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Sample records for aaa cavendish cv

  1. Antibacterial and Antioxidant Activities of Banana (Musa, AAA cv. Cavendish Fruits Peel

    Directory of Open Access Journals (Sweden)

    Matook S. Mokbe

    2005-01-01

    Full Text Available The fresh green and yellow banana peel of, (Musa, cv. Cavendish fruits were treated with 70% acetone, which were partitioned with chloroform (CHCl3 and ethyl acetate (EtOAc, sequentially. The antioxidant activities of the extracts were evaluated by using the thiocyanate method, ß-carotene bleaching method and 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical elimination. While, antimicrobial activities of the extracts and isolated components were evaluated using paper disc methods and Minimum Inhibition Concentration (MIC. The EtOAc and water soluble fractions of green peel displayed high antimicrobial and antioxidant activity, respectively. Antioxidant activity of water extracts was comparable to those of synthetic antioxidants such as butylated hydroxyanisole and butylated hydroxytoluene. Among all isolated components ß-sitosterol, malic acid, succinic acid, palmatic acid, 12-hydroxystrearic acid, glycoside, the d-malic and 12-hydroxystrearic acid were the most active against all the Gram-negative and positive bacterial species tested. The MIC of d-malic and succinic acid was varying between 140-750 ppm, respectively.

  2. Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish).

    Science.gov (United States)

    Wang, Zhuo; Jia, Caihong; Li, Jingyang; Huang, Suzhen; Xu, Biyu; Jin, Zhiqiang

    2015-01-01

    Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubens (Foc) is the most serious disease that attacks banana plants. Salicylic acid (SA) can play a key role in plant-microbe interactions. Our study is the first to examine the role of SA in conferring resistance to Foc TR4 in banana (Musa acuminata L. AAA group, cv. Cavendish), which is the greatest commercial importance cultivar in Musa. We used quantitative real-time reverse polymerase chain reaction (qRT-PCR) to analyze the expression profiles of 45 genes related to SA biosynthesis and downstream signaling pathways in a susceptible banana cultivar (cv. Cavendish) and a resistant banana cultivar (cv. Nongke No. 1) inoculated with Foc TR4. The expression of genes involved in SA biosynthesis and downstream signaling pathways was suppressed in a susceptible cultivar and activated in a resistant cultivar. The SA levels in each treatment arm were measured using high-performance liquid chromatography. SA levels were decreased in the susceptible cultivar and increased in the resistant cultivar. Finally, we examined the contribution of exogenous SA to Foc TR4 resistance in susceptible banana plants. The expression of genes involved in SA biosynthesis and signal transduction pathways as well as SA levels were significantly increased. The results suggest that one reason for banana susceptibility to Foc TR4 is that expression of genes involved in SA biosynthesis and SA levels are suppressed and that the induced resistance observed in banana against Foc TR4 might be a case of salicylic acid-dependent systemic acquired resistance.

  3. Molecular Cloning and Expression of MaNPR1E Gene from Banana (Musa acuminata L. AAA group, cv. Cavendish)%香蕉NPR 1E 基因的克隆及表达分析

    Institute of Scientific and Technical Information of China (English)

    王卓; 徐碧玉; 贾彩红; 李健平; 刘菊华; 张建斌; 苗红霞; 金志强

    2015-01-01

    NPR1基因可参与调节植物对病原菌的广谱抗性,在植物系统抗性中起着关键的调控作用。通过RACE (rapid-amplification of cDNA ends)方法从香蕉根系中获得NPR1E基因,命名为MaNPR1E(GenBank登录号分别为: KF582550)。 MaNPR1E是香蕉NPR1基因编码框全长cDNA,包含一个1755 bp的最大开放阅读框,编码一个含584个氨基酸的蛋白质。经蛋白质序列同源比对发现,其含有完整的BTB/POZ结构域、锚蛋白重复ANK序列和NPR1-like-C结构域,属于典型的NPR1蛋白。系统进化树比对分析表明, MaNPR1E与海枣PdNPR3(XP_008808341.1)和油棕EgNPR3(XP_010914123.1)的亲缘关系较近。组织特异性研究表明,该基因组成型表达于香蕉各组织。实时荧光定量PCR分析表明,接种枯萎病菌后MaNPR1E的表达在感病品种中被抑制,而在抗病品种中被激活; MaNPR1E的表达受乙烯利和水杨酸的诱导。上述结果表明, MaNPR1E可能在香蕉抗枯萎病过程中扮演重要的调控角色。%NPR1(nonexpressor of PR gene 1), involved in regulating plant broad-spectrum resistance, plays important roles in plant system resistance. In this study, we report the molecular characteristics of NPR1E gene cloned from banana(Musa acuminate L. AAA group, cv. Cavendish)using a RACE-PCR-based strategy. MaNPR1E(accession number: KF582550) contained an open reading frame of 1 755 bp which encoded a polypeptide of 584 amino acids. Protein alignment showed that they contain the complete typical conserved BTB/POZ (BR-C, ttk and bab/Pox virus and Zinc finger)domain, ankyrin repeats and NPR1/NIM1 like defence protein C terminal, which belongs to a typical NPR1 protein. Phylogenetic analysis showed that the deduced amino acid sequences of MaNPR1E also have high similarity to PdNPR3 (XP_008808341.1) and EgNPR3 (XP_010914123.1) from Phoenix dactylifera and Elaeis guineensis, respectively. Tissue-specific studies showed that the expression of MaNPR1E was constitutive

  4. Chemical and microbiological interactions between soils and roots in commercial banana plantations (Musa AAA, cv. Cavendish)

    NARCIS (Netherlands)

    Segura Mena, R.; Serrano, E.; Pocasangre, L.; Acuna, O.; Bertsch, F.; Stoorvogel, J.J.; Sandoval, J.A.

    2015-01-01

    A study was performed to determine the relationships between soil chemical and microbiological con-ditions and how they impact soil production. The study was carried out on six Costa Rican commercialbanana farms with high, medium and low productivity. In each of the farms sector with relatively good

  5. Comparative biochemical analysis after steam pretreatment of lignocellulosic agricultural waste biomass from Williams Cavendish banana plant (Triploid Musa AAA group).

    Science.gov (United States)

    Kamdem, Irénée; Jacquet, Nicolas; Tiappi, Florian Mathias; Hiligsmann, Serge; Vanderghem, Caroline; Richel, Aurore; Jacques, Philippe; Thonart, Philippe

    2015-11-01

    The accessibility of fermentable substrates to enzymes is a limiting factor for the efficient bioconversion of agricultural wastes in the context of sustainable development. This paper presents the results of a biochemical analysis performed on six combined morphological parts of Williams Cavendish Lignocellulosic Biomass (WCLB) after steam cracking (SC) and steam explosion (SE) pretreatments. Solid (S) and liquid (L) fractions (Fs) obtained from SC pretreatment performed at 180°C (SLFSC180) and 210°C (SLFSC210) generated, after diluted acid hydrolysis, the highest proportions of neutral sugar (NS) contents, specifically 52.82 ± 3.51 and 49.78 ± 1.39%w/w WCLB dry matter (DM), respectively. The highest proportions of glucose were found in SFSC210 (53.56 ± 1.33%w/w DM) and SFSC180 (44.47 ± 0.00%w/w DM), while the lowest was found in unpretreated WCLB (22.70 ± 0.71%w/w DM). Total NS content assessed in each LF immediately after SC and SE pretreatments was less than 2%w/w of the LF DM, thus revealing minor acid autohydrolysis consequently leading to minor NS production during the steam pretreatment. WCLB subjected to SC at 210 °C (SC210) generated up to 2.7-fold bioaccessible glucan and xylan. SC and SE pretreatments showed potential for the deconstruction of WCLB (delignification, depolymerization, decrystallization and deacetylation), enhancing its enzymatic hydrolysis. The concentrations of enzymatic inhibitors, such as 2-furfuraldehyde and 5-(hydroxymethyl)furfural from LFSC210, were the highest (41 and 21 µg ml(-1), respectively). This study shows that steam pretreatments in general and SC210 in particular are required for efficient bioconversion of WCLB. Yet, biotransformation through biochemical processes (e.g., anaerobic digestion) must be performed to assess the efficiency of these pretreatments.

  6. Effect of physiological harvest stages on the composition of bioactive compounds in Cavendish bananas.

    Science.gov (United States)

    Bruno Bonnet, Christelle; Hubert, Olivier; Mbeguie-A-Mbeguie, Didier; Pallet, Dominique; Hiol, Abel; Reynes, Max; Poucheret, Patrick

    2013-04-01

    The combined influence of maturation, ripening, and climate on the profile of bioactive compounds was studied in banana (Musa acuminata, AAA, Cavendish, cv. Grande Naine). Their bioactive compounds were determined by the Folin-Ciocalteu assay and high-performance thin layer chromatographic (HPTLC) method. The polyphenol content of bananas harvested after 400 degree days remained unchanged during ripening, while bananas harvested after 600 and 900 degree days exhibited a significant polyphenol increase. Although dopamine was the polyphenol with the highest concentration in banana peels during the green developmental stage and ripening, its kinetics differed from the total polyphenol profile. Our results showed that this matrix of choice (maturation, ripening, and climate) may allow selection of the banana (M. acuminata, AAA, Cavendish, cv. Grande Naine) status that will produce optimal concentrations of identified compounds with human health relevance.

  7. Effect of physiological harvest stages on the composition of bioactive compounds in Cavendish bananas

    Institute of Scientific and Technical Information of China (English)

    Christelle BRUNO BONNET; Olivier HUBERT; Didier MBEGUIE-A-MBEGUIE; Dominique PALLET; Abel HIOL; Max REYNES; Patrick POUCHERET

    2013-01-01

    The combined influence of maturation,ripening,and climate on the profile of bioactive compounds was studied in banana (Musa acuminata,AAA,Cavendish,cv.Grande Naine).Their bioactive compounds were determined by the Folin-Ciocalteu assay and high-performance thin layer chromatographic (HPTLC) method.The polyphenol content of bananas harvested after 400 degree days remained unchanged during ripening,while bananas harvested after 600 and 900 degree days exhibited a significant polyphenol increase.Although dopamine was the polyphenol with the highest concentration in banana peels during the green developmental stage and ripening,its kinetics differed from the total polyphenol profile.Our results showed that this matrix of choice (maturation,ripening,and climate) may allow selection of the banana (M.acuminata,AAA,Cavendish,cv.Grande Naine) status that will produce optimal concentrations of identified compounds with human health relevance.

  8. 巴西香蕉苗静置水培营养液配方的初步筛选%Screening of the Nutrient Solution Formulas for Static Hydroponics of Banana (Musa AAA Cavendish Subgroup 'Brazil') Plantlets

    Institute of Scientific and Technical Information of China (English)

    黄小娟; 杨腊英; 谢德啸; 周端咏; 魏巍; 郭利佳; 黄俊生

    2012-01-01

    采用静置水培法,使用4种不同的营养液配方,并以清水培养和椰糠基质栽培为对照,对巴西香蕉苗进行生长比较试验。结果表明:在香蕉苗增加的生物量方面,BXM配方(巴西木植物营养液)处理与椰糠栽培的差异不显著,但与日本园试配方、Hoagland植物营养液配方、1/2 MS培养基营养元素配方和清水培养的差异显著;在株高和叶面积方面,均以椰糠栽培的最大,BXM配方处理的居次,接下来依次是Hoagland植物营养液配方、日本园试配方、1/2 MS培养基配方和清水培养。综上所述,在这4种营养液配方中,BXM配方是巴西香蕉苗生长的最适营养液配方。%In order to study water culture techniques, growths of the banana (Musa AAA cavendish subgroup cv. Brazil) plantlets under the static culture and substrate culture were 'compared. Four nutrient solutions including Hoagland solution, Murashige and Skoog (1/2MS) solution, BXM solution and RSYY solution were used to culture banana plantlets, which grew in tap water or in substrate culture (pearlite as substrate). The data showed that banana plantlets grew well in the four tested nutrient solutions, and there was a significant increase in biomass of the banana plantlets grew in static nutrient solution over that in tap water. Among the four nutrient solutions, the biomass of the banana plantlets that grew in BXM solution was next to that in substrate (pearlite) culture, and was significantly more than that in Hoagland solution, 1/2MS solution and RSYY solution. The indexes such as plant height and leaf area of banana plantlets that grew in BXM solution were largest among the four nutrient solutions, and then that grew in Hoagland solution, RSYY solution and MS solution reduced in turn. In conclusion, the banana plantlets grew best in BXM solution among the four nutrient solutions. So BXM solution is suggested, when growing banana plantlets in water culture.

  9. Mineral fertilizers improve the sensory quality of East African Highland bananas (Musa AAA-EA, cv. Kisansa)

    NARCIS (Netherlands)

    Taulya, G.; Asten, van P.J.A.; Nowakunda, K.; Kaddu-Mukasa, P.

    2010-01-01

    Some farmers in Uganda believe that fertilizers negatively affect the sensory attributes of cooking type bananas. This belief may hamper the adoption of fertilizers. To verify the validity of this belief, bunches (Musa AAA-EA, cv. ‘Kisansa’) from fertilized (i.e. N-P-K-Mg-Zn-S-B-Mo) and non-fertiliz

  10. Avaliação de clones de bananeira do subgrupo Cavendish (Musa acuminata, AAA em Cruz das Almas-BA Evaluation of banana Cavendish clones in Cruz das Almas City, Bahia State, Brazil

    Directory of Open Access Journals (Sweden)

    Sebastião de Oliveira e Silva

    2004-12-01

    Full Text Available Foram avaliados em Cruz das Almas-BA, em dois ciclos, 69 clones Cavendish coletados em São Paulo, Minas Gerais, Bahia e Santa Catarina, sendo 49 do tipo Nanicão e 20 do tipo Grande Naine. Os clones foram estabelecidos em área experimental da Embrapa Mandioca e Fruticultura utilizando-se um delineamento em blocos ao acaso com cinco repetições e cinco plantas por parcela. Foram utilizados a irrigação por microaspersão, o espaçamento de 3,0 m x 2,0 m e um sistema de condução com três plantas/touceira. Os requerimentos agronômicos foram aplicados conforme preconizados para o cultivo. Na avaliação dos clones, foram consideradas as seguintes variáveis: altura da planta em centímetros (AP; número de dias do plantio à colheita (ND; peso do cacho em quilogramas (PC; número de frutos por cacho (NF; e comprimento do fruto em centímetros (CF. Pelos resultados obtidos, verificou-se uma variabilidade média em todas as variáveis avaliadas. Por apresentarem caracteres superiores, foram selecionados cinco clones de Grande Naine (G.N. Taperão, G.N.Rossete, G.N. Willians, G.N. Magário, G.N. SC-074 e quatro de Nanicão (N. IAC Abóbada Verde, N. Rossete, N. SC-0008 e N. SC-063.Sixty-nine clones of Cavendish type bananas (49 Nanicão and 20 Grande Naine collected in the States of São Paulo, Minas Gerais, Bahia and Santa Catarina were evaluated. The clones were established in an experimental area at the Cassava and Fruit Crops Research Center - EMBRAPA, using micro-aspersion irrigation, in plants spaced at 3.0 m x 2.0 m, and conducted with three plants/bushes. Random blocks were used with five replications and five plants per plot. All cultural requirements for the crop were carried out. For the clone evaluations, the following variables were considered: plant height in centimeters (PH; number of days from planting to yield (ND; weight of bunch in kilograms (WB; number of fruits per bunch (FB and length of fruit in centimeters (LF. The

  11. Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants.

    Science.gov (United States)

    Kamdem, Irénée; Hiligsmann, Serge; Vanderghem, Caroline; Bilik, Igor; Paquot, Michel; Thonart, Philippe

    2013-12-01

    We studied banana lignocellulosic biomass (BALICEBIOM) that is abandoned after fruit harvesting, and assessed its biochemical methane potential, because of its potential as an energy source. We monitored biogas production from six morphological parts (MPs) of the "Williams Cavendish" banana cultivar using a modified operating procedure (KOP) using KOH. Volatile fatty acid (VFA) production was measured using high performance liquid chromatography. The bulbs, leaf sheaths, petioles-midribs, leaf blades, rachis stems, and floral stalks gave total biogas production of 256, 205, 198, 126, 253, and 221 ml g⁻¹ dry matter, respectively, and total biomethane production of 150, 141, 127, 98, 162, and 144 ml g⁻¹, respectively. The biogas production rates and yields depended on the biochemical composition of the BALICEBIOM and the ability of anaerobic microbes to access fermentable substrates. There were no significant differences between the biogas analysis results produced using KOP and gas chromatography. Acetate was the major VFA in all the MP sample culture media. The bioconversion yields for each MP were below 50 %, showing that these substrates were not fully biodegraded after 188 days. The estimated electricity that could be produced from biogas combustion after fermenting all of the BALICEBIOM produced annually by the Cameroon Development Corporation-Del Monte plantations for 188 days is approximately 10.5 × 10⁶ kW h (which would be worth 0.80-1.58 million euros in the current market). This bioenergy could serve the requirements of about 42,000 people in the region, although CH₄ productivity could be improved.

  12. Crescimento de mudas micropropagadas da bananeira cv. Nanicão, em diferentes substratos e fontes de fertilizante = Growth of micropropagated cavendish banana seedlings in different substrates and fertilizer sources

    Directory of Open Access Journals (Sweden)

    Edson Shigueaki Nomura

    2008-07-01

    Full Text Available Com objetivo de avaliar o efeito de diferentes substratos combinados a fontes de fertilizantes no crescimento de mudas de bananeira micropropagadas cv. Nanicão (Musa spp. AAA, foi instalado um experimento em blocos casualizados, no esquema fatorial 5 x 3,com quatro repetições. Os substratos utilizados foram: S1 - Terra de subsolo + casca de arroz carbonizada + Rendmax Floreira®; S2 - Terra de subsolo + casca de arroz carbonizada + Organifol®; S3 - Terra de subsolo + casca de arroz carbonizada + Organifol® 9% SiO; S4 - Technes Vivatto®; S5 - Areia grossa + casca de arroz carbonizada+ Rendmax Floreira®. As fontes de fertilizantes foram: SA - sem adubo; LL - adubo de liberação lenta - Osmocote® 3M 14-14-14 (5,0 kg m-3 misturado ao substrato; e LN - adubo de liberação normal, 14-14-14 (7,5 g muda-1 aplicado em cobertura. Foram determinados a altura, o diâmetro do colo, o número de folhas, a área foliar e a matéria seca das plantas. Com base nas diferenças de crescimento, recomenda-se a utilização dos substratos S1, S2, S3 e S4, com adição de fertilizante 14-14-14, com liberação lenta (5,0 kg m-3 ou normal (7,5 g muda-1 de nutrientes.With the objective of evaluating the effect of different substrates combined with fertilizers in the growth of micropropagated seedlings of Cavendish banana (Musa spp. AAA, an experiment was conducted in a randomized block design, in a 5 x 3 factorial scheme, with four repetitions. The substrates used were: S1 – subsoil land + carbonized rice hull + Rendmax Floreira®; S2 - subsoil land + carbonizedrice hull + Organifol®; S3 - subsoil land + carbonized rice hull + Organifol® 9% SiO; S4 - Technes Vivatto®; S5 - thick sand + carbonized rice hull + Rendmax Floreira®. The fertilizer sources were: SA – without fertilizer; LL – slow-release fertilizer - Osmocote® 3M 14-14-14 (5.0 kg m-3 mixed in the substrate; and LN – normal-release fertilizer, 14-14-14 (7.5 g seedling-1 applied in

  13. In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.

    Science.gov (United States)

    Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert

    2014-02-01

    An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) α-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana.

  14. Crescimento de mudas micropropagadas da bananeira cv. Nanicão, em diferentes substratos e fontes de fertilizante = Growth of micropropagated cavendish banana seedlings in different substrates and fertilizer sources

    OpenAIRE

    Edson Shigueaki Nomura; Juliana Domingues Lima; Valéria Augusta Garcia; Domingos Sávio Rodrigues

    2008-01-01

    Com objetivo de avaliar o efeito de diferentes substratos combinados a fontes de fertilizantes no crescimento de mudas de bananeira micropropagadas cv. Nanicão (Musa spp. AAA), foi instalado um experimento em blocos casualizados, no esquema fatorial 5 x 3,com quatro repetições. Os substratos utilizados foram: S1 - Terra de subsolo + casca de arroz carbonizada + Rendmax Floreira®; S2 - Terra de subsolo + casca de arroz carbonizada + Organifol®; S3 - Terra de subsolo + casca de arroz carbonizad...

  15. Cavendish, Henry (1731-1810)

    Science.gov (United States)

    Murdin, P.

    2000-11-01

    English chemist and physicist. Cavendish used a sensitive torsion balance (the Cavendish balance), which was made to oscillate under the gravitational force of attraction of large masses, to measure the value of the gravitational constant, G, and used his value to determine the mass of the Earth....

  16. Nuevo método para la selección rapida de plantas de banano (Musa spp. AAA cv. Grande naine) transformadas con gen bar procedentes de campo, empleando glufosinato de amonio

    OpenAIRE

    Rafael Gómez-Kosky; Maritza Reyes Vega; Idalmis Bermúdez Caraballoso; Borys Chong Pérez; Yelenys Alvarado Capo

    2010-01-01

    Título en inglés: New method for rapid selection of banana (Musa spp. AAA cv. Grande naine) plants with bar gene in the field using ammonium gluphosinate Resumen Los genes marcadores de la selección son ampliamente utilizados para la transformación eficiente de diferentes cultivos, sin embargo, existen muy pocas referencias sobre el desarrollo de métodos de selección temprana en campo de plantas supuestamente transformadas con marcador de selección tipo herbicidas. La presente invest...

  17. MULTIPLICACIÓN DE HONGOS MICORRIZA ARBUSCULAR (H.M.A Y EFECTO DE LA MICORRIZACIÓN EN PLANTAS MICROPROPAGADAS DE BANANO (Musa AAA cv. Gran Enano (Musaceae MULTIPLICATION OF ARBUSCULAR MYCORRHIZAE FUNGI (AMF AND MYCORRHIZATION EFFECT IN MICROPROPAGATED PLANTS OF BANANA (Musa AAA cv. ‘Gran Enano’ (Musaceae

    Directory of Open Access Journals (Sweden)

    Carmen Elena Usuga Osorio

    2008-06-01

    Full Text Available Se evaluó el proceso de multiplicación de hongos que forman micorriza arbuscular (HMA, para lo cual se usaron diferentes tipos de inóculos entre ellos nativos de agroecosistemas bananeros del Urabá (Antioquia-Colombia, en sustrato sólido, con diferentes plantas hospedadoras y la infectividad y efectividad sobre plantas de banano (Musa AAA cv. Gran Enano. La colonización micorrizal promedio general de los HMA a las plantas trampa fue de 37,76 ± 21,86 %, con respecto a este porcentaje, las plantas B (Brachiaria decumbens y S (Sorgum vulgare fueron las que más favorecieron la simbiosis. Teniendo en cuenta el sustrato, el S2 (Arena 50 - suelo 50 y el S6 (Vermiculita 50-suelo 50 permitieron expresiones significativamente mayores respecto a los demás. El Sorgum vulgare y Pueraria phaseoloides y en el sustrato S1 (Arena 30 - suelo 70, se encontró un mayor número de esporas. La combinación planta-sustrato que más favoreció la asociación fue la planta trampa B en los sustratos S2 y S4 (cascarilla de arroz 50-suelo50 y la producción de esporas fueron las plantas K y S en el sustrato S1. La asociación micorrícica general en plantas de banano provenientes de cultivo de tejidos fue de 48,74 ± 30,44. No se encontraron diferencias significativas (P > 0,05 entre plantas de cero días con plantas de 30 de aclimatadas. Los inóculos que significativamente favorecieron la asociación fueron los provenientes de agroecosistemas bananeros al compararse con el inóculo comercial y el proveniente de ecosistemas naturales del Urabá. El mayor peso seco foliar y radical se encontró en plántulas de banano inoculadas con I5 (Inóculo proveniente de agroecosistema bananeros de la zona de estudio. Para las variables de crecimiento no se encontraron diferencias.The process of multiplication of arbuscular mycorrhizae fungi (AMF from indigenous banana agro-environments from Urabá (Antioquia - Colombia was evaluated, using solid substrate, with different

  18. Influencia de la pérdida foliar sobre la cosecha en el cv. Gruesa, Musa acuminata Colla (AAA, cultivado bajo invernadero en las Islas Canarias

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    Juan Cabrera Cabrera

    2010-12-01

    Full Text Available El cultivo de la variedad de platanera Gruesa, selección local de Dwarf Cavendish, ha experimentado un importante aumento en los últimos años en las Islas Canarias, tanto al aire libre como bajo invernadero. La eliminación de hojas, tras la floración, es una práctica habitual en los cultivos bajo invernadero. Asimismo es frecuente la pérdida de hojas por el efecto de los vientos en los cultivos al aire libre. El objetivo de este trabajo es evaluar, mediante simulación de pérdida foliar por daños mecánicos, la influencia que tiene la disminución de superficie foliar sobre el llenado y cosecha de la fruta en dicho cultivar. Para ello, cuatro meses antes de la cosecha se efectuaron cinco niveles de defoliación: 0%, 25%, 50%, 75% y 100%. Se valoran dos métodos diferentes de defoliación, eliminación de limbo foliar y tronchado de hojas con posterior corte de éstas. Se analizan y presentan datos morfológicos, fenológicos y productivos, así como valoración de la metodología empleada en este trabajo para la simulación de daños. A partir de un 25% de defoliado, equivalente a 7.5 hojas funcionales por planta, se detectaron diferencias significativas con las plantas testigos.

  19. A simple Cavendish experimental apparatus

    Science.gov (United States)

    Kossler, W. J.; Klein, Susann; Morrow, Dominick; Juliao, Andre

    2016-03-01

    A simple Cavendish apparatus is described that allows measurement of the gravitational constant G and makes observable the gravitational attraction between commonplace objects. The apparatus consists of a torsion balance constructed from readily available materials, including lead bricks and fishing weights ("sinkers"). A computer program is used to determine the gravitational field at the location of the small mass due to a nearby lead brick, which allows students to gain experience with numerical methods. Experimental results obtained are compatible with the accepted value of G.

  20. Nuevo método para la selección rapida de plantas de banano (Musa spp. AAA cv. Grande naine transformadas con gen bar procedentes de campo, empleando glufosinato de amonio

    Directory of Open Access Journals (Sweden)

    Rafael Gómez-Kosky

    2010-10-01

    Full Text Available Título en inglés: New method for rapid selection of banana (Musa spp. AAA cv. Grande naine plants with bar gene in the field using ammonium gluphosinate Resumen Los genes marcadores de la selección son ampliamente utilizados para la transformación eficiente de diferentes cultivos, sin embargo, existen muy pocas referencias sobre el desarrollo de métodos de selección temprana en campo de plantas supuestamente transformadas con marcador de selección tipo herbicidas. La presente investigación tuvo como objetivo evaluar el efecto del glufosinato de amonio a partir del herbicida Finale en los fragmentos de hojas de plantas de campo cultivados in vitro, para la selección de líneas transformadas de banano cv. Grande naine (Musa spp. AAA, con una construcción que porta como marcador de selección el gen bar. A partir del método desarrollado con el empleo de fragmentos de hojas de plantas procedentes de campo, cultivados in vitro en el medio de cultivo agar al 1% más agua y 30,0 g/l-1 de glufosinato de amonio se logró diferenciar las dos líneas transformadas del control no transformado, a partir de la expresión del gen bar. Ambas líneas fueron positivas en el análisis molecular de PCR. Estos resultados permiten disponer de una herramienta útil y simple como parte de un protocolo de transformación genética para el cultivo del banano, siendo el primer informe de este resultado a nivel internacional. Palabras clave: Musa; transformación genética; gen bar; glufosinato de amonio. Abstract Selector marker genes are widely used for the efficient transformation of different crops; however, there are very few references on the development of early selection methods in the fields of potentially transformed plants for herbicides. The objective of this research was to evaluate the effect of ammonium gluphosinate from the herbicide Finale in leaf fragments from in vitro plants grown in the fields for the selection of putative transformed

  1. Influencia de la pérdida foliar sobre la cosecha en el cv. Gruesa, Musa acuminata Colla (AAA, cultivado bajo invernadero en las Islas Canarias Influence of leaf removal in yield of cv. Gruesa, Musa acuminata Colla (AAA, cultivated under greenhouse in the Canary Islands

    Directory of Open Access Journals (Sweden)

    Juan Cabrera Cabrera

    2010-12-01

    Full Text Available El cultivo de la variedad de platanera Gruesa, selección local de Dwarf Cavendish, ha experimentado un importante aumento en los últimos años en las Islas Canarias, tanto al aire libre como bajo invernadero. La eliminación de hojas, tras la floración, es una práctica habitual en los cultivos bajo invernadero. Asimismo es frecuente la pérdida de hojas por el efecto de los vientos en los cultivos al aire libre. El objetivo de este trabajo es evaluar, mediante simulación de pérdida foliar por daños mecánicos, la influencia que tiene la disminución de superficie foliar sobre el llenado y cosecha de la fruta en dicho cultivar. Para ello, cuatro meses antes de la cosecha se efectuaron cinco niveles de defoliación: 0%, 25%, 50%, 75% y 100%. Se valoran dos métodos diferentes de defoliación, eliminación de limbo foliar y tronchado de hojas con posterior corte de éstas. Se analizan y presentan datos morfológicos, fenológicos y productivos, así como valoración de la metodología empleada en este trabajo para la simulación de daños. A partir de un 25% de defoliado, equivalente a 7.5 hojas funcionales por planta, se detectaron diferencias significativas con las plantas testigos.The banana cultivar Gruesa, a local Dwarf Cavendish selection, is increasingly planted in the Canary Islands, both in greenhouse and in the open air. Both methods present some degree of leaf loss throughout the crop cycle: the leaves of greenhouse plants are traditionally cut back after flowering, and open air plantations experience frequent wind damage. A trial was set up four months prior to harvest to evaluate fruit filling rate and yield of Gruesa subjected to five levels of defoliation (0%, 25%, 50%, 75%, and 100% loss of leaf area. Two defoliation techniques were used to simulate damage: removal of the midrib, and slashing the leaf blade followed later by complete removal. Morphological, phenological and production data are presented.

  2. Efeito da interação entre carvão ativado e N6-benzilaminopurina na propagação in vitro de bananeira, cv. Grand Naine (AAA Interaction effect between activated charcoal and N6-benzylaminopurine in the in vitro propagation of banana, cultivar Grand Naine (AAA

    Directory of Open Access Journals (Sweden)

    Frederico Henrique da Silva Costa

    2006-08-01

    Full Text Available O carvão ativado possui a propriedade de adsorver os compostos fenólicos liberados pela oxidação dos tecidos lesionados durante o cultivo in vitro. O objetivo deste trabalho foi avaliar os efeitos da interação entre o carvão ativado e diferentes concentrações de N6-benzilaminopurina (BAP na multiplicação in vitro da bananeira, cv. Grande Naine (AAA. O meio de cultura utilizado foi o MS, solidificado com 5 g.L-1 de ágar. O cultivo foi mantido em sala de crescimento a 25±2ºC, fotoperíodo de 16 horas e intensidade luminosa de 30 mmol.m-2s-1. Foram avaliadas a presença e a ausência de carvão ativado (0 e 3 g.L-1 e quatro concentrações de BAP (0; 2; 4 e 6 mg.L-1 no meio de cultura. O delineamento foi inteiramente casualizado, com cinco repetições, em um sistema fatorial 2x4. Os explantes foram avaliados a cada 30 dias, por um período de quatro subcultivos. Após cada subcultivo, o comprimento de brotações, a taxa de multiplicação, o vigor, o nível de oxidação das brotações emitidas e o número de raízes formadas foram avaliados. Independentemente das concentrações de BAP, o carvão ativado influenciou significativamente em todas as variáveis analisadas. De maneira geral, a adição de carvão ativado afetou negativamente a taxa de multiplicação, embora tenha melhorado o vigor e o número de raízes e diminuído a oxidação dos explantes. Na ausência de carvão ativado, o BAP proporcionou as maiores taxas de multiplicação das brotações.Active charcoal has the capacity to fix phenolic compounds released by the oxidation of manipulated tissues during in vitro cultivation. The present work objective was to evaluate the interaction effects between active charcoal, combined to different BAP concentrations in the in vitro banana propagation, cv. Grand Nain (AAA. The culture medium used was the MS, solidified with 5 g.L-1 agar. Cultures were kept in a growth room at 25 ± 2ºC with a photoperiod of 16 h at 30 mmol

  3. ABDOMINAL AORTIC ANEURYSM (AAA

    Directory of Open Access Journals (Sweden)

    Rajesh G

    2011-11-01

    Full Text Available AAA is defined as a distension of the infrarenal aorta by more than 50% (or 1.5 times compared with a corresponding healthy, aged and gender matched population. AAA afflicts 1 to 6 % of the general population aged more than 60 years and the incidence rises by approximately 0.15% annually. When the definition of a maximum external diameter ≥3 cm is used, the prevalence of AAA is upto 6 times greater in men than in women. AAAs are much more common than thoracic aortic aneurysms. Most common cause of AAA is atherosclerosis (95%. Less common causes include infectious or inflammatory origin or those associated with connective tissue disorders. Process of AAA formation is multifactorial. Other than the general risk factors for atherosclerosis, genetic predispo s iti on, aut o immunity and hemodynamic factors all play roles in its formation. AAA is 1.5 times more frequent in hypert ensive patients. Smokers have 8 times risk for developing AAA. The existence of familial aggre gation of AAA implicates genetic factors in the etiology of AAA. Women with AAA are more likely to have a positive family history of this disorder. Those with a family history of AAA have an increased risk of 30%, and their aneurysms tend to occur at a younger age and carry a greater risk of rupture than do sporadic aneurysms.

  4. Avaliação de clones de banana Cavendish Evaluation of cavendish banana clones

    OpenAIRE

    Sebastião de Oliveira E Silva; Elias Teixeira Pires; Rosa Karla Nogueira Pestana; Juliana da Silva Alves; Dreid de Cerqueira Silveira

    2006-01-01

    Na bananeira ocorrem variações somaclonais em taxa muito superior ao que se observa na maioria das outras culturas, provavelmente em função da instabilidade mitótica. Objetivou-se com o presente trabalho avaliar clones de bananeira Cavendish coletados em diferentes locais. Os clones Grande Naine (G.N. Taperão, G.N. Rossete, G.N. Williams, G.N. Magário, G.N. SC-074) e Nanicão (N. IAC Abóbora Verde, N. Rossete, N. SC-0008 e N. SC-063) coletados em São Paulo, Santa Catarina e Bahia foram avaliad...

  5. Utilización del método de superficie de respuesta para formular una base de banano (Musa AAA para batidos

    Directory of Open Access Journals (Sweden)

    Marta Gamboa White

    2010-06-01

    Full Text Available El Método de Superficie de Respuesta se utiliza para optimizar o reformular productos. Se usa principalmente para economizar dinero y reducir el tiempo de pruebas, al disminuir el número de ensayos. Se presenta la aplicación del Método de Superficie de Respuesta para desarrollar una base a partir de bananos (Musa AAA var. Cavendish cv. Gran Enano de rechazo de exportación a manera de caso para estudiar su uso. Por medio de una encuesta se identificó que el dulzor, espumosidad y sabor fueron los atributos que los consumidores consideraron más importantes en un batido. Con base en estos atributos y con los ingredientes: pulpa de banano, goma guar y azúcar, se elaboraron y seleccionaron varias mezclas base, que fueron degustadas, en forma de batidos en leche, por un grupo de 90 consumidores. Por medio del diseño de superficie de respuesta se determinó que el batido con la mayor aceptación estuvo constituido por un 77,0 % de leche y un 23,0 % de mezcla base, compuesta esta última por 0,12 % de goma guar, 80,50 % de pulpa de banano y un 19,50 % de azúcar. Un Análisis de Componentes Principales permitió determinar que el contenido de sacarosa, que define el grado de dulzor, fue el atributo más importante para la aceptación del batido y se demostró que la combinación del Método de Superficie de Respuesta con el Análisis de Componentes Principales constituyó una herramienta útil en la formulación y optimización de productos, sobre todo para entender la interrelación de las variables.

  6. Maxwell's enduring legacy a scientific history of the Cavendish laboratory

    CERN Document Server

    Longair, Malcolm

    2016-01-01

    The Cavendish Laboratory is arguably the most famous physics laboratory in the world. Founded in 1874, it rapidly gained a leading international reputation through the researches of the Cavendish professors beginning with Maxwell, Rayleigh, J. J. Thomson, Rutherford and Bragg. Its name will always be associated with the discoveries of the electron, the neutron, the structure of the DNA molecule and pulsars, but these are simply the tip of the iceberg of outstanding science. The physics carried out in the laboratory is the central theme of the book and this is explained in reasonably non-technical terms. The research activities are set in their international context. Generously illustrated, with many pictures of the apparatus used and diagrams from the original papers, the story is brought right up to date with descriptions of the science carried out under the leadership of the very different personalities of Mott, Pippard and Edwards.

  7. The LHC at the AAAS

    CERN Multimedia

    CERN Bulletin

    2011-01-01

    The American Association for the Advancement of Science held its annual meeting in the Walter E. Washington Convention Center in Washington D.C. last week.   Veteran science writer Tim Radford introduces LHC scientists during a media briefing at the AAAS annual meeting. Left to right: Felicitas Pauss, Tom LeCompte, Yves Schutz and Nick Hadley. As the world’s largest popular science meeting, the AAAS meeting is a major event in the calendar of science journalists.  At this year’s LHC session, CERN’s coordinator for international relations, Felicitas Pauss, opened the discussion, paving the way for Tom LeCompte of ATLAS, Joe Incandela of CMS, Yves Schutz of ALICE and Monica Pepe-Altarelli of LHCb to report on the status of the first year’s analysis from their experiments.    

  8. Suppression of Type-II Diabetes with Dyslipidemia and Nephropathy by Peels of Musa cavendish Fruit.

    Science.gov (United States)

    Navghare, Vijay; Dhawale, Shashikant

    2016-10-01

    Musa cavendish, peels has local and traditional use to promote wound healing, hyperglycemia, ulceration etc. The present work investigated the lipid lowering; nephroprotective and glucose lowering properties of ethanolic extract of peels of Musa cavendish (EMC) in alloxan-induced diabetic rats. The EMC 250, 500 and 1000 mg/kg/day and the vehicle were administered orally to alloxan-induced diabetic rats (n = 6) for 3 weeks. Changes in plasma glucose, lipid profile along with kidney function before and after treatment with EMC were recorded. The ethanolic extract of peels of Musa cavendish reduced blood glucose, serum triglyceride, cholesterol, LDL cholesterol and creatinine levels and improvement in body weight, liver glycogen, serum HDL cholesterol, serum albumin and total protein level when compared with untreated rats. Musa cavendish has lipid lowering, nephroprotective and antidiabetic property by regulating glucose uptake in the liver and muscles by restoring the intracellular energy balance. PMID:27605735

  9. BABA诱导香蕉果实抗病性与贮藏期活性氧积累的关系%Accumulation of Reactive Oxygen Species Related to Disease Resistance Induced by BABA in Postharvest Banana (Musa AAA. cv. Brazil) Fruit

    Institute of Scientific and Technical Information of China (English)

    谭卫萍; 庞学群; 张昭其; 黄雪梅

    2014-01-01

    果实的抗病性。%[Objective]Effects of β-aminobutyric acid (BABA) on diseases-tolerance of harvested banana (Musa AAA Group cv. Brazil) and the possible mechanism were investigated to provide theoretical basis for the new technology of resistant and preservation.[Method] In this study, diphenylene iodonium (DPI), a NADPH oxidase specific inhibitor, was used to inhibit ROS generation in BABA treated fruits. After 5 g·L-1 BABA treatment or 3.14 mg·L-1DPI followed with 5 g·L-1BABA treatment, banana fruits were inoculated withCollectotrichum musae (2× 105 spores/mL) at 0, 3, 6, 12, 24, 48, and 72 h, respectively, and stored at20±2℃ and RH 85%-95%. The disease spot sizes were determined at 5 d to 16 d. The fruit inoculated withC. musae at 24 h after BABA or DPI treatments and stored at (20±2)℃ and RH85%-95%, and the peel was used for determination of production rate, activities of catalase (CAT) and ascorbate peroxidase (APX), β-1, 3-glucanase(GUN), phenylalanine ammonia lyase(PAL), chitinase (CHI), gene expressions ofMaCAT,MaAPX,MaGLU, MaCHI,MaPAL1andMaNOX during storage.[Result] BABA treatment effectively reduced the disease spot sizes on the peel of inoculated banana at 24 h after BABA treatment and cultured for 5 d, showing that BABA treatment needed proper time to have an effect. The production rate andMaNOX gene expression of BABA treated fruits were evidently higher than those of control during 5-12 d storage. Compared with the control, CAT activity andMaCAT expression were higher on day 5 and 1-5 d, respectively. APX activity andMaAPX expression were higher on 5-8 d and 14 d, and 1-5 d, respectively. CHI activity andMaCHI expression was higher on 5-12 d and 1-5 d, respectively. Both of GUN activity and MaGLU expression were higher on 8-14 d. Both PAL activity andMaPAL1 gene expression were higher on 12-14 d . No significant difference was found in other time points. DPI combined with BABA treatment suppressed the above effects of BABA treatment.[Conclusion]These results strongly suggest

  10. Leadership and creativity a history of the Cavendish Laboratory, 1871–1919

    CERN Document Server

    Kim, Dong-Won

    2002-01-01

    Historical accounts of successful laboratories often consist primarily of reminiscences by their directors and the eminent people who studied or worked in these laboratories. Such recollections customarily are delivered at the celebration of a milestone in the history of the laboratory, such as the institution's fiftieth or one­ hundredth anniversary. Three such accounts of the Cavendish Laboratory at the University of Cambridge have been recorded. The first of these, A History of the Cavendish Laboratory, 1871-1910, was published in 1910 in honor of the twenty­ fifth anniversary of Joseph John Thomson's professorship there. The second, The Cavendish Laboratory, 1874-1974, was published in 1974 to commemorate the one­ hundredth anniversary of the Cavendish. The third, A Hundred Years and More of Cambridge Physics, is a short pamphlet, also published at the centennial of the 1 Cavendish. These accounts are filled with the names of great physicists (such as James Clerk Maxwell, Lord Rayleigh, J. J. Thomson, ...

  11. Abraham Pais Prize for History of Physics Talk: Henry Cavendish, John Michell, Weighing the Stars

    Science.gov (United States)

    McCormmach, Russell

    2010-03-01

    This talk is about an interaction between two 18th-century natural philosophers (physical scientists), Henry Cavendish and John Michell, and its most important outcome, the experiment of weighing the world (their name for it) using a torsion balance (our name for it). Michell was the most inventive of the 18th century English natural philosophers, and Cavendish was the first of his countrymen to possess abilities at all comparable with Newton's. By their interests and skills, they were drawn to one another. Both were universal natural philosophers, equally adept at building scientific instruments, performing experiments, constructing theory, and using mathematics; both had a penchant for exacting, quantitative work. Both also had fitful habits of publication, which did not begin to reveal the range of their work, to the mystification of later scientists and historians. Late in life, Cavendish and Michell turned their attention to the force that Newton had examined most completely, a singular triumph of his natural philosophy, the force of universal gravitation. Over the course of the 18th century, abundant evidence of attraction had been gathered from the motions of the earth, moon, planets, and comets, phenomena which span the intermediate range of masses, sizes, and distances. But in three domains of experience, involving the extreme upper and lower limits of masses and dimensions, the universality of gravitation remained an article of faith. These were the gravity of the ``fixed'' stars, the mutual attraction of terrestrial bodies, and the gravitation of light and other special substances. Michell took on himself the task of deducing observable consequences from each of these prospective instances of universal gravitation. Cavendish encouraged Michell, and he followed up the resulting observational and experimental questions. The experiment of weighing the world was the last experiment Mitchell planned and the last experiment Cavendish published. The capstone of

  12. 26 CFR 1.1368-2 - Accumulated adjustments account (AAA).

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 11 2010-04-01 2010-04-01 true Accumulated adjustments account (AAA). 1.1368-2... adjustments account (AAA). (a) Accumulated adjustments account—(1) In general. The accumulated adjustments account is an account of the S corporation and is not apportioned among shareholders. The AAA is...

  13. AAA-DDD triple hydrogen bond complexes.

    Science.gov (United States)

    Blight, Barry A; Camara-Campos, Amaya; Djurdjevic, Smilja; Kaller, Martin; Leigh, David A; McMillan, Fiona M; McNab, Hamish; Slawin, Alexandra M Z

    2009-10-01

    Experiment and theory both suggest that the AAA-DDD pattern of hydrogen bond acceptors (A) and donors (D) is the arrangement of three contiguous hydrogen bonding centers that results in the strongest association between two species. Murray and Zimmerman prepared the first example of such a system (complex 3*2) and determined the lower limit of its association constant (K(a)) in CDCl(3) to be 10(5) M(-1) by (1)H NMR spectroscopy (Murray, T. J. and Zimmerman, S. C. J. Am. Chem. Soc. 1992, 114, 4010-4011). The first cationic AAA-DDD pair (3*4(+)) was described by Bell and Anslyn (Bell, D. A. and Anslyn, E. A. Tetrahedron 1995, 51, 7161-7172), with a K(a) > 5 x 10(5) M(-1) in CH(2)Cl(2) as determined by UV-vis spectroscopy. We were recently able to quantify the strength of a neutral AAA-DDD arrangement using a more chemically stable AAA-DDD system, 6*2, which has an association constant of 2 x 10(7) M(-1) in CH(2)Cl(2) (Djurdjevic, S., Leigh, D. A., McNab, H., Parsons, S., Teobaldi, G. and Zerbetto, F. J. Am. Chem. Soc. 2007, 129, 476-477). Here we report on further AA(A) and DDD partners, together with the first precise measurement of the association constant of a cationic AAA-DDD species. Complex 6*10(+)[B(3,5-(CF(3))(2)C(6)H(3))(4)(-)] has a K(a) = 3 x 10(10) M(-1) at RT in CH(2)Cl(2), by far the most strongly bound triple hydrogen bonded system measured to date. The X-ray crystal structure of 6*10(+) with a BPh(4)(-) counteranion shows a planar array of three short (NH...N distances 1.95-2.15 A), parallel (but staggered rather than strictly linear; N-H...N angles 165.4-168.8 degrees), primary hydrogen bonds. These are apparently reinforced, as theory predicts, by close electrostatic interactions (NH-*-N distances 2.78-3.29 A) between each proton and the acceptor atoms of the adjacent primary hydrogen bonds.

  14. Combination of membrane technologies for purification of L (+) - lactic acid from juice of banana (Musa AAA, variety Cavendish cultivar Gram naine) obtained from an agroindustrial waste

    International Nuclear Information System (INIS)

    The process that has allowed recovery and purification of the L (+)-acid present in the juice fermented waste produced from banana was developed, treated enzymatically, using tangential nanofiltration. The effect of the enzymatic treatment was evaluated on physical chemical parameters of fermented banana juice. The process parameters of centrifugal clarification and microfiltration were characterized on banana juice as activities prior operations to recovery and purification of lactic acid. The temperature and the transmembrane pressure on the permeate flow and the performance of recovery and purification of lactic acid were evaluated by the ultrafiltration and nanofiltration processes. The properties physico-chemical the banana juice fermented and of the liquid filtrate obtained at the stage recovery and purification of lactic acid were compared by ultrafiltration

  15. Regulatory Circuits of the AAA+ Disaggregase Hsp104

    OpenAIRE

    Franzmann, Titus M.; Czekalla, Anna; Walter, Stefan G.

    2011-01-01

    Yeast Hsp104 is an AAA+ chaperone that rescues proteins from the aggregated state. Six protomers associate to form the functional hexamer. Each protomer contains two AAA+ modules, NBD1 and NBD2. Hsp104 converts energy provided by ATP into mechanical force used to thread polypeptides through its axial channel, thereby disrupting protein aggregates. But how the action of its 12 AAA+ domains is co-ordinated to catalyze disaggregation remained unexplained. Here, we identify a sophisticated allost...

  16. No actual measurement ... was required: Maxwell and Cavendish's null method for the inverse square law of electrostatics

    CERN Document Server

    Falconer, Isobel

    2016-01-01

    In 1877 James Clerk Maxwell and his student Donald McAlister refined Henry Cavendish's 1773 null experiment demonstrating the absence of electric charge inside a charged conductor. Such absence of charge was a mathematical prediction of the inverse square law, and both Cavendish and Maxwell took the experiment as verifying the law. However, Maxwell had previously expressed absolute conviction in the law, based on results of Faraday's. So what was the value to Maxwell of replicating Cavendish's experiment? This paper examines the mathematical and instrumental contexts of Maxwell's experiment. It situates the experiment in an assessment of the status of the inverse square law in the 1870s based on textbooks, Maxwell's drive to develop both an electrical programme and a "doctrine of method", and an investigation of the understanding of null methods in the 1870s. It demonstrates that he had previously shifted the evidential context of Faraday's work to suit his aims. It concludes that the experiment served both a...

  17. Integrated Control of Axonemal Dynein AAA+ Motors

    Science.gov (United States)

    King, Stephen M.

    2012-01-01

    Axonemal dyneins are AAA+ enzymes that convert ATP hydrolysis to mechanical work. This leads to the sliding of doublet microtubules with respect to each other and ultimately the generation of ciliary/flagellar beating. However, in order for useful work to be generated, the action of individual dynein motors must be precisely controlled. In addition, cells modulate the motility of these organelles through a variety of second messenger systems and these signals too must be integrated by the dynein motors to yield an appropriate output. This review describes the current status of efforts to understand dynein control mechanisms and their connectivity focusing mainly on studies of the outer dynein arm from axonemes of the unicellular biflagellate green alga Chlamydomonas. PMID:22406539

  18. AAAS Communicating Science Program: Reflections on Evaluation

    Science.gov (United States)

    Braha, J.

    2015-12-01

    The AAAS Center for Public Engagement (Center) with science builds capacity for scientists to engage public audiences by fostering collaboration among natural or physical scientists, communication researchers, and public engagement practitioners. The recently launched Leshner Leadership Institute empowers cohorts of mid-career scientists to lead public engagement by supporting their networks of scientists, researchers, and practitioners. The Center works closely with social scientists whose research addresses science communication and public engagement with science to ensure that the Communicating Science training program builds on empirical evidence to inform best practices. Researchers ( Besley, Dudo, & Storkdieck 2015) have helped Center staff and an external evaluator develop pan instrument that measures progress towards goals that are suggested by the researcher, including internal efficacy (increasing scientists' communication skills and confidence in their ability to engage with the public) and external efficacy (scientists' confidence in engagement methods). Evaluation results from one year of the Communicating Science program suggest that the model of training yields positive results that support scientists in the area that should lead to greater engagement. This talk will explore the model for training, which provides a context for strategic communication, as well as the practical factors, such as time, access to public engagement practitioners, and technical skill, that seems to contribute to increased willingness to engage with public audiences. The evaluation program results suggest willingness by training participants to engage directly or to take preliminary steps towards engagement. In the evaluation results, 38% of trained scientists reported time as a barrier to engagement; 35% reported concern that engagement would distract from their work as a barrier. AAAS works to improve practitioner-researcher-scientist networks to overcome such barriers.

  19. First report offusarium oxysporumf. sp.cubensetropical race 4 causing panama disease in cavendish bananas in Pakistan and Lebanon

    NARCIS (Netherlands)

    Ordoñez, N.; García-Bastidas, F.; Laghari, H.B.; Akkary, M.Y.; Harfouche, E.N.; Awar, al B.N.; Kema, G.H.J.

    2016-01-01

    Panama disease of banana, caused byFusarium oxysporumf. sp.cubense(Foc), poses a great risk to global banana production. Tropical race 4 (TR4) of Foc, which affects Cavendish bananas as well as many other banana cultivars (Ploetz 2006), was confirmed for the first time outside Southeast Asia in Jord

  20. 准确速判‘巴西蕉’果实成熟度方法研究%Study on a Quick and Accurate Method to Forecast Maturity of Brazilian Cavendish Banana

    Institute of Scientific and Technical Information of China (English)

    王南南; 樊小林; 刘延涛; 刘庆虎

    2011-01-01

    Under two different kinds of nutrient management systems, a field experiment by taking tissue-cultured plant of Brazilian Cavendish banana (Musa AAA Giant Cavendish 'Brazil') as seedling was conducted to establish a fast and accurately quantitative method to forecast the fruit maturity by determination and comparison of finger length, girth and fresh weight as well as the ratio of peel to pulp of every hand per fruit bunch, and by observation the percentage of inflorescence emergence and harvested plants of every treatment at weekly intervals. The results showed that the finger length of banana increased progressively with time, and the same were true for the finger girth, weight and ratio of pulp to peel in fresh weight. The finger length and weight appeared to be variable among hands, but the finger girth stable, which all were significantly affected by nutrient management system and could not be used to forecast maturity of Brazilian Cavendish banana. The date of harvest was considerably influenced by the nutrient management system as well. However, there was no significant difference of the ratio of pulp to peel at harvest either among the 1" to 3rd hands or among the 4th to 6th hands under both nutrient management systems. Therefore, the ratio value of pulp to peel could be used as a quantitative index to judge the fruit maturity of Brazilian Cavendish banana. When the average ratio value reached either 1.65 for the 1st to 3rd hands or 1.54 for the 4th to 6th hands, the 75% fruit maturity was obtained.%以‘巴西蕉’组培苗为试材,于大田条件下,分别在反梳期、膨大前期、膨大后期、采收期测定2种供肥模式下果穗各梳的农艺性状(指长、指围、指重)和鲜果肉皮比,且自抽蕾后定期观测抽蕾率和收获率,试图探寻一种判断‘巴西蕉’果实成熟度之简便易行的定量方法.结果表明:随着果实生长,‘巴西蕉’指长、指围、指重和鲜果肉皮比不断增大;果梳之间

  1. Microbiological and physicochemical factors affecting Aspergillus section Flavi incidence in Cavendish banana (Musa cavendishii) chips production in Southern Philippines.

    Science.gov (United States)

    Sales, A C; Azanza, P V; Yoshizawa, T

    2005-01-01

    Microbiological and physicochemical factors affecting the incidence of Aspergillus section Flavi in dried Cavendish banana (Musa cavendishii) chips production in Southern Philippines were examined. The average counts of Aspergillus section Flavi (AFC) in fresh and dried Cavendish bananas from 10 production batches of the Philippine Agro-Industrial Development Cooperative in Davao del Norte, Southern Philippines were 1.2 x 10(2) and 1.6 x 10(2) cfu/g, respectively. Isolates from both samples were identified to be Aspergillus flavus based on spore type and conidial structure of isolates. An increasing trend in the AFC of Cavendish bananas was observed during dried banana chips processing. Variability in the AFC between production batches was attributed to differences in aerobic and fungal populations and physicochemical characteristics of the fruits, peel damage of the raw materials, concentration of AFC in the air and food-contact surfaces of the production area, and temperature and relative humidity (RH) conditions of the environment during production and storage. Physicochemical characteristics of Cavendish bananas from the receipt of raw materials up to the first day of drying were within the reported range of values allowing growth and toxin production by aflatoxigenic fungi. Air-borne AFC varied depending on the section of the production area examined. The close proximity of the waste disposal area from the production operation to the preparation, drying and storage areas suggests that cross-contamination, probably air-borne or insect-borne was a likely occurrence. The hands of workers were also identified as AFC sources. Results of this study highlight the need for the development of strategies to control aflatoxigenic fungi and aflatoxin contamination in Philippine dried Cavendish bananas.

  2. Emgu CV essentials

    CERN Document Server

    Shi, Shin

    2013-01-01

    This book provides a practical guide to Emgu CV libraries, with sample code and examples used throughout to explain the concepts clearly. Each chapter deals with a different aspect of the Computer Vision field and the implementation of that topic in Emgu CV.If you are a C# programmer working on computer vision projects, this book is for you. You should have prior experience with C#.

  3. Inhibitors of the AAA+ Chaperone p97

    Directory of Open Access Journals (Sweden)

    Eli Chapman

    2015-02-01

    Full Text Available It is remarkable that a pathway as ubiquitous as protein quality control can be targeted to treat cancer. Bortezomib, an inhibitor of the proteasome, was first approved by the US Food and Drug Administration (FDA more than 10 years ago to treat refractory myeloma and later extended to lymphoma. Its use has increased the survival rate of myeloma patients by as much as three years. This success was followed with the recent accelerated approval of the natural product derived proteasome inhibitor carfilzomib (Kyprolis®, which is used to treat patients with bortezomib-resistant multiple myeloma. The success of these two drugs has validated protein quality control as a viable target to fight select cancers, but begs the question why are proteasome inhibitors limited to lymphoma and myeloma? More recently, these limitations have encouraged the search for additional targets within the protein quality control system that might offer heightened cancer cell specificity, enhanced clinical utility, a lower rate of resistance, reduced toxicity, and mitigated side effects. One promising target is p97, an ATPase associated with various cellular activities (AAA+ chaperone. p97 figures prominently in protein quality control as well as serving a variety of other cellular functions associated with cancer. More than a decade ago, it was determined that up-regulation of p97 in many forms of cancer correlates with a poor clinical outcome. Since these initial discoveries, a mechanistic explanation for this observation has been partially illuminated, but details are lacking. Understandably, given this clinical correlation, myriad roles within the cell, and its importance in protein quality control, p97 has emerged as a potential therapeutic target. This review provides an overview of efforts towards the discovery of small molecule inhibitors of p97, offering a synopsis of efforts that parallel the excellent reviews that currently exist on p97 structure, function, and

  4. CV : [luuletused] / Mari Vallisoo

    Index Scriptorium Estoniae

    Vallisoo, Mari, 1950-2013

    2007-01-01

    Sisu: CV ; Sõnaraamat ; Tegijad ; "Kus leemekulp?..." ; "Veel üks viimnepäev mis asja teha sellega..." ; "Ülemise riiuli ääre pääl...", "Alkeemik Alberti elamus...", "Valge pulber veel enam valgega...", "Meie isa, kes sa...", "Ükskord, kui Maa...", "Kevadvesi ja -pori...", "Mõisnik Meeskurat müüs mu vaarisale..."

  5. The Adult Asperger Assessment (AAA): A Diagnostic Method

    Science.gov (United States)

    Baron-Cohen, Simon; Wheelwright, Sally; Robinson, Janine; Woodbury-Smith, Marc

    2005-01-01

    At the present time there are a large number of adults who have "suspected" Asperger syndrome (AS). In this paper we describe a new instrument, the Adult Asperger Assessment (AAA), developed in our clinic for adults with AS. The need for a new instrument relevant to the diagnosis of AS in adulthood arises because existing instruments are designed…

  6. AAA: Road Debris a Mounting Danger on U.S. Highways

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_160383.html AAA: Road Debris a Mounting Danger on U.S. Highways ... and more than 500 deaths, according to the AAA Foundation for Traffic Safety. Crashes involving vehicle-related ...

  7. Molecularly Defined Nanostructures Based on a Novel AAA-DDD Triple Hydrogen-Bonding Motif.

    Science.gov (United States)

    Papmeyer, Marcus; Vuilleumier, Clément A; Pavan, Giovanni M; Zhurov, Konstantin O; Severin, Kay

    2016-01-26

    A facile and flexible method for the synthesis of a new AAA-DDD triple hydrogen-bonding motif is described. Polytopic supramolecular building blocks with precisely oriented AAA and DDD groups are thus accessible in few steps. These building blocks were used for the assembly of large macrocycles featuring four AAA-DDD interactions and a macrobicyclic complex with a total of six AAA-DDD interactions.

  8. CV-990 LSRA

    Science.gov (United States)

    1992-01-01

    A NASA CV-990, modified as a Landing Systems Research Aircraft (LSRA), is serviced on the ramp at NASA's Dryden Flight Research Center, Edwards, California, before a test of the space shuttle landing gear system. The space shuttle landing gear test unit, operated by a high-pressure hydraulic system, allowed engineers to assess and document the performance of space shuttle main and nose landing gear systems, tires and wheel assemblies, plus braking and nose wheel steering performance. The series of 155 test missions for the space shuttle program provided extensive data about the life and endurance of the shuttle tire systems and helped raise the shuttle crosswind landing limits at Kennedy.

  9. Increased levels of thioredoxin in patients with abdominal aortic aneurysms (AAAs). A potential link of oxidative stress with AAA evolution

    DEFF Research Database (Denmark)

    Martinez-Pinna, R; Lindholt, Jes S.; Blanco-Colio, L M;

    2010-01-01

    Oxidative stress is a main mechanism involved in vascular pathologies. Increased thioredoxin (TRX) levels have been observed in several oxidative stress-associated cardiovascular diseases. We aim to test the potential role of TRX as a biomarker of oxidative stress in abdominal aortic aneurysm (AAA)....

  10. Charged particle therapy for cancer: The inheritance of the Cavendish scientists?

    Energy Technology Data Exchange (ETDEWEB)

    Jones, Bleddyn [Department of Clinical Oncology, Queen Elizabeth University Hospital, University Hospitals NHS Trust, Birmingham University, Edgbaston, Birmingham B15 2TH (United Kingdom)], E-mail: b.jones.1@bham.ac.uk; Dale, Roger G.; Carabe-Fernandez, Alejandro [Department of Radiation Physics and Radiobiology, Imperial College Healthcare NHS Trust, Charing Cross Hospital, London W6 8RF (United Kingdom)

    2009-03-15

    The history of developments in atomic physics and its applications follows the decisive input provided by Maxwell and subsequent discoveries by his successors at the Cavendish Laboratory. In medicine the potential applications of particle physics (with the notable exception of the electron) were unfortunately delayed by the disappointing experiences with neutron therapy, which produced long-term scepticism. Neutrons are not appropriate for cancer therapy because not only their physical dose distributions offer no advantages over X-rays, but also their biological dose distributions are worse. The much improved dose distributions achieved with charged particles offer real prospects for better treatment outcomes because of the large reduction in the volume of unnecessarily irradiated tissue in many situations. Charged particle therapy is relatively new and can be applied with increasing confidence due to advances in radiology and computing, but at present there are insufficient numbers of treatment facilities to produce statistically powerful studies to compare treatment outcomes with those of X-rays. Considerable progress has been achieved in Japan and Germany with pilot studies of carbon ions but their efficacy compared with protons needs to be tested: in theory carbon should be better for intrinsically radio-resistant and for the most hypoxic tumours. The optimisation of proton and ion beam therapy in clinical practice remains to be achieved, but there are good scientific reasons why these modalities will be preferred by patients and their physicians in the future. Regrettably, despite hosting many of the momentous discoveries that enabled the development of charged particle therapy, the UK is slow to adopt and implement this very important form of cancer treatment.

  11. Transcriptome profiling of resistant and susceptible Cavendish banana roots following inoculation with Fusarium oxysporum f. sp. cubense tropical race 4

    Directory of Open Access Journals (Sweden)

    Li Chun-yu

    2012-08-01

    Full Text Available Abstract Background Fusarium wilt, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4, is considered the most lethal disease of Cavendish bananas in the world. The disease can be managed in the field by planting resistant Cavendish plants generated by somaclonal variation. However, little information is available on the genetic basis of plant resistance to Foc TR4. To a better understand the defense response of resistant banana plants to the Fusarium wilt pathogen, the transcriptome profiles in roots of resistant and susceptible Cavendish banana challenged with Foc TR4 were compared. Results RNA-seq analysis generated more than 103 million 90-bp clean pair end (PE reads, which were assembled into 88,161 unigenes (mean size = 554 bp. Based on sequence similarity searches, 61,706 (69.99% genes were identified, among which 21,273 and 50,410 unigenes were assigned to gene ontology (GO categories and clusters of orthologous groups (COG, respectively. Searches in the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG mapped 33,243 (37.71% unigenes to 119 KEGG pathways. A total of 5,008 genes were assigned to plant-pathogen interactions, including disease defense and signal transduction. Digital gene expression (DGE analysis revealed large differences in the transcriptome profiles of the Foc TR4-resistant somaclonal variant and its susceptible wild-type. Expression patterns of genes involved in pathogen-associated molecular pattern (PAMP recognition, activation of effector-triggered immunity (ETI, ion influx, and biosynthesis of hormones as well as pathogenesis-related (PR genes, transcription factors, signaling/regulatory genes, cell wall modification genes and genes with other functions were analyzed and compared. The results indicated that basal defense mechanisms are involved in the recognition of PAMPs, and that high levels of defense-related transcripts may contribute to Foc TR4 resistance in

  12. Bears in Eden, or, this is not the garden you're looking for: Margaret Cavendish, Robert Hooke and the limits of natural philosophy.

    Science.gov (United States)

    Lawson, Ian

    2015-12-01

    This paper investigates Margaret Cavendish's characterization of experimental philosophers as hybrids of bears and men in her 1666 story The Description of a New World, Called the Blazing World. By associating experimental philosophers, in particular Robert Hooke and his microscope, with animals familiar to her readers from the sport of bear-baiting, Cavendish constructed an identity for the fellows of the Royal Society of London quite unlike that which they imagined for themselves. Recent scholarship has illustrated well how Cavendish's opposition to experimental philosophy is linked to her different natural-philosophical, political and anthropological ideas. My contribution to this literature is to examine the meanings both of bears in early modern England and of microscopes in experimental rhetoric, in order to illustrate the connection that Cavendish implies between the two. She parodied Hooke's idea that his microscope extended his limited human senses, and mocked his aim that by so doing he could produce useful knowledge. The bear-men reflect inhuman ambition and provide a caution against ignoring both the order of English society and the place of humans in nature.

  13. Phenylalanine and tyrosine levels are rate-limiting factors in production of health promoting metabolites in Vitis vinifera cv. Gamay Red cell suspension.

    Science.gov (United States)

    Manela, Neta; Oliva, Moran; Ovadia, Rinat; Sikron-Persi, Noga; Ayenew, Biruk; Fait, Aaron; Galili, Gad; Perl, Avichai; Weiss, David; Oren-Shamir, Michal

    2015-01-01

    Environmental stresses such as high light intensity and temperature cause induction of the shikimate pathway, aromatic amino acids (AAA) pathways, and of pathways downstream from AAAs. The induction leads to production of specialized metabolites that protect the cells from oxidative damage. The regulation of the diverse AAA derived pathways is still not well understood. To gain insight on that regulation, we increased AAA production in red grape Vitis vinifera cv. Gamay Red cell suspension, without inducing external stress on the cells, and characterized the metabolic effect of this induction. Increased AAA production was achieved by expressing a feedback-insensitive bacterial form of 3-deoxy- D-arabino-heptulosonate 7-phosphate synthase enzyme (AroG (*)) of the shikimate pathway under a constitutive promoter. The presence of AroG(*) protein led to elevated levels of primary metabolites in the shikimate and AAA pathways including phenylalanine and tyrosine, and to a dramatic increase in phenylpropanoids. The AroG (*) transformed lines accumulated up to 20 and 150 fold higher levels of resveratrol and dihydroquercetin, respectively. Quercetin, formed from dihydroquercetin, and resveratrol, are health promoting metabolites that are induced due to environmental stresses. Testing the expression level of key genes along the stilbenoids, benzenoids, and phenylpropanoid pathways showed that transcription was not affected by AroG (*). This suggests that concentrations of AAAs, and of phenylalanine in particular, are rate-limiting in production of these metabolites. In contrast, increased phenylalanine production did not lead to elevated concentrations of anthocyanins, even though they are also phenylpropanoid metabolites. This suggests a control mechanism of this pathway that is independent of AAA concentration. Interestingly, total anthocyanin concentrations were slightly lower in AroG(*) cells, and the relative frequencies of the different anthocyanins changed as well.

  14. Phenylalanine and tyrosine levels are rate-limiting factors in production of health promoting metabolites in Vitis vinifera cv. Gamay Red cell suspension

    Directory of Open Access Journals (Sweden)

    Neta eManela

    2015-07-01

    Full Text Available Environmental stresses such as high light intensity and temperature cause induction of the shikimate pathway, aromatic amino acids (AAA pathways, and of pathways downstream from AAAs. The induction leads to production of specialized metabolites that protect the cells from oxidative damage. The regulation of the diverse AAA derived pathways is still not well understood. To gain insight on that regulation, we increased AAA production in red grape Vitis vinifera cv. Gamay Red cell suspension, without inducing external stress on the cells, and characterized the metabolic effect of this induction. Increased AAA production was achieved by expressing a feedback-insensitive bacterial form of 3-deoxy- D-arabino-heptulosonate 7-phosphate synthase enzyme (AroG* of the shikimate pathway under a constitutive promoter. The presence of AroG* protein led to elevated levels of primary metabolites in the shikimate and AAA pathways including phenylalanine and tyrosine, and to a dramatic increase in phenylpropanoids. The AroG* transformed lines accumulated up to 20 and 150 fold higher levels of resveratrol and dihydroquercetin, respectively. Quercetin, formed from dihydroquercetin, and resveratrol, are health promoting metabolites that are induced due to environmental stresses. Testing the expression level of key genes along the stilbenoids, benzenoids and phenylpropanoid pathways showed that transcription was not affected by AroG*. This suggests that concentrations of AAAs, and of phenylalanine in particular, are rate-limiting in production of these metabolites. In contrast, increased phenylalanine production did not lead to elevated concentrations of anthocyanins, even though they are also phenylpropanoid metabolites. This suggests a control mechanism of this pathway that is independent of AAA concentration. Interestingly, total anthocyanin concentrations were slightly lower in AroG* cells, and the relative frequencies of the different anthocyanins changed as

  15. Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain).

    Science.gov (United States)

    Chaurasia, Akhilesh Kumar; Patil, Hemant Bhagwan; Azeez, Abdul; Subramaniam, Vadakanthara Ramakrishnan; Krishna, Bal; Sane, Aniruddha Prafullachandra; Sane, Prafullachandra Vishnu

    2016-01-01

    The CONSTANS (CO) family is an important regulator of flowering in photoperiod sensitive plants. But information regarding their role in day neutral plants is limited. We report identification of nine Group I type CONSTANS-like (COL) genes of banana and their characterization for their age dependent, diurnal and tissue-specific expression. Our studies show that the Group I genes are conserved in structure to members in other plants. Expression of these genes shows a distinct circadian regulation with a peak during light period. Developmental stage specific expression reveals high level transcript accumulation of two genes, MaCOL3a and MaCOL3b, well before flowering and until the initiation of flowering. A decrease in their transcript levels after initiation of flowering is followed by an increase in transcription of other members that coincides with the continued development of the inflorescence and fruiting. CO binding cis-elements are observed in at least three FT -like genes in banana suggesting possible CO-FT interactions that might regulate flowering. Distinct tissue specific expression patterns are observed for different family members in mature leaves, apical inflorescence, bracts, fruit skin and fruit pulp suggesting possible roles other than flowering. This is the first exhaustive study of the COL genes belonging to Group I of banana.

  16. Transcripts and MicroRNAs Responding to Salt Stress in Musa acuminata Colla (AAA Group cv. Berangan Roots.

    Directory of Open Access Journals (Sweden)

    Wan Sin Lee

    Full Text Available Physiological responses to stress are controlled by expression of a large number of genes, many of which are regulated by microRNAs. Since most banana cultivars are salt-sensitive, improved understanding of genetic regulation of salt induced stress responses in banana can support future crop management and improvement in the face of increasing soil salinity related to irrigation and climate change. In this study we focused on determining miRNA and their targets that respond to NaCl exposure and used transcriptome sequencing of RNA and small RNA from control and NaCl-treated banana roots to assemble a cultivar-specific reference transcriptome and identify orthologous and Musa-specific miRNA responding to salinity. We observed that, banana roots responded to salinity stress with changes in expression for a large number of genes (9.5% of 31,390 expressed unigenes and reduction in levels of many miRNA, including several novel miRNA and banana-specific miRNA-target pairs. Banana roots expressed a unique set of orthologous and Musa-specific miRNAs of which 59 respond to salt stress in a dose-dependent manner. Gene expression patterns of miRNA compared with those of their predicted mRNA targets indicated that a majority of the differentially expressed miRNAs were down-regulated in response to increased salinity, allowing increased expression of targets involved in diverse biological processes including stress signaling, stress defence, transport, cellular homeostasis, metabolism and other stress-related functions. This study may contribute to the understanding of gene regulation and abiotic stress response of roots and the high-throughput sequencing data sets generated may serve as important resources related to salt tolerance traits for functional genomic studies and genetic improvement in banana.

  17. Training Scientists to be Effective Communicators: AAAS Communicating Science Workshops

    Science.gov (United States)

    Cendes, L.; Lohwater, T.

