigations.

  13. Avian influenza virus (H5N1; effects of physico-chemical factors on its survival

    Directory of Open Access Journals (Sweden)

    Hameed Sajid

    2009-03-01

    Full Text Available Abstract Present study was performed to determine the effects of physical and chemical agents on infective potential of highly pathogenic avian influenza (HPAI H5N1 (local strain virus recently isolated in Pakistan during 2006 outbreak. H5N1 virus having titer 108.3 ELD50/ml was mixed with sterilized peptone water to get final dilution of 4HA units and then exposed to physical (temperature, pH and ultraviolet light and chemical (formalin, phenol crystals, iodine crystals, CID 20, virkon®-S, zeptin 10%, KEPCIDE 300, KEPCIDE 400, lifebuoy, surf excel and caustic soda agents. Harvested amnio-allantoic fluid (AAF from embryonated chicken eggs inoculated with H5N1 treated virus (0.2 ml/egg was subjected to haemagglutination (HA and haemagglutination inhibition (HI tests. H5N1 virus lost infectivity after 30 min at 56°C, after 1 day at 28°C but remained viable for more than 100 days at 4°C. Acidic pH (1, 3 and basic pH (11, 13 were virucidal after 6 h contact time; however virus retained infectivity at pH 5 (18 h, 7 and 9 (more than 24 h. UV light was proved ineffectual in inactivating virus completely even after 60 min. Soap (lifebuoy®, detergent (surf excel® and alkali (caustic soda destroyed infectivity after 5 min at 0.1, 0.2 and 0.3% dilution. All commercially available disinfectants inactivated virus at recommended concentrations. Results of present study would be helpful in implementing bio-security measures at farms/hatcheries levels in the wake of avian influenza virus (AIV outbreak.

  14. 牛奶蛋白诱导的过敏性直肠结肠炎患儿喂养干预研究%Feeding intervention study on allergic proctocolitis in children induced by cow milk protein

    Institute of Scientific and Technical Information of China (English)

    赵红梅; 游洁玉; 刘莉; 张文婷; 唐娟; 欧阳红娟; 陈志勇

    2014-01-01

    induced by cow milk protein,and to observe the effects of the feeding strategies for them with different interventions in feeding,and then to identify the best time and the best way to modify feeding strategies according to different individual.Methods The effectiveness of different feeding interventions was retrospectively analyzed after 2 weeks in 171 cases with allergic proctocolitis induced by cow milk protein in Hunan Children's Hospital during the period of Jan.2012 to Jul.2013.Thirty-two cases received breast feeding (18.7%) and 139 cases received non-breast feeding (81.3%).Intervention methods:32cases with breast feeding received the same feeding method;among 139 babies with non-breast feeding group,36 were plus free amino acid (AAF) (21.1%),and 103 were plus the extensively hydrolyzed formula (eHF).The intervention formula changes were observed in 8,12 and 24 weeks,respectively,and nutritional risks in 0,3,6 months were assessed,respectively.Results There were 171 patients totally,male to female ratio was 1.5 to 1.0,and the average age was (4.1 ± 1.3) months.After 2 weeks,147 cases were notably effective,accounting for 86%,and in the breast feeding group 79.2% of the patients were notably effective,and there was no significance compared with the AAF group and eHF group (P > 0.05).The intervention formulation changes during the following 6 months were as follows:in 8 weeks,30 cases in the breast feeding group breastfeeding continued,2 cases turned to eHF feeding for the lack of breast milk,21 cases were fed with AAF (12.3%),85 cases with eHF (49.7%),and 35 cases with moderately hydrolyzed formula (20.5%) ;in 12 weeks:23 cases had breast feeding (13.5%),16 cases were fed with free amino acid formula powder (9.4%),56 cases with extensively hydrolyzed formula (32.7%),and 76 cases with moderately hydrolyzed formula (44.4%) ;in 24 weeks:21 cases (12.3%) had breast feeding,7 cases were fed with AAF(4.1%),13 cases with moderately hydrolyzed

  15. ACAPEX – Ship-Based Ice Nuclei Collections Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    DeMott, Paul J [Colorado State Univ., Fort Collins, CO (United States); Hill, Thomas CJ [Colorado State Univ., Fort Collins, CO (United States)

    2016-04-01

    Measurements were sought to evaluate a hypotheses that sea-spray-sourced ice-nucleating particles (INPs) are of biological origin and represent a distinctly different INP population in comparison to long-range-transported desert or urban and regional land-sourced INP, and that the layering of marine within other aerosol layers feeding orographic storms over the mountains of California and the Western United States thereby leads to common and quantifiable scenarios that influence precipitation over the region. Aerosol collections on the National Oceanic and Atmospheric Administration (NOAA) research vessel (RV) Ronald H. Brown, for subsequent processing of INP immersion freezing activation temperature spectra and composition analyses, added a valuable measurement component to the ARM Cloud Aerosol Precipitation Experiment (ACAPEX) and related CalWater2 (NOAA) studies for use in parameterizing and modeling the impacts of marine boundary layer and other aerosols on climate and radiation via aerosol indirect effects on mixed-phase clouds. Twenty-five nominally 24-hour collections were made and have been processed for immersion freezing INP number concentrations versus temperature in the mixed-phase cloud temperature regime from -10 to -27°C. The similarity of INP number concentrations compared to typical marine boundary layer values attributed to sea-spray aerosols was noted. Nevertheless, variability of INP concentrations of up to 50 times was noted at individual temperatures over the course of the study. A particular analysis possible with this data set is to examine INP budgets over oceans inside versus outside of atmospheric river conditions. These INP measurements supplemented multiple airborne INP measurements on the ARM Aerial Facility (AAF), and others on the ground during ACAPEX and CalWater2, to provide extensive spatial and temporal analyses of INP immersion freezing spectra during winter storm periods. Future analyses will use thermal sensitivity to

  16. Development of a Bi-Disciplinary Course in Forensic Science

    Directory of Open Access Journals (Sweden)

    Stacey L. Raimondi

    2013-08-01

    Full Text Available Forensic science programs and courses have traditionally been housed within chemistry departments at the college/university level, largely because the pioneers of the field were chemists who applied technology that was more chemical than biological in nature. However, with the development of such areas of study as DNA analysis, anatomical studies, and forensic entomology, it is becoming more and more important for forensic science students to have a strong biological background as well as a chemical background. Furthermore, while biology students are typically required to have extensive chemistry training as part of their major, the converse is not true for chemistry students. Therefore, it is possible that a student interested in forensic science could complete a major in chemistry and never have taken a biology class, leaving them woefully under-prepared for any type of masters program or career in forensic science immediately following graduation. Indeed, an examination of available positions in forensic science shows a large number of positions for DNA analysts for which the typical chemistry student would not be prepared without extensive biology training (http://www.aafs.org. Furthermore, positions for medical examiners or pathologists require extensive training in biology in addition to the continued medical training and residency programs. Therefore, it seems imperative that introductory forensic science courses adapt to these needs and be taught with a more bi-disciplinary approach in order to educate students on the whole field rather than one aspect. To that end, a new bi-disciplinary Forensic Science course was developed at Elmhurst College. This course was team-taught by a biology and a chemistry professor so that students would obtain a thorough understanding of the field and techniques used by both biologists and chemists. A description of this new version of a forensic science course follows, focusing on the addition of biology

  17. Typical enteroaggregative Escherichia coli is the most prevalent pathotype among E. coli strains causing diarrhea in Mongolian children.

    Science.gov (United States)

    Sarantuya, Jav; Nishi, Junichiro; Wakimoto, Naoko; Erdene, Shirchin; Nataro, James P; Sheikh, Jalaluddin; Iwashita, Mayumi; Manago, Kunihiro; Tokuda, Koichi; Yoshinaga, Masao; Miyata, Koichiro; Kawano, Yoshifumi

    2004-01-01

    Diarrhea remains one of the main sources of morbidity and mortality in the world, and a large proportion is caused by diarrheagenic Escherichia coli. In Mongolia, the epidemiology of diarrheagenic E. coli has not been well studied. A total of 238 E. coli strains from children with sporadic diarrhea and 278 E. coli strains from healthy children were examined by PCR for 10 virulence genes: enteropathogenic E. coli (EPEC) eae, tir, and bfpA; enterotoxigenic E. coli (ETEC) lt and st; enteroinvasive E. coli (EIEC) ipaH; enterohemorragic E. coli stx1 and stx2; and enteroaggregative E. coli (EAEC) aggR and astA. EAEC strains without AggR were identified by the HEp-2 cell adherence test. The detection of EAEC, ETEC, EPEC, and EIEC was significantly associated with diarrhea. The incidence of EAEC (15.1%), defined by either a molecular or a phenotypic assay, was higher in the diarrheal group than any other category (0 to 6.0%). The incidence of AggR-positive EAEC in the diarrheal group was significantly higher than in the control group (8.0 versus 1.4%; P = 0.0004), while that of AggR-negative EAEC was not (7.1 versus 4.3%). Nineteen AggR-positive EAEC strains harbored other EAEC virulence genes-aggA, 2 (5.5%); aafA, 4 (11.1%); agg-3a, 5 (13.8%); aap, 8 (22.2%); aatA, 11 (30.5%); capU, 9 (25.0%); pet, 6 (16.6%); and set, 3 (8.3%)-and showed 15 genotypes. EAEC may be an important pathogen of sporadic diarrhea in Mongolian children. Genetic analysis showed the heterogeneity of EAEC but illustrated the importance of the AggR regulon (denoting typical EAEC) as a marker for virulent EAEC strains.

  18. Zika Virus Is Not Uniquely Stable at Physiological Temperatures Compared to Other Flaviviruses

    Science.gov (United States)

    Goo, Leslie; Dowd, Kimberly A.; Smith, Alexander R. Y.; Pelc, Rebecca S.; DeMaso, Christina R.

    2016-01-01

    ABSTRACT Zika virus (ZIKV) is a flavivirus that has emerged as a global health threat due in part to its association with congenital abnormalities. Other globally relevant flaviviruses include dengue virus (DENV) and West Nile virus (WNV). High-resolution structures of ZIKV reveal many similarities to DENV and suggest some differences, including an extended glycan loop (D. Sirohi, Z. Chen, L. Sun, T. Klose, T. C. Pierson, et al., 352:467–470, 2016, http://dx.doi.org/10.1126/science.aaf5316) and unique interactions among envelope (E) protein residues that were proposed to confer increased virion stability and contribute mechanistically to the distinctive pathobiology of ZIKV (V. A. Kostyuchenko, E. X. Lim, S. Zhang, G. Fibriansah, T. S. Ng, et al., Nature 533:425–428, 2016, http://dx.doi.org/10.1038/nature17994). However, in the latter study, virus stability was inferred by measuring the loss of infectivity following a short incubation period. Here, we rigorously assessed the relative stability of ZIKV, DENV, and WNV by measuring changes in infectivity following prolonged incubation at physiological temperatures. At 37°C, the half-life of ZIKV was approximately twice as long as the half-life of DENV (11.8 and 5.2 h, respectively) but shorter than that of WNV (17.7 h). Incubation at 40°C accelerated the loss of ZIKV infectivity. Increasing virion maturation efficiency modestly increased ZIKV stability, as observed previously with WNV and DENV. Finally, mutations at E residues predicted to confer increased stability to ZIKV did not affect virion half-life. Our results demonstrate that ZIKV is not uniquely stable relative to other flaviviruses, suggesting that its unique pathobiology is explained by an alternative mechanism. PMID:27601578

  19. Description scheme for video editing work

    Science.gov (United States)

    Ruiloba, Rosa I.; Joly, Philippe

    2001-03-01

    This article presents a Description Scheme (DS) to describe the audio-visual documents from the video editing work point of view. This DS is based on edition techniques used in the video edition domain. The main objective of this DS is to provide a complete, modular and extensible description of the structure of the video documents based on editing process. This VideoEditing DS is generic in the sense that it may be used in a large number of applications such as video document indexing and analysis, description of Edit Decision List and elaboration of editing patterns. It is based on accurate and complete definitions of shots and transition effects required for video document analysis applications. The VideoEditing DS allows three levels of description : analytic, synthetic and semantic. In the DS, the higher (resp. the lower) is the element of description, the more analytic (resp. synthetic) is the information. %Phil This DS allows describing the editing work made by editing boards, using more detailed descriptors of Shots and Transition DSs. These elements are provided to define editing patterns that allow several possible reconstructions of movies depending on, for example, the target audience. A part of the video description made with this DS may be automatically produced by the video to shots segmentation algorithms (analytic DSs ) or by editing software, at the same time the edition work is made. This DS gives an answer to the needs related to the exchange of editing work descriptions between editing softwares. At the same time, the same DS provide an analytic description of editing work which is complementary to existing standards for Edit Decision Lists like SMPTE or AAF.