    2012-12-01

    "Communicating Science: Tools for Scientists and Engineers" is a workshop program developed by AAAS to provide guidance and practice for scientists and engineers in communicating about science with public audiences. The program was launched at the 2008 AAAS Annual Meeting in Boston and has since provided 24 workshops for more than 1,500 scientist and engineer attendees at universities, science society meetings, and government agency labs around the United States. Each interactive workshop targets scientists and engineers specifically and has included content such as message development, defining audience, identifying opportunities for engaging the public, and practice with public presentations and cameras. The workshop format allows for collaborative learning through small-group discussion, resource sharing, and participation in critique of other participants' presentations. Continuous monitoring of the program includes on-site and online surveys and evaluation. On an assessment of workshops from 2008-2010, attendees reported that knowledge gained from the workshop helped in crafting messages about their scientific work for use in communicating with public audiences, and approximately 80 percent of respondents reported participation in communication with a public audience after attending the workshop. Through workshop content and feedback of participating scientists, this presentation will highlight some best practices and resources for scientists who want to take a proactive role in science communication.

  18. Analysis of a Typical Chinese High School Biology Textbook Using the AAAS Textbook Standards

    Science.gov (United States)

    Liang, Ye; Cobern, William W.

    2013-01-01

    The purpose of this study was to evaluate a typical Chinese high school biology textbook using the textbook standards of the American Association for the Advancement of Science (AAAS). The data were composed of three chapters selected from the textbook. Each chapter was analyzed and rated using the AAAS textbook standards. Pearson correlations…

  19. Estimativa das necessidades nutricionais de bananeiras do subgrupo Cavendish cultivadas no Estado de São Paulo Estimate nutrition needs of Cavendish banana trees subgrup grown in the State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Junqueira Teixeira

    2008-06-01

    Full Text Available A acumulação na planta e a exportação de nutrientes pela colheita dos cachos são alguns dos fatores que determinam a necessidade de adubação para a cultura da bananeira. Visando a estimar as quantidades de nutrientes acumulados e exportados por bananeiras do subgrupo Cavendish, nas condições de cultivo do Estado de São Paulo, foram considerados 293 registros de um banco de dados contendo teores de N, P, K, Ca, Mg, S, B, Cu, Fe, Mn e Zn em frutos e em engaços e massa dos cachos das cultivares Grande Naine e Nanicão. Esses registros provieram de experimentos de adubação realizados no Planalto Paulista e no Vale do Ribeira, em áreas irrigadas e de sequeiro, durante sete ciclos de cultivo, variando fontes, doses e formas de aplicação de fertilizantes. Para produzir 40 t ha-1, em média, o nutriente exportado pelos cachos em maior quantidade foi o K (182 kg ha-¹ seguido pelo N (68 kg ha-¹, Mg (10 kg ha-¹, P (8 kg ha-¹, Ca (6 kg ha-¹, S (3 kg ha-¹, Mn (191 g ha-¹, Fe (147 g ha-¹, B (89 g ha-¹, Zn (68 g ha-¹ e Cu (25 g ha-¹. A recomendação de adubação para bananeira para o Estado de São Paulo, aparentemente, subestima a necessidade de K na implantação da cultura e preconiza doses de N muito superiores à exportação de N pelos cachos. Para P, a recomendação está coerente com as necessidades estimadas para a cultura.Fertilizer recommendations for banana take into consideration the accumulation of nutrients in plants and the amount removed in the bunches, among other factors. Nutrient contents accumulated in plants and removed by bunches of Cavendish bananas in the State of São Paulo (Brazil were assessed from a data bank of nutrient concentrations (N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn in bunches (fruits+peduncle and bunch weight with 293 samples. Data bank comprises information from plants of Grand Naine and Giant Cavendish cultivars growing in irrigated and non-irrigated areas, with different sources and

  20. Trapping Effect of Baxi Banana(Musa AAA Cavendish)Pseudostem on Two Banana Weevil Species%巴西蕉假茎对2种香蕉象甲的诱捕效果分析

    Institute of Scientific and Technical Information of China (English)

    李科明; 许桂莺; 彭正强

    2015-01-01

    This study is to determine the effect of Baxi banana pseudostem as attractant to two banana weevil species , and to provide theoretical guidance for control of banana weevil species. Field traps of pseudostem to banana weevils weredeployed for the analysis,meanwhile,indoor selection response of banana weevil to Baxi banana pseudostem was conducted by using double pitfall olfactometer. Significant trapping effects of Baxi banana pseudostem on two ba⁃nana weevils were found by field trapping and number of the trapped banana weevils in five and ten days reaching 8.3~11.3 and 14.7~18.0 individuals per trap,respectively.Indoor selection response results showed that both the two banana weevils showed significant selection effect to the Baxi banana pseudostem when compared with blank control. Baxi banana pseudostem could be used to control the two banana weevil species.%为明确巴西蕉假茎对香蕉假茎象甲和香蕉球茎象甲的诱捕效果,为利用巴西蕉假茎防治香蕉象甲这一农业防治措施提供理论依据,采用假茎田间诱捕试验及室内选择反应试验,研究了巴西蕉假茎对2种香蕉象甲的诱捕效果。田间诱捕试验结果表明,巴西蕉假茎对2种香蕉象甲具有有效的诱捕作用,其5d和10d的诱捕量分别达8.3~11.3和14.7~18.0头/诱捕器;室内选择反应试验结果表明,与空白对照相比,2种香蕉象甲对巴西蕉假茎均表现出显著的选择趋性。因此,巴西蕉假茎可用于蕉园香蕉象甲的诱捕防治。

  1. Aortocaval fistula (ACF) in patients operated for ruptured aortic aneurysm (rAAA)

    DEFF Research Database (Denmark)

    Warning, Karina; Houlind, Kim Christian; Ravn, Hans

    Aortocaval fistula (ACF) in patients operated for Ruptured Acute Aorta Aneurysm (rAAA): A surgical challenge. Introduction Aortocaval fistula is a rare complication of abdominal aortic aneurysm (AAA). It been reported to be present in less than 1% of all AAAs. ACF arise in patients with large...... aneurysms and is typically discovered peroperatively in patients with rAAA. Open surgical treatment is associated with high mortality and morbidity. ACF is a result of spontaneously rupture of large atherosclerotic aneurysms into the inferior vena cava in 80%, 15% arise after trauma and 5% are iatrogenic....... Patients During the last 9 month, we have operated three cases with rAAA and ACF. All patients had an open, acute operation. All ACFs detected during operation. Common for all operations was that the patients had large aneurysms (>10 cm) and extensive bleeding (3-4 l) immediately when the aneurysm...

  2. Instant OpenCV starter

    CERN Document Server

    Dalal, Jayneil

    2013-01-01

    Get to grips with a new technology, understand what it is and what it can do for you, and then get to work with the most important features and tasks.A practical, quick, and hands-on guide for Python developers and hobbyists who want to get started with computer vision with OpenCV.This book is great for developers, hobbyists, and students new to computer vision who are looking to get a good grounding in how to use the OpenCV library. It's assumed that you will have some basic experience in C/C++ programming.

  3. Sonographic Screening for Abdominal Aortic Aneurysm (AAA in Persian Candidates of Coronary Artery Bypass Graft (CABG

    Directory of Open Access Journals (Sweden)

    Sh. Shirani

    2005-08-01

    Full Text Available Introduction & Background: Five percent of the Northern European elder men suffer from AAA. Selective re-pair of unruptured AAA has 5-8% mortality, but mortality rate rises to 50-80% in emergency repair after its rupture. Regarding the fact that coronary artery disease and AAA have similar risk factors such as smoking, atherosclerosis, and hypertension, western references suggest AAA screening in the male candidates of CABG. Other studies have shown that incidence of AAA is lower in Mediterranean ethnic groups than the Northern European origin. The objective of this prospective study is to determine the prevalence of AAA in Persian eth-nic group. Patients & Methods: A total of 717 consecutive candidates of CABG were evaluated by gray scale sonography in Tehran Heart Center from February 2005.untill September 2005. The diameter of aorta was measured in three levels: above superior mesenteric artery, between SMA and renal arteries, and in infrarenal aorta. In each segment the maximum diameter was recorded. Results: Our study population included 192 women (mean age 61.2 years and 525 men (mean age 59.6 years.The prevalence of AAA was 0.52% and 1.9% in women and men respectively. The prevalence of AAA was 5.76% in active or ex-smoker men that is similar to Oxford university study of elderly patients (5%, but lower than Manchester university screening program of CABG male candidates (15%. Conclusion: The incidence of AAA is lower in the Persian ethnic group than the Northern European group. Screening for AAA is not justified in Persian male candidates of CABG except for the smoker subgroup.

  4. Spatial distribution of banana skipper (Erionota thrax L.) (Lepidoptera: Hesperiidae) and its parasitoids in a Cavendish banana plantation, Penang, Malaysia

    Institute of Scientific and Technical Information of China (English)

    JUSTIN N. OKOLLE; MASHHOR MANSOR; ABU HASSAN AHMAD

    2006-01-01

    Spatial distribution of immatures of the banana skipper (Erionota thrax L.) and their parasitisms from three major parasitoids were studied in a Cavendish banana plantation from April 2004 to December 2004. Infestation levels and parasitism ofE. thrax life stages were recorded from bunched plants (BP), flowering plants (FP), preflowered plants (PF),broad leaf followers (BLF) and narrow leaf followers (NLF), as well as on well managed and poorly managed plants. Mean numbers of the immatures and numbers parasitized from the nine blocks in the plantation were fitted to four dispersion indices. Significant numbers of E. thrax immatures and those parasitized by Ooencyrtus erionotae, Cotesia erionotae and Brachymeria albotibialis were recorded from BLF and PF; no eggs were found on BP and FP. Although infestation was higher on well managed plants, only larval parasitism was significandy different. Three of the four indices indicated that eggs and larvae were random while all the indices showed pupae to be clumped. Parasitized eggs and pupae were clumped (4/4 indices) while 3/4 indices revealed a random pattern for parasitized larvae.

  5. Radiation dose assessment of musa acuminata - triploid (AAA)

    International Nuclear Information System (INIS)

    Bananas are radioactive due to the presence of the radioisotope-40K. This imposes a possible health risk to the general public. This study intended to assess the annual equivalent dosages and the annual effective dosage committed by the body. This seeks to benefit the general public, students and researchers, and entrepreneurs. Using atomic absorption spectrophotometry, lakatan banana (Musa acuminata-triploid (AAA), the most purchased variety cultivated in Barangay Adlawon, Cebu City, Philippines, was found to contain 0.53 g of total potassium for every 100 g of its fresh fruit wherein 6.2 x 10-5 g of which is potassium-40. Based on its 40K content banana was calculated to have a radioactivity of 16 Bq/100 g. it was found out that the body is exposed to radiation dosages ranging from 2.8 x 10-3 rem annually by eating 100 g of lakatan bananas everyday. Conversely, it is equivalent to the annual effective dosage of 0.0043 rem; the amount at which the body of an individual is uniformly exposed. However, no or extremely minute health risk was determined by just eating bananas. In fact, to exceed the radiation dose limits set by the International Commission on Radiation Protection, an individual may eat 116 kg of lakatan bananas everyday for a year. Fertilizers may be the major source of the radioisotope - 40K and assimilated by the plants. (author)

  6. An AAA-DDD triply hydrogen-bonded complex easily accessible for supramolecular polymers.

    Science.gov (United States)

    Han, Yi-Fei; Chen, Wen-Qiang; Wang, Hong-Bo; Yuan, Ying-Xue; Wu, Na-Na; Song, Xiang-Zhi; Yang, Lan

    2014-12-15

    For a complementary hydrogen-bonded complex, when every hydrogen-bond acceptor is on one side and every hydrogen-bond donor is on the other, all secondary interactions are attractive and the complex is highly stable. AAA-DDD (A=acceptor, D=donor) is considered to be the most stable among triply hydrogen-bonded sequences. The easily synthesized and further derivatized AAA-DDD system is very desirable for hydrogen-bonded functional materials. In this case, AAA and DDD, starting from 4-methoxybenzaldehyde, were synthesized with the Hantzsch pyridine synthesis and Friedländer annulation reaction. The association constant determined by fluorescence titration in chloroform at room temperature is 2.09×10(7)  M(-1) . The AAA and DDD components are not coplanar, but form a V shape in the solid state. Supramolecular polymers based on AAA-DDD triply hydrogen bonded have also been developed. This work may make AAA-DDD triply hydrogen-bonded sequences easily accessible for stimuli-responsive materials.

  7. Biosynthesis of silver nanoparticles from Cavendish banana peel extract and its antibacterial and free radical scavenging assay: a novel biological approach

    Science.gov (United States)

    Kokila, T.; Ramesh, P. S.; Geetha, D.

    2015-11-01

    Biosynthesis of metallic silver nanoparticles has now become an alternative to physical and chemical approaches. In the present study, silver nanoparticles (AgNPs) were synthesized from Cavendish banana peel extract (CBPE) and characterized by UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Atomic force microscopy (AFM), Field emission scanning electronic microscope (FESEM), Dynamic light scattering (DLS) and zeta potential (ZP). The AgNPs formation was confirmed by UV-visible spectroscopy through color conversion due to surface plasma resonance band at 430 nm. The effect of pH on nanoparticle synthesis was determined by adjusting the various pH of the reaction mixtures. The crystalline nature of nanoparticles was confirmed from the XRD pattern, and the grain size was found to be around 34 nm. To identify the compounds responsible for the bioreduction of Ag+ ion and the stabilization of AgNPs produced, the functional group present in Cavendish banana peel extract was investigated using FTIR. AFM has proved to be very helpful in determining morphological features and the diameter of AgNPs in the range of 23-30 nm was confirmed by FESEM. DLS studies revealed that the average size of AgNPs was found to be around 297 nm. Zeta potential value for AgNPs obtained was -11 mV indicating the moderate stability of synthesized nanoparticles. The antibacterial activity of the nanoparticles was studied against Gram-positive and Gram-negative bacteria. Biosynthesized AgNPs showed a strong DPPH radical and ABTS scavengers compared to the aqueous peel extract of Cavendish banana.

  8. Cavendish, Sueli & Valois, Michelle (Org. Teoria e prática da tradução literária. Recife: Editora UFPE, 2014. 243p.

    Directory of Open Access Journals (Sweden)

    Sonia Ramalho Farias

    2015-12-01

    Full Text Available Critical review of the book Theory and Practice of Literary Translation organized by Sueli Cavendish and Michelle Valois, seeking to learn simultaneously the double objective that sets the collection: the practice of translation and the need to theorize about it through the characteristic disciplinary character of several studies. Some tests were selected from a total of fourteen forming the Anthology. The review aims to detect multiple ways of thinking about translation and the extension of its concept by the various essayists. The text focuses specifically on the interchangeability of translating concepts, interpretation and criticism and dialogue that Translation Studies have with the Literary Theory, Comparative Literature and other disciplines.

  9. C-V uniformity measurements

    International Nuclear Information System (INIS)

    Ion implantation's unique contributions to FET processing are excellent uniformity and repeatability at low dose. These could be verified on product wafers but were difficult to measure on monitor wafers. This paper presents an MOS technique that integrates the C-V curve directly to obtain part of the implanted dose - the partial dose. This technique is fast and insensitive to measurement noise and dot-diameter variation. It uses standard MOS wafers and processing. Neither photo-defined dots nor special substrates are required. Presently the integral CdV technique for partial dose is used in several FET lines to measure both implant repeatability and uniformity. (orig.)

  10. AAA application in diagnosis exams in a large public hospital, RS, Brazil; Aplicacao do AAA na realizacao de exames diagnosticos em um hospital publico de grande porte

    Energy Technology Data Exchange (ETDEWEB)

    Bacelar, A.; Ferret, A.A.; Vanni, S.; Galhardi, M.P.; Lykawka, R., E-mail: abacelar@hepa.ufrgs.br, E-mail: allferret@gmail.com, E-mail: svanni@hepa.ufrgs.br, E-mail: mpgalhardi@gmail.com, E-mail: rlykawka@hepa.ufrgs.br [Hospital de Clinicas de Porto Alegre (HCPA), Porto Alegre, RS (Brazil)

    2013-10-01

    Objective: the initiative AAA - Awareness , Appropriateness and Audit , promotes consciousness ( Awareness) , fitness ( Appropriateness ) and Audit ( Audit) . This paper analyzes the application of the concept in the AAA requests and justifications examinations using ionizing radiation within a large public hospital. Materials and methods: we collected and analyzed data between the years 2011 and 2012, concerning the number of exams performed with the use of radiation and their justifications. After, we sought to raise awareness of the clinical team through training on the risks and benefits of the various modalities of the radiology department and the need to justify the use of ionizing radiation on health. After the data were collected again of test requests for verification of the effectiveness of training. Results: the mean requests that need to be appropriate to the AAA in the last quarter of 2011 was 75 % lower than the average demands of the first quarter, matched against the last two months of 2012 increased by up to four times the number requests that require improvements in relation to the excellent results obtained in July 2012. Conclusion: it is shown in this paper the need of implementing this initiative AAA continuously added to the clinical staff awareness about the risk of the use of ionizing radiation, the appropriateness of the requests of these tests , as well as the control of this process in order to optimize use of ionizing radiation on health.

  11. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    DEFF Research Database (Denmark)

    Ottosson, R O; Hauer, Anna Karlsson; Behrens, C.F.

    2010-01-01

    to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located...... centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6MV. AAA and MC both predict similar perturbations in dose...... distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement...

  12. Cell-Activation by Shear Stresses in Abdominal Aortic Aneurysms (AAA)

    Science.gov (United States)

    Salsac, Anne-Virginie; Sparks, Steven; Chomaz, Jean-Marc; Lasheras, Juan C.

    2003-11-01

    Increasing experimental evidence indicates that low and oscillatory shear stresses promote proliferative, thrombotic, adhesive and inflammatory-mediated degenerative conditions throughout the wall of the aorta. These degenerative conditions have been shown to be involved in the pathogenesis of AAAs, a permanent, localized dilatation of the abdominal aorta. The purpose of this study is to measure both the magnitude and the duration of the shear stresses acting on both the arterial walls and on the blood cells inside AAAs, and to characterize their changes as the AAA enlarges. We conducted a parametric in-vitro study of the pulsatile blood flow in elastic models of AAAs while systematically varying the blood flow parameters, and the geometry of the aneurysm's bulging. The instantaneous flow characteristic inside the AAA was measured using DPIV at a sampling rate of 15 Hertz. A "cell-activation parameter" defined as the integral of the product of the magnitude of the shear stress and the time during which the stress acts was computed along each of the blood cell pathlines. The Lagrangian tracking of the blood cells shows that a large majority of them are subjected first to very high level of shear-induced "cell-activation" while later on they are entrained in regions of stasis where their residence time can increase up to several cardiac cycles. This cell-activation followed by the entrainment in low shear regions creates the optimal cell-adhesive and inflammatory-mediated degenerative conditions that are postulated to play an important role in the etiology and progressive enlargement of AAAs.

  13. Norms for multivariate diagnosis of nutrient imbalance in the East African highland bananas (musa spp.aaa)

    NARCIS (Netherlands)

    Wairegi, L.; Asten, van P.

    2011-01-01

    Despite low yields and soil fertility problems, fertilizer use in the East African Highland banana (AAA-EA) production is absent. High fertilizer costs increase the need for site-specific fertilizer recommendations that address deficiencies. This study aimed to derive and compare norms for AAA-EA ba

  14. The two faces of hydrogen-bond strength on triple AAA-DDD arrays.

    Science.gov (United States)

    Lopez, Alfredo Henrique Duarte; Caramori, Giovanni Finoto; Coimbra, Daniel Fernando; Parreira, Renato Luis Tame; da Silva, Éder Henrique

    2013-12-01

    Systems that are connected through multiple hydrogen bonds are the cornerstone of molecular recognition processes in biology, and they are increasingly being employed in supramolecular chemistry, specifically in molecular self-assembly processes. For this reason, the effects of different substituents (NO2, CN, F, Cl, Br, OCH3 and NH2) on the electronic structure, and consequently on the magnitude of hydrogen bonds in triple AAA-DDD arrays (A=acceptor, D=donor) were evaluated in the light of topological [electron localization function (ELF) and quantum theory of atoms in molecules (QTAIM)], energetic [Su-Li energy-decomposition analysis (EDA) and natural bond orbital analysis (NBO)], and geometrical analysis. The results based on local H-bond descriptors (geometries, QTAIM, ELF, and NBO) indicate that substitutions with electron-withdrawing groups on the AAA module tend to strengthen, whereas electron-donating substituents tend to weaken the covalent character of the AAA-DDD intermolecular H-bonds, and also indicate that the magnitude of the effect is dependent on the position of substitution. In contrast, Su-Li EDA results show an opposite behavior when compared to local H-bond descriptors, indicating that electron-donating substituents tend to increase the magnitude of H-bonds in AAA-DDD arrays, and thus suggesting that the use of local H-bond descriptors describes the nature of H bonds only partially, not providing enough insight about the strength of such H bonds.

  15. AAA application in diagnosis exams in a large public hospital, RS, Brazil

    International Nuclear Information System (INIS)

    Objective: the initiative AAA - Awareness , Appropriateness and Audit , promotes consciousness ( Awareness) , fitness ( Appropriateness ) and Audit ( Audit) . This paper analyzes the application of the concept in the AAA requests and justifications examinations using ionizing radiation within a large public hospital. Materials and methods: we collected and analyzed data between the years 2011 and 2012, concerning the number of exams performed with the use of radiation and their justifications. After, we sought to raise awareness of the clinical team through training on the risks and benefits of the various modalities of the radiology department and the need to justify the use of ionizing radiation on health. After the data were collected again of test requests for verification of the effectiveness of training. Results: the mean requests that need to be appropriate to the AAA in the last quarter of 2011 was 75 % lower than the average demands of the first quarter, matched against the last two months of 2012 increased by up to four times the number requests that require improvements in relation to the excellent results obtained in July 2012. Conclusion: it is shown in this paper the need of implementing this initiative AAA continuously added to the clinical staff awareness about the risk of the use of ionizing radiation, the appropriateness of the requests of these tests , as well as the control of this process in order to optimize use of ionizing radiation on health

  16. Fluid Characteristics in Abdominal Aortic Aneurysms (AAAs) and Its Correlation to Thrombus Formation

    Science.gov (United States)

    Tang, Rubing; Bar-Yoseph, Pinhas Z.; Lasheras, Juan

    2008-11-01

    It has been observed that most large Abdominal Aortic Aneurysms (AAAs) develop an intraluminal thrombus as they progressively enlarge. Previous studies have suggested that the build up of the thrombus may be associated with the altered hemodynamic patterns that arise inside the AAA. We have performed a parametrical computational study of the flow patterns inside enlarging AAA to investigate the possible mechanism controlling the thrombus formation. Pulsatile blood flows were simulated in idealized models of fusiform aneurysms with different dilatation ratios and the effects of shear-activated platelet accumulation and platelet/wall interaction were evaluated based on the calculated flow fields. The platelet activation level (PAL) was determined by computing the integral over time of flow shear stresses exerted over the platelets as they are transported throughout the aneurysm. Our results have shown that the values of PAL in AAAs are in fact smaller than the maximum value obtained in a healthy abdominal aorta. However, we show that the transportation of blood cells towards the wall and the formation of stagnation points on the aneurysm's wall play more significant roles in thrombus formation than PAL.

  17. Effects of doping and bias voltage on the screening in AAA-stacked trilayer graphene

    Science.gov (United States)

    Mohammadi, Yawar; Moradian, Rostam; Shirzadi Tabar, Farzad

    2014-09-01

    We calculate the static polarization of AAA-stacked trilayer graphene (TLG) and study its screening properties within the random phase approximation (RPA) in all undoped, doped and biased regimes. We find that the static polarization of undoped AAA-stacked TLG is a combination of the doped and undoped single-layer graphene static polarization. This leads to an enhancement of the dielectric background constant along a Thomas-Fermi screening with the Thomas-Fermi wave vector which is independent of carrier concentrations and a 1/r3 power law decay for the long-distance behavior of the screened Coulomb potential. We show that effects of a bias voltage can be taken into account by a renormalization of the interlayer hopping energy to a new bias-voltage-dependent value, indicating screening properties of AAA-stacked TLG can be tuned electrically. We also find that screening properties of doped AAA-stacked TLG, when μ exceeds √{2}γ, are similar to that of doped SLG only depending on doping. While for μ<√{2}γ, its screening properties are combination of SLG and AA-stacked bilayer graphene screening properties and they are determined by doping and the interlayer hopping energy.

  18. Sexual Dysfunction After Conventional and Endovascular AAA Repair: Results of the DREAM Trial.

    NARCIS (Netherlands)

    Prinssen, M.; Buskens, E.; Nolthenius, R.P.T.; Sterkenburg, S. van; Teijink, J.A.; Blankensteijn, J.D.

    2004-01-01

    Purpose: To assess sexual function in the first postoperative year after elective endovascular aneurysm repair (EVAR) and open repair (OR) of abdominal aortic aneurysm (AAA).Methods: In the Dutch Randomized Endovascular Aneurysm Management (DREAM) trial, 153 patients (141 men; mean age 71 years, ran

  19. The mouse fidgetin gene defines a new role for AAA family proteins in mammalian development.

    Science.gov (United States)

    Cox, G A; Mahaffey, C L; Nystuen, A; Letts, V A; Frankel, W N

    2000-10-01

    The mouse mutation fidget arose spontaneously in a heterogeneous albino stock. This mutant mouse is characterized by a side-to-side head-shaking and circling behaviour, due to reduced or absent semicircular canals. Fidget mice also have small eyes, associated with cell-cycle delay and insufficient growth of the retinal neural epithelium, and lower penetrance skeletal abnormalities, including pelvic girdle dysgenesis, skull bone fusions and polydactyly. By positional cloning, we found the gene mutated in fidget mice, fidgetin (Fign), which encodes a new member of the 'meiotic' or subfamily-7 (SF7; ref. 7) group of ATPases associated with diverse cellular activities (AAA proteins). We also discovered two closely related mammalian genes. AAA proteins are molecular chaperones that facilitate a variety of functions, including membrane fusion, proteolysis, peroxisome biogenesis, endosome sorting and meiotic spindle formation, but functions for the SF7 AAA proteins are largely unknown. Fidgetin is the first mutant AAA protein found in a mammalian developmental mutant, thus defining a new role for these proteins in embryonic development.

  20. Dilatation of the proximal neck of infrarenal aortic aneurysms after endovascular AAA repair

    NARCIS (Netherlands)

    Wever, JJ; de Nie, AJ; Blankensteijn, JD; Broeders, IAMJ; Mail, WPTM; Eikelboom, BC

    2000-01-01

    Objectives: to assess size changes of the proximal aortic neck after endograft placement. Methods: since 1994, 54 consecutive patients have undergone abdominal aortic aneurysm (AAA) repair with the Endovascular Technologies (EVT) endograft. The study group comprised the 33 patients who had completed

  1. Asymmetric processing of a substrate protein in sequential allosteric cycles of AAA+ nanomachines

    Science.gov (United States)

    Kravats, Andrea N.; Tonddast-Navaei, Sam; Bucher, Ryan J.; Stan, George

    2013-09-01

    Essential protein quality control includes mechanisms of substrate protein (SP) unfolding and translocation performed by powerful ring-shaped AAA+ (ATPases associated with various cellular activities) nanomachines. These SP remodeling actions are effected by mechanical forces imparted by AAA+ loops that protrude into the central channel. Sequential intra-ring allosteric motions, which underlie repetitive SP-loop interactions, have been proposed to comprise clockwise (CW), counterclockwise (CCW), or random (R) conformational transitions of individual AAA+ subunits. To probe the effect of these allosteric mechanisms on unfoldase and translocase functions, we perform Langevin dynamics simulations of a coarse-grained model of an all-alpha SP processed by the single-ring ClpY ATPase or by the double-ring p97 ATPase. We find that, in all three allosteric mechanisms, the SP undergoes conformational transitions along a common set of pathways, which reveals that the active work provided by the ClpY machine involves single loop-SP interactions. Nevertheless, the rates and yields of SP unfolding and translocation are controlled by mechanism-dependent loop-SP binding events, as illustrated by faster timescales of SP processing in CW allostery compared with CCW and R allostery. The distinct efficacy of allosteric mechanisms is due to the asymmetric collaboration of adjacent subunits, which involves CW-biased structural motions of AAA+ loops and results in CW-compatible torque applied onto the SP. Additional simulations of mutant ClpY rings, which render a subset of subunits catalytically-defective or reduce their SP binding affinity, reveal that subunit-based conformational transitions play the major role in SP remodeling. Based on these results we predict that the minimally functional AAA+ ring includes three active subunits, only two of which are adjacent.

  2. CV and CM chondrite impact melts

    Science.gov (United States)

    Lunning, Nicole G.; Corrigan, Catherine M.; McSween, Harry Y.; Tenner, Travis J.; Kita, Noriko T.; Bodnar, Robert J.

    2016-09-01

    Volatile-rich and typically oxidized carbonaceous chondrites, such as CV and CM chondrites, potentially respond to impacts differently than do other chondritic materials. Understanding impact melting of carbonaceous chondrites has been hampered by the dearth of recognized impact melt samples. In this study we identify five carbonaceous chondrite impact melt clasts in three host meteorites: a CV3red chondrite, a CV3oxA chondrite, and a regolithic howardite. The impact melt clasts in these meteorites respectively formed from CV3red chondrite, CV3oxA chondrite, and CM chondrite protoliths. We identified these impact melt clasts and interpreted their precursors based on their texture, mineral chemistry, silicate bulk elemental composition, and in the case of the CM chondrite impact melt clast, in situ measurement of oxygen three-isotope signatures in olivine. These impact melts typically contain euhedral-subhedral olivine microphenocrysts, sometimes with relict cores, in glassy groundmasses. Based on petrography and Raman spectroscopy, four of the impact melt clasts exhibit evidence for volatile loss: these melt clasts either contain vesicles or are depleted in H2O relative to their precursors. Volatile loss (i.e., H2O) may have reduced the redox state of the CM chondrite impact melt clast. The clasts that formed from the more oxidized precursors (CV3oxA and CM chondrites) exhibit phase and bulk silicate elemental compositions consistent with higher intrinsic oxygen fugacities relative to the clast that formed from a more reduced precursor (CV3red chondrite). The mineral chemistries and assemblages of the CV and CM chondrite impact melt clasts identified here provide a template for recognizing carbonaceous chondrite impact melts on the surfaces of asteroids.

  3. Substituent effects in double-helical hydrogen-bonded AAA-DDD complexes.

    Science.gov (United States)

    Wang, Hong-Bo; Mudraboyina, Bhanu P; Wisner, James A

    2012-01-27

    Two series of DDD and AAA hydrogen-bond arrays were synthesized that form triply-hydrogen-bonded double-helical complexes when combined in CDCl(3) solution. Derivatization of the DDD arrays with electron-withdrawing groups increases the complex association constants by up to a factor of 30 in those arrays examined. Derivatization of the AAA arrays with electron donating substituents reveals a similar magnitude effect on the complex stabilities. The effect of substitution on both types of arrays are modeled quite satisfactorily (R(2) > 0.96 in all cases) as free energy relationships with respect to the sums of their Hammett substituent constants. In all, the complex stabilities can be manipulated over more than three orders of magnitude (>20 kJ mol(-1)) using this type of modification.

  4. Mutations of the AAAS gene in an Indian family with Allgrove's syndrome

    Institute of Scientific and Technical Information of China (English)

    Ashis Mukhopadhya; Sumita Danda; Angela Huebner; Ashok Chacko

    2006-01-01

    The triple A or Allgrove's syndrome is an autosomal recessive disorder characterized by the triad of achalasia cardia, alacrima and ACTH resistant adrenocortical insufficiency. Mutations of the Achalasia-Addisonianism-Alacrima-Syndrome (AAAS) gene on chromosome 12q13 are associated with this syndrome. We report an Indian family where two siblings were homozygous for a known mutation of the AAAS gene and presented with the classical triad of symptoms. The mother and the brother were heterozygous and asymptomatic. The affected siblings had iron deficiency anemia and the younger sister had pes cavus and palmoplantar keratosis.Neurological symptoms were absent in both affected children. Recognition of this syndrome can lead to early treatment of adrenal insufficency and genetic counselling.

  5. Geometrical factors influencing the hemodynamic behavior of the AAA stent grafts: essentials for the clinician.

    Science.gov (United States)

    Georgakarakos, Efstratios; Argyriou, Christos; Schoretsanitis, Nikolaos; Ioannou, Chris V; Kontopodis, Nikolaos; Morgan, Robert; Tsetis, Dimitrios

    2014-12-01

    Endovascular aneurysm repair (EVAR) is considered to be the treatment of choice for abdominal aortic aneurysms (AAA). Despite the initial technical success, EVAR is amenable to early and late complications, among which the migration of the endograft (EG) with subsequent proximal endoleak (Type Ia) leads to repressurization of the AAA sac, exposure to excessive wall stress, and, hence, to potential rupture. This article discusses the influence that certain geometrical factors, such as neck angulation, iliac bifurcation, EG curvature, neck-to-iliac diameter, and length ratios, as well as iliac limbs configuration can exert on the hemodynamic behavior of the EGs. The information provided could help both clinicians and EG manufacturers towards further development and improvement of EG designs and better operational planning. PMID:24938906

  6. SFM-FDTD analysis of triangular-lattice AAA structure: Parametric study of the TEM mode

    Science.gov (United States)

    Hamidi, M.; Chemrouk, C.; Belkhir, A.; Kebci, Z.; Ndao, A.; Lamrous, O.; Baida, F. I.

    2014-05-01

    This theoretical work reports a parametric study of enhanced transmission through annular aperture array (AAA) structure arranged in a triangular lattice. The effect of the incidence angle in addition to the inner and outer radii values on the evolution of the transmission spectra is carried out. To this end, a 3D Finite-Difference Time-Domain code based on the Split Field Method (SFM) is used to calculate the spectral response of the structure for any angle of incidence. In order to work through an orthogonal unit cell which presents the advantage to reduce time and space of computation, special periodic boundary conditions are implemented. This study provides a new modeling of AAA structures useful for producing tunable ultra-compact devices.

  7. Indications for and outcome of open AAA repair in the endovascular era.

    Science.gov (United States)

    Wieker, Carola M; Spazier, Max; Böckler, Dittmar

    2016-04-01

    The benefits, safety and efficacy of endovascular aortic aneurysm repair (EVAR) is well documented and intensively reported in multiple randomized trials and meta-analysis. Therefore, EVAR became the first choice of abdominal aortic aneurysms (AAA) treatment in almost 70-100% of patients. Consecutively, open repair (OR) is performed less frequently in morphologically preselected patients. Anatomical condition remains the most important factor for indication for OR. Especially unfavorable intrarenal landing zone based on difficult neck anatomy like very short neck or excessive neck angulation is still the most predictive factor. Furthermore, patients presenting additional iliac aneurysms, aortoiliac occlusive disease or variations of renal arteries are recommended for OR. Randomized trials like EVAR 1, DREAM and OVER from the year 2004/2005 and 2009 showed lower 30-day mortality rates in EVAR compared to OR. However, the late mortality rates after two years became equal in both treatment options. Furthermore, reinterventions after EVAR occur more frequently than after OR. Analysis from our own data showed a higher 30-day mortality in the patients who underwent OR in the endovascular era (15% vs. 2.5%), however the number of emergency open AAA repair because of ruptured aneurysms was much higher in the endovascular era (32.5% vs. 5%). In conclusion, treatment of AAA has changed in the past decade. Nevertheless OR of AAA still remains as a safe and durable method in experienced surgeons, even in the endovascular era. High volume centres are needed to offer the best patients' treatment providing the best postoperative outcome. Therefore OR must remain a part of fellowship training in the future. To decide the best treatment option many facts like patients' fitness and preference or finally the anatomic suitability for endovascular repair have to be considered. PMID:26822580

  8. Functional characterization of fidgetin, an AAA-family protein mutated in fidget mice.

    Science.gov (United States)

    Yang, Yan; Mahaffey, Connie L; Bérubé, Nathalie; Nystuen, Arne; Frankel, Wayne N

    2005-03-10

    The mouse fidget mutation is an autosomal recessive mutation that renders reduced or absent semicircular canals, microphthalmia, and various skeletal abnormalities to affected mice. We previously identified the defective gene which encodes fidgetin, a new member of the ATPases associated with diverse cellular activities (AAA proteins). Here, we report on the subcellular localization of fidgetin as well as that of two closely related proteins, fidgetin-like 1 and fidgetin-like 2. Epitope-tagging and immunostaining revealed that both fidgetin and fidgetin-like 2 were predominantly localized to the nucleus, whereas fidgetin-like 1 was both nuclear and cytoplasmic. Furthermore, deletion studies identified a putative bipartite nuclear localization signal in the middle portion of the fidgetin protein. Since AAA proteins are known to form functional hetero- or homo-hexamers, we used reciprocal immunoprecipitation to examine the potential interaction among these proteins. We found that fidgetin interacted with itself and this specific interaction was abolished when either the N- or C-terminus of the protein was truncated. Taken together, our results suggest that fidgetin is a nuclear AAA-family protein with the potential to form homo-oligomers, thus representing the first step towards the elucidation of fidgetin's cellular function and the disease mechanism in fidget mutant mice.

  9. CV/TCR Control System Perspectives

    CERN Document Server

    Morodo-Testa, M C

    2000-01-01

    At present, 35% of the CERN technical infrastructure points being monitored by the TCR are related to CV facilities. The adaptation of the CV systems to the LHC and the new experimental areas is leading to a re-engineering of all their control and monitoring systems in order to reach the more demanding requirements of the LHC era in terms of reliability and efficiency. In parallel, the TCR is redefining its role and establishing the means to continue providing a high-quality monitoring service to the community of CERN. The common aim is to achieve a smooth evolution from the existing systems to the new ones during the whole upgrading process. The integration of the CV control systems into a coherent TCR control and monitoring system requires the development and implementation of the appropriate technical solutions by a joint team in charge of co-ordinating the individual efforts and resources.

  10. Experimental and computational studies on the flow fields in aortic aneurysms associated with deployment of AAA stent-grafts

    Institute of Scientific and Technical Information of China (English)

    Xiwen Zhang; Zhaohui Yao; Yan Zhang; Shangdong Xu

    2007-01-01

    Pulsatile flow fields in rigid abdominal aortic aneurysm (AAA) models were investigated numerically, and the simulation results are found in good agreement with particle image velocimetry (PIV) measurements. There are one or more vortexes in the AAA bulge, and a fairly high wall shear stress exists at the distal end, and thus the AAA is in danger of rupture. Medical treatment consists of inserting a vascular stent-graft in the AAA, which would decrease the blood impact to the inner walls and reduce wall shear stress so that the rupture could be prevented. A new computational model, based on porous medium model, was developed and results are documented. The rapeutic effect of the stent-graft was verified numerically with the new model.

  11. Intraoperative platelet and plasma improves survival in patients operated for a rAAA: a follow-up evaluation

    DEFF Research Database (Denmark)

    Johansson, Per Ingemar; Swiatek, F.; Jorgensen, L.;

    2008-01-01

    OBJECTIVES: Continued haemorrhage remains a significant contributor to mortality in massively transfused patients. We found that early administration of platelets and plasma reduced mortality from 54% to 36% in rAAA patients. The aim of the present evaluation was to evaluate whether reduced...... mortality in rAAA patients related to a pro-active transfusion therapy is maintained. DESIGN: Single-centre observational study. METHODS: Mortality of patients operated for rAAA 2006-07 was compared to that of patients operated 2004-05 (intervention group; n=50) and 2002-04 (control group, n=82). RESULTS......: 64 consecutive patients with rAAA received, similar to the intervention group, more platelets (5 and 4 vs. 0 units, P

  12. OpenCV for secret agents

    CERN Document Server

    Howse, Joseph

    2015-01-01

    This book is for programmers who want to expand their skills by building fun, smart, and useful systems with OpenCV. The projects are ideal in helping you to think creatively about the uses of computer vision, natural user interfaces, and ubiquitous computers (in your home, car, and hand).

  13. NASA Astrophysics E/PO Impact: NASA SOFIA AAA Program Evaluation Results

    Science.gov (United States)

    Harman, Pamela; Backman, Dana E.; Clark, Coral; Inverness Research Sofia Aaa Evaluation Team, Wested Sofia Aaa Evaluation Team

    2015-01-01

    SOFIA is an airborne observatory, studying the universe at infrared wavelengths, capable of making observations that are impossible for even the largest and highest ground-based telescopes. SOFIA also inspires the development of new scientific instrumentation and fosters the education of young scientists and engineers.SOFIA is an 80% - 20% partnership of NASA and the German Aerospace Center (DLR), consisting of an extensively modified Boeing 747SP aircraft carrying a reflecting telescope with an effective diameter of 2.5 meters (100 inches). The SOFIA aircraft is based at NASA Armstrong Flight Research Center, Building 703, in Palmdale, California. The Science Program and Outreach Offices are located at NASA Ames Research center. SOFIA is a program in NASA's Science Mission Directorate, Astrophysics Division.Data will be collected to study many different kinds of astronomical objects and phenomena, including star cycles, solar system formation, identification of complex molecules in space, our solar system, galactic dust, nebulae and ecosystems.Airborne Astronomy Ambassador (AAA) Program:The SOFIA Education and Communications program exploits the unique attributes of airborne astronomy to contribute to national goals for the reform of science, technology, engineering, and math (STEM) education, and to elevate public scientific and technical literacy.The AAA effort is a professional development program aspiring to improve teaching, inspire students, and inform the community. To date, 55 educators from 21 states; Cycles 0, 1 and 2; have completed their astronomy professional development and their SOFIA science flight experience. Evaluation has confirmed the program's positive impact on the teacher participants, on their students, and in their communities. The inspirational experience has positively impacted their practice and career trajectory. AAAs have incorporated content knowledge and specific components of their experience into their curricula, and have given

  14. Morphological State as a Predictor for Reintervention and Mortality After EVAR for AAA

    Energy Technology Data Exchange (ETDEWEB)

    Ohrlander, Tomas [Eksjoe County Hospital (Sweden); Dencker, Magnus [Malmoe University Hospital, Department of Clinical Physiology and Nuclear Medicine (Sweden); Acosta, Stefan, E-mail: stefan.acosta@telia.com [Malmoe University Hospital, Vascular Center Malmoe-Lund (Sweden)

    2012-10-15

    Purpose: This study was designed to assess aorto-iliac morphological characteristics in relation to reintervention and all-cause long-term mortality in patients undergoing standard EVAR for infrarenal AAA. Methods: Patients treated with EVAR (Zenith{sup Registered-Sign} Stentgrafts, Cook) between May 1998 and February 2006 were prospectively enrolled in a computerized database where comorbidities and preoperative aneurysm morphology were entered. Reinterventions and mortality were checked until December 1, 2010. Median follow-up time was 68 months. Results: A total of 304 patients were included, of which 86% were men. Median age was 74 years. The reintervention rate was 23.4% (71/304). A greater diameter of the common iliac artery (p = 0.037; hazard ratio (HR) 1.037 [1.002-1.073]) was an independent factor for an increased number of reinterventions. The 30-day mortality rate was 3.0% (9/304). Aneurysm-related deaths due to AAA occurred in 4.9% (15/304). Five patients died due to a concomitant ruptured thoracic aortic aneurysm. The mortality until end of follow-up was 54.3% (165/304). The proportion of deaths caused by vascular diseases was 61.6%. The severity of angulation of the iliac arteries (p = 0.014; HR 1.018 [95% confidence interval (CI) 1.004-1.033]) and anemia (p = 0.044; HR 2.79 [95% CI 1.029-7.556]) remained as independent factors associated with all-cause long-term mortality. The crude reintervention-free survival rate at 1, 3, and 5 years was 84.5%, 64.8%, and 51.6%, respectively. Conclusions: The initial aorto-iliac morphological state in patients scheduled for standard EVAR for AAA seems to be strongly related to the need for reinterventions and long-term mortality.

  15. SU-E-P-16: A Feasibility Study of Using Eclipse AAA for SRS Treatement

    Energy Technology Data Exchange (ETDEWEB)

    Lim, S; LoSasso, T [Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    2015-06-15

    Purpose: To commission Varian Eclipse AAA for SRS treatment and compare the accuracy with Brainlab iPlan system for clinical cases measured with radiochromic film. Methods: A 6MV AAA clinical model for a Varian TrueBeam STx is used as baseline. The focal spot and field size of the baseline model(BASE) are (1.75,0.75) and 40×40cm{sup 2} respectively. Maximum field sizes, output factors(S{sub t}), FWHM focal spot and secondary source sizes are systematically adjusted to obtain an optimized model(OPT) by comparing the calculated PDD’s, profiles, and output factors with measurements taken with a stereotactic diode(SD) and, cc01 and cc04 ion chambers in Blue Phantom. In-phantom dose distributions of clinical SRS fields are calculated using the OPT and the clinical Brainlab iPlan pencil-beam. Within the 90% isodose-line(ROI), the average dose difference between the calculations and radiochromic film measurements are assessed. Results: The maximum field, focal spot and secondary source sizes for the OPT are 15×15cm{sup 2}, (0,0), and 32.3mm respectively. The OPT St input at 1.0 and 2.0cm fields are increased by 4.5% and 1.5% from BASE. The calculated output of the BASE and OPT underestimate by 16.1%–3.2% respectively at 0.5×0.5cm{sup 2} field and 3.1%−0.02% respectively at 1.0×1.0cm{sup 2} field. The depth doses at 10cm are within 3.5% and 0.4% of measurements for 0.5×0.5 and 1.0×1.0cm{sup 2}. The ROI dose of OPT and iPlan are within 1.6% and 0.6% of film measurements for 3.0cm clinical fields. For 1.0cm fields, the ROI dose of OPT underestimate 0.0–2.0% and iPlan overestimates 1.7–2.9% relative to measurements. Conclusion: The small field dose calculation of Eclipse AAA algorithm can be significantly improved by carefully adjusting the input parameters. The larger deviation of the OPT for 0.5×0.5cm{sup 2} field from measurements can be attributed to the lowest 1.0cm field size input limit of AAA. The OPT compares reasonably well with the iPlan pencil

  16. SU-E-P-16: A Feasibility Study of Using Eclipse AAA for SRS Treatement

    International Nuclear Information System (INIS)

    Purpose: To commission Varian Eclipse AAA for SRS treatment and compare the accuracy with Brainlab iPlan system for clinical cases measured with radiochromic film. Methods: A 6MV AAA clinical model for a Varian TrueBeam STx is used as baseline. The focal spot and field size of the baseline model(BASE) are (1.75,0.75) and 40×40cm2 respectively. Maximum field sizes, output factors(St), FWHM focal spot and secondary source sizes are systematically adjusted to obtain an optimized model(OPT) by comparing the calculated PDD’s, profiles, and output factors with measurements taken with a stereotactic diode(SD) and, cc01 and cc04 ion chambers in Blue Phantom. In-phantom dose distributions of clinical SRS fields are calculated using the OPT and the clinical Brainlab iPlan pencil-beam. Within the 90% isodose-line(ROI), the average dose difference between the calculations and radiochromic film measurements are assessed. Results: The maximum field, focal spot and secondary source sizes for the OPT are 15×15cm2, (0,0), and 32.3mm respectively. The OPT St input at 1.0 and 2.0cm fields are increased by 4.5% and 1.5% from BASE. The calculated output of the BASE and OPT underestimate by 16.1%–3.2% respectively at 0.5×0.5cm2 field and 3.1%−0.02% respectively at 1.0×1.0cm2 field. The depth doses at 10cm are within 3.5% and 0.4% of measurements for 0.5×0.5 and 1.0×1.0cm2. The ROI dose of OPT and iPlan are within 1.6% and 0.6% of film measurements for 3.0cm clinical fields. For 1.0cm fields, the ROI dose of OPT underestimate 0.0–2.0% and iPlan overestimates 1.7–2.9% relative to measurements. Conclusion: The small field dose calculation of Eclipse AAA algorithm can be significantly improved by carefully adjusting the input parameters. The larger deviation of the OPT for 0.5×0.5cm2 field from measurements can be attributed to the lowest 1.0cm field size input limit of AAA. The OPT compares reasonably well with the iPlan pencil-beam and measurements

  17. Earth, Moon, Sun, and CV Accretion Disks

    CERN Document Server

    Montgomery, M M

    2009-01-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting Cataclysmic Variable (CV) Dwarf Novae systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar and black hole systems. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our a...

  18. Dissection of Axial-Pore Loop Function during Unfolding and Translocation by a AAA+ Proteolytic Machine

    Directory of Open Access Journals (Sweden)

    Ohad Iosefson

    2015-08-01

    Full Text Available In the axial channels of ClpX and related hexameric AAA+ protein-remodeling rings, the pore-1 loops are thought to play important roles in engaging, mechanically unfolding, and translocating protein substrates. How these loops perform these functions and whether they also prevent substrate dissociation to ensure processive degradation by AAA+ proteases are open questions. Using ClpX pore-1-loop variants, single-molecule force spectroscopy, and ensemble assays, we find that the six pore-1 loops function synchronously to grip and unfold protein substrates during a power stroke but are not important in preventing substrate slipping between power strokes. The importance of grip strength is task dependent. ClpX variants with multiple mutant pore-1 loops translocate substrates as well as the wild-type enzyme against a resisting force but show unfolding defects and a higher frequency of substrate release. These problems are magnified for more mechanically stable target proteins, supporting a threshold model of substrate gripping.

  19. Going the distance: validation of Acuros and AAA at an extended SSD of 400 cm.

    Science.gov (United States)

    Lamichhane, Narottam; Patel, Vivek N; Studenski, Matthew T

    2016-01-01

    Accurate dose calculation and treatment delivery is essential for total body irradia-tion (TBI). In an effort to verify the accuracy of TBI dose calculation at our institu-tion, we evaluated both the Varian Eclipse AAA and Acuros algorithms to predict dose distributions at an extended source-to-surface distance (SSD) of 400 cm. Measurements were compared to calculated values for a 6 MV beam in physical and virtual phantoms at 400 cm SSD using open beams for both 5 × 5 and 40 × 40cm2 field sizes. Inline and crossline profiles were acquired at equivalent depths of 5 cm, 10 cm, and 20 cm. Depth-dose curves were acquired using EBT2 film and an ion chamber for both field sizes. Finally, a RANDO phantom was used to simulate an actual TBI treatment. At this extended SSD, care must be taken using the planning system as there is good relative agreement between measured and calculated profiles for both algorithms, but there are deviations in terms of the absolute dose. Acuros has better agreement than AAA in the penumbra region. PMID:27074473

  20. OGLE16aaa - a Signature of a Hungry Super Massive Black Hole

    CERN Document Server

    Wyrzykowski, Łukasz; Kostrzewa-Rutkowska, Z; Hamanowicz, A; Jonker, P G; Arcavi, I; Guillochon, J; Brown, P J; Kozłowski, S; Udalski, A; Szymański, M K; Soszyński, I; Poleski, R; Pietrukowicz, P; Skowron, J; Mróz, P; Ulaczyk, K; Pawlak, M; Rybicki, K A; Greiner, J; Krühler, T; Bolmer, J

    2016-01-01

    We present the discovery and first three months of follow-up observations of a currently on-going unusual transient detected by the OGLE-IV survey, located in the centre of a galaxy at redshift z=0.1655. The long rise to absolute magnitude of -20.5 mag, slow decline, very broad He and H spectral features make OGLE16aaa similar to other optical/UV Tidal Disruption Events (TDEs). Weak narrow emission lines in the spectrum and archival photometric observations suggest the host galaxy is a weak-line Active Galactic Nucleus (AGN), which has been accreting at higher rate in the past. OGLE16aaa, along with SDSS J0748, seems to form a sub-class of TDEs by weakly or recently active supermassive black holes (SMBHs). This class might bridge the TDEs by quiescent SMBHs and flares observed as "changing-look QSOs", if we interpret the latter as TDEs. If this picture is true, the previously applied requirement for identifying a flare as a TDE that it had to come from an inactive nucleus, could be leading to observational bi...

  1. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome.

    Science.gov (United States)

    Förster, Friedrich; Lasker, Keren; Beck, Florian; Nickell, Stephan; Sali, Andrej; Baumeister, Wolfgang

    2009-10-16

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) is in excellent agreement with the recently identified base-precursor complexes formed during the assembly of the RP. Furthermore, the atomic CP-AAA-ATPase model suggests that the assembly chaperone Nas6 facilitates CP-RP association by enhancing the shape complementarity between Rpt3 and its binding CP alpha subunits partners.

  2. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome

    Energy Technology Data Exchange (ETDEWEB)

    Foerster, Friedrich [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany); Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Lasker, Keren [Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Blavatnik School of Computer Science, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv (Israel); Beck, Florian; Nickell, Stephan [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany); Sali, Andrej [Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Baumeister, Wolfgang, E-mail: baumeist@biochem.mpg.de [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany)

    2009-10-16

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) is in excellent agreement with the recently identified base-precursor complexes formed during the assembly of the RP. Furthermore, the atomic CP-AAA-ATPase model suggests that the assembly chaperone Nas6 facilitates CP-RP association by enhancing the shape complementarity between Rpt3 and its binding CP alpha subunits partners.

  3. Effects of the Transient Blood Flow-Wall Interaction on the Wall Stress Distribution in Abdominal Aortic Aneurysm (AAA)

    Science.gov (United States)

    Tang, Rubing; Geindreau, Christian; Lasheras, Juan

    2006-11-01

    Our static finite element analysis (FEA) of both idealized and real clinical models has shown that the maximum diameter and asymmetry have substantial influence on the AAA wall stress distribution. The thrombus inside the AAA was also found to reduce the magnitude of the wall stresses. To achieve a better understanding of the wall stress distribution in real AAAs, a dynamic FEA was also performed. We considered models, both symmetric and non-symmetric, in which the aorta is assumed isotropic with nonlinear material properties. For the limiting case of rigid walls, the evolution of the flow pattern and the wall shear stresses due to fluid flow at different stages of cardiac cycle predicted by our simulations are compared with experimental results obtained in in-vitro models. A good agreement is found between both results. Finally, we have extended the analysis to the physiologically correct case of deformable walls and characterized the transient effects on the wall stresses.

  4. Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state

    Science.gov (United States)

    Morito, Daisuke; Nishikawa, Kouki; Hoseki, Jun; Kitamura, Akira; Kotani, Yuri; Kiso, Kazumi; Kinjo, Masataka; Fujiyoshi, Yoshinori; Nagata, Kazuhiro

    2014-03-01

    Moyamoya disease is an idiopathic human cerebrovascular disorder that is characterized by progressive stenosis and abnormal collateral vessels. We recently identified mysterin/RNF213 as its first susceptibility gene, which encodes a 591-kDa protein containing enzymatically active P-loop ATPase and ubiquitin ligase domains and is involved in proper vascular development in zebrafish. Here we demonstrate that mysterin further contains two tandem AAA+ ATPase modules and forms huge ring-shaped oligomeric complex. AAA+ ATPases are known to generally mediate various biophysical and mechanical processes with the characteristic ring-shaped structure. Fluorescence correlation spectroscopy and biochemical evaluation suggested that mysterin dynamically changes its oligomeric forms through ATP/ADP binding and hydrolysis cycles. Thus, the moyamoya disease-associated gene product is a unique protein that functions as ubiquitin ligase and AAA+ ATPase, which possibly contributes to vascular development through mechanical processes in the cell.

  5. Characterization of South African isolates of Fusarium oxysporum f.sp cubense from Cavendish banana

    Directory of Open Access Journals (Sweden)

    Tom Gordon

    2010-04-01

    Full Text Available Fusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f.sp. cubense (Foc, is a serious vascular disease of bananas in most subtropical and tropical regions of the world. Twenty-four vegetative compatibility groups (VCGs and three pathogenic races have been identified in Foc, reflecting a relatively high genetic diversity for an asexual fungus. To characterise a South African population of Foc, a collection of 128 isolates from diverse geographic origins were isolated from diseased Cavendish bananas and subjected to VCG analysis and sequencing of the translation elongation factor 1-α (TEF gene region. The presence of mating type genes was also determined using MAT-1 and MAT-2 specific primers. VCG 0120 was established as the only VCG of Foc present in the South African population studied. Only the MAT-2 idiomorph was present in all the local isolates of Foc. A phylogenetic analysis of DNA sequences of the TEF gene region revealed that the South African isolates grouped closely with VCG 0120 isolates from Australia and Asia. These results suggest that the South African population of Foc was most likely introduced in a limited number of events and that it had spread with infected planting material within the country. The presence of only one mating type and the limited diversity in this pathogen render it unlikely to rapidly overcome disease management strategies involving host resistance.

  6. Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19

    Directory of Open Access Journals (Sweden)

    Ferrell Robert E

    2011-01-01

    Full Text Available Abstract Background Abdominal aortic aneurysm (AAA is a complex disorder with multiple genetic risk factors. Using affected relative pair linkage analysis, we previously identified an AAA susceptibility locus on chromosome 19q13. This locus has been designated as the AAA1 susceptibility locus in the Online Mendelian Inheritance in Man (OMIM database. Methods Nine candidate genes were selected from the AAA1 locus based on their function, as well as mRNA expression levels in the aorta. A sample of 394 cases and 419 controls was genotyped for 41 SNPs located in or around the selected nine candidate genes using the Illumina GoldenGate platform. Single marker and haplotype analyses were performed. Three genes (CEBPG, PEPD and CD22 were selected for DNA sequencing based on the association study results, and exonic regions were analyzed. Immunohistochemical staining of aortic tissue sections from AAA and control individuals was carried out for the CD22 and PEPD proteins with specific antibodies. Results Several SNPs were nominally associated with AAA (p CEBPG, peptidase D (PEPD, and CD22. Haplotype analysis found a nominally associated 5-SNP haplotype in the CEBPG/PEPD locus, as well as a nominally associated 2-SNP haplotype in the CD22 locus. DNA sequencing of the coding regions revealed no variation in CEBPG. Seven sequence variants were identified in PEPD, including three not present in the NCBI SNP (dbSNP database. Sequencing of all 14 exons of CD22 identified 20 sequence variants, five of which were in the coding region and six were in the 3'-untranslated region. Five variants were not present in dbSNP. Immunohistochemical staining for CD22 revealed protein expression in lymphocytes present in the aneurysmal aortic wall only and no detectable expression in control aorta. PEPD protein was expressed in fibroblasts and myofibroblasts in the media-adventitia border in both aneurysmal and non-aneurysmal tissue samples. Conclusions Association testing

  7. Learning openCV computer vision with the openCV library

    CERN Document Server

    Bradski, Gary

    2008-01-01

    Learning OpenCV puts you right in the middle of the rapidly expanding field of computer vision. Written by the creators of OpenCV, the widely used free open-source library, this book introduces you to computer vision and demonstrates how you can quickly build applications that enable computers to see" and make decisions based on the data. With this book, any developer or hobbyist can get up and running with the framework quickly, whether it's to build simple or sophisticated vision applications

  8. Simulation of bifurcated stent grafts to treat abdominal aortic aneurysms (AAA)

    CERN Document Server

    Egger, Jan; Freisleben, Bernd

    2016-01-01

    In this paper a method is introduced, to visualize bifurcated stent grafts in CT-Data. The aim is to improve therapy planning for minimal invasive treatment of abdominal aortic aneurysms (AAA). Due to precise measurement of the abdominal aortic aneurysm and exact simulation of the bifurcated stent graft, physicians are supported in choosing a suitable stent prior to an intervention. The presented method can be used to measure the dimensions of the abdominal aortic aneurysm as well as simulate a bifurcated stent graft. Both of these procedures are based on a preceding segmentation and skeletonization of the aortic, right and left iliac. Using these centerlines (aortic, right and left iliac) a bifurcated initial stent is constructed. Through the implementation of an ACM method the initial stent is fit iteratively to the vessel walls - due to the influence of external forces (distance- as well as balloonforce). Following the fitting process, the crucial values for choosing a bifurcated stent graft are measured, ...

  9. The AAA-ATPase NVL2 is a telomerase component essential for holoenzyme assembly

    Energy Technology Data Exchange (ETDEWEB)

    Her, Joonyoung [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of); Chung, In Kwon, E-mail: topoviro@yonsei.ac.kr [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of)

    2012-01-20

    Highlights: Black-Right-Pointing-Pointer Identification of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. Black-Right-Pointing-Pointer NVL2 associates with catalytically active telomerase via an interaction with hTERT. Black-Right-Pointing-Pointer NVL2 is a telomerase component essential for holoenzyme assembly. Black-Right-Pointing-Pointer ATP-binding activity of NVL2 is required for hTERT binding and telomerase assembly. -- Abstract: Continued cell proliferation requires telomerase to maintain functional telomeres that are essential for chromosome integrity. Although the core enzyme includes a telomerase reverse transcriptase (TERT) and a telomerase RNA component (TERC), a number of auxiliary proteins have been identified to regulate telomerase assembly, localization, and enzymatic activity. Here we describe the characterization of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. NVL2 interacts and co-localizes with hTERT in the nucleolus. NLV2 is also found in association with catalytically competent telomerase in cell lysates through an interaction with hTERT. Depletion of endogenous NVL2 by small interfering RNA led to a decrease in hTERT without affecting the steady-state levels of hTERT mRNA, thereby reducing telomerase activity, suggesting that NVL2 is an essential component of the telomerase holoenzyme. We also found that ATP-binding activity of NVL2 is required for hTERT binding as well as telomerase assembly. Our findings suggest that NVL2, in addition to its role in ribosome biosynthesis, is essential for telomerase biogenesis and provides an alternative approach for inhibiting telomerase activity in cancer.

  10. Health-related quality of life outcomes following elective open or endovascular AAA repair: a randomized controlled trial.

    NARCIS (Netherlands)

    Lottman, P.E.M.; Laheij, R.J.F.; Cuypers, P.W.; Bender, M.H.M.; Buth, J.

    2004-01-01

    PURPOSE: To assess health-related quality of life outcomes after endovascular versus open abdominal aortic aneurysm repair. METHODS: Participants were randomly assigned to receive either endovascular or open abdominal aortic aneurysm (AAA) surgery according to a rate of 3 endovascular patients to 1

  11. Clinical dosimetric impact of Acuros XB and analytical anisotropic algorithm (AAA on real lung cancer treatment plans : review

    Directory of Open Access Journals (Sweden)

    Suresh Rana

    2014-02-01

    Full Text Available Photon dose calculation algorithms in treatment planning system could affect the accuracy of dose delivery when tissue heterogeneity is involved along the beam path. Treatment planning for lung cancer is challenging, especially in the case of treatment plan involving small fields. The combination of low-density (air medium and small fields cause charge particle disequilibrium nears the air/tissue interface. Beam modeling within the dose calculation algorithms must also employ an accurate method of accounting tissue heterogeneity corrections in order to avoid dose overestimation or underestimation. Analytical anisotropic algorithm (AAA is one of the widely tested and validated dose calculation algorithms in external beam photon radiation therapy. Recently, Acuros XB (AXB was made available for photon dose calculations, and several studies have demonstrated better dose prediction accuracy of the AXB over AAA. This article reviews the results from the treatment planning studies, which have investigated the clinical dosimetric impact of the AXB and AAA on real lung cancer treatment plans.--------------------------------------Cite this article as: Rana S. Clinical dosimetric impact of Acuros XB and analytical anisotropic algorithm (AAA on real lung cancer treatment plans: review. Int J Cancer Ther Oncol 2014; 2(1:02019.DOI: http://dx.doi.org/10.14319/ijcto.0201.9

  12. Foot-and-Mouth Disease Virus 2C Is a Hexameric AAA+ Protein with a Coordinated ATP Hydrolysis Mechanism

    DEFF Research Database (Denmark)

    Sweeney, Trevor; Cisnetto, Valentina; Bose, Daniel;

    2010-01-01

    Foot-and-mouth disease virus (FMDV), a positive sense, single-stranded RNA virus, causes a highly contagious disease in cloven-hoofed livestock. Like other picornaviruses, FMDV has a conserved 2C protein assigned to the superfamily 3 helicases a group of AAA+ ATPases that has a predicted N...

  13. Student-Athlete Perceptions of a Summer Pre-Enrollment Experience at an NCAA Division I-AAA Institution

    Science.gov (United States)

    Dalgety, Michael Franklin

    2012-01-01

    The purpose of this exploratory qualitative study was to examine student-athlete perceptions of the role of summer pre-enrollment in their adjustment and transition to college. The study focused on student-athletes who received athletically-related financial aid at a National Collegiate Athletic Association (NCAA) Division I-AAA institution. The…

  14. Experimental examination on the heterogeneity parameter Cv of earthquake precursors*

    Institute of Scientific and Technical Information of China (English)

    王凯英; 马胜利; 刘力强; 马瑾

    2002-01-01

    Two rock samples with different structures and materials were deformed under a biaxial loading system, and multi-point strain measurements were performed for each sample. The distribution of strain anomalies during the deformation and the instability process were analyzed by using Cv value put forward by WANG Xiao-qing and CHEN Xue-zhong, et al, a parameter to describe the heterogeneous distribution of earthquake precursors, so as to examine the method of Cv value and to explore its physical meaning experimentally. The result shows that the change of Cv value is correlated to the change of deformation characteristics and is an effective parameter to describe the heterogeneity of precursor distribution. Cv value increases firstly and then decreases before the instability, and the instability occurs when Cv value decreases to the level before increasing. This indicates that Cv value may be a useful parameter for earthquake prediction.

  15. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    Energy Technology Data Exchange (ETDEWEB)

    Ottosson, R O; Karlsson, A; Behrens, C F, E-mail: riolot01@heh.regionh.d [Department of Oncology (R), Division of Radiophysics (52AA), Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK-2730 Herlev (Denmark)

    2010-08-21

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  16. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    Science.gov (United States)

    Ottosson, R. O.; Karlsson, A.; Behrens, C. F.

    2010-08-01

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  17. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    International Nuclear Information System (INIS)

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  18. Adsorption Study on Moringa Oleifera Seeds and Musa Cavendish as Natural Water Purification Agents for Removal of Lead, Nickel and Cadmium from Drinking Water

    Science.gov (United States)

    Aziz, N. A. A.; Jayasuriya, N.; Fan, L.

    2016-07-01

    The effectiveness of plant based materials Moringa oleifera (Moringa) seeds and Musa cavendish (banana peel) for removing heavy metals namely lead (Pb), nickel (Ni) and cadmium (Cd) from contaminated groundwater was studied. Tests were carried out with individual and combined biomass at neutral pH condition on synthetic groundwater samples. The optimum biomass doses were determined as 200 mg/L for single biomass and 400 mg/L (in the ratio of 200 mg/L: 200 mg/L) for combined biomasses and used for adsorption isotherm studies with contact time of 30 minutes. Results showed that combined biomasses was able to met the Pb, Ni and Cd WHO standards from higher Pb, Ni and Cd initial concentrations which were up to 40 µg/L, 50 µg/L 9 µg/L, respectively compared to individual biomass of Moringa seed and banana peel. Moringa seeds exhibited the highest removal of Pb (81%) while the combined biomasses was most effective in removing Ni (74%) and Cd (97%) over wider their initial concentration ranges. The experimental data were linearized with Langmuir and Freundlich adsorption isotherm models. Freundlich model described the Pb adsorption better than the Langmuir model for all the tested biomasses. However, the Langmuir model fit better with the experimental data of Ni adsorption by Moringa seeds. Both models showed negligible differences in the coefficient of determination (R2) when applied for Ni and Cd adsorption on banana peel and combined biomasses, suggesting that there were multiple layers on the biomass interacting with the metals. Chemisorption is suggested to be involved in Pb adsorption for all tested biomasses as the value of nF calculated was lower than one. This type of adsorption could explain the phenomenon of different behavior of Pb removal and the higher Pb adsorption capacity (represented by KF values) compared to Ni and Cd. The study demonstrates that Moringa seeds, banana peel and their combination have the potential to be used as a natural alternative

  19. Cloning and sequencing of Duck circovirus (DuCV).

    Science.gov (United States)

    Hattermann, K; Schmitt, C; Soike, D; Mankertz, A

    2003-12-01

    The genome of Duck circovirus (DuCV) is circular and 1996 nts in size. Two major open reading frames were identified, encoding the replicase (V1) and the capsid protein (C1). A stem-loop structure comprising the nonamer 5'-TATTATTAC, conserved in all circo-, nano- and geminiviruses, was found. Unique to DuCV, the region between the 3'-ends of the rep and cap gene contains four repeats of a 44-bp sequence. Phylogenetic analysis shows close relation of DuCV with Goose circovirus and suggests classification of DuCV as a new member of the genus Circovirus of the virus family Circoviridae. PMID:14648300

  20. Protective effect of CV247 against cisplatin nephrotoxicity in rats.

    Science.gov (United States)

    Máthé, C; Szénási, G; Sebestény, A; Blázovics, A; Szentmihályi, K; Hamar, P; Albert, M

    2014-08-01

    CV247 (CV), an aqueous mixture of copper (Cu) and manganese (Mn) gluconates, vitamin C and sodium salicylate increased the antitumour effects of cisplatin (CDPP; cis-diamminedichloroplatinum) in vitro. We hypothesized that the antioxidant and cyclooxygenase-2 (COX-2; prostaglandin-endoperoxide synthase 2) inhibitory components of CV can protect the kidneys from CDPP nephrotoxicity in rats. CDPP (6.5 mg/kg, intraperitoneally) slightly elevated serum creatinine (Crea) and blood urea nitrogen (BUN) 12 days after treatment. Kidney histology demonstrated extensive tubular epithelial damage and COX-2 immunoreactivity increased 14 days after treatment. A large amount of platinum (Pt) accumulated in the kidney of CDPP-treated rats. Furthermore, CDPP decreased renal iron (Fe), molybdenum (Mo), zinc (Zn), Cu and Mn concentrations and increased plasma Fe and Cu concentrations. CDPP elevated plasma free radical concentration. Treatment with CV alone for 14 days (twice 3 ml/kg/day orally) did not influence these parameters. Chronic CV administration after CDPP reduced renal histological damage and slightly decreased COX-2 immunoreactivity, while failed to prevent the increase in Crea and BUN levels. Blood free radical concentration was reduced, that is, CV improved redox homeostasis. CV restored plasma Fe and renal Fe, Mo and Zn, while decreased Pt and elevated Cu and Mn concentrations in the kidney. Besides the known synergistic antitumour effects with CDPP, CV partially protected the kidneys from CDPP nephrotoxicity probably through its antioxidant effect.

  1. AAAS Mass Media Science and Engineering Fellowship Program: Building Communication Skills in Young Scientists

    Science.gov (United States)

    Pasco, S.

    2006-12-01

    The AAAS Mass Media Science &Engineering Fellowship program has succeeded in training scientists to become more effective communicators for more than 30 years. The program places advanced science, engineering and mathematics students at media sites to work as science reporters for ten weeks each summer. AAAS places between 15 to 20 students a year at newspapers, magazines and radio stations. Our goal is to create better science communicators who understand their role in fostering the public's understanding of science. Fellows leave the program with a greater awareness of how to communicate complex issues by making the connection as to why people should be interested in certain developments, and more specifically, how they will impact their communities. 2004 AGU Fellow Rei Ueyama put her lessons learned to good use during her Fellowship at the Sacramento Bee. "In a regional paper like The Bee, a (story) also had to have a local touch. I needed to show why people in Sacramento (or California) should bother to read the story. One example is the story I wrote about seeding the ocean with iron particles to fight global warming. Since ocean fertilization is a global issue, I had to clearly specify the reason why The Bee and not The New York Times was running the story. The local angle I chose was to point out that the core group of scientists involved in this study was from Monterey Bay, Calif." Many alumni tell us the program has been an integral force in shaping the course of their career. Similarly, sites often report that having a scientist on staff is an invaluable resource that allows them to cover additional science stories as well as report some technical stories in more depth. The American Geophysical Union has sponsored a Mass Media Fellow since 1997. Sponsorship allows affiliate program partners to establish connections with young professionals in their field. They are then also able to take advantage of the communication skills resident in their alumni base

  2. Critical clamp loader processing by an essential AAA+ protease in Caulobacter crescentus.

    Science.gov (United States)

    Vass, Robert H; Chien, Peter

    2013-11-01

    Chromosome replication relies on sliding clamps that are loaded by energy-dependent complexes. In Escherichia coli, the ATP-binding clamp loader subunit DnaX exists as both long (τ) and short (γ) forms generated through programmed translational frameshifting, but the need for both forms is unclear. Here, we show that in Caulobacter crescentus, DnaX isoforms are unexpectedly generated through partial proteolysis by the AAA+ protease casein lytic proteinase (Clp) XP. We find that the normally processive ClpXP protease partially degrades DnaX to produce stable fragments upon encountering a glycine-rich region adjacent to a structured domain. Increasing the sequence complexity of this region prevents partial proteolysis and generates a τ-only form of DnaX in vivo that is unable to support viability on its own. Growth is restored when γ is provided in trans, but these strains are more sensitive to DNA damage compared with strains that can generate γ through proteolysis. Our work reveals an unexpected mode of partial processing by the ClpXP protease to generate DnaX isoforms, demonstrates that both τ and γ forms of DnaX are required for Caulobacter viability, and identifies a role for clamp loader diversity in responding to DNA damage. The conservation of distinct DnaX isoforms throughout bacteria despite fundamentally different mechanisms for producing them suggests there may be a conserved need for alternate clamp loader complexes during DNA damaging conditions.

  3. Formulation and evaluation of semisolid jelly produced by Musa acuminata Colla (AAA Group) peels

    Institute of Scientific and Technical Information of China (English)

    Noor Azwani Mohd Rasidek; Mariam Firdhaus Mad Nordin; Kamyar Shameli

    2016-01-01

    Objective: To study the jelly formulation produced by Musa acuminata Colla (AAA Group) peels and evaluate its antioxidant properties which are related to the product quality. Methods: The formulations of peel jelly were established under two-level full factorial designs within two blocks and one center point. Regarding response optimizer, the amount of sugar and citric acid was obtained; hence, the peel jellies were produced. The evaluation of antioxidant properties was conducted by using total phenolic content (TPC) assay and 1,1 diphenyl-2-picrylhydrazyl (DPPH) free radical assay. Results: The TPC of peel powder varied from 91.8 to 602.26 mg gallic acid equivalents/100 g dry weight, and 5%–7% peel jellies had phenolic content ranging from 29.38 to 48.31 mg gallic acid equivalents/100 g dry weight. The results of DPPH test indicated that at 10 mg/mL, the peel powder showed 89% DPPH inhibition, while 7% peel jelly prominently exhibited 84% DPPH inhibition. The correlation between DPPH IC50 value and TPC of peel powder as well as peel jelly was quite reasonably high with correlation coefficient ranging from 0.843 7 to 0.995. Conclusions: TPC can be used as an indicator in assessing the antioxidant activity of fruits and vegetables. The present investigation reveals that TPC is mainly responsible for DPPH free radical scavenging capacity.

  4. Simulation of bifurcated stent grafts to treat abdominal aortic aneurysms (AAA)

    Science.gov (United States)

    Egger, J.; Großkopf, S.; Freisleben, B.

    2007-03-01

    In this paper a method is introduced, to visualize bifurcated stent grafts in CT-Data. The aim is to improve therapy planning for minimal invasive treatment of abdominal aortic aneurysms (AAA). Due to precise measurement of the abdominal aortic aneurysm and exact simulation of the bifurcated stent graft, physicians are supported in choosing a suitable stent prior to an intervention. The presented method can be used to measure the dimensions of the abdominal aortic aneurysm as well as simulate a bifurcated stent graft. Both of these procedures are based on a preceding segmentation and skeletonization of the aortic, right and left iliac. Using these centerlines (aortic, right and left iliac) a bifurcated initial stent is constructed. Through the implementation of an ACM method the initial stent is fit iteratively to the vessel walls - due to the influence of external forces (distance- as well as balloonforce). Following the fitting process, the crucial values for choosing a bifurcated stent graft are measured, e.g. aortic diameter, right and left common iliac diameter, minimum diameter of distal neck. The selected stent is then simulated to the CT-Data - starting with the initial stent. It hereby becomes apparent if the dimensions of the bifurcated stent graft are exact, i.e. the fitting to the arteries was done properly and no ostium was covered.

  5. Versatile modes of peptide recognition by the AAA+ adaptor protein SspB

    Energy Technology Data Exchange (ETDEWEB)

    Levchenko, Igor; Grant, Robert A.; Flynn, Julia M.; Sauer, Robert T.; Baker, Tania A. (MIT)

    2010-07-19

    Energy-dependent proteases often rely on adaptor proteins to modulate substrate recognition. The SspB adaptor binds peptide sequences in the stress-response regulator RseA and in ssrA-tagged proteins and delivers these molecules to the AAA+ ClpXP protease for degradation. The structure of SspB bound to an ssrA peptide is known. Here, we report the crystal structure of a complex between SspB and its recognition peptide in RseA. Notably, the RseA sequence is positioned in the peptide-binding groove of SspB in a direction opposite to the ssrA peptide, the two peptides share only one common interaction with the adaptor, and the RseA interaction site is substantially larger than the overlapping ssrA site. This marked diversity in SspB recognition of different target proteins indicates that it is capable of highly flexible and dynamic substrate delivery.

  6. AAA-ATPase FIDGETIN-LIKE 1 and Helicase FANCM Antagonize Meiotic Crossovers by Distinct Mechanisms.

    Directory of Open Access Journals (Sweden)

    Chloe Girard

    2015-07-01

    Full Text Available Meiotic crossovers (COs generate genetic diversity and are critical for the correct completion of meiosis in most species. Their occurrence is tightly constrained but the mechanisms underlying this limitation remain poorly understood. Here we identified the conserved AAA-ATPase FIDGETIN-LIKE-1 (FIGL1 as a negative regulator of meiotic CO formation. We show that Arabidopsis FIGL1 limits CO formation genome-wide, that FIGL1 controls dynamics of the two conserved recombinases DMC1 and RAD51 and that FIGL1 hinders the interaction between homologous chromosomes, suggesting that FIGL1 counteracts DMC1/RAD51-mediated inter-homologue strand invasion to limit CO formation. Further, depleting both FIGL1 and the previously identified anti-CO helicase FANCM synergistically increases crossover frequency. Additionally, we showed that the effect of mutating FANCM on recombination is much lower in F1 hybrids contrasting from the phenotype of inbred lines, while figl1 mutation equally increases crossovers in both contexts. This shows that the modes of action of FIGL1 and FANCM are differently affected by genomic contexts. We propose that FIGL1 and FANCM represent two successive barriers to CO formation, one limiting strand invasion, the other disassembling D-loops to promote SDSA, which when both lifted, leads to a large increase of crossovers, without impairing meiotic progression.

  7. Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation.

    Science.gov (United States)

    Gal, Csenge; Murton, Heather E; Subramanian, Lakxmi; Whale, Alex J; Moore, Karen M; Paszkiewicz, Konrad; Codlin, Sandra; Bähler, Jürg; Creamer, Kevin M; Partridge, Janet F; Allshire, Robin C; Kent, Nicholas A; Whitehall, Simon K

    2016-01-01

    Maintenance of the correct level and organisation of nucleosomes is crucial for genome function. Here, we uncover a role for a conserved bromodomain AAA-ATPase, Abo1, in the maintenance of nucleosome architecture in fission yeast. Cells lacking abo1(+) experience both a reduction and mis-positioning of nucleosomes at transcribed sequences in addition to increased intragenic transcription, phenotypes that are hallmarks of defective chromatin re-establishment behind RNA polymerase II. Abo1 is recruited to gene sequences and associates with histone H3 and the histone chaperone FACT. Furthermore, the distribution of Abo1 on chromatin is disturbed by impaired FACT function. The role of Abo1 extends to some promoters and also to silent heterochromatin. Abo1 is recruited to pericentromeric heterochromatin independently of the HP1 ortholog, Swi6, where it enforces proper nucleosome occupancy. Consequently, loss of Abo1 alleviates silencing and causes elevated chromosome mis-segregation. We suggest that Abo1 provides a histone chaperone function that maintains nucleosome architecture genome-wide.

  8. Development and Analysis of Synthetic Composite Materials Emulating Patient AAA Wall Material Properties

    Science.gov (United States)

    Margossian, Christa M.

    Abdominal Aortic Aneurysm (AAA) rupture accounts for 14,000 deaths a year in the United States. Since the number of ruptures has not decreased significantly in recent years despite improvements in imaging and surgical procedures, there is a need for an accurate, noninvasive technique capable of establishing rupture risk for specific patients and discriminating lesions at high risk. In this project, synthetic composite materials replicating patient-specific wall stiffness and strength were developed and their material properties evaluated. Composites utilizing various fibers were developed to give a range of stiffness from 1825.75 kPa up through 8187.64 kPa with one base material, Sylgard 170. A range of strength from 631.12 kPa to 1083 kPa with the same base material was also found. By evaluating various base materials and various reinforcing fibers, a catalogue of stiffnesses and strengths was started to allow for adaptation to specific patient properties. Three specific patient properties were well-matched with two composites fabricated: silk thread-reinforced Sylgard 170 and silk thread-reinforced Dragon Skin 20. The composites showed similar stiffnesses to the specific patients while reaching target stresses at particular strains. Not all patients were matched with composites as of yet, but recommendations for future matches are able to be determined. These composites will allow for the future evaluation of flow-induced wall stresses in models replicating patient material properties and geometries.

  9. Graphite whiskers in CV3 meteorites.

    Science.gov (United States)

    Fries, Marc; Steele, Andrew

    2008-04-01

    Graphite whiskers (GWs), an allotrope of carbon that has been proposed to occur in space, have been discovered in three CV-type carbonaceous chondrites via Raman imaging and electron microscopy. The GWs are associated with high-temperature calcium-aluminum inclusion (CAI) rims and interiors, with the rim of a dark inclusion, and within an inclusion inside an unusual chondrule that bears mineralogy and texture indicative of high-temperature processing. Current understanding of CAI formation places their condensation, and that of associated GWs, relatively close to the Sun and early in the condensation sequence of protoplanetary disk materials. If this is the case, then it is a possibility that GWs are expelled from any young solar system early in its history, thus populating interstellar space with diffuse GWs. Graphite whiskers have been postulated to play a role in the near-infrared (near-IR) dimming of type Ia supernovae, as well as in the thermalization of both the cosmic IR and microwave background and in galactic center dimming between 3 and 9 micrometers. Our observations, along with the further possibility that GWs could be manufactured during supernovae, suggest that GWs may have substantial effects in observational astronomy. PMID:18309047

  10. Facial Recognition using OpenCV

    Directory of Open Access Journals (Sweden)

    Valentin Petrut Suciu

    2012-03-01

    Full Text Available

    The growing interest in computer vision of the past decade. Fueled by the steady doubling rate of computing power every 13 months, face detection and recognition has transcended from an esoteric to a popular area of research in computer vision and one of the better and successful applications of image analysis and algorithm based understanding. Because of the intrinsic nature of the problem, computer vision is not only a computer science area of research, but also the object of neuro-scientific and psychological studies, mainly because of the general opinion that advances in computer image processing and understanding research will provide insights into how our brain work and vice versa.

    Because of general curiosity and interest in the matter, the author has proposed to create an application that would allow user access to a particular machine based on an in-depth analysis of a person’s facial features. This application will be developed using Intel’s open source computer vision project, OpenCV and Microsoft’s .NET framework.

  11. Facial Recognition using OpenCV

    Directory of Open Access Journals (Sweden)

    Shervin Emami

    2012-03-01

    Full Text Available The growing interest in computer vision of the past decade. Fueled by the steady doubling rate of computing power every 13 months, face detection and recognition has transcended from an esoteric to a popular area of research in computer vision and one of the better and successful applications of image analysis and algorithm based understanding. Because of the intrinsic nature of the problem, computer vision is not only a computer science area of research, but also the object of neuro-scientific and psychological studies, mainly because of the general opinion that advances in computer image processing and understanding research will provide insights into how our brain work and vice versa. Because of general curiosity and interest in the matter, the author has proposed to create an application that would allow user access to a particular machine based on an in-depth analysis of a person’s facial features. This application will be developed using Intel’s open source computer vision project, OpenCV and Microsoft’s .NET framework.

  12. Detecting endoleaks after endovascular AAA repair with a minimally invasive, implantable, telemetric pressure sensor: an in vitro study

    Energy Technology Data Exchange (ETDEWEB)

    Springer, Fabian; Pfeffer, Joachim-Georg; Schmitz-Rode, Thomas [RWTH Aachen University, Helmholtz-Institute for Biomedical Engineering, Applied Medical Engineering, Aachen (Germany); Schlierf, Roland; Schnakenberg, Uwe [RWTH Aachen University, Institute of Materials in Electrical Engineering I, Aachen (Germany); Mahnken, Andreas H. [RWTH Aachen University, Helmholtz-Institute for Biomedical Engineering, Applied Medical Engineering, Aachen (Germany); RWTH Aachen University, Department of Diagnostic Radiology, University Hospital, Aachen (Germany)

    2007-10-15

    A feasibility study on a completely digital telemetric pressure sensor (TPS) to detect endoleaks was performed in an in vitro model of an abdominal aortic aneurysm (AAA). An endovascular-stented AAA silicone model with different types (I-III) and sizes (3-11 French) of endoleaks was created and pulsatile pressure was applied with physiological flow and pressure rates [mean intraaortic pressure (IAP): 95-130 mmHg] and different degrees of thrombosis of the aneurysm sac. Aneurysm sac pressure (ASP) was measured with the TPS and with wired pressure sensors (WPS) as a reference. Statistical analysis included paired t-test, Pearson's correlation analysis and Bland-Altman plots. After opening an endoleak, the mean ASP increased significantly (P < 0.0001) from 15 to almost 95% of the mean IAP depending on endoleak type and size. ASP could be measured accurately with the TPS and the WPS. The telemetric and wired ASP increase showed a high Pearson's correlation coefficient (r) for a non-thrombosed (r = 0.97) and a thrombosed (r = 0.96) aneurysm sac. In an in vitro silicone model, the newly designed telemetric pressure sensor was able to detect the occurrence of an endoleak in a non-invasive way and might be a valuable device for follow-up of endovascular AAA repair. (orig.)

  13. A practical introduction to computer vision with OpenCV

    CERN Document Server

    Dawson-Howe, Kenneth

    2014-01-01

    Explains the theory behind basic computer vision and provides a bridge from the theory to practical implementation using the industry standard OpenCV libraries Computer Vision is a rapidly expanding area and it is becoming progressively easier for developers to make use of this field due to the ready availability of high quality libraries (such as OpenCV 2).  This text is intended to facilitate the practical use of computer vision with the goal being to bridge the gap between the theory and the practical implementation of computer vision. The book will explain how to use the relevant OpenCV

  14. EFECTO DE DOS TIPOS DE FUNDAS SOBRE EL FRUTO DE BANANO (Musa AAA

    Directory of Open Access Journals (Sweden)

    Alfonso Vargas-Calvo

    2011-01-01

    Full Text Available El objetivo de este estudio fue determinar el efecto de dos fundas en la protección del racimo de banano (Musa AAA . En dos épocas climáticas (adversa y favorable bajo condiciones del Ca ribe de Costa Ri ca se evaluaron dos fundas: 1- azul Sa nta Lucía (bifentrina 0,1%, polie tileno de 12,7 ¿ de grosor, con perforaciones de 4 mm y 86,4 cm de ancho y 2- transparente con aditivos para filtrar la luz ultravioleta e infrarroja (bifentrina 0,1%, 20,3 ¿ de grosor, con perforaciones de 4 mm y 88,9 cm de ancho. El peso del racimo así como el grosor y la longitud del fruto central de la fila externa en la segunda, cuarta y sexta mano no difirieron (P> 0,0556 entre ambas fundas. Tampoco hubo diferencias entre estas en la apa rie ncia del racimo (P>0,4699 ni en la firmeza de la cáscara en grado 1 de maduración (P= 0,6268. En las varia bles de medición del color del fruto solamente L* presentó un valor (56,48 más alto (P=0,0109 con la funda transparente fotosensible, mie ntras que las otras dos varia bles relacionadas (a* y b* no fueron diferentes (P>0,1011 entre las fundas. El incremento adicional de 7,6 ¿ en el grosor del polie tileno de la funda transparente con respecto a la azul Sa nta Lucía, no ocasionó un incremento productivo ni una mejor apa rie ncia del racimo de banano y sus frutos.

  15. Calibration of ADRET voltage generator type CV102. Program CODAV

    International Nuclear Information System (INIS)

    The CODAV programm studied by the Metrology SES/SME laboratory is used for the calibration of ADRET voltage generator type CV.102. A JCAM.10 microcomputer run the measurement cycle and the printout of the results

  16. Individual Responsiveness to Information in CV Surveys : Commitment Matters

    OpenAIRE

    Olivier Chanel; Susan Cleary; Stéphane Luchini

    2007-01-01

    This paper enquires into the responsiveness of individuals to information in Contingent Valuation (CV). The impact of information is assessed using a sequential procedure in which individuals are successively presented with different levels of information. Two different types of information have been provided: scientific information about the good and information about the willingness to pay (WTP) values of the other respondents. Responsiveness to information is studied using an innovative CV...

  17. A dosimetric evaluation of the Eclipse AAA algorithm and Millennium 120 MLC for cranial intensity-modulated radiosurgery

    Energy Technology Data Exchange (ETDEWEB)

    Calvo Ortega, Juan Francisco, E-mail: jfcdrr@yahoo.es; Moragues, Sandra; Pozo, Miquel; José, Sol San; Puertas, Enrique; Fernández, Jaime; Casals, Joan

    2014-07-01

    The aim of this study is to assess the accuracy of a convolution-based algorithm (anisotropic analytical algorithm [AAA]) implemented in the Eclipse planning system for intensity-modulated radiosurgery (IMRS) planning of small cranial targets by using a 5-mm leaf-width multileaf collimator (MLC). Overall, 24 patient-based IMRS plans for cranial lesions of variable size (0.3 to 15.1 cc) were planned (Eclipse, AAA, version 10.0.28) using fixed field-based IMRS produced by a Varian linear accelerator equipped with a 120 MLC (5-mm width on central leaves). Plan accuracy was evaluated according to phantom-based measurements performed with radiochromic film (EBT2, ISP, Wayne, NJ). Film 2D dose distributions were performed with the FilmQA Pro software (version 2011, Ashland, OH) by using the triple-channel dosimetry method. Comparison between computed and measured 2D dose distributions was performed using the gamma method (3%/1 mm). Performance of the MLC was checked by inspection of the DynaLog files created by the linear accelerator during the delivery of each dynamic field. The absolute difference between the calculated and measured isocenter doses for all the IMRS plans was 2.5% ± 2.1%. The gamma evaluation method resulted in high average passing rates of 98.9% ± 1.4% (red channel) and 98.9% ± 1.5% (blue and green channels). DynaLog file analysis revealed a maximum root mean square error of 0.46 mm. According to our results, we conclude that the Eclipse/AAA algorithm provides accurate cranial IMRS dose distributions that may be accurately delivered by a Varian linac equipped with a Millennium 120 MLC.

  18. Anonymous voting for multi-dimensional CV quantum system

    Science.gov (United States)

    Rong-Hua, Shi; Yi, Xiao; Jin-Jing, Shi; Ying, Guo; Moon-Ho, Lee

    2016-06-01

    We investigate the design of anonymous voting protocols, CV-based binary-valued ballot and CV-based multi-valued ballot with continuous variables (CV) in a multi-dimensional quantum cryptosystem to ensure the security of voting procedure and data privacy. The quantum entangled states are employed in the continuous variable quantum system to carry the voting information and assist information transmission, which takes the advantage of the GHZ-like states in terms of improving the utilization of quantum states by decreasing the number of required quantum states. It provides a potential approach to achieve the efficient quantum anonymous voting with high transmission security, especially in large-scale votes. Project supported by the National Natural Science Foundation of China (Grant Nos. 61272495, 61379153, and 61401519), the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20130162110012), and the MEST-NRF of Korea (Grant No. 2012-002521).

  19. Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study.

    Science.gov (United States)

    Huang, Rui; Ripstein, Zev A; Augustyniak, Rafal; Lazniewski, Michal; Ginalski, Krzysztof; Kay, Lewis E; Rubinstein, John L

    2016-07-19

    The AAA+ (ATPases associated with a variety of cellular activities) enzymes play critical roles in a variety of homeostatic processes in all kingdoms of life. Valosin-containing protein-like ATPase of Thermoplasma acidophilum (VAT), the archaeal homolog of the ubiquitous AAA+ protein Cdc48/p97, functions in concert with the 20S proteasome by unfolding substrates and passing them on for degradation. Here, we present electron cryomicroscopy (cryo-EM) maps showing that VAT undergoes large conformational rearrangements during its ATP hydrolysis cycle that differ dramatically from the conformational states observed for Cdc48/p97. We validate key features of the model with biochemical and solution methyl-transverse relaxation optimized spectroscopY (TROSY) NMR experiments and suggest a mechanism for coupling the energy of nucleotide hydrolysis to substrate unfolding. These findings illustrate the unique complementarity between cryo-EM and solution NMR for studies of molecular machines, showing that the structural properties of VAT, as well as the population distributions of conformers, are similar in the frozen specimens used for cryo-EM and in the solution phase where NMR spectra are recorded. PMID:27402735

  20. The AAA+ proteins Pontin and Reptin enter adult age: From understanding their basic biology to the identification of selective inhibitors

    Directory of Open Access Journals (Sweden)

    Pedro M Matias

    2015-05-01

    Full Text Available Pontin and Reptin are related partner proteins belonging to the AAA+ (ATPases Associated with various cellular Activities family. They are implicated in multiple and seemingly unrelated processes encompassing the regulation of gene transcription, the remodeling of chromatin, DNA damage sensing and repair, and the assembly of protein and ribonucleoprotein complexes, among others. The 2nd International Workshop on Pontin and Reptin took place at the Instituto de Tecnologia Química e Biológica António Xavier in Oeiras, Portugal on October 10-12, 2014, and reported significant new advances on the mechanisms of action of these two AAA+ ATPases. The major points under discussion were related to the mechanisms through which these proteins regulate gene transcription, their roles as co-chaperones, and their involvement in pathophysiology, especially in cancer and ciliary biology and disease. Finally, they may become anticancer drug targets since small chemical inhibitors were shown to produce anti-tumor effects in animal models.

  1. The AAA+ proteins Pontin and Reptin enter adult age: from understanding their basic biology to the identification of selective inhibitors.

    Science.gov (United States)

    Matias, Pedro M; Baek, Sung Hee; Bandeiras, Tiago M; Dutta, Anindya; Houry, Walid A; Llorca, Oscar; Rosenbaum, Jean

    2015-01-01

    Pontin and Reptin are related partner proteins belonging to the AAA+ (ATPases Associated with various cellular Activities) family. They are implicated in multiple and seemingly unrelated processes encompassing the regulation of gene transcription, the remodeling of chromatin, DNA damage sensing and repair, and the assembly of protein and ribonucleoprotein complexes, among others. The 2nd International Workshop on Pontin and Reptin took place at the Instituto de Tecnologia Química e Biológica António Xavier in Oeiras, Portugal on October 10-12, 2014, and reported significant new advances on the mechanisms of action of these two AAA+ ATPases. The major points under discussion were related to the mechanisms through which these proteins regulate gene transcription, their roles as co-chaperones, and their involvement in pathophysiology, especially in cancer and ciliary biology and disease. Finally, they may become anticancer drug targets since small chemical inhibitors were shown to produce anti-tumor effects in animal models.

  2. Instant OpenCV for iOS

    CERN Document Server

    Kornyakov, Kirill

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. This book uses a very practical approach, with each recipe and their associated sample projects or examples focusing on a particular aspect of the technology.This book is intended for OpenCV developers who are interested in porting their applications to the iOS platform. Basic experience with OpenCV, computer vision, Objective C, and other iOS tools is encouraged.

  3. 卡文迪许实验室与贝尔实验室的人才方略比较与启示%Comparison of talent plan between Cavendish Labs and Bell Labs and its enlightenment

    Institute of Scientific and Technical Information of China (English)

    眭平

    2011-01-01

    英国卡文迪许实验室与美国贝尔实验室在科技创新中取得巨大成功的重要原因之一,是两大实验室都有科学的人才方略.通过对两大实验室的领军帅才遴选、科研人才选拔、人才团队建设等方面的比较研究,从中探讨出两大实验室人才方略的共性特点和差异特色,并提出启发性的思考.%Of all the vital reasons why Cavendish Labs and Bell Labs could achieve such tremendous success in the field of scientific innovation, scientific talent plan enjoyed by both big labs is one.Through the comparison and research on the selection of leaders and talents in scientific research and the construction of scientific research team.Of both two big labs, the general characters and different characteristic of the two labs’ talent plan are explored and discussed, and the inspirational meditations are put forward.

  4. The early-type close binary CV Velorum revisited

    NARCIS (Netherlands)

    Yakut, K.; Aerts, C.; Morel, T.

    2007-01-01

    Aims.Our goal was to improve the fundamental parameters of the massive close double-lined eclipsing B2.5V+B2.5V binary CV Velorum. Methods: We gathered new high-resolution échelle spectroscopy on 13 almost consecutive nights covering two orbits. We computed a simultaneous solution to all the availab

  5. Effect of mulching on melon (cv. Campero) crop coefficient

    DEFF Research Database (Denmark)

    Cerekovic, Natasa; Todorovic, Mladen; Snyder, Richard L.;

    2011-01-01

    The measurements of the main weather parameters for melon crop (cv. Campero) data were collected in 2002 at experimental meteorological station "E. Pantenelli" of Bari University and CNR-Bari near Policoro (Southern Italy) to investigate the influence of weather and management on crop growth and ...

  6. Ensacamento de frutos de pereira cv. Housui Bagging of nashi pear cv. Housui

    Directory of Open Access Journals (Sweden)

    Ivan Dagoberto Faoro

    2004-04-01

    Full Text Available Foi avaliada a qualidade de frutos de pêra japonesa cv. Housui (Pyrus pyrifolia var. culta ensacados com diferentes tipos de sacos de papel e em duas épocas: 34 e 83 dias após a florada. O ensacamento não influenciou na firmeza, no teor de sólidos solúveis totais e no peso médio dos frutos. Sacos vermelhos não induziram aumento do pH da polpa, mas o uso de sacos duplos, sacos marrons, sacos de papel kraft marrons e sacos de pipoca brancos aumentaram significativamente o pH. Ao buscar-se maior precocidade de colheita, os melhores resultados foram obtidos com o uso de sacos pequenos de papel manteiga aos 34 dias após a floração e o uso de sacos grandes duplos ou sacos grandes marrons, 83 dias após a plena floração. O ensacamento 34 dias após a plena floração, com sacos grandes de papel duplo de cor marrom ou sacos de papel kraft marrons, ou ainda o uso de sacos pequenos parafinados transparentes de papel manteiga, aos 34 dias, seguidos pela colocação, aos 83 dias, dos dois tipos de sacos grandes citados anteriormente, resultaram em frutos de melhor qualidade externa (película de coloração homogênea e mais clara, lisa e com lenticelas pouco salientes. O uso de sacos vermelhos de papel manteiga e de sacos de pipoca brancos, com ou sem ensacamento prévio com sacos pequenos de papel manteiga parafinado, não resultaram em melhoria substancial da qualidade externa do fruto.The fruit quality of Nashi pears cv. Housui (Pyrus pyrifolia var. culta have been evaluated after bagging with different paper bags at two bagging dates: 34 and 83 days after full bloom. The bagging did not affect the fruit firmness, the total soluble solids and the average fruit weight. Red bags did not affect the pH of the fruit flesh. However the fruit bagged with double bags of brown color, brown kraft paper and white popcorn paper increased the pH. In order to harvest precociously, the use of small transparent paraffin paper bags 34 days after full bloom

  7. 减少转杯纺纱单强CV%值的试验分析

    Institute of Scientific and Technical Information of China (English)

    赵博

    2005-01-01

    以纯棉纱和涤棉纱为例,就条干CV%值、捻度不匀率、捻度等因素对转杯纱单强CV%值的影响进行了试验分析,通过统计分析得出条干CV%值、百米重量CV%、捻值CV%值、毛羽CV%值等与成纱单强CV%值呈正相关,指出合理选择转杯纺的工艺参数,是降低杯纺纱单强CV%值的有效措施.

  8. 75 FR 54887 - Determination of Regulatory Review Period for Purposes of Patent Extension; REPEL-CV

    Science.gov (United States)

    2010-09-09

    ... Patent Extension; REPEL-CV AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) has determined the regulatory review period for REPEL-CV and is... in 35 U.S.C. 156(g)(3)(B). FDA recently approved for marketing the medical device, REPEL-CV....

  9. Performance Report and Improvements in CV Machine Areas

    CERN Document Server

    Monsted, A

    2000-01-01

    The performance of cooling and ventilation (CV) equipment is defined as its availability to the PS, SPS and LEP accelerators. Three teams for the accelerators plus a Utilities team, which receives performance information from the physics and technical control room, carry out the operation of this equipment. Collected performance statistics as well as data from the Computer Aided Maintenance Management system (CAMM) and supervision system provide information on the exact state of the CV equipment. This is used to more effectively schedule preventive maintenance which, by reducing system failures, results in improved equipment performance. This paper will present performance data and discuss the prospects for improving it through the use of an updated version of CAMM and a new supervision system.

  10. EN-CV during LS1: upgrade, consolidation, maintenance, operation

    CERN Document Server

    Nonis, M

    2012-01-01

    The Cooling and Ventilation (CV) Group in the Engineering Department (EN) will be heavily involved in several projects and activities during the long shutdown in 2013 and 2014 (LS1) within a timeframe limited to around twelve months. According to the requests received so far, most projects are related to the upgrade of users' equipment, consolidation work, and the construction of new plants. However, through the experience gained from the first years of the LHC run, some projects are also needed to adapt the existing installations to the new operating parameters. Some of these projects are presented hereafter, outlining the impact that they will have on operational working conditions or risks of breakdown. Finally, EN-CV activities during LS1 for maintenance, operation, and commissioning will be mentioned since they represent a major workload for the Group.

  11. CV-990 L-band SAR: A calibration experiment

    Science.gov (United States)

    Held, D. N.; Werner, C.

    1985-01-01

    Calibrated image data is required by most users of synthetic aperture radar (SAR) data particularly those attempting to classify targets based upon their radar backscatter signature as a function of frequency polarization or incidence angle. In this experiment, the backscatter derived by calibrating the NASA/JPL CV-990 L-band SAR, and the backscatter reported from a pass of the NASA/JSC C-130 scatterometer as the two instruments flew over the same site at different times are compared.

  12. Avaliação do estado nutricional de bananeiras do subgrupo Cavendish no estado de São Paulo: normas dris e níveis críticos de nutrientes Nutritional diagnosis in banana in the state of São Paulo (Brazil: dris norms and critical levels

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Junqueira Teixeira

    2007-01-01

    Full Text Available São apresentadas normas DRIS preliminares e derivados níveis críticos de nutrientes no tecido foliar (NC para bananeiras do subgrupo Cavendish, nas condições de cultivo do Estado de São Paulo. As normas DRIS e os NCs foram obtidos a partir de um banco de dados com 188 registros, relacionando produtividade e teores foliares de N, P, K, Ca, Mg, B, Cu, Fe, Mn e Zn das cultivares Grande Naine e Nanicão. Esses registros foram extraídos de experimentos de adubação realizados no Planalto Paulista e no Vale do Ribeira, em áreas irrigadas e de sequeiro, durante cinco ciclos de cultivo, variando fontes e doses de fertilizantes. Mesmo com a grande diversidade nas condições de cultivo, a relação entre o índice de balanço nutricional e a produtividade das plantas foi altamente significativa (R²=0,60; pPreliminary DRIS norms and leaf nutrient critical levels (NCL for Cavendish bananas growing in plantations of São Paulo State (Brazil are presented. DRIS norms and NCL were established from a data bank of leaf nutrient concentration (N, P, K, Ca, Mg, B, Cu, Fe, Mn and Zn and yield of banana with 188 samples. Data bank comprises information from plants of Grand Naine and Giant Cavendish growing in irrigated and non-irrigated areas, with different sources and rates of fertilizers during five crop cycles. Despite of the great diversity of cropping conditions, the regression between nutrient balance index and fruit yield was significant (R²=0.60; p<0.0001. The NCLs were derived using multiple linear regressions relating to the foliar nutrient concentration with DRIS indices of all nutrients. These values were similar to those presented in the literature, except the NCL for K which the value for local conditions was lower.

  13. Effect of Sodium Cyanide on Wheat (Triticum durum cv. Altar and T. aestivum cv. Cumhuriyet)

    DEFF Research Database (Denmark)

    Gemici, Meliha; Karshenass, A.M.; Tan, Kit

    2008-01-01

    The effect of sodium cyanide on the morphology of stem, leaves and grain yields of Triticum durum cv. Altar and Triticum aestivum cv. Cumhuriyet grown under glass was studied. Seeds were planted in six different sets of pots containing ordinary garden soil. After formation of the first leaves, th...

  14. In-situ monitoring of blood glucose level for dialysis machine by AAA-battery-size ATR Fourier spectroscopy

    Science.gov (United States)

    Hosono, Satsuki; Sato, Shun; Ishida, Akane; Suzuki, Yo; Inohara, Daichi; Nogo, Kosuke; Abeygunawardhana, Pradeep K.; Suzuki, Satoru; Nishiyama, Akira; Wada, Kenji; Ishimaru, Ichiro

    2015-07-01

    For blood glucose level measurement of dialysis machines, we proposed AAA-battery-size ATR (Attenuated total reflection) Fourier spectroscopy in middle infrared light region. The proposed one-shot Fourier spectroscopic imaging is a near-common path and spatial phase-shift interferometer with high time resolution. Because numerous number of spectral data that is 60 (= camera frame rare e.g. 60[Hz]) multiplied by pixel number could be obtained in 1[sec.], statistical-averaging improvement realize high-accurate spectral measurement. We evaluated the quantitative accuracy of our proposed method for measuring glucose concentration in near-infrared light region with liquid cells. We confirmed that absorbance at 1600[nm] had high correlations with glucose concentrations (correlation coefficient: 0.92). But to measure whole-blood, complex light phenomenon caused from red blood cells, that is scattering and multiple reflection or so, deteriorate spectral data. Thus, we also proposed the ultrasound-assisted spectroscopic imaging that traps particles at standing-wave node. Thus, if ATR prism is oscillated mechanically, anti-node area is generated around evanescent light field on prism surface. By elimination complex light phenomenon of red blood cells, glucose concentration in whole-blood will be quantify with high accuracy. In this report, we successfully trapped red blood cells in normal saline solution with ultrasonic standing wave (frequency: 2[MHz]).

  15. The Pch2 AAA+ ATPase promotes phosphorylation of the Hop1 meiotic checkpoint adaptor in response to synaptonemal complex defects

    Science.gov (United States)

    Herruzo, Esther; Ontoso, David; González-Arranz, Sara; Cavero, Santiago; Lechuga, Ana; San-Segundo, Pedro A.

    2016-01-01

    Meiotic cells possess surveillance mechanisms that monitor critical events such as recombination and chromosome synapsis. Meiotic defects resulting from the absence of the synaptonemal complex component Zip1 activate a meiosis-specific checkpoint network resulting in delayed or arrested meiotic progression. Pch2 is an evolutionarily conserved AAA+ ATPase required for the checkpoint-induced meiotic block in the zip1 mutant, where Pch2 is only detectable at the ribosomal DNA array (nucleolus). We describe here that high levels of the Hop1 protein, a checkpoint adaptor that localizes to chromosome axes, suppress the checkpoint defect of a zip1 pch2 mutant restoring Mek1 activity and meiotic cell cycle delay. We demonstrate that the critical role of Pch2 in this synapsis checkpoint is to sustain Mec1-dependent phosphorylation of Hop1 at threonine 318. We also show that the ATPase activity of Pch2 is essential for its checkpoint function and that ATP binding to Pch2 is required for its localization. Previous work has shown that Pch2 negatively regulates Hop1 chromosome abundance during unchallenged meiosis. Based on our results, we propose that, under checkpoint-inducing conditions, Pch2 also possesses a positive action on Hop1 promoting its phosphorylation and its proper distribution on unsynapsed chromosome axes. PMID:27257060

  16. Thyroid hormone receptor interacting protein 13 (TRIP13) AAA-ATPase is a novel mitotic checkpoint-silencing protein.

    Science.gov (United States)

    Wang, Kexi; Sturt-Gillespie, Brianne; Hittle, James C; Macdonald, Dawn; Chan, Gordon K; Yen, Tim J; Liu, Song-Tao

    2014-08-22

    The mitotic checkpoint (or spindle assembly checkpoint) is a fail-safe mechanism to prevent chromosome missegregation by delaying anaphase onset in the presence of defective kinetochore-microtubule attachment. The target of the checkpoint is the E3 ubiquitin ligase anaphase-promoting complex/cyclosome. Once all chromosomes are properly attached and bioriented at the metaphase plate, the checkpoint needs to be silenced. Previously, we and others have reported that TRIP13 AAA-ATPase binds to the mitotic checkpoint-silencing protein p31(comet). Here we show that endogenous TRIP13 localizes to kinetochores. TRIP13 knockdown delays metaphase-to-anaphase transition. The delay is caused by prolonged presence of the effector for the checkpoint, the mitotic checkpoint complex, and its association and inhibition of the anaphase-promoting complex/cyclosome. These results suggest that TRIP13 is a novel mitotic checkpoint-silencing protein. The ATPase activity of TRIP13 is essential for its checkpoint function, and interference with TRIP13 abolished p31(comet)-mediated mitotic checkpoint silencing. TRIP13 overexpression is a hallmark of cancer cells showing chromosomal instability, particularly in certain breast cancers with poor prognosis. We suggest that premature mitotic checkpoint silencing triggered by TRIP13 overexpression may promote cancer development.

  17. CV10_28 Irish Sea cruise report

    OpenAIRE

    O'Reilly, Shane; Szpak, Michael; Monteys, Xavier; Kelleher, Brian

    2010-01-01

    Cruise CV10_28 mobilized and demobilized from Howth, Co. Dublin, on the 2nd and 7th June 2010 respectively, and totalled over 112 operational hours. The objectives of this cruise were primarily to investigate and sample previously identified methane-related seepage structures in the Irish Designated Seabed Zone (IDSZ) of the Irish Sea, to carry out sampling grids of Dublin Bay and of the region of the IDSZ from Dublin Bay to Dundalk Bay for multidisciplinary geochemical and microbiological st...

  18. OpenCV 3.0 computer vision with Java

    CERN Document Server

    Baggio, Daniel Lélis

    2015-01-01

    If you are a Java developer, student, researcher, or hobbyist wanting to create computer vision applications in Java then this book is for you. If you are an experienced C/C++ developer who is used to working with OpenCV, you will also find this book very useful for migrating your applications to Java. All you need is basic knowledge of Java, with no prior understanding of computer vision required, as this book will give you clear explanations and examples of the basics.

  19. Quantum corrections to ward identities of chronological AVV- and AAA-current correlators for nondegenerate many-fermion systems in the four-dimensional world

    International Nuclear Information System (INIS)

    The explicit form of nontrivial quantum corrections to Ward identities for AVV- and AAA-current correlators in the four-dimensional world for nondegenerate many-fermion systems of general type is obtained. The characteristics of all nontrivial quantum corrections for nondegenerate two-flavour fermion systems are classified and described. In particular, the well-known results follow from ours for the trivial quantum corrections (anomalies) in the case of the degenerate spectrum of fermion masses

  20. Elements in nucleotide sensing and hydrolysis of the AAA+ disaggregation machine ClpB: a structure-based mechanistic dissection of a molecular motor

    Energy Technology Data Exchange (ETDEWEB)

    Zeymer, Cathleen, E-mail: cathleen.zeymer@mpimf-heidelberg.mpg.de; Barends, Thomas R. M.; Werbeck, Nicolas D.; Schlichting, Ilme; Reinstein, Jochen, E-mail: cathleen.zeymer@mpimf-heidelberg.mpg.de [Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg (Germany)

    2014-02-01

    High-resolution crystal structures together with mutational analysis and transient kinetics experiments were utilized to understand nucleotide sensing and the regulation of the ATPase cycle in an AAA+ molecular motor. ATPases of the AAA+ superfamily are large oligomeric molecular machines that remodel their substrates by converting the energy from ATP hydrolysis into mechanical force. This study focuses on the molecular chaperone ClpB, the bacterial homologue of Hsp104, which reactivates aggregated proteins under cellular stress conditions. Based on high-resolution crystal structures in different nucleotide states, mutational analysis and nucleotide-binding kinetics experiments, the ATPase cycle of the C-terminal nucleotide-binding domain (NBD2), one of the motor subunits of this AAA+ disaggregation machine, is dissected mechanistically. The results provide insights into nucleotide sensing, explaining how the conserved sensor 2 motif contributes to the discrimination between ADP and ATP binding. Furthermore, the role of a conserved active-site arginine (Arg621), which controls binding of the essential Mg{sup 2+} ion, is described. Finally, a hypothesis is presented as to how the ATPase activity is regulated by a conformational switch that involves the essential Walker A lysine. In the proposed model, an unusual side-chain conformation of this highly conserved residue stabilizes a catalytically inactive state, thereby avoiding unnecessary ATP hydrolysis.

  1. Perancangan Kemasan Transportasi Buah Jambu Air (Syzygium aqueum cv Camplong

    Directory of Open Access Journals (Sweden)

    Iswahyudi

    2015-04-01

    Full Text Available Jamboo cv Camplong was an exotic fruit from Sampang Indonesia which had high economic values. The quality of fresh Jamboo was greatly influenced by the types of packaging and ways of its transportation that affected its shelf life. The purpose of this research were designing a primary packaging and analyzing the quality of Jamboo cv Camplong after short transportation (from Sampang to Surabaya. Farmers used conventional packaging with capacity of 8.4 kg/box (dimension 478 mm x 146 mm x 354 mm. Based on theoretical packaging design showed that the flute BC cardboard (capacity 4.5 kg/box, dimension 357 mm x 217 mm x 216 mm with partition flute A cardboard. In fact, there was needed modification of the dimension (342 mm x 210 mm x 200 mm because of the fruits diameter (60-65 mm. This result did not change the efficiency usage of transportation space (91-95% and compression strength of box can support (7 boxes/ stack. The result showed that the mechanical damage after transportation were 20.87% for conventional packaging (as control and 7.70% for modification packaging design (packaging with partition.

  2. Production Management in SME's Industry: Case Study of CV Wiracana

    Directory of Open Access Journals (Sweden)

    Apriani Kurnia Suci

    2015-08-01

    Full Text Available Production Management is the act of designing, operating, and improving the productive systems – a system for getting the work done. Along the process, the synergy in between machineries, facilities and people could empower the sustainable of the production. In fact, for some cases, the implementation of unique system is needed for the production process. The example for this transformation production management is in the case of CV Wiracana, a handmade manufacturing company for folding hand fans from Bali. CV Wiracana's products are very unique, combined from mass production for the speed and an art for the custom made product. At one side, the market forces them to speed up the production and for this purpose, they must set up the new system on their production line. On the other side, the masterpiece also needs to be produced without jeopardizing mass production line schedule. The transformation production system needs to be done no later than 2015 as the urgency to fulfill the customer demand, business growth, compete in the industry and sustainability. The changes are expected to improve the production at least about 20% or doubled from the current production.

  3. The effect of emphatic stress on CV coarticulation

    Science.gov (United States)

    Modarresi, Golnaz; Sussman, Harvey M.

    2001-05-01

    The effect of emphatic stress on CV coarticulation was investigated in the speech of one male and one female native speaker of American English using locus equation slope as a measure of CV coarticulation. Stressed real word C1V2C2 tokens where C1=/b,d,g/ and V2=/i, I, e, ɛ, æ, u, o, squflg, a/ were put in carrier sentences with the, thirty, or two preceding the test word. Each sentence was read three times in a normal manner and three times with emphasis on the test token. This resulted in a total of 486 tokens per speaker (3 stop consonants * 3 V1 contexts * 9 V2 contexts *2 emphasis patterns *3 repetitions). Locus equation slopes were derived by plotting F2 onset of C1 against V2 F2 mid-vowel frequency and fitting a regression line to data points. Consonant closure duration, V2 duration, F0, and amplitude were also measured. Despite a significant increase in the acoustic correlates of emphasis, locus equation slopes remained constant as a function of emphasis and varied as a function of place of articulation. This study provides further evidence of the stability of locus equation slopes as phonetic descriptors of stop place of articulation. [Work supported by NIH.

  4. Evaluation of the Analytical Anisotropic Algorithm (AAA) in dose calculation for fields with non-uniform fluences considering heterogeneity correction; Avaliacao do Algoritmo Analitico Anisotropico (AAA) no calculo de dose para campos com fluencia nao uniforme considerando correcao de heterogeneidade

    Energy Technology Data Exchange (ETDEWEB)

    Bornatto, P.; Funchal, M.; Bruning, F.; Toledo, H.; Lyra, J.; Fernandes, T.; Toledo, F.; Marciao, C., E-mail: pricila_bornatto@yahoo.com.br [Hospital Erasto Gaertner (LPCC), Curitiba, PR (Brazil). Departamento de Radioterapia

    2014-08-15

    The purpose of this study is to evaluate the calculation of dose distribution AAA (Varian Medical Systems) for fields with non-uniform fluences considering heterogeneity correction. Five different phantoms were used with different density materials. These phantoms were scanned in the CT BrightSpeed (©GE Healthcare) upon the array of detectors MAPCHECK2 TM (Sun Nuclear Corporation) and irradiated in a linear accelerator 600 CD (Varian Medical Systems) 6MV and rate dose 400MU/min with isocentric setup. The fluences used were exported from IMRT plans, calculated by ECLIPSE™ planning system (Varian Medical Systems), and a 10x10 cm{sup 2} field to assess the heterogeneity correction for uniform fluence. The measured dose distribution was compared to the calculated by Gamma analysis with approval criteria of 3% / 3 mm and 10% threshold. The evaluation was performed using the software SNCPatient (Sun Nuclear Corporation) and considering absolute dose normalized at maximum. The phantoms best performers were those with low density materials, with an average of 99.2% approval. Already phantoms with plates of higher density material presented various fluences below 95% of the points approved. The average value reached 94.3%. It was observed a dependency between fluency and approved percentage points, whereas for the same fluency, 100% of the points have been approved in all phantoms. The approval criteria for IMRT plans recommended in most centers is 3% / 3mm with at least 95% of points approved, it can be concluded that, under these conditions, the IMRT plans with heterogeneity correction can be performed , however the quality control must be careful because the difficulty of the system to accurately predict the dose distribution in certain situations. (author)

  5. ÍNDICE CLIMÁTICO DE CRESCIMENTO PARA OS CAPINS Brachiaria brizantha cv. MARANDU, Cynodon dactylon cv. TIFTON 85 E Panicum maximum cv. TANZÂNIA E RELAÇÃO COM A PRODUÇÃO DE MASSA SECA CLIMATIC GROWTH INDEX FOR THE GRASSES Brachiaria brizantha cv. MARANDU, Cynodon dactylon cv. TIFTON 85 AND Panicum maximum CV. TANZÂNIA AND ITS RELATION TO DRY MASS PRODUCTION

    OpenAIRE

    Yngrid Loyola Franco; Marcos Welber Ribeiroda Silva; Modesto Antonio Chaves; Rilvaynia Dantas Soares; Flaviano Gomes dos Santos; Bianca Damasceno Pinho

    2008-01-01

    Este trabalho teve por objetivo estimar o índice climático de crescimento dos capins Brachiaria brizantha cv. Marandu, Cynodon dactylon cv. Tifton 85 e Panicum maximum cv. Tanzânia e correlacioná-lo com a produção de matéria seca. Realizou-se o experimento no período de novembro de 2005 a janeiro de 2006 na Universidade Estadual do Sudoeste da Bahia (UESB) em Itapetinga, BA, (15º 09’ 07"; sul, ...

  6. CV-6209, a highly potent antagonist of platelet activating factor in vitro and in vivo.

    Science.gov (United States)

    Terashita, Z; Imura, Y; Takatani, M; Tsushima, S; Nishikawa, K

    1987-07-01

    2-[N-acetyl-N-(2-methoxy-3-octadecylcarbamoyloxypropoxycarbonyl) aminomethyl]-1-ethylpyridinium chloride (CV-6209) inhibited aggregation of rabbit and human platelets induced by platelet activating factor (PAF) with the IC50 values of 7.5 X 10(-8) and 1.7 X 10(-7) M, respectively, and had little effects on the aggregation induced by arachidonic acid, ADP and collagen. The inhibitory effect of CV-6209 on the PAF-induced rabbit platelet aggregation was 104, 9, 8 and 3 times more potent than the PAF antagonists CV-3988, ONO-6240, Ginkgolide B and etizolam, respectively. CV-6209 inhibited [3H]serotonin release from rabbit platelets stimulated with PAF (3 X 10(-8) M) with a similar potency as the inhibition on the platelet aggregation. CV-6209 inhibited PAF (0.3 microgram/kg i.v.)-induced hypotension in rats (ED50, 0.009 mg/kg i.v.) with no effect on the hypotension induced by arachidonic acid, histamine, bradykinin and isoproterenol. CV-6209 (1 mg/kg) inhibited slightly the acetylcholine-induced hypotension. In rats, post-treatment with CV-6209 reversed the PAF (1 microgram/kg i.v.)-induced hypotension rapidly (ED50, 0.0046 mg/kg i.v.); CV-6209 was 74, 20, 185 and over 2100 times more potent than CV-3988, ONO-6240, Ginkgolide B and etizolam, respectively. Thus, the relative potency of the anti-PAF action of PAF analog (CV-6209, CV-3988 and ONO-6240) differed little between the inhibition of PAF-induced platelet aggregation and the reversal of PAF-induced hypotension, but that of nonPAF analogs (Ginkgolide B and etizolam) differed greatly with these assay systems, when standardized with CV-6209.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:3612533

  7. Pengaruh Program Keselamatan, Kesehatan, Dan Keamanan Kerja Terhadap Kinerja Karyawan Pada Cv. Nurina Medan

    OpenAIRE

    Kurnia, Wilya Sagita

    2016-01-01

    The aim of this research was to identify and analyze the influence of Program Safety, Health, and Safety Work on Employee Performance CV. Nurina Medan. The population in this study were employees of CV. Nurina Medan. The sampling method was purposive sampling, in which the samples were chosen with based on certain criteria. The sample criteria in this research was the student must a sample of workers who still work in progress of a project at CV. Nurina Medan. Hypothesis testing used descript...

  8. Evaluation of the Analytical Anisotropic Algorithm (AAA) in dose calculation for fields with non-uniform fluences considering heterogeneity correction

    International Nuclear Information System (INIS)

    The purpose of this study is to evaluate the calculation of dose distribution AAA (Varian Medical Systems) for fields with non-uniform fluences considering heterogeneity correction. Five different phantoms were used with different density materials. These phantoms were scanned in the CT BrightSpeed (©GE Healthcare) upon the array of detectors MAPCHECK2 TM (Sun Nuclear Corporation) and irradiated in a linear accelerator 600 CD (Varian Medical Systems) 6MV and rate dose 400MU/min with isocentric setup. The fluences used were exported from IMRT plans, calculated by ECLIPSE™ planning system (Varian Medical Systems), and a 10x10 cm2 field to assess the heterogeneity correction for uniform fluence. The measured dose distribution was compared to the calculated by Gamma analysis with approval criteria of 3% / 3 mm and 10% threshold. The evaluation was performed using the software SNCPatient (Sun Nuclear Corporation) and considering absolute dose normalized at maximum. The phantoms best performers were those with low density materials, with an average of 99.2% approval. Already phantoms with plates of higher density material presented various fluences below 95% of the points approved. The average value reached 94.3%. It was observed a dependency between fluency and approved percentage points, whereas for the same fluency, 100% of the points have been approved in all phantoms. The approval criteria for IMRT plans recommended in most centers is 3% / 3mm with at least 95% of points approved, it can be concluded that, under these conditions, the IMRT plans with heterogeneity correction can be performed , however the quality control must be careful because the difficulty of the system to accurately predict the dose distribution in certain situations. (author)

  9. Pengaruh Budaya Organisasi, Kepemimpinan, dan Motivasi Kerja terhadap Kepuasan Kerja Karyawan CV Surya Raya

    Directory of Open Access Journals (Sweden)

    Laksmi Sito Dwi Irvianti

    2012-05-01

    Full Text Available This research was conducted on CV Surya Raya which is engaged in car shipping. The aim of this study is to determine the influence of organization culture, leadership, and working motivation partially to employees’ satisfaction in CV Surya Raya. The data was collected by conducting interviews with the company and distributing questionnaires to 67 employees as respondents and then analyzed using linear regression analysis method. From the research results, leadership variable does not have a significant impact on employees' job satisfaction in CV Surya Raya. Whereas, the motivation variable has greater influence on job satisfaction of CV Surya Raya employees compared to the organization's culture.

  10. So you want to be a specialist registrar?--What to put in your CV.

    Science.gov (United States)

    Ellis, P E; Ellis, S G S; O'Brien, K D; Joshi, R I

    2002-02-01

    Dentists applying to a specialist training programme often receive conflicting advice over what to put in their curriculum vitae (CV). We conducted a survey of the Training Programme Directors of the dental specialties to determine what aspects of CV content and presentation styles are considered important. This has allowed us to construct guidelines for what to put in a CV. Recently, structured application forms have become increasingly popular and may be a more objective way to carry out the shortlisting process. The guidelines presented could also be used as a framework for medical personnel departments if structured application forms eventually replace the CV. PMID:11865819

  11. Biofuels from the Fresh Water Microalgae Chlorella vulgaris (FWM-CV) for Diesel Engines

    OpenAIRE

    Saddam H. Al-lwayzy; Talal Yusaf; Raed A. Al-Juboori

    2014-01-01

    This work aims to investigate biofuels for diesel engines produced on a lab-scale from the fresh water microalgae Chlorella vulgaris (FWM-CV). The impact of growing conditions on the properties of biodiesel produced from FWM-CV was evaluated. The properties of FWM-CV biodiesel were found to be within the ASTM standards for biodiesel. Due to the limited amount of biodiesel produced on the lab-scale, the biomass of dry cells of FWM-CV was used to yield emulsified water fuel. The preparation of...

  12. Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons.

    Science.gov (United States)

    Mankertz, A; Hattermann, K; Ehlers, B; Soike, D

    2000-01-01

    The complete nucleotide sequence of columbid circovirus (CoCV) isolated from pigeons is described. CoCV was amplified using a consensus primer PCR approach directed against conserved sequences within the rep genes of vertebrate circoviruses. The genome of CoCV is circular and 2037 nt in size. It displays 55% homology to the genome of psittacine beak and feather disease virus and is more distantly related (circo-, nano- and geminiviruses. Phylogenetic analyses suggest classification of CoCV as member of the genus Circovirus of the virus family Circoviridae. PMID:11205099

  13. CV activities on the LHC complex during the long shutdown

    CERN Document Server

    Deleval, S; Body, Y; Obrecht, M; Moccia, S; Peon, G

    2011-01-01

    The presentation gives an overview of the major projects and work foreseen to be performed during next long shutdown on cooling and ventilation plants. Several projects are needed following the experience of the last years when LHC was running, in particular the modifications in the water cooling circuits presently in overflow. Some other projects are linked to the CV consolidation plan. Finally, most of the work shall be done to respond to additional requests: SR buildings air conditioning, the need to be able to clean and maintain the LHC cooling towers without a complete stop of cooling circuits, the upgrade of the air conditioning of the CCC rack room cooling etc. For all these activities, the author will detail constraints and the impact on the schedule and on the operation of the plants that will however need to run for most of the shutdown duration. The consequence of postponing the long shutdown from 2012 to 2013 will be also covered.

  14. Cytotoxic diterpenoids from Jatropha curcas cv. nigroviensrugosus CY Yang Roots.

    Science.gov (United States)

    Liu, JieQing; Yang, YuanFeng; Xia, JianJun; Li, XuYang; Li, ZhongRong; Zhou, Lin; Qiu, MingHua

    2015-09-01

    An investigation of phytochemicals from the roots of Jatropha curcas cv. nigroviensrugosus resulted in the isolation of twenty diterpenoids, including lathyranlactone, an unusual diterpenoid lactone possessing a 5/13/3 tricyclic skeleton, jatrocurcasenones A-E and jatrophodiones B-E, as well as 10 known analogues. All isolates were evaluated for cytotoxicity against the HL-60, SMMC-772, A-549, MCF-7 and SW480 human tumor cell lines using the MTS viability assay. Four of the known analogues showed cytotoxic activity in these cell lines, with IC50 values ranging from 2.0 to 23.0 μM. Moreover, the assessment of their cytotoxic structure-activity relationships showed the epoxy ring between C-5 and C-6 and the hydroxyl group at C-2 were the key functionalities for cytotoxicity. PMID:26209936

  15. Herbs Recognition Based on Android using OpenCV

    Directory of Open Access Journals (Sweden)

    I Wayan Agus Suryawibawa

    2015-01-01

    Full Text Available Herbs are used in traditional medicine. There are so many herbs are spread across the world, it is difficult to memorize it all. This paper describes an android application to recognize herbs by their leaf characteristics (shape, veins, and keypoints. Shape and veins of leaves are recognized by Invariant Moment Method as the feature extraction. City Block Distance used to calculate the distance between the features. Whereas for detection and keypoints extraction using Oriented FAST and Rotated BRIEF on OpenCV library. This keypoints distance calculation using Brute-Force Hamming. Matching is done by calculating the shortest distance between test image and reference image. If the result is less than or equal to threshold then image is match. Experiment result show this application can achieve 79% of success rate by using keypoints. This result is influenced by glossy leaf surface, so there is many reflected light that become noise.

  16. Production of short-lived radiopharmaceuticals with CV-28 cyclotron

    International Nuclear Information System (INIS)

    A variable energy isochronous cyclotron CV-28 at the Physical Department of the Instituto de Engenharia Nuclear in Rio de Janeiro, Brazil, is used for radionuclide production of medical interest. The production methods of 67Ga, 77Br, 111In, 123I, 201Tb and their corresponding radiopharmaceuticals were developed. The radiopharmaceuticals 77Br-bromophenol, 77Br-rose bengal, 123I-hippuric acid, 123I-rose bengal, 111In-EDTA, 111In-DTPA and 111In-citrate were under routine production. Their labelling yields were 96%, 82%, 96%, 82%, 89+-6%, 92+-4% and 100+-25%, respectively. The labelling yield and purity were determined using thin layer and paper chromatography. Bio-distribution studies in experimental animals have shown the good quality of these compounds

  17. Architecture, algorithm and application of OpenCV VS%OpenCV VS架构、算法及应用

    Institute of Scientific and Technical Information of China (English)

    梁华; 李兴福

    2012-01-01

    Intel OpenCV VS (video surveillance) is an efficient and universal open source platform for video object detection, tracking and trajectory analysis. However research and application are rare due to lack of material about it. The architecture and algorithm of VS platform are dissected based on analysis of OpenCV VS source code. The algorithm description and corresponding interface are presented. A high-efficiency pedestrian statistics system based on OpenCV VS architecture is provided in this paper.%Intel OpenCV VS是OpenCV内嵌的一个高效、通用的视频目标检测、跟踪和轨迹分析的开源平台,但由于没有相关资料而缺少研究和应用.在深入挖掘OpenCV VS源码的基础上,剖析了该平台的整体架构和算法体系,给出了算法描述和相应接口.在此基础上,开发了一个基于OpenCV VS架构的稳定、高效的行人统计系统.

  18. In vitro organogenesis of zucchini squash cv. Caserta Organogênese in vitro de abobrinha-de-moita cv. Caserta

    Directory of Open Access Journals (Sweden)

    Liliane Cristina L Stipp

    2012-06-01

    Full Text Available A protocol for the in vitro culture of Cucurbita pepo cv. Caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. First, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in MS basal medium supplemented with benzylaminopurine (BAP, 4.5 µM, under a 16-h photoperiod at 27ºC. Based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in MS basal medium supplemented with different concentrations of BAP (0, 1.1, 2.2, 3.3, 4.5, or 5.5 µM and incubated under a 16-h photoperiod at 27ºC. In vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium supplemented with 4.5 µM of BAP. After 4 weeks of incubation the development of adventitious buds at the cotyledon/hypocotyl junction could be observed. These buds were transferred to elongation and rooting medium and the developed plants were acclimatized to greenhouse conditions. The morphogenic process was characterized using light and scanning electron microscopy analyses to confirm the organogenesis. The results showed that this alternate explant is efficient for in vitro culture of zucchini squash cv. Caserta. The protocol will be further examined for future use in genetic transformation experiments in this species.O objetivo do trabalho foi estudar um protocolo para o cultivo in vitro de Cucurbita pepo cv. Caserta utilizando como explante um segmento de cotilédone associado a um fragmento do hipocótilo. Explantes foram coletados de plântulas germinadas in vitro com 4 a 6 dias de idade, cultivados em meio de cultura basal MS suplementado com benzilaminopurina (BAP, 4,5 µM, e incubados sob fotoperíodo de 16 h, a 27ºC. Com base nos resultados obtidos, explantes coletados de plântulas germinadas in vitro com 4 dias de idade foram cultivados em meio de cultura MS, suplementados com

  19. New AAAS committee

    CERN Multimedia

    1971-01-01

    Dr Glen Seaborg, chairman of the US Atomic Energy Commission, has been made president elect of the American Association for the Advancement of science. He defeated two other candidates despite fears of some members that his connection with the AEC could lead to a conflict of interests (2 paragraphs).

  20. The Whipple Museum and Cavendish Laboratory, Cambridge

    Science.gov (United States)

    Pippard, Brian

    The Whipple Museum is part of the History and Philosophy of Science Department in the University of Cambridge. It is on your right as soon as you enter Free School Lane from Pembroke Street, and is normally open between 1:30 and 4:30 P.M. on weekdays. The main room, a hall with hammer-beam roof, is a relic of Stephen Perse’s school (1624) now flourishing elsewhere in the city. It houses a large collection of mathematical, physical and astronomical instruments — abaci, Napier’s bones, slide rules; sextants and other surveying instruments; telescopes, compasses and pocket sundials (especially of ivory from Nuremberg 1500-1700); and a Grand Orrery by George Adams (1750). The gallery of a second room is used for special exhibitions, often of items from the well-stocked store. Some specialist catalogues have been compiled and are on sale.

  1. PERUMUSAN STRATEGI BERSAING JAHE INSTAN PRODUK CV. INTRAFOOD SURAKARTA MENGGUNAKAN PERCEPTUAL MAPPING

    Directory of Open Access Journals (Sweden)

    Mohd. Harisudin

    2013-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui posisi bersaing jahe instan produk CV. Intrafood dan strategi bersaing yang dapat direkomendasikan untuk meraih keberasilannya. Metode dasar yang digunakan adalah analisis deskriptif. Penentuan lokasi penelitian ditentukan dengan metode purposive, yaitu CV. Intrafood Surakarta. Jenis data yang digunakan dalam penelitian ini adalah data primer dan data sekunder. Metode analisis data menggunakan analisis Perceptual Mapping. Dari hasil penelitian diperoleh informasi bahwa jahe instan produk CV Intrafood berada pada peringkat ke-2 dari produk empat jahe instan yang diperbandingkan. Atribut yang dapat dijadikan kekuatan utama dalam meningkatkan pemasaran jahe instan produk CV. Intrafood adalah manfaat produk. Sisi yang paling lemah adalah atribut desain kemasan dan kinerja produk dalam kemasan. Abstract This study aims to determine the competitive position of the product instant ginger CV. Intrafood and competitive strategy can be recommended to achieve success. The basic method used is descriptive analysis. Determining the location of the study are determined by purposive method, namely CV. Intrafood Surakarta. Data used in this study is primary data and secondary data. Method analyzed using Perceptual Mapping. From the results of the study concluded that instant ginger of CV Intrafood product ranks second of four instant ginger products are compared. Attributes that can be used as a major force in improving product marketing instant ginger CV. Intrafood is the benefits of the product. The weakest side is the packaging design and performance attributes of the product in the packaging.

  2. CV-Online tahab Londoni börsile / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Personaliotsinguga tegelev firma tahab tulevikus minna Londoni ja OMXi börsile. Diagramm: CV-Online Group loodab tänavu esmakordselt positiivse kulumieelse ärikasumi. Vt. samas: Üks CV-Online'i aktsionäridest on ka Google'i aktsionär; Väsinud ootamast esimest IPOt

  3. The maize AAA-type protein SKD1 confers enhanced salt and drought stress tolerance in transgenic tobacco by interacting with Lyst-interacting protein 5.

    Directory of Open Access Journals (Sweden)

    Zongliang Xia

    Full Text Available ATPase associated with various cellular activities (AAA proteins are important regulators involved in diverse cellular functions. To date, the molecular mechanisms of AAA proteins involved in response to salt and drought stresses in plants are largely unknown. In this study, a putative SKD1 (suppressor of K(+ transport growth defect 1 ortholog from Zea mays (ZmSKD1, which encodes a putative AAA protein, was isolated. The transcript levels of ZmSKD1 were higher in aerial tissues and were markedly up-regulated by salt or drought stress. Over-expression of ZmSKD1 in tobacco plants enhanced their tolerances not only to salt but to drought. Moreover, reactive oxygen species accumulations in ZmSKD1 transgenic lines were relative less than those in wild-type plants during salt or PEG-induced water stress. The interaction between ZmSKD1 and NtLIP5 (Lyst-Interacting Protein 5 homolog from Nicotiana tabacum was confirmed by both yeast two-hybrid and immuno-precipitation assays; moreover, the α-helix-rich domain in the C-terminus of ZmSKD1 was identified to be required for its interaction with NtLIP5 using truncation mutations. Collectively, these data demonstrate that ZmSKD1could be involved in salt and drought stress responses and its over-expression enhances salt or drought stress tolerance possibly through interacting with LIP5 in tobacco. This study may facilitate our understandings of the biological roles of SKD1-mediated ESCRT pathway under stress conditions in higher plants and accelerate genetic improvement of crop plants tolerant to environmental stresses.

  4. Rat and poultry feeding studies with soybean meal produced from imidazolinone-tolerant (CV127) soybeans.

    Science.gov (United States)

    He, Xiaoyun; de Brum, Paulo A R; Chukwudebe, Amechi; Privalle, Laura; Reed, Andrew; Wang, Yanqing; Zhou, Cui; Wang, Cuiyan; Lu, Jing; Huang, Kunlun; Contri, Daniela; Nakatani, Andreia; de Avila, Valdir S; Klein, Claudete H; de Lima, Gustavo J M M; Lipscomb, Elizabeth A

    2016-02-01

    The safety and nutritional properties of CV127 soybeans were evaluated in rat and broiler feeding studies. Some episodic differences were observed between rats fed CV127, Conquista, and the standard diet for the endpoints examined. None of these differences were considered treatment related, adverse, or biologically meaningful. In general, birds fed diets containing CV127, Conquista, or Monsoy 8001 showed no significant differences in growth and performance response variables. Chickens fed diets containing Coodetec 217 had lower body weight and weight gain for all developmental periods compared to CV127, but no significant differences were found in feed conversion for the two diets during any development period. The results of both feeding studies demonstrate that CV127 soybeans are as safe, wholesome, and nutritionally valuable as the other soybean meals tested, including those varieties for which histories of safe use have been established and well documented.

  5. Dosimetric accuracy and clinical quality of Acuros XB and AAA dose calculation algorithm for stereotactic and conventional lung volumetric modulated arc therapy plans

    International Nuclear Information System (INIS)

    The main aim of the current study was to assess the dosimetric accuracy and clinical quality of volumetric modulated arc therapy (VMAT) plans for stereotactic (stage I) and conventional (stage III) lung cancer treatments planned with Eclipse version 10.0 Anisotropic Analytical Algorithm (AAA) and Acuros XB (AXB) algorithm. The dosimetric impact of using AAA instead of AXB, and grid size 2.5 mm instead of 1.0 mm for VMAT treatment plans was evaluated. The clinical plan quality of AXB VMAT was assessed using 45 stage I and 73 stage III patients, and was compared with published results, planned with VMAT and hybrid-VMAT techniques. The dosimetric impact on near-minimum PTV dose (D98%) using AAA instead of AXB was large (underdose up to 12.3%) for stage I and very small (underdose up to 0.8%) for stage III lung treatments. There were no significant differences for dose volume histogram (DVH) values between grid sizes. The calculation time was significantly higher for AXB grid size 1.0 than 2.5 mm (p < 0.01). The clinical quality of the VMAT plans was at least comparable with clinical qualities given in literature of lung treatment plans with VMAT and hybrid-VMAT techniques. The average mean lung dose (MLD), lung V20Gy and V5Gy in this study were respectively 3.6 Gy, 4.1% and 15.7% for 45 stage I patients and 12.4 Gy, 19.3% and 46.6% for 73 stage III lung patients. The average contra-lateral lung dose V5Gy-cont was 35.6% for stage III patients. For stereotactic and conventional lung treatments, VMAT calculated with AXB grid size 2.5 mm resulted in accurate dose calculations. No hybrid technique was needed to obtain the dose constraints. AXB is recommended instead of AAA for avoiding serious overestimation of the minimum target doses compared to the actual delivered dose

  6. Rumen parameters of sheep fed Arachis pintoi cv. Belmonte hay

    Directory of Open Access Journals (Sweden)

    Gisele Machado Fernandes

    2012-12-01

    Full Text Available Studies on animal nutrition have shown factors that affect livestock yield and their interaction with the environment, through assessments on food nutritional values, nutrient metabolism in livestock and biochemical parameters related to nutrition and animal breeding. Inclusion levels of hay Arachis pintoi cv. Belmonte in the diet of sheep were studied by measuring the dry matter intake (DMI, production of short chain fatty acids (SCFA, ammonia nitrogen (NH3-N and rumen pH. Four males Santa Inês sheep with cannulas in the rumen were used in a 4x4 Latin Square design with four periods (21 days each and four treatments that corresponded to the inclusion levels (0%, 30%, 60% and 100% of Arachis pintoi cv. Belmonte to replace grass hay Cynodon dactylon cv. Coastcross in the diet. The DMI showed a quadratic effect increased until treatment with 60% of Arachis and reduced in the treatment with 100% of the legume. Significant interaction was observed between treatments and sampling times for NH3-N and acetate, propionate and butyrate concentration and the acetate:propionate ratio. There was no interaction between treatments and sampling time for rumen pH and total VFA concentration. It is observed a rapid increase in ammonia concentration until 2 hours after the feeding, and then a decline is seen. At 8 hours after the feeding, the quadratic effect occurred (P0.05 increasing NH3-N concentration with 60% of Arachis, declining afterwards. The acetate, propionate and butyrate concentration, showed a quadratic effect (P0.01 in the sampling time after the feeding for treatment with 60% of Arachis with the concentration rising until 4 hours and declining thereafter. For total SCFA concentration, when analyzed individually, we observed the effect of sampling time (P0.05, in which the means showed a quadratic effect, with maximum increase until 4 hours after the feeding, reducing at 8 hours. For acetate:propionate ratio was a linear effect (P0

  7. CV characteristics of polycrystalline sige films with low GE concentration

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, Ricardo Cotrin [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil); Doi, Ioshiaki [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil)]. E-mail: doi@led.unicamp.br; Diniz, Jose Alexandre [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil); Swart, Jacobus Willibrordus [School of Electrical and Computer Engineering (FEEC) and Center for Semiconductor Components(CCS), State University of Campinas - UNICAMP, Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil); Pinto Zakia, Maria Beny [Center for Semiconductor Components (CCS), State University of Campinas (UNICAMP), Cidade Universitaria Zeferino Vaz, Rua Pandia Calogeras, 90, Caixa Postal 6061, CEP: 13083-870, Campinas-SP (Brazil)

    2006-12-15

    SiGe alloys are currently used for HBT and MOS as epitaxial layers for base or strained channel, respectively. In the poly phase, SiGe has been studied as a replacement for poly-Si in MOS gates due to its lower thermal budget and gate depletion and also due to the Workfunction Engineering for V {sub t} adjustments. However, for application to CMOS technology as poly-SiGe gates, others constrains emerge such as quality of the oxide interface and etch chemistry. For both applications, the Ge fraction normally lies between 20% and 40%. In this study, authors use a low Ge contents (1%) poly-SiGe thin films aiming for MOS gate electrode. The Ge fraction was determined by RBS analysis. 230 nm thick samples were deposited onto 10 nm thermally oxidized <1 0 0>, p-type Si substrates using silane and germane. Films were deposited in the temperature of 500 deg. C and total pressure of 667 Pa (5 Torr) by vertical LPCVD. The samples were doped using {sup 31}P{sup +} ion implantation from 5 x 10{sup 14} cm{sup -2} up to 2 x 10{sup 16} cm{sup -2} and annealed by RTP (40 s) from 500 deg. C up to 900 deg. C. R {sub s} values were obtained by 4-point probe technique and CV curves were extracted from nMOS capacitors with 200 {mu}m diameter. The same processing steps were used to fabricate similar poly-Si samples and capacitors for comparison. The poly-SiGe samples presented R {sub s} values one order of magnitude lower than poly-Si and CV analysis of nMOS capacitors showed very good characteristics. The 1% Ge in the alloy ensures a low thermal budget for the overall process. Although a relatively high annealing temperature (800 deg. C) must be used to reduce oxide charge and interface traps, the temperature is well below the necessary for poly-Si processing and can allow formation of the shallow junctions needed for next technological nodes.