  20. Discovery of Point Mutations in the Voltage-Gated Sodium Channel from African Aedes aegypti Populations: Potential Phylogenetic Reasons for Gene Introgression

    Science.gov (United States)

    Muranami, Yuto; Kawashima, Emiko; Osei, Joseph H. N.; Sakyi, Kojo Yirenkyi; Dadzie, Samuel; de Souza, Dziedzom K.; Appawu, Maxwell; Ohta, Nobuo; Minakawa, Noboru

    2016-01-01

    Background Yellow fever is endemic in some countries in Africa, and Aedes aegpyti is one of the most important vectors implicated in the outbreak. The mapping of the nation-wide distribution and the detection of insecticide resistance of vector mosquitoes will provide the beneficial information for forecasting of dengue and yellow fever outbreaks and effective control measures. Methodology/Principal Findings High resistance to DDT was observed in all mosquito colonies collected in Ghana. The resistance and the possible existence of resistance or tolerance to permethrin were suspected in some colonies. High frequencies of point mutations at the voltage-gated sodium channel (F1534C) and one heterozygote of the other mutation (V1016I) were detected, and this is the first detection on the African continent. The frequency of F1534C allele and the ratio of F1534C homozygotes in Ae. aegypti aegypti (Aaa) were significantly higher than those in Ae. aegypti formosus (Aaf). We could detect the two types of introns between exon 20 and 21, and the F1534C mutations were strongly linked with one type of intron, which was commonly found in South East Asian and South and Central American countries, suggesting the possibility that this mutation was introduced from other continents or convergently selected after the introgression of Aaa genes from the above area. Conclusions/Significance The worldwide eradication programs in 1940s and 1950s might have caused high selection pressure on the mosquito populations and expanded the distribution of insecticide-resistant Ae. aegypti populations. Selection of the F1534C point mutation could be hypothesized to have taken place during this period. The selection of the resistant population of Ae. aegypti with the point mutation of F1534C, and the worldwide transportation of vector mosquitoes in accordance with human activity such as trading of used tires, might result in the widespread distribution of F1534C point mutation in tropical countries

  1. Human hepatocyte growth factor (hHGF-modified hepatic oval cells improve liver transplant survival.

    Directory of Open Access Journals (Sweden)

    Zhu Li

    Full Text Available Despite progress in the field of immunosuppression, acute rejection is still a common postoperative complication following liver transplantation. This study aims to investigate the capacity of the human hepatocyte growth factor (hHGF in modifying hepatic oval cells (HOCs administered simultaneously with orthotopic liver transplantation as a means of improving graft survival. HOCs were activated and isolated using a modified 2-acetylaminofluorene/partial hepatectomy (2-AAF/PH model in male Lewis rats. A HOC line stably expressing the HGF gene was established following stable transfection of the pBLAST2-hHGF plasmid. Our results demonstrated that hHGF-modified HOCs could efficiently differentiate into hepatocytes and bile duct epithelial cells in vitro. Administration of HOCs at the time of liver transplantation induced a wider distribution of SRY-positive donor cells in liver tissues. Administration of hHGF-HOC at the time of transplantation remarkably prolonged the median survival time and improved liver function for recipients compared to these parameters in the other treatment groups (P<0.05. Moreover, hHGF-HOC administration at the time of liver transplantation significantly suppressed elevation of interleukin-2 (IL-2, tumor necrosis factor-α (TNF-α and interferon-γ (IFN-γ levels while increasing the production of IL-10 and TGF-β1 (P<0.05. HOC or hHGF-HOC administration promoted cell proliferation, reduced cell apoptosis, and decreased liver allograft rejection rates. Furthermore, hHGF-modified HOCs more efficiently reduced acute allograft rejection (P<0.05 versus HOC transplantation only. Our results indicate that the combination of hHGF-modified HOCs with liver transplantation decreased host anti-graft immune responses resulting in a reduction of allograft rejection rates and prolonging graft survival in recipient rats. This suggests that HOC-based cell transplantation therapies can be developed as a means of treating severe liver

  2. Content of arsenic, selenium, mercury in the coal, food, clay and drinking water on the Zhaotong fluorosis area, eastern Yunnan Province

    Energy Technology Data Exchange (ETDEWEB)

    Luo Kun-li; Li Hui-jie; Chen Tong-bin (and others) [Chinese Academy of Sciences, Beijing (China). Institute of Geographic Sciences and Natural Resources Research

    2008-03-15

    About 160 samples of coal, corn, capsicum and drinking water were collected from the endemic fluorosis area of Zhenxiong and Weixin County, Zhaotong City of Yunnan Province, to determine the arsenic (As), selenium (Se) and mercury (Hg) content by AAF-800. The study found that the As content in the main coal seam from the Late Permian coal mines in Zhaotong City is 8.84 mg/kg and some civil coal can reach 89.09 mg/kg. The Se and Hg in the coal samples of Late Permian is lower, but Se and Hg are more concentrated in the pyritic coal balls and the pyritic gangue of the coal seam. The As content in corn and capsicum dried by coal-burning is more than 0.7 mg/kg, the natural standard amount of arsenic content permitted in food by China. The Se and Hg content in corn dried by coal-burning is lower than the natural standard of Se and Hg content in food in China but the Se and Hg content of capsicum dried by coal-burning exceeds the amount permitted by the natural standard for food in China. Clay, used as an additive for the coal-burning process and as a binder in making briquettes, contains a high content of As, generally more than 16 mg/kg. However, the Se and Hg content of clay itself are low. The As, Se and Hg content of drinking water are lower than the natural standard of As, Se and Hg content in the drinking water. So, there is high-As content coal and high-As content dried corn and capsicum in the endemic fluorosis area of Zhaotong City of Yunnan Province. The high As content of the dried corn and capsicum might have originated from the high arsenic content of burnt coal and clay. 30 refs., 4 tabs.

  3. Selective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva and Sponge (Haliclona oculata Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Seydi

    2015-12-01

    Full Text Available Background Natural products isolated from marine environments are well known for their pharmacodynamic potential in diverse disease treatments, such as for cancer or inflammatory conditions. Sea cucumbers are marine animals of the phylum Echinoderm and the class Holothuroidea, with leathery skin and gelatinous bodies. Sponges are important components of Persian Gulf animal communities, and the marine sponges of the genus Haliclona have been known to display broad-spectrum biological activity. Many studies have shown that sea cucumbers and sponges contain antioxidants and anti-cancer compounds. Objectives This study was designed to determine the selective toxicity of Persian Gulf sea cucumber (Holothuria parva and sponge (Haliclona oculata methanolic extracts on liver mitochondria isolated from an animal model of hepatocellular carcinoma, as part of a national project that hopes to identify novel potential anticancer candidates among Iranian Persian Gulf flora and fauna. Materials and Methods To induce hepatocarcinogenesis, rats were given diethylnitrosamine (DEN injections (200 mg/kg i.p. by a single dose, and then the cancer was promoted with 2-acetylaminofluorene (2-AAF (0.02 w/w for two weeks. Histopathological evaluations were performed, and levels of liver injury markers and a specific liver cancer marker (alpha-fetoprotein, were determined for confirmation of hepatocellular carcinoma induction. Finally, mitochondria were isolated from cancerous and non-cancerous hepatocytes. Results Our results showed that H. parva methanolic extracts (250, 500, and 1000 µg/mL and H. oculata methanolic extracts (200, 400, and 800 µg/mL increased reactive oxygen species (ROS formation, mitochondrial membrane potential (MMP, mitochondrial swelling, and cytochrome c release in the mitochondria obtained from cancerous hepatocytes, but not in mitochondria obtained from non-cancerous liver hepatocytes. These extracts also induced caspase-3 activation, which is

  4. Effect of Qi-protecting powder (Huqi San) on expression of c-jun, c-fos and c-myc in diethylnitrosamine-mediated hepatocarcinogenesis