  8. STRATEGI PENGEMBANGAN USAHA SAPI POTONG (STUDI KASUS CV MITRA TANI FARM

    Directory of Open Access Journals (Sweden)

    Shally Alpriany Aisyah

    2014-09-01

    Full Text Available ABSTRACTThe purpose of this study were to 1 analyze the internal and external environmental conditions of CV Mitra Tani Farm, 2 identify and define strategies that affect the cattle breeding business development at CV Mitra Tani Farm, and 3 set the strategic priorities in the development of cattle breeding business in CV Mitra Tani Farm. Analysis strategies model (David, 2009 used were the analysis of the internal and external environment, strategy formulation and strategic priorities. At the stage of strategy formulation, SWOT 4 Quadrants were used. The results showed that the difference between the weighted scores of opportunities and threats as well as strengths and weaknesses is located in first quadrant of the SWOT 4 quadrant matrix. The first quadrant (growth quadrant has eight strategies, but only three can be applied appropriately. The results of the value of interest using quantitative strategic planning matrix strategy used is the market expansion strategy, then the strategy of product development, and concentric diversification strategy. Therefore, in carrying out market expansion strategy, CV Mitra Tani Farm needs to do breakthrough campaign. In product development strategy, preferably CV Mitra Tani Farm makes processed beef products which are unique and still rare in the market today.Keywords: strategy, business beef cattle, CV Mitra Tani Farm, SWOT 4K, QSPMABSTRAKTujuan penelitian ini yaitu 1 menganalisis kondisi lingkungan internal dan eksternal CV Mitra Tani Farm, 2 mengidentifikasi dan menetapkan strategi yang memengaruhi dalam pengembangan bisnis sapi potong di CV Mitra Tani Farm, dan 3 menetapkan prioritas strategi dalam pengembangan usaha peternakan sapi potong di CV Mitra Tani Farm. Model analisis strategi (David, 2009 yang digunakan yaitu analisis lingkungan internal dan eksternal, perumusan strategi dan prioritas strategi. Pada tahap perumusan strategi digunakan SWOT 4 Kuadran. Hasil penelitian menunjukkan bahwa selisih

  9. Infrared small target detection technology based on OpenCV

    Science.gov (United States)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  10. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean

    Institute of Scientific and Technical Information of China (English)

    Yau Sang Chan; Jack Ho Wong; Evandro Fei Fang; Wen Liang Pan; Tzi Bun Ng

    2012-01-01

    A 5.4-kDa antifungal peptide,with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC5o=3 μM),Setospaeria turcica and Bipolaris maydis,was isolated from the seeds of Phaseolus vulgaris cv.brown kidney bean.The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75.The antifungal activity of the peptide against M.arachidicola was stable in the pH range 3-12 and in the temperature range 0℃ to 80℃.There was a slight reduction of the antifungal activity at pH 2 and 13,and the activity was indiscernible at pH 0,1,and 14.The activity at 90℃ and 100℃ was slightly diminished.Deposition of Congo red at the hyphal tips of M.arachidicola was induced by the peptide indicating inhibition of hyphal growth.The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells,in contrast to the presence of such activity of other antifungal peptides,indicates that different domains are responsible for the antifungal and antiproliferative activities.

  11. Nitrogen fertilization on the establishment of Arachis pintoi cv. Belmonte

    Directory of Open Access Journals (Sweden)

    Rita Manuele Porto Sales

    2012-11-01

    Full Text Available The objective was to evaluate the effect of nitrogen fertilization on the establishment of forage peanut (Arachis pintoi cv. Belmonte propagated vegetatively. The experiment was conducted in a greenhouse in a completely randomized design with treatments arranged in a 2 × 4 factorial design - two ages (70 and 85 days after planting and four nitrogen doses (0, 40, 80 and 120 kg/ha - with four replications. Morphogenetic and structural characteristics and production were evaluated. The nitrogen accelerated the establishment of the forage peanut with an increase in dry weight of green leaves and stolons. The greatest length of stolons (48.0 cm was obtained with a dose equivalent to 86 kg N/ha and higher density of stolons (20 stolons/vase between 78 and 82 kg N/ha. Nitrogen fertilization also reduced the phyllochron from 6.7 to 4.6 days/leaf. These data were more intense at 85 days, suggesting greater photosynthetic contribution during this period related to the large number of leaves after 70 days. Therefore, nitrogen can be an important tool to accelerate the establishment of pure stands of forage peanut.

  12. A virtual computing infrastructure for TS-CV SCADA systems

    CERN Document Server

    Poulsen, S

    2008-01-01

    In modern data centres, it is an emerging trend to operate and manage computers as software components or logical resources and not as physical machines. This technique is known as â€ワvirtualisation” and the new computers are referred to as â€ワvirtual machines” (VMs). Multiple VMs can be consolidated on a single hardware platform and managed in ways that are not possible with physical machines. However, this is not yet widely practiced for control system deployment. In TS-CV, a collection of VMs or a â€ワvirtual infrastructure” is installed since 2005 for SCADA systems, PLC program development, and alarm transmission. This makes it possible to consolidate distributed, heterogeneous operating systems and applications on a limited number of standardised high-performance servers in the Central Control Room (CCR). More generally, virtualisation assists in offering continuous computing services for controls and maintaining performance and assuring quality. Implementing our systems in a vi...

  13. The early-type close binary CV Velorum revisited

    CERN Document Server

    Yakut, K; Morel, T; Morel, Th.

    2007-01-01

    Our goal was to improve the fundamental parameters of the massive close double-lined eclipsing B2.5V+B2.5V binary CV Velorum.We gathered new high-resolution echelle spectroscopy on 13 almost consecutive nights covering essentially two orbits. We computed a simultaneous solution to all the available high-quality radial-velocity and light data with the latest version of the Wilson-Deviney code. We obtained the following values for the physical parameters: $M_1 = 6.066(74) M_\\odot$, $M_2 = 5.972(70) M_\\odot$, $R_1 = 4.126(24) R_\\odot$, $R_2 = 3.908(27) R_\\odot$, $\\log L_1 = 3.20(5) L_\\odot$, and $\\log L_2 = 3.14(5) L_\\odot$. The quoted errors contain a realistic estimate of systematic uncertainties mainly stemming from the effective temperature estimation. We derived abundances for both components and found them to be compatible with those of B stars in the solar neighbourhood. We discovered low-amplitude periodic line-profile variations with the orbital frequency for both components. Their interpretation requir...

  14. Seven health physics calculator programs for the HP-41CV

    International Nuclear Information System (INIS)

    Several user-oriented programs for the Hewlett-Packard HP-41CV are explained. The first program builds, stores, alters, and ages a list of radionuclides. This program only handles single- and double-decay chains. The second program performs convenient conversions for the six nuclides of concern in plutonium handling. The conversions are between mass, activity, and weight percents of the isotopes. The source can be aged and/or neutron generation rates can be computed. The third program is a timekeeping program that improves the process of manually estimating and tracking personnel exposure during high dose rate tasks by replacing the pencil, paper, and stopwatch method. This program requires a time module. The remaining four programs deal with computations of time-integrated air concentrations at various distances from an airborne release. Building wake effects, source depletion by ground deposition, and sector averaging can all be included in the final printout of the X/Q - Hanford and X/Q - Pasquill programs. The shorter versions of these, H/Q and P/Q, compute centerline or sector-averaged values and include a subroutine to facilitate dose estimation by entering dose factors and quantities released. The horizontal and vertical dispersion parameters in the Pasquill-Gifford programs were modeled with simple, two-parameter functions that agreed very well with the usual textbook graphs. 8 references, 7 appendices

  15. MICROPROPAGAÇÃO DO KIWI CV. HAYWARD

    Directory of Open Access Journals (Sweden)

    SCALIZE FÁBIO EDUARDO

    2001-01-01

    Full Text Available O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch, cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962 -- MS, suplementado com dois tipos de auxina (AIA e AIB e uma citocinina (BAP. Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1, combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1 na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1, independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.

  16. Comportamento de Andropogon gayanus cv. ‘planaltina’ e Panicum maximum cv. ‘tanzânia’ sob sombreamento Performance of Andropogon gayanus and Panicum maximum cv. ‘Tanzania’ in the shading

    OpenAIRE

    Fabiana Lopes Ramos de Oliveira; Verônica Alves Mota; Maira Soares Ramos; Leonardo David Tuffi Santos; Neide Judith Faria de Oliveira; Luciana Castro Geraseev

    2013-01-01

    A utilização de forrageiras tolerantes ao sombreamento é fundamental para a sustentabilidade da integração lavoura-pecuária-floresta. Estudou-se o comportamento do Panicum maximum cv. 'Tanzânia' e do Andropogon gayanus cv. 'Planaltina' sob diferentes níveis de sombreamentos e épocas de entrada sob a sombra. Utilizou-se delineamento em blocos casualizados, com quatro repetições, em esquema fatorial com dois tipos de sombreamento: 30% e 50%; três épocas de entrada na sombra após transplante (07...

  17. The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice.

    Science.gov (United States)

    Zhu, Xiaobo; Yin, Junjie; Liang, Sihui; Liang, Ruihong; Zhou, Xiaogang; Chen, Zhixiong; Zhao, Wen; Wang, Jing; Li, Weitao; He, Min; Yuan, Can; Miyamoto, Koji; Ma, Bingtian; Wang, Jichun; Qin, Peng; Chen, Weilan; Wang, Yuping; Wang, Wenming; Wu, Xianjun; Yamane, Hisakazu; Zhu, Lihuang; Li, Shigui; Chen, Xuewei

    2016-09-01

    Previous studies have shown that multivesicular bodies (MVBs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. However, the molecular regulation is poorly understood in rice. Here we report the identification and characterization of a MVBs-localized AAA ATPase LRD6-6 in rice. Disruption of LRD6-6 leads to enhanced immunity and cell death in rice. The ATPase activity and homo-dimerization of LRD6-6 is essential for its regulation on plant immunity and cell death. An ATPase inactive mutation (LRD6-6E315Q) leads to dominant-negative inhibition in plants. The LRD6-6 protein co-localizes with the MVBs marker protein RabF1/ARA6 and interacts with ESCRT-III components OsSNF7 and OsVPS2. Further analysis reveals that LRD6-6 is required for MVBs-mediated vesicular trafficking and inhibits the biosynthesis of antimicrobial compounds. Collectively, our study shows that the AAA ATPase LRD6-6 inhibits plant immunity and cell death most likely through modulating MVBs-mediated vesicular trafficking in rice. PMID:27618555

  18. Comparison of build-up region doses in oblique tangential 6 MV photon beams calculated by AAA and CCC algorithms in breast Rando phantom

    Science.gov (United States)

    Masunun, P.; Tangboonduangjit, P.; Dumrongkijudom, N.

    2016-03-01

    The purpose of this study is to compare the build-up region doses on breast Rando phantom surface with the bolus covered, the doses in breast Rando phantom and also the doses in a lung that is the heterogeneous region by two algorithms. The AAA in Eclipse TPS and the collapsed cone convolution algorithm in Pinnacle treatment planning system were used to plan in tangential field technique with 6 MV photon beam at 200 cGy total doses in Breast Rando phantom with bolus covered (5 mm and 10 mm). TLDs were calibrated with Cobalt-60 and used to measure the doses in irradiation process. The results in treatment planning show that the doses in build-up region and the doses in breast phantom were closely matched in both algorithms which are less than 2% differences. However, overestimate of doses in a lung (L2) were found in AAA with 13.78% and 6.06% differences at 5 mm and 10 mm bolus thickness, respectively when compared with CCC algorithm. The TLD measurements show the underestimate in buildup region and in breast phantom but the doses in a lung (L2) were overestimated when compared with the doses in the two plannings at both thicknesses of the bolus.

  19. AAA安全管理系统在数字图书馆中的应用%Application of AAA Secure Management Information System to Digital Library

    Institute of Scientific and Technical Information of China (English)

    雷向康

    2009-01-01

    针对当前数字图书馆网络系统设备中普遍存在的安全隐患问题,提出在各网络设备中使用基于AAA(认证、授权、记账)的方法,在管理和控制端,使用一套Cisco Secure ACS(思科安全访问控制服务器)软件, 用TACACS+或RADIUS协议与ACS进行通信认证.AAA安全管理系统部署后,成功地解决了这一难题,实验证明本方法行之有效.%In order to overcome the common obstacle of secure and peril problems of the switchs in digital library networks, this paper develops the method based on AAA(Authentication、Authorization、Accounting) in client switchs of networks and Cisco Secure ACS(Access Control Server)in management server,by communication between switchs and ACS through protocol of TACACS+ or RADIUS.The method is tested successfully and proved effectively in our ditital library.

  20. Multicenter Orthopaedic Outcome Network Early Anti-inflammatory Treatment in Patients with Acute ACL Tear” (MOON-AAA) Clinical Trial

    Science.gov (United States)

    Lattermann, Christian; Proffitt, Mary; Huston, Laura J.; Gammon, Lee; Johnson, Darren L.; Kraus, Virginia B.; Spindler, Kurt P.

    2016-01-01

    Objectives: We present the early results from the “Multicenter Orthopaedic Outcome Network Early Anti-inflammatory Treatment in Patients with Acute ACL Tear and Painful Effusions” (MOON-AAA) clinical trial (figure 1). This trial allows for a well controlled prospective cohort of patients with isolated ACL injury at risk for OA. We compared the effect of a single versus a repeated dosage of Kenalog within the first two weeks after ACL injury and its effect on chondral degradation in the first 4 weeks prior to surgical reconstruction of the ACL. Methods: 49 patients with isolated ACL tears were enrolled. Knee joints were aspirated and patients received an injection with 40 mg Kenalog either within 4 days, 10 days, both time points or not at all (saline injection control). Serum, synovial fluid and urine were collected at 3 time points. Permutated block randomization, triple blinding, independent monitoring and standardized x-ray was performed to comply with GCP standards. Patient reported outcomes were collected at 6 time points up to 6 months post-ACL reconstruction(IKDC, KOOS and Marx activity level). A standardized synovial fluid biomarker panel was analyzed according to OARSI guidelines. Statistical analysis were performed using SAS mixed models analysis. Results: Serum analysis shows significant change after injury. Chondrodegradatory markers such as CTX-II, MMP-1 and MMP-3 as well as COMP indicate a progressive destruction of chondral matrix and collagen breakdown . There is a dramatic (250%) increase of CTX-II in the first 4 weeks. Matrix proteins such as MMP-1 and 3 as well as COMP show an initial increase and then a steep decline (see figure 1). Inflammatory markers (IL-1 alpha, IL-1beta, IRAP) show a decline from the time of injury. IL-1 alpha, however shows a dramatic uptake after week 2. This longitudinal data confirms a dramatic onset of early osteoarthritic biomarker profiles immediately after ACL injury as measured in synovial fluid

  1. Influence of Lachancea thermotolerans on cv. Emir wine fermentation.

    Science.gov (United States)

    Balikci, Eren Kemal; Tanguler, Hasan; Jolly, Neil P; Erten, Huseyin

    2016-07-01

    The present paper describes the behaviour of Lachancea thermotolerans and Saccharomyces cerevisiae in pure, co-cultured and sequential fermentations in cv. Emir grape must. Faster fermentation rates were observed in wine made with a pure culture of S. cerevisiae and wine produced with simultaneously inoculated cultures of L. thermotolerans and S. cerevisiae. Both L. thermotolerans and S. cerevisiae gave high population numbers. The use of L. thermotolerans in mixed and sequential cultures led to an increase in final total acidity content in the wines, varying in the range 5.40-6.28 g/l (as tartaric acid), compared to pure culture S. cerevisiae, which gave the lowest level of total acidity (5 g/l). The increase was in the order of 1.18-2.06 g/l total acidity. Increase in final acidity by the use of L. thermotolerans might be useful to improve wines with low acidity due to global climate change. Volatile acidity levels (as acetic acid) were in the range 0.53-0.73 g/l, while the concentration of ethyl alcohol varied in the range 10.76-11.62% v/v. Sequential fermentations of wines and pure culture fermentation of L. thermotolerans resulted in reduction in the concentrations of acetaldehyde and higher alcohols, with exception of N-propanol and esters. According to the sensory analysis, wine obtained with sequential inoculation of L. thermotolerans followed by inoculation of S. cerevisiae after 24 h, and simultaneous inoculation of these yeasts, was the most preferred. Copyright © 2016 John Wiley & Sons, Ltd. PMID:27113383

  2. High Frequency Plant Regeneration of Musa paradisiaca cv. Karibale Monthan

    Directory of Open Access Journals (Sweden)

    R. Shashi Kumar

    2015-06-01

    Full Text Available High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 % was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l, TDZ (0.1 to 1.2 mg/l and coconut water (0.1 to 0.9 ml/l induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg, number of hands in a bunch, number of fingers in a hand, length of the finger (cm, girth of the finger (cm and girth of the pseudostem (cm exhibited by in vitro plants were higher than the in vivo plants.

  3. OBSERVATION ON THE THERAPEUTIC EFFECT OF ACU-MOXIBUSTION OF GUANYUAN (CV 4) AND QIHAI (CV 6) IN THE TREATMENT OF 56 CASES OF HICCUP

    Institute of Scientific and Technical Information of China (English)

    LI Jun-qing

    2005-01-01

    @@ Hiccup is a symptom characterized by a short, repeated involuntary sound from the larynx due to reversed upward flow of qi to stimulate the diaphragm (spasm of the diaphragm), followed by sudden closure of the glottis, which checks the inflow of air. It may occur along or appear in the developmental process of some acute or chronic diseases, and is often a sign of deterioration of the disease. From 2000 to 2004, the author of the present paper treated 56 cases of hiccup with acu-moxibustion of Guanyuan (关元CV 4) and Qihai (气海CV 6) and achieved satisfied results. Following is the summary.

  4. Caracterização do grânulo de amido de bananas (Musa AAA-Nanicão e Musa AAB-Terra Characterization of starch granules from bananas Musa AAA-Nanicão and Musa AAB-Terra

    Directory of Open Access Journals (Sweden)

    M.C.J. Freitas

    2005-06-01

    Full Text Available O amido de bananas tem sido pesquisado na área de nutrição a partir da introdução do conceito de Amido Resistente. O amido de Musa AAA-Nanicão e Musa AAB-Terra foram caracterizados quanto as suas respostas fisiológicas [12]. Em continuidade, o presente trabalho estudou características físicas e morfológicas dos grânulos de amido de ambas as espécies de banana comparando-as com amido nativo de milho comercial. O amido de bananas foi extraído segundo CHIANG, CHU & CHU [3]. A morfologia dos grânulos foi realizada após tratamento enzimático in vitro a 37°C/24h com alfa-amilase pancreática. Foram efetuados os respectivos amilogramas e difractogramas de raios-X. Os grânulos de amido da Musa Tipo AAA-Nanicão apresentaram comprimento entre 30-40µm. Em Musa AAB-Terra, os grânulos, também ovais e alongados, eram um pouco menores, 20-30µm. A corrosão enzimática in vitro iniciava-se sobre a superfície anteriormente lisa e formavam estrias superficiais e apicais. A Microscopia Eletrônica de Varredura (MEV mostrou que a hidrólise in vitro por 24 horas foi pequena e ocorria sobretudo nas camadas amorfas dos grânulos de ambas as espécies. O padrão de corrosão demonstrou-se distinto daquele ocorrido no amido de milho. As suspensões de amido de bananas ao viscosímetro demonstraram forte capacidade de hidratação e menor capacidade de retrogradação em relação ao milho, sobretudo do amido de Musa Tipo AAA-Nanicão; o amido de Musa Tipo AAB-Terra apresentou maior estabilidade de pasta. Na análise de difração de raios-X, os grânulos de bananas apresentaram padrão tipo B e C para Musa Tipo AAA-Nanicão e Musa Tipo AAB-Terra, respectivamente. Conclui-se que os amidos de Musa AAA-Nanicão e Musa AAB-Terra são estruturalmente distintos, justificando as respostas fisiológicas distintas encontradas posteriormente pelos mesmos autores. As distinções das propriedades físicas e bioquímicas obtidas para os grânulos, embora

  5. CV Keskuse müünud äriduo võtab sihikule Rumeenia

    Index Scriptorium Estoniae

    2007-01-01

    CV Keskuse omanikud Raivo Heina ja Erko Kundla müüsid kaheksa aastat pärast ettevõtte loomist sellest 75% Vene, USA ja Inglismaa investoritele. Osa tehingust saadud rahast on plaanis investeerida Rumeeniasse

  6. Breeding of Chamaecrista rotundifolia CV. Minyu No.1 by 60Cy γ-rays irradiation

    International Nuclear Information System (INIS)

    Chamaecrista rotundifoli CV. Minyu No.1 was selected and bred by Agricultural Ecology Institute of Fujian Academy of Agricultural Sciences, which was an improved mutant strain from Chamaecrista rotundifolia cv. Minyin through 60Cs γ-rays irradiation in 2002. It was passed the evaluation of National Grass Variety Approval Committee in 2011. Variety comparison trial, regional test and productive trial were carried out during 2005 to 2010. The results showed that the hay yield was up to 10000 ∼ 15000 kg/hm2, which was 20.70% higher than that of C. rotundifolia cv. Minyin (the control group) (P2, which was increased by 9.01% compared with the control group (P>0.05). The crude protein content was 19.15% when harvested at initial blooming stage. In summary, C. rotundifoli CV. Minyu No.1 was suitable to plant in tropical and sub-tropical red soil regions in Southern China. (authors)

  7. Analisis Strategi Bisnis Melalui Marketing Mix terhadap Penjualan pada CV. Citra Anugrah

    OpenAIRE

    Rabino, Rendi

    2015-01-01

    CV. Citra Anugrah Medan is a company that engage in buying and selling second car which still appertain as a new company. Founded on January 21, 2012, the company is still struggling in trying to increase the selling point. The research intends to determine the relationship between marketing mix strategy in increasing CV. Citra Anugrah selling point by discovering the marketing mix strategies which the company utilize. This research includes the utilization of descriptive analysis method w...

  8. Plug-in de procesado visual (OpenCV) en OpenDomo OS

    OpenAIRE

    Herrera Castro, Alexander

    2015-01-01

    Este proyecto consiste en el diseño y desarrollo de un plug-in que permita usar el sistema de procesado de imagen OpenCV desde el sistema operativo OpenDomo OS. Aquest projecte consisteix en el disseny i desenvolupament d'un plug-in que permeti utilitzar el sistema de processament d'imatge OpenCV des del sistema operatiu OpenDomo OS. Master thesis for the Free Software program.

  9. Working with OpenCV and Intel Image Proccessing Libraries. Proccessing image data tools

    OpenAIRE

    Mora Lizán, Francisco José; Llorens Largo, Faraón; Pujol López, Mar; Rizo Aldeguer, Ramón; Villagrá Arnedo, Carlos

    2002-01-01

    We will provide an overview of Intel OpenCV and Image Processing Libraries. We present an application of real-time gesture recognition using the libraries (segmenting a foreground object, creating Motion History Image (MHI), updating the intensity gradients, and recovering directional motion information). Some times most companies spent a lot of time and money implementing those well-known techniques. OpenCV and IPL implement a huge amount of standard and advanced image processing techniqu...

  10. Biofuels from the Fresh Water Microalgae Chlorella vulgaris (FWM-CV for Diesel Engines

    Directory of Open Access Journals (Sweden)

    Saddam H. Al-lwayzy

    2014-03-01

    Full Text Available This work aims to investigate biofuels for diesel engines produced on a lab-scale from the fresh water microalgae Chlorella vulgaris (FWM-CV. The impact of growing conditions on the properties of biodiesel produced from FWM-CV was evaluated. The properties of FWM-CV biodiesel were found to be within the ASTM standards for biodiesel. Due to the limited amount of biodiesel produced on the lab-scale, the biomass of dry cells of FWM-CV was used to yield emulsified water fuel. The preparation of emulsion fuel with and without FWM-CV cells was conducted using ultrasound to overcome the problems of large size microalgae colonies and to form homogenized emulsions. The emulsified water fuels, prepared using ultrasound, were found to be stable and the size of FWM-CV colonies were effectively reduced to pass through the engine nozzle safely. Engine tests at 3670 rpm were conducted using three fuels: cottonseed biodiesel CS-B100, emulsified cottonseed biodiesel water fuel, water and emulsifier (CS-E20 and emulsified water containing FWM-CV cells CS-ME20. The results showed that the brake specific fuel consumption (BSFC was increased by about 41% when the engine was fueled with emulsified water fuels compared to CS-B100. The engine power, exhaust gas temperature, NOx and CO2 were significantly lower than that produced by CS-B100. The CS-ME20 produced higher power than CS-E20 due to the heating value improvement as a result of adding FWM-CV cells to the fuel.

  11. Kebiasaan Makanan Ikan Baung (Mystusnemurus C.V) di Sungai Bingai Binjai Provinsi Sumatera Utara.

    OpenAIRE

    Windy

    2015-01-01

    Research of Baung’s food (Mystus nemurus CV) was performed in Bingai river in June to August 2014. The purpose of this study was to determine the type of natural food and the availability of natural forage baung (Mystus nemurus CV) in the Bingai river and general condition of the waters of the Bingai. Stomach contents were analyzed by using Index of preponderance. Stomach contents consisted of 8 types of fish, plant fibers, Thiara scabra, Planaria sp., Nodilittorina pyramidalis, Faunus ater, ...

  12. Physicochemical Composition, Phenolic Content and Antioxidant Activity of Sour Cherry cv. Marasca During Ripening

    OpenAIRE

    Sandra Pedisić; Branka Levaj; Verica Dragović-uzelac; Kristina Kos

    2007-01-01

    Sour cherry cv. Marasca is Dalmatian cultivar from XVI century. Cultivation is limited on the north and central part of Dalmatia and on the part of the islands, where it achieves the best quality of fruit, high content of dry matter and sugar respectively, agreeable aroma and intense color. Sour cherry cv. Marasca is source of biologically active ingredients, organic and inorganic compounds, dietary fibers, aromatic compounds and high content of phenolic compounds, particularly anthocyanins a...

  13. Geometrical determinations of IMRT photon pencil-beam path in radiotherapy wedges and limit divergence angle with the Anisotropic Analytic Algorithm (AAA

    Directory of Open Access Journals (Sweden)

    Francisco Casesnoves

    2014-08-01

    Full Text Available Purpose: Static wedge filters (WF are commonly used in radiation therapy, forward and/or inverse planning. We calculated the exact 2D/3D geometrical pathway of the photon-beam through the usual alloy WF, in order to get a better dose related to the beam intensity attenuation factor(s, after the beam has passed through the WF. The objective was to provide general formulation into the Anisotropic Analytical Algorithm (AAA model coordinates system (depending on collimator/wedge angles that also can be applied to other models. Additionally, second purpose of this study was to develop integral formulation for 3D wedge exponential factor with statistical approximations, with introduction for the limit angle/conformal wedge.Methods: The radiotherapy model used to develop this mathematical task is the classical superposition-convolution algorithm, AAA (developed by Ulmer and Harder. We worked with optimal geometrical approximations to make the computational IMRT calculations quicker/reduce the planning-system time. Analytic geometry/computational-techniques to carry out simulations (for standard wedges are detailed/developed sharply. Integral developments/integral-statistical approximations are explained. Beam-divergence limit Angle for optimal wedge filtration formulas is calculated/sketched, with geometrical approximations. Fundamental trigonometry is used for this purpose.Results: Extent simulation tables for WF of 15º, 30º, 45º, and 60º are shown with errors. As a result, it is possible to determine the best individual treatment dose distribution for each patient. We presented these basic simulations/numerical examples for standard manufacturing WF of straight sloping surface, to check the accuracy/errors of the calculations. Simulations results give low RMS/Relative Error values (formulated for WF of 15º, 30º, 45º, and 60º.Conclusion: We obtained a series of formulas of analytic geometry for WF that can be applied for any particular dose

  14. The evaluation of the dose calculation algorithm(AAA)'s accuracy in case of a radiation therapy on inhomogeneous tissues using FFF beam

    Energy Technology Data Exchange (ETDEWEB)

    Kim, In Woo; Chae, Seung Hoon; Kim, Min Jung; Kim, Bo Gyoum; Kim, Chan Yong; Park, So Yeon; Yoo, Suk Hyun [Dept. of Radiation Oncology, Seoul National University Hospital, Seoul (Korea, Republic of)

    2014-12-15

    To verify the accuracy of the Ecilpse's dose calculation algorithm(AAA:Analytic anisotropic algorithm) in case of a radiation treatment on Inhomogeneous tissues using FFF beam comparing dose distribution at TPS with actual distribution. After acquiring CT images for radiation treatment by the location of tumors and sizes using the solid water phantoms, cork and chest tumor phantom made of paraffin, we established the treatment plan for 6MV photon therapy using our radiation treatment planning system for chest SABR, Ecilpse's AAA(Analytic anisotropic algorithm). According to the completed plan, using our TrueBeam STx(Varian medical system, Palo Alto, CA), we irradiated radiation on the chest tumor phantom on which EBT2 films are inserted and evaluated the dose value of the treatment plan and that of the actual phantom on Inhomogeneous tissue. The difference of the dose value between TPS and measurement at the medial target is 1.28-2.7%, and, at the side of target including inhomogeneous tissues, the difference is 2.02%-7.40% at Ant, 4.46%-14.84% at Post, 0.98%-7.12% at Rt, 1.36%-4.08% at Lt, 2.38%-4.98% at Sup, and 0.94%-3.54% at Inf. In this study, we discovered the possibility of dose calculation's errors caused by FFF beam's characteristics and the inhomogeneous tissues when we do SBRT for inhomogeneous tissues. SBRT which is most popular therapy method needs high accuracy because it irradiates high dose radiation in small fraction. So, it is supposed that ideal treatment is possible if we minimize the errors when planning for treatment through more study about organ's characteristics like Inhomogeneous tissues and FFF beam's characteristics.

  15. TU-C-BRE-05: Clinical Implications of AAA Commissioning Errors and Ability of Common Commissioning ' Credentialing Procedures to Detect Them

    Energy Technology Data Exchange (ETDEWEB)

    McVicker, A; Oldham, M; Yin, F; Adamson, J [Duke University Medical Center, Durham, NC (United States)

    2014-06-15

    Purpose: To test the ability of the TG-119 commissioning process and RPC credentialing to detect errors in the commissioning process for a commercial Treatment Planning System (TPS). Methods: We introduced commissioning errors into the commissioning process for the Anisotropic Analytical Algorithm (AAA) within the Eclipse TPS. We included errors in Dosimetric Leaf Gap (DLG), electron contamination, flattening filter material, and beam profile measurement with an inappropriately large farmer chamber (simulated using sliding window smoothing of profiles). We then evaluated the clinical impact of these errors on clinical intensity modulated radiation therapy (IMRT) plans (head and neck, low and intermediate risk prostate, mesothelioma, and scalp) by looking at PTV D99, and mean and max OAR dose. Finally, for errors with substantial clinical impact we determined sensitivity of the RPC IMRT film analysis at the midpoint between PTV and OAR using a 4mm distance to agreement metric, and of a 7% TLD dose comparison. We also determined sensitivity of the 3 dose planes of the TG-119 C-shape IMRT phantom using gamma criteria of 3% 3mm. Results: The largest clinical impact came from large changes in the DLG with a change of 1mm resulting in up to a 5% change in the primary PTV D99. This resulted in a discrepancy in the RPC TLDs in the PTVs and OARs of 7.1% and 13.6% respectively, which would have resulted in detection. While use of incorrect flattening filter caused only subtle errors (<1%) in clinical plans, the effect was most pronounced for the RPC TLDs in the OARs (>6%). Conclusion: The AAA commissioning process within the Eclipse TPS is surprisingly robust to user error. When errors do occur, the RPC and TG-119 commissioning credentialing criteria are effective at detecting them; however OAR TLDs are the most sensitive despite the RPC currently excluding them from analysis.

  16. FORMULASI STRATEGI PENINGKATAN PRODUKSI DOMBA CV MITRA TANI FARM, CIAMPEA, BOGOR

    Directory of Open Access Journals (Sweden)

    Amalia Sholehana

    2014-04-01

    Full Text Available ABSTRACTThis research was conducted to formulate to increase the sheep production at CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. The research was conducted using descriptive analysis by analyzing the problem experienced by CV. MitraTani Farm. Primary data and secondary data were gathered at this research. The research was also conducted using several analytical methods such as value chain mapping, internal factor evaluation (IFE and external factor evaluation (EFE matrix, SWOT and quantitative strategic planning matrix (QSPM. Based on the value chain analysis, the current production of sheep at CV MT farm is highly depending on the supply from its partners and internal farmers. According to the IFE and EFE results, the differences of each weighted values were respectively 2,120 and 0,686 so the position of the company in the SWOT matrix was situated at Quadrant I. Therefore the company needs to use a growth strategy. The QSPM sequenced the strategy priority as follow (1 increasing the number of lambs, (2 developing the management, (3 strengthening the capital and ownership, (4 improving the quality through technology. The result of the gross profit margin calculation, if the sheep‘s selling is increased up to 1000 sheep per month then the possible annual profit that can be earned by CV MT farm is 40,34% of the total production.Keywords: MT Farm, sheep production, strategies, SWOT, QSPM, gross profit marginABSTRAKPenelitian ini dilakukan untuk memformulasikan strategi peningkatan produksi domba di CV Mitra Tani Farm (CV MT Farm, Ciampea, Bogor. Pendekatan penelitian yang diterapkan adalah analisis deskriptif yang dilakukan dengan mempelajari permasalahan dari objek yang diteliti. Data yang dikumpulkan dalam penelitian ini adalah data primer dan data sekunder. Penelitian dilakukan menggunakan alat analisis berupa pemetaan rantai nilai,  matriks IFE (Internal Factor Evaluation dan EFE (External Factor Evaluation, SWOT, dan QSPM (Quantitative

  17. Conservação de tangerina cv. Clemenules utilizando diferentes recobrimentos = Conservation of Mandarins cv. Clemenules with different cuticles

    Directory of Open Access Journals (Sweden)

    Rosa de Oliveira Treptow

    2007-01-01

    Full Text Available O objetivo deste trabalho foi verificar os efeitos de diferentes recobrimentos e períodos de armazenamento na conservação de tangerinas Clemenules. Após a colheita, as frutas foram submetidas ao pré-resfriamento por 12 horas a 5ºC e em seguida, aos seguintes tratamentos: T1 testemunha; T2 filme de polietileno microperfurado; T3 filme de polietileno não perfurado; T4 cera de carnaúba a 50%, diluída em água; T5 cera de carnaúba a 100% (não diluída. As tangerinas foram armazenadas por 20, 40 e 60 dias em temperatura de 5ºC e UR de 90-95%, depois foram mantidas em temperatura de 15±1ºC e UR de 75-80%, durante quatro dias simulando a comercialização. Na colheita e após cada período de armazenamento, seguido de simulação decomercialização, foram avaliadas as variáveis: perda de massa; cor; sólidos solúveis (SS; acidez titulável (AT; relação SS/AT; podridões, distúrbios fisiológicos e características sensoriais. Amodificação da atmosfera reduziu a perda de massa, a incidência de distúrbios e podridões de tangerinas Clemenules durante o armazenamento. As frutas acondicionadas em filme de polietilenosem perfuração ou aquelas revestidas com cera sem diluição apresentaram sabor estranho e menor qualidade geral. Tangerinas ‘Clemenules’ acondicionadas em filme de polietileno microperfurado ecera diluída em 50% de água podem ser armazenadas durante 60 dias a 5ºC e comercializadas durante quatro dias a 15±1ºC sem comprometer a qualidade sensorial.The aim of this work was to evaluate the effects of different cuticles and storage periods on the conservation of mandarins cv. Clemenules. After harvest the fruits were kept at 5ºC during 12 hours. Then, they were submitted to the following treatments: T1 control; T2 micro perforated polyethylene bag; T3 non-perforated polyethylene bag; T4 carnauba wax at 50% in water; T5 carnauba wax at 100% (without dilution. The mandarins were stored for 20, 40 and 60 days at 5

  18. CV-990 Landing Systems Research Aircraft (LSRA) during final Space Shuttle tire test

    Science.gov (United States)

    1995-01-01

    A Convair 990 (CV-990) was used as a Landing Systems Research Aircraft (LSRA) at NASA's Dryden Flight Research Center, Edwards, California, to test space shuttle landing gear and braking systems as part of NASA's effort to upgrade and improve space shuttle capabilities. The first flight at Dryden of the CV-990 with shuttle test components occurred in April 1993, and tests continued into August 1995, when this photo shows a test of the shuttle tires. The purpose of this series of tests was to determine the performance parameters and failure limits of the tires. This particular landing was on the dry lakebed at Edwards, but other tests occurred on the main runway there. The CV-990, built in 1962 by the Convair Division of General Dynamics Corp., Ft. Worth, Texas, served as a research aircraft at Ames Research Center, Moffett Field, California, before it came to Dryden.

  19. Analysis of Positive Superhump Shapes Near Superoutburst Maximum in CV SU UMa-like Systems

    Science.gov (United States)

    Bobertz, Michele; Voloshina, Irina; Goel, Amit

    2016-01-01

    Positive superhumps are hump-shaped modulations in light curves of some Cataclysmic Variables (CVs) that have a period that is slightly longer than the orbital period. In CV SU UMa-like systems, the shape of the positive superhump is known to change throughout the superoutburst, which thus slightly changes the published, observed, positive superhump period. In this presentation, we analyze numerical simulations of prograde precession in accretion disks of CV SU UMa-like systems near superoutburst maximum. We compare the simulated positive superhump shapes with the shapes obtained from observations, using AW Sge as our model. Similarly, we compare associated Fourier Transforms with associated periodograms. We conclude with our analysis of the likely sources that generate the shape of the positive superhump in CV SU UMa-like systems near superoutburst maximum.

  20. Studies of frequency dependent C-V characteristics of neutron irradiated p+-n silicon detectors

    International Nuclear Information System (INIS)

    Frequency-dependent capacitance-voltage fluence (C-V) characteristics of neutron irradiated high resistivity silicon p+-n detectors have been observed up to a fluence of 8.0 x 1012 n/cm2. It has been found that frequency dependence of the deviation of the C-V characteristic (from its normal V-1/2 dependence), is strongly dependent on the ratio of the defect density and the effective doping density Nt/N'd. As the defect density approaches the effective dopant density, or Nt/N'd → 1, the junction capacitance eventually assumes the value of the detector geometry capacitance at high frequencies (f ≤ 105 Hz), independent of voltage. A two-trap-level model using the concept of quasi-fermi levels has been developed, which predicts both the effects of C-V frequency dependence and dopant compensation observed in this study

  1. 长虹佳华Zarva——CV100 MP4

    Institute of Scientific and Technical Information of China (English)

    Acwell

    2006-01-01

    长虹佳华CV100 MP4在功能上是款非常齐全的MP4。作为一款MP4产品我们从屏幕看起,CV100具有2英寸26万色LTPS LCD屏幕,输出效果很好,而这个屏幕还具有可视角度大,强光下表现好的特点,另外它还支持SD和MMC卡.这样就有足够大的空间来存储电影了。另一方面,CV100的定位不完全在MP4上。

  2. Storage of cut Heliconia bihai (L.) cv. Lobster Claw flowers at low temperatures Armazenamento de hastes florais de Heliconia bihai (L.) cv. Lobster Claw em baixa temperatura

    OpenAIRE

    Andreza S. Costa; Luis C. Nogueira; Venézio F. dos Santos; Terezinha R. Camara; Vivian Loges; Lilia Willadino

    2011-01-01

    The postharvest conservation of cut Heliconia flowers is an important factor to the success of commercialization, especially with regard to exportation. In the present study, the maximal storage time of cut inflorescences of Heliconia bihai cv. Lobster Claw at two different temperatures (12 and 19 °C) was evaluated and compared to laboratory conditions (25 °C, control treatment). Changes in visual quality, fresh weight and bract color (L*, a* and b*) were determined. The visual quality of the...

  3. Mineralogy and Petrography of MIL 090001, a Highly Altered CV Chondrite from the Reduced Sub-Group

    Science.gov (United States)

    Keller, Lindsay P.

    2011-01-01

    MIL 090001 is a large (greater than 6 kg) CV chondrite from the reduced subgroup (CV(sub red)) that was recovered during the 2009-2010 ANSMET field season [1]. The CV(sub red) subgroup meteorites retain primitive characteristics and have escaped the Na and Fe meta-somatism that affected the oxidized (CV(sub ox)) subgroups. MIL 090001 is, however, reported to be altered [1], and thus a major objective of this study is to characterize its mineralogy and petrography and the extent of the alteration.

  4. Khatyrka, a new CV3 find from the Koryak Mountains, Eastern Russia

    Science.gov (United States)

    MacPherson, Glenn J.; Andronicos, Christopher L.; Bindi, Luca; Distler, Vadim V.; Eddy, Michael P.; Eiler, John M.; Guan, Yunbin; Hollister, Lincoln S.; Kostin, Alexander; Kryachko, Valery; Steinhardt, William M.; Yudovskaya, Marina; Steinhardt, Paul J.

    2013-08-01

    A new meteorite find, named Khatyrka, was recovered from eastern Siberia as a result of a search for naturally occurring quasicrystals. The meteorite occurs as clastic grains within postglacial clay-rich layers along the banks of a small stream in the Koryak Mountains, Chukotka Autonomous Okrug of far eastern Russia. Some of the grains are clearly chondritic and contain Type IA porphyritic olivine chondrules enclosed in matrices that have the characteristic platy olivine texture, matrix olivine composition, and mineralogy (olivine, pentlandite, nickel-rich iron-nickel metal, nepheline, and calcic pyroxene [diopside-hedenbergite solid solution]) of oxidized-subgroup CV3 chondrites. A few grains are fine-grained spinel-rich calcium-aluminum-rich inclusions with mineral oxygen isotopic compositions again typical of such objects in CV3 chondrites. The chondritic and CAI grains contain small fragments of metallic copper-aluminum-iron alloys that include the quasicrystalline phase icosahedrite. One grain is an achondritic intergrowth of Cu-Al metal alloys and forsteritic olivine ± diopsidic pyroxene, both of which have meteoritic (CV3-like) oxygen isotopic compositions. Finally, some grains consist almost entirely of metallic alloys of aluminum + copper ± iron. The Cu-Al-Fe metal alloys and the alloy-bearing achondrite clast are interpreted to be an accretionary component of what otherwise is a fairly normal CV3 (oxidized) chondrite. This association of CV3 chondritic grains with metallic copper-aluminum alloys makes Khatyrka a unique meteorite, perhaps best described as a complex CV3 (ox) breccia.

  5. Python y OpenCV aplicados a un caso de estudio real

    OpenAIRE

    García del Arco, José Antonio

    2015-01-01

    Este trabajo se centra en el uso del lenguaje Python y la librería OpenCV de visión por computador para el seguimiento de crustáceos marinos en condiciones experimentales y determinar su comportamiento en un entorno social. Aquest treball es centra en l'ús del llenguatge Python i la llibreria OpenCV de visió per computador per al seguiment de crustacis marins en condicions experimentals i determinar el seu comportament en un entorn social.

  6. 论中脘穴的应用%Discussing Application of Zhongwan ( CV 12 ) Acupoint

    Institute of Scientific and Technical Information of China (English)

    张宪奇

    2011-01-01

    Based on the origins of literature, the modern theories research and clinical research, this paper reviewed and summarized the characteristic, function, indication and operation of Zhongwan ( CV 12 ), enriched the theory of experimental and clinical study on Zhongwan ( CV 12 ).%文章通过查阅相关理论文献及实验研究资料,并参考现代临床研究,详细论述了中脘的穴性、功能、主治以及操作,为中脘穴的实验及临床研究提供理论参考.

  7. Uso eficiente del agua para el cultivo de rosa cv. Freedom bajo invernadero

    OpenAIRE

    John J. Arévalo-H; Javier E. Vélez; Jesús H. Camacho-Tamayo

    2013-01-01

    Determinar el requerimiento de agua y el momento adecuado de distribución, es un factor fundamental para el cultivo de flores. El objetivo del presente trabajo fue determinar el efecto de la aplicación de tres láminas de riego sobre el desarrollo y producción del cultivo de la Rosa cv. Freedom, bajo invernadero. El experimento se realizó con tres láminas de riego en un cultivo de rosa (Rosa spp.) cv. Freedom. Se analizó el crecimiento de los tallos florales, la vida en florero, la producción ...

  8. STATE SPACE POINT DISTRIBUTION PARAMETER FOR SUPPORT VECTOR MACHINE BASED CV UNIT CLASSIFICATION

    Directory of Open Access Journals (Sweden)

    N K Narayanan

    2012-01-01

    Full Text Available In this paper we extend Support Vector Machines (SVM for speaker independent Consonant – Vowel (CV unit classification. Here we adopt the technique known as Decision Directed Acyclic Graph (DDAG , which is used to combine many two class classifiers into multiclass classifier. Using Reconstructed State Space (RSS based State Space Point Distribution (SSPD parameters, we obtain an average speaker independent phoneme recognition accuracy of 90% on the Malayalam V/CV speech unit database. The recognition results indicate that this method is efficient and can be adopted for developing a complete speech recognition system for Malayalam language.

  9. 一种高效的AAA下无对的无证书移动IP注册协议%Efficient mobile IP registration protocol with AAA using pairing-free certificateless public key cryptography

    Institute of Scientific and Technical Information of China (English)

    许捷; 党岚君; 石光明

    2014-01-01

    In order to realize the commercial deployment of the mobile IP, AAA (Authentication, Authorization,Accounting) is introduced into mobile IP registration.Aimed at the security and efficiency problems of the registration,a mobile IP registration protocol with AAA is proposed based on pairing-free certificateless public key cryptography.The mutual authentications between AAAF(AAA server in Foreign networks) and AAAH (AAA server in Home networks) are achieved by using a strongly secure and efficient certificateless signature scheme without pairings;the authentication of AAAH to MN (Mobile Node) and that of MN to HA (Home Agent) are realized via the MAC (Message Authentication Code). The user anonymity is achieved via the dynamic TID (Temporary Identity) instread of the user’s account information of the NAI(Network Access Identifier).Theoretical analyses show that the protocol reaches the expected security goals.Compared with the protocol proposed by Cao et al.,the protocol reduces the registration delay about 20% while improving the security.%为了实现移动 IP 的商业部署,需要将认证、授权和计费(Authentication, Authorization, Accounting,AAA)引入移动 IP注册过程。针对该注册过程安全与效率兼顾的问题,提出了 AAA下基于无对的无证书移动 IP注册协议。采用强安全高效的无对的无证书签名方案来实现外地 AAA 服务器与家乡AAA服务器之间的相互认证;采用消息认证码来实现家乡 AAA服务器对移动节点以及移动节点对家乡代理的认证。首次采用动态更新的临时身份来代替网络服务标识中的用户信息,实现了用户匿名性。分析结果表明,该协议达到了预期的安全目标。与Cao等人提出的协议相比,该协议具有更高的安全性,注册时延减少了约20%。

  10. Hairpin loops consisting of single adenine residues closed by sheared A.A and G.G pairs formed by the DNA triplets AAA and GAG: solution structure of the d(GTACAAAGTAC) hairpin.

    Science.gov (United States)

    Chou, S H; Zhu, L; Gao, Z; Cheng, J W; Reid, B R

    1996-12-20

    The DNA undecamers GTACAAAGTAC (AAA 11-mer) and GTACGAGGTAC (GAG 11-mer) have been studied in solution by high-resolution NMR spectroscopy. Both duplexes form stable hairpins containing single deoxyadenosine loops and stems containing five base-pairs that are closed at the loop end by sheared AxA and GxC pairs, respectively. These molecules thus contain new AAA and GAG loop turn motifs. All protons, including the chiral H5'/H5" protons of the loop residues, were assigned using NOESY, DQF-COSY and heteronuclear 1H-31P COSY experiments. The backbone torsion angles were constrained using experimental data from NOE crosspeaks, three-bond 1H-1H coupling constants and four-bond 1H-31P coupling constants and four-bond 1H-31P coupling constants. The AAA and GAG 11-mers form similar structures in solution. The detailed structure of the AAA 11-mer was determined by the combined use of NMR, distance geometry and energy minimization methods. This structure exhibits good stacking of the loop adenosine base on the closing 5Ax7A sheared pair, with the 6A base stacking on the 5A base and the 6A deoxyribose stacking with the 7A base. All sugars in the AAA 11-mer hairpin adopt the typical DNA C2'-endo conformation and a sharp backbone turn occurs between residues 6A and 7A. This loop turn is brought about mainly by a change in the backbone phosphate torsion angles from zeta(g-) alpha(g-) to zeta(g+) alphat(g+) at the turn. The gamma torsion angle of residue 7A in the closing sheared pair also changes from gauche+ to trans. In Pu1NPu2 loop turns of the GCA, AAA and GAG types, the chemical shift of the H4' proton of the loop deoxyribose depends on the nature of Pu2; this reflects the stacking of the loop sugar on the Pu2 base and the different ring current effects of A or G in this position. PMID:9000625

  11. AAA服务器在流媒体系统中的应用研究%Research on Application of AAA Server in the Streaming Media System.

    Institute of Scientific and Technical Information of China (English)

    李远英

    2013-01-01

    AAA refers to the athentication (authentication), authorization (Licensing), accounting (accounting). Since the birth of the network, authentication, authorization and accounting system have become the basis of the operation. The use of the resources on network, requires authentication, authorization and accounting to management. Confirm the identity of the user, according to the application when the user service category, system can grant customer permission. Finally, in the use of system resources to the user, need to have the appropriate equipment to statistical occupancy of resource users, according to the user to collect the corresponding costs[1]. The main work of the paper is to complete the AAA server design and in the RADIUS protocol, the RADIUS authentication/ authorized server structure design, through the analysis of the structure and further improvement, the test results show that the design and implementation of streaming media server structure can meet the real time management system, making the system accurate billing, carried on the test to the system and reached the purpose of this study.%  AAA指的是athentication(认证)、authorization(授权)、accounting(计费)。自网络诞生以来,认证、授权和计费体制就成为其运营的基础。网络中各类资源的使用,需要认证、授权和计费进行管理。在确认用户身份后,根据用户开户时所申请的服务类别,系统可以授予客户相应的权限。最后在用户使用系统资源时,需要有相应的设备来统计用户对资源的占用情况,据此向用户收取相应的费用[1]。论文的主要工作是完成AAA服务器的设计和在RADIUS协议下,设计RADIUS认证/授服务器结构,通过对这种结构的分析及进一步的改进,其测试结果表明服务器结构的设计和实现能够满足流媒体系统实时管理,使得系统计费准确,对系统进行了测试,基本达到研究目的。

  12. The CoxD protein, a novel AAA+ ATPase involved in metal cluster assembly: hydrolysis of nucleotide-triphosphates and oligomerization.

    Directory of Open Access Journals (Sweden)

    Tobias Maisel

    Full Text Available CoxD of the α-proteobacterium Oligotropha carboxidovorans is a membrane protein which is involved in the posttranslational biosynthesis of the [CuSMoO₂] cluster in the active site of the enzyme CO dehydrogenase. The bacteria synthesize CoxD only in the presence of CO. Recombinant CoxD produced in E. coli K38 pGP1-2/pETMW2 appeared in inclusion bodies from where it was solubilized by urea and refolded by stepwise dilution. Circular dichroism spectroscopy revealed the presence of secondary structural elements in refolded CoxD. CoxD is a P-loop ATPase of the AAA-protein family. Refolded CoxD catalyzed the hydrolysis of MgATP yielding MgADP and inorganic phosphate at a 1∶1∶1 molar ratio. The reaction was inhibited by the slow hydrolysable MgATP-γ-S. GTPase activity of CoxD did not exceed 2% of the ATPase activity. Employing different methods (non linear regression, Hanes and Woolf, Lineweaver-Burk, preparations of CoxD revealed a mean K(M value of 0.69±0.14 mM ATP and an apparent V(max value of 19.3±2.3 nmol ATP hydrolyzed min⁻¹ mg⁻¹. Sucrose density gradient centrifugation and gel filtration showed that refolded CoxD can exist in various multimeric states (2-mer, 4-mer or 6-mer, preferentially as hexamer or dimer. Within weeks the hexamer dissociates into the dimer, a process which can be reversed by MgATP or MgATP-γ-S within hours. Only the hexamers and the dimers exhibited MgATPase activity. Transmission electron microscopy of negatively stained CoxD preparations revealed distinct particles within a size range of 10-16 nm, which further corroborates the oligomeric organization. The 3D structure of CoxD was modeled with the 3D structure of BchI from Rhodobacter capsulatus as template. It has the key elements of an AAA+ domain in the same arrangement and at same positions as in BchI and displays the characteristic inserts of the PS-II-insert clade. Possible functions of CoxD in [CuSMoO₂] cluster assembly are discussed.

  13. Engagement of Arginine Finger to ATP Triggers Large Conformational Changes in NtrC1 AAA+ ATPase for Remodeling Bacterial RNA Polymerase

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Baoyu; Sysoeva, Tatyana A.; Chowdhury, Saikat; Guo, Liang; De Carlo, Sacha; Hanson, Jeffrey A.; Yang, Haw; Nixon, B. Tracy (IIT); (UCB); (City U/NY); (Penn)

    2010-11-19

    The NtrC-like AAA+ ATPases control virulence and other important bacterial activities through delivering mechanical work to {sigma}54-RNA polymerase to activate transcription from {sigma}54-dependent genes. We report the first crystal structure for such an ATPase, NtrC1 of Aquifex aeolicus, in which the catalytic arginine engages the {gamma}-phosphate of ATP. Comparing the new structure with those previously known for apo and ADP-bound states supports a rigid-body displacement model that is consistent with large-scale conformational changes observed by low-resolution methods. First, the arginine finger induces rigid-body roll, extending surface loops above the plane of the ATPase ring to bind {sigma}54. Second, ATP hydrolysis permits Pi release and retraction of the arginine with a reversed roll, remodeling {sigma}54-RNAP. This model provides a fresh perspective on how ATPase subunits interact within the ring-ensemble to promote transcription, directing attention to structural changes on the arginine-finger side of an ATP-bound interface.

  14. Differential roles of the COOH termini of AAA subunits of PA700 (19 S regulator) in asymmetric assembly and activation of the 26 S proteasome.

    Science.gov (United States)

    Gillette, Thomas G; Kumar, Brajesh; Thompson, David; Slaughter, Clive A; DeMartino, George N

    2008-11-14

    The 26 S proteasome is an energy-dependent protease that degrades proteins modified with polyubiquitin chains. It is assembled from two multi-protein subcomplexes: a protease (20 S proteasome) and an ATPase regulatory complex (PA700 or 19 S regulatory particle) that contains six different AAA family subunits (Rpt1 to -6). Here we show that binding of PA700 to the 20 S proteasome is mediated by the COOH termini of two (Rpt2 and Rpt5) of the six Rpt subunits that constitute the interaction surface between the subcomplexes. COOH-terminal peptides of either Rpt2 or Rpt5 bind to the 20 S proteasome and activate hydrolysis of short peptide substrates. Simultaneous binding of both COOH-terminal peptides had additive effects on peptide substrate hydrolysis, suggesting that they bind to distinct sites on the proteasome. In contrast, only the Rpt5 peptide activated hydrolysis of protein substrates. Nevertheless, the COOH-terminal peptide of Rpt2 greatly enhanced this effect, suggesting that proteasome activation is a multistate process. Rpt2 and Rpt5 COOH-terminal peptides cross-linked to different but specific subunits of the 20 S proteasome. These results reveal critical roles of COOH termini of Rpt subunits of PA700 in the assembly and activation of eukaryotic 26 S proteasome. Moreover, they support a model in which Rpt subunits bind to dedicated sites on the proteasome and play specific, nonequivalent roles in the asymmetric assembly and activation of the 26 S proteasome.

  15. Diverse pore loops of the AAA+ ClpX machine mediate unassisted and adaptor-dependent recognition of ssrA-tagged substrates.

    Science.gov (United States)

    Martin, Andreas; Baker, Tania A; Sauer, Robert T

    2008-02-29

    ClpX, an archetypal proteolytic AAA+ unfoldase, must engage the ssrA tags of appropriate substrates prior to ATP-dependent unfolding and translocation of the denatured polypeptide into ClpP for degradation. Here, specificity-transplant and disulfide-crosslinking experiments reveal that the ssrA tag interacts with different loops that form the top, middle, and lower portions of the central channel of the ClpX hexamer. Our results support a two-step binding mechanism, in which the top loop serves as a specificity filter and the remaining loops form a binding site for the peptide tag relatively deep within the pore. Crosslinking experiments suggest a staggered arrangement of pore loops in the hexamer and nucleotide-dependent changes in pore-loop conformations. This mechanism of initial tag binding would allow ATP-dependent conformational changes in one or more pore loops to drive peptide translocation, force unfolding, and mediate threading of the denatured protein through the ClpX pore.

  16. Oxygen isotope systematics of chondrules in the Allende CV3 chondrite: High precision ion microprobe studies

    Digital Repository Service at National Institute of Oceanography (India)

    Rudraswami, N.G.; Ushikubo, T.; Nakashima, D.; Kita, N.T.

    in local dust-rich protoplanetary disk, from which the CV3 parent asteroid formed. A compilation of 225 olivine and low-Ca pyroxene isotopic data from 36 chondrules analyzed in the present study lie between carbonaceous chondrite anhydrous mineral (CCAM...

  17. Indirect measurements of Brachiaria brizantha cv. Marandu fermentable cell wall sugars for second generation biofuels production.

    Science.gov (United States)

    Results of a study conducted to evaluate the possibility of using IVDMD values of B. brizantha cv. Marandu to predict cell wall sugars that would be available in a biorefinery for ethanol production are reported. The study was conducted based on the similarity between rumen enzymes and those used i...

  18. Abstract Morphemes and Lexical Representation: The CV-Skeleton in Arabic

    Science.gov (United States)

    Boudelaa, Sami; Marslen-Wilson, Willian D.

    2004-01-01

    Overlaps in form and meaning between morphologically related words have led to ambiguities in interpreting priming effects in studies of lexical organization. In Semitic languages like Arabic, however, linguistic analysis proposes that one of the three component morphemes of a surface word is the CV-Skeleton, an abstract prosodic unit coding the…

  19. Automatic tracking of red blood cells in micro channels using OpenCV

    Science.gov (United States)

    Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

    2013-10-01

    The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

  20. Cherry Picking Robot Vision Recognition System Based on OpenCV

    Directory of Open Access Journals (Sweden)

    Zhang Qi Rong

    2016-01-01

    Full Text Available Through OpenCV function, the cherry in a natural environment image after image preprocessing, color recognition, threshold segmentation, morphological filtering, edge detection, circle Hough transform, you can draw the cherry’s center and circular contour, to carry out the purpose of the machine picking. The system is simple and effective.

  1. Cherry Picking Robot Vision Recognition System Based on OpenCV

    OpenAIRE

    Zhang Qi Rong; Peng Pei; Jin Yan Mei

    2016-01-01

    Through OpenCV function, the cherry in a natural environment image after image preprocessing, color recognition, threshold segmentation, morphological filtering, edge detection, circle Hough transform, you can draw the cherry’s center and circular contour, to carry out the purpose of the machine picking. The system is simple and effective.

  2. Galaxy and Mass Assembly (GAMA): The GAMA Galaxy Group Catalogue (G3Cv1)

    CERN Document Server

    Robotham, A S G; Driver, S P; Baldry, I K; Bamford, S P; Hopkins, A M; Liske, J; Loveday, J; Merson, A; Peacock, J A; Brough, S; Cameron, E; Conselice, C J; Croom, S M; Frenk, C S; Gunawardhana, M; Hill, D T; Jones, D H; Kelvin, L S; Kuijken, K; Nichol, R C; Parkinson, H R; Pimbblet, K A; Phillipps, S; Popescu, C C; Prescott, M; Sharp, R G; Sutherland, W J; Taylor, E N; Thomas, D; Tuffs, R J; van Kampen, E; Wijesinghe, D

    2011-01-01

    Using the complete GAMA-I survey covering ~142 sq. deg. to r=19.4, of which ~47 sq. deg. is to r=19.8, we create the GAMA-I galaxy group catalogue (G3Cv1), generated using a friends-of-friends (FoF) based grouping algorithm. Our algorithm has been tested extensively on one family of mock GAMA lightcones, constructed from Lambda-CDM N-body simulations populated with semi-analytic galaxies. Recovered group properties are robust to the effects of interlopers and are median unbiased in the most important respects. G3Cv1 contains 14,388 galaxy groups (with multiplicity >= 2$), including 44,186 galaxies out of a possible 110,192 galaxies, implying ~40% of all galaxies are assigned to a group. The similarities of the mock group catalogues and G3Cv1 are multiple: global characteristics are in general well recovered. However, we do find a noticeable deficit in the number of high multiplicity groups in GAMA compared to the mocks. Additionally, despite exceptionally good local spatial completeness, G3Cv1 contains signif...

  3. The influence of virus diseases on grape polyphenols of cv. 'Refosk'

    International Nuclear Information System (INIS)

    External stimuli such as microbial infections, ultraviolet radiation, and chemical stressors can modulate the synthesis of polyphenols in the plants. Cv. 'Refosk' was used to show the influence of the GLRaV-1 and rugose wood (RW) on the polyphenols in grape. The infection shifted polyphenols from seeds to grape skins but had no impact on anthocyanins

  4. VizieR Online Data Catalog: 72 faint CV candidates in CRTS (Breedt+, 2014)

    Science.gov (United States)

    Breedt, E.; Gansicke, B. T.; Drake, A. J.; Rodriguez-Gil, P.; Parsons, S. G.; Marsh, T. R.; Szkody, P.; Schreiber, M. R.; Djorgovski, S. G.

    2016-04-01

    We obtained identification spectra of a total of 72 faint CV candidates identified by the CRTS, using the Gran Telescopio Canarias (GTC; La Palma, Spain) and the Gemini telescopes (North: Mauna Kea, Hawaii and South: Cerro Pachon, Chile). The observations were carried out in service mode during 2010, 2011 and 2013. (5 data files).

  5. Endogenous auxin regulates the sensitivity of Dendrobium (cv. Miss Teen) flower pedicel abscission to ethylene

    NARCIS (Netherlands)

    Rungruchkanont, K.; Ketsa, S.; Chatchawankanphanich, O.; Doorn, van W.G.

    2007-01-01

    Dendrobium flower buds and flowers have an abscission zone at the base of the pedicel (flower stalk). Ethylene treatment of cv. Miss Teen inflorescences induced high rates of abscission in flower buds but did not affect abscission once the flowers had opened. It is not known if auxin is a regulator

  6. Isolation and characterization of CvIV4: a pain inducing α-scorpion toxin.

    Directory of Open Access Journals (Sweden)

    Ashlee H Rowe

    Full Text Available BACKGROUND: Among scorpion species, the Buthidae produce the most deadly and painful venoms. However, little is known regarding the venom components that cause pain and their mechanism of action. Using a paw-licking assay (Mus musculus, this study compared the pain-inducing capabilities of venoms from two species of New World scorpion (Centruroides vittatus, C. exilicauda belonging to the neurotoxin-producing family Buthidae with one species of non-neurotoxin producing scorpion (Vaejovis spinigerus in the family Vaejovidae. A pain-inducing α-toxin (CvIV4 was isolated from the venom of C. vittatus and tested on five Na(+ channel isoforms. PRINCIPAL FINDINGS: C. vittatus and C. exilicauda venoms produced significantly more paw licking in Mus than V. spinigerus venom. CvIV4 produced paw licking in Mus equivalent to the effects of whole venom. CvIV4 slowed the fast inactivation of Na(v1.7, a Na(+ channel expressed in peripheral pain-pathway neurons (nociceptors, but did not affect the Na(v1.8-based sodium currents of these neurons. CvIV4 also slowed the fast inactivation of Na(v1.2, Na(v1.3 and Na(v1.4. The effects of CvIV4 are similar to Old World α-toxins that target Na(v1.7 (AahII, BmK MI, LqhIII, OD1, however the primary structure of CvIV4 is not similar to these toxins. Mutant Na(v1.7 channels (D1586A and E1589Q, DIV S3-S4 linker reduced but did not abolish the effects of CvIV4. CONCLUSIONS: This study: 1 agrees with anecdotal evidence suggesting that buthid venom is significantly more painful than non-neurotoxic venom; 2 demonstrates that New World buthids inflict painful stings via toxins that modulate Na(+ channels expressed in nociceptors; 3 reveals that Old and New World buthids employ similar mechanisms to produce pain. Old and New World α-toxins that target Na(v1.7 have diverged in sequence, but the activity of these toxins is similar. Pain-inducing toxins may have evolved in a common ancestor. Alternatively, these toxins may be the

  7. NASA Aircraft on ramp (Aerial view) Sides: (L) QSRA (R) C-8A AWJSRA - Back to Front: CV-990 (711)

    Science.gov (United States)

    1981-01-01

    NASA Aircraft on ramp (Aerial view) Sides: (L) QSRA (R) C-8A AWJSRA - Back to Front: CV-990 (711) C-141 KAO, CV-990 (712) Galileo, T-38, YO-3A, Lear Jet, X-14, U-2, OH-6, CH-47, SH-3G, RSRA, AH-1G, XV-15, UH-1H

  8. Beryllium-Boron Systematics of Refractory Inclusions in CR2 and CV3 Chondrites: Evidence for 10Be Heterogeneity

    Science.gov (United States)

    Dunham, E.; Wadhwa, M.; Simon, S.; Grossman, L.

    2016-08-01

    Be-B systematics of Allende (CV3), Axtell (CV3), and NWA 5028 (CR2) CAIs suggests that 10Be was distributed heterogeneously in the early solar system which implies that 10Be was produced in the solar nebula by irradiation of nebular gas or dust.

  9. [The estimation of daily physical activity with the coefficient of variation (CV) of heart rates continuously recorded].

    Science.gov (United States)

    Tono-oka, T; Kaneko, I

    1993-05-01

    The daily level of physical activity was estimated using the heart rate monitor, PE3000 (Polar Electro, Finland). The level was expressed with the coefficient of variation (CV) of heart rates recorded from waking time to dinner time. In the course of a day of intense physical activity, CV was confirmed to rise significantly. Then the CV was estimated and compared among 3 age classes, young (10-18 years), middle-aged (30-47 years), and elderly (62-76 years). The CVs of young people were significantly higher than those of middle-aged (P < 0.001) and elderly (P < 0.01), regardless of sex. However there was no significant sex difference in all age classes. These results suggest that the CV is an accurate index of daily physical activity. Thus clinicians can use the CV of heart rates to estimate the level of physical activity of individuals which closely relates to QOL.

  10. Comportamento agronômico inicial da cv. Chimarrita enxertada em cinco porta-enxertos de pessegueiro Agronomical behavior of cv. Chimarrita on five rootstocks of peach trees

    Directory of Open Access Journals (Sweden)

    Moacir da Silva Rocha

    2007-01-01

    Full Text Available O trabalho teve por objetivo avaliar a resposta agronômica da cv. Chimarrita enxertada em cinco porta-enxertos, nas condições edafoclimáticas da região de Pelotas-RS, no período de 2003 a 2005. Durante o período de execução do experimento, foram avaliados o diâmetro do tronco do porta-enxerto e da cultivar-copa, comprimento médio dos ramos principais, volume de copa, massa fresca e massa seca do material vegetal retirado nas podas verde e de inverno, índice de intensidade de poda, massa média dos frutos, produção por planta, eficiência produtiva, produção por hectare, sólidos solúveis totais, firmeza da polpa, diâmetro e coloração dos frutos. O porta-enxerto 'Capdeboscq' induziu o maior crescimento vegetativo na cv. Chimarrita durante os três anos de avaliação, seguido do porta-enxerto 'Okinawa'. Este, por sua vez, induziu o maior rendimento produtivo (1,65 t ha-1. A cv. Capdeboscq proporcionou a obtenção de frutos com maior massa. Os porta-enxertos 'GF 305' e 'Aldrighi' induziram menor desenvolvimento vegetativo e a mais baixa produtividade (0,52 t.ha-1 .The objective for this study was to evaluate the agronomical behavior of cv. Chimarrita on five rootstocks of peach trees in Pelotas- RS from 2003 to 2005. The variables evaluated were: trunk diameters of the rootstock and of the scion; length of the annual terminal growth; volume of the canopy; fresh and dry weight of the material taken by the summer and winter pruning; pruning intensity index; fruit weight; fruit production per tree; production efficiency; productivity per hectare; total soluble solids; pulp firmness; and fruit diameter and color. It was observed that the trees from: rootstock 'Capdeboscq' promoted higher vegetative growth and larger fruits, followed by the ones from the rootstock 'Okinawa' which promoted the highest yield (1, 65 t.ha-1; the rootstock 'GF 305' and 'Aldrighi' had the lowest vegetative development and yield (0, 52 t.ha-1.

  11. Storage of cut Heliconia bihai (L. cv. Lobster Claw flowers at low temperatures Armazenamento de hastes florais de Heliconia bihai (L. cv. Lobster Claw em baixa temperatura

    Directory of Open Access Journals (Sweden)

    Andreza S. Costa

    2011-09-01

    Full Text Available The postharvest conservation of cut Heliconia flowers is an important factor to the success of commercialization, especially with regard to exportation. In the present study, the maximal storage time of cut inflorescences of Heliconia bihai cv. Lobster Claw at two different temperatures (12 and 19 °C was evaluated and compared to laboratory conditions (25 °C, control treatment. Changes in visual quality, fresh weight and bract color (L*, a* and b* were determined. The visual quality of the inflorescences and fresh weight decreased with time in all treatments. Symptoms of chilling injury were observed on the inflorescences stored at 12 °C for six and eight days. Bract color was not affected by temperature, storage time or the senescence process. The results indicate that a temperature of 12 °C is not recommended for a storage time longer than four days, whereas 19 °C can be used for a storage time of up to eight days for cut inflorescences of H. bihai cv. Lobster Claw.A conservação pós-colheita de flores de corte de Heliconia é fator relevante para o sucesso da comercialização, principalmente para a exportação. Neste estudo, o período máximo de armazenamento de hastes florais de Heliconia bihai cv. Lobster Claw, foi avaliado em duas diferentes temperaturas (12 e 19 °C e comparado com as condições de laboratório (25 °C, tratamento controle. As variáveis avaliadas foram: qualidade visual, massa de matéria fresca e a coloração das inflorescências (L*, a* e b*. A qualidade visual das inflorescências e a massa de matéria fresca de todos os tratamentos reduziram ao longo do tempo. Sintomas de injúria por frio foram observados nas inflorescências armazenadas a 12 °C, durante seis e oito dias. A coloração das brácteas não foi afetada pela temperatura, período de armazenamento nem pelo processo de senescência. Os resultados indicam que a temperatura de 12 °C não é recomendada para armazenar hastes florais de

  12. 資産負債中心主義と収益費用中心主義の比較 -FASB概念ステートメント,会社会計基準序説,AAA1957年会計原則-

    OpenAIRE

    岡田, 裕正

    1995-01-01

    This paper compares the accounting structures in the FASB Conceptual Statements, the Paton-Littleton's "An Introduction to Corporate Accounting Standards" and the AAA's Accounting Standard in 1957. As many researchers say, FASB's Conceptual Statements has Asset-and-Liability View and Paton-Littleton's monograph has Revenue-and-Expense View. These two have different accounting structure. And this paper also clarifies that the accounting structure of the AAA's standard is placed between Asset-a...

  13. PBC算法与AAA算法在肺癌调强放疗中的剂量学比较%Dosimetric Comparison of PBC and AAA Dose Calculation Algorithm for IMRT Treatment of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    张富利; 王军良; 盛洪国; 郑明民

    2011-01-01

    Objective: To compare the dosimetric differences between intensity modulated radiotherapy (IMRT) plans for non-small cell lung cancer using pencil beam convolution (PBC) and anisotropic analytical algorithm (AAA) methods.Materials and Methods: For a cohort of 7 patients, IMRT plans were designed using PBC and AAA calculation methods, respectively.In the planning process, identical planning objectives and dose constraints were set.Plans were compared according to dose-volume histogram (DVH) analysis in terms of PTV homogeneity and conformity indices (HI and CI) as well as OARs dose and volume parameters.Results: On average, no statistically significant differences between PBC and AAA plans were observed except Dmean and VI0 of lung.Conclusions: Both PBC and AAA algorithms provide similar dose computation accuracy for NSCLC,but the latter is less affected by the variation of respiratory phase and suggested as a priority choice.%目的:分析、比较笔形束卷积算法(PBC)和各向异性解析算法(AAA)在非小细胞肺癌(NSCLC)调强放疗计划设计中的剂量学差异.方法:随机选择7例NSCLC患者,采用Eclipse version 7.3.10计划系统提供的PBC算法和AAA算法对每例NSCLC进行IMRT的计划设计,比较靶区及危及器官的剂量分布、DVH等指标.结果:两种算法获得治疗计划的靶区剂量均匀性和适形度均无明显差别,食管、心脏、脊髓等危及器官的受量也基本相同.结论:对于NSCLC,剂量计算应采用受呼吸时相影响更小的AAA算法.

  14. Fox grape cv. Bordô (Vitis labrusca L. and grapevine cv. Chardonnay (Vitis vinifera L. cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels

    Directory of Open Access Journals (Sweden)

    Dayse Cristina de Carvalho

    2013-04-01

    Full Text Available The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the cultivars. Bordô and Chardonnay had different amino acid preferences for some parameters. In conclusion, for in vitro shoot regeneration from the nodal segments, culture on solid medium with 5 μM BAP, 15 g.L-1 sucrose for Bordô and 45 g.L-1 sucrose for Chardonnay showed better results. Similarly, the supplementation of 40 g.L-1 arginine for Bordô and 40 g.L-1 arginine or glycine for Chardonnay showed better results.

  15. Formation of biphenyl and dibenzofuran phytoalexins in the transition zones of fire blight-infected stems of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference'.

    Science.gov (United States)

    Chizzali, Cornelia; Khalil, Mohammed N A; Beuerle, Till; Schuehly, Wolfgang; Richter, Klaus; Flachowsky, Henryk; Peil, Andreas; Hanke, Magda-Viola; Liu, Benye; Beerhues, Ludger

    2012-05-01

    In the rosaceous subtribe Pyrinae (formerly subfamily Maloideae), pathogen attack leads to formation of biphenyls and dibenzofurans. Accumulation of these phytoalexins was studied in greenhouse-grown grafted shoots of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference' after inoculation with the fire blight bacterium, Erwinia amylovora. No phytoalexins were found in leaves. However, both classes of defence compounds were detected in the transition zone of stems. The flanking stem segments above and below this zone, which were necrotic and healthy, respectively, were devoid of detectable phytoalexins. The transition zone of apple stems contained the biphenyls 3-hydroxy-5-methoxyaucuparin, aucuparin, noraucuparin and 2'-hydroxyaucuparin and the dibenzofurans eriobofuran and noreriobofuran. In pear, aucuparin, 2'-hydroxyaucuparin, noreriobofuran and in addition 3,4,5-trimethoxybiphenyl were detected. The total phytoalexin content in the transition zone of pear was 25 times lower than that in apple. Leaves and stems of mock-inoculated apple and pear shoots lacked phytoalexins. A number of biphenyls and dibenzofurans were tested for their in vitro antibacterial activity against some Erwinia amylovora strains. The most efficient compound was 3,5-dihydroxybiphenyl (MIC=115 μg/ml), the immediate product of biphenyl synthase which initiates phytoalexin biosynthesis.

  16. 基于 OpenCV 的双目摄像机标定技术研究%Binocular Camera Calibration Technique Based on OpenCV

    Institute of Scientific and Technical Information of China (English)

    王长元; 邢世蒙

    2014-01-01

    As a foundational research in the field of binocular stereo vision ,camera calibration technique has great theo‐retical value and practical value .This paper focuses on the binocular stereo vision camera calibration technique to analyze the binocular camera calibration method based on OpenCV .OpenCV camera calibration based on binocular is more flexible than traditional scaling techniques ,and has a good calibration accuracy .%摄像机标定技术作为双目立体视觉领域的一个基础性研究课题,具有很大的理论研究价值和实际应用价值。论文围绕双目立体视觉中的双目摄像机标定技术,分析基于OpenCV的双目摄像机标定的方法。基于OpenCV的双目摄像机标定比传统的定标技术更为灵活,并且具有不错的定标精度。

  17. 基于 OpenCV 的双目摄像机标定技术研究%Binocular Camera Calibration Technique Based on OpenCV

    Institute of Scientific and Technical Information of China (English)

    王长元; 邢世蒙

    2014-01-01

    摄像机标定技术作为双目立体视觉领域的一个基础性研究课题,具有很大的理论研究价值和实际应用价值。论文围绕双目立体视觉中的双目摄像机标定技术,分析基于OpenCV的双目摄像机标定的方法。基于OpenCV的双目摄像机标定比传统的定标技术更为灵活,并且具有不错的定标精度。%As a foundational research in the field of binocular stereo vision ,camera calibration technique has great theo‐retical value and practical value .This paper focuses on the binocular stereo vision camera calibration technique to analyze the binocular camera calibration method based on OpenCV .OpenCV camera calibration based on binocular is more flexible than traditional scaling techniques ,and has a good calibration accuracy .

  18. Avaliação da Brachiaria brizantha cv. marandu em sistemas silvipastoris Evaluation of Brachiaria brizantha cv. marandu in silvopastoral systems

    Directory of Open Access Journals (Sweden)

    G.R. Moreira

    2009-06-01

    Full Text Available Avaliou-se a degradabilidade in situ da matéria seca (MS da Brachiaria brizantha cv. marandu colhida em dois sistemas silvipastoris compostos pelas arbóreas, ipê felpudo (Zeyheria tuberculosa e aroeira (Myracrodruon urundeuva, localizados no bioma Cerrado, município de Lagoa Santa, MG. O período de coleta da forragem foi de dezembro de 2004 a abril de 2005. O delineamento utilizado foi em blocos ao acaso, segundo esquema de parcelas subdivididas, sendo as parcelas os tratamentos e as sub-parcelas os tempos de degradação. O sombreamento com as arbóreas não influenciou a degradabilidade in situ da matéria seca da forrageira.In situ degradability of dry matter of Brachiaria brizantha cv. marandu harvested in two silvopastoral systems was evaluated. One system was composed by Zeyheria tuberculosa tree and the other by Myracrodruon urundeuva, both located at Brazilian Savannah, Lagoa Santa, MG. A completely ramdomized block experimental design in a splitplot arrangement was used. The treatments were allocated in the plots and the times in the splitplot. No effect of shade was observed for in situ dry matter degradability.

  19. NVL2, a nucleolar AAA-ATPase, is associated with the nuclear exosome and is involved in pre-rRNA processing

    Energy Technology Data Exchange (ETDEWEB)

    Yoshikatsu, Yuki [Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima 770-8506 (Japan); Ishida, Yo-ichi; Sudo, Haruka [Department of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo 204-8588 (Japan); Yuasa, Keizo; Tsuji, Akihiko [Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima 770-8506 (Japan); Nagahama, Masami, E-mail: nagahama@my-pharm.ac.jp [Department of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo 204-8588 (Japan)

    2015-08-28

    Nuclear VCP-like 2 (NVL2) is a member of the chaperone-like AAA-ATPase family and is involved in the biosynthesis of 60S ribosomal subunits in mammalian cells. We previously showed the interaction of NVL2 with a DExD/H-box RNA helicase MTR4/DOB1, which is a known cofactor for an exoribonuclease complex, the exosome. This finding implicated NVL2 in RNA metabolic processes during ribosome biogenesis. In the present study, we found that a series of mutations within the ATPase domain of NVL2 causes a defect in pre-rRNA processing into mature 28S and 5.8S rRNAs. Co-immunoprecipitation analysis showed that NVL2 was associated with the nuclear exosome complex, which includes RRP6 as a nucleus-specific catalytic subunit. This interaction was prevented by depleting either MTR4 or RRP6, indicating their essential role in mediating this interaction with NVL2. Additionally, knockdown of MPP6, another cofactor for the nuclear exosome, also prevented the interaction by causing MTR4 to dissociate from the nuclear exosome. These results suggest that NVL2 is involved in pre-rRNA processing by associating with the nuclear exosome complex and that MPP6 is required for maintaining the integrity of this rRNA processing complex. - Highlights: • ATPase-deficient mutants of NVL2 have decreased pre-rRNA processing. • NVL2 associates with the nuclear exosome through interactions with MTR4 and RRP6. • MPP6 stabilizes MTR4-RRP6 interaction and allows NVL2 to interact with the complex.

  20. Comportamento de Andropogon gayanus cv. ‘planaltina’ e Panicum maximum cv. ‘tanzânia’ sob sombreamento Performance of Andropogon gayanus and Panicum maximum cv. ‘Tanzania’ in the shading

    Directory of Open Access Journals (Sweden)

    Fabiana Lopes Ramos de Oliveira

    2013-02-01

    Full Text Available A utilização de forrageiras tolerantes ao sombreamento é fundamental para a sustentabilidade da integração lavoura-pecuária-floresta. Estudou-se o comportamento do Panicum maximum cv. 'Tanzânia' e do Andropogon gayanus cv. 'Planaltina' sob diferentes níveis de sombreamentos e épocas de entrada sob a sombra. Utilizou-se delineamento em blocos casualizados, com quatro repetições, em esquema fatorial com dois tipos de sombreamento: 30% e 50%; três épocas de entrada na sombra após transplante (07, 14 e 21 dias; e uma testemunha, mantida a pleno sol. Mudas com 8 a 12cm de altura das duas espécies, pré-cultivadas em bandejas de isopor, foram transplantadas para vasos e mantidas nos respectivos ambientes sombreados ou a pleno sol por 90 dias, em livre crescimento. A época de entrada na sombra não interferiu na produtividade e nos teores de clorofila foliar das cultivares, assim como na composição bromatológica. As massas seca e fresca das cultivares 'Tanzânia' e 'Planaltina' foram superiores em ambientes sombreados, quando comparadas ao pleno sol. As duas forrageiras, quando sombreadas, incrementaram os teores de clorofila a, porém não se observou alteração para clorofila b. A adaptação positiva em ambientes sombreados das cultivares torna-se promissora em sistemas de integração silvipastoris.The use of grasses tolerant to shade is essential to the sustainability of the Farming-Livestock-Forest Integration System. The performance of Panicum maximum cv. 'Tanzania' and Andropogon gayanus cv. 'Planaltina' under different levels of shading and times of entry under the shade were studied. We used designs in randomized blocks with four replications in a factorial scheme with two types of shading: 30% and 50%; three times of entry into the shade after transplantation (07, 14 and 21 days and a witness, kept in full sun. Seedlings 8 to 12cm high of both species, pre-cultivated in styrofoam trays were transplanted to pots and

  1. Modificação da atmosfera na qualidade pós-colheita de ameixas cv. Reubennel Modified atmosphere on postharvest quality of plums cv. Reubennel

    Directory of Open Access Journals (Sweden)

    Marcelo Barbosa Malgarim

    2005-12-01

    Full Text Available Ameixas, de modo geral, têm curto período de conservação pós-colheita, havendo necessidade de otimizar as condições de colheita e de armazenamento. Este trabalho objetivou avaliar o efeito de diferentes modificadores de atmosfera, durante o armazenamento refrigerado, na qualidade pós-colheita de ameixas cv. Reubennel. Frutas no estádio de maturação meio-maduro foram submetidas aos seguintes modificadores de atmosfera: filmes de polietileno de 12,5 e 15µm de espessura e cera à base de carnaúba e avaliadas após 10; 20; 30 e 40 dias de armazenamento a 0ºC e UR de 90-95%. Avaliaram-se a perda de massa, cor, firmeza da polpa, pH, sólidos solúveis (SS, acidez titulável (AT, relação SS/AT, incidência de podridões, escurecimento interno e características sensoriais. Essas avaliações foram realizadas 3 dias após a retirada das frutas da câmara fria. Verificou-se que a cera à base de carnaúba GARFRESH N (BL9,5, sem diluição, e filmes de polietileno de 12,5 e 15µm reduziram a perda de massa, porém são impróprios para a modificação da atmosfera, em ameixas cv. Reubennel durante o armazenamento refrigerado. Ameixas cv. Reubennel mantêm a qualidade quando armazenadas durante 30 dias a 0ºC sem atmosfera modificada.Plums quickly loose postharvest quality, which makes imperative to optimize harvest and storage conditions. The objective of this work was to determine the effects of various atmosphere modifiers on the postharvest quality of cold storage plums, cv. Reubennel. Semi-mature fruits were stored under the following atmosphere modifiers conditions: polyethylene films 12,5 or 15µm of thickness; or carnauba wax. After submitted to these conditions the fruits were stored for 10, 20, 30 and 40 days in a cold room at 0ºC and 90-95% RH. Three days after taken out from the cold storage, the fruits were evaluated about: weight loss; color; firmness; pH; soluble solids (SS; titratable acidity (TA; SS/TA ratio; incidence of

  2. Efeito de reguladores de crescimento em uva apirênica, cv. BRS Clara Effect of growth regulators on the seedless grape cv. BRS Clara

    Directory of Open Access Journals (Sweden)

    Jair Costa Nachtigal

    2005-08-01

    Full Text Available O presente trabalho teve por objetivo verificar o efeito da aplicação de diferentes concentrações do ácido giberélico (AG3, do thidiazuron (TDZ e do forchlorfenuron (CPPU no aumento do tamanho dos cachos e das bagas e no teor de sólidos solúveis totais de uva sem semente, cv. BRS Clara. As plantas foram conduzidas no sistema de latada, sobre o porta-enxerto IAC 572, no espaçamento 2,5 x 2,0m e com irrigação por microaspersão. Os experimentos foram conduzidos na Estação Experimental de Viticultura Tropical, da Embrapa Uva e Vinho, em Jales-SP. Os reguladores de crescimento foram aplicados via pulverização localizada no cacho, utilizando-se de concentrações de 0 a 4mg.L-1 de CPPU; 0 a 10mg.L-1 de TDZ, e de 0 a 90mg.L-1 de AG3, isolados ou em conjunto. De modo geral, a aplicação dos reguladores de crescimento promove a melhoria da qualidade dos cachos da cv. BRS Clara; o uso do TDZ e do CPPU em conjunto com o AG3 produz um efeito sinérgico, proporcionando melhor resposta do que o uso isolado do AG3; os tratamentos com 60mg.L-1 de AG3; 20mg.L-1 de AG3 + 4mg.L-1 de CPPU, e 10mg.L-1 de AG3 + 5mg.L-1 de TDZ proporcionam os melhores resultados para o aumento do diâmetro das bagas; a aplicação de AG3 antes da floração da cv. BRS Clara provoca abortamento excessivo, reduzindo a qualidade comercial dos cachos; a utilização de concentrações elevadas dos reguladores reduz o teor de sólidos solúveis totais.The objective of this study was to evaluate the effect of different concentrations of gibberellic acid (AG3, thidiazuron (TDZ and forchlorfenuron (CPPU on the size of bunches and berries and on the total soluble solids content, when applied in different concentrations on the seedless grapes, cv. BRS Clara. The grapevines were grafted on the rootstock IAC 572, trained in the pergola system and, spaced 2.5 x 2.0m and irrigated by micro sprinklers. The experiments were carried out at the Embrapa Grape and Wine Experimental

  3. A REAL-TIME C-V CLUSTERING ALGORITHM FOR WEB-MINING

    Institute of Scientific and Technical Information of China (English)

    Li Haiying; Zhuang Zhenquan; Li Bin; Wan Ke

    2002-01-01

    In this letter, a real-time C-V (Characteristic-Vector) clustering algorithm is put forth to treat with vast action data which are dynamically collected from web site. The algorithm cites the concept of C-V to denote characteristic, synchronously it adopts two-value [0,1]input and self-definition vigilance parameter to design clustering-architecture. Vector Degree of Matching (VDM) plays a key role in the clustering algorithm, which determines the magnitude of typical characteristic. Making use of stability analysis, the classifications are confirmed to have reliably hierarchical structure when vigilance parameter shifts from 0.1 to 0.99. This non-linear relation between vigilance parameter and classification upper limit helps mining out representative classifications from net-users according to the actual web resource, then administering system can map them to web resource space to implement the intelligent configuration effectually and rapidly.

  4. Potato cv. Romano reaction to primary and secondary infection with potato necrotic strain Y virus (PVYNTN

    Directory of Open Access Journals (Sweden)

    Drago Milošević

    2015-01-01

    Full Text Available Primary and secondary infections with PVYNTN were investigated on forty plants of the potato cv. Romano inoculated in a greenhouse in Serbia in 2012 and 2013. PVY isolates were collected from the potato growing region of Čačak and identified by ELISA and RT-PCR methods. The sequence of the Serbian isolate 3D (Acc. No. KJ946936 showed 100% match with seven PVY isolates deposited in GenBank and described as NTN. A significant difference was detected between PVYNTN symptoms exibited on leaves of the cv. Romano under primary and secondary infections. The findings are significant because they are based on symptoms observed, so that it is clear that there are two distinct types of infection: primary and secondary. Symptoms of primary and secondary infection were the same on potato tubers and had the form of necrotic rings.

  5. Camera calibration method of binocular stereo vision based on OpenCV

    Science.gov (United States)

    Zhong, Wanzhen; Dong, Xiaona

    2015-10-01

    Camera calibration, an important part of the binocular stereo vision research, is the essential foundation of 3D reconstruction of the spatial object. In this paper, the camera calibration method based on OpenCV (open source computer vision library) is submitted to make the process better as a result of obtaining higher precision and efficiency. First, the camera model in OpenCV and an algorithm of camera calibration are presented, especially considering the influence of camera lens radial distortion and decentering distortion. Then, camera calibration procedure is designed to compute those parameters of camera and calculate calibration errors. High-accurate profile extraction algorithm and a checkboard with 48 corners have also been used in this part. Finally, results of calibration program are presented, demonstrating the high efficiency and accuracy of the proposed approach. The results can reach the requirement of robot binocular stereo vision.

  6. Diagnosis of Elevator Faults with LS-SVM Based on Optimization by K-CV

    Directory of Open Access Journals (Sweden)

    Zhou Wan

    2015-01-01

    Full Text Available Several common elevator malfunctions were diagnosed with a least square support vector machine (LS-SVM. After acquiring vibration signals of various elevator functions, their energy characteristics and time domain indicators were extracted by theoretically analyzing the optimal wavelet packet, in order to construct a feature vector of malfunctions for identifying causes of the malfunctions as input of LS-SVM. Meanwhile, parameters about LS-SVM were optimized by K-fold cross validation (K-CV. After diagnosing deviated elevator guide rail, deviated shape of guide shoe, abnormal running of tractor, erroneous rope groove of traction sheave, deviated guide wheel, and tension of wire rope, the results suggested that the LS-SVM based on K-CV optimization was one of effective methods for diagnosing elevator malfunctions.

  7. Reconstructed State Space Model for Recognition of Consonant - Vowel (Cv Utterances Using Support Vector Machines

    Directory of Open Access Journals (Sweden)

    N K Narayanan

    2012-04-01

    Full Text Available This paper presents a study on the use of Support Vector Machines (SVMs in classifying Malayalam Consonant – Vowel (CV speech unit by comparing it to two other classification algorithms namely Artificial Neural Network (ANN and k – Nearest Neighbourhood (k – NN. We extend SVM to combine many two class classifiers into multiclass classifier using Decision Directed Acyclic Graph (DDAG algorithm. A feature extraction technique using Reconstructed State Space(RSS based State Space PointDistribution (SSPD parameters are studied. We obtain an average recognition accuracy of 90% using SSPD for SVM based Malayalam CV speech unit database in speaker independent environments. The result shows that the efficiency of the proposed technique is capable for increasing speaker independent consonant speech recognition accuracy and can be effectively used for developing a complete speech recognition system for Malayalam language.

  8. Automatic segmentation of Leishmania parasite in microscopic images using a modified CV level set method

    Science.gov (United States)

    Farahi, Maria; Rabbani, Hossein; Talebi, Ardeshir; Sarrafzadeh, Omid; Ensafi, Shahab

    2015-12-01

    Visceral Leishmaniasis is a parasitic disease that affects liver, spleen and bone marrow. According to World Health Organization report, definitive diagnosis is possible just by direct observation of the Leishman body in the microscopic image taken from bone marrow samples. We utilize morphological and CV level set method to segment Leishman bodies in digital color microscopic images captured from bone marrow samples. Linear contrast stretching method is used for image enhancement and morphological method is applied to determine the parasite regions and wipe up unwanted objects. Modified global and local CV level set methods are proposed for segmentation and a shape based stopping factor is used to hasten the algorithm. Manual segmentation is considered as ground truth to evaluate the proposed method. This method is tested on 28 samples and achieved 10.90% mean of segmentation error for global model and 9.76% for local model.

  9. Programación de Aplicaciones OpenCV sobre Sistemas Heterogéneos SoC-FPGA

    OpenAIRE

    Sanchis Cases, Francisco José

    2014-01-01

    OpenCV es una biblioteca de primitivas de procesado de imagen que permite crear algoritmos de Visión por Computador de última generación. OpenCV fue desarrollado originalmente por Intel en 1999 para mostrar la capacidad de procesamiento de los micros de Intel, por lo que la mayoría de la biblioteca está optimizada para correr en estos micros, incluyendo las extensiones MMX y SSE. http://en.wikipedia.org/wiki/OpenCV. Actualmente es ampliamente utilizada tanto por la comunidad científica como p...

  10. Analisis Dan Rekayasa Supplier Relationship Management System Pada CV. Softcom Medan

    OpenAIRE

    Sukiman

    2012-01-01

    CV. SOFTCOM is a business entity engages in the computer sales and repair services. At the beginning of its establishment, the company did not see the importance of specialized brand in selling its product. The background of this study was the phenomenon in which many suppliers and different brands of the products found in the market that the company found it difficult to serve its customers claiming the damage of the sold products, the time of product delivered by the suppliers was frequentl...

  11. NIPPLE RECONSTRUCTION USING THE C-V FLAP TECHNIQUE: LONG-TERM OUTCOMES

    Directory of Open Access Journals (Sweden)

    Lona Jalini

    2016-08-01

    Full Text Available Introduction: Nipple creation using the C-V flap technique is often the final step in breast reconstruction. The aim of this study was to subjectively and objectively assess the cosmetic outcomes and satisfaction of patients undergoing C-V flap nipple reconstruction. Methods: Subjective assessments of patient satisfaction with the neo-nipple were recorded by visual analogue scoring (VAS; 0-10. Objective measurements were performed using a calliper to measure nipple projection relative to the native breast. Descriptive data analysis was performed with differences in projection assessed with the Mann-Whitney test and mean and median VAS scores (with inter-quartile ranges; IQR calculated to describe satisfaction. Results: Thirty-three C-V flap nipple reconstructions were performed. 87.9% received latissimus dorsi (LD reconstructions with implants and 12.1% had transverse rectus abdominis muscle (TRAM reconstructions. The median projection of reconstructed nipples was 4.7 mm (range 4-10.2 mm at 4.6 years mean follow-up, which was not significantly different from the contralateral nipple (p = 0.34. Patient satisfaction was 9 (IQR: 8-10 with shape, 9 (IQR: 7.5-10 with projection, 5 (IQR: 2-9.6 with sensation, and 8.5 (IQR: 6-9.5 with symmetry. Median overall satisfaction was 9 (IQR: 8-10. Three patients had complete nipple loss, of whom two had undergone nipple piercing post procedure and none had received radiotherapy. Conclusion: C-V flap nipple reconstructions provide a simple and reliable method to reconstruct the nipple that enhances confidence and perception of body image. Satisfaction was high with long-term outcomes in terms of projection equivalent to the contralateral breast.

  12. AT3 (Acyltransferase) Gene Isolated From Capsicum frutescens cv. Cakra Hijau

    OpenAIRE

    Mohamad Habibi; Andi Madhihah Manggabarani; Eko Sri Sulasmi; Dwi Listyorini

    2013-01-01

    Chili pepper is widely used and cultivated by Indonesian people. There are three species of chili pepper, i.e.: Capsicum annuum L., Capsicum frutescens L., and Capsicum violaceum HBK. Capsicum frutescens L. has a higher economic value due to its pungency and carotenoid content. C. frutescens has several cultivars, one of those is Capsicum frutescens cv. Cakra Hijau. This cultivar is resistant against pest and disease and has very high pungency. This special character of chili pepper is born b...

  13. Isolation of an immunosuppressive lectin from Phaseolus vulgaris L. cv Cacahuate using stroma.

    Science.gov (United States)

    Vargas-Albores, F; Hernández, J; Córdoba, F; Zenteno, E

    1993-11-01

    An immunosuppressive lectin was isolated from seed of Phaseolus vulgaris cv Cacahuate using physically entrapped stroma. The lectin was found to be a 94 kDa tetrameric protein. When 50 micrograms, of this lectin were administered intraperitoneally 2 days before the immunization with sheep red blood cells, humoral response against the immunogen was completely inhibited. Other properties of the protein are discussed. PMID:8248029

  14. A real-time camera calibration system based on OpenCV

    Science.gov (United States)

    Zhang, Hui; Wang, Hua; Guo, Huinan; Ren, Long; Zhou, Zuofeng

    2015-07-01

    Camera calibration is one of the essential steps in the computer vision research. This paper describes a real-time OpenCV based camera calibration system, and developed and implemented in the VS2008 environment. Experimental results prove that the system to achieve a simple and fast camera calibration, compared with MATLAB, higher precision and does not need manual intervention, and can be widely used in various computer vision system.

  15. Rancangan Sistem Kanban Pada Sistem Produksi Di Pabrik Kandang Baterai (Studi Kasus Di CV. Makmur Jaya)

    OpenAIRE

    Sofyan, Diana Khairani

    2011-01-01

    Industrial companies are currently making very rapid progresses, therefore, to keep surviving the existing competition, one of the ways they can do is to develop a more efficient and effective system of production such as the application of the Just in Time (JIT) principle precisely known as Kanban System. CV. Makmur Jaya, one of the chicken coop producing companies located in Binjai, is one of the companies that can apply the Kanban System. This company produces various products using kaw...

  16. Water use, transpiration, and crop coefficients for olives (cv. Cordovil), grown in orchards in Southern Portugal

    OpenAIRE

    Ramos, Alice; Santos, Francisco Lúcio

    2009-01-01

    To improve the scheduling of irrigation for low-density olive trees (Olea europaea L.) grown in a typical Mediterranean environment of Southern Portugal, and to clarify the mechanisms of water uptake by trees, transpiration, soil water status and stomatal response to water deficit were measured in an olive orchard. Olive trees of cv. Cordovil were subject to three irrigation treatments: full-rate irrigation, sustained deficit irrigation (SDI) providing for approximately 60% of water applied a...

  17. Diagnosis of Elevator Faults with LS-SVM Based on Optimization by K-CV

    OpenAIRE

    Zhou Wan; Shilin Yi; Kun Li; Ran Tao; Min Gou; Xinshi Li; Shu Guo

    2015-01-01

    Several common elevator malfunctions were diagnosed with a least square support vector machine (LS-SVM). After acquiring vibration signals of various elevator functions, their energy characteristics and time domain indicators were extracted by theoretically analyzing the optimal wavelet packet, in order to construct a feature vector of malfunctions for identifying causes of the malfunctions as input of LS-SVM. Meanwhile, parameters about LS-SVM were optimized by K-fold cross validation (K-CV)...

  18. Species of Pratylenchus Associated with Solanum tuberosum cv Superior in Ohio

    OpenAIRE

    Brown, M.J.; Riedel, R. M.; Rowe, R C

    1980-01-01

    Seventy-three Ohio fields comprising ca. 440 ha of cv Superior potatoes were surveyed in 1977 for plant-parasitic nematodes. Of eight genera of plant-parasitic nematodes, Pratylenchus was found most frequently, occurring in 65% of the soil samples and 84% of the root samples. Populations of Pratylenchus were consistently higher than populations of the other nematode genera. The six species of Pratylenchus extracted from potato roots, in descending order of frequency, were P. crenatus, P. pene...

  19. Comparison of direct mercury analyzer and FIA-CV-AAS in determination of methylmercury in fish

    Science.gov (United States)

    Ulrich, J. C.; Hortellani, M. A.; Sarkis, J. E. S.; Nakatsubo, M. A.

    2016-07-01

    Methylmercury (MeHg) has been determined in fish reference materials by direct mercury analyzer (DMA 80) and FIA-CV-AAS. In order to evaluate accuracy, certified reference materials (Fish protein, NRCC - Dorm 4 and fish material, Ipen - Dourada 1) were analyzed after extraction and separation of mercury species. Good agreement of the results have been obtained (relative error of the determination between the methods varied from 1.5% to 39%). The repeatability of the results varied from 4% to 26%.

  20. Frobenius type and CV-structures for Donaldson-Thomas theory and a convergence property

    CERN Document Server

    Barbieri, Anna

    2015-01-01

    We rephrase some well-known results in Donaldson-Thomas theory in terms of (formal families of) Frobenius type and CV-structures on a vector bundle in the sense of Hertling. We study these structures in an abstract setting, and prove a convergence result which is relevant to the case of triangulated categories. An application to physical field theory is also briefly discussed.

  1. Differences in Fruit Quality of Strawberry cv. Elsanta Depending on Cultivation System and Harvest Time

    OpenAIRE

    Sandra Voća; Nadica Dobričević; Martina Skendrović Babojelić; Jasmina Družić; Boris Duralija; Jasmina Levačić

    2007-01-01

    The aim of this research was to determine differences in chemical composition of strawberry cv. Elsanta fruit in three different cultivation systems and at three harvest times. Th e cultivation systems were: open field, high tunnel and hydroponics, all located in the Zagreb area. Th e harvest times were: the beginning, the middle and the end of strawberry harvest season. In the harvested fruit following parameters were determined: dry matter, total soluble solids (ºBrix), total acid (TA), Bri...

  2. Tinjauan Yuridis Terhadap Perjanjian Sewa Menyewa Mobil Di CV. Shandi Mocha Jaya Medan

    OpenAIRE

    Izhandri, Shandi

    2011-01-01

    Car leasing agreement resulted from the process of business development and this activity helps the community to run their own car rental business that can bring welfare to them. This car rental business in this study is a corporate body such as CV (Commanditaire Vennootschap = limited partnership) or PT (Perseroan Terbatas = Limited Liability Company) which is called service provider and the other party is called car hirer (the service user. The legal relationship between the two parties i...

  3. Strategi Pengembangan Bisnis Jasa Angkutan (Studi Pada CV. Batang Pane Baru, Jalan Sisingamangaraja Medan)

    OpenAIRE

    Hasibuan, Salman

    2015-01-01

    CV. Batang Pane Baru adalah salah satu usaha bisnis yang bergerak di bidang jasa transportasi bus di kota Medan. Dimana saat ini, banyak penduduk kota Medan yang membutuhkan penggunaan akan jasa transportasi bus tersebut. Dalam pengembangan usaha ini, dibutuhkan suatu startegi yang tepat agar pencapaian yang diinginkan oleh usaha tersebut dapat terkendali dengan baik dan benar sehingga kegiatan operasionalnya berjalan dengan lancar dalam memperluas pangsa pasar. Selain itu memudahkan usa...

  4. Anomaly in Cp/Cv: A possible signature of a liquid-glass transition

    International Nuclear Information System (INIS)

    Brillouin scattering from two classic glass-forming materials, ZnCl2 and glycerol, reveals a maximum in the ratio (γ) of specific heats Cp and Cv as a function of temperature. We propose that the temperature at which the maximum in γ occurs in our materials may be indicative of a ''liquid-like'' to ''solid-like'' transformation. As such it may be a convenient thermodynamic signature of a 'glass' transition

  5. CloudCV: Large Scale Distributed Computer Vision as a Cloud Service

    OpenAIRE

    Agrawal, Harsh; Mathialagan, Clint Solomon; Goyal, Yash; Chavali, Neelima; Banik, Prakriti; Mohapatra, Akrit; OSMAN, Ahmed; Batra, Dhruv

    2015-01-01

    We are witnessing a proliferation of massive visual data. Unfortunately scaling existing computer vision algorithms to large datasets leaves researchers repeatedly solving the same algorithmic, logistical, and infrastructural problems. Our goal is to democratize computer vision; one should not have to be a computer vision, big data and distributed computing expert to have access to state-of-the-art distributed computer vision algorithms. We present CloudCV, a comprehensive system to provide a...

  6. The Payment Vehicle Used in CV Studies of Environmental Goods Does Matter

    OpenAIRE

    Ivehammar, Pernilla

    2009-01-01

    This paper examines the effect of the payment vehicle on the valuation of an environmental good with the contingent valuation method (CVM). Results from three CV studies comparing different payment vehicles by using split samples when valuing environmental encroachment caused by roads in Sweden are presented and compared to results from other such split-sample studies of payment vehicle effects. The results are consistent and show that the payment vehicle affects the valuation, but not always...

  7. Development of seedlings of watermelon cv. Crimson Sweet irrigated with biosaline water

    OpenAIRE

    José E. S. B. da Silva; Janete R. Matias; Keylan S. Guirra; Carlos A. Aragão; Gherman G. L. de Araujo; Bárbara F. Dantas

    2015-01-01

    ABSTRACTThe limited access and the scarcity of good quality water for agriculture are some of the major problems faced in agricultural areas, particularly in arid and semiarid regions. The aim of this study was to evaluate the quality of watermelon seedlings (cv. Crimson Sweet), irrigated with different concentrations of biosaline water of fish culture. The experimental design was completely randomized with five treatments, corresponding to biosaline water at different concentrations (0, 33, ...

  8. Sward structure and livestock performance in guinea grass cv: Tanzania pastures managed by rotational stocking strategies

    OpenAIRE

    Valéria Pacheco Batista Euclides; Denise Bataglin Montagner; Gelson dos Santos Difante; Rodrigo Amorim Barbosa; Wellington Souza Fernandes

    2014-01-01

    Grazing strategy is a key element in the determination of sward structure, herbage nutritive value and animal performance. We aimed to compare the herbage characteristics and performance of livestock in pastures of Panicum maximum cv. Tanzania managed, using two rotational stocking strategies, which provided either a fixed-length rest period (FRP) of 35 days in the spring and fall and 30 days in the summer, or a variable-length rest period (VRP), determined by the time required for the canopy...

  9. Inflorescences production of heliconia cv. Golden Torch under nitrogen and potassium fertilization

    OpenAIRE

    Márkilla Zunete Beckmann-Cavalcante; Genilda Canuto Amaral; Rodrigo Cirqueira Avelino; Alcilane Arnaldo Silva; Alcimar de Sousa e Silva; João Batista da Silva Oliveira

    2015-01-01

    The heliconia plants are very demanding in nutrients, especially nitrogen and potassium, but there is a wide variation in their recommendation for fertilization. In this sense the objective was to evaluating the productive attributes of heliconia cv. Golden Torch in function of nitrogen and potassium fertilization in Bom Jesus-PI. The experiment was conductedin the experimental field of Floriculture in Campus Profa. “Cinobelina Elvas” (CPCE) at Federal University of Piauí (UFPI) in Bom Jes...

  10. 最权威的声音 Holden Monaro CV8-Z

    Institute of Scientific and Technical Information of China (English)

    李晓非

    2006-01-01

    让法拉利、保时捷、兰博基尼这些家伙都见鬼去吧,在澳大利亚,只有一款跑车可以大声说话,那就是霍顿Monaro。而作为最后一代的Monaro, Monaro CV8-Z依然在澳洲跑车界具有最权威的声音。

  11. Teaching image processing and pattern recognition with the Intel OpenCV library

    Science.gov (United States)

    Kozłowski, Adam; Królak, Aleksandra

    2009-06-01

    In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image processing and pattern recognition with the most up-to-date solutions available. In the Institute of Electronics at the Technical University of Lodz we facilitate the teaching process in this subject with the OpenCV library, which is an open-source set of classes, functions and procedures that can be used in programming efficient and innovative algorithms for various purposes. The topics of student projects completed with the help of the OpenCV library range from automatic correction of image quality parameters or creation of panoramic images from video to pedestrian tracking in surveillance camera video sequences or head-movement-based mouse cursor control for the motorically impaired.

  12. Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

    Directory of Open Access Journals (Sweden)

    Rong-Jyh Lin

    2014-02-01

    Full Text Available Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae, commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant, liriodenine (1, lysicamine (2, (--anonaine (3, (--asimilobine (4, (--caaverine (5, (--N-methylasimilobine (6, (--nuciferine (7, (--nornuciferine (8, (--roemerine (9, 7-hydroxydehydronuciferine (10 and cepharadione B (11 were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis. However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana.

  13. Efficient generation of CV entanglement by triply resonant non-degenerate OPA

    Science.gov (United States)

    Porzio, Alberto; D'Auria, Virginia; Fornaro, Stefano; Solimeno, Salvatore; Olivares, Stefano; Paris, Matteo G. A.

    2007-05-01

    CV entangled CW bright beams are experimentally generated by a Non-degenerate "triply resonant" Optical Parametric Amplifier (NOPA) based on PPKTP type-II crystal below threshold. Operating the OPA at frequency degeneracy makes the down-converted entangled beams able to be optically manipulated for generating different combination of the entangled system. Particular care has been required by the triply resonance condition essential to obtain a suffcient degree of entanglement. The triply resonance condition is pursued by combining temperature phase-matching and crystal tilting in an optical cavity so to optimize the triple resonance condition acting on different independent parameters. Exploiting the dependence of the bipartite system covariance matrix on different combination of this continuous variable two-mode states it can be directly measured using a single homodyne detector and few linear optical elements. The system is actually operating at the University of Napoli-Quantum Optics Laboratory. Preliminary measurements show that CV entanglement is present at the OPO output. This source of CV entanglement can be used for quantum communication purposes. The technical tricks herein implemented are interesting also for realizing triply resonant CW OPOs whose spectral properties and conversion efficiencies are better compared to single and double resonant devices. The quality of entanglement is also improved thanks to the use of a single cavity instead of dual cavities often employed in triply resonant devices. The measurement method can be applied to generic bipartite states made of frequency degenerate but orthogonally polarized modes.

  14. Production and composition of oranges cv. Lane Late under maintenance potassium fertilization

    Directory of Open Access Journals (Sweden)

    Gustavo Brunetto

    2016-04-01

    Full Text Available Este estudo teve como objetivo avaliar a produção e composição de laranjas de cv. Pista árvores tardias submetido a adubação de manutenção de potássio. O experimento foi conduzido em um pomar de cv. Lane Late em Rosário do Sul (RS. As laranjeiras foram submetidos a suplementação com 0, 50 ou 100% da dose recomendada de K2O em cada safra de 2008/2009 a 2011/2012. A produção de frutos, diâmetro do fruto, espessura da casca, volume de suco e suco, folha e os níveis de K no solo foram avaliados nas safras 2008/2009, 2010/2011 e 2011/2012. parâmetros de composição da fruta foram avaliados nos 2010/2011 e 2011/2012 safras. A aplicação de doses crescentes de potássio para CV. Pista laranjeiras atrasado para três safras não teve efeito sobre a produção de frutos. No entanto, a suplementação do solo com o potássio aumentou os níveis de nutrientes totais nas folhas e suco.

  15. Utrophins compensate for Dp71 absence in mdx3cv in adhered platelets.

    Science.gov (United States)

    Cerecedo, Doris; Mondragón, Ricardo; Candelario, Aurora; García-Sierra, Francisco; Mornet, Dominique; Rendón, Alvaro; Martínez-Rojas, Dalila

    2008-01-01

    Platelet adhesion is a critical step due to its hemostatic role in stopping bleeding after vascular damage. Short dystrophins are the most abundant dmd gene products in nonmuscle tissues, and in association with cytoskeleton proteins contribute to their intrinsic function; while utrophins are dystrophin-homologous related family proteins with structural and functional similarities. We previously demonstrated the presence of Dp71 isoforms, utrophins, and various dystrophin-associated proteins and their participation in cytoskeleton re-organization, filopodia and lamellipodia extension, and in centralizing cytoplasmic granules during the adhesion process of human platelets. To evaluate the morphologic changes and actin-based structures of mdx(3cv) platelets during the adhesion process, we compared the topographic distribution of Dp71d/Dp71Delta110(m) and dystrophin-associated protein in adhered platelets from dystrophic mdx(3cv) mouse. By confocal microscopy, we showed that absence of Dp71 isoforms in platelets from this animal model disrupted dystrophin-associated protein expression and distribution without modifying the platelet morphology displayed during the glass-adhesion process. By immunoprecipitation assays, we proved that up-regulated utrophins were associated with dystrophin-associated proteins to conform the dystrophin-associated protein complex corresponding to utrophins, which might compensate for Dp71 absence in mdx(3cv) platelets.

  16. AT3 (Acyltransferase Gene Isolated From Capsicum frutescens cv. Cakra Hijau

    Directory of Open Access Journals (Sweden)

    Mohamad Habibi

    2013-05-01

    Full Text Available Chili pepper is widely used and cultivated by Indonesian people. There are three species of chili pepper, i.e.: Capsicum annuum L., Capsicum frutescens L., and Capsicum violaceum HBK. Capsicum frutescens L. has a higher economic value due to its pungency and carotenoid content. C. frutescens has several cultivars, one of those is Capsicum frutescens cv. Cakra Hijau. This cultivar is resistant against pest and disease and has very high pungency. This special character of chili pepper is born by its secondary metabolic, Capsaicin. Moreover, capsaicin also serves as defense mechanism, antiarthritis, analgesic, and anticancer. This study aimed to isolate Acyltransferase (AT3 gene which encoding Capsaicin Synthase (CS enzyme. AT3 gene was isolated through PCR using forward primer 5’-ATG GCT TTT GCA TTA CCA TCA-3’ and reverse primer 5’-CCT TCA CAA TTA TTC GCC CA-3’. Data were analyzed using DNA Baser, BLAST, and ClustalX. This study has successfully isolated 404 bp fragments of AT3 gene. This fragments located at 1918-1434 bp referred to AT3 gene from Capsicum frutescens cv. Shuanla. Isolation of upstream and downstream fragments of AT3 gene from Capsicum frutescens cv. Cakra Hijau is undergoing.

  17. Physicochemical Composition, Phenolic Content and Antioxidant Activity of Sour Cherry cv. Marasca During Ripening

    Directory of Open Access Journals (Sweden)

    Sandra Pedisić

    2007-12-01

    Full Text Available Sour cherry cv. Marasca is Dalmatian cultivar from XVI century. Cultivation is limited on the north and central part of Dalmatia and on the part of the islands, where it achieves the best quality of fruit, high content of dry matter and sugar respectively, agreeable aroma and intense color. Sour cherry cv. Marasca is source of biologically active ingredients, organic and inorganic compounds, dietary fibers, aromatic compounds and high content of phenolic compounds, particularly anthocyanins and hormone melatonin. Many epidemiological studies showed that phenolic compounds have antioxidant and anti-inflammatory properties, and they have beneficial effect on human health. Marasca is rich with mentioned compounds. Physicochemical composition (total and soluble dry matter, pH value, total acidity during ripening of cv. Marasca ecotype Recta (Cerasus marasca recta grown in Zadar and Split area were determined. Total and soluble dry matter increased and pH value and total acidity showed little change during ripening. The content of phenolic compounds increased during ripening as well as content of anthocyanins, while antioxidant activity decreased with ripening. That indicates there was no correlation between antioxidant activity and content of total phenolic compounds.

  18. Aminoethoxivinilglicina no controle do amadurecimento de frutos de caqui cv. Fuyu Aminoethoxyvinylglycine in the ripening control of persimmon fruits cv. Fuyu

    Directory of Open Access Journals (Sweden)

    Angela Fuentes Fagundes

    2006-04-01

    Full Text Available Objetivou-se avaliar o efeito de aminoethoxivinilglicina (AVG, aplicado na pós-colheita, no amadurecimento de frutos de caqui (Diospyros kaki L. cv. Fuyu, armazenados à temperatura de 0 ± 2 ºC. Os frutos foram imersos durante dois minutos em solução de AVG, na concentração de 0; 415; 830 e 1.200 g ha-1 e dissolvidos em água destilada e adição de espalhante adesivo (óleo vegetal a 0,02% (v/v, secos à temperatura ambiente no barracão e armazenados em câmara fria a 0 ± 2 ºC e 95±2 % UR, e avaliados aos 32 e 52 dias com relação à firmeza, sólidos solúveis, pH da polpa, acidez titulável e injúria por frio. O AVG mostrou-se promissor no uso pós-colheita em caqui, onde os frutos tratados conservaram índices de firmeza linear em função da dose e teores de AT, SS e pH aceitáveis para o 'Fuyu'. Porém os frutos apresentaram translucidez, característica de injúria por frio, indicando a necessidade de novos estudos para se compreender a fisiologia pós-colheita deste fruto.An experiment was carried out to analyze the effect of the application of Aminoethoxyvinylglycine (AVG in post harvest application in persimmon fruits (Diospyros kaki L. cv. Fuyu stored at a temperature of 0 ± 2ºC. The fruits were immersed for two minutes into an AVG solution at a concentration of 0; 415; 830; 1200 g ha-1 and dissolved in distillated water. Then it was added adhesive spreader (vegetable oil 0.02% (v/v, dried at room temperature and stored at 0 ± 2 ºC and 95±2 % humidity. The treatments were evaluated on the 32nd and 52nd days to check the firmness, soluble solids, pH of the pulp, tritable acidity and chilling injury. The AVG may be promising in post harvest use with persimmon fruits, where treated fruits preserved linear levels of firmness in function of the doses and TA, SS and pH levels are acceptable for cv. Fuyu. However, fruits showed translucency, which are characteristics of chilling injury, indicating that new studies are

  19. EFEITO DO PERÍODO DE ARMAZENAMENTO SOBRE A BROTAÇÃO DE MUDAS DE BANANEIRA DO CULTIVAR NANICÃO (Musa acuminata AAA EFFECT OF STORAGE TIME IN THE BUDDING OF BANANA ROOTSTOCK CULTIVAR NANICÃO (Musa acuminata AAA

    Directory of Open Access Journals (Sweden)

    Ronaldo Veloso Naves

    2007-09-01

    Full Text Available

    A grande procura atual de mudas de bananeiras do cultivar Nanicão (Musa acuminata AAA em Goiás, principalmente na região do Mato Grosso Goiano, tem forçado o transporte de mudas a grandes distâncias. A maioria destas mudas são transportadas sob a forma de pedaços de rizoma com aproximadamente 1 Kg. Tem-se observado uma falha acima do previsto quando se utiliza este tipo de muda. O presente trabalho foi realizado para determinar o efeito do período de armazenamento sobre a brotação deste cultivar. Com base nos resultados concluiu-se que no período de armazenamento estudado (45 dias praticamente não houve diferença na brotação das mudas. Somente aquelas armazenadas por 5 (cinco semanas apresentaram inexplicavelmente um índice baixo de pegamento.

    Because of the tremendous current demand for Nanicão (Musa acuminata AAA banana shoots in the state of Goiás, notably in the “Mato Grosso Goiano” area, shoots have had to be imported from far away. The greater part of these shoots are transported in rootstalk form. Each rootstalk weighs approximately 1 Kg. A larger portion of these shoots than been anticipated, are defective. The present study was made to determine the effect of storage time on the budding of this particular banana rootstalk. The results of the experiment show that during the period of storage, forty-two (42 days, that was studied, there was almost no difference in budding among the rootstalks. Only those which were stored for five (5 weeks showed, unexplainably, a low budding index.

  20. A combined SEM, CV and EIS study of multi-layered porous ceramic reactors for flue gas purification

    DEFF Research Database (Denmark)

    He, Zeming; Andersen, Kjeld Bøhm; Nygaard, Frederik Berg;

    2013-01-01

    The effect of sintering temperature of 12-layered porous ceramic reactors (comprising 5 cells) was studied using scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The difference in microstructures of the reactors was evaluated by SEM...

  1. AAA Foundation for Traffic Safety

    Science.gov (United States)

    ... Wisconsin Goes into Effect Saturday - Patch.com California: self-driving cars will not need licensed driver, given federal approval - ... Guardian Smartphone use to be completely banned in cars from next year - Deccan Chronicle ... Will self-driving vehicles really make cities safer? - Curbed Hit a ...

  2. Indicadores funcionais de pastejo para o capim Brachiaria brizantha cv. Marandu (Functional indicators of grazing for the Brachiaria brizantha cv. Marandu grass

    Directory of Open Access Journals (Sweden)

    Bruno Ramalho Vieira

    2006-08-01

    Full Text Available O regime, ou padrão de desfolhação é a variável de maior influencia na resposta da planta ao pastejo. O padrão de desfolhação é definido pela intensidade (quantidade de material removido e frequência (número de vezes que a planta é desfolhada em um dado período de tempo de desfolhação. Esses indicadores funcionais de pastejo, juntamente com interceptação luminosa, altura, massa de forragem, adubação, taxa de lotação, subdivisões dos piquetes, etc... proporciona às plantas forrageiras um ambiente adequado para seu desenvolvimento, através da percepção de seus limites ecofisiológico, tendo como resultado a sustentabilidade do sistema. Para o capim Brachiaria brizantha cv. Marandu amplitude ótima de manejo em sistema de lotação contínua para produção de forragem variou de 20 a 40 cm de altura. The regime, or defoliation pattern is variable influences in the answer of the plant to the grazing. The defoliation pattern is defined by the intensity (amount of removed material and frequency (number of times that the plant is defoliated in a die period of time of defoliation. Those functional indicators of grazing, together with luminous interception, height, forage mass, manage, capacity rate, subdivisions of the pickets, etc... it provides to the plants an appropriate atmosphere for development, through the perception of their limits ecophysiological, tends as result the sustainability of the system. For the Brachiaria brizantha cv. Marandu grass great width of manage in system of continuous capacity for forage production varied from 20 to 40 cm of height.

  3. The effect of electroacupuncture on depressive rats's behavior induced by intracerebroventricular injection of L-AAA%电针对侧脑室注射L-AAA致抑郁模型大鼠行为学的影响

    Institute of Scientific and Technical Information of China (English)

    孙健; 姜硕; 刘健华; 符文彬; 蒋丽; 黄彬; 肖遥; 李富铭

    2013-01-01

    目的 观察侧脑室注射L-α-aminoadipic acid(L-AAA)对大鼠抑郁样行为的改变及电针的治疗效应.方法 将45只雌性SD大鼠随机分为空白组、侧脑室注射生理盐水组(侧注NS组)、侧脑室注射L-AAA组(侧注LAAA组)、针刺组、利鲁唑灌胃组(药物组),每组9只;观察侧脑室注射L-AAA后大鼠行为学的变化及电针、药物对侧脑室注射L-AAA模型大鼠抑郁样行为的改善作用.结果 侧脑室注射L-AAA后大鼠出现体重下降、新环境抑制饮食时间延长、糖水消耗率下降和运动量减少等类抑郁症状,与空白组及侧注NS组比较具有显著性差异(P<0.01);经电针、药物治疗后大鼠的类抑郁症状明显减轻,与侧注射LAAA组比较具有显著性差异(P<0.01),基本恢复至正常水平(空白组、侧注NS组).结论 侧脑室注射LAAA后可以诱发大鼠的类抑郁症状,而电针可以明显改善此类症状,且疗效与利鲁唑相似.据此推测电针抗抑郁的机制可能与促进星形胶质细胞修复有关.%Objective To observe the effect of intracerebroventricular injection of L- α -aminoadipic acid ( L-AAA) inducing to depressive behavior in rats and the effect of electroacupuncture ( EA) on it. Methods 45 female SD rats were randomly distributed into blank, intracerebroventricular injection of normal saline ( NS) , intracerebroventricular injection of L-AAA ( LAAA) , acupuncture, riluzole gavage ( drug) groups, 9 rats for each. The behavior changes of rats after intracerebroventricular infusion of L-AAA and the effect of electroacupuncture , drugs on these depression-like behavior of intracerebroventricular injection of L-AAA model rats were observed. Results ( 1 ) There were depressive symptoms in rats after intracerebroventricular injection of L-AAA, including weight loss, new environment restrain food time prolonged, sucrose consumption rate decreasing and exercise loss, the difference was statistically significant compared

  4. 5个香蕉穿孔线虫种群对香蕉的致病性测定%Determination of Pathogenicity of 5 Populations of Radopholus similis to 3 Banana Cultivars

    Institute of Scientific and Technical Information of China (English)

    符美英; 吴凤芝; 卜小莉; 王会芳; 陈绵才

    2011-01-01

    Radopholus similis is the most serious pest of banana. The pathogenicity of 5 populations of R. Similis to seedlings of 3 banana cultivars was tested by pot-inoculation in greenhouse. The results showed that all of the 5 populations of R. Similis were highly pathogenic to Musa AAA Giant Cavendish cv. Baxi. RSI population of R. Similis showed weak pathogenicity to Musa ABB Pisang Awak and the other four populations showed no pathogenicity to Musa ABB Pisang Awak. Musa acuminata cv. Mas was not the host of these 5 R. Similis populations. Musa AAA Giant Cavendish cv. Baxi plants parasitized with R. Similis showed a reduced growth with yellowing leaves and browning spot in roots.%香蕉穿孔线虫对香蕉生产是一种毁灭性的危害.为了明确5个香蕉穿孔线虫种群对香蕉的致病性,在温室中采用盆栽接种的方法,分别测定了5个香蕉穿孔线虫花卉种群对巴西蕉、皇帝蕉和粉蕉的寄生性.结果表明,5个香蕉穿孔线虫种群对巴西蕉都有强致病性;RS1种群对粉蕉存在弱致病性,其他4个种群对粉蕉均没有致病性;皇帝蕉不是这5个香蕉穿孔线虫种群的寄主植物.香蕉穿孔线虫寄生巴西蕉后,造成香蕉植株明显矮小、长势弱,严重时地上部叶片出现萎蔫黄化,根部形成褐色病斑甚至坏死变黑腐烂等症状,对巴西蕉危害显著.

  5. Analisis Pengaruh Lokasi, Produk, dan Pelayanan Pada Pelanggan Terhadap Loyalitas Konsumen di Percetakan CV. Waty Grafika Medan

    OpenAIRE

    Aisyah,, Nurnaning

    2011-01-01

    Printing company in Medan is growing rapidly. In this town, there are many printing companies in considerable numbers. This results an increasing competition in the field. CV. Waty Grafika Medan, which is located at Jalan Jamin Ginting 140 Medan, has established since January 1984. The problem in this research is to examine how the location, product, and customer service of CV. Waty Grafika Medan affect on its customer satisfaction and how it then affects on the customer loyalty at this compa...

  6. Defective secretion of mucilage is the cellular basis for agravitropism in primary roots of Zea mays cv. Ageotropic

    Science.gov (United States)

    Miller, I.; Moore, R.

    1990-01-01

    Root caps of primary, secondary, and seminal roots of Z. mays cv. Kys secrete large amounts of mucilage and are in close contact with the root all along the root apex. These roots are strongly graviresponsive. Secondary and seminal roots of Z. mays cv. Ageotropic are also strongly graviresponsive. Similarly, their caps secrete mucilage and closely appress the root all along the root apex. However, primary roots of Z. mays cv. Ageotropic are non-responsive to gravity. Their caps secrete negligible amounts of mucilage and contact the root only at the extreme apex of the root along the calyptrogen. These roots become graviresponsive when their tips are coated with mucilage or mucilage-like materials. Peripheral cells of root caps of roots of Z. mays cv. Kys contain many dictyosomes associated with vesicles that migrate to and fuse with the plasmalemma. Root-cap cells of secondary and seminal (i.e. graviresponsive) roots of Z. mays cv. Ageotropic are similar to those of primary roots of Z. mays cv. Kys. However, root-cap cells of primary (i.e. non-graviresponsive) roots of Z. mays cv. Ageotropic have distended dictyosomal cisternae filled with an electron-dense, granular material. Large vesicles full of this material populate the cells and apparently do not fuse with the plasmalemma. Taken together, these results suggest that non-graviresponsiveness of primary roots of Z. mays cv. Ageotropic results from the lack of apoplastic continuity between the root and the periphery of the root cap. This is a result of negligible secretion of mucilage by cells along the edge of the root cap which, in turn, appears to be due to the malfunctioning of dictyosomes in these cells.

  7. 基于AAA服务器策略的企业网动态安全访问的实现%Implementation of Dynamic Security Access of Entranet Based on AAA Server Policy

    Institute of Scientific and Technical Information of China (English)

    彭亚发; 洪晓峰

    2013-01-01

    the AAA server strategy can achieve the function of IEEE802.1X includes authorization, authentication and statistics. In this paper, through the application of AAA server in enterprise network to solve different internal server to meet different user access requirements. Through the AAA server strategy not only can effectively control the unauthorized user access to internal corporate servers, pose a threat to the enterprise information security, but also can avoid the network device configuration is very complex ACL access rules cause equipment burden reduced performance of the phenomenon, to improve the efficiency of enterprise network and realize the dynamic security of network users enterprise network access.%利用AAA服务器策略可以实现IEEE802.1X的授权、认证和统计功能,通过在企业网络中应用AAA服务器策略解决企业内部不同的服务器满足不同用户访问的需求,通过AAA服务器策略不但能够有效控制未经授权的用户访问企业内部服务器,对企业信息安全造成威胁,而且能够避免在网络设备上配置相当复杂的ACL访问规则造成设备负担过重而性能降低的现象,从而提高了企业网络的运行效率并实现网络用户对企业网络的动态安全访问。

  8. Two AAA Family Peroxins, PpPex1p and PpPex6p, Interact with Each Other in an ATP-Dependent Manner and Are Associated with Different Subcellular Membranous Structures Distinct from Peroxisomes

    OpenAIRE

    Faber, Klaas Nico; Heyman, John A.; Subramani, Suresh

    1998-01-01

    Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris. Cells from the corresponding deletion strains (PpΔpex1 and PpΔpex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the cytosol, and these cells cannot grow in peroxisome-requiring media (J. A. Heyman, E. Monosov, and S. Subramani, J. Cell Biol. 127:1259–1273, 1994; A. P. Spong and S. Subramani, J. Cell Biol....

  9. The Logos of A!A"A? of The Aalto University School of Art and Design%从A!A”A?三款校徽标识所悟到的

    Institute of Scientific and Technical Information of China (English)

    黄迅

    2012-01-01

    去年暑假期间。我们一行十几人,在广东工业大学艺术设计学院院长方海教授的带领下,开始了为期十二天对芬兰阿尔托大学的考察学习。%The paper records the writer's visit to The Aalto University School of Art and Design in Finland, especially how the writer got confused about the Iogos of A!A"A? of The Aalto University School of Art and Design at first and finally figured it out.

  10. Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. 'Liscia' and C. medica cv. 'Rugosa' Cultivated in Southern Italy.

    Science.gov (United States)

    Aliberti, Luigi; Caputo, Lucia; De Feo, Vincenzo; De Martino, Laura; Nazzaro, Filomena; Souza, Lucéia Fátima

    2016-01-01

    Citrus medica cv. 'liscia' and C. medica cv. 'rugosa' are two taxa of citron, belonging to the biodiversity of South Italy, in particular of Amalfi Coast, in the Campania region. The chemical composition of the essential oils (EOs) from fruit peels of both C. medica cultivars was studied by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses. In all, 100 compounds were identified, 82 for C. medica cv. 'liscia', accounting for 91.4% of the total oil, and 88 for C. medica cv. 'rugosa', accounting for 92.0% of the total oil. Monoterpene hydrocarbons are the main constituents in both oils of C. medica cv. 'liscia' (79.1%) and C. medica cv. 'rugosa' (80.2%). In both oils, limonene (67.2%-62.8%) and camphene (8.5%-10.9%) are the main constituents. The antimicrobial activity of the EOs was assayed against some bacterial strains: Bacillus cereus (DSM 4313), Bacillus cereus (DSM 4384), Staphylococcus aureus (DSM 25693), Pseudomonas aeruginosa (ATCC 50071), and Escherichia coli (DSM 8579). Low concentrations of C. medica cv. 'rugosa' EO showed an inhibitory effect on P. aeruginosa and higher concentrations inhibited more B. cereus (4384) and E. coli than S. aureus. The cytotoxicity of the EO was evaluated against SH-SY5Y cell line. The influence of the EO on the expression of adenylate cyclase 1 (ADCY1) was also studied. The antimicrobial activity registered confirm their traditional uses as food preserving agents and led us to hypothesize the possible use of these oils as antimicrobials. The alterations in ADCY1 expression suggested a role for limonene in effects on the central nervous system. PMID:27649138

  11. Pulmonary nodule detection in CT images based on shape constraint CV model

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Bing; Tian, Xuedong [College of Mathematics and Computer Science, Hebei University, Baoding 071002 (China); Wang, Qian [Hebei Geological Laboratory, Baoding 071000, China and Multi-disciplinary Research Center, Hebei University, Baoding 071002 (China); Yang, Ying [Hebei University Affiliated Hospital, Baoding 071002 (China); Xie, Hongzhi, E-mail: gulixu@sjtu.edu.cn, E-mail: xiehongzhi@medmail.com.cn; Zhang, Shuyang [Department of Cardiology, Peking Union Medical College Hospital, Peking 100005 (China); Gu, Lixu, E-mail: gulixu@sjtu.edu.cn, E-mail: xiehongzhi@medmail.com.cn [Multi-disciplinary Research Center, Hebei University, Baoding 071002, China and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030 (China)

    2015-03-15

    Purpose: Accurate detection of pulmonary nodules remains a technical challenge in computer-aided diagnosis systems because some nodules may adhere to the blood vessels or the lung wall, which have low contrast compared to the surrounding tissues. In this paper, the analysis of typical shape features of candidate nodules based on a shape constraint Chan–Vese (CV) model combined with calculation of the number of blood branches adhered to nodule candidates is proposed to reduce false positive (FP) nodules from candidate nodules. Methods: The proposed scheme consists of three major stages: (1) Segmentation of lung parenchyma from computed tomography images. (2) Extraction of candidate nodules. (3) Reduction of FP nodules. A gray level enhancement combined with a spherical shape enhancement filter is introduced to extract the candidate nodules and their sphere-like contour regions. FPs are removed by analysis of the typical shape features of nodule candidates based on the CV model using spherical constraint and by investigating the number of blood branches adhered to the candidate nodules. The constrained shapes of CV model are automatically achieved from the extracted candidate nodules. Results: The detection performance was evaluated on 127 nodules of 103 cases including three types of challenging nodules, which are juxta-pleural nodules, juxta-vascular nodules, and ground glass opacity nodules. The free-receiver operating characteristic (FROC) curve shows that the proposed method is able to detect 88% of all the nodules in the data set with 4 FPs per case. Conclusions: Evaluation shows that the authors’ method is feasible and effective for detection of three types of nodules in this study.

  12. IMPLEMENTASI SIX SIGMA UNTUK PENINGKATAN KUALITAS SANDAL DI CV. SANCU CREATIVE INDONESIA

    Directory of Open Access Journals (Sweden)

    Sonny Koeswara

    2013-10-01

    Full Text Available Six Sigma adalah metodologi yang terstruktur untuk memperbaiki proses yang difokuskan pada usaha mengurangi variasi pada proses sekaligus mengurangi defect pada produk dengan menggunakan pendekatan statistik dan Problem solving tools secara intensif. Tujuan dari metode Six Sigma ini adalah untuk mendapatkan zero defect dengan menjalankan 5 tahapan yaitu : Define, Measure, Analyze, Improve ,dan Control (DMAIC. Tulisan ini bertujuan untuk menerapkan metode Six Sigma di Cv. Sancu Creative Indonesia yang merupakan badan usaha yang memproduksi sandal karakter. Masalah yang terjadi adalah adanya defect sol sandal mudah mengelupas yang berakibat pada kualitas sandal dan salah satu penghambat visi dan misi perusahaan. Penelitian ini dibagi dalam 5 tahap. Tahap pertama adalah Define, yang bertujuan untuk mengidentifikasi masalah yang terjadi yang berasal dari kebutuhan konsumen berdasarkan data DCA dan OQC defect di CV. SANCU. Tahap kedua adalah Measure yaitu mengukur kondisi level performance proses saat ini (Z-Level. Pada tahap ini dilakukan validasi sistem pengukuran  dengan  menghitung  Dpo,Dpmo,Yield  dan  Sigma  level.  Tahap  ketiga  adalah Analyze yaitu menentukan faktor-faktor penyebab terjadinya sol sandal mengelupas dan sekaligus menentukan vital faktor. Hasil dari pengujian menunjukkan bahwa vital faktor adalah waktu penempelan.Tahap keempat adalah improvement yaitu melakukan perbaikan dengan memberikan timer diarea lokasi penempelan. Control yaitu kegiatan melakukan pemantauan dari hasil perbaikan yang telah di implementasikan Kondisi   setelah   perbaikan   menghasilkan   perubahan   yang   signifikan   terhadap kapabilitas performance pada sandal, yaitu perubahan nilai sigma level setelah perbaikan 4.74s dari yang sebelum perbaikan adalah 4.26 s. Dari nilai tersebut dapat disimpulkan bahwa metode waktu penempelan dan kontrol pada performance sandal Cv. Sancu sudah membaik.

  13. Transformation of indica rice (Oryza sativa L. cv. RD6 mediated by Agrobacterium tumefaciens

    Directory of Open Access Journals (Sweden)

    Manit Kosittrakul

    2004-01-01

    Full Text Available High percentage of callus induction at 97% was obtained when seeds of rice (Oryza sativa L. cv. RD6 were cultured on modified N6 medium supplemented with 3% (w/v sucrose, 22.5 μM 2,4-D and 0.8% agar under light condition. The suitable regeneration medium was N6 medium supplemented with 3% (w/v sucrose, 2.5 μM IAA, 18 μM BA and 0.8% agar. A test had been performed to determine the effect of antibiotics on the regeneration of rice cv. RD6. It was found that kanamycin concentration up to 150 mg l-1 and hygromycin concentration at 10 mg l-1 were effective for selection of transformants. Cefotaxime and carbenicillin concentration up to 250 mg l-1 had the highest phytotoxicity to plant regeneration. Agrobacterium-mediated gene transfer protocols for rice cv. RD6 were performed using A. tumefaciens strain LBA4404, which harbored the plasmid pBI121 containing genes for β- glucuronidase (GUS and kanamycin resistance (nptII, and strain EHA105, which harbored plasmid pCAMBIA1301 containing genes for β-glucuronidase (GUS and hygromycin resistance (hptII. GUS activities were found in rice calli after co-cultivation. A number of morphologically normal fertile transgenic rice plants were obtained. Stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transgenic plants in T0 and T1 generation. Mendelian segregation was observed in T1 progeny.

  14. TREATMENT OF 80 CASES OF INFANTILE DIARRHEA WITH ABDOMINAL ACUPUNCTURE AND MOXIBUSTION ON SHENQUE (CV 8)

    Institute of Scientific and Technical Information of China (English)

    PU Shang-xi; CHEN Pu-qing

    2006-01-01

    @@ Infantile diarrhea is the common and frequently-occurred disease in the pediatric department, fallen into the cate gory of "Xiaoer Xiexie" (infantile diarrhea) in Chinese medicine, which is commonly caused by the invasion of exogenous pathogenic factors, improper diet, the yang deficiency of the spleen and kidney and the weakness of the spleen and stomach. Eighty cases of infantile diarrhea had been treated with the abdominal acupuncture and moxibustion on Shenque (神阙CV 8) and the satisfactory results had been achieved. The report is as follows.

  15. Faktor-Faktor Yang Berhubungan Dengan Kelelahan Pengemudi Bus di CV. Makmur Medan Tahun 2014

    OpenAIRE

    Syahlefi, Meutia Reza

    2016-01-01

    This research was conducted on driver 1 of CV. Makmur heading for Pekan Baru from Medan (round trip) in 2014 in order to find out some factors which were correlated with the incidence of fatigue. The research was an analytic survey with cross sectional design which was aimed to find out the correlation of the factors of age, driving duration, physical condition, break time, and nutrition/IMT status with the incidence of fatigue. The population was 160 drivers, and 32 of them were used as t...

  16. Development of high yielding mutants of Brassica campestris L. cv. Toria selection through gamma rays irradiation

    International Nuclear Information System (INIS)

    Homogeneous seeds of Brassica campestris L. cv. Toria selection were treated with different doses of gamma rays (750, 1000 and 1250 Gy) to induce genetic variability for the selection of new genotypes with improved agronomic traits. After passing through different stages of selection, two promising mutants were selected for further studies. Two selected mutants along with 5 other entries including parent variety were evaluated for yield and yield components in yield trials for two consecutive years. The mutant TS96-752 was significantly (P less than or equal to 0.05) superior to all other entries in grain yield but at par with FSD 86028-3

  17. Plant regeneration in vitro of South Pacific taro (Colocasia esculenta var. esculenta cv. Akalomamale, Aracea).

    Science.gov (United States)

    Yam, T W; Hsu, G I; Arditti, J

    1990-08-01

    Axillary bud expiants from South Pacific (Solomon Islands) taro, Colocasia esculenta var. esculenta cv. Akalomamale (Araceae) cultured on a modified Murashige-Skoog medium containing 1 mg NAA 1(-1) and TE formed callus and produced multiple plantlets. Explants died if NAA was present at levels lower than 0.1 mg 1(-1). BA was not required and may have been inhibitory. Plantlets developed faster and became larger following transfer to a hormone-free medium two weeks after the start of culture. Fully grown plants were established in a potting mix and are growing well in a greenhouse.

  18. In Vivo and in Vitro Micrografting of Pistachio, Pistacia vera L. cv. "Siirt"

    OpenAIRE

    ONAY, Ahmet; PİRİNÇ, Vedat; ADIYAMAN, Filiz; IŞIKALAN, Çiğdem; TİLKAT, Engin; BAŞARAN, Davut

    2003-01-01

    In this study, the success of in vivo and in vitro micrografts of pistachio (Pistacia vera L. cv. "Siirt") materials are presented. The only variable tested was age (1, 5, 10, and 30-year-old trees). Ten- to 12-day-old axenic seedlings germinated in vitro or seedlings (3 to 5 months-old) grown in pots in vivo were used as rootstocks. Shoot tips collected from the four age classes of mature trees of pistachio were the source of scions. Firm contact between the scion and rootstock was...

  19. Induction of chlorophyll chimeras and chlorophyll mutations in mungbean (Vigna radiata) cv. T44

    International Nuclear Information System (INIS)

    Uniform and healthy seeds of mungbean (Vigna radiata) cv. T44 were exposed to varying doses of gamma rays, ethyl methane sulphonate (EMS) and combination treatment of gamma rays with EMS. The data were recorded for seed germination, plant survival, frequency and spectrum of chlorophyll chimeras in M1 and chlorophyll mutations in M2 generation. Among all, the combination treatments were found most effective for inducing chlorophyll chimeras and chlorophyll mutations than the gamma rays or EMS alone. Of the mutants under reference, the albino, xantha and chlorina showed monogenic recessive while viridis exhibited digenic recessive inheritance. (author). 8 refs., 2 tabs

  20. RECUPERAÇÃO DE SALMONELLA SPP. EM MANGA (MANGIFERA INDICA) CV. TOMMY ATKINS

    OpenAIRE

    Silvana Belém de Oliveira Vilar; Maria Fernanda Pontes Penteado Moretzhon de Castro; Ana Carolina Rezende; Ana Lúcia Penteado; Flávio Luís Schmidt

    2015-01-01

    Salmonella spp. é um patógeno que se destaca como agente responsável por doenças veiculadas por alimentos. Alguns estudos demonstram que o consumo de manga contaminada foi responsável por alguns destes surtos. Este trabalho objetivou investigar a eficiência de diferentes metodologias para a recuperação de Salmonella em manga (Mangifera indica) cv. Tommy Atkins. Para o estudo, as mangas foram artificialmente contaminadas com um pool de Salmonella contendo culturas de Salmonella Typ...