    Institute of Scientific and Technical Information of China (English)

    Xia Li; Zheng-Ming Shi; Ping Feng; Zhao-Yang Wen; Xue-Jiang Wang

    2007-01-01

    AIM: To study the inhibitory effect of Huqi San (Qiprotecting powder) on rat prehepatocarcinoma induced by diethylinitrosamine (DEN) by analyzing the mutational activation of c-fos proto-oncogene and over-expression of c-jun and c-myc oncogenes.METHODS: A Solt-Farber two-step test model of prehepatocarcinoma was induced in rats by DEN and 2-acetylaminofluorene (AAF) to investigate the modifying effects of Huqi San on the expression of c-jun, c-fos and c-myc in DEN-mediated hepatocarcinogenesis. Huqi San was made of eight medicinal herbs containing glycoprival granules, in which each milliliter contains 0.38 g crude drugs. γ-glutamy-transpeptidase-isoenzyme (γ-GTase)was determined with histochemical methods. Level of 8-hydroxydeoxyguanosine (OHdG) formed in liver and c-jun, c-fos and c-myc proto-oncogenes were detected by immunohistochemical methods.RESULTS: The level of 8-OHdG, a mark of oxidative DNA damage, was significantly decreased in the liver of rats with prehepatocarcinoma induced by DEN who received 8 g/kg body weight or 4 g/kg body weight Huqi San before (1 wk) and after DEN exposure (4 wk). Huqi Santreated rats showed a significant decrease in number of γ-GT positive foci (P < 0.001, prevention group: 4.96 ±0.72 vs 29.46 ± 2.17; large dose therapeutic group: 7.53± 0.88 vs 29.46 ± 2.17). On the other hand, significant changes in expression of c-jun, c-fos and c-myc were found in Huqi San-treated rats.CONCLUSION: Activation of c-jun, c-fos and c-myc plays a crucial role in the pathogenesis of liver cancer.Huqi San can inhibit the over-expression of c-jun, c-fos and c-myc oncogenes and liver preneolastic lesionsinduced by DEN.

  5. Results of Atrial Fibrillation Ablation in Patients With or Without a History of Atrial Flutter

    Institute of Scientific and Technical Information of China (English)

    Lu chunshan; Liu Xingpeng; Dong Jianzeng; Ma Changsheng

    2006-01-01

    Objectives There are two kind of atrial flutter during circumferential ablation for atrial fibrillation (AF): new onset left atrial flutter (LAFL), with a history of atrial flutter (AFL). What is the relationship of AFL and AF? Whether there are some differences in clinical course and mechanism between the new onset LAFL and the with a history of AFL remained unclear. The aim of this study was to assess the impacts of circumferential ablation on the occurrence of arrhythmias in follow-up in 2 groups:( 1 ) patients with a history of AFL and AF, and (2)patients with new onset LAFL. Methods Data from 465 patients who had circumferential pulmonary vein ablation (CPVA) or segmental pulmonary vein ablation (SPVA) were analyzed. Patients with a history of AFL ablation and patients who had concomitant AFL ablation were included from analysis. Forty-one patients constituted the history of AFL group (group 1, aged 57±13 years, 7 females) and twenty-eight patients constituted the new onset LAFL group (group 2, aged 55±12 years, 6 females). bipolar recordings were obtained from the tricuspid annulus, coronary sinus,interatrial septum and left atrium. Target sites were identified by early, fragmented or double potentials and by concealed entrainment. Linear lesions were created between target sites and nearby anatomical barriers (1) typical atrial flutter (cycle length, 242±39 ms). cavotricuspid isthmus ablation was performed.(2) new onset LAFL (cycle length, 282±153 ms). 20 episodes of AAFs were documented in 20/28 (71.4%)patients. Target sites were identified around pulmonary veins (n=10), gap in linear lesion (n=7), left atrial roof lines (1 case). For those cases the ablation line between PV and mitral annulus was performed.Patients in Group 2 had larger left atria, higher incidence of AFL pre-CPVA, and lower ejection fraction. Results There was no significant difference in post-CPVA AF recurrence between Groups 1 and 2, but AFL incidence after CPVA was higher in Group

  6. Low molecular weight heparin for the prevention of thrombosis after autologous bone marrow stem cell transplantation in animals%低分子肝素预防自体骨髓干细胞移植血栓形成的动物实验研究

    Institute of Scientific and Technical Information of China (English)

    蒋晶超; 韩明子; 刘佰玲; 高福来; 金世柱

    2011-01-01

    目的 探讨低分子肝素预防小鼠自体骨髓单个核细胞移植后血栓形成的作用.方法 应用CCI4/2-AAF制备小鼠急性肝损伤模型,然后行骨髓单个核细胞移植.实验A组于经尾静脉注入骨髓干细胞悬液;实验B组于经尾静脉先注入低分子肝素后注入骨髓干细胞悬液.分别于移植2周后处死小鼠,取2组小鼠心、肺、肝、肾、脑等重要脏器于显微镜下观察有无血栓.结果 HE染色观察发现:A组肝脏、心脏、肺脏、肾脏均有血栓形成(脑未发现血栓);B组肝脏、心脏、肺脏、肾脏及脑均无血栓形成.结论 低分子肝素可预防BALB/c小鼠尾静脉移植BMMCs后导致的血栓形成.%Objective To evaluate the low molecular weight heparin for the prevention of thrombosis after autologous bone marrow mononuclear cell transplantation. Methods Hepatic injury mice models were established by carbon tetra-chloride ( CC14) and administration of 2-acetylminofluorene(2-AAF). Group A; BMMNC were injected via the tail vein; group B; The low molecular weight heparin were injected via the tail vein before the BMMNC transfusion. Two weeks after transplantation, liver, kidney, lung, heart and brain were examined anatomically, and the thrombus in these organs were further observed under the microscope. Results Group A: Thrombous could be found in liver, kidney, lung,heart (not in brain. ) Group B; Thrombous could not be found in liver, kidney, lung, heart and brain. Conclusion The low molecular weight heparin can be used for the prevention of the thrombosis after the autologous bone marrow mononuclear cell transplantation.

  7. 非插入式结肠水疗法治疗脑卒中患者便秘的效果观察及护理%Effect of non-intrusive colon hydrotherapy on constipation in post-stroke patients

    Institute of Scientific and Technical Information of China (English)

    陈洁芬; 李绣球; 高彦平

    2011-01-01

    目的:探讨应用非插人式结肠水疗法治疗脑卒中患者便秘的效果,并总结护理要点.方法:对本科室收治的152例脑卒中便秘患者采用非插入式结肠水疗法通便治疗,并给予针对性护理.观察治疗效果.结果:首次通便有效147例.有效率为96.7%;经过25d治疗.治愈48例(31.6%),好转77例(50.6%),无效27例(17.8%),治疗总有效率为82.2%.结论:应用非插入式结肠水疗法能有效解除脑卒中患者便秘症状,治疗过程安全舒适,值得临床推广应用.%Objective To evaluate the effectiveneas of non-intrusive colon hydrotherapy in treating patients with constipation after stroke and to summarize the nursing points. Methods A total of 152 post-stroke patients with constipation were treated by non-intrusive colon hydrotherapy treatment, and a full range of psychological care was provided. The treatment effectiveness was observed. Results The first time laxative effective was 96.7% (147/152). Forty-eight patients (31.6%) were cured 77 patients (50.6%) were improved, and there was no improvement in 27 patients ( 17.8%) after 25 days of hydrotherapy tre8tment, and the total effective rate was 82.2%. Condusion Non-intrusive colon hydrotherapy and corresponding nursing can effectively treat constipation after stroke and the treatment processes were aafe and comfortable, and deserve clinical application.