  1. VARYING DEGREE OF GRAFTING COMPATIBILITY BETWEEN CV. CHARDONNAY, MERLOT AND DIFFERENT GRAPEVINE ROOTSTOCKS

    Directory of Open Access Journals (Sweden)

    Slavica TODIĆ

    2006-02-01

    Full Text Available Level of affi nity between grapevine rootstock and Vitis vinifera as scion, quality of reproductive materials and technological actions in grapevine rootstock production process determine success in grapevine rootstock production in large extent. Practical training showed that difference in level of compatibility between grapevine rootstock and grafted Vitis vinifera cultivars are existing. Direct effects of these differences are unequal yield of fi rst class grafted grapevine rootlings. In this paper, level of compatibility in nursery between clones of cv. Chardonnay BCL 75, VCR4 and cv. Merlot R18, MCL 519 and grapevine rootstocks Kober 5BB (Vitis berlandieri x V. riparia, SO4 (V. berlandieri x V. riparia and 41B (Chasselas x V.berlandieri were investigated. The trial was conducted in commercial grapevine nursery located in Velika Drenova, Serbia. As an index of compatibility, grade of high quality grapevine grafted rootlings, dry matter in mature shoots and root system development were used. Grafting was done by `tongue grafting` indoor technique. Stratifi cation was done in sand, on temperature of the stratifi cation material of 26-28oC, and humidity of around 90%. Grafted cuttings were waxed twice: before stratifi cation, and before planting in the nursery. Grafted rootlings were classed in two classes according to regulations of quality, (Yugoslav Offi cial Register, 26/79. Grafted rootlings that did not satisfi ed standard criteria were discarded. Both clones of cv. Chardonnay gave the highest percentage of I class grafted rootlings on grapevine rootstock 41B: clone BCL 75 – 60% and clone VCR4 – 61%. In the same combination, those grapevine grafted rootlings had the highest weight of the root system. Lower percentage of obtained I class grafted rootlings was established on rootstock Kober 5BB, while statistically signifi cantly lower yields were obtained on grapevine rootstock SO4: clone BCL75 – 43% and clone VCR4 – 48%. Dry

  2. Some features of one-year-old tart Cherry shoots of cv. Sehattenmorelle

    OpenAIRE

    Dariusz Świetlik; Kazimierz Słowik; Sebastian Rejman

    2013-01-01

    The purpose of this study was to obtain data on the relationship between the length of 1-year-old shoots and their fruiting, and both their terminal and lateral growtn produced in the current year. The measurements were taken in 1977 and 1979 from the tart cherry trees of cv. Schattenmorelle grafted of Prunus mahaleb L. or Prunus avium L. grown in loose sandy soil. In 1977 there were more shoots of over 30 cm than in 1979. The longer the shoots, the more lateral shoots were there. Only the sh...

  3. Some features of one-year-old tart Cherry shoots of cv. Sehattenmorelle

    Directory of Open Access Journals (Sweden)

    Dariusz Świetlik

    2013-12-01

    Full Text Available The purpose of this study was to obtain data on the relationship between the length of 1-year-old shoots and their fruiting, and both their terminal and lateral growtn produced in the current year. The measurements were taken in 1977 and 1979 from the tart cherry trees of cv. Schattenmorelle grafted of Prunus mahaleb L. or Prunus avium L. grown in loose sandy soil. In 1977 there were more shoots of over 30 cm than in 1979. The longer the shoots, the more lateral shoots were there. Only the shortest shoots (1-10 cm bore more fruit per unit length as compared to the longer shoots.

  4. Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

    OpenAIRE

    Glaucia Almeida de Morais; Massanori Takaki

    1998-01-01

    Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used w...

  5. Three in one go: consequential angular momentum loss can solve major problems of CV evolution

    CERN Document Server

    Schreiber, Matthias R; Wijnen, Thomas P G

    2015-01-01

    The average white dwarf (WD) masses in cataclysmic variables (CVs) have been measured to significantly exceed those of single WDs, which is the opposite of what is theoretically expected. We present the results of binary population synthesis models taking into account consequential angular momentum loss (CAML) that is assumed to increase with decreasing WD mass. This approach can not only solve the WD mass problem, but also brings in agreement theoretical predictions and observations of the orbital period distribution and the space density of CVs. We speculate that frictional angular momentum loss following nova eruptions might cause such CAML and could thus be the missing ingredient of CV evolution.

  6. Rendimiento y componentes del valor nutritivo del Panicum maximum cv. Tanzania

    OpenAIRE

    Verdecia, Danis M.; Ramírez, Jorge L.; Leonard, Ismael; Pascual, Yoandris; López, Yoel:

    2008-01-01

    El trabajo fue realizado con el objetivo de determinar el rendimiento y algunos componentes del valor nutritivo del valor nutritivo del Panicum maximum cv. Tanzania a diferentes edades de rebrote, para esto se midió una parcela de 1600 m2 , aplicando un corte de uniformidad a 10 cm de altura del suelo. Las edades evaluadas fueron los 30, 45, 60, 75, 90 y 105 días. Se evaluaron el rendimiento, la composición química, la digestibilidad y energía metabolizable. Se empleó un diseño en bloques al ...

  7. Nitrogen and potassium fertilization in yield and macronutrients contents of heliconia cv. Golden Torch

    OpenAIRE

    Márkilla Z. Beckmann-Cavalcante; Genilda C. Amaral; Alcimar de S. e Silva; Leonardo P. da S. Brito; Augusto M. N. Lima; Ítalo H. L. Cavalcante

    2016-01-01

    ABSTRACT This study aimed to evaluate the effect of nitrogen (N) and potassium (K) fertilization in yield and contents of macronutrients in heliconia plants (Heliconia psittacorum x Heliconia spathocircinata Aristeguieta) cv. Golden Torch. The experiment was set in a randomized block design, in a 4 x 4 factorial, corresponding to N doses (0, 120, 180 and 240 g of N hole-1) and K doses (0, 120, 180 and 240 g of K2O hole-1) with four replicates and five rhizomes per plot, under field conditions...

  8. Enraizamento e aclimatização de plantas micropropagadas de amoreira-preta cv. Brazos Rooting and acclimatization of micropropagated plants of blackberry cv. Brazos

    Directory of Open Access Journals (Sweden)

    Carolina Smanhotto Schuchovski Augusto

    2006-12-01

    Full Text Available O presente trabalho estudou as fases finais do processo de micropropagação, incluindo o enraizamento e a posterior aclimatização da amoreira-preta cv. Brazos. Os experimentos foram realizados no Laboratório de Micropropagação de Plantas da UFPR, Curitiba-PR, no período de março de 2000 a julho de 2001. No enraizamento in vitro com ou sem imersão em AIB, obteve-se mais de 95% de enraizamento nos dois tratamentos. No experimento de enraizamento ex vitro, com microestacas provenientes da multiplicação com diferentes citocininas, as taxas de enraizamento e sobrevivência foram de 100%. Na fase final, testou-se a influência da sacarose do meio de cultura de enraizamento in vitro na posterior aclimatização. Em todos os tratamentos, houve 100% de sobrevivência. Conclui-se que pode ser realizado um eficiente enraizamento in vitro sem AIB e sem a adição de sacarose ao meio de cultura, com posterior aclimatização em túnel plástico ou, ainda, realizar o enraizamento ex vitro e aclimatização em casa de vegetação com nebulização intermitente.The present work studied the final stages of blackberry cv. Brazos micropropagation, including rooting and acclimatization. The experiments were carried out in the Plant Micropropagation Laboratory at UFPR, Curitiba-PR, during March 2000 until July 2001. In the in vitro rooting with or without immersion in IBA, the percentage of rooting was higher than 95% in both treatments. In the experiment of ex vitro rooting, with micro-shoots proceeding from the multiplication with different cytokinins, the rooting and survival rates were of 100%. In the final stage it was tested the influence of sucrose of the in vitro rooting media on the acclimatization. All the treatments showed 100% of survival. It can be concluded that an efficient in vitro rooting can be realized without both IBA and sucrose in the culture media with acclimatization under plastic tunnel or ex vitro rooting and acclimatization

  9. Design and Implementation of Camera Calibration System Based on OpenCV and Matlab%基于 OpenCV 和 Matlab 的摄像机标定系统设计与实现

    Institute of Scientific and Technical Information of China (English)

    江祥奎; 纪旭

    2015-01-01

    Aiming at camera calibration technology ,the characteristics of OpenCV library and the function model of Matlab calibration toolbox are analyzed .The advantages are used to design a kind of camera calibration system .The system gives full play to the performance of OpenCV function library ,uses Matlab calibration kit as a supplement .Finally the sys-tem is implemented by GUI interface programming method .The system has high calibration precision ,friendly interface , strong expansibility .It can better satisfy the engineering need of machine vision system .%以摄像机标定技术为研究对象,对 OpenCV 函数库和 Matlab 标定工具箱中的函数模型进行分析,结合二者优势设计了一款摄像机标定系统。该系统充分发挥了 OpenCV 函数库的性能,并通过 Matlab 标定工具箱对 OpenCV 函数进行补充。最后通过 GUI 方法进行界面编程。实验结果表明,该系统具有较高的标定精度、界面友好、扩展性强,可以更好地满足机器视觉系统的工程需要。

  10. 基于 OpenCV 和 Matlab 的摄像机标定系统设计与实现%Design and Implementation of Camera Calibration System Based on OpenCV and Matlab

    Institute of Scientific and Technical Information of China (English)

    江祥奎; 纪旭

    2015-01-01

    以摄像机标定技术为研究对象,对 OpenCV 函数库和 Matlab 标定工具箱中的函数模型进行分析,结合二者优势设计了一款摄像机标定系统。该系统充分发挥了 OpenCV 函数库的性能,并通过 Matlab 标定工具箱对 OpenCV 函数进行补充。最后通过 GUI 方法进行界面编程。实验结果表明,该系统具有较高的标定精度、界面友好、扩展性强,可以更好地满足机器视觉系统的工程需要。%Aiming at camera calibration technology ,the characteristics of OpenCV library and the function model of Matlab calibration toolbox are analyzed .The advantages are used to design a kind of camera calibration system .The system gives full play to the performance of OpenCV function library ,uses Matlab calibration kit as a supplement .Finally the sys-tem is implemented by GUI interface programming method .The system has high calibration precision ,friendly interface , strong expansibility .It can better satisfy the engineering need of machine vision system .

  11. Crecimiento en clavel estándar cv. Nelson, en suelo y en sustratos Crescimento de cravo estandar cv. Nelson, em solo e em substratos Growth of standard carnation cv. Nelson, in soil and substrates

    Directory of Open Access Journals (Sweden)

    Adriana Del Pilar Baracaldo Argüello

    2010-01-01

    Full Text Available El cultivo de clavel en la sabana de Bogotá ha sido importante, ya que representa en la actualidad el segundo lugar de exportación en flores de corte. Recientemente, su producción en sistema de cultivo en sustrato ha aumentado debido fundamentalmente a limitantes de tipo sanitario, por lo cual es importante el estudio del crecimiento de las plantas establecidas en este sistema de cultivo. El objetivo de este estudio fue evaluar el crecimiento del clavel estándar cv. Nelson establecido en sustratos y en suelo, a segundo pico de cosecha, para lo cual, en el SENA - Centro Multisectorial de Mosquera, plantas de clavel se sometieron a los siguientes tratamientos: 100% de cascarilla de arroz quemada; 65% cascarilla de arroz quemada - 35% fibra de coco; 35% cascarilla de arroz quemada - 65% fibra de coco y suelo. En muestreos continuos se evaluaron las variables número de hojas, longitud del tallo, diámetro de tallo, número de nudos y diámetro y longitud del botón floral y se ajustaron curvas de crecimiento. Las plantas cultivadas en el tratamiento suelo presentaron mayor número de tallos. En este tratamiento los tallos florales también presentaron un mayor número de nudos y de hojas; sin embargo, fueron más cortos. Así mismo, presentaron menor diámetro de tallo y menor longitud y diámetro del botón floral; características determinantes de la calidad. Por tanto, la producción de tallos exportables de grado "select" fue significativamente inferior en el tratamiento suelo, en comparación con los demás tratamientos.O cultivo de cravo no planalto de Bogotá tem sido importante, visto que representa, na atualidade, o segundo lugar nas exportações colombianas de flores de corte. Recentemente, sua produção em sistemas de cultivo em substrato tem aumentado em consequência de fatores sanitários. O objetivo deste estudo foi avaliar o desenvolvimento do cravo tipo estandar cv. Nelson, estabelecido em substratos e em solo, na segunda

  12. Preliminary results of the pCT scanner testing at CV-28

    Energy Technology Data Exchange (ETDEWEB)

    Setti, Joao A.P.; Schelin, Hugo R.; Paschuk, Sergei A.; Milhoretto, Edney; Rocha, Rodrigo L. [Federal University of Technology (UTFPR), Curitiba, PR (Brazil)], E-mail: setti@utfpr.edu.br, E-mail: schelin@cpgei.cefetpr.br, E-mail: sergei@utfpr.edu.brj, E-mail: edneymilhoretto@yahoo.com, E-mail: rodrigo.luis.rocha@gmail.com; Ribeiro Junior, Sebastiao [Instituto de Tecnologia para o Desenvolvimento (LACTEC), Curitiba, PR (Brazil)], E-mail: ribeiro@lactec.org.br; Evseev, Ivan G.; Assis, Joaquim T. de; Yevseyeva, Olga [Universidade do Estado do Rio de Janeiro (UERJ), Nova Friburgo, RJ (Brazil). Inst. Politecnico], E-mail: evseev@iprj.uerj.br, E-mail: joaquim@iprj.uerj.br, E-mail: yevseyeva@iprj.uerj.br; Lopes, Ricardo T. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia (COPPE). Lab. de Instrumentacao Nuclear], E-mail: ricardo@lin.ufrj.br; Vinagre Filho, Ubirajara M. [Instituto de Engenharia Nuclear IEN/CNEN-RJ, Rio de Janeiro, RJ (Brazil)], E-mail: bira@ien.gov.br

    2007-07-01

    In the present work the first results obtained with the low energy proton beam of CV-28 at IEN/CNEN and the prototype of a computerized tomography device developed in UFTPR are presented. The system installed in the scatterings chamber of the cyclotron line 3 includes the proton scatter, turntable with translation (1st generation CT scheme), the set of collimators for the secondary proton beam formation and the Si(Li) ORTEC detector. The main parameters of the tomography turntable and the collimators were chosen based on computer simulations with SRIM2006 and GEANT4. A cylindrical glass tube was used as the irradiated sample. On this stage, only the translation of the turntable perpendicular to the proton beam direction was fulfilled. The measured proton energy spectra have, in general, a predicted behavior. However, the experiment revealed some problems with the secondary proton beam formation that should be solved prior to obtain a first pCT image at CV-28. (author)

  13. Plasmalemmal vesicle associated protein (PV1) modulates SV40 virus infectivity in CV-1 cells.

    Science.gov (United States)

    Tse, Dan; Armstrong, David A; Oppenheim, Ariella; Kuksin, Dmitry; Norkin, Leonard; Stan, Radu V

    2011-08-26

    Plasmalemmal vesicle associated protein (Plvap/PV1) is a structural protein required for the formation of the stomatal diaphragms of caveolae. Caveolae are plasma membrane invaginations that were implicated in SV40 virus entry in primate cells. Here we show that de novo Plvap/PV1 expression in CV-1 green monkey epithelial cells significantly reduces the ability of SV40 virus to establish productive infection, when cells are incubated with low concentrations of the virus. However, in presence of high viral titers PV1 has no effect on SV40 virus infectivity. Mechanistically, PV1 expression does not reduce the cell surface expression of known SV40 receptors such as GM1 ganglioside and MHC class I proteins. Furthermore, PV1 does not reduce the binding of virus-like particles made by SV40 VP1 protein to the CV-1 cell surface and does not impact their internalization when cells are incubated with either high or low VLP concentrations. These results suggest that PV1 protein is able to block SV40 infectivity at low but not at high viral concentration either by interfering with the infective internalization pathway at the cell surface or at a post internalization step.

  14. Multi-camera calibration based on openCV and multi-view registration

    Science.gov (United States)

    Deng, Xiao-ming; Wan, Xiong; Zhang, Zhi-min; Leng, Bi-yan; Lou, Ning-ning; He, Shuai

    2010-10-01

    For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the corresponding relationship between each camera view, the computation of the rotation matrix and translation matrix is formulated as a constrained optimization problem. According to the Kuhn-Tucker theorem and the properties on the derivative of the matrix-valued function, the formulae of rotation matrix and translation matrix are deduced by using singular value decomposition algorithm. Afterwards an iterative method is utilized to get the entire coordinate transformation of pair-wise views, thus the precise multi-view registration can be conveniently achieved and then can get the relative positions in them(the camera outside the parameters).Experimental results show that the method is practical in multi-camera calibration .

  15. Heterogeneous compute in computer vision: OpenCL in OpenCV

    Science.gov (United States)

    Gasparakis, Harris

    2014-02-01

    We explore the relevance of Heterogeneous System Architecture (HSA) in Computer Vision, both as a long term vision, and as a near term emerging reality via the recently ratified OpenCL 2.0 Khronos standard. After a brief review of OpenCL 1.2 and 2.0, including HSA features such as Shared Virtual Memory (SVM) and platform atomics, we identify what genres of Computer Vision workloads stand to benefit by leveraging those features, and we suggest a new mental framework that replaces GPU compute with hybrid HSA APU compute. As a case in point, we discuss, in some detail, popular object recognition algorithms (part-based models), emphasizing the interplay and concurrent collaboration between the GPU and CPU. We conclude by describing how OpenCL has been incorporated in OpenCV, a popular open source computer vision library, emphasizing recent work on the Transparent API, to appear in OpenCV 3.0, which unifies the native CPU and OpenCL execution paths under a single API, allowing the same code to execute either on CPU or on a OpenCL enabled device, without even recompiling.

  16. Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO.

    Science.gov (United States)

    Albert, Andreas; Sareedenchai, Vipaporn; Heller, Werner; Seidlitz, Harald K; Zidorn, Christian

    2009-05-01

    Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity of the tissues involved. Factors causing these variations were investigated by climate chamber (CC) experiments focusing on temperature and ultraviolet (UV)-B radiation. Plants of A. montana L. cv. ARBO were grown in CCs under realistic climatic and radiation regimes. Key factors temperature and UV-B radiation were altered between different groups of plants. Subsequently, flowering heads were analyzed by HPLC for their contents of flavonoids and caffeic acid derivatives. Surprisingly, increased UV-B radiation did not trigger any change in phenolic metabolites in Arnica. In contrast, a pronounced increase in the ratio of B-ring ortho-diphenolic (quercetin) compared to B-ring monophenolic (kaempferol) flavonols resulted from a decrease in temperature by 5 degrees C in the applied climate regime. In conclusion, enhanced UV-B radiation is probably not the key factor triggering shifts in the phenolic composition in Arnica grown at higher altitudes but rather temperature, which decreases with altitude.

  17. Health and nutritional status of Wistar rats following subchronic exposure to CV127 soybeans.

    Science.gov (United States)

    Chukwudebe, Amechi; Privalle, Laura; Reed, Andrew; Wandelt, Christine; Contri, Daniela; Dammann, Martina; Groeters, Sibylle; Kaspers, Uwe; Strauss, Volker; van Ravenzwaay, Bennard

    2012-03-01

    This subchronic duration feeding study evaluated the nutritional and health status of rats fed diets containing CV127 at incorporation levels of 11% and 33%. For control comparisons, rats were also exposed to similar incorporation levels of the near isogenic conventional soybean variety (Conquista) and two other conventional soybean varieties (Monsoy, Coodetec). In spite of phenotypic differences among these four soybean varieties, there were no quantitative differences in their respective proximate and other compositional properties, including proteins, amino acids, antinutrients and nutritional cofactors. All diets were prepared by blending the respective processed soybean meal with ground Kliba maintenance meal at high (33%) and low (11%) incorporation levels, and the blended diets were fed to Wistar rats for about 91 days. Although there were some isolated parameters indicating statistically significant changes, these lacked consistency and a plausible mechanism and were thus assessed to be incidental. The totality of results demonstrate that CV127 soybeans are similar with respect to their nutritional value and systemic effects as its near isogenic conventional counterpart, as well as other conventional soybean varieties. Hence, introduction of AHAS gene into soybeans does not substantially alter its compositional properties, nor adversely affect its nutritional or safety status to mammals.

  18. Business Strategy of CV Jaya Sampurna in Facing Soft Drink Distributor Competition in Bekas

    Directory of Open Access Journals (Sweden)

    Seri Nurmala

    2015-11-01

    Full Text Available CV Jaya Sampurna is a soft drink distributor in Bekasi. Distributor company has a low profit margin, moreover the product sold is Fast Moving Consumers Goods with a very low profit margin (3.5%5%. Therefore the company depends on a high volume of sales. Besides, there are many competitors in this industry, thus the business strategy is needed to improve the competitive advantage of the company. Given the conditions, this research was to identify the internal and the external factors, to identify the formulation of business strategy alternative, and to identify the recommendation of functional strategy for CV Jaya Sampurna. Research used descriptive method; and the type of research used was case study. Data were gathered from questionnaires and interview to three member of managers: Director, Operational Manager, and Finance Manager. Data were analyzed by using EFE, IFE, SWOT, IE, Grand Strategy Matrix and QSPM. The result of this research implies that the alternative strategy based on QSPMs result was market penetration

  19. Business Strategy of CV Jaya Sampurna in Facing Soft Drink Distributor Competition in Bekasi

    Directory of Open Access Journals (Sweden)

    Seri Nurmala

    2015-11-01

    Full Text Available CV Jaya Sampurna is a soft drink distributor in Bekasi. Distributor company has a low profit margin, moreover the product sold is Fast Moving Consumers Goods with a very low profit margin (3.5%–5%. Therefore the company depends on a high volume of sales. Besides, there are many competitors in this industry, thus the business strategy is needed to improve the competitive advantage of the company. Given the conditions,this research was to identify the internal and the external factors, to identify the formulation of business strategy alternative, and to identify the recommendation of functional strategy for CV Jaya Sampurna. Research used descriptive method; and the type of research used was case study. Data were gathered from questionnaires and interview to three member of managers: Director, Operational Manager, and Finance Manager. Data wereanalyzed by using EFE, IFE, SWOT, IE, Grand Strategy Matrix and QSPM. The result of this research implies that the alternative strategy based on QSPM’s result was market penetration

  20. Inflorescences production of heliconia cv. Golden Torch under nitrogen and potassium fertilization

    Directory of Open Access Journals (Sweden)

    Márkilla Zunete Beckmann-Cavalcante

    2015-03-01

    Full Text Available The heliconia plants are very demanding in nutrients, especially nitrogen and potassium, but there is a wide variation in their recommendation for fertilization. In this sense the objective was to evaluating the productive attributes of heliconia cv. Golden Torch in function of nitrogen and potassium fertilization in Bom Jesus-PI. The experiment was conductedin the experimental field of Floriculture in Campus Profa. “Cinobelina Elvas” (CPCE at Federal University of Piauí (UFPI in Bom Jesus city at Piauí State and was conducted in factorial 4 x 4 related to: i nitrogen doses (0, 120, 180 and 240 g N hole-1, and ii potassium doses (0, 120, 180 and 240 g K hole-1. Treatments were arranged in a randomized block design with four replications and five rhizomes per share for the evaluation of the three central plants. Were evaluated the days for the first tiller emergence, for the first inflorescence emergence and for inflorescence harvest; plant height, number of flowering stems per plant, diameter of the flowering stem, flowering stem length, inflorescence length and leaf area. For heliconia cv. Golden Torch, potassium application provides increased yield components and it is indicated the application of 120 g K hole-1.

  1. Exogenous application of ascorbic acid alleviates chilling injury in apricot (Prunus armeniaca L. cv. Shahroudi flowers

    Directory of Open Access Journals (Sweden)

    Hassan Bayat

    2013-08-01

    Full Text Available One of the most important limiting factors in spread of apricot in Iran is late spring frost, which damages flower bud and decrease total yield of crop. It has been found that ascorbic acid (AA plays a beneficial role during plant response to chilling and freezing stresses. To evaluate the effects of AA on alleviating of cold stress, the flower buds of Prunus armeniaca L. cv. Shahroudi were sprayed at pink cluster stage with AS at 4 levels (0, 100, 200 and 300 mg. L-1 and were then exposed to artificial cold stress (4 h at –4 °C or without cold stress (+ 25°C. Experimental attributes including electrolyte leakage (EL of flower buds and percentage of damage of pistil, anthers and petals to temperature treatments were determined. The results showed that at - 4°C the lowest and highest percentage of damage and EL of flower buds were observed in application of 200 and 0 mg. L-1 AA, respectively. The highest and lowest percentage of damage of flower organs and EL were obtained in application of 300 and 200 mg. L-1 AA, respectively at + 25 °C. Based on the results of this experiment, AA alleviates the negative effect of cold stress on EL and flower organ damages in apricot cv. Shahroudi, depending on the concentrations of AA used.

  2. Biophenols from Table Olive cv Bella di Cerignola: Chemical Characterization, Bioaccessibility, and Intestinal Absorption.

    Science.gov (United States)

    D'Antuono, Isabella; Garbetta, Antonella; Ciasca, Biancamaria; Linsalata, Vito; Minervini, Fiorenza; Lattanzio, Veronica M T; Logrieco, Antonio F; Cardinali, Angela

    2016-07-20

    In this study, the naturally debittered table olives cv Bella di Cerignola were studied in order to (i) characterize their phenolic composition; (ii) evaluate the polyphenols bioaccessibility; (iii) assess their absorption and transport, across Caco2/TC7. LC-MS/MS analysis has confirmed the presence of hydroxytyrosol acetate, caffeoyl-6'-secologanoside, and comselogoside. In vitro bioaccessibility ranged from 7% of luteolin to 100% of tyrosol, highlighting the flavonoids sensitivity to the digestive conditions. The Caco2/TC7 polyphenols accumulation was rapid (60 min) with an efficiency of 0.89%; the overall bioavailability was 1.86% (120 min), with hydroxytyrosol and tyrosol the highest bioavailables, followed by verbascoside and luteolin. In the cells and basolateral side, caffeic and coumaric acids metabolites, probably derived from esterase activities, were detected. In conclusion, the naturally debittered table olives cv Bella di Cerignola can be considered as a source of bioaccessible, absorbable, and bioavailable polyphenols that, for their potential health promoting effect, permit inclusion of table olives as a functional food suitable for a balanced diet. PMID:27355793

  3. Transient Extremely Soft X-ray Emission from the Unusually Bright CV in the Globular Cluster M3: a New CV X-ray Luminosity Record?

    CERN Document Server

    Stacey, W S; Elsner, R F; Edmonds, P D; Weisskopf, M C; Grindlay, J E

    2011-01-01

    We observed the accreting white dwarf 1E1339.8+2837 (1E1339) in the globular cluster M3 in Nov. 2003, May 2004 and Jan. 2005, using the Chandra ACIS-S detector. The source was observed in 1992 to possess traits of a supersoft X-ray source (SSS), with a 0.1-2.4 keV luminosity as large as 2x10^{35} erg/s, after which time the source's luminosity fell by roughly two orders of magnitude, adopting a hard X-ray spectrum more typical of CVs. Our observations confirm 1E1339's hard CV-like spectrum, with photon index Gamma=1.3+-0.2. We found 1E1339 to be highly variable, with a 0.5-10 keV luminosity ranging from 1.4+-0.3x10^{34} erg/s to 8.5+4.9-4.6x10^{32} erg/s, with 1E1339's maximum luminosity being perhaps the highest yet recorded for hard X-ray emission onto a white dwarf. In Jan. 2005, 1E1339 displayed substantial low-energy emission below 0.3 keV. Although current Chandra responses cannot properly model this emission, its bolometric luminosity appears comparable to or greater than that of the hard spectral comp...

  4. Warm Needling Moxibustion at Zhongji (CV 3) and Zusanli (ST 36) for Urinary Retention after Gynecological Surgery

    Institute of Scientific and Technical Information of China (English)

    Song Xiao-lin; Yu Xin

    2014-01-01

    Objective:To observe the clinical effect of warm needling moxibustion at Zhongji (CV 3) and Zusanli (ST 36) in treatment of urinary retention after gynecological surgery. Methods: Forty patients with urinary retention after gynecological surgery were randomly divided into two groups by the randomized method, 20 cases in each group. Patients in the treatment group were treated by warm needling moxibustion at Zhongji (CV 3) and Zusanli (ST 36). Patients in the control group were treated by inductive urination. The therapeutic effects of the two groups were observed after one course of treatment. Results:The total effective rate was 95.0%in the treatment group and 70.0% in the control group. The difference in the total effective rate between the two groups was statistically significant (P Conclusion: Warm needling moxibustion at Zhongji (CV 3) and Zusanli (ST 36) is more effective than inductive urination in treating urinary retention after gynecological surgery.

  5. Paraneoplastic Choreoathetosis in a Patient with Small Cell Lung Carcinoma and Anti-CRMP5/CV2: A Case Report

    DEFF Research Database (Denmark)

    Lassen, Lisbeth Landschoff; Somnier, Finn; Aydin, Dogu

    2016-01-01

    Introduction: The occurrence of more or less monosymptomatic paraneoplastic choreoathetosis associated with anti-CRMP5/CV2 antibodies is rare. Typically, such autoantibodies are associated with a more classical syndrome - paraneoplastic encephalomyelitis. Frequently, small cell lung carcinoma (SCLC......) is the related neoplastic finding. Case Report: We present a 71-year-old woman who developed visual symptoms with papilledema and chorea. Anti-CRMP5/CV2 antibodies were a feature of both the serum and cerebrospinal fluid. Although SCLC was suspected already at the time of the initial examinations, no signs...... 14 months after the onset of the symptoms. Conclusion: We report paraneoplastic choreoathetosis associated with anti-CRMP5/CV2 antibodies. Such published case histories are rare. Although expected, we did not find any reduced signal intensity at the basal ganglia on the T1-weighted or increased...

  6. Energy saving in Bellota Mexico S.A. de C.V.; Ahorro de energia en Bellota Mexico S.A de C.V.

    Energy Technology Data Exchange (ETDEWEB)

    Salazar Lopez, Jose Albeiro [Bellota Mexico S.A. de C.V. (Mexico)

    2003-07-01

    The present article intends to make a general description of the different activities that were developed in Bellota Mexico S.A. de C.V. during year 2001 and which lead to obtaining the national prize of energy saving granted by the Comision Federal de Electricidad (CFE) and the Fideicomiso para el Ahorro de Energia Electrica (FIDE). In the executed actions stands out an entirely coordinated program that includes: lighting systems, motors, refrigeration systems, transformers, special projects (settling tanks), capacitors, compressed air pipeline, induction furnaces, machinery in general, maximum demand control, preventive maintenance, measuring systems, operational and organizational systems. In all these subjects a fast description is made of the performed actions and the obtained results. It is mentioned in addition the importance of making a power diagnosis and the use of historical data for the planning of the strategies to follow. Also reference is made of the use of appropriate technology that considers the use of high-energy efficiency equipment, such as motors, lamps, compressors, etc. Finally an evaluation is made of the obtained results where the improvement of the electrical parameters stands out such as: reduction of the power index in 35.06%, reduction of the maximum demand in 11.94%, increase of the power factor from 93.53% in 98.21%. The obtained results demonstrate that investing in energy saving it is a good business. [Spanish] El presente trabajo tiene por objeto hacer una descripcion general de las diferentes actividades que se desarrollaron en Bellota Mexico S.A. de C.V. durante el ano 2001 y que condujeron a la obtencion del premio nacional de ahorro de energia otorgado por la Comision Federal de Electricidad (CFE) y el Fideicomiso para el Ahorro de Energia Electrica (FIDE). En las acciones ejecutadas se destaca todo un programa coordinado que abarca: sistemas de iluminacion, motores, sistemas de refrigeracion, transformadores, proyectos

  7. A microsatellite library for Carica papaya L. cv. Sunrise solo Marcadores microssatélites para Carica papaya L. cv. Sunrise solo

    Directory of Open Access Journals (Sweden)

    Silvia Correa Santos

    2003-08-01

    Full Text Available In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8.It was possible the development of the primers only in the library that has utilized the probe (TCA10. This probe allowed the design of 32 primer pairs. From these, 31 presented pattern of unique band in agarose Metaphor and in acrilamide. For primer S36 were observed 2 bands, but with no polymorphism to differentiation in the sexual form at papaya tree culture. However, these primers can be tested, in the futures, in the investigation of the others features in segregated populations of this specie and the related species, germoplasm analysis, cultivars identification, parent evolution and molecular markers for the assisted plant breeding programs.Em áreas experimentais das Fazendas de Ensino e Pesquisa da UNESP/Campus de Ilha Solteira e Jaboticabal foram selecionadas e marcadas 20 plantas hermafroditas e 20 femininas dos cultivares Sunrise Solo, Improved Sunrise Solo cv.72/12 e Baixinho de Santa Amália. As sementes provenientes dos frutos selecionados foram plantadas para analisar-se a eficiência da autofecundação e a freqüência dos sexos nas progênies. Posteriormente, amostras de tecido foliar jovem das plantas matrizes foram coletadas para a extração de DNA. Foram construídas cinco bibliotecas enriquecidas de seqüências microsatélites, utilizando-se as sondas (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8. Foi possível o

  8. Organogênese a partir de segmentos foliares e internodais de videira cv. Merlot Organogenesis from leaf segments and internodes of grapevine cv. Merlot

    Directory of Open Access Journals (Sweden)

    Dayse Cristina de Carvalho

    2011-02-01

    Full Text Available Objetivou-se, neste trabalho, estudar a indução da organogênese em segmentos foliares e internódios de videira cv. Merlot, utilizando diferentes tipos e concentrações de citocininas. O meio de cultura usado foi o MS com redução da metade dos sais e vitaminas, suplementado com 1 µM de ácido naftalenoacético (ANA. Os tratamentos consistiram das concentrações de 2,5; 5,0 ou 10 µM de 6-benzilaminopurina (BAP ou 1-fenil-3-(1,2,3,-tiadiazol-5-il uréia (TDZ. Foram avaliados o porcentual de formação de gemas, calos, oxidação e raízes, além do número de raízes e gemas. Aos 60 dias de cultivo in vitro foi observado que concentrações de 2,5-10 µM de BAP e TDZ permitem que segmentos foliares oxidem e não induzam à oxidação nos internódios. O nível de 5 µM de TDZ favore o maior porcentual de formação de gemas em internódios e segmentos foliares não apresentam capacidade para formar gemas nos níveis de 2,5-10 µM de BAP ou TDZ. O uso de BAP nas concentrações de 2,5 e 5 µM favorece altos porcentuais de enraizamento em internódios. O uso de TDZ no nível de 2,5 µM promove elevada taxa de formação de calos em internódios. Os internódios apresentaram maior capacidade organogênica do que os segmentos foliares.The objective of this work was study the induction of organogenesis on leaf segments and internodes of grapevine cv. Merlot using different types and concentrations of cytokinins. MS half strength culture medium supplemented with 1 µM naftalenoacetic acid (NAA was used. The treatments consisted of the concentrations of 2.5; 5.0 or 10 µM 6-benzilaminopurine (BAP or thydiazuron (TDZ. The percentage of buds, callus, oxidation and roots as well as the number of roots and buds were evaluated. After 60 days of in vitro culture it was observed that the concentrations of 2.5-10 µM BAP or TDZ oxidize leaf segments and do not induce oxidation in the internodes. The level of 5 µM TDZ promote the largest formation of

  9. ORGANIC FERTILIZER AS PHOSPHORUS SUPPLIER FOR Brachiaria brizantha CV. MARANDÚ FERTILIZANTE ORGÂNICO COMO FONTE DE FÓSFORO NO CULTIVO INICIAL DE Brachiaria brizantha CV. MARANDÚ

    Directory of Open Access Journals (Sweden)

    Diego Henriques Santos

    2010-05-01

    Full Text Available

    Phosphorus is considered an essential element for plants, but it is found in low amounts in Brazilian soils, mainly in areas destined to pastures, and the application of organic matter contributes to the improvement of physical, chemical and biological soil attributes. The research had the objective of evaluating the application effects of the Bokashi organic compound on soil macro and micronutrients contents and leaves, as well as on Brachiaria brizantha cv. Marandú dry mass yield, evaluating the potential of the organic fertilizer as phosphorus source, in comparison with conventional chemical fertilizers. Different organic compound doses supplied the soil with 17 kg P2O5 ha-1, 34 kg P2O5 ha-1, 68 kg P2O5 ha-1, 136 kg P2O5 ha-1, and 204 kg P2O5 ha-1, comparing it with the conventional chemical fertilizer and the control. The results revealed that the organic fertilizer Bokashi can adequately replace the conventional chemical fertilizer of Brachiaria brizantha cv. Marandú, supplying the soil and plant with appropriate nutrients levels and maintaining the dry mass yield.

    KEY-WORDS: Organic matter; organic fertilizers; plants mineral nutrition; Bokashi

  10. Chemical and physical studies of type 3 chondrites 12: The metamorphic history of CV chondrites and their components

    Science.gov (United States)

    Guimon, R. Kyle; Symes, Steven J. K.; Sears, Derek W. G.

    1995-01-01

    The induced thermoluminescence (TL) properties of 16 CV and CV-related chondrites, four CK chondrites and Renazzo (CR2) have been measured in order to investigate their metamorphic history. The petrographic, mineralogical and bulk compositional differences among the CV chondrites indicate that the TL sensitivity of the approximately 130 C TL peak is reflecting the abundance of ordered feldspar, especially in chondrule mesostasis, which in turn reflects parent-body metamorphism. The TL properties of 18 samples of homogenized Allende powder heated at a variety of times and temperatures, and cathodoluminescence mosaics of Axtell and Coolidge, showed results consistent with this conclusion. Five refractory inclusions from Allende, and separates from those inclusions, were also examined and yielded trends reflecting variations in mineralogy indicative of high peak temperatures (either metamorphic or igneous) and fairly rapid cooling. The CK chondrites are unique among metamorphosed chondrites in showing no detectable induced TL, which is consistent with literature data that suggests very unusual feldspar in these meteorites. Using TL sensitivity and several mineral systems and allowing for the differences in the oxidized and reduced subgroups, the CV and CV-related meteorites can be divided into petrologic types analogous to those of the ordinary and CO type 3 chondrites. Axtell, Kaba, Leoville, Bali, Arch and ALHA81003 are type 3.0-3.1, while ALH84018, Efremovka, Grosnaja, Allende and Vigarano are type 3.2-3.3 and Coolidge and Loongana 001 are type 3.8. Mokoia is probably a breccia with regions ranging in petrologic type from 3.0 to 3.2. Renazzo often plots at the end of the reduced and oxidized CV chondrite trends, even when those trends diverge, suggesting that in many respects it resembles the unmetamorphosed precursors of the CV chondrites. The low-petrographic types and low-TL peak temperatures of all samples, including the CV3.8 chondrites, indicates metamorphism

  11. Facing the differences between Facebook and OpenCV : A facial detection comparison between Open Library Computer Vision and Facebook

    OpenAIRE

    Blomgren, Staffan; Hertz, Marcus

    2015-01-01

    Face detection is used in many different areas and with this thesis we aim to show the difference between Facebooks face detection soft-ware compared with an open source version from OpenCV. By using the simplest implementation of OpenCV we want to find out if it is viable for use in personal applications and be of help for others wanting to implement face detection. The dataset was meticulously checked to find the exact number of faces in each image so that the optimal result is given. The c...

  12. The effect of vowel height on Voice Onset Time in stop consonants in CV sequences in spontaneous Danish

    DEFF Research Database (Denmark)

    Mortensen, Johannes; Tøndering, John

    2013-01-01

    Voice onset time has been reported to vary with the height of vowels following the stop consonant. This paper investigates the effects of vowel height on VOT in Danish CV sequences with stop consonants in Danish spontaneous speech. A significant effect of vowel height on VOT was found in the unas......Voice onset time has been reported to vary with the height of vowels following the stop consonant. This paper investigates the effects of vowel height on VOT in Danish CV sequences with stop consonants in Danish spontaneous speech. A significant effect of vowel height on VOT was found...

  13. Whole‐rock 26Al‐26Mg systematics of amoeboid olivine aggregates from the oxidized CV3 carbonaceous chondrite Allende

    DEFF Research Database (Denmark)

    Olsen, Mia Bjørg Stolberg; Krot, Alexander N.; Larsen, Kirsten K.;

    2011-01-01

    Abstract– We report on mineralogy, petrography, and whole-rock 26Al-26Mg systematics of eight amoeboid olivine aggregates (AOAs) from the oxidized CV chondrite Allende. The AOAs consist of forsteritic olivine, opaque nodules, and variable amounts of Ca,Al-rich inclusions (CAIs) of different types....... (2008). In contrast, whole-rock 26Al-26Mg isotope systematics of CAIs and AOAs from the reduced CV chondrite Efremovka define a single isochron with a slope of (5.25± 0.01) × 10−5 (Larsen et al. 2011). We infer that the excesses in 26Mg* present in Allende AOAs are due to their late-stage open...

  14. Sonication inhibited browning but decreased polyphenols contents and antioxidant activity of fresh apple (malus pumila mill, cv. Red Fuji) juice.

    Science.gov (United States)

    Sun, Yujing; Zhong, Liezhou; Cao, Lianfei; Lin, Wenwen; Ye, Xingqian

    2015-12-01

    Enzyme browning is the main challenge in the preparation of fresh apple juice. The influence of sonication on browning, as well as polyphenols and antioxidant activity of fresh apple juice was investigated. It was found that ultrasound can inhibit the browning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice, but decreased the contents of total phenolic content (TPC), total flavonoid content (TFC) and chlorogenic acid and reduced the antioxidant activity. On the whole, ultrasound technology cannot be used to the antibrowning of fresh apple (Malus pumila Mill, cv. Red Fuji) juice.

  15. Temperatura, humedad relativa y luz para almacenamiento de tallos florales de Heliconia psittacorum L. f. cv. Trópica.

    OpenAIRE

    Bañuelos Hernández, Karina Patricia

    2012-01-01

    La especie Heliconia psittacorum L. f. cv. Trópica se ha identificado en los últimos años como una ornamental tropical con gran demanda debido a su belleza y a su vida postcosecha prolongada, que puede superar los 15 días; sin embargo, se desconoce el manejo postcosecha adecuado durante su comercialización. El objetivo de este estudio fue identificar las condiciones adecuadas para el almacenamiento postcosecha de tallos florales de H. psittacorum L. f. cv. Trópica. Se postula que la combina...

  16. Expression of Disease Resistance Related Genes in Banana Seedling Induced by Bacillus subtilis Strain TR21%枯草芽孢杆菌TR21对香蕉抗病相关基因表达的诱导作用

    Institute of Scientific and Technical Information of China (English)

    喻国辉; 周林; 程萍; 黎永坚; 杨紫红

    2012-01-01

    广谱抗真菌枯草芽孢杆菌Bacillus subtilis菌株TR21在温室和大田对香蕉枯萎病具有较好的防效,其机制已证明与诱导香蕉产生系统抗性有关。本文以巴西蕉(Musa AAA Cavendish subgroup cv.Brazil)为材料,利用半定量RT-PCR法,以香蕉25S rRNA基因为内标,研究灌根接种菌株TR21后对香蕉根系4种抗病相关基因表达的影响。结果表明,PAL、POD、PR-3和PR-1基因在接种后表达水平均表现上调趋势,但PAL和POD基因的表达增幅明显高于PR-3和PR-1基因。PAL和POD基因在接种后12 h表达量最高,与TR21在香蕉根部的定殖规律表现出一致性。系统诱导抗性是枯草芽孢杆菌TR21防治香蕉枯萎病的机制之一。%Bacillus subtilis strain TR21 with broad-range antifungal activity has shown potential to control banana Fusarium wilt in greenhouse and fields.The mechanism through which strain TR21 confer resistance to the disease has previously been demonstrated to involve induced resistance.In this study,with Musa 25S rRNA gene as an internal control,the effect of strain TR21 on expression of four defense-related genes in roots of Musa AAA Cavendish subgroup cv.Brazil was investigated by semi-quantitative RT-PCR method.Results showed that the expression of PAL,POD,PR-3 and PR-1 was up-regulated after being inoculated with strain TR21,but PAL and POD had higher increase amplitude.The expression of PAL and POD reached the maximum in roots at 12 h after inoculation,which was in accordance with colonization of strain TR21 in roots of the plant.It suggested that induced systemic resistance was one of antagonistic mechanisms of the strain TR21 against banana Fusarium wilt.

  17. OpenCV-Based Nanomanipulation Information Extraction and the Probe Operation in SEM

    Directory of Open Access Journals (Sweden)

    Dongjie Li

    2015-02-01

    Full Text Available Aimed at the established telenanomanipulation system, the method of extracting location information and the strategies of probe operation were studied in this paper. First, the machine learning algorithm of OpenCV was used to extract location information from SEM images. Thus nanowires and probe in SEM images can be automatically tracked and the region of interest (ROI can be marked quickly. Then the location of nanowire and probe can be extracted from the ROI. To study the probe operation strategy, the Van der Waals force between probe and a nanowire was computed; thus relevant operating parameters can be obtained. With these operating parameters, the nanowire in 3D virtual environment can be preoperated and an optimal path of the probe can be obtained. The actual probe runs automatically under the telenanomanipulation system's control. Finally, experiments were carried out to verify the above methods, and results show the designed methods have achieved the expected effect.

  18. Defect Detection in Superconducting Radiofrequency Cavity Surface Using C + + and OpenCV

    Science.gov (United States)

    Oswald, Samantha; Thomas Jefferson National Accelerator Facility Collaboration

    2014-03-01

    Thomas Jefferson National Accelerator Facility (TJNAF) uses superconducting radiofrequency (SRF) cavities to accelerate an electron beam. If theses cavities have a small particle or defect, it can degrade the performance of the cavity. The problem at hand is inspecting the cavity for defects, little bubbles of niobium on the surface of the cavity. Thousands of pictures have to be taken of a single cavity and then looked through to see how many defects were found. A C + + program with Open Source Computer Vision (OpenCV) was constructed to reduce the number of hours searching through the images and finds all the defects. Using this code, the SRF group is now able to use the code to identify defects in on-going tests of SRF cavities. Real time detection is the next step so that instead of taking pictures when looking at the cavity, the camera will detect all the defects.

  19. Proliferation Potential of 18-Month-Old Callus of Ananas comosus L. cv. Moris

    Directory of Open Access Journals (Sweden)

    A.E. De Silva

    2006-01-01

    Full Text Available Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of Ananas comosus L. cv. Moris were assessed in vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS media, 32.22 μM α-naphthaleneacetic acid (NAA gave the highest mean fresh weight of callus (46.817 g. Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D was inferior to NAA, while b-naphthoxy acetic acid (BNOA and p-chlorophenoxy acetic acid (4-CPA were not effective in proliferating 18-months old callus. Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus. However, in terms of callus production, NAA at 32.22 μM was economically better.

  20. Use of nootkatone as a senescence indicator for Rouge La Toma Cv. grapefruit (Citrus paradisi Macf.).

    Science.gov (United States)

    Biolatto, Andrea; Sancho, Ana M; Cantet, Rodolfo J C; Güemes, Daniel R; Pensel, Norma A

    2002-08-14

    The objective of this research was to study the usefulness of nootkatone as a senescence indicator for Rouge La Toma cv. grapefruit (Citrus paradisi Macf.), simulating different treatments that included the normal postharvest handling of citrus fruits: temperature conditioning, cold storage, shipment periods to overseas markets such as Japan and the U.S., marketing conditions, and storage at nonchilling temperature (control treatments). The highest nootkatone levels, determined by GLC-MS analyses, were detected in fruits subjected to control treatments. No significant differences were observed in nootkatone levels between treatments either with or without temperature conditioning prior to the start of the cold storage. Levels of nootkatone increased throughout time for all assayed treatments. The linear regressions of nootkatone levels showed correlation coefficients of 0.80 and 0.83 with storage time (29 and 42 days, respectively). Therefore, nootkatone appears to be a good indicator of senescence for Rouge La Toma grapefruit. PMID:12166965

  1. Factors affecting taste scores of early season seedless table grape cv. Mystery and Prime.

    Science.gov (United States)

    Sonego, Lilian; Lurie, Susan; Zuthi, Yohanan; Kaplonov, Tatiana; Ben-Arie, Ruth; Kosto, Itzhak

    2002-01-30

    Table grapes of cv. Mystery and Prime were harvested from 10 farms in two growing areas of Israel over two seasons. The grapes were separated on the basis of sucrose solutions from 12 to 18%; soluble solids content (SSC), titratable acidity (TA), and pH were determined; and taste tests were conducted. SSC gave the best correlation with taste tests, and multiple regression of SSC, TA, and pH improved the correlation. There were both seasonal and regional differences in the measured maturity parameters. Lower TA and higher pH were found in grapes from the Jordan Valley. Volatiles were predominantly C(6) compounds hexanal and 2-hexanal, contributing a fresh aroma to the grapes. It is concluded that Mystery and Prime grapes have good organoleptic quality if harvested at SSC levels of >14%. PMID:11804527

  2. Analysis of ultraviolet atmospheric eclipses in the Wolf-Rayet binary CV Serpentis

    Science.gov (United States)

    Eaton, J. A.; Cherepashchuk, A. M.; Khaliullin, Kh. F.

    1985-01-01

    While no eclipses deeper than 0.04 mag are noted in the present UV spectra, covering one-half of an orbital cycle of CV Ser, in the electron scattering continuum at 2400-3200 A or in fine error sensor observations, marked atmospheric eclipses of up to 0.5 mag depth are observed in individual strong lines and over large ranges of the continuum at shorter wavelengths. The flux above the continuum in the C II 1247 A, Si IV 1400 A, and Si IV 1723 lines showed similar phase dependence with emission weakening, as well as with the emission's going into absorption as phase progresses from superior to inferior conjunction of the WC star (primary eclipse). These observations show effects very similar to the behavior of WN stars in the UV.

  3. Entorno de programación gráfico OpenCV

    OpenAIRE

    Chamizo Alberto, Daniel

    2013-01-01

    El objetivo del trabajo de fin de grado es el diseño e implementación de un entorno gráfico para que un usuario pueda desarrollar algoritmos de visión por computador. Se pretende que el entorno gráfico sea fácil e intuitivo para que el usuario pueda trabajar con las librerías OpenCV de una manera práctica y rápida. Tendrá implementado unas funciones básicas de dichas librerías y una vez terminadas, se dejarán en código abierto para que se puedan mejorar en un futuro. El ento...

  4. Tutoriál práce s OpenCV

    OpenAIRE

    Běhal, Lukáš

    2010-01-01

    Tato bakalářská práce se zabývá identifikací a popisem základní činnosti doposud nezdokumentovaných metod v knihovně OpenCV. Konkrétně jsou zde popsány algoritmy FAST corner detector, Maximally stable extremal regions, LDetector, HOG people and object detector a One-way descriptor. Každá metoda obsahuje teoretickou část, deklaraci funkcí s popisem jejich parametrů a v neposlední řadě rovněž příklad použití. Pro každou z metod byla také vytvořena vzorová demo aplikace. This bachelor thesis ...

  5. TS-CV contracts for the LHC case study for the TS workshop 2004

    CERN Document Server

    Inigo-Golfin, J; CERN. Geneva. TS Department

    2004-01-01

    The cooling and ventilation group deals with a great number of supplies and installation contracts, as well as some service ones, in relation to the LHC project. In the course of the last years the group has increased its experience in the handling of these contracts in particular as a consequence of the difficult situations sometimes faced, which have been trying but enriching. This paper reviews some statistics on the performance of the CV contracts and draws some conclusions from a set of key cases. These cases can be helpful in identifying possible weak points during the different project phases (engineering, tendering and contracting, installation, etc.) and can provide a basis for the discussion of potential solutions.

  6. Humudifucol and Bioactive Prenylated Polyphenols from Hops (Humulus lupulus cv. "Cascade").

    Science.gov (United States)

    Forino, Martino; Pace, Simona; Chianese, Giuseppina; Santagostini, Laura; Werner, Markus; Weinigel, Christina; Rummler, Silke; Fico, Gelsomina; Werz, Oliver; Taglialatela-Scafati, Orazio

    2016-03-25

    Humulus lupulus (hop plant) has long been used in traditional medicine as a sedative and antimicrobial agent. More recently, attention has been devoted to the phytoestrogenic activity of the plant extracts as well as to the anti-inflammatory and chemopreventive properties of the prenylated chalcones present. In this study, an Italian sample of H. lupulus cv. "Cascade" has been investigated and three new compounds [4-hydroxycolupulone (6), humudifucol (7) and cascadone (8)] have been purified and identified by means of NMR spectroscopy along with four known metabolites. Notably, humudifucol (7) is the first prenylated dimeric phlorotannin discovered in nature. Because structurally related phloroglucinols from natural sources were found previously to inhibit microsomal prostaglandin E2 synthase (mPGES)-1 and 5-lipoxygenase (5-LO), the isolated compounds were evaluated for their bioactivity against these pro-inflammatory target proteins. The prenylated chalcone xanthohumol inhibited both enzymes at low μM concentrations. PMID:26918635

  7. The fractionation of noble gases in diamonds of CV3 Efremovka chondrite

    Science.gov (United States)

    Fisenko, A. V.; Verchovsky, A. B.; Semjonova, L. F.; Shukolyukov, Yu. A.

    1993-01-01

    It was shown that in diamonds of Efremovka CV3 the noble gases with normal isotopic compositions are fractionated in different degree while the correlation of isotopic anomalous components is nearly constant. Some data for noble gases in DE-4 sample of Efremovka chondrite are considered. In contrast to DE-2 sample the DE-4 was treated except conc. HClO4, 220 C in addition with mixture of conc. H2SO4+H3PO4 (1:1), 220 C, twice. Noble gases analysis were performed in Germany at Max Plank Institute fur Chemie. Noble gases were released by oxidation of samples at stepped heating from 420 C to 810 C and by pyrolysis at 580, 590, and 680 C.

  8. Clinical Application of Shenque (CV 8) plus Eight Battle Points for Obesity

    Institute of Scientific and Technical Information of China (English)

    LIU Xiao-hui; HANG Guo-qi

    2007-01-01

    In order to observe the therapeutic effect of Eight Battle Points for simple obesity.Methods:In the treatment of 98 cases of the patients with obesity by the oblique puncture of long needles into Eight Battle Points,the body weight,body weight index (BWI),waist circumference,buttock circumference and fat distribution rate of the patients were measured and assessed respectively before and after the treatments.And the changes of complications of obesity were observed before and after the treatments.Results:There were significant differences (P<0.05) in various indexes of obesity before and after acupuncture treatment.Conclusion:Acupuncture by puncturing Shenque(CV 8) plus Eight Battle Points is positive in the treatment of obesity and has a better therapeutic effect for complications of obesity.

  9. Differences in Fruit Quality of Strawberry cv. Elsanta Depending on Cultivation System and Harvest Time

    Directory of Open Access Journals (Sweden)

    Sandra Voća

    2007-12-01

    Full Text Available The aim of this research was to determine differences in chemical composition of strawberry cv. Elsanta fruit in three different cultivation systems and at three harvest times. Th e cultivation systems were: open field, high tunnel and hydroponics, all located in the Zagreb area. Th e harvest times were: the beginning, the middle and the end of strawberry harvest season. In the harvested fruit following parameters were determined: dry matter, total soluble solids (ºBrix, total acid (TA, Brix/TA, pH, L-ascorbic acid, natural invert and total invert. Th ere were significant differences between high tunnel, open field and hydroponics cultivation. Chemical parameters were somewhat highest for fruit grown in high tunnel, and only values of total invert were higher in open field at second and third harvest time. Based on statistical analysis fruit grown in high tunnel had good quality at all three harvest times.

  10. Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’

    Directory of Open Access Journals (Sweden)

    Kamnoon Kanchanapoom

    2010-01-01

    Full Text Available A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’ was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on ¼MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.

  11. Development of seedlings of watermelon cv. Crimson Sweet irrigated with biosaline water

    Directory of Open Access Journals (Sweden)

    José E. S. B. da Silva

    2015-09-01

    Full Text Available ABSTRACTThe limited access and the scarcity of good quality water for agriculture are some of the major problems faced in agricultural areas, particularly in arid and semiarid regions. The aim of this study was to evaluate the quality of watermelon seedlings (cv. Crimson Sweet, irrigated with different concentrations of biosaline water of fish culture. The experimental design was completely randomized with five treatments, corresponding to biosaline water at different concentrations (0, 33, 50, 67 and 100%, and four replicates of 108 seedlings. Watermelon seeds were sown in plastic trays filled with commercial substrate and irrigated with different solutions of biosaline water. Seedlings were harvested for biometric analysis at 14, 21 and 28 days after sowing. The use of biosaline water did not affect emergence and establishment of seedlings until 14 days after sowing, the period recommended for transplantation. However, the use of biosaline water affected the development of seedlings with longer exposure time.

  12. Central heating pipes cause unwanted heating; CV-leidingen zorgen voor ongewenste opwarming

    Energy Technology Data Exchange (ETDEWEB)

    Wessels, R. [biq-stadsontwerp, Rotterdam (Netherlands); Nuijten, O. [ISSO, Rotterdam (Netherlands)

    2011-12-15

    Research has shown that the risk of hot spots in the drinking water pipes is very high. Hot spots are, for example, caused by central heating pipes that are too close to the water pipes. The water pipes may be 25 C for a long period, thus creating the risk of legionella growth. The various disciplines need to be careful in the design stage and building stage to prevent such situations from occurring. [Dutch] Onderzoek heeft uitgewezen dat het risico op 'hotspots' in de drinkwaterleidingen erg groot is. Hotspots worden bijvoorbeeld veroorzaakt door cv-leidingen die te dicht in de buurt van waterleidingen lopen. Die waterleidingen kunnen dan langdurig warmer zijn dan 25C en daardoor gevaar opleveren voor legionellagroei. Het vereist zorg van meerdere disciplines in de ontwerpfase en de bouwfase om deze situaties te vermijden.

  13. Inducing gravitropic curvature of primary roots of Zea mays cv Ageotropic

    Science.gov (United States)

    Moore, R.; Evans, M. L.; Fondren, W. M.

    1990-01-01

    Primary roots of the mutant 'Ageotropic' cultivar of Zea mays are nonresponsive to gravity. Their root caps secrete little or no mucilage and touch the root only at the extreme apex. A gap separates the cap and root at the periphery of the cap. Applying mucilage from normal roots or substances with a consistency similar to that of mucilage to tips of mutant roots causes these roots to become strongly graviresponsive. Gravicurvature stops when these substances are removed. Caps of some mutants secrete small amounts of mucilage and are graviresponsive. These results indicate that (a) the lack of graviresponsiveness in the mutant results from disrupting the transport pathway between the cap and root, (b) movement of the growth-modifying signal from the cap to the root occurs via an apoplastic pathway, and (c) mucilage is necessary for normal communication between the root cap and root in Zea mays cv Ageotropic.

  14. Obtaining non-suckering plants of hazelnut cv Tonda Gentile delle Langhe by gamma-radiation

    International Nuclear Information System (INIS)

    Hazelnut (Corylus avellana L.) main cultivars form a large number of crown suckers. Also cv Tonda gentile delle Langhe (TGL) grown in Piemonte region (Italy) shows this negative habit. Through crossing with non-suckering species of Corylus, plants have been obtained with the same character, which can be used as rootstocks. In our Institute we tried to create selections of TGL without suckers by means of induced mutagenesis. 500 plants of this cultivar were treated with gamma rays at 8 kR, with the aim to hit the crown zone where adventitious buds are formed. Suckers arising from these buds were isolated and transplanted in number of 1150. Six of them did not form any suckers in five years of observations. Morphological and vegetative characters of these mutants are described. (author)

  15. Armazenamento de caqui (Diospyros kaki, L.) cv. Quioto, em atmosfera controlada

    OpenAIRE

    DONAZZOLO JOEL; BRACKMANN AURI

    2002-01-01

    Objetivou-se avaliar, com o presente trabalho, o efeito da temperatura e do CO2 no armazenamento em atmosfera controlada sobre a conservação de caqui cv. Quioto. Foram avaliadas as temperaturas -1,0 e -0,5ºC, e pressões parciais de CO2 de 0; 5 e 10kPa, com os tratamentos arranjados em um esquema bifatorial. Os frutos foram avaliados após 3 meses de armazenamento mais 3 dias de exposição à temperatura ambiente (18-20ºC). Conforme os resultados, não foi constatada interação entre os fatores, ha...

  16. Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026

    Directory of Open Access Journals (Sweden)

    Ahmad Humayan KABIR

    2010-12-01

    Full Text Available Quorum sensing signals are referred to as acylated homoserine lactones (AHL that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10 media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate.

  17. EFEITO DO ÁCIDO INDOLBUTÍRICO NO ENRAIZAMENTO IN VITRO DE KIWI, CV. HAYWARD

    Directory of Open Access Journals (Sweden)

    Sérgio Lucemar Bonorino Figueiredo

    1994-12-01

    Full Text Available O objetivo deste trabalho foi estudar o efeito de diferentes concentrações de ácido indolbutírico (AIB no enraizamento in vitro de kiwi (Actinidia deliciosa, cv. Hayward. O material vegetal utilizado foi proveniente da coleção do Laboratório, de onde retiraram-se explantes, da parte aérea, sem ápice caulinar e com um par de folhas. O AIB foi utilizado através da imersão da base das estacas em solução aquosa, nas concentrações de 0, 10, 20 e 40mg/l. Os explantes permaneceram nesta solução nos seguintes tempos: imersão rápida (5 segundos, 1, 2 e 4 horas. Após os tratamentos, as estacas foram colocadas em meio de cultura MS 50%, acrescido de sacarose, mio-inositol e ágar. Verificou-se que o AIB não teve efeito positivo sobre o número de raízes primárias e comprimento da raiz principal. Verificou-se que, nas concentrações mais elevadas (20 e 40mg/l de AIB e durante a imersão por 4 horas, houve excessiva formação de calos, ocasionando deformação das estacas. Nas condições em que o presente trabalho foi realizado, concluiu-se que não há necessidade de utilização do AIB para o enraizamento in vitro de kiwi, cv. Hayward.

  18. The Rosa chinensis cv. Viridiflora Phyllody Phenotype Is Associated with Misexpression of Flower Organ Identity Genes.

    Science.gov (United States)

    Yan, Huijun; Zhang, Hao; Wang, Qigang; Jian, Hongying; Qiu, Xianqin; Baudino, Sylvie; Just, Jeremy; Raymond, Olivier; Gu, Lianfeng; Wang, Jihua; Bendahmane, Mohammed; Tang, Kaixue

    2016-01-01

    Phyllody is a flower abnormality in which leaf-like structures replace flower organs in all whorls. Here, we investigated the origin and the molecular mechanism of phyllody phenotype in Rosa chinensis cv. Viridiflora, an ancient naturally occurring Chinese mutant cultivar. Reciprocal grafting experiments and microscopy analyses, demonstrated that the phyllody phenotype in Viridiflora is not associated with phytoplasmas infection. Transcriptome comparisons by the mean of RNA-Seq identified 672 up-regulated and 666 down-regulated genes in Viridiflora compared to its closely related genotype R. chinensis cv. Old Blush. A fraction of these genes are putative homologs of genes known to be involved in flower initiation and development. We show that in flower whorl 2 of Viridiflora, a down-regulation of the floral organ identity genes RcPISTILLATA (RcPI), RcAPETALA3 (RcAP3) and RcSEPALLATA3 (RcSEP3), together with an up-regulation of the putative homolog of the gene SUPPRESSOR of OVEREXPRESSION of CONSTANS1 (RcSOC1) are likely at the origin of the loss of petal identity and leaf-like structures formation. In whorl 3 of Viridiflora, ectopic expression of RcAPETALA2 (RcAP2) along with the down regulation of RcPI, RcAP3, and RcSEP3 is associated with loss of stamens identity and leaf-like structures formation. In whorl 4, the ectopic expression of RcAP2 associated with a down-regulation of RcSEP3 and of the C-class gene RcAGAMOUS correlate with loss of pistil identity. The latter also suggested the antagonist effect between the A and C class genes in the rose. Together, these data suggest that modified expression of the ABCE flower organ identity genes is associated with the phyllody phenotype in the rose Viridiflora and that these genes are important for normal flower organs development. PMID:27462328

  19. RECUPERAÇÃO DE SALMONELLA SPP. EM MANGA (MANGIFERA INDICA CV. TOMMY ATKINS

    Directory of Open Access Journals (Sweden)

    Silvana Belém de Oliveira Vilar

    2015-06-01

    Full Text Available Salmonella spp. é um patógeno que se destaca como agente responsável por doenças veiculadas por alimentos. Alguns estudos demonstram que o consumo de manga contaminada foi responsável por alguns destes surtos. Este trabalho objetivou investigar a eficiência de diferentes metodologias para a recuperação de Salmonella em manga (Mangifera indica cv. Tommy Atkins. Para o estudo, as mangas foram artificialmente contaminadas com um pool de Salmonella contendo culturas de Salmonella Typhimurium, Salmonella Enteritidis e Salmonella Brasil. Foram ccomparados dois métodos de recuperação: Esfregaço de Superfície (Swab e Fragmento do Fruto. A contagem foi realizada de duas maneiras: por Semeadura em Superfície em meio MLCB e pela metodologia de Duas Camadas de Ágar (ágar não seletivo + sobrecamada de ágar seletivo utilizando-se neste caso os meios de cultura TSA +MLCB ou TSA + XLD. Após análise estatística dos dados obtidos por Análise de Variância e Teste de Tukey observou-se que houve diferenças e que os melhores resultados foram obtidos quando se utilizou a combinação do Fragmento do Fruto, com a recuperação nos meios TSA + MLCB. Concluiu-se que esse método foi eficiente na recuperação de cepas de Salmonella artificialmente inoculadas em mangas cv. Tommy Atkins no estádio de maturação 2.

  20. Efficient Axillary Shoot Proliferation and in Vitro Rooting of Apple cv. ‘Topaz’

    Directory of Open Access Journals (Sweden)

    Snjezana KERESA

    2012-05-01

    Full Text Available ‘Topaz’ is a modern Czech apple cultivar well accepted by consumers and scab-resistant, providing reasons for the significant spreadof cv. ‘Topaz’ in European orchards, especially in the organic fruit production industry. Growing the apple trees on their own rootsprovides some advantages in comparison with grafted trees. Micropropagation is the method of choice for plantlet production for thispurpose as well as for the establishment of healthy mother stock trees as a source of scions. The efficiency of axillary shoot proliferationwas examined on four media differing in plant growth regulators and their concentrations, and from three explant types: intact ordecapitated and defoliated microshoots placed vertically and one-nodal segments placed horizontally. All media consisted of Quoirin andLepoivre (QL macroelements and Murashige and Skoog (MS microelements. Furthermore, rooting efficiency on six different media/treatments was analyzed. Media with 1 mg/L 6-benzylaminopurine (BA or BA (0.5 mg/L + 1.5 mg/L kinetin (Kin produced similarnumber of microshoots per inoculated one (2.5 and 2.4, respectively. Medium with 1 mg/L thidiazuron (TDZ produced significantlyhigher number of shoots (3.6 but they were fasciated. Three different explant types also produced similar numbers of microshoots.High rooting efficiency (68.7%, a high number of roots per shoot (6.6 and the best quality of shoots were obtained in rooting mediumcontaining 2 mg/L of indole-3-butyric acid (IBA. An efficient method of shoot proliferation was established, and, since rooting was themost critical step, an efficient procedure for rooting apple cv. ‘Topaz’ was established.

  1. Economic valuation of the Seto Inland Sea by using an Internet CV survey.

    Science.gov (United States)

    Tsuge, Takahiro; Washida, Toyoaki

    2003-01-01

    We estimate the economic value of the natural environment damaged in the Seto Inland Sea after the introduction of the Law on Temporary Measures for the Environmental Conservation of the Seto Inland Sea (Setouchi Law) and the value of the natural environment that survived, using a Contingent Valuation (CV) survey on an Internet web site. The CV survey contains three plans. Plan 1 is to restore 4 ha of reclaimed land. By estimating the Willingness To Pay (WTP) for plan 1, we can appraise the value of the natural environment that was damaged as a result of the original reclamation. Plan 2 is to transplant Zostera (eel-grass) into an area of 10 ha offshore. Plan 3 is to preserve the shore area, a natural habitat for rare animal species, under the National Trust Program. From the WTP for plans 2 and 3, we can estimate the value of the shore area and the areas a little farther offshore. The value of the natural environment damaged in the Seto Inland Sea as a result of reclaiming projects after the introduction of the Setouchi Law and the value of the existing natural environment of the Seto Inland Sea from the WTP for the plans were estimated to about 172 trillion yen (1.46 trillion dollars) and about 424 trillion yen (3.60 trillion dollars), respectively. The results indicate that in the 25 years since the introduction of the Setouchi Law, we have degraded every year about 6.88 trillion yen (58.5 billion dollars) worth of the natural environment by reclaiming. Some seaweed farms and natural shore areas, natural habitats to rare marine life-forms like the horseshoe crab and the fiddler crab have survived, but their value amounts to about 80% of Japan's GDP.

  2. Quantifying key parameters as elicitors for alternate fruit bearing in cv. 'Elstar' apple trees.

    Science.gov (United States)

    Krasniqi, Anne-Lena; Damerow, Lutz; Kunz, Achim; Blanke, Michael M

    2013-11-01

    The commonly known alternate bearing, i.e. year-to-year change of large and small yields of fruit tree crops worldwide, is often induced by abiotic stress such as late frost, which will eliminate flowers or fruitlets. This study presents an alternative form, biotic biennial bearing, i.e. change of large and small yields of the same trees within the same tree row in the same year. Three methods were developed or modified for the analysis of the number of flower clusters and yield of 2086 apple (Malus domestica Borkh.) cv. 'Elstar' trees. The first method, i.e., based on intersect between yield in year x and year x+1 and flower clusters in year x, yielded 91-106 flower clusters, whereas the second method, i.e., mean yield in year x and year x+1, resulted in a range of 72-133 flower clusters, or 9.6kg/tree necessary for sustainable cultivation of apple cv. 'Elstar'. The third 'biennial bearing index' (BBI), was calculated in three ways as the ratio of differences in tree yields to cumulative tree yield, for individual trees (rather than orchard average) to demonstrate the tree-to-tree alternation. A scheme for the possible underlying regulatory mechanisms was developed, which includes potential elicitors such as light deprivation and subsequent lack of flower initiation, are discussed as a possible result of polar basipetal GA7 transport, cytokinin level in the xylem and phloem and down-regulation of the gene expression of the flowering gene. Suggested countermeasures included early chemical or mechanical thinning.

  3. Uso eficiente del agua para el cultivo de rosa cv. Freedom bajo invernadero

    Directory of Open Access Journals (Sweden)

    John J. Arévalo-H

    2013-08-01

    Full Text Available Determinar el requerimiento de agua y el momento adecuado de distribución, es un factor fundamental para el cultivo de flores. El objetivo del presente trabajo fue determinar el efecto de la aplicación de tres láminas de riego sobre el desarrollo y producción del cultivo de la Rosa cv. Freedom, bajo invernadero. El experimento se realizó con tres láminas de riego en un cultivo de rosa (Rosa spp. cv. Freedom. Se analizó el crecimiento de los tallos florales, la vida en florero, la producción y la eficiencia en el uso del agua. Los tratamientos se basaron en tres niveles de evapotranspiración de cultivo (ETc (70, 80 y 100%. Durante el estudio, el cultivo se mantuvo en producción constante. Se aplicaron 384,87, 254,25 y 218,97 m³ correspondiente a 100, 80 e 70% de ETc respectivamente, significando un ahorro de agua de 14,0 y 21,8% en el 80 e 70% de ETc respectivamente. El coeficiente de cultivo (Kc vario entre 0,58 y 1,65, con una media de 1,13. No se encontraron diferencias en la producción. Las curvas de crecimiento presentaron incremento continuo, con ajuste al modelo logístico. El 70% de ETc presentó la mayor eficiencia en el uso del agua, con 185 tallos m-3. La conservación media de los tallos florales fue de 12 a 15 días. La mayor causa de pérdida fue la deshidratación, que inició en el día 11, comportamiento normal del tallo floral.

  4. Rooting and acclimatization of the Japanese plum tree, cv. América

    Directory of Open Access Journals (Sweden)

    Juliana de Magalhães Bandeira

    2012-06-01

    Full Text Available Rooting and acclimatization are limiting steps in plant micropropagation, especially in woody plant species. This study aimed to evaluate the IAA and IBA effect on the in vitro rooting and acclimatization of micropropagated shoots of Japanese plum (Prunus salicina Lindl. cv. América. Shoots from 3 to 4 cm long were inoculated in MS medium with half salt and vitamin concentrations (MS/2 added with IAA and IBA (0, 0.25, 0.5, 0.75 and 1 mg L-1. After a 20-day period in in vitro cultivation, the shoots were evaluated, and then transferred to a greenhouse, and evaluated after 30 days. At the end of the in vitro cultivation period, no significant interactions were observed for number of roots per shoot and rooting percentage, but a significant effect was recorded for auxin type only, for which shoots grown in media added with IBA showed high values - 0.87 and 41.95%, respectively. A linear increase response from 1.45 to 5.75 cm was verified for root length of shoots cultivated in IBA medium; however, no significant effect was observed, and a 0.86 cm average root length per shoot grown in medium added with IAA was found. After 30 days of acclimatization period, the largest survival percentage was obtained from shoots cultivated in medium with 1 mg L-1 of IBA and IAA (88% and 92%, respectively. Although, IBA provided the highest in vitro rooting, most of the surviving shoots were those originated in IAA-added medium, probably because IBA promoted longer fibrous roots, less appropriate for transplant and soil fixation, as they are easily damaged. It was concluded that in vitro rooting with the addition of the highest IAA concentration (1 mg L-1 provided the greatest plant survival during the acclimatization period of the Japanese plum cv. América.