  8. 大鼠急性肝损伤后经门静脉自体骨髓单个核细胞移植对宿主凝血机制的影响%Effects of autologous bone marrow mononuclear cell transplantation on the host coagulation sys- tem in rat acute liver injury model

    Institute of Scientific and Technical Information of China (English)

    刘佰玲; 韩明子; 蒋晶超; 高福来; 胡宗晶

    2011-01-01

    目的 观察经门静脉大鼠自体骨髓单个核细胞移植后是否形成肝内及肝外血栓.方法 应用CCl4/2-AAF制备大鼠急性肝损伤模型,从大鼠骨髓分离骨髓单个核细胞.实验A组:经门静脉注射骨髓单个核细胞0.3 mL/只;B组:经门静脉注射生理盐水0.3 mL/只;分别于移植后两周处死大鼠,取心、肝、肺、脑、肾、脾等组织观察大鼠这些器官有无血栓形成.结果 各器官血栓形成情况:HE染色观察移植后2周心、肝、肺、脑、肾均无血栓形成.结论 通过门静脉移植的骨髓单个核细胞在急性肝损伤的大鼠各个器官均无血栓形成.%Objective To observe whether thrombus formation in intrahepatic and extrahepatic vessels after the transplantation of autologous bone marrow mononuclear cell after acute liver injury. Methods Hepatic injury rat models were established by carbon tetrachloide ( CCl4 ) and adnunistration of 2-acetyminofluorene ( 2-AAF ). Bone marrow mononuclear cells (BMMNC) were isolated from the rat' s bone marrow and transplanted to rats in group A; 3 X 10 BMMNC were injected into the portal vein of the rat models; group B: normal saline was injected as controls. Results Thrombus could not be detected in hearts, livers, lungs, kindys, brains and spleens in rats of group A. Conclusion In rats with acute hepatic injury, transplantion of BMMNC via portal vein is safe in consideration of thrombus formation.

  9. Guideline for inspection of plant concerning creep below the limit temperature; Stoed vid besiktning av anlaeggningar avseende krypning under graenstemperaturen

    Energy Technology Data Exchange (ETDEWEB)

    Storesund, Jan; Bjurstroem, Henrik; Weilin Zang; Andersson-Frejd, Ulf

    2007-12-15

    In an earlier report from Vaermeforsk, Inspecta and AaF mapped the extent of the problem of creep damage in several components of steam systems in power plants, which systems operate at temperatures below the lower limit for creep. Recommendations were issued regarding inspection of components where creep damage may occur. The authorities have issued a new regulation, AFS 2005:3 regarding inspection of pressure vessels, extending the requirement for inspection against creep fracture down to 350 deg C. This extensive inspection and testing is though considered as costly by the plants. A simplified inspection routine has been requested so that status regarding creep in exposed components may be determined at a more reasonable cost. In the present follow-up project, the goal was to provide a more comprehensive basis for the recommendations on methods for identifying exposed components. This has included: Collecting information from the replica tests that have been carried out during 2006 and 2007 as a complement to the documentation in the earlier project; Verifying the usability of analyses of elastic stresses; Additional simulations with FEM with the purpose of identifying critical geometries of components and positions. Information has been gathered from 11 replica inspections during the last two years. Creep damage has been observed in every case. In two of them, micro and macro cracks were observed. Eight of the remaining nine had significant occurrences of creep cavities. Damage has occurred also below 450 deg C, which was hitherto unknown. Excessive temperatures may have occurred in one of the two cases; defects and high system stresses are the probable causes of the other case. Positions where the most severe creep damage was observed were not the same as those with the highest stresses in the elastic stresses analysis. Therefore, components which are prone to damage according to experience, e.g. T-joints, should also be tested, even if the utilisation is

  10. NASA Tech Briefs, November 2008

    Science.gov (United States)

    2008-01-01

    Topics covered include: Digital Phase Meter for a Laser Heterodyne Interferometer; Vision System Measures Motions of Robot and External Objects; Advanced Precipitation Radar Antenna to Measure Rainfall From Space; Wide-Band Radar for Measuring Thickness of Sea Ice; Vertical Isolation for Photodiodes in CMOS Imagers; Wide-Band Microwave Receivers Using Photonic Processing; L-Band Transmit/Receive Module for Phase-Stable Array Antennas; Microwave Power Combiner/Switch Utilizing a Faraday Rotator; Compact Low-Loss Planar Magic-T; Using Pipelined XNOR Logic to Reduce SEU Risks in State Machines; Quasi-Optical Transmission Line for 94-GHz Radar; Next Generation Flight Controller Trainer System; Converting from DDOR SASF to APF; Converting from CVF to AAF; Documenting AUTOGEN and APGEN Model Files; Sequence History Update Tool; Extraction and Analysis of Display Data; MRO DKF Post-Processing Tool; Rig Diagnostic Tools; MRO Sequence Checking Tool; Science Activity Planner for the MER Mission; UAVSAR Flight-Planning System; Templates for Deposition of Microscopic Pointed Structures; Adjustable Membrane Mirrors Incorporating G-Elastomers; Hall-Effect Thruster Utilizing Bismuth as Propellant; High-Temperature Crystal-Growth Cartridge Tubes Made by VPS; Quench Crucibles Reinforced with Metal; Deep-Sea Hydrothermal-Vent Sampler; Mars Rocket Propulsion System; Two-Stage Passive Vibration Isolator; Improved Thermal Design of a Compression Mold; Enhanced Pseudo-Waypoint Guidance for Spacecraft Maneuvers; Altimetry Using GPS-Reflection/Occultation Interferometry; Thermally Driven Josephson Effect; Perturbation Effects on a Supercritical C7H16/N2 Mixing Layer; Gold Nanoparticle Labels Amplify Ellipsometric Signals; Phase Matching of Diverse Modes in a WGM Resonator; WGM Resonators for Terahertz-to-Optical Frequency Conversion; Determining Concentration of Nanoparticles from Ellipsometry; Microwave-to-Optical Conversion in WGM Resonators; Four-Pass Coupler for Laser

  11. Thermal Structure of Jupiter's Infrared Hotspots and Plumes in the Northern Equatorial Region

    Science.gov (United States)

    Fletcher, Leigh N.; Orton, Glenn S.; Rogers, John H.; Greathouse, Thomas K.; Momary, Thomas W.; Giles, Rohini Sara; Melin, Henrik; Sinclair, James; Irwin, Patrick Gerard Joseph; Vedovato, Marco

    2016-10-01

    The most prominent features of Jupiter's northern equatorial region are the visibly dark, 5-µm-bright 'hotspots' that move rapidly eastward on the southern edge of the North Equatorial Belt (NEB, Allison 1990, doi:10.1016/0019-1035(90)90069-L). We combine high-resolution thermal-infrared (5-20 µm) imaging from VLT/VISIR and IRTF/SpeX with spatially resolved spectroscopy from IRTF/TEXES to examine the thermal and chemical conditions in the equatorial region during the 2015-2016 apparition. The high spatial resolution permits the first detailed cross-comparison of thermal and visible-albedo conditions within the hotspots. We find that: (i) cloud-clearing within the hotspots creates 8.6-µm bright patches that are broader and more diffuse than their 5-µm counterparts; (ii) cloudy, cool cells ("plumes") in the northern Equatorial Zone are ammonia-rich and dark in the 5- and 8-12 µm range; (iii) the hotspots sometimes demonstrate a westward tilt with altitude in the 0.1-0.8 bar region (Fletcher et al., 2016, doi:10.1016/j.icarus.2016.06.008); and (iv) blue-grey streaks on the southeastern edges of these ammonia-rich cells are also cloud free and bright at 5-12 µm. This regular longitudinal pattern of cloudy cells and cloud-free hotspots is consistent with condensation of NH3-rich air as it ascends in cells, and subsidence of dry, volatile-depleted air in the hotspots. The westward tilt of the NEB hotspots with height that was detected in 2014 (but not in 2016) supports the equatorial Rossby-wave hypothesis for the NEB pattern. This equatorial wave is distinct from those in the upper troposphere during the 2015-16 NEB expansion event (Orton et al., DPS/EPSC 2016). The cells and hotspots observed in the thermal-IR are the same type as those detected at near-IR wavelengths by Galileo/NIMS (Baines et al. 2002, doi:10.1006/icar.2002.6901) and in the radio, probing the deep atmosphere (de Pater et al., 2016, doi:10.1126/science.aaf2210), suggesting a coherent structure

  12. Applications of Acorus in Water Pollution Control%菖蒲属植物在水污染治理中的应用

    Institute of Scientific and Technical Information of China (English)

    任启飞; 龙成昌; 周庆; 姚松林; 房小晶

    2013-01-01

    水污染是目前面临的一类主要的环境问题之一,利用水生高等植物治理污染水体的方法正逐渐受到人们的关注.菖蒲属植物在我国各地均有分布,该属的菖蒲和石菖蒲已被广泛运用于污染水体的治理.综述了菖蒲属植物净化污染水体的主要功能及研究进展.菖蒲属植物对富营养化水体中的TP、TN、NH3-N等指标具有显著的去除效果,对富营养化水体中藻类生长有显著的抑制效果.同时,对Cu、Pb、Cr、Zn等多种重金属的吸收富集效果明显,对萘、芴、蒽、芘等有机污染物具有一定的吸收效果.此外,菖蒲与茭白、酸模、鸢尾等组合使用可提高污水净化效率.说明,菖蒲属植物可作为治理水体污染的重要物种,对治理污水具有较好的技术经济价值.%Water pollution is one of the major environmental issues, higher plants for purifying sewage attract many researchers. Acorus are widely distributed in China. A. calamus and A. tatarinoxvn has been widely applied in polluted water management. The authors summarized the applications of Acorus in water pollution control. It shows that Acorus has obvious effect in absorbing TN" TP, NH3-N in eutrophic water and has a significant restraint effect on the growth of algae; It also has obvious effect in absorbing heavy metals such as Cu, Pb, Cr. Zn and and has certain removal effect about organic pollutants such as naphthalene, anthracene, pyrene and AAF. In addition,The combination of Acorus, Wildrice , Rumex and Iris can improve sewage purifying efficiency. So Acorus is one of the most important species in water pollution control, Using Acorus for purifying polluted water has better technical and economic value.

  13. Activin A suppressed hepatic oval cell-mediated liver regeneration in vivo%Activin A抑制卵圆细胞介导肝再生的体内研究

    Institute of Scientific and Technical Information of China (English)

    陈琳; 周巧丹; 丁则阳; 张伟; 张必翔; 梁慧芳; 陈孝平

    2012-01-01

    Objective To study the inhibitory effect and mechanism of Activin A on hepatic oval cell-mediated liver regeneratioa Methods 2-acetylaminofluorene/partial hepatectomy (2-AAF/PH) o-val cell-mediated liver regeneration model was established. One μg Activin A (Activin A group) or normal saline (control S group) was infused into portal vein immediately after 70 % partial hepatectomy. Animals were sacrificed at 2nd, 4th, 6th, and 9th day after hepatectomy. Liver regeneration rate was measured. Changes in Pan-CK positive oval cell number and nuclear bromodeoxyuridine labeling were detected by using immunohistochemistry. The expression of phosphorylated smad2, p15, and p21 were detected by using Western blotting. Results As compared with control group, the Pan-CK positive number was significantly reduced in Activin A group at 6th and 9th day after hepatectomy,and Brdu labeling nuclear number was reduced in Activin A group at 4th,6th and 9th day after hepatectomy. The liver regeneration rate in Activin A group was lower than in control group. Higher levels of phosphorylated smad2,p15 and p21 were detected in Activin A group. Conclusion Activin A could suppress o-val cell-mediated liver regeneration through smad pathway-dependent up-regulation of p15 and p21.%目的 研究Activin A对卵圆细胞介导肝再生的抑制作用及其机制.方法 建立2-乙酰胺基芴/部分肝切除卵圆细胞介导肝再生模型,肝切除后立即经门静脉注射1 μg Activin A(Activin A组),或生理盐水(对照组).肝切除后不同时间点取肝脏标本检测卵圆细胞增殖及肝再生率和磷酸化smad2,p15和p21的表达.结果 Activin A组肝脏中卵圆细胞的增殖及肝再生率均明显低于对照组;Activin A组肝脏中smad2的磷酸化水平及p15,p21的表达水平均高于对照组.结论 Activin A可以通过smad信号通路抑制卵圆细胞介导的肝再生.