  5. The Rosa chinensis cv. Viridiflora Phyllody Phenotype Is Associated with Misexpression of Flower Organ Identity Genes

    Science.gov (United States)

    Yan, Huijun; Zhang, Hao; Wang, Qigang; Jian, Hongying; Qiu, Xianqin; Baudino, Sylvie; Just, Jeremy; Raymond, Olivier; Gu, Lianfeng; Wang, Jihua; Bendahmane, Mohammed; Tang, Kaixue

    2016-01-01

    Phyllody is a flower abnormality in which leaf-like structures replace flower organs in all whorls. Here, we investigated the origin and the molecular mechanism of phyllody phenotype in Rosa chinensis cv. Viridiflora, an ancient naturally occurring Chinese mutant cultivar. Reciprocal grafting experiments and microscopy analyses, demonstrated that the phyllody phenotype in Viridiflora is not associated with phytoplasmas infection. Transcriptome comparisons by the mean of RNA-Seq identified 672 up-regulated and 666 down-regulated genes in Viridiflora compared to its closely related genotype R. chinensis cv. Old Blush. A fraction of these genes are putative homologs of genes known to be involved in flower initiation and development. We show that in flower whorl 2 of Viridiflora, a down-regulation of the floral organ identity genes RcPISTILLATA (RcPI), RcAPETALA3 (RcAP3) and RcSEPALLATA3 (RcSEP3), together with an up-regulation of the putative homolog of the gene SUPPRESSOR of OVEREXPRESSION of CONSTANS1 (RcSOC1) are likely at the origin of the loss of petal identity and leaf-like structures formation. In whorl 3 of Viridiflora, ectopic expression of RcAPETALA2 (RcAP2) along with the down regulation of RcPI, RcAP3, and RcSEP3 is associated with loss of stamens identity and leaf-like structures formation. In whorl 4, the ectopic expression of RcAP2 associated with a down-regulation of RcSEP3 and of the C-class gene RcAGAMOUS correlate with loss of pistil identity. The latter also suggested the antagonist effect between the A and C class genes in the rose. Together, these data suggest that modified expression of the ABCE flower organ identity genes is associated with the phyllody phenotype in the rose Viridiflora and that these genes are important for normal flower organs development. PMID:27462328

  6. [Models for estimating foliar Fe and Mn Concentration of Armeniaca vulgaris cv. Luntaibaixing using spectral reflectance].

    Science.gov (United States)

    Hu, Zhen-Zhu; Pan, Cun-De; Wang, Shi-Wei; Guo, Zhi-Chao; Wang, Qing-Tao; Ding, Fan; Li, Yuan

    2014-09-01

    Aimed at providing technology for a rapid nutrition diagnosis system of micronutrients in Armeniaca vulgaris cv. Luntaibaixing, we established an element concentration estimation model for its foliar ferrum (Fe) and manganese (Mn) concentration based on spectrum analysis. The foliar spectrum reflectance at various phenological periods of fruit development under different soil fertility conditions was measured by Unispec-SC spectrometer. By analyzing the correlation of foliar Fe, Mn concentration at various phenological periods of fruit development, the spectrum reflectance Rλ and its first-order differential f' (Rλ), we filtered out its sensitive bands. And we established an element concentration estimation model for its foliar Fe and Mn at various phenological periods of fruit development with the linear regression model. The results showed that the spectral sensitive bands of foliar Fe in fruit setting period were 873 and 874 nm, 375 and 437 nm in fruit core-hardening period, 836 and 837 nm in maturity period and 325 and 1 054 nm in post-harvest period. However, the spectral sensitive bands of Mn were 913 and 1 129 nm, 425 and 970 nm, 390 and 466 nm, 423 and 424 nm, respectively. The Fe and Mn concentration of A. vulgaris cv. Luntaibaixing leaves were the most relevant to the first-order differential f' (RD) of its spectrum reflectance, whose linear spectrum estimation model fitting degree was the highest and reached to a significant or highly significant level. It showed that the spectral sensitive bands of Fe and Mn element varied with different phenological periods of fruit development. The spectrum estimation models for its foliar Fe and Mn concentration could be established with linear model according to its first-order differential f' (Rλ). PMID:25532350

  7. Sensory analysis and volatile compounds of olive oil (cv. Cobrancosa) from different irrigation regimes

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes-Silva, A. A.; Falco, V.; Correia, C. M.; Villalobos, F. J.

    2013-05-01

    The aim of this study was to assess the effect of different irrigation strategies on the sensory quality of virgin olive oil VOO) from the cv. cobrancosa- integrated into a protected denomination of origin of Azeite de Tras-os-Montes in the Northeast of Portugal. Three irrigation treatments were applied: (T2)-full irrigation, which received a seasonal water equivalent of 100% of the estimated crop evapotranspiration (ET{sub c}), (T1)-continuous deficit irrigation (30% ETc) and (T0)- rainfed treatment. Data were collected from two consecutive crop years (2005-2006). Olive oil samples were analyzed for volatiles by GC-MS and the results compared with sensory evaluation data. Total volatile compounds tended to decrease with the amount of water applied. The characteristics pungent and bitter were more pronounced in olive oils from T0 and T1, which had higher polyphenolic concentrations, with a strong positive relationship with this variable and the bitter attribute. The Principal Components Analysis clearly separates the three olive oils from 2005, the driest year, and aggregates into a single group the three samples from 2006, suggesting no effect of irrigation on volatile compounds in years with a rainy spring and a marked effect in years with severe drought, suggesting that the effect of the trees’ water status on these variables occurs throughout the crop season and not just during the oil accumulation phase. In general, olive oil from the cv. Cobrançosa is more bitter than pungent and has a typical nutty sensory attribute shown by a strong positive relationship between benzaldehyde and the sensory notes of almonds and nuts. (Author) 34 refs.

  8. Tacit Knowledge Transfer Mechanism on the Face-to-face Communication Platform:A Case Analysis on Cavendish Laboratory%面对面交流平台中的隐性知识传递机制--基于卡文迪什实验室的案例分析

    Institute of Scientific and Technical Information of China (English)

    张桂芳; 吴戈; 周程

    2014-01-01

    To understand the transfer mechanism of tacit knowledge is a precondition for knowledge innovation and management. This passage chooses Cavendish laboratory in the charge of J.J. Thomson as the case and analyses the transfer routes of different kinds of tacit knowledge. It indicates: an effective transfer of tacit knowledge in R&D departments to large degree depends on the openly face-to-face communication platform. The auxiliarily skilled tacit knowledge is integrated and employed by the individual through eternal communication platform, while the critical skilled tacit knowledge like experiment method and the cognitive tacit knowledge like experiment thought are learned and absorbed by the individual through internal communication platform. Compared to the skilled tacit knowledge, the cognitive tacit knowledge has a more far-reaching impact on basic research e ciency. A technological gate keeper firstly ensure to mobilize the resources of skilled tacit knowledge for the research need of scientific communities, secondly and more profoundly shape the research routines and cultural atmosphere of scientific communities. This research is helpful for research institutes to improve performance of knowledge innovation and management.%破解隐性知识的传递机理是寻求知识创新与管理途径的必要前提。本文以J.J.汤姆逊管理时期的卡文迪什实验室为例,分析不同种类的隐性知识在面对面交流平台中的传递路径。研究发现:开放式的面对面交流平台有助于基础研究部门隐性知识的传递。其中,与实验相关的辅助技能型隐性知识主要通过与外界同行的面对面交流被个体整合利用,关于选题和实验方法的核心技能型隐性知识及其背后体现的思维和理念的认知型隐性知识则主要通过师徒之间的面对面交流被个体内化吸收。认知型隐性知识相比技能型隐性知识,对促进知识创新和管理具

  9. 转基因大豆BPS-CV127-9 PCR定量检测研究%Quantitative Detection of Genetically Modified Soybean BPS-CV127-9

    Institute of Scientific and Technical Information of China (English)

    李宁; 赵蕾; 孙红炜; 李凡; 杨淑珂; 路兴波

    2012-01-01

    A quantitative method to detect the transgenic soybean BPS-CV-127-9 by using real-time PCR technique based on fluorescence dye SYBR Green I was investigated in this study. The endogenous lectin gene and the 5' flanking sequences of BPS-CV-127-9 were amplified through the specific primers,and the transgenic content was then calculated according to the standard curve equation. Meanwhile the specificity of PCR amplification was analyzed by corresponding melting curves. The results showed that the standard curves of lectin and the 5' flanking sequences of BPS-CV-127-9 genes have good linear relationship, and their R values were 0. 999 and 0. 998, respectively. The coefficient of variance was 1. 50%-18. 51% and standard deviation was 0.02-0.07. Four mixed samples with genetically modified contents of BPS-CV-127-9 was 0.05% ,0.1% ,0.5% and 1% respectively were detected,and the detection results agreed well with actual value. In conclusion,this method was fast, sensitive, simple, accurate, specific and of high throughput, and could be used to detect transgenic soybean BPS-CV-127-9 quantificationally.%采用SYBR Green实时荧光定量PCR技术,建立转基因大豆BPS-CV127-9的定量检测方法.通过设计特异引物,扩增内标准基因lectin和BPS-CV127-9的5’侧翼序列,建立2种基因的拷贝数-CT标准曲线,根据标准曲线方程计算样品中的转基因含量,并且通过熔解曲线分析扩增反应特异性.结果表明,lectin基因和侧翼序列标准曲线线性关系良好,R2值分别为0.999和0.998,变异系数(CV) 1.50%~18.51%、标准偏差(SD)0.02 ~0.07.检测4个已知BPS-CV127-9含量(1%、0.5%、0.1%、0.05%)的转基因混合样品,实测值与实际值接近.该检测方法具有快速、灵敏、准确、特异、高通量等优点,可以作为转基因大豆BPS-CV127-9的定量检测方法.

  10. Practice of Creating National AAA Safe and Civilized Demonstration Site in North Huaqiang Metro Station%华强北地铁创建国家级AAA安全文明示范工地实践

    Institute of Scientific and Technical Information of China (English)

    徐新; 吴晓斌

    2015-01-01

    创建国家级AAA安全文明示范工地,提高地铁工程建设的形象,打造精品工程,增强企业的竞争力,提高安全意识和管理水平,交流先进管理经验,抓好工程整体推进及成本投入控制。采取“压噪音、抑扬尘、绝泥水、靓围挡”的文明施工总要求,减少对周边居民和商铺产生的影响,严格落实质量安全文明生产各项规定并形成长效机制,为把华强北建设成国家AAA级安全文明标准化诚信工地奠定了坚实的基础。%This paper tries to create national AAA safe and civilized demonstration site, improve the image of subway construction, create fine works, enhance the competitiveness of enterprises, improve safety awareness and management level, exchange advanced management experience, and grasp the overall progress and cost investment control of project. This paper takes the general requirements of civil construction of "noise reduction, dust suppression, never muddy, good enclosure" to reduce the impact on nearby residents and shops, strictly implements the provisions of safety and civilized production quality and forms long-term mechanism, which has laid a solid foundation for the construction of national AAA safe and civilized demonstration site of north Huaqiang metro station.

  11. Regeneração in vitro de melão, cv. 'Gaúcho' In vitro regeneration of melon, cv. 'Gaúcho'

    Directory of Open Access Journals (Sweden)

    Daiane Schmidt de Pinho

    2010-05-01

    Full Text Available O objetivo do presente trabalho foi otimizar um protocolo de regeneração de explantes cotiledonares de melão, cultivar 'Gaúcho', avaliando a composição de meios de germinação e o tempo de permanência dos explantes nesses meios. Para isso, as sementes foram germinadas em meio MS semi-sólido contendo BAP ou ANA e sem esses reguladores de crescimento. As sementes permaneceram nesses meios por um, dois, três e quatro dias, sendo então seus cotilédones inoculados em meio MS contendo diferentes concentrações de BAP (0,5; 0,9; 1,5 e 2,0mg L-1. As maiores taxas de regeneração ocorreram nos cotilédones oriundos de sementes mantidas durante um e dois dias nos meios contendo 0,5 e 0,9mg L-1 de BAP. O aumento da permanência dos explantes nos meios de germinação e as elevadas concentrações de BAP nos meios de regeneração diminuíram a capacidade organogênica dos explantes e incrementaram a formação de calos.The aim of the present study was to optimize a cv. Gaucho melon cotyledon explant regeneration protocol, evaluating the germination media composition and the explant exposition period in these media. For this purpose, seeds were germinated in semi-solid MS medium containing either BAP or ANA, and without growth regulators. The seeds were kept in these media for one, two, three or four days; afterwards, their cotyledons were inoculated in MS medium containing different BAP concentrations (0.5; 0.9; 1.5 and 2.0mg L-1. The highest regeneration rates occurred with cotyledons from seeds which had been kept for one or two days in media containing 0.5 and 0.9mg L-1 BAP. Both the increase in the explant exposition time in germination media and high BAP concentrations in regeneration media decreased explant organogenic capacity, and increased callus formation.

  12. Regeneração de brotações de macieira (Malus domestica, Borkh. cv. Gala Regeneration of apple shoots (Malus domestica, Borkh. cv. Gala

    Directory of Open Access Journals (Sweden)

    MÁRCIA WULFF SCHUCH

    2002-08-01

    Full Text Available Por meio deste trabalho, foi desenvolvido um protocolo de regeneração de brotações em explantes de macieira, cultivar Gala, visando a sua utilização em programas de transformação genética. Para tanto, testaram-se diferentes tipos de explante (folha escarificada, segmento foliar e entrenós, em diferentes meios de cultura, com Benzilaminopurina (BAP e Thidiazuron (TDZ, em concentrações de 0; 3,0; 4,0 e 5,0mg.L-1, e a exposição ou não das brotações, de onde foram retirados os explantes, a uma semana de escuro antes da retirada dos mesmos. As variáveis avaliadas foram percentagem de explantes regenerados, número de brotações por explante e presença de vitrificação. Concluiu-se que a folha escarificada e os segmentos foliares foram os melhores tipos de explantes; a colocação das brotações no escuro durante uma semana aumentou a taxa de regeneração e o número de brotações e diminuiu a necessidade de BAP para formar o mesmo número de brotações do que naqueles mantidos na luz; as melhores concentrações de BAP foram de 4,0 a 5,0mg.L-1 e o TDZ, nas maiores concentrações, causou vitrificação.The main objective of this work was to develop regeneration protocol in order to produce transgenics cv. "Gala" plants. In the regeneration studies, different explant sources were evaluated (wounded leaf; leaf segments; internodes in different culture media containing BAP and TDZ (0; 3,0; 4,0 and 5,0mg.L-1, and the effect of the darkness in the explant source, were also analised in the frequencies and number of regenerate shoots. The wounded leaf and leaf segments shown the best response. Pre-conditioning the explants source during a week in darkness conditions increased the regeneration rates and the number of shoots, decreasing the optimum level of BAP necessary to obtain the same number of shoots. BAP at 4,0 or 5,0mg.L-1 showed better responses however, higher TDZ concentrations promoted vitrification.

  13. AVALIAÇÃO DA CV. CABERNET FRANC PARA ELABORAÇÃO DE VINHO TINTO EVALUATION OF CV. CABERNET FRANC TO ELABORATE RED WINE

    Directory of Open Access Journals (Sweden)

    Luiz Antenor RIZZON

    2001-08-01

    Full Text Available A uva Cabernet Franc, originária da região de Bordeaux, França, foi introduzida no Rio Grande do Sul através da Estação Agronômica de Porto Alegre. É utilizada para a elaboração de vinho tinto para ser consumido jovem, embora apresente aptidão para envelhecer. Face a sua importância, conduziu-se este trabalho com o objetivo de determinar as características agronômicas e enológicas da uva Cabernet Franc para elaboração de vinho tinto. Para isso, realizaram-se estudos para caracterizar o cacho, o mosto e o vinho tinto nas safras de 1987 a 1994. Os resultados obtidos evidenciaram que a cv. Cabernet Franc tem cacho médio, formado por bagas pequenas. O mosto possui teores de açúcar e de acidez adequados para vinificação. O vinho apresenta elevado teor de K e de álcoois superiores. No aspecto sensorial, apresenta cor vermelho-rubi, com reflexos violáceos quando jovem, e de intensidade variável em função das safras vitícolas. No olfato, apresenta aroma com notas vegetais e frutadas, que lembram pimentão e frutas vermelhas, respectivamente. Gustativamente, o vinho apresenta boa estrutura, equilíbrio e personalidade marcante.Cabernet Franc grape is originated from Bordeaux, France. It was introduced in the Serra Gaúcha region in the south of Brazil, by the Estação Agronômica the Porto Alegre, RS. It is used to elaborate young red wine, but it can be aged too. Due to the importance of Cabernet Franc, this work had the objective to determine its agronomic and winemaking characteristics. To achieve this purpose, variables related to the berries, clusters, must, and wine were performed from 1987 to 1994. The results showed that Cabernet Franc grapes have clusters of medium size and small berries. The must has adequate sugar and titratable acidity levels for vinification. The Cabernet Franc wine has a high concentration of K and higher alcohols. The sensory evaluation showed that it has a ruby color with violet reflexes when

  14. Experimental determination of the boundary layer at air-sample inlet positions on the NASA CV 990 aircraft

    Science.gov (United States)

    Bowen, S. W.; Vedder, J. F.; Condon, E. P.

    1984-01-01

    Full-scale, in-flight measurements of the boundary-layer thickness, velocity profile, and flow angle have been made at several sample collection stations on the fuselage of the NASA CV 990. These results are given as functions of Mach number, Reynolds number, yaw, and angle of attack.

  15. Using Multidimensional Rasch Analysis to Validate the Chinese Version of the Motivated Strategies for Learning Questionnaire (MSLQ-CV)

    Science.gov (United States)

    Lee, John Chi-Kin; Zhang, Zhonghua; Yin, Hongbiao

    2010-01-01

    This article used the multidimensional random coefficients multinomial logit model to examine the construct validity and detect the substantial differential item functioning (DIF) of the Chinese version of motivated strategies for learning questionnaire (MSLQ-CV). A total of 1,354 Hong Kong junior high school students were administered the…

  16. Irregularities in the development of male and female gametophytes in the greenhouse carnation (Dianthus caryophpllus L. cv. William Sim.

    Directory of Open Access Journals (Sweden)

    M. A. Zenkteler

    2015-06-01

    Full Text Available It was found that numerous teratological transformations of the pistils and stamens account for the complete or partial female and male sterility in D. caryaphyllus L. cv. William Sim. Moreover, the degeneration of generative organs was accompanied by irregularities in the development of macro-and microsporogenesis.

  17. Extraction of arbutin and its comparative content in branches, leaves, stems, and fruits of Japanese pear Pyrus pyrifolia cv. Kousui.

    Science.gov (United States)

    Sasaki, Chizuru; Ichitani, Masaki; Kunimoto, Ko-Ki; Asada, Chikako; Nakamura, Yoshitoshi

    2014-01-01

    Arbutin is a tyrosinase inhibitor and is extensively used as a human skin-whitening agent. This study investigated the optimum conditions for extracting arbutin by ultrasonic homogenization from discarded branches pruned from Japanese pear (Pyrus pyrifolia cv. Kousui) trees. The arbutin content was measured in the branches and also in the leaves, stems, fruit peel, and fruit flesh.

  18. Chemical Composition, In vivo Digestibility and Metabolizable Energy Values of Caramba (Lolium multiflorum cv. caramba) Fresh, Silage and Hay

    OpenAIRE

    Özelçam, H.; Kırkpınar, F.; TAN, K

    2015-01-01

    The experiment was conducted to determine nutritive values of caramba (Lolium multiflorum cv. caramba) fresh, silage and hay by in vivo and in vitro methods. There was a statistically significant difference (p0.05). However, the highest MECN (ME was estimated using crude nutrients) and MEADF values were found in fresh caramba (p

  19. Exogenous gibberellins inhibit coffee (Coffea arabica cv. Rubi) seed germination and cause cell death in the embryo

    NARCIS (Netherlands)

    Silva, Da E.A.A.; Toorop, P.E.; Nijsse, J.; Bewley, J.D.; Hilhorst, H.W.M.

    2005-01-01

    The mechanism of inhibition of coffee (Coffea arabica cv. Rubi) seed germination by exogenous gibberellins (GAs) and the requirement of germination for endogenous GA were studied. Exogenous GA4+7 inhibited coffee seed germination. The response to GA4+7 showed two sensitivity thresholds: a lower one

  20. Evidence for Oxygen-Isotope Exchange in Chondrules and Refractory Inclusions During Fluid-Rock Interaction on the CV Chondrite Parent Body

    Science.gov (United States)

    Krot, A. N.; Nagashima, K.

    2016-08-01

    Plagioclase in chondrules, CAIs and AOAs from the carbonaceous chondrite Kaba (CV3.1) experienced oxygen-isotope exchange with a metasomatic fluid responsible for the formation of magnetite, fayalite and Ca,Fe-rich silicates on the CV parent body.

  1. High floral bud abscission and lack of open flower abscission in Dendrobium cv. Miss Teen: rapid reduction of ethylene sensitivity in the abscission zone

    NARCIS (Netherlands)

    Bunya-atichart, K.; Ketsa, S.; Doorn, van W.G.

    2006-01-01

    We studied the abscission of floral buds and open flowers in cut Dendrobium inflorescences. Abscission of floral buds was high and sensitive to ethylene in all cultivars studied. Many open flowers abscised in most cultivars, but cv. Willie exhibited only small amount of floral fall and cv. Miss Teen

  2. Chlorophyll Fluorescence in Partially Defoliated Grape Plants (Vitis vinifera L. cv. Chardonnay / Fluorescencia de la Clorofila en Plantas de Uva (Vitis vinifera L. cv. Chardonnay Defoliadas Parcialmente

    Directory of Open Access Journals (Sweden)

    Peña Olmos Jaime Ernesto

    2013-08-01

    Full Text Available The chlorophyll content and fluorescence weredetermined in five-year-old grape plants (Vitis vinifera L. cv.Chardonnay that were subjected to early partial defoliation,in Villa de Leyva, Colombia. The experimental design wascompletely randomized, consisting of two treatments (50%defoliation and control, each with four replications of 35 plants. Every two weeks, one of every two recently-emerged leaves was removed from the non-control plants. The determination of total chlorophyll content was carried out on six leaves per plant using a CCM-200 Plus chlorophyll meter, while chlorophyll fluorescence measurements were taken with one darkadapted leaf per plant using a Junior-PAM fluorometer. Initial fluorescence (Fo, maximum fluorescence (Fm, terminal fluorescence (Ft, variable fluorescence (Fv, electron transport rate (ETR, maximum photochemical quantum yield of PSII (Fv/ Fm, effective photochemical quantum yield of photosystem II (Y(II, photochemical fluorescence quenching coefficient (qP, two non-photochemical quenching coefficients (qN and NPQ,quantum yield of light-induced non-photochemical fluorescence quenching (Y(NPQ, and quantum yield of non-light-induced non-photochemical quenching (Y(NO were measured. The chlorophyll concentration index showed higher values in the defoliated plants. There were no significant differences for the values of Fm, Ft and Fv. Fo was higher in the defoliated plants, while ETR, Fv/Fm and Y(II showed higher values in the control plants. It is evident that a reduction in leaf area modifies thepartitioning of excitation energy destined for photochemicaland non-photochemical processes, thus directly influencing the photosynthetic process of the plants evaluated. / Utilizando un diseño completamente aleatorizado,con dos tratamientos (defoliación al 50% y control y cuatrorepeticiones de 35 plantas cada una, se determinó el contenido y la fluorescencia de la clorofila en plantas de uva, sometidas a defoliación parcial

  3. Produção integrada e convencional de pêssegos cv. Marli Integrated and convetional production of peach cv. Marli

    Directory of Open Access Journals (Sweden)

    José Luis da Silva Nunes

    2004-12-01

    Full Text Available O cultivo de pessegueiros é uma atividade de grande importância econômica no Sul do Brasil, onde se destaca o Estado do Rio Grande do Sul como maior produtor brasileiro. Um dos aspectos mais importantes na produção de alimentos da atualidade é a redução no uso de agroquímicos, com menor contaminação do ambiente e riscos reduzidos de resíduos. Este trabalho visou a comparar os sistemas de Produção Convencional (PC e Integrada (PI de pêssegos e foi realizado no ano de 2001, no município de São Jerônimo - RS, latitude 30°05'52" S, longitude 51°39'08" W e altitude de 46 metros. Áreas de um pomar comercial da cv. Marli foram avaliadas em relação às principais práticas de manejo da planta e do solo, controle fitossanitário, aspectos econômicos, bem como à qualidade da fruta. Na área conduzida sob PI, foram utilizadas as práticas de manejo preconizadas pelas Normas de Produção Integrada de Pêssegos (NPIP e, na área conduzida no sistema de PC, as plantas foram manejadas de acordo com as práticas comumente utilizadas pelo produtor. A produção de pêssegos, em ambos os sistemas, não foi afetada. Na área de PI, houve menor número de pêssegos por planta; entretanto, as frutas apresentaram maior peso médio. A maioria dos pêssegos da PI foram classificados como CAT I (diâmetro superior a 57 mm. As frutas produzidas na PC são, na maioria, de CAT II (de 48 a 57 mm. A qualidade pós-colheita não apresentou diferenças em relação à acidez, firmeza e cor. Com base nestes resultados, podemos concluir que é possível produzir pêssegos de qualidade com produtividade no sistema de PI.The peach-trees cultivation is a very economic important activity in South of Brazil, where highlights the State of Rio Grande do Sul as the largest Brazilian producer. One of the most important aspects in actuality is the food prodution with reduction of agrochemicals components, reducing the risks of environment contamination. This work

  4. 单纱强力CV%值的试验研究%Test Research of Coefficient of Variability on Single End Strength

    Institute of Scientific and Technical Information of China (English)

    薛少林; 周秀玲; 马文祥; 李艳华; 刘向花

    2000-01-01

    以涤棉混纺纱为例,就纱线不匀、锭速和捻度不匀、细纱工艺和混纺比等因素对单纱强力CV%值的影响进行了试验分析,并用数学统计的方法得出条干CV%值、百米重量CV%值、细纱锭速不匀、捻度不匀与成纱单强CV%值正相关.指出合理配置细纱牵伸和加捻卷绕工艺,选择合适的钢丝圈和捻系数是降低成纱单强CV%值的有效措施.

  5. Using Gamma Irradiation To Induce New Mutants In Potatoes Cv. Diamant Through Tissue Culture Technique

    International Nuclear Information System (INIS)

    The excess salt, usually NaCl, inhibits potato plant growth and decreases tubers yield. The use of gamma irradiation to induce new mutants in potato cv. Diamant through tissue culture technique was the main task of this study. Sterilized meristemic tips of potato tubers were cultured on aseptic solid MS-medium, pH 5.7, and were incubated at 20 ± 2deg C and 16 hrs day length of 3000-Lux light intensity, to produce virus-free plantlets. Micro-propagation started after 6-8 weeks and plantlets were sub-cultured every 3-4 weeks to increase plantlets population. Plantlets were exposed to 0, or 40 Gy, dose rate 27.7 rad / sec., using Co60 source at the National Center for Research and Radiation Technology, Cairo, Egypt. Irradiated and unirradiated plantlets were transplanted into 60 ml liquid 1/2MS-medium, pH 5.7, and supplemented with 0, 2000 or 4000 ppm NaCl. And, they were incubated for 2 weeks under the same conditions of temperature and light till the new plantlets were grown up. Healthy plantlets were selected, and micro-propagated up to the sixth vegetative generation (M1V6), under the same conditions of salinity and incubation conditions Thereafter, the plantlets were transferred to tuberization liquid 1/2MS-medium, supplemented with the same mentioned concentrations of NaCl, to obtain microtubers. The microtubers were collected after 6-8 weeks and preserved at 10 deg C for 3 months approximately, to break the dormancy. Sprouted microtubers were sown to obtain minitubers, and subsequently macrotubers. All cultures were performed in 30-cm pots in a protected greenhouse, and were irrigated with the same concentrations of NaCl. It could be elicited that cv. Diamant is salinity sensitive. This was evidenced by the decrease in the average number of tubers per plant and average fresh weight of tuber under salinity stress up 4000 ppm NaCl, comparing to unsaline control treatment. Potato plants, which still healthy and produced tubers under salinity stress up to the 6th

  6. Study on External Application of Eleutherosides Ointment on Shenque (CV 8) for Resisting Sleep Deprivation

    Institute of Scientific and Technical Information of China (English)

    温瑞丽; 王升旭; 李求实; 黄国琪

    2006-01-01

    Objective: To investigate the efficacy of external application of Eleutherosides ointment on Shenque (CV 8) for treating sleep deprivation. Methods: Twenty four healthy young male volunteers at the age of 19-22 years old were randomly allocated to 4 groups (normal, model, treatment and control groups). Sleep was deprived for 48 hours except the normal group. Self-made Eleutherosides compound preparation was applied externally on Shenque (CV 8), to observe its influence on psychology and blood biochemical indices of cortisol and testosterone of the human body in sleep deprivation. Results: After sleep deprivation for 48 hours, an increment in cortisol concentration was lower in the Eleutherosides group (44.482 ± 96.065 nmol/L) than in the control group (146.809 ± 71.075 nmol/L); an increment in the self-evaluated depression scale was also lower in the Eleutherosides group (2.833 ± 16.746) than in the control group (20.417 ± 10.358). There were significant differences (P<0.05). But, there was no significant difference between the treatment group and control group (P>0.05) in the decrement of testosterone concentration and the increment of the anxiety scale. Conclusion: External application of Eleutherosides preparation on Shenque (CV 8) can regulate the stress reaction level and psychological endurance of the human body and produce an effect to resist sleep deprivation.%目的:观察刺五加总甙神阙穴位贴敷疗法的抗睡眠剥夺作用.方法:健康青年男性19~22岁)志愿者24名,完全随机分为4组(正常组、模型组、处理组、对照组),除正常组外剥夺全部睡眠48 h,以自制刺五加总甙复方制剂于神阙穴处贴敷,观察其对睡眠剥夺人体的心理影响以及对皮质醇、睾酮等血液生化指标的影响.结果:48 h睡眠剥夺后,刺五加总甙穴位贴敷皮质醇浓度增高值(44.482±96.065 nmol/L)低于对照组增高值(146.809±71.075 nmol/L),自评抑郁量表增加值(2.833±16.746)

  7. Studies on stem cuttings of kiwi (Actinidia chinensis PL. CV Bruno

    Directory of Open Access Journals (Sweden)

    Elizabeth O. Ono

    2000-01-01

    Full Text Available The work was carried out to study the effects of some auxins and boron in the rooting of kiwi (Actinidia chinensis Planch cv Bruno stem cuttings.. These cuttings were treated on the base individually with H2O; NAA 300 mg.L-1; IBA 300 mg.L-1; NAA 300 mg.L-1 + Boron; IBA 300 mg.L-1 + Boron; NAA 0,5%-talc and IBA 0,5%-talc. After the treatments, the cuttings were placed in styrofoam trays with vermiculite under moist conditions for 120 days. The evaluation of auxin and boric acid effects were made by observing rooted stem cuttings percentage; reducing and total sugar analysis (g/100 g of dry matter; and tryptophan analysis (in µg/100 mg of dry matter. The effects of such treatments were observed during four seasons of the year. The results showed that summer season was the best for rooting. Use of IBA or NAA in the cuttings showed to be unnecessary.O presente trabalho teve como objetivo, estudar o efeito de auxinas sintéticas e do boro, sobre o enraizamento de estacas caulinares de kiwi (Actinidia chinensisPlanch. cv Bruno. As estacas continham dois nós com aproximadamente 10 cm de comprimento, contendo 2 folhas cortadas ao meio. As bases das estacas receberam os seguintes tratamentos: control (H2O; NAA 300 mg.L-1; IBA 300 mg.L-1; NAA 300 mg.L-1 + B; IBA 300 mg.L-1 + B; NAA 0,5%-pó e IBA 0,5%-pó. Após os tratamentos as estacas foram plantadas em bandejas de enraizamento contendo vermiculita pura e colocadas em câmara de nebulização por 120 dias até a coleta das mesmas. Para a avaliação do efeito das auxinas e boro, foram realizadas as seguintes observações: 1. porcentagem de estacas enraizadas; 2. análise de açúcares redutores e açúcares totais (em g/100 g de matéria seca; 3. análise de triptofano (em µg/100 mg de matéria seca. Além disso, foram verificados o efeito dos tratamentos em quatro épocas, que corresponderam às estações do ano (primavera, verão, outono e inverno. Através dos resultados obtidos no processo de

  8. Controle do amadurecimento do kiwi cv. monty com 1-metilciclopropeno The use of 1-methylcyclopropene in ripening control of kiwi cv. monty

    Directory of Open Access Journals (Sweden)

    Julius Blum

    2009-03-01

    Full Text Available O 1-metilciclopropeno (1-MCP é um gás que atua inibindo o sítio de ação do etileno, aumentando o tempo de armazenamento de frutos. O objetivo deste trabalho foi avaliar o efeito deste gás na conservação pós-colheita de kiwi 'Monty', à temperatura ambiente. Ele consistiu na exposição dos frutos a concentrações de 0; 0,5 e 1,0 mg dm-3 de 1-MCP, durante 24 horas, e posterior estocagem a 20 ± 4 ºC, por até 20 dias, com avaliações a cada 4 dias. Avaliaram-se: firmeza, translucidez da polpa, sólidos solúveis (SS, pH, acidez titulável (AT, pectina, % de frutos firmes para transporte e consumo. A translucidez da polpa mostrou-se um parâmetro adequado para monitoramento do amadurecimento, visto que houve correlação com a firmeza. A aplicação de 1-MCP preservou a firmeza e preveniu a translucidez da polpa, prolongando a qualidade para transporte e consumo por 4 e 12 dias, respectivamente, e não teve efeito sobre o pH, SS e pectinas. Nos tratamentos com 1-MCP, ocorreu um aumento inicial na acidez, mas com o passar do tempo, ocorreu diminuição da mesma, da firmeza e aumento dos sólidos solúveis e da translucidez da polpa, caracterizando o amadurecimento dos frutos.The gas 1-methylcyclopropene acts inhibiting the action site of ethylene, increasing the postharvest period of the fruits. The aim of this work was to evaluate the effect of this gas on postharvest conservation of kiwifruit cv. Monty at ambient temperature. The study consisted of fruit exposition to 1MCP at the concentrations of 0, 0,5 and 1,0 mg dm-3 during 24 hours, and stored at 20 ± 4 ºC per 20 days, with evaluations at 4 days intervals. It had been evaluated: firmness of pulp, pulp color, soluble solids, pH, total acidity, % of pectin and % of firm fruits for transport and consumption. The color of pulp was an adequate parameter to evaluate the ripening of fruits, since it had correlation with the firmness. The application of 1MCP preserved the firmness the

  9. Manutenção da qualidade de rosas cortadas cv. Vega em soluções conservantes Keeping quality of cut roses cv. Vega in holding solutions

    Directory of Open Access Journals (Sweden)

    Júlia de Pietro

    2012-03-01

    Full Text Available A rosa é considerada uma das flores de corte mais tradicionais e requisitadas no mercado mundial. Entretanto, após o corte, essas flores tornam-se altamente perecíveis devido à elevada atividade metabólica que conduz aos processos de senescência. Assim, o experimento foi realizado para verificar o efeito do uso de soluções conservantes na fisiologia pós-colheita de rosas vermelhas cortadas cultivar Vega. Utilizou-se o delineamento inteiramente casualizado, em esquema fatorial composto por dois fatores: sete tratamentos pós-colheita e quatro datas de avaliação. As flores permaneceram nas seguintes soluções de manutenção: 1 Água destilada; 2 8-hidroxiquinolina (200 mg L-1; 3 8-hidroxiquinolina (200 mg L-1 + Sacarose (20 mg L-1; 4 Ácido cítrico (75 mg L-1; 5 Ácido cítrico (75 mg L-1 + Sacarose (20 mg L-1; 6 6-benziladenina (60 mg L-1; 7 6 benziladenina (60 mg L-1 + Sacarose (20 mg L-1. Foram realizadas análises qualitativas e fisiológicas ao longo do período de avaliação. O tratamento com 8-hidroxiquinolina e ácido cítrico revelaram-se mais promissores para manter a qualidade e prolongar a vida de vaso das flores, o que foi evidenciado pela menor perda de massa fresca, atividade respiratória e conteúdo relativo de água; melhor manutenção do conteúdo de carboidratos solúveis e redutores, antocianina e coloração.The rose is considered one of the most traditional and required cut flowers in the world market. However, after cutting, these flowers become highly perishable, because the high metabolic activity leads to senescence processes. The experiment was accomplished to verify the effect of holding solutions on the postharvest physiology of cut red roses, cv. Vega. A randomized design in a factorial scheme composed by two factors was used: seven postharvest treatments and four evaluation dates. The flowers remained in the following holding solutions: 1 Distilled water; 2 8-hydroxyquinoline citrate (200 mg L-1; 3 8

  10. Qualidade da maçã cv. Gala tratada com 1-metilciclopropeno Quality of apples cv. Gala treated with 1-methylcyclopropene

    Directory of Open Access Journals (Sweden)

    Auri Brackmann

    2004-10-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência da aplicação inicial de 1-metilciclopropeno (1-MCP e o efeito de diferentes doses na reaplicação após quatro meses de armazenamento refrigerado, na manutenção da qualidade de maçãs cultivar Gala. O delineamento experimental utilizado foi o inteiramente casualizado com quatro repetições e unidade experimental composta por 25 frutos. Os tratamentos originaram-se da combinação entre o momento da aplicação (início do armazenamento e após 4 meses de armazenamento e doses de 1-MCP (0, 312, 625nLL-1. Os tratamentos de 1-MCP foram: 0+0nLL-1; 0+312nLL-1; 0+625nLL-1; 625+0nLL-1; 625+312nLL-1 e 625+625nLL-1. Após 180 dias de armazenamento a 0,5ºC mais 14 dias a 20ºC, a aplicação inicial de 625nLL-1 de 1-MCP proporcionou frutos com maior firmeza de polpa e acidez titulável e manteve os frutos mais verdes, porém não apresentando efeito sobre a ocorrência de podridões e sólidos solúveis totais. A reaplicação de 1-MCP, aos quatro meses de armazenamento, não apresentou efeito na manutenção da qualidade dos frutos. Após 14 dias de exposição dos frutos a 20ºC, o 1-MCP, aplicado no início do armazenamento, propiciou maior firmeza de polpa, manteve os frutos mais verdes, com maiores níveis de sólidos solúveis totais, acidez titulável e menor ocorrência de podridões, independente da reaplicação do produto.The objective of this work was to verify the efficacy of the initial application of 1-methylcyclopropene (1-MCP and the effect of different reapplication dosis after 4 months of cold storage on quality of apple cv. Gala. The treatments originated from the combination between application time (at storage and after four months of storage and dosis of 1-MCP (0, 312, 625nLL-1. The treatments were: 0+0nLL-1; 0+312nLL-1; 0+625nLL-1; 625+0nLL-1; 625+312nLL-1 and 625+625nLL-1. After 180 days of storage at 0.5ºC and 14 days at 20ºC, the initial application of 625nLL-1 of 1

  11. IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea

    Directory of Open Access Journals (Sweden)

    Alexandre Bosco de Oliveira

    2010-05-01

    Full Text Available

    This research aimed to develop a protocol for the micropropagation of Mussaenda erytrophylla cv. Rosea. Calcium hypochlorite or sodium hypochlorite (2.5% was used for the sterilization of bracts segments, with or without previous immersion in alcohol 70%. For callus induction, bracts segments were inoculated on MS medium with different concentrations of BAP and NAA. For buds induction, callus were inoculated on MS medium with different concentrations of cytokinins BAP, 2iP, and KIN (0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1. For multiplication, nodal segments from in vitro plants were inoculated in MS medium with BAP, KIN, and 2iP, in the concentrations of 0.0 mg L-1, 0.5 mg L-1, 1.0 mg L-1, and 2.0 mg L-1. For rooting, nodal segments from in vitro plants were inoculated using two MS medium dilutions: full strength and half strength of MS basal salt mixture (macronutrients salts and different concentrations of IBA (0.00 mg L-1, 0.25 mg L-1, 0.50 mg L-1, and 1.00 mg L-1. The best sterilization treatment was achieved by using calcium hypochlorite combined with immersion in alcohol. For callus induction, the MS medium with 0.4 mg L-1 of NAA plus 4.0 mg L-1 of BAP was more efficient. Bud multiplication was improved in MS medium with 0.5 mg L-1 of 2iP or 1.0 mg L-1 of KIN. The highest percentage of explants with roots without emission of callus was observed in the medium with 50% and 100% of MS salts without IBA.

  12. (C-V) and y-parameters determination of JFETs under different environmental conditions

    Energy Technology Data Exchange (ETDEWEB)

    El-Ghanam, S.M., E-mail: safaaghanam@yahoo.co [Women' s College for Art, Science and Education, Ain-Shams University, Heliopolis, Cairo (Egypt)

    2010-10-01

    The C-V characteristics of n-channel JFET have been measured under different environmental conditions of temperature up to 140 deg. C and {gamma}-rays up to 100 kGy. For low bias voltage and frequency, the input capacitance, C{sub iss}, is shown to be a direct function of temperature. On the other hand, its value was shown to decrease from 11.68 down to 8.17 nF due to {gamma}-exposure up to 100 kGy. The y-parameters of common source amplifier were calculated under the influence of temperature and {gamma}-rays. The results show that the susceptance component of the admittance increases due to increase in temperature, while decreasing after {gamma}-exposure. Considering the cutoff frequency f{sub T0}, it is clear that as the temperature increases from 30 up to 140 deg. C, f{sub T0} dropped from 47 MHz down to 5 MHz, measured at 0.8 V. On the other hand its value was shown to increase from 43 MHz up to 102 MHz, measured at the same bias voltage, due to {gamma}-exposure up to 100 kGy.

  13. PROGRAM APLIKASI OPTIMALISASI PERENCANAAN PRODUKSI DENGAN METODE GOAL PROGRAMMING (KASUS CV. G

    Directory of Open Access Journals (Sweden)

    Zahedi

    2010-05-01

    Full Text Available Indonesia today is still a State agrarian. Thus agriculture in Indonesia always get more attention by the government. Many government programs are rolled out to support the agricultural development of Indonesia, and one of them is the fertilizer subsidy to farmers Indonesia. Fertilizer itself is very useful to nourish the soil and provide vitamins to the plants, thus increasing the amount of production generated. Therefore it is necessary fertilizer for agriculture in Indonesia. CV. G company is one of the companies that produce fertilizer. Fertilizer produced by this company is an organic fertilizer and inorganic fertilizer. Problems faced by these companies are in planning the production of fertilizer produced. Companies want to optimize the amount of production of each fertilizer, maximize revenues, minimize production costs, and simultaneously optimize the working hours of employees. To meet these objectives, this research created program package using goal programming methods. Goal programming method can provide an effective solution / equilibrium toward the goals set, and the calculation will be assisted by using the Simplex method that has been modified. This method is an extension of linear programming, linear programming which can only solve problems with a single goal while the goal programming to solve problems with multiple objectives.

  14. Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.

    Science.gov (United States)

    Kanamoto, Hirosuke; Yamashita, Atsushi; Asao, Hiroshi; Okumura, Satoru; Takase, Hisabumi; Hattori, Masahira; Yokota, Akiho; Tomizawa, Ken-Ichi

    2006-04-01

    Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to approximately 36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

  15. Identification of a novel drought tolerance gene in Gossypium hirsutum L. cv KC3

    Directory of Open Access Journals (Sweden)

    Jagadeesh N. Selvam

    2009-02-01

    Full Text Available Development of drought resistant cotton cultivars has long been a major breeding objective, since water stress limits fiber production and productivity. Recent advances in functional genomics can increase the efficiency of conventional breeding for genetic improvement of crop plants for improved abiotic stress resistance. To this end, a novel drought resistance gene was identified by comparing the gene expression profile of Gossypium hirsutum cv KC3 and MCU12 by employing a cDNA-RAPD approach. Physiological and biochemical studies showed that KC3 has relatively better drought tolerance than MCU12. Among 25 random primers, OPA15 has identified differentially expressed cDNA in KC3. Results of BLASTP algorithm have shown that this cDNA has significant homology with P-Glycoprotein, ACC oxidase2 and ABC transporter which are involved either directly, or indirectly, in stress tolerance in animals and plants. Hence, the cDNA sequence identified in this study may be a novel gene that confers drought resistance in cotton as KC3 is well adapted and is widely cultivated in rain-fed tracts of Tamil Nadu, India. Further characterization of this gene may show it has potential application for development of cotton with improved drought resistance, through genetic engineering and/or marker aided selection (MAS

  16. FAKTOR-FAKTOR KEPUASAN PELANGGAN DAN LOYALITAS PELANGGAN: Studi Kasus Pada CV. Sarana Media Advertising Surabaya

    Directory of Open Access Journals (Sweden)

    Trisno Musanto

    2004-01-01

    Full Text Available This reseach briefly explains to what extent customer satisfaction has significant influence to the customer loyalty of 'Iklan Jitu' Jawa Pos newspaper in Sarana Media Advertising. Moreover, it also explores variable that give the biggest contribution to the customer loyalty. Four variables are using as customer satisfaction representatives, which are reliability, response to and remedy of problems, sales experience and convenience of acquisition. Finally, the findings shows that sales experience is the biggest contributor to the customer loyalties. Abstract in Bahasa Indonesia : Penelitian ini dimaksudkan untuk mengetahui sejauh mana kepuasan pelanggan mempunyai pengaruh signifikan terhadap loyalitas pelanggan Iklan Jitu surat kabar Jawa Pos pada CV.Sarana Media Advertiseng dan variabel mana yang memberikan kontribusi terbesar pengaruhnya terhadap loyalitas pelanggan. Dalam penelitian ini mengunakan empat variabel untuk mewakili keppuasan pelanggan yang meliputi : reliability,response to and remedy of problems, sales experience dan convenience of acquisition. Dari hasil perhitungan diketahui bahwa sales experience memberikan kontribusi terbesar terhadap loyalitas pelanggan. Kata kunci : kepuasan pelanggan, loyaliras pelanggan.

  17. Investigation of drought stress in pepino (solanum muricatum ait. cv. miskl) leaves

    International Nuclear Information System (INIS)

    In this study, the effects of drought stress on pepino seedlings (Solanum muricatum cv. Miski) under natural greenhouse conditions were examined. The control plants were watered at field capacity, and the stress group was not watered. Samples were collected on the 6th, 12th, 24th and 36th days from the leaves of S. muricatum seedlings that, were exposed to drought stress. The relative water content, the total phenolic compounds, and the malondialdehyde, total photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids), and proline contents in these samples were determined. Depending on drought stress, the relative water content, the chlorophyll a, chlorophyll b and carotenoid contents, and the total chlorophylls were found to be lower in the stress group compared with the control group. In contrast, the total phenolic compounds (24th and 36th days) and the proline (12th, 24th and 36th days) levels increased significantly compared with the control group. In addition, a significant increase in the malondialdehyde contents was obtained on the 36th day in the stress group compared with the control group. Such studies may be important for evaluation of metabolic changes in pepino under the drought stress. (author)

  18. PERDAS QUALITATIVAS DE MAÇÃS CV. GALA EM ARMAZENAMENTO REFRIGERADO

    Directory of Open Access Journals (Sweden)

    BENDER RENAR JOÃO

    2001-01-01

    Full Text Available O presente trabalho foi desenvolvido com o objetivo de dimensionar as perdas de qualidade de maçãs cv. Gala durante o armazenamento refrigerado. Para determinar estas perdas, foi feito um acompanhamento durante três anos consecutivos, com frutos de três tamanhos: calibre em torno de 100 frutos/caixa de 18kg, calibre 130 e calibre acima de 200. Quinzenalmente, durante os primeiros 90 dias de armazenagem no primeiro ano, 144 dias no segundo ano e 211 dias no terceiro ano, amostras de 20 a 30 maçãs foram avaliadas para perda de peso e parâmetros qualitativos como a firmeza de polpa, os sólidos solúveis totais (SST e acidez titulável. As variáveis que apresentaram as melhores correlações com o período de armazenagem e, portanto, podem auxiliar na estimativa de possíveis perdas quantitativas durante o armazenamento em ar-refrigerado foram a firmeza de polpa e a acidez titulável. As maiores perdas de qualidade, com exceção da firmeza de polpa, foram observadas em frutos dos tamanhos menores.

  19. Effect of preservative agents on the respiration rate of minimally processed potato (Solanum tuberosum cv. Monalisa).

    Science.gov (United States)

    Petri, E; Arroqui, C; Angós, I; Vírseda, P

    2008-04-01

    The shelf life of minimally processed potatoes (MPP) is limited by enzyme-catalyzed browning reactions, with the increase in respiration being another factor that affects quality retention of this product. Sulfites are commonly used as effective preservative agents in minimally processing potatoes, but ascorbic acid and citric acid are considered natural sulfite substitutes and more accepted by consumers. The aim of this study was to study the effect of combinations of the preservative agents cited above (sodium metabisulfite 0.1% and 0.5%; citric acid 0.1% and 0.5%; ascorbic acid 0.5%) on the respiration rate of MPP (cv. Monalisa) processed at both ambient and refrigerated temperatures. The results have revealed that there is a significant effect of dipping treatment and temperature on respiration rate of MPP. Sodium metabisulfite (SM) reduces respiratory activity up to 0.8 mL/kg/h. The addition of either citric or ascorbic acid enhanced the effect of SM on the reduction of the respiration rate of MPP. The strongest effect (up to 3.3 mL/kg/h) was observed when a combination of all 3 agents at the higher concentrations was employed at a temperature of 18 degrees C.

  20. Effect of different growing substrates on the yield and cut flower quality of rose cv. Maroussia

    Directory of Open Access Journals (Sweden)

    A. Rezaee

    2013-04-01

    Full Text Available One of the main factors of an aggregate hydroponic system is to use a suitable plant media with local origin. Thus the objective of this research was to study the possibility of replacement of cocopeat as an imported material with local materials and the effects of such media on the growth and flowering characteristics of Rosa hybrida cv. Maroussia. A randomized complete blocks design experiment with four replications was conducted. The treatments were nine growing media: cocopeat (100%, perlite (100%, leca (100%, cocopeat (75% + zeolite (25%, perlite (75% + zeolite (25%, leca (75% + zeolite (25%, cocopeat (50% + perlite (50%, cocopeat (50% + leca (50% and perlite (50% + leca (50%. Results indicated that plants grown in cocopeat and cocopeat + zeolite showed high vegetative and reproductive growth. The highest number of flowers per plant was observed in in cocopeat (8.31 and cocopeat + perlite (7.81 substrates, which had no significant difference with cocopeat + zeolite medium (6.87. Adding zeolite to cocopeat, perlite and leca improved length of flower bud and number of flowers per plant over their pure medium. Also, adding cocopeat to perlite increased fresh and dry weight of cut flowers and number of flowers per plant. Based on the results and availability of zeolite in Iran, mixture of cocopeat (75% + zeolite (25% is recommended.

  1. Sink to Source Transition in Tendrils of a Semileafless Mutant, Pisum sativum cv Curly.

    Science.gov (United States)

    Côté, R; Gerrath, J M; Peterson, C A; Grodzinski, B

    1992-12-01

    Sink to source transition parallels loss of thigmotropic capacity in tendrils of a semileafless mutant, Pisum sativum cv Curly. Macroscopic tendril development is subdivided based on thigmotropic capacity. Stage I is the elongation stage and, although the rate of photosynthesis is similar to that of stage II and III tendrils, dark respiration rates are higher in stage I. During stage II, tendrils are thigmotropic and act as a sink. Even though stage II tendrils have CO(2) exchange characteristics similar to those of stage III tendrils, which are coiled, our fluorescein, (14)C-partitioning, and (11)C-translocation experiments suggest that stage I and II tendrils do not export carbon. Only stage III tendrils act as sources of newly fixed carbon. Export from them is blocked by cold, heat girdling of the petiole, or anoxia treatment of the tendrils. A late stage II tendril complex, in which coiling is occurring, may be exporting photoassimilates; however, this phenomenon can be attributed to the fact that the pea leaf is a compound structure and there may be one or more stage III tendrils, no longer thigmotropic, within the tendril complex. Photosynthetic maturity in pea tendrils occurs at stage III and is characterized by the ability of these tendrils to export photoassimilates. PMID:16653179

  2. Resequencing of Curcuma longa L. cv. Kedaram through transcriptome profiling reveals various novel transcripts.

    Science.gov (United States)

    Sahoo, Ambika; Jena, Sudipta; Sahoo, Suprava; Nayak, Sanghamitra; Kar, Basudeba

    2016-09-01

    Curcuma longa L. (Turmeric), of the family Zingiberaceae, is one of the economically as well as medicinally important plant species. It is a sterile, polyploid and vegetatively propagated spice crop cultivated usually in Southeast Asia. In the current study, we carried out re-sequencing through transcriptome profiling of Curcuma longa cv. Kedaram (Cl_Ked_6). We acquired a total of 1 GB raw data by resequencing through paired-end sequencing using Nextseq 500 platform. The raw data obtained in this study can be accessible in NCBI database with accession number of SRR3928562 with bioproject accession number PRJNA324755. Cufflinks-2.2.1 tool was used for transcriptome assembly which resulted in 39,554 numbers of transcripts. The transcript length ranged from 76 to 15,568, having N50 value of 1221 and median transcript length of 860. We annotated the transcripts using multiple databases. This data will be beneficial for studying sequence variations particularly SNPs between cultivars of turmeric towards authentic identification and discovery of novel functional transcripts in Kedaram. PMID:27595066

  3. Effects of postharvest UV-C treatments on quality and cold storage of cv. Giant plum

    International Nuclear Information System (INIS)

    The aim of this study is to determine effectiveness of different UV-C treatments on fruit quality and cold storage period of cv. Giant plum. After fruits were treated UV-C from 50 and 100 cm distance for 5, 10 and 20 min, they were placed in container (750 g) and packed with polyethylene bag. All packages were stored at 0-10° C and 90±5 % relative humidity for 5 weeks. During the storage period, weight loss, fruit firmness, soluble solids content, titretable acidity, soluble solids content / titretable acidity , fruit juice pH and sensorial (taste and appearance) analyze measurements in fruit samples were performed at 1 week interval. Although it changed according to treatments, weight loss and soluble solids content generally increased; fruit firmness and titretable acidity decreased in the course of storage period. In the research, the highest quality losses were observed in UV-C treatment from 100 cm distance for 5, 20 min and control at the end of 35 days, the best results were obtained from UV-C treatment from 50 cm for 5 and 10 min

  4. Quebra de dormência de gemas de macieira cv. Eva tratadas com cianamida hidrogenada

    Directory of Open Access Journals (Sweden)

    CRUZ JÚNIOR ÁLVARO DE OLIVEIRA

    2002-01-01

    Full Text Available A maioria das cultivares de macieiras plantadas no Brasil apresentam deficiência e desuniformidade de brotação, devido à má distribuição e/ou baixo número de horas de frio abaixo de 7,2masculine C, constituindo-se num entrave à produtividade. Mesmo utilizando-se de cultivares de baixa exigência de frio invernal, como a cultivar Eva, tem-se verificado a necessidade de se fazer a quebra de dormência para uniformizar a brotação, compatibilizar o florescimento e antecipar e melhorar a produção em regiões com invernos amenos ou anos de baixo acúmulo de frio. Avaliou-se o efeito da aplicação de cianamida hidrogenada em três diferentes concentrações (0,5; 1,0 e 1,5 % associada ao óleo mineral (3% na brotação de gemas laterais e terminais de macieiras da cv. Eva, e a fenologia das cvs. Eva e Anabela. Concluiu-se que a concentração de cianamida hidrogenada influi na percentagem de gemas laterais e terminais brotadas, sendo a concentração de 1,5% de cianamida hidrogenada associada a 3% de óleo mineral a mais efetiva na quebra da dormência das gemas

  5. EFFICIENCY OF RAW MATERIAL INVENTORIES IN IMPROVING SUPPLY CHAIN PERFORMANCE of CV. FIVA FOOD

    Directory of Open Access Journals (Sweden)

    Artadi Nugraha

    2016-01-01

    Full Text Available The production and number of processed food industries have slightly increased; as a result, the companies must compete to maximize their profits by conducting their efficient production process. CV. Fiva Food is one of the companies in the field of processed foods, especially in processed meat that has implemented supply chain management. It is necessary for the company to take measurements of its performance and efficiency for the entire supply chain such as procurement of raw materials. The purposes of this study were to analyze the performance of the company's supply chain and determine the most efficient  method of procurement for its raw materials as well as and to provide recommendations for the company to improve its performance of entire supply chain. This study used SCOR in analyzing the performance of supply chain and EOQ and POQ method to be compared with the method that the company uses to determine which method of procurement for raw materials is the most efficient one. The result showed that based on the matrix, the company's performance is unfavorable when it was compared to the benchmark performance of inventory days of supply. In addition, this study showed that the POQ method produces the lowest total inventory cost with savings of Rp6.647.015 for raw materials of MDM whereas EOQ method produced the lowest total inventory cost with savings of Rp222.153,78 for raw materials of FQ85CL. Keywords: performance suppy chain, SCOR, fiva food, EOQ, POQ

  6. The BANANA project. V. Misaligned and precessing stellar rotation axes in CV Velorum

    CERN Document Server

    Albrecht, Simon; Torres, Guillermo; Fabrycky, Daniel C; Setiawan, Johny; Gillon, Michael; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

    2014-01-01

    As part of the BANANA project (Binaries Are Not Always Neatly Aligned), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of $\\beta_{\\rm p} = -52\\pm6^{\\circ}$ and $\\beta_{\\rm s}= 3\\pm7^{\\circ}$ for the primary and secondary stars (B2.5V $+$ B2.5V, $P=6.9$ d). We combine this information with several measurements of changing projected stellar rotation speeds ($v \\sin i_{\\star}$) over the last $30$ years, leading to a model in which the primary star's obliquity is $\\approx65^{\\circ}$, and its spin axis precesses around the total angular momentum vector with a period of about $140$ years. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the $v \\sin i_{\\star}$. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronge...

  7. Air-sampling inlet contamination by aircraft emissions on the NASA CV-990 aircraft

    Science.gov (United States)

    Condon, E. P.; Vedder, J. F.

    1984-01-01

    Results of an experimental investigation of the contamination of air sampling inlets by aircraft emissions from the NASA CV-990 research aircraft are presented. This four-engine jet aircraft is a NASA facility used for many different atmospheric and meteorological experiments, as well as for developing spacecraft instrumentation for remote measurements. Our investigations were performed to provide information on which to base the selection of sampling locations for a series of multi-instrument missions for measuring tropospheric trace gases. The major source of contamination is the exhaust from the jet engines, which generate many of the same gases that are of interest in atmospheric chemistry, as well as other gases that may interfere with sampling measurements. The engine exhaust contains these gases in mixing ratios many orders of magnitude greater than those that occur in the clean atmosphere which the missions seek to quantify. Pressurized samples of air were collected simultaneously from a scoop located forward of the engines to represent clean air and from other multiport scoops at various aft positions on the aircraft. The air samples were analyzed in the laboratory by gas chromatography for carbon monoxide, an abundant combustion by-product. Data are presented for various scoop locations under various flight conditions.

  8. Hidden secrets of deformation: Impact-induced compaction within a CV chondrite

    Science.gov (United States)

    Forman, L. V.; Bland, P. A.; Timms, N. E.; Collins, G. S.; Davison, T. M.; Ciesla, F. J.; Benedix, G. K.; Daly, L.; Trimby, P. W.; Yang, L.; Ringer, S. P.

    2016-10-01

    The CV3 Allende is one of the most extensively studied meteorites in worldwide collections. It is currently classified as S1-essentially unshocked-using the classification scheme of Stöffler et al. (1991), however recent modelling suggests the low porosity observed in Allende indicates the body should have undergone compaction-related deformation. In this study, we detail previously undetected evidence of impact through use of Electron Backscatter Diffraction mapping to identify deformation microstructures in chondrules, AOAs and matrix grains. Our results demonstrate that forsterite-rich chondrules commonly preserve crystal-plastic microstructures (particularly at their margins); that low-angle boundaries in deformed matrix grains of olivine have a preferred orientation; and that disparities in deformation occur between chondrules, surrounding and non-adjacent matrix grains. We find heterogeneous compaction effects present throughout the matrix, consistent with a highly porous initial material. Given the spatial distribution of these crystal-plastic deformation microstructures, we suggest that this is evidence that Allende has undergone impact-induced compaction from an initially heterogeneous and porous parent body. We suggest that current shock classifications (Stöffler et al., 1991) relying upon data from chondrule interiors do not constrain the complete shock history of a sample.

  9. CV-990 Landing Systems Research Aircraft (LSRA) flight #145 drilling of shuttle tire using Tire Assa

    Science.gov (United States)

    1995-01-01

    Created from a 1/16th model of a German World War II tank, the TAV (Tire Assault Vehicle) was an important safety feature for the Convair 990 Landing System Research Aircraft, which tested space shuttle tires. It was imperative to know the extreme conditions the shuttle tires could tolerate at landing without putting the shuttle and its crew at risk. In addition, the CV990 was able to land repeatedly to test the tires. The TAV was built from a kit and modified into a radio controlled, video-equipped machine to drill holes in aircraft test tires that were in imminent danger of exploding because of one or more conditions: high air pressure, high temperatures, and cord wear. An exploding test tire releases energy equivalent to two and one-half sticks of dynamite and can cause severe injuries to anyone within 50 ft. of the explosion, as well as ear injury - possibly permanent hearing loss - to anyone within 100 ft. The degree of danger is also determined by the temperature pressure and cord wear of a test tire. The TAV was developed by David Carrott, a PRC employee under contract to NASA.

  10. Optimalizace procesorového jádra pro knihovnu OpenCV

    OpenAIRE

    Benna, Filip

    2015-01-01

    Tato bakalářská práce se zabývá překladem knihovny OpenCV pro procesorové jádro Codix RISC a následnou optimalizací vybraných aplikací implementovaných s použitím této knihovny. Optimalizace je založena na rozšiřitelnosti procesorového jádra, proto je v této práci teoreticky popsáno a na příkladu vysvětleno přidání vektorových instrukcí do instrukční sady procesoru. Nakonec jsou uvedeny dosažené výsledky a jejich porovnání s neoptimalizovanou verzí aplikace. This bachelor's thesis deals wi...

  11. Agronomic evaluation of coffee tree “Mundo Novo” cv. in Minas Gerais State

    Directory of Open Access Journals (Sweden)

    Bruno de Souza Monte Raso

    2015-08-01

    Full Text Available Aiming of selecting generations of Arabic coffee plants adapted to different coffee regions of the state of Minas Gerais, experiments were conducted in the cities of Três Pontas, Campos Altos and Capelinha. Thirty three progenies from the “Mundo Novo” cv. were evaluated obtained by the genetic improving program of the coffee plants led by the Instituto Agronomico in Campinas. The experiments were carried out in randomized complete block design with four repetitions and six plants by parcel. The yield analysis were performed conjoint for the three sites and six crops. The adaptability and stability of the individual features were evaluated applying the methodology proposed by Annicchiarico (1992, estimating the Confidence Index (Ii and defining as environment the combination between each biennal and each place, that is, nine environments. The parameters fruit maturation stage, floating grains bean/ fruit and bean size were carried out considering the medium of the last two crops, in Três Pontas. The most promising are the IAC 2931, IAC 379-19, IAC 480, IAC 388-6-16 and IAC 379-19-2 because they showed higher stability in the environments and were among the most productive ones in the average of the nine environments, obtaining higher confidence indexes. The progenies IAC 515-8, IAC 501 12, IAC 464 15 have the best percentages of fruit maturation stage, floating grains bean/fruit and bean size.

  12. [In vitro and in vivo effects of mango pulp (Mangifera indica cv. Azucar) in colon carcinogenesis].

    Science.gov (United States)

    Corrales-Bernal, Andrea; Amparo Urango, Luz; Rojano, Benjamín; Maldonado, Maria Elena

    2014-03-01

    Mango pulp contains ascorbic acid, carotenoids, polyphenols, terpenoids and fiber which are healthy and could protect against colon cancer. The aim of this study was to evaluate the antiproliferative and preventive capacity of an aqueous extract of Mangifera indica cv. Azúcar on a human colon adenocarcinoma cell line (SW480) and in a rodent model of colorectal cancer, respectively. The content of total phenolics, flavonoids and carotenoids were also analyzed in the extract. SW480 cell growth was inhibited in a dose and time dependent manner by 22.3% after a 72h exposure to the extract (200 µg/ mL). Colon carcinogenesis was initiated in Balb/c mice by two intra-peritoneal injections of azoxymethane (AOM) at the third and fourth week of giving mango in drinking water (0.3%, 0.6%, 1.25%). After 10 weeks of treatment, in the colon of mice receiving 0.3% mango, aberrant crypt foci formation was inhibited more than 60% (p=0,05) and the inhibition was dose-dependent when compared with controls receiving water. These results show that mango pulp, a natural food, non toxic, part of human being diet, contains bioactive compounds able to reduce growth of tumor cells and to prevent the appearance of precancerous lesions in colon during carcinogenesis initiation. PMID:25796713

  13. SDSS J0018+3454: An Extremely Long Orbital Period CV

    CERN Document Server

    Gonzalez, D; Zharikov, S; Aviles, A; Echevarria, J

    2010-01-01

    We present here results of an optical spectroscopic study of a new Cataclysmic Variable SDSS J001856.93+345444.3. We demonstrate that the most probable value of the orbital period of the system is Porb = 0.6051 \\pm 0.022 days (=14.5226 hours), based on the measurements of radial velocity of a complex of absorption features emanating from the K2-K4V type secondary component. However, the radial velocity measurements from the emission lines are best folded with the period Pem = 0.5743day (=13.78 hours). The gamma-velocity of the emission lines varies significantly from epoch to epoch. There is an underlying broader and weaker component to the emission lines, which we could not resolve. Based on the appearance of the emission lines, the presence of very strong He II lines and the moderate polarization detected by Dillon et al. (2008), we conclude that SDSS J0018+3454 is an asynchronous magnetic CV (Polar).

  14. Composition of cuticular waxes coating flag leaf blades and peduncles of Triticum aestivum cv. Bethlehem.

    Science.gov (United States)

    Racovita, Radu C; Hen-Avivi, Shelly; Fernandez-Moreno, Josefina-Patricia; Granell, Antonio; Aharoni, Asaph; Jetter, Reinhard

    2016-10-01

    The work herein presents comprehensive analyses of the cuticular wax mixtures covering the flag leaf blade and peduncle of bread wheat (Triticum aestivum) cv. Bethlehem. Overall, Gas Chromatography-Mass Spectrometry and Flame Ionization Detection revealed a wax coverage of flag leaf blades (16 μg/cm(2)) a third that of peduncles (49 μg/cm(2)). Flag leaf blade wax was dominated by 1-alkanols, while peduncle wax contained primarily β-diketone and hydroxy-β-diketones, thus suggesting differential regulation of the acyl reduction and β-diketone biosynthetic pathways in the two analyzed organs. The characteristic chain length distributions of the various wax compound classes are discussed in light of their individual biosynthetic pathways and biosynthetic relationships between classes. Along with previously reported wheat wax compound classes (fatty acids, 1-alkanols, 1-alkanol esters, aldehydes, alkanes, β-diketone, hydroxy-β-diketones, alkylresorcinols and methyl alkylresorcinols), esters of 2-alkanols and three types of aromatic esters (benzyl, phenethyl and p-hydroxyphenethyl) are also reported. In particular, 2-heptanol esters were identified. Detailed analyses of the isomer distributions within 1-alkanol and 2-alkanol ester homologs revealed distinct patterns of esterified acids and alcohols, suggesting several wax ester synthases with very different substrate preferences in both wheat organs. Terpenoids, including two terpenoid esters, were present only in peduncle wax. PMID:27264640

  15. Mutated clones of sweet orange cv. pera with late ripening obtained through mutation induction

    International Nuclear Information System (INIS)

    Sweet orange cv. Pera (Citrus sinensis L. Osbeck) harvested from July to November, is the most important cultivar in growing area and consumption in Brazil. To obtain mutants for many characteristics, one-year-old shoots were irradiated at 40 Gy of gamma-rays and the axillary buds were budded. After two cutting-backs, about 7,580 V3M1 plants were obtained. Total of 127 putative mutants were selected from these plants for further examination. The main purpose of this experiment was to obtain late ripening clones which have good fruit quality. These all plants were divided into 15 groups based on the characteristics and random blocks design with 5 replications, including one control plant in each block, was used. The plants were planted 4 X 7 meters and were grown in field condition without artificial irrigation. Ten fruits from each tree were used in evaluating the fruit characteristics such as contents of soluble solid (TSS), acidity, ratio of juice content and skin color, for four years. The data was analyzed by Dunnett test using SAS program. Six clones (9, 10, 16, 21, 58 and 84) were considered as late ripening mutants because they showed lower level of TSS or less ratio of juice content, comparing with the control, in more than one evaluation. The color of the skin of some mutants indicates that they have later ripening characteristics. Other agronomic characteristics are under evaluation in order to see if these mutants can be released as new cultivars

  16. Optimization of Freeze Drying Conditions for Purified Pectinase from Mango (Mangifera indica cv. Chokanan Peel

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2012-03-01

    Full Text Available Response surface methodology (RSM along with central composite design (CCD was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan peel. The effect of pectinase content (−2.66, 62.66 mg/mL, Arabic gum (−1.21, 10.21%, w/v, and maltodextrin (0.73, 7.26%, w/v as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05 effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v of Arabic gum, and 4 (%, w/v of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL, yield (86.4% and storage stability (84.2% of encapsulated pectinase were achieved.

  17. An Efficient Agrobacterium-Mediated Transformation of Strawberry cv. Camarosa by a Dual Plasmid System

    Directory of Open Access Journals (Sweden)

    Fatemeh Haddadi

    2015-02-01

    Full Text Available An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100% of shoot formation and the highest mean number of shoots (24 produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86% in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet.

  18. Nutritional disorder of lettuce cv. Veronica in nutrient solution with suppressed macronutrients

    Directory of Open Access Journals (Sweden)

    Thiago Batista Firmato de Almeida

    2011-06-01

    Full Text Available Lettuce is the most popular of the leafy vegetables. It is known worldwide and its consumption occurs mainly in the natural form. The objective was to evaluate the effect of macronutrient omission on the growth and nutritional status of the lettuce cv. Veronica, and to describe the visual symptoms of nutritional deficiency. The treatments complete consisted of the solution and the individual omission of N, P, K, Ca, Mg and S, under a completely randomized design with three replications. Plants were grown in Hoagland & Arnon, in pots (8L. At 56 days after transplant we evaluated plant height, leaf number, leaf area, SPAD index, dry matter of shoots, roots and whole plant, levels of macronutrients in shoots and roots, and the nature of nutritional disorders. The omission of nutrients affected the growth variables. The nutrients found in lettuce plants from nutrient solution and the complete omission in the shoot were, respectively, N= 23.2 to 9.5, P= 5.4 to 1.3, K= 58,9 to 3.2, Ca= 12.1 to 3.6, Mg= 5.5 to 0.7 and S= 3.2 to 1.5g.kg-1. The omission of macronutrients caused quality losses, since it affected the nutrition of the vegetables and this resulted in morphological changes, reflected as symptoms of deficiency for each nutrient.

  19. Responses of arching and traditional training systems to shading in cut rose (Rosa hybrida cv. Avalanche

    Directory of Open Access Journals (Sweden)

    A. Dolatkhahi

    2014-07-01

    Full Text Available   Nowadays, various training systems are utilized to grow greenhouse roses. Some of the training systems permit high canopy light penetration and hence increase canopy photosynthesis rate. The aim of this investigation was to evaluate the response of arching and traditional training systems to shading (0 (no shading/control, 25, 50 and 65% and their effects on marketing quality of cut roses (Rosa hybrid.cv. ‘Avalanche’, in the greenhouse condition. The experiment was carried out as split plots, based on completely randomized blocks design, with four replications and two plants assigned to each experimental unit. Results showed that training systems affected some qualitative traits. Plants trained with arching method produced longer flowering stems with higher fresh and dry weight and also higher leaf area over decapitated shoots. In both training systems, no significant difference was observed in time of sprouting, flower shoot diameter and specific leaf area. Increased shading significantly reduced shoot diameter and shoot fresh and dry weight; while it increased specific leaf area. However, shading had no significant effect on shoot length and leaf area. Based on the results of this experiment, application of arching training method could lead to improvement in qualitative properties of cut roses compared to traditional method under low light intensity and/or shade stress.

  20. Sward structure and livestock performance in guinea grass cv: Tanzania pastures managed by rotational stocking strategies

    Directory of Open Access Journals (Sweden)

    Valéria Pacheco Batista Euclides

    2014-12-01

    Full Text Available Grazing strategy is a key element in the determination of sward structure, herbage nutritive value and animal performance. We aimed to compare the herbage characteristics and performance of livestock in pastures of Panicum maximum cv. Tanzania managed, using two rotational stocking strategies, which provided either a fixed-length rest period (FRP of 35 days in the spring and fall and 30 days in the summer, or a variable-length rest period (VRP, determined by the time required for the canopy to achieve 70 cm in height. The pastures were evaluated in the pregrazing condition for forage mass (FM; leaf (LP, stem (SP and dead matter (DP percentages; and nutritive value (NV. The animals were weighed every 28 days. Pastures managed with the FRPs exhibited greater FMs, SPs and DPs and lower LPs and NVs than those managed with the VRPs. The average daily livestock weight gain was greater during the spring and summer for the VRP than for the FRP pastures, resulting in an average animal weight gain per area of 990 and 860 kg ha−¹ wet period−¹ for the pastures managed with the VRPs and FRPs, respectively. Thus, pasture rest periods that were maintained after the sward reached 70 cm in height reduced the animal performance on Tanzania guinea grass.

  1. Composition of pectic polysaccharides in a Portuguese apple (Malus domestica Borkh. cv Bravo de Esmolfe

    Directory of Open Access Journals (Sweden)

    Ana S. Silva

    2014-08-01

    Full Text Available Malus domestica Borkh. cv Bravo de Esmolfe is a typical Portuguese apple cultivar classified as Protected Designation and Origin (PDO. It is a traditional product produced under strict conditions and labelled with a specific law protected designation. This cultivar presents quite good sweetness and flavor. The monosaccharide composition of the pectic polysaccharides from this traditional apple is herein reported for the first time. Based on the molar ratios obtained from the sugar composition, the presumable pectin structure could be inferred. The cell-wall polysaccharides present in the alcohol-insoluble residue (AIR of unpeeled BE apple were sequentially fractionated. In addition, pectic material was also extracted by citric acid treatment prior to heat extraction at acidic pH. The water soluble pectin, imidazole soluble pectin and sodium carbonate soluble pectin account for 44, 16 and 40 % of the AIR, respectively. The pectic polysaccharides extracted in the presence of citric acid had lower galacturonic acid content and higher neutral sugars content. The homogalacturonan (HG and less-substituted rhamnogalacturonan (RG domains are extracted first. Pectin treated with citric acid has been shown to contain more substituted polymers, especially RG-I. In addition, the relatively higher Xylose/Galacturonic acid ratio found in the citric acid extract demonstrates that the xylogalacturonan (XG domain presumably is present in the pectic material of the unpeeled BE apple.

  2. Influence of vine vigor on grape (Vitis vinifera L. Cv. Pinot Noir) and wine proanthocyanidins.

    Science.gov (United States)

    Cortell, Jessica M; Halbleib, Michael; Gallagher, Andrew V; Righetti, Timothy L; Kennedy, James A

    2005-07-13

    The relationships between variations in grapevine (Vitis vinifera L. cv. Pinot noir) growth and resulting fruit and wine phenolic composition were investigated. The study was conducted in a commercial vineyard consisting of the same clone, rootstock, age, and vineyard management practices. The experimental design involved monitoring soil, vine growth, yield components, and fruit composition (soluble solids, flavan-3-ol monomers, proanthocyanidins, and pigmented polymers) on a georeferenced grid pattern to assess patterns in growth and development. Vine vigor parameters (trunk cross-sectional area, average shoot length, and leaf chlorophyll) were used to delineate zones within both blocks to produce research wines to investigate the vine-fruit-wine continuum. There was no significant influence of vine vigor on the amount of proanthocyanidin per seed and only minimal differences in seed proanthocyanidin composition. However, significant increases were found in skin proanthocyanidin (mg/berry), proportion of (-)-epigallocatechin, average molecular mass of proanthocyanidins, and pigmented polymer content in fruit from zones with a reduction in vine vigor. In the wines produced from low-vigor zones, there was a large increase in the proportion of skin tannin extracted into the wine, whereas little change occurred in seed proanthocyanidin extraction. The level of pigmented polymers and proanthocyanidin molecular mass were higher in wines made from low-vigor fruit compared to wines made from high-vigor fruit, whereas the flavan-3-ol monomer concentration was lower.

  3. Sequence analysis of ORF IV RTBV isolated from tungro infected Oryza sativa L. cv Ciherang

    Science.gov (United States)

    Hastilestari, Bernadetta Rina; Astuti, Dwi; Estiati, Amy; Nugroho, Satya

    2015-09-01

    The Effort to increase rice production is often constrained by pest and disease such as Tungro. The Tungro disease is caused by the joint infection with two dissimilar viruses; a bacil-form-DNA virus, the Rice tungro bacilliform virus(RTBV) and the spherical RNA virus, Rice tungro spherical virus (RTSV) and transmitted by Green leafhopper (Nephotettix virescens). The symptom of disease is caused by the presence of RTBV. The genome of RTBV consists of four Open reading frames (ORFs) which encode functional proteins. Of the four, ORF IV is unique because it exists only in RTBV. The most efficient method of generating disease resistance plants is to look for natural sources of resistance genes in wild or germplasm and then transfer the gene and the accompanying resistance in cultivated crop varieties. The aim of this study is, therefore, to isolate and analyze of 1170 bp gene of ORF 4 of Tungro virus isolated from an Indonesian rice cultivar, Ciherang (Oryza sativa L. cv Indica). DNA sequencing analysis using BLAST showed 94% similarity with the reference sequence gen bank Acc.M65026.1. The comparisons and mutation analysis of DNA sequences were discussed in this research.

  4. The BANANA project. V. Misaligned and precessing stellar rotation axes in CV Velorum

    Energy Technology Data Exchange (ETDEWEB)

    Albrecht, Simon; Winn, Joshua N.; Triaud, Amaury [Department of Physics, and Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Torres, Guillermo [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Fabrycky, Daniel C. [Department of Astronomy and Astrophysics, University of California Santa Cruz, Santa Cruz, CA 95064 (United States); Setiawan, Johny [Max-Planck-Institut für Astronomie, Königstuhl 17, D-69117 Heidelberg (Germany); Gillon, Michaël; Jehin, Emmanuel [Institut d' Astrophysique et de Géophysique, Université de Liège, Allée du 6 Août 17, Bat. B5C, Liège 1 (Belgium); Queloz, Didier [Observatoire Astronomique de lÚniversité de Genève, Chemin des Maillettes 51, Sauverny CH-1290 (Switzerland); Snellen, Ignas [Leiden Observatory, Leiden University, Niels Bohrweg 2, 2333 CA Leiden (Netherlands); Eggleton, Peter [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94551 (United States)

    2014-04-20

    As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of β{sub p} = –52° ± 6° and β{sub s} = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i {sub *}) over the last 30 yr, leading to a model in which the primary star's obliquity is ≈65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i {sub *}. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary.

  5. Structural Insight into Cell Wall Architecture of Micanthus sinensis cv. using Correlative Microscopy Approaches.

    Science.gov (United States)

    Ma, Jianfeng; Lv, Xunli; Yang, Shumin; Tian, Genlin; Liu, Xing'e

    2015-10-01

    Structural organization of the plant cell wall is a key parameter for understanding anisotropic plant growth and mechanical behavior. Four imaging platforms were used to investigate the cell wall architecture of Miscanthus sinensis cv. internode tissue. Using transmission electron microscopy with potassium permanganate, we found a great degree of inhomogeneity in the layering structure (4-9 layers) of the sclerenchymatic fiber (Sf). However, the xylem vessel showed a single layer. Atomic force microscopy images revealed that the cellulose microfibrils (Mfs) deposited in the primary wall of the protoxylem vessel (Pxv) were disordered, while the secondary wall was composed of Mfs oriented in parallel in the cross and longitudinal section. Furthermore, Raman spectroscopy images indicated no variation in the Mf orientation of Pxv and the Mfs in Pxv were oriented more perpendicular to the cell axis than that of Sfs. Based on the integrated results, we have proposed an architectural model of Pxv composed of two layers: an outermost primary wall composed of a meshwork of Mfs and inner secondary wall containing parallel Mfs. This proposed model will support future ultrastructural analysis of plant cell walls in heterogeneous tissues, an area of increasing scientific interest particularly for liquid biofuel processing. PMID:26358178

  6. Folate levels and polyglutamylation profiles of papaya (Carica papaya cv. Maradol) during fruit development and ripening.

    Science.gov (United States)

    Ramos-Parra, Perla A; García-Salinas, Carolina; Hernández-Brenes, Carmen; de la Garza, Rocío I Díaz

    2013-04-24

    Folates are essential micronutrients for humans, and their deficiency causes several detrimental effects on human health. Papaya fruit is an important natural source of some micronutrients. This paper presents a first complete characterization of folate derivatives accumulated in cv. Maradol papaya during fruit development and ripening processes. During postharvest ripening, the fruit accumulated up to 24.5% of the daily folate recommended dietary allowance (RDA) for an adult in a 1 cup (145 g) portion. Tetrahydrofolate (THF) and 5-methyl-THF were the predominant folate classes observed. Surprisingly, an unusually long polyglutamylation profile of tentatively up to 17 glutamates linked to 5-methyl-THF was detected; to the authors' knowledge, this very long polyglutamyl tail has not been reported for any organism, and it is probably characteristic of this plant species. This polyglutamylation degree changed throughout fruit development and ripening, showing the largest differences at the onset of ripening. This work raises questions about the functional role of folate derivatives in fruit development.

  7. Nitrogen and potassium fertilization in yield and macronutrients contents of heliconia cv. Golden Torch

    Directory of Open Access Journals (Sweden)

    Márkilla Z. Beckmann-Cavalcante

    2016-04-01

    Full Text Available ABSTRACT This study aimed to evaluate the effect of nitrogen (N and potassium (K fertilization in yield and contents of macronutrients in heliconia plants (Heliconia psittacorum x Heliconia spathocircinata Aristeguieta cv. Golden Torch. The experiment was set in a randomized block design, in a 4 x 4 factorial, corresponding to N doses (0, 120, 180 and 240 g of N hole-1 and K doses (0, 120, 180 and 240 g of K2O hole-1 with four replicates and five rhizomes per plot, under field conditions. After one year of cultivation, yield (number of flower stems per square meter and the leaf contents of N, P, K, Ca, Mg and S were determined. The applied N doses did not influence either yield or the leaf contents of the evaluated macronutrients. K fertilization favored the increase in leaf K content and the decrease in the contents of P, Ca and Mg in leaves of heliconias. Under the conditions of this experiment, it is recommended to apply 161.46 g of K2O hole-1 for a maximum yield of 9.89 stems m-2 per month.

  8. One central heating boiler for all combustible gases; Een CV-ketel voor alle brandbare gassen

    Energy Technology Data Exchange (ETDEWEB)

    Gersen, S.; Darmeveil, H.; Hegge, R. [DNV KEMA Energy and Sustainability, Arnhem (Netherlands)

    2012-06-07

    There is increasing interest in the distribution of sustainable gases (H2, H2/CO, CH4/CO2) and imported gases, such as LNG. The composition of these 'new' gases can differ greatly from the traditional distributed gases. The combustion characteristics may cause undesired effects in household appliances. One of the solutions is to develop equipment that can accept a wide range of gases and mixtures thereof. To this end, within the EDGaR-program (Energy Delta Gas Research) the project 'new gas sensors' is started by the Energy Research Centre of the Netherlands (ECN), Delft University of Technology (TUD) and DNV-KEMA/Gasunie to develop a boiler in which the new gases can be used [Dutch] Er is toenemende interesse in de distributie van duurzame gassen (H2 , H2/CO, CH4/CO2 ) en geimporteerde gassen, zoals LNG. De samenstelling van deze 'nieuwe' gassen kan sterk verschillen van de traditioneel gedistribueerde gassen. De verbrandingseigenschappen kunnen ongewenste effecten veroorzaken in huishoudelijke apparatuur. Een van de oplossingen is het ontwikkelen van apparatuur die een breed scala aan gassamenstellingen kan accepteren. Hiertoe is binnen het EDGaR-programma (Energy Delta Gas Research) een project 'new gas sensors' gestart met ECN, TU Delft en DNV-KEMA/Gasunie voor het ontwikkelen van een CV-ketel die geschikt is voor de nieuwe gassen.

  9. Transpiration, crop coefficient and water use of Olive tree (cv. Cordovil) in Southern Portugal

    Science.gov (United States)

    Santos, F. L.

    2008-12-01

    Orchard olive transpiration, soil water status and stomatal response in relation to water deficit were investigated to clarify mechanisms of tree water uptake and stomatal control to improve the irrigation scheduling of low-density olive trees of cv. Cordovil grown in typical Mediterranean environment of Southern Portugal. Trees were subject to three irrigation treatments. Treatment A received 100% of crop evapotranspiration by a drip irrigation system, a sustained deficit (SDI)treatment B received 60% of crop evapotranspiration, a regulated deficit(RDI) irrigation treatment C received irrigation water before-flowering and just before pit-hardening, and a Dry-farming treatment. Tree and orchard transpiration and the dynamics of water uptake by roots were estimated from sap flow measurements and water balance technique. Stomatal conductance was modeled from local meteorological variables, measured sap flow and tree canopy variables. Higher than treatment A and B stomatal conductance and the high tree fruit production recommend treatment C as most suitable for scheduling irrigation of olive orchards in wet years of well distributed late summer rainfall. For drier years of no summer and early autumn rains that minimizes available water to extract by roots outside the wet bulb of drip irrigation and for the scarce readily available irrigation water years, as so often occurs in the region, the sustained deficit irrigation (SDI) regime seems a better option. Nonetheless, for years of limited available water resources that preclude sustained deficit irrigation, careful management of the proposed RDI could also allow for efficient use of irrigation water.

  10. Rhizospheric Microflora Escalating Aroma Constituents and Yield Attributes in Ocimum tenuiflorum (L. cv. CIM-Ayu

    Directory of Open Access Journals (Sweden)

    Shilpi Khare Saikia

    2014-01-01

    Full Text Available The exploration of rhizospheric microbial flora for crop yield enhancement is well established. Rhizospheric microbes influence the plant physiology by imparting several beneficial effects, namely, Nitrogen fixation, increased nutrient uptake, and secondary metabolites production on their host plants. The present study investigates the response of Bacillus megaterium ATCC No. 13525, Pseudomonas fluorescens ATCC No. 14581, and Trichoderma viride MTCC No. 167 in alone and combined treatments for their effect on growth and yield parameters in a commercially important Ocimum tenuiflorum L. cv. CIM-Ayu. The plant is therapeutically important for its essential oil constituents, namely, eugenol, β-caryophyllene, and various monoterpenes. The combination treatments, T7 (B. megaterium + P. fluorescens and T8 (B. megaterium + P. fluorescens + T. viride, showed maximum enhancement (27.27% of percentage essential oil as compared to untreated control. Nutrient uptake especially N2 content was significantly increased (43% with the treatment T8 (B. megaterium + P. fluorescens + T. viride. Amongst major essential oil constituents, eugenol content was maximally increased by 58.5% as compared to 42.9% (control indicating a cumulative role of microbial inoculants for crop yield boost-up.

  11. Application of microbiological fertilizers in viticulture: Grape yield and quality of wine cv. Riesling

    Directory of Open Access Journals (Sweden)

    Sivčev Branislava

    2005-01-01

    Full Text Available The recommended cultivars for top quality wines Riesling in the vineyards of Grocka is in full crop. It was grafted on Kober 5 BB stock and planted on the soil type cambysoil. The content of total nitrogen is 0.1-0.15%. Supply of easily available potassium varies between 12.3-15 mg/100g a.d.s.2, i.e. phosphorus 0.4-3.6 mg/100g a.d.s. in layer up to 40 cm. Microbiological fertilizer was used in the study - biological preparation prepared with mixed natural populations Azotobacter chroococcum, Bacillus megaterium and Bacillus circulons. The space in row is idle land and the space between rows was sown each year (March-April with a mixture of field pea and barley and ploughed in the inflorescence phase of legumes. Grape yield varied between 8772-6804 kg/ha. Microbiological fertilizer with Azotobacter had the highest yield and the control treatment had the lowest yield, where only grass mixture was sown. Extremely dry climatic conditions in the trial period caused the grape yield in cv. Riesling to be extremely low. In combination of fertilizers Bacillus megaterium + Bacillus circulons wine with the most ethanol, extracts and polyphenols was obtained. The wine obtained from the control treatment had a typical taste.

  12. Growth and Respiratory Response of Fig (Ficus carica L. cv. Mission) Fruits to Ethylene.

    Science.gov (United States)

    Marei, N; Crane, J C

    1971-09-01

    Growth in diameter of the fig (Ficus carica L. cv. Mission) fruit takes place in three distinct periods; two periods (I and III) of rapid growth are separated by a period (II) of slow growth. With respect to exposure to ethylene, the fruit exhibits a two phase response. Ethylene inhibits fruit growth in phase A (period I), the period of cell division, stimulates growth in early phase B (early period II), and stimulates both growth and ripening during the remainder of phase B (late period II and period III). The adverse effect of exogenous ethylene on fruits during phase A is thought to be due to inhibition of cell division. The gradual transition occurring in the response of fruits during phase B was interpreted in terms of carbohydrate level in the fruits.The onset of period III and a respiratory climacteric rise was preceded by or concomitant with a sudden burst of endogenous ethylene synthesis. This, together with the fact that exogenous ethylene applied at the proper stage of fruit growth triggers both ripening and the climacteric rise, leads to the conclusion that ethylene is the causal agent. In other words, the data support the concept that ethylene is a growth hormone that initiates a chain of metabolic and physiological events leading to fig fruit ripening.

  13. Purification and modes of antifungal action by Vicia faba cv. Egypt trypsin inhibitor.

    Science.gov (United States)

    Fang, Evandro Fei; Hassanien, Abdallah Abd Elazeem; Wong, Jack Ho; Bah, Clara Shui Fern; Soliman, Saeed Saad; Ng, Tzi Bun

    2010-10-13

    A new 15 kDa Bowman-Birk type trypsin inhibitor (termed VFTI-E1) from fava beans (Vicia faba cv. Egypt 1) was isolated using liquid chromatography. Though it exhibited substantial homology in N-terminal amino acid sequence to other protease inhibitors, VFTI-E1 showed antiproteolytic activity against trypsin (K(i) 11.9 × 10(-9) M) but hardly any activity against chymotrypsin. It demonstrated antifungal activity toward the filamentous fungus Valsa mali with an IC(50) of 20 μM. The mechanism of its antifungal action toward V. mali included (1) induction of alteration of hyphal morphology, (2) growth inhibition by chitin deposition at hyphal tips, and (3) permeabilization of fungal membrane. The antifungal activity of VFTI-E1 was dependent on the ambient ionic strength as increasing concentrations of NaCl, CaCl(2), and MgCl(2) diminished the activity. The membranolytic action of VFTI-E1 was confined to fungus, but not exerted on human and rabbit erythrocytes. This study sheds light on the mode of hyphal growth inhibitory activity of protease inhibitors with antifungal activity. The antifungal activity of VFTI-E1 amplifies the scope of its potential applications. PMID:20836498

  14. Agrobacterium-mediated transformation of Vitis Cv. Monastrell suspension-cultured cells: Determination of critical parameters.

    Science.gov (United States)

    Chu, Mingyu; Quiñonero, Carmen; Akdemir, Hülya; Alburquerque, Nuria; Pedreño, María Ángeles; Burgos, Lorenzo

    2016-05-01

    Although some works have explored the transformation of differentiated, embryogenic suspension-cultured cells (SCC) to produce transgenic grapevine plants, to our knowledge this is one of the first reports on the efficient transformation of dedifferentiated Vitis vinifera cv Monastrell SCC. This protocol has been developed using the sonication-assisted Agrobacterium-mediated transformation (SAAT) method. A construct harboring the selectable nptII and the eyfp/IV2 marker genes was used in the study and transformation efficiencies reached over 50 independent transformed SCC per gram of infected cells. Best results were obtained when cells were infected at the exponential phase. A high density plating (500 mg/dish) gave significantly better results. As selective agent, kanamycin was inefficient for the selection of Monastrell transformed SCC since wild type cells were almost insensitive to this antibiotic whereas application of paromomycin resulted in very effective selection. Selected eyfp-expressing microcalli were grown until enough tissue was available to scale up a new transgenic SCC. These transgenic SCC lines were evaluated molecularly and phenotypically demonstrating the presence and integration of both transgenes, the absence of Agrobacterium contamination and the ability of the transformed SCC to grow in highly selective liquid medium. The methodology described here opens the possibility of improving the production of valuable metabolites. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:725-734, 2016.

  15. An efficient Agrobacterium-mediated transformation of strawberry cv. Camarosa by a dual plasmid system.

    Science.gov (United States)

    Haddadi, Fatemeh; Aziz, Maheran Abd; Abdullah, Siti Nor Akmar; Tan, Soon Guan; Kamaladini, Hossein

    2015-02-23

    An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100%) of shoot formation and the highest mean number of shoots (24) produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86%) in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet.

  16. Highly efficient Agrobacterium-mediated transformation of banana cv. Rasthali (AAB) via sonication and vacuum infiltration.

    Science.gov (United States)

    Subramanyam, Kondeti; Subramanyam, Koona; Sailaja, K V; Srinivasulu, M; Lakshmidevi, K

    2011-03-01

    A reproducible and efficient transformation method was developed for the banana cv. Rasthali (AAB) via Agrobacterium-mediated genetic transformation of suckers. Three-month-old banana suckers were used as explant and three Agrobacterium tumefaciens strains (EHA105, EHA101, and LBA4404) harboring the binary vector pCAMBIA1301 were used in the co-cultivation. The banana suckers were sonicated and vacuum infiltered with each of the three A. tumefaciens strains and co-cultivated in the medium containing different concentrations of acetosyringone for 3 days. The transformed shoots were selected in 30 mg/l hygromycin-containing selection medium and rooted in rooting medium containing 1 mg/l IBA and 30 mg/l hygromycin. The presence and integration of the hpt II and gus genes into the banana genome were confirmed by GUS histochemical assay, polymerase chain reaction, and southern hybridization. Among the different combinations tested, high transformation efficiency (39.4 ± 0.5% GUS positive shoots) was obtained when suckers were sonicated and vacuum infiltered for 6 min with A. tumefaciens EHA105 in presence of 50 μM acetosyringone followed by co-cultivation in 50 μM acetosyringone-containing medium for 3 days. These results suggest that an efficient Agrobacterium-mediated transformation protocol for stable integration of foreign genes into banana has been developed and that this transformation system could be useful for future studies on transferring economically important genes into banana.

  17. A comparative analysis of a fuzzless-lintless mutant of Gossypium hirsutum L. cv. Xu-142

    Institute of Scientific and Technical Information of China (English)

    于晓红; 朱勇清; 卢山; 张天真; 陈晓亚; 许智宏

    2000-01-01

    A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovules. RT-PCR analysis showed that the steady-state levels of transcripts of the fiber-specific E6 and Expansin genes were high in wild type (WT) ovules during the fiber initiation and elongation stages, and peaked around 15 days post anthesis (DPA), but only a trace amount of these transcripts was detectable in fl ovules of alt developmental stages investigated. CotmybA, a member of the Myb family, exhibited a clear expression in developing WT ovules, but the expression was abnormal in fl ovules. Application of GA3, or GA3 plus IAA, to the culture medium rescued in vitro fiber initiation and growth of fl ovules only partially. In addition, transcription of E6 and Expansin genes of in vitro cultured WT and fl ovules responded similarly to exogenous hormones. The hormones had less effect on Cot

  18. A comparative analysis of a fuzzless-lintless mutant of Gossypium hirsutum L. cv. Xu-142

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovules. RT-PCR analysis showed that the steady-state levels of transcripts of the fiber-specific E6 and Expansin genes were high in wild type (WT) ovules during the fiber initiation and elongation stages, and peaked around 15 days post anthesis (DPA), but only a trace amount of these transcripts was detectable in fl ovules of all developmental stages investigated. CotmybA, a member of the Myb family, exhibited a clear expression in developing WT ovules, but the expression was abnormal in fl ovules. Application of GA3, or GA3 plus IAA, to the culture medium rescued in vitro fiber initiation and growth of fl ovules only partially. In addition, transcription of E6 and Expansin genes of in vitro cultured WT and fl ovules responded similarly to exogenous hormones. The hormones had less effect on CotmybA transcription in ovules cultured in vitro, and again the WT and fl ovules showed a similar expression. These results suggest that the abnormal or extremely low level of expression of E6, Expansin and CotmybA genes in developing fl seeds is related to the absence of seed-hairs, and the mechanism underlying needs further investigation.

  19. Entropy and coefficient of variation (CV as tools for assessing power quality

    Directory of Open Access Journals (Sweden)

    J. H. Estrada

    2011-10-01

    Full Text Available This paper presents several concepts and techniques for quantifying electric signal entropy and variability for assessing power quality, showing their irregularities, such as the electrical sine wave which delivers power to industry and homes. The paper will review these concepts and show some magnetic radiated waves measurements taken below 115 KV electricity line circuits in the city of Manizales (Colombia through non-invasive and indirect measurement of the complexity of the current transported by the supply. Entropy, as an accepted indicator of physical complexity, is proportional to the logarithm of the number of states in a thermodynamic system. Some writers have identified entropy as being system disorder, but this is not completely true. Entropy of information was first defined by Shannon in 1949 and it is applied to many physical events. Entropy has been used for describing the complexity of the magnetic wave radiated by the line in this paper. Furthermore, the magnetic waves’ spectral and amplitude coefficient of variation (CV were obtained using a multiresolution wavelet algorithm which measures data distribution dispersion. It is a dimensionless number allowing the variation of magnetic radiated data having significantly different mean values to be compared to a 60 Hz pure magnetic wave as reference.

  20. Characterisation of chlorophyll oxidation mediated by peroxidative activity in olives (Olea europaea L.) cv. Hojiblanca.

    Science.gov (United States)

    Vergara-Domínguez, Honorio; Roca, María; Gandul-Rojas, Beatriz

    2013-08-15

    The oxidation of chlorophyll a (chl a) catalysed by peroxidase (POD) from mesocarp of the olive fruit (Olea europaea L., cv Hojiblanca) in the presence of H2O2 and 2,4-dichlorophenol (2,4-DCP), is characterised via the individualised quantification of the products of the enzymatic reaction using a new methodology of HPLC-UV spectrometry. This innovation has allowed the discovery that, in addition to 13(2) OH chl a and 15(1) OH lactone chl a, which are the first products of POD on chl a, the reaction process sequentially creates another series of oxidised chlorophyll derivatives which have not been previously described. Their origins have been linked to POD activity in the presence of 2,4-DCP. Likewise, a study of the effect of the concentration of the various cosubstrates on the POD reaction rate demonstrated that the correct establishment of the relative concentrations of the same ([H2O2]/[2,4-DCP]/[Chl]=1:3:0.02) is crucial to explaining inhibition effects by substrates and carrying out optimum measurements. Therefore, new essential parameters for the determination of POD activity on a chlorophyll substrate are established. PMID:23561174

  1. 利用 OpenCV 实现在 Android系统上的图像边缘检测%Implementation of Image Edge Detection on Android Based on OpenCV

    Institute of Scientific and Technical Information of China (English)

    杨平

    2014-01-01

    A method of image edge detection based on OpenCV on Android system is presented .How to set up Android NDK environment is imtroduced .JNI is used to call the Canny function in OpenCV and generate the dynamic link library . The result of experiment shows a good performance of the image edge detection program on Android system .%论文提出了利用OpenCV实现在Android系统上图像边缘检测的方法。介绍了Android NDK开发环境的搭建,利用JNI调用OpenCV中的Canny算子,采用Android NDK生成动态链接库。实验结果表明在Android系统上图像边缘检测功能和性能良好。

  2. Pathogenicity of seed-borne and seedling fungi of groundnut cv. Tatu/
    Patogenicidade de fungos associados às sementes e plântulas de amendoim cv. Tatu

    OpenAIRE

    Débora Cristina Santiago; Édison Miglioranza; Romeu Munashi Endo; Nair Mieko Takaki Bellettini

    2005-01-01

    Seeds and seedlings of groundnut (Arachis hypogaea) cv. Tatu were investigated under laboratory and green house conditions to detect and identify any seed-borne pathogenic fungi present, besides determining the pathogenicity and the seed damages. The fungi detected were Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. and Phoma sp.. The most frequently isolated fungi were Aspergillus niger and Penicillium sp.. These fungi were tra...

  3. CvADH1, a member of short-chain alcohol dehydrogenase family, is inducible by gibberellin and sucrose in developing watermelon seeds.

    Science.gov (United States)

    Kim, Joonyul; Kang, Hong-Gyu; Jun, Sung-Hoon; Lee, Jinwon; Yim, Jieun; An, Gynheung

    2003-01-01

    To understand the molecular mechanisms that control seed formation, we selected a seed-preferential gene (CvADH1) from the ESTs of developing watermelon seeds. RNA blot analysis and in situ localization showed that CvADH1 was preferentially expressed in the nucellar tissue. The CvADH1 protein shared about 50% homology with short-chain alcohol dehydrogenase including ABA2 in Arabidopsis thaliana, stem secoisolariciresinol dehydrogenase in Forsythia intermedia, and 3beta-hydroxysterol dehydrogenase in Digitalis lanata. We investigated gene-expression levels in seeds from both normally pollinated fruits and those made parthenocarpic via N-(2-chloro-4-pyridyl)-N'-phenylurea treatment, the latter of which lack zygotic tissues. Whereas the transcripts of CvADH1 rapidly started to accumulate from about the pre-heart stage in normal seeds, they were not detectable in the parthenocarpic seeds. Treating the parthenogenic fruit with GA(3) strongly induced gene expression, up to the level accumulated in pollinated seeds. These results suggest that the CvADH1 gene is induced in maternal tissues by signals made in the zygotic tissues, and that gibberellin might be one of those signals. We also observed that CvADH1 expression was induced by sucrose in the parthenocarpic seeds. Therefore, we propose that the CvADH1 gene is inducible by gibberellin, and that sucrose plays an important role in the maternal tissues of watermelon during early seed development. PMID:12552151

  4. Canny edge detection based on OpenCV in Ubuntu%Ubuntu下基于OpenCV的Canny边缘检测

    Institute of Scientific and Technical Information of China (English)

    许才敬

    2011-01-01

    详细地介绍了用于数字图像处理的开放源代码的计算机视觉类岸-OpenCV.利用OpenCV中的数字图像处理函数使复杂的问题变得简单化.介绍了OpenCV的特点和功能,讨论了在Ubuntu环境下如何配置OpenCV,最后在此基础上给出了一个CAnny边缘检测应用实例,其对数字图像处理的研究具有一定的实用价值.

  5. Internal flow Patterns of the Horizontal Heat Mode Closed-Loop Oscillating Heat Pipe with Check Valves (HHMCLOHP/CV

    Directory of Open Access Journals (Sweden)

    S. Sangiamsuk

    2013-01-01

    Full Text Available This research was to study the internal flow patterns on heat transfer rates of the Horizontal Heat Mode Closed Loop Oscillating Heat Pipe with Check Valves (HHMCLOHP/CV. The HHMCLOHP/CV was made from a Pyrex glass capillary tube with a 2.4 mm inside diameter. There were 10 meandering turns with 2 check valves. Ethanol and a silver nano-ethanol mixture were used as working fluid. Experimental results found that if working fluid varies from ethanol to a silver nano-ethanol mixture and the evaporator temperature increases the main flow patterns were Slug flow + Annular flow. The main regime of each flow pattern can be determined from the flow pattern map.

  6. Molecular mapping of powdery mildew resistance gene Eg-3 in cultivated oat (Avena sativa L. cv. Rollo).

    Science.gov (United States)

    Mohler, Volker; Zeller, Friedrich J; Hsam, Sai L K

    2012-05-01

    Powdery mildew is a prevalent fungal disease affecting oat (Avena sativa L.) production in Europe. Common oat cultivar Rollo was previously shown to carry the powdery mildew resistance gene Eg-3 in common with cultivar Mostyn. The resistance gene was mapped with restriction fragment length polymorphism (RFLP) markers from Triticeae group-1 chromosomes using a population of F(3) lines from a cross between A. byzantina cv. Kanota and A. sativa cv. Rollo. This comparative mapping approach positioned Eg-3 between cDNA-RFLP marker loci cmwg706 and cmwg733. Since both marker loci were derived from the long arm of barley chromosome 1H, the subchromosomal location of Eg-3 was assumed to be on the long arm of oat chromosome 17. Amplified fragment length polymorphism (AFLP) marker technology featured as an efficient means for obtaining markers closely linked to Eg-3.

  7. Normal photoresponses and altered b-wave responses to APB in the mdxCv3 mouse isolated retina ERG supports role for dystrophin in synaptic transmission

    OpenAIRE

    GREEN, DANIEL G.; Guo, Hao; PILLERS, DE-ANN M.

    2004-01-01

    The mdxCv3 mouse is a model for Duchenne muscular dystrophy (DMD). DMD is an X-linked disorder with defective expression of the protein dystrophin, and which is associated with a reduced b-wave and has other electroretinogram (ERG) abnormalities. To assess potential causes for the abnormalities, we recorded ERGs from pieces of isolated C57BL/6J and mdxCv3 mouse retinas, including measurements of transretinal and intraretinal potentials. The ERGs from the isolated mdxCv3 retina differ from tho...

  8. Elimination of browning exudate and in vitro development of shoots in Pistacia vera L. cv. mateur and Pistacia atlantica Desf. Culture

    OpenAIRE

    M. I. Trujillo; S. Mederos-Molina

    2014-01-01

    We report diminution and/or elimination of browning exudate followed by in vitro establishment of in Pistacia vera cv. mateur and Pistacia atlantica explants. Soaking P. vera cv. mateur explants prior to culture in L-cysteine HCl for 15 min (100 µM) inhibits blackening of the modified Murashige and Skoog medium - MS + 400 mg/l NH4NO3 - and of the explants; while shoot formation was increased. The browning in P. vera cv. mateur and P. atlantica explants dissolved when modified MS and Quoirin a...

  9. Effects of gamma irradiation and hot-water treatment on the shelf life and quality of Thai mango cv. Rad

    International Nuclear Information System (INIS)

    Preclimacteric mature-green mangoes cv. Rad were irradiated at doses up to 1 kGy after hot water treatment at 55 deg C for 5 min. Irradiation significantly reduced rotting, delayed colour development, preserved quality and extended shelf life. Irradiation injury increased with increasing dose and storage period. In terms of shelf life and quality, a hot-water treatment followed by irradiation at 0.3 kGy was found to be the best combination treatment

  10. [Leaf anatomy of the mosaic ficus benjamina cv. Starlight and interaction of source and sink chimera components].

    Science.gov (United States)

    Labunskaia, E A; Zhigalova, T V; Chub, V V

    2007-01-01

    Leaf anatomy was studied in the mosaic Ficus benjamina cv. Starlight and non-chimeric Ficus benjamina cv. Daniel. The number of chloroplasts in a white, chlorophyll-deficient tissue declines as compared to the green tissue. However, their functional activity is retained. The leaf of the mosaic F. benjamina contains two or, sometimes, three subepidermal layers. Mesophyll forms one layer in the green and white parts of leaf palisade and one white and one green layer in the transitional zone (edge). In the transitional zone, green spongy mesophyll is located between two white spongy layers and the proportion of photosynthesizing cells varies. In cv. Daniel, there are two subepidermal layers and one layer of columnar mesophyll cells. According to the morphometry data, the proportion of white zone in the leaf correlates with the leaf position in the whole shoot: the higher the branch order, the larger the proportion of white zone. The total leaf area depends also on its position in the shoot. No such correlation was found in non-chimeric F. benjamina cv. Daniel. In the mosaic chimera, the source-sink status appears to depend on the leaf position in the shoot. Experiments with individual shoots of the same order and elimination of all lateral shoots have shown that the proportion of white zone in new leaves on the shoot increases with the total area of green zone. Thus, the area of assimilating shoot surface affects the formation of leaves in the meristem. A hypothesis was put forward that the source-sink state affects the ratio of green and white parts in the leaf primordium. Products of photosynthesis (carbohydrates) are a possible metabolic signal affecting the meristem. It cannot be excluded as well that the hormonal state undergoes changes in the chimeric plant.

  11. Response of cotyledon explants of Capsicum annuum L. cv. kujawianka to chosen plant growth regulators in in vitro culture

    Directory of Open Access Journals (Sweden)

    Alicja Fraś

    2014-02-01

    Full Text Available Shoot buds originated directly on cotyledon explants of Capsicum annuum L. cv. Kujawianka, when Linsmaier and Skoog medium was enriched with BAP (2 mg/l. Kinetin (2 mg/l or kinetin with IAA (1 mg/l + 1 mg/l induced indirect shoot buds regeneration from callus. Rooting was obtained with explants cultivated on a medium containing NAA (0,5 mg/l. Occurrence of the early stages of differentiation was proved at the histological level.

  12. Karakterisasi Simplisia Dan Skrining Fitokimia Serta Uji Aktivitas Antioksidan Ekstrak Etanol Buah Paprika (Capsicum annum L. cv.group grossum)

    OpenAIRE

    Salviani, Helen

    2014-01-01

    Bell peppers (Capcisum annuum L. cv.group grossum) are one of the essential developing commodities today. Besides being used as a food purposes because it contains a fairly complete nutrients, such as proteins, fats, carbohydrates, minerals and vitamins, bell peppers also contain medicinal compounds to relieve pain and improving blood flow. The objective of the research was to determine the characteristics, phytochemical screening, percolation extraction and antioxidant activity of ethanol ex...

  13. Monitorando a Comunicação na CV-Muzar com o Uso de Agentes Inteligentes

    Directory of Open Access Journals (Sweden)

    Ana Carolina Bertoletti De Marchi

    2010-03-01

    Full Text Available This article describes a Multiagent System (MAS proposed to monitor conversations in the chat tool of the Muzar Virtual Community (Comunidade Virtual do Muzar, CV-Muzar. Thus, conversational markers and their use in synchronous chat environment are being analyzed to propose a compensation mechanism for the identified communication deficits. The proposed MAS has five cooperative agents that monitor conversations in real time and interfere with the chat interface to advice and, if necessary, warn the participant.

  14. Usulan Perancangan Fasilitas Kerja Untuk Mengurangi Musculoskeletal Disorder (MSDs) Pada Stasiun Pendempulan di CV.Super Plates

    OpenAIRE

    Silitonga, Winda Wahyuni

    2015-01-01

    CV. Super Plates adalah sebuah perusahaan yang memproduksi baterai/aki mobil. Proses pendempulan plat dalam produksi baterai mobil dilakukan dengan postur kerja berdiri. Penelitian ini bertujuan untuk mengkaji resiko pekerja dalam proses pendempulan dan mengusulkan perancangan fasilitas kerja yang ergonomis berdasarkan antropometri tubuh. Metode yang digunakan dalam penelitian ini adalah NBM, beban kerja berdasarkana denyut nadi pekerja, REBA (Rapid Entire Body Assessment), dan antropometri. ...

  15. Determination of an efficient irrigation schedule for the cultivation of rose cv. freedom under greenhouse conditions in colombia

    OpenAIRE

    Arévalo, Jhon Jairo; Vélez S., Javier Enrique; Intrigliolo, Diego Sebastiano

    2014-01-01

    An experiment on rose (Rosa sp.) cv. Freedom was performed in a greenhouse on the Bogota Plateau, Colombia, to identify an efficient irrigation regime for this crop. The tested treatments were based on three irrigation doses, applying different fractions of the estimated crop evapotranspiration (ETc), calculated using a class A evaporation tank: i) 100% ETc (ETc100), ii) 80% ETc (ETc80) and iii) 70% ETc (ETc70). During the entire experimental period, from mid-May to early September, the crop ...

  16. Soil respiration and carbon balance in a Moso bamboo (Phyllostachys heterocycla (Carr.) Mitford cv. Pubescens) forest in subtropical China

    OpenAIRE

    Tang X.; Fan S; Qi L; Guan F; Cai C; Du M

    2015-01-01

    Understanding spatial and temporal variation in soil respiration (RS) in different forest ecosystems is crucial to estimate the global carbon balance. Bamboo forest is a special forest type in southern China covering an area of 5.38 million ha, 70% of which are Moso bamboo (Phyllostachys heterocycla (Carr.) Mitford cv. Pubescens) forests. Bamboo forests contribute more than 10% to the whole carbon stock of forest ecosystems in China, and therefore play a critical role in the regional and nati...

  17. Analisis Pengaruh Gaya Kepemimpinan, Budaya Organisasi Dan Suasana Kerja Terhadap Kepuasan Kerja Karyawan Pada Percetakan CV. Wati Grafika Medan

    OpenAIRE

    Putra, M. Afianda

    2014-01-01

    Nowadays, business development is so competing that a company should be able to properly utilize its human resources in managing its business. In improving its employees’ work satifiscation, CV. Wati Grafika Medan, a printing company, should be managed by a dynamic, creative, and transparent leader, but he should still be critical and responsive to new ideas and changes so that the employees’optimal work satisfaction can be achieved. The purpose of this descriptive quantitative explanatory st...

  18. Psychometric Properties of the Obsessive-Compulsive Inventory-Child Version (OCI-CV in Chilean Children and Adolescents.

    Directory of Open Access Journals (Sweden)

    Agustín E Martínez-González

    Full Text Available In recent years, there has been a considerable increase in the development of assessment tools for obsessive-compulsive symptomatology in children and adolescents. The Obsessive Compulsive Inventory-Child Version (OCI-CV is a well-established assessment self-report, with special interest for the assessment of dimensions of Obsessive Compulsive Disorder (OCD. This instrument has shown to be useful for clinical and non-clinical populations in two languages (English and European Spanish. Thus, the aim of this study was to analyze the psychometric properties of the OCI-CV in a Chilean community sample. The sample consisted of 816 children and adolescents with a mean age of 14.54 years (SD = 2.21; range = 10-18 years. Factor structure, internal consistency, test-retest reliability, convergent/divergent validity, and gender/age differences were examined. Confirmatory factor analysis showed a 6-factor structure (Doubting/Checking, Obsessing, Hoarding, Washing, Ordering, and Neutralizing with one second-order factor. Good estimates of reliability (including internal consistency and test-retest, evidence supporting the validity, and small age and gender differences (higher levels of OCD symptomatology among older participants and women, respectively are found. The OCI-CV is also an adequate scale for the assessment of obsessions and compulsions in a general population of Chilean children and adolescents.

  19. Improved antioxidant and anti-inflammatory potential in mice consuming sour cherry juice (Prunus Cerasus cv. Maraska).

    Science.gov (United States)

    Sarić, Ana; Sobocanec, Sandra; Balog, Tihomir; Kusić, Borka; Sverko, Visnja; Dragović-Uzelac, Verica; Levaj, Branka; Cosić, Zrinka; Macak Safranko, Zeljka; Marotti, Tatjana

    2009-12-01

    The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties. PMID:19763832

  20. OpenCV在车牌识别中的应用%Application of OpenCV in License Plate Recognition

    Institute of Scientific and Technical Information of China (English)

    姜莉莉; 蒋潇; 陈昕怡; 殷旭东

    2014-01-01

    For license plate recognition on smart phone, application of OpenCV is studied. First, OpenCV and work flow of license plate recognition are introduced. Then detailed application of OpenCV is discussed, which is in license plate location, character segmentation and character recognition. Experimental results show that the recognition is good. The foundation is laid for further developing a vehicle information management system on smart phones such as Android.%为利用智能手机进行车牌识别,研究了OpenCV在车牌识别中的应用。首先介绍了OpenCV及车牌识别的工作流程,然后论述了OpenCV在车牌定位、字符分割和字符识别中的具体应用。实验结果表明识别效果良好,为在Android等智能手机上进一步开发车辆信息管理系统奠定了基础。

  1. Analisa dan Perancangan Sistem Informasi Penjualan Online (E-Commerce pada CV Selaras Batik Menggunakan Analisis Deskriptif

    Directory of Open Access Journals (Sweden)

    Himawan -

    2015-05-01

    Full Text Available Batik merupakan salah satu pakaian tradisional yang menjadi ciri khas dan kebanggaan bagi bangsa Indonesia, dimana batik juga telah dikenal secara luas baik dalam skala nasional dan internasional. Peluang dalam kegiatan bisnis penjualan batik yang meningkat beberapa waktu ini dilihat oleh banyak pihak sebagai prospek yang menjanjikan. Penjualan batik secara umum di beberapa wilayah di Indonesia masih dilakukan dengan cara yang sederhana dan klasik. Untuk dapat menjangkau lebih banyak pelanggan serta memperluas area pemasaran sekaligus promosi batik, maka diperlukan media yang dapat menunjang kegiatan promosi, transaksi penjualan dan informasi batik dengan optimal. Salah satu media yang menjadi perhatian masyarakat sekarang ini adalah dengan menggunakan media website online. E-Commerce merupakan salah satu kegiatan transaksi bisnis baik barang dan jasa yang dilakukan secara elektronik dengan menggunakan jaringan internet CV Selaras Batik menyadari bahwa peranan media website dapat digunakan untuk meningkatkan omset penjualan dan juga sebagai media sarana dalam memasarkan produk batik yang mereka miliki untuk menjangkau pasar yang lebih luas lagi serta dapat meminimalkan biaya-biaya operasional seperti biaya iklan dan juga biaya sewa toko secara fisik. Penulisan paper ini bertujuan untuk menganalisa pengaruh dari penggunaan media website online dalam hal ini adalah e-commerce dengan menggunakan pendekatan B2C (Business-to- Customer dalam upaya meningkatkan transaksi penjualan batik pada CV Selaras Batik dan juga media penyampaian informasi baik pada para pelanggan dan masyarakat umum dalam memperoleh informasi mengenai produk-produk batik yang dipasarkan oleh CV Selaras Batik. 

  2. Mechanistic pathways differences between P25-TiO2 and Pt-TiO2 mediated CV photodegradation

    International Nuclear Information System (INIS)

    The Crystal Violet (CV) dye represented one of the major triphenylmethane dyes used in textile-processing and some other industrial processes. Various metals doped titanium dioxide (TiO2) photocatalysts have been studied intensively for the photodegradation of dye in wastewater treatment. In order to understand the mechanistic detail of the metal dosage on the activities enhancement of the TiO2 based photocatalyst, this study investigated the CV photodegradation reactions under UV light irradiation using a Pt modified TiO2 photocatalyst. The results showed that Pt-TiO2 with 5.8% (W/W) Pt dosage yielded optimum photocatalytic activity. Also the effect of pH value on the CV degradation was well assessed for their product distributions. The degradation products and intermediates were separated and characterized by HPLC-ESI-MS and GC-MS techniques. The results indicated that both the N-de-methylation reaction and the oxidative cleavage reaction of conjugated chromophore structure occurred, but with significantly different intermediates distribution implying that Pt doped TiO2 facilitate different degradation pathways compared to the P25-TiO2 system.

  3. PEMBUATAN SISTEM INFORMASI AKUNTANSI TERKOMPUTERISASI ATAS SIKLUS PEMBELIAN DAN PENJUALAN PADA CV. X

    Directory of Open Access Journals (Sweden)

    Andreas Handojo

    2004-01-01

    Full Text Available CV. X is still using a manual accounting system. They use a note for recording account receivable and account payable. Calculating total sales and profit do in manually. It takes a large amount of time to get some information that needed. Therefore an adequate accounting information system is needed in order to proceed an automate journalizing and to produce information accurately and quickly. First step in building accounting information system is to analyze and watch closely to the company's existing system. And the second step is to design the accounting information system, which can fulfill and appropriate with the company needs. The accounting information system is formed from integration of sales, purchasing and accounting/recording process. Including in sales are establishing sales order, checking available inventory for sale (stock and customer's credit limit. Including in purchasing is establishing purchase order until receiving and recording purchase invoice. Including in accounting/recording are journalizing, posting and reporting income statement. This software is using Microsoft SQL Server 7.0 as the database and Borland Delphi 7.0 as the compiler. Implementation for the whole software has gained 72.5% for user's satisfaction. Therefore it can be conclude that software has already fulfilled and appropriate with the needs of the company's system. But there also have some weakness to handle. This software helps in accelerating and simplifying the recording process and reporting income statement process. Abstract in Bahasa Indonesia : Sistem akuntansi pada CV. X masih menggunakan cara manual yaitu pencatatan piutang pada kartu piutang dan pencatatan hutang pada kartu hutang. Perhitungan total penjualan dan keuntungan dilakukan manual, sehingga membutuhkan waktu yang lama untuk mendapatkan informasi yang diinginkan. Oleh karena itu dibutuhkan sistem informasi akuntansi yang memadai, untuk memproses pencatatan secara otomatis dan

  4. Armazenamento de caqui (Diospyros kaki, L. cv. Quioto, em atmosfera controlada

    Directory of Open Access Journals (Sweden)

    DONAZZOLO JOEL

    2002-01-01

    Full Text Available Objetivou-se avaliar, com o presente trabalho, o efeito da temperatura e do CO2 no armazenamento em atmosfera controlada sobre a conservação de caqui cv. Quioto. Foram avaliadas as temperaturas -1,0 e -0,5ºC, e pressões parciais de CO2 de 0; 5 e 10kPa, com os tratamentos arranjados em um esquema bifatorial. Os frutos foram avaliados após 3 meses de armazenamento mais 3 dias de exposição à temperatura ambiente (18-20ºC. Conforme os resultados, não foi constatada interação entre os fatores, havendo efeito significativo para temperatura somente na firmeza de polpa, em que -1,0ºC apresentou frutos mais firmes. As diferentes pressões parciais de CO2 não influenciaram a perda de peso e a firmeza de polpa. As podridões apresentaram uma resposta linear negativa em relação ao CO2, porém, mantendo elevada ocorrência. Valores de CO2 entre 5 e 10kPa proporcionaram frutos com menor índice de escurecimento de epiderme e com coloração mais amarela e vermelha. O CO2 entre 5 e 10kPa apresentou os melhores resultados, que somados à temperatura de -1,0ºC, foi a melhor condição de armazenamento, que, no entanto, teve o período de conservação inferior a três meses nesta condição, devido às altas perdas por podridões.

  5. Structural and functional characterization of proteinase inhibitors from seeds of Cajanus cajan (cv. ICP 7118).

    Science.gov (United States)

    Swathi, Marri; Lokya, Vadthya; Swaroop, Vanka; Mallikarjuna, Nalini; Kannan, Monica; Dutta-Gupta, Aparna; Padmasree, Kollipara

    2014-10-01

    Proteinase inhibitors (C11PI) from mature dry seeds of Cajanus cajan (cv. ICP 7118) were purified by chromatography which resulted in 87-fold purification and 7.9% yield. SDS-PAGE, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF/TOF) mass spectrum and two-dimensional (2-D) gel electrophoresis together resolved that C11PI possessed molecular mass of 8385.682 Da and existed as isoinhibitors. However, several of these isoinhibitors exhibited self association tendency to form small oligomers. All the isoinhibitors resolved in Native-PAGE and 2-D gel electrophoresis showed inhibitory activity against bovine pancreatic trypsin and chymotrypsin as well as Achaea janata midgut trypsin-like proteases (AjPs), a devastating pest of castor plant. Partial sequences of isoinhibitor (pI 6.0) obtained from MALDI-TOF/TOF analysis and N-terminal sequencing showed 100% homology to Bowman-Birk Inhibitors (BBIs) of leguminous plants. C11PI showed non-competitive inhibition against trypsin and chymotrypsin. A marginal loss (<15%) in C11PI activity against trypsin at 80 (°)C and basic pH (12.0) was associated with concurrent changes in its far-UV CD spectra. Further, in vitro assays demonstrated that C11PI possessed significant inhibitory potential (IC50 of 78 ng) against AjPs. On the other hand, in vivo leaf coating assays demonstrated that C11PI caused significant mortality rate with concomitant reduction in body weight of both larvae and pupae, prolonged the duration of transition from larva to pupa along with formation of abnormal larval-pupal and pupal-adult intermediates. Being smaller peptides, it is possible to express C11PI in castor to protect them against its devastating pest A. janata. PMID:25093261

  6. Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus

    Directory of Open Access Journals (Sweden)

    Zadeh Shamila Yadolahi

    2015-09-01

    Full Text Available This study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpurea cv. Magnus, in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3 (0, 250, 500, 1000, and 1500 mg×L−1 with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1 GA3 with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1 GA3 for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3 showed higher viability and better performance under salinity stress condition.

  7. Effect of Fym, Urea and Azotobacter on Growth, Yield and Quality of Strawberry Cv. Chandler

    Directory of Open Access Journals (Sweden)

    Iqbal UMAR

    2009-06-01

    Full Text Available The present investigation was carried out in the Research orchard of Division of Fruit Science, Faculty of Agriculture, SKUAST-J , Udheywalla, Jammu during 2005-06 and 2006-07 to study the effect of organics FYM integrated with urea and Azotobacter on growth, yield and quality of strawberry cv. Chandler. The strawberry plants attained the height of 21.24cm with 28.16cm plant spread , 74.95cm2 leaf area and fruit size (37.62 x 28.01mm and fruit weight (15.87gwith the application of 25 per cent nitrogen through FYM augmented with Azotobacter and was at par with the plants supplied with cent per cent nitrogen in the form of urea in combination with Azotobacter. The fruit quality viz. total soluble solids, total sugars, ascorbic acid and anthocyanin content was highest in fruits obtained from plants supplied with 25 per cent nitrogen through FYM + 75 per cent nitrogen in the form of urea + Azotobacter recording 6.81 oBrix, 4.73 per cent, 73.71mg/100g fresh berries and 0.191 OD respectively. Maximum yield of 372.89g per plant was obtained with the application of cent per cent nitrogen in the form of urea along with Azotobacter whereas 358.43g fruits per plant were recorded with the application of 25 per cent nitrogen in the form of FYM + 75 per cent through urea + Azotobacter and were at par with each other.

  8. Scale-Dependent Measurements of Meteorite Strength and Fragmentation: Tamdakht (H5) and Allende (CV3).

    Science.gov (United States)

    Cotto-Figueroa, D.; Asphaug, E. I.; Garvie, L. A. J.; Morris, M. A.; Rai, A.; Chattopadhyay, A.; Johnston, J.; Borkowski, L.

    2015-12-01

    Meteorites are pieces of natural space debris, which have survived ejection from their parent bodies and passage through the Earth's atmosphere. As such, they provide a unique opportunity to study the fundamental physical and mechanical properties of early Solar System materials. But to date, few direct studies of physical properties have been conducted on meteoritic materials, in contrast to extensive chemical and isotopic analyses. It is important to determine these properties as they are related to disruption and fragmentation of bolides and asteroids, and activities related to sample return and hazardous asteroid mitigation. Here we present results from an ongoing suite of scale-dependent studies of meteorite strength and fragmentation. The meteorites studied are Tamdakht (H5), an ordinary chondrite that exhibits a heterogeneous structure criss-crossed with shock veins and centimeter-sized regions of white and light grey, and the carbonaceous chondrite Allende (CV3), which suitable pieces are light grey with abundant chondrules and CAIs. Uniaxial compression tests are performed on meteorite cubes ranging from 0.5 to 4 centimeters using an Instron 5985 frame with a 250 kN load cell and compression fixtures with 145mm diameter radial platens. All tests are conducted at room temperature and in displacement control with a displacement rate of 0.25 mm per minute to ensure quasi-static conditions. A three-dimensional digital image correlation (DIC) system that enables noncontact measurement of displacement and strain fields is also used. Analysis of the strength and failure process of the two meteorite types is conducted and compared to terrestrial materials.

  9. Rendimiento y componentes del valor nutritivo del Panicum maximum cv. Tanzania

    Directory of Open Access Journals (Sweden)

    Verdecia, Danis M.

    2008-05-01

    Full Text Available El trabajo fue realizado con el objetivo de determinar el rendimiento y algunos componentes del valor nutritivo del valor nutritivo del Panicum maximum cv. Tanzania a diferentes edades de rebrote, para esto se midió una parcela de 1600 m2 , aplicando un corte de uniformidad a 10 cm de altura del suelo. Las edades evaluadas fueron los 30, 45, 60, 75, 90 y 105 días. Se evaluaron el rendimiento, la composición química, la digestibilidad y energía metabolizable. Se empleó un diseño en bloques al azar con seistratamientos y cuatro repeticiones, aplicando un análisis de clasificación doble y las medias se compararon utilizando la prueba de rangos múltiples de Newman Keuls. El programa estadístico empleado fue el Statiics versión 6.0 para Windows. En los resultados se puede apreciar que el rendimiento en materia seca aumenta con la edad de la planta, con sus resultados más elevados a los 105 días con (12.7 tms/ha/corte, mientras que la proporción hoja – tallo, la proteína bruta, la digestibilidad de la materia seca y orgánicay la energía metabolizable disminuyeron con la edad con su mejorcomportamiento a los 30 días con (11.62%,63.5, 68.74% y 10.17 Mjrespectivamente, mientras que la fibra aumentó con la edad siendo susvalores más altos a los 105 días con (35.53% y .

  10. Development of bunchy top virus resistant banana cv lakatan in vitro culture and radiation technology

    International Nuclear Information System (INIS)

    Bunchy to virus (BTV) is the most destructive virus disease of banana in the Philippines. Incorporation of resistance to this virus disease by conventional hybridization is not possible due to male and female sterility of most commercial banana cultivars. In vitro culture coupled with radiation technology can be used to develop BTV resistance in banana cv. Lakatan. The sensitivity of banana shot tip explants to gamma irradiation was determined by subjecting the shoot tips to varying doses (5, 10, 20, 25, 30, 40, 60, 80 and 100 Gy) of irradiation. The LD sub 50 for banana shoot tips determined by 50% reduction in growth and shoot proliferation, was observed to around 20-25 Gy. Bulk irradiation of shoot tip explants was conducted using 20-25 Gy. Irradiated cultures were multiplied for 3-5 cycles and plants regenerated were potted out and screened for BTV resistance. A total of 3,447 irradiated plants regenerated from the radiosensitivity experiment (1,847 plants) and bulk irradiation of 20/25 Gy (1,600 plants) were screened for BTV resistance in the greenhouse using artificial BTV inoculation using the aphid vector Pentalonia nigronervosa. One hundred eighteen plants or 3.4% (118/3,447) of the artificially irradiated plants showed seedling resistance after 4-7 months of evaluation. These plants were planted in the field and were subjected to natural BTV infection. To date, 85 (out of the 118) putative seedling resistant plants continuously expressed BTV resistance in the field after more than 10 months of evaluation. The absence of BTV infection in 39 putative resistant plants was confirmed by ELISA test. Suckers from selected putative resistance plants will be collected, propagated and evaluated for the second cycle stability of BTV resistance and detailed characterization of important horticultural traits

  11. QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli

    Institute of Scientific and Technical Information of China (English)

    Asad Muhammad Azeem; BAI Bin; LAN Cai-xia; YAN Jun; XIA Xian-chun; ZHANG Yong; HE Zhong-hu

    2013-01-01

    The Italian wheat cv. Strampelli displays high resistance to powdery mildew caused by Blumeria graminis f. sp. tritici. The objective of this study was to map quantitative trait loci (QTLs) for resistance to powdery mildew in a population of 249 F2:3 lines from Strampelli/Huixianhong. Adult plant powdery mildew tests were conducted over 2 yr in Beijing and 1 yr in Anyang and simple sequence repeat (SSR) markers were used for genotyping. QTLs Qpm.caas-3BS, Qpm.caas-5BL.1, and Qpm.caas-7DS were consistent across environments whereas, Qpm.caas-2BS.1 found in two environments, explained 0.4-1.6, 5.5-6.9, 27.1-34.5, and 1.0-3.5%of the phenotypic variation respectively. Qpm.caas-7DS corresponded to the genomic location of Pm38/Lr34/Yr18. Qpm.caas-4BL was identified in Anyang 2010 and Beijing 2011, accounting for 1.9-3.5%of phenotypic variation. Qpm.caas-2BS.1 and Qpm.caas-5BL.1 contributed by Strampelli and Qpm.caas-3BS by Huixianhong, seem to be new QTL for powdery mildew resistance. Qpm.caas-4BL, Qpm.caas-5BL.3, and Qpm.caas-7DS contributed by Strampelli appeared to be in the same genomic regions as those mapped previously for stripe rust resistance in the same population, indicating that these loci conferred resistance to both stripe rust and powdery mildew. Strampelli could be a valuable genetic resource for improving durable resistance to both powdery mildew and stripe rust in wheat.

  12. Effects and action mechanisms of Korean pear (Pyrus pyrifolia cv. Shingo) on alcohol detoxification.

    Science.gov (United States)

    Lee, Ho-Sun; Isse, Toyoshi; Kawamoto, Toshihiro; Woo, Hyun-Su; Kim, An Keun; Park, Jong Y; Yang, Mihi

    2012-11-01

    Korean pear (Pyrus pyrifolia cv. Shingo) has been used as a traditional medicine for alleviating alcohol hangover. However, scientific evidence for its effectiveness or mechanism is not clearly established. To investigate its mechanism of alcohol detoxification, both in vitro and in vivo studies were performed with an aldehyde dehydrogenase 2 (ALDH2) alternated animal model. The pear extract (10 mL/kg bw) was administered to Aldh2 normal (C57BL/6) and deficient (Aldh2 -/-) male mice. After 30 min, ethanol (1 g or 2 g/kg bw) was administered to the mice via gavage. Levels of alcohol and acetaldehyde in blood were quantified by GC/MS. First, it was observed that the pears stimulated both alcohol dehydrogenase (ADH) and ALDH activities by 2∼3-  and 1.3-fold in in vitro studies, respectively. Second, mouse PK data (AUC(∞) and C(max) ) showed that the pear extract decreased the alcohol level in blood regardless of ALDH2 genotype. Third, the pear increased the acetaldehyde level in blood in Aldh2 deficient mice but not in Aldh2 normal mice. Therefore, the consistent in vitro and in vivo data suggest that Korean pears stimulate the two key alcohol-metabolizing enzymes. These stimulations could be the main mechanism of the Korean pear for alcohol detoxification. Finally, the results suggest that polymorphisms of human ALDH2 could bring out individual variations in the effects of Korean pear on alcohol detoxification. PMID:22451246

  13. Effects of Rooting Substrates on In Vitro Rooting of Anthurium andraeanum L. cv. Avanti

    Directory of Open Access Journals (Sweden)

    Wararat KEATMETHA

    2004-06-01

    Full Text Available A study was made of the effects of rooting substrates on in vitro rooting of Anthurium andraeanum L. cv. Avanti, orange flower. Initiation of root was attempted in several rooting substrates with modified ½ MS medium supplemented with 30 g/l sucrose. The cut end of the shoot was dipped in 2.5 g/l indole-3-butyric acid (IBA before insertion in substrates. After 4 weeks of culture, it was found that roots were markedly induced in 8 and 12 g/l agar with 86.67 and 73.33% in root induction rate, 14.62 and 12.41 mm in root length, with 3.54 and 3.91 roots in root number respectively. However, 93.33% of root induction rate with 3.00 roots, and 11.66 mm in root length were produced on medium containing 1.5 g/l phytagel while peat moss and vermiculite could induce rooting at 46.67% with 7.86 and 6.66 mm in length, with 1.00 and 1.86 roots respectively. Root could not be induced in sphagnum moss. Anatomical study of root showed no abnormality in all rooting substrates. The roots formed in high concentrations of agar at 12 and 16 g/l, 2.0 and 2.5 g/l phytagel, and peat moss were thicker than those formed in other rooting substrates. Especially, root formed in peat moss had the largest vascular diameter. However, roots formed in phytagel, peat moss, and vermiculite had more root hairs than those formed in agar substrate. Plantlets, rooted in peat moss and vermiculite, survived at 100% after acclimation in a mixture of soil and coconut husk.

  14. Nutritional characteristics and K and Mg fertilizer combination in Baxi banana%巴西蕉的营养特性及钾镁肥配施技术研究

    Institute of Scientific and Technical Information of China (English)

    姚丽贤; 周修冲; 彭志平; 陈婉珍

    2005-01-01

    钾、镁肥配施是香蕉施肥的重要措施之一.研究巴西蕉(Musa AAA Giant Cavendish cv.Baxi)的营养特性及钾镁肥配施对其生长影响的田间试验结果表明,假茎与叶片是养分累积的最主要部位;在整个生育期,叶片Mg/K比波动最大.为获得60t/hm2的高产,巴西蕉需要吸收N 275.3 kg、P 24.6kg、K 900.0kg、Ca 151.2kg、Mg 73.2kg、S 23.9kg、Fe 2091.7g、Mn2910.6 g、B 228.6 g和Zn 435.6 g.在土壤镁素丰富及Mg/K比为6.87和香蕉大量施钾条件下,施镁肥对巴西蕉的叶片营养、植株长势、蕉果农艺性状、品质及产量没有明显影响,因此,在此条件下可不必配施镁肥.

  15. Self Consistent Simulation of C-V Characterization and Ballistic Performance of Double Gate SOI Flexible-FET Incorporating QM Effects

    CERN Document Server

    Azim, Zubair Al; Niaz, Iftikhar Ahmad; Alam, Md Hasibul; Ahmed, Imtiaz; Khosru, Quazi D M

    2012-01-01

    Capacitance-Voltage (C-V) & Ballistic Current- Voltage (I-V) characteristics of Double Gate (DG) Silicon-on- Insulator (SOI) Flexible FETs having sub 35nm dimensions are obtained by self-consistent method using coupled Schrodinger- Poisson solver taking into account the quantum mechanical effects. Although, ATLAS simulations to determine current and other short channel effects in this device have been demonstrated in recent literature, C-V & Ballistic I-V characterizations by using self-consistent method are yet to be reported. C-V characteristic of this device is investigated here with the variation of bottom gate voltage. The depletion to accumulation transition point (i.e. Threshold voltage) of the C-V curve should shift in the positive direction when the bottom gate is negatively biased and our simulation results validate this phenomenon. Ballistic performance of this device has also been studied with the variation of top gate voltage.

  16. Presence of elevated non-HDL among patients with T2DM with CV events despite of optimal LDL-C - A report from South India.

    Science.gov (United States)

    Kumpatla, Satyavani; Soni, Anju; Narasingan, S N; Viswanathan, Vijay

    2016-01-01

    Elevated non-high density lipoprotein cholesterol (non-HDL-C) was the commonest lipid abnormality among T2DM patients with cardiovascular events (CV) events. Prevalence of elevated non-HDL-C was 21.6% among patients who were on statin therapy and with optimal low density lipoprotein-cholesterol (LDL-C) levels. Despite an optimal LDL-C level, 47% of the T2DM patients with CV events had elevated non-HDL-C.

  17. Construction of a subgenomic CV-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells.

    Science.gov (United States)

    Wehbe, Michel; Huguenin, Antoine; Leveque, Nicolas; Semler, Bert L; Hamze, Monzer; Andreoletti, Laurent; Bouin, Alexis

    2016-04-01

    Coxsackieviruses B (CV-B) (Picornaviridae) are a common infectious cause of acute myocarditis in children and young adults, a disease, which is a precursor to 10-20% of chronic myocarditis and dilated cardiomyopathy (DCM) cases. The mechanisms involved in the disease progression from acute to chronic myocarditis phase and toward the DCM clinical stage are not fully understood but are influenced by both viral and host factors. Subgenomic replicons of CV-B can be used to assess viral replication mechanisms in human cardiac cells and evaluate the effects of potential antiviral drugs on viral replication activities. Our objectives were to generate a reporter replicon from a cardiotropic prototype CV-B3/28 strain and to characterize its replication properties into human cardiac primary cells. To obtain this replicon, a cDNA plasmid containing the full CV-B3/28 genome flanked by a hammerhead ribozyme sequence and an MluI restriction site was generated and used as a platform for the insertion of sequences encoding emerald green fluorescent protein (EmGFP) in place of those encoding VP3. In vitro transcribed RNA from this plasmid was transfected into HeLa cells and human primary cardiac cells and was able to produce EmGFP and VP1-containing polypeptides. Moreover, non-structural protein biological activity was assessed by the specific cleavage of eIF4G1 by viral 2A(pro). Viral RNA replication was indirectly demonstrated by inhibition assays, fluoxetine was added to cell culture and prevented the EmGFP synthesis. Our results indicated that the EmGFP CV-B3 replicon was able to replicate and translate as well as the CV-B3/28 prototype strain. Our EmGFP CV-B3 replicon will be a valuable tool to readily investigate CV-B3 replication activities in human target cell models. PMID:26800776

  18. The role of seed size in the non-genetic variation exhibited in salt tolerance studies involving the bread wheat cv. chinese spring

    Directory of Open Access Journals (Sweden)

    P. K. Martin

    2014-02-01

    Full Text Available The intention of this study was to confirm the role of seed size in the non-genetic variation exhibited during salinity tolerance experiments involving the bread wheat cv. Chinese Spring. The nutrient film/rockwool hydroponics technique was utilised. This study concluded that seed size does not play a significant role in the non-genetic variation generated during a study of salinity tolerance of the bread wheat cv. Chinese Spring.

  19. Drought tolerant M4 segregants of soybean Cv. JS 335 and CO (Soy)3

    International Nuclear Information System (INIS)

    The cultivation soybean (Glycine max L.), crop is constrained by the inadequate moisture availability during the spring and / or summer season, especially at the pod initiation and pod filling stage. Evolution of drought tolerant cultivars through hybridization is tedious because of the very fragile and small sized chasmogamous and cleistogamous flowers of soybean. More over only a very narrow genetic base is available in the germplasm of soybean maintained in India. Hence an attempt was made to irradiate the soyabean seeds of the Cv. JS 335 and CO (Soy 3) with gamma rays and Ethyl Methane Sulphonate (EMS) to induce heritable variability for drought tolerance. The seeds of the above mentioned cultivars were irradiated with gamma rays 250 Gy dose, EMS with a concentration of 0.2 and 0.4 per cent, combinations of 100 Gy + 0.2 per cent EMS and 100 Gy + 0.4 per cent of EMS. The source used for gamma irradiation was Cobalt 60, available at Indira Gandhi Centre for Atomic Research, Kalpakkam, India. Single plant M3 and M4 families segregants were observed with different root densities, leaf thickness with higher grain yield, under imposed drought stress conditions compared to the parental check cultivars. Macro mutants viz., dwarf, mutants with lanceolate leaflets, little leaf, tall and twiny mutants with broader leaves were observed and isolated. A lanceolate leaf mutant from 0.2 per cent EMS treatment having a mean leaf thickness 31.9 dm was observed to be drought tolerant in M4 generation compared to the mean leaf thickness of 25.8 dm in the control (Co.SOY 3). Similarly a long petiole leaf mutant with thicker leaves of 37.4 dm compared to the leaf thickness of 29.8 dm in the control (JS335) was also observed in the 250 Gy gamma irradiation. In this study it is observed that gamma irradiation is more efficient in inducing leaf thickness compared to that of chemical mutagen. (author)

  20. Produtividade do Panicum maximum cv. Mombaça irrigado, sob pastejo rotacionado

    Directory of Open Access Journals (Sweden)

    Müller Marcela dos Santos

    2002-01-01

    Full Text Available O uso da irrigação em pastagens é uma realidade fundamentada na experiência empírica de produtores, devido à falta de bases científicas definidas sobre o assunto. O trabalho visou avaliar a produtividade do capim Mombaça (Panicum maximum Jacq. cv. Mombaça manejado em pastejo rotacionado, sob sistema de irrigação e as principais variáveis climáticas responsáveis pelo acúmulo de massa de forragem. O experimento foi conduzido sob irrigação por aspersão tipo pivô central, de julho a dezembro de 1998, em região de cerrado (Latossolo Vermelho-Amarelo em São Desidério, BA. Foi quantificada a massa de forragem da pastagem aos 30 dias de crescimento e antes do pastejo e desenvolvidos modelos matemáticos que relacionam a produção de forragem a variáveis climáticas. A produção e a taxa de acúmulo de matéria seca da pastagem, ao longo do período de inverno não apresentaram diferenças significativas. Na primavera, houve tendência de aumento em ambas, com maiores produções obtidas no período final de avaliação. As menores taxas de acúmulo durante a primavera foram observadas nos primeiros piquetes, coincidindo com a ocorrência de baixas temperaturas durante o período de descanso. Com a elevação da temperatura mínima, a produção forrageira entrou em fase de acúmulo crescente. A produção de forragem, durante a primavera, foi superior à do inverno, apresentando incrementos em função da maior temperatura mínima do ar, do período de descanso e da área foliar inicial. As principais variáveis climáticas responsáveis pela produção da forragem foram temperatura mínima do ar e disponibilidade de água no solo.