  14. Development of a Rapid Gold Immunochromatographic Strip Test for the Diagnosis of Foot-and-Mouth Disease Virus O,A and Asia I Type%口蹄疫病毒O、A、Asia I型定型诊断胶体金免疫层析方法的建立

    Institute of Scientific and Technical Information of China (English)

    蒋韬; 梁仲; 陈涓; 何继军; 吕律; 马维民; 刘在新; 刘湘涛

    2008-01-01

    [目的]建立一种从田问样品中快速、灵敏、简便检测O、A、Asia I型口蹄疫病毒抗原的胶体金免疫层析方法(GICA).[方法]采用柠檬酸三钠还原制备胶体金颗粒,标记纯化的标准株O、A、Asia I型口蹄疫抗体.将纯化的口蹄疫流行株O/China99,A/AF72 and Asia I/China05抗体和羊抗豚鼠抗体分别包被在硝酸纤维素膜上,作为检测带与质控带.经试验条件优化,组装形成胶体金定型诊断试剂盒.[结果]本试验研制的口蹄疫定型试剂盒具有良好的灵敏度,可检测到7.8×104 LD50(1:128倍稀释乳鼠毒)的病毒量.同时对阳性及阴性样品进行3次重复检测结果完全相同,说明其有较好的重复性;特异性试验证实该方法在检测口蹄疫临床症状相似病原,如猪水疱病抗原(SVD)、水泡性12炎(VS)、水泡性疹(VES)等病毒时无交叉反应;试剂盒对206份已知血清型田间及实验室样品的符合率试验,O、A、Asia I型和阴性样本的符合率分别为92.45%、91.66%、92.75%、100%定型准确率94.58%.试剂盒在4℃下可保存6个月.[结论]本试验研发的12蹄疫病毒胶体金定型诊断试剂盒是一种快速、灵敏、特异的FMD抗原检测方法,对基层现场具有广泛应用价值.

  15. Mitsuda's reactions: induced by BCG in the normal Rhesus ("Macacca mulatta"

    Directory of Open Access Journals (Sweden)

    M. J. Pereira Filho

    1955-12-01

    the repetition of the tests, even though the intensity of the reaction always remains the same. This precocious reaction (Fernandez type occurs both shortly and long time after the application of the BCG. Its precocity depends not of the antigen only because the first Mitsuda's reaction after the BCG application occurs after some time and seems not influenced by the control lepromin test effected on the Rhesus before the BCG. 5 On the control group, the animals which received a.a.f. bacilli suspensions (Mycobacterium sp.; M. avium, and M. smegmatis, did not show reverseals of the Mitsuda's reaction. Two Rhesus, however, which received dead BCG (120ºC autoclave 1 hour, one intradermically (0.006 g and the other orally (1.2 g, did both present reversals of the Mitsuda's reaction, with weak positivity (+. In all animals of the control-group, the allergic reactions were found negative. 6 Strong local inflammatory reactions were observed in the Rhesus that had received living BCG by intradermal via, and in the one submitted to multipunctures, there occurred the formation of a large caseous abcess. 7 The allergic tuberculinic and infratuberculinic reactions appeared dissociated from the Mitsuda's reactions: sometimes they are more precocious, occurring before of the lepromin test; on other occasions they disappear, when the Mitsuda's reactions still persist; and finally, they may be absent, when the latter occur, especially after the oral application of the BCG. 8 In Rhesus which received BCG by testicular and peritonela via, in the infratuberculinic test (0.1 ml of total BCG extract, besides the classic answer, which occurs between 48 and 96 hours, one could observe a delayed answer (15 to 20 days, represented by a non-erythematous nodule, which persists for 11-14 days.

  16. Analysis on S-gene quasispecies sequence of patients with simultaneously positive HBsAg and Anti-HBs%HBsAg与抗-HBs同时阳性者HBV S基因准种分析

    Institute of Scientific and Technical Information of China (English)

    张硕; 沈立萍; 张爽; 王锋; 张勇; 毕胜利

    2014-01-01

    目的 分析HBsAg与抗-HBs同时阳性者体内乙型肝炎病毒(HBV)S基因准种分布及基因突变情况.方法 收集3例HBsAg与抗_HBs同时阳性者的血清和基本信息,提取HBV DNA,用PCR法扩增HBV S基因,将S基因克隆至载体,测序后通过软件分析准种序列差异、计算遗传距离分析进化选择压力.结果 1号样本感染的HBV为B2型,2号和3号为C5型;准种遗传进化分析发现1号同时存在两种优势病毒株,2号和3号只有一种优势病毒株;三者的信息熵水平无统计学差异;选择压力分析显示1号样本病毒种群的Ka<Ks,ω=0.58,受到纯化选择压力;2号发生AAF134S、AAS143P置换突变,3号发生AAT125A置换突变和ntG494A、ntC500A无义突变.结论 HBsAg与抗_HBs同时阳性者S基因存在复杂的准种多样性;在准种中发现少量a抗原决定簇关键位点突变毒株,通过准种分析研究能真实地体现HBV生存状态.%Objective To analyze distribution and mutation of HBV quasispecies heterogeneity in patients with concurrent existence of HBsAg and HBsAb in serum.Methods Three HBsAg and Anti-HBs simultaneously positive carriers who received whole course timely vaccination were analyzed.The HBV DNA was extracted from the serum,S gene was amplified by PCR assay,then cloned and sequenced.Entropy,genetic distances,nosynonymous (Ka) and synonymous (Ks) substitution rates and molecular phylogenetics,were evaluated for evolutionary analysis.Results The genotype of HBV in first sample was B2 and in other samples was C5.Two kinds of predominant quasispecies were observed in first sample base on genetic distances and quasispecies distribution.One predominant quasispecies were observed in other samples.Entropy values of three samples had no significant difference.The ratios of Ka/Ks of first sample were lower than those of the other samples with ω =0.58 (Ka < Ks).The quasispecties in first sample were under negative (purifying) selection pressure.The point

  17. Airborne hygrometer calibration inter-comparison against a metrological water vapour standard

    Science.gov (United States)

    Smorgon, Denis; Boese, Norbert; Ebert, Volker

    2014-05-01

    Water vapour is the most important atmospheric greenhouse gas, which causes a major feedback to warming and other changes in the climate system. Knowledge of the distribution of water vapour and its climate induced changes is especially important in the upper troposphere and lower stratosphere (UT/LS) where vapour plays a critical role in atmospheric radiative balance, cirrus cloud formation, and photochemistry. But, our understanding of water in the UT/LS is limited by significant uncertainties in current UT/LS water measurements. One of the most comprehensive inter-comparison campaigns for airborne hygrometers, termed AQUAVIT (AV1) [1], took place in 2007 at the AIDA chamber at the Karlsruhe Institute of Technology (KIT) in Germany. AV1 was a well-defined, referred, blind inter-comparison of 22 airborne field instruments from 17 international research groups. One major metrological deficit of AV1, however, was, that no traceable reference instrument participated in the inter-comparison experiments and that the calibration procedures of the participating instruments were not monitored or interrogated. Consequently a follow-up inter-comparison was organized in April 2013, which for the first time also provides a traceable link to the international humidity scale. This AQUAVIT2 (AV2) campaign (details see: http://www.imk-aaf.kit.edu/aquavit/index.php/Main_Page) was again located at KIT/AIDA and organised by an international organizing committee including KIT, PTB, FZJ and others. Generally AV2 is divided in two parallel comparisons: 1) AV2-A uses the AIDA chamber for a simultaneous comparison of all instruments (incl. sampling and in-situ instruments) over a broad range of conditions characteristic for the UT/LS; 2) AV2-B, about which this paper is reporting, is a sequential comparison of selected hygrometers and (when possible) their reference calibration infrastructures by means of a chilled mirror hygrometer traced back to the primary National humidity standard

  18. 不同移植途径对大鼠骨髓干细胞迁移至肝脏及分化的影响%Study on migration and differentiation of rat bone marrow stem cells to hepatocytes under three different routes of transplantation

    Institute of Scientific and Technical Information of China (English)

    耿莹; 韩明子; 洪钰; 金世柱; 孙立影

    2008-01-01

    Objective To observe the migration and differentiation of rat bone rnarmw stem cells to liver under three different routes of transplantation.Methods Intragastric administration was taken to 30 SD rats with CCl4-AAF by which the acute hepatic injured models were made.Bone marrow mononuclear cells which were lsolated from bone marrow with lymphocyte separating medium were labeled by PKH26.The rats were divided into 3 groups.The rats in portal vein-transDlanted group anesthetized were autotransplanted with 0.4 ml bone marrow stem cells suspension via portal vein.The rats in tail vein-transplanted group were autotransplanted with 0.4 ml bone marrow stam cells suspenslon via tail vein.The central sections were made on the abdomen of the rats in hepatic artery-transplanted group to expose the hepatic artery.The distal end of the gastroduodenal arterv was ligated and the blood flow of the common hepatic artery was cut by clamp 0.4 ml bone marrow stem cells suspension was injected In front of ligation of the gastroduodenal artery.Two weeks after transPlantion,the liver tissues were taken from the rats of every group and cut into frozen sections.The immunohistochemistry staining by fluorescein isothiocyanate was done to the sections for detection of the PKH26-labeled cells and the expression of albumin and CK18.Results Red fluorescence of PKH26 in the sections of every group was observed.The number of PKH26-labeled cells in the liver of portal vein-transplanted group,tail vein-transplanted group and hepatic artery-transplanted group was(58.0±2.67),(57.8±3.04)and(58.3±3.52)respectively.There was no significant difference between the groups(P>0.05).The sections in every group expressed albumin and CK18(green fluorescence).The cells expressing red and green fluorescence at the same time were observed under fluorescence microscope(the yellow fluorescence=red fluorescence+the green fluorescence).Conclusion Under injured liver status,bone marrow stem cells of three groups

  19. Aespoe Hard Rock Laboratory. Sensor Data Report No 23

    Energy Technology Data Exchange (ETDEWEB)

    Goudarzi, Reza; Johannesson, Lars-Erik (Clay Technology AB (Sweden))

    2010-11-15

    The Prototype Repository Test consists of two sections. The installation of the first Section of Prototype Repository was made during summer and autumn 2001 and Section 2 was installed in spring and summer 2003. This report presents data from measurements in the Prototype Repository during the period 20010917-20100601. The report is organized so that the actual measured results are shown in Appendix 1-10, where Appendix 8 deals with measurements of canister displacements (by AITEMIN), Appendix 9 deals with geo-electric measurements in the backfill (by GRS), Appendix 10 deals with stress and strain measurement in the rock (by AaF) and Appendix 11 deals with measurement of water pressure in the rock (by VBB/VIAK). The main report and Appendix 1-7 deal with the rest of the measurements. Section 1. The following measurements are made in the bentonite in each of the two instrumented deposition holes in Section 1 (1 and 3): Temperature is measured in 32 points, total pressure in 27 points, pore water pressure in 14 points and relative humidity in 37 points. Temperature is also measured by all relative humidity gauges. Every measuring point is related to a local coordinate system in the deposition hole. The following measurements are made in the backfill in Section 1. Temperature is measured in 20 points, total pressure in 18 points, pore water pressure in 23 points and relative humidity in 45 points. Temperature is also measured by all relative humidity gauges. Furthermore, water content is measured by an electric chain in one section. Every measuring point is related to a local coordinate system in the tunnel. The following measurements are made on the surface of the canisters in Section 1: Temperature is measured every meter along two fiber optic cables. Furthermore, displacements of the canister in hole 3 are measured with 6 gauges. The following measurements are made in the rock in Section 1: Temperature is measured in 37 points in boreholes in the floor. Water

  20. Polimorfismo Val247Leu do gene β2-glicoproteína 1 pode justificar a gênese de anticorpos antiβ2GP1 e síndrome do anticorpo antifosfolípide na hanseníase multibacilar Val247Leu polymorphism of β2 glycoprotein 1 gene may justify the genesis of anti β2GP1 antibodies and Antiphospholipid Syndrome in Multibacillary Leprosy

    Directory of Open Access Journals (Sweden)

    Maria José Franco Brochado

    2009-08-01

    Full Text Available FUNDAMENTOS - Anticorpos antifosfolípides (AAF, como antiβ2GP1 (β2-glicoproteína 1, são descritos na hanseníase multibacilar (MB sem, contudo, caracterizar a síndrome do anticorpo antifosfolípide (SAF, constituída por fenômenos tromboembólicos (FTE. A mutação Val247Leu no V domínio da β2GP1 - substituição da leucina por valina - expõe epítopos crípticos com consequente formação de anticorpos antiβ2GP1. OBJETIVO: Avaliar a associação do polimorfismo Val247Leu do gene β2GP1 com títulos de anticorpos antiβ2GP1 na hanseníase. MÉTODO: O polimorfismo Val247Leu foi detectado por PCR-RFLP, e os títulos de anticorpos antiβ2GP1, por Elisa. RESULTADOS: O genótipo Val/Val estatisticamente predominou no grupo de hansênicos, em relação ao controle. Embora maiores títulos de anticorpos antiβ2GP1 IgM estivessem alocados no grupo MB com genótipos Val/Val e Val/Leu, não houve diferença estatística em relação ao genótipo Leu/Leu. Dos sete pacientes MB com FTE, quatro apresentaram heterozigose, e três Val/Val homozigose. CONCLUSÃO: A prevalência do genótipo Val/Val no grupo de hansênicos pode justificar parcialmente a presença de anticorpos antiβ2GP1 na forma MB. A heterozigose ou homozigose Val/Val nos sete pacientes com hanseníase MB e FTE corroboram a implicação de expressão fenotípica anômala da β2GPl e formação de anticorpos antiβ2GPl, com consequente FTE e SAF.BACKGROUND - Multibacillary (MB leprosy may be manifested with antiphospholipid antibodies (aPL, among which anti-β2GP1 (β2-glycoprotein 1. High titers of aPL are associated with APS (Antiphospholipid Syndrome, characterized by thrombosis. The mutation Val247Leu in the domain V of β2GP1 exposes hidden epitopes with consequent development of anti-β2GP1 antibodies. OBJECTIVE: To evaluate the Val247Leu polymorphism of β2GP1 gene and its correlation with anti-β2GP1 antibodies in leprosy patients. METHODS: The Val247Leu polymorphism